LOCUS       DATLEE010000001         6197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2235880, whole genome shotgun sequence.
ACCESSION   DATLEE010000001 DATLEE010000000
VERSION     DATLEE010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235880"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VM692_00005"
     CDS             <1..449
                     /locus_tag="VM692_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUQ50566.1"
                     /translation="TDVTSSLLALGAGISWAVSVIVFKRMPVDSRNDLLSVTAWQMVF
                     GMIPLVVIALLVEERPVVWSGYFIGALIYNGVAGMAIATLLWLYILQRLPATISGLNA
                     LAVPIVGVIAAWIQLGERPSPAEGLGMLLVLAGLALLTRAQRNVQP"
     gene            complement(515..904)
                     /locus_tag="VM692_00010"
     CDS             complement(515..904)
                     /locus_tag="VM692_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HUQ50567.1"
                     /translation="MGRKLYVGNLPFSATEEDITAKFSACGTVESAKLITDRDTGRSK
                     GFGFVEMSSDSEAQAAIDKLNGQDYGGRPMTVNEARPQEKKGPRPGGGGGYGGGGGGG
                     GRGGGGGGRGGYGGGGGGYGGGGGYNR"
     gene            1095..1700
                     /locus_tag="VM692_00015"
     CDS             1095..1700
                     /locus_tag="VM692_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50568.1"
                     /translation="MRRKPLFRSLTHLFIAAITLAAAAQTEPRELLNSERIAAEFGSY
                     GVAVLEQDDAVRVSNLFSGAGDERTCRTFAIVRYSERIGPEIAEEHAAIVAGGSLGAT
                     FAARGWEVRKTHLRYGERPASQRLADLMRVAVGTRLAWHAYVLDVVKGDRTIEYAALV
                     EVHHPDYLVLADLSAIYGPPAVDSRRELVANLLATADERLR"
     gene            complement(1697..2485)
                     /locus_tag="VM692_00020"
     CDS             complement(1697..2485)
                     /locus_tag="VM692_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HUQ50569.1"
                     /translation="MAEHSTSASEAWRGYRPAAGDFADRVVMVTGATAGIGRAVAREL
                     VRAGASVILHGRNEKALEALYQELKPLGPEPSVAQLDLERAQGPEYQALTAAIEGRYG
                     RLDGLLHNAAILGDRSPIEHYDIGLWQRVLLVDLTAPFILTRCLLPLLRNSADASVLF
                     TTSGVGHRGRAYWGAYAAAKAGTENLAEVLADELESTSIRVNVINPGRTRTRMRARAY
                     PAENPASVPTPESAAPTYLYLLGAASRGVHGQRVEVQEPSVPAR"
     gene            complement(2485..3774)
                     /locus_tag="VM692_00025"
     CDS             complement(2485..3774)
                     /locus_tag="VM692_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HUQ50570.1"
                     /translation="MTADVGLYVALAGIGGIAIGLLVGLWRAMGLTRELASKDAELRA
                     RAAADVEREQVVTLAAERLGSAFDQLADRQFHSHSETFLKLARETLSAQTERAKGDLA
                     AREQAIDGLVSPIREALARAERQLLELDQSRRETHGSISAQLEALAASQQALSAETRN
                     LVGALRRPEVRGQWGEITLRRLVELAGMVEHCDFVSQRHQPTETGAVRPDMIIHLPEA
                     RDLVVDVKTPLDAYLEATEAPGDAERRTALERHATIVAGRIRELAGKAYWSQFERSPE
                     FVILFIPGDQFLSAALAERPTLLDDALRQNIILATPTSLVALLKAVAYGWQQTSLAQN
                     AAEIRTSALQLYERLTTFASHLGNVGKSLGDSVRAFNASVGSLERMVLPSARRFTDLG
                     VQPRQRMVPPKSIEELTRDVAAAVDSPSAAETADGPH"
     gene            complement(3771..4466)
                     /gene="ubiG"
                     /locus_tag="VM692_00030"
     CDS             complement(3771..4466)
                     /gene="ubiG"
                     /locus_tag="VM692_00030"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HUQ50571.1"
                     /translation="MSNVDSSELAKFASRAEEWWDRRGAFRTLHEINDLRLEYIETRA
                     PVAGHRVLDVGCGGGLLAEGLATRGAHVVAIDMAPENIAAARQHAATGVLTIDYRCVD
                     VEQLAREMAGQFDVVTCLEMLEHVPQPSRIVAACAAALRPGGHAFFSTINRNLKSFAL
                     AIVGAEYVLGLLPRGTHEYAKLIRPAELATWCRGVGLDVAELTGLHHNPATAAYWLDG
                     NVDVNYFAWAVRE"
     gene            complement(4474..5787)
                     /locus_tag="VM692_00035"
     CDS             complement(4474..5787)
                     /locus_tag="VM692_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HUQ50572.1"
                     /translation="MQTIDALICPRWTVRVEPTVVAEEGLALAIDGGRIIDVLARGEA
                     ERSYAPRVRHDRTSHVLLPGLVNAHTHAAMTLFRGLADDLPLDSWLRERIWPAEARWI
                     GPDFIADGTRLAVAEMLRGGITCFADMYYHPDVTGAVAADAGMRVVLGMIALDFATSW
                     AKDAEEYLRKGLEVHDRFKAEPLVTTAFAPHAPYSVGDATLQRIRRLADELDVPVHMH
                     VHETAAEVGAAVAETGRRPLARLAALGLVTPALIGVHATELTADEIEQLATSGASVVH
                     CPRSNLKLASGLCPVAKLLAAGVNVALGTDGAASNNRLDLWSEMQTAALLAKHGAGSA
                     AALPAATALRMATINGARALGLAGEIGSLAAGKAADVICVDLSGPEHRPLLDPLSQLV
                     YAASRHDVTDVWVAGEHLVAERTPLRLDVAEICTAAERWGRQLRA"
     gene            6046..>6197
                     /locus_tag="VM692_00040"
     CDS             6046..>6197
                     /locus_tag="VM692_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HUQ50573.1"
                     /translation="MAVGKEVLLVNLEDEMRQSYLDYAMSVIVGRALPDVRDGLKPVH
                     RRVLHA"
BASE COUNT         1004 a         2060 c         2047 g         1086 t
ORIGIN      
        1 cgacggacgt gacgagcagc ttgctggcgc tcggcgccgg catttcgtgg gccgtcagcg
       61 tcatcgtgtt caagagaatg cccgtcgata gccgcaacga cctattgtcc gttacggcgt
      121 ggcagatggt attcggcatg attccgctcg tcgtgattgc tttgctcgtc gaggagcggc
      181 cggtcgtttg gagcggctac ttcatcggcg cattgatcta taacggcgtc gccggaatgg
      241 cgatcgcaac gctgctgtgg ctctacatcc tgcagcgctt gcctgcgacg atcagcggtc
      301 tcaacgcgct cgcggtgccg atcgtcggcg tgatcgcagc ttggattcag ctaggagagc
      361 ggccgagccc cgccgaaggc ctcgggatgc tattggtgct cgcggggctc gcgctgctga
      421 cgcgcgcgca gcgcaacgtt cagccgtaga aaaaaacggg cgacctcttt cagggtcgcc
      481 cgtcgttcga tgcgtttctc gtccaagctc gacgttagcg attgtagccg ccgccaccgc
      541 cgtagccgcc accgccaccg ccgtagccac cgcggccgcc gccaccgccg ccgcggccac
      601 cgccgccacc gccgcctcca tagccgccgc caccacccgg gcgagggccc ttcttttcct
      661 gaggacgtgc ttcgttgaca gtcatcgggc gaccgccgta gtcctgacca ttgagcttgt
      721 cgatggccgc ttgagcttcc gagtcggagc tcatctcgac gaacccgaag cctttgctgc
      781 gcccggtgtc acgatccgta atgagcttgg ccgattcgac ggtaccgcac gccgagaact
      841 tagcggtgat gtcttcttca gtcgcagaga acggcaagtt gccgacgtag agtttcctgc
      901 ccacgagacc tccaaaaaaa ttttaaggca aaccgtttaa ggaggaatct gggtgctagg
      961 ccctcaggct cgatgaacgg ttacgattcg atcttagtat atacctcaaa catttctcat
     1021 tgacacggcc aagtttttaa ggtttctcca gtaacatcgc gcgcaacgcg ggaccgtttt
     1081 ggcacgcaac acgcatgcgt cgtaaaccgc ttttccgatc gctgacacat ctatttattg
     1141 cggcaattac gctcgccgct gccgcgcaaa cggagccccg tgagctactg aacagcgagc
     1201 gcatcgcggc tgagttcggc agctacggtg tggcggtcct ggagcaggac gatgcggttc
     1261 gcgtctcgaa ccttttcagc ggggcgggcg acgaacgaac gtgccgcacg ttcgcaatcg
     1321 tgcgctattc cgagcgcatc ggtccggaaa tcgccgagga gcatgccgcg atcgttgcgg
     1381 gcggttcact gggtgccacc ttcgctgcgc gaggttggga agtccgcaag acgcatctgc
     1441 gctacggcga gcggcccgcc tcgcagaggc tagcggacct gatgcgggtt gcagtgggca
     1501 ctcgcctggc gtggcacgct tacgtgctcg acgtcgtcaa aggcgaccgc acgatcgaat
     1561 atgccgcgct cgtcgaagtt catcaccccg attacctcgt gctcgcggac ctctcggcga
     1621 tctacggtcc gcccgccgtc gatagtcgtc gcgagctcgt ggcgaacttg ctggcgacgg
     1681 cggacgagcg cttacgctag cgcgccggga cgctcggctc ctgcacttcg acacgctgcc
     1741 cgtgaacgcc gcggctcgcc gccccgagca agtagaggta cgtgggcgcg gcgctttcgg
     1801 gcgtcgggac cgaggcggga ttctcggcag ggtaggcccg cgcgcgcata cgagtgcgcg
     1861 tgcgtccggg attgatcacg ttgacgcgaa tcgatgtgct ttcgagctcg tcggccagca
     1921 cttcggcgag attttccgtg ccggctttgg ccgccgcata ggccccccag tacgcgcggc
     1981 cgcggtggcc gacgccgctc gtcgtgaaca gcacggatgc gtcggcggag ttgcgcaaca
     2041 acggtaacag gcagcgcgtc aggatgaacg gcgccgtcag gtcgacgagc aacacgcgct
     2101 gccagaggcc aatgtcatag tgctcgatcg ggctgcgatc gccgaggatc gctgcgttgt
     2161 gcagcaaccc gtcgagccgg ccgtagcgtc cttcgatcgc ggccgtcagc gcttggtact
     2221 cgggaccttg tgcgcgctcg agatcgagct gcgcgaccga cggctccggg ccgagcggct
     2281 tgagctcctg atacaacgcc tcgagcgctt tctcgttgcg gccgtgcagg atgacgcttg
     2341 cgccggcccg cacgagctcg cgggccacgg cgcggccgat gccggccgtg gctcccgtga
     2401 ccatgaccac gcggtctgcg aaatcgcccg cggccggtcg atagccgcgc cacgcttcgc
     2461 tcgcgcttgt cgaatgctcg gccatcaatg cggcccgtcg gcggtttcgg ctgcgcttgg
     2521 cgagtcgacg gcggccgcga cgtcacgcgt cagctcctcg atcgacttcg gcggcaccat
     2581 gcgctgtcgc ggctgcacac cgagatcggt gaagcgccgc gcgctcggca acaccattcg
     2641 ttcgagcgag ccgacgctcg cgttgaaagc gcgcacgctg tcgccgagcg acttgccgac
     2701 attgccgagg tggctcgcga aagtcgtcag acgctcgtag agctgtaacg ccgaggtgcg
     2761 gatctccgcg gcattctgcg cgagcgacgt ctgctgccag ccgtacgcca cggctttcag
     2821 cagcgcgacg aggctcgtcg gcgtagccag gatgatgttc tgccgcagcg catcgtcgag
     2881 caacgtcggg cgctcggcca gcgctgcgct caagaattgg tcgcccggaa tgaacaggat
     2941 cacgaactcc gggctccgct cgaactggct ccagtaagcc ttgcctgcga gctcgcggat
     3001 ccgccccgcg acgatcgtcg catgccgttc gagcgccgtg cgccgctccg cgtcgccggg
     3061 cgcctcggtc gcctcgaggt aggcgtcgag cggcgtcttg acgtcgacga cgagatcgcg
     3121 ggcttccggg aggtgaatga tcatgtctgg acgtacggcg cccgtttccg tcggctgatg
     3181 tctttggctc acgaagtcgc aatgctcgac catgccagca agctcgacga gccggcgtag
     3241 cgtgatctcg ccccactggc ctcggacctc gggccgacgc aacgcgccga cgaggttgcg
     3301 cgtctcggcg ctcagcgctt gctggctggc cgcaagggct tcgagctgcg cgctgatgct
     3361 gccgtgcgtc tcgcgccgcg attgatcgag ctcgagcaac tgccgctccg cgcgcgcgag
     3421 ggcttcgcgt atcggactca cgagaccgtc gatcgcttgc tcgcgtgccg cgagatcgcc
     3481 tttggcgcgc tcggtttgcg cgctcaaggt ctcgcgcgcg agcttcagga atgtctccga
     3541 gtgactgtgg aattgccgat ccgcaagctg atcgaacgcc gacccgagcc gctccgccgc
     3601 gagcgtgacg acttgctcgc gctcgacgtc ggccgcagca cgagcgcgaa gctccgcgtc
     3661 cttcgacgcg agctcgcgcg tcaaacccat cgcacgccag aggccgacca gcaggccgat
     3721 cgcgattccg ccgatgccgg ccaacgcaac gtacagcccg acgtcggccg tcactcacgc
     3781 accgcccacg cgaagtagtt cacgtcgacg ttgccgtcga gccaatacgc ggcggtcgca
     3841 gggttgtggt gcagccccgt gagctccgcg acgtcgaggc cgacgccgcg gcaccacgtt
     3901 gcgagctcgg ccgggcgaat cagcttggcg tactcgtgcg tgccgcgcgg cagtaacccg
     3961 agcacgtact cggcgccgac gatggccagc gcgaagctct tcaagttgcg attgatcgtc
     4021 gagaagaacg cgtggccgcc ggggcgcagc gccgcggcgc aggcagcgac gatgcggctc
     4081 ggctgcggca cgtgctcgag catctcgagg cacgtcacga cgtcgaactg cccggccatt
     4141 tcgcgcgcca gctgctcgac gtcgacgcac cgatagtcga tcgtcagcac cccagttgcc
     4201 gcatgctggc gtgcggccgc gatgttttcg ggcgccatgt cgatcgcaac gacgtgcgcg
     4261 ccgcgcgttg ccaaaccctc ggcgagcaag ccgccgccac agccgacgtc gagcacccgg
     4321 tggccggcga ccggcgcgcg agtctcgatg tactcgaggc gtaggtcgtt gatctcgtgc
     4381 agcgtacgga acgcgccgcg tcggtcccac cactcctcgg cgcgggacgc gaacttggcg
     4441 agctcactgg agtcgacgtt cgacatcgtt acctcaagcg cgcagttgcc ggccccaacg
     4501 ttccgctgcc gtgcagatct ccgcgacgtc gagacgcagc ggcgtgcgct ccgcgacgag
     4561 atgctcgccg gccacccaaa cgtcggtgac gtcgtgcctc gaggcagcgt aaaccagctg
     4621 cgacaacgga tcgagcaacg gccggtgctc ggggccagac aggtccacgc aaatgacgtc
     4681 ggccgctttg ccggccgcaa gcgagccgat ctcgccggcg agcccgagcg cacgagcgcc
     4741 gttgatcgtc gccatgcgta atgccgtcgc ggcgggcagg gcggccgcgc tgccggcgcc
     4801 gtgctttgcc agcagggccg ccgtctgcat ctcggaccag aggtcgagcc ggttgttgct
     4861 cgctgcgcca tcggtgccga gcgccacgtt gacgccggcg gcaagcaatt tcgcgacggg
     4921 gcaaagaccg cttgcaagct tcaagttcga gcgcgggcaa tggacgacgc tcgctccgct
     4981 ggtcgcgagc tgctcgatct cgtcggcggt gagctccgtc gcatgtacgc cgatcagtgc
     5041 cggcgtcacg agccctaacg ccgcgaggcg agccaaaggg cgccggccgg tttccgctac
     5101 cgcagcgccg acctcggccg cggtctcgtg cacgtgcatg tgcacgggaa cgtcgagctc
     5161 gtcggcgaga cggcgtatac gctgcagcgt ggcatcgccg acgctgtacg gtgcatgcgg
     5221 tgcaaacgcc gtcgtgacca gcggctcagc cttgaaacgg tcgtgcactt ccaagccttt
     5281 gcgcaagtac tcttcggcgt ccttggccca ggaggtcgcg aagtcgagcg cgatcatgcc
     5341 gagcacgacg cgcatgccgg cgtcggccgc gacggcgccc gtgacgtccg ggtggtaata
     5401 catgtcggcg aagcaggtga tgccgccgcg cagcatctcg gcaaccgcga gccgcgtgcc
     5461 gtcggcgatg aagtccggcc cgatccatcg cgcctcggcc ggccagatcc gctcgcgcag
     5521 ccagctgtcg agcggcaagt cgtccgcaag cccgcgaaac aacgtcatcg cggcgtgagt
     5581 atgcgcgttc acgagccccg gcagcagcac gtgactcgtg cggtcgtggc gtacgcgcgg
     5641 cgcgtagctc cgctcggctt cgccgcgagc caagacgtcg atgatgcggc ccccgtcgat
     5701 cgcgagggca agaccttcct cggctacgac cgtcggctcg acgcgtacgg tccatcgcgg
     5761 acagatcagg gcgtcgatcg tttgcatcgg caggtagagc gctcgaaaaa aaaggcgagc
     5821 aagtatagct ttccggggcc acttcggcgc tacggacgcc agcttccgtg tgctagaatt
     5881 cggccttgtt ttttgggcac ttggaagcgt tctcggggtg atgcaagagc cgcataaacg
     5941 gtcgtcggcg ttgcccgaaa acttaatacc tcgtcaactt tggatcccct ccgcgccgtc
     6001 gcgggcgggt ggagggcggg gccgtatttt tgttgagctc tagtaatggc cgtaggtaag
     6061 gaagtcctgt tagtcaatct cgaagacgag atgcgccagt cgtatctcga ttacgcgatg
     6121 agcgtaatcg tgggccgcgc gctaccggac gtccgcgacg gcctgaagcc cgtgcatcgg
     6181 cgggtattgc acgcgat
//
LOCUS       DATLEE010000002         5365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7454275, whole genome shotgun sequence.
ACCESSION   DATLEE010000002 DATLEE010000000
VERSION     DATLEE010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7454275"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VM692_00045"
     CDS             complement(<1..392)
                     /locus_tag="VM692_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HUQ50574.1"
                     /translation="MNKAKTSTVFEAFFVPRVRGLREAAFWVLAGLSLILLLALLSYS
                     PTDPAFSVAGGDDAVTNRMGPAGAWFADISFLLFGASAYLFPILVLIAGVFLFRGDEL
                     PEKGPVVWRGTGFLLAIATSSGLATLHF"
     gene            515..1675
                     /locus_tag="VM692_00050"
     CDS             515..1675
                     /locus_tag="VM692_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ50575.1"
                     /translation="MRLQLERLSSIGSVEAAAWNALAGDNPFLKHEFLAALEQSGCVG
                     DGTTWQPCYLVARDDRGLAGALPLFIKYDSHGEFVFDWSWADAYERSGRSYYPKLVAA
                     IPFTPATGQRLLLRDGLESGVANELLDEARNTAREIGASSLHVLFPTEPERQTFEGAG
                     FLARKACQFHWANGGYRDFDEFLATFSADKRKKAKRERRRVAEADIVFEHLRGDEPSR
                     ADWDAIVDFYSKTFWRRGREPYLNRGFFERIAATMPANLVIVLARRHGVPIATAICFR
                     SSTALYGRYWGSSADFHSLHFETCYYQGIDYCIREGLQRFEPGTQGEHKVARGFVPQP
                     TWSCHWLRDPEFHRAVGAFLTRETRHVDAYMDEIGEHVPYRQASRDDALIDA"
     gene            1675..2388
                     /gene="aat"
                     /locus_tag="VM692_00055"
     CDS             1675..2388
                     /gene="aat"
                     /locus_tag="VM692_00055"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HUQ50576.1"
                     /translation="MKRERTRSLLWLSRNQRVDAFPPASEALTEPNGLLAAGGDLEPE
                     RLLAAYRQGIFPWYSEGQPILWWSPDPRAVLWPNGLRVSRSLRRSLRRRGFEFRVDTE
                     FERVVAACAEPRRYASGTWITAEMAAAYARLHEQGWAHSFETWAEGRLVGGLYGVAIG
                     RAFFGESMFTRVTDASKVALAHAVEFLRARGTEIIDCQVASAHTQSLGAVDIPRAQFL
                     ALIAELCAEAAPPQTWRFA"
     gene            2462..2680
                     /gene="infA"
                     /locus_tag="VM692_00060"
     CDS             2462..2680
                     /gene="infA"
                     /locus_tag="VM692_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HUQ50577.1"
                     /translation="MAKEEAIQMEGTVVETLPNTTFRVELENGHVVTAHISGKMRKHY
                     IRILKGDAVTVELTPYDLTKGRIVYRAR"
     gene            complement(2693..5023)
                     /gene="clpA"
                     /locus_tag="VM692_00065"
     CDS             complement(2693..5023)
                     /gene="clpA"
                     /locus_tag="VM692_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HUQ50578.1"
                     /translation="MLSSELEYCLNDAFQRAREERHEFITVEHLLLALLDTPTVIETL
                     KACGTDLARLRRELKEFIEGSTPRLRGEDDEGTEVQPTLGFQRVLQRAVFHVQSSGRK
                     EVTPINVLVAIFGEKQSQAVYLLGLQDVTRLDVVNFISHGVPKVQEASKAEPDEAAAD
                     GDRQAESNPLERFTTNLNAQAEAGKIDPLIGRALEIERTVQILCRRRKNNPLFVGEAG
                     VGKTALAEGLARLIVEGKVPDILRPCVIYALDMGSLIAGTKYRGDFEKRLKGVVAGLK
                     KRPGAILFIDEIHTVIGAGAASGGVMDASNLIKPLLANGELRCIGSTTYSEFRGIFEK
                     DHALARRFQKIDVLEPSVAETVEILRGLKTRFEEHHSIQYSDEALRAAAELSAKHIND
                     RHLPDKAIDVIDEAGANCRLQPEGSRPRIVDVDLIQTIVAKIARIPPKNVSASDRDVL
                     RNIERDLKLVIFGQDRAIGALASAIKMARSGLGDERRPVGSFLFSGPTGVGKTEVTRQ
                     LALTLGVELVRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAITKHPHCVLLFDE
                     IEKAHPEVFNLLLQVMDHGTLTDNNGRKADFRNVTIVMTTNAGAQESSRASVGFTLQD
                     HSTDAMEILKKLFSPEFRNRLDAIIQFEPLDEQSVARVVDKLVVELEAQLDKNEVTIE
                     LDPAARDWVAARGYDKKMGARPMARVIQEHIKRPLAEELLFGKLTEGGHVRVEVAADG
                     QSLLLTVEPLKRELEDLRGKGSPPRELAGRPGERDD"
     gene            complement(5023..5352)
                     /gene="clpS"
                     /locus_tag="VM692_00070"
     CDS             complement(5023..5352)
                     /gene="clpS"
                     /locus_tag="VM692_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009022289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HUQ50579.1"
                     /translation="MSQDSEKGDRADDHGLATAEAKPRLKRPPLYRVILLNDDYTPME
                     FVVEVLQKIFSLDRTTATRIMLEVHTKGKGVCGVYTYEIAETKVAQVTGLAQQHQHPL
                     LCTMEET"
BASE COUNT          930 a         1750 c         1705 g          980 t
ORIGIN      
        1 tgaaagtgca gtgtcgcgag accggagctc gtcgcgatcg ccagcaggaa accggtgcca
       61 cgccagacca cgggcccttt ctcgggcaac tcgtcgccgc ggaacaggaa gacgccggcg
      121 atcaagacca gtatcgggaa cagataagcc gaagcgccga acagcaggaa cgagatgtcg
      181 gcgaaccaag cgcccgcggg tcccatacgg ttcgtcacgg cgtcatcgcc gcccgcgacg
      241 ctgaacgccg ggtccgtcgg gctatagctc aacagggcca acagcaaaat caggctcaag
      301 ccggccaata cccagaacgc cgcctcgcga agccctctga cgcgcgggac gaagaaggcc
      361 tcgaaaacgg tgctggtttt ggctttattc aagttgaaca gcagttacgc gacttaatgc
      421 gctgattata ctgataacct cggctacgcc tagtccgccg cggcgagcgg cgtcccggct
      481 cgacgctcgc agcagcgacg ggcacactcg acgcatgcgg ctgcaactcg aacgactgtc
      541 ttcgatcggc tccgtcgagg cggcggcttg gaacgcgctc gccggcgaca acccgttcct
      601 gaagcacgag ttcttggccg cgctcgagca gagcggctgc gtcggtgacg gcacgacctg
      661 gcaaccttgc tacctcgtcg cccgcgacga ccgggggctc gccggcgcgt tgccactctt
      721 tattaagtac gactcgcacg gcgagttcgt cttcgattgg agctgggccg acgcctacga
      781 gcggtcgggc cgcagctact acccgaagct cgtcgcggcg atcccgttca ctccggcgac
      841 gggccagcgc ctgttgctac gcgacgggct cgagagcggc gtggcgaacg agctgctcga
      901 cgaagcgcgc aacaccgcac gcgagatcgg cgcctcgtcg ttgcacgtct tgttcccgac
      961 agagccggaa cggcaaacgt tcgagggcgc ggggttcctc gcgcgcaagg cctgccagtt
     1021 tcactgggcg aacggcggct accgcgattt cgacgagttc ctcgctacgt tcagcgccga
     1081 caagcgcaag aaggcgaaac gcgagcgccg ccgagtcgcc gaggccgaca tcgtcttcga
     1141 gcatctgcgc ggcgacgagc cttcgcgcgc cgattgggac gcaatcgtgg acttctactc
     1201 gaaaacgttc tggcgccgcg gccgagagcc gtatttgaat cgcggcttct tcgagcgcat
     1261 cgcggcgacg atgcccgcca atctcgtcat cgtgctcgcg cgccggcacg gcgtgccgat
     1321 tgcgactgcg atctgcttcc gcagcagcac ggcactgtac ggccgctatt ggggcagcag
     1381 cgccgatttt cacagcctgc acttcgagac gtgctactac caaggcatcg actactgcat
     1441 tcgcgagggg ttgcagcgct tcgagcccgg cacgcagggc gagcacaaag ttgcgcgcgg
     1501 attcgtgccg caaccgacgt ggtcgtgcca ttggctgcgc gatcccgagt ttcatcgcgc
     1561 tgtcggcgca ttcctgacgc gcgagacgcg ccacgtcgac gcgtatatgg acgagatcgg
     1621 cgagcacgtc ccgtatcgac aagcctcgcg cgacgacgca ttgatcgacg cttgatcaag
     1681 cgcgagcgca cgagatccct gctctggctc tcgcgcaacc agcgcgtcga cgcgtttcca
     1741 ccggcgagcg aggccctgac cgagccgaac gggctgctgg cggccggcgg ggacctcgag
     1801 cccgagcgcc tgctcgccgc ctatagacaa ggaatctttc cgtggtattc ggagggccaa
     1861 ccgatcctct ggtggagtcc cgatccgcgg gccgtgctct ggccgaacgg cctgagagtc
     1921 tcgcgcagcc tgcgccgctc gctgcggagg cgggggttcg agtttcgtgt cgacaccgag
     1981 ttcgagcgcg tcgtcgccgc ctgcgccgag ccgcggcgtt atgccagcgg tacctggatc
     2041 accgcggaga tggcggccgc ctacgcccgc ctgcacgagc aaggctgggc gcactcgttc
     2101 gagacttggg cggaaggccg gctcgtcggc ggactttatg gcgtcgcaat aggccgtgcg
     2161 ttcttcggcg aatcgatgtt cacgcgggtc acggatgcct cgaaggtcgc gctcgcgcac
     2221 gcggtcgagt tcttgcgcgc acgcggcacc gaaatcatcg actgtcaggt cgcctcggca
     2281 cacacgcaga gcctcggcgc cgtcgacatt ccgagagcgc agttcctggc gctgatcgcg
     2341 gagctttgcg ccgaagccgc accgccgcag acgtggcgtt tcgcgtgaac tgccgtttgc
     2401 tgctgttgct ccccttatgc aacaatgcgc cgccctgacg ggggctccca actgttgcgg
     2461 aatggcaaaa gaagaagcaa ttcaaatgga aggtacggtg gtggaaacgc tgccgaacac
     2521 tacctttcgc gtcgagctcg agaacggtca cgtggtgacc gcgcacatct cgggcaagat
     2581 gcgtaaacac tacattcgca ttttgaaagg cgatgccgtc accgtggagc tcacgcccta
     2641 cgacctgacg aaagggcgca tcgtctaccg cgcccgctag ctcggcgcgt cgtcagtcgt
     2701 cgcgctcgcc gggtcgcccg gccagctcgc gcggcggcga tcctttgccg cgcagatcct
     2761 cgagctcccg cttcagcggc tcgaccgtca gcaacaagct ttggccgtcg gcggcaacct
     2821 cgacgcgcac gtgcccgccc tcagtcagct tgccgaacag cagctcttcc gcgagcggcc
     2881 gcttgatgtg ctcctgaatc acgcgagcca tgggccgcgc gcccatcttc ttgtcgtagc
     2941 cgcgagcggc cacccaatcg cgcgcagcgg gatcgagctc gatcgtgact tcgttcttgt
     3001 cgagctgcgc ctcgagctcg acgacgagct tgtcgacgac gcgcgcgacc gattgctcgt
     3061 cgagcggctc gaactgaatg atcgcgtcga gacgattgcg gaactccggc gagaacagct
     3121 tcttcaagat ctccattgcg tccgtcgagt gatcctgcag cgtgaacccg accgacgcgc
     3181 ggctcgactc ctgtgcaccg gcgttcgtcg tcatgacgat cgtgacgttg cggaagtcgg
     3241 ccttgcggcc gttgttgtcg gtcagcgttc catgatccat gacctgcagc agcaggttga
     3301 aaacctcggg atgcgccttc tcgatctcgt cgaataggag cacgcagtgc ggatgcttcg
     3361 tgatggcctc ggtcagcagc ccgccttggt cgaagccgac atatccgggc ggtgcaccga
     3421 tcaaacgcga cacggtgtgg cgctccatgt actcggacat atcgaagcgc acgagctcga
     3481 cgccgagagt caacgccagc tgccgagtga cctcggtctt gccgacgccg gtcgggcccg
     3541 agaacaagaa cgaccccacg ggccggcgct cgtcgccgag ccctgagcgc gccatcttga
     3601 ttgcggacgc gagcgcgccg atcgcgcgat cttgcccgaa gatcacgagc ttcaagtcgc
     3661 gttcgatatt gcgtagcacg tcgcggtcgg aggccgacac gttcttcggc ggaatgcgcg
     3721 cgatcttggc gacgatggtt tggatgaggt cgacgtcgac gattcggggc cggctgccct
     3781 ccggttgcag gcggcagttc gcgccggctt cgtcgatgac gtcgatggcc ttgtcgggca
     3841 gatgacggtc gttgatgtgc ttcgcggaca gctcggcggc cgcgcgcaac gcctcgtccg
     3901 agtactggat gctgtggtgc tcctcgaagc gggtcttcaa gccgcgcaag atctcgacgg
     3961 tctcggcgac ggagggctcc agcacgtcga tcttctgaaa gcgcctcgcg agcgcgtgat
     4021 ctttctcgaa gatgccgcgg aactcgctgt aggtcgtcga gccgatgcag cgcagctcgc
     4081 cgttcgcgag cagcggcttg atgaggttcg aagcgtccat gacgccgccg gacgccgcgc
     4141 cggcgccgat cacggtgtgg atctcgtcga tgaacaagat cgcgccgggg cgctttttga
     4201 gcccggccac gacgcccttc aatcgcttct cgaagtcgcc gcggtacttg gtgccggcga
     4261 ttaacgagcc catgtcgagc gcgtagatca cgcatggccg cagaatgtcg ggcaccttac
     4321 cttcgacgat caggcgcgcg agcccttccg caagcgcggt cttgccgacg ccggcttcgc
     4381 cgacgaacag cgggttgttc ttgcggcgac ggcacaggat ctgcaccgtg cgctcgatct
     4441 cgagcgcacg gccgatcaac gggtcgatct tgccggcctc ggcctgcgca ttgagattgg
     4501 tcgtgaagcg ctcgagcgga ttcgactccg cttgccggtc gccgtcggcc gccgcttcat
     4561 cgggctcggc tttgctcgcc tcctgcacct tcggcacgcc gtgcgagatg aagttgacga
     4621 cgtcgagccg cgtcacgtcc tgcaagccca acaggtagac ggcctgcgac tgcttctcgc
     4681 cgaagatggc gacgagcacg ttgataggcg tgacttcctt acggccgctc gactggacgt
     4741 ggaacaccgc gcgttgcagc acgcgctgga aacccagcgt gggctgcact tcggtgccct
     4801 cgtcgtcctc gccgcgcaga cgcggcgtcg aaccttcgat gaactccttg agctcgcggc
     4861 gcagacgggc gaggtccgtg ccgcacgcct tcagcgtctc gatgacggtc ggcgtatcga
     4921 gcagcgcgag caacaagtgc tcgacggtga tgaattcatg gcgttcctcg cgcgcgcgct
     4981 ggaacgcatc gttcaagcaa tattcgagct cacttgacag cattacgttt cttccatggt
     5041 gcaaagcagc ggatgctggt gctgttgcgc aagacccgtg acctgcgcaa ctttcgtctc
     5101 cgctatctca tatgtataga caccgcacac accctttccc ttggtatgca cctcgagcat
     5161 gatgcgggtc gccgtcgtgc gatctaacga gaaaattttc tgtaatacct cgaccacaaa
     5221 ctccatcggc gtgtagtcgt cgttcaacaa aatcacgcgg taaagcggtg gcctctttag
     5281 acgcggttta gcttctgccg ttgccagccc gtgatcgtcg gcgcggtcgc ctttttccga
     5341 atcctgtgac atgtgtcggg aattc
//
LOCUS       DATLEE010000003         3905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5219470, whole genome shotgun sequence.
ACCESSION   DATLEE010000003 DATLEE010000000
VERSION     DATLEE010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5219470"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /gene="gspK"
                     /locus_tag="VM692_00075"
     CDS             complement(<1..559)
                     /gene="gspK"
                     /locus_tag="VM692_00075"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HUQ50580.1"
                     /translation="MSLRRQQSGVALVMAVLVVAIGTMIAVNLMWEGTLDLRRAEAAL
                     ASDQGLLYVQGAEAWAADILREDLVDSPDSDHLGELWAVELAPLPVDGGTITGRLEDL
                     QGRFNLNNLIGADGKENQLARRQFERLLNSVEIDPQLAGAVIDWLDPDTDLRFPTGGE
                     DVVYTSQDPPYRTANSMITSTSELMA"
     gene            complement(556..1176)
                     /gene="gspJ"
                     /locus_tag="VM692_00080"
     CDS             complement(556..1176)
                     /gene="gspJ"
                     /locus_tag="VM692_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HUQ50581.1"
                     /translation="MTRRPQTSRGFTLIELLVAIAILAIIGVMAFVGLNRVIDQQQLA
                     RARTERWQDVQLAMRLVVQDLAQLHPRATREELGESYQPSMLADPSAQFALEFSRGGW
                     ANPAGLPRGTVLRVAYDWEDDKLVRWHWTVMDRTLSTPPIRTEILDRVTNVEVRFLDT
                     AGEWHLEWPPVDRRGPQSLITRPRAVEFAIELEDFGRVYRLVETAG"
     gene            complement(1173..1622)
                     /gene="gspI"
                     /locus_tag="VM692_00085"
     CDS             complement(1173..1622)
                     /gene="gspI"
                     /locus_tag="VM692_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HUQ50582.1"
                     /translation="MRRQASTGFTLIEVMVALVIVSLGMMAVNTQLNRYTVAATYVEE
                     KTLASWIATNKLTELSIAPTWPAVGDYEEDVEFAGQQWRCQIVVEETPVTNLHRVDVS
                     VRLLTDPERVVHKVSALIEPPAPPGFLSPQWSAPPGNGGRRGSGERG"
     gene            complement(1626..2192)
                     /gene="gspH"
                     /locus_tag="VM692_00090"
     CDS             complement(1626..2192)
                     /gene="gspH"
                     /locus_tag="VM692_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HUQ50583.1"
                     /translation="MPTSVTGTYRSPAALCKEAGFSLLELLVVVAIIGLLAGAITLSL
                     GALGNDRELQEETNRLRSVIDLLHEESLMQSRDYGVMFTSTGYRFYVYDYQQLMWVLP
                     QTDRLLQQHALRPQLALSLILDGRDVPLERDFEEQDVENAEPQIMLLASGELTPFTIE
                     MQRDGMEGRFELTAALDGEVTVAEKGFN"
     gene            complement(2164..2601)
                     /gene="gspG"
                     /locus_tag="VM692_00095"
     CDS             complement(2164..2601)
                     /gene="gspG"
                     /locus_tag="VM692_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HUQ50584.1"
                     /translation="MRVIRTPLRVAEQGFTLLEIMVVVVIIGLLAAIVAPSFVGQIDK
                     AAVNRAKTDIRGIETALNLYRLDNFRYPSTTEGLQALVKNPGEANAPNWRQQLQRTPV
                     DPWEQPYQYAYPGQHGDFDVFSYGADRQEGGEGINADIGNWDL"
     gene            2743..3297
                     /gene="rrtA"
                     /locus_tag="VM692_00100"
     CDS             2743..3297
                     /gene="rrtA"
                     /locus_tag="VM692_00100"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HUQ50585.1"
                     /translation="MLTIFVVCLALAAGGDALREVGRYQRDAIEGGQYWRLVSGHLVH
                     LGLGHLWPNLAALLVIGALFDDVFRSVDWLRASLASAAAIDVGLYVLDPDVIWYVGLS
                     GVLHGLVAAGAFALLLRRQKIGAVLAAGLGAKLAFEQLAGPLPFTAESVGGPVVVAAH
                     LYGAAGGLLAEAVAHVVRRRRSQV"
     gene            3330..>3905
                     /locus_tag="VM692_00105"
     CDS             3330..>3905
                     /locus_tag="VM692_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ50586.1"
                     /translation="MDSKSLPIFPGIAGRTALVTGATRGIGRAIAEMLAQQGAVVVGT
                     ATSEQGAASISSWLGAFSPAGRGVVLDVSSDASVEALFASLSSQPEIVVNNAGITRDN
                     LLMRMKAEEWNDVVSTNLSSVYRVCKACMRGMMKARHGRIINISSVVGLMGNAGQTNY
                     AAAKAGMIGFTKSLAREVASRNVTVNAVAPGF"
BASE COUNT          659 a         1259 c         1262 g          725 t
ORIGIN      
        1 cggccatcaa ctccgaggtc gacgtgatca tggagttcgc cgtgcggtac ggtggatcct
       61 ggctcgtgta gacgacgtcc tcgccgccgg tcgggaagcg caggtcagtg tccgggtcca
      121 accagtcgat gacggcgccc gcgagctgcg gatcgatctc gacgctgttc aaaaggcgct
      181 cgaactgccg gcgcgcgagc tggttctcct tgccgtcggc gccaatcaga ttgttcaagt
      241 tgaagcgccc ctgcaggtcc tcgagcctcc ccgtgatcgt gccgccgtcg acgggcagcg
      301 gcgccagctc gacggcccac agctcgccga gatgatcgct gtcgggcgaa tcgaccaagt
      361 cttcccgcag aatgtcggcc gcccaggctt ccgcgccctg gacgtagagc aagccctgat
      421 cggacgcgag cgccgcttcc gcgcgccgca aatcgagcgt tccttcccac atcaaattca
      481 cggcgatcat cgtgccaatc gcgacgacga gcacggccat gaccaacgcg actccgcttt
      541 gctgccggcg caagctcatc ccgccgtctc caccaagcga tacacgcggc cgaagtcttc
      601 gagctcgatc gcgaattcga cggcgcgcgg ccgcgtgatc aaactctgcg ggccgcggcg
      661 gtcgacgggc ggccactcga ggtgccactc tccggccgta tcgagaaatc ggacctcgac
      721 gttcgtcacg cggtcgagga tctcggtgcg tatgggcggc gtcgacagcg tgcgatccat
      781 gacggtccag tgccaacgca cgagcttgtc gtcctcccag tcgtacgcga cgcgcagcac
      841 ggttccgcga ggaaggccgg ccggattggc ccagccgccg cggctgaact cgagcgcgaa
      901 ctgcgcgctc gggtccgcga gcatgctcgg ctgatagctc tcgccgagct cctcgcgcgt
      961 cgcacgcgga tgcagctgcg cgagatcctg caccacgagc cgcatcgcga gctgcacgtc
     1021 ttgccagcgt tccgtgcgcg ccctcgcgag ctgttgctgg tcgatgacgc ggttcaagcc
     1081 gacgaatgcc atgacgccga tgatcgcgag gatcgcgatc gcgacgagca gctcgatcaa
     1141 cgtgaagccg cggctcgttt gaggccggcg cgtcatccgc gctctccact gccgcgccga
     1201 ccgccgttgc ccggcggcgc cgaccattgc ggcgacaaaa agcccggcgg ggcagggggc
     1261 tcgatcagcg ccgagacttt gtgcacgacg cgctcgggat ccgtgagcag ccgcaccgac
     1321 acgtcgacgc ggtgcaggtt cgtcacgggc gtctcttcga cgacgatctg acaacgccat
     1381 tgttggccgg cgaactccac gtcttcctcg tagtcaccga cggccggcca cgtcggcgcg
     1441 atgctgagct ccgtcagctt gttcgtcgcg atccaactcg cgagcgtctt ctcctcgaca
     1501 taagtcgccg caaccgtgta gcgattcagc tgcgtgttca cggccatcat gccgagcgag
     1561 acgatgacga gggcgaccat gacctcgatg agcgtgaagc ctgtcgaagc ttggcgccgc
     1621 atgcgtcagt tgaaaccttt ctcggccacg gtgacctcgc cgtcgagcgc tgccgtcaac
     1681 tcgaaacgcc cctccattcc gtcacgctgc atctcgatcg tgaacggagt cagctcgcca
     1741 cttgcgagca acatgatctg cggttccgcg ttctcgacgt cttgctcttc gaagtcgcgc
     1801 tcgagcggca cgtcgcggcc gtcgagtatc agcgacaacg caagctgcgg gcgcaacgcg
     1861 tgctgctgca gcaagcgatc ggtctgcggc agcacccaca tcagctgttg atagtcgtac
     1921 acgtagaagc gatagcccgt cgacgtgaac atgacgccgt aatcgcggct ctgcatcagc
     1981 gactcctcgt gcagcaagtc gatgacgctg cgcagccgat tggtctcctc ctgaagctcg
     2041 cggtcgttgc cgagcgcgcc caacgacaag gtaatggcgc cggcgagcaa gccgatgatt
     2101 gcgacgacga cgaggagctc gagcagcgag aaaccggcct ccttgcagag cgccgcagga
     2161 ctcctatagg tcccagttac cgatgtcggc attgatacct tcaccgcctt cctggcgatc
     2221 cgcgccgtag gagaaaacgt cgaagtcgcc gtgctgcccc ggatacgcat attggtaggg
     2281 ctgctcccac ggatcgacgg gcgtgcgttg cagctgctgc cgccaattcg gcgcgttcgc
     2341 ctcgccggga ttcttcacga gcgcttgcag cccttccgtg gtgctcgggt aacgaaaatt
     2401 gtcgagtcga tagagattca gcgccgtctc gatgccgcga atatcggtct tcgcccggtt
     2461 gaccgccgcc ttgtcgattt gaccgacgaa gctcggggcg acgattgcgg cgagcaagcc
     2521 gatgatcacg acgacgacca tgatttcgag caacgtaaaa ccttgctcgg cgacgcgaag
     2581 aggtgttctg atcacgcgca ttgagtctac ctcggctgaa gccggcgggt tgccgaagaa
     2641 tcggaccgaa gatagcacaa gaggttcacg cgggttgtga aggtagagcc catttcgcgg
     2701 ccatccgcgg tcgcttttcg gcgactccga aactggtcga tagtgctaac gatcttcgtc
     2761 gtttgtttgg cattggctgc cggcggcgac gcgctgcggg aggtcggccg ttatcagcgc
     2821 gatgcaatcg aaggcgggca gtactggcgc ctcgtctcgg ggcatttggt gcacttgggt
     2881 ctaggccacc tctggccgaa ccttgccgcg ctgttggtca tcggggcgct gttcgacgac
     2941 gtgttccgca gcgtcgattg gttacgcgca agtcttgcgt cggcagcagc gatcgacgtc
     3001 ggcttgtatg tcctcgatcc cgacgtgatt tggtacgtcg ggctgtcggg cgttttgcac
     3061 ggcctcgtcg cggctggggc gttcgcgctg ttgctgcgtc gacaaaagat cggcgcggtg
     3121 ctcgcagccg gcctcggcgc gaagctcgcg ttcgagcagc tcgcggggcc cctgccgttc
     3181 acggccgaat cggtcggcgg gcctgtcgtc gtcgccgctc atttgtatgg tgcagccggc
     3241 ggtttgctcg cggaggccgt ggcgcacgtc gttagacgcc gccgttcgca ggtataatcc
     3301 cgcgccttgt cgattccggc gaggttgcaa tggattccaa gtcgttaccg atttttcccg
     3361 gcattgccgg acgcaccgcg ctcgtgacgg gagccacgcg cggcatcggc cgagcgattg
     3421 ccgagatgct ggcgcagcaa ggcgccgtcg tcgtcggcac ggcgacgagc gagcagggcg
     3481 ctgcttcgat ctcgagctgg ctcggcgcgt tctcgccggc gggtcgcggt gtcgtgctcg
     3541 acgtcagcag cgatgcgtcg gtcgaggcat tgttcgcgag cttgtcgagc caacctgaga
     3601 tcgtcgtcaa caacgccggc atcacgcgcg acaatctgct gatgcgcatg aaggcggaag
     3661 agtggaacga cgtcgtttcg acgaatctgt cgtcggtgta ccgagtttgc aaagcatgca
     3721 tgcgcggcat gatgaaagcg cgtcacggac gcatcatcaa catcagctcc gtcgtgggct
     3781 tgatgggcaa cgcgggccaa accaactacg cggcagcgaa ggcgggcatg atcggcttca
     3841 cgaagtcgtt ggcgcgcgag gtcgcgtccc gcaacgtcac cgtcaacgcc gtcgcgccgg
     3901 gcttc
//
LOCUS       DATLEE010000004         7831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3387, whole genome shotgun sequence.
ACCESSION   DATLEE010000004 DATLEE010000000
VERSION     DATLEE010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3387"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VM692_00110"
     CDS             complement(<1..1551)
                     /locus_tag="VM692_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HUQ50587.1"
                     /translation="MWPTRDIYAQRSCDQWHADVTAVEGRVEVRRVARQTWAVLATGE
                     RVCSGDTLRSAASSRATFTLIDGTTLSLDEHSVLGTPEPASGDGSLIELLRGVIHIIS
                     RDPRLLLFTTPYANAGLEGTEFDIRVDEAARSTEVVVLEGAVSVTTPAGAISVPSDHI
                     AVAKEGEAPTAMPYADPIEAMRWASYFPPLIDAALPGADQEPIAAQLADADFYAQRAA
                     ARLTTARIAAAEADIESALRIDSRNANALSLSALLSLARADRDTARRFLDDALAAEPS
                     SVVARLAASHLEQSSAQLAAAEHLLREAAALEPDNTIVLTRLAEIALGRGDSKAAIAS
                     AMRARDLAPAQSEPLVVLGFASLRAFDTAAAEHAFAAAIEREPNLPMAHLGAGLTATQ
                     RGDLAEGRRQLELAVALNPANPLARSYMAKFYDAENRGKLTTSQLELAKEFDPGDPTP
                     WLYSSLHRLRSNQPVEALQDLQAASRRNGDRPVFRSSLSIEEDVATASAGIGRVYNEL
                     GFSRLALNE"
     gene            1868..4036
                     /locus_tag="VM692_00115"
     CDS             1868..4036
                     /locus_tag="VM692_00115"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUQ50588.1"
                     /translation="MGLIGLVASLIPSTFELEEFLGLNVLFRLRGAVEPPAEVVIVGI
                     SREAARALGQTEELDTWPRELHAQLVDRLAAAGVTTIAFDLIFSEPREGPGDELFAKS
                     IAKAGNVLLLEETSESSVVPLGGVSTGRLEIRTPPLPALGAAALGTAPFILPEVPVRV
                     SQSWTFDRVTDETPSLPVLALQAHLLAYYEEFARLLERARPGSTSQWPATGAAVQEQR
                     SLERTVGALRRTLRGDVALRSAALEQLRGGNYPIATQNALLALLDLYSGTSSQLLNFY
                     GAARAIHTVPYDQALKGTDGIDWTGKVVLVGMSEPVQPRQQDDFISVFSQNTGINLSG
                     VEVGATRLANLLQRHTLRTLPLSQQVALVVVLGVVLGALVGRLTMLRASAVVAIGAAL
                     YFAAAYWQFSSHYVWLPLVVPLLLQLPLGFGAAVWWNYRDVSQQRERVQTALGYYVPP
                     SLARRLSEQTVSAGTNRELLHGTCLVTDAEHYTSIAERLSPAELAVLMNDYYESVFRV
                     VQAYGGEISDTAGDSMIAVWASAEPDAAVRLRAAEASLAILETVAAFNREHPQTPLPT
                     RIGLESGEMLLGNIGAEQRYEYRAIGDIVNTAARIQGLNQPLGTRVLISAATLDEQAR
                     LPSRDVGTFLLRGKRLPVRVVEPLSAATCALGDEELSKFAAALAAFRAGSWREAHERF
                     APLAERFFDDGPSRYYDATARAFLTEPPASWTGAIRLTSK"
     gene            complement(4055..4327)
                     /locus_tag="VM692_00120"
     CDS             complement(4055..4327)
                     /locus_tag="VM692_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50589.1"
                     /translation="MRTVERLPSEDQDKILRMVDLLTLVPLTVQNRTQRMLRELLEQQ
                     PNTKRECVARVDDVLAYLERNAASSGQQRDSVTRFDFAATERVRPS"
     gene            4572..4745
                     /locus_tag="VM692_00125"
     CDS             4572..4745
                     /locus_tag="VM692_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50590.1"
                     /translation="MTKVRNLLLIALVMMPAVTLNASSLNNAEPRPGVGAPLQVTGSC
                     WVYMNGRWYLLPC"
     gene            complement(4752..5492)
                     /locus_tag="VM692_00130"
     CDS             complement(4752..5492)
                     /locus_tag="VM692_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HUQ50591.1"
                     /translation="MRQINDSDDGSPAPAHLSPKAVLRHNYLFRGLSDATLEGIAALA
                     AKRTYYKSSVIFSQGGVGDSLFGVASGRVRISASASGGREVFLNIMEPGDTIGEIAVM
                     DGLPRTANAVSLDTSTLIVIQRTDFLPFLEREPQLAIHLLKLMCERLRWTSELVEESA
                     FMAGPARLAKRLLILASLHGRAARVGHLELRISQGELARFLGISRQIVNQHLADWRRN
                     GWIELGRTQIVIRNTEALRGLIAETNSV"
     gene            5617..>7831
                     /locus_tag="VM692_00135"
     CDS             5617..>7831
                     /locus_tag="VM692_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUQ50592.1"
                     /translation="MIKTPWRLAGRFLARDWRSGEVLVLLAALVVAVAAMSAVTFFTD
                     RVRAAVSQQAGEALAADLRVESINPLPAEIREAATRRGLATADVVSFRSVVAAGETTS
                     LADVRGVTAGYPLRGVVQVADRLAGVPEDASGVPPRGEVWAEPSLMARLGTAVGDDLE
                     IGQLRLKITRTLEFRPDEGWRLMEIAPTVLLSYDDVLASGLLAPGSIAQYVGLYAGEA
                     ADVEAFRRELPSLLRPQDRIEDFRNGRPEVGAAIANAERFLVLAALVSVLLGGVAVAM
                     AARRFVARRLDAVALMKCLGARHREVLQLNLLQLLILVVAAGVLGCLIGLAAQFGLTA
                     LLADFIEAQLPPPSLQGAVLGPLTALAVAIGCALPPLLQLGGVPPARVLRNDVGPPPL
                     RYTTIYGVAAVAVTALMYVLFGDVELIAYLLGGAIATFFVLYLAGRLLVLALQRLRGG
                     VGVAWRYGIANVARRGRESSVQVVAFGIGLMVLLLLTSVRTELMAEWQATLPANAPNH
                     FLINIQPAERAALAATLVAAGVPEPEMTPLVRARMSHINGVPVEQYQAATDFGRRELD
                     DETNLTWSADLPPDNELASGAWWGGNPDVPQVSLEEGLREGIGLKLGDEITYAIGGES
                     LTVRLTSTRTVHWESFRPNFFMVLSPGAIEGFAHTYITSLYLLPEQRRVTVDLVRQYP
                     SVSVLDIGAVIEQVRRSMDRAALAVQYVFLFTLAAGVMVLLAAIQATRDERRFESA"
BASE COUNT         1227 a         2468 c         2670 g         1466 t
ORIGIN      
        1 ctcattcagc gctagccggc tgaacccgag ctcgttgtat acgcggccga tgcctgcgct
       61 ggcagtcgca acgtcctctt cgatcgagag tgaggaacgg aaaacgggcc gatcgccgtt
      121 tcgccgcgag gcagcttgca aatcctgcaa cgcctcgacg ggctgattgc tgcgaagtct
      181 gtgcaacgag gaatataacc aaggagtcgg atcgccggga tcgaattcct tcgcgagctc
      241 gagttggctc gtggtcagct tgccgcggtt ctccgcgtcg tagaacttcg ccatgtagct
      301 gcgcgccaag ggattcgccg gattgagggc gactgcgagc tcgagctgac ggcgtccttc
      361 cgctaggtcc ccgcgttgcg tcgcggtgag cccggctccg agatgcgcca tcggcaaatt
      421 cggctcgcgc tcgatcgctg ccgcgaacgc atgctccgcg gctgccgtat cgaaggctcg
      481 caggctggca aaaccgagca cgacgagcgg ctcgctttgc gccggcgcca agtctcgcgc
      541 ccgcatcgcg ctcgcgatgg ccgctttcga gtcgcctctg ccgagcgcga tctccgcgag
      601 gcgcgtcagc acgatcgtgt tgtcgggctc gagcgctgcg gcttcacgga gcaggtgttc
      661 ggcggcggcg agttgtgccg agctctgctc gagatgcgac gccgcgagtc gcgccaccac
      721 ggacgacggc tcggcggcca gagcgtcgtc gaggaaccgc cgcgcggtgt cacggtcggc
      781 gcgcgccaga gacaggaggg cgctcagcga cagcgcgttc gcgttgcgcg agtcgatgcg
      841 gagcgcgctc tcgatgtcgg cttctgccgc tgcgatgcgc gcggtcgtga gtcgtgcggc
      901 cgctcgttgg gcgtagaaat ccgcgtcggc gagttgagcg gcgatcggct cttggtcggc
      961 gcccggaagc gccgcgtcga tgagcggcgg gaagtagctc gcccagcgca tagcctctat
     1021 gggatcggcg tagggcattg ctgtgggcgc ttcgccttcc ttggcgaccg cgatgtgatc
     1081 gctcgggacg ctgatcgcgc ctgcgggcgt cgtgaccgag acggcgcctt caagcacgac
     1141 gacttcggtt gaacgcgcgg cctcgtcgac gcgaatgtcg aactcggtgc cctcgaggcc
     1201 ggcgttggcg tatggcgtcg tgaacagcaa caggcgcgga tcgcggctga tgatgtggat
     1261 gaccccgcgc agcagctcga tcaacgagcc gtctccggac gccggctcgg gcgtgccgag
     1321 tacgctgtgc tcgtcgaggc tcaaggtcgt gccgtcgatc aaagtgaagg tcgcgcggct
     1381 cgacgccgcg ctgcgcagcg tatcgcccga gcagacgcgc tcgcccgtag cgagcacggc
     1441 ccaggtctgt cgcgccactc gtcgcacctc gacgcgacct tcgacggccg tgacgtcggc
     1501 gtgccactga tcgcagctgc gttgcgcgta gatgtcgcgc gtcggccaca acgccgcggc
     1561 gatcgcgagg gccagtgtcg cgcgggcgga aagcgggaga cggcgccggg aggcgcgtcg
     1621 agacgacggt cgcagcgagc ttgtttgtgg tggcgaacta gccatcaacg cccttattgt
     1681 tggcgcaacc acccgcggca gcggcttgca agccgcccgc ggaaccaacg agccgcgctt
     1741 tgtagtggag ctaacaatca gactagtccc gtgatgtgtc aagtccgcca cgcttcccgt
     1801 catgcccccg tccgcgccgg cctcgtctcc tagtcgggcg cggcgaagcc tttggctcgg
     1861 cctcacgttg gggctcatcg gcctcgtcgc gagcctgata ccgtcgacct tcgagctcga
     1921 agagttcctc gggctcaacg tcttgtttcg ccttcgcgga gccgtcgagc cgcccgccga
     1981 agtcgtcatc gttggaattt cgcgtgaggc ggctcgcgcg cttggccaaa cggaggagct
     2041 cgatacttgg cctcgcgagc tgcacgcgca gctagtcgat cgcttggcgg ctgccggcgt
     2101 gacgaccatc gcattcgacc tcattttctc ggagccgcga gaagggccgg gggatgagct
     2161 gttcgccaag tccatcgcaa aggccggcaa tgtcttgctc ttggaggaga cgtcggagag
     2221 cagcgtcgtg ccgctcggag gcgtttcgac ggggcgcctg gagattcgca ccccgccgct
     2281 gccggcactc ggtgcggcgg ctctgggcac ggcaccgttc atcctgccgg aggtccccgt
     2341 gcgcgtcagt cagtcgtgga cgttcgatcg cgtcacggac gagacgccct cgctaccggt
     2401 cctcgcgctg caggcgcatc tgctggccta ttacgaggag ttcgcgcgat tgctcgaacg
     2461 tgcgcggccc ggatcgacga gccagtggcc ggcgacgggc gcggccgtgc aggaacaacg
     2521 cagtctcgag cgcacggtcg gggcgctgcg cagaacgttg cgcggtgacg tcgcattgcg
     2581 ctccgcggcg ctcgagcagc tccgtggcgg caactatccg atcgcgacgc agaacgcatt
     2641 gctggcgctg ctcgatctct attccggcac gagcagccag ttgctcaact tttacggcgc
     2701 cgcccgcgcg atccataccg tgccctacga tcaggcactg aagggaacgg acggcatcga
     2761 ctggacgggt aaggtcgttt tggtcggtat gtcggagccc gtgcagccgc ggcagcagga
     2821 cgattttata tccgtgtttt cgcagaacac cggaatcaat ttgagcggtg tcgaggtcgg
     2881 ggccacgcgg ctcgcaaatt tgctgcaacg gcatacgctg cgaacgctgc cgttgtcgca
     2941 acaggtcgcg ctcgtcgtcg ttctgggagt cgtcctcggc gcactcgtcg ggcggctcac
     3001 catgttgcgc gcgagcgcgg tcgtcgcgat cggcgcggct ctgtatttcg ccgccgcgta
     3061 ctggcagttc tcgagccatt acgtttggct accgctcgtc gtgccgttgc tcttgcagct
     3121 gccgcttggt ttcggcgccg ccgtttggtg gaactatcgc gacgtctcgc agcagcgcga
     3181 gcgcgtgcag actgcactcg gttactacgt gccgccgtcg ctcgcacggc ggctcagcga
     3241 gcaaaccgtg tcggccggca ccaatcgcga gctgttgcac ggcacgtgcc tcgtgaccga
     3301 cgcagagcac tacacctcga tcgcagagcg cttgtcgccc gccgagcttg ctgtgctgat
     3361 gaacgactac tacgagtcgg tcttccgcgt cgtgcaagcg tacggcggcg agatctcgga
     3421 tacggcgggc gattcgatga tcgccgtgtg ggccagcgcg gagccggacg cggccgtgcg
     3481 gttacgtgcg gcagaagcgt cgctcgcaat tctcgaaacg gttgcggcgt tcaatcgcga
     3541 gcatccgcag acgccgttgc cgacgcgcat cggtttggaa tccggagaga tgttgctcgg
     3601 caatatcggc gccgagcaac gttacgagta ccgcgcgatc ggcgacatcg tcaacaccgc
     3661 ggcgcggatt caaggcttga accagccgct cggcacacgc gtcttgatct cggctgcgac
     3721 gctcgacgag caggcgcggc tcccgtcgcg tgatgtcggc acgtttttat tgcgcggtaa
     3781 gcgcctgccc gtgcgcgtcg tcgagcctct gagcgcggcg acttgcgcgc tcggtgacga
     3841 ggagctcagc aaattcgcgg ccgctctcgc ggcgttcaga gcggggagtt ggcgagaggc
     3901 gcacgaacgg ttcgcgccgc tggccgagcg ttttttcgac gacggaccga gccgctatta
     3961 cgacgcgacg gcccgcgcgt tcctaacgga gccgccggcg agttggacgg gcgctattcg
     4021 gctcacgtcc aaatagcggc tcacaaatag cggctcaaga aggcctgacc ctctcggtcg
     4081 ccgcgaagtc gaagcgcgtc actgaatcgc gttgttgccc gctcgaggct gcgttgcgct
     4141 cgagataagc gagcacgtcg tcgacgcgcg cgacgcactc gcgttttgta tttggttgct
     4201 gttccagcaa ctcccgcagc atgcgctgtg tccggttttg aaccgtcagc ggaacgagag
     4261 tgagcaagtc gaccatgcgg agaattttgt cctgatcctc gcttggcaag cgctcgacgg
     4321 tgcgcacgag tcgcagaacg tcgtctgata cgggcataac ggcttcccct ttcgtctgag
     4381 cccaatgaca gtgttcatgt ctatcaaagg cagaagcacg cgtctgtcag gtaccggaca
     4441 cttcacacga aataaaacta aatgatgagt tgtgtcggtt agttgacaga atcccgattc
     4501 tgaagctggg acgcttactc ccggatgcgc tgcatccgtg ctgttggtag caactttagg
     4561 agtaagcgaa agtgacgaag gtacgcaatc tgcttttgat cgcgttggtg atgatgcccg
     4621 ccgtgacgtt gaacgccagc tcgctgaaca acgccgaacc gcgacccggt gttggtgcgc
     4681 cgctgcaggt cacggggtcg tgctgggtct acatgaacgg ccgttggtat ctgttgccct
     4741 gctgagctac gtcaaacgct gttcgtctcc gcaatcaatc cgcgcaatgc ttcggtgttg
     4801 cggatgacga tctgcgttcg gccgagctcg atccagccgt tacggcgcca atcggccaag
     4861 tgctgattga cgatctggcg cgagatgccg aggaagcgcg ccagctcgcc ctgcgagatc
     4921 cgtagctcga ggtgaccgac gcgcgcggcg cggccgtgca acgacgcaag aatcagcagt
     4981 cgtttcgcga gccgcgccgg tcctgccatg aaagccgatt cttcgacgag ctcgctcgtc
     5041 cagcgcagcc gttcgcacat caatttcagc aggtggatcg cgagctgcgg ctcgcgctcc
     5101 agaaacggca ggaagtcggt gcgttggatc acgatcaacg tcgacgtgtc gagcgacacg
     5161 gcgttggcag ttcgcggcaa cccatccatg acggcgatct cgccgatcgt gtcgccgggc
     5221 tccatgatgt tcaagaacac ttcgcgcccg cccgacgcgc tcgcgctgat gcgcacgcgg
     5281 cccgatgcga cgccgaataa tgaatcgccc acgccgccct gcgagaaaat cacactgctc
     5341 ttgtaatagg tgcgcttggc cgcgagcgca gcgatgcctt cgagcgtggc gtcggaaaga
     5401 ccgcgaaaca aatagttgtg tcgcagaacg gccttcgggc tcagatgcgc cggtgcgggt
     5461 gagccgtcgt cgctgtcgtt gatctggcgc atagcgaagc cttcgtaggg gcgtcggcgg
     5521 cgattcgttg ccgccacgtt tgccgctctt gtttcggccc agtatcgtgt actggctaag
     5581 caattgccaa gcggcgcgca ccagcggagt ggagcggtga tcaagacccc atggcggctt
     5641 gccggccgtt ttctggccag ggactggcga tcgggcgaag tgctcgtgct gctggctgcg
     5701 ctcgtggtcg cggtcgcggc catgagcgcc gtcacctttt ttaccgaccg cgtgcgagcg
     5761 gcggtgtcgc agcaagccgg cgaggcgttg gctgcggacc tacgtgtcga atcgatcaat
     5821 ccgttacctg ccgaaattcg cgaagcggcg acgcggcgcg ggctcgcgac tgcagacgtc
     5881 gtgagcttcc gcagcgtcgt cgcggccggc gagacgactt ccctggccga cgttcgcggt
     5941 gtgacggccg gttacccgct gcgcggcgtc gtgcaggttg ccgatcgatt ggccggcgtg
     6001 cccgaagacg cgagcggcgt tccgcctcgc ggcgaggtct gggctgagcc cagcctgatg
     6061 gcgcggctcg gtaccgcagt aggcgacgac ctcgagatcg gccagctccg actcaagatc
     6121 acgcgaacgc tcgagttccg acccgacgaa ggctggcgcc tgatggagat cgcgccgacc
     6181 gtgctgctga gctacgacga cgtgttggcg tcgggtttgc tcgcgccggg aagcatcgct
     6241 caatacgtgg ggctctacgc cggcgaggcg gccgatgtcg aggcgtttcg ccgcgagctg
     6301 ccgtcgttgc tgcgacctca agaccggatc gaggacttcc gcaacggtcg gcccgaagtc
     6361 ggcgccgcca tcgccaacgc ggagcgattc ctcgtgctgg ccgcgctcgt cagcgtgctg
     6421 ctcggcggcg tcgcggtcgc catggcggcg aggcgtttcg ttgcgcgccg gctcgatgcc
     6481 gtggcgctca tgaagtgcct cggtgcccgg catcgcgagg tgctgcagtt aaacctgctg
     6541 caactcttga tactggtcgt ggccgccggc gtgctcggtt gtttgattgg cttggctgcg
     6601 cagtttggtt tgacggccct gctcgccgac ttcatcgaag cgcaacttcc gccgccgagc
     6661 ctgcaaggcg ccgtgttggg cccgttgacg gccttggccg tcgcgatcgg ctgcgcgttg
     6721 ccgccgctgc tgcagctcgg cggcgtgccg cctgctcgcg tgctgcgcaa tgacgttggg
     6781 ccgccgccgc tgcgctacac gacgatctac ggcgtcgccg cggtagccgt cacggcgctg
     6841 atgtacgtgc tgttcggcga cgtcgagctc atcgcctatc tgctcggcgg tgcgatcgcg
     6901 acgttcttcg tgctgtacct cgcgggtcgc ttgctcgtgc tcgcgttgca gcgcctgcgt
     6961 ggcggagtcg gcgtcgcgtg gcggtacggc attgcgaacg tcgcgcggcg cggcagggaa
     7021 agcagcgtcc aggtcgtggc gttcgggatc ggattgatgg tgctgctgct gctgacgtcc
     7081 gtgcgcacgg agctcatggc cgaatggcag gccacactgc ccgccaatgc gcccaatcac
     7141 tttctcatta atatccagcc tgcggagcgc gcggcgctcg cggcgacgtt ggtggcggcg
     7201 ggcgttccgg agccggaaat gacgccgctc gtgcgagctc gaatgagcca catcaacggt
     7261 gtgccggtcg agcaatatca ggcggcgacc gacttcggcc gccgcgagct cgatgacgaa
     7321 acgaacttga cgtggtcggc cgacttgccg cccgacaacg agcttgcgtc gggcgcatgg
     7381 tggggcggaa acccggacgt gccgcaggtc tcgctcgagg aaggcttgag ggaaggaatc
     7441 gggttgaagc tcggcgacga gatcacctac gcgatcggcg gcgagagtct caccgtgcgc
     7501 ttgacgagca cgcgcacggt gcactgggaa tcgtttcgcc cgaacttctt catggttctt
     7561 agccccggcg ccatcgaagg attcgcgcac acctacatta cgagcctgta cctgctgccc
     7621 gagcagcggc gcgtgaccgt cgatctcgtc cggcagtacc cgagcgtgtc ggtcttggac
     7681 attggcgccg tgatcgagca ggtgcgaagg tcgatggacc gcgcggcgct ggccgtgcag
     7741 tacgtgttct tgttcacgct ggcggcgggc gtcatggtcc tgctggccgc gattcaagcg
     7801 acgcgcgacg aacgcaggtt cgagagcgca g
//
LOCUS       DATLEE010000005         2026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3859, whole genome shotgun sequence.
ACCESSION   DATLEE010000005 DATLEE010000000
VERSION     DATLEE010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3859"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..830
                     /locus_tag="VM692_00140"
     CDS             138..830
                     /locus_tag="VM692_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50593.1"
                     /translation="MPHDRQLETLLRPLLEARPAFSRRSFIARGTAAAATIALPTAAR
                     ADIDYSQFRQVPTQYIAALAPPDATSGENAETWGLWRQDPGPRGVRLANLASLKNGVA
                     PAGWQHDQNAWWLEEHGLLMEAPDFPMPAGVYLVTGDRAVTSVLTVQAPGTNGRQRWE
                     LANGATLYDVTHLRCRSAVYTPAAAGGSCSPTAARQSDFPVAPGAAMPPVANCAKQDY
                     QVLIVIGKAKDA"
     gene            complement(871..>2026)
                     /locus_tag="VM692_00145"
     CDS             complement(871..>2026)
                     /locus_tag="VM692_00145"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HUQ50594.1"
                     /translation="GYATAASDVGHQGSPLDGMFLTGHPEKLTDFGHRAVHESTVAAK
                     AAIAKFYGESPQRSLYASCSTGGRIGLMEAYRYPEDYDAISAIAPANPMVPLMAMSLW
                     SGNATMKDEASRIPAPKYALAQQAALQACDADDGVRDGIIGNPPTCKFDPAMLQCKSG
                     DAPDCLTAPQVQALRDVYRGPRNPRTGEQIYPGLSIGAEAQMQVLTMGQEPFPVATTF
                     FRGPVFNDPTWNFRAFDYDRDLERAKAYGSDALDVPPTGLDRFIAGNRKLLLSHGWSD
                     GLIPAQSTIDFYDALVKDVGARRARDNVRLFMVPGMTHCAGGDGPSAIDFLGTIERWV
                     ETGQAPERLVAANPPNAPKRTRPLCPYPQVAKYTGTGSTDEEQNFRCEGP"
BASE COUNT          311 a          700 c          680 g          335 t
ORIGIN      
        1 ggcaaccgtt gtggacggcg ttttcatgat gtcaatcgta cacgcgcgcg tgcgcggctc
       61 ttcggcgact gcgcgttttt tgggcagagc gggcgtatga tcggagtcat tcacgccgca
      121 gcacgggaga ccttgcgatg ccgcacgacc gtcaactgga aaccctgctt cgcccgctgc
      181 tcgaagcgcg ccccgccttc agccgccgct cgttcatcgc tcgcggcact gccgccgccg
      241 cgacgatcgc gctgccgacc gcggcgcggg ccgacatcga ctacagccag ttcaggcaag
      301 tgccgacgca gtacatcgcg gctctcgcgc cgccggacgc cacctccggc gagaacgccg
      361 agacctgggg cctgtggcgt caagatcctg gcccgcgcgg tgtgcggctc gcgaatctcg
      421 caagcctcaa gaacggcgtc gcacccgccg gttggcagca cgaccaaaac gcctggtggc
      481 tcgaggagca cggcttactc atggaagcgc cggactttcc catgccggcc ggcgtctacc
      541 tcgtgaccgg cgatcgcgca gtcacgagcg tcctcacggt gcaggcgccg ggcacgaacg
      601 gacggcaacg ctgggagctc gcgaacggcg caacgctcta cgacgtgacg cacttgcgct
      661 gccgctcggc ggtctacacg ccggcggcgg ccggcgggtc gtgctcaccg acggcggcgc
      721 ggcaaagcga cttccccgtg gcgcccggcg cggccatgcc gcccgtagcc aactgcgcga
      781 agcaggacta ccaagtgctg atcgtgatcg ggaaagcgaa ggacgcctga ggccgcgccg
      841 gctccgtctt taccgccccg tttgctgcgc ctacggcccc tcgcagcgga aattctgctc
      901 ctcgtccgtg ctgcccgtgc ccgtgtactt cgcgacttgc ggatacgggc acagcggtcg
      961 cgttcgcttt ggggcattcg gcggattcgc ggcgacgagc cgctccggag cctggccggt
     1021 ctcgacccaa cgctcgatcg tgccgaggaa atcgatcgcc gacggcccgt cacctccggc
     1081 acagtgcgtc atgcccggca ccatgaagag ccgtacgttg tcgcgcgcgc ggcgcgcgcc
     1141 gacatctttg acgagcgcat cgtagaaatc gatcgtcgat tgcgcgggga tgagaccgtc
     1201 cgaccagccg tgcgagagca gcagcttgcg gttgcctgcg atgaaacggt cgaggcccgt
     1261 cggcggcacg tcgagcgcat cgctgccgta cgctttcgcg cgctcgagat cgcgatcgta
     1321 gtcgaaggca cggaagttcc aggtcggatc gttgaagacc ggcccgcgga aaaacgtcgt
     1381 cgcgacggga aacggctcct gccccatcgt cagcacctgc atctgcgcct ctgcgccgat
     1441 cgataagccg gggtaaattt gctcgcccgt gcgcggattg cgcgggcctc gatagacgtc
     1501 acgcagcgcc tgcacctgcg gcgccgtcaa gcagtcaggc gcgtcgccgc ttttgcactg
     1561 cagcatcgca ggatcgaact tgcatgtcgg ggggttgccg atgatgccgt cgcgcacgcc
     1621 gtcgtcggcg tcgcaagcct gcagcgcggc ctgctgcgcg agcgcgtatt tcggcgccgg
     1681 gatccggctc gcctcgtcct tcatcgtcgc gttcccgctc cacaacgaca tcgccatcag
     1741 cggcaccatc ggattcgccg gcgcgatggc cgagatggcg tcgtagtcct cggggtagcg
     1801 gtacgcttcc atgaggccga tgcggccgcc cgtcgagcag ctcgcgtaca gcgaacgttg
     1861 cgggctctcg ccgtagaact ttgcgatggc ggctttggcg gcgaccgtgg actcgtgcac
     1921 ggcgcggtgg ccgaagtccg tgagcttctc cgggtgcccc gtcagaaaca tgccgtcgag
     1981 ggggctgcct tggtgaccga cgtccgaagc ggccgtcgcg tagccg
//
LOCUS       DATLEE010000006         6301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8946560, whole genome shotgun sequence.
ACCESSION   DATLEE010000006 DATLEE010000000
VERSION     DATLEE010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8946560"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VM692_00150"
     CDS             complement(<1..656)
                     /locus_tag="VM692_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HUQ50595.1"
                     /translation="MPAAENDQQPSGFFGKLKARLNRGTNWLASELLGLGGEPFDERT
                     VEELETRLLTADVGVDATTWLVDEMRSMNQRNPGAPAVELLRQTATALLRTVEAPLAI
                     APKHKPFVILAVGVNGTGKTTTVGKLAHRLKARGFSVLLAAADTFRAAAVEQLQEWGE
                     RAQVPVVSQDAGADPAAVVHDALVAARARDVDVVLADTAGRLHTAAGLMDELEKIKRV
                     "
     gene            701..1309
                     /gene="rsmD"
                     /locus_tag="VM692_00155"
     CDS             701..1309
                     /gene="rsmD"
                     /locus_tag="VM692_00155"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HUQ50596.1"
                     /translation="MVKRNAGRVQGSVRIIAGEWRGRRIQIPDGTSVRPTPDRVRETV
                     FNWLRDSLTGARCLDLYAGTGVLGFEALSRGAAEAWLIERDAALVAALQSTALKLGAS
                     PKVVRREALAFLREPAGARFDVVFLDPPYAQPLDALLELLPRWLTPQASVYVERPRSE
                     GLPAVAGAQWLKQSHAAAVEYGLLRFVEATMPQTPPSEARPP"
     gene            1321..1791
                     /gene="coaD"
                     /locus_tag="VM692_00160"
     CDS             1321..1791
                     /gene="coaD"
                     /locus_tag="VM692_00160"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HUQ50597.1"
                     /translation="MYPGTFDPITRGHEDLVRRCVRLFDQVVIAIASNPKKTPLFSLD
                     ERVQLAREVLRDLPEVSVTGYTGLTVDFARDHGFKAIIRGLRAVSDFEYEFQLATMNR
                     HLIEGVETVFLTPSEHHTFISSSLVREIAEFGGDVGAFVHPVVAERLKQKLGKR"
     gene            1791..3536
                     /gene="ggt"
                     /locus_tag="VM692_00165"
     CDS             1791..3536
                     /gene="ggt"
                     /locus_tag="VM692_00165"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HUQ50598.1"
                     /translation="MLSSSLPRLRCFVFAALVSLSGGAALAQPTAPVRASKQLVVSAN
                     PHASEAGRAILRAGGGAVDAAIAVQLVLTLVEPQSSGIGGGAFMMFYEAPDDGGEGRI
                     TAYEGRETAPAAATPDMFLGANGRPEPFNNVGVGGLSVGVPGALRMLEAAHREHGRLP
                     WEQLFAPAIELAENGFEVSPRLNGLLNGFKRFARGDDFRSYFYDASGEPHPVGHLLKN
                     PEYAAALKLLAARGAEPMHTGELAAAIAAEVRDNNVRPGRMTPEDLAAYEPHMSAPLC
                     TAYRSWRVCGPQLPSSGGVTTQQVLGMLAHFDLPDIRTQPAQAIHLFAEANRLAFADR
                     NFYLGDPQFVPAPVAGLLDPSYLRNRAALIDPTKALATISAGEPLPAAAWDYAPGTAT
                     ERPSTSHFSIVDRFGDGVAMTTSVQGAFGSQLMVGGFILNNQLTDFDYVPSVGGKPVA
                     NRIEGGKRPLSSMSPTMLLDERGRLRLLVGSPGGTRIIGFVAQSIIGVVDWNLDVQQA
                     VAAPHFLAEEGPVELEEGTAITANEEALEALGHNVALNDLNSGLHAIAVEHTRRGRVL
                     YGGVDPRREGAALGD"
     gene            complement(3533..4345)
                     /gene="mutM"
                     /locus_tag="VM692_00170"
     CDS             complement(3533..4345)
                     /gene="mutM"
                     /locus_tag="VM692_00170"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HUQ50599.1"
                     /translation="MPELPEVETTRRGLLPHVVGRAIARVEVREPRLRWPVARQLPRA
                     LAATRIDSLERRGKYLLFGTAAGTLLVHLGMSGSLRYLPAPAAPGLHDHIDVHFEDGG
                     ALRFNDPRRFGSFMLTSTPSSHPLLKSLGPEPLGDDFDADYLWGTSRNRRVAIKQHVM
                     NGRVVVGVGNIYASEALFRAGIHPRRIAGRISRARFEPLVSSIRDVLRDAIDEGGTTL
                     RNFVGGDGKPGYFRGSLRVYERDGSPCVNCGTAIERRVMGQRATYYCPRCQR"
     gene            4518..5732
                     /locus_tag="VM692_00175"
     CDS             4518..5732
                     /locus_tag="VM692_00175"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HUQ50600.1"
                     /translation="MNFLFEHFYGLANLSLLGYIAVTVLLLHVTLIAVTLYYHRDQAH
                     RSVDLHPAVRHFCRFWLWLNTGSSTREWVAVHRRHHAHCEKEGDPHSPRLFGLKKVVL
                     EGAELYRAAARDPDTIEKYAKGTPNDWLENNLYHHPTRSYYGIAFLVVANLVLFGVPG
                     IIMIALQLVNMPFLAAGIVNGVAHAKGYRNFETDDGATNLWPIGIVIAGEELHNNHHA
                     FPTSARFSMRPHEVDMGWLHLKVLVWLGLAKVRRVANPPQLEATSRATTELDDLRAII
                     VHRMHVLRAYTYNVTLPVLRRELESLGENANSLLRATRRHLSWHPDMLDESSRQRLDE
                     LAQRHPRFKQVLEFRRELKQLWDGAHTSNERLLAEFREWCTRAEQSGIQGLEEFVAYL
                     KSFRAMREPAHG"
     gene            complement(5799..5954)
                     /gene="rpmG"
                     /locus_tag="VM692_00180"
     CDS             complement(5799..5954)
                     /gene="rpmG"
                     /locus_tag="VM692_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HUQ50601.1"
                     /translation="MREKIRLMSSAGTGHYYTTTKNRRLHPEKMEVMKYDPVVRKHVA
                     YKETKIK"
     gene            complement(5969..6205)
                     /gene="rpmB"
                     /locus_tag="VM692_00185"
     CDS             complement(5969..6205)
                     /gene="rpmB"
                     /locus_tag="VM692_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HUQ50602.1"
                     /translation="MSRVCQVTGKGPMVGNKVSHANNKTKRRFLPNLHSQRFWLEGEK
                     RFVRLRVSRQGLRIIDKKGIEKVVAELRAKGEHI"
BASE COUNT         1042 a         2062 c         2072 g         1125 t
ORIGIN      
        1 atcacgcgtt tgatcttctc gagctcgtcc atgagaccgg ccgcagtgtg aaggcgaccg
       61 gcggtgtccg ccaatacgac gtcgacgtcg cgcgctcggg ccgcgacgag agcatcgtga
      121 acgacggcag ccggatcggc cccggcatct tgcgagacga cgggcacctg cgcgcgctcg
      181 ccccattctt gcagctgctc gacggcggcc gcgcggaacg tgtcggcggc cgcgagcagc
      241 acgctgaagc ccctcgcttt caaacgatgc gcaagcttgc cgacggtcgt cgtcttgccc
      301 gtaccgttaa cgcccacagc caagatcacg aacggcttgt gcttcggcgc gattgcgagc
      361 ggcgcctcga cggtccgcag caacgcggtc gcagtctgcc gcagcagctc gacggctggt
      421 gcgccggggt tgcgctgatt catggaccgc atctcgtcga cgagccaagt cgtcgcgtcg
      481 acgccgacgt cggcggtcag caggcgcgtc tcgagctctt cgacggttcg ctcgtcgaac
      541 ggctcgccgc ctagcccgag cagctccgac gcgagccaat tcgtgccgcg gttcaaacgc
      601 gccttcagct tgccgaagaa tccggacggc tgttgatcgt tctcggcggc gggcatgcgg
      661 tgaaaaattc tcgcggtgag cttctgctag tgtgatgcac atggtaaagc gcaacgccgg
      721 gcgtgtgcag ggtagcgtcc gcatcatcgc cggcgagtgg cgcgggaggc gcatccagat
      781 tcccgacggc acgagcgttc gcccgacgcc cgatcgagtg cgcgagacag tcttcaattg
      841 gttacgagac tcgttgaccg gcgcccgctg tctcgatttg tacgcgggaa cgggagtgtt
      901 gggtttcgag gcgctgtcgc gcggcgctgc cgaagcttgg ctcatcgagc gcgatgccgc
      961 gctcgtcgcc gctctgcagt cgacggcact taagctcggc gcctcgccga aggtcgtgcg
     1021 ccgagaagcg ctcgccttcc tgcgcgagcc ggccggagct cggttcgacg tggtgttcct
     1081 cgatccacca tacgcgcagc cgctcgatgc gttgctcgag ctgttgcctc gctggttgac
     1141 gccacaggcc tcggtgtacg tcgagcggcc gcgcagcgag ggtctacccg cagtagccgg
     1201 cgcgcagtgg ctcaagcaga gccatgcggc ggccgtcgag tacggactcc tgcggttcgt
     1261 tgaggctaca atgccgcaga ctcccccgag cgaagcgcgg ccaccgtgaa aatcggcgca
     1321 atgtatcccg gcacgttcga cccgatcact cgcggtcacg aggatttggt tcggcgctgc
     1381 gtgcggctgt tcgaccaggt cgtcatcgca atcgcgtcga acccgaaaaa gacgccgctg
     1441 ttctcgctcg acgagcgcgt ccagctcgcg cgagaggtct tgcgcgatct gcccgaagtg
     1501 tccgttacgg gctacacggg gctcaccgtc gacttcgcgc gcgatcacgg tttcaaggcg
     1561 atcattcgcg ggctgcgcgc cgtctccgac ttcgagtacg agtttcagct cgcgacgatg
     1621 aatcgacatt tgatcgaggg agtcgagacc gttttcttga cgccgtcgga gcaccacacg
     1681 ttcatctcgt cgagcttggt gcgcgagatc gcggagttcg gcggcgacgt cggcgcgttc
     1741 gttcatcctg tcgtcgctga gcgattgaag cagaagcttg gaaagcggtg atgctgagct
     1801 cgagcttgcc gcggttgcgc tgtttcgtat tcgcagcgct cgtctcgctc tcgggcggcg
     1861 cagcgctggc acagccgacc gcgcccgtgc gagcgagcaa gcagctcgtc gtctcggcga
     1921 atccgcatgc ctccgaagcg gggcgagcga tcttgcgtgc cggcggcggc gccgtcgatg
     1981 ctgctatagc cgtgcagctc gtgctgacgc tcgtcgagcc ccaatcgtcg gggatcggcg
     2041 gcggcgcgtt catgatgttc tacgaggcgc ccgacgatgg cggcgaaggc cgcatcacgg
     2101 cgtacgaagg ccgcgagacg gccccggccg ctgcaacgcc ggacatgttc ctcggcgcca
     2161 acgggcgccc ggagccgttc aataacgtcg gcgtcggcgg gctctccgtg ggcgtgccgg
     2221 gcgcgctgcg catgctcgaa gcggcgcatc gcgagcacgg gcgtttgccg tgggagcagc
     2281 tgttcgcgcc ggcgatcgag ctcgcggaga acggcttcga ggtgtcgccg cgcctcaacg
     2341 gcttgttgaa cggcttcaag cgcttcgcgc gtggcgacga tttccgcagc tatttctacg
     2401 acgcgagcgg cgagcctcac cccgtcggtc acctgctcaa gaaccccgag tatgcggcgg
     2461 cgctgaagct gctcgcggcg cgcggtgcgg agcctatgca cacgggcgag ctcgcggctg
     2521 cgatcgccgc cgaggtccgc gacaacaacg ttcgcccggg caggatgacg ccggaggacc
     2581 tcgctgcgta cgagccgcac atgtcggcgc cgctgtgcac ggcgtaccgc tcgtggcgcg
     2641 tctgcgggcc gcagttgcca tcgtcgggcg gcgtgacgac gcagcaagtg ctcggcatgc
     2701 tcgcgcactt cgacttgccg gacattcgga cgcagccggc gcaggcgatt cacttgttcg
     2761 ccgaggccaa ccgactcgcg ttcgccgatc gcaacttcta tctcggcgat ccgcaattcg
     2821 tgccggctcc cgtcgcgggg ctgctcgatc cgagctacct gcgcaatcgc gccgcgctga
     2881 tcgatcccac caaggctctc gcgacgatct ccgccggcga gccattgccg gcggccgctt
     2941 gggactacgc gccgggcact gcgacggaac ggccgtcgac gagccacttc tcgatcgtcg
     3001 accgctttgg cgacggcgtt gcgatgacga cgagcgtcca aggcgccttc ggcagtcagc
     3061 tcatggtcgg cggcttcatc ttgaacaacc agctgacgga cttcgactac gttccgtcgg
     3121 tgggcggtaa gcccgtcgcg aatcgcatcg agggcggcaa gcgaccgctg tcgagcatgt
     3181 cgccgacgat gctgctcgac gagcgcgggc gtctgcgctt gctcgtcggc tcgccgggcg
     3241 gcacgcgcat catcgggttc gtcgcgcaga gcatcatcgg cgtcgtcgac tggaacctcg
     3301 atgtgcagca agccgtcgcg gcgccgcact tcctcgccga ggaagggccg gtcgagctcg
     3361 aggaaggaac cgcgatcacg gccaacgaag aagcgctcga ggcgctcggg cacaacgtag
     3421 cgctgaacga cttgaacagc ggcttgcacg caatcgccgt cgagcacacg cgccgcggcc
     3481 gcgtgctcta cggcggcgtc gatccgcgcc gcgaaggtgc cgcgctcggc gactaacgct
     3541 ggcagcgcgg gcagtaatac gtcgcgcgct ggcccatgac ccgccgctcg atcgcggtgc
     3601 cgcaattcac acacggcgat ccgtcacgct cgtaaacgcg cagcgagccg cggaagtagc
     3661 ccggcttgcc gtcgccgcca acgaaattac gcaacgtcgt gccgccttcg tcgatggcgt
     3721 cgcgcaacac atcgcggatc gagctcacga gcggctcgaa acgcgctcgc gatattcgac
     3781 ctgcgatgcg tcgtggatgg atgcccgcgc ggaacagcgc ctcgctcgca tagatgttgc
     3841 cgacgccgac gacgacgcgg ccgttcatca cgtgttgctt gatcgcgacg cggcggttgc
     3901 gactcgtgcc ccacagatag tcggcatcga agtcgtcgcc gagcggctcg gggcccaagc
     3961 tcttgagcag cgggtgcgag gagggtgtcg acgtcaacat gaagctgccg aaacgccgcg
     4021 ggtcgttgaa ccttagggcg ccgccgtcct cgaagtgtac gtcgatgtga tcgtgcaagc
     4081 ccggcgcggc cggcgccggc agataacgca ggctgccgga catgccgagg tgcacgagca
     4141 gcgtgccggc cgccgtgccg aatagcaggt atttgccgcg ccgctcgagg ctgtcgatgc
     4201 gcgtggccgc cagcgcacgc ggcagttgcc tggccaccgg ccagcgcagg cggggctcgc
     4261 ggacctcgac gcgagcgatc gcacggccca cgacgtgcgg cagtaggccg cggcgcgtag
     4321 tctccacctc gggcagttcg ggcatcgcgc gagcttacgc cgcacgccgg cgccgcgaaa
     4381 tcttaagtct tcgtgacgct tctcacgctg aggggtgggt cgatggagta ggttcggtca
     4441 atacgctaaa attgaatggc aacataacgg cccgacgtag tttcagtcgt gccgacattt
     4501 acaaggactc gtaagccgtg aacttcctgt tcgagcactt ctacggtctc gccaatctga
     4561 gcctgctcgg ctacatcgcc gtcacggttc tcttgctgca cgtgacgctg attgcggtaa
     4621 ccctctacta ccatcgcgat caagcgcacc gctccgtaga cctgcatccg gcggtccggc
     4681 acttctgccg cttctggctg tggttgaaca ccggctcgtc gacgcgcgag tgggtagccg
     4741 tgcatcgcag gcatcacgct cactgcgaga aagaaggcga tccgcatagc ccgcgcttgt
     4801 tcggcttgaa gaaggtcgtg ctcgaaggcg ccgagctgta tcgcgctgct gcgcgagatc
     4861 cggacacgat cgagaagtac gcgaagggca cgccgaacga ctggctcgag aacaaccttt
     4921 accaccatcc gacgcgcagc tactacggca tcgcgttcct cgtcgtcgcg aacctcgtgt
     4981 tgttcggtgt gccgggcatc atcatgatcg cgctgcagct cgtgaacatg ccgttcctgg
     5041 ctgcgggcat cgtcaacggc gttgctcacg ccaagggcta tcgcaacttc gagaccgacg
     5101 acggggccac caacctttgg ccgatcggca tcgtgattgc cggcgaagag ctgcacaaca
     5161 accatcacgc gtttccgacg tcggcgcgct tctcgatgcg gccccacgag gtcgacatgg
     5221 gttggctgca cctgaaggtg ctcgtgtggc tcggcctcgc gaaggttcgc cgcgtcgcca
     5281 atccgccgca gctcgaggcc acgtcgcgtg ccacgacgga gctcgacgac ctgcgcgcga
     5341 tcatcgtgca tcgaatgcac gtgctgcgcg cttatacgta caacgtgacg ttgccggtgc
     5401 tgcgccgcga gctcgagagc ttgggcgaga acgcgaactc gttgctgcgg gccacgcgcc
     5461 ggcacttgag ttggcatcct gatatgctcg acgagtcgtc gcgccaacgg ctcgacgagc
     5521 tggcccagcg gcatccgcgg ttcaagcaag tcctcgagtt ccgcagggag ctcaagcagc
     5581 tgtgggatgg cgcgcacacg agcaacgagc gcctgctggc cgagttccgc gaatggtgca
     5641 cgcgcgccga gcagagcggc atccaggggc tcgaggagtt cgtggcctac ctgaagtcat
     5701 tccgcgcgat gcgggagccc gcacacggct agaccaccat tatcccgagc acaaaaaacc
     5761 cgcctcgaag gcgggttttt tttcgatccg tagcagcgct acttgatctt ggtttccttg
     5821 tacgccacgt gcttgcggac gacggggtcg tatttcatga cctccatctt ctcgggatgc
     5881 agccgacggt tcttcgtcgt cgtgtagtag tggcccgtgc cggcagaaga catcaaacga
     5941 attttctcgc gcatggttca gctcgcaatt agatgtgttc gcccttggcg cgcagctcgg
     6001 caacgacttt ctcgatgccc ttcttgtcga tgattcgcag gccctgacgc gagacgcgca
     6061 gacgcacgaa gcgcttttcg ccctcgagcc aaaagcgctg cgagtgcaga ttcggcagga
     6121 aacgacgctt cgtcttgttg ttcgcgtgcg aaaccttgtt gccgaccatc ggccctttgc
     6181 cggtgacttg gcacaccctg gacatggcaa aaattcctga aagcccttga aggggcgggg
     6241 actatacctg ctgacctcgg ggtcgggcaa ggcgcgcaac cgcactcggc cactcgctta
     6301 a
//
LOCUS       DATLEE010000007         2651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2241208, whole genome shotgun sequence.
ACCESSION   DATLEE010000007 DATLEE010000000
VERSION     DATLEE010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241208"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..1364)
                     /locus_tag="VM692_00190"
     CDS             complement(411..1364)
                     /locus_tag="VM692_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009801685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HUQ50603.1"
                     /translation="MQHSHAHTRDDHDHDHGHDHHGRAHGHGHSVNERSVGVAALLTG
                     GFMVAEIAGGLFSGSLALLADAGHMLTDFASLALAWLGFRLTRRPADWRRTYGFDRFA
                     VLVAFVNGISLFAIAVWIVVEAVQRLNSPVAVLGAPMLWIAAAGLAVNVLSFFVLRSG
                     DKDNLNIRAAVLHVIGDLLGSVAAVIAALVILATGWTPIDPLLSIVVALIVIRSAWRV
                     VADSGHILLEGSPPGVDSRTLRDHLRSTLPFVLDIHHVHAWSISQERPMVTLHASIAA
                     STDSAVAVREIKRQLALHFRITHATIEIEYDACGDEPSSAC"
     gene            1496..>2651
                     /locus_tag="VM692_00195"
     CDS             1496..>2651
                     /locus_tag="VM692_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUQ50604.1"
                     /translation="MALKARQKIGKYRIERRISEGPIAAVYEAVDTIHGLHVALKIPH
                     ESSMSEHFLVDFKREARLAHRLEHPNILPIRDASFIADRFVIAMPLGERSLASRMRQR
                     LATDSALELIEQALAALAHAHSANVIHCDIKPDNFILFPGNRLKLTDFGFSKVAEHTL
                     RASGSGTVGYIAPEQAVGRPKFQSDVFSLGLLIYELLSGHLPEWPYDWPPPQVKRVRE
                     KLGANLMGWLRRSIEVRPEDRFKNAATMYREFKRLRNGAGKRQRNRERKADDPSLWQT
                     VLFRQFSRKYGKSIEARYRCRHCTGPVSELMQSCPWCGTQPPLDRHRSRHPAECPRCR
                     RGVKTDWHYCPSCYGPGFEVETTRRFSDKRYSTKCQNETCRGPLMPFMRYC"
BASE COUNT          486 a          841 c          900 g          424 t
ORIGIN      
        1 cggcgcgatt tccttctgta ctgagcggca agcctccccg aaatgttcaa cgcgaccatg
       61 ctggagcgca catcgttcgt tgagtgctcg gctgcgatcg cggtgaacga cagcgtggcg
      121 acgacgacgg cactggctaa ccgggacatc tgacaaactc cctatgggtg tgtggacagc
      181 tagtgcaata gcaagcggcg tgccgccgct cgcgaacacg gcgaactcgt tgatttggct
      241 tacaacatcg tttcagcgcg agccgatcgt ctcgcgcggt ggatagtcgg attcgctaac
      301 tattgccggc gcgagccgag cgccggtgcg cgcggatagt agtcagcgag cgaccgcgac
      361 cggttgtgcg gtcgcagctg ccgacgcgtt gccggcacgc cgctgctgca tcagcaggcc
      421 gacgaaggtt cgtcgccgca ggcgtcgtac tcgatctcga tcgtcgcgtg cgtgatgcgg
      481 aagtgcaggg caagctggcg cttgatctcg cggacggcga cggccgagtc ggtgctcgcc
      541 gcgatgctcg cgtgcagagt gaccatcggc cgctcctggc tgatcgacca tgcgtgcacg
      601 tgatggatgt ccaacacgaa cggcaacgtc gagcgcagat gatcgcgcag ggtgcgcgag
      661 tcgacgccgg ggggcgagcc ttcgaggagg atgtggccgc tgtcggcgac gactcgccag
      721 gccgagcgga tgacgatcag cgccacgacg atcgacagca gcgggtcgat gggcgtccaa
      781 cccgtcgcaa ggatcacgag cgcagcgatg acggccgcga ccgagcccaa caggtcgccg
      841 atgacgtgca gcacggccgc gcggatgttc aagttgtctt tgtcgccgga gcgcagcacg
      901 aaaaacgaga gcacgttgac ggcgaggccg gctgccgcga tccacagcat cggcgcgccg
      961 agcacggcga ccggcgaatt caagcgctgc acggcttcga cgacgatcca aacggcgatt
     1021 gcgaataacg agatgccgtt gacgaacgcc acgaggacgg cgaagcggtc gaagccgtag
     1081 gtacgccgcc agtcggccgg ccggcgcgtg agccgaaagc cgagccaagc gagcgcaagg
     1141 ctcgcgaagt ccgtcaacat gtggcccgcg tcggcgagca gagccaggga cccggaaaag
     1201 agcccgccgg cgatttcggc caccatgaac ccgcccgtca acagggcggc gacgccgacg
     1261 ctgcgctcgt tgaccgaatg gccatggccg tgcgcgcgcc catgatggtc atggccatga
     1321 tcatggtcgt ggtcgtcgcg cgtgtgagcg tgggagtgct gcatgggctc gcgcgcgaat
     1381 ggtagcacgc gcgaattgac cgcttcgccg tccgcgggga tcatggctag cccttgagac
     1441 aacgacgagc gtccgatcgc acgggcgccc aagctcgaat agatcggcag acttcgtggc
     1501 gctgaaggca cggcaaaaaa tcggcaagta ccgcatcgag cggcgcatct ccgaaggtcc
     1561 gatcgcggcc gtctacgagg ccgtcgacac gatccacggt ttgcacgtcg cgctgaagat
     1621 tccgcacgag agctcgatga gcgagcactt tctcgtcgac ttcaagcgcg aagccaggct
     1681 cgcgcatcgg ctcgagcatc cgaacatcct gccgatccgc gacgcgtcgt tcatcgcgga
     1741 ccggttcgtg atcgcgatgc cgctcggcga gcgctcgctc gcgagccgca tgcggcagcg
     1801 attggcgacg gactcggcgc tggagctgat cgagcaagcg ctcgcggcct tggcgcacgc
     1861 gcatagcgcg aacgtcattc attgcgacat caagcccgac aacttcatcc tgtttcccgg
     1921 caatcggctg aagctgacgg acttcggttt ttcgaaagtc gcagagcaca cgctgcgggc
     1981 gtccggctcg gggaccgtcg gctacatcgc acccgagcag gccgtgggcc ggccgaagtt
     2041 ccaatccgac gtgttttcgc tcggcctctt gatctacgag ctgttgtcgg ggcacttgcc
     2101 cgagtggccg tacgattggc cgccgccgca ggtcaagcgc gtccgcgaga agctcggcgc
     2161 gaacctcatg ggctggttgc gtcgctcgat cgaggtccgg cccgaggacc gtttcaagaa
     2221 cgcggcgacg atgtatcgcg agttcaagcg gctgcgcaac ggcgccggca agcggcagcg
     2281 gaaccgcgag cggaaagcgg acgacccgtc gctgtggcaa accgtgctgt ttcgccagtt
     2341 ctcgcgcaag tacggtaagt ccatcgaggc gcgctatcgt tgccgccact gcacggggcc
     2401 ggtgtcggag ctcatgcaga gttgcccatg gtgcggcacg cagccgccgc tcgatcgcca
     2461 tcgcagccgg catccggccg agtgcccgcg ttgccgccgc ggcgtcaaaa cggattggca
     2521 ctattgcccg tcgtgctacg gcccaggttt cgaggtcgag acgacgcggc gcttctccga
     2581 caagcgttac agcaccaagt gccagaacga gacgtgccgc gggccgctga tgccgttcat
     2641 gcgctactgt c
//
LOCUS       DATLEE010000008         2338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10436204, whole genome shotgun sequence.
ACCESSION   DATLEE010000008 DATLEE010000000
VERSION     DATLEE010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10436204"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VM692_00200"
     CDS             <1..748
                     /locus_tag="VM692_00200"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HUQ50605.1"
                     /translation="SYMSLTEALRHAGIRTRTRVNILYMESEEIQTQGTSCLNDVDAI
                     LVPGGFGERGIEGKIAAVKFAREKQVPYLGICLGMQVATIEYARNVAGLAGAHSTELN
                     RETPHPVIAMITEWQDRDGSIQERNAASDLGGTMRLGAQTCYVKDGTLAKQIYGADEI
                     SERHRHRYEFNNRYLEQLSKAGLVFSGFSRDDLVELIELPPAVHPWFFATQFHPEFNS
                     NPRDGHPLFTSFISAARACQGLKLPRVASA"
     gene            745..1590
                     /gene="kdsA"
                     /locus_tag="VM692_00205"
     CDS             745..1590
                     /gene="kdsA"
                     /locus_tag="VM692_00205"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HUQ50606.1"
                     /translation="MRLADFDVGPQTPLFLIAGPCVIESEALVMTTAERLKAITAKLA
                     MPLVFKASFDKANRSSLGGFRGLGVEEGLRILERVRADLDVPVLTDVHEDTPLDEVAA
                     VVDVLQTPALLCRQTDFIVAVASQGKPVNLKKGQFLAPWEMTNVVDKARSTGNDALLV
                     CERGVSFGYNNLVVDMRSLAILRATQCPVVFDATHAVQQPGARGASSGGEREHVPVLA
                     RAAVAAGVAGLFMETHPDPEQALCDGPNAWPLASVAVLLEQLQAIDAAVKAQPYLESD
                     FQRVH"
     gene            1607..>2338
                     /gene="eno"
                     /locus_tag="VM692_00210"
     CDS             1607..>2338
                     /gene="eno"
                     /locus_tag="VM692_00210"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780197.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HUQ50607.1"
                     /translation="MSAIKDVMGLEVIDSRGNPTVEAEVVLASGARGRAIVPSGASTG
                     SREAHELRDGDKKRYLGKGVTKAVANVNGEIRKAVAGMDAAGQGELDRRLIELDGTAN
                     KARLGANAILAVSLANAKAHAADRGVALYELLGAGRATYTMPLPMMNIINGGAHADNS
                     VDIQEFMVLPVGAPTFAECLRYGVEVFHALKAVLKAKGMNTAVGDEGGFAPDLGSNEE
                     ALQTIAEAVEKAGYELGRDVYLGLDV"
BASE COUNT          401 a          755 c          795 g          387 t
ORIGIN      
        1 ttcgtacatg tcgctgaccg aagcgctgcg ccacgcgggc attcgcacgc gcacgcgcgt
       61 caacatcctc tacatggagt ccgaggagat tcagacccaa ggcacctcct gcttgaacga
      121 cgtcgacgcg atcctcgtgc ccggcggatt cggtgagcgc ggcatcgaag gcaagatcgc
      181 ggccgtcaag ttcgcgcgcg agaagcaagt gccttatttg ggtatctgct tgggcatgca
      241 ggtcgcaacg atcgaatacg cgcgcaacgt cgcggggctc gcgggcgcgc actcgacgga
      301 gctgaatcgt gagacgccgc atcccgtgat cgcgatgatt accgagtggc aggaccgcga
      361 cggctcgatt caggagcgca acgccgcgtc ggatctcggc ggcacgatgc ggctcggcgc
      421 gcagacgtgc tacgtcaagg acggcacgct ggcgaagcag atttacggcg ccgacgaaat
      481 ctccgagcgc catcgtcatc gctacgagtt caacaaccgc tatctcgagc agctctcgaa
      541 ggccgggctc gtgttctcgg ggttctcgcg cgacgatctc gtcgagctca tcgagttgcc
      601 gccggcagtg cacccgtggt tcttcgcgac ccagttccat ccggagttca actcgaaccc
      661 gcgcgacggc cacccgctgt tcacgagctt catttcggcg gcgcgcgcct gccaaggcct
      721 caagctgccg cgagtcgcga gcgcatgagg ctcgcggact tcgacgtcgg cccgcaaact
      781 ccgctgttcc tgatcgccgg cccttgcgtc atcgagagcg aggcgctcgt catgactaca
      841 gccgagcgcc tcaaggccat cacggccaag ctcgccatgc cgctcgtgtt caaggcctcg
      901 ttcgacaaag ccaatcgcag ctcgctcggc ggctttcgcg ggctcggcgt cgaggagggc
      961 ttgcgcatcc tcgagcgcgt gcgcgccgat ctcgacgtgc ccgtgctgac agacgtccac
     1021 gaggacacgc cgctcgacga ggtcgccgcc gtcgtcgacg tcttgcagac gccggcgctc
     1081 ttgtgccggc aaacggattt catcgtcgcc gtcgcaagtc agggcaagcc cgtcaacttg
     1141 aagaagggtc agttcctcgc gccgtgggag atgacgaacg tcgtcgacaa ggcgcgctcg
     1201 acgggcaacg acgcgctgct cgtctgcgag cgcggcgtct cgttcggtta caacaacctc
     1261 gtcgtcgaca tgcgctccct tgcgatcctg cgcgcgacgc agtgccctgt cgtgttcgac
     1321 gccacgcacg ccgtgcagca gcccggtgcg cgcggcgcgt cgtcgggtgg cgagcgcgag
     1381 cacgtgccgg tgttggcgcg cgcggccgtc gccgccggcg tcgccggttt gttcatggag
     1441 acgcatccgg acccggagca ggcgctctgc gacgggccga atgcgtggcc gctcgcgagc
     1501 gtcgccgtgt tgctcgagca gttgcaggcc atcgacgccg ccgtcaaagc gcagccttat
     1561 ctagaaagcg actttcagcg cgtgcactag tgattcagga gcagtgatga gtgcgatcaa
     1621 ggatgtgatg ggcctcgaag tcatcgattc gcgggggaat ccgacagtgg aggcggaggt
     1681 ggtcttggcg agcggcgcgc gcggccgcgc catcgtgccg tcgggcgctt cgacgggttc
     1741 gcgcgaggcg cacgagctgc gcgacggcga caagaagcgc tatctcggaa aaggcgttac
     1801 caaggccgtc gcgaacgtca acggcgagat ccgcaaagcc gttgcgggca tggacgcggc
     1861 cggacagggc gagctcgatc ggcgcctcat cgagctcgac ggcacggcca acaaagcacg
     1921 cctcggagcg aacgcaatcc tggccgtctc gctcgcgaat gcgaaggcgc acgcggcgga
     1981 ccgcggcgtt gctttgtacg agctgctcgg cgccgggcgg gcgacgtaca cgatgccgtt
     2041 gccgatgatg aacatcatta acggcggcgc gcatgccgac aacagcgtcg acattcagga
     2101 gttcatggtg ctgccggtcg gcgcgccgac gttcgccgaa tgcttgcgtt acggcgtcga
     2161 ggtctttcac gcgctgaagg ccgtgctgaa ggcgaaggga atgaacacgg ccgtgggcga
     2221 cgagggcggg ttcgcgcccg acttgggctc gaacgaggag gccctgcaga cgatcgccga
     2281 agccgtcgag aaggccggct acgagctcgg ccgcgacgtt tatctcgggc tcgacgtt
//
LOCUS       DATLEE010000009         3768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5222806, whole genome shotgun sequence.
ACCESSION   DATLEE010000009 DATLEE010000000
VERSION     DATLEE010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5222806"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VM692_00215"
     CDS             complement(<1..194)
                     /locus_tag="VM692_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HUQ50608.1"
                     /translation="MKAIVLDGFGGPEVLKVGEAAPPVAGPGRVLIRVHATSVNRPDI
                     VQRQGNYPPPPGESPLLGLEA"
     gene            322..1515
                     /locus_tag="VM692_00220"
     CDS             322..1515
                     /locus_tag="VM692_00220"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009838734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HUQ50609.1"
                     /translation="MFETLKPLPPDAILGIMTLFRADPHPGKIDLSVGVYQDEQGRTP
                     VLASVKRAEKTILEKQDSKTYVAIAGNAGFNRGIEEVLYGKDHPALKAGRISTIQTPG
                     GSGGLSIAGHLVARAKPNAKIYVSDPTWPNHQPLLKVSGLTLDTYPYYDYAKHRVDFE
                     PMLARLEKASAGELVLIHGCCHNPCGADLSREQWQALTQLCERRGLVPFIDIAYQGLA
                     EGLDEDAYGVRLMAERLPEVVVVTSCSKNFGLYRERVGAASVVSANAEAAKLSAANAA
                     NVARGIYSMPPDHGAAIVAHIFGDAALRAQWVQELAEIRSRLNGLRKLLVDKLTARKT
                     SMDFSFIARERGMFSFLGITKEQVIRLREEFHVYMVESSRVNIAGINHANVDRVADSI
                     AAVLK"
     gene            complement(1612..2442)
                     /locus_tag="VM692_00225"
     CDS             complement(1612..2442)
                     /locus_tag="VM692_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HUQ50610.1"
                     /translation="MRRLVRQFDLDITGIHGTGPDGRIRVGDVMGLLGGRTDSGNRDA
                     PARAVDPVNDDDRSGADDVALEPPAGSAQRANTVEEAAHAGVPTTTVFDCDLSRVLSH
                     RKKLRRDSVELLTTSYFLTALAAALDSAPEITAGHAPRFGLSLSTVDGAVRSALVDVA
                     TVEWDAALDKRVRAVDAALRATLDADLAAANLLVHHYGESGGLLASPTPIGAGHTASV
                     GIGRVRREIVVRTVDGVESPRVAARCYVSLSFFAEDVPLHHANKCLAEAVRLLEQWPD
                     "
     gene            2662..3024
                     /locus_tag="VM692_00230"
     CDS             2662..3024
                     /locus_tag="VM692_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50611.1"
                     /translation="MKLKHVVILSAFAPGAALAADFSGTWRVDNLFNGAHAIITCTLA
                     QSGNELSGSCKPDIPNIAPSELKGTVDGSSAKWGYDVVFNGNAARVDYEAQLDADGTL
                     KGNVLRNGSPSPITAVKQ"
     gene            3028..>3768
                     /locus_tag="VM692_00235"
     CDS             3028..>3768
                     /locus_tag="VM692_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ50612.1"
                     /translation="MRLPSPYDRLLLPVYVPSLLMAVSQEALTILLPLYMLDIGASPA
                     LAALVVGLRGVGILLFDVPAGMLVARFGDKPVLLGGLALILLGFILLSVTTEPALIGF
                     AAVALGTGHSAWMLGRQSYLADICTTNELGRAIAAMAGLQRGGALIGPFAGGALAGVA
                     GFPVALAVGAASAVVAGATVLAFARAVPPHENPDAGLAGTMQVLRHQRGVLATAGGSA
                     LVLQLMRATRQLLIPLFGEAVGLDVTTIG"
BASE COUNT          590 a         1253 c         1287 g          638 t
ORIGIN      
        1 gcctcgagcc cgagcagcgg cgactcgccc ggcggaggcg ggtagttgcc ctgtcgctgc
       61 acgatgtcgg gccggttcac gctcgtcgcg tgcacgcgaa tcaggacgcg ccccgggccg
      121 gcgacaggcg gtgcagcttc accgactttc agcacctcgg ggccgccgaa accgtcgagc
      181 acgattgctt tcacgccgcc tcttcaatag acgaattcga cgcgccgtgg gcgcgcttgt
      241 tgtgatgccg gagcgatctt catactatac cgccccgccc aaagggcctc cctgccagac
      301 tccccaatca aggactgagc cgtgttcgaa acactgaagc cgctgccgcc cgacgcgatc
      361 ctggggatca tgaccctgtt tcgcgccgat ccgcaccccg gcaagatcga tctgtccgtc
      421 ggtgtctatc aagacgagca gggccgcact cccgtgctcg cgagcgtcaa gcgcgcggag
      481 aaaacgatcc tcgagaagca ggactcgaag acgtatgtcg cgatcgccgg caacgcgggt
      541 ttcaatcgcg gcatcgagga agtgctctac ggcaaggatc atccggcgct gaaggccggc
      601 cgcatctcga cgatccagac gcccggcggc agcggtggct tgagcatcgc gggccacttg
      661 gtcgcacgcg cgaagccgaa cgcgaagatc tacgtcagcg atccgacgtg gccgaaccat
      721 cagccgttgt tgaaggtctc gggccttacg ctcgacacgt acccgtacta cgactacgcc
      781 aagcatcgcg tcgatttcga gccgatgctg gcgcgcctcg agaaggcgag cgccggcgag
      841 ctcgtgctga tccacgggtg ctgtcacaac ccgtgcggcg cggacctctc gcgcgagcag
      901 tggcaagcgc tgacgcagct ttgcgagcgc cgcggcctcg tgccgttcat cgacatcgcc
      961 taccaaggtc tcgccgaagg cctcgacgag gacgcgtacg gcgtgcggct gatggccgag
     1021 cgtttgcccg aagtcgtcgt cgtgacgtcg tgctcgaaga acttcggtct ctaccgcgag
     1081 cgcgtcggcg cggccagcgt cgtctcggcg aacgccgaag cggcgaagct gtcggccgcg
     1141 aacgcggcga acgtcgcgcg cggcatctac tcgatgccgc ccgatcacgg cgccgcgatc
     1201 gtcgctcaca tcttcggcga cgcggcgttg cgcgcgcagt gggtgcagga gctcgccgag
     1261 attcgctcgc ggctgaacgg cctgcgcaag ctgctcgtcg acaagctgac ggcgcgcaag
     1321 acgtcgatgg acttctcgtt catcgcgagg gagcgcggca tgttctcgtt cctcggcatc
     1381 acgaaagagc aggtgattcg cctgcgcgag gaattccacg tttacatggt cgagtcgagc
     1441 cgcgtgaaca tcgcgggcat caaccacgcg aacgtcgacc gcgtcgcgga ctcgatcgcc
     1501 gccgttctca aatagcaatc ggtcggccgt accaaggcaa ggtgccgcca tcacgcaccg
     1561 gcggaaccta atccgtttgt gccgagcctc ttttgctttt ctcgttaagg gttagtcggg
     1621 ccactgctcc agaagtcgca cggcttcggc gaggcacttg ttggcgtggt gcagcgggac
     1681 gtcctcggcg aagaacgaca gcgacacgta gcagcgtgcc gcgacgcgcg gcgattcgac
     1741 gccgtcgacg gtgcggacga cgatctcgcg ccgcactcgg ccgatgccga cgctcgcggt
     1801 gtgccccgcg ccaatgggcg tcggcgacgc gagtaagccg ccgctctcgc cgtagtggtg
     1861 cacgagcagg ttggcggctg cgaggtccgc gtcgagcgtc gcacgcagcg ccgcgtctac
     1921 ggcgcgcacg cgcttgtcga gcgccgcgtc ccactctacg gttgcgacgt cgacgagcgc
     1981 cgagcgcact gcgccgtcga ccgtcgacag cgacaagccg aagcgcggcg cgtggccggc
     2041 cgtgatctcc ggtgccgagt cgagcgcggc cgcgagcgcc gtcaggaaat aactcgtcgt
     2101 caacagctcg acgctgtcgc gccgcaattt cttgcgatgc gatagcacgc ggcttagatc
     2161 gcagtcgaag acggtagtcg tcggtacgcc ggcatgtgcc gcttcttcga cggtgttggc
     2221 ccgctgcgcg ctgccggctg ggggctcgag cgcgacgtcg tctgcgccgg aacggtcgtc
     2281 gtcgttcacg ggatcgacgg cgcgggccgg agcgtcgcga ttgcccgagt ccgtgcgccc
     2341 gccgagcaag cccatgacgt cgccgacgcg gatgcggccg tcgggccccg tgccgtgaat
     2401 tcccgtgatg tcgaggtcga actgccgcac gaggcgtcgc acagccgggc tcagcgagtc
     2461 gtgggccgag tcggcagccg tagccgacgc tgcggactta acgtcggccg gtgccgcgtc
     2521 agtttgaggc gaagcgctat ccggcggcgc accgagcggc gctcgatctt cgttcgtgga
     2581 catgcggcaa ggatcgcgag accgcgaggg gatttcaacc tctgtatgat gtgccgcgca
     2641 gccccgcgtc ggagagcctc gatgaagctg aagcacgtcg tgatcttgag tgcattcgcg
     2701 ccgggcgccg cgctcgcggc ggatttttcc ggcacatggc gcgtcgacaa tctgttcaac
     2761 ggcgcgcacg cgatcatcac gtgcacgctg gcgcaatccg gcaacgagtt gagcggcagc
     2821 tgcaagcccg acattcccaa catcgcgccg tccgagctca aaggcacggt cgacggctcg
     2881 agcgcgaagt ggggttacga cgtcgtgttc aacggcaatg cggcgcgcgt cgactacgag
     2941 gcgcagctcg acgcggacgg cacgctgaag ggcaatgtgc tgcgcaacgg cagcccgtcg
     3001 ccgatcacgg ccgtcaagca ataagcattg aggcttcctt ctccttacga tcgtctgctg
     3061 ctgcccgtct acgtgccgtc gttgctgatg gccgtgagcc aggaagcgct gacgattttg
     3121 ttgccgttgt acatgctcga catcggcgcg agcccggcgc tcgcggcgct cgtcgtcggg
     3181 ttgcgcgggg tcggcatctt gctgttcgac gtgccggccg gaatgctcgt cgcgcgcttc
     3241 ggcgacaagc ccgtgctgct cggcggcttg gcgctgatcc tgctgggctt catcttgctc
     3301 agcgtcacga cggagcctgc tctgatcggc ttcgcggccg tcgcgctcgg cacggggcac
     3361 tcggcctgga tgctcggccg acagtcgtac ctcgcggaca tctgcacgac gaatgagctt
     3421 ggccgcgcga ttgccgcaat ggcggggttg cagcgcggcg gcgcgttgat aggcccgttc
     3481 gccggcggcg cgctcgcggg cgtggccgga ttcccggtcg cgctagccgt cggcgccgcg
     3541 agcgccgtcg tcgccggtgc gaccgtgcta gcgttcgcac gcgccgtgcc gccgcacgag
     3601 aatcccgatg ccggtttggc cggcacgatg caggtgctgc gccatcagcg cggcgtgttg
     3661 gcgacggccg gcggcagcgc gctcgtgcta cagctgatgc gtgccacgcg gcaattgctg
     3721 attccgctgt tcggggaagc cgtcggcctc gacgtcacga cgatcggc
//
LOCUS       DATLEE010000010         4610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1498664, whole genome shotgun sequence.
ACCESSION   DATLEE010000010 DATLEE010000000
VERSION     DATLEE010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498664"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /gene="ligD"
                     /locus_tag="VM692_00240"
     CDS             <1..1258
                     /gene="ligD"
                     /locus_tag="VM692_00240"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HUQ50613.1"
                     /translation="GGKGFGALLLAQYRSGVLTYAGRAGSGFAAHDFKVLEPKLAALP
                     RAEPPAAAELSRGAVWLAEGPVVQVKFKQRTPDGMLRQPVFLRVRDDKKPAECVWLDD
                     AQGAPADDGEPAVAAAEPPERPTVAFTNVSKVFWPADGYTKGDLIAYYEGIAEWLLPY
                     LKDRPVVLTRYPDGIDGKSFFQKDAPVYAPSWLRLETMWSEHAEREIRYFVLDDLESL
                     LYVANMGTIPLHVWSSRVAALERPDWCILDLDPKGAPLKHVVEIARYVHALCEDVGLT
                     NFVKTSGSTGLHVLIPLGARYTYEQSRTLAELLARFTAKSLPEIATIVRSVSDRDGKV
                     YLDYLQNGHGRLLVSPFCARPLPGAPVSMPIEWTHVNARLKLERFTIRSARKWLKAHG
                     DPNAGVLTESTDMLAALEALTARLAE"
     gene            1347..2063
                     /locus_tag="VM692_00245"
     CDS             1347..2063
                     /locus_tag="VM692_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HUQ50614.1"
                     /translation="MRKPVTLIAGQFGSRHFGTKPYLQAAVELTRKEAPIGLYIGAAS
                     GEDDSFGTALCALIATAGAKDVIWPKLAVRKRDAVAARKALERVDFVFVGGGDVEAGM
                     DVLQRAKLVEDLRAAADRGVVFAGMSAGAIMLGERWVRWPRADAGDDEAETYECLGVA
                     PCSLDTHGEGDEWGETRSFAAVRARELGRKAKAYGVTSGGALIVTSGGKMQARGQPVP
                     VFVAEPRGEAKIERTISPEP"
     gene            2060..3298
                     /locus_tag="VM692_00250"
     CDS             2060..3298
                     /locus_tag="VM692_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50615.1"
                     /translation="MSYSRAHWGRVGEETRRMVAEVPLYRDRPEPPADPAAVDQWLAR
                     VPAVGKRELRRGFPKSLLRAHQDLSAAMRDERVTLLATSGTTADRLQVIWEWSWWDPQ
                     EREAMRLNARIAAAMGRNEWREAVLTTPACGGGTCHFGSQTAAERSIDGILFFNESAD
                     PTHWTDGEFERMLREWCEFAPLGVEADPAYLAIMARAALKRGIRLPSPEFITLTYETT
                     TQAMRRDIARAFDAPVFQLYGATEAGVLFMECEHGRLHPNERHSHVDLDALPGTDGLA
                     RVLVTTLGRAWMPLLRYDIGDVVRVAKSRECACGMLSDGPLLERVEGRQSDCTDVDGV
                     TMTPLMLDDAIYAAVGGAESTLEQWQLAGDELLVVDPRSDSAAGVAAVVSALLKRRIR
                     GQRVAAIAPEASGKYRLVKR"
     gene            3298..4470
                     /locus_tag="VM692_00255"
     CDS             3298..4470
                     /locus_tag="VM692_00255"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HUQ50616.1"
                     /translation="MRPEGFPLLERGYDGAPFIYLDSASTTPKPREVIAAVVRYYETL
                     GANVHRGLYPLAEAATEEYERARHRVAALINAQPSEIVFTRNATDSFNLVARALALTQ
                     DDEVVFPASEHHSNYLPWRVAARPVLVDLDDEAVPQWSQLKSRLGKRTRLVTVAHASN
                     VTGVVAPLEEWIATAHAAGVPVLVDASQSIAHLPIDVRALDVDFLGFSSHKMFGPNGV
                     GVLYVRRDRFADLRLGNVGGGMVALLADDRFEPLEAPFRYEAGTPNVEGVIGLGAAVD
                     YLLRVGMPTIAEHSRALATQLIEGLSGLPQATVLGRSAGERIALATVSLPWPAMRQQD
                     IARLLADAHAIFVSGGFHCAHVLHHRLKLDGTLRASAHIYNDSADIDAFIKALHDL"
BASE COUNT          711 a         1535 c         1627 g          737 t
ORIGIN      
        1 aggcggcaaa ggcttcggcg cgctgctgct cgcgcaatat cgcagcggcg ttttgacgta
       61 cgcggggcgt gccggcagcg gcttcgcggc ccacgatttc aaggtgctcg agcccaagct
      121 cgcggcgctg ccgcgcgcgg agccgcccgc ggccgccgag ctgtcgcgcg gcgccgtgtg
      181 gctcgccgag gggcccgtcg tgcaggtcaa gttcaagcag cgcacgcccg acggtatgtt
      241 gcggcagccg gtgtttctgc gcgtgcgcga cgacaagaag ccggccgagt gcgtttggtt
      301 ggacgacgcg caaggcgcgc ccgctgacga cggcgagccg gccgtcgcgg ccgccgagcc
      361 gccggagcgg ccgacggtcg ccttcacgaa tgtctcgaag gtgttttggc ccgcagacgg
      421 ctacacgaag ggcgatctca tcgcctacta cgaaggcatc gccgagtggc tgctgcctta
      481 cctcaaagac cggcccgttg tgctgacgcg ctatcccgac ggcattgacg gtaagtcgtt
      541 ttttcagaag gacgcgcccg tctatgcgcc gagctggctc aggctcgaga cgatgtggag
      601 cgagcacgcc gagcgcgaga tccgctactt cgtgctcgac gatctcgagt cgctgctcta
      661 cgtcgccaac atgggcacga ttccgctgca cgtctggtcg agccgcgtcg cggcgctcga
      721 gcggcccgac tggtgcatcc tcgacctcga cccgaaaggc gcgccgctga agcacgtcgt
      781 cgagatcgcg cgctacgtgc acgcgctctg cgaggacgtc ggcttgacga acttcgtgaa
      841 aacgagcggc tcgacgggtc tgcacgtgct gatcccgctc ggcgcgcgct atacctacga
      901 gcaatcgcgc acgctcgccg agttgctggc gcgcttcacg gccaagtcgc tgccggagat
      961 cgcgaccatc gtccgctcgg tctccgaccg cgacggcaag gtctatctcg actatttgca
     1021 gaacggccat gggcgcttgc tcgtctcgcc gttttgcgcg cggccgttgc ccggagcgcc
     1081 ggtgtcgatg ccgatcgaat ggacgcacgt caacgcgcgc ctgaagctcg agcggttcac
     1141 gatccgctcg gcgcgcaaat ggctcaaggc gcacggcgat cccaatgcgg gcgtgctgac
     1201 ggagtcgacg gacatgctgg ccgcgctcga ggcgctcacg gcccgcctcg ccgagtgagc
     1261 acgagggtgc cgctgttgcg gttcagttga accgaaaccg tagcaccggc acctctgcta
     1321 gagtcgcacc tcgagaagga gctcgtttgc gtaaacccgt gacgttgatc gccggccaat
     1381 tcggcagccg gcatttcggt accaagccct atctgcaagc cgcggtcgag ctgacgcgca
     1441 aggaggcgcc gatcgggctg tacatcggcg ccgccagcgg cgaggacgac tcgttcggta
     1501 cggcgttgtg cgcgctgatc gcgacggccg gcgccaagga cgtgatctgg ccgaagctcg
     1561 ccgtgcggaa gcgcgacgcg gtcgccgcgc gcaaggcgct cgagcgcgtc gatttcgttt
     1621 tcgtcggcgg cggcgacgtc gaggccggca tggatgtctt gcagcgcgcg aaactcgttg
     1681 aggatctgcg cgctgcagcg gatcgtggtg tcgtatttgc cggaatgtcg gcaggcgcga
     1741 tcatgctcgg cgagcgctgg gtgcgctggc cgcgcgccga cgccggcgac gacgaggccg
     1801 agacgtacga atgcctgggt gtggcgccgt gctcgctcga tacgcacggc gagggcgacg
     1861 agtggggcga aacgcgctcg ttcgcggcgg ttcgggcgcg tgagctcggg cgcaaagcca
     1921 aggcctacgg tgtgacgagc ggcggcgcgc tgatcgtgac cagcggtggc aaaatgcagg
     1981 cgcgcggcca gcccgttccg gtgtttgttg ccgagccgcg cggagaagcc aagatcgaaa
     2041 ggacgatttc acccgagcca tgagctattc gcgagcacat tgggggcgcg tcggcgaaga
     2101 gacgcggcgc atggtggccg aggtgccgct gtaccgcgat cggccggagc cgccggccga
     2161 tcccgccgcc gtcgaccagt ggttagcgcg agtcccggcc gtcggcaagc gcgagctgcg
     2221 ccgcggtttt ccgaaatcgc tcctccgcgc ccaccaggat ttgagcgccg cgatgcgcga
     2281 cgagcgtgtc acgctgctcg cgacgtcggg gacgacggcc gatcgcctgc aagtcatctg
     2341 ggaatggtcg tggtgggacc cgcaagagcg cgaggcgatg cggctcaacg cgcggatcgc
     2401 cgcggcgatg gggcgcaacg aatggcgcga agccgtcttg acgacgccgg cttgcggcgg
     2461 cggcacgtgt cacttcggga gccagacggc agccgagcgc agcatcgacg gcatcctgtt
     2521 cttcaacgag agcgcagacc cgacgcattg gaccgacggt gagttcgagc gaatgctgcg
     2581 cgagtggtgc gagttcgcgc cgctcggcgt cgaggccgac cccgcctatc tcgcgatcat
     2641 ggctcgcgcg gcgctgaagc gcggcatccg cttgccgtcg cccgagttca tcacgttgac
     2701 ctacgagaca acgacgcaag cgatgcggcg cgacatcgcg cgcgcgttcg acgcgcccgt
     2761 gtttcagctc tacggggcga ccgaggccgg cgtgctgttc atggagtgcg agcacggccg
     2821 gctgcatccg aacgagcgcc atagtcacgt cgatctcgac gcgctgccgg gcacggacgg
     2881 gttggcgcgc gtgctcgtga cgacgctcgg tcgcgcttgg atgccgctgc tgcgctacga
     2941 catcggcgac gtcgtgcgcg tcgctaaatc gcgcgaatgc gcctgcggca tgctgagcga
     3001 cgggccgttg ctcgagcgcg tcgagggccg gcagagcgac tgcaccgacg tcgacggcgt
     3061 cacgatgacg ccgctgatgc tcgacgacgc gatctatgcc gccgtcggcg gggccgagtc
     3121 gacgctcgag cagtggcagc tggccggcga cgagctgctc gtcgtcgatc cgcgcagcga
     3181 cagcgcggca ggtgtcgccg ccgtcgtcag cgcgctgctg aagcgtagga ttcgcggcca
     3241 acgcgtcgcg gccatcgctc ccgaggcttc gggcaagtac cgactcgtca aacgctgatg
     3301 cggccggaag gctttccgtt gctcgagcgt ggctacgacg gggcgccgtt catctacctc
     3361 gacagcgctt cgacgacgcc gaagccgcgc gaggtcattg cggccgtcgt gcgctactac
     3421 gagacgctcg gcgcgaacgt ccatcgcggg ttgtatccgc tcgccgaagc cgcgaccgag
     3481 gagtacgagc gcgcgcggca ccgcgtcgcg gcgctgatca acgcgcagcc gtccgagatc
     3541 gtgttcacgc gcaacgcgac cgactcgttc aatctcgtcg cacgcgcgct cgcgctgaca
     3601 caagacgacg aggtcgtgtt tcccgcgtcc gagcatcact cgaactactt gccgtggcgc
     3661 gttgccgcgc ggcctgtgct cgtcgatctc gacgatgaag ccgtgccgca gtggtcgcag
     3721 ctgaagtcgc ggctcggcaa acgcacgcgg ctcgtgacgg tcgcgcacgc gtcgaacgtc
     3781 acgggcgtcg ttgcgccgct cgaggagtgg atcgccacgg cgcacgcggc cggcgtgccc
     3841 gtcttggtcg acgcatcgca gtcgattgcg catttgccta tcgacgtccg tgcgctcgac
     3901 gtcgacttcc tgggtttctc gagccacaag atgttcgggc cgaacggcgt cggcgtgctg
     3961 tacgttcggc gcgatcgctt cgcagacctg aggctcggca acgtcggcgg cggcatggtc
     4021 gcgctgctcg ccgacgatcg cttcgagccg ctcgaggcgc cgttccgcta cgaggccgga
     4081 acgccgaacg tcgagggcgt catcggcctc ggcgccgccg tcgactattt gctgcgcgtc
     4141 ggcatgccga cgatcgcgga gcactcgcgc gcactcgcga cgcagctgat cgagggtctc
     4201 tcgggcctac cgcaggcgac cgtgctgggc cgctcggcgg gcgagcgcat cgcgctcgcg
     4261 accgtgtcgc tgccctggcc ggcgatgcgt cagcaggaca tcgcgcggct attggccgac
     4321 gcgcacgcga tcttcgtctc cggcggcttc cactgcgcgc acgtgctgca tcaccgcctg
     4381 aagctcgacg gcacgttgcg tgcgtcggca cacatttata acgattccgc ggacatcgac
     4441 gccttcatca aggcgttgca cgacctgtga cggaggcagc gtcggcgcgc ggcgcctcgg
     4501 ccttcggcgc gagccaaagc cgcagcacga accaagcgaa ggcgagggcg ccgacgctga
     4561 agacgacgtc ccccggaacg cgcagccaca cgagccattc gatcagcggt
//
LOCUS       DATLEE010000011         3134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8948924, whole genome shotgun sequence.
ACCESSION   DATLEE010000011 DATLEE010000000
VERSION     DATLEE010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8948924"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1018
                     /locus_tag="VM692_00260"
     CDS             188..1018
                     /locus_tag="VM692_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HUQ50617.1"
                     /translation="MSEYLTNRRAEAMVLKRRCARILLTGNAFSEPLPTAACFLLEAC
                     VAVKKSELAVLQRRHIMSALALALLPFASHAVVIIDSNVNTAGLYNAGLGDLAALDGA
                     NGFFLAANVSEGDPTLLPVIPQPAVTYGGAFGANWLGGNYSGGTWSAGAVSIPNTWLV
                     NTETAIVYEFTLGALTNLHIDLGVDNGILVWLNGNYVFGAQAPGGSSLGEYAFDLTNV
                     AAGNHRLQILREDHGGATGYNIRADAIAAVVPVPEPASLSLLGVGLLSLGLFRRRRSA
                     "
     gene            1179..2123
                     /locus_tag="VM692_00265"
     CDS             1179..2123
                     /locus_tag="VM692_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HUQ50618.1"
                     /translation="MPHEVVLISGPEIARYGFPHGHPFGTDRHGAFMERLEQSPVAAT
                     LTRRSARQATRAELEYFHTPRYVDRVIELSARGSGLLDAGDTPAFPGVYEAAASVVGG
                     TLEALAAIVAGPVRRAFIPIGGLHHAARDVAAGFCVFNDCGVAIEAARRVYGLRRVAY
                     VDIDAHHGDGVFYAFEQDRDVKFADMHEDGRFLYPGTGSRSETGRGPAVGTKLNIPLP
                     PGADDSVFLDAWQEVERYLEAAEPELILLQCGADSLGGDPITHLKLTAEAHAHAARRL
                     CAIADRFANGRVLAMGGGGYNRANLAAAWTRVVEELAA"
     gene            2120..>3134
                     /gene="glyA"
                     /locus_tag="VM692_00270"
     CDS             2120..>3134
                     /gene="glyA"
                     /locus_tag="VM692_00270"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017376526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HUQ50619.1"
                     /translation="MTFDARSLDEVDPELARALDGELERQETYLELIASENYVSPRVL
                     AAQGSLLTNKYADGYPGARHYAGCELADVAERLAIERAQRLFGAGYANVQPYSGSQAN
                     LAVYLALLSPGDTLLGMRPAHGGHRTHGAADNASGVLYNVVPYDVSAATGTLDYEQIA
                     TLARKHRPKVVVAGFSAYSRVIDWARFRAIADDVGALLVADIAHVAGLIVAGLYPSPV
                     ALADVTTTTTHKTLRGPRGGMILAPRRSPLTERIDAAVFPGTQGGPLMHVIAAKAVAF
                     QEALEPPFAAYQRQVLANARSMAAGLQARRYKIMTGGTDNHMLLVDLRTSAIDAARAE
                     RALH"
BASE COUNT          512 a         1089 c          995 g          538 t
ORIGIN      
        1 ggcaagttag ccaacgcggt ggcaagccac aagatcggac gcggctagct cgagcgagga
       61 ctgaatcggc gcgcgcgcgg cacgggtccc ggccgcgacg cctcacaggt tccccacaaa
      121 cccgcaacct cgagaggccg accgccaggg cgcgcggagt ccttagttat agccctgcag
      181 cccggacgtg tcggaatacc ttacgaatcg acgcgccgag gcgatggtgc tgaagcggcg
      241 atgtgcgcgt atcctcttga ctggtaacgc gttttccgaa ccgcttccga ctgcggcatg
      301 ttttttgctc gaagcctgcg tagcggtaaa aaaatcggag ctcgctgtgc tgcaacgccg
      361 acacatcatg agcgccttgg cgctcgcact tttgcctttc gcgagccacg cagtcgtcat
      421 tatcgactcg aatgtcaata ccgccggcct ttacaacgcc ggcctcggtg atctcgccgc
      481 gctcgacgga gcgaacggat tcttcctcgc tgcgaacgtc agcgaaggcg accccaccct
      541 tcttccggtc atcccgcagc cagcggtcac gtacggcgga gccttcggcg ccaactggct
      601 cggaggcaac tacagcggcg gaacttggag cgctggagcc gtctcgattc caaacacctg
      661 gttggtcaac accgagaccg caatcgttta cgaattcact ctcggcgcgc tgacgaacct
      721 ccatatcgat ctcggtgtcg ataacggaat tctcgtctgg ctaaacggaa actatgtttt
      781 cggcgcgcag gcccccggcg gctcgtcatt aggcgagtat gccttcgatc ttacgaacgt
      841 cgcggcaggt aaccacagat tacagatcct tcgcgaggat cacggtggag ccaccggcta
      901 taacatacgc gcggatgcca tcgctgcggt cgttcccgtc ccggaaccgg ccagtctgtc
      961 gctgctgggc gtggggttac tctcgcttgg gctcttccgc cggcgccgat cggcctagct
     1021 cactgtcagc gttgctgcgc cgccgctagt ccggccgcgg cgcccgttct tctcgtagac
     1081 cccacggctt tctcgtagac ccccgcccac gcgtgcgaga tggagcgcgg acgcctcgca
     1141 cccgcgattg cccgctctcg actacactcg gttgcgagat gccgcacgaa gtcgttttga
     1201 tctcgggccc cgagatcgcc cgctacggat ttccgcacgg tcacccgttc ggcacggatc
     1261 gtcacggcgc cttcatggaa cggctcgagc aatcgccggt ggccgcgacg ctgactcgcc
     1321 gctcggcgcg acaagccacg cgtgccgagc tcgagtattt ccacacgccg cgctacgtcg
     1381 accgcgtcat cgagctatcg gcgcgcggca gcggcctgct cgatgccggc gacacgccgg
     1441 cattcccggg cgtctacgaa gccgcggcta gcgtcgtggg cggcacgctc gaggcgctgg
     1501 cggcgatcgt cgccggaccg gtgcggcgcg cgttcattcc aatcggcggg ttgcaccatg
     1561 cagcgcgcga cgtcgccgcc ggcttttgcg tcttcaacga ttgcggcgtc gcaatcgaag
     1621 ccgcgcgccg tgtgtacggc ctgcgccgcg tcgcctacgt cgacatcgac gcgcatcacg
     1681 gtgacggcgt gttctacgcg ttcgagcaag accgcgacgt caagttcgcg gatatgcacg
     1741 aggacggacg attcctgtat cccggcaccg gcagccggag cgaaacgggg cgcgggccgg
     1801 ccgtcggtac gaagctcaat attccgctgc cgcccggcgc cgacgattcg gtgtttctcg
     1861 acgcgtggca agaggtcgag cgctacctcg aggcggccga gccggagttg atattgctgc
     1921 aatgcggcgc cgattccctc ggcggcgatc cgatcacgca tctgaagctc acggctgaag
     1981 cgcacgcgca cgccgcgcgc aggctctgcg ccatcgcgga tcgcttcgcc aatggccgtg
     2041 tgctcgcgat gggcggcggc ggctacaacc gcgcgaacct cgcggccgcg tggactcgcg
     2101 tcgtggaaga gctcgccgct tgaccttcga cgcgcgcagc ctcgacgaag tcgatcccga
     2161 gctcgcgaga gcgctcgacg gggagctcga gcgccaagag acttacctcg agctcattgc
     2221 ctcggagaac tacgtgagcc cgcgcgtgct cgcggcgcaa ggctcgctgc tgacgaacaa
     2281 gtacgccgac ggttatcccg gcgcgcggca ttacgccggc tgcgagctcg ccgacgtcgc
     2341 cgagcgcctc gcaatcgagc gagcccagcg cctattcggc gccggctatg cgaacgtgca
     2401 gccgtattca ggctcgcaag cgaatttggc cgtatacctc gcgttgctgt ctcccggcga
     2461 cacgctgctt ggcatgcgtc cggcgcacgg tgggcatcgc acgcacgggg cggcggacaa
     2521 cgcctccggc gtgctctaca acgtcgtgcc gtacgacgtt tcggccgcaa cgggcacgct
     2581 cgactacgag cagattgcga cattggcgcg caagcaccgc cccaaagtcg tcgtggccgg
     2641 tttctcggcg tactcgcggg tcatcgattg ggctcgtttt cgtgcgatcg cggacgacgt
     2701 cggcgcgctg ctcgtcgccg acatcgcgca cgtcgccggg ctcatcgtcg ccggcttgta
     2761 tccgagcccc gtcgcgctcg ccgacgtcac gacgaccacg acgcacaaga cgctgcgcgg
     2821 gccgcgcggc ggcatgattc tcgcgccgcg ccgctcgccg ttgaccgagc gcatcgacgc
     2881 ggccgtgttc cccggcacgc aaggcggccc gctgatgcac gtcatcgcgg cgaaggccgt
     2941 cgcgtttcaa gaggcgctcg agccgccgtt cgccgcctat cagcgccaag tcctcgcgaa
     3001 cgcacgctcg atggccgcgg ggcttcaggc gcgccgctac aaaatcatga ccggcggcac
     3061 cgacaaccac atgctgctcg tcgatctgcg caccagcgcg atcgatgccg cacgagccga
     3121 gcgagcgctg cact
//
LOCUS       DATLEE010000012         8296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5226152, whole genome shotgun sequence.
ACCESSION   DATLEE010000012 DATLEE010000000
VERSION     DATLEE010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5226152"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VM692_00275"
     CDS             complement(<1..247)
                     /locus_tag="VM692_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HUQ50620.1"
                     /translation="MQYIDGFIAAVPTANREKYRQHAAEAAEVFKKFGALSVVECWGD
                     DVPEGKLTSFPMAVQRKADETVVFSWITWPSRAVRDDG"
     gene            complement(347..913)
                     /locus_tag="VM692_00280"
     CDS             complement(347..913)
                     /locus_tag="VM692_00280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HUQ50621.1"
                     /translation="MTMILGLSGSLRSGSLNSSLLRAAAEEMPKGVTLEIGSIKGIPL
                     YDGDVEANDGIPDSVAQLKERVVATDGLLLVTPEYNNSLPGVFKNAIDWLTRPPADIA
                     RVWSNRPVGVIGASPGGFGTLLSQTAWLPVLKTLGTAAYFGGRLQVSRAGDVFDANGR
                     MVDPRMREQLKKYLAGFAEFVTASSRSK"
     gene            complement(976..2364)
                     /gene="zwf"
                     /locus_tag="VM692_00285"
     CDS             complement(976..2364)
                     /gene="zwf"
                     /locus_tag="VM692_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HUQ50622.1"
                     /translation="MAEVADALVLFGITGDLAHKKIFPALQELIQHGKLDVPVIGVAR
                     GDMTVDQLRERIRDSLEASDEGVDEGALTRLTELLRYVDGDYRDAATFRALRKVLGAA
                     KHPLHYLALPPSLFATVAQALRESGCADGASVVVEKPFGRDLASALELNDALHRSFDE
                     QAVFRIDHYLGKESVQNLLYFRFANSFLEPIWNRNYVDNVQITMAEDFGVGTRGKFYE
                     EVGAIRDVVQNHLLQVVAQLAMEPPVNAEADALLTEKAQVFKAIRTAHLSDLVRGQYR
                     GYREENGVARNSNVETYAALRLHIDSWRWAGVPFYLRAGKLLDTTATEVHVTLKGPPH
                     RVFGGTDAPNYLRFRLGPDRVAIALGASAKKPGAEMVGRDLELFVCNSGADETGAYER
                     LIGAALHHDRSLFAREDGVLEAWRIVDPLLRVSSAPHEYEPGTPGPSAADALIASSGG
                     WHAVHADTEECP"
     gene            complement(2370..3377)
                     /gene="gnd"
                     /locus_tag="VM692_00290"
     CDS             complement(2370..3377)
                     /gene="gnd"
                     /locus_tag="VM692_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HUQ50623.1"
                     /translation="MQLGLIGLGRMGGNMARRWLEAGHECVGYARSKADLETLSRDGI
                     VPASSLADLVARLKPPRAIWIMIPAGAVEAVIEELEPQLAAGDTLIDGGNSHYVDDVR
                     RSTALDKRGVHYLDVGVSGGIWGREEGYCLMIGGDAATATRLTPLFTALAPSAAGVPP
                     SGSTAAHGYLHCGPSGAGHFVKMVHNGIEYGLMAAYAEGFNLLEQANAGQRAKRIDAE
                     TAPLTVQGTHLAYDFDLAAIAELWRHGSVVRSWLLDLTRDALVRDASLATFRGEVSDS
                     GEGRWTVNAAIEAGVPVPVLATALFARFTSRGHEDFANRLLSAMRREFGGHVERATTA
                     K"
     gene            3507..5654
                     /locus_tag="VM692_00295"
     CDS             3507..5654
                     /locus_tag="VM692_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ50624.1"
                     /translation="MSAISYATAAMLVVTASAALAQSGPPRTARHPFAAERLVAPPTD
                     NWPTNGGNLYNQRYSPLNQIDTANVAQLKGVWRARLRGSGLAPKYSGEGQPVVYDGVA
                     YISTGANDVFALSLDSGEILWQYTADLAEDLPSVCCGWNNRGVAISEDKVFMGRLDGK
                     LVALDRKSGAPVWTIQAERIEENFSITPAPVYYDGLVITGFAGADRGTRGRVKAYDAD
                     DGKLVWTFYVIPGPGEPGHETWPQDNVSWKYGGGAVWQTPAIDPELGLVYFSTGNAGP
                     DYNGAVRAGDNLYTVSIVAVELKTGKYRWHFQQVHHDIWDYDSANPVVLMDLNVAGRA
                     RKAIVEVSKTGWAYILDRVTGEPIVGIDEMPVAQEPLQATAATQPFPRGDAVVPHRIE
                     IAPEGYRLVNEGRIFTPFAGEDPTIVKPGIWGGASWPPSAYDPVKQQLFVCASSVPGG
                     FKGDIDANFKAPTQGGRFGMGQTQQARVTRSGIIAAMDMTTNKVVWRYRWPEQCYSGT
                     LATGGNLLFVGRNDGRLTALDSRTGEQLWEFQTGAGMHAPMSTFVHRGKQYVLAYSAG
                     NALIGSPRGDSVWLFGLEGTLPPVAAGVPVNRLAAAAPAEAAEAAAPAGAATPVTGAP
                     VAAAPAAPEAAVADVGNGRRVYDETCTICHGEDGLGGHGGGAPLDMVPDAGFVLDVVT
                     NGRNNMPSFSTLLKPVDMRDVAAYVAETFGKKQ"
     gene            complement(5662..6327)
                     /locus_tag="VM692_00300"
     CDS             complement(5662..6327)
                     /locus_tag="VM692_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50625.1"
                     /translation="MAYLKKVDHITYACARGTIEKWAWFHIEVEGGVLINRIDDVRPD
                     DPDSSMKIWCIDYGEFGIALIEGIDRKRQSQVTRFVEHHGDHSCQHVAYDCHDLDAFR
                     AHLVRSGCHPRGQTLVRNDGFGTLKQMFAKGYEAGDPTTSGFPEYVQRPRHGDEDLTI
                     TFSQGAGRGFYEQIENAVAAGDVEPFVDFSKMPSDWRVPPPVTKGGDAPTRPHKGESR
                     ARH"
     gene            complement(6344..7264)
                     /locus_tag="VM692_00305"
     CDS             complement(6344..7264)
                     /locus_tag="VM692_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HUQ50626.1"
                     /translation="MGDTPLLLRSMLFVPGDSERKLARAQYVHADALILDLEDSVDAT
                     RKDRARELAAEFIGGRTSIRARTIWVRTNPAADPRFADDVAAAVHARADGIVLPKPRS
                     AADVRDLANRLDELERNADRAVGTTKILPIATETPSSVLALGGYADCGPRLAGLTWGA
                     EDLSEAVGATTAVDSRGGWLPPYELARSLTLFAAAAAGVMAIDTVHTDFTDLDGLRGS
                     AAAAQRDGFAGKLAIHPEQIEILNRTFQPAADAVAAARAVAAAFAAAPGAGVVAHGGR
                     MLDRPHLLRAQRVLAQAAAVDAPTDEDNLH"
     gene            complement(7267..>8296)
                     /locus_tag="VM692_00310"
     CDS             complement(7267..>8296)
                     /locus_tag="VM692_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HUQ50627.1"
                     /translation="TAGGAYVPAMSEETIIVKEQGTIFLAGPPLVKAAIGEEVDEETL
                     GGGDLHTRISGVADHLANDDDHALAMARDIVARTRAPGATLVEARSDCDPKYPPEELY
                     GIVPESFRYQYDVREVLARLVDRSELDEFKARYGTTLVCAFASIWNQPVGIVASNGVL
                     FSESSLKGTHFIELCNRRGVPLLFLQNISGFMVGKDAERRGIAKDGAKLVTAVACARV
                     PKITVIIGASYGAGNYGMCGRAYSPRLLWTWPNARIAVMGGEQAGMVLGSLSKRSGND
                     AAEAEAVRNEIKEQYARQSTAWYASARLWDDGVIDPVDTRRILGLSLATALNAPLPPE
                     TPYGVLRM"
BASE COUNT         1304 a         2807 c         2832 g         1353 t
ORIGIN      
        1 atccgtcgtc gcgcactgca cgcgatggcc aggtgatcca cgagaaaacg accgtctcgt
       61 cggctttgcg ctgcacggcc atcggaaacg aagtcagctt gccttcggga acgtcgtcgc
      121 cccagcattc gacgacgctg agcgcgccga acttcttgaa cacttcggcc gcttcggcgg
      181 cgtgttggcg atatttctcg cggttggcgg tcggaactgc ggcgatgaag ccatcgatgt
      241 attgcatgtc ggtcctcctt gacgaatgtt gtcgagccgc gcgctcgcag tctgcggcac
      301 gcgtacaggt tgggagtcgc gcagcgcgcg ctcggttccc gagcggctac ttcgagcggc
      361 tactcgccgt cacgaactcg gcgaaaccgg ccaagtattt cttcaattgc tcgcgcatgc
      421 gcgggtcgac catcctgccg ttggcatcga agacatcgcc ggcacgcgac acttgcaacc
      481 gcccgccgaa gtaggccgcc gtgccgagcg tcttcaagac gggcagccat gccgtttgcg
      541 agagcaacgt gccgaagccg cccggcgatg cgccgatgac accgacgggg cggttgctcc
      601 agacgcgcgc tatatcggcg ggcggccgag taagccagtc gatcgcgttc ttgaacacgc
      661 cgggcagcga gttgttgtac tcgggcgtca cgagcagcaa gccgtccgtc gcgacgacgc
      721 gctccttgag ctgcgcgacg ctgtccggta tgccgtcgtt ggcttcgacg tcgccgtcgt
      781 acaacggaat gcccttgatg ctgccgatct cgagcgtgac gcccttcggc atttcttccg
      841 cggccgcgcg cagcagcgag ctattcagag agccggagcg caagctgccg gagagcccga
      901 gaatcatcgt catgacactc acctcgcgag tcgttcgcca tcaagcaacg agtctacgcc
      961 gcgcgagggc gcgggttacg gacattcctc ggtgtcggcg tgcacggcat gccaaccgcc
     1021 ggagctcgcg atcagcgcgt cggcggcgct cggccccggc gtgccgggct cgtactcgtg
     1081 cggcgcgctg ctgacgcgca gcagcggatc gacgatgcgc cacgcctcga gcacgccgtc
     1141 ctcgcgcgcg aacagcgaac ggtcgtggtg cagcgcggcg ccgatgagcc gctcgtaggc
     1201 gccggtctcg tcggcgccgg agttgcagac gaacagctcg aggtcgcggc cgaccatttc
     1261 ggcgccgggt ttcttcgccg acgcgccgag cgcaatcgca acgcgatccg ggccgagccg
     1321 aaaacgcagg tagttcggcg catcggtgcc gccgaagacg cggtgcggcg gccccttcag
     1381 cgtcacgtgc acctcggtcg ccgtcgtgtc gagcagcttg ccggctcgta gataaaacgg
     1441 cacgccggcc cagcgccacg aatcgatgtg caagcgcagc gccgcgtacg tctcgacgtt
     1501 cgaattgcgt gccaccccgt tttcttcacg gtagccgcga tactggccgc gcacgaggtc
     1561 gctcaagtgc gccgtgcgaa tcgccttgaa gacctgcgct ttctcggtca gcagcgcgtc
     1621 ggcctcggcg ttgacgggcg gctccatcgc gagctgcgcg acgacttgca gcagatggtt
     1681 ctgaacgacg tcgcggatgg cgccgacttc ctcgtagaac ttgccgcgcg tccccacgcc
     1741 gaaatcttcg gccatcgtga tctgcacgtt gtcgacgtag ttgcgattcc aaatcggctc
     1801 gaggaacgag tttgcgaacc ggaagtagag caggttctgc accgactcct tgccgaggta
     1861 gtgatcgatg cgaaacaccg cttgctcgtc gaagctgcga tgcagggcgt cgttgagctc
     1921 gagcgccgag gccaagtcgc ggccaaacgg cttctcgacg acgacgctgg cgccgtcggc
     1981 gcagccggac tcgcgcagcg cttgtgcgac ggtcgcgaac aagctcggcg gcaaggcgag
     2041 gtagtgcagc ggatgtttgg ccgcgccgag cactttgcgc agcgcgcgaa acgtggcggc
     2101 gtcgcgataa tcgccgtcga cgtagcgcag gagctccgtg agccgcgtga gcgcgccttc
     2161 gtcgacgcct tcatcgctgg cctcgaggct gtcgcgaatg cgctcgcgca gctggtcgac
     2221 ggtcatgtcg ccgcgcgcaa cgccgattac gggcacgtcg agcttgccgt gttgaatcag
     2281 ctcctgcagc gccggaaaga ttttcttgtg cgcgaggtcg cccgtgatgc cgaacagaac
     2341 gagcgcgtcg gcgacttcag ccatgacggc tacttcgccg tggtcgcgcg ttcgacgtgg
     2401 ccgccgaact cgcgccgcat cgccgacagc agccggttcg cgaaatcctc gtggccgcgc
     2461 gacgtgaagc gcgcgaacaa cgccgtcgca aggacgggca ctgggacgcc ggcttcgatg
     2521 gccgcgttga cggtccagcg gccttcgccg gagtccgaga cttcgccgcg gaaggtcgcc
     2581 aggctcgcgt cgcgcaccag cgcgtcgcgc gtcagatcga gcagccacga gcgaacgacg
     2641 ctgccgtggc gccagagctc ggcgatcgcg gcgaggtcga agtcgtaggc gaggtgagtg
     2701 ccctgaaccg tcagcggcgc ggtttcggcg tcgatgcgct tggcgcgttg gcccgcgttg
     2761 gcttgctcga gcaaattgaa gccctcggcg tacgcggcca tgagcccgta ctcgatgccg
     2821 ttgtgcacca tcttcacgaa gtggccggcg ccgctcgggc cgcagtgcag gtagccatgg
     2881 gccgcggtgc tcccgctcgg cggcacgccg gccgcgctcg gcgccagcgc cgtgaacaac
     2941 ggcgtcaagc gcgtcgccgt cgccgcgtcg ccgccgatca tgaggcagta gccttcctcg
     3001 cggccccaga tgccgccgct gacgccgacg tcgagatagt gcacgccgcg cttgtcgagc
     3061 gccgtcgagc gccgcacgtc gtcgacgtaa tgcgagttgc cgccgtcgat cagcgtgtcg
     3121 ccagccgcga gctgcggctc gagctcctcg atgaccgctt cgacggcccc cgccgggatc
     3181 atgatccaga ttgcgcgcgg cggcttcagc cgtgcgacca agtccgcgag cgagctcgcc
     3241 gggacgatgc cgtcgcgact caacgtctcg aggtctgcct tgctgcgtgc gtagcccacg
     3301 cactcgtggc cggcctcgag ccagcggcgc gccatgttgc cgcccatcct gccgaggcct
     3361 atgagaccga gttgcacgcg accgctccct gcttggtaaa tgctcgctgg tgcttgggac
     3421 cactatgcca cgggccggcg ccagaagttg cgtaggcttc tgcgcgtcag ctgtggtaga
     3481 tagagaacga cgactcgagg gggaatatga gcgcaatctc gtatgcgacg gcggcgatgc
     3541 tcgttgtcac ggcttcagcg gcgctcgcgc agtcggggcc gccgcgcacg gcgcggcatc
     3601 cgttcgccgc cgagcgtctg gtcgcaccgc cgaccgacaa ctggcccacg aacggcggca
     3661 atctctacaa ccagcgctac tcgccgctga atcaaatcga cactgcaaac gtcgcccagc
     3721 tcaaaggcgt ctggcgcgcg cggttgcgcg gctcggggct agcgccgaag tactcgggcg
     3781 aagggcagcc cgtcgtctac gacggcgtcg cgtacatcag caccggcgcc aacgacgtgt
     3841 tcgcgctgtc gctcgatagc ggcgagatcc tttggcaata cacggcggat ctcgcggagg
     3901 atctgccgtc cgtctgctgc ggttggaaca accgcggcgt cgccatcagc gaagacaaag
     3961 tgttcatggg ccggctcgac ggcaagctcg tcgcgctcga tcgcaaatcc ggcgcgcccg
     4021 tctggacgat tcaggccgag cgcatcgagg agaacttctc gatcactccg gcgccggttt
     4081 actacgacgg cttggtcatc acgggcttcg cgggcgcgga tcgcggcacg cgcggccgcg
     4141 tcaaagccta cgacgccgac gacggcaagc tcgtctggac gttctacgtg atccccggcc
     4201 ccggcgagcc ggggcatgag acgtggccgc aagacaacgt ctcgtggaag tacggcggcg
     4261 gcgccgtgtg gcagacgccg gccatcgatc ccgagctcgg gctcgtctat ttctcgacgg
     4321 gcaacgcggg ccccgactac aacggcgccg tacgcgccgg cgacaacctc tacacggtgt
     4381 cgatcgtcgc cgtcgagctg aagaccggca aataccgctg gcacttccag caggtgcacc
     4441 acgacatctg ggattacgac tcggcgaatc ccgtcgtgct gatggacttg aacgttgcag
     4501 ggcgcgcgcg caaagcgatc gtcgaggtaa gcaaaacggg ttgggcttac atcctcgatc
     4561 gcgtcaccgg cgagccgatc gtcggcatcg acgagatgcc cgtcgcgcaa gagccgctgc
     4621 aagcgaccgc cgcgacgcag ccgttcccgc gcggcgacgc cgtcgtgccg catcgtatcg
     4681 agatcgcgcc cgagggttac cgcctcgtca acgaaggccg catcttcacg ccgttcgcgg
     4741 gcgaggaccc aacgattgtg aagcccggca tctggggcgg cgcaagctgg ccgccgagcg
     4801 cgtacgaccc cgtgaagcag cagctgttcg tctgcgcgtc gtcggtgccg ggcggcttca
     4861 agggcgacat cgacgccaac ttcaaggcgc ccacgcaagg cggccgcttc ggcatgggtc
     4921 agacgcagca ggcgcgcgtc acgcgcagcg gcatcatcgc cgcgatggac atgacgacga
     4981 acaaagtcgt ctggcgttat cgctggcccg agcagtgcta cagcggcacg ctcgcgacgg
     5041 gcggcaatct gctgttcgtc ggccgcaacg acggccggct gacggcgctc gactcgcgca
     5101 cgggcgagca gctctgggaa tttcaaacgg gcgccggcat gcacgcgccg atgagcacct
     5161 tcgtgcatcg cggtaagcaa tacgtgctcg cgtactcggc cggcaatgcg ctgatcggct
     5221 cgccgcgcgg cgacagcgtc tggctattcg ggctcgaagg cacgctgccg cctgtcgcgg
     5281 ccggcgtgcc cgtgaaccgg ctcgcggcgg cggctccggc cgaggcggcg gaagctgcgg
     5341 cgccggccgg ggcggcaact ccggtgacgg gggctccggt cgcggctgcg cccgccgcgc
     5401 cggaggcggc ggtcgccgac gtcggcaacg gccggcgcgt ctacgacgag acgtgcacga
     5461 tctgccacgg cgaggacggc ttgggcggtc acggcggcgg cgcgccgctc gacatggtgc
     5521 cggatgccgg cttcgtgctc gacgtcgtga cgaacggccg caacaacatg ccgtcgttct
     5581 cgacgctgct gaagcccgtc gacatgcgcg acgtggcggc ctacgtcgcg gagacgttcg
     5641 gcaagaagca gtgaacggcg gctagtgccg cgcgcgcgat tcgcccttgt gcggtcgcgt
     5701 cggtgcgtcg ccgcctttgg tcaccggcgg cggcacgcgc caatcgctcg gcatcttcga
     5761 gaaatcgacg aacggctcga cgtcgccggc ggccacggcg ttctcgatct gctcgtagaa
     5821 gccgcgcccg gcgccttgcg agaacgtgat cgtcaggtct tcgtcgccgt gccgcggccg
     5881 ctgcacgtat tcggggaagc cgctggtcgt cggatcgccg gcctcgtaac ccttcgcaaa
     5941 catttgcttc aaggtcccga agccgtcgtt gcgcacgagc gtttgaccgc gcggatgaca
     6001 gccgctgcgc acgagatgtg cgcggaaggc gtcgaggtcg tggcagtcgt aggcaacgtg
     6061 ttggcacgaa tgatcgccgt gatgctcgac gaagcgcgtg acctgcgatt ggcgcttgcg
     6121 gtcgatgcct tcgatcagcg cgatgccgaa ctcgccgtaa tcgatgcacc agatcttcat
     6181 gctgctgtcg gggtcgtcgg ggcgcacgtc gtcgatgcga ttgatgagga cgccgccttc
     6241 gacctcgata tggaaccagg cccacttctc gatcgtgccg cgcgcgcacg cgtaggtgat
     6301 gtggtcgact tttttcaaat acgccatggc tccctcccgt tcgctagtga aggttgtcct
     6361 cgtcggtcgg cgcatcgacg gccgcggcct gcgcgagcac gcgctgtgcg cgcagcaagt
     6421 gcggccgatc gagcatgcgc ccgccgtgcg cgacgactcc ggcacccggc gccgcggcga
     6481 acgcggcggc tacggcccgg gccgcggcga cggcgtcggc cgccggctgg aacgtgcgat
     6541 tcagaatctc gatctgctcg gggtggatcg cgagcttgcc cgcgaagccg tcgcgctgcg
     6601 ctgcggcggc cgagccccgc aagccgtcga gatccgtgaa atccgtgtgg accgtatcga
     6661 tcgccatgac gccggccgcc gcggccgcga acagcgtcag cgaccgcgcg agctcgtacg
     6721 ggggcagcca gccgccgcgc gaatcgaccg ccgtcgtcgc accgacggct tcgctcaagt
     6781 cctcggcgcc ccacgtcaag cccgcgagcc gcggcccgca gtccgcgtag ccgccgagcg
     6841 cgagcacgct gctcggtgtt tcggtcgcga tcggcaggat cttcgtcgtg ccgacggcgc
     6901 gatcggcatt gcgttcgagc tcgtcgagcc ggttggccag gtcgcgcacg tcggcggccg
     6961 agcgcggttt cggcagcacg ataccgtcgg cacgtgcatg cacggccgcc gcgacgtcgt
     7021 cggcgaagcg cgggtccgcg gccggattgg ttcgaaccca aatcgtccgc gcgcgaatcg
     7081 aggtgcgccc gccgatgaac tcggctgcga gctcgcgcgc gcgatccttg cgcgtcgcgt
     7141 cgacggaatc ctcgagatcg aggatcaacg catcggcgtg gacgtattgc gcgcgcgcca
     7201 gcttgcgctc gctgtcgccg ggcacgaaca gcatcgagcg cagcagtagc ggcgtgtcgc
     7261 ccatggctac atccgcagca cgccgtacgg cgtctcgggc ggcagcggcg cattcagcgc
     7321 cgtcgcgagg ctcaagccga ggatgcggcg cgtgtcgacg gggtcgatga cgccatcgtc
     7381 ccagaggcgc gcgctcgcgt accacgcggt cgactgccgc gcgtactgct ccttgatctc
     7441 gttgcgcacg gcttccgctt cagcagcgtc gttgccggag cgcttgctca agctcccgag
     7501 caccatgccg gcttgctcgc cgcccatgac ggcaatccgt gcgttcggcc acgtccagag
     7561 aaggcgcgga ctgtaggctc gcccgcacat gccgtaattg cccgcgccat aagacgcgcc
     7621 gatgatcacc gtgatcttcg gcacgcgggc gcaagcgaca gccgtcacta gcttggcgcc
     7681 gtccttggca atgccgcggc gctccgcgtc cttgccgacc atgaagcccg agatgttctg
     7741 caggaacagc agcggcacgc cgcgccggtt gcagagctcg atgaagtgcg tgcctttcag
     7801 cgacgactcc gagaacagaa cgccgttgct ggcgacgatg ccgacgggct ggttccagat
     7861 cgaggcgaat gcgcagacga gcgtcgtgcc gtagcgcgct ttgaactcgt cgagctccga
     7921 gcggtccacg agccgcgcga gcacctcgcg cacgtcgtac tggtagcgaa agctctcggg
     7981 cacgatgccg tacagctctt cgggcggata cttggggtca cagtcggagc gcgcttcgac
     8041 gagggttgca ccgggagcgc gcgtgcgcgc gacgatgtcg cgcgccatcg ccagcgcgtg
     8101 gtcgtcgtcg ttcgcgagat ggtcggcgac acccgagatc cgcgtgtgca gatcgccgcc
     8161 gccgagcgtc tcctcgtcga cttcctcgcc gatcgcggct ttgacgagcg gcggaccggc
     8221 cagaaagatc gtgccttgct ccttgacgat gatcgtctct tcgctcatcg cgggcacgta
     8281 cgcgccgccg gccgtg
//
LOCUS       DATLEE010000013         4437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4483121, whole genome shotgun sequence.
ACCESSION   DATLEE010000013 DATLEE010000000
VERSION     DATLEE010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4483121"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VM692_00315"
     CDS             <1..691
                     /locus_tag="VM692_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50628.1"
                     /translation="DEALPMPRNAGAVLPSSILARLPRLDTIKRGTDCTLGNLQAAVE
                     KETAQAVALQDAHYGNPGDSGLKRTAAEGAILARLEDTDLESCGIAYTNDAAYAAESN
                     AASNAFREASGRISEEQQAAWNACPGIPGGKDAACERRVEADAARKLHDAQRRYLATM
                     SQPFAARIATLRTCTVKREALVADAKAADVRGANVKLVLRPLVLAWQQAAFLPAEWSS
                     ICESAQQSLLE"
     gene            702..1640
                     /locus_tag="VM692_00320"
     CDS             702..1640
                     /locus_tag="VM692_00320"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HUQ50629.1"
                     /translation="MSTSTRGACLCGAVTFAIAPPYRWFAHCHCSLCRKHHGSLFSTG
                     LGVARANLEWLGKSPKGGDAIVHYRATAAFERPFCRHCGSTVPGASHDAGFWHVPAGL
                     LQGDPGARPRSHIFVASKSPLFEIADALPRFAAYPPGIDLPTIERRREPAPPGTIVGS
                     CLCNAVAFGVEAMPRRVVNCYCSLCRRRSGAGFTSTLVTTASEFRWLRGQARVTHFKL
                     PPPRQYGADFCADCGSPVPSFAATTVLLPAGAMDDPWKSSKGDDPWKSPGDTDLPALP
                     AVHLYVGSKAPWVTIADEWPQFDALPPPGQLKDYFP"
     gene            complement(1621..2424)
                     /locus_tag="VM692_00325"
     CDS             complement(1621..2424)
                     /locus_tag="VM692_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50630.1"
                     /translation="MNALRIGLAAAVLAVLAAPLAAQQAERPAPRTPAGKPDMTGIWV
                     ATGALLLFEGEEAFAAARAADAAAGRPPGNSNEALPYKPEAEAQRQYFLSRRGIDDPM
                     AQCLLTGVPRINFRPLPFEIVQLPDRMIMLYEIHHAFRIIPTDGRAPPEDAEPSYLGE
                     SVAHWDGDTLVVDVTSFNTGTWLTGVGTIHSEDLKVTERYTRDSFETIRYEATLDDPA
                     VFTKPWTIRETFRLRPDERIREYECIESNEDIVRFQKLLQDGELTESNP"
     gene            complement(2424..2825)
                     /locus_tag="VM692_00330"
     CDS             complement(2424..2825)
                     /locus_tag="VM692_00330"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ50631.1"
                     /translation="MRRFLVLLLLGSGLCAARFAAAHHSFAAVFDGDKSIKMTGEVVD
                     VEWTNPHFHFSLDVKDAGGAVTRWRFEGYPPNMLVRQGWRRDETLMPGATVTVEGWAS
                     RDDATLGAVRWVTFTDGRKLAGGPPAGTGGR"
     gene            complement(2933..>4437)
                     /locus_tag="VM692_00335"
     CDS             complement(2933..>4437)
                     /locus_tag="VM692_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HUQ50632.1"
                     /translation="GQDDWFWIDIPVGYLYTIGNPRTDWCYVTEPEPGLNGRAIGYAR
                     GKVLGGCSSINAMIYMRGQKQDYDHWAALGLRGWSWDDVLPIFKRSEDYEHGADEWHG
                     AGGELRVEERRVSWEILDAWRDAAEECGIPKIAEFNRGDNFGNAYFQMNQRRGVRWSA
                     TKAFLRPVLGRQNLTVRTHALVERVALETRDGVPRATGVVVRSAGEGVQTLRARREVL
                     LAAGSIGSPQLLQLSGLGPAKLLREHGVDVVADLPGVGANLQDHLQVRSIYKVRNTRT
                     LNRRAGNLFGKAAMALEYALKRTGPLTMPPSQLGAFAKSDGSQASANIEWHVQPLSLD
                     KFGSPLHAFDAITPSVCNLQPTSRGHVRIKSADPAAHPKITLNYLATEADRRVAVAGL
                     RLTRRIMAAKALARFAPEEWLPDPKLTTDADLQRAAGELGTTIFHPVGTCQMGIDSLA
                     VVDDRLRVRGVAGLRVVDASVMPRITSGNTNAPTVMIAEQAASFIARSAD"
BASE COUNT          726 a         1516 c         1463 g          732 t
ORIGIN      
        1 cgacgaagcg ctaccgatgc cccgcaacgc cggcgcagtg ttgccgtcgt cgattctcgc
       61 gcggctgcca cgtctcgaca cgatcaagcg cggcacggat tgcacgctcg gcaacttgca
      121 ggccgccgtc gagaaggaga ccgcgcaggc cgtggccttg caggacgccc actacggcaa
      181 ccccggcgac agcggcctga agcgcacggc ggccgaaggc gcgatcctcg ctcgcctcga
      241 ggacacggat ctcgagagct gcggaatcgc gtatacgaac gatgcagcgt acgcggccga
      301 gtccaacgcc gcttcgaacg cgttccgcga ggcgtcgggc cgtatctccg aggagcagca
      361 agcagcctgg aacgcgtgcc ccggaattcc gggcggcaaa gacgcagcat gtgagcggcg
      421 tgtcgaggcc gacgcggcgc gcaagttgca cgatgcgcag cgccgttacc ttgcgacgat
      481 gtcgcagccg ttcgccgcgc gcatcgcgac gctgcgcacc tgcaccgtga aacgcgaagc
      541 gctcgtagcg gacgccaaag ccgccgacgt tcgcggcgcc aacgtcaagc tcgtcttgcg
      601 tccgctcgtc ttggcttggc agcaagccgc gttcttgcct gcagagtgga gcagcatctg
      661 cgagtcggcg cagcagagct tgctcgagtg acgcgcagcg cgtgagcacg agcacgcgcg
      721 gcgcgtgcct ttgcggcgcg gtgacgttcg cgatcgcgcc gccttaccgc tggttcgcac
      781 attgccactg ctcgctgtgc cgcaagcatc acggcagctt gttcagcacg gggctcggcg
      841 tcgcgcgcgc caacctcgaa tggctcggga agtccccgaa ggggggcgac gccatcgtcc
      901 actatcgcgc gacggcagcg ttcgagcggc cgttctgccg tcactgcggc tcgacggtac
      961 cgggcgcttc gcacgacgct ggcttctggc acgtgcctgc gggcctgttg caaggcgacc
     1021 ccggcgcgcg gccgcgcagt cacatcttcg tcgcgtccaa gtcgccgcta ttcgaaatcg
     1081 ccgacgcgtt gccgcgtttc gcggcgtacc cgcccggcat tgatttaccg acgatcgaac
     1141 ggcgcagaga gccggcgccg cccggcacga tcgtcggcag ctgcctttgt aatgccgttg
     1201 ccttcggcgt ggaggcaatg ccgcgccgcg tcgtgaattg ctattgctcg ctgtgccggc
     1261 gccgaagcgg tgccggattc acgagcactc tggtgacgac ggcgagcgaa ttccgttggc
     1321 ttcgcggtca agcgcgcgtt acgcacttca agttgccgcc gccgcgccag tacggcgccg
     1381 acttctgcgc ggactgcggc agccccgtgc cgagcttcgc cgcgacgacc gtcctgctgc
     1441 cggccggtgc aatggacgac ccttggaagt cctcgaaggg ggacgaccct tggaagtccc
     1501 cgggggatac ggacctgccg gcactgccgg cagtgcatct ctacgtggga tcgaaggcgc
     1561 cgtgggtgac gatcgcggac gagtggccgc agttcgacgc gctaccgccg ccggggcagc
     1621 tcaaggatta ctttccgtga gctcgccgtc ctgcaacaac ttctgaaagc gcacgatgtc
     1681 ctcgttactc tcgatgcact cgtactcgcg aatgcgctcg tcggggcgca gccgaaacgt
     1741 ttcgcggatc gtccacggct tcgtgaagac ggcgggatcg tcgagcgtcg cctcgtagcg
     1801 aatcgtctcg aacgagtccc gcgtgtagcg ctccgtgacc tttagatctt ccgagtgaat
     1861 ggtgccgacg cccgtcaacc acgtccccgt gttgaacgac gtgacgtcga cgacgagcgt
     1921 atcgccgtcc cagtgtgcga ccgactcgcc gagatacgac ggctccgcgt cctcgggcgg
     1981 cgcgcggccg tccgtaggga tgatgcgaaa cgcgtggtgg atctcgtaga gcatgatcat
     2041 gcgatccggc agctgcacga tctcgaacgg caacggacgg aagttgatac gcggtacgcc
     2101 ggtcaacagg cactgcgcca tcgggtcgtc gatgccgcgg cgcgagagga agtattgccg
     2161 ctgcgcttcg gcctcgggct tgtacggcaa cgcttcgttc gaattgccgg gcgggcggcc
     2221 tgctgccgca tcggcggcgc gtgccgcggc aaacgcctct tcgccttcga acagcaacaa
     2281 ggcgccggtc gcgacccaga tgccggtcat gtccggctta ccggccggcg tccgcggcgc
     2341 cggccgctcg gcctgctgcg cggcgagcgg agcggccaat acggcgagca cggcggcagc
     2401 gagcccgatg cgcagcgcat tcatcagcga cctcccgtgc cggccggcgg gccgccggcg
     2461 agcttgcggc cgtcggtgaa agtgacccag cgcacggcgc cgagggtcgc atcgtcgcgc
     2521 gacgcccagc cctccacggt gaccgtcgcg ccgggcatca gcgtctcgtc gcgccgccag
     2581 ccttgccgca cgagcatgtt cggcggatag ccttcgaagc gccaacgcgt gacggcgccg
     2641 ccagcgtctt tgacgtcgag cgagaagtgg aaatgcggat tcgtccattc gacgtcgacg
     2701 acctcgccgg tcatcttgat cgacttgtcg ccgtcgaata cggccgcgaa cgagtggtgg
     2761 gcggcagcga agcgtgctgc gcaaagaccg ctgcccagta gcagcaagac gagaaatcga
     2821 cgcatgggga ccgctcctta attgatcgag cgatggctga ttgttcgatt atccgcggcc
     2881 ccgatctttc ccctcaagga atactggcca taacccatgg tcatacgcct tctcagtccg
     2941 cgctgcgcgc gatgaagctg gcagcttgtt cggcgatcat gaccgtcggc gcgttggtgt
     3001 tacccgaagt gatccgcggc atcacggaag cgtcgacgac gcgcaacccc gcgacgccgc
     3061 gcacgcgcaa gcgatcgtcg acgacggcca gcgagtcgat gcccatttgg cacgtgccga
     3121 cgggatggaa aatcgtcgtg ccgagctcac cggccgcgcg ctgcagatcg gcatcggtcg
     3181 tcagcttcgg gtcgggcagc cattcctcgg gcgcgaaccg ggccagcgcc ttggccgcca
     3241 tgatgcggcg cgtcagcctg aggcccgcga cggcgacccg ccggtccgct tcggtcgcaa
     3301 ggtagttcag cgtgatcttg ggatgcgcgg ccggatcggc gctcttgatg cgtacgtggc
     3361 cgcggctcgt cggctgaaga ttgcaaacgg acggcgtgat cgcgtcgaac gcgtgcagcg
     3421 gcgaaccgaa cttgtcgagc gacaacggct gaacgtgcca ctcgatgttg gccgacgctt
     3481 ggcttccgtc actcttcgcg aaagcgccga gctgcgacgg cggcatcgtc aacgggcccg
     3541 tgcgcttgag cgcgtattcg agcgccatcg cggccttgcc gaacaagttg cccgcccgtc
     3601 gattcaacgt ccgcgtgttt cgcaccttat aaatggagcg aacctgcaaa tggtcctgca
     3661 gattcgcgcc gacgccgggc agatccgcga ccacgtcgac gccgtgctcg cgcaacagct
     3721 tcgcggggcc gagacccgat aactgcaaca attgcggcga cccgatcgag cccgccgcga
     3781 gcagcacctc gcgccgcgcg cgcaacgttt gcacgccttc gcctgccgag cgcacgacga
     3841 caccggttgc gcgcggcacc ccgtcgcgcg tctcgagcgc cacgcgctcg acgagcgcat
     3901 gagtccgcac ggtcaagttt tgccggccga gaacgggccg caagaacgct ttcgtcgcgc
     3961 tccatcgcac tccgcgccgc tggttcatct gaaagtacgc gttgccgaaa ttgtcgccgc
     4021 gattgaattc cgcgatcttc gggatcccgc actcctccgc ggcgtcgcgc cacgcgtcga
     4081 gaatctccca gctgacgcgc cgctcctcga cgcggagctc gccgcccgcg ccgtgccact
     4141 cgtcggcgcc gtgctcgtag tcctcggagc gcttgaaaat cggcagcacg tcgtcccacg
     4201 accagccgcg caggccgagc gccgcccaat ggtcgtagtc ctgcttttgc ccgcgcatgt
     4261 agatcatggc gttgatcgac gagcagccgc cgagcacttt gccgcgcgca tagccgattg
     4321 cgcggccgtt caaacccggc tcgggctcgg tgacgtagca ccaatcggtg cgcgggttac
     4381 cgatcgtgta gagatagccg acgggaatgt cgatccagaa ccaatcatcc tgcccgc
//
LOCUS       DATLEE010000014         3150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_13636, whole genome shotgun sequence.
ACCESSION   DATLEE010000014 DATLEE010000000
VERSION     DATLEE010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13636"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VM692_00340"
     CDS             complement(<1..215)
                     /locus_tag="VM692_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50633.1"
                     /translation="MFISKKHLSRRTVLRGVGAAVALPLLDAMIPAGTALAQTAARRK
                     PKLGFFYLPHGAVMDRWRPAATGREFEL"
     gene            complement(229..2610)
                     /locus_tag="VM692_00345"
     CDS             complement(229..2610)
                     /locus_tag="VM692_00345"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ50634.1"
                     /translation="MTRDYATSLRRGLAACFAAALASCGGAPSPEDLRAQGDEQWPFV
                     ERYCTECHNDTDLTADIAFDKLSPDDVAAHADVFERAVRKLRGRLMPPPGGLEPSTAE
                     TDAFVAWLEATLDAAAPAPGPGYVTPHRMNRTEYANAIRDLLALEVDPATLLPVDGAE
                     HGFDNIANALTVSPSFIDQFLSAARNLSAQAVGNAHPRASGTPYTIGNARGQQFHVDG
                     LPLGTRGGALIEHYFPADGEYRLNIGDLVTGLWGYNQEHKATVIAVLDGRKFFELDIG
                     GGQDLKELDQIGAPAVDKINARLKNIPFTATAGVHRLGVTFLHRSFAESDRQLYSQVP
                     GGGQDTVLTLNLVEVFGPVNATGLSSTPSRDKIFVCYPKSEAAQKPCANEIVATLARE
                     AFRGAFNDEDLPRLMRLYDEGAAHGGFEDGVEYALAGVLAHPKFLYRFEPKPGSAEPG
                     AAYPLSSVELASRLSFFLWSSIPDAELLELAAADKLAEPAVLEQQVARMLADPRAETL
                     ASNFAYQWLGLGELASLAPDPSLFADVDRNIRDYFVQESTLFVDSVFRSDASVLELLT
                     ADYTFVNETLARHYGLNDVRGQHFRRVELDDPNRFGLLGKGGVLLASSYPNRTSPVLR
                     GQWLLENLLGTPPASPPPNVEALVETVEGGQASTVRARLEAHRANPSCNACHGVIDPL
                     GFALENFDAVGRWRVIDREARAPIDASGVLVDGTPVTGPVELRKALLARPDQFVQTLT
                     EKLMTYGLGRSLEYTDMPTVRRIVREAAEDDYRFSDVVWGIVNSTQFRMKGGA"
     gene            complement(2582..>3150)
                     /locus_tag="VM692_00350"
     CDS             complement(2582..>3150)
                     /locus_tag="VM692_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50635.1"
                     /translation="GSEVWVRWSDKKYPPLPAAARPKTTVYDPGRLQFGSRAVDQGWA
                     RDAHTDGDLFLHFPDENVLVAGGFVSNWTWPIIDWWTGGWTVGMLNGFDRLLEVANER
                     TRVVPAVGPVMTFAELKTQHAMYLTVIERIHGMLIKALAVDEVLAQKPTAEFDARYGD
                     PTQFVTLAFESTWGHLRDAHDTRLRNIA"
BASE COUNT          519 a         1066 c         1074 g          491 t
ORIGIN      
        1 agctcgaact cgcggcccgt cgcggccggt cgccaccgat ccatgaccgc gccatgcggc
       61 aggtagaaaa acccgagctt cggcttccgt cgagcggccg tctgggccaa cgccgtgccg
      121 gcgggaatca tcgcgtcgag cagcggcagc gcaacggcgg cgccgacccc ccgcaagacc
      181 gtgcggcgtg acaggtgctt cttgctgatg aacatccgcg aagccccctc aggcgccgcc
      241 tttcatccga aactgcgtgc tattcacgat accccaaacg acgtccgaaa accggtagtc
      301 gtcctcggcc gcttcgcgca cgatgcggcg caccgtcggc atgtcggtgt attcgagtga
      361 ccttccgagc ccgtaagtca ttaatttttc ggtcaaagtc tggacgaact ggtcgggacg
      421 cgcgagcagc gccttgcgca gctcgacggg acccgtgacg ggcgtgccat cgacgagcac
      481 gcccgacgcg tcgatcggcg cccgcgcttc gcgatcgatg acgcgccagc ggccgacggc
      541 gtcgaagttc tcgagcgcga agccgagcgg atcgatgacg ccgtgacacg cgttgcagga
      601 cggattcgcg cgatgcgcct cgagccgcgc gcgcacggtg ctggcctgcc ctccttcgac
      661 ggtctcgacg agcgcttcga cgttcggcgg cggcgatgcc ggcggagtgc cgagcaagtt
      721 ctcgagcagc cactgaccgc gcagcaccgg cgacgtacgg ttcggatacg acgacgcaag
      781 cagcacgccg cctttgccga gcaggccgaa gcggttcgga tcgtcgagct cgacgcggcg
      841 gaagtgctgg ccgcgcacgt cgttcaagcc gtagtgacgc gccagcgtct cgttgacgaa
      901 cgtgtagtcg gcggtcagca attcgagcac gctggcatcc gaccggaaca cgctgtcgac
      961 gaacaacgtc gactcctgca cgaagtaatc gcggatgttg cggtcgacgt cggcgaacaa
     1021 tgacggatcc ggcgcgagac ttgcgagctc accgagaccg agccactggt acgcgaagtt
     1081 cgaggcgagc gtctcggcgc gcggatccgc gagcatgcgc gcgacttgct gctcgagcac
     1141 ggcgggctct gcgagcttgt cggcagccgc cagctcgagc agctcggcgt ccgggatcga
     1201 gctccacaag aagaacgaca gccgcgacgc gagctcgacg ctcgacagcg gatacgcggc
     1261 accgggctcc gcgctgccgg gcttcggctc gaagcgatac aagaacttcg gatgcgcgag
     1321 cacgccggcg agcgcgtact cgacgccgtc ctcgaagccg ccgtgcgcgg cgccttcgtc
     1381 gtagagccgc atgagccgcg gcaggtcctc gtcgttgaac gcgccgcgga acgcctcgcg
     1441 cgcgagcgtc gccacgatct cgttcgcgca cggcttctgc gcggcttcgg acttgggata
     1501 gcagacgaag atcttgtcac ggctgggcgt gctcgagagc cccgtcgcgt tcacgggccc
     1561 gaacacctcg acgagattca gcgtcagcac cgtgtcctgg ccgccgcccg gcacttgcga
     1621 gtagagctgg cggtcggact cggcgaacga acgatgcagg aacgtgacgc cgagccggtg
     1681 cacgccggcc gtcgccgtga acggaatgtt tttcaggcgc gcgttgatct tgtcgacggc
     1741 cggtgcgccg atctggtcga gctctttgag gtcctggccg ccgccgatat cgagctcgaa
     1801 gaacttgcgg ccgtcgagca cggcaatgac ggtcgccttg tgctcctggt tgtagcccca
     1861 gagccccgtg acgagatcgc cgatgttgag ccggtattcg ccgtcggccg ggaaatagtg
     1921 ctcgatcagc gccccgccgc gcgtgccgag cggcaggccg tcgacgtgaa actgctggcc
     1981 gcgcgcgttg ccgatcgtgt acggcgtgcc cgaggcgcgc ggatgcgcgt tgccgacggc
     2041 ttgcgcgctc aagttgcgcg ccgcgctcaa gaactggtcg atgaacgacg gggacaccgt
     2101 caacgcgttc gcgatgttgt cgaagccgtg ctcggcgccg tccacgggca gcaaggtcgc
     2161 ggggtcgacc tcgagtgcga gcagatcccg gatggcgttc gcgtactcgg tgcgattcat
     2221 gcgatgcggc gtgacgtaac cgggcccggg agccggcgcg gccgcatcga gcgtcgcctc
     2281 gagccacgcg acgaacgcat ccgtctcggc cgtgctcggc tcgaggccgc ccggcggcgg
     2341 catcaggcgc ccgcgcagct tgcgcacggc gcgctcgaag acgtcggcgt gcgcggcaac
     2401 gtcgtcgggc gacagcttgt cgaacgcgat gtcggccgtc aggtccgtat cgttgtggca
     2461 ctcggtgcaa taacgctcga cgaatggcca ctgctcgtcg ccctgcgcgc gtaagtcctc
     2521 gggggacggc gcgccgccgc aactcgcgag cgcggcggcg aagcacgcgg cgagcccgcg
     2581 cctaagcgat gttgcgtagt cgcgtgtcat gcgcgtcccg taaatgaccc cacgtgctct
     2641 cgaacgcgag cgtcacgaat tgcgtcggat cgccgtagcg cgcgtcgaac tcggccgtcg
     2701 gcttctgcgc gagcacctca tcgacggcga gcgccttgat cagcatgccg tggatgcgct
     2761 cgatgaccgt cagatacatc gcgtgctgcg tcttgagctc ggcgaaggtc atgacgggac
     2821 ccacggccgg cacgacgcgc gtgcgctcgt tcgcgacctc gagcaaccga tcgaagccgt
     2881 tcaacatgcc gacggtccag ccgcccgtcc accagtcgat gatcggccac gtccagttcg
     2941 agacgaaccc gccggcgacg agcacgttct cgtcggggaa atgcaggaac aagtcgccgt
     3001 cggtgtgcgc atcgcgcgcc caaccctgat cgacggcgcg cgagccgaac tgcaggcggc
     3061 ccgggtcgta gaccgtcgtc tttggccgcg ccgcagcggg caacggcgga tacttcttgt
     3121 ccgaccagcg cacccacact tcgctgccga
//
LOCUS       DATLEE010000015         3458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10441120, whole genome shotgun sequence.
ACCESSION   DATLEE010000015 DATLEE010000000
VERSION     DATLEE010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10441120"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VM692_00355"
     CDS             complement(<1..1066)
                     /locus_tag="VM692_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUQ50636.1"
                     /translation="MTGLLRRASLRFYLRHPWQLGLAIAGISLGVGVYVGVSLANDSA
                     ARAFDVAAGEVRGAITHRILPLGDSLDERLYRELLSYDGSLQATPFIEAEVGIEGRPE
                     LRVPLLGIEPLAGSGAVRAAQGASSQGARGARLLVEPNTVLVSREIADELGVDAGATL
                     GLAIGSRSVAVQVVGVVPTTGADVRVEPPIIADIATAQELLQSFGRISRIDLALTERQ
                     ARDLEARPLSDAILVPAENERSTFRELTTAFRTNLTALGLLALVVGMFLIYGTMAFAI
                     LQRTQALGILRTLGVSRTEILQTILWETAGIAIVATALGLVLGHVLAMGLVGLVLQTI
                     GDLSFNAAVDGVAPSPWIYVQ"
     gene            complement(1063..1743)
                     /locus_tag="VM692_00360"
     CDS             complement(1063..1743)
                     /locus_tag="VM692_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ50637.1"
                     /translation="MNAVTPAWIELENVSKRYGSQPVLDAANLQVARGQMVAVMGRSG
                     SGKSTLLRLIGGLEAADNGVVRVDGTDLTALTETERARRRRHGLGFVFQSFNLIPTLT
                     VAENVELPLALNDVAPADVRRRSRALLDELGLAGCADRFPEDISGGEQQRVAIARAVI
                     HEPKLVLADEPTGNLDAETAQHVFELLRRTCRERDATLIVATHSPDVAAQATRVLRIR
                     AGRIEDAA"
     gene            1924..3456
                     /locus_tag="VM692_00365"
     CDS             1924..3456
                     /locus_tag="VM692_00365"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HUQ50638.1"
                     /translation="MITPELRKKIESCHSLPSPPGVATKIIELANDPEADIARIADVL
                     ALDPAITAKILRIANSPMYARQRKTENLKQALMVIGLNATISLALSFSLLKSWQASDQ
                     AGGLDYPLYWRRALLGATASHVLARVIGVKDGEELFLACLIQDIGVMALDHVVPELYA
                     GLGPEQGRQAALIQRETERLGVDHAAVSGWLLEKWQFPERTQQVVAASHDPDRLDKQS
                     ANAVFARCVGLASMIAELFLDTSGARGFAELAQRAQAYFEIDKEGLGELLAEIGRMIP
                     DAESIFETEILSVGRSDQILAEAREALMLRNLQALRDVDQLKTSAESLEERTKRHEES
                     NKRDPLTNLFNRSYLDHYLRDAFQISNKSETPLSIAFADLDKFKSVNDTYGHAMGDQI
                     LVTTANILKANVRGADVVARYGGEEFILVFPHTEYPLLKTICERIVRAFRETRHDVGT
                     KRDLNVTISIGMATHNDGRQFKSVEELLKAADKALYTAKLQGRNRSVPFDLVANSQLA
                     YM"
BASE COUNT          626 a         1152 c         1126 g          554 t
ORIGIN      
        1 cctgcacgta aatccaaggt gacggcgcga cgccgtctac agccgcgttg aacgacagat
       61 cgccgatcgt ctgcagcacg agaccgacga gccccatcgc gagcacgtgg ccgagcacca
      121 atccgagcgc cgtcgcgacg atggcgatgc cggccgtctc ccagagaatc gtctgcaaaa
      181 tctcggtgcg tgagaccccg agtgtgcgca ggattccgag cgcctgcgtc cgctgcaaga
      241 ttgcgaacgc catggtgccg tagatcagga acatgccgac gacgagcgcc aacaggccga
      301 gcgcggtcag attcgtgcga aaagccgtcg tgagctcacg gaacgtgctg cgctcgttct
      361 ctgccgggac caaaatggcg tcgctcagcg gccgagcttc gagatcgcgc gcctgccgtt
      421 ccgtcagcgc gaggtcgatg cggctgattc gcccgaagct ctgcagcagc tcctgcgccg
      481 tcgcgatgtc ggcgatgatc ggcggctcga ctctgacgtc ggcgcccgtc gtcggcacga
      541 cgccgaccac ctgtacggcg acgctgcgcg agccaatagc gagcccgagc gtagcgccgg
      601 cgtcgacgcc gagctcgtcg gcgatttccc tcgataccag gaccgtgttt ggctctacga
      661 gcagccgcgc tcctcgagct ccctgcgacg acgctccctg cgctgcgcgc acggcgccgc
      721 tgccggccag cggctcgatg ccgaggagcg gcacgcgcag ctccggtcgg ccctcgatgc
      781 cgacctcggc ttcgatgaag ggcgtcgctt gcaagctgcc gtcgtacgac agcagctctc
      841 gatagaggcg ctcgtcgagg ctgtcgccga gcggcagaat gcggtgcgtg atcgcgccgc
      901 ggacctcgcc ggccgcgacg tcgaacgcgc gcgcagcact gtcgttcgcg aggctgacgc
      961 cgacgtagac gccgacgccg agactgatac cggcgatcgc gagcccgagc tgccacgggt
     1021 gccgtagata aaaacgcagc gacgcgcgcc gcagcaagcc tgtcacgccg catcctcgat
     1081 gcggcccgcg cgaattctca gcacgcgtgt cgcctgcgcc gcgacgtcgg ggctgtgcgt
     1141 ggcgacgatc agcgtcgcgt cgcgctcgcg gcaggtgcgc cgcagcagct cgaagacgtg
     1201 ttgggccgtc tcggcgtcga gattcccggt cggctcgtcg gcgagaacga gcttcggctc
     1261 gtggatcacc gcgcgcgcga tggcgacgcg ctgttgctcg ccgcccgaga tgtcctcggg
     1321 gaatcggtcc gcgcagcccg cgagcccgag ctcgtcgagt aatgcgcggc tgcggcgccg
     1381 cacgtccgcc ggcgcaacgt cgttcaacgc caacggcagc tcgacgtttt ccgcgacggt
     1441 caacgttgga atgagattga acgactgaaa cacgaagccg agcccatgcc gccgacggcg
     1501 ggcccgctcg gtttccgtga gcgccgtaag atcggtgccg tcgacgcgca cgacgccgtt
     1561 atcggcggcc tcgagcccgc cgatcagccg cagcagcgtc gacttgccgg agccgctgcg
     1621 gcccatgacg gcgaccattt gcccgcgcgc tacttgcagg ttcgccgcgt ccaaaacggg
     1681 ctgcgagccg tagcgtttgc tgacgttctc gagctcgatc caggccggtg tgacggcgtt
     1741 catgaaaacg atcctacgat gccgcgcatg ttgcgtggcg ctccagtcaa aaagccgctc
     1801 ggcgttcgtt atgtcaagcg ctcttaagtt cccgagcgtc cggccgacgt atgcctagag
     1861 aaaacgactg gccgctcgga tatgcgcggc cgtcgttcga gcgatcagaa agagaggccg
     1921 gctttgatta ctcctgaact ccgcaaaaag atcgaaagct gtcacagtct gccgagcccg
     1981 cccggagtcg cgacgaaaat catcgagctg gcgaacgatc ccgaggcgga catcgcccgg
     2041 atcgcggatg tgctggcgct cgatcccgcg atcaccgcga agattttgcg catcgcgaat
     2101 tccccgatgt acgcgcgcca gcgcaagacc gagaacttga agcaagcgtt gatggtgatc
     2161 ggcctcaacg caacgatctc gttggctctg tcgttctcgc tgctgaagtc gtggcaagcg
     2221 tcggaccagg ccggcggcct cgactatccc ctgtactggc gccgtgcgtt gctcggcgcg
     2281 acggcgagcc acgtcctggc tcgcgtcatc ggcgtcaagg acggcgaaga gctgttcctc
     2341 gcgtgcctga tccaggacat cggcgtcatg gcgctcgatc acgtcgtgcc ggagctctat
     2401 gccgggctcg ggcccgagca agggcggcag gccgcgctga tacagcgcga gaccgagcgc
     2461 ctcggcgtcg atcacgccgc cgtcagcggc tggctgctcg aaaaatggca gttcccggag
     2521 cgcacgcagc aagtcgtggc cgccagtcac gatcccgacc ggctcgacaa gcagagcgcg
     2581 aatgccgtgt tcgcacggtg cgtcgggctc gcgagcatga tcgcggagct gtttctcgac
     2641 acgagcggcg cgcgcggctt cgccgagctc gcgcaacgcg cgcaggctta cttcgagatc
     2701 gacaaggaag ggctcggcga gctgttggcc gagatcgggc gaatgatccc cgacgccgag
     2761 agcatcttcg agacggagat tctgtcggtc ggtcgttcgg accagatcct tgccgaggcg
     2821 cgcgaagcgt tgatgctgcg caacctgcag gccttacgcg acgtcgacca gttgaagacg
     2881 agcgcggagt cgctcgagga gcggacgaag cgccacgagg agtcgaacaa gcgcgacccg
     2941 ttgacgaatc tattcaaccg ctcctacctc gaccactatc tgcgcgacgc gtttcagatc
     3001 agcaacaaga gcgagacgcc gcttagcatc gcgtttgccg atctcgacaa gttcaagtcc
     3061 gtcaacgaca cctacggtca cgcgatgggc gaccagatcc tggttacgac cgcgaatatt
     3121 ttgaaagcga acgtccgcgg cgccgacgtc gtggctcgct acggcggcga agagttcatt
     3181 ctcgtcttcc cgcataccga gtacccactg ctcaagacca tctgcgagcg catcgtgcgc
     3241 gcgttccgcg agaccaggca cgacgtcggg accaagcgcg atctcaacgt cacgatctcg
     3301 atcggcatgg cgacgcacaa cgacggccgc caattcaaga gcgtcgagga gctgctgaaa
     3361 gccgcggaca aggcgctcta cacggcgaag ctgcaagggc gcaaccgcag cgttccgttc
     3421 gacctcgtgg cgaacagcca gctcgcgtac atgtagcc
//
LOCUS       DATLEE010000016         4981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_760626, whole genome shotgun sequence.
ACCESSION   DATLEE010000016 DATLEE010000000
VERSION     DATLEE010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760626"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1230)
                     /gene="carA"
                     /locus_tag="VM692_00370"
     CDS             complement(82..1230)
                     /gene="carA"
                     /locus_tag="VM692_00370"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HUQ50639.1"
                     /translation="MAEPAILALEDGTVFKGVSVGAEGRTLGEVVFNTAMTGYQEILT
                     DPSYFRQIVTLTYPHIGNVGTNSDDFESTKIYAAGLIIRDLSALVSNFRAEGGLEQFL
                     RRGKTVAIADIDTRQLTRILRDKGAQAGCIVTGAKAKPDEAVKAARKFPGLKGMDLAK
                     LVTTKQSYQWNQGTIWPERQSVYTKRVASFHVVAFDYGIKRNILRLISDQDCRLTVVP
                     ATTKAEEVMALMPDGVFLSNGPGDPEPCDYAIEAIRTLVDKGVPTFGICLGHQLLGLA
                     SGAKTEKMKFGHHGANHPVVELDSGRVMITSQNHGFAVQENTLPANLRATHRSLFDGT
                     LQGLERTDRPAFSFQGHPEASPGPADLKPLFQRFTASMKTYRHRAQAV"
     gene            complement(1372..2181)
                     /gene="dapB"
                     /locus_tag="VM692_00375"
     CDS             complement(1372..2181)
                     /gene="dapB"
                     /locus_tag="VM692_00375"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HUQ50640.1"
                     /translation="MLKVAVLGAAGRMGRSVLSCILEADDLKLVGAVTETGDALLGRD
                     AGELVGGAQAGVPLTDERSQGLNTAQVAVDFTLPTVTEANLRACAEQGTALVIGTTGL
                     EARQLRAMEKAAHDIPIVYARNMSVGVSVFMELVGRAAKALGDAYDVEIAETHHRHKV
                     DAPSGTALALGERVAAARGRKLADLAVYARQGQTGPRVPGTIGFAVTRGGSVVGEHAV
                     RFIGAEEEVEFSHRAADRKTFARGALHAARWAAGQAPGLYTMADVLGLSRD"
     gene            complement(2185..3321)
                     /gene="dnaJ"
                     /locus_tag="VM692_00380"
     CDS             complement(2185..3321)
                     /gene="dnaJ"
                     /locus_tag="VM692_00380"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HUQ50641.1"
                     /translation="MAKRDYYEVLGVARDASEADVKKAYRRLAMKHHPDRNPGNPEAE
                     AHFKEAKEAYEALSDKDKRAAYDRFGHAGLQGHQGPGGGFGGGPDAFSDIFGDVFGDI
                     FGAGRRGGRNQVFRGADLRYELELTLEQAVAGDSVTLDVPAQVECTRCTGKGAEPGSQ
                     PITCKTCGGAGQVRMAQGFFSIQQTCPNCGGAGATIDKPCRDCNGRGRVRKNKTLSVK
                     VPPGVDNGDRIRLAREGEAGRNGGPAGDLYVDISVQQHPIFTREGQNLSCEVPVSFAT
                     ATLGGSVEVPTLDGNVTLKVPAETQSGSLFRLRGKGVRSVRDSGLGDLFCRVQIETPV
                     NLTADQREKLRAFDAAIQNEGGRHSPRARSWFDGVKEFFERMGA"
     gene            complement(3401..>4981)
                     /gene="dnaK"
                     /locus_tag="VM692_00385"
     CDS             complement(3401..>4981)
                     /gene="dnaK"
                     /locus_tag="VM692_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HUQ50642.1"
                     /translation="QVLIKMKKTAEDYLGEKVTEAVITVPAYFNDSQRQATKDAGKIA
                     GLEVKRIINEPTAAALAYGLDKKRGDRKIAVYDLGGGTFDISIIEIAEVDGEHQFEVL
                     ATNGDTFLGGEDFDKRIIDYLAKEFQKESGVDIRRDPLAMQRLKEASEKAKIELSSRQ
                     QTEVNLPYITADASGPKHLNINLTRAKLESLVEDLIEKTIAPCKTALNDAGLKVSEVD
                     DVILVGGQTRMPKVQETVQTLFGKEPRKDVNPDEAVAVGAAIQAGVLAGEVKDVLLLD
                     VTPLSLGIETLGGVMTKLIDKNTTIPTKANQTFSTAEDNQTAVTIHVLQGERGRAGDN
                     KSLGRFDLADLPPAPRGMPQVEVTFDIDANGILHVSAKDKASGQEQKIVIKASSGLSE
                     DEIKRMVGDAEAHAAEDAKFRELADARNQADALVHQTEKSLRELGDKVSGEERGKIEA
                     ALADVKEAIKSDNKTVIETKLRTLGEASASMAQRLYANQGGASGGAPGGEEAADGGGD
                     NVVDAEFEEVKDDKRKGG"
BASE COUNT          745 a         1690 c         1556 g          990 t
ORIGIN      
        1 tcaggacgct ttcgagatcg tcgcgacgtg gcattagcta atccgattcc ttctgattga
       61 tatgtaactg cgtgattttg cttaaactgc ttgcgcccga tgacggtagg ttttcatcga
      121 agcggtgaag cgctgaaaca gcggcttcaa gtccgcgggc ccggggctcg cttcgggatg
      181 cccctggaac gagaaggcag gccgatcggt gcgctcgaga ccctgcagcg tgccgtcgaa
      241 tagtgaccgg tgcgtcgctc gcaagttcgc cggcagcgtg ttctcctgga cggcgaaacc
      301 atggttctgg ctcgtgatca tgacgcggcc ggaatcgagc tcgacgaccg ggtgattcgc
      361 gccgtggtgg ccgaacttca tcttctccgt cttggcgccg ctggcgagac cgagcagctg
      421 gtgcccgagg caaatgccga acgtcggcac tcctttgtcg acgagcgtgc ggatcgcttc
      481 gattgcgtaa tcgcagggct ccggatcgcc gggcccgttc gacaagaaaa cgccatcggg
      541 catcagcgcc atgacttcct cggccttcgt cgtcgccggc accacggtca ggcggcagtc
      601 ctggtcggag atcaatcgca aaatattgcg cttgatgccg taatcgaacg cgacgacgtg
      661 gaagctcgcg acgcgtttcg tgtacacgct ctgccgttcc ggccagatcg tgccttgatt
      721 ccactgatac gactgcttcg tcgtcacgag cttcgccaag tccatgccct tcaggcccgg
      781 aaacttgcgc gcggctttca cggcctcgtc gggttttgct ttcgcgcccg tgacgatgca
      841 gcccgcctgc gcgcctttgt cgcgcaggat gcgcgtcagc tgtcgagtgt cgatgtcggc
      901 gatcgcgacc gtcttgccgc gtcgcaggaa ttgctcgagc ccgccttcgg cgcggaagtt
      961 gctgacgagc gccgacaggt cgcgaatgat gagcccggcc gcgtagatct tcgtcgattc
     1021 gaagtcgtcg gagttcgtgc cgacgttgcc gatgtgtggg tacgtcagcg tgacgatttg
     1081 gcggaagtag gacgggtccg tcaggatctc ttgatagccc gtcattgcgg tgttgaacac
     1141 cacttcgccg agcgttcggc cttcggcgcc gaccgataca cccttgaaca ccgtgccatc
     1201 ttcgagcgct agtatcgccg gctctgccac gctgagctcc tacaaaaatt cggcttgcgt
     1261 aaagacgcgg gatgggctgc aagcccggca gccaatcccg tgttgatgaa cggggaaact
     1321 caggcggcaa ttgtagctcg acctttcgcg atcgatctac cgggtgccgt atcagtcgcg
     1381 cgatagtccg agcacgtcgg ccatcgtgta gagacccggc gcctggccgg ctgcccaacg
     1441 agcggcatgc agcgcgccgc gagcgaacgt cttgcgatcg gcggcgcggt gcgagaactc
     1501 gacttcctct tcagcgccga tgaagcgcac ggcgtgctcg ccgacgacgc tgccaccgcg
     1561 cgtcacggcg aagccgatcg tgccgggcac tcgcggaccc gtctggccct ggcgcgcgta
     1621 gacggcgaga tcggcgagct tgcgcccacg cgcggcggcg acgcgctcgc cgagcgccag
     1681 agccgtgccc gacggggcgt cgaccttgtg ccgatggtga gtttcggcga tctcgacgtc
     1741 gtaggcgtcg ccgagagctt tggcggcgcg gccgacgagc tccatgaaga cgctcacgcc
     1801 cacgctcatg ttgcgcgcgt agacgatcgg aatatcgtgc gcggccttct ccatcgcgcg
     1861 cagctgccgc gcctccaggc ccgtagtgcc gatgacgagc gccgtgccct gctccgcgca
     1921 agcgcgcaga ttggcctccg tgaccgtcgg caacgtgaag tcgacggcga cctgcgccgt
     1981 gttcaacccc tgactgcgtt catccgtcag cggcacgccg gcttgcgcgc cgccgacgag
     2041 ctcgccggca tcgcgaccga gcagagcgtc gccggtctcc gtcacagcgc cgacgagctt
     2101 caggtcgtcg gcttcaagga tgcagctcag cacggagcgt cccatgcggc ccgcggcccc
     2161 gagaacggct accttcagca acggctatgc tcccatccgc tcgaagaact ctttgacgcc
     2221 gtcgaaccac gaacgagccc gcgggctgtg cctgccgcct tcgttctgga tcgccgcgtc
     2281 gaaggcacgc agcttctccc gctgatccgc cgtcaaattg actggcgtct cgatctgcac
     2341 gcggcagaac aggtcgccga gaccgctatc gcgcaccgag cgtacgcctt tgccgcgcag
     2401 ccgaaacagg ctcccggact gcgtctcggc tggcaccttg agcgtcacgt taccatcgag
     2461 cgtcggcacc tcgacactgc cgcccaacgt ggccgtcgcg aagctcacgg gcacctcgca
     2521 gctcaagttc tggccttcgc gcgtgaagat cgggtgctgt tgcacggaga tgtcgacgta
     2581 gagatctccg gccggcccgc cattccgccc ggcctcgccc tcgcgcgcga ggcggatgcg
     2641 gtcgccattg tcgacgccgg gcggcacctt cacggacaac gtcttgttct tgcgcacgcg
     2701 gccgcggccg ttgcaatcgc ggcacggctt gtcgatcgtc gcgccggctc cgccgcagtt
     2761 cgggcacgtt tgctggatcg agaagaaacc ctgcgccatg cggacttggc cggcaccgcc
     2821 gcaagtcttg cacgtgatcg gctgcgagcc cggctcggcg cccttgcccg tgcagcgcgt
     2881 gcactccact tgcgccggca cgtcgagagt gaccgagtcg ccggcgaccg cttgctcgag
     2941 cgtcagctcg agctcgtagc gcaggtcagc gccgcgaaag acctgattgc gcccgccgcg
     3001 tcgtcccgcg ccgaaaatgt cgccgaatac gtcgccgaag atatcgctga acgcgtcggg
     3061 tccaccgccg aatccgccgc ccgggccctg atggccctgg agcccggcgt gaccgaagcg
     3121 atcgtaagcg gcgcgcttgt ccttgtcgga cagcgcttcg tacgcctcct tcgcctcttt
     3181 gaagtgcgcc tcggcttccg ggttgcccgg attgcgatcg ggatgatgct tcatcgctag
     3241 gcgtcgatac gccttcttga cgtcggcttc cgaggcgtcg cgcgcgacgc ccagcacttc
     3301 gtagtaatcc cgcttcgcca tccccgtcct cgatagcagg agagcacgcc tgagcgtgct
     3361 ctcctagtag tcacatcggc cagcgttgcc gctgctcggc ttagccgcct ttgcgcttgt
     3421 cgtccttgac ctcttcgaac tccgcgtcga cgacgttgtc gccgccgccg tccgccgcct
     3481 cctcgccgcc gggcgctccg cctgacgcgc caccttggtt cgcgtacaac cgctgcgcca
     3541 tgctcgccga ggcctcgccg agcgtccgca acttcgtctc gatcacggtc ttgttgtcgc
     3601 tcttgatcgc ttctttgacg tcggcgagcg cggcttcgat tttcccacgc tcctcgcctg
     3661 agactttgtc gccgagctcg cgcaacgact tctcggtctg gtgcaccaac gcgtccgcct
     3721 ggttgcgcgc gtctgcgagc tcacggaact tggcgtcctc ggccgcatgg gcttcggcgt
     3781 cgccgaccat tcgcttgatc tcgtcctcgc tcaagcccga cgaggctttg atgacgatct
     3841 tctgctcttg gccagacgcc ttgtccttgg ccgacacgtg gaggatgccg ttcgcgtcga
     3901 tgtcgaacgt gacctcgacc tgcggcatgc cgcgcggcgc cggcggcagg tccgcgagat
     3961 cgaaccggcc cagcgacttg ttgtcaccgg cgcggccgcg ctcgccctgc aacacgtgaa
     4021 tcgtcacggc ggtttggttg tcctccgccg tcgagaaggt ctggttggcc ttcgtcggaa
     4081 tcgtcgtatt cttgtcgatg agcttcgtca tgacgccgcc taacgtctcg atgccgagcg
     4141 acagcggcgt gacgtcgagc aacaggacgt ccttgacctc gcccgcgagc acgccggctt
     4201 ggatcgccgc accgacggcg acggcctcgt ctgggttcac atccttgcgc ggctccttgc
     4261 cgaacagcgt ctgcacggtc tcttggacct tcggcatgcg cgtctggccg cccacgagga
     4321 tcacgtcgtc gacttccgac accttcaagc ccgcgtcatt caacgcggtc ttgcacggcg
     4381 ctattgtttt ctcgatcaaa tcctcgacga gcgactcgag cttggcgcga gtcagattga
     4441 tgttcaggtg cttcgggccc gaggcgtcgg ccgtgatgta cggcagattg acctcggtct
     4501 gctgacgcga cgagagctcg atcttcgcct tctcggacgc ttccttgagg cgctgcatcg
     4561 cgagcggatc gcgccggatg tcgacgccgc tctccttttg gaactccttc gcgaggtagt
     4621 cgatgatgcg cttgtcgaag tcctcgccgc cgaggaacgt gtcgccgttc gtcgcgagca
     4681 cctcgaactg gtgctcgccg tcgacctcgg cgatctcgat gatggaaatg tcgaacgtgc
     4741 cgccgcccaa atcgtagacg gcgatcttgc ggtcgccgcg cttcttgtcg agaccgtaag
     4801 cgagcgccgc cgccgtcggc tcgttgatga tgcgcttgac ctcgaggccc gcgatcttgc
     4861 ctgcgtcctt cgtggcctgg cgctgcgagt cgttgaagta cgccggcacc gtgatcacgg
     4921 cctcggtcac cttctcgccg aggtaatcct cggcggtctt cttcatcttg atgagcacct
     4981 g
//
LOCUS       DATLEE010000017         4271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8206410, whole genome shotgun sequence.
ACCESSION   DATLEE010000017 DATLEE010000000
VERSION     DATLEE010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8206410"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1552
                     /locus_tag="VM692_00390"
     CDS             44..1552
                     /locus_tag="VM692_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUQ50643.1"
                     /translation="MSLAIAAQPAPSTGVAAFLRRPARLLIGGEWVSSAGKEQIPVID
                     PATGAEVASIADANRADVDKAVAAAREAFERGPWSAMLPAHREALIWKLADLIDAHAD
                     ELAELESIDNGKTKQMASIVDVPGARNYFRYMAGWATKIEGSTIDVSVGGLKNARINA
                     YTRREPVGVVAQIIPWNFPLVMAAWKLGPALAAGCTCILKPAEQTPLTALRLGDLIIE
                     AGFPPGVVNILTGYGETVGAALVTHPGVDKIAFTGSTLVGKAINKAATDTLKRVSLEL
                     GGKSPVIVLPDADVDTVIGGAAGAIFFNAGQVCAAGSRLYVHRKIFDRVVEGVGGAAQ
                     SIRLGPGLDPSTQMGPLVSREQQERVIGYIKSGRDEGASVVAGGDTPSHPGFYVKPTV
                     MADVNRSMRVVQEEIFGPVLVAQRFDDLDEVAALANDTPYGLSASIWSNDLKAVHRLI
                     PKVKAGTVWVNTHGPVDANMPFGGYKHSGLGREHGRVGIEMYTEVKSVCMLV"
     gene            1570..2286
                     /locus_tag="VM692_00395"
     CDS             1570..2286
                     /locus_tag="VM692_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUQ50644.1"
                     /translation="MSSVDPNGKEAVRRREPAQRASAGTGSVASLVRVLDESRLKPKA
                     KEQEVLDVATEYFLQHGYRGASINAMARSSGISKESIYRYFSSKQQLFEAVIGRELIE
                     YRRSLHRLDATLRSMDLRAALVTVAETILGIITTDRTLALRRLIFDEATRSPEVGQHY
                     YKIGPGQAYSVLENVLETHAHHSDFDIPTLARHFTGLLSWRITLERQCAVRQEPNAQE
                     IAELAATLVEDFMKAFLKPK"
     gene            2394..3695
                     /locus_tag="VM692_00400"
     CDS             2394..3695
                     /locus_tag="VM692_00400"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ50645.1"
                     /translation="MTELLIRNAYVLTMDAAVGDIAGGDVHVRAGAIAAVGRNLTAPG
                     AEVIDGAGMLVLPGFVETHWHLWTTLLRSLSGDRQEHGYFPTSRTVGTFYTPDDMYVA
                     GRLAATEAVHSGITFLHDWCHNVRAPEYAEAALRALAEVGVRARFSYGSPTGASNDAT
                     IDLKDLQRLRDGWSNYGNGGLLTLGLAWRGAASESTMRDYEVAKELGLPISVHANNFQ
                     TSAGGIQQLADRNLLTQGMQVIHAVWCTPEEIRALAANRVNVSVSPYSEMRIGFGFPI
                     VADLTAAGVTVGLSVDTPALSGNADMFAIMKAIQNIENGRAVNEFKVTARRALELATI
                     DGARSMGVGDIVGSLTPGKRADLIMVDTRAVNLAVMTEPAHMLVEAAQPANVDTVIID
                     GRIVKRGGRLTRIDVGDVVDSASRALTGVRRRSGWNWPSQI"
     gene            complement(3686..>4271)
                     /locus_tag="VM692_00405"
     CDS             complement(3686..>4271)
                     /locus_tag="VM692_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUQ50646.1"
                     /translation="EQGCKEFVEKAAAQLGGLDILVNNVGGMIPGTLESLTAETWAKV
                     LNVNLMAAVYATKYAVPFIKKSRAGRVLNVSGVTGKQLMVGALTTTIPNAGIIAFSKL
                     MAGELGPAGITVNNICPGFTKVESWGPRAEAMAKVRNVTSDQVRAGIANQTLLGRWAE
                     PEEIGNVAAFLVSDKNGYMTGTTVEVCGGFNRYI"
BASE COUNT          748 a         1382 c         1406 g          735 t
ORIGIN      
        1 tcgatcccag tcgtcctcat aacaaagact atcggggaac tgcatgagtc tcgccattgc
       61 tgcccagcca gcgccgtcga ccggcgtcgc ggccttcttg cggcggccgg ctaggctgtt
      121 gatcggcggc gaatgggtgt cgtcggccgg caaagagcaa atccccgtca tcgaccccgc
      181 caccggtgcc gaggttgcgt cgatcgcgga tgcgaatcga gcagacgtgg acaaggccgt
      241 cgccgccgct cgcgaggcgt tcgagcgcgg cccatggtct gcgatgcttc cggcgcatcg
      301 cgaagcgctg atctggaagc tcgccgacct catcgatgcg cacgccgacg agttggccga
      361 gctcgagagc atcgacaacg gcaagacgaa gcagatggcg agcatcgtcg acgtgccggg
      421 agcgcgtaac tactttcgtt acatggccgg ttgggccacg aagatcgaag gttcgacgat
      481 cgatgtttcg gtaggcggcc tcaagaacgc acgcatcaat gcctacacgc gccgcgagcc
      541 cgtgggcgtc gtggcgcaga tcatcccgtg gaattttccg ctcgtcatgg ctgcgtggaa
      601 gctcgggccc gcgctcgcgg ccggctgcac ctgcattctg aagcccgccg agcagacgcc
      661 gctgacggcg ctgaggctcg gggatctcat catcgaggcc ggcttcccgc ccggcgtcgt
      721 caatatcctc acgggctacg gcgagaccgt tggcgccgcg ctcgtcacgc accccggcgt
      781 cgacaagatc gcgttcacgg gctcgacgct cgtcggcaaa gccatcaaca aggctgcgac
      841 ggacacgctg aagcgcgtct cgttggagct cggcggcaag tcgcccgtca ttgttcttcc
      901 cgacgccgac gtcgacaccg tcatcggcgg cgcagctggc gcgatctttt tcaacgcggg
      961 ccaggtctgc gcggccggct cgcgcctata cgtgcatcgg aagattttcg atcgcgtggt
     1021 cgaaggcgtc ggcggcgcgg cgcaatccat tcggcttggc cccggcctcg acccaagcac
     1081 gcagatgggg ccgctggtct cgcgcgagca gcaagagcga gtgatcggct acatcaagtc
     1141 cgggcgcgac gagggcgcgt cggtcgtcgc cggcggcgat acgccgtcgc atcccggctt
     1201 ctacgtcaag ccgaccgtca tggccgacgt caatcgctcg atgcgcgtcg tgcaggaaga
     1261 aatctttggg cctgtgctcg tcgcgcagcg tttcgacgat ctcgacgagg tcgcggctct
     1321 ggcaaacgac acgccgtacg gcttgagcgc aagcatctgg tcgaacgatt tgaaggccgt
     1381 gcatcggctg attccgaagg tcaaagccgg cacggtctgg gtcaacacgc acgggcctgt
     1441 cgacgcgaac atgccgttcg gcgggtacaa gcattcagga ctcggccgtg agcacggtcg
     1501 cgtcggtatc gagatgtaca cggaagtgaa atccgtgtgc atgctggtct agtggagtca
     1561 ggagtgaaga tgagttcagt cgatcccaat ggcaaggaag cagtgcgccg gcgcgagcct
     1621 gcgcaacgcg cgagcgcggg cacgggcagc gtcgccagcc tcgtgcgtgt cctcgacgaa
     1681 agccggttga agccgaaggc caaggagcaa gaggtcctcg acgtcgcaac cgaatatttt
     1741 ctgcagcacg gctatcgagg cgcgagcatc aacgcgatgg cgcgcagctc gggcatttcc
     1801 aaggagtcga tctaccgcta cttcagcagc aagcagcagc tgttcgaagc cgtcatcggc
     1861 cgcgagctca tcgaatatcg gcgcagcttg caccgcttgg acgcgacgct gcgcagcatg
     1921 gacctgcgcg cagcgctcgt gacggtcgcc gagacgattc tcggcatcat cacgacggat
     1981 cggacgctgg cgctgcgccg gttgatcttc gacgaagcga cgcgctcgcc ggaggtgggt
     2041 cagcactact acaagatcgg gcccggccaa gcgtattcgg tgctcgagaa cgtgctcgag
     2101 acgcacgcac accacagcga tttcgacatc ccgacgttgg cgcggcattt cacggggctc
     2161 ttgtcgtggc gcatcacgct ggagcgccag tgcgcggttc gccaagagcc gaacgcgcaa
     2221 gagatcgcgg agctcgccgc gacgctcgtc gaggacttca tgaaggcgtt tctcaagccg
     2281 aagtgaacgc gacgcggtgg aatcggcggg atgtattggg cggagtcctc gccgccgggg
     2341 ccatcggcag cacgcccctc aagctcgggt tcgcgcagcc gcgggctgac tccgtgaccg
     2401 agcttctgat ccgcaatgcc tatgtgctga cgatggatgc cgccgtcggc gacatcgccg
     2461 gcggcgacgt gcacgtccgc gccggcgcca tcgcggccgt cggccgtaat ctcacggcgc
     2521 cgggtgccga ggtcatcgac ggcgccggca tgttggtttt gccggggttc gtcgagacgc
     2581 actggcatct ctggacgaca ttgttgcgta gcctctcggg cgaccggcaa gagcacggtt
     2641 actttccgac gtcgcgcacc gtcggcacgt tctacacgcc cgacgacatg tacgtcgccg
     2701 gcagactcgc cgcgaccgag gcggtgcact ccggtatcac gtttctgcac gactggtgcc
     2761 acaacgtgcg cgcccccgag tacgccgaag cggcgttacg cgcgcttgcc gaagtcggcg
     2821 ttcgcgcgcg cttctcgtac ggctcgccga cgggtgccag caacgacgcg acgatcgacc
     2881 tcaaggattt gcagcgcctg cgcgacggct ggagcaacta tggcaacggc ggtctgctga
     2941 cgctcggcct cgcgtggcgc ggcgctgcgt cggaatcgac gatgcgcgac tacgaagtcg
     3001 ccaaagagct cggcttgccg atttccgtgc acgccaataa ttttcagacg agcgccggcg
     3061 gcatccaaca gctcgcggac cgcaacttgc tgacacaagg catgcaggtc atccatgccg
     3121 tctggtgcac gcccgaagag attcgagcgc tcgccgccaa tcgcgtcaac gtcagtgtgt
     3181 cgccgtactc ggagatgcgc atcggcttcg gttttcccat cgttgcggac cttacggcgg
     3241 ccggtgtgac ggtcggtctg tccgtcgaca cgccggcgct gtccggtaac gccgacatgt
     3301 tcgcgatcat gaaggcgata cagaacatcg agaacgggcg agccgtcaac gagttcaagg
     3361 tcacggcgcg ccgcgcgctc gagctcgcga cgatcgacgg cgcgcgctcg atgggtgtcg
     3421 gcgacatcgt cggctcgttg acgcccggca agcgggccga cctgatcatg gtcgacactc
     3481 gcgccgtgaa tctcgccgtc atgacggagc ccgcgcacat gctcgtcgag gcggcgcagc
     3541 ccgccaacgt ggacaccgtc atcatcgacg gccgcatcgt caagcgcggc ggacgcctga
     3601 cgagaatcga cgttggtgac gtcgtcgact cggcaagccg agcgctgacg ggcgtacgcc
     3661 ggcgctccgg ttggaactgg ccgagtcaga tataacggtt gaaaccgccg caaacctcga
     3721 ccgtcgttcc cgtcatgtag ccgttcttgt ccgagacgag gaacgccgcg acgttgccga
     3781 tctcttccgg ctccgcccaa cggccgagca gcgtctgatt ggcgatgcca gcgcggacct
     3841 ggtccgacgt gacgttgcgg accttggcca tcgcttcggc acgcgggccc caggactcga
     3901 ccttggtaaa gcccgggcaa atgttgttga ccgtgatccc ggcaggaccg agctcgccgg
     3961 ccatgagctt cgagaacgcg atgatgccgg cgttcggaat cgtcgtcgtc agagcgccga
     4021 ccatgagctg cttgcccgtg acacccgaga cgttgagcac gcggcccgcg cggctcttct
     4081 tgatgaaggg caccgcgtat ttcgtcgcgt agacggcagc catgagattg acgttcagca
     4141 ccttggccca cgtctccgcc gtgaggcttt cgagcgtgcc cgggatcatg ccgccgacgt
     4201 tgttgacgag gatatcgagg ccgccgagct gcgcagccgc cttctcgacg aactccttgc
     4261 agccttgctc g
//
LOCUS       DATLEE010000018         4139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1510038, whole genome shotgun sequence.
ACCESSION   DATLEE010000018 DATLEE010000000
VERSION     DATLEE010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510038"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VM692_00410"
     CDS             complement(<1..188)
                     /locus_tag="VM692_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50647.1"
                     /translation="MHTLRRPRLALASFVASIVCCGAVSAQMDFSGEWPVVLSQDNQD
                     NPHLGEYVGIPLSREAIRR"
     gene            627..2066
                     /locus_tag="VM692_00415"
     CDS             627..2066
                     /locus_tag="VM692_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50648.1"
                     /translation="MQSSADSLLAEIEAFCRRSGIAESTFGRQSVNDGKLCVRLRNGK
                     DVTLETAAKIRGFIEKQPAVQKLNGTENYEQSNNKGETMSGNTKRKASSGPKNKAPAT
                     ADGSADRPFRFYDNRQKYLAFVNTTNEKWKVAQRATRELELLKPNPPALRLFDAGMGD
                     ATVLSHIMRAMHRRYPIMPFFIVGKEISLEDVRLSLEKLPDRFIEHPASVIVITNLYY
                     AEAPWLRPRDVQMAAALNWKQVALEGSNSSDYGEQLRSIDSFLVDGWQVKASEKTGNP
                     MYVRPSVLVIYRKDHEFLLDNVIPKQGQVGGNYDLVVASQPWRARMSAEFKAKKVLGP
                     LVRALGPAGRLLAVQSSGDDPALEMVNEVWPDEKPFKVNRHELIKALRDELGREARDF
                     NFVSGSDAKSIFRYEMHTLPSEVQQSIGTSTLFAAWNAASYVAQIEDERIENAMREGD
                     HYLQGTAKILKKHKGLWFNDESFVVSRKQ"
     gene            2081..2989
                     /locus_tag="VM692_00420"
     CDS             2081..2989
                     /locus_tag="VM692_00420"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HUQ50649.1"
                     /translation="MANDELKQHIAAFLSDYSIEITPHDTGKLDAIRAELAPGTCVYV
                     AHPPGTPIDDIVALAGQVQKLGLTATPHIIARKLESRDQLERALARCQELGIHNALCV
                     AGDITADKPAFNSSLEVLQTGLFKKYGFREVGVAGHPEGSKAIGEERVEQALRGKAEF
                     AKTAGFNLYFATQFGFDPQPFIDWEAETSAKGINLPIHVGMPGPASLRQLAKFAMLCG
                     VGASMRMLTSRTSAMANLLSAQAPDDMVTALARYKGSNPQSRFKKVHFFAFGGVVKTA
                     RWANAVISGRFVLNNQATGFRVEDVN"
     gene            3026..>4139
                     /gene="metK"
                     /locus_tag="VM692_00425"
     CDS             3026..>4139
                     /gene="metK"
                     /locus_tag="VM692_00425"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HUQ50650.1"
                     /translation="MSKSYLFTSESVSEGHPDKIADQVSDAILDAILAQEPRSRVACE
                     TMTKTGMVIVAGEITTNAVIEVEHIVRQTVLEIGYDHSDKGFDGNTCAVLNGLGKQSQ
                     DIAQGVDRKSPEEQGAGDQGLMFGYATIETDSLMPAPIIFSHRLVEKQAEVRKNGKLP
                     WLRPDAKSQCTFRYENDKPVGIDAVVLSTQHSPDISLKDLQEAIMEEVIKPVLPAKWL
                     NKNTQYHINPTGRFVIGGPMGDCGLTGRKIIVDSYGGFARHGGGAFSGKDPSKVDRSA
                     AYAARYVAKNVVAAGLAERCEVQVSYAIGVAKPTSVTVETFGTNKVPEEKIIAAVNKN
                     FDLRPYGIVKMLDLLKPIYKKTASYGHFGREGFSWEK"
BASE COUNT          857 a         1299 c         1299 g          684 t
ORIGIN      
        1 cggcggatgg cttcgcgcga gagcgggatg ccgacgtact cgccgaggtg cgggttgtcc
       61 tggttgtctt gcgacaacac gacgggccac tcgcccgaaa aatccatctg cgcactgacc
      121 gcgccgcaac agacgatgct ggcgacgaag ctcgccagcg cgagccgagg tcggcggagt
      181 gtgtgcataa gccccctgcg ttgcgcgcac gaattcacgc gcaaaatcgc aagtcatggt
      241 agccgcggca acaccccccc agcaagggaa tggccgcgta cggaaacgcg gcgcgcgccg
      301 cgtcatttct atagattctt cggcgtcgcc gaaccgttcg gtcgtgtgcg agcgattcgg
      361 agcacctcga tcgcgagcaa aagatcgggt gagctcaccg ttgtgccgtg cgaggcgctc
      421 gcgtagcgtc gccgggccgc tcgcacgcag cacactttgc cggactccga cacgttctcg
      481 atcgtgcatg ggattctgcc caccacgatg cgagctaaca aagatgggca aaaatattgc
      541 attggttttt gcgtaggcgt atggttgcgc cggctgcaaa aagcaaaact cggcagccca
      601 tctctctccc tggggaacac taagtgatgc agagctcggc ggactcgctg ttggcggaaa
      661 tcgaagcgtt ttgccgccga agtggtatcg ccgagtcgac cttcggccgg cagtcggtca
      721 acgacggcaa gctctgtgtc cggttacgca acggcaagga tgtcactctc gagacggcgg
      781 ccaagatccg cgggttcatc gagaagcaac cggcggtaca gaagctgaac ggcaccgaaa
      841 actacgagca gagcaacaac aagggcgaaa ccatgagtgg taacaccaag cgtaaagcct
      901 cctcaggtcc gaagaacaag gctcccgcaa cggccgacgg ctccgccgat cggccgtttc
      961 gtttttacga caaccggcag aagtacctcg cgttcgtcaa cacgacgaac gagaagtgga
     1021 aagtcgcgca acgcgccacg cgcgagctcg agctgctgaa gccgaatccg ccggcgctgc
     1081 ggctgttcga cgccggcatg ggcgacgcca cggtgctgtc gcacatcatg cgcgcgatgc
     1141 atcgccgcta cccgatcatg ccgttcttca tcgtcggcaa agaaatcagc ctcgaggacg
     1201 tgcgcctgtc gctcgagaag ctgccggacc gcttcatcga gcacccggcc agcgtcatcg
     1261 tcatcacgaa cctctattac gccgaggccc cgtggctgcg gccgcgcgac gtgcaaatgg
     1321 ccgcggcgct gaactggaag caagttgcgc tcgagggcag caattcttcc gactacggcg
     1381 agcagctgcg cagtatcgac tcgttcctcg tcgacggctg gcaggtcaag gccagcgaga
     1441 agacgggcaa cccgatgtac gtgcggccct cggtgctcgt catctaccgc aaggaccacg
     1501 agttcctgct cgacaacgtg atcccgaagc agggccaggt cggcggcaac tacgatctcg
     1561 tcgtcgcctc gcagccttgg cgcgcgcgca tgagtgccga gttcaaagcc aagaaggtgc
     1621 tcgggccgct cgtgcgcgct ttgggccccg ccggccgttt gttggccgtg caatcgtcgg
     1681 gcgacgaccc ggcgctcgag atggtcaacg aagtttggcc cgacgagaag ccgttcaagg
     1741 tcaaccggca cgagctcatc aaggcgttgc gcgacgagct cggccgtgaa gcgcgcgact
     1801 tcaatttcgt ctcgggctcc gacgcgaagt cgatcttccg ctacgagatg catacgctgc
     1861 cgagcgaggt gcaacagagc atcggcacgt cgactctgtt cgccgcgtgg aacgcagcga
     1921 gctacgtggc gcagatcgaa gacgagcgca tcgagaacgc gatgcgcgag ggcgatcact
     1981 acctgcaagg caccgcgaag atcctcaaga agcacaaggg tctttggttc aacgacgaga
     2041 gcttcgtcgt ctcgcgcaag cagtaacgtc gagcagcacg atggcaaacg acgagctcaa
     2101 gcaacacatc gccgcttttt taagcgacta ttcgatcgag atcacgccgc acgacacggg
     2161 caagctcgat gcgatccgcg ccgagctcgc gccgggcacc tgcgtttatg tcgcgcatcc
     2221 gcccggcacg ccgatcgacg acatcgtcgc gctggccggc caggtgcaaa agctcggcct
     2281 cacggcgaca ccgcacatca tcgcgcgcaa gctcgagagc cgcgaccagc tcgagcgggc
     2341 gctcgcgcgc tgccaagagc tgggcatcca caatgcgctt tgcgttgcgg gcgacatcac
     2401 ggccgacaag cctgccttca acagcagcct cgaggtccta cagacggggc tcttcaagaa
     2461 atacggtttc cgcgaggtcg gcgtcgccgg ccatcccgag ggcagcaagg ccatcggcga
     2521 agagcgcgtc gagcaggcgc tgcgcggcaa ggccgagttc gcaaagacgg ccggcttcaa
     2581 cctgtatttc gcgacccagt tcggcttcga tccgcaaccg ttcatcgatt gggaagccga
     2641 gacctcggcc aagggcatca acctgccgat ccacgtcggc atgccgggcc ccgcgtccct
     2701 ccgtcagctc gcgaagttcg cgatgctttg cggcgtaggt gcctccatgc gtatgcttac
     2761 gtcgcgcacg agcgcgatgg cgaatctcct gagcgcgcag gcaccggacg acatggtcac
     2821 ggcgctcgcg cggtacaagg gctcgaatcc gcagtcgcgg ttcaagaagg tccatttctt
     2881 tgccttcggc ggggtcgtca agaccgcgcg ttgggctaat gcggtgattt ctgggcggtt
     2941 cgtcttgaac aaccaggcga ctggtttccg tgttgaagac gtcaactgaa tttttttgat
     3001 agaccgaaaa gtagggtaga aaaccatgag caagtcgtat ctgtttacgt cggagtccgt
     3061 gtcggaagga catccggata agatcgccga tcaggtctcg gacgcgatcc tcgacgcaat
     3121 cctcgcgcaa gagccgcgca gccgcgtcgc ctgcgagacg atgacgaaga ccggcatggt
     3181 gatcgtcgcc ggcgagatca cgacgaatgc cgtgatcgag gtcgagcaca tcgtgcgtca
     3241 aaccgtcctc gagatcggtt acgaccactc ggataaaggt ttcgacggca acacgtgcgc
     3301 cgttttgaac ggcctcggca agcagtcgca agacatcgcc cagggcgtcg accgtaagag
     3361 ccccgaagag cagggcgccg gcgaccaggg cctcatgttc ggctacgcga caattgagac
     3421 ggacagtctc atgccggcgc cgatcatctt ctcgcaccgc ttggtcgaga agcaggccga
     3481 agtgcgcaag aacggcaagc tgccatggct gcgcccggac gcgaagagcc agtgcacgtt
     3541 ccgctacgag aacgacaagc ccgtcggcat cgacgcggtc gtcctctcga cgcagcacag
     3601 ccccgacatc tccctgaagg acctgcaaga ggccatcatg gaagaggtca tcaagccggt
     3661 gctgccggcg aagtggctca acaagaacac gcagtaccac atcaatccga ccggccggtt
     3721 cgtcatcggc ggcccgatgg gcgactgcgg tctgacgggc cgtaagatca tcgtcgacag
     3781 ctacggcggc tttgcccgcc acggcggcgg cgcgttctcg ggcaaggatc cttcgaaggt
     3841 cgaccgctcg gctgcctacg ccgcgcgcta cgtcgcgaag aacgtcgtgg cggcgggcct
     3901 cgcggagcgt tgcgaagtcc aggtatcgta cgcgatcggg gtcgcgaagc cgacgtcggt
     3961 gacggtcgag accttcggca cgaacaaggt gcccgaggag aagatcatcg ccgcggtcaa
     4021 caagaacttc gacctgcgtc cgtacggcat cgtcaagatg ctcgatctgc tgaagcccat
     4081 ctacaagaag acggcctcgt acggccactt cggccgcgaa ggcttcagct gggagaaga
//
LOCUS       DATLEE010000019         3049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1512651, whole genome shotgun sequence.
ACCESSION   DATLEE010000019 DATLEE010000000
VERSION     DATLEE010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512651"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VM692_00430"
     CDS             <1..228
                     /locus_tag="VM692_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50651.1"
                     /translation="ALPPMRPLIVVAPGQDQFWVFDAFVGYRMPNRRGLLSLNADNLL
                     DKGFQFQDTDPTNPSLFPERLISLRFTLAFD"
     gene            336..791
                     /locus_tag="VM692_00435"
     CDS             336..791
                     /locus_tag="VM692_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HUQ50652.1"
                     /translation="MPEAIPAEADARSEIAALLHEQFARLTRQLRTLDLPQGMTPERL
                     SALSVIDKRGPISVTALAEKEMVRPATMSRMVSALVEDGLVKRSDDKTDGRGVLVSAT
                     PKGRRTYQRAQEQRLQHFAEVLDSLSDDQIAAMRNLTAALERLTLLLDK"
     gene            complement(796..2295)
                     /locus_tag="VM692_00440"
     CDS             complement(796..2295)
                     /locus_tag="VM692_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ50653.1"
                     /translation="MDAPNAAAGFDFAILEASTVVAAIVVAGDGRVVAANARMRRFLG
                     IADSGPYGQLPDYLVDASASEAWRDAARARCSIEVELRGFGGATQVFRGDLLPHGEGS
                     NRRIVGVFVEGDERKALRAAAQHSARMEALGSLTAGVAHDFNNLLTVLVGNLYLIGEE
                     VRERPRVFEKLKAARDAGKRGTELIKQLMTFARREELTVGVIDPQNVIADLLPLLRRA
                     LGARITLETKLQANVGAVRASVAQLESVVVNLAVNARDAIEGKGTISIEVIPADVTEQ
                     EATLRGLARGGRYIAVTVRDTGSGIPPEALSRVFEPFFSTKRERGGTGLGLSMVRWFA
                     EHAGGAAAIESVVGQGTTVTLMLPRIQPEQADSIEMTMPLSTLPTGNERVVVLALDEA
                     LRATIHQTLEVLGYRVRVASGAEDLLAAVAAEQTELLMVDGLGRGDADILIRARAAQP
                     KLRIIATADPSRGAERIAGVGIASLAKPFTLADLAGTVRRTLDAAADAL"
     gene            2546..>3049
                     /locus_tag="VM692_00445"
     CDS             2546..>3049
                     /locus_tag="VM692_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUQ50654.1"
                     /translation="MSDTPLTKTIASTATLKIANIHSLDLFYTPLEERFERITRLARR
                     SLQAPVAATSLLNEDKQWFKSAAGWGISELPRDHAICRLTVEAGEILMIGDTLDDPRV
                     AKLPVVATAPRFRSYAGHPLLDEHGACVGTFCVFDLKPREFSPADRQTLTDLAALAQR
                     ELQSDQLS"
BASE COUNT          550 a         1025 c          933 g          541 t
ORIGIN      
        1 gcgcttccgc cgatgcggcc gcttatcgtg gtagcgcccg ggcaggacca gttctgggtc
       61 ttcgatgcct tcgtcggcta ccgcatgccc aatcgtcgcg gcttgttgtc tctgaacgca
      121 gacaacttgc tggacaaggg cttccaattc caggataccg atccgacgaa tccgagcttg
      181 tttcccgagc gcttgatctc gctgcggttc acgttggcct tcgactgagg gcgcgggaat
      241 gcgatcccaa aaccgataca gatgacttgc cagcctctac aatttctcgg agtgacgtca
      301 tcccgcagaa taatcacaat gaggtagtcc aactcatgcc agaagcaatt cccgcagagg
      361 cggatgcgcg ctccgagatc gccgccctgc tgcacgaaca attcgcgcga ctgacgcgac
      421 agctacgcac gctggaccta cctcagggaa tgacaccgga gcgtttgagc gccttgtccg
      481 tcattgacaa gcgcggcccc atctccgtca cagcgttggc cgagaaggaa atggtccgcc
      541 ccgcgacgat gtcccgtatg gtgtcggcgc tcgtcgaaga cggcctcgtc aagcgcagcg
      601 acgacaagac cgatggccgc ggcgtgctgg tcagcgcgac gccgaagggc cggcgtacgt
      661 atcagcgcgc acaagagcag cgcctgcagc atttcgcaga ggtactcgat tcgctgagcg
      721 acgaccagat cgccgccatg cggaacctga cggccgcgct cgagcggctg acattgctgc
      781 tcgacaagta acgggctaga gagcgtctgc tgcggcgtcg agagttcgcc gcaccgttcc
      841 cgcaagatcg gccaacgtga acggcttcgc gagcgacgcg atgccgacac ctgcaatgcg
      901 ctccgctccg cgactcgggt ccgccgtcgc gatgatccgc agcttcggct gcgccgcgcg
      961 cgctcgtatc aagatgtcgg cgtcgccgcg gcctaagccg tcgaccatca acaactccgt
     1021 ctgctcggcg gccacggccg ccagtagatc ctcggcgccc gaggccaccc tgacgcgata
     1081 gccgaggacc tcgagcgttt ggtggatggt cgcgcgcagc gcctcgtcca gggcgagcac
     1141 gacgacgcgc tcgttgcccg taggcagcgt cgacaacggc atcgtcatct cgatggagtc
     1201 cgcctgctca ggctgaatac gcggcagcat caacgtcacg gtggtgcctt ggccgacgac
     1261 gctttcgatg gctgcggcgc cgcccgcgtg ctctgcgaac cagcgcacca tgctgagccc
     1321 gagaccggta ccaccgcgct cgcgtttcgt cgagaaaaac ggctcgaata cgcggctcag
     1381 ggcttcggga gggatgccgc tgccggtatc gcgtaccgtg acggcgatgt aacgcccacc
     1441 gcgtgcgagc ccacgcagcg ttgcttcttg ctcagtcacg tcagccggta tgacttcgat
     1501 cgagatggtg ccttttccct cgatggcgtc gcgtgcgttg accgccagat tgacgactac
     1561 gctttcgagc tgtgccacgc tggcccgtac ggcgcctacg tttgcttgaa gcttcgtctc
     1621 gagcgtaata cgcgcgccca aggcgcggcg cagcagtggc aagagatctg cgatcacgtt
     1681 ctgcggatcg atgacgccga ccgtgagctc ctcgcggcgc gcaaacgtca tgagctgctt
     1741 gatgagctcg gtgccgcgtt tgccggcgtc gcgcgccgct ttgagcttct cgaacacgcg
     1801 cggccgctcg cgcacctctt cgccgatcag atacagattg ccgaccagga ccgtcagcaa
     1861 gttgttgaag tcatgcgcga cgcctgccgt cagactgccg agcgcttcca tgcgagcgct
     1921 gtgctgagcg gccgcgcgca gcgccttgcg ctcgtcgccc tcgacgaaaa cgccgacgat
     1981 tctgcggttg gacccctcac cgtgcggcag caaatcgccg cggaacactt gcgtcgctcc
     2041 gccgaagccg cgcagctcga cctcgattga acaacgcgcg cgggcagcgt cgcgccacgc
     2101 ctccgaagcc gaggcatcga cgaggtagtc cgggagctgc ccgtacgggc ccgagtcagc
     2161 gatcccgagg aagcgccgca tgcgcgcgtt cgcagcgacg acgcggccat cgcctgcaac
     2221 gacgatagcc gcgacgacgg tgctcgcttc gaggattgca aaatcgaagc cggcggccgc
     2281 attcggagcg tccatccggc tcgttggctg tggcatcgac tcggttccaa aagaacggtg
     2341 ggctctggcc caagcgcaag tgtatcgaaa ggctaactac tgcccctagc gcagctattg
     2401 actggtgtca cactttgacg actcgcccac cgttgcaacg agtgtcacgg ccggcccaag
     2461 cgagacacaa ctcacacgtc ggccgcggca cgcgcggtac agtaagcgct gcctcaagcg
     2521 ccagagatcc tggtccggaa tactcgtgag cgacacgccc ctaacgaaga ccatcgcgag
     2581 cacagcgacg ctcaagatcg ccaatatcca ttcgctcgac ttgttctata cgccgctcga
     2641 ggaacgcttc gagcgcatca cgcgtctcgc tcgtcgctcg ctacaggcgc ccgttgcggc
     2701 gacctcccta ctgaacgagg acaagcaatg gttcaagtcg gcggctggtt gggggatctc
     2761 cgaactgccg cgtgatcatg caatctgccg gctcaccgtc gaagcgggcg aaatcctcat
     2821 gatcggcgat acgctcgacg acccgcgcgt tgccaagctg ccggtcgtcg cgactgcccc
     2881 gcgctttcgt tcctacgccg gtcatccgct gctcgacgag catggcgctt gcgtcggcac
     2941 gttttgcgtt ttcgatttga agccgcgcga attttcgccg gctgatcggc agacgttgac
     3001 cgacctcgcc gcactggcgc aacgcgagct ccagtccgac cagctgtcg
//
LOCUS       DATLEE010000020         4490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6718040, whole genome shotgun sequence.
ACCESSION   DATLEE010000020 DATLEE010000000
VERSION     DATLEE010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6718040"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VM692_00450"
     CDS             <1..329
                     /locus_tag="VM692_00450"
                     /inference="COORDINATES: protein motif:HMM:NF019253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HUQ50655.1"
                     /translation="DAEGGQPVDPHGTYVDGSAISGPADLRQVLLEHSELFVQTFAEK
                     LLTYALGRALEPTDMPAVREIVRRAKRDDYRLSALVLGVVQSVPMQMKVKMPAVSAES
                     QARVAN"
     gene            339..1673
                     /locus_tag="VM692_00455"
     CDS             339..1673
                     /locus_tag="VM692_00455"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ50656.1"
                     /translation="MFVTKKHLSRRTLLRSAGAAIALPLLDSMVPAATALAQTAATPK
                     SRLGCIYIPHGATMDKWTPAATGSQLELSEILAPLEPFKKQLCVVSGLAHAGVAPWTG
                     EDTGGAENHVRAAAVFLSGAHPIKGDRAIVGPSVDQLAAAHIGQDTPLPSVELSIEEV
                     GLNCDANFTCAYRNTLAWKSATLPLPMENNPQVAFERLFGDGGTDAERAARRTEARSL
                     LDSVIEQVSSLRTALPAADKARLADYLDEVREVERRVLNIDSRLSTGVELPEAPVGVP
                     DTFEEHLNVMFDLQVLAYRTEISRISTLMLARENSNAVYPGSGVREGFHNASHHSNER
                     KNMEQFAVINRYHVKTLTYFLDKLRSTPDGDGTLLDHSMILYGSSLSDGNEHNFDPLP
                     VLLAGGASGQLQGGRHLQFPAHTPMSNLLLAMLHKLGAPVDKIGDSTEPLAI"
     gene            1788..2237
                     /locus_tag="VM692_00460"
     CDS             1788..2237
                     /locus_tag="VM692_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUQ50657.1"
                     /translation="MTRRVLQNVTRRDLIAVLAGVLLLGSFAIPAFNGYVERSRVARA
                     VSDIGTLSLQLYRWQKDTSTLPSSLAEAGLGADDPWGRPYVYRRAAGARQAELRKDDA
                     LEPLNSDFDLYSLGPDGESALALPAAPSHDDVVRARNGAYIGLAVNY"
     gene            complement(2231..3139)
                     /locus_tag="VM692_00465"
     CDS             complement(2231..3139)
                     /locus_tag="VM692_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ50658.1"
                     /translation="MKRALAAFVWLGIIGQALGQGSSEPTVRPYLWSAERPNSEVGEI
                     NVFRRFSSERTAAMREFYADVLGLPVLPETALGGGQMIRYPVGTSEVKLFPVAPSPAN
                     TAGPTDAVGVRLLTFFFADEAAVARRFTAHGLAAPTFARATARPGATVAALTQDPDGE
                     WVELVVVPGASAEALARFEIGIAVSDLAASRAFYGDVMGLEGKPPVRDELLGADTHAF
                     THGATTINLWQAAAAAPNDSATGGMQYIVWNVAAIDGVVRERGAKVDRPLSAPGQMRT
                     LWLSDPDGVSNYFAEFGGNDNRPVSQ"
     gene            complement(3192..>4490)
                     /gene="dacB"
                     /locus_tag="VM692_00470"
     CDS             complement(3192..>4490)
                     /gene="dacB"
                     /locus_tag="VM692_00470"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HUQ50659.1"
                     /translation="VVVQAVDDAKPLVSHLPDVARSPASVMKVVTTWSALEYLGPAFT
                     WPTEVYFLGPFDGHKLDGDLAIKGYGDPYLVVEEVWKLLRSLRRMGLTEITGDLVLDD
                     SHFRVDEPEPGAFDGQPYRTYNVVPNALLMNFKAVQFQFMADAQRARVIVATEPELAN
                     LKVDNNLTLVDGPCRGFQAGISFNHADLESLERVVLDGQFSRRCNVYGLARTVLQHDT
                     FAYGLFSSLWKEVGGSFSGKLRKAQVPADATPAFTWQSKPLGEVVRSINKNSNNVMTR
                     QLVYTLGAEAAGAPGTRANGIAAIRELLASRGLSIDSLMLQNGAGLSRDERASMQLLV
                     DILRAAYRSPYGPEFIASLSLGGLDGTTRSRFDASMPAAGVMHLKTGRLDHVSAVAGY
                     VRGANEKTYVVAVVMNSEDAHRGPGQELEEAVLRYTLSLR"
BASE COUNT          762 a         1507 c         1498 g          723 t
ORIGIN      
        1 gggatgctga gggcggccaa cccgtcgatc cgcacggcac gtatgtcgac ggcagcgcga
       61 tctcaggccc tgccgacttg cggcaggtgc tgctcgagca cagcgagctg ttcgtgcaga
      121 cgttcgctga gaagctgctg acctacgcgc tgggccgtgc gctcgagccg accgacatgc
      181 cggccgtgcg cgagatcgtg cggcgcgcga agcgcgacga ctatcggctt tcggcgctcg
      241 tgctcggcgt ggtgcagagc gtgccaatgc agatgaaggt caagatgccc gcggtcagcg
      301 cagaatcgca ggcacgagta gcgaactagg gcacacgaat gttcgtcacg aagaagcatc
      361 tgtctcgccg cacgctgttg cgcagcgccg gtgccgcgat tgcgctgccg ctgctcgact
      421 cgatggtccc cgccgctacc gcgcttgcgc agacggccgc cacacctaag agccggctcg
      481 gctgcatcta catcccgcat ggcgcgacga tggacaagtg gacgccggcc gcgaccggct
      541 cgcagctcga gctgtccgaa atcttggcgc cgctcgagcc gttcaagaag cagctctgcg
      601 tcgtcagcgg cctcgcgcat gccggcgttg cgccgtggac cggcgaggat acgggcggtg
      661 cggagaatca cgtgcgcgcc gccgccgtgt tcttgagcgg cgcgcacccg atcaagggcg
      721 accgagcaat cgtcgggccg agcgtcgacc agctcgcggc ggcccacatc ggccaagaca
      781 cgcccttgcc gtccgtcgag ctgtcgatcg aggaggtcgg gctcaactgc gacgcgaatt
      841 tcacgtgcgc ctaccgcaac acgctggcgt ggaagtccgc gaccttgccg ctgccgatgg
      901 agaacaatcc gcaggtcgcg ttcgagcgtc tgttcggcga cggcggcacg gacgccgagc
      961 gcgcggcgcg ccgcaccgag gcgcgcagct tgctcgactc cgtgatcgag caagtctcgt
     1021 cgctgcgcac ggccttgccg gccgccgaca aagcgcggct cgccgactac ttggacgagg
     1081 ttcgagaggt cgagcggcgc gtgctgaaca tcgattcgcg gctcagcacg ggcgtcgagc
     1141 tgcccgaggc gcccgtcggc gtgcccgaca cgttcgagga gcacttgaac gtgatgttcg
     1201 atctacaggt gctcgcttac cgcaccgaga tctcgcgcat ttcgacgttg atgctcgcgc
     1261 gcgagaacag caacgcggtc tatcctggca gcggcgtgcg cgaaggtttc cacaacgcgt
     1321 cgcaccactc gaacgagcgc aagaacatgg agcagttcgc cgtcatcaac cgctaccatg
     1381 tcaagacgct gacttatttc ttggacaagc tgcgctccac accggacggc gacggcacac
     1441 tgctcgacca ttcgatgatc ctctacggca gcagcctgag cgacggcaac gagcacaact
     1501 tcgatccgct gcccgtgctg ctcgcgggtg gcgcgtcggg tcagctgcaa ggcggacgtc
     1561 atttgcagtt ccccgcccac acgccgatgt cgaacctgct gctcgcgatg ctccataagc
     1621 tcggcgcgcc cgtcgacaag atcggtgaca gcacggagcc gctcgcgatc taagccgccg
     1681 cccgcttcgt cgtccgcgtg tcaagcgcgc gggagtgaaa cggttcacag caagcggccc
     1741 gcggttcgcc caatctcggt tccgcgattg gaacgggggg cgccgcagtg acacgacgag
     1801 tgctgcagaa cgtaacgaga cgggatctca tcgcagtgct tgccggagtg ctgctgcttg
     1861 gctcattcgc tattccggcg ttcaacggct acgtcgagcg ctcgcgagtc gctcgggcgg
     1921 tcagcgacat cggcacgctg agcttgcagc tctatcgctg gcaaaaggac acgagcacgt
     1981 tgccgtcgag cctcgctgaa gcgggcctcg gcgccgacga cccgtgggga cgaccgtacg
     2041 tgtatcggcg agccgccggc gcgcggcagg cagagctgcg caaggacgac gcgctcgagc
     2101 cgctgaactc ggacttcgat ctctatagct tggggcccga tggcgagtcg gcgctcgcgc
     2161 tgccggcggc gccgtcgcac gacgacgtcg tgcgcgcccg gaacggcgcc tacatcggcc
     2221 tggccgtcaa ctactgagac acggggcggt tgtcgttgcc cccgaactcc gcgaagtagt
     2281 tgctgacgcc gtcgggatcc gacagccaca gcgtgcgcat ctggccgggt gccgataacg
     2341 gccgatcgac cttggcgccg cgctcgcgca cgacgccatc gatcgccgcg acgttccaga
     2401 cgatgtactg catgccgccc gtggcagagt cgttcggcgc ggccgcggcc gcttgccaga
     2461 ggttgatcgt cgtcgcgccg tgcgtgaacg cgtgcgtgtc ggcgccgagt agctcgtcgc
     2521 gcaccggcgg cttcccctcg aggcccatca cgtcgccgta gaacgcgcgg ctcgcggcga
     2581 gatcggagac ggcaatgccg atctcgaaac gcgcaagcgc ctcggccgat gcgccgggca
     2641 ctacgacgag ctcgacccat tcgccgtccg gatcttgcgt caaggcggcg actgtggcgc
     2701 ccggccgcgc cgtcgcccgc gcgaacgtcg gcgccgccaa gccgtgcgcc gtgaagcgcc
     2761 gcgccacggc ggcctcgtcg gcgaaaaaga acgtcagcag gcgcaccccg acggcgtccg
     2821 tcggtccagc cgtgttcgcc ggcgacggcg cgacgggaaa cagcttcacc tcggatgtgc
     2881 ccacgggata acgaatcatt tgcccgccgc cgagcgccgt ctccggcagg accggtaagc
     2941 cgaggacgtc ggcgtagaac tcgcgcatcg cagccgtgcg ctccgacgag aaccgccgga
     3001 acacgttgat ctcgccaact tcggaattcg gccgctcggc gctccagaga taaggcctaa
     3061 cggtcggctc gctgctgcct tgtcccagag cctggccaat gatgccgagc catacgaacg
     3121 ctgccaaagc gcgcttcatc gtgaaccctc gtttggctac atcgagcata gccggtagat
     3181 cgccgcgggc cttagcgcag tgacaacgtg tagcgcagca cggcttcttc gagctcttgg
     3241 ccggggccgc ggtgcgcatc ctcgctgttc atgacgacgg cgacgacgta cgtcttctcg
     3301 ttggcgccgc gcacgtagcc agcgaccgcc gaaacgtgat cgagccgccc cgtcttcaga
     3361 tgcatgacgc cggccgccgg catcgacgcg tcgaagcggc tgcgcgtcgt accgtcgaga
     3421 ccgccgagcg acagcgaggc gatgaactcg ggtccgtacg ggctgcggta agccgcccgc
     3481 aagatgtcga ccaagagttg catcgaagcg cgctcgtcgc gcgacaagcc cgcgccgttc
     3541 tgcaacatca gggagtcgat gctcaagccg cggctcgcca acagctcgcg tatggcggca
     3601 ataccgttcg cgcgcgttcc cggggcgccc gcggcctcgg cgccgagcgt gtagacgagc
     3661 tgccgcgtca tgacgttgtt gctgttcttg ttgatgctgc ggacgacctc gccgagcggc
     3721 ttcgattgcc aagtgaacgc cggcgtcgcg tcggccggca cctgtgcctt gcgcaatttg
     3781 ccgctgaaag acccgccgac ttctttccac agcgacgaga acaacccgta ggcgaaggta
     3841 tcgtgctgca ggacggtgcg cgcgaggccg tagacgttgc agcggcgcga gaactgcccg
     3901 tcgagcacca cgcgctcgag cgattccaag tccgcgtggt tgaacgagat gccggcttga
     3961 aagccgcggc acggtccgtc gacgagcgtc aggttgttgt cgactttcag attcgcgagc
     4021 tcgggctccg tcgctacgat gacgcgggcg cgctgcgcat cggccatgaa ttggaactgc
     4081 acggccttga aattcatcag cagcgcgttc ggcacgacgt tgtaggtgcg gtacggctgc
     4141 ccgtcgaatg cgccgggctc gggctcgtct acgcggaagt gcgagtcgtc gagcacgaga
     4201 tcgcccgtga tctcggtcag ccccatgcgc cgcaacgatc gcaacagctt ccagacttcc
     4261 tcgacgacga ggtacggatc gccgtagccc ttgatggcga ggtcgccgtc gagcttgtgg
     4321 ccgtcgaacg gcccaaggaa ataaacctcg gtgggccagg tgaaggcggg gccgagatac
     4381 tcgagcgcgc tccacgtcgt gacgactttc atgaccgacg cggggctgcg cgccacgtcg
     4441 ggcagatgac tgacgagcgg tttcgcatcg tcgacggctt gcacgacgac
//
LOCUS       DATLEE010000021         2449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4495201, whole genome shotgun sequence.
ACCESSION   DATLEE010000021 DATLEE010000000
VERSION     DATLEE010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4495201"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1088
                     /locus_tag="VM692_00475"
     CDS             72..1088
                     /locus_tag="VM692_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ50660.1"
                     /translation="MADGTVRGRFVWHDLQTPNPAGAHDFYGNVLGWKKQAWDQDPSY
                     QMFAAPSGPLGGAVETRNATPQWLAYIGTKDVDEAIAAATALGGRVQTPATPLPNGGT
                     YAVLVDPQGATFGVHASSSGAPPEQAPQIGEFSWHELATNVAPNVAFGFYSSLFGWDE
                     ITQYDMGPMGMYLIFGRNGTQLGGMFDKGSEGKPGSAYWLGYVSVDDVEGTIERAKAA
                     RGSLLTGPMDVPGGDRIAQLMDPHGAFFALHMRAGTARPATKASPPPKASKPAKAVAP
                     KKKAPAPKKPSSKTRPAKKAAAKKKPTTARKAAKKKAPAKKPTSRKTAKKAKGKAKTR
                     KRGR"
     gene            complement(1108..1251)
                     /locus_tag="VM692_00480"
     CDS             complement(1108..1251)
                     /locus_tag="VM692_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50661.1"
                     /translation="MVAIDEDWSAVRFRRVEHIKTLSRPPELALSQEGKRKSSRPRRT
                     RRA"
     gene            complement(1468..>2449)
                     /locus_tag="VM692_00485"
     CDS             complement(1468..>2449)
                     /locus_tag="VM692_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HUQ50662.1"
                     /translation="FMAIMGLSLLLYVILDGYDLGIGMLLPFAGNDDKDVMIAAIGPF
                     WDANETWLVLGVGVLLIAFPAAHGLVLSSLYLPVTAMLIGLILRGVSFDFRVKADARQ
                     KRLWNRAFAAGSLLASLAQGWMLGSYVTGLTPGRINLAFAALIAVTLPALYLVLGAGW
                     LLVKTEGALFDRAARWGRAAVLPMGLALLAISIATPIVSPTIRDKWFTLPNFIGLMPI
                     PLATTVAFVAVVAVLRRVSSARPAYAWLVLANTVLMCVLAAIGLAYSIYPYVVLDRLT
                     VWEAAAAPGSLLFVLVGLAIVLPFTLAYTVFVYRVFRGKATALGYGSPSE"
BASE COUNT          502 a          821 c          802 g          324 t
ORIGIN      
        1 gaggacgatc agcactctcg agctcgacgc gaatcccgag ctccgtcgtt tcagccagat
       61 cggagaaatg catggcagat ggcactgttc gcggccgatt cgtttggcat gacttgcaga
      121 cgccgaaccc tgccggggcc cacgacttct acggtaacgt cctcggctgg aagaaacagg
      181 cctgggacca agacccctcc tatcagatgt tcgccgcgcc gagcggaccg cttggcggcg
      241 ccgtcgagac gcgcaacgcc acgccgcagt ggctggcgta catcggcacg aaagacgtcg
      301 acgaggccat tgcggcagcg acggccttgg gcgggcgagt gcaaacgccg gcgacccccc
      361 tgccgaacgg cgggacctac gccgtgctcg tggatccgca aggtgcaacc ttcggcgtgc
      421 atgcgtcgag ctcgggcgcc ccgcccgagc aggccccgca gatcggtgaa ttcagctggc
      481 acgagctcgc gacgaacgtc gcgcccaacg tcgcgttcgg tttttactct tcgttgttcg
      541 gctgggacga gatcacccag tacgacatgg gcccgatggg catgtatctg atcttcgggc
      601 gtaacggcac ccagctcggc ggcatgttcg acaaaggcag cgagggcaag ccgggcagcg
      661 cctactggct cggctacgtc agcgtcgacg acgtcgaggg cacgatcgag cgcgcgaaag
      721 cggcgcgcgg ctcgctgctg acgggcccga tggacgtgcc aggcggcgat cgcatcgcgc
      781 agctcatgga cccgcacggc gcgttcttcg cgctgcacat gcgggccggc acggcgaggc
      841 ctgcgacgaa ggcgtcgccg ccgccgaagg cgagcaagcc tgcaaaggcg gtcgcgccga
      901 agaagaaggc gccggcgcca aagaaaccga gttcgaagac gcggcccgcg aagaaggctg
      961 cggcgaagaa aaagcccacg actgcacgca aggcggcgaa gaaaaaggct ccggcgaaaa
     1021 aaccaacatc gcgcaagacc gccaagaaag ccaaaggcaa ggcgaagacc cgcaagcgcg
     1081 gacgctagtg cggtcgccct tttcggctca ggcgcgccgc gtgcgcctgg gtcgactgct
     1141 cttgcgcttg ccttcctgcg acagcgcgag ctcgggcggc cgcgacaacg tcttgatgtg
     1201 ctcgacgcgc cggaatcgca cggccgacca gtcttcgtcg atcgcaacca tgcgcacggg
     1261 ctcgaaaccg gcggcgccaa gagcggccca gccgttgtcg cgattgaact cgcagcggta
     1321 cttcttcgac gactgcttcg ggtaggcgaa ccagacgatc gcgcagcgct gcgagctcgg
     1381 gctcgaagct ctgcggcgcg tcgcagacga cgatctccgt gtgctctttc aggttcagct
     1441 ttttgaacac gggcgacata ggacagctca ctcactcggg ctgccgtagc cgagcgccgt
     1501 ggctttgccg cgaaacacgc gatagacgaa taccgtatag gccaacgtga acggcagcac
     1561 gatcgccaac ccgacgagca cgaacagcaa cgaccccgga gccgccgcgg cttcccaaac
     1621 cgtcagccgg tcgagcacga cgtacggata aatgctgtac gcgagcccga tcgccgcgag
     1681 cacgcacatc agcaccgtgt tcgcgagcac gagccaggcg taagccggtc gcgcgctcga
     1741 gacgcgccgc agcacggcca cgaccgcgac gaacgcgacc gtcgtcgcga gcggaatcgg
     1801 catgaggccg atgaagttcg gcaacgtgaa ccacttgtcg cggatcgtcg ggctgacgat
     1861 cggcgtggcg atcgagatgg ccagcagcgc gaggcccata ggcagcacgg cagcacggcc
     1921 ccagcgcgcc gcgcgatcga acagcgcgcc ctcggtcttc acgagcagcc agcccgcgcc
     1981 gagcacgaga tagagcgccg gtagcgtcac ggcgatcaac gcggcgaacg cgagattgat
     2041 gcgcccgggc gtgagccccg tgacgtagct gccgagcatc cagccctggg ccagcgaggc
     2101 cagcaacgaa cccgcggcga acgcgcgatt ccagaggcgc ttctgccgcg catccgcttt
     2161 gacgcgaaag tcgaacgaca cgccgcgcaa gatcaagccg atcaacatcg ccgtgaccgg
     2221 caagtacagc gacgacagca cgagcccatg cgcggccggg aatgcgatca gcagcacgcc
     2281 gacgccgagc acgagccacg tctcgttcgc gtcccaaaac gggccgatcg ccgcgatcat
     2341 gacgtccttg tcgtcgttgc ccgcgaacgg cagcagcatg ccgatcccta agtcatagcc
     2401 gtcgaggatc acgtacagca gcagcgacaa gcccatgatg gccatgaag
//
LOCUS       DATLEE010000022         8759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_770399, whole genome shotgun sequence.
ACCESSION   DATLEE010000022 DATLEE010000000
VERSION     DATLEE010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770399"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VM692_00490"
     CDS             complement(<1..839)
                     /locus_tag="VM692_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HUQ50663.1"
                     /translation="MESPFDIAADLDTPVSAYMKLKPFKPRFLLESVESGERLARYSF
                     IGFGDCMELRLDERGLTLDGRTSAAPRGQAELLQALRAALARAPQPSPSIPGVPLLGG
                     LVGVAGYDLVRNFERLPARAPVIEPTPQAHYLAPQSLLVFDHLTRGVALLHAGSESER
                     ISLRKQVIEALRGGIPHTNKRLRYSPAIGSLSEAEVTQGVHRTKEYIAAGDVYQLVLS
                     VRFAGQCEMEPFETYRALRLLNPSPYMYYCDLGDCVVVGSSPEALVKLTHGHAELRPI
                     AGTR"
     gene            1252..3042
                     /locus_tag="VM692_00495"
     CDS             1252..3042
                     /locus_tag="VM692_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ50664.1"
                     /translation="MSFWRYSRRALALVWSTHRGLTVGLAMLTLAAGVMPAGIAYLGK
                     LIVDAVVAALAANQAGAAPDHARVLTLVALEGALVAALAGVQRGMDFCQSLLRVLLSQ
                     RVHFLILDKALTLSLAQFEDSELYDKLNRARQEASMRPLSLVNRTFALAQNMVSLGSY
                     AGLLLSFSPWAVAILVAAGLPAFIGETRFSGERFRAFQWRSPERRMLAYLEIVLARED
                     HVKEVKLFELGGRLLARFREIFRRIYADEKRLTIRRDGWGFVLGLVSSGAFYGAYAWI
                     ALATIDGAISLGDMTMYLLLFKQGQSAVAAMLSAIGGMYEDNLYLSNLYDFLEQPAGG
                     AFGVATSGGDPQAGIEFDHVSFTYPGATQPALRDVTLRVRPGKSVALVGSNGSGKTTL
                     VKLLAGLYAPTEGTIRYQGRDLREWDPVALRQRIGVIFQDFNRYQLTVGENIGAGDVA
                     AFEDRGRWTEAAAKGQAAEFIGQLPSAYETQLGRWFNNGQELSGGQWQRIALARAFMR
                     TGAEILVLDEPTAAMDAETEAQIFEHFRALTRGRIAVLISHRFSTVRYADHIVVLDDG
                     GVVEQGTHEELIALAGRYAHLFNLQARAYR"
     gene            complement(3292..4080)
                     /locus_tag="VM692_00500"
     CDS             complement(3292..4080)
                     /locus_tag="VM692_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50665.1"
                     /translation="MNTLHRICFAAAAIAAWPLAADDAPVERRNFVSCPVVRDTQSVP
                     CWLAEYDGELYFLTLQTDVSSPVTPPWLGHRVLVEGTIARDRPRICGGVVLEPVVLSV
                     LPELDAACNTPVLPAEARYNLTFEPPRPPGPSAGRLAFAGDAPPAAPPEPPREFLLRY
                     DFDGLVVFRHAGQLTAILDAARARKSRAIAITGHRGSVLLSNGTTLTEQPAVARRRAE
                     QVATLLRGAGLTDVEYDIQWQDDPGVADGRDDASLRRVMVALTP"
     gene            complement(4077..5603)
                     /locus_tag="VM692_00505"
     CDS             complement(4077..5603)
                     /locus_tag="VM692_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50666.1"
                     /translation="MAGSVRRQAQCALALLWLGAHAAAGEWWAPGDGRPLPAEALYAN
                     PAGALGILNTAGAVETRNHPFFTALGTNGRACVTCHQPQDAMALSVATVRERWQATAG
                     QDPLFAAIDGRNCPNLPSGDAAAHSLLLTRGLVRVALPWPPRDSAGSPLPVEFTLEVV
                     RDPTGCNTDPVYGLTSAQPTVSVYRRPRPVANTKYLTHNNFGVGPFIAKSGLPAFRDP
                     ETGLPTSMNMMADARATTLKIQAADAARTHLELPQALTTDALQGIVGFESQIYAAQAR
                     DAAAGELTNGPPGFGPQNLAAAPAGVLGNNTSRFVFPLGLAWPAADGSGGGASAQDDG
                     RAAIKRGHDVFMFRTFFIKDSMHLNSVGLGNPVKRTCSTCHGMHMTGMDVANGWMDIG
                     TTNLPWAREAPLNPWTKETPQLPLFKVTCNADVAPHAFLGRVIYTQDLGRALISGKCN
                     DVGAIVMQQFRGLAARAPYFSNGSAATLRELVDFYDRRYDIGYSEQEKQDLVAFLQAL
                     "
     gene            5701..8436
                     /gene="ppc"
                     /locus_tag="VM692_00510"
     CDS             5701..8436
                     /gene="ppc"
                     /locus_tag="VM692_00510"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HUQ50667.1"
                     /translation="MSSSTSRTVRANIFFAEKDRALREDVHRLGELVGELVREQGGEA
                     LFDLVETARKLAIAHREGDQNAYGELKALLGALAPSTARDFIRAFSTYFQMVNMAEKV
                     HRIRRRRAYLRDASAPQPFGFVDILQRLKAQFVDGDEIERALAGIHIQPVFMAHPTEV
                     TRRTLLRKDHNIARHLVEMMDPYLTPHELEAMLGQIQQEMTTGWQTVESLDEGIRLRD
                     EAEHVLFFLTDVLYRVVPPLYESLESALAAAYPERGGGIRVPVIVQFGTWVGGDMDDN
                     PHVTAKSIRSTLARQRSLVLDLYYRECGELAAHLSQGSSRIGVSDELTRRTEQYAGHF
                     PEAAASLPVRHRQMPYRAYLRLVAARLKATYDDRAFPYESPSELVADLELMADSLRAN
                     KGRNAGLFAVRRLLRRVRTFGFFLATVDVRQNALVHRRVLGEALQERGWLAQDDEQRA
                     RRLEEALERRESPVGDLSSEGRRTLAVFQTIAHCRRKYGRDSIGPYIVSMAHGPDDVL
                     SVLLLAKWGHLGPKGEAVPLDIAPLFETGEDVERAPKIMERLLCDKLYREHLRARGDH
                     QIVMLGYSDSYRDGGIAAARWSLQKAQQALVATMERHGVALTVFHGRGGTISRSGGAL
                     HEAVLAAPRGAIAGRLRMTEQGENINTKYGLRGIALRTFEQLLSAVLLVTVRPPAENP
                     SEARWQSIMEEIAVASRGAYQSLLSDPDDFMEYFRAATPIDVIERIGSRSERAEHVSE
                     SVSEPRAAPWVFAWTQSRCLLPSWYGVATGLKKAIDLHGEDVLVEMFEQWHFFRVLLS
                     DVATALAKADLDVAARYSRLAGDRHEKFFPTIRREYKSCVDLVLRLTRQNELLEASRT
                     LRRAIRLRNPYVDPISFLQVDMLERWRADGRPDGPVLQALTASINGIAHAMQGTG"
     gene            complement(8433..>8759)
                     /locus_tag="VM692_00515"
     CDS             complement(8433..>8759)
                     /locus_tag="VM692_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50668.1"
                     /translation="CKLLDRERCRCRRYRQRQDLVADCVALRAGDARTFDWLPTTCAY
                     RKLAGGKPLEWWHPLVSGDPETVHRAGISVRGRSLAERDVAVEQLDTRVIRWIKTAPL
                     KRRAKR"
BASE COUNT         1396 a         2944 c         3011 g         1408 t
ORIGIN      
        1 cgcgtgccgg cgatcggtcg cagctccgca tgaccgtgcg tgagtttcac cagtgcttcg
       61 ggcgaggagc cgacgaccac gcagtcgccg agatcgcagt agtacatgta gggcgaagga
      121 ttcagcaacc gcagcgcgcg ataggtctcg aacggctcca tctcgcattg acccgcgaag
      181 cgcaccgaca gcactagctg atacacgtca ccggctgcga tgtattcctt ggtgcgatga
      241 acgccttgcg tgacttccgc ttcgctcagg ctgccgatcg ccggtgaata gcgcaggcgc
      301 ttgttcgtgt gcggaatgcc gccacgcaag gcttcgatga cttgcttgcg cagcgaaata
      361 cgttcgctct cgctgcccgc gtgcagcagc gccacaccac gcgtcaaatg atcgaatacc
      421 agcagcgatt gcggcgcgag gtagtgcgct tgcggagtcg gttcgatcac gggcgctcgt
      481 gccggcaagc gctcgaagtt cctgaccaga tcgtagccgg cgacgccgac cagtccgccg
      541 agcagcggca caccgggaat gctcgggctc ggctggggtg ccctcgccaa tgcagcgcgt
      601 aaggcctgaa gcagttcggc ctggccacgc ggcgcagcgc tagtgcggcc gtccagcgtc
      661 aggccgcgtt cgtcgagccg cagttccatg cagtcgccga aaccgatgaa cgaataacgt
      721 gcgagccgct cgccgctctc gacgctctcg agcaagaaac gcggcttgaa cggcttcagc
      781 ttcatgtacg ccgataccgg ggtatcgaga tcggcggcga tgtcgaaggg tgactccatg
      841 tgcgtttgcc ttgagtttag ggcccccaga aaacaaaaaa cccatctccg ttactcgcga
      901 agatgggttt gcaaaaagcc gaaagaggac ggaagtcttc tacgcgcgca ctacccgctt
      961 cgctggtgac gctcgccacc agcggccgtt gcaggcagga tgagtcggcc gtccgttcat
     1021 ggccgggcag tatctccggc tgcgtccccg cgggtcaagc cgcgatgagc ggccaagagc
     1081 ggcgctgtcg ttaagttgag tcgcgacggc ggagcaagga aggcgctctc aacgagtcga
     1141 aatttgcgca acacatgaat gagctcgcat accgataggc atagccccct gcgcccccca
     1201 cggaacatac gcgcagcggg tcaaggcccg aagacctcgc tcgacggcta tttgagcttc
     1261 tggcgctata gccgccgcgc gctcgcgctc gtgtggtcga cgcatcgcgg gttgaccgtc
     1321 gggctcgcga tgctgacgct cgccgccggc gtcatgcccg ccggaatcgc gtatctcggc
     1381 aagctgatcg tcgacgccgt cgtcgcggcg ctggccgcga atcaggccgg cgccgcgccc
     1441 gaccacgcgc gtgtgctcac gcttgtcgcc ctcgaaggag cgctcgtcgc ggcattggcc
     1501 ggcgtgcagc gcggcatgga tttctgtcag tcgctgctgc gcgtgctgct gtcgcagcgc
     1561 gtgcattttt taattctcga taaagcgctc acgctgtcgc tcgcgcaatt cgaggactcc
     1621 gagctctacg acaagctgaa tcgtgcgcgg caggaggcct cgatgcggcc gctgtcgctc
     1681 gtcaatcgca cgttcgcgct ggcgcagaac atggtatcgc tcggcagcta tgcgggcctg
     1741 ctgctctcct tctccccgtg ggccgtcgca attctcgtcg cggccggttt gcccgcgttc
     1801 atcggcgaga cgcgcttctc cggcgagcgc tttcgtgcgt tccagtggcg cagccccgag
     1861 cgccgcatgc tcgcgtatct agagatcgtg ctggcgcgcg aggaccacgt caaagaggtc
     1921 aagttgttcg agctcggagg ccggttgctc gcgcgcttcc gtgaaatctt ccgccgcatc
     1981 tatgccgacg aaaagcgcct gacgattcgg cgcgacggtt ggggtttcgt gctcgggctc
     2041 gtcagcagcg gagcgttcta cggcgcctat gcgtggatcg ccctcgccac gatcgacggt
     2101 gcaatctcgc tcggcgacat gacgatgtac ctgctgttgt tcaagcaggg gcagtcggcc
     2161 gtcgcggcga tgctgagcgc gatcggcggg atgtatgagg acaacctcta tctctcgaac
     2221 ctctacgatt tcttggagca gcccgcgggc ggtgccttcg gcgtcgcgac gagcggcggc
     2281 gacccgcagg ccggcatcga gttcgatcac gtgagcttca cgtaccccgg agcaacgcag
     2341 cccgcgctgc gcgacgtcac gttgcgcgtg cggcccggga agagcgtcgc gctcgtcggc
     2401 tcgaacggct cgggcaagac gacgctcgtg aagctgctcg cggggctcta cgcgccgacc
     2461 gagggcacca ttcgctatca aggccgcgat ttgcgcgagt gggaccccgt cgcgctgcgc
     2521 cagcgcatcg gcgtgatctt ccaggacttc aatcgctatc agctcacggt cggcgagaac
     2581 atcggcgccg gcgacgtcgc ggcgttcgag gatcgcggcc gctggacgga agcggccgcc
     2641 aagggccagg ccgccgagtt catcgggcaa ttgccgtcgg cttacgagac gcagctcggc
     2701 cgctggttca acaacggcca ggagctgtcg ggcggtcaat ggcaacgcat cgcgctggcg
     2761 cgcgcgttca tgcggacggg tgcggagatc ctcgtgctcg acgagccaac ggcggccatg
     2821 gacgcggaga ccgaggcgca gatcttcgag cacttccgtg cgctgacgcg cgggcgcatc
     2881 gcggtcctaa tctcgcatcg cttctcgacc gtccgttacg ccgaccacat cgtggtgctc
     2941 gacgatggcg gcgttgtcga gcaaggcact cacgaagagc tcatcgcgct cgccggccgc
     3001 tacgcgcacc tgttcaacct ccaagcgcgc gcgtaccgct gaccggcgtc gcgcgtgctg
     3061 gagggcgctg cgagcgggtg cttcgagggc gcggttgttc ctcccgcgct tgggccgccc
     3121 tgcaagcggg ccatgaattc acgatggtca ctccgaacgg ttaaagcgtt ccgcaacgac
     3181 gccccacttt gaatggcacg cccggcccgc gcgctgaaaa gcgcgatgcc aaacacaggc
     3241 ggcccgggcg gtagcgggag gaatgaccgc gccctcggag cacccgccca gctacggagt
     3301 gagtgcgacc ataacgcggc gcaacgacgc gtcgtctcgg ccatcggcaa cgcctggatc
     3361 gtcttgccat tggatgtcgt actcgacgtc ggtgaggccg gcgccgcgca gcagcgtggc
     3421 gacttgctcg gcgcggcgcc gcgccaccgc gggctgctcg gtcagcgtcg tcccgttcga
     3481 gagcagcacg ctgccgcgat gaccggtgat ggcgatcgcg cgcgacttgc gggcgcgcgc
     3541 ggcgtcgagg atggccgtca gctggccggc gtggcggaac acgacgaggc cgtcgaagtc
     3601 gtagcgcagc aggaactcgc gcggcggctc gggcggcgcc gcgggcggcg cgtcgccggc
     3661 gaaggcaagc cggccggcgc ttggccccgg cggccgtggc ggctcgaacg tcaggttgta
     3721 gcgcgcctcc gcgggcagca ccggcgtgtt gcacgccgcg tcgagctccg gcagcaccga
     3781 cagcacgacg ggctcgagca cgacgccgcc gcaaatgcgc ggccggtcgc gcgcgatcgt
     3841 gccctcgacg agcacgcgat ggccgagcca cggcggcgtc acgggcgagc tgacgtcggt
     3901 ctgcagcgtc aggaagtaca gctcgccgtc gtattcggcg agccagcacg gcaccgactg
     3961 cgtatcgcgc acgacggggc atgagacgaa gttgcgccgc tcgacgggcg cgtcgtcggc
     4021 cgcgagtggc catgcggcga tcgcagcggc ggcaaagcag attcggtgca gcgtgttcat
     4081 agcgcttgca agaacgcgac gagatcctgc ttctcctgct cggagtagcc gatgtcatag
     4141 cgccggtcgt agaagtcgac gagctcgcgc agcgtcgcgg ccgagccgtt cgagaagtac
     4201 ggggcgcgcg cggccaaccc gcggaattgt tgcatgacga tcgcgccgac gtcgttgcac
     4261 ttgcccgaga tcagcgcgcg gcctaagtcc tgcgtgtaga tcacgcggcc gagaaatgcg
     4321 tgcggcgcga cgtcggcatt gcacgtcacc ttgaacaacg gtagctgcgg cgtttccttg
     4381 gtccacggat tcagcggcgc ctcgcgcgcc cacggcaagt tcgtcgtgcc gatgtccatc
     4441 cagccgttcg cgacgtccat gcccgtcatg tgcatgccat ggcacgtcga gcacgtgcgc
     4501 ttgacggggt tgccgaggcc gacgctgttc aggtgcatcg agtccttgat gaaaaacgtg
     4561 cgaaacatga acacgtcgtg gccgcgcttg atcgcggcgc gaccgtcgtc ttgcgcgctt
     4621 gcgccgccgc cgctgccgtc cgcagcgggc cacgccagcc caagcggaaa cacgaaccgc
     4681 gacgtgttgt tgccgagcac gccggccggc gccgcggcga gattctgcgg gccgaacccc
     4741 ggcggcccgt tcgtgagctc gccggccgcg gcgtcgcgcg cttgtgccgc atagatctgc
     4801 gactcgaacc cgacgatacc ttgcagcgcg tcggtggtta atgcctgtgg cagctccagg
     4861 tgcgtgcgcg ccgcgtccgc cgcttggatc ttcagcgtcg tcgcgcgtgc gtcggccatc
     4921 atgttcatgc tcgtcggcaa cccggtctcg gggtcgcgga acgcgggcaa gccgctcttg
     4981 gcgatgaacg gtccgacgcc gaagttgttg tgcgtcaggt atttcgtgtt agcgacgggc
     5041 cgcgggcgcc gatagaccga gacggtcggc tgcgccgacg taagcccgta gacgggatcc
     5101 gtgttgcagc ccgtcggatc gcgcacgacc tcgagggtga actcgaccgg cagcggcgag
     5161 ccggctgaat cgcgcggcgg ccacggcaag gcgacgcgca cgagcccgcg cgtcagtagc
     5221 aacgaatgcg cggcagcgtc gccgctcggc agattcgggc agttgcggcc gtcgatggcg
     5281 gcgaacagcg ggtcctggcc ggccgtggcc tgccaacgct cgcgcaccgt ggccaccgac
     5341 agcgccatgg catcttgtgg ctggtggcac gtgacgcacg cgcggccgtt ggtgccgagc
     5401 gccgtgaaga acgggtggtt gcgcgtttcg accgcgcccg ccgtgttcag gatgccgagc
     5461 gcgcctgcgg gattggcgta cagagcctcg gccggtaacg gccggccgtc gccgggcgcc
     5521 caccactcgc ccgcggccgc gtgagcgccg agccagagca gcgcgagcgc gcattgcgcc
     5581 tgccgtcgca ccgagcccgc catctgccgc tccctccgca tccgtcgcgc gactatgccg
     5641 gcccgcaccg cgcgctgaca agccggcgcg tgcccgcgcg cggcgctatc atcggcggtc
     5701 atgtcctcat ccacgtcccg caccgtccgc gccaacatct ttttcgccga aaaggatcgt
     5761 gcgctgcgcg aggacgtgca tcggctcggc gagctcgtcg gcgagctcgt gcgcgagcaa
     5821 ggcggcgagg cgctgttcga cctcgtcgag acggcgcgca agctcgcgat cgcccatcgc
     5881 gaaggcgacc agaacgctta cggcgagctc aaggcgctgc tcggtgcgct ggcaccgagc
     5941 acggcacgcg acttcatccg cgcgttctcg acctatttcc agatggtcaa catggcggag
     6001 aaggtgcatc gcattcgccg ccgccgcgcg tatctgcgcg acgccagcgc gccgcaaccg
     6061 ttcggcttcg tcgacatcct gcagcggctc aaggcgcagt tcgtcgacgg cgacgagatc
     6121 gagcgcgcgc tcgcggggat ccacatccag cccgtgttca tggcgcaccc gaccgaggtc
     6181 acgcgccgca cgctgctgcg caaggatcac aacatcgctc gccatctcgt cgagatgatg
     6241 gacccgtacc tgacgccgca cgagctcgag gcgatgctcg gccagatcca acaggagatg
     6301 acgacgggct ggcagacggt cgagagcctc gacgaaggaa tccgcctgcg cgatgaagcc
     6361 gagcacgtac tgttcttcct gacggacgtg ctgtatcgag tcgtaccgcc gctctacgaa
     6421 agtctcgaga gcgcgctcgc ggcggcgtat cccgagcgcg gcggcgggat tcgcgtgccg
     6481 gtcatcgtgc agttcggcac gtgggtcggc ggcgacatgg acgacaatcc gcacgtcacg
     6541 gccaagagca ttcgctcgac gctcgcccgc cagcgctcgc tcgtgctgga cctgtactac
     6601 cgcgagtgcg gcgagctcgc cgcgcacttg agtcaaggct cgagccgcat cggcgtgtcc
     6661 gacgagctga cgcggcgcac ggagcaatac gccggtcact ttcccgaagc cgccgcgtcg
     6721 ctgcccgtgc ggcaccgcca gatgccgtat cgcgcgtacc tgcggctcgt cgcggcgcgg
     6781 ctcaaggcga cgtacgacga tcgtgccttt ccatacgagt cgccgagcga gctggtcgcc
     6841 gacctcgagc tgatggccga cagcttgcgc gccaacaaag gccgcaacgc cgggctgttc
     6901 gccgtgcgtc ggctgctgcg ccgcgtgcgg acgttcggct tctttctcgc cacggtcgac
     6961 gtgcggcaga acgcgctcgt gcatcggcgc gtgctcggcg aggcgctgca ggaacgcggt
     7021 tggctcgcgc aagacgacga gcaacgcgcg cgccggctcg aggaggcgct cgagcggcgc
     7081 gagtcgccgg tcggggattt gtcgtcggaa ggcaggcgta cgctcgccgt ctttcagacg
     7141 attgcgcatt gccggcgcaa gtacggccgc gactcgatcg gcccgtacat cgtcagcatg
     7201 gcgcacgggc ccgacgacgt gctgtccgta ttgttgcttg cgaagtgggg gcacctcggg
     7261 ccgaaaggtg aggccgtgcc gctcgacatc gcgccgctgt tcgaaaccgg cgaggacgtc
     7321 gagcgcgcgc cgaagatcat ggagcggctg ctctgcgaca agctctatcg cgagcacctg
     7381 cgcgcgcgcg gcgaccacca aatcgtcatg ctcggctatt ccgacagtta ccgtgacggc
     7441 ggcatcgcgg cggcgcgctg gagcctgcag aaagcccagc aggcgctcgt cgcgacgatg
     7501 gagcgccacg gcgtggcgct gacggtgttt cacggccggg gcggcacgat cagccgcagc
     7561 ggcggcgcat tgcacgaggc cgtgctcgcg gcgccgcgag gcgcgatcgc gggccgtctg
     7621 cgcatgaccg agcagggcga gaacatcaac acgaaatacg gcttgcgcgg catcgcgctg
     7681 cgaaccttcg agcagctctt gagcgccgtg ctgctcgtga cggtgcggcc gcccgcggag
     7741 aatccttccg aagcgcgttg gcaatcgatc atggaagaga tcgcggtggc gagccgcggc
     7801 gcttatcaga gcctgctctc ggaccccgac gacttcatgg agtacttccg cgcagcgacg
     7861 ccgatcgacg tcatcgagcg catcggcagc cgctccgagc gcgccgagca cgtcagcgag
     7921 tcggtcagcg agccgcgcgc ggccccgtgg gtgttcgcct ggacgcagag ccgctgcctg
     7981 ctgccgagct ggtacggcgt cgcgacgggg ctcaagaaag ccatcgacct gcacggcgag
     8041 gacgtcctcg tcgagatgtt cgagcagtgg cacttcttcc gcgtgctgct atccgacgtt
     8101 gcgacggcgc tcgcgaaggc cgatctcgac gtcgcggcgc gttactcgcg gctcgccggc
     8161 gatcgccacg agaagttctt tccaacgatc cgccgcgagt acaagagctg cgtcgatctc
     8221 gtgctgcggc tcacgcggca aaacgagctg ctcgaagcgt cgcgcacgct gcggcgtgcg
     8281 attcgcctgc gcaaccccta cgtcgacccg atcagcttct tgcaggtcga catgctggag
     8341 cgctggcgcg cagacgggcg gcccgacggc cccgtgctgc aggcgctcac ggcgagcatc
     8401 aacggcatcg cgcatgcgat gcagggaacg ggttagcgct tcgcgcgccg tttgagcggt
     8461 gccgtcttga tccagcgaat gacgcgcgtg tcgagctgct cgaccgccac gtcccgctcg
     8521 gcgagcgagc ggccgcgcac ggagatcccg gcgcgatgca cggtctcggg gtcgcccgag
     8581 acgagcgggt gccaccactc gagcggtttg ccgcccgcga gcttgcggta cgcgcacgtc
     8641 gtcggcagcc aatcgaacgt tcgggcatcg ccggcgcgta gcgcgacgca atccgcgacc
     8701 agatcctggc gctgccggta gcgccggcag cggcaacgct cgcgatcgag cagcttgca
//
LOCUS       DATLEE010000023         2625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2994233, whole genome shotgun sequence.
ACCESSION   DATLEE010000023 DATLEE010000000
VERSION     DATLEE010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2994233"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VM692_00520"
     CDS             <1..1139
                     /locus_tag="VM692_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HUQ50669.1"
                     /translation="YAAPAPLDPIVSAAMRDCLEREFGNPSSQHAAGRRARALVEAAR
                     AAVAARVCAPGERIVFTSGATESNNLALQGVLRRSRGRRVHLVTSRIEHKSVLDVAHW
                     LEASGVAVTQVPATPGGYVDPESVIEAMTADTQLVSIMHVNNETGVVQDVAAIGAACR
                     ARGVLFHVDAAQSVGKLPLALAAAPIDLCSLTAHKLCGPKGVGALYVAPRATLVPQLH
                     GGEQERGLRAGTLATHQIVGMGKAYELADAEGEAPRFTALRDRLWQELAAIPGVRQNG
                     DPSRRAAHVLNATFPGVDGESLRYELGDLAVSAGSACAADSPEASHVLAGMGLSDVLA
                     GSSLRFSVGRFTTVDEVVAAARSVAAAVERLRAVAKTAPAWCSA"
     gene            1169..1534
                     /locus_tag="VM692_00525"
     CDS             1169..1534
                     /locus_tag="VM692_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HUQ50670.1"
                     /translation="MEYSLAVQRHFAAPRRARELPAGAVGLVAGEAEDRTLHVWVRFQ
                     LQVVEDVVVATGFQAFGCPHTIAAASVVADWLEGRSVAAARQLDVKAVCAELEAPVEK
                     LGKLLRIEDAVVACWRIGA"
     gene            1551..1874
                     /gene="iscA"
                     /locus_tag="VM692_00530"
     CDS             1551..1874
                     /gene="iscA"
                     /locus_tag="VM692_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HUQ50671.1"
                     /translation="MSISLTDSAATRVRTFLEKRGHGVGLRLGVRRSGCNGWAYTIDY
                     ADSVAPDDVVFDQAGVKVIVAADNLELIDGTEVDFIKEGLNEAFKFRNPNVKGECGCG
                     ESFSV"
     gene            1927..2358
                     /gene="ndk"
                     /locus_tag="VM692_00535"
     CDS             1927..2358
                     /gene="ndk"
                     /locus_tag="VM692_00535"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012519000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HUQ50672.1"
                     /translation="MALERTFSIVKPDGVAKNLIGEVYRRFEKAGLKVVAAKMIRLRQ
                     DQAEGFYAVHKARPFYADLVRYMCSGPVMVQVLEGENAIKANRDIMGATNPKQAAPGT
                     IRADFAASIEENVVHGSDGPDTAKTEIAFFFSEAEICPRTR"
     gene            2340..>2625
                     /locus_tag="VM692_00540"
     CDS             2340..>2625
                     /locus_tag="VM692_00540"
                     /inference="COORDINATES: protein motif:HMM:NF044960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HUQ50673.1"
                     /translation="MPADALKLSIAGTPSKLSLLGMPAANLVEFFENLDEKPYRARQI
                     MRWLYQRYVTDYGEMTDLSAALRKQLGEMTTLALPPVLKHEHSDDGTRKWL"
BASE COUNT          435 a          840 c          915 g          435 t
ORIGIN      
        1 actatgcggc gccggcgccg ctcgacccaa tcgtgagcgc cgcgatgcgc gactgcttgg
       61 agcgcgagtt cggcaatcca tcgtcgcagc acgcggcggg ccggcgagcc cgcgcgctcg
      121 tcgaagccgc acgagccgcc gtcgctgcgc gcgtctgcgc gccgggcgag cgcatcgtct
      181 tcacgtcggg cgcgacggag tcgaacaatc tcgcgctgca aggtgtgttg cgccgctcac
      241 gcggccgtcg cgtgcatctc gtcacgtcgc gcatcgagca caagtcggtg ctcgacgtcg
      301 cgcattggct cgaggcaagc ggcgtcgccg tgacacaggt gcccgcaacg ccgggcggct
      361 acgtcgaccc cgagagcgtc atcgaggcca tgactgcgga tacgcagctt gtctcgatca
      421 tgcacgtcaa caacgagacc ggcgtcgtgc aggacgtcgc ggcgatcggt gccgcgtgcc
      481 gcgcgcgcgg cgtgctgttt cacgtcgacg ccgcgcagag cgtcggcaag ctgccgctgg
      541 cgctcgcagc ggcgccgatc gacctctgct cgttgacggc gcacaagctc tgcggaccga
      601 aaggcgtcgg cgcactgtac gtcgcgccgc gcgcgacgct cgtgccgcag ttgcacggcg
      661 gcgagcagga acgcgggttg cgcgccggca cgctggcgac gcaccagatc gtcggcatgg
      721 gcaaggccta cgagcttgcg gacgcggagg gcgaagcgcc gcgcttcacg gcgttgcgcg
      781 accggctgtg gcaagagctg gccgccatcc cgggcgtgcg tcagaacggc gacccgagcc
      841 gccgcgcggc ccatgtgttg aacgcgacgt ttccgggcgt ggacggcgag agcttgcgct
      901 acgagctcgg cgacctggcg gtttcggccg gctcggcttg cgcggccgat agccccgagg
      961 cctcgcacgt attggccggc atgggcttga gcgacgtgct ggccggcagc tcgcttcgct
     1021 tcagcgtcgg ccggttcacg accgtggacg aggtcgtcgc cgccgcccgc agcgtcgcgg
     1081 ccgcggtcga gaggctgcgc gccgtcgcaa aaaccgctcc cgcgtggtgc tcggcctgac
     1141 gtttttcggc taaactattg acccgcctat ggaatacagc ttagccgtcc agcggcattt
     1201 cgcagcgcct cgacgggcgc gggaactgcc ggccggtgcc gtgggtctcg tggcgggcga
     1261 agccgaggac cggacgctgc acgtctgggt acgtttccag ctgcaagtgg tcgaagacgt
     1321 cgtcgtggca acgggcttcc aggcgttcgg ctgcccgcac acaatcgcgg cggcgagcgt
     1381 cgtcgccgat tggctcgagg ggcgttcggt cgcggcggca cgccagctcg acgtgaaggc
     1441 tgtgtgcgcg gagctcgagg cgcccgtcga gaagctcggc aagttgctgc ggatcgagga
     1501 tgcggtggtc gcctgttggc gcatcggcgc ttgaggaagg ttgaaacgga atgtcgatct
     1561 cattgacgga tagtgccgcc acgcgcgtgc ggacgtttct cgagaaacgc ggccatggcg
     1621 tgggtcttcg tctcggcgtg cgccgcagcg gctgcaacgg ctgggcgtac acgatcgact
     1681 acgccgatag cgtggcaccg gacgacgtcg tcttcgatca agccggcgtg aaggtcatcg
     1741 tggccgccga caacttggag ctcatcgacg gcaccgaggt ggatttcatc aaggaaggcc
     1801 tcaacgaggc attcaagttc cgcaatccca acgtcaaggg cgaatgcggc tgcggcgaaa
     1861 gcttcagcgt ttagtcagag cgcgagcgcg ccaccggttt ctcaactgta gctctcaagg
     1921 tgaacaatgg ctctcgagcg tacgttttcc atcgtcaaac ccgatggtgt ggcgaagaat
     1981 ctgattggtg aggtctaccg ccggttcgaa aaggcggggc tcaaggtcgt cgcggcgaag
     2041 atgattcgtc tgcgtcagga ccaggccgaa ggtttttacg ccgtacacaa agcccgcccg
     2101 ttctacgcgg atctcgtgcg ctacatgtgc tcgggccccg tgatggtgca ggtgctcgaa
     2161 ggcgagaacg cgatcaaagc gaaccgcgac atcatgggcg cgacgaaccc taagcaagcg
     2221 gcgcccggga cgatccgcgc cgacttcgcc gcttcgatcg aggaaaacgt cgtgcacggc
     2281 tccgacggcc ccgacacggc caagaccgag atcgcgtttt tcttcagtga ggctgaaatt
     2341 tgcccgcgga cgcgctaaag ctgtcgatcg ccggcacgcc gagcaagctg tcgctgctcg
     2401 gcatgcccgc ggccaatctc gtcgagttct tcgagaatct cgacgagaag ccgtatcgcg
     2461 cgcggcagat catgcgctgg ctgtaccaac gctacgtgac tgactacggc gagatgacgg
     2521 atttgagcgc ggcgctgcgt aagcagctcg gcgagatgac gacgctcgcg ttgccgcccg
     2581 tgctcaagca cgagcattcc gacgacggca ctcgcaagtg gctgc
//
LOCUS       DATLEE010000024         4407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_26464, whole genome shotgun sequence.
ACCESSION   DATLEE010000024 DATLEE010000000
VERSION     DATLEE010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26464"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /gene="rdgC"
                     /locus_tag="VM692_00545"
     CDS             complement(<1..278)
                     /gene="rdgC"
                     /locus_tag="VM692_00545"
                     /inference="COORDINATES: protein motif:HMM:NF016281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HUQ50674.1"
                     /translation="MFRSVRFYSVSSLWPESEQALSEKLAAAAFKPCGSYTERSLGFE
                     PPTGAADGPLARRVAGAALLRLRNQTRVLPTAAVNEALEVRIAEYRER"
     gene            complement(362..1585)
                     /locus_tag="VM692_00550"
     CDS             complement(362..1585)
                     /locus_tag="VM692_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015269670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ50675.1"
                     /translation="MSSTARPARPFTIPRGVWALGFVSLFMDISSEMIHALLPVFLVS
                     TLGASVTAVGVLEGTAEAIASITKVFSGTASDWLGKRKLLTVLGYGLAAVTKPLFALA
                     PTAGWVFAARGLDRIGKGIRGAPRDALLAELTPAPIRGAAFGLRQALDTVGAFAGPLL
                     AIVLMAATANSFRTVFWLAVLPAIVSVIVLVLFVREPPSERAQRDVARPRLAVLLGTL
                     GARFWWLVLVAALFTLARFSEAFLILKVADDGLSVGYVPAVLVVMNVAYALSAYPAGW
                     LSDRVNRWAVFAVGAALLIIADIVLALGSSVAVAAIGIALWGLHMGFTQGLLAALVAD
                     SAGADKRGTAFGVFNLVTGLALLAASVLAGVLWDRGGPELTFLAGAALTAVAAVVAFV
                     LYGAGKIDGRKTATG"
     gene            1654..2259
                     /locus_tag="VM692_00555"
     CDS             1654..2259
                     /locus_tag="VM692_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUQ50676.1"
                     /translation="MADITFYTNPMSRGRIVHWLLDELGAPYETKVLDLEKGDHKKPE
                     YLKINPMGKVPAIVHRGVVVTEAAAICTYLADAYPQAKLAPALDDPQRGTYLRWIFFG
                     AGCVEPAIIDKMFHRPAVERKGALGYGSYEDTLNTLESALKPGPYLLGQRFSAADVYV
                     GAQLQWGLMVKALEPRPVFLQYVERIAERPALQKLLKGASS"
     gene            2256..3416
                     /locus_tag="VM692_00560"
     CDS             2256..3416
                     /locus_tag="VM692_00560"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUQ50677.1"
                     /translation="MSAVEQRTDVIERAKRVGALARKHAQRSEELGKLDEPVAEALHE
                     QGLLGMWVPRAVRGGLELDPVTSLQVIENVSYGDPSAGWVLMAASLAIGTGAAYLKDE
                     AVKELFGGPRFPVIAGQGTRPGVAQPTKGGFNLSGSWSFASGIKHGTHIHTLGLIEGT
                     HEARIFVLPVGKAKLIDNWDVLGLRATGSIDYTTDNVFVAEAYTHPATTEEPLRGGNL
                     YRIGIIGFAGLCHSGWACGIGRRVLDELIAAVAEKAGRPGTVADSASFQEGLANVEAV
                     VRSARAFVYETWNDAWATLSRGDKLSVRQNTLIRLALAHSTWSAHEAATFAYKAAGTA
                     ALRAGTIQHLFRDMHAGTQHTTSSPPVYASIGRELGGLAKGKKWLFLDLVDQ"
     gene            3413..4207
                     /locus_tag="VM692_00565"
     CDS             3413..4207
                     /locus_tag="VM692_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUQ50678.1"
                     /translation="MSCTRRGVLAGAAAVAALGATRHARAQRDAPRELARLVAANRIL
                     ANEGVVDAFGHVSVRDPRNAQRFWLSRSRSPALVELDDLMEFDLEGAAIDARGRTAYG
                     ERMIHAAVYAARSDVHSVVHHHAYGVLPFTITVEPLRPVVHTASVIGAVIPVWDIRTK
                     FGATDMLVRTIEQGRDLAATLAANTCALLRGHGAVVAGASIQRAVLTAIYLQVNANVL
                     LQALPLGTPEALTAEEIERSAEAQFSPLALDRSWEYFCQRAGVDPG"
BASE COUNT          761 a         1482 c         1517 g          647 t
ORIGIN      
        1 atgcgctcgc gatactcggc gatccgcacc tcgagcgctt cgttgactgc cgccgtcggc
       61 agcacgcgcg tttgattgcg caaacgcaag agagccgcgc ccgcgacgcg gcgtgcgagc
      121 gggccgtctg cggcgcccgt cggcggctcg aagcccaagc tgcgctcggt gtaggacccg
      181 cacggcttga acgccgcggc agcgagcttc tcggacagag cttgctcgga ctcgggccac
      241 aggctcgaca cggaatagaa acggacgctg cgaaacatcg gcggcttctt cgcaatcgtt
      301 gaaatgggag ggcgggcagt atcgcgattc gctgcgtcgc tgtcgatatc gacttcgctg
      361 ctcaacccgt tgccgttttt cggccgtcga tcttgccggc gccgtagagc acgaacgcaa
      421 cgacggcggc tacggccgtc aacgcggcgc cggcgaggaa cgtgagctcg ggcccgccgc
      481 ggtcccagag cacgccggcg agcacgctcg ccgcgagcaa cgcgagtccc gtgacgaggt
      541 tgaagacgcc gaacgcggtg ccgcgcttgt cggcacctgc gctgtcggcg acgagcgcgg
      601 cgagcaggcc ttgagtgaag cccatgtgca agccccagag cgcgatgccg atcgccgcca
      661 cggcgacaga agagccgagc gcgagcacga tgtcggcgat gatcagcaac gccgcgccga
      721 cggcgaacac tgcccaacga ttgacgcggt cggagagcca acccgcggga taagcggaca
      781 gcgcgtaggc gacgttcatg acgacgagca cggccggcac gtaaccgacg ctcaagccgt
      841 cgtcggcgac ctttaagatc aagaacgctt cgctgaaccg cgcgagcgtg aacaacgccg
      901 cgacgagcac gagccaccag aaacgcgcgc cgagcgtgcc aagcagcacc gcgagccgcg
      961 gccgcgcgac gtcgcgctgc gcgcgctcgc tcggcggctc gcgcacgaac aggaccagca
     1021 cgatcacgga cacgattgcg ggcagtaccg cgagccagaa caccgtccgg aacgagttgg
     1081 ccgttgccgc catcagcacg atcgcgagca gcggtcccgc aaatgcgccg accgtgtcga
     1141 gcgcctggcg caagccgaat gccgcgccgc ggatcggcgc cggcgtcagc tcggcgagca
     1201 gcgcgtcgcg cggtgcgccg cgaatgcctt tgccgatgcg gtcgaggccg cgtgccgcga
     1261 acacccaacc cgccgtcggc gcgagcgcga acaacggctt cgtgacggcc gcaagcccat
     1321 agccgagcac cgtcagcagc ttgcgcttcc cgagccagtc gctggccgtg ccggagaaca
     1381 ccttcgtgat cgacgcgatg gcttcggccg tgccttcgag cacgccgacg gccgtcacgc
     1441 tggcgccgag cgtgctgacg aggaacacgg gcagcagggc atggatcatc tcggacgaga
     1501 tatccatgaa caggctgacg aagccgagcg cccacactcc gcgcgggatc gtgaacgggc
     1561 gggccggacg cgcggtcgaa gacatggcgc gaggtttgca caggtgcagg cctgcggcta
     1621 gagtcgaacg cctaaaaacg acgggaaagg aacatggcgg acatcacgtt ctacacgaac
     1681 cccatgtcgc gcggccgaat cgtgcattgg ttgctcgacg agctcggtgc gccgtacgag
     1741 acgaaggtcc tcgacctcga gaagggcgac cacaagaagc ccgagtactt gaagatcaat
     1801 cccatgggca aggtgcctgc gatcgtccat cgcggcgtcg tggtcacgga agcggccgcg
     1861 atttgcactt atctcgccga tgcgtacccg caggcgaagc tcgcgccagc gctcgacgat
     1921 ccgcaacgcg gcacgtactt gcgttggatc ttcttcggcg ccggctgcgt cgagcccgcg
     1981 atcatcgaca agatgtttca ccggcccgcg gtcgagcgca aaggcgcgct cggctacggc
     2041 agctacgaag acacgttgaa cacgctcgag tctgcgctga agccggggcc gtacctgctc
     2101 ggccagcgtt tcagcgccgc cgacgtctac gtcggcgcgc agctgcagtg ggggctcatg
     2161 gtgaaggcgc tcgagccgcg tcccgtattt ctgcaatacg tcgaacgcat cgcggagcgg
     2221 cccgcgctgc aaaagttgtt gaaaggagcg tcgtcgtgag cgctgtcgag caaagaacgg
     2281 acgtcatcga gcgcgcgaag cgcgtcggcg ccttggcgcg caagcatgcg cagcgcagcg
     2341 aggagctcgg caagctcgac gagcccgtcg ccgaggcgct gcacgagcaa gggctgctcg
     2401 ggatgtgggt gccgcgcgcg gtgcgcggcg ggctcgagct cgatcccgtc acgtcgctcc
     2461 aagtcatcga gaacgtctcg tacggcgatc cgtccgcggg ctgggtgctg atggcggcgt
     2521 cgctcgcaat cggcaccggc gcggcgtacc tgaaagacga ggccgtcaaa gagttgttcg
     2581 gcggcccgcg cttccccgtc atcgcggggc agggcacgcg gcccggtgtc gcgcaaccga
     2641 cgaagggcgg cttcaacttg agcggctcgt ggagcttcgc gtcgggcatc aagcacggga
     2701 cgcacatcca cacgttgggc ttgatcgaag gcacgcacga agcgcgcatc ttcgtgctgc
     2761 cggtcggtaa ggcgaagctc atcgacaact gggacgtgct cgggctgcgc gcgacgggca
     2821 gcatcgacta cacgaccgac aacgtgttcg tcgccgaggc ttacacgcac cccgcgacga
     2881 cggaggagcc gctgcgcggc ggcaacctct atcgcatcgg catcatcggt tttgcgggcc
     2941 tttgccattc gggctgggcc tgcggcatcg gtcgccgcgt gctcgacgag ctgattgcag
     3001 ccgtcgccga aaaagcgggc cggcccggca cggttgcaga cagcgcgagc ttccaagagg
     3061 gtcttgcgaa cgtcgaagcc gtcgttcgct cggcgcgcgc gttcgtgtat gagacgtgga
     3121 acgacgcctg ggcgacgctg tcgcgcggcg acaagctgtc ggtgcgccag aacacgctga
     3181 ttcggctcgc gctcgcacac tcgacgtggt cggcgcacga agccgcgacg ttcgcgtaca
     3241 aggccgcggg caccgcggcg ctgcgcgccg gcacgatcca gcacctgttc cgcgacatgc
     3301 acgccggcac gcagcacacc acgtcctcgc cgcccgtcta cgccagcatc ggccgcgagc
     3361 tcggcggcct cgccaaaggc aagaagtggc tgttcctgga tctcgtcgat caatgagctg
     3421 tacgcgccgc ggcgtcctcg ccggcgcagc tgcggttgcg gctctcggcg cgacgcggca
     3481 cgcgcgagcg cagcgcgatg cgccacgcga gctcgcgcgg ctcgtcgccg cgaatcgcat
     3541 actggcgaac gagggcgtcg tcgatgcgtt cggccacgtc agcgtgcgcg atccccgcaa
     3601 cgcgcagcgc ttttggctgt cgcgctcgcg cagcccggcg ctcgtcgagc tcgacgatct
     3661 catggagttc gatctcgaag gcgcggccat cgacgcgcgc ggccgaacgg cgtacggcga
     3721 gcgcatgatt cacgcggccg tgtacgcggc gcgctccgac gtgcactccg tcgtgcacca
     3781 ccacgcgtac ggcgtgctgc cgttcacgat caccgtcgag ccgctgcggc ccgtcgtgca
     3841 cacggcttcc gtgatcggcg ctgtgatccc ggtctgggat atccgcacca agttcggcgc
     3901 gacggacatg ctcgtgcgca cgatcgagca gggccgcgac ctcgcggcga cgctcgcggc
     3961 gaacacgtgc gcgttgctgc gcgggcatgg tgccgttgtg gcgggtgcgt cgattcagcg
     4021 agccgtgttg acggccatct acctgcaagt caacgccaac gtgctgctgc aagcgttgcc
     4081 gcttggcacg cccgaggcgc tgacggccga ggagatcgag cgctcggccg aagcgcagtt
     4141 ctcgccgctg gcgctcgata gaagctggga gtacttctgt cagcgcgccg gcgtcgatcc
     4201 cggctagcgt cgcgcagtct tctcgcttca gcttccttca gacggccggc ttggccgcag
     4261 gcttagaatc cgccgaggcg gcggacttgg gccgcgcagt cgaacaacaa ccttggggga
     4321 accgatgaaa actttgaagc tcgctctgat tccgctcgct ttggcggcgc tggccggttc
     4381 ggcaggcgcg cagcagcagc aacagcc
//
LOCUS       DATLEE010000025         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_27474, whole genome shotgun sequence.
ACCESSION   DATLEE010000025 DATLEE010000000
VERSION     DATLEE010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27474"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            532..1359
                     /locus_tag="VM692_00570"
     CDS             532..1359
                     /locus_tag="VM692_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ50679.1"
                     /translation="MSPIKALLFATLAATSLARAALAANVDGANVHWTSTGNGPQAVI
                     FVHGWTCDESSWSGQVPAFSSKYRVITLDLPGHGKSAAPANGKFSMANFANAVEAVRA
                     KAGVDRVVVVGHSMGTPVVRTYQLMYPQHVAGLVIVDGAILLPGAPSGFTPPNVAGPE
                     GRAAREGMIRGMFSAATTPQLQDHILKMMLGAPESTAIGAMGAMFNDPSWQSAEPTKI
                     PVLGVFAANSQLDDSPAMKTVFPTAEFHEVPGTGHFLMLEKPAEFNKLLGDFLAKLH"
     gene            complement(1434..>2196)
                     /locus_tag="VM692_00575"
     CDS             complement(1434..>2196)
                     /locus_tag="VM692_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HUQ50680.1"
                     /translation="EMWGPWADNDAVHAALLEAGKKHGMVEVGGRAYHTNALESGWLP
                     RPLPAIYSGDKMKPYREWLTDNAYETTAPLGGSFYSKNIEDYYFTPYELDYGRIIGFD
                     HDFIGREALERMVAEGKTERRKKVTFVWNGDDTAAAYGSMFHKGPGAKFINLPMSLYD
                     TFHADRVEANGKVVGLSTWTGYSANERAILSLGTIDAAHAKPGTDVTLIWGEDKPSGK
                     AQVEDHVQFKIRAKVMPAPLVEQARTAYRSDAGLK"
BASE COUNT          397 a          718 c          685 g          396 t
ORIGIN      
        1 tcggcagcat gctcggcgtc ctcgatctgc ttgagcccca cggtgcgatc cgcgccgttg
       61 tcgtagcgcc gcagcacgtc cgcgagcgcc cgtgccccgt ccaccgtgag cttggcgtgg
      121 ttgtcgaata gaccgaagaa acgcccctcg cggggcatca gacgggcgat gaagttcatt
      181 gttgttcgca gccccctgaa cgcttcgagc gcggagtgta gtggccgcgg gcaagttgta
      241 acaaggccgt aatttatgcc ttgcggacgc gcggctcagg cacgacacga acccgtcgtt
      301 gggcaacaaa gccgccggcc actccgccgg cgatcttcaa gccgtgcgct acccaataaa
      361 gggtgggctc ccaaaaggta cggttgagcg cgaaccacaa cgtcatgaac agcaagatcg
      421 caacgacggc gaccagcact ccgcaggatg acatcgaaag cacggacgat agcggcacga
      481 tctcggctgt gtgagaatca tcgacgcttt gccccgacac ggagaattcc aatgagcccg
      541 atcaaagccc tgctcttcgc gacgctggcc gcaacatcgc tcgctcgcgc ggctttggct
      601 gcgaacgtcg acggtgccaa cgtgcattgg acatcgaccg gcaacgggcc gcaggccgtg
      661 atcttcgtgc acggttggac gtgcgacgag tcgtcgtgga gcgggcaggt gcccgccttc
      721 agctcgaagt accgcgtgat cacgctggac ctgccggggc acggcaagag tgccgcaccg
      781 gccaacggca agttcagcat ggcgaacttc gccaatgccg tcgaagccgt ccgcgccaag
      841 gccggcgtcg atcgcgtcgt cgtagtcggc cacagcatgg gtactccggt ggtccggacg
      901 tatcagctca tgtatccgca gcacgtcgcg ggcctcgtga tcgtggacgg cgctattttg
      961 ctccccggcg cgcctagcgg gttcacaccg ccgaacgtgg cgggtcccga gggccgcgct
     1021 gcacgcgaag gaatgatccg cggcatgttc agcgccgcga cgactccgca actccaagat
     1081 cacatcttga aaatgatgct cggcgccccg gaaagcaccg ccattggcgc gatgggcgcg
     1141 atgttcaacg atccgtcgtg gcagagcgcg gaacccacga agatcccggt gctcggcgtc
     1201 ttcgccgcga actcacagct cgacgacagc cccgcgatga agacggtatt cccgaccgcc
     1261 gagtttcacg aggtccccgg cacgggccac ttcttgatgc tcgagaaacc ggcggagttc
     1321 aacaagctgc tcggcgactt cctagcgaag ctccactgac gaaacgcatc gccgagccgc
     1381 gctcgccggg cgcgtccgta gcagcacctc gctgacatcg aagaagcggc ggcctacttc
     1441 aagcctgcat cggagcgata ggccgtgcgg gcttgctcga ccagcggcgc gggcatgact
     1501 ttcgctcgaa tcttgaactg cacgtggtcc tcgacctgcg cttttccgga tggtttgtct
     1561 tcaccccaaa tcagggtcac atccgtgccg ggttttgcgt gcgccgcgtc gatcgtgccg
     1621 agagacaata ttgcacgctc gttcgccgaa tagccggtcc aggtcgagag cccgacgacc
     1681 ttgccgttcg cctcgactcg gtcggcgtgg aacgtgtcgt agagcgacat cggcaggttg
     1741 atgaacttcg caccggggcc tttgtggaac atcgacccgt aggcggccgc ggtgtcgtcg
     1801 ccgttccaga cgaaagtcac cttcttgcgc cgctcagtct taccttccgc gaccattctc
     1861 tcgagcgctt cacggccgat gaagtcgtga tcgaagccga tgatgcggcc gtagtcgagc
     1921 tcgtacggcg tgaagtagta gtcctcgatg ttcttcgagt agaagctgcc gccgagcggc
     1981 gccgtcgtct cgtaggcatt gtcggtcagc cattcgcgat aaggcttcat cttgtcgccc
     2041 gagtagatcg caggcagcgg gcgcggcagc cacccggatt cgagcgcgtt cgtgtggtac
     2101 gcgcggccgc cgacctccac cattccgtgc ttcttgccgg cctcgagcag cgccgcatgg
     2161 acggcgtcgt tgtcggccca cggcccccac atctcg
//
LOCUS       DATLEE010000026         2469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_28263, whole genome shotgun sequence.
ACCESSION   DATLEE010000026 DATLEE010000000
VERSION     DATLEE010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28263"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2294)
                     /locus_tag="VM692_00580"
     CDS             complement(<1..2294)
                     /locus_tag="VM692_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HUQ50681.1"
                     /translation="MRAEVGGTRALIRALAGEIGKFRGRVLVATVFLILAKVAAVVVP
                     LVLKAIVDVMSRPEALAALPVLLLVGYAAVRFAATLFTELRDVTFARVTQNIVASLSL
                     RTFEHLHSLSARFHANRATGALTREVERGTAAVGFLTGAALFQILPTVVEIAAVLIIM
                     IANYGWLFSSLLLATFVLYAAHTSVFIRRRAIRQRRVNALESQAHRRLVDSLLNHETV
                     KSYANEAFETQQLGGILSRTVAAGVDNQMALTQLHVGQSAAIAMGIAAVMLMAGASIL
                     NGDLTVGDLVLVNAYVIQVCLPLNSLGFVIRESTDALVRAESLFGLLRLRPEARVRSG
                     APAATFARGPVRFENVRFGYEQSRQILADVSFTIEPGTTVAVVGGSGSGKSTLARLLL
                     KLFEPASGSISIGGVDIASIDARELRTNIGLVAQDTTLFNDTVEYNIAYGNVGASTEQ
                     IIAAAKAANVHEFVVELPERYATLVGERGLRLSGGERQRIAIARAILKDPSILVLDEA
                     TSALDSRAERAIRGTLERLAALRTTLVIAHRLSTVVDANEILVLEAGRIVERGRHVEL
                     LALDGLYARMWALQQQEHELQVRKRRARLEPVNLVALATDAIEAARPALDAKGLHLYT
                     TLGSEGSLVMGDHGALHEVVTDLVQHAVAVSEPGARVEVTLERSGNEIVLRISDTQRS
                     GVPGPQSAPPGQPPPAHRFDPSTLRAIVEEQHGRLAVEDVEPAGTMYTVALPVRAVAL
                     PKSDAAAPLAPAVEEPRFEGRSLLV"
BASE COUNT          400 a          855 c          840 g          374 t
ORIGIN      
        1 acgagcaatg accgcccttc gaagcgcggc tcctcgacgg cgggcgcgag cggcgccgcc
       61 gcatcggact tcggcaacgc gacggcgcgc accggcaacg cgaccgtgta catcgtgccc
      121 gcaggctcga cgtcttcgac ggcgagccgc ccatgctgct cctcgacgat cgcgcgcaac
      181 gtgctcggat cgaagcggtg agcgggcggc ggctggcccg gcggcgcgct ctgcgggccc
      241 ggcacgccgc tgcgctgcgt gtcgctgatg cgcagcacga tctcgttgcc cgagcgctcg
      301 agcgtcacct cgacgcgcgc gccgggctcg ctgacggcga cggcgtgctg gacgaggtcc
      361 gtcacgacct cgtgcaatgc gccgtggtcg cccatgacca gcgagccttc ggagccgagc
      421 gtcgtgtaga ggtgcaaccc cttggcatcg agcgccggcc gcgcggcctc gatggcgtcg
      481 gtggcgagcg cgaccaagtt cacgggctcg agccgggcgc gccgcttgcg aacctgcagc
      541 tcgtgctctt gttgctgcag agcccacatg cgcgcgtaga gcccgtcgag cgcgagcagc
      601 tccacgtgcc gcccgcgctc gacgatgcgg cccgcttcga gcacgagaat ctcgttcgca
      661 tcgacgaccg tcgagaggcg atgtgcgatg accagagtcg ttcgcagggc agccagccgc
      721 tccagcgtcc cgcgaatcgc gcgctcggcg cggctatcga gagccgacgt cgcctcgtcg
      781 agcacgagaa tcgaagggtc cttgagtatc gcgcgcgcaa tcgcaatgcg ctgccgctcg
      841 ccgcccgaca ggcggagccc gcgctcgccg acgagcgtcg catagcgctc ggggagctcg
      901 acgacgaact cgtgcacgtt ggccgccttc gcggcggcga tgatttgctc cgtgctcgcc
      961 ccgacgttgc cgtaggcaat gttgtattcg acggtgtcgt tgaacaacgt cgtgtcctgc
     1021 gccacgagac cgatgttcgt gcgcagctcg cgcgcgtcga tactcgcgat gtcgacgccg
     1081 ccgatgctga tcgagccgct cgccggctcg aacagcttca acagcaggcg cgccagcgtc
     1141 gatttgccgg agccgctgcc gccgacgacg gcgacagtcg tgccgggctc gatcgtgaag
     1201 ctgacgtccg cgagaatctg ccggctctgc tcgtagccga acctgacgtt ctcgaaccgc
     1261 acggggccgc gcgcgaacgt ggccgccggt gccccgctgc gcacgcgcgc ctcgggccgc
     1321 aggcgcagca gcccgaacag gctctcggca cgcacgaggg cgtccgtcga ctcgcgaatg
     1381 acgaagccca gcgagttcag cggcagacag acctggatca cgtaagcgtt gacgagcacg
     1441 aggtcgccga cggtcaagtc gccgttcagg atcgaggcgc ccgccatcag catgacggct
     1501 gcaatgccca tcgcgatcgc cgcactctga ccgacgtgca gctgcgtcag cgccatttga
     1561 ttgtcgacgc cggccgccac ggtgcggctc agtattccgc cgagctgctg tgtttcgaac
     1621 gcttcgttgg catagctctt gacggtctcg tgattcagca agctgtcgac gagccgccga
     1681 tgcgcctgcg actcgagcgc gttgactcgc cgctggcgga tcgcacggcg tcgaatgaac
     1741 acgctcgtgt gcgcggcata gagcacgaac gtcgcgagca gcaacgacga gaacagccaa
     1801 ccgtagtttg cgatcatgat gatcagcact gcggcgatct ctacgacggt cggcaaaatc
     1861 tggaacaacg cggcacccgt caggaaaccg acggcggccg tgccgcgctc gacttcacgc
     1921 gtcagcgcac cggtcgcgcg attcgcgtgg aagcgcgcgc tcagcgagtg caagtgctcg
     1981 aacgtccgca ggctcaagct tgcgacgatg ttctgcgtca cgcgggcaaa cgtgacgtcg
     2041 cgcagctccg tgaacagcgt agccgcgaag cgcacggccg cgtagccgac gagcagcagc
     2101 acgggcagcg ctgcgagcgc ttcggggcgg ctcatgacgt cgacgatggc tttcaggacg
     2161 agcggcacga cgacggccgc gaccttggcc aagatcagaa acacggtggc cacgagcacg
     2221 cggccgcgga atttgccgat ctcgccggcg agggcgcgga tcagcgcgcg tgtgccacca
     2281 acctccgccc gcatctcgat ctctcagccg gttcgccgcg tctgcgctcc ggcggcgccc
     2341 gagcgtgggt gctgcagcag gtcgtcgaag tactcgatgg tccgcttgag cccgtcctcg
     2401 agggtcacct tcggttgcca gtcgagctcg gccgccgcaa gcccgatgtc gggccgacgc
     2461 cggacggga
//
LOCUS       DATLEE010000027         5363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8971105, whole genome shotgun sequence.
ACCESSION   DATLEE010000027 DATLEE010000000
VERSION     DATLEE010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8971105"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="VM692_00585"
     CDS             complement(<1..1691)
                     /locus_tag="VM692_00585"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HUQ50682.1"
                     /translation="MDGASNKTITAAEGLLRALKRRGIDYVLANAGTDFAPIIEGLVR
                     AAGRKDAIPRFITVPHENLAMAMAHGYYQVAGKPAGVMVHVTVGTGNTICALMNAARD
                     NVPLLLMAGRTPLTQDGDIASRSAPIHWGQENFDQGGITREYTKWDFELRAGQPVDEI
                     VGRALDVALSEPRGPVYLTLPREVLASPHKDSKTLLPATELAAPQPSARHIDTLAGWI
                     AAAEFPLILTSNLGRDTAAVGVLAELAERHAIPVAQPHASCINLPASHPFNLGHAGTE
                     LLGKADLLLVVDCEVPWYPRYAAPRADAKVVHLGVDPLYERYPHRAFTAQLAITGSSR
                     VGLELLDEALKARSTQRAAADRRRTIVAEFKKTKSAQAAAANERARNEKPIRYSYVGQ
                     CVHDALPKNGIVITELGVAVDAFALEEPGSLLAVSIGGGLGFGLGASLGAKLAAPDRM
                     VVSTIGDGSYMFGNPTPFHLVSRAAKLPTLTIVCNNGRWQAVESATRVVYPDGAAAHA
                     DEMPLVELQPSPEFTKVAEASDAWAKRVDDPAELPRAIDAALNAVAGGRQALLDVR"
     gene            complement(1757..2509)
                     /locus_tag="VM692_00590"
     CDS             complement(1757..2509)
                     /locus_tag="VM692_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HUQ50683.1"
                     /translation="MRRLVLKLCVAIALASTALVAAGDDLNGAPSWPTEEFHFVRMYY
                     DGAGRWGRGGWTTDYPEAEYHFTMGVKRLSRVDIGDQARMLDVNDDIIFDYPWLYAVE
                     VGSWYLDDTSAARLREYLLRGGFLMTDDFHGSQEWQGFVESMQRVFPDRPIVEIEDSD
                     EVFHVLYDTDQRIQIPGIAALRYGVTYERDGVVPHWRGIYDDDGRLMVAINFNMDLGD
                     AWEHADDPQYPEAMTGLAYRFAVNYVVYAMTH"
     gene            complement(2542..2766)
                     /locus_tag="VM692_00595"
     CDS             complement(2542..2766)
                     /locus_tag="VM692_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50684.1"
                     /translation="MDSVAKKAVVAEFLTRCNRYAEGQLEKYRTQLDSASPGEALAVQ
                     DKIGHWTAYRAFNEHALQELARGELDDWFT"
     gene            2988..5231
                     /locus_tag="VM692_00600"
     CDS             2988..5231
                     /locus_tag="VM692_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50685.1"
                     /translation="MFEFLFKYSRATYERAELVFASGWPVWLLYASIVAAAIAIGIVI
                     WRRKAGLGIVRATVLGALQTALVAALLVLAWRPALVTQTLRPQENSVAVLLDTSGSML
                     YGDKDTSRLQQAVDSLSERALPALESKFAVNLFSFSGDLVELPSLEQVPPPGPVTHVG
                     DALLNVLRGAQSGAIAAVVLVSDGADNSADFDAAKIAEIASFGVPVHTVGVGAETMAN
                     DLELEDVQLPSIGLPGSTVSAQVSIRHSGATLAQLKVYDGDAILASEPIQLPSTSGVT
                     TRWVDIAVGEAGVRDLRFALDALPGETNVINNSRLRPMEVPEQRRHILYIEGEPRWEY
                     KFIRRAIDENPAVRVASLLKTTPNKFYRQGVESADELTAGFPTEELELFRYDALMIGS
                     FEAAALTPAQQDMIREFVSRRGGSLLMLGGRRGLTDGGWGATSVAEVLPVRLPEIDGP
                     SFMREPAKAVLAADGRTSALTRLAPTDEENEATWATLPDLADFQTLGPAKPGAQTLLN
                     AEFHGRTEPLLVEQRYGNGHAYVLATGGTWRWQMQLPHEDQKHETFWRQLLQAAATTA
                     PQPVTLTSDRVFYGDESTVLLRAEVRDKTYKPSTDAAVTLEVSDGMGPPTRVEMTPVA
                     GERGLYEAAYETAHTGVFRFEAVAKTDAEELGRARFAVRREDGVIEHYRVAQNRPLLE
                     RLSAATGGSYFGVDDVSKLPEAVSFSEAGSVERQVLDLWNIPIAFLLLILLKAGEWLL
                     RLYWGRL"
BASE COUNT          837 a         1763 c         1866 g          897 t
ORIGIN      
        1 cgcacgtcga gcagcgcttg ccggccgccg gctactgcgt tcagcgcggc gtcgatcgcg
       61 cgcggcagct cggccggatc gtcgacgcgc tttgcccacg cgtccgacgc ttcggcaacc
      121 ttcgtgaact cgggcgacgg ctgcagctcg acgagcggca tctcgtcggc atgcgccgcg
      181 gcaccatcgg gatagacgac gcgcgtcgcg gattcgacgg cctgccagcg gccgttgttg
      241 cagacgatcg tcagcgtcgg cagcttcgcg gcgcgcgaga cgaggtggaa cggcgtcggg
      301 ttgccgaaca tgtagctgcc gtcgccgatc gtgctgacga ccatgcgatc cggcgccgcg
      361 agcttcgcgc cgagcgaagc gccgaggccg aagcccaagc cgccgccgat gcttacggcg
      421 agcagcgagc ccggctcctc gagcgcgaag gcgtcgaccg cgacgccgag ctccgtgatg
      481 acgatgccgt ttttcggcag cgcgtcgtgc acgcattggc cgacgtacga gtagcgaatc
      541 ggtttttcgt tgcgggcgcg ttcgttggcg gctgcggcct gcgccgattt cgtctttttg
      601 aactccgcga cgatcgtgcg gcgacggtcg gccgcggcac gttgcgtcga gcgcgccttc
      661 aacgcctcgt cgagcaactc gaggccgact cgcgagctgc ccgtgatcgc gagctgcgcc
      721 gtaaacgcac ggtgcggata gcgttcgtac agcgggtcga cgcccaggtg cacgaccttc
      781 gcgtcggcgc gcggtgccgc atagcgcgga taccacggca cttcgcaatc gacgacgagc
      841 agcaagtcgg ccttgccgag cagctcggtg ccggcgtggc ccaggttgaa cggatggctc
      901 gcaggcaagt tgatgcagct cgcgtgcggt tgcgcgacgg gaatcgcatg gcgctcggcg
      961 agctccgcaa gcacgccgac cgctgcggtg tcgcggccga gattcgaggt caggatcaac
     1021 ggaaactccg ccgcggcaat ccagccggcg agcgtgtcga tatggcgcgc gctcggctgc
     1081 ggcgccgcga gctcggtcgc cggcagaagc gtcttgctgt ccttgtgcgg gctcgcgagg
     1141 acctcgcgcg gcaacgtgag atacacgggg ccgcgcggct ccgacaaagc gacgtcgagc
     1201 gcccggccga cgatctcgtc gacgggctgg ccggcgcgca gctcgaaatc ccacttcgtg
     1261 tactcgcgcg tgatgccgcc ctgatcgaag ttttcttggc cccagtgaat aggtgcggag
     1321 cgcgacgcga tgtcgccgtc ctgcgtcagc ggcgtgcgcc cggccatcag cagcagcggc
     1381 acgttgtcgc gcgcggcatt catcagcgcg caaatcgtgt tgcccgtgcc gaccgtgacg
     1441 tgaaccatga cgccggccgg cttgccggcg acctggtagt agccgtgcgc catcgccatc
     1501 gcgagattct cgtgcggcac ggtgatgaag cgcggaatcg cgtccttgcg ccccgcggcg
     1561 cgcacgagcc cttcgatgat cggcgcgaag tcggtacccg cgttcgcgag gacgtagtcg
     1621 atcccgcggc gcttcagcgc tcgcagcagg ccttcggccg ccgtgatcgt tttattgctc
     1681 gctccatcca tagccggctc ctcgaggatg gccgcagttt agcggaactg ggcggtcccc
     1741 gagggggcgc acgcgctcag tgcgtcatcg cgtagacgac gtagttgacg gcgaagcggt
     1801 aggcgaggcc ggtcatggcc tccggatact gcggatcgtc ggcgtgctcc caagcgtcgc
     1861 cgagatccat gttgaagttg attgccacca tgaggcggcc gtcgtcgtcg taaatgccgc
     1921 gccagtgcgg cacgacgccg tcgcgctcgt acgtcacgcc gtagcgcaac gccgcgatgc
     1981 cggggatctg gatgcgctgg tccgtgtcgt acagcacgtg gaacacctcg tccgagtcct
     2041 cgatctcgac gatcggccga tccgggaaca cgcgctgcat ggactcgacg aagccctgcc
     2101 attcctgcga gccgtggaag tcgtccgtca tcaagaaacc gccgcgcagc aggtactcgc
     2161 gcaggcgcgc cgcggaggtg tcgtccaagt accagctccc gacctcgacg gcgtacagcc
     2221 acggatagtc gaagatgatg tcgtcgttga cgtcgagcat tctggcttga tcgccgatgt
     2281 cgacgcgcga caggcgcttg acgcccatcg tgaagtgata ctcggcttcg ggataatcgg
     2341 tcgtccaccc gccgcgcccc cacctgccgg cgccgtcgta atacatgcgc acgaagtgga
     2401 actcttcggt cggccagctc ggcgcgccgt tcaaatcgtc gccggccgca acgagcgcgg
     2461 tcgaggcgag cgcgatcgcg acgcagagtt tcagcacgag tcgtctcatc gagccaagac
     2521 tacggtgaag ccgtacggcc gtcacgtgaa ccagtcgtcg agctcgccgc gcgcaagctc
     2581 ttgcagcgcg tgctcgttga acgcgcgata ggcggtccag tggccgatct tgtcttggac
     2641 cgcgagcgcc tcgccgggcg aggcgctgtc gagctgcgtg cggtacttct cgagctgccc
     2701 ttcggcgtag cggttgcagc gcgtcaggaa ctcggcgacg actgcctttt tcgcgacgga
     2761 atccatcgaa gcgaagttcc tgccaggggc aaagctcgac cggatggtag cgtgcggcgc
     2821 gagtaccccg aaagcgcgcc gcattgggtc tagtcgcgag cggagaccgc acggtaagct
     2881 ccgactggcc ctgctgcttc gagcctcgcg caaccggtcg cttccggttt ctggagtttt
     2941 gttcagcgcg agcggagtgc ccggggcctc agcgtatggt gggccgaatg ttcgagttcc
     3001 tgttcaaata ctcccgcgcc acctatgaac gtgccgagct cgtcttcgcg agcggttggc
     3061 ccgtttggtt gttgtacgcc tcgatcgtgg cggctgccat cgcgatcggc atcgtcatct
     3121 ggcgccgcaa agccgggctc ggcatcgtgc gcgcgacggt gctgggcgcg ctgcagaccg
     3181 cgttggtcgc ggcgctgctc gtgctggcct ggcgaccggc gctcgtgacg cagacgctgc
     3241 ggccgcaaga aaactccgtt gcggtgttgc tcgacacgtc gggcagcatg ctctatggcg
     3301 acaaggacac gtcgcggctt cagcaagccg tcgacagtct cagcgagcgg gcgctgccgg
     3361 ctctcgagag caagttcgcc gtcaatctgt tctcgttctc gggcgacctc gtcgagctgc
     3421 cttcgctgga gcaagtgccg ccgccggggc ctgtcacgca cgtcggcgac gcgttgttga
     3481 acgtgctgcg cggcgcgcag tcgggagcca tcgccgccgt cgtgctcgtc tccgacggcg
     3541 ccgacaactc cgccgatttc gacgcggcca agatcgccga gatcgcgagc ttcggcgtac
     3601 cggtccacac ggtcggcgtc ggcgccgaga cgatggcgaa cgacctcgag ctcgaggacg
     3661 tgcagctgcc gagcatcggg ctgccggggt cgaccgtcag cgctcaagtc agcattcgcc
     3721 acagcggtgc gacgctggcg cagctcaagg tctacgacgg ggatgcgatt ctcgcgtccg
     3781 agccgattca gctgccgtcg acgtcgggcg tcacgacgcg ctgggtcgac atcgcggtcg
     3841 gcgaggccgg cgttcgcgat ctgcgcttcg cgctcgatgc gctgcccggc gagaccaacg
     3901 tcatcaacaa ctcgcgcctg cggccgatgg aagtgccgga gcagcggcga cacatcttat
     3961 atatcgaggg cgagccgcgc tgggagtaca agttcatccg ccgcgccatc gacgaaaatc
     4021 cggccgtgcg cgtcgcgagc ttgctgaaga cgacgccgaa caagttctat cgtcagggtg
     4081 tcgagtcggc cgacgagctg accgcgggtt ttccgaccga agagctggag ctgttccgct
     4141 acgacgcgct gatgatcggc agcttcgagg cggccgccct cacccccgcg cagcaggaca
     4201 tgatccgcga gttcgtgagc cggcgcggcg gcagcttgct gatgctcggc ggccggcgcg
     4261 gtctgaccga cggcggctgg ggcgcgacga gcgtcgctga ggttctgccc gtgcgcttgc
     4321 cggagatcga cggcccgagc ttcatgcgtg agccggcgaa ggccgttttg gccgcggacg
     4381 gtcggacgtc ggcgttgacg cggctcgcgc cgacggacga agagaacgaa gcgacgtggg
     4441 ccacgctgcc ggacctcgcc gattttcaga ccctagggcc ggcgaagccc ggcgcgcaga
     4501 ccttgctgaa cgcggaattc catggccgca cggagccatt gctcgtcgag cagcgctacg
     4561 gtaacggtca cgcctacgtg ctggcgacgg gcggcacttg gcgttggcag atgcagctgc
     4621 cgcacgagga ccagaagcac gagacgtttt ggcgccagct gctgcaggcc gcggcgacga
     4681 cggcaccgca gcccgtcacg ttgacgtcgg accgcgtgtt ctacggcgac gagagcaccg
     4741 tgctgctgcg cgccgaggtc cgcgacaaga cctacaagcc ttcgaccgat gccgccgtga
     4801 ccttggaggt cagcgacggc atgggtccgc cgacgagggt cgagatgacg cccgtcgccg
     4861 gcgagcgcgg cctgtacgag gccgcgtacg agacggcgca caccggcgtc ttccgcttcg
     4921 aggcggtcgc caagaccgat gcggaggaac tcggccgcgc gcgcttcgcc gtgcggcgcg
     4981 aagacggcgt catcgagcat taccgcgtcg cgcagaatcg gccgctgctc gagcggctat
     5041 ccgcggcgac gggcggcagc tatttcggcg tcgacgacgt cagcaaactg cccgaggccg
     5101 tgagcttttc ggaagccggc agtgtcgaac gacaagtgct cgatctctgg aacatcccca
     5161 ttgccttcct gctgctgatc ctgttgaagg ccggcgaatg gctgttgcgc ctgtactggg
     5221 ggcggctgtg agcgtgcgcg cgacggcagc gctgttgacc gccgcgctag gcgtcgcggc
     5281 gcccgcgcac gcggagctct attacttgat cgtcggcggc ctcggcggcg agccgggcta
     5341 cgaggagcaa ttcaagaagg atg
//
LOCUS       DATLEE010000028         3340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1522233, whole genome shotgun sequence.
ACCESSION   DATLEE010000028 DATLEE010000000
VERSION     DATLEE010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522233"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VM692_00605"
     CDS             <1..410
                     /locus_tag="VM692_00605"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HUQ50686.1"
                     /translation="ADRDDSGAIVSSGSVNAFQMRVGDCFDDGSTFTDDEVDHVPGVP
                     CAQPHDNEVYAVFDVGMTSFPGEQMGDLAQQACIERFEAFVGKDYESSSLDVASLYPT
                     SESWHGQNDREVICAVYDLEQKKLAGTVKGLRL"
     gene            543..2039
                     /locus_tag="VM692_00610"
     CDS             543..2039
                     /locus_tag="VM692_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50687.1"
                     /translation="MHPTHPTLRNGLSAAVAALCCATAYGQINDDIRGAPSEANHFIE
                     TPRGWQHPKTAWGEPDLQAQLNMMQAAGIPLERCANSYRAALPPGVAPAPGPGGGGGP
                     SVPCDMNKVWLTAEEYAARMEEFRSRVDASRQFAEQGNLGRAMLTGLTDPNIPQRQTT
                     LIMSPANGLLPALTPEGKRRAALAKSDWALPGETIAFDGPNDFDSWDRCITRGMPSSM
                     MPYRYNGGFAIRQAPGYVIFELEMIHEARVIPTTPRPPLDPEVKQWLGESRGHWEGNT
                     LVVETTNYHEGPPLINLAVVGSPPGNRFPHSDQMKTTERVVRLNDDTWLYEITTEDPV
                     ILTAPFTVRYPMRNDPAYFTPEYACHEDNTIVQNYTNTSRHERANPTPEPPQKPVQVA
                     PGVADALAGRWVGRPAIKTIDVDIELEFTKNADGTINGKLIGTNLGRIERTLRDFTVA
                     DNTVFFTLPNVDPWRFSGELGDGTVTGVIASAQGGVPVTFRKQTNRNR"
     gene            complement(2048..2395)
                     /locus_tag="VM692_00615"
     CDS             complement(2048..2395)
                     /locus_tag="VM692_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50688.1"
                     /translation="MAARRKAIDLQVSTHETKSPLEAEALAAVYAYEALLTRRNGKKT
                     RATGTWQAIRRYGAIEAVQQAVCRPPDQGTVTLRDLGLEDLAFEALVLRHEASFSAAA
                     IEASKARLAATGS"
     gene            complement(2526..2951)
                     /locus_tag="VM692_00620"
     CDS             complement(2526..2951)
                     /locus_tag="VM692_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50689.1"
                     /translation="MLDREIEKIIRRAGYDVRSDGLPGGTAAVYALRDGKRHTRSFPT
                     LLALAEHLGCLERPVVFIEDQETVATVMATHAKERNAFVVKPAACRVARFGRNKYVQL
                     VSAAGYASAMYEVRSDGLRRTSNGNLPPEVVAAFARAIS"
     gene            complement(2955..3158)
                     /locus_tag="VM692_00625"
     CDS             complement(2955..3158)
                     /locus_tag="VM692_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50690.1"
                     /translation="MVVTPEQLGMRSVGDCRNRAEQLGLTALPVLPYKQEFWRLDTLL
                     LWSRGNGFEYLPMLGLGLAPRLK"
BASE COUNT          614 a         1092 c         1074 g          560 t
ORIGIN      
        1 aggcggatcg tgacgatagc ggcgcgatcg tttcctcggg cagcgtgaac gcgtttcaaa
       61 tgcgcgtcgg cgactgcttc gacgacggct cgacgttcac cgacgatgag gtcgaccatg
      121 tgccgggcgt tccgtgcgct cagcctcacg acaacgaggt atacgccgtg ttcgacgtcg
      181 gcatgaccag ctttcctggc gagcaaatgg gtgacctcgc ccaacaggcg tgcatcgaac
      241 gtttcgaagc gttcgtcggc aaggactatg agtcgtcgtc gctcgacgtc gcctcgttgt
      301 atccgacgag cgaaagctgg cacgggcaaa acgaccgtga ggtcatttgc gccgtctacg
      361 acctcgagca gaagaagctc gcgggcaccg tgaagggctt gcgcctctag cgtgcgctcg
      421 cgcgcatccc gcattccccg ccgttcacac cgcgcgcgcg gcgcgtccgt aattggacgc
      481 tgatgccgcg cgcgggttaa tttaccaact ctaagatcag ctgaatgtgg cgggggaatg
      541 ccatgcatcc gacccaccca acgttgcgca atgggctgtc cgctgccgtc gcagcgctct
      601 gctgcgcgac ggcctacggc cagatcaacg acgacatccg cggcgccccg agcgaggcca
      661 atcatttcat cgagactccg cgcggctggc agcatccgaa gacggcctgg ggcgagcccg
      721 accttcaggc gcagctcaac atgatgcagg ccgcgggcat tccgctcgag cggtgcgcga
      781 atagctatcg ggcggcattg ccgcccggtg tggcgccggc tccggggccc ggcggtggcg
      841 gtggaccgtc cgtgccgtgc gacatgaaca aggtgtggct cacggccgaa gagtacgcgg
      901 cgcgcatgga ggaattccgc agccgtgtcg acgcgagtcg gcagttcgcg gagcagggca
      961 acttaggccg cgcgatgctg accggtctga cggaccccaa catcccgcag cgccagacga
     1021 cgttgatcat gagcccggcc aatggtttat tgccggccct gacgcccgaa ggcaaacgcc
     1081 gcgcggcgct cgcgaaaagc gattgggctt tgcccggtga gacgatcgcc ttcgacgggc
     1141 cgaacgactt cgacagctgg gaccgctgca tcacgcgcgg catgccgtcg tcgatgatgc
     1201 cgtaccgcta caacggcggc ttcgcgatcc gccaagcgcc gggctacgtg atcttcgagc
     1261 tggaaatgat tcacgaagcg cgcgtcatcc cgacgacccc gcggccgccg ctcgaccccg
     1321 aggtcaagca gtggctgggc gagtcgcgcg gccactggga aggcaacacg ctggtcgtcg
     1381 agacgacgaa ctatcacgag ggcccgccgc tgatcaatct ggcggtcgtc ggctcaccgc
     1441 cgggcaaccg gtttccacac agcgaccaaa tgaagacgac ggagcgcgtc gtgcggctca
     1501 acgacgacac gtggctctac gagattacga cggaagaccc cgtgattctg acggcaccgt
     1561 tcaccgttcg ttacccgatg cgcaacgacc cggcgtattt tacgccggag tacgcgtgcc
     1621 acgaggacaa cacgatcgtc caaaactaca cgaacacgag ccgacacgag cgcgcgaacc
     1681 cgacgccgga gccgccgcag aaacccgtgc aagtcgcgcc cggtgtcgcc gacgcgttgg
     1741 caggccgctg ggtcggtcgg ccggcgatca agacgatcga cgtcgatatc gagctcgagt
     1801 tcacgaagaa cgcggacggc accatcaacg gcaagttgat cggcacaaat ctaggccgca
     1861 tcgagcgaac gctgcgcgac ttcacggtcg cggacaacac ggttttcttc acgctgccga
     1921 acgtcgatcc gtggcgcttc tccggcgagc tcggcgacgg cacggtcacg ggcgtcatcg
     1981 cgagcgcgca gggcggcgtg cccgtgacgt tccgcaaaca gacgaatcga aatcgctaaa
     2041 gcgagctcta gcttccggtc gcggccaggc gtgccttcga agcctcgatc gctgcagcgc
     2101 tgaaagacgc ctcgtggcgc agaacgagcg cctcgaacgc gaggtcttcc agcccgagat
     2161 cccgcaatgt gacggttccc tgatcgggcg gccggcacac ggcttgctgc acggcttcga
     2221 tggcgccgta acgtctgatc gcctgccagg ttcccgtggc gcgcgtcttc ttaccgttcc
     2281 ggcgagtcag cagcgcttca tacgcgtaga ccgcggcgag agcttcggcc tccaagggcg
     2341 atttggtctc gtgcgtcgac acctgtaggt cgatcgcttt ccgtcgcgcc gccaacgcaa
     2401 ggtccggatg attccgagcg attgcgttct tcgcgaaact ctcgcactcg gcgggcgtcc
     2461 tcaatctctc gactcgataa tccatgtcgt ttcctcgcgg catcctagcg actcgggccg
     2521 cggcgctaag agatggcgcg cgcgaaggcc gcgacgacct cggggggtag attgccgttg
     2581 ctcgtgcgcc gaagcccatc gctgcgcacc tcgtacatcg cggacgcgta gcccgcggcg
     2641 ctcacgagct gcacgtactt gttgcgccca aagcgggcga cgcggcacgc tgcaggtttg
     2701 accacgaacg cgtttcgttc cttcgcgtgt gtcgccatga ccgttgcaac ggtttcctga
     2761 tcctcgatga aaactacagg ccgttccaag cagccgaggt gctcggcgag tgcaagaaga
     2821 gtcggaaaag agcgggtgtg ccgcttgccg tcgcgcagcg cgtagacggc ggctgttccg
     2881 cccggcagac cgtcggaccg aacgtcatag cctgcgcgcc gtatgatctt ttcgatttcc
     2941 ctatcgagca tgtttcactt gagacgcggg gcgaggccta gacccagcat cggcagatac
     3001 tcgaagccgt tgccgcgcga ccaaaggagc agcgtatcga ggcgccagaa ctcctgtttg
     3061 tacggcaaga ccggcaatgc cgttaggccg agctgctcgg cgcggttccg gcaatctcca
     3121 acgctgcgca tccccagctg ctcgggtgtg acgaccacct gccggctccg ccactcggtt
     3181 gtttgatgac cagcctcgac catgggcgcg acctgagagt gaaacacggc tccgagtcta
     3241 tgcctaagtc gacaagcgcg tactcatttc ggcgccgcgg gcggcgcgat tgccgcgcga
     3301 cgggggaaac gaacgagccc ccctcgcagt ggcgaggggg
//
LOCUS       DATLEE010000029         2804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2267434, whole genome shotgun sequence.
ACCESSION   DATLEE010000029 DATLEE010000000
VERSION     DATLEE010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267434"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..849
                     /locus_tag="VM692_00630"
     CDS             307..849
                     /locus_tag="VM692_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HUQ50691.1"
                     /translation="MKRDETTDVTNRGAARELLHLLLSQVVPAAANPRLTMEKESFQE
                     LSKSIEQNGVLQPIVVRPLGDKYEIIGGHRRHAVLVELAAKHPGDPRFTHIPAAVIEA
                     DDAQAPVLRLVENINRSDLTPLEQVEGLARAVEAGNKKEVLAAGLGWPMRKLYKYLQL
                     AEAPAWLRDHLQWKAKSSSR"
     gene            complement(1204..1518)
                     /locus_tag="VM692_00635"
     CDS             complement(1204..1518)
                     /locus_tag="VM692_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50692.1"
                     /translation="MTVVEIFYFEGCPGHAAAAELVARVVRDAALNVEVRQVEVANSV
                     AAEQLRFLGSPTVGVDGIDVDPEADQRHDFAMQCRVYAVNGRLQNTPSREWIERALGV
                     AR"
     gene            complement(1551..>2804)
                     /locus_tag="VM692_00640"
     CDS             complement(1551..>2804)
                     /locus_tag="VM692_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50693.1"
                     /translation="KGSFDDAPFHHLFRVLAEIRAASADSLDELFFQRVFLKQCRRST
                     NGMLARLVEEGFLARMDLKQARVIYHLTRKSLATIEVAGIHVPETLRRPPTDELGQVF
                     WLRASLRAEHSRLGFTVGRGPREAYALRRFLIDSVERSAASRALLAELRADPALCPPM
                     TDGCERCGFIAAYGAARAMCPRCANITSPVPLGRAFKCATCDVIAATPGPHGRCTALM
                     RELECLPVDVAWRKVGERYDVRIVFVDNPVHGLRAQLEKLPVQHMDQPKVPILLRSAD
                     PESRYDRGNAAWASKGPRHQLLDAAFSEEGLNDLWPIESTTRVIDVRPDLQLYVLRSD
                     ARAPAWDCGVLGAGQGKAPRERAKAILTERNAERLVRDREERHEVRGRVEAAKPVIRR
                     EERAIAPRTAPRIKREEERAPRRRR"
BASE COUNT          520 a          944 c          883 g          457 t
ORIGIN      
        1 gtgaggcttt cccggttctc gttcacatcg acagcttaga ggtcttgcct gattgcgcct
       61 cgcagaccga ccgagatttc ttcgaagcca ttgcggacga atcgctcctc ccctgacaca
      121 cgctgcgctt cgtgtgtcgc cgtcgtcgtc aaggcgcagc gcggcgcgac gacggcggtg
      181 acacggaggt cgcccgagtc cgagccctct cctgtacttc ggtcacgctc caacggtgga
      241 tccgatccgt ttggcatctc cccaggtagg ggccgacttt ttttcggcgc cagcaggaga
      301 tgcgagatga agcgagatga aacgaccgac gtgaccaacc gtggtgctgc gcgcgagctg
      361 ctgcacctct tgctgagcca ggtcgtcccc gcggcagcga acccgcgcct gacgatggag
      421 aaggagtcct ttcaagagct cagcaagagc atcgagcaga acggcgtgct ccagccgatc
      481 gtcgtgcgcc cgctggggga caaatacgag atcatcggcg ggcaccgccg tcacgccgtg
      541 ctcgtcgagc ttgccgccaa gcacccgggc gatccgcggt tcacgcacat ccccgcggcc
      601 gtaatcgaag cggatgacgc gcaagcgcca gtgctgcggc tcgtggagaa catcaaccga
      661 tccgatctca caccactcga gcaagtcgaa gggctcgcgc gcgcagtcga agccggcaac
      721 aagaaggagg tgctcgcagc ggggctcggc tggccgatgc gcaagctcta caagtacctc
      781 cagctcgccg aggcgcccgc ttggcttcgt gatcatttgc aatggaaagc gaagtcatca
      841 agccgttaat aatagtgaat cgaggtcaat ctgggcgcca gtgaccgatc cacaagatgc
      901 ccaatgcggc acttgcggac cggcacgcaa tggcgtgttc gcaggagcga acgggccggc
      961 aacgacgtcg actcatgggc aagagcgcgg cgacgtctgc cgagatgcgc gcaatgagct
     1021 cgccctcggc tgtcggtcac tggcgccaat gaagaaggcg agcatctaga gcaatggctc
     1081 cagacgcgcg catgcaagca atcagggaag caggatcaag aagtcaacga cagcatgcac
     1141 tgccatcatc caacgtgacg aacccatgca caggatcgcg ttcgtgcatc ggctcgcgag
     1201 caatcagcga gcaacgccca gcgcgcgctc gatccactcg cgcgacggcg tgttctgaag
     1261 cctgccgttc accgcataca cccggcactg catcgcgaag tcgtgccgtt ggtccgcttc
     1321 ggggtcgaca tcaatgccgt caaccccgac cgtgggcgag ccgagaaacc gcaactgctc
     1381 ggccgcgacg gagttcgcga cttccacctg ccgcacctcg acgttcagcg cggcatccct
     1441 cacgacgcgg gcgacgagct ccgcggcagc cgcatgtccg gggcacccct cgaagtagaa
     1501 gatctcaacg acggtcatcg ccgattgtag acgcagtcgc tcgcgcaagg tcagcgccgg
     1561 cgccgcggcg cgcgctcctc ttcccgcttg atgcgcggcg cagtccgcgg agcgatcgcc
     1621 cgctcttcac gccggatgac gggtttcgcc gcctcgacgc gtccacgcac ctcgtgacgc
     1681 tcttcgcggt cgcgcacgag gcgctctgcg tttcgctccg tcaagatcgc cttcgctctc
     1741 tcacgcggtg ccttcccttg ccccgcaccg agcacgccgc aatcccaagc tggcgcgcgt
     1801 gcgtccgaac gaagaacgta aagctgaaga tcggggcgca cgtcgatcac gcgcgtcgtg
     1861 ctctcgatcg gccagagatc gttcagccct tcctcgctga acgccgcgtc gagcagttga
     1921 tggcgcgggc ccttcgacgc ccatgcagcg ttgccccggt cgtaacggct ctcggggtcg
     1981 gcgctgcgca gaaggatcgg caccttcggt tggtccatgt gctgtaccgg gagcttctcg
     2041 agctgcgcgc gcaagccgtg tacgggattg tcgacgaaga cgatccgtac gtcgtagcgc
     2101 tcgccgacct tgcgccacgc gacgtcgacg ggcaggcact cgagctcgcg catcagagcc
     2161 gtgcatcgtc cgtgcggccc cggtgtcgcc gcaatgacgt cgcacgtcgc acacttgaac
     2221 gcgcgcccga gcggaacggg tgatgtgatg ttcgcgcaac gcgggcacat cgcccgcgcg
     2281 gcgccatacg cagcgatgaa gccgcaccgc tcacagccgt cggtcatcgg cgggcacagc
     2341 gcggggtcgg ctcgcagctc ggcgagcagc gctctgctcg ccgcgctgcg ctcgaccgag
     2401 tcgatcaaga accgtcgcaa cgcgtacgcc tcccgcggac ctcgcccgac ggtaaaaccc
     2461 agtcgagagt gctcggcgcg caggctcgca cgcagccaga acacttggcc gagctcatcc
     2521 gtcggtggcc gacgcaacgt ctctggcacg tgaatgcccg caacctcgat ggtcgcgagc
     2581 gacttgcgcg tgaggtggta gatgacgcgt gcttgcttga ggtccatgcg cgcgaggaag
     2641 ccttcctcga cgagacgcgc aagcatgccg ttcgtgctcc ggcgacactg tttcaagaac
     2701 acgcgttgaa agaacagctc gtcgagactg tccgcgctcg cagcgcggat ctcggcgaga
     2761 acgcgaaaga ggtgatgaaa cggcgcgtcg tcgaacgatc cctt
//
LOCUS       DATLEE010000030         4131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5249494, whole genome shotgun sequence.
ACCESSION   DATLEE010000030 DATLEE010000000
VERSION     DATLEE010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5249494"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1082)
                     /locus_tag="VM692_00645"
     CDS             complement(27..1082)
                     /locus_tag="VM692_00645"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ50694.1"
                     /translation="MNSILARTLVVGAFAHAPAAVAHHSTAEYDSAAFVEAEGVVTKV
                     LWQNPHVRLEISTEQSDGVAQVWQLEGQSPGDLDRARIPRDLIAVGDRVKFAGNPSTR
                     RERRMYVTNVLLEDRTEIVLRANAQPRWSPEHYLSHLQATIDPARAAADGGDGIFRVW
                     LPTASKTPDWAADPPLTPAARSAWQAFDAIRDDPVLDCKPPGMPQVSTRSGRYAIRFV
                     RLGADLVLKNEYREIDRVIHMTEPDTARRHAPSPLGWSTGKWDGDTLVVTTTDIDWPY
                     FQLYGLEGVPQSSEMTIVERFTPSPDGRELTYDITASDPRTFTRTVAAPGYRTFRWQP
                     GFEFLPQNCVLDGDREK"
     gene            complement(1101..1802)
                     /locus_tag="VM692_00650"
     CDS             complement(1101..1802)
                     /locus_tag="VM692_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HUQ50695.1"
                     /translation="MLRLGTRLRSSIAAREASMLVALLLAAGAVWLFVEVAGEVAEGE
                     TRAVDEQLLLLLRTRANPSDPVGPAWVEELARDVTALGSAGILTLITFAAAGFLVLQR
                     KRPLALYLVAAIAGGTVLSSLLKWGFARPRPELVAHGQVVYTSSFPSGHSMISAVAYL
                     TLGALLASGQKNRGMHAYLMGLAVFLTLIVGISRVYLGVHWPTDVLAGWTAGAAWALL
                     CWVVAKRLHVGGTVE"
     gene            1960..>4131
                     /locus_tag="VM692_00655"
     CDS             1960..>4131
                     /locus_tag="VM692_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ50696.1"
                     /translation="MVVAAAGGAYVWRQSHAKQEQWAVVQKYCVECHNQDDLAGNRAF
                     DRLSPNHIAADAEVWELAIRKLRGGLMPPAGGPRPDAETVESLAAWLAGEIDAAADEP
                     VAGRVAMRRLNRREYAHAIRDLLALEVDPAALLPQDNVSGYYDNNAEALQVSPAFVNQ
                     YIDAARAVALEAVGDAKALPIATTYGDPANMVISLPPDGAPGTGRQQHHLPGMPFGTR
                     GGFSVKHNFPADGEYELTIGDMALAREVPRMEFENTVVVLLDGKEFYRTNIGGEADHK
                     WIDQTLDPAVEAINNRLRKIRFNATAGQHDLAVTFVHRSFAESDERTRTIALEGGQER
                     IQGAHALQIRGPLTVTGLGESPSRNKIFVCKPTESSEERRCATEIVTQLAERAFRRPI
                     TDEDLGGLMAFYDAGARQDGFEGGVRDALSAILASPHFLYRAEAAGEGLGTLTDLELA
                     SRLSFFLWGSLPDQELLELAEASRLRDRGVLATQVKRMLADDRAKALVQDFAFQWLHV
                     SKLDEIVPDRGQFPQASGLLDPRDLMKEELSLFVDSVLRSDRSVVDLLTADYTYLNER
                     LAMHYGIESVKGARFRRVTLDDDARYGLLGKGAILMLTAYPNRTSPVLRGAWVQERLI
                     GAAVPAPPPDVPDLTENKRGEPPRTLRARLEQHRENPTCFACHGIMDPLGFALEGFNA
                     VGQKRTIDPDTRTPIDSSGTLPDGTPVTGAADLRNALITHPE"
BASE COUNT          704 a         1393 c         1400 g          634 t
ORIGIN      
        1 cagctgcgcc tacctgagcg gagccgtcac ttttcgcggt cgccatcgag cacgcagttc
       61 tgcggcaaga actcgaatcc gggttgccaa cggaacgtgc ggtagccggg cgcagcgacg
      121 gtgcgcgtga acgtgcgcgg atcgctggcc gtgatgtcgt acgtcagctc gcggccgtcg
      181 ggggacggcg tgaagcgctc gacgatcgtc atctcgctgc tctgcggcac accttcgagt
      241 ccgtagagct ggaagtacgg ccagtcgatg tcggtcgtcg tgacgaccag cgtgtcacca
      301 tcccacttgc cggtcgacca cccgagcggt gatggcgcat gcctgcgcgc cgtgtcgggc
      361 tcggtcatgt gtatgacgcg atcgatctcg cggtactcgt tcttgagcac gagatcggcg
      421 cccaggcgca cgaagcggat cgcgtaccgc ccgctgcgcg tgctgacctg cggcatgccg
      481 ggaggcttgc agtcgaggac gggatcgtcg cgaatggcgt cgaaagcttg ccaggccgaa
      541 cgcgcggccg gcgtgagcgg cggatcggca gcccagtccg gcgtcttcga tgcggtcggc
      601 agccaaacgc gaaagatgcc gtccccgccg tcggcggcgg cgcgggcggg gtcgatcgtc
      661 gcttgcagat ggctcaagta gtgctccggc gaccaacgcg gttgcgcatt ggctcgcagc
      721 acgatctcgg ttcgatcctc gagcaagacg ttcgtgacat acatgcgccg ctcgcggcgc
      781 gtcgatgggt tgccggcgaa cttgacgcga tcccctacgg cgatgaggtc gcgcggaatg
      841 cgtgcgcggt cgagatcgcc ggggctttgg ccttcgagct gccacacctg cgccacgccg
      901 tccgattgct cggtgctgat ctcgagacgc acgtgtgggt tctgccagag gactttcgtg
      961 acgacaccct cggcctcgac gaacgccgcc gagtcgtatt ccgccgtcga gtgatgcgcg
     1021 accgcggccg gcgcgtgcgc gaacgcgccg acgacgagcg tgcgagctag gattgagttc
     1081 acgatgcgca cgctcccagg ttactcgacc gtgccgccga cgtgcagccg cttcgcgacg
     1141 acccagcaga gcagcgccca agcggccccg gccgtccagc cggcgagcac gtcggtcggc
     1201 caatgcacgc cgaggtagac gcggctgata cctacgatca gagtcaggaa cacggcgagc
     1261 cccatgaggt acgcgtgcat gccgcggttc ttctggccgc tcgcgagtaa ggcgccgagc
     1321 gtcagatagg cgacggccga gatcatcgaa tgtccgctcg gaaagctgct cgtgtaaacg
     1381 acttgtccgt gcgcgacgag ctcgggccgc ggcctcgcga agccccactt cagcagcgag
     1441 ctcaataccg tgccgccggc gatcgccgcg acgaggtaga gcgcgagcgg ccgtttgcgc
     1501 tgcagcacga ggaaaccggc cgcagcgaac gtgatcagcg tgagaatgcc ggcgcttccc
     1561 aatgccgtga cgtcgcgcgc aagctcctcg acccaagcgg ggccgacagg gtcgctggga
     1621 tttgcgcgag tgcgcagcag caacagcagt tgctcgtcga ccgcgcgcgt ctcgccctcg
     1681 gcgacctcgc ccgcgacttc gacgaacagc caaacggcgc cggccgcgag cagcaacgcg
     1741 accagcatgc tggcctcgcg ggccgcaatc gagctgcgca gtcgggtccc aagtctcagc
     1801 atcggcgcag tttgcgacaa aacttaacgc aggtcgcaag cctcagacgg cgggcaccac
     1861 ctataatagg agtcgtctcg gtgccgcccg ggcagggcgg agtcgtaagt cgggggtagc
     1921 gtcatttccg ccaaggtcgt cctaaccgtt gcatccctag tggtcgtcgc cgcagccggc
     1981 ggcgcgtatg tctggcggca atcgcacgcc aagcaagagc agtgggccgt cgtccagaag
     2041 tactgcgtcg agtgccacaa tcaagacgac ctggcgggta accgggcttt cgaccggctg
     2101 tcgccgaacc acatcgccgc cgatgcggaa gtctgggagc tcgcgatccg taagctgcgc
     2161 ggcggtctga tgccgccggc cggcggcccg cggcccgacg cggagacggt cgagagcttg
     2221 gccgcgtggc tcgcgggtga aatcgacgcc gcggccgacg agcccgttgc cggtcgcgtg
     2281 gctatgcgcc ggctaaaccg gcgcgagtac gcgcatgcga ttcgcgactt gctggcgctc
     2341 gaggtcgatc ccgcagcgct gctgccgcag gacaacgtca gcggctacta cgacaacaac
     2401 gccgaagcac tgcaagtgtc gccggcgttc gttaaccagt acatcgacgc agcgcgcgcc
     2461 gtcgcgcttg aggccgtcgg tgacgccaag gcgctgccga tcgcgacgac ctatggcgac
     2521 cccgccaaca tggtcatctc attgccgccc gacggcgccc ccggcacggg tcgccagcag
     2581 caccatttgc ccggcatgcc gttcggcacc cgtggcggct tcagcgtcaa gcacaacttc
     2641 cccgccgacg gcgagtacga gctcacgatc ggcgacatgg cgctcgcgcg cgaagtgccg
     2701 cgcatggagt tcgagaacac ggtcgtcgtg ctgctcgacg gcaaagagtt ctatcgcacg
     2761 aacatcggcg gcgaagccga tcacaagtgg atcgaccaga cgctcgaccc cgccgtcgag
     2821 gcgatcaaca accggttgcg caagattcgc ttcaacgcga cggccggcca acacgatctc
     2881 gccgtgacgt tcgtccatcg cagctttgcg gaaagcgacg agcggacgcg gacgatcgcg
     2941 ctcgagggcg gccaagagcg tatccaaggc gcgcacgcgt tgcagatccg cgggccgttg
     3001 acggtcacgg gcctcggcga atcgccgagc cgcaacaaga ttttcgtctg caagccgacc
     3061 gaaagctccg aagagcggcg ctgcgcgacc gagatcgtca cgcagctcgc cgagcgcgcg
     3121 ttccgtcggc cgatcacgga cgaagacttg ggcggcctca tggcgttcta cgacgcgggc
     3181 gcgcgccagg acggtttcga aggcggcgtt cgcgacgcgc tgtcggcaat cctcgcgagc
     3241 ccgcacttcc tgtatcgcgc ggaagccgcc ggcgaaggct tgggcacgct gacggacctc
     3301 gagctcgcgt cgcgcctgtc atttttcttg tggggcagcc taccggatca ggagctgctc
     3361 gagctcgccg aggcctcgcg gctgcgcgac cgcggcgtcc tggccacgca ggtcaagcgc
     3421 atgctggccg acgaccgagc caaagcgctc gtccaggact tcgcgttcca atggctgcac
     3481 gtgtcgaagc tcgacgagat cgtgccggac cgcggccagt tcccgcaggc cagcggcttg
     3541 ctcgatccgc gcgacctcat gaaagaggag ctcagtctgt tcgtcgacag cgtgctgcgc
     3601 agcgaccgca gcgtcgtcga cttgctgacg gcggactaca cgtatctgaa cgagcggttg
     3661 gcgatgcatt acggcatcga gagcgtcaaa ggcgcgcggt tccgccgcgt cactttggac
     3721 gacgacgcgc gttacggtct gctcggcaag ggcgcgatcc tgatgctgac ggcttacccg
     3781 aaccgcacgt cgcccgtgct gcgcggcgcg tgggtccaag agcgattgat cggcgccgcc
     3841 gtgcccgcgc cgccgcccga cgtgccggat ctcaccgaga acaagcgcgg cgagccgccg
     3901 cgcacgctgc gcgcgcgact cgagcaacac cgcgagaacc cgacgtgctt tgcctgccac
     3961 ggcatcatgg atccgctcgg cttcgcgctc gaggggttca acgccgtggg gcagaagcgc
     4021 acgatcgatc ccgacacgcg cacgccgatc gactcgagcg gcacgttgcc cgacggtacg
     4081 ccggtgacgg gtgccgcgga cttgcgcaac gcgctcatca cgcatcccga g
//
LOCUS       DATLEE010000031         6826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_777408, whole genome shotgun sequence.
ACCESSION   DATLEE010000031 DATLEE010000000
VERSION     DATLEE010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777408"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /gene="rsmA"
                     /locus_tag="VM692_00660"
     CDS             complement(<1..542)
                     /gene="rsmA"
                     /locus_tag="VM692_00660"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HUQ50697.1"
                     /translation="MRAKKRLGQHFLHDPAVIRRLVDVIRPEATDTMVEIGGGPGALT
                     LPLCEKLERLHVVEVDRELAAALPSRVSDRQRLVVHEADALTFDFGALATGPRSLRVV
                     GNLPYNISTPLLFHLFEFAPVIKDLHVMLQREVVDRMTAAPGGKEYGRLTVMVGLWAR
                     AEACFDIGPGAFTPPPKVWST"
     gene            complement(549..1529)
                     /gene="pdxA"
                     /locus_tag="VM692_00665"
     CDS             complement(549..1529)
                     /gene="pdxA"
                     /locus_tag="VM692_00665"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HUQ50698.1"
                     /translation="MSKALPVVVVSSGEPAGIGPDICLALAGRPFGARLAVLGDLTLL
                     ETRARLLGLNVELARCDDVAAIEPHRTGRLQVLPLALAATAKPGALDTRNAAYVLAML
                     RRATELCLAGTSQALVTAPVQKSVITQSGVAFSGHTELLAELTGTPQPVMLLAGKSLR
                     VALATTHLPLRAVAAALDQAKLESLIRITHRDLQRRFRIARPRVLVLGLNPHAGESGT
                     LGTEELSIIEPAVRALAAEGLDITGPVSADTAFTPESLARCDVVIAMYHDQGLPVLKA
                     LSFGEIVNVTLGLPIVRTSVDHGTALSLAGTGRARPESLFAAVELALELL"
     gene            complement(1578..2936)
                     /locus_tag="VM692_00670"
     CDS             complement(1578..2936)
                     /locus_tag="VM692_00670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF020872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUQ50699.1"
                     /translation="MSSVYHRPLFSKAASAAASTALALCLSTGASAQDRELGAGGVLL
                     DGVAAVVDEGVVLKSELAERLNLVMQNLQKQQAEAPPEQRRPLPPMSAIEQQVLEQLI
                     LREIQIQRANKVGITVSDDLLNEALSRVAQNLGYTLEELPTVLASQNIDYAAYRDDSR
                     RDLLVEQLEQRDVISRIAITPREMEQCLLTSDASAADTFDYNISHILISVPANATQQD
                     IEAARKRINEIHDRLENGEDFARLAVATSQAQTALEGGSLGWRKGAQLPTLFAPYVTK
                     MKPGDYSEPIQNSGGFQIVRLNEMRGAERTMVDQLHVRHILLRPNEILDESATQQKLL
                     GIRAQIENGDDFATVAEAVSNDTQSANQGGDLGWVSPGETVPEFEQALAELPLNELSQ
                     PIKSRFGWHLVQVLERRSHDTTDEVKREQCARQIRATKAEEESELWARRLRDQAFVDI
                     RR"
     gene            complement(2950..5172)
                     /gene="lptD"
                     /locus_tag="VM692_00675"
     CDS             complement(2950..5172)
                     /gene="lptD"
                     /locus_tag="VM692_00675"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HUQ50700.1"
                     /translation="MNQPHLVLAASHRSIIAFWLVVGLAPQSFAQDLCLVPSDPFLDL
                     PPAPTFEGDEPIEITGRRFALGADNELEFEGEVVIRQGNRSITAENARRDENGDFEAL
                     GRVNLVDPEVTVFGEDAHYDSESETISFSAAGFDLPKRPARGSADQIEIRSDSRVSLA
                     NVLFTTCPKENVAWELSARDIELDVNGGIGKARGVKLDFKGVPILYAPYFTFPINDAR
                     KSGFLTPDISSRDRTGFDLSVPYYLNLAQNYDLTLEPRYMSKRGTQARGDFRYLLRNS
                     RGEFGFEYLPDDDETQSTRRYVNMQHESLFGMRDQLQVLAGIEEVSDEQYFEDLGSSL
                     SVTSQTHLNRFLDLTFFAPNWSVLTRFQNYQTIDPVLTDLERPYERTPQIVFDGRWLG
                     NLLAFDSNTELVNFERNIGTTGWRLDSTQELSLRFARAGMFLTPAIALRQTNYWIDDP
                     APGEDDRKSRSLPIGSLDMGLKFERDATRGTRTWAQTLEPRLLYVRVPFEDQSDLPVF
                     DTILPDFNLIQLFRKYQFVGPDRIADTDQLSFGVTTRLIDSANGRERMTATLGQTRYL
                     NAQRVTLPGTSAADTNASDYVAEVGIGLRDSWALDMGYQWNSQTSSTARTETRLEYRP
                     QEDRLFGMGYRYRRGALEQGDVSLVWPVSQRWRIIGRYSYSFLDKERLEDFFGWEYEA
                     CCWRLRMVNRNYVSRRTGEIDNSISLQLELKGLSQRVTAPDDLLERGILGYRNIARAY
                     "
     gene            5299..5964
                     /locus_tag="VM692_00680"
     CDS             5299..5964
                     /locus_tag="VM692_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HUQ50701.1"
                     /translation="MRAMILAAGRGERMGALTAMQPKPLLTIGDRPLIEHHVARLAAS
                     GVDEIVINLSYRGTQIREQLGNGERYGVTIAYSEEGEPPLETGGGIVRALPLLGHEPF
                     VLVNSDVFTDFDFRVLVAGPRVPTLLLVPNPTHNARGDFGLDAAGFVSSAAPLKTYAG
                     IAVFDTRMFASLAPGRRPLKPLLDAAIARRELRGLEHAGVWLDVGTPERLEQARAVAA
                     RRR"
     gene            5964..6599
                     /gene="maiA"
                     /locus_tag="VM692_00685"
     CDS             5964..6599
                     /gene="maiA"
                     /locus_tag="VM692_00685"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HUQ50702.1"
                     /translation="MRLYSFFRSSAAFRVRIALNLKGVDYETVAVNLPGGAHRTADFL
                     AVNPQATIPTLDDGGTILWQSLAIIEYLDAKFPAPKLIPAEPVARARVQALAQLIACE
                     IHPLNNLRVLKYLRGELKLDEAAVSKWYSHWVAEAFAPLESFVARWSGGRYCFGDALT
                     IADVYLVPQMYNARRFNCDVAPYPTLVRIAAQLEQEPAFARAAPERQPDAV"
     gene            complement(6615..>6826)
                     /locus_tag="VM692_00690"
     CDS             complement(6615..>6826)
                     /locus_tag="VM692_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008884454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HUQ50703.1"
                     /translation="DGAAAHADEMPLVELQPSPEFTKVAEASDAWAKRVDDPAELPRA
                     IDAALKAVAGGRQALLDVRMEHGVR"
BASE COUNT         1103 a         2219 c         2319 g         1185 t
ORIGIN      
        1 gtcgaccaca ctttcggcgg cggcgtgaac gcgccgggcc cgatgtcgaa gcaggcttcg
       61 gcacgtgccc agagacctac catgaccgtg agacgaccgt actccttgcc gccgggtgcc
      121 gccgtcatgc gatcgacgac ctcgcgctgc agcatgacgt gcagatcctt gatgacgggc
      181 gcgaactcga acaggtggaa cagcagcggc gtcgagatgt tgtagggcag gttgccgacg
      241 acgcgaagcg agcgcggacc cgttgcgagc gcaccgaagt cgaacgtcag cgcatcggct
      301 tcgtgcacga cgagccgctg ccggtcggac acgcggctcg gcagagccgc cgcgagctcg
      361 cgatcgactt cgacgacgtg cagacgctcg agcttctcgc acagcggaag cgtcagcgcg
      421 ccggggccgc cgccgatttc gaccatcgtg tcggtagcct cgggacggat gacgtcgacg
      481 agccggcgga tgacggccgg atcgtgcaag aagtgttggc cgagccgctt cttggcgcgc
      541 acggcgagct aaagcagctc gagcgcgagc tcgacggccg cgaacagact ctcggggcgc
      601 gcgcgtcccg tgcccgccag cgacaacgcc gtgccatggt cgaccgacgt gcgcacgatc
      661 ggcagcccta gcgtcacgtt gacgatctcg ccgaacgaca acgccttcag cacgggcaga
      721 ccttgatcgt gatacatggc gattacgacg tcgcaacgcg cgagcgactc cggcgtgaac
      781 gccgtgtcgg ccgacacggg acccgtgatg tcgagccctt cggcggcgag cgcgcgcacc
      841 gcgggctcga tgatagaaag ctcctcggtg ccgagcgtgc cgctttcgcc ggcgtgcgga
      901 ttcaagccga gcacgagaac gcgcggccgc gcgatgcgaa accgccgctg caagtcgcgg
      961 tgcgtgatgc gaatcaagct ctcgagcttt gcttggtcga gcgccgccgc gacggcgcgc
     1021 agcggcaggt gcgtcgtcgc gagcgcgacg cgcagcgact tgccggcaag cagcatgacg
     1081 ggctgcggcg tgcccgtgag ctcggcgagc agctccgtgt ggcccgagaa ggcgacgccc
     1141 gattgcgtga tgacgctctt ttgcaccggg gccgtcacga gggcttggct cgtgcccgcg
     1201 aggcagagct ccgtggcgcg gcgcagcatc gcgagcacgt acgcggcgtt gcgcgtgtcg
     1261 agcgcgccgg gtttcgcggt agcggcgagc gcgagcggta gcacctgcaa acggccggtg
     1321 cggtgcggct cgatggccgc cacgtcgtcg caacgagcga gctcgacgtt caggccgagc
     1381 aggcgcgcgc gcgtttcgag caacgtcaag tcgccgagca cggcgaggcg cgctccgaac
     1441 gggcgccccg cgagcgccaa gcagatatcc ggaccgatgc cggccggctc gccggagctg
     1501 acgacgacga caggtagtgc tttgctcacg ctatgccgcc cgatgcggcg gagagagtcg
     1561 gcctctcgaa gccccgctca gcggcggata tcgacgaacg cttggtcgcg gagccgccgt
     1621 gcccaaagct cggactcttc ctcggccttg gtcgcgcgaa tttgccgcgc gcactgctcg
     1681 cgcttaactt cgtccgtcgt gtcgtgcgac cggcgctcga gcacctgtac gaggtgccaa
     1741 ccgaagcgcg atttgatcgg ctggctgagc tcgttcaacg gcaattcggc cagcgcttgc
     1801 tcgaactcgg gcacggtctc gccgggcgac acccagccca aatcgccgcc ctggttggcg
     1861 gactgcgtat cgttcgacac ggcttcggca accgtcgcga aatcgtcgcc gttctcgatc
     1921 tgcgcacgga tcccaagcag cttttgctgg gtcgccgact cgtcgaggat ctcgttcggc
     1981 cgcagcaaga tgtggcgcac atgcagctgg tcgaccatcg tgcgctcggc gccgcgcatt
     2041 tcgttcaagc gcacgatctg gaacccgccg ctgttttgga tgggttccga gtagtcgccg
     2101 ggcttcatct tcgtgacgta cggcgcgaac agcgtcggca actgcgcgcc cttgcgccag
     2161 cccaagctgc cgccctcgag cgccgtttgc gcctgcgacg tggcaaccgc gaggcgcgcg
     2221 aaatcttcgc cgttctcgag ccggtcgtgg atctcgttga tgcgcttgcg cgcagcttcg
     2281 atgtcctgct gcgtcgcatt cgccggcacg ctgatcaaga tgtgcgagat gttgtaatcg
     2341 aacgtatcgg ccgcgctggc atccgacgtg aggaggcact gttccatctc gcgcggcgtg
     2401 atggcgatgc ggctgatgac gtcgcgctgc tcgagttgct cgacgagcaa atcgcggcgg
     2461 ctgtcgtcgc ggtacgctgc gtagtcgatg ttctgcgacg ccagcacggt cggcagctct
     2521 tcgagcgtgt agcccaggtt ctgcgcaacg cgcgacagag cctcgttgag caggtcgtcg
     2581 ctgacggtga tgccgacttt gttggcgcgt tggatctgaa tctcacgcag aatcagctgc
     2641 tcgagcacct gctgctcgat ggcggacatc ggcggcaagg gccgacgctg ctcgggcggc
     2701 gcttcggcct gctgcttctg caggttttgc atcacgaggt tcagccgttc ggcgagctcg
     2761 ctcttcaaca cgacgccttc gtcgacgacg gcggccacgc cgtcgagcaa cacgccgccg
     2821 gcaccgagct cgcgatcctg cgcgcttgcg ccagtggaca ggcacagcgc gagcgcggtg
     2881 ctcgcggcag ccgacgctgc tttcgaaaac agaggacggt ggtaaacgct gctcattaaa
     2941 tcgctcttct cagtaggcgc gggcgatgtt gcgatagccc agaataccac gctcgagcag
     3001 gtcgtccggc gccgtgaccc gctgcgacaa gcccttgagc tcgagctgca gcgagatcga
     3061 gttgtcgatc tcgcccgtgc ggcggctgac gtagttgcgg ttgaccatgc gcaggcgcca
     3121 gcagcacgcc tcatactccc agccgaagaa atcctcgagc cgctccttgt cgaggaacga
     3181 gtagctgtag cgcccgatga tccgccagcg ctgggagacg ggccacacga gcgagacgtc
     3241 gccttgctcg agcgcgccgc gacggtagcg gtagcccatg ccgaacagcc gatcctcctg
     3301 cggccggtat tcgagccgcg tttccgtgcg tgccgtcgag gatgtctggc tgttccattg
     3361 gtaacccatg tcgagcgccc acgagtcccg caagccgatg ccgacttctg cgacgtagtc
     3421 cgaggcattc gtgtccgccg ccgacgtgcc gggcagggtg acgcgctgcg cgtttagata
     3481 gcgcgtctgg cctaaagttg ccgtcattcg ctcgcggccg ttggcggaat cgatgaggcg
     3541 cgtcgtgacg ccgaacgaca gctggtccgt atcggcgatg cggtcgggcc ctacgaactg
     3601 gtacttgcga aacagctgga tcaagttgaa gtccggcagg atcgtgtcga acaccggcag
     3661 atcgctctga tcctcgaacg gcacgcggac atagaggaga cgcggctcga gcgtctgcgc
     3721 ccaggtgcgg gtgccgcgcg tcgcgtcgcg ctcgaacttc aggcccatgt cgaggctgcc
     3781 gatcggcaaa ctgcgcgact tccggtcgtc ctcgccgggc gccgggtcgt cgatccaata
     3841 attggtttgc cgcagcgcga tcgccggcgt caggaacatg ccggcccgcg cgaagcgcaa
     3901 gctcagctcc tgcgtcgagt cgagccgcca ccccgtggtg ccgatgttgc gctcgaagtt
     3961 cacgagctcg gtgttcgagt cgaatgcgag caagttgccg agccaacggc cgtcgaagac
     4021 gatctgcggc gtgcgctcgt acgggcgctc gagatcggtc agcacggggt cgatcgtctg
     4081 gtagttctgg aaacgcgtca gcaccgacca gttaggcgcg aagaacgtaa ggtcgaggaa
     4141 acggttcagg tgtgtctggc tcgtgaccga caagctgctg cccaggtctt cgaagtactg
     4201 ctcgtccgag acctcctcga tgcccgcgag cacttgcagc tgatcgcgca tgccgaacag
     4261 cgactcgtgc tgcatgttga cgtagcgccg cgtgctttgt gtctcgtcgt cgtcgggcaa
     4321 atactcgaag ccgaactcgc cgcggctgtt gcgcagcaga tagcggaaat cgccgcgtgc
     4381 ttgcgtgccg cgcttgctca tgtagcgcgg ctctagagtc aagtcgtagt tctgcgccag
     4441 attcaggtaa tacggcacgc tcaagtcgaa gcccgtgcgg tcgcggctgc tgatgtcggg
     4501 cgtcaggaag ccgctcttgc gcgcgtcgtt gatcggaaac gtgaagtacg gagcgtagag
     4561 gatcggcacg cccttgaagt cgagcttgac gccgcgcgcc ttgccgatgc cgccgttgac
     4621 gtcgagctcg atgtcgcgcg cgctgagctc ccacgcgacg ttttccttcg ggcaggtcgt
     4681 gaacagcacg ttggcgagcg acacgcggct gtcgctcctg atctcgatct ggtcggccga
     4741 gcccctcgcg gggcgtttgg gcaagtcgaa gccggccgcg ctgaagctga tcgtctcgct
     4801 ctccgagtcg tagtgcgcgt cctcgccgaa cacggtgact tcgggatcga cgagattgac
     4861 gcggccgagc gcttcgaagt cgccgttctc gtcgcgcctc gcgttctccg ccgtgatcga
     4921 gcgattccct tgcctgatca cgacttcgcc ttcgaactcg agctcgttgt cggcgcccaa
     4981 ggcgaagcgt cgacccgtga tctcgatcgg ctcgtcgcct tcgaatgtcg gtgccggggg
     5041 cagatccaaa aatggatcgc tcggaacgag gcaaagatct tgggcgaagc tttgcggcgc
     5101 caaaccgacg acgagccaga acgcgatgat cgatcggtgg cttgcggcga gcacgagatg
     5161 cggctgattc aaaggcagtt acaatggcat agcgattgcg tcgcttcaac ggcttgcctg
     5221 gattgctgca cgtgttggtt gagatcgaga atcgcggccg aagtgaaccc cggacgtgag
     5281 ggataggttc ggtagcgcat gcgagcaatg atcctcgcgg cagggcgcgg cgaacgcatg
     5341 ggcgcactga cggcgatgca accgaagccg ttgctgacga tcggcgatcg cccgctcatc
     5401 gagcaccatg tcgcgcgtct cgcggcgagc ggagtcgacg agatcgtcat caatctatcg
     5461 tatcgaggca cgcagatccg cgagcagctc ggcaacggcg agcgctacgg cgtcacgatc
     5521 gcgtacagcg aggaaggcga gccgccgctc gagacgggcg gcgggatcgt gcgcgcgctg
     5581 ccgttgctcg gccacgagcc gttcgtgctc gtgaactccg acgtgttcac cgatttcgat
     5641 tttcgcgtgc tcgttgcggg cccgcgcgtg ccgacgctcc tgctggtgcc gaacccgacg
     5701 cataacgcgc gcggtgactt cgggctcgat gcggcgggct tcgtgtcctc agcggcgccg
     5761 ctgaaaacct atgcgggcat cgccgtgttc gacactcgaa tgttcgcgtc gttagcgccg
     5821 ggccggcggc cgttgaagcc gttgctcgat gccgccatcg cgcggcgcga gctacgcggc
     5881 ctcgagcacg cgggcgtgtg gctcgacgtg gggacccccg agcggctcga gcaggcgcgc
     5941 gccgtggccg cgcggaggcg atgatgcgtc tttactcttt cttccgcagc tcggccgcgt
     6001 tccgcgtgcg gatcgcgctg aacctcaagg gcgtcgacta cgagaccgtg gccgtcaacc
     6061 tgccgggcgg cgcacaccgc acggcggact tcttggccgt gaatccgcaa gcgacgattc
     6121 cgacgctcga cgacggcggc acgatcctct ggcagtcgct ggcgatcatc gaatacctcg
     6181 acgcgaagtt tccggccccg aagctgatac cggccgagcc cgtcgctcgc gcgcgcgtcc
     6241 aagcgctcgc gcagctgatc gcgtgcgaga tccatccgct gaacaacttg cgggtgctga
     6301 agtatctgcg cggcgagctc aagctcgacg aggccgccgt cagcaagtgg tactcgcact
     6361 gggtggcgga agccttcgcg ccgctcgaga gcttcgtggc gcggtggagc ggcggccgct
     6421 attgcttcgg cgatgcgttg acgatcgccg acgtctatct cgtgccgcag atgtacaacg
     6481 cgcggcgctt caactgcgac gtcgcgccat atccgacgct cgtgcgcatc gcggcgcagc
     6541 tcgagcaaga gccggcgttc gcgcgcgcgg cgcccgagcg ccaacccgac gcggtctgag
     6601 accgccgcag ccgctcagcg gacgccgtgc tccatgcgca cgtcgagcag cgcttgccgg
     6661 ccgccggcta ctgctttcag cgcggcgtcg atcgcgcgcg gcagctcggc cggatcgtcg
     6721 acgcgcttcg cccatgcatc ggaagcctcg gcgaccttcg tgaactccgg cgacggctgc
     6781 agctcgacga gcggcatctc gtcggcgtgc gccgccgcgc cgtcgg
//
LOCUS       DATLEE010000032         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9704720, whole genome shotgun sequence.
ACCESSION   DATLEE010000032 DATLEE010000000
VERSION     DATLEE010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9704720"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VM692_00695"
     CDS             complement(<1..1061)
                     /locus_tag="VM692_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUQ50704.1"
                     /translation="MDTLTQPKIANSHPLAGATMSGADIIIQVLADEGVGTIFGYSGG
                     AILPTYDAVFRFNQANTRNGAEPMPLIVPANEQGAGFMAAGYARASGRVGVVMVTSGP
                     GATNCVTPVRDCMADSIPIVVICGQVPTAAIGSDAFQEAPVTAVMGSVAKHVFLVTDP
                     LRLESTMRTAFEIARTGRPGPVVVDVPKDVQNWMGTFVGENQLPIPGYRQRLAAVEGN
                     ALSDASCQQFFSMLADSKRPLIYAGGGVINANASGALRAFSNALKIPVASTLMGLGGF
                     DTKHPLSLHMLGMHGTASANYAVDDCDLLIAIGARFDDRVAGVPAKFAPKAKRVLHFD
                     IDPSEIHKVKKADWHHVGLL"
     gene            1200..1697
                     /gene="ilvN"
                     /locus_tag="VM692_00700"
     CDS             1200..1697
                     /gene="ilvN"
                     /locus_tag="VM692_00700"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HUQ50705.1"
                     /translation="MRHIISILLQNEAGALARVANLFSSRGYNIESLNVAPTADETVS
                     RLTLVTTGSETVIDQIEKQLGKVVDVVDIADMTKSDHIERELALFKLHVANGSRAKLT
                     PIIEQFGARVLSDAGEQYTVELVGGSQDIDRFLKRTAQVADVITLVRSGAMAVMRGTA
                     AFKRG"
     gene            1735..>2868
                     /gene="proB"
                     /locus_tag="VM692_00705"
     CDS             1735..>2868
                     /gene="proB"
                     /locus_tag="VM692_00705"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HUQ50706.1"
                     /translation="MSTAANVTHTERLANARRIVIKIGSALFIDQQTGAMNRGWLEGV
                     CADVADLRRSGKDVVIVSSGAVALGRRELKIEGRLQLEGKQAAAAAGQIVLAHAYQEI
                     LRGFGITTAQVLLTLDDSERRELYLNASRTLLTLLGYGAVPVINENDTVATQELRYGD
                     NDRLSARVAQMVSAECLVVLSDVDGLYTADPRRDSNARYVDEVTEVTREVLDMAGGTG
                     SSHGSGGMRTKLEAARIAVGAGCRMCIATGHVARPISALLAGGKATWFLPSATPGAAR
                     KQWIAGTLKPKGTLRVDAGAERALVGGRSLLPAGVTAVEGLFGRGDNVSVVAADGREV
                     ARGLTAYPADEAKLIAGRRSGDIQEILGYSGRDEIIHRDDLVLL"
BASE COUNT          495 a          909 c          977 g          487 t
ORIGIN      
        1 agcaacccga cgtgatgcca gtctgctttc ttgaccttgt gaatctcgga cgggtcgatg
       61 tcgaagtgca ggacgcgttt agctttcggc gcgaacttgg ccgggacacc cgcgacgcga
      121 tcgtcgaagc gcgcgccgat cgcgatcagc aggtcgcaat cgtcgacggc gtagttggcg
      181 ctcgccgtgc cgtgcatgcc gagcatgtgc agcgacaacg gatgcttcgt atcgaagccg
      241 ccgaggccca tcagcgtgct cgcgactgga atcttcaaag cgttcgaaaa cgcgcgcagc
      301 gcgccgctgg cattggcgtt gatgacgccg ccgcccgcat agatcaacgg ccgcttgctg
      361 tcggcgagca tcgagaagaa ttgctggcag cttgcgtcgc tgagcgcgtt gccttcgacg
      421 gcggcgaggc gctggcgata gcccggaatc ggcagttggt tctcgccgac gaacgtgccc
      481 atccaattct gcacgtcttt gggcacgtcg acgacgacgg ggcccgggcg ccccgtgcgc
      541 gcgatctcga acgccgtgcg catcgtggat tcgaggcgca gcgggtccgt cacgaggaag
      601 acgtgcttcg ccacggagcc catgacggcc gtgacgggtg cctcttggaa cgcgtcgctg
      661 ccgatcgcgg ccgtcggcac ctgaccgcag atgacgacga ttggaatcga atcggccatg
      721 cagtcgcgga cgggcgtcac gcagttcgtg gcgccggggc ccgacgtcac catgacgacg
      781 ccgacgcggc cgctcgcgcg cgcgtagccc gcggccatga agccggcgcc ttgctcgttc
      841 gcggggacga tcaacggcat cggctcggcg ccgttgcgcg tgttggcctg gttgaagcgg
      901 aacacggcgt cgtaggtcgg caagatcgcg ccgccgctgt agccgaaaat cgtgcctaca
      961 ccctcgtcgg ccagcacctg gatgatgatg tccgcgccgc tcatcgtggc gccggcgagt
     1021 gggtgcgagt tcgcgatttt gggctgagtt agagtgtcca tggtgcgttg tcggggagct
     1081 atcgacgggc tggaagccgc taaagcacaa ggagttttga cgaaaaggga gtgcattgca
     1141 atagccgttg cactacgcca acatgtcagg ccctgcgctc gccgcccctc ccgccatcga
     1201 tgcgacatat catttcaatt ctcttgcaga acgaggcggg cgcacttgcg cgcgtcgcga
     1261 atcttttctc ttcgcgtggc tacaacatcg agagcttgaa cgtcgcgccg acggccgacg
     1321 aaaccgtatc gcgtctgacg ctcgtcacga cgggctcgga gaccgtcatc gatcagatcg
     1381 agaagcagct cggcaaagtc gtcgacgtcg tcgacattgc cgacatgacg aagtcggacc
     1441 acatcgagcg cgagctggcg ttgttcaagc tgcacgtggc gaacggctcg cgcgccaagc
     1501 tgacaccgat catcgagcaa ttcggcgcgc gcgtgctgag cgatgccggt gagcagtaca
     1561 cggtcgagct cgtcggcggc agccaggaca tcgatcgctt cttgaagcgc acggcgcagg
     1621 tcgccgacgt cataacgttg gttcgcagcg gcgccatggc cgtgatgcgc ggtaccgccg
     1681 cgttcaagcg gggctaatcc ctcgccgctc gctcgaatgg cggcggagcg catcatgagc
     1741 actgcagcga acgtgacgca caccgaacgt ctcgccaacg cgcgccgcat cgtcatcaag
     1801 atcggctctg cgctgttcat cgatcagcag accggcgcca tgaatcgcgg ctggctcgaa
     1861 ggcgtgtgcg cggacgtcgc cgatctgcgt cgttccggca aggacgtcgt gatcgtgtcc
     1921 tcgggcgccg tcgcactggg gcggcgcgag ctcaaaatcg aggggcgctt acagctcgag
     1981 ggcaagcaag cggcggcagc ggcgggccag atcgtgctcg cgcacgccta tcaagagatc
     2041 ctgcgcggct tcggcatcac gactgcgcaa gtcttgctga cgctcgacga cagcgagcgg
     2101 cgcgagctct atttgaacgc gagcagaacg cttttgactc tgctcggcta tggagccgtg
     2161 cccgtcatca acgaaaacga taccgtcgca acgcaggagc tgcgttacgg cgacaacgat
     2221 cgtctctcgg cgcgcgtcgc gcagatggtc agcgccgaat gcctcgtcgt gttgtcggac
     2281 gtcgacggtt tgtacacggc ggatccgcgc cgcgactcga acgcgcgcta cgtcgacgaa
     2341 gtgacggagg tcacgcgcga ggtgctcgat atggccggcg gcacgggctc gagccacggc
     2401 tccggcggaa tgcgcacgaa gctcgaagcg gcccgcatcg ccgtcggcgc cggttgccgc
     2461 atgtgcatcg cgacagggca tgtcgcgcgc ccgatcagcg cgctgctcgc cggcggcaag
     2521 gcgacctggt ttctgccgag cgcgacgccg ggagccgcgc gcaagcagtg gatcgcgggc
     2581 acgctgaagc cgaaaggcac gctgcgcgtc gatgccggag ccgagcgcgc gctcgtcggc
     2641 ggacgtagcc tgctgcccgc cggcgtgacg gccgtcgagg ggctattcgg gcgcggcgac
     2701 aacgtcagtg tcgtggcggc cgatggccgc gaggttgcgc gcggtctcac ggcttatccc
     2761 gccgacgaag ccaagctgat cgcgggccgt cgctccggcg acatccagga gattttggga
     2821 tacagcggtc gcgatgagat cattcaccgc gacgacttgg ttcttctc
//
LOCUS       DATLEE010000033         2413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4503846, whole genome shotgun sequence.
ACCESSION   DATLEE010000033 DATLEE010000000
VERSION     DATLEE010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4503846"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..>2413)
                     /locus_tag="VM692_00710"
     CDS             complement(292..>2413)
                     /locus_tag="VM692_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HUQ50707.1"
                     /translation="GRRASDYIDPVDELQLQATLADIPFGTDLASPVTLRWRHRNGKT
                     RWLERAMVALRDADGAWRGVRGIDRDVTLRMAQEVRIRRLNRAQRFLSGASEAAMRVR
                     DRDRLIKEACRLAVSVGGYARATIYLLPNDVAGTTPLVCSYGDVQDDGAKWSITHDLP
                     QNMGPVTQVLATAEPIVLNDLSDPADLGRLGKCAVPTDSRSRIALPLSIDLAVIGVVE
                     LDGKEAGVFGDAELALLKQVAANITFALQYLRSKESAEYLEYYDPLTGLPNRVLYLTH
                     VQSALESAKRAGRRLTVAVIDIVEFGIVNDNLGHHVGDSLLRLVAERLNGAFRDAGAV
                     CRLSDDRFSVMELDPGPDSDDSLTKRIAECFEAPFVLQGQELRVAVRTGLVEFPEDSD
                     HAEALLQQAKVALQHAKNTGARHLRYNAGMNADASQRFMLTNELRRAVAERKFALNYQ
                     PKLNLVTGLVEGVEALLRWPGEREPVPPVVFVPMLESLGLIDEVGSWVIVKAMTETAQ
                     WNANGEFRVAANVSPLQLNREDFAAGVLSSLAELGGDPRRLELEVTESSLMSDPRRAS
                     VSLAKLRAAGVSIAIDDFGTGHSSLRMLAGLPIDVLKIDGSFVRDLDTNRNHRAIVQT
                     TIRLAASFGMKTVAEGVETQEQLELLRDLGCDAVQGYLVSRPASAADLAHWFTGNTFT
                     KLKQLVAGSPVQSQPAQRAPKTRR"
BASE COUNT          388 a          801 c          807 g          417 t
ORIGIN      
        1 cttacggcga accagccgcc tgactccgtc gtagtcgcgt tgccgtgtcc tgtgcaacgc
       61 ggtcggcgag acggggcagc gcttgcacac ccgcaggaat tcgagggaac cggcatcggc
      121 ctcgcgaccg tgcggcgcat cgtggagcgg cacggcggcg cgtctcggcg caaggtcaag
      181 tcgactcggg cgccaccttc tactctactt tgggctccaa ggagctcggc caatgagctc
      241 tacggcaggt caacggccgt tatggcccgg tcatgatcga ggcctaggag cctaacggcg
      301 cgtcttaggt gcgcgctgcg ccggctgcga ttgcaccggg ctgcccgcga ccaactgctt
      361 gagcttagtg aacgtgttgc ccgtgaacca gtgcgcgaga tccgcggcgc ttgcggggcg
      421 gctcacgaga taaccctgca cggcatcgca gccgaggtcg cgcagcagct ccagctgctc
      481 ctgcgtctcg acgccttccg cgacggtctt catcccgaac gatgcggcga gtcggatcgt
      541 cgtctgcacg atcgctcggt gattgcggtt cgtgtcgaga tcgcgcacga agctgccgtc
      601 gatcttcagc acgtcgatcg gcagtccggc cagcatacgc agcgacgaat gtccggtgcc
      661 gaagtcgtcg atcgcgatcg acacaccggc ggcccgcagc ttggcgaggc tcacgctggc
      721 gcgtcgcggg tcggacatga ggctgctttc cgtgacctcg agctccagcc ggcgcggatc
      781 gccgccaagc tcggcgagcg agctcaagac gccggcagcg aagtcctcgc ggttcagctg
      841 tagcggcgac acgttcgcgg cgacgcgaaa ctcgccgttg gcattccact gcgccgtttc
      901 ggtcatcgcc ttcacgatga cccagctgcc gacctcgtcg atgaggccca gcgattcgag
      961 catcggcacg aacaccaccg gcggcacggg ctcacgctct ccgggccaac gcagcagcgc
     1021 ttcgacgccc tcgacgagac ccgtcacgag gttcagcttc ggttgatagt tcagcgcgaa
     1081 cttgcgctcg gcaacggcgc ggcgtagctc gttggtcagc atgaaccgct gcgaggcgtc
     1141 ggcgttcatg ccggcgttgt agcgcaggtg tcgtgcgccc gtgttcttcg cgtgctgcaa
     1201 ggcgaccttt gcttgctgca acaaagcttc cgcatggtcg ctgtcctccg ggaactcgac
     1261 gagccccgta cgaaccgcga cgcgcagctc ttgcccttgc agcacgaacg gcgcttcgaa
     1321 gcactcggcg atgcgcttcg tgagactgtc gtccgagtcc ggccccggat cgagctccat
     1381 gaccgaaaac cggtcgtcgc tcaagcggca gacggctccc gcatcgcgga acgcgccgtt
     1441 caagcgttcc gcgacaagcc gcagcaacga gtcgccgacg tggtggccga gattgtcgtt
     1501 gacgatgccg aactcgacga tgtcgatgac ggcgaccgta aggcgccgcc cagcgcgttt
     1561 cgccgattcg agcgccgact gcacgtgggt caaataaagg actcgattcg gcaggcccgt
     1621 cagcgggtcg tagtactcga ggtactcggc gctttccttg ctgcgcagat attgcagcgc
     1681 gaacgtgatg ttggcggcga cttgcttcag tagcgcgagc tccgcgtcgc caaacacacc
     1741 ggcttctttg ccgtcgagct cgacgacgcc gatgacggcg agatcgatac tgagcggcaa
     1801 tgcgatccgc gaccgcgagt cggtcgggac ggcgcacttg ccgagccggc cgaggtccgc
     1861 agggtcgctc aagtcgttga gcacgatcgg ctcggccgtg gcaaggacct gagtgacggg
     1921 gcccatgttc tgcggcaggt catgcgtgat cgaccacttg gcgccgtcgt cctgcacgtc
     1981 gccgtacgag cagacgagcg gcgtcgtgcc cgcgacgtcg ttcggcaaca gatagatcgt
     2041 ggcgcgtgcg tagccgccga cgctgaccgc gagacggcac gcttccttga tcaggcggtc
     2101 gcgatcgcgg acgcgcatcg ccgcttcgct ggcgccgctc aagaagcgct gcgcccggtt
     2161 caggcggcgg atgcggactt cctgcgccat gcgcagcgtc acgtcgcgat cgatgccgcg
     2221 cacgccgcgc cacgctccgt cggcgtcgcg caatgcgacc atcgcgcgct cgagccagcg
     2281 cgtcttgccg ttacggtggc gccagcgcag cgtcacgggg ctcgccaagt cggtgccgaa
     2341 tgggatgtcg gccagcgttg cttgcaattg caattcgtcg acggggtcga tgtagtcgga
     2401 ggcgcggcgg ccg
//
LOCUS       DATLEE010000034         3485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5250479, whole genome shotgun sequence.
ACCESSION   DATLEE010000034 DATLEE010000000
VERSION     DATLEE010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5250479"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VM692_00715"
     CDS             complement(<1..611)
                     /locus_tag="VM692_00715"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HUQ50708.1"
                     /translation="MNERRAQRTVAPTDSLPRAPAGGGTADAFISFARATLQREAQAL
                     ADSRPAGERPPTPDDIHRLRVAARRLRVAFKLFARFLPRHEVARFGADLRWFATSLGD
                     VRDLDVYADNFKAYARTLPAQQRSELSGYELYLRRERAAARERATAAFDSARTAALFA
                     DLARFVAEPASSGALRRWRALTTADGSRQTVRRSVGRVRRLGN"
     gene            741..1604
                     /locus_tag="VM692_00720"
     CDS             741..1604
                     /locus_tag="VM692_00720"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HUQ50709.1"
                     /translation="MEIIGVYNFKGGVGKTTTAVNLAYRSAAEDWPTVLWDLDSQGAA
                     TYCLRRDAQETGIAKELVRGDAALRDVVVATEHERLDLIPADFSYRRMDVHLDKRQDA
                     TTLLLKLMRPLQARYACLILDCPPGMSLVAENVMHAADALVVPVLPTPLSARMLEQLF
                     QFFEARGWRDVNVLPFFSMVDRRRALHKQTIDELRARFPTMLETEVPYGSEFERMTAR
                     RAPVESYAPASAAAEIYRSLWREIDARLSSLSSARAAKGTPNALPAPATEAGGAATDA
                     DLVGSPWSGGR"
     gene            complement(1618..3381)
                     /locus_tag="VM692_00725"
     CDS             complement(1618..3381)
                     /locus_tag="VM692_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ50710.1"
                     /translation="MRLPANTTMTAVCAAVLGALACTAATAQQQQQRTLRPVTGVMLQ
                     NPDAADWLSWRRTLNHWGYSPLNEINKRNVAQLKLVWTRPIAQGVQEGTPLVHDGVLF
                     FPNPNDITQAIDAATGDLIWEYRRPVQDDNADYIPFPSINRNLAIYGNLILDNGADNF
                     AYALDARTGALAWETKILDYKHGAQHSSGPIVANGKVISGRSCEPEGGPEACVLTAFD
                     ALTGKEVWRTRTIPRPDEPGGDTWGNIPYEDRRHVGLWMVPSFDPELNRIYVGTSVTS
                     PAPKFMLAGNDKQYLYHNSTLSIDADTGKIVWYLQHNVDHWDLDHPFERILIDTVVAP
                     DANAVAWINPRVRAGQRYKVVTGVPGKTGLVYTLDRETGQFLWARPTVEQNVIKSVNT
                     ESGQGVVNEEKMFTGPNQEILVCPSLTGGKNYHAGTYSPDTKLLYMPLQNVCMLSTST
                     AARPSLDELYAIRTKDQLAPGAQNVGTIEAISVETGRTAWKREQRAGQTSLMTTAGGL
                     LFGGDTAGRFRAYDAETGAVLWEVNLGSHVTGFPAAFAVNGKQYVAISTGATPNTFGL
                     ATLTPETRAGTANNLYVFALP"
BASE COUNT          539 a         1140 c         1180 g          626 t
ORIGIN      
        1 tcattcccga ggcgccgaac tcgaccgacg ctgcggcgaa cggtctgacg gctgccgtct
       61 gccgtcgtca acgctcgcca ccgccgtaaa gcgccgctgc tcgccggctc ggcgacgaac
      121 cgcgcaaggt cggcgaataa tgcggcggtg cgcgcgctgt cgaaagctgc cgtcgcgcgt
      181 tcgcgcgccg cggcgcgctc gcgccgcagg tagagctcgt agccgctcaa ttcgctgcgt
      241 tgctgggccg gcaacgttcg cgcgtaagcc ttgaaattgt ccgcgtacac gtcgaggtca
      301 cggacgtcgc cgagcgaagt tgcgaaccag cgcagatcgg cgccgaagcg cgcgacttcg
      361 tgccgcggca agaaacgcgc gaagagcttg aacgcgacgc gcagccgacg agccgctacg
      421 cgtaagcggt gaatgtcgtc gggagtcggc ggccgctcgc ccgccggccg actgtcggcc
      481 agcgcttgcg cctcgcgctg cagcgtcgca cgggcgaatg aaatgaaggc gtcggcggtg
      541 ccaccgccgg ccggcgcacg cggaagggaa tccgttggcg caacggttcg ttgtgcgcgt
      601 cgctcgttca tgcaaggaaa attgggcttg ctctgccccg atgtgcctat gacattagtg
      661 accgcggcta cggggtgtca aggcgcgctc gtcgccgtcc ccgcgtactc atgaacttgg
      721 ctaggaagca gaactaatct atggaaatta ttggcgtcta caacttcaaa ggcggcgtcg
      781 gcaagaccac gacagccgtc aatctcgcgt atcgctcggc ggccgaggat tggccgaccg
      841 ttctctggga cctcgacagc cagggcgccg cgacgtactg cttgcggcgc gacgcgcagg
      901 agactggcat cgccaaagag ctcgttcgcg gcgatgcggc tctgcgcgac gtcgtcgtcg
      961 cgaccgagca cgagcggctg gacctgattc ccgccgattt ctcttaccgg cgaatggacg
     1021 tgcatctcga caagcgccag gacgcgacga cgttgctgct gaagctcatg cgcccgctgc
     1081 aagcgcgcta cgcgtgcttg atactcgatt gcccgccggg aatgtcgctg gtcgccgaga
     1141 acgtcatgca cgccgccgac gcgctcgtcg tgccggtgct gccgacgccg ctgtcggccc
     1201 gaatgctcga gcagctgttc cagttcttcg aggcgcgcgg ctggcgcgac gtcaacgtcc
     1261 tgccgttctt ctcgatggtc gatcgacgtc gcgcgctgca caagcagacg atcgacgagc
     1321 tccgcgcacg cttcccgacg atgctcgaga ccgaggtgcc gtacggcagc gaattcgagc
     1381 gcatgacggc gcgccgcgca ccggtcgagt cgtacgcacc ggcgagcgcg gccgccgaga
     1441 tctatcgctc gctctggcgc gagatcgatg cgcggctctc ttcgctgtcg agcgcgcggg
     1501 ccgccaaggg caccccgaac gccttgccgg cgccggcgac ggaggccggc ggcgccgcta
     1561 ccgacgcgga cctcgtcggc agcccgtggt cgggcggccg ctagctgccg ggacctctta
     1621 cgggagggcg aatacgtaga ggttgttggc ggtgccggcg cgcgtctccg gggtcaacgt
     1681 cgcgagaccg aacgtattcg gcgtcgcgcc ggtgctgatg gcgacgtact gcttgccgtt
     1741 caccgcgaac gccgccggga aacccgtgac gtgcgagccc aagttcactt cccacaaaac
     1801 cgcgcccgtc tcggcgtcat aggcgcggaa gcgcccggct gtatcgccgc cgaacagtag
     1861 gccgccggcc gtcgtcatca acgacgtctg gccggcgcgc tgctcgcgct tccacgccgt
     1921 gcgccctgtc tcgacggaaa tcgcttcgat cgtgccgacg ttttgcgcgc ccggcgcgag
     1981 ctgatccttg gtccggatcg cgtagagctc gtcgaggctc gggcgggccg ccgtcgacgt
     2041 cgacagcatg cagacgtttt gcagcggcat gtacaggagc ttcgtatccg ggctatacgt
     2101 gcccgcgtga tagttcttgc cgcccgtcaa gctcgggcaa acgagaatct cctggttggg
     2161 gcccgtgaac attttctctt cgttcacgac gccttggccc gactccgtgt tcacgctctt
     2221 gatgacgttc tgctcgacgg tcggccgcgc ccagaggaat tgccccgtct cgcggtcgag
     2281 cgtgtagacg aggcccgtct tgccgggaac gcccgtcacg actttgtagc gctgacccgc
     2341 gcgcacgcgc ggattgatcc aagccacggc gttcgcatcc ggcgcgacga ccgtgtcgat
     2401 gaggatgcgc tcgaacgggt gatcgaggtc ccagtgatcg acgttgtgct gtaggtacca
     2461 aacgatcttg ccggtgtcgg cgtcgatcga caacgtgctg ttgtgataga ggtactgctt
     2521 gtcgttgccg gccagcatga acttcggcgc cggcgacgtc accgacgtgc ccacatagat
     2581 gcggttcagc tcggggtcga agctcggcac catccacaag ccgacgtgcc ggcgatcctc
     2641 gtacgggatg ttgccccacg tgtcgccgcc gggctcgtcg gggcgcggga tcgtgcgcgt
     2701 gcgccagact tccttgcccg tcagcgcgtc gaacgccgtc agcacgcagg cttccggtcc
     2761 gccctcgggc tcgcagctgc ggcccgagat gaccttgccg ttcgcgacga tcggcccgga
     2821 gctgtgctgc gcgccgtgct tgtagtcgag gatcttggtt tcccaggcca gcgcgcccgt
     2881 gcgcgcgtcg agcgcgtagg cgaagttgtc ggcgccgttg tcgaggatca agttgccgta
     2941 aatcgccaag ttgcggttga tcgacggaaa cgggatgtaa tcggcgttgt catcctgcac
     3001 gggacggcgg tattcccaga tgaggtcgcc cgtcgcggcg tcgatcgcct gcgtgatgtc
     3061 gttcgggttc gggaagaaca acacgccgtc gtgcacgagc ggcgtgcctt cctgcacgcc
     3121 ttgcgcgatc ggccgcgtcc acacgagctt cagctgcgcg acgttgcgct tgttgatctc
     3181 gttgagcggg ctatagcccc agtggttcag cgtgcggcgc cacgacagcc agtcggcggc
     3241 gtccggattc tgcagcatca cgcccgtcac aggacgcagc gtgcgttgtt gttgttgttg
     3301 tgcagtggcg gccgtgcagg cgagagcgcc gagcacggcg gcgcacacgg ccgtcatcgt
     3361 cgtgtttgca ggcaatcgca ttaaagctcc ggctcagttc aagcagtttg ggtcgcgtgg
     3421 tcgccggcga tcggctcggc ggtatgcccg acggagtagc ggtacttcgg catgaacgac
     3481 atcag
//
LOCUS       DATLEE010000035         6602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8216358, whole genome shotgun sequence.
ACCESSION   DATLEE010000035 DATLEE010000000
VERSION     DATLEE010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8216358"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VM692_00730"
     CDS             complement(<1..260)
                     /locus_tag="VM692_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HUQ50711.1"
                     /translation="MTRSSSEELRVLVLPPTTADGLAIQKLLAAGKIECRILRSVTEV
                     CLAIREGAGVVVISEEALLDDAEPLLACIGSQAVWSDLPVIV"
     gene            complement(276..1778)
                     /locus_tag="VM692_00735"
     CDS             complement(276..1778)
                     /locus_tag="VM692_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HUQ50712.1"
                     /translation="MKTGAAEPVAASGIAGLDDILRGGFPRECLFLIAGTPGTGKTTL
                     AMQFLLEGAKRGETCLYVTLSETRPEIEKVARSHGWDLSKVQMAELVPSEHNLSADSH
                     LTVFNPSEVELGETTEALIAAANTHRPQRLVVDSLSELRLIAQNPLRYRRQVLALKQF
                     FSNRECTVLMLDDRTGGLEDDHLQSIAHGVIVLEQLVNQYGAERRRLRVSKLRGVAFR
                     GGFHDFAIRRGGLDVFPRLVAAEHPADFVEHELPSGNAQLDRLLGGGLPAGTSTLLLG
                     PAGTGKSTVGTLFATAAAERGERAALFLFDENIGTFRSRSRKLGIPVDRGIDKGLITV
                     QQIDPAELSSGEFAAIARRAADGTDSNGKPAKVIVVDSLNGYLNAMPEEKFLTAQLHE
                     LLSFLGQRGVATILNVTQTGMVGSMGSPVDTTYLADNVILFRFFEANGWIRRAISVVK
                     KRNGYHERTIRELDMNERGLVIGEALEGFQGILTGVPTYTGQAGTLMEKR"
     gene            2049..3011
                     /locus_tag="VM692_00740"
     CDS             2049..3011
                     /locus_tag="VM692_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ50713.1"
                     /translation="MMQSIDEGAFVDLGGVPQWLTLRGADRGNPALLLVGGAGFGYAA
                     LAPFFADWERDFTLVQWDQRGAGFTFARSGVEVTTMAQLVADGIAVAEYACARLGARK
                     LALACFSGGTIVGLEMVKRRPGLFSAYVGNGQVVDWARQDTLSYERLLERARVLRDAS
                     MLQELTAIGAPPYADAATDAVKSKYAGAPTPREAAAFGELGPLVGAALQGLPAGATYL
                     APGLRWPEPRARSFAAYTALRGDIVSFDARRLGLDFTVPMFFLQGADDVYTVSSEVES
                     YAAELVAPHVEYVAIAGAGHAAMFLRHEVHALLQRHVRATLLRA"
     gene            3133..3918
                     /gene="murI"
                     /locus_tag="VM692_00745"
     CDS             3133..3918
                     /gene="murI"
                     /locus_tag="VM692_00745"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HUQ50714.1"
                     /translation="MRNANAIGVFDSGVGGLSVLRHVRAQLPRLPLLYVADSGHVPYG
                     DKSADYIRGRSLALTEFLLTQGAATVVIACNTATAAAAALLRSRFEIPIVAMEPAVKP
                     AVTATRSRVVGVLATVGTLESARFAALLEQYAGDVEIVTQACPGLVEQVEAGDLAGAR
                     TRELVERYVRPLLERRADTLVLGCTHYPFLRPLIAEIAGPDVKLIDTGEAVARQVVRR
                     LPEKLLGRMDATPDEHFWTTGDASTAARIVSQLWGRAVDVGAL"
     gene            complement(3924..4385)
                     /gene="trxA"
                     /locus_tag="VM692_00750"
     CDS             complement(3924..4385)
                     /gene="trxA"
                     /locus_tag="VM692_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HUQ50715.1"
                     /translation="MADVTSIRCLKCGATNRVPRAKLAQGLQPVCGRCKQALPAAAAA
                     PLTVTDATFAADVERSPLPVLVDAWAPWCGPCRMIAPALEELAAELDGQARVAKLNVD
                     ENPQTANRFDVRSIPTLLVIVGGREVDRIVGAQPKHAIRERFERAIANARR"
     gene            4575..6065
                     /locus_tag="VM692_00755"
     CDS             4575..6065
                     /locus_tag="VM692_00755"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HUQ50716.1"
                     /translation="MLASLVLAASGALNMPCAELKNIELERATITSAVDVAAGLFKPP
                     GNANPQQQQAGPPPQPIPQHCRLTLVLKPTPDSNINVELWLPTENWNGKFLAVGNGGW
                     AGSIQGYGDMQEALRRGYATAATDTGHSEADGPAGMFALGHPEKIVDFAYRALHDMTV
                     KSKRLIDVFYEEPLDYSYYKGCSTGGRQGVMAAQRYPGDYDGIIAGALANRHVHMHTA
                     GSYRSINLARHPDEALSEAKAQLVNDAVMKQCDTLHEGFLNNPRQCSFDFDTLACPAG
                     KTSDTCLTPGELKSVHTFYDGLHTKAGELVFSGQALGNPIPALVSRPEGPGDNLTDSI
                     RILGFQNAAYDWRQFDLDRDLPFIDAAAGFVDAVNPDLRAFEAHGGKLLLYAGWRDTG
                     ITPENTVFYYESVREEMGPAQDDWLRLFMVPGMGHCRGGPGVDTFDTLATLEQWREKD
                     AAPSVMPARNRETGMARPLCAYPSYAKYDGSGDLNDAGNWTCTAPQ"
     gene            complement(6136..>6602)
                     /locus_tag="VM692_00760"
     CDS             complement(6136..>6602)
                     /locus_tag="VM692_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50717.1"
                     /translation="GGDPEFVATAEKTLALDPRNPEMLGLLGILLAAYGDTVHATELI
                     DRAHALTGQPRPMFNLGYVFAYLQEDDGCAALPLALELDAPKWFIAPMVTAAAAGLCG
                     DREAADAARTRLLAIAPRFEAEFPALIEVWRFDPRLRDALRRGLRQAGFEMR"
BASE COUNT         1072 a         2215 c         2246 g         1069 t
ORIGIN      
        1 aaaacgatga cggggagatc ggaccaaacg gcttgcgagc cgatgcaagc gagcaacggc
       61 tccgcgtcgt cgagcaacgc ttcttcggag atgacgacga cgccggcccc ctcgcggatc
      121 gccaagcaca cttcggtgac gctgcgcagt atgcggcact cgatcttgcc ggctgcgagc
      181 agcttttgga tcgcgagtcc gtcggcagtg gtggggggca agaccagcac gcggagctct
      241 tcggacgatg agcgcgtcat gccggttgcc ggcatctagc gtttctccat gagagttccg
      301 gcttggccgg tgtaggtcgg cactccggtc agaatgccct gaaagccctc gagcgcctcc
      361 ccgatgacga ggccgcgctc gttcatgtcg agctcgcgga tcgttcgctc gtgatagccg
      421 ttgcgcttct tgacgacgga gatcgcgcgg cggatccagc cgttggcctc gaagaagcgg
      481 aacagtatga cgttgtccgc gaggtacgtg gtatcgaccg gcgagcccat gctaccgacc
      541 atgccggtct gcgtgacgtt gagaatggtc gcgacgccgc gctgaccgag gaaggacagc
      601 agctcgtgca gctgcgccgt caggaatttt tcctcgggca tcgcattcag atagccgttg
      661 aggctatcga cgacgatcac cttcgcgggc ttgccgttcg agtcggtgcc atcggcggcg
      721 cgacgcgcga tcgcggcgaa ctcaccggac gaaagctcgg cggggtcgat ctgttgaacg
      781 gtgatgagcc ctttgtcgat gccccgatcg acggggatgc cgagcttgcg cgagcgcgac
      841 ctgaaagtgc cgatgttctc gtcgaacagg aacagcgccg cacgctcgcc gcgctctgcc
      901 gcggccgttg cgaacagcgt gccgaccgtc gacttgccgg tgcccgccgg ccccagcagc
      961 aacgtgctgg taccggccgg tagtccgccg ccgagcaggc gatcgagctg cgcattgccg
     1021 ctcggcagct cgtgctcgac gaaatccgcc ggatgctcgg ccgcaaccaa gcgcgggaac
     1081 acgtcgagcc cgcctctgcg aatggcgaaa tcgtggaagc cgccgcgaaa cgctacgccg
     1141 cgcagcttgc tgacgcgcaa gcgtcgacgc tccgcaccgt actgattgac gagctgctcg
     1201 agcacgatga cgccgtgggc gatgctctgc aggtgatcgt cttcgagccc gcccgtacgg
     1261 tcgtcgagca tcagcacggt gcattcgcgg ttgctgaaga actgcttgag cgcgagcact
     1321 tggcggcggt aacgcagcgg gttctgcgcg atgagtcgaa gctccgacag cgagtcgacg
     1381 accaaccgct gtggccgatg cgtgttcgct gccgcgatca acgcctcggt ggtctcgccg
     1441 agctcgacct cggaaggatt gaacacggtc agatgcgaat cggccgagag gttgtgctcg
     1501 gacggcacga gctccgccat ctgcaccttc gaaagatccc agccgtggct gcgcgccact
     1561 ttttcgatct ccggcctcgt ctccgagagc gtgacgtaca ggcaggtttc gccgcgcttc
     1621 gcaccctcca gcaagaactg catggcgaga gtggtcttgc cggtccctgg cgttcccgcg
     1681 atcaaaaaca gacactcgcg cggaaagccg ccgcgcagaa tgtcgtcgag acccgcaatg
     1741 ccggaagctg caaccggttc ggccgcaccc gttttcatag cacctttccc aaattcgtcc
     1801 tgcgcagccg ggtgagccgg cgcccgcagc tgcaatgata cgggtcgggt ttcgctcgga
     1861 ccagcggggc ctgggccgtc gcggcgaggc gcgtatcccg cgctgtcgag ctgcgcgcgc
     1921 ttgacgctcg gtccgccgag cgctcatgat cgccacgctg ttcagcagca cacgcgaagg
     1981 cctccgccgc actccggcgg gggccttttt atttgtctcg ccgcacgtgc ggtgcaaacg
     2041 aggcaacgat gatgcaatcc atcgacgaag gcgcgttcgt agacttgggc ggtgttccgc
     2101 agtggctcac gctgcgcggc gccgatcgcg gcaatcccgc gctgctgctc gtcggcggcg
     2161 ccgggttcgg ctatgcggcg cttgcgccgt tcttcgcgga ctgggaacgc gacttcacgc
     2221 tcgtgcagtg ggatcagcgc ggggccggct ttacgttcgc gcgctccggt gtcgaggtga
     2281 cgacgatggc gcaactcgtc gccgacggca tcgccgtcgc ggaatacgcc tgcgcgcggc
     2341 tcggtgcgcg caagctcgcg ctcgcgtgtt tctcgggcgg cacgatcgtc ggcctcgaga
     2401 tggtcaagcg ccgccccggg ctgttctcgg cctacgtggg caacggccag gtcgtcgatt
     2461 gggcacgcca ggacacgctg agctacgagc ggctgctcga gcgcgcgcgc gtgctccgcg
     2521 acgcatcgat gctgcaagag ctcaccgcga tcggcgcgcc gccgtatgcc gacgccgcga
     2581 ccgatgccgt caagtcgaag tacgccggcg ccccaacgcc gcgcgaggcc gccgcgttcg
     2641 gcgagttggg tccgctcgtc ggcgccgcgc tgcaggggtt gccggccggc gcgacgtatc
     2701 tcgcgccggg actgcgatgg cccgagccgc gcgcgcgttc gttcgccgcg tacacggcat
     2761 tgcgcggcga catcgtgagc ttcgatgcgc gccgtctcgg gctcgacttc acggtgccga
     2821 tgttttttct gcagggtgca gacgacgtgt acacggtctc gtcggaagtg gagtcgtatg
     2881 cggcggagct cgtagcgccg cacgtcgagt acgtcgcgat cgcaggcgcg gggcacgccg
     2941 cgatgttcct gcggcacgag gtgcacgcgc tgctgcagcg ccacgtgcgc gcgacgctgc
     3001 tgcgcgcatg actgcagaac ggcccgcaag ttgctgagag ccccgcggtc ggactagagt
     3061 cgctgcgcga atcgacggtc acggcaaggt ctcgatggtc gaacaacccg ctcgaagagc
     3121 agctcggcgc cggtgcgcaa cgccaacgcg ataggcgtat tcgactccgg cgtcggcggc
     3181 ctctcggtgc tcaggcacgt ccgtgcgcag ctgccgcggc tgccgttgct gtacgtggcc
     3241 gactcgggcc acgtccctta tggcgacaag agcgccgact acatccgcgg gcgctcgctc
     3301 gccctgacgg agttcttgct gacgcaaggc gccgcgaccg tcgtcatcgc ttgcaacacg
     3361 gcgacggccg cagcggcggc actgctgcgc tcgcgtttcg agattccgat cgtcgcgatg
     3421 gagcccgccg tcaagcccgc cgtcacggcc acgcgcagcc gtgtcgtcgg cgtgctcgcc
     3481 accgtcggca cgctcgaaag cgcgcgcttc gcggcgctgc tcgaacagta cgccggcgac
     3541 gtcgagatcg tgacgcaggc gtgcccgggt ctcgtcgagc aggtcgaagc aggagacctc
     3601 gcaggcgcgc gcacgcgcga gctcgtcgag cgctacgtgc ggccgctgct cgagcggcgc
     3661 gctgacacgc tcgtgctcgg ctgcacccat tatccgttcc tgcgcccgct gatcgccgag
     3721 attgcgggcc ccgacgtgaa actcatcgac accggcgagg ccgtcgcccg tcaggtcgtg
     3781 cggcgcttgc ccgagaagct gctcgggcgc atggacgcga ctcccgacga gcatttttgg
     3841 acgaccggcg atgcgtccac cgccgctcga atcgtgagcc agctttgggg ccgcgccgtc
     3901 gacgtcggcg cgttgtagcg atgtcagcgg cgcgcgttcg cgatcgcgcg ctcgaaacgc
     3961 tcgcggatcg cgtgcttcgg ctgcgcgccg acgatgcgat cgacctctcg gccgccgacg
     4021 atgacgagca gcgtcgggat gctgcgcacg tcgaagcgat tggcagtctg cggattctcg
     4081 tcgacgttga gcttcgcaac gcgagcctgg ccgtcgagct ctgccgcaag ctcctcgagc
     4141 gccggcgcga tcatgcggca ggggccgcac cacggcgccc aggcatcgac gagcacgggc
     4201 agcggcgagc gttcgacatc ggcggcgaac gtcgcatcgg taaccgtgag cggcgcggcg
     4261 gcggccgccg gcagcgcttg cttgcagcgg ccgcaaacgg gttgcagccc ttgcgcgagc
     4321 ttcgcgcgcg ggacgcggtt ggtcgccccg cacttgaggc aacggatcga agtgacgtcg
     4381 gccatcggtg ttctcgaatc gccagcagaa gcttggcaga tgagggctcg cccacgcgac
     4441 gtcaagctgc gccgcgccgc atgatggcgc gctgacacta gcgccgattg cgtcgcgctt
     4501 ggaccgcatc gcgcggcggc gcatgatgga gcgtacggtc ataagccgta cgcaaccggc
     4561 ttcgaggggg aagcatgtta gcgagccttg tattagccgc atcgggtgca ttgaacatgc
     4621 cgtgtgcgga gctcaagaac atcgagctcg agcgcgcgac catcacgagc gccgtcgacg
     4681 tcgccgccgg gctgttcaag ccgccgggca atgcgaatcc gcagcagcaa caggccgggc
     4741 ccccgccgca gcccatcccg cagcactgtc ggctgacgct cgtgctgaag ccgacgccgg
     4801 attcgaacat caacgtcgag ctgtggctgc cgaccgagaa ctggaacggc aagttcctcg
     4861 ccgtcggcaa cggcggctgg gccggctcga tccagggtta cggcgacatg caggaggcgc
     4921 tgcgccgcgg ctacgcgacg gcggcgaccg acacgggcca ctccgaagcc gatggccccg
     4981 ccggcatgtt cgctctcggc caccccgaga agatcgtcga cttcgcgtat cgcgcgttgc
     5041 acgacatgac cgtgaagtcg aagcggctca tcgacgtgtt ctacgaagag ccgctcgact
     5101 actcctatta caaagggtgc tcgaccggcg gccggcaagg cgtcatggct gctcagcgct
     5161 accccggcga ctacgacggc atcatcgccg gcgcgctcgc gaatcggcac gtgcacatgc
     5221 acacggccgg ctcgtatcgc agcatcaatc tcgcgcgtca tcccgacgaa gcgctctcgg
     5281 aggcgaaggc gcagctcgtc aacgatgccg tgatgaagca atgcgacacg ctgcacgagg
     5341 ggttcttgaa caatccgcgc cagtgctcgt tcgacttcga cacgctcgcc tgtcccgccg
     5401 gcaaaacgtc cgacacctgc cttacgccgg gcgagctcaa gagcgtgcac acgttctatg
     5461 acggcttgca cacgaaagcc ggcgagctgg tcttctcggg gcaagcgctg ggcaacccga
     5521 tcccggcgct cgtcagccgg cccgagggcc ccggcgacaa cctgaccgat tcgatccgga
     5581 tcttggggtt ccagaacgcg gcctacgatt ggcggcagtt cgatctcgac cgcgacctgc
     5641 cgttcatcga cgcggcggcc gggttcgtcg acgccgttaa ccccgatctg cgcgcgttcg
     5701 aagcgcacgg cggcaaactg ttgctgtacg caggctggcg cgacaccggc atcacgcccg
     5761 agaacacggt cttctattac gagagcgtgc gcgaggagat gggccccgct caggacgact
     5821 ggctgcgcct gttcatggtg cccggcatgg gccattgccg cggcggtccc ggcgtcgaca
     5881 cgttcgacac gctggcgacg ctcgagcaat ggcgcgagaa ggatgccgcg ccgagcgtca
     5941 tgccggcgcg caatcgcgag acggggatgg cgcggccgtt gtgcgcctat ccgagctacg
     6001 cgaaatacga cggctcgggc gatctcaacg acgcgggcaa ctggacgtgc accgcgccgc
     6061 agtaatccca gacattgcag ccgactgggc caagtctccg cagagttgcg cgctcttact
     6121 tggcgtgagc cggcactagc gcatctcgaa gccggcttgg cgcagcccgc gccgtaacgc
     6181 gtcgcgcaag cgcggatcga agcgccagac ttcgataagc gcaggaaact cggcttcgaa
     6241 ccgcggcgcg atggcaagca gccgtgtgcg agccgcgtcg gcggcttcgc ggtcgccgca
     6301 cagtccggcc gcggcggccg tcaccatcgg agcgatgaac cacttcggcg catcgagctc
     6361 gagcgccagc ggcagtgcgg cgcagccatc gtcttcctgc aggtacgcga acacatagcc
     6421 caaattgaac atcggccgcg gctgccccgt cagcgcgtgg gcgcggtcga tgagctcggt
     6481 ggcatgaacc gtatcgccgt aggccgcgag caggatgccg agcagcccca gcatttcagg
     6541 gttgcgcgga tcgagcgcga gggtcttctc ggcggtcgcg acgaactcgg ggtcgccgcc
     6601 gt
//
LOCUS       DATLEE010000036         4431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2273902, whole genome shotgun sequence.
ACCESSION   DATLEE010000036 DATLEE010000000
VERSION     DATLEE010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2273902"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VM692_00765"
     CDS             complement(<1..215)
                     /locus_tag="VM692_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50718.1"
                     /translation="MIVARAPGKVVALGEYAVLDGAPAVVLAVDRFVEVTIAASPDGA
                     CRLVMKAGATTERVLVPGQPTGLPLLDL"
     gene            complement(216..1232)
                     /gene="mvaD"
                     /locus_tag="VM692_00770"
     CDS             complement(216..1232)
                     /gene="mvaD"
                     /locus_tag="VM692_00770"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HUQ50719.1"
                     /translation="MTTSKNPAAAVANPAGAAKARAGANFALIKYWGKADAQLNVPAV
                     GSISITLDRLYTETTVELASDAENDELTLNGARRDEDLPKISACLDLLRRKAGFTARV
                     RVTSRNNFPTGAGLASSASGFAALVRAAEAAFGLTLSPRERSIVARQGSGSAARSVFG
                     GFVEMHAGTAADGSDSFAEPLLDAADWPLEVVIAVTAKGEKEIGSRSGMTRSATSSPY
                     YAAWVAEQPPDLVAAKTAIRARDFAPLAELAEHNCLKMHAAALAARPPLVYWNGATVE
                     CLHAVRRLRAGGVPVFFTIDAGPQLKAVCEPGARGAVERALHDVPGVLELLTSALGPG
                     AELS"
     gene            complement(1219..2208)
                     /gene="hpnA"
                     /locus_tag="VM692_00775"
     CDS             complement(1219..2208)
                     /gene="hpnA"
                     /locus_tag="VM692_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HUQ50720.1"
                     /translation="MTPALVTGANGFLGSAVVRALLAEGAAVRALARPGSDRRNLAGL
                     DVEIVDGDLTDRGSLTRAAAGCAAVFHVAADYRLWVADSAPMYRANVEGSVNVLEAAA
                     AAGAARMVYTSSVAVLGINRDRTPADESTPVSVDDMVGHYKRSKFLAEQAVRKRAQEL
                     GFAVVTVNPSTPIGPRDIKPTPTGRILLDAAAGRMPAFVDTGLNLVHVDDVAQGHVAA
                     WRVGRPGERYVLGGDDFTLQRILELVAEQVGRRASTIRLPHWSVYPLAVGAEALAKVT
                     KREPRVTLDGVRMSTKHMYFSSRKAQQELGYKWRDPRLAIAAAVDWFKTNDYF"
     gene            complement(2205..>4431)
                     /locus_tag="VM692_00780"
     CDS             complement(2205..>4431)
                     /locus_tag="VM692_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HUQ50721.1"
                     /translation="LYLPTTELAALTDRLAAAQPLIGLLQARFDGAAVLDVARQTVTA
                     ANGADAALDSFYEELARTIDGAADGAAVPLAWNRLIALGAPPTTRRIISLQPELDFSR
                     MQPAAAAIADIRAIAVRLNAERTEPVELRLTGSVAMEHEELMSVSRGAGIGALATLVM
                     VALVLYASLRSWRLLAVSLVTLLIGLALTAAFAGVAVGQLNLLSVAFVVLNVGLGSDY
                     VIHVLLRYRELTAAGASTQAALVETVRDVGSSLVLCAVTTAAGFYSFIPTTFSGVSEL
                     GLIAGTGVFFGLFASVTLLPALVAWWDADARPGSSAATWLDPRIFAPLSRRPRLVLGA
                     TAVTLAAALAVLPQVTFDSNPIHLRDPGSESVTTLLELAAAGEAPLLNLAAVAPDRAT
                     AVGWATALRMLPEVRSVTTTEQLVPGEQSAKLALLEDLELLMGPNFADLERAPRDDTA
                     LATALTELELASAAQSAARALHDAVARLRARLASAGGAERDATLARLDDSLTVGLPRQ
                     LARLSTALGAQPFERADLPPALAARWLASGGRELVEITAAEDVSDNAAARRFIDAVHA
                     IVPTATGLPVVYQEASATVVAAFERALLYAFVMVSAIIWLVLRDVKDTLLVLLPIALA
                     SILTAALTLVVGMPFNYANIIALPLLVGIGVDNGIHVVHRMRSDDAERLFDTSTMRAI
                     LASGLTTVASFGNLAFSAHVGTASMGILLAIGLAASMAATLIVLPAWLKLYPPRARAE
                     A"
BASE COUNT          731 a         1556 c         1547 g          597 t
ORIGIN      
        1 aggtcgagca gcggcaggcc ggtcggctgg cccgggacca acacgcgctc cgtcgttgcg
       61 cccgccttca tgaccaggcg gcacgcgcca tcggggctcg ccgcgatcgt gacttcgacg
      121 aatcggtcga cggcgagcac gacggccggc gcaccatcga gcacggcgta ctcgccgagc
      181 gcgacaacct tgcccggcgc gcgcgcaacg atcactcagc tgagctccgc accgggacct
      241 aaggcgctcg tcagcagctc gagcacgccc ggcacgtcat gcaacgcgcg ctcgacggcg
      301 cctcgcgcgc cgggctcgca gacggccttc agctgcggtc ctgcatcgat cgtgaagaac
      361 accggcacgc cgccggctcg caggcgccga accgcgtgca agcattcgac ggttgcgcca
      421 ttccaataaa cgagcggcgg ccgggccgcg agcgccgccg cgtgcatctt caagcaattg
      481 tgctcggcga gctccgcgag cggcgcgaaa tcgcgcgcgc ggatcgcggt cttggccgcc
      541 acgagatcgg gcggctgctc ggcgacccaa gccgcgtagt aaggcgacga ggtcgcggag
      601 cgcgtcatgc cgctgcgcga gccgatctcc ttttcgcctt tggccgtgac ggcaatgaca
      661 acctcgagcg gccagtccgc ggcgtcgagc agcggctcgg cgaagctgtc gctgccgtcg
      721 gccgcggtgc cggcgtgcat ctcgacgaag ccgccgaaga ccgagcgcgc ggcggagccg
      781 gacccttgcc gcgcgacgat gctgcgctcg cgcgggctca gcgtgaggcc gaaagcggcc
      841 tcggcggcgc ggacgagcgc cgcgaatccc gaggccgaag acgcgagccc ggcgcccgtc
      901 ggaaagttgt tgcggctcgt cacgcgaacg cgcgccgtga aaccggcttt gcgccgcagc
      961 aagtcgaggc acgccgagat cttcggcaag tcctcgtcgc ggcgcgcgcc gttcagcgtc
     1021 agctcgtcgt tttcggcgtc cgacgcgagc tcgacggtcg tctcggtgta caagcggtcg
     1081 agcgtgatcg agatcgagcc gacggccggc acgttcaatt gcgcgtcggc cttgccccag
     1141 tacttgatca gcgcgaagtt ggcgcccgcg cgcgctttag ccgctcccgc agggttcgca
     1201 accgctgccg ccgggttctt agaagtagtc attcgtcttg aaccaatcga ccgccgccgc
     1261 gatcgccagg cgcgggtcgc gccacttgta gccgagctcc tgctgcgctt tgcgactcga
     1321 aaaatacatg tgcttcgtcg acatccgcac gccgtcgagc gtcacgcgcg gctcgcgctt
     1381 cgtcacctta gcgagcgcct cggcgccgac cgcgagcgga tagacggacc aatgcggcag
     1441 gcgaatcgta ctcgctcgcc tgccgacctg ctctgcgacg agctcgagta tccgctgcag
     1501 cgtgaagtcg tcgccgccca agacgtagcg ctcgccgggc ctgccgacgc gccatgcggc
     1561 gacgtgcccc tgcgcgacgt cgtcgacgtg cacgagattc agtcccgtgt cgacgaaggc
     1621 cggcatgcgc ccggccgcgg cgtcgagcag gatgcggccc gtcggcgtcg gtttgatgtc
     1681 gcgcgggccg atcggcgtcg aaggattgac cgtgacgacg gcgaagccaa gctcctgcgc
     1741 gcgtttgcgg acggcttgct cggccaggaa cttcgagcgc ttgtagtgcc cgaccatgtc
     1801 gtcgacggac accggagtgc tctcgtcggc tggcgtgcga tcgcgattga tgccgagcac
     1861 ggccacgctc gacgtgtaga ccatgcgcgc cgcgcccgcg gcggcggccg cttcgagcac
     1921 gttgacggag ccttcgacgt tcgcgcgata catcggggcc gaatcggcga cccagagccg
     1981 atagtcggcc gcgacgtgga acacggcggc gcaaccggcg gcagccctgg tcaacgagcc
     2041 gcgatccgtc agatcgccgt cgacgatctc gacgtcgagc cctgcgaggt tgcgccggtc
     2101 gctgccgggg cgcgccagtg cacgtacggc agcgccttcg gcgagcaacg cgcgtacgac
     2161 ggccgagccc agaaagccgt tcgcgcctgt gacgagcgcc ggcgtcaagc ctcggccctc
     2221 gcacgcggcg ggtacaactt caaccacgcg ggcaggacga ttagcgtggc cgccatgctg
     2281 gccgccaagc cgatcgcgag cagaatgccc atgctcgcgg tcccgacgtg cgccgagaac
     2341 gcgaggttgc cgaagctcgc gacggtcgtc agaccgctgg ccaagatcgc gcgcatcgtg
     2401 ctcgtgtcga acaaccgttc cgcatcgtcg ctgcgcatgc gatgcacgac gtgaatgccg
     2461 ttgtcgacgc cgatgccgac gagcagcggc aacgcgatga tgttggcata gttgaacggc
     2521 atgccgacga ccagcgtcaa cgcggccgtc agaatcgaag ccagtgcgat cggcaggagc
     2581 acgagcagcg tgtccttcac gtcgcgcagc acgagccaga tgattgcgga caccatgacg
     2641 aacgcgtaga gcaatgcacg ctcgaacgcg gccacgaccg tcgccgacgc ctcttgatag
     2701 acgacgggca agcccgtcgc cgtcggcacg atcgcgtgca cggcgtcgat gaagcgccgc
     2761 gccgccgcgt tgtcgctgac gtcctcggcg gccgtgatct cgacgagctc gcggccgccg
     2821 ctcgcgagcc aacgcgcggc gagcgccggc ggtagatccg cgcgctcgaa cggttgcgct
     2881 ccgagcgcgg tcgagagacg cgcgagctgc cgcggcagcc ccaccgtcaa cgaatcgtcg
     2941 agccgcgcca acgtcgcgtc gcgctccgca cctccggcgg acgcgagccg cgcgcgcaaa
     3001 cgcgcaacgg cgtcgtgcag cgcgcgagca gcggattgtg cagcactcgc gagctcgagc
     3061 tccgtaagcg cggttgcgag cgcggtgtcg tcgcgcggcg cccgctcgag gtccgcgaag
     3121 ttcggcccca tcagcagctc gaggtcctcg agcaacgcaa gctttgcgct ttgctccccg
     3181 ggaacgagct gctccgtcgt cgtgacgctg cggacctcgg gcagcatgcg cagcgccgtc
     3241 gcccagccga cagccgtcgc gcgatcgggc gcgacggcgg cgagattcag caatggcgcc
     3301 tcgcccgcgg cggcgagctc gagcaacgtc gtgacggatt cgctcccggg atcgcgtaag
     3361 tggatcgggt tgctgtcgaa agtgacctgc ggcaacacgg ccaacgcggc ggcgagcgtg
     3421 acggccgtcg cgccaagcac gagccgcggg cgccgagaga gcggcgcgaa gatgcgcgga
     3481 tcgagccacg tcgctgcgct gctcccgggc cgtgcgtcgg cgtcccacca cgcgacgagc
     3541 gcgggcagca gcgtcacgct tgcgaacaag ccgaagaaca cgccggtgcc cgcgatcaag
     3601 ccgagctccg agacgcccga gaacgtcgtc ggaatgaacg aatagaaccc ggccgccgtc
     3661 gtcacggcgc acagcacgag cgaagagccg acgtcgcgca cggtctcgac gagcgcggcc
     3721 tgcgtcgacg cccccgcggc agtgagctcg cggtaacgca gcaacacgtg aatcacgtag
     3781 tcgctgccga ggccgacgtt caaaacgacg aacgcgaccg acaaaaggtt gagctggccc
     3841 acggcgacgc ccgcgaacgc ggccgtcagc gccaacccga tcagcagcgt cacgagcgac
     3901 acggcgagca gccgccacga gcgcagcgat gcatagagca cgagcgcaac catgacgagc
     3961 gttgcgagcg ccccgattcc ggcgccgcgg ctcacgctca tgagctcctc gtgctccatg
     4021 gcgacgctgc ccgtcaggcg cagctcgacg ggctcggtgc gctcggcatt gaggcgcacg
     4081 gcgatggcac ggatgtccgc gatcgcggcc gccgccggtt gcatgcggct gaagtcgagc
     4141 tccggttgca gcgagatgat gcgccgcgtc gtcggcggcg cgccgagcgc gatgagccgg
     4201 ttccacgcga gcggcacggc ggcgccgtcg gccgcaccgt cgatcgtgcg cgcgagctcc
     4261 tcataaaagg agtcgagcgc ggcgtcggcg ccgttggcgg cggtcacggt ttgacgcgcg
     4321 acgtcgagca cggcggcgcc gtcgaaccga gcctgcagca ggccgatcaa cggctgcgcc
     4381 gcggcgagcc tgtcggtcag agcagcgagc tccgtggtcg gcagatacaa a
//
LOCUS       DATLEE010000037         7465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2274785, whole genome shotgun sequence.
ACCESSION   DATLEE010000037 DATLEE010000000
VERSION     DATLEE010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274785"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..608)
                     /locus_tag="VM692_00785"
     CDS             complement(246..608)
                     /locus_tag="VM692_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUQ50722.1"
                     /translation="MKCTLAAFALVVWSGVALAQEPASAERGKAKFEHTCAPCHGEGV
                     GDDGRAMLPGTDALRIKYQGSLPALIEERTDLNAEAIRTFVRRGTWSMPPFRPTEVTE
                     REIQDIAAYLRQSSGAGR"
     gene            complement(605..2263)
                     /locus_tag="VM692_00790"
     CDS             complement(605..2263)
                     /locus_tag="VM692_00790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUQ50723.1"
                     /translation="MAILPPGVSAESFQSAIREFEAAIGREWVFTSDDDLYSYRDHFS
                     YIKDQPNELIPSAAVGPDTTEQVQAIVRTANKYKIPLYAISTGKNFAYGGPSPNVRGS
                     VTVDLKRMNRILVCDDKRHFALVEPGVSYIDLYRHIQQNKLNVWVDTPDPGWGSLIGN
                     AMDHGIGYTMGFYRDHFGAHTGMEVVLPNGEVMRTGMGALPASNSWQEYRHGYGPDPC
                     GLFGQGGFGIVTKMGFRLMPAPEHWRNGLVTVPKREDLIPLIEVVNYLGDLSMCGEPW
                     YGSPLTALMRNTDFREAALAFNEGEMDRLAASAKLHSWQVELQFYGSERTTLANWEYA
                     KELVARKVPGARFTDGESLKTPLTREQIAATTGPYPTNMRRNITQGVPGLGIWYMTGR
                     TEQNPNAWNETHIGLFSLIGRSGEAVLKAQKDFADVARRLEMPSLFPNAIQTPVNWYQ
                     FAFLMGNGFGLGGGDVSPAGKLKATAKLREVLKENAKLGYGDYRAPPILQDDVADQYS
                     FNDFALRRFVETMKDAVDPNGILSPGRAGVWPAPFRSMRGGLRS"
     gene            complement(2273..2878)
                     /locus_tag="VM692_00795"
     CDS             complement(2273..2878)
                     /locus_tag="VM692_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50724.1"
                     /translation="MLNRRDVLRTAAVLPAWALTALPFARAAAASAGVLTLERFIFDV
                     RFAEAGAIAAQVEQRGVELAPIADDLMTLWYDDLDLAWREAPMALAGVTLVEALFVLE
                     TFALDRGMHVVFRGEHGLVENGKVTHRFAGPAPVVQQLSPLPRQWKAAVAKALTECPL
                     GAPEIVRTQAVSDGAALSLRDMPLVSWIIAPRAAVASKVVS"
     gene            3053..3532
                     /locus_tag="VM692_00800"
     CDS             3053..3532
                     /locus_tag="VM692_00800"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ50725.1"
                     /translation="MKKTFAVWAAGLGFASSLQAHHNSGLFETTTPIRVQGVIAEVRF
                     TNPHSMIFVDAVDLDGTHVRWAVENSGTLAMTRDRGFNETTVQVGDPIEACGYAPKRA
                     YQTAGELAAKGLPRPQPWWGNWDKVITGRLLILKGGPAEHWSHYGPLEACQALLESQ"
     gene            complement(3545..4414)
                     /locus_tag="VM692_00805"
     CDS             complement(3545..4414)
                     /locus_tag="VM692_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUQ50726.1"
                     /translation="MKTRPTSVAADADNRSRLLHASADVFAEFGFEGASLRAIADAAG
                     VSFQLIAYYFGSKEELWVATVDYLFERYLETGKGLGFTPSGNLNEQFHNHLRLLLTDM
                     MQRPQLHKICVQEHLSSSPRYRNIIAPKLEHMFTTLAMPYFREVVRLGIVERFTPEEV
                     CFLFSSICKANTAATDFVEQSLGVPARSVKAVEQQIDLIFAILTERTASIAEVEETPQ
                     RAPQSKAATRSPAATVATLPLGKLAAGQEERVEQLETENRNLKQLIGNLSLEKKLLLD
                     RLAELESSAAKKR"
     gene            4577..6988
                     /locus_tag="VM692_00810"
     CDS             4577..6988
                     /locus_tag="VM692_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ50727.1"
                     /translation="MRRHWLNAAIRAAIASQLAAAGLAQAQQGGLEEIVVTATRRETN
                     LQDVPISVIAITGDDLERRGVDSVERLSTSVPNLNMIGGLSGPGASSFTVRGIPRVGT
                     YIDGVWQVGTAGLLTRNLVDLERVEVLRGPQGTLYGRDSTGGAIRLVTKRPAEEFGAQ
                     LGATIGSLDRRDLNLSVDLPLGEKLRTKWTAASLERNGYIQSLTVDQDYGRIDDEVLR
                     GDVLWLPTEKLSFRFNYQSNEQSPTEARIQAAVFPELSQTFGIGAAPGAVRLAAGIIQ
                     LYTLAGQPINALTQQAGFPGGQVGKWQTRSEITVPDRFEDEQLSVDVQWSLSDYLSLQ
                     FLTARMNQYSNSYVDWDNTQYNVFNDIFANDLDLISQEIQISGDGERIEWVAGAYYWE
                     QENVSRNPSYSMGEFNNGQLNINNVFATPTCTNVPVGFLPCQATFGIIMGQQADDQNL
                     ARQRGWAVFGETVIHLTDALDLTVGYRHHDQTNQSSALAPIPGVTAAKPPRSNMAFGP
                     GDIFAAQRVGLLDSASFDKGTSRLAVNYEFTDDIMGYVGYSEGFNSGGFGVEQLTCKR
                     RVSPFEPEELKNYELGLRSDLRDGELRFNATLFHTKWDSIQLAGESIDDCFTPPRVGT
                     NLRTQNVAAAEAEGLEFELTIAATDNLLFNVNLGLLDTQYVGIATPVPGLTLNTEFSQ
                     APKTTGNVGVQHTANLRSGATFVTRVDYSHSDQFWRSQIPNFRTEYYGLPGQFDEGGD
                     YGLVNARLTYTPMAANWELSVFGTNLTNEYYLNSGFFHSLWDIDFATVGRPREAGVSL
                     KLMFD"
     gene            7087..>7465
                     /locus_tag="VM692_00815"
     CDS             7087..>7465
                     /locus_tag="VM692_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HUQ50728.1"
                     /translation="MLLGVGAAVLYGCAAPNPDLGALAPAEIDYNWHVRPILSENCFK
                     CHGPDPSSRKAGLRLDVSELAMAELPETPGKFAIVPGNIDRSELVSRIRATNVDDRMP
                     PESTHKTLSAQQMAILEQWIANGA"
BASE COUNT         1406 a         2456 c         2309 g         1294 t
ORIGIN      
        1 gggcgcgagc gctcgatacc gctcgtcgat cgccgcgctg cgcgaggccg ctacgaggcc
       61 ttgctgcaag gtcgtaattg tcgccacagg tgagccgacg tcggccacgg cgccgtaagg
      121 cgcaagcgcc gtcgcggcca gcagcatgaa gctcgttagg aaaaagcggc gatccatgca
      181 accaattcta gccgttgttc ctgaacggct gcgtaacacc gcgctttggc ctcctcggcg
      241 ccgctctagc gcccagcccc agacgactgc cgtaggtagg cggcgatgtc ctggatctcg
      301 cgctccgtaa cctcggtcgg ccgaaacggc ggcatcgacc acgtgccgcg gcgcacgaac
      361 gtacggatgg cttcggcatt gaggtcggta cgctcttcga tcagcgccgg cagcgagcct
      421 tggtacttga tgcgcaacgc gtcggtgccc ggcagcatcg cgcggccgtc gtcgccgacc
      481 ccttcgccat gacacggcgc gcacgtgtgc tcgaactttg ctttgcctcg ctcggccgaa
      541 gcaggctctt gcgcaagcgc aacgccggac caaaccacga gcgcgaacgc tgccaacgtg
      601 cacttcatga ccggagccct ccgcgcatcg agcggaacgg cgcgggccag acgccggcgc
      661 gccccggcga cagaatgccg ttcgggtcga ccgcatcctt catcgtttcg acgaaccggc
      721 gcagcgcgaa gtcgttgaac gagtactgat cggcgacgtc gtcctgcaag atcggcggcg
      781 cgcgatagtc gccgtagccg agcttcgcgt tctccttcag cacctcgcgc agcttcgccg
      841 tagctttcaa cttgcccgcg gggctcacgt cgccgccgcc gagcccaaag ccgttgccca
      901 tcaggaacgc gaactgatac cagttgacgg gcgtctgaat cgcgttcggg aacaggctcg
      961 gcatctcgag ccgccgcgcg acgtcggcaa aatctttctg cgcctttagc accgcctcgc
     1021 cgctgcggcc gatcagcgag aacaagccga tgtgcgtctc gttccaagcg ttcgggttct
     1081 gctccgtgcg gcccgtcatg taccagatgc cgaggcccgg cacgccttgc gtgatgttgc
     1141 ggcgcatgtt ggtcggatac gggcccgtcg tcgccgcgat ctgctcgcga gtcagcggcg
     1201 tcttcaacga ctcgccgtcc gtgaagcgcg cgcccggcac tttgcgcgcg acgagctcct
     1261 tcgcgtattc ccagttcgcg agcgtcgtgc gctcggagcc gtagaactgc agctcgacct
     1321 gccacgagtg caacttcgcc gacgccgcga gcctgtccat ctcgccctcg ttgaaagcga
     1381 gcgccgcttc gcgaaagtcc gtgttgcgca tcagcgccgt cagcgggctg ccgtaccagg
     1441 gctcgccgca catcgacaga tcgccgaggt agttgacgac ctcgatcaac ggaatcaaat
     1501 cttcgcgctt cggcacggtc acgagcccgt tgcgccagtg ctcgggcgcc ggcatcaagc
     1561 ggaagcccat cttcgtcacg atgccgaagc cgccttgacc gaagaggccg cacgggtcgg
     1621 ggccgtagcc gtggcgatac tcctgccagc tattcgacgc aggcagcgcg cccatgcccg
     1681 tacgcatgac ctcgccgttc ggcagcacga cctccatgcc cgtgtgcgcg ccgaaatgat
     1741 cgcgatagaa ccccatcgtg tagccgatgc cgtgatccat cgcattgccg atcaggctgc
     1801 cccagccggg atccggcgtg tcgacccaaa cgttgagctt gttctgctgg atgtggcgat
     1861 agagatcgat gtacgagacg ccgggctcga cgagcgcgaa gtggcgcttg tcgtcgcaga
     1921 cgagaatgcg gttcatgcgc ttcaagtcga ccgtgacgct gccgcgcacg ttcggggacg
     1981 ggccgccgta agcaaagttc ttgcccgtcg aaatcgcgta cagcggaatc ttgtacttgt
     2041 tcgccgtgcg gacgatcgcc tgcacttgct ccgtcgtgtc ggggccgacg gccgccgacg
     2101 gaatcagctc gttgggctgg tccttgatgt aggaaaagtg atcgcgatac gaatagagat
     2161 cgtcgtcgct cgtgaacacc cactcgcgcc cgatcgccgc ttcgaactcg cggatcgcgg
     2221 actggaacga ctcggcactg acgcccgggg gcaaaatcgc catacgtttt cctcaactca
     2281 ctactttgga cgcgaccgcc gctcgcggcg cgatgatcca cgacacgagc ggcatgtcgc
     2341 gcagcgacag cgcggcaccg tcgctgacgg cctgcgtgcg cacgatttcg ggtgcgccga
     2401 gggggcactc cgtcagcgcc ttcgcgactg cggctttcca ctgccgcggc agcggcgaca
     2461 gctgttgcac gaccggcgcc gggcccgcga agcgatgcgt gaccttaccg ttctcgacga
     2521 gcccatgctc gccgcggaac acgacgtgca tgccgcgatc gagcgcgaac gtctcgagca
     2581 cgaatagcgc ctccacgagc gtgacgccgg cgagcgccat cggcgcttca cgccaggcga
     2641 gatcgaggtc gtcgtaccag agcgtcatga gatcgtccgc gatcggcgcc agctcgacgc
     2701 cgcgctgctc gacttgcgcg gcgattgcac cggcctcggc gaatcgcacg tcgaagatga
     2761 agcgctcgag cgtgagcacg ccagcggaag ctgcagccgc acgcgcgaac ggcagcgccg
     2821 tcaaagccca ggcgggcagc acggcagccg tccgcaagac gtctctgcga ttgagcattg
     2881 tgctttccct cgcccgagtc ccaggctcga ggcctgtgcc caagcataaa cgactgacgg
     2941 atcagaattc gagtcgagct tccgagccgt tcgggcaccg cgttgacatc tctacgtctg
     3001 tagcttatct atatctacgg acgtagagat caaaagcctc gcggagattc cgatgaaaaa
     3061 gacgttcgcg gtctgggcag cgggactcgg tttcgcgagc tcgctgcagg cccatcacaa
     3121 ctccggactg ttcgagacca cgacgcccat ccgcgtgcaa ggcgtcatcg ccgaggtgcg
     3181 tttcaccaat cctcactcga tgatctttgt cgatgccgtg gatttggacg gtacgcacgt
     3241 gcgctgggcc gtagagaatt ccggcaccct agccatgaca cgcgaccgcg gcttcaacga
     3301 gacgacggtg caggtgggcg accccatcga agcgtgcggc tacgctccaa agcgcgccta
     3361 tcaaaccgcg ggagaattgg ccgcaaaagg ccttccacgc ccccagcctt ggtggggcaa
     3421 ttgggacaaa gtcatcaccg gccggctgct gatcctcaaa ggcggtcccg ccgagcactg
     3481 gtcgcactat ggtccgttgg aggcctgtca ggctctgctc gaatcgcagt gatgcggtag
     3541 ttatttaccg cttcttggcc gccgacgact cgagctcggc gagccggtcg agcaacagct
     3601 ttttctcgag cgataagttg ccgatcagct gcttcaggtt tcgattctct gtttcgagct
     3661 gctctacgcg ctcctcctga ccggccgcga gcttacccag cggcagcgtc gcaacggtgg
     3721 ccgcgggact gcgcgtcgcc gccttcgact gcggcgcgcg ctgcggcgtc tcctcgactt
     3781 ccgcgattga agcggtccgc tccgtgagaa tcgcgaagat caaatcgatc tgctgctcga
     3841 cggctttcac ggagcgcgcc ggcaccccaa gtgactgctc gacgaagtcc gtcgctgccg
     3901 tgttcgcttt acagatcgag gagaacaaga agcagacttc ctcgggggta aatcgctcca
     3961 cgatgccgag acgcacgacc tcgcgaaaat acggcatggc gagcgtcgtg aacatgtgct
     4021 cgagcttcgg cgcaatgatg ttgcgatagc gcgggctgct cgaaaggtgc tcttgcacac
     4081 aaatcttgtg cagctgcggc cgctgcatca tgtccgtcag cagcaagcgc aagtgattgt
     4141 gaaactgctc gttgagattg ccggacggcg tgaaccccaa gcctttgccc gtctcgagat
     4201 agcgctcgaa caaatagtcg accgtcgcca cccacagctc ttccttcgag ccgaagtagt
     4261 aggcgatcaa ctgaaacgac acgccggcgg catcggcgat tgcacgcaga gacgcacctt
     4321 cgaacccgaa ctcggcgaac acatcggccg atgcgtgcag caaacgcgac cggttatccg
     4381 cgtcggctgc aacgctcgtt gggcgggttt tcacaagcgc gagcgtagca gcctctactc
     4441 cgtctgacat cgggactctc tagcgcggcc agcttaattg acgactgtac aacacaacgg
     4501 tacattcgtc atagatggac gacaaaccgc atggacgcgg gcctgttccg acaacagacc
     4561 attgggggga aggacgatga gacgacattg gctgaacgcg gccattcgag ccgcgatcgc
     4621 gagccagctc gccgcggcgg ggctcgcaca agcacaacaa ggcggccttg aagaaatcgt
     4681 cgtcacggcg acacgccgcg agaccaatct gcaagacgtg cccatctcgg tgatcgcaat
     4741 caccggcgac gacctcgaaa ggcgcggagt cgattccgtc gagcgcttga gcacctcggt
     4801 gcccaacctc aacatgatcg gtgggctcag cggcccaggc gcgagctcgt tcaccgtccg
     4861 cggcatcccg cgcgtcggaa cttacatcga cggcgtgtgg caagtcggga cggccggtct
     4921 actaacgcgc aacctcgtcg atctcgagcg cgtggaagtg ttacgcggac cgcaggggac
     4981 cctctacggc cgcgactcga cgggcggcgc cattcgactc gtcacgaagc gccccgccga
     5041 ggagttcggc gcgcagctcg gtgccacgat cggcagcctc gatcggcgcg acctgaatct
     5101 gtcggtcgac ttgccgctcg gcgagaagct gcgcacgaag tggacggcgg cgagcctcga
     5161 gcgcaacggc tacatccaaa gcctgaccgt cgatcaagac tacgggcgta tcgacgacga
     5221 ggtgctacgc ggcgacgtgc tatggctgcc aaccgagaag ctgtcgtttc gcttcaacta
     5281 ccagagcaac gagcagtcgc cgacggaagc gcgcatccaa gccgctgtct tcccggagct
     5341 gtcgcagacc ttcggcatcg gcgcagcgcc gggagcggtg cggctcgccg ccggaatcat
     5401 ccagctctac acactcgccg gccaaccgat caatgcgctg acacaacaag cgggcttccc
     5461 gggaggccag gtcggcaagt ggcaaacgcg ttccgaaatc acggtgccgg atcgcttcga
     5521 ggacgaacag ctatccgtcg atgtccagtg gtcgctctcg gattatctga gcctgcagtt
     5581 cctgacggcg cgcatgaacc agtactcgaa cagctacgtc gattgggaca acacgcaata
     5641 caacgtcttc aacgacatct tcgcgaacga tctcgatctc atcagccaag agatccaaat
     5701 ttccggcgac ggtgagcgca tcgagtgggt cgcgggcgcg tactactggg agcaggaaaa
     5761 cgtcagccgc aatccgagct acagcatggg ggagttcaat aacggccagc tcaacatcaa
     5821 caacgtgttc gcgacgccca cgtgcacgaa cgtgcccgtc ggctttctgc cttgccaggc
     5881 gacattcggc atcatcatgg gccagcaagc ggacgatcag aacctggcgc ggcaacgcgg
     5941 ctgggcggtg ttcggcgaga ccgtgattca cctgaccgac gcgctcgacc tcacggtcgg
     6001 ttatcgccat cacgatcaga cgaatcagag ctcggctctc gcaccgatcc cgggggtcac
     6061 ggccgcgaaa ccgccgcgct cgaacatggc tttcggtccc ggcgatatct tcgccgctca
     6121 gcgcgttgga ttgctcgact cggcatcatt cgacaaaggc acgagccggc ttgccgtgaa
     6181 ctacgagttc accgacgaca tcatggggta tgtcggctat tcggagggct tcaactctgg
     6241 cggctttggc gttgagcagc tcacgtgcaa gcgccgggtc tcgccgttcg agcccgagga
     6301 gctcaagaac tacgagctcg gcctgcgctc ggatttgcgc gacggcgagc tccgcttcaa
     6361 cgcgacacta ttccacacga agtgggacag cattcagctt gccggcgagt cgatcgacga
     6421 ttgcttcact ccgccgcgcg tgggcacgaa cttacgtacg caaaacgtcg cggccgcgga
     6481 agccgagggc ctcgagttcg agctgacgat cgcagcgacg gacaaccttc tgttcaacgt
     6541 caatctcggc ctgctcgaca cacaatacgt cggcatcgcg acgcccgtac cggggctgac
     6601 gctgaacacg gagttcagcc aggccccgaa gacgacgggc aacgtcggcg tgcagcacac
     6661 ggcgaacctg cgcagcggcg cgacgttcgt tacgcgcgtc gactacagcc attccgatca
     6721 gttctggcgt tcgcagatcc cgaacttccg caccgagtac tacggactcc ccggccagtt
     6781 cgatgaaggc ggcgattacg ggctcgtcaa cgcacgcttg acgtatacgc cgatggccgc
     6841 gaactgggag ctgtcggtgt ttgggacgaa tctgaccaac gagtactatc tgaactccgg
     6901 ctttttccat tcgctgtggg acatcgactt cgccacggta ggccggccgc gcgaagccgg
     6961 ggtgtctctg aaactgatgt tcgattaggg gtggacggcg tgacgactgc gcgattggta
     7021 accggcgaca agccctcgcg tccccgcgcg aggcgccgac ctgagccccg cgcattcgcg
     7081 cgcagtgtgc tcctcggcgt tggcgcagcc gtgctctacg gctgcgccgc gccgaatccc
     7141 gacttgggcg cgctcgcgcc cgctgaaatc gactacaact ggcacgtccg gccgatcctg
     7201 tcggagaact gcttcaagtg tcacggcccg gatccgtcgt cgcgcaaggc gggtctgcgc
     7261 ctcgacgtct cggagctggc gatggcggag ttgcccgaaa cgcccggcaa attcgcgatc
     7321 gtcccgggca acatagatcg cagcgagctc gtgagccgca ttcgcgccac gaacgtcgac
     7381 gaccgcatgc cgcccgagtc gacgcacaag acgctctcgg cgcagcaaat ggccatcctc
     7441 gagcaatgga tcgcgaacgg cgcgg
//
LOCUS       DATLEE010000038         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4510394, whole genome shotgun sequence.
ACCESSION   DATLEE010000038 DATLEE010000000
VERSION     DATLEE010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4510394"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VM692_00820"
     CDS             complement(<1..128)
                     /locus_tag="VM692_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HUQ50729.1"
                     /translation="MTLRIGDIAPDFEADTTEGRIRFHDWIGNSWAVLFSHPKDFTP"
     gene            195..1034
                     /locus_tag="VM692_00825"
     CDS             195..1034
                     /locus_tag="VM692_00825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ50730.1"
                     /translation="MLSLNRHPDARAFLLRTEQWLTTREIEHAGVLQSARQARGNDSH
                     YERPMYWTTIEDDGRLIGCAYRTPPYKVGVTALPEAALAPLIADLAATYSGAIGGFSG
                     PDPTVTELARAWVRQRGGSWSVNTGGRLLSFGSPGSAGESGAAGVLRLAAAGDSARAQ
                     SWGSAASIDSGIAALDGRLCLQLLGAKMLYFWEDDQPRCMLGLLRETPDSVAVGIVYT
                     PAAFRGEGHATAAIRALSRLLDERGVKNRYLWIDPKSDAANALAAKLGCGFVYDSLDI
                     DCT"
     gene            complement(987..>2488)
                     /locus_tag="VM692_00830"
     CDS             complement(987..>2488)
                     /locus_tag="VM692_00830"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HUQ50731.1"
                     /translation="PVIGHLRFLLEFVRPEIRQYFIESDNEQTPFSRQQRSIVYQRAK
                     GAPDKRPFGTQLDQHVAGYEWINHSLVPTEIATVDFRVKVGSAPSCTQPYDVSILNVS
                     AMSFGALSGNAILALNGGAKKGGFAHDTGEGSISPYHRVHGGDLIWEVASGYFGCRDE
                     HGRFDEAKFVANATDAQVKMIEIKLSQGAKPGHGGVLPGSKVTAEIAVTRGVRPGEDC
                     VSPARHSEFSTPVELLQFVERLRKLSGGKPTGFKLCIGHPWEWFAICKAMLETKLTPD
                     FIVVDGSEGGTGAAPLEFVDHVGTPLQEGLLLVHNTLVGLNLRHRIKLGCSGKIISAF
                     DIARALALGADWCNSARGFMFALGCLQAQTCHTGNCPTGVATQDPLRQRALAVPEKLE
                     RVYRFHHHTLHALRELAQAAGLKHPNQFRAAHLVRRVPDNDVRLLSNVLPQVPSGVLL
                     EAAAGRAKWPHRVFALYWPQADSRSFAPLKVDAASGAVDVERVVHESAA"
BASE COUNT          389 a          811 c          864 g          424 t
ORIGIN      
        1 ggggtgaaat ccttcggatg cgagaaaagg acggcccaac tgttgccgat ccagtcgtgg
       61 aagcgaatgc ggccttcggt cgtgtccgcc tcgaaatcgg gagcgatatc cccaatgcgc
      121 agtgtcatgg ttgttctccg cttcgctagg tgtgaaggcc gattctagcc cttgcgcggt
      181 atagtgttga gcccatgctg agcctgaacc gtcatcccga cgcccgcgca tttctgctgc
      241 gcaccgagca gtggttgact acgcgcgaaa tcgagcatgc cggtgtgctg caaagcgctc
      301 ggcaagcgcg tggcaacgac agccactacg agcgcccgat gtactggacg acgatcgagg
      361 acgacggccg gcttattggg tgcgcgtatc ggacgccgcc gtacaaggtc ggagtgacgg
      421 cgctgcccga agccgcgctt gcgcctctga ttgccgactt ggcggctacg tattccggcg
      481 ccatcggggg cttcagcggc ccggacccga cggtcaccga gctcgcgcgc gcttgggtgc
      541 gccagcgcgg cggctcgtgg tcggtcaata ccggcggcag gctgctgtcg tttgggtcgc
      601 ccggctcggc cggcgaatcg ggcgctgccg gcgtgctgcg gctggcggcg gccggcgact
      661 cggcgcgcgc gcagagttgg gggtcggccg cgtcgatcga cagcggcatc gcagcgctcg
      721 acgggcgcct ctgcctgcaa ttgctcggcg ccaagatgct gtatttctgg gaggacgatc
      781 agccgcggtg catgctcggc ctgttgcgcg agacgccaga ttcggtcgcc gtcggcatcg
      841 tctacacgcc ggccgcgttc cgcggcgagg gtcatgccac ggctgcgatt cgcgcgctga
      901 gccggctgct cgacgagcgc ggcgtcaaga atcgctatct gtggatcgat cccaaaagcg
      961 acgcggcgaa cgcgctggcg gccaagctag gctgcggatt cgtgtacgac tcgctcgaca
     1021 tcgactgcac ctgacgccgc atcgactttg agcggcgcga agctgcgcga gtcggcctgc
     1081 ggccagtaga gcgcgaagac gcggtgcggc catttcgcgc gccccgcggc cgcctcgagc
     1141 agcaccccgg acggcacttg cggcagcacg ttcgacaaca gccgcacgtc gttgtcgggc
     1201 acgcgccgca cgagatgggc cgcgcgaaac tggttcggat gcttaaggcc cgcggcctgc
     1261 gcgagctcgc gcagcgcatg cagcgtgtgg tgatggaacc gatagacgcg ctcgagcttc
     1321 tcgggcaccg cgagcgcgcg ctggcgcagc gggtcctgcg tcgcgacgcc cgtcgggcaa
     1381 ttgcccgtgt gacaggtctg cgcctgcaag caaccgagcg cgaacatgaa gccgcgcgcc
     1441 gagttgcacc agtccgcgcc gagcgcgagc gcccgagcga tgtcgaaggc gctgatgatc
     1501 ttgccgctgc agccgagctt gatgcggtgc cgcagattca agccgacgag cgtgttgtgc
     1561 acgagcagca agccttcttg cagcggcgtg ccgacgtgat cgacgaactc gagcggcgcg
     1621 gcgcccgtgc cgccttcgga gccgtcgacg acgatgaagt ccggcgtcag cttggtctcg
     1681 agcatcgctt tgcagatcgc gaaccactcc cacgggtggc cgatgcacag cttgaagccc
     1741 gtcggcttgc cgcccgacag cttgcgtagg cgctcgacga actgcagcag ctcgacgggc
     1801 gtcgagaact cgctgtggcg cgcggggctc acgcagtcct cgccggggcg cacgccgcgg
     1861 gtcacggcaa tctccgccgt gaccttcgag ccgggcagca cgccgccgtg gccgggcttc
     1921 gcgccttggc tcaacttgat ctcgatcatc ttgacttgcg cgtccgtcgc gttcgcgacg
     1981 aactttgcct cgtcgaagcg gccgtgctcg tcgcggcagc cgaagtaacc ggacgcgacc
     2041 tcccagatca agtcgccgcc gtgcacgcga tggtacgggc tgatcgagcc ttcgcccgtg
     2101 tcatgcgcga acccgccctt tttggcgccg ccgttcagcg cgaggatcgc gttgcccgac
     2161 aacgcgccga agctcatggc cgagacgttc aagatgctga cgtcgtacgg ctgcgtgcag
     2221 ctcggcgccg agccgacctt gacgcggaag tccaccgtcg caatctccgt cggcacgagc
     2281 gagtggttga tccactcgta gcccgcgacg tgctggtcga gctgcgtgcc gaacggccgt
     2341 ttgtcgggcg cgcctttggc acgctgatag acgatcgagc gctgttggcg cgagaacggt
     2401 gtttgctcgt tgtcgctctc gatgaagtac tggcgtatct cggggcgaac gaactcgagc
     2461 aggaaacgca ggtggccgat gacgggat
//
LOCUS       DATLEE010000039         3356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9708590, whole genome shotgun sequence.
ACCESSION   DATLEE010000039 DATLEE010000000
VERSION     DATLEE010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9708590"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1135
                     /gene="msrP"
                     /locus_tag="VM692_00835"
     CDS             179..1135
                     /gene="msrP"
                     /locus_tag="VM692_00835"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HUQ50732.1"
                     /translation="MRKRLDTGIKASEITPESTYLNRRTLLQAAVAAGLLPSIMSRGE
                     AATIPASGMFENVKKWPESTTDKANTFQDITTYNNYYEFGTGKADPAENAHTLKTSPW
                     SVQVTGEAGKTGTYTLEDILKPHDIEERIYRHRCVEAWSMVIPWNGFALADLLKRFEP
                     TSNAKYVQFFTLNDRTQMPGVRYPVLDWPYREGLTIAEAMHPLAFVAVGVYGKVLPNQ
                     NGAPLRTVLPWKYGFKGTKSIVRIHFTDRQPETSWNMAAANEYGFYSNVNPQVDHPRW
                     SQATERRLGGGLFSPRIPTQMFNGYTDQVASLYTGLDLRKNY"
     gene            1163..1858
                     /locus_tag="VM692_00840"
     CDS             1163..1858
                     /locus_tag="VM692_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HUQ50733.1"
                     /translation="MVVTKIALWAACLAPLALLILQALNDLRPGGLFQAFGSLGANPV
                     EQILNTCGKTGLNMLVLTLCITPIRRSTGINRLILFRRLFGLFAFFYLSLHFLTYTLL
                     DLGLLYAPGEAGATLLVDIAERPYITLGFTALLLLIPLAVTSTRGMQRRLGRNWVKLH
                     RLVYVVVVLGVVHYYWQVKSDLDFGQPLLYAFVFALLLGVRVHHWLVHTKTAVAPSYE
                     GVKPPQASGKPSH"
     gene            complement(1778..2374)
                     /locus_tag="VM692_00845"
     CDS             complement(1778..2374)
                     /locus_tag="VM692_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HUQ50734.1"
                     /translation="MRKAVFIAVALSGWPTVAAAETAYVTDSLRLGLHAAPDTSDRPF
                     ENLQSGTAVEVLERNPNYARVRLGDGREGWVKATFLVALKPAAARLGELEAEMERLNE
                     AAAAAKTAQATAEQELARYTAELQARTGSAETVQETVERLQQENRAHEQRLEAYRYAL
                     PMTWVIPALVVVLVAGFAAGLWWLDALIRRRHGGFRVY"
     gene            complement(2457..>3356)
                     /locus_tag="VM692_00850"
     CDS             complement(2457..>3356)
                     /locus_tag="VM692_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ50735.1"
                     /translation="LAAAELACKRAKSEGGARVAAVDATATLTPVATRQALAATELSE
                     ALQSNQFQLDAQPIVDLRGAGTVGFELLVRLRNPAGQLLAPNKFLAACAQYGLMPALD
                     RWVLSAAVEALRPHAAILESSSPFFAVNVSAQSLEHRGYAAFAVEALAAAGLPASLFC
                     FEIKEAAAVAQLSAADALIRTLLQAGAKVALDDFGSGLSSLAHLKQLPVSYLKIDGRF
                     VRSMIGDRIAESIVSGIARAAYTLGVATIAEHVETAAVVNRLSELDVTLGQGFELGRP
                     QPIAHAVQQAVRHAAAAMPGLRA"
BASE COUNT          584 a         1097 c         1072 g          603 t
ORIGIN      
        1 tatcagcttc atgagtcccc ctaagacttt tgcaaagacc gcctacgaga agtgtgcccc
       61 ggttggggcg ccgttgttgt gtattgttat tacaggggta ttctagccaa gtcgcgcagt
      121 tcgtggcgac gccggcgcga ccggcatttt ccccgcagat gagatgtagg tgaaagtcat
      181 gcgtaaacga ttggacaccg gaatcaaagc gtcggaaatc acgccggaaa gcacgtacct
      241 taatcgtcgc acgctgttgc aggcggccgt cgctgcgggc ttgttgccgt cgatcatgtc
      301 gcgtggcgaa gccgcgacga ttcctgcgag cggcatgttc gagaacgtca agaaatggcc
      361 cgagtcgacg accgacaagg ccaacacgtt ccaggacatc acgacctaca acaactacta
      421 cgagttcggc acaggcaaag ccgatcccgc cgagaatgcc cataccctga agacctcgcc
      481 gtggtccgtg caggtcacgg gcgaagcggg caaaacgggc acctacacgc tcgaagatat
      541 cttgaagccg cacgacatcg aggagcgcat ttaccgccat cgctgcgtcg aagcgtggtc
      601 gatggtcatc ccgtggaacg gcttcgcgct ggccgatctc ttgaagcggt tcgagccgac
      661 gtcgaatgcg aaatacgtgc agttcttcac tttgaacgac cgcacgcaga tgccgggcgt
      721 tcgttatccc gtgctcgatt ggccctatcg cgaaggcttg acgatcgcag aagccatgca
      781 cccgctcgcg ttcgtcgccg tcggcgttta cggcaaagtg ctgccgaacc agaacggcgc
      841 gccgctgcgt accgtcctgc cgtggaagta cggcttcaag ggcacgaagt cgatcgtccg
      901 tatccacttc acggaccggc agccggagac gagttggaac atggcggcgg cgaacgagta
      961 cggtttctac tcgaacgtca atccgcaagt cgatcacccg cggtggagcc aagccacgga
     1021 acggcgtctc ggcggcgggc tgttctcgcc gcgcatcccg acgcaaatgt tcaacggcta
     1081 caccgatcag gtcgccagcc tctacacggg cctcgacctg cgcaagaatt attgacgcgg
     1141 tctgccgatg acgccgcgct gggtggtcgt tactaagatc gccctatggg cagcgtgctt
     1201 ggcgccgctg gccctgctga tcctgcaagc gctgaacgac ctgcgcccgg gcggcctttt
     1261 tcaggcgttc ggctccctcg gcgccaaccc cgtcgagcag attttgaaca cgtgcggcaa
     1321 gaccgggttg aacatgttgg ttctgacgct gtgcatcacg ccgatccggc gctcgaccgg
     1381 tatcaacagg ctcatcttgt ttcgccgcct gttcggcttg ttcgcgttct tctatctgtc
     1441 gctgcacttc ctgacgtata ccctgctcga cctcggcctc ttgtacgcgc ccggcgaagc
     1501 cggcgcgacg ctgctcgtcg acatcgcgga gcggccgtac atcacgttgg gattcacggc
     1561 attgctgttg ctgatcccgc tcgcggtgac gtcgacgcgc ggcatgcagc ggcgcttagg
     1621 acgaaactgg gtgaagctgc accgtctcgt ctacgtcgtc gtcgtgctcg gcgtcgtgca
     1681 ctactactgg caagtgaaat cggatctcga tttcggccag ccgctgctct acgcgttcgt
     1741 gttcgcgctg ttactcggcg tgcgagtgca ccattggcta gtacacacga aaaccgccgt
     1801 ggcgccgtcg tatgagggcg tcaagccacc acaagccagc ggcaaaccca gccactagca
     1861 cgacgacgag cgcgggaatc acccacgtca tcggcagcgc gtagcggtag gcctcgagcc
     1921 gctgctcgtg cgcgcggttt tcctgttgca gcctctcgac tgtttcctgc accgtctcgg
     1981 ccgagccggt tcttgcttgc agctcggccg tataccgcgc gagctcttgc tcggcggttg
     2041 cctgcgccgt cttggccgcg gccgctgcct cgttgaggcg ctccatctcg gcctcgagct
     2101 cccccaggcg cgcggccgcc ggcttcagcg caacgagaaa cgtcgctttg acccagccct
     2161 cgcggccgtc gcccaagcga actcgcgcgt agttcgggtt gcgctcgagt acctcgacgg
     2221 ccgtgccgct ctgcagattc tcgaacgggc ggtcgctcgt gtcgggcgcc gcgtgtaagc
     2281 cgagccgcaa gctgtcggtg acataggccg tctcggcggc cgcaacggtg ggccaccccg
     2341 acagcgccac ggctatgaac acagctttcc gcatgccgcc cccggctttg acagaatctg
     2401 cgcggattat aggtgccgcc gcggcggcgc ggatgtgatc caagtggcga gaagcgctac
     2461 gcgcgcaagc ccggcatggc cgcggcggcg tgccgaacgg cctgctgcac ggcgtgcgcg
     2521 atgggctgag gccggccgag ctcgaagccc tgccccagcg tgacgtcgag ctcgctcaat
     2581 cgattgacga ccgcggcggt ctcgacgtgc tcggcgatcg tcgccacgcc gagcgtatac
     2641 gcggcgcgcg caatgcccga gacgatcgac tcggcgatgc ggtcgccgat catgctgcgg
     2701 acgaaccggc cgtcgatctt cagatagctg accggcaatt gcttcagatg ggccagcgag
     2761 ctcaagcccg agccgaagtc gtcgagcgcg actttggcgc cggcttgtag cagggtgcga
     2821 atcagtgcgt cagccgcgct cagttgcgcg acggcggcgg cctccttgat ctcgaagcag
     2881 aacaggcttg ccggtaggcc ggcggcggcc agcgcctcga cggcgaacgc ggcgtagccg
     2941 cgatgctcga gcgactgcgc agaaacgttg acggcaaaaa aaggcgacga gctctcgaga
     3001 atcgcggcgt gcggccgcag agcttcgaca gccgcgctca ggacccagcg gtcgagcgcg
     3061 ggcatcaagc cgtactgcgc gcaggccgcg aggaacttgt tcggtgcgag cagctggccc
     3121 gcgggattgc gcaagcgcac gagcagctcg aacccgacgg ttccggcacc gcgcaggtcg
     3181 acgatcggct gcgcgtcgag ctggaattga ttcgactgca gcgcctcgct gagctccgtc
     3241 gcggccaacg cctgccgcgt cgcaacgggc gtcaacgtcg ctgtggcatc gacggcggcc
     3301 acgcgcgcgc cgccctcgga cttcgcccgt ttgcaggcga gctcagcggc cgcgag
//
LOCUS       DATLEE010000040         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5261619, whole genome shotgun sequence.
ACCESSION   DATLEE010000040 DATLEE010000000
VERSION     DATLEE010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5261619"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VM692_00855"
     CDS             complement(<1..1016)
                     /locus_tag="VM692_00855"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HUQ50736.1"
                     /translation="MARPLADLDFSEPIRLAAALQTGKKVPPDVTGLLAEDHRVVLGW
                     FRWYEATTELPVRRRLVERLCLALRAHMAGEEEFFYPALREADSGAPSVEHAIAEHAQ
                     AKRIIEQLEQSPDEAAATDGLVAKLKNEIVAHVAEEETQLFPLARQVPIDLYEVGSAV
                     AACRAATLLKLKARGPSPATVTEEEFPVMTISQADARDYFLVGLKNAHATTRQGRVLI
                     AGQLERVESYPQLKEKLATHLREKDAQLERLERLIGGYGDKPSVVKDTAMSVAAGVAA
                     IASAAAADEIIKNSFATLAQAKYEAAAFETLIVFAQAAGEDAALRPLQQCLSESRALG
                     SFVE"
     gene            complement(1026..>2205)
                     /locus_tag="VM692_00860"
     CDS             complement(1026..>2205)
                     /locus_tag="VM692_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50737.1"
                     /translation="KDDVHPYSRARGILLNGIEVEAVMFSHQMARNTLDATIKRQLAM
                     LRRVEAQQQKAINWLIPGDETTIEVTLGYEQVAVDLTAWLAQHEADPYLKQVYDFALL
                     EDFDHLYRYANLYELIEGGKADEICGDLTEITPGRPTIFEHRDPSDEIRRPMTAQAAD
                     PQSVLNALTIVAAEQQTMNFYMTIGNRYQEPIARATYAEIALIEEQHVSHYESILDPS
                     ASWFENLVLHQWHECWMYWSAAQDETDARVRKLYDRHLGMEIEHLRVACELMKRVERR
                     DPEEILPLTGFSEPLRFQPNKAYVRAVLASQIDLTAKDSDFYPVSTLLEDDRYFAYQQ
                     RVNGDFSPTEEVIRANKEKNGRDYRLETEGPNPVEGLRSDDERNGAATEYARRQQAAA
                     "
BASE COUNT          366 a          716 c          683 g          440 t
ORIGIN      
        1 tcctcgacga agctgccgag cgcgcgcgac tcgctcaagc attgttgcag cggccgcagt
       61 gccgcgtcct cgccggcggc ctgtgcgaag acgatcagcg tctcgaacgc cgcggcctcg
      121 tacttcgcct gcgccagcgt cgcaaagctg ttcttgatga tttcgtctgc agctgccgcg
      181 ctcgcaatag ctgcgacgcc cgccgcaacc gacatcgccg tgtccttgac gaccgacggc
      241 ttgtcgccgt agccgccgat caagcgctcg agacgctcga gctgcgcatc tttctcgcgc
      301 agatgcgtgg cgagcttctc tttcagctgc ggatagctct cgacgcgttc gagctggccg
      361 gcgatcagca cccggccttg ccgcgtcgtc gcgtgagcgt tcttgagccc aactaaaaag
      421 taatcacgtg catcagcctg cgagatggtc atgacgggga actcctcttc tgtaacggtg
      481 gccggcgatg ggccgcgcgc tttcagcttc agcagcgtgg cggccctgca tgccgcgacg
      541 gcgctgccga cctcgtagag gtcgatcggc acctgccgcg ccagcgggaa cagctgcgtc
      601 tcctcttccg ccacgtgggc cacgatctcg ttcttcagct tcgcgaccag accgtccgtc
      661 gcagccgctt catcgggcga ttgctcgagc tgctcgatga tccgtttcgc ctgagcatgc
      721 tcggcgatcg catgctcgac ggacggcgcg ccggagtcgg cctcacgaag agccggatag
      781 aaaaactcct cttcgccggc catgtgcgca cgcagtgcga ggcaaaggcg ctcgacgagg
      841 cggcggcgga cggggagctc cgtagtcgcc tcataccagc ggaaccagcc gagcacgacg
      901 cgatgatctt cggccaagag ccccgtgacg tcggggggta ctttcttgcc ggtctgtaac
      961 gcggcggcca aacgaatcgg ctccgagaaa tccaagtcgg ccaacggacg tgccatgact
     1021 tcctcctagg cggcggcttg ttgccgtcgc gcgtactcgg tcgcggcgcc gttgcgctcg
     1081 tcgtcgctgc gcaagccctc gacgggattc gggccttcgg tctcgaggcg atagtcgcgt
     1141 ccgttcttct ccttgttcgc gcgaataacc tcttccgtcg ggctgaagtc gccgttgacg
     1201 cgctgttgat aggcgaagta gcgatcgtcc tcgagcaacg tcgagacagg gtaaaaatcc
     1261 gagtcctttg ccgtgaggtc gatctggctc gcgagcacgg cgcgcacgta ggccttgttc
     1321 ggctggaacc gcagcggctc gctgaagccg gtcagcggca agatctcctc gggatcgcgc
     1381 cgctcgacgc gcttcatgag ctcgcaagcg acacgcaaat gctcgatctc catgccgaga
     1441 tggcgatcgt acagcttgcg aacgcgcgca tcggtctcgt cttgcgcggc cgaccagtac
     1501 atccagcact cgtgccattg atgcagcacg aggttctcga accagctcgc ggacggatcg
     1561 aggatcgact cgtaatggct gacgtgctgc tcctcgatga gagcgatttc cgcgtacgtc
     1621 gcacgcgcga tcggctcctg atagcgatta ccgatcgtca tgtagaaatt catcgtctgt
     1681 tgctcggccg ctacgatcgt cagagcattc aagacggatt gcggatcggc cgcctgcgcc
     1741 gtcattggcc tgcgaatctc gtcgctcggg tcacggtgct cgaagatcgt cggccggccc
     1801 ggagtgatct ccgtcaagtc gccgcagatt tcgtcggcct tgccgccttc gatgagctcg
     1861 tagagattcg cgtagcgata gagatgatcg aaatcctcca gcagcgcgaa gtcgtagacc
     1921 tgcttcagat aggggtccgc ctcgtgctgc gcgagccagg ccgtcagatc cacggccacc
     1981 tgctcgtaac cgagcgtgac ctcgatcgtc gtttcgtcgc cggggatcaa ccagttgatg
     2041 gctttctgtt gctgcgcctc gacgcggcgc agcatcgcga gctgacgctt gatcgtcgca
     2101 tccagcgtgt tgcgggccat ctggtgcgag aacatgaccg cctcgacctc gatgccattc
     2161 aataatatgc cgcgcgcgcg gctgtacgga tgcacgtcgt ccttg
//
LOCUS       DATLEE010000041         5319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3771624, whole genome shotgun sequence.
ACCESSION   DATLEE010000041 DATLEE010000000
VERSION     DATLEE010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3771624"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VM692_00865"
     CDS             <1..586
                     /locus_tag="VM692_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50738.1"
                     /translation="GLMRQPGRLHITWADDDTLQVDFDAGTQTRLFEFGTAQKPSAEP
                     TWQGFSRAEWQRPPGGNQGPVRAQIGNSTGPIAPGGGGRGQRGGPPPSLGSLTEGGSL
                     KAVTTGFRSGYLRKNGVPYSEQATITEYITRLPEAPNGEDWLHVLTIVEDPRYLNDAF
                     YTSTHFKREPNGSKWNPTECRTAPPPPPAPAPAR"
     gene            697..1635
                     /locus_tag="VM692_00870"
     CDS             697..1635
                     /locus_tag="VM692_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50739.1"
                     /translation="MRNLLVAAVVLGVGGYFGAKFYVQYKTAKDLDAVLTQARPMVDI
                     QYENVVATMGGELRVEGVTLHVPQFDDEITIESVGLQTPGFLFLLGFDQRKVEMPERL
                     GVSLSGMRMRADSDFMRALDNDPKTVARRAALTPADQCATSYGFSSAMLQQLGYHDVV
                     MDFSMEFRRQGSRVVMGFGADVENMYDIDIELTLDGIADPTELARGARPLLVGARIDY
                     VDQSLNNRIMKYCMEQEVTAEDVIAAQLREVHQLARDTGMELDAMIIQPYTDFLLGKQ
                     RFTLIAQPINPVDLTQLSLYKPSDVPNLLNLTAEAG"
     gene            1727..2590
                     /locus_tag="VM692_00875"
     CDS             1727..2590
                     /locus_tag="VM692_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HUQ50740.1"
                     /translation="MFAAKRHPLLVPFDASFDVREAPTKAKTQRDWKELLATEVKALG
                     ESQDRLFADGRYAVLLVFQALDAAGKDGTIQHVFSGVNPSGLHVASFKQPTSRELAHD
                     FLWRTTAELPERGSIAIFNRSYYEEALVVRVHPEYLAAQKLPEPPPKTFWDERLRAIA
                     DHERFLAEQGTVILKFWLNVSKAEQRARLLERIDEPKKNWKFNPRDLDERALWDEYLD
                     AYDACLSATSRPWAPWYAVPADDKHYARWQVANLVNEMLEQLDVDFRPVDKETKAELK
                     KAKARLLAEKD"
     gene            complement(2594..4966)
                     /locus_tag="VM692_00880"
     CDS             complement(2594..4966)
                     /locus_tag="VM692_00880"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HUQ50741.1"
                     /translation="MLCLFAASGCAALIYQVVWFEQLTLAIGSSAVSLGVLLATFLGG
                     LGVGSLLASRMTSSRSAPLRRYALLELAVGVLGLVALWSVPLLGGAYAALAGTGALSL
                     GLRLAVAVLTLLPATLLMGATLPIVAAVLRADASGAAWLGYCYAANTVGGVAGSVVAG
                     FYLLRVHDAYVATFIAAALNLGCAAVAMALARGDALPGAARSAAVPAVQARTAASTRA
                     VYVATLLSGMTALAAEVLWTRHLTLLLGGTVYTFALIVAVFLAGIGLGSVAGAAAGRR
                     FEPRSALAACQAALGIAMASAAFALAESLPYWPLDVSLPSTASIALQLDTLRTAIVAL
                     PSALLWGASLPLALAAVVAAGEQPQRAIGRLYAANTLGAILGALGTTFVLVVAIGSQR
                     TQQLMIVVSGVAALLVLRSTVGTRRFAAAAAVVAALTLAALSVQPIPGALVAFGRFLP
                     TRGFDANVIYVGEGLTASVAVSEEPNGTLTYHNAGKTQASTYAEDMRLQRMLGHLTTL
                     LVAQPRDVLVIGLGAGITAGAVSIDPAVERIVVAELEPLVPEVASEYFGAHNFAVVSS
                     PKVEIRIDDGRHFLATTADRFDAITSDPLDPWVKGAAALYTREFWQLCKARLRDGGAV
                     TVFVQLYETTEDAVRSELATFLDVFPNGAVFANTVEGTGYDAVLVGRKSDEPIDLTRL
                     QQRFESSSHARVAESLRGVGFDSALDLMSTFAGDAHSLTPWLEGAQRNTDRTLRLQYL
                     AGQGLNVLAAGEIYEALVGATPHLPVELFTGTPAQLEELEQRLAGRRGQY"
BASE COUNT          931 a         1809 c         1805 g          774 t
ORIGIN      
        1 cggcctgatg cgccagccgg gccgcttgca catcacgtgg gctgacgacg acaccttgca
       61 ggtcgacttc gacgcgggca cccaaacgcg cctgttcgag ttcggcaccg cgcagaagcc
      121 gagcgccgag ccgacgtggc aaggcttctc gcgcgccgaa tggcaacggc cgccgggcgg
      181 caatcaaggc cccgtgcgcg cgcagatcgg caacagcacg ggcccgatcg cgccgggggg
      241 cggcgggcgc ggtcaacgcg gcggtccgcc gccgtcgttg ggctcgctga ccgaaggcgg
      301 ttcccttaag gccgtcacga cgggtttccg cagcggctac ctgcgcaaga acggcgtgcc
      361 gtacagcgag caggcgacca tcaccgaata catcacgcgt ctgcccgagg cgccgaatgg
      421 cgaggactgg ttgcacgtgc tgacgatcgt cgaggatccg cgctacttga acgacgcgtt
      481 ctacacgagc acgcacttca aacgcgagcc gaacggcagc aagtggaatc cgacggagtg
      541 ccgcacggcg cctccgcccc cgcctgcacc tgcacctgca cgttaatccc caggggccgc
      601 ctccgccgcg gcgtttctgt gactaccgtc gccgcgggcc cggcggctcg tacctagact
      661 ggctcattcc tggccttttt aagaggcgga gcgagcatgc gcaatctact cgtagcggcg
      721 gtcgtcctcg gcgtcggcgg ctacttcggc gcgaaattct acgttcaata caagacggcg
      781 aaggatctcg acgccgttct cacgcaagct cgacccatgg tcgacatcca gtacgagaac
      841 gtcgtcgcaa cgatgggcgg cgagctgcgc gtcgaaggcg ttaccttgca cgtgccgcag
      901 ttcgacgacg agatcacgat cgagtccgtc ggcctgcaaa cgccgggctt cctctttctg
      961 ctcggcttcg accagcgcaa ggtcgagatg ccggagcgtc tcggcgtttc gctgtccggc
     1021 atgcgcatgc gcgcggactc ggatttcatg cgagcgctcg acaacgatcc caagacggtc
     1081 gctcggcggg cggcgctgac gccggccgac caatgcgcga cgtcgtacgg cttctcgtcc
     1141 gcgatgctgc agcagctcgg ctaccacgac gtcgtcatgg acttcagcat ggagtttcgg
     1201 cgtcagggct cgcgagtcgt catgggcttc ggggcggacg tcgagaacat gtacgacatc
     1261 gacatcgagc tgacgctcga cggcatcgcg gatccgacgg agctcgcgcg cggcgcgcgg
     1321 ccgttgctcg tcggcgcgcg catcgattac gtcgatcagt cgctgaacaa tcgcatcatg
     1381 aagtactgca tggagcagga agtgacggct gaggacgtca tcgccgcgca attgcgcgaa
     1441 gtgcatcagc tggcacgcga caccggcatg gagctcgacg cgatgatcat ccagccctac
     1501 acggactttt tgctcggcaa gcaacgattc acgttgatcg cgcagccgat caaccccgtc
     1561 gacctcacgc agctctcgct gtacaagccg agcgacgtgc cgaacctgct gaatctcacg
     1621 gccgaagccg gctagcgcta caggcgcccg ccgcgtcggg tacgatcagg aatagccccg
     1681 gcgctgcgcg cgccgttcct cgttcccgtc gttgccgagg tatctcatgt tcgcggccaa
     1741 acgccacccg ctgctcgttc cgttcgacgc ctcgttcgac gtccgtgagg cgccgacaaa
     1801 ggctaagacc caacgcgatt ggaaggagct gctggcaacc gaggtcaagg cgctcggtga
     1861 gtctcaagac cggttgtttg ccgacgggcg ctacgccgtg ctactcgtgt ttcaggcgct
     1921 cgatgccgca ggcaaggatg gcacgatcca gcacgtgttc tcgggcgtga acccgtcggg
     1981 cttgcacgtc gcgtcgttca agcagccgac gagccgcgag ttggcccacg acttcctttg
     2041 gcgcacgacg gccgagctgc ccgagcgcgg cagcatcgca atcttcaatc gcagctacta
     2101 cgaggaagcg ctcgtcgtgc gagtgcatcc cgagtacctc gcggcgcaaa agttgccgga
     2161 gccgccgccg aagacgttct gggacgagcg gctgcgagcg atcgcggacc acgagcgctt
     2221 tctcgccgag caaggcacgg tgatcttgaa gttttggctc aacgtctcga aggcggagca
     2281 gcgggcccgc ttgctcgagc gaatcgacga gcccaagaag aactggaagt tcaatcctcg
     2341 cgatctcgac gagcgagcac tgtgggacga gtacctcgac gcatacgacg cctgtttgag
     2401 tgcgacgtcg cggccgtggg cgccatggta tgccgtgccg gccgacgaca agcactacgc
     2461 gcgctggcaa gtcgcgaatc tcgtcaacga gatgctcgag cagctcgacg tcgacttccg
     2521 gcccgtcgac aaggagacga aggctgagct caagaaggcg aaagcgcggc tgcttgccga
     2581 gaaagactga gcgtcagtac tgcccgcggc gccccgccag acgttgctct agctcctcga
     2641 gctgcgccgg ggttcccgtg aacagctcca ccggcaagtg cggcgtagcg ccgacgagcg
     2701 cttcgtagat ctcgccggcc gcgagcacgt tgaggccctg gcccgcgaga tactgcaggc
     2761 gcagcgtgcg atccgtgttg cgctgcgcgc cctccagcca tggcgttagc gagtgcgcat
     2821 cgcccgcgaa cgtgctcatg agatcgagcg ccgagtcgaa gcccacgccg cgcagcgatt
     2881 ccgccacgcg cgcgtggctc gagctctcga agcgctgctg cagtcgcgtc agatcgatcg
     2941 gctcgtcgct cttgcggccg acgagcacgg cgtcgtaacc cgtgccctcg acggtgttcg
     3001 cgaacacggc gccattcggg aacacgtcga ggaacgtcgc gagctcgctg cgcacggcgt
     3061 cctcggtcgt ctcatagagc tgcacgaaca ccgtgaccgc gccgccgtcg cggagccgcg
     3121 ctttgcataa ctgccagaac tcgcgcgtgt acagcgcggc ggcgcctttg acccaagggt
     3181 cgagcgggtc cgacgtgatc gcgtcgaagc gatcggccgt cgtcgccagg aaatggcgcc
     3241 cgtcgtcgat gcgaatctcg actttggggc tcgatacgac cgcgaagttg tgcgcgccga
     3301 agtactcgga ggcgacttcg ggaaccagcg gctccagctc cgcgacgaca attcgctcga
     3361 cggcaggatc gatgctgacg gcgcccgccg tgatgccggc tcccaagccg atcacgagca
     3421 cgtcgcgcgg ttgtgcgacg agcaaggtcg tgagatggcc cagcatgcgc tgcaggcgca
     3481 tgtcctcggc atacgtcgac gcctgcgtct tgccggcgtt gtgataggtc agcgttccgt
     3541 tcggctcctc ggaaacggcg accgacgcag tcaaaccttc gccgacgtag atgacgttgg
     3601 cgtcgaagcc gcgcgttggc aggaaccggc cgaatgcgac gagcgcgccg ggaatcggct
     3661 gcaccgagag cgcggccagc gtgagcgccg ccacgacggc ggccgctgcc gcgaagcgcc
     3721 gcgttccgac tgtggagcgc agcaccagaa gcgctgcgac gccgctgacg acgatcatga
     3781 gctgctgcgt acgctggctg ccgatcgcga cgacgagcac gaacgtcgtg ccgagcgccc
     3841 ccagaatcgc gcccagcgtg ttcgccgcat acaagcggcc gatcgcgcgc tgcggctgct
     3901 cgcccgccgc gactacagcc gcgagcgcca gcggcaagct cgcgccccaa agcagcgccg
     3961 agggcagggc gacgattgcc gtgcgcaagg tgtcgagctg cagcgcgatg gaggcggtcg
     4021 agggcagcga gacgtcgagc ggccagtaag gcagcgactc tgcgagcgcg aaggccgcgc
     4081 tggccatcgc gatgccgagc gcggcttggc acgcggcgag cgccgagcgc ggctcgaagc
     4141 ggcgcccggc cgcggcgccc gctacgctgc cgagcccgat gcccgccagg aagaccgcga
     4201 cgatcagcgc gaacgtatag acggtgccgc cgagcagcag cgtcagatgc cgtgtccaga
     4261 gcacctcggc cgcgagcgcc gtcatgcccg acaacagcgt cgcaacatag acggcgcgcg
     4321 tgcttgcggc ggtgcgcgct tggaccgccg gcaccgcggc gctccgcgcc gcgccgggca
     4381 gtgcgtcgcc gcgcgcaagc gccatggcga cggccgcgca gccgaggttc agcgcggcag
     4441 cgatgaacgt cgcgacgtag gcgtcgtgca cgcgcagcag atagaacccc gcgacgacgc
     4501 tgccagcgac gccgccgacg gtattggcgg cgtagcaata gccgagccag gcggcgccgc
     4561 tcgcatcggc gcgcaagacg gcggcgacga tcggcaacgt tgcgcccatc aatagcgtcg
     4621 ccggcaacag cgtcagcacc gcgacggcga gcctaagccc tagcgagagc gcgcccgtgc
     4681 ccgcgagcgc cgcgtaggcg ccgccgagca gcggcacgct ccagagcgcg acgagcccca
     4741 gcacgccgac tgcgagctcg agcaacgcat agcggcgcag gggcgccgag cgcgagctcg
     4801 tcatgcggct ggcaaggagg ctgcccactc ccaggccccc caagaacgtc gcgagcagca
     4861 cgccgagcga caccgccgag gagccgatcg ccagcgtcaa ttgctcgaac cagacgacct
     4921 gataaatcaa cgccgcacag ccgctggccg cgaacaggca cagcacggcg acgagcgtgc
     4981 cgcctgccgg ggcggcgtga gcgggggatg acgcgcagtc tgttggcacg atggtcgagg
     5041 gcggcgaatc gagtgcgaat tgcgccagca agcccgctgc attgcaacgg gcgcggcgcg
     5101 ttttgccgct tcgccgactc ctggtgcaga atgctgcgca agggggatcc atatgcagcg
     5161 cacgactccg ttgccgaggc tggttcgcgc gggcgccttc gtgtccgtcg cgttgttgct
     5221 gggctgcgag cagggtgcac cgcccggcac gacggcggcg cagacggcgc agccgacggc
     5281 ggcatcttcg tccccagtcg ccggccggcc cgccggccg
//
LOCUS       DATLEE010000042         3146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4515664, whole genome shotgun sequence.
ACCESSION   DATLEE010000042 DATLEE010000000
VERSION     DATLEE010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4515664"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..750
                     /locus_tag="VM692_00885"
     CDS             163..750
                     /locus_tag="VM692_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50742.1"
                     /translation="MRSGLLALALIVVTAVASAQPPPRVRGVVQSLEGNTLVVATQNE
                     GVVRLTLTDMTGINGLERRTLADITDNTFIGATAVKDRNGRWQATEVHIFPESMRGAG
                     EGHYAWDLPDSTMTNGAATGIVAKGRNGTLNVRHAGGNVPIEVTRKTQIVGLTAGSRA
                     LLVPGASVMALAQPANGGATAVAVIAETNGVKPPM"
     gene            complement(763..1245)
                     /locus_tag="VM692_00890"
     CDS             complement(763..1245)
                     /locus_tag="VM692_00890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUQ50743.1"
                     /translation="MAQQQWTTPPAMTIDVKKNYNVSIETNRGTIELELYPQHAPQTV
                     NNFVFLAGQGFYDGVKFHRVIDDFMIQTGDPTGTGRGGPGYKFGDETKNNPLKHERCV
                     ISMANAGPNTNGSQFFITHSPQPHLNGKHTVFGKVTKGQDLVDSIEQGDEIVSVKVAS
                     "
     gene            complement(1321..1641)
                     /gene="yjgA"
                     /locus_tag="VM692_00895"
     CDS             complement(1321..1641)
                     /gene="yjgA"
                     /locus_tag="VM692_00895"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HUQ50744.1"
                     /translation="MARKSRDFEPDDAVEPNDDEPTEGRGRSARKRASHEITKLGQEL
                     VELRPERRTRLALPDWLEDAIVEARRLTSHGAQRRQAQFIGKLMRKLDEDSIAAIRKE
                     LERD"
     gene            complement(1657..2952)
                     /locus_tag="VM692_00900"
     CDS             complement(1657..2952)
                     /locus_tag="VM692_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUQ50745.1"
                     /translation="MSGSATHAAIEAVFRIEWPKLIAGLTRVVRDVGRAEELAQEALV
                     AALEQWPEEGIPVKPGAWLMQAAKHRAIDAVRHAKLVRRKHEELGGELEAMGGEASDE
                     REAIDEALDDQIGDDMLSLMFIACHPVLAMEARVALTLRMVGGLTTTEIARAFLVPEP
                     TIAQRIVRAKRTLAEARVPFEVPRGEELSERLASVLEVVYLVFNEGYSATAGDDWLRP
                     ALCDDALKLARMLAELAPREPEVHGLVALLEIQASRSAARTAANGEPVLLLEQDRGRW
                     DRLLIRRGLAALERAEALGGALGPYALQAAIAACNARAATAADTDWPRIVALYDALAA
                     LTPSPVVELNRAVAVAMAFGPAEGLSIVDELVAGGALKSYHLLPAVRGDFLAKLGRHA
                     EARLEFERAASLTSNERERALMTARAAACAALVAPPPLA"
     gene            complement(2949..>3146)
                     /locus_tag="VM692_00905"
     CDS             complement(2949..>3146)
                     /locus_tag="VM692_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUQ50746.1"
                     /translation="LRNFGGKQTVVDGPFAESKELVGGFAIVDLESRAEAIEWAQRYA
                     AVLGEVELDIRPVAGWSTFGQ"
BASE COUNT          486 a         1093 c         1047 g          520 t
ORIGIN      
        1 gatttcgcaa tactggcacg ggcgcttcca tcgcgagccc ggcaaccagt ggattcgcaa
       61 cgtgttcgcc aagctcttcg caggcgccgc cgacgacgga gtgcagccgc gctcggcctg
      121 acggatacta gtgcgccgct cgatccgttc gcgaggacag ctatgagatc cggtctgctc
      181 gctttggcgc tcatcgttgt cacggcggtg gcctcggctc agccgccgcc gcgcgtccgc
      241 ggcgtcgtgc aatccctcga aggcaacacg ctcgtcgtcg cgacgcagaa cgaaggcgtc
      301 gtgcgcctga cgttgacgga catgacgggc atcaacggcc tcgagaggcg cacgctggcc
      361 gacatcaccg acaacacgtt catcggcgcg acggccgtca aggatcgcaa cggccgctgg
      421 caagccaccg aggtgcatat ctttccagag tcgatgcgcg gcgccggcga aggccactac
      481 gcgtgggacc tgcccgacag cacgatgacg aacggcgccg caaccggcat cgtcgcgaag
      541 ggacgcaacg gcacgctgaa cgtgcggcac gccggcggca acgtgccgat cgaggtcacg
      601 cgcaagacgc agatcgtcgg cttgacggcc ggcagccgcg ccctgctcgt gccgggcgcc
      661 agcgtgatgg cgctcgcgca gcctgccaac ggcggcgcga cggccgtggc cgtcatcgcg
      721 gagacgaacg gcgtcaagcc gccgatgtag gaatgcgcga ggtcagctcg cgactttgac
      781 gctgacgatc tcgtcgccct gctcgatgga gtcgacgagg tcttggcctt ttgtgacctt
      841 gccgaacacc gtgtgcttgc cgttcaagtg cggctgcggc gagtgcgtga tgaagaactg
      901 gctgccgttc gtgttggggc ccgcgttcgc catcgagatc acgcagcgct cgtgcttcaa
      961 cggattgttc ttggtctcgt cgccgaactt gtagccggga ccgccgcgac ccgtgcccgt
     1021 cgggtcgccg gtctggatca tgaagtcgtc gatgacgcgg tggaatttca cgccgtcgta
     1081 gaaaccttgg ccggcgagaa acacgaagtt gttgaccgtt tgcggcgcgt gctgcggata
     1141 gagctcgagc tcgatggtgc cgcggttggt ctcgatcgac acgttgtagt tcttcttgac
     1201 gtcgatcgtc atcgccggcg gggtcgtcca ttgttgctgc gccatagagc tctcctttgt
     1261 tcgggccggc cacgataccg gacttgccgg tttcgagcca tcgaagcgcg ggccactgtt
     1321 tcagtcgcgc tcgagctcct tgcgaatggc ggcgatcgag tcctcgtcga gcttgcgcat
     1381 cagcttgccg atgaactgag cttggcgccg ctgcgcgccg tggctcgtga gccgccgcgc
     1441 ttcgacgatt gcgtcctcga gccagtccgg cagtgccaag cgggttcggc gttccggtcg
     1501 cagctcgact agctcctgcc ccagcttcgt gatctcgtgg ctggcgcgct tgcgcgcgct
     1561 gcggccgcgc ccttcggtcg gttcgtcgtc gttcggctcg accgcgtcgt cgggctcgaa
     1621 gtcgcgtgat ttacgtgcca tgagagcgac gagttatcac gcgaggggcg gcggcgcaac
     1681 cagcgcggcg caggcggccg cgcgcgccgt catcagcgca cgctcgcgct cgttgctcgt
     1741 caacgaagcc gcgcgctcga attccaaacg cgcctcggcg tgacgaccga gcttcgccag
     1801 aaaatcgccg cgcacggcgg gcagcaagtg atacgacttc aaggcaccgc cagcgacgag
     1861 ctcgtcgacg atgctaaggc cttccgcggg accgaacgcc atggccacgg cgacagcgcg
     1921 attcagctca acgaccggcg acggcgtcag cgccgcgagc gcgtcgtaca acgcgacgat
     1981 acgcggccag tccgtgtccg cggccgtcgc agcccgcgca ttacaggcgg cgatcgcggc
     2041 ttgcagcgcg tacggtccca gcgccccgcc gagcgcctcg gcgcgctcga gcgcggccag
     2101 cccgcgccgg atcaacaacc gatcccaacg gccgcggtcc tgctcgagca gcaacaccgg
     2161 ctcgccgttg gcggcggtcc gcgccgcgct gcgcgacgct tggatctcga gcagcgcgac
     2221 gaggccatgc acctcgggct cgcgcggcgc aagctcggcc agcatgcgcg cgagcttcag
     2281 cgcatcgtcg cagagcgccg gccgcaacca atcgtcgccg gccgtcgccg agtacccttc
     2341 gttgaacacg agatagacga cctcgagcac cgaagcgagc cgctccgaga gctcctcgcc
     2401 gcgcggcact tcgaacggca cgcgcgcctc ggccagcgtg cgcttcgcgc gcacgatgcg
     2461 ctgcgcgatg gtcggctcgg ggacgaggaa cgcgcgcgcg atctccgtcg tcgtcagtcc
     2521 gccgaccatg cgcaaggtca gagcgacgcg cgcttccatc gcgagcacgg gatggcacgc
     2581 gatgaacatc aggctcagca tgtcgtcacc gatttgatcg tcgagcgctt cgtcgatggc
     2641 ctcgcgttcg tcgctcgctt cgccgcccat ggcctcgagc tcgccgccga gctcctcgtg
     2701 cttgcggcgc acgagcttcg cgtggcgcac ggcgtcgatc gcacggtgct tcgccgcctg
     2761 catgagccac gcgcccggct tgaccggaat gccctcctcg ggccactgct ctagcgccgc
     2821 gacgagcgct tcctgcgcga gctcctcggc tctgccgacg tcgcgcacga cgcgcgtgag
     2881 cccggcgatg agcttcggcc actcgatgcg aaacaccgct tcgatcgcgg catgcgtcgc
     2941 cgaaccgctc attggccgaa agtgctccaa cccgcgacgg gccggatgtc gagctcgacc
     3001 tcgccgagca cggcggcata gcgctgcgcc cactcgatcg cttcggcgcg cgactcgagg
     3061 tcgacgatcg caaagccgcc gacgagctcc ttcgattcgg cgaacggacc gtcgacgacg
     3121 gtttgcttgc cgccgaagtt gcgtag
//
LOCUS       DATLEE010000043         2476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2280827, whole genome shotgun sequence.
ACCESSION   DATLEE010000043 DATLEE010000000
VERSION     DATLEE010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2280827"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VM692_00910"
     CDS             complement(<1..888)
                     /locus_tag="VM692_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50747.1"
                     /translation="MATTPTMLERERSHARLAAYWLPACTLALAPVAYADLLFEPRVA
                     VGVIHTDNLTLDSGDDPQAETVYEVVPSFRFEQESPRVSSSANYRVEGYRYSSLDADE
                     VYNILYGRLRVEPDPDNFFLDLGASRDQAIRDLTAPIATSNLPISTNRMDRDEAYFGP
                     SFTYPIGDNVTAGGQFRRTRVRYDEPETGFAFARDFDVDTTEFSLDNYQKELGFTWAT
                     RYSAEKTEYAFFPSYEYRQAAVEIGAWLGQRVRLFGTKGKESAWDTPFDPSLEDDFWE
                     VGVATRDNDRFTAEVATGER"
     gene            complement(857..1726)
                     /locus_tag="VM692_00915"
     CDS             complement(857..1726)
                     /locus_tag="VM692_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HUQ50748.1"
                     /translation="MSRIEDALKKLRQPSTPVAPPAKTTQRSSIPLASKKKVAVEGDK
                     HHVVQADLVAGGLLAPLDHATAVADDFRRIKRPLIVNATKADAELADAMNVIMIASAL
                     PGAGKTFCSVNLAVSISLERELNVLLVDADVPKPHISREFGLSDKPGLIDLLLDEQSD
                     VADFLVRTDLNDITVLPAGRAHPQATELLASDRMSQIVRELASRYRDRIVLLDSPPLL
                     LTSEAQALASQVGQIALVVEAGETSHQMLAHALEVLDHDKAVNLILNKTRGVMGGGYY
                     GGDYGYYADDARA"
     gene            complement(1723..>2476)
                     /locus_tag="VM692_00920"
     CDS             complement(1723..>2476)
                     /locus_tag="VM692_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50749.1"
                     /translation="TELHPRVVSTQETIAHLRKECEAAAQAAPGVDIGASLSTSESLE
                     LNPVYQNLKIQFNSAEVDLAEARAQLATYQASVNDLRRDVDKITEVEAQLKQLNRDYD
                     VIQGRHQELTKRWEDLMATKRLDPVTDNVEFRRIEPPFALIDPVGPNRPLLLAAVLAV
                     ALGAGLVVAFALNQLRPVYFTRMRLAKATQFPILGSISMILSPAAIANRRAKALVWGG
                     ACLVLVLSCALAVVFAQPTSAWLRAITGGVVI"
BASE COUNT          453 a          768 c          779 g          476 t
ORIGIN      
        1 ccgctcgcct gtcgcgacct cagccgtgaa gcggtcgtta tcgcgcgtcg cgacacccac
       61 ttcccaaaag tcgtcttcga gcgaggggtc gaacggcgtg tcccaggcgc tctccttacc
      121 tttcgtgcca aagaggcgga cgcgctgccc gagccaagct ccgatctcga cggcggcctg
      181 ccgatactcg tagctcggga aaaaggcgta ctcggtcttt tccgccgagt aacgcgtcgc
      241 ccaagtgaag ccgagctctt tttgatagtt gtccagcgag aactcggtcg tgtcgacgtc
      301 gaaatcgcgg gcgaacgcaa agccggtctc gggttcatcg tagcgaacgc gcgtgcgacg
      361 gaactggccg ccggcggtca cattgtcccc gatcgggtac gtgaacgacg gcccgaagta
      421 ggcctcgtct cgatccatac gattggtcga gatcggtagg ttcgacgtcg ctatgggggc
      481 ggtcagatcg cgaattgcct gatctcggct ggccccaagg tccagaaaaa agttgtcagg
      541 atccggctcg acgcgcaacc ggccgtaaag aatgttgtag acctcgtccg cgtcgaggct
      601 gctatagcga tagccttcga cgcgatagtt ggcgctcgag ctgacacgcg gactttcctg
      661 ctcgaatcgg aacgacggaa ccacctcgta gaccgtctcg gcctgggggt cgtcgccgga
      721 atcgagcgtc aggttgtccg tatggatgac gccgacggca actcttggct cgaacagcaa
      781 gtcggcgtat gcgaccggcg ccagtgcgag tgtgcaagcc ggcaaccagt aggccgcaag
      841 gcgtgcatga ctgcgttcac gctcgagcat cgtcggcgta gtagccatag tcgccgccgt
      901 agtatccgcc gcccattacg ccgcgggtct tgttcaatat gaggttgact gccttatcgt
      961 ggtcgagaac ctcgagcgcg tgcgccagca tctgatgcga cgtctctccc gcctcgacga
     1021 cgagcgcgat ctggcccacc tggctcgcga gcgcctgcgc ttcgctcgtc aacaacaacg
     1081 gcggcgagtc gagcagcacg atgcgatcgc gatagcggct cgcaagctcg cgcacgatct
     1141 gcgacatgcg atcgcttgca agcagctccg tagcctgcgg gtgtgcccgt ccagccggca
     1201 aaacggtgat gtcgttcaga tccgtgcgca cgaggaaatc cgcgacatcg ctttgctcgt
     1261 caaggagcag gtcgatcaaa cccggtttgt cgctcaaacc gaactcccgg ctgatatgcg
     1321 gcttcggaac gtcggcatcg acgagcagaa cgttgagctc tcgctccagc gagatgctga
     1381 cggccagatt gaccgagcaa aatgtcttac ccgcgcccgg cagcgcgctc gcgatcatga
     1441 tgacattcat cgcgtcggcg agctcggcat cggcttttgt agcgttgacg atcagcggcc
     1501 gcttgattcg gcgaaagtcg tcggccaccg ccgtcgcgtg atcgagcggc gcaagcaagc
     1561 cgcccgccac caagtcggcc tgaacgacat gatgcttgtc gccctcgact gcaaccttct
     1621 tcttactcgc gagcggaatg gacgagcgct gcgtcgtttt ggccggcggc gcaaccgggg
     1681 tgctgggctg ccgcagtttt ttgagggcgt cctcgatcct gctcatatga cgactccgcc
     1741 cgtgatcgct ctgagccagg cggatgtagg ctgcgcgaat acgacggcta gcgcgcagga
     1801 gagcaccaat acgagacagg ctccgcccca aacgagtgcc ttggcacgcc ggttcgcgat
     1861 tgccgcaggg ctcagaatca tcgagatcga gcccaagatc gggaactgcg tggccttcgc
     1921 caagcgcatc cgcgtgaagt acacggggcg tagctgattt agcgcgaagg caaccacgag
     1981 cccggcgccg agcgccaccg ccagcacggc cgcaagcagc agcgggcgat tcggaccgac
     2041 gggatcgata agcgcgaacg gcggctcgat gcgccgaaat tccacgttgt cggtcacggg
     2101 gtcgagccgc ttcgttgcca tgaggtcttc ccagcgcttc gtcagctcct ggtgacgccc
     2161 ctggatcacg tcgtagtcgc ggttgagctg cttcaactga gcctcgacct cggtaatctt
     2221 gtcgacgtct cggcgcagat cgttgacgga tgcttgatag gtggccagtt gcgcgcgcgc
     2281 ctcagcgagg tcgacctcgg cgctgttgaa ttggatcttc agattctgat agaccgggtt
     2341 caattccaga gattcgcttg tgctcagcga tgcaccgatg tcgacgccgg gcgctgcctg
     2401 cgccgcggcc tcacattctt ttctgaggtg agcgatggtt tcctgcgtcg acacgacgcg
     2461 ggggtgtagc tccgtg
//
LOCUS       DATLEE010000044         2893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4516173, whole genome shotgun sequence.
ACCESSION   DATLEE010000044 DATLEE010000000
VERSION     DATLEE010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4516173"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VM692_00925"
     CDS             complement(<1..544)
                     /locus_tag="VM692_00925"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ50750.1"
                     /translation="MTLRSGSIIAAALLAVPAAAHHGIGTFDTRTEITVSGNVTGIDF
                     VNPHAYLYLEATGAGGKRAAWRCEMRSATTLRRSGWSPEMFKPGQPVTITGAPDRNDP
                     SSCYLSTIVFANGTSADRYGQLSTAPAPRTAGAQRAARLPSGAPNLSGDWAPEQLVMT
                     DPRGRGGALVPLSQAGSFEPG"
     gene            673..2172
                     /locus_tag="VM692_00930"
     CDS             673..2172
                     /locus_tag="VM692_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50751.1"
                     /translation="MNTGRIVCRLLLATFAAVAPLATALPQSAPGAGYEVEKSQSVEN
                     APAGSVGRKTTDREHRVGNAEDTLGNEYTYMLTFGGFARRCPTSAGIVTGDFEYSIVY
                     DGMETGDDGVIRREHHARRLVAQLEGHVGDDAKLMYVQLTGAFTIERSGTDVSPTSER
                     RPVEKRFTPGPAGEPDFPAMSAAVEMTADIAVASVILVAGTLYRTAEAEWQKINECVE
                     FSFDPPTDTEQLGPSQSRQVRSEVRSKADGAVVPWTSDNINAIRGIGTVSPRTIEIGS
                     TDPVTYTASTRPRRGHGIDIGATSKAGVANGFWRIVDRYEGSFTQVDKTDGTVGILSA
                     ADVQTLTANLVWTSDDAGQQAPSFGDTPSTFYRPTQGDFTIEVSNVTNNSAGGKCQTQ
                     GRHTFALSELPPSKLQYFLLEIAADGRYKLMLGMPDYPQLTFDVDAVCEFRGGRTARQ
                     RVPAMLPAVVIGIQQGTLNDEGAVIGEMTPQRRGPLTTTGNWTLKLPQP"
     gene            2210..2476
                     /locus_tag="VM692_00935"
     CDS             2210..2476
                     /locus_tag="VM692_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HUQ50752.1"
                     /translation="MCRNIKTLFNFEPPATEAEIRASALQFVRKLSGFNKPSKANEEA
                     FNRAVVEVTSAASRLIFSLETTAEPRDREVEADKAKERAKARFG"
     gene            complement(2473..>2893)
                     /locus_tag="VM692_00940"
     CDS             complement(2473..>2893)
                     /locus_tag="VM692_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUQ50753.1"
                     /translation="APRASFPTFWTMLPVRAPLLAAWAAGAAAARLAGASEGKLVRTA
                     LACLTKLFAGSERCLADFQGAYLHDWQADPFACGAYSYLVAGGGNARELLARPLKNTL
                     FFAGEAAETGGESGTVAGALESGKRAAQQLLAPLARR"
BASE COUNT          536 a          947 c          967 g          443 t
ORIGIN      
        1 cacccggctc gaagctgccg gcttgtgata gcggaacgag ggcgccgccg cggccgcgcg
       61 gatccgtcat cacgagctgc tcgggcgccc agtcgccgga caaattcggc gcgcccgatg
      121 gcaggcgcgc tgcacgctgg gcgccggccg tgcgcggcgc cggcgccgtg gacagctggc
      181 cgtagcgatc ggccgaggtg ccgttcgcaa agacaatcgt cgagaggtag caggagctcg
      241 gatcgttgcg atccggcgcg cccgtgatcg tgacgggctg gccgggcttg aacatctcgg
      301 gcgaccagcc ggagcggcgc agcgtcgtcg cggagcgcat ctcgcagcgc cacgcggcgc
      361 gtttgccgcc ggcgcccgtc gcttcgagat agaggtacgc atgcggattc acgaaatcga
      421 ttccggtcac gttgccgctg accgtgatct ctgttcgggt gtcgaaagtc ccgatgccat
      481 gatgcgcagc cgcgggtacc gcaagcagcg cggcggcgat gatcgagccg gatcgaagcg
      541 tcataactac ccccgaagag caatgcatgc gaacgcagcc atcgtaacaa tagcgccaac
      601 acgcggtaac tcggtgcgcg ttgaaaggcc tcccgttgac gcatacgctc tgcgtgttga
      661 cgggggacca gagtgaacac tggacgcatt gtgtgtcggc tcttgctcgc gacctttgcc
      721 gcggttgcgc cgctcgcgac ggcactgccg cagagcgctc ccggcgcagg gtacgaagtc
      781 gagaagagtc agtccgtcga aaatgcgccc gcaggctccg tcggccgcaa gacgacggac
      841 cgcgagcacc gcgtcggcaa cgccgaagac acactcggca acgaatacac gtatatgttg
      901 accttcggcg ggttcgcacg ccgttgtccg acgtccgcag gcatcgtcac gggcgacttc
      961 gagtattcga tcgtctacga cggcatggaa accggcgacg acggcgttat ccggcgcgag
     1021 catcacgctc gccggctcgt tgcgcaactc gagggtcatg tcggcgacga cgcgaagctg
     1081 atgtatgtcc agttgacggg cgcattcacg atcgagcgca gcggtacaga cgtttcgccg
     1141 acgtcggagc gccgcccggt tgagaagcga ttcacgccgg gcccggccgg cgagccggat
     1201 tttcccgcaa tgagcgcggc agtcgagatg acggccgata tcgccgtcgc gagcgtgatt
     1261 ctcgtcgccg gcacgctgta ccgtaccgcc gaggcggagt ggcagaaaat caacgagtgc
     1321 gtcgaattct cgttcgaccc gccgaccgat accgagcaac tcgggccgag ccagtcgcgg
     1381 caggttcgca gtgaggtacg cagcaaggcc gacggagcgg tcgtgccctg gacgtcggac
     1441 aacatcaatg ccatccgcgg catcggcaca gtctcgccgc gaacgatcga aattggctcg
     1501 acggacccgg tgacgtatac ggcatcgacg cggccgcgac gtgggcacgg aatcgacatc
     1561 ggagcgacgt ccaaggccgg cgtcgccaac gggttctggc gcatcgtcga ccgctacgaa
     1621 ggcagtttta cgcaagtgga caagaccgac ggaacggtcg gcattctgag cgccgccgac
     1681 gtccaaacgc tgacggccaa cctcgtgtgg acatccgacg acgcgggcca gcaggcgcca
     1741 agcttcggcg acactccttc gacgttctat cggccgactc agggagactt cacgatcgag
     1801 gtctcgaacg tcacgaacaa ctccgctgga ggcaagtgtc aaacgcaagg ccgccatacg
     1861 ttcgcgctca gcgagctgcc gccgagcaag ctgcagtatt ttctgctcga gattgccgcg
     1921 gacggccgct acaagttgat gctcggaatg ccggactatc cgcagctgac gttcgacgtg
     1981 gacgccgtct gcgaatttcg cggcggtcgc acggcgcgcc agcgcgtgcc cgcgatgctg
     2041 ccggcagtcg tgatcggcat ccagcagggc acattgaacg atgagggggc cgtcatcggc
     2101 gagatgacgc cgcaacggcg cggcccgctt acgacgaccg gcaattggac gttgaagctg
     2161 cctcagccgt gagagcgtgc cttatcatgg ttgggcacgg aggaacgtca tgtgccgcaa
     2221 tatcaagacc ttgttcaact tcgagccgcc ggccaccgaa gcggagatcc gcgcctcggc
     2281 gctgcagttc gtgcgcaagc tctctggctt caacaagccg tcgaaggcca acgaagaggc
     2341 gttcaaccgc gccgtcgtcg aggtaacttc cgcggcttct agactgattt tctcgctcga
     2401 gacgacggcc gagccgcgcg accgcgaggt cgaagcagac aaagccaagg agcgcgcgaa
     2461 ggcgcgcttc ggctagcgcc gcgccagcgg agcgagcagc tgctgcgctg cgcgcttgcc
     2521 gctctcgagg gcgcccgcga cggtgccgga ctcgccgccc gtctcggccg cctcgccggc
     2581 gaagaacaac gtgttcttta acggccgcgc tagcagctcg cgcgcgttac ccccgccggc
     2641 cacgagatag ctgtaggcgc cgcacgcgaa cggatctgcc tgccaatcgt gcagatacgc
     2701 gccttggaag tcggcgaggc agcgctcgct gccggcgaat aacttcgtca gacacgcgag
     2761 cgccgtgcgc acgagcttgc cttcgcttgc gccggcaagc cgtgcggccg cagcgcccgc
     2821 ggcccacgct gccaacagcg gcgcgcgtac cggcagcatc gtccagaacg tgggaaacga
     2881 cgcgcgcggc gca
//
LOCUS       DATLEE010000045         7449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_46857, whole genome shotgun sequence.
ACCESSION   DATLEE010000045 DATLEE010000000
VERSION     DATLEE010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46857"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VM692_00945"
     CDS             complement(<1..453)
                     /locus_tag="VM692_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HUQ50754.1"
                     /translation="MKALVKSEARPGIWMKDIAVPTVGPNDVLIKIAKTAICGTDMHI
                     YNWDAWAQKTIPVPMAVGHEYYGRIVDMGSEVRGFAIGDRVSGEGHITCGFCRNCRAG
                     RRHLCRNTVGVGVDRQGCFAEYLAIPGSNAFKLPDAVTDDIASILDPFG"
     gene            complement(450..1634)
                     /locus_tag="VM692_00950"
     CDS             complement(450..1634)
                     /locus_tag="VM692_00950"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HUQ50755.1"
                     /translation="MDNAFYQRLDFELEQLRSQGLFKNERVISSPQGGEIRANGADVI
                     NLCANNYLGLANHPAIREAAHDALDRYGYGMASVRFICGTDVLHKQLEASLSRFLGTD
                     DTILYSSCWDANGGLFETLLDEQDTVISDALNHASIIDGIRLCKARRLRYANRDLGEL
                     EQHLKDTQSCRTRLIATDGVFSMDGSIAPLREICDLTERYGALVMVDDSHATGFVGAG
                     GRGTPEHCGVSGRIDFLTGTFGKALGGGSGGYTSGRAPAIAWLRQRSRPYLFSNSIPP
                     VVAAATLRALDIVEQTPQLRATLFAHARRFRAGLTDAGFRLKPGEHPIVPVMIGDAAL
                     AGRFAERLLEEGIYVIAFSFPVVPRGEARIRTQMSAGLSAAQLTRALAAFVKVGRELE
                     LL"
     gene            1683..2081
                     /locus_tag="VM692_00955"
     CDS             1683..2081
                     /locus_tag="VM692_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50756.1"
                     /translation="MRLLGTVYESHGDRLPFKDRSGFVGGPDIVPCGCSLDSVLLVDS
                     DGDSDVFGVTNYELLGPGYILEQLEFYSNDLLHGVGLDQHFDFDQAPTTFGRLLVTKA
                     FGGVSDAFQAAIARVRAFTEPSPGAGSCHA"
     gene            complement(2124..2663)
                     /locus_tag="VM692_00960"
     CDS             complement(2124..2663)
                     /locus_tag="VM692_00960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006514429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ50757.1"
                     /translation="MPELRLRPAVTADVVWLELWDYDPDVIACSTDDANATVAFADAD
                     WNEELAAQDEHSQYFIAELDGRPIGAMQICDPHLERSHYWGEIAPNLRAIDIWIGGPN
                     DRAQGHGTEMMRLALERCFADPRVTGIVIDPLASNEAAHRFYHRLGFKPVGRRQFGRD
                     DCLVHELTRRDWAAVKAQR"
     gene            complement(2678..5458)
                     /locus_tag="VM692_00965"
     CDS             complement(2678..5458)
                     /locus_tag="VM692_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ50758.1"
                     /translation="MQRSVLSLAIAGLLATAAAVPAAAQDQPIEEVIVTGSYIRRPSQ
                     FDSPSPLVSVTSEDVAATGANDVGRVLEDLTINTGSQNNPDAFTQNFTTGTSNINLRG
                     LGVSSTLVLINGRRQTQSAVTTDRGENFVDTSSLPPMIAFERIEILKDGATALYGSEA
                     VAGVANFITRDDFVGFDLELGLQGNDQHPQRDAELSGVWGVGEGDTHVLVAFSHLDRD
                     PLTTFERRLSGPTDDLSQAGNPGSFLVPSLPGNPAYRSAWTAAFDSTRTGVADFVEPQ
                     LGRPAVPGAQPPVFADQNCTAIAAQDPKIVPAIAASVPSPIGAIPIGLCQFDFGGFYS
                     IVPEEQRSSVFVEAKHEFGDTLEGRIEAHVADNEALRNNSPSFPFAAFPTVAATHPDN
                     PYGTDVRFIGRIIGAGGVPIQSVHNSDTYRLAASLAGTLNSTWGWEIALQHSENDFFV
                     SAPDVLVDRFNNAIRGFGGEGCNPTTGTRGVAPCVYFNPFGSALTGTGTRNSPALLNY
                     LVGFESFDAHSELTSLEGYATRELGELGGGYAGLAVGAQLRGDELTYDYDANANRDNF
                     LFLVGNPDFGDDRDVEAVFVEFALPFTERLNLQLAGRYESYGDGIDSTDPKATLLWRP
                     TLDFSVRASVGTSFRAPSLFQAFGTQTTLGELIDPNVGSPQFFPVRTQPNPAGAALSP
                     EEADVLNLGVSFSPGELWEIGVDYWSFDYTNVIIEQNAQAILNAAALGNAQARAQVIR
                     DPASGLLLRVDSFYANASSLETDGLDIRVSRKLELDGGSSVTVGADATYIMSYDLEDP
                     QAGAIEGAGRRNFANFGTSTPEWKANVFMAWQDDRNAINAFVRYIDSYVDDEVDIGQG
                     SAFFRSIGSQVTLDAQYTRTLRADRAPKLTFGAINLLNRDPPTVHTSGGYDSKVHDPR
                     GRMFYAKALFRF"
     gene            complement(5476..>7449)
                     /locus_tag="VM692_00970"
     CDS             complement(5476..>7449)
                     /locus_tag="VM692_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50759.1"
                     /translation="VSLIVGATLSGCDSGSSANPAPITFTPAPAPTGAGFVALYAPPV
                     DVVPYPIDLYNPTGTRLAVPVKITSPLSAALNTLDGFSTSAVISAPFNAPLDPASLIP
                     WNPASMAPSAASIIVLNATAGTPLVPGVHYTVRIATAAGSGNSVLEIVPLRPLTPRTR
                     YVFIVTNRVRSTAGVAAGADLVFGAVRDAHLAGLASVPNQPALSPLFPAITPLINTAV
                     GLGLPGNTVAVAWSMQTQSISDVLDTIASSATARPATLVAAGTTAQLGLGLPGIASIY
                     TGFMEVPYYGDPANPLTSFWVNNQQAPPNVQNATPVARVPNKRIPLLATLPNAGSGRT
                     EPASGWPVVIFQHGITLDRTVMFAMADSFAQAGFAVVAIDLPLHGIRSTSNPLYQGPN
                     SPFGNNERHFNLDNVGATGVFTPDGQIDNGWQILNVGNPLNARDHIRQAVSDTINLTR
                     TLPTLNFPDGDANPDLDGNRIHFVGISLGSIISGVFLGLDTSVKTATLSSPAGPWTAI
                     LTDPQAVDFGAPIRAALSAQGLPQGTVGFDNFVRDLQTVIDPVDPINYAAAATATHPL
                     HVIEVLGDTAVPNVPNDYIAQLWGLPTVRTTTAVAPPGTVRGIVRFNAGVHSSLFNPQ
                     PNATVTTEMQRQTVTYAASAGSAIQITNTSVVQ"
BASE COUNT         1194 a         2382 c         2602 g         1271 t
ORIGIN      
        1 gccgaacggg tcgagaatgc tcgcgatgtc gtcggtgacg gcgtcgggca gcttgaatgc
       61 gttgctgccg ggaatcgcga ggtattccgc gaagcagccc tggcggtcca cgccgactcc
      121 gaccgtgttg cggcacagat ggcgccggcc ggcgcggcag ttgcggcaga agccgcaggt
      181 aatgtgccct tccccagaga cgcggtcgcc aatggcgaac ccgcgcacct cgcttcccat
      241 gtcgacgatg cggccgtagt actcgtggcc gacggccatc ggcaccggaa tcgttttctg
      301 cgcccaggcg tcccagttgt agatgtgcat gtcggtgccg cagatcgccg tcttcgcgat
      361 cttgatcagc acgtcgttcg ggcccacggt cggcaccgcg atgtccttca tccagatgcc
      421 ggggcgcgct tcgctcttga cgagtgcttt catagcaatt cgagctcccg gcccactttg
      481 acgaaagcgg ctagggcgcg cgtgagctgc gctgcgctca agccggccga catttgcgtg
      541 cgaatgcgcg cttccccgcg gggcaccacg ggaaacgaga acgcgatgac gtagattcct
      601 tcctcgagca accgttcggc gaaacgcccc gcgagcgccg catcgccgat catgacgggc
      661 acgatcgggt gctcgccggg cttgagccga aaacccgcgt ccgtgaggcc ggcgcgaaag
      721 cgccgcgcgt gcgcgaacag cgttgcccgc agttgcggtg tctgctcgac gatgtcgagc
      781 gcgcgcagcg tcgctgcggc caccacgggc ggaatgctgt tcgaaaatag atagggccga
      841 gagcgttgtc tgagccaagc gatggccggc gcgcggccgc tcgtgtagcc gccgctgccg
      901 ccgccgagcg ccttgccgaa cgtgcccgtg aggaaatcga tgcgtcccga gacgccgcag
      961 tgctccggcg tgccgcgacc gccggcgccc acgaaacccg ttgcgtgcga gtcgtcgacc
     1021 atgacgagcg cgccgtaacg ttcggtgaga tcgcagatct cgcgtaacgg cgcgatgctg
     1081 ccgtccatcg agaacacgcc gtcggtcgcg atgaggcgcg tgcggcagct ctgcgtgtcc
     1141 ttgaggtgct gctcgagctc gccgagatcg cgattcgcgt accgaaggcg gcgcgctttg
     1201 cagaggcgga tgccgtcgat gatgctcgcg tgattcagcg catcggagat gacggtgtcc
     1261 tgctcgtcga gcagcgtctc gaacagccca ccgtttgcgt cccagcacga cgagtacagg
     1321 atcgtgtcgt ccgtgccgag aaagcgcgac aacgacgcct cgagctgctt gtgcaggacg
     1381 tccgtgccgc aaatgaagcg gaccgacgcc atgccgtagc cgtaacggtc gagcgcgtcg
     1441 tgcgccgctt cgcggatcgc cggatggttt gccaagccga ggtagttgtt cgcgcagagg
     1501 ttgatgacat cggcgccgtt ggcgcggatc tcgccgccct gcgggctcga gatcacgcgt
     1561 tcgtttttga acaggccctg cgagcgcagt tgctcgagct cgaagtccag gcgttggtag
     1621 aaggcgttgt ccacgcgtca ccaaaaagaa gggcgccaat gcaattatag acggcgcgtt
     1681 cggtgcggct cctcggcaca gtctacgaaa gtcacggcga tcggctgccg ttcaaggatc
     1741 gcagcggctt cgtcggcggc cccgacatcg tgccctgcgg ctgttccttg gactccgtgt
     1801 tgctcgtcga ctccgacggg gacagcgacg tgttcggcgt cacgaactac gagctcctag
     1861 ggccgggcta catacttgag caactcgaat tttatagtaa cgatctgctg cacggcgtcg
     1921 ggttggatca gcacttcgac ttcgatcagg cgccgacgac gttcggtcgg ctgctcgtca
     1981 cgaaggcttt cggcggcgta tcggacgcgt ttcaagcggc catcgcgcgc gttcgcgcgt
     2041 tcacggagcc gtcgcccggc gccggaagct gccacgcata acgggcgagc ccgccggcgg
     2101 ccgttgtcgc tcgcggcggg cttctagcgc tgcgccttca cggcggccca gtcgcgccgc
     2161 gtcagctcgt gcacgaggca gtcgtcgcgg ccgaactgac gccggccgac cggcttgaag
     2221 ccgagcctgt gatagaaccg gtgcgctgcc tcgttcgaag ccagcggatc gatgacgatg
     2281 ccggtgacgc gcgggtccgc gaagcagcgc tcgagcgcga gcctcatcat ctcggtgccg
     2341 tggccttgag cgcgatcgtt ggggccgccg atccagatgt cgatcgcccg caagttcggc
     2401 gcgatctcgc cccagtaatg cgaccgctcg aggtgcggat cgcaaatctg cattgcgccg
     2461 atcggcctgc cgtcgagctc cgcgatgaag tactgcgagt gctcgtcttg agccgcgagc
     2521 tcttcgttcc agtccgcgtc ggcgaaggcg accgtggcgt tcgcgtcgtc cgtcgagcac
     2581 gcgatgacgt cggggtcgta gtcccagagc tcgagccaga cgacgtccgc cgtgacggcg
     2641 gggcgcaacc tcagctcggg cacgacatcc ccttacccta aaagcggaac agcgctttgg
     2701 cgtagaacat gcggccgcgc gggtcgtgca ccttggagtc gtagccgccg ctcgtgtgga
     2761 cggtcggtgg atccctgttc aacagattga tggcgccgaa cgtgagcttc ggcgcgcgat
     2821 ccgcgcgcag tgtccgcgtg tactgggcgt cgagcgtcac ctggctgccg atggagcgga
     2881 agaacgcgct gccctggccg atgtcgacct cgtcgtcgac gtaggagtcg atgtagcgaa
     2941 cgaaggcgtt gatcgcgttg cggtcgtctt gccaggccat gaacacgtta gccttccact
     3001 cgggcgtcga ggtgccgaag ttcgcgaaat tgcgccggcc cgcaccctcg atggccccgg
     3061 cctgcgggtc ctcgaggtcg tagctcatga tgtaagtggc gtcggcgccg acggtgaccg
     3121 agctgccgcc gtcgagctcg agcttgcgcg agacgcggat gtccaagccg tccgtctcga
     3181 gcgacgaggc gttcgcgtag aacgaatcga cgcgcagcag caagccactc gccggatcgc
     3241 ggatcacttg cgcgcgcgcc tgcgcgttgc ctagagccgc cgcattcaag atcgcctgcg
     3301 cgttctgctc gatgatgacg ttcgtgtagt cgaacgacca atagtcgacc ccgatctccc
     3361 acagctcgcc cggcgagaag ctgacgccta ggttcaagac gtcggcctcc tcgggcgaga
     3421 gcgcggcgcc ggccgggttc ggctgcgtgc gtaccggaaa gaactggggg ctgccgacgt
     3481 tgggatcgat gagctcgccg agcgtcgttt gcgtgccgaa ggcctggaac aacgacggcg
     3541 cgcggaacga cgtgccgacc gacgcgcgca ccgagaaatc caaggtcggg cgccacagca
     3601 gcgtggcctt cggatccgtc gagtcgatgc cgtcgccgta gctctcgtag cggccggcga
     3661 gctgcaggtt cagccgctcc gtgaacggca gcgcgaattc gacgaacacg gcctcgacgt
     3721 cgcggtcgtc gccgaagtca gggttgccga cgaggaacaa aaaattgtcg cggttggcat
     3781 tcgcgtcgta gtcgtacgtg agctcgtcgc cgcgcagctg cgcaccgacg gcgagaccgg
     3841 catagccgcc gccgagctcg ccgagctcgc gcgtcgcgta cccctcgagc gacgtgagct
     3901 cgctgtgggc atcgaagctc tcgaacccga ccaagtaatt gagcagcgca gggctattgc
     3961 gcgttcccgt ccccgtcagc gcgctgccga acggattgaa gtagacacac ggcgcaacgc
     4021 cgcgcgtgcc cgtggtcggg ttgcagccct cgccgccaaa gccgcgtatc gcgttgttga
     4081 accggtcgac gagcacgtcc ggcgcggaaa cgaaaaagtc gttctcgctg tgctgcagcg
     4141 caatctccca gccccacgtg ctgttcagcg tgcctgcgag cgatgccgcc aagcgatacg
     4201 tgtcgctgtt gtgcacgctc tgaatcggca cgccgccggc gccgatgatg cggccgatga
     4261 agcgtacgtc ggtcccgtac ggattgtcgg gatgcgtcgc ggcgaccgtt ggaaacgcag
     4321 cgaacgggaa tgacggcgag ttgttgcgca gcgcctcgtt gtcggcgaca tgcgcctcga
     4381 tgcggccctc gagcgtatcg ccgaactcgt gcttggcctc gacgaagacg ctgctgcgct
     4441 gctcttcggg cacgatcgag tagaagccgc cgaagtcgaa ctggcagagg ccgatcggaa
     4501 tcgcgccgat cgggctcggc accgacgccg cgatcgcggg cactatcttg gggtcctgcg
     4561 cggcgatcgc cgtgcaattc tgatccgcga acacgggcgg ttgcgcgccg ggcacggcgg
     4621 gacgcccgag ctgcggctca acgaagtccg cgacgccggt gcgggtgctg tcgaaggccg
     4681 ccgtccaagc ggaccggtag gcggggttgc ccggcaacga cggcacgagg aacgagccgg
     4741 gattgccggc ctggctcagg tcgtcggtcg ggcccgacag tcgccgctcg aaggtcgtca
     4801 gcgggtcgcg gtcgaggtga ctgaaggcga cgagcacgtg ggtgtcgcct tcgccgacgc
     4861 cccagacgcc ggaaagctcc gcgtcgcgct gtgggtgctg gtcgttgccc tgcaagccga
     4921 gctcgaggtc gaagccgacg aaatcgtcgc gcgtgatgaa gttcgcgacg ccggccacgg
     4981 cctcggagcc gtacagcgcc gtcgcgccgt ccttcaggat ctcgatgcgc tcgaaggcga
     5041 tcatcggcgg cagcgacgaa gtgtcgacga agttctcgcc gcgatccgtc gtcacggcgc
     5101 tttgcgtttg tcgcctgccg ttgatcagca cgagcgtcga gctgactccg aggccgcgca
     5161 gattgatgtt gctcgtgccc gtcgtgaagt tctgcgtgaa tgcgtcgggg ttgttctgcg
     5221 accccgtgtt gatcgtgagg tcctcgagca cgcggccgac gtcgttggcg cccgtggctg
     5281 cgacgtcctc gctcgtgacg ctgacgagcg gcgacggcga gtcgaactgc gacggccgcc
     5341 ggatgtacga gcccgtcacg atgacttctt cgattggctg gtcttgcgcc gcggccggga
     5401 cagcggccgc ggtcgcgagc agcccggcga tggcaaggga aagcactgat ctttgcatcg
     5461 gtctctccac ggctttcatt gcacgacgct cgtgttcgtt atctggatgg cgctaccggc
     5521 gctcgcggcg taggtcaccg tctgtcgctg catttccgtc gtcaccgtgg cgttcggctg
     5581 cgggttgaac agcgacgaat gcacgccggc attgaagcgc acgatgccgc gcaccgtgcc
     5641 cggcggcgca accgcagtcg tcgttcgcac ggtggggagc ccccagagct gcgcgatgta
     5701 atcgttcggc acgttcggca cggccgtgtc gccgagcacc tcgatgacgt gcagcggatg
     5761 cgtcgcggtc gcagccgccg cgtagttgat cggatcgacg gggtcgatga cggtctgcaa
     5821 gtcgcgcacg aagttgtcga acccgacagt gccttgcggc aagccttggg ccgacaacgc
     5881 ggcccggatc ggcgcgccga agtcgacggc ttgcgggtcg gtcaagatcg ccgtccaggg
     5941 cccggccggg ctcgacagcg tcgcggtctt gacgctcgtg tccaaaccca agaacacgcc
     6001 gctgatgatg ctgccgagcg agatgccgac gaaatgaatg cggttcccgt cgagatcggg
     6061 gttcgcatcg ccatcgggga agttcagcgt cggcagcgta cgcgtcaggt tgatcgtgtc
     6121 cgagacggct tggcgaatgt ggtcgcgcgc gttcagcggg ttaccgacgt tcaggatctg
     6181 ccagccgttg tcgatctggc cgtcgggcgt gaacacgccg gtggcgccga cgttatcgag
     6241 attgaagtgc cgctcgttgt tgccgaacgg gctgttcggc ccttgataca gcggattgct
     6301 cgtcgaccgg atgccgtgca gcggcaggtc gatcgccacg acggcgaacc ccgcctgcgc
     6361 gaacgagtcg gccatcgcaa acatgacggt gcgatcgagc gtaatgccgt gctggaagat
     6421 cacgacgggc cagcccgacg cgggctccgt gcggccgctg cccgcgttcg gcagcgtcgc
     6481 gagcaacggg atgcgcttgt tcggcacgcg cgcaacgggt gtcgcgttct gcacgttcgg
     6541 cggcgcctgc tgattgttga cccagaagct cgtcagcggg ttcgcggggt cgccgtagta
     6601 aggcacctcc atgaagcccg tgtagatgct cgcaatgccc ggcagcccga ggcccagttg
     6661 cgccgtcgtg ccggcggcta cgagcgtcgc cgggcgagcc gtcgccgacg acgcaatggt
     6721 gtcgaggacg tcactgatcg attgcgtttg catgctccaa gcgacggcga cggtgttgcc
     6781 tggaaggccg agcccgactg ccgtgttgat cagcggcgta atcgccggga acagcggcga
     6841 cagcgccggc tggttcggca cgctcgcgag gcctgcgagg tgggcgtcgc gcacggcgcc
     6901 gaacacgagg tccgcaccgg ccgcgactcc ggccgtgctg cgaacgcggt tcgtcacgat
     6961 gaaaacgtag cgcgtgcgcg gtgtcagtgg ccgcagcggc acgatctcga gcacgctgtt
     7021 gccgctgccg gccgcggtcg cgatgcgcac cgtgtagtgc actccgggta cgagcggcgt
     7081 gccggccgtc gcgttcagca cgatgatcga ggccgccgac ggcgccatcg aagcggggtt
     7141 ccacgggatc agcgacgcgg gatcgagcgg cgcattgaac ggcgcgctga tgacggcgga
     7201 cgtcgagaag ccgtcgagcg tgttcagcgc cgcgctcaac gggctcgtga tcttgaccgg
     7261 caccgcgagc cgcgtgcccg tcggattgta gagatcgatg gggtagggca cgacgtcgac
     7321 gggtggcgcg tagagcgcca cgaagccggc gccggtcggc gcgggcgcgg gtgtgaaggt
     7381 gatcggcgcc gggttcgcag aggagccgct gtcgcaaccg ctcaacgtcg cgccgacaat
     7441 cagagaaac
//
LOCUS       DATLEE010000046         2072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5265922, whole genome shotgun sequence.
ACCESSION   DATLEE010000046 DATLEE010000000
VERSION     DATLEE010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5265922"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VM692_00975"
     CDS             <1..457
                     /locus_tag="VM692_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008957349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HUQ50760.1"
                     /translation="EMMGFDKVGRLIERHVIEIAPLAFMRGRTLNESFVILDEAQNTT
                     PEQMKMFLTRIGFGSRAVVTGDITQTDLPRGKESGLKQVGRILQGVAGIEFIHFGAED
                     VVRHPLVQRIVSAYAREDADREPAGHEQAARREQDRREEDRREEDRDTE"
     gene            454..936
                     /gene="ybeY"
                     /locus_tag="VM692_00980"
     CDS             454..936
                     /gene="ybeY"
                     /locus_tag="VM692_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005763079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HUQ50761.1"
                     /translation="MTRRARAVVVTVQNAAARAQVPTSAQVRKWARTALADDVHGELT
                     VRIVGEGESADLNSRYRGKKGPTNVLSFRAEPPTAVAAEVEGSLPFGDVVICADVVER
                     EAREQGKPLAAHWAHMVVHGTLHLQGFDHERARDAAAMEACERALLAKLGFPDPYTIK
                     "
     gene            942..1799
                     /locus_tag="VM692_00985"
     CDS             942..1799
                     /locus_tag="VM692_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011866723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HUQ50762.1"
                     /translation="MSDDTHPSGDTGVSGWFQRLVQGRAGDVKTRDEIAEFLGGFKER
                     GIIGEDEFAMLQGVLETADTQVRDIMVPRSHMVVVAKDQTPEEVLKVIATSNHSRFPV
                     IGDDKDEVVGIVLAKDLLKHFVANKVPFDLQTFLRPAAFIPESKRLNTLLTEFRDSRN
                     HMAIVVDEYGGVSGLATIEDVLEQIVGEIDDELDPEAVSSIQPLDGGKFNVMALTRID
                     EFNEFFNTEVSDQDYDTVGGLVMHELGRLPRRGEQLDFAGLRFKVLRGDRRRLHTLEV
                     TRLPLPSAE"
     gene            1805..>2072
                     /locus_tag="VM692_00990"
     CDS             1805..>2072
                     /locus_tag="VM692_00990"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HUQ50763.1"
                     /translation="MNRPPVQYLLSILAGAVLPLAFAPYEQLWLAPLSYAVLLYLWRD
                     ATPRRAAGLAFAFGCASFGIGTYWTYIAVRIIGQALIFVGVCLTV"
BASE COUNT          382 a          654 c          694 g          342 t
ORIGIN      
        1 cgagatgatg ggcttcgaca aagtcggccg gctcatcgag cgccacgtca tcgagatcgc
       61 gccgctcgcg ttcatgcgcg gtcgcacgct gaacgagtcg ttcgtgattc tcgacgaagc
      121 gcagaacacg acgcccgagc agatgaagat gttcctgacg cgcatcggct tcggctcgcg
      181 tgccgtcgtg acgggcgaca tcacgcagac ggacttgccg cgcggcaagg agtcgggcct
      241 gaagcaggtc ggcagaatct tgcagggcgt ggccggcatc gagttcattc acttcggcgc
      301 cgaggacgtc gtgcgccatc cgctcgtgca gcgcatcgtg tcggcctatg cgcgcgaaga
      361 cgctgaccgc gagccggccg gccacgagca agcggcgcgt cgagagcagg accgccgaga
      421 agaggaccgc cgagaagagg accgagacac cgaatgacgc ggcgagcgcg cgccgtcgtc
      481 gtgacggtgc aaaatgcggc cgcgcgagcc caggtgccga cttccgcgca agtacggaaa
      541 tgggcacgca cggcgctggc ggacgacgtg cacggtgagc tgacggtgcg catcgtcggc
      601 gagggcgaaa gcgcggactt gaactcgcgc taccgcggca agaaggggcc cacgaacgtg
      661 ttgtcgtttc gagcagagcc gccgaccgcc gtcgccgccg aggtcgaagg atcgttaccg
      721 ttcggcgacg tcgtcatctg cgccgacgtc gtcgagcgcg aggcgcgcga gcaaggcaag
      781 ccgctcgcgg cgcattgggc gcacatggtc gtgcatggaa cgctgcactt gcagggcttc
      841 gatcacgagc gcgcgcgcga cgcggccgcg atggaagctt gcgagcgcgc attgttggct
      901 aagctgggct ttccggaccc ctacacgata aaatagttcc aatgtcggac gatactcacc
      961 ctagtggcga caccggcgta tccggatggt ttcagaggct cgtccaaggc agagccggtg
     1021 acgtcaaaac ccgcgacgag atagcggagt tcctcggagg gttcaaagag cgcggcatca
     1081 tcggcgaaga cgagttcgcg atgttgcaag gcgtgcttga aacggccgac acgcaggtcc
     1141 gcgatatcat ggtgccgcgc agccacatgg tcgtcgttgc gaaggatcaa actcccgaag
     1201 aagtgctgaa agtcatcgcg acgtcgaacc actcgcgctt ccccgtgatc ggcgacgaca
     1261 aggacgaggt cgtcggcatc gtgctcgcga aagatctgct gaagcacttc gtcgcgaata
     1321 aagtgccgtt cgacttgcag accttcctgc ggcccgccgc gttcatcccc gagagcaaac
     1381 ggctgaacac gctgttgacg gagttccgcg acagccgcaa tcacatggcc atcgtcgtcg
     1441 acgagtacgg cggcgtctcg gggcttgcga cgatcgagga cgtcctcgag caaatcgtcg
     1501 gggaaatcga cgacgagctc gatcccgaag cagtctcgtc gatccagccg ctcgacggcg
     1561 gcaagttcaa cgtcatggcg ctgacgcgca tcgacgagtt caacgaattc ttcaacaccg
     1621 aagtttcgga ccaggactat gacacggtcg gcggcctcgt catgcacgag ctcggccgct
     1681 tgccgcgccg cggcgagcag ctcgacttcg cggggctgcg cttcaaagtg ctgcgcggcg
     1741 atcgccgacg cctacacacg ctcgaagtca cgcgcctgcc gctgccgtcg gccgagtagc
     1801 gttcttgaat cgcccgccgg tgcagtacct gctgagcatc ctcgcaggtg cagtcctgcc
     1861 gctcgcgttt gcgccgtacg agcagctctg gctcgcgccg ctgtcgtacg ccgtgctgct
     1921 gtacctgtgg cgcgacgcga cgccgcggcg cgcggcgggg ctcgctttcg cgtttggctg
     1981 cgcgagcttc ggcatcggca cctactggac ctacatcgcc gtgcgcatca tcggccaggc
     2041 gctgattttc gtgggcgtct gtctgacggt cg
//
LOCUS       DATLEE010000047         3026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_50586, whole genome shotgun sequence.
ACCESSION   DATLEE010000047 DATLEE010000000
VERSION     DATLEE010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50586"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VM692_00995"
     CDS             <1..495
                     /locus_tag="VM692_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HUQ50764.1"
                     /translation="DALIIAGQTIAMSMGLGLATMVDPQRGISVPVISQFFVIMGFLI
                     FLALGGHLATLRLIADSFEILPLGQPLSPDGAWTIAAWGSQMFAGALRIALPAATALL
                     VANIAFGVMSRAAPTLNLFAVGLPAGLLIGFLLLLLNIGHLSALLAQFLDATLTMLAQ
                     ALAA"
     gene            511..1758
                     /gene="flhB"
                     /locus_tag="VM692_01000"
     CDS             511..1758
                     /gene="flhB"
                     /locus_tag="VM692_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HUQ50765.1"
                     /translation="MAENDTPQHDRTEQPSAKRLEDARARGEVPRSRELAMTAVVMAG
                     AAVLLGGGNYFAEGFAALFELGLKVPREALFDAQRLPNALLEGFGGGIRLLVPIAAAT
                     IIAAIVGSIALGGWSFSTTAMTPNFSRLNPITGLGRIASMNGLVELLKALAKFAIVAA
                     VAGMLLWRFGADFLALGTLTLEAAIGRAAWLIGVCLMGLAASLLLVAAADVPFQFWQH
                     RRQLRMTKQEAKDELKETEGRPEVRSRIRSLQRALATRRMMADVPKADLVAMNPSHYA
                     VALRYDAKTMKAPRVVAKGADLIAFQIRRVAEANKVPIFEHPQFARALYFTSDIGKEV
                     SPRLYVAVAQVLTYIYQLTGRSAKPGTSPRMPQRKGRPKMPVLAIDPELIEPKRGRAR
                     PRGVAGVPGSQDAHPRPRDGGKA"
     gene            1755..>3026
                     /locus_tag="VM692_01005"
     CDS             1755..>3026
                     /locus_tag="VM692_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HUQ50766.1"
                     /translation="MTSALMNRLADVGRMGLGVPLLVVALLAMVVVPLPPFLLDAFFT
                     FNITLSLVIMLAVIYVARPLDFAVFPTVLLGVTLLRLALNVASTRVVLLHGHTGTSAA
                     GRVIESFGEFVIGGNYAVGIVVFVILVVINFMVVTKGSGRVSEVTARFTLDAMPGKQM
                     AIDADLNAGLITQEQAVARRNEVAQEADFYGAMDGASKFVRGDAVAGILILAINVIGG
                     IVIGTLDHGLPIAEAGRVYTLLTIGDGLVAQIPALLSSTAVAVIVTRMSRASTLSEQV
                     VRQLVQHPRALTIAGGVLGIIGLLPGMPNVIFLLLAAATIGAGYWLEKRAKAEPVAAP
                     KADAAAKPEAPAAERELGWEDVSAIDLIGLEVGYRLIPLVDRNQSGPLLARIKAVRRK
                     LSEELGFLVQSVHIRDNLELKPNGYRISILGV"
BASE COUNT          458 a         1029 c         1001 g          538 t
ORIGIN      
        1 gacgcgctga tcattgccgg ccaaacgatc gccatgagca tgggcctcgg cctcgcgacg
       61 atggtcgacc cgcaacgcgg catctcggtg cccgtcatca gccagttctt cgtgatcatg
      121 ggtttcctga ttttcctggc gctcggcggc cacctcgcca cgctgcggct gatcgccgac
      181 agcttcgaga tcctgccgct cggtcaaccc ttgagccccg acggcgcctg gacgatcgcg
      241 gcatggggca gccagatgtt tgccggcgcg ctgcgtatcg cgttgccggc cgcgacagcg
      301 ctgctcgtcg cgaacattgc gttcggcgtc atgagccgcg cggcgccgac gttgaacctg
      361 ttcgccgtcg gactgccggc gggccttctg atcggtttcc tgttgctgct cttgaacatc
      421 ggccacttga gcgcgctgct cgcgcagttt ctcgacgcga cgttgacgat gctggcccaa
      481 gcgctggcgg cctgacgcga gcgacgaacc atggcagaga acgacacacc gcaacacgat
      541 cgcactgagc aaccgagtgc caagcgcctc gaagatgctc gtgcgcgcgg cgaggtgccg
      601 cgctcgcgcg agctcgcgat gacggcggtc gtcatggccg gtgcggccgt gctgctcggc
      661 ggcggcaatt acttcgcgga aggtttcgcc gcgctgttcg agctcggcct caaggtgccg
      721 cgcgaagcgc tgttcgacgc ccagcggctg ccaaacgcgc tgctcgaagg cttcggcggc
      781 ggcatccggc tgctggtgcc gatcgccgcc gcgacgatca ttgccgccat cgtgggctcg
      841 atcgcgctcg gcggctggtc gttcagcacg acggcgatga cgccgaactt ctcgcgcctt
      901 aatccgatca cgggtttggg ccgcatcgcg agcatgaacg ggctcgtcga gctcctgaag
      961 gcactcgcga agttcgcgat cgtcgcggcc gtcgccggca tgttgctgtg gcgcttcggc
     1021 gccgactttc tcgcgctcgg cacactgacg ctcgaggccg cgatcgggcg cgccgcgtgg
     1081 ctgatcggcg tctgcctgat ggggctcgcg gcgagcctgc tgctcgtagc cgccgcggac
     1141 gtgccgtttc agttctggca gcaccgccgc caactgcgca tgacgaagca ggaagcgaag
     1201 gacgagctca aagagaccga gggtcgcccc gaggtccgct cccgcattcg cagcttgcaa
     1261 cgcgctctcg cgacgcgtcg catgatggcc gacgtgccga aggccgatct cgtggccatg
     1321 aacccgtcgc actacgccgt ggcgctgcgc tacgacgcga agacgatgaa ggcgccgcgc
     1381 gtcgtcgcga aaggcgcgga cctcatcgcg ttccaaatcc ggcgcgtcgc cgaggcgaac
     1441 aaggtaccga tcttcgagca cccgcagttc gcgcgcgctc tgtatttcac gagcgatatc
     1501 ggcaaggaag tctcgccgcg gctctacgtc gccgttgcgc aagtactgac ctacatctat
     1561 cagctgacag gccggtcggc gaagccgggc acgtcgccgc gcatgcccca gcgcaagggc
     1621 cggccgaaaa tgcccgtgct cgcgatcgat cccgagctga tcgagccgaa gcgtggccgt
     1681 gcgcgaccgc gaggcgtggc gggagtgccg ggatcgcagg acgcacatcc ccgcccgcgt
     1741 gacggaggca aagcatgacg tcggccctca tgaaccgcct cgccgacgtc ggccgtatgg
     1801 gcctcggcgt gccgctgctc gtcgtcgcgc tgctcgcgat ggtcgtcgtg ccgctgccgc
     1861 cgttcctgct cgacgccttc ttcacgttca acatcacgct gtcgctcgtg atcatgctgg
     1921 ccgtcatcta cgtggcgcgg ccgctcgact tcgcggtgtt ccccaccgtg ttgctcggcg
     1981 tgacgctgtt gcgcctggcg ctgaacgtcg cttcgacgcg cgtcgtgctg ctgcacggcc
     2041 atacgggcac ctctgccgcg ggccgcgtca tcgagtcgtt cggtgagttc gtgatcggcg
     2101 gcaactacgc cgtcggcatc gtcgtgttcg tgatcttggt cgtcatcaac ttcatggtcg
     2161 tgacgaaggg ctcgggccgc gtctcggaag tcacggcgcg cttcacgttg gacgcgatgc
     2221 cgggcaagca aatggcgatc gatgccgact tgaacgccgg actcatcacg caggaacaag
     2281 ccgtcgcgcg gcgcaacgaa gttgcgcaag aggccgactt ctacggcgcg atggacggcg
     2341 cgagcaagtt cgtccgcggc gacgcggtcg ccggcattct gattcttgcg atcaacgtca
     2401 tcggcggcat cgtgatcggc accctcgatc acggcttgcc gattgcggaa gccggccgcg
     2461 tctatacgct gctgacgatc ggcgacggcc tcgtcgcgca aatccccgcg ttgctgtcgt
     2521 cgacggcggt cgccgtcatc gtgacgcgca tgtcgcgcgc ctcgacgctg tccgagcagg
     2581 tcgtcaggca gctcgtgcag catccgcgcg cgttgacgat cgccggcggt gtgctcggca
     2641 tcatcggctt gttgcccggc atgccgaacg tgatcttcct gctgctggcc gccgctacga
     2701 tcggcgccgg ctactggctc gagaaacgcg caaaagccga gcccgtggca gcgccgaaag
     2761 ccgacgcagc ggccaagccg gaagcgccgg ccgccgagcg cgagctcggc tgggaagacg
     2821 tcagcgcgat cgatttgatc ggcctcgagg tcggttaccg gctgatcccg ctcgtcgacc
     2881 gcaatcaatc gggcccctta ctcgcgcgca tcaaagccgt gcggcgcaag ctttccgagg
     2941 agctcggctt cctcgtgcaa tccgtgcaca tccgcgacaa cttggagctc aagccgaacg
     3001 gctatcgcat ctcgatcttg ggcgtg
//
LOCUS       DATLEE010000048         3379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8224693, whole genome shotgun sequence.
ACCESSION   DATLEE010000048 DATLEE010000000
VERSION     DATLEE010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8224693"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1483)
                     /locus_tag="VM692_01010"
     CDS             complement(8..1483)
                     /locus_tag="VM692_01010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HUQ50767.1"
                     /translation="MRALANTTAIRRRLLLTLFIPAVAVLAGGTVADYYATVRPLDQA
                     YDEALIDTAVAIAAHTKRSADGSPELTLPQDVLAVLRPDVRASTFFRVELADGTLVAG
                     DDDLPTPTSAANPTLAETTYRGARVRLVAFKTFADGERITVTTGETTAQRDDARQRAL
                     WTSIATDTIVVGIIVALIWLSVRLSLDPLREIEDEIKRRSARDLSPLEIERVPDEIRG
                     LIATLNRLFVTVGEHAGKQRQFLDNAAHQLRTPLAGIQAQLELMTADEPSPERQDRLR
                     RVLDAARRLSRTAQQLLMLARADESASLATELADVDLASIVESTIADYVTPAEAAGID
                     LGVDIAAAATRGIDWLIAEALRNLLGNALAHTPRGGSVTVSCGVWEGAPYLEVTDTGS
                     GIPAADRERIFGRFYRGSNARGSGSGLGLAIVKEVAGVHGASLSIDRGPGGIGTTVQI
                     VFGYTSPVAAETESPRTPPANAAEGPQPSLETVNAQSTPSR"
     gene            complement(1467..2135)
                     /locus_tag="VM692_01015"
     CDS             complement(1467..2135)
                     /locus_tag="VM692_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUQ50768.1"
                     /translation="MRVLVVEDDGLVGDAVKRAIEEAGFAVDLVGSAELARTALHAES
                     FDLVVLDIGLPRESGLRLLQGLRSRGETLPVLMLTARDALSDRVTALDLGADDYLTKP
                     FHVPELVARCRALIRRSHAVTTSQVTLGGLSIDLSHREVTAHGNRLALTEREWAILEC
                     FVLNAGRLVSKTRLMSAVADADDALTPNAIEQYVSRLRTKLGDAANIRAVRGMGYRLD
                     ESTG"
     gene            2396..>3379
                     /locus_tag="VM692_01020"
     CDS             2396..>3379
                     /locus_tag="VM692_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50769.1"
                     /translation="MRRWLALRRKALLAVAAIVVLLLLLTAVRDVARELRYEEVVAAM
                     KATTAAQIALAALATLLSYFALTGYDWAALRYARARVGYRIVAPTAFAAYALSNTVGF
                     GVLTGGAVRMRLYGAVGVDPAAISRAIAFGTVAFGIGTGSVAAVALLFDAATIAPLVH
                     VPGVLLRAAACVWLLLLVALLGACAARRAWPRSTAPGALPSLGLAGSQLLFSVADIVL
                     AAGALWWLLPPTQVGVLAFVGFFAIATLLGVVSHAPGGVGVFEAVMLVTLAPAVPAGQ
                     LAGALLLYRAIYFLAPLAIALVMFSAYELRPVARSPLGRAARGAAPLLLAAM"
BASE COUNT          451 a         1139 c         1211 g          578 t
ORIGIN      
        1 tccgcggtca gcgcgacggc gtgctctgcg cgttgaccgt ttccagcgac ggctgcggtc
       61 cctcggcagc gttggcgggc ggcgtgcgcg gcgactccgt ctccgcagca acaggactcg
      121 tgtagccgaa gacgatctgc accgtcgtgc cgatgccgcc gggcccgcgg tcgatcgaga
      181 gcgatgcgcc gtggacgccg gcgacctctt tgacgatggc gagccccaag ccgctgccgg
      241 agccgcgggc gttgctgccg cggtagaacc ggccgaagat ccgctcgcga tccgcggccg
      301 ggatgccgct gcccgtatcg gtgacctcga gatagggcgc cccttcccaa acgccgcagc
      361 tgacggtcac gctgccgccg cgaggagtat gggccagcgc gttgccgagc aagttgcgta
      421 gagcctcggc gatcagccaa tcgatgccgc gcgtcgctgc agcggcaatg tcgacgccga
      481 ggtcgatgcc ggccgcttcg gctggcgtaa cgtagtcggc gatcgtcgac tcgacgatcg
      541 acgcgaggtc gacgtcggcg agctcggtgg ccaggcttgc cgactcgtcg gcgcgcgcga
      601 gcatcagcag ctgttgcgcg gtgcggctca gacgccgcgc cgcgtcgagc acgcggcgta
      661 gccggtcttg ccgctcgggg ctcggctcgt cggccgtcat gagctcgagc tgcgcttgga
      721 tgccggccag cggcgtgcgc aattggtggg cggcgttgtc gaggaactgg cgctgcttgc
      781 cggcgtgctc gccgacagtg acgaacaggc ggttcagcgt cgcgatgagc ccgcgaatct
      841 cgtcgggcac gcgctcgatc tcaagcggcg aaaggtcgcg cgccgagcgg cgcttgatct
      901 cgtcttctat ctcgcgtaac gggtcgagcg acagccgtac gctgagccag atcaacgcca
      961 cgatgatgcc gacgacgatc gtgtcggtcg caatggaagt ccacaacgcg cgttgccgcg
     1021 cgtcgtcgcg ttgcgccgtc gtctcgcccg tcgtgacggt aatgcgctcg ccgtcggcga
     1081 acgtcttgaa ggcgaccagc cgcacgcgtg cgccgcggta cgtcgtctcc gcgagcgtcg
     1141 ggttggccgc ggacgtcggc gtcggcaggt cgtcgtcgcc ggcgaccaaa gtgccgtcgg
     1201 cgagctcgac gcgaaagaac gtcgaagcgc gaacgtcggg gcgcaacacg gcgaggacgt
     1261 cctgcggcag cgtcagctcg gggctgccgt cggccgagcg cttcgtgtgc gcggcgatcg
     1321 cgacggccgt gtcgatcaac gcttcgtcgt aggcttggtc gagcgggcgc acggtggcgt
     1381 agtaatcggc gacggtgccg ccggccagca cggccaccgc cggaatgaac agcgtcagca
     1441 gcaggcgccg ccgaatcgcc gtcgtattag ccagtgctct catcgaggcg gtaccccatg
     1501 ccgcgcaccg cgcggatgtt agcggcgtca ccgagcttgg tgcgcagtcg cgaaacgtac
     1561 tgttcgattg cgttcggcgt cagcgcgtcg tcggcgtccg cgacggcgct catgaggcgc
     1621 gtcttcgata cgagtcggcc ggcgttgagc acgaagcatt cgaggatggc ccattcgcgt
     1681 tccgtgagcg cgagccggtt gccatgtgcc gtgacctcgc gatgagacag atcgatcgac
     1741 aagccgccga gcgtcacctg cgaggtcgtc acggcgtgcg agcggcggat cagcgctcgg
     1801 cagcgcgcca cgagctcggg cacgtgaaac ggcttggtca agtagtcgtc ggcacccaag
     1861 tcgagcgccg tgacgcgatc ggagagggcg tcgcgagccg tcagcatcag cacgggtaac
     1921 gtctcgccgc gactgcgcaa tccctgcaac agccgcagcc cgctttcgcg cggcaagccg
     1981 atgtcgagca cgacgagatc gaacgactcc gcgtgcagag ccgtgcgggc gagctcggcg
     2041 gagccgacga ggtcgaccgc gaacccggcc tcttcgatgg cgcgtttgac cgcgtcgccg
     2101 acgagcccgt catcctccac gaccaacact ctcatctgcc tgcctcgtcg ccgtgccatg
     2161 tcgagcatgc gaccgttgcc tgacgccaag ctgacggcga gccggcctgt acggcctagg
     2221 acccgcggcg cggcgtgggt ttgggccccc tggcagtcag cgtcgtgtca gcttcgcaga
     2281 gcaaagttcc gtccgtaagg cgtcgaagtc aaatcggctc aaggcagcgg tttcgatggc
     2341 agctctctcg attaggaatc gtcaagaagc ggcgtcggtc gcggcgcttc gtggcgtgcg
     2401 ccgctggttg gcgctgcggc gtaaagcgct gctcgcggtc gccgcaatcg tcgtgctgct
     2461 gttgctgctt accgccgtgc gcgacgtcgc gcgcgagttg cgctacgagg aagtggtggc
     2521 cgcgatgaag gcgacgacag cggcgcagat tgcgctcgcc gcgctcgcga cgctgctttc
     2581 ctacttcgcg ctgaccggct acgactgggc ggcgctgcgt tacgcccgag ctcgagtcgg
     2641 ctaccgcatc gtcgcgccga cggcgtttgc ggcctacgcg ttgtccaaca ccgtcggctt
     2701 cggcgtgctg acgggtggcg ccgtccgcat gcggctctac ggcgccgtcg gcgtcgaccc
     2761 agccgccatc tcgcgcgcga tcgcgttcgg caccgtcgcg tttggaatag gcaccggatc
     2821 ggtggccgcc gtcgcgctac tcttcgacgc tgcgacgatc gcgccgctcg tgcatgtacc
     2881 gggcgtgttg ctgcgcgccg ccgcgtgcgt ctggctgctg ctgctcgttg cgctgctcgg
     2941 cgcttgcgct gctcgccgcg cgtggccgcg cagcacggcg ccgggcgcgt taccgtcgct
     3001 cgggctcgca ggctcgcaac tcttgttctc ggtcgcggac atcgtgctcg cggccggcgc
     3061 gctatggtgg ttgctgccgc cgacgcaggt cggagtgctg gctttcgtcg ggttcttcgc
     3121 gatcgctacg ttgctcggcg tcgtcagtca tgcgccgggc ggcgtcggcg tattcgaagc
     3181 cgtcatgctc gtgacgctgg cgccggcggt gccggccggc cagctcgcgg gtgcgttgct
     3241 gctctaccgc gcgatctact tcttggcgcc gctcgcgatc gcgctcgtga tgttctcggc
     3301 gtacgagctg cgacccgtgg cgcggtcgcc gctcggccgc gccgcgcgcg gcgccgcacc
     3361 gttgctgctg gccgcgatg
//
LOCUS       DATLEE010000049         2470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3782274, whole genome shotgun sequence.
ACCESSION   DATLEE010000049 DATLEE010000000
VERSION     DATLEE010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3782274"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..648
                     /locus_tag="VM692_01025"
     CDS             130..648
                     /locus_tag="VM692_01025"
                     /inference="COORDINATES: protein motif:HMM:NF027443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4833 domain-containing protein"
                     /protein_id="HUQ50770.1"
                     /translation="MACGIVAMLFAPAFSAIPAAAGSPDSVFHIDRNKNRNQVHYGVH
                     VDARCQPVGADPVYNYWLKREEDPPVVQPLKYFQQAAYGFQKQNIESDGRIEVRLRAL
                     PDRQLVVRVASVAGGCKAETFMTIDGKQAYLDKVFVFAEEGILLPTVRYIELYGRSND
                     GLAVYEKISVDQ"
     gene            652..1800
                     /gene="lptF"
                     /locus_tag="VM692_01030"
     CDS             652..1800
                     /gene="lptF"
                     /locus_tag="VM692_01030"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HUQ50771.1"
                     /translation="MPAGRTLERYLLREVGLAFLVGVALAVSVLFVLRIVEFVDLAFA
                     RGVPAKLVGALAIYIVPSYLEIALPMAALLAVVVVFARLGAEGELVAIRAAGIDLRRL
                     ARPLVVFSLVIATMSLVLGVWTRPWANRGIEATTYEMAKTRLTASLREGVFNTWFGGV
                     ILFVDELQAASGNMRNVMLAEEREEYGRKTIFASDGHVESNKDARTAYLKLSNGSLLT
                     YHPSGKYHDKTDFDSLELNLDMADEAGIDLANAGGPSAMSMERLLAERRARLARGETA
                     MEETIELHRKFVLPAAALLLPFVGLPLGSLGRRGVKSRGLLLSTMVVLVYYLLLTGAV
                     TVAREQILPVAVAMWIPDVLLALFAAALFQRVASERPVSLPLFRGARA"
     gene            1797..>2470
                     /locus_tag="VM692_01035"
     CDS             1797..>2470
                     /locus_tag="VM692_01035"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HUQ50772.1"
                     /translation="MNLLSRHLIDIYLRAFGLCMVGALILLFVVEFFSRIGDFASYDS
                     APSLVAAYFALKVPKWMVEVFPAASLLAVMLGIGSLVRSNEVQALQACGVSIARIGRP
                     LLLTSVVLSSLAMAWNEIVVPPTAARARVIKDIDIKGLATRGMLDATSLWLQVPEGFL
                     NIDYFDATNNVLDGVTLHGVDARFRLSSLIEIPGATWKNDHWEYEGGTVREFAPDGTF
                     TYSPLG"
BASE COUNT          390 a          820 c          797 g          463 t
ORIGIN      
        1 ttccgcaccc gaagatcgca cccgcgtgaa gtcggctgac cggtgggcta aggtgccggc
       61 atgcgttcgg ccacagcgct ctgccgctgc gcgatacgac acgccattcg aagcgcgacg
      121 gcgcgcgcaa tggcctgcgg aatcgtcgcc atgctttttg cgccggcgtt ttctgcgatc
      181 ccggccgcag cagggtcgcc cgactctgtc ttccacatcg accgcaacaa gaatcgcaac
      241 caggtgcact acggcgtgca tgtggacgca cgctgccagc ctgtcggcgc ggaccccgtc
      301 tacaactact ggctgaagcg cgaggaagac cctcccgtcg ttcagcccct caagtatttc
      361 cagcaagccg cctacggctt ccagaaacag aacatcgagt cggacggccg tattgaggtg
      421 aggctgcgcg ccctgcccga ccgtcagctc gtcgtgcgcg ttgcgtcggt ggccggaggc
      481 tgcaaggcgg agaccttcat gacgatcgat ggaaaacagg cctacctcga caaggtcttc
      541 gtgttcgccg aggagggcat tctgctgccc accgtgcgtt acatcgagtt atacggccgc
      601 agcaacgacg gcctcgccgt ctacgaaaag atctcggtgg accagtagct cgtgccggcc
      661 ggacgcactc tggaacgcta cctcctgcgc gaggtggggc tcgcttttct cgtcggcgtc
      721 gcgctggcgg tctcggtgct gttcgtgctg cgcatcgtcg agttcgtcga tctcgcgttc
      781 gcgcgtggcg tccccgcgaa gctcgtcggc gcactcgcca tctacatcgt tccttcgtat
      841 ctcgagatcg cgttgccgat ggccgcgctg ctcgccgtcg tcgtcgtctt cgcgcggctc
      901 ggcgcggaag gagagttggt cgccatccgc gcggccggca tcgaccttcg ccgtctcgcg
      961 cggccgctcg tcgtcttcag cctcgtgatc gcgacgatga gcctcgtgct cggagtgtgg
     1021 acgcggccgt gggccaaccg cggtatcgag gccacgacgt acgaaatggc gaagacgcgc
     1081 ctgacggcgt ccctgcgcga gggcgtgttc aacacctggt tcggcggcgt catcctgttc
     1141 gtggacgagc tccaggctgc gagcggcaac atgcgcaacg tcatgctcgc cgaagagcgc
     1201 gaggaatacg ggcgcaagac gatcttcgcg agcgacggcc acgtggagtc gaacaaggat
     1261 gcgcgcaccg cctacctgaa gctcagcaat ggaagcctcc tcacctacca cccgtcgggc
     1321 aagtaccacg acaagaccga cttcgattcc ctggagctca acctcgacat ggccgacgag
     1381 gccggcatcg acctcgcgaa tgcgggcgga ccgtcggcga tgagcatgga gcgactgctc
     1441 gccgaacgtc gggcgcggct cgcgcgcggc gagaccgcga tggaagaaac gatcgagctg
     1501 caccgcaagt tcgtgctgcc ggcagccgcg ctgctgttgc cgttcgtcgg attgccgctc
     1561 ggctctctcg gccggcgcgg cgtcaagtcg cgcggcctgc tgctctcgac catggtcgtg
     1621 ctcgtctatt acctgctgct gacgggagcc gtgacggtcg cgcgcgagca gattctgccc
     1681 gtcgcagtcg cgatgtggat tcccgacgtg ctgctcgcgt tgttcgcggc cgcgctcttt
     1741 cagcgcgtcg ccagcgagcg gccggtctcg ctgccgcttt tccggggggc gcgcgcatga
     1801 acctgctttc gcgccatctc atcgacatct atctgcgggc cttcggcctc tgcatggtcg
     1861 gcgcgctcat cctgctgttc gtcgtcgagt tcttcagccg catcggcgac tttgcttcgt
     1921 acgattcggc gccgtcgctg gtcgcggcct atttcgcgct caaagtcccg aagtggatgg
     1981 tcgaagtgtt tcccgcggcg tccctgctcg cagtcatgct cggcatcgga tctctagtgc
     2041 gcagcaatga agtacaggcg ctgcaggcct gcggcgtgag catcgcgcgc ataggcagac
     2101 cgctccttct tacgagcgtg gtgttgtcga gcctcgcgat ggcgtggaac gagatcgtgg
     2161 tacctcccac cgcggcgcgt gcgcgcgtca tcaaggacat cgacatcaag ggtctcgcga
     2221 cgcgcggcat gctcgatgcg acgtcgctgt ggctgcaggt gccggaaggc ttcctcaaca
     2281 tcgattactt cgacgcgacc aacaacgtgc tcgacggcgt cacgctgcac ggcgtggacg
     2341 cgcgcttccg gctgagcagc ctgatcgaga tacccggcgc gacgtggaag aacgatcact
     2401 gggagtacga aggcggcacc gtgcgcgagt tcgcgcccga cggaacattc acctactcgc
     2461 cgctcggcga
//
LOCUS       DATLEE010000050         5658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_58812, whole genome shotgun sequence.
ACCESSION   DATLEE010000050 DATLEE010000000
VERSION     DATLEE010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58812"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="VM692_01040"
     CDS             complement(<1..1182)
                     /locus_tag="VM692_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ50773.1"
                     /translation="MAISRASSLPAFCATLAFTPCIASAQPVTLAPAVADVPASPLAE
                     AAQRGDEQAVRELLAAGADANAPDRDGTPPLHWTVRVGDRATAERLVAAGADVNAASR
                     YGVTPLQLAIEAGDGAMARWLLDSGADATLADRAGEPPLLHAARAGNVDAAAALLDHG
                     VAVDVRDATFGQTALMLAAREGHLELAKLLLDAGANVDAATTAEEPPRFVPPSESPPG
                     LSRGIGIVRAGWPEGRGKRFPAGGGKTALLYATRGGHLDVVRLLVERGAKLELADDNG
                     VTPLLNAILNASIFRVSRTGTSAHLGVANALLDAGANVNAADWYGETPLWAAVDLRNL
                     ELGPADKATGIRDEAFALIERLLEAGAEPNARTREFPHERRYIVSVVGSVAWVDLTGQ
                     TP"
     gene            complement(1193..2476)
                     /locus_tag="VM692_01045"
     CDS             complement(1193..2476)
                     /locus_tag="VM692_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ50774.1"
                     /translation="MRYLTKKHLSRRTVLRGAGVALALPFLESMIPAGLRSASAAGVP
                     RARLACVYIPHGCVMDRWMPEAVGSGFAFTPTLRSLEPFRERLNVVSGLKLPAAYVGE
                     SSAAANHGRSSQCWLTCMPEDSGPSPTSADQLAAQHIGQQTPLPSLELALEAGSSISY
                     LTPQTPLPMETNPRVVFERLLGDGSTPEERAARQRQLSSLLDSVTGQVAALQRDLPAP
                     DRERMDRYLTDVRELERRLALAADSALATVDVPDKPNGIPADFEAHAMLMFDLLALAW
                     TADLTRIATFMVSRELSNRLYPRSGVNEGFHNASHHSGIAANIDRLAKLNEYHTRVGV
                     AYFLRKLSETQDGDGTLLDHSLIVYGSGMANPNQHDHDPLPMLLAGGGSGRLAGGRHV
                     RAADDTPFANLLVSVLDKLDVPVASFGDSTGALEI"
     gene            2626..3639
                     /locus_tag="VM692_01050"
     CDS             2626..3639
                     /locus_tag="VM692_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50775.1"
                     /translation="MKAFLAVLVSAASLPLLAQPPPSPPPPPCTPADNVVCGQNAPED
                     LVALGSQWVAAGAYAGSGGVVLIRTSDRASHTAYPAAGAANRHDKKTYPDCPGPPGQG
                     GAQFLTHGVYVEPGNGPVHKLFVVGHGARESIEVLQVDTSSAMPAVTWLGCVIAPDPI
                     GLNSVRGLPDGGFITTNFLPRGGTREATQQMMGGARNGELWEWHTATRWQKVPGSEAA
                     GANGVELSDDGKTLYVAAWGSQSFFRLARGAATPTRDEIPLGFRVDNIHWARDGTLWA
                     VGQATQSWKAVKVDPKTLAVREVLTQPDAPEFGAGTAVVEVGNKLWAGSFRGNRIVIL
                     PAP"
     gene            complement(3771..4151)
                     /locus_tag="VM692_01055"
     CDS             complement(3771..4151)
                     /locus_tag="VM692_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HUQ50776.1"
                     /translation="MPRTKVRSLLPVRKPRARRGVDENPALQAIWDVVAQLPPGSVST
                     YGDVARAAGLPGRARQAGYALKHTPDGLHLPWHRVVGAGGKIAFPSGSSAYREQLRRL
                     RSEGVKVVDGRVPRCTLLASPYGD"
     gene            complement(4164..>5658)
                     /locus_tag="VM692_01060"
     CDS             complement(4164..>5658)
                     /locus_tag="VM692_01060"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ50777.1"
                     /translation="GPQRITAALVDEQRCAGVKELYLGEVELGGAVMGLVVDGPYEPT
                     GAGETPSRRAIFDCQPQTAAAALPCAQQILTRLATRAYRRPIGAGSPEAKLLLEFYAQ
                     GRAEGGDFEVGIQYALSRLLVDPNFLYRFEHEPSGLATGDVYAISDLELASRLSFFLW
                     SSIPDDELLAVAVDGRLHEPATLAAQVERLLADDKAQRFVESFAGQWLKLRELDEVPS
                     QDPEFDADLRAAFRAETELVFGEVLRERRSVVELLDADYTYLNERLAAHYGIDGVRDS
                     YMRRVELPPESPRRGLLGHGSILTATSAPNRTSPVVRGQWIVQNILGAAVPTPPPGVE
                     ADLAKEASEAEGLKGDTVRERLEMHRANPTCAACHSIMDPLGLALENFDLVGRWREQE
                     DGHPIDATAELTDGTKLGGAADLRRALLSRSDAFVTSLTERLLTYALGRELEYYDRPA
                     VRGIVRAAAADGTTLAALIQAIVASEPFLKRVRTGDVAATAVAQAAR"
BASE COUNT          849 a         1962 c         2017 g          830 t
ORIGIN      
        1 cggcgtttgg ccggtcagat cgacccaagc gacggagccg acgacggaga cgatgtagcg
       61 gcgctcgtgc ggaaactcgc gcgtgcgtgc gttcggttcg gcgccggcct cgagcaggcg
      121 ctcgatcagc gcgaacgcct cgtcgcgaat gccggtcgct ttgtcggcag ggccgagctc
      181 gagattgcgc aggtcgacgg cggcccagag cggcgtctcg ccataccagt ccgccgcgtt
      241 gacgttcgcg cctgcatcga gcagtgcatt cgcgacgccc aagtgcgcgc tcgtgcccgt
      301 gcggctgacg cggaagatgc tcgcgttcaa gatcgcgttc agcagcggcg tgacgccgtt
      361 gtcgtccgcg agctcgagct tggcgccgcg ctcgacgagc aagcggacga cgtcgaggtg
      421 cccgccgcga gtcgcgtata ggagcgcagt cttgccgccg cccgcgggaa agcgcttgcc
      481 gcggccttcg ggccaaccgg cgcgcacgat gccgatgccg cgcgagaggc ccggcggcga
      541 ttcgctgggc ggcacgaagc gcggcggctc ttcggccgtc gtggcggcgt cgacgttcgc
      601 gccggcgtcg agcaacagtt tcgcgagctc gagatgccct tcgcgcgctg cgagcatcag
      661 cgccgtctgg ccgaaggtcg cgtcgcggac gtcgacggca acgccgtggt cgagcagcgc
      721 cgcggcggcg tcgacattgc cggcccgcgc ggcgtgcagc agcggcggct cgccggcgcg
      781 atcggcgagc gtcgcgtcgg cgcccgagtc gagcagccag cgcgccatcg cgccgtcgcc
      841 ggcttcgatc gcgagctgca gcggcgtaac gccgtagcgg cttgcggcgt tgacgtcggc
      901 gccggccgcg acgagccgct cggcagtcgc acggtcgccg acgcggactg tccagtgcag
      961 cggcggcgtg ccgtcgcggt cgggcgcgtt ggcgtcggcg cccgcggcca gcagctcgcg
     1021 gacggcttgc tcgtcgccgc gttgcgccgc ttcggcgagc ggcgaggccg gcacatccgc
     1081 gacggccggc gcgagcgtca cgggttgcgc gctcgcaatg caaggagtga acgccagcgt
     1141 cgcacagaag gcaggcagcg acgaagcgcg cgagatggcc atgccgcgcc cgtcagatct
     1201 cgagagcgcc cgtgctgtcg ccgaacgagg cgaccggcac gtcgagcttg tcgagcacgc
     1261 tgaccaagag attcgcgaac ggcgtgtcgt cggccgcgcg aacgtggcgc ccgccggcga
     1321 gccgccccga accgccgccc gcgagcagca tcggcagcgg gtcgtgatcg tgctgattcg
     1381 ggttcgccat gccgctgccg tagacgatca gcgagtggtc gagcaacgtg ccgtcgccgt
     1441 cttgggtctc gctgagcttg cgcaaaaaat aggctacgcc tacgcgcgtg tggtactcgt
     1501 tgagcttcgc gagccggtcg atgtttgccg cgatgcccga atgatgcgac gcgttgtgga
     1561 agccctcgtt gacgccgctg cgcggataca agcgattgct gagctcgcgc gagaccatga
     1621 acgtcgcgat gcgcgtgagg tcggccgtcc acgcgagggc cagcaagtcg aacatcaaca
     1681 tcgcgtgcgc ttcaaaatcg gccgggatgc cgttcggctt gtcgggaacg tcgacggtcg
     1741 cgagcgcgga atcggcggct agcgcgagtc gccgctcgag ctcgcgcacg tccgttaagt
     1801 agcgatccat gcgctcgcga tccggggccg gtaaatcgcg ctgcagcgca gcgacttgac
     1861 ccgtcacgga gtcgagcagg ctcgagagct ggcgctgccg cgcggcacgc tcctcgggag
     1921 tgctgccgtc gccgagcagg cgctcgaaca cgacgcgcgg attcgtctcc atcggcagcg
     1981 gcgtctgcgg cgtcagatac gagatcgacg agcccgcctc gagcgcgagc tcgagcgacg
     2041 gcagcggcgt ctgctggccg atgtgttgcg cggcgagctg gtcggccgac gtcggcgacg
     2101 ggccggagtc ctcgggcatg cacgtgagcc agcattgcga cgagcggcca tgattcgcgg
     2161 ccgccgacga ctcgccgacg taggccgccg gtagcttcaa gccgctgacc acgttcaacc
     2221 gctcccggaa cggctcgagc gagcgcagcg tcggcgtgaa agcgaagccg ctgcccacgg
     2281 cttccggcat ccagcggtcc atgacgcagc cgtgcggaat gtacacgcag gcgagccgtg
     2341 cgcgcggcac gccggccgcg gaggcgctgc gcagcccggc agggatcatg gactccaaga
     2401 acggcaacgc gagcgcgaca ccggcgccgc gcagcacggt gcggcgagac aggtgcttct
     2461 tcgtcaggta ccgcatggac cgcccccggg agctcgaacc aggttactcg gattcgcgag
     2521 atggcgacaa tttcatgagc gtcgctcgcg agggcccgaa agttgaccgg ctggccgctt
     2581 cgctctacgg tgacttgcgc cgtcgccttc gaggggggaa ccgtggtgaa agcgtttctg
     2641 gccgtgctcg tctccgccgc gtcgttgccg cttctggctc agccgccgcc atcaccgccg
     2701 ccgccgccgt gcacgccggc cgacaacgtc gtttgcggcc aaaacgcgcc cgaggatctc
     2761 gtcgcgctcg gctcgcaatg ggtcgcggcc ggcgcctatg ccggcagtgg cggagtcgtg
     2821 ctgatccgca ccagcgaccg cgcgtcgcac acggcgtatc cggcggccgg cgccgcgaat
     2881 cgccacgata agaagaccta ccccgattgc cccgggccgc ccgggcaggg cggcgcacag
     2941 ttcctgacgc acggcgtcta tgtcgagccc ggcaacgggc ccgtgcacaa gctgttcgtc
     3001 gtcggtcacg gagcacgcga gtcgatcgag gtgctgcaag tcgacacgtc gtcggcgatg
     3061 ccggccgtca cttggctcgg ctgcgtcatt gcgcccgacc cgatcgggtt gaactcggtg
     3121 cgcgggttgc ccgatggcgg cttcatcacg acgaacttct tgccgcgcgg cggcacgcgc
     3181 gaggcgacgc agcaaatgat gggcggcgcg cgcaacggcg agctctggga atggcacacg
     3241 gcgacccgtt ggcagaaggt gcccggcagc gaagcggccg gcgcgaacgg cgtcgagctg
     3301 tcggacgacg gcaagaccct gtacgtcgcc gcctggggca gccaatcgtt cttccgcctg
     3361 gcgcgcggtg cggcgacgcc gacgcgcgat gaaatcccgc tcggctttcg cgtcgacaac
     3421 attcactggg cgcgcgacgg cacgttgtgg gccgtcggtc aggcgacgca gagctggaag
     3481 gccgtcaaag tcgatccaaa aacactcgcg gttcgcgaag tcttgacgca acccgacgcg
     3541 cctgaattcg gcgccggcac tgccgtcgtc gaggtcggca acaagctctg ggcgggctcg
     3601 ttccgcggca accgaatcgt aatactcccg gctccctaac aacggcccgc gtgcacgccg
     3661 cccaattggg cggcgtgcgt ttctctctcg caagcccacg ccgcgttcgg cgcgcgtggc
     3721 acaccagcga cgaggcgctg ctcgatttgc agccgtagcc gtcggacgcg ctagtcgccg
     3781 tacgggctcg cgagcaacgt gcagcgcggc acgcggccgt ccaccacttt cacgccttcg
     3841 ctgcgcagcc tgcgcaattg ctcgcggtag gcgctcgagc ccgacggaaa cgcgatcttg
     3901 ccgccggcgc cgacgacgcg gtgccacggc agatgcaggc cgtccggcgt gtgcttcagt
     3961 gcgtaaccgg cttggcgcgc tcggcccggc aacccggcgg cgcgtgcgac gtcgccatac
     4021 gtcgagacgc tgcccggcgg cagctgcgca acgacgtccc agatcgcctg cagtgccgga
     4081 ttctcgtcga cgccgcgccg cgcgcgcggc ttgcggactg gaagcagcga ccggaccttc
     4141 gtgcgcggca tagtcgatcg gcgtcagcga gccgcttgcg cgaccgccgt cgccgccacg
     4201 tcgccggtgc gcacgcgctt caaaaacggc tcgctggcga cgatcgcttg aatcagagcc
     4261 gcgagcgtcg tgccgtcggc cgcagccgcg cgcacgatcc cgcggacggc gggccggtcg
     4321 tagtattcga gctcgcgccc gagcgcgtac gtcagcaggc gctccgtcaa cgacgtcacg
     4381 aaggcgtcgg agcgcgacaa cagcgcgcgc cgcaggtccg cggctccgcc gagcttcgtg
     4441 ccgtccgtga gctcggccgt cgcgtcgatc ggatggccgt cctcctgctc gcgccaacgg
     4501 ccgacgagat cgaagttctc gagcgccaag ccgagcgggt ccatgatcga atggcacgcc
     4561 gcacaggttg gattcgcacg gtgcatctcg aggcgctcgc gcaccgtgtc gcctttcaac
     4621 ccttcggctt cgctggcttc cttcgcgagg tcggcctcga cgccgggcgg aggcgtcggc
     4681 accgcggcgc ccaagatgtt ctgcacgatc cattggccgc gcacgacggg cgacgtgcgg
     4741 ttcggcgccg acgtcgccgt caagatgctg ccgtggccca acaggccgcg ccgcgggctc
     4801 tcgggcggca gctcgacgcg ccgcatgtag ctgtcgcgaa cgccgtcgat accgtagtgc
     4861 gccgcaagcc gctcgttgag ataggtgtag tcggcgtcga gcagctcgac gacgctgcgc
     4921 cgctcgcgca gcacttcgcc gaacacgagc tcggtctccg cgcgaaaggc cgcgcgcagg
     4981 tccgcgtcga actccgggtc ctgcgacggc acctcgtcga gctcgcgcag cttcaaccat
     5041 tgaccggcga agctctcgac gaagcgctgc gctttgtcgt cggcgagcaa gcgctcgact
     5101 tgcgcggcga gcgtcgcagg ctcgtgcaaa cgtccgtcga cggcgaccgc gagcaactcg
     5161 tcgtccggaa tgctgctcca caggaagaac gacagccggg acgcgagctc gagatcgctg
     5221 atggcgtaga cgtcgcccgt ggcaaggccc gaaggctcgt gctcgaaccg gtagaggaaa
     5281 ttgggatcga cgagcagccg cgacaacgcg tactggatgc cgacctcgaa atcgccgcct
     5341 tccgcgcggc cttgggcgta gaactcgagc agcagcttcg cctcggggct gccggcgccg
     5401 atgggccgcc gataggcccg cgtcgcgagc cgcgtcagta tctgctgcgc gcacggtaac
     5461 gcggccgccg cggtttgcgg ctggcagtcg aagattgcgc ggcggctcgg tgtctcgccg
     5521 gcgcccgttg gctcgtacgg cccgtcaacg acgaggccca tgacggcgcc gccgagctcg
     5581 acctcgccga ggtagagctc cttgacgccg gcacagcgct gctcgtcgac gagcgcggcc
     5641 gtgatgcgct gcgggcct
//
LOCUS       DATLEE010000051         3200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5278041, whole genome shotgun sequence.
ACCESSION   DATLEE010000051 DATLEE010000000
VERSION     DATLEE010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5278041"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="dctP"
                     /locus_tag="VM692_01065"
     CDS             complement(<1..656)
                     /gene="dctP"
                     /locus_tag="VM692_01065"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HUQ50778.1"
                     /translation="MGALLGVLLAGAGLPAHAADIKIATVAPDGSRWIQQMRAGAEEV
                     NAKTAGRVTIKFYPGGVMGNDAQVLRKIRIGQLQGGAFTAGGLAERYGGLNLYGIPLL
                     FRSLEEIDAVRAKLDPELTTGLEQAGFMTFGFIEGGFANVLANEPIHSVDDMRRKKVW
                     VPEGDPISFMVMEALGLSPAVLPVTDVLTGLQTGLIDIAFASPVAALVLQWHTKVKYI
                     T"
     gene            complement(689..1495)
                     /locus_tag="VM692_01070"
     CDS             complement(689..1495)
                     /locus_tag="VM692_01070"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HUQ50779.1"
                     /translation="MAAAVAGCSRLGRVVGNTLTSAILNQSDPALVESGLPAYLLLLD
                     GLISQRSDNIALLSAGAQTFALYGSRFAPRERAAGLTGKARRYGERALCLAHPPACQW
                     QGMEYARFVTELEAVSAKDIGPLYSYAVSWLSQLDATSEDWTAVAELPWVEAVLERAL
                     ALDETYENGALHGYLGIINALRPPALGGKPDVAREHFERAIELSGGRDLSIKVEYARR
                     YARLVFDQELHDRLLTEVLNAPVEAPLYTLFNVLAKQEAQALLATSKEYF"
     gene            complement(1560..1904)
                     /gene="rplS"
                     /locus_tag="VM692_01075"
     CDS             complement(1560..1904)
                     /gene="rplS"
                     /locus_tag="VM692_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HUQ50780.1"
                     /translation="MSRLIDQIEQQQLKQDVPDFAPGDTVVVQVRVTEGDRERLQAYE
                     GIVIAKRNRGLNSSFTVRKVSHGEGVERVFQTHSPAVAEIKVKRRGDVRRAKLYYLRD
                     LSGKAARIKEKV"
     gene            complement(1968..2726)
                     /gene="trmD"
                     /locus_tag="VM692_01080"
     CDS             complement(1968..2726)
                     /gene="trmD"
                     /locus_tag="VM692_01080"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HUQ50781.1"
                     /translation="MYRFGVVSLFPELVAPVMEFGVVGRAAERGLIALEQSSPREFAT
                     DRHRTVDDRPYGGGPGMVLKYEPLRDAVRTVRRRLPSGCREFVLAAQGRPFDQALARE
                     LAVAPGLLLVCGRYEGIDERFTDLEVRDELSLGDYVLSGGELAAAVVIDAVTRLLPGV
                     LGDESSSVEDSFTAGLLDCPQYTRPEVVDSAQVPEVLLGGDHAAIRRWRLKQALGRTW
                     LRRPELVASRVLTGEEQELLEQFRAEWRELNSRS"
     gene            complement(2736..>3200)
                     /gene="rimM"
                     /locus_tag="VM692_01085"
     CDS             complement(2736..>3200)
                     /gene="rimM"
                     /locus_tag="VM692_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785744.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HUQ50782.1"
                     /translation="VFGVKGWLKVQSYTEPRDNVVDFAVWTLRMNGADRPFEVEDGQG
                     HAGKVVAKLRGIDDPERAREWLGAEIVVEREQFPRAQAGEFYWTDLEGLEVRTTAGVM
                     LGRVAYLLATGANDVLVLGGTPERLIPFLLGTVVQQVDFEAGLIVVDWSTDD"
BASE COUNT          586 a         1112 c          943 g          559 t
ORIGIN      
        1 gtgatgtatt tgaccttggt gtgccactgc agcaccagcg cagccacggg cgacgcgaag
       61 gcgatgtcga tgaggcccgt ctgcaagccg gtcagcacgt cggttaccgg tagcacggcc
      121 ggcgacaagc ccaaagcttc catgaccatg aagctgatcg gatcgccctc gggcacccag
      181 accttcttgc ggcgcatgtc gtcgacgctg tgaatcggct cgttggcgag cacgtttgcg
      241 aaaccgccct cgatgaaccc gaaggtcatg aagccggcct gctcgagacc cgtcgtcagc
      301 tcgggatcga gcttggcgcg cactgcgtcg atctcctcga gcgagcgaaa caacaatggg
      361 atgccgtaga gattcagtcc gccgtagcgc tcagcaagcc cgcctgccgt gaaggcgccg
      421 ccttgcagct gaccgatgcg gatcttgcgc agcacttgcg cgtcgttgcc catgacgccg
      481 ccgggataaa acttgatcgt tacgcgaccc gcagtcttcg cattgacctc ttcggcgccc
      541 gcgcgcattt gctgaatcca gcgcgagccg tcgggcgcga ccgttgcaat tttgatgtcg
      601 gccgcgtgtg cgggcaggcc tgcgccggcc aacaacacgc cgagcaaggc tcccatcagt
      661 gaacgagctc gtcctgcgca ctgccgcatc agaagtactc cttcgaggtc gcgagcaagg
      721 cctgagcttc ctgctttgcc aacacgttga acagcgtata cagcggcgct tcgaccggag
      781 cgttcagcac ttccgtcaac aggcggtcgt gcagctcctg gtcgaacacg agacgcgcgt
      841 aacgccgcgc atattctacc ttgatcgaga ggtcacggcc gcctgacagc tcgatcgcgc
      901 gctcgaaatg ctcgcgtgcg acatcgggct tgccgccgag cgcgggcggg cgcagcgcat
      961 tgatgattcc gagatagccg tgcaacgcgc cgttctcgta cgtctcgtcg agcgcgagtg
     1021 cccgctcgag cacggcttcg acccagggca gttcggcgac cgccgtccaa tcttcactgg
     1081 tcgcatcgag ttggctcagc cagctgacgg cgtacgaata cagcggaccg atgtctttcg
     1141 cgctgacggc ctcgagctcg gtgacgaagc gcgcgtactc catgccctgc cattggcaag
     1201 ccggtgggtg cgcgaggcaa agcgcgcgct caccgtaacg acgtgctttg cccgtcagcc
     1261 cagcggcgcg ctctctcggc gcgaaccgcg agccgtagag cgcgaatgtc tgcgctccgg
     1321 ccgagagcaa tgcgatgttg tccgatcgtt gcgatatcaa cccgtcgagc aacagcagat
     1381 acgcgggcaa gccgctctcg acgagcgccg ggtcgctttg attgaggatg gcgctggtta
     1441 gcgtgttgcc cacaacacgg cccaagcgcg agcagccggc caccgcggcg gccacgacgc
     1501 aagccacaac gagcctgcga agcatcgggc cgacgcagac cttcgctgcg ccgagcgtgc
     1561 tagaccttct ctttgatgcg ggcggccttg ccgctgagat cgcgcaggta atagagcttg
     1621 gcccggcgga catcgccacg tcgtttcacc ttgatttccg ctacagcggg actgtgcgtc
     1681 tggaacacgc gctccacgcc ctcgccatga gaaacctttc tgaccgtaaa cgaagagttc
     1741 agcccccggt tacgcttcgc gatcacgatt ccttcgtatg cctggagccg ctcgcgatcg
     1801 ccttctgtca cgcgcacttg gacgaccacg gtgtcccccg gcgcaaagtc gggaacgtcc
     1861 tgtttcagtt gttgctgttc gatctggtcg attaacctgg acataacatt atcctctgcc
     1921 attctcgttc ggtggccgtt gcgcggaatc acactattct acgcaatcta gctgcgcgaa
     1981 ttcagctcgc gccattccgc cctaaattgc tccaacaatt cctgctcctc gcccgtcagc
     2041 acccggctcg ccacgagctc cgggcgccgc agccaagtgc gtccgagcgc ttgcttcagc
     2101 cgccagcgcc tgatcgccgc atggtcgccg ccgagtagca cctccggcac ttgcgcggag
     2161 tcgacgacct ccggccgcgt gtactgcggg cagtcgagca gccccgccgt aaacgaatcc
     2221 tcgacgctcg agctttcgtc gccgagaact ccgggcaaga gacgcgtgac agcatcgatc
     2281 acgaccgccg ccgccagctc gccgcccgag agcacatagt cgcccagcga caactcgtcg
     2341 cgaacctcga gatccgtaaa ccgctcgtcg atcccctcgt aacgtccgca gacgagcagc
     2401 aaacccggcg cgaccgcaag ctcgcgcgcc aacgcctgat cgaacggcct gccctgtgct
     2461 gcgagaacga actcgcggca gccgctcggc aaacgccgac gtaccgtgcg cacggcgtcg
     2521 cgcagcggct cgtacttgag caccatgccg ggtccgccgc cgtatggacg atcgtcgacc
     2581 gtgcgatgac gatccgtcgc gaactccctc gggctcgact gctcgagcgc aatcaagccg
     2641 cgctcggccg cgcgcccgac gacgccgaat tccatgaccg gcgccacgag ctccggaaac
     2701 agcgagacca caccgaaccg atacatctgc agtgctcaat cgtcggtcga ccagtcgact
     2761 acgatcaggc ccgcctcgaa atcgacctgc tggacgaccg ttccgaggag aaacggtatc
     2821 aagcgttctg gcgtaccgcc gagcacgagc acgtcgttcg cccccgtcgc cagcagatac
     2881 gccacgcggc cgagcatgac accggctgtc gtgcgaacct cgagcccttc gagatccgtc
     2941 caatagaact cgccggcttg cgccctcggg aactgctccc gctcgacgac gatttcggcg
     3001 ccgagccatt cgcgagcgcg ttccgggtca tcgatcccgc gcagcttcgc gaccactttg
     3061 cccgcgtggc cctgaccatc ctctacctcg aacgggcggt cggcaccgtt catgcgcaac
     3121 gtccaaacgg cgaaatcaac gacgttgtcg cggggttccg tgtaggactg cactttgagc
     3181 caccctttca cgccgaacac
//
LOCUS       DATLEE010000052         4367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3787442, whole genome shotgun sequence.
ACCESSION   DATLEE010000052 DATLEE010000000
VERSION     DATLEE010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3787442"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /gene="truB"
                     /locus_tag="VM692_01090"
     CDS             complement(<1..802)
                     /gene="truB"
                     /locus_tag="VM692_01090"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HUQ50783.1"
                     /translation="MQATRSPSRDVFGLLLLDKPAGLSSNRALQRAKRLFGAAKAGHT
                     GSLDPLATGMLPIFFGAATRLASHMLEASKTYRVTARLGIATATGDAEGATTEDRSQA
                     PAPDAATLSAIAGRFVGELEQVPPMYSALKRDGVPLYRLARAGVEVERAPRRIVIDEL
                     VIERYRWPELDFRVRCSKGTYVRTLVEDLAKAAGTVGHVAALRRVAVDPFDGHALQTF
                     EALEAAAMGGSGALDALLLPADAALPGWPSASIEAAEVGRLTQGQAVQA"
     gene            complement(807..1172)
                     /gene="rbfA"
                     /locus_tag="VM692_01095"
     CDS             complement(807..1172)
                     /gene="rbfA"
                     /locus_tag="VM692_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HUQ50784.1"
                     /translation="MPREFARKHRVAAEIQRLLNELLLAEVKDPRLTNARVSDVEVTG
                     DLSLATIHFNTLGLDDDPVPIKEGFEQARGFFRSRVARALQLRRVPELRFRHDTSARR
                     AVEVGKLIDDARGTQRGTD"
     gene            complement(1172..3715)
                     /gene="infB"
                     /locus_tag="VM692_01100"
     CDS             complement(1172..3715)
                     /gene="infB"
                     /locus_tag="VM692_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HUQ50785.1"
                     /translation="MAEVTVADFAKVLKVPVDRLIEQLDEAGIKVGGPDDVISEDAKL
                     ELLTHLKKNHGRQDAADSAPRRITLNRKSSSELKLASGQGRARTINVEVRQKRTYIKR
                     DVLEEQARKQQEELDAVRRVEEETRNAEERSVREEREKREAAERERVAAEQREAEERK
                     RLEDEQRRAAAADQAAKQRAEEERRKAATAEKEAQQRAEAERARQKAKKERMVEEPAD
                     GDHTLHVADGMAGRRKKKKQRRRSVAVNVDPQHGFEKPTAPVVRDVEIGETITVSDLA
                     QRMAIKANEVIKALMKMGMMVTINQVLDQDTATLVVEEMGHSAKPLKETAIEDELIQS
                     VEEASSSSAAVTRAPVVTIMGHVDHGKTSLLDYIRRTKVAAGEAGGITQHIGAYNVET
                     PKGRITFLDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTIEAVQHAKAAKVPI
                     VVAVNKIDKPGGDLDRIRRELSNYELISEEWGGETLFVSVSALTGQGVDKLLDSLLLQ
                     AELLDLKAVDTGPAAGVVLEASLERGRGAVATVLIDKGKLQVGDILLAGQEYGRIRAM
                     FNETGQSIPFAGPSTPVVVLGLSGTPMSGDDVQVVEDERQARDVAQFRHSRERETKLA
                     HQQAAKLENIFSQMQAGAVPSVQLLIKADVHGSAEALRDALEKITSEDVKITVVSSGV
                     GGITESDVQLAATSKAIIIGFNVRADAAARGAIKESGVDIRYYSIIYEAIDDIKAAAS
                     GLLAPEIREQIVGLAEVREVFRSPKFGNVAGCMVVDGYVRRSNPIRVLRNNVVIYEGQ
                     LESLRRFKDDVNEVRAGTECGIGVRNYNDVKAGDQIECYERTEIVRKVS"
     gene            complement(3727..>4367)
                     /locus_tag="VM692_01105"
     CDS             complement(3727..>4367)
                     /locus_tag="VM692_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HUQ50786.1"
                     /translation="AMSPAEVLSIVVDEDTHTMDLAVEEEKLSQAIGRGGQNIRLASE
                     LSGWQLNVMTESAAEEKSDKEARSLVENFMKELDVDEDVANILVQEGFSSLEEIAYVP
                     ASELNGIEEFTPEIVNELRSRARDVLITKALVSEEVIDQAEPAEDLLSLEGMDRTLAL
                     ELASRGVVTREDLAEQAVDDLSEIAGLDADRAAKLIMAARAHWFEQEQQAKG"
BASE COUNT          620 a         1531 c         1367 g          849 t
ORIGIN      
        1 gggcctgaac ggcctggccc tgtgtcagcc gcccgacctc cgccgcttcg atgctcgccg
       61 acggccagcc cggcagcgcc gcgtcggccg gcagcaggag cgcgtccaag gccccggacc
      121 cgcccatagc cgcggcctcg agcgcctcga acgtctgcag cgcgtgcccg tcgaacgggt
      181 ctacggccac gcgccgaagc gccgcgacgt gccctaccgt tcccgcggcc ttcgcgagat
      241 cctcgacgag cgtcctgacg taggtgccct tcgaacaacg gacgcgaaag tcgagctccg
      301 gccagcggta ccgctcaatt acgagctcgt cgatgacgat gcggcgcggt gcacgctcca
      361 cttcgactcc ggcgcgcgcg aggcggtaca gcggcacgcc atcgcgcttc aacgcggagt
      421 acatcggcgg gacttgctcg agctcgccga cgaaccggcc cgcgatggcc gacagcgtcg
      481 ccgcgtccgg cgccggtgcc tgcgaccggt cctcggtcgt cgcgccttcg gcgtcgccgg
      541 tggcggtcgc gatgccgagc ctcgccgtga cgcgataggt cttgctggcc tcgagcatgt
      601 ggctcgcgag ccgcgtcgca gcgccgaaga agatcggcaa cattccagtc gccagcggat
      661 cgaggctgcc cgtatggccg gctttcgcgg ccccgaacaa gcgcttggcg cgctgcagcg
      721 cgcggttcga ggacaggccg gcgggcttgt cgagcagcag gagcccgaag acgtcgcggc
      781 tcggtgagcg cgtggcctgc aaagggtcag tcggtgccgc gctgcgtgcc ccgcgcgtcg
      841 tcgatcaatt tcccgacctc gacggcacgt cgagcgctcg tgtcgtgacg gaagcgcagc
      901 tccggcacac gacgcagctg cagcgcccgc gcgacgcgcg agcggaagaa gccgcgcgct
      961 tgctcgaacc cttccttgat cggaacaggg tcgtcgtcga gccccagagt attgaaatga
     1021 atcgtcgcga ggctcagatc gccggtgacc tcgacgtcgc tgacgcgcgc gttggtcagc
     1081 cgtggatcct tgacctccgc gagcaacagc tcgttgagca gccgctgaat ttcggcagcg
     1141 acacgatgct tccttgcgaa ttcacgtggc atcagctcac cttgcgtact atctccgtac
     1201 gttcatagca ctcgatctga tcgccggcct tgacgtcatt gtaattccga acgccgatac
     1261 cgcactcggt gccggcccgg acctcgttga cgtcatcctt gaaccggcgc agcgactcga
     1321 gctgcccttc gtagatgacg acgttgttgc gcagcacgcg gatcgggtta ctgcggcgca
     1381 cgtaaccgtc aacgaccatg cagcccgcaa cgttgccgaa cttcggcgag cggaacacct
     1441 cgcgcacctc ggccaggcca acgatctgct cgcggatctc cggcgccaac aagccgctcg
     1501 ccgcggcttt gatgtcgtcg atcgcctcgt aaatgatgct gtagtagcgg atgtcgacgc
     1561 cgctttcctt gatggcgccg cgcgcagccg catcggcacg cacgttgaag ccgatgatga
     1621 tcgccttcga cgtagccgcg agctgcacgt cggactccgt gatgccgccg actccggagc
     1681 tgacgacagt gattttaacg tcctccgacg taatcttctc gagcgcgtcg cgcagcgctt
     1741 cggcgctgcc gtggacgtcg gccttgatca gcagttggac gctcggcacc gcgccggcct
     1801 gcatctggct gaagatgttc tcgagcttgg cggcttgctg atgggcgagc ttcgtttcgc
     1861 gctcgcggct atgacggaac tgcgccacgt cgcgcgcctg tcgctcgtcc tcgacgactt
     1921 gcacgtcgtc gccggacatc ggcgtcccgg acagacccag cacgacgaca ggcgtcgacg
     1981 ggcccgcgaa cggaatcgac tgacccgtct cgttgaacat tgcccggatg cgcccgtatt
     2041 cctgcccagc cagcagtatg tcgccgacct gcagcttgcc cttatcgatg agcacggtgg
     2101 cgactgcgcc gcggccgcgc tcgaggctcg cctcgaggac gacgccggcg gccgggccgg
     2161 tgtcgactgc cttcaggtcg aggagctcgg cttgcaacaa tagcgagtcg agcagcttat
     2221 cgacgccctg gcccgtcagt gccgagacgc tgacgaacaa ggtctcgcca ccccactctt
     2281 cggagatcag ctcgtagttc gaaagctcgc ggcggatgcg gtcgaggtcg ccgccgggct
     2341 tgtcgatctt gttgactgca acgacgatcg gcaccttcgc cgccttcgcg tgctggaccg
     2401 cctctatcgt ctgcggcatg acgccgtcgt cggccgcgac caccagcacg acgatgtccg
     2461 ttactttggc gccgcgcgcc cgcattgccg tgaaagccgc gtggcccggc gtatcgagga
     2521 acgtgatgcg gcctttcggc gtctcgacgt tgtaggcacc gatatgctgc gtgatgccgc
     2581 cggcctcgcc cgcggcgact ttcgtgcgcc ggatgtaatc gagcaatgac gtcttgccgt
     2641 gatcgacgtg gcccatgatc gtgacgaccg gtgcgcgagt caccgctgcc gacgagctcg
     2701 aagcttcctc gaccgactga atcagctcgt cctcgatcgc cgtttccttg agcggcttcg
     2761 cgctgtggcc catctcttcg acgacgagcg tcgcggtgtc ctggtcgaga acctgattga
     2821 tcgtgaccat catgcccatc ttcatgaggg ccttgatgac ctcgttggct ttgatggcca
     2881 tgcgctgcgc caggtcgctg accgtgatcg tctcaccaat ctcgacgtcg cgaacgaccg
     2941 gcgcagtggg cttctcgaac ccgtgctgcg ggtcgacgtt gacggcgacc gaacgacggc
     3001 gctgcttctt tttcttgcgc cggccggcca tgccgtcggc gacgtgcaac gtgtggtcgc
     3061 cgtctgcggg ctcttcgacc attcgctctt tcttggcctt ctgccgcgcg cgctcggctt
     3121 cggcgcgctg ctgcgcttcc ttctcggccg ttgctgcttt gcgacgttcc tcttcggcgc
     3181 gctgtttagc cgcctgatcg gcggcggccg cgcggcgctg ctcgtcctcg aggcgcttgc
     3241 gctcttcggc ctcgcgctgc tcggccgcta ccctctcgcg ctccgccgct tcgcgcttct
     3301 cgcgctcttc gcgcaccgaa cgctcctcgg cgttgcgcgt ttcctcttcg acgcggcgca
     3361 ccgcgtcgag ctcttcctgc tgcttgcgtg cctgctcctc gagcacgtcg cgcttgatgt
     3421 aggtgcgctt ctggcggacc tcgacgttga tcgtgcgcgc gcgaccttgg ccgctcgcga
     3481 gcttgagctc gctgctcgat ttgcgattca gcgtgatgcg gcgcggcgcc gagtccgccg
     3541 catcttggcg gccgtgattt ttcttgaggt gcgtcaacag ctcgagcttc gcgtcctcgc
     3601 tgatgacgtc gtcgggaccg cccaccttga tgccggcctc gtcgagctgc tcgatgagcc
     3661 ggtcgacagg aaccttcagc actttcgcga aatccgcgac cgtaacttcc gccatgcgtg
     3721 tctacctcag ccctttgcct gctgctcttg ctcgaaccag tgagcacgcg ccgccatgat
     3781 caattttgcc gccctgtccg cgtcgagccc ggcgatctcg ctgagatcgt cgacggcctg
     3841 ctcggccaga tcttcgcgcg tcacgacgcc gcggctcgca agctccagcg cgagcgtccg
     3901 gtccatgccc tcgaggctca acagatcctc ggcgggctcc gcctggtcga tgacttcctc
     3961 gctgacgagc gccttcgtga tcagcacgtc gcgggcgcgc gagcgcagct cgttgacgat
     4021 ctccggcgtg aactcttcga tgccgttgag ctcgctggcc gggacgtaag cgatctcctc
     4081 gagcgacgag aagccttctt gcacgaggat gttcgcgacg tcctcgtcga cgtcgagctc
     4141 cttcatgaag ttctcgacga gcgagcgcgc ttccttgtcg ctcttctcct cggccgccga
     4201 ctcggtcatg acattcaact gccaccccga tagctcgctg gcgagccgga tattctggcc
     4261 gccgcgcccg atggcctgcg acagcttctc ctcctcgacc gcgagatcca tcgtgtgcgt
     4321 gtcctcgtcg acgacgatcg acagcacctc ggccggcgac atcgcgt
//
LOCUS       DATLEE010000053         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4531631, whole genome shotgun sequence.
ACCESSION   DATLEE010000053 DATLEE010000000
VERSION     DATLEE010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4531631"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..248)
                     /locus_tag="VM692_01110"
     tRNA            complement(172..248)
                     /locus_tag="VM692_01110"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(212..214),aa:Pro,seq:ggg)
     gene            complement(645..944)
                     /gene="ihfA"
                     /locus_tag="VM692_01115"
     CDS             complement(645..944)
                     /gene="ihfA"
                     /locus_tag="VM692_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HUQ50787.1"
                     /translation="MALTKANMAESLFNELGLNKREARELVDSFFEDLRAALAVGEQV
                     KLSGFGNFDLRDKNKRPGRNPKTGEEIPITARRVVTFRPGQKLKARVEAYAGTRE"
     gene            complement(963..>2590)
                     /gene="pheT"
                     /locus_tag="VM692_01120"
     CDS             complement(963..>2590)
                     /gene="pheT"
                     /locus_tag="VM692_01120"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HUQ50788.1"
                     /translation="TNYVMLELGQPLHAYDLAKLDGKIEARLARANEKLTLLDGRAIE
                     LADDMLVIADRRGPVGLAGVMGGESTAVSETTDSVFLESAFFAPAAIIGRARRIGLHT
                     DASLRFERGVDPTGQVRAIERATELLLAICGGQAGPVSNVERSADLPQRSAVALRRDR
                     LRAVLGVAVPDSEVAAIFARLELQATARPDGWLVTPPAFRFDVAIEEDLIEEVGRMVG
                     YDAIPATPAEPVERLGRASEHAVEADRVADLLAARGYAEAITYSFIDANLEELVNPGA
                     ERVEVANPIASDMAVLRRSLWPGLINAARLNVTHQRQRLRLFELGPQFSAAGRGVTQT
                     AVAAGLALGTRAPEQWDGGAVAEVDYFDVKGDVEAVLRLTGASAEFRFEPARHPALSP
                     GRTARILRGDRPVGWIGALHPELAKRVDKKRTAVVFALQLEGLAPALVPAYRRYSKYP
                     SIRRDLAIVVDNSVSAEALTKSARAAAGDILQHVLVFDVYRGKGVDSRQKSVGLGLIL
                     QESSRTLTDEDADQKMRSVMQRLEREFGATIRT"
BASE COUNT          437 a          855 c          830 g          468 t
ORIGIN      
        1 gacgcgaaca ccggcctcgc ggcgtctgcg gcaaacgggt tcgtttcgag cttgtactgt
       61 tcgagaaaca aaggacgccg agtccggtat tcgttgttat tgagcggttg ttacgggaat
      121 cgctgcccca cgatacccgg agtgctccaa ccggactaga ggaaaacgaa ttggtcgggg
      181 cgacaggatt tgaacctgcg accccttgca ccccatgcaa gtgcgctacc aggctgcgcc
      241 acgccccgac cgaggtagca agtttaacga taaagcggcc ggacccgata cccgggggcc
      301 cgaaccgcga gcggcgcagc agcttgagaa cgtcctcgag ctcggcgcga atctggcgaa
      361 tgatctgttg gctttggctc gtatcgctgc gagcttcgtc gcccatgagc cgctgccggg
      421 cgccgccgat cgtgaacccc tcttcgtaaa ggaggctgcg gatctggcgg atgatcaaga
      481 cgtcctggcg ctgataatag cggcggttac cgcggcgctt gacgggcttc agctgcggga
      541 actcttgctc ccaatagcgc agcacgtgcg gcttgacggc gcacagctcg ctgacctcgc
      601 caatcgtgaa gtagcgcttg cccggaattg gcgggaggtc gtcgttattc cctggttcca
      661 gcataggctt cgactcgcgc ttttagtttt tgaccggggc gaaacgtcac gacgcggcga
      721 gcggtgatcg gaatctcctc gcccgtcttc ggatttcggc caggtcgctt gttcttgtca
      781 cgcaaatcga agtttccgaa tccggaaagc ttgacttgtt ccccgacggc cagtgccgct
      841 ctcaaatctt cgaaaaagga atctacgagc tcgcgtgctt cacgcttgtt caagccgagc
      901 tcgttgaaca acgactcggc catgttcgct ttcgttaatg ccatctcggc gtccgattct
      961 tgttaggttc ttatcgtcgc cccgaactca cgctcgagtc tctgcatgac ggagcgcatc
     1021 ttttgatcgg cgtcctcgtc agtaagcgtg cgtgatgact cctgcaaaat caagcctaaa
     1081 cccacgcttt tttgccttga atctaccccc ttgccacggt atacgtcgaa cacgagaacg
     1141 tgctgcaaga tgtcgccggc ggctgcgcga gcagattttg tcaaagcctc ggcgctgacg
     1201 ctgttgtcga cgacgatcgc gagatcgcgc cggatcgacg gatacttcga gtacctgcgg
     1261 tacgccggca cgagcgcggg cgcgagtccc tcgagctgca gcgcgaacac gacggccgtg
     1321 cgtttcttgt ccacgcgctt cgcgagctcc gggtgcagcg cgccgatcca accgacgggc
     1381 cgatcgccgc gcaggatgcg tgccgtacgc ccggggctca aggctgggtg ccgggccggt
     1441 tcgaagcgaa actcggcact agcgcccgtc aggcgcaaca cggcctcgac gtcgcccttg
     1501 acgtcgaagt agtcgacttc ggccacggcg ccgccgtccc actgctccgg cgcgcgcgtg
     1561 ccgagcgcca agccggcggc gacggctgtc tgcgtaacac cccgccctgc ggccgagaac
     1621 tgcgggccga gctcgaatag gcgcagtcgc tgccgctgat gcgtgacatt gagccgcgcc
     1681 gcattgatga gccccggcca gagcgagcgg cgcagcacgg ccatgtcgct ggcgatcggg
     1741 ttcgcgactt cgacgcgctc ggcgcccgga ttgacgagct cttccaaatt cgcatcgatg
     1801 aagctgtagg tgatggcctc ggcgtagccg cgcgcggcaa gcaagtcggc gacgcgatcc
     1861 gcttccacag cgtgctcgct cgcgcggccg agccgctcga cgggctcggc gggcgtcgcc
     1921 gggatcgcgt cgtagcccac catgcggccg acctcctcga tcaggtcttc ctcgatcgcg
     1981 acgtcgaagc gaaatgccgg cggcgtcacg agccaaccgt cggggcgcgc ggtcgcttgc
     2041 agctcgagac gggcgaagat cgccgccacc tcggagtccg gcactgctac gccgagcacc
     2101 gcgcgcaatc ggtcgcggcg caacgcgacg gcggaccgct gcggtaaatc ggcgctgcgc
     2161 tcgacgttcg agaccgggcc cgcttgcccg ccgcaaatcg cgagcagcaa ctccgtcgcg
     2221 cgctcgatcg cgcgcacttg ccccgtcggg tcgacgccgc gctcgaagcg cagcgacgcg
     2281 tctgtgtgca gaccgatgcg ccgcgcacgg ccgatgatgg cggccggcgc gaagaacgcg
     2341 ctctcgagga acaccgagtc cgtcgtctcg ctgacggccg tcgactcgcc gcccatgacg
     2401 ccggccaagc cgacgggccc gcgcctgtcg gcgatcacga gcatgtcgtc ggcgagctcg
     2461 atcgcgcgcc cgtcgagcag agtgagtttt tcgttcgcgc gcgcgagccg tgcctcgatc
     2521 ttgccgtcga gcttggcgag atcgtaggcg tgcagcggct gcccgagctc gagcatcacg
     2581 tagttcgtca
//
LOCUS       DATLEE010000054         2326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3789667, whole genome shotgun sequence.
ACCESSION   DATLEE010000054 DATLEE010000000
VERSION     DATLEE010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3789667"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VM692_01125"
     CDS             complement(<1..1449)
                     /locus_tag="VM692_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HUQ50789.1"
                     /translation="MTANTAVSLDEQIKRLMAHLEHEQPQLLKVVQTFRELDKVAYQL
                     GLLDPTESYATQVSWWPLVAVLGTFSSGKSTFINNVLGRRLQATGNQAVDDKFTVICY
                     SPDSTPHVLPGLALDSDPRFPLYRISDEIDAELKGESQRLDTYLQLKTADSEALRGRI
                     FIDSPGFDADEQRTATLRITNQIIDISDLVLVFFDARHPEPGAMRDTLEHLVSGTLER
                     ADRNKCLYILNQIDNAAREDNPEEVFAAWQRALAQKGLTAGRFYQIYDPASAIPIENE
                     HVRRRFEAKREHDMAEILGRIRQLEVERSYRVTARLEQTAEAIREAIVPRVVAARRAW
                     KRRVWWLDAAAFGGLAALALVLVTINGVWGDIGALADRLEANAVAGWLVVLAVVVAAW
                     LLHRLMRNIAARSVLRQIESDASLGAHATRVARAFRRNARSWRPFFAAQPVGWSRGVR
                     RRLDQVLSSAHAAVQELNDRYTDPSGRSQMSGQ"
     gene            complement(1582..>2326)
                     /locus_tag="VM692_01130"
     CDS             complement(1582..>2326)
                     /locus_tag="VM692_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUQ50790.1"
                     /translation="GLFPLIRDLFASLPDSKVRGYPPGRFSFNVKGGRCETCQGDGVI
                     KVEMHFLPDVYVPCDTCGGKRYNRETLDIRYKGKNISEVLKLTVEDALALFRNVPMVA
                     HKLETLMAVGLGYIELGQNATTLSGGEAQRVKLAKELSKRSTGSTLYVLDEPTTGLHF
                     HDIAQLLEVLYKLRDQGNTIVIIEHNLDVIKTADWIVDLGPEGGAGGGEIIAVGTPEQ
                     IAAEPHSYTGQALRAALPQNAPGRRAAAT"
BASE COUNT          397 a          745 c          750 g          434 t
ORIGIN      
        1 ctgtccgctc atctggctgc ggccagacgg gtccgtgtag cgatcgttga gctcttggac
       61 tgctgcgtgc gcgctgctca aaacctgatc gaggcgccgc cgcacgccgc ggctccaacc
      121 aacgggctgc gcagcaaaga acggccgcca tgaccgtgca ttgcgccgga acgcgcgcgc
      181 gacgcgagtg gcgtgggcgc cgagcgacgc atcgctctcg atctgtcgca acacgctacg
      241 cgccgcgatg ttccgcatga gccggtgcag caaccatgcg gcaacgacaa ccgctagaac
      301 tactagccag cccgcgaccg cattcgcctc caaccgatcg gctagggctc caatgtcgcc
      361 ccagacgccg ttgatcgtga caagcactag agccaatgcg gcaagaccgc cgaaagcggc
      421 cgcgtcgagc caccacaccc ggcgcttcca agctcgtcga gcagcaacca cgcgcggcac
      481 gatcgcctcg cggatagcct cagcggtttg ctcgagccgt gcggtgacac gataagaccg
      541 ttcgacttcg agctgccgta tgcgcccaag aatctcggcc atgtcgtgtt cgcgcttcgc
      601 ctcgaagcgc cggcgcacat gctcgttctc gatcggtatg gccgacgccg gatcgtaaat
      661 ctgatagaag cgccctgctg tcagaccttt ttgcgcgagc gcacgctgcc aggccgcgaa
      721 cacttcttcc ggattgtcct cccgagcggc gttgtcaatc tggttcaaga tgtaaaggca
      781 cttgttgcgg tccgcgcgct ccaatgtgcc gctgacgaga tgctcgagcg tatcccgcat
      841 ggcaccgggc tccggatggc gcgcatcgaa gaacacgagc acgagatcgg agatgtcgat
      901 gatctgattc gtgatccgca acgtcgcagt gcgctgctcg tcggcatcga atccggggga
      961 gtcgatgaaa atgcggccgc gtagtgcctc gctgtccgca gtctttagtt gcaggtaggt
     1021 atcgagccgc tggctctctc ccttcagctc cgcgtcgatc tcgtcgctga tgcggtagag
     1081 cggaaagcgg ggatcgctgt cgagggcgag gcccggcaag acgtgaggcg tgctgtctgg
     1141 gctgtagcag atcacggtga atttgtcgtc gacggcttgg ttgccggtcg cctgcagccg
     1201 tcgcccaagc acgttgttaa tgaacgtcga cttgccggag gagaacgtgc cgagcacggc
     1261 gacgagcggc caccacgaca cctgcgtcgc atacgattcc gtcggatcga gcaagccgag
     1321 ctggtacgcg accttgtcca gctcgcggaa cgtttggacg accttcagga gctgcggctg
     1381 ctcatgctcg aggtgcgcca tgaggcgctt gatctgctcg tcgaggctta ccgccgtatt
     1441 ggccgtcatc cgtagatctc cgtggcgacg cacgcgaggt accccgcctc cagatgcgga
     1501 cggggacgca cgagtgaacc ggtgactgcc ttattgcaat gttcgtgccg gccgcgggga
     1561 gcttgcggtg gttcggctga cctacgtcgc ggccgcgcgc ctgcccggcg cgttctgagg
     1621 tagcgccgct cgcagcgcct ggccggtgta ggaatgcggc tcggccgcga tctgctccgg
     1681 cgtgccgacg gcgatgatct cgccgccgcc ggcgccgcct tcgggcccca agtcgacgat
     1741 ccaatccgcc gttttgatga cgtcgagatt gtgctcgatg atcacgatcg tgttgccctg
     1801 gtcgcggagc ttgtagagca cctcgagcag ctgcgcgatg tcgtggaagt gcagccccgt
     1861 cgtcggctcg tcgagcacgt acagcgtcga gcccgtcgag cgcttcgaca gctccttcgc
     1921 gagcttgacg cgctgcgctt cgccgccgct caaagtcgtc gcgttctgcc cgagctcgat
     1981 gtagcccaac ccgaccgcca tcagcgtctc gagcttgtgc gcgaccatcg gcacgttgcg
     2041 gaacagcgcg agcgcgtctt cgacggtcag cttcaacact tcgctgatgt tcttgccttt
     2101 gtagcggatg tcgagcgtct cgcggttgta gcgcttgccg ccgcaggtgt cgcacggcac
     2161 gtagacgtcg ggcaggaagt gcatctcaac cttgatgacg ccgtcgcctt ggcacgtctc
     2221 gcagcgaccg cctttgacgt tgaagctgaa gcgaccgggc gggtagccgc gaactttgga
     2281 gtcgggcagg gaagcgaaaa gatcgcgaat caagggaaag aggccg
//
LOCUS       DATLEE010000055         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3791923, whole genome shotgun sequence.
ACCESSION   DATLEE010000055 DATLEE010000000
VERSION     DATLEE010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3791923"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /gene="metG"
                     /locus_tag="VM692_01135"
     CDS             complement(<1..1574)
                     /gene="metG"
                     /locus_tag="VM692_01135"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HUQ50791.1"
                     /translation="MSVKRTILVTSALPYANSSLHLGHVVEYVQTDVWVRFQRLRGHR
                     CLYVCASDAHGTPTMLRAEQEGTTPEALVAGVTEEHKRDFATYRISVDNYLTTHSPEN
                     EQLTTELYRRLAAGGFITRKTIKQAYDVERQMFLPDRYVKGTCPNCGTPDQYGDSCES
                     CGATYSPLELKDAVSTVSGTKPSVRESEHLFLKLSAFTAELEKWVPEHVDAVLARKLD
                     EWFKAGLKDWDISRDAPYFGFRIPGERDKFFYVWFDAPIGYMASFLSLCKREGIDFDE
                     FWGADSRHELYHFIGKDIPYFHALFWPAMLSGAGYRKPSGLFVHGHVTVDGQKISKRR
                     GNSVTAADFAKAIDPDYLRYYYASKLGPTIDDLDLNFADFIAKVNSDLVGKLVNIASR
                     CAGFIEKLNGGRLAAELPEPALYREFAAAADDLAADFEGREYSRAIRKIMALADRANQ
                     YIDEHKPWVLAKQPGAEAEVVAVCTQGVNLFRALIVYLKPVVPALAARAEKLLGGGEL
                     RWQDAAQPLLGTRIAK"
     gene            complement(1662..>2198)
                     /locus_tag="VM692_01140"
     CDS             complement(1662..>2198)
                     /locus_tag="VM692_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HUQ50792.1"
                     /translation="SQREVGVHSPSFAHALHSALREDPDAILVGELRDLDTMGIAMTA
                     AEMGVLVMGTLHTNGAAATIDRVVNIFPADKQDHVRNMLSTSLRGIVSQQLLKKKDGT
                     GRVAALEILINTSAAANLIRQGKLEQLENVMQSSGREGMCTMDGSIKRLYDEGTITGE
                     EAYMNAYDKSKFEAIKDS"
BASE COUNT          371 a          677 c          719 g          431 t
ORIGIN      
        1 aacttggcga tgcgcgtgcc gagcagcggt tgcgcggcgt cttgccagcg gagctcgccg
       61 ccccccagca gcttttcggc gcgggcggcc aaagccggca cgacgggctt caaataaacg
      121 atcagcgcgc ggaatagatt cacgccttgc gtgcacacgg cgacgacttc ggcttccgcg
      181 ccgggctgct ttgcgagaac ccacggcttg tgctcgtcga tgtactgatt ggcccgatcg
      241 gcgagggcca tgatcttgcg gatcgcgcgc gagtactcgc gcccctcgaa gtccgcggcg
      301 agatcgtcgg ccgcggctgc aaactcgcga tagagcgcgg gctccggcaa ctcggcagcc
      361 aaccgcccgc cattgagctt ctcgatgaat ccggcgcagc ggctcgcgat gttgacgagc
      421 ttgccgacga ggtcggagtt cactttggcg atgaagtcgg cgaagttgag gtcgaggtcg
      481 tcgatcgtcg gcccgagctt cgacgcgtag tagtagcgca agtagtcggg atcgatcgct
      541 ttcgcgaaat cggcggcagt cacggagtta ccgcggcgct tcgaaatctt ttgtccgtcg
      601 accgtgacgt gcccgtgcac gaacaagccg ctcggtttgc ggtagcccgc gcccgagagc
      661 atcgcgggcc aaaagagcgc gtgaaagtaa gggatgtcct tgccgatgaa gtgatagagc
      721 tcgtgccggc tgtcggcacc ccaaaactcg tcgaagtcga tgccttcgcg tttgcagagg
      781 ctcaagaagc tcgccatgta gccgatcggc gcatcgaacc agacgtagaa gaacttgtcg
      841 cgctcgccgg ggatgcggaa accgaagtag ggcgcatcgc gcgagatgtc ccaatccttg
      901 agcccggctt tgaaccactc gtcgagcttg cgcgcgagca cggcgtcgac gtgctcgggc
      961 acccacttct cgagctcggc tgtgaacgcg ctgagcttca ggaacaagtg ctcggactcg
     1021 cgcacggacg gcttcgtgcc cgacaccgtg gagaccgcat ctttgagctc gagcggcgag
     1081 taggtggcgc cgcagctctc gcacgagtcg ccgtactgat cgggtgtgcc gcagttaggg
     1141 cacgtgcctt tgacgtagcg atccggcaag aacatctgcc gctcgacgtc gtaggcctgc
     1201 ttgatcgttt tgcgcgtgat gaagccgccg gccgcaagcc gccggtaaag ctcggtcgtg
     1261 agctgctcgt tctccggcga atgcgtcgtc aggtagttgt cgacgctgat gcgataggtc
     1321 gcgaagtcgc gcttgtgctc ctcggtcacg ccagcgacga gcgcctcggg cgtcgtgcct
     1381 tcctgctcgg cgcgcagcat cgtcggcgtc ccgtgcgcat cgcttgcgca gacgtatagg
     1441 cagcgatggc cgcgcagtcg ctggaaacgc acccaaacgt cggtctgcac gtactcgacg
     1501 acgtgtccca ggtgcaacga gctgttcgcg tacggcagtg cgctcgtaac gaggatggtg
     1561 cgcttgacgc tcaagactta cggggctccg cactgtaggt cggcagcgtc cgttgctgcc
     1621 gcgggcgcca ttatgccatt ggcgtcgctg ctgcggcgag gtcaggagtc tttgatggcc
     1681 tcgaacttcg acttgtcgta ggcgttcata tacgcctctt cgccggtgat cgtgccttcg
     1741 tcgtacagcc gcttgatcga gccgtccatc gtgcacatgc cttcgcgacc gctcgattgc
     1801 atgacgtttt cgagctgctc gagcttgcct tggcggatca ggttggcggc cgcagacgtg
     1861 ttgatgagga tctcgagggc tgcgacgcgg cccgtgccgt cctttttctt caagagctgc
     1921 tgcgagacga taccgcgcag cgacgtcgac agcatgttgc ggacgtgatc ctgcttgtcg
     1981 gctggaaaaa tatttacgac gcggtcgatc gtggccgccg caccattcgt gtgcagcgtc
     2041 cccatgacga gcacgcccat ctcggcggcc gtcatcgcga tacccatcgt gtcgaggtcc
     2101 cggagctcac cgacgagaat ggcgtctgga tcttcacgca gagccgaatg cagtgcgtgc
     2161 gcgaagctcg gactgtgcac gccgacctcg cgctggct
//
LOCUS       DATLEE010000056        13941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9721360, whole genome shotgun sequence.
ACCESSION   DATLEE010000056 DATLEE010000000
VERSION     DATLEE010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 13941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13941
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9721360"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VM692_01145"
     CDS             <1..438
                     /locus_tag="VM692_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50793.1"
                     /translation="LDLVPVAVRRNDAGRVGSLTLDVAALPTETQRAAELGSEAACPL
                     PDQWQAMQVFDVLAHNDARTPETMRYGPADLSLVLADNEDLFGTATDVPDYLRRTSIE
                     VSAYLAERLRMLDEPALARSVGDVLDEPRRAALLARRDRLLAR"
     gene            complement(446..1048)
                     /locus_tag="VM692_01150"
     CDS             complement(446..1048)
                     /locus_tag="VM692_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUQ50794.1"
                     /translation="MHATKASINGYKVRLLLSMLRLQYELVELDMYAGEHKREPFLTL
                     NPFGQMPALTDGDFTIADSHACLVYLARKYDPKGTWLPADAEGEAKVAEWLSKSANEV
                     HQGPWMKRAKIRRPDAIKLSNEEIDARCDHILKLMDAELGLREWLALGRPTIADISCF
                     GPISMLKVSGYDTDKWPSVTRWLDRIKALPGAHDIDGNPF"
     gene            1168..2085
                     /locus_tag="VM692_01155"
     CDS             1168..2085
                     /locus_tag="VM692_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUQ50795.1"
                     /translation="MALKPVTKATLQIHFCVLLWGFTAILGKLISLAALPLVWWRMLI
                     VVATLLLVPRVLRSLRNLPARLWLAFGGVGGLVALHWLTFYGAIKLSNASVAATCIAL
                     ASPFTAIVEPWVTRTKFSPRDLALGVAVLPGVALVVGGVPSGMHRGIAVGAVSALLVA
                     VFGAMNKRLAHRGDALMATALELGSGVLVLSMLAPVLPALLPELAGPVLAWPSPRDGI
                     LLATLAFACTLLPFSLSFVALRHISAFSAQLAVNLEPVYAIVLAIVLLGEQRELTPQF
                     YVGVAIIVGAVSVRPLLGRTREVSEIAGA"
     gene            complement(2093..3310)
                     /locus_tag="VM692_01160"
     CDS             complement(2093..3310)
                     /locus_tag="VM692_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50796.1"
                     /translation="MLLVFARAACGADASALFAQGEQAFAAGDHGEALRLFEAAREAG
                     SVGPSSYYNIGVCQYLLHDYDAAEHTFATLAAEFPAMRELAEYNRGLALRADGKVTAA
                     QQAFARARSSDDEKIVALANAQLRELGLSPTQAEPSWQAYVSGGLGYDDNVALVDDLV
                     LTAGGSSSSALTEMLGVISHDFGAAPLRVDATGYWIRYPSASEFDQSALRVALVARRE
                     LGMWTLTAGPTLSRTTLDGDGFEEAIGADLRLRRYFGADVSVDVRVVYDDVDAGAAQF
                     AYLDGSRRQLRFGVQHTGNGRLRLGYDLERNERADAGVSAARQRWSVAYSWSLPAEWT
                     ADAALTQRTSRYSQASVPRTERLSDVSFTANRELPMGWTLSPSYRWSNNDSSVKAFSY
                     EGQRVALGLSRSF"
     gene            complement(3423..3998)
                     /locus_tag="VM692_01165"
     CDS             complement(3423..3998)
                     /locus_tag="VM692_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50797.1"
                     /translation="MLTAAIVFAAQSQETPEANLEVTIRLLPQNAVGPGEITRRIELP
                     PVDAAPQVEPREAPPPAAESNGRGRDRDAVPAPSERGGDVAPAASERGRDIGPAPSER
                     GRDVAPAPAERGRDVVPEAGRGNDVAQDARERGREAGQETAEQAKENREIAGRPDNPG
                     RPDDPGRPGGPPGRPDDTGRPDDKGPPPGQK"
     gene            complement(4112..4747)
                     /locus_tag="VM692_01170"
     CDS             complement(4112..4747)
                     /locus_tag="VM692_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50798.1"
                     /translation="MRCEDAIEQITARNARPGRDLRAATEHAESCADCRAALRALDAL
                     RPLRTELAPLPSDGAMERAIDLALAANPAQRYRRGFLSGMAAGAALAATLAAVAVGLW
                     LSAGDQDAPRAVPEVRIALNQPRDVTVTVESPEALADAEVRVELRGAVALEGYAGQRE
                     LRWMTDLDRGVNQLTLPVVALDASGGQVLVEVTHGDKRRAFVLDVRGGAAG"
     gene            complement(4749..5318)
                     /locus_tag="VM692_01175"
     CDS             complement(4749..5318)
                     /locus_tag="VM692_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUQ50799.1"
                     /translation="MGKQVVTPGGESESERFAALIRQHYDVLYRSAYRLTRSTVDAED
                     LVQEVCVRAFPRVNELESLDHPRGWLLRVLYRLFIDLKRRYERKHVRAMEENEEFVSA
                     EPGPAEEADRALYRWRLEDAWRYLNAEQRLLLALHDIEGYTLVEIHSMTGLKEGTIKS
                     RLHRARVRLGRLLQREDAGVAGAVSERGG"
     gene            5529..5891
                     /locus_tag="VM692_01180"
     CDS             5529..5891
                     /locus_tag="VM692_01180"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HUQ50800.1"
                     /translation="MTLTRTISVAAGVIAMTVSAGCSSGTGDSSSGRSTLSVSLMDAP
                     VEDVTAVFVRITSMWIKPTGRPAVQLALTGAPLDVNLMALTDTNAAVLINEAVIEPRS
                     YEWLAMHIAAQRGVRDSF"
     gene            6038..7867
                     /gene="typA"
                     /locus_tag="VM692_01185"
     CDS             6038..7867
                     /gene="typA"
                     /locus_tag="VM692_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HUQ50801.1"
                     /translation="MLPKERLRNIAIIAHVDHGKTTLVDKLLRQAGSLDSRVVIPERA
                     MDSNDLEKERGITILAKNTAIGWGDWRINIVDTPGHADFGGEVERVLSMVDGVLLLVD
                     AVDGPMPQTRFVTSKAFQHGFTPLVVINKIDRDGARPDWVLDQTFDLFDRLGASDKQL
                     DFPVIYASALKGYASQEPGARSGDMQPLFEAIVNHVPSPPVESEGPLQLQVSSLDYNS
                     YVGLMGIGRISRGVVKRNMPVTLVQRDGQERNAQLMELYGFDGLTRTQIPEAGAGDIV
                     VFSGIEDLKISDTVCARGQGEGLPPLQVDEPTITMTFQVNKSPFAGNEGKYLTSRQIR
                     ARLEQELQHNVALRVEETDDADKFRVSGRGELHLSVLIENMRREGYELAVSRPAVIER
                     VVDGVVHEPWEQLTVDFGDQYQGGVMTRLAERKGELISMMPDGRGRVRLEYRIPARGL
                     IGFRTEYLTTTAGTGLLYHIFDGYAPRAKGEIGQRINGALVSMVQGKALGYALFNLQE
                     RGALFIGHGVEVYEGMIIGIHSRDNDLMVNPTKGKQLTNIRTQSADENIVLTPPIKFS
                     LEQALEFIDDDELVEVTPTNVRLRKKLLTENERKRESRKAADG"
     gene            complement(7904..8236)
                     /locus_tag="VM692_01190"
     CDS             complement(7904..8236)
                     /locus_tag="VM692_01190"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HUQ50802.1"
                     /translation="MSSRSKSTSSADKRLADYDAVFAALDNAARRQILMILHFRGDSM
                     TAGEIADRFSCSWPTTSRHLRKLEGAGLVDVERAGREWIYRLNARRLRVAAEWLEWFD
                     APRPVAKT"
     gene            complement(8202..8531)
                     /locus_tag="VM692_01195"
     CDS             complement(8202..8531)
                     /locus_tag="VM692_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50803.1"
                     /translation="MVTSLCIYRVKPGSEGAFKKLLAKHWPTLKRVGLAADVPSTIYQ
                     GSEGEKQPIFVELLNWKDAHGAEHAHELPDVMAVWEPMGKLCEPRSGRPPMEFPLVEP
                     LKIDFER"
     gene            8669..9493
                     /locus_tag="VM692_01200"
     CDS             8669..9493
                     /locus_tag="VM692_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50804.1"
                     /translation="MSVRTIAPWTSLSVGLALALCAALCGAQQRPAGAAAPATLPPLF
                     LKEEWRQRERPANAGDDFVPEGGVTAAAVTNQALELKLYDPDAASIPGYLKNPPPRSV
                     ARDWGGPSCIQLAGFNQNPPPERVANGTRTDPPNLWTGVCHTAVAATLRDRSSYVDLT
                     GLARIRWVTRTSGFHVVRPVVKLADGNWFVGDYGEGAPSANSTLFLESEFAVANVRWL
                     PLDISRVVTTGQTWATPDLSRVDEIGFADLLPGSGHGWGGFVNLGRIEVYGKRVAR"
     gene            complement(9526..10074)
                     /locus_tag="VM692_01205"
     CDS             complement(9526..10074)
                     /locus_tag="VM692_01205"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HUQ50805.1"
                     /translation="MHSSVGLPSDLAVDRVRLERPALRHAQQFLAAVRRSRELHGRWV
                     SPPATRRSFAEYVRSLRSDKRVGLLVLTDTDDLVGVINVNEIVRGSFQSAYLGYYAFQ
                     PFAGRGSFRAGLRLAIAYCFGDLELHRVEANIQPDNARSIALAKSLGFQKEGFSPLYL
                     KIAGRWRDHERWALLADNWRTR"
     gene            complement(10228..10893)
                     /locus_tag="VM692_01210"
     CDS             complement(10228..10893)
                     /locus_tag="VM692_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50806.1"
                     /translation="MRPLNVGVMRHKGLIVLSLAATLIAAWFSIHRTKRVSPQAAQSV
                     GDQAVARLVVPSEQRSILREPTAQSESERAVGTVQIAEPAGQDLASDRDSEHAADPSA
                     ALAERRASMNAAAAKASALAPLFMSASRDRTWAETAEAELSSRIAQVAGLELTTLRIE
                     CRETVCRIDFTFPSPEYLETSGGEMAMAAVNGTPGYETGGLILVGNDGTLTHYVPLRE
                     LGD"
     gene            11234..11905
                     /locus_tag="VM692_01215"
     CDS             11234..11905
                     /locus_tag="VM692_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015378144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HUQ50807.1"
                     /translation="MNIELTANEARVIGCLMEKSVITPDQYPLSLNALMNACNQKSSR
                     EPVMSLEQGVVQRTVNDLIAKKLVMTQEFGSRVDKYQQRFCNTHFAEIKFSPAEFAVI
                     TLLLLRGPQTPGELKSHSGRLHEFADGEADATIEALLTRADGPFVARLPREPRRKDHA
                     YTHLFGGPLESAPLSLPVEERGESPAPRPSALASIEARVAKLEDELAALKKALGLAQD
                     APPQG"
     gene            complement(11902..13140)
                     /locus_tag="VM692_01220"
     CDS             complement(11902..13140)
                     /locus_tag="VM692_01220"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ50808.1"
                     /translation="MQLRNLAVTVSSGLIALCATAVCAQAPNGAAASAAGSATTLDVQ
                     GGQIRVVTVAAGLLHPWSIALLPENGSMLVAERGQIRLIRNDSLVAEPVWSAAGVAAN
                     NDLKWITLHPRFASNQLVYLSYSKGGERGTTLAVARGKFDGRKLNDVKDVFVADAWEM
                     GGNIGGKILFGPDETLYVTVGDRDRLCCTGTEDNSLRMKAQSLSNHVGKTLRIRDDGS
                     VPPDNPFVGRAGAKPEIFTYGHRNGYGLAFNPETGVLWQAEIGPMGGDEVNILLPGHN
                     YGWPVVSMGRNYTGSLVSDKPYYQDGMDNARMFWVPSISPSSLVFYTGEAFANWRNNM
                     FVGALTGLSLQRIAFGQPSQAERREPVLRSLNLRIRDVQQSRDGYLFVATERSFGGSE
                     ANGSILRLEPVAATPSGPPR"
     gene            complement(13198..>13941)
                     /locus_tag="VM692_01225"
     CDS             complement(13198..>13941)
                     /locus_tag="VM692_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUQ50809.1"
                     /translation="TTVEVFASLVQAGTRDTVSVRKIAELAEATAQALGLSESQRRDV
                     HLAGLLCDLGKLTLPEALIHTPVAAMTAEQAHEFKQHTLNAERILLPLKPLNRVAAIL
                     RSHNERLDGAGYPDGLQGDAIPIESKVLHVVKDYDALLRRLIMPEALTPAEAVQYIND
                     YVGSPYDATVVAAFLKILTSVSKQGDNLRELRVSVAGLKPGMLVTRDLVNEHGVLMVP
                     RGRRLDSQAINSLQRLADGTAGFLLYVQR"
BASE COUNT         2390 a         4701 c         4456 g         2394 t
ORIGIN      
        1 ctcgatctcg tgccggtggc cgtgcgccgc aacgacgcag gccgcgtcgg ctccctgacg
       61 ctcgatgtcg cggcgttgcc gaccgaaacg cagcgcgcgg cggagctcgg cagcgaagcc
      121 gcgtgccctc tgcccgatca atggcaagcg atgcaggtgt tcgacgtgct ggcgcacaac
      181 gatgctcgaa cgcccgagac gatgcgctac ggcccggcgg atctatcgct cgtgctcgcg
      241 gacaacgagg acctgttcgg gacggcgacc gacgtgcccg actatctgcg ccgcacgagc
      301 atcgaagtgt cggcgtatct cgccgaacgg ctgcgcatgc tcgacgagcc tgcgctcgca
      361 cggagcgtcg gcgacgtgct cgacgagccg cgccgcgccg cgctgctcgc gcgccgcgac
      421 cggctgctcg cgcgctaacg cgtagctaga acggattgcc gtcgatgtcg tgtgcgccgg
      481 gcagcgcctt gatgcggtcc agccaccgcg tgacgctcgg ccacttgtcc gtgtcgtaac
      541 ccgacacctt gagcatcgaa atcgggccga agcacgagat gtcggcaatc gtcggccgac
      601 cgagcgcgag ccactcgcgt aacccaagct ccgcgtccat cagcttcaag atgtgatcgc
      661 aacgcgcgtc gatttcctcg ttcgacagct tgatggcatc gggccggcga atcttcgcgc
      721 gcttcatcca tggcccttgg tgcacttcgt tcgccgactt cgacaaccat tccgccacct
      781 tagcctcgcc ttctgcgtcc gcgggcagcc atgtcccctt tgggtcgtac ttgcgcgcga
      841 gatagacgag acacgcatgc gaatcggcga tcgtgaagtc gccgtccgtc agcgccggca
      901 tctggccgaa cgggttcaga gtcagaaacg gctcgcgctt gtgctcgccg gcatacatgt
      961 cgagctcgac gagctcgtac tgcaggcgca gcatcgagag cagcagccgg accttgtagc
     1021 cgttgatcga agctttggtt gcgtgcacgc gaatcatcgt cggttcctcg gtgggagcga
     1081 gatcactgta aagagcagcc tgcgcaagcg tcaatcgaca cgcagcgctt ttgcaaccgc
     1141 gagcggcgcc gccgacaata gcgcgccatg gcattgaagc cagtcacgaa agcgacgctg
     1201 cagatccact tctgcgtgct gctgtgggga ttcacggcca tcctcggcaa gctgatctcg
     1261 ctggccgcgc tgccgctcgt gtggtggcgc atgctaatcg tcgtcgcgac gctgttgctc
     1321 gtaccgcgcg tgctgcgctc gctgcgcaat ctgccggcgc ggctttggct tgcgttcggc
     1381 ggcgtcggcg gtctcgtcgc gctgcattgg ctgacgttct acggcgcaat caagctgtcc
     1441 aatgcatcgg tagccgcgac ttgcatcgcg ctcgcgtcgc cgttcacggc gatcgtcgag
     1501 ccgtgggtca cgcgcacgaa gttttcgccg cgcgatctcg cgctcggtgt cgcagttctg
     1561 cccggtgtcg cgctcgtcgt cggcggcgtg ccgagcggaa tgcaccgcgg catcgccgtc
     1621 ggtgccgtct cggcgctgct ggtcgcagtg ttcggcgcga tgaacaagcg gctcgcgcac
     1681 cgcggcgatg ctctcatggc gaccgcgctc gagctcggct cgggcgtgct cgtgctgagc
     1741 atgctggcgc cggtgctgcc tgcgctgctg cccgagctcg ccggaccggt gctggcatgg
     1801 ccgtcgccgc gcgacggcat cctgctcgcg acgctcgcgt tcgcttgcac gctgctgccg
     1861 ttctcgctgt cgttcgtcgc gctacggcac atcagcgcgt tctcggcgca gctcgccgtc
     1921 aacctcgagc ccgtctacgc aatcgtgctg gccatcgtgc tgctcggtga gcagcgcgag
     1981 ctcacgccgc agttctacgt cggtgtggcc atcatcgtcg gggccgtgtc ggtgcggccg
     2041 ctgctcggcc ggacgcgcga ggtcagcgaa atcgcaggag cttagcggcg tgctagaagc
     2101 tccggctcaa gccgagcgcc acacgctgcc cttcgtacga gaacgctttg acgctcgagt
     2161 cgttgttgga ccagcgatag ctcgggctca gcgtccaacc catcggcagc tcccgattgg
     2221 ccgtgaacga cacgtccgac aaacgctcgg tgcgcggcac gctcgcctga ctgtagcggc
     2281 tcgtgcgctg cgtcagcgca gcgtcggcgg tccattcggc aggcagcgac catgaatatg
     2341 cgaccgacca tcgctgtcgt gccgccgaga ctccggcgtc cgcgcgctcg ttacgctcga
     2401 ggtcgtagcc caggcgaagc cgaccgttac cggtgtgctg cacaccgaaa cgcagttgcc
     2461 gtcgcgagcc gtcgagatac gcgaactgcg ccgcccccgc gtcgacgtcg tcgtagacga
     2521 cgcgcacgtc gaccgagaca tcggcgccga aatagcggcg caggcgcagg tcggcgccga
     2581 tggcttcctc gaaaccatcg ccgtcgagcg tcgtacgaga aagcgtcggg ccggccgtca
     2641 gcgtccacat tccgagctct cttcgtgcga ccagcgcgac gcgcaaggcg ctctgatcga
     2701 actccgacgc gctcggatag cgaatccagt aacccgtcgc atcgacgcgc agcggcgcag
     2761 caccgaagtc gtggcttatt acgccgagca tttccgtgag cgcgctcgat gacgatcctc
     2821 cggccgtcaa gacgagatcg tcgacgagcg ccacgttgtc gtcgtagccg agaccgccgg
     2881 aaacgtaggc ttgccaggac ggctccgctt gcgtgggcga gagcccgagc tcccgcaatt
     2941 gcgcgttggc gagcgcgacg atcttctcgt cgtcactcga gcgcgctcgt gcgaaggctt
     3001 gctgcgccgc ggtgactttg ccgtcggcgc gcagcgcgag gcctcggttg tattcggcga
     3061 gctcgcgcat ggcgggaaac tccgcggcca gcgtcgcgaa ggtgtgctcg gccgcgtcgt
     3121 agtcgtgcag cagatactga caaacgccga tgttgtagta gctgctcggt cctaccgatc
     3181 ctgcctcacg cgccgcctcg aacaggcgca gcgcttcgcc gtggtcgccg gcggcaaagg
     3241 cttgctctcc ttgagcaaac agcgcgctgg catcggcgcc gcacgccgcg cgagcgaaaa
     3301 ccaatagcat gcaggcgacc gtgagcggag cccgaagcaa tcgtcaccca ggaacagtgg
     3361 aacgtaccct ttagtgtaca agggcactac gaccggcgac tcgcgggccg tgtagaggcg
     3421 cgctacttct gacccggagg tgggcccttg tcgtcggggc ggccggtgtc gtcggggcgg
     3481 cccggagggc cacccgggcg gcccgggtcg tcgggccgac ccgggttgtc aggacggccc
     3541 gctatttcgc gattttcctt ggcctgctca gcagtttctt gaccggcctc gcgaccgcgc
     3601 tcgcgagcgt cctgcgccac gtcgttgccg cgaccggctt cgggaaccac gtcgcgaccg
     3661 cgctccgccg gcgcgggtgc gacgtcgcgg ccccgctcgc tcggggcggg cccgatgtcg
     3721 cggccccgct cgctcgcagc gggcgctacg tcgccgccgc gctcgctcgg agcgggcacc
     3781 gcatcgcgat cacgacctcg gccgttgctc tcggctgccg gcggcggcgc ctctctgggc
     3841 tcgacctggg gcgctgcatc gaccggcggc aactcgatcc ggcgcgtgat ctcgccgggc
     3901 ccgacggcgt tctggggcag cagccggatc gtcacctcga gattggcctc gggcgtttcc
     3961 tgtgattgcg cggcgaagac gatcgctgcc gtgagcaccg cacacgccgt gcgcgcaacc
     4021 cgcccattca tcgtcgtagc catgctgttt ccctcccgcc ggtactacac gtgcaatccg
     4081 acgttcggtt ccctctctct ttcaataatc gtcaacccgc tgccccaccg cgaacatcga
     4141 gtacgaacgc gcggcgcttg tcgccgtgcg tcacctcgac gagcacctgc ccgccgctag
     4201 cgtcgagcgc aacgacgggc agagtcaact gatttacgcc gcgatcgaga tcggtcatcc
     4261 aacgcagctc gcgttgcccg gcataaccct cgagcgccac ggcgccgcgc agctcgacgc
     4321 gcacttcggc gtccgcgagc gcttccggcg actcgacggt gaccgtgacg tcgcgcggct
     4381 ggttcagagc gatgcgcacc tcgggcacgg ctcgcggcgc atcctggtca cccgcgctca
     4441 gccagagtcc gacggcgacg gccgccaacg ttgcggcgag cgccgcgccg gcggccatgc
     4501 cgctcaaaaa accgcgccga taacgctggg cgggattggc ggccaaggcg agatcgatgg
     4561 cgcgctccat ggcgccgtcg ctcggcagcg gagccaactc cgtgcgtagc ggtcgcaatg
     4621 cgtcgagcgc gcgcaacgcc gcacgacagt cggcgcaact ttcggcgtgc tccgttgccg
     4681 ccctgagatc gcgacccggc cgcgcgttgc gcgccgtgat ttgctcgatt gcgtcctcgc
     4741 atcgcatctc agccaccccg ttcactcacc gcgccggcaa cacccgcgtc ttcgcgctgc
     4801 aacaatcggc cgaggcgaac acgcgcgcgg tgcaaccgcg acttgatcgt gccttccttc
     4861 aagcccgtca tcgagtggat ctcgaccaac gtataacctt cgatgtcatg caaggccaat
     4921 aacagacgtt gctccgcatt caaataacgc cacgcgtcct cgaggcgcca gcggtacaac
     4981 gctcggtcgg cctcttcggc ggggcccggc tccgcgctga cgaactcctc gttctcctcc
     5041 atcgcccgca cgtgcttgcg ctcgtagcgg cgcttcagat cgatgaacag ccgatacagc
     5101 acgcgcaaca accagccgcg cgggtggtcg aggctctcga gctcattgac gcgcgggaag
     5161 gcccgcacgc agacctcttg caccaaatcc tcggcgtcca ccgtggaacg cgtcagccgg
     5221 taggccgagc ggtacagcac gtcgtagtgc tgtcgaatca acgctgcgaa ccgttccgac
     5281 tccgactcac cgcccggggt gaccacctgc tttcccatgc ggtgactgtc ctgcgcctac
     5341 cgcgcctaac ttaccaacac ggatagccga cgtttcggtt ccggctgaat gcagccaacc
     5401 cgttgattgc gtgctgcggc tcgatgctgc cgatcattat gtcaatttac aggaactcgc
     5461 cgcgcgactg aggcgtgtaa agccccatga gacaggcgtg cgcccttcgg cgcgagtggc
     5521 atgcgggaat gacactgaca agaacgatca gcgtcgctgc gggcgtgatc gcgatgacgg
     5581 tgtcagcggg ttgctcgagc ggcaccggcg actcgtcgtc cggccgaagc acgctcagcg
     5641 tgtcgctgat ggacgcgccg gtcgaggacg tgaccgcggt cttcgtgcgc atcacgtcga
     5701 tgtggatcaa gcccaccggc cgccctgccg tgcaactcgc gctcacgggt gctccgctcg
     5761 acgtgaatct catggcgctc accgacacga acgccgccgt cctcatcaac gaagctgtca
     5821 tcgagccgcg ctcctacgag tggctcgcga tgcacatcgc cgcgcaacgc ggcgtgcgcg
     5881 attcgttcta ggctttccgc ccaaccgctt gcttagggag gatactggcc ccgcttcggc
     5941 ggggcctttt ttgcgcccct agccggttcg cgatggtatc gcggcggatt caccgtataa
     6001 tgcggccctt tttcccctcc cccttttaaa gcgcgtcatg ttgcccaagg aacggctgcg
     6061 aaacatcgcg atcatcgcgc acgtggacca cggcaagacg acgctcgtgg acaagctctt
     6121 gcgtcaagcg ggttcgctcg actcgcgcgt ggtgattccc gagcgcgcga tggactcgaa
     6181 cgacctcgag aaagagcgcg gcatcacgat cctcgcgaag aacaccgcga tcggctgggg
     6241 cgattggcgc atcaacatcg tcgacacgcc ggggcacgcg gacttcggcg gcgaagtcga
     6301 gcgcgtgttg tcgatggtcg acggcgtgct gctgctcgtc gatgccgtcg acggccccat
     6361 gccgcagacg cgcttcgtca cgagcaaggc gttccagcac ggcttcacgc cgctcgtcgt
     6421 catcaacaag atcgaccgcg acggcgcgcg gccggattgg gtgctcgatc agacgttcga
     6481 cctgttcgat cggttgggcg ccagtgacaa gcagctcgac ttcccggtca tctacgcctc
     6541 ggcgctgaaa ggatatgcct cgcaggagcc cggcgcgcgc tcaggcgaca tgcagccgct
     6601 gttcgaggca atcgtgaatc acgtgccgtc gccgcccgtc gagtccgaag gaccgctgca
     6661 gctgcaggtg tcgagcctcg actacaactc ctacgtcggc ctcatgggca tcggccgcat
     6721 cagccgcggc gtcgtcaagc gcaacatgcc ggtgacgctc gtgcagcgcg acggccagga
     6781 gcgcaacgcg cagctcatgg agctctacgg cttcgacggg ctcacgcgca cgcagatccc
     6841 ggaagccggc gccggcgaca tcgtcgtctt cagcggcatc gaggacttga agatctccga
     6901 tacggtttgc gcgcgcggcc aaggcgaagg cctgccgcct ctgcaagtcg acgagcccac
     6961 gatcacgatg acgttccagg tgaacaagtc gccgttcgcg ggcaacgagg gcaaatacct
     7021 cacgagccgc cagatccgcg cgcgcctcga gcaggagttg cagcacaacg tcgcgctgcg
     7081 cgtcgaggag accgacgacg ccgacaagtt tcgcgtgtcg ggccgcggcg agctgcacct
     7141 ctcggtgctg atcgagaaca tgcgccgcga aggctacgag ctggccgtgt cgcggcccgc
     7201 cgtcatcgag cgcgtcgtcg acggtgtcgt gcacgagccg tgggagcagc tcaccgtcga
     7261 cttcggcgac caatatcaag gcggcgtcat gacgcgcctc gcggagcgca aaggcgagct
     7321 catctcgatg atgccggacg gccgcggccg cgtgcgcctc gagtatcgga tcccggcgcg
     7381 cggcctcatc ggcttccgca ccgaatacct gacgacgact gcgggcaccg gcctgctcta
     7441 tcacatcttc gacggctacg cgccgcgtgc gaaaggcgag atcggtcaac gcatcaatgg
     7501 cgcgctcgtc tcgatggtgc aaggcaaagc gctcggttac gcgctgttca acttgcagga
     7561 gcgcggcgcg ctattcatcg gccacggcgt cgaagtttac gaaggcatga tcatcggcat
     7621 ccacagccgc gacaacgacc tcatggtcaa cccgaccaag ggcaagcaac tcaccaacat
     7681 tcgaacccaa agcgccgacg agaacatcgt cctgacgccg ccgatcaagt tctcgctcga
     7741 gcaggcgctc gagttcatcg acgacgacga gctcgtcgag gtcacgccga cgaacgtgcg
     7801 cctgcgcaag aagcttttga ccgagaacga gcgcaagcgc gagtcgcgca aagccgccga
     7861 cggctagcgc actgagcgac cggccggcac cccgcccgaa ggtttacgtt ttcgccacgg
     7921 ggcgcggcgc gtcgaaccac tcgagccatt ccgccgcgac gcgcaagcga cgcgcgttga
     7981 gccgatagat ccactcgcgg cccgcgcgct cgacgtcgac gagccccgca ccctcgagct
     8041 tgcgcaagtg ccgcgacgtc gtcggccacg agcacgagaa acgatcggcg atctcgccgg
     8101 ccgtcatgct gtcgccgcga aagtgcagaa tcatgaggat ctggcgccgc gccgcgttat
     8161 cgagcgctgc gaacacggcg tcgtagtccg cgaggcgctt gtcagcgctc gaagtcgatt
     8221 ttgagcggct cgacaagcgg gaactccatc ggcgggcgcc cggagcgcgg ctcgcagagc
     8281 ttgcccatcg gctcccagac ggccatgacg tcgggcagct cgtgcgcgtg ttcggcgccg
     8341 tgtgcgtctt tccagttgag cagctcgacg aagatcggct gcttctcgcc ttccgaacct
     8401 tgatagatcg tcgacggcac atcggccgcg agcccgacgc gcttcaatgt cggccaatgc
     8461 ttggccaaaa gcttcttgaa cgcgccctcc gacccaggct tcacgcgata aatgcacagc
     8521 gaagtgacca ttcttcctcc ggtcttttgt tagccgttac gctaagcaat atacttagcc
     8581 aaatggctaa ctgcaacccg ggctgaaatt gggctcgatc ggccgtacac tggcgccgca
     8641 gcggccttgg cgccgttggg gagagctcat gtcggttcgc acaatcgcgc cgtggacgag
     8701 cctatcggtc ggtttggcgt tagcactctg cgcagcgctc tgcggcgctc aacaacggcc
     8761 agccggcgcg gccgcgcccg caacgctgcc gccgctgttt ctcaaggaag agtggcgaca
     8821 acgcgaacgc cccgccaatg ccggcgacga ttttgtgccg gagggcggag tcacggcggc
     8881 agccgtcacg aaccaggcac tcgagctcaa gctctacgat ccggatgccg cgagcattcc
     8941 gggctatttg aagaatccgc cgccgcgctc ggtggcccgc gattggggcg gcccgtcgtg
     9001 catccagctg gccggcttca atcagaaccc gccgcccgag cgtgtggcga acggcacccg
     9061 aaccgatccg ccgaatctgt ggaccggcgt ctgccacacg gccgtcgccg cgacgctgcg
     9121 cgaccgctcg agctacgtcg acctcacggg cctcgcgcgc atccgctggg tgacgcgcac
     9181 gtcgggcttt cacgtcgtgc ggcccgtcgt gaagctcgcg gacggcaact ggttcgtggg
     9241 ggactacggc gaaggcgcgc cgtcggcgaa ctcgacgctg ttcctcgagt cggagttcgc
     9301 cgtcgcgaac gtgcgttggc tgccgctcga catctcgcgc gtcgtcacga cgggacaaac
     9361 ctgggcgact ccggacttga gccgcgtcga cgagatcggc ttcgcggatc tgttgccggg
     9421 aagcggtcac ggctggggcg gtttcgtgaa cctcgggcgc atcgaggtct acggcaagcg
     9481 cgtcgcgcgc tagatcgaaa gccgcgtagc cgtcaggcgg cctcttcagc gtgtgcgcca
     9541 gttatccgct agcagcgccc agcgttcgtg gtcgcgccag cgccccgcga tcttgaggta
     9601 gagcggcgag aatccctctt tctgaaaacc aagactcttg gccagcgcga tcgagcgcgc
     9661 gttatccggc tggatgttcg cttcgactct gtggagctcg aggtctccga agcagtaggc
     9721 aatcgccaag cgcaagcctg cgcgaaagga gccacggccg gcgaacggct gaaaagcgta
     9781 gtagccgagg tacgctgact ggaagctgcc gcgcacgatt tcattcacat tgatcacgcc
     9841 gacgaggtcg tcggtgtcgg tcagaaccaa gagacccact cgcttatcgc tgcggaggct
     9901 tctcacgtac tcggcgaagc tgcgtcgagt ggccggcggc gaaacccaac gtccatgcag
     9961 ctctcgactg cgacggacgg ccgcaaggaa ttgttgtgcg tgacgaagtg caggccgctc
    10021 cagtcggacg cgatcgaccg caagatcgct tggtagaccc acgctgctat gcactacggt
    10081 cgagatcagg cgtctgagcg agacgaattg acgcgttcat ggcgatgcct tcaaacatac
    10141 gctcaacacg ctttccggcg acgcacaccg ccgtatgagc gcgcccgacg ccgtatcgcc
    10201 gtccgctgca cacactcatt cgctgatcta atcacctaat tcgcgcagcg gaacatagtg
    10261 ggtcagtgtt ccatcattgc cgaccaagat caggccaccg gtctcgtacc ccggggtgcc
    10321 attcacagcg gccatcgcca tttcgccgcc cgaggtctcc aagtactccg gactcggaaa
    10381 tgtgaaatcg atgcggcaaa ccgtctcgcg gcattcgatg cgcaaggtcg tcagctcaag
    10441 acccgctacc tgcgcgattc gactgctcaa ttcagcttcg gccgtctccg cccacgttcg
    10501 atccctcgac gcggacatga acaatggcgc aagagcgctc gccttggctg cagccgcgtt
    10561 cattgatgcc ctccgttcgg ctagcgcagc gcttggatca gcagcgtgtt cggagtcacg
    10621 gtccgacgct aaatcttgac ccgccggctc cgcaatttga acggtgccga ccgcgcgctc
    10681 actctccgac tgcgcggtgg gctcgcgcag aatagaacgc tgctcgcttg gcacaacgag
    10741 tctcgcgaca gcctgatctc cgaccgattg agctgcctgc ggcgaaacac gcttcgttcg
    10801 gtgtattgaa aaccacgcgg caatcagcgt cgcagctaaa gaaaggacga taaggccttt
    10861 atgccgcata acgccgacgt tcagcggccg cacgctgcgc tatccagccc gccgcgtgtg
    10921 cacaatgagg tgacgcgcct agcaccacca tggacctctg agagttcaga tcctcgaggc
    10981 gccaataggt aatgccgctt agataactga gaacgaagag agcgagcaag actggtccga
    11041 gccgccgtgc cgccggcgaa cctcgaaaat tggagttcat ggccgtaacc tcctagccgg
    11101 tgcgccatct acgtggcgcc taaccccgca gttcagcacg ctgctcggtc gagggcggtg
    11161 catcggaagc cacgtgctac ctggcgacga tgtcgtatat tccagctttc gaataaacgc
    11221 aacggacacg caattgaaca tcgagctcac cgcgaatgaa gcccgcgtca tcggctgtct
    11281 catggagaag tccgtcatca caccggatca gtatcccctg tcgttgaacg cgctgatgaa
    11341 tgcgtgcaac cagaagtcga gccgcgagcc cgtcatgtca ctcgagcaag gcgtcgtgca
    11401 acgcaccgtc aacgatttga tcgcgaagaa gctcgtgatg acgcaggagt tcggcagccg
    11461 cgtcgacaag tatcaacagc ggttctgcaa tacgcacttc gcggagatca agttctcgcc
    11521 ggccgagttc gccgtcatca ctctgttgtt gctgcgtggc ccgcaaacac cgggcgagct
    11581 caaatcgcac agcggccgcc tgcacgagtt cgccgacggt gaagcggacg cgacgatcga
    11641 agcgctgctg acgcgtgccg atgggccgtt cgtcgcgcgc ctgccacgcg agccgcggcg
    11701 caaggatcat gcgtacacgc atctcttcgg tgggccgctc gaatccgcgc cgctgtcgct
    11761 gccggtcgaa gagcgcggcg agtcgccggc gccgcgcccg agcgcgctag catcgatcga
    11821 ggcgcgcgtg gcgaagctcg aggacgagct cgccgcgctg aagaaggcgc tcggtttagc
    11881 gcaggacgcg ccgccgcaag gctagcgagg cggccccgac ggcgtggccg cgacgggctc
    11941 gagccgcaag atcgagccgt tcgcctcgct gccgccgaag ctgcgctccg tcgcgacgaa
    12001 gagatagccg tcgcggctct gctgaacgtc acggatgcgc aagttcaacg agcgcagcac
    12061 gggctcgcgg cgctcggctt gcgatggctg accgaacgcg atgcgctgca agctcaaccc
    12121 ggtcaacgcg ccgacgaaca tgttgttgcg ccaattggcg aatgcttcgc ccgtgtagaa
    12181 caccaggctc gacggactga tcgacggcac ccagaacatg cgcgcgttgt ccatgccgtc
    12241 ctgatagtac ggcttgtctg aaaccagcga gcccgtgtag ttgcggccca tcgagacgac
    12301 gggccaaccg tagttgtgac cgggcagcag gatgttgacc tcgtcgcctc ccatggggcc
    12361 gatctcggct tgccagagga cgcccgtttc ggggttgaac gcgaggccgt agccgttgcg
    12421 gtgcccgtac gtgaaaatct cgggcttggc gcccgcgcgg ccgacgaacg gattgtcggg
    12481 cggcacgctg ccgtcgtcgc ggatacgaag cgtcttaccg acgtgattgc tcaagctctg
    12541 cgccttcatg cgcaggctgt tgtcctcggt ccccgtgcag cacagccgat cgcggtcgcc
    12601 gacggtgacg tacaacgtct catctgggcc aaacagaatc ttgccgccga tgttgccgcc
    12661 catctcccac gcatcggcga cgaagacgtc cttcacgtcg ttgagcttgc gaccgtcgaa
    12721 tttaccgcgc gcgacggcga gcgtcgtgcc gcgctcaccg cccttggagt acgagagata
    12781 cacgagctga ttcgaagcga agcgcggatg cagcgtgatc catttgagat cgttgttcgc
    12841 cgcaacgccg gcggccgacc acacgggctc cgcgactagc gagtcgttgc gaatcagccg
    12901 tatttggccg cgctcggcga cgagcatcga gccgttctcg ggcagcagcg cgatgctcca
    12961 tgggtgcaga agcccagcag ccaccgtgac gacgcgtatt tggccgccct gcacgtcgag
    13021 ggtggtagcc gacccggccg cgcttgccgc ggcgccgttc ggcgcttggg cgcaaacggc
    13081 cgtcgcgcaa agtgcaatga gacccgatga gacggttacc gcgagattgc gcagctgcat
    13141 ggacgtcccc ccgtcaaacg acgcgaagat cgtagcatgc gtcgccgcga acggcgtcta
    13201 gcgctggacg tacagcagga agccggccgt gccgtccgcg aggcgctgca gcgagttgat
    13261 agcttggcta tcgagtcgcc ggccgcgcgg caccatgagg acgccgtgct cgttgacgag
    13321 gtcgcgcgtg accagcatgc cgggcttcaa gccagcaacc gacactcgaa gctcgcgcag
    13381 gttgtcgccc tgcttcgaaa cggatgtgag gatcttcagg aaagcggcga cgacggtcgc
    13441 gtcatagggg ctgccgacgt aatcgttgat gtactgcacg gcttcggccg gcgtcagcgc
    13501 ctcgggcatg atcaaccgcc gcaacagcgc gtcgtagtcc ttgacgacgt gcagcacctt
    13561 cgactcgatc gggattgcgt cgccctgtaa cccgtcggga taacccgcgc catcgagccg
    13621 ctcattgtgc gagcgcaaga tcgcggctac tcgattgagc ggcttcaggg gcaacaagat
    13681 gcgctcggcg ttcagcgtgt gctgcttgaa ctcgtgagct tgctccgcgg tcatcgcggc
    13741 gaccggcgtg tggatcagtg cctcgggcag cgtcagcttg ccgaggtcgc agagcaggcc
    13801 ggccaagtgc acgtcgcgcc gctgactttc ggagagcccg agcgcctgcg ccgtggcctc
    13861 cgcgagctcc gcgatcttgc gaacgctgac cgtgtcgcgc gtgccggctt ggacgaggct
    13921 cgcgaacact tcgacggtgg t
//
LOCUS       DATLEE010000057         5601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10467120, whole genome shotgun sequence.
ACCESSION   DATLEE010000057 DATLEE010000000
VERSION     DATLEE010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10467120"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VM692_01230"
     CDS             complement(<1..717)
                     /locus_tag="VM692_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016915979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HUQ50810.1"
                     /translation="MPFDPSTPYNDLPPLPPPLESIETAPLLKKCINARVALAELKQA
                     AELIPNAAVLVNALPLLEAQASSEIENIVTTTDRLLEFVDAPEGRADPATKEALRYRR
                     ALFEGVRKVQNGRITVETAIDICRTIKDETIDLRSKAGTTLRNIATAAVIYTPPVGKE
                     LIRGKLENWQQFLTARDDLDPLIRCAVQHYQFEAIHPFADGNGRTGRVLNSLFLVERG
                     LLTQPILYLSRYIIAHRADSY"
     gene            complement(931..3549)
                     /locus_tag="VM692_01235"
     CDS             complement(931..3549)
                     /locus_tag="VM692_01235"
                     /inference="COORDINATES: protein motif:HMM:NF023288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase domain-containing protein"
                     /protein_id="HUQ50811.1"
                     /translation="MSTQLSDSAWASLAKSLDVEEAALRAVATVESAGAGFLPGEPAR
                     PKILFEAHAFHRLTGGRFAPQAPDLSSPTWDRALYARTAAGEWKRLEAACTFDRPAAL
                     QSASWGLFQIMGFNYAYCGCPDVEAFVAKQYAGADDQLACFARFIARPPYLPALRNKD
                     WTKFAEAYNGPAQAKNKYAEKIAAAYERFAAMPAASQRPLTRRRRAAKPKATQLPPGR
                     PEFAPVPAMRRKNLHKRNVRPDAIDLRDWEYRPSIGFAPPDVLWPNDPRRVKQQGETN
                     ACTGFALATVLEYLLERSATKQAEDISAFMLYSMARRYDEWAEDENEDSGSSLRGALK
                     GWARHGASCERLWASLRMPPASNVPTEDWWLDAVKRPLGAYYRIAPDNVRDIHVALKE
                     VGAVYASALTHQGWDALLGRKRAPTPTDPDEIPVIDSARGAADQGHAFAIVGYTRKGF
                     IVQNSWGAQWGAGGFAVLPYADWLRNAMDCWVIQLGAVTAEHEVVSQAPSLRVERRAG
                     RDVAVVSSNETLADHEISPFVINMENEGRLSQRGRFRTNADDLRALLTNHLPVARARW
                     QLAADEPMDIAIYAHGGLTDEDAAAKTARDWVPHLYTNRIFPIFLMWETGAGKTLRNM
                     FEDVTRGEAELAAAGGRWERFKERFSEWKDERLEGLSRFPGGRMWGEMKQNAAALSGH
                     ADSGVVKLFALFKDAGVRAALPPVRLHLIGHSAGAIVHSFLGARALKQRLELESISLL
                     APAVRLDLFDGLLGAAIAASGIRVLIANLTDAAERNDDTCKPYGHSLLYLVSRSFEEH
                     EETPLLGMEKHLVPALATKAWSANVRQLRCPGGMWDGSSAATRATTHGGLDDDEAVRD
                     AVAAFVTARRTSRRPL"
     gene            3652..>5601
                     /locus_tag="VM692_01240"
     CDS             3652..>5601
                     /locus_tag="VM692_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ50812.1"
                     /translation="MRTQKSRATAFCLVAALAAGGAQAQNGRQQEKPYTTWRTYGGAG
                     DASQYSALNQINKSNVTQLTVAWSFPVGERSFVFNPVVVDNVMYVLARDNDIVALDAA
                     TGRELWSHPHEGPVTARGINYWQSADGNDRRLLYLNAGMLTALNARTGQTITTFGKNG
                     FVDLRDGLGAGGWNIVEVRPLHTSNPGRVIGNLMVVSLPAQGAGYASTPGDVQAYDVV
                     TGKLAWVFHSVPHEGEFGYDTWPKEGHATAGGVHNWSELTVDEANGIVFVPFGTARYD
                     FFGGNRAGNNLYANSLVAIDARNGKRLWHQQLVHHDLWDYDLPQAPKLLTIRDGRRTR
                     EVVAQATKHGFLFVFDRKSGEPIWPIEERAVPQSDVPGEHTSPTQPFPTKPEPFARQS
                     FTERDINPYLPAAEQDVLRQKLRSSRNEGLFTPPSFVGSIGMPGHNGGANWASSAVDP
                     INGELYIVSKNLPVMMRVEISDDEPSARTVNGPVVTREEAAKTLEAAKLRAAAAGSGV
                     RYSVPYDFLRSQTNGMGAFGAPFSELTAYDLNTGEIKWRVPDGGTIGIGDDVGAHFSR
                     GAPLVTAGGLVFAATAQDRKLRAYDRDNGKVLWSYTLPGGSEGIPATYQLDGRQYLAV
                     PVAAGAGLFAPLLDPAPPQPPLERAY"
BASE COUNT          946 a         1881 c         1857 g          917 t
ORIGIN      
        1 gtaggagtcg gcgcggtgcg cgatgatgta acggctcaga tacagaatcg gctgtgtcag
       61 taagccgcgt tcgaccagga aaaggctgtt cagcacgcga ccggtgcggc cgttgccgtc
      121 cgcgaacgga tggatcgcct cgaattggta gtgctgcacc gcgcagcgaa tcagcggatc
      181 gaggtcgtca cgcgccgtca ggaattgctg ccagttctcg agcttgccgc ggataagctc
      241 tttgccgacg ggcggcgtgt agatcacggc cgcggtggcg atgttgcgca gcgtcgtgcc
      301 ggctttcgag cgcaggtcga tcgtctcgtc cttgatcgtc cggcagatgt cgatcgcggt
      361 ctcgacggtg atgcgaccgt tctgcacctt gcgcacgcct tcgaacaacg cgcgccgata
      421 gcgcagcgct tccttcgtcg ccgggtccgc gcgaccttcc ggcgcgtcga cgaactcgag
      481 cagccggtcc gtcgtcgtga cgatgttttc gatctcggag ctcgcttgcg cttcgagcag
      541 cggcagcgcg ttgacgagca cggctgcgtt cggaatcagc tccgcggcct gcttgagctc
      601 ggccaacgcg actcgagcgt tgatgcactt ttttagcaat ggggccgtct cgatgctttc
      661 gagcggcggc ggcaacggcg gcaggtcgtt ataaggagtc gacgggtcga acggcatagg
      721 tacgaaatcc ccgaaaaatc gacacgccgt gaggatgtgt cgatggcctc gacgcgacgg
      781 gctgatatgt cgaaaagtca cagaaaaatc gacatattag ccgggtcacg tcgatgtcat
      841 cgacacgaag ccggaatatg tcgaaaagtc acctgttttt cgacatgtca gtataggccg
      901 gcggtgtgat ggagttccgt gacgggagtc tcagagcggc cgccgcgagg tccgacgcgc
      961 ggtgacgaac gccgcgacgg cgtcgcggac ggcctcgtcg tcgtcgagcc cgccgtgcgt
     1021 cgtggcgcgc gtcgccgccg aggacccgtc ccacattccg ccggggcagc gtagctgccg
     1081 cacgttggcg ctccaggctt tggtcgcaag cgccggcacg agatgcttct ccatgccgag
     1141 caacggcgtc tcctcgtgct cctcgaacga gcgcgagacg agatacaaca gcgaatggcc
     1201 atacggcttg caggtgtcgt cgttgcgctc ggccgcgtcc gtcaagttcg cgatcagcac
     1261 gcgtatgccg ctcgccgcga tcgcagcgcc gagcaacccg tcgaacaggt cgagccgcac
     1321 ggccggcgcc agcagtgaaa tcgactcgag ctcgagccgt tgcttgagcg cccgcgcgcc
     1381 aagaaacgaa tgcacgatcg cgccggccga gtgaccgatc agatgcaggc gaacgggcgg
     1441 cagagccgcc cgaacgcccg cgtccttgaa cagagcgaac agcttcacga cgcccgagtc
     1501 ggcgtgcccc gacagcgctg ccgcgttctg cttcatttct ccccacatgc gaccgcccgg
     1561 aaaacgcgac agcccctcga gccgctcgtc tttccactcc gaaaatcgtt ctttgaatcg
     1621 ctcccaacgc ccgcccgcgg ccgccagctc ggcctcgccg cgcgtgacgt cctcgaacat
     1681 gttgcgcagc gtcttgccgg cgccggtctc ccacatcaag aagatcggga agatgcgatt
     1741 cgtgtagaga tggggtaccc agtcgcgcgc ggttttcgcg gcggcgtcct cgtcggtcaa
     1801 accgccgtgg gcgtagatcg cgatgtccat cggctcgtcg gccgcgagct gccagcgcgc
     1861 acgcgcgacg ggcagatggt tcgtcaagag cgcgcgcaga tcgtcggcgt tggtgcgaaa
     1921 acggccgcgc tgcgagagcc gaccctcgtt ctccatgttg atgacgaacg gcgagatctc
     1981 gtgatcggcc agcgtctcgt tgctcgagac gacggcgacg tcgcgcccgg cgcgccgctc
     2041 gacgcgcagc gacggtgcct gcgacacgac ctcgtgctcg gccgtcacgg cgccgagctg
     2101 gatgacccag caatccatcg cgttgcgcag ccaatcggcg tagggcagca cggcaaagcc
     2161 gccggcgccc cactgcgcac cccacgagtt ctgcacgatg aagcccttgc gcgtgtagcc
     2221 gacgatcgcg aacgcgtgac cttgatcggc agcgccgcgc gccgagtcga tgacgggaat
     2281 ctcatcggga tcggtcggcg tcggggcgcg cttgcgaccc aacagcgcgt cccaaccttg
     2341 gtgcgtcagc gcgctcgcat agaccgcgcc gacttctttc agcgcgacgt ggatgtcgcg
     2401 cacgttgtcg ggcgcgatgc ggtagtaagc gccgagcggc cgcttcacgg cgtcgagcca
     2461 ccagtcttcg gtcggcacgt tcgaggcggg cggcatgcgc agcgaggccc agagccgttc
     2521 gcagctcgcg ccgtgccgcg cccaaccctt cagcgcgccg cgcagcgacg agccgctgtc
     2581 ctcgttctcg tcctcggccc attcgtcgta gcggcgcgcc atgctgtaga gcatgaacgc
     2641 cgagatgtcc tcggcctgct tcgtcgcgga gcgctcgagc aggtactcga gcaccgttgc
     2701 gagtgcgaag cccgtgcagg cattcgtctc gccttgctgc ttgaccctgc gtggatcgtt
     2761 cggccacaac acgtcgggcg gcgcgaaccc gatgctaggc cggtactccc agtcgcgcag
     2821 gtcgatggcg tcgggccgca cgttacgctt gtgcaaattc ttgcggcgca tcgcgggcac
     2881 cggcgcgaac tcggggcggc ccggcggcag ctgcgtcgcc ttgggcttgg ccgcacgccg
     2941 gcggcgcgtc agcggtcgct gcgaggcagc cggcatggcg gcgaaccgct cgtacgccgc
     3001 ggcgatcttc tcggcgtact tgttcttcgc ttgcgcaggt ccgttgtagg cctcggcgaa
     3061 cttcgtccag tccttgttgc gcagcgccgg caaatacggc ggccgcgcga tgaagcgcgc
     3121 gaagcacgcg agctgatcgt cggcacccgc atattgcttc gcgacgaacg cttcgacgtc
     3181 ggggcaaccg cagtacgcgt agttgaagcc catgatctgg aacaagcccc agctcgccga
     3241 ctgcaatgcc gcgggccggt cgaacgtgca cgcagcctcg aggcgcttcc actcgccggc
     3301 cgccgttctc gcatacaacg cgcgatccca cgtcgggctg ctcaagtcgg gcgcttgcgg
     3361 cgcgaaccgc ccgccggtca ggcgatggaa cgcgtgcgct tcgaacagga tcttcggccg
     3421 cgccggctca cccggcagaa acccggcgcc cgccgactcg acggtcgcga ccgcgcgcaa
     3481 cgcggcttct tcgacgtcga gggacttcgc gagactcgcc cacgccgagt cggatagttg
     3541 cgtgctcatg agcggctgta tagcccacgc ccagcgcacc gtcaatcaac ttcttgacac
     3601 tgcttgtgag ataaactggc cgcacaaaaa cgacggcacc cggggaagct catgcgcact
     3661 cagaaaagtc gggccaccgc cttctgtcta gtcgcggcct tggccgccgg cggcgcgcag
     3721 gcgcagaacg ggcggcagca ggagaagccg tacacgactt ggcgcacgta cggcggcgcc
     3781 ggcgatgcgt cgcagtactc ggcgctgaac caaatcaaca agtcgaacgt cacgcagctt
     3841 acggtcgcgt ggagctttcc cgtcggcgag cgcagcttcg tgttcaaccc cgtcgtggtc
     3901 gacaacgtca tgtatgtttt ggcgcgcgac aacgacatcg ttgcgctcga cgcggccacg
     3961 ggacgcgagc tctggtcgca cccgcacgaa ggccccgtga cggcgcgcgg catcaactac
     4021 tggcagagcg ccgacggcaa cgatcgccgc ctgctgtact tgaacgccgg catgctgacg
     4081 gcgctgaacg cgcgcacggg ccagaccatc acgacgttcg gcaagaacgg cttcgtcgac
     4141 ttgcgcgacg gcctcggcgc cggcggctgg aacatcgtcg aggtccggcc gctgcacacg
     4201 agcaacccgg gccgcgtcat cggcaacttg atggtcgtct cgctgccggc gcaaggtgcg
     4261 ggctacgcgt cgacgccggg cgacgtgcag gcctacgacg tcgtgacggg caagctcgcg
     4321 tgggtgttcc acagcgtgcc gcacgaaggc gagttcggct acgacacgtg gccgaaagag
     4381 ggacacgcga cggccggcgg cgtgcacaac tggagtgagc tgaccgtcga cgaagcgaac
     4441 ggcatcgtgt tcgtgccgtt cggcaccgcg cgctacgact tcttcggcgg caatcgcgcc
     4501 ggcaacaatc tgtatgcgaa ctcgctcgtc gcgatcgacg cacgcaacgg caagcggctc
     4561 tggcatcagc agctcgtgca ccacgacctc tgggactacg acctgccgca agcgccgaag
     4621 ctgctgacga tccgtgacgg ccgccgcact cgcgaggttg tcgcgcaggc gacgaagcac
     4681 ggcttcctgt tcgtgttcga tcgcaaatcg ggcgagccta tctggccgat cgaagagcgc
     4741 gccgtgccgc aatccgacgt gcccggcgag cacacgtcgc cgacgcagcc gttcccgacg
     4801 aaaccggagc cgttcgcgcg ccaatcgttc acggagcgcg atatcaatcc ctacttgccc
     4861 gcggccgagc aggacgttct gcggcagaag ctgcgcagct cgcgcaacga gggcttgttc
     4921 acgccgccga gcttcgtcgg ctcgatcggc atgccgggtc acaatggcgg cgccaactgg
     4981 gcgagctcgg ccgttgatcc gattaacggc gagctgtaca tcgtttcgaa gaacctgccc
     5041 gtgatgatgc gcgtcgaaat tagcgacgac gagccgtcgg cgcgcaccgt caacggccct
     5101 gtcgtgacgc gcgaagaagc agcgaagacg ctcgaggccg ccaagctgcg cgcggcagcc
     5161 gcgggcagcg gagttcgcta ctccgtgccg tacgacttct tgcgcagcca aacgaacggc
     5221 atgggcgcgt ttggcgcgcc gttctcggag ctgaccgcgt acgacttgaa cacgggcgag
     5281 atcaaatggc gcgtgcccga cggcggaacg atcggcatcg gcgacgacgt cggcgcacac
     5341 ttctcgcgcg gcgctccgct cgtgacggcc ggcggcctcg tgttcgcggc gactgcgcag
     5401 gatcgtaagc tgcgtgctta cgaccgcgac aacggcaagg tgctctggtc gtacacattg
     5461 ccgggcggct ctgaaggcat tcccgcgacg tatcagctcg acgggcgaca gtatctcgcc
     5521 gtgcccgtcg cggccggcgc aggcttgttc gcaccgctgc tcgatccggc gccgccgcaa
     5581 ccgccgctcg agcgcgcgta c
//
LOCUS       DATLEE010000058         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10467267, whole genome shotgun sequence.
ACCESSION   DATLEE010000058 DATLEE010000000
VERSION     DATLEE010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10467267"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1999)
                     /locus_tag="VM692_01245"
     CDS             complement(<1..1999)
                     /locus_tag="VM692_01245"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ50813.1"
                     /translation="MRRYVVGLAVLTASALGAAWWLGYQHDDAAAARDLLGRYCTDCH
                     NAADFTADLVIEPAKVDAIAADPEHWEKIVRKLRSETMPPEGPRPEHASYVRAATFLE
                     SQLDVAAASRPQPGDVPAFRRLTRTEYRNAIRDLLALDDLPTELDFNLLLPADNAASG
                     FDNIADLLFVSPVVMERYIAAAQKIARLAVGDLRMPVMVNIHQLSEQLPQDERIDGLS
                     FGTRGGLAVPTYLPLDAEYVVEVETAAAVTERHEIELSVDGRRVAVEAVDAQPAGERP
                     RRSSEQIAFRAPLAAGPHLLGITFVERSEALDESITRGRMRGRGTLPAIAMATIRGPY
                     DATGPGDTPSRNRIYVCRPSDASDDAACAEQILRTLAHRAYRRPVTDADLAELLPFYE
                     MGRAEGSFDTGIQFALERLLVSPQFLYRIEREPAEPGALPVSDIELASRLSFFIWSSI
                     PDDELLDAAENGSLREPGVLRAQVQRLLADARSESMVTNFAAQWLFLRDVGVKDPDIF
                     LFPDHDVSLRAALEHETELFVDSVLRGGGSVLELLTAKHTFVNERLAKHYGLPHVQGS
                     YFRRVELGDDSRRAGLLGQGSILTVTSYAARTSPVLRGKYVLDNLLASPPPPPPADVP
                     SLATEDAADGAALTLREAMIRHRANPQCASCHAKMDPIGFAL"
BASE COUNT          350 a          746 c          808 g          365 t
ORIGIN      
        1 cgagcgcgaa gccgatcggg tccatcttcg cgtggcagct ggcgcactgg gggttcgctc
       61 ggtggcgaat catcgcctcg cgcagcgtca aagccgcgcc gtcggcagcg tcctcggtcg
      121 ccagcgacgg cacgtcggcc ggcggcggcg ggggcggcga cgccagcaag ttgtcgagca
      181 cgtacttgcc gcgcagcacc ggagacgtgc gcgccgcgta cgacgtgacg gtcaagatgc
      241 tgccctgacc gaggagcccc gcgcggcggc tatcgtcgcc gagctcgact cgccggaagt
      301 agctcccttg cacgtgcggt aagccgtagt gcttcgcgag ccgctcgttg acgaacgtgt
      361 gcttcgcagt cagcagctcg agcacgctgc cgccgccgcg caacacgctg tcgacgaaca
      421 gctccgtctc gtgctcgagc gcggcgcgca gcgagacgtc gtggtcgggg aacaggaaga
      481 tgtccggatc tttgacgccg acgtcgcgca ggaatagcca ctgcgcagca aagttcgtca
      541 ccatcgattc agagcgcgca tcggccaaca gccgctggac ctgtgcacgc agcacgccgg
      601 gctcgcgcaa cgagccgttc tcggcggcgt cgagcaactc gtcgtcgggg atgctgctcc
      661 agatgaagaa cgacaagcgt gacgcgagct cgatgtcgct gacgggtaag gcgcctggct
      721 cggccggctc gcgctcgatg cggtagagga actgcgggct cacgagcaaa cgctcgagcg
      781 cgaattggat cccggtgtcg aagcttcctt cggcgcgccc catctcgtag aacggcagca
      841 gctccgcgag gtcggcgtcc gtcacggggc gccgataagc gcggtgagcc agcgttctga
      901 gaatctgctc ggcgcatgcc gcgtcgtcgc tggcgtcgct cgggcggcaa acgtagatgc
      961 ggttgcggct cggcgtatcg ccgggtcccg tcgcgtcgta aggcccgcga atcgtcgcca
     1021 tcgcgatcgc gggcaacgtt ccgcggccgc gcatccggcc gcgcgtgatg ctttcgtcga
     1081 gcgcttccga gcgctcgacg aacgtgatcc cgagcagatg cggtccggcc gcgagcggtg
     1141 cacggaacgc gatctgctcg ctgctgcgtc gtggccgttc gccggccggc tgcgcgtcga
     1201 ccgcttcgac tgcgacgcgc cgcccgtcga cgctgagctc gatctcgtgc cgctccgtga
     1261 cggcggcggc cgtctcgacc tcgacgacat actcggcgtc gagcggcaga tacgtcggca
     1321 ctgcgagccc gccgcgcgtg ccgaacgata agccgtcgat gcgctcgtcc tgcggcaact
     1381 gctccgataa ctgatgaatg ttgaccatga cgggcatgcg caggtcgccg acggcaaggc
     1441 gcgcgatttt ctgcgctgcg gcgatgtagc gttccatgac gacgggcgac acgaacagca
     1501 aatcggcgat gttgtcgaag ccgctcgcgg cgttgtcggc cggcaagagc aaattgaaat
     1561 cgagctccgt cggcagatcg tcgagcgcga gcaggtcccg gatcgcgttg cggtactcgg
     1621 tgcgcgtcag ccgccgaaac gccggcacgt cgcccggttg cggccggctc gccgcggcga
     1681 cgtcgagctg cgactcgagg aacgtcgcgg cgcgaacgta cgaagcgtgc tcgggtcgcg
     1741 gcccttcggg cggcatcgtc tcggagcgca gcttgcggac gatcttttcc cagtgctctg
     1801 gatcggctgc gatcgcgtcg accttcgctg gctcgatgac gaggtcggcc gtgaagtcgg
     1861 ctgcgttatg gcaatcggtg cagtagcggc cgagcaggtc gcgcgcggcg gccgcgtcgt
     1921 cgtgctggta gccgagccac cacgccgcgc cgagcgccga agctgtgagg actgcgagac
     1981 cgacgacgta gcggcgcaat aggcacccga cgaaaacggt tcgcctcagg ataggtcgtt
     2041 ctcgcgacgc ttgccacggg ggcgcacatg gccgcggccg cgggccgggc gtatatttct
     2101 ggagacggtt acggcagggg gcgcacgcat ggttggggct cgcatcgttc tcgcattgct
     2161 ggttgcgggc agtctgccga gcgtggccag cgcgaccccg ctgatcgatg ccgtgaagcg
     2221 ccacgacggc gtcgccgtca atgcactgct cgatcgcggc gctgacgtc
//
LOCUS       DATLEE010000059         4182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6742673, whole genome shotgun sequence.
ACCESSION   DATLEE010000059 DATLEE010000000
VERSION     DATLEE010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6742673"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VM692_01250"
     CDS             <1..462
                     /locus_tag="VM692_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HUQ50814.1"
                     /translation="GYARELTAEEKAKQQAILDRHIAKADAVITTAAIPGRTAPRIIS
                     KSAVEAMGSGAVIVDLAAESGGNCELTVAGETVVTPNGVKILGPRKLPSDLARHASQM
                     YARNLLNFLSPAIKKGELAIDWQDEVFAQAVLTHDKTVRHAGTAKLLEAAR"
     gene            459..767
                     /locus_tag="VM692_01255"
     CDS             459..767
                     /locus_tag="VM692_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HUQ50815.1"
                     /translation="MIDGFIALYIFMLAAFTGYEIIGRVPVILHTPLMSGSNFVHGIV
                     LVGAMVAMGRADSTLELTIAFIGVVLAAGNAVGGYAVTERMLEMFKSSKGAAKKGSHQ
                     "
     gene            764..2146
                     /locus_tag="VM692_01260"
     CDS             764..2146
                     /locus_tag="VM692_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HUQ50816.1"
                     /translation="MNTLGAATAIQASYFVAAALFIFGLKRMSSPVTARSGIVWAGVG
                     MVVATLITFLTPGMGNFGLMTVAIAVGGIAAWVTGRRVAMTDMPQMIALYNGMGGGAA
                     GAIAAVELFAGEVHSVAALILGVLGGIIGAVSFSGSLIAFGKLQGLIKRSFRFRGQNL
                     VNIALLVATLATGAVIVAQGGGLHPVTALFLFALVLGVTMTLPIGGADMPVVISLYNA
                     LTGLAVAFEGFVLNNAAMIIAGTVVGAAGTLLTQLMAKAMNRSLANVLFSGFGDTGGV
                     AATGGPAGTLKAIETADAAVMMGFSEKVIVVPGYGMAVAQAQHKVWELCQALIARGVT
                     VKFAIHPVAGRMPGHMNVLLAEAGVPYDIIYDEGEINAEFETADVALIIGANDVVNPV
                     ARTDKSSPIYGMPILNADKAKNVIVIKRGQGAGFSGIENALFYLDHTRMLYGDGQAKA
                     AELVQAVKAL"
     gene            complement(2168..2635)
                     /locus_tag="VM692_01265"
     CDS             complement(2168..2635)
                     /locus_tag="VM692_01265"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HUQ50817.1"
                     /translation="MKANKVVATCVALLALLAGAANAQGVRWNELSEAQRQLLAPHEQ
                     NWAQLEPQRQREIARGAERWLEMNRRDRAQALDRFQIWRGMTDEQRAAARQRYQEFRR
                     LPPTDRQRLIDVYRRYRMMPLDRRLELRQRFRELSPEQRQILRDRRMRPPPNR"
     gene            complement(2622..2867)
                     /locus_tag="VM692_01270"
     CDS             complement(2622..2867)
                     /locus_tag="VM692_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50818.1"
                     /translation="MRTASSTLLALGCWWLAAAQAQETEQPPPAPDLDFLEYLGTWAE
                     EDDEWLAIEEWQKDQAPDRDSDTQGSGTEKDDDDESE"
     gene            complement(2867..3250)
                     /locus_tag="VM692_01275"
     CDS             complement(2867..3250)
                     /locus_tag="VM692_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50819.1"
                     /translation="MSDELDSGDFERRTKRVFDDSVAALDAATRSRLTQARHRALEER
                     HGARETGWRWSLIPLGTLAATALVAWFTVGYVPIASDNVRSAALGDLEILLGEEDLEM
                     LDEEIEFYGWLEEQPEFVGAGDSVG"
     gene            complement(3243..3842)
                     /locus_tag="VM692_01280"
     CDS             complement(3243..3842)
                     /locus_tag="VM692_01280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUQ50820.1"
                     /translation="MSIARHEASLDRSQALDSGAEIEAFLSEVEGRAYRIALVSVRDR
                     GEALDIVQDAMIRLVQRYARRPKAEWPPLFYRILQNRIRDIQRRRAVRSRFVSFFGGT
                     ASEEEHDPLAEAPAPPGDDPLERAKAGDAMAALEKALLTLPARQREAFTLRNFEGLDV
                     AQTAVAMECTEGSVKTHYSRAVHRLRELMAPHGEEHWDE"
     gene            4013..>4182
                     /locus_tag="VM692_01285"
     CDS             4013..>4182
                     /locus_tag="VM692_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017409698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50821.1"
                     /translation="MSTSPRLIRVAVPVPLADAFDYLAPAGPLPAAGCRVRVPFGRGE
                     RLGIVVDHPSAT"
BASE COUNT          679 a         1446 c         1331 g          726 t
ORIGIN      
        1 ggctacgcgc gcgagctgac ggccgaagaa aaggcgaaac aacaagcgat cctcgaccgc
       61 cacatcgcga aggccgatgc cgtcatcacg acggccgcga tcccgggccg cacggcgccg
      121 cgcatcatct cgaagagtgc cgtcgaggcc atgggctccg gcgccgtcat cgtcgacctg
      181 gccgccgaga gcggcggcaa ttgcgagctc accgtcgccg gcgagaccgt cgtgacaccg
      241 aacggcgtca agatcctagg gccgcgcaaa ttaccatcgg atctcgcgcg gcatgccagc
      301 cagatgtacg cgcgcaatct gctgaacttt ctgtcgcccg ccatcaagaa gggcgagctc
      361 gcgatcgatt ggcaggacga ggtattcgct caggcggtgc taacgcacga caagaccgtg
      421 cgccacgcgg gcaccgcgaa gttgctggag gcggctcgat gatcgacgga ttcatagcgc
      481 tctacatctt catgctcgcc gccttcacgg gctacgaaat catcgggcgc gtgccggtca
      541 tcctgcacac gccgctgatg tcgggctcga acttcgttca cggcatcgtg ctcgtcggcg
      601 cgatggtcgc gatgggccgc gccgactcga cgctcgagct gacgatcgcg ttcatcggcg
      661 tcgtgctcgc agccggcaac gccgtcggcg gttacgccgt gacggaacgc atgctcgaga
      721 tgttcaagag cagcaagggc gcggccaaga agggctcgca ccaatgaata ctctcggcgc
      781 cgcgacggcg attcaggcca gctacttcgt agcagctgcg ctcttcatct tcggcttgaa
      841 acggatgagc tcgccggtga cggcgcgcag cggcatcgtc tgggccggcg tcggcatggt
      901 cgtcgccacg ctgatcacgt ttctcacgcc cggcatgggc aacttcggtc tcatgaccgt
      961 tgcgatcgcc gtcggcggca tcgcggcctg ggtcaccggc cgccgagtcg cgatgaccga
     1021 catgccgcag atgatcgcgc tctacaacgg catgggcggc ggcgcggccg gcgccatcgc
     1081 ggccgtcgag ctgttcgccg gagaggtgca cagcgtcgcg gccctgatcc tgggagtgct
     1141 cggcgggatc atcggggccg tatcgttctc gggcagcctg atcgcgttcg gcaagctgca
     1201 aggactcatc aagcgcagct tccgcttccg cggtcagaac ttggtcaaca tcgcgttgct
     1261 cgtcgcgacg ctggccaccg gcgccgtgat cgtcgcgcaa ggcggcggcc tgcatcccgt
     1321 cacggcgttg ttcctgtttg cgctggtgct cggagtcacg atgacgctgc cgatcggcgg
     1381 cgccgacatg cccgtcgtga tctcgctgta caacgcgctg acgggcctcg cggttgcctt
     1441 cgaaggcttc gtgctgaaca acgccgcgat gatcatcgcc ggcacggtcg tcggcgcggc
     1501 cggcacgctg ctgacgcagc tgatggcgaa ggccatgaac cgctcgctcg cgaacgtgct
     1561 gttcagcggc ttcggcgaca ccggcggcgt cgcggcgacg ggcggcccgg ccggcacgct
     1621 gaaagcgatc gagaccgccg atgcggccgt catgatgggc ttctcggaga aagtcatcgt
     1681 cgtgccgggc tacggcatgg cggtcgcgca ggcgcagcac aaagtctggg agctctgcca
     1741 ggcgctgatc gcgcgcggcg tgacggtgaa attcgcaatc catcccgtcg cggggcgcat
     1801 gccgggtcac atgaacgtgc tgctggcgga agccggcgtc ccctacgaca tcatctacga
     1861 cgagggtgag atcaacgccg agttcgagac agcggacgtc gcgctgatca tcggcgccaa
     1921 cgacgtcgtg aaccccgttg cccgcaccga caaatcgagc ccgatttacg gcatgccgat
     1981 cctgaacgcc gacaaggcca agaacgtcat cgtcatcaag cgcggccaag gcgcgggctt
     2041 ttcgggcatc gagaacgcgc tgttctatct cgaccacacg cgcatgctgt acggcgacgg
     2101 ccaggcgaag gccgccgagc tcgtgcaggc ggtcaaagca ctctagcctg ccgagtcgcg
     2161 ttcggcgcta gcggttcggg ggtggacgca tccggcgatc gcgcaggatc tgccgctgct
     2221 ccggcgacag ctcgcgaaac cgttgccgca gctcgagacg tcggtcgagc ggcatcatgc
     2281 gatagcgccg atagacgtcg atgagccgtt gccgatcggt cggcggcagc cgccggaact
     2341 cctgataacg ctgtcttgcc gccgcacgct gctcgtccgt catgccgcgc caaatctgga
     2401 agcggtccaa agcttgcgcg cgatcgcgtc gattcatctc gagccaacgc tccgcgccgc
     2461 gagcgatctc tcgctgacgc tgcggctcga gctgcgccca attctgctcg tgcggagcca
     2521 gcaactgccg ctgcgcctcg ctcaactcgt tccaccggac gccctgcgcg ttcgctgcgc
     2581 cggctagcag cgcgagcaat gcaacgcagg ttgcaacgac tttattcgct ttcatcgtcg
     2641 tcatccttct cagtcccgga tccttgtgtg tccgagtcgc gatctggggc ttggtccttc
     2701 tgccactctt cgatcgcgag ccactcgtcg tcctcttctg cccaggtgcc gagatactcg
     2761 agaaagtcca agtccggcgc gggtggcggc tgctcggttt cctgcgcttg cgcggccgcc
     2821 agccaccaac agccgagcgc caataacgtg gagcttgctg tgcgcatcaa ccgacgctgt
     2881 cgcccgcacc gacgaactcc ggctgttcct cgagccaacc gtagaactcg atttcctcgt
     2941 cgagcatctc gagatcctct tcgccgagca aaatctcgag atcgccgagc gccgccgatc
     3001 gcacgttgtc gctcgcaatc ggtacatagc cgacggtgaa ccacgcgacg agcgccgtgg
     3061 cggccaaggt tccgagcgga atcagcgacc accgccagcc cgtctcgcgt gcgccgtggc
     3121 gctcctcgag tgctcgatgg cgcgcctgcg tgagccgcga ccgcgtcgcg gcatcgaggg
     3181 cggccacgct gtcgtcgaat acgcgcttgg tgcggcgctc gaaatcgccg ctgtccagtt
     3241 cgtcactcat cccagtgctc ctcgccgtgc ggcgccatga gctcgcgcag ccgatgcacg
     3301 gcccgcgagt aatgcgtctt cacactgcct tccgtgcatt ccatcgcaac cgctgtctgc
     3361 gccacatcga gcccttcgaa gttgcgcagc gtgaacgcct cgcgttgccg cgccggcaac
     3421 gtcaagagcg ccttctcgag cgccgccatc gcgtcgccgg ctttggcgcg ttcgagcggg
     3481 tcatcgcccg gcggtgccgg cgcctccgcg agcggatcgt gctcttcctc ggacgccgtg
     3541 ccgccgaaga aactcacgaa ccgcgaccgc accgcgcggc gtcgctgaat gtctcgaatc
     3601 cgattctgca gaatccgata aaacagcggc ggccattcgg ccttcggccg gcgggcatag
     3661 cgttgcacga gccggatcat cgcgtcctgc acgatgtcga gcgcctcgcc gcgatcgcga
     3721 acgctcacga gcgcgatgcg atacgcacgc ccctcgacct cgctcaggaa cgcctcgatc
     3781 tccgctcccg aatccagagc ttggctcctg tcgaggctcg cttcatgccg agcgatgctc
     3841 aatgctaacc ccactctcgc cactccgtgg ccgacgtccg gtgctcgagt tgccactatt
     3901 cggccgttcg atgcaggcca taacggcccg gccgcgtcgt gggttgacag ccggcgcagc
     3961 cgcttaagtc attgaccgcg tgagcgtttc tgttcacata atcgcgcccc cgatgagcac
     4021 gagcccacgc ttgatcagag tcgccgtgcc ggtgccgctc gccgacgcgt tcgactatct
     4081 cgccccggcc ggaccgctgc cggcggccgg ctgtcgcgtg cgcgtgccgt tcggccgcgg
     4141 cgagcgactc ggcatcgtgg tcgaccatcc ttcggcgacc ga
//
LOCUS       DATLEE010000060         2135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2310122, whole genome shotgun sequence.
ACCESSION   DATLEE010000060 DATLEE010000000
VERSION     DATLEE010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310122"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1071)
                     /locus_tag="VM692_01290"
     CDS             complement(139..1071)
                     /locus_tag="VM692_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUQ50822.1"
                     /translation="MTDSKRLLVVVDPTASFHPAIERAAWLAHHTQARVELFISDYSA
                     NLADGRSANAAGSRTAVLERHRKRLELLAEPLRKAGIMVEIDARWDYPLHDSIVRKAI
                     DGAADVVIKDTHYHSALQRSIFSNTDWSLIRACAATLWLVKPRPPGQRPCLIAAVDPL
                     HERDKPADLDNKILATATQLAVALGGEVHAFHAFDVSAAIAVATDSMTMPIALPIEDF
                     TEAIRAEHGDAVAELCAAHGIASERTHVHQGVTRDLLMTLTEQLRADAVIMGAISRSG
                     LKGLFLGNTAEDVLDRLHCDLIIVKPEAFKAALP"
     gene            1207..>2135
                     /locus_tag="VM692_01295"
     CDS             1207..>2135
                     /locus_tag="VM692_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUQ50823.1"
                     /translation="MIVAQFQALLDAAVDAIIIIDERGQILTFNRAAERMFGHAAAEV
                     VGKSVTMLMNEPHRTQHEQYVSRYVATGDAHIIGKGREIEARRANGEVFPASLAVGEA
                     VDAHGRRFVGIVRDLSTQRAAEQRTRALELRLAHVARFNLMGEMAAGIAHEINQPLSA
                     IATYAQAAKRVMQGTEPDMAMVMDACGKIDEQARRAGQVIDNLRRLIKKQEIETESLD
                     VNRVVGDVLSLIEADAHSEGIPVHVLAADGLPSVRADAVQLQQVLLNLTRNSVDAMRD
                     GLGKERGIIIATQRGERGGVRITVTDHGHGVSR"
BASE COUNT          371 a          677 c          731 g          356 t
ORIGIN      
        1 cggcttcgag cgccgtaatg cgctcctgcg ccgtcggcgc tgcgcgcagc ggctcctgcg
       61 cagccgcgga cgccatggcg gccagagccg ccagcgtcgt cgcgtaacga acagtcgtat
      121 acattgcaac ctccttggct atggcagcgc ggctttgaac gcttccggtt tgacgatgat
      181 gagatcgcag tgcagccggt cgaggacgtc ttcggcggtg ttgccgagga acagcccctt
      241 caagccgctg cgcgagatcg cgcccatgat gacggcgtcg gcgcgcaact gctccgtcaa
      301 cgtcatcagc aagtcgcgtg tgacgccttg gtggacatga gtgcgctcgc tcgcgatgcc
      361 gtgcgccgca cacagctcgg cgacggcgtc gccatgctcg gcgcgaatgg cttccgtgaa
      421 gtcctcgatc ggcaatgcaa tcggcatcgt catcgagtcg gtcgcgacgg cgatcgcggc
      481 ggagacgtcg aacgcatgga acgcgtgcac ctcgccgccg agcgcaacgg ccagttgcgt
      541 cgcggtcgcg aggatcttgt tgtcgaggtc ggcaggtttg tcgcgctcgt gcagcgggtc
      601 gaccgcggcg atgagacagg ggcgctgccc cggtgggcgc ggcttgacga gccaaagagt
      661 cgcggcgcag gcacggatca ggctccagtc cgtgttcgag aagatcgagc gctgcaatgc
      721 ggagtggtag tgcgtgtcct tgatgacgac gtccgccgcg ccgtctatgg ccttacgcac
      781 gatgctgtcg tgcagcgggt aatcccagcg cgcgtcgatc tcgaccatga tgcccgcttt
      841 acgcagcggc tcggcgagta gctcgagccg cttgcgatgc cgctcgagca cggcagtgcg
      901 cgacccggcg gcgttggccg agcgcccatc ggcgaggttg gccgaataat cgctgatgaa
      961 cagctcgacc cgcgcttgcg tatggtgcgc gagccaggcg gcgcgctcga tcgcaggatg
     1021 gaagctggcg gtcgggtcga cgacgacaag taagcgtttg gagtcggtca tatgccacct
     1081 gccaaggttg ccgcacgcta cctttatact gagtgcgccg acgcggcgga gttcgctgaa
     1141 acgcctagcg ccgtgcggca actacgtatg gttcgacctt aggtttcggc cgcgaaatac
     1201 gctgcaatga tcgttgccca gtttcaagct ctgctcgacg cggcggtcga cgcgatcatc
     1261 atcatcgacg aacgcggtca gattttgacg ttcaaccgcg ccgccgagcg gatgttcggc
     1321 cacgcggcgg ctgaggtcgt cggcaagagc gtgacgatgc tgatgaacga gccgcaccgc
     1381 acgcagcacg agcagtacgt ctcgcgctac gtcgcgacgg gcgacgccca catcatcggc
     1441 aagggacgcg aaatcgaggc tcgacgggcc aacggcgagg tctttcccgc gtcgctggcg
     1501 gtcggcgagg ccgtcgatgc gcacggacga cgcttcgtcg gcatcgtccg cgacctctcg
     1561 acgcagcgag cggcggaaca gcgcacgcgc gcattggagc tgcggctcgc gcacgtcgcg
     1621 cgcttcaact tgatgggcga gatggccgcc ggcatcgctc acgagatcaa tcagccgctc
     1681 agcgcgatcg cgacctacgc ccaggctgcg aagcgcgtca tgcagggcac ggagcccgac
     1741 atggcgatgg tcatggacgc ctgcggcaag atcgacgagc aagcgcgtcg cgcggggcag
     1801 gtcatcgaca atctgcgccg gttaatcaag aagcaagaga tcgagacgga gtcgctcgac
     1861 gtcaatcgtg tcgtcggcga cgtgctgagc ctcatcgaag ccgacgcgca ttccgaaggc
     1921 atccccgtgc acgtgctcgc agctgatggg ctgcccagcg tgcgtgccga cgccgtgcag
     1981 ctacagcaag tcttgctgaa tctgacccgc aactcggtcg acgccatgcg cgacggcctc
     2041 ggcaaggagc gcggcatcat catcgcgacg cagcgcggcg agcgcggcgg cgttcggatc
     2101 acggtcaccg atcatggtca tggtgtgtcg cgcca
//
LOCUS       DATLEE010000061         3031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3801506, whole genome shotgun sequence.
ACCESSION   DATLEE010000061 DATLEE010000000
VERSION     DATLEE010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3801506"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1738)
                     /locus_tag="VM692_01300"
     CDS             complement(<1..1738)
                     /locus_tag="VM692_01300"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HUQ50824.1"
                     /translation="MAQFARPLLLSLAVLGGCAEPPPPAQTPAPAPPAASAASIDGFF
                     QRFTDDWVRRNPNLAISTRYFEGEEQDRLSRQITPVSKAYELETIAIARRGLTELATF
                     ERGTLTLDQQLSADIMRWQLQNAVDGEPFLDYSFPLEQMNGVNVLLPNQLTVVQPVAT
                     ARDAENYVARLGQFDERMAEAVEESARQAEAGVLPPSFIFTATIAQMQRFIAPPAAEN
                     PLVTTLREKMADVAELSGARRDELAAAAAAVVADEVYPAWRTAIAQLEAQMPRATADA
                     GLGRFEGGAALYAQRLHNFTTTTLTAEEIHAIGLAEVARIEAEMERLFRSLGLAEGTI
                     TEREEQLSARLSYPNTDEGRREIMSDIDGILSDALARTADSFDVRPATPIVAQPYPQF
                     RWDNAAASYTPPPSDRSRPGIFQMPLRPSQLTRFALRSLVYHETVPGHHFQIALAVEN
                     ERLPAFRRVRALGGLSASSEGWALYAERFAAEDGWYEGDVAGQLGQLSDALFRARRLV
                     ADTGLHAMGWTREQAIDLGLEASEVERYVVYPGQACSYMIGQLRILELRERAKAALGE
                     KFSIKEFHNVLLR"
     gene            complement(1762..>3031)
                     /locus_tag="VM692_01305"
     CDS             complement(1762..>3031)
                     /locus_tag="VM692_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUQ50825.1"
                     /translation="GLVSCFALAALWPLAAGSQPNDGARGAVPESVQVLDEVIERLTD
                     APLYRDNDVELLLDGPRTHDAMRDAIASAEHEVHLETYIFAADEVGTEFAEALAAKAR
                     EGVTVRVIYDAIGSRGGEEGFWTELTNAGVLVREFNPPDPVQDQNPFDIDTRDHRKLL
                     IVDGRVAFTGGINIDRNYVHASDVVGGRSASSGWRDTHIRIEGPAVAAFQDLFVGLWE
                     KLDEPLAKPPYAPPARRLDQGNTRVRVLAGVGGNDEVSPIWVAYEAAAKVAAKRIWIT
                     QSYFAPDDELLAAITEAAGRGVDVRILVAGFSDSDLLLNASRAYYADLLEAGVKVFES
                     QENILHAKTMVVDGYWSTVGSSNLDYLSFLHNHEANAIVLGAPFAAQLEDVFLADLNN
                     AKPIDAKQWRQRSLWQRTKELGSYFIRYRL"
BASE COUNT          462 a         1029 c         1034 g          506 t
ORIGIN      
        1 cgcgcagcag gacgttgtgg aattccttga tcgagaattt ctccccgagc gcggccttgg
       61 cccgctcgcg cagttcgaga atccgaagct gtccgatcat gtacgagcaa gcctgccccg
      121 ggtacacgac gtagcgctcg acctcgctgg cttctagccc caagtcgatc gcttgctcgc
      181 gcgtccaacc catcgcgtgt aggccggtgt cggcgacgag gcggcgcgcg cggaacagcg
      241 cgtcgctgag ctgcccgagc tgccccgcga cgtcgccctc gtaccaaccg tcctcggccg
      301 cgaagcgctc ggcgtagagc gcccagccct cggagctcgc cgacagccca ccgagcgcac
      361 gcacgcggcg aaacgccggc agccgctcgt tctctacggc cagcgcgatc tggaagtgat
      421 ggccgggtac cgtctcgtga taaacgaggc tgcgcagcgc gaagcgtgtc agctggctcg
      481 gccgcagcgg catctggaag atgccgggcc gcgaccggtc gctcggcggc ggcgtgtagc
      541 tcgccgcggc gttatcccag cgaaattgcg gatacggctg cgcgacgatc ggcgtcgcgg
      601 ggcgcacgtc gaacgagtcg gccgtgcgcg ccaacgcgtc gctgagaatg ccgtcgatat
      661 cgctcatgat ctcgcggcgg ccctcgtcgg tgttcggata gctcaagcgc gcgctcaatt
      721 gctcctctcg ctctgtgatc gtgccctcgg cgagccccag cgagcggaac aaccgttcca
      781 tctcggcttc gatgcgcgcg acttctgcga ggccgatcgc gtgaatttcc tcggccgtca
      841 acgtcgtcgt cgtgaagttg tgcaggcgct gcgcgtagag cgccgcgccg ccttcgaagc
      901 gtccgagccc cgcgtccgcg gtcgcacgcg gcatctgtgc ttcgagctgc gcgatcgccg
      961 tgcgccacgc ggggtagacc tcatcggcga ccacggccgc ggccgccgca gcaagctcgt
     1021 cgcggcgcgc ccccgagagt tccgcgacat cggccatctt ctcgcgcaag gtcgtgacga
     1081 gcggattctc cgccgcaggc ggcgcgatga aacgctgcat ctgcgcgatc gtcgccgtga
     1141 agatgaagct cggcggcagc actccggcct cggcttgccg cgcgctttcc tcgacggcct
     1201 cggccatgcg ctcgtcgaac tggccgaggc gcgcgacgta gttctcggcg tcgcgcgccg
     1261 tcgcgacggg ctgcacgacc gtgagctgat tcggcagcag aacgttgacg ccgttcatct
     1321 gctccagcgg aaagctgtag tcgaggaacg gctcgccgtc gacggcgttc tgcaactgcc
     1381 agcgcatgat gtcggccgag agctgctgat ccaaagtcag cgtgccgcgc tcgaaggttg
     1441 cgagctccgt cagcccgcgg cgagcgatcg cgatcgtctc gagctcgtag gctttgctga
     1501 ccggcgtgat ttgccggctc agccgatctt gctcctcgcc ctcgaagtaa cgcgtgctga
     1561 tcgcgagatt cggattgcgg cgcacccagt cgtccgtgaa gcgctggaag aagccgtcga
     1621 tcgaagccgc cgatgcggcg ggcggcgccg gcgccggcgt ctgggccgga ggcggcggct
     1681 cggcgcagcc gccgagcacc gcgagcgaca gcagcagcgg gcgtgcgaac tgggccatga
     1741 gcttcgacct ccgagcgccc gttacagtcg gtaacgaatg aagtagctgc cgagctcctt
     1801 ggtgcgctgc cacagcgagc gctgtcgcca ctgcttggcg tcgatcggtt tggcgttgtt
     1861 cagatcggca agaaacacgt cctcgagctg ggcggcgaac ggcgcgccga gcacgatcgc
     1921 gttcgcctcg tgattgtgca ggaagctcag gtaatccaag ttgctgctgc cgaccgtgga
     1981 ccagtacccg tccacgacca tggtcttggc atgcaggatg ttctcttggc tctcgaagac
     2041 cttgacgccg gcttcgagca ggtcggcgta ataggcgcgc gacgcgttca acaacaaatc
     2101 cgagtccgag aaaccggcga cgagtatgcg gacgtcgacg ccgcgcccgg ccgcctcggt
     2161 gatcgcggcc agcagctcgt cgtcgggagc gaagtacgac tgagtgatcc agatgcgctt
     2221 ggccgccact ttggccgcgg cttcgtaagc cacccagatc ggcgagacct cgtcgttgcc
     2281 gccgacgccc gcgagcacgc gcacgcgggt gttgccttgg tcgagccggc gcgcgggcgg
     2341 tgcgtacggc ggtttcgcga gcggctcgtc gagcttctcc cataggccca cgaacaaatc
     2401 ctggaatgcg gcgacggccg ggccctcgat gcggatgtgc gtatcgcgcc aacccgacga
     2461 cgcgctgcgt ccgccgacga cgtccgacgc gtgcacatag ttgcgatcga tgttgatgcc
     2521 gcccgtgaag gcgacgcggc cgtcgacgat cagcagcttg cgatggtcgc gcgtgtcgat
     2581 gtcgaacggg ttctggtcct gcacggggtc cggcgggttg aactcgcgga cgagcacgcc
     2641 ggcgttcgtc agctcggtcc agaagccctc ttcgccgccg cggctgccga tggcgtcgta
     2701 gatcacgcgc accgtcaccc cttcgcgcgc tttggccgcg agcgcctcgg cgaattccgt
     2761 gccgacttcg tcggcggcga agatgtaggt ctcgagatgg acctcgtgct cggccgacgc
     2821 gatcgcgtcg cgcatcgcgt cgtgcgtccg cgggccgtcg agcagcagct cgacgtcatt
     2881 atcccgatag agcggcgcgt cggtcaggcg ctcgatgacc tcgtcgagca cttggaccga
     2941 ctccggcacg gcgccgcgcg cgccgtcgtt cggctgcgag ccggcggcga gcggccatag
     3001 cgctgccagc gcgaagcacg aaacgaggcc g
//
LOCUS       DATLEE010000062         5781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9014894, whole genome shotgun sequence.
ACCESSION   DATLEE010000062 DATLEE010000000
VERSION     DATLEE010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9014894"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..994
                     /gene="ntrB"
                     /locus_tag="VM692_01310"
     CDS             167..994
                     /gene="ntrB"
                     /locus_tag="VM692_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HUQ50826.1"
                     /translation="MHAPAKRARSARVWAATQRHAVGVLPPLIAVAAIVGLWELLCAA
                     PDASLPPPSRVVAETWELIVDPFYDRGGIDKGLFWHIYASIKRVALGYLLASIAGIGL
                     GVLVGSSKLATRALDPIFQVLRTIPPLAWLPLSLAAFRDAQPSAIFVIFITAVWPIII
                     NTAVGIRNIPQDYQNVAKVLRLNRGEYFTAIMLPAAAPFIFTGLRIGVGLSWLAIVAG
                     EMLIGGVGIGFFIWDSWNSSRISDIILALLYVGLVGLLLDRAIAILGRVVTRGTAST"
     gene            1005..1808
                     /locus_tag="VM692_01315"
     CDS             1005..1808
                     /locus_tag="VM692_01315"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006699924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ50827.1"
                     /translation="MEGNDRYLQIEHVFKSFGGAAGTEVLRDVSLTVRKGEFVSIIGH
                     SGCGKSTLLNIVAGLTPTTAGNVFVDNREVVEPGPDRAVVFQNHSLLPWLSVYQNVRL
                     AVDKIFGATMSKAERDEWTLRNLELVDMAPARGKKPGEISGGMKQRVGSARALAMKPK
                     ILLLDEPFGALDALTRAHLQDRVMQIHMELGSTVMMITHDVEEAVLLCDRIVMLTNGP
                     SATIGAVCEVPLVRPRQRLELIGDPTYVGCRAAVLEFLHRRHRFVEAAA"
     gene            1827..4283
                     /gene="nirB"
                     /locus_tag="VM692_01320"
     CDS             1827..4283
                     /gene="nirB"
                     /locus_tag="VM692_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018941272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HUQ50828.1"
                     /translation="MSKRKLVLIGNGMAGMRAVEELLKLAPDMYDITVFGSEPHGNYN
                     RILLSPVLAAEKTVGDIMLNTREWYAENGITLHAGEEVVRVDRARRRVVAKSGLEVPY
                     DRVILATGSVPIVLPLPGKELEGVVSYRDIADVDRMIDASRQFKHAVVIGGGLLGLEA
                     AYGLQKRGMDVTVVHLGATLMERQLDASAGLLLKRSLEERGLKFVMPAQTAAILGQQR
                     VRGVRFADGSELPADLVVMAVGIRPNAALAKASGIACERGVLVNDTMQTYDPRIYAVG
                     ECVQHRGSCYGLVAPLFEQAKVVANHLAEFGIGRYPGSQTSTKLKVTGIDLFSAGDFT
                     GDADTEELVFKDAARGVYKKLVVKGNKIQGAVLYGDTVDGAWYFQLMRDGTAISDFRE
                     NILFGQAHIGDSGTAGADAVTLLTDAAEICGCNGVCKGDIVKAITRKNLFTLDEVRAH
                     TKASSSCGSCTGLVEQLLANTLGGDYSEAPAKKPMCPCTIYTHEEVRAAIARERLKAI
                     PAAMQFLEWQTPDGCHKCRPALNFYLLCAWLGEYQDDKQSRFINERAHGNIQRDGTYS
                     VVPRMWGGVTSAKELRAIADVAEKYAVPTVKVTGGQRIDLLGVKKEDLPSVWRDLNAA
                     GFVSGHAYGKALRTVKTCVGKQWCRFGTQDSTGLGIHLERVTWGSWHPHKVKLAVSGC
                     PRNCAEATIKDLGVVCVDSGYELHVGGNGGIKIRGTDFLCKVATEGEVVEYCCAYLQL
                     YREEARYLERTAPWIERVGLTHVRERIVDDAKSRQALHARFLESQRYSQTDPWAERAA
                     GKQADEFSALHAEPLAELAS"
     gene            4280..4612
                     /gene="nirD"
                     /locus_tag="VM692_01325"
     CDS             4280..4612
                     /gene="nirD"
                     /locus_tag="VM692_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HUQ50829.1"
                     /translation="MSWVDLGPVTALPERGARCVRVGAMAIAVFRTSTGQVFALRDQC
                     PHRGGPLSQGIVHGTRVTCPLHDWVIDLATGKATGADEGSTPTFNVRVLDGRIALELP
                     AELLANAS"
     gene            4609..>5781
                     /locus_tag="VM692_01330"
     CDS             4609..>5781
                     /locus_tag="VM692_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015887235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUQ50830.1"
                     /translation="MTQAVRTTCPYCGVGCGLLATRSGEATYEIRGDPEHPANLGRVC
                     SKGAALGETLDLDGRLLHPEIGGERASWDEALDAAAAGFARTIAAHGPDSVAFYVSGQ
                     LLTEDYYVANKLMKGFIGSANIDTNSRLCMASAVAGYKRAFGADAVPNDYTDLEQADL
                     VLLVGSNLAWCHPVLFQRLSAAKAANPRLRVVLIDPRRTQTAEIADQFLQLRPGTDVL
                     LFNGLLNYLRKEDALDLEFLDAHTQGFGAALRSAKDSAASIPAVALGCGLAEDDVLEL
                     YRAFARTPRTVTAFSQGVNQSTSGTDKVNAIVNVHLATGRIGKPGMGPFSLTGQPNAM
                     GGREVGGLANQLAAHMDFAPGDVARVARFWNAPRVASKPGLKAVDLFGAVERGAVKA"
BASE COUNT          958 a         1896 c         1959 g          968 t
ORIGIN      
        1 gatcccgcga acccgtcggc gtacctcgcc agcctcgcga tcaagcgcgc gagctgatgc
       61 aatgagctcc actgcacgat cgctgccgca cacgcacgag agcaaggtga tcccgttgaa
      121 cgcggaggcc gcgccgctcg gcgcgagccc tgcgcgcgtg ccgggcatgc acgcgcccgc
      181 gaagcgggcc cgctccgcgc gcgtgtgggc ggccacgcag cggcacgctg tcggcgtgct
      241 gccgccattg atcgccgtgg ccgcgatcgt cgggttgtgg gagctgctgt gcgccgctcc
      301 cgacgcgagc ctaccgccgc cgtcgcgcgt ggtcgcggaa acctgggagc tcatcgtcga
      361 tccgttctac gaccgcggcg gtatcgacaa gggcctgttt tggcacatct acgcgagcat
      421 caagcgcgtc gcgctcggct acttgctggc gtcgatcgcc gggatcgggc tcggcgtgct
      481 cgtcggcagc agcaagctcg cgacgcgcgc gctcgatccg atctttcaag tcttgcgcac
      541 gattccgccg ctcgcgtggc tgccgctgtc gctggccgcg tttcgcgacg cccagccgtc
      601 ggccatcttc gtgatcttca tcaccgccgt ttggcccatc atcatcaaca cggcggtcgg
      661 catcaggaac atcccgcagg attatcagaa cgtggcgaag gtgctgcgtc tcaatcgcgg
      721 cgagtacttc acggcgatca tgctgcctgc ggccgcaccg ttcatcttca cgggcttgcg
      781 gatcggcgtc gggctctcgt ggctcgccat cgtcgccggc gagatgctga tcggcggcgt
      841 cggcatcggc ttctttatct gggattcgtg gaacagctcg cgcatcagcg acatcatcct
      901 ggcgctgctc tacgtcggcc tggtcggcct gctgctcgat cgggcaatcg cgattctcgg
      961 ccgcgttgtc acacgcggca cggcaagcac ctgaaggagc cggcatggaa ggcaacgacc
     1021 gctatctgca gatcgagcac gtcttcaaaa gcttcggcgg cgccgccggc accgaggtgc
     1081 tgcgcgacgt gtcgctgacc gtgcgcaaag gcgagttcgt gtcgatcatc ggccactcgg
     1141 gctgcggcaa gtccacgttg ctaaacatcg tcgcggggct gacgccaacg accgccggca
     1201 acgtcttcgt cgacaatcgc gaggtcgtcg agccgggccc cgaccgcgcc gtcgtattcc
     1261 agaaccactc gttgcttccg tggctcagcg tctatcagaa cgtgcgcttg gccgtcgaca
     1321 aaatattcgg cgctacgatg tcgaaggcgg agcgcgacga gtggacgcta cgcaacctcg
     1381 agctcgtcga catggcgccg gcgcgcggca agaagcccgg cgagatctcg ggcggcatga
     1441 agcagcgagt cggcagcgcg cgcgcactgg ccatgaagcc gaagattctg ctgctcgacg
     1501 agccgttcgg agcgctcgac gcgttgacgc gtgcacacct gcaagaccgc gtcatgcaga
     1561 tccacatgga gctcggcagc acggtcatga tgatcacgca cgacgtcgag gaagccgtgc
     1621 tgctctgcga ccgcatcgtc atgctgacga acgggccgtc cgcgacgatc ggcgcggtct
     1681 gcgaggtgcc gctcgtgcga ccgaggcagc gcctcgagct catcggcgat ccaacctatg
     1741 tcgggtgccg tgcggccgtg ctcgagttcc tgcacaggcg tcatcgtttc gtcgaggcgg
     1801 cggcctagag cggccatcga cagacgatga gcaagcggaa actggtcctt ataggcaacg
     1861 gcatggccgg catgcgcgcc gtcgaggagc tcctgaagct cgcgccggac atgtacgaca
     1921 tcacggtgtt cggcagcgag ccgcacggca actacaaccg gatcttgctg tcgcccgtgc
     1981 tggcggccga gaagaccgtc ggcgacatca tgctgaacac gcgcgagtgg tacgccgaga
     2041 acggcatcac tctgcacgcc ggtgaggaag tcgtgcgcgt cgatcgtgca cgccgccgcg
     2101 tcgtcgcgaa gagcgggctc gaggtgccgt acgaccgagt gattctggcg acgggcagcg
     2161 ttcccatcgt gctgccgttg cccggcaagg agctcgaggg cgtcgtcagc taccgcgaca
     2221 ttgccgacgt cgaccgcatg atcgacgcgt cgcggcaatt caagcacgcc gtcgtcatag
     2281 gcggcggact gctcgggctc gaggccgcgt acggtctcca gaagcgcggc atggacgtca
     2341 cggtcgtgca cctcggtgcg acgttgatgg agcggcagct cgatgcgtcg gccggccttt
     2401 tgctgaagcg ctcgctcgag gagcgcgggc tcaagttcgt gatgccggca cagacggccg
     2461 cgatcctcgg ccagcaacgc gtccgcggag tccgtttcgc ggacggcagc gagttgccgg
     2521 ccgatctcgt cgtcatggcc gtcggtatca ggccgaatgc cgcgctcgcg aaagccagcg
     2581 gcatcgcctg cgagcgtggc gtgctcgtca acgacacgat gcagacctac gacccgcgca
     2641 tctacgccgt tggcgaatgc gtgcagcacc gcggctcttg ttatggcctt gtcgcgccgc
     2701 tgttcgagca agcgaaagtc gtcgcgaacc atctcgcgga gttcggcatc ggccgttatc
     2761 cggggtcgca aacgtcgacg aagctgaagg tcacgggcat cgatttgttc tcggccggcg
     2821 atttcacggg cgacgcggat accgaggagc tcgtgttcaa ggacgccgcg cgaggcgtct
     2881 acaagaagct cgtcgtcaag ggcaacaaga ttcagggcgc cgtgctgtac ggcgataccg
     2941 tcgacggcgc gtggtacttc caactcatgc gcgacggcac ggcgatcagc gacttccgcg
     3001 agaacatttt gttcggccag gcgcatatcg gcgattcggg taccgccggc gccgacgccg
     3061 tgacgctgtt gaccgacgcg gccgagatct gcggctgcaa cggcgtctgc aaaggcgaca
     3121 tcgtcaaggc catcacgagg aaaaatctgt tcacgctcga cgaggtccgt gcgcacacca
     3181 aggcttcctc ctcttgcggg tcctgcacgg gcctcgtcga gcagctcctc gcgaacacgc
     3241 tgggcggcga ctattcggaa gcgccggcga agaagccgat gtgcccgtgc acgatctaca
     3301 cgcacgagga ggtgcgcgcc gcgatcgcgc gcgagcggtt gaaggcgatt ccggcggcga
     3361 tgcagttcct cgagtggcag acgccggacg gctgccacaa gtgccgtccc gccctgaatt
     3421 tctacctgct ctgcgcgtgg ctcggcgaat atcaggacga caagcagtcg cgcttcatca
     3481 acgaacgagc gcacggcaac attcagcgcg atggcacgta ttccgtcgtg ccgcgcatgt
     3541 ggggcggcgt gacgtcggcg aaggagctgc gtgcgatcgc ggacgtcgcc gagaagtacg
     3601 ccgtgccgac cgtcaaggtc acgggcgggc agcgcatcga cttgctcggc gtcaagaagg
     3661 aagacctgcc gagcgtctgg cgcgatctga acgcggccgg attcgtctcg gggcacgcct
     3721 acggcaaggc gttgcgcacc gtgaagacct gtgtcggtaa acaatggtgc cggttcggca
     3781 cacaggactc gacggggctc ggcatccacc tcgagcgcgt gacgtggggc tcgtggcatc
     3841 cgcacaaggt caagctcgcc gtgtcgggct gcccgcgcaa ctgcgccgaa gcgacgatca
     3901 aggatctggg tgtcgtctgc gtcgactcgg gctacgagct gcacgtcggc ggcaatggcg
     3961 gcatcaagat ccgcggcacg gattttctct gcaaggtcgc gaccgaaggg gaggtcgtcg
     4021 agtattgctg tgcctatctg cagctctacc gtgaggaagc ccgctatctc gagcgcaccg
     4081 cgccgtggat cgagcgcgtc ggcctcacgc acgtgcgcga gcgaatcgtc gacgacgcga
     4141 aatcgcggca agcgctccat gcgcggttct tggagtcgca gcgctactcg cagacggatc
     4201 cctgggccga gcgcgccgcc ggcaagcaag ccgacgagtt cagcgctctg catgccgagc
     4261 cgcttgccga gctcgcgtcg tgagctgggt ggacttgggg cccgtcacgg cattgcccga
     4321 gcgcggcgcg cgttgcgtgc gcgtcggtgc gatggcgatc gccgtgtttc gcacgagcac
     4381 gggtcaggtg ttcgcattgc gtgatcaatg cccgcaccgc ggcgggccgc tgtcgcaagg
     4441 catcgtgcac ggcacgcgcg tcacgtgccc gctgcacgac tgggtcatcg atctcgcgac
     4501 gggcaaggcg acgggtgccg atgaagggtc gacgccgacg ttcaacgtgc gcgtcctcga
     4561 cggccgcata gcgctcgagc tgccggcgga gctgctcgcg aacgcgtcat gacacaagcc
     4621 gttcgcacga cgtgcccgta ctgcggtgtc ggctgcgggc tcctggctac ccgctcgggc
     4681 gaggcaacct atgagattcg cggcgatccc gaacatcccg cgaacttggg gcgcgtgtgc
     4741 tcgaaaggcg ccgcgctcgg tgagacgctc gatctcgacg gccggttgct gcaccccgag
     4801 atcggcggtg agcgcgcgag ctgggacgag gcgctcgacg ccgccgccgc cggcttcgcg
     4861 cgcacgatcg cggcgcatgg accggactcc gtggcctttt acgtctcggg acagctgctg
     4921 accgaggact actacgtcgc caacaagctc atgaagggct tcatcggcag cgcgaacatc
     4981 gacacgaact cgcgcctctg catggcctcg gccgtagccg gctacaagcg cgccttcggc
     5041 gccgacgccg tcccgaacga ctacacggat ctcgagcagg ccgatctcgt cctgctcgtc
     5101 ggctcgaatc tcgcgtggtg ccaccccgtg ctgttccagc gcctcagcgc agccaaggcc
     5161 gccaacccgc gcctcagagt cgtgttgatc gacccgcggc gcacgcagac ggccgagatc
     5221 gccgaccagt tcttgcagct tcggcccgga acagacgtgt tgttgttcaa tgggttactg
     5281 aactatcttc ggaaagaaga tgcgctcgat ttggagttcc tcgacgctca tacgcagggg
     5341 ttcggggccg cgctgcggtc tgcgaaggac agcgcggcgt cgatcccggc cgtggccttg
     5401 ggctgcggtc tcgccgagga cgacgtgctc gagctttatc gcgcgtttgc gcgcacgccg
     5461 cggaccgtca cggcgttctc gcaaggcgtg aatcagtcga cgagcggcac cgacaaggtc
     5521 aacgcgatcg tcaacgtgca cctcgcaacg ggccgcatcg gcaagccagg catggggccg
     5581 ttctcgttga cgggccaacc gaacgcgatg ggcggccgcg aggtcggcgg gctcgcgaac
     5641 cagcttgcgg cgcacatgga cttcgcgccg ggcgacgtcg cgcgcgtcgc gcgcttttgg
     5701 aatgcgccgc gcgtcgcatc gaagccaggg ctcaaggccg tcgatctgtt cggcgccgtc
     5761 gagcgcggcg ccgtcaaagc g
//
LOCUS       DATLEE010000063         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9015781, whole genome shotgun sequence.
ACCESSION   DATLEE010000063 DATLEE010000000
VERSION     DATLEE010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9015781"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VM692_01335"
     CDS             complement(<1..344)
                     /locus_tag="VM692_01335"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HUQ50831.1"
                     /translation="MSASTWTCPTCATAATSQFCPTCGERSLDERELTLHALAKQIFH
                     ALTSVDGRLLRSFALLLRHPGELTVAYLQGQRKPYLGPVPLYLMANVLFFAVESLLGA
                     NVFSTSLDYHLSR"
     gene            complement(498..1538)
                     /locus_tag="VM692_01340"
     CDS             complement(498..1538)
                     /locus_tag="VM692_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HUQ50832.1"
                     /translation="MSASTSLADLRAALRAAGARPRHETLLLRAWVRGLPLDGFAQSE
                     DARLPRLLQHALPALTAQFAALVTVRSEHRGTDSSTRLLLALGDGRTIESVLLPGDGV
                     CVSTQVGCAVGCVFCMTGRDGLARHLTSGEIVAQVALARARQRIRRVVFMGMGEPAHN
                     LDNVLEAITLLGEQGDLASKNLVFSTVGDRRAFERLPLHPVKPALALSLHTTNPELRM
                     QLLPRAPRIEPAELVALADEYSRATKYPTQYQWTLLEGVNDGDDELETLATLLQGHYA
                     VVNFIPYNTIDGADFRRPSWERAAEMTRYLHQRGVLAKVRRSAGQDVDAGCGQLRARH
                     SSVASGSRQRAG"
     gene            complement(1551..2009)
                     /locus_tag="VM692_01345"
     CDS             complement(1551..2009)
                     /locus_tag="VM692_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ50833.1"
                     /translation="MVRLRCAFALLIAFAASAAQADVSLNSVRIAAKDAEALGKFYAA
                     AFGLHETNRLQTQNGPELFLNFGANAEAARANQSPAVVIMQTKEGPVNDPVMHVILTV
                     TDAAATAAAAKAAGGTVAREAAQFSNSGIFIAFVVDPEGNRIELIQPARR"
BASE COUNT          377 a          750 c          811 g          372 t
ORIGIN      
        1 cggctgagat ggtagtcgag cgacgtcgag aaaacattcg cgccgagcag cgactcgacg
       61 gcgaagaaca gcacgttcgc catgaggtag agcggcacgg ggcccaagta cggcttgcgc
      121 tgcccttgca ggtacgcgac cgtcagctcg ccggggtgcc gcagcaagag cgcgaagctc
      181 cgtagcagcc gtccgtcgac gctcgtcagg gcatggaaga tctgcttcgc gagggcgtgc
      241 agcgtgagct cgcgctcgtc gagcgaccgc tcgccgcacg tcgggcagaa ctgcgaagtg
      301 gcagccgtcg cgcaggtcgg acaggtccaa gtgctcgcgc tcattgcgct tgcattcttg
      361 cacagccgct gcggccgcgc gccgccgcgc tgcgacaatt cgtcgcaggt cgcgctggct
      421 gcgttgctcg ggagctgcct gcgggttcag ccgctcactc gacgcagcgt ggtcgggagc
      481 tcggcgtcgg tttcggatca gccggcccgc tggcgcgagc cgctcgccac gctcgagtgc
      541 cgcgcgcgca gttggccgca gcctgcgtcg acgtcctgcc cggccgaccg tcgcaccttc
      601 gcgagcacgc cgcgttggtg caagtagcgc gtcatttcgg cggcgcgctc ccacgacggc
      661 cggcggaagt ctgcgccgtc gatcgtgttg tacgggatga aattcacgac ggcgtagtga
      721 ccttgcagca gcgtcgcgag cgtttcgagc tcgtcgtcgc cgtcgttgac gccttcgagc
      781 aacgtccatt ggtattgcgt cggatacttc gtcgcgcgcg aatactcgtc ggccagcgcg
      841 acgagctcgg ccggttcgat gcgcggtgct cgcggcagga gctgcatgcg cagctcgggg
      901 ttcgtcgtgt gcagcgacag cgcgagcgcc ggcttgacgg ggtggagggg caaacgctcg
      961 aacgcacgcc ggtcgccgac cgtcgagaag acgaggttct tgctggccag atcgccttgc
     1021 tccccaagca acgtgatcgc ttcgagcacg ttgtcgaggt tgtgtgcggg ctcgcccatg
     1081 cccatgaaca cgacgcgccg gattcgctgc cgtgcgcgcg ccagcgccac ctgtgcgacg
     1141 atttcgccgc tcgtcaggtg acgcgcgagg ccgtcacggc ccgtcatgca gaacacgcag
     1201 ccgacggcac agccgacttg cgtcgacacg cagacgccgt cgccgggcag cagcacgctt
     1261 tcgatcgttc ggccgtcgcc gagagcgagc agcaaccgcg tcgaagagtc cgtgccgcgg
     1321 tgctcggagc gcaccgtgac gagcgcggcg aactgggccg tcagtgcggg aagcgcgtgc
     1381 tgcagcaggc gcggcagcct tgcgtcctcg ctttgcgcga acccgtcgag cggcaagcct
     1441 cgcacccagg cccgcaagag caacgtctcg tgccgcggcc gcgcgccggc cgcgcgcagc
     1501 gctgcgcgca agtcggccag tgaggtgctc gcgctcaatg gcggaacggc tcagcggcgc
     1561 gccggctgga tgagctcgat gcggttgcct tcggggtcga cgacgaaggc gatgaagatg
     1621 cccgagttcg agaactgcgc ggcttcgcgc gcgacggtgc cgccggccgc tttggctgct
     1681 gccgccgtgg cggcggcatc ggtcaccgtc aaaatcacgt gcatgacggg atcgttcacc
     1741 ggcccttcct tcgtctgcat gatgacgacg gcggggctct ggttggcgcg tgcggcctcg
     1801 gcgttggcgc cgaagttcaa gaacagctcg ggtccgttct gcgtttgcaa ccggttcgtc
     1861 tcgtggaggc cgaaggcggc ggcatagaac ttaccgagcg cctcggcgtc tttcgcggcg
     1921 atgcgcacgg agttcagact gacgtcggcc tgtgcagccg aggcggcgaa tgcaatcagt
     1981 agagcgaagg cgcagcgtag gcgaaccatg gaagtctccc gtcaggtggt cggagtcgcg
     2041 ttggcgccat gccccggtta aaagccctcg agcggggcgg gatcgcggcc gcaagcttac
     2101 gctcttagcg cctcaggctt ccacgggtgg gcttgacgcc atcgacgcac aggaatctga
     2161 tagcagcctg gccctaacaa ttacgacaaa aggggtgacg catggccgaa gcaggtaccg
     2221 tcgacgtcga cggcaagcgc acgacgattc actaccacac gggcgtcgtg acctcgacgc
     2281 gcaagcagaa agaaacgcag attcaaagca
//
LOCUS       DATLEE010000064         4419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_80663, whole genome shotgun sequence.
ACCESSION   DATLEE010000064 DATLEE010000000
VERSION     DATLEE010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80663"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1410
                     /gene="ppnN"
                     /locus_tag="VM692_01350"
     CDS             52..1410
                     /gene="ppnN"
                     /locus_tag="VM692_01350"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HUQ50834.1"
                     /translation="MDALIAPEGSLEILSRVETGHLRDAGEGGLYELWRQCSLAVLNS
                     GASEDDVRKILSRHESFRIAVVQYEGGIALELKNAPDIAFVGNQMIRGIREHLFAVLR
                     DLLYAHDDVLSNPRFDLTSSGGITDAVFHILRNARVLRAHEEPRLVVCWGGHAIEREE
                     YDYTKVVGYALGLEGLDICTGCGAGAMKGPMKGATVGHAKQRIRNGRYLGLTEPTIIA
                     AESPNPICNELVILPDMEKRLEAFVRMAHAIIVFPGGVGTAEEILYLLGILLSPVNAE
                     ERLPVVFTGPASSKTYFERLHTFIGATLGPVAQRRYKVVLGDPAAVAAEVTRGLKLVR
                     DRRAADDNAYFFNWALDIGLEFQKPFEATHEAMRMLELHRDQPAHSLAFNLRRAFSGI
                     VAGNVREAGIARVEKYGPFELRGDAKIIAALGNLLQDFVTERRMRLSDPSAYVPCYRI
                     VA"
     gene            1553..1789
                     /locus_tag="VM692_01355"
     CDS             1553..1789
                     /locus_tag="VM692_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50835.1"
                     /translation="MSDQKPHQFRPALHSKENGGAEEQRANNEQHGWDAYRKWLSRVS
                     TKSTGERTPLDHSIYSWRGYHTWADKVRQSWKDG"
     gene            complement(1786..2565)
                     /locus_tag="VM692_01360"
     CDS             complement(1786..2565)
                     /locus_tag="VM692_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HUQ50836.1"
                     /translation="MKKIVVLNPKGGSGKTTIASNLAACYAVHGERPALMDLDPQASS
                     MRWLRKRPDDEPAIHGIAAFETSATVTRSWQMRIPAECATVVMDTPAAVDPHTLPDLT
                     RGVDAIIVPVMPSDIDIHATAKCIADLLLVAKIRRSERRIGIIANRVRSNTRVSQSLT
                     RFLQSLDIPLIATLRDTQSYVRSAEIGIGVCEMPRWQVQQELPQWQQILSWLSAPRRS
                     SAVNGTSAARLVSIERPVAEHERAPLAGLATTADRELDASA"
     gene            2916..3182
                     /locus_tag="VM692_01365"
     CDS             2916..3182
                     /locus_tag="VM692_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50837.1"
                     /translation="MEVVLLWLDDLDDVLFSAALVWERLRRLVLQIGLVAAFALAGSE
                     LSAIATHWWPAFSAVAGASVGAWLLGGTLRFIYYRETDDSPTAA"
     gene            3242..>4419
                     /gene="htpG"
                     /locus_tag="VM692_01370"
     CDS             3242..>4419
                     /gene="htpG"
                     /locus_tag="VM692_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020583580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HUQ50838.1"
                     /translation="MANGGPSTFAFQAEIKQLLHLMVHSLYSEREIFLRELISNASDA
                     NDRLRFESLARPELLASDPELAIRVTVDANERLLSVSDNGIGMSREEVIDHLGTIARS
                     GTGQFLERLTGDQRKDAQLIGQFGVGFYSAFIVADEVTVLTRRAEAPADQGVSWVSDG
                     HGEYQVAAIERAERGTTVQLKLKANADEFLRPERLKALIRKYSDHIAFPVVLRAGDEP
                     QETVNRAKALWARPRAEIKDEEYVEFYKHIAHDSDDPLLWSHNRVEGKREYTSLLYVP
                     ATAPFDLWNRESPKGVKLYVQRVFITDQATQFLPLYLRFIRGVVDSSELALNVSRELL
                     QQDEAIGAMRSALTRRVLDMLERLAEDKDKYAKFWPEFGALLKEGLVEDSGNRDRLAK
                     L"
BASE COUNT          833 a         1412 c         1403 g          771 t
ORIGIN      
        1 cccgaaccga cgccgatcga gatccgcccg tagcggccct tacgtgaagc catggacgcg
       61 ctgatcgccc cggagggcag tctcgaaatt ctctcgcgcg tcgagaccgg tcatctgcgt
      121 gacgccggcg agggcggact atacgagctg tggcgccagt gctcgttggc cgtgctcaac
      181 tccggcgcat ccgaagacga cgtccgcaag atactgagcc ggcacgaaag ctttcggatc
      241 gccgtggtcc agtacgaggg cggcatcgcg ctcgagctca agaacgcgcc cgacatcgcg
      301 ttcgtcggta accagatgat ccgcggcatt cgcgagcact tgttcgccgt gctgcgcgac
      361 cttctgtatg cccacgacga tgtcttgagc aatccgcgct tcgacctcac gagcagcggc
      421 ggcataaccg acgccgtgtt ccacatcttg cgtaacgcgc gcgtgctgcg tgcgcacgag
      481 gaaccgcgcc tcgtcgtctg ttggggcggc catgccatcg agcgcgagga gtacgactac
      541 acgaaggtcg tcggctacgc gctcggcctc gaagggctcg acatctgcac gggctgcggc
      601 gccggcgcca tgaaggggcc catgaagggc gcgaccgtcg ggcacgcgaa gcaacgcatc
      661 cgcaacgggc gctaccttgg gctcacggag cccacgatca tcgccgcgga gtcgccgaac
      721 ccgatctgta acgagctcgt gatcctgccc gacatggaga agcgtctcga ggcgttcgtg
      781 cgcatggcac acgcgatcat tgttttcccc ggcggcgtcg gcacggccga ggaaatcctt
      841 tatctgctcg gcatcctgct gagccccgtg aacgccgagg aaaggttgcc cgtcgtgttc
      901 acgggcccgg cgtcgagcaa aacgtatttc gagcgcctgc acacgttcat cggcgccacg
      961 ctcggtcccg tggcgcagcg gcgctacaag gtcgtgctcg gcgatccggc cgccgtggcg
     1021 gcggaggtga cgcgcggcct gaagctcgtg cgcgaccggc gcgccgcaga cgacaacgcc
     1081 tattttttca actgggcgct cgacatcggg ctcgagttcc agaagccgtt tgaagcaacc
     1141 cacgaagcga tgcgcatgct cgagctgcat cgcgaccagc cggcacacag cttggctttc
     1201 aacttgcgcc gcgcgttctc gggcatcgtg gcaggcaacg ttcgcgaggc cggtatcgct
     1261 cgcgtcgaga aatacgggcc gttcgagctg cgaggcgacg cgaaaatcat tgccgcgctc
     1321 ggaaatcttt tgcaggactt cgtcacagaa cgacgcatgc gcttgtcgga cccgagcgcg
     1381 tacgtgccgt gttatcgcat cgtcgcttga gttccgtcgc cgcggctacg ccacgcgggc
     1441 attatcgtca aacaagcgcg ctgcaacgcc cgcggctgtg acttggttcc tcggcgcgtc
     1501 gcgcattacc cagtaggctt cgctcatctg gggacaggga ttgtcagcag acatgagcga
     1561 ccaaaaaccg caccagttcc gtccggcctt gcactcgaaa gaaaatggcg gcgcggaaga
     1621 acagcgcgcc aacaatgagc aacacggctg ggacgcgtac cgcaagtggt tgtcgcgagt
     1681 cagcacgaaa tcgacgggcg agcgcacgcc gctcgaccac tcgatctaca gctggcgtgg
     1741 ctatcacacg tgggcggaca aagtccgcca gtcttggaag gacggctaag cggaagcgtc
     1801 caattcccgg tcggccgtcg tggccagccc cgctagaggc gcacgctcgt gctccgcgac
     1861 gggtcgctcg atcgatacca gtcttgccgc agacgtgcca ttcaccgcgc tagagcggcg
     1921 cggtgccgag agccacgaga gaatctgttg ccactgcggc agctcttgtt gaacctgcca
     1981 gcgcggcatc tcgcaaactc cgatgccgat ctccgccgag cgcacgtagc tctgcgtgtc
     2041 gcgcagcgtc gctatcaacg ggatgtcgag gctctgcagg aaacgcgtca gcgattgcga
     2101 cacacgagta ttgctgcgca ccctgttcgc gatgattccg atgcggcgct cggagcggcg
     2161 gatcttcgcg acgagcagca gatctgcgat gcacttagcc gtcgcatgga tatcgatgtc
     2221 ggacggcatc acgggaacga tgatggcgtc gacaccgcgc gtcagatcgg gaagagtgtg
     2281 cggatccact gcggccggcg tatccatgac gacggtcgcg cactcggcgg ggatccgcat
     2341 ctgccagctg cgcgttacgg tcgcagacgt ctcgaacgca gcgatgccgt gaatggccgg
     2401 ctcatcgtcg ggacgcttgc gcaaccagcg catgctcgag gcttgcgggt cgagatccat
     2461 gagcgccggc cgctcgccgt gcaccgcgta gcatgcggcg agattgctgg cgatcgtggt
     2521 tttgccggag ccacccttcg gattcaggac gacgatcttc ttcattgttg acgtcagatc
     2581 gagcgactca gagacagccg acgagcacac attacaataa gtaaaacgct ttgtcgcctc
     2641 gctccggttc ttgagagaac cttcacagcg accgccgtag tcgatcgtta tagttcattc
     2701 gtcacggcgc aagatcttcg agcaatggac gcaaaagatc acggcgagca agcacacgcc
     2761 aagaataaca tttgcaggct ggaatggatc gctgcggcga tcgacgcggc ccgccgccac
     2821 aaagaagacg agcagcgcga gccgccccgg cggccgacac ctatatcgct cgtaaggatc
     2881 gacagcggcc gccgctaact cgagtcacaa ccgacatgga agtcgtgctc ctctggctgg
     2941 acgatctaga cgacgtcttg ttctctgccg ccctcgtctg ggagcggctg cggcgcctcg
     3001 ttctacaaat cggcctggta gccgcgttcg cgctcgcagg ctccgagctc tcggcgatcg
     3061 ccacgcactg gtggcccgcg ttttccgccg tcgcgggagc aagcgtcggg gcctggctgc
     3121 tcggtggcac gctgcgattc atttattacc gcgagacgga cgattcaccg accgcggctt
     3181 gaaaaaacac cgccagtccc taacctaggc ctgaatccac gagcaactgt gcggggcgcc
     3241 aatggcgaac ggcgggccgt ctactttcgc gtttcaggcc gagatcaaac aattgctgca
     3301 cctcatggtg cactcgctgt acagcgagcg agagatcttc ctccgcgagc tgatctccaa
     3361 cgcgtcggac gcgaacgatc gcctgcgatt cgagagcctc gcgcgaccgg agttgctcgc
     3421 cagcgacccc gagctcgcga tccgcgtgac cgtcgatgcg aacgagcggc tgctcagcgt
     3481 cagcgacaac ggcatcggca tgagccgcga ggaggtcatc gaccatctcg gcacgatcgc
     3541 ccgctccggc acgggacagt tcctcgagcg tttgaccggc gatcagcgta aagatgcgca
     3601 gctgatcggc cagttcggcg tgggtttcta ttcggccttc atcgtcgccg acgaggtgac
     3661 ggtattgacg cgtcgcgccg aggcgccggc agatcaaggc gtctcgtggg tctcggacgg
     3721 ccacggtgaa taccaagtcg cggccatcga gcgagccgag cgcggcacga cggtgcagct
     3781 gaagctcaaa gccaacgccg acgagttcct gcgtcccgag cggctcaaag cgctgattcg
     3841 caaatactcg gaccacatcg cgttccccgt cgtcctgcgg gccggcgacg agccgcagga
     3901 aacggtcaat cgcgccaagg cgctctgggc tcggccgcgc gccgagatca aggacgagga
     3961 gtacgtcgag ttttacaagc atatcgctca tgactcggac gatccgctgc tctggagcca
     4021 caaccgcgtc gaaggcaagc gcgagtacac gagcctgctg tacgtgccgg cgaccgcgcc
     4081 attcgatctt tggaaccgcg agtcgccaaa aggcgtgaag ctgtatgtgc agcgcgtgtt
     4141 catcacggat caggccacgc agttcttgcc tttgtacctg cgcttcattc gcggcgtcgt
     4201 cgattcgagc gagctcgcgt tgaatgtctc gcgcgagctg ctgcagcagg acgaggcgat
     4261 cggcgcgatg cgttccgcgc tgacgcgccg cgtgctcgac atgctcgagc ggctcgcaga
     4321 ggacaaggac aagtacgcga agttttggcc cgagttcggc gcgctgctga aagaagggct
     4381 cgtcgaggac tccggcaatc gcgatcggct tgcgaagct
//
LOCUS       DATLEE010000065         2303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8238796, whole genome shotgun sequence.
ACCESSION   DATLEE010000065 DATLEE010000000
VERSION     DATLEE010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8238796"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VM692_01375"
     CDS             complement(<1..626)
                     /locus_tag="VM692_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUQ50839.1"
                     /translation="MRLLLVEDDPNLSRSLATQLEQAGYSVEKSTDGREGLYYAREYP
                     VDLAIVDLGLPSMSGVELIKQLRADGKEYPILVLTARDRWQDKVEVLKLGADDYVVKP
                     FNVEELLARVDALLRRAGGWAQSALVCGPITLDTRTQEVKVADAKLELTSFEYKLLEY
                     LMLHAGEVLSKTQITEALYSEDFERDSNVIEVFIGRLRRKLDPDNSYKP"
     gene            complement(639..1013)
                     /locus_tag="VM692_01380"
     CDS             complement(639..1013)
                     /locus_tag="VM692_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HUQ50840.1"
                     /translation="MSRRFTSMIERARFTGFMAAGMAAALLVVGDLAAARPRPLSYAD
                     VEQVVEPASPAQRDQLFPSQRGGGISLEQATSMAQGRYQGRVVSARSVQMGERLVHEI
                     RILGDDNRVHIVRIDAQTGSFL"
     gene            complement(1110..1697)
                     /locus_tag="VM692_01385"
     CDS             complement(1110..1697)
                     /locus_tag="VM692_01385"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HUQ50841.1"
                     /translation="MLAKKTLLATALLGFLAVSTAMADGRYNSRGRGNAYGHYRSAPA
                     VVTARVVDVEPMVRYVTVDRPRQECWDEVVRQPVRPYGVAGTTAAGSVVGAAIGRQFG
                     SGNDRDALTVLGAVAGGVVARNHALRNGAGATRDVAVERCEVVSDRVREQVVDGYLVT
                     YRLDGRNYTMRTDRHPGDWVQLAVDPRPVNYRVRY"
     gene            complement(1795..>2303)
                     /locus_tag="VM692_01390"
     CDS             complement(1795..>2303)
                     /locus_tag="VM692_01390"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HUQ50842.1"
                     /translation="STARRSKSRASSVACSTCTTKSIGCQPEIPYCIHRAPLSTHERF
                     VAFLVEKFGGALPTWLSPVQLVVVPVADAQRDYAEQLVRRLRSKLVRAEVAAAGQTLA
                     RSVRDASALKVPNVGIVGRREQADGTVTLRRFGCETQLTLSSDELVRRLLLTIEGRRR
                     DFASLDAA"
BASE COUNT          413 a          771 c          727 g          392 t
ORIGIN      
        1 ggtttgtagg aattatcggg atcgagcttg cggcgcagcc ggccgatgaa tacctctatg
       61 acgttgctgt cgcgctcgaa gtcttcgctg tagagcgctt cggtgatctg cgtcttcgaa
      121 agcacctcgc cggcgtgcag catcaggtac tcgagcagct tgtactcgaa gctcgtgagc
      181 tcgagcttcg cgtcggcgac tttgacttcc tgcgtgcgcg tgtcgagcgt gatcgggccg
      241 cagacgagcg ccgactgtgc ccagccgccg gcgcgccgca gtaacgcatc gacgcgcgcc
      301 aagagctcct cgacgttgaa cggtttcacg acgtagtcgt cggcgccgag cttcaaaacc
      361 tcgactttgt cctgccaccg atcgcgcgcc gtcagcacga ggatcggata ctccttgccg
      421 tcagcgcgca attgcttgat gagctcgacg ccggacatgc tcggtagccc gagatcgacg
      481 attgcgagat cgacggggta ttcgcgcgcg tagtacagac cctcgcggcc gtccgtcgat
      541 ttctcgacgg agtacccggc ttgctcgagc tgggtggcca gcgaacggct caggttcggg
      601 tcgtcttcga cgagcagcag acgcataaca agggtccttt aaagaaagga gcccgtctga
      661 gcgtcgatgc gcacgatgtg cacgcgattg tcgtcaccga gaatgcgaat ctcgtggacg
      721 aggcgctcac ccatctgcac gcttctagcg ctcacgacgc gcccctgata ccgaccttgc
      781 gccatcgacg tcgcctgctc gagtgaaata ccgccgcccc gctgcgaggg aaacagctga
      841 tcgcgttgtg ccggcgatgc cggctcgaca acttgctcga cgtccgcgta ggacagcggg
      901 cgcggccgtg cggcggcaag atcgccgacg acgagcagcg cggcggccat ccccgccgcc
      961 atgaagcccg tgaagcgggc gcgctcgatc atgcttgtaa agcgtctgct catcgttgtc
     1021 gtcacagctt gggatgaagc ctcggtccac gtcaaggaag cactcgaacg aaaccgggcg
     1081 ggccgacggc ccgcccggca aggcgctcgc taataccgca cgcgatagtt cacggggcgc
     1141 ggatcgacgg cgagctgcac ccagtcgccc ggatgccggt ccgtccgcat cgtgtaattg
     1201 cggccgtcga gacgatacgt tacgagatag ccgtcgacga cttgctcgcg gactcgatcg
     1261 ctgacgactt cgcagcgctc gaccgcgaca tcgcgcgtcg ctccggcacc gttacgcagc
     1321 gcatggttac gcgcgacgac accgcccgct acggcgccga gcacggtcag cgcatcgcgg
     1381 tcattgccgc tgccgaactg gcggccgatc gctgcgccga cgacgctgcc ggcggccgtc
     1441 gtgccagcca cgccataggg acgtacgggc tgacgaacga cctcgtccca gcactcctgg
     1501 cgcgggcgat cgaccgtgac gtaacgcacc atcggctcga cgtcgacgac gcgggcggtg
     1561 acgacggccg gcgcacttcg gtagtgaccg tacgcgttac cgcggccgcg gctgttatag
     1621 cggccgtccg ccatagcggt cgacactgcg agaaacccca acaacgcagt agccagtagt
     1681 gttttctttg ccaacatggc atctctcctt cgcatccgat cccacctgca ggatcttgga
     1741 gcgcaggtta gaggccgcgg actgaatcga acctgaatgc gaaaacccta ggatctatgc
     1801 ggcgtcgagg ctggcgaagt cgcgccgccg cccttcgatc gtcagcaaca gccggcgcac
     1861 gagctcgtcg ctcgaaagcg tcaactgcgt ctcgcaaccg aagcgacgca acgtcaccgt
     1921 tccgtccgcc tgctcgcgac ggcctacgat cccgacgttc ggaaccttga gcgcgctcgc
     1981 gtcgcgcacg ctgcgcgcaa gcgtctgacc ggcggcggct acttccgccc ggacgagctt
     2041 cgagcgcagc cggcggacga gctgctcggc atagtcgcgc tgcgcgtcgg ccaccggcac
     2101 gacgactaac tgcacgggcg acagccacgt cggcagcgct ccgccgaact tctccacgag
     2161 gaaggcgacg aaccgttcgt gcgtgctcaa cggcgcgcga tggatgcaat aaggaatctc
     2221 cggctggcag ccgatagact tcgtcgtaca tgtcgagcac gccacggagc tcgctcgcga
     2281 cttgctcctc cgtgcagtag atg
//
LOCUS       DATLEE010000066         8692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6004659, whole genome shotgun sequence.
ACCESSION   DATLEE010000066 DATLEE010000000
VERSION     DATLEE010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6004659"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VM692_01395"
     CDS             <1..501
                     /locus_tag="VM692_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUQ50843.1"
                     /translation="APMVLTLLFAAIAVVLAVIGVYGVLAWTVARRTGEIGVRMALGA
                     RSADVLRMIMTQGARMILAGLVLGTAGALALGRVLASQIPEVAAFDALVLAAAVVALT
                     AAALAASWLPARRAARVDRVTGATRELRPQRVEHAVERVIGAPQHDGVGLDGALRLEH
                     LHQRLG"
     gene            702..5021
                     /locus_tag="VM692_01400"
     CDS             702..5021
                     /locus_tag="VM692_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUQ50844.1"
                     /translation="MLPESFHPAVAGWFRSAFPAATSVQLKAWDAIGGGTHTLISAPT
                     GSGKTLAAFLGVIDLLAKQAAACELRDETQILYISPLKALSNDIEKNLRAPLEGIGRE
                     FAGRMLGEAPIRAAVRTGDTPQSERARMRRTPPHILVTTPESLYILLSTESGRKMLRS
                     VRTVIVDEIHALAGNKRGAHLALSLERLEALCERSPTRVGISATTKPIEVLTSFLTGV
                     TDASCTVIDEGHARDRDLTLELPGSPLEAIMSAEVWTELYDRLAEMVRQHRTTLVFVN
                     TRRHAERAARHLAERLGEDAVTSHHGSLAKEHRLKAEERLKAGQLKALVATASLELGI
                     DIGDVDLVCQLGSPHGISAFLQRVGRSGHGLGRIPKGRLFPLSRDDLVECTAMLASIR
                     RRELDAIKPRPKPLDVLAQQIVAEVGVQEWKVADLHKRFTHAASYQDLALDDFVGVSR
                     MLADGFTTHRGRRSAHLHFDRVNGVLRGRRGAALTAVTNGGAIPDQFDYEVVLLPNEL
                     PVGTLNEDFAFESLAGDVFQLGNTSYRIQKVEAGKVYVTDAHGQPPTMPFWLGEAPGR
                     SDELSTSVGQLNETVAQLLDREGPEGAMRWLMQELTLGESAAKQLIDYLGAAKAALGV
                     LPSPQRVVLERFFDETGDTHLVVHSPFGSRINRAWGLSLRKRFCRRFNFELQAAALED
                     SIVLSLGAVHSFALEDVVHYLHSNTVRDVLTQAVLDAPLFGVYWRWNASTALAVRRFR
                     NGKKQPAQFQRMDAEDLLSTVFPDQLACAENLVGGEREIPDHPLVKQTLEDCLTGAMD
                     IDGLERVLKRLEAGEIEVICRELTSPSPLAQEILGAKPYAFLDDAPAEERRTLAVQAR
                     RYMTPEQAAELGKLDPDAIARVRSEAWPQPMNADELHDALCLLGFVTAAEGQAWAPSF
                     ERLRADGRATAVELPKAGTVWAAAERLPEIDVAFPGAVAAERLAVLGGGIADRDTALR
                     ELVRSRFEALGPVTAAALAEPFGLEADDVLPALLALEQQGTAMRGTFTSEGGTAAPGE
                     EWCERRLLARIHRYTLKRLRNEIEPATLGDFQRFLLHWQGLGGERRQGREALSAVLGE
                     LQGLALPAALWEREVLPARIADYDAPLLDQLCSAGEVVWWRPRPSGAQPGARATTVAT
                     SPIAIVPRGTLPLWRSLGSFEPVQGMSGAAERVVQALRDRGALFFVEIVQASGLLRVQ
                     AEEALGELVARGVVTADSFTGLRAVLTPQSRRRSPVGRGRLRGSSGFDGAGRWALLPP
                     RSVPRTATDPEALEHVAKTLLRRYGVMCRRLLAREPLAPSWRELLPFYRTAEARGEIR
                     GGRFIEPFGGEQFALVEAVEELRRLRRQSDANDWVVLAAADPANLLGLTDEVPRAPVP
                     NRRLVFRNGVAVAARGGAAGEMEWLADLAAGERGHVEALLSTEGKPGVDIATAKSGFW
                     RRR"
     gene            5091..5507
                     /locus_tag="VM692_01405"
     CDS             5091..5507
                     /locus_tag="VM692_01405"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HUQ50845.1"
                     /translation="MAQTLTGGCLCGAVRYKVSADMIFSGKCYCEDCRRTSSSGHGSV
                     YAVPEAAVTVTGKLTEYKKAGGSGQPITRRFCPVCGSRISATATVMPGVLMITASSLD
                     DPEKYTSQMSIYTSRAPTWDRPPADSPMFPEMPPQA"
     gene            complement(5573..6487)
                     /locus_tag="VM692_01410"
     CDS             complement(5573..6487)
                     /locus_tag="VM692_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ50846.1"
                     /translation="MSTRLAIIVACAAALSLHAGAQEAPREATVTSEQLQPGLHVLYG
                     SGGGQVSGNVLVLIGDQGSLVVDTGYPVHVPLYRQEIAKLGGGAVTHVINTHFHDDHS
                     EGNKIFGPAALIVAHSNVRHMLMQDNKINVVRTILDQPAFPPAALPVVTFDDRIELYF
                     GSERLELVHVSPAHTAGDVAVILRGKNVMHTGDVFLPAAYPFSDVDNGGDFEGEIEFC
                     RELLKRIDRDTTIVPGHGRTATYADLEAYIAMLETVRGRLFELIASGATLPQVVAAQP
                     TKEFDAKYGNPTTYFLDRSYKSLGGRSP"
     gene            6647..7045
                     /locus_tag="VM692_01415"
     CDS             6647..7045
                     /locus_tag="VM692_01415"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ50847.1"
                     /translation="MQRTIVSIVGFAVLLATPAVFAHHSFAAQFDANKPVTLEGTVTN
                     VEWRNPHIWVYLDVKGADGAVTAWECEGGAPNALTRLGWNRETLRLGGKLTIQGYLAK
                     DGSNVCNARTWTYDGKTVFAGSADGGPTPR"
     gene            7092..7919
                     /locus_tag="VM692_01420"
     CDS             7092..7919
                     /locus_tag="VM692_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50848.1"
                     /translation="MSFTNRFAIAATAAAAFAAPSLASAQGAVIAPPSSPVLAAQPKP
                     DGTPVPRTPDGRVDLSGVWNKSIHQNTAAPVEPLPFTPAGLEAFNAVDDIIDPTARCI
                     LPGVPRVNNSPYPMQIVQLPDRVVFVYEYMHNFRVVYTDGRGHRESWEPSLMGDSIGR
                     WEGETLVIDTVNLTDRTRLDDHGNVHSDALHVIERWRRISANELWYEATLDDPKFYTK
                     PWTTGYVIPLAPRDWEIMEYACTDNNKDLYDGHLQPGSLDGSGRDGTALATPPVPRR"
     gene            complement(7970..>8692)
                     /gene="tdh"
                     /locus_tag="VM692_01425"
     CDS             complement(7970..>8692)
                     /gene="tdh"
                     /locus_tag="VM692_01425"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018691625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HUQ50849.1"
                     /translation="HLCRNTVGVGVDRQGCFAEYLAIPGSNAFKLPDAVTDDIASILD
                     PFGNATHTALAFNLVGEDVLITGAGPIGIMAVAIAKRVGARHVVITDVNDYRLALAQK
                     MGATRAVNVTRQTLDDVMHELGMQEGFDVGLEMSGNPSAFRDMLRTMHHGGSVALLGI
                     PPGDMAVDWRQVIFKGLNMRGIYGREMFETWYKMAALLQAGLDITPVITHHFPIAEFQ
                     RGFETMGSGQSGKVILDWTTLN"
BASE COUNT         1367 a         2886 c         3016 g         1423 t
ORIGIN      
        1 gcgccgatgg tgctgacgtt gctgttcgcg gcgatcgcag tcgtgctcgc cgtcattggc
       61 gtttacggcg tccttgcgtg gaccgtcgcg cggcgcacag gagagatagg cgtgcgcatg
      121 gcgcttggcg cacgcagcgc cgacgttctg cgcatgatca tgactcaagg cgcgcgcatg
      181 atcctcgcag ggctcgttct cggcacggcg ggtgcgttgg cgctcggccg ggtgctggct
      241 tcgcagattc ccgaagtcgc ggccttcgat gcgctcgtgc tggccgccgc cgtcgtcgcg
      301 ctgacggctg cggcattggc cgccagttgg ctgcccgctc ggcgcgccgc gcgcgtcgac
      361 cgcgttacgg gcgctacgcg agaactaaga ccgcagcgcg tcgagcacgc tgtcgagcgc
      421 gtcattggcg cgccgcagca cgacggcgtt ggcctcgatg gcgcgctgcg cctcgagcat
      481 ttgcaccagc ggctcggcta gctccgtcgg ctctgcgcgc tcgacgggcg tggtggcgat
      541 ctcgtgggcg gcctgttgga gcttgcggag attcatctga atcccgtcaa gagctgtcgt
      601 gttgatgccg gtaatcttgt ccatgccggg ccagacggcc gcgaacggca aaattttagc
      661 gccactggac ataaacacag gctttctgta aaacctagac gatgctcccc gagtccttcc
      721 accccgccgt cgccggctgg ttccgcagcg ctttccctgc ggccacgagc gtgcagctca
      781 aagcctggga tgcgatcggc ggcggcacac acacgttgat cagcgcgccg acgggctcgg
      841 gcaagactct cgcggcgttc ctcggcgtca tcgatttgtt ggccaagcag gcggcggcct
      901 gcgagctgcg cgacgagacg caaatcctct acatctcgcc gttgaaggcg ctgtcgaacg
      961 acatcgagaa aaatttgcgc gcgccgctcg aaggcatcgg ccgcgagttc gccgggcgca
     1021 tgctcggcga ggcgccgatc agggctgcgg tacgcacggg cgacacgccg cagagcgagc
     1081 gcgcgcgcat gcggcgcacg ccgccgcata ttctcgtcac gacaccggag tcgctgtaca
     1141 tcctgctcag cacggagtcc gggcggaaga tgctgcgcag cgtgcggacc gtcatcgtcg
     1201 acgagatcca cgcgctcgcc ggcaacaagc gcggcgcgca tctcgccttg tcgctcgagc
     1261 ggctcgaggc gctctgcgag cgctcgccga cgcgcgtcgg catttcggcg acgacgaagc
     1321 cgatcgaagt gctgacgagc tttctcacgg gtgttacgga cgcctcgtgc acggtcatcg
     1381 acgagggcca cgcccgcgac cgcgacctga cgctcgagct gccgggctcg ccgctcgaag
     1441 ccatcatgtc ggcggaggtt tggaccgagc tctacgaccg gctcgcggag atggtgcgcc
     1501 agcaccgcac gacgctggtc ttcgtcaata cgcgccgcca cgccgagcgc gccgcgcgcc
     1561 atctcgccga gcggctcggc gaggacgccg tcacgtcgca tcacggtagc ctcgcgaagg
     1621 agcaccggct caaagccgag gagcgcttga aggccgggca gttgaaggcg ctcgtggcga
     1681 cggcgtcgct cgagctcggt atcgacatcg gcgacgtcga cttggtgtgt cagctcggct
     1741 cgccgcacgg catttccgcg ttcctgcagc gcgtcggccg ctcgggccac gggctcggcc
     1801 gaattcccaa agggcgcctg tttccgctgt cgcgcgacga tctcgtcgag tgcacggcga
     1861 tgctcgcgag cattcgccgc cgcgagctcg acgccatcaa gccgcggccg aagccgctcg
     1921 acgtgttggc gcagcagatc gtcgcagagg tcggcgtcca ggaatggaag gtcgccgacc
     1981 tgcacaagcg tttcacgcac gcggccagct accaagattt ggcgctcgac gacttcgtcg
     2041 gcgtgtcgcg catgctggcc gacggcttca cgacgcaccg cggccggcgc agcgcgcacc
     2101 tgcacttcga ccgcgtcaac ggcgtcttgc gcggccggcg cggcgccgcg ttgacggccg
     2161 tcacgaacgg cggcgcgatt ccggatcagt tcgactacga agtcgtgctg ttgccgaacg
     2221 agctgcccgt cggcacgctg aacgaggact tcgcgttcga gagcttggca ggcgacgtgt
     2281 tccagctcgg caacacgtcg tatcggatcc agaaagtcga ggcgggcaag gtctacgtga
     2341 ccgacgcgca cggacagccg ccaacgatgc ccttctggct cggcgaggcg ccgggccgca
     2401 gcgacgagct gtcgacgtcg gtcgggcagc tcaacgagac cgtggcccag ctgctcgacc
     2461 gtgagggccc cgagggcgcg atgcgctggc tcatgcagga gctcacgctc ggcgagagcg
     2521 ctgcgaagca gctcatcgac tacctcggcg ccgccaaggc ggcgctcggc gtgctgccgt
     2581 cgccgcagcg cgtcgtcctc gagcgtttct tcgacgagac cggcgacacg cacctcgtcg
     2641 tgcattcgcc gttcggctcg cgaatcaacc gcgcgtgggg cttgagcctg cgtaagcgct
     2701 tctgccggcg cttcaatttc gagctgcaag ccgcggcgct cgaggactcg atcgtgttgt
     2761 cgctcggcgc cgtgcacagc ttcgctctcg aggacgtcgt gcactactta cacagcaaca
     2821 ccgtgcgcga cgtgttgacg caagccgtgc tcgacgcgcc gttgttcgga gtctattggc
     2881 gttggaacgc gagcacggcg ctcgccgtgc gccggttccg caacggcaag aagcagccgg
     2941 cacaattcca gcgaatggac gccgaagacc tgctgtcgac ggtgttcccg gatcagctcg
     3001 cctgcgccga aaacttggtc ggcggcgaac gcgagattcc cgaccacccg ctcgtcaagc
     3061 aaacgctcga ggattgcctg acgggcgcca tggacatcga tggcctcgag cgcgtgctga
     3121 agcgcctcga agccggcgag atcgaggtca tctgccgcga gctgacgagc ccctcgccgc
     3181 ttgcgcaaga gatcttgggc gcgaagccgt atgcgttcct cgacgacgcg ccggccgagg
     3241 agcgccgtac tttggccgtg caggctcgcc gctacatgac gcccgagcaa gcggcggagc
     3301 tcggcaagct cgacccggac gcgatcgcgc gcgtgcgctc ggaagcatgg ccgcagccca
     3361 tgaacgccga cgagctgcac gatgcgctct gcttgcttgg cttcgtcacg gcagccgagg
     3421 gtcaagcgtg ggcgccatcg ttcgagcgct tacgcgccga cggccgcgcg acggccgtcg
     3481 agctgccgaa ggccggcacc gtttgggccg ctgccgagcg cttgcccgag atcgacgtcg
     3541 cgttccccgg cgccgtcgcg gccgagcggc tggccgtgct cggcggtgga atcgccgacc
     3601 gcgacaccgc gttgcgcgag ctcgtgcgca gccggttcga ggcgctcggc cccgtcacgg
     3661 ccgcggcact cgcggagccg ttcgggctcg aggccgacga cgtgctgccg gccttgctcg
     3721 cgctcgagca gcagggcacg gcgatgcgcg gcacgttcac gtcggaaggg ggcacggcag
     3781 cgccgggcga ggagtggtgc gagcgccggc tgctcgcgcg catccatcgc tacacgctga
     3841 agcggctgcg caacgagatc gagcccgcga cgctcggcga ctttcagcgc ttcctgctcc
     3901 actggcaggg tctcggcggc gagcgccgcc aaggtcgcga ggcattgagc gccgtgctcg
     3961 gcgagctgca aggtcttgcg ctgccggccg cgctttggga gcgcgaggtg ctgcccgcgc
     4021 gcattgccga ttacgacgcg ccgctgctcg atcagctttg ctcggcgggc gaggtcgtct
     4081 ggtggcggcc gcggccgagc ggcgcgcaac ccggtgcgcg cgcgacgacg gttgcgacgt
     4141 cgcctatcgc gatcgtgccg cgcggtactt tgccgctgtg gcggtcgctc ggctcgttcg
     4201 agcccgtgca gggcatgtcc ggtgcggccg aacgcgtcgt gcaagcgctg cgcgaccgcg
     4261 gcgcgttgtt cttcgtcgag atcgtgcaag cgagcggcct gctgcgcgtg caagccgagg
     4321 aagcgctcgg cgagctcgtc gcgcgcggcg tcgtcacggc cgactcgttt acggggctgc
     4381 gggccgtgtt gacgccgcag agccggcgcc gtagcccggt cggccgcggg cgcttgcgcg
     4441 gcagcagcgg cttcgacggc gccggtcgct gggccttact gccgccgcgc agcgtgccac
     4501 ggacggcgac ggaccccgag gcgctcgagc acgttgcgaa aacgttgctg cgacgttacg
     4561 gcgtcatgtg tcgccgcttg ctcgcccgcg agccgctggc accgagctgg cgcgagctgc
     4621 tgccgttcta ccgtacggcc gaagcgcgcg gcgagatccg cggcggccgc ttcatcgagc
     4681 cgttcggcgg cgagcagttc gccctcgtcg aggccgtgga ggagctgcgg cgcttgcgcc
     4741 gtcagagcga cgcgaacgac tgggtcgtgc tggcggccgc cgatccggcg aatctgctcg
     4801 gcttgacgga cgaggtgccg cgcgcacccg tgccgaaccg ccgcctcgtt ttccgcaacg
     4861 gcgtggccgt agccgcgcgc ggtggggccg ccggcgaaat ggaatggctc gcggaccttg
     4921 cggccggtga acgcggccac gtcgaagccc tgctgtcgac ggaaggcaag cccggcgtcg
     4981 acattgccac tgcaaaaagc gggttttggc gcagaagata accgccgcgg ccgttgcccg
     5041 gcataattgg gcatcccggt ttccggtcac ttacaagaaa ggagagctcg atggcacaaa
     5101 ccctcaccgg cggctgtctc tgcggcgccg tccgttacaa ggtctcggca gacatgatct
     5161 tcagcggcaa gtgctattgc gaggattgcc gcaggacgag cagctcgggc cacgggtccg
     5221 tgtatgccgt gcccgaagcg gccgttaccg tcacaggcaa gctcacggaa tacaagaagg
     5281 cgggcggcag cggccagccg atcacgcgcc gcttttgtcc ggtctgcggg tcgcgtattt
     5341 ccgcgaccgc cacggtcatg ccgggcgtcc tcatgatcac agcgtcgagc ctcgacgacc
     5401 ccgagaagta cacgtcgcag atgagcatct acacgtcgcg tgcaccgacc tgggatcgtc
     5461 cgcccgctga ctcgccgatg ttcccggaga tgccaccgca agcgtgacag atgcggtagc
     5521 gacgctcgac ggcgacgctt tttcgccact gccgacgcat ggggggtacc ggctaaggcg
     5581 agcggccgcc taggctcttg tacgaacggt cgaggaagta cgtcgtaggg ttgccgtact
     5641 tcgcgtcgaa ctctttcgtc ggctgcgcgg cgacgacctg cgggagcgtc gcgccgctcg
     5701 cgattagctc gaagagccgg ccgcgcacgg tctcgagcat cgcaatgtac gcctcgagat
     5761 cggcgtaggt cgcggtgcgg ccgtggcctg gcacgatggt cgtgtcgcga tcgatgcgct
     5821 tcagcagctc gcggcagaac tcgatctcgc cctcgaagtc gccgccgttg tcgacgtcgg
     5881 agaacgggta ggcggccggc aaaaagacgt cgccggtgtg catgacgttc ttgccgcgca
     5941 ggatcacggc gacgtcgccg gccgtatgag ccggcgacac gtgcacgagc tcgaggcgct
     6001 cgctgccgaa gtacagctcg atgcggtcgt cgaacgtgac gacgggcagc gctgcgggcg
     6061 gaaaagccgg ttgatcgagg atcgtgcgca cgacgttgat cttgttgtcc tgcatcaaca
     6121 tgtggcgcac gttgctgtgg gcgacgatca gcgcggcggg gccgaagatc ttgttgccct
     6181 cggagtgatc gtcgtgaaag tgcgtgttga tcacgtgcgt cacggcgccg ccgccgagct
     6241 tggcgatctc ctgacgatat agcggcacgt gcacgggata gccggtgtcg acgacgagtg
     6301 agccttggtc gccgatcagc acgagcacgt tgcccgagac ttgaccgccg ccgctgccgt
     6361 acaggacgtg caggcccggc tgcagctgct cgctcgtaac ggtcgcttcg cgcggcgcct
     6421 cttgcgcgcc ggcgtgtagc gagagggcgg ccgcgcaggc cacgatgatt gcgagacgag
     6481 tgctcatgga ttgcacgccc ttgtaaccgc gcgcttgcga acatgtcaat cgacacgagg
     6541 agaccttcgt gctagctcgg cggttcctcg aaggcgcagc gggcgactat gatggcctac
     6601 cggcacgctt agcgcgcctg agggtggccg agagaagagg ggtagcatgc aacgcacaat
     6661 cgtttcgatc gtcggcttcg cggtgctgct cgcaacgcct gccgtgttcg cgcatcactc
     6721 gttcgccgca caattcgatg ccaacaagcc ggtaacactc gaaggcaccg tcaccaacgt
     6781 cgagtggcgc aaccctcaca tctgggtcta cctcgacgtc aagggcgcag acggggccgt
     6841 cactgcttgg gagtgcgaag gcggggcgcc gaatgctctg acgaggttgg gttggaaccg
     6901 cgaaacactg cggctcggcg gcaagctgac gatccaaggc taccttgcca aggacggcag
     6961 caacgtgtgc aacgcgcgca cgtggaccta cgacggtaag accgtgttcg cgggctccgc
     7021 ggacggcggt ccgacgccgc gctgagcggg cccgctccat cgagagcgct ttcgcaacag
     7081 ggggaatcga cgtgagcttc acgaacaggt tcgccatcgc ggcgaccgca gccgcagcgt
     7141 tcgccgcgcc gtcgctggcg agcgcacaag gggccgtgat cgccccgcca tcgtcgccgg
     7201 ttctcgcggc gcaaccgaag ccggacggca cgcccgtccc gcgcacgccg gacgggcggg
     7261 tggacctatc gggtgtctgg aacaaatcga ttcaccagaa caccgccgcg cccgtggagc
     7321 cactgccgtt cacaccggca ggcctcgagg cgttcaacgc ggtcgacgac atcatcgacc
     7381 cgacggcccg ctgcattctt cccggcgtgc cgcgagtcaa caactcgccg tacccgatgc
     7441 agatcgtgca actgccggat cgcgtcgtgt tcgtctacga gtacatgcac aactttcgcg
     7501 tcgtctacac ggatggccgc ggccaccgcg agagctggga gccgtctctg atgggcgact
     7561 cgatcggccg ctgggagggc gagacgctcg tgatcgacac ggtgaacctg accgatcgca
     7621 cgcggctcga cgatcacggc aatgtccaca gcgacgcgct gcacgtcatc gagcgctggc
     7681 gccgcatcag cgccaacgag ctctggtacg aggcgacgct cgacgatccg aagttctata
     7741 ccaagccgtg gacgacgggc tatgtgattc cgttggcgcc gcgcgactgg gagatcatgg
     7801 aatacgcttg caccgacaac aacaaagatt tgtacgacgg ccacctgcag cctgggtcgc
     7861 tcgatgggtc gggtcgggac ggcacggcgc tggcgacgcc gccggtcccg cgacggtgaa
     7921 gcgctagcga cgctgcgact ccgtcacacg tttcgggcgc gcgcgctcgt taattcagcg
     7981 tcgtccagtc gaggatgact ttgcccgact ggccggagcc catcgtctcg aagccgcgct
     8041 ggaactcggc gatgggaaag tggtgcgtga tcacgggtgt gatgtcgagc cccgcttgca
     8101 gcaacgccgc catcttgtac cacgtctcga acatctcgcg cccgtagatg ccgcgcatgt
     8161 tgagcccctt gaagatcact tgccgccagt cgacggccat gtcgccgggc gggatgccga
     8221 gcaatgcgac gctgccgccg tggtgcatcg tgcgaagcat gtcgcggaac gccgaggggt
     8281 tgcccgacat ctcgaggccg acgtcgaatc cctcctgcat gccgagctcg tgcatgacgt
     8341 cgtcgagcgt ttggcgcgtg acgttgacgg cgcgcgtcgc gcccattttc tgcgcgagcg
     8401 cgaggcgata gtcgttgacg tcggtgatga cgacgtgccg cgcgccgacg cgcttggcga
     8461 tggcgacggc catgatgccg atcggccctg cgcccgtaat caggacatcc tcgccgacca
     8521 agttgaacgc gagcgccgtg tgcgtcgcgt tgccgaacgg gtcgaggatg ctcgcgatgt
     8581 cgtcggtgac ggcgtcgggc agcttgaatg cgttgctgcc gggaatcgcg aggtattccg
     8641 cgaagcagcc ctggcggtcc acgccgactc cgaccgtgtt gcggcacaga tg
//
LOCUS       DATLEE010000067         2715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4554176, whole genome shotgun sequence.
ACCESSION   DATLEE010000067 DATLEE010000000
VERSION     DATLEE010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4554176"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1715
                     /locus_tag="VM692_01430"
     CDS             <1..1715
                     /locus_tag="VM692_01430"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HUQ50850.1"
                     /translation="VLTDMEEITAKVNAATAGKDEKAANDARKAVLTQLEQQCESTAK
                     HKCQAVNLYEGGQYWLYKYKRYTDLRIVFAPEAAIASYGGDPDNFQFPRWCLDFSVLR
                     AYENGRPAATPRHLSIDFAGPAAGDPVFVSGHPGSTDRQLTVAELTTLRDRILPPALL
                     RASELRGRYIEFAKTSESAARIVADPLFGLENGIKVQRKQLDALLDDALLATKRAAEA
                     ELRQRVAGDRSLAAEIGDPWARIEAAQAAEAARYLPLEFLEIGAGFNSRLYRHARSLV
                     RGAAERPKPNAERLREFTDGFLPRLEQQLAAPVPIYPELERLTLSMSLERMREWLGPD
                     APVVRRLLATETPDALAARLVDGSKLADPAVRMQLWNGGQAAIDASTDPFIELVRSLD
                     AEARELRKWHEDEIEAQVEAAHEDIARATFALLGTSVYPDATFTLRLNFGTVQGWVEN
                     GMPVEPFTHLDRLFERATGQPPFAVPAAWQAARERLDLTTPFNLATNSDIVGGNSGSP
                     LIAADGRIVGLLFDGNIHSISGAYWFDEQKNRAVAVHPAVIREALTKVYRAESLLREL
                     GAVR"
     gene            complement(1719..>2715)
                     /locus_tag="VM692_01435"
     CDS             complement(1719..>2715)
                     /locus_tag="VM692_01435"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUQ50851.1"
                     /translation="RMASMSKPVAAVAILMLAEEGKVRLNEPVSRFIPAYANLEVGIA
                     KPVPPGPAPAAQAGPGGPPPAFYKAPATREITVLDLLTHTSGLMSGPMGNSVANAAFN
                     KRHELGLKWVEELGPSPLEFQPGTRWAYSAVAGFDALAHIVEIAAGQEYSAFLRERLF
                     GPLGMRDVTFWPSAEQRSRLATVYQRRDGKLVPGDNPDSMSGEKYHGGGGGIMTTAET
                     YAQFAMMLANGGELNGVRILSPRSVELMGSAFIPSTLPGRPAGEGYGLGVRVVTDAAA
                     RGTWLSDGSFGWSGAYGTHFWVDPKEHLVGILLAQTPNRTFSSDFENAVMQALVD"
BASE COUNT          435 a          965 c          919 g          396 t
ORIGIN      
        1 acgtgctcac cgacatggaa gaaatcaccg ccaaggtgaa cgccgccacg gccggcaagg
       61 acgagaaggc cgcgaacgac gcgcgcaagg ccgtgctcac gcagctcgag cagcaatgcg
      121 aatcgaccgc gaagcacaag tgtcaggcgg tgaaccttta cgagggcggc cagtactggc
      181 tctacaaata caagcgctat accgacttgc gcatcgtgtt cgcgcccgag gccgccatcg
      241 cctcgtacgg cggcgacccc gacaacttcc agttcccgcg ctggtgcctc gatttctccg
      301 tgctgcgcgc ctacgagaac ggccggccgg ccgcgacgcc gcggcacctc tcgatcgact
      361 tcgcgggccc agcggccggc gaccccgtgt tcgtctcggg tcatcccggc tcgaccgacc
      421 ggcaactgac ggtcgccgag ctcacgacgc tacgcgaccg catcctgccg ccggccctat
      481 tgcgcgcgtc ggagctccgc ggccgctaca tcgagttcgc caagaccagc gagtccgcgg
      541 cgcggatcgt cgccgatccg ttgttcggtc tcgaaaacgg catcaaagtc cagcgcaagc
      601 agctcgacgc gctgctcgac gatgcgctgc tcgctaccaa gcgcgcggcc gaagccgagt
      661 tgcggcaacg cgtcgccggc gaccgctcgc tggccgcgga gatcggcgac ccgtgggcgc
      721 gcatcgaggc ggcccaggcg gcggaagccg cacgctatct gccgctcgag ttcctcgaga
      781 tcggcgccgg cttcaacagc cggctctacc gtcacgcgcg ctcgctcgtg cgcggcgccg
      841 ccgagcggcc caagccgaat gcggagcggc tgcgcgagtt tacggacggc ttcctgccgc
      901 gcctcgagca gcagcttgcg gcgcccgtgc cgatctaccc cgagctcgag cggctgacgc
      961 tttcgatgtc gctcgagcgc atgcgcgaat ggctcgggcc cgacgcgccc gtcgtacgcc
     1021 gcctgctcgc caccgagacg cctgacgcgc tcgccgcgcg gctcgtcgac ggctcgaagc
     1081 tcgcggaccc ggccgtgcgc atgcagctgt ggaacggcgg ccaagccgcg atcgacgctt
     1141 cgacggatcc gttcatcgag ctcgtacgct cgctcgacgc cgaagcacgc gagctgcgca
     1201 agtggcacga ggacgagatc gaggcgcaag tcgaagccgc gcacgaggac atcgcgcgcg
     1261 cgaccttcgc gctgctcggg acgagcgtct atcccgacgc cacgttcacg ctgcggctga
     1321 acttcggcac cgtgcaaggc tgggtcgaga acggcatgcc cgtcgagccg ttcacgcatc
     1381 tcgaccgttt gttcgagcgt gccaccggcc agccgccatt cgccgtgccg gccgcctggc
     1441 aggctgcgcg cgagcggttg gatctgacca cgccgttcaa cctcgcgacc aacagcgaca
     1501 tcgtcggcgg caactcgggc agcccgttga tcgccgccga cggtcgcatc gtcggcctgc
     1561 tgttcgacgg caacatccac tcgatctcgg gcgcgtactg gttcgacgag cagaagaacc
     1621 gcgccgtagc ggtgcatccc gcagtcattc gcgaggcgct gacgaaggtg taccgcgccg
     1681 agtccctgct ccgggagctc ggcgcggtgc gttgagccct agtcgacgag cgcctgcatg
     1741 acggcgttct cgaagtccga cgagaacgtc cgattcggag tctgcgcgag caggatgccg
     1801 acgaggtgct ccttcgggtc gacccagaag tgcgtgccgt aggcgccgct ccagccgaac
     1861 gagccgtccg agagccaggt gccgcgcgcg gccgcgtcgg tcacgacgcg cacgcctaag
     1921 ccgtagccct cgccggcggg ccggcccggc agcgtcgacg ggatgaacgc cgagcccatg
     1981 agctcgacgg agcgcgggct caagatgcgc acgccgttca gctcgccgcc gttcgcgagc
     2041 atcatcgcga actgcgcgta cgtctcggcc gtcgtcatga tgccgccgcc gccgccgtga
     2101 tacttctcgc cggacatcga gtcggggttg tcgccgggga cgagcttgcc gtcgcggcgc
     2161 tgataaaccg tcgcgagccg cgagcgctgc tcggcgctcg gccagaacgt cacgtcgcgc
     2221 atgccgagcg gcccaaagag ccgctcgcgc aggaacgccg agtactcctg ccccgcggcg
     2281 atctcgacga tgtgcgccag cgcgtcgaag cccgcgacgg cgctgtaagc ccagcgcgtg
     2341 ccgggctgga actcgagcgg cgacggaccg agctcctcga cccacttcaa gccgagctcg
     2401 tggcgtttgt tgaatgcggc gttggcgacg gaattgccca tcgggccgct catgagaccc
     2461 gacgtgtgcg tcagtagatc gagcacggtt atctcgcgcg tcgcaggagc cttgtagaac
     2521 gcgggcggag ggccgccggg gcccgcttgc gcggcaggcg ccggaccggg cggcacgggt
     2581 ttcgcgatgc cgacctcgag gttagcgtac gcggggatga agcgcgacac gggttcgttg
     2641 aggcgtacct tgccttcctc ggccagcatc aagatggcaa cggctgcgac gggcttcgac
     2701 atcgacgcca tgcgg
//
LOCUS       DATLEE010000068         3882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_87452, whole genome shotgun sequence.
ACCESSION   DATLEE010000068 DATLEE010000000
VERSION     DATLEE010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87452"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2197
                     /locus_tag="VM692_01440"
     CDS             <1..2197
                     /locus_tag="VM692_01440"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HUQ50852.1"
                     /translation="NLSLTTGGVGNGEPVSFDAALDFADEVSGLRGALAANAVVAAAA
                     SGAITATDVETNVTVDAANGAPARTLEATASRIVFDRAAETLAVEGLVTEIAGVRAAW
                     QLEGGTIVTEPTVRGSLQVDPSELATVFEQLGLSLPASLDPADLGTFTLAAQFELQAE
                     PQTVRLSQIDAELLGMHVAGDGALTGRNELAGHVVIAEFTPSATVQTLLRSAVPPTID
                     VGALGPLALDARFDTNLDTRRGALRDFTLTALGAKVSGTLEGIPGERGNVFRGTLRTS
                     RFSAEPMAKAFAALLPANLAANELGMIELDTRFELDTAADTLSLPQLRAEAFGLRASG
                     EVTARNVARAAVWTGTANVAEFSPQDLLQRFGLPPQPTSDAQAFKRATVATRFTVTKD
                     GAQLDDVELKLDETTIKGTFALQGFDARTYRFALDVDRVDADRYLPPKARDAQAGQQT
                     AGDIQLPQNNTMNLDGTMNIAALKLAGMQFSDVGSRIVIGNGDMKLENARARLYGGSF
                     AGNFHVRAAGNDPGLALDGRASGLQLEPLIAALTADAPNFSGTGSFDLNLAGKGRTII
                     ENVQSAGGNVSFDMVGGAIKGFNLGRTLCSAYNVTQRAPSPPELPAVTAYEAIKGSAV
                     VAAGTATSNDLLARTSFMDINGAGSLQLVEQQLDYELDAKLTAPIEIDNCQTLDEFVG
                     GELPFRIKGTVLEPTITPDFSKLIRRQLREGLQDRLEDRLKDRLRGILR"
     gene            complement(2208..2591)
                     /locus_tag="VM692_01445"
     CDS             complement(2208..2591)
                     /locus_tag="VM692_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50853.1"
                     /translation="MSALRRCWLAVATVVPAACGSLAAQERLAVIAASTEQSRAELER
                     VLSAAFDGQPVALAADALTRNSVLAIERRTPPGPQGRAATGRTLEPPVQFKLVLRDTR
                     CMLVRVADAREWPLTEAQCVPAVAR"
     gene            complement(2588..>3882)
                     /locus_tag="VM692_01450"
     CDS             complement(2588..>3882)
                     /locus_tag="VM692_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HUQ50854.1"
                     /translation="EIVDRDPAPYATTTYFDLLRTTATTASGRAKDQFHFTYDTVDWL
                     ALQQSGTEAGYGAVWSVVASLPPRRIVVAYTHPNSPATAPTANLQRGEEVVQVDGVDV
                     VNSNTQAQIDTFVAGLFPDQLNESHSFRLRNPTTGGQRDVTMTSASVTIDPVQNVAVL
                     PTQTGPVGYLHFTDHLLIAEQQLIDAFRSFDQQGVTDLVLDLRYNGGGLLAIASEVAY
                     MIAGNVPTAGQTFERLVFNDKHTSINPITGGALTPMPFFNASSSGQPLPTLDLARVYV
                     LTGGTTCSASESIINGLRGVNVQVIQIGSTTCGKPYGFYPQDNCGTTYFSVEFKGVNA
                     VGFGDYPDGFAPTNRPTSGGERLQGCSVSDDFSLALGNPSEARLNAALEQRLGRACPA
                     PSGAKPSLTKPSNGASDEERDGIATKSPWLMNRWLEPA"
BASE COUNT          639 a         1347 c         1311 g          585 t
ORIGIN      
        1 gaacctgtcc ctgacgacgg gcggcgtcgg caacggcgag ccggtgtcgt tcgacgctgc
       61 gctcgacttc gccgacgagg tatcgggcct acgcggggcc ttggccgcca acgcggtggt
      121 cgcagccgcc gcgagcggcg ccatcacggc gacggacgtc gagacgaacg tgaccgtcga
      181 cgcggccaac ggcgcgccgg cgcgcacgct cgaggcgacc gcgagccgca tcgtcttcga
      241 tcgagcggcc gagacgctcg ctgtcgaagg cctcgtgacg gagatcgccg gcgttcgcgc
      301 ggcgtggcag ctcgagggcg gcacgatcgt cacggagccg acggtgcgcg gcagcctaca
      361 agtcgacccg tccgagctcg cgaccgtgtt cgagcaactg ggactgtcac tgcccgcttc
      421 cctcgatccc gccgacctcg gcacgttcac gctcgccgcg cagttcgagc tacaagcgga
      481 gccgcagacg gtccggctgt cgcagatcga tgccgagctg ctcggcatgc acgtcgcggg
      541 tgacggcgcg ctgaccggcc gcaacgagct cgcgggccac gtcgtcatcg ccgagttcac
      601 gccgagcgcc accgtgcaga cgctgctgcg cagcgccgtg ccgccgacga tcgacgtcgg
      661 cgccttgggc ccgctcgctc tcgacgcgcg cttcgacacg aacctcgaca ctcgccgcgg
      721 cgcgctgcgc gacttcacgt tgacggcgct gggcgccaag gtcagcggca cgctcgaagg
      781 cattcccggc gagcgcggca acgtgttccg cggcacgctg cgcacgtcgc gcttctcggc
      841 cgagcccatg gccaaggcat tcgccgcgct gctgcccgcg aatctcgccg ccaacgagct
      901 cggcatgatc gagctcgaca cgcgcttcga gctcgacacg gccgcggaca ccctgagcct
      961 tccgcagctt cgcgccgagg cgttcggctt gcgcgcgagc ggcgaagtga cggcgcgcaa
     1021 cgtcgcacgc gccgccgtct ggaccggcac ggccaacgtc gccgagttct cgccgcaaga
     1081 cttgctacag cgctttgggc tgccgccgca gccgacttcg gacgcgcagg cgttcaagcg
     1141 cgcgaccgtt gccacgcgtt tcacggtcac gaaagacggc gcgcagctcg acgacgtaga
     1201 gctcaagctc gatgaaacga cgatcaaagg cacgttcgcg ttgcagggct tcgacgcccg
     1261 gacgtatcgc ttcgcgctcg acgtcgatcg agtcgacgcg gaccgctact tgccgccgaa
     1321 agcgcgcgat gcgcaggccg ggcagcaaac cgccggcgac atccagttgc cgcagaacaa
     1381 tacgatgaac ctcgacggca cgatgaacat cgcggcgctg aagcttgccg gcatgcagtt
     1441 ctcggacgtc ggcagccgca tcgtcatcgg caacggcgac atgaagctcg agaacgcgcg
     1501 cgcacggctc tacggcggct cgttcgcagg caactttcat gtgcgcgccg ccggcaacga
     1561 tccggggctc gcgctcgacg ggcgcgcgtc gggcttgcag ctcgagccgc tgatcgccgc
     1621 gctgacggcc gatgcaccca acttcagcgg cacgggcagc ttcgacctca acctggccgg
     1681 caagggccgc acgatcatcg agaacgtgca gtcggcgggc ggcaacgtca gcttcgacat
     1741 ggtcggcggc gcgatcaagg ggttcaatct cggcagaacg ctgtgcagcg cctacaacgt
     1801 cacgcaacgc gcgccctcgc cgccggagct gccggccgta accgcttacg aagccatcaa
     1861 gggctccgcc gtcgtcgcgg cgggaacggc aacgagcaac gacctgctcg cgcgcacgtc
     1921 gttcatggac atcaacggcg cgggctcgct gcagctcgtc gagcagcagc tcgactacga
     1981 gctcgacgca aagctcacgg cgccgatcga gatcgacaac tgccaaacac tcgacgagtt
     2041 cgtcggcggc gagctgccgt tccgcatcaa gggcacggtc ctcgagccga cgatcacgcc
     2101 ggatttcagc aagctcattc gccgccagct gcgcgaaggg cttcaggacc gcctggaaga
     2161 caggctcaag gaccggctgc gcggcattct tcgctgacga ggcgtcgcta gcgcgcgacc
     2221 gcgggcacgc actgtgcctc cgtaagcggc cactctcgtg cgtcggcgac gcgtacgagc
     2281 atgcagcgcg tgtcgcgcag cacgagcttg aactgcacgg gcggctcgag cgtcctgccg
     2341 gtcgctgcgc gcccttgcgg gcccggcggc gtgcgccgct cgatggcgag cacgctgttg
     2401 cgcgtcaacg cgtcggcggc cagggcgacc ggctggccgt cgaacgccgc ggacaacacg
     2461 cgctcgagct ccgcacggct ctgctccgtc gacgcggcaa tgacggcaag tcgttcctgg
     2521 gccgcaagcg agccgcaggc cgccggcact acggtcgcta ctgcgagcca gcagcggcgc
     2581 agcgcgctca cgcgggctcg agccagcgat tcatgagcca cggcgacttg gtcgcaatgc
     2641 cgtcgcgctc ttcgtccgac gcgccgttcg acggcttcgt cagcgacggc ttcgcgcccg
     2701 acggcgcggg acaagcgcgg ccaaggcgct gctcgagcgc ggcgttgagc cgagcctcgc
     2761 tggggttgcc gagggccaag ctgaaatcgt cgctgaccga gcagccttgc agccgttcgc
     2821 cgccggaggt cggcctattc gtcggcgcga aaccgtcggg atagtcgccg aaccccacgg
     2881 cattgacgcc cttgaactcc accgagaagt acgtcgtgcc gcagttgtcc tgcgggtaga
     2941 agccgtacgg cttgccgcaa gtcgtcgagc cgatctgaat gacttgaacg ttgacgccgc
     3001 gcaggccgtt gatgatcgat tcgctcgccg agcacgtcgt gccgcccgtg agcacgtaga
     3061 cgcgcgcgag atccaacgtc ggcagcggct ggcccgagct cgacgcattg aagaacggca
     3121 tcggcgtcaa cgcgccgccc gtgattggat tgatcgacgt gtgcttgtcg ttgaacacga
     3181 gccgctcgaa cgtctgcccc gccgtcggca cgttgccggc gatcatgtag gccacctcgc
     3241 tggcgatcgc cagcaaaccg ccgccgttgt agcgcagatc gagcacgaga tccgtcacac
     3301 cctgctggtc gaacgacctg aatgcgtcga tgagctgctg ctcggcgatc aacagatggt
     3361 ccgtgaagtg caggtagccg accggccccg tctgcgtcgg taggacggcg acgttctgca
     3421 cgggatcgat cgtgacgctc gccgaggtca tcgtgacgtc gcgctgcccg cccgtcgtcg
     3481 ggttgcgcag ccgaaacgag tggctctcgt tgagctggtc cggaaagagg cccgcgacga
     3541 acgtatcgat ctgcgcttgt gtattggaat tgacgacgtc gacgccatcg acttgaacga
     3601 cctcctcgcc gcgctgcaag ttggccgtcg gcgccgtcgc cggcgaattc ggatgcgtat
     3661 aggcgacgac gatgcgccgc ggcggcaagc tggcgacgac ggaccagacc gctccgtagc
     3721 cggcctccgt gcccgactgt tgcagcgcga gccagtccac ggtgtcgtac gtgaagtgga
     3781 attggtcttt ggcgcggccg gacgccgtag tggccgtcgt ccgcagcaag tcgaaatacg
     3841 ttgttgtcgc atacggcgcg gggtcgcggt cgacgatctc gt
//
LOCUS       DATLEE010000069         4708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6751936, whole genome shotgun sequence.
ACCESSION   DATLEE010000069 DATLEE010000000
VERSION     DATLEE010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6751936"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..2452
                     /gene="gyrA"
                     /locus_tag="VM692_01455"
                     /pseudo
     CDS             1..2452
                     /gene="gyrA"
                     /locus_tag="VM692_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786483.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
     gene            2470..3552
                     /gene="serC"
                     /locus_tag="VM692_01460"
     CDS             2470..3552
                     /gene="serC"
                     /locus_tag="VM692_01460"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HUQ50855.1"
                     /translation="MKRVFNFSPGPAMLPEPVLRRAQAELLDWRASGMSVMEVSHRGT
                     DFIELAAQSERALRELLAVPANYKVLFLQGGATLQFAAVPLNIAPVGSVVDYVVTGNW
                     GEKAAQEAQRYVNVNVAANSKASKFTTVPDPRTWQVSSSAAYLHYTPNETVFGVEMHT
                     VPEVSNAPLVVDMSSTLLSRPIDVSRFGIIYAGAQKNIGPAGLAIVIVREDLIGKARR
                     ETPGVIDYKLMAENDSMWNTPSTLSWYLAALVFDWLKEQGGLAAMGKINERKAAKLYA
                     AIDSSGYYKNPVDKAYRSWMNVPFTLPNAELDKPFLSEAKAAGLTNLKGHRVVGGMRA
                     SIYNAMPEAGVDALIAFMGDFAKRHG"
     gene            3558..>4708
                     /locus_tag="VM692_01465"
     CDS             3558..>4708
                     /locus_tag="VM692_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HUQ50856.1"
                     /translation="MATFKIRTLNSIAVAGLDRLPRDRYEVASELAQPDAVLLRSFKM
                     HDMALPESLLAVGRAGAGTNNIPVAKLSKLGVPVFNTPGANANAVKELVIAGLLMAAR
                     NLPQALAYVHTLKGSGDELDKAVEAGKKQFVGFELPKRTLGVVGLGAIGVEVANAALK
                     LGMNVLGYDPTITVQHAWALSSGVQKAGNLDELFARADVVTVHVPLNKSTDKLVNGAR
                     LETMRRGGVILNFSRDAIVDSAAVIEALGNGKLRAYVTDFPARELIDHPKVICLPHLG
                     ASTVEAEENCAVMVVDNVREFLENGNVTRSVNFPETVLQRTRPHRVAIPHRNVPNMVA
                     QILTALAAQNINIADMLNHSRDELSYTLVDLDAPPAEETVAKIRGIDGIL"
BASE COUNT          903 a         1518 c         1505 g          782 t
ORIGIN      
        1 cgtgcgcgac ggcctgaagc cggtacaccg gcgcatcctc tactcgatgc tgggtctcgg
       61 cttgctgccg gaccgcgcgt acaagaagag cgcgacggtg gtgggggacg tgctgggcaa
      121 gtaccacccg cacggcgaca ccgcggtgta cgacgcgctg gtgcgcatgg cgcaggactt
      181 ctcgatgcgc tacatgctcg tcgacgggca gggcaacttc ggctcggtcg acggtgacgc
      241 cccggcggcg atgcgctaca ccgaggtgcg catggcgcgc atcacgcaag agctgctcgc
      301 ggacatcgac aaagaaacgg tcgactttgt cccgaactac gacgagagcg agcgcgagcc
      361 gtcggtattg ccgacgcgaa ttccgaactt gctcgtgaac gggtcgagcg gtattgccgt
      421 cggcatggcg acgaacattc cgccgcacaa cttgggcgag gtgatcgacg cctgtctcgc
      481 gttactgagc aatccctgaa tcgagctggc cgagctgatc aagctcgtcc gaggccccga
      541 cttcccgacg gccgggctga tctacggcat ccaaggcgtc gtcagtgcgt acgcgtcggg
      601 gcgcggacgt atctacgtgc gcgggcgcac tcacgtcgag ccgctcggcg aacgcagcga
      661 tcgcgaagcg atcatcatca cggagctgcc gtaccaagtt aacaaggcgc gtctcatcga
      721 gcgcattgcc gagctcgttc gcgacaagaa aatcgaaggc atcagcgagc tgcgcgacga
      781 gtccgacaag gacggcatgc gcgtcgtcat cgagctgaag cgcggcgaga tcacggacgt
      841 cgtcttgaac aacttgttca agcagacgcc gctgcagagc gtcttcggca tcaacatggt
      901 cggcctcgtc gacaaccagc cgaagctgct gtcgctgaag gagttgctcg aggcgttctt
      961 gcggcatcgc cgcgagatcg tcacgcgccg caccttgttc gacctgcgca aggcgcgcga
     1021 gcgcgcgcac atcttggaag gtttgatggt cgcgctggcg aacatcgacg agatcatcga
     1081 gctcatcaaa gcgtcgccgt cgccggccga ggcacgcgcc gccttgtgcg ccaaggcgtg
     1141 gcgcgcaggc gcggtcacgg ccatgctcga acgcgcgggc gcggccgcca cgcgacccga
     1201 gaacctcacc gaagagtcgg gcctcgagaa cggcacgtac cggctatcga cggcccaagc
     1261 gcaggcgatc ctcgatttgc ggctgcatcg gctgacgggt ctcgagcagg ataaaatctc
     1321 gtcagaatac agcgagttga tcgtcaatat tcaggcattt ctagagattc ttggcaaccc
     1381 cgacaagctg caggaagtcg ttcggaccga gctcacggag gtgcgcgccg cgtacgccga
     1441 caaacgccgc acggagatcg tcgaaaacta cgacgacctc accgtcgagg atctgattcc
     1501 cgaggagacc gtcgtcgtca cgctgtcgca cgcgggctat gcgaagtcgc agccggccac
     1561 cgtctatcaa gcgcagcgcc gcggcggccg cggccgcacg gcggctcgcg tcaaggacga
     1621 agatttcatc gatcaactgt tcgtcgccaa ctcgcacgac acggtgctgt gcttctcgag
     1681 ccgcggcaag gcgtactggc tcaaggtcta ccaggtgccg caagcggcgc ccggtgcacg
     1741 cggccggccg atcgtgaact tgctgccgct cgaggaaggc gagcgcatca acgcgatctt
     1801 gccggtcaag gcgttcgaag aagacaagta cgtgtttctc gcgacgaagc acggcacggt
     1861 aaaaaagacg ccgctcgcgg cattcgcgcg ccagcgcacg agcggcatca tcgccgtcga
     1921 gctgcgcgac gacgatcgcc tcatcggcgc cggcatcacg agcggcaacc aagacatcat
     1981 gctgttcgcg agctccggca aagccatccg cttctcggag caagacgttc ggccgatggg
     2041 ccgcactgca gccggcgtac gcgggctcaa gctcggcggg ccgaccgacg aggtcatcgc
     2101 gctcagcatc atcggggccg gcccggttct gacggccacg gagaacggtt acggcaagtt
     2161 gacgccgatc gacgagcacc cggtccaagg ccgcggcggc cagggcgtca tttgtatcca
     2221 gacctcggag cgcaacggcc gactggtcgg cgcgatgcag gtatcgcccg aggacgaggt
     2281 catgctgatc acttcgacgg gcacgatgat tcgcacgccc gtttccgaca tctcgacgat
     2341 gagccgcaac acgcaaggcg tgcgcttgat tcgcttggac gatagcgatc gactcgtcgg
     2401 catctcacgt gtcgaagcca tgccgggcga cgataccgcg accccggaat agggtacgga
     2461 gaccccccaa tgaagcgtgt tttcaacttc agccccgggc cggcgatgtt gccggaaccg
     2521 gttttgcggc gtgcgcaagc cgagctgctc gactggcgcg ccagcggcat gtccgtcatg
     2581 gaagtcagcc atcgcggcac cgacttcata gagctcgccg cccagtcgga acgtgcgctg
     2641 cgcgagctgc tcgccgtgcc agccaactac aaggtgctgt tcttgcaagg cggcgccact
     2701 ctgcaattcg ccgccgtgcc gctgaacatt gcgcccgtcg gttcggtcgt cgactacgtc
     2761 gtcaccggca attggggcga gaaggccgcc caagaggcgc agcgctacgt caacgtcaac
     2821 gtcgccgcca acagcaaggc ctcgaagttc acgaccgtgc ccgatccgcg cacttggcag
     2881 gtctcgagct cggccgctta tcttcattac acgccgaacg aaaccgtgtt cggcgtcgag
     2941 atgcacaccg tgcccgaagt ctcgaatgcg ccgctcgtcg tcgacatgtc gtcgactcta
     3001 ttatcgcggc cgatcgacgt cagccgcttc ggcatcattt acgccggcgc gcagaagaac
     3061 attggtccgg ccggcctcgc gatcgtcatc gtgcgcgagg atctgatcgg caaggcgcga
     3121 cgcgagacgc cgggtgtcat cgattacaag ctgatggccg aaaacgattc gatgtggaat
     3181 acgccatcga cgttgtcctg gtacttggcg gcgctcgtct tcgactggct gaaggagcag
     3241 ggcggcctcg cggccatggg caagatcaac gagcgcaaag cggcgaagct gtacgcggcg
     3301 atcgattcct cggggtatta caagaatccc gtcgacaagg cctatcgctc gtggatgaac
     3361 gtgccgttca cgctgccgaa cgccgagctc gacaagccgt tcttgtccga agcgaaggcg
     3421 gccgggctca ctaacttgaa agggcaccgc gtcgtcggcg gcatgcgtgc cagcatctac
     3481 aacgcgatgc ccgaggcggg cgtcgacgcg ctgatcgcct tcatgggcga cttcgcgaag
     3541 cggcacgggt gagcgtaatg gcaaccttta aaattcgcac cttgaacagc attgcggttg
     3601 ccggcctcga tcgtttaccg cgcgatcgct acgaggtcgc ctccgagctc gcgcagcccg
     3661 atgccgtgct tttgcggtcg ttcaagatgc acgacatggc gctgccggag tcgttgctgg
     3721 ccgtcggacg cgccggcgcc ggcacgaaca atattccggt cgccaagctc agcaagctcg
     3781 gagtgccggt gttcaatacg ccgggagcca acgccaatgc cgtcaaggag ctcgtcatcg
     3841 cgggcttgct gatggccgca cgaaacttgc cgcaggcgct cgcctacgtc catacgctga
     3901 aaggctccgg cgacgagctc gacaaggccg tcgaagcggg caaaaagcaa ttcgtcggct
     3961 tcgagctgcc gaagcgcacg ctcggcgtcg tcggcctcgg cgccatcggg gtcgaggtcg
     4021 cgaacgccgc gctgaagctc ggcatgaacg tgctgggcta cgatcccacg atcaccgtgc
     4081 agcacgcctg ggcgttgtcg tctggggtgc agaaggccgg caatctcgac gagctgttcg
     4141 cgcgcgccga cgtcgtcacc gtgcacgttc cactgaacaa gtcgacggac aagctcgtca
     4201 acggtgctcg gctcgaaacg atgcgacgcg gcggcgtgat cctgaacttt tcgcgtgacg
     4261 ccatcgtcga ctcggctgcc gtcatcgaag cgctcggcaa cggcaagctg cgcgcgtacg
     4321 tcacggattt cccggcgcgc gagttgatcg atcatccgaa ggtcatctgt ctgccgcacc
     4381 tcggggcgtc gaccgtcgaa gccgaggaga attgcgccgt catggtcgtc gacaacgtgc
     4441 gcgagttcct cgagaacggc aacgtgacgc ggtccgtcaa tttccccgag accgtgctgc
     4501 aacgcacgcg tccgcatcgc gttgcgatcc cgcacaggaa cgtgccgaac atggtcgcgc
     4561 agatcctgac ggcgctcgcg gcgcagaaca tcaacattgc cgacatgctg aaccactcac
     4621 gcgatgagct ctcgtacacg ctcgtcgatc tcgacgcgcc gccggccgaa gaaaccgtcg
     4681 ccaagattcg cgggatcgac ggcatcct
//
LOCUS       DATLEE010000070         3960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1579234, whole genome shotgun sequence.
ACCESSION   DATLEE010000070 DATLEE010000000
VERSION     DATLEE010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579234"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VM692_01470"
     CDS             complement(<1..1073)
                     /locus_tag="VM692_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUQ50857.1"
                     /translation="MNLALPQHESLVHMLLAAVDAQPDLPAIILERQSITYAELGRAV
                     AGLAQRLESAGAKRGERVILLMANSIEMDVALHAVMATGAQVAPINPFLTPSELKKQL
                     GDVQACAVIGDKASEEKASQVAALFDIPTQLTLGAGGITLEAWKADKSLALDRARLPK
                     ADDFALLIFTGGSTGVPKGVNHTHRALLYSVLQHVTVWPCKFGQERFLNVAPMFHIWG
                     LGYSTFVPIYAQSTLVMVPRYDADKVVQGLSDHKITVFAGGPAPIYMGLLASPMFAKA
                     DLSQLKYCPSGGAPCPEELHREWLEKTGQPILEGWGMTEGAPFCLNVYDGKRKLLSVG
                     NPVPGTELQVLDLESGDRVLPQG"
     gene            complement(1111..2346)
                     /locus_tag="VM692_01475"
     CDS             complement(1111..2346)
                     /locus_tag="VM692_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HUQ50858.1"
                     /translation="MSTKPKASFLAVLGPGLLLAATGIGGGDLTTATFVGGMLGTSVL
                     WAVALGAFMKFVITEGLTRWQLATGETLIEGVVKRLGPVVIWIFLPYFLLWSFFVASA
                     QMSSNGIALHALLPLFDDPVHGKIVFGVLASLAGLTLVLRGGYRWFEVAMKIGIGTMF
                     VTIAVTAIALWPGAGEVLRGLFVPTLPRADAEAVTWTVSLIGGIGGTLTVLAYGYWLR
                     EEGRTSSEDLRTCRIDLAASYFVVALFGIFMIIVGQSVKIEGEGTGMLIVLSDRLGQE
                     LGPIGKWLFLIGTTGTVFSSLLGVWQATPYLFAECWRLGVRRDPKPVDTSAPTYRRFL
                     LVLAIVPMLGLFASFREVQKLYTFIGAYIFPTLALVLIVFNSRAAWVGQRFKNHPVTI
                     AVLAGVLAFFTWMALNIDA"
     gene            complement(2420..3427)
                     /locus_tag="VM692_01480"
     CDS             complement(2420..3427)
                     /locus_tag="VM692_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUQ50859.1"
                     /translation="MEYRNLGRTGVKVSPLCLGTMMFGRSADEQTSVAIVNAALERGI
                     NFVDTADAYSAGASERFVGKALQGKRASVVLATKAFFPQDQKDPNARGLSRRHLIAAC
                     EASLTRLQTDWIDLYQVHRPQSDIPIDETLRALDDLIRAGKVRYIGTSMFPAWKSVES
                     LWAAKELGLNRFVCEQMAYNLLDRSAEREVIPAAQSFGMALIPWAPLCGGLLTGKYKR
                     DDQSAAGRWHGGKDNFGRPATQAAFDVIEGLVALANDKGCTPSQLALAWNAAQPGITS
                     PIIGPRTVEQLVDNAGAFAVTVSDADRARLDELAPPWSATLRYYEAALAIDFRPHLAR
                     W"
     gene            complement(3631..>3960)
                     /locus_tag="VM692_01485"
     CDS             complement(3631..>3960)
                     /locus_tag="VM692_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008105678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HUQ50860.1"
                     /translation="VADIGPIVNLSTAENQCQGSVIDALSTAMGLKITFEGGKVEQSN
                     FHQYPIVRIDKAPEIDVHFLDTDYQPTGCGEPAFPPAAPAIANAIFAATGQRVRTLPF
                     SVEGYSV"
BASE COUNT          709 a         1348 c         1278 g          625 t
ORIGIN      
        1 tcaccctggg gcaacacgcg gtcgccgctc tcgaggtcga ggacttgcag ctccgtgccc
       61 ggcacgggat tgccgaccga cagcagcttg cgcttgccgt catagacgtt caggcagaac
      121 ggcgcgccct cggtcatgcc ccaaccctcg aggatcggtt ggcccgtctt ctcgagccat
      181 tcgcgatgca gctcctcggg gcacggcgcg ccgcccgaag ggcagtactt gagctgcgag
      241 agatcggctt tggcgaacat cgggctcgcg agcaagccca tgtagatcgg cgcggggccg
      301 ccggcgaaca ccgtgatctt gtgatcggag agcccttgca cgactttgtc tgcgtcgtag
      361 cgcggcacca tgacgagcgt gctctgcgcg tagatcggca cgaacgtcga gtagccgagg
      421 ccccagatgt ggaacatcgg cgcgacgttg aggaagcgtt cttggccgaa cttgcacggc
      481 cacacggtca cgtgctgcag caccgagtac aacaaggcgc gatgcgtgtg gttgacgcct
      541 ttcggcacac ccgtcgagcc gcccgtgaaa atcagcagcg cgaagtcgtc ggccttcggc
      601 agccgcgcgc gatcgagagc gagcgacttg tcggccttcc acgcctcgag cgtgataccg
      661 ccggccccga gcgtgagctg ggtcggaatg tcgaacaacg cggcgacttg gctcgctttc
      721 tcctcggacg ccttgtcgcc gatgaccgcg cacgcctgca cgtcgccgag ctgcttcttc
      781 agctccgacg gcgtcaggaa aggattgatc ggcgcgacct gcgcgcccgt cgccatgacg
      841 gcatgcagcg cgacgtccat ctcgatcgag ttcgccatca acaggatgac gcgctcgccg
      901 cgcttcgcgc ctgcgctttc caagcgctgc gcgaggccgg cgacggcgcg gccgagctcg
      961 gcgtacgtga tgctttgtcg ctcgaggatg atcgcgggca aatccggctg cgcgtcgacg
     1021 gcggccagca gcatgtgcac gaggctctcg tgctgcggca gcgctaggtt caacggcctc
     1081 ggagcactga cgtgagaggt agcggtcata tcaagcgtcg atatttagag ccatccaggt
     1141 gaagaacgcg agcacaccgg cgagaacggc aatcgtcacc ggatgattct tgaagcgctg
     1201 gcccacccac gcagcgcgcg agttgaagac gatcaacacg agcgcgagcg tcgggaagat
     1261 atacgcgccg atgaaagtat agagcttctg cacttcgcgg aagcttgcga acagaccgag
     1321 catcggcacg attgcgagca ccagcaagaa ccggcgatac gtcggcgcgc tcgtgtcgac
     1381 gggcttcggg tcgcgtcgta cgcccaagcg ccaacactcc gcgaacaagt acggcgtcgc
     1441 ctgccagacg ccgagcaagc tgctgaagac ggtgcccgtc gtgccgatca ggaacagcca
     1501 cttgccgatc ggccccagct cctggccgag ccggtccgac agcacgatca gcatccccgt
     1561 gccctcgcct tcgattttca cgctctggcc gacgatgatc atgaagatgc cgaacagggc
     1621 gacgacgaaa tagctcgctg cgagatcgat acggcaggtg cgcaggtcct cggagctcgt
     1681 gcgaccttcc tctcgcagcc aataaccgta ggccagcacg gtcagcgtgc cgccgatgcc
     1741 gccgatcagc gagacggtcc atgtgacggc ttcggcatcg gcgcgcggca acgtcggcac
     1801 gaacagtccg cgcagcacct cgccggcgcc gggccacaat gcgatggccg tgaccgcgat
     1861 cgtcacgaac atcgtgccga tgccgatctt catcgcgacc tcgaaccagc gatagccgcc
     1921 gcgtagcacg agcgtgagcc cggccaagct cgcgagcacg ccgaacacga tcttgccgtg
     1981 cacggggtcg tcgaacaacg gcaacagcgc gtgcagcgcg atgccgttcg agctcatctg
     2041 cgccgacgcg acgaagaacg accagagcaa aaaatacggc aggaaaatcc agatgaccac
     2101 ggggccgagc cgcttgacga cgccttcgat cagcgtctcg cccgtcgcga gctgccagcg
     2161 cgtgagccct tcggtgatga cgaatttcat gaacgcgccg agcgcgacgg cccaaagcac
     2221 gctcgtgccc agcatgccgc cgacgaaagt cgccgtcgtc agatcgccgc cgccgatgcc
     2281 ggttgcggcg agcagcaggc cgggaccgag cacggccaaa aaagatgcct tgggtttcgt
     2341 cgacatttga tcttggccag cgacggccgg aacggtaaca cgccgcgcgg gcggcgtcag
     2401 ggtcgcggcg gcgcctgcgc taccagcgcg cgagatgcgg gcggaagtcg atggcgagcg
     2461 cggcctcgta gtaacgcagc gtggccgacc acggcggcgc cagctcgtcg agccgggctc
     2521 gatccgcgtc gctgacggtg accgcgaacg cacccgcgtt gtcgacgagc tgctcgacgg
     2581 tgcgcggccc gatgatcggc gacgtgatgc cgggttgcgc cgcgttccac gccagcgcca
     2641 gttgcgacgg cgtgcagccc ttgtcgttcg cgagtgcgac gagcccttcg atgacgtcga
     2701 acgccgcttg cgtcgcgggc cggccgaaat tgtccttgcc gccgtgccag cggcccgccg
     2761 cgctctgatc gtcgcgcttg tacttgccag tcaacaggcc gccgcaaagt ggcgcccacg
     2821 ggatcaacgc catcccgaag ctctgcgccg cggggatgac ttcgcgctcc gcgctgcggt
     2881 cgagcaagtt atacgccatc tgctcgcaaa cgaagcggtt cagcccgagc tccttcgcgg
     2941 cccacagcga ctcgacgctc ttccaagccg ggaacatgct cgtgccgatg tagcgcacct
     3001 tgccggcgcg gatcaggtcg tcgagcgcgc gcagcgtctc gtcgatcgga atgtccgact
     3061 gcggccggtg cacttgatac agatcgatcc agtcggtctg caagcgcgtc agcgatgcct
     3121 cgcacgccgc gatcaggtgg cgccggctca agccgcgcgc attcggatcc ttctgatcct
     3181 gcggaaagaa cgccttcgtc gcgagcacga ccgacgcccg cttgccttgc agggccttac
     3241 ccacaaagcg ctcactcgcg cccgccgagt acgcgtccgc cgtatcgacg aagttaatgc
     3301 cccgctcgag cgcagcgttg actatggcga cggacgtctg ctcgtccgca ctccgcccaa
     3361 acatcatcgt cccaaggcac agcggactga ccttaacgcc agtacgcccg aggttgcggt
     3421 attccatacg cactccgcga tgccgcagag aggccttacc agcgaggggg cctctcgggg
     3481 cgctgcgcgc cagggatggc gcgcagcgag cctacggggg cgtgattcgc tcctgcaatt
     3541 gcggcacgcc gccatccatg gcggcgacgt acttgcggcg tccccggaag cgcccctagc
     3601 aggcgcgcct ccgggacacc gcgcgatgcc ctaaacgctg tagccctcga cgctgaacgg
     3661 cagcgtccgc acgcgctgcc cagtcgccgc gaagatcgcg ttcgcgatgg ccggcgccgc
     3721 cggcgggaac gccggctcgc cgcaacccgt cggctgataa tccgtgtcga ggaagtgcac
     3781 gtcgatctcg ggcgccttgt cgatgcgcac gatcggatac tgatggaagt tcgactgctc
     3841 gaccttgccg ccctcgaacg tgatcttgag ccccatcgcc gtgctcagcg catcgatgac
     3901 cgagccctgg cactgattct cggccgtgct caggttcacg atcggcccga tgtcggcgac
//
LOCUS       DATLEE010000071         2614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_89053, whole genome shotgun sequence.
ACCESSION   DATLEE010000071 DATLEE010000000
VERSION     DATLEE010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89053"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /gene="glmU"
                     /locus_tag="VM692_01490"
     CDS             <1..924
                     /gene="glmU"
                     /locus_tag="VM692_01490"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HUQ50861.1"
                     /translation="EHKDANDEERRIDEINTGLLAYDAAPLRNYLGRLKNDNSQREYY
                     LTDVIAMAVADKVQVQGIVAESPTEVLGINDRAQLAAAERAWQRQQAADLMARGATLA
                     DPERIDIRGEVVVGRDVFIDVGVVLQGKVVVGDRTRIGPYAVITDCTLGADTVVHPHT
                     VMNSSSAGDDCEIGPFSRLRPGTVLAGHVKVGNFVEMKNSQIAPHSKVNHLTYVGDAT
                     VGTEVNVGAGTITCNYDGANKHRTVIGDKAFIGSGCMLVAPVEIGAGATIGAGSTITK
                     NAPDGELTIARSKQVTIPGWQRPKKKPKPGK"
     gene            944..>2614
                     /gene="glmS"
                     /locus_tag="VM692_01495"
     CDS             944..>2614
                     /gene="glmS"
                     /locus_tag="VM692_01495"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HUQ50862.1"
                     /translation="MCGIVGAIAERNIVPVLVEGLKRLEYRGYDSAGLAVVQPDKKLG
                     LRRTVGKVAQLETKLNEKPLAGRLGVAHTRWATHGGVTEANAHPHLSGDRVAVIHNGI
                     IENYEPLKNELIEKGYEFKSETDTEVAAHLIHDYLKQGHDLLEALRLAVARFVGAYAL
                     LVFDAKDPERIVVTRIASPLVIGLGIGENFVASGVPALLPVTQRFIYLEQGDVAEITR
                     DAVKIFDAQGRPVTRAVHETQWNQEAAEKGPYRHFMLKEIYEQPSALADTLYGRVVNK
                     RVVPESLGPRAAELLPKVQNIHIVACGTSYHAGSVGRYWFESIAGVPCQVEIASEYRY
                     RNVVVPPNTLFLTLSQSGEPADTLAALRMAKTAGYLASLTICNSPHSSMVRESDLVMM
                     TQAGPEIGVASTKAFTTQLLSLLLVTLMLGRHRGMSAARESDFVPHLYHAAAAVEQAL
                     QMNELVRLLAEDFAEKHHALFLGRGPMWPIAMEGALKLKEISYIHAEAYAAGELKHGP
                     LALIDEDMPVIVVAPNNELLDKLKSNMQVVRARGGQLYVFADRETGLES"
BASE COUNT          489 a          882 c          859 g          384 t
ORIGIN      
        1 gagcacaagg acgcgaacga cgaggagcgg cgcatcgacg agatcaacac gggtctgctc
       61 gcctacgacg cggcgccgtt gcgcaactac ctcggccgct tgaagaacga caactcgcag
      121 cgcgagtact acttgaccga cgtcatcgcg atggcggtcg ccgacaaggt ccaagtgcag
      181 ggcatcgtcg ccgagtcccc gaccgaagtg ctcggcatca acgatcgcgc gcagctcgcc
      241 gccgccgagc gcgcgtggca acggcagcaa gccgccgacc tcatggcgcg cggcgcgacg
      301 ctcgcggacc ccgagcgcat cgacatccgc ggcgaggtcg tcgtcggccg cgacgtgttc
      361 atcgacgtcg gcgtcgtgct ccaaggcaag gtcgtagtcg gcgatcgcac tcgcatcggg
      421 ccgtacgccg tcattaccga ttgcacgctc ggcgccgaca cggtcgtgca cccgcacacg
      481 gtcatgaact cgagctccgc cggcgacgac tgcgagatcg ggccgttctc gcggttgcgg
      541 cccggcaccg tgctcgcagg gcacgtcaag gtcggcaact ttgtcgagat gaagaacagc
      601 cagatcgcgc cgcacagcaa ggtcaatcac ctcacgtacg tcggcgacgc aaccgtcggc
      661 accgaggtca acgtcggcgc cggcaccatc acctgtaact acgacggcgc caacaagcat
      721 cgcacggtca tcggcgacaa ggcgttcatc ggctcgggct gcatgctcgt ggcgcccgtc
      781 gagatcggcg caggtgcgac gatcggcgca ggctcgacga tcacgaagaa tgcgccggac
      841 ggcgagctca cgatcgcgcg ctccaagcag gtcacgatcc cgggttggca gcggcccaag
      901 aaaaagccca agccaggcaa gtaagtaagg acacagcaaa cctatgtgcg gcatcgtcgg
      961 agctatcgca gagcgcaaca tcgttcccgt gctcgtcgag ggcttgaagc gcctcgagta
     1021 ccgcggttac gactcggcgg ggctcgccgt cgtgcagccg gacaagaagc tcgggctgcg
     1081 gcgcaccgtc ggcaaggtcg cgcaactcga gaccaagctc aacgagaagc cgctcgcggg
     1141 gcgcctcggc gtcgcgcaca cgcgctgggc gacgcacggc ggcgtcacgg aggcgaacgc
     1201 gcatccgcac ctgtccggcg atcgcgtcgc cgtcattcat aacggcatca tcgagaacta
     1261 cgagccgctc aagaacgagc tcatcgagaa aggctacgag ttcaagtccg agaccgacac
     1321 cgaggtcgcg gcgcatctga tccacgacta cttgaagcag ggccacgact tgctcgaggc
     1381 gctgcggctt gccgtcgccc gcttcgtcgg cgcctacgcg ctgctcgtgt tcgacgccaa
     1441 ggaccccgag cgtatcgtcg tcacgcgcat cgcgagcccg ctcgtcatcg gccttggcat
     1501 cggcgagaac ttcgtcgcga gcggcgtgcc ggcgctgttg cccgtcacgc agcggttcat
     1561 ctatctcgag cagggcgacg tcgccgagat cacgcgcgac gccgtgaaga tcttcgacgc
     1621 gcagggtcgg cccgtcacgc gcgccgtgca cgagacgcag tggaatcagg aggccgccga
     1681 gaaggggccg taccgccact tcatgctcaa ggagatctac gagcagccga gcgcgctcgc
     1741 cgacacgcta tacgggcgcg tcgtcaacaa gcgcgtcgta cccgaatcgc tcgggccgcg
     1801 cgccgcggag ctgctgccga aagtgcagaa catccacatc gtcgcgtgcg gcacgagcta
     1861 tcacgcgggc agcgtcggcc gctattggtt cgagtcgatc gccggcgtgc cctgccaagt
     1921 cgagatcgcg agcgagtacc gctatcgcaa cgtcgtcgtg ccgccgaata cgctgttcct
     1981 gacgctgtcg cagtccggcg agccggcgga cacgctcgcg gcgctgcgga tggcgaagac
     2041 cgcgggctac ttggcgtcgc tgacgatttg caacagcccg cacagctcga tggtccgcga
     2101 gtccgacctc gtcatgatga cgcaggccgg tcctgagatc ggcgtcgcga gcaccaaggc
     2161 gttcacgacg cagctcttgt cgttgctact ggtcacgctg atgctcggcc gtcaccgcgg
     2221 tatgagcgcc gcgcgcgaga gcgacttcgt gccgcacctg taccacgcgg ccgccgccgt
     2281 cgagcaggcg ctgcagatga acgagctcgt gcgattgctc gccgaggact tcgccgagaa
     2341 gcaccacgcg ttgttcttgg gacgcggccc gatgtggccg atcgcgatgg aaggcgcgct
     2401 gaagctcaag gaaatctcct acatccacgc cgaggcgtat gcggccggcg agctcaagca
     2461 tgggccgttg gcgctcatcg acgaggacat gcccgtcatc gtcgtggcgc cgaacaacga
     2521 gctgctcgac aagctgaaga gcaacatgca ggtcgtccgc gcgcgcggcg gtcagctgta
     2581 cgtgttcgca gaccgagaga cgggcctcga gtcc
//
LOCUS       DATLEE010000072         3412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3026203, whole genome shotgun sequence.
ACCESSION   DATLEE010000072 DATLEE010000000
VERSION     DATLEE010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3026203"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1585)
                     /locus_tag="VM692_01500"
     CDS             complement(134..1585)
                     /locus_tag="VM692_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HUQ50863.1"
                     /translation="MRSAASVFVHEGRVLLADRTLWIVALLFVGLVGYGLYNGAVETD
                     ARAAAIEAIRSADDAAEAARHVEFENIMAGTKLPDPFANPTDPSSMGGGYGARNAIMP
                     NLRMAPLAFGQADLLPSYYRVSNGSRIGFIYDTEIENPWSLLSGHFDLAFVVTYLVPL
                     LIFALSYNFLSGDREQGTLRMLLAQPVGLAGVVAGKLGVRLAVVLACAVLLPCVALLV
                     ARPELRSAAELAALGTWAALVSAYVLFWFALVLAVNAFGRSSAANALTLIGCWVVLVL
                     VAPVVMNLAVSLASPTPSRTELATRTRVVTAEALERYSDLFGTDYEYTADPALLVPRD
                     GKIEVAPRLRAFYLEDRDVDRDLDVLLEQFETRLAGQQALVGRYGFLSPAVSVYEGIT
                     ALAGTGLRRHQHFQKLIKDFHREWKAFFEPKILGGLAISESDLARFPRFEWQEIDAHE
                     ARSEVASRLLQVAVPALLLIALGAWRLRRYSVV"
     gene            complement(1590..2984)
                     /locus_tag="VM692_01505"
     CDS             complement(1590..2984)
                     /locus_tag="VM692_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HUQ50864.1"
                     /translation="MLRLIAAKDLLEYLRDGRLYLAGGVVLVLLFTALGVGLERHGEV
                     AAERVAAQGLDYDDWLAQPERHPHDAAHQGMHVFKPQSPLAILDPGIDPFVGSTMWLQ
                     AHRMSELKFRPAQDATGLQRFGELSPAWVLQVLGPLLIIVLGFNAFAGEREQGTLRQT
                     LSLGVSPVRLLWGKALALCACLALLLVPAAVVIGAAMSTANDFSIADTAARFAWLALG
                     YGLYLGIAAFGVLAVSAWAPSSRVALVTLLGVWIAGVTLVPRAASDFARSWYPSPSRL
                     EFNAQLDSAVGAAASQAWQQEFGVTTRWGTQVPLDRWGRALQMDDQASYPVFDANFER
                     LWGTFARQQALQEWLGLAVPLLAIRAYSMGISTTDFAHHRDFSVAAEQQRRMIQDLVS
                     EDLVEHADTRRGGHFDYRASAELWAKVPRFQYRPPSVMWSLRENLRSLAVLAVTFAAA
                     VALAHLAAVRRARS"
     gene            complement(2987..>3412)
                     /locus_tag="VM692_01510"
     CDS             complement(2987..>3412)
                     /locus_tag="VM692_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUQ50865.1"
                     /translation="NLRYFAALGTGKKASRSELLELLATVGLDEAAAVRRVSTYSKGM
                     RQKVGIAIAIAKQARAFLLDEPTSGLDPKAANEFSQLLRKARERGVAVLTTTHDLFHA
                     KQTGTRIGIMRAGKLVADLKGADLTDADLEALYLQHMGA"
BASE COUNT          626 a         1175 c         1141 g          470 t
ORIGIN      
        1 attgctactg aatcgcgtat ccacatcgat cccgacggtt cggatgtcac gatgccgccg
       61 cctacgcgcg acgagcgtcg cttgacggcg acgggcggcg cggcaaagca gtcggcgaac
      121 gcgcgctagg cgcttagacg acggagtagc gccgcagccg ccacgcgccg agcgcaatca
      181 gcagcagcgc cggcaccgcg acttgcagca agcgactcgc gacctcgctg cgcgcctcgt
      241 gcgcgtcgat ctcctgccat tcgaagcgcg ggaaacgcgc gagatcgctc tcgctgatcg
      301 caaggccgcc gaggatcttc ggctcgaaga acgccttcca ctcgcggtgg aaatccttga
      361 tgagcttttg aaagtgctgg tgacgccgca gacccgtgcc ggcgagcgcc gtgatgcctt
      421 cgtagacgct gacggcaggc gacaggaagc cgtagcggcc gacgagcgcc tgctgcccag
      481 cgagccgcgt ctcgaactgc tcgagcagca cgtcgaggtc tcggtccacg tcgcggtctt
      541 cgaggtaaaa ggcgcgcaga cgcggtgcga cctcgatctt gccgtcgcgc ggcacgagta
      601 gcgcaggatc cgccgtgtac tcgtaatcag tgccgaacag gtcgctgtac cgctcgagcg
      661 cctcggccgt cacgacgcgc gtgcgcgtcg cgagctccgt gcgcgagggc gtcgggctcg
      721 ccagcgagac ggcgagattc atgacgaccg gcgcgacgag cacgagcacg acccagcagc
      781 cgatcagcgt cagtgcattc gcggccgacg agcggccgaa cgcgttgacc gcgagcacga
      841 gtgcgaacca aaacaacaca taggcgctga cgagcgcggc ccacgtgccg agcgccgcga
      901 gctcggccgc cgagcgcagc tccggccgcg cgacgagcag cgcgacgcac ggcagcagca
      961 ctgcgcacgc gagcacgacg gcgagccgca cgccgagctt gccggcgacg acgccggcga
     1021 ggcccaccgg ctgcgcgagc agcatgcgca gcgtcccctg ctcgcgatcg ccggatagga
     1081 agttgtacga caacgcgaaa atcagcagcg gcacgagata cgtcacgacg aacgcgagat
     1141 cgaaatggcc gctcaggaga ctccacggat tctcgatctc cgtgtcgtag atgaagccga
     1201 tgcggctccc gttcgagacg cggtagtagc tcggcaatag gtccgcttga ccgaaagcga
     1261 gcggcgccat acgcaggttc ggcatgatgg cgttgcgtgc gccgtagccg ccgcccatcg
     1321 atgacgggtc ggtcggattc gcgaatgggt ccggcagctt cgtgccggcc atgatgttct
     1381 cgaactcaac gtggcgcgcc gcttcggctg cgtcgtcggc gctgcgaatc gcctcgatgg
     1441 ccgcggcgcg cgcgtcggtc tcgacggcgc cattgtagag gccgtaaccg acgagcccca
     1501 cgaacaacag cgcgacgatc cacagcgtcc ggtccgcgag caggacgcga ccttcgtgca
     1561 cgaacacgct cgcggccgaa cgcatcgcgt cagctcctcg cccgccgcac ggccgcgagg
     1621 tgcgcgagtg cgacggccgc ggcgaacgtc accgcgagca cagccaagct gcgcaagttt
     1681 tcgcgcaagg accacatgac cgacggcggc cggtactgga accgcggcac cttcgcccag
     1741 agctcggcag acgcgcgata gtcgaagtgc ccgccgcggc gcgtgtcggc gtgctcgacc
     1801 aagtcctcgc tgacgaggtc ttgaatcatg cgccgctgct gctcggccgc gaccgaaaaa
     1861 tcgcggtggt gcgcgaagtc cgtcgtcgaa atacccatcg agtaggcacg gatcgcaagc
     1921 agcggcaccg cgagaccgag ccactcctgc agcgcctgtt ggcgcgcgaa cgtgccccag
     1981 agccgctcga agttcgcatc gaacacggga tacgacgcct gatcgtccat ttgcagcgcg
     2041 cggccccagc ggtcgagcgg cacctgcgtt ccccagcgcg tcgtcacgcc gaactcttgc
     2101 tgccacgcct gcgacgccgc ggcaccgacg gccgagtcga gctgcgcgtt gaactcgagc
     2161 cgcgatgggc tcggatacca tgaacgcgcg aagtcggacg ccgcgcgcgg cacgagcgtg
     2221 acgccggcga tccacacacc gagcagcgtg acgagcgcga cgcgcgacga cggtgcccac
     2281 gccgacacgg ccagcacgcc gaacgccgcg atgccgaggt agagcccgta gccgagcgcg
     2341 agccaagcga aacgcgcagc cgtgtccgcg atcgaaaagt cgttcgccgt gctcatcgcc
     2401 gcgccgatca cgacggcggc cggcaccaag agcaacgcga gacaggcgca cagcgccagc
     2461 gctttgcccc agagcagacg cacgggcgaa acgccgaggc tcagcgtctg gcgcagcgtg
     2521 ccctgctctc gctcgccggc gaacgcattg aagccgagca cgatgatcag cagcggaccc
     2581 aggacttgca gcacccaagc cggcgaaagc tcgccgaagc gctgcaggcc cgtcgcgtcc
     2641 tgcgcgggcc tgaacttgag ctcgctcatg cggtgcgctt gcagccacat cgtcgagccg
     2701 acgaacggat cgatgccggg atcgagaata gccagcggcg attgcggctt gaacacgtgc
     2761 ataccctgat gcgcggcgtc gtgcggatgg cgctcgggtt gggcaagcca atcgtcgtag
     2821 tcgagcccct gcgcagcgac gcgctcggcc gcgacctccc cgtggcgctc gaggccgacg
     2881 ccgagcgcgg tgaatagcag cacgagcacg acgccgccgg cgaggtatag gcggccgtcg
     2941 cgcagatact cgagcaaatc tttcgcggcg atcaaacgca gcatcgtcac gcgcccatgt
     3001 gctgcagata cagcgcctcg agatcggcgt cggtcaggtc cgcgcctttg agatcggcaa
     3061 cgagcttgcc ggctctcatg atgccgatgc gcgtgccggt ctgcttggcg tgaaacaaat
     3121 cgtgcgtcgt cgtcagcacg gcgacgccgc gctcgcgcgc tttgcgcaag agctgcgaga
     3181 actcgttcgc ggccttgggg tcgaggcccg acgtcggctc gtcgagcaag aatgcgcgcg
     3241 cctgttttgc gatcgcaatc gcaatgccga ccttttgccg catccctttc gagtacgtcg
     3301 aaacgcggcg cacggccgcc gcctcgtcga gcccgaccgt ggcgagcagc tcgagcaact
     3361 cgctgcgcga tgccttcttg ccggtgccga gcgcagcaaa ataacgcaga tt
//
LOCUS       DATLEE010000073         7614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4558342, whole genome shotgun sequence.
ACCESSION   DATLEE010000073 DATLEE010000000
VERSION     DATLEE010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4558342"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VM692_01515"
     CDS             <1..819
                     /locus_tag="VM692_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUQ50866.1"
                     /translation="VHFLLIGVALFAVSAWRGESVSSGREEIVVTAEQVAQARAAAAV
                     LQGREPTEDEMAAIIEPTVRDEVLYREALALGLDENDDEVRRRLIEKMNYLTQDLADP
                     APPSEQALRDFYAANPATFTIPALVSFDQVFFSPGTRGDTLAAAAAAGLAAVRAGRAP
                     AEVGDRTPLRESYDDAPREQVAVLFGDELADALFGAAPGEWTGPFRSDFGLHVVRLRG
                     RSAARLPPYDEIAARVAEEYGAQRRREANERAYREMRARYDIVIEDPPAADAAQ"
     gene            816..1832
                     /locus_tag="VM692_01520"
     CDS             816..1832
                     /locus_tag="VM692_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HUQ50867.1"
                     /translation="MTRARRGIALVAAVSLSAPALAHRLSPAFFGLTETAPNVFDVQG
                     KVSISGGLAAVLEPQVPPGCAIASGGVRTSTLDDVRLQHATLECPDGLAGKPFAVKGL
                     AQTQTDVLLRVDYLDGSSSNQRLTPDTAAVTIPERPSAFEVVRTYTALGVEHILLGVD
                     HLLFVLALLLLVNGVGRLVATVTAFTLAHSVTLGAATLGFVHVPPAPVEAIIALSILF
                     LASELARRRGVAPGAADDSANLTARFPWVVAFSFGLLHGFGFAGALSEVGVPQQAVPL
                     ALLFFNVGVELGQLLFIAAVFGFAWLVKLAAIQVPPIWQRTVAYGIGSVAAFWVVERT
                     LAIF"
     gene            complement(2267..3091)
                     /locus_tag="VM692_01525"
     CDS             complement(2267..3091)
                     /locus_tag="VM692_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50868.1"
                     /translation="MAGALVAAPCAGVRAQSADATPFNWAYAPALGAGVYRLGDGTEA
                     QTYRGNFSLALRDAPASNEDRAGIRLLLPVALGVQNLDSEALPVDRPTNRVEHAGFLP
                     GVELEHRVGARWTLRTRAQLGAAEELEGTEQSARLAAVGLRGRLAFDSAPGKPALIAG
                     LLWAGFDPNVGERESLLRATTAVELDIRAARWRVRDSPMHWRPHVLKDWYYRSTPRLG
                     FGDSDAEPLDEEWQVGIAAAREDGFKIGFAKFDAVGVAYRFSDRSRGLRLYLNSVF"
     gene            complement(3234..3806)
                     /locus_tag="VM692_01530"
     CDS             complement(3234..3806)
                     /locus_tag="VM692_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HUQ50869.1"
                     /translation="MSKQPAPQEAAWPAGTLFTVGHSTLPIERFVAVLAAFGIERLAD
                     VRTVPRSRRNPQFNADALGASLRAAGIEYVPLGELGGLRKPRADSPNAGWRNESFRGY
                     ADYMQTEPFGAGLERLIELSRERRTAIMCAEAVPWRCHRSLVADALLARGIAVVEILS
                     ETSYRNHALTSFARVAGTTVTYPPAQASLL"
     gene            3887..5965
                     /locus_tag="VM692_01535"
     CDS             3887..5965
                     /locus_tag="VM692_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ50870.1"
                     /translation="MSRVTSALLALLCTAAAHAQAPNAEWRSITAERLRAPEAGDWLG
                     YRRTYDVQAFSPLREINRRSVERLRAVWAYTVRDNSRWVATPIVANGLIYIAEGSGRV
                     LALDAVTGELVWQHVRTYPQDIASSEAYPRHRGVAVYGDVVYFPTADSFLVALDARTG
                     SKLWEVRTGDYKTGEGHAHAPLVADGKVVVGNTGGDFAARGKFEAYDAANGARVWTFF
                     TVPRKGEPGFETWTENDAWPPLGAAAWNTASYDAELDLVYFSTGQPTPWSTAARGPGD
                     SLYSNTLLAVEADTGKLRWHFQLVPGDEWDRSAYEGMLVDLVIDGRQRKAVILTGKIG
                     WGVVLDRATGEFLHAFRTAYDNTITGWTPQGRPIFDPRAIPEAADVDSGKVFEICPHI
                     HGARNLQSPSFSPLTGYYYLGVNNSCMNAQVVAPVFRPGQGLTGVTYTASLAPGYDYV
                     GEFVAFDPVSGERAWVYRPESGAPMTASALATAGGLVFGGTADRQFFALHSETGKLLW
                     QTRLNGDVSGAPVSYTIGGKQYIAVASGGRAAPTTTLGRLVGVDVPQGTGVLWVFALP
                     DATPRPVPRPTRSVRPGRSLTDGVFTASQAAQGKALFAQECDACHDLENYTGSNFSAK
                     WGGGTLADVYQDFALAMPPANAGGLTPATYASIVAYFASESGFPTGTAELPADAAQLR
                     LIAIGAAPAP"
     gene            complement(6080..6463)
                     /locus_tag="VM692_01540"
     CDS             complement(6080..6463)
                     /locus_tag="VM692_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HUQ50871.1"
                     /translation="MNEGVNRDQMSFSELLAWAWSETPPVHRNRSNLILHLVAVPLFV
                     LGHLLLTAAFVVDWWVALDALSLIVVSLVLQAIGHRLEPQQVAAFTGPRDFVRRLYAE
                     QFCNFWRFLFAGQWLAHLSASSKRL"
     gene            complement(6511..>7614)
                     /gene="tcuB"
                     /locus_tag="VM692_01545"
     CDS             complement(6511..>7614)
                     /gene="tcuB"
                     /locus_tag="VM692_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HUQ50872.1"
                     /translation="RARRVMQATELTREGARVLSVCNACRYCEQYCPAFQAMEDRLAF
                     ATADLNYLANLCHGCGECLYACQYAPPHEFAINVPQTFAKLRVQSYEQYAWPAALGAA
                     FRHQGVMTALLLVGAMVALLLGATLALNGLALLEPGTSSDFYAVVPHGVMVAVFGGVG
                     LFVLLALAIGAARCARELKSMGAPPHGGGTGAALRDALTLNHLHVAGRDCVSGPELRT
                     PWRRWLHHATFYGFALCFASTCVATLYHFSGAPAPYAYTSLPVVLGTAGGIGLAIGTA
                     GLLLHGRRRDAALSDATSRNLDRAFIVLLLATALTGLLLLVLRHERAMGLLLVVHLGV
                     VLALFVTLPYGKLVHGVYRTIALRYYRRSHHEH"
BASE COUNT         1206 a         2620 c         2633 g         1155 t
ORIGIN      
        1 gtgcactttc tactgatagg cgtcgcgctg ttcgcggtct cggcttggcg cggtgagtcc
       61 gtgtcgtcgg ggcgcgagga gatcgtcgtc acggctgagc aggtcgcgca agcgcgcgcg
      121 gccgccgccg tgctgcaagg ccgcgagccg actgaagacg agatggcggc gatcatcgag
      181 ccgacggtgc gcgacgaggt cttgtatcgc gaagcgctcg cgctcgggct cgatgagaac
      241 gacgacgagg ttcgccggcg actcatcgag aagatgaact atttgacgca ggacctcgcg
      301 gatccggcgc cgccttccga gcaggcgttg cgcgacttct acgccgcgaa ccccgccacg
      361 ttcacgattc cggcgctcgt gagcttcgac caggtgttct tcagccccgg tactcgcggc
      421 gacacgctcg ctgcggccgc cgccgcgggg cttgcagctg tgcgcgccgg gcgcgcgccc
      481 gccgaagtcg gcgaccgcac gccgttgcgc gagtcgtacg acgatgcgcc gcgcgagcaa
      541 gtcgccgtgt tgttcggcga cgagctcgcg gatgcgttgt tcggtgccgc gcccggcgag
      601 tggacgggcc cgtttcgctc cgacttcggc ctgcacgtcg tccggttgcg cggccgcagc
      661 gctgcgcggt tgccgcctta cgacgagatc gccgcgcgcg tcgccgaaga gtacggcgcg
      721 cagcggcggc gcgaagccaa cgagcgtgcg tatcgcgaga tgcgcgcgcg ttacgacata
      781 gtgatcgaag atccgccggc ggcggacgct gcgcaatgac gcgcgcccgg cgtggcattg
      841 cgctcgtggc cgcggtctcg ttgtcggcgc ccgcgctcgc gcaccgcttg agccccgcgt
      901 tcttcggtct cacggagacc gcgccgaacg tgttcgacgt gcaggggaag gtgtcgatct
      961 cgggcggcct ggccgccgtg ctcgagccgc aggtgccccc cggctgcgcg atcgcgagcg
     1021 gtggcgtgcg cacgtcgacg ctcgacgacg tgcggctgca gcacgcgacg ctcgagtgcc
     1081 ccgatgggct cgccggcaag ccgttcgccg tcaaaggtct cgcgcagacg cagaccgacg
     1141 tgctgctgcg cgtcgactat ctcgacggca gctcgtccaa ccaacggctg acgcccgata
     1201 cggcggccgt cacgatcccg gagcggccga gcgcgttcga ggtcgtgcgg acgtacacgg
     1261 cgctcggcgt cgagcacatc ctgctcggcg tcgaccactt gctgttcgtg ctcgcgctgc
     1321 tgctgctcgt gaacggcgtc ggccggctcg tggcgacggt gacggcgttc acgcttgcgc
     1381 acagcgtcac gctcggcgcc gcgacgctgg gcttcgtgca cgtgccgccg gcgcccgtcg
     1441 aagcgatcat tgcgctcagc attctgtttc tcgcctctga gctcgcgcgc cgccgcgggg
     1501 tcgcgcccgg cgcggccgac gactctgcga atctgacggc gcgcttcccg tgggtcgtcg
     1561 cgttctcgtt cggcttgctg cacggcttcg gctttgccgg tgcgctcagc gaggtcggcg
     1621 tgccgcagca agccgtgccg ctcgcgctgc tgttcttcaa cgtcggcgtc gagctcggcc
     1681 aactgctgtt tatcgcggcg gtgttcggct tcgcgtggct cgtgaagctg gcggcgatcc
     1741 aagtcccgcc gatctggcag cggaccgtag cgtacggcat cggctccgtc gccgcgttct
     1801 gggtcgtaga gcgcacgctc gcgatcttct agctgtcgcc gcgttgcgac gctttcgagc
     1861 gctcacccaa gcgtctcgca cagcttccaa aaacaagaaa cgccgcgcgc gacgagaagg
     1921 cgcttccgct gtcgttcgtg cacatggcac gcaacttgct cctccgctat cgggcggcaa
     1981 gaaaaagggg acgccgcctt ctcggggagg tacccatgtt ttcgcgcgag gcgcagccgc
     2041 ctggctgccg ttgctagttg tcgtgctcgg ttgcaagcgt gcggtgatgg cggtggcggc
     2101 gacggagccc ccagtaaccc ccccgttaca ggcccgccgc caccgccagc tcccacggct
     2161 ctgagtttca cgagcccgca gagctacacg gtcggcatgg ccatcaccgc acgggcacgg
     2221 tcagcagcta tagcgtcgtg cccgcgctgc cgagcagcta gcgagctcag aacacggagt
     2281 tcagatacag gcgcaagcca cggctgcggt cggagaagcg gtaagcgact ccgaccgcgt
     2341 cgaacttcgc gaacccgatc ttgaagccgt cttcgcgcgc ggccgcgatg ccgacctgcc
     2401 attcctcgtc gagcggctcg gcgtcgctgt cgccaaagcc gagccgcggc gttgagcggt
     2461 aataccagtc cttcaagaca tgcggccgcc aatgcatcgg gctatcgcgc acgcgccagc
     2521 gtgcggcgcg tatgtcgagc tcgactgccg tcgtcgcgcg tagcaacgac tcgcgttcgc
     2581 cgacgttcgg gtcgaaaccc gcccacaaca gccccgcgat caaggccggc ttgcccggcg
     2641 ccgaatcgaa cgcgagccgg ccgcgcaatc cgacggccgc aagtcgcgcc gactgctcgg
     2701 tgccttcgag ctcctcggcc gcgccgagct gcgcgcgcgt gcgcaacgtc cagcgcgcgc
     2761 cgacgcggtg ctcgagctcg acgcccggta gaaagccggc atgctcgacg cggttggtcg
     2821 gccggtcgac gggcaacgcc tcgctgtcga gattctgcac gccgagcgcc acgggcagca
     2881 gcagcctgat gcccgcgcgg tcttcgttgc tggccggcgc gtcgcgcagc gcgagcgaga
     2941 aattgccgcg atacgtctgc gcttcggtgc cgtcgccgag ccgataaacg ccggcgccga
     3001 gagcgggcgc gtacgcccaa ttgaacggag tcgcgtcggc gctttgcgcg cggacgcccg
     3061 cgcagggcgc ggcgacgagt gcgccggcca cgcacagtag cgagagcgat ctccggatat
     3121 ttgcgagcat ccgttccttg gacgacgtcg agcggccaat ccatggccgt gggcgcgcgt
     3181 gcggccgaag cgcagcaagt tccgcgccaa agacgcccgt cggcgcgtgg tagttacagc
     3241 aggcttgctt gcgcgggcgg gtaggtgacg gtcgtgccgg ccacgcgcgc gaacgacgtc
     3301 agggcatggt tgcggtagct ggtctcgctc aagatctcga cgacggcgat accgcgcgca
     3361 agcagcgcat ctgcgacgag cgagcgatga cagcgccacg gcacggcctc ggcgcacatg
     3421 atggccgtgc gccgctcgcg ggacagctcg atgaggcgct cgaggcccgc gccgaacggc
     3481 tcggtctgca tgtagtccgc gtagccgcga aagctctcgt tgcgccagcc cgcattcggc
     3541 gaatcggctc gcggcttccg cagcccgccg agctcgccga gcggcacgta ctcgatgccg
     3601 gcggcacgca gtgacgcgcc gagcgcgtcg gcgttgaact gtggattgcg ccgcgagcgc
     3661 ggcacggtgc gcacgtcggc caagcgctcg ataccgaagg ccgccagcac ggcaacgaaa
     3721 cgctcgatcg gcaacgtcga atggccgacc gtgaataggg taccggccgg ccacgccgcc
     3781 tcctgcggcg cgggttgttt gctcacggcg cctccttggc accgcacatt tgagagtgcg
     3841 tcgcgaacca aaataccatg ggcgccggga accgccgcga gactctatga gccgagtgac
     3901 ttccgctctg ttggccctgc tctgcacggc cgccgcgcac gcgcaggcgc cgaacgcgga
     3961 atggcgatcg atcacggccg agcggctccg cgcgcccgaa gccggcgatt ggctcggcta
     4021 ccggcggacg tacgacgtcc aagcgttcag cccgttgcgc gagatcaacc gccgcagcgt
     4081 cgagcggctg cgagccgtct gggcctacac ggtgcgcgac aacagccgct gggtcgccac
     4141 gccgatcgtc gcgaacgggc tcatttacat agcggagggc agcggccgcg tgctggcgct
     4201 cgacgccgtc acgggcgagc tcgtctggca gcacgtgcgc acgtacccgc aggacatcgc
     4261 ctcgtcggaa gcctacccgc ggcaccgcgg cgtcgccgtc tacggcgacg tcgtctattt
     4321 cccgacggcc gacagcttct tggtcgcgct cgatgcgcgc acgggctcga agctctggga
     4381 agtgcgcacc ggcgactaca agaccggcga gggccacgcg cacgcgccgc tcgtcgccga
     4441 cggcaaggtg gtcgtcggca ataccggcgg cgacttcgcg gcgcgcggca agttcgaagc
     4501 ttacgacgcc gcgaatggcg cgcgcgtctg gacgttcttc acggtgccgc gcaagggcga
     4561 gccgggcttc gagacctgga ccgagaacga cgcctggccg ccgctcggcg ccgcggcgtg
     4621 gaacaccgcg agctacgacg ccgagctcga tctcgtgtat ttctcgacgg ggcaaccgac
     4681 gccgtggagc acggccgccc gcggccccgg cgattcgctg tactcgaaca cgctgctggc
     4741 cgtcgaagcc gacaccggca agttgcgctg gcactttcag ctcgtgcccg gcgacgagtg
     4801 ggaccgttcg gcctacgagg gcatgctcgt ggatctcgtg atcgacggcc gacagcgcaa
     4861 ggccgtgatc ttgacgggca agatcggctg gggcgtcgtc ctcgatcgcg ccacgggcga
     4921 gttcttgcac gcgttccgca cggcctacga caacaccatc acgggctgga cgccgcaggg
     4981 ccggccgatc ttcgatccgc gcgcgatccc agaggctgcc gacgtcgact cgggcaaagt
     5041 gttcgagatc tgtccgcaca tacacggcgc gcgcaatctg caatcgccga gcttcagccc
     5101 gctgacgggc tactactatc tcggcgtcaa caactcgtgc atgaacgcgc aagttgtcgc
     5161 gccggtgttc cggccggggc aggggctcac gggcgtgacc tatacggcgt cgctggcacc
     5221 gggctacgac tacgtcggcg agttcgtcgc gttcgatccc gtcagcggcg aacgggcgtg
     5281 ggtctatcgg cccgaaagcg gcgcgccgat gacggcgtcg gcgctcgcga cggcgggcgg
     5341 gctcgtcttc ggcggcacgg cggaccggca gttcttcgcg ctgcattccg agaccggcaa
     5401 gttgctgtgg cagacgcgct tgaacggcga cgtttcgggc gcgcccgtca gttacacgat
     5461 cggcggcaag caatacatcg ccgttgcgtc gggtggccgc gcggcgccga cgacgacgct
     5521 cgggcggctc gtcggcgtcg acgtgccgca aggcacgggc gtgttatggg tgttcgcgct
     5581 gcccgatgcg acgccgcggc cggtgccgcg cccgacgcgc agcgtccgcc cggggcgctc
     5641 gttgaccgac ggcgtgttca cggccagcca agcggcgcag ggcaaggcgc tgttcgcaca
     5701 agagtgcgac gcgtgccacg acctcgagaa ctacacgggt agcaatttct ccgcgaagtg
     5761 gggcggcggc acgctcgccg acgtctatca ggacttcgcg ctcgcgatgc cgccggcgaa
     5821 tgccggcggt ctgacaccgg cgacgtacgc gagcatcgtc gcgtacttcg cgagcgagag
     5881 cggcttcccg accggcaccg cggagctgcc cgccgacgcg gcgcaattac gcttgatagc
     5941 gatcggtgcg gcgccggcgc cctaaccgga cactgcagag ttcgccgcgc ttcctcgtgc
     6001 accaacgagg tgcagaatcc gccacgtggc gggtttcgat cgtgagggtc acgctttcgc
     6061 gcgatcatga cgagcactgt catagtcgtt tgctcgaggc gctcagatgg gccagccact
     6121 gaccggcaaa caggaagcgc cagaagttgc agaactgctc cgcataaagc cgtcgcacga
     6181 aatcgcgcgg ccccgtgaac gcggcgactt gctgcggctc gaggcggtgg ccgatcgcct
     6241 ggagcacgag cgagacgacg atgagcgaca gcgcgtcgag cgctacccac cagtcgacga
     6301 cgaacgccgc ggtcagcagc aagtgcccaa gcacgaacag cggcacggcg accaagtgaa
     6361 ggatcagatt cgatcggttc ctgtgcaccg gcggcgtctc gctccacgcc catgcgagca
     6421 gctcgctgaa cgacatctga tcgcggttca cgccttcatt cataggacct ccgcagtgcc
     6481 cgagatgata ggcggcaact gcgcgcgcac tcaatgttcg tgatgcgagc ggcggtaata
     6541 ccgcaatgcg atggttcggt agacgccgtg cacgagcttg ccgtaaggca aagtcacgaa
     6601 cagcgcgagc acgacgccta agtgcacgac gagcagcagt cccattgcgc gctcgtggcg
     6661 cagcacgagc aatagcaggc ccgtgagcgc cgtcgccagc agcagcacga tgaacgcgcg
     6721 atcgaggttg cgactcgtcg catcggacaa cgccgcgtcg cggcggcgtc cgtgcagcag
     6781 caacccggcc gtgccgatag cgaggccgat gccgccggcg gtgccgagaa caaccggcag
     6841 actcgtgtag gcgtacggcg ccggcgcgcc gctgaagtga taaagcgtcg cgacgcaggt
     6901 cgacgcgaag cacagcgcga agccgtagaa cgtcgcgtga tgcagccagc gccgccacgg
     6961 cgtgcggagc tcggggccgc tgacacagtc gcggcccgcg acgtgcagat gattcaacgt
     7021 caacgcgtcg cgcagcgccg cgcctgttcc accgccgtgc ggcggcgccc ccatgctctt
     7081 gagctcacgc gcgcagcgcg ccgcgccgat cgcgagcgcg agcaacacga acagcccgac
     7141 gccgccgaac acggcgacca tgacgccgtg cggcacgacg gcatagaagt ccgagctcgt
     7201 gcctggctcg agcagcgcga ggccattgag cgccagcgtc gcgccgagca gcaacgcaac
     7261 cattgcgccg acgagcagca gcgccgtcat gacgccttga tgacggaacg ccgcgccgag
     7321 cgccgctggc caggcgtact gctcgtacga ttgcactcgc aacttcgcga acgtctgcgg
     7381 cacgttgatg gcgaactcgt gcggcggcgc gtattggcac gcgtacaagc attcgccgca
     7441 gccgtgacag aggttcgcga ggtagttcaa gtccgccgtc gcaaacgcga ggcgatcctc
     7501 cattgcttgg aacgcggggc agtactgctc gcaatagcgg cacgcgttgc agaccgacag
     7561 cacccgcgcg ccttcgcgcg tcagctcagt ggcttgcatg acgcgccgcg ccct
//
LOCUS       DATLEE010000074         3423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8243330, whole genome shotgun sequence.
ACCESSION   DATLEE010000074 DATLEE010000000
VERSION     DATLEE010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8243330"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2261
                     /locus_tag="VM692_01550"
     CDS             <1..2261
                     /locus_tag="VM692_01550"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUQ50873.1"
                     /translation="QPGRSARNVRVGGEPLAGTRALRDGDVVAFDRARLECRVAGSAL
                     TIRIDWIVTAGDTAPPDLDVVARGSRGAEIAITPIAFKPATTAEQASRRVGKGTIGVA
                     TAGVLLAAVAWFAFTAKSVALDIQPPPTAMSLPSTLLKLKLGDRFLLRSGSHRVAAEL
                     PGYYPLDTNVEVGPLSRQTIELNLTKLPGLVTITTEPEVAAQVSVDGMPLGMTPLVDA
                     ELTPGVHRLEFNAPRHLAEVRELEVGGGGERQSLAATLTPAYAVVSLRTDPPGATVLV
                     DGAETGVTPAEIEIDSGEHELEVRLAGYNAWSDKILVAANQPQQLPDVKLALADGRVE
                     LASAPSEASVSVDGEFRGRTPLSLRLAPGRAHRVTLTKPGYETAARELSVGADSGRRL
                     QVELTPQLGQIEVVSTPPNAEVWVGGERRGTTPTTLELTAVSQDIELRLAGYAQERAD
                     LTPRPGYPQKLERTLVALNEASGGGFAQSIRTTAKQELKLVPAGQFTMGSSRREVGRR
                     SNEMLRPVRVTRAFYLGVNEVTNAEFRAFKPDHDSGEFGGESLNGDDQPVANVTWDDA
                     VEYLNWLSIKDGLQPVYEAQANGWAAVRPLRNGYRLPTEAEWEWAARFAGQEAALHYP
                     WGAELPPPDRSGNYADVTASRILPSVLVTYNDGASVAAPVGRYAANSYGIFDLGGNVA
                     EWVQDLYAPDVVETTERVDDPLGPDTGRLHVVRGASWRSATVTDLRLAARGSGTEGRD
                     DVGFRIARNLQK"
     gene            2265..2519
                     /locus_tag="VM692_01555"
     CDS             2265..2519
                     /locus_tag="VM692_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50874.1"
                     /translation="MNISGKIPTALLALLCGAVTLAQEGASPAPPATPPRAPAPAPAG
                     SGDKADTAPAPDQRPEVKDDEFIPTEELQPDAAVTFPVDI"
     gene            2527..>3423
                     /locus_tag="VM692_01560"
     CDS             2527..>3423
                     /locus_tag="VM692_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50875.1"
                     /translation="MAALAIDINDVGLVVASDSALLATEPGFARIDRGKIVTGDAAKA
                     GARLQPRQTSNRFWSALSLEPGTAGADLGKSAAELAYAQLDSLWRKVGNGATDVVLVV
                     PGAYRTEQLGVLLGLAQECGMPVRALVDTAAAASVRPYAGRQLMYLDAGLHRASVALL
                     DQDEDAHVRAEHALNQGLSGITDAFARRIADLFVRSTRFDPFAHAETEQQLYDRLSQW
                     LSELQRDGRAELKLDYRNDEVRVEAERDAVLGVAHGFCRALVQLVAQHRERGKGLVVQ
                     VSDRLAAVPGFLSELARLDDAQA"
BASE COUNT          533 a         1175 c         1214 g          501 t
ORIGIN      
        1 tgcagcccgg ccgcagcgcg cgcaacgttc gcgtcggcgg cgagccgctc gcgggtacgc
       61 gtgcattgcg cgacggcgac gtcgtcgcgt tcgatcgcgc gcggctcgag tgccgtgtcg
      121 caggttcagc gctgacgatt cgcatcgact ggatcgtgac ggccggcgac acggcgccgc
      181 cggatctcga cgtcgtcgcg cgcggcagtc gcggagccga gatcgccatc acgccgatcg
      241 cgttcaagcc cgcgacgacg gccgagcaag cgagcaggcg cgtcggcaaa ggaacgatcg
      301 gcgtcgcgac ggcgggcgtc ctcttggccg ccgtggcctg gtttgcgttc acggcgaaat
      361 cggtcgcgct cgacattcag ccgccgccga cggccatgag ccttccgagc acgctgctga
      421 agctgaagct cggcgatcgc tttctattgc gttctggctc gcatcgcgtc gcggccgagc
      481 tgccgggtta ctacccgctc gacacgaacg tcgaggtcgg cccgctgtcg cggcagacga
      541 tcgagctcaa cctgacgaag ctgccggggc tcgtgacgat cacgacggag cccgaagtgg
      601 ccgcgcaggt ttccgtcgac ggcatgccgc tcggaatgac gccgctcgtc gacgccgagc
      661 tgacgccggg cgtgcatcgg ctcgagttca acgcgccgcg gcatctcgcc gaggtgcgcg
      721 agctcgaggt cggaggcggc ggcgaacggc agagcttggc ggcgacactg acgcccgcct
      781 acgcggtcgt gtcgctgcgc accgatccgc ccggtgcgac ggtgctcgtc gacggcgccg
      841 aaaccggcgt gacaccggcc gagatcgaga tcgactccgg cgagcacgag ctcgaggtgc
      901 gcttggcggg ctacaacgcc tggtccgaca agattctcgt cgccgcgaac cagccgcagc
      961 agctacccga cgtgaagctc gcgctcgccg acgggcgcgt cgagcttgct agcgcgccga
     1021 gcgaggcgag cgtcagcgtc gacggcgagt tccgcggccg cacgccgctg tcgctgcggt
     1081 tggcgccggg tcgcgcgcat cgcgtcacgc tgacgaaacc gggatatgag acggccgcac
     1141 gcgagctatc ggtcggagcc gacagcggcc ggcgtttgca ggtcgagctg acgccgcagc
     1201 tcggccagat cgaggtcgtc agcaccccgc cgaacgccga agtttgggtc ggcggcgagc
     1261 ggcgcggcac cacgccgacg acgctcgagc tgacggccgt gagtcaggac atcgagcttc
     1321 ggctcgcggg ctacgcgcag gagcgcgccg acttgacgcc gcggccgggg tatccgcaga
     1381 agctcgagcg aacgctcgtg gcgctgaacg aagcgtcggg aggcggcttc gcgcagtcga
     1441 tccgaacgac ggccaagcaa gagctgaagc tcgtgccggc ggggcagttc acgatgggct
     1501 cgtcgcgacg tgaagtcggc cggcgctcga acgagatgct gcgccccgtg cgcgtgacgc
     1561 gcgcgttcta ccttggcgtc aacgaagtca cgaacgccga gtttcgcgcg ttcaagcccg
     1621 atcacgactc cggcgagttc ggcggcgaat ccctgaacgg cgacgaccag cccgtcgcca
     1681 atgtcacttg ggacgacgcc gtggagtatc tgaactggct cagcatcaag gacgggttgc
     1741 agcccgttta cgaggcgcaa gcgaacggct gggcggccgt gcgtcccctg cgcaacgggt
     1801 atcgtttgcc gaccgaagcc gagtgggaat gggccgcgcg gttcgcgggc caagaggcgg
     1861 cgctgcacta tccatggggc gccgagctgc ccccgccgga ccgctccggc aactacgccg
     1921 acgtgacggc ttcgcgcatc ctgccgagcg tcctcgtgac ctacaacgac ggcgcgtccg
     1981 tcgcggcgcc ggttgggcgc tatgcagcca atagctacgg catcttcgat ctcggcggca
     2041 acgtcgccga gtgggtccag gacctctacg cccccgacgt ggtcgaaacg accgagcgcg
     2101 tcgacgatcc gctcggcccg gacactggac gcttgcacgt cgtccgcggc gcgagctggc
     2161 gcagcgcgac ggtgaccgac ctgcgactcg ccgctcgcgg ctccggaacc gaagggcgcg
     2221 acgacgtggg ctttcgcatc gctcgcaacc tacagaagta gcgtatgaac atttcaggaa
     2281 agattccgac cgcgctgttg gcgctgctgt gtggcgccgt aacgctcgcg caggaaggcg
     2341 cctcgccggc gccgcccgcg accccgccgc gcgcaccggc tccggcgcct gccggcagcg
     2401 gcgacaaggc cgacaccgca ccggcgccgg accagcgccc cgaggtcaag gacgacgagt
     2461 tcattcccac ggaagagttg cagccggacg cggccgtgac cttccccgtc gacatctgaa
     2521 cggcgcatgg ctgcactggc gatcgacatc aatgacgtag gcctcgtcgt cgcgagcgac
     2581 tcggcgttgc tcgcgaccga gccggggttt gcgcgcatcg accgcggcaa gatcgtcacg
     2641 ggcgatgccg cgaaggccgg cgcgcgcctg cagccgcgtc aaaccagcaa ccgtttctgg
     2701 agcgctctga gcttggagcc cggcaccgcg ggcgccgact tgggaaagag cgcggccgag
     2761 ctcgcatacg cgcagctcga ttcgctgtgg cgcaaggtcg gcaacggcgc cacggacgtc
     2821 gtgctcgtcg tgcccggcgc gtatcgcacg gagcagctcg gcgtgctgct cgggctcgct
     2881 caggaatgcg gcatgcccgt gcgcgcgctc gtcgacacgg ccgcggccgc cagcgttcgg
     2941 ccgtatgcgg gccggcagct catgtatctc gacgccggct tgcatcgcgc gtcggtcgcg
     3001 ctgctcgatc aggatgagga cgcgcacgtc cgcgcagagc acgcgttgaa tcaagggctc
     3061 agcggcatca ccgatgcttt cgcgcgccgc attgccgatc tgttcgtgcg ttcgacgcgg
     3121 ttcgatccgt tcgcgcacgc cgagacggag cagcaactct acgaccgctt gtcgcaatgg
     3181 ctttccgagc tgcagcgcga cggccgcgcg gagctcaagc tcgactaccg taacgacgag
     3241 gttcgcgtcg aggccgagcg cgatgccgtg ctcggcgtcg ctcacggttt ctgccgcgcg
     3301 ctcgtgcagc tggtcgcgca gcaccgcgag cgcggtaagg gcctcgtcgt gcaggtgtcg
     3361 gaccggctgg cggcagtgcc gggattcttg agcgagcttg cgaggctcga cgacgcgcaa
     3421 gcc
//
LOCUS       DATLEE010000075         4247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9734881, whole genome shotgun sequence.
ACCESSION   DATLEE010000075 DATLEE010000000
VERSION     DATLEE010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9734881"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VM692_01565"
     CDS             <1..218
                     /locus_tag="VM692_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUQ50876.1"
                     /translation="LRAWEMTANGRIRMGDVIEAVDGRPVKNFEELISVLDSHEFGDR
                     ITLTVRRDEQRVDVPITLASADAARTR"
     gene            301..990
                     /locus_tag="VM692_01570"
     CDS             301..990
                     /locus_tag="VM692_01570"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUQ50877.1"
                     /translation="MNERVELSVPSAAPDSIPDAAIPDAEVVARVLTGDSALFELLMR
                     RYNRLLFRLARGIVRDDDEARDVVQAAYVRAFYHLSQFRGPANFKAWLARIALNEALG
                     RVRRAPATVEAEQQHVLALPDLVTVEPEHAASGRDVLRILQVAIDRLPEEFRQVFMLR
                     GVEQLSIAETAELLEIKPATVKTRFHRARRLLQELLQRELDDVVRETFPLGGQRCDAI
                     VGTVLARIRFQ"
     gene            1024..2346
                     /locus_tag="VM692_01575"
     CDS             1024..2346
                     /locus_tag="VM692_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50878.1"
                     /translation="MLRRLRVGTFAASLLALPFGATSADDLPLTANAALWQNAAQQVS
                     RGRNTFRFDTFGDQEFWGGALRLHEAIAGAANGGVGPGLSPKAALGLGLKVDAKALPG
                     PLVARLKAQRVNLDDPAVTLALLKLNAVVGVRVFTDRAGKIETVGITCALCHSTVDDS
                     LAPGIGGRLDGWANRDLNVGEIIALAPSVAPFSQLLGVDDATVRKVFKSWGPGKFDAE
                     LLLDGKAFRPDGRSAATLLPPAFGLAGVNLHTWTGWGSVTHWNAFVAVLEMQGKGTLY
                     DPRLDDASKFPIAAANGFGHVRREPDLVTPKLGALQFYQLAIPAPTPPDGSFDRRAAE
                     RGKALFAADGKAGCASCHVPPLYTEPGFNLHKPEEIGIDAFQAQRGPEDGYRTSPLRG
                     LWTHTNGGFYHDGRFPTLRAVVDHYDSFQSLGLNDSEKRELVEFLKSL"
     gene            complement(2374..2979)
                     /locus_tag="VM692_01580"
     CDS             complement(2374..2979)
                     /locus_tag="VM692_01580"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HUQ50879.1"
                     /translation="MRQAHINLSLLVVCAAAAVISAQPTPQPPPPDASSPPEAHERAN
                     VSDAQLGLEVQSRLYQALDISNLSALVRYGVATLNGRVHSEEDKQRAQQMALDVQGVE
                     SVVNELLVETPVVVALATQAQEIVEREDSDLETEVTNRLRLDPVLGSRNIRVDTDDVG
                     NTLTLTGTVSTQDEKEQAGRIAVNSFPAGRVRNQIEVQQRL"
     gene            complement(3053..>4247)
                     /locus_tag="VM692_01585"
     CDS             complement(3053..>4247)
                     /locus_tag="VM692_01585"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HUQ50880.1"
                     /translation="EHGAAPAAARLAALLSERDLLRAGARDSDIRTRLETVQRGGGDR
                     GVLERVRRAERAYLREVGAGDDTGTDTHDVGWLLAFAYPDRVGKRRPGDAGRYQLANG
                     RGASFKGPEAIAREELIVAVELDDREREAQIQLAAPLARSDLLEHFASEIVTRDEVAW
                     DGRAEAVIARRAVRFGELVLDEKPLPELPAEATSAALLDGLQSLGLDSLPWDDDAHTF
                     AARNEFVRKLGRGDLADWPDFSPAALAADLAWLEPYLDGITRRSHFARIPLLAALRAR
                     LSAAQARALDELAPTHITLPTGTRAAVDYRDDNAPCASMRMQEVFGLAATPRIGGGQV
                     PITFKLLSPAHRPLQVTRDLASFWKNAYIEVRKDMRGRYPRHYWPENPLEAEPTRRAK
                     PRPPR"
BASE COUNT          642 a         1435 c         1460 g          710 t
ORIGIN      
        1 ggctgcgcgc gtgggagatg acggccaacg gccgcatccg gatgggcgac gtcatcgagg
       61 ccgtcgacgg ccggccggtc aaaaatttcg aggaactcat cagcgtgctc gactcgcacg
      121 agtttggcga tcgcatcacg ctgacggtcc gccgtgacga gcagcgcgtc gacgtgccga
      181 tcacgctcgc ctcggccgac gctgcgcgga cgcgctgaac atcatccagt gaaccgttag
      241 tcgcggcgcg gccacccacg gccgtgggaa ccttttcgag gccgcgagca tcttgtgccc
      301 atgaacgagc gagtggagct gagcgttcct agcgcggcac cggactctat cccggacgct
      361 gcgatccccg acgccgaagt cgttgcgcgc gtgctgacgg gcgactcggc gctcttcgag
      421 ctcttgatgc ggcgctacaa ccgtctgttg ttccgtctgg cccgcggaat cgtgcgcgac
      481 gacgacgagg cgcgcgacgt cgtgcaggcc gcttacgtgc gcgccttcta ccacctcagt
      541 cagtttcgcg gccccgccaa cttcaaggca tggctcgccc gtattgcgct caacgaggcg
      601 ctcggccgcg tgcggcgcgc gccggccacc gtcgaggccg agcaacagca cgtgctcgcg
      661 ctgccggacc tcgtcaccgt cgagccggag cacgccgctt cgggtcgcga cgtgctgcgc
      721 atcctgcaag tggccatcga tcgactgccg gaggagtttc ggcaggtttt catgttgcgc
      781 ggcgtcgagc agctcagcat cgccgagacg gccgagctgc tcgaaatcaa accggccacc
      841 gtgaagacgc gttttcatcg agcgcgccgc ctgctccagg agttgctgca gcgcgagctc
      901 gacgacgtcg tgcgcgagac gttcccgctc ggcgggcaac gctgtgacgc gatcgtcggg
      961 accgtcctcg ctcgtatcag gtttcagtaa ccgtccaagg aaggagaacg tgtcatgacc
     1021 tcgatgcttc gtcggcttag ggtagggaca ttcgccgcgt cgttgctcgc tttgccgttc
     1081 ggtgcaacga gcgccgacga cctgccgctg accgccaatg cggcgctctg gcagaacgcc
     1141 gcgcaacaag tctcacgcgg ccgcaacacg tttcgcttcg ataccttcgg cgaccaagaa
     1201 ttctggggcg gggcgttgcg gttgcatgag gcgatcgcgg gtgccgccaa tggcggcgtc
     1261 ggccccggct tgagcccgaa agccgcgctg ggtctcgggc tcaaggtcga cgccaaagcg
     1321 ttgccgggtc cgctcgttgc gaggctcaaa gcgcagcgcg tgaacctcga cgatccggcc
     1381 gtcacgctgg cgctgttgaa gctcaacgcg gtcgttggag tgcgcgtatt cacggatcgg
     1441 gccggcaaga tcgagacggt cggtatcacg tgtgcgctct gccatagcac cgtcgacgat
     1501 tcgttggcgc ccggaatcgg cgggcggctc gacggctggg ccaatcgcga tctcaacgtc
     1561 ggcgagatca tcgccctcgc gccgagcgtc gcaccgttct cgcagttact cggcgtcgac
     1621 gacgcgaccg tgcgcaaggt cttcaagagc tggggccccg gcaagttcga cgccgagctg
     1681 ctgctcgacg gcaaggcgtt tcggcccgac ggccgcagcg cggcaacgct gttgccgccg
     1741 gcgttcggct tggccggcgt caacttacat acgtggaccg gctggggctc cgtcacgcat
     1801 tggaatgcgt tcgtcgccgt gctcgagatg cagggtaagg gcacgctgta cgacccgcgg
     1861 ctcgatgacg cgagcaagtt tccgattgcc gcggccaacg gcttcggcca cgtgcgccgc
     1921 gagcctgacc tcgtgacgcc gaagctcggc gcgctgcagt tctatcagct cgccattcct
     1981 gcgccgacgc cgcccgacgg cagcttcgat cggcgggcgg ccgagcgcgg caaggcactg
     2041 ttcgcggccg acggcaaagc gggttgcgcg agttgtcacg tgccgccgct ctacacggag
     2101 cccggcttca acctacacaa gcccgaggag atcggcatcg acgcgtttca agcgcagcgc
     2161 ggccccgagg acggctaccg aacgtcgccg cttcgcggac tctggacgca cacgaacggc
     2221 gggttctatc acgacggccg gttcccgaca ctgcgtgccg tcgtcgacca ctacgactcc
     2281 tttcagagct tgggcctgaa tgacagcgag aagcgcgagc tcgtcgagtt cctgaagtcg
     2341 ttgtagcggc gcagcggcta tggcgcgccc gcgctatagc cgctgttgta cctcgatctg
     2401 gttacggacg cggccggccg gaaagctgtt gacggcgatg cgtcccgcct gttccttctc
     2461 gtcctgggtc gagacggtac ccgtcagcgt cagagtattg cccacgtcgt cggtgtccac
     2521 gcggatgttg cgcgagccga gcacgggatc caaacgcagc cgattcgtga cttccgtctc
     2581 gaggtccgag tcctcgcgtt cgacgatctc ttgcgcttgc gtagcgagtg cgactacgac
     2641 cggcgtctcg acgagcagct cgttcacgac gctctcgact ccttggacat cgagcgccat
     2701 ctgttgcgct cgttgcttat cctcttccga gtgaacgcgg ccgttcagcg tcgcgacgcc
     2761 gtaccggacg agggcgctca agttactgat gtcgagtgct tgatagaggc gactttggac
     2821 ctcgaggccg agctgagcat cgctgacgtt cgcgcgctcg tgcgcctccg gcggcgacga
     2881 agcgtccggc ggtgggggtt gcggcgtcgg ctgggctgaa atgacggccg cggccgcaca
     2941 aaccacgagc aacgacagat tgatgtgggc ttggcgcatg tctactctcc ctgtacatag
     3001 gccccagcgc tgcaaatcgc gtgcccgcgt gcccccaccc gcgggccgag cgtcagcgcg
     3061 gcggccgggg cttcgcgcgc cgcgtcggct cggcttcgag cgggttctcg ggccagtagt
     3121 ggcgcggata gcgcccacgc atgtccttgc gcacttcgat gtaagcgttt ttccaaaaac
     3181 tcgcgaggtc gcgcgtgact tgcaacggcc ggtgggccgg cgacaacaac ttgaacgtga
     3241 tcggcacctg accgccgccg atgcgcggcg tcgccgccaa accgaagacc tcctgcatgc
     3301 gcatcgacgc gcagggcgcg ttgtcgtcgc gatagtcgac ggccgcgcgc gtgcccgtcg
     3361 gcaacgtgat gtgcgtcggt gcgagctcgt cgagcgcgcg cgcttgcgcc gcagacagcc
     3421 gcgctcgcag cgcggccaat agcgggatgc gcgcgaagtg cgaacggcgc gtgatgccgt
     3481 cgagatacgg ctcgagccag gcgaggtccg ccgcgagggc cgccggtgag aagtcgggcc
     3541 agtccgcgag atcgccgcgg ccgagcttgc gcacgaattc gttgcgtgcg gcgaaggtgt
     3601 gagcgtcgtc gtcccagggc agcgaatcga ggccgagtga ctgcaggccg tcgagcagtg
     3661 cggcgctcgt ggcttcggcg ggcaattcgg gcagcggctt ctcgtcgaga acgagctcgc
     3721 cgaagcgcac ggcgcgccgg gcgatgaccg cctccgcgcg cccgtcccac gcgacttcgt
     3781 cgcgcgtaac gatctcggac gcgaagtgct cgaggagatc gctgcgcgcg agcggcgcgg
     3841 cgagctggat ctgcgcttcg cgctcgcggt cgtcgagctc gacggccacg atgagctcct
     3901 cgcgtgcgat tgcctcgggg cccttgaacg acgcgccgcg gccgttggcg agctggtagc
     3961 ggcccgcgtc gccgggcctg cgcttgccga cccgatccgg gtacgcgaat gcgagcagcc
     4021 agccgacgtc gtgcgtatcc gtgcccgtat cgtcgccggc gccgacctcg cgcaggtagg
     4081 cgcgctcggc gcgccgcacg cgctcgagca cgccgcgatc gccgccgccg cgctgcaccg
     4141 tctcgagccg cgtgcggatg tcgctgtcgc gcgcgcccgc gcgcaacaag tcgcgctcgg
     4201 acagcagcgc cgcaagccgc gcggccgcgg gcgccgcgcc gtgctcg
//
LOCUS       DATLEE010000076         3867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5309119, whole genome shotgun sequence.
ACCESSION   DATLEE010000076 DATLEE010000000
VERSION     DATLEE010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5309119"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..2214)
                     /gene="fusA"
                     /locus_tag="VM692_01590"
     CDS             complement(88..2214)
                     /gene="fusA"
                     /locus_tag="VM692_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HUQ50881.1"
                     /translation="MARQKPLKLYRNIGIIAHIDAGKTTTTERVLYYTGKKHQIIEVH
                     DTKDGKTSTTTDYLEQERKRGITIQSAAVSCEWKGYQINLIDTPGHVDFTIEVNRSLR
                     VLDGAVVVFDGVAGVEPQTETNWRLANQYNVPRMCYVNKMDRMGANFTHCVKGIKERL
                     SAKSVVCQVPLGSYDEFCGMADLVAGQAYIWNSDDKDSTWETVPLDQLPGRFTFAAAG
                     DNEWVRRIPELRQELLEHALALNEDAFLEFVETGKFDPAVLKACIRKGTISGEFVPIL
                     CGSSFKNKGVQQMLDAVIDYLPFPGENGGIGMVDPDGKVIGTQEVKDDAPARALAFKV
                     INDQFGTLTFVRVYSGVIKKGDTLANVTRGKKERIGRIVEVQAAATKDIDECRAGDIC
                     AFVSMKETETGDSLGDPDKPALLERMRFPDPVISVSVEPKTRADVDKMSTALYKMVKA
                     DPSLRLEVDQETGQTVLKGMGELHLEVTIDRMRTELGVEANMGRPRVSFREAFGMPVE
                     LVYTHKKQSGGSGQYAEVKMIFEPQPAGTGVEFSDEVVGGRIPREFIPSVEHAIVTEC
                     KRGQVAGYEVVDFKARLIDGKYHDVDSSALAFEIAGRAAFREAHKTSRPKLLEPIMKI
                     EVVTEGEYLGTIIGDVNRRRGQVTDQGQKGNQATVVGFVPLSEMFGYINFLRSATSGR
                     GTFTMEFDHYSEVPPGLVQKLMDAEA"
     gene            2477..>3867
                     /locus_tag="VM692_01595"
     CDS             2477..>3867
                     /locus_tag="VM692_01595"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HUQ50882.1"
                     /translation="MKSRNWSSSWLLAGLLLAGCQPSDEELAARYEGVVGTYCLECHD
                     DAGREAGLTLEHVDLDNVAAHPDMFEEVARKLRGRIMPPLGGPRPDADTYDGFVAYLE
                     RRLDEAASSSPEPGQASIHRLNRTEYGNAVRDLVALDVDAAEFLPADDEAYGFDNIAD
                     VLRVSPSLLEQYLAASAKIAALAVGDPNTPAVTAVYRAPSDLAQASHIEGMPLGTRGG
                     IKIHHNFPLDGDYAFDVFLLRNIVGYMTGLEWAHELEIAIDGERVFLAQVGGEADNAM
                     SDANMSAAANEIDARLRTTTFVTAGPHDVTVAFLERSAAETHEPLELHTRNLDLQDMN
                     GLPVVDYVNVRGPFDAKGPGNTPSRERIFTCRPAEESTARVCATEILSNLAHRAYRRQ
                     PTEEDITTLLGFYDSGSARGGFDLGIESALRVILTSPKFLFRDEPDPEGVAPGSVYAL
                     DDTALASRLAFFL"
BASE COUNT          604 a         1265 c         1259 g          739 t
ORIGIN      
        1 gttcgcgttc gggttcccgg cgttcgcggc cgtgctcgcc atcctatggc tgatgacgag
       61 ccggccgatg ttctcgttcc gctgaggtta cgcctcggcg tccatgagct tctgcacgag
      121 gcccgggggc acttcggagt agtggtcgaa ctccatcgtg aaggtgccgc ggccgctcgt
      181 cgccgagcgc aggaagttga tgtagccgaa catctcggat agcggcacga agccgacgac
      241 cgtcgcctga ttgcctttct gaccttggtc cgtaacttgg ccgcgccggc ggttcacgtc
      301 gccgatgatc gtgcccaagt attcgccttc cgtgacgacc tcgatcttca tgatcggctc
      361 gagcagcttc ggccggctcg tcttgtgcgc ttcgcggaac gccgcgcggc ccgcgatctc
      421 gaaggcgagc gcggaggagt cgacgtcgtg gtacttgccg tcgatcagcc gagccttgaa
      481 gtcgacgact tcgtagcccg cgacctgacc gcgcttgcac tccgtgacga tcgcgtgctc
      541 gacgctcgga atgaactcgc gcggaatgcg gccgccgaca acctcgtccg aaaactcgac
      601 gccggtgccc gcgggctgcg gctcgaagat catcttcact tcggcgtact gaccgctacc
      661 gccggattgc ttcttgtgcg tgtagacgag ctcgacgggc atgccgaacg cctcgcggaa
      721 actgacgcgc gggcggccca tgttggcctc gacgccgagc tccgtgcgca tgcgatcgat
      781 cgtgacctcg aggtgcagct cgcccatgcc tttcaacacg gtctggcccg tctcttggtc
      841 gacctcgagg cgcagcgacg gatccgcttt gaccatcttg tagagcgccg tcgacatctt
      901 gtcgacgtcg gcgcgcgtct tcggctcgac cgagacgctg atgacggggt ccgggaagcg
      961 catgcgctcg agcagcgccg gtttgtcggg atcgccgagc gagtcaccgg tctccgtctc
     1021 tttcatcgag acgaacgcgc agatgtcgcc cgcgcggcac tcatcgatgt ccttcgtcgc
     1081 ggcggcttgc acctcgacga tacggccgat gcgctccttc ttgccgcgcg tgacgttggc
     1141 gagcgtgtcg cctttcttga tgacgcccga gtacacgcgc acgaacgtca gcgtgccgaa
     1201 ctggtcgttg atgaccttga acgcgagcgc gcgcgccggg gcgtcgtctt tgacttcctg
     1261 cgtgccgatg actttaccgt cgggatcgac catgccgatg ccgccgttct cgccggggaa
     1321 cggcaggtag tcgatgacgg cgtcgagcat ctgctgcacg cctttgttct tgaacgacga
     1381 gccgcacaag atcggcacga actcgccgct gatcgtgccc ttgcggatgc acgccttcag
     1441 cacggcgggg tcgaacttgc cggtctcgac gaactccagg aacgcgtctt cgttcaaagc
     1501 gagcgcgtgc tcgagcagct cttggcgcag ctcgggaatg cgccggaccc actcgttgtc
     1561 gccggccgcg gcgaaggtga agcggccggg cagctggtcg agcggcacgg tctcccaggt
     1621 gctgtctttg tcgtcggagt tccagatgta ggcctggccc gcgacgaggt cggccatgcc
     1681 gcagaactcg tcatagctgc cgagcggcac ttggcacacg acggacttgg cgctcaagcg
     1741 ctccttgatg cccttcacgc agtgcgtgaa gttcgcgccc atgcggtcca tcttgttgac
     1801 gtagcacatg cgcggcacgt tgtactggtt cgccaggcgc cagttcgttt ccgtctgcgg
     1861 ctcgacgccg gccacgccgt cgaacacgac gacggcgccg tccagcacgc gcaagctgcg
     1921 gttgacctcg atcgtaaagt cgacgtgccc cggggtgtcg atcaagttga tctgataacc
     1981 cttccactcg cacgaaaccg ccgcgctctg gatcgtgatg ccgcgcttgc gttcttgctc
     2041 gaggtagtcg gtcgtcgtgc tggtcttgcc gtccttggtg tcgtgcacct cgatgatctg
     2101 gtgcttcttg cccgtgtaat agagcacgcg ctcagtggtc gtggtcttgc ccgcatcgat
     2161 atgggcgatg atgccgatat tgcggtacag cttgaggggc ttttgcctgg ccataatgtc
     2221 gtcgtctcgt tcaaaataag ggatgcggcg gcgtcaatcg accgccgggg cactaaccaa
     2281 gagatgccgc gatcttaggg agctgaaggg gcggggcggc ccggccttcc agcggggcgc
     2341 cactgtaccc caaccgaagg ccactggcca agccaagacg gcgccggcgc tcatcttttt
     2401 taaaggtttc tagactggcg ccggcagttc cgctgcgtca ggctggaagc gtatttcgag
     2461 gtcgtttgcc gtgcttatga aaagccgaaa ttggtcgtcg tcgtggttgc tggcgggcct
     2521 gctgttggca ggctgccaac cgtccgacga ggagctagcc gcgcgctacg agggcgtcgt
     2581 aggcacctat tgcctcgagt gccacgacga cgccgggcgc gaagccggcc tgaccctaga
     2641 gcacgtcgat ctcgacaacg tcgcggcgca cccggatatg ttcgaggagg tcgcgcgcaa
     2701 gctgcgcggc cgaatcatgc cgccgctcgg cgggccgcgc cccgacgcag acacctatga
     2761 cggcttcgtc gcctacctcg agcgtcgctt ggacgaggcg gcctcgagct cgccggagcc
     2821 ggggcaggct tcgattcacc ggttgaaccg gaccgagtac ggcaatgccg tgcgcgacct
     2881 cgtggcgctc gacgtcgacg ccgcggagtt cctgcccgcc gacgacgagg cttacggctt
     2941 cgacaacatc gccgacgtgc tgcgcgtgtc gccgtcgctg ctcgagcagt atctggcggc
     3001 gtccgcgaaa attgccgcgc tcgccgtcgg cgaccccaac acgcccgccg tgacggcggt
     3061 gtaccgcgcg ccgtccgatc tcgcgcaggc gtcgcacatc gaaggcatgc cgctcggcac
     3121 gcgcggcggc atcaagatcc accacaactt cccgctcgac ggcgactacg cgttcgacgt
     3181 cttcttgctg cgcaacatcg tcggttacat gacgggcctc gagtgggcac acgagctcga
     3241 gatcgcaatc gatggcgagc gcgtgttcct cgcgcaggtc ggcggcgagg ccgacaatgc
     3301 gatgtccgat gcgaacatgt cggccgccgc caacgagatc gatgcgcggt tgcgcacgac
     3361 gacgttcgtg accgcaggcc cgcatgacgt gacggtggcg ttcctcgagc gcagcgcagc
     3421 cgagacgcac gagcccctcg agctgcacac gcgcaacctc gacttgcagg acatgaacgg
     3481 cttgcccgtc gtcgactacg tcaatgtgcg cgggccgttc gacgcgaagg gtcccggcaa
     3541 cacgccgagc cgcgagcgca tcttcacgtg tcggccggcc gaagagtcga cggcgcgcgt
     3601 ctgcgctacg gagatcctct cgaatttggc ccatcgtgcg tatcgccggc agccgaccga
     3661 ggaggacatc acgacgctgc tcggctttta cgactcgggg tcagcgcgcg gcggcttcga
     3721 cttaggcata gagtccgcgt tgcgcgtgat tctgacgagc ccgaagttct tgttccgtga
     3781 cgagccggat cccgaaggcg tggcgccggg ctcggtctac gccctcgacg atacggcgtt
     3841 ggcctcgcgc ctggcgttct tcctctg
//
LOCUS       DATLEE010000077         8142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3028220, whole genome shotgun sequence.
ACCESSION   DATLEE010000077 DATLEE010000000
VERSION     DATLEE010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3028220"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VM692_01600"
     CDS             <1..400
                     /locus_tag="VM692_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50883.1"
                     /translation="LLHEVGKLYILMQAKDTVDTLAADPGFQSVLAAWHPRLGRAVIE
                     AWELSAELAAAVGEHESCSLAAPDPPSMTSVVAVANYLVEHSEAACADPEFHAKAPDF
                     GALSLDKPTFDWLIRAADVDVRLLTISFGV"
     gene            475..1500
                     /locus_tag="VM692_01605"
     CDS             475..1500
                     /locus_tag="VM692_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50884.1"
                     /translation="MAFVIRVIASALLLASIAGGVTAQAPACDRRCLEGWIDRYLDAL
                     VKHDAKAVPLAATARYTENGQRLSVGDGLWRSVKAKGKYRLVVADTEAQQVAMIGTLE
                     EQNADPEMGTPAVVALRLKIVGSEIAEVEQFIARNIDAAKRIEALGRPHPLFTQPIPE
                     AERMSRADLIATANKYFTGMQQNDGKGDYPFAPDCNRIENGMQTTNRAAPPGEPRPNP
                     ATAANYSAQWSCREQFESGLLHFVTRIRDRRFVAVDPERGLVFSFVFFDHAAGDTRTF
                     TAANGRQVTAGPAQPWTWYIAELFKIEKGQLRQIEATLERVPYGMTSGWSSWEDGMSD
                     RPRDATR"
     gene            1525..2748
                     /locus_tag="VM692_01610"
     CDS             1525..2748
                     /locus_tag="VM692_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUQ50885.1"
                     /translation="MAAFSFNEFYQRNRKVVIWVILILLLWALREFFAVVFLSFVLAI
                     VAAPFAEVGMRRLKLPHWAAVTVVYVIFLLVLFSVVRFVVPSVATEVNRMMENLPATE
                     ATVIATKNDVVAKYPALREPINGYMRSALADDQLQLVELALDRDQQRLGLTEADLAAA
                     AESPAVPTGALAEYYSRQDQLLLNALMAVQRERLSQYAPIVTVMLYRATATTLLALLF
                     SYLILIDLNRIKAGISRLRTSRVGDFYEEAAPPIARFGILLGRAIEAQAVIAVINTVL
                     TLIGLLVLGIPLVAMLSVIVFFCSFVPVLGVFISTTPIVLVALNTGGPGLSLAAIGLV
                     IVIHAIEAYLLNPLIYGRHLKLNPVLTLIILYVGYHAFGLWGMLLGVPVARYFIHYVL
                     GVPFRERGRLARGPA"
     gene            2792..3781
                     /locus_tag="VM692_01615"
     CDS             2792..3781
                     /locus_tag="VM692_01615"
                     /inference="COORDINATES: protein motif:HMM:NF039296.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HUQ50886.1"
                     /translation="MSALRRLVGLAQRFPWLLPAVSFAAGWLGFLLVQRGQELARIMA
                     LLALLGWFWLLLEPWVRRRLERRRKRVGNFVVNFVTQSLQQELLFFSLPFLIGSTHLD
                     FGQIAFTSLVGVAALLSTIDPVYERVIATRAARRMSFHAFCSWIAALVVLPMVLLVPV
                     EQALPISLLAVTGWLLVTLPLSLYSLPRWPTRVAWVGGMIGVPLLFWAIRAQVPAAGL
                     AVTDARMTQTVDGLVPGVPVRRVRQVDLAQGVIAFAAIRAPAGLSQQVVFEWRHNGER
                     ERIPVEIHGGKADGFRAYSRKQIFPADSVGVWTVDVLTPQAQLLERLRFVVEG"
     gene            3863..4165
                     /locus_tag="VM692_01620"
     CDS             3863..4165
                     /locus_tag="VM692_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HUQ50887.1"
                     /translation="MERISADQLIDDLTAVIRDAENLLRATAAQTGDKVQEMRARAEE
                     SVRMAKERLSGIEEETLKRARAMAGEADEYVRGNPWQAVGIAAGLGLVLGLLMSRR"
     gene            4243..4590
                     /locus_tag="VM692_01625"
     CDS             4243..4590
                     /locus_tag="VM692_01625"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HUQ50888.1"
                     /translation="MAHLLATAIGIAQTRLELLSTELQEEVHRVAEIMLWAAVALLAA
                     GVGMFLLALLVIFVFWDTHRVLASIAVTCVFFAIAAVAGLTLGAKVRSKPPLLDATLA
                     ELKKDRAHLLSKS"
     gene            4599..4916
                     /locus_tag="VM692_01630"
     CDS             4599..4916
                     /locus_tag="VM692_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50889.1"
                     /translation="MSGRVKELLERERRLQERCAAQRASIAVEVAAIEARFVRVDRMA
                     GLARNALLNPFVIGGGIVALLTIGRLRGMRLIGRLYLLATAARRLMLVVRAIPRARVE
                     PEL"
     gene            4930..5985
                     /locus_tag="VM692_01635"
     CDS             4930..5985
                     /locus_tag="VM692_01635"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HUQ50890.1"
                     /translation="MSMLRFAAIAAVGSVAIGAHAQQNCAATGDVTYVCGPKNPEDLV
                     LVPGTQWIVSSGMADGAGFYLVDSRAGSFSTLTFTANHDHTFASCATPPSAQTLQTHG
                     LNIRAAGPGRARLNVVGHGAREAIEVFDIDASGARPTLTWRGCVPMPEGLAANSVASF
                     ADGSLVATVLFMPGTTFADAIVDRKPTGAVFEWSPGKPGFEQVRGAELPANNGIETSA
                     DGREIYVASSGLQTIVAFSHSNPARQLRTTQPLPFTPDNVHLAPDGRLVTAGMANDVP
                     ECGGPPGPNHDIEKLSKCPRPSIAMAVDPATMRTTVIATTTASPSFSNATMVLPVGNQ
                     AWLGSFSGDRIARAPLR"
     gene            complement(5991..6788)
                     /locus_tag="VM692_01640"
     CDS             complement(5991..6788)
                     /locus_tag="VM692_01640"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HUQ50891.1"
                     /translation="MTRRRLSPLATTVAAAAAVAACAWLGAARSQGAGDSRLDDAPTA
                     EFHFARLVYRNSPNSRRGGFGRRNGAWTTDYPDAEYHVMQGIGRFTGIDIASVDSDGS
                     GGRWIRLIDERVFDYPWLYAVEVGQWYLDDDEAKLLREYLDRGGFLMVDDFWGEDEWA
                     IFVDSMQKVFPDRRIQELGADHELLHVLYDLDQRTQIPGRGGQRPGTVPHWRGIFDDN
                     ERLMVAINFNMDMGDAWEHADDPWYPEPMTALAYRFAVNYLIYSMTH"
     gene            complement(6825..7670)
                     /locus_tag="VM692_01645"
     CDS             complement(6825..7670)
                     /locus_tag="VM692_01645"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ50892.1"
                     /translation="MHGPKSSLARAWYRLTTRRRLARNEWSAPYWEARGAERIVACRL
                     CPKLDAGRGECGVPFGSPLRKCVVAATEAHLRATRALRVLELGYARRSYGKRIVELAG
                     GSWTGVEPLLDRSVVPRLGSGGYGHAGDIAFPDATFDLVFGNQSFEHWEEPLPDGTAS
                     PSYAECLAEVWRVLKPGGTLYLDAPIHLHGHEMFVAGDVERIVSLFATELWTDVTVER
                     WRYAHEPLPRYPTPAADVGYVRGTIKSYDLAKIADLQSNGTVWLLAVTAVKRADVAAR
                     ARAGA"
     gene            7786..>8142
                     /locus_tag="VM692_01650"
     CDS             7786..>8142
                     /locus_tag="VM692_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50893.1"
                     /translation="MGTWRTAPSSVAVVVALACGGTAAAQDPVSELNGYLTLSSGYWR
                     HGLAQSDGASLQLGVDYQHYTGFFAYARAMNVDYPQNYEGQRRDVEGSAYVGFHRRRE
                     QWSWTVSLGKYFYPDAD"
BASE COUNT         1278 a         2667 c         2724 g         1473 t
ORIGIN      
        1 gctgctgcac gaagtcggca agctgtatat tttgatgcag gcgaaggaca ccgtagacac
       61 gctggccgcc gatccgggct tccaatccgt gttggcggct tggcatccgc gcctcggccg
      121 cgccgtcatc gaggcttggg aattgtccgc cgagctcgca gccgcagtcg gcgagcacga
      181 gagctgcagc ttggcggctc ctgacccgcc ttcgatgacg agcgtcgtcg cggtcgccaa
      241 ctatctcgtg gagcacagcg aggcggcctg cgccgatccc gagttccacg ccaaagcacc
      301 ggatttcgga gcgctgtcgc tcgataagcc gacgttcgat tggctcattc gcgctgccga
      361 cgtcgacgta cggctgttga cgatctcgtt cggggtctag cctcctcact gcccgcgcgg
      421 ggcggcgttc gcttaccata gccgtcccgg cgctcgcgcc cgtaggagga acgcatggcc
      481 ttcgtgattc gcgtcatcgc gagcgcgctg ctattggcct ccatcgcggg cggcgtcacg
      541 gctcaagccc cggcctgcga tcgacgctgt ctcgaggggt ggatcgaccg ctacctcgac
      601 gcgctcgtga agcacgacgc gaaagccgtg ccgctcgctg caaccgcgcg ctacaccgag
      661 aacggccagc ggttaagcgt cggcgacggc ttgtggcgca gcgtcaaagc aaagggcaag
      721 taccgactcg tcgtcgccga caccgaggcg cagcaagtcg ccatgatcgg cacgctcgag
      781 gagcagaacg ccgaccccga gatgggtacg cccgccgtcg tcgcgttgcg gctcaagata
      841 gtcggcagcg agattgcgga agtcgagcag ttcatcgcgc gcaacatcga tgccgcaaag
      901 cgcatcgaag cgctcggcag gccgcacccg ttgttcacgc agccgattcc ggaagcggag
      961 cgcatgtcgc gcgcggatct catcgcgacc gcgaacaagt atttcacggg catgcagcaa
     1021 aacgacggca agggcgacta cccgttcgcg cccgactgca atcgaatcga gaacggcatg
     1081 cagacgacga atcgagccgc gccgcccggc gagcctcgac ctaatcccgc aacggccgcc
     1141 aattactccg cgcaatggag ctgccgcgag cagttcgagt ctggtctctt acatttcgtc
     1201 acgcgcatcc gcgaccggcg gttcgtcgcc gtcgacccgg agcgcggctt ggtgttctcg
     1261 ttcgtcttct tcgatcacgc ggccggcgac acccgcacct tcacggcggc gaacggccga
     1321 caggtgacgg ccgggccggc gcaaccctgg acgtggtaca tcgccgagct gttcaagatc
     1381 gagaagggac agttgcgcca aatcgaagcc acgctcgagc gcgtgccgta cggtatgacg
     1441 tcgggctgga gcagctggga agacggcatg tcggatcgtc cccgcgacgc gactcgttga
     1501 ttctctaacc gcgtcgtagc gacagtggcg gccttcagct tcaacgagtt ctaccagcgc
     1561 aaccgcaagg ttgtcatttg ggtcatcctg atcctgttgc tgtgggcgct gcgcgagttc
     1621 ttcgcggtcg tgttcctgtc gttcgtgctc gcgatcgtcg ccgcgccgtt cgcggaagtc
     1681 ggcatgcgcc ggctcaagct gccgcactgg gctgccgtga cagtcgtcta cgtgatcttt
     1741 ctcctcgtgt tgttctcggt cgtccggttc gtcgtgccga gcgtggcgac cgaagtcaat
     1801 cgcatgatgg agaacctgcc ggcgaccgag gccacggtga tcgcgacgaa gaacgatgtc
     1861 gtggccaagt accccgcgtt gcgcgagccg atcaacggct acatgcgcag cgcgctcgcc
     1921 gatgaccagc tgcagctcgt cgagctcgcg ctggaccgcg atcagcagcg gctcggcctc
     1981 acggaggcgg acctcgccgc ggcggctgag agtccagccg tgccgacggg cgcgctcgcg
     2041 gagtactaca gccgccagga tcagctgttg ctaaacgcgc tgatggccgt gcagcgcgaa
     2101 cgcttgagcc aatacgctcc gatcgtgacg gtcatgttgt atcgcgcgac ggcaacgacg
     2161 ttgctcgcgt tgctgttcag ttatttgatt ctgatcgact tgaatcgcat caaagccggg
     2221 atcagccggc tgcgcacgtc gcgcgtcggc gatttctacg aggaggccgc tccgcctatc
     2281 gcgcgtttcg gcatactgct cggccgcgcg atcgaagctc aagcggtgat cgccgtgatc
     2341 aacacggtgc tgacgttgat cggcctgctc gtgctcggca ttccgctcgt cgcgatgctg
     2401 tccgtgatcg tgttcttctg cagcttcgtg cccgtgctcg gcgtgttcat ctcgacgacg
     2461 ccgatcgtgt tggtggccct gaacacgggc ggccccggcc tgtcactcgc ggcgatcggg
     2521 ctcgtcatcg tgatccatgc gatcgaggcg tatctgttga atccgctgat ctacggccgg
     2581 cacttgaagc tcaatcccgt gctgacgttg atcatcttgt acgtcggcta tcacgcgttc
     2641 ggcctctggg gcatgttgct cggcgtgccc gtcgcccgct atttcattca ctacgtgctc
     2701 ggtgtgccgt tccgggaacg cgggcgcctc gctcgcgggc cggcgtgagg atcgagtcgc
     2761 cgttcgacgc acgacgacgc gagtcacact gatgtccgcg ctgcgtcgtt tggtcggcct
     2821 tgctcagcgt tttccctggc tcctgccggc ggttagcttc gcggccggct ggctgggatt
     2881 cctgctcgtg cagcgcggtc aagagctcgc ccgcatcatg gcgctgctgg ctctgctcgg
     2941 ctggttctgg ttgctgctcg agccttgggt gcggcggcgg ctagagcgcc ggcgcaagcg
     3001 cgtcggcaac ttcgtcgtca acttcgtcac tcaatcgttg cagcaagagc tgctgttctt
     3061 cagcctgccg tttctgatcg gctcgacgca tctcgatttc ggccaaatcg cgttcacgag
     3121 cctcgtcggc gtcgctgcgc tcttgagcac gatcgatcca gtctacgagc gcgtcatagc
     3181 gacgcgtgcc gcgcgccgca tgtcgttcca cgcgttttgc agctggatcg cggcgctcgt
     3241 cgtgctgccg atggttctgc tcgtgcccgt cgagcaagcg ctgccgatct cgttgcttgc
     3301 cgtcaccggc tggctgctcg tgacactgcc gctgtcgctg tactcgctgc cgcgctggcc
     3361 aactcgcgtc gcgtgggtcg gcggcatgat cggcgtgccg ctgttgttct gggcgatacg
     3421 cgcgcaagtg ccggccgcgg ggcttgcagt gaccgacgcg cgcatgacgc agaccgtcga
     3481 cggcttggtg cccggcgtgc cggtacggcg cgtgcggcag gtcgatctcg cgcaaggtgt
     3541 catcgccttc gccgcgattc gtgcgccggc cggcctgtcg cagcaggtcg tcttcgagtg
     3601 gcgccacaac ggcgagcgcg agcggatccc tgtcgagatt catggcggca aggcagacgg
     3661 gtttcgcgct tactcccgga agcagatctt tccggcagac tccgtcggtg tctggacggt
     3721 cgacgtgctg acgccgcagg cacagctgct cgagcgcctg cggttcgtcg tagagggctg
     3781 agtcggcaca gattgtgctt gttacctcag aggtccggga cataccatct cttcaacgcg
     3841 cctttgggcg ggagtacttg caatggaacg aatttccgcc gatcagctca tcgacgattt
     3901 gacggccgtc atccgcgacg ccgagaacct gctgcgcgca acggctgccc aaaccggcga
     3961 taaggtccag gagatgaggg cacgcgccga ggagtcggtg cgcatggcga aggagcggct
     4021 gtcgggcatc gaggaagaga ccttgaagcg cgcgcgcgcc atggccggcg aggccgacga
     4081 gtacgtgcgc ggcaaccctt ggcaagccgt cggcattgca gcagggttag ggctcgtgct
     4141 cggcttgctg atgagccggc gctaaacgtg cccgtggacc cagccgtcga tagcattccc
     4201 gagcaaccct cacgcggccc gatcagcggg ctgtttcgct ccatggcgca cttgctggcg
     4261 accgcgatcg gtatcgcgca gacgcgtctc gagttgcttt cgacggagct tcaagaggaa
     4321 gtacaccgcg tcgccgagat catgctgtgg gccgccgtcg cgttgctcgc ggcgggcgtc
     4381 ggcatgttct tgctcgcgct cctggtcatc ttcgtgttct gggatacgca tcgcgtcctt
     4441 gcttcgatcg ccgtcacttg cgtgttcttc gcgatcgctg ccgtcgcagg tttaaccctc
     4501 ggcgcgaagg tgcgcagcaa gccgccgctg ctcgatgcga cgcttgcgga gctcaagaag
     4561 gaccgcgcgc acctgttgag caagagctga ggccgtcgat gagcggtcga gtcaaagagc
     4621 tgctggaacg agagcgccgc ctgcaggaac gatgtgcggc ccagcgcgcc agcatcgcgg
     4681 tcgaggtggc cgccatcgag gcccgcttcg tccgcgtcga tcgcatggcc gggctcgcgc
     4741 gcaacgcgct attgaacccc ttcgtcatcg gcggcggcat cgtcgcgcta ctgacgatcg
     4801 gccgcttgcg cggcatgcgg ctcatcggac ggctctatct gttggcaacg gcggcgcggc
     4861 ggctgatgct ggtcgtgcgc gccataccgc gtgctagagt cgagcccgaa ctttaacagc
     4921 gggggcacta tgagcatgct tcgtttcgcc gcgatcgcgg ctgtcggttc cgtggccatc
     4981 ggtgcacacg cccagcagaa ttgcgccgcc accggcgacg tcacctatgt ctgcgggccc
     5041 aagaatcccg aggatctggt tctggtaccg ggcacgcagt ggatcgtgtc gagcgggatg
     5101 gccgacggtg caggcttcta tctcgtcgat tcgcgcgccg gcagcttcag cacgctgacg
     5161 ttcacggcga accacgatca caccttcgcg agctgcgcga cgccgccttc ggcgcagacg
     5221 ctgcaaactc acggcctcaa cattcgcgcg gcggggccgg gccgagcgcg gctcaacgtc
     5281 gttgggcacg gcgcgcgcga agccatcgaa gtgttcgaca tcgatgcgag tggagcgagg
     5341 ccgacgctga cgtggcgcgg ctgcgtgccg atgcctgaag gtcttgctgc caacagcgtc
     5401 gcttcgttcg cggatggctc gctcgtcgcg acggtgctct tcatgcctgg cacgacgttc
     5461 gcggacgcaa tcgtcgaccg caagccgacc ggcgccgtgt tcgagtggtc gcccgggaaa
     5521 ccgggcttcg agcaggtccg cggcgcagag ctgccggcca acaacggcat cgagacctcg
     5581 gccgacggac gcgagatcta cgtcgcatcg tcgggtctgc aaacgatcgt cgcgttctcg
     5641 cacagcaatc cggcacggca gctgcgcacg acgcaaccgc tgccgttcac gcccgacaac
     5701 gtgcatctcg ctcccgacgg ccggctcgtg acggccggca tggcaaacga cgtgcccgag
     5761 tgcggtggcc cgccggggcc gaaccacgac atcgagaagc tctcgaagtg cccgcgcccg
     5821 tcgatcgcga tggctgtcga tcccgcaacg atgcgcacga ccgtgatcgc gacgacgacg
     5881 gccagcccga gcttcagcaa cgcgacgatg gtgctgcccg tcggcaatca ggcttggctc
     5941 ggctcgttct cgggcgaccg catcgcgcgc gcgccgttgc gttgacgcct ttagtgcgtc
     6001 atcgaataaa tgaggtagtt gacggcgaac cgatacgcga gcgccgtcat cggctcggga
     6061 taccacggat cgtcggcgtg ctcccacgcg tcgcccatgt ccatgttgaa gttgatcgcg
     6121 accatcaggc gctcgttgtc gtcgaagatg ccgcgccaat gcggcacggt gccggggcgc
     6181 tggccgccgc gccccggaat ctgcgtgcgt tgatcgaggt cgtagagcac gtgcagtagc
     6241 tcgtgatccg cgccgagctc ttggatgcgt cggtcgggaa ataccttctg catcgagtcg
     6301 acgaagatgg cccactcgtc ctcgccccag aagtcgtcga ccatgagaaa cccgccgcga
     6361 tcgagatact cgcgcagcag cttggcctcg tcgtcgtcga gataccactg gccgacttcg
     6421 accgcataca gccacgggta atcgaatacg cgctcgtcga tgagtcgaat ccaccgcccg
     6481 ccgctgccgt cgctgtcgac gctcgcgata tcgatgccgg tgaagcggcc gatgccctgc
     6541 atcacgtggt actcggcgtc cgggtagtcc gtagtccagg cgccgttgcg ccggccgaag
     6601 ccgccgcgac ggctgttcgg cgaattgcga tagacgaggc gcgcgaaatg aaattccgcc
     6661 gtcggcgcgt cgtcgagccg cgagtcgccg gcgccttggc tccgcgccgc gccgagccac
     6721 gcgcaagcgg cgacggccgc ggctgcggcg accgtggttg cgagtggcga gaggcgacgg
     6781 cgagtcacag agccgtagcc taacgcaggc gacggcttgc cgcgctacgc gccggctcga
     6841 gcccgcgcgg caacgtccgc gcgcttcacg gcggtcacag cgagcaacca aaccgtgccg
     6901 ttgctctgca agtccgcgat cttggccaag tcgtagctct tgatcgtgcc gcgcacgtaa
     6961 ccgacgtcgg ccgccggagt cggataacgc ggcaacggct cgtgtgcgta gcgccagcgc
     7021 tcgaccgtga cgtcggtcca gagctcggtc gcgaacaacg acacgatgcg ctcgacgtcg
     7081 ccggccacga acatctcgtg cccgtgcaag tgaatcggcg catcgagata cagcgtcccg
     7141 ccgggcttca gcacacgcca gacctcggcg aggcactcgg cgtacgacgg cgaagcagtg
     7201 ccgtccggca gcggctcttc ccaatgctcg aacgactggt tgccgaacac gaggtcgaac
     7261 gtcgcgtcgg ggaacgcgat gtcgccggcg tgcccgtagc cgcccgagcc gaggcgcggc
     7321 accacgctgc gatcgagcag cggctcgacg ccggtccatg agccgccggc gagctcgacg
     7381 atgcgtttgc cgtaggagcg gcgcgcgtag ccgagctcga gcacgcgcag ggcgcgcgtg
     7441 gcgcgcagat gtgcctccgt cgcggcgacg acgcatttgc gtaacgggct gccgaacggt
     7501 acgccgcatt cgccgcggcc cgcgtcgagc ttcgggcaga gtcggcaggc gacgatgcgc
     7561 tcggcgccgc gcgcttccca atagggtgcg ctccattcgt tgcgggcgag gcggcgcctg
     7621 gtcgtcaggc gataccacgc gcgggcgagc gagcttttcg ggccgtgcat cgtcggggtg
     7681 tcttcgttgg cgcctgatta tgacaccggg ggcgccgcgc gcaggtggtc aagcagcgcc
     7741 gcgcgggcct agaatcggtt cgggtccctg cgggggagcg catctttggg cacttggcgc
     7801 acagcgccgt cgtcggtcgc ggtcgtggtc gcgcttgcat gcggcggcac ggcagcggcg
     7861 caagatccgg tttccgagct caacggctac ttgacgctga gcagcggcta ttggaggcac
     7921 ggcttggcgc agagtgacgg cgcatcgctg cagctcggcg tcgactacca acattacacg
     7981 gggttcttcg cgtatgcgcg cgcgatgaac gtcgactatc cgcagaacta cgaagggcag
     8041 cggcgcgacg tcgaaggcag cgcgtacgtc ggttttcacc gtcgccgcga gcagtggtcg
     8101 tggaccgtca gcttgggcaa gtatttttat cccgacgcgg ac
//
LOCUS       DATLEE010000078         4849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7499036, whole genome shotgun sequence.
ACCESSION   DATLEE010000078 DATLEE010000000
VERSION     DATLEE010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7499036"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VM692_01655"
     CDS             complement(<1..275)
                     /locus_tag="VM692_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HUQ50894.1"
                     /translation="MNAVLHYIYDPLCGWCYGAEPLVSAASNVDGLALRMHAGGLWPQ
                     PTRLPDQTRRYIAQADARVGQMSGQPYGEPYLNGLLFDPELVLESRPV"
     gene            357..560
                     /locus_tag="VM692_01660"
     CDS             357..560
                     /locus_tag="VM692_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HUQ50895.1"
                     /translation="MNAPPLDPLASPCVRNCCLDEANVCMGCGRALDEIIAWGTASNA
                     DKAAILARSRERERLRAERLSRR"
     gene            complement(557..1201)
                     /gene="alkB"
                     /locus_tag="VM692_01665"
     CDS             complement(557..1201)
                     /gene="alkB"
                     /locus_tag="VM692_01665"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HUQ50896.1"
                     /translation="MATADRLSSQPEREELAPGACVLRGLALADEAELEGTLDAVAQQ
                     APFRQMITPGGFRMSVAMTNCGPYGWVSDEHGYRYAALDPVTGRPWPALPPVLAALAD
                     RAAALAGYAGFAPDACLVNRYEPGARLTLHQDKDERDFSQPIVSVSLGVPATFLFGGA
                     KRKERVARISLEHGDVVVFGGPSRLRYHGVSPLKPAHHPFAGQARINLTLRRSG"
     gene            complement(1180..2775)
                     /locus_tag="VM692_01670"
     CDS             complement(1180..2775)
                     /locus_tag="VM692_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HUQ50897.1"
                     /translation="MTDKHNELDRRLFMAGAVGAAAAVGAIGAADAQPPAGSPPAGGR
                     PPAGGPPRSSDATLSTLQQAQPRLYRLEGDVHDCEVTGKLPSDLSGAFYRVGPDAQYP
                     MRPGNIPFDGEGHVSMFRIKDGRVNYRSRFVRNERYLAQEKAGRILFPMYRNPAQDDP
                     SVKGLSRSTANTHIINHRNLLLSLKEDSPPSALNLLTLETVAPTYTFDGQLPSKTFTA
                     HPKLDSHTGNMVAFGYESQGHGSDIVTMFEFTPQGRKVWDAQIKVPYVGMLHDFAVTE
                     KYIVFYVIPLAIDQAQMDRGGIHWSWDGSKPTYFGFVRRGGDGKDVRWIQGPTRSATH
                     VMGAFDNAEKLVVDVEMSMSNPFPFMPMHDGSRWDPVAGSSHITRLTVDLKSRRPSAY
                     GIEQMYPHVGALPRQDDRYNTVPYRYGFLGCPDPMAAPGTGGACYARFDHQNRTWTLF
                     NAGTETTLAENVFAPKSKDAPEGAGYLMGLATRLTEGGRSDLVILDAERLQDGPVATV
                     HLPMRAPGQVHGWWVPEWQLPTV"
     gene            complement(2840..4231)
                     /locus_tag="VM692_01675"
     CDS             complement(2840..4231)
                     /locus_tag="VM692_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ50898.1"
                     /translation="MRTTIGVIVIAVGGAVGLTASLAGRPAVSQEARSVEALAARAAT
                     LEREITALEDVNAIKRLQRIYGFYTDKQLWSEAASLFAADGTIEVGGRGVYVGPERIR
                     AFLALNGPETPQPKRLFDHMQLQPVIDVAADGRTAQGRWHVFAQEAVHGEYANWGLGV
                     LENEYVKEDGVWKIRREHFFTTMYTPYADGWGKTALPNEAPSPQLAPDRPPTVAYEAY
                     PKALVVPFHYHHPVTTASANTPGTEQIMQRRVPEIASLGAFVDELDRRLGLLEDVAQL
                     ERLNSIYGYYLAHLQWDDLTGIFSPTGSIEIAMRGVYVGSASVRRNLNLYGQATDDQF
                     GLQHNHMQFQPVITVAPDGQSAKLRSRALSIMGNWERNSQWMGGVYENDYVKEDGVWK
                     IKRDQVFNTYFVPYTVGWKDVMPRPPPGITAANPPDLPPSHPFEMYPSAFLPPFHYAN
                     PVTGRTVTWEPPR"
     gene            complement(4241..>4849)
                     /locus_tag="VM692_01680"
     CDS             complement(4241..>4849)
                     /locus_tag="VM692_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ50899.1"
                     /translation="VASLEQRKQRIEDSNAIERLQGAYGYYVDRALWDDVANLFADDG
                     TIEIGLDGVYVGKARVREYLYALGGGREGLADGELSEHLQVMPVITLAPDGATAKARW
                     RAITLTGELGGEAFWGEGPYENEYVKDGGVWKIKTLHWYQALHVPYEGGWQANPDPTG
                     AKYVSSSLPPDRPPTIEYKTWPDTYVPPFSFPNPVGKYVAGE"
BASE COUNT          752 a         1630 c         1642 g          825 t
ORIGIN      
        1 acgggccgcg actcgagcac gagctcggga tcgaacaaga gaccgttcaa gtacggctcg
       61 ccatacggct ggccgctcat ttggccgacg cgcgcgtcgg cctgcgcgat gtagcgccgc
      121 gtttggtcgg gcaagcgcgt cggctgtggc cacagcccgc ccgcgtgcat gcgcagcgcg
      181 agaccgtcga cgttcgaagc ggccgacacg agcggctccg cgccgtagca ccagccgcaa
      241 aggggatcgt agatgtaatg caacactgcg ttcattgcgg cagtttacca gccggccgca
      301 acctaaggcg ccgttcacac acggggcgtg ccttcgtcgc ttatcatcgc gggcgcatga
      361 acgccccgcc gctcgatccg ctcgcgtcgc cttgcgtgcg caattgctgc ctcgacgaag
      421 ccaacgtctg tatggggtgc ggccgcgcgc tcgacgagat catcgcctgg ggcacggcaa
      481 gcaacgccga caaggccgcg atcctcgctc gcagccgcga gcgcgagcgc ctgcgcgccg
      541 agcgcctgtc gcggcgttag ccggagcggc gcaacgtcag gttgatgcgg gcttgccccg
      601 cgaacggatg gtgcgccggc ttcaacggcg acacgccgtg atagcgcagc cggctcggtc
      661 cgccgaacac gacgacgtcg ccatgctcga gcgagatgcg cgcgacgcgc tccttgcgct
      721 tcgctccgcc gaacaaaaag gttgccggaa cgccgagcga gacggagacg atcggttggc
      781 tgaagtcgcg ctcgtccttg tcctgatgca acgtgagccg cgcgccgggc tcgtagcggt
      841 tcacgaggca ggcgtcgggc gcaaagccgg cgtagcccgc gagcgcggcg gccctgtcgg
      901 cgagcgcggc cagcacgggc ggcagcgcag gccacggccg gcccgtcacg ggatcgagcg
      961 ccgcgtatcg gtagccgtgc tcgtcggaga cccagccgta gggtccgcag ttcgtcattg
     1021 cgaccgacat gcggaagccg cccggcgtga tcatctggcg aaacggcgcc tgctgcgcga
     1081 cggcgtcgag cgtgccctcg agctcggctt catcggccag cgcgaggccg cgcagcacgc
     1141 acgcgcccgg cgcgagctcc tcgcgctccg gctgagagct cagacggtcg gcagttgcca
     1201 ctccggcacc caccagccgt gaacctgtcc gggggcacgc atcggcaggt ggaccgtcgc
     1261 gacggggccg tcttgcaggc gctcggcgtc gaggatcacg agatccgagc ggccgccctc
     1321 ggtcaagcgc gtcgcgaggc ccatcaggta gccggcgccc tcgggcgcgt ctttgctctt
     1381 tggcgcaaag acgttctcgg cgagcgtcgt ctcggtccct gcgttgaaca gcgtccacgt
     1441 gcgattctgg tgatcgaagc gcgcgtagca ggcgccgccc gtgccgggcg cggccatcgg
     1501 atcggggcaa cccaagaacc cgtagcggta cggcacggtg ttgtagcggt cgtcctggcg
     1561 cggcagcgcg ccgacgtgcg gatacatctg ctcgatgccg tacgccgaag gccgccgcga
     1621 cttgagatcg accgtgagcc gcgtgatgtg gctcgagccc gcgacggggt cccaacgtga
     1681 gccgtcgtgc atcggcatga acgggaacgg attcgacatc gacatctcga cgtcgacgac
     1741 gagcttctcg gcgttgtcga acgcgcccat gacgtgcgtc gcgctgcgcg tcgggccttg
     1801 gatccagcgc acgtccttgc cgtcgccgcc gcggcgcacg aagccgaagt acgtcggctt
     1861 actgccgtcc cacgaccagt gaatgccgcc gcggtccatc tgcgcctgat cgatcgccag
     1921 cgggatcacg tagaagacga tgtatttctc cgtgacggcg aagtcgtgca gcatgccgac
     1981 gtacggcact ttgatctgcg cgtcccacac cttgcggcct tgcggcgtga actcgaacat
     2041 cgtgacgatg tcgctgccgt ggccttgcga ctcgtagccg aacgccacca tgttgcccgt
     2101 gtgcgagtcg agcttcgggt gcgccgtgaa ggtcttgctc ggcagctggc cgtcgaatgt
     2161 gtaagtcggc gcaacggtct cgagcgtcag caggttcagc gccgacggcg ggctgtcctc
     2221 tttcaacgac agcaacaagt tccggtgatt gatgatgtgc gtgttggccg tgctgcggct
     2281 caaacccttg acgctcggat cgtcctgcgc ggggttgcgg tacatcggga acaggatgcg
     2341 gcccgctttc tcctgcgcga gatagcgctc gttgcgcacg aagcggctgc ggtagttcac
     2401 gcggccgtcc ttgatgcgaa acatgctgac gtggccttcg ccgtcgaacg ggatgttgcc
     2461 gggccgcatc gggtactgcg cgtcgggtcc gacgcggtag aacgcgccgc tcaagtcgct
     2521 cgggagcttg cccgtgacct cgcaatcgtg aacgtcgcct tcgagccgat agaggcgcgg
     2581 ctgcgcctgc tgcagcgtcg acaaggtcgc atcgctcgac cgcggcggcc cgccggcagg
     2641 cggcctaccg ccggccggcg gcgaacccgc cggaggttgc gcatcggccg cgccgatcgc
     2701 gcccacagcg gcggcggcgc cgacggcgcc ggccatgaac agacggcgat cgagctcgtt
     2761 gtgcttgtcg gtcatcggaa tactccagca aagaagcggc gccgcggcgc gcgagatgcg
     2821 aatgatacta cccggtcggt tatcgtggcg gctcccacgt aacggtccgt cccgtcacgg
     2881 gattcgcgta atggaacggc ggtaagaacg cgctcggata catctcgaac gggtgactcg
     2941 gcggcaggtc gggcgggttc gcggccgtga tccccggcgg cggtcgcggc atcacgtctt
     3001 tccagccgac cgtgtagggc acgaagtacg tgttgaagac ttgatcgcgc ttgatcttcc
     3061 agacaccgtc ctccttgacg tagtcgttct cgtaaacgcc gcccatccat tggctgttgc
     3121 gctcccaatt gcccatgatg ctgagcgcgc gcgagcgcag ctttgcgctt tgcccgtccg
     3181 gcgcgaccgt gatcacgggc tggaattgca tgtggttgtg ctgcagaccg aactggtcgt
     3241 cggtcgcctg gccgtagagg ttcagattgc ggcgcacact ggccgagccg acgtagacgc
     3301 cgcgcatcgc gatctcgatg ctgcccgtcg gcgagaagat gccggtcagg tcgtcccact
     3361 gcagatgcgc aaggtagtag ccgtaaatcg agttcaggcg ctcgagctgc gcgacgtcct
     3421 cgaggaggcc aagtcgacga tcgagctcgt cgacgaaggc tccgagcgaa gcgatttccg
     3481 gcacgcgccg ctgcatgatc tgttccgtcc ccggagtgtt agccgacgcg gtcgtcaccg
     3541 gatggtggta gtggaacggc acgacgagcg ccttcggata cgcctcgtag gcgaccgtcg
     3601 gcggccgatc cggcgccagc tgcggcgacg gtgcctcgtt cggcagcgcc gtcttgcccc
     3661 agccgtccgc atacggcgta tacatcgtcg tgaagaagtg ctcgcggcgg atcttccaga
     3721 cgccgtcttc cttgacgtac tcgttctcga gcacgcccaa gccccagttc gcgtactcgc
     3781 cgtgcacggc ttcttgcgcg aagacgtgcc aacggccttg cgcggtccgg ccgtcggccg
     3841 cgacgtcgat gacgggctgc agctgcatgt gatcgaacag gcgtttcggc tgcggcgtct
     3901 cgggaccgtt caacgcgagg aacgcgcgga tgcgctcggg cccgacgtag acaccgcggc
     3961 cgccgacctc gatcgtgccg tcggccgcaa acagactcgc ggcttccgac cagagctgct
     4021 tgtccgtgta gaaaccgtag atgcgctgca gccttttgat cgcgttgacg tcctcgagcg
     4081 cggtgatctc ccgctcgagc gtcgcggcgc gcgccgcgag tgcctccacg gatcgcgctt
     4141 cctgcgagac ggccggtcgg ccggccagcg aggccgtgag cccaaccgcg ccgccaacgg
     4201 caatgacgat tacaccgata gtggtacgca tcgccgcccc ctactcgccc gccacgtact
     4261 tgccgacggg attcggaaag ctgaacggcg gcacgtaggt gtcgggccac gttttgtact
     4321 cgatggtcgg cggccggtcg ggcggcaacg agctcgacac gtacttcgcg cccgtcggat
     4381 ccgggttcgc ctgccaaccg ccctcgtacg gcacgtgcaa tgcctgatac caatgcagcg
     4441 ttttgatctt ccagacgccg ccgtccttga cgtactcgtt ctcgtacggc ccttcgcccc
     4501 agaacgcttc gccgccgagc tcgccggtta gcgtgatggc gcgccaacgc gccttggccg
     4561 tggcgccgtc gggcgccagc gtgatcacgg gcatgacttg cagatgctcg ctgagctcgc
     4621 cgtcggcgag accttcgcgg ccgccgccga gcgcgtagag atattcgcgc acacgcgcct
     4681 tgccgacgta gacgccgtcg agcccgatct cgatcgtgcc gtcgtcggcg aacaggttgg
     4741 cgacgtcgtc ccataaggcg cggtcgacgt agtagccgta cgcgccttgc agccgctcga
     4801 tcgcgttgct gtcctcgatg cgctgcttgc gctgctcgag cgatgctac
//
LOCUS       DATLEE010000079         4795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_839053, whole genome shotgun sequence.
ACCESSION   DATLEE010000079 DATLEE010000000
VERSION     DATLEE010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839053"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VM692_01685"
     CDS             <1..1017
                     /locus_tag="VM692_01685"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUQ50900.1"
                     /translation="GYQYAWLSADATLELVVGGHARLPSEPMTASTTLNAYSVAKTLT
                     AAAILELAAQQRLDLDAPLDSLLGFARSFNQKPTVRQTLAHLGGWPNPLPVSWIHLAA
                     EHAAFDRAQFVTRVLNDNPRLEKAPGRAFAYSNLGYLLLGVAIENVTRGRYEDYVEQR
                     LLRPLGLPDGATLSFTIPSSNRHAYGHIRRLGVLDLALGLFLERRKFAGRATGRWVEM
                     QPHYVNGAAYGGLIANAAGLGRYLQALLEPRGWLTPAAHDLLFAPVRTSAGVELRRSL
                     GWFRGTLGRHRYFAHAGGGAAYYCEARLYPELQRASVVMLNRAGLSDARLLDSLDRCL
                     LPAG"
     gene            complement(1024..1281)
                     /locus_tag="VM692_01690"
     CDS             complement(1024..1281)
                     /locus_tag="VM692_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50901.1"
                     /translation="MSNFKSQAKRKRELAKLDKRQAKDQKRALRKAERSGEAPAPEVA
                     VAPKPVHQPSNPTPTAARKPLTLAEAVERWKSSKVAKPNKR"
     gene            complement(1351..1704)
                     /locus_tag="VM692_01695"
     CDS             complement(1351..1704)
                     /locus_tag="VM692_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HUQ50902.1"
                     /translation="MGRKLYVGNLPYSATEQSLREAFAASGTVDSASLITDRDTGQSK
                     GFGFVEMATDAEAQAAIQAMNGKMLDGRQIKVNEAKPRESRGGGGGGGGRGGYGGGGG
                     GGGYGGGGGGYGRRS"
     gene            complement(1831..4227)
                     /locus_tag="VM692_01700"
     CDS             complement(1831..4227)
                     /locus_tag="VM692_01700"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HUQ50903.1"
                     /translation="MAYTLLGQDFTPPDLQAKVTGKAKYAEDFRAEGMLFCRLLTSTM
                     PHARVKFDATEALKMEGVVAILTADDVPPAQAPAEPILTNEPLFIGDRILAVAARSEE
                     LAAEAIERIKVEYEPLPYVIDPLASLFPAGPDARTDGNVVTAQGMKTVKWTAGDFSSA
                     GESTLPMGAAGAEWSFGDVDAGLAAAKYVLDESFVTAALSHHCLEPRSCMAYWQGDKV
                     FIYGSTQSQTAVMPNLARLAGVEPDNVVYIAEFCGGGFGSKINPYPSMGVPIYMAKKT
                     GMPVLLRINRHEEALFGSSRPAFQGRLRMGFRADGRVLAADLSIVQQNGPYSGGGDLG
                     AAAGAITLLYQPLAMRYRGIQVSTNTTPTGAQRGPGQNQIAAAVEPLLDKAAKALNLD
                     PLEIRRINAAGNDAKYDSQQGPVTSAYQREALERGAAAFDWNARKQRSGQQRGSKVRG
                     VGIGQAFHAAGRGGLDGIVRITPDGKLHVHCGVGNLGTYSYAATSRVAPEVLKVRWEN
                     VVIARGDSRLGLPFSSPQSGSNTSFTMTRATYAGAMDAVAKLKEIAAGVLGGAADDYD
                     IADETVFAKADRSKSMTYAAAAQRAIELGGKFAGHEVPEDINAVTKSGVAAIAGTGLI
                     GVAKDNIAVTGMVPAIAIGFIEIELDKETGKVDIVDYLGVADCGTVLHPMGLSAQIRS
                     GAVMGFGMGMSERHIYDPQNGLPGNIGMLQAKPASYLDVPSHMQTLAVDINDPQNPVG
                     SKGIGEPVEGCATAAVLAAISDALGGHYFNRAPVLPDMIVNAAAKREQSHKPLQVSTA
                     "
     gene            4367..>4795
                     /locus_tag="VM692_01705"
     CDS             4367..>4795
                     /locus_tag="VM692_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50904.1"
                     /translation="MNRPSVHTGLALAVGLVLALGAQAQSAKPIDSSEVPPRKQAAPL
                     PPRGTYVPARTSWGDPAIAGAYNNSDETGIPFERPEAFAGRTLESFTREELAQITAER
                     QRETIERNPTLSEFPGATSPMHWFENYFAANSRPWLVSDPP"
BASE COUNT          711 a         1649 c         1580 g          855 t
ORIGIN      
        1 ggctatcagt acgcgtggct gtcggccgac gcgacgctcg agctcgtcgt cggcggccac
       61 gcgcggctgc cgagcgagcc gatgacggcg agcacgacgc tgaacgctta ctccgtcgcg
      121 aaaactttga cggccgcggc catcctcgag ctcgccgcgc aacagcggct cgacctcgac
      181 gcgccgctcg attcgctgct cggcttcgcg cggtcgttca accagaagcc gaccgtacgc
      241 cagacgctgg cgcacttggg cggctggccg aacccgttgc cggtgtcgtg gatccatctc
      301 gccgccgagc acgcggcgtt cgatcgcgca caattcgtca cgcgcgtgtt gaacgacaac
      361 ccgcggctcg agaaagcccc gggccgagcg ttcgcctatt cgaatctcgg ctatttactg
      421 ctcggcgtcg cgatcgagaa cgtgacgcgc ggccgctatg aggactacgt cgagcagcgg
      481 ctgctgcggc cgcttgggtt gcccgacggc gcgaccttga gcttcacgat tccgagctcg
      541 aatcggcacg cctacggcca cattcgccgg ctcggggtgc tcgacctcgc gctcggtctg
      601 tttctcgagc gccgcaagtt cgcagggcga gccaccgggc gttgggtcga gatgcagccg
      661 cactacgtga acggcgcggc gtacggcggg ctgatcgcca acgccgccgg cctcggccgc
      721 tatctgcagg cgttgctcga gccgcgcggt tggttgacgc cggcggcgca cgacctcttg
      781 ttcgcgccgg tacgcacctc ggccggcgtc gagcttcgcc gtagcttggg gtggttccgc
      841 ggcacgctcg ggcggcatcg ctactttgcg catgccggcg gcggcgctgc gtactactgc
      901 gaggcgcggc tctatcccga gctgcagcgc gcgagcgtcg tcatgctgaa ccgcgccggg
      961 ctatcggacg cgcgcctctt ggactcgctc gatcgctgcc tcttgccggc cggctaacgt
     1021 ctctcaacgc ttgttgggtt ttgcgacttt cgagctcttc caacgttcca ccgcctcggc
     1081 aagcgtcagc ggcttgcgcg cggcagtggg tgtcgggttg ctgggttggt ggacgggttt
     1141 gggcgcgacg gccacctcgg gcgcaggcgc ctcgcccgac cgctcggcct tgcgcagcgc
     1201 gcgcttttga tccttggcct gacgcttatc gagcttggcg agctcgcgct tgcgctttgc
     1261 ctgcgacttg aagttggaca tggcactccc aagcgctgcg cggaggaccg agcctccgcg
     1321 cagcgtggtt cgagattcga tcgaaagcgg ttaagaccgt cggccgtagc cgccgccacc
     1381 accgccgtaa ccgccgccac caccaccacc gccgtaaccg ccgcggccac caccgccgcc
     1441 gccgccgccg cgcgattcac gtggcttggc ctcgttgacc ttgatctgac ggccgtcgag
     1501 catcttgccg ttcatggcct ggatggccgc ctgcgcttcc gcgtcggtcg ccatctcgac
     1561 gaaaccgaag cccttgcttt ggcccgtatc gcgatcggtg atcaagctcg cggaatccac
     1621 cgtgccgctt gcggcgaaag cctctcgcaa cgactgttcg gtggcggaat acggcaaatt
     1681 gccgacgtaa agttttctgc ccatggttac ctcctcaggt agcgttctcg cagcgagaga
     1741 ttggaggcac catgcccacc gactcgtccg aagcgacaaa gagcctacat actataggcg
     1801 ctttaccgtt cccaggtcga tcagttgcac tcaggccgtg ctgacctgca gcggcttgtg
     1861 cgactgctct cgcttcgcgg ccgcgttgac gatcatatcc ggtagcacgg gcgcgcgatt
     1921 gaagtagtga ccgccgagcg cgtcggagat cgccgccagc acggcggctg tcgcgcagcc
     1981 ctcgacgggc tcgccgatgc ctttcgagcc gacggggttc tgcgggtcgt tgatgtcgac
     2041 ggcgagcgtt tgcatgtgcg acggcacgtc gagataggac gccggcttcg cttgcagcat
     2101 cccgatgttg cccggcaagc cgttttgcgg gtcgtagatg tgccgctcgc tcatgcccat
     2161 gccgaagccc atgacggcgc cgctcctgat ctgcgcgctc aagcccatcg gatgcagcac
     2221 ggtgccgcag tcggcgacgc cgagataatc gacgatgtcg accttgccgg tctccttgtc
     2281 gagctcgatc tcgatgaagc cgatggcgat cgccggcacc atgccggtca cggcgatgtt
     2341 gtcctttgcc acgccaatca aaccggtccc cgcgatggca gcgacgccgg acttcgtcac
     2401 ggcgttgatg tcttcgggca cctcatgccc cgcgaacttg ccgccgagct cgatcgcgcg
     2461 ctgcgccgcg gccgcgtacg tcatgctctt gctgcggtcg gccttggcga acacggtttc
     2521 atccgcgatg tcgtagtcgt cggcagcacc gccgagcaca ccggccgcga tctctttgag
     2581 cttcgcgacg gcgtccatcg cgccggcata ggtcgcgcgc gtcatcgtga acgacgtgtt
     2641 gctgcccgat tgcggcgatg agaacggcag ccccaagcgc gagtcgccgc gcgcgatgac
     2701 gacgttctcc cagcgaactt tgagcacctc gggcgcgacg cgggacgtcg ccgcgtacga
     2761 gtacgtgccg agattgccga cgccgcaatg cacgtgcagc ttgccgtcgg gcgtgatgcg
     2821 cacgatgccg tcgaggccgc cgcggcccgc ggcatgaaac gcctggccga tgccgacgcc
     2881 cctgaccttc gagccgcgct gctggccgct gcgctgcttg cgcgcgttcc aatcgaacgc
     2941 cgccgcgccg cgctcgagcg cttcacgttg atacgcgctc gtgacggggc cttgctgcga
     3001 gtcgtatttc gcgtcgttgc cggcggcgtt gatgcggcgg atctcgagcg gatcgaggtt
     3061 caacgccttc gcggccttgt cgagcaacgg ctcgacggcg gccgcgatct ggttttgacc
     3121 ggggccacgt tgcgcgcccg tcggcgtcgt gttggtgctg acttgaatgc cgcgataacg
     3181 catcgcgagc ggctgataga gcagcgtgat ggcgccggcc gcggcaccga ggtcgccgcc
     3241 gccgctatac gggccgttct gctgcacgat cgacagatcg gcggcgagca cgcgcccgtc
     3301 ggcgcggaag cccattcgga gacggccttg aaacgcggga cgcgacgagc cgaacagcgc
     3361 ctcttcgtgg cggttgatgc gcagcagcac gggcatgccg gtcttcttcg ccatgtagat
     3421 cggcacgccc atcgacggat acgggttgat cttcgagccg aagccgccgc cgcagaattc
     3481 cgcgatgtag acgacgttgt cgggctcgac gccggccagg cgcgcgagat tcggcatgac
     3541 ggccgtctgg ctctgcgtcg agccgtagat gaacaccttg tcgccctgcc aatacgccat
     3601 gcagctgcgc ggctcgagac agtggtgcga caacgcggcc gtgacgaacg actcgtcgag
     3661 cacgtacttg gctgccgcta acccggcatc gacgtcgccg aacgaccact cggcgcccgc
     3721 ggcgcccatc ggcaacgtgc tctcaccggc tgaagagaaa tcgccggcgg tccacttgac
     3781 ggttttcatt ccctgcgccg tgacgacatt gccgtcggtt cgcgcgtcgg ggccggccgg
     3841 gaacaagctc gcgagcggat cgatgacgta cggcagcggc tcgtactcga ccttgatgcg
     3901 ctcgatggct tcggccgcga gctcttcgct gcgcgcggcg acggcgagga tgcgatcgcc
     3961 gatgaacagc ggctcgttcg tcaggatcgg ctccgccggc gcctgcgcgg gcgggacgtc
     4021 gtcggccgtc aagatcgcga cgacgccttc catcttcagc gcttcggtcg cgtcgaactt
     4081 gacgcgcgcg tgcggcatcg tgctcgtcag cagccggcag aacagcatgc cttcggcgcg
     4141 gaagtcctcc gcgtacttgg ccttgcccgt gactttggct tgcagatcgg gcggtgtgaa
     4201 atcctgaccg agaagcgtgt aagccatgag ctgtcccccc gctaggctcg cggccaggac
     4261 ccgcctgccg cttggtgtcg gccgattctg cggccgatac ggcaccgcat caaggtgctt
     4321 tccctacctc gccggtcggt gcggcggtgc ggtaggcttg agcgccatga atagaccttc
     4381 cgtgcacacc ggcctggcgc tggccgtcgg gctcgtcttg gccctcggcg ctcaggcgca
     4441 gagcgctaag cccatcgatt cgagcgaagt cccgccgcgc aagcaggcgg ctccattgcc
     4501 gccgcgcggc acctacgtgc ccgcccgcac gtcgtggggc gatccggcca ttgccggcgc
     4561 ctacaacaac agcgacgaga ccggcattcc gttcgagcgg cccgaggcat tcgcgggccg
     4621 tacgctcgag agcttcaccc gggaggagct cgcgcagatc acggccgagc gccagagaga
     4681 gacaatcgaa cgcaatccga cgctcagcga gtttccgggc gcgacgagcc cgatgcactg
     4741 gttcgagaac tacttcgccg ccaacagccg cccatggctc gtctccgacc cgccg
//
LOCUS       DATLEE010000080         8281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4563299, whole genome shotgun sequence.
ACCESSION   DATLEE010000080 DATLEE010000000
VERSION     DATLEE010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4563299"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..791)
                     /locus_tag="VM692_01710"
     CDS             complement(150..791)
                     /locus_tag="VM692_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HUQ50905.1"
                     /translation="MIVRRLLALLAAAPLLASCTTAYYRALETIGIEKREILVDRIED
                     ARDSQADAKEQFSSALDRYRSVVKVDGGDLEDIYDRLSAELERSEMRAKAVEDRVAAV
                     ESVADDLFDEWEGEVGQYSDPALRRQSQQLLTTTRGDYRRLIAAMRRADAAMDPVLTL
                     FNDQVLYLRHNLNARAIGSLETELADIERATQNLIAEMERSIAEASRFIDALA"
     gene            complement(788..1705)
                     /locus_tag="VM692_01715"
     CDS             complement(788..1705)
                     /locus_tag="VM692_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HUQ50906.1"
                     /translation="MSRGLWPLIKASVVAWRQDYASSMGGALAYYTLFSIAPVLIIVI
                     AVAGFFLGAEAARGEIVTQLRGLLGEEGAAAVQGLLESASKPEEGLFATLSSLALLLL
                     GATTVFAELQSDLDRIWRAETAKKISGLWSILRSRLLSFGLVLVLAFLLLVSLVLSAA
                     LAALGNWWGGWFEGWATLLEILNFVVSFAITMGLFALIYKFLPRTKIAWHDVWIGAAV
                     TALLFAIGKFLIGLYIGRSGIASGFGAAGSFVVLLIWVYYSTQIFLLGAEFTWVYAHE
                     RGSRRGRARPGPAPATPSTARPERAEVVQ"
     gene            complement(1867..2223)
                     /locus_tag="VM692_01720"
     CDS             complement(1867..2223)
                     /locus_tag="VM692_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50907.1"
                     /translation="MVQRPIDDDDPTSEEEALEFDGTHTDQLDDTYRFRLVASSESPN
                     GKVGFDDRGQSQWKWATELPASAVDGTGTFDQLKALENPALALEEVAPPAAEPPRKVG
                     YDPYATGVFEKPKTRG"
     gene            2454..2927
                     /locus_tag="VM692_01725"
     CDS             2454..2927
                     /locus_tag="VM692_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HUQ50908.1"
                     /translation="MFKKSNEMAAPPAFQMESDRRPSSKGASVLGPTLTFRGGELSSD
                     EDLIIEGTVEGKIAHQNHHLTIGKQDRVKANVRARLVTVYGTVEGDLHGDEGVHIAAS
                     ARVIGNVTAPRVSLENGAQFQGSIVTKEASSTASRAAAVEPAVDKLLGSGAATGR"
     gene            complement(2931..3326)
                     /locus_tag="VM692_01730"
     CDS             complement(2931..3326)
                     /locus_tag="VM692_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HUQ50909.1"
                     /translation="MKVGDSLEARFTVKQSDSAAALELSADDSFPNVFATSRLVALME
                     MAATRLMRPLLEPGQHSVGVLVSIKHTAPTPIGGHVRTVATYLGPEGKLFRFRVEAFD
                     DAGRIGDGEHTRAIVTTSRLLAGAAKRLP"
     gene            complement(3332..3772)
                     /locus_tag="VM692_01735"
     CDS             complement(3332..3772)
                     /locus_tag="VM692_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004354992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUQ50910.1"
                     /translation="MRNLDIGEVAEHCGVPPSTLRFYEEKGLIKSNGRRGLRRVFPGG
                     VLERLALIALGRAAGFSLDEIAAMFAPDGRLRIDRDKLAAKAKELDKTIGQLTAMRDG
                     LKHAVACKAPSHMECPKFRRIVQAAASGALGARRGKRRSALRRG"
     gene            3853..4704
                     /locus_tag="VM692_01740"
     CDS             3853..4704
                     /locus_tag="VM692_01740"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ50911.1"
                     /translation="MNTTTQQANADQIALWNGSAGRGWVEAQDSLDRLFEPFEELLVE
                     AVAASNARDVLDVGCGTGSTTLAAARRVGAGGTSLGVDISEPMIALARERAGLESTPP
                     KFLCADAQTYAFAPASFDLIVSRFGVMFFDHSVRAFANLLRATKPGGGLHVIAWRHPA
                     DNPFMTAAERAAAPLLPQLPARNADEPGQFAFADRERVRAILENSGWTEIDIRPLDVE
                     CVLPTSELDAYLIRLGPLARVLKQLDEPTRARVLTAVRAAFGPFVHGAEVRFTAACWT
                     IRARAAA"
     gene            4778..5521
                     /locus_tag="VM692_01745"
     CDS             4778..5521
                     /locus_tag="VM692_01745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUQ50912.1"
                     /translation="MSKSSASDAAGRRVAVTGAASGIGAATARLLQQRGWRVACLDRN
                     VAGARATAGAGGVAIEVDVSDEASCLDAFNQAGNAWGGLDALVTAAGIINTTPFFETT
                     VAEMRKLFDINVFGSFLSVREGARYMQKGGRICMVASISSYTGGGYVARGAYATSKGA
                     VLTMMKSCARELGPKGIAVNAVAPGFIDTPFVASAMNDPQRRKEVEAAVGKVGTAEQI
                     AECAAWLVSPAADFVHGETLIADGGILMR"
     gene            complement(5585..6391)
                     /locus_tag="VM692_01750"
     CDS             complement(5585..6391)
                     /locus_tag="VM692_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HUQ50913.1"
                     /translation="MGQYGSRGWRGPVPQPRLAAVSDECCVAANAAVAPRSKSSPRTH
                     TGWHRACLLLCAIKLTRRCVMRNKLAYAVLLGLMAGPALAQQNRDDERDRNDDRTGRN
                     FDGFYLGGGIGDFSSSVDEINKLDQIDDAGIDFSDGDNATKIFAGWNFNRFFAVQGDF
                     VDFGQSSGAVSTSTRGTSDVQGFAPSIVGTVPIGPIELFGRVGMMFYDVDLNLTGGRV
                     IDESGEDLVWSAGLGIDIKDRANLRLEYEKIDIAELDEADALWLNFAWKF"
     gene            complement(6411..6761)
                     /locus_tag="VM692_01755"
     CDS             complement(6411..6761)
                     /locus_tag="VM692_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUQ50914.1"
                     /translation="MRFISIFTHEPTNRGPTEAEMAAMGKLVEEAMKEGWLLSTEGVS
                     FGSKGFRVHKTAGGKITVTDGPFAEAKEVLGGYALMQAKSKEEILTYTRRFLEVAGQG
                     TCEIYQLYEMPSGN"
     gene            6906..7805
                     /locus_tag="VM692_01760"
     CDS             6906..7805
                     /locus_tag="VM692_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HUQ50915.1"
                     /translation="MEPRKPRKSEPNLDGDVHWGLDPQDVRRHARRMRQAQVVGPMGK
                     LVRVVAVVLVGAGAFLLTWNFTTLKQARLDFSELTALFARDSTPAGARPGEEPGTEVI
                     GDSSIAGGVSLPTSIKTGEPEANPPAAEPPTSEPAAPSPAPAPAPAQTETVRPSEPAP
                     PAVAAAQEEAARPAPPPREPEPERPVKPETIGFGLEVMNVSESDASAAILVLRDGGRR
                     QLSSFTWWVTGSEAIMGADFARLDRRVERFGVGEQNRTIYVPLVGDRAVEGPENFFVH
                     IAAGESMTAPETAKIEVIIKDDD"
     gene            complement(7802..>8281)
                     /locus_tag="VM692_01765"
     CDS             complement(7802..>8281)
                     /locus_tag="VM692_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HUQ50916.1"
                     /translation="ILYLSRYIIRNKADYYRLLLEVTTKQSWGPWISFMLDSIENTCG
                     WTTDKIKAIRDLMRHTADFMHRKLPKIYTWELVELLFKQPYCRISNVVDLGLAKRQTA
                     SVYLKSLVEIGVLRELNAGRESLFVHPKYIELLTGDENVWVYYAGVETDTELTAEEA"
BASE COUNT         1460 a         2782 c         2657 g         1382 t
ORIGIN      
        1 gtgcttcagc aacaccctga gcccgaatcg caggtcgtgg agcaagccta ggacctttgt
       61 cgcggagcgg cgcgccgtat tctcggctag ttatcgtccc tgccgcaacg tcgctgccgc
      121 gcgcgcgcct tgtgcgcgca gcgcgcgtat caggcgaggg cgtcgatgaa gcggctcgct
      181 tcggcgatcg accgctccat ctccgcgatc aggttctgcg tcgctcgctc gatatcggcc
      241 agctccgtct cgagcgagcc gatcgctcgc gcgttcaaat tgtgccgaag gtacaagacc
      301 tggtcgttga acagagttag taccgggtcc atggcggcgt cggcgcggcg cattgctgcg
      361 atcaaccggc gatagtcgcc gcgagtcgtc gtcagcagct gctggctttg ccgccgcaga
      421 gcgggatcgc tgtactgacc gacctcacct tcccactcgt cgaacagatc gtcggcaacg
      481 gattccaccg ctgcgacgcg gtcctccacg gctttcgcgc gcatctcgct gcgctcgagc
      541 tcggccgaga gccggtcgta gatatcctcg aggtcgccgc cgtcgacctt gacgacgctc
      601 cggtaacggt cgagcgccga gctgaactgc tccttcgcgt cggcctgcga atcgcgagcg
      661 tcctcgatgc ggtcgacgag gatttcgcgc ttctcgatgc cgatggtttc gagagcccgg
      721 taatacgccg tcgtgcacga cgccaagagc ggcgccgcag ccagcaatgc gagcaaacgc
      781 cggacgatca ttggacaacc tccgcgcgct cggggcgcgc cgttgacgga gtggcgggcg
      841 ccggcccggg gcgtgcgcgg ccgcgccggg acccgcgctc atgcgcgtag acccaggtga
      901 actcggcgcc gagaaggaag atctgcgtcg agtaatacac ccagatcagg agtacgacga
      961 acgagcccgc cgcaccgaaa cccgaggcga tgccgctgcg accgatgtac agcccgatga
     1021 ggaacttgcc gatcgcaaaa aggagcgctg tcaccgctgc gccgatccag acgtcgtgcc
     1081 aggcgatctt cgtgcgcggc aaaaacttat aaatcagtgc gaagagcccc atcgtgatcg
     1141 cgaacgacac gacgaaattt aggatctcga gcaacgttgc ccagccttcg aaccatccgc
     1201 cccaccagtt accgagcgcg gccaacgcag cgctcagcac gagcgacacg agcagcagga
     1261 acgcgagcac gagcaccaag ccgaacgaca acaaccggga acgcaggatc gaccagaggc
     1321 cactgatttt tttcgcggtc tccgcgcgcc agatccgatc gagatcgctt tgcagctcgg
     1381 cgaacaccgt cgtcgccccg agcaggagca gcgccaagct cgagagcgtc gcgaacagtc
     1441 cttcttcggg cttgctggcg ctttcgagca gaccttgcac cgccgcggcg ccttcctcgc
     1501 cgagcaggcc gcgcagttgc gtgacgatct cgccgcgggc cgcttcggcg cccaggaaga
     1561 aacccgcgac cgcgatgacg atgatgagca caggcgcgat tgagaacagc gtgtagtacg
     1621 cgagcgcgcc gcccatgctc gaggcgtagt cctgacgcca agcaacgacg gacgctttga
     1681 tcaagggcca taagccacga ctcatggagt tgcctcgctc gcttgcatga cgcttcaacc
     1741 atgcaaacga tgtgcctcac ggcgtccctg gcatgttcct tcgcgcgcgg cgttgcggca
     1801 catgaagccc ggcagctcga tcatcgcgac gagctcggag acagggatcc tggcggctga
     1861 agcgagttag ccgcgcgtct tgggcttttc gaacacgccg gtcgcgtaag gatcgtagcc
     1921 gaccttgcgc ggcggctcgg ccgcgggcgg tgcgacctcc tcgagcgcga gcgccggatt
     1981 ctcgagggct ttgagctgat cgaaggtgcc cgtgccgtcg acggcgcttg cgggcagctc
     2041 cgtggcccac ttccactgcg actggccgcg gtcgtcgaag ccgactttgc cgttcgggct
     2101 ctcgctcgag gccacgaggc gaaaccgata cgtgtcgtcg agctgatccg tgtgggtccc
     2161 gtcgaactcg agcgcttcct cttcggaagt gggatcgtcg tcgtcgattg gtcgctgcac
     2221 cataatatgc acgatgctag atgcaagccc cgtcaccttg cgtgacgaaa ctcacgggaa
     2281 aatcctgcca gcttcgtaac tacttgcatc gcgcaacatc tcggacggga ggcgctcgac
     2341 tgcccggccg acgccgggcg ctcgcgggtc gggctcggac tgtgagctac gtcgcggagt
     2401 gtttcgctgg aatggcgatg atcggtcttc cgcgtccctc gaggagccca ctcatgttca
     2461 aaaaatcgaa cgaaatggcg gcaccgcccg cgtttcagat ggaatcggac cgccgcccgt
     2521 cgtcgaaagg cgcgtcggtg ctcggaccca cgctgacgtt ccgcggcggc gagctgtcgt
     2581 ccgacgagga cctcatcatc gagggcaccg tcgaaggcaa gatcgcccac cagaaccacc
     2641 atctgacgat cggcaagcaa gaccgcgtca aagcgaacgt cagagcacgg ctcgtcacgg
     2701 tgtacggcac cgtcgaaggc gacttgcacg gcgacgaagg cgtgcacatc gcggcctcgg
     2761 cgcgcgtcat cggtaacgtg accgcgccgc gcgtctcgct cgagaacggc gcgcagttcc
     2821 aaggcagcat cgtgacgaag gaggcttcct ctacggcctc gcgcgcggca gcggttgagc
     2881 cggccgtcga caagctgctt ggctcgggag cggcgacggg gcgctgacct ctacggcaag
     2941 cgcttcgcgg cgccggccaa gaggcggctc gtcgtcacga tcgcgcgcgt gtgctcgccg
     3001 tcgccgatcc gccccgcgtc gtcgaacgct tcgaccctga accgaaacag cttaccttcg
     3061 ggcccgaggt aagtcgcgac ggtgcgcacg tgcccgccga tcggcgtcgg cgccgtgtgc
     3121 ttgatcgaga cgagcacgcc gaccgaatgc tgacccggct cgagcagcgg ccgcatcagg
     3181 cgcgtcgccg ccatctccat gagtgcgacg aggcgcgacg tcgcgaacac gttcgggaac
     3241 gaatcgtcgg ccgacagctc gagtgccgcc gcgctgtcgc tctgcttcac cgtgaagcgc
     3301 gcctctaaac tatcgccgac tttcatcgag cctaacctct tcgcagcgcg ctgcgcctct
     3361 tgccgcgtcg cgcaccgagc gcgccgctcg cggcggcctg cacgatgcgc cggaacttcg
     3421 ggcactccat gtggcttggc gccttgcagg cgacggcgtg cttcaagccg tcgcgcatcg
     3481 ccgtcagctg gccgatcgtc ttgtcgagct ccttggcctt cgccgcgagc ttgtcgcggt
     3541 ctatgcgcag gcggccgtcc ggcgcgaaca tcgccgcgat ctcgtcgagc gagaagccgg
     3601 ccgcgcggcc gagcgcaatc agcgcgagac gctcgagcac gccgcccggg aacacgcggc
     3661 gcagcccgcg ccggccgttc gacttgatga gccccttctc ctcgtagaac cgcagcgtcg
     3721 atggcggtac gccgcagtgc tcggcgactt cgccgatatc cagatttcgc atggggcttg
     3781 acctcaagtt gacttgaatt ggcagagtgc cttcacggtc cccgcaacgc aaccaaaaaa
     3841 aggagagcca ccatgaacac gacaactcaa caagcaaacg ccgaccagat cgctctgtgg
     3901 aacggcagcg ccggccgcgg ctgggtcgag gcgcaagact cgctcgaccg gctgttcgag
     3961 ccgttcgagg agctgctcgt cgaagcggtc gccgcgagca atgcacgcga cgtgctcgac
     4021 gtcggctgcg gcaccggcag cacgacgctc gccgccgccc gccgagtcgg cgcaggcggc
     4081 acgagtctcg gcgtcgacat ctcggagccg atgatcgcgc tcgcccgtga gcgagccggg
     4141 ctcgagagca cgccgccgaa atttctatgc gccgacgcgc aaacgtatgc ctttgcgccc
     4201 gcgagcttcg atctcatcgt gtcgcgcttc ggcgtgatgt tcttcgacca ttccgtgcgc
     4261 gcgttcgcga acttgctgcg cgccacgaaa cccggtggcg ggctgcacgt catcgcatgg
     4321 cgccacccgg cggataaccc tttcatgacg gcggccgagc gcgccgcggc gccgctcctg
     4381 ccgcagcttc cggctcgcaa cgccgacgag ccgggtcaat tcgcgttcgc ggatcgcgaa
     4441 cgcgtgcggg ccatcctcga gaacagcggc tggaccgaga tcgacattcg ccccctcgat
     4501 gtcgaatgcg tcctgccgac gagcgagctc gacgcatacc tgattcggct tgggccgctc
     4561 gcgcgcgtgc tgaagcaact cgatgagccg acgcgcgccc gagtgctcac ggccgtgcgt
     4621 gcggcgttcg ggccgttcgt gcacggcgcg gaagtccgct tcacggcggc gtgctggacg
     4681 attcgcgcgc gcgccgccgc gtgagccgca gtctggcagc cgagcgcaaa gccgactacg
     4741 cttcgcgcca cagcagcgaa cgcaaggaat tgcatcgatg agcaagagct cggccagcga
     4801 cgcagccggt cgacgcgtcg ccgtcacggg tgccgccagc ggcatcggcg ccgcgacggc
     4861 tcgtctgctt cagcagcgcg gttggcgagt cgcgtgcctc gaccgcaacg tcgcgggcgc
     4921 gcgcgccacg gccggcgcgg gcggcgtcgc aatcgaggtc gacgtctcgg acgaggcgtc
     4981 gtgcctcgac gcattcaacc aagccggcaa cgcttggggc gggctcgacg ctctcgtcac
     5041 ggcggcgggc atcatcaaca cgacgccgtt cttcgagacg accgtcgcgg aaatgcgcaa
     5101 gctgttcgac atcaacgtct tcggctcctt cttgagcgta cgcgaaggcg cgaggtacat
     5161 gcagaaaggt ggacgcattt gcatggtcgc gagcatctcg agctacacgg gcggcggcta
     5221 cgtggcgcgc ggcgcgtacg cgacgagcaa gggcgccgtg ctgacgatga tgaagagctg
     5281 cgcgcgcgag cttgggccga aaggcattgc cgtcaacgcc gtggcgcccg gcttcatcga
     5341 cacgccgttc gttgcgtccg cgatgaacga tccgcagcgg cgcaaggaag tcgaagcggc
     5401 cgtcggcaaa gtcggcacgg ccgagcagat cgccgaatgc gccgcatggc tcgtctcgcc
     5461 cgcggccgac ttcgtccacg gtgagacgct gattgccgac ggcggcattc tgatgcggtg
     5521 agcaaaacga ccgccgctgc ggcctaggcc gcagggcggt tctcaggttt tgctctcttg
     5581 aacgctagaa cttccaggca aagttgagcc agagcgcgtc agcctcgtcg agctctgcaa
     5641 tatcgatctt ctcgtactcg agccgcagat ttgcacgatc cttgatgtcg atgccgagac
     5701 cggccgacca aaccaagtct tcgccgctct cgtcgatgac gcggccgccc gtcaggttca
     5761 aatcgacgtc atagaacatc atgccaacac ggccgaacag ctcgatgggc ccgatcggca
     5821 ccgtacccac gatgctcggc gcgaagccct ggacatccga ggtgccccga gtcgacgtcg
     5881 agaccgcgcc gctcgactgg ccaaagtcca cgaaatcgcc ctgcacggcg aaaaaccggt
     5941 tgaaattcca gccggcaaag attttcgtcg cgttgtcgcc atcgctgaag tcgatcccgg
     6001 cgtcgtcgat ttgatcgagt ttgttgatct cgtcgaccga ggacgaaaaa tcgccgatgc
     6061 cgccgccgag atagaacccg tcgaaatttc gaccggtgcg gtcgtcgttg cgatcgcgct
     6121 catcgtcgcg attctgctga gcgagagcag gtcccgccat caatcccaag agcacggcgt
     6181 aagccagttt gttgcgcatc acacacctcc ttgtgagttt aattgcgcac agaagaaggc
     6241 aagctctgtg ccagccggtg tgcgtccgcg gggaactttt tgaacgcggc gcaacagcag
     6301 cgtttgcggc tacgcaacac tcgtcgctta ccgccgccag ccgcggctgc ggtactggcc
     6361 cacgccagcc gcggctgccg tactggccca cggcagccgc gaatcccgcc tcagttgccg
     6421 gacggcattt cgtagagctg gtagatctcg caggtccctt gccccgcgac ctcgaggaag
     6481 cggcgcgtgt acgtgaggat ctcctctttg gattttgctt gcatcagcgc gtaaccaccg
     6541 aggacttctt tagcctcggc gaatggcccg tcggtgacgg tgatcttgcc gccggcggtc
     6601 ttgtggacgc ggaagccctt ggagccgaag ctgacgccct cggtcgagag cagccaccct
     6661 tctttcatgg cctcctcgac gagcttgccc atggcggcca tttcggcctc ggtcggcccg
     6721 cggttcgtgg gctcgtgcgt gaaaatactg atgaagcgca tggtctgacc ctcctggggg
     6781 tgtgttgctt tcatctatac gtcgaacgac caaactacag atcgacacgc agcccaacaa
     6841 agttcctcgc ttcgcagccc cacgacgtct tggctcatcg agcaaccgcc agtactctca
     6901 aggccatgga acctcgcaag ccaaggaaat ccgagccgaa cctcgacggc gacgtgcact
     6961 ggggcctcga tccgcaggac gtacggcgcc acgcgcgacg aatgcgccag gcgcaggtgg
     7021 tcgggccgat gggcaagctg gtccgcgtcg tcgcagtcgt gctagtgggc gccggcgcgt
     7081 tcttgctgac ctggaacttc acgacgctga agcaggctcg cctcgatttc tccgagctga
     7141 cggcgctgtt cgcgcgcgat tcgacgcctg ccggcgcccg gcccggcgag gagcccggca
     7201 cggaggtcat cggcgactcg agtatcgcgg gcggcgtcag tctgccgacg agcatcaaga
     7261 ccggtgagcc cgaggccaat ccgcccgcgg cggaaccgcc gacgagcgag ccggctgcgc
     7321 catcgccggc acccgcgccc gcgcccgcgc aaaccgagac ggtgcgccca tccgagccgg
     7381 cgcccccggc cgtcgcggcg gcgcaggaag aagccgctcg ccccgcgccc ccgccgcgtg
     7441 agcccgagcc cgagcggccc gtcaaacccg agacgatcgg cttcggcttg gaggtcatga
     7501 acgtctcgga gtcggacgcc agcgccgcga tcctcgtgct gcgcgacggc ggtcggcggc
     7561 aactgtcctc cttcacgtgg tgggtgaccg gcagcgaagc gatcatgggc gccgacttcg
     7621 cgcggctcga tcgacgtgtc gagcgcttcg gcgtcggcga gcagaaccgt acgatctacg
     7681 tgccgctcgt cggcgaccgc gccgtcgagg ggcccgaaaa cttcttcgtg catatcgcgg
     7741 caggcgagtc gatgaccgcg cccgaaaccg cgaagatcga agtcatcatc aaggacgacg
     7801 attaggcttc ctcggccgta agctcggtgt ccgtctcgac gccggcgtag tagacccaaa
     7861 cgttctcgtc gcccgtcagc agctcgatgt atttcggatg cacgaacagc gactcgcggc
     7921 cggcattgag ctcgcgcagc acgccgatct ccacgagcga cttcaagtaa accgacgccg
     7981 tctgtcgttt ggctagcccc aagtcgacga cgttcgagat gcggcaatac ggctgcttga
     8041 acaagagctc gacgagctcc cacgtgtaga tcttcggcag cttgcggtgc atgaagtcgg
     8101 ccgtatggcg catcagatcg cggatggcct tgatcttgtc cgtcgtccaa ccgcacgtgt
     8161 tctcgatgct gtcgagcatg aacgagatcc acgggcccca gctctgcttc gtcgtgacct
     8221 cgagcagaag ccggtagtag tccgctttat tgcggatgat gtagcggctc agatagagaa
     8281 t
//
LOCUS       DATLEE010000081         3105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_95289, whole genome shotgun sequence.
ACCESSION   DATLEE010000081 DATLEE010000000
VERSION     DATLEE010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95289"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VM692_01770"
     CDS             complement(<1..578)
                     /locus_tag="VM692_01770"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ50917.1"
                     /translation="MFITKKHISRRTVLKGAGAAIALPLLDAMSPAGVAWGQTAAGKS
                     PHRLAFVGFPHGAVMRHFQPAETGRDYAMTRILEPLAPYREHMTIVTGLRNKPAESPE
                     PHAYIERTWLSCVSPKEAGTKGPDFGVTADQLAARHIGQETRIPSLELTTAGRGAQMA
                     WRTPTQSLPQDGNPRNVFYRLFGQGDNEAERAA"
     gene            complement(588..3035)
                     /locus_tag="VM692_01775"
     CDS             complement(588..3035)
                     /locus_tag="VM692_01775"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ50918.1"
                     /translation="MKRIRATDIVPNTLRAAALAAAASVLAACNPFGEQTVAERAPEQ
                     WPLVQRYCTDCHNGAELAGNLDFEKINPENVAQHAEKLEMAVRKLRSHAMPPPKEPRP
                     DEERLQSFVAWLEDALDEAAANNHGYQAIVPHRLNRQEYSNAVRDLLALDVAPDEFLP
                     QDEEVAHFDNIASGLQVSPSFIEQYVIAARAIAVRAVGRPDARTGSQTYTAPPGRQLS
                     HVRGLPLGTRGGFVAEHTFPSDGEYEVNIADMFSHIWGNDSEFENNVVVTLDGMVVYE
                     TKLGGEDDMKLYDQVQNGAMEKINARLKDIRFAATAGPHKVGVTFRRRTFAESDDQQQ
                     MFVPGGGQDRVFRVSSFEVSGPYNPTGLSATPSRDKIFVCHPSRGDDEELCAERILGD
                     LATRAYRRPLGEQDLQELLAYYRDGLAANGFEEGIRSGITGILASPYFLYRIETPPSN
                     VVPGETYAIDDVALASKLSFFLWNTIPDDELRELAAKGELSKEPVLRAQVARMLKDPR
                     AETLASNFVYHWLDLKRLEEVEPDTTIFPYASGSGDPREDYLKELELFSKSIFDEDRS
                     VVDFMTAKHTYVNERIALLYGINSVKGDRFQRVELEDSTRWGLLGKGAILMAAAYPNR
                     TSPVLRGAFILEYIAGAPPATPPPGVDAFPEAEIGTAKARTVREIMAKHRENPTCFSC
                     HGVMDPLGFALENFDAVGAWRDVDRYAGTPIDSAAELPDGTAVNGPDDLREALLRNPE
                     QFVQTFTERFLTYALGRTVDYRDMPAVRKIVREAGKQDYRFSELVWQVVASEPFRLRV
                     AAQAEQPVPVVAQAAAE"
BASE COUNT          487 a         1041 c         1020 g          557 t
ORIGIN      
        1 gcggcacgct cggcttcgtt gtcgccctga ccgaacaagc gatagaacac gttgcgcggg
       61 ttgccgtcct gcggcagcga ctgcgtcggg gtgcgccagg ccatctgcgc gccgcgcccg
      121 gccgtcgtca gctcgagcga cggtatgcgc gtctcctggc cgatgtgacg agccgcaagc
      181 tggtcggccg tgacgccgaa gtccgggccc ttcgttcccg cttccttcgg cgagacgcac
      241 gacaaccacg tccgctcgat gtacgcgtgc ggctcggggc tctcggccgg cttgttgcgc
      301 aggcccgtga cgatcgtcat gtgctcacga tacggcgcga gcggctcgag gatgcgcgtc
      361 atcgcgtaat cgcggcccgt ctcggccggc tgaaagtgcc gcatcacggc accgtgcgga
      421 aagcccacga aggcgagccg atgcggtgac ttgcccgccg cggtttgccc ccaggccacg
      481 ccggccgggc tcatcgcatc gagcagcggc aacgcgatcg ccgcccccgc tcccttcagc
      541 accgtccgac gcgaaatgtg tttcttcgtg atgaacatgt ctctctccta ctctgccgcg
      601 gcttgcgcga ccacgggcac gggctgctcg gcttgcgcgg ctacgcgcag acgaaacggc
      661 tcgctcgcca cgacctgcca caccaactcc gagaagcgat aatcctgttt gcccgcctcg
      721 cgcacgatct tgcgcacagc cggcatatcc cggtaatcga cggtccgccc caacgcgtac
      781 gttaggaagc gctccgtgaa cgtctgcacg aactgctcgg gattgcgcag cagcgcctcg
      841 cgcagatcgt cggggccgtt gacggccgtg ccgtcgggca gctcggccgc cgaatcgatc
      901 ggcgtgccgg catagcggtc gacgtcgcgc caagcgccga cggcatcgaa gttctcgagc
      961 gcgaagccga gcggatccat gacgccgtgg caggagaagc aggtcgggtt ctcacggtgt
     1021 ttggccatga tctcgcgcac cgtgcgcgcc ttcgccgtgc cgatttcggc ctcggggaac
     1081 gcgtcgacgc ccggcggcgg cgtggccggc ggcgcgccgg cgatgtactc gaggatgaac
     1141 gcgccgcgca gcacgggcga cgtgcggttc ggatacgcag cggccatcaa gatcgcgcct
     1201 ttgccgagca ggccccagcg cgtcgagtct tcgagctcca cgcgttggaa gcggtcgcct
     1261 ttgacgctgt tgatgccgta cagcagcgcg atgcgctcgt tgacgtaggt gtgcttcgcc
     1321 gtcatgaagt cgacgacgct gcggtcctcg tcgaagatgc tcttcgagaa cagctcgagc
     1381 tccttcaagt aatcctcgcg cgggtcgccg ctacccgagg cgtacgggaa gatcgtcgta
     1441 tctggctcga cctcctcgag gcgcttcaag tcgagccagt gatagacgaa gttgctcgca
     1501 agcgtctcgg cgcgcggatc ctttagcatg cgcgcgactt gcgctcgcag cacgggctcc
     1561 ttgctgagct cgccttttgc cgcgagctcg cgtagctcgt cgtcggggat cgtgttccac
     1621 aggaagaacg acagctttga cgccagcgcg acgtcgtcga tggcatacgt ctcgcccggc
     1681 acgacgttcg acggcggcgt ctcgatgcga tacaagaaat acgggctcgc gagaatgccc
     1741 gtgatgccgc tgcgaattcc ttcctcgaac ccattcgccg ccaagccgtc gcggtaatag
     1801 gccagcagct cctgcaagtc ctgctcgccg agagggcgcc ggtacgcgcg cgtcgcgagg
     1861 tcgcccaaga tgcgttccgc gcagagctct tcgtcgtcgc cgcgcgacgg atggcaaacg
     1921 aagatcttgt cgcggctcgg cgtggcgctc aaacccgtcg ggttgtacgg gccgctgacc
     1981 tcgaacgagc tgacgcggaa cacgcggtcc tggccgccgc ccggcacgaa catctgctgt
     2041 tggtcgtcgg actcggcgaa cgtgcgccgg cggaatgtca cgccgacctt gtgcgggccc
     2101 gccgtcgccg caaaccgaat gtccttcaac cgcgcgttga tcttctccat cgcgccgttc
     2161 tggacctggt cgtagagctt catgtcgtct tcgccgccga gcttcgtctc gtagacgacc
     2221 atgccgtcga gcgtcacgac gacgttgttc tcgaactccg agtcgttgcc ccagatatgg
     2281 ctgaacatgt cggcgatgtt gacttcgtac tcgccgtcgg acggaaacgt gtgctcggcg
     2341 acgaagccgc cgcgcgtgcc gagcggcagg ccgcggacat gcgacagctg acggccgggc
     2401 ggtgccgtat acgtctggct gcccgttcgc gcatcgggcc ggccgaccgc gcgcacggcg
     2461 atcgcgcgcg ccgcgatcac gtactgctcg atgaaagacg gcgacacttg taggccgctc
     2521 gcgatgttgt cgaagtgcgc gacctcttcg tcctgcggca agaactcgtc gggcgccacg
     2581 tcgagcgcca acaagtcgcg tacggcgttc gagtattcct ggcgattcag ccggtgcgga
     2641 acgatcgctt gataaccgtg attgttggcc gcagcttcgt cgagcgcgtc ctcgagccac
     2701 gcgacgaacg actgcagccg ctcctcgtcg ggccgcggct ctttcggcgg gggcatcgcg
     2761 tggctgcgca acttgcgcac ggccatctcg agcttctcgg cgtgctgcgc gacgttctcc
     2821 gggttgatct tctcgaagtc gaggttgccc gcgagctcgg cgccgttgtg acaatcggtg
     2881 cagtagcgct ggacgagcgg ccattgttcc ggcgcgcgct cggcaacggt ctgctcgccg
     2941 aacgggttac acgcggcaag aacgcttgca gcggcagcga gcgcggcggc gcgcagcgtg
     3001 ttgggaacaa tgtcggtcgc tcgaatccgt ttcacgattg agttcactct aagcatcggg
     3061 ggacaaatag gcccacaagc tctggaacgc ccgctccacg aatgc
//
LOCUS       DATLEE010000082         2121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3029224, whole genome shotgun sequence.
ACCESSION   DATLEE010000082 DATLEE010000000
VERSION     DATLEE010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3029224"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1093)
                     /locus_tag="VM692_01780"
     CDS             complement(29..1093)
                     /locus_tag="VM692_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50919.1"
                     /translation="MLKPKSLSLAMLGCAVAAALGGSGSALAQDAAPQGPQLTLVAPQ
                     GWKVPRLADGKPDIQGIWTNKTVTPFERPMDLGDKAYFTREEAEAYVQRTLARSNRDN
                     RTEDVNDVLSAYNAFWWDSGTSLLPNMRTSIVTKPANGRIPPLTAQRTAALNAQRDAQ
                     RARCERPGCAVANSGQLAPADEPQALDLMTRCISFGTVVPMLPSAYNNNYQIVQTAGT
                     VAINTEMVHQVRRIPTEPKPALPPQIRQWFGDPHGRWEGDTLVVESTNFTGEFFGRMT
                     AADENLRVTERFTLTAPDLLLYEFTVEDPTAWTGTWGGEIPLTRTDGLLYEYACHEGN
                     RGMENILRAARKELRQTSAR"
     gene            complement(1113..1907)
                     /locus_tag="VM692_01785"
     CDS             complement(1113..1907)
                     /locus_tag="VM692_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50920.1"
                     /translation="MSSSSDNLAATAVARVATCAVLALLSAAAVGQSARGAKPSFSGI
                     WRAASAANWDLEPHAAEPGPVPSLGTLLAVPPGPGVVVGGAIPYLPGALEQRNKNREQ
                     RWTADPEIKCFMPGVPRANYLPYPFQIVQGTDAIIMTYEFADAVRTVYLTDPGPAPAN
                     SWMGWNVGRFDGNTLVVDVTAQHDDTWLDRSGNYHSDELHVVERYTLRSPDVIDYEAT
                     IEDPQVFSRPWTIRLPLYRDTTENARLMEFKCIPFAEPVLYDKVGR"
     gene            complement(1904..>2121)
                     /locus_tag="VM692_01790"
     CDS             complement(1904..>2121)
                     /locus_tag="VM692_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ50921.1"
                     /translation="KDDGTVENWAIEGGTPNTLFRMGINQNSLKKGTEIVVDGYRSRD
                     GENRANGRDITLPDGRKLFLGGSAPPQ"
BASE COUNT          314 a          717 c          708 g          382 t
ORIGIN      
        1 ggccggccgg cactcgcccg cgagccgttc accgcgcgct ggtttggcgc agctccttgc
       61 gcgcggcgcg caggatgttt tccatgccgc ggttgccttc gtggcaggcg tactcgtaga
      121 gcagaccgtc ggtgcgcgtc agcggaatct cgccgcccca agttccggtc caagccgtcg
      181 ggtcttcgac cgtgaactcg tacagcagca ggtcgggcgc cgtcagcgtg aaacgctccg
      241 tgacgcgcag gttttcgtcg gccgcggtca tacggccgaa gaactcgccc gtgaagttcg
      301 tcgactcgac gacgagcgtg tcgccttccc agcgcccgtg cggatcgccg aaccactggc
      361 gaatctgcgg cggcagcgcc ggcttcggct cggtcgggat gcggcgcacc tggtgcacca
      421 tctcggtgtt gatggccacg gttccggccg tttgcacgat ctgatagttg ttgttgtacg
      481 cgctcggcag catcggcacc accgtgccga acgagatgca gcgcgtcatg aggtcgagtg
      541 cctgcggctc gtcggcgggg gccagctggc cgctgttcgc gacggcgcaa ccggggcgct
      601 cgcagcgcgc acgttgcgcg tcgcgctgcg cgttcagcgc ggcggtgcgc tgcgcggtca
      661 acggcggaat acgaccgttc gcgggcttcg tcacgatcga cgtccgcatg ttcggcaaca
      721 agctcgtgcc ggaatcccac cagaaggcgt tgtacgcgct cagcacgtcg ttgacgtcct
      781 ccgtgcggtt gtcgcgattc gaccgcgcga gcgtgcgctg cacgtaggct tcggcttcct
      841 cgcgagtgaa gtacgccttg tcgccgagat ccatggggcg ctcgaacggc gtcacggtct
      901 tgttcgtcca gattccctga atatcgggct tgccgtcggc gagccgcggc actttccaac
      961 cctgaggcgc cactagcgtc agctgcggac cctgcggcgc ggcgtcctgt gcaagcgcac
     1021 tgccgctgcc accgagcgcg gccgcgaccg cgcagcccaa catcgcgagc gacaatgatt
     1081 tcggcttcag catgtgatct cctaatcgcg cgtcagcgcc cgaccttgtc atacaagacg
     1141 ggctctgcga acggaataca tttgaactcc atgagccgcg cgttttccgt cgtgtcgcgg
     1201 tacagcggca agcggattgt ccaagggcgc gagaacacct gcgggtcttc gatcgtggcc
     1261 tcgtagtcga tcacatcggg cgagcgcagc gtgtaacgct cgacgacgtg cagctcgtcg
     1321 ctgtgataat tgccggagcg gtcgagccac gtgtcgtcgt gctgcgccgt gacgtcgacg
     1381 acgagcgtgt tgccgtcgaa gcggccgacg ttccagccca tccagctgtt cgcaggtgcg
     1441 ggccctggat ccgtcagata aaccgtgcgc acggcgtccg cgaactcgta cgtcatgatg
     1501 atcgcgtcgg tgccctgcac gatctggaac ggatacggca agtaattcgc tcgcgggacg
     1561 cccggcatga agcacttgat ctcggggtcc gccgtccagc gctgctcgcg gttcttgttg
     1621 cgttgctcga gcgcgcccgg cagatacggg atcgcgccgc cgacgacgac gcccggcccc
     1681 ggaggcaccg ccaacagcgt gccgagcgac ggcaccgggc ccggctcggc ggcatgcggc
     1741 tcaagatccc aattcgccgc gctggccgcg cgccagatgc cgctgaagct cggcttggcg
     1801 ccgcgcgccg actggccgac cgcagcggcg ctcagcagcg ccaacaccgc gcacgtagca
     1861 acgcgcgcaa ccgcagtcgc cgccaggttg tcgctgctcg agctcattgc ggcggcgccg
     1921 agccgccgag gaataacttg cggccgtcgg gcagcgtgat gtcgcggccg ttcgcgcgat
     1981 tctcgccgtc gcgcgagcgg tagccgtcga cgacgatctc ggtgcctttc ttcaacgagt
     2041 tctggttgat acccatgcga aacagcgtgt tcggcgtgcc gccttcgatg gcccagttct
     2101 cgaccgtgcc gtcgtccttg c
//
LOCUS       DATLEE010000083         4684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4565606, whole genome shotgun sequence.
ACCESSION   DATLEE010000083 DATLEE010000000
VERSION     DATLEE010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4565606"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VM692_01795"
     CDS             complement(<1..810)
                     /locus_tag="VM692_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HUQ50922.1"
                     /translation="MSAEVSKAPSRLALASAFAIVYVVWGSTFLAILFAIQSLPPFLM
                     ASVRFLVAGALLYAWSRFVSGAAAPSRAEWQSSAAVGALLLLGGNGLLVWSEQRIPSG
                     VAALLVGTVPCFMALIDWWRPGGTRPSRFVTAGLVLGLLGLAWLVGPDALMGGGRADL
                     VGATVVVLGSFSWAAGSIYSRHVARPASPFLSTAMQMLAGGAALLVLSVSLGELRLFD
                     VHAVTLRSALGLLYLIVLGSLVAFSAYVWLLRVSTPAKVSTYAYVNPVVAVL"
     gene            complement(859..2235)
                     /locus_tag="VM692_01800"
     CDS             complement(859..2235)
                     /locus_tag="VM692_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50923.1"
                     /translation="MVLPDQSASRSQARLAVFGVAAVVVLGAAAVWASLGAPHLVRSG
                     AHGDDTVLAGFAPTAPATGSVLRYETEYPRIPYLTGKRTDRVAQLISRIERGETKLTY
                     DSRRGYLDSLLAALDIDASSQTLVYSQTSLQSKLIGPKTPRAIYFNDDVYVGFVQQGP
                     IEIASLDDDLGPVFYLLEQNATAKPSFGAELGRCLSCHDSYSRSGGGVPRFIVGSGYT
                     GITGMLVSHEGWILVSDRTPLKSRWGGWYVTGRHGDQVHLGNMVIKTLYDFDKLEDLR
                     VGNRDTLDGLFDVSPYLTNESDIVALLVLQHQADVQNLITRVSYDARTAADKREEPLE
                     ETVERLLRMMLFVDAVEYTAPISGNPKFVEQFQSRAVRDSQGRSLRDFDLTRRLFRYP
                     LSYVIESSAFDALPADVKALFYRRLNEVLTGADSSNDFAHLSAADRDAIAAILRDTKP
                     DFAASVAN"
     gene            2301..3044
                     /gene="trxA"
                     /locus_tag="VM692_01805"
     CDS             2301..3044
                     /gene="trxA"
                     /locus_tag="VM692_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HUQ50924.1"
                     /translation="MDVTTATFEREVIEASKTSPVVVDFWAPWCGPCRALGPTLDKVT
                     ESFGGRVKLVKVNSDDNPELSQAFNIRSIPNVIAFKGGQAVAQFMGAIPEPQVREFFA
                     KLVPSAGEEALLAAETAFAAGRIDEAATLLTSVPRGDIATADRIATLEQGISFAQAGA
                     GGPSEAELREQIAIDPLDHDARIKLASVLAGKRRFREAMEELLEVVRLGKRDQADEAR
                     AQLLSVFTMAASDTKLVAEYRRKLASALH"
     gene            3047..3247
                     /locus_tag="VM692_01810"
     CDS             3047..3247
                     /locus_tag="VM692_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HUQ50925.1"
                     /translation="MQRTLITIGIAVLIAGLLWPWLSRLGLGRLPGDFRIQTGNSVFY
                     FPLATCLLVSIVLSLILWLLRR"
     gene            3229..>4684
                     /locus_tag="VM692_01815"
     CDS             3229..>4684
                     /locus_tag="VM692_01815"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HUQ50926.1"
                     /translation="MAAATVTSIRALCLAASFGLAAGALAQTDVEVIKVVGTTPLGAE
                     LDTDRIATNVQTATADELRERGALDLADFMNRSLGSVFVNEVQSNPLQPDVQYRGFVG
                     SPLLGLPQGLAIYQDGVRLNEPFGDTVNWALIPESAIASVHLVPGSNPLFGLNALGGA
                     IAIRTKDGINDAGTRAEVLTGSFDRLGLQVESGGALGDDLAYFITAARLTEDGWRDFS
                     PTEANQVFAKLTSTGERSHLEVSLTHADTELIGNGPLPVDLLEVDRKAIFTRPDITEN
                     HLNMLAVDAEQTVSDTIALRGNLYVRSSDIATLNGDDSDFDECRGSPGYLCELNDDGE
                     ELVLDENDLVIAADEALEGAAVNRTDTAQNGAGFALQADFSGPLAGRENQFTAGVSHD
                     DGDVEFSASTELGALDATRLALPGGVFVGEAFTELLASTTNTGFYVTDTLLLGKRTAL
                     TLSGRYNRTHIVLEDQLGTELNGDHSFERFNPALG"
BASE COUNT          832 a         1605 c         1554 g          693 t
ORIGIN      
        1 caggaccgcg acgacggggt tcacgtaggc gtaggtgctg accttcgccg gggtgctgac
       61 acgcagtagc cagacgtacg cgctgaacgc gacgagcgag ccgagcacga tcaggtagag
      121 aagcccgagc gcggagcgca acgtcaccgc gtgcacgtcg aacagccgca gctcgccgag
      181 cgagacactg agcaccagca gcgccgctcc gcccgcgagc atctgcatgg ccgtcgacag
      241 aaacggcgag gcaggccgcg cgacgtggcg cgagtagatc gagccggccg cccacgagaa
      301 cgacccgagc accacgaccg ttgcgccgac gagatccgcg cggccgccgc ccatcagggc
      361 gtcggggccg acgagccagg ccagcccgag caagccgagc accaaacctg ccgttacgaa
      421 gcgtgacggt cgagtgccgc ccggccgcca ccagtcgatc aaggccatga aacacggcac
      481 cgtgccgacg agcaacgccg cgactccgga cgggatccgc tgctcggacc agacgagcag
      541 accgttgccg ccgagcaaga gcaacgcacc gacggctgcc gatgactgcc attccgcgcg
      601 actgggtgcc gccgcgccac tgacgaagcg cgaccatgcg tagagcaacg cgcccgcgac
      661 gagaaaccgc acgctggcca tcaggaacgg cggcagcgac tgaatcgcga acaagatcgc
      721 cagaaaggtc gaaccccaaa cgacgtagac gatcgcgaag gcgctcgcca acgctagccg
      781 agacggagct ttggagactt cggcgctcat ggaggtgcag cgctggcggc cctcgggccg
      841 gtgcagcgca ggtctttttt agttggctac gctggccgcg aagtcgggtt tcgtgtcgcg
      901 cagaatcgcg gcgatggcgt cgcgatcggc ggcgctcaga tgggcgaagt cgttgctcga
      961 gtcggcgccc gtcagcacct cgttcaggcg ccgatagaac agcgctttga cgtcagcggg
     1021 cagcgcgtcg aatgcgctcg actcgatgac gtagctcagc ggataacgga acaggcgccg
     1081 cgtcagatcg aaatcgcgca gcgaccggcc ttgcgagtct cgcacggcgc ggctttggaa
     1141 ctgctcgacg aacttggggt tccccgagat cggcgccgtg tactcgacgg cgtcgacgaa
     1201 caacatcatg cgcaacaggc gctcgacggt ctcctcgagc ggctcctcgc gcttgtcggc
     1261 cgccgtgcgc gcgtcgtagc tgacgcgcgt gatgaggttc tgcacgtcgg cctgatgctg
     1321 cagcacgagc agcgcgacga tgtcgctctc gttcgtcagg tacgggctga cgtcgaacaa
     1381 gccgtcgaga gtgtcgcgat taccgacgcg caggtcctcg agcttgtcga agtcgtacag
     1441 cgtcttgatg accatgttgc cgagatgcac ttggtcgccg tggcgccccg tcacgtacca
     1501 cccgccccag cggctcttca gcggcgtgcg gtccgagacg aggatccagc cttcgtgcga
     1561 gacgagcatg cccgtgatgc ccgtgtagcc ggagccgacg atgaaccgcg gcacgccgcc
     1621 gcccgaccgc gagtacgagt cgtggcagct caagcagcgg ccgagctcgg cgccgaacga
     1681 cggcttcgcc gtcgcgttct gctcgagcag atagaacacg ggcccgagat cgtcgtcgag
     1741 cgaagcgatc tcgatcggcc cttgctgcac gaacccgacg tagacgtcgt cgttgaagta
     1801 gatcgcgcgc ggcgtcttcg ggccgatcag cttgctctgc aagctcgtct gcgaatagac
     1861 gagcgtctgc gacgacgcat cgatgtcgag cgccgcgagc agcgaatcga ggtagccgcg
     1921 ccgactgtcg taagtgagct tcgtctcgcc gcgctcgatg cggctgatca gctgcgcgac
     1981 gcgatcggtg cgtttgcccg tcagataagg gatcctcgga tactcggtct cgtagcgcag
     2041 cacggagccc gtcgccggcg ccgtcggcgc gaagcccgcg agcacggtgt cgtcgccatg
     2101 cgccccggac cgaacgaggt gcggcgcacc gagcgacgcc cagacggcgg cggcaccgag
     2161 cacgacgacg gccgcgacgc cgaacacggc gaggcgcgct tgagacctcg aggcactctg
     2221 atccggcaaa accatgcgct tagcttgcca aaacgtcgtc accacggcca cgtccgcagc
     2281 cctgcattac aatgtcggcc atggacgtca ctaccgctac gtttgagcgc gaggtcatcg
     2341 aagcctcgaa aaccagcccc gtcgtcgtgg atttctgggc gccatggtgc gggccgtgcc
     2401 gcgcgctcgg tccgacgctc gacaaggtga ccgagtcgtt tggcggccgc gtcaagctcg
     2461 tcaaggtcaa ctccgacgac aaccccgagc tctcgcaagc gttcaacatt cgctcgattc
     2521 cgaacgtcat cgcgttcaag ggcggccagg ccgtcgcaca gttcatgggc gcgatccccg
     2581 agccgcaggt ccgcgagttc ttcgcgaagc tggtcccgtc ggccggcgag gaagcgctgc
     2641 tcgcagccga gacggcgttt gccgcgggcc gcatcgacga agccgcgacg ctgcttacga
     2701 gcgtgccgcg cggcgacatc gcgaccgccg accgcattgc gacgctcgag caaggcatct
     2761 cgttcgcgca ggccggcgcc ggcggaccca gcgaagccga gctgcgcgag cagatcgcca
     2821 tcgacccgct cgatcacgac gcgcgcatca agctcgccag cgtgctcgcg ggcaagcggc
     2881 gctttcgcga ggcgatggag gagctgctcg aggtcgtgcg gctcggcaag cgcgatcaag
     2941 ccgacgaggc acgagcgcaa ttgctgtccg tgttcacgat ggccgcgtcc gacaccaagc
     3001 tcgtcgcgga ataccgccgc aagctcgcca gcgcgctgca ctagcgatgc agcgcacgct
     3061 gatcacgatc ggcatcgccg tcctgatcgc gggcctcctg tggccgtggc tctcgcggct
     3121 cgggctcggt cgactgcccg gcgacttccg catccagacc ggcaacagcg tcttctattt
     3181 tccgctcgcg acatgcttgc tcgtttccat cgtgttgtcg ctgattctat ggctgctgcg
     3241 acggtaacga gcattcgcgc gctgtgcctc gcggcgagct tcggcttggc cgccggcgcg
     3301 ctcgcgcaga cggacgtgga ggtcatcaaa gtcgtcggca cgacgccgct cggcgcggag
     3361 ctcgacacgg accgcatcgc gaccaacgtc cagacggcga cggccgacga gctccgagaa
     3421 cgtggcgcgc tcgatctcgc cgacttcatg aaccgcagct tgggcagcgt gttcgtcaac
     3481 gaggtgcaga gcaacccgct gcagccggac gtacagtacc gcggcttcgt cggctcgccg
     3541 ttgctcggcc tgccgcaagg cctcgcgatc tatcaggacg gcgtgcgcct caacgagccg
     3601 ttcggcgaca ctgtcaactg ggccctgatc cctgaaagcg cgatcgcgag cgtccatctc
     3661 gtgccgggct cgaacccgct gttcggcttg aacgcgctcg gcggcgcaat cgcgatccgc
     3721 acgaaggacg gcatcaacga cgcaggcacg cgcgccgagg tgctgacggg atcgttcgac
     3781 cggctcggcc tgcaggtcga gtccggcggc gcgctcggcg acgatctcgc ctacttcatc
     3841 acggcggcca gacttacgga ggacggctgg cgcgacttct cgccgacgga ggccaaccag
     3901 gtgttcgcga agctgacgtc gaccggcgaa cgctcgcacc tcgaggtcag cctcacgcac
     3961 gccgacaccg agctgatcgg caacgggccc ctgcccgtcg acctgctgga ggtcgatcgc
     4021 aaggcgatct tcacgcggcc cgacatcacc gaaaaccatt tgaacatgtt ggccgtcgac
     4081 gccgagcaga ccgtctcgga cacgatcgcg ctgcgcggca acctctacgt ccgctcgagc
     4141 gacatcgcga ccttgaacgg cgacgactcg gacttcgacg agtgccgcgg ctcgccgggc
     4201 tacctctgcg agctcaacga cgacggcgag gagctcgtgc tcgacgagaa cgacctcgtc
     4261 atcgccgcgg acgaggcgct cgagggggct gccgtcaacc gcacggacac ggcacagaac
     4321 ggcgccggtt tcgcgctgca agccgatttt tcggggccgc tcgcgggccg cgagaatcag
     4381 ttcacggccg gtgtctcgca cgacgacggc gacgtcgagt tctccgcaag cacggagctc
     4441 ggcgcgctcg acgcgacgcg gctcgcgctg ccgggcggcg tgttcgtcgg cgaagcgttc
     4501 acggagctgc tcgcctcgac gacgaacacc ggcttctacg tgacggacac gctgctgctc
     4561 ggcaagcgaa cggcattgac gctgtcgggc cgctacaacc gcacgcacat cgtgctcgag
     4621 gatcagctcg ggaccgagct caacggcgac cactcgttcg agcgcttcaa ccccgcgctg
     4681 gggc
//
LOCUS       DATLEE010000084         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1589063, whole genome shotgun sequence.
ACCESSION   DATLEE010000084 DATLEE010000000
VERSION     DATLEE010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589063"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VM692_01820"
     CDS             complement(<1..858)
                     /locus_tag="VM692_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ50927.1"
                     /translation="MSLRRLGSIVIKELRQLRRDRLTFAMIIGIPTMQLILFGYAINL
                     DVRNLETAVFDQAETARSRELISGMAATGVVHIGRVVSSTQEIDALLRAGEISAALVL
                     PPDFETRMERRDRPAVQIVVDGSEQVIQAAARQLAAYPLHDDRAWMNNGGGIEVVNFF
                     NPERRAAVNTVPGLVGMILTMTMVLFTGVAIVRERERGNLEMLIATPVSPWELTVGKI
                     VPFIGIGLVQTTVVLVLGDVLFDVPLRGSVLELYLAALVFITANLSLGITISTLTRTQ
                     FQAMQMSFFT"
     gene            complement(855..1775)
                     /locus_tag="VM692_01825"
     CDS             complement(855..1775)
                     /locus_tag="VM692_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ50928.1"
                     /translation="MHARGLSKHFGQVAAVDGLDLTVRKREVYGFLGPNGSGKSTTIR
                     MLCGLLNPTAGEINVLGCRIPQDAEALKRRIGYMTQKFSLYEDLTVDENLAFLAAVHD
                     MPRARAKPRIDELIEQFWLGKQRKQLAGTLSGGQKQRLALAGTVLHKPDLLFLDEPTS
                     AVDPQSRREFWDSLFQLADQGTTLLVSTHYMDEAERCTRLAILDRGRIVADGTPHELM
                     AGLPGRTLLVDTPEPRRAQQALRDLPGVIALAQIGTSLRVLAVNDPELRAHVTARLRS
                     AGVQGNVEPTAPNLEDVFVAATNKPQEKAA"
     gene            complement(1814..>2087)
                     /locus_tag="VM692_01830"
     CDS             complement(1814..>2087)
                     /locus_tag="VM692_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin secretion protein D"
                     /protein_id="HUQ50929.1"
                     /translation="VGDHPYARVFVPAPRRAELKIGARFEVRVEGVAAPFTASLRSIR
                     SEPSFTPYYALAGDDAARLVYPAELLLDGGEDLPAGLPLTAVPLAP"
BASE COUNT          330 a          698 c          724 g          335 t
ORIGIN      
        1 cgtgaagaac gacatctgca tcgcctggaa ctgcgtgcgc gtcagcgtcg agatcgtaat
       61 gccgagcgac aggttcgccg tgatgaacac gagcgcggcg agatagagct cgagcacgga
      121 gccgcgcagc ggcacgtcga acagcacgtc gccgagcacg agcacgacgg tcgtttgcac
      181 gaggccgatg ccgatgaacg gcacgatctt gccgaccgtg agctcccatg ggctgacggg
      241 tgtcgcgatc agcatctcga gattgccgcg ctcgcgctcg cgcacgatcg ccacgccagt
      301 gaacagcacc atcgtcatcg tcaggatcat gcccacgaga cccggcacgg tgttcacggc
      361 ggcgcgccgc tcggggttga agaagttcac gacctcgatg ccgccgccgt tgttcatcca
      421 cgcgcgatcg tcgtgcagcg gatacgcggc caattgccgt gccgcggctt ggatgacttg
      481 ctcggagccg tcgacgacga tctgcaccgc gggccggtcg cgccgctcca tgcgcgtctc
      541 gaagtcgggc gggagcacga gcgccgcgct gatctcgccg gcgcgcagca acgcgtcgat
      601 ttcttgcgtg ctcgagacga cgcggccgat gtgcacgacg ccggtcgcgg ccatgccgga
      661 aatcagctcg cgcgagcgcg cggtttccgc ttggtcgaac acggccgtct cgagattgcg
      721 gacgtcgaga ttgatcgcgt agccgaacaa gatcagctgc atcgtcggga tgccgatgat
      781 catcgcgaac gtcagccggt cgcggcgcag ttgccgcagc tccttgatga cgatcgagcc
      841 gagcctccgc agactcacgc ggctttctcc tgcggcttgt tcgtcgccgc gacgaacacg
      901 tcctcgaggt tcggcgccgt gggctcgacg ttgccctgca cgccggcgct tcgcaggcgc
      961 gcagtcacgt gcgcgcgcag ctccggatcg ttgacggcca gcacgcgcag cgacgtgccg
     1021 atctgcgcga gcgcgatgac gcctggcagg tcgcgcagcg cctgttgcgc gcggcgcggc
     1081 tcgggcgtgt cgacgagcag cgtgcggccc ggcagcccgg ccatgagctc gtgcggcgtg
     1141 ccgtcggcga cgatgcggcc gcgatcgagg attgcgagcc gcgtgcagcg ctcggcttcg
     1201 tccatgtagt gcgtcgagac gagcaacgtc gtgccctggt ccgcgagctg gaatagcgag
     1261 tcccagaact cgcggcgcga ctgcgggtcg acggcgctcg tcggctcgtc gaggaacagg
     1321 agatcgggct tgtgcaggac agtgcccgcg agcgcgagcc gctgcttctg gccgccgctc
     1381 aaggtgcccg cgagctgctt gcgctgcttc ccgagccaaa actgctcgat gagctcgtca
     1441 atgcgtggtt tcgctctggc gcgcggcatg tcgtgcacgg ccgcgaggaa ggccaaattc
     1501 tcgtcgacgg tcaggtcctc gtacaacgag aacttttgcg tcatgtaacc gatgcgccgc
     1561 ttcagcgcct cggcgtcttg cgggatgcgg cagccgagca cgttaatctc gccggccgtc
     1621 gggttcagca ggccgcacag catgcggatc gtcgtcgact tgccggagcc gttcggcccg
     1681 agaaagccgt agacctcacg tttgcgtacg gtcaggtcga gcccgtcgac ggccgcgact
     1741 tggccgaagt gcttcgacaa accgcgcgcg tggatcgcga tctcgctgcc attcgaccta
     1801 gccggtgtgc cgctcatgga gctagaggca ccgcagtgag gggcaacccg gcgggcaggt
     1861 cctcgccgcc atcgagcagc agctcggccg ggtaaacgag acgcgccgcg tcgtcgccgg
     1921 cgagcgcgta gtacggcgtg aagctcggct cgctgcggat gctgcgcagt gacgccgtga
     1981 acggcgcggc cacgccctcg acgcgcacct cgaaccgcgc cccgattttc agctcggcgc
     2041 gccgcggcgc cggcacgaac acgcgcgcgt acgggtggtc gccgacg
//
LOCUS       DATLEE010000085         2345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1589174, whole genome shotgun sequence.
ACCESSION   DATLEE010000085 DATLEE010000000
VERSION     DATLEE010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589174"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1306
                     /locus_tag="VM692_01835"
     CDS             209..1306
                     /locus_tag="VM692_01835"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HUQ50930.1"
                     /translation="MTVVTSFLSFIVTIVFMFALRPVAVAVGLVDIPGGRKRHDVPVP
                     IIGGVAMSIGLGIGTSLIEHPEFWNPTLLGVYLLVVVGTIDDRFDLPANVRLIAQSCA
                     ALLVVLASNVMVSDIGAPLFFGVSLGPLGLPFTVLFVMTLVNAFNLIDGIDGLAGGLA
                     LLSLLAMTIIGFGTPSFALASIAAAVVAGYLLFNLPLGFNKPVRAFMGDAGSTSLGLV
                     IAALGVYLSQAPVARISPVIGLWLVAVPVFDLFSTILRRLVEGKSPLAPDHEHLHHVL
                     VEHGLSRRATLACMLTLASVFAAVGITADIVVIPDGVMLVLWLAGGALYYQMMRHPKM
                     VVDVVRIALPAGQPRRALPPVLPRDSESRQD"
     gene            1407..>2345
                     /gene="wecB"
                     /locus_tag="VM692_01840"
     CDS             1407..>2345
                     /gene="wecB"
                     /locus_tag="VM692_01840"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HUQ50931.1"
                     /translation="MTARLAVDLIAAARPNFMKIAPLYHALAAESWCAPRIVHTGQHY
                     DANMSDAFFADLGMPKPHVHLGVGGGGHGEQTGRVMIEYEKVCVASRPNWIVVVGDVN
                     STLACALVGAKLQIPIAHLEAGLRSGDRRMPEEINRLATDAICDVLWTPSPDGDEHLA
                     HEGVPKDKIDRVGNIMIDSFELMAPKIRAAGMPAKIGVEKREYGVVTLHRPSNVDDAA
                     QLAAIVAKLAEISRRLPLVFPVHPRTRQRLKDSGLARTLEATPAIKLTEPLSYIEFMS
                     LVIDCRLALTDSGGVQEETSYLGIPCLTLRDNTERPI"
BASE COUNT          391 a          735 c          721 g          498 t
ORIGIN      
        1 cgtgaagaac gaaccaaacg acggtggtgg gaacgtgatg ccgctacccg tcgacgacta
       61 cacttgtata gaggccacaa tgtcgcgtcg ttgacagttt tcgtattgtc ttgcccaata
      121 acataacgta gatttcgctc agccgaagcc aggcctgcga acgttctgcg aacggtagcg
      181 taatttttgc cctgtcaggg gtctgcagat gacagtcgtg acgtcctttc tctccttcat
      241 tgtcacgatc gttttcatgt tcgcgctgcg tcctgtcgcg gttgccgtcg ggctcgtgga
      301 catacccggc ggccgcaaac gtcatgatgt gcccgtccca atcatcggcg gcgttgcgat
      361 gagcatcggt ctcggcatag ggacgagcct gatcgagcac cccgagtttt ggaatcccac
      421 tttgctcggc gtctatctgc tcgttgtcgt cggcacgatc gacgatagat tcgatctccc
      481 cgcgaacgtg cgtttgatcg cgcaatcgtg cgccgcgcta cttgtggtgc tcgcatcgaa
      541 cgtcatggtc tccgatatcg gcgcgccgct gtttttcggt gtgagcttag gtccgctcgg
      601 gctgccattc accgtgctgt tcgtcatgac attggtcaac gcgttcaacc tcatcgacgg
      661 cattgacggt ttggcaggcg ggctcgcgct gctgagcttg ctcgcgatga ccatcatcgg
      721 tttcggtacg ccgagctttg cgctcgcgag cattgcagcc gccgtcgttg ccggctattt
      781 gctcttcaat ttgccgctcg ggttcaacaa gcccgttcgc gcgttcatgg gcgacgccgg
      841 cagcacgtcg ctcggattgg tgattgcagc gctaggtgtg tatctgtcgc aggcgcccgt
      901 cgcgagaatc tcaccggtca tcggactctg gctcgtcgcc gtgccggtgt tcgatttgtt
      961 ctcgacgatc ttgcggcgcc tcgtcgaggg gaagtcgccg ttagcacccg atcacgagca
     1021 cctgcatcac gttctcgtcg agcacgggtt gtcacggcga gcgacgctgg cgtgcatgtt
     1081 gacgctggcg tcggtgttcg ccgcggtcgg catcacagcc gatatcgttg tcatacccga
     1141 tggcgtgatg ttggttctat ggctggctgg cggcgcgctc tactatcaaa tgatgcgcca
     1201 tcctaaaatg gtggttgacg tcgtccgcat cgcgttgccc gcgggccaac cgcgacgagc
     1261 gttgccccca gtgctgcctc gcgactcgga gtctcggcag gactagtcga cccggcttgc
     1321 ggatcgacgt gacgtaggct gtagcggggc agacgaactc tgtccacaat accgccccat
     1381 cgaacagcca ctcttaaagg tcgctagtga ctgcccgcct tgccgtcgat ctgatcgccg
     1441 ccgcccgtcc gaacttcatg aaaatcgcgc cgctctacca cgcgctcgcg gcggagtcgt
     1501 ggtgcgcgcc gcgaatcgtc cacacgggcc agcactacga cgcgaacatg tccgacgcgt
     1561 tcttcgcgga cctcggcatg ccgaaaccgc acgtgcacct gggcgtcggc ggcggcggtc
     1621 acggcgagca gacgggccgt gtcatgatcg agtacgagaa ggtctgtgtt gcttcgcggc
     1681 ccaattggat cgtcgtcgtc ggcgacgtga actcgacgct cgcgtgcgcg ctggtcggcg
     1741 ccaagctgca gatcccgatc gcgcatctcg aggctggctt gcgcagcggc gatcggcgca
     1801 tgcccgagga gatcaaccgc ctggccaccg acgcgatctg cgacgtgctc tggacgccgt
     1861 cgcccgatgg cgacgagcac ctcgcgcacg agggagtgcc caaggacaag atcgatcggg
     1921 tcggcaacat catgatcgac tcgttcgagc tgatggcgcc gaagattcgt gctgccggca
     1981 tgccggccaa gatcggcgtc gagaagcgcg agtacggagt agtgacgctg caccggccgt
     2041 cgaatgtcga cgatgcggcg cagctcgcag cgatcgttgc gaagctcgcc gagatctcgc
     2101 gtcgcctccc gctggtcttt cccgtacatc cgcgcacgag gcaacgcctc aaggactccg
     2161 gtcttgcgcg cacgctcgaa gcgacgcccg ccatcaagct caccgagccg ctgtcgtaca
     2221 tcgagttcat gagcctcgtc atcgactgta ggctcgcgtt gacggattcc ggcggcgttc
     2281 aagaggaaac cagttacctc ggcattcctt gcttgacgct gcgcgacaat accgagcggc
     2341 cgatc
//
LOCUS       DATLEE010000086         4237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5319553, whole genome shotgun sequence.
ACCESSION   DATLEE010000086 DATLEE010000000
VERSION     DATLEE010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5319553"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VM692_01845"
     CDS             <1..1234
                     /locus_tag="VM692_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUQ50932.1"
                     /translation="FASHSMRYALLKLEELFHKNVQSLFLSGPPGVGKTTLMRQRLRQ
                     MKDLSVTWVKPAIETREQLLTQLLEEIGPGAVEGSAAELRRILEVFLRHQAGNGRYAF
                     VVADGLERFAAPVLRELEALAQMRLRNRPILQLLTLTRSEELVTNLLPQYDGGPLARA
                     AHQRLAGFTLDETRSYIWACLRGAGCDWAEELVPDEVVLDVQAFTQGIVGDINAICGA
                     ALDAIAARSTGSNRQPKISRALLKEAGANLNLRYDAAAWTQPVEELSPEAVHLSDPSS
                     LRLEAARLIVSSGRTIVAEVTLNRPRMVLGRDDSCDISLDSRYVSRFQNLFLETPEGW
                     MLIDLSSTNGCFVNGRRVREHRLRDGDLIAVGHHQMRFTGPSGKTRAAAPAVQHSDDA
                     ETTLVGSRSETAERTTSH"
     gene            complement(1239..2510)
                     /locus_tag="VM692_01850"
     CDS             complement(1239..2510)
                     /locus_tag="VM692_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50933.1"
                     /translation="MNPRDSGIFRLASVGSYLDALSTLTDEMSLKERSNLIFLDRCLH
                     DRWGLGENVILNWSPEGDGIALLIVPHYAIARYASPQPDAARGVLGRPKQEFFQVLLS
                     GRRCLTPAQLAKVGRLLGIESTHLPLREPLKNTAAQNEIIEKMVKRYSITYVPNRGVC
                     LFDIVGFSLLSPFEQMMQLNSLSYSLNSAQSKLLTKRIGVDFSRTTTGDGFYIWNRDL
                     TLEGNVNLYHFMQLALADNAIAHKKAQHNTVPRLRACFHVGSSYEFHQSEGLSPTLYN
                     YIVGDVTVDLARVIERAMPGQILVGDFRAEMPAVDLPGSDSRIVESADFVELAMRNVE
                     QLAGLELSGERVDAIRCYLTGTKLASGEFTVRRITVNDKHGISRRVYNAKVNIYRHNA
                     EPILLGIEDRSLGSVGMPGYGSEQVLRPALA"
     gene            2661..>4237
                     /gene="ilvD"
                     /locus_tag="VM692_01855"
     CDS             2661..>4237
                     /gene="ilvD"
                     /locus_tag="VM692_01855"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HUQ50934.1"
                     /translation="MARKDNDSSGVDPRRHSRVVVDGLTQAPSRAMLRAVGFTDADFA
                     KPQVGIASTWNMVTPCNMHIDGLAKHAGAGADSAGAKSVLFNTITVSDGIANGTPGMR
                     YSLVSREVIADSIETVASAEGFDGLVTIGGCDKNMPGCVMAMARLDRPSIFVYGGTIQ
                     PGPKGRDIISVFEAVGSYAAGKIDDAELKEVESTAIPGPGSCAGMYTANTMASAIEAL
                     GLSLGNSSAQEAVSNAKADDCRRAGEAVVALIKRGIKPSDILTKEAFENAITTVIALG
                     GSTNAVLHLLAIAHEARVDLTLADFTRIGNRTPVLADLRPSGRYLMSELIRIGGIQPL
                     QKTLLDAGMLHGGCLTVTGRTLAENLASVRPYPAGQTIVRPLSDPIKADSHLAVLYGN
                     LAPEGAVAKISGKEGTRFEGRARVFNDERDALRAIIDGVVVGGDVIVIRYEGPKGAPG
                     MPEMLSPTGAVVGRGLSGKVALITDGRFSGGSHGFVVGHVTPEAAVGGPLAIVEDGDP
                     IVIDAVAKTIELGVSKET"
BASE COUNT          729 a         1392 c         1412 g          704 t
ORIGIN      
        1 gttcgcgtcg cattcgatgc gctacgcgct gctgaagctc gaggagctgt tccacaagaa
       61 cgttcagtcg ctgttcctga gcggccctcc cggtgtcggc aagacgacgc tgatgcggca
      121 gcgccttcga caaatgaagg acttgtcggt cacctgggtc aagcccgcga tcgagacgcg
      181 agagcagttg ctgacgcagt tgctcgagga gatcggcccc ggcgcagtcg agggctcggc
      241 ggcagagctg cggcgcatcc tcgaagtatt cctacgccac caagccggca acggccgcta
      301 cgcgttcgtg gttgccgacg gcctcgagcg gtttgccgcg cccgtgctgc gcgagctcga
      361 ggcgctcgca cagatgcggc tacgcaatcg gcccatcctg cagctgctga ccttgacgcg
      421 cagcgaggag ctcgtcacga atttattgcc gcaatacgac ggcggcccgt tggcgcgcgc
      481 ggcccaccag cgcttggccg gcttcacgct cgacgagact cgctcctaca tctgggcttg
      541 cttgcgcggc gccggctgcg actgggccga ggagctcgtg cccgacgagg tcgttctcga
      601 cgtccaagcg ttcacgcagg gcatcgtcgg cgacatcaac gcgatttgcg gcgcggccct
      661 cgacgccatt gcggctcgct cgaccggcag taatcggcag ccgaagatca gccgagcctt
      721 gctcaaggaa gcgggcgcca acttgaatct gcgctacgac gctgcggcgt ggacacagcc
      781 cgtcgaggag ctgtcgccgg aggccgtgca cctgagcgac ccgagcagcc tgcggctcga
      841 ggccgcgcgg ctcatcgtct cgagcggcag gaccatcgtg gccgaggtca cgctgaaccg
      901 gccgcgcatg gtgctcggcc gcgacgacag ctgcgacatc agcctcgaca gccgctacgt
      961 gagccggttc cagaacttgt tccttgaaac gccggaaggc tggatgctga tcgacttgag
     1021 cagcacgaac ggctgtttcg tcaacggccg cagagtccgc gagcatcggc tgcgcgacgg
     1081 cgacctgatc gccgtcggcc accatcagat gcgcttcacg ggtcccagcg gcaaaacgcg
     1141 cgctgcggcg ccggcggtac agcactccga cgatgcggag acgacgctcg tgggatcgcg
     1201 gtccgagacc gccgagcgga ccacgtcaca ctgacggttc aggccaacgc gggccgcaat
     1261 acctgctcgc tgccgtagcc cggcatccca acggacccca acgaccggtc ttcgatgccg
     1321 agcaagatcg gctccgcgtt gtggcgatag atgttgacct tggcgttgta gacgcgtcgc
     1381 gagatgccgt gcttgtcgtt gacggtgatt ctgcgcaccg tgaactcgcc gctcgcgagc
     1441 ttcgtgccgg ttaagtaaca gcggatcgca tcgacgcgct cgcccgacag ctcgagcccc
     1501 gctagctgtt ccacgttgcg catcgcgagc tcgacgaagt cggcggattc gacgatgcgc
     1561 gaatcgctgc ccggcagatc gacggccggc atctcggcgc ggaagtcgcc gacgaggatc
     1621 tgaccaggca tcgcccgctc gatgacgcgc gcaaggtcca ccgtgacgtc gccgacgatg
     1681 tagttgtaga gcgtcgggct caagccctcg gactggtgga actcgtagct gctgccgacg
     1741 tggaagcacg cccgcaagcg cggcacggtg ttgtgttgcg ccttcttgtg cgcgatcgcg
     1801 ttgtcggcga gcgcgagctg catgaaatgg tagagattga cgttgccttc gagcgtcagg
     1861 tcgcggttcc agatatagaa gccgtcaccc gtcgtcgtgc gcgagaagtc gacgccgatc
     1921 cgcttcgtca gcagcttgct ctgcgcggag ttcagcgagt acgacaggct attcagctgc
     1981 atcatctgct cgaacggcga caggaggctg aagccgacga tgtcgaacaa gcagacgccg
     2041 cggttcggca cgtaggtgat gctgtagcgc ttgaccatct tctcgatgat ctcgttctgc
     2101 gccgccgtgt tcttcaacgg ctcgcggagg gggaggtgcg tggattcgat gccgagcagg
     2161 cgcccgacct ttgcgagctg tgccggagtc aggcaacgcc gccccgacaa cagcacctgg
     2221 aagaactctt gcttcggcct gccaaggacg ccgcgcgcgg cgtcgggctg cgggctcgca
     2281 tagcgggcga tggcatagtg cgggacgatc agcagcgcga tcccgtcgcc ttcgggcgac
     2341 cagttcagga tgacgttctc gccgagcccc caacggtcgt gcagacagcg gtcgagaaag
     2401 atcagattgc tgcgctcctt caggctcatc tcgtccgtaa gcgtcgagag agcgtctagg
     2461 tagctcccga cgctcgccag cctgaagatt ccggaatccc tcggattcat cagcttccaa
     2521 aatgggtgag tggctggatc atacgctcgg ttcaacaggt ggggtagcgc gctgatttct
     2581 cgggctttag ggcctgcctg gacggccggt tacgcccccg ccataatcgc gtccccgctg
     2641 catacgcacc gacttctacc atggctcgta aggacaacga ctcctcaggc gtcgaccccc
     2701 gccgtcactc ccgcgtggtc gtcgacggcc tcactcaagc accgagccgc gccatgctgc
     2761 gagccgtggg gttcacggac gcggacttcg ccaagccgca ggtcggcatc gcgtcgacct
     2821 ggaacatggt cacgccgtgc aacatgcaca tcgacgggct cgcgaagcac gcaggggcgg
     2881 gcgccgatag cgccggcgcc aagagcgttc tgttcaatac gatcacggtg tcggacggca
     2941 tcgcgaacgg cacgccgggg atgcgttact cgctcgtgtc gcgcgaggtc atcgcagact
     3001 ctatcgagac cgtcgcctcg gcggagggct tcgacggcct cgtgacgatc ggcggctgcg
     3061 acaagaacat gcccggctgc gtcatggcga tggcgcgcct cgatcggccc tcgatcttcg
     3121 tctacggcgg cacgatccaa cccgggccga agggccgcga catcatctcg gtgttcgagg
     3181 ccgtcggcag ctatgccgcc ggcaagatcg acgacgccga gctcaaggaa gtcgagtcga
     3241 cggccattcc gggcccgggc tcctgcgccg gcatgtacac ggccaacacg atggcgtccg
     3301 cgatcgaggc cctgggcctg agcctcggga acagctcggc gcaagaagcc gtctcgaacg
     3361 ccaaggctga cgattgccgg cgtgccggcg aagccgtcgt ggcgctgatc aagcgcggga
     3421 tcaagccgtc ggacatcttg acgaaggaag cgttcgagaa cgcgatcacg accgtcatcg
     3481 cgctcggcgg ctcgacgaac gccgtgctgc acttgctggc catcgcgcac gaggcgcgcg
     3541 tcgatcttac gctcgcggac ttcacgcgca tcggcaatcg cacgccggta ctcgcggacc
     3601 tgcggccgag cggccgctat ctgatgtcgg agctgatccg catcggcggg attcaaccgt
     3661 tgcagaagac gctgctcgac gccggcatgc tgcacggcgg ctgcctgacg gtcacgggcc
     3721 gcacgctggc cgagaacctc gcgagtgtgc ggccgtatcc ggccgggcag accatcgttc
     3781 gccccttgag cgatccgatc aaggccgaca gccacctcgc cgtgctctac ggcaacctcg
     3841 ctcccgaagg cgccgtcgcg aagatttcgg gcaaggaagg cacacggttc gaaggccgcg
     3901 cgcgggtgtt caatgacgag cgcgatgcgc tgcgcgcgat catcgacggc gtcgtcgtcg
     3961 gcggcgacgt catcgtgatc cgttatgaag ggccgaaggg cgcacccggg atgcccgaga
     4021 tgctgagccc gacgggcgcc gtcgtcggcc gcggcctgag cggtaaggtc gcactgatca
     4081 ccgacggccg attctccggc ggcagccacg gcttcgtcgt cggtcacgtg acgcccgaag
     4141 cggccgtcgg tgggccgctc gcgatcgtcg aggacggcga cccgatcgtc atcgacgccg
     4201 tggccaagac gatcgagctc ggcgtgtcga aggagac
//
LOCUS       DATLEE010000087         6559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6758800, whole genome shotgun sequence.
ACCESSION   DATLEE010000087 DATLEE010000000
VERSION     DATLEE010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6758800"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VM692_01860"
     CDS             complement(<1..1084)
                     /locus_tag="VM692_01860"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HUQ50935.1"
                     /translation="MPSRDPVLLALDDDQDIVEAICAIGARAGFRAIPSTSAARLREL
                     VAGERPDVIILDLQMPGEDGVSALRHLADVECAAKIFLVTGMDERTIASAEQYGLRRG
                     LAVFGTLQKPFDPDELFAGLEKAHAAIRPLTAADLEQAIEREQLLVHYQPIIRRFADN
                     TWDIAAVEALLRWDHPIRGLLTPDSFVSLGEAHGLSRPMTDFVLQRGIEQLKGWETQR
                     LRIGLRVNIAATLISDIGFPDRLETMLVEQSVDAESLALEITETAMLEQTPQAFDILT
                     RLRIKNIGLAIDDFGIGYSSLTQLFRMPFNEMKIDRSLVAKIGESKEASIMVDALVSL
                     AHKLNLTVCAEGVENRATLDALARFGC"
     gene            complement(1087..1350)
                     /locus_tag="VM692_01865"
     CDS             complement(1087..1350)
                     /locus_tag="VM692_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HUQ50936.1"
                     /translation="MLVVSRKENESIRIEPVAGLDPALTLREAFAGGAITVRLQSVGQ
                     KRVRLVIEAPAALKIMRTEVPCSESAADGERGAQPSVARAGQG"
     gene            1659..2102
                     /locus_tag="VM692_01870"
     CDS             1659..2102
                     /locus_tag="VM692_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HUQ50937.1"
                     /translation="MTTSDGGSAPAADEALRAVLAFYRLSRSLRNTDLRDLTWERLST
                     LSVVARREPVSISELSVVEEVTAPTISRTVAALQNQSLVRCVANRSDGRSVLVSSTAK
                     GRATLQKGLARTVQQVAELLGRLDAAELEAMADAIKQARESKPPP"
     gene            complement(2128..3009)
                     /locus_tag="VM692_01875"
     CDS             complement(2128..3009)
                     /locus_tag="VM692_01875"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ50938.1"
                     /translation="MKVGFVGLGRMGQGMARRLLEAGHELSVFDQYAPQAASLKAAGA
                     HAAASVAEVAERSEIVVTMLVEDSAVSEVTLGARGLCETQPKGAIHLVMGTHGVAVVR
                     QLEARHRAAGQTLVAAPVLGRPDLAASGQLGIVVAGPQEAVARCTELLKSLGRRIFEA
                     GAKPESATAIKLANNAVLGCAMVAMAEGFALVRRYDVRPQVFQDVMVEGLFGATAYKV
                     YGQKMVDESFDQVGSPITVGLKDANLIAAAANLARVPMPSHNVYLDRLLGAVAHGDGD
                     RDQAVLAREQARASGIE"
     gene            3252..6557
                     /locus_tag="VM692_01880"
     CDS             3252..6557
                     /locus_tag="VM692_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HUQ50939.1"
                     /translation="MKKPPQVTLRFGVLASAVSIALSGGVTPVAAQDQVEELVVTGSR
                     IVRRDLEANSPIVTLDATRLEETSSVAIESVMNQLPQFVPAVSQFDAGNTFAGSQRTP
                     GAATLSLRGLGANRNLVLLDGRRAMPINASMAVSINTIPAAAIARVETITGGASSVYG
                     ADAVAGVVNFITKKDFEGFDLDFQTGETQEGDGAEHRFSGVFGANLADGRGNVLLGFE
                     RSIRNEIRRVDREFYREGFSDPYSNSAGSFYSAAGFTANAANLPSQAAVNAIFRDAAH
                     PTITTGRTGTFYMNPDGTMYKTAIDGTHRYNGGLLEPGTGLPWRKLDPITGTLRENQP
                     RTLAQIPLERSSLFGRATFEVSDNIRAFAQMMYSENSTRATGSDPPMLGGWRTPIPHG
                     NGIYGPSVQNLGADGLPNTGDAGENMATRINFMAGGVHGLNCAPVGGCTKSQVWPTPP
                     QLTALLNSRTLTPLAGQPAVPGSGAEADFDVVQSVVWAGSRRSFVDTLSYQLVGGLEG
                     EFGIKDWTWEAYVSNGSTRTINTYLGATSLARWRFVQQQPNYGKGLFYTGNEYGGGFG
                     AGTITCTSGIVSVYGVNGWTEGSVPSEDCQKAVTMQPQSVGHMKQDVAEYNMQGSILE
                     MPRRGDLRFAAGLSARTNKYQFYPDPTNTPQSVLDQPGSFFPVGEALGKTEAQELYGE
                     LLVPLLSDKPGAQSLSLEIGYRATDNEPSEDVDTYKMLVDWAIVDRVRFRGGRQIANR
                     APNIGELFQASEQFAPFTFVQGDPCSTRDPAQIPYTANPTINGARAARVQALCSALMG
                     PQGAATFYGDPSLQPNGLQVARISNLIGNPLLDSEKAETITAGFVIDVAERSNLTIDY
                     WRIRITDMIADEVGDILYENCMDEDTNPSYDPTHPACLRLVRNPSNGTNATLSTTFNN
                     QQQVDLAGYDVQFNWSTDIGPGVLSVSAQATIADHTKTRPSPTADWFDYKGSSGPSNI
                     RSVNSYSYDYRLLNSVSYSMGNWNGSLRWRYLPSLESEAAVRTTASFDEPTGAYSIFD
                     GSARYSLSSKTELRFGVDNLFNVDPEITFRESAHIDFNNQLGPGYTNSGDTNENFYDF
                     LGRRWYLGFKMSF"
BASE COUNT         1209 a         2148 c         2097 g         1105 t
ORIGIN      
        1 cgcagccgaa gcgcgccagc gcgtcgagcg tggctcgatt ctcgacgcct tcggcgcaga
       61 ccgtgaggtt gagcttgtgc gccagcgaca cgagcgcgtc gaccatgatc gacgcctctt
      121 tcgactcgcc gattttcgcg accaacgatc ggtcgatctt catctcgttg aacggcatgc
      181 ggaacagctg cgtcagcgac gaatagccga tgccgaagtc gtcgatcgca aggccgatgt
      241 tcttgatgcg caagcgcgtc aagatgtcga acgcttgcgg cgtctgctcg agcatcgccg
      301 tctcggtgat ctcgagcgcc agcgattccg cgtcgaccga ctgctcgacg agcatcgttt
      361 cgaggcgatc ggggaagccg atgtcggaga tcaacgtcgc ggcgatgttg acccgaagtc
      421 ctatgcgcaa gcgctgcgtt tcccaacctt tcagctgctc gatgccgcgt tgcaggacga
      481 agtccgtcat cgggcggctc aagccgtgag cttcacccag cgacacgaac gaatcgggag
      541 tcagcagacc gcggatcgga tgatcccaac gcagcagggc ctcgacggcc gcgatgtccc
      601 acgtgttgtc ggcgaaccgg cggatgatcg gctgatagtg caccagcaac tgttcgcgct
      661 cgatggcctg ctcgagatcg gcggccgtca ggggccggat cgcggcgtgc gccttctcga
      721 gccccgcgaa cagctcgtcc gggtcgaatg gcttttgcag cgtcccgaag acggcaagcc
      781 cgcggcgcag gccgtattgc tcggccgatg cgatcgtgcg ctcgtccatg cccgtcacca
      841 ggaagatttt cgccgcgcat tcgacgtcgg cgagatgccg cagcgcgctg acgccgtcct
      901 cgccgggcat ctgtaggtcg agaatgatga cgtcggggcg ctcgccggcg acaagctcgc
      961 gcaaacgcgc cgctgacgtc gaaggaatcg cgcggaaccc ggctcgagcg ccgatcgcgc
     1021 aaatggcctc gacgatgtct tgatcgtcgt cgagcgcgag caagacgggg tcgcggctag
     1081 gcacgctcaa ccttggccgg cgcgggcgac cgatggctgc gcgccgcgct cgccgtccgc
     1141 ggcactctcg ctgcacggca cttccgtgcg catgatcttg agcgcggccg gcgcctcgat
     1201 gacgagccgg acacgcttct gaccgacgct ctgtagccgg accgtgatgg cgccgccggc
     1261 gaaggcttcg cgcagcgtga gggcaggatc gagcccagcg acgggttcga tcctgatcga
     1321 ttcgttttcc ttgcgtgata cgacgagcac tcaatgctct cccgctcggg aatccgtccc
     1381 tgagcggctg caggattaca aaatgcgcag ccgcagaacg ccacctacgt cacaaaacca
     1441 aatgttttct tgcgcaatgc aaataaagtg cgtgcccagc gtacgttgtt tacggtcgat
     1501 cgagcgatgt cggtgcccgt cattcgacgc cgggaagcac gatcgcatcg aggtctgaac
     1561 cttctcaaca atggacggcg cgtgtagact cgcggccgac cgagaggcga tccgcgtcgc
     1621 cgtctcggcg cgcaagcaac aacgacgaga agggcactgt gactaccagc gatggcggat
     1681 ccgccccggc cgcagacgaa gctctgcgtg cagttctggc gttttatcgg ttgtcccgaa
     1741 gcctgcgcaa caccgacttg cgcgacctga cctgggagcg cttgagcacc cttagcgtcg
     1801 ttgcgcggcg cgaacccgtc tcgatctccg agctctcggt cgttgaggaa gtcacggcac
     1861 cgacgatctc gcggacagtc gcggcgctgc agaaccaatc gctagtgcgc tgcgttgcga
     1921 atcgcagcga cggtcgatcc gtgctcgtca gcagcactgc gaaggggcgc gcaacgctgc
     1981 agaaaggatt ggcgcgcacg gtgcaacagg tcgccgagct gctgggtcga ctcgatgcgg
     2041 ccgagctcga ggcaatggcc gacgcaatca agcaagcgcg cgagtcgaag cccccgccct
     2101 gagcccgcgc ctcgcgacgc ctacgcgtta ttcgatgccc gatgcgcgcg cctgctcgcg
     2161 cgcgagcacc gcctgatcgc gatcgccatc gccgtgcgcc acggcgccga gcaagcgatc
     2221 gagatacacg ttgtggctcg gcatcggcac gcgagccagg ttggcggccg cggcgatcag
     2281 attcgcgtct ttgagcccga cggtaatcgg cgagccgact tgatcgaagc tctcgtcgac
     2341 catcttttgg ccgtagacct tgtacgcggt cgccccgaac aagccttcga ccatgacgtc
     2401 ctggaacact tgcggtcgca cgtcgtacct gcgcacgagt gcgaagcctt cggccatcgc
     2461 gaccatcgcg cacccgagca ccgcgttgtt ggcgagcttg atcgcagtcg ccgactccgg
     2521 cttcgcgccc gcttcgaaga tccgccgacc gagcgacttg agcagctccg tgcaacgagc
     2581 aaccgcttct tgcggccccg cgacgacgat gccgagctga ccactggccg cgaggtcggg
     2641 ccggccgagc acgggcgctg caacgagcgt ctgaccggcc gcgcggtgcc gcgcttcgag
     2701 ctggcgcacg acggcgacgc catgcgttcc catgacgagg tggatcgcgc ctttcggctg
     2761 cgtctcgcaa agcccgcgcg cgccgagcgt aacttcgctg accgccgaat cctcgacgag
     2821 catcgtgacg acgatctcgc tgcgctcggc gacttcggcg accgatgccg ctgcatgagc
     2881 ccccgcggcc ttcaaggaag cggcctgcgg cgcgtactga tcgaataccg aaagctcgtg
     2941 accggcctcg agcaggcgtc gcgccattcc ttgtcccatg cggccgaggc caacgaaacc
     3001 gactttcatg ttttcatcct taaagagagc gacaaggaga gcgaccccaa aacgagcgcg
     3061 ggactgtgac ccgccggcca tgcagttgca agttgcgcgg acgcaaccag gccgctcgtt
     3121 cgcccctggt ggaccaaata ggggtaacag ccacggacct gtttgcaacc taggcggttt
     3181 gctctaatcg gtcggggctg tgtcgcatcg gcgacacgca tctcgggggc gaaacaagag
     3241 ggggagtgga cgtgaaaaaa ccaccacagg tcacgctgcg tttcggtgtt ttggcgagcg
     3301 cagtttccat cgcgctatcg ggcggcgtca cacctgtcgc ggcgcaagat caagtcgaag
     3361 agctcgtcgt caccgggtcg cgcatcgtca gacgtgactt ggaggccaac agccccatcg
     3421 tgacgctgga tgcgacgcgg ctcgaggaga cgagctctgt cgccatcgag tccgtcatga
     3481 atcaattgcc gcagttcgtg ccggcggttt cgcagttcga cgcaggcaac acgtttgccg
     3541 gatcgcagcg aactcccggt gccgcgacgt tgagcttgcg cgggctcggc gcgaaccgga
     3601 acctcgtgct tctcgacggc cgccgcgcga tgccgatcaa cgcctcgatg gcggtcagca
     3661 tcaacacgat tccggccgca gccatcgcac gcgtcgagac catcacgggc ggtgcgtcat
     3721 ccgtgtacgg cgccgatgcc gtcgcgggcg tcgtaaattt catcacgaag aaagacttcg
     3781 aaggcttcga cctcgacttc cagaccggcg agacgcagga gggtgacggc gcggagcacc
     3841 gcttctccgg cgtcttcggc gcaaacctcg ccgacggccg cggcaacgtg ttgctcggct
     3901 tcgagcggtc gatccgcaac gagatccgca gggtcgatcg cgagttctat cgcgaagggt
     3961 tcagcgaccc gtactcgaac tcggccggct cgttctattc cgcggccgga tttacggcca
     4021 acgcggcgaa tttgccctcg caggcggcgg tcaacgcgat cttccgcgat gcggcgcacc
     4081 cgaccatcac gaccgggcgg acgggtacgt tctacatgaa ccccgacggc acgatgtaca
     4141 agacggccat cgacggaacc catcgataca acggcgggtt actcgagccc ggcaccggac
     4201 tgccatggcg caagctcgac ccgatcacgg gtacgttgcg cgagaaccaa ccgcgcacgc
     4261 ttgcacagat tccgctcgag cgttcctcgc tgttcggccg cgccactttc gaggtgtcgg
     4321 acaacatcag ggcattcgcg cagatgatgt acagcgaaaa ctcgacgcgc gcgacgggct
     4381 cggatccgcc gatgctcggc ggttggcgca cgccgattcc gcatggcaac ggtatctacg
     4441 gtccgtccgt ccagaatttg ggcgcggacg gcttgccgaa caccggcgac gccggcgaga
     4501 acatggccac gcgcatcaac ttcatggccg gaggcgtgca cggcttgaac tgcgcccccg
     4561 tcggcggctg cacgaagtcg caagtgtggc caacgccgcc gcagctgacg gcgttgctga
     4621 acagccggac actgaccccg ctcgccggcc agccggctgt gcccgggtcg ggtgcggagg
     4681 ccgatttcga cgtcgtgcaa tcggtcgtgt gggccggctc gcggcgcagc ttcgtcgaca
     4741 cgttgtccta tcagctcgtg ggcgggctcg agggcgaatt tggaatcaag gactggacgt
     4801 gggaggccta cgtctcgaac ggctctacgc gcacgatcaa cacctacctg ggcgccacgt
     4861 cgttggctcg ctggcgcttc gtgcagcagc agccgaacta tggcaagggg ctgttctaca
     4921 cgggcaatga gtacggcggc ggcttcggcg ccggcacgat cacctgcacg tcgggcatcg
     4981 tctcggtcta cggcgtcaac ggttggaccg aaggctccgt gccgtcggaa gactgccaaa
     5041 aagccgtgac gatgcagccg cagtcggtcg gccacatgaa gcaggacgtc gccgagtaca
     5101 acatgcaggg ctcgattctc gagatgccgc gccgcggcga cctgcgcttt gcggccggcc
     5161 tctcggcgcg taccaacaag tatcagtttt accccgatcc gacgaacacg ccgcagtccg
     5221 tgctcgatca gccggggtcg ttcttcccgg tcggcgaggc cctcggcaag acggaggcac
     5281 aggagctcta cggcgagttg ctcgtgccgc tgctgtcgga caagccgggc gcacagagcc
     5341 tgagtctcga aatcggctac cgcgccacgg acaacgagcc cagcgaagac gtcgacacgt
     5401 acaagatgtt ggtcgattgg gcgatcgtcg atcgcgtgcg gttccgcggc ggccggcaga
     5461 tcgcgaaccg cgcgccgaac atcggcgagc tgttccaggc ttccgagcag ttcgcgccgt
     5521 tcacgttcgt gcaaggcgac ccatgctcga cgcgcgatcc cgcacaaata ccgtacacgg
     5581 cgaaccccac catcaacggg gcgcgcgccg cgcgcgtgca ggcgttgtgc tcggcgttga
     5641 tggggccgca aggagcggcg acgttctacg gcgacccgtc gctgcagccg aacgggctcc
     5701 aggtcgcgcg catctcgaac ttgatcggca atccgctgct ggactccgaa aaggccgaaa
     5761 ccatcacggc cggtttcgtg atcgacgtgg ccgagcgctc gaacctcacg atcgactact
     5821 ggcgaatccg catcacggac atgatcgcgg acgaggttgg cgacatcctc tacgagaact
     5881 gcatggacga ggatacgaac cccagctacg atccgaccca ccccgcgtgc ctgcggctcg
     5941 tgcgtaatcc gagcaacggc accaacgcga cgctgagcac gacgttcaac aaccagcagc
     6001 aagtcgacct cgcgggttat gacgtgcaat tcaactggag cacggacatc gggcccggcg
     6061 tgctgtcggt gagcgcccag gccacgatcg cggaccacac gaagacgcgg ccgtcgccga
     6121 cggcggattg gttcgactac aagggctcgt cggggccgag caacattcgc agcgtgaact
     6181 cgtactcgta cgactaccgc ttgttgaatt cggttagtta ctcgatgggc aactggaacg
     6241 gcagcctccg ctggcgatac ttgccgagcc tcgagagcga ggctgccgtg cgcacgacgg
     6301 cctcgttcga cgagccgaca ggcgcctaca gcattttcga cggctcggcg cggtatagct
     6361 tgtcgagcaa gacggagctg cgcttcggtg tcgacaactt gttcaacgtc gatcccgaaa
     6421 ttacgttccg ggaatcggcc catatcgact tcaacaatca gcttgggccc ggctacacga
     6481 acagcggcga caccaacgag aacttctacg acttcctcgg tagacgttgg tacctcggct
     6541 tcaagatgag cttctgaac
//
LOCUS       DATLEE010000088         2949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3033163, whole genome shotgun sequence.
ACCESSION   DATLEE010000088 DATLEE010000000
VERSION     DATLEE010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3033163"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VM692_01885"
     CDS             complement(<1..987)
                     /locus_tag="VM692_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HUQ50940.1"
                     /translation="MTANLTPADAAVRARSVERRHAALRQWLAANDAEALIAYGSGHH
                     AFTGTNPAWYASAYKQLGPHMAVLLPREREPLLILTPTWDAARYRERATMEFVAVPPE
                     DFLTTVGHEIKKRALRGKRLAVAGGNLQPKAIATAWPHELGAAPLTAEKLISDTAKIR
                     DEWSLNCTRRGVAIAERGYQNLLETTTVGMREYELAANLEVFMRSLGGEDNFQLMSAS
                     QHNTAGHFPTDRVLQKGDLVLAEITPAVEGEFIQICRSAVLGGPSQLQLEKFALLTHA
                     LEAGMRAAKPGVSVTEIVDVINKPIAAAGYEQYTKPPYMRTRGHSMGLGSMEP"
     gene            complement(984..2759)
                     /locus_tag="VM692_01890"
     CDS             complement(984..2759)
                     /locus_tag="VM692_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HUQ50941.1"
                     /translation="MSAIQEPSAQHLDPVTLEVIRNALPAISDEMSVDLQRTSYNMMI
                     YEVRDYCTALLDVDGALISQNIGGVSHFVADLGVVVKDAVARYGREGFAPGDVLLHNH
                     QATAGQHLNNVVVYSPVFYRGELVAFAMVRAHWVDVGGLSTGFGAGNRAYDPWSEGLQ
                     FNQLKIYEGGVVDKKMLAFIRDNIRFPDNAMGDMRSQLAACRLGERRFVELLERYGKD
                     VVQASIRQFYAETEQKCRAAVAAIPDGIYEAESFIDWRGKGYDIKVKVVVSGSDMTID
                     LSGCSPEREGGANSRTFAGAYIAYKALTTPTEPLNEGAFSALKVIIPEGNMMMARFPA
                     FMAGWSMPLPTVVDTILLALAKAVPDQIPAAHSGSLGAALSYSGRDRVRNRDFVLMSI
                     ESGGWGGRGDADGEDVSMSVCQGDVRNAPIENLELKGPVIVEERALRPDSGGAGKYRG
                     GLGVRTRIRSLADGRINVQGGDGGRLTCPPWGLVGGGSGDVAKTLVKAPGEKEFHAPT
                     AQPLNWPAGTEVLYMTAGGGGWGDPLERDAERVLRDVREEYITEAHARERYGVVLTAD
                     RTAVDAAATAALRRDLRARQPKGTK"
     gene            complement(2756..>2949)
                     /locus_tag="VM692_01895"
     CDS             complement(2756..>2949)
                     /locus_tag="VM692_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50942.1"
                     /translation="YLDSAVQPTSCAVYQRERLAPNMTIEGPAVIEEYASTTVLFPGD
                     RVRVAETGELIVTVRKGEA"
BASE COUNT          419 a         1028 c          995 g          507 t
ORIGIN      
        1 gggctccatc gagcccaagc ccatgctgtg gccgcgcgtg cgcatgtacg gcggcttcgt
       61 gtactgctcg tagcccgcgg ccgcgatcgg cttgttgatg acgtcgacga tctccgtcac
      121 cgagacgccg ggcttcgcgg cccgcatgcc ggcctcgagc gcgtgcgtca gcagcgcgaa
      181 cttctcgagc tgcagctgcg acgggccgcc gagcacggcg ctgcggcaaa tctggatgaa
      241 ctcgccttcg acggccggcg tgatctccgc caacacgagg tcgcccttct gcagcacgcg
      301 gtccgtcggg aagtgcccgg ccgtgttgtg ctgcgacgcc gacatcagct ggaagttgtc
      361 ctcgccgccc aagctgcgca tgaacacctc gaggttcgcc gcgagctcgt actcgcgcat
      421 gccgaccgtc gtcgtctcga gcaagttctg atagccgcgc tcggcgatgg cgacgccgcg
      481 gcgcgtgcaa ttcagcgacc actcgtcgcg gatcttcgcc gtgtcgctga tcaacttttc
      541 ggcagtcagc ggcgccgcgc caagctcatg cggccatgct gtggcgatcg ctttcggctg
      601 caggttgccg cctgcaacgg cgagccgctt gccgcgcagc gcgcgcttct tgatctcgtg
      661 gccgaccgtc gtgagaaagt cctcgggcgg cacggcgacg aattccatgg tcgcgcgctc
      721 gcgatagcgc gccgcgtccc acgtcggcgt caggatcagc agcggctcgc gctcgcgcgg
      781 caacagcacg gccatgtggg ggccgagctg cttgtaggcg ctcgcgtacc acgccgggtt
      841 cgtgcccgtg aacgcgtggt ggcccgagcc gtaggcgatg agcgcctcgg cgtcgttggc
      901 ggcgagccat tggcgcagcg cggcatggcg gcgctcgacg ctgcgcgcgc gcacggcggc
      961 atcggccggt gtcagattcg ctgtcatttc gttcccttgg gttggcgcgc gcgcaggtcg
     1021 cggcgcagcg cagccgttgc cgccgcgtcg accgcggtgc gatccgccgt cagcactacg
     1081 ccgtatcgct ctctggcgtg cgcctccgtg atgtactcct cgcgcacgtc gcgcagcacg
     1141 cgctcggcat cgcgctcgag cggatcgccc cagcctccgc cgccggccgt catgtacagc
     1201 acctcggtgc cggcgggcca gttcagcggt tgcgcggtcg gcgcatggaa ttctttctcg
     1261 ccgggcgctt tgactagcgt cttggcgacg tcgccgctgc cgccgccgac gagcccccac
     1321 ggcgggcacg tcaaccgccc gccgtcgccg ccctgcacgt tgatgcggcc gtcggccagc
     1381 gaacgaatgc gcgtgcgtac gccgagaccg ccgcgatact tgccggcgcc gccggagtcg
     1441 ggccgcaacg cgcgctcctc gacaatgacg gggcctttga gctcgaggtt ctctatgggc
     1501 gcattgcgca cgtcgccttg gcagacggac atcgagacgt cctcgccgtc ggcgtcgccg
     1561 cgcccgcccc agccgcccga ctcgatgctc atcagcacga agtcgcggtt gcgcacgcgg
     1621 tcgcggccgg agtacgacag cgccgcgccc agcgagccgc tgtgcgcggc cggaatttgg
     1681 tccggcacgg ctttcgcgag cgcgagcagg atcgtgtcga cgacggtcgg caacggcatg
     1741 ctccagcccg ccatgaacgc ggggaagcgc gccatcatca tgttgccttc ggggatgatg
     1801 accttcagcg ccgagaacgc gccttcattg agcggctccg tcggcgtcgt tagcgccttg
     1861 tacgcgatgt acgcgcccgc gaacgtgcgc gagttggcgc cgccttcgcg ctccggcgag
     1921 cagcccgaga gatcgatcgt catgtcgctg ccgctgacga cgactttgac cttgatgtcg
     1981 tagcccttgc cgcgccagtc gatgaagctc tcggcctcgt agataccgtc gggtatcgcc
     2041 gcgacggccg cgcggcactt ctgctccgtc tcggcataga actggcggat cgacgcctgc
     2101 acgacgtcct tgccgtagcg ctcgagcagc tcgacgaacc gccgctcgcc gagccggcac
     2161 gccgcgagct gcgaacgcat gtcgcccatc gcgttgtcgg ggaaacggat gttgtcgcgg
     2221 atgaacgcga gcatcttctt gtcgacgacg ccgccttcgt agattttgag ctggttgaac
     2281 tgcagccctt cgctccaggg gtcgtaggcg cgattgccgg cgccgaaacc cgtcgagagc
     2341 ccgccgacgt cgacccagtg cgcgcgcacc atcgcgaatg cgacgagctc gccgcggtag
     2401 aacaccggcg agtagacgac gacgttgttc aagtgctggc cggccgtcgc ctgatggttg
     2461 tgcagcagca cgtcgccggg cgcgaagcct tcgcggccgt agcgcgcgac ggcgtccttg
     2521 acgacgacgc cgagatccgc gacgaagtgc gagacgccgc cgatgttctg cgagatcaac
     2581 gcgccgtcga cgtcgagcag cgccgtgcaa tagtcgcgca cctcgtaaat catcatgttg
     2641 tacgacgtgc gctgtaagtc gacgctcatc tcgtcggaga tcgcgggcag cgcgttgcgg
     2701 atgacctcga gcgtgacggg gtccaagtgc tgcgcgctcg gctcttgaat cgcgctcatg
     2761 cttcaccctt ccgcaccgtg acgatgagct cgccggtctc ggcgacgcgc acgcggtccc
     2821 cggggaacag caccgtcgtc gacgcgtatt cctcgatcac ggccgggcct tcgatcgtca
     2881 tgttgggcgc gagccgctcg cgctggtaga cggcgcagct cgtcggctgc acggcactgt
     2941 cgagataga
//
LOCUS       DATLEE010000089         3241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_104232, whole genome shotgun sequence.
ACCESSION   DATLEE010000089 DATLEE010000000
VERSION     DATLEE010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104232"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VM692_01900"
     CDS             complement(<1..540)
                     /locus_tag="VM692_01900"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HUQ50943.1"
                     /translation="MSRGSSELALATSAPFHLEATVRVLQRRPTNPVDVWRDACWERV
                     FKTADGLVLSVVENRGSIDSPDLRFAIRAGSPSTATLASLEQTLRQMLNLDLDPALLA
                     AAAHRLPALRATAAALRGMRPPRFVDLFEVFLNVVPFQQLSIDAGAAILGRLVERFGA
                     ELAHGERVYRAFPSAAAIAG"
     gene            complement(537..938)
                     /locus_tag="VM692_01905"
     CDS             complement(537..938)
                     /locus_tag="VM692_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUQ50944.1"
                     /translation="MDVQVDGNLIRAKREQRAWSQEHLAAAAGIGARTIQRIEATGVA
                     SYESVRAISAALETPISALSLAAEPGPAPRLRLAARWLGAAVALAFFAGASGFLLMKD
                     YKKVRVLRTLEQWVTTDAGPTTGAANRTDGG"
     gene            1135..1854
                     /locus_tag="VM692_01910"
     CDS             1135..1854
                     /locus_tag="VM692_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50945.1"
                     /translation="MRLIPLIPAALIVAAPLFAQDWETVTVAEDGFRSNFPGQPTVEA
                     IRYETEFRMALPGHVYRAADALGRYSTTVVDYRDAQRLHDERSAKCLAAKGANQQDGD
                     TCQNDFVMEVAGAMDFAAAKFLTRDGAKVTQYGTYFLDMVSGRLIQLTNADRSRTYAA
                     IHQHAGRLYIHEATVPADMPEPILFLQGLAWVDDTGQAIRYRTLYTEGYADWKFPQPT
                     PPPHTLRDLDASREQPPRPPR"
     gene            1865..2254
                     /locus_tag="VM692_01915"
     CDS             1865..2254
                     /locus_tag="VM692_01915"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ50946.1"
                     /translation="MPWKHLAAASVALATSMAALAHHSHANYDVSKWTVVEGTVKQVV
                     LIAPHSIVYLDVKNDAGATATWALEATAPVGILNNGVKREDVRAGDPIQARCHRLRDG
                     ANGCLLGFITPLHGDAARGHGIEREWD"
     gene            complement(2227..>3241)
                     /locus_tag="VM692_01920"
     CDS             complement(2227..>3241)
                     /locus_tag="VM692_01920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03885 family FMN-dependent LLM class
                     oxidoreductase"
                     /protein_id="HUQ50947.1"
                     /translation="VISFSYHASHEQFPPSELLRYVRQAEAAGFDAAFSSDHLQPWSQ
                     AQGHSGFAWAWLGAALHSTERMRFAAITVPGGWRYQPVVLAQAIATLGEMFPGRFPWV
                     ALGSGEALNERATAGRWPSKSSRHARLQRGAEIISELLDGKTVTRAGPPSAHEARIWS
                     LPAERTALVGAAVTAATAAWLGSWADGLLTTAGTLEELRANVEAFRRNGGEGKPVYVK
                     TQVCWAETDEQASRFAFEQWHCNAVPRERCAELAQPAHFEHAARDISTRDVERVVCVS
                     SDLERHTAWLRDIAALGVDGIDIHQVGRNQAEFIAAFGRHVLPALRYAPAPRAQSHSR
                     SMP"
BASE COUNT          553 a         1130 c         1086 g          472 t
ORIGIN      
        1 gcctgcaatc gccgcggccg acggaaacgc gcggtagacg cgctccccgt gcgcaagctc
       61 ggcgccgaag cgctcgacga gccggccgag gatcgcggcg cccgcgtcga tgctgagctg
      121 ctggaacggc acgacgttca gaaaaacctc gaacaggtcg acaaagcgcg gcggccgcat
      181 gccgcgcagc gcggccgccg tcgcgcgcag cgcgggcagc ctgtgcgccg cggcggcgag
      241 cagcgccgga tcgagatcga gattgagcat ctgccgtagc gtctgctcga ggctcgcgag
      301 cgtcgcggta gacggcgagc cggcccgaat cgcgaaccga agatccggcg agtcgatcga
      361 gccgcggttt tcgaccacgc tgagcacgag accgtcggcc gtcttgaaca cgcgctccca
      421 gcacgcgtcg cgccacacgt cgacggggtt cgtggggcgg cgttgcagca cgcggacggt
      481 cgcttcgaga tgaaacgggg ccgaagtcgc tagcgcgagc tcgctcgagc ctctactcaa
      541 ccgccgtctg ttcggttcgc agcgccggtc gtaggcccgg cgtcggttgt gacccactgc
      601 tcgagcgtgc gcagcacgcg taccttcttg taatccttca tcagcagaaa gccgctggcg
      661 ccggcgaaga atgcgagcgc cacggcggcg ccgagccagc gcgccgcaag gcgcaagcgc
      721 ggcgcgggtc ccggctctgc ggctaacgac aaggcgctga tcggcgtctc gagcgccgcg
      781 gaaattgccc gcaccgattc ataggatgcg acgccggtgg cctcgatgcg ctggatcgtc
      841 cgcgcaccga tgccggcggc tgccgccagg tgctcctggc tccacgcgcg ctgctctcgt
      901 ttggctcgaa tcaggttgcc gtcgacttgc acgtccatgc tctcgtccct cgcctgcgaa
      961 gacgaggatt catcgcagaa tacccgaacg ccggccacgc gatcagcacg ccacggccgc
     1021 gccacgggca cgccgcggcc gcgccgaaag agtcgacggt cgccggccag ttgccttcgg
     1081 ggcccggtct cggcaacatc ggtagcggtc cgagacggtc gaagtgggga agccatgcgc
     1141 ttgattccac tgattccggc agcgctcatc gtcgccgcgc cgctgtttgc acaggactgg
     1201 gagacggtca ccgtcgccga ggacgggttc cgttccaact tcccgggcca gccgacggtc
     1261 gaggccatcc gctacgagac cgagttccgc atggcgctgc cgggccacgt ctatcgcgcg
     1321 gccgacgcgc tcggccgcta ctcgacgacc gtcgtcgact atcgcgatgc ccagcgcctg
     1381 cacgacgagc gctcggcgaa gtgcctcgcc gccaaaggcg cgaaccagca agacggcgac
     1441 acgtgtcaga acgacttcgt catggaagtt gccggcgcga tggactttgc cgccgcgaag
     1501 ttcttgacgc gcgacggcgc gaaggtcacg cagtacggca cgtacttcct cgacatggtg
     1561 tcggggcggc tcatccaatt gacgaatgct gatcgctcgc gcacgtacgc ggcgattcat
     1621 cagcacgccg gccggttgta catccacgag gcaacggtgc ctgcggacat gccggagccg
     1681 atcctgttcc tgcaggggct cgcgtgggtc gacgacacgg gccaagccat tcgctatcgc
     1741 acgctgtata cggaaggcta tgcggactgg aaattcccgc agcccactcc accgccccat
     1801 acgttgcgcg acctcgacgc gtcgcgtgag caaccgccgc gcccgccacg ctaggagacg
     1861 cacgatgccg tggaaacacc ttgcggccgc ctctgtcgca ctcgccacga gcatggccgc
     1921 gctcgcgcat cactcgcacg cgaactacga cgtctcgaag tggaccgtcg tcgaaggcac
     1981 ggtcaagcaa gtcgtgctga tcgcgccgca ctcgatcgtc tacctcgacg tcaaaaacga
     2041 cgccggcgcg acggcgacgt gggcgctcga ggcgacggcg cccgtcggca tcctcaacaa
     2101 cggcgtcaaa cgcgaggacg tgcgggccgg cgatccgatc caagcgcgtt gccataggct
     2161 cagagacggc gcgaacggct gcctgctcgg cttcatcacg ccgctgcacg gcgacgcggc
     2221 gcgcggtcac ggcatcgaac gcgagtggga ctgagcacgc ggcgccggcg catagcgcag
     2281 cgcgggcaac acgtggcggc cgaacgccgc gatgaactcg gcctggttcc gcccgacttg
     2341 atgaatatcg atgccgtcga cgccgagcgc tgcgatgtcg cgcagccagg ccgtatggcg
     2401 ctcgagatcg ctcgaaacgc agacgacgcg ctcgacgtcg cgcgttgaaa tgtcgcgcgc
     2461 cgcgtgctcg aaatgcgcgg gttgcgcgag ctcggcgcac cgctcgcgcg ggaccgcgtt
     2521 gcaatgccac tgctcgaacg cgaatcgcga tgcctgctcg tcggtctcgg cccagcagac
     2581 ttgcgtcttc acatagacgg gcttgccctc gccgccattg cggcggaacg cttccacgtt
     2641 ggcgcgcaac tcctcgagcg tcccggccgt cgtcaacaga ccgtcggccc atgacccgag
     2701 ccacgccgcg gtcgcagccg tcacggcggc accgacgaga gcggtccgct ccgccggcag
     2761 cgaccaaatg cgcgcttcgt gcgcgctcgg cggtccggcg cgcgtgacgg tttttccgtc
     2821 cagcaattcg ctgatgatct cggcgccgcg ttgcagacga gcgtgacggc tcgacttact
     2881 gggccagcgt cccgccgtcg cacgctcgtt gagcgcttca ccgctgccga gcgcgaccca
     2941 agggaagcgg ccgggaaaca tctcgccgag cgtcgcaatc gcttgcgcca acacgaccgg
     3001 ttgatagcgc cagccgccgg gcacagtgat cgcggcaaag cgcatgcgct cagtcgaatg
     3061 gagcgctgcg ccgagccagg cccaagcgaa accggaatgc ccctgcgctt gcgaccaggg
     3121 ctgcagatgg tccgacgaaa aggcggcgtc gaagccggcc gcctcggcct ggcgcacgta
     3181 gcgtaggagc tcgctcggag gaaactgctc gtgcgaggcg tgatagctga acgagatcac
     3241 g
//
LOCUS       DATLEE010000090         2688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3033774, whole genome shotgun sequence.
ACCESSION   DATLEE010000090 DATLEE010000000
VERSION     DATLEE010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3033774"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VM692_01925"
     CDS             complement(<1..307)
                     /locus_tag="VM692_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HUQ50948.1"
                     /translation="MEAQPLLLALNAGSSSLKAKLYACTETKLTLESSARLDGIGRRQ
                     ARLRVDDVTFSGGAVGDHARAAEVLLDRLLPGASTTADRLVAIGHRIVHGGAQFTRPV
                     "
     gene            563..1240
                     /locus_tag="VM692_01930"
     CDS             563..1240
                     /locus_tag="VM692_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ50949.1"
                     /translation="MIRLNNVEKSYPYKGGQTWVLRQIGIEIAEGDFVTIMGPSGSGK
                     STLLAILGMLDGEYQGEYWFDEKALHKASVKERAKVNKGNVGFVFQHFHLLDDLTVYE
                     NLEIPLSYRDVPRKEREARVAEVLDRFQMVGKKDLYPTQLSGGQQQQVAVARAVIAKP
                     RVVLADEPTGSLHWSQGEEIMRLLKGLNEHGTTIVQVTHDERVAGFGRRLIELGDGWI
                     TKDRNLA"
     gene            1307..2605
                     /locus_tag="VM692_01935"
     CDS             1307..2605
                     /locus_tag="VM692_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUQ50950.1"
                     /translation="MRLLLKGEGYSAETFVNPEGLVSALRERTADAVLMDLNYTRDTT
                     SGDEGLDTVSRIRSFDAHTPIVLMTAWGTINLAVEGMRRGAQDFVEKPWDNDRLLTVL
                     RTHVALGRALQRARTLEAENRQLKGTGGEALIAESAAMQPVLETIERVAPTDANVLIT
                     GESGCGKGLVARLIHDASPRRERSLVTVNIGSLAETVFESEMFGHVKGAFTDAKSDRV
                     GRFELADGGTLFLDEIANIPVAQQGRLLRVLEDGQFERLGSSRTQRADVRVLAATNAD
                     IDAEIGAGRFRADLLYRLNTIHIQLPPLRERGDDIVLLAQRSLNQHAQHHRRPITSFP
                     DAALSALRRYPWPGNVRELNHVIERAVLMARGTAIEPADLRLTAAPASGDRKAMTLDE
                     VELDAIRAALARHDGNVIAAADELGMSRSALYRRMEKFGL"
BASE COUNT          468 a          875 c          914 g          431 t
ORIGIN      
        1 gcacgggtcg cgtgaattgc gcgccgccgt gaacgatgcg gtgcccgatg gcgacgagcc
       61 ggtccgccgt cgtgctcgcg cccggcaaca aacgatcgag cagcacctcg gcggctcgcg
      121 cgtggtcgcc gacggctccg ccggagaacg tgacgtcgtc gacgcgcagc cgcgcttgcc
      181 ggcggccgat gccgtccaga cgcgcgctcg attcgagcgt cagcttcgtt tcggtgcagg
      241 cgtagagctt cgccttcagc gacgagctgc ccgcattcaa cgcgagtaat agcggttgtg
      301 cttccatgca ccctcctcgg gcggacagtg caagccttgt gcctattggt ttcagacgtt
      361 gacggctctc gttcttgccc gcacgctcac gtttgtcccg aaaatgggac gcgagtcggt
      421 gcagccggga cccgacagcc acgtgcgcgc gcagtgacga gcggaaagcc gttgcggcac
      481 agcgagttgc gcgctccgtc acgcttggca tgggagttgc tctgccggct gcgagcacgt
      541 cgaccacaac aagaggcgcg agatgattcg tttgaacaac gtcgagaagt cgtatcccta
      601 caaaggcggg cagacctggg tgctgcggca gatcggcatc gagatcgccg aaggcgactt
      661 cgtgacgatc atgggcccgt cgggctcggg taagtcgacg ttgctcgcga tcttggggat
      721 gctcgacggc gagtaccaag gcgagtattg gttcgacgag aaggcgctgc acaaagcgag
      781 cgtcaaggaa cgcgccaagg tcaacaaagg aaacgtcggc ttcgtgttcc agcatttcca
      841 cttgctcgac gacttgacgg tctacgagaa cctcgagatc ccgctgtcgt accgcgacgt
      901 accgcgcaaa gagcgcgagg cgcgcgtcgc cgaggtcctc gatcgcttcc agatggtcgg
      961 caagaaagat ctctatccga cgcagctgtc gggcggtcag cagcaacagg tcgccgtcgc
     1021 gcgcgccgtc atcgcgaagc cgcgggtcgt gctcgccgac gagccgacgg gcagcctgca
     1081 ctggagccag ggcgaagaga tcatgaggct gctcaagggc ctcaacgagc acggcacgac
     1141 gatcgtgcag gtcacgcacg acgagcgcgt ggcgggcttc ggccggcggc tcatcgagct
     1201 cggcgacggc tggatcacca aagaccgcaa cctcgcctga gcgggcatgc caacccgtcc
     1261 accccgaatt ttcgtagctg acgatcagcg cgacgttctc gaatccttgc ggttgctgct
     1321 gaaaggcgag ggctattccg ccgagacgtt cgtgaacccc gaaggtctcg tgtcggcgct
     1381 gcgcgagcgc acggcggacg ccgtgctcat ggacttgaac tacacgcgcg acacgacgtc
     1441 cggcgacgaa ggcctcgaca ccgtgtcgag aattcgctcg ttcgacgcgc acacaccgat
     1501 cgtgctgatg acggcgtggg gcacgatcaa tctcgccgtc gaaggcatgc gtcgcggcgc
     1561 gcaagacttc gtcgagaagc cgtgggacaa cgaccgcctg ttgacggtgc tgcgcacgca
     1621 cgtcgcgctc gggcgcgcgc tacagcgcgc gcgcacgctc gaggccgaga accgccagct
     1681 gaaaggcaca ggcggtgaag ctttgatcgc ggagtccgcg gcgatgcagc ccgtgctcga
     1741 gacgatcgag cgcgtagcgc cgaccgacgc caacgtgctg atcacgggcg agagcggctg
     1801 cggcaaaggt ctcgtcgcgc gcttgattca cgacgcgtcg ccgcggcgcg agcgcagcct
     1861 cgtgaccgtc aacatcggca gcctcgccga gacggtgttc gagagcgaga tgttcggtca
     1921 cgtcaaaggc gcgttcacgg acgcgaagag cgatcgtgtc ggccggttcg agctcgccga
     1981 cggcggcacg ctgttcctgg acgaaatcgc gaacattccc gtcgcgcagc aaggcaggtt
     2041 gctgcgcgtg ctcgaggacg gccagttcga gcggctcggc tcttcgcgta cgcagcgcgc
     2101 cgacgtgcgc gtgctcgcgg cgacgaatgc cgacattgac gccgagatcg gcgccgggcg
     2161 gttccgcgcg gatttgctgt atcgcttgaa caccattcac atccaattgc cgccgctgcg
     2221 cgagcgcggc gacgacatcg tgctgctcgc gcagcggtcg ttgaaccagc acgcgcagca
     2281 tcatcggcgc ccgatcacgt cgttccccga cgccgcgttg tcggcgctgc gccgctaccc
     2341 ctggcccggc aacgtccgcg agctcaatca cgtcatcgag cgcgccgtgc tgatggctcg
     2401 cggcacggcg atcgagcccg cggatctgcg cttgacggcc gcgccggcga gcggcgatcg
     2461 taaggcgatg acgctcgacg aggtggagct cgacgcgatt cgcgcggcgc tcgcgcgcca
     2521 cgacggcaac gtcatcgcgg cggccgacga gctcggcatg agccgctcgg cgctgtatcg
     2581 gcgcatggag aagttcggtc tgtgaagcaa cgcaggcgcc tgcgtttcca aacccgagtg
     2641 ctggtcctcg tgctgctggc cgtcgcgccg gcctcgatcg cgacggtc
//
LOCUS       DATLEE010000091         3984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10486171, whole genome shotgun sequence.
ACCESSION   DATLEE010000091 DATLEE010000000
VERSION     DATLEE010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10486171"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..763)
                     /gene="radC"
                     /locus_tag="VM692_01940"
     CDS             complement(89..763)
                     /gene="radC"
                     /locus_tag="VM692_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HUQ50951.1"
                     /translation="MGQSHWPPSEQPRERLLEAGPQALSDGELLAILLGTGVRGQPVG
                     ELARRLLGESGGLTALLSRSPMELVRSKGLGPARVARLLAAVEIGRRYLGAGLPPRSA
                     LAAPGDAARFLKAKLLDLPHEVFCCLFLDTRHRLIRYEELFRGTIDGATVYPREVVKR
                     ALAHNASAVILGHNHPSGVSEPSEADRNITLKLAKALALVEIRLLDHVVVSRGGHVSL
                     AERGLV"
     gene            912..2156
                     /gene="coaBC"
                     /locus_tag="VM692_01945"
     CDS             912..2156
                     /gene="coaBC"
                     /locus_tag="VM692_01945"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HUQ50952.1"
                     /translation="MASTERASAPRVLLGVTGGIAAYKSPELVRRLVERGCEVQVVMT
                     RGAREFVGALTFQAVSGRRVRDDLWDPAAEAAMGHIELARWADVVLVAPATAHFLGTL
                     AAGFGGDLLSTVCLATTAPIVLAPAMNQAMWANAAVQANRALLEARGVRMLGPASGDQ
                     ACGETGPGRMLEPNEIAAALLEQTGQLRMQPLKGVKVVVTAGPTREPIDPVRYITNRS
                     SGKMGFAVAAAAREAGAEVVLVTGPVALPTPAAVRRIDVETAEQMYDSVHGEIAGAEI
                     FIGCAAVADYRPRAASEQKIKRSAAEMDLALVRSPDTLASIAALPQPPFTVGFAAETN
                     DVAAHARDKLDRKRIDMIAANQVGPDCGFDRETNALTVFWPGGGELALGEGSKGRLAR
                     RLVEVIAERYRARQRAKPSPAV"
     gene            2186..2653
                     /gene="dut"
                     /locus_tag="VM692_01950"
     CDS             2186..2653
                     /gene="dut"
                     /locus_tag="VM692_01950"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HUQ50953.1"
                     /translation="MTSTARRVELKILNPKIGKELPLPKRATAGSAGLDLRACLDAPL
                     DLSPGDTKLIPTGLAIHIADPQLAAVIIPRSGLGHKHGVVLGNLVGLIDSDYQGELMI
                     SCWNRGSQTFRVEPGERIAQLVVVPVVQVEFDVVEDFAASDRGSGGFGHTGRS"
     gene            complement(2654..3301)
                     /locus_tag="VM692_01955"
     CDS             complement(2654..3301)
                     /locus_tag="VM692_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HUQ50954.1"
                     /translation="MSRLGAILLVVAALVLPSTGSAQARDKDTRLYKWTDRNGVVHYG
                     DNIPPEYANLDRNVLNEQGVAVAFEEGEVTAEERAVLQERERVLAAERAAKAEIARRD
                     RMLLETYLSVSDIEDLRDRRLELLESQIKVTELYLGNLRKRLVGLQAEASSFKPYTTR
                     PDAPQVPENLALDIRRTTASISLYEQTLARTRSDQQALRLSFDGDIARFRELKGG"
     gene            complement(3409..>3984)
                     /gene="pyrE"
                     /locus_tag="VM692_01960"
     CDS             complement(3409..>3984)
                     /gene="pyrE"
                     /locus_tag="VM692_01960"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HUQ50955.1"
                     /translation="GRESPYFFNAGLFSDGQAAAVLGRCYAASLLASGIEFDVLFGPA
                     YKGIPLVATTAVALAEHHGRNVPFAFNRKEAKDHGEGGTIIGAPLAGRVLIVDDVITA
                     GTAVREVLDIVRAAGATPAGVLVALDREEVAPKARISAVQQLEGELQVPIRSIVTLTD
                     LIDHLEEDKEYEQHLPAVRAYRNRYGVPRES"
BASE COUNT          672 a         1327 c         1353 g          632 t
ORIGIN      
        1 aaaattcctg aaagccctcg aaggggcggg gactatacct gctggccgtg gggtcgggca
       61 aggcgcgcag ccgcactcgg ccactcgctc aaacgagccc gcgttcggcc agcgaaacgt
      121 ggccgccgcg gctcacgacg acgtggtcga gcagcctgat ctcgacgagg gccagagcct
      181 ttgcgagctt caacgtgatg ttacggtcgg cctcgctggg ctcggacacg cccgaagggt
      241 gattgtggcc aagtatcacg gcggacgcat tgtgcgcgag cgcccgcttg acgacctcgc
      301 gcgggtagac cgtcgcgccg tcgatcgtgc cgcggaacag ctcctcgtag cgaatgagcc
      361 ggtgccgcgt gtcgagaaac aggcaacaga agacctcgtg cggcaggtcg agcagcttcg
      421 ctttgagaaa tcgggccgcg tccccgggcg ccgccaacgc cgagcgcggc ggtagcccgg
      481 cgccgaggta gcggcggccg atctcgaccg cggcgagcaa gcgggccacg cgcgccggtc
      541 ccaagccctt gctgcgcacg agctccatgg gactgcggct gagcagcgcc gtgagcccgc
      601 ccgactcgcc gagcaggcgg cgcgcgagct cgccgacggg ttggccgcgc acgcctgtcc
      661 cgagcaggat cgcgagcagc tcgccgtccg agagcgcttg cggcccagcc tcgagcagcc
      721 gctcgcgggg ctgctccgac ggcggccagt ggctctgacc catgactttc tccgtaaagg
      781 atcggggtgc catcgtcgcg ccgctcgcgg ctgcgaggca gcccgaatag tccgcgaaga
      841 aataagaaaa gctcgattag ccagtggcac cgcgcgcgag cccaagagta agctcggccc
      901 cggagactcc catggcaagc acggaacgcg cctcggcgcc gcgagtgttg ctcggcgtga
      961 cgggcggcat cgcagcgtat aagagccctg agctcgttcg tcgcctcgtc gagcgcggct
     1021 gcgaagttca ggtcgtcatg actcgcggcg cacgcgagtt cgtcggtgcg ctgacgtttc
     1081 aagctgtttc gggtcgccgc gtccgcgacg atctttggga ccccgcggcc gaagccgcca
     1141 tgggccatat cgagctggcg cgttgggccg acgtcgtcct cgtcgcgccg gcgacggcgc
     1201 acttcctcgg cacgttggcg gccggcttcg gcggcgactt gttgtcgacg gtgtgcctcg
     1261 ccacgacggc gccgatcgtg ctcgcgccgg cgatgaacca agccatgtgg gcgaacgccg
     1321 ccgtgcaggc gaatcgagcg ctgctcgaag cgcgcggcgt ccggatgctc ggtcctgcga
     1381 gcggcgacca ggcatgcggc gagacggggc ccggacgaat gctcgagccc aacgagatcg
     1441 ccgcagcgtt gctcgagcaa acgggtcagc tgcgcatgca gccgctgaag ggcgtcaagg
     1501 tcgtcgtgac cgcgggcccg acgcgcgagc cgatagaccc cgtccgttac atcacgaatc
     1561 gcagctccgg aaagatgggc ttcgcagtcg cggcagcggc gcgtgaagca ggcgccgaag
     1621 tcgtgctcgt cacggggccc gtagcactgc cgacgcccgc ggccgtgcgc cgcatagacg
     1681 tcgagaccgc ggagcagatg tacgacagcg ttcacggcga gatcgccggc gccgagattt
     1741 tcatcggttg cgccgcagtt gcggactatc ggccgcgcgc agcgtcggag cagaagatca
     1801 agcgcagcgc cgccgagatg gacctcgcgc tcgtgcgctc gccggacacg ttagcgagca
     1861 tcgcggcact gccgcagccg ccgttcaccg tgggcttcgc cgccgagacg aacgacgtcg
     1921 ccgcgcacgc acgcgacaag ctcgaccgca aacgcatcga catgatcgcg gcgaaccaag
     1981 taggtcccga ttgcggcttc gaccgcgaga cgaatgcgct cacggttttt tggcccggcg
     2041 gcggcgagct tgcgctcggc gagggcagca aaggccggct cgctcgacgg ctcgtcgagg
     2101 tcatcgccga acgctatcga gctcgacagc gagcgaagcc ttcgcccgcc gtctgatcgc
     2161 aacgcacatc agcaagggtt tcgtcgtgac cagcactgcc cgacgtgtcg agctcaagat
     2221 cctcaacccg aaaatcggca aagagttgcc gctgccgaag cgcgcaacgg cgggctcggc
     2281 aggactcgac ttacgtgcct gcctcgatgc gccgctcgat ttgagtcccg gcgataccaa
     2341 gctgattccg actgggctcg caattcacat agccgacccg cagctcgcgg ccgtcatcat
     2401 tccgcgctcg ggcctaggtc ataaacatgg cgtcgtgctc ggcaacctcg tcggcttgat
     2461 cgactcggac tatcagggcg agctcatgat ctcgtgctgg aatcgcggct cgcagacttt
     2521 tcgcgtcgag ccaggagagc ggatcgcgca actcgtcgtc gtgcccgtcg tgcaggtgga
     2581 gttcgacgtg gtcgaggatt tcgccgcgag cgaccgcggc agcggcggct tcggccacac
     2641 gggccgcagc tgatcagccg cccttgagct cgcggaagcg ggcgatgtcg ccgtcgaacg
     2701 aaaggcgcag cgcttgctga tcggagcgcg tgcgcgccag cgtctgctcg tagagcgaga
     2761 tcgacgccgt ggtccggcga atgtcgagcg ctaggttctc cgggacctgc ggcgcatcgg
     2821 gacgcgtcgt gtaaggcttg aagctgctgg cctcggcctg cagcccgacg aggcgcttgc
     2881 gcaggttgcc gagatagagc tcggtgactt tgatttgcga ctcgagcagc tcgaggcggc
     2941 ggtcgcgaag gtcttcgata tcggagacgc tcaagtaggt ctcgagcaac atgcgatcgc
     3001 ggcgcgcgat ctcggccttg gccgcacgtt ccgcggccag cacgcgctct cgctcctgga
     3061 gcaccgcgcg ctcctccgcg gtgacttcgc cttcctcgaa cgctacggcc acgccttgct
     3121 cgttcaggac attgcggtcg aggttcgcgt actcgggcgg aatattgtcg ccgtagtgga
     3181 cgacgccgtt gcggtccgtc cacttgtaca gccgcgtatc tttgtcgcgc gcctgggcgc
     3241 taccggtcga cggcagcact aaggccgcaa cgacgagcag gatggcgccg agtctcgaca
     3301 cttatgtaaa ccccaataaa cgccgcgaac gctagatata ccaaaaatcg cggcgactga
     3361 agccgccgga agttatttct gccaaatacc tctcaaatcc cagggccgct aggactcccg
     3421 ggggacgcca tagcggttgc ggtacgcgcg gacggccggc agatgctgtt cgtattcctt
     3481 atcttcctcg agatggtcga tcaggtccgt cagcgtgacg atactcctga tcggcacctg
     3541 aagctccccc tcgagctgct gcacggccga gatgcgggct ttgggggcca cttcctcgcg
     3601 gtcgagcgcc acgaggactc cggccggcgt ggctccggcg gcgcggacga tgtcgaggac
     3661 ctcgcggacc gccgttccgg ccgtgatgac gtcatcgacg atcagcacgc gcccggcgag
     3721 cggcgcgccg atgatcgtgc cgccctcgcc atggtccttc gcttccttgc ggttgaaagc
     3781 gaacggaacg ttacggccat gatgctcggc cagcgccact gcggtggtcg ccacgagcgg
     3841 gatgcctttg taagcgggcc cgaacagcac gtcgaactcg atccccgacg caagcagcga
     3901 cgcggcatag caccgcccga gcaccgcggc cgcctgcccg tcactgaaga ggccggcgtt
     3961 gaagaagtag gggctctcgc ggcc
//
LOCUS       DATLEE010000092         2784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2340527, whole genome shotgun sequence.
ACCESSION   DATLEE010000092 DATLEE010000000
VERSION     DATLEE010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340527"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VM692_01965"
     CDS             <1..1261
                     /locus_tag="VM692_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ50956.1"
                     /translation="IPVSGWVADRFGARATFVSAIAVFLLGSVGCALSDSLGALVAGR
                     FVQGAGGAMMVPVGRLVLLRTIPKSEIVEALSWVTIPALVGPMVGPPLGGFITTYFDW
                     RWIFLINLPIGALGMILGLRFIPATRQQPPPLDWFGFALAGGGLGAAMFGFSTLGQHL
                     VPIELAAAALVLGVGALIGYVVHERRREHALLDLELFRLATYRAGVVGGSLFRIGIGA
                     TPFLLPLMLQLGFGLSPVQSGLVTFVSAMGAIFMKTIAARTLKALGFRRVLLWNALLA
                     SALLCGFGLFRPSTPELVIMATLLVSGCCRSLQFTSLNAISYADVDSPRMGQASSLSG
                     MLQQLSLSLGVAIGGYSLEIFGALTGRSATELVNFYWAFVVVGLISASSAWWAWRLPR
                     NAGAEMAGRTRTARNEADPSTAELPAG"
     gene            complement(1276..1530)
                     /locus_tag="VM692_01970"
     CDS             complement(1276..1530)
                     /locus_tag="VM692_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50957.1"
                     /translation="MKTLRTFTIGCLLAAAAGVGTAAEEPSILTITVAAKHAHAPTIE
                     RVTPDATVEKTPFVMPTDMPEADIHYQLAPIGAAHAPSHE"
     gene            complement(1822..2532)
                     /locus_tag="VM692_01975"
     CDS             complement(1822..2532)
                     /locus_tag="VM692_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUQ50958.1"
                     /translation="MGSENNNKSVVVIGGTGGLGRAIAQHYADRGCDVVITGRDQERT
                     AAVAKEIGGKTRPIAVDISEPETIARPLAKLGHVDYLALVAVDRDYNSARDYSIERAR
                     KIVTLKLIGYTEVAHTLYPRMGAGASAVLFGGLASERPYPGSTSVTTVNGAVSSLIRT
                     LAVELAPVRFNAIHPGIISDTPAWADKKEAVANIEKRTPGGRLATTEDVVHAVDFMFV
                     NRGVNGINLVIDGGWLLT"
     gene            complement(2533..>2784)
                     /locus_tag="VM692_01980"
     CDS             complement(2533..>2784)
                     /locus_tag="VM692_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUQ50959.1"
                     /translation="PLEEAYFIMHGEVEAWADDKQYFMKPGDFLWAGVGCTHAFYNRS
                     NTTVRWLETQSPQPPARNSYRWSRDWEYVAQQLERKGVA"
BASE COUNT          396 a          912 c          945 g          531 t
ORIGIN      
        1 cattcccgtc agcggctggg tggccgaccg cttcggtgcg cgcgcgacgt tcgtttcggc
       61 gatagccgta ttcctgctcg gctcggtggg ctgcgcgctg agtgactcgc tgggcgcgct
      121 cgtcgccggg cgcttcgtgc agggcgccgg cggcgcgatg atggtccccg tcggccggct
      181 cgtgctgctg cgcacgatcc cgaagtccga gatcgtcgaa gcgctctcgt gggtcacgat
      241 tccggcgctc gtggggccga tggtcggccc gccgctcggc ggtttcatca cgacctactt
      301 cgactggcgc tggatatttc tgatcaatct gccgatcggc gcgctcggca tgatcctcgg
      361 gctgcggttc attccggcca caaggcagca gccgccgcca ctcgactggt tcggcttcgc
      421 gctggcgggc ggcggcctcg gcgccgcgat gttcggcttc tcgacgctgg ggcagcacct
      481 cgtgccgatc gagctcgccg cagccgcgct ggttctcggc gtcggcgcat tgatcggcta
      541 tgtcgtccac gagcggcgcc gcgagcacgc gctgctcgat ctcgagctgt tccgactcgc
      601 gacttaccgc gccggcgtcg tcggcggctc gctatttcgc atcggcatcg gcgcgacacc
      661 gttcctgctg ccgctgatgc tgcagctcgg gttcggcttg agccccgtgc aatcgggcct
      721 agtgacgttc gtttcggcca tgggtgccat cttcatgaag acgatcgccg cgcgcacgct
      781 gaaagcgctc ggcttccggc gcgtcttgct gtggaacgcg ctgctcgcat ccgcgttgct
      841 ctgcgggttc ggcttgttcc ggccgtcgac gccggagctc gtgatcatgg cgacgctgct
      901 cgtcagcggc tgctgccggt cgctgcagtt cacgagcttg aacgcgatca gctatgcgga
      961 cgtcgattcg ccgcgaatgg ggcaggccag cagcctgtcc ggcatgctgc agcaactgtc
     1021 gctgagcttg ggcgtcgcaa tcggcggcta ttcgctcgag atcttcggcg cgctcaccgg
     1081 ccgttccgcg accgagctcg tgaacttcta ctgggccttc gtcgtcgtcg gcctgatctc
     1141 cgcgagctcg gcgtggtggg cctggcggtt gccgcgcaat gccggcgccg agatggcggg
     1201 ccgcacgcgc acggcgcgca acgaagccga tccgagcacg gccgagctac cggccggctg
     1261 acccggctag gccagtcact cgtgcgacgg ggcgtgagcg gcgccgatcg gcgcgagctg
     1321 atagtgaata tcggcctcgg gcatgtcggt cggcatgacg aacggcgtct tttcgaccgt
     1381 tgcatccgga gtcacgcgtt cgatggtagg cgcatgggcg tgcttggccg ccacggtgat
     1441 cgtgaggatg ctcggctcct cggccgccgt gccaacaccc gcggccgcgg ccagcaagca
     1501 tccgatcgtg aacgttcgca gtgtcttcat ggagtgaacc tcgctctcaa tggacaccac
     1561 gttagaggat caacgtctgt agaaacatat ggaaccgccg aagaaagacg aagcggaggc
     1621 aaagattcac cgacttatct ctagagtctt gagatagcgg ctcggagctc ggctcagtcg
     1681 ttgcgcagcg cttgcatcgg cggcacgcgc agctcgcgag cgcggtgatc aggatcgagg
     1741 caagcgcgat gagcggcgac ggcgcagact gcgaagcgag ttgcgaaggt ccggcacgct
     1801 gcctgacgga agctctccgc gctacgtcag cagccagccg ccgtcgatga cgagattgat
     1861 gccgttgacg ccgcgattca cgaacatgaa gtcgacggca tggacgacgt cttcggtcgt
     1921 cgcgaggcgg ccgcccggcg tgcgcttctc gatgttggca accgcctctt tcttgtcggc
     1981 ccacgccggc gtatccgaga tgatgccggg atggatggcg ttgaaccgca cgggcgcgag
     2041 ctcgacggcc aacgtgcgaa tcaagctgct gacggcgccg ttgacggtcg tcaccgacgt
     2101 cgagcccggg tacggccgct cgctcgcgag accgccgaac agcacggccg acgcaccggc
     2161 tcccatgcgc gggtatagcg tgtgcgcgac ctcggtgtag ccgatgagct tcagcgtcac
     2221 gatcttgcgc gcacgctcga tgctgtagtc gcgcgcgctg ttgtagtcgc ggtcgacggc
     2281 gacgagcgcg agatagtcga cgtgccccag cttggcgagc ggccgcgcga tcgtctcggg
     2341 ctccgagatg tcgactgcaa tgggacgcgt cttgccgccg atctctttgg cgacggccgc
     2401 cgttcgttcc tggtcgcggc ccgtgatgac gacgtcgcag cctctatccg cgtaatgctg
     2461 cgcgatggcg cgcccgaggc cgcccgtgcc gccgatcacg acgacgctct tgttattatt
     2521 ctcgctgccc atttaggcga ctcccttccg ctccagttgc tgtgccacgt actcccagtc
     2581 gcgcgaccag cggtacgagt tgcgcgctgg cggctgcggc gattgggttt cgagccaacg
     2641 cacggtcgtg ttgctgcggt tgtagaacgc gtgggtgcac ccgactccgg cccaaagaaa
     2701 gtcgccgggc ttcatgaagt actgcttgtc gtcggcccac gcctcgacct cgccgtgcat
     2761 gatgaagtac gcctcctcga gcgg
//
LOCUS       DATLEE010000093         2984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6014910, whole genome shotgun sequence.
ACCESSION   DATLEE010000093 DATLEE010000000
VERSION     DATLEE010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6014910"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VM692_01985"
     CDS             complement(<1..851)
                     /locus_tag="VM692_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HUQ50960.1"
                     /translation="MNKLAGLGLGLAFSVTALDAGAADATLRNDIAADYREHLAALFD
                     HLHRNPELSGREMETSKRLAQELRALGYEVTEGVGKTGVVAVMRNGAGPTVLLRADMD
                     GLPVQEKSGLPNASTVHQVDILGVEQPVMHACGHDVHMTALVGTAKQLAARKSAWSGT
                     LVLIGQPAEERVSGARDMLADGLYTRFPKPDYALGFHVWSTIAAGTIMVPQTIAMSSA
                     DSVDVVVQGVGTHGASPHRGVDPVLVAAQIVVSLQSLVSRTIDPLEPGVVTVGAIHGG
                     TKHNIIG"
     gene            complement(885..1781)
                     /locus_tag="VM692_01990"
     CDS             complement(885..1781)
                     /locus_tag="VM692_01990"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ50961.1"
                     /translation="MAQIAFLGLGRMGVGMAGRLLAAGHALVVYNRTPARAEPLVRNG
                     ARLARTPREACEGADAVVAMTADDASSRATWLGDDGALAGTIAPRALAIECSTVSHAW
                     ALELGQAAAQRGLRYLDAPVTGLPPAAAAGELTLLVGAEAADLEAARPLLEALATRIL
                     HFGAVGAGTAYKLVINLIGAVQIAAAAEGLALAERAGLDTKAVVDAIATSQAASPQVV
                     RNTRRMLAGDFTHDVTFTPVLRLKDVEYALRLADDVGVGTPFGRVARDAFRRLIELGG
                     AAQNESRIIEVARGQPPPKSRL"
     gene            complement(1810..>2984)
                     /locus_tag="VM692_01995"
     CDS             complement(1810..>2984)
                     /locus_tag="VM692_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HUQ50962.1"
                     /translation="GATLTLLAPFARLASAAAKPDSRLVLVILRGGLDGLAAVPPYAE
                     PEYAKLRGPIAIGRPGSDGGVLDLDGTFGLHPALTHLHALYRSGEALVVHAAATPYRD
                     RSHFYAQNVLEAGSVDPSAANGGWLNRALAALHEAGAARDAHGAVALADSVPLVLRGD
                     VAVASWAPSQLPVPDDDTLLRVRRLYEAAAPDLAQSLYDALEIRAIAGDPSEQRMDGR
                     AGLRVAPLASAAARFLASPDGPRIAVLDAGGWDTHANQGGSSGPLAQRLRGLDLGLQT
                     LQAELGTHWRETTVLVVTEFGRAAAVNGTRGTDHGTAGCAFAVGGAVAGGRVLADWPG
                     LTARDLHDGRDLRPTTDLRALFKAVLHERFGVGEAALARSVFPASDTVEPLAGVTA"
BASE COUNT          421 a         1084 c         1070 g          409 t
ORIGIN      
        1 tcgccgatga tgttgtgctt cgtgccgcca tgaatcgcgc ctaccgtcac gacgccgggc
       61 tcgagcggat cgatggtgcg gctcacgagc gattgcagcg agacgacgat ctgtgccgcg
      121 acgaggaccg gatcgacgcc gcggtgcggc gatgcgccgt gcgtgccgac gccctgcacg
      181 acgacgtcga cgctgtcggc gctcgacatc gcgatcgtct gcggaaccat gatcgtgccc
      241 gccgcgatcg tcgaccagac gtggaacccg agcgcgtagt cgggcttcgg gaagcgcgtg
      301 tagagaccgt cggccagcat gtcgcgcgcg ccgctgacgc gctcctcggc cggctggccg
      361 atcagcacga gcgtgcccga ccaggcgctc ttgcgcgccg cgagctgttt cgcggtgccg
      421 acgagcgccg tcatgtgcac gtcgtgaccg caggcgtgca tgacgggctg ctcgacaccc
      481 aagatgtcga cttgatgcac cgtcgaggcg ttcggcaagc cgctcttctc ttggacgggc
      541 aacccgtcca tgtcggcacg cagcagcacc gtcgggcccg cgccgttgcg catgacggcg
      601 acgacgccgg tcttgccgac gccttccgtc acctcgtagc ccagcgcgcg cagctcctgc
      661 gcgagccgct tgctagtttc catctcgcgg cccgacagct cggggttgcg atgcaagtgg
      721 tcgaacagcg ccgcgaggtg ctcgcggtag tccgccgcga tgtcgttgcg cagcgtcgcg
      781 tcggcggcgc ccgcgtcgag cgccgtgaca ctgaatgcca acccgaggcc gagacctgcg
      841 agcttgttca tgaggcgctc ctcgcgacga tgcgtcctac gttactacag tcgacttttc
      901 ggcggcggct ggccgcgcgc gacttcgatg atgcggctct cgttctgcgc cgcgccgccg
      961 agctcgatga ggcgccgaaa cgcgtcgcgc gcgacgcggc cgaacggcgt gccgactccg
     1021 acgtcgtcgg ccaagcgcag cgcgtactcg acgtccttca agcgcagcac gggcgtgaac
     1081 gtgacgtcgt gcgtgaagtc gcccgcgagc atgcggcgcg tattgcgcac gacttgcggg
     1141 ctcgcggcct ggctcgtcgc gattgcatcg acgacggcct tggtatcgag gccggcgcgc
     1201 tccgcgagcg cgagaccttc cgcggcggcc gcaatctgca cggcgccgat caagttgatc
     1261 acgagcttgt aagcagtgcc ggcaccgacg gcgccgaagt gcaggatgcg cgtggccagc
     1321 gcctcgagca gcggccgcgc cgcttcgagg tcggcagcct cggcaccgac gagcagcgtg
     1381 agctcgcccg cggcggcggc cggcggcaaa cccgtgacgg gcgcgtcgag atagcgcagg
     1441 ccgcgttgtg ccgccgcctg accgagctcg agcgcccaag cgtgcgacac cgtcgagcac
     1501 tcgatcgcga gcgcgcgcgg tgcaatcgtc cccgcgagcg cgccgtcgtc gccgagccac
     1561 gtcgcgcgcg acgacgcgtc gtcggccgtc atcgcaacga cggcgtcggc accttcgcat
     1621 gcttcgcgcg gtgttcgcgc gagccgcgcg ccgttgcgca cgagcggctc cgcgcgcgca
     1681 ggagtgcggt tgtacacgac gagagcatgg ccggccgcga gcagcctgcc cgccataccg
     1741 acgcccatgc gccccaaccc caagaaagcg atctgcgcca tcggcgcagc atagcgcgcc
     1801 gcgtcttcgc tacgccgtca ctccggcgag cggctcgacc gtatcgctgg ccgggaagac
     1861 gctgcgcgca agcgcggcct cgccgacgcc gaagcgctcg tgcagcacgg ccttgaacag
     1921 cgcacggagg tccgtcgtcg gccgcaagtc gcggccgtcg tgcaaatcgc gcgccgtgag
     1981 ccccggccag tccgcgagca cgcgcccgcc cgcgacggcg ccgccgacgg cgaacgcgca
     2041 gccggccgtg ccgtgatccg tgccgcgcgt gccgttcacg gcggccgcgc ggccgaactc
     2101 cgtgacgaca agcaccgtcg tttcacgcca gtgtgtgccg agctccgcct gcaacgtctg
     2161 caatcccaag tcgagcccgc gcaatcgctg cgcgagcgga ccagagcttc cgccctggtt
     2221 cgcgtgcgtg tcccagccgc ccgcgtcgag caccgcgatg cgcggaccgt cggggctcgc
     2281 gagaaagcgc gcggccgcgc tcgcgagcgg cgcgacgcgc aagcccgcgc ggccgtccat
     2341 gcgctgctcg ctggggtcac cggcgatcgc gcgtatctcg agcgcgtcgt agaggctctg
     2401 cgcgagatcg ggcgcggcgg cctcgtagag ccggcgaacg cgcagcagcg tgtcgtcgtc
     2461 gggcacgggc agctgcgatg gcgcccacga cgcgacggcg acgtcgccgc gcagcacgag
     2521 cggcacgctg tccgccagcg cgacggcgcc gtgcgcgtcg cgcgcggcgc ctgcttcgtg
     2581 gagcgcggcg agcgcgcggt tgagccagcc gccgttcgcg gcactcgggt cgacgctgcc
     2641 cgcctcgagc acgttctgcg catagaaatg cgagcgatcg cgatacggcg tggccgcggc
     2701 gtgcacgacg agcgcttcgc cggagcggta cagcgcatgc agatgcgtga gggcggggtg
     2761 cagcccgaac gtgccgtcga gatcgagcac gccgccgtcg ctgccggggc gccctatggc
     2821 gatcgggccg cgcaacttcg cgtactcggg ttcggcgtac ggcggcacgg ccgccaaccc
     2881 gtcgaggccg ccgcgcagaa tcacgagcac gagacggctg tcgggcttcg ccgcggccga
     2941 cgcgagccgc gcgaacggcg ccagcagcgt cagtgtggcg cccg
//
LOCUS       DATLEE010000094         5159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1597998, whole genome shotgun sequence.
ACCESSION   DATLEE010000094 DATLEE010000000
VERSION     DATLEE010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597998"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..737
                     /locus_tag="VM692_02000"
     CDS             132..737
                     /locus_tag="VM692_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUQ50963.1"
                     /translation="MLLTSCAPSGPHNTGVYLLLDTSGTYREELKGAQRLIHYMLSKL
                     GPTDSFAVARIDTGSFSEQDIMAKVTLDDRPSAANVQKREFAQDVEKSLAGVKGSPYT
                     DITGGLLQAVEFLNEKQPKRKTIFIFSDLKEDLEAGYVRDIEVPVNGFEVVALNVTKL
                     RSDNVDPREYLNRLEAWRQKIEAQGGTWRVINDLDRLETLL"
     gene            complement(814..2184)
                     /gene="amt"
                     /locus_tag="VM692_02005"
     CDS             complement(814..2184)
                     /gene="amt"
                     /locus_tag="VM692_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HUQ50964.1"
                     /translation="MAVLGPALAFADTAAAPDKGDTAWMLTSTALVLLMSVPALALFY
                     GGLVRSKNMLSVLMQVFVGFSLITVLWCVYGYSFAFTTGNAFFGSTGRLFLNGLFDNS
                     AGTFALGATFSKATPMYEIVFAAFQATFAAITCCLILGSLVERIKFSAVLLFLVIWFT
                     FAYIPVAHMVWFWAGPDAYTADANADELLASAGLIWQWGALDFAGGTVVHINAGVAGL
                     VGAIVLGKRVGLGRESMPPHNLTMTMIGASLLWFGWFGFNAGSALEANGFAALAFMNT
                     FLATACAVLSWTFAEWLLKGKPSMLGAASGAVAGLVAITPAAGNVGIPGAFVIGLAAG
                     VVCLWGCTGLKKIIGADDSLDVFGVHGLGGIMGALLTGVFNAPSLGGPGLVTDWVNVT
                     MGYPGIGAQLLIQAKAVGLVVVWTAVVSFVGFYVVKAVMGLRVAEEQEREGLDITSHG
                     ERAYTG"
     gene            complement(2253..2591)
                     /locus_tag="VM692_02010"
     CDS             complement(2253..2591)
                     /locus_tag="VM692_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HUQ50965.1"
                     /translation="MKLITAVIKPFKLEDVRQALAEAGVQGMTVTEVKGFGRQKGHTE
                     LYRGAEYTVDFVPKTKIEAAVPDSLVEQVLEAITGAAGTGSIGDGKVFVFDLEHAVRI
                     RTGETGDAAL"
     gene            complement(2628..3293)
                     /locus_tag="VM692_02015"
     CDS             complement(2628..3293)
                     /locus_tag="VM692_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HUQ50966.1"
                     /translation="MRIKNLIPALALTAAAMIPAISQAEVSANIGWVSEYIYRGIHQD
                     DSSASAGIDYTSDGGFYLGTWGADVGDGLETDLYFGYAGGEDFTYKIGYTGYYYTDDF
                     DDTYTEVNLGVGYGIFALDVAIGEWDGFGTPADYTFTSITISPEKGPYYKFGSFGDDF
                     DGDYLELGYVYSIESEGIDLSIAATFSDDLPVSDSLDDGFGGESGEWALTFGIKKTIG
                     IGQ"
     gene            complement(3499..3819)
                     /locus_tag="VM692_02020"
     CDS             complement(3499..3819)
                     /locus_tag="VM692_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HUQ50967.1"
                     /translation="MVKLVVTDKDGREVEVDARPGLSIMENIRELPHSVEAVCGGLCS
                     CATCHVLIDEESMRRLPPRRYEERVMLTTSRSFDPQRSRLSCQIKVTAELEGLRLAVA
                     PDDV"
     gene            complement(3891..4232)
                     /locus_tag="VM692_02025"
     CDS             complement(3891..4232)
                     /locus_tag="VM692_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50968.1"
                     /translation="MSRAPVKDVDDLPRPATEPAGKVEFDSRGNSVWRWAKDVLGSTS
                     VLLKRLDNKDLALEPTQKIPVVGGAAEPAQKAKPQAEGRRAPVDPKAQHPAMQRERRR
                     DGGGFDPYNSR"
     gene            complement(4319..5134)
                     /locus_tag="VM692_02030"
     CDS             complement(4319..5134)
                     /locus_tag="VM692_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HUQ50969.1"
                     /translation="MAEIAIVELEDFALELARTAGGIAQAHFRQPITVENKSANAFDP
                     VTNADRAIERVLRAAIDARYPDHGIVAEEEGERAGAGEYTWFIDPIDGTRAFMTGSPL
                     WGTLIGLSRRTEPLFGLLVQPVLEEVFFGGPSGSWLIKTERRDRLKSRACKTLGKAVL
                     ASTHPDMFTGERAGAFRGLAEQCMLTRFGGDCYNYAMVAAGYLDLVVESQLKPYDIVP
                     LIPILEGAGCIVTDWHGRPPLNGGDVIAAGSREVHKAALAALRPLEVPEGERG"
BASE COUNT          953 a         1695 c         1630 g          881 t
ORIGIN      
        1 ctgcctttgt tcgtgccgct gtggctggaa gcgcgagcag gtgcgcggag cgatgcccat
       61 ccggatgccg agcccgcggc gagggaggcg tgatgttgtc ccggcggctc ttattcgcgg
      121 cggctgccgc gatgcttctg acatcgtgcg cccctagtgg gccgcacaac accggtgtgt
      181 atttgctgct cgacacctcc ggcacctatc gtgaagagct gaaaggcgcg cagcggctca
      241 ttcactatat gctctcgaag ctcggcccca cagattcgtt cgcggtggcg cgcatcgaca
      301 cgggcagctt cagcgagcag gacatcatgg cgaaggtgac gctcgacgat cgcccgagcg
      361 cggcgaacgt gcaaaagcgc gagttcgcgc aagacgtcga gaagtcgctg gccggcgtca
      421 aaggctcgcc ctataccgac atcacgggcg ggctgttgca ggccgtcgag ttcttgaacg
      481 agaagcagcc caagcgcaaa acgatcttca tcttttcaga cctcaaggag gacctcgagg
      541 ccggctacgt acgggacatc gaggtgcccg taaacggatt cgaggtcgtt gcgctcaatg
      601 tgacgaagct gcgcagcgac aacgtcgatc cgcgcgagta tctgaaccgg ctcgaagcgt
      661 ggcgccaaaa gatcgaagcg caaggcggca cgtggcgcgt catcaacgac ctcgatcgcc
      721 tcgagacgct gctctgaccg ccgtcgcaac gcgcgaaaag aaaaggccgg caacctcgcg
      781 gctgccggcc aaagctaacg gtcccagcta aagtcaaccc gtgtacgcgc gctcgccgtg
      841 cgacgtgatg tcgaggccct cgcgctcttg ctcttcggcc actcgcagac ccatgactgc
      901 cttgacgacg tagaagccga cgaacgagac gactgccgtc cacaccacga cgaggccgac
      961 ggccttcgcc tggatcagca gctgtgcgcc gattccgggg tagcccatcg tcacgttgac
     1021 ccaatcggtc acgaggccgg ggccgcccaa gcttggggcg ttgaacacgc cggtcagcag
     1081 cgcacccatg atgccgccca aaccgtggac gccgaagacg tcgagcgaat cgtccgctcc
     1141 aataatcttc ttgagccccg tgcagcccca gaggcaaacg acgccggccg cgaggccgat
     1201 cacgaacgca ccggggatgc ccacgttgcc ggcggccggc gtgatcgcaa cgaggcccgc
     1261 gacggcgccc gaggcggcgc cgagcatcga cggcttgccc ttcagcagcc actcggcgaa
     1321 cgtccacgag agcacggcgc aagcggtggc gaggaacgtg ttcatgaagg ccagcgccgc
     1381 gaagccgttt gcttcgagtg ccgagcccgc attgaacccg aaccagccga accacagcaa
     1441 cgaagcgccg atcatcgtca tcgtcaggtt gtgcggcggc atgctttcgc gccccaagcc
     1501 gacgcgcttg ccgagcacga tcgcgccaac caagcccgcg acgcccgcgt tgatgtgcac
     1561 gactgtgccg cccgcgaagt cgagcgcacc ccattgccag atcaagccgg cgctagcgag
     1621 cagctcgtca gcgttggcgt ccgccgtgta agcgtccgga cccgcccaga accagaccat
     1681 gtgcgcgacg gggatgtacg cgaacgtgaa ccagatgacg aggaacagca agacggccga
     1741 gaacttgatg cgctcgacga gcgagcccag gatcaagcag cacgtgatcg ccgcgaacgt
     1801 cgcttggaaa gcggcgaaca cgatctcgta catcggcgtg gctttgctga acgttgcgcc
     1861 gagcgcgaac gtgcccgctg aattgtcgaa caaaccgttc aagaacaggc ggcccgtgct
     1921 gccgaagaac gcgttgccgg tcgtgaacgc gaacgagtaa ccgtacacgc accacagcac
     1981 cgtgatcagc gagaagccta cgaacacctg catcaacacc gacagcatgt ttttgctgcg
     2041 gacgaggccg ccgtagaaca gcgccagcgc aggcaccgac atcagcagca cgagcgccgt
     2101 cgacgtcagc atccaggcgg tatcgccttt atcgggcgcc gccgccgtat ccgcaaacgc
     2161 gagcgccggc ccgaggactg ccatcaggcc tacgagtagg cgcagcggcg aatgagtacc
     2221 gatcttcatc gttgttttcc tcccaatctt tcttaaagcg ccgcgtcgcc ggtctcgccc
     2281 gtgcgaatgc gcacggcgtg ctcgagatcg aagacgaaca ccttgccgtc accgatcgag
     2341 cccgtacctg cggctcccgt gatggcttcc aacacttgct cgacgagcga gtccggcacg
     2401 gccgcttcga tcttcgtctt cggcacgaag tcgacggtgt actcggcgcc ccgatagagc
     2461 tcggtgtgcc ccttttgtcg cccgaagccc ttcacttcgg tgaccgtcat gccctgaacg
     2521 cccgcctcgg ccaacgcctg acgcacgtcc tcgagcttga acggcttgat gaccgccgtt
     2581 atgagtttca tctcctgatc ctcgatggtg tgttcatgtg agttgaatta ctggccgatg
     2641 ccgatcgtct tcttgatgcc gaacgtcagc gcccattcgc cggattcgcc gccgaagccg
     2701 tcgtcgagcg aatcgctgac gggcaggtcg tcgctgaagg tcgcggcgat cgagagatcg
     2761 ataccctcgc tttcgatgct gtagacgtag ccgagctcga ggtagtcgcc gtcgaagtcg
     2821 tcgccgaagc tgccgaactt gtagtacggg cccttctcgg gcgagatcgt gatcgacgtg
     2881 aacgtgtagt cggcgggcgt gccgaagccg tcccattcac caattgcaac gtcgagcgcg
     2941 aagattccgt aaccgacgcc gagattgact tcggtgtacg tgtcatcgaa atcgtcggtg
     3001 tagtagtagc ccgtgtagcc gatcttgtag gtgaagtcct cgccgccggc gtaaccgaaa
     3061 taaaggtcgg tctcgagacc gtcgccgacg tccgcgcccc aggtgccgag atagaaaccg
     3121 ccgtcggacg tgtagtcgat gcccgcggag gccgagctat cgtcctgatg aatgccgcgg
     3181 taaatgtact cgctgaccca gccgatgttc gccgagacct cggcttgcga gattgcggga
     3241 atcatcgctg ccgccgtgag ggcgagagcc gggatcagat tcttgattct catggcgctt
     3301 gttctcctga aatttgcgcg aataggattg gacggcgagc ccattgcacc atccgtgcca
     3361 cggcggcaaa gcgtgttgaa tcaaggtgtt agtcgcaaac gcaccgccgc cgtgcggcgt
     3421 gcgtgcgcca cggcggtgca ggctgcgcac caggacggag cagagagcgg ctaggaacgc
     3481 gggcggcgcg ggcgcgaatc agacatcgtc gggcgcgact gcgaggcgca gtccctcgag
     3541 ctccgcggtc accttgatct gacaactcag acgagagcgc tgaggatcga acgagcgcga
     3601 cgtcgtcagc atcacgcgtt cctcgtagcg ccgcggcggc aggcggcgca tcgactcttc
     3661 gtcgatcagc acgtgacacg tcgcgcacga gcacaagcct ccacacacgg cttcgacgct
     3721 gtgcgggagc tcgcgaatgt tttccatgat ggacagcccg ggacgcgcgt cgacttcgac
     3781 ctcacggccg tccttgtccg tgacgacgag cttgaccatg tgagtcgatt cgccggtgcc
     3841 gcaacggcgg cgactatagc agacggtcct cggcgttcgc ggcgtgcgcg ttagcgcgag
     3901 ttgtacggat cgaaaccgcc gccgtcgcgg cgccgctcgc gttgcatggc gggatgctgc
     3961 gccttcggat cgaccggggc ccgacgtcct tcggcttgcg gcttcgcttt ctgcgcgggt
     4021 tccgctgcgc cacccacgac tgggattttt tgcgtcggct cgagcgcgag atctttattg
     4081 tcgagccgct tcaacagcac ggacgtgctg ccgagcacgt ccttggccca gcgccagacg
     4141 ctgttaccgc ggctatcgaa ctcgaccttg ccggccggct ccgtggctgg ccgcggcaag
     4201 tcgtcgacgt ccttgacggg cgctcggctc atattctgtc aaacggggat agacccctgg
     4261 ggggcagcct agcgcggctc gccgccacgc cgctacgacc gagatcacac gccggctgtc
     4321 agccgcgctc cccttcgggg acttccaagg gtcgcagcgc tgccagcgct gccttgtgca
     4381 cttcacggct gccggcggcg atgacatcgc cgccattgag cggtggccgc ccgtgccagt
     4441 cagtgacgat gcacccggcg ccttcgagga tcggaatcaa cggcacgatg tcatacggct
     4501 tcagctggct ctcgacgacg agatcgaggt agccggccgc gaccatcgcg tagttgtaac
     4561 agtcgccgcc gaagcgagtc agcatgcatt gctcggccaa gccgcggaat gcgcctgcgc
     4621 gttcgccggt gaacatgtcg gggtgcgtgc tcgcgagcac ggccttgccc agcgtcttgc
     4681 acgcgcggct cttgaggcgg tcccggcgct cggtcttgat gagccagctg ccgctgggcc
     4741 cgccgaaaaa aacttcctcg agcacgggct gcacgagcag gccgaacagc ggctccgtgc
     4801 gccgcgagag cccgatcaag gtgccccaca gcggcgagcc cgtcatgaac gcgcgtgtgc
     4861 cgtcaatcgg atcgataaac cacgtgtatt cgccggcgcc cgcgcgctcg ccctcttcct
     4921 ccgcgacgat gccgtgatcg ggataacgcg cgtcgatggc cgcccgcagc acgcgctcga
     4981 tcgcacgatc ggcgttcgtc acgggatcga acgcatttgc gctcttgttc tcgaccgtga
     5041 tcggctgacg aaagtgcgcc tgagcgatgc cgcccgccgt gcgtgcgagc tccagcgcaa
     5101 aatcctcgag ctcgacgatc gcgatctccg ccatgacgct cggcccagtg cgttgtcag
//
LOCUS       DATLEE010000095         8377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1599033, whole genome shotgun sequence.
ACCESSION   DATLEE010000095 DATLEE010000000
VERSION     DATLEE010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599033"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1549
                     /locus_tag="VM692_02035"
     CDS             320..1549
                     /locus_tag="VM692_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUQ50970.1"
                     /translation="MDQASLIAQLRIERPQEEAPRRRVWWWAAAVVAVVAVGGATYAW
                     VPRAVPIRVAVAEPVTAAAAVAPSSILDASGYVVARRQATVSAKITGKVIFVGIEEGQ
                     RVERGQVIARLDDTNANASVAQARAELEQSKANLVAAEVAFANAMPTFERSEKQFARA
                     VVSAQTFDTAKASYDAARTGLNVAGRVVEVAEARLALAERNLDDTVVRAPFAGIVTVK
                     AAQEGEMVSPMSAGGGFTRTGIGTIVDMASLEVEVDVSENFINRVVAQQPATVRLNAY
                     PDWSIPARVIAIIPTADRAKATVKVRVGFDAADARILPEMGARVAFLEAAEPGAATTS
                     AAQQPVLVPKESVQADGDTGIVFVVADGGVERRTVRLGAQSASGQIVLAGLVVGTRLA
                     RGDLGALVDGSRVTIEQ"
     gene            1576..2271
                     /locus_tag="VM692_02040"
     CDS             1576..2271
                     /locus_tag="VM692_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ50971.1"
                     /translation="MTALVSIKQVVKGYTRGKQRVEVLHAIDLTVEQGDFLALMGPSG
                     SGKTTLLNLIGGLDRPDRGEITVAGQRIDELSSGQLAQWRARNVGFIFQFYNLLPVLT
                     AERNVEVPLLLTNLSSAQRKRNVAAALELVGLSDRAKHKPSELSGGQQQRIAIARALV
                     ADPTLLVCDEPTGDLDRETSESILKLLNLLNEQQGKTIVMVTHDPRAAQYASRRLYVD
                     KGSLTTAALGVAA"
     gene            2268..3419
                     /locus_tag="VM692_02045"
     CDS             2268..3419
                     /locus_tag="VM692_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ50972.1"
                     /translation="MKYLPLVWAGLWRKRARTVLTLCCIVVAFGLFGILHGFTAAIDG
                     IIDAMSDTRLRSMSRVNIMEALPLAHAQRMATVPGVQAVSYYNFFAGYYQETRNSVQV
                     GAIDVESFNVVYPDIQLEPQYIEAMKQTRNGALVGEDLAKERGWKVGDRIPLGSAVWR
                     RKDGAESWDFEIVGSYRSPSGRVPTNELWINYAYFDEARADGNGTVTIYFLKIADPNR
                     AAEISEEVDKLFANSMFETQTQSEKDWLRAQIGQIGDIGFFVNAIIAAVMFALLFVTG
                     NTMMQSVRERIPELVVLKTYGFSNLGIVSLVIAESLLLCSVAAALGIGIAAAISPSLY
                     REFGAGGLNLPLSVIGMGLGLAAVFAVVSALLPALRAQRLSIVDALAGR"
     gene            3446..4627
                     /locus_tag="VM692_02050"
     CDS             3446..4627
                     /locus_tag="VM692_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ50973.1"
                     /translation="MSVLKEIASVTGMNLRSVPQRLASSSVVVVGIAGVVAVMMSVSA
                     LTRGLSGAVLATGSADRAIVLRAGATGELSSTLLMDAVATIKDAPGIARADGGGAAAS
                     AEFETGVNLLRKENGARAGLSVRGVEPSFMAVRPEISIIEGRSFNPGLREVLVGRGAH
                     AEFRGVAIGDEVELRNSRWTVVGVFESDGDAHESSLMADAATLMSAYQRTAVNSVTVR
                     LESAAAFDGFKAALTTNPTLSVSVERESDYYLRESGEAGDVFRIVGAAVAIFMGLGAI
                     FAALNTMYSAVSARAREIATLRAIGFGAGGVVVSVLVEALVLSLIGASVGAAISWLLF
                     NGNTVTLGSSTDSLIFEMQVTPALLGLGIALAVGVGLIGGLFPAVRAARLPVATALRA
                     V"
     gene            complement(4633..5229)
                     /locus_tag="VM692_02055"
     CDS             complement(4633..5229)
                     /locus_tag="VM692_02055"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUQ50974.1"
                     /translation="MKLRKTLLLVAIGVCYVSSSLPATLRTVDVSRDDDRYKVVADTH
                     LDASPEAIYAVLLDFDANHYQRISEIYKESSYLPPDRDGTPVVYTRVEGCLLWYCRSM
                     RRVERLEVVTPQFIRSRTVPERSDFKYTLSEWTFAAEGTGTRLTYMMEMEPSFWLPPF
                     VGPWFLKRTLLQGAPKAIDQIELLAQQEERAATGAAAR"
     gene            5288..6040
                     /locus_tag="VM692_02060"
     CDS             5288..6040
                     /locus_tag="VM692_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HUQ50975.1"
                     /translation="MAATTAALAQQDDEPGRVRAVVSDVKGYVTAPLHAERKQWVRFG
                     AALGAVAVAYQLDDDVRDHFGTVTVPVGTSADTHDARDAAPAALAVGGTWLAAVLSDQ
                     DDGKREAGAMLESAAFSGVAAYALKQLAGRERPYVTADESDWGGDGEAFPSVHTAAAF
                     AIGTVLAESGNERHRWLRRTLGYGLAVGTAYARMDHGAHWLSDTVAGAGLGIATARFV
                     MKQSDERARRARFGLVPSADGVAIGYTVDLRP"
     gene            complement(6048..6404)
                     /locus_tag="VM692_02065"
     CDS             complement(6048..6404)
                     /locus_tag="VM692_02065"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HUQ50976.1"
                     /translation="MKNQPLTRRLLFALAGLSSAWRTENSFKTHVVAAVAVVAVLLWR
                     GATPLWWAIAALTIAFVLAAELFNTAVEGLADHLHPDQHPAIRTVKDCAAGAVLIASA
                     AALGVAAAFVYDVALR"
     gene            complement(6401..7321)
                     /locus_tag="VM692_02070"
     CDS             complement(6401..7321)
                     /locus_tag="VM692_02070"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HUQ50977.1"
                     /translation="MNDGGVAERAAYAGFWRRFAAYSIDYFVVLVAGIVLGALVLRAG
                     IVADGTQGRFTLWLALGYFLYCALLESSSWQATVGKRAMGIRVTNRRGERIAFARAAV
                     RFVAKLLSVLTLFLGYLLAALTPRRQALHDLIAGTLVTHDSVRHTPTWVVAAISVVAG
                     VPLLAAAAAIAVPAYQDYAIRAQVTEGLDLANGYRRAVEAAWRNSSRDFAELTSDSVA
                     AGLPRAGRYVESIEVVSGMIVISYGGAANESLAGSVLALVPALDRERTLGWACGYGPA
                     PTGFATVFEGHAGYTDIDERYIPSLCRSTP"
     gene            complement(7331..8092)
                     /locus_tag="VM692_02075"
     CDS             complement(7331..8092)
                     /locus_tag="VM692_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUQ50978.1"
                     /translation="MDLRGKTVLITGAGRGLGAAMAKRLAARGADLALVDLEEAGMAA
                     VRDACRQLKVRAETYAANVAEESDVVATFDRVVADFGRLDGSIANAGILRDGLLLKAK
                     DGEVTGKLSVEQWQAVIDVNLTGVFLCGREAAERMIRLKSGGCIINISSLSRAGNFGQ
                     SNYSAAKAGVAALTVVWAKELARHEIRVNAIAPGFIKTEMVASMKPEILEKMAAQIPA
                     QRLGEPDEVAATAEFIFANDYLNGRILEIDGGQRL"
     gene            8091..>8377
                     /locus_tag="VM692_02080"
     CDS             8091..>8377
                     /locus_tag="VM692_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HUQ50979.1"
                     /translation="MPHLEKPPLQLSNAIVAPASRRHQKQANYGMLAGPNESNIRPAR
                     PRMLLSKYFDRVAIVLSTICIVHCLAMPFVIAVLPVAAFAVGGDGHFHSLML"
BASE COUNT         1395 a         2671 c         2830 g         1481 t
ORIGIN      
        1 gtgcctttgt cagtcgtcgc gaagcatcgg cctcaacttg cgccgttgtc aaagccgccg
       61 tgtaagtcgc gccgcggcta tggtgcaccg ctgcgctcgc tgcacttatg tcacgcccgc
      121 agcgagcgcg gcggcccgca actgttgtcg cggccagcca cgacttagcg gaattcgccg
      181 cgcggcatgc gcacgctgcg cctgccgctg taaccattcc tgcgccgaaa catctctcgg
      241 ttcaggtgct tacatcgcgc gcactgccgc tggcatggat ttcgctaaag gcttgcgtcg
      301 ggtgaaggag ggagtcgccg tggatcaagc ctcgttgatc gcgcagttgc gcatcgagcg
      361 cccccaagag gaagcgccgc gccgcagggt gtggtggtgg gcagcagcgg tcgttgcagt
      421 cgtcgcggtc ggcggggcca cgtacgcttg ggttcctcgc gcggtaccga tccgcgtcgc
      481 ggttgcggag ccagtcacgg cagcggccgc cgttgcgccg agctcgatcc tcgacgcttc
      541 cggctacgtc gtcgcgcgca ggcaggccac ggtctccgcg aaaatcacgg gcaaagtgat
      601 tttcgtcggc atcgaggaag ggcagcgcgt cgagcgcggc caagtcatcg cgcgcttgga
      661 cgataccaac gcaaacgcaa gcgtcgctca ggcgcgcgcc gagctcgagc agagtaaggc
      721 gaatctcgtc gcggcggaag tcgcgttcgc caacgcgatg ccgacgttcg agcgcagcga
      781 gaaacagttc gcgcgcgccg tggtcagcgc gcagacgttc gatacggcga aagccagcta
      841 cgatgcggcg cgcacggggc taaacgtcgc ggggcgtgtc gtagaggtcg ccgaagccag
      901 gctggctctc gccgagcgca atctcgacga cactgtcgtg cgagcgccgt tcgccggcat
      961 cgtcaccgtc aaggctgcgc aggaaggcga gatggtttcg ccgatgtcgg ccggcggcgg
     1021 tttcacgcgc accggtatcg gcacgatcgt ggacatggcg tcgctcgagg tcgaggtcga
     1081 cgtcagcgag aacttcatca accgcgtcgt tgcgcagcag ccggccacgg tgcggttgaa
     1141 cgcgtatccc gactggagca tcccggcgcg cgtcatcgcg atcattccga cggccgatcg
     1201 cgccaaggcc accgtcaaag tgcgcgtcgg cttcgacgcc gcagatgccc gaatcttgcc
     1261 cgagatgggc gcgcgcgtgg cgtttctcga ggccgctgag ccaggcgccg cgactacgag
     1321 cgccgctcag cagcccgtgc tcgtgccgaa ggagtcggtg caggccgacg gcgataccgg
     1381 aatcgtcttc gtcgttgccg acggcggcgt cgagcggcgc accgtgcgac tcggcgcgca
     1441 aagcgcctca gggcagatcg ttcttgcagg cctcgtggtc ggcacgcgct tggcgcgcgg
     1501 cgatctcggt gcgctcgtcg atggttctcg ggtcactatc gagcagtaaa gctcgaacga
     1561 caaaggagtc gctcaatgac ggcgttggtt tccatcaagc aagtggtcaa gggctatacg
     1621 cgcggcaagc agcgcgtcga ggtgctgcac gcgatcgact tgacggtcga gcagggcgac
     1681 tttctggcgc tcatggggcc ctcgggctct ggtaagacga cgctgttgaa tctgatcggc
     1741 gggctcgacc gacccgatcg cggtgagatc acggtcgcgg gccagcgcat cgacgagctg
     1801 tcgtcgggcc agctcgcgca atggcgagcg cgcaacgtcg gcttcatctt ccagttctac
     1861 aacctgctgc ctgtgctgac cgcggagcgc aacgtcgagg ttccgctgtt gctgacgaac
     1921 ctttcgagcg cgcagcgcaa gcgcaatgtc gccgctgcgc tcgagctcgt cgggctctcg
     1981 gatcgcgcca agcacaagcc gtccgagcta tcgggcggtc agcagcagcg catcgcgatc
     2041 gcgcgcgcgc tcgtcgccga tccgaccctg ctcgtctgcg acgagccgac gggcgacctc
     2101 gatcgcgaga cttcggaatc gatcttgaag ctcttgaatc tgctaaacga gcagcagggg
     2161 aagaccatcg taatggtcac gcacgatccg cgagccgcgc agtacgcgtc gcgccggttg
     2221 tacgtcgaca aaggctcgct gacgacggcg gcgctcggag tcgcagcatg aagtatctgc
     2281 cgctcgtgtg ggccggtctg tggcgcaagc gcgccagaac cgtgctgacg ttgtgctgca
     2341 tcgtcgtcgc cttcggcctg ttcggcatcc tgcacggctt cacggcggcc atcgacggca
     2401 tcatcgatgc catgagcgat acgcggctgc gcagtatgag ccgagtcaac atcatggagg
     2461 cgttgccgtt ggcgcacgcg cagcgcatgg cgaccgtgcc cggagtacag gccgtctcgt
     2521 actacaattt ttttgccggc tactaccaag aaacgcgcaa ttcggtccag gtcggcgcta
     2581 tcgacgtcga gagcttcaac gtcgtctacc ccgacatcca gctcgagccg cagtacatcg
     2641 aagccatgaa gcaaacgcgt aatggcgcgc tcgtcggcga agatctcgcc aaagagcgcg
     2701 gctggaaagt cggcgatcgc attccgctgg gttctgcggt ctggcggcgt aaagacggcg
     2761 cggagagctg ggatttcgag atcgtcgggt cttatcgctc gccgtcgggg agggtgccga
     2821 cgaacgagct ctggatcaac tacgcgtatt tcgacgaggc tcgcgccgac ggcaacggga
     2881 cggtgacgat ttatttcttg aagattgcgg atcccaaccg tgccgccgag atctcggagg
     2941 aagtcgacaa gttgttcgcg aattcgatgt tcgaaacgca gactcagagc gagaaagact
     3001 ggctccgcgc ccagatcggg cagatcggcg acatcggttt cttcgtcaac gccatcatcg
     3061 cagccgtgat gttcgcgctg ttgttcgtga cgggtaacac gatgatgcaa tccgtgcgcg
     3121 agcggattcc cgagctcgtg gtcttgaaga cttacggttt cagtaaccta ggcatcgtta
     3181 gcctcgtcat tgccgagtct ttgctgctct gcagcgtggc ggccgcgttg ggcatcggca
     3241 tcgcggccgc gatttcgccg tcgctctatc gcgaattcgg cgctggcgga ttgaacttgc
     3301 cgttgtccgt catcgggatg gggctcggct tggccgcggt gttcgccgtc gtcagcgcgc
     3361 tgctgcccgc gttgcgagcg cagcgtctca gcattgtcga cgccctcgca ggccgttgac
     3421 tctcgcacga aggagatctc ggtacatgag cgtgctcaaa gaaatcgcgt cggttaccgg
     3481 aatgaatctg cgcagcgtgc cacagcgcct cgcgagctcg tcggtggtcg tagtcggcat
     3541 cgccggcgtc gtggccgtca tgatgtcggt gtctgcgctg acccgcggcc tatcgggcgc
     3601 cgtgctcgcg acgggcagcg ccgaccgtgc catcgtgcta cgcgccggcg caaccggcga
     3661 gctttcgagc accctgctca tggacgcggt cgcaacgatc aaggatgcgc ccgggattgc
     3721 tcgagcagac ggcggcggcg cggcggcgtc ggcggagttc gagactggag tcaacttgct
     3781 gcgcaaagag aatggtgcac gcgcgggcct atcggtgcgt ggcgtagagc cctcgttcat
     3841 ggcggttcgg ccggagatca gcatcatcga aggccgcagc ttcaaccccg gtttgcgcga
     3901 ggtgctcgtg gggcgtggcg cgcatgcgga gtttcgcggc gtagcaatcg gcgacgaggt
     3961 cgagctgcgc aacagccggt ggaccgtcgt cggcgtcttc gagagcgacg gtgatgcgca
     4021 cgagtcgagt ctcatggccg acgctgcgac gctgatgtcg gcgtatcagc gcaccgcggt
     4081 caacagtgtg accgtgaggc tcgagtccgc cgctgcattc gacgggttca aggccgcgtt
     4141 gactacgaat ccgaccttgt ccgtgtctgt cgagcgcgag tccgattatt acctgcgaga
     4201 atccggcgag gccggcgacg tcttccgcat cgtcggggcc gctgtggcaa ttttcatggg
     4261 tctcggcgcg atctttgccg cgctcaacac gatgtattcg gccgttagcg cccgagcgcg
     4321 cgagatcgca acgttgcgtg cgatcggctt cggcgccggc ggcgtcgtag tctccgtcct
     4381 cgtcgaggcg ctcgtgctgt cgctgatcgg cgcctccgtc ggcgcagcga tttcgtggct
     4441 gctgttcaac ggcaacactg tgacgctcgg cagcagcacc gattcgctca tcttcgagat
     4501 gcaggtgacg ccggcgctgc tcggcctcgg catcgcgttg gccgttggcg tcggcctcat
     4561 cggcggcttg tttccagcgg tgcgcgcagc gcggctgccg gttgcaacgg cattgagggc
     4621 ggtctaggcc gtttagcgcg cggcggcgcc ggtcgccgcg cgctcttctt gctgcgccag
     4681 cagctcgatc tgatcgatcg ccttcggcgc gccttgcagc agcgtccgct tcaggaacca
     4741 ggggccgacg aacggcggca gccagaagct cggctccatt tccatcatgt aggtcaggcg
     4801 cgtgccggtg ccttcagccg cgaacgtcca ctcggacagc gtgtacttga agtcgctgcg
     4861 ttcaggtacg gtgcgggatc gaataaactg cggcgtcacg acctcgaggc gctcgacgcg
     4921 gcgcatgctt ctgcaatacc acaacaggca gccctcgacg cgcgtataaa cgacgggagt
     4981 gccgtctcgg tcgggcggta ggtagctgct ctccttgtat atctcggaaa tccgttgata
     5041 gtgattggcg tcgaaatcga gcagcaccgc atagattgcc tccggcgacg cgtcgaggtg
     5101 cgtatcggcg acgaccttat agcggtcgtc atcgcgcgat acgtcgacgg tccggagcgt
     5161 cgcgggtagg ctcgaggaga cgtagcacac gccgatggca acgaggagca gcgttttgcg
     5221 caatttcatc agcgggcgtc gcaacttcgg cgtttggagc aggcgaaaac tatacgctgc
     5281 cgtcgccttg gccgcgacga cagcggcgct cgctcagcaa gacgacgagc cgggccgcgt
     5341 tcgcgcggtc gtctccgatg tgaagggcta cgttacggcg ccgctgcatg cggagcgcaa
     5401 gcaatgggtg cgattcggcg ccgcgctcgg tgcagtcgca gttgcgtatc agctcgacga
     5461 tgacgtgcgc gatcacttcg gcacggtcac cgttcccgtc ggcacgagcg ccgatacgca
     5521 cgatgcacgc gatgcggctc cggcggcgct cgcggtcggc gggacctggc tcgcggccgt
     5581 gctctcggac caggacgacg gcaaacgcga ggcgggcgcg atgctcgagt ccgcagcctt
     5641 cagcggcgtc gcggcctatg cgctgaagca attggccggg cgcgaacggc cgtatgtcac
     5701 ggccgacgaa agcgattggg gcggcgacgg cgaagcgttt ccgtccgtgc acaccgcggc
     5761 cgcgttcgcg atcggcaccg tgctcgcgga gtccggcaac gagcggcacc gttggttgcg
     5821 tcgcacgctc gggtacggcc tcgcggtcgg caccgcctac gcgcgcatgg atcacggcgc
     5881 gcactggttg tcggacaccg tcgcaggcgc gggcctcgga attgcgacgg cgcggttcgt
     5941 catgaaacag agcgacgagc gggcgcggcg cgcgcgcttt gggctcgtgc cgagcgccga
     6001 cggcgtcgca atcggttata ccgtcgacct acggccctga agccgtatca gcgcagcgcg
     6061 acgtcgtaga cgaacgcggc ggcgacgccg agcgccgcgg cgctcgcgat tagcactgcg
     6121 ccggccgcgc aatccttgac cgtcctgatc gccggatgct gatccgggtg cagatgatcg
     6181 gccaggcctt cgacggccgt gttgaagagc tcggctgcca gcacgaacgc aatcgtcagg
     6241 gccgcaatgg cccaccacag cggcgtcgct ccgcgccaga gcagcacggc aacgacggca
     6301 acggccgcga cgacgtgggt cttgaagctg ttctcggtcc gccaagccga gctcaagcct
     6361 gcgagggcga acaatagtcg gcgcgtcaac ggttggttct tcatggcgtg ctgcggcaca
     6421 aggatggaat gtagcgctcg tcgatatccg tgtagccggc gtggccttcg aacaccgtcg
     6481 cgaatccggt gggcgccggc ccgtagccgc aggcccagcc gagcgtgcgc tcacgatcga
     6541 gcgccggcac gagcgccaag acgctgcccg caagagactc gttggccgcg ccgccgtaac
     6601 tgatgacgat catgcccgag acaacctcga tcgattcgac gtagcggccc gcgcgcggaa
     6661 ggcccgctgc gacggaatcc gaagtcagct cggcgaagtc acgcgacgag ttgcgccaag
     6721 cggcttcgac ggctctgcga tagccgttcg cgaggtcgag gccctccgtt acttgggcgc
     6781 gaatcgcgta gtcttgatac gccggcacgg caatagccgc ggccgccgcg agcaatggca
     6841 cgccggccac gacgctgatc gcggcgacga cccacgtcgg cgtgtggcgc acgctgtcgt
     6901 gcgtgacgag agtgccggcg atcaggtcat gcaatgcttg gcgccgcggc gtgagcgcgg
     6961 ccagcagata acccaggaac aacgtgagca ccgataggag cttcgcgacg aagcgcacgg
     7021 ccgcgcgggc gaaagcgatg cgttcgcctc tgcggttcgt gactctgatg cccatcgcgc
     7081 gtttaccgac cgtcgcttgc cacgacgagc tctcgagcag cgcgcaatac aagaagtagc
     7141 cgagcgcgag ccacagcgtg aagcgccctt gcgtgccgtc cgccacgatg ccggctcgga
     7201 gcacgagggc gccgagcacg atgccggcga ccagcacgac gaagtagtcg atcgaataag
     7261 ccgcgaaccg ccgccagaag ccggcatagg ccgcgcgttc cgccacgccc ccgtcgttca
     7321 cctgaatctc ctagagccgc tgtccgccgt cgatctcgag gatgcgtccg ttcagatagt
     7381 cgttggcgaa gatgaactcg gccgtggcgg cgacctcgtc gggctcgccg agccgttgtg
     7441 cgggaatctg cgcggccatc ttctcgagta tctcgggctt catgctggcg accatttcag
     7501 tcttgatgaa gcccggagcg attgcattga cgcggatctc atgccgcgcg agctccttgg
     7561 cccagacgac ggtcagcgcg gcgacgccgg ctttggcggc cgagtaattg ctttggccaa
     7621 aattgccggc gcgcgacagg ctcgagatat tgatgatgca gccgccgctc ttgagacgga
     7681 tcattcgctc ggctgcctcg cggccgcaga ggaacacgcc cgtcaggttg acgtcgatga
     7741 cggcttgcca ctgctcgacg ctgagcttgc cggtgacctc gccgtccttc gctttgagga
     7801 gcaaaccatc gcggaggatt ccggcgttgg cgatgctgcc gtcgagccgc ccgaaatcgg
     7861 ccacgactcg atcgaacgtc gccacgacgt cgctttcctc cgcgacgttc gcagcgtaag
     7921 tctcggcgcg cactttgagc tgccggcacg cgtcgcggac ggcggccatg ccggcctcct
     7981 cgaggtctac gagcgccaag tcggcgccgc gcgcggcgag gcgcttcgcc atggcggcgc
     8041 caagcccgcg gccggcgccg gtgatcaaga cagtcttacc gcgaaggtcc atgccgcacc
     8101 tcgagaaacc gccgctgcaa ttgagtaacg cgatagtagc gccagccagt cggcgccacc
     8161 agaaacaggc aaactacggt atgctcgccg gcccgaacga atcgaacatt cgacctgctc
     8221 gaccccgcat gttgctctcg aagtacttcg accgcgtcgc cattgtgctt tcgacgattt
     8281 gtatcgtgca ttgccttgcg atgccgttcg tcatcgcggt cctgcccgtg gcggccttcg
     8341 cggtcggagg cgacggtcac ttccattcgt tgatgct
//
LOCUS       DATLEE010000096         3043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4576862, whole genome shotgun sequence.
ACCESSION   DATLEE010000096 DATLEE010000000
VERSION     DATLEE010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4576862"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="VM692_02085"
     CDS             complement(<1..1030)
                     /locus_tag="VM692_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50980.1"
                     /translation="MKRVIPESLIVLCAALCACSGDGGSSAASSPSASPNPPPAAAAT
                     ERIVYSSLRPGNWDIYYFAGAGAAPRRLTDHPGLDYDAALSPDGRWVVFTSERRGNPD
                     LYALEVERGGEPRLLIDSAAMEDQVTFSADGRSIAFVSTASGNADIYVLPFQPDATQS
                     LTAATNVTNDPGGDFRPDFSPDGARIAFATDRDTPVEGHPIFGFTRQREGEIYVMNRD
                     GSGLQRLTRSPDWDGSPEWSPDGRTLYFYSARPREIVGPPTSPILGQEGGFRIWAMDA
                     DGSNARAVTPEGVEALAPAVTADGRIAYQKRTGYARWSIESIAGDGSGTRAESDSATD
                     YWLPDFHSS"
     gene            complement(1112..2368)
                     /locus_tag="VM692_02090"
     CDS             complement(1112..2368)
                     /locus_tag="VM692_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50981.1"
                     /translation="MGFAIVGVSLFLLAVTPLSYAFNDHGPLSMLMFWLGLGGESNLG
                     AWWSGVLFLLSAIFALDRSSETERTPVERRGWAALAAALVLLSFDEIAALHEFLSARN
                     RAYLIPLGLLGLGLVGYSLIHLRRAGVSLRRLLLAFALLASVPIQERVQQTHEWRDVV
                     IYGVRVLLEEGTEIVAALLLVAGTSGGLLRLRMGPRTFASLARFGTPLLWLALIALPI
                     AALAVYPVNLAGASNWLGTTLFLCCALLAQRQAALRRDPVLLMAAALYLLASLGANAV
                     RPDWDPLVLGVEVKLRGVYFGTLLLAAPWVLSAGQPWRQRAFWLALAGCTLLAAFLLV
                     RPQVVWSTWPPTIALLCFYIEISAAMRPRTAIARDAAVATNPGVTRRETTRGTPDSET
                     TLGHPGAEAMQASNAAPKAPTIATPF"
     gene            2627..>3043
                     /locus_tag="VM692_02095"
     CDS             2627..>3043
                     /locus_tag="VM692_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50982.1"
                     /translation="MRKQIIAALLCVPCAANAEKLLVEYQGTVSSVAQGTDAAPLPYA
                     VGDSIGGRLTIDASLAPRDTLLADATIGRYSGGIDFILGPSRPIEVGQSNSDLLLVYD
                     DWTSATTGAGPQDGFLIHDRSIGIDGEFNLVLGMLRP"
BASE COUNT          586 a          987 c         1030 g          440 t
ORIGIN      
        1 cgctcgagtg gaaatccggc agccagtagt cggtcgccga atcgctctcg gcgcgcgtgc
       61 ccgagccgtc gcccgcgatc gactcgatgc tccagcgcgc gtagcccgtg cgcttctggt
      121 acgcgatgcg gccatccgcc gtgacggcgg gcgccagcgc ttcgacgcct tccggcgtca
      181 cggcgcgcgc gttcgagccg tcggcgtcca tcgcccaaat ccggaagccg ccttcttggc
      241 ccaggatcgg gctcgtcggc gggccgacga tctcgcgcgg ccgcgcggag tagaaataca
      301 acgtgcggcc gtccggcgac cactcgggcg aaccgtccca atcgggcgac cgcgtcagcc
      361 gctgcaaacc gctgccgtcg cgattcatga cgtagatctc gccttcccgc tggcgcgtga
      421 aaccgaaaat cgggtggccc tcgacgggcg tgtcgcggtc cgtcgcgaac gcgatgcgcg
      481 cgccgtccgg cgagaagtcg ggccggaaat cgccaccggg atcgttggtc acgttcgtcg
      541 cggctgtaag gctttgcgtt gcatcgggct ggaacggcaa cacgtagatg tcggcattgc
      601 cgctcgccgt gctcacgaac gcgatgctgc ggccgtcggc ggagaacgtg acctgatcct
      661 ccatcgcggc actgtcgatc agcaggcgcg gctctccgcc gcgttcgacc tcgagcgcat
      721 agagatcggg attgccgcgg cgctcggacg tgaatacgac ccaacggccg tcgggcgaga
      781 gggcggcatc gtagtcgagc cccggatgat cggtgagccg ccgcggcgcg gcgcccgcgc
      841 cggcgaagta gtagatgtcc cagttgccgg ggcgcagtga cgaataaacg atgcgctcgg
      901 tggcggcggc cgccgggggc gggttggggc tcgcgctcgg ggacgaggcg gcagacgacc
      961 ccccgtcacc gctacaagcg cagagcgccg cacataatac gatcagcgat tccggtatga
     1021 cgcgcttcat gtcggattcc ccctctgcga catcgccagc tgcagcgatt gtcgaggcaa
     1081 gaggcggaga aaacaaacag cggacaaggg ttcagaacgg ggttgcgatg gtcggcgctt
     1141 tcggcgcggc gttggaggcc tgcatcgcct ccgcgccggg atgcccgagc gtcgtctcgc
     1201 tatctggggt gccacgggtc gtttcccgtc gggtaactcc cgggttcgtc gcgacggcag
     1261 cgtcgcgcgc aatggccgtg cgcggacgca tcgcagcgga gatttcgatg tagaaacaga
     1321 gcaacgcgat cgtcggcggc catgtcgacc agacgacctg cggccgcacg agcaggaacg
     1381 ccgccagcaa cgtgcaaccc gcgagcgcga gccagaaggc tcgctggcgc caaggttgcc
     1441 cagccgaaag cacccacggc gccgcgagca gcagcgtccc gaagtagacg ccgcgtagct
     1501 tcacctcgac accgagcacg agcggatccc agtcgggacg cacggcattc gcgccgagtg
     1561 aagccagcaa atacagtgcg gccgccatga gcaaaaccgg gtcgcggcgc aacgccgctt
     1621 ggcgctgcgc gagcaaggcg cagcagagga ataacgtcgt accgagccag ttagaagcgc
     1681 cggcgaggtt gacggggtag acggcaagag cggcaatcgg cagcgcgatg agcgcgagcc
     1741 acaaaagcgg cgtgccgaac cgagccaacg atgcgaacgt tcgcggcccc atgcgcagcc
     1801 gcagcaggcc tccactggtc ccggcgacca gcagcagcgc ggcgacgatc tccgtgcctt
     1861 cttcaagcaa aacccggacg ccgtagatga cgacgtcccg ccactcatgc gtttgctgca
     1921 cgcgctcttg aatcgggacg ctggcgagga gggcgaaggc cagcagcaac cggcgcagcg
     1981 agacgcccgc acgacgcaag tgaatcagcg agtaacccac gaggccgagg ccgaggaggc
     2041 ctaagggaat caggtatgcg cggttacgcg cgctcaagaa ctcatggagg gccgcgatct
     2101 cgtcgaacga tagcagcacg agagccgcag cgagcgcggc ccacccgcgc cgttcgaccg
     2161 gcgtgcgctc ggtctccgag ctgcggtcga gggcgaagat ggcactaagc aggaacagca
     2221 cgcccgacca ccaggcgccg aggttgcttt cgccgccgag cccgagccag aacatcagca
     2281 tcgataacgg gccgtggtcg ttgaacgcgt acgacaacgg cgtcaccgcc aacaagaaca
     2341 gcgagacgcc gacgattgcg aaccccatta gagagatccg gtgaggcaca acgagcagac
     2401 cttcccctat tggtgccgac gaactgtggc tgcgccgaaa cagtctagta acgagcaaga
     2461 gccatgccat tgaatggctt gtgaccagcc gcgtaaagcc gttgcaagaa gtgcgaaaac
     2521 gcgagggagc gcgtcgtgtt gcctatagac aaacggccga gtcgacaccg gggcatgtgt
     2581 cttgcattga aaccggtgca gacgataaag aattttggga ggaactttga gaaaacagat
     2641 tatcgccgcg ttgctctgcg tcccgtgtgc agccaacgcc gagaagctac tcgtcgagta
     2701 ccaggggacc gtgtcttcgg tcgcccaggg taccgatgcc gcgcccttgc cctacgccgt
     2761 cggcgattcg atcggcggaa ggttgacgat cgacgcgagc ttggcgccgc gcgacacgct
     2821 gcttgccgac gcgacgatcg gccgctactc gggaggcatc gacttcatct tgggcccgag
     2881 ccgcccaatc gaggtcggcc agagcaactc cgatctgttg ctcgtttacg acgactggac
     2941 gtcggcgacc accggggccg ggccgcagga cgggttcttg atccacgacc gctcgatcgg
     3001 catcgacggc gagttcaacc tcgtgctcgg catgctgcgg ccg
//
LOCUS       DATLEE010000097         2985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2343789, whole genome shotgun sequence.
ACCESSION   DATLEE010000097 DATLEE010000000
VERSION     DATLEE010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2343789"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /gene="argS"
                     /locus_tag="VM692_02100"
     CDS             complement(<1..852)
                     /gene="argS"
                     /locus_tag="VM692_02100"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HUQ50983.1"
                     /translation="MPSSPNESSQAQFPRAALAARLQRAVAKAFGPEHANVDAMIRRS
                     ERADFQADLAMGLAKPLKRPPRQVAEAVVAAAELADICERVEIAGPGFINLTLQASFL
                     ESALAAVARDARLGVPLAAHQERVVIDYSAPNVAKEMHVGHLRSTIIGDALARLLEFA
                     GHDVVRQNHIGDWGTPFGMLIEHLLDVGGSGEQASVGELSDFYRAARVKFDDDPAFAD
                     RARQRVVLLQAGDARTLELWRQLVDLSYRYFASVYERLGIGLLPAHVRGESAYNDALA
                     DVAAELER"
     gene            937..1311
                     /locus_tag="VM692_02105"
     CDS             937..1311
                     /locus_tag="VM692_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ50984.1"
                     /translation="MLPSPVQDLKAFVPARDYTLAKQFYLDLGFTLDWSSDQVAEFSA
                     GSFRFLLQPFYVKEHAENFMMSLNVDDTDRWWEHIERIELVAKYPGIMARAPAMQPWG
                     LRVLYVSDPTGVLWHITERPKA"
     gene            complement(1316..2287)
                     /locus_tag="VM692_02110"
     CDS             complement(1316..2287)
                     /locus_tag="VM692_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HUQ50985.1"
                     /translation="MKAIRVHACGGPDALRYDEVSVPVPQAGEALVEIAAAGVNFIDV
                     QHRTGRYKPPALPFTLGSEAAGVVTAVGPGVTEVAVGDRVAYAMVLGSYAEYAVVPAR
                     RLVKVPAAVELETAAAVMLQGMTAHYLTHSTFEIKPGDVALVHAAAGGAGQLITQVAR
                     LRGATVYGTVGGAAKAAVARAAGAAVTIDYRSQDFEAEVKRLTNGRGVDVVYDSVGKD
                     TFDKSLNSLRPRGMLALFGFSSGAVPPFDPAVLGAKGSLFLTRPGLNQYIATRDELVG
                     RANDLFEWLASGKLTITIDRVMPLAEAAQGHEALEARQTAGKIVLVP"
     gene            2378..>2985
                     /locus_tag="VM692_02115"
     CDS             2378..>2985
                     /locus_tag="VM692_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HUQ50986.1"
                     /translation="MSSADFRYQSGFGNDFSSEALPGALPIGQSNPQRPAYGLYTEEI
                     NGTSFTAPRASMRRCWTYRIRPSAVHEPFREIDGRLVRSAPFDEATASPNQLRWRPLP
                     LPKEPTDFVAGLVTMGGNGDPAQQTGAAVHVYAANASMVDRFFYDADGELLIVPQQGA
                     LTVRTELGVLHVPPGEVCVVPRGVKFRVELDGPSRGYVCENYG"
BASE COUNT          480 a         1018 c         1008 g          479 t
ORIGIN      
        1 gcgctcgagc tcggcggcga cgtcggccaa cgcgtcgttg tacgcgctct cgccgcgcac
       61 gtgcgcgggt aacaacccga tgccgagccg ctcgtagacg ctcgcgaaat agcggtacga
      121 caaatcgacg agctgccgcc acagctcgag cgtgcgtgcg tcgcccgctt gcagcagcac
      181 gactcgctgg cgcgcacgat cggcgaacgc agggtcgtcg tcgaacttga cgcgcgctgc
      241 gcgatagaag tcgctgagct cgcccacgct ggcctgctct ccgctcccgc cgacgtcgag
      301 cagatgctcg atcagcatgc cgaacggcgt gccccagtcg ccgatatggt tttgccttac
      361 gacgtcgtga cccgcgaact cgagcaggcg cgcgagcgcg tcgccgatga tcgtcgagcg
      421 caagtggccg acgtgcattt ctttcgcgac gttcggagcc gaatagtcga tgacgacgcg
      481 ctcctggtgc gccgcgagcg ggacgccgag gcgtgcgtcg cgcgcgacgg ccgcaagtgc
      541 gctctcgagg aacgacgcct gcaacgtcaa attgatgaag ccgggcccgg caatctcgac
      601 gcgctcgcaa atgtccgcga gctccgcggc ggcgacgacg gcctcggcga cctggcgcgg
      661 cgggcgcttc aatggcttcg caagacccat cgcgagatcc gcctgaaagt cggctcgctc
      721 cgaacggcgg atcattgcat cgacgttcgc atgctccggg ccgaacgcct tcgcgaccgc
      781 gcgctgcagc cgcgcagcga gggcggcgcg cggaaattgg gcctgcgaag attcgttcgg
      841 gctagacggc acggcggctc tcggccagta aaggccgcgt agtataaggc gcgagagtgc
      901 gcgccgcgca gtttcgccgc agtgaaggag agcaccatgt tgccgagccc cgtgcaggac
      961 ttgaaggcgt tcgtccccgc gcgcgactac acgctggcca aacagttcta tctcgatctc
     1021 ggcttcacgc tcgactggag cagcgaccaa gtcgcggaat tctcagccgg ctcgttccgc
     1081 ttcttgttgc agccgttcta cgtcaaagag cacgccgaga atttcatgat gagcttgaac
     1141 gtcgacgaca ccgaccgctg gtgggagcac atcgagcgca tcgagctcgt cgcaaaatac
     1201 ccgggcatca tggcgcgcgc tcccgcgatg cagccgtggg gcctgcgcgt gctctacgtc
     1261 agcgatccca cgggcgtgct ctggcacatc accgagcggc cgaaggcctg agcggctacg
     1321 gcacgagcac gatcttgccc gccgtctgcc gcgcttccag cgcctcgtgc ccttgcgcgg
     1381 cctcggcgag cggcatgacg cgatcgatcg tgatcgtgag cttgcccgaa gcaagccact
     1441 cgaataggtc gttcgctcgt cccacgagct cgtcgcgcgt cgcgatgtat tgattgagcc
     1501 cggggcgcgt caggaacaac gatcctttgg caccgagcac ggccggatcg aacggcggca
     1561 cggcgccgct cgagaagccg aacagcgcga gcatgccgcg cggccgcagg ctgttcaaac
     1621 tcttgtcgaa cgtgtccttg ccgacggagt cgtagacgac gtcgacgccg cggccgttcg
     1681 tcagcctctt gacctcagcc tcgaagtcct gcgagcgata gtcgatcgtg acggcagcac
     1741 cggctgcgcg cgcgaccgcg gctttggccg cgccgccgac cgtgccgtag accgtggcgc
     1801 cgcgcagccg cgcgacctgc gtgatcagct ggcctgctcc gccggcggcc gcgtgcacga
     1861 gcgcgacatc gccgggtttg atttcgaagg tgctgtgcgt caggtagtgc gccgtcatgc
     1921 cttgcagcat gacggccgcc gccgtctcga gctcgacggc cgcgggcacc ttcacgagcc
     1981 ggcgcgccgg cacgacggcg tactcggcgt acgagccgag caccatcgca taggcgacgc
     2041 gatcgccgac ggcgacttcc gtgacgccgg ggccgacggc cgtcacgacg ccggccgctt
     2101 cggagccgag cgtgaacggc agcgcgggcg gcttgtagcg gcccgtgcgg tgctgcacgt
     2161 cgatgaagtt gacgccggcc gctgcgatct cgacgagcgc ttcgccggct tgcggaacgg
     2221 gcaccgaaac ctcgtcgtag cgcagcgcgt cggggccgcc gcacgcgtgc acgcgtattg
     2281 ctttcatgct gactccttga ttggctcgat tttatatcgc ggccgagcgc cgcggcggtt
     2341 tactatgcgc ggcagtgccc tcgagcccgc acgactgatg agcagcgcag attttcgcta
     2401 tcaatccggc ttcggcaacg acttctcgag cgaggcgctg ccgggcgcac tgccgatcgg
     2461 ccagagcaat ccgcagcggc cggcgtacgg cctgtacacc gaggagatca acggcacgtc
     2521 gttcacggcg ccacgcgcgt cgatgcggcg gtgctggact tatcgaatcc ggccgtcggc
     2581 cgtgcatgag ccgtttcgcg aaatcgatgg ccgtctcgtg cgcagcgcgc cgttcgacga
     2641 ggcaaccgcg tcgccgaatc agctgcgctg gcggccgctg ccgctgccaa aggagccgac
     2701 cgacttcgtc gcgggcctcg tgacgatggg cggcaacggc gatccggcgc agcaaaccgg
     2761 cgccgcggtg cacgtctatg cggccaacgc atcgatggtc gaccgcttct tctacgacgc
     2821 cgacggcgag ctgctgatcg tgccgcagca aggcgcactg accgtgcgca cggagctcgg
     2881 cgtgctgcac gtgcctcccg gggaagtctg cgtcgtgccc cgcggcgtga aatttcgcgt
     2941 cgagctcgac gggccgtcgc gcggctacgt ctgcgagaac tacgg
//
LOCUS       DATLEE010000098         6200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8252147, whole genome shotgun sequence.
ACCESSION   DATLEE010000098 DATLEE010000000
VERSION     DATLEE010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8252147"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VM692_02120"
     CDS             complement(<1..954)
                     /locus_tag="VM692_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ50987.1"
                     /translation="MTRRYMALAITIAAILGAHPTFAQNKFVPVTDAMLKNPDPADWL
                     MWRRTLDSWGYSPLDDIDRSNVAGLTLAWSVDLDEAPSQEGIPLVYDGVLYFPSPMDV
                     TTAFDAATGKKLWEHRRALPADLGTFVPFPQTNRNLAIYGRLIIDNGADDFVYALDAV
                     TGKMVWETKILDYKTYHVKQGSAPLVANGVLIAGRNCQPNGGPDACVITGHDARTGKE
                     LWRKRTIPKPGEPGGDSWGDVPDDKRWHVGAWMIPSYDAQLDLVYVGTSVTAPAPKFM
                     LAGNDKQYLYHNSTLALRPKTGETVWYYQHAVDHWDLDHPFE"
     gene            complement(1034..2089)
                     /locus_tag="VM692_02125"
     CDS             complement(1034..2089)
                     /locus_tag="VM692_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUQ50988.1"
                     /translation="MTLAPDPANATDEARLRQRIAAAGLVPLWTFFKDWFAAEPHAGA
                     CPHLWRYADLRPLIMEAGALVSTAEAERRVLVLENPGLVGRHLATDALYAGLQLILPG
                     EFARAHRHTAAALRFIVEGDDTYTAVAGERCYMRPGDFIVTPSWTWHEHRNEGQEPTI
                     WLDVLDVPLIRFLGAGFSEHYPEPTFPTAVPPGDSRLRFGRNLLPVGYRRGAGASPVF
                     AYPFDYAYEALEHLRAHSDWDVCHGVKMEYIDPTTGGPAIPTISTFLQLLPQDFKTAS
                     YRATPSSVTVVVRGRGCVTIGEGADARTFDYAPKDLWTVPSWQPVSIAANEESVLFYA
                     SDEAVHRKLGVWREQRS"
     gene            2197..2781
                     /locus_tag="VM692_02130"
     CDS             2197..2781
                     /locus_tag="VM692_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50989.1"
                     /translation="MTSRREFLKVGLAAAALPAAAQTELADEGTPTVALYKVLYDTRF
                     PASVAFGRRASARGLVVQAMAGDMTGFWYDDLYHRWRNGPAAIAGLTGRGALFCLERL
                     AWDQRMRVVFRGEHTAGADACVAHRFEGPATLLPTAVRAAASSAWADALADVVATCPS
                     SRRTHDTATALTASARTLPTEPLFSWIIAPVARA"
     gene            2790..3125
                     /locus_tag="VM692_02135"
     CDS             2790..3125
                     /locus_tag="VM692_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUQ50990.1"
                     /translation="MTSKSLWVAGLALSFAAGVQAQEAGARGNEVFQYWCAPCHAPGP
                     RHPGTQALESLYKGAKPAALEQRTDLVPELTRSFVRTGVSVMPPFRKTEISDADLAAL
                     AAYLAPKAR"
     gene            3198..4049
                     /locus_tag="VM692_02140"
     CDS             3198..4049
                     /locus_tag="VM692_02140"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HUQ50991.1"
                     /translation="MITLRVGGVPEHFNLPWHLALERRSFEPLGVELQWHDYPGGSGA
                     MAKAVRAGELDVALLLTEGAVAAIADGVAMKVASLYTESPLVWGIHVPAASRFRAVAE
                     LRGARDAISRAGSGSQLMAFVHARSQGWPVDRLEFVVVGNLAGAADAFAAGSADVFFW
                     EKLMTKPLVDAGRFRRVAELTAPWPAFVVCVADALRGAPRAAVARALGVVLHEAQALR
                     SRPDAAALIAARYGLDARDVGQWLETTRWSTRVGVAQADLESACTALSSLGVLTRAIH
                     AAECIGG"
     gene            complement(4147..4533)
                     /locus_tag="VM692_02145"
     CDS             complement(4147..4533)
                     /locus_tag="VM692_02145"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ50992.1"
                     /translation="MRTVLACALGLATLLSAQAFAHHSAVMFDDKKEVTVTGTVKEFQ
                     YTNPHSWLLVDVKNADGSVTTWGFEAEGPSTLTRAGIRKSDFPFGTEITITGNPMRDG
                     RPAAAWVRAKRGDGKDFDPRAGFAIK"
     gene            complement(4546..5442)
                     /locus_tag="VM692_02150"
     CDS             complement(4546..5442)
                     /locus_tag="VM692_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ50993.1"
                     /translation="MIARILAAAAVAATALALTTAAAQEAAPPSAEKQQMTALGVEHK
                     TARALYDYFKQQANNGKALTWRSVPDWTGIWTREASPFFWDPDQASTTALPTAKLTPE
                     HERLLRDKLDKINRGIEFDPLSNCEPAGMPRWLVEPFLKEFVVTPSETYLINEMQNEM
                     RRVYTDGRDHPSEADAYPLWEGDSIGFWDGDKLVIHTSQMRAGQYQRIQPFYTEQVET
                     VEIWQKTDPTTMVVHVWAFDPPALAEPWYTRQVYKQLSNDDASLRIRYWNCGENQNNA
                     VEKTDTGTSDFSNFTFTDKDDQ"
     gene            complement(5547..5951)
                     /locus_tag="VM692_02155"
     CDS             complement(5547..5951)
                     /locus_tag="VM692_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUQ50994.1"
                     /translation="MNHRRLQILVASSALATAALIGAVSAQQASQAVDGDATRGKPLF
                     SDTFNCYACHGFDAQSGERRLVPLNYTQDGFITFVQNSPLPNMPAFPDATPQQLADIY
                     AYIRTIPADAPAVSDLPLLRDILDRKARAAAD"
     gene            complement(5963..>6200)
                     /locus_tag="VM692_02160"
     CDS             complement(5963..>6200)
                     /locus_tag="VM692_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUQ50995.1"
                     /translation="VMLVQRTAARRGRGNSNVDIGNVIRDPAPDYAKIAQGFGVYAEG
                     PIADPAALAPALERALARVRAGEPALLDVISQPR"
BASE COUNT          937 a         2011 c         2134 g         1118 t
ORIGIN      
        1 ctcgaacgga tgatcgaggt cccagtgatc aacggcgtgc tggtaatacc acaccgtttc
       61 gccggtcttc ggccgcagcg cgagcgtcga gttgtgatag aggtattgct tgtcgttgcc
      121 ggccagcatg aatttcggcg ccggcgccgt gacggacgtg ccgacgtaga cgagatcgag
      181 ctgcgcgtcg tagctcggaa tcatccacgc gcccacgtgc cagcgcttgt cgtcgggcac
      241 gtcgccccag ctgtcgccgc cgggctcgcc cggcttcggg atcgtgcgct tgcgccacag
      301 ctccttgccc gtgcgcgcgt catggcccgt gatcacgcag gcgtcgggcc cgccgttcgg
      361 ctggcaattg cggcccgcga tcagcacgcc gttggcgacg agcggcgccg agccctgttt
      421 aacgtgatac gtcttgtagt cgaggatctt ggtttcccaa accattttgc cggtgacggc
      481 atcgagcgcg tagacgaagt cgtcggcgcc gttgtcgatg atgagccggc cgtagatcgc
      541 gagattgcga ttggtctgcg ggaagggcac gaacgtgccg aggtccgccg gcagggcgcg
      601 ccggtgctcc cagagcttct tgcccgtcgc ggcgtcgaac gccgtcgtaa cgtccatcgg
      661 gctcggaaag tacagcacgc cgtcatagac gagcggaatg ccctcctgcg acggggcttc
      721 gtcgagatcg acggaccatg cgagcgtgag cccggccacg ttgctgcggt cgatgtcgtc
      781 gagcgggctg tagccccagc tgtcgagcgt gcgccgccac atcagccagt cggctgggtc
      841 cgggttcttc agcatggcgt ccgtgacggg cacgaacttg ttctgcgcga acgtcgggtg
      901 ggccccgagg atcgccgcaa ttgtgatcgc gagagccatg tatcgccgtg tcatttcgag
      961 atccttcgag tcattcgcct tcgattcgac cgagcacgtc gacgacctcc ggttgccgca
     1021 gcacggatgt tagctagctg cgctgctcgc gccaaacgcc cagcttgcga tgcacggctt
     1081 cgtcggatgc gtagaacagc accgactctt cgttcgccgc aatgctgacg ggctgccagc
     1141 tcggcacggt ccagaggtct ttgggcgcgt agtcgaacgt gcgcgcgtcg gcgccttcgc
     1201 cgatcgtcac gcacccgcgg ccgcgcacga cgacggtgac gctgctcggc gtcgctcgat
     1261 aactcgccgt cttgaaatct tgcggcagca gctgcaagaa cgtcgagatc gtcggaatcg
     1321 cgggcccgcc cgtcgtcgga tcgatgtact ccatcttgac gccatggcag acgtcccagt
     1381 cgctgtgcgc gcgcagatgc tcgagcgctt cgtaggcgta gtcgaatgga taggcgaata
     1441 cgggcgatgc gccggcgccg cggcggtagc cgacaggcag cagattacgc ccgaagcgca
     1501 gccggctgtc gccgggcggc acggcagtcg ggaacgtcgg ctcgggatag tgctccgaaa
     1561 aaccggcgcc caagaagcgg atcaacggca cgtcgaggac gtcgagccaa atcgtcggct
     1621 cttggccttc gttgcggtgc tcgtgccagg tccacgacgg cgtgacgatg aaatcgccgg
     1681 ggcgcatgta gcagcgctcg ccggcgacgg ccgtataagt gtcgtcacct tcgacgatga
     1741 aacgcagcgc ggcggccgta tggcgatgcg cgcgagcgaa ctcgccgggc aggatcagct
     1801 gcaagccggc atagagagcg tcggtcgcga gatggcgccc caccagtccc ggattctcga
     1861 gcactagcac gcggcgctcg gcttcggccg tcgacaccag cgcgccggcc tccatgatca
     1921 gcggccgcag gtcggcatag cgccagagat gcgggcacgc gcccgcgtgc ggctcggcag
     1981 cgaaccaatc cttgaagaac gtccagagcg gcacgaggcc ggcggcggcg attcgttgcc
     2041 gcaggcgggc ttcgtccgtc gcattggcag gatcgggcgc gagcgtcatg aagggcgagt
     2101 gtagcagccg gccgccggcc ggcccgccga agtcgattcg ccgcgccgcg gcggcgtatc
     2161 atacggacgc cgtacgttgc cacggcgagg gcgttgatga cgagccgcag agagttcctg
     2221 aaggtcggat tggcggccgc cgcgctaccc gcggccgcgc aaacggagct cgctgacgag
     2281 ggaacgccga ccgtcgcgct atacaaggtt ctctacgaca cgcgctttcc ggccagcgtc
     2341 gcattcgggc gccgagcttc ggctcggggt ctcgtcgtcc aagccatggc gggggacatg
     2401 acggggttct ggtacgacga tctctaccat cgttggcgaa atgggcccgc cgcgatcgcg
     2461 gggctcacag gacgcggcgc gttgttttgc ttggaacggc tcgcctggga tcaacgcatg
     2521 cgagtcgtgt ttcgcggcga gcatacggcg ggcgccgacg cgtgcgtggc gcatcggttc
     2581 gaaggaccgg ccacgctgct gccgacggcg gtgcgcgccg cagcgagctc ggcttgggcc
     2641 gacgcgttgg ccgacgtcgt cgctacctgt ccgtcgagcc gtaggacgca cgacactgca
     2701 acggcgctga ccgcgagcgc gcgtacgttg ccaaccgagc cgttgttctc gtggatcatc
     2761 gcgcccgtag cgcgtgcgta agggtgccga tgactagcaa gtcgctgtgg gtggccggcc
     2821 tcgcgctctc gttcgccgcc ggcgtgcagg cgcaagaggc cggcgcgcgc ggcaacgagg
     2881 tctttcagta ttggtgcgcg ccgtgtcatg caccgggccc gcggcatccg ggcacgcaag
     2941 cgctcgagtc gctctacaaa ggcgcgaagc cggcggcgct cgagcaacgc acggacctcg
     3001 tgccggagct cacgcggtcg ttcgtgcgta cgggcgtgtc ggtcatgccg ccgtttcgca
     3061 agaccgagat cagcgacgcc gatctcgcgg cgcttgccgc ttacctggcg ccgaaggctc
     3121 gttagcgggg ctcgcgaacc catggcgggc ggcggcactt gggctgctct ttcactctat
     3181 aaagcgctag caagtatatg attaccctca gggtcggggg cgtccccgag cacttcaatc
     3241 tgccatggca tctggcactc gagcggcgca gcttcgagcc gctcggcgtg gagctccaat
     3301 ggcacgacta tccaggcggc tccggcgcca tggcgaaagc cgtgcgcgcg ggcgagctcg
     3361 acgtcgcgtt attgctgacc gagggtgccg tcgccgcgat cgccgacggc gtcgccatga
     3421 aggtcgcgag cctctacacc gagtcgccgc tcgtctgggg aattcacgtg ccggcagcct
     3481 cgcgctttcg agccgtggcc gagctgcgcg gcgctcgcga cgcgatcagc cgggcggggt
     3541 ccggatcgca gctcatggcg ttcgtgcacg cgcggtcgca gggttggccc gtcgaccggc
     3601 tcgagttcgt cgtggtcggc aatctggccg gtgccgccga cgcgtttgcg gccggcagcg
     3661 ccgacgtctt tttctgggaa aagctcatga cgaagccgct cgtcgacgcg gggcggtttc
     3721 gccgcgtcgc cgagctcacg gcgccgtggc ccgcgttcgt cgtctgtgtt gccgacgcgc
     3781 ttcgcggggc gccgcgcgcg gccgtcgctc gggcgttggg cgtcgtcttg cacgaagcgc
     3841 aagcgctgcg ctcgcgaccc gacgccgcgg ctttgatcgc cgctcgctat ggtctcgacg
     3901 cgcgcgacgt cgggcagtgg ctcgagacga cgcgttggag cacgcgcgtc ggtgtcgcgc
     3961 aggcggacct cgagtcggca tgcacggcgc tgagctcgct cggtgtattg acgcgcgcca
     4021 tccatgccgc cgagtgcatc ggcggctgag atccgtaacc gtgctcgggc gcgttgagct
     4081 caaatagaaa ggcccgagcc gctcgacggc tcgggccttg tcgttcgaga aggtctagcg
     4141 cgatccctac ttgatcgcga aacccgcgcg cggatcgaag tccttgccgt cgccgcgttt
     4201 cgctcgcacc caggccgcgg ctggtcggcc atcgcgcatc gggttgcccg tgatcgtgat
     4261 ttccgtgccg aacgggaagt cgctcttgcg gatgccggcg cgcgtcagcg tgctcgggcc
     4321 ttctgcctcg aagccccatg tcgtgacgct gccgtccgcg ttcttcacgt cgacgagcag
     4381 ccacgaatgc gggttcgtgt actggaattc cttgacggtg ccggtgaccg taacttcctt
     4441 cttgtcatcg aacatgaccg ccgagtgatg cgcgaaagct tgtgcgctga gcaaagttgc
     4501 gagcccgagc gcgcacgcca aaactgtcct catggccaac tctccttact gatcgtcttt
     4561 atctgtgaac gtgaagttcg agaaatcgga agtgccggta tccgtcttct cgacggcgtt
     4621 gttctggttc tcgccgcagt tccagtacct aatacgcaag ctcgcgtcgt cgttcgacaa
     4681 ttgcttgtag acctgcctag tgtaccaggg ctccgccagg gccggcgggt cgaacgccca
     4741 gacgtgcacg accatcgtcg ttgggtccgt cttctgccaa atctcgacgg tctcgacttg
     4801 ctccgtataa aacggctgga tgcgttgata ctggcccgcg cgcatttggc tcgtgtgtat
     4861 gacgagcttg tcgccgtccc aaaagccgat cgagtcgccc tcccagagcg gataggcgtc
     4921 tgcttcgctt ggatggtcgc ggccatccgt gtagacacgg cgcatctcgt tctgcatctc
     4981 gttgatgaga tacgtctcgc tcggcgtgac gacgaactcc ttcaagaacg gctcgacgag
     5041 ccaccgtggc atgccggcgg gctcgcagtt gctcagcggg tcgaactcga tgccgcggtt
     5101 gatcttgtcg agcttgtcgc gcaacaggcg ctcgtgctcc ggcgtgagct tcgcagtcgg
     5161 cagggcggtc gtcgacgcct gatcggggtc ccagaaaaac gggctcgctt cgcgcgtcca
     5221 gatgccggtc caatccggca cggagcgcca ggtcaacgct ttgccgttgt tcgcttgctg
     5281 tttgaaataa tcgtagagcg cacgcgccgt tttgtgctcg acgccgaggg ccgtcatttg
     5341 ctgcttctcc gccgacggcg gggccgcttc ctgcgctgcg gcagtcgtca gcgccagtgc
     5401 cgtcgccgcg acggccgcgg cggccaggat ccttgcgatc atcgaacagg gtctccgcta
     5461 gcgggccgag gcctattcta gtgcgccggc cgtgcgccgg catccgccgc cgccaaggca
     5521 gcaggcgtaa ggagctcgaa gcagcactag tcggccgcgg cgcgggcttt ccggtccagt
     5581 atgtcgcgca gcagcggcag gtcgctgacc gcgggggcat cggccgggat cgtgcggatg
     5641 taggcgtaga tgtcggcgag ctgctgcggc gtcgcgtccg ggaaggccgg catgttcggc
     5701 agcggcgagt tctgcacgaa cgtgatgaac ccgtcctgcg tgtagttcag cggcaccaac
     5761 cgccgctcgc cgctctgcgc gtcgaagccg tgacaggcat agcaattgaa cgtgtccgag
     5821 aacaacggct tgccgcgcgt cgcgtcgccg tcgacagctt ggctcgcctg ctgggccgac
     5881 acggcgccga tcagcgcggc cgtcgcgagc gccgaggacg cgaccagtat ttgcagtctg
     5941 cggtggttca ttgcctcgtc cttcagcgcg gctgcgagat cacgtcgagc agggccggct
     6001 cgccggcccg aacgcgcgcg agcgcgcgct cgagtgccgg tgccaacgcg gccggatccg
     6061 caatcgggcc ttcggcgtag acgccgaagc cttgcgcgat cttcgcgtag tccggcgccg
     6121 ggtcgcgaat gacgttgccg atgtcgacgt tggaattgcc gcggccacgc cgtgcggccg
     6181 tgcgctgcac gagcatgact
//
LOCUS       DATLEE010000099        10940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6017801, whole genome shotgun sequence.
ACCESSION   DATLEE010000099 DATLEE010000000
VERSION     DATLEE010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6017801"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="gshB"
                     /locus_tag="VM692_02165"
     CDS             complement(<1..842)
                     /gene="gshB"
                     /locus_tag="VM692_02165"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HUQ50996.1"
                     /translation="MSQSRRTLGVVMDPIDTIKPKKDSTLAMLLAAQRRGWEIVYLRQ
                     QDLEIRGGAPVGHGARLKVRDDTERWFEIGVPWSDGLEKLDALLMRKDPPFDMEYIYT
                     TYILELAENRGLLVVNKPASLRDINEKAYTSWFPQCTPPSLVTRSRTALLEFLKEHGK
                     VVLKPFDGMGGRSIFIVTAGDLNTNVIIETLTDDGRRFTLAQRYIPEIVQGDKRVLLI
                     DGEPIDYALARIPAPGESRGNLVMGARGEGRPLTERDRWLCAQVGPTLAAKGVLFAGL
                     DVIG"
     gene            1089..1496
                     /locus_tag="VM692_02170"
     CDS             1089..1496
                     /locus_tag="VM692_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUQ50997.1"
                     /translation="MSGSSAVNPRNDLQGLKILVIDDSKTIRRTAETLLAKEGCEVFT
                     AVDGFDALSKIADHRPDIIFVDIMMPRLDGYQTCSLIKHNKVFKDTPVIMLSSKDGLF
                     DRARGRLVGSEQYLTKPFTKDELLGAIHSHVAK"
     gene            1517..1894
                     /locus_tag="VM692_02175"
     CDS             1517..1894
                     /locus_tag="VM692_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUQ50998.1"
                     /translation="MALIMIVDDSPTEVHVLKTALEKHGFDTVSASDGSECLSLAREV
                     RPDLIFMDVVMPRVNGYQATRTLTRDPQTKSIPVVMITTKDQETDRIWGMRQGAVDYL
                     VKPVTEAVLVAKAQEVLERGSSA"
     gene            1891..2454
                     /locus_tag="VM692_02180"
     CDS             1891..2454
                     /locus_tag="VM692_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HUQ50999.1"
                     /translation="MNLHALAEQPFELLVELERRARAAIAAREGTPATSDEWVGIGFR
                     LGAERFVTGRADVREVLPIPEQVTRVPGAKPWLRGIANLRGQLLTVVDLKSFLGAGTP
                     AADRQARVLVVASREVPTGLIVDEVVGFRRFGAADYREEAGPSVIRCEHYLEGSYRRG
                     TDVWPRFSLLKLLEDPQFLTAGETVRA"
     gene            2459..4564
                     /locus_tag="VM692_02185"
     CDS             2459..4564
                     /locus_tag="VM692_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HUQ51000.1"
                     /translation="MTKSSPWPKMLPSLGILLTIALLAAAGSVWWSGSGTTTISDGGL
                     GELIAITQAARTETTAALSGAEASFESVAESHAAVTRLRGTVAGNQAASADARALAAD
                     GPLWQRIERNLDAVVESRARLADLETARAEVQELVPKLLVAAGNIASALPAADLMADQ
                     PYLQRFELTVQSLQQQVRSLGPGAAVDDAVRRMQDAEQYLSQFVRGLRGEDTTLGVMA
                     VPERTAQADLAAMDDLLERARRSIAAIGSAAEPLKTAVAAAKEYDAAAAELLAQYRAT
                     DVSIAATTAGGLGARLPLLLVFAALAIAALMMFVYYRGRDFRHSAEAQAEQNERNQQA
                     ILRLLDELSSLADGDLTVQATVTEDITGAIADSINYAIEALRELVATINDSSILVDAA
                     SKQTEGTARHLVRSAETQAKQAAAASESMARMAISIEEVSGNAERCSDVARHSVEIAH
                     KGGEAVRRTIAGMNTIRETIQDTSKRIKRLGESSQEIGNIVELIEEIAEQTNILALNA
                     SIEASRAGEASRGFAVVADEVQKLAERSAGATKKIEVLVSTIQSDTNEAVMSMERSTT
                     DVVGGALLAENAGAALEEIEQVSHQIASLVHNISSSSKEQTSVAGAITKNMHVLRDIS
                     SKTTESTTAASSAISKLSELASQLRRSVSGFTLPDQGSGTGVLSAAGVAASLARAEKT
                     PPPPPPPVDPVVERRRQSG"
     gene            4574..9961
                     /locus_tag="VM692_02190"
     CDS             4574..9961
                     /locus_tag="VM692_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HUQ51001.1"
                     /translation="MATQPMDDLAAHAFELVNRELASTLDAARSQLEDYVDGRAAADA
                     LLRTAEHLHTARGALKIVEIHGAALLAEEMEQTCRRLAEAKDDDKSEQGIEALTRAMV
                     QLPAYLERLLSGGKDVALALLPLLNDLRQARDKPSLSEGTLVLLNSGPFERTVAPRTG
                     AASADVGRGFDKLAQRLRPPFQAALLGWIKGAEPARFLDELLRVCTNLERSAASEQVK
                     QLWTVMTAVLTAIRSGGLEASVTLKRLIGQVDRQLKRLIDGGESALVNAPPVELLNSL
                     LYYVAKANSKDPRIEALRKQYNLSEMLPGQAQVEQAREGLSGPSVKLMRTVAQAIKED
                     LGTVKDALDIFVRTGMQGVDKLSPQVDMLKKIGDTLGVLGLDKARGQIQRETQELAAI
                     VLNGKVLDQSGLEKIAATLLDVEDTLDRELVRAVMPGDESEAPEATGEADTQQRQVTQ
                     AVMGECTVNLAKVKEAVIQLVDHPTDTRPLEQAKPQLRGITAGLLMLNKTKAVSVVER
                     VGSVIATRLAAGTKLRPEYLERLADAIVSVEYYMETVSAGRSDPWYMLENADRCLDLL
                     ETLPVAKPAAPTAAGGAAPPPRPAASKPAAPPPRVMQVEEGRSDPELVEVFIEEAKEE
                     IENIRRNLPAWAADRTNSEALIATRRSFHTLKGSGRMVGAQLIGEFAWSIENLLNRLI
                     NQTLEPTPNMIAFISSASGALPALLEQLEIGLPPKIDVQLLMKQAEAFADGDPEAASL
                     TSQSLRTPALPAAAEAAAPATSEPGMDPVLVDIFVKEMRGHLEVIRRFLAGATPGGGP
                     HTVEEPLFRACHTLLGSARMAGFEPAMQVAAPVAEHMRKYFDSGIGVPDSGVAALRVA
                     ADQVDAMANALAAGRTVVVDPATLKALDGLQFREPASASPAAPLPSASAPQVPPPVEQ
                     AAAAPPVEREAEPEAAASRFDPEIAAIFAEEAAEILDNSQAALQEVRQRQDQAAVAML
                     QRYLHTLKGGARMAGVLPMGDLSHALETLLSRIAEGRSRPTPAALELVQRGLDELQQM
                     RDAIDAGRAVAAASELVARLEAFEAPVVAESVPPAPSTSTPVQPAVAAVAEIVAHEPP
                     AATAPGAPPETVPIAELAGADFEQLESIELTPASVPPADARAAAEVIEEPTVDQPVSF
                     EEPVAEPTIVEPKVELAVPPAAVAQPVEPAPVAVAPAPAQVEQRSAERAETARVDAGL
                     LDALLNGAGEINIFQSRLSQQLHSIEFHLGELGGTVSRLREQLRKLEAETEAQILHRH
                     QDDAEIESGFDPLELDRYSTIQQLSRALAESANDVASINELLHGLTNEADTLLTQQAR
                     VTSDLQSGLMQTRMVPFQRHVPRLQRIVRQACADTGKQAELVVDGEGNEIDRQVLESM
                     LPPFEHLLRNAIAHGIEKPAVRQQRGKPEIGQVLLKIRREGSEVIIEVGDDGGGLDLA
                     AIRRKGYEKGLLAENQRITDEQAMELILQPGFSTASELTQAAGRGVGMDVVDNEIKKL
                     GGSMRIETRTGEGTRFVIRLPYTLAITHALIVNVGEETFALPLPTVEGITRLSRDKIL
                     KHLTEDEPKLDYGGIVYRIQHLGSLVGAAPSALPEDENAVSLVLIRAGDNSTALLMDS
                     LEGSREIVVKTLGPHIASVPGVTGATILGDGRVIMILDPGTLVRSQRPADVVRAAPKP
                     TASTQLTALVVDDSITMRRVTQRLLERRGAKVHTARDGLDAITVLQEHTVDIILLDIE
                     MPRMDGYQFATHVRNDPKLKQLPIIMITSRSGEKHRAKAIEIGVNDYLSKPYQESQLT
                     AAIGALLGREL"
     gene            9966..10436
                     /locus_tag="VM692_02195"
     CDS             9966..10436
                     /locus_tag="VM692_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HUQ51002.1"
                     /translation="MEQKELYSLLIPLQNERLLVPRMCVAEVIAFADTARSRDDAAPD
                     WYLGAIEWNNRRVPVVSFDAPKGDERKSKRSRTRVVVFHAITQELKGGYYGILTQGFP
                     QLVRVNPDVLGLETEQPLPEFFPAICRARMIHEYPLVPDVERLEKMIAELPLTA"
     gene            complement(10453..>10940)
                     /locus_tag="VM692_02200"
     CDS             complement(10453..>10940)
                     /locus_tag="VM692_02200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HUQ51003.1"
                     /translation="MQALPKSDAMDLIVQKATELGVRALVPVYTEFSVVKLDEERTAR
                     RVDHWRKIAQSACEQCGRHRPPSIEPPATLVDAFAALPAGVARVALDPKAEHAFGSQA
                     LPEPALIVAVGPEGGFGPADWRRLDAAEFARMTLGRRVLRAETAALAVCAIAQSRWGD
                     L"
BASE COUNT         1945 a         3647 c         3679 g         1669 t
ORIGIN      
        1 tcgccgatga cgtcgagccc cgcgaagagc acgcctttcg ctgccaacgt agggccgact
       61 tgcgcgcaga gccagcgatc gcgctcggtg agcggccggc cctcgccccg cgcgcccatg
      121 acgaggttgc cgcggctctc gcccggcgcc gggatgcgcg cgagagcata gtcgatcggc
      181 tcgccatcga tcagcagcac gcgcttgtcg ccctgcacga tctcggggat atagcgctga
      241 gcgagcgtga aacgccggcc gtcgtccgtc agcgtctcga tgatgacgtt ggtgttcagg
      301 tcgccggccg tcacgatgaa gatcgaccgc ccacccatgc cgtcgaacgg cttcagcacg
      361 actttcccgt gctccttgag gaactcgagc aacgccgtac gcgagcgcgt gacgagactc
      421 ggcggcgtgc attgcgggaa ccaggacgtg tacgccttct cgttgatgtc gcgcaggctt
      481 gccggcttgt tgacgacgag caggccgcga ttttcggcaa gctcgaggat gtacgtcgtg
      541 tagatgtact ccatgtcgaa cggcgggtcc ttgcgcatca gcagggcgtc gagcttctcg
      601 agcccgtcgc tccagggcac gccgatctcg aaccagcgct cggtatcgtc gcgcactttg
      661 agccgggccc catgcccgac cggcgcgccg ccgcggatct ccaagtcttg ctggcgtaaa
      721 tagacgatct cccaaccgcg acgctgggcg gccaacagca tcgccagggt cgaatctttt
      781 ttgggcttga tcgtgtcgat cgggtccatc acgaccccga gcgttcgccg ggactggctc
      841 atgaaaacat tcctcgcttt tgcgcgcggg ttcgcagttt cgcgaaaaag cgcaacgcta
      901 ccataacccg attctgtcga acgttttgcc ggcgcgacgc gcttcgagca gcgattgcga
      961 gtgccataaa gtgagtgcgc cggtcgatgg aaggcatggc gccaatatgt taaaaggcgc
     1021 ggtgagtccg aggtgcctgc tcggctccgg ctcccactaa cgccgagcga cctgcgggag
     1081 ggaatcaagt gtctggcagt agtgcagtga acccgaggaa cgacttgcag gggctcaaaa
     1141 ttctcgtcat cgacgacagt aagacgatcc gcagaactgc cgaaacgttg ctcgccaagg
     1201 aaggctgcga agtgtttacc gccgtcgatg gcttcgatgc gctttcgaag atcgccgatc
     1261 atcggccgga cattattttc gtcgacatca tgatgccgcg cctcgacggt tatcagacgt
     1321 gctcgttgat caagcacaac aaagttttca aggacacgcc cgtcatcatg ctgtcgagca
     1381 aggacgggtt gttcgaccgc gctcgcggcc gcctcgtcgg ctcggagcag tacctgacga
     1441 aaccgttcac caaagacgag cttctcggcg ccatccatag ccacgtggcc aagtagacgg
     1501 atcgcaagga tcgcctatgg ctttgatcat gatcgtcgat gactcgccca cggaggtgca
     1561 cgtcctgaag acggccctcg agaagcatgg cttcgacacc gtgtcggcgt ccgatggctc
     1621 ggagtgcttg agcctcgcgc gcgaggtgcg gccggacctg atcttcatgg acgtggtgat
     1681 gccgcgagtc aacggctacc aggcgacgcg cacgctgacg cgcgatccgc agaccaagtc
     1741 gatccccgtc gtgatgatca cgaccaaaga tcaggagacc gaccgcatct ggggcatgcg
     1801 ccaaggcgcc gtcgactatc tggtcaagcc ggtcacggaa gccgtgctcg tagcgaaggc
     1861 acaggaagtg ctggagcggg ggtcgagcgc ttgaacctgc acgcgctggc agagcaaccg
     1921 ttcgagctgc tcgtcgagct cgagcgccgc gcgcgcgccg cgatcgccgc gcgcgagggc
     1981 acgccggcga cgtcggacga atgggtcgga atcggctttc gactcggtgc cgagcgcttc
     2041 gtgaccggcc gcgccgacgt gcgcgaggtg ttgccgatcc cggagcaagt cacgcgtgtg
     2101 ccgggagcga agccctggct gcgcggcatc gcgaaccttc gcggtcagct gctcaccgtg
     2161 gtcgatttga agtcgttcct gggggccggc acgccggcgg ccgaccgcca agcccgcgtg
     2221 ctggtagtcg ccagccgcga ggtccccacg gggctcattg tcgacgaggt cgtcggcttt
     2281 cgccgcttcg gcgccgccga ctatcgcgag gaagccggcc cgagcgtcat tcgctgcgag
     2341 cactatctcg aagggagcta tcgtcgcgga accgacgtct ggccccggtt cagcctgctg
     2401 aagctactcg aggacccgca gttcctgacg gccggcgaga cggtgagggc gtgagggcat
     2461 gaccaagtcg agcccgtggc caaagatgtt gccatcgctc ggcatcctgc tcacaattgc
     2521 gttgctcgcg gccgcaggct ccgtctggtg gagcggcagc ggaacgacca cgatcagcga
     2581 tggcggtctc ggcgagctga tcgccatcac gcaggcggcc cgaacggaga cgacagcggc
     2641 cctgtccgga gcggaggcct cgttcgagtc ggtggccgag agccacgccg cggtcacgcg
     2701 cttacgcggc accgtcgccg gcaatcaagc cgcgagcgcc gatgcacgcg cgctcgccgc
     2761 cgacggtccg ctctggcagc gcatcgagcg caacctcgac gccgtcgtcg agtcgcgggc
     2821 ccggctcgcc gacctcgaga cggcgcgcgc cgaagtgcaa gagctagtcc ccaagctgct
     2881 cgtcgcggcg ggcaatatcg cgagcgcctt gccggcggcc gacctcatgg ccgatcaacc
     2941 ctatctgcag cggttcgagt tgaccgtcca atcgttacag cagcaagtgc gctcgctggg
     3001 ccccggcgcc gccgtcgatg acgccgtgcg ccgcatgcaa gacgcggaac agtatctgag
     3061 ccagttcgtc cgcggcctgc gcggcgaaga cacgacgctc ggcgtcatgg cggttcccga
     3121 gcgcaccgca caggcagatc ttgcggcgat ggacgacttg ctagagcgcg cgcggcgctc
     3181 gatcgccgcc atcggctcgg cggccgagcc gctgaagacg gccgtcgctg cggccaaaga
     3241 gtacgatgcg gccgccgccg agctgctcgc gcagtaccgc gccacggatg tttcgatcgc
     3301 tgccaccacg gctggcggcc tcggtgcacg gctgccgttg ttgctggtgt tcgctgcctt
     3361 ggcgatcgcg gcgctgatga tgttcgtcta ctaccgcggc cgcgacttcc gccactcggc
     3421 agaggcgcaa gccgagcaga acgagcgcaa tcagcaggcg atcttgcgcc tgctcgacga
     3481 gctgtcgagc ctcgccgacg gcgatctcac cgtgcaggcc accgtcaccg aggacatcac
     3541 gggcgcgatc gcggactcca tcaactacgc gatcgaggcg ttgcgcgagc tcgtggcgac
     3601 gatcaacgac tcctcgatcc tcgtcgatgc cgcgtcgaaa cagacggaag gcacggcgcg
     3661 ccacctcgtg cgctcggccg agacgcaagc gaagcaagcc gccgcggcgt cggagtcgat
     3721 ggcccgcatg gcgatctcca tcgaggaggt ctccggcaac gcggagcgct gctcggacgt
     3781 cgcgcggcat tcggtcgaga tcgcgcacaa aggcggcgag gccgtgcgac gcacgatcgc
     3841 aggcatgaac acgattcgcg agacgattca ggatacgagc aagcgcatca agcggctcgg
     3901 cgagagctcg caagagatcg gcaacatcgt cgagctcatc gaggagatcg ccgagcagac
     3961 gaacattctg gcgctgaacg cgtcgatcga ggcgtcgcgc gccggcgagg cgagccgcgg
     4021 attcgcggtc gtcgccgacg aagtgcagaa gctggccgaa cgctcggccg gcgcgaccaa
     4081 gaagatcgag gtgctcgtca gcacgatcca atcggatacc aacgaggccg tgatgtcgat
     4141 ggagcgcagc accaccgacg tcgtcggcgg cgcattgctg gccgagaacg ccggcgcggc
     4201 gctcgaggag atcgagcagg tttcgcacca gatcgcgagc ctcgtgcaca acatctcgag
     4261 ctcttcgaag gagcagacca gcgtcgccgg cgcgatcacg aagaacatgc acgtcctgcg
     4321 cgacatcagc agcaagacga cggaatcgac gacggcggct tcgagcgcca tcagcaaact
     4381 ctcggagctc gcttcgcagc tgcgccgcag cgtctcaggg ttcactctgc cggatcaggg
     4441 cagcggcacg ggcgtgctca gcgcggccgg cgtcgccgcg agcctcgcgc gcgccgagaa
     4501 gacgcccccg ccgccaccgc cacccgtcga ccccgttgtc gagcgacgcc gccagtccgg
     4561 ttaacgacgg ctcatggcga ctcaaccgat ggatgatcta gcggcgcacg ccttcgagct
     4621 cgtgaatcgc gagctcgcga gcacgctcga cgccgcgcgc agccagctcg aggactacgt
     4681 cgacggccgc gcggcggccg acgcgttgtt gcgaacggcc gagcatctgc acacggcgcg
     4741 cggcgcactg aagatcgtcg agattcacgg cgcggcgctg ctcgccgagg aaatggagca
     4801 gacgtgccgg cgccttgcgg aggcaaagga cgacgacaag tcggaacagg gcatcgaggc
     4861 gctgacgcgc gcgatggtgc agctgcccgc gtacctcgag cgcttgctct ccggtggcaa
     4921 agacgtggcg ctggcgctcc tgccgctgct gaacgatctg cgccaagcgc gcgacaagcc
     4981 ctcgttgtcg gagggtacgc tcgtactgct gaactccggc cccttcgagc gcaccgttgc
     5041 gccgcggacc ggcgctgcgt ccgccgacgt cggccgcggc ttcgacaagt tggcgcagcg
     5101 gctgcggccg ccgttccagg cggccttgct cgggtggatc aagggcgcgg agcccgcgcg
     5161 tttcctcgac gagctgctgc gcgtctgcac gaatctcgag cgttcggccg cgagcgagca
     5221 ggtcaagcag ctatggaccg tgatgacggc cgtgctgacg gcgatccgca gcggcggcct
     5281 cgaagccagc gttacgttga agcgtctgat cggccaagtc gaccgccagc tgaaacggct
     5341 gatcgacggc ggcgaatcgg cgctcgtcaa cgcgccgccc gtcgagctgc tcaacagcct
     5401 cctttattac gtcgcgaaag ccaactcgaa ggacccgcgc atcgaggcgt tgcgcaagca
     5461 gtacaacctc agcgagatgc tgcccgggca agcgcaagtc gagcaggccc gcgaagggct
     5521 gtccggcccg agcgtcaagc tcatgcgcac ggtggcgcag gccatcaaag aagacctcgg
     5581 caccgtcaaa gacgcgctcg acatcttcgt ccgcaccggc atgcaaggcg tcgacaagct
     5641 gagcccgcag gtcgacatgc tgaagaagat cggcgacacg ctcggcgtgc tcgggctcga
     5701 caaggcgcgc ggccagattc agcgcgagac gcaggagctc gcggcgatcg ttttgaacgg
     5761 taaggtcctc gatcagagcg ggctcgaaaa gatcgctgcc acgttgctcg acgtcgagga
     5821 cacgctcgat cgcgagctcg tgcgcgccgt gatgcccggg gacgagtcgg aagctcccga
     5881 ggcaaccggc gaggcggaca cgcagcaacg ccaagtgacg caggccgtca tgggcgaatg
     5941 caccgtgaac ctcgcgaaag tcaaagaagc cgtcattcag ctcgtcgatc acccgaccga
     6001 tacgcggccg ctcgagcagg ccaagccgca gctgcgcggc atcacggccg gcttgttgat
     6061 gttgaacaag acgaaggccg tcagcgtcgt cgaacgcgtc gggtccgtca tcgcgacgcg
     6121 gcttgcagcg ggcacgaagc tgcggccgga gtatctcgag cgactcgcgg acgcaatcgt
     6181 cagcgtcgag tactacatgg agaccgtcag cgcgggccgc agcgacccgt ggtacatgct
     6241 cgagaacgcc gaccgctgtc tcgacctgct cgagacgttg cccgtcgcga agcccgcggc
     6301 gcccacggcg gcggggggtg ccgcgccgcc cccgcgtccc gccgccagca agcccgccgc
     6361 ccctccgccc cgcgtcatgc aagtcgagga agggcgctcg gatcccgagc tcgtcgaggt
     6421 cttcatcgag gaagccaagg aagagatcga aaatatccgc cgcaatctgc cggcgtgggc
     6481 ggcggatcgc acgaactccg aggcattgat cgccacgcgc cgctcgttcc acacgttgaa
     6541 gggcagcggc cgcatggtcg gcgcgcagct gatcggcgag ttcgcctgga gcatcgagaa
     6601 tctgctgaat cggctcatca accagacgct cgagccgacg ccgaacatga tcgcgttcat
     6661 ctcgtcggcg agcggcgctc tgccggcgct gctcgagcaa ctcgagatcg ggttgccgcc
     6721 gaagatcgac gtgcagctgc tgatgaagca ggccgaagcg ttcgccgacg gcgatccgga
     6781 ggccgcaagc ttgacgagcc agtcgttgcg gacgccggcg ttgcctgcgg cggccgaggc
     6841 cgcggcgcct gcgacgtcag agcccggcat ggatcccgtg ctcgtcgaca tcttcgtgaa
     6901 ggagatgcgc ggccatctgg aggtgattcg gcgcttcctc gccggagcca cacctggtgg
     6961 tgggccgcat acggtcgagg agccgctgtt ccgcgcatgc cacacgctgc tcggaagtgc
     7021 gcgcatggcc ggcttcgagc cggcgatgca ggtcgcggcg ccggtcgcgg agcacatgcg
     7081 caagtatttc gactccggca tcggcgtgcc ggattcaggc gtcgcggcgt tgcgcgtcgc
     7141 ggccgatcag gtcgacgcga tggccaatgc gctggcggcg ggccgcacgg tcgtagtcga
     7201 tcccgccacg ctgaaggcgc tcgacgggct gcagtttcgc gagcctgcat ccgcgtcccc
     7261 cgcggcgccg ctgccgtctg cgtcggcgcc gcaagtgccg ccgccggtcg agcaagccgc
     7321 ggccgcgccg cccgtcgagc gggaagccga gccggaagcg gcggcgagcc ggttcgaccc
     7381 cgagatcgcc gcgatcttcg ccgaggaagc ggccgagatc ctcgacaact cgcaagccgc
     7441 gctgcaggaa gttcgccagc gccaagatca ggcggccgtc gcgatgctgc agcgctatct
     7501 ccacacgctg aagggcggcg ctcgcatggc gggtgtgctg ccgatgggcg acttgagtca
     7561 tgcgctcgag acgctgctgt cgcgcatcgc cgaggggcga agccgcccga cgccggcggc
     7621 gctcgagctc gtgcaacgcg gtttggatga gctgcagcag atgcgcgacg ccatcgacgc
     7681 gggccgcgcg gttgccgcgg cgagcgagct cgtcgcacgg ctcgaagcgt tcgaggcacc
     7741 ggtcgtcgcg gagagcgtgc cgccggcgcc gagcacgtcg acccccgtgc aaccggccgt
     7801 cgctgcggtt gccgagatag tcgctcacga accgccggcg gcgacggcac ccggcgcgcc
     7861 gcccgagacg gtgccgatag ccgagctcgc gggcgccgat ttcgagcagc tcgagtcgat
     7921 cgagctgaca ccggcgagcg tgccgccggc cgatgcccga gccgccgcgg aggtcatcga
     7981 agagccgacc gtcgatcagc cggtttcctt cgaagagccg gtggcggagc cgacgatcgt
     8041 cgagccgaag gtcgagcttg ccgtccctcc ggctgccgtg gcgcagcctg tcgaacccgc
     8101 tccggttgcg gttgctcctg ctcctgcgca ggtcgagcag cgcagcgccg aacgagccga
     8161 gaccgcgcgc gtggatgcgg gcttgctcga tgcactattg aacggcgccg gcgagatcaa
     8221 catcttccaa tcgcggctga gtcagcagct gcactcgatc gagttccatc tcggtgagct
     8281 cggcggcacg gtctcgcggc tgcgcgagca gctgcgcaag ctcgaggccg agaccgaggc
     8341 gcaaatcctg catcgtcacc aagacgacgc cgagattgag agcgggttcg atccgctcga
     8401 gctcgaccgc tattcgacga ttcagcaatt gtcgcgcgcg cttgcggagt ccgcgaatga
     8461 cgtcgcgagc atcaacgagc tattgcacgg cctgacgaac gaggccgaca ccttgctgac
     8521 gcagcaagct cgagtgacgt ccgacctcca gagcggcctc atgcagacgc gcatggtgcc
     8581 gttccagcgg cacgtgccgc gcttgcaacg aatcgtgcgg caagcttgcg ccgacaccgg
     8641 caaacaagcc gagctcgtcg tcgacggcga aggcaacgag atcgaccgcc aggtgctcga
     8701 gtcgatgctg ccgccgttcg agcacctgtt gcgcaacgcg atcgcgcacg gcatcgagaa
     8761 gccggccgtc cggcagcagc gcggcaagcc ggaaatcggc caggtgctgc tgaagattcg
     8821 ccgcgaaggc tccgaggtca tcatcgaggt cggcgacgac ggcggtggcc tcgatctcgc
     8881 cgcgattcgc cgcaaaggct atgaaaaagg cctcctcgcc gagaaccagc gcatcacgga
     8941 cgagcaggcc atggagctga tcctgcagcc gggcttcagc acggccagcg agctcacgca
     9001 agcggctggg cgcggcgtcg gcatggacgt cgtcgacaac gagatcaaga agcttggcgg
     9061 gtcgatgcgc atcgagacgc gcaccggcga gggcacgcgc ttcgtgatcc gcttgccgta
     9121 cacgctcgcg atcacgcacg cgctgatcgt caacgtcggc gaggaaacat tcgcgttgcc
     9181 gttgccgacc gtcgaaggca tcacgcggct gtcgcgcgac aaaattttga agcacctgac
     9241 cgaggacgag ccgaagctcg actacggcgg catcgtctac cgcatccagc acctcggcag
     9301 cctcgtcggt gccgcaccgt cggcgttgcc ggaggacgag aacgccgtgt cgctcgtgct
     9361 gattcgcgcc ggcgacaact ccacggcgct cttgatggac tcgctcgaag gcagccgcga
     9421 gatcgtcgtc aagacgctgg gcccgcacat cgcgagcgtt cccggagtca cgggggcgac
     9481 cattctcggc gacggccgcg tcatcatgat tctcgatccg gggacgctcg tgcgctcgca
     9541 gcggccagcc gacgtagtcc gcgcagcgcc gaagcccact gcgtcgactc agctgacggc
     9601 gctcgtcgtc gacgactcga tcacgatgcg gcgcgtgacg cagcggctgc tcgaacggcg
     9661 tggtgcgaaa gtccacacgg cgcgcgacgg tctcgacgcg atcacggtgc tgcaagagca
     9721 caccgtcgac atcattctgc tcgacatcga gatgccgcgc atggacggct atcagttcgc
     9781 gacgcacgtc cgcaacgatc cgaaattaaa gcagctgccg atcatcatga tcacgtcgcg
     9841 ctccggcgag aaacaccgcg cgaaggcgat cgagatcggc gtcaacgatt atttgagcaa
     9901 gccctaccaa gagagccagc tcaccgctgc cattggggcg ttactcggca gggagctctg
     9961 aggccatgga gcagaaagag ctgtacagct tgctgatacc gctgcagaac gagcggctgc
    10021 tcgtgccgcg catgtgtgtt gcggaggtca tcgcgttcgc cgacacggcg cgcagccgcg
    10081 acgatgcggc ccccgattgg tacctcgggg ccatcgagtg gaacaaccgg cgcgtgcccg
    10141 tggtctcgtt cgatgcgccg aaaggcgacg agcgcaaatc gaagcgcagc cgcacgcgcg
    10201 tcgtggtgtt ccacgcaatc acgcaggagc tcaagggcgg gtattacggc atcctgacgc
    10261 aaggcttccc gcagctcgtg cgcgtcaatc ccgatgtcct gggtctcgag accgagcagc
    10321 cgttgccgga gttctttcct gccatctgcc gtgcccggat gatccacgaa tatccgttgg
    10381 tccccgacgt cgagcggctc gaaaaaatga tcgcggagct gccgctgacg gcgtgacggg
    10441 tttcgggcta gctcaaagat ctccccaacg cgactgagcg atggcgcaaa cggccagcgc
    10501 cgcggtttcc gctcgcaaca cgcgtcgtcc caacgtcatg cgggcgaact ccgccgcatc
    10561 gagccggcgc caatccgccg gaccgaaacc gccttcgggc ccgacggcga cgatcagggc
    10621 cggctccggc agcgcctggc tgccgaacgc gtgctcggcc ttggggtcca gagcgacgcg
    10681 cgcgacaccg gccggcagtg cggcgaacgc gtcgaccaac gtcgccggcg gctcgatgct
    10741 cggcggccga tggcggccgc attgctcgca agcgctctgc gcgatcttgc gccaatggtc
    10801 gacgcgccgt gccgtgcgtt cctcgtcgag cttcacgacg ctgaactcgg tatagacggg
    10861 caccaaagct cgcacgccga gctccgtggc tttctgaacg atcagatcca tcgcgtcgga
    10921 cttcggcaac gcttgcatga
//
LOCUS       DATLEE010000100         7021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10489521, whole genome shotgun sequence.
ACCESSION   DATLEE010000100 DATLEE010000000
VERSION     DATLEE010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10489521"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2215)
                     /locus_tag="VM692_02205"
     CDS             complement(<1..2215)
                     /locus_tag="VM692_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUQ51004.1"
                     /translation="MTGKEDHSTERARDERPLTDDERFRSLADTAPAMLWVTQPDGSC
                     SFLSRDWYEFTGQTESAALGTGWLDAVHPDDRERSDATFAAANDKREAFALEYRLRRH
                     DGNYRWVLDSGRPRFDGERLLGYVGSVIDITERKRSELLDNEQKRVLELIAAGRPIEE
                     CLRSIDVAANRLVPGILACGVIAAADAVRRSDELAVYYPHAVGEALRDATDATCHSTP
                     VFGRAGRAIASFRLWLPEPRPPSDWELRVADFAAHAAGIALERERAAAALQDSDTRFR
                     IAADAAESVVYDIDVTSGDEAVAEVHGLEHIIGEHAHERLTASWWESRIHLADLPAHR
                     ALVDASVADPECSSYRSEYRVQRIDGSWRSVEDRARIRRRSDGTATRIVGTIHDITER
                     KQAERDVRRSREQLELLSDTVPALISYIGVDRRYRTCNAEYSKWFGLANEQIVGRPMS
                     EVIGPDAWRVVGPHLERAFAGEASEYEAEVNYRHGGKRWIHARYTPHRDASGAVIGVV
                     CLVNDITSRRQTTHARARLAAIVDSSEDAIVSKNLDGIITSWNNGAVRLFGYTAEEAV
                     GKPVMMLIPEERRYEEEKILARIRHGEGIALYETVRQRKDGSRLDVSLSVSPIVDEGG
                     QIVGASKIARDITARKRAEEDVRRSREALNGLVDRAPFGIYIVNSELQIVQMNARSQT
                     GAFYNVRPVIGRDLVQVMRILWPEAVATEMVAAFRNTLETGESYRSRNFTSPRADT"
     gene            2348..3046
                     /locus_tag="VM692_02210"
     CDS             2348..3046
                     /locus_tag="VM692_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HUQ51005.1"
                     /translation="MLLIRRSGDRGRAKLGWLDSHHTFSFADYHDPRFMGFGPLRVIN
                     EDRVQPGQGFGTHGHQDMEIISYVLEGELAHRDSMGNGSVIRRGDVQRMSAGTGVQHS
                     EFNHSKTEPVHFFQIWLFPDRRGLTPGYEEKHFDDDAKRGRLKLVASQDGRDGSVVVH
                     QRADLYSALLAAGDEVTHSTERTAKGWIQVASGSVTVNGEKLEAGDGAAIAYDETVAI
                     KSNADSELLLFDMR"
     gene            3112..3771
                     /locus_tag="VM692_02215"
     CDS             3112..3771
                     /locus_tag="VM692_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51006.1"
                     /translation="MSKGAAPPMTQPPVAPTGTAAAQPVPQQQRHGRTAASMAVQVVL
                     IALGVFLGLAGEEWRGDRENHRLAIETLQRFRTEVTANREAVAQVKDYHSERHAELIA
                     YFAATEEQKSGVALHIAGIQPPRFERTAWDLALATGTLTYIDSELAFALSRTYAVQAT
                     TDQLGRGLTDAMYLRSPDGDPEGFFSALKLYYDDLTEIEPNLLPVYDKLLTAIDEALA
                     K"
     gene            complement(3815..5272)
                     /locus_tag="VM692_02220"
     CDS             complement(3815..5272)
                     /locus_tag="VM692_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUQ51007.1"
                     /translation="MSGFEGKNFIGGEWRPASSGRRFEQRNPAFLHEVTGTFADSAAA
                     DIDAAVVAAQAAFPAWRALSPLKRKAHLDKALRAMSARRDEIAAAITRENGKALREAR
                     GEVDSAIKEMDYQIAEGVRLCGQTVPVEVDGTLAYSTREALGPVALVTPWNFPFNVPC
                     RKATPALMGGNTVVLKPASLTPRTALLFTELLADGGLPPGVVNCVTGGGGTVGAALVG
                     DTRIKAISFTGSTPVGKSIHLAAARNLTPTQLEMGGKNPAIVLPDADLDLAVAEIVKG
                     AFACSGQWCTSTSRVIVLEEIAAELTQRLVAAASALVVRDGATEDCGMGPVCGAKQKA
                     DILEHIAKGRAEGARLLTGGSALAGEDYDHGCFIAPTVFDRVTRGMSVARDEIFGPVL
                     VIMVVRSFEEAVETANDVAYGLSSSIFTNDLKRALTYMERAEVGLAHVNLMSALKEPQ
                     LSFGGVKESAAGLPEAGSSGIEFFTHHKVCYVKYR"
     gene            complement(5323..6054)
                     /locus_tag="VM692_02225"
     CDS             complement(5323..6054)
                     /locus_tag="VM692_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51008.1"
                     /translation="MLRTLNRWAMIASALTLLAAFWNRNDLPGAIDFRPQLAHEPRQQ
                     PITQAPLIVPYAGVDYRVEPLFNYELYGLVVSYRQHDGTSFMHRNSNDHLNMADVCVV
                     WNDTAFSPTLHKLDFWNGIFTCNVETRDSVAWANFKMNQLSNNHLVSADPYIRHEVAK
                     VRVGDQIRIKGTLVRYGAVGGGLRGTSTTRDDTGDGACETILVAEFSIVAPGFSRWRA
                     AMWAALVVLVATLAVHFTLPYRPYG"
     gene            complement(6158..6835)
                     /locus_tag="VM692_02230"
     CDS             complement(6158..6835)
                     /locus_tag="VM692_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51009.1"
                     /translation="MKTLKLALIPLALAALAGSAGAQQQQQPAANQPPPQPMSFFVTS
                     VGKGDGANLGGLAGADAHCQSLAAAAGRGNATWRAYMSAGAQGAQGVVNARDRIGRGP
                     WFNAKGVLIASNVDDLHQDRNNIRKPTALNEKGEEVNGVGNQPNTHDMLTGSDSAGRL
                     VPGNVALTTCNNWTSNGMGNAMLGHHDRLGGPNASWNSVHSSRSCSQEDLVATGGAGL
                     FYCFVAN"
BASE COUNT         1176 a         2342 c         2315 g         1188 t
ORIGIN      
        1 cggtgtcggc gcgcgggctc gtgaagttgc gcgaccgata ggactcgccc gtctcgagcg
       61 tattgcggaa cgccgcgacc atttcggtcg cgacggcttc gggccagagg atgcgcatga
      121 cctgcacgag gtcgcggccg atcacgggcc gcacgttgta gaacgcaccc gtctgcgacc
      181 gcgcgttcat ctgcacgatc tgcagctccg aattcacgat gtagatgccg aacggcgcgc
      241 ggtcgacgag accgttcagc gcttcgcggc tgcgccgcac gtcttcctcg gcgcgcttac
      301 gcgccgtaat gtcgcgcgcg atcttcgacg ctccgacgat ctggccacct tcgtcgacga
      361 tcggcgagac ggacaacgag acgtcgagcc gcgagccgtc cttgcgttgg cgcaccgttt
      421 catagagggc gataccctcg ccgtggcgga tacgcgccaa tattttctct tcctcgtaac
      481 gccgctcttc gggaatcagc atcatcaccg gcttgccgac ggcctcctcg gccgtatagc
      541 caaacagacg caccgcgccg ttgttccagc tcgtgatgat gccgtctaga tttttgctga
      601 cgatcgcgtc ttcggaggaa tcgacgatcg cggccaatcg cgcgcgcgcg tgcgtggttt
      661 gccggcgcga cgtgatgtcg ttgacgaggc aaaccacgcc aatgacggcg ccgctggcat
      721 cgcggtgtgg cgtgtagcgg gcgtgaatcc agcgcttacc gccgtgccgg tagttgacct
      781 cggcctcgta ctcggaagct tcgcccgcga acgcacgctc gagatgcggc ccgacgacgc
      841 gccaagcatc gggcccgatc acctcgctca tcggccgccc gacgatctgc tcgttcgcca
      901 atccgaacca cttcgagtac tcggcgttgc aggtccgata gcggcgatcg acgccgatgt
      961 aggagatcag cgccggaacc gtgtcgctga gcagctcgag ctgctctcga ctgcgccgaa
     1021 cgtcgcgttc ggcctgcttg cgctccgtga tgtcgtgaat cgtgccgacg atgcgcgttg
     1081 ccgtgccgtc gctgcggcga cggatgcgcg cgcgatcttc gacgctgcgc catgagccgt
     1141 cgatacgctg cacgcgatac tcgctacggt acgacgagca ttcagggtcc gcgacggacg
     1201 cgtcgaccag agcccggtgc gccggcaaat cggcgagatg gatgcgcgac tcccaccaag
     1261 aggccgtgag ccgctcgtgc gcgtgctcgc cgatgatgtg ctcgagaccg tgcacctcgg
     1321 cgacggcttc gtcgccggac gtgacgtcga tgtcgtagac cacggattcc gcggcgtcgg
     1381 ccgcgattcg aaagcgcgta tcgctgtctt gcagcgcggc ggccgcgcgc tcgcgctcga
     1441 gggcaatgcc ggccgcgtga gccgcgaagt ccgcgacgcg cagctcccaa tcgctcgggg
     1501 gccgtggctc gggcaaccac aggcggaacg acgcgatcgc gcgacccgca cggccaaaca
     1561 ccggcgtcga atgacaggtg gcgtcggtag catcgcgcag cgcctcgccg accgcgtgcg
     1621 ggtagtaaac ggcaagctcg tcgctccgcc gcacggcatc ggccgcggcg atgactccgc
     1681 aggccaagat gcccggcacg aggcggttag cggcgacgtc tatcgagcgc aggcattcct
     1741 cgatcgggcg gccggccgca atcagctcga gcacgcgctt ctgctcgttg tccagcaact
     1801 ccgagcgctt gcgctcggta atgtcgatca cggagcccac ataaccgagg aggcgctcgc
     1861 cgtcgaatcg gggccgaccg gagtccaaga cccagcggta gttgccgtcg tggcggcgca
     1921 accgatattc gagcgcgaag gcctcgcgtt tgtcgttcgc ggccgcgaat gtcgcatccg
     1981 agcgttcgcg gtcgtcggga tgcacggcat cgagccagcc ggtgccgagc gcggcgctct
     2041 ccgtctggcc cgtgaactcg taccagtcgc gggacaggaa agaacaggag ccgtccggct
     2101 gcgtgaccca cagcatcgcc ggcgccgtgt ccgccaggct gcggaagcgc tcgtcgtcgg
     2161 tcagcggccg ctcgtcgcgg gcgcgctcgg tgctgtggtc ctccttgccg gtcactggct
     2221 cgccttgggt tctcctgtcg gggaaggtaa cgaggcggag gattcccctg ccgtctcggc
     2281 cgtccggtat ctcgtcgaaa ttgggaggca cgcagtatac cgtaggccaa actgcgataa
     2341 tgagctcatg ttactgattc gacgttccgg cgaccgaggc cgcgccaagc tcggctggct
     2401 cgacagccac cacacatttt cgttcgccga ctaccacgac ccgcgcttca tgggcttcgg
     2461 gccgctgcgt gtgatcaacg aggaccgggt ccagcctggt caaggcttcg gcacgcacgg
     2521 gcaccaagac atggaaatca tttcctatgt cctcgaaggc gagctcgcgc atcgcgacag
     2581 catgggcaac ggctcggtga tccgccgcgg cgacgtccaa cgcatgtcgg ccggcacggg
     2641 agtccagcac agcgagttca atcattcaaa gaccgagccc gtgcacttct ttcaaatctg
     2701 gctgttcccg gaccggcgcg ggctaacgcc gggctacgag gaaaagcact tcgacgacga
     2761 cgcgaagcgc ggccgcttga agctcgtcgc atcgcaggac gggcgcgacg gctccgtcgt
     2821 cgtgcatcag cgcgccgatc tgtactcggc cctgttggcg gccggcgacg aggtcacgca
     2881 tagcaccgag cgcacggcga aaggttggat ccaggtcgcg agcggctcag tgaccgtgaa
     2941 cggcgaaaag ctcgaggccg gcgacggcgc tgcgatcgcg tacgacgaga ccgtcgccat
     3001 caagagcaac gccgacagcg agctcctgct gttcgacatg cgctgacgga gcgggtcttc
     3061 agtcgacctg ccttgacagc tcgcggcgtt tatcgaaacc atgccgcacc gatgagcaag
     3121 ggagccgcac cgccgatgac gcagcccccc gtcgcgccga ccggtaccgc tgcggcgcag
     3181 cctgttcccc aacagcaacg acatggacgt acggcggctt cgatggccgt gcaggtcgtc
     3241 ctcatcgcgc tcggcgtgtt cctcggcctc gcaggtgagg agtggcgagg cgatcgcgag
     3301 aaccaccgcc ttgcgatcga aaccttgcag cggtttcgca cggaggtcac ggccaatcgc
     3361 gaagcggttg cgcaggtcaa ggactatcac agcgagcggc acgcagagtt gattgcttat
     3421 ttcgccgcaa ccgaagaaca aaagagtggc gtcgcgctgc atattgcggg catacagcca
     3481 ccgcgcttcg aaaggacggc gtgggacctg gcattggcaa ccggcacgct cacgtatatc
     3541 gattccgagc tggccttcgc tctgtcgcga acgtacgcag ttcaagcaac cacggaccaa
     3601 ctgggtcgcg gcttgacgga cgccatgtat ctgcgctcgc ccgacgggga ccccgagggg
     3661 ttcttcagcg ccctaaagct gtattacgac gatctcaccg agatcgaacc gaatctcctc
     3721 cccgtgtacg acaagctgct gacggcgatc gacgaagcgc tcgcgaaata gcggcggcgc
     3781 tcggtcgacg cgtcactcat tcgctaccgc gaagtcagcg gtatttcacg tagcagactt
     3841 tgtggtgcgt gaagaactcg atgcccgacg agcccgcctc ggggagcccc gccgcggatt
     3901 ctttcacgcc gccaaatgag agctgcggct ccttgagcgc cgacatgagg ttcacgtgcg
     3961 cgagtccgac ctcggcgcgc tccatgtacg tcagcgcgcg cttcaggtcg ttcgtgaaga
     4021 tcgacgacga caagccgtag gcgacgtcgt tcgcggtctc gacggcttcc tcgaagctgc
     4081 gcacgaccat gatcacgagc acggggccga agatctcgtc gcgcgccacg ctcatgccgc
     4141 gcgtcacgcg atcgaacacc gtcggcgcga tgaagcagcc gtggtcgtag tcctcgcccg
     4201 cgagcgcgct gccgcccgtc aagagccgcg caccttcggc gcggcccttc gcgatatgct
     4261 cgaggatgtc ggccttctgc ttcgcgccgc agacggggcc catcccgcag tcctcggtcg
     4321 cgccatcgcg gacgacgagc gcgctcgccg cggcgacgag ccgctgcgtg agctcggccg
     4381 cgatctcctc gagcacgatg acgcggctcg tcgacgtgca ccactgcccc gagcacgcga
     4441 acgcgccttt gacgatctcg gccacggcaa ggtcgagatc ggcgtcgggc agcacgatcg
     4501 cgggattctt gccgcccatc tcgagctgcg tcggagtcag attgcgggcc gccgcgagat
     4561 ggatcgactt gccgacgggc gtcgagcccg tgaacgagat cgctttgatg cgagtgtcgc
     4621 cgacgagcgc cgcgccgacg gtgccgccgc cgcccgtgac gcagttgacg acaccgggcg
     4681 gcaagccgcc gtccgcgagc aactccgtga acagcaaagc cgtgcgcggc gtcaggctcg
     4741 cgggcttcag cacgacggtg ttgccgccca tcagcgccgg agtggccttg cggcacggca
     4801 cgttgaacgg aaagttccac ggcgtcacga gcgcgacggg gcccagcgct tcgcgcgtcg
     4861 agtacgcgag cgtgccgtcg acctcgacgg gcacggtctg accgcagaga cgcacgccct
     4921 cggcgatctg gtagtccatt tccttgatcg ccgagtcgac ttcgccgcgc gcctcgcgca
     4981 gcgctttgcc gttctcgcgc gtgatcgccg ccgcgatctc gtcgcggcgc gcgctcatcg
     5041 ctcgcaatgc cttgtcgaga tgcgccttgc gcttcagcgg cgacagtgcg cgccaggctg
     5101 ggaaggcggc ttgcgccgcc acgacggcgg cgtcgatgtc ggccgcggcg gagtcggcga
     5161 aagtgcccgt gacctcatga aggaaggcgg gattgcgctg ctcgaagcgc cggccgctcg
     5221 acgcgggccg ccactcgccg ccgatgaagt tcttgccttc gaagccgctc attgaacggc
     5281 attgtgcgtg gcttcggtca ccgcggctag gcgggcggcc tttcacccgt acgggcgata
     5341 cggcagcgtg aagtgcaccg cgagcgtcgc aacgagcacg accaacgctg cccacatcgc
     5401 ggcgcgccaa cggctgaacc ccggcgcgac gatcgagaat tcagcgacga ggatcgtctc
     5461 gcacgcgccg tcgcccgtgt cgtcacgcgt cgtgctcgtg ccgcgcaagc cgccgccgac
     5521 ggcgccgtag cgcacgagcg tccccttgat gcgaatctgg tcgccgacgc gaactttcgc
     5581 gacctcgtgg cgaatgtacg gatcggcgga gaccaagtga ttgttcgaga gctggttcat
     5641 cttgaagttc gcccacgcga cgctgtcgcg agtctcgacg ttgcaggtga agatgccgtt
     5701 ccagaagtcg agcttatgca gcgtagggct gaacgccgtg tcgttccaga cgacgcagac
     5761 gtcggccatg ttcaggtgat cgttggagtt gcgatgcata aagctcgtac cgtcgtgctg
     5821 acgatacgag acgacgagac cgtagagctc gtagttgaac agcggctcga cgcgatagtc
     5881 gacgccggcg tacggcacga tcaacggcgc ttgcgtgatc ggctgctgcc gcggctcgtg
     5941 cgcgagctgc ggccggaagt cgatggcgcc gggaaggtcg ttgcgattcc agaatgccgc
     6001 gagcagcgtc aatgcgctcg cgatcatcgc ccatcgattc agcgtccgca gcatcggcgc
     6061 acgctagcct gaagctcgcg cctgcggcga cgagcggtgt cacgaaacga ggaagaccga
     6121 agtccggcgt ttcaggcgcc ggacttcggt tgcgaggtca gttcgctacg aagcaataga
     6181 agagacctgc gccgccggtg gcgacgagat cttcttggct gcagctgcgc gagctgtgga
     6241 ccgagttcca cgacgcattt ggtccgccga gccgatcgtg gtggcccagc atcgcgttgc
     6301 ccatgccgtt gctcgtccag ttattgcacg tcgtcagcgc gacattgccc ggcacgagac
     6361 gacccgccga atcggagccc gtcaacatgt cgtgcgtgtt cggctgattg ccgacgccgt
     6421 tgacctcttc gcccttctcg ttgagcgccg tcggcttgcg gatgttgttg cggtcctgat
     6481 gcaagtcgtc gacgttcgaa gcgatcagca cgcctttcgc gttgaaccac ggtccgcggc
     6541 cgattcggtc gcgcgcgttc acgacgccct gcgcaccttg cgcgcccgcg ctcatgtacg
     6601 cgcgccacgt cgcgttgccg cggccggcgg ccgccgccag cgactgacag tgcgcgtccg
     6661 cgccggcgag cccgccgagg ttggcgccgt cgcctttgcc gacgctcgtc acgaaaaagc
     6721 tcatcggttg cggcggcggt tgattcgcgg ccggctgttg ctgctgctgc gcgcctgccg
     6781 aaccggccag cgccgccaaa gcgagcggaa tcagagcgag cttcaaagtt ttcatcggtt
     6841 cccccaaggt tgttgttcga gtgcgcggcc caagtccgcc gcctcgcagg attctaagcc
     6901 tgcctcagcg ccggccgtct gaaggaagct gaagcgagag agaagactgc gcgacgctag
     6961 ccgggatcga cgccggcacg ctggcagaag tactcccagc ttctatcgag cgccagcggc
     7021 g
//
LOCUS       DATLEE010000101         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8253665, whole genome shotgun sequence.
ACCESSION   DATLEE010000101 DATLEE010000000
VERSION     DATLEE010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8253665"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VM692_02235"
     CDS             <1..382
                     /locus_tag="VM692_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HUQ51010.1"
                     /translation="IENLEVEPAAAAAKVIVNSRTGTVVIGANVRVTPAAVAHGSLVV
                     RITETALVSQPNAFGRGDTVVVGQSDIDVDQGDNRMFLFEPGTSLNDIVEAVNQVGAA
                     PGDLVAILEALREAGALRAQLIVI"
     gene            397..1407
                     /gene="flgJ"
                     /locus_tag="VM692_02240"
     CDS             397..1407
                     /gene="flgJ"
                     /locus_tag="VM692_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HUQ51011.1"
                     /translation="MANVALPTNAVDLEGLDSLRRRAHRDDPSAVSDVAVQFEALFIG
                     MMLESARSATLSGGLFDGPETEQYLSLMDRQVALELARQGGFGFGKMLEEQLKPHDAT
                     APAQALALTNSRPPRAAELGRAPQSPVLDAAARPLAPTAPVGRHAGEFPRGLPSSLAT
                     LGGVEPPAPPVEPTPAQFVTSLLPHANAAATALGIEPRLLLAQAALETGWGRAVPQRG
                     AESAHNLFGIKAGPSWDGAAVERLTLEHEGGVTEPQLGRFKAYGSPAESFADYADLIG
                     TARRYAAALENAGEPDAYARALTKAGYATDPHYADKWLSIYHGDRLENAMRGIDLPPQ
                     AP"
     gene            1424..>2107
                     /gene="flgK"
                     /locus_tag="VM692_02245"
     CDS             1424..>2107
                     /gene="flgK"
                     /locus_tag="VM692_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HUQ51012.1"
                     /translation="MADLLNIGLSALLAQQRALTTTSNNIANASTPGYSRQRVELAER
                     QAERLGTGFVGTGVNIAATRRLSDEILADQLRVASGSFSRADAFVSLAESLDDMLAGT
                     DTGLAATLQSFGNTLQDVANDPSSTASRQALLSEARNLVARFHAMDQRMTELSGEVRT
                     RMTAAADRITSLGAELAEVNRQLIASGTAAGRPAPPDLLDQRDRLLEELSKLVQVDAT
                     QQRDGTTSVF"
BASE COUNT          346 a          743 c          704 g          314 t
ORIGIN      
        1 catcgagaac ctcgaggtcg agccggccgc ggccgcagcg aaggtcatcg tcaactcgcg
       61 cacgggcacg gtcgtcatcg gtgcgaacgt gcgcgtgacg ccggccgccg tagcgcacgg
      121 ctcactcgtc gtccgtatca cggaaacggc gctcgtgagt cagccgaacg cctttggccg
      181 cggcgacacc gtcgtcgtcg gccaatccga catcgacgtc gatcagggcg acaaccgcat
      241 gttcttgttc gagcccggca cgagcttgaa cgacatcgtc gaggccgtga atcaagtcgg
      301 cgccgcgccg ggcgacctcg tcgcgatcct cgaggccttg cgcgaagcgg gcgcgttgcg
      361 cgctcagctc atcgtcatct aagcggatcg caagcaatgg ccaacgtcgc gctaccgacg
      421 aacgctgtcg acctcgaggg gctcgactcg ttgcgtcgcc gcgcgcaccg cgacgacccg
      481 agcgccgtct cggacgtcgc cgttcagttc gaggcgttgt tcatcggcat gatgctcgag
      541 agcgcgcgta gcgcaacctt gagcggcggt ctcttcgacg gccccgagac ggagcagtac
      601 ctttcgctga tggaccgcca agtcgcgctc gagctcgcgc gccagggcgg tttcggcttc
      661 ggcaagatgc tcgaggagca gctgaagccg cacgacgcga ccgctccggc acaggctctc
      721 gccctgacga attcgcgccc accgcgtgca gccgagctcg gccgcgcgcc gcaatcgccg
      781 gtcctcgacg cggccgcgag gccgctcgca ccgacggcgc ccgtggggcg gcacgccggc
      841 gaattcccgc gcggcttgcc gtcgagcctc gcgacgctcg gcggcgtcga accgcccgcg
      901 ccgcccgtcg agccgacgcc cgcgcaattc gtcacgagct tgctgccgca tgccaatgcc
      961 gccgccacag cgctcggcat cgagccgcgg ctactgcttg cgcaagccgc gctcgagacg
     1021 ggttggggtc gcgcggtgcc gcaacgcggc gccgagtccg cccacaatct gttcggcatc
     1081 aaggccggcc cctcgtggga cggcgcggcc gtcgagcgct tgacgctcga gcacgaaggc
     1141 ggcgtgacgg agccgcagct tgggcgtttc aaagcgtacg gcagccccgc cgaaagtttt
     1201 gccgactacg ccgacttgat cggcacggca cggcgttatg ccgccgcgct cgagaatgcc
     1261 ggcgaaccgg atgcctacgc gcgtgctttg acgaaggcgg gttacgcgac ggatccgcac
     1321 tacgcggaca aatggctctc gatctaccac ggggatcggc tcgagaacgc aatgcgcggc
     1381 atcgacttgc cgccgcaggc accttaacgg atagagagcg ctcatggcgg acttgctgaa
     1441 cataggtctt tccgcgttgc tcgcgcagca gcgtgcgttg actacgacgt ccaacaacat
     1501 cgcgaacgcg agcacgccgg ggtacagccg gcagcgcgtg gagctggcgg aacgccaagc
     1561 cgagcgcctc ggcacgggct tcgtcggcac cggcgtcaat atcgcagcga cgcggcgcct
     1621 gtccgacgag atcctcgccg atcagctgcg tgtggcctcc ggcagcttca gtcgcgcgga
     1681 cgcgttcgtc tcgttggccg aatcgctcga cgacatgctc gccggcacgg ataccggcct
     1741 cgccgcgacg ttgcagtcgt tcggcaatac tctgcaggac gtcgcgaacg atccgtcgtc
     1801 gacggccagc cggcaagcgc tgctcagcga ggctcgcaac ctcgtcgcac gcttccacgc
     1861 gatggaccag cgcatgacgg agctgtccgg cgaagtgcgc acgcgcatga ccgccgcggc
     1921 cgatcgaatc acgtcgctgg gtgccgagct cgccgaggtg aatcggcagc tgatcgcgtc
     1981 gggcacggcg gcagggcggc cggcaccgcc cgatctcctg gaccagcgcg accgattgct
     2041 cgaggaactg tccaagctcg tccaagtcga cgctacgcag caacgcgacg gcacgacgtc
     2101 ggtattc
//
LOCUS       DATLEE010000102         7331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1604661, whole genome shotgun sequence.
ACCESSION   DATLEE010000102 DATLEE010000000
VERSION     DATLEE010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604661"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VM692_02250"
     CDS             complement(<1..516)
                     /locus_tag="VM692_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HUQ51013.1"
                     /translation="MTDSFGARKPLAVKSATYQIYRLDAVKGAVDTLPYTLKILLENL
                     LRFEDGENITAEDITALANWDPTAEPDREISFTPARVVLQDFTGVPAVVDLAAMRDAV
                     VKLGGKAQMINPLSPAELVIDHSVQVDHFGTPDALAKNTAIEFERNQERYAFLRWGQT
                     ALQNFKVVPPNT"
     gene            complement(581..1702)
                     /gene="mnmA"
                     /locus_tag="VM692_02255"
     CDS             complement(581..1702)
                     /gene="mnmA"
                     /locus_tag="VM692_02255"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010209592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HUQ51014.1"
                     /translation="MSGSPLTVVALSGGVDSAVVALMLRDAGHTVQCLHMSNWEDDGY
                     CDNAREFQDARRVCLELELPLHRVNFAREYREQVFADFLAEYRNGRTPNPDVLCNRQI
                     KFGVMRRYAGRLGATRIATGHYARVATVDGVAQLRKGLDPGKDQSYFLHAVAAEDLAD
                     VLMPLGELKKTEVREIARKAGLAVADKKDSTGICFIGERPFTEFLHQYLPDEPGVIRD
                     ETGHERGRHRGLAYYTLGQRHGLSIGGVADRPENAWYVAAKNAARNELVVVQGHEHPL
                     LKSRALVASGLHWIGPAPDAWQRGEPLRCHAKIRYRQPDQACTVVATRQGSIEVRFAE
                     PQRTPTPGQFVVLYDGDRCLGGATIDAAMAESPALRAAV"
     gene            complement(1740..2243)
                     /locus_tag="VM692_02260"
     CDS             complement(1740..2243)
                     /locus_tag="VM692_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HUQ51015.1"
                     /translation="MTRGIDVTVAAIIERDGKFLMVEERAGDKLVLNQPAGHLEQGES
                     LVAAVAREALEETGHRFEPVHVVGFYLWRSEDADTTYLRIAFCGSVAPSADVAAASLD
                     DGIVAVHWLPRAQLLRRSQQLRSPMVLRCLDDYLAGQRYPLDCLTYIERRAGDIDATD
                     IRLRAFT"
     gene            complement(2251..2715)
                     /locus_tag="VM692_02265"
     CDS             complement(2251..2715)
                     /locus_tag="VM692_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51016.1"
                     /translation="MLTNVAEVRAAVIQVTGVANRTLSIMTHDLEPDVYDHDDFLETL
                     KKFILARTFARVRVLILEPARVLLSASRFVAMGRRLNSYIEFRRVTPDLANHPEAFFI
                     ADEQGLVYRARAESWDGMSDTYEPAVARMYLSKFESLWNACEIEPEIRQLQI"
     gene            complement(2855..4225)
                     /gene="purB"
                     /locus_tag="VM692_02270"
     CDS             complement(2855..4225)
                     /gene="purB"
                     /locus_tag="VM692_02270"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HUQ51017.1"
                     /translation="MDATSLLALSPLDGRYARKAAPLREYLSEYALIRYRTLIEVRWL
                     QHLADEPSVAELASLEPPIKDVLNQIVDEFSVDDATRIKALEAKTNHDVKAVEYYLRQ
                     KLEGVAVAAGNLTSFIHFACTSEDINNLSYALMLRDARQRVLLPAMREITTDLRELAH
                     RYADVPMLARTHGQPATPTTLGKEIANFVYRLQRQTELFGAVAIHGKFNGAVGNHNAH
                     VAAYPEADWIAITDRFIASLDLQTTRYTTQIEPHDWIAEYCHALARFNTVLVDLCRDF
                     WSYISLGYFRSLPVAGEVGSSTMPHKVNPIDFENAEGNLGVANELLAFLATKLPVSRW
                     QRDLTDSTVLRNAGVALGHTLIALEAAGKGLRKLDADLATIAADLGDRWEVLAEAVQT
                     VMRRYGLPDAYEQLKDLTRGRAIDAEALREFIGSLPIPPAERQRLLALEPSSYTGLAA
                     ELARRI"
     gene            4320..5705
                     /locus_tag="VM692_02275"
     CDS             4320..5705
                     /locus_tag="VM692_02275"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HUQ51018.1"
                     /translation="MAADFRVERDSMGELQVPASALWGAQTQRAVNNFPISGLRMPPA
                     FIRALALVKWAAARTNRGLGLLPEPIASAIEKSALEIANGMHEDQFPIDVFQTGSGTS
                     SNMNANEVIAHVASRYAGLAVHPNDHVNMSQSSNDSIPSAIHIAAALELTQKLLPALE
                     HLAETIEAKAKEVAGIVKTGRTHLMDAMPVRLDQELGGWAAQVRADVRRLQGVMPRLS
                     GLAQGGTAVGTGINAHPKFGAEFAAVLAKQTGVAFHPAENYFEALSSQDTAVELSGQL
                     KTTAVSIMKIANDLRWMNSGPLAGLSEIHLPDLQPGSSIMPGKVNPVIPEAAAMVAAQ
                     VIGNDATITIGGQSGNFQLNVMLPVVALNLLQSIELLANVSSALADAAIKGFKVNKPK
                     LDDALERNPILVTALNPVIGYEKGAAIAKQAYKEGRAIRDVAAAQTQLKPEQLQQLLD
                     PLELTKGGIKG"
     gene            5689..6333
                     /locus_tag="VM692_02280"
     CDS             5689..6333
                     /locus_tag="VM692_02280"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HUQ51019.1"
                     /translation="MESKVKTLDVIRRRLAAPRAPRDPRARLLGNIEGPVSPTLREIL
                     DRPGRPASVLLALFERPAGPTVLFTERAAHLKDHAGQVSLPGGRLAAGETAAEAALRE
                     AHEEVGLEPSTVDVIGSLDDFLTGTGFTITPVVGIVSNSTFTAIPDPREVAGVFEVPL
                     TVILDRTAMSVGYFERHGSRLLTYELMYVGRRIWGATAAVLRNFQDVILDEDGT"
     gene            6317..7114
                     /gene="mazG"
                     /locus_tag="VM692_02285"
     CDS             6317..7114
                     /gene="mazG"
                     /locus_tag="VM692_02285"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HUQ51020.1"
                     /translation="MKTEPDTTSLAELLRIMARLRDRERGCPWDLEQSFATIAPYTIE
                     EAYEVADAIERNDLPDLKDELGDLLFQVVFHARMAEEAGAFAFADVARAICDKMLRRH
                     PHVFGSLEVGSSAEQTELWERIKREERAGAPPAGILDDVPVGLPALTRATKLGKRAAS
                     VGFDWPDVAGVRAKIDEELAEVDAAMTGGDESEIAAELGDLLFSIANLTRHLELDPET
                     CLRAANARFARRFHVVESEVAASGKPWSSFAAPELDRLWNEAKKRAK"
     gene            7111..>7331
                     /locus_tag="VM692_02290"
     CDS             7111..>7331
                     /locus_tag="VM692_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51021.1"
                     /translation="MRRQVLSALSAAAADRLRAAGFGPIKPSHWRWSRSVPDRIDLLE
                     VLFADAQGPASHARSSASFSVLAGTFLKG"
BASE COUNT         1253 a         2326 c         2512 g         1240 t
ORIGIN      
        1 ggtgttcggc ggcacgacct tgaagttttg cagcgcggtc tggccccagc gcaagaacgc
       61 gtagcgttcc tggttgcgct cgaactcgat ggcggtgttc ttggccagcg cgtccggcgt
      121 gccgaagtga tcgacttgca ccgaatggtc gatcacgagc tcggccggcg acagcgggtt
      181 gatcatctgc gccttgccgc cgagcttgac gacggcgtcg cgcatcgccg ccaggtcgac
      241 gacggccggc acgcccgtga agtcttgcag cacgacgcgg gccggcgtga aggaaatctc
      301 gcggtccggc tccgcggttg ggtcccaatt tgcgagcgcc gtgatgtcct cggccgtgat
      361 gttctcgccg tcctcgaagc gcaagaggtt ctcgagcagg atcttcagcg tgtacggcag
      421 cgtgtcgacg gcgcctttga cggcatcgag gcggtagatc tggtaagtcg cggacttcac
      481 ggcgagcggc ttacgggcgc cgaaactgtc ggtcattgga acctctgaga ctcggtggaa
      541 cgcccgcgat tataagcggg aaaccgcgtg tagcggcgaa ctaaaccgcg gcgcgaagcg
      601 ccggtgactc cgccatcgcc gcatcgatcg tcgcgccgcc gaggcagcgg tcgccgtcat
      661 agagcacgac gaactgaccc ggcgtcggcg tgcgctgcgg ctcggcgaac cgcacctcga
      721 tcgagccctg gcgcgtcgca acgacggtgc aagcttggtc gggctgccga tagcgaatct
      781 tcgcgtggca gcgcagcggc tcgccgcgct gccacgcgtc gggcgccggc cctatccaat
      841 gcagccccga ggcgacgagc gcgcggctct tgagcagcgg atgctcatgc ccttgcacga
      901 cgacgagctc gttgcgcgcg gcgttcttcg ccgcgacgta ccacgcgttc tcggggcggt
      961 ccgcgacgcc gccgatcgac agcccgtgcc gctggccgag cgtgtagtag gcgagcccgc
     1021 gatgccggcc gcgctcgtgt cccgtctcgt cgcggatgac cccgggctcg tcgggtaaat
     1081 actgatgcag gaactcggtg aacggccgct cgccgatgaa gcaaatgccg gtgctgtcct
     1141 tcttgtccgc gacggcgagc ccggccttgc gcgcgatctc gcgcacctcg gtcttcttga
     1201 gctcgccgag tggcatcagc acgtcggcta gatcctcggc cgcgacggcg tgcaggaaat
     1261 agctttgatc cttgccggga tccaggccct tgcgaagctg agccacgccg tcgaccgtgg
     1321 cgacgcgagc gtagtggccc gtcgcgatgc gcgtggcccc gaggcgccct gcgtagcggc
     1381 gcatcacgcc gaacttgatc tgccggttgc agagcacgtc ggggttcggc gtgcggccgt
     1441 tgcggtattc ggcgaggaag tccgcgaaca cttgctcgcg gtactcgcgg gcgaagttca
     1501 cacggtgcag cggcaactcg agctcgaggc acacgcggcg agcatcctgg aactcgcgcg
     1561 cgttgtcgca gtagccgtcg tcctcccaat tgctcatgtg caggcactgc acggtgtgcc
     1621 cggcatcccg caacatgaga gcgacgacgg cggaatcgac gccgcccgac aaggcgacga
     1681 cggtgagagg gcttccggac atgccggtga ttttagcagc cgcccccgag caagcgactt
     1741 taggtaaacg cgcgcaggcg tatgtcggtc gcgtcgatgt cgccggctcg gcgctcgata
     1801 taggtcagac aatcgagcgg atagcgctgg ccggcgagat agtcgtcgag gcagcgcagc
     1861 accatcgggc tgcgcagctg ctggctgcgg cgcaagagct gcgcgcgcgg cagccagtgc
     1921 acggcgacga tgccgtcatc gagcgaggcc gccgcgacgt ccgcggaagg tgcaacggag
     1981 ccgcagaagg cgatacgcaa gtaagtcgtg tcggcgtctt cgctgcgcca gagatagaat
     2041 ccgacgacgt gcaccggctc gaagcggtgg cccgtttcct cgagcgcctc gcgagccacg
     2101 gcggcgacga gcgattcgcc ctgctcgaga tgcccggcgg gctgattcag cacgagcttg
     2161 tcgcccgcgc gctcctcgac catcaagaac ttgccgtcgc gctcgatgat cgcggcgacc
     2221 gtgacgtcga tgccgcgtgt catcggccta ctatatttga agttgccgta tttcgggttc
     2281 gatttcacag gcattccaca gcgattcgaa tttgctcagg tacatgcggg ccacggcggg
     2341 ctcatacgtg tcggacatcc catcccagct ctcggcgcga gcgcgataga cgagcccttg
     2401 ctcatcggcg atgaaaaacg cttcgggatg attcgcgaga tccggcgtca cccgccggaa
     2461 ctcgatgtag ctgttcagcc gccgccccat ggccacgaac cggctggcgg acaagagcac
     2521 gcgcgccggc tcgaggatca gcacgcggac gcgcgcgaag gttcgcgcga ggatgaactt
     2581 cttcagcgtc tccaagaaat cgtcgtgatc gtagacgtcc ggctcgaggt cgtgcgtcat
     2641 gatcgacagc gtgcggttcg cgactccggt cacctggatc acggccgcgc gcacttcggc
     2701 tacgttcgtc agcacctgac ggacgcctcg cgttttgatc tgttcttgcg gctgcgacat
     2761 ggacggagtt atcagttgtg ccgcggagtt ttacaaaaga gcggctggcg ggctgtgccc
     2821 gcggtcacgg ttttgtgacc cgaaggtcag gctactagat gcgccgcgcc aactcggcgg
     2881 cgaggcccgt ataagaggag ggctcgagcg caagtagccg ttgccgttcg gccggcggga
     2941 tcggcaggct gccgatgaat tcgcgcaggg cctcagcgtc gatggcccgg ccacgcgtga
     3001 ggtctttgag ctgctcgtag gcgtcgggca agccgtagcg gcgcatgacg gtctgcacgg
     3061 cttcggccag cacttcccaa cggtccccga gatcggccgc gattgtcgcg aggtccgcgt
     3121 cgagcttgcg gagccctttg ccggcggctt cgagcgcgat caacgtatgc ccaagggcca
     3181 cgccggcgtt gcgcagcacg gtcgagtcgg tcaggtcgcg ctgccagcga gagacgggca
     3241 acttggtcgc gaggaatgcc agcagctcgt tggccacccc tagattgccc tcggcgttct
     3301 cgaaatcgat gggattgacc ttgtgcggca tcgtcgacga gccgacctcg ccggcgacgg
     3361 gcagcgagcg gaaatagccc aagctgatgt agctccagaa atcgcggcag agatcgacga
     3421 gcacggtgtt gaagcgcgcc aaggcgtgac agtactcggc gatccagtcg tgcggctcga
     3481 tctgcgtcgt gtagcgcgtc gtctgcaaat cgagcgaagc gatgaagcgg tccgtgatcg
     3541 cgatccagtc ggcctcggga taggcggcga cgtgcgcgtt gtgattgccg acggcgccgt
     3601 tgaacttgcc gtgaatcgcg acggcgccga acagctccgt ctgccgctgc aagcgataga
     3661 cgaagttggc gatctctttg ccgagcgtcg tcggcgtcgc gggctgcccg tgcgtgcgcg
     3721 cgagcatcgg cacgtcggca tagcgatgcg cgagctcgcg cagatccgtc gtgatctcgc
     3781 gcatcgccgg cagcagcacg cgctggcgcg catcgcgcag catcagagcg tacgaaagat
     3841 tgttgatgtc ctcggacgtg caggcgaagt gtatgaacga cgtcaggttg ccggccgcca
     3901 cggcgacgcc ctcgagcttc tgccgcaagt aatactcgac ggctttgacg tcgtggttcg
     3961 tcttcgcttc gagcgccttg atgcgcgtcg catcatcgac gctgaactcg tcgacgattt
     4021 ggttcagcac gtccttgatc ggcggctcga gcgaggcgag ctcggcgacg ctcggctcgt
     4081 cggcgaggtg ttggagccag cgcacttcga tcagcgtgcg atagcggatc agcgcgtatt
     4141 cggaaaggta ctcgcgcagc ggcgcggctt tacgcgcgta acgcccatcg agcggcgaaa
     4201 gtgcgagaag cgaggtggcg tccaacgtgg ggccgaagcc tatacaagcg ttttcggagt
     4261 gcgcatcata caccgcgagc ccacgctaaa gcgggctcga tcaatcttcg ggagatggca
     4321 tggcggccga ttttcgtgtc gagcgtgaca gcatgggcga gctacaagtg cccgcgtccg
     4381 cgctctgggg cgcgcagacg caacgcgcgg tcaacaattt tccgatcagc ggattgcgca
     4441 tgccgccggc gttcattcgc gcgttggcgc tcgtgaagtg ggccgcggcg cgcacgaatc
     4501 gcggcctagg cctgctccct gagccgatcg caagcgcgat cgagaagagt gcgctcgaga
     4561 tcgcgaacgg catgcacgag gatcaattcc cgatcgacgt gttccagacg ggctcgggca
     4621 cgagctcgaa catgaacgcc aacgaggtga tcgcgcacgt cgccagccgc tatgccggtc
     4681 tcgccgtgca tcccaacgat cacgtcaaca tgagccagag cagcaacgac tcgatcccgt
     4741 cggcgatcca catcgcggcc gcactcgagc tcacgcagaa actgctgccg gcgctcgagc
     4801 atctggctga gacgatcgag gcgaaggcaa aggaagtcgc cggcatcgtc aagacggggc
     4861 gcacgcactt gatggatgcg atgcccgtgc ggctcgatca ggagctcggc ggctgggccg
     4921 cgcaggtccg cgccgacgtt cgccgtctcc agggggtcat gccgcggctc tcgggcctcg
     4981 cgcagggcgg cacggccgtc ggcacgggca tcaacgcgca tccgaagttc ggcgccgagt
     5041 tcgcagcggt gctcgcaaag cagacgggcg tcgctttcca tcccgcggag aattacttcg
     5101 aagcgctgag cagccaagac acggccgtcg agctgtcggg gcaattgaag acgacggccg
     5161 tcagcatcat gaagatcgcg aacgacttgc gctggatgaa cagcgggccg ttggcgggct
     5221 tgtcggaaat tcatctgccg gatttgcagc cgggcagcag catcatgccg ggcaaagtga
     5281 atcccgtgat tcccgaagcg gccgcgatgg tcgcggccca agtcatcggc aacgatgcga
     5341 cgatcacgat cggcggacag tccggcaatt tccagttgaa cgtcatgctg cccgtcgtcg
     5401 cgttgaactt gctgcagagc atcgagctgc tcgcgaatgt gtcgtccgcg ctcgccgacg
     5461 ccgcgatcaa aggcttcaag gtcaacaagc ccaagctcga cgacgcgctg gagcgcaatc
     5521 cgattctcgt gacggcattg aatcccgtca tcggctacga gaaaggcgcg gcgatcgcga
     5581 agcaggcgta caaggaaggc cgtgcgattc gcgacgtagc cgccgcgcaa acgcagctca
     5641 agcccgagca gctccagcag ttgctcgatc cgctggagct cacgaagggt ggaatcaaag
     5701 gttaagacgc tcgacgtcat tcgccgccgg ttggcggcgc cgcgagcgcc gcgcgatcca
     5761 cgcgcccgac tgctcggcaa catcgagggc ccggtttcgc cgacgctgcg cgagatactc
     5821 gatcgccccg ggcggcccgc ctcggtgctg ctcgcgctgt tcgagcgccc cgcggggccg
     5881 acggtgctgt tcacggagcg cgcggcgcat ctgaaggatc acgcggggca agtcagcctg
     5941 ccgggcggcc ggctcgcggc cggcgagacc gcggccgaag ccgcgctgcg cgaggcgcac
     6001 gaggaggtcg gcctcgagcc ctcgacggtc gacgtgatcg gctcgctgga cgacttcctg
     6061 acggggacgg gtttcacgat caccccagtc gtcggtatcg tcagtaactc aacgttcacc
     6121 gcgatacccg atccgcggga ggtcgccggc gtgttcgagg tgccgctgac ggtaatcctc
     6181 gaccgtaccg cgatgagcgt cggctacttc gagcgccacg ggtcgcgcct gctgacgtat
     6241 gagctcatgt acgtcggccg ccgcatttgg ggtgcaaccg ccgctgtgct gcgcaatttt
     6301 caggacgtga tactcgatga agacggaacc tgacaccacg tcgctcgcgg agttgctgcg
     6361 aatcatggcg cggctgcgag atcgcgagcg cggctgcccc tgggacctcg agcagtcgtt
     6421 cgcgacgatt gcgccctaca cgatcgaaga ggcgtacgaa gttgccgacg cgatcgagcg
     6481 caacgacttg ccggatctca aagacgagct cggcgatctg ttgtttcagg tcgtgtttca
     6541 tgcgcgcatg gccgaggagg ccggcgcgtt cgcgttcgcc gatgtcgcga gggcgatttg
     6601 cgacaagatg ctgcgccgcc atccgcatgt gttcgggtcg ctggaggtcg gcagcagcgc
     6661 ggagcagacg gagctctggg aacgcatcaa gcgcgaggag cgcgcgggag cgccgccggc
     6721 cggaattctc gacgacgtgc ctgtcgggct gccggcgctg acgcgcgcaa cgaagctcgg
     6781 caaacgcgcg gcgtcggtcg gcttcgactg gcccgacgtc gcgggcgtgc gcgcgaagat
     6841 cgacgaggag ctcgcggagg tcgacgccgc aatgaccggc ggcgacgaga gcgaaatcgc
     6901 ggcggagcta ggcgatctct tgttctcgat cgcgaacctg acccgtcatc tcgagctcga
     6961 ccccgaaacg tgtctgcgtg cggccaatgc tcgcttcgcg cggcggtttc acgtcgtcga
     7021 atccgaggtg gccgcgagcg gcaagccttg gtcgagcttc gcggcgccgg agctcgatcg
     7081 cctctggaac gaagccaaga agcgcgcgaa atgaggcggc aggttctctc ggcgctgtct
     7141 gcggcggccg ccgaccggct gcgcgcggcc gggttcggtc ctatcaaacc gtcgcactgg
     7201 cgctggagcc gcagcgtgcc cgatcgcata gacctgctcg aggtgctgtt cgccgacgcg
     7261 caaggcccgg cgtcgcacgc taggtcgtcg gcctccttct ccgtgctggc cggaacgttc
     7321 ctcaaaggca t
//
LOCUS       DATLEE010000103         2331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5330610, whole genome shotgun sequence.
ACCESSION   DATLEE010000103 DATLEE010000000
VERSION     DATLEE010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5330610"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VM692_02295"
     CDS             complement(<1..216)
                     /locus_tag="VM692_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HUQ51022.1"
                     /translation="MAQVFKPSSNTYARLSLAVVGILGGLAFGGLYQFNTLRYVTGNH
                     EALAQPVQFSHKHHNGELGIDCRYCHTS"
     gene            782..>2331
                     /locus_tag="VM692_02300"
     CDS             782..>2331
                     /locus_tag="VM692_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HUQ51023.1"
                     /translation="MTKLSSSTSGRRTASLAFLFVTAAACWASSAAAALLEVPADYAT
                     IQAAVTAAGPGDTVEVSSGVYNERVTFSSSGLSGSPITLTAKAGHSPVIDGTGIPTTD
                     LEGLVYIEDESYIVVSGFEIRNFSASAPSHFPAGIWVRGSAHHIEIRDNDVHDIRNAG
                     CASCGAHGIAVYGTKASASIHDVVIDGNEVHDLVLGWSESLVVNGNVEDFEITNNTVH
                     DNNNIGIVAIGFEGECTGCSDALDRARDGVIADNLVYDIDSIDNPSYAGSRSANGIYV
                     DGGTRITIERNTVHDCNIGIELASEHDGKSTSEVIARSNFVSRSHSIGISIGGYDTHR
                     GSTTGCAVVHNTLYDNDTDHSGGGELLLQYDVRDNVVENNIVYANSQNQFVANEFSLT
                     SGNTIDYNLYFSAAGAASSEWRWTTTPYTGFAAWKAGSGNDAHSTFEDPILVSPATGD
                     LHLGAGSPAVGAAIALAPSIAGAFDIDGTPRISGASADKGADELACGNGSMDADELCD
                     DGDIEDGDG"
BASE COUNT          428 a          783 c          739 g          381 t
ORIGIN      
        1 tgaggtgtgg cagtagcggc agtcgatgcc gagctcgccg ttgtggtgct tgtggctgaa
       61 ctgcaccggc tgggcgaggg cctcgtggtt gcccgtcacg taacgaagtg tgttgaactg
      121 gtagagaccg ccgaaggcca ggccgccaag gattccgacg acggccagcg acagtctcgc
      181 gtaagtattg ctgctcggtt tgaaaacctg ggccatggga tccggtatgt caccgaccgt
      241 tggtggcaac gcaaaacgct gccttaatcc gcctgcgaaa gtcttgaagg aagtcttatt
      301 ctcgacgcac cgactcctgt cccgcgacgt cgtcgatgcc cgtgcgcggc ccctcggtcc
      361 gtgcgacgcg cctccataga gagttcatgc gtgagggtcg aacgtttcgt gcacaaaacg
      421 gagcggcgcc ttgttgccgg aaatgcgcgg gaaacgcgcg caggaagcag cgggcgccga
      481 tcgcgggcac cggcccggtt cctccccgga cgggtggccg gcgggacggc tggaacgatc
      541 ggccgaatct gttgacagcc gtgaaagcac acagggctgc aaaaacatgc taaccccacg
      601 gaactacagc gttttccgct gaaattcgcc gttttccttc cttatatacg tccggtacgc
      661 ggccgcccgg cgaatcgggc ccttcgagcc tccgacggcg cccttccgcc gactcctctt
      721 cccgatgccc cgcgcctggg cttatctagg cggcgccgcc cacggcggca cgggggattt
      781 catgaccaaa ctttcttctt cgacctccgg ccgccggacg gcctcgctcg cgttcctctt
      841 cgtgaccgcc gccgcttgct gggcgtcgtc cgcggcggcc gcgctgctgg aggtcccggc
      901 ggactacgcg acgatccagg cggccgtgac cgcggccggc ccgggcgaca cggtcgaggt
      961 gtccagcggc gtctacaacg agcgcgtcac cttctcgtcg agcgggctct cgggaagtcc
     1021 gatcacgctg accgccaagg ccgggcactc gccggtcatc gacggcaccg gtatcccgac
     1081 caccgacctc gagggcctgg tctacatcga ggacgagtcc tacatcgtcg tctcgggctt
     1141 cgagatccgg aacttcagcg catcggcacc gagccacttt ccggccggca tctgggttcg
     1201 cggcagcgcg caccacatcg agattcgcga caacgacgtg cacgacatcc ggaacgcagg
     1261 ctgcgcgagc tgcggtgcgc acggcatcgc cgtctacggc accaaggcgt ctgcttcgat
     1321 ccacgacgtc gtcatcgacg gcaacgaggt acacgatctc gttctcggat ggagcgagtc
     1381 gctggtcgtc aacggcaacg tcgaggactt cgagatcacc aacaacacgg tgcacgacaa
     1441 caacaacatc ggcatcgtcg ccatcgggtt cgaaggcgaa tgcacgggct gcagcgacgc
     1501 gctcgaccgg gcacgcgacg gcgtcatcgc ggacaacctc gtctacgaca tcgactcgat
     1561 cgacaacccg tcctacgccg gcagccgatc ggccaacgga atctacgtcg acggcggcac
     1621 ccgcatcacg atcgagcgca ataccgtcca cgactgcaat atcggcatcg agctggcgag
     1681 tgagcacgac ggcaagtcga cgagcgaagt catcgcgcgc agcaatttcg tttcgcgatc
     1741 gcattcgatc ggtatctcga tcggcggcta cgacacgcac cgcggaagca cgacgggctg
     1801 cgccgtcgtg cacaacaccc tgtacgacaa cgacaccgac cactcgggcg gcggcgagct
     1861 gctgctgcag tacgacgtgc gggacaacgt cgtcgagaac aacatcgtct acgcgaactc
     1921 gcagaatcag ttcgtggcca acgagttctc gctgacgagc ggcaacacga tcgactacaa
     1981 cctgtacttc agcgccgcgg gcgccgcatc gtcggagtgg cgctggacga cgacgccgta
     2041 caccggcttc gccgcgtgga aggccggctc gggcaacgac gcgcattcga cgttcgagga
     2101 tccgatactc gtgagccccg ccaccggcga ccttcatctc ggggcgggct ctccggcggt
     2161 cggtgcggcc atcgcgcttg cgccttcgat cgcaggggct ttcgacatcg acggcacgcc
     2221 gcgaatctcc ggcgcttccg ccgacaaggg cgccgacgag ctcgcctgcg gcaacggcag
     2281 tatggacgcg gacgagcttt gcgacgacgg cgacatcgaa gacggcgacg g
//
LOCUS       DATLEE010000104         4115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3839091, whole genome shotgun sequence.
ACCESSION   DATLEE010000104 DATLEE010000000
VERSION     DATLEE010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3839091"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VM692_02305"
     CDS             complement(<1..434)
                     /locus_tag="VM692_02305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HUQ51024.1"
                     /translation="MRTGRRVPAYHIGTLAAAVGAVLGVSTAAKAQQLEEIIVTAERR
                     ELNLQDTPISVMSFGAEALELRGVDDMFELANIAPNLDIKGARGVGNTAPTFQIRGIS
                     GGGGATGERGVGFYVDNVFMPRTTGPVMRVLDIERIEVLRGP"
     gene            complement(567..1751)
                     /locus_tag="VM692_02310"
     CDS             complement(567..1751)
                     /locus_tag="VM692_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HUQ51025.1"
                     /translation="MSKPDQHFDGDAQRSRRVVIKTMSAAGAAALAPGWLAAQAPVDP
                     PSTVSNPPRDFSPGAQPVTYPDPDLVTVDPAFNALRIGNTPIVRLWTGALWSEGPAWS
                     SQGRYLVWSDIPNDRQLRWLEDDGRVTVFRSPTNNSTGNTFDFQGRQVTCEHLLRRVV
                     RYENDGSVVVLADAYDGKPLNSPNDVVAHPDGSYWFTDPAFGGQLYEGVPDAPGGPSN
                     PQGILRQRVGQRAGVGEAQRELPTNVYRVAPNGRVAIAIAADVLPGPPNGLAFSVDYK
                     KVYVVSGGNLHVGDVADDGRVSGVKQFLDLTVDGVACRTDGVRVDVQGNVWCSSNAGL
                     RVGYSGVTVWNSAGALLGRIRLPEVCANVAFGGPKRNRLFMAASQSLYAVYVNTQGAA
                     PG"
     gene            1820..3811
                     /locus_tag="VM692_02315"
     CDS             1820..3811
                     /locus_tag="VM692_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51026.1"
                     /translation="MPKSLRVVLPCLVAGLMAACSPAPETTTAAKEPAAAPPPTAAAQ
                     AAANAAGPTVNWAYYGGNLASQRYSPLEQINAGNAGQLKIAWRFSTGNYGPRPEARNE
                     TTPLMIDGVLYTQAGTTRNVVAVDAKSGELLWVWRPNDGEQRYARAPRTTSGRGPAYW
                     TDGAGNERLFTVTPGFFLAALDPDTGRPVPGFGENGIVDLMVGVRGPEATDKTSIGNS
                     SPALVIGDVVVVGPAHEVGMRPPSQRNFKGDVRGFDVHTGKLLWTFHTIPEAGEFGYD
                     TWLDGSAERTGNAGVWAAMTADEELGYIYLPTEGALADIWGGERHGNNLFSSSLVCLD
                     AKTGERVWHYQLIHHDIWDWDNPTAPILMDLVVDGRPIKAVAQLTKQAFVYTFDRVTG
                     EPVWPIEERPVPQTDVPGEWTSPTQPFPTKPPPYDRQGVSVDDLIDFTPALRAAAIEG
                     VKKFRIGPIFTPPSLANAADGTSGTLTLPNATGGANWEGGAFDPETNVLYVGSVTNPS
                     IFALEPPPSPTDITYINGGGAQLPWLNGLPLIKPPWGRITAYDMNKGEMLWQLASGPT
                     PKAVAENSALKGLDIPPTGSAARPLTLVTKTLLFTADGWGATPALQARDKATGEIVAE
                     VALPGAVGGRPMTYMLDGKQYVVVSVAGSNGAEIVALTL"
BASE COUNT          669 a         1394 c         1406 g          646 t
ORIGIN      
        1 tgcgggccgc gcagcacttc gatgcgctcg atgtcgagca cgcgcatcac ggggcccgtg
       61 gtgcgcggca tgaagacgtt gtcgacgtag aaaccgacgc cgcgctcgcc cgtcgcgccg
      121 ccgcccccgc tgatgccgcg aatctggaaa gtcggcgccg tgttgccgac gccgcgtgcg
      181 cccttgatgt cgaggttcgg ggcgatgttc gcgagctcga acatgtcgtc gacgccgcgc
      241 agctcgagcg cttcggcgcc gaaactcatg acggagatcg gcgtgtcctg caagttcaat
      301 tcgcgccgct cggcagtgac gatgatctcc tcgagctgtt gcgctttggc cgcagtcgaa
      361 acgccgagca ctgcgccgac ggcagccgcc aatgttccga tgtgatacgc gggcactctt
      421 cgtccagttc gcatgtgatc ccccggtgtt atcgttctcg cgacgcttca aagcgagggt
      481 aggcgaagca ccaccctcgg tggcgccaca gtctaccgaa gcggcggggg gagtctacgg
      541 gcccgaggtg gcggccgcgc aacaggtcag ccgggagccg ctccttgcgt gttcacatag
      601 accgcgtaga gcgattggct cgcggccatg aacagccggt tccgctttgg gccgccgaaa
      661 gccacgttcg cgcagacctc cggcaaccgg atgcggccca acagggcgcc cgctgaattc
      721 cagacggtga cgccgctata gccgacacgg agccccgcgt tgctcgagca ccagacgttg
      781 ccttggacat cgacgcgcac gccgtccgtg cggcatgcga cgccgtccac ggtgaggtcg
      841 aggaactgct tgacgcccga gacgcggccg tcgtcggcga cgtcgccgac gtgcagattg
      901 ccgccgctga cgacgtagac ctttttgtag tcgaccgaga aggcgaggcc gttcggcggg
      961 cccgggagga catcggccgc gatcgcgatt gcgactcggc cgttcggcgc aacgcgatag
     1021 acgttggtcg gcagctcgcg ctgcgcttcg ccgacgcccg ctcgttggcc gactcgctgc
     1081 cgcaggatgc cctgcgggtt gctaggcccg ccgggtgcgt ccggcactcc ctcgtagagc
     1141 tggccgccga acgccgggtc cgtgaaccag tagctgccgt ccggatgcgc gacgacgtcg
     1201 ttcggcgagt tcaggggctt gccgtcgtag gcgtccgcga gcacgacgac gctgccgtcg
     1261 ttctcgtagc gcacgacgcg ccgcaacaag tgctcgcacg tgacttgccg gccttggaag
     1321 tcgaacgtgt tgccggtgct gttgttcgtc ggcgagcgaa acacggtcac gcgcccgtcg
     1381 tcctcgagcc agcgcagctg gcggtcgttc ggaatgtcgc tccagacgag atatcggccc
     1441 tggctcgacc acgccggccc ctccgaccac agcgcgcccg tccagaggcg cacgatcggc
     1501 gtattgccga tgcgtaaggc attgaacgcg gggtcgacgg tcacgaggtc ggggtcggga
     1561 taggtgacag gctgcgcgcc ggggctgaaa tcgcgcggcg ggttcgagac cgtcgacggc
     1621 gggtccaccg gcgcttgcgc ggctagccag cccggcgcca gggctgcggc gccggccgcg
     1681 ctcatcgtct tgatcacgac gcgccgtgat cgttgcgcat cgccgtcgaa gtgctgatcg
     1741 ggcttgctca cgtgccgact ccctgcgttt cagaccccag tgtagagtag ccgtgcaggc
     1801 aaacgtcagg ggggagtccg tgcctaaatc gttgcgcgtc gtcttgccat gtctcgtcgc
     1861 cggtctcatg gcggcctgct cgccggctcc ggaaacgacc accgcggcaa aagagcccgc
     1921 cgccgctccg ccgccgacgg ctgccgcaca agccgccgcc aacgcggcgg gcccgacggt
     1981 caattgggcc tactacggcg gcaacctcgc ctcacagcgt tactcgccgc tcgagcagat
     2041 caacgccggc aacgcgggcc agctcaagat cgcctggcgc ttcagcacgg gcaactacgg
     2101 gccgcggccc gaagcgcgta acgaaacgac gccgctgatg atcgatggcg ttctgtacac
     2161 gcaggccggc acgacgcgca acgtcgtcgc cgtcgacgcg aagagcggcg agctgctctg
     2221 ggtctggcgc ccgaacgacg gcgagcagcg ctacgcgcgc gcgccccgca cgacgtcggg
     2281 tcgcggcccc gcgtactgga ccgacggcgc cggcaacgag cgcttgttca cggtcacgcc
     2341 gggtttcttc ctcgcggcgc tcgaccccga cacgggacgg cccgtgccgg gctttggcga
     2401 gaacggcatc gtcgacctca tggtcggcgt gcgcggcccc gaggctaccg acaagacgag
     2461 cattggcaac agctcgccgg cgctcgtgat cggcgatgtc gtcgtcgtcg gtcccgcgca
     2521 cgaggtcggc atgcggccgc cgtcgcagcg caacttcaaa ggcgacgtgc gcggcttcga
     2581 cgtgcatacg ggcaagctgc tctggacgtt ccacacgatt cccgaagccg gcgagttcgg
     2641 ctatgacacg tggctcgacg gctcggccga gcgcacgggt aacgccggcg tctgggctgc
     2701 gatgacggcc gacgaggagc tcggctacat ctaccttccc accgaggggg cgcttgcgga
     2761 catctggggc ggcgagcgtc acggcaacaa tttgttcagc tcgagcctcg tctgcctcga
     2821 cgcgaagacc ggcgagcgcg tttggcacta ccagctcatt caccacgaca tctgggattg
     2881 ggacaatccg acggcgccga tcttgatgga cctcgtcgtc gacggccggc cgatcaaggc
     2941 cgtcgcgcag ctcacgaagc aggcattcgt ctatacgttc gatcgcgtga cgggcgagcc
     3001 cgtctggccg atcgaggaac gtcccgtgcc gcagacggac gtgcccggcg agtggacgtc
     3061 gccgacgcag ccgttcccga cgaagccgcc gccgtacgac cggcaaggcg tctcggtcga
     3121 cgatctcatc gacttcacgc cggccttgcg cgccgcggcg atcgaaggcg tcaagaagtt
     3181 ccgcatcggg ccgattttca cgccgccgtc gctcgcgaac gcggccgacg gcacgagcgg
     3241 cacgctgacg ttgccgaacg cgacgggcgg cgcgaactgg gagggcggcg ccttcgatcc
     3301 cgagacgaac gtgctgtacg tcggctcagt cacgaacccg tcgatcttcg cgctcgagcc
     3361 gccgccgtca ccgaccgaca tcacctacat caatggcggc ggcgcccagc tgccctggtt
     3421 gaacggcttg ccgctgatca agccaccctg gggccggatc acggcctacg acatgaacaa
     3481 gggcgagatg ctctggcagc tagccagcgg tccgacgccg aaggcggtcg ccgagaattc
     3541 cgcgctcaag gggctcgaca tcccgccgac gggcagcgcg gcgcggcccc tcacgctcgt
     3601 gacgaagacg ctgttgttca cggccgacgg ctggggtgcg acgccggcgc tgcaggctcg
     3661 cgacaaagcg acgggagaaa tcgtcgccga ggtcgcgttg ccgggcgccg tcggcgggcg
     3721 gccgatgacc tacatgctgg acggcaagca gtacgtcgtc gtctcggtcg cggggagcaa
     3781 cggcgcggag atcgtggcgc tgacgctata gaaaaagctg catctaggct gccgccgatt
     3841 gccgcgtgcg ctgaccaggc cgacagctcg ctcgtctgcg acgagctgaa gagtccgctc
     3901 gatccgtaaa gagcgacgtc ggtgaatggc ggcgagtaga gcggcgccgg tccatcacgc
     3961 ccccgagctt cgtcagattc tccgcgagca ttccgggcgg ccgggtgacg cagtccgggc
     4021 cgagaatctg cgcgttgctg tcaaaaatcg ttattgggca gcgagttgtg ggctggataa
     4081 aataccagcg catccatccg cttcgccaat cggag
//
LOCUS       DATLEE010000105         2369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_859822, whole genome shotgun sequence.
ACCESSION   DATLEE010000105 DATLEE010000000
VERSION     DATLEE010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859822"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VM692_02320"
     CDS             <1..316
                     /locus_tag="VM692_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUQ51027.1"
                     /translation="DARRVARIFIVQGAAIGLVGALLGVGLGIAIAYNIDVIVPWLEQ
                     TFNFQIMPGDVYYVTALPSEVQLFDVIAVPTAAFALAILATLYPARRAAAIAPAIALR
                     YD"
     gene            323..1582
                     /locus_tag="VM692_02325"
     CDS             323..1582
                     /locus_tag="VM692_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HUQ51028.1"
                     /translation="MRQPYELSIAVRYLRTRSSHSFISFISAVSMIGIALAVAVLIVV
                     LSVMNGFEYELQRRILGMVSDASITGYDEPLADWQQARERVLQRDDVEAAAPYVEGQA
                     MAQAGETLAGITVRGIDPQLETQVSTIGSVMTSGSLDELTPGSFRVVIGRQLATLLGV
                     EVGDKVVLVLAEAWVTVAGLAPRLKRFTVSGIFDAGMYEYDRGLVFTSFDDAALLFGT
                     GGEATGLRLAIPDLYNSREVAMDAAKSLATERGGNLSIEDWTRLHYTFFRSIQLQKSM
                     MFVILSLVIAVAAFNIVSTLMMVVRDKRGDIAILRSFGTTQRSVMTLFASQGTFIGVV
                     GTLLGVLLALLISWQLGNVVNVLQGWFGIDLLSAEVYYLEDLPTQVRPLEVLQIGGMA
                     LVLAVAATFYPALSAARQPPAEALRYE"
     gene            1590..2309
                     /gene="lolD"
                     /locus_tag="VM692_02330"
     CDS             1590..2309
                     /gene="lolD"
                     /locus_tag="VM692_02330"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HUQ51029.1"
                     /translation="MDARSKAAQGAVAEAVAVLEARQLIKTFSDAGRNVEVLRGVDFS
                     VAKGDRIAIIGSSGSGKTTLLQLIGGLDQPTSGEVRVVGESMSTLRDAERGRLRNRAL
                     GFVYQFHHLLPEFTALENVAMPLLVRGAAPQQAAAEARGLLERVGLGERLEHRPGQLS
                     GGERQRTAVARALVTRPAAVLADEPTGNLDRPTGLRVFELLLELNAALGTSLVVVTHD
                     PDLAARMDRVLELKDGILRPA"
BASE COUNT          356 a          776 c          800 g          437 t
ORIGIN      
        1 cgacgcgcgg cgagtcgccc gcatcttcat cgtgcagggc gcggcgatcg gcctcgtcgg
       61 cgcgctgctc ggggtcggct taggtatcgc gatcgcctac aacatcgacg tcatcgtgcc
      121 gtggctcgag cagacgttca acttccagat catgcccggc gacgtctact acgtgacggc
      181 gttaccgtcc gaggtccagc tgttcgacgt catcgcagtg ccgacggccg cgttcgcgct
      241 cgcgatcctc gcgactctgt acccggcgcg gcgggcggct gcgatcgcgc cggcgatcgc
      301 actgcgttac gactagtcgc cgatgcgcca accttacgag ctgtcgatcg ccgtccgcta
      361 tctgcgtact cgcagcagcc acagctttat ctcgttcatc tcggcagttt cgatgatcgg
      421 catcgcgctc gctgtcgcgg tattgatagt cgtgctgtcg gtcatgaacg gcttcgagta
      481 cgagctacag cggcggatct tgggcatggt ctcggacgca tcgatcacgg gttacgacga
      541 gccgcttgcg gattggcagc aggcgcgcga gcgcgtgctg cagcgcgacg atgtcgaggc
      601 cgcggcgccc tacgtcgaag ggcaagcgat ggcccaggcc ggcgaaacct tggccggcat
      661 cacggtgcgc ggcatagacc cgcagctcga gacgcaggtg tcgacgatcg gctccgtcat
      721 gacgagcggc agcctcgacg agctgacgcc gggcagtttc cgcgtcgtga tcggccgcca
      781 gcttgcgacc ctgctcggcg tcgaagtggg cgacaaggtc gtgctcgtgc tcgccgaggc
      841 gtgggtcaca gtggcgggtc tagcgccgcg cttgaagcgg ttcaccgtca gcggcatctt
      901 cgacgcaggc atgtacgaat acgatcgcgg cctcgtcttc acgagcttcg acgacgcggc
      961 cctcttgttt gggacgggtg gcgaagcgac gggcttgcgg ctcgcgatcc ccgacctcta
     1021 caactcgcgc gaggttgcga tggatgcggc caagtcgctc gccaccgagc gaggcggcaa
     1081 cctgtcgatc gaggattgga cgcgactcca ttacacgttc ttccgctcga tccagctgca
     1141 aaagtccatg atgttcgtga tcttgtctct cgtgatcgcc gtcgcggcgt tcaacatcgt
     1201 ctcgacgctg atgatggtcg tgcgtgacaa gcgcggcgac attgcgatcc tgcgcagctt
     1261 cggcacgacg cagcgcagcg tcatgacgtt gttcgcgagt caaggcacgt tcatcggcgt
     1321 cgtcggcacg ttgctcggcg tgctgctcgc gctgttgatc tcgtggcagc tcggcaatgt
     1381 cgtcaacgtg ctgcaaggct ggttcggcat cgatctgttg tcggccgagg tctactactt
     1441 ggaggactta ccgacgcagg tgcgcccgct cgaggttctg cagatcggcg gtatggctct
     1501 cgtgctcgcc gtcgccgcga cgttctatcc cgcgttgagc gccgcgcggc aaccgccggc
     1561 cgaggcgctg cgctatgagt agtgccggca tggatgcgcg cagcaaggca gcgcagggcg
     1621 cggttgccga agcggtagcc gtcctcgaag cgcgccagct catcaagacg ttcagcgacg
     1681 cagggcgcaa cgtcgaggtg ctgcgcggtg tcgacttcag cgtcgcaaaa ggcgatcgca
     1741 tcgcgatcat cggctcgtcg ggctcgggca agacgacgct gctacagctg atcggcggcc
     1801 tcgatcagcc gacgtcgggc gaggtgcgcg tcgtcggcga gtcgatgagc acgctgcgcg
     1861 acgccgagcg cggccgcttg cgcaatcgcg cgctcggttt cgtctatcag ttccatcatc
     1921 tattgccgga gttcacggcg ctcgagaacg tcgcgatgcc gctgctcgta cgcggcgccg
     1981 ctccgcagca agccgccgcc gaagcgcgcg gcctgctcga gcgcgtcggc ttgggcgagc
     2041 ggctcgagca ccggccgggt caactctcgg ggggcgagcg gcagcgaacg gccgtcgcgc
     2101 gtgcgctcgt gactcggccc gcggccgtgc tcgccgacga gccgaccggc aacctcgacc
     2161 ggccgacggg tttgcgagtg ttcgagctct tgctcgagct caacgccgcg ctcggcacga
     2221 gtctcgtcgt cgtcactcac gaccccgatc tagcggcgcg catggaccgc gtgctcgagc
     2281 tcaaggacgg catattgagg cccgcctagc caacgctgcg ttcgccgtcg cacgccgtct
     2341 tcgtcccgca ccgatcgtcg tagcagggg
//
LOCUS       DATLEE010000106         4531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1606990, whole genome shotgun sequence.
ACCESSION   DATLEE010000106 DATLEE010000000
VERSION     DATLEE010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606990"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VM692_02335"
     CDS             <1..623
                     /locus_tag="VM692_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51030.1"
                     /translation="DRETGRPRYRPDILQSKVGEWIDACPSTAGGKNWHAMSFHAPTR
                     QLIIPLSQSCVSLRAQAIEQKPGGGSGGGADRRFYTMPGTNGNVGKLAAFNVDTLEQM
                     WSLEQRASFMTSVLSTAGGVAFAGDLDRTFKAVDVRDGRVVWQTRLATSVQGFPISFS
                     VDGKQYVAVATGLGGGSPRQVPATITPELRPPNRGQALYVFALPEP"
     gene            complement(625..1203)
                     /locus_tag="VM692_02340"
     CDS             complement(625..1203)
                     /locus_tag="VM692_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUQ51031.1"
                     /translation="MDCLLRHRSLALYLALMAGSTIPGVALSADNGAYVGAATSNVSS
                     DYGAPFGAGPVQDDDGLKLIAGFRPVDAFAIEANYADFGTTRASLNVACVTTPCPQEI
                     AIDSRALSVSAVGLFTLPLVDLYARAGVARWESERRVLSLSQKSADTDPTYGAGLQLR
                     LGSFALRVEYERFELGDESVDLRSVGFTYTFL"
     gene            1304..3406
                     /locus_tag="VM692_02345"
     CDS             1304..3406
                     /locus_tag="VM692_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51032.1"
                     /translation="MSRHSRHIGAAFAALTTTLASAQEVALSYTAEQAQQARGVYDST
                     CSTCHGPNFADGPLGAPLKGDAFMRKYGGKSARELFDVMRTTMPTGAPGSLPGETYAA
                     LVALLLQENLIVAGETPLPADPQRLAAMQIPAGGFSFMAFSPYTARANVDRPTPLTEF
                     KSVTDDSIAAPPPGDWLGWRRSYDAQGFSPLREIDARNVQSLRLAWSWTMPAGSAEGV
                     PVVRDGTIFVQSYGDIVQALDAKTGDLLWQYTHTLEQGASPFHKRGLALHGDRLYLGT
                     SDVHVVALDVATGEVVWDTKVGDFRKREGLNGGPLVARGKVMIGTTGTGVAAKIGGPQ
                     IVGLDEATGAEAWRLGTIAKPGEPGDESWNGIPFDERSGASLWTPGSYDPSTGLAYFG
                     TGNTYDTGPLLKPNAPGQTSDALYTNSTLAVNPDTGALVWHFQHFPNDQWDLDWAFER
                     QIMDLPVNGVMRRVVLTAGKIGIYDAVDARTGEFVFSIDLGLNNIVTEIDARTGEKHV
                     DRSRYPDGSVQLICPHGAGAKNFLPAAYIAARRTIVVPLNEACMDLFPVPGGGTGGLS
                     SGVNWGIRPRPDSDGNYGRLQAIDLATRETVWTTRQRAPQTSGVLATAGELVFAAAFD
                     RGFRAYDARTGRQLWETRLNDVSSSSPISYAVDGKQYVAIVVGEGGFHARSFAPLVPE
                     FRSPPNRGATLWVFALPN"
     gene            complement(3407..4048)
                     /locus_tag="VM692_02350"
     CDS             complement(3407..4048)
                     /locus_tag="VM692_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HUQ51033.1"
                     /translation="MSKLRVNSFSVSLDGYGAGPRQSRETPLGVGGENLHHWMFGTRT
                     FRQLFGREGGATGVDDDFARRGFANVGAWILGRNMFGPIRGAWLDNSWKGWWGEEPPY
                     RCQVFVLTNYRRKSFAMEGGTTFHFVTGGVEDALNRARKAADGKDVRVGGGVATIQQY
                     LRAQLLDELHLAFSPVLLGSGEALFAGIDLPALGYKVTEHVATPSATHVVLAK"
     gene            complement(4267..>4531)
                     /locus_tag="VM692_02355"
     CDS             complement(4267..>4531)
                     /locus_tag="VM692_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51034.1"
                     /translation="ATANEEHIEWLRPKGSMTAFPRLRGVADARPFCVAAAERGVLVA
                     VGAAFGAPAHFRIGFGLTMPRYAEALDILSDVLATSTWRQRAT"
BASE COUNT          776 a         1562 c         1493 g          700 t
ORIGIN      
        1 tcgaccgcga gaccggccgg ccgcgctacc ggcccgacat cttacagagc aaagtcggcg
       61 aatggatcga cgcgtgcccg agcacggccg gcggcaagaa ctggcacgcg atgagcttcc
      121 acgcgccgac gcgacaattg atcatccctt tgagccaaag ctgcgtctca ctgcgcgcgc
      181 aagccataga gcaaaagccc ggcggcggca gcggcggcgg cgccgatcgg cgtttctata
      241 cgatgcctgg cacgaacggc aacgtcggca agctcgcggc gttcaacgtc gacacgctcg
      301 agcagatgtg gtcgctcgag cagcgcgcgt cgttcatgac gtccgtgctg tcgacggccg
      361 gcggcgtcgc attcgccggc gatctcgacc gcacgttcaa ggccgtcgac gtccgtgacg
      421 gccgcgtcgt gtggcagaca cggctcgcaa cctccgtgca gggcttcccg atcagcttca
      481 gcgtagacgg caagcagtac gtcgccgtcg cgacgggctt gggcggcggc agcccgcgcc
      541 aagtgcccgc gacgatcacg ccggagctgc gcccgccgaa tcgcggtcag gcgctgtacg
      601 tcttcgcctt gccggagccg tgagctagag gaacgtgtag gtgaaaccca cggacctcaa
      661 atccacggac tcgtcgccga gctcgaagcg ctcgtactcc acgcgtagag cgaagcttcc
      721 caagcgcaac tgaagacccg cgccgtacgt aggatccgtg tctgcggatt tttgcgataa
      781 cgacagcacg cgtcgctccg actcccagcg agccacgccc gcgcgcgcat acaggtcgac
      841 gagcggcagc gtgaagagac cgaccgccga cacggacaac gcccgggagt cgatcgcgat
      901 ctcttgcggg cacggcgtcg tcacacaagc gacgttcagc gaggcgcgcg tcgtgccgaa
      961 atccgcgtag ttcgcctcga tcgcgaacgc gtcgaccggc cgaaagccgg cgatgagttt
     1021 gaggccgtcg tcgtcctgca cgggtccagc gccgaacgga gcgccatagt ccgacgatac
     1081 gtttgaagtc gcggcgccga cgtaggcccc gttgtccgca gaaagtgcga ctccggggat
     1141 ggttgaaccg gccatgagcg cgagatacag cgcgagggac cggtgacgaa gtaagcagtc
     1201 cattgccaac tcctttctcc agtggaggcc ctagcttatc tcaaggtaga gagcaggccg
     1261 agaatcgtta caatcctgcg caacgtcctt tcgcggggcc cttatgtcca ggcactcgag
     1321 gcacattggc gccgcattcg ccgcgctcac gacaacgctg gcgtcggcgc aggaagttgc
     1381 gctgtcgtac acggccgagc aagcgcagca ggcgcgcggt gtgtacgact cgacgtgctc
     1441 cacgtgccac ggcccgaact tcgccgacgg cccgctcggc gcaccgctga aaggcgacgc
     1501 gttcatgcgc aagtacggcg gcaagtccgc gcgcgagctg ttcgacgtca tgcgcacgac
     1561 gatgccgacg ggcgctccgg gctcgttgcc cggcgagacg tacgcggcgc tcgttgcgct
     1621 gctgctgcaa gagaacctca tcgtcgccgg cgagacgccg ctgccggccg atccgcagcg
     1681 gctcgcggcg atgcaaatcc cggccggcgg cttcagcttc atggcgttct cgccgtacac
     1741 ggcgcgcgcg aacgtcgacc ggccgacgcc gctgaccgaa ttcaaatcgg taaccgacga
     1801 ttcgatcgca gcgccgccgc cgggcgactg gctcggctgg cgccgcagct acgacgcgca
     1861 agggttcagc ccgttgcgcg agatcgacgc gcgcaacgtg cagagcttgc ggctcgcatg
     1921 gagctggacg atgcccgccg gcagcgccga aggcgtgccc gtcgtccgcg acggcacgat
     1981 cttcgtgcag tcgtacggcg atatcgtgca agcgctcgac gcgaaaaccg gcgacctgct
     2041 ctggcaatac acgcacacgc tcgagcaagg cgcatcgccg tttcacaagc gcggccttgc
     2101 gctgcacggc gatcgtctct acctcggcac gtcggacgtc cacgtcgtcg cgctcgacgt
     2161 cgcgacgggc gaagtcgtct gggacacgaa ggtcggcgac ttccgcaagc gcgaggggct
     2221 caacggcggg ccgctcgtcg cccgcggtaa agtcatgatc ggcacgacgg gcaccggcgt
     2281 cgcggcaaag atcggcggcc cgcaaatcgt cggcctcgac gaggcgacgg gcgccgaagc
     2341 ctggcggctc ggcacgatcg cgaaacccgg cgagcccggc gacgaaagct ggaacggcat
     2401 tccgttcgac gagcgcagcg gcgcctcgct ctggacaccc ggcagttacg acccctcgac
     2461 gggcctcgcc tacttcggca ccggcaacac ctacgacacg ggcccgttgc tgaagccgaa
     2521 cgcgccgggg caaaccagtg acgcgctcta cacgaactcg acgctggccg tgaaccccga
     2581 caccggcgcg ctcgtctggc acttccaaca ttttccgaac gaccagtggg acctcgactg
     2641 ggcgttcgag cggcagatca tggatttgcc cgtcaacggc gtcatgcgcc gcgtcgtgtt
     2701 gacggccggc aagatcggca tctacgacgc cgtcgacgcg cgcaccggcg agttcgtgtt
     2761 ctcgatcgac ttaggtctga acaacatcgt gaccgagatc gacgcgcgca cgggcgagaa
     2821 gcacgtcgac cgcagtcgct atcccgacgg cagcgtgcaa ttgatctgcc cgcacggcgc
     2881 cggcgcgaaa aacttcttgc cggccgccta catcgcggcg cgccgcacga tcgtcgtgcc
     2941 gctgaacgaa gcgtgcatgg atttgttccc cgttcccggc ggcggcacgg gcggcctgtc
     3001 gtcgggcgtc aattggggca ttcgcccgcg gcccgacagc gacggcaact acggtcgctt
     3061 gcaggccatc gatctcgcga ctcgcgagac ggtttggacg acgcggcaac gcgcgccgca
     3121 gacgtcgggc gtgctcgcga cggccggcga gctggtgttc gcagcggcgt tcgatcgcgg
     3181 cttccgcgcg tacgatgcac gcacgggccg gcaactttgg gagacgcgct tgaacgacgt
     3241 ctcgagctcg agcccgatca gctatgccgt cgacggcaag cagtacgtcg cgatcgtcgt
     3301 cggcgaaggc gggttccacg cgcgcagctt tgcccccctg gtgccggagt tccgcagccc
     3361 gccgaatcgc ggggcgacgc tctgggtgtt cgcgctgccg aactagctac ttggccagca
     3421 cgacatgcgt cgcgctcggc gtcgcgacgt gctcggtgac cttgtagccg agcgccggca
     3481 ggtcgatgcc ggcgaacaac gcttcgcccg agccgagcag caccggtgag aatgcgaggt
     3541 gcagctcgtc gagcagctgc gcgcgcagat attgctgaat cgtcgcgacg ccgccgccga
     3601 cgcgcacgtc cttgccgtcg gccgccttgc gtgcgcggtt cagcgcgtcc tcgacgccgc
     3661 ccgtgacgaa gtggaacgtc gtgccgccct ccatagcgaa cgacttgcgc cgatagttcg
     3721 tcagcacgaa cacctggcag cggtacggcg gctcttcgcc ccaccaaccc ttccagctgt
     3781 tgtcgagcca ggcgccgcgg atgggcccga acatgttgcg gcccaagatc cacgcgccga
     3841 cgttcgcgaa gccgcgccgc gcgaagtcgt cgtcgacgcc cgtcgcaccg ccttctcggc
     3901 cgaagagttg ccggaacgtg cgagtgccga acatccagtg gtggaggttt tcgccgccga
     3961 cgcccaacgg cgtctcgcgg ctctgcctcg ggcccgcgcc gtaaccgtcg agcgagacgc
     4021 tgaaactgtt cacgcgcagc ttggacatcg cgaacctcct gctactcgac gaaaatcgag
     4081 cttggcttgg acgtctcatc cttgcgtgtt cgagcggagc gcgtcaacgc ggcgtttgtc
     4141 gtcgggaacg ttcgtgaagc ccgactccgt gatcgcgagc cgcgtgccct tctggtcggc
     4201 tccagccgaa actcgacgcg cgtcttcggc gcgcttgcga agtcgcgata atcgtcgtgc
     4261 ccgtcttcag gttgctcgtt gtcgccacgt gctcgttgcc agcacgtcgc tcaaaatgtc
     4321 gagcgcttcg gcgtagcgag gcatcgtgag accgaacccg atcctgaagt gcgccggcgc
     4381 gccgaacgct gcccctaccg caacgagcac gccgcgctcg gcggccgcaa cgcaaaacgg
     4441 ccgcgcgtcg gcgacgccgc gcaagcgcgg gaacgccgtc atcgagccct tcgggcgcag
     4501 ccactcgatg tgctcttcgt tggcggtggc c
//
LOCUS       DATLEE010000107         5301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2350868, whole genome shotgun sequence.
ACCESSION   DATLEE010000107 DATLEE010000000
VERSION     DATLEE010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2350868"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /gene="pomA"
                     /locus_tag="VM692_02360"
     CDS             <1..714
                     /gene="pomA"
                     /locus_tag="VM692_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein PomA"
                     /protein_id="HUQ51035.1"
                     /translation="TAIVLGGSVTAFVDTASIVLVIGGTLLVTLMKFSFPQFLNATKV
                     AFKAFIYRVPEPEALIDTSIHLANQARASGLLALEEADVPDEFFKKALNLVVDGHDPA
                     VVRSILIKDLELTLKRHRDGQAIFKAIGDAAPAMGMIGTLIGLVQMLGSMNDPKQIAP
                     AMAIALLTTLYGAMIANVIAIPIADKLALRSTDERLAKSLIIDAVMSIQDGQHPRVMQ
                     DLLKNYLPKNRRAEEQRAA"
     gene            730..1593
                     /locus_tag="VM692_02365"
     CDS             730..1593
                     /locus_tag="VM692_02365"
                     /inference="COORDINATES: protein motif:HMM:NF006508.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotB family protein"
                     /protein_id="HUQ51036.1"
                     /translation="MHAELPELPEVRNGPPPWLITFADLMVLLMCFFVLMLSFSEMDA
                     EKFKIMSGSLNEAFGVQTDVTAADAPKGTSLEANEFSPAIPEPTAVNEVRQHTVDSDL
                     NTLDLGLELRLQELKAQEEAAAKQAQELRAVFSQEIDDGKLLIRQDGSNVVIQLLEKD
                     SFPSGSAALEPGSLASLAKVGALVRGMTGAITVAGHTDNVPIRNGGQYRSNWELSAAR
                     AASVGHELLAAGLEPTRLMVSGHADTQPRAANDTAANRALNRRVDITFANGRDRPGVW
                     TRATADAPPAR"
     gene            complement(1590..2237)
                     /locus_tag="VM692_02370"
     CDS             complement(1590..2237)
                     /locus_tag="VM692_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51037.1"
                     /translation="MLRAITFGLLVLPASAALAEGHSIGLKVGAFGLGAEYTHELTDR
                     LAVRGALYGSQLGFDVEESGIEYQADVVWDSLTAGIDFHPLKSALRLSLGIMKNDNAL
                     ELVSRPTTNQTIGDTTYTPAQIGTLTGSLHFDDTAPFAGLGWDWSRDARLFGMSLDIG
                     VVNQGDAVVTLRGNGNLLGNPAFEQDINAEAAELAAEGSDFDLVPFLSVGFQFRF"
     gene            2475..3161
                     /locus_tag="VM692_02375"
     CDS             2475..3161
                     /locus_tag="VM692_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51038.1"
                     /translation="MSLPLSDRPRLRWLILAAGACLAGCGGSQVIVESTFPTPLIEPL
                     PVSMGIIIPDELYNFIYTEDIPDQSLWTIALGDANVAMLEPLFKGMFRETRDVESLAA
                     ADRSLDGVIEPKLDKFEFDVPNGERDEFVEVWLQYQITVYDPDGGTVIQWPVSGYGKS
                     ELLRDPEDAVQRAAIVAMREAGATISTKFSEQPQVKQWLGGKGYAAPVTVGPQAAVGE
                     PATTEPAPIQ"
     gene            3163..3873
                     /locus_tag="VM692_02380"
     CDS             3163..3873
                     /locus_tag="VM692_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51039.1"
                     /translation="MALLRVIGIAAALPLLGACGITSRIEPYKQSDAVIADGEQVVIL
                     ARKQHSTHEAEEEFVQCLSDGLADGKHGLDVHSTQEFEDKMYPWFEPSTAPLSTDDLS
                     VLLERPGVLDQVRSTGVRYVVWVDGSTDRVASGGGITCAAGVGGAGCMGLAWWEDDSR
                     YDFTVWDIQDRSSEGTIFADVKGRSVMPAIIIPIPLVTRPQAHTCRTVTDQLRQFLTS
                     PEGYPSPGAPPPVAADVG"
     gene            3993..>5301
                     /locus_tag="VM692_02385"
     CDS             3993..>5301
                     /locus_tag="VM692_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUQ51040.1"
                     /translation="MSDRRDLELVLESGTPLIVIETTDEARVLELLRELALSRPASSY
                     RPLFRWSVTDGLQRLDLDLEAQRHNAEPEEALRHIRAVAKPGIYALLDFHPFLSEPVH
                     VRLLKDIALAANAAKVTLLLIGHRLELPQELAGFTARFALRLPDPNDRRAIIERCAIE
                     YEQESGRQVKVDKAALDLLIQNLAGLTTADTERLARNAIRDDGAVTASDLPAVMKAKY
                     QLLNRGGVLSFEYDTTRLADLAGFRNLKHWLKQRASAFRPDRPTGLEAPKGILLLGVQ
                     GCGKSLAAKATAGAFGLPLLRLDFGSLYNKYHGETERNLRESLQTAEVLSPCVLWLDE
                     IEKGLATGHDDSGTARRVLGGFLTWMAERRAQVFLVATANEVSELPPELIRKGRFDEI
                     FFVDLPPPTSRAEILTIHLRKRGLAPERMNLADLVTLTDGFSGA"
BASE COUNT          976 a         1732 c         1666 g          927 t
ORIGIN      
        1 acggccatcg tgctcggcgg ttccgtgacg gcattcgtcg atacggcgtc gatcgtgctc
       61 gtgatcggcg gcacgctgtt ggtgacgctg atgaaattca gctttccgca gttcttgaac
      121 gccaccaagg tcgccttcaa agcgttcatc taccgcgtgc ccgagcccga ggcgctgatc
      181 gatacgtcga ttcatctcgc gaaccaggcc cgtgcgagcg gcctccttgc cctcgaggaa
      241 gccgacgtcc ccgacgagtt cttcaagaag gctttgaacc tcgtcgtcga cggccacgac
      301 cccgccgtcg tgcgctcgat cttgatcaag gatctcgagc tgactctgaa aaggcatcgc
      361 gacggccaag cgatcttcaa ggcgatcggc gatgcggcac cggccatggg catgatcggc
      421 actctgatcg gtctcgtgca gatgctgggc tcgatgaacg acccgaagca gattgcgccc
      481 gccatggcga tcgcgctgtt gacgacgctg tacggtgcca tgatcgccaa cgtcatcgcg
      541 attccgatcg ccgacaagct cgcgctgcgc agcacggatg agcgactcgc caagtcgctg
      601 atcatcgatg ccgtcatgag catccaggac ggccagcacc cgcgcgtcat gcaagacctg
      661 ctgaagaact atctgccgaa gaaccggcgc gccgaggaac agcgggctgc ctgagcccgc
      721 tacttcaacg tgcacgccga gctccccgag ctccccgaag ttcgcaacgg tccgccgccg
      781 tggctgatta ctttcgcgga cctcatggtc ctgctgatgt gcttcttcgt cctgatgttg
      841 tcgttctccg agatggacgc ggagaagttc aagatcatgt cgggctcgct gaacgaggcg
      901 ttcggcgtgc aaacggacgt cacggcggcc gacgcaccga aaggcacgag cctcgaagcc
      961 aatgagttca gccccgcgat ccccgagccg acggccgtga acgaggttcg ccaacatacg
     1021 gtcgattcgg atttgaacac gctcgatctc gggctcgagc tgcggctgca ggagctcaag
     1081 gcgcaagagg aagcggctgc taagcaagcg caagagctgc gcgccgtgtt cagtcaagag
     1141 atcgacgacg gcaaactcct gattcgtcaa gacggcagca acgtcgtgat ccagttgctc
     1201 gagaaggact cgttcccgtc gggaagcgcg gcactcgagc ccggctcgct cgcatcgctc
     1261 gccaaggtag gcgcattggt ccgcggcatg acgggggcaa ttaccgttgc cgggcacacc
     1321 gacaatgtgc cgatacgcaa cggcggccaa taccgctcca actgggagct ctcggcagcg
     1381 cgcgcggcgt cggtcggcca cgagctattg gccgcgggcc tcgagccgac gcgcttgatg
     1441 gtcagcgggc acgccgacac tcagccgcgc gctgcgaacg atacggcggc gaatcgcgcg
     1501 ttgaaccggc gcgtcgacat cacgtttgcc aacggccgcg atcggccggg cgtctggacg
     1561 cgggcaactg cggacgcccc gcccgctcgt tagaagcgga actgaaaccc gaccgacaga
     1621 aacggcacga gatcgaagtc cgagccctcg gcggcgagct ccgcggcttc tgcattgatg
     1681 tcttgctcga acgccgggtt gccgagcagg ttgccgttgc cgcgcagcgt gaccacggcg
     1741 tcgccttgat tcacgacgcc gatgtcgagc gacatgccga acaggcgcgc gtcgcgcgac
     1801 caatcccagc ctaggcccgc gaacggcgcc gtatcgtcga aatggaggct acccgtcagt
     1861 gtgccgatct gcgccggcgt gtacgtcgta tcgccgatcg tctggttggt cgtcgggcgg
     1921 ctcacgagct ccaaagcatt gtcgttcttc atgatgccga ggctgagacg caaagccgac
     1981 ttcagcggat ggaagtcgat gccggccgtc agcgagtccc agaccacgtc ggcttggtac
     2041 tcgatgccgg actcttcgac gtcgaacccg agctgcgagc cgtagagcgc cccgcgcacg
     2101 gcgagcctat cggtcagctc gtgcgtgtac tcggcgccga gcccgaacgc acctactttg
     2161 aggccgatgg aatgcccctc agcaagcgct gccgacgctg gcaggaccaa cagaccaaat
     2221 gtgattgcgc gcaacatgat tcgctccaaa gctcgctcag ttgccacggt agtcggcgta
     2281 accataactc gcgactgaac gcgtcacatt tacggctgtt ccgggatcgg cgccaaacgt
     2341 tcaatgtctc atgaattttt cgggcgcggc gcggtctgag cttcgtgtcg gagctagctc
     2401 tgttggccac aatcgttcgg tgttaacgtt gaatcagggc gcaatttgcg tacccaggac
     2461 gtaagattca ggtcatgtcc ctgccgttgt cagatcgccc gaggctccgc tggctgatcc
     2521 ttgccgccgg tgcgtgcctc gcgggatgcg gcggctccca agtcatcgtc gaatcgacgt
     2581 tcccgacgcc gcttatcgag ccgttgcccg tcagcatggg catcatcatt cccgacgagc
     2641 tctacaactt catctacacg gaagacattc ccgatcagtc gctgtggacc atcgcactcg
     2701 gcgatgccaa cgtcgcgatg ctcgagccgt tgttcaaagg aatgttccgc gagacgcgcg
     2761 acgtcgagtc gttggccgcc gcagaccgca gcctcgacgg cgtgatcgag ccgaagctcg
     2821 ataaattcga gttcgacgtt ccgaacggcg agcgtgacga gttcgtcgaa gtctggctgc
     2881 aataccaaat cacggtgtac gaccccgatg gcgggaccgt gattcagtgg cccgtctcgg
     2941 gctacggtaa atccgagctg cttcgcgacc cggaagacgc ggtgcagcgc gccgccatcg
     3001 tggcgatgcg cgaagcgggc gcaacgattt ccaccaagtt cagcgagcaa ccgcaagtca
     3061 aacagtggtt agggggtaaa ggttatgccg cacccgtcac agtcggaccg caggcagccg
     3121 tcggcgagcc cgccacgacc gaacccgcgc ccatccaata acatggcatt gctccgcgtc
     3181 atcggcatcg ccgccgccct accgctgctc ggagcttgcg gcatcacgtc gcgcatcgag
     3241 ccgtacaagc agagcgacgc cgtcatcgcc gacggcgagc aagtcgtgat tttggcgcgc
     3301 aagcagcatt cgacgcacga agccgaagaa gaattcgtgc agtgcttgag cgacggcttg
     3361 gccgacggca agcacgggct cgacgtccac tcgacgcagg agttcgagga caagatgtat
     3421 ccgtggttcg agccttcgac cgcgccgttg agcactgacg acttgtcggt gctgctcgag
     3481 cgccctggcg tgctcgacca agttcgcagc accggagttc gctacgtcgt ctgggtcgac
     3541 ggctcgacgg atcgcgtggc cagcggcggc ggcatcacgt gcgccgccgg cgtcggcggt
     3601 gccggttgca tgggtctcgc atggtgggaa gacgactcgc gctacgactt caccgtctgg
     3661 gacattcaag atcgctcgtc cgaaggcacg atcttcgccg acgtcaaagg ccgctccgtg
     3721 atgccggcaa tcatcatccc gatcccgctc gtcacgcgcc cgcaagctca cacgtgccgt
     3781 accgtcactg atcagctgcg acagttcttg acctcgccgg aaggctaccc gtcgccgggc
     3841 gcgccgccac cggtggccgc agacgtcggc taaccgcacg ccgcccgagc gaatctgccg
     3901 actcggtcgc catggcgcga ccttcgtcgc tctagactag cggctgcgtt ttttcacatc
     3961 ggggccgcct gcatcacggc aggcctgcag tgatgagcga ccgccgcgac ttagagctcg
     4021 ttctcgaatc cggaacgccg ctgatcgtca tcgaaaccac cgacgaggcg cgcgtcctcg
     4081 agctattgcg cgagcttgcg ttgagtcgcc cggcaagctc ttaccggccg ctgttccgct
     4141 ggtccgtcac cgacgggttg caacgactcg acctcgatct cgaggcgcag cgccacaacg
     4201 ccgagcccga ggaagcgctc cgccacatcc gagccgtcgc caagccgggg atctacgcgt
     4261 tgctcgactt tcatccgttc ttgagcgagc ccgtgcacgt gcggctgctc aaggacatcg
     4321 cgctggcggc gaatgcggcg aaggtcaccc tgttgctgat cggtcaccgg ctcgagctgc
     4381 cgcaggagct cgcgggcttc acggcgcggt tcgcgttgcg cttgccggac ccgaacgaca
     4441 gacgcgcgat catcgagcgc tgcgcgatcg agtacgagca agaaagcggc cgccaggtga
     4501 aggtcgacaa ggcggcgctc gatctgctga tccagaattt ggcgggactc acgaccgcgg
     4561 acacggagcg cctcgctcgc aacgccattc gcgacgatgg tgccgtgacg gccagcgatc
     4621 tgccggcggt catgaaggcc aagtaccagc ttctgaaccg cggcggcgtg ctgagcttcg
     4681 agtacgatac gacgcggctg gcggacctcg ccggctttcg caacctcaag cactggctca
     4741 agcaacgcgc ctcggcgttc aggcccgatc gccctaccgg cctcgaagcg ccgaagggaa
     4801 tcctgctgct cggagttcag ggttgcggca agagtctcgc ggcgaaggcc acggccggcg
     4861 cgttcggttt gcccctcctg cggctggact tcggctcgct gtacaacaag taccacggcg
     4921 agacggagcg caatttgcgc gaatcgctgc aaacggccga agtcctgagc ccgtgcgtcc
     4981 tgtggctcga cgagatcgag aagggtctgg cgaccggtca cgacgacagc ggaacggcgc
     5041 gccgggttct cggcggcttt ctcacctgga tggccgaacg acgcgcgcag gtctttctgg
     5101 tcgcgacggc caacgaggtc agcgagctac cgccggagct gatccgcaag ggccgatttg
     5161 acgaaatctt cttcgtggac ttgccgccac cgacgagccg cgccgagata ctgacgatcc
     5221 acttgcgcaa gcgcggactc gctccggagc gtatgaacct cgccgatctc gtgacgctga
     5281 ccgacgggtt ttcaggcgcc g
//
LOCUS       DATLEE010000108         3229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9747548, whole genome shotgun sequence.
ACCESSION   DATLEE010000108 DATLEE010000000
VERSION     DATLEE010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9747548"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VM692_02390"
     CDS             complement(<1..1036)
                     /locus_tag="VM692_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51041.1"
                     /translation="MHLPKNRRRLGTALSLAALVVASSSTARSQTADGDWPMYSLDLG
                     GSRFSPLTELTPRNVSRLAEAWAAPVAREADDDSDAPGGRGNPQATPIVVDGVLYLPV
                     RGNEVLALEAHTGKELWRHTLRAPLSTTARGVAYWPGDGTRGPRILLTAGPTLVALDA
                     RTGRPAAGFGTDGVVQIAVPWNGVPTIYRDLAIVGATTNEIALAEAGDTRAFDVRTGK
                     MVWAFHTVPLPGEVGHDTWLDNGWRNRSGVNVWAWYLTLDSSRDILYMPLGSAAGNYW
                     GGDRPGDNLFGNSIVAVQATTGRYLWHFQTVHHDLWDLDLPNPPVLVDLVQGRERVPA
                     LASIGKTAWLF"
     gene            complement(1120..2535)
                     /locus_tag="VM692_02395"
     CDS             complement(1120..2535)
                     /locus_tag="VM692_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HUQ51042.1"
                     /translation="MTAPFTPSAGKDLLGHPRGLWYLVFAEAWERFSYYGMQALLVLY
                     LTQYLLVSGNVERIAGFAAFRNFLEGLYGPLATPVTLAAAITGLYSAGVYFTPLIGGY
                     ISDQWLGRTKSVAAGALLMVAGHFLMAFELAFVGAIACLFLGVGLFKGNIATQVGELY
                     GPDDLRRSTAFQTFFLGIQIAVIVAPLVIGFLGEKVAWHYGFGAAGVGMAIALVVYLS
                     GRKYLPPEQPRRAAAERAPAAKLTRRDWATMGVLIALLPVLGATAIGNQEIFNAYLLW
                     GEANYNLVFFGETMPVSWLVSLDAIISTATVGGAIVFWTVYARHWKEPSEMAKLAIGA
                     AIAAGGPLLLAIASANAAENGAKVSLVWGLGFHIVNDIGFAMLFPVGLALYSRAAPKQ
                     VGGLFIGIYYLHLFVCNFATGKVAGFIETMSGFNFWAMHAAIVFGGALALVVFAVLFG
                     KLLAPTGDEAPTPAAAHPAAA"
     gene            2733..2975
                     /locus_tag="VM692_02400"
     CDS             2733..2975
                     /locus_tag="VM692_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51043.1"
                     /translation="MGRERKTFVVDYEHAGEQLVAICKACQRTAILSFRELNRRGRHM
                     WTLAELARALRCSNCRKKVAEVRLQRSFVRPPANRH"
     gene            2977..3198
                     /locus_tag="VM692_02405"
     CDS             2977..3198
                     /locus_tag="VM692_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51044.1"
                     /translation="MNAHHYGPDDVKPGDWIKYRSSHYGSGRLAEVHAVGADAHSGPY
                     AVTSEGNVLFSRILEIRRRPINVVVFPQT"
BASE COUNT          604 a         1090 c         1065 g          470 t
ORIGIN      
        1 cgaacagcca cgccgtcttg ccgatcgacg cgagcgccgg aacgcgctcg cgaccctgca
       61 cgagatccac gagcacgggc gggttcggca ggtcgaggtc ccaaaggtcg tgatggaccg
      121 tttggaagtg ccagagataa cgcccggtcg tcgcctgcac tgcgacgatc gagttgccga
      181 acagattgtc gccgggccgg tccccgcccc agtagttgcc ggccgcgctg ccgagcggca
      241 tgtaaagaat gtcgcgcgat gaatcgagcg tgaggtacca cgcccagacg ttgacgcctg
      301 agcgattgcg ccagccgttg tcgagccacg tgtcgtgacc gacttcgccg ggcagcggca
      361 cggtgtggaa cgcccacacc atcttgcccg tgcgcacgtc gaacgcgcgc gtgtcgccgg
      421 cctcggcgag cgcgatctcg ttcgtcgtcg cgcccacgat cgcaaggtcg cggtagatcg
      481 tcggcacgcc gttccacggc acggcaatct gcacgacgcc atccgtgccg aaccccgcgg
      541 cggggcggcc cgtgcgcgca tcgagcgcga cgagcgtcgg cccggccgtc agcagaatgc
      601 gcgggccccg cgtgccgtcg ccgggccagt acgcgacgcc gcgcgccgtc gtgctcagcg
      661 gcgcacgcag cgtgtggcgc cagagctcct tgccggtgtg cgcctcgagc gcgagcacct
      721 cgttgccgcg cacgggcaga tagagcacgc cgtcgacgac aatcggcgtc gcctgcggat
      781 tgccgcgccc accgggcgcg tcgctgtcgt cgtcggcttc gcgcgcgacc ggcgcagccc
      841 acgcttcggc gagccggctg acgttgcgcg gcgtgagctc cgtcagcgga gagaaacggc
      901 tgccgcctaa atcgaggctg tacatcggcc agtcgccgtc ggccgtttgc gaccgcgccg
      961 tcgaagatga agccacaacc agagccgcga gcgagagtgc cgtgccgagt cgtcggcgat
     1021 tcttaggaag gtgcatccgg ggcccgccgc ttgggaaacg cgttgtcatt gagccgatga
     1081 aacgccaccg ggtcaatgtg ccgcggatcg gcgcggtact taggcagcag ccgggtgcgc
     1141 cgcggcgggc gtcggcgcct cgtcgcccgt cggcgcgagc agcttgccga acagcacggc
     1201 aaagaccacg agcgccagcg cgccgccgaa cacgatggcc gcgtgcatcg cccagaagtt
     1261 gaagccgctc atcgtctcga tgaagccggc gactttgccg gtggcgaagt tgcaaacgaa
     1321 caggtgcaga tagtagatgc cgatgaaaag cccgccgact tgcttcggtg ccgcgcgcga
     1381 gtacagcgcc aagccgacgg ggaacagcat cgcgaaaccg atgtcgttga cgatgtggaa
     1441 gccgaggccc cacaccaacg acactttcgc gccgttctcg gcggcattgg cgctcgcaat
     1501 cgcgagcagc agcggcccgc cggccgcgat cgccgccccg attgcgagct tggccatctc
     1561 gctcggctcc ttccaatgac gcgcgtagac ggtccagaac acgatggcgc cgccgaccgt
     1621 cgccgtggag atgatcgcgt cgagcgacac gagccacgag acgggcatcg tctcgccgaa
     1681 gaacacgagg ttgtagttcg cttcacccca aagcaggtag gcgttgaaga tctcttgatt
     1741 gccgatcgcc gtcgcaccga gcacgggcag cagcgcgatc aacacgccca tcgtcgccca
     1801 atcgcggcgc gtcaacttcg ccgccggcgc gcgctcggcg gccgcgcggc gcggctgttc
     1861 cggcggcaaa tatttacggc ccgagagata cacgacgagc gcgatcgcca tgcctacgcc
     1921 ggccgcgccg aagccgtagt gccaggccac cttctcgccg agaaagccga tgacgagcgg
     1981 cgcgacgatc accgcgatct gaatgccgag gaagaacgtt tggaacgccg tgctgcggcg
     2041 caaatcgtcc ggcccgtaaa gctccccgac ctgggtcgcg atgttgccct tgaagaggcc
     2101 cacgccgagg aacaagcacg cgatcgcgcc gacgaacgca agctcgaacg ccatcaggaa
     2161 gtgaccagcg accatcagca gcgccccggc cgcgacggac ttcgtgcggc ccagccattg
     2221 gtcggagatg tagccgccga tcagcggagt gaaatacacg cccgctgagt agagtcccgt
     2281 gatggcggcc gcgagcgtca cgggcgtcgc gagcggcccg tagagacctt ccaagaagtt
     2341 gcgaaaggct gcgaagcccg cgatgcgctc gacgttgccc gacaccagca ggtactgcgt
     2401 caggtagagc acgagcagcg cctgcatgcc gtagtacgag aaacgttccc acgcctccgc
     2461 gaacacgagg taccaaagcc cgcgcggatg gccgagcaga tccttaccgg cagacggcgt
     2521 gaaaggagcg gtcatgggca gcagtcgctc actgctgcga gtttagatca gcgggagcga
     2581 ccgaggtttg agtgtgccac agcgcggccg ctcctacgca atctcgaagc ttccgttgca
     2641 atcgcccacg accaacccaa gcgcgtcgct gccgcgagca ccgaagttac cgttatcgac
     2701 ttgagtctca atgcctgtat ataaaaccag gcatgggccg tgagcgcaag accttcgtcg
     2761 tcgactatga gcacgcgggc gaacagctcg tcgcaatctg caaagcctgt cagcgcaccg
     2821 cgatcttgag cttccgcgag ctcaaccggc gcggtcgcca catgtggacg ctcgccgagc
     2881 tcgcgcgggc gctgcgttgc agcaactgcc gcaagaaagt cgccgaggtg cggctgcaac
     2941 ggtcgttcgt gcgccctccg gcgaatcgac attgacatga acgcgcacca ctacggcccc
     3001 gacgacgtga agcccggcga ctggatcaag taccggtcga gtcactacgg cagcggacgg
     3061 ctcgccgagg tgcacgccgt cggcgccgac gcgcattcgg gaccgtacgc cgtcactagc
     3121 gagggcaacg tgctgttcag ccgcatcctc gagattcggc gtcggccaat caacgtagtg
     3181 gtgtttccgc aaacatgagc actagaattc gtcgcgtcga cgccgcagg
//
LOCUS       DATLEE010000109         2438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4585837, whole genome shotgun sequence.
ACCESSION   DATLEE010000109 DATLEE010000000
VERSION     DATLEE010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4585837"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2270
                     /locus_tag="VM692_02410"
     CDS             <1..2270
                     /locus_tag="VM692_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ51045.1"
                     /translation="HNATDQVAGLNLEALSFDAVASDAETWEHVIRKLRAGLMPPADG
                     GPTLDRETRTKLVAWLENEIDEHAETHLPPPGLHRLNRTEYTNAIRDLLALNIDAATF
                     LPADDSSHGFDNMAGTLTTSPALMEAYLSAAGKISRLAIGTETAPTLAVFDAPHDTSQ
                     NGYIEGLPFGTRGGMLIEHEFPADGNYTFTVKGMTGYFTAVLGNVSGEQLEVTVDGAR
                     VYLFDWDKEIKPNEGNGGKTPDIPIKAGFHRIGVTFLATSDLPDTGLNKSFVRTMNSP
                     GAISGYTFYPHVGQVFIEGPFDGAVAASTPSRDKIFECYPKESSPQESNEETECARKI
                     VSTLASKAFRRPATDADLDTMLEFFRAGRDDGGSFDYGVEAVVQRILADPEFIYRSEL
                     EPADVAPGQPYRISDLELASRLSFFLWSSIPDEELIKLASEGRLHETKVLELQVARMI
                     ADPRAAAFVENFTGQWLNVRGMRAAEPVVDLFPDFDSTLRDAFRREIELFFGSGIQED
                     RSILDLLTADYTFVNERLAKHYGIPDIYGPQFRRVTLPPELDMRRGLLGKGALLTITS
                     DAARTSPVKRGKWFLETFLDISPPDPPPGVETDLKVAPGEAPKTLRARMEAHRVNPSC
                     ASCHKLFEPLGLAMENFDGVGAWRTTDAGQPIDTAGVTNDGIPLTGLASLREFALGNG
                     DVFAQSVAERLLTYAIGRGLEYDDMPVVRSITRRAAEHDYKFSSLLMGVVESPAFTMN
                     VKSAVDQQTTAANRE"
BASE COUNT          432 a          839 c          793 g          374 t
ORIGIN      
        1 gccacaacgc gaccgatcag gttgcgggtc tgaacctcga ggcgctcagc ttcgacgccg
       61 tcgcctcgga cgccgagacg tgggagcacg tgatccgcaa gctgcgcgcc ggtctgatgc
      121 cgcccgcgga cggcggcccg acgctcgacc gcgagacgcg caccaagctg gtcgcctggc
      181 tcgagaacga aatcgacgag cacgccgaga cgcacctgcc gccgccgggc ctgcatcggc
      241 tgaaccgcac cgagtacacg aacgcgatcc gcgacctgtt ggcgttgaac atcgacgccg
      301 cgacgttcct gcctgccgac gactcgagcc acggcttcga caacatggcc ggcacgctga
      361 ccacgtcgcc cgcactgatg gaggcctacc tgtccgcggc cggcaagatc agccgcctcg
      421 cgatcggcac cgagacggca ccgacgctcg cggttttcga cgcgccgcac gacacctcgc
      481 agaacggcta catcgaaggg ctgccgttcg gcacgcgcgg cggcatgctg atcgagcacg
      541 agtttccggc cgacggcaac tatacgttca ccgtgaaagg catgacgggc tacttcacgg
      601 ccgtgctcgg caacgtgagc ggtgagcaac tcgaggtcac ggtcgacggc gcccgcgtgt
      661 atctcttcga ttgggacaaa gagatcaagc cgaacgaagg caacggcggg aagacgccgg
      721 atatcccgat caaggccgga ttccaccgca tcggcgtcac gttcctagcg acgagcgact
      781 tgcccgacac cggcctcaac aagtcgttcg tgcgcacgat gaactcgccg ggcgcaatct
      841 cgggctacac gttctacccg cacgtcggcc aggtcttcat cgaggggccg ttcgacggcg
      901 ccgtcgccgc aagcacgccg agccgcgaca agatctttga gtgctatccg aaagagagca
      961 gtccccaaga gagcaacgaa gagaccgagt gcgcccgcaa gatcgtatcg acgctggcta
     1021 gcaaagcgtt ccgccgcccg gcgaccgatg ccgatctcga tacgatgctc gagttcttcc
     1081 gcgcggggcg tgacgacggc ggcagcttcg actacggcgt cgaggccgtc gtgcagcgca
     1141 tcctcgcgga cccggagttc atctaccggt ccgagctcga gccggccgac gttgcgcctg
     1201 ggcagccgta ccgcatcagc gacctcgagc tcgcgtcgcg gctgtcgttc ttcctttgga
     1261 gcagcatccc cgacgaggag ctgatcaagc tcgcgtccga gggccgcctg cacgagacga
     1321 aggtgctgga gcttcaggtg gcgcgcatga tcgccgaccc acgcgcggca gcgttcgtcg
     1381 aaaacttcac gggtcagtgg ctcaacgtcc gcggcatgcg cgccgccgag cccgtcgtgg
     1441 acttgttccc ggacttcgat agcacgctac gtgatgcgtt ccggcgcgag atcgagctgt
     1501 tcttcggcag cggcattcag gaagaccgca gcatcctcga cttgctgacg gccgactaca
     1561 cgttcgtcaa cgagcggctc gcgaagcact acgggattcc ggacatctac gggccgcagt
     1621 tccggcgagt gacgttgccg ccggagctcg acatgcgccg cggcctgctc ggcaagggcg
     1681 cgttgctgac gatcacgtcg gacgcggcgc gcacgtcgcc cgtcaagcgc ggcaagtggt
     1741 tcctcgagac gttcctcgac atcagcccgc cggacccgcc gcccggcgtc gagaccgatc
     1801 tcaaagtcgc gcccggcgag gcgccgaaga cgttgcgcgc ccgcatggaa gcgcatcgcg
     1861 tcaacccgag ctgcgcctcg tgccacaagc tgttcgagcc gctcggcctc gcgatggaaa
     1921 acttcgacgg cgtcggcgcg tggcgcacga cggacgcagg ccagccgatc gatacggccg
     1981 gcgtgacgaa cgacggcatt ccgctgacgg gcctcgcgag cctgcgcgag ttcgcgctcg
     2041 gcaacggcga cgtgttcgcc cagtccgtcg ccgagcgact gttgacctat gcaatcggtc
     2101 gcggcctcga atacgacgat atgccggtcg tgcgttcgat cacgcgtcgc gcggccgagc
     2161 acgactacaa gttttcgtcg ttgctgatgg gcgtcgtcga gagccccgcc ttcacgatga
     2221 acgtgaagag cgccgtggat cagcagacga ccgcggcgaa tcgggagtag acccatgttc
     2281 ctgaccaaga agcacatcga tcgacgcacg ctgctgaaag gcgccggcgt cgcgttgggg
     2341 ttgccgctgc tcgagtcgat gttcccggcg gcgacggcgc tcgcgcacgc gaccgcggct
     2401 ccgccgcgcc gcttcttcgc cggcttcgtg ccgcacgg
//
LOCUS       DATLEE010000110         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9056200, whole genome shotgun sequence.
ACCESSION   DATLEE010000110 DATLEE010000000
VERSION     DATLEE010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9056200"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1448)
                     /locus_tag="VM692_02415"
     CDS             complement(54..1448)
                     /locus_tag="VM692_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HUQ51046.1"
                     /translation="MGKKRTISRRSLLRDSAAAAAGGAALLAQVGTAVGQAPAVIMDR
                     PFKAWISRGDGPGRTTLHEAKLRPIGGRQVLVRTEATNLCYSNVPAVLGLPPPTPPTA
                     SALAAPVAPAGRRMADMAVIQGHGGVGIVEAVGPEVRRVRVGDRVCVSGTPHCGSCYR
                     CLRGRSDMCQFLSAIGADDLVAIADLADGTPVYQNSHIGGLAEVMVAFEEWVVPIFTQ
                     LPAAELGMVCGCTSVAGLGATTAHALMTIEPASSVAVVGCGPLGLSAVQGARIAGAST
                     IIAIDPIRARREVALKVGATHALDPNVEGDGLVARVRALTTWPTDRLWAGGRNPAGRR
                     PGAGADFVVEAAGLDQVTPKLERGPDPTGLKPMQQAYQMCALGGHLITTSIIRGDMVL
                     PGNLFSIGGVTHHGGQAGGCSPMKDIPRFVELLEKGQYDAKTLATTVVPLERMLEGYE
                     EVAYRTTITGIMTA"
     gene            1602..>2259
                     /locus_tag="VM692_02420"
     CDS             1602..>2259
                     /locus_tag="VM692_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HUQ51047.1"
                     /translation="MNKHPQELRFSPLARAISHVFAGVSTLLFVGYATAQQPPAADEP
                     EEIVVTGSRIQRTSGFATAVPVTAVTRDDLTNFQPGRTMADQLDQLPQFFATQSAQRG
                     GGALFGSAGISTLNLRAMGPQRTLVLVDGIRVAPADRDGSVHIDNIPSALLSQVEVVT
                     GGASAAYGADALAGVVNFRINRQFEGLDFNVGTGRTAAGFGDQVDAAAAYGTPFGDKW
                     H"
BASE COUNT          357 a          788 c          744 g          370 t
ORIGIN      
        1 cagatagtat tagctcatgt gctaattgtc aaataagctg tctggcggtc gacctaagcg
       61 gtcatgatgc ccgtgatcgt cgtgcggtaa gcgacctcct cgtagccttc gagcatgcgc
      121 tcgagcggca cgacggtcgt cgcgagcgtc tttgcgtcgt actgcccttt ctcgagcaac
      181 tcgacgaagc gcggtatgtc tttcatcggg ctgcagccgc cggcttggcc gccgtgatgc
      241 gtcacgccgc cgatcgagaa cagattgccg ggcaggacca tgtcgccgcg gatgatgctc
      301 gtggtgatca gatgcccgcc gagcgcgcac atttgatacg cctgctgcat cggcttcaaa
      361 cccgtgggat cgggcccgcg ctcgagcttc ggcgtgactt gatcgagccc cgccgcttcg
      421 acgacgaagt cggcgcccgc tccgggccgg cgcccggccg gattgcgtcc gcccgcccag
      481 aggcgatccg tcggccacgt cgtcagcgcg cgcacgcgcg cgacgagacc gtcgccttcg
      541 acattcggat cgagcgcgtg cgtcgcgccg accttcagcg cgacctcgcg gcgcgcgcgg
      601 ataggatcga tcgcgatgat cgtcgacgca cccgcgatgc gcgcgccctg cacggcgctc
      661 aagcccagcg gcccgcaacc gacgacggcg acgctcgacg ccggctcgat cgtcatcagc
      721 gcgtgggccg tcgtcgcgcc gagacccgca acgctggtac agccgcaaac catgccgagc
      781 tccgccgcgg gcaactgcgt gaaaatcggc acgacccatt cttcgaacgc caccatgact
      841 tcggcgaggc cgccgatgtg cgagttctga tagacgggcg tgccgtcggc gaggtctgcg
      901 atggccacga ggtcgtcggc gccgatcgcg ctcaagaact gacacatgtc ggagcgcccg
      961 cgcagacacc ggtaacagga accgcagtgc ggcgtgccgg agacgcagac gcgatcgccg
     1021 acgcgcacgc ggcgcacctc gggcccgacg gcttcgacta tgccgacgcc gccgtgccct
     1081 tggatgacag ccatgtcggc catgcgccgc ccggccggcg cgaccggtgc ggcgagtgcg
     1141 ctcgcggtcg gcggcgtcgg cggcggcagg ccgagcacgg ccggcacgtt cgagtagcag
     1201 aggttcgtcg cctcggtgcg gacgaggacc tgacggcccc cgatcggtcg aagcttcgct
     1261 tcgtgcaacg tcgttcgacc ggggccgtcg ccgcgcgaaa tccaggcctt gaagggccgg
     1321 tccatgatta cagccggcgc ctgaccgaca gccgtgccga cttgcgcaag caacgcggcc
     1381 ccgcccgccg ctgcggccgc gctgtcgcgt agcaagctgc gtcgcgagat cgtccgtttc
     1441 ttgcccatgt cgcttctcgg ctggccaaaa gtggattgtc gatcttacgc cgcgagcctg
     1501 accgcgcacc cgtcacgcgc ggcttgccaa ggggtacccc ctgagctagt gttttcacta
     1561 ccagttcggt tgcatcaatc caacgatccg gggggggtcc catgaacaag cacccgcaag
     1621 aattgcggtt ttcgccgctc gcgcgagcca tcagccatgt cttcgccggc gtctccacgt
     1681 tgttgtttgt cggctacgcg acggcgcagc agccgccggc ggccgacgag cccgaagaga
     1741 tcgtcgtcac gggctcgcgc attcagcgca cgtcgggctt cgcgacggca gtgcccgtca
     1801 cggccgtcac tcgcgacgac ctgacgaatt tccaaccggg gcgcacgatg gcggatcagc
     1861 tcgatcagct gccgcaattc ttcgcgacgc agagcgcgca gcgcggcggc ggtgcgctat
     1921 tcggcagcgc cggtatcagc acgctgaacc tgcgcgcgat gggaccgcaa cgaacgctcg
     1981 tgctcgtcga cggtattcgc gtcgcgcctg ccgaccgcga cggcagcgtg cacatcgaca
     2041 acattccgtc ggcccttctg tcgcaggtcg aggtcgtcac gggcggcgcc tccgcggcct
     2101 acggtgccga cgcgctcgcg ggtgtcgtga atttccgcat caatcgccag ttcgaaggct
     2161 tggatttcaa cgtcggcacc ggtcgcaccg cagccggttt cggcgatcaa gtcgacgccg
     2221 cagccgcgta cggcacgccg ttcggcgaca agtggcact
//
LOCUS       DATLEE010000111         2861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_121285, whole genome shotgun sequence.
ACCESSION   DATLEE010000111 DATLEE010000000
VERSION     DATLEE010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121285"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VM692_02425"
     CDS             complement(<1..472)
                     /locus_tag="VM692_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HUQ51048.1"
                     /translation="MRNQRYDVPPLDPLLAFEAAARNLSFTKAAAELFLTQSAVSRQI
                     QQLEDHLKVKLFERRARTLLLTESGQILYRATQEALETLQDGAHRVRGAGTMDAVVVA
                     TTPGFATLWLIPHLAGFTRGHPHVDVRISATNEMIDDLERAGVDLAVRYVPERSV"
     gene            613..768
                     /locus_tag="VM692_02430"
     CDS             613..768
                     /locus_tag="VM692_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51049.1"
                     /translation="MKTRKDRGLRLAPPADVLARSRELFGDSLPELLLVTAVVVAVVS
                     AVLTLNG"
     gene            800..1138
                     /locus_tag="VM692_02435"
     CDS             800..1138
                     /locus_tag="VM692_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUQ51050.1"
                     /translation="MERNDQGPVTVTDADFAKTVLEAPRPVLVDFGAEWCGPCVAVEP
                     VIRSLALEYADRLTVATVDVDADPQLAARYGVRSLPTVMLLEEGQIRRVLVGARSGRE
                     YRAAIDGLLH"
     gene            1190..1639
                     /locus_tag="VM692_02440"
     CDS             1190..1639
                     /locus_tag="VM692_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUQ51051.1"
                     /translation="MSEHRATIDWRRSSADFTYVSYNRAHDWRFTAATVAASATKEYR
                     GDESRVNPEEALVAALSSCHMLTFLAVAAKRKLSLDSYRDEAVGVLEKNSEGKLAITR
                     VTLRPVIVWSAGATVSADELAKMHHDAHAGCFIANSVTTEVTVEPQP"
     gene            complement(1680..>2861)
                     /locus_tag="VM692_02445"
     CDS             complement(1680..>2861)
                     /locus_tag="VM692_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUQ51052.1"
                     /translation="FNHVLALDAAARTVEVEGLTTYETVVRHCLAQGFLPLVAPELKH
                     ITVGGATVGIGIESTCYRYGFVHDGLREADVLLPGGTIVTARADNEHADLFAALPNSY
                     GTLGYILRAKIALHPASPYVHLHVQKFTDIAAYLDAMRVATESPDLQFVEGLFFEDGR
                     YYLMTGRFVERAPEVDDIVRKHVFYRLVETRRDIYLKTFDYIFRYDPDWFWNVPETGF
                     FNVFRRHAPESWRNSAFYTKYVALKGKALGAVGVRDERMEPLIQDWEVPWRHGEELIR
                     FATENVDLAGRPWAAVPIKAPKQPTLYPIKPNELYFNLGCYCQVQRPAGMEPYHYTKI
                     LDRKCFELGGIKMLYSSSFLSEAEFDALYNGAAYRQLKAKYDAAGNAQTLYKKVALAP
                     R"
BASE COUNT          512 a          912 c          941 g          496 t
ORIGIN      
        1 ccacgctgcg ctccggtaca tagcgcaccg cgagatcgac gccggcgcgc tcgaggtcgt
       61 cgatcatctc gttggtcgcc gagatgcgca cgtcgacgtg cgggtggccg cgcgtgaagc
      121 ccgcgagatg cggtatcaac cagagcgtcg cgaagcccgg cgtcgtcgcg accacgaccg
      181 cgtccatcgt gccagcaccg cgcacgcgat gcgcgccgtc ctgcagcgtc tcgagcgctt
      241 cctgcgtcgc gcgataaagg atttggccgc tctcggtcaa cagcagcgtg cgggcgcgac
      301 gttcgaacag ctttaccttg aggtggtctt cgagctgttg gatctgccgg ctcacggccg
      361 actgcgtcaa gaacagctcg gccgcggcct tcgtgaacga caggttgcgg gcggccgcct
      421 cgaaggccag cagagggtcg agcggcggta cgtcgtagcg ctgattacgc atgcaaaaaa
      481 cgcatcgatt cgatgcggaa agaccgtttg tgacgcctcg attcgagccc cattatgcct
      541 gccgtacgca gagaaacgaa tggaaacgcg gttgcacatg cataccgaat tttcatggta
      601 acaggaggca atatgaagac tcgcaaagac cgtggcctaa ggctcgcgcc gccggccgac
      661 gtgctggccc gctcgcggga gctgttcggc gactcactgc cggagctctt gctggtgacc
      721 gccgtggtgg ttgccgtggt cagcgccgtg ttgacgctga acggctgacg caggacaagg
      781 actagctaag gagaggtcaa tggaacggaa tgatcagggg ccggttacgg tcaccgacgc
      841 cgatttcgcc aagaccgtgc tcgaagcgcc gcgcccagtg ctcgtcgact tcggcgccga
      901 gtggtgcggc ccgtgcgtgg ccgtggagcc ggtgatccgc agcttggcct tggaatatgc
      961 ggatcggctg acggtcgcca ccgtcgacgt cgacgccgat ccgcagctcg ccgcacgcta
     1021 cggcgtgcgg tcgttgccga cggtcatgtt gctcgaggaa gggcagatcc gccgcgtgct
     1081 cgtaggcgcg cgctcgggcc gggaataccg cgccgcgatc gacggcttgc tgcactgaag
     1141 cgctcgacga gcccggtaga atgcaaggct agcactcgcg gagaagacca tgtccgaaca
     1201 ccgagccacg atcgactggc ggcgcagcag cgccgatttc acctacgtga gctacaaccg
     1261 tgcgcacgac tggcgcttca cggccgcgac ggtcgccgcc tcggccacta aggaataccg
     1321 gggcgacgag agccgcgtca atcccgagga ggcgctcgtc gccgcattgt cctcgtgcca
     1381 tatgctgacg tttctcgccg ttgccgcgaa gcgcaaactg tcgctcgact cctatcgcga
     1441 cgaagccgtc ggcgtgctcg agaagaactc ggaagggaag ctcgcgatca cgcgcgtcac
     1501 gctgcgcccg gtaatcgtct ggtccgcggg cgccacggtc tcggccgacg agctggccaa
     1561 gatgcaccac gacgcgcacg ccggctgttt cattgcgaat tccgtcacga ccgaagtcac
     1621 cgtcgagccg cagccgtaat cgccgtcacg cccagcgctg cgggccggtc gcggcgccgt
     1681 cagcgcgggg ccagcgcgac cttcttgtat agcgtctgcg cgttgcccgc cgcgtcgtac
     1741 ttcgctttga gctgacggta ggccgccccg ttgtacagcg cgtcgaactc cgcctcgctc
     1801 aagaacgacg acgagtagag catcttgatg ccgccaagct cgaagcactt gcggtcgagg
     1861 atcttcgtgt agtgatacgg ctccataccg gccggtcgct gcacttggca gtaacagccc
     1921 aagttgaagt agagctcgtt cggcttgatg gggtagagcg tcggctgctt cggcgctttg
     1981 atcggcacgg ctgcccaggg tcggcccgcg aggtcgacgt tctcggtcgc gaaccggatc
     2041 agctcttcgc cgtgccgcca cggcacttcc cagtcctgga tcaacggttc catgcgctcg
     2101 tcgcgaacgc cgacagcgcc gagcgctttg cccttcaatg cgacgtattt cgtgtagaac
     2161 gcggagttgc gccaactctc cggcgcatgc cggcgaaaca cgttgaagaa gccggtctca
     2221 ggcacgttcc agaaccagtc cgggtcgtag cgaaagatgt agtcgaacgt cttcaggtaa
     2281 atgtcgcgcc gcgtttcgac gagccggtag aaaacgtgct tgcgcacgat gtcgtcgacc
     2341 tcgggcgccc gttcgacgaa gcggccggtc atcaggtagt agcgcccgtc ctcgaaaaac
     2401 agaccttcga cgaactgcaa gtccggcgat tccgtcgcga cgcgcatcgc atcgagatac
     2461 gccgcgatat ccgtgaactt ctgcacgtgc aggtgaacgt acgggctggc cgggtgtaac
     2521 gcgatcttgg cgcgcaagat gtagccgagc gtgccgtacg agttcggcag cgcagcgaag
     2581 agatcggcgt gctcgttgtc ggcgcgcgcc gtgacgatcg tgccgcccgg cagcagaacg
     2641 tcggcttcgc gcagtccgtc gtgcacgaaa ccgtagcgat agcacgtcga ctcgatgccg
     2701 atgccgacgg tcgcgccgcc gacggtgatg tgcttgagct cgggtgccac gagcggcaag
     2761 aagccctgcg ccaagcaatg gcgcacgacg gtctcgtagg ttgttagccc ctcgacctcg
     2821 accgtgcgcg cggcggcgtc gagcgcaagc acgtgattga a
//
LOCUS       DATLEE010000112         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9058205, whole genome shotgun sequence.
ACCESSION   DATLEE010000112 DATLEE010000000
VERSION     DATLEE010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9058205"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1878
                     /locus_tag="VM692_02450"
     CDS             148..1878
                     /locus_tag="VM692_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51053.1"
                     /translation="MVLRRIIGSSVALGLALTIGTAAAQFGPPGPPPYTPAPDAKDLK
                     SVLFNWTWHMGMLRGVEEHELAVSLDYQGTGTMQVNGQPCTLTKYRSSINYQAPGQRI
                     QYTCTRPNGQTVSNIEVVSGTFAWNEDTPGAELVAGQGKATPMPRTVQERLIRLWASP
                     QGAAKAALAGAGVDLLAMNRNPGGLLQEGQTKIGNTSVAWEGSKPVVTFPIPGVSGAT
                     ATATLDNRFMAERVVVKQGSTTTEFEYSNYDDWNNPLNKIEAYYAGKMVERRNGTVVR
                     DLTTTETETGSVYVVMPVPASVGKAKGFPLLPTDRTAVLASTAETPRMDNGKPDLTGN
                     WSAGGMNWRYGNRRCAPTQLEGCTPAWNQTLDFEFEAPSRFGPNRPLYRPEHWDKVIA
                     LDQWTNKEDPVMTCQPLGIPRQGPPRRIIQSADDVVFFYSQYGDGGGGQAEFRIIPTD
                     GRERNARDGLETKYYGYTIGKWEGDTLVLDSTSFNDYTWLARGGFFHSDQMHVIEKLT
                     RKGDEMLYEVIVEDPVVLLEPWVMTPRIMKLASNNVPEFPGFPPGLGVTERGHCEVYE
                     LEDISTQIRH"
     gene            complement(1944..>2195)
                     /locus_tag="VM692_02455"
     CDS             complement(1944..>2195)
                     /locus_tag="VM692_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HUQ51054.1"
                     /translation="KAWDLDLAKRRDVATLYERVHAAAASVCRAETDKLYRDTRIRAP
                     SGWRAQCVQDAVDATVRRVGNPALAALHTQAPRVAADVR"
BASE COUNT          419 a          732 c          721 g          323 t
ORIGIN      
        1 aaagccggcg atccggtcac gatcacatac ttcccggcgc gtagcggcaa gccgctcggc
       61 ttcatccgct cgatcgtctt ggccgacaag cgcgagatcg agatctcgaa cggcgccagc
      121 gccgactaga cccagcgagg caacgacatg gttttacgac gcatcatcgg ctcgagcgtc
      181 gcgctcgggc tcgcgctcac gattggcacg gccgcagcac aattcgggcc gcccgggcct
      241 ccgccgtaca caccggcgcc cgacgcgaaa gacttgaagt cggtcctgtt caactggacc
      301 tggcacatgg gcatgctgcg cggcgtcgaa gagcacgagc tcgccgtgtc gctcgactat
      361 cagggcacgg gcacgatgca ggtcaacggc cagccgtgca cgctgacgaa gtaccgctcg
      421 agcatcaatt accaggcccc gggtcagcgc atccagtaca cgtgcacgcg gccgaacggc
      481 caaacggtgt cgaacatcga ggtcgtcagc ggcacgttcg cgtggaacga agacactccc
      541 ggcgccgagc tcgtggccgg gcagggcaaa gcgacgccga tgccgcgcac cgtgcaagag
      601 cggctgatcc gcttgtgggc gagcccgcaa ggtgcagcca aggccgcgct tgcgggcgcg
      661 ggcgtcgatc tgctggcgat gaatcgcaat ccgggcggcc tgctgcaaga aggccaaacc
      721 aagatcggca acacgagcgt cgcatgggaa ggctcgaagc ccgtcgtgac gttcccgatc
      781 ccgggcgtct cgggcgcgac ggcgacggcc acgctcgaca accgcttcat ggccgagcgc
      841 gtcgtcgtca agcaaggctc gacgacgacc gagttcgaat acagcaacta tgacgactgg
      901 aacaacccgc tgaacaagat cgaggcgtac tacgccggca agatggtcga gcggcgcaac
      961 ggcaccgtcg tacgcgatct gacgaccacc gagaccgaga cgggcagcgt ctatgtcgtc
     1021 atgcccgtgc ccgcgagcgt cggcaaagca aagggcttcc cgttgctgcc gaccgaccgc
     1081 acggcagtgc tggcgtcgac ggctgaaacg ccgcgcatgg acaacggtaa gccggatctc
     1141 acgggcaact ggagtgccgg tggcatgaac tggcgctacg gcaatcgccg ctgcgcgccg
     1201 acgcagctcg aaggctgcac gccggcgtgg aaccagacgc tcgatttcga gttcgaggcg
     1261 ccgtcgcgct tcggcccgaa tcgcccgctc tacaggcccg agcactggga caaggtcatc
     1321 gcgctcgacc agtggacgaa caaagaagat cccgtcatga cctgccagcc gctcggcatc
     1381 ccgcggcaag ggccgccgcg ccgcatcatc cagtcggccg acgacgtcgt gttcttctac
     1441 agccagtacg gcgatggcgg cggcgggcaa gcggagttcc gcatcattcc gaccgacggc
     1501 cgcgagcgta acgcacgcga cgggctcgaa accaagtact acggttacac gatcggcaag
     1561 tgggagggcg acacgctcgt gctcgactca acgtcgttca acgactacac gtggctcgcg
     1621 cgcggcggct tcttccactc ggaccagatg cacgtcatag agaagctcac gcgcaagggc
     1681 gacgagatgc tgtacgaagt gatcgtcgag gatcccgtcg tgctgctcga gccgtgggtc
     1741 atgacgccga ggatcatgaa gctcgcttcg aacaacgtgc ccgagtttcc ggggttcccg
     1801 ccggggctcg gcgtgaccga acgcggtcac tgcgaagttt acgagctcga ggacatctcg
     1861 acgcagatcc gccactaggc gccccgtcga cgaatcgcaa tgccgctcgg ggcaacccga
     1921 gcggcatttt tttcgcgcgc gagctagcgg acgtcggctg cgacgcgcgg tgcttgcgtg
     1981 tgcaacgcgg cgagcgccgg gttgccgacg cggcgaaccg tcgcgtcgac ggcatcttgc
     2041 acgcactgcg ctcgccagcc gctcggcgcg cgaatgcgcg tgtcgcgata cagcttgtcg
     2101 gtctccgcgc ggcagacact ggcggccgcg gcgtgcacgc gctcgtagag cgttgccacg
     2161 tcgcgccgct tcgccaagtc gaggtcccaa gcttt
//
LOCUS       DATLEE010000113         3338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9058506, whole genome shotgun sequence.
ACCESSION   DATLEE010000113 DATLEE010000000
VERSION     DATLEE010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9058506"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..2445
                     /locus_tag="VM692_02460"
     CDS             88..2445
                     /locus_tag="VM692_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ51055.1"
                     /translation="MTNELVKSSVVARLWPAIASIAHARRSLSGRLMLVMLTTTAIAL
                     LAAGASLLLIDLRNDRAAWAEDLGTEAAILSLAVQPALSFQDVQGAQRNLNSLQARAS
                     IQAATLYTPDGALFAAYSRSPETAPPARAPSLAPGTHIDGERVEVVSRIVQSGETLGT
                     IYLRAQYDFNGRVRAYISVLSAIMLIGLVAALLASSWLQRVVSEPMESMATAARQIVQ
                     TRDYSFRAAKTTDDEIGVVIEAFNKMLDEVESRSRALETSEKLYRAIGESIDYGVWVC
                     DAEGRNIYASDSLLKLTGMTAEEAAELGWARVLHPDDLEDTVAAWKECVRTGHIWYRE
                     HRMLGVDGKYHAILAHGVPIRDANGRVQRWAGINLDISRLKNTEHALREADRRKDEFL
                     ATLAHELRNPLAPIRNAVRILDSDAADATQRKWGREVIARQVHRMSLLLDDLLDVSRI
                     TRGQFELKKDYVELRPLVGVAVETARPLIDAKGHDLVVTLPDEPIMLEADPLRLSQVI
                     GNLLTNAAKYTDPRGRIELRAQVEKGELVITVRDNGIGLENAVLPGLFTMFSQVNSAI
                     DRAEGGLGIGLALVKGLVGLHGGQVEARSDGPGRGSEFIVRLPHEVLAPASVLADAAT
                     VTRKSGAAKRARVLVVDDNRDAAESLGMVLQFAGYEVTTAFNGRQALEVGALARPLAA
                     IIDIGMPGMSGHEVARRMRLEAWGRNAALIALTGWGQDSDKQASRASGFDAHLTKPVD
                     PDELGNVLTSLLSRKANGTGAAEVVDPARAFGDRSASGGATPPSE"
     gene            complement(2463..2819)
                     /locus_tag="VM692_02465"
     CDS             complement(2463..2819)
                     /locus_tag="VM692_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51056.1"
                     /translation="MRRRLPHEPAHLIEQLTIWIEWRYADSHESLVLTSAPTSVPLAV
                     NRWLPGAVWRLAWQGDGSAVLYGISPGSAPNKYFVARHDGVASRREGLFERVGDEHWV
                     HLDGDSLRSLRAALAA"
     gene            2967..3305
                     /locus_tag="VM692_02470"
     CDS             2967..3305
                     /locus_tag="VM692_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51057.1"
                     /translation="MAFHDGWLKLAHAFGALKARMRSKQALDLAWPSSALVSPVMKHG
                     PGGSELSFLLSPVNDDTFAALLAAKRDRVPVRLRSLRRPLALTITEVERVASGARVAG
                     RVAYRTRAEL"
BASE COUNT          589 a         1090 c         1128 g          531 t
ORIGIN      
        1 accaaaagtt tcgccattct atcaaaaaaa atatattttt gagaaggcag tggaccggat
       61 ttccactctt ggggcaagat gccggctatg actaacgagc tcgtcaagag cagcgtcgtc
      121 gcgcgcctgt ggccggcgat cgcttcgatc gcgcacgcgc gtcgcagctt gagcggcagg
      181 ctcatgctcg tgatgttgac gaccacggcg attgcgctac tggcggcggg cgcgagcttg
      241 ttgctgatcg atttgcgcaa cgaccgcgcg gcctgggccg aggatctcgg cacggaagcc
      301 gcgatcctgt cgctggccgt gcagcctgcg ttgtcgttcc aggacgtgca gggggcgcag
      361 cgcaacctga actcgctgca ggcgcgcgcc tccattcaag ccgcgacgct ttacacgccc
      421 gacggtgccc tgttcgcggc ctactcgagg tcacccgaga cggcgccgcc cgctcgagca
      481 ccctcgctgg cgcccggcac gcacatcgac ggtgagcgtg tcgaagtcgt gagccgcatc
      541 gttcagagcg gcgagacgct cggaacgatc tatctacgcg cgcaatacga cttcaacggc
      601 cgcgtgcgcg cctatatctc ggtgctgagc gccatcatgc tcatcgggct cgttgccgcg
      661 ctgttggcgt cgagctggct gcagcgcgtc gtcagcgagc ccatggaatc gatggcgacc
      721 gccgcgcgcc agatcgtgca gactcgcgat tactcgttcc gcgccgccaa gaccaccgac
      781 gacgagatcg gcgtagtcat cgaagcgttc aacaagatgc tcgacgaggt cgagtcgcgc
      841 tcgcgcgcgc tcgagacgag cgagaagctc tatcgcgcca tcggcgagtc gatcgactac
      901 ggtgtctggg tctgcgacgc ggaaggtcgc aacatctacg cgagcgactc gctgctcaag
      961 ctcaccggca tgacggccga ggaagcggcg gagctcggct gggcaagagt cctgcacccc
     1021 gacgacttgg aggacaccgt cgcggcttgg aaggagtgcg tgcgcaccgg ccacatatgg
     1081 taccgagagc accgcatgct gggcgtcgac ggcaagtacc acgcgatcct cgcgcacggc
     1141 gtgccgattc gcgatgcgaa cggccgagtg cagcggtggg ccggcatcaa tctcgacatc
     1201 tcgcgcctga agaacaccga gcacgcgctg cgcgaggccg atcgacgcaa ggacgagttt
     1261 ctcgcgacgc tggcccacga gctgcgcaac ccgttggccc cgatccgcaa cgccgtccgt
     1321 attctcgatt cggacgccgc ggacgccacg cagcgcaagt ggggccgcga agtcatcgcg
     1381 cgccaggtgc atcgcatgtc gctgctgctc gacgacctgc tcgacgtctc gcgcatcacg
     1441 cgcggccagt tcgagctcaa gaaggattac gttgagctga ggccgctcgt cggtgtggcc
     1501 gtcgagaccg cgcggccgtt gatcgacgcc aaggggcacg atctcgtcgt gaccttgcca
     1561 gacgagccga tcatgctcga agcagatccg ctgcggctgt cgcaagtcat cggcaatttg
     1621 ctgaccaacg cagccaaata caccgatccc cggggacgca tcgagctacg cgcgcaggtc
     1681 gaaaaaggcg agctcgtgat tacggtgcgc gacaacggta tcggcctcga gaacgcggtg
     1741 ctgccggggc tgttcacgat gttctcgcag gtcaactcgg cgatcgatcg cgccgaaggc
     1801 gggctcggca tcggtcttgc gctcgtcaag ggcttggtgg gattgcacgg ggggcaagtc
     1861 gaagcgcgca gcgacgggcc cggccgcggc agcgagttca tcgtgcgcct gcctcacgaa
     1921 gtactcgcgc ccgcgagcgt gctcgccgac gcagcaacgg tcacgcgcaa gagcggtgcc
     1981 gccaaacggg cgcgcgtact cgtcgtcgac gacaaccgcg acgcggccga gtcgctgggc
     2041 atggtccttc agttcgccgg ttacgaggtg acgacggcgt tcaacgggcg gcaagcgctc
     2101 gaggtcgggg cgctcgcgcg accgctcgcg gccatcatcg acatcggcat gccgggcatg
     2161 agcggtcatg aagtcgcgcg gcgcatgcgc ctcgaggcgt ggggccgcaa cgcagcgctg
     2221 attgcgctga cgggctgggg gcaggacagc gacaagcaag catcgcgagc gtcggggttc
     2281 gacgcgcatc tcacgaagcc tgtggatccc gacgagctcg gcaacgtgct gacgtcactc
     2341 ctgagccgca aagcgaacgg tacgggcgcc gccgaggtcg tcgatcccgc gcgcgcgttc
     2401 ggcgaccggt cggcgagcgg cggcgcaacg ccgccgtcgg agtaacgacg cggaggctgc
     2461 cgctaggccg cgagcgcggc gcgcagcgac ctcaggctgt cgccgtcgag atgcacccaa
     2521 tgttcgtcgc ccacacgctc gaacaaccct tcgcgccgcg aggcgacgcc gtcgtgacgc
     2581 gcgacgaaat acttgttcgg cgcgctgccg gggcttatgc cgtacagcac ggcggagccg
     2641 tccccttgcc acgcaaggcg ccaaaccgca ccgggtagcc agcgattcac cgcgagcgga
     2701 accgaggtcg gcgccgacgt caaaacgagg ctttcgtgag aatcggcgta acgccactcg
     2761 atccagatcg tgagctgctc gatgaggtgc gcgggctcgt gaggcagtct tcgccgcatc
     2821 tcccaatcct ccttgtagac gaattaaagg cgtgtcggct gggcctccaa agtctcgcat
     2881 ctccgagggg taaacgggaa cccttttgcg cgttcgagcg tgtaaggaac tgaggtgcag
     2941 gatctcaggc acgacgagag agaccaatgg cattccacga cggatggctc aaattggcgc
     3001 acgcgttcgg cgcgctgaag gcccgcatgc gctccaaaca ggcgctcgac cttgcctggc
     3061 ccagctcggc cctcgtgtcg cccgtcatga aacacggtcc cggaggatcg gagctttcgt
     3121 tcttgttgtc cccagtcaac gatgacacgt tcgcggcact cttagccgcg aagcgcgacc
     3181 gggttcccgt gcgcctgcgc tcgctgcggc ggccgctggc gctgacgatc acggaggtcg
     3241 agcgcgtcgc gagcggcgcc agggtggccg ggcgcgtcgc ttatcgaacg cgcgcggagc
     3301 tctagctcga gggcgcgtcg cgcacgcgcg gtggcggc
//
LOCUS       DATLEE010000114         2099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5341069, whole genome shotgun sequence.
ACCESSION   DATLEE010000114 DATLEE010000000
VERSION     DATLEE010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5341069"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VM692_02475"
     CDS             <1..663
                     /locus_tag="VM692_02475"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HUQ51058.1"
                     /translation="LKMPGASRAQSDRSLACLAALLASAAITAANAAPDSGALIARLA
                     RQPPATTAFTEVRFSALLAEPLVVSGMLDYERDGVLNRRVETPYRETTTISGETVRVE
                     RDGDKPRTFALRRAPELRGLLTGMIGLLTGDGSFIGAQFKVSTAGDDESWRIELEPTD
                     SRLRERLSAIRVVGAGDEPHCFVILDTQGGASVMLLGETASAPLPQPIVLAGLLGHCA
                     TG"
     gene            660..>2099
                     /locus_tag="VM692_02480"
     CDS             660..>2099
                     /locus_tag="VM692_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51059.1"
                     /translation="MKRSAAALLLLLAWAASLAVLGLLVERRLDVGSDLRLFLPTPQT
                     PEQRLLLEAIGEGPASRLLVVTLEGAPAESLAAASSALVDALGGDERFRFIANGDVGL
                     ESLPQTLLPYRYLLSPDDATRAFDRATLRAALEARARDLASPGGFALESLIARDPTLL
                     TLALVERWQPAREPRRELDVWFDETGTRALLVAETRAPAFDPAGQRAALAALDAAFAS
                     AGAPPGTTLTASGAGSFSVLMEARTRSAAENLARFATIGMLLVLLVAYRRIGALVLSA
                     LPLASAAVAGLAAVSALFGSVHGITLAFGFTLLGVAQDYPLHLLSHRRPGVEPSRVAA
                     GLWPTLATGVASTCVAYLTFWFSGVTGLEQLACLTVTGLAVAGLTTRYLLPHLMTQGP
                     RDYGDSPSLQRLWRAIASLPRPWWAAVVLVGVCVGVVSFAPQPFWENDLSALTPVPSD
                     LLERDRALRAELGTADTRHLLVVEAASANA"
BASE COUNT          254 a          770 c          740 g          335 t
ORIGIN      
        1 ctaaagatgc ccggcgcctc acgcgcacag agcgatcgct cgctggcgtg cctcgcagcg
       61 ctgctcgcct cggccgccat cacggcggcg aatgcggcgc ccgatagcgg ggcgctgatc
      121 gcacggctcg cgcgccaacc acccgcaacc acggcgttca cggaagtgcg tttttcggcg
      181 ctgctcgccg agccgctcgt cgtcagcggc atgctcgatt acgagcgcga cggagtcttg
      241 aaccgccgcg tcgagactcc gtatcgcgaa acgacgacga tcagcggcga gaccgtgcgc
      301 gtcgaacgcg acggcgacaa gccgcgcacg ttcgcgctgc gccgcgcgcc cgaattgcgc
      361 ggcttgttga cgggcatgat cggcttgctg acgggtgacg gctcgttcat cggcgcgcag
      421 ttcaaggtgt cgacggccgg cgacgacgag agctggcgaa tcgagctgga gccgacagac
      481 agccggttgc gcgagcgtct gagcgcgatc cgcgtcgtcg gcgcgggcga cgagccgcac
      541 tgcttcgtca tcctcgacac gcaaggcggc gccagcgtga tgttgctcgg cgagaccgcg
      601 agcgcgccgc tgccgcagcc gatcgtgctc gcgggattgc tcggccactg tgccacggga
      661 tgaagcgcag cgccgcggcc ctgttgctgc ttctcgcgtg ggccgcaagt ctcgcggtgc
      721 taggcctgct cgtcgaacgc cgcctcgacg tcggctcgga cctgcggctg ttcctgccga
      781 cgccgcaaac gccggaacag cggctgctgc tcgaagccat cggcgaaggc cccgcctcgc
      841 ggctgctcgt cgtcacgctc gaaggcgcac cggccgagtc gctcgccgcg gcgtcgagcg
      901 cgctcgtcga tgcgctcggc ggcgacgagc gattccgttt catcgccaac ggcgacgtcg
      961 gcctcgagtc gctgccgcag acgctgctgc cgtatcgcta cctgctgtcg ccggacgacg
     1021 cgacgcgcgc cttcgatcgt gcgacgctgc gcgccgcgct cgaggcgcgc gcgcgcgacc
     1081 tcgcctcgcc gggcggcttc gcgctcgagt cgttgatcgc gcgcgatccg acgcttctga
     1141 cgctggcctt ggtcgagcgc tggcagcccg cacgcgagcc gcgtcgtgag ctcgacgtct
     1201 ggttcgacga gaccggcacg cgcgccctgc tcgtcgccga gactcgcgca ccggctttcg
     1261 atccggccgg gcagcgggcg gcgctcgccg cgctcgacgc tgcgttcgcg agcgccggcg
     1321 cgccgcccgg cacgactctg acggcgagcg gcgccggcag cttctcggtc ctcatggagg
     1381 cgcggacgcg cagcgcggcc gagaatctcg cgcgcttcgc gacgatcggc atgctgctcg
     1441 tgcttctcgt cgcgtatcgc cgcatcggcg ctctggtgct cagcgcgttg ccgctcgcga
     1501 gcgccgccgt cgccggcctc gcggcggtca gcgcgttgtt cgggtccgtg cacggcatca
     1561 cgctcgcgtt cggcttcacg ctgctcggcg tcgcgcagga ctatccgctg cacctgctga
     1621 gtcaccgccg gcccggcgtc gagccgagcc gcgtcgcggc cgggctctgg cccacgctcg
     1681 cgaccggcgt cgcgagcacc tgcgtcgcgt atctgacgtt ttggttcagc ggcgtgacgg
     1741 gcctcgagca gctcgcgtgc ttgacggtca cgggcctcgc cgtcgcaggc ctgaccacgc
     1801 gctatctgct gccgcacctc atgacgcaag ggccgcgcga ctacggcgac tcgccgtcgc
     1861 tgcagcggct ctggcgagcg atcgcgtcgt tgccgcggcc gtggtgggcg gccgtggtac
     1921 tcgtcggcgt ttgcgtcggc gttgtctcat tcgcgccgca gccgttctgg gaaaacgatc
     1981 tcagcgcgct gacgccggtg ccgtccgact tgctcgagcg cgatcgcgcg ctgcgcgccg
     2041 agctcggaac ggccgacacg cgccatctgc tcgtcgtcga agccgcgtcg gccaatgcg
//
LOCUS       DATLEE010000115         2768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_870466, whole genome shotgun sequence.
ACCESSION   DATLEE010000115 DATLEE010000000
VERSION     DATLEE010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870466"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VM692_02485"
     CDS             <1..292
                     /locus_tag="VM692_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51060.1"
                     /translation="HLYTLEAALASVDSRIAANYRAQENIKQELTSIAAKMIAADTTT
                     EQRLTMVTRTAELGRRHGELNAEIQQLERDRAYAEREALDYREALAGTFTPP"
     gene            complement(312..1313)
                     /locus_tag="VM692_02490"
     CDS             complement(312..1313)
                     /locus_tag="VM692_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HUQ51061.1"
                     /translation="MTSRPRLRAALAVALAVLGLHACSRDSLPNPYRTITGWMKGPPN
                     RVFGSMSMVDVAPDGSLWIAERCGANDCLPHGDLDPILHVAANGEWLGAFGAGLFAWP
                     HGVYVDAAGNIWVTDARGGDSVGHQVIKFAPDGRELLRLGTSAHAGDGPTHFNGPTDV
                     VVAPNGDVFVSDGHEPKSNNRIVKFAADGRYLKDWGGSGSAPGQFLVPHALALDSQGR
                     LFVADRDNNRIQIFDQDGKFLDAWTQFGRPSGIHIAADDTLYVSDNQSNDERHPGWPR
                     GLYVGSAKDGSVSAFVPDPEFDPKQTQETGAHGLAADAAGTIYGAEVYSQSVKKYVR"
     gene            1455..1718
                     /locus_tag="VM692_02495"
     CDS             1455..1718
                     /locus_tag="VM692_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51062.1"
                     /translation="MSDAPHTSAYQREDITEGKAGVPLSLTLNIVNVSNQCAPILTAM
                     VYIWHRDKDGLPRPRVAGDLAARVLEHGDECCLRDVAVCRQRR"
     gene            1935..>2768
                     /locus_tag="VM692_02500"
     CDS             1935..>2768
                     /locus_tag="VM692_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009024003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HUQ51063.1"
                     /translation="MRVLRRVPVAAPIALALSPLAASIALAQDQLEEIIVTARLRAES
                     YTETPAAITAFTAAEIESAGIDTPHDFIALTPNMTVVQTQNPGNSFITIRGVSQARNS
                     DMPVAVVVDGVLMTNPAQFNQELFDIEQIEVLKGPQGALYGRNAIGGAVSIVTKTPTD
                     DFEAKLRVGADSGPGWLAQGTVSGPLGDGDAWKYRASLSLSDTDGFRENEFLHDKADP
                     YKDLSARVRFMWEPNDKVTGDFRYARSDIDTTALYFVINDDQRTLGSFVGVPNPNVLS
                     GV"
BASE COUNT          506 a          896 c          912 g          454 t
ORIGIN      
        1 ccacctgtac acgctcgagg ccgcgctcgc gagcgtcgat tcgcgcattg cggcgaacta
       61 ccgcgcgcaa gaaaacatca agcaagagct gacgtcgatc gcggccaaga tgatcgcggc
      121 cgacacgacg acggagcagc gcttgacgat ggtcacgcgg accgcggagt tggggcggcg
      181 tcacggcgag ctgaacgccg agatccagca gctcgagcgc gaccgcgcgt atgccgagcg
      241 cgaagcgctc gactatcgcg aggccttggc cggaaccttc acgcctccct gaagaccggc
      301 acctcggttg ttcagcgcac gtacttcttg acgctctgcg agtagacctc cgcgccgtag
      361 atcgtgccgg ccgcgtcggc cgccaaaccg tgagcgccgg tctcctgcgt ttgcttcgga
      421 tcgaactccg ggtccggaac gaacgcgctg accgagccgt ccttcgcact gccgacgtag
      481 agaccgcgcg gccagcccgg atgccgctcg tcattcgatt gattgtccga gacgtacagc
      541 gtgtcgtcgg cggcgatgtg gatgccgctc ggtcggccga actgcgtcca cgcgtcgagg
      601 aacttgccgt cctggtcgaa gatctggatg cgattgttgt cacgatcggc gacgaacaac
      661 cggccctgcg agtcgagcgc gagcgcgtgc ggcacgagga actgtcctgg cgcgctgccg
      721 gagccgcccc agtccttgag atagcggccg tcggcggcga acttcacgat gcggttgttc
      781 gacttcggct cgtggccgtc gctgacgaat acgtcgccgt tcggcgcgac gacgacgtcg
      841 gtcgggccgt tgaaatgcgt cgggccgtcg ccggcgtgtg ccgacgtgcc gagccgcagc
      901 agctcgcggc cgtccggcgc gaacttgatg acttgatggc cgacgctgtc gccgccgcgc
      961 gcatcggtaa cccaaatgtt gccggcggcg tcgacgtaga cgccgtgcgg ccacgcaaat
     1021 agccctgctc cgaacgcgcc cagccattcg ccgttggcgg cgacgtgcag gatcggatcg
     1081 aggtcgccgt gcggcaagca atcgttcgcg ccgcagcgct cggcgatcca tagcgagccg
     1141 tcgggtgcca cgtcgaccat gctcatggag ccgaagacgc gattcggcgg acccttcatc
     1201 cagcctgtga tcgtccgata cgggttcggt agagagtccc gcgagcaagc gtgcagaccg
     1261 agcacggcga gcgcgaccgc gagagcggcg cgcaaacggg gtcgcgaagt catggcgcga
     1321 gtctgcgcgc ggctcgcatg gcgttcaagc gggcggcagc gcgcgcgttt acagttgctt
     1381 acagtgcagt cggaagcgtt tggctaggct gctccgtgcc gtcgcacgag gagcttgagg
     1441 atagagacca cgccatgagc gatgcgccgc acacgtctgc ttatcagcgc gaggacatta
     1501 ccgagggcaa ggccggcgtg ccgctgagcc tgacgctgaa catcgtcaac gtcagcaatc
     1561 agtgcgcgcc gattctgacg gcgatggtct acatctggca tcgcgacaag gacggtctac
     1621 ctcggcctcg cgttgcaggc gacctcgcag ctcgcgttct cgaacacggt gacgagtgct
     1681 gtttacgcga cgtcgctgta tgccgccaaa ggcgataacc cgacgaagcc gaacagcgat
     1741 ggcatcttcg cggacggcat gacgtaccaa atgccgaccc tgacgccaat cgcgacgacg
     1801 cgtacgacgc cgagctgacg gtcggaattg cggcgtagtg tgttactgcc accaccacca
     1861 aacataacgg tgacgacgat gggcccggtg ctgtaaagtg cgccgacgca acactcactt
     1921 ctccaggggg aaccatgaga gtgctaagac gagtacccgt cgccgccccg atcgctctgg
     1981 cgttgtcgcc cctcgctgcc tcgatcgcgc tcgcgcaaga tcagcttgag gaaatcatcg
     2041 taaccgcccg tttgcgcgca gagtcttaca cggagacgcc ggcggccatc acggccttca
     2101 cggccgccga gatcgagtcc gcgggcatcg acacgccgca cgatttcatt gcgttgacgc
     2161 cgaacatgac ggtcgtgcag acgcaaaacc ccggcaactc gttcatcacg atccgcggtg
     2221 tgtcgcaggc gcgcaacagc gatatgccgg tcgccgtcgt cgtcgacggc gtgctgatga
     2281 cgaacccggc gcagttcaat caagagctgt tcgacatcga gcagatcgag gtgctgaagg
     2341 gaccgcaagg tgcgctctac ggccgcaacg ccatcggcgg cgcggtgtcg atcgtgacca
     2401 aaacgccgac cgacgacttc gaggcgaagc tgcgcgtcgg cgcggactcg ggcccgggtt
     2461 ggctcgcgca aggcacggtc agcggcccgc tcggcgatgg cgatgcctgg aagtaccgcg
     2521 cttcgctgtc gctctcggac acggacggtt tccgcgagaa cgagttcctg cacgacaagg
     2581 ccgacccata taaggacctg tcggcgcgcg tgcgtttcat gtgggagccg aacgacaagg
     2641 tgacgggcga cttccgttac gcgcgttccg acatcgacac gacggcgctg tacttcgtca
     2701 tcaacgacga tcaacggacg ctcggcagtt tcgtcggcgt gccgaacccg aacgtgttga
     2761 gcggcgtc
//
LOCUS       DATLEE010000116         5278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8262064, whole genome shotgun sequence.
ACCESSION   DATLEE010000116 DATLEE010000000
VERSION     DATLEE010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8262064"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..837
                     /locus_tag="VM692_02505"
     CDS             67..837
                     /locus_tag="VM692_02505"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HUQ51064.1"
                     /translation="MAVLVDIGNTRIKWALLERGRLTGRGSAVHRDSLDAAIAALDAA
                     LPAKPQIIASNVAGDVAAARLEALVAARPGASLELVSTSAERFGVRCAYADPSRLGVD
                     RWVAVLAAHHAQRARAACVLSAGTAVTFDAVDAAGLHLGGLIIPGASLLAAALDRHTS
                     NIGRTAPATAVPEGLELLGRNTDKAVGHGAWLAIAAALDRAVATIADALRTTPVVYLT
                     GGDADALRGWLATRVELRADLVLEGLQLFAGARAEAGR"
     gene            837..1661
                     /locus_tag="VM692_02510"
     CDS             837..1661
                     /locus_tag="VM692_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HUQ51065.1"
                     /translation="MRNFFLALVLANLGFAAWSAWFAAPQRSGRPADDGLPALTLVSE
                     VPADLRPSGAPLRGVVVEPADASESATASESASAERPTTAAATDTAAPSRAAPGAEPP
                     PSEQPLATAADRSTTAAARCASVGPFRELSQVATAAAALRAEGYQPQQRVAEGEIWIG
                     YWVYIGGIATEAAANEILAKVRDGGIADSQVIPNSDSGNLVSLGVFSEISGVSRRREQ
                     VRALGFEPQVVDRTRRATVYWIDVSLGPDQTLDYDALQTPGRISRLEQRDCEQSPG"
     gene            1774..2520
                     /locus_tag="VM692_02515"
     CDS             1774..2520
                     /locus_tag="VM692_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HUQ51066.1"
                     /translation="MRLVNSTTWTLALCLLGSALAFDAQAQDEQAARMRTALAAPARP
                     AEDKERDAVRKPIETVQFLGIKTGQTVIDVIAAGGWFTEVLSAAVGPSGKVLAQNPEF
                     FVSRPGFAEAEKQRNDRLGNVQPVHGELAANGVVAQADAAITALNMHDLYNGQNGEAA
                     AVAFAKGVYDALKPGGTFGVIDHVGIAGQNNASFHRMQLAQARDVLTKAGFRIEAESN
                     ILANPADDHTKGVRDMARHTDQFLIRARKP"
     gene            2627..2701
                     /locus_tag="VM692_02520"
     tRNA            2627..2701
                     /locus_tag="VM692_02520"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2659..2661,aa:Thr,seq:tgt)
     gene            2797..3006
                     /locus_tag="VM692_02525"
     CDS             2797..3006
                     /locus_tag="VM692_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51067.1"
                     /translation="MFTSTIILSGTVVIEGTTFRWWLTDGVAQQLTVSHPAHGTQTQP
                     LEGDPESHARVVGRALLARRARVSS"
     gene            3085..3450
                     /locus_tag="VM692_02530"
     CDS             3085..3450
                     /locus_tag="VM692_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HUQ51068.1"
                     /translation="MTTVTGHTSAIRAGKAIGTDVYDAAGNKIGEVKDIVLDKTSNNI
                     LFAVVSFGGVLGMGEKYHPVPWSELDYDPDQGGYVVSFTADQLKSAPADSIEALTKGD
                     GLAYRDRAYTHYGTRPYWH"
     gene            complement(3490..>5278)
                     /locus_tag="VM692_02535"
     CDS             complement(3490..>5278)
                     /locus_tag="VM692_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ51069.1"
                     /translation="IVLSRAGREPAALASIVSQGNFSVVERPVRASTLVSLIRASLRA
                     RSRQYQVRKHLAQQEHTQRFIREAEQRFRLLIENITDYAIFMVDVEGRVASWNSGAQS
                     LLGYESHEILGQPAAGLFEDEGATLEREMQEARATGRAKSTGWRLRKDGTRLFVEGAL
                     TAVYDDQGELLGYSKLMKDITDRRRIENEREQLLQSERFARSEAERSDRMKDEFLATL
                     GHELRTPLNAILGWSQVLSRANGLTAEAADGLKVIERNARAQAQIIEDLLDMSSIISG
                     KVRLAMQRVDVAAVLDSCVKAVQPAADAKEIRLTVRLESVATQVSGDPNRLQQVFWNL
                     LTNAVKFTPKGGEVTVKVAPSGADLEVTVADNGEGINAAFLPHVFERFRQADATSTRR
                     HGGLGLGLSIVKQLVELHGGSITASSGGPGKGSTFRVVLPPMLNESFFTALVDGRRAA
                     ALATQQGAVVPNIGLGGVKVLVVDDEPDARSLLERLLQDREATVTTAASAKEALEALA
                     RDPPDVLISDIGMPKEDGYALMRRIRKLNGTNGKLPAIALTAYARPEDRDKALEAGYQ
                     RHLAKPVEANTLIAMVAELGKREHGRPPH"
BASE COUNT          840 a         1805 c         1765 g          868 t
ORIGIN      
        1 cgtggagacg gctgccggcg agcgccgccg cgtcgtcgcc ggcgacgtca gcgtgaggag
       61 cggctgatgg cggtgctcgt cgacatcggc aacacgcgca tcaagtgggc attgctcgag
      121 cgcggccggc tcaccggccg cggcagcgcc gtgcaccgcg attcgctcga tgctgcgatc
      181 gccgcgctcg acgcggcgct gccggccaag ccgcagatca tcgccagcaa tgtcgcgggc
      241 gacgtcgccg ccgcgcgtct cgaggccctc gttgcagcgc gaccgggcgc atcgctcgag
      301 ctcgtatcga cgtcggccga gcgcttcggc gtgcgttgcg cttacgcgga tccgagcagg
      361 ctcggcgtcg atcggtgggt cgccgtcctg gcggcgcatc acgcgcagcg agcccgcgcc
      421 gcatgcgtct tgagcgcggg cactgccgtg acgttcgacg ccgtcgacgc cgccggtctg
      481 catctcggcg ggctcatcat ccccggcgca agcctgctcg ccgcggcgct cgaccggcat
      541 acgagcaaca tcgggcgcac cgcgccggca acggccgtcc cagagggcct cgagctgctc
      601 ggccgcaaca ccgataaggc ggtcggtcac ggggcatggc tcgcgatcgc ggcggcgctc
      661 gatcgtgccg tcgcgacgat tgccgacgcg ctccgcacta cgcccgtcgt ctacttgacc
      721 ggcggcgacg cggacgcgct gcgcggctgg ctggccacgc gggtcgagct gcgagcagac
      781 ttggtgctcg aggggctcca gttgtttgca ggcgcacgag cggaggcggg tcgctgatgc
      841 gcaatttctt tctcgcgctc gtgctcgcga acctcggctt cgcggcctgg agcgcgtggt
      901 tcgcggcgcc acagcgctcg ggccggccgg ccgacgacgg gctgccggcg ttgacgctcg
      961 tcagcgaggt gccggccgat ctgcgcccga gcggcgcccc ccttcggggc gtcgtggtcg
     1021 agccggccga cgcgtcggag tcggccaccg cgtccgagtc ggcctccgcc gaacgaccga
     1081 cgaccgccgc ggcaacggac acggcagcgc cctcgagggc tgcgcctggc gcggagccgc
     1141 cgccgagcga gcagccgctc gcgaccgcag cggatcgaag cacaacggcc gcggcacgct
     1201 gcgcgagcgt cgggccgttt cgcgagctgt cgcaagttgc cactgccgcg gccgcgttgc
     1261 gcgccgaggg ctatcaaccg cagcagcgcg tcgccgaagg cgagatctgg atcggctatt
     1321 gggtctacat cggcgggatc gcgaccgagg ccgcagccaa cgagattctc gcgaaagtgc
     1381 gcgacggtgg catcgccgat tcgcaagtca ttccgaacag cgacagcggt aacctcgtgt
     1441 cgctcggcgt gttcagcgag atcagcggcg tgagccgtcg gcgcgagcaa gtgcgcgcgc
     1501 tcggcttcga gccgcaagtc gtcgatcgca cgcgccgcgc gaccgtgtat tggatcgacg
     1561 tctcgctcgg ccccgaccaa acgctcgact acgatgcgct gcagacgccg gggcggatca
     1621 gtcgcctcga gcagcgcgat tgcgagcagt cgcccggctg acgagctgat acgcgcggcc
     1681 aaggtttctt gagcgagacg cttggtgcgc tgcaacgtcg ttgcgttatg gtttcctacc
     1741 actttcggca aaccgctccg aggagcaagc aaaatgagac tggtcaattc gacgacatgg
     1801 acgttggcgc tgtgcctgct cggcagcgcg ctggcctttg acgcgcaggc gcaggacgag
     1861 caggcggcgc gcatgcgtac ggcgctcgcg gcaccggcgc ggccggccga ggacaaagag
     1921 cgcgatgcgg ttcgcaagcc gatcgagacc gtgcagttcc tcggcatcaa gaccggccag
     1981 accgtcatcg acgtgatcgc cgccggtggc tggttcaccg aagtgttgtc ggcggccgtc
     2041 ggcccgagcg gcaaagtgct cgcgcagaac cccgagttct tcgtctcgcg gccgggcttc
     2101 gccgaggccg agaagcagcg caacgaccgg ctcggcaacg tgcagcccgt gcacggcgag
     2161 cttgccgcga acggcgtcgt ggctcaggcg gatgccgcga tcacggcatt gaacatgcac
     2221 gacctctaca acggccagaa cggcgaggcc gcagccgtcg cgttcgcgaa aggcgtctac
     2281 gacgcgctga aacccggcgg cacgttcggc gtcatcgacc acgtcggcat cgcggggcaa
     2341 aacaacgcga gcttccaccg catgcagctc gcgcaggcgc gcgacgtgct gacgaaagcc
     2401 ggcttcagga tcgaagccga gtcgaacatc ttggcgaatc ccgcggacga ccacacgaag
     2461 ggtgtgcgcg acatggcacg gcacacggat cagttcttga tccgtgcccg caagccgtag
     2521 ccgggccgct tcggccgaag agccggcgtg ccggccctct gcgccacgcc ggcgctataa
     2581 tgcccggcga tcggcgcgcc cggccgatcg aaaagccacg tctcgagccg gtgtagcaca
     2641 gcggtagtgc agcggttttg taaaccgaag gtcgggagtt cgatcctctc caccggcacc
     2701 atacctcagt cgccttaggt taagcgcgcc cgaattgcgc gcttcgtcgt gacgatgctc
     2761 acggacttag ttgcatgacg caactatgct gcggccatgt tcacgagcac catcattttg
     2821 agcggcacgg tggtgatcga gggcacgacc tttcgctggt ggttgacgga cggtgtcgct
     2881 cagcagctca cggtcagtca cccggcacat gggacgcaga cgcagccgct cgagggcgac
     2941 ccggagtcgc acgcgcgcgt cgtcggtcgc gcgttgttgg cccgtagagc ccgcgtttcg
     3001 tcgtagcctc cgtcccgaag cggcggtgcc attggcatac tgcgtgcatg ccaatgcgcg
     3061 ttgttttgac aaggaggacg aaaaatgacg accgtaactg gacacaccag cgcaattcgg
     3121 gccggcaagg ccattggcac cgatgtctac gacgccgccg gtaacaagat cggcgaggtc
     3181 aaggacatcg tgctcgacaa gacctccaac aatattttgt ttgccgtcgt gagctttgga
     3241 ggagtgctcg gcatgggcga gaaataccat cccgtgccgt ggagcgagct cgactacgat
     3301 ccggaccaag gcgggtacgt ggtctcgttc accgccgatc aattgaagag cgcgccagcc
     3361 gattcgatcg aagcgctgac gaagggtgat ggactcgcgt atcgcgatcg cgcctacacg
     3421 cactacggca cgcggcccta ttggcactga ccgtgctggc gcgcccgccg cgacgcgggc
     3481 gcgccctcat caatgcggag gcctgccgtg ctcccgctta ccaagctcgg cgaccatggc
     3541 gatcagcgtg ttcgcctcga cgggcttcgc gagatgacgc tgatagcctg cctccagcgc
     3601 tttgtcgcga tcctcgggcc gcgcatacgc cgtcagcgca atcgctggca gcttaccgtt
     3661 cgtgccgttc aacttgcgaa ttcgccgcat cagcgcgtag ccgtcttctt tcggcatgcc
     3721 gatgtcgctg atcagaacgt cgggcgggtc gcgcgcgagc gcttctaacg cctctttggc
     3781 cgatgcggcc gtcgtgacgg tcgcctccct gtcttgcagg aggcgctcga gcagcgagcg
     3841 cgcgtcgggc tcgtcgtcga ccacgagcac cttgacccct ccgagcccga tgtttggcac
     3901 gacagcgccc tgctgcgtcg ccagcgccgc ggcgcgccgg ccatccacga gcgccgtgaa
     3961 aaagctctcg ttcaacatcg gcggcagcac gacgcgaaac gtcgagcctt tgccaggacc
     4021 accgctcgac gcggtgatgc tgccgccgtg cagctccacg agctgcttca cgatcgacag
     4081 gccgaggccg agcccaccgt gacgccgcgt cgaggtggcg tcggcttgcc gaaaacgttc
     4141 gaacacatgc ggcaggaatg cggcattgat gccttcgccg ttgtcggcga cggtcacttc
     4201 aagatccgcg ccggagggcg ccaccttgac ggtcacctcg ccgcccttcg gcgtgaactt
     4261 gacggcattc gtcagcagat tccagaacac ctgctgtagc cgattcggat cgccactgac
     4321 ctgcgttgcg acgctctcga gtctgaccgt taggcgaatc tctttcgcat ccgcggcggg
     4381 ctggaccgcc ttgacgcagg agtcgagcac cgcggccaca tcgacgcgct gcatcgcgag
     4441 gcgcaccttg ccggagatga tgctgctcat gtcgagcagg tcttcgatga tctgcgcttg
     4501 cgcccgggca ttgcgctcga tgaccttcaa gccatcggcc gcctcggccg tgagcccgtt
     4561 cgcgcggctc agcacttgcg accagccgag gatcgcattc agcggcgtcc gcagctcgtg
     4621 tcccagcgtc gccaggaact cgtccttcat gcggtccgag cgctcggctt cgctgcgcgc
     4681 gaaccgctcg gactgcagca gctgctcccg ctcgttctcg atgcgccgcc ggtctgtgat
     4741 gtccttcatg agcttcgaat agccgagcag ctccccctgg tcgtcataca cggcggtcag
     4801 cgcaccctcg acgaacagcc gcgtgccgtc cttgcgcagc cgccaacccg tgctcttcgc
     4861 gcggcccgtg gcgcgcgcct cctgcatctc gcgctcgagc gttgcgccct cgtcctcgaa
     4921 cagccccgcg gccggctgcc cgagaatctc gtgcgattcg taccccaata agctctgtgc
     4981 gccgctgttc cagctcgcga cgcgtccttc gacgtcgacc atgaagatgg cgtagtccgt
     5041 gatgttctcg atcagcaacc ggaagcgctg ctcggcctcg cgaatgaagc gctgcgtatg
     5101 ctcttgttgc gcgaggtgct tgcggacctg gtactggcgg cttcgagcgc gcaggctggc
     5161 gcggatcaag ctgacgagcg tcgaggcgcg gaccgggcgc tcgacgacgc tgaagttgcc
     5221 ttgcgacacg atcgacgcca gcgccgcggg ctcgcgcccg gcgcgcgata aaacgatg
//
LOCUS       DATLEE010000117         2246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6772793, whole genome shotgun sequence.
ACCESSION   DATLEE010000117 DATLEE010000000
VERSION     DATLEE010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6772793"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VM692_02540"
     CDS             complement(<1..819)
                     /locus_tag="VM692_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51070.1"
                     /translation="MTRARKSLLSAAALSAWLACACAPAVAQTNLIGYWNPVFHEDVD
                     ERIPGPSIGDYAGLPITDAARLRAEAWDASLLSMPEHQCKPHPSTYGFRGVGVLRIWE
                     DRDENTQQLVKIHTHIAWQEQHREIWMDGRPHPPEYGAHTWQGFSTGTWEGDVLVVKT
                     THLKAGWMRRNGLPLSDRATMTDRFHRYGDIMTHVSMVEDPVYLTEPLVKTNGFLLQP
                     NGTMTPYPCEPVIEVVREPGFVPHYLPGTNPFLNEFGEHYHLRAAATRGGAATAL"
     gene            complement(839..1414)
                     /locus_tag="VM692_02545"
     CDS             complement(839..1414)
                     /locus_tag="VM692_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUQ51071.1"
                     /translation="MVAEIADVRALVHAPCAEHEARRTQFIAALYQRHRRSLLWYLTR
                     LIPNRADAEEVAQEAFVRLLGATHLETDASRARNYLFATATNLARDNYRRRSARAEGA
                     HVALDDLQLEADEPDPTRSLDAERGCRIVASALRSLQPRPRQAFLMYVQEELTYERIA
                     LKLGVSKKTIERDIALTIALCRSRLARWAEA"
     gene            complement(1545..2108)
                     /locus_tag="VM692_02550"
     CDS             complement(1545..2108)
                     /locus_tag="VM692_02550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HUQ51072.1"
                     /translation="MTAVPSIDPRAEAQRLFRQVMGRFATGVTVVTTRLGDETFGMTA
                     NAFMAGSLEPMLCVVSINHTALMHSRLEQAGHFGVSFLSQEQQHLAAHFGGKRLERLV
                     PDFELRGRTPILERAVAAVTADVVSTAACGDHTLFVGSINSLDFAEASRPLLFYGGRY
                     ARLDSRASLGSTRSVAAVDGAPPPEFW"
BASE COUNT          370 a          726 c          755 g          395 t
ORIGIN      
        1 cagcgccgtc gccgcgcccc cgcgggtcgc cgcggcccgc aggtgataat gctcgccgaa
       61 ctcgttcaag aacggattcg tgccgggcag gtagtgcggc acgaaacccg gctcgcgcac
      121 gacctcgatg accggctcgc acgggtacgg cgtcatcgtg ccgttcggtt gcagcaggaa
      181 gccgttcgtc ttgacgagcg gctcggtcag ataaacggga tcttcgacca tcgacacgtg
      241 cgtcatgatg tcgccgtagc gatggaagcg gtccgtcatc gtcgcgcggt cgctgagcgg
      301 caggccgttg cggcgcatcc agccggcctt caagtgcgtg gtcttgacga cgagcacgtc
      361 gccttcccag gtgccggtcg agaagccttg ccacgtatgc gcgccgtact ccggcggatg
      421 agggcggccg tccatccaaa tctcgcggtg ctgctcttgc cacgcgatgt gcgtgtggat
      481 cttcacgagc tgctgcgtgt tctcgtcgcg gtcctcccag atgcgaagca cgccgacgcc
      541 gcgaaagccg tatgtcgacg gatgcggctt gcactgatgc tcgggcatcg acagcagcga
      601 ggcgtcccac gcttcggcgc gcaggcgcgc ggcgtccgtg atcggcaggc ccgcatagtc
      661 gccgatcgac gggccgggga tacgctcgtc gacgtcctcg tggaacacag ggttccagta
      721 accgatcagg ttcgtctgcg ctacggccgg ggcgcatgcg caggccagcc aggcgctcag
      781 cgcggcggcg gaaagcaaac tctttcgtgc acgagtcatc gcgacaagtc ctcgctgatc
      841 aagcttccgc ccatcgtgcc agcctcgacc ggcataaagc gatggtcaac gcgatgtcgc
      901 gctcgatcgt cttcttgctg acgccgagct tgagcgcgat gcgctcatac gtcagctctt
      961 cttgaacgta catcagaaag gcttgccgcg gccgcggttg cagcgaacgg agcgccgagg
     1021 cgacgatgcg gcagccgcgc tccgcgtcga gcgagcgagt gggatccggc tcgtcggcct
     1081 cgagctgcaa gtcgtcgagc gcgacgtgcg cgccttcagc acgcgccgag cgccggcggt
     1141 agttgtcgcg ggcaagattg gtcgctgtcg caaacagata gttgcgcgcg cggctcgcgt
     1201 cggtctcgag gtgcgtcgcg ccgagcaagc ggacgaacgc ttcttgggct acttcctccg
     1261 cgtcggcgcg gttcggtatc aagcgcgtga ggtaccacaa caggctgcgg cggtggcgct
     1321 gatacagcgc agcgatgaac tgcgttcggc gcgcctcgtg ctccgcgcac ggtgcatgaa
     1381 cgagcgctcg aacgtctgca atttccgcga ccattgttcc ccccggtctc ttcgttgcgc
     1441 gccaccgttc gtgcggtgct cggttgttgt gccatagggc gacgacacgc cactaatgct
     1501 ttcttgatca cgggtatgct gcgaaggcat tgctcgacgc aggctcacca gaactcgggc
     1561 ggcggggcgc cgtcgaccgc cgccacgctg cgcgtacttc caaggctcgc gcgcgagtcg
     1621 aggcgcgcgt agcgcccgcc gtagaaaagc aacggccgcg acgcttccgc gaagtccaag
     1681 ctattgatgc tgccgacgaa taaggtgtgg tcgccgcacg cggccgtgct gacgacgtcg
     1741 gccgtgactg ccgcgacggc tcgctcgagg atgggcgtgc ggccgcgcag ctcgaaatcg
     1801 ggaaccaggc gttccagacg tttacccccg aaatgcgcgg ctaggtgctg ctgctcctgg
     1861 ctcaagaacg acacgccgaa atggccggcc tgttcgagcc gcgagtgcat cagggccgtg
     1921 tgattgatcg agacgacgca gagcatcggc tcgagcgacc ccgccatgaa ggcgttggcc
     1981 gtcatgccga acgtctcgtc gccgaggcgc gtcgtgacca cggttacgcc cgtggcaaag
     2041 cgccccatga cctggcgaaa cagccgctgc gcctcagcgc gtgggtcgat gcttggaact
     2101 gccgtcatgc cgctccctcc cgagcgggaa gcctcgcatg cgcgagcggt cctggctaat
     2161 gccgttttaa tagctgtgat gccggttagg catcaccgga gagtcagttc ggtagagcga
     2221 acacgaacag gttgttgccg aggctc
//
LOCUS       DATLEE010000118         4420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_131202, whole genome shotgun sequence.
ACCESSION   DATLEE010000118 DATLEE010000000
VERSION     DATLEE010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131202"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="VM692_02555"
     CDS             complement(<1..1726)
                     /locus_tag="VM692_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51073.1"
                     /translation="MRARTALFVACGFTTLCCGASAAAQSTQSVTAGELVVEPATLIS
                     LGFEWYVEGDADRDAAVAVAYRRAGGGGEWHDSLPLLRVQNERTFYVETLNYTAPNMF
                     AGSVFELDENTDYEVRFTLTDPDGVGGNAQRVVNVRTRAEPQPFAGGTTYHVYPFGYE
                     GPKQEPAFTGLLAAYYKNSLGGDWSRAAPPRVQPGDTLLVHAGVYQDFDRFSYSHEIR
                     TQFGTCCGTPWDGTFYLTQDGTADRPIAIKAAGDGEVVFDGDGNNVLFNVMGADYTYF
                     EGITFRNTNLAIEAGQKSIAGTVGLTVKHSKFEDVGVVVHSDWSGSKNFYIADNVMNG
                     RNDPTYLIGWFSIPPWNAVPGQLEKQRMRSYYAVSIYGSGHVVAYNKVTNFHDGIDHA
                     TYGMPDGYPNTPRDRMPVSNDFYGNDVSNMHDNCFESDGGMHNMRVFRNRCFNAGVGA
                     MSPQPIFGGPVYFIRNVVYNSVYGPLKIQADPSGILVYQNTYVGEIQQITPASNMHFR
                     NNLIMGTNARPVLFAIDTHTPYSSSDYNGFHVNEGAEYSFQWNSPPRGGPVDFTSPRE
                     VHRYKTLAE"
     gene            1820..2698
                     /locus_tag="VM692_02560"
     CDS             1820..2698
                     /locus_tag="VM692_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HUQ51074.1"
                     /translation="MKLVITGASGQLARGAAERVLKSAAPEDLILATRTPAALTSFAA
                     RGVTVRRADFAEPQSLRTAFSGAERLLLVSATDLDRRTTQHRAALEAAKAAGVRHVVY
                     TSGLKPEPPNPAVVAPSHHATEQALTQSGLAWTILRNSLYADYQLPEAQRAIETGELV
                     HNRGTGLVAYVAREDCAAAAAAVLLQDGHDRAVYEITGHERFAAHELATLYAELGGGL
                     VQARALDDAAFIATLVGNGTGDNHLRYGAELVASFGRSIREGYMDSCTDAVAMLTGRE
                     PLALRKVLAPLSLRTP"
     gene            complement(2727..>4420)
                     /locus_tag="VM692_02565"
     CDS             complement(2727..>4420)
                     /locus_tag="VM692_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51075.1"
                     /translation="RSALQQMPAARIVRALDAGAMLAIAMSMNRDERLAVADYLGTDA
                     PDAAVPASAYCTNRAVKLAPTSRAGWNGWSPTQDNARYQVAERAGLTAAQVPRLELEW
                     AYGFAGDVTAFAAPVVVDGHVFVGSASGLVQALAADSGCVKWTWQADGPVRAAPLVAP
                     RDGGHVLLFGDFTGWFYAVDAATGALVWRTRVEAHDSTRLTGAAAVHDGVVYVPVSSW
                     EESRAGDPAYPCCTFRGSVVALRIGDGSQLWQTYLTDVPRELGKNGTGAPLYGPSGAA
                     VWSAPTIDAARGLLYVTTGDNYTEPVTPTSDAVVALALADGRVAWLKQITAGDAYNGS
                     CNRDHRSNCPYEPGPDSDFGSSAILLPGGARLLAGQKSGIVFALDATKRGEILWQTRV
                     GEGGTNGGVQWGMATDGELVFATVSDVGRTRSAGNPFDTRRYILDPKRGGGLTALRVA
                     DGSRAWHFASPPCRDAAPAGCSPAQPGAVTLIPGVVFATSNDGHVRAHATDSGRVLWD
                     FDTAREFATVNGVPARGGSLDGQGVVVADGLVLVSSGYPRNGGMPGNALLAFRAN"
BASE COUNT          695 a         1491 c         1527 g          707 t
ORIGIN      
        1 actcggcgag cgtcttgtat cgatgcacct cgcgcggcga cgtgaagtcg acggggccgc
       61 cgcgcggcgg cgaattccac tggaacgagt actccgctcc ttcgttgacg tggaagccgt
      121 tgtagtccga cgagctatac ggcgtgtgcg tgtcgatcgc gaacagcacg ggccgcgcgt
      181 tcgtgcccat gatcaggttg ttgcgaaagt gcatgttcga cgccggcgta atctgctgga
      241 tctcgccgac gtacgtattc tggtagacga ggattcccga cggatcggct tgaatcttca
      301 gcgggccgta cacgctgttg tagacgacgt tgcgaatgaa gtagacgggc ccgccgaaga
      361 tcggctgcgg gctcatcgcg ccgacgccgg cgttgaagca gcgattgcgg aacacgcgca
      421 tgttgtgcat gccgccgtcg ctctcgaagc agttgtcgtg catgttcgag acgtcgttgc
      481 cgtagaagtc gttcgacacg ggcatgcggt cgcgcggcgt gttcgggtag ccgtccggca
      541 tgccgtaggt cgcgtggtcg atgccgtcgt ggaagttcgt gaccttgttg tacgcgacga
      601 catggcccga gccgtagatc gacaccgcgt agtacgacct catgcgctgt ttctcgagct
      661 ggcccggcac ggcgttccac ggcgggatgc tgaaccaacc gatcaagtac gtcgggtcgt
      721 tgcggccgtt catgacgttg tcggcgatgt agaagttctt cgagcccgac cagtcgctgt
      781 gcacgaccac gccgacgtcc tcgaacttcg aatgcttgac cgtcaagccg acggtgcccg
      841 cgatgctctt ttgcccggct tcgatggcca aattcgtgtt gcgaaacgtg atgccttcga
      901 aataggtgta atcggcgccc atgacgttga acaggacgtt gttgccgtcg ccgtcgaaca
      961 cgacctcgcc gtcgccggcg gctttgatcg cgattgggcg atcggccgtg ccgtcctgcg
     1021 tcagatagaa cgtgccgtcc cacggcgtgc cgcagcaggt gccgaactgc gtccggatct
     1081 cgtggctgta gctgaagcgg tcaaagtcct ggtagacgcc ggcgtgcacg agcagcgtgt
     1141 cgccgggctg cacgcgcggc ggggccgcgc ggctccagtc gccgccgagc gagttcttgt
     1201 agtacgcggc gagcaagccc gtgaacgccg gctcctgctt cggcccttcg tagccgaacg
     1261 gatagacgtg ataggtcgtg ccgcccgcaa acggctgcgg ctccgcgcgt gtgcgcacgt
     1321 tgacgacgcg ctgcgcgttg ccgccgacgc cgtcagggtc cgtcagcgtg aagcgcactt
     1381 cgtaatcggt gttctcgtcg agctcgaaaa cgctgccggc gaacatgttg ggcgccgtgt
     1441 aattcagcgt ctccacatag aacgtgcgct cgttctgcac gcgcaacagc ggcaggctgt
     1501 cgtgccattc gccgccgcct ccggcgcgac gataggcgac cgcaacggcc gcatcgcggt
     1561 cggcatcgcc ttcgacgtac cactcgaagc cgagcgaaat cagggtcgcc ggctccacga
     1621 cgagctcgcc ggccgtgacg ctctgcgtgg attgcgccgc cgcgctcgcg ccgcagcaga
     1681 gcgtggtgaa tccgcaagct acaaaaagag cagttcgtgc gcgcatgaag cccccaaaag
     1741 attggtcttg atcgccggcg agctgcaaga ataagcctcc gctacggccg cgaccaagcg
     1801 cgcggcaggg gggtgcgcaa tgaagctcgt catcaccggc gcttcggggc aactcgcgcg
     1861 cggagcggcg gagcgcgtgc tcaagagcgc tgcgcccgaa gacctcattc tggcgacgcg
     1921 cacgccggcg gcgctcacgt cgttcgcggc gcgcggcgtt accgtgcgac gcgcggattt
     1981 cgccgagccg cagtcgctgc gcaccgcgtt ctcgggcgcc gagcggttgc tgctcgtcag
     2041 cgcgacggat ctcgaccgcc ggacgaccca gcaccgtgca gcgctcgagg ccgccaaggc
     2101 cgccggcgtt cggcacgtcg tctacacgtc ggggctcaag cccgagccgc cgaaccccgc
     2161 ggtcgtcgca ccgagccacc atgcaacgga gcaggcgctg acgcagagcg ggctcgcgtg
     2221 gacgatcctg cgtaacagcc tctatgccga ctaccaacta ccggaagcac agcgagcgat
     2281 cgaaacgggc gagctcgtcc acaaccgcgg cacgggcctc gtggcctatg tcgcgcgcga
     2341 ggactgcgcc gccgccgcgg ccgctgtcct gttgcaagac gggcacgatc gcgccgtcta
     2401 cgagattacg ggccacgagc gtttcgcagc gcatgagctc gcgacgctct acgcggagct
     2461 cggcggcggc ctcgtgcagg cacgcgcgct cgatgacgcg gccttcatcg cgacgctcgt
     2521 cggcaacggg accggcgaca accatctgcg ttatggcgcg gagctcgtcg cgtcgttcgg
     2581 tcgctcgatc cgcgaaggct acatggacag ctgcaccgat gccgtggcga tgctgacggg
     2641 tcgcgagccg ttagcgttgc gcaaagtgct cgcgccgctg agtctgcgca ctccttagcg
     2701 cggcgcttag cgctgaagca acgccgtcag ttggcgcgaa acgccagcag cgcgttgccc
     2761 ggcatgccgc cgttgcgcgg atagccggag ctcacgagca cgaggccgtc ggccacgacg
     2821 acgccttgac cgtcgagcga gccgccgcgc gccggcacgc cgttgacggt cgcgaattcc
     2881 cgcgcggtgt cgaagtccca gagcacgcgg ccgctgtccg ttgcgtgcgc gcggacgtgg
     2941 ccgtcgttcg acgtcgcgaa cacgacgccg ggtatcagcg tcacggcgcc cggttgcgcg
     3001 gggctacagc ccgcaggtgc ggcgtcgcgg cagggcggcg acgcgaagtg ccacgcgcgg
     3061 ctgccgtcgg cgacgcgcag cgccgtcaag ccgccgccgc gcttcgggtc gagaatgtag
     3121 cggcgagtgt cgaacgggtt gccggcggaa cgcgtgcgtc caacgtcgga gaccgtcgcg
     3181 aacacgagct cgccgtcggt cgccatgccc cattgcacgc cgccgttcgt gccgccttcg
     3241 ccgacgcgcg tctgccaaag gatctcgcct cgtttcgttg cgtcgagcgc gaagacgatg
     3301 ccggactttt gtccggcgag caggcgcgcg ccgccgggca acagaatcgc tgacgagccg
     3361 aagtcggagt ccgggcccgg ctcatacggg cagttcgagc gatgatcgcg attgcacgag
     3421 ccgttgtaag cgtcgccggc cgtgatctgc ttcagccacg cgacacggcc gtccgcgagc
     3481 gcgagcgcga cgacggcgtc gctcgtcggc gtcacgggct cggtgtagtt atcgcccgtc
     3541 gtcacgtaga gcaagccgcg cgccgcgtcg attgtcggcg ccgaccatac ggccgcgcct
     3601 gacggcccgt acaacggcgc gcccgtgccg ttcttgccga gctcgcgcgg cacgtcggtg
     3661 agataggttt gccagagctg cgagccgtcg ccgatgcgca gcgcgacgac gctgccgcgg
     3721 aatgtgcagc atgggtacgc gggatcgccg gcgcgcgatt cctcccacga cgagaccggc
     3781 acgtacacga cgccgtcatg cacggcggcc gcgcccgtga gccgcgtgga gtcgtgcgct
     3841 tcgacgcgcg tgcgccagac gagcgcgccg gtcgcggcgt cgacggcgta gaaccagccc
     3901 gtgaaatcgc cgaacagcaa cacgtgcccg ccgtcgcgcg gcgcgacgag cggcgccgcg
     3961 cgcacggggc cgtccgcttg ccacgtccac ttgacgcagc ccgagtcggc ggccagcgcc
     4021 tgcacgagcc cgctcgcgct gccgacgaac acgtggccgt cgacgacgac gggcgccgcg
     4081 aatgccgtga cgtcaccggc gaaaccgtac gcccactcga gctcgaggcg cggcacctgc
     4141 gcggcagtca atcccgcacg ctcggcgact tgatagcgcg cgttgtcttg tgtcgggctc
     4201 cagccgttcc agccggcgcg cgaggtcggg gcgagcttta cggctcggtt cgtgcaatag
     4261 gcggacgccg gcaccgcggc atcgggtgcg tcggtgccga gataatccgc tacggcgagc
     4321 cgctcgtcgc ggttcatcga catcgcgatc gcgagcatcg cgccggcgtc gagcgcgcgc
     4381 acgatgcgcg ccgcgggcat ctgctgcaaa gccgaccgat
//
LOCUS       DATLEE010000119         2216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8262418, whole genome shotgun sequence.
ACCESSION   DATLEE010000119 DATLEE010000000
VERSION     DATLEE010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8262418"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VM692_02570"
     CDS             <1..472
                     /locus_tag="VM692_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HUQ51076.1"
                     /translation="VTSGTFVPSQSLTFAGIELRIDGAPAAGDSFTVTPAAQRDVFTT
                     LERLATAVEGEAGNAADRAELHSNIGQRLADLDQGIGHIIAARGEIGARVRALDQQED
                     LNADFAVHLKTTLSAVRDLDYAEALSRLSQELFGLDAAQQAFARAQNLSLFKFL"
     gene            644..2179
                     /locus_tag="VM692_02575"
     CDS             644..2179
                     /locus_tag="VM692_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HUQ51077.1"
                     /translation="MAQVINTNVLSLNAQRNLTTSQSSLATALQRLSSGLRINSAKDD
                     AAGLAISDRFTTQIRGLNQAVRNSSDGISLAQTAESALGELTNNLQRIREIAVQAANA
                     SNSASDRAALDQEVQQRLAEVERIATQTAFNGQKVLNGTFGNATFQVGANVGETISVG
                     LATSMRTIDIGKTADYINLTTQYSTALAVGQQGTGVDDTTLAAGEVTIAIGSGQAISV
                     GASVAGSADGQSIESAYAKAAAINAAGIGGLTATADTTLVVAWDDYNDTGYSLSLNGN
                     AIFTNYDGSTTALTGSAAAAAINANASDSGVTASYDSVSGNMTLTAVDGRDIDVSQTI
                     AAGGTANLGLNGTGGGTNNATNAALAFNTAVGATTAVTAKGSIRLVSASTIAIGGSSP
                     AAIGFGVGGSLALGSSALNSASVTTVANANTTIGRVDAALTSVSSLRSTFGAIQNRFE
                     STIASLSAVSENLEASRSRILDADFAAETAALTRAQILQQAGVAILAQANTAPQSVLA
                     LLQ"
BASE COUNT          416 a          725 c          739 g          336 t
ORIGIN      
        1 cgttacgtcg gggacgttcg taccgtcgca gtcgctaacg ttcgccggca tcgagctgcg
       61 catcgacggc gcgccggcgg cgggcgactc gttcaccgtg acaccggccg cgcagcgcga
      121 cgtcttcacg acgctcgaga ggctcgcaac ggccgtcgag ggggaagccg gcaacgcagc
      181 cgaccgcgcg gagctgcaca gcaacatcgg ccagcgactc gcggatctcg atcagggaat
      241 cggccacatc atcgcggcgc gcggcgagat tggcgcgcgc gtgcgcgcgc tcgaccagca
      301 agaagatttg aacgccgact tcgcagtgca cttgaagaca acgctctcgg ccgtgcgcga
      361 tctcgattac gcagaagcgc tgagccggtt gtcgcaggag ctattcgggc tcgacgctgc
      421 gcaacaagcg ttcgcgcgcg cgcagaacct ctccttgttc aaattcctct gattcggcgc
      481 gccggctgcg gtcggcgcgt gacgaaggtc tcaattcctc cctaaaggat cgtttggtcc
      541 gcccgataca ggtgccagac gacagggctt cagccggacg gcagctcggt cgcaagtggc
      601 caatgacggc cgtctggcaa ccaaccttta ggagaggatg acaatggctc aagtaatcaa
      661 caccaacgtg ctgtcgttga atgcgcagcg caatttgacg acttcgcagt cgtcgctggc
      721 gacggcgttg caacgcctgt cgtcgggctt gcgcatcaac agcgcgaagg acgacgccgc
      781 aggtctcgcg atctcggatc gtttcacgac gcaaattcgc ggcctcaacc aggccgtgcg
      841 taactcgagc gacggtatct cgctggcgca gaccgcggag agcgccctcg gcgagctcac
      901 gaacaacctg cagcgcatcc gcgagatcgc ggttcaggcg gccaacgctt cgaacagcgc
      961 ctcagaccgc gcggcgttgg atcaggaagt tcaacagcgt ctcgcggaag tcgagcgcat
     1021 cgcgacgcag accgcgttca acggccagaa ggtgttgaac ggcacgttcg gcaacgcgac
     1081 gttccaagtc ggcgccaacg tcggtgagac gattagcgtc ggtctcgcga cgagcatgcg
     1141 cacgatcgac atcggcaaga cggccgacta catcaacttg acgacgcagt acagcacggc
     1201 gttggcggtc ggccagcaag gcacgggcgt cgacgacacg acgctggctg ccggcgaggt
     1261 cacgatcgcg atcggctccg gccaagcgat cagcgtcggt gcgtcggtcg cgggctccgc
     1321 ggacggccaa tcgatcgaga gcgcgtacgc caaggctgcg gcgatcaacg ccgcgggcat
     1381 cggcggtctt acggctaccg ccgacacgac gttggtcgtc gcgtgggacg actacaacga
     1441 cacgggctac tcgctgtcgc tgaacggcaa tgcgatcttc acgaactatg acggttcgac
     1501 gaccgcactg acgggctccg ccgccgcggc cgcgatcaac gcgaacgcgt cggactcggg
     1561 cgtgaccgcg agctatgaca gcgtctcggg caacatgact ttgaccgcgg tcgacggccg
     1621 cgacatcgac gtgagccaaa cgattgcagc gggcggcacg gccaacctcg gcctgaacgg
     1681 cacgggcggc ggtaccaaca atgcgacgaa cgcggccctc gccttcaaca cggcggtcgg
     1741 cgcgaccacg gccgtgacgg ccaagggttc gattcggttg gtgtcggcgt cgacgatcgc
     1801 gatcggcggc tccagcccgg cggcaatcgg cttcggcgtg ggcggctcgc tcgcgctcgg
     1861 tagctcggcg ctgaactcgg cgtccgtgac gacggttgcc aatgcgaaca cgacgatcgg
     1921 ccgtgtcgac gcggccctga cctcggtcag ctcgctgcgc agcacgttcg gcgcgatcca
     1981 gaaccgcttc gaatcgacga tcgcgagctt gtcggcggtc agcgagaacc tcgaagcgtc
     2041 ccgcagccga attctcgacg cggacttcgc ggccgagacg gcggctctga cgcgcgcgca
     2101 gattctgcag caggccggcg tcgcgattct ggcgcaggcc aacacggcgc cgcaaagcgt
     2161 cctggccctc ttgcagtaaa cgcgtaagtg tcgcgggagg gaacccgcga ggacag
//
LOCUS       DATLEE010000120         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1623088, whole genome shotgun sequence.
ACCESSION   DATLEE010000120 DATLEE010000000
VERSION     DATLEE010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623088"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VM692_02580"
     CDS             <1..519
                     /locus_tag="VM692_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ51078.1"
                     /translation="EAFALIERLLEAGAEPNARTREFPHERRYIVSVVGSVAWVDLTG
                     QTPFLRAAAAGDLRVMRLLLAHGADPNIATTAGTTPLMVAAGVNWAVAETFDEGPAAL
                     LEAVRLAHELGNDVIEYLAANGARLDVRDREGRSPLDWAAGVFLATHPPVRKPETIAL
                     LERLLEAPSTTP"
     gene            533..1555
                     /locus_tag="VM692_02585"
     CDS             533..1555
                     /locus_tag="VM692_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ51079.1"
                     /translation="MSDDNRQPPQGDDPWKSPEGDEPICAERRSLLRAASALAAGAAL
                     FDPAQAQQAGGTPSAAAPLSIPGFAPARIDVGGGVTINAAKGGSGPPLLLLHGAPLTL
                     YTWRDVAPQLAEDFTVVAADLRGYGDSSKPQGAADHSNYSKRAMASDQVAVMQHFGFD
                     RFAVVGQDRGGRVTHRMALDHADKVTKAVLIDIVPTYYLYTHVNIDFVQAYFHWFNYL
                     RAAPAPENELLAAQAGRQVSGAQAEYQRANGTPEGVHGMCEDYRAGASIDLQHDAADL
                     DSKIRCPLHVLWAENGAMDKLYDVLAIWRERARTVTGKGMPGGHNMQEGAPAEVLAEL
                     RTYLGA"
     gene            1580..>2381
                     /locus_tag="VM692_02590"
     CDS             1580..>2381
                     /locus_tag="VM692_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51080.1"
                     /translation="MAYAIAMLGLVARADAATPLEAYGALPTVENIVISPDGTRLAFV
                     RTQGEERFVAVVSLATGETLAGARAGDVKLRSIEWAGDGHVLMVGSVTKDVTWLSGGK
                     TEWSLLSVLDVGANTVTAVPKQGADNPNIISDVPMVREIDGRVVLFVPGAHLRDYLLP
                     ALYRYDVESGAQTLLERGLRSTRQWFVDDRGNIVARIHYDDAVSSWTIEARQSGGQYR
                     VVEQGFSTVYEPRILGYGFDHDSMLVSYPSNDSWKWALVPLRDEEPDLG"
BASE COUNT          407 a          807 c          791 g          376 t
ORIGIN      
        1 gaggcgttcg cgctgatcga gcgcctgctc gaggccggcg ccgaaccgaa cgcacgcacg
       61 cgcgagtttc cgcacgagcg ccgctacatc gtctccgtcg tcggctccgt cgcttgggtc
      121 gatctgaccg gccaaacgcc gttcctgcgt gcggcggcgg ccggcgatct gcgcgtcatg
      181 cgcttgctgc tcgcgcatgg cgcggacccg aacatcgcaa cgaccgccgg cacgacgccg
      241 ctgatggtcg cggccggcgt caactgggcc gtcgcggaga ccttcgacga aggccccgcg
      301 gcgctgctcg aagccgtgcg gctcgcgcac gagctcggca acgacgtcat cgagtattta
      361 gctgcgaacg gcgcccgact cgacgttcgc gaccgcgagg gccgctcgcc gctcgattgg
      421 gcggccggag tatttctcgc gacgcatccg cccgtccgca aacccgagac gatcgccctg
      481 ctcgaacgcc tgctcgaggc accatcgacc actccttaat tgaggttctg acatgagcga
      541 cgacaaccga cagcccccgc agggggacga cccttggaag tccccggagg gggacgaacc
      601 gatctgcgcg gagcgccgca gcttgctgcg cgccgcgtcc gcgctcgcgg ccggcgcggc
      661 gctcttcgat ccggcgcagg cgcaacaagc gggcggcacg ccgtcggcgg ccgcgccgct
      721 gtcgattccg ggcttcgcgc cggcgcgcat cgacgtcggc ggcggcgtga cgatcaacgc
      781 cgccaaaggc ggtagcgggc cgccgttact gctgctgcac ggcgcgccgc tgacgttgta
      841 cacgtggcgc gacgtcgcgc cgcagctcgc cgaggacttc accgtcgtcg cggccgatct
      901 gcgcggctac ggcgacagca gcaagccgca aggcgctgcg gaccattcga actactcgaa
      961 gcgcgcgatg gcttcggacc aggtcgccgt catgcagcac ttcggcttcg accgcttcgc
     1021 cgtcgtcggt caggaccgcg gcggccgcgt tacgcatcgc atggcgctcg atcacgccga
     1081 caaggtcacg aaggccgtgc tgatcgacat cgtgccgacg tattacctct acacgcacgt
     1141 caacatcgac ttcgtgcagg cgtactttca ctggttcaac tatctgcgcg cagcgcccgc
     1201 tcccgagaac gagctgctcg ccgcgcaagc gggccggcaa gtctcgggcg cgcaggcaga
     1261 atatcagcgc gcaaacggca cgcccgaggg cgtgcatggc atgtgcgagg actaccgcgc
     1321 cggcgcatcg atcgacttgc agcatgacgc ggccgatctc gactcgaaaa ttcgttgccc
     1381 gttgcacgtg ctgtgggccg agaacggcgc catggacaag ctctacgacg tgctcgcgat
     1441 ctggcgcgag cgcgcgcgca ccgtgacggg caaaggcatg cccggcggcc acaacatgca
     1501 agagggcgcg cccgcggagg tcctcgccga gctgcgaacc tacctgggcg cctagcgaag
     1561 tctcgcaatg agtcgcctgg tggcatatgc aatcgccatg ctggggctcg tcgctcgagc
     1621 ggacgcagcc acgccgctcg aagcgtatgg cgcgctcccg accgtcgaaa acatcgtgat
     1681 ctcaccggac ggcacgcgcc tcgccttcgt acgcacgcaa ggcgaagagc gcttcgttgc
     1741 agtcgtgtct ctcgcgaccg gcgagacgct tgcaggggcg cgcgccggtg atgtgaagct
     1801 gcggagcatc gaatgggcgg gagacgggca cgtcctcatg gtcggctcgg tcacgaagga
     1861 cgtgacttgg ctctccggcg ggaaaaccga gtggtcgttg ctcagcgtgc tcgacgtagg
     1921 cgcaaatacc gtaacggcgg tcccaaaaca gggcgcagac aatccgaaca tcatcagcga
     1981 cgtgccgatg gtgcgcgaga tcgacggacg cgtggtcctg ttcgttccgg gcgcccatct
     2041 cagggactac ttgttaccgg ccttgtatcg ctatgacgtc gaaagcggcg cgcagacgct
     2101 gctcgagcgt ggcctgcgat cgacgaggca atggttcgtc gacgatcgag gcaacatcgt
     2161 ggcccgcatc cactacgacg acgcggtcag ctcttggacc atcgaagcgc ggcagtctgg
     2221 cggccagtat cgtgtcgtcg aacaaggctt ctctacggtc tacgagccgc gcatcttggg
     2281 ttacggcttc gaccacgatt ccatgctcgt gagctatccg agcaacgact cctggaaatg
     2341 ggcactggtg ccgttgcgcg acgaggaacc cgacctagga t
//
LOCUS       DATLEE010000121         2759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3048957, whole genome shotgun sequence.
ACCESSION   DATLEE010000121 DATLEE010000000
VERSION     DATLEE010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3048957"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VM692_02595"
     CDS             <1..284
                     /locus_tag="VM692_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HUQ51081.1"
                     /translation="YRAPNLARALRWLKEAGVWVVGADAAAPRSLYETKLAPPVAMVL
                     GGEGRGVRRLTRDLCDELVYIPMSGAVESLNVSVATGIVLFELKRQVPR"
     gene            405..854
                     /gene="rpsF"
                     /locus_tag="VM692_02600"
     CDS             405..854
                     /gene="rpsF"
                     /locus_tag="VM692_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HUQ51082.1"
                     /translation="MRHYEVVFLVHPDQSEQVPAMMERYRNMIEGASGKIHRQEDWGR
                     RQLAHSIAKVHKAHYLLLNIECDKPTLDELVGAFRFSDAVLRYLVISRDEAVTEPSFM
                     AKAREEEEEREAQSATRSAPRERPARPPQDFNSPEALPDEPAEVERE"
     gene            879..1103
                     /gene="rpsR"
                     /locus_tag="VM692_02605"
     CDS             879..1103
                     /gene="rpsR"
                     /locus_tag="VM692_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020584246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HUQ51083.1"
                     /translation="MARYFRRKKFCRFTAEGVKEIDYKDLATLKAYVTETGKIVPSRI
                     TGTSAPYQRQLATAIKRARYLALLPYTDQH"
     gene            1154..1615
                     /gene="rplI"
                     /locus_tag="VM692_02610"
     CDS             1154..1615
                     /gene="rplI"
                     /locus_tag="VM692_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019640985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HUQ51084.1"
                     /translation="MEIILLQKVDNVGGIGDLVRVKSGYARNYLIPQGKATLATPENK
                     AKFESRRAELEAKAAAELAAAQARAKKLEGLTVKIEMQAGTEGKLFGSVGTVDIGEEI
                     AKRGIEVERSEIRLPDGPLRVVGEHRVDLHLHADVNVGITVVIEAVEAANA"
     gene            1652..>2759
                     /gene="dnaB"
                     /locus_tag="VM692_02615"
     CDS             1652..>2759
                     /gene="dnaB"
                     /locus_tag="VM692_02615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HUQ51085.1"
                     /translation="MAEAPADLARRAAERLRLPPHSVEAEQSLLGGLMLDQRAWDQIA
                     DVVAADDLYRADHRLIFTAVAALVERNQPPDAVTVSEHLQRLGQLEAAGGLPYLARLV
                     EDTPSAANIRAYGRIVRDHAMLRKLIEIGGDIAASAHATEGLTAAEIVDRAEARVFEI
                     AEHGHGRGSGFVSLKNILPKTIDRLDFLSHSTSEITGVSTGFAEMDKWTAGLQRGDLV
                     IIAGRPSMGKTSLAINIAENAALGHKTPVAIFSMEMSAEQLSFRMLSSIGHIGQQRLR
                     TGRLLEEDWPRVDSAVSMMSEAPIFIDDSGGLSPTEVRARARRLKREHGLGLIVVDYL
                     QLMQVTGTVENRATELSEISRSLKALAKELEVPVI"
BASE COUNT          541 a          851 c          908 g          459 t
ORIGIN      
        1 tctatcgcgc gccgaacctc gcgcgcgctc tgcgctggct caaggaggcg ggtgtctggg
       61 tcgtcggcgc cgacgccgcg gcgccgcggt cgctctacga aacgaagctc gcgccgcccg
      121 tcgccatggt gctgggcggg gaagggcgag gtgtgcgccg gctgacgcgg gatctttgcg
      181 atgagctcgt gtacatcccg atgtccggcg ctgtcgagag cctcaatgtc tcggttgcca
      241 cgggaatcgt gctgttcgag ctgaaacggc aggtcccaag ataaggcggc tggttgagcg
      301 gcctccgccg accgggtagt atggcggccc cccgaaccga acaatcgggg aactccttgc
      361 ctcaccgcat gacgggaggc tgttcaaccc atagggagat ctcattgaga cactacgaag
      421 tcgtgtttct ggtccatcct gaccagagcg agcaagtgcc cgcgatgatg gagcgttatc
      481 gcaacatgat cgaaggcgcg agcggcaaga tccatcggca agaagactgg ggccggcggc
      541 aattggcgca ttcgatcgcc aaggtgcaca aggcgcacta cttgctgctg aacatcgagt
      601 gcgacaagcc gacgctcgac gagctcgtcg gtgcgttccg cttcagcgat gccgtgttgc
      661 gttatctcgt catcagccgc gacgaggccg ttaccgagcc ttcgttcatg gctaaggcgc
      721 gtgaagagga agaggagcgc gaggcgcaat cggcgacgcg ttcggcgccg cgcgagcgtc
      781 cggcgcgacc gccgcaggac ttcaatagcc cggaagcgct ccccgacgag cctgccgagg
      841 tcgagcgcga atagcgctat agaacaggag acacgaacat ggcccgttat tttcgacgca
      901 agaagttctg ccgtttcaca gccgaaggcg tgaaggagat cgactacaag gatctcgcaa
      961 cgctgaaggc gtacgtcacc gagaccggca agatcgtgcc gagccgcatc acgggcacga
     1021 gcgcgccgta tcagcggcag ttggcgacgg cgatcaaacg cgcgcgttac ctcgcgttgc
     1081 tgccttacac ggaccaacat tagctacagt tgagttccgg gcgctgcgac gcgcccggga
     1141 cgagagttga gacatggaaa tcatattgct gcagaaagtc gacaacgtcg gtggcatcgg
     1201 cgacctcgtt cgcgtgaagt cgggctacgc gcggaattat ctgatcccgc agggcaaggc
     1261 cacgcttgcg acgcccgaaa acaaggcgaa gttcgagtct cgtcgtgcgg agctcgaagc
     1321 gaaagccgcg gccgagctgg cggccgcgca agcgcgggcc aagaagctcg agggcctcac
     1381 ggtcaagatc gagatgcagg ccggcaccga aggcaagctg ttcggctcgg tcggcaccgt
     1441 cgacatcggc gaggagatcg cgaagcgcgg catcgaggtc gagcgcagcg agattcgctt
     1501 gccggatggc ccgttgcgcg tcgtcggcga gcatcgcgtc gacctgcacc tccacgccga
     1561 cgtcaacgtg gggatcacag tcgtgatcga ggccgtcgaa gcggccaacg cctaaaacta
     1621 accaggagcc tcggcggctc cgaagataag gatggccgaa gcacccgccg atctagcccg
     1681 tcgggccgcc gagcgcctgc ggctcccgcc gcactcggtc gaagccgagc agtcgttgct
     1741 cggcggcctc atgttggacc aacgcgcctg ggaccagatc gcggacgtcg tcgccgccga
     1801 cgatctctac cgcgccgacc atcggctgat cttcacggcc gtcgccgcgc tcgtcgagcg
     1861 caatcagccg cccgacgccg tcaccgtcag cgagcacctg cagcgcctcg ggcaactcga
     1921 ggcggctggt ggcttgccgt atctcgcgcg cctcgtcgag gacactccga gcgccgccaa
     1981 catccgcgct tacggccgca tcgttcgcga ccacgcgatg ctgcgcaagc tcatcgagat
     2041 cggcggcgac atcgccgcga gcgctcatgc gaccgaaggg ctgacggccg cggagatcgt
     2101 cgatcgcgcc gaagcgcgag tgttcgagat cgccgagcat gggcacggac gcggctcggg
     2161 tttcgtctcg ttgaaaaaca tactgccgaa gacaatcgac cgcctcgact tcttgagtca
     2221 ttcgacgagc gagataaccg gcgtctcgac gggtttcgcc gagatggaca aatggacggc
     2281 aggcttgcag cgtggtgacc tcgtcatcat tgccgggagg ccatccatgg gcaaaacctc
     2341 cctggctatc aacattgccg agaatgcggc gcttggtcac aagactccgg ttgcaatctt
     2401 cagcatggaa atgtctgcgg agcagctaag ctttcgcatg ctgagctcga tcggtcacat
     2461 tggccagcaa cggttgcgga ccggcaggct acttgaagaa gactggccgc gcgtcgactc
     2521 cgcggtatcg atgatgtcgg aggcgccgat tttcatcgac gacagcggcg ggttgtcacc
     2581 gaccgaagtc cgcgcgcgcg caaggcggct gaagcgcgag catggactcg gcttgattgt
     2641 cgtcgactat ctgcagctga tgcaggtgac cggtacggtc gaaaatcgtg caacggagct
     2701 ctcggagatc tctcgatctt tgaaggcctt ggccaaagag ctcgaagtgc cggtcatcg
//
LOCUS       DATLEE010000122         3674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8264767, whole genome shotgun sequence.
ACCESSION   DATLEE010000122 DATLEE010000000
VERSION     DATLEE010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8264767"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VM692_02620"
     CDS             complement(<1..471)
                     /locus_tag="VM692_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HUQ51086.1"
                     /translation="MSDATPLTDLIRDAGSGDEAAMRRLFDVTYAELRQLARLRLKGG
                     GRGTLLDTTALVHESYLRFAKLEQLGVEDRRKFFAYASKVMRSVIVDFVRERVAVRRG
                     GGAEHVTLDTAIGDGAHADGGAEEILRVHEALSSLAQYGERLVRVVEMRYFAGMT"
     gene            complement(655..1296)
                     /locus_tag="VM692_02625"
     CDS             complement(655..1296)
                     /locus_tag="VM692_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51087.1"
                     /translation="MIPDTNHELKLYQSGDLFSIKVPGRGELMNSRVHGSEKALADLA
                     CARLGGNPKPRLLIGGLGMGFTLAAALKALGPEAEVVVAELVPEVVTWNRTLIGAPAG
                     HPLADPRSTVYVGDVADVILRQPKSFDAILMDVDNGPEAMVRRENDWLYGPSGLRATR
                     AALRPGGVLAVWSASADPAFAKCLQRAGFDVREHTVRPHRAGKGSRHVIWIAA"
     gene            complement(1323..2084)
                     /locus_tag="VM692_02630"
     CDS             complement(1323..2084)
                     /locus_tag="VM692_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51088.1"
                     /translation="MSKSTALLTIAFGGLVSASLQTIEAQQTAARPDFTGVYYPFNPD
                     AAPAASRGAGTPPRPVAVIDGRAGRPATAPKLAPEYLAKWEQMRQSRMAGSSDSDPNA
                     NCLSAGMPGMMSMGYGMELQQTADKITMYGELNDVYRRIYLDGRTPSQRTLDDPTFGG
                     YSVGHWEGDTLVVNTVAIREDTLLDLFSPHSDQLSVQERIRFTAPGILEDRLTSTDPK
                     ALLEPFTQVRTYKKAAPPNDELREFSCAEGLLSVK"
     gene            complement(2108..2476)
                     /locus_tag="VM692_02635"
     CDS             complement(2108..2476)
                     /locus_tag="VM692_02635"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51089.1"
                     /translation="MKALAVVTVVLAATPALAHHSNSAFDPDKVVVLKGTVEDWRWTN
                     PHVWIVLSVDDGKGAQTVWEIEGRPPGILARNGWSKNTFKKGDVITVDFSPARDGSHT
                     GLSTRVTLADGTVLTQATPQ"
     gene            complement(2617..2940)
                     /locus_tag="VM692_02640"
     CDS             complement(2617..2940)
                     /locus_tag="VM692_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HUQ51090.1"
                     /translation="MLVTFHTKAYGDITMFGDVAKQLLGLMGHSGTIPSAIKAEDVPD
                     ALARLESALAQRQAADAAEARKDVDNRDAPEAPRKVTLSQRAVPLRDLLRAAAAKKTD
                     VMWDS"
     gene            complement(2993..>3674)
                     /locus_tag="VM692_02645"
     CDS             complement(2993..>3674)
                     /locus_tag="VM692_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HUQ51091.1"
                     /translation="DKPEPLYPSLLLGAIDLTPYEVTELYNTLANGGFHAPLRAVRAI
                     IDGRGNELERHSLVLEQVADPVAVYAVNQGLVQVMQRGTGVPARRLLAPGFRAAGKTG
                     TSDGSRDSWFAGFGNDHLVVAWVGNDANDDVRLTGGTGAAQIWGRVMRSVDAAPYDPP
                     APEGAENLWIDYETGLATDRECPNAVYLAVSERAVPPKAVECGSTRTRVGARIREWFK
                     NRLRDGVF"
BASE COUNT          603 a         1236 c         1184 g          651 t
ORIGIN      
        1 cgtcatgccc gcgaaatagc gcatctcgac gacgcgtacc agccgctcgc cgtactgcgc
       61 gaggctactc agcgcttcgt ggacgcgcaa tatctcttct gcgccgccgt ccgcgtgcgc
      121 gccgtcgccg atcgccgtgt cgagcgtcac gtgctcggcg ccgccgccgc ggcggactgc
      181 tacgcgctca cgcacgaaat cgacgatgac cgagcgcatg accttggagg cgtaggcgaa
      241 gaacttgcgc cgatcttcga cgccgagctg ctcgagcttt gcgaatcgca gataggattc
      301 gtggacgagg gccgtcgtgt cgagcaacgt gccgcgcccg ccgcctttga gccgcaagcg
      361 cgcgagctga cgcagctcgg cgtacgtgac gtcgaacagt cgtcgcatcg cggcttcatc
      421 gccgctgccg gcgtcgcgaa tcaagtcggt cagcggagtt gcgtcgctca agggccaata
      481 cgagccacga ctgcacagag tgcatcgagc ttgctcgaac tcatgggctc ggtcctccgt
      541 ctgcgttgta gttgttctcg gagcttccga tcggaaaaag ctacccggcg agtctagtca
      601 atggcgcgtc cagcgcatat accggtatcg cgccgagcga ccaaaaggcc ccgtttacgc
      661 ggcgatccag atgacgtgac gcgatccctt gcccgcccgg tgcggacgca cggtgtgctc
      721 gcgcacgtcg aagccggcgc gctgcaagca ctttgcgaat gccggatccg cgctcgccga
      781 ccagacggcc aacacgccac cgggccgaag cgcggcccgt gtcgctcgca atccgctcgg
      841 gccgtagagc cagtcgtttt cgcggcggac catcgcttcg gggccgttgt ccacgtccat
      901 caggatggcg tcgaagctct taggctgccg caggatcacg tctgctacgt cgcccacgta
      961 gacggtgctg cgcgggtccg cgagcggatg gcccgccggc gcgccgatca atgtgcggtt
     1021 ccaagtcacg acttcgggca cgagctccgc cacgacgacc tcggcctcgg ggccgagcgc
     1081 tttgagcgcc gccgccaacg tgaatcccat cccgaggccg ccgatcagca ggcgcggctt
     1141 cgggttgcca ccgagccgcg cacacgccag atccgccaga gctttctcgg agccgtgcac
     1201 gcgactgttc atcagctcgc cgcgaccggg aaccttgatc gaaaatagat cgccggactg
     1261 gtatagcttc agctcgtgat tcgtgtcggg aatcacagcg gtaccgagga gaacgcgcgg
     1321 aatcacttga cggacaacag cccctcggca caggagaact cgcgcagctc gtcgttcggc
     1381 ggcgctgcct ttttgtaagt tcgaacttgt gtgaacggct cgagcaatgc cttcgggtcc
     1441 gtcgacgtga gccgatcttc gagaatgccg ggagccgtga accgaatccg ctcctgcacg
     1501 ctgagctgat cgctgtgcgg cgagaacagg tcgagcaacg tgtcctcgcg gatcgcaaca
     1561 gtgttgacga cgagcgtgtc gccttcccaa tggccgaccg aatacccgcc gaaggtgggg
     1621 tcgtcgagcg ttcgctgcga cggcgtgcgg ccatcgagat agatgcgccg atacacgtcg
     1681 ttcagttcgc cgtacatcgt gatcttgtct gccgtctgct gcagctccat gccgtagccc
     1741 atcgacatca ttcccggcat gccggccgac aggcaattcg cgttcggatc ggagtcggat
     1801 gagcccgcca tccgcgattg ccgcatctgc tcccacttcg cgaggtactc gggcgcgagc
     1861 ttcggcgccg tcgcggggcg ccccgcacgc ccatcgatga cggcgacggg ccgcggcggc
     1921 gtcccagcgc cgcgcgaggc cgccggcgca gcgtccgggt tgaacggata gtagacaccc
     1981 gtgaagtcgg ggcgcgcagc cgtctgctgc gcctcgattg tttgcagcga tgcactgact
     2041 aagccgccga atgcgatcgt caacaaagcc gtgctcttcg acatgcgcct ctccgatgaa
     2101 ccgcgactta ctgcggcgtc gcctgcgtca gcacggtgcc gtcggccaac gtgacgcgcg
     2161 tgctgagacc cgtgtgactc ccgtcgcgcg ccgggctgaa atcgacggtg atgacatcgc
     2221 ccttcttgaa cgtgttcttc gaccaaccgt tacgagccaa aatgccgggc ggacggcctt
     2281 cgatctccca cactgtctgc gcgcctttgc cgtcgtcgac cgagagaacg atccagacgt
     2341 gcgggttcgt ccaccgccag tcctcgaccg tgcccttgag cacgacgacc ttgtcggggt
     2401 cgaacgccga attgctgtgg tgagcgagcg cgggcgtcgc cgcgagaacg acggtgacga
     2461 cggccaaggc tttcatagcg accctcccgg tggcgcctta tccgttccgc cggactttcg
     2521 gccgaggggc gcgcccagcg gaacgccggg atagtaccgc taccgcggcg cgaaactcca
     2581 taatccgaat atgcgcgcga tgatcgccgt gtgacctcag ctatcccaca ttacgtcggt
     2641 cttctttgcc gcggcggccc gaagcaggtc gcgcaacggc acggcgcgct gcgatagcgt
     2701 taccttgcgc ggcgcttcgg gggcatctcg gttgtcgacg tctttgcgcg cttcggcggc
     2761 atcggccgct tggcgctgcg cgagggcgct ttcgagccgc gcaagcgcat cgggcacatc
     2821 ctcggctttg atcgcgctcg ggatcgtgcc gctgtggccc atcaaaccca acagttgctt
     2881 ggcgacgtcg ccaaacatcg tgatgtcgcc gtacgccttc gtgtggaatg tcacgagcat
     2941 gaagcctcct tgtcgttgtg gggcggcgag ataagttcga tttcgtggcg cactagaaca
     3001 ctccgtcgcg caaccggttc ttgaaccatt cgcggatacg cgcgccgacg cgcgtgcgcg
     3061 tgctgccgca ctccacggct ttcggcggca cggcgcgttc cgacacggcg aggtagacgg
     3121 cgttcggaca ctcgcggtcc gtcgcaagcc cagtctcgta gtcgatccag aggttctcgg
     3181 caccctcggg ggccggtgga tcgtacggcg cggcgtcgac gctgcgcatg acccgccccc
     3241 agatctgcgc cgctcccgtt ccgcccgtca gccggacgtc gtcgttggcg tcattgccga
     3301 cccaggccac cacgaggtgg tcgttgccga agccggcaaa ccagctatcg cgcgagccat
     3361 ccgaggtacc ggtcttgcct gcggcacgaa agcccggggc cagcagccgt cgagccggca
     3421 cgcccgtgcc tcgctgcatg acctgcacca atccctggtt caccgcgtag acggcgacgg
     3481 ggtccgcgac ttgctcgagg acgagcgagt ggcgctcgag ctcgttgccc cgcccgtcga
     3541 tgatggcgcg cacggcgcgc aacggcgcgt ggaagccgcc gttcgcaagc gtgttgtaga
     3601 gctccgtgac ttcgtatggc gtgagatcga tagctccgag cagcagcgaa ggatagagag
     3661 gctccggttt atcg
//
LOCUS       DATLEE010000123         6807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4604207, whole genome shotgun sequence.
ACCESSION   DATLEE010000123 DATLEE010000000
VERSION     DATLEE010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4604207"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VM692_02650"
     CDS             <1..640
                     /locus_tag="VM692_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ51092.1"
                     /translation="RELLHFHGRLLGLAARGLGTRVEGLLLRVGLEDAANRPLRTYSK
                     GMTQRAGLAAALLGSPELVFLDEPTSGLDPLGRLLVRDVIDELRDGGATVFLNSHLLG
                     EVEATCDRVAFVTNGRVVHELTLASAQGTLDVELRLGGADDAVLQGLTAFGSGVKRDD
                     VVVRMHATAETMLPDLARWLVARGVAIYEMRCRRKTLEEWFVEVMGDDQKPG"
     gene            646..1497
                     /locus_tag="VM692_02655"
     CDS             646..1497
                     /locus_tag="VM692_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ51093.1"
                     /translation="MQGTLLIARLTWLETRRRRIALAALICGLLFLAVFGTAMYFGFR
                     LARAGGPTGVDPVNAVEMRVAATILTVAGLYVVNFLVTAVAILLPIDSLSGEISSGVI
                     QTLASKPVDRAAIVVGKWLAYLVMTAAYLALMGGGVVLIMRLFADYVAPAVLAPLALM
                     LLGAAVMLSITIAGGVRLSTITNGIVAFGFYGMAFIAGWMEQILSMFGNTAARHVGTV
                     VSLLSPADSMWRRAAFEMQPLATVALQNGPFGVVSVPSAAMIGWAVLFCVVVVGFAAW
                     QFRRRPL"
     gene            complement(1510..1845)
                     /locus_tag="VM692_02660"
     CDS             complement(1510..1845)
                     /locus_tag="VM692_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51094.1"
                     /translation="MRTTIAAVSIGLLVSSTASTQESLSYAAISQRHAVVRMTEAELA
                     KMRAEGECLAGIKALNALPMDQFQPNIEWLNIRTQAMLALSKPCEVLVMLEAAHDALA
                     TSNAPGLKN"
     gene            complement(1871..2428)
                     /locus_tag="VM692_02665"
     CDS             complement(1871..2428)
                     /locus_tag="VM692_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ51095.1"
                     /translation="MSIRIAAAALCLVASAGLQAQAPTAAPRELTALDYIEIQQLVAR
                     YARAIDTCSNNGYDYADLFTADGAFIPSFEGKQLAPIQGRDRLAAVSGGGSNGCKDVP
                     WIEQGVRHIYTNHVIVPTPDGASATVDMLMIGLGGDPNKIEYDGHYEDTYVKTPQGWR
                     FKQRVHHALLREGQRVTPAPAAQKN"
     gene            2599..3597
                     /locus_tag="VM692_02670"
     CDS             2599..3597
                     /locus_tag="VM692_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HUQ51096.1"
                     /translation="MQAVIQSGYGPIERVLSVGRVPKPVPNDDEVLVRVRAASMHPDV
                     WHVIVGFPFALRFMGNGVLTPRPIPGTDIAGVVEAVGKGVTRFKPGDEVFGECAKHGW
                     INGGAYAEYVAVPQDYLALKPSNVSFEQAAAVPTAGTIALNNLGGVARPKRGSVLING
                     AGGCMGTIALQIAKADGAYVTAVDCAAKLPMLRALGADRVIDYEKEDYRGNGERYDFI
                     LDVVQFRSPDEYRPSLTPQGEYIPIGHAHYDDSANRLLGDLPKFLGLVFKAMRDPEKR
                     KTFKFPTKPEALEVFRSLLAAGKLTPVIGKTFGLSGAVEAMKCMQQGELGRVIITP"
     gene            complement(3608..4807)
                     /locus_tag="VM692_02675"
     CDS             complement(3608..4807)
                     /locus_tag="VM692_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51097.1"
                     /translation="MSGKRRTAIARAAAMSLTATLLLAAAASAQVTDELRAKIAAQKP
                     PAGVEPLPIDLFTTKDFYLDSEHWADPRYTRCNTPWRIDEMWVDNFVGEWGDCNDGLT
                     AEQLKSPHPYKTAEEHYEALLAQAKANGGPTIYDRQHPPPDWDGYYNSRTLQQQQWSF
                     GNNVQAASLMQVLTPEYQRRFTQALYHVGVSNSAQWVSSTCYPEGLIRWWAQGIRNIQ
                     VTVTPHQVQLLSGTALNFLRQILIGQKHVSLISQWYGETVGFWDDETLVAWTDHVQGW
                     TMSHALPEFSSELEAIEVFTRDAQRNIHLEITIYDPVAFVAPLKLEAIYSYVAGPESD
                     NRYQWKECQQALWNVNGRLQPVAPGTVIEYRIPDWYDRPWARSWEQHFEEGMQRPEPG
                     EEIFKFE"
     gene            complement(4804..5199)
                     /locus_tag="VM692_02680"
     CDS             complement(4804..5199)
                     /locus_tag="VM692_02680"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HUQ51098.1"
                     /translation="MEQRKINPWKWQDRYGFSQGIEVTNASRVLYCAGQASVDDQGNP
                     VHAGDMRKQIEQAFLNLEAVLKAAGYSLANVVRLNYYTTDVDAFLGAADVFGSRLAAA
                     ASQPPGTLLGVTKLAFPGLMVEIEATAVK"
     gene            complement(5210..5770)
                     /locus_tag="VM692_02685"
     CDS             complement(5210..5770)
                     /locus_tag="VM692_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ51099.1"
                     /translation="MSRHIVRALLAGAAVLQVSLLAPAQAQVTMETLLDRIQIEDLLV
                     KYYYDLAMSRAHEMSEYFTEDAMLDVDGTIANGRAEIEKLYGGGARARDPNAAPRPHN
                     GMLLTNPVIEVKGNRAQAHVIWTGVTNKGVGQPPNLYEQGREDTELVKVNGKWLISKR
                     YISSDSGTPDRFDQTFKQRDNPLPPL"
     gene            complement(5817..6215)
                     /locus_tag="VM692_02690"
     CDS             complement(5817..6215)
                     /locus_tag="VM692_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51100.1"
                     /translation="MAAKTRTRRRPLSLADVRELALSLPEVEETTAYGMPAFKAGKTR
                     FAGQPIERADVEPNSLGVRVSFEERERRLTARPDLYYLTEHFASYPAVLARLTNMRRE
                     ELAELLGTAWRYAMEAQPPPKKKRKRRAAR"
     gene            complement(6263..6700)
                     /locus_tag="VM692_02695"
     CDS             complement(6263..6700)
                     /locus_tag="VM692_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HUQ51101.1"
                     /translation="MAKYSGGCEHVHTTADKDPIDNHTCHCSVCKRVTGQAATHVTFF
                     NHGDLKVDNAAGLKRQPFNDQNPKGPLELCTCAECGKPIMLDDKQKRIRVIVPNLMGH
                     TKIADTTYHAFFDAKAGNKKPSDGKPVYEGLRPDFVWPKGVPA"
BASE COUNT         1037 a         2152 c         2284 g         1334 t
ORIGIN      
        1 gcgcgagctc ttgcactttc acgggcgctt gctcggcctc gctgcgcgcg gcctcggcac
       61 gcgcgtcgag gggctgctct tgcgcgtcgg gctcgaggac gcggcgaacc ggccgctgcg
      121 cacctacagc aagggcatga cgcagcgcgc cggtctggcg gcggcgttgc tcggttcgcc
      181 ggagctggtc tttctcgacg agccgacgtc gggcctcgat ccgctcgggc gcttgctcgt
      241 gcgcgacgtc atcgacgagc tgcgcgacgg cggtgccacc gtgttcctga actcgcacct
      301 gctcggcgag gtcgaggcga cctgcgatcg cgtggcattc gtgacgaatg ggcgcgtcgt
      361 gcacgagctt acgctcgcga gcgcgcaagg cacgctcgat gtcgagctgc ggttgggggg
      421 agccgacgac gcggtgctcc aggggctcac ggcgttcggt agcggcgtga aacgcgacga
      481 cgtggtcgtt cgcatgcatg ccaccgccga aacgatgctg ccggatctcg cccgctggct
      541 cgtcgcgcgc ggcgtcgcga tctacgagat gcgttgtcgc aggaaaaccc tcgaggagtg
      601 gttcgtcgag gtcatgggcg acgatcagaa acccggttga cgaacatgca aggcacgttg
      661 ctgatcgccc gcctgacctg gctcgagacg cgccgccgcc gcatcgcgct tgcggcgctg
      721 atttgcgggc tgctgtttct cgcggtcttt gggacggcga tgtacttcgg tttccgcttg
      781 gccagagccg gtgggccaac gggcgtcgat ccagtgaacg cggtcgagat gcgagtcgcg
      841 gcaacgatct tgaccgtggc cggtctctac gtcgtgaatt tcctcgtcac ggccgtcgcg
      901 atcctactgc cgatcgattc gctgtccggc gagatcagct ccggcgtcat tcaaacgctc
      961 gcgtcgaagc ccgtcgatcg ggccgcaatc gtagtgggta agtggctcgc ctacctcgtc
     1021 atgacggcgg cgtatctcgc gctgatgggc gggggcgtcg tgctgatcat gcggctgttc
     1081 gcggactacg tcgcgcccgc cgtgctcgcg ccgttggctt tgatgctgct cggcgccgcg
     1141 gtcatgctgt cgatcacgat cgcgggtggc gtgcggctct cgacgatcac gaacggcatc
     1201 gtggcgttcg gcttctacgg catggcgttc atcgccggct ggatggagca gatcctgagc
     1261 atgttcggca acacggccgc gcgccacgtc ggcacggttg tgagcttgct gagccccgcg
     1321 gactccatgt ggcggcgcgc cgctttcgag atgcagccgc tcgcgaccgt cgcgctgcag
     1381 aacgggccgt tcggcgtcgt ctccgtcccg agcgccgcga tgatcggctg ggccgtgctg
     1441 ttctgtgtcg tggtcgtcgg cttcgccgcc tggcagttcc gccgcaggcc gctgtgaccg
     1501 ccggcgccgt cagttcttga ggcccggcgc gttcgacgtg gcgagcgcgt cgtgcgccgc
     1561 ctcgagcatc acgagcacct cgcacggctt cgagagcgcg agcatcgctt gcgtgcggat
     1621 gttcaaccac tcgatgttcg gctgaaactg atccatcggc agcgcgttca gcgccttgat
     1681 gccggcgaga cactcgccct cggcgcgcat cttcgcgagc tcggcctcgg tcattcgcac
     1741 gacggcgtgc cgttgactga tggcggcgta gctcaagctc tcctgcgtgc ttgccgtcga
     1801 agagacgagc aagcctatcg agaccgcggc gattgtcgtt cgcatggatt ggctccggct
     1861 tagttggtag ctagttcttt tgcgccgccg gggcgggcgt tacgcgttgc ccctcgcgaa
     1921 gcagggcgtg gtgtacgcgt tgtttgaagc gccacccctg cggcgtcttc acgtaggtat
     1981 cctcgtaatg gccgtcgtac tcgattttgt tggggtcgcc gccgaggccg atcatcaaca
     2041 tgtcgacggt cgccgacgcg ccgtccggcg tcggaacgat gacgtggttc gtatagatgt
     2101 ggcgcacgcc ctgctcgatc cacgggacgt ccttgcagcc gttcgagccg ccgccggata
     2161 cggccgcgag ccggtcgcgg ccctggatcg gagctagctg cttgccttcg aacgagggaa
     2221 tgaaggcgcc gtcggccgtg aacaaatcgg cgtagtcgta gccgttgttc gagcacgtgt
     2281 cgatcgcgcg cgcgtaacgc gcgacgagct gctgaatctc gatgtagtcg agcgccgtaa
     2341 gctcgcgtgg cgccgcggtc ggggcctgtg cctgtaagcc ggccgaggcc acgagacaga
     2401 gcgcggcagc tgcaatccga atcgacatag ctctctcctt ggggctcgat gccgctgaga
     2461 ttagcaccgg cgaagcgccc gatacttgac ttgatggcgt actaacactc ttgtacgagc
     2521 gcgagctcga caaagcgcgt gctcgcggta caagagactc gtgaagcagg ggttcaactt
     2581 cgagttatga gcgagctgat gcaagcagtg atccaatcgg gctacggccc aatcgagcgc
     2641 gtgctttcgg tcggacgggt tcctaagccg gtgccgaacg acgacgaggt gctcgtgcgc
     2701 gtgcgcgcgg cctccatgca tcccgacgtc tggcacgtga tcgtcggctt tccgttcgcg
     2761 ctgcggttca tgggcaacgg cgtgctgacg ccgaggccta tcccgggcac cgacattgcc
     2821 ggcgtcgtcg aggctgtcgg caaaggcgtc actcgcttca agccgggcga cgaggtgttc
     2881 ggcgagtgcg cgaagcatgg ctggatcaac ggcggcgcct acgccgagta cgtcgcggtg
     2941 ccgcaagact acctggcact gaagccgagc aacgtgtcgt tcgagcaggc cgccgccgtg
     3001 ccgacggccg gcacgatcgc gttgaacaac ctcggcggag tcgcgcggcc gaagcgtggc
     3061 tcggtgctga tcaacggcgc cggcggctgc atgggaacga tcgcgctgca gatcgcgaag
     3121 gccgacggtg cgtacgtgac ggccgtcgat tgcgccgcga agctgccgat gctgcgcgcg
     3181 ctcggcgccg atcgcgtcat cgactacgag aaggaagact atcgaggcaa cggcgagcgg
     3241 tacgacttca ttctcgacgt cgtgcaattc cgctcgcccg acgaataccg gccgtcgctg
     3301 actccgcaag gcgagtacat cccgatcggc cacgcgcact acgacgacag tgcgaaccgg
     3361 ctcctgggcg acctaccgaa gttcctcggc ctcgtgttca aggccatgcg cgatcccgag
     3421 aagcgcaaga ctttcaagtt tccgacaaag ccggaagcgc tcgaggtgtt tcgcagtcta
     3481 ttggcggcgg gcaagctgac gccggtcatc ggcaagacat tcgggttgag cggagcggtc
     3541 gaagccatga agtgcatgca gcaaggagag ctcggccgag tcatcatcac gccgtgaggg
     3601 cgcgtgctca ctcgaacttg aagatctcct cgccgggctc cggccgctgc atgccttcct
     3661 cgaagtgctg ttcccaactg cgcgcccacg gccggtcgta ccagtcgggg atgcggtatt
     3721 cgatgacggt gcccggcgcg acgggttgca gccggccgtt cacgttccag agcgcctgct
     3781 gacactcttt ccactgatag cggttgtcgg actcggggcc cgcgacgtag gagtagatcg
     3841 cctcgagctt cagcggcgca acgaacgcta cggggtcgta gatcgtgatc tcgaggtgga
     3901 tgttgcgctg cgcgtcgcgc gtgaacacct cgatcgcctc gagctcgctg ctgaactcgg
     3961 gcagcgcgtg cgacatcgtc caaccctgca cgtggtccgt ccaggctacg agcgtctcat
     4021 cgtcccagaa gccgaccgtc tcgccgtacc actgcgagat caggctcacg tgcttctggc
     4081 caatcaagat ttggcgaagg aagttcagcg cagtcccgga cagcagctgc acttgatgcg
     4141 gcgtcaccgt gacctgaatg ttgcggatgc cttgcgccca ccagcggatc aggccttcgg
     4201 ggtagcacgt cgacgacacc cactgcgccg aattgctgac gccgacgtga tagagcgcct
     4261 gcgtgaagcg ccgctgatac tccggcgtca acacttgcat cagcgacgcg gcttggacgt
     4321 tgttgccgaa gctccattgc tgctgctgca gcgtgcgcga gttgtaatag ccgtcccagt
     4381 ccggcggcgg atgctggcgg tcgtagatcg tcggcccgcc gttcgctttc gcttgcgcga
     4441 gcagcgcctc gtagtgctcc tcggccgtct tgtaaggatg cgggctcttc aactgctcgg
     4501 ccgtgaggcc gtcgttacag tcgccccact cgccgacgaa attgtcgacc cacatttcgt
     4561 cgatgcgcca cggtgtattg cagcgcgtgt agcgcggatc ggcccagtgc tccgagtcga
     4621 ggtagaagtc cttcgtcgtg aagaggtcga tcggtaacgg ctcgacgccg gccggcggct
     4681 tctgcgccgc gatcttcgcg cgcagctcgt ccgtgacctg cgccgacgcc gccgctgcga
     4741 gcagcagcgt cgccgtcagc gacatggccg ccgcgcgcgc gattgctgtg cgtcgcttgc
     4801 cgctcacttg acggccgtcg cctcgatttc gaccatgagg cctggaaacg cgagcttcgt
     4861 gacgccgagc agcgtgccgg gcggttgcga cgccgcggcc gccagtcggc tgccgaacac
     4921 gtcggcggcg ccgaggaagg catcgacgtc ggtcgtgtag tagttgagcc gcacgacgtt
     4981 cgcgagactg tagcccgcgg ccttcaacac ggcctcgagg ttcaggaacg cctgctcgat
     5041 ttgcttacgc atgtcgccgg catgcacggg gttgccctgg tcgtcgacgg acgcctgccc
     5101 ggcgcagtac agcacgcggc tcgcgttcgt tacttcgatc ccttgcgaga agccgtatcg
     5161 gtcctgccac ttccaagggt tgatctttcg ttgttccacg cgttggtcct caaagcggcg
     5221 gcaacggatt gtcgcgctgc ttgaacgtct ggtcgaagcg atccggcgtg ccgctgtcgg
     5281 agctgatgta gcgcttgctg atgagccact tgccgttgac cttgacgagc tcggtatctt
     5341 cgcggccctg ctcgtagagg ttcggcggtt ggccgacgcc tttgttcgtc actccggtcc
     5401 agatcacgtg tgcctgggcg cgattgccct tcacctcgat gacggggttc gtcagcagca
     5461 tgccgttgtg cggccgtggc gcggcgttcg gatcgcgcgc tcgtgcaccg ccgccgtaga
     5521 gcttttcgat ctcggcgcgg ccgttcgcga tcgtgccgtc gacgtcgagc atcgcgtcct
     5581 cggtgaagta ctccgacatc tcgtgcgcgc ggctcatcgc aaggtcgtag tagtacttga
     5641 cgagcaaatc ttcgatctgg atgcggtcga gcagcgtttc catggtgact tgcgcttgcg
     5701 ccggcgcgag cagcgatact tgcagcaccg cggcgccggc gagcaaggca cgaacaatgt
     5761 gacgcgacat gcggctctcc tcagtgtggt gctcgaatag tacttccaag cgcgggtcag
     5821 cgtgcggcgc ggcgcttcct cttcttcttt ggcggcggct gagcctccat tgcgtagcgc
     5881 cacgccgtgc cgagcagctc ggcgagctct tcacgccgca tgttcgtcag ccgcgccagc
     5941 acggcgggat agctcgcgaa gtgctcggtc aggtagtaga ggtcggggcg cgctgtcagc
     6001 cgtcgctcgc gctcctcgaa gctgacgcgg acaccgagcg agttcggctc gacgtcggca
     6061 cgctcgatcg gctgacccgc aaagcgcgtc ttaccggcct tgaatgccgg catgccgtag
     6121 gccgtggtct cttcgacctc cggcagcgac aacgcaagct cgcggacgtc cgcaaggctc
     6181 agcggtcgac gccgcgttct agttttggcg gccacgctga cgcgccccac gcccatgggg
     6241 ggcgtgggga gttgtcagag aatcaggctg ggacgccctt cggccaaacg aagtcggggc
     6301 gaaggccttc gtagacgggc ttgccgtccg acggcttctt gttgccggct ttcgcgtcga
     6361 agaacgcgtg ataggtcgta tccgcaatct tcgtgtgacc catcaagttc gggacgatga
     6421 cgcgaatacg cttctgcttg tcgtcgagca tgatcggctt gccgcactcc gcgcacgtgc
     6481 aaagctcgag cggtcctttg ggattttgat cgttgaacgg ttgacgcttg agccccgccg
     6541 cgttgtcgac cttgaggtcg ccgtgattga agaacgtaac gtgtgtcgcc gcttgacccg
     6601 tcacgcgctt gcacacggag cagtggcaag tgtggttgtc gatcggatct ttatccgctg
     6661 tcgtgtggac gtgttcgcag cccccggaat acttcgccat gattcgtcac cttcatgttg
     6721 ttgatgtcgg atggcgtcga ccgcacggtc ggcgacctgg ccccgcgatt atatagaagg
     6781 ggcgaagcgt tgcgtcgatt tcgcgcc
//
LOCUS       DATLEE010000124         2884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_883001, whole genome shotgun sequence.
ACCESSION   DATLEE010000124 DATLEE010000000
VERSION     DATLEE010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883001"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VM692_02700"
     CDS             complement(<1..1008)
                     /locus_tag="VM692_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51102.1"
                     /translation="MAFKKATTGTRVALVTGLAAVLSLVAYAQQGQAPQPPPYTPAEG
                     ARDLKSVLFNWPWHMGMLRGIEEHELAVTLEYRGTGTIQVNGQPCKLDPYEEAREGEL
                     GKSGYRISNAYQFAGSRTQINCTLPNGRKYSNIEAVSGKYAWDEDRPGAGLVPGEGTV
                     KAMPNQVAERLIRLWASPQGAPKAALAGAGIDPLKVFRNPGGLLQEGTAKIGDTSVVW
                     EGNTPIVTFPIPGVSGAVATAELDERFMAKTVTVKHGRDTYEFTYSKYDDYNNPLNRI
                     ETYMAGKMTERKNGQVMRDVDTVVMEAGSVYVVVPVPPSIAPTQPLPVFGSDQQAPKL
                     TS"
     gene            1281..2360
                     /locus_tag="VM692_02705"
     CDS             1281..2360
                     /locus_tag="VM692_02705"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HUQ51103.1"
                     /translation="MEAVRPPIEPMLAKLAAELPPPGGVLYEPKWDGFRALVFKDARS
                     LYIQSRDLRPLDRYFPDLHDALIERVPTSCVLDGEIVIATKHGLDFDELQLRLHPAAS
                     RVAKLAAARPASFVAFDLLAVGRKNLMACTQVERRAQLEELLAEAEPPLYLTPLTRDR
                     DVALDWLERFEGAGLDGVIAKPADASYQPGKRAMLKVKHVRTADCVVAGFRHHKSGND
                     AVGSLLLGLYDDAGVLQHVGVTSSFTMARRRALLKELAPLRRDAMRDHPWRDWAGAAA
                     ESRRMPGAQSRWSAGKNLSWEALRPERVCEVKYDHLQGDRFRHAATFLRWRPDKPPEE
                     CRYDQLETTAPYELADVFSSGSSAS"
     gene            complement(2315..>2884)
                     /locus_tag="VM692_02710"
     CDS             complement(2315..>2884)
                     /locus_tag="VM692_02710"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HUQ51104.1"
                     /translation="VRPTERATVSAPLAWSEIDTSEPGDFTLLSMPGRFAKLGDLHSG
                     MDRSAGSLDALLALYESQGLGDAPWPPHYRKQRGEPPRVQPSKAKRESAAPKRRGAAA
                     PKRGPRRPLIEVGRAKREQDALAGLERWKKRHREAVAYLEEADVLVDAMRGRYSTWTR
                     VRLNLEHVPPQLRPRQEALDPDENTSASS"
BASE COUNT          434 a          957 c          964 g          529 t
ORIGIN      
        1 ggacgtcagc ttcggcgctt gctgatcgct gccgaagacc ggcagaggct gcgtcggcgc
       61 aatgctcggc ggcaccggca cgaccacgta cacgctgccg gcttccatga cgacggtgtc
      121 gacgtcgcgc atgacctggc cgttcttgcg ctccgtcatc ttgccggcca tgtaggtttc
      181 gatccggttc aacggattgt tgtagtcgtc gtacttgctg tacgtgaact cgtaggtatc
      241 gcgcccgtgc ttgacggtga cggtcttcgc catgaagcgc tcgtcgagct cagccgtggc
      301 gacggcgccg gaaacgcccg gaatcgggaa cgtcacgatc ggcgtgttgc cctcccagac
      361 gacggatgtg tcgccgatct tcgcggtgcc ttcctggagc agtccgcccg gattacggaa
      421 caccttcagc gggtcgatgc cggcgcccgc caaagcggct ttcggtgcgc cttgcgggct
      481 ggcccataga cggatcaaac gctcggcgac ctgatttggc atcgccttga ccgtgccttc
      541 gccgggcacg aggcctgcac ccggtcggtc ttcgtcccaa gcgtacttgc cgctgacggc
      601 ttcgatgttc gagtacttgc gaccgttagg cagcgtgcag ttgatctgcg tgcggctgcc
      661 tgcgaattga tacgcgttgc tgatgcggta acccgacttg ccgagctcgc cctcgcgcgc
      721 ttcctcgtac gggtcgagct tgcagggctg gccgttcact tggatcgtgc cggtcccgcg
      781 atactcgagc gtaacggcga gctcatgctc ctcgatgccg cgcagcatgc ccatgtgcca
      841 gggccagttg aataacacgg acttcaagtc gcgcgcgcct tccgcgggcg tgtagggcgg
      901 cggctgcgga gcttgccctt gctgcgcgta ggccaccaat gaaaggacgg ccgcgaggcc
      961 ggtaacgagc gcgacgcgcg ttccggtcgt ggcttttttg aaggccatgg taattccccc
     1021 tataccaagg atgctgcgtt tattcgggat tgatgccgca gcaacgtcac acgagactac
     1081 caccgccggt ccattcgtcc cacttgtccg cgggcggtct acgcgtcggc tacaggaaac
     1141 ttgacaagcg gcccgcccaa gcaaggcggg caaagacgtg ctgtacgcgc ggttagcgtc
     1201 gggaaccgat cgcgtggggt ggtgcagtgg ccgcattcaa cgcacgactt cgctggcatg
     1261 gtcgttgctc gcaacttcgt atggaagccg tgcgcccgcc gatcgaaccg atgctcgcca
     1321 agctcgccgc cgagctgccg cccccgggcg gcgtcctcta cgagcccaag tgggacggct
     1381 ttcgcgcgct cgtgttcaag gacgcgcggt cgttgtacat ccaaagccgc gacttgcggc
     1441 cgctcgaccg gtattttccg gatctacacg acgcgctgat cgagcgcgtg ccgacgagct
     1501 gcgtgctcga cggcgagatc gtcatcgcga cgaagcatgg cctcgatttc gacgagctgc
     1561 agttgcggct gcatccggcc gcatcgcgtg ttgcaaagct ggcggccgcg cggccggcat
     1621 cgttcgtggc cttcgacctc cttgccgtcg gccgcaagaa tttgatggcg tgcacgcagg
     1681 tcgagcggcg tgcgcagctc gaggaactgc tagccgaagc cgagccgccg ctctatctca
     1741 cgccgctgac gcgcgatcgc gacgtggcgc tcgattggct cgagcgcttc gagggtgcgg
     1801 gactcgacgg cgtcatcgcc aagcccgcgg acgcgagtta ccagcccggg aagcgcgcga
     1861 tgctcaaggt caagcacgtt cggacggcgg actgcgtcgt tgccggtttt cggcatcaca
     1921 agtcgggcaa tgacgccgtc ggctcgctgc tgctcggcct gtacgacgac gccggcgtgc
     1981 tgcagcacgt cggcgtcacg tcatcgttca cgatggctcg gcgtcgtgcg ctgctgaagg
     2041 agctggcgcc ccttcggcgc gacgcaatgc gcgatcatcc gtggcgcgac tgggccggtg
     2101 ccgccgcgga gtcgcgtcgc atgcccggcg cgcagagccg ctggagcgcg ggcaagaatc
     2161 tttcctggga ggcgctgcga cccgagcgcg tttgcgaggt gaaatacgac cacttgcagg
     2221 gcgaccgttt tcgccatgcc gcgacgttct tgcgctggcg gcccgacaag ccgcccgagg
     2281 agtgccgtta cgatcagctc gagacgacgg cgccctacga gctcgcggac gtgttctcgt
     2341 ccggatcgag cgcctcctga cgcggccgca gctgcggcgg cacatgctcc aagttcaggc
     2401 gcacgcgcgt ccacgtcgag taacgcccac gcatcgcgtc gacgaggacg tcggcctcct
     2461 cgagataggc gacggcttcg cgatgccgct tcttccagcg ctcgagcccc gcgagcgcgt
     2521 cttgctcacg cttcgcccga ccgacctcga tcagaggacg gcgtggcccg cgtttgggtg
     2581 cggcagcgcc gcggcgcttc ggcgcagccg actcgcgctt tgccttcgac ggctgcacgc
     2641 gaggcggctc gcctcgttgt ttgcggtaat gcggcggcca cggtgcgtcg cccaagcctt
     2701 gcgactcgta gagcgcgagc agcgcgtcga gcgaaccggc ggaccggtcc attcccgagt
     2761 gcagatcccc tagcttcgcg aatcggcccg gcatgcttag gagcgtgaag tcgcccggct
     2821 ccgacgtgtc gatctcgctc caagcgagcg gcgcagacac cgtcgcgcgc tcggtggggc
     2881 gcac
//
LOCUS       DATLEE010000125         3963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_139473, whole genome shotgun sequence.
ACCESSION   DATLEE010000125 DATLEE010000000
VERSION     DATLEE010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139473"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1408)
                     /locus_tag="VM692_02715"
     CDS             complement(62..1408)
                     /locus_tag="VM692_02715"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ51105.1"
                     /translation="MFITKKHLSRRTLLKGGSAAIGLPLLSAMVPAATALAQTAAAPK
                     PRMGFFYLPHGAIMANTRFGAEMNRWTPDKVGRDFDLKPILEPFKPLQKYMTVVSGLG
                     NKPGESSAVHAIVPATWLSATHPRQSHAPFAGVTIDQVAARHIGQDTPLPSLEVATEE
                     EGGGAACDGTYGCSYGRTISFRTPTTPMPMEYEPRKAFEKIFGRGKSDAERRAVSGDY
                     KSLLDMALGEANALKRTLGGEDRALVDDYLDSVREIERRLELLGQRDLTKIDLPDVPV
                     ARPSFDDLLRLQFDLIVAAFQANMTRITSFMMAAEVSNQSYAHVGVPDEFHPVSPHAD
                     NKASMEKLVLIQRYHTQVFNEFMTKLSQIPDGDAGSILDNSIFLYGSNMSNSNQHNQF
                     PLPTLVFGRGAGTIKGNQHLVYPDRTPLANLLFTLLVRAGVPVDKVGDSTAELAEV"
     gene            complement(1410..3887)
                     /locus_tag="VM692_02720"
     CDS             complement(1410..3887)
                     /locus_tag="VM692_02720"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ51106.1"
                     /translation="MTRSDIRLTRRLGPVAMLAGMLSLAGCSEPPPVEAVAQHVETFG
                     RFCTDCHNDAERAGEFSLEHVTAADVAARPELFEKVIRKLRGGLMPPPGEPRPDAEER
                     HELVVALERYLDETAAARGPEPGRVALHRMNRTEYATAVEQLLGVKIDARSMLPADMA
                     SEGFDNVAEVLRVTPTHIEQYVAAARDISIMAVGEKAPAPTRADYRSERGLRTAHVDG
                     LPLGTRDGLVVEHNFPADGTYEINLNISSIPGSELRGYPYGWLEFEHTVAVTIDGAKV
                     FAGNIGGDVDSKALDQGQIKEVEAIKNRFRGIKVDVKAGRRRVGAAFVARSFAEGDYL
                     LQSLVPGEGVPDVPRLYGMEIIGPYEPTGIAEPTESRARIFSCYPQDASEEQTCATQI
                     LTNLSRAAFRRPVTSDDVAPALEFFAQGRAEGDFETGIQKGLMSILASTSFLYRAEPG
                     APPADLPPGSDAGRDARVRATQGAVAAYAITDLELAWRLSFFLWSQGPDETLLGLAAD
                     GTLHEPAVLERQVRRMLSDERSRSLVTNFAFQWLGVRRLETIDPDPRLYPNFDEDLRR
                     AFVTEMELYLDSILRSDASVVDLLTAKHTFVNERLARHYGLPNVRGDQFRRIELDDPH
                     RFGLFGKGSVLMVTSYPDRTSPVLRGAWIMEQILAAPPNPPPPGVETNLAPLETPKSV
                     RERLALHRTQSSCNQCHGVIDPLGQALENFNVIGEWRTHERDTGVKVDSTGITATGQP
                     ANSPEELRAALASDPTLFVHALTEKLLTFALGRGVEYYDMPVVRRIVADAEAQGYSFE
                     AIVLGIAQSAPFRMRSVADDAEGSAGD"
BASE COUNT          620 a         1301 c         1346 g          696 t
ORIGIN      
        1 ggacgtactt gcggcgtccc cggagaaccc tctgcaacga cgtctcgggc gagtgacaag
       61 ctcaaacctc ggcaagctca gccgtactgt cgccaacctt gtcgacaggc acgcccgcgc
      121 gcacgagcaa cgtgaacagc aagttcgcca gcggcgtgcg atccgggtac acgagatgct
      181 gattgccttt gatcgtcccc gcgccacgcc caaacaccag cgtcggcaac ggaaactgat
      241 tgtgctggtt gctgttgctc atgttgctgc cgtagagaaa gatcgagttg tcgaggatcg
      301 acccggcgtc gccgtcgggg atctgcgaga gcttcgtcat gaactcgttg aacacctgcg
      361 tgtgataacg ctggatcaac acgagcttct ccatcgacgc tttgttgtcg gcgtgaggcg
      421 agacgggatg gaactcgtcg ggcacgccga cgtgcgcgta cgactgattg ctgacctcgg
      481 ccgccatcat gaagctcgtg atgcgcgtca tgttggcttg gaacgccgcg acgatgagat
      541 cgaattgcag ccgcagcaag tcgtcgaagc tcggccgcgc gacgggcacg tcgggcaagt
      601 cgatcttcgt cagatcgcgt tggccgagca gctcgagccg ccgctcgatc tcgcggacgc
      661 tgtcgaggta gtcgtcgacg agcgcgcggt cttcgccgcc cagcgtgcgc ttcagcgcgt
      721 tcgcttcgcc gagcgccatg tcgagtaggc tcttgtagtc gcccgatacg gcgcgccgct
      781 cggcgtcgct cttaccgcgc ccgaatatct tttcgaacgc cttgcgcggt tcgtactcca
      841 tcggcatcgg cgtcgtcggc gtgcggaacg agatcgtgcg gccgtagctg cagccgtacg
      901 tgccgtcgca cgcggcgccc ccgccttcct cttccgtcgc gacctcgagc gaaggcagcg
      961 gcgtgtcctg gccgatgtgg cgggcagcga cttggtcgat cgtgacgccg gcgaacggcg
     1021 catggctctg ccggggatgg gtggccgaca gccacgtcgc cggcacgatc gcgtgcacgg
     1081 ccgagctttc gccgggcttg ttgccgagcc cgctgacgac ggtcatgtac ttctgcagcg
     1141 gcttgaacgg ctcgaggatc ggcttcaagt cgaagtcgcg gccgacctta tccggcgtcc
     1201 agcgattcat ctcggcgccg aagcgtgtgt tcgccatgat tgcgccgtgc ggcagataga
     1261 agaagcccat gcgcggcttg ggcgccgcgg cggtctgtgc gagcgccgtc gcggcgggca
     1321 ccatcgccga caacagcggc aagccgatcg cggcgctgcc gcccttcagc agcgtgcgtc
     1381 gcgacaaatg cttcttggtg atgaacatgt cagtctcctg cgctgccttc ggcgtcgtcc
     1441 gcgacgctgc gcatgcggaa cggggcgctc tgcgcaatac cgaggacgat cgcctcaaaa
     1501 ctatagccct gggcctcggc gtccgccacg atgcgacgca cgacgggcat gtcgtagtac
     1561 tcgacgccgc ggccgagcgc gaacgtcagc agcttctccg tcagcgcgtg cacgaacagt
     1621 gtcgggtcgc tcgcgagcgc cgcccgcagc tcctcggggc tattggccgg ctgaccggtt
     1681 gccgtgatgc ccgtcgagtc gacttttacg cctgtgtcgc gctcgtgcgt gcgccactcg
     1741 ccgatgacgt tgaagttctc gagcgcttgg ccgagcggat cgatgacgcc gtgacactga
     1801 ttgcacgacg actgcgtgcg atggagcgcg agccgctcgc gcaccgactt cggcgtctcg
     1861 aggggcgcga ggttcgtctc gacaccgggc ggcggcggat tcggcggcgc cgcgaggatc
     1921 tgctccatga tccacgcacc gcgcagcacg ggcgacgtgc gatccggata cgacgtcacc
     1981 atcagcacgc tgcctttgcc gaataggccg aagcggtgcg gatcgtcgag ctcgatacgg
     2041 cggaattgat cgccgcgcac attcggcaag ccgtagtgac gcgcgagccg ctcgttgacg
     2101 aacgtgtgct tcgccgtcag caaatcgacg acgctcgcgt cgctgcgcaa tatgctgtcg
     2161 agatagagct ccatctccgt gacgaacgcg cggcgcagat cctcgtcgaa gttcggatag
     2221 agccgcggat cgggatcgat cgtttcgagc cggcgcacgc cgagccactg gaacgcgaag
     2281 ttcgtcacga gcgagcggga gcgttcgtcg ctcaacatgc ggcgcacttg ccgctcgagc
     2341 accgcgggct cgtgcagcgt gccgtcggcc gcgaggccga gcagcgtctc gtcgggccct
     2401 tggctccaga ggaagaacga caaccgccac gcgagctcga gatccgtgat cgcgtacgcg
     2461 gcaacggctc cctgcgttgc tctaacgcgg gcatccctgc ccgcgtccga tcccgggggc
     2521 agatcggccg gcggcgcgcc cggctcggcg cggtacaaga agctcgtgct cgcgaggatc
     2581 gacatcaagc ctttctggat acccgtctcg aagtcgcctt cggcgcgccc ttgcgcgaag
     2641 aactcgagcg ccggtgcgac gtcgtcgctc gtgacggggc ggcggaaggc ggcgcgcgag
     2701 aggttcgtga gaatctgcgt cgcgcaggtt tgttcttcgc tggcgtcttg cggatagcag
     2761 ctgaagatgc gcgcgcgact ctcggtcggc tccgcgatgc ccgtcggctc gtacgggccg
     2821 atgatctcca tgccgtagag ccgcggcacg tcgggcacgc cttcgccggg aacgagcgac
     2881 tgcagaaggt aatcgccttc ggcgaagctg cgcgccacga acgccgcgcc gacgcgacgc
     2941 cggccggctt tgacgtcgac cttgatgccg cgaaagcggt tcttgatcgc ttcgacctct
     3001 ttgatctggc cctggtcgag cgccttcgag tcgacgtcgc cgccgatgtt gccggcgaac
     3061 accttggcgc cgtcgatcgt gacggcgacc gtgtgctcga actcgagcca gccgtacgga
     3121 tagccgcgca gctccgagcc cggaatcgac gagatgttga ggttgatctc gtaggtgccg
     3181 tcggccggga agttgtgctc gacgaccaag ccgtcgcgcg tgccgagcgg cagcccgtcg
     3241 acgtgcgccg tgcgcaagcc gcgctcggag cggtagtcgg cgcgcgtcgg ggcgggcgct
     3301 ttctcgccga cggccatgat gctgatgtcg cgcgcggcgg cgacgtactg ctcgatgtgc
     3361 gtcggcgtga cgcgcaatac ctcggcgacg ttgtcgaaac cttcgctggc catgtcggcc
     3421 ggcagcatcg agcgcgcgtc gatcttcacg ccgagcagtt gttcaacagc ggtcgcatac
     3481 tcggtgcggt tcatgcggtg caacgcgacg cggccgggtt ccggcccgcg ggccgcagcc
     3541 gtctcgtcga gatagcgctc gagcgccacg acgagctcgt ggcgctcctc ggcgtcgggc
     3601 cgcggttcgc cgggcggtgg catcaagccg ccgcgcaact tgcgaatgac tttttcgaac
     3661 agctcaggtc tagcggcgac gtcggccgcc gtcacgtgct ccagcgaaaa ctcgccggcg
     3721 cgctccgcgt cattgtggca gtccgtgcag aagcggccga acgtctcgac gtgctgcgcg
     3781 acggcttcga cgggtggcgg ctcgctgcag cccgcgagcg acagcatgcc cgccagcatg
     3841 gcgacggggc cgagcctgcg cgtgagacga atgtcagagc gagtcactaa gcctccctgc
     3901 ccaccaaagc ccgttgtaga tgttcgcgac gaagcgtgcg cggttgttgc cccacttggc
     3961 gtc
//
LOCUS       DATLEE010000126         2714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10503373, whole genome shotgun sequence.
ACCESSION   DATLEE010000126 DATLEE010000000
VERSION     DATLEE010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10503373"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1924)
                     /gene="malQ"
                     /locus_tag="VM692_02725"
     CDS             complement(<1..1924)
                     /gene="malQ"
                     /locus_tag="VM692_02725"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HUQ51107.1"
                     /translation="MTALDTLARRCGIALSYPDGRGGEHRVGPDTLQAILGALGIEAA
                     TEERAGESLAELERHEWRGALPPVVVARDSAVAVELTLPAATRTVTWHLRAEDGSQRT
                     GEVAFGDLELVARNDGDARPGRRKERRRLKLGNVALGYHELSVEPQSASSVVIVTPGR
                     CHLPSVDDARGLWGLALQLYLLRSERNFGIGDFGDLNALAQELTQRGCGMLGLNPLHA
                     PFPDDPEHASPYSPASRLLLNPLNIDVAALVEGHESPAAALLRDAGFVAELDGCRAAA
                     TVQYAKVAALKQRVLRAAYADARARGGASAGEFASFRAAQGAAFERHCLYFALRAHML
                     AEGLGPGDWHRWPQPYQDSRAAVVARFARTHADEITWVAWQQWLADRQLAAAAQQARD
                     MPVGLYRDLAVGCDAAGAETWAESELLVEGLRIGAPPDPFSATGQEWGLPAPHPQRMC
                     ETGYRAFVELLRANMRHAGALRIDHVMALQRLYVIPEGAPASAGAYLSYPREDLVGIL
                     ALESARQRCVIVGEDLGVVPEGFRERMTEANVLSYRVLRWQRDGQRMFRPHEYPRLAI
                     AVVGNHDLATLRAWWEGADLTLEHEHGGLPESELAGARQARDADRHSLLELLRAEGLL
                     AGGDAQLSYEALFSAVH"
     gene            complement(1921..>2714)
                     /locus_tag="VM692_02730"
     CDS             complement(1921..>2714)
                     /locus_tag="VM692_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HUQ51108.1"
                     /translation="FTLHDLVAYNERHNEANGEDNRDGHSDNRSWNCGAEGPTDDAAV
                     NELRGRQMRNILATLLLSQGTPMLLAGDEFARTQRGNNNAYCQDDEISWVDWTLAEAN
                     LPQVRFVQSLTALRRRYPILRRNRFLSTVVNEAIGVKEVTWISVSGAEMQEGDWNGGS
                     LACFGMLLDGRAQATGIKQRGQDATMLLIVNSHHDPVDFKLPAAGDDLAVARWSLLLD
                     TQFPMPAHTAHNEYTAGDVYTVTARSLLLLKLELPPEAPLWSEQR"
BASE COUNT          416 a          956 c          932 g          410 t
ORIGIN      
        1 cgtgcacggc cgagaacaac gcctcatagc tgagctgcgc gtcgccgccg gccagcaaac
       61 cctcggcgcg cagcaactcg agcagcgaat gccggtcggc gtcgcgcgcc tgccgggcgc
      121 cggccagctc ggactccggc aggcctccgt gctcgtgctc gagcgtgagg tcggcgcctt
      181 cccaccaagc gcgcaaggta gcaaggtcgt gattcccgac cacggcgatc gcgaggcgcg
      241 ggtactcgtg cgggcggaac atccgctgcc cgtcgcgttg ccagcgcagc acgcgatacg
      301 acagcacgtt cgcctccgtc attcgctcgc gaaacccttc gggcacaacc cctaagtcct
      361 cgccgacgat gacgcagcgt tgtcgcgcgc tttccagcgc tagaattccg acgaggtcct
      421 cgcgcggata ggagagataa gcgccggccg aggccggggc gccttcagga atcacataca
      481 gccgttgcag cgccatgaca tgatcgatgc gcagcgctcc cgcgtggcgc atgttggcgc
      541 gcagcagctc gacgaacgca cggtacccgg tctcgcacat acgctgcggg tgcggcgccg
      601 gcagacccca ctcctggccc gtggccgaga acggatccgg cggcgcgccg atgcgcaggc
      661 cttcgacgag cagctcgctt tcggcccagg tctcggcgcc ggccgcgtcg caaccgacgg
      721 cgaggtcgcg atacaagccg accggcatgt cgcgcgcttg ctgtgccgcc gccgcgagct
      781 gccggtccgc gagccactgt tgccacgcga cccaggtaat ttcgtcggca tgcgtgcgcg
      841 cgaaccgcgc taccacggct gcgcgcgagt cctggtacgg ctgcggccag cggtgccaat
      901 cgcctgggcc caaaccttcg gccaacatgt gtgcgcgcag cgcgaagtag aggcagtgac
      961 gctcgaacgc ggcgccttgc gccgcgcgaa agctcgcgaa ctcgccggcg gacgcgcctc
     1021 cgcgagcacg cgcgtcggcg tacgcggcac gcaagacgcg ctgcttcaac gcagcgactt
     1081 tggcgtactg gaccgtcgcg gcggcgcggc agccgtcgag ctcggccacg aaacccgcgt
     1141 cgcgcagcag cgccgcggcc ggcgactcgt gcccctcgac cagggccgca acgtcgatgt
     1201 tcaacgggtt aaggagcagc cggctcgccg gagagtacgg gctcgcgtgc tccgggtcat
     1261 ccggaaacgg cgcatgcagc ggattcaagc ccagcatgcc gcagccgcgc tgcgtgagct
     1321 cctgcgccag cgcgttgaga tcgccaaagt cgccgatgcc gaagttgcgc tcggagcgca
     1381 gcagatagag ctgcagcgcg agaccccata gaccgcgggc gtcgtccacg ctcgggagat
     1441 ggcaacggcc cggcgtcacg atgacgacac tgctggcgct ctgcggctcg acgctcagct
     1501 cgtgatagcc gagcgcaaca ttgccgagtt tcaaccggcg gcgttccttg cggcgcccag
     1561 ggcgcgcgtc gccgtcgttg cgcgcgacga gctcgagatc gccgaatgca acctcgcccg
     1621 tgcgctgcga gccgtcctcg gctcgcaggt gccaggtgac ggtgcgcgtc gcagcgggca
     1681 gcgtcagctc gaccgcgacg gcggagtcgc gcgcgacgac gacaggcggc agcgcacctc
     1741 gccattcgtg ccgctcgagc tcggccagcg actcgcctgc gcgctcctcc gttgcggcct
     1801 cgatcccgag cgcgccgaga atcgcttgca gcgtgtccgg cccgacgcga tgctcgccgc
     1861 cgcggccgtc cggatacgac agagcgatgc cgcagcggcg cgccaatgtg tcgagcgccg
     1921 tcatcgctgc tcgctccaga gcggcgcctc gggcggcagc tccagcttca agagcagcag
     1981 cgagcgcgcc gtcacggtgt agacgtcacc ggccgtgtac tcgttgtggg ccgtgtgcgc
     2041 cggcatcggg aattgcgtgt cgagcagcag cgaccagcgc gcgaccgcga ggtcgtcacc
     2101 ggcggccggc agcttgaaat cgacggggtc gtggtgcgag ttgacgatca acagcatcgt
     2161 cgcgtcctgg ccgcgctgtt tgatgccggt ggcctgagcg cggccgtcga gcagcatgcc
     2221 gaagcaagcg agcgagccgc cgttccagtc gccctcttgc atctcggcgc cgctgacgct
     2281 gatccacgta acctctttga cgccgatggc ttcgttgacg actgtcgaca gaaagcggtt
     2341 gcggcgcagg atcggatagc ggcgccgcag cgccgtgagg ctttgcacga accgcacttg
     2401 cggcagattc gcctcggcga gcgtccagtc cacccagctg atctcgtcgt cctgacaata
     2461 ggcgttgttg ttgccgcgct gcgtgcgcgc gaactcgtcg ccggcgagca gcatcggcgt
     2521 gccttgcgac agcagcaggg tcgccaggat gttgcgcatt tgccggccgc ggagctcgtt
     2581 gaccgccgcg tcgtcggtcg gcccttcggc gccgcaattc cacgaacggt tgtccgaatg
     2641 cccgtcgcgg ttgtcctcgc cgttcgcctc gttgtggcgc tcgttgtagg ccacgaggtc
     2701 gtgcagcgtg aagc
//
LOCUS       DATLEE010000127         2753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9758667, whole genome shotgun sequence.
ACCESSION   DATLEE010000127 DATLEE010000000
VERSION     DATLEE010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9758667"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VM692_02735"
     CDS             complement(<1..418)
                     /locus_tag="VM692_02735"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HUQ51109.1"
                     /translation="MVVEGPIGVGKTSLAKRLAKSFASELILEQAEENPFLERFYRNR
                     RQSALPTQLFFLFQRARQIEQIRQSDMFSPVRIADFLLQKDRLFAEINLDAHELGLYQ
                     QVADTLELDPPTPDLVVYLQAPAPVLLKRVATRGIPY"
     gene            complement(528..1856)
                     /gene="pcnB"
                     /locus_tag="VM692_02740"
     CDS             complement(528..1856)
                     /gene="pcnB"
                     /locus_tag="VM692_02740"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HUQ51110.1"
                     /translation="MLNDTELESRHGPVIVPRSEHNVSRANISPNALKVLYRLKSAGF
                     EAFLVGGGVRDLLLDRHPKDFDVVTNADPDQIQDVFRNCRLIGRRFRLAHVRFGRDIV
                     EVATFRATGVEAADDDRAHSDSGRILRDNVYGTIGEDVWRRDFTVNALYYNITDFSIW
                     DYTNGVADVRSRTLRLIGDPEKRYREDPVRMLRAVRFAAKLDFQLAPETAAPIASLGG
                     SLKDVPSARLFDELLKLFQSGHAVRSFELLLQYDLLRYLFRDTQAQLETGDRDKIVDF
                     IRRGLKNTDDRVRDDKPVTPMFLYALFLWFPIQALARRLESEGWNDGQAMLEASQRIV
                     ASQQTAFPRRFSSPMKELLNMQWRFGQRHGARAARLLQHKRFRAAYDFLVLRASCGEV
                     DQETVDWWTEIQNLPPEEQSKRLNLTKQGRRRRGGRRRGGRQNEMNDVVV"
     gene            1925..2000
                     /locus_tag="VM692_02745"
     tRNA            1925..2000
                     /locus_tag="VM692_02745"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1959..1961,aa:Glu,seq:ctc)
BASE COUNT          487 a          914 c          861 g          491 t
ORIGIN      
        1 cgtacgggat gccgcgcgtc gcgacgcgct tcagcaaaac cggcgccggc gcctgcagat
       61 aaacgacgag atccggcgtc ggcggatcga gctcgagcgt gtcggctacc tgctggtata
      121 ggccgagctc gtgcgcatcg agattgatct ccgcaaacaa ccggtctttt tgcagcaaga
      181 aatccgcgat acgcaccggc gagaacatgt cgctctggcg aatctgctcg atctgccgcg
      241 cgcgctggaa caagaagaac aactgcgtcg gcagcgccga ctgacggcgg ttgcggtaga
      301 accgctcgag gaacgggttc tcctcggctt gctcgaggat cagctcgctg gcgaagcttt
      361 tcgccaaacg tttcgcgagg ctcgtcttgc cgacgccgat cggcccttca acgacgatgt
      421 aacgcggccc ttcgagcttc gtcgcgtcgt cgctcggctc acctttcgca ctgctgcggc
      481 cgaccgtgtc cgctgcgcgc ttcggccgct tctctgctga cgtcgcgcta aacgacaacg
      541 tcgttcatct cgttctggcg cccgccgcga cgccgtccgc cgcgacggcg gcggccttgc
      601 ttagtcaagt tcagccgctt gctctgctct tcgggcggca gattctgtat ttccgtccac
      661 caatcgacgg tttcctgatc cacttcgcca cagctcgcac gcagcacgag gaagtcgtaa
      721 gcagcgcgaa agcgcttgtg ttgcaacaag cgcgcggctc gggcgccgtg ccgctgcccg
      781 aaacgccact gcatgttcaa aagctctttc atcggcgagc tgaaacggcg cggaaacgcc
      841 gtttgctgcg acgcgacgat gcgttggctc gcctcgagca tcgcctgacc gtcgttccag
      901 ccctcgcttt ccaagcggcg cgcgagcgcc tgtattggga accacaggaa caacgcatac
      961 aagaacatcg gcgtgacggg cttgtcgtca cgcacgcgat cgtcggtgtt cttgagaccg
     1021 cggcggatga agtcgacgat cttgtcgcgg tcccccgtct cgagctgcgc ttgcgtgtcg
     1081 cgaaacagat agcgcagcaa atcgtattgc aagagcagct cgaagctgcg gacggcgtgg
     1141 ccgctctgga acagcttcaa gagctcgtcg aacaagcgcg ccgacggcac gtccttcagc
     1201 gacccaccga gcgacgcgat cggcgcggca gtctcgggcg cgagctgaaa atcgagcttc
     1261 gccgcgaaac gcacggcgcg cagcatgcgc acggggtctt cgcgatagcg cttttccggg
     1321 tcaccgatca gccgcaacgt gcgcgagcgc acgtcggcca cgccgttcgt gtaatcccag
     1381 atactgaagt ctgtgatgtt gtagtacagc gcgttgaccg tgaagtcgcg acgccaaacg
     1441 tcttcgccga tcgtgccgta gacgttgtcg cgcagaatgc ggccactgtc ggaatgcgcg
     1501 cggtcgtcgt cggccgcctc gacgcccgtc gcgcgaaacg tcgccacttc gacgatgtcg
     1561 cgcccgaagc ggacatgcgc gaggcgaaag cgccggccga tcagtcggca gttgcgaaag
     1621 acgtcctgga tctgatcggg atcggcgttc gtgacgacgt cgaagtcctt gggatggcga
     1681 tccagcaaca ggtcgcgcac gccgccgccg acgagaaacg cctcgaaacc cgcgctcttc
     1741 aatcggtaca agactttgag agcgttgggc gagatgttgg cccggctgac gttgtgttcg
     1801 gagcgcggga cgatgacagg accgtgacgc gactctaatt ccgtgtcgtt caacaagccg
     1861 ccgtggtttc gccctgcgag aaaggccggt atcataccag ccctcgctca gcgcgggcag
     1921 ccgcgctccc ttcgtctagc ggtccaggac gtggccctct caaggctaaa acgggggttc
     1981 gattccccca gggagcgcca tttctttcaa cgggttgctg cgccgcccgc gcaactaccc
     2041 ggcagcagac atgcccgcaa gcagggagcg aggcccttcg gacaggcgcc tcgagcaaga
     2101 atcagcggcc ctccgggaac cccgcgaggc gcgcggcaag ttgttacggg gatggagcag
     2161 gcggcgggcc ggccttccgc ttgatctccg cgcgctcgaa atgcgcttga gcacgcggtg
     2221 cggctgcgcc ccctctgcga tcgggtgaga gctccttgat cggcttccac gaacgccaaa
     2281 cggcgacgac cgcggccgtg accgcaagtg tgcccgttac accgagaacc gcaccgaaga
     2341 acagccaagc cacaggtttt ccatccttgg aaaactgcca agctaatggc gctccctgaa
     2401 cgatctgtga acgttgcgcc cttccctacg ccgtcacttc cttcgaaacg acgcttccga
     2461 cgatgcggac ctgctgcgcc gctttgcgct cgagcgttag cagcggggag tgtcggtcgt
     2521 cgatttggcc ctacgtctcg gcgccggccg ccatagccgc cctcagcgcc gcgcgggctt
     2581 cctcgaggtc ttggcgagtg cgcgcctctt ttgccaagag ctccggcctt gccccgtcgc
     2641 cgctcatatg ggcttcggtc aggggcctga cgcagtcctg gtgagacagg cacccggcgg
     2701 cgcccgccca aagcccgcgt ggacacccgc gaaccgcacg ccagccttga gag
//
LOCUS       DATLEE010000128        10808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7522817, whole genome shotgun sequence.
ACCESSION   DATLEE010000128 DATLEE010000000
VERSION     DATLEE010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7522817"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VM692_02750"
     CDS             complement(<1..352)
                     /locus_tag="VM692_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HUQ51111.1"
                     /translation="MKKALLHSAVIAALTAGTAQVSWSQEGLEEIVVTATRREQNLQD
                     VPLAVVAYTGAMLERQGIENMEDLKAVVPNLVVAGNLGGSNTASFTIRGIPNVGTYID
                     GIWQVSNNGLLLREF"
     gene            666..1229
                     /locus_tag="VM692_02755"
     CDS             666..1229
                     /locus_tag="VM692_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51112.1"
                     /translation="MNRKPRLPLALLAVSSAALGTFAVIRSSTATAADADYSPAISVD
                     EIMESMVMPSAQQLWDAVGVDVSEKGSIEKKPETDEQWAQLRAAAVTLVEATNALVVP
                     GRHAAPASKPASPEGSGELGAAEIEALLAKERPAWVAHAQVLRATAMQALAAVDARDT
                     DKISEVGGAIDEACESCHLQFWYPDQK"
     gene            complement(1278..2678)
                     /locus_tag="VM692_02760"
     CDS             complement(1278..2678)
                     /locus_tag="VM692_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUQ51113.1"
                     /translation="MSRPERAVAADNASDKEVTELVAKARKAQAIYEGFSQEQVDAIV
                     RDMAKYVYDNAEQLARMAVDETGIGNYEDKVLKKKGKARVIWNNLKGKKSRGVIGEDP
                     ENNLVFVAKPMGIVAAVCPVTNPIATPMCNGMFALKTGNAVIFAPHPKAQKCTDHLTN
                     EFMKIVKKHGGPDNLIQTIKKGSVEKTQELMRSVDVVVATGGGAMVKSAYSSGKPSYG
                     VGAGNMPVIIDRGVDFKDAVGKIVTGAAFDNGIICSHEQFVLTPEEKYDETVKLFKDT
                     GKVWFTSDEAQIQKLRELVFPGGHLNKDVVGRSPREIGAMVGIDVPASARIILLPAKG
                     AGTADVLAKEKLCPVVAILPYTTFNDAVAKAKANLLVEGAGHSAALHSNDEKNIREAG
                     VELPISRLVVNQPSALTAGGSLTNGFAPTTTLGCGSWGGNSISENLDYKHLMNVSRIG
                     KVITNKKVPTDEEIFA"
     gene            2794..3582
                     /locus_tag="VM692_02765"
     CDS             2794..3582
                     /locus_tag="VM692_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HUQ51114.1"
                     /translation="MRSGYWQDLTTTEFGRVDGERTVALLPVAAVEQHGPHLPLATDA
                     LINDGIVRAALERLPADTTLLVLPAQNVGLSPEHTSFAGTLSVRDTSLLDVWTDIGRS
                     VARAGLRKLVVFNTHGGQKTLVDLLAVRLRSELGMLVVRATYFAFGALPGLFDPAEVM
                     HDIHGGEIETSLLLHLRPDLVRTAALADFKGLPHELGSRNQLLGAEKPIGFGWRAEDL
                     NPAGACGNAARADGRRGAQHLSYLADRLAGLVGEVAATPLTILR"
     gene            3591..4574
                     /locus_tag="VM692_02770"
     CDS             3591..4574
                     /locus_tag="VM692_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51115.1"
                     /translation="MARRPQAPRATVDEQLAALDAVPSERGAKAEALQRALSERHYSV
                     VAKAARLAEDALVYELVPTMIAAYPRFLDKPLKSDPNCFAKKALARALVALDCGDTEL
                     FRRGLAYRQLEPVWGGTADTAVDVRTSCAMGLVASGYSRALVELTALLADGEAGARLG
                     AVRAIACGNPREAELLLRAKALAGDAEPQVLGECFRGLLGVEPEESLGFVAGYLEHAD
                     EAVRELAALALGESRIDGALAPLKQAWNGVLVGNELRRALLRAAAAHRSEAAFDWLLS
                     LAGEGRAAVAADVIEALAPYKHNAKLMQRLASTLAERGDAALLERLGALAS"
     gene            4576..5109
                     /locus_tag="VM692_02775"
     CDS             4576..5109
                     /locus_tag="VM692_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ51116.1"
                     /translation="MAERAFAVRRAAAEDAAELARLAEVTFAATFGHLYPPEDLASYV
                     AATYTEEACLQVLADERMACWLVGAEGEPAVGFALAGYCKLPVAGLEPTAGELRQLYV
                     RSTHQGRQLGTQLFDAALAWLERHYSPLYIGVWSLNHGAQRFYGRYGFVKVGEYGFAV
                     GKTIDREFILRRSARGR"
     gene            5172..6677
                     /locus_tag="VM692_02780"
     CDS             5172..6677
                     /locus_tag="VM692_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ51117.1"
                     /translation="MNDKYRLRQLALVGVALAAHAAYGQGQSAREVLEQAAQAMGGLQ
                     RLQSLDNLVMTGFGQYVNQQGGSALSPDARAPLKWTVAHDAQRVFDLKNERALNLDRR
                     GSLFPFAIERPWDRTSQVQTGVAALDHPVPALLEALDADTKLGPVSVEDGLTVVEFTL
                     AQGPTIWLAVDPATKLPAWVRSIGPSTTLGDITTTTYFTGYLPFGDLELPVGLTAEMD
                     WRHVTAMMFNVDSYRANATNLPELPAAPAPAAPAEPRRAEATKIADKVWDVRIGGNGG
                     AVIEFADHLVMFEAYNNESDTFARLDVADALVPGKKVTHVIVTHHHFDHSGGLRAAVS
                     RGLTIIARRGNEGIFREMVARPAPNFPDALARNPQPLKFVPVDEQLTLDDGTMRVDVY
                     RAVGHLHMAESVFAYIPGPRIFLEGDFTTFDWDWNWWGGAYLDNVERYGLSPAINIPV
                     HGIVTPYDETIATIEKQVRNARDYCTRQARAGVYPAGCPVQYSRDASRAPR"
     gene            6687..7745
                     /locus_tag="VM692_02785"
     CDS             6687..7745
                     /locus_tag="VM692_02785"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HUQ51118.1"
                     /translation="MPKLAARQALNRLLGAELPLIQAPMAGVQASALAVAVANAGALG
                     SLPCAMLGVETMRNEVAAIRAGTDRPYNVNFFCHAPQSPSAEREAAWRRALAPYFREY
                     GIEPDSIDAAPARMPFTAASAAALEEFRPRVVSFHFGLPAADLIARVKSWGSKVVASA
                     TTVAEARWLEANGADAIVAQGAEAGGHRGMFLTTDVGTQIGTFALVPQIAKAVRVPVI
                     AAGGIADARGVAAAMALGAAGVQVGTAYLLCHEATTSAVHRAALQSDAARETALTNVF
                     TGRPARGIVNRLMRELGPISALAPAFPLAAAAIGALRARAEPRGCGDFSPLWAGQNVG
                     GCKEIAAAELTRELAEGL"
     gene            complement(7750..8871)
                     /gene="ribA"
                     /locus_tag="VM692_02790"
     CDS             complement(7750..8871)
                     /gene="ribA"
                     /locus_tag="VM692_02790"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HUQ51119.1"
                     /translation="MSSPTTQEINGNPAALTFDRAVDELRRGRAVQVTDGARGLLVAA
                     IETLQPPLFGRLVAAADGRAVMLVTAERAHAAALSRRLSGAVAVAFPRETDLECLRTL
                     AGVDGIAPVDDEAFVVDPAPRNAPLAAAAFQLAKAGRLVPALIGFDTDGDAVAGVLTI
                     AVSDVAHHAPRFGHHALQLLSRARVPLADAVECEIAVFRDEHSLAEHVAVVIGQPDAA
                     SAVPVRLHSACLTGDLLGSLRCDCGEQLRTAVARIAALGGGVLLYLDQEGRGIGLPNK
                     LRAYVLQDGGLDTVDADQHLGFLADERSYDVAAALLTELGYTRIRLLTNNPQKINSLR
                     DHGIEVVGRLPLVTTSNTHNERYLRAKHERAGHLDIAEK"
     gene            8956..9852
                     /locus_tag="VM692_02795"
     CDS             8956..9852
                     /locus_tag="VM692_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HUQ51120.1"
                     /translation="MKAKTNESADLAALEAVAWQSLLSAAKAARQGRELDDEAGGLLV
                     RSAREFYASLDSPATIRPLLELYAPLCAAGPLTFAHLGQSLDGQIATASGDSYYVTGP
                     DNVRHLHRLRALADAIVVGAGTVARDDPQLTVRHVAGANPVRVVLDPSARLDPDRRVF
                     RDGAAPTLVVHAEGVGSRAPGNAEVLRVPHAGGRLALDALLARLHERGLSSVFVEGGG
                     ETVSRFVESGLIDRLHVAIAPLLTGRGRPGLTLAARDKIADCVRPAHRVFAMGGDVLF
                     DCDLRAAAPAVSEPSGSLTRVS"
     gene            complement(9862..>10808)
                     /locus_tag="VM692_02800"
     CDS             complement(9862..>10808)
                     /locus_tag="VM692_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HUQ51121.1"
                     /translation="ASVTIISLTRSPPEELSPTTGGEPSAQVDAAVIDTTAAAAATPT
                     TAATPDLPTSVELIETSDADVIPLVRHNPHYPPAALEQKAGGYVQLKFDITTAGAVEN
                     VSVVKSDDAQFEQSAIDALSKWRYLPRLVAGKRVRRDGVQTIIRFTMEGAENPAAEPT
                     PRQQEELEAVMRNELAYLAGLEVALDRLAADNLRGVELQLDEMQAVYGADRLDLWNFY
                     GYLYTVEGNYARAIDAYETGVAVAMRSPYPTTGPYIPLAKLYFARHQYDLALKTLLRP
                     QQVVSNGRPLGGARRDEAAALIERLRALGVTEETLIGR"
BASE COUNT         1646 a         3590 c         3691 g         1881 t
ORIGIN      
        1 cgaactcgcg cagcagcaag ccgttgttcg atacctgcca aatgccgtcg atataggtgc
       61 ctacgttggg aatcccgcgg attgtgaacg atgcagtatt ggaaccgccc aagttgccgg
      121 cgacgaccag gttcggcacg acggccttga ggtcctccat gttctcgatg ccctgacgct
      181 cgagcatcgc gcccgtgtag gcaacgacgg ccagcggcac gtcttgcagg ttttgctcgc
      241 gacgggtggc ggtgacgacg atttcctcga ggccctcttg tgaccaagag acctgggccg
      301 tcccggccgt taatgcggct atgacagcgg agtgcaataa cgccttcttc atgatgtccc
      361 ccttcgtggg cgagtcgccg atcgacccac cgctggtaga gcgggcattg tgtgtcgaat
      421 acaaccgcaa aactagcccc tgaacagttt tcggagatag cacgcgacgg gagcaaacgg
      481 cacgcgccga cctccgtaac cgaggaattg tcttgggcgt aagctttggt gtcgcgtccg
      541 tgccacaacg gcgtgattcg gcaatcagat tgcgccgcat tacggatttt taatgtggcg
      601 ctcacctttt gcagcgttca cgcggcgcat gctggagcgc atcacacgat cgcaagggag
      661 tgatcatgaa tcgcaagccc cgtctgcccc tcgcgctact cgcggtatcg tccgcggcgc
      721 tcgggacgtt cgctgtcatc cgctcgtcca cggcgacggc ggccgatgcg gactattcgc
      781 ccgcaatctc ggtcgacgag atcatggagt cgatggtcat gccgtcggcc cagcagctct
      841 gggacgccgt cggtgtcgac gtgtccgaga aggggtcgat cgagaagaag cccgagaccg
      901 acgagcaatg ggcgcagctg cgggcggcgg ccgtcacgct cgttgaagcg acgaacgcgc
      961 tcgtcgtacc gggccggcat gcggcaccgg cgagcaagcc cgcgtccccg gaaggcagcg
     1021 gcgagctcgg ggcggcggag atcgaagcgc tactcgcaaa agagcgcccg gcatgggttg
     1081 cgcatgcgca agtgttgcgc gcgaccgcga tgcaagcgct cgcggcagtc gacgctcgcg
     1141 acaccgacaa gatctccgag gtcggcggcg cgatcgacga agcctgcgag tcgtgtcacc
     1201 tgcagttctg gtatccggac cagaaatgac gacgcgcgcc tgagcgcgcg tcgtgcgagc
     1261 cgcggaaagc gcctgcgtta ggcgaagatt tcctcgtcgg tcggcacctt cttgttcgtg
     1321 atgaccttgc cgatgcgcga cacattcatc aggtgcttgt agtcgaggtt ctccgagatc
     1381 gagttgccgc cccacgagcc gcagccgagc gtcgtcgtcg gcgcgaagcc gttcgtcaac
     1441 gagccgcccg cggtcagcgc gctcggctga ttgacgacga gccggctgat cggcagctcg
     1501 acgccggcct cgcgaatgtt cttctcgtcg ttcgagtgca gcgccgccga atggccggcg
     1561 ccctcgacga gcagattcgc cttggccttt gcgacggcgt cgttgaacgt cgtgtacggc
     1621 aggatcgcga cgacggggca gagcttctct ttcgcgagca cgtcggccgt gccggcgcct
     1681 ttcgcgggca acaggatgat gcgcgcgctc gccggcacgt cgatgccgac catcgcgccg
     1741 atctcgcgcg gcgacctgcc gacgacgtcc ttgttcaggt ggccgcccgg aaacacgagc
     1801 tcgcgcagct tctggatctg cgcttcgtcg ctcgtgaacc acaccttgcc ggtgtccttg
     1861 aacagcttga ccgtctcgtc gtacttctcc tcgggcgtca gcacgaactg ctcgtgcgag
     1921 cagatgatgc cgttgtcgaa cgcggcgccg gtcacgatct tgccgacggc gtctttgaaa
     1981 tcgacgccgc ggtcgatgat gacgggcatg ttgcccgcgc cgacgccgta ggacggcttg
     2041 cccgacgaat acgccgactt gaccatcgcg ccgccgccgg tcgcgacgac gacgtcgacg
     2101 gaacgcatga gctcctgcgt cttctcgacg gaacccttct tgatcgtttg aatcaagttg
     2161 tcgggaccgc cgtgcttctt gacgatcttc atgaactcgt tcgtcagatg atcggtgcac
     2221 ttctgcgctt tcggatgcgg tgcgaagatc acggcgttgc ccgtcttcag cgcgaacatg
     2281 ccgttgcaca tcggcgtcgc gatcgggttc gtgacggggc agaccgcagc gacgatgccc
     2341 atcggcttgg cgacgaacac gagattgttc tcggggtctt cgccgatgac gccgcgcgac
     2401 ttcttgccct tcaagttgtt ccagatgacc cgggccttgc ccttcttctt caggaccttg
     2461 tcctcgtagt tgccgatgcc cgtctcgtcg acggccatac gcgcgagctg ctcggcgttg
     2521 tcatagacgt acttcgccat gtcgcgcacg atcgcgtcga cttgctcttg cgagaatcct
     2581 tcgtagatcg cctgcgcctt gcgggctttg gccaccagct cggttacttc cttatcactg
     2641 gcgttatcgg cggcaactgc gcgctcgggt ctgctcataa agtgctcctg cacagggctg
     2701 atgcgggggt caagcccagt attatccaag gttgacgccg cttccggcaa cccggcacga
     2761 tgccgcgcag tgcttggttc aggtgccctc agcgtgcgca gcggctactg gcaagacctc
     2821 acgaccacgg agttcggccg cgtcgacggc gagcgcaccg tcgcgctgct gcccgttgcc
     2881 gccgtggagc agcacggccc gcatttgccg ctcgcgaccg acgccctcat caacgacggc
     2941 atcgttcggg ccgcgctcga acggttgccg gcggacacaa cgctgttggt gctgcccgcg
     3001 caaaacgtcg gcttgagccc cgagcacacg agctttgccg gaaccctctc cgtgcgcgac
     3061 acctcgctgc tggacgtctg gacggacatc ggccgcagcg tggcgcgcgc ggggctgcgc
     3121 aagctcgtcg tcttcaacac gcacggcggt cagaagacgc tcgtcgatct cttggccgta
     3181 cggctgcgct ccgagctcgg catgctagtc gtgcgtgcga cgtatttcgc gttcggcgcg
     3241 ttgccggggt tgttcgatcc cgccgaagtc atgcacgaca tccatggcgg cgagatcgag
     3301 acgtcgctgc tgctgcactt gcggcccgat ctcgtgcgca cggcggcgct cgccgacttc
     3361 aaaggcttgc cgcacgagct gggtagccgc aaccaattgc tcggtgccga gaagcccatc
     3421 ggctttggtt ggcgagccga agatctgaac ccggccggcg cttgcggcaa cgcggcgcga
     3481 gccgacggcc gccgcggcgc acaacacttg tcgtacttgg ccgatcggct cgcgggcctc
     3541 gtcggcgagg tcgccgcgac gccgctgacg attctgcgtt gatcccgctc gtggcgcggc
     3601 gaccgcaagc tccccgcgcg accgtcgacg agcagctcgc ggcgctcgat gccgtgccga
     3661 gcgagcgcgg tgccaaggcc gaggcgctgc agcgcgcttt gtcggagcgt cactacagcg
     3721 tcgtcgcgaa agccgcgcgc ctcgccgagg acgcgttggt ctacgagctc gtgccgacaa
     3781 tgatcgcggc gtatccgcgc ttcctcgaca agccgctgaa gtccgatccc aactgtttcg
     3841 ccaagaaggc gcttgcacgc gctctcgtcg ctctcgattg cggcgacacc gagctctttc
     3901 gtcgtgggct cgcgtatcgg cagctcgagc cggtctgggg cggcacggcc gatacggccg
     3961 tcgacgtgcg cacgagctgc gccatggggc tcgtcgcatc gggttactcg cgtgcgctcg
     4021 tcgagctgac ggcgctgctc gccgacggcg aggccggcgc gcggctcggc gccgtgcgcg
     4081 cgattgcctg cggcaatcca cgcgaagcgg agctgctgtt gcgcgccaag gcgctcgccg
     4141 gcgatgccga gccgcaggtg ctcggcgagt gtttcagagg actgcttggc gtcgagcccg
     4201 aggagtcgct cgggttcgtc gccggctatc tcgagcacgc cgacgaagcc gtgcgcgagc
     4261 tggccgcgtt ggcgctcgga gagtcgcgca tcgacggcgc gctcgcgccg ctcaagcaag
     4321 cttggaacgg cgtgctcgtc ggcaacgagc tgcggcgtgc gttgctgcgc gccgccgcgg
     4381 cgcacaggag cgaggccgcc ttcgactggc tgttgtcgct cgccggggaa gggcgtgcgg
     4441 ccgtcgcggc cgacgtcatc gaggcactgg cgccgtacaa gcacaacgcc aagctgatgc
     4501 aacggctcgc gagcacgttg gccgagcgcg gcgacgccgc tttgctcgag cgcctcggcg
     4561 cgctcgcttc ctgacgtggc cgagcgagcc ttcgcagtcc gccgcgctgc cgccgaagac
     4621 gccgcggagc ttgcgcgcct cgccgaggtc acgttcgccg caacgttcgg gcacctgtat
     4681 ccgcccgaag atctcgcgag ctacgtcgcg gccacctaca ccgaagaggc gtgcctgcaa
     4741 gtcttggcgg acgagcgcat ggcttgctgg ctcgtgggcg cggagggcga gcctgccgtg
     4801 ggattcgcgc tcgccggcta ctgcaagctg cccgttgccg gtctcgagcc gacggccggc
     4861 gagctgcgcc agctctacgt tcgctcgacg catcagggcc gacagctcgg cacgcagctg
     4921 ttcgacgcgg cgctcgcgtg gctcgagcgg cactattcgc cgctctatat cggcgtctgg
     4981 tctttgaacc atggtgcgca gcgcttctat ggccgctacg gcttcgtaaa ggtcggcgag
     5041 tatggttttg ccgtcggcaa gacgatcgac cgcgagttca tcctgcgccg cagcgctcgg
     5101 gggcgttgac gccgcgagcg tttgcgcacg acgctagcgg ctgttacgtg gaatcgcggc
     5161 aggaggggag catgaacgac aagtatcggc tgcggcagct cgcgctcgtc ggcgtcgcgc
     5221 tggcggcgca tgcggcctac ggacaaggac aatcggcgcg cgaggtgctc gagcaggcgg
     5281 cacaggcgat gggcgggtta cagcgcctgc aaagcctcga caatctcgtc atgacgggct
     5341 tcggccagta cgtcaatcaa caaggcggca gcgcgctgtc gccggacgcg cgcgcaccgc
     5401 tgaagtggac cgtcgcgcac gacgcgcagc gcgtgttcga tctgaaaaac gagcgcgcgt
     5461 tgaatctcga tcgtcgaggc tcgctgttcc cgttcgcgat cgagcgcccg tgggaccgga
     5521 cgagtcaggt gcagaccggc gtcgcggcgc tcgatcatcc ggtgccggcg ctgctcgagg
     5581 cgctcgatgc cgacacgaag ctcgggcctg tcagcgtcga ggacgggcta accgtcgtcg
     5641 agttcacgct ggcgcagggt ccgacgattt ggctcgccgt cgatcccgcg acgaagctgc
     5701 cggcttgggt gcgctcgatc ggccctagca cgacgctcgg cgacatcacg acgacgacgt
     5761 actttacggg ctacttgccg ttcggcgact tggagttgcc cgtcgggctc acggccgaga
     5821 tggactggcg ccacgtgacg gcgatgatgt tcaacgtcga ctcgtatcgc gccaatgcga
     5881 cgaacttgcc ggagctcccg gcagcgcccg cgcccgccgc gccggcagag ccgcgccggg
     5941 ctgaggccac gaagatcgcc gacaaggtct gggacgtgcg catcggcggc aacggcggcg
     6001 ccgtcatcga gttcgccgat cacctcgtca tgttcgaggc gtacaacaac gagtcggata
     6061 cctttgcgcg gctcgacgtc gccgacgcgc tagtgccggg caagaaagtc acgcacgtca
     6121 tcgtcacgca ccatcacttc gatcattccg gcggattgcg ggctgccgtc tcgcgcggtc
     6181 tgacgatcat cgcgcgccgc ggcaacgaag gcattttccg cgagatggtc gcgcggcccg
     6241 cgccgaattt tcccgacgcg ctcgcgcgca atcctcagcc gttgaagttc gtgcccgtcg
     6301 acgagcagct cacgctcgac gacggcacga tgcgcgtcga cgtgtatcgc gccgtcggtc
     6361 atctgcacat ggccgaaagc gtgttcgcgt acatcccagg gccgcggatc ttcctcgagg
     6421 gcgacttcac gaccttcgac tgggactgga actggtgggg cggggcttat ctcgacaacg
     6481 tcgaacgtta tggcctgagc cccgcgatca acatccccgt gcacggcatc gtgaccccgt
     6541 acgacgagac gatcgcgacg atcgagaagc aagtgcgaaa cgcgcgcgac tattgcacgc
     6601 ggcaggcgcg ggccggcgtc tatccggcag ggtgcccggt ccagtactcg cgcgatgcga
     6661 gccgcgcgcc gcgctagggt tcgtgcatgc cgaaactcgc cgcgcgtcag gcgctcaacc
     6721 gcttgctcgg cgccgagctg ccgctgattc aggcgccgat ggccggcgtg caggcgagtg
     6781 cgctggcagt ggcggttgcg aatgccggcg cgctcgggtc gctgccttgc gcaatgctcg
     6841 gcgtcgagac gatgcgcaac gaggtcgcgg cgatccgcgc cggcaccgac aggccctaca
     6901 acgtcaattt cttctgccac gcgccgcaga gcccgagcgc cgagcgcgaa gcggcgtggc
     6961 ggcgcgcatt ggcgccgtac ttccgcgagt acggcatcga gccggactcg atcgacgctg
     7021 ctccagcacg catgccgttc acggccgcgt cggcggctgc gctcgaggaa ttccgaccgc
     7081 gcgtcgtcag ctttcatttc ggtctgcccg cagcggacct catcgcgcgc gtcaagagct
     7141 ggggctcgaa agtcgtcgcc tcagccacga ccgtcgccga agcgcgctgg ctcgaggcga
     7201 acggcgccga cgcgatcgtc gcacagggcg cggaggccgg cggtcaccgc ggcatgttcc
     7261 tgacgaccga tgtcggcacg caaatcggca cgttcgcgct cgtgccacag atcgcgaagg
     7321 ccgtgcgcgt gcccgtcata gccgcgggcg gcatcgcgga tgctcgcggt gtcgcggccg
     7381 cgatggcgct cggtgccgcg ggtgtgcagg tcggcaccgc ttacctgcta tgccacgagg
     7441 ctacgacgag cgccgtgcac cgcgccgcct tgcagagcga tgccgctcgc gagactgcgt
     7501 tgaccaacgt gttcacgggg cggcccgcgc gcggcatcgt caatcgcttg atgcgcgagc
     7561 tcggtccgat cagcgcgctc gcgccggcgt tcccgctcgc cgccgcggcg atcggcgcgc
     7621 tgcgagcgcg cgcggagccg cgcgggtgtg gagacttctc gccgctatgg gccggacaaa
     7681 atgtcggcgg ctgcaaagaa atagcagctg cagagctcac gcgcgagctt gcggaaggcc
     7741 tatagcggcc tatttttcgg cgatgtcgag gtgccccgcg cgctcgtgct tggcgcgcag
     7801 gtaacgctcg ttgtgcgtgt tgctcgtcgt gacgagcggt aggcggccga cgacctcgat
     7861 gccgtggtcg cgcagcgaat tgatcttctg cgggttgttc gtcagcagcc gaatccgcgt
     7921 gtagccgagc tcggtcaaca gcgcggctgc aacgtcgtag ctgcgctcgt cggcgaggaa
     7981 ccccaagtgc tggtcggcgt ctacggtgtc gaggccgccg tcctgtagca cgtacgcgcg
     8041 caatttgttg ggcagaccga tgccgcggcc ttcctggtcg agatagagga gcacgccgcc
     8101 gccgagcgcg gcgatgcgcg cgacagccgt tcgcagctgc tcgccgcagt cgcaacgcaa
     8161 gctgccgagc agatcgcccg tcaggcacgc cgagtgcaag cgcaccggca cggcgctcgc
     8221 cgcgtcgggt tggccgatga cgaccgcgac gtgctcggcg aggctgtgct cgtcgcggaa
     8281 tacggcgatt tcgcactcga cggcgtccgc cagcggcacg cgcgcgcggc tcaagagctg
     8341 tagcgcgtga tggccgaagc gcggcgcatg atgcgcgaca tcgcttaccg caatcgtgag
     8401 tacgccggcc acggcgtcgc cgtcggtgtc gaagccgatc agcgccggta cgagccgccc
     8461 ggccttcgcg agctggaacg cagcggccgc gagcggcgcg ttgcgcggcg ccgggtcgac
     8521 gacgaatgcc tcgtcgtcga cgggcgcgat gccgtcgacg cccgcgagcg tgcgcaggca
     8581 ctcgagatcc gtttcgcgcg gaaacgcaac agccacggcg ccgctgagtc gtcgcgacaa
     8641 cgccgctgca tgcgcgcgct ccgcggtgac gagcatgacg gcgcggccgt cagccgcggc
     8701 gacgagccgc ccgaacaacg gcggctgcag agtctcgatc gcggcgacga gcagaccgcg
     8761 cgcaccgtcg gtgacttgca cggcacgccc gcgccgcaac tcgtcgacgg cgcggtcgaa
     8821 cgtcagtgca gcggggttgc cgttgatttc ctgggtggtt ggcgaggaca tcgcaaaaaa
     8881 cgtgtatttc tagggtgtta ggataccagt tccgacctgt tcggtccgtt tgctgcacat
     8941 ttcaggccac ttccgatgaa ggctaagacc aacgaatccg cggacttagc cgccctcgag
     9001 gccgttgcct ggcagtcgtt gttgtccgcg gcgaaagccg ctcggcaggg ccgcgagctc
     9061 gacgacgaag ccggtgggct gctcgtacgc agcgcccggg agttctacgc gtcgctggat
     9121 tcgccggcga cgattcggcc gttgctcgag ctctatgcgc cgctgtgcgc ggctgggccg
     9181 ctaacgttcg cgcacctcgg ccaaagcctc gacggccaaa ttgcgacggc gtcgggcgat
     9241 tcttactacg tcaccggtcc cgacaacgtg cggcacttgc atcgtctgcg tgcgcttgcc
     9301 gacgcgatcg tcgtcggagc gggcaccgtc gcgcgtgacg atccgcagct gacggtgcgt
     9361 cacgtcgcag gagcgaaccc cgttcgtgtc gtgctcgacc cgtcggcgcg cctcgacccc
     9421 gaccggcgcg tgttccgcga tggcgccgcg ccgacgctcg tcgtgcacgc cgaaggtgtc
     9481 ggctcgcggg caccgggcaa cgcggaggtg ctgcgcgtgc cgcacgcagg cggacgactc
     9541 gcgctcgacg cgctgctcgc gcgcttgcac gagcgagggt tgtcgtcggt gttcgtcgaa
     9601 ggcggcggcg agaccgtgtc gcggttcgtc gagtcgggcc tcatcgatcg tctgcacgtc
     9661 gcgattgcgc cgttgctaac ggggcgcggc cggcccggac tcacgctcgc ggcgcgcgac
     9721 aagatcgccg attgcgtgcg cccggctcat cgcgtgttcg cgatgggcgg cgacgtcttg
     9781 ttcgactgcg atctgcgcgc cgccgcgccg gcggtcagcg agccgagcgg ctcgctgacg
     9841 cgcgtgagct gacgcgctgc gtcaacggcc gatcagcgtc tcttcggtca cgccgagagc
     9901 gcgcaagcgt tcgatcagcg cagccgcctc gtcgcgccgt gcgccgccga gagggcggcc
     9961 gttgctcacg acctgctgcg gtcgcaaaag agtcttcaac gccaggtcgt actgatggcg
    10021 cgcgaaatac agcttcgcga gcgggatata cgggcccgtc gtcgggtacg gcgagcgcat
    10081 cgcaacggcg actcccgtct cgtaggcatc gatcgctcgc gcgtagttac cttcgacggt
    10141 gtagaggtag ccgtagaagt tccagaggtc gagtcggtcc gcgccgtaca ccgcctgcat
    10201 ctcgtcgagt tgcagctcta ctccgcgcag gttgtcggcc gcgagtctgt cgagcgcgac
    10261 ctcgaggccg gccaagtacg cgagttcatt gcgcataacc gcttccagct cctcctgctg
    10321 gcgcggcgtc ggttctgccg ctggattctc ggcgccctcc atggtgaacc ggatgatcgt
    10381 ctggacaccg tcgcggcgca cgcgtttgcc ggccacgaga cggggcaggt aacgccactt
    10441 tgatagcgcg tcgattgccg actgctcgaa ctgcgcatcg tcggatttga ctacgctgac
    10501 gttctcgacg gcgcctgccg tcgtgatgtc gaacttgagc tgcacgtacc ccccggcctt
    10561 ttgctcgaga gccgcgggtg ggtaatgcgg gttgtgccga accagcggta tgacgtccgc
    10621 atcggacgtt tcgatgagct cgacggacgt cggcaggtcg ggcgtcgccg ccgtcgttgg
    10681 cgtggcagcg gccgcagccg tagtgtcgat gaccgctgcg tcgacctgcg ccgagggctc
    10741 gccacccgtc gtgggggaaa gttcttcggg tggcgagcgc gtcagcgaga tgatcgtgac
    10801 gcttgcga
//
LOCUS       DATLEE010000129         3093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9077356, whole genome shotgun sequence.
ACCESSION   DATLEE010000129 DATLEE010000000
VERSION     DATLEE010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9077356"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VM692_02805"
     CDS             complement(<1..200)
                     /locus_tag="VM692_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51122.1"
                     /translation="MRTSVVLSAGLLAALGAAAQCAAATLEVSVVDDEGKPIVDVAVY
                     ATAHSAELGATGVAAEASGPAPT"
     gene            309..2678
                     /locus_tag="VM692_02810"
     CDS             309..2678
                     /locus_tag="VM692_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ51123.1"
                     /translation="MKQYGFRHRILLLAVALVVATQLIVLFPVLDLTKRDSAAQADRT
                     VGLAGVLFDEYMHNRAEGLLTTVTVLVSDYGFKQAVASRGDEATIRSVLRNHANRVRA
                     SIAALLDPNGTVIVSSTADERQVAGFPSVPLATLEDGTRYRVINIGGVPYQTVTAPVK
                     APVTIAWVMLGFPIDDELATHLQSLSELDVSFVSVAGLSTRVLSSTLPESVRAKAVAG
                     LDPTRTDAQRTGVGEDARVSLLRPFAENSDDVYVAMQLSEAMATASYKRVRNFLYAIT
                     GISLLLAITGSFWLAKTVTRPVQDLAEAARRMREGVYNEPINIRTADEFGELAGSFNA
                     MQEAIADRERRIFHQAHHDRLSGLPNRELAVGLLREAIERHKSMAVVSLELDRFGGIV
                     SSLGHRAGDEVIKLAAAALRARLGENEVLGHLSGHEFVIGLPGRDAREAVEWIEFQAD
                     ALRAGVRAGNANISLQVTGGVACYPEHSQDAAELCRRASAARSEALARHETAAVYRLG
                     QEDRALQQIRIVGDFPRALRDNELKLYFQPKLDVATGDIYGAEALVRWQHPELWLLFP
                     DSFIAAVEQAGSIAHLTRWVLREAVARCAAWRNQGVMLGVAVNISVDDLTDEYLPYYL
                     LEITQKHQLAPHTLTLEVTESAIMHNVQKSLAVVNVIHELGFRLAIDDFGTGHSALSQ
                     LRRLPVDELKIDKSFVMKSGDAKDDAILRATIDLAHQLGLQVVAEGVENDAAIARLAA
                     LGCEHLQGYGIGKPIPHEQFLQWLSQRRSAGRPSVVALPVPIVDVKARS"
     gene            complement(2675..>3093)
                     /locus_tag="VM692_02815"
     CDS             complement(2675..>3093)
                     /locus_tag="VM692_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HUQ51124.1"
                     /translation="LDIKPYVAYTDAIGDANSGWLAKGDAPRDPIPAFQVHWDAAAVQ
                     RAEWVEARTGLNLRERATATLALGPEPHPYRRIRREQSGFTLCVKDWRLRFSVEQRDV
                     RVASVESGVRKSLLVRGGDAADAELALHREFYSLFG"
BASE COUNT          510 a         1086 c         1013 g          484 t
ORIGIN      
        1 gtgggcgcgg gccccgacgc ctcggccgcg acgcccgtcg cgcccagctc cgcggagtgc
       61 gccgtcgcgt agacggcgac gtcgacgatg ggcttgcctt cgtcgtcgac tactgaaacc
      121 tcgagcgtcg ccgcggcgca ctgggccgca gcgccgaggg cggccagcag accggcggac
      181 aacacaaccg acgtgcgcat cgcgaagttc gtattactaa ggagtccgga tgttatgtcg
      241 acgctttggg cgcatccgtg accgagcgca cacaaccctt gctagggcct ccgtagtatc
      301 gcaactgcat gaaacagtac ggctttcgtc acagaattct gctgctcgcc gtcgcgctcg
      361 tcgtcgcgac gcagctcatc gtgctgttcc ccgtgctcga cctcaccaag cgcgattccg
      421 ccgcgcaggc ggaccggacc gtcggcctcg ccggcgtgct gttcgacgag tacatgcaca
      481 atcgagccga aggattgctg acgaccgtca ccgtcctcgt ctccgactac gggttcaagc
      541 aagccgtcgc gagccgcggc gacgaagcca cgattcgctc cgtgctgcgt aatcacgcga
      601 accgcgtgcg cgcgagcatc gcggctttgc tcgatccgaa cggcaccgtc atcgtcagct
      661 cgacggccga cgagcgtcag gtggcgggat tcccgagcgt cccgctcgcg acactcgagg
      721 acggcacgcg ctatcgcgtc atcaacatcg gcggcgtgcc gtatcagacc gtgacggcgc
      781 ccgtgaaagc gcccgtgacg atcgcttggg tcatgctcgg cttcccgatc gacgacgagc
      841 tcgcgacgca tttgcagagc ctgtcggagc tcgacgtgtc attcgtctcg gtcgccggtc
      901 tgtcgacgcg cgtgctgagc tcgacgctgc ccgagagcgt gcgagcgaag gccgtcgccg
      961 gcctcgatcc gacacgcacc gacgcgcagc gcaccggcgt cggtgaagac gcgcgcgtct
     1021 cgctgctgcg gccgttcgcc gagaacagcg acgacgtcta tgtggcgatg cagctctccg
     1081 aggcgatggc gacggcgtcg tacaagcgcg tccgcaattt cctctacgcg atcacgggca
     1141 tctcgctgct gctcgcgatc acgggctctt tctggctggc caaaacggtc acgcgccccg
     1201 tgcaagatct cgccgaagcg gcgcggcgca tgcgcgaagg cgtctacaac gagccgatca
     1261 acatccgcac ggccgacgag ttcggcgagc tggccggcag cttcaacgcg atgcaagagg
     1321 cgatcgccga tcgcgagcgg cgcatctttc accaggcgca tcacgacagg ctgtcgggcc
     1381 taccgaaccg cgagctcgcc gtggggctgc tgcgcgaagc catcgagcgg cacaagtcga
     1441 tggccgtcgt aagtctcgag ctcgaccgct tcggcggcat cgtgtcgtcg ctcggccacc
     1501 gcgccggcga cgaggtcatc aagctcgcgg ccgccgcttt gcgcgcgcgg ctcggcgaga
     1561 acgaggtgct gggacatctc agcggtcacg agttcgtgat cggcctgccg ggacgcgacg
     1621 cgcgcgaggc cgtcgagtgg atcgagtttc aagccgacgc gctgcgcgcc ggcgtgcgcg
     1681 ccggcaacgc caacatctcg ctgcaggtca cgggcggcgt cgcttgttac cccgagcaca
     1741 gccaggacgc cgccgagctc tgtcgccgcg cgtcggcggc gcgatcggaa gcactggcgc
     1801 gccacgagac cgcggccgtg tatcgtttgg gtcaagaaga ccgcgcgctg cagcagatcc
     1861 gcatcgtcgg cgacttccct cgcgcgctgc gcgacaacga gctcaagctg tacttccaac
     1921 cgaaactcga cgtcgcgacg ggcgacatct acggcgccga ggcgctcgtt cgctggcagc
     1981 accccgagct ctggctgctg ttccccgatt cgttcatcgc ggccgtcgag caggccggca
     2041 gcatcgcgca tctgacgcgc tgggtgttgc gcgaagctgt cgcgcgctgc gcggcgtggc
     2101 gcaatcaggg cgtgatgctc ggcgtcgccg tcaacatctc ggtcgacgac ctgaccgacg
     2161 agtacctgcc ctactacctg ctcgagatca cgcagaagca ccagctggcc ccgcacacgt
     2221 tgacgctcga ggtcaccgag agcgcgatca tgcacaacgt ccagaagtcg ctggccgtcg
     2281 tcaacgtcat ccacgagctc ggtttccgct tggcgatcga cgacttcggc acgggccatt
     2341 cggcgctgtc gcagctgcgg cgcctgcccg tcgacgagct caagatcgac aagtcgttcg
     2401 tcatgaagag cggcgacgcg aaggacgacg cgattttgcg cgcgacgatc gacctcgcgc
     2461 accagctcgg gctccaggtc gtcgccgaag gcgtcgagaa cgatgccgcg atcgcgcggc
     2521 tcgcggcact cggctgcgag cacctgcagg gctacggcat cggcaagccg attccgcacg
     2581 agcagttctt gcaatggctc tcgcagcgcc gcagcgcagg ccggcccagc gtcgtggcgc
     2641 tgccggtgcc gatcgtcgac gtcaaagcgc gcagctagcc gaacagcgaa tagaactcgc
     2701 ggtgcaacgc gagctccgcg tcggcagcgt cgccgccgcg cacgagcagc gacttgcgaa
     2761 cccccgactc gaccgacgcg acgcgcacgt cacgctgctc gacgctgaac cgcaagcgcc
     2821 aatccttgac gcacaacgtg aagccgctct gctcgcggcg aatccgccga tacgggtgcg
     2881 gctcggggcc caaggccagc gtcgccgtcg cgcgctcgcg caagttgagc cccgtgcgcg
     2941 cctcgaccca ctcggctcgt tgcacggctg ccgcgtccca gtgcacttgg aacgccggaa
     3001 tcggatcgcg cggcgcgtcg cccttcgcga gccagccgct gttggcgtcg ccgatcgcat
     3061 ccgtgtaggc aacatacggc ttgatgtcga gca
//
LOCUS       DATLEE010000130         5794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8268053, whole genome shotgun sequence.
ACCESSION   DATLEE010000130 DATLEE010000000
VERSION     DATLEE010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8268053"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VM692_02820"
     CDS             complement(<1..674)
                     /locus_tag="VM692_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUQ51125.1"
                     /translation="MTANGSEGLVARATALVPLLRDKAQANETGRRLSPEVFDALAAA
                     DVFRMTAPKRFGGFEADFVTQCEVLAEVARGCGSSSWVATIFSAMSWLVGTLRDEAQE
                     EIFDSKDPRISGVFSPTGNAVRAKGGFIVNGRWGFNTGGHGSNWTVVNAIAPNAAGVE
                     LPTCMLVPSKELQRLDDWHASGMAATGSSTIVAKDLFVPEHRTCELPSMIEAHYPQRH
                     NSTNLY"
     gene            complement(700..1848)
                     /locus_tag="VM692_02825"
     CDS             complement(700..1848)
                     /locus_tag="VM692_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017974953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HUQ51126.1"
                     /translation="MRRSASKILTTHVGALPGPLETWEGEASDAALRAAVQGVVAAQQ
                     EAGTDVVNEGELTKGGNWVTFINSRLSGFVGVPAEGTLRLLSASKDWQQFDDFYAKAM
                     AGGTLFEQTASAPVQTSRIRDWICREPIRYTGQASLQREIDLLKASLGSHAVGDAFLT
                     STAPASIEVGRKNEYYKSDEEFVYALADALRVEYETIAKAGLNVQIDDAWLPALWDRI
                     GVQMGLEAYQRYCMLRVEALNHALENVPEEQIRYHLCWGSWHGPHAHDIPMADMVDIM
                     LSVKAQMYLFEAANARHEHEYTLWERVPLPKGKILAPGVVSHATALIEHPELVSQRIQ
                     RFAKLVGRDNVVASTDCGLGLRCHPQIAWAKLAALSQGAALASRALGY"
     gene            complement(1855..3000)
                     /locus_tag="VM692_02830"
     CDS             complement(1855..3000)
                     /locus_tag="VM692_02830"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HUQ51127.1"
                     /translation="MKHDSSDEIRGTVERRDLLKLAGGVALASAAQWPSLGAAAPSRA
                     YVPGSSTHEYDKMFVAVVTPYKPGTEDIDEAAYRSYMRYWAQPKFIDAGGAVLVSPEA
                     GEAFYLTQSEKLKLAEIALEELGDKTIVFSGVMQTTTRACIEEAKALKRAGVHGLFLM
                     PPIGTIDIVAAWDSKKNPEVWIDQLREIADAVNLPVIIHPTGGKFFALPLESGMKAVD
                     AVPQIVGYKMITEDFDDMARALRAYTKRHVAILPAGANNMPPAIQNGWFDGTVSGSWN
                     YAMEKTVDAIVAGRDEKNAAKADGLWADGLMALHRTVTNSDKGYRLHSAYKLCTWMRG
                     LIPNPFLRAPQRRLPVAEVVELRDALKAAHYEVISDTVMRKAYPQLS"
     gene            3288..4226
                     /locus_tag="VM692_02835"
     CDS             3288..4226
                     /locus_tag="VM692_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HUQ51128.1"
                     /translation="MACLLDPLTAQTAREAEHPTIEEITVIGRFPGPPLWKVSSGEHA
                     LWIFGDLTPVPKGLTWDPRNAQRIVERADAVIGPMRIRAPTYNPIKIFRALRAARRLA
                     RNPEGSTLKDVLPADLYARYDALRQKHMPDDKRGEDLRPALAVLRLHGAALDDVGLTL
                     DTGVMKTVERLMKRSDAEEVQAKVETEAATVLRELEKVTREAELNCFATTLTTLETDL
                     DGMKERAQAWAIGDVDALERFDYPDAQGDCLEMLFSSSGLKDLGDELFAKWLGEAEHA
                     LATYDTTFTVLPMRELVAADGLLAQLAAKGYTVTTP"
     gene            complement(4238..>5794)
                     /locus_tag="VM692_02840"
     CDS             complement(4238..>5794)
                     /locus_tag="VM692_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51129.1"
                     /translation="VETALAELPAAEPAYEKCWWPAHDARNRRWIAAAQPVLAAHEEA
                     LTARLTELYGEDLARPFPVDVVSYGSFSGADSVVDPNHLLVSSVVPANTGNAALEILF
                     HEASHTVFGPGLDGRLWTELEAAAKADGAPLPRDFWHAMLFYTTGRAVQAQLAERGID
                     YQPYVYAQGLFERSWPWFREPLERWWQPYLGGSAPRMESLRQLVAAHKLPDRGGAFVA
                     ASRTFVFYSDLVTNLHDFLVWNARSPEPIEPASDCLAQLPDAQRAAFTHARDYYAGAF
                     ANGAGELVLLSMRWRLAKLGDVSLTDGAQIDAAIAELAPATPAYEACWWREHDARNRR
                     WIASVLPLLEANESPLRVRLAELYGQELARSLPVDVVGYAGIGEAATVVNPPQLLISS
                     AAPANAGNGALEVVLHEASHTLFGPRTPGPLWTALQQASSAAAKPLADELTHLLLSVT
                     TGKAVQARFIERGIRDYEPYVYREGLFGRAPPALRDVLERVWLPSVDARVPTAEAVRQ
                     LVEALPAPVR"
BASE COUNT          989 a         1948 c         1881 g          976 t
ORIGIN      
        1 taatacaagt tcgtcgagtt gtgacgctgc ggatagtgcg cttcgatcat cgacggcaac
       61 tcgcacgtgc ggtgctcggg gacgaacaaa tccttggcga cgatcgtgct gctgcccgtg
      121 gcggccatgc cgcttgcgtg ccaatcgtcg aggcgctgca gctccttgct gggcacgagc
      181 atgcaggtcg gcagctcgac gccggccgcg ttcggcgcga tggcgttgac gacggtccag
      241 ttcgagccgt ggccgcccgt gttgaaaccc cagcggccgt tgacgatgaa gccgcccttc
      301 gcgcgtacgg cgttgcccgt cggcgagaag acgccgctga tacgcgggtc cttcgagtcg
      361 aaaatctctt cctgcgcttc gtcgcgcagc gtgccgacga gccacgacat cgcgctgaag
      421 atcgttgcga cccacgacga cgagccgcag ccgcgcgcca cttcggccag cacctcacat
      481 tgcgtgacga agtcggcttc gaacccgccg aaacgcttcg gcgccgtcat gcggaacacg
      541 tcggccgcag ccagtgcgtc gaaaacctcc ggtgacaggc gccgcccggt ttcgttggct
      601 tgcgctttgt cgcgcagcag cggcacaagc gcggtcgcgc gcgcgacgag cccttccgag
      661 ccgttggcag tcataagcga agctccggat cgaggtgagt cagtagccta gcgcacggct
      721 cgccagagcg gcaccctgcg acaaggccgc gagcttcgcc caggcgatct gcggatgaca
      781 gcgcaggcca agaccgcagt ccgtcgaggc gacgacgttg tcgcggccga cgagcttcgc
      841 gaagcgctgg atgcgctgcg atacgagctc gggatgctcg atcagtgccg ttgcgtgcga
      901 gacgacaccg ggggcgagga tcttgccttt cggcagcggc acgcgctccc agagtgtgta
      961 ctcgtgctcg tggcgcgcat tcgcagcctc gaacagatac atctgcgcct tgacgctcag
     1021 catgatgtcg accatgtcgg ccatcgggat gtcgtgcgcg tgcggcccgt gccagctgcc
     1081 ccagcagagg tgatagcgga tctgctcctc gggcacgttc tcgagcgcgt gattcagcgc
     1141 ctcgacgcgc agcatgcaat agcgctgata ggcctcgagc cccatctgca cgccaatgcg
     1201 gtcccagagc gccggcagcc acgcgtcgtc gatctgcacg ttgaggccgg ccttcgcgat
     1261 cgtctcgtac tcgacgcgca gcgcgtcggc cagcgcgtag acgaactcct cgtcgctctt
     1321 gtagtactcg ttcttgcgcc cgacctcgat gctggccggc gccgtcgacg tcaagaacgc
     1381 gtcgccgacg gcgtgcgagc ccagcgacgc tttgagcagg tcgatctcgc gctgcagtga
     1441 agcttgaccg gtgtagcgaa tcggctcgcg gcagatccag tcgcggatgc gtgaagtctg
     1501 caccggcgcg ctcgcggtct gctcgaacag cgtgccgccg gccatcgcct tcgcgtagaa
     1561 gtcgtcgaac tgctgccaat ccttgctcgc gctcagcaac ctgagcgtgc cttcggccgg
     1621 cacgccgacg aaccccgaca gacgcgagtt gatgaaggtg acccagttgc cgcctttcgt
     1681 cagctcgcct tcgttgacga cgtcggtgcc ggcctcttgc tgcgcggcga cgacgccctg
     1741 cacggccgcg cgcagcgccg cgtcgctggc ctcgccctcc caggtctcga gcggccccgg
     1801 cagggcgccg acgtgcgtcg tcaggatctt cgacgcgctg cggcgcatag gccgctagga
     1861 caactgcggg tacgccttac gcatgacggt gtcggagatg acctcgtagt gcgcggcttt
     1921 cagcgcgtcg cgcaactcga cgacttccgc gaccggcaac cggcgctgcg gtgcgcgcag
     1981 gaacggattc ggaattaggc cgcgcatcca agtgcatagc ttgtaagcgc tgtggaggcg
     2041 atagcccttg tccgagttcg tcacggtgcg atgcaacgcc atcaagccgt cggcccacaa
     2101 gccgtcggcc ttcgccgcgt tcttctcgtc acggccggcg acgatcgcgt cgacggtctt
     2161 ctccatcgcg tagttccacg agccgctgac ggtgccgtcg aaccagccgt tttggatcgc
     2221 gggcggcatg ttgttcgcgc cggcgggcag gatcgcgacg tggcgcttcg tgtaagcgcg
     2281 caatgcgcgc gccatgtcgt cgaagtcttc ggtgatcatc ttgtacccga cgatctgcgg
     2341 gaccgcgtcg accgccttca tgccggattc gagcggcagc gcgaagaact tgccgccggt
     2401 tgggtggatg atcacgggca gattcaccgc gtcggcgatc tcgcgcagct gatcgatcca
     2461 cacctcgggg ttcttcttgg aatcccaagc ggcgacgatg tcgatcgtgc cgatcggcgg
     2521 catcaagaac aggccgtgca cgccggcacg cttgagcgcc ttcgcttcct cgatgcaggc
     2581 cctggtcgtc gtctgcatga ccccgctaaa cacgatcgtc ttgtcgccga gctcctcgag
     2641 cgcaatctcc gcgagcttca gcttctcgct ctgcgtcagg tagaacgcct cgccggcttc
     2701 cggcgagacc agcacggcgc cgcccgcatc gataaatttc ggctgcgccc agtagcgcat
     2761 gtaggaacga tacgccgctt cgtcgatgtc ctcggtgccg ggcttatacg gcgtgacaac
     2821 ggcgacgaac atcttgtcgt actcgtgcgt gctggagccg ggaacgtacg cgcgcgaagg
     2881 agccgcggct ccgagcgagg gccactgcgc cgcactcgcc agcgcgacgc cgcccgcgag
     2941 cttcagcagg tctcggcgct caacggttcc gcggatttcg tcgctcgaat cgtgcttcat
     3001 gatccccctc actccgccgc aacggcagcg aacaacaata agacacggct cgatgctacg
     3061 ccccaagtac tctcagtgcg aggcagatcg cggcgagccg cgcacgcagg agaaataagt
     3121 atcattagtc tagtattatc catatgttac aaatcattat taaaccaaaa aaaatcgtcg
     3181 gcgcgcaccg atcgagcgac gccgcttgct aacatcgacg catgcggcca caccatcgct
     3241 cgagctcagc cacggcaagt cgaaggcggc tctacgtcgt tgcgatcgtg gcatgccttc
     3301 tcgatcccct gacggcgcag acggcgcgag aagccgagca ccccacgatc gaggagatca
     3361 cggtcatcgg gcggtttccg gggccgccgc tgtggaaggt ttcatcgggc gagcatgccc
     3421 tgtggatctt cggcgatctg acgccggtgc cgaaaggttt gacctgggat ccacgcaacg
     3481 cgcagcgaat cgtcgagcga gccgatgccg tcatcggccc gatgcgtatc cgagcgccga
     3541 cgtacaaccc gatcaagatc tttcgtgcgc tgcgggcggc gcggcgcctc gcgcgcaatc
     3601 ccgaaggctc gacgctgaag gacgtgctgc ctgcggatct ctatgcgcgt tacgacgcgc
     3661 tgcggcagaa gcacatgccc gatgacaagc gcggcgagga cttgcgccct gctcttgccg
     3721 tgctgcgcct gcacggcgcc gcgctcgacg acgtcgggct cacgctcgac accggcgtca
     3781 tgaaaaccgt cgagcgcctc atgaagcgct cggacgccga ggaagtgcaa gcgaaagtcg
     3841 agaccgaagc cgcgaccgtt ctcagagagc tcgagaaggt gacgcgcgag gcagagctga
     3901 attgctttgc tacgacgctg acgaccctgg aaacggatct cgacggcatg aaggaacgcg
     3961 cacaagcgtg ggcgatcggc gacgtcgacg cgctcgagcg cttcgactat cccgacgcgc
     4021 agggcgactg cctcgagatg ttgttctcgt cgtcgggcct caaggacctg ggcgacgagc
     4081 tgttcgcgaa gtggctcggt gaagccgagc acgcgctcgc gacttacgac acgacgttca
     4141 cggtgctacc gatgcgcgag ctcgtcgccg ccgacgggct gctcgcgcag ctcgcggcga
     4201 aaggttacac cgtcactacg ccgtagccgc gccgagacta gcgcaccgga gccggcaatg
     4261 cttcgacgag ctgccgcact gcttccgctg tcggcacgcg cgcgtcgacc gacggcaacc
     4321 acacgcgctc gagcacgtcg cgtagcgccg gcggggcgcg cccgaacagc ccctcccgat
     4381 acacgtaagg ctcgtagtct cgtatgcctc gctcgatgaa ccgggcttgc actgccttac
     4441 ccgtcgtcac ggagagcaac aggtgcgtga gctcgtccgc cagcggcttg gccgccgcgc
     4501 tagaggcttg ctgcaacgcc gtccagagcg gtcccggtgt tctcggcccg aacagcgtgt
     4561 gcgacgcttc atgaaggact acttcgagcg ccccgttgcc cgcgttagcg ggagcggcgc
     4621 tcgaaatcag aagctgcggc gggttgacga cggtggcggc ttcgccgatg ccggcgtagc
     4681 cgacgacgtc caccggcagc gagcgcgcaa gctcttggcc gtagagctcg gcgagccgca
     4741 cgcgcaaagg actctcgttg gcttcgagca gcggcagcac gcttgcgatc caacgccggt
     4801 tgcgcgcgtc gtgctcgcgc caccagcagg cttcgtaggc cggagtcgcc ggagcgagct
     4861 cggcaatcgc ggcgtcgatc tgcgcaccat cggtaaggct cacatcaccg agctttgcga
     4921 gccgccaacg catcgagagc agcacgagct caccggcgcc gttcgcgaat gcgcccgcgt
     4981 agtagtcgcg cgcatgcgtg aacgccgcgc gctgcgcgtc gggtaattgg gcaaggcaat
     5041 ccgacgcggg ctcgatcggc tccggcgagc gcgcgttcca gaccaggaag tcgtgcaaat
     5101 tcgtgacgag atcgctgtag aacacaaagg tgcggctcgc cgcaacgaag gcgccgccgc
     5161 ggtccggcag cttgtgtgcc gcaacgagtt gccgcagcga ttccatcctg ggtgcgctgc
     5221 cgccgaggta cggttgccac catcgctcga gcggctcgcg aaaccacggc cacgagcgct
     5281 cgaacaagcc ttgcgcgtag acgtacggct ggtagtcgat cccgcgctcg gccaattgcg
     5341 cctgcaccgc acgaccggtc gtgtagaaca gcatcgcgtg ccagaaatcg cgcggcagcg
     5401 gagcaccgtc ggctttcgcg gctgcctcga gctccgtcca cagccgcccg tcgagcccgg
     5461 gcccgaacac cgtgtgcgag gcttcgtgga acagaatttc gagcgccgca ttgccggtgt
     5521 tcgccggcac cacgctggac acgagcaggt gattcggatc gacgacactg tcggctccgc
     5581 tgaagctgcc gtagctcacg acgtcgacgg gaaatggccg cgcgagatct tcgccgtaga
     5641 gctccgtcaa gcgcgcggtc agagcttcct cgtgcgccgc gagcaccggc tgcgcagccg
     5701 ctatccagcg ccggttgcga gcgtcgtgcg ccggccacca gcacttctcg tacgccggct
     5761 cagcggccgg cagctccgcg agcgcggtct cgac
//
LOCUS       DATLEE010000131         2316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_143267, whole genome shotgun sequence.
ACCESSION   DATLEE010000131 DATLEE010000000
VERSION     DATLEE010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143267"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VM692_02845"
     CDS             <1..850
                     /locus_tag="VM692_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HUQ51130.1"
                     /translation="LGDMGFDFARGRLDRSTHPFTMMAGDHDVRLTIRSSADDPLRAI
                     FATLHEGGHALYDQGLPRELHGTLLADAPSMGVHESQARLWENHVGRSAALWRRYFPR
                     LQTAFADVLSSLDARSFHRAINVVVPGVNRVAADEATYNLHVLVRYELEVALLAGDLA
                     ATDLPGSWDELYRKYLGVSAKSPRDGCLQDVHWALGEFGYFPTYTIGNLYAAQLADAY
                     DVDHDLDAELAGGNLTALRDWLAANIYVHGAELPAEALIERITGRSLDVEPFFSRLER
                     RVAELD"
     gene            964..1773
                     /locus_tag="VM692_02850"
     CDS             964..1773
                     /locus_tag="VM692_02850"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HUQ51131.1"
                     /translation="MRLVIRNVALSDDEIEAFFEIGWIVRRALFRADEVARMRACFDE
                     LERMAGELPETALHQGSYFVLANKNGMRVINRVVWAGGSQRYLLDVGSDERLTVPCAQ
                     LLASDAMDHLLSQAHFKRPRDGVAFGWHQDIQHRDKGNGTWTDVNGRGSFVQTVIALD
                     EMTQDSGPLQFMPGSTKWGRVEFGDHAAHGESGTGKPAQFREQDAVTVTARPGDALFF
                     GPYTAHASFENTSERYRRILINGYAFPGANRRVYPGDGAGRRLAVAPSRVR"
     gene            complement(1781..>2316)
                     /locus_tag="VM692_02855"
     CDS             complement(1781..>2316)
                     /locus_tag="VM692_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HUQ51132.1"
                     /translation="HEFFLAVAFPHDEMRILDYNRVVRDLNGLSPDALLARIRESFDV
                     APLPTAKSPVQPETFAMFVAGHWHELKIRAALVPRHDPVASLDVSLLQDHLLAPILSI
                     GDPRTDARIDFVGGVRGLTELERRVQSGRAAAAFALHPTRMEQLMAVADAGKLMPPKS
                     TWFEPKLADGLLSHVLD"
BASE COUNT          388 a          768 c          786 g          374 t
ORIGIN      
        1 gctcggcgac atgggcttcg atttcgcgcg cgggcggctc gatcgctcga cgcacccgtt
       61 cacgatgatg gccggcgacc acgacgtgcg tctgacgatt cgctcgagcg ccgacgatcc
      121 gctgcgcgcg atcttcgcga cgctgcacga gggcgggcac gcgctctacg atcaaggcct
      181 gccgcgcgag ctgcacggta cgttgctcgc cgatgcaccg agcatgggcg tgcacgaatc
      241 gcaggcgcgg ctctgggaaa atcacgtcgg gcgcagcgcc gctctgtggc ggcgctattt
      301 cccgcggctg cagacggcgt tcgccgacgt cttgtcgtcg ctcgacgcgc ggagcttcca
      361 tcgagccatc aatgtcgtcg tgcccggcgt caatcgcgtc gccgccgacg aggctacgta
      421 caacttgcac gtgctcgtcc gctacgagct cgaggtcgcg ctgcttgcag gtgatctcgc
      481 ggcaacggat ctccccggct cctgggacga gctctaccgc aaatacctcg gggtcagcgc
      541 gaagtcgccg cgcgacggct gcttgcaaga cgtccactgg gcgctcggcg aattcggcta
      601 cttcccgacc tacacgatcg gcaatcttta cgccgcgcag ctcgccgacg cgtacgacgt
      661 cgatcacgac ctcgatgccg agctcgccgg cggcaacttg acggcgctgc gcgactggct
      721 ggccgcgaac atctatgtgc acggtgccga gctgccagcc gaagcgctga tcgagcgcat
      781 cacgggccgc tcgctcgacg tcgagccgtt cttcagccgc ctcgagcgcc gcgtcgccga
      841 gctcgactag ctgcgacgga tcggcctgca ttaagtcacg aagcgcggcg cgggccgcga
      901 cgatgcgcta taagtacggc gtcggtcact gcaagaacaa caacaattcg cgatactgac
      961 agcatgcgac tcgtgattcg caacgtcgcc ttgagcgacg acgaaatcga ggccttcttc
     1021 gagatcggct ggatcgtgcg gcgagcgctg ttccgcgccg acgaggtcgc gcgcatgcgc
     1081 gcctgcttcg acgagctcga gcgcatggcc ggcgagttgc cggagaccgc gctgcatcaa
     1141 ggctcgtatt tcgtgctcgc gaacaagaac ggcatgcgcg tgatcaatcg tgtcgtctgg
     1201 gccggcggat cgcagcgcta cctgctcgac gtcggcagcg acgagcgcct gaccgttcct
     1261 tgtgcgcaat tgctcgcgag cgacgcgatg gatcatctgt tgagtcaggc gcatttcaag
     1321 cgtcctcgcg acggcgtcgc gttcggctgg caccaagaca ttcagcatcg cgataagggc
     1381 aacggaacct ggaccgacgt caacggccgc ggcagcttcg tgcagaccgt cattgcgctc
     1441 gacgagatga cacaagacag cgggccgttg caattcatgc cgggcagcac gaagtggggg
     1501 cgagtcgagt tcggcgacca cgcggcgcac ggcgagtccg gtaccggcaa gccggcgcag
     1561 tttcgcgagc aagatgccgt gaccgtcacg gcgcggcccg gcgacgcctt gttcttcggg
     1621 ccgtacacgg cacacgcgag cttcgagaac acgtcggagc gctatcggcg catcctgatc
     1681 aacggctatg cgttcccggg cgcgaatcgc cgcgtttatc ccggcgacgg cgccgggcga
     1741 aggttggccg tggcgccgtc gcgcgtgcga tagcaactcc tcagtcgagc acgtgcgaga
     1801 gcaggccgtc ggcgagcttc ggctcgaacc acgtcgactt cggcggcatc agcttgccgg
     1861 catcggcgac ggccatcagc tgctccatgc gcgtcgggtg cagcgcgaac gcggcggccg
     1921 cgcggcccga ctgcacgcgc cgctcgagct cggtgagccc gcgcacgccg ccgacgaaat
     1981 caatgcgcgc gtcggtgcgc gggtcgccga tgctcaagat cggcgcgagc agatgatctt
     2041 gcagcaggct gacgtcgagg ctcgcgacgg gatcgtggcg cggcacgagc gccgcgcgaa
     2101 tctttagctc gtgccaatgc cccgcgacga acatcgcgaa cgtctcgggt tgcaccggcg
     2161 acttggcagt cggcagcggc gcgacgtcga acgactcgcg gatgcgcgcg agcagcgcgt
     2221 cgggcgacag cccgttcaag tcgcggacga cgcggttgta gtcgaggatg cgcatctcgt
     2281 cgtgcgggaa ggcgaccgcg aggaagaact cgtggc
//
LOCUS       DATLEE010000132         6608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2377852, whole genome shotgun sequence.
ACCESSION   DATLEE010000132 DATLEE010000000
VERSION     DATLEE010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2377852"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5379)
                     /locus_tag="VM692_02860"
     CDS             complement(<1..5379)
                     /locus_tag="VM692_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HUQ51133.1"
                     /translation="MGFERIMEHRGAFAARLRALLRAVLVFGLGTVAAAQQGARAPLT
                     ITNITPGGTNVPAARQIVLQFNRPVVPVGRMDRTAAEIPIVITPPTVCQWRWLDTSAL
                     ACQLADGDELKLATRYTLTVNEGIMAEDGATTAGVRRHEFTTERPRLSYPGFATWRSP
                     GMPVIRAVFTQAVSESSVREHLFMRHRGAARAAVTVEADTQQREVPRFVRLPGERVFV
                     DFGERQTARVDDRPTGAGGETARRVWLVRPSAELPLDTRVELVVEPGLEPAVGTERGD
                     ENRVAVEFQTFPELEFLGVVCRAVDDSELVLPASNGSAGACNPLGGVGLQFSAPVLGS
                     EIKNHIELTPDLAGGRTDYDPWANWRDYSLLEQPHERGRPYTVWLPERLKAAEPYRLR
                     TRDPAEGPKDEFGRSLRAPLDFAFATSHRPPDYTLVHTTAVLEQGVDSEVPLYVTNLE
                     RYSLSYRALTPAGARADLVLSRELPAIADVQFGVPLGVRAVLGAGSGAIFGRLETSPA
                     LARSGWEEPLFAAVTPYQLHVKLGHFNSLVWVTDLTTGEPVRDAAVTVYVDRLRDLSA
                     ALPPKAQAATDATGVATFPGTRELDADLALSGNGYGCNAGRSESCQRLFVRVDGPKGM
                     ALMPLEYRFAVDSYRASRYTVFEQSQREYGHLHAWGTTAQGVYRAGDTIDWKIYVRDQ
                     DNESLVVAPTGPYQLEIVDPTGQVVHTVADATLSKFGALSGRYAIPQSAAVGWYQFRL
                     TPKLAAASANASGDYDADALVRFPLRVLVSDFTPAPFGVRTTLNGDLFEAGQDVGVET
                     RATLFSGGPYVDAEARVTAQLTARPFRTAHPAAAGFQFDSGMQQPAALVLSQQQTQVD
                     AQGEARHTFRLGADLGAQVVHGTISVEGAVRDDRGRYVASAASAEFVAVDRLVGLRST
                     EWVFKEDEPATVQYLVVDGRGAPAAGTAVTIDVERLETKAARVRGAGTAYLTEFIDEW
                     VAAGECGGTSSSAPLNCTFTPEAPGSYRLTATIRDTRDRPHKTVLQTWVLGKGQLVWR
                     SGNDDALEMVPEQSTYEIGDTARYLVKNPYPGARALVTVERYGILKQWVQTFDSSTPV
                     VEFEVEKDFMPGFYLSVLVMSPRVEGPPPEQGELDLGKPSFKLGYLDVPVKDPYKEIR
                     VEAHADRAVYKPGDKVRVRVQARPREREQREPIEIAVAVLDESVLDLIQDGTRYFDPY
                     AGFYTQDGLDVRNFSLLTRLVGRQKIDLKGANPGGDGGAAFSMRSVFKYVSYWNPSIE
                     LDSRGNGSFELEVPDNLTGWRVLVMAATPTDRLGLGEATFRVNLPTEVRPVMPNQVTE
                     GDRFDAAFSVMNRTDAARDIRVTIAAADGVAAPVAHAQTVHLEPYARTTVLAPVQAAR
                     VATTRDVPTGRIEFTVTARDATDGDGLRHALVVNKRRSFEVGATYGTFDDARATESLE
                     FPERIRTDAGDVGVTLSPSVIGNIDGAFRYLRDYPYYCWEQRLTKGVMASHFTHLRDY
                     LPPDLEWPEAAALPEATLEQAASFQAPSGGMTYYVPQDQYVSPYLSAYTALAFDWLRE
                     DGHAIPEAVETKLHEYLDSFLKRDVAPDFYTRGMASTVRAVALAALAKRGKVTLADLQ
                     RYRAHVESMSLFGKAHYLQAALAVAGGETIAREVEQIILQSSVRSGGKLAFNEVLDDG
                     YLRISATPLNSQCAILSSLTKGAMSESQIGMAGDVPFELVRAITQARGSRDHWENTQE
                     NMFCMSSLLDYAQRYESVTPAMRVTAAVGDAPLGRAQFDSVRDAAVTLSRPIGPADPG
                     TRTQMTVER"
     gene            complement(5469..>6608)
                     /locus_tag="VM692_02865"
     CDS             complement(5469..>6608)
                     /locus_tag="VM692_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUQ51134.1"
                     /translation="PAAAAPHSAAKPIRFATLAAGGTAPRATIEPHDLVLLPYSSGTT
                     GLPKGVMLTHANLVANILQVDAAGHFNDGEDTTVAFLPFFHIYGLTVIVFMSLWAGAT
                     VVVMPKFELEPYLALVERHRATVLHVVPPVVLAFAKHPAVAGRDFSRVRMLFSGAAPL
                     GADITEQAMRRIDCVLQQGYGMTEASPATHVATADVARRKLGSIGAPVANTDCRVVDI
                     ETGRDVEPGSDGEIWVRGPQVMRGYLNRPDETRATVDADGWLHTGDIGHADADGHFYV
                     VDRLKELIKYKGMQVPPAELEAALLAHPAVADAAVVPLTDAECGEIPRAFVVLKGKAT
                     ADELMAHVAACVTHYKKVRRVDFVDAIPKSPSGKILRRLLRDRPT"
BASE COUNT         1008 a         2304 c         2253 g         1043 t
ORIGIN      
        1 acgctcgacc gtcatttgcg tgcgtgtacc gggatcggcc gggccgatcg gccggctcag
       61 cgtcacggcg gcgtcgcgca cgctgtcgaa ctgcgcgcgg ccgagcggcg cgtctccgac
      121 ggccgccgtc acccgcatcg ccggcgtcac gctctcgtag cgctgcgcgt aatcgagcag
      181 cgagctcatg cagaacatgt tctcctgcgt gttctcccag tgatcccggc tgccgcgcgc
      241 ctgcgtgatc gcgcgcacga gctcgaacgg cacatcgccg gccatgccga tctggctctc
      301 gctcatggcg cccttcgtca acgagctcaa aatcgcgcat tgcgaattca gcggtgtcgc
      361 ggaaatgcgc agatagccgt cgtcgagcac ttcgttgaac gcgagcttgc cgccgctgcg
      421 cacgctcgac tgcaggatga tctgctcgac ctcgcgcgcg atcgtctcgc cgccggcgac
      481 cgcgagcgcc gcttgcaggt agtgcgcctt gccgaacagg ctcatggact cgacgtgcgc
      541 gcgatagcgc tgtaagtccg cgagcgtgac cttgccgcgc ttggccagtg ccgcgagcgc
      601 gacggcgcgc accgtcgacg ccatgccgcg cgtgtagaag tcgggcgcga cgtcgcgctt
      661 caggaacgag tcgaggtact cgtgcagctt cgtctcgacg gcttccggta tcgcatggcc
      721 gtcctcgcgc agccaatcga atgcgagcgc cgtgtacgcg ctcaggtacg ggctcacgta
      781 ttgatcctgc ggcacgtagt acgtcatgcc gccgctcggc gcttggaagc tcgcggcttg
      841 ctccaatgtc gcctcgggaa gcgcggcggc ctcgggccac tcgagatccg gcggcaagta
      901 gtcgcgcagg tgcgtgaagt gcgacgccat gacgcccttc gtcaggcgtt gctcccagca
      961 gtagtacggg tagtcgcgca gataacggaa ggcgccgtcg atattgccga tgacgctcgg
     1021 gctcaaggtc acgccgacgt cgccggcgtc ggtgcgaatg cgctcgggga actcgagcga
     1081 ctccgtcgcg cgcgcgtcgt cgaacgtgcc gtacgtagcc ccaacctcga acgagcgccg
     1141 cttgtttacg acgagcgcgt gccgcaagcc gtcgccgtcc gtggcatcgc gcgccgtgac
     1201 ggtgaactcg atgcggcccg tcggcacgtc gcgcgtcgtc gcgacgcgcg cggcttgaac
     1261 cggcgccagc accgtcgtgc gcgcgtaagg ctcgagatgc accgtttgtg catgcgccac
     1321 gggcgccgcg acgccgtcgg cggccgcgat cgtcacgcga atgtcgcgcg cggcgtccgt
     1381 tcgattcatg acgctgaacg cggcgtcgaa gcggtcgcct tccgtcactt ggttcggcat
     1441 gacgggccgc acttcggtcg gcagatttac gcggaaggtc gcctcgccga gcccgaggcg
     1501 atcggtcggc gtggcagcca tgaccagcac gcgccagccc gtgagattgt cggggacctc
     1561 gagctcgaag ctgccgttgc cgcggctgtc gagctcgatc gacgggttcc agtagctcac
     1621 gtacttgaac accgatcgca tgctgaacgc cgctccgccg tcgccgccgg gatttgcgcc
     1681 cttcaagtcg atcttctgcc gcccgacgag ccgcgtcagc aagctgaagt tgcggacgtc
     1741 gagcccgtcc tgcgtgtaaa accccgcgta cggatcgaaa taccgcgtgc cgtcctgaat
     1801 cagatcgagc acggactcgt cgagcacggc gacggcgatc tcgatcggct cgcgctgctc
     1861 gcgttcgcgc ggccgcgctt gcacgcgcac tcggactttg tcgccgggct tgtagacggc
     1921 gcgatccgcg tgcgcctcga cgcgaatctc cttatatgga tctttgacgg gcacgtcgag
     1981 gtagccgagc ttgaacgacg gcttgccgag atcgagctcg ccctgctccg gcggcgggcc
     2041 ctcgacgcgc ggcgacatca cgagcaccga cagatagaaa cccggcatga agtccttttc
     2101 gacttcgaac tcgacgacgg gcgtgctgct gtcgaacgtc tgcacccact gcttcaagat
     2161 gccgtagcgc tcgacggtga ccaacgcgcg cgcgccggga tacgggtttt ttacaaggta
     2221 gcgcgccgtg tcgccgatct cgtaggtcga ctgctccggc accatctcga gcgcgtcgtc
     2281 gttaccgctg cgccacacga gctgaccctt gccgaggacc cacgtctgga gcaccgtctt
     2341 gtgcggcctg tcgcgcgtgt cgcggatcgt cgccgtgagc cggtagctgc cgggcgcctc
     2401 cggcgtaaac gtgcagttga gcggcgcgct cgacgacgtg ccgccgcact cgcctgccgc
     2461 gacccactcg tcgatgaact ccgtcagata cgccgtgccg gccccgcgga ctcgcgcggc
     2521 cttcgtctcg agccgctcga cgtcgatcgt gaccgccgta ccggctgccg gcgcaccgcg
     2581 gccgtcgacg acgaggtatt gcacggtggc aggctcgtcc tccttgaaca cccactccgt
     2641 gctgcgcagc ccgacgagcc ggtcgacggc gacgaattcc gcgctcgccg cgctcgcgac
     2701 gtagcggccg cggtcgtcgc gcacggcgcc ctccacgcta atcgtgccgt gcacgacttg
     2761 cgctcccaag tccgcgccga ggcggaacgt atgccgcgct tcgccctgcg catcgacttg
     2821 cgtttgctgc tgcgacagca cgagcgccgc cggctgctgc ataccggagt cgaattgaaa
     2881 acccgcggcc gcgggatggg cggtgcggaa cggccgtgcc gtgagctgcg ccgtgacgcg
     2941 cgcctcggcg tcgacatagg gcccgcccga gaacagcgtc gcgcgagtct cgacgccgac
     3001 gtcttggcct gcttcgaaca gatcgccgtt cagcgtcgtg cgcacgccga acggcgccgg
     3061 cgtgaaatcg cttacgagca cgcgcaacgg gaagcgcacg agcgcgtcgg cgtcgtagtc
     3121 gccgctcgca tttgcggacg ccgctgcgag cttcggcgtc aaacggaatt ggtaccagcc
     3181 gacggccgcc gattgcggga tcgcatagcg gccgctcagc gcgccgaact tcgacaacgt
     3241 cgcgtcggcg accgtgtgca cgacctggcc cgtcgggtcc acgatctcga gctggtacgg
     3301 ccccgtcggc gcgacgacga gcgactcgtt gtcctggtcg cgtacgtaga ttttccagtc
     3361 gatcgtgtcg ccggcgcggt agacgccctg tgccgtcgtg ccccaggcgt gcaaatgacc
     3421 gtactcgcgc tggctctgct cgaagaccgt gtagcgcgac gcgcggtagc tgtcgacggc
     3481 gaaccggtac tcgagcggca tgagcgccat gcccttcggc ccgtcgacgc gcacgaacaa
     3541 gcgctgacag ctctcggacc ggcccgcgtt gcagccgtag ccgttaccgg agagcgcgag
     3601 atcggcatcg agctcgcgcg ttccgggaaa cgtggcgacg ccggtcgcgt cggtcgccgc
     3661 ttgcgccttc ggcggcagag cggccgagag atcgcgcaag cgatcgacgt acaccgtgac
     3721 ggccgcatcg cggacaggct cgcccgtcgt gaggtccgtg acccagacca gcgagttgaa
     3781 gtgcccgagc ttgacgtgca attgatacgg cgtgacggcc gcgaacagcg gctcctccca
     3841 ccccgagcgc gccagggccg gactcgtctc gaggcggccg aagatcgcgc cggaccccgc
     3901 gccgagcacc gcgcgcacgc cgagcggcac gccgaattgc acgtccgcga tggcgggcag
     3961 ctcgcgcgaa agcacgaggt cggcgcgcgc tcccgccgga gtcagcgcgc gatagctcaa
     4021 cgagtagcgc tcgaggttcg tcacgtacag cggaacctcg gagtcgacgc cttgctcgag
     4081 cacggccgtc gtgtgcacga gcgtgtagtc gggcggccgg tgactcgtcg cgaacgcgaa
     4141 gtcgagcgga gcccgcaggc tgcggccgaa ctcgtccttc gggccctcgg ccgggtcgcg
     4201 cgtgcgcagc cgatacggct cggcggcttt gaggcgctcc ggcagccaca ccgtatacgg
     4261 cctgccgcgc tcgtgcggct gctcgagcag cgagtagtcg cgccaattgg cccacgggtc
     4321 gtagtccgtc cgtccgccgg cgaggtcggg cgtgagctcg atgtgatttt tgatttccga
     4381 gcccagcacg ggcgccgaga actgcaggcc gacgccgccg agcggattgc aggccccggc
     4441 gctgccgttg ctcgcgggca gcacgagctc gctgtcgtcg accgcgcggc aaacgacccc
     4501 gagaaactcg agctccggga acgtctggaa ctcgacggca acgcgattct cgtcgccgcg
     4561 ctcggtgccg accgccggct cgagacccgg ctccacgacc agctcgacgc gcgtgtcgag
     4621 cggcagctcg gcgctcggcc tgacgagcca gacgcggcgc gccgtctcgc cgcccgcgcc
     4681 ggtcggccgg tcgtcgacgc gcgccgtttg ccgctcgccg aaatcgacga acactcgctc
     4741 gccgggcaag cgcacgaagc gcggcacctc gcgctgctgc gtgtcggctt cgacggtcac
     4801 ggcggcacgc gcagcgccgc ggtggcgcat gaacaggtgc tcgcggaccg acgattcgct
     4861 gacggcctga gtgaacacgg cgcgaatcac cggcataccg gggctgcgcc acgtcgcaaa
     4921 gcccgggtag ctcagccgtg ggcgctccgt cgtgaactcg tggcgccgta ccccggccgt
     4981 cgtggcaccg tcctcggcca tgattccttc attgaccgtc aacgtgtagc gcgtcgcgag
     5041 cttcagctcg tcgccgtcgg cgagctgaca ggccagcgcg ctcgtgtcga gccagcgcca
     5101 ttggcacacc gtcggcggcg tgatcacgat cgggatttcg gcggccgtgc ggtccatgcg
     5161 cccgaccggc acgacggggc ggttgaactg cagaacgatc tgccgggcgg ccgggacgtt
     5221 cgtcccgcca ggcgtgatgt tcgtgatcgt cagcggcgca cgtgcgcctt gctgcgccgc
     5281 cgctacggtt ccgaggccga agaccaacac cgcgcgcagc agcgcgcgca ggcgcgccgc
     5341 gaaggcgccg cgatgttcca tgatgcgctc gaatcccacg ctctcaccct agcatcgtcg
     5401 cgatagtgac gatcactgta ggcgcgcgac atgacgaagg aagggcaaga atctgacgaa
     5461 tgatgatgct acgtcgggcg gtcgcgcagc agccgccgca ggatcttgcc cgacggcgac
     5521 ttcggaatcg cgtcgacgaa atcgacgcgg cgcaccttct tatagtgcgt cacgcaggcg
     5581 gcgacgtgcg ccatgagctc gtcggccgtc gccttgcctt tgagcacgac gaacgcgcgc
     5641 gggatctcgc cgcactcggc atccgtaagc ggcacgacgg ccgcgtcggc cacggccgga
     5701 tgcgccaaca gcgcagcctc gagctcggcg ggcggaacct gcatgccctt gtacttgatg
     5761 agctccttga gccggtcgac gacatagaaa tggccgtcgg catcggcgtg gccgatgtcg
     5821 cccgtgtgca gccagccgtc ggcgtcgacg gtcgcgcgcg tctcgtcggg gcgattcaag
     5881 taaccgcgca tgacttgcgg gccgcgcacc cagatctcgc cgtcgctgcc cggctcgacg
     5941 tcgcggccag tctcgatgtc gacgacgcgg cagtccgtgt tggcgacggg cgcgccgatc
     6001 gagcccagct tgcggcgcgc gacgtccgcc gtcgcaacgt gcgtcgcggg gctcgcctcg
     6061 gtcatgccgt agccctgctg cagcacgcag tcgatgcgcc gcatcgcctg ctccgtgatg
     6121 tcggcaccga gcggcgcggc gcccgagaac aacatcctga cgcgcgagaa atcgcgtccc
     6181 gccacggcgg ggtgcttcgc gaacgcgagc acgacgggcg gcacgacgtg cagcacggtc
     6241 gcgcggtggc gctcgaccag cgcgagatac ggctcgagct cgaacttcgg catgaccacg
     6301 accgtggcgc cggcccagag actcatgaag acgatgaccg tcaggccgta gatgtggaag
     6361 aacggcagga acgcgaccgt cgtgtcctcg ccgtcgttga aatgaccggc ggcatcgact
     6421 tgcagaatgt tcgcgacgag attcgcgtgc gtcagcatga cgcctttcgg caaccccgtc
     6481 gtgccgctcg agtacggcaa taagacgagg tcgtgcggct cgatcgtggc gcgcggcgcc
     6541 gtgccacccg cggccagcgt cgcgaaccga atcggcttgg ccgcgctgtg cggcgccgcg
     6601 gcggcggg
//
LOCUS       DATLEE010000133         4418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1635400, whole genome shotgun sequence.
ACCESSION   DATLEE010000133 DATLEE010000000
VERSION     DATLEE010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635400"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VM692_02870"
     CDS             <1..441
                     /locus_tag="VM692_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51135.1"
                     /translation="DALRQRIARLRGTLTFALRTEYHERLDVFAKHLRDLGKAIEVLQ
                     AQHVEFVRTRQAAVHSFEGYDTPISRLRTHVAESLARVNTLMARQGRVLELVAIDELV
                     ARRERLETYEDQARFALADSYDRATKERATAETAALQNITGGAP"
     gene            438..4064
                     /locus_tag="VM692_02875"
     CDS             438..4064
                     /locus_tag="VM692_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ51136.1"
                     /translation="MIRRRYLVLAMAPLFAAGCATAPESGTLASLRTVEPDVAEVEVA
                     DTLDLAMQSYRRYLAETPTSALTPEAMRRLADLQLEKEFGITGAAPTRRYTEMAAPES
                     AGGPSEIGAATAAAPVPTTIADAIAAAESDDEFERRTTGEAEFEPAAFDVTGSAGVSE
                     GGPLEAIKIYERLLTEYPNYERRDQVLYQMARAYDELGRTEEAMDVMQRLVGEFGYSR
                     YSDEVQFRRGEYYFTRRRFREAEAAYEQIVATSARSDFYELALYKLGWSLYKQDFYEE
                     ALHRYMALLDYKVSIGYDFDQAHAEEDERRVADTFRVISLSFSNLGGPDTLAEYYGTY
                     GNRAYEDRIYQNLAEFYFDKMRYADAAAVYDSFVDRYPFHRVSPDFSMRVIGIYEGGD
                     FPKLVVESKKSFATKYGLQSEYWQHFDAASRPEVLGYLKTNLEDLANHYHALYQEPAL
                     AEEKPANYAEALVWYRAFLASFPTEQESPGINYQLADLLLENDDFGEAAREYERTAYS
                     YAAHDRAAAAGYAAIFAHREQLKGAGAELETELKRATVESSLKFADTFPDHEHAAVVL
                     GAAAEDLYALEDYAPAATAARTLIERYPNAELPLRRTAWTVAAHSAFELHDYAVAEPA
                     YAQVLELTPPGDPAEDHERQALVDNLAASIYKQGEQANEAQDYRAAAGHFLRITQRAP
                     TSSIRPSAEYDAAAALMKLQDWTAAAAVLDGFRAAFPDHELNQEATKQLAFVYREGGE
                     TSLAAGEYERVAAEAADPELQREALLSAGELYEQAKNVDSALGVYERYVAEFQQPVEV
                     AIEIRSKVAEMYRVRGDDARYRDQLAALVAADAAAGGERTDRTRYLAAQAGLVLAEPR
                     YAAFNAVELVQPFQQNLARKRALMDEALKAYEDLVSYEVADVTTAATFHMAEIYASFS
                     RSLLDSERPTELNAAELAEYEDVLEEQAFPFEERAIEVHEANIDVMIAAGVYNKWVEQ
                     SFARLATLMPGRYAKEEQSIGQLGAIETFTYRAPNAPEPVAAAVPAEPERRTRAPAAK
                     GPTVRLEVLAGEGFTITDGGRVSPELRERYLAAVGLLEQGLYERGVAELEAVTAQAPE
                     LANPHVDLGIAYARTGRLVEAAASLEKAVALSASHPIAHEELALVYRKQARFGDARAQ
                     YEQALALYPDFHLANRNLAILCDLYQRDYGCALQHYQAYQTLVPEDAQVAIWIADLQG
                     RAGQ"
     gene            4074..4403
                     /locus_tag="VM692_02880"
     CDS             4074..4403
                     /locus_tag="VM692_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51137.1"
                     /translation="MPTKVLLVTVMLGTAAAAQAQQAPAAAQPTPPAGQRQAAAAPET
                     KSMSGMSILGNQEAPKSLVIVPWKSSEIGNGIGVSRALDTSIRAVDKDVFMRELKYYD
                     IREGRSK"
BASE COUNT          784 a         1510 c         1498 g          626 t
ORIGIN      
        1 gacgccttgc ggcagcgcat cgcgcgcttg cgaggcacgc tgacgttcgc gctgcgtacc
       61 gaataccacg agcggctcga tgtgttcgcg aagcaccttc gcgacctcgg taaagccatc
      121 gaagtattgc aggcgcagca cgtcgagttc gtgcgcacgc ggcaagcggc cgtgcacagc
      181 ttcgaaggct acgacacgcc gatcagccgg ctgcgcacgc acgtcgcgga gtcgctggcc
      241 cgggtcaata cgctgatggc gcgccagggg cgtgtgctcg agctcgtcgc gatcgacgag
      301 ctcgtcgcgc ggcgcgagcg cctcgagact tacgaggatc aagcgcgctt cgcgctcgcc
      361 gacagctacg accgcgcaac gaaggaacgc gccacggccg agacggcggc gctgcagaac
      421 atcacggggg gcgcgccgtg attcgccggc gctaccttgt gctcgcgatg gcgccgctgt
      481 tcgcggcggg ctgcgcgacc gcgcccgaga gcggcacgct ggcgtcgttg cgcaccgtcg
      541 agcccgacgt cgccgaggtc gaggtcgccg acacgctcga tctcgcgatg cagagctacc
      601 ggcgctatct cgccgagacg ccgacgagcg ccttgacgcc ggaggcgatg cgccgcctcg
      661 cggacctgca gctcgagaag gagttcggca tcacgggcgc cgcgccgacg cggcgttaca
      721 ccgagatggc agcacccgag agcgctggcg ggccgagcga gatcggtgcc gcgacggctg
      781 cggccccggt gccgacgacc atcgccgatg caatcgccgc cgccgaaagc gacgacgagt
      841 tcgagcgccg cacgaccggc gaagccgagt tcgagccggc cgcgttcgat gtcacgggaa
      901 gcgccggcgt ttccgagggc ggcccgctcg aggccatcaa gatctacgag cgcctgctga
      961 cggaataccc gaactacgag cgccgcgacc aggtgctgta ccagatggcg cgcgcctacg
     1021 acgagctcgg ccgcaccgaa gaggcgatgg acgtcatgca gcgcctcgtc ggggagttcg
     1081 gctactcgcg ctacagcgat gaagtgcagt tccgccgcgg cgagtactac ttcacgcgcc
     1141 ggcgcttccg tgaagcggaa gccgcgtacg agcagatcgt cgcgacaagc gcgcgctccg
     1201 acttctacga gctcgcgctc tacaagctcg gctggtcgct ctacaagcag gacttctacg
     1261 aggaagcgct gcaccgctac atggcgctgc tcgactacaa ggtctcgatc ggctacgact
     1321 tcgaccaagc tcacgcggaa gaggacgagc gccgcgtcgc ggacacattc cgcgtcatca
     1381 gcttgagctt ctcgaacttg ggcggccccg acacgctggc cgagtactac ggcacgtacg
     1441 gcaatcgcgc ctacgaagac cgcatctatc agaacttggc cgagttctac ttcgacaaga
     1501 tgcgctacgc ggatgccgcg gccgtctacg actcgttcgt cgaccgctat ccgtttcacc
     1561 gcgtgtcgcc cgacttcagc atgcgcgtca tcggcatcta cgagggcggc gactttccga
     1621 agctcgtcgt cgagtcgaag aaatcgttcg cgacgaagta cggcttgcag agcgagtact
     1681 ggcagcactt cgacgcggcc tcgcgtcccg aggtgctggg ttacctgaag acgaacctcg
     1741 aggacctcgc gaaccactat cacgcgctct atcaagagcc cgcactcgcg gaagagaagc
     1801 ccgcgaacta cgccgaggcg ctcgtctggt atcgcgcgtt cctcgcgtcg ttcccgacgg
     1861 agcaggagag cccaggtatc aactaccagc tcgcggacct cttgctcgag aacgacgact
     1921 tcggcgaagc cgcgcgcgag tacgagcgca cggcgtacag ctatgcggcg cacgaccgcg
     1981 cagccgcagc cggctacgcg gcgatcttcg cgcatcgcga gcagctgaag ggcgccggcg
     2041 cggagctcga gacggagctc aagcgcgcca cggtcgaaag ctcgctgaag ttcgccgaca
     2101 cgttcccgga ccatgagcac gcggccgtcg tgctcggcgc cgcggccgaa gatctctacg
     2161 cgctcgagga ttacgcgccg gccgcgacgg ccgcgcgcac cttgatcgaa cgctatccga
     2221 acgcggagct cccgctgcgc cgcacggcgt ggacggtcgc tgcgcactcg gcgttcgagc
     2281 tgcacgacta cgcagtggcc gagcccgcgt acgcgcaggt gctcgagctg acgccgcccg
     2341 gggacccggc agaagaccac gagcgtcagg cgctcgtcga caacctcgcc gcgtcgatct
     2401 acaagcaggg cgagcaggcc aacgaggcgc aggactaccg cgccgccgcg ggccacttcc
     2461 tacgcatcac gcagcgcgca ccgacgtcgt ccattcggcc ctcggccgag tacgacgccg
     2521 cggcggccct catgaagctg caggactgga cggctgcggc agcggtcctc gacggcttcc
     2581 gtgcggcgtt ccccgatcac gagctgaacc aagaggcgac gaaacagctc gcgttcgtct
     2641 accgcgaggg cggcgagacc tcgctcgcgg ccggcgaata cgagcgcgtc gcggccgaag
     2701 ctgccgaccc cgagctgcag cgcgaggcgt tgctgtcggc cggcgagctc tacgagcagg
     2761 ccaagaacgt cgacagcgcg ctcggcgtct acgagcgtta cgtcgcggag ttccagcagc
     2821 ccgtcgaagt cgcgatcgag atccgttcga aggtcgccga gatgtatcgc gtgcgcggcg
     2881 acgacgcgcg ctaccgcgac cagctcgccg cgctcgtcgc ggccgatgcc gcggccggcg
     2941 gcgagcgcac ggatcgcacg cgttatctcg ccgcgcaggc cggcctcgtg ctcgcggagc
     3001 cgcgctacgc ggcgttcaac gccgtcgagc tcgtgcagcc gttccagcag aacctcgcgc
     3061 gcaagcgcgc gctgatggac gaagcgctga aggcgtacga ggacctcgtc agttacgagg
     3121 tcgccgacgt cacgacggcc gccacgttcc acatggccga gatctacgcg agcttcagcc
     3181 gctcgctgct cgactccgag cggccgacgg agctcaacgc cgccgagctc gccgagtacg
     3241 aagacgtgct ggaagagcag gcgtttccgt tcgaggagcg cgcgatcgag gttcacgagg
     3301 cgaacatcga cgtcatgatc gccgccggcg tctacaacaa gtgggtcgag cagagcttcg
     3361 cgcggctcgc gacgctgatg ccggggcgct atgcgaagga agagcagagc atcggtcagc
     3421 tcggcgccat cgagactttc acgtaccgcg cgccgaacgc gcccgagccc gttgccgccg
     3481 ccgtgccggc cgagcccgag cgccgcacgc gcgcgccggc cgccaaaggg ccgacggtgc
     3541 ggctcgaagt gctcgcgggc gagggcttca cgatcacgga cggcggccgc gtgagcccgg
     3601 agctgcgcga gcgctatttg gctgcggtcg gtttgctcga gcaaggcttg tacgagcgcg
     3661 gcgtcgccga gctcgaggcc gtgacggcgc aggcgccgga gctcgcgaac ccgcacgtgg
     3721 atttaggtat tgcgtacgcg cgcacgggcc gcctggtcga agccgcggca agcctcgaga
     3781 aggccgtcgc tctgagcgcc tcgcatccga tcgcgcacga ggagctcgcg ctggtctacc
     3841 gcaagcaagc gcggttcggc gacgcgcgcg cgcaatatga gcaagcgctc gcgctgtacc
     3901 cggacttcca tctcgcgaac cgcaatctcg cgatcctctg cgatctttat caacgcgact
     3961 acggctgcgc gctgcagcac taccaggcgt atcagacgct cgtgcccgaa gacgcgcagg
     4021 tcgcgatctg gatcgccgat ttgcagggcc gtgcgggcca ataaggacga cacatgccga
     4081 cgaaagtact gctcgtgacg gtgatgctcg gtacggccgc agccgcgcag gcgcagcaag
     4141 cgcccgcggc ggcgcagccg acgccgcccg cggggcagcg ccaagccgcc gcggcgccgg
     4201 aaacgaagtc gatgtcgggc atgtcgatct tgggcaacca agaagcgccg aagtcgctcg
     4261 tcatcgtgcc gtggaagagc tccgagatcg gcaacgggat cggtgtgtcg cgtgcgctcg
     4321 atacgagcat tcgcgccgtg gacaaggacg tgttcatgcg cgagctcaag tactacgaca
     4381 tcagagaagg gcgctcgaag tagcgcattt tgtgaaat
//
LOCUS       DATLEE010000134         2352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_146263, whole genome shotgun sequence.
ACCESSION   DATLEE010000134 DATLEE010000000
VERSION     DATLEE010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146263"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VM692_02885"
     CDS             <1..337
                     /locus_tag="VM692_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ51138.1"
                     /translation="SAGFPAAAITNGGSVLGTIAALDKLIELTGPNTKVVPGHGPVVD
                     RAALVFHRDMAITVRDRIAKALREGKSADEIRALKPTAEFEQRVGGPANFITQVVDGL
                     ITELSAQRR"
     gene            354..1130
                     /locus_tag="VM692_02890"
     CDS             354..1130
                     /locus_tag="VM692_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HUQ51139.1"
                     /translation="MPRLLVFQHVAVEPLGTLDELIRARGHRIRFHNFERHPDAQPEV
                     DRYRGLIVLGGPMNVDDQERRPHLKTELEAIERALEQGKPVLGICLGAQLLAHVLGAP
                     VRRHSQAELGWYDLTVSSAGRNDPVLGVAGERLPVFQWHSYSYELPRDATHLASSATC
                     EQQAFRWGSNAYGFQFHLEADEAVIERWLQLPSFRAELAAAGLPTDEQAIRGATSELV
                     AATRQTAAAVFNNFLDLIGRPNRRRPLPSRGMPAWNETEP"
     gene            complement(1127..>2352)
                     /locus_tag="VM692_02895"
     CDS             complement(1127..>2352)
                     /locus_tag="VM692_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ51140.1"
                     /translation="VFGFFAFDQKLDSDPVHTEQAGAALYRFLWTAPNTPSAATPALF
                     DGYTSEINSELNTASAAVFGQLDWAVTDRLHLLPGVRFNYDEKQVDFDRRVAGGPAAP
                     TAAQLTQLLSVYRPQAFEADVNDSNVSGHLTLQLAATDRINTYATYATSFKSVGVNLG
                     GLPLDAANNTILATAEVEPEDVRHFEIGFKASPTPGTTANLTFFNTAIENFQTQVTNA
                     QFGVARGYLANADKVRVRGAEFDGNINIGDKFALRGSVSYTDAKYVSFTDAPPPLEGT
                     GGPAFVDASGGRLPGVSEWAASFGTEYRRVGNIFGKPGEFFTGLDIYYRGDFSSSPTP
                     SQYLNVDGYSLLNLRLGFRSDNGGWSGFLWSRNLLDEQYFEQLLAAPAGNGAGHYGAV
                     LGDERTYGATVRFSF"
BASE COUNT          414 a          781 c          773 g          384 t
ORIGIN      
        1 ctcggctggt tttcccgctg cagcgatcac caacggcggc agtgtgctcg gcacgatcgc
       61 cgcgctcgac aagctcatcg agctgacggg gccgaatacc aaagtggtgc ccggccatgg
      121 gcccgtcgtc gaccgcgccg cgctcgtgtt ccaccgcgac atggcgatca ccgttcgcga
      181 ccgtatcgcg aaagcgttgc gcgagggcaa gagcgccgac gagattcggg cattgaaacc
      241 cacagccgag ttcgagcagc gcgtcggcgg gccggcgaac ttcatcacgc aggtcgtcga
      301 cggcctcatc accgagctca gcgcgcaacg ccgctgacgt caccgacggc acgatgccgc
      361 gcctgctcgt ttttcagcac gtcgccgtcg agccgctagg aacactcgac gagctgatcc
      421 gcgcgcgcgg tcaccgaatt cggtttcaca acttcgagag gcatcccgac gcgcagcccg
      481 aggtcgaccg ctaccgcggc ctcatcgtgc tcggcggccc catgaacgtc gacgatcaag
      541 aacgacggcc gcatctgaaa accgagctcg aggcgatcga gcgcgcgctc gagcaaggca
      601 agcccgtgct cggcatctgt ctgggcgctc agctactggc ccacgtgctt ggcgcgccgg
      661 tgcgccgcca tagccaagcg gagctcggtt ggtacgatct cacggtcagc agcgcgggcc
      721 gcaatgatcc cgtgctcggc gtcgccggcg agcggctgcc cgtattccaa tggcattcgt
      781 attcgtacga gctgccgcgc gacgcgacgc accttgcgag ctcggcaacc tgcgagcaac
      841 aggcgtttcg ctgggggagc aatgcgtacg gctttcagtt tcatctcgaa gccgacgagg
      901 ccgtcatcga gcggtggctg cagctgccgt cgttccgtgc agagctcgcg gcggcaggac
      961 tgccgaccga cgagcaggcg atccgcgggg cgacttccga gctcgtcgcc gcgacgcggc
     1021 agacggccgc cgccgtgttc aacaactttc tcgatctcat aggccggcca aaccggcgac
     1081 ggcccttgcc gtcgcgcgga atgccggcct ggaacgagac cgagccctag aacgagaacc
     1141 ggaccgtcgc gccgtacgtg cgctcgtccc ctagcacggc accgtaatga ccggcgccgt
     1201 taccggcggg cgccgcgagc agctgctcga agtactgctc gtcgagcagg ttgcgcgacc
     1261 acaggaagcc ggaccagccg ccgttgtcgg agcggaagcc caggcgcagg ttcagcagcg
     1321 agtagccgtc gacgttcaag tattgcgacg gcgtcgggct cgacgagaag tcaccgcggt
     1381 agtaaatgtc gagacccgtg aagaactcac ccggcttgcc gaaaatgttg ccgacgcggc
     1441 gatactcggt cccgaacgac gcggcccact cggatacgcc gggcagccgg ccaccggacg
     1501 cgtcgacgaa cgccgggccg cctgtgccct cgagtggcgg cggcgcatcg gtgaacgaga
     1561 catatttggc gtcggtgtag ctgacggagc cacgcagcgc gaacttgtcg ccgatgttga
     1621 tattgccgtc gaactccgcg ccgcgcaccc gaaccttgtc ggcgttggcg aggtacccgc
     1681 gggcgacgcc gaactgcgcg ttggtcacct gcgtctggaa gttctcgatc gccgtgttga
     1741 agaaggtcag gttcgccgtc gtccccggcg tcgggctcgc cttgaaaccg atctcgaaat
     1801 ggcggacgtc ctcgggctcg acctcggcgg tcgcgagtat cgtgttgttc gctgcgtcga
     1861 gcggcagacc gcccaagttc acgccgacgg acttgaagct cgtcgcgtac gtcgcatagg
     1921 tgttgatgcg gtccgtcgcg gccagctgca gagtcagatg ccccgagacg ttcgaatcgt
     1981 tcacgtcggc ttcaaaagcc tgcggccgat agacgctgag cagctgcgtg agctgcgccg
     2041 ccgtcggcgc cgccggtccg cctgcgacgc ggcggtcgaa atcgacctgc ttttcgtcgt
     2101 agttgaagcg cacgccgggc agcaagtgca gccggtccgt gaccgcccag tcgagctggc
     2161 cgaacaccgc agcgctggcg gtattgagct cagaattgat ctcgctcgtg taaccgtcga
     2221 acaacgccgg cgtcgcggcc gaaggcgtgt tgggcgccgt ccacaggaac cgatacaacg
     2281 cagcacccgc ttgttcggtg tggactggat cgctgtcaag cttttgatcg aaggcaaaga
     2341 aaccgaatac tc
//
LOCUS       DATLEE010000135         4935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9082481, whole genome shotgun sequence.
ACCESSION   DATLEE010000135 DATLEE010000000
VERSION     DATLEE010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9082481"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VM692_02900"
     CDS             complement(<1..404)
                     /locus_tag="VM692_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51141.1"
                     /translation="MASEPLDFSSGSPTPDDGADVIPIIAPTVVAFIGRAERGPLNEP
                     VVIKGFEQFSRVFGGHTSFSFLSLAVQHFFWHGGETAVVVRVANRATRATLELAAGRE
                     VLRLQARQPGSREHLRVSVDYDRVERTPDKFNL"
     gene            complement(482..1150)
                     /locus_tag="VM692_02905"
     CDS             complement(482..1150)
                     /locus_tag="VM692_02905"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUQ51142.1"
                     /translation="MIPLRDDNPVRGIPVVTILTMLACMAVYLWQISLPPDGKQAAIT
                     LLGFMPALLFGHASIEGDPWVSPAGSIFTAMFLHGGFFHLAGNMIYLWIFGDNVEDRM
                     GRGRFIAFYLICGAIAALTQALPDTRSTVPMIGASGAVSGVLGAYIVLYPRANVLVAL
                     PFLLARVPALIALGVWFAAQLWSSLRAEPGAGGVAFMAHVGGFIGGALLIRWFVRDRR
                     KRTV"
     gene            complement(1190..2383)
                     /locus_tag="VM692_02910"
     CDS             complement(1190..2383)
                     /locus_tag="VM692_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HUQ51143.1"
                     /translation="MHRHHRAALLGSIHCAVAASLLTGCSLLTSPREPVAAEAAVVKP
                     ARPDPSAPRLAALETRHFELAPQQEIVGATQVLFARYENTFSAIGRQYNLGYEELRRA
                     NPGVDQWLPGEGTPIYLPTQTILPEAPHTGIVVNVPAMRLFYFTTDKGTTDKGTTDKS
                     TTAGAPGVTKVTTYPVGIGTEGWGTPFGEAKVVEKAKDPTWYVPASVRKEHAERGDPL
                     PSVVKPGPDNPLGAYAMTLSLPGYLIHGTNKPAGVGMRSSHGCIRLYPEDIEALFGRV
                     TRGTQVRLVNQPVLAAWQDGQLYLEVHPPLAEETHDLVAEADAALSRALMRAGAAATA
                     VVIDRAAIERIVTEQRGIPLPVLKQGRSLDEYLAGARIVENTVPVAVAEETARLAEPP
                     ATAGR"
     gene            complement(2467..3138)
                     /locus_tag="VM692_02915"
     CDS             complement(2467..3138)
                     /locus_tag="VM692_02915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUQ51144.1"
                     /translation="MTDSRWLRLTAAVFVLCSSLPARAGDDEGGARVAPFRQWSSAMS
                     RDEVQLGRIDADHDDGYLYRLPYGADVSFPIIQGYGAKLSHHGAERYTLDFGMPVGTP
                     VHAARDGLVVLVEDSHDSGCASEECGRDANFVVVLHADGTTGEYFHLERGSAQVRVGD
                     RVERGQPLARSGNTGFSTAPHLHFGVYRTDRDHRTESLAVSFETRGGAVRQPRSGALY
                     VNRTE"
     gene            complement(3221..4204)
                     /gene="sohB"
                     /locus_tag="VM692_02920"
     CDS             complement(3221..4204)
                     /gene="sohB"
                     /locus_tag="VM692_02920"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HUQ51145.1"
                     /translation="MSEFLAAYGLFLAQLATFVLLIIIAFVVISASRRRGHADGLVVD
                     HLNRRFEDTADELKLAIDGKGPYKKAVKARQKERKRQDKARAKETSSKPRMFVLDFKG
                     DLRASAAASLREEVSAVLRVAKAGEQVLVRLENSGGTVHEHGFAASQLTRLKQHNLKL
                     LVAVDKVAASGGYLMACVADRLYAAPFAIIGSIGVLAQLPNFHRLLEEKGVDYEQIMA
                     GRYKRTLTMFGKNTDEGRAKLQQEVEDIHELFKGQIVKHRPQVDVESVATGEHWYGIR
                     ALELKLVDEIKTSDDLLLDAAKDFDLYHLAYKRRRSFQERMLGGVDSLLTR"
     gene            complement(4210..>4935)
                     /locus_tag="VM692_02925"
     CDS             complement(4210..>4935)
                     /locus_tag="VM692_02925"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HUQ51146.1"
                     /translation="GTATIMLMGKVCTRACQFCAVDTGNPRGWLDPDEVENSARTVEL
                     MGLGYVVLTSVDRDDLPDGGARHYADCIRAIKARCPQTAVEALTPDFGGNFVDVATVV
                     DSGLDVFAQNIETVERLTHKVRDARAGYEQTLAVLAFAKRHKPAVLTKSSLMLGLGET
                     AEEIAAGLDDLRKASVDIVTFGQYLRPTPNHLAVERYVTPQEFELYRQWGLERGFLEV
                     VAGPLVRSSYRADRVFEKNNVGL"
BASE COUNT          822 a         1650 c         1662 g          801 t
ORIGIN      
        1 aggttgaact tgtcgggcgt acgctcgacg cggtcgtagt cgaccgacac gcgcaagtgc
       61 tcgcggctgc cgggctggcg cgcctgcaag cgcagcacct cgcggccggc cgcaagctcg
      121 agcgtcgcgc gcgtggcgcg attcgcgacg cgtacgacga cggccgtctc gccgccgtgc
      181 cagaagaagt gctgcacggc cagcgacaag aagctgaacg acgtatggcc gccgaacacg
      241 cggctgaact gttcgaaacc tttgatgacg acgggctcgt tcagcggccc tcgctcggca
      301 cgaccgatga acgctacgac ggtgggggcg atgatcggga tgacatcggc gccgtcgtcc
      361 ggagtcggcg agccggagct gaagtcgagg ggttcggacg ccattctgcc cgctttggga
      421 aaaacgcccg catcatagct ggcgccgcag gcgcttgcag cattggaagt gtcagcaaac
      481 gtcagacggt gcgcttacga cggtcgcgca cgaaccagcg gatcagcaac gcaccgccga
      541 tgaaaccgcc gacgtgggcc atgaacgcga cgccgcccgc tccgggctcg gcgcgcagcg
      601 agctccaaag ttgagccgcg aaccacacgc cgagcgcgat cagtgcaggc acgcgcgcca
      661 gcaagaacgg cagcgcgacg aggacgttcg cgcgcggata gagcacgatg taggcgccga
      721 gcactcccga gaccgcgccg ctcgccccga tcatcggcac cgtcgagcgc gtgtcgggca
      781 acgcttgcgt cagcgcggcg atggcgccgc agatcagata gaacgcgatg aagcgcccgc
      841 gccccattcg gtcctcgacg ttatcgccga agatccagag atagatcatg ttgccggcga
      901 ggtggaagaa cccgccgtgc aggaacatcg ccgtgaagat cgagcccgcc ggcgagaccc
      961 aggggtcgcc ttcgatgctc gcgtgaccga acagcagcgc gggcatgaag ccgagcagcg
     1021 tgatcgcggc ttgcttcccg tccggcggca acgagatttg ccacagatag acggccatgc
     1081 aggccagcat cgtcaggatc gtgacgacgg gaatgccgcg aaccggattg tcgtcgcgga
     1141 gcggaatcat ctctcaggcg ctgcgccgca gcgcggcgca agcggcgcct cagcggccgg
     1201 ccgtcgcggg cggctctgcg agccgcgccg tctcctcggc caccgcgacc ggcaccgtgt
     1261 tctcgacgat gcgcgcgccc gcgaggtact cgtccaacga gcggccctgc ttcagcaccg
     1321 gtagcgggat gccgcgttgc tcggtcacga tcctctcgat ggccgcgcgg tcgatgacga
     1381 cggctgtggc cgccgcaccg gcgcgcatga gcgcgcggct cagcgccgcg tcggcctcgg
     1441 ccacgagatc gtgcgtttcc tcggcgagcg gcggatgaac ctcgagatag agctggccat
     1501 cctgccacgc ggcgagcacg ggctggttca cgagccgtac ctgcgtgccg cgcgtgacgc
     1561 ggccgaacag cgcctcgata tcctcggggt agagccgaat gcagccgtgg ctcgaccgca
     1621 tgccgacgcc ggccggcttg ttcgtgccgt gaatcaagta gcccggcagc gacaacgtca
     1681 tcgcgtaagc gccgagcgga ttgtcgggac cgggtttgac gacgctcggc agcggatcgc
     1741 cgcgctcggc atgctctttg cggaccgacg ccggcacgta ccacgtcggg tccttggctt
     1801 tctcgacgac cttggcctcg ccgaacggcg tgccccagcc ctcggtgccg atgccgacgg
     1861 ggtacgtcgt gaccttcgta acgcccggcg cgccagccgt cgtggacttg tcggtcgtgc
     1921 ccttgtcagt cgtgcccttg tcagtcgtaa agtagaagag ccgcatggcc gggacgttga
     1981 cgacgatccc cgtgtgcggc gcctcgggca ggatcgtctg cgtcggcagg tagatcggcg
     2041 tgccctcgcc gggcagccat tgatcgacgc cgggattggc gcgccgcagc tcctcgtaac
     2101 ccaagttgta ctggcggccg atggccgaga acgtgttctc gtagcgcgcg aacagcacct
     2161 gcgtcgcgcc aacgatttcc tgctgcggcg cgagctcgaa atggcgtgtc tcgagcgcgg
     2221 ccagccgcgg cgccgaggga tccggtcgag cgggcttgac cacggctgcc tccgcggcga
     2281 cgggctcgcg cggtgacgtc agcagcgagc agccggtcag caacgaggcc gcaacggcgc
     2341 agtgaatcga cccgagcaat gcggcacggt gatggcgatg cataggcgtt gatgaccccg
     2401 agagagcttc ttgaggagta tacctgtccc gcgccgccga acgcccccgt cgccgagcag
     2461 cgacgactat tcggtgcggt tcacatacag cgcgccgcta cgcggctgac ggaccgcgcc
     2521 gccgcgggtc tcgaagctca cggccagcga ctcggtccga tggtcgcggt ccgtgcgata
     2581 gacgccaaag tgcagatgcg gcgccgtgct gaaccccgtg ttgcccgagc gcgcgagcgg
     2641 ttggccgcgc tcgacacgat cgccgacgcg cacttgcgcg ctgccgcgct cgagatggaa
     2701 gtactcgccc gtcgtgccgt cggcgtgcaa gacgacgacg aaattcgcgt cgcggccgca
     2761 ctcttcgctc gcgcagcccg agtcgtgcga gtcctcgacg agcacgacta ggccgtcgcg
     2821 agccgcatgc acgggtgtgc cgaccggcat gccgaagtcg agcgtatagc gctcagcgcc
     2881 gtgatggctc agtttcgcgc cgtagccttg gatgatcggg aagctcacgt cagcgccgta
     2941 cggcaaacgg tagaggtaac cgtcgtcgtg gtctgcatcg atgcggccga gctgcacttc
     3001 gtcgcggctc atcgccgacg accattggcg gaacggcgcg acgcgcgcgc cgccttcatc
     3061 gtcgccagct cgtgccggca ggctcgagca aagcacgaaa actgcggccg tgagccgcaa
     3121 ccagcgggag tccgtcatcc tcgttcttat tgttagcggc ggatgtgcgc gcagtatctc
     3181 aggccgctcg cgcagcgggc ccgagttgtt acataaaccg tcagcgcgtg agcaagctgt
     3241 ccacgccgcc gagcatgcgt tcctggaagc tgcgccggcg cttgtacgcg aggtggtaga
     3301 ggtcgaaatc tttggcggcg tcgagcagca agtcgtcgct cgtcttaatc tcgtcgacga
     3361 gcttcagctc gagcgcgcga atgccgtacc aatgctcgcc cgtcgctacg ctttcgacgt
     3421 cgacttgcgg ccggtgcttg acaatctggc ctttgaacag ctcgtggatg tcctcgactt
     3481 cctgctgcag cttcgcgcgg ccctcgtcgg tattcttgcc gaacatcgtc agcgtgcgct
     3541 tgtagcggcc ggccatgatc tgctcgtagt cgacgccctt ctcttcgagc agccgatgaa
     3601 aattcggcag ctgcgccagc acgccgatcg agccgatgat agcgaacggc gccgcgtaca
     3661 accggtccgc gacgcaggcc atcaggtaac cgccgctggc cgcgactttg tcgacggcca
     3721 cgagcagctt caagttgtgc tgcttcaggc gtgtcagctg cgacgcggcg aagccgtgct
     3781 cgtgcaccgt gccgccggag ttctcgagcc gcacgaggac ctgctcgccg gctttggcga
     3841 cgcgcagcac ggccgagacc tcctcgcgca gcgaggccgc ggcgctcgcg cgcagatcgc
     3901 ctttgaaatc gagcacgaac atgcgcggct tggagctcgt ctctttcgcg cgcgctttgt
     3961 cctggcgctt gcgctctttc tgccgcgcct tgacggcttt cttgtaaggc cccttgccgt
     4021 cgatcgcgag cttgagctcg tcggcggtgt cctcgaagcg ccgattcaga tgatcgacga
     4081 cgagaccgtc ggcatggccg cggcgccgcg acgcactgat gacgacgaaa gcgatgatga
     4141 tcaacagaac gaaggtcgcg agctgcgcga ggaacaggcc gtacgcggca aggaactcgc
     4201 tcacttagcc taaagaccca cgttgttctt ttcgaacacg cggtcggcgc gatagctcga
     4261 gcgcacgagc gggcccgcga cgacctcgag gaagccgcgc tcgagccccc actggcgata
     4321 gagctcgaac tcctgtgggg tcacgtagcg ctcgacggcc aagtgatttg gcgtcgggcg
     4381 cagatattgg ccgaacgtca cgatgtcgac gcttgccttc cgcaaatcgt cgagcccggc
     4441 cgcgatctcc tcggccgtct cgcccaagcc gagcatgagg ctcgacttcg tcagcacggc
     4501 cggcttgtgc cgctttgcga aggcgagcac ggccagtgtt tgctcgtagc cggcccgcgc
     4561 gtcgcgcacc ttgtgcgtca gccgctcgac cgtctcgatg ttctgcgcga agacgtcgag
     4621 gcccgagtcg acgaccgtcg cgacgtcgac gaagttgccg ccgaagtccg gcgtcagcgc
     4681 ctcgacggcc gtctgcgggc agcgggcttt gatcgcgcgg atgcagtcgg cgtaatgacg
     4741 cgcgccgccg tcgggtaaat cgtcgcgatc gacggacgtc aacacgacgt agccgagccc
     4801 catgagctcg acggtgcgcg ccgagttctc gacctcgtcg ggatcgagcc agccgcgcgg
     4861 gttgcccgtg tcgacggcgc agaactggca tgcacgcgtg cagaccttgc ccatcaacat
     4921 gatcgtcgcc gtgcc
//
LOCUS       DATLEE010000136         7462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4614362, whole genome shotgun sequence.
ACCESSION   DATLEE010000136 DATLEE010000000
VERSION     DATLEE010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4614362"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VM692_02930"
     CDS             <1..582
                     /locus_tag="VM692_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HUQ51147.1"
                     /translation="WPMIEFECDDALATAAARFGADAAVERVVVCSPDKDLTQCVQGD
                     RIVCFDRMRRRMLDERGVCEKFGVYPKSIPDWLALVGDNADGYPGIPKWGAKSAAAVL
                     GKYGTLEAIPTDAAAWAVNVRGAAALAATFTTHRTDAFLFRRLATLRTDVPLTESLED
                     LRWRGPNVAALTALAAVWDDTVIVERATRLASS"
     gene            complement(579..1283)
                     /locus_tag="VM692_02935"
     CDS             complement(579..1283)
                     /locus_tag="VM692_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUQ51148.1"
                     /translation="MATFVLVHGAWHGSWCWKRVRKALQAEGHDVFTPTLTGVAERSH
                     LTSPQVTLDTHVADIVNLIRWEELTDAVLCGHSYGGCVITGVADRIPERLRALVYLDA
                     FILENGQSLHDVLPREVGEAQVRGANETGDGWRVPPITAEFFRVNLDDRAWVDRQCTP
                     QPLATFSQRLRLDKTFPAAKCTHILATGYEHSPFPPFHELAKRKGWKSLTVDCGHDVM
                     LDKPNELAAMLRDAAR"
     gene            1366..2685
                     /locus_tag="VM692_02940"
     CDS             1366..2685
                     /locus_tag="VM692_02940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HUQ51149.1"
                     /translation="MSLRRCISLGSVVVACSALAQTTPATAPVLDARASDPAAMGWMV
                     GSPPPPDKVVRFADDSWMHFPQQRWAFSHVRELMPTAVVARGDGPVSELPRALRSDID
                     AVTFKPIGRDTTMTWAQSLDANYTDGVLILHRGRVVYERYFGVLTPERQHIAFSVTKS
                     FVATIAATLIDEGVLDANATIARYVPELAKSGFGDATIRQVLDMTTGLAYTEDYNDPQ
                     SPVWEMTRAGGFRARPQGYRGPQSFFEYVAGIAKAGEHGKVFVYKTPNTDVLAAVLRR
                     VTGKSLSALLQERIFGRLGAEHDAYFTVDSTGAEFAGGGLNLTLRDLARFGEAMRLKG
                     RYNGQQIVPQRVVEDIERGASREQFAPAGYATLPGWSYHTMWWISHNAHGAYTARGIH
                     GQGIYIDPAAEMVIARFASHPLAGNVNLDPTSLPAYEAVAQRLMATK"
     gene            2778..3434
                     /locus_tag="VM692_02945"
     CDS             2778..3434
                     /locus_tag="VM692_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HUQ51150.1"
                     /translation="MRTTVISAMLVLAGAFGTAQAQAPAPVAGTDYIEISNGRPLDPA
                     EGGVVVVEEFFNYICPACNSFEPLFVAWAAKLPEGVKLVHVPASFRADFEQYAKAYYA
                     AESLNIAEKSHAAVYSAIHRGRTLPAEGQRPDEEKIAKFYSDYGVDPQQFLAVMRSFA
                     VNLKVKRAAEHMTRARIPSTPSVVINGRYLVQGNTYQDMLRTADYLIAKERAAATSGG
                     "
     gene            complement(3445..5172)
                     /locus_tag="VM692_02950"
     CDS             complement(3445..5172)
                     /locus_tag="VM692_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51151.1"
                     /translation="MQRSRLVAFAAALSIHAAAMAAERDDFVPVTDRMLERPDAGDWL
                     NWRRTQNGWAFSPLTQIDRRNVAKLEQVWTQPMGTGAQEATPLVHAGVMYVPNRGDYI
                     QAFDAATGTLRWEYHRQLPEGVNGGTNRSLAIYGTNLIDAGSDNQIYAVDARTGTLVW
                     ETPVHGATVRARATSGPLIAGGKVITGRQCQPDATFEGCVVTAHDAVTGKELWRTHTI
                     PQPGEPNFDSWGDVPMEQRWHVGTWMVPSYDPALKRIFVGTSVTIPAPKFILGGADKQ
                     HLYHNSTLALDLETGKIVWYYQHLIDHWDLDHPFERLLVDTAVAPDASEVSWINPRIR
                     PGERRRVVTGIPGKTGVVYTLDRETGEFLWARPTIFQNVIANIDGATGKVTDDPERVY
                     TRKDESIMVCPGMNGGKNWPAGAYSPRTNAMYMPMQNQCMNARTQTDERDPKLVYGLE
                     QQQVLSPGATGQGTIWAVSAETGKVLWKHEQRAGVMSLVATAGGLVFGGDVGGNFTAY
                     DEETGKVLWQTNVGAAISGYPVTYTAGGKQYVAVTTGSSGVYNNGRRFAPESVPEQTD
                     SAVFVFALP"
     gene            complement(5317..6891)
                     /locus_tag="VM692_02955"
     CDS             complement(5317..6891)
                     /locus_tag="VM692_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HUQ51152.1"
                     /translation="MASNSDAAKGENIGAVRCDRPQNGAEYLESLRDGREIYIYGDRV
                     DDVTTHPAFRNTARMTARLFDALHDKEKSKKLLLPTDTDGKGKTHAFFRAPKTVEESI
                     AGRDAIAEWQRITYGWMGRSPDYKAAFLGTLGGNAEFYAPFEANAKRWYTFCQERVPF
                     VNHAIIHPPVDRDKPPDQVADVCVHVEKETDGGLIVSGAKVVATGSVLTNYTFIAHHG
                     LIPLQDKSFALVFMLPTNAPGVKFLCRTSYEMTAGVMSSPFDQPLSSRLDENDAVFIM
                     DKVFVPWENVFVYGDIDKANNFFPRTGFLPRFVVHGCTRLAVKLDFIAGLLLKAVEAS
                     GTKDYRGVQANVGEVLAWRNMFWALTEAMVRDPKPWKGPYLLPNPDPGGAYQVLATIA
                     YSKIKYLIEQTVASGLIYLNSHARDFANPEIRPYIDKYMRGSNGYTAEQRIKLMKLLW
                     DCLGTEFGARHELYEINYGGSTEEIRRYVLFGAMGNGNAAKFKGFAEQCMEEYDLNGW
                     KAPDLINPDDLSFHKK"
     gene            complement(6928..>7462)
                     /locus_tag="VM692_02960"
     CDS             complement(6928..>7462)
                     /locus_tag="VM692_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51153.1"
                     /translation="QEQPPPFDAWPTDTLFMPQKDVFFNGEAVQVLYQPAAHTDGDTM
                     VFFRRSDVVATGDVFTTTAYPVIRPEDGGGIDGIVAALNRIIELTVPREKQEGGTLVI
                     PGHGRLCDEADVVEYRDMVTIIRDRIKSMIEMGMTLRQVQAAKPTADYDGRYGATSGF
                     WTTEQFVAAAYESLKND"
BASE COUNT         1210 a         2433 c         2449 g         1370 t
ORIGIN      
        1 tggccgatga tcgagttcga gtgcgacgat gcgctcgcga cggccgccgc gcgtttcggc
       61 gcggatgcgg ccgtcgagcg cgtcgtcgtg tgctcgccgg ataaggacct cacgcagtgc
      121 gtgcaaggcg accgcatcgt ctgcttcgac cgcatgcggc gcagaatgct cgacgagcgc
      181 ggcgtctgcg agaagttcgg cgtctatccg aagtcgattc ccgactggct cgcgctcgtc
      241 ggcgacaacg ccgacggtta tcccggcatt ccgaagtggg gcgctaagtc cgcggccgcg
      301 gtgctcggca agtacggaac gctcgaggcg ataccgacgg acgccgcggc gtgggccgtc
      361 aacgtacgcg gtgccgcggc gctggccgcg acgttcacga cgcaccgaac ggacgctttc
      421 ctattccgca ggctcgcgac gctgcgcacc gacgtgccgc ttacggagtc gctcgaggac
      481 ttgcgctggc ggggtccgaa cgtcgcggcg ttgacggcgc tggccgccgt ttgggacgac
      541 accgtgatcg tcgagcgcgc gacgcgcctc gcgagtagtt agcgagccgc gtcgcgcagc
      601 atcgccgcga gctcgttcgg cttgtcgagc atgacgtcgt ggccgcagtc gacggtcagc
      661 gacttccaac ctttgcgttt ggcgagctcg tggaacggtg ggaacggcga gtgctcgtag
      721 cccgtcgcga ggatgtgcgt gcacttggcg gcggggaatg tcttgtcgag ccgcaagcgt
      781 tgactgaacg tcgcgagcgg ctgcggcgtg cattggcggt cgacccaagc gcgatcgtcg
      841 agattgacgc gaaagaactc cgccgtgatc ggcggcacgc gccagccgtc acccgtttcg
      901 ttggctccgc gcacttgagc ctcaccgacc tcgcgcggca ggacgtcatg cagactttgg
      961 ccattctcga ggatgaatgc gtcgaggtac acgagcgcgc gaagccgctc cggtatccgg
     1021 tccgcaactc ccgtgatgac gcaaccgccg tacgaatggc cgcagagcac ggcgtccgtg
     1081 agctcttccc agcggatcag gttgacgatg tcggcgacat gcgtgtcgag agtcacttgc
     1141 ggcgaggtca ggtgcgagcg ctcggcgact cccgtcagcg tcggcgtgaa gacgtcgtga
     1201 ccttccgctt gcagtgcctt acggacgcgc ttccagcacc agctgccatg ccatgcgcca
     1261 tgcacgagaa cgaacgtcgc cattgcaatc cccctgcgga aacgacggct catcataatc
     1321 gcagcggcgt gctactgttc tgccgccgac gcttcagggg tggtcatgag cttgcgacgt
     1381 tgcatctcac tgggttccgt ggtggtcgca tgctcggcgc ttgcccagac gacgcccgcg
     1441 accgctcccg tgctcgacgc gcgcgccagc gatcccgccg cgatgggctg gatggtcggc
     1501 tcgccgccgc cgccggacaa ggtcgtgcgg ttcgccgacg acagctggat gcattttccg
     1561 cagcagcgtt gggcgttctc gcacgtgcgc gagctcatgc cgacggccgt cgtcgcgcgc
     1621 ggcgacggtc ccgtgtcgga gttgccgcgc gcgctgcgct cggacatcga cgccgtgacg
     1681 ttcaagccca tcggccgcga tacgacgatg acgtgggcgc agtcgctcga cgcgaattac
     1741 accgacggag ttctgatcct gcatcgcggc cgcgtcgtct acgagcgtta cttcggcgtg
     1801 ttgacgccgg agcgccagca catcgcgttc tccgtaacga agtccttcgt cgcgacgatt
     1861 gcggcgacgc tgatcgacga gggcgtactc gacgcgaacg cgaccattgc acgctatgtg
     1921 ccggagctcg cgaagagcgg cttcggcgat gcgacgatcc ggcaggttct cgacatgacg
     1981 acgggcctcg cctacacgga ggactacaac gatccgcagt cgcccgtctg ggaaatgacg
     2041 cgcgccggcg ggtttcgtgc gcggccgcag ggctaccgag gcccgcagtc gttcttcgag
     2101 tacgtcgccg gcatcgccaa agccggcgag cacggcaagg tgttcgtcta caagactccc
     2161 aacaccgacg tgctcgctgc agtgctgcgg cgcgtcaccg gtaaatcgct gagcgcgttg
     2221 ctgcaggagc gaatcttcgg ccggctcggc gccgagcacg acgcgtattt caccgtcgac
     2281 tcgacgggcg cggagttcgc cggcggcggc ttgaatctga cgctgcgcga cctcgcgcgc
     2341 ttcggcgagg cgatgcggct caaagggcgt tacaacggcc aacaaatcgt gccccagcgc
     2401 gtcgtcgagg acatcgagcg cggcgcgagc cgcgaacagt tcgcgccggc gggctacgcg
     2461 acgctgcccg gctggagcta tcacactatg tggtggatct cgcacaacgc gcacggcgca
     2521 tatacggctc gcggcatcca cgggcagggc atctacatcg atccggccgc ggagatggtc
     2581 attgcacgct tcgcttcgca tccgctcgca gggaacgtca atctcgatcc gacgtcgctg
     2641 cctgcctacg aggccgtcgc gcagcgcctc atggcgacaa agtaaaataa ggaaaacttc
     2701 gtgatatcgg cactatgggt gccggccgat gtcgtaagct tgcagccctc agggcaccgt
     2761 tgatgaggac cgctaccatg cgaactaccg tcatatccgc catgctcgtt cttgccggcg
     2821 ctttcggcac ggctcaggcg caagccccgg cgccggtggc cggcacggac tacatcgaga
     2881 tttccaacgg tcgtccgctc gatcccgccg aaggcggcgt ggtcgtcgtc gaagagttct
     2941 tcaactacat ttgccccgct tgtaactcgt tcgagccgct attcgtcgcg tgggccgcga
     3001 agctgccgga aggcgtcaag ctcgtgcacg tgcccgcgag ctttcgcgcg gatttcgagc
     3061 agtacgcgaa ggcctactat gcggcggaga gcctgaacat cgccgagaag tcgcacgcag
     3121 ccgtgtactc ggccatccac agaggccgca cgctgccggc cgaaggccaa cggccggacg
     3181 aggaaaagat tgcgaagttc tactctgact acggcgtcga tccgcaacag ttcttggccg
     3241 tcatgcgtag ctttgccgtg aacttgaagg tgaaacgtgc ggcggagcac atgacgcgcg
     3301 cgcgcattcc gagcacgccg tcggtcgtca tcaacggccg ctatctcgtc cagggcaaca
     3361 cgtatcagga catgctgcgg acggccgact acctgatcgc gaaggagcgg gcggcggcaa
     3421 cctcgggcgg ctaacgccgc tcgctcaggg caaagcgaac acgaacacgg cgctgtcggt
     3481 ttgctcgggc acggactcgg gcgcgaaccg ccgcccgttg ttgtagacgc ccgaagagcc
     3541 cgtcgtgacc gccacgtact gcttgccacc ggccgtgtag gtgacgggat agccgctgat
     3601 cgccgcgccg acgttcgttt gccacaacac cttgccggtc tcttcgtcgt acgccgtgaa
     3661 gttgccgccg acgtcgccgc cgaacacgag cccgcccgcc gtcgcgacca acgacatgac
     3721 gccggcgcgc tgctcgtgct tccagagcac cttgccggtc tcggccgaca cggcccaaat
     3781 cgtgccttga cccgtggcgc caggtgagag cacttgctgc tgctccaagc cgtagacgag
     3841 cttcgggtcg cgctcgtcgg tctgcgtgcg cgcgttcatg cactggtttt gcatcggcat
     3901 atacatggcg ttggtgcgcg ggctgtaagc gccggccggc cagttcttac cgccgttcat
     3961 gccgggacaa accatgatcg attcgtcttt gcgcgtatag acgcgctccg ggtcgtccgt
     4021 caccttgcct gtagcgccgt cgatgttggc aatgacgttc tggaagatcg tcggccgtgc
     4081 ccacaggaac tcgccggtct cgcgatcgag cgtgtagacg acgccggtct tgccgggaat
     4141 gccggtgacg acccggcgac gctcgccagg tcggatacgc gggttgatcc acgacacctc
     4201 gctcgcgtcc ggcgcgacgg ccgtgtcgac gagcaagcgc tcgaacggat gatcgagatc
     4261 ccagtggtcg atcaggtgct ggtaatacca aacgatcttg ccggtctcca agtcgagcgc
     4321 gagcgtcgag ttgtgataaa ggtgctgctt gtctgcgccg cccaggatga acttcggcgc
     4381 ggggatcgtg accgacgtgc cgacgaagat gcgcttgagc gccgggtcgt agctcgggac
     4441 catccacgtg ccgacatgcc agcgctgctc catcggcacg tcgccccagc tatcgaaatt
     4501 cggctcaccc ggctgcggaa tcgtgtgcgt gcgccacagc tccttgcctg tgacggcgtc
     4561 gtgcgccgtc acgacgcagc cctcgaacgt cgcgtcgggc tggcactgcc ggcccgtgat
     4621 gactttgccg ccggcgatca gcgggcccga cgtcgcgcgc gcacgcaccg tggcgccatg
     4681 taccggcgtt tcccaaacga gcgtgcccgt gcgcgcgtcg accgcgtaga tctggttgtc
     4741 gctgccggcg tcgatgaggt tcgtgccgta gatcgcgagg ctacgattcg tcccgccgtt
     4801 cacgccttcg gggagctgac ggtgatactc ccagcgcagc gtgccggtgg ccgcgtcgaa
     4861 cgcctggatg tagtcgccgc ggtttggcac gtacatgact ccggcgtgca cgagcggcgt
     4921 cgcttcctgt gcgcccgtgc ccatcggctg tgtccaaact tgctcgagct tcgcgacgtt
     4981 gcggcggtcg atctgggtca gcgggctgaa cgcccagccg ttctgtgtgc gccgccaatt
     5041 gagccagtcg ccggcgtccg ggcgctcgag catcctgtcc gtgacgggaa cgaaatcgtc
     5101 gcgttcggcc gccatggctg cggcgtggat gctgagcgcg gccgcgaagg ccacgagtcg
     5161 agatcgctgc atggatgtcc cccaaacgaa gcccgcagcc atggtagtgc gaacgtgggg
     5221 tcgccgtcat ggcgaacgtg cgtgcgccgc gcgcgcattt ccccgcggag ccgggttcgc
     5281 ccaagggggg tggggcgccc ggctccgcgg ggcgggctac ttcttgtgga acgacaggtc
     5341 gtcgggattg atgaggtccg gcgccttcca gccgttcaag tcgtactctt ccatgcactg
     5401 ctcggcgaag cccttgaact tggcggcgtt gccgttgccc atcgcaccga acagtacgta
     5461 gcggcggatc tcctcggtgc tgccgccgta gttgatctcg tagagctcgt gccgcgcccc
     5521 gaactccgtg cccaagcagt cccagagcag cttcatgagc ttgatgcgct gctcggccgt
     5581 atagccgttc gaaccgcgca tgtacttgtc gatgtacggg cggatctccg ggttcgcaaa
     5641 gtcgcgcgcg tgcgagttca gataaatcaa gcccgacgcg accgtttgct cgatcaagta
     5701 tttgatcttc gagtacgcga tcgtcgcgag cacttgatac gcgccgcccg ggtccggatt
     5761 cggcagaagg tacgggcctt tccacggttt cgggtcgcgg accatcgcct ccgttagcgc
     5821 ccaaaacatg ttgcgccagg ccagcacttc gccgacgttg gcctggacgc cacgatagtc
     5881 cttcgtgccc gaagcctcga cggccttgag cagcaagccc gcgatgaagt cgagcttcac
     5941 ggcaagccgc gtgcagccgt gcacgacgaa ccgcggcagg aagcccgtac gcgggaagaa
     6001 attgttggcc ttgtcgatgt cgccgtagac gaacacgttc tcccacggca cgaacacctt
     6061 gtccatgatg aacacggcgt cgttctcgtc gagacgcgac gacaacggct gatcgaacgg
     6121 gctcgacatg acgccggccg tcatctcata cgaggtgcgg cacaggaatt tcacgcccgg
     6181 cgcgttcgtc ggcagcatga acacaagcgc gaagctcttg tcttgcagcg ggataaggcc
     6241 gtggtgcgcg atgaacgtgt agttcgtcag cacggagccc gtcgcgacga ctttggcgcc
     6301 cgagacgatc aagccgccgt cggtctcctt ctcgacgtgc acgcagacgt cggcgacttg
     6361 atccggcggc ttgtcgcggt cgacaggcgg atgaatgatc gcgtggttca cgaacggcac
     6421 gcgctcttgg cagaacgtgt accaacgctt cgcgttggct tcgaacggcg cgtagaactc
     6481 ggcgttaccg ccgagcgtgc ccaagaacgc ggccttgtag tccggcgagc ggcccatcca
     6541 gccgtaggtg atgcgctgcc actcggcgat cgcgtcgcgg ccggcgatcg actcctcgac
     6601 ggtcttcggg gcgcggaaga acgcgtgcgt cttgcccttg ccgtcggtgt ccgtcggcag
     6661 cagcagcttc ttggattttt ccttgtcgtg cagtgcgtcg aacaaccgcg ccgtcatgcg
     6721 cgccgtgttg cggaaggcag gatgcgtcgt gacatcgtcg acccggtcgc cgtagatgta
     6781 gatctcgcgg ccgtcgcgca gcgattccaa gtactccgcg ccgttctgcg ggcggtcgca
     6841 gcgcacggcg ccgatgttct cgccctttgc ggcgtcgctg ttgctcgcca tcctcgttgc
     6901 catgaatcgc tcctgtcgct ctagacgtta atcgttcttc aaactttcat acgcggccgc
     6961 gacgaactgc tccgtcgtcc agaacccgct ggtcgcgccg tagcgcccat cgtaatccgc
     7021 ggttggcttg gccgcttgga cctgacgcag cgtcattccc atttcgatca tgcttttgat
     7081 gcggtcacga atgatcgtga ccatgtcgcg gtactcgacg acgtcggctt cgtcgcagag
     7141 cctgccgtga cccgggatca cgagcgtgcc gccttcttgt ttctcgcgcg gcaccgtcag
     7201 ctcgatgatg cggttcagcg ccgcgacgat accgtcgatg ccgccgccgt cctcggggcg
     7261 gatcacggga tacgccgtcg tcgtgaagac gtcgcccgtg gcgacgacgt ccgagcggcg
     7321 gaagaacacc atcgtgtcgc cgtcggtgtg cgcggccggc tgatacaaca cttgcacggc
     7381 ttcgccgttg aagaacacgt ccttctgcgg catgaacaag gtatcggtcg gccaggcgtc
     7441 gaacggcggc ggctgttcct gc
//
LOCUS       DATLEE010000137         2658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8271430, whole genome shotgun sequence.
ACCESSION   DATLEE010000137 DATLEE010000000
VERSION     DATLEE010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8271430"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /gene="prs"
                     /locus_tag="VM692_02965"
     CDS             <1..567
                     /gene="prs"
                     /locus_tag="VM692_02965"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HUQ51154.1"
                     /translation="VDVHADQITGFFDIPVDNVYASPVLLGDAWKQKYKDIVVVSPDV
                     GGVERARALAKRLDDAGLALIDKRRPQPNMAEVMNVIGEISGKTCVLIDDMVDTAGTL
                     CQAASVLKQRGAVRVVAYITHPVLSGPAVERIKTSALDEIVVTDTIPLSETARACPQI
                     RQLSVAELLAETLRRINESDSVSSLYLD"
     gene            650..1396
                     /locus_tag="VM692_02970"
     CDS             650..1396
                     /locus_tag="VM692_02970"
                     /inference="COORDINATES: protein motif:HMM:NF004130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HUQ51155.1"
                     /translation="MKAAFELAGEPRAQLGRTDSRRLRKAGKVPAIMYGGGEAPEALI
                     LDHNALKNQMNREAFFTSILTLKIGSKAQQVVVKSVERHPARPLIMHLDFQRVREDVE
                     ITITVRIHFTGEAESKGVKEQGGVVDHLLTDVEIRCLPRYLPEYLTLDVSALELNQIY
                     HLSDIKLPEGVTIVALAGGHDHPVVAVNLPRAEEIDIPAETATPAGEVPATAQVEPAK
                     EGEAAAGADAKADAKAGAKPEAKGKAEGKK"
     gene            1540..2148
                     /gene="pth"
                     /locus_tag="VM692_02975"
     CDS             1540..2148
                     /gene="pth"
                     /locus_tag="VM692_02975"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HUQ51156.1"
                     /translation="MGTPIELIVGLGNPGAEYAATRHNAGFWFVDLLAGEHRARFASQ
                     RKLHGEAAEITVTGNRIRLLKPMTYMNLSGQSVGAAVNYFKIPTEHVLVAYDELDLPT
                     GRVQLRFDGGSAGHNGIGNVIEHIGPKFWRLRFGIGHPRDKEQPGGRKDVIDHVLERA
                     GGPEETALRDKLREAAELLPMLLEHGAERVKNRLHTRKPESE"
     gene            2152..>2658
                     /locus_tag="VM692_02980"
     CDS             2152..>2658
                     /locus_tag="VM692_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HUQ51157.1"
                     /translation="MGIKCGIVGLPNVGKSTLFNALTAAAIPAENYPFCTIDPNVGVV
                     TVPDPKLDALAAIVKPEKVVPTVVEFVDIAGLVEGASQGQGLGNQFLAHIRETHAIAH
                     LVRCFVNDDIVHVSGKINPLHDIEIIDTELMLADLDSVQKAHQRAEKAAKTNEKTAVA
                     RRDALKKLL"
BASE COUNT          526 a          885 c          845 g          402 t
ORIGIN      
        1 gtcgacgtgc acgccgatca gatcacgggc ttcttcgaca ttcccgtcga caacgtctac
       61 gcgtcgccgg tgttgctcgg cgacgcctgg aaacagaagt acaaggacat cgtcgtggta
      121 tcgcccgacg tcggcggcgt cgagcgcgcg cgcgccctcg cgaagcggct cgacgatgcg
      181 ggcctcgccc tcatcgacaa acggcgcccg caaccgaaca tggccgaggt catgaacgtc
      241 atcggcgaga tctcgggcaa gacctgcgtg ctgatcgacg acatggtcga taccgccggc
      301 acgctgtgcc aggcggcctc cgtattgaag cagcgtggcg ccgttcgggt cgtggcctat
      361 atcacccacc ccgtcctgtc cgggcctgcc gtagagcgca taaagacctc ggcgctcgac
      421 gagatcgtgg tgaccgacac catccccttg agcgagacgg cgcgggcctg tccccaaatc
      481 cgccagctgt ccgtggccga gctcctggcg gaaacgctgc gccgcatcaa cgagtccgac
      541 tcggtcagct ccctctactt ggattagaag acgcagtata aaaacagggc gactagcccg
      601 gccttgggag gccctggtat gatgcgcggc ttcctggagg tacccacctg tgaaagccgc
      661 attcgaactg gccggagagc cacgcgcgca gctcggtcgc accgatagcc gccgcttgcg
      721 caaggccggc aaagtccccg ccatcatgta tggcggcggc gaagcgcccg aggcgctaat
      781 cctcgaccac aacgcgctca agaaccagat gaaccgtgaa gccttcttca cgagcattct
      841 gacgctgaag atcggctcga aggcgcaaca ggtcgtcgtc aaaagcgttg agcgccatcc
      901 cgcgcggccg ttgatcatgc acctcgactt ccagcgcgtc cgtgaggacg tcgagatcac
      961 gatcacggta cgcattcact tcacgggcga agccgagtcg aagggcgtca aggagcaagg
     1021 cggcgtcgtc gatcacttgc tgaccgacgt cgagatccgc tgcttgccgc gctacctgcc
     1081 cgagtacctc acgctcgacg tgtcggcact cgagctgaat cagatctatc acctgtcgga
     1141 catcaagctg cccgaaggcg tcacgatcgt cgcgctcgca ggcggccacg atcaccccgt
     1201 cgtcgcggtc aatctgccgc gcgccgagga gatcgacatt ccggccgaga cggcaacgcc
     1261 ggccggcgaa gtgccggcga cggcgcaagt cgagcccgcg aaggaaggcg aagcggccgc
     1321 cggcgccgac gcgaaagccg acgccaaggc cggcgccaag cccgaggcga agggcaaggc
     1381 cgaaggcaag aagtagcacg cgacttttcg ttcgcgcact cgagaggccg ccttcgggcg
     1441 gcctttcagc tccagaggtg ccggtgttac ggtttcggtt caaccgacgc gaccgacgac
     1501 gcagcgctct cgcgaaccga aaccgcaaca ccggcacgta tgggcacgcc aatcgagttg
     1561 atcgtcggcc tgggcaatcc cggcgccgaa tacgcggcga cccgccacaa cgcggggttc
     1621 tggttcgtcg acctgctcgc cggcgagcac cgcgcgcggt tcgcgtcgca acgcaagctg
     1681 cacggcgaag cggccgagat cacggtcacc ggcaaccgca tccgcttgct gaagccgatg
     1741 acgtacatga acttgagcgg ccagtcggtc ggcgcggccg tcaactactt caagattccg
     1801 accgagcacg tgctcgtcgc ttatgacgag ctcgatctac cgacgggtcg cgtacagttg
     1861 cgcttcgacg gcggcagcgc cggtcacaac ggcatcggca acgtcatcga gcacatcggc
     1921 ccgaagtttt ggcggctgcg cttcggcatc ggccacccgc gcgacaagga gcagccaggc
     1981 ggccgcaagg acgtcatcga tcacgtgctc gagcgcgccg gcgggcccga agaaaccgcg
     2041 ctgcgcgaca agttgcgcga ggcggccgag ctcttgccga tgctgctcga gcacggcgcc
     2101 gaacgcgtca agaatcgctt gcacacacgc aagccggaga gcgaataagc catgggtatc
     2161 aagtgtggca tcgtcggatt gccgaacgtc ggtaagtcga cgctgttcaa cgcgctgacg
     2221 gccgcggcga ttccggccga aaactatccg ttctgcacga tcgatccgaa cgtcggcgtc
     2281 gtgacggtgc cggacccgaa gctcgacgcg ctggccgcga tcgtcaagcc cgagaaggtc
     2341 gtgccgaccg tcgtggagtt cgtcgacatc gcgggactcg tcgaaggcgc gtcgcaaggt
     2401 caagggctcg ggaatcagtt tctcgcgcac atccgcgaga cgcatgcgat cgcgcacctc
     2461 gtgcgctgct tcgtcaatga cgacatcgtg cacgtctcgg gcaagatcaa tccgctgcac
     2521 gacatcgaga tcatcgacac cgagctcatg ctcgccgact tggactccgt gcagaaggcg
     2581 caccagcgag ccgagaaagc ggcgaagacc aacgagaaga cggcggtcgc gcgccgcgac
     2641 gcgctgaaga agctgctc
//
LOCUS       DATLEE010000138         2344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6037308, whole genome shotgun sequence.
ACCESSION   DATLEE010000138 DATLEE010000000
VERSION     DATLEE010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6037308"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1342)
                     /locus_tag="VM692_02985"
     CDS             complement(80..1342)
                     /locus_tag="VM692_02985"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HUQ51158.1"
                     /translation="MSRAPAESSQSGPLAGLTVLDLTLALAGPFATFLLAGLGARVIK
                     IENPAAPDHCRENAPFIGRNGVSLGRTASDDVSVAALNRLRGKYAVTLNLKQPGSREV
                     FADLVRQADVVVENFAPGTLDRLGVGYEFARERNPRIVYCSISGYGADHSTTGSGKAM
                     DSIVQALSGLMMTSGAPDVPPVRVGVPFADLSAPIFAVVGVLAALRQRDVTGVGRHVD
                     VSMLGVLTSLVAAEPFDLLEACGVPQRTGRMVPRLTPFGVYESADGHLAICAPTDQFA
                     RGVFQAIGQPELEQDPRFATRDARVANKESINQRIETFTRSRATAELVPLFERHGVPA
                     APVRTPAAAVRDPHVLARGETVRLEHPELGRVADLIGPGVPIRFAGTAPTATRPAPAV
                     GQDNALVYGDWLGYSDETIERLRAARNI"
     gene            complement(1384..2259)
                     /locus_tag="VM692_02990"
     CDS             complement(1384..2259)
                     /locus_tag="VM692_02990"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HUQ51159.1"
                     /translation="MRAAARLRTAQPSLSQQIRQLEKSVGAKLLDRSRQHVGLTNAGR
                     IFLQQAKDIVARVEHAGRLAKQAADGRAGELSVGTFPSADVRIVPALRPLVAEHMPDL
                     RLILHSKYAVEPIAGLQSGTLDIAFVRGPLEADGLESVELLRERLVIVLPSHHALARR
                     KTIPVERLDDLPCITMERKLSPPLHDAAADLYREARIRMHAVSKADNVLGHLKLVQEG
                     LGFALLPDSISALLPPGVTFRPLDCDPVPSVSVLVAWKAGNGSRLVRELVELAQRCSQ
                     ASKDAPRLTQRATKS"
BASE COUNT          393 a          764 c          804 g          383 t
ORIGIN      
        1 cgcatcgttt cccagcgtcc aaaacggctc ctcgaaaccg gcgcgaagct cgtcgatggc
       61 gctcgcgctc atggtccgct caaatgtttc gagcggcgcg cagccgctcg atggtttcgt
      121 cgctgtaacc gagccagtcc ccgtacacga gcgcgttgtc ttggccgact gcgggtgcgg
      181 gccgcgtcgc tgtcggcgca gtgcccgcga agcgaatcgg cacgccgggg ccaatcaggt
      241 ccgcgacgcg cccgagctcc gggtgctcga gccgcacggt ctcgccgcgc gcgagcacgt
      301 gcggatcgcg gacggcagcc gccggcgtgc gcacgggagc ggccgggacg ccgtgccgct
      361 cgaatagcgg aacgagttcc gccgtggcgc ggctgcgagt gaacgtctcg atgcgctgat
      421 tgatggattc cttgttggcg actcgcgcgt cccgcgtggc gaaacgcgga tcctgctcga
      481 gctcgggctg cccgatcgct tgaaagacgc cgcgcgcaaa ttggtccgtc ggggcgcaga
      541 tcgcaagatg gccgtcggcc gactcgtata cgccgaatgg cgtcagccgc ggaaccatgc
      601 gcccagtgcg ttgcggcacg ccgcacgctt cgagcaagtc gaacggttcc gccgcgacca
      661 gcgacgtgag cacgccgagc atcgagacat cgacgtgccg gccaactccc gtgacgtcgc
      721 gctgccttag cgccgcgagc acacccacga cggcgaagat cggggccgac agatcggcga
      781 acggcacgcc gacgcgcacg ggcggcacgt cgggcgcgcc ggaggtcatc atgaggccgc
      841 tcagcgcttg gacgatgctg tccatcgctt tgccggagcc cgtcgtcgag tggtcggcgc
      901 cgtaccccga gatcgagcag tacacgatgc gtgggtttcg ctcacgcgca aattcatacc
      961 cgacaccgag gcggtcgagc gtgcccggcg cgaagttctc gacgacgacg tccgcctgac
     1021 gcacgagatc ggcgaacacc tcccgcgatc ccggctgctt caggttcagt gtcaccgcgt
     1081 acttgcctcg caagcgattc agggccgcca cggagacgtc gtcggatgcg gtacggccga
     1141 gcgagacgcc gttccgtccg atgaaggggg cattctcgcg gcaatgatcg ggcgcggcgg
     1201 gattctctat cttgatgaca cgggcgccga ggccggcaag caggaatgtt gcgaacgggc
     1261 cggccaacgc cagcgtcaag tcgagcacgg tcagtccggc caacggcccg gactggctgc
     1321 tttctgcggg tgcgcggctc ataggtgcgg tgcccgcagt ctactgagtc gatggcgctc
     1381 ccgctaagac ttcgtagcgc gctgcgttag acgtggcgcg tctttcgatg cttggctaca
     1441 gcgctgcgcg agctcgacga gctcgcgcac gagtctcgag ccgttgccgg ccttccaggc
     1501 gacgagcacc gacacgctcg ggacgggatc gcagtcgagg ggccgaaacg ttacgccggg
     1561 cggcagcaac gcgctaatcg agtcgggcag cagcgcgaac cctaatcctt cctgcacgag
     1621 cttgagatgg ccgaggacgt tgtcggcttt ggacacggca tgcatgcgaa ttcttgcttc
     1681 gcggtaaaga tcggccgccg catcgtgcaa tgggggcgac agcttccgct ccatcgtgat
     1741 gcacggcaga tcgtcgaggc gttcgaccgg gatggttttg cgccgagcga gcgcatggtg
     1801 cgacggcaac acgatgacga gtcgctcgcg cagcagctcc accgattcga ggccatccgc
     1861 ctcgagcggc cctcgcacaa acgcgatgtc gagcgtgccg gactgaaggc ccgcgatcgg
     1921 ctcgacggcg tacttgctgt gcaggatcag acgcagatcg ggcatgtgtt cggctacgag
     1981 tggccgcaga gcggggacga tccgcacgtc cgccgatgga aacgtgccga ccgaaagctc
     2041 gccggcgcgg ccatccgccg cctgcttcgc cagccgcccc gcgtgctcga cgcgcgcgac
     2101 gatgtccttg gcctgctgca gaaagatgcg cccggcattc gtcagtccga cgtgctgccg
     2161 gctgcgatcg agcaatttcg ctccgaccga tttctcgagt tgacggatct gttggctcag
     2221 ggaaggttga gccgtgcgca agcgtgccgc tgcgcgcatg aagctgagct cttcggcgac
     2281 cgcgatgaag taacgaagat gtctgaattc cacgcgtgcg gttggggtag tcgacggaag
     2341 ctca
//
LOCUS       DATLEE010000139         3549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3057896, whole genome shotgun sequence.
ACCESSION   DATLEE010000139 DATLEE010000000
VERSION     DATLEE010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057896"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1551
                     /locus_tag="VM692_02995"
     CDS             217..1551
                     /locus_tag="VM692_02995"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HUQ51160.1"
                     /translation="MQRATALIAAAAAFVTTTTVIKGQTAPKQFQTKGQPDVEGHPAV
                     DDNGRPVLPPGAKDAATGYDNKTNGFLSQGPSFDSLNEDTVVPLRSFNDNRFLFEEVE
                     VVADGLGPVYNAQSCGECHQNVATGGASQIAEHRTGRMKDGAFFESAGGSLVHSRAID
                     PDIVEVVNFEDDIRTFRISTNTLGAGYVEAISNSTLLKIRQEQPAAMRGSTVIVPVLE
                     ADKRPRLGRFGWKSQHASLESFAADAYLNEMGITSPLFPDENTSSGTNVGHDTDYDPV
                     PDPEDDGVDVEAFADFMRSTKAPARGPIDANVLAGEAIFKDIGCAVCHVPTIVTAPPG
                     TKINGGDLTVPEALGSKVIHPYSDFMLHNIDTGDEIPVQPTADYAYTAPQIRTAPLWA
                     LRTRNRLMHDGLTFTEREAIERHGGQATAVRERYRALGADGQQLLLKFLESL"
     gene            complement(1631..2782)
                     /locus_tag="VM692_03000"
     CDS             complement(1631..2782)
                     /locus_tag="VM692_03000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUQ51161.1"
                     /translation="MSVELAEIRDAVTRLCADFPGRYWRDKDRARAYPQEFVEALSAA
                     GYLAVLIPEEFGGSGLGVGAASAVLEEIHKSGCNGAACHAQMYTMGTVLKHGSDAQKR
                     AYLPQIAAGTLRLQAFGVTEPTSGTDTTRIRTTAVRDGDVYRVNGQKVFISRTEHSDL
                     MLLLARTSAREQVAKPTDGLSVFLVDLRESVGRGLTIKPVATMMNHATTELFFDNLVV
                     PAANLVGVEGKGFGYILDSMNAERILIASECIGDARWFIAKATAYAKERKVFGRTIGE
                     NQGIQFPIARAHVQTEAAALMVQRAAALFDAGEKCGAEANMAKMLAADASKEAGDVCI
                     QTHGGYGFAEEYDIERKFRETRLYQVAPVSTNLILSYVGTHVLGMPKSF"
     gene            complement(2779..3462)
                     /locus_tag="VM692_03005"
     CDS             complement(2779..3462)
                     /locus_tag="VM692_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HUQ51162.1"
                     /translation="MKRALRALLAVLLMVSPLSRAQQPATAPDPGDRFAVAYVETRAD
                     SAAAGRAALERYREAVRAEAGCLGVELFEQAGRRGRFAIVESWRDATTFDARAAEPKR
                     QLLEALRPIRISDYDERPYKSLTLDARTAAGGVAVVSHVDVAPNTQAPELLRTLAERS
                     RGEQGNARFVVLQHVQRANHFTVVEQWQSDAALDAHVAAAHTREYRDALQPLTGSPLD
                     ERVYTPISR"
BASE COUNT          635 a         1206 c         1136 g          572 t
ORIGIN      
        1 gaggcgcatg ctgcacgcgt accgcttgtt aattttccga agtcgggagt aaacaaactc
       61 ccactggggc ttacgcttgc gcgatgcacg cgtatcatgg gcgtcgccgc gcaagcggcg
      121 ctcgggcgaa cgttgcgagc acggtcagga cgaccggcat gcacgccgcc cgagtcgact
      181 cgactacaag gacgaagctt caactggagg gatccaatgc agagagccac cgcgctcatc
      241 gcggcagcgg ccgcgtttgt cacgacgact accgtaatca aagggcaaac agcccccaag
      301 cagtttcaaa ccaagggcca acccgacgtc gagggtcacc ctgccgtcga cgacaacggc
      361 cgtccggttt tgccgccggg cgcgaaagac gcggcgacgg gctacgacaa caagaccaac
      421 ggcttcctga gccaaggacc gtcgttcgat agcctcaacg aagacaccgt cgtgccgctg
      481 cgctccttca acgacaaccg tttcctcttc gaggaggtcg aagtcgtcgc ggatggactc
      541 ggacccgtct acaacgcgca gagctgcggc gagtgccatc agaacgtggc cacgggcggc
      601 gccagccaaa tcgccgagca tcgtaccggc cgcatgaaag acggcgcgtt cttcgagtcg
      661 gccggcggct cactcgtgca ctcgcgcgcc atcgatcccg acatcgtcga ggtcgtcaac
      721 ttcgaagacg acatccgcac gttccgcatt tcgacgaaca cgttgggggc gggttacgtc
      781 gaagcgatct cgaactcgac cttgctcaag atccgtcagg agcagcccgc ggccatgcgc
      841 ggctcgaccg tgatcgtgcc cgtgctcgaa gcagacaagc ggccgcgtct tggccgtttc
      901 ggttggaaga gtcagcacgc gagcctcgag tccttcgcgg ccgacgcgta tctcaacgag
      961 atgggcatta cgagcccgtt gttccccgat gagaacacct cgagcggtac caacgtcggc
     1021 cacgacacgg attacgaccc ggtgccggac cccgaggacg acggagtcga cgtggaagcg
     1081 ttcgcggact tcatgcgctc tacgaaggcc cccgcgcgcg ggccgatcga cgccaacgtc
     1141 ttggccggcg aggcgatctt caaggacatc ggctgcgcgg tttgtcacgt gccgacgatc
     1201 gtcacggcgc cgcccggcac gaagatcaac ggcggcgatc tcaccgtccc ggaagcgctg
     1261 gggagcaagg tcatccaccc ctacagcgac ttcatgctgc acaacatcga caccggggac
     1321 gaaatccccg tgcagccgac cgctgactac gcatacacgg cgccgcagat ccgcacggcg
     1381 ccgttgtggg cgttacgaac gcgcaaccgg ctcatgcacg acgggctcac gttcacggag
     1441 cgcgaagcca tcgagcggca cggtgggcaa gcgacggcgg tcagggagcg atacagagca
     1501 ctcggcgccg acggccaaca actgctgctg aagttcttag agtcgctgta acacgggcgg
     1561 gtttggacgc cgcgccgtga gcggcggcgg cgccaagccc atccgtaagc gcggcgcttt
     1621 cgcagcgccc tcagaaggac ttcggcatcc cgagcacgtg cgtgccgacg tacgacagga
     1681 tcaggttcgt cgacaccggc gcgacctgat acagccgcgt ctcgcggaac ttgcgctcga
     1741 tgtcgtattc ctcggcgaag ccgtagccgc cgtgcgtttg gatgcagacg tcgccggcct
     1801 ccttcgaggc gtcggccgcg agcatcttgg ccatgttggc ttcggcgccg cacttctcgc
     1861 cggcgtcgaa caacgccgca gctcgttgca ccatcaacgc ggcggcctcg gtctgcacgt
     1921 gcgcacgcgc aatcggaaac tggattcctt ggttctcgcc gatcgtccgg ccgaacacct
     1981 tgcgctcttt cgcatacgcg gttgccttcg cgatgaacca gcgcgcgtcg ccgatgcact
     2041 cggacgcgat caaaatccgc tcggcgttca tgctgtccag gatgtagccg aagcctttgc
     2101 cctcgacgcc gaccagatta gcggccggca caacgagatt gtcgaagaag agctccgtcg
     2161 tcgcgtgatt catcatcgtc gcgaccggct tgatcgtcag cccacggccg acggactcgc
     2221 gtagatcgac gaggaacacc gataagccgt ccgtcggctt cgcgacttgc tcgcgcgcgc
     2281 tcgtgcgcgc gagcagcagc atgagatccg aatgctcggt gcgcgagatg aataccttct
     2341 ggccgttgac gcgatagacg tcgccgtcgc gcacggccgt cgtgcgaatg cgcgtcgtgt
     2401 cggtgccgct agtcggctcc gtcacgccga acgcttgcag tcgtagcgtg ccggccgcga
     2461 tctgcggcag gtacgcgcgt ttctgggcgt cactcccgtg cttcaaaacc gtgcccatcg
     2521 tgtacatctg cgcgtggcag gccgcgccgt tgcagccgct cttgtgtatc tcctcgagca
     2581 cggcgctcgc cgcaccgaca cccaagcccg agccgccgaa ctcctcgggt atcagcacgg
     2641 cgaggtagcc ggctgcggac aacgcttcga cgaactcttg cggataggcg cgtgcgcgat
     2701 ccttgtcgcg ccaataacgg cccggaaagt cggcgcatag gcgcgtgacg gcgtcgcgaa
     2761 tctcggctag ctccacgctc atcgtgagat cggcgtgtag acgcgctcgt cgagcgggct
     2821 gcccgtcagc ggctgcaacg cgtcgcgata ctcgcgcgtg tgcgcggccg cgacgtgcgc
     2881 gtcgagcgcc gcatcgcttt gccactgctc gacgaccgtg aagtggttgg cgcgctgcac
     2941 gtgctgcagc acgacgaacc gcgcgttgcc ttgctcgccg cggctccgct cggcaagcgt
     3001 ccgcagcaac tcgggcgcct gcgtgtttgg cgcgacgtcg acgtgcgaaa cgacggccac
     3061 gccgccggca gccgttcgtg catcgagcgt caacgacttg taaggccgct cgtcgtagtc
     3121 gctgatgcgg atcggccgca gcgcctcgag cagctgccgc ttcggctcgg ccgcgcgagc
     3181 gtcgaatgtc gtagcgtcgc gccacgattc gacgatagcg aaacggccgc ggcgacccgc
     3241 ctgctcgaac agctcgacgc cgaggcagcc ggcctcggcg cgcacagcct cgcgataccg
     3301 ctcgagcgcc gcccgccccg ctgccgccga atcggctcgc gtctcgacgt aggcgaccgc
     3361 gaagcgatcg cccgggtcgg gcgcggtggc gggctgctgc gcgcgactca gaggcgacac
     3421 catcagcagg acggccaaca gggcgcgcag ggctcgcttc atcaatgcga tttgatccaa
     3481 ttgatcacgg ccgagcgatc cgtgcgatac gcgaagtccg tgccgcggca cagcgcgttc
     3541 aagccgaac
//
LOCUS       DATLEE010000140         4064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9086234, whole genome shotgun sequence.
ACCESSION   DATLEE010000140 DATLEE010000000
VERSION     DATLEE010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9086234"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..629
                     /locus_tag="VM692_03010"
     CDS             156..629
                     /locus_tag="VM692_03010"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HUQ51163.1"
                     /translation="MDFFVWLESTPIAIFIRDYRFAYATIESGHYVGLSLLVGGIMLI
                     DLRVLGMARVLPLKNMIGLLPIVWIGFIINVLTGSLLFIYGATNFGFNRAFHLKMAFM
                     VVAGLNAFAFDLAVRRSGGDWVVADRPPPIVKGFATASLVFWLCVVTTGRWMAYL"
     gene            691..1008
                     /locus_tag="VM692_03015"
     CDS             691..1008
                     /locus_tag="VM692_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002540963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HUQ51164.1"
                     /translation="MSDKPTAAEQTELEAAVLRRLLRHFDEHKDVQNIDLMILAGFCR
                     NCLSKWMVEAANERGLEVGLDEARERIYGMPYSDWKERFQPPATEEQLARLAAVEKAK
                     KNK"
     gene            complement(1025..2044)
                     /gene="ispB"
                     /locus_tag="VM692_03020"
     CDS             complement(1025..2044)
                     /gene="ispB"
                     /locus_tag="VM692_03020"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HUQ51165.1"
                     /translation="MRQNRNMSLAKTNPDLPSLEALRALVGDDWNDVNRTIVRRLGSD
                     VALVNQVAHHIIHNGGKRLRPLAVLLAARACGAGSSQDHITAAAIIEFIHTATLLHDD
                     VVDSSGLRRGRETANAVFGNQASVLVGDFLYSRSFQMMVELEEIRILDVLANATNTIA
                     EGEVLQLMNCNNPDISEADYMEVIYRKTAKLFEAGTRIGAILAKQDDAVEQALAAYGR
                     HLGQAFQLVDDALDYDAKVEELGKNIGDDLAEGKPTLPLIYAMAHGSAAERHMIREAV
                     ETGGTRNLTSIQLAIETSGGLRYTAERARAEVELAIGALAPLPQSKFRDGLIDLAQFA
                     VQRRY"
     gene            2291..2605
                     /gene="rplU"
                     /locus_tag="VM692_03025"
     CDS             2291..2605
                     /gene="rplU"
                     /locus_tag="VM692_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HUQ51166.1"
                     /translation="MYAVFRTGGKQYRARQGERVRVERLDAAVGDAVAFDQVLLVGEG
                     AQVKVGAPLVNGGKVEAKVVSQGRADKITIIKFRRRKHYRRTQGHRQAFTEVEITAIS
                     GA"
     gene            2624..2881
                     /gene="rpmA"
                     /locus_tag="VM692_03030"
     CDS             2624..2881
                     /gene="rpmA"
                     /locus_tag="VM692_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011045240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HUQ51167.1"
                     /translation="MAHKKAGGSSKNGRDSESKRLGVKRFGGEQVLAGNIIVRQRGTN
                     YHAGANVRMGRDHTLYAATAGRVEFGNKGAKSRRFVSVLAD"
     gene            2961..4013
                     /gene="obgE"
                     /locus_tag="VM692_03035"
     CDS             2961..4013
                     /gene="obgE"
                     /locus_tag="VM692_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HUQ51168.1"
                     /translation="MKFIDEADIRVEAGNGGRGCVSFRREKFVPRGGPDGGDGGDGGS
                     VYLVAREGVNTLADFRVKRRFRAESGGGGSGKDMAGAAGEDLYVEVAKGTEVHDLETG
                     EKLGDLTRDGDTLLVAKGGQGGRGNTRFKTSVNRAPRQFDVGGEGERRQLKLALKLLA
                     DVGLLGSPNAGKSTLTRALSAARPKVADYPFTTLHPQLGVVRVDTERSFVIADIPGLI
                     EGAAQGAGLGIQFLKHLERTRLLLHIVDACAELSGGDVVETYRAVEGEVGQYSEALKG
                     KPRWLVLNKLDLLPEAQRLKRVAALVKKLKWKGPVYGVSALTADGTKRLVQDVMTFLE
                     KKDEPEETAEPSGHRA"
BASE COUNT          700 a         1287 c         1370 g          707 t
ORIGIN      
        1 atcaatgccg cgctgctgag ccgcggaatc tttcgcgagg ctcccgccgg cagcgccgtc
       61 gcgccgacga gcggcgcaaa atggctcgcg gcaacttcgc tcgtgttctg gttcggcgcc
      121 atcgtctgcg gccgtttcat cgcgtacgtc tcatgatgga cttcttcgtt tggctcgaga
      181 gcacgccgat cgcgatcttc atacgcgact atcgctttgc ttacgcaacg atcgaaagcg
      241 ggcattacgt cggtctgtcg ttgctcgtcg gcggcatcat gctgatcgac ctgcgcgtgc
      301 tcggcatggc gcgagtcctg ccgttgaaaa acatgatcgg tctgctgccg atcgtgtgga
      361 tcggcttcat catcaacgtg ctgacgggct cgctactctt catctacggc gcgacaaact
      421 tcggtttcaa tcgagcgttc cacctcaaga tggccttcat ggtcgtcgcc ggcttgaatg
      481 cgtttgcgtt cgacctcgcc gtgcgcaggt ccggcggcga ttgggtcgtg gcagaccggc
      541 cgccgccgat cgtcaaaggt ttcgccaccg cgtcgctcgt gttctggctt tgcgtcgtga
      601 cgacgggccg gtggatggcc tacctctaag ggcgccggcg ttccggtttg ggtcgaaccg
      661 aaccgtaaca ccggcactga aggtgatttt atgagcgaca agccgaccgc cgctgaacag
      721 accgagctcg aggccgccgt gctgcgccgt ttgctgcggc acttcgacga gcacaaagac
      781 gtgcagaaca tcgatttgat gattctcgcg ggcttctgcc gcaactgctt gagcaagtgg
      841 atggtcgagg cggccaacga gcgcggcctc gaagtggggc tcgacgaagc gcgcgagagg
      901 atctacggca tgccgtactc ggactggaag gaacgctttc agccgccggc caccgaggaa
      961 cagctcgcgc ggctcgccgc cgtcgagaaa gccaaaaaga acaagtagcc cagcgcgcga
     1021 gcgctcagta acgtcgctgc accgcgaact gtgcgaggtc gatgagcccg tcgcggaact
     1081 tcgactgcgg cagcggcgcc agggcgccga tcgcgagctc gacctcggca cgggcgcgct
     1141 cggccgtgta gcgcaggccg cccgacgttt cgatcgcgag ctgaatcgaa gtcaaattgc
     1201 gcgtgccgcc ggtctcgacc gcctcgcgga tcatgtgccg ctcggcggcg ctgccgtgcg
     1261 ccatcgcgta gatcagcggc agcgtgggct taccttcggc aaggtcgtcg ccgatgttct
     1321 tgccgagctc ctcgaccttg gcgtcgtaat cgagcgcgtc gtcgacgagc tggaacgcct
     1381 ggcccagatg ccggccgtag gccgcaagcg cttgctcgac ggcgtcgtcc tgcttcgcga
     1441 ggatcgcccc gatccgcgtg ccggcctcga atagcttggc cgtcttgcgg tagatgacct
     1501 ccatgtagtc ggcctcgctg atgtcggggt tgttgcagtt catgagctgc aacacctcgc
     1561 cttcggcgat cgtgttggtc gcgttcgcca gcacgtcgag gatcctgatc tcctcgagct
     1621 cgaccatcat ctggaacgag cgcgagtaga ggaagtcccc gacgagcacg ctcgcctggt
     1681 tgccgaacac ggcattggcg gtctcgcggc cgcgcctgag cccggagctg tcgacgacgt
     1741 cgtcgtgcag cagcgtcgcc gtatggatga actcgatgat cgcggccgcc gtgatatggt
     1801 cctgactcga ccccgcgcca caggcgcgag cggccaatag cacggcgagc ggccgcaggc
     1861 gcttgccgcc gttatgaatg atgtggtgcg cgacctgatt gacgagcgcc acgtcgctgc
     1921 cgagccgccg cacgatcgtc cgattgacgt cattccaatc gtcaccgacg agcgcccgca
     1981 gcgcctcgag cgacggcaga tccggattcg tcttcgcgag cgacatgttg cgattttgcc
     2041 tcatcgcccc cccgcatagc gaatcgcaag cggcatggga agccgagcta ggcgaagcgc
     2101 gctgcagggc ctggcctcga gcggcctcgc caacgtcgaa cgctttgcgg cgcggcacga
     2161 cgtgacgtga cgcaaagccg acgcagccca aacaggccgc ccgtcgcgtt gaccgcgcgc
     2221 aggcaactct ctagaattgc gagcctttcc gagggggttc accctcgaaa tcgcttggga
     2281 gtatgtagtc gtgtatgccg ttttccgtac tggcggtaag caataccgcg cccgtcaagg
     2341 cgagcgcgtt cgcgtagagc gcctcgacgc cgccgtcggc gatgccgtcg ccttcgatca
     2401 ggtgttgctc gtcggcgagg gtgctcaagt caaagtcggc gcgccgctcg tcaacggcgg
     2461 caaggtcgag gcgaaggtcg tctcgcaggg ccgcgcagac aagatcacga tcatcaaatt
     2521 tcgacgccgc aagcactacc gccgcacgca gggccatcgt caggccttta ccgaagtcga
     2581 gatcacggcg atcagcggcg cctaagaagc aaggagtagc gagatggcac acaagaaagc
     2641 aggcggcagc tcgaagaacg gccgcgactc ggaatcaaag cggctgggcg tcaagcgctt
     2701 cggcggcgag caagtgcttg ccggcaacat catcgtgcgc cagcgcggca cgaattatca
     2761 cgccggcgcg aacgtgcgta tgggccgcga ccacacgctg tatgccgcga ctgcgggccg
     2821 cgtggagttc ggcaacaaag gggcgaagag ccggcgtttt gtgagcgtgc tcgcggacta
     2881 aagcgtccgc gctgctcgcg tcgctcacga aggccccgca ctgcggggct ttttcgtttc
     2941 tcgctcgcga gtgctgcact atgaaattca tcgatgaggc cgacattcgc gtcgaagccg
     3001 gcaacggcgg ccgtggctgc gtcagctttc gccgcgagaa gttcgtgccg cgcggcggcc
     3061 ccgacggcgg cgacggcggc gacggcggca gcgtctatct cgtggcgcgc gaaggcgtga
     3121 acacgcttgc ggatttccgc gtcaagcgcc gcttccgcgc cgagagcggc ggtggcggct
     3181 cgggcaagga tatggccggc gcggcgggcg aggacctcta cgtcgaggtc gcgaagggca
     3241 ccgaggtgca cgacctcgag accggcgaga agctcggcga cctcacgcgc gacggcgaca
     3301 cgctgctcgt ggccaaaggc gggcagggtg gccgcggcaa cacgcggttc aagaccagcg
     3361 tcaaccgcgc gccgcgccag ttcgacgtcg gcggcgaggg cgagcggcgg cagttgaagc
     3421 tcgcgctaaa gctgttggcg gacgtcgggc tgctggggtc cccgaacgcc ggcaagtcga
     3481 cgctgacgcg cgcgctgtcc gcggctcggc cgaaggtcgc cgactatccg ttcacgacgc
     3541 tgcatccgca gctcggcgtc gtgcgcgtcg acaccgagcg cagcttcgtc atcgcggaca
     3601 ttccggggct catcgaaggc gccgcgcagg gcgccgggct cggcatccag tttctgaagc
     3661 atctcgagcg tacgcggttg ttgctgcaca tcgtcgatgc ctgcgccgag ctctcgggcg
     3721 gcgacgtcgt cgagacgtat cgcgccgtcg aaggcgaggt tgggcaatat tccgaggccc
     3781 tgaaaggcaa accgcgctgg ctcgtgttga acaagctcga tctgttaccg gaggcgcagc
     3841 gcttgaagcg cgtcgcggcg ctcgtcaaaa aactgaagtg gaaggggccc gtctacggcg
     3901 tcagcgcgct gacggccgac ggcacgaaac gtctcgtgca ggacgtgatg acgttcttgg
     3961 agaagaaaga cgagcccgag gagacggcgg agccgtcagg ccatcgcgcg taggcgagcg
     4021 ttgatctggc tcttgtagtg cgcggcgcga tttttccgca agat
//
LOCUS       DATLEE010000141         2870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3877028, whole genome shotgun sequence.
ACCESSION   DATLEE010000141 DATLEE010000000
VERSION     DATLEE010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3877028"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="secD"
                     /locus_tag="VM692_03040"
     CDS             complement(<1..811)
                     /gene="secD"
                     /locus_tag="VM692_03040"
                     /inference="COORDINATES: protein motif:HMM:NF025098.4"
                     /note="part of the preprotein secretory system; when
                     complexed with proteins SecF and YajC, SecDFyajC
                     stimulates the proton motive force-driven protein
                     translocation, and appears to be required for the release
                     of mature proteins from the extracytoplasmic side of the
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HUQ51169.1"
                     /translation="MNRYPLWKNLLVFGVVIVSTIIALPNVFGDDEAVQLSRSDGVAV
                     DEPTLEQLRTKLMDSQLPYLSAELEGTGAVVRVETPELQQQAQAALSDAFPSPYVVAL
                     TLSPRTPGWLRALGLGPMLLGLDLRGGVHFLYEVDLDTAISQYLETYETDLRAQFRPR
                     NIRNDVRVVGTEMHVAILEPSDLDAAEQIIRQLDTGDQLVQLGQVSGRLDIQRGTVDG
                     RQGFIVRLTPSAIRERQDFAIQQNTVTLRNRVNALGVAEAVVQRQGLDRILV"
     gene            complement(829..1173)
                     /gene="yajC"
                     /locus_tag="VM692_03045"
     CDS             complement(829..1173)
                     /gene="yajC"
                     /locus_tag="VM692_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HUQ51170.1"
                     /translation="MDFLLSFFISDAVAQTAAPAGAPSMVSTLLLPVMLIVVFYFLLI
                     RPQQKKQKEHTAMVGALVVGAEVVTGGGVLGKITEVGDTFLTVEIADGVNVKVQRHSI
                     TAVLPKDTIKHA"
     gene            complement(1213..2355)
                     /gene="tgt"
                     /locus_tag="VM692_03050"
     CDS             complement(1213..2355)
                     /gene="tgt"
                     /locus_tag="VM692_03050"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HUQ51171.1"
                     /translation="MKFELRKQDGGARRGRISFRRGVIETPAFMPVGTYGAVKAMTPE
                     ELEGLGAQIILGNTFHLLLRPGIDVIRAHGGLHAFTHWQRPILTDSGGFQVWSLQSLR
                     KITEEGVEFRSPVNGDLIHLSPERSIEMQDALGADIVMVFDECTAYPADRATARESME
                     LSLRWAKRSRAAFDSLRRGKDSDAALFGIVQGGVHDDLRRESLAGLEAIDFPGYAVGG
                     LAVGEPEAERLAVLEGLTPAMPVDRPRYLMGVGTPADLVKAVARGIDMFDCVIPTRHA
                     RNGQLFTSEGPLNIRNSRFQADTGPIDPNCACYACRNYSRSYLRHLQQCNEILGARLA
                     TIHNLHYYLALMARMRAAIEEGRFAALAAEVASAPAVSTAEPGPVP"
     gene            complement(2352..>2870)
                     /locus_tag="VM692_03055"
     CDS             complement(2352..>2870)
                     /locus_tag="VM692_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HUQ51172.1"
                     /translation="TAGLHFDAAMLDALAARGVEGAFLTLHVGAGTFAPVRTQRIEDH
                     ELHAEWLEVSVDACAAVERCRKRGGRVVAIGTTSVRALETAARGGKLAPFAGDSRLFI
                     YPGFEFRVVDAMVTNFHLPESSLLMLVSAFAGREAVLAAYQHAVTQRYRFFSYGDAMF
                     VTPQAAARPRRA"
BASE COUNT          508 a          940 c          950 g          472 t
ORIGIN      
        1 cgacgaggat gcggtcgagc ccttgccgct ggacgacggc ttccgcgaca ccgagcgcgt
       61 tgacgcgatt gcgcagcgtc acggtgtttt gctgaatggc gaagtcttgc cgctcgcgaa
      121 tcgcggacgg cgtgaggcgc acgatgaacc cctgccgacc gtcgacggtg cctcgctgga
      181 tgtcgagccg acccgagacc tggcccagtt gaacgagctg atcgccggtg tcgagctggc
      241 ggatgatctg ctccgcggca tcgagatcgc tcggctcgag gatcgcgacg tgcatctccg
      301 tgccgacgac gcgaacgtcg ttgcggatgt tccgtgggcg aaattgcgct cggaggtcgg
      361 tctcgtaagt ctctagatac tgcgagatgg ccgtatcgag atcgacctcg tacagaaaat
      421 gcacgccgcc gcgtaggtcc aaaccgagca gcatcggccc caggccgagt gcccttagcc
      481 agcccggcgt ccgtggcgag agcgtcagcg ccacgacata cgggctcgga aaagcgtcgc
      541 tgagcgcggc ttgagcctgt tgctgtagct cgggcgtttc gacacgcacg accgcccccg
      601 tgccctcgag ctcggcggac agatagggaa gctggctgtc catgagcttg gtgcgcagct
      661 gctcgagcgt cggctcgtcg acggcgacgc cgtccgagcg cgacagctgg acggcctcgt
      721 cgtcaccgaa cacgttcggc aaagcgatga tcgtcgacac gatcacgacg ccgaacacca
      781 gcaggttctt ccacagcgga tagcggttca tacgggtaac tcgcgccgtt aggcgtgctt
      841 gatcgtgtcc ttgggcaaca cggcggtgat ggagtggcgt tgcactttga cgttcacgcc
      901 gtcggcgatt tcgacggtga ggaacgtgtc gccgacctcg gtgatcttgc cgagcacgcc
      961 gccgcccgtg acgacttccg cgcccacgac gagcgcgccg accatcgcgg tgtgctcctt
     1021 ttgcttcttc tgctgcggac ggatcagcag gaaatagaac acgacgatga gcatgacggg
     1081 gagcagcagc gtgctgacca tgctcggtgc cccggccggg gcagccgtct gcgcgactgc
     1141 gtcgctaatg aaaaagctga gcaggaaatc catgagccgt gagggtccgt gaggctaaaa
     1201 acgaggcggg cattatggca caggcccagg ttccgccgtc gaaacagctg gcgcgctggc
     1261 gacctccgcg gcgagtgccg cgaagcgccc ctcctcgatg gcggcacgca tgcgcgccat
     1321 cagcgccagg tagtagtgca ggttgtgtat cgtcgcgagc cgcgccccca agatttcgtt
     1381 gcactgctgt aagtggcgca ggtacgaccg cgagtagttg cgacacgcgt aacaggcgca
     1441 attgggatcg atcggcccag tatcggcctg aaagcggctg ttgcggatgt tcagcggccc
     1501 ttcgctcgtg aacagttggc cgttgcgcgc gtgccgagtc gggatcacgc agtcgaacat
     1561 gtcgatgccg cgagcgacgg ctttgacgag atcggccggc gtcccgacgc ccatcagata
     1621 gcgcggccga tcgacgggca tcgcaggcgt aagcccttcg agcacggcga gccgctccgc
     1681 ttccggctcg ccgacggcga gaccgccgac ggcatagccc gggaaatcga ttgcctcgag
     1741 cccggccagc gactcgcggc gcaagtcgtc gtgcacgccg ccttggacga tgccgaacaa
     1801 cgcggcatcg ctgtccttgc cgcgcctcaa cgagtcgaac gcggcgcggc tgcgttttgc
     1861 ccagcgcagc gacagctcca tcgactcgcg cgccgtcgcg cgatccgcgg ggtacgccgt
     1921 gcactcgtcg aacaccatga cgatgtcggc gccgagcgcg tcctgcatct cgatcgagcg
     1981 ctcgggcgag agatgaatga ggtcgccgtt gaccggcgac cggaactcaa cgccctcctc
     2041 cgtgatcttg cgcaagctct gcaggctcca aacctggaag ccgccggagt ccgtaagaat
     2101 cggccgctgc cagtgcgtga acgcgtgcaa cccgccgtgc gcgcgaatga cgtcgatccc
     2161 gggccgcagt agcaaatgaa acgtgttgcc gagaatgatc tgtgcgccga ggccttcgag
     2221 ctcctctggt gtcatcgctt tcacggcgcc gtaagtgccg acgggcatga acgcaggcgt
     2281 ctcgatgacg ccgcggcgga acgaaatgcg cccgcgccgc gcgccgccgt cttgcttccg
     2341 cagctcgaac ttcatgcgcg ccttgggcga gccgccgcct gcggcgtgac gaacattgcg
     2401 tcgccgtagc tgaagaagcg ataccgctgc gtgaccgcgt gctggtaggc cgcgagcacc
     2461 gcctcgcgtc cggcaaatgc cgagaccagc atcagcaatg acgactccgg caggtggaag
     2521 ttggtcacca tcgcatcgac cacgcgaaac tcgaaacccg gatagatgaa gagccggctg
     2581 tcgcccgcga acggtgccag cttgccgccg cgtgcggcgg tttccaacgc gcgcacgctc
     2641 gtcgtgccga tcgcgacgac gcgaccaccg cgcttgcggc agcgctcgac agcggcgcac
     2701 gcgtctacgg acacttccaa ccactcggca tgcagctcgt gatcctcgat gcgctgcgtg
     2761 cgcacgggcg cgaacgtacc ggcgccgacg tgcaacgtca aaaacgcgcc ctcgactccg
     2821 cgtgctgcga gcgcgtcgag cattgcggcg tcgaaatgca gccctgcggt
//
LOCUS       DATLEE010000142         2214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1642376, whole genome shotgun sequence.
ACCESSION   DATLEE010000142 DATLEE010000000
VERSION     DATLEE010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642376"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VM692_03060"
     CDS             complement(<1..641)
                     /locus_tag="VM692_03060"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ51173.1"
                     /translation="MPHRLSILAAVALALPAADAVAAHRDDDGIVVQDLAYGEVLFEF
                     FQEDYFSALTRLLAAQQRGELEHHGAEAELMLGGLYLSYGEHRLAGEIFERVLQQSVE
                     PALHDRAWFFLAKIWQQRGYLNEAEAALARITGELPEELEPERQMLYAQALMEQGRFA
                     DALAALEAWRRPGDAWVGYAKYNIGVSLVRLGQVEAGARVLDEVGQLDPENPA"
     gene            complement(645..1226)
                     /locus_tag="VM692_03065"
     CDS             complement(645..1226)
                     /locus_tag="VM692_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007018940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HUQ51174.1"
                     /translation="MVVKWIASVSLGVLAAQPFGVGAQEVAAVPEFAPRADQGAEQRT
                     EFRTLDEDVQDLKKQVLDLNRDLFLLEEELLFPSNTQTAVFISMDVGEFFGLDSVELK
                     IDDKDVANYLYTEREAGALLKGGVQRLYIGNLKAGEHELVAVFTGEGPHARDYRRAAS
                     LVFEKGIGPKYIELTISDREVKQQPEFLVKEWE"
     gene            complement(1255..1761)
                     /locus_tag="VM692_03070"
     CDS             complement(1255..1761)
                     /locus_tag="VM692_03070"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HUQ51175.1"
                     /translation="MKGRIRARWSGAALASLLAVAGSVSAATQLLGSEAPDFVLKSLA
                     GKNLRLSEYRGEVVMLSFWATWCGDCRAQLVELGEMRERYQDAGVELLAVSLDQNARQ
                     AGAMTAEASYPVLHDPAGEVGRLYDVTKMPVMVLIDRGGVVREVFEGFKRGNEEQYLE
                     RVQALLRE"
BASE COUNT          390 a          769 c          681 g          374 t
ORIGIN      
        1 aacgccggat tctcggggtc gagctggccc acttcgtcga gcacgcgcgc gcccgcctcg
       61 acttgaccga ggcgcaccag cgacacgccg atgttgtact tcgcgtagcc gacccacgca
      121 tcgccggggc ggcgccaagc ttcgagcgcc gctagagcat ccgcgaaccg gccctgctcc
      181 atgagcgcct gcgcgtagag catttgccgc tcgggctcga gctcctcggg caactcgccc
      241 gtgatgcgag ccagcgccgc ttcggcttcg ttcaggtagc cgcgctgttg ccagatcttg
      301 gcaaggaaga accacgcgcg atcgtgcagc gcgggctcga ccgattgctg caagacgcgc
      361 tcgaagattt cgcctgcgag ccgatgctcg ccgtacgaca gatacaagcc gccgagcatg
      421 agctcggctt cggcgccgtg atgctcgagc tcaccgcgtt gctgcgcggc gagcaagcgc
      481 gtcagcgccg aaaaatagtc ctcttgaaag aactcgaaca agacctcgcc gtaagcgagg
      541 tcctggacga cgatgccatc gtcgtcgcgg tgcgcagcaa ccgcgtcggc ggccggcagc
      601 gcgagcgcga ccgccgccaa gatggagaga cggtgaggca ccggctactc ccactccttg
      661 acgaggaact ccggctgttg cttgacctcg cgatccgaga tcgtgagctc gatgtacttg
      721 ggacctatgc ccttctcgaa gaccagcgac gcggcgcggc gatagtcgcg cgcgtgcggc
      781 ccttcgcccg tgaacacggc gacgagctcg tgctcgccgg ctttcaggtt gccgatgtag
      841 agccgctgca cgccgccctt cagcaacgca ccggcctcgc gctccgtata gagatagttc
      901 gcgacgtcct tgtcgtcgat cttgagctcg acggaatcga ggccgaagaa ctcgccgacg
      961 tccatcgaga tgaataccgc ggtttgcgtg ttcgacggga acagcagctc ctcttcgagc
     1021 aggaacaggt cgcgattcag atcgagcacc tgcttcttga gatcctgcac gtcctcgtcg
     1081 agcgtgcgga actcggtccg ctgctcggcg ccttgatcgg cacgcggtgc aaactccggc
     1141 accgccgcaa cttcctgcgc gccgacgccg aacggctgcg ccgcgagcac accgagcgac
     1201 accgacgcga tccacttgac taccatcgta agctccaacc gccgtttcga accgtcattc
     1261 acgcagcaac gcttgcacgc gctcgaggta ctgctcctcg ttgccgcgct tgaaaccttc
     1321 gaagacctcg cgcacgacgc cgcctctatc gatcagcacc atcacgggca tcttcgtcac
     1381 gtcgtagagc cgcccgacct cgcccgcggg atcgtgcaac accgggtagc tcgcctcggc
     1441 cgtcatcgcg ccggcttggc gcgcgttctg gtcgagactc acggccagca actcgacgcc
     1501 ggcatcctga tatcgctcgc gcatttcgcc gagctcaacg agctgcgcgc ggcaatcgcc
     1561 gcaccacgtc gcccagaagc tcagcatgac gacctcgcct cgatactcgg acaggcgcag
     1621 gttcttgcct gcgagactct tcagcacgaa gtcgggcgct tcggagccga gcaactgcgt
     1681 tgcagcggaa acgctgcccg caacggcaag caagcttgcg agcgctgcac cgctccatcg
     1741 cgctctgatg cgtcctttca tgccacggtc ctcgccacgg tcgtccacgc ggagtcgttc
     1801 gaagcggcgc cggaaaccct agtccggcgc ggcctcgagg gctatgagcc aggtctcaat
     1861 cgctttctcg ggtgttttcc gcaggggtga gcacgaaacg tgccactagt cacaaacgcg
     1921 ttgtaagccg cgccgctgct gacttttcgt cagtagcgcc gcgaacgcag tggacgcgat
     1981 ttgtcggcgc gcggctacag ggccggtgct tgaagtacag gaaacactgc gaaaacggct
     2041 cgttgcgcgt gtcgccgcgc accgacacgg gtgacgcccc ttggtagtcc ccgaaggggg
     2101 acgccccttg gtagtccccg aagcggcgtc aagggcgctt agccgccgac ccaggcgcgc
     2161 gcgttgtcga acaaccgctg ccacggcgag cattcgcccc aggcggccgg gtgc
//
LOCUS       DATLEE010000143         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3878977, whole genome shotgun sequence.
ACCESSION   DATLEE010000143 DATLEE010000000
VERSION     DATLEE010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3878977"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VM692_03075"
     CDS             <1..626
                     /locus_tag="VM692_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUQ51176.1"
                     /translation="RYIDSPTESLPELYFSYRQRPANLATSTTNVTLLVRTDSNPGRL
                     AALFRSVVREADPNLVTEAVMMLDDQLVATNLALPRLYALLLGGFATLALLVAGVGLF
                     GVLSYVVAQRTRELGIRAALGARPQDLLRLVLRQGLGTTIVGLAVGLVAAGVLMRFVT
                     TLLYGVSSADLPTYVGVPALLLVVAAAACIVPARRAARLDPLRALRQ"
     gene            687..1589
                     /locus_tag="VM692_03080"
     CDS             687..1589
                     /locus_tag="VM692_03080"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HUQ51177.1"
                     /translation="MIDAVERPAVCASCGRPLEGRYCSSCGEQALDPGELTVWHFLTR
                     TVVHELFNFDGKIWRTLGLLLFHPGFLALEYATGRRRPYVNPLRILLVTIIAYVLATQ
                     AGTGFTLNVGPLKLGMAPAPASPTRSIAATLEQVDRFGILGDMFEQRFGSFESASTEI
                     RDRFNRALNGFATPLSFTTVLFVALTLYVLLHRRRPLLVEHAVFSMHYYSFVLSSLLL
                     PLFTVRFGLTRGYAGVAVILLVNLWQLAYLAIAIRRFYFPAGSSRLLAWTASTTIAPV
                     VFLLNPLYLTVIQFVGGAYAIWRL"
     gene            complement(1584..>2047)
                     /locus_tag="VM692_03085"
     CDS             complement(1584..>2047)
                     /locus_tag="VM692_03085"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HUQ51178.1"
                     /translation="NTINTVSYDHPDPSIFTVLTSPTAVAGTANVDFGLIADRWSVAR
                     NTFRPPPFHRNVCSEFVGLVQGRYIGKAEGFEPGCASLHNCMSGHGPDNDAFEAGSRA
                     ADAPQYFEDTLTIIFETQLVIKPTRFAVETELLERDYYRHWQGLKKNFRRL"
BASE COUNT          302 a          654 c          658 g          433 t
ORIGIN      
        1 tgcgctacat cgacagcccc acggagtcgt tgccggagct ctatttctcc taccggcaga
       61 ggccggccaa tctcgcgacc tcgaccacga acgtaacgct gctcgtgcgc accgactcga
      121 acccggggcg gctcgcggca ctgtttcgaa gcgtcgtacg cgaggccgac cccaatctgg
      181 ttaccgaagc ggtcatgatg ctcgacgatc agctcgtggc gacgaacctc gcgttgccgc
      241 gcctctatgc gctgctgctc ggcgggttcg cgacgctcgc gttgctggtc gcgggagtcg
      301 ggcttttcgg cgtactgtcc tacgtcgtcg cgcagcgaac gcgcgagctc ggcattcgcg
      361 cggcgctcgg ggcgcgtccg caggatctcc ttcggttggt gttgcggcag gggcttggaa
      421 cgacgatcgt gggcctcgcc gtcgggctcg tggcggcagg cgtgctcatg cgtttcgtga
      481 cgacactgct ctatggcgtt agctccgccg accttccgac gtacgtaggc gttccggccc
      541 tcctgctcgt cgtcgccgca gcggcctgca tcgtcccggc ccgacgcgcc gcgcggctcg
      601 atcccttgcg agcgctgcgc caatgagtcg agttaccggc ggaaattcgt ctcgagccgg
      661 aacccatccg cgatgacgtc gcaacgatga tcgatgcggt cgagcggcct gcggtttgcg
      721 cgagctgcgg ccggccgctc gaggggcgct actgttcgag ctgcggagag caggccctcg
      781 atcccggcga gctcacggtc tggcacttcc tgacgcgcac ggtcgtacac gagcttttca
      841 atttcgacgg caagatttgg cggacgctcg gtctgttgct gtttcacccg gggttcctcg
      901 cgctcgagta cgcgaccggg cgccgtcgtc cgtacgtcaa cccgctgcgg attctgctcg
      961 tgacgatcat cgcgtacgtg ctggcgacac aggcgggtac cggctttacg ctcaatgtcg
     1021 gcccgctgaa gctcggtatg gcgcccgccc cggcgtcccc gacgcggtcg atcgcagcca
     1081 cgcttgagca agtggatcgt ttcgggatcc taggggacat gttcgagcag cgattcggct
     1141 ccttcgagag cgcgtcgaca gagatccgcg accgattcaa tcgagcgctg aacggcttcg
     1201 cgacgccgct gtcattcacg accgtcttgt tcgttgcgct gaccctctat gtcttgctcc
     1261 accggcggcg gccgctgcta gtcgagcatg cagtattcag catgcattac tacagcttcg
     1321 tgctgtcatc gctgctattg cctctgttca cagtgaggtt cggtctcacg agaggctacg
     1381 ccggcgtggc ggtgattctg ctcgtcaatc tttggcagct cgcgtatctc gcgatcgcga
     1441 tccgacggtt ttattttcct gccggttcaa gccgacttct cgcttggacc gcgtccacga
     1501 ctatcgcgcc cgtcgttttc ctgctgaatc cgctgtacct cacggtcatt cagttcgtgg
     1561 gcggggcgta tgccatctgg cggctatagt cgccggaaat tcttcttcag cccttgccag
     1621 tgacgatagt agtcgcgctc gagtagctcg gtttcgacgg cgaagcgcgt cggcttgatg
     1681 acgagctgcg tctcgaaaat gatcgtcaga gtgtcctcga aatactgcgg cgcgtcggcg
     1741 gcgcgcgagc cggcttcgaa cgcgtcgttg tcggggccgt ggcccgacat gcagttgtgc
     1801 aagcttgcgc aaccgggctc gaagccttcc gccttgccga tgtagcggcc ttgcaccagg
     1861 cccacgaact cgctgcaaac gttgcgatgg aagggcggcg gccgaaacgt gttgcgcgcc
     1921 acggaccagc ggtcggcgat caagccgaag tcgacgttcg ctgtgccagc aaccgcggtc
     1981 ggcgatgtca gcaccgtgaa gatcgacggg tccggatggt cgtagctgac ggtgttgatc
     2041 gtgttga
//
LOCUS       DATLEE010000144         4407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6038918, whole genome shotgun sequence.
ACCESSION   DATLEE010000144 DATLEE010000000
VERSION     DATLEE010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6038918"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VM692_03090"
     CDS             complement(<1..192)
                     /locus_tag="VM692_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrrolo-quinoline quinone"
                     /protein_id="HUQ51179.1"
                     /translation="MAPLANAQIRGDFVPVTDAMLQNPAPADWLMWRRTLDLWGYSPL
                     TQIDRGNVGELRMVWTRALG"
     gene            complement(255..1034)
                     /locus_tag="VM692_03095"
     CDS             complement(255..1034)
                     /locus_tag="VM692_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51180.1"
                     /translation="MRASSLATLICCALAAPLALAQQAPATPPASARAAAPVDFTGTW
                     VSPVMEDWRWRMVTPLKGDAASIPVNAAARAVIDAWDPAKDEAAGDLCKAYGAPALLR
                     VPGRLRIRWLDDDTLEIQADAGRQTRVLHFGAAPPAGIEPSRQGYSQAGWSFLTARPG
                     PGGVPLGMAPGRPAPSRTLEVTTTHLLPGYLRKNGVPYSGETSVLEYFDLFTEPDGAQ
                     WFTVTTIVTDPVYLGLPFVTTTDFRKERDGANFNPRPCMAR"
     gene            complement(1031..1966)
                     /locus_tag="VM692_03100"
     CDS             complement(1031..1966)
                     /locus_tag="VM692_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ51181.1"
                     /translation="MTRATVVAFAAAALATAVGEAQQDFSAVQLSVLPVQGGVYMIAG
                     AGGNISVQVGDEGVLLVDTEFAPLAPKIMAEIRKLSSGPVRFIINTHDHPDHVGGNAA
                     LAELIQPTSLEPLQMIAHENALDRLSNPPAGTAPTPPGGLPADSYFTPTKDFHFNGEA
                     VFLYHAPNAHTDGDTIVLFRESDVVSTGDLFTPGGYPFIDRARGGSVQGLIAGLNQVL
                     HLTVPAKTQERGTYVIPGHGRICDEADVVEFRDMIVIVRDRIADMINRGMTLKQIQAA
                     RPTLDYDTEYVREGSFITAAAFVEAVHSSLTEQAP"
     gene            complement(1963..2874)
                     /locus_tag="VM692_03105"
     CDS             complement(1963..2874)
                     /locus_tag="VM692_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51182.1"
                     /translation="MRNGHGCALAFCLALAGASAHAQVDFSGNWVPLFHEDQPERLPG
                     PELGDYAGIPLNEAGRLRGDSYDADRISVVTEYQCRPHGGDYSMRGLANMRVEQILDS
                     VTQRVLAIHLRMNFQEQERTIWLDGREHPAPLAAHTFAGFSTGSWNGNMLDAATTHLK
                     ENYLRRNGLPRSADATFTEHWVRHGDYLTITTVIRDPAFLTEPLVRSQTWIIAPAQRL
                     GRDVCSYVPELVGEPDYVPNHLPGTNPFLREVIDWYGLPEAGVRGGSETLYPEFLEKL
                     GVPTAAPPRQCQRYCRCGEFGRGCDLR"
     gene            complement(2879..3859)
                     /locus_tag="VM692_03110"
     CDS             complement(2879..3859)
                     /locus_tag="VM692_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ51183.1"
                     /translation="MKLRNRVSFPSILAPALLAALTFTKLHAESINPPTAVQTSPAMT
                     QQERQNLALVLDWWREVIQGGHMELSAKYQADDYIQHNPNVPTGRAAFVTFFQNVVGV
                     KPKNPIPTTLSPGPVVSAAKGDFVFLVFEQESPDPRDATKTYHHNSFEVLRMANGKVQ
                     EHWDSAKRMPLPAGAPKPPAFEQPASQMARGNMGTLSADERKNVELGVLELKDMLQYG
                     HLELADKVMDPGYIQHNPNVPQGRDGFKQFMSRTPGRTPQDIKPEWVRPPSLTLASGP
                     YVLMMWDRTEKDPNDATREYVWNHFDVIRIENGLIKEHWDEAVIAAPAAR"
     gene            4054..>4407
                     /locus_tag="VM692_03115"
     CDS             4054..>4407
                     /locus_tag="VM692_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ51184.1"
                     /translation="MAQHGTTTTTSWDTSYEWKTVALLGVGFGLVGLDRWIIASLFPA
                     MAMDLGLPEGEIGRLAGILGILWGVFAIFSGRLSDSIGHRKVLIPAIILFSLMSGFSG
                     MVQSLVALVWIRATMG"
BASE COUNT          698 a         1423 c         1511 g          775 t
ORIGIN      
        1 gccgagcgcg cgcgtccaca ccatgcggag ctcgccgacg ttgccgcggt cgatctgcgt
       61 cagcgggcta tagccccaga ggtcgagcgt gcggcgccac atgagccaat ccgcgggagc
      121 gggattctgc agcatcgcgt ccgtgactgg gacgaagtcg ccgcgaatct gtgcgttggc
      181 caggggcgcc atgagcacca aaacgagcaa agcggatcta gaggtcgaca cgagcaaagc
      241 cctcgaaaaa gatgtcagcg cgccatgcag ggtcgcggat tgaaattcgc gccgtcgcgc
      301 tccttgcgaa aatccgtcgt cgtcacgaac ggcaagccga ggtagacagg atccgtcacg
      361 atcgtcgtga cggtaaacca ctgcgcgccg tcgggctccg tgaacaggtc gaagtactcg
      421 aggacgctcg tctcgccgct atacgggacg ccgttcttgc gtagatagcc cggcagcaag
      481 tgcgtcgtcg tgacctcgag cgtgcggctc ggcgcgggac gccccggcgc catgccgagc
      541 ggcacgccgc cggggccggg acgcgccgtc aggaagctcc aaccggcctg cgagtagcct
      601 tggcggctcg gctcgatgcc tgccggcggc gcggcgccga agtgcagcac gcgggtttgc
      661 cggccggcgt cggcctggat ctcgagcgta tcgtcgtcga gccagcgaat gcgcaggcgg
      721 cccgggactc gcagcagagc gggcgcgccg tacgctttgc agaggtcgcc ggcggcttcg
      781 tctttcgccg gatcccaggc atcgatgacg gcgcgcgcgg ccgcgttcac gggaatgctg
      841 gccgcgtcgc ctttgagcgg cgtgaccatg cgccagcgcc agtcttccat gacgggcgat
      901 acccaggtgc ccgtgaagtc gacgggtgcc gcggcgcggg ccgacgcagg cggcgtcgcc
      961 ggcgcttgct gcgcgagcgc gagcggcgca gcgagcgcgc agcaaatcag cgtcgcgaga
     1021 gacgacgctc tcatggcgct tgctccgtca agctcgagtg aacggcttcg acgaacgcgg
     1081 ccgccgtgat gaagctgcct tcgcgaacat actcggtgtc gtagtcgagc gtcgggcgtg
     1141 cggcttgaat ctgcttcagc gtcatgccgc ggttgatcat gtcggcgatg cggtcgcgca
     1201 cgatcacgat catgtcgcgg aactcgacga cgtcggcctc gtcgcagatg cggccgtggc
     1261 cggggatgac ataggtgcca cgctcctgcg ttttggccgg taccgtgagg tgcaggacct
     1321 gattcagtcc cgcgatcaat ccttgcacgc tgccgccgcg cgcgcggtcg atgaagggat
     1381 aaccgcccgg cgtgaacaag tcgcccgtgc tgacgacgtc ggactcgcgg aacaacacga
     1441 tcgtgtcgcc gtcggtatgc gcgttcggcg cgtggtagag gaacacggct tcgccgttga
     1501 aatgaaaatc cttcgtcggc gtgaaatacg aatcggcggg caagccgccc ggcggcgtag
     1561 gcgccgtgcc ggccggcgga ttcgagagcc gatccagcgc gttctcgtgc gcgatcatct
     1621 gcagcggctc gagcgacgtc ggttggatga gctccgcgag cgccgcgttg ccgccgacgt
     1681 gatccgggtg atcgtgcgtg ttgatgatga agcgcacggg gccgctcgac agcttgcgga
     1741 tctccgccat gatcttcggt gccagcggcg caaactccgt gtcgacgagc agcacgccct
     1801 cgtcgccgac ttgcacgctg atgttgccgc cggcgccggc gatcatgtag acgccgcctt
     1861 gcacgggcag cacgctgagt tgcacagcgg agaagtcctg ctgcgcctcg ccgaccgctg
     1921 tcgcgagcgc ggcggccgcg aacgccacta ccgtggcgcg cgtcatcgga gatcgcaccc
     1981 gcggccgaac tcgccgcagc ggcaatagcg ctggcactgc cgcggcggcg ccgccgtcgg
     2041 cacgccgagc ttctcgagga attccgggta cagcgtctcg ctgccgccgc gcacgccggc
     2101 ttcgggcaag ccgtaccaat cgatgacctc gcgcaagaac gggttcgtgc ctggcaagtg
     2161 attcggcacg tagtcgggct cgccgacgag ctcggggacg taggagcaga cgtcgcggcc
     2221 aagccgctgc gccggcgcaa tgatccacgt ttggctgcgc acgagcggct ccgtgagaaa
     2281 cgccggatcg cgaatcaccg tcgtgatcgt cagatagtcg ccgtgccgca cccagtgctc
     2341 cgtgaacgtc gcgtcggcgc tgcgcggcag gccgttgcgg cgcaggtagt tctccttcag
     2401 gtgcgtcgtg gccgcgtcga gcatgttgcc gttccagcta cccgtcgaaa aacccgcgaa
     2461 cgtgtgcgcg gcgagcggtg ccgggtgctc gcggccgtcg agccagatcg tgcgttcttg
     2521 ctcctggaaa ttcatgcgca gatgaattgc gagcacacgc tgcgtcacgg agtcgaggat
     2581 ttgctcgacg cgcatgttgg ccaagccgcg catcgagtag tcgccgccgt gcggccggca
     2641 ttggtactcc gtcacgacgg agatgcggtc ggcgtcgtac gagtcgccgc gcaagcggcc
     2701 ggcctcattc agcggaatgc cggcgtagtc gccgagctcg gggcccggta gccgctctgg
     2761 ctggtcctcg tggaacaacg gcacccagtt gcccgaaaag tcgacttgtg cgtgcgcgct
     2821 cgcgccggcg agcgcaaggc aaaacgcgag cgcgcagccg tgaccgttcc gcatcgtgct
     2881 agcgcgcggc cggtgctgcg atgacggcct cgtcccagtg ctccttgatg aggccgttct
     2941 cgatgcgaat gacgtcgaag tgattccaga cgtactcgcg tgtcgcgtcg ttcgggtcct
     3001 tttccgtccg gtcccacatc atgagcacgt acgggccgct cgcgagcgtc agcgacggcg
     3061 gccgaaccca ctcgggcttg atgtcttgcg gcgttcgtcc cggcgtccga ctcatgaact
     3121 gcttgaagcc gtcgcggccc tgcgggacgt tcggattgtg ctggatgtag cccgggtcca
     3181 tgaccttgtc tgcgagctcg agatggccgt actgcagcat gtccttgagc tcgagcacgc
     3241 cgagctcgac gttcttgcgc tcgtcggccg acaaggtgcc catgttgccg cgcgccattt
     3301 ggctcgcggg ctgctcgaac gccggtggtt tcggcgcgcc ggccgggagc ggcatgcgct
     3361 tcgcgctgtc ccagtgctcc tggactttgc cgttcgccat acgcagcact tcgaacgagt
     3421 tgtgatgata ggttttcgtc gcgtcacgcg ggtccggcga ctcttgctcg aacacgagga
     3481 atacgaagtc gcctttcgca gcgctgacga ccgggcccgg agacagcgtc gtcgggatcg
     3541 gattcttcgg cttcacgccg acgacgttct ggaagaacgt cacgaacgcg gcgcgtcccg
     3601 tcggcacgtt cgggttgtgc tggatgtagt cgtcggcctg atatttcgcc gacagctcca
     3661 tgtggccgcc ttggatgacc tcgcgccacc aatcgagcac gagggcgagg ttctggcgtt
     3721 cctgttgcgt catcgccggg ctcgtttgca cggccgtcgg cggattgatc gactcggcgt
     3781 gcaacttcgt gaatgtcagc gccgcgagca gcgcgggggc caaaatggac gggaatgaga
     3841 cgcggtttcg caacttcatg gcaatgcctc cggaacaaac gcggggagtt actcatagta
     3901 cgaacgggcg cacgcggtga cgacacctcg gccgcatcga tgtcgtcgcc ggacgccggg
     3961 ctgcgtatta gcacgggcgc cgacaggccc acagtggtca cggcgccaag agccactaca
     4021 atccggctaa cgcccaaacg aggtttgcga cccatggcac aacacggcac cacgaccacg
     4081 acgagctggg acacctccta cgagtggaag accgtcgcgc tgctcggcgt cggcttcggc
     4141 ctcgtggggc tcgaccgttg gatcatagcc tcgctgttcc ccgcgatggc catggacttg
     4201 ggattgcccg agggcgagat cggccggctc gccggcatcc taggcattct ttggggcgtg
     4261 ttcgcgatct tctcgggtcg gctttcggac tcgatcggtc accgcaaggt cttgattccg
     4321 gccatcatcc tgttctcgct gatgtccggc ttctccggca tggtgcagag cctcgtcgcg
     4381 ctcgtctgga tccgcgcgac gatgggc
//
LOCUS       DATLEE010000145         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_154832, whole genome shotgun sequence.
ACCESSION   DATLEE010000145 DATLEE010000000
VERSION     DATLEE010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154832"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VM692_03120"
     CDS             complement(<1..282)
                     /locus_tag="VM692_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUQ51185.1"
                     /translation="MPQKVPDELALFPPAAAAAEAAPAPLAHRLRPESFDDFLGHEKL
                     LGPGKPLREAIEQGAISSMVLWGPPGCGKTTLARLLARYTDKEFVTFSAV"
     gene            362..>2084
                     /locus_tag="VM692_03125"
     CDS             362..>2084
                     /locus_tag="VM692_03125"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HUQ51186.1"
                     /translation="MVTMVTPGIVRRWTAVAAAAALLVTTVAGAQEERDTRKPALHGK
                     HWVAITGKPLAATAGALTFARGGNAVDAACAMLAAAATMWDTLSWGGETQALIFDPRA
                     GRVRAINALGVAPTGATPEFFKSRGMRGPPEYGPLAAVTPGTPGGLLTMLADFGTLSL
                     AEVLEPALQMAAGYPMEQSQTVNIEASKELLMRWEASRRVLLPNLDPQRPQAWSAPLP
                     GEIFEQPELLATLTKLVDAEREALKAGKNRKQAIMAAYERFYRGDIARELVASTQAAG
                     GLFTMEDLDQWKVYVEDPAVTNYKGIDVYKLNHWVQGPVMLQTLPLLENTDLRSMGYN
                     SARYVHTLYQAMNLAFADRDFYYGDPYVPPVEPIKGLLSKDYARERFGQINWQQNDPH
                     VRPGDPYPFQGATNPYREQLEHWNPRVTEPHRRADNVDEPQMTHDQAFLAGTTSIQAA
                     DAEGWVVSITPSGGWVPAFIAGTTGVGLSQRMQSFVLDPAINPYNVLAPGKRPRATLS
                     PSMALRDGKPFLSFAVQGGDAQDQNLLQFFLNMVEFKMSVQQAVEAANINSYQMQNSF
                     GDHTSEPG"
BASE COUNT          359 a          706 c          694 g          325 t
ORIGIN      
        1 cacggcgctg aacgtcacga attccttgtc ggtgtagcgc gcgagcaagc gtgccagcgt
       61 cgtcttgccg cagcccggcg ggccccacag gaccatgctc gagatcgcgc cttgctcgat
      121 cgcctcgcgc agcggcttgc cgggcccgag cagcttctcg tgacccagaa aatcgtcgaa
      181 cgactcgggg cgcagccgat gggcgagggg cgccggcgcg gcctcggccg cagcggcggc
      241 gggcggaaat aatgcgagct cgtcggggac tttttgcggc attccgagcc cattctactg
      301 gttgcactcg agcgtgacgc ttgccccaag cgtggtcccg agcaggtgtt acactcgacc
      361 catggtcacg atggtcaccc cgggcatcgt tcgccgctgg acggccgtcg cggcggccgc
      421 ggcgctgctc gtcacaacgg tggcgggcgc ccaagaggag cgcgacacgc gcaagcccgc
      481 gctgcacggc aagcactggg tcgcgatcac cggtaaaccg ctcgcggcga cggccggggc
      541 cctgacgttc gcgcgtggcg gcaatgccgt cgatgcggcc tgcgcgatgc tggccgcggc
      601 cgcgacgatg tgggacacgc tgtcgtgggg cggcgagacg caagcgctga tcttcgaccc
      661 gcgcgccggc agagtccgtg cgatcaacgc gctcggcgtc gcgccgacgg gtgccacgcc
      721 ggagttcttc aagtcgcgcg gcatgcgcgg cccacccgag tacgggccgc tggccgccgt
      781 gacgccgggc acgccgggcg gcttgctcac gatgctcgcg gacttcggca cgctgagtct
      841 cgccgaggtg ctcgagcctg ccttgcaaat ggcggccggc tatccgatgg agcagtcgca
      901 gaccgtcaac atcgaggcca gcaaagagct gctgatgcgc tgggaagcgt cgcggcgcgt
      961 cctgctgccg aacctcgatc cgcagcggcc gcaagcgtgg tcggcgccgt tgcccggcga
     1021 gatcttcgag cagcccgagc tgctcgcgac gttgacgaag ctcgtcgacg ccgagcgcga
     1081 ggcgctgaag gccggcaaga atcgcaagca ggccatcatg gccgcctacg agcgcttcta
     1141 tcgcggcgac atcgcgcgcg agctcgtcgc ctcgacgcaa gccgccggcg gtctcttcac
     1201 gatggaagac ctggatcagt ggaaggtcta cgtcgaggac ccggctgtca ccaattacaa
     1261 aggcatcgac gtctacaagc tgaatcactg ggtgcaaggc cccgtgatgc tgcagacatt
     1321 gcccctcctc gagaacaccg acctgcgctc gatgggctac aacagtgcgc gctatgtgca
     1381 cacgctgtat caagcaatga acttggcgtt cgccgatcgc gacttctact acggcgatcc
     1441 ctacgtgccg cccgtcgagc ctatcaaggg cctgctgtcg aaagactacg cgcgcgagcg
     1501 cttcggccag atcaactggc agcagaacga cccgcacgtt cgccccggcg atccataccc
     1561 gttccaaggc gcgaccaatc cttaccgcga gcagctcgag cattggaacc cgcgcgtcac
     1621 ggagccgcat cgtcgcgccg acaacgtcga cgagccgcag atgactcacg accaagcgtt
     1681 cctggccggc acgacgtcga ttcaagcggc cgatgccgag ggttgggtcg tctcgatcac
     1741 gccgagtggc ggctgggtgc ccgcgttcat cgccggtacg acgggcgttg ggttgtcgca
     1801 gcgcatgcag agcttcgtgc tggaccccgc gatcaatcct tacaacgtgc tcgcgcccgg
     1861 caagcggccg cgcgcgacgc tctccccgag tatggcgctg cgcgacggca agccgttcct
     1921 gtcgtttgcg gtgcagggcg gcgacgcgca agaccagaac ttgctgcaat tcttcttgaa
     1981 catggtcgag ttcaagatga gcgtgcagca ggccgtcgaa gccgcgaaca tcaacagcta
     2041 ccagatgcag aactcgttcg gcgaccacac gtcggagccg ggcc
//
LOCUS       DATLEE010000146         2871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3060001, whole genome shotgun sequence.
ACCESSION   DATLEE010000146 DATLEE010000000
VERSION     DATLEE010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060001"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VM692_03130"
     CDS             <1..181
                     /locus_tag="VM692_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51187.1"
                     /translation="DIDGFDTSCFSGEYVTGDVSRSYLDALEVIRSNSAKARRDAKVR
                     AEESEDDTMQVASGL"
     gene            192..1400
                     /locus_tag="VM692_03135"
     CDS             192..1400
                     /locus_tag="VM692_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUQ51188.1"
                     /translation="MRFLIIDDDAEYRQLLRYHLEVEWPDAVIDAQQPSSAGPIADSL
                     DLADVDLVLLGHPMAAHEGLSFLRLLRQRDDCPPILLFAAQSDEFLAVDALKSGAANF
                     FPKDRVKHQRLIEVVRGELRVERLDPTGELLVDHKALNGARKRRLVAKLYAGDLSSVY
                     LAESEEGGERIALKVLRHVPDVGSGRLFDRFLQEYEIIARVHHANVVRIFDLGVADDH
                     AYIAMEYLAAGSLAERLKRALEPTTTADYIRQIAGALAAIHGAGILHRDLKPANIMFR
                     DDGSLALIDFGLAKQMRLHAAITGTGQIFGTPYYMSPEQGHAEPTDERSDLYSLGCIF
                     YEMLTGERPFTASSPMGVIYRHAHAPRPRLSRALTHYQPLLDRLLAVDRYERYQSAGE
                     LLAALEAQLR"
     gene            complement(1412..2131)
                     /locus_tag="VM692_03140"
     CDS             complement(1412..2131)
                     /locus_tag="VM692_03140"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HUQ51189.1"
                     /translation="MTVLFISDLHLTAGDTETTRRFVDFMDGPARAATELYILGDLFE
                     AWIGDDDDDPRLAPIVAAVTRLTAAGIPCGLMHGNRDFLLGQRFCATTGCRLLGDYER
                     VTLFGESVLLTHGDLLCTDDTRYLTLRSELRSPIWQREFLAKSLAERRQIASDLRQLS
                     ATEIAAKSAYIMDVNQQAVERTMREFDVTLLLHGHTHRPAVHRFGLDGKPAARIVLGA
                     WYEQASVVRWTADGFALETIG"
     gene            complement(2137..2718)
                     /locus_tag="VM692_03145"
     CDS             complement(2137..2718)
                     /locus_tag="VM692_03145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUQ51190.1"
                     /translation="MRGKCLLLCLLLTLQVAGAQDASTPASTVAPRVRVVTNAGNFVI
                     ELDKGRSPLTVEAFLKYVNQGFYSGVIFHRVVAGFIAQAGGFTADMKQKDVTSNIFNE
                     SGNGLSNLRGTVGFARSTEPHSGTSQFYINLADNIDLNPRPTRWGYAVFGKVVEGMEV
                     VDDIGHRPTGGGGSFDRNVPIEPIVIERIEVVE"
BASE COUNT          502 a          936 c          931 g          502 t
ORIGIN      
        1 cgacatcgac ggcttcgaca cctcgtgctt ctccggcgaa tacgtgacgg gcgacgtcag
       61 ccggtcgtac ctcgacgcgc tcgaggtgat tcgttcgaac tccgcgaagg cccgccgcga
      121 cgccaaggtg cgcgccgagg agtcggagga cgacacgatg caggtggcga gcggactcta
      181 gctcaagtcc tttgcgcttt ctcatcatcg acgacgacgc agagtatcgg cagctactgc
      241 gttatcacct cgaggtcgag tggcccgacg ccgtcatcga cgcgcagcaa ccaagctcgg
      301 ccgggcccat tgccgacagc ctcgatctcg ccgacgtcga cctcgtcctc ctgggccatc
      361 ccatggccgc gcacgaagga ttgagtttcc tgcgcctctt gcgccagcgc gacgattgcc
      421 cgccgatatt gctgttcgcg gcgcaaagcg acgagttcct ggccgtcgac gcgttgaagt
      481 ccggagcggc gaatttcttc ccgaaggatc gcgtcaagca tcagcggctc atcgaagtcg
      541 tgcgcggcga gttgcgcgtc gaacgtctcg acccaacggg cgagctgctc gtcgaccaca
      601 aagcgttgaa cggcgcacgc aaacgccggc tcgtcgcgaa gctgtacgcc ggcgatctgt
      661 cgtcggtcta tctggcggag tcggaagagg gcggtgagcg tatcgcgctg aaggtattgc
      721 ggcacgtccc cgacgtcggg agcggccgct tgttcgaccg cttcttgcaa gaatacgaaa
      781 tcatcgcgcg cgtgcaccat gcgaatgtcg tgcggatctt cgacctcggc gtggccgacg
      841 atcatgcgta catcgcgatg gagtatctcg cagcgggcag tctcgccgag cgcctgaagc
      901 gagcgctcga gcccacgacg acggccgatt acatacggca gatcgcggga gcgctcgccg
      961 cgatccacgg cgccggcatc ctgcatcgcg atctgaagcc ggccaacatc atgtttcgcg
     1021 acgacggcag cctcgccttg atcgacttcg ggcttgcgaa gcagatgcgc ctgcatgccg
     1081 cgatcacggg cacggggcag attttcggca cgccgtacta catgagcccg gagcaaggcc
     1141 acgccgagcc gactgacgag cgcagcgatc tgtatagcct cgggtgcatc ttctacgaga
     1201 tgttgaccgg cgagcggccc ttcacggcga gctcgccgat gggagtcatc taccgccacg
     1261 cccatgcgcc gcgaccgcgc ttgagtcgcg ccttgacgca ctatcagccg ctgctcgatc
     1321 ggctactcgc cgtcgaccgg tacgagcgct atcaatcggc cggcgagctg ctcgcggcgc
     1381 tcgaagcgca gctgcgctga cggcaaacgc gtcagccgat cgtctcgagc gcgaatccgt
     1441 cggcggtcca acgcacgacg ctggcctgct cgtaccacgc gccgagcacg atccgcgcgg
     1501 ccggcttacc gtccaagccg aagcggtgca cggcggggcg atgcgtgtgc ccgtgcagga
     1561 gcagtgtgac gtcgaactcg cgcatcgtgc gttcgacggc ctgttgattg acgtccatga
     1621 tgtaggcgct cttcgccgca atttccgtcg cggatagctg gcgcaaatcg ctggcgatct
     1681 ggcggcgctc ggccagcgac ttcgcgagaa actcgcgttg ccagatcggc gagcgcagct
     1741 cggaacgcaa cgtcaggtag cgcgtgtcgt cggtgcagag caggtcgccg tgcgtcagca
     1801 gcacgctctc gccgaacagc gtcacgcgct cgtagtcgcc gagcagacgg caacccgtcg
     1861 ttgcgcagaa gcgctgcccc agcaagaaat cgcgattgcc gtgcatcaac ccacagggga
     1921 taccggcggc cgtgagccgt gtgaccgctg cgacgatcgg tgcgaggcgc gggtcgtcgt
     1981 cgtcgtcacc gatccaagcc tcgaacaggt cgccgaggat gtagagctcg gtcgccgcgc
     2041 gcgcgggacc gtccatgaag tcgacgaagc ggcgcgtggt ctccgtatcg cccgcagtga
     2101 ggtgcaggtc ggagatgaac agcaccgtca tgaacgctac tcgacgactt cgatgcgctc
     2161 tatgacgatc ggctcgatgg gcacgttgcg gtcgaacgag ccgccgccgc ccgtcggccg
     2221 atgaccgatg tcgtcgacga cttccatgcc ttcgacgacc ttgccgaaga ccgcgtagcc
     2281 ccagcgcgtc ggccgcgggt tcaggtcgat gttgtcggca aggttgatgt agaactggct
     2341 cgtgcccgag tgcggctccg tgctgcgcgc gaagccgacc gtgcctcgca gattgctcaa
     2401 gccgttgccc gactcgttga agatgttcga ggttacgtct ttctgcttca tatcggccgt
     2461 gaagcccccg gcttgcgcga tgaagcccgc cacgacgcgg tggaagatca cgccggagta
     2521 aaagccttgg ttcacgtact tcaagaaagc ttcgacggtc agcggcgagc ggcccttatc
     2581 gagctcgatg acgaagttgc cggcattcgt aaccacgcgg acccgcggtg cgacggtgct
     2641 cgccggcgtg gatgcgtctt gagcgcccgc gacctgcagc gtcaggagca ggcagagcag
     2701 caggcacttt ccgcgcatat tcgacctcga gtcttttgcc ttggttacga ccgcttaaga
     2761 ctgtgccgtg ttccgcgcaa gctcagcgga tacgcaattc ggccgcccgg acggtgtttg
     2821 ccatcagcat ggcgatcgtc agcggcccga ctccgccggg gaccggcgtc a
//
LOCUS       DATLEE010000147         3645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_155683, whole genome shotgun sequence.
ACCESSION   DATLEE010000147 DATLEE010000000
VERSION     DATLEE010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155683"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1832
                     /locus_tag="VM692_03150"
     CDS             <1..1832
                     /locus_tag="VM692_03150"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HUQ51191.1"
                     /translation="TLDGNRMYFTAADIASFNRLRYELDDRTRTGDLQPVFDIFNLFR
                     KRAGERVEGAIALLATEPDYTVDETFLWDRSELPWPTSDSEINEVWRKNVKSDALRLM
                     LTGKTWTEAAEVLKERYERSYKRITQLTTDDVFETYMNAVAHNMDPHSSYLSPRQSEE
                     YRIQMSLSYDGIGASLQLEDDYVKVMNIIPGGPAQIDGQLKQEDRITAVGEGGSEPVD
                     VIGWRLDDVVQKIRGPGGTRVRLQILPAGAAPGSPEKTIELVRDKIKLEEQAAKKELL
                     EIPFDGHNYRIGVINVPSFYQDFAARSRGDDDYTSTSRDVTRLIGEFKAEGGVDGIVM
                     DLRANGGGHLSEATELSGLFIERGPVVQLRETRGTIQQLDDPSPTAIYAGPLAVLVDR
                     YSASASEIFAAAIQDYDRGVIVGQQTFGKGSVQNLFPLDRLMRGTDNGQLTLTIGKYY
                     RVTGESTQHRGVIPDIELPSMVDTATVGESTRDTALPWDRIQPTRFRADPTLASELPD
                     LRAHQQLRAANDPEFSYLLSDVAAVKEINSQKSVSLNLEARVAENKRLEEGRLSRENA
                     RRSALGLEPLTGIEQLDKAEASDAILLRQAARIVAEIAEKEKP"
     gene            complement(1836..2882)
                     /locus_tag="VM692_03155"
     CDS             complement(1836..2882)
                     /locus_tag="VM692_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51192.1"
                     /translation="MLSALLERQGDGPTEELLKLYWNRADVKREVKALRRERFELLDK
                     LKEQEGAILRAQEQLEGLERLLTNPLAAANAMVYFQLRHLWRVGAQRLEQFGRELQTQ
                     REKRERAQLHDAETAKRQRRLDAVKEKMAGLAEKRRGALDEQTRLEQKLASMNSILRL
                     FRAGSLKRQIAGLQSGKQMLEEKLEEMRALTEKIQGEPLPEPEGLSLDSRRLINTAII
                     ALAQHLVVHFAEHDLASLAKTSTERAVADMKFGDRRDCDRMVERIREKIEELKQQKTF
                     ADQVKRRTDLLLTEVKYRNDTDSVPGVESLQVISRTPGAQVSELELARRTSDAPLRIN
                     VVADDYWDLMAVMR"
     gene            complement(2979..3452)
                     /locus_tag="VM692_03160"
     CDS             complement(2979..3452)
                     /locus_tag="VM692_03160"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HUQ51193.1"
                     /translation="MVVDSLLVPQCVYRPGTFTGLMTLYESNYIKLHQLATPLEWPVN
                     ALVSSSPRDSDLHAEIVRREAYTTTLRLTYWFHDAGGVSAPDPDIVVRIYHDARLVEA
                     VSSREAYRHTKLRELASSSSAELDRRWRLNMLLNKWLDYLFEVGHSLSPSERALS"
BASE COUNT          699 a         1179 c         1140 g          627 t
ORIGIN      
        1 acacgctcga cggcaaccgc atgtatttta cggcggccga tatcgcaagc ttcaatcgct
       61 tgcgctacga gcttgacgac cgcactcgca ccggcgacct gcaacccgtc ttcgacatct
      121 tcaatctgtt ccgcaagcgc gccggcgaac gcgtcgaagg cgcgattgcg ctgcttgcaa
      181 ccgagcccga ctacacggtc gacgaaacgt tcctgtggga tcgcagcgag ctgccgtggc
      241 cgacgtccga cagtgagatc aatgaagtct ggcgcaagaa cgtcaagagc gacgcgctgc
      301 gtctcatgct gaccggcaag acgtggaccg aggcggccga ggtgttgaag gagcgctacg
      361 agcgttcgta caagcgcatt acgcaattga cgacggacga cgtgtttgag acgtacatga
      421 acgccgtcgc gcacaacatg gacccgcact cgagctatct gtcgccgcgg cagagcgagg
      481 agtatcggat tcagatgagc ttgtcgtacg acggcatcgg cgcttcactg cagctcgagg
      541 acgactacgt caaggtcatg aacatcattc ccggcggtcc cgcgcagatc gacgggcagt
      601 tgaagcaaga ggatcgcatt acggccgtcg gcgaaggcgg cagcgagccc gtcgacgtga
      661 tcggctggcg gctcgacgac gtcgtgcaga agatccgcgg ccccggcggc acgcgcgtgc
      721 gactgcaaat attgccggcc ggcgccgctc cgggctcgcc cgagaaaacc atcgagctcg
      781 tccgcgacaa gatcaagctc gaggaacagg cggcgaagaa agagctactc gagattcctt
      841 tcgacggcca taactaccgc atcggcgtca tcaacgtccc gagcttctat caggacttcg
      901 cggcgcgcag ccgcggcgat gacgactaca cgagcacgtc gcgcgacgtc acgcgcctca
      961 tcggcgaatt caaggctgaa ggcggggtcg acggaatcgt gatggacctg cgcgccaacg
     1021 gtggcggcca tttgtcggaa gcgacggagc tcagcggcct gttcatcgag cgcggccccg
     1081 tcgtccaact gcgcgagacg cgcggtacga ttcaacagct cgacgatccg agcccgacgg
     1141 ccatttatgc cggtccattg gcggtgctcg tcgaccgcta tagcgcgagc gcgtcggaga
     1201 tcttcgcggc ggcgattcaa gactacgacc gcggcgttat cgtcggccag caaacgttcg
     1261 gcaagggctc agtgcagaac ctgttcccgc tcgaccgcct catgcgcggc accgacaacg
     1321 gccagctgac gctgaccatc ggcaagtact atcgcgtgac cggtgaaagc acgcagcacc
     1381 gcggcgtgat ccccgacatc gagctgccct cgatggtcga cacggccacg gtcggcgaaa
     1441 gcacgcgcga cacggcactg ccatgggatc ggattcagcc gacacgcttc cgcgcagacc
     1501 cgacgttggc cagcgagctc ccggatctgc gagcgcatca gcagctgcgt gcagccaacg
     1561 atccggagtt cagctacctg ctgagcgacg tcgccgccgt caaggagatc aactcgcaaa
     1621 agagcgtctc gctgaacctc gaagcgcgag tcgccgagaa caagcggctc gaagagggcc
     1681 ggctctcgcg cgagaacgcg cgtcgctcgg cactcgggct cgagccgctc acgggcatcg
     1741 agcagctcga taaagcggaa gcgtcggatg cgatcctgct gcgtcaggcg gcgcggatcg
     1801 tcgctgagat tgcggagaag gaaaagccgt agcgctcagc gcatgacggc catcaggtcc
     1861 cagtagtcgt cggcgaccac gttgatgcgc agcggggcgt cgctcgtgcg gcgcgccagc
     1921 tcgagctcgc tgacctgcgc gcccggcgtg cggctgatga cctgcaacga ctcgacaccc
     1981 ggaacggagt cggtgtcgtt gcgatatttg acctcggtaa gcagtaaatc cgtccgccgc
     2041 ttcacttgat cggcgaacgt tttctgctgc ttcagctcct cgatcttctc gcgaatccgc
     2101 tccaccatgc gatcgcagtc acggcggtcg ccgaatttca tgtcggcaac ggcccgttcc
     2161 gtcgacgtct tggccagcga cgccaaatcg tgctccgcga aatgaaccac gaggtgctgc
     2221 gcaagcgcga tgatcgcggt attgatgagc cggcggctgt cgagcgagag cccctcgggc
     2281 tccggcaacg gctcaccttg aatcttctcg gtgagagcac gcatttcctc gagcttttcc
     2341 tcgagcatct gcttgccgct ctgcaagcct gcgatctgcc gtttcaacga gccggcgcgg
     2401 aacaagcgca agatgctgtt catgctcgcc agcttctgct cgaggcgagt ttgctcgtcg
     2461 agcgcgccgc ggcgcttctc cgcgagcccg gccatctttt ccttgaccgc gtcgagccgc
     2521 cgctgacgtt tggccgtctc cgcgtcgtgg agctgcgcgc gctcgcgctt ctcgcgctgc
     2581 gtttgcagct cgcggccgaa ttgctcgagc cgctgcgctc caacgcgcca caagtgacgc
     2641 agctggaagt agaccatggc attcgcggct gcgagcgggt tcgtcagtag ccgctcgagg
     2701 ccttcgagct gctcctgggc gcgcaagatt gctccctctt gctccttgag cttgtcgagc
     2761 agctcgaagc gctccctacg cagggctttg acttcgcgct tgacgtcggc ccggttccaa
     2821 tagagcttca gcagctcctc ggtcgggccg tctccttggc gctccaagag ggccgagagc
     2881 atcagcagat tctgtaagac gaacgcatgc ggcgaaatat agctggcgaa acgcagccaa
     2941 accgtgactt aaagcacgga tcggcgcggc ggcgacgact aactcagcgc gcgttccgac
     3001 ggactcaagc tgtgaccgac ttcaaagagg tagtcgagcc acttgttgag cagcatattg
     3061 agccgccacc ggcgatcgag ctcggcgctg ctgctcgaag ccagctcgcg aagcttggtg
     3121 tggcggtagg cctcacggct actgacggct tcgaccagcc gcgcgtcgtg atagatacgc
     3181 accacgatgt cggggtccgg ggccgacacg ccgccggcgt cgtgaaacca gtacgtcaga
     3241 cgcaaagtcg tcgtataggc ctcgcgacgc acgatctcgg cgtgcagatc gctgtcacgc
     3301 ggcgacgacg agacgagcgc attgaccggc cactcgagcg gcgtcgctag ctgatggagc
     3361 ttgatgtagt tgctctcgta caaggtcatc agccccgtga atgtgccggg gcggtagacg
     3421 cactgcggaa ccagtaggct atcgacgacc atgcgcgtta cagtgtccta aactcgcgcg
     3481 tcaggcaacc aggtcaccaa tccgagcggc gcttgcgacg caagctcggc gcgactagcc
     3541 cgacgacgat gcccgcaagc agcaccgcag cgccgacgac aaaccaattc tgattgttgc
     3601 ggcccgcaag cctcgcgttc tcggccgtca actcctcgac ctcgc
//
LOCUS       DATLEE010000148         5993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_900760, whole genome shotgun sequence.
ACCESSION   DATLEE010000148 DATLEE010000000
VERSION     DATLEE010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900760"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VM692_03165"
     CDS             <1..1001
                     /locus_tag="VM692_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HUQ51194.1"
                     /translation="DAFAPNALDFDSAEIGEPDLGHIVEDALLRTVLGDTVARAPQAQ
                     LQVGSEIASVAVEEREVVVALGQGGNVPLRGLPRVVRGTVLLAADGSDSAVRRLVDLP
                     TTGHRYEQTAIVAHVTSTGSPQSTAWQRFLPGGPLALLPLADGRSSIVWSLPTADAQR
                     LLAASDAEFIAELAVATAGALGELTACSKRVAFPLQALHALKYATERVALLGDAAHTV
                     HPLAGQGMNLGLLDAASIAAVIEDALLAGEDVGDLKVLRRYERQRKGDNLAMLAAFDG
                     LNRLFRLPGWAAPLRSLGLRAVEAADPVKRLLMQKALGLTAGRKHRIRWSRAESQA"
     gene            1013..3820
                     /locus_tag="VM692_03170"
     CDS             1013..3820
                     /locus_tag="VM692_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUQ51195.1"
                     /translation="MSKFRVVTRGALLALTLIAAAPLAAQEQTWNRTLERIASGVVSI
                     QIDSTRSFDTERNQSSQATGFVVDAERGLILTNRHVVTPGPVRAQAVFLNQEEVELVP
                     VYRDPVHDFGFFRYNPKDLQFMKPTELKLDPTAAQIGRDIRVVGNDAGEQLSILAGTI
                     ARLDRQAPNYGRGNYNDFNTFYLQAASGTSGGSSGSPVVDIEGRVVALNAGANSQAAS
                     SFFLPLDRVQVALADIQKGEYVPRGTLQTEFVHKPFAELRRLGLTRDTEARVRQANAQ
                     QTGMLVVEQVIPDSAASRALEPGDILVEVDGKLIAEFVPLAAVLDGAVAREVDVLVER
                     GGQRLEHKIKVDDLHGITPDEYIEYGDGVFHNLSYQQARHFYRPVAGVYVANPGYVFG
                     KAAIPRGSIVTGIGSASLHNLDDLERVLESLPDDAQAPVRFVTFDEPTTERQRLITND
                     RSWVPAQRCHRDDKVGVWPCQALAPSPPREPMTPSEGTTFPRQGERHVQAVTPSLVMV
                     NFDMPYTVSGVADRYYYGTGLIADAVRGLVVVDRNTVPVAMGDVRLTFAGSLEIPGRV
                     EYIHPLHNLAVVSYDPKLIGNTPVKSAVFLNKPLLPGDDVAVVGMGPDFKMLSQSSEV
                     ANVSAASFPLSRTLRFRETNLDVATLVNGPSDFDGTLVDFQGRVLGMWASFAYQSGRD
                     LTQVNLGIPADVIIDMIDKLRAQEPLRSIEVEWAQMPLATARKVDLPEEWIQRYEAHN
                     PAKREVLTVATTVADSPASQFFRSGDILLSIDGVLANTFRQAERASQKPTVDVTVFRN
                     GQEVKGSVGTVTIDGLGIERVVSWAGALIQAPHRELAVQRGVGTGGVYVSFFNFGSPA
                     SRSGLFAGRRIVAVDGEPTPDLEAFVRVATSVGDKDSVRLNTMSFNDVPEVITMKLDP
                     QYWPSYELVREGYEWRRQALN"
     gene            3838..4260
                     /locus_tag="VM692_03175"
     CDS             3838..4260
                     /locus_tag="VM692_03175"
                     /inference="COORDINATES: protein motif:HMM:NF039479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6524 family protein"
                     /protein_id="HUQ51196.1"
                     /translation="MAAPAIGLAGVGLRFVFAFALVVLTWNPTRYNFVAWAQTQWDGL
                     APLVLFVGIVLLMGWIFFVRTAAKSLGGLGLILCLALAGTVVWTLFYFDLVTGDSRNL
                     LSWIVLVLFSAILAAGMSWAHLRNRWSGQATVDEVDNR"
     gene            4353..5297
                     /locus_tag="VM692_03180"
     CDS             4353..5297
                     /locus_tag="VM692_03180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUQ51197.1"
                     /translation="MILRASLTAAARGLLLAALAFPAAGQKLYRYKDENGVSVYTDRR
                     PGGEQTFDEQQVERRFEKPEIRLLQRSARGGVALIAQNTFFAPIQIAFRLNAVENMSA
                     DTPRTGLKVLPPRSETELVVVGKAVQQVDLRFDSEFQYLPGEPGAEHRPTQPYRLPYA
                     LSTSVRVSQAFPDKKTHTDPASQHAVDFVMPIGTDIFAARDGVVIEVASDFFESGTDL
                     QVDGPRANIVRVLHDDGTMALYVHLNWNTIRVVPGQHVRRGEYLADSGNTGFTTGPHL
                     HFVVQRNQGGSLVSVPLQFADATGAPISVASGQSYTAY"
     gene            complement(5294..>5993)
                     /locus_tag="VM692_03185"
     CDS             complement(5294..>5993)
                     /locus_tag="VM692_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUQ51198.1"
                     /translation="SATRAVDFIRAELWRDGRLKATFKDGRARFAAYLDDYAFLAYGL
                     VELLQTRWRDSDLTLARQLADVMLQHFEDPRGGFFFTADDHERLIHKPKPVTDESVPA
                     GSAVAALALSTLGHLLGEQRYLDAVERTVRGALYGIEHYAEAHPTMLRVLEHVLTPPK
                     LVVLRGSAEELAPWQRQLGRSYDPHRLAFAIPNDAVLPGLLAAREPLEHAVAYVCEGM
                     TCRAPVELKDGVAL"
BASE COUNT          959 a         2035 c         1995 g         1004 t
ORIGIN      
        1 ccgacgcgtt cgcgccgaac gcgctcgact tcgactccgc cgagatcggc gagcccgatc
       61 tcggccacat cgtcgaggac gcgctgctgc ggaccgtgct cggcgacacg gtcgcgcgcg
      121 cgccgcaggc gcagctgcaa gtcgggtcgg agatcgcgtc cgtcgcggtc gaggagcgcg
      181 aggtcgtcgt cgcgctcggc caaggcggca acgtccccct tcggggactt ccaagggtcg
      241 tgcgcggcac cgtgctgctg gccgccgacg gcagcgactc ggccgtgcgg cgcctcgtcg
      301 atctgccgac gacgggccac cgctacgagc agaccgcgat cgtcgcacac gtgacgagca
      361 cgggttcgcc tcagagcacg gcgtggcagc ggttcctgcc cggcggaccg ctcgcgttgc
      421 tgccgctcgc cgacggccgt agctcgatcg tctggtcgct gccgacggcc gacgcgcagc
      481 gcttgctcgc ggcgagcgac gccgagttca tcgccgagct tgcggttgcc acggccggcg
      541 ctctcggcga gctcacggcg tgctcgaagc gcgtcgcctt cccgttgcaa gcactgcacg
      601 cactgaagta cgcaactgag cgcgtcgcct tgctcggcga cgctgcgcac acggtgcatc
      661 cgctcgcggg gcagggcatg aacttaggac ttcttgatgc agcgtcgatc gcggccgtca
      721 tcgaggacgc actgctggcc ggagaggacg tcggcgatct caaagtactg cgccgctacg
      781 agcgccagcg caagggcgac aatctcgcga tgttggctgc tttcgacggt ctaaaccgcc
      841 tgttccgctt gccgggctgg gccgcgcccc tgcgctcttt gggcctgcgc gcggtggagg
      901 cggcggatcc cgtcaaacgc ttattgatgc agaaggcctt gggtcttacg gccgggcgca
      961 agcaccgtat tcgctggtca cgcgcagagt cgcaagcata gaatccgccg ctatgagcaa
     1021 attcagagta gtgactcgcg gggcgctgct cgcgcttacg ctgatcgcgg ccgcgcccct
     1081 cgcggcgcaa gagcaaactt ggaatcgcac gctggagcgc atcgcctccg gcgtcgtctc
     1141 gattcaaatc gatagcacgc gctcgttcga taccgagcgc aaccaatcga gccaagcgac
     1201 aggcttcgtc gtcgacgccg agcgcggctt gatattgacg aaccggcacg tcgtgacgcc
     1261 gggccccgtg cgcgcgcaag ccgtgttcct caatcaagag gaagtcgagc tcgtgcccgt
     1321 ctatcgcgac cccgtgcacg acttcgggtt cttccgttac aacccgaaag acctgcaatt
     1381 catgaagccg accgagctca agctcgatcc gacggcggct cagatcggcc gcgacatccg
     1441 cgtcgtcggc aacgacgccg gcgagcagct gtcgatcctc gcgggcacga tcgcgaggct
     1501 cgatcgccag gcgccgaatt acgggcgcgg caactacaac gacttcaaca ccttttatct
     1561 gcaagccgcg tcgggcacct cgggcggatc gtcgggctcg cccgtcgtcg acatcgaagg
     1621 ccgcgtcgtc gcgctgaacg ccggtgcgaa ctcgcaggcc gcgtcgagct tcttcctgcc
     1681 gctcgaccgc gtccaggtcg cgctcgccga catccagaag ggcgagtacg tcccgcgcgg
     1741 cacgctgcaa accgagttcg tccacaagcc gttcgccgag ctgcggcgcc tcgggctgac
     1801 gcgcgatacg gaagcgcgcg tgcgccaggc caacgcgcaa cagaccggca tgctcgtcgt
     1861 cgagcaggtg attccggact ctgccgcgtc acgcgcgctc gagcccggcg acatcctcgt
     1921 cgaggtcgac ggcaagctga tcgcggagtt cgtgccgctg gcggccgtcc tcgacggcgc
     1981 cgtcgcgcgc gaggtcgacg tcctcgtcga gcgcggcggg cagcggctcg agcacaagat
     2041 caaagtcgac gatctgcacg ggatcacgcc cgacgagtac atcgagtacg gcgacggcgt
     2101 gttccacaac ttgtcttatc agcaggcccg gcacttctac cggcccgtgg cgggcgtcta
     2161 cgtcgcgaac cccggctacg tgttcggcaa ggccgcgatc ccgcgcggca gcatcgtgac
     2221 gggcatcggc agcgcttcgc tgcacaatct cgacgatctc gagcgcgtgc tcgagtcgct
     2281 gcccgacgac gcgcaagcgc ccgtgcggtt cgtgacgttc gacgagccaa cgaccgagcg
     2341 gcagcggctc atcacgaacg accgcagctg ggtcccggcg cagcgctgcc atcgcgacga
     2401 caaggtcggc gtgtggccgt gccaggcctt ggcgccgagc ccgccgcgcg agccgatgac
     2461 tccgagcgaa ggtacgacgt ttccgcgcca aggcgagcgc cacgtgcagg ccgtgacgcc
     2521 gtcgctcgtg atggtcaact tcgacatgcc gtacacggtg tccggcgtcg cggatcgcta
     2581 ttactacggc acgggcctca tcgccgacgc ggtgcgcggg ctcgtcgtcg tcgatcgcaa
     2641 cacggtgccc gtcgcgatgg gcgacgtgcg cctgactttt gcgggctcgc tcgagattcc
     2701 gggccgcgtc gagtacatcc atccattgca caaccttgcg gtcgtgtcgt acgaccccaa
     2761 gttgatcggc aacacgccgg tcaagagcgc cgtgttcctg aacaagccgc tgctgcccgg
     2821 cgacgacgtc gccgtcgtcg gcatggggcc ggacttcaag atgctgtcgc aatcgagcga
     2881 agtcgcgaac gtctctgccg cgtcgttccc gctgtcgcgc acactgcgct tccgcgagac
     2941 caacttggac gtcgcgacgc tcgtcaacgg gccgtcggac ttcgacggca cgctcgtcga
     3001 cttccaaggc cgcgtgctcg gcatgtgggc cagctttgcc tatcagtcgg gccgcgatct
     3061 cacgcaagtg aatctcggca ttccggccga tgtcatcatc gacatgatcg acaagctgcg
     3121 cgcgcaagag ccgttgcgct cgatcgaggt cgagtgggcg cagatgccgc tcgcgacggc
     3181 gcgtaaagtg gatctgcccg aggagtggat ccagcgctac gaagcgcaca atcctgccaa
     3241 gcgcgaggtg ctgaccgtcg ccacgacggt cgccgactcg ccggcctcgc agttcttccg
     3301 ctccggcgac atcctgctca gcatcgacgg cgtgctcgcc aacacgttcc gccaagccga
     3361 gcgagcttcg cagaagccga cggtcgacgt taccgtgttc cgcaacgggc aagaggtcaa
     3421 gggcagcgtc ggcaccgtta cgatcgacgg gctcggcatc gagcgcgtcg tttcgtgggc
     3481 cggcgcgctg attcaggcgc cgcaccgcga gctcgccgtg cagcgcggcg tcggcaccgg
     3541 cggcgtctac gtgagtttct tcaacttcgg ctcgccggcg agccgctcgg gattgttcgc
     3601 cggccggcgc atcgtcgcgg tagacggtga gccgacgccg gatctcgaag cgttcgtccg
     3661 cgtcgccacg agcgtcggcg acaaagactc cgtgcgcctc aacacgatgt cgttcaacga
     3721 cgtccccgag gtcatcacga tgaagctcga cccgcagtac tggccgagct acgagctcgt
     3781 gcgcgaaggg tacgaatggc ggcgtcaggc cctgaactga cgctctagga gaacgtcgtg
     3841 gcagcacccg caatcggtct cgccggcgtc ggcttgaggt ttgtgttcgc gttcgcgctc
     3901 gtcgtgctga cgtggaaccc gacgcgctac aacttcgtcg cctgggcgca aacgcaatgg
     3961 gacggattgg cgccgctcgt gctattcgtc ggcatcgtgc tgctgatggg ctggatcttc
     4021 ttcgtgcgga cggcggcgaa gtcgctcggc gggctcggcc tgatcctctg cctcgcgctg
     4081 gcgggcaccg ttgtctggac gttgttctat ttcgacctcg tgaccggcga cagcaggaat
     4141 ctgctctcgt ggatagtgct cgtgctgttc agcgcgattc ttgcggccgg catgtcgtgg
     4201 gcgcatctgc gcaaccgctg gtcgggtcag gcgaccgtcg acgaggtcga caaccgctaa
     4261 ggacgcctgt cgcggcggtt tctctcgcca agctcggcct tattgtgaaa agagtccttg
     4321 gccgtctggt gacgatcttg cacagttccc tcatgattct tcgggcctcc ttgaccgcgg
     4381 ccgcgcgcgg gctgttgctc gcggcgctcg catttcccgc cgcgggccag aagctgtatc
     4441 ggtacaagga cgagaacggc gtctccgtct acacggatcg gcggcccggc ggcgagcaga
     4501 ccttcgatga gcagcaggtc gagcggcggt tcgagaagcc cgagatccgg ctgctgcagc
     4561 gctcggcgcg cggcggcgtc gcgttgatcg cgcagaacac cttcttcgcg ccgattcaga
     4621 tcgctttccg tttgaacgct gtcgaaaaca tgtcggcgga cacgccgcgc acggggctca
     4681 aggtcttacc gccgcgcagc gagacggagc tcgtcgtcgt cggcaaagcc gtccagcagg
     4741 tcgacctgcg cttcgactcg gaattccagt atctgcccgg cgagcccggc gccgagcatc
     4801 ggccgacgca gccgtaccgg ctgccgtacg ccttgtcgac gtcggtccgg gtgtcgcaag
     4861 cgttcccgga caagaagact catacggatc ccgcgagcca gcatgccgtc gatttcgtga
     4921 tgccgatcgg taccgacatc ttcgcggcac gcgacggcgt cgtcatcgag gtcgcgtccg
     4981 acttcttcga gagcggcacc gacctacagg tcgacggtcc gcgcgcgaat atcgtgcgcg
     5041 tgctgcacga cgacggcacg atggcgttgt acgtgcatct gaactggaac acgatccgcg
     5101 tcgtgccggg gcagcacgtg cgccgcggcg agtatctcgc ggactcgggc aacacggggt
     5161 ttacgacggg accgcacttg cacttcgtcg tgcagcgcaa tcaaggcggg tcgctcgttt
     5221 ccgtgccgct gcagttcgcc gatgcgaccg gcgcgccgat cagcgtcgcg agcggccaga
     5281 gctataccgc gtactagagc gccacgccgt ccttgagctc gacgggcgcg cggcacgtca
     5341 tgccctcgca aacataagcg acggcatgct cgagcggctc gcgtgccgcg agcagccccg
     5401 gcagcaccgc atcgttcggg atcgcgaacg cgaggcgatg cgggtcgtaa ctgcgcccca
     5461 gctgtcgctg ccacggcgcg agctcctcgg ccgagcctcg caagacgacg agcttcggcg
     5521 gcgtcaagac gtgctccaac acgcgcagca tcgtcggatg cgcctccgcg taatgctcga
     5581 tcccgtacag cgccccgcgg accgtgcgct cgacggcatc gaggtagcgc tgctcgccga
     5641 gcaagtggcc gagtgtcgac aatgcaagcg ccgcgacggc gctgccggcc ggcacggact
     5701 cgtcggtgac gggcttcggc ttgtggatga gccgctcgtg gtcgtcggcc gtgaagaaga
     5761 acccgccgcg cggatcctcg aagtgctgga gcatgacgtc ggcgagctgc cgcgcgagcg
     5821 tgagatcgct gtcgcgccac cgtgtctgca gcagctcgac gagcccgtaa gccaagaacg
     5881 cgtagtcgtc gagatacgcc gcaaagcgcg cgcgcccgtc cttgaacgtg gccttgaggc
     5941 gaccgtcgcg ccagagctcg gcacggatga agtcgacggc gcgcgttgcg ctc
//
LOCUS       DATLEE010000149         3432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4622399, whole genome shotgun sequence.
ACCESSION   DATLEE010000149 DATLEE010000000
VERSION     DATLEE010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4622399"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VM692_03190"
     CDS             <1..1166
                     /locus_tag="VM692_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HUQ51199.1"
                     /translation="GYAVATSPPVYAVIRKLAAWPTTGRGACAYVGLFSMLASLVSWS
                     FSLIFSALLAREIAHRVRGSDYRAIGAAAYLGVGSVWALGMSSSAALIMANPASLPAS
                     ISRITGTIPLSQTIGLWQSLLIAAVLIVVSMIVSYHSAPGPAQAKSMADMGVTYAPAT
                     NDIGRRSTPGEWLEFSPLLTIVICVLGFGYLAREVMASGVGVLLDLNHYLFLFLMLGL
                     LLHWRPKSFVQSIAASVTPVGGVLIQYPMYAGILEMLTQSGLAKNISHFFVYISNEHT
                     FPVLVGIYSAFLGLFIPSAGGKWLLEAQYLLDAAKQHEVHLGWVVQTYNATEALANLI
                     HPFWMLPLVGILGLKARDIVGYSMLQFVVHVPLVLLLVWLLNYTLTYVPPVLP"
     gene            1210..1878
                     /locus_tag="VM692_03195"
     CDS             1210..1878
                     /locus_tag="VM692_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51200.1"
                     /translation="MSHEAAAPRDRRFYAAMATIIAALVFAGFTPSFYTRSSGLEPLP
                     APVVVHGVAGTLFVLVFAVQSWLIAVGREGAHRKLGVGGAALAAAFVISGVAVTVRLE
                     ESHAFDTTRVLAAHVWTNAAPLAAFALLVAAGIWQRHVAARHKRLMLLAAVVLLPPAT
                     GRLFGPLELAWLNLPLYVCAAAASSVYDVVTRGRPHALSVFPAAALVAIDVTTTWWLS
                     AVGS"
     gene            1952..2311
                     /locus_tag="VM692_03200"
     CDS             1952..2311
                     /locus_tag="VM692_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HUQ51201.1"
                     /translation="MEKHRVFAMKFAKVYPLYVQKAERKNRTKQDVDEIICWLTGYSH
                     AQLRRQIERESDFATFFAQAPAINPNVSLIKGSVCGVRLEEIDDPLMKHIRYLDKLID
                     ELAKGKPMEKILRQQAI"
     gene            2308..2622
                     /locus_tag="VM692_03205"
     CDS             2308..2622
                     /locus_tag="VM692_03205"
                     /inference="COORDINATES: protein motif:HMM:NF025161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HUQ51202.1"
                     /translation="MSSDPQRPSVASSRDIGDVLRRSLAPTGCVLAEEELGPEFFDLR
                     TGLAGELFQKIVNYRGRLAIVIRDARVHGDRFAELAYEHRNHPAVRFFTEHTEARRWL
                     GG"
     gene            complement(2611..>3432)
                     /gene="rlmJ"
                     /locus_tag="VM692_03210"
     CDS             complement(2611..>3432)
                     /gene="rlmJ"
                     /locus_tag="VM692_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HUQ51203.1"
                     /translation="LHGYHAGNHADVLKHTVLTAVLARLVAKDKPLRYVDTHAGAGGY
                     DLRAATAQRNREHEGGVGKLWVAADAPPAVARWLALAHRYNGDGALRRYPGSPWFAAQ
                     ALRASDDLFLFELHPAEHRALKKNLDGDRRIKLLRADGLAACIGLVPPPAKRALVLID
                     PSYELRDEHRAVIDSVVKLHKRFATGTIAIWYPVIERRWVERYERALRAAGIGAFATY
                     ELCVAHERRGGGLTGSGLFVLNPPWQLDDELETALPWLVQRLGVDDGAGFRLLSA"
BASE COUNT          536 a         1083 c         1158 g          655 t
ORIGIN      
        1 cgggctacgc ggtcgcgacg tcgccgcccg tgtatgccgt gatccgcaag ctcgcggctt
       61 ggccgaccac gggtcgcggc gcgtgcgcat acgtcgggtt gttctcgatg ctcgcgtcgc
      121 tcgtgtcgtg gagcttcagc ctgatcttca gcgcgctgct ggcgcgcgag attgctcatc
      181 gcgttcgggg ctcggactac cgcgcgatcg gcgccgccgc gtacctcggc gtcggcagcg
      241 tctgggcact cggcatgtcg tcgtcggccg cgctgatcat ggcgaacccc gcgtcgttgc
      301 cggcttcgat cagccgtatc acgggcacga ttccgttgag ccagacgatc ggcttgtggc
      361 agagcctttt gattgccgcc gtgctgatcg tcgtctcgat gatcgtgtcc taccattccg
      421 cgccggggcc cgcgcaggcg aagtcgatgg cggacatggg cgtgacgtat gcgcccgcga
      481 cgaacgacat cggccggcgc tcgacgcccg gcgagtggct cgagttcagc ccgctgctga
      541 cgatcgtgat ttgcgtgctc ggcttcggct atctcgcgcg cgaggtcatg gccagcggcg
      601 tcggcgtctt gctcgacttg aaccactact tgttcctgtt cttgatgctc ggcctgctgc
      661 tgcattggcg gcccaagtcg ttcgtgcagt cgatcgcggc gtcggtgact cccgtcggcg
      721 gcgtactgat tcagtatccg atgtatgccg gcatcctcga gatgctgacg cagtcggggc
      781 tcgcgaaaaa catctcgcac ttcttcgtct atatctccaa cgagcacacg ttccccgtgc
      841 ttgtcggcat ctattcggct ttcctcggct tgttcattcc gtcggccggc ggcaagtggc
      901 tactcgaggc gcagtacctg ctcgatgcgg cgaagcagca cgaggtgcac ctcggctggg
      961 tcgtgcagac ttacaacgcc actgaagcgc tcgcgaattt gattcatccg ttctggatgc
     1021 tgccgctcgt cggtattctc gggctcaaag cgcgcgacat cgtcggttac tcgatgctgc
     1081 aattcgtcgt gcacgtgccg ttggtgttgc tgctcgtctg gctgctgaat tacacgctga
     1141 cgtacgttcc gcccgtattg ccgtgacggc gcgcgcggag cccccgtcgg ggactaccaa
     1201 gggtcgcgct tgagccacga ggctgccgcg ccgcgcgacc gcaggttcta tgcggcgatg
     1261 gcgacgatca tcgccgcgct ggtcttcgcg gggttcacgc cgtccttcta cacgcgctcg
     1321 agcggtctcg agccattgcc cgcgccggtc gttgtgcatg gagtcgcggg cacgttgttc
     1381 gtgctcgtgt tcgccgtgca gtcgtggctc attgccgttg ggcgcgaggg cgcgcaccgc
     1441 aagctcggag tcggcggtgc tgctcttgcg gcggcattcg tgatcagcgg cgttgcggtc
     1501 accgtacgcc tcgaagagag ccacgcgttc gacacgactc gcgtgctggc tgcgcacgtt
     1561 tggacgaacg ccgcgccgct cgcggcattt gcgctgctcg ttgccgccgg aatctggcaa
     1621 cggcacgtgg ccgctcggca caaacggctg atgctgctcg ccgccgtcgt gctgctgccg
     1681 ccggcgacgg gacgcctctt cggcccgctc gagctagcgt ggttgaacct gccgctctac
     1741 gtttgcgcgg ccgcggcgag ctcggtgtac gacgttgtga cgcgcggacg gccgcacgcg
     1801 ctgtcggtgt ttcctgccgc ggctctcgtg gcaatcgacg tcacgacgac gtggtggctg
     1861 agcgcggtcg gctcgtagcg tggcgggggt atgcattacc agcagggaga cgcgtgaagt
     1921 cgtggctccg catttggaca aggactgaga aatggaaaag catcgtgtct tcgcgatgaa
     1981 gttcgcgaag gtgtatccgc tgtatgtgca gaaagcggaa cgaaagaacc gcacgaagca
     2041 agacgtggac gagatcattt gttggttgac cggctacagt catgcgcaac tgcggcggca
     2101 gatcgaacgg gaaagcgact tcgcaacctt ttttgcgcaa gcgcccgcca tcaatccaaa
     2161 cgtttcgctg atcaagggct cggtgtgcgg cgttcgtctg gaagagatcg acgacccgct
     2221 catgaagcac atacggtacc tcgacaagtt gatcgacgag ctcgcaaagg gcaagccgat
     2281 ggaaaagatt ctccggcagc aagcgatatg agcagtgacc ctcaacgacc aagcgtcgca
     2341 tccagtcgag acatcggcga cgttctgcga cgcagtcttg cgccgacagg gtgcgtgctc
     2401 gcggaagaag agctaggccc cgagttcttc gacttgcgca cgggtttggc tggcgagctg
     2461 ttccaaaaga tcgtcaacta ccgcgggagg ctggccatcg tgattcgcga cgcgcgcgtg
     2521 catggcgacc ggttcgccga gctcgcctac gagcatcgca atcatcccgc ggtgcgattc
     2581 ttcacggagc acaccgaagc gcggcgttgg ttaggcggat aagagccgga accccgcgcc
     2641 gtcgtcgacg ccgagccgct ggacgagcca gggcagggcg gtttcgagct cgtcgtcgag
     2701 ctgccacggc gggttcaaga cgaacaagcc gctgccggtg aggcctccgc cgcggcgctc
     2761 gtgggcgacg cagagctcgt aggtcgcgaa cgcaccgatg ccggccgcgc gtaacgcgcg
     2821 ctcgtagcgc tcgacccagc gccgctcgat gacgggatac cagatcgcga tcgtgcccgt
     2881 cgcgaaccgc ttgtgcagct tgacgacgga gtcgatgacg gcgcggtgct cgtcgcgcaa
     2941 ctcgtacgac gggtcgatca gcacgagcgc gcgtttcgcg ggtggcggca cgaggccgat
     3001 gcaagcggcg aggccgtccg cgcgcagcag cttgatccgg cggtcgccgt cgagattttt
     3061 cttcaacgcg cgatgctcgg cgggatgcag ctcgaacaag aacaaatcgt cgctcgctcg
     3121 cagcgcttgc gccgcgaacc aaggcgagcc gggataacgg cgcagcgcgc cgtcgccgtt
     3181 gtagcgatgc gcgagcgcga gccaacgcgc aaccgcgggc ggcgcgtctg cggcaaccca
     3241 gagcttgccg acgccgcctt cgtgctcgcg attccgctgc gcggtggcgg cacggaggtc
     3301 gtagccgcct gcaccggcat gcgtgtcgac atagcgcagc ggcttgtcct tcgcgacgag
     3361 ccgcgcgagc acggcggtca gcaccgtgtg cttcaggacg tcggcatgat tgccggcgtg
     3421 gtaaccgtgc ag
//
LOCUS       DATLEE010000150         3513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_903049, whole genome shotgun sequence.
ACCESSION   DATLEE010000150 DATLEE010000000
VERSION     DATLEE010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903049"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VM692_03215"
     CDS             <1..1275
                     /locus_tag="VM692_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ51204.1"
                     /translation="TTRALRRSKTMALLVLDLDRFKTVNEMLGHEAGDALLREVVQRL
                     TGSIREGDVLARLGGDEFAVLLEEVASPFEAEEIARNIGTALQEPFEVGGRSVSVSAS
                     VGITVCPTDSTDPVALLNNANIAMYRAKEQGLNTVKMFTAAMHEEIISYHRLETDLKD
                     AIARGQFEILYQPQLRLADQRVDAVEALLRWKHPQRGLLSPTDFISVAEESGHIIPLG
                     LWVIEEVARQLKRWESAGVPVPRVAINVSALQFRQPDFHEVLRTALETHSVDPSLIEL
                     ELAEHSLMQDTSGTRDSLRALKSVGVRLAIDDFGAGYSCLSYLRQFPLDVLKIDRSFV
                     SDLDTSLDAQAICSAILSIAHRLSLDAVAEGIETERQLAFLTRNDCLYGQGFYFSAPV
                     EADKIAVLMAQSGAQSTRRRRVTSRRIAAKAG"
     gene            1286..2527
                     /locus_tag="VM692_03220"
     CDS             1286..2527
                     /locus_tag="VM692_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ51205.1"
                     /translation="MTDANRPRPTTLEDALRALDAAEEKLRAQVDDTGIRQLSENAQR
                     SEERFRMLAEGVPNHLLFLDRELRITFANDVFLEAVGWSADKAMGCHISDIMGVERYL
                     ERQPYYERALAGETVSYESTGAAGSEHGYFRFSYRPSFDEAGKVRGIFSMATDISARR
                     KIELELEAKQAELLRSNKDLEQFAYVASHDLKAPLRAIDLLVQWIVEGLAGYDANNVQ
                     ENLGLLGKRTQRLNRLLDDLLAYSRAGRKVGAHRIVDAHALVLDVIQMLNSPATVSVS
                     IEGKLPTFKTHPTPLETVLRNLISNAIKHHPGPQGRVVVSCQEQSDQYVFAVEDDGEG
                     IPPQYAERVFEMFQTLKPRDQVEGSGMGLAIVNRIVQWQSGRVWFEPAPSGRGAVFKF
                     QWKKNQPLAAKVETKAWEAAR"
     gene            2509..2928
                     /locus_tag="VM692_03225"
     CDS             2509..2928
                     /locus_tag="VM692_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUQ51206.1"
                     /translation="MGSSKVVTFLLVEDDEIDVKALKWAFDKLKIANPLKIARDGVEA
                     WEMLQELPRPYLIISDINMPRMNGIELLRKIRQSEHCRDSIVFMLTTSNDEQDKIDAY
                     DLNVAGYMLKSDMGTSFTRAISLIDNYWKVVEFPEHV"
     gene            complement(2944..>3513)
                     /locus_tag="VM692_03230"
     CDS             complement(2944..>3513)
                     /locus_tag="VM692_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUQ51207.1"
                     /translation="GSSHFDQVLNAVMLLSYVALAQGDAVGALTFGTPPGGERLFAPR
                     KGAHVLNALMGELYGLQPTPSHSDYVAAAKDLLRRQNKRALVIVITNFRDEDGSELGL
                     ALKLLRQRHLVLLASVRERIVGELIAQPLTSGNAALDVASAHLYEQARRDAFRRLAAR
                     DALMVDAEPERLGVELVNRYHAVKRAGLI"
BASE COUNT          681 a         1129 c         1114 g          589 t
ORIGIN      
        1 acgacgcgtg cgctgcgccg tagcaagacg atggcgttgc tcgtgctcga tctcgaccgg
       61 ttcaagaccg tcaacgagat gctgggccac gaagccggcg acgctttgtt gcgcgaagtc
      121 gtgcaacggc tcaccggtag cattcgcgaa ggcgacgtgc tggctcgact cggcggcgat
      181 gaattcgccg tgctgctcga agaagtcgcg agcccgttcg aggccgaaga aatagcgcgc
      241 aatatcggca ccgcgctgca ggagccgttc gaggtcggcg ggcgctccgt atcggtcagc
      301 gcgagcgtcg gcatcacggt gtgccccacc gacagcaccg acccggtcgc gttgctgaac
      361 aacgcaaaca tcgcgatgta ccgcgcgaag gagcaagggc tcaacaccgt caagatgttc
      421 acggcggcga tgcacgaaga gatcatttct tatcatcggc tagagacgga cttgaaggac
      481 gctatcgcgc gcgggcaatt cgagatcctg taccagccgc agctccgcct cgccgatcaa
      541 cgtgtcgacg ccgtcgaagc gctcttgcgt tggaagcacc cgcagcgcgg gctgttgagc
      601 cccaccgatt tcatctcggt cgccgaagag agcgggcaca tcatcccgct cggcctctgg
      661 gtcatcgagg aggtggcccg ccagctcaag cgctgggaaa gtgccggagt gcccgtgccg
      721 cgcgtcgcga tcaacgtctc cgcgctgcag ttccgtcagc cggactttca tgaagtcctt
      781 cgcaccgcat tggaaacgca ctcggtcgat cccagcctca tcgagctcga gctagcggag
      841 cattcgttga tgcaggacac gagcggcacg cgcgactcgc tgcgcgcatt gaagagtgtc
      901 ggcgtgcggc tcgcgatcga cgacttcggc gccggctatt cgtgcctcag ctatctgcga
      961 cagtttccgc tcgacgtgct gaagatcgac cgttcgttcg tctccgactt ggacacgagc
     1021 ttggacgctc aggctatttg cagcgcgatc ttgtccatcg cgcaccgcct gtcgctcgac
     1081 gccgtagcgg agggcatcga gacggagcgg cagctcgcgt ttctgacccg caacgattgc
     1141 ttgtacggcc agggatttta tttcagcgca cccgtcgagg ccgacaagat cgccgtgttg
     1201 atggcccaga gcggagcgca gtccacccga cgccgtcgag tgacgagccg acgaatcgcc
     1261 gcgaaagcgg gttgagaggt agcgcatgac cgacgccaat cgcccgcgcc cgacaacact
     1321 cgaagacgcg cttcgagcgc tggacgccgc cgaagagaag ctgcgcgctc aggtcgacga
     1381 taccggcatc cgccagctca gcgagaacgc gcagcgcagc gaggagcgct ttcgcatgct
     1441 cgccgaaggc gtgccgaatc acctgctgtt tctcgatcgc gagctgcgca tcacgttcgc
     1501 caacgacgtg ttcctcgagg ccgtcggctg gtcggcggac aaggccatgg gttgccacat
     1561 ttccgacatc atgggcgtcg agcgctatct cgaacggcag ccttactacg agcgcgcgct
     1621 tgccggcgag acggtcagct acgagtcgac gggcgcggcc ggcagcgagc acggctattt
     1681 tcgcttctcg tatcggccga gcttcgacga agccggcaag gtgcgcggca ttttttcaat
     1741 ggccacagac atctcggcgc ggcgcaagat cgagctggag ctcgaggcca agcaagccga
     1801 gctgctgcgc tcgaacaagg acctcgagca attcgcgtac gtcgcatcgc acgacttgaa
     1861 agcgccgctg cgcgccatcg atttgctcgt tcaatggatc gtcgaagggc ttgccggcta
     1921 cgacgccaac aacgtgcagg agaacctcgg gctactcggc aagaggacgc agcggctgaa
     1981 ccggctgctc gacgacctgc tcgcgtattc gcgcgccggc agaaaggtcg gcgcgcatcg
     2041 catcgtcgac gctcacgcgc tcgtgctcga cgtcattcag atgctgaact cgccggcgac
     2101 ggtgtcggta tcgatcgaag gcaagctgcc gacgttcaag acgcacccga cgccgctcga
     2161 gaccgtgctt cgcaacctca tcagcaacgc catcaagcat caccccggcc cgcagggccg
     2221 cgtcgtcgtg tcctgccaag agcagtcgga ccagtacgtc tttgccgtcg aagacgacgg
     2281 cgaaggcata ccgccgcaat acgcggagcg tgtgttcgag atgtttcaaa cgttgaagcc
     2341 gcgcgatcaa gtcgaaggca gcggcatggg gctcgcgatc gtgaaccgca tcgtgcagtg
     2401 gcagagcggc cgcgtctggt tcgagccggc cccgtcaggc cgcggcgccg tgttcaagtt
     2461 ccagtggaaa aagaatcagc cgttggccgc caaagtggag acgaaggcat gggaagcagc
     2521 aaggtagtca cgttccttct cgtcgaggac gacgagatcg acgtcaaagc cctcaaatgg
     2581 gcgttcgaca agctgaagat tgccaatccg ttgaagatcg cgcgcgacgg cgtcgaggcc
     2641 tgggagatgc tccaggagct gccgcgtccg tatctgatca tctcggacat caacatgccg
     2701 cgcatgaatg gcatcgagct gctgcgcaag atccggcaga gcgagcactg ccgcgactcc
     2761 atcgtgttca tgctgacgac gtccaacgac gagcaggaca agatcgacgc ttacgacctc
     2821 aacgtggccg gctacatgct gaaatccgac atgggcacga gcttcacacg ggcgatcagc
     2881 ctgatcgaca actactggaa ggtcgtcgag ttccccgagc acgtctgacc gccgagcgac
     2941 ggcctagatc aagcccgcgc gcttgaccgc gtgataacga ttgacgagct cgacaccgag
     3001 ccgctcgggc tcggcgtcga ccatcagagc gtcgcgtgcc gcgagccgtc gaaacgcatc
     3061 gcggcgcgct tgctcgtaga ggtgcgcact cgcgacgtcg agcgcggcgt tgcccgacgt
     3121 cagcggctgc gcgatcagct cgccgacgat gcgctcgcgc acgctggcaa gcagcacgag
     3181 atgccgctgg cgcaatagct tcagcgcgag gcccagctcg ctgccgtcct cgtcgcggaa
     3241 gttcgtgatc acgatcacga gcgcgcgttt gttctgccgc cgcagcaagt ccttcgccgc
     3301 cgcgacgtag tccgaatggc tcggcgtcgg ctgcagcccg taaagctcgc ccatcagtgc
     3361 attcaagaca tgcgcgcctt tgcgtggcgc gaatagccgc tcgccgccgg gcggcgtgcc
     3421 gaacgtcaag gcgccgacgg cgtcgccctg cgcaagcgcc acgtacgaca gcaacatcac
     3481 ggcgttcagc acttgatcga agtgcgagct gcc
//
LOCUS       DATLEE010000151         6586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_905429, whole genome shotgun sequence.
ACCESSION   DATLEE010000151 DATLEE010000000
VERSION     DATLEE010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905429"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VM692_03235"
     CDS             <1..426
                     /locus_tag="VM692_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUQ51208.1"
                     /translation="ALRNAVSGHIRGGSTLTPRHRETVIMRIGVLCRSEYEWAAHMRA
                     GKGAGMTDADVERVARGPDAAGDPLEKALLRATDELYRDDRVSDATWAELAAKLDTKQ
                     LLDVLIAVGGYRTASMTINSAGVQLDANMADFRFPAFLR"
     gene            complement(514..1272)
                     /locus_tag="VM692_03240"
     CDS             complement(514..1272)
                     /locus_tag="VM692_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51209.1"
                     /translation="MKRELLMAAAAAGMWLAASAAAQIPTAGEHNADLSGVWTNVNQP
                     GTAEWAIYTFSKDLPEMTAWGKARFDAAKPQRGPRGVPVAETDDLVYKCYPPGTPRIY
                     IHPFPMEIIQLPGRVLMIFEYDHLVRQIYTDGREHRTDLPASWMGDSIGHWEGKTLVV
                     ETNNFNDKTWIDRIAVPHSEEMVLTERFQLNEQGQLEIDMRIDDPKALAKPWEFSRWY
                     RKTDWTIEELVCEDNATYEEFENQLLEFDDERAE"
     gene            complement(1283..1672)
                     /locus_tag="VM692_03245"
     CDS             complement(1283..1672)
                     /locus_tag="VM692_03245"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51210.1"
                     /translation="MSSRVGFKLVLALGLLAGSQAIAHHGAGVFEPEKKVTVAGTVTD
                     FQFVNPHVLVYIDVKGEGGKAVHWGGELTSPNRLARDARGVKWHKEILKPGDSITMTG
                     HPARNGAPMMDILKIVDGQGTVLIDDE"
     gene            complement(1708..2721)
                     /locus_tag="VM692_03250"
     CDS             complement(1708..2721)
                     /locus_tag="VM692_03250"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HUQ51211.1"
                     /translation="MRPCFHLVTSFAAAIALLVPMSLAAQTETPAGAGVIIRHDARFD
                     SLVPAGATIEKVADGFVFLEGPVWRKSDARLFYSDLRGNAIYQWTAADGVSDLSKPFF
                     VGEGTGLRGVGPNGIALDSQGRLLACVYGSRSVVRLEKDGTRTTLAERFEGKRLNSPN
                     DLVVARDGTVYFTDPSFGLEGTDKSPLRELDFNGVYRIKPNGQLDVLARDLERPNGLV
                     LSPDEKILYVANSGGGAVTGWLAYDLSSNGLANQRVLLDVTGVQGEGGADGMKVDSAG
                     NVYATGPGGVWVIAPDGTHLGTIRPPEALTNVGWGDDGHTLFITGRTALYRIRLTARG
                     AVQ"
     gene            complement(2836..3336)
                     /locus_tag="VM692_03255"
     CDS             complement(2836..3336)
                     /locus_tag="VM692_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ51212.1"
                     /translation="MSTIRLCKPEERSAMLAIVNAAATRYHGVIPADCWHEPYMSIDQ
                     LERDIAAGATFWGYEDDAGNLLGVMGMQAVRDVELIRHAYVRPDRQGCGFGGALLKFL
                     ETLSRRQILIGTWADAEWAIRFYERHGYELVPRAKAAELLRTYWTVSARQIDTSVVLA
                     KPHVSR"
     gene            3430..4500
                     /locus_tag="VM692_03260"
     CDS             3430..4500
                     /locus_tag="VM692_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUQ51213.1"
                     /translation="MSARFLFAAVQAGAERALKNEVAREQPQLKFAFSRPGFVTFRVP
                     DDGAAGRAPRLDSVFARTWGHSLGKVAGTDDAQLARDAWRLVADALPSGEAQQIAHVH
                     VWQRERALAGDEGYDSSADDLARAVCEVLLAQRPGSIEPRPFVNASAAAGELVLDCVL
                     VERGEWWVGWHRAESPETRWPGGVPPIAVPPRLISRAYLKLVEALEWSQLPLKAGDRC
                     VEIGSSPGGSCLALLERGLVVTGIDPAEMDPAVLAQPNFTHLRARAKDVKRSVFRDCR
                     WLVMDANVAPNYTLDTLDGLLTQAGVRPEGLVLTLKLTDSKLAEKLPAVAERIRRHGY
                     RRVRMRQLAFNRQEICAVVTDG"
     gene            4574..5668
                     /locus_tag="VM692_03265"
     CDS             4574..5668
                     /locus_tag="VM692_03265"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HUQ51214.1"
                     /translation="MLGTLCGAPALAQAPSAALPAAATSAMQMTQAARALLASLSDSE
                     RARSVLALDAGERSSWSNVPYQAHHRPGLKLGGLDRARMLLVHELLRASLSSQGYQKV
                     AGIMRLDDINRAQQVARLSPDATAYQKAQAESFGSDNYFILFFGDPRRDARWGWLIQG
                     NHLAVSFTVADGRTAFLPMFVGATPLAVEEDVETGWSALAQEVARGVELVTALSESQR
                     KIAISPAEVPDDVLNGVGLKHRFTPAEGLRAADMTAEQRRLLRSLVEEYVDNADFDAA
                     DEQLAAIEKAGWDNLAFTWRGAVGDLAEPFYYRVQGPRIVIELRNTANHVHTVTRDPA
                     NDYGEAWLGISYRETITAAERSAAARRAAE"
     gene            5692..>6586
                     /locus_tag="VM692_03270"
     CDS             5692..>6586
                     /locus_tag="VM692_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ51215.1"
                     /translation="MDAVISGRRHFLATAMRLAAASAVGGGVLRSPRWGTAGAQSTAL
                     TETPLRENLSLITAGVSNVVVLRTAGSAALIDSGPPEHAEELARLVRGKLGLLAVELL
                     FNTHWHPTHTGGNEALRSGETDIIAHEITRLWMSTEYYVDWEDKTYEPRAAAALPTRT
                     FYSTDPQPLTVEIGDEEIQYGHLPEAHTDGDVYVWLKNRNVLAAGGAVTVGEYPVIDY
                     STGGWIGGLVDATKKLLDLTNPDTLIVPANGPAQPRSHLQAQLEMLTVVRERIENLMR
                     KGRSIEEMLAADVTKEFDAKWG"
BASE COUNT         1102 a         2215 c         2164 g         1105 t
ORIGIN      
        1 gccctgcgca acgccgtcag cggccacatt cgcggcggct ccacattgac gccgcgccac
       61 cgcgagaccg tgatcatgcg gatcggcgtg ctgtgccgct cggagtacga atgggcggcg
      121 catatgcgcg ccgggaaggg cgccggcatg acggatgccg acgtcgagcg tgtcgctcgc
      181 gggcccgatg cggccggcga cccgctcgag aaagcgctgc tgcgcgcgac cgacgagctc
      241 tatcgcgacg atcgagtatc cgatgcaact tgggccgagc tcgcggcaaa gctcgacacg
      301 aagcagctat tggacgtgct gatcgccgtc ggcggctatc gcacggcgtc gatgacgatc
      361 aacagcgccg gcgtgcagct cgacgccaac atggcggact tccgcttccc ggctttcctg
      421 cgctgaagcg cgctatgccg gccgcagccg cgcttgcggc ccgccgccga gccgccgagc
      481 aggccgagat ccacgagggc ggccgatgac gacctactcg gcgcgttcgt cgtcgaactc
      541 gagcagctgg ttctcgaact cttcgtaggt cgcgttgtcc tcgcagacca gctcttcgat
      601 cgtccaatcg gtcttgcgat accagcgcga gaactcccac ggcttcgcga gcgctttcgg
      661 gtcgtcgatg cgcatgtcga tctcgagctg accctgctcg ttcaactgaa agcgctcggt
      721 caacaccatc tcttcgctgt gcggcacggc gatgcggtcg atccacgtct tgtcgttgaa
      781 attgttcgtc tcgacgacga gcgtcttgcc ttcccagtgc ccgatcgagt cgcccatcca
      841 agaggccggc aagtcggtgc gatgctcgcg gccgtccgtg taaatctgcc gcacgaggtg
      901 gtcgtactcg aagatcatca gcacgcggcc cggcagctgg atgatctcca tcgggaacgg
      961 atggatgtag atgcgcggcg tgccgggcgg ataacacttg tagacgagat cgtcggtctc
     1021 ggcgacgggc acgccacgcg ggccgcgttg cggcttcgcg gcgtcgaacc tcgccttgcc
     1081 ccacgccgtc atctcgggca gatccttgct gaacgtgtag atcgcccact ccgcggtgcc
     1141 gggttggttg acgttggtcc agacgccgct caagtcggcg ttgtgctcgc ctgccgtcgg
     1201 gatctgcgcc gcagccgacg cagccagcca catgccggca gcggcggccg ccatcaatag
     1261 ttcacgcttc atctctcccc cgctactcgt cgtcgatcaa cacggtgcct tgaccgtcga
     1321 cgatctttag aatgtccatc atcggcgcgc cgttgcgcgc gggatggccc gtcatcgtga
     1381 tgctgtcgcc gggtttcaag atctctttgt gccacttgac gccgcgcgcg tcgcgcgcga
     1441 gccggttcgg gctcgtgagc tcgccgcccc agtgcacggc cttgccgccc tcgcctttga
     1501 cgtcgatgta gacgagcacg tgcggattca cgaactgaaa gtccgtgacg gtgcccgcga
     1561 ccgtgacctt cttttcgggc tcgaagacgc cggcgccgtg atgtgcaatc gcttggcttc
     1621 ctgccagcaa tccgagcgcc agcaccaact tgaaaccgac tctgctgctc atgaacgttt
     1681 ctcccggttc cgcgctgcgc aacgccgtca ctgaacggcg ccgcgggcgg tcaatcttat
     1741 gcgatacaac gccgtgcgcc ccgtgatgaa caacgtgtgc ccgtcgtcgc cccagccgac
     1801 gttcgtcaac gcttcgggcg gtcggatcgt gccgaggtgc gtgccatcgg gcgcgatgac
     1861 ccagacaccg ccgggccccg tcgcgtagac gttgccggcg gaatcgacct tcatgccatc
     1921 ggcgccgccc tcgccttgca cgcccgtgac gtcgagcagc acgcgttggt tggctagccc
     1981 gttcgagctc aagtcgtacg cgagccaacc cgtgacggca ccgccgccgg agttcgcgac
     2041 gtagaggatc ttctcgtcgg gcgacagcac gaggccgttc ggccgctcga gatcgcgcgc
     2101 caggacgtcg agctgcccgt tcggcttgat gcggtagacg ccgttgaagt cgagctcgcg
     2161 cagcggcgac ttgtcggtgc cttccaagcc gaacgacggg tccgtgaagt acacggtgcc
     2221 gtcgcgcgcg acgacgagat cgttggggct gttgagccgc ttgccctcga agcgctccgc
     2281 gagcgtcgtg cgcgtgccgt ccttctcgag ccgaacgacc gagcggctgc cgtagacgca
     2341 cgcgagtaag cgcccttgcg aatcgagcgc gatgccgttc ggaccgacgc cgcgcagccc
     2401 cgtgccttcg ccgacgaaga acggcttcga caggtcgctg acgccgtcgg ccgccgtcca
     2461 ttgatagatc gcgttgccgc gcaagtccga atagaagaga cgcgcatcgg acttgcgcca
     2521 gacagggccc tcgagaaaca cgaagccgtc ggcgactttc tcgatcgtcg cgcccgccgg
     2581 cacgagcgaa tcgaagcgcg cgtcgtggcg gatgatcact ccggcgccgg ccggagtttc
     2641 cgtttgcgcc gcgagggaca tcggcacgag cagcgcgatg gccgccgcga agctcgtcac
     2701 cagatggaag cagggtcgca tgccgagcac ctcgcgcgat catctcctcg tatgctagcg
     2761 caacagcgtt cgcgtcgccc aaaccgatgc gtttgcccgt gcggtgcagc gcctagcaac
     2821 cgaccgcctc acggctcatc gcgacacgtg cggctttgcg agcactacgg acgtgtcgat
     2881 ttggcgcgcc gacacggtcc agtaggttcg caacaactcg gcggccttcg cgcgcggcac
     2941 gagctcgtag ccatgtcgct cgtagaaacg tattgcccac tcagcgtcgg cccacgtgcc
     3001 tatcaagatc tgccgccgac tcaacgtctc gaggaacttg agtagcgcgc cgccgaatcc
     3061 gcagccttgt cgatcgggcc gtacataggc gtgacgaatc agttcgacgt cacgcaccgc
     3121 ctgcatgccc atgacgccga gcagatttcc cgcatcgtcc tcgtatcccc aaaatgtcgc
     3181 gccagcggcg atgtcacgct cgagctgatc aatggacatg taaggctcat gccagcaatc
     3241 agcgggaatg acgccgtgat agcgagtggc cgccgcgttg acgatcgcga gcatcgcgga
     3301 acgttcttcg ggcttgcaaa ggcggatcgt gctcacgcca acgaaactct gttacccgct
     3361 ccgttgcggg tgctacgagt atactgagcg accgcaagcc tgccgttgac gatcatcttc
     3421 tcgcacgcca tgtcggcccg cttcttattt gccgctgtcc aagcgggcgc cgaacgagca
     3481 ctgaagaacg aagtcgcgcg cgagcagccg cagctgaagt tcgcattctc gaggccgggg
     3541 ttcgtcacgt tccgcgtgcc cgacgacggc gcggccggtc gcgcgccgag gctcgattcc
     3601 gtattcgcgc gcacgtgggg ccactcgctc ggcaaggtcg cgggcactga cgacgcgcag
     3661 ctcgcacgcg acgcgtggcg gctcgttgcg gacgcattgc cgagcggcga agcgcagcag
     3721 atcgcgcacg tgcacgtctg gcagcgcgag cgcgcgttgg ccggcgacga gggctacgac
     3781 tcgtcggccg acgaccttgc ccgcgccgtc tgcgaagtgc tgctcgcgca gcgcccgggc
     3841 tcaatcgagc cgcggccctt cgtcaacgcc tcggccgctg ccggcgagct cgtgctcgac
     3901 tgcgtgctcg tcgagcgcgg cgagtggtgg gtcggctggc atcgagccga gtcgcccgag
     3961 acgcgttggc cgggaggcgt gccgccgatc gcggtaccgc cgcgcctgat ctcgcgcgcg
     4021 tatctgaagc tcgtcgaggc gctcgaatgg tcgcagttgc cgctgaaggc cggcgaccgc
     4081 tgcgtcgaga tcggcagctc gccgggtggt tcgtgcctcg cgctgctcga gcgcgggctc
     4141 gtcgtgaccg gcatcgaccc ggccgagatg gatccggccg tgctcgcgca gccgaatttc
     4201 acgcatttgc gtgcacgcgc caaagacgtc aaacgcagcg tgttccgcga ttgccgctgg
     4261 ctcgtcatgg acgcgaacgt cgcgccgaac tacacgctcg acacgctcga cgggcttttg
     4321 acccaagccg gtgtccggcc ggaaggcctc gtgctgacgt tgaagctcac ggattccaag
     4381 ctcgccgaga agctgccggc cgtcgccgag cgcatacgcc ggcatggcta ccgccgtgtg
     4441 cgcatgcggc agctcgcgtt caatcgccaa gaaatctgcg ccgtcgtcac cgacggctga
     4501 acagccgcta tcatggctcg caacgggcgg gggttgcgaa atgagcatcc gacgacagct
     4561 gtcatgcttg attgtgctag gcacgttgtg cggcgcgccg gcgctcgctc aagcgccgag
     4621 cgcagccctg ccggcggcgg ccacgagcgc aatgcagatg acgcaggccg cgcgcgcctt
     4681 gctcgcgtcg ctcagcgaca gcgagcgcgc gcggagcgtg ctcgcgctcg atgccgggga
     4741 acgctcgagt tggtcgaacg tgccgtacca ggcgcaccat cggcccgggc tcaagctcgg
     4801 cggtctcgac cgcgcgcgca tgctgctcgt gcacgagctg ctgcgagcgt cactgtcgag
     4861 ccagggctat cagaaagtcg ccggcatcat gcgcttggac gacatcaatc gcgcgcagca
     4921 agtcgcgcgg ctctcgcccg acgcaacggc ctatcagaaa gcacaggccg agagcttcgg
     4981 ctccgacaac tacttcatcc tgttcttcgg cgatccgcgg cgcgatgcgc gctggggttg
     5041 gctcattcaa gggaaccatc tcgccgtcag cttcacggtc gccgacggca ggacggcatt
     5101 cctgccgatg ttcgtcggcg cgacaccgct cgccgtcgaa gaggacgtcg agacgggctg
     5161 gtcggcgttg gctcaagaag tagcaagagg cgttgaactc gtgacggcgc tgtccgagag
     5221 tcagcgcaag atcgcgatct cacccgctga agtaccggat gacgtgttga acggcgtcgg
     5281 cttaaagcac cgcttcacgc cggccgaagg cttgcgagcg gcggacatga cggccgagca
     5341 acgccgctta ctacggtcgc tcgtcgagga atatgtcgac aacgcggact tcgacgcggc
     5401 cgacgagcag cttgcggcga tcgagaaggc cggctgggac aacctcgcgt tcacgtggcg
     5461 cggcgcggtc ggcgatctcg ccgagccgtt ctactaccgc gtgcaggggc cgcgcatcgt
     5521 catcgagctg cgcaacacgg cgaatcacgt gcacacggtc acgcgcgacc cggcgaacga
     5581 ttatggtgaa gcgtggctcg gcatctcgta ccgagagacg atcacggccg ccgagcgctc
     5641 ggccgcagcg cgtcgtgcag ccgagtagct gcactcatct aatataggag catggacgca
     5701 gtgatttcgg gccggcgcca cttcttggca accgcgatgc gactcgcggc ggcatcggcc
     5761 gtcggcggcg gcgtgctgcg tagtccccga tgggggacgg ccggcgcgca aagcacggcg
     5821 ctgacggaaa ctccgctgcg cgagaacttg agcctgatca cggccggcgt cagcaacgtc
     5881 gtcgtgctgc gcacggccgg gtcggccgcg ctgatcgaca gcggcccgcc cgagcacgcc
     5941 gaggagctcg cgcggctcgt gcgcggcaag ctcggtctgc tcgccgtcga gctcttgttc
     6001 aacacgcact ggcatcccac gcacacgggt ggcaacgagg cactgcgctc cggcgaaacc
     6061 gacatcatcg cgcacgagat cacgcggctc tggatgagca cggagtacta cgtcgactgg
     6121 gaagacaaga cttacgagcc gcgcgctgcg gccgcgttgc cgacgcggac gttctattcg
     6181 accgatccgc aaccgttgac cgtcgagatc ggcgacgagg agatccagta cggccacttg
     6241 ccggaagcgc acacggacgg cgacgtctac gtctggctca agaatcgcaa cgtgctcgcg
     6301 gccggcggcg ccgtgacggt cggcgagtat cccgtcatcg actactcgac gggtggctgg
     6361 atcggcggcc tcgtcgatgc gacgaagaag ctgctcgacc tcacgaaccc cgacacgctg
     6421 atcgtgcctg cgaacggtcc cgcgcagccg cggtctcacc tgcaggcgca gctcgagatg
     6481 ttgaccgtcg tccgcgagcg aatcgagaac ctgatgcgta agggccgcag catcgaagag
     6541 atgctcgcgg cggacgtgac caaggaattc gacgccaagt ggggca
//
LOCUS       DATLEE010000152         2932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_162533, whole genome shotgun sequence.
ACCESSION   DATLEE010000152 DATLEE010000000
VERSION     DATLEE010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162533"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..517)
                     /locus_tag="VM692_03275"
     CDS             complement(254..517)
                     /locus_tag="VM692_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51216.1"
                     /translation="MTERIVELNSVDYEVTSASMGHGRNGAVFRCTLRPVTDAVLEEL
                     ELAAQSDGMVRLVFPKEPLLLERIDVQQVEPGCVRLAGRVTSK"
     gene            complement(664..1263)
                     /locus_tag="VM692_03280"
     CDS             complement(664..1263)
                     /locus_tag="VM692_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51217.1"
                     /translation="MFTNNRIVISLMIACAMVALALAATMDALAAAPRRVADTFTATT
                     ANMTPAGLSLRMQILEWQEAPARMEVVAALAAGGDTAAPLAKLPTVGYIWPAASPVGY
                     SVKYAYREPTAGGGERITLVTDKRLGSYDYKKWSVPSPAKELSYSVVVLDVSASGTGT
                     GNLSLGAAVMLDEAAGTVALMGPAGASLLTNVKRETTAH"
     gene            complement(1253..1756)
                     /locus_tag="VM692_03285"
     CDS             complement(1253..1756)
                     /locus_tag="VM692_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51218.1"
                     /translation="MKSEPRLINTRHRCVIAALLATAAVHPVAGNEHDKTYTWSAELV
                     ALDEQARTLTVQSRLVSDSAVDFDSFDSGDRLMLTWSGLNTAAGVRRLTEGAAAEADE
                     LTLPVEFVSPELDGRYVRFKVAVPSDDLAKLKAVQPGQYVTATSPRRASRWEEAVVDV
                     RPYNDVH"
     gene            1938..>2932
                     /locus_tag="VM692_03290"
     CDS             1938..>2932
                     /locus_tag="VM692_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51219.1"
                     /translation="MGLAVLGLFAMPCAAQTSVGRGAPDPVAAAVIVGSEGKGLFVGA
                     GIPEGNMPIFAARDGAVPAGVEPLPHDIFTTTDFYKDRELWSDPRYYRCNSAVGLEQI
                     WGAYEVPLIGDDPPRTAAWGFCDRDYPRKEIVSPYGFATAKAHYEALLREARAKGGPT
                     VYTQATLPDWNGRYRRQRAKTASWYYGAVLSIPTYLTLLTPEYQKRFVQQMYHYSGSN
                     AAQWPGSYCWPEGFMRRLSQYGGGEMNLVMTPDLILDMRNAAKTLITQIHIGRSFNEE
                     GVVPRLGPDVPQWFGETIGFWDGEALITWTSNIQGWISHGGFEFSNKLQSIEIYTP"
BASE COUNT          511 a          941 c          969 g          511 t
ORIGIN      
        1 acccagacga tgagaacgta aatgagcatg gcaagcgcgt tcaaaagcaa aggaaggagg
       61 cgggtcgcgg tcccgcgagt ctgacccatg gctgtcccct tttagtaatc gtgtcgtcct
      121 tgagggtcgg gccggagtat acccgcgagc gggcacaagt cgaggcgact tacgtcgcgc
      181 attcgcaggc gaatggcggc taattcccgt cgtgcaggga atgaccggag gatcggggcc
      241 ggcgcccgat cggctactta gaggtcacgc gcccggcgag ccgcacgcag cccggctcga
      301 cctgctgaac gtcgatgcgt tcgagcaaca gcggttcttt cgggaacacg agccggacca
      361 tgccgtcgga ttgtgccgcc agctcgagct cttcgagcac tgcgtcggtg acgggacgaa
      421 gagtgcagcg aaagaccgcg ccgtttcgcc catggcccat cgacgccgag gtgacttcgt
      481 agtcgacact gttgagctcg acgatccttt ccgtcatgga actctcccgc acggcattct
      541 actagccaac acggatgagt cgcgcggccg gttccatcgg acgcggatcg agcgcgacgc
      601 cggcgcggac ccacgatctg cgaaaagagc agggcggcgg ctaacgccgc cctgcacgga
      661 cgtttagtgc gccgttgtct cgcgcttgac gttcgtcagc agcgacgctc cggcggggcc
      721 catcagtgcg acggtaccgg ccgcctcatc gagcatgacc gccgcgccca gcgacaagtt
      781 gcccgtaccc gttccggatg cgctgacgtc gagcacgacg acggagtacg acagctcttt
      841 cgcagggctc ggtaccgacc acttcttgta gtcgtagctg ccgagccgct tgtccgtcac
      901 gagcgtgatg cgctcgccgc cgccggccgt cggttcgcgg tacgcatatt tcaccgagta
      961 ccccacgggg ctcgcggcgg gccagatgta gccgaccgtg ggcagcttcg cgagcggcgc
     1021 cgccgtgtcg ccgccggcgg ccagcgccgc tacgacttcc atgcgcgcgg gcgcttcttg
     1081 ccactcgaga atctgcatgc gaagactcaa gccggccggc gtcatgttcg ccgtcgtggc
     1141 cgtgaacgta tcagcgacgc ggcgaggtgc tgcggcgagg gcatccatcg tcgcggccag
     1201 ggccagggcg accatggcgc aggcgatcat caggctgatg acaatacggt tgttagtgaa
     1261 catcgttata cggtcgcacg tcgacgaccg cctcctccca acgtgaagcg cgccgcggcg
     1321 acgtcgccgt gacgtattga cccggttgca cggccttgag cttcgcgaga tcgtcgctcg
     1381 gcacggctac cttgaaacga acataacgac cgtcgagctc gggcgagacg aactcgaccg
     1441 gcaaggtcag ctcatcggct tcggccgctg cgccctcggt caggcggcga acgccggcgg
     1501 ccgtgttgag acccgaccac gtcagcatga gccgatcgcc gctgtcgaac gagtcgaaat
     1561 cgacggcgct gtcgctgacg agccgcgatt gcacggtcag cgtgcgtgct tgctcgtcca
     1621 aagcgacgag ctcggccgac cacgtgtaag tcttatcgtg ctcgttgccg gcaacagggt
     1681 ggactgctgc cgtcgcgagc agtgccgcga tgacgcaacg atgccgcgtg ttgattagcc
     1741 ttggttcgct cttcatggtg cctcctcact gttgcgagcg ttggttcaac ggccgaaacg
     1801 tatgggcacg acttttagcg tttcaagtcc ccccctgcgc gactggacag cgagccggcc
     1861 aaacgttgaa attgcgtcgg tcgccgggga tacagcgcat gacggtagct cgattgaggg
     1921 ttggcggaaa ggcctgcatg gggctcgccg ttttgggcct gttcgcgatg ccctgcgcgg
     1981 cgcagacgag cgtgggccgc ggtgcccccg acccggtcgc agccgccgtc atcgtcggct
     2041 cggaaggcaa ggggctgttc gtcggcgcgg gcattcccga gggcaacatg ccgatcttcg
     2101 ccgcgcgcga cggtgctgtg ccggccggcg tcgagccgtt gccgcacgac atcttcacca
     2161 cgaccgactt ctacaaggac cgcgagctct ggtcggatcc gcgctactac cgctgcaact
     2221 cggccgtcgg cctcgagcag atctggggtg cgtacgaagt cccactgatc ggcgacgatc
     2281 cgccgcgcac ggccgcatgg ggcttttgcg accgcgatta tccgcgcaag gaaatcgtga
     2341 gcccgtacgg ctttgcgacg gcgaaggcgc actacgaggc gcttctaagg gaagcgcgcg
     2401 cgaagggcgg cccgacggtc tatacgcaag ccaccttgcc cgattggaac ggccgctatc
     2461 ggcgccagcg cgcgaagacg gcgtcctggt actacggcgc ggtgctgtct attccgacct
     2521 atttgacgct gctgacgccg gagtatcaga agcgcttcgt gcagcagatg tatcactact
     2581 ccggcagcaa cgcggcgcag tggcccggtt cgtactgctg gcccgaggga ttcatgcggc
     2641 gcctctcgca atacggcggc ggcgagatga acctcgtgat gacgccggac ttgatcctcg
     2701 acatgcgcaa cgccgcgaag actctgatca cgcagatcca catcggccgc agcttcaacg
     2761 aggagggcgt cgtgccgcgc ctcgggcccg acgttccgca gtggttcggc gagacgatcg
     2821 gtttttggga cggcgaagcg ttgatcacct ggacgtcgaa catccagggg tggatctcgc
     2881 acggcggctt cgagttcagc aacaagctgc agtcgatcga gatctacacg cc
//
LOCUS       DATLEE010000153         5108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_163151, whole genome shotgun sequence.
ACCESSION   DATLEE010000153 DATLEE010000000
VERSION     DATLEE010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163151"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VM692_03295"
     CDS             <1..419
                     /locus_tag="VM692_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51220.1"
                     /translation="ESVGFWNGNQLVVWTANVQSWTLSHSMFEYSDKLEVVETFTPAE
                     NGGLVVEATFYDPESFVRPLHIVTPWNRTGGIDSADKRYTYVECRVQSTIVNGPDGRP
                     TQLTFGDDGYIDYFGRPWAQNWEEHFEQGWADEPAQ"
     gene            complement(436..672)
                     /locus_tag="VM692_03300"
     CDS             complement(436..672)
                     /locus_tag="VM692_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51221.1"
                     /translation="MTDAELANVWSTLGPNARRRARIETRVFEWLDASETSIAAEWLA
                     LLKIEPLTGLAYASLGALSLVLLSPLGWIVAALL"
     gene            complement(669..1334)
                     /locus_tag="VM692_03305"
     CDS             complement(669..1334)
                     /locus_tag="VM692_03305"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUQ51222.1"
                     /translation="MILTATMATLPAAWPLSSLLNALSVLFGADAEALDEDAVLRLVA
                     AARSGDRAARQRLYRQHVQRVFRTVRGILRSDAEAEDVTQDAMLTALTSLERYSPRSG
                     TRFVAWLTTIAVNTARRRFRRRRPELTATGELPEVAGTDAELGDDLDLARQRAALLRA
                     LAELEPREREIVNLRYGAELNAAEIATQLGIEAANVRKILERARVRLGDRIEALARTG
                     ATA"
     gene            complement(1331..2161)
                     /locus_tag="VM692_03310"
     CDS             complement(1331..2161)
                     /locus_tag="VM692_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51223.1"
                     /translation="MSIFAMIKEALRIARTHKSLWIFGFLVGFGGNVNFGGRGEQVGL
                     PVPAAPPELGTIVSIGLVVVAVLVVAVILRFLSTGALIEGVRRALSNDSMTVSEGFRE
                     GWAHWGVLFRIGLLYLLFNAISAIVLFGGCMLVWRVFGSPAVFVAGGIAALIGIPWLL
                     TLYMWQSFAERIAVLEDRRALDAIYKARLFLHGRLRLGLKLLAAGFLGVFFVFFVGAA
                     LIAPIAAIVIASAAAWGVVGAVGLGLLTLLPAAFVAFAVIGIVTSSVWTIGYLQQVEQ
                     "
     gene            complement(2303..2785)
                     /locus_tag="VM692_03315"
     CDS             complement(2303..2785)
                     /locus_tag="VM692_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51224.1"
                     /translation="MTTQSFRQILETLEAHGVEYVVVGGVAAVLQGAPVTTFDIDTLV
                     KVDPENSRRLLNALAELDARFREHDSLRPSAADLAAGGHMLLLTRSGPLDVLGFIGGG
                     KRYEDIADRTEYVRIGNLSIRVLTLQALLEEKRALGRDKDLPVVRILEALVARRKRDS
                     "
     gene            complement(3430..3891)
                     /locus_tag="VM692_03320"
     CDS             complement(3430..3891)
                     /locus_tag="VM692_03320"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51225.1"
                     /translation="MRKTLVAAAAALLASGAALAHHSFAMFDSSQHKLIEGEVAEWNY
                     NNPHSWLIVNAPGADGKMTTWSFEGAAIVHAARQGVNGKSYTKGEHVRIVMHPLRDGR
                     NAGALCFVVKEDGTFTRPNDGICNSDNMYARWQEKGWLASGHHLDTHPTAD"
     gene            complement(3893..4915)
                     /locus_tag="VM692_03325"
     CDS             complement(3893..4915)
                     /locus_tag="VM692_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51226.1"
                     /translation="MRSSRTLVALAVAAALTAALSGTHAQNRQTGGTVEDQFVLPVPE
                     VEKPPREFKTAEEHYNYLLERAHGGTKHTMQTIPVWDGLWGSGNNTMPSIFLENGTLA
                     NAWRPGQTKVKEGVLTPPYEQAFRERRAEIDKYGEQRYDRLTNCEYPGVPRWLWEPYI
                     KEFVNLPNQSWLMNDMMNETRRIYIGKEHVNIEQKHSALGDSIGFWDNDKLTIWTKWV
                     NPADYVRGMPLTSNQFEMVETWQARQNGTKRELVTQVTFYDPLALQKPQSAVYVHESR
                     PDLEEAGVRIRMWECTTSSNSYKDAEGNTQFYLPGDPQYKDPRGFTDFPDLPGQSLNP
                     IFDAEQ"
BASE COUNT          916 a         1662 c         1616 g          914 t
ORIGIN      
        1 gcgagagcgt cggcttctgg aacggcaatc agctcgtcgt ctggaccgcc aacgtgcaat
       61 cgtggacgtt gtcacactcg atgttcgagt acagcgataa gctcgaggtc gtcgagacgt
      121 tcacgcctgc cgagaacggc gggctcgtcg tcgaggcaac gttctatgat cccgagtcgt
      181 tcgtgcggcc gctgcacatc gtgacgccgt ggaatcgcac gggcggcatc gacagcgccg
      241 acaagcgtta cacgtacgtc gagtgccgcg tgcagagcac gatcgtcaac gggccggacg
      301 gccggccgac tcagctgacg ttcggcgacg acggctacat cgactacttc ggccgcccgt
      361 gggcgcaaaa ctgggaagag cacttcgagc aaggctgggc cgacgagccg gcgcaatagc
      421 gcggcagctg cgcgcctaca gcagcgcggc cacgatccag ccgagcggcg acaagagcac
      481 gagcgacaac gcgccgagcg aggcatacgc caagcccgtc agcggctcga tcttcaacag
      541 ggcgagccac tcggccgcga tcgaagtctc gcttgcgtcg agccactcga agacgcgcgt
      601 ctcgatgcgc gcgcggcggc gtgcgttggg gccaagcgtg gaccatacat tggcgagctc
      661 tgcgtccgtc atgctgtcgc tccggttctt gccagcgcct cgatgcggtc gccgaggcgc
      721 acgcgcgcgc gctcgagaat cttgcgcacg ttcgcggcct cgatgccgag ctgcgtcgcg
      781 atctccgcag cgttgagctc cgcgccgtaa cgcaggttta cgatctcccg ctcgcgcggc
      841 tcgagctcgg ccagcgctcg cagcagcgca gctcgctgcc gcgcgagatc gaggtcgtcg
      901 ccgagctcgg cgtccgtgcc cgcgacttcc ggcagctcgc cggtcgcggt cagctcgggg
      961 cgcctgcggc gaaagcgccg ccgcgccgta ttgacggcga tcgtcgtgag ccaagcgacg
     1021 aagcgcgtgc cgcttcgcgg cgaataacgc tcgagcgacg tcagcgctgt cagcatcgcg
     1081 tcctgcgtga cgtcctcggc ttctgcgtcg gagcggagga tgccgcgtac ggtacggaac
     1141 actcgctgca cgtgctggcg atacaggcgc tggcgcgccg cacgatcgcc gctgcgcgct
     1201 gcggcgacga gccttagcac tgcatcctca tcgagcgcct cggcgtctgc gccgaacaag
     1261 accgagagcg cgttcagcag cgaggaaaga ggccacgctg cgggcagcgt ggccatcgtc
     1321 gcagtcagaa tcattgctcc acctgctgca gataaccgat cgtccagacc gaggacgtca
     1381 cgatgccgat gacggcgaac gcgacgaagg ccgccggcag cagcgtcagt agaccgagcc
     1441 cgacggcacc gacgacgccc caggcggccg ctgacgcgat gacgatcgcg gcgatcggcg
     1501 cgatcagggc ggcaccgacg aaaaacacga agaacacacc gagaaacccc gcggcgagca
     1561 gcttcagccc gagccgcagc cggccgtgca ggaacagccg cgctttgtag attgcatcga
     1621 gcgcgcggcg gtcttcgagc acggctatgc gctcggcgaa cgactgccac atgtacaacg
     1681 tcagcagcca cggaataccg atcagcgccg cgatgccgcc ggcaacgaac acagccggtg
     1741 agccgaacac ccgccacacg agcatgcacc cgccgaacag cacgatggcg ctgatcgcgt
     1801 tgaacaagag gtacagcaag ccgattcgaa aaagcacgcc ccagtgcgcc cagccttcgc
     1861 gaaatccttc gctcacggtc atcgagtcgt tgctgagcgc gcgcctgacg ccttcgatca
     1921 gcgcgcccgt gctcaagaac ctcagaatga cggccacgac gagcacggct acgaccacga
     1981 gcccgatcga cacgatcgtc ccgagctccg gcggagcggc cggcacggga agcccaacct
     2041 gctcacctcg gccgccgaag ttgacgttac cgccgaagcc gacgaggaaa ccgaagatcc
     2101 ataacgactt gtgcgtgcgc gcgatccgta gcgcttcctt gatcatcgcg aagatactca
     2161 cagctctacc tcccaaggac ggcgacccgc cgccttctct agaggcataa gccgcttggg
     2221 gtggctagtg tgacatccgc gacccggccg ctagggatcc gccacgtggc ggattcggca
     2281 cgaccttgcc gcggcggcac ggtcatgaat cccgcttgcg acgcgcgacg agggcttcga
     2341 ggatgcgcac gactggcagg tccttgtccc tgccgagcgc cctcttctcc tcgagcaacg
     2401 cctgcaacgt caggactcgg atcgataggt tgccgatccg gacgtactcg gtgcgatcgg
     2461 ctatgtcctc gtagcgcttg ccgcccccga tgaatcccag cacatcgagc ggcccgctac
     2521 gcgtcagcag aagcatgtgc ccgccggctg cgaggtccgc ggcgctcggc cttagcgaat
     2581 cgtgctcgcg aaagcgtgca tcgagttccg cgagggcatt caggagacgg cggctgttct
     2641 ccggatcaac cttgacgagc gtgtcgatat cgaaggtcgt gacgggcgcg ccctgcagca
     2701 cggcggctac tccgccaacg acgacgtact ccacgccgtg ggcctcaagc gtctcgagaa
     2761 tttggcggaa gctttgagtc gtcatcgcgg cgcgccagat tgaaaaactc cacgcgtgat
     2821 tcgagctcgg cgagccgctc ggcgggcgtc aacgacagga accaatcgag caacgactca
     2881 tcattctcga cgactgttgc aacttgacga cgcgtatgca cgccatcatt ctagctccgt
     2941 catcgacatg ccgtcgccat gacgccattg atcaacaatg ttcggaaaga ggcagtggcg
     3001 acccctcacc gtctgacggc gtcgattttc gaacggctac gtaaacgtga tcgacgcaaa
     3061 atccaagaga cacatccgga ttgatacgtc gcaagacgta ctcgttcaac atcagcgact
     3121 acttggcgtt agctatgccg gccgtaaagg cagctttaga gcggtaagct gatacatggc
     3181 aacgcaccgt ggcgatctgc cgcccggcaa taagagcatg cgttgatgga ttttggattt
     3241 tggattttgg tacgaaatgg aacgcatctg tttgatcgaa tacctgcgat caaacgcgtt
     3301 gaggatcgac tctgtgccga gttcgataaa gagtcgtcgg cggtcgggca tgtgggcatg
     3361 tacggacgct ctcgcgctgg gacgcctaag caacgtcctc tcagttgact cgtgcgggca
     3421 ccgatgtcgt cagtcggcag tcgggtgcgt gtcgaggtga tgccccgagg caagccaccc
     3481 cttctcttgc cagcgcgcgt acatgttgtc cgagttgcag atgccgtcgt tcgggcgcgt
     3541 gaacgtgccg tcctctttga cgacgaaaca cagcgcgccg gcgttgcggc cgtcgcgcaa
     3601 ggggtgcatg acgatgcgga cgtgctcgcc tttcgtatag ctcttgccgt tcacgccttg
     3661 gcgcgcggca tgcacgatcg cggcgccttc gaagctccag gtcgtcatct tgccgtcggc
     3721 accgggcgcg ttgacgatga gccagctgtg ggggttgttg tagttccact ccgcgacttc
     3781 gccttcgatg agcttgtgct gcgacgaatc gaacatcgcg aacgagtgat gcgcgagcgc
     3841 ggcgcccgat gccagcaacg ccgccgcagc ggccactagt gtctttctca tctcattgct
     3901 ccgcatcgaa gatcgggttc aacgattggc ccggcagatc cggaaagtcc gtgaagccgc
     3961 gcgggtcttt gtactgcggg tcgccgggca aatagaactg cgtgttgcct tcggcatcct
     4021 tgtaggagtt gctgctcgtc gtgcactccc acatgcggat gcgcacgccg gcctcttcga
     4081 gatcgggccg cgactcgtgg acgtaaacgg ccgactgcgg tttctgcagc gcgagcggat
     4141 cgtagaacgt cacttgcgtg acgagctcgc gtttggtgcc gttctgccgc gcttgccacg
     4201 tctcgaccat ctcgaactga ttgctcgtca gcggcatgcc gcgcacgtag tcggccgggt
     4261 tcacccactt cgtccagatc gtcagcttgt cgttgtccca gaagccgatg ctgtcgccga
     4321 gcgccgagtg cttctgctcg atgttgacgt gctctttgcc gatgtagata cggcgcgtct
     4381 cgttcatcat gtcgttcatc agccacgact ggttcggcag gttgacgaac tctttgatgt
     4441 acggctccca gagccagcgc ggcacgccgg ggtactcgca gttcgtgagc cggtcgtagc
     4501 gctgctcacc gtacttgtcg atctcggcgc ggcgctcacg gaaggcctgc tcgtaaggcg
     4561 gcgtcagcac accctccttg accttcgtct gtccgggccg ccaggcattc gccagtgtgc
     4621 cgttctcgag gaagattgac ggcatcgtgt tgttgccgct gccccagagc ccgtcccaga
     4681 cggggatcgt ctgcatcgtg tgcttcgtgc cgccgtgcgc gcgctcgagc agatagttgt
     4741 agtgctcttc ggcggtcttg aactcgcgcg gcggcttctc gacctcgggc accggcaaga
     4801 cgaactggtc ctcgacggta ccgcccgtct gccggttctg cgcgtgggtg ccgctcagcg
     4861 ccgccgtcaa ggcggccgcg acggccaaag ccacgagagt tctgctgctg cgcatgacgc
     4921 accccctcgg atggttacga cggccgcttc ttgcggagct ccgcgagcgc cagccgctct
     4981 tccttgttcg gatcgttgac gacggcgacc ttctcgccga acaccattgt cggcagggtg
     5041 ttcggctcga acgtcggcca attcggcatg tcggcatggt tcggattgcc cgtgcgcgca
     5101 aacgcggc
//
LOCUS       DATLEE010000154         2699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3892278, whole genome shotgun sequence.
ACCESSION   DATLEE010000154 DATLEE010000000
VERSION     DATLEE010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3892278"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..848)
                     /gene="gloB"
                     /locus_tag="VM692_03330"
     CDS             complement(78..848)
                     /gene="gloB"
                     /locus_tag="VM692_03330"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011644318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HUQ51227.1"
                     /translation="MSALAIHMFPCLQDNYGYLLHDEDSGATAAVDTPDAAEIATQLD
                     AKGWRLTHILNTHHHADHAGGNLELKRRTGCTIVGPRADAARIPGIDVAVGEGDVVSL
                     GAHRAAVFDTPGHTRGHIVYHFEAAHAAFVGDTLFALGCGRLFEGTPGQMWSSLQKIL
                     QWPDDTRLYCAHEYTQSNARFASTVEPQNRALKERAETVARRRAAGHATVPSTLAEER
                     ATNPFLRPQSQDLRATIALANASDVDVFAKTRALKDAF"
     gene            936..>2699
                     /gene="thrS"
                     /locus_tag="VM692_03335"
     CDS             936..>2699
                     /gene="thrS"
                     /locus_tag="VM692_03335"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HUQ51228.1"
                     /translation="MPTITLPDGSQRSFPAPIDGSAVARSIGARLERDAVAVRIDGSL
                     KDLTLPIEHDARIEIVTRTSADGLELLRHDAAHIMAEAVKELYPETQVTIGPAIENGF
                     YYDFARSTPFTPEDLASIEKRMHEIVARDEKIVREVWDRDAAVKFFLEQGEAYKAEII
                     RSIPAGESITLYRQGNFIDLCRGPHLPSTGRLGAGFKLMKLAGAYWRGNSDNEMLQRI
                     YGTVWADAKELKAYLDRLEEAERRDHRKLGKQMDLFHFQDEAVGAVFWHTKGWQLFRT
                     LVEFMRSKQGAAGYREVNTPELMSRSLWEASGHWHTFGENMFTSKTAEEQTFVVKPMN
                     CPGHIQVFKSDLRSYRDLPYRLAEFGKVHRYEPSGALHGLMRVRAFTQDDAHVFCTEA
                     QITEEAGKVCQLLLDIYRSFGFDNVIMKFADRPPKRVGADEVWDRAEAALKSAMDSLG
                     LPYSLNKGEGAFYGPKIEFVLRDAIGRDWQCGTLQVDLNMPGRLGATYIGEDGNRHTP
                     VMLNRAIFGSLERFIGILLEHYAGSLPLWLAPVQVVVTTITSDADAYAHEVVAELARA
                     GLRAEADLRNEKINYKVREHSL"
BASE COUNT          494 a          880 c          865 g          460 t
ORIGIN      
        1 gggcctcatg atgcaaggcc gcacgcagct gcgaccgagc gcctgaggcg aaagcaccct
       61 gctccgttag aaacgcgtta gaacgcgtcc ttgagcgcgc gcgtcttcgc gaatacgtcg
      121 acgtcggagg cgttcgcgag cgcgatcgtc gcgcgcaggt cctggctttg cggccgcaag
      181 aacggattcg tcgcacgctc ctcggcgagc gtgctcggca cggtcgcatg gccggccgct
      241 cgccgccgcg cgacggtctc cgcacgttcc ttgagcgcgc gattctgcgg ctcgaccgtc
      301 gacgcgaatc gcgcattgct ctgcgtgtac tcgtgcgcgc aatagagccg cgtgtcgtcc
      361 ggccactgca aaatcttttg cagcgagctc cacatctgac ccggcgttcc ttcgaataat
      421 cggccgcagc ccagcgcgaa tagcgtgtcg ccgacgaacg cggcatgcgc cgcctcgaag
      481 tgatagacga tatggccgcg cgtgtgcccc ggcgtgtcga agacggcagc tcgatgcgct
      541 cccagtgaga cgacgtcgcc ttcgccaacc gcgacgtcga tgccggggat gcgcgcggcg
      601 tcggcacggg gccccacgat cgtgcaaccc gtccggcgct tgagctcgag attgccaccc
      661 gcgtgatcgg cgtgatggtg cgtattcaga atgtgagtca gccgccagcc cttcgcgtcg
      721 agctgcgtcg cgatctcggc ggcgtccgga gtgtcgacgg cggctgtggc acccgagtct
      781 tcgtcgtgca gcagatagcc gtagttatcc tgcaggcacg gaaacatgtg gatcgcgagc
      841 gcgctcatgg ccacttccca tcagagatac ggcgattatc gtacaatcgc caggccgcgc
      901 gctcgtggcg cgcttttcgt cccaagccca ctcgtatgcc aaccatcacc ctgcctgacg
      961 gatcacaacg ttcgttccca gcaccgatcg acggcagcgc cgtcgcgcgc agcatcgggg
     1021 cgcgcttgga gcgcgacgcg gttgcggttc gcatcgacgg ctcgctgaaa gacctcaccc
     1081 tgccgatcga gcatgacgcc cgcatcgaga tcgtgacgcg cacgtcggca gacgggctcg
     1141 agctgctgcg tcacgacgcg gcgcacatca tggcggaggc cgtcaaagag ctgtacccgg
     1201 agactcaggt caccatcggc cccgccatcg aaaacggctt ctactacgac ttcgcgcgca
     1261 gcacgccgtt cacgcccgag gatctcgcga gcatcgagaa gcgcatgcac gaaatcgtcg
     1321 ctcgcgacga gaagatcgtg cgcgaggttt gggaccgcga cgccgccgtg aagtttttcc
     1381 tggagcaggg cgaagcctac aaggccgaga tcatccgcag catcccggcc ggcgagtcca
     1441 tcacgctgta tcgccagggc aacttcatcg acctgtgccg cggcccgcac ctgccgtcga
     1501 cggggcggct cggcgccggc ttcaagctca tgaagctcgc gggcgcgtac tggcgcggca
     1561 actccgacaa cgagatgctg cagcgcattt acggcacggt ttgggccgac gccaaagagc
     1621 tcaaggcgta cctcgatcgg ctcgaggaag ccgagcgccg cgaccatcgc aagcttggca
     1681 agcagatgga cctgttccac ttccaggacg aggccgtcgg cgccgtgttc tggcacacga
     1741 agggctggca gctgtttcgc acgctcgtcg agttcatgcg gtcgaagcag ggcgctgcgg
     1801 gttatcgcga ggtcaacacg ccggagctga tgtcacgctc gctctgggag gcgtcggggc
     1861 actggcacac cttcggcgag aacatgttca ctagcaaaac ggccgaggag caaaccttcg
     1921 tcgtcaagcc gatgaactgc cccggtcata tccaggtgtt caagagcgat ctgcgcagct
     1981 atcgcgacct gccttaccgc ctcgcggagt tcggtaaggt gcaccgctac gaaccttcgg
     2041 gcgcgctgca cggactcatg cgcgtgaggg cgttcacgca agacgatgcg cacgtgttct
     2101 gcacagaggc gcaaatcact gaagaagccg gcaaggtctg ccagctgttg ctcgacatct
     2161 accgaagctt cggcttcgac aacgtcatca tgaagtttgc ggaccggccg ccgaaacgcg
     2221 tcggcgccga cgaggtctgg gatcgagccg aggccgcgct gaaaagtgcc atggattcgc
     2281 tcggcttacc gtattccttg aacaaaggcg aaggcgcttt ctacgggccg aagatcgagt
     2341 tcgtgctgcg cgacgcgatc ggccgcgact ggcaatgcgg aacgctgcag gtcgacctca
     2401 acatgccggg acgtctcggc gcgacgtaca taggcgagga cggcaaccgc cacacgcccg
     2461 tcatgctgaa tcgcgcgatc ttcggctcgc tcgaacgttt catcggcatt ttgctcgagc
     2521 attacgcagg gtcgctgccg ctgtggctcg cgcccgtgca agtcgtcgtc acgacgatca
     2581 cgtcggatgc cgacgcgtac gcgcacgaag tcgtcgcgga gcttgcgcgt gcggggctgc
     2641 gtgccgaggc cgatctgcgc aacgagaaaa tcaactacaa ggtgcgcgag cacagtctc
//
LOCUS       DATLEE010000155         3086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9106119, whole genome shotgun sequence.
ACCESSION   DATLEE010000155 DATLEE010000000
VERSION     DATLEE010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9106119"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VM692_03340"
     CDS             complement(<1..741)
                     /locus_tag="VM692_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUQ51229.1"
                     /translation="MSKGMDAFQLPSGVAASRLGQGAWQIGEDKRKRAEELLALNVGI
                     DLGLNLIDTAEMYGHGRSEELVAEVIAGRRDRIYLVSKVLPENASRRGTITACERSLQ
                     RLKTDYLDMYLLHWRGSVPLEETLEAFTRLRERGSIRHYGVSNFDVSDLDEAAALPGG
                     RDVATNQVLYNLEQRGVEFELVPKSRERRIPLMAYSPLGSDSRRLRTHPVLKKLAARL
                     GATPSRLALAWLLQQPDVVVIPKASSEAH"
     gene            complement(738..1127)
                     /locus_tag="VM692_03345"
     CDS             complement(738..1127)
                     /locus_tag="VM692_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005349150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HUQ51230.1"
                     /translation="MATQRNVLLSAAGFSLLVATMAGAVGSHALTFADAQAQRSFETA
                     VQFQFFHGLGVVAIVLLGLAGRGGFVRGAAAWLMLAGTLLFCGSIYARALGAAPSIVA
                     LAPYGGVMFMVGWLAFAVSPWLGEREL"
     gene            complement(1577..3007)
                     /locus_tag="VM692_03350"
     CDS             complement(1577..3007)
                     /locus_tag="VM692_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HUQ51231.1"
                     /translation="MRATRTLVLLTSFLAVAPHARGAPALLRAVHSLDEPRGYCLDIP
                     GAGPTLNLDGPLQAHTCKEQPSLDDQLFEMRAEQIQSHDRCLTAEALEPGSALVLRPC
                     SDSRQQRWTFADGRVTAAARRQLCVTLAAVRGEPWGTPRLISPVYRRQALALADCDAS
                     HDTRQIFNWVPREEAAAEPSTADVQRTGMPADVAAALAAFGHEFDGNIAQQTAALYAG
                     QPHVYAAAELSVAKDLAYGAHERQRLDVHTRANRPAEPVPVIVVFHGGGLIGGSRAAT
                     VNVAEYFASLGYVGVNGGYRLAPEAKWPEGARDVGAAVTWLRDHVAEYGGDPAKIFVA
                     GISTGALHAATYVFRPELVPGAARPAGAILVSGPYTFDFAAAGRGELAYFGEDRSRWP
                     AMVVPGNVTSAAVPVLMTTAEWDNERYTTSYALLFHELVTKHGIVPRYFQSLGHNHSS
                     QLLSVGTSDTSVSSVIVDFIERTTGR"
BASE COUNT          493 a         1035 c         1073 g          485 t
ORIGIN      
        1 gtgggcctcg ctcgacgctt tcggaatcac gacgacgtcg ggctgctgca gcagccacgc
       61 gagcgcgagg cgcgatggtg tcgcgccgag gcgtgccgcg agcttcttca gcacgggatg
      121 cgtgcgcaga cggcgcgagt cgctgccgag cggcgagtac gccatcagtg ggatgcggcg
      181 ttcgcggctc ttcggtacga gctcgaactc gacgccgcgc tgctccaagt tatagagcac
      241 ctgattcgtc gcgacgtcgc ggccacccgg cagcgccgcg gcctcgtcga ggtcggacac
      301 gtcgaaattg ctgacgccgt aatggcggat cgagccgcgt tcgcgtaacc gcgtgaacgc
      361 ctcgagcgtc tcctcgagcg gaacggagcc gcgccagtgc agtaaataca tgtcgagata
      421 gtcggtcttg agtcgctgca agctgcgctc gcacgccgtg atcgtgccgc ggcgcgacgc
      481 gttctcgggc agcaccttgc tgacgagata gatccggtcg cgccggcccg cgatgacctc
      541 ggcgaccagc tcctcggagc ggccgtgccc gtacatctcg gccgtgtcga tgagattgag
      601 cccgaggtcg atgccgacgt tcagcgccaa cagctcctcg gcacgcttgc gcttgtcctc
      661 gccgatctgc cacgcgcctt ggcccaaccg cgaggcggcc acgcccgacg gcaactgaaa
      721 ggcgtccatg cctttgctca tagctctcgt tctcctaacc aggggctgac tgcgaatgcg
      781 agccagccga ccatgaacat gacgccgcca tacggggcga gcgcgacgat gcttggggcc
      841 gcgccgagcg cgcgtgcgta aatgctgccg cagaacagca gcgtgcccgc gagcattagc
      901 caagcggctg cgccgcgcac gaagccgccg cggcccgcga gccctagcag cacgatcgcg
      961 acgacgccaa ggccgtgaaa gaactgaaac tggaccgcag tctcgaagct gcgctgcgct
     1021 tgcgcgtcgg cgaacgtcag cgcgtggcta cccaccgccc cggccatcgt cgccacgagc
     1081 agcgagaagc ccgcggcgga caggagcacg tttcgttgcg tcgccacacg cttagcttac
     1141 ggcatccgct ctcgcggtga cgccagcgcc cggagtcgcg ctcaccgtgc accgacgcgc
     1201 gccgggcgca tgcgccgttc gacggtgacg gcggaattgg tgggcccggt aggacttgaa
     1261 cctacgacca agggattcac tttacccgcc gtttccggcg gaagtggact atctcttcac
     1321 ccgccgagtc gctcgggtag ggtgcgggac gctcatgcct gtcattaaga gcgctcgagc
     1381 tctcaggtag tctctgcacc gtccggcggt gtaccgccgg cttggctcag ggttgccggc
     1441 gcgaaatgcg ttgcgggttc cctgaattca tcccgtccac ttcgcggctt gtcgccgcga
     1501 aggcaccttt ctcgatgagt cccctgctct aaccaactga gctacaggcc cacgcgcgta
     1561 tggtacaccg gccggttcac cggccggtcg tccgttcgat gaagtcgacg atgacgctcg
     1621 agacgctggt gtcgctcgtg ccgacggaca gcaattgcga cgagtggttg tgtcccaagc
     1681 tctggaagta gcgcggcacg atgccgtgct tggtcacgag ctcgtggaac agcagcgcat
     1741 acgaagtcgt gtagcgctcg ttgtcccact cggccgtcgt catgagcacg gggacggcgg
     1801 cgctcgtgac gttgcctggg accaccatcg cgggccaacg gctgcggtcc tcgccgaagt
     1861 acgcgagctc gccgcggccg gcggccgcga aatcgaacgt ataggggccc gacacgagga
     1921 tggcgccggc cgggcgtgcg gcgcccggca cgagctcggg acggaagacg tacgtcgcgg
     1981 cgtgcaaggc gccggtcgaa attccggcca cgaagatctt ggccggatcg ccgccgtact
     2041 cggcgacatg gtcgcgcaac cacgtgaccg cggcgccgac gtcgcgcgcg ccttcgggcc
     2101 atttcgcctc gggtgccaag cggtaaccgc cgttcacgcc gacgtagccc aagctcgcga
     2161 agtattccgc gacgttgacg gtcgcagcgc ggctgccgcc gatgagcccg ccgccgtgaa
     2221 acactacgat cacgggcacc ggctcagcgg ggcgattggc gcgcgtatgg acgtccaagc
     2281 gctggcgctc gtgcgcgccg taggcgagat ctttcgcgac actgagctcc gcggccgcgt
     2341 aaacgtgcgg ctgcccggca tagagcgcag ccgtttgctg cgcgatgttg ccgtcgaact
     2401 cgtgaccgaa cgccgccagt gcggccgcga cgtcggccgg catgccggtg cgctggacgt
     2461 cggccgtgct cggctcggcc gccgcctctt cgcgcggcac ccagttgaat atttggcgcg
     2521 tgtcgtggct cgcgtcgcag tcggcgagcg ccagcgcctg gcggcgatac acgggcgaga
     2581 tgaggcgcgg cgttccccac ggctcgccgc gcacggccgc cagcgtcacg cacagctgac
     2641 gccgtgccgc ggcagtcacg cggccgtcgg caaaggtcca acgttgctgg cgggagtccg
     2701 agcatgggcg cagcacgagc gcgctgcccg gctcgagcgc ttcggcggtc aggcaccggt
     2761 cgtgcgactg gatctgctcg gcgcgcatct cgaacagctg gtcgtcgagg ctgggctgct
     2821 ccttacaggt gtgggcttgg agcgggccgt cgagattcag cgtggggccg gcaccgggta
     2881 tgtcgaggca gtagccgcgc ggctcgtcga gcgagtgaac ggcccgcaag agcgccggcg
     2941 cgccgcgagc gtgcggcgct acggcgagaa atgaagtcag aaggacgagg gtacgcgtag
     3001 cgcgcataca ccgtctcccg ccgagaggtg cgggcagtat gcgacgaagc tgaacggagg
     3061 cgccagccgc gatggttgcg tccgac
//
LOCUS       DATLEE010000156         3440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1660932, whole genome shotgun sequence.
ACCESSION   DATLEE010000156 DATLEE010000000
VERSION     DATLEE010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660932"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VM692_03355"
     CDS             <1..900
                     /locus_tag="VM692_03355"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51232.1"
                     /translation="TTENPSFELNSALLSRLRVFVLEPLAPDAIRAIVVRAQRQLAAE
                     KPATRELSSDAVELLVRHADGDARRALNATEAVLGHVARGKRSGTPATADEVAAVLEK
                     RIAQFDKAGEQHYNVMSALHKSVRGSDPDAALYWFARMVEGGEDLLYVARRVVRMAVE
                     DIGLADPRGLSIALAAKDAYHFLGSPEGDLAIAEAIVYLATAPKSNNIYGAYGAALAA
                     AKEHPAEPVPLHIRNAPTKLMKDIGYGGGYRYDHAEGGHAAGQEYLPDALRGARWYEP
                     GSTGYEKTIAERLAWWRQHKNET"
     gene            935..2014
                     /locus_tag="VM692_03360"
     CDS             935..2014
                     /locus_tag="VM692_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUQ51233.1"
                     /translation="MNQTNIERAFFFALLLAVTLAFFWLIKSFIQPIFWAVALGILIY
                     PVHADLQRRLGKRESLAAIISVITVVIVVILPLIGLGAAVAREGSALYERLESDGGVA
                     SIYSRIQESQPRITALIERVGIDPARLEEQVQSAAVAASRYVAQRAVDIGSGTLRGTI
                     FFFLMLYLLFFFLRDGPRMLDALVRALPLGDQRERHLLRRFAEVSRATIKGTLVVGIV
                     QGAIGGIAFAVLGVGAPVLWGVVMALLSILPAVGTALVWVPAAIFLLFNDHVFGGIAL
                     IVVGVLVIGSVDNVLRPILVGRDTRMADYLILLSTLGGLAGFGLAGIIIGPIIAAFFL
                     SVWHMAEKEFHAAEPAPEPEPPTEG"
     gene            2113..2526
                     /locus_tag="VM692_03365"
     CDS             2113..2526
                     /locus_tag="VM692_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ51234.1"
                     /translation="MAAIDTRNELLVREFFDTLSSGDLEALRPLLHADASWEATGKSI
                     PGAGITRGRDKIIDEFLKPVRGLFEPGDPKVIALRVFAKGEWVAAETEGVGMLASGKE
                     YHNRYAWIIEIKDDKVFALREYMDTHYILTQLDML"
     gene            2523..>3440
                     /locus_tag="VM692_03370"
     CDS             2523..>3440
                     /locus_tag="VM692_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUQ51235.1"
                     /translation="MSTTYDIVTVGSGHNGLVAAAYLAAAGKKVLVLERNPWFGGGVV
                     TRALTVPGYLHDQHSMAHIFIQANPLLKNDELKLKAKHGLQYVFPELPMMSVFEDGTT
                     LGLYRDREKSCAEIAKFSQKDAETYRWLAGKAAGYLPMITASLYAPPAPLGATYALMD
                     QSREGRELWRYMQMSSHDLLCSLFEHDKVRMHFARVAGENLVSPDEKATGIGVFVFVA
                     FLEAYGIGVPVGGSGKLTDALIASIKSHGGDVVANADVARITTKNGRASGVQLQDGRS
                     FDAKDGVVAALHPHDLGAMVDGVAPEVVKA"
BASE COUNT          579 a         1169 c         1108 g          584 t
ORIGIN      
        1 acgaccgaga atccgtcgtt cgagctgaac tcggcgctgt tgtcgcgcct gcgagtgttc
       61 gttctcgagc cgctggcgcc ggacgccatc cgcgcgatcg tcgtgcgtgc acagcgtcaa
      121 ctcgcggcag agaagcccgc gacgcgcgag ctttcgagcg acgccgtcga gctgctcgtg
      181 cggcatgccg acggcgacgc gcgacgcgcg ctgaacgcga cggaagccgt gctcggccat
      241 gtcgcgcgcg gcaaacgcag cggcacgccg gcgacggccg acgaggtcgc ggccgtgctc
      301 gaaaagcgca tcgcgcaatt cgacaaggcc ggcgagcagc actacaacgt catgtcggcg
      361 ctgcacaaat ccgtgcgcgg cagcgatccc gacgcggcgc tgtactggtt cgcgcgcatg
      421 gtcgaaggcg gcgaggacct gctgtacgtt gcgcggcgcg tcgtgcgcat ggccgtcgag
      481 gacatcgggc tcgccgatcc gcgcggcctg tcgatcgcct tggcggccaa ggatgcttat
      541 cacttcttgg gctcgcccga gggcgacctc gcgatcgccg aagccatcgt ttatctcgcg
      601 acggcgccga agtcgaacaa tatttacggc gcgtacggcg ccgcgctggc cgcggcgaaa
      661 gagcaccccg ccgagcccgt gccgctgcac atccgcaatg cgccgacgaa gctcatgaag
      721 gacatcggct acggcggggg ttaccgctac gatcacgccg aaggcggcca cgcggcgggc
      781 caggagtacc tgcccgacgc gctgcgcgga gcgcgctggt acgagcccgg cagcacgggt
      841 tacgagaaaa cgatcgccga gcgcctcgcc tggtggcgcc agcacaagaa cgaaacttga
      901 acagccaccc gccctgcctg tctcgggagc cgcgatgaat cagaccaaca tcgaacgtgc
      961 gttcttcttc gcgctgctgc tggccgtcac gctcgcgttc ttctggctga tcaagagctt
     1021 catccaaccg atcttctggg ccgtcgcgct cggcatcctc atctaccccg tgcacgccga
     1081 tctgcagcgg cgcttgggca agcgcgagtc gctcgctgcg atcatcagcg tcatcacggt
     1141 cgtcatcgtc gtgatcctgc cgttgatcgg cctcggcgcc gccgtcgcgc gcgaaggctc
     1201 tgcattgtac gaacgcttgg aaagcgacgg cggcgtggcg agcatctact cgcgcattca
     1261 ggagagccag ccacgcatca cggcgctcat cgaacgcgtc ggcatcgatc cggcccgcct
     1321 cgaagagcaa gttcaaagcg ccgccgtcgc cgcgagccgc tacgtcgcac aacgcgccgt
     1381 cgacatcggt tccggcacgt tgcgcggcac gattttcttc ttcctgatgc tgtatctctt
     1441 gttcttcttc ttgcgcgacg ggccacggat gctcgatgcg ctcgtgcgcg ctctaccgct
     1501 cggcgatcag cgcgagcgcc atctgctgcg acgattcgcg gaggtctcgc gcgcgacgat
     1561 caaaggcacg ctcgtcgtcg gcatcgtcca gggcgccatc ggcggcatcg cgttcgcagt
     1621 cctcggcgta ggcgcgcccg tgctctgggg cgtcgtcatg gcgctgctca gcattctacc
     1681 ggccgtgggc acggcgctcg tgtgggtgcc ggccgccatc tttctattgt tcaacgacca
     1741 cgtgtttggc ggcatcgcac tgatcgtcgt cggcgtgctc gtgatcggct ccgtcgacaa
     1801 cgtgctgcgc ccgattctcg tcgggcgcga cacgcgcatg gcggactatc tgatcctgct
     1861 gtcgacgctc ggcggcctcg caggcttcgg actcgcgggc atcatcatcg gcccgatcat
     1921 cgccgcgttc ttcttgagcg tctggcacat ggccgagaag gaatttcacg ccgccgagcc
     1981 ggcgcccgag cccgagccgc cgacggaagg ctgacggctc gagcgagctc gagctcctcc
     2041 ttcttccggc aacagcgagg cagcgggtat gctacaagtc gcaacgtcgg gcgcttccgc
     2101 aacttcccgc caatggccgc aatcgacacc cgcaacgagc tcttagtgcg cgagttcttc
     2161 gacacactgt cgagcggcga tctcgaggcg ctgcgtccgt tgctgcacgc ggacgcgagc
     2221 tgggaagcga ctggcaaatc gattccgggc gccggcatca cgcgcggccg cgacaagatc
     2281 atcgacgaat tcttgaagcc ggtccgcggc ctgttcgagc ccggcgaccc gaaggtcatc
     2341 gcgctgcgcg tcttcgccaa aggcgaatgg gtcgcggccg agaccgaagg cgtcggcatg
     2401 ctcgcgagcg gcaaggaata ccacaatcgc tatgcctgga taatcgagat caaggacgac
     2461 aaggtgttcg ccttgcgcga atacatggac acccactaca tcctcacgca actcgacatg
     2521 ctatgagcac cacctacgac atcgtcacgg tcggttccgg tcacaacggc ctcgtcgccg
     2581 ccgcatacct cgcggccgcg ggcaagaagg tgctcgtgct cgagcgcaac ccctggttcg
     2641 gcggcggcgt cgtcacgcgc gcgctgaccg tgcccggcta tctccacgat cagcactcga
     2701 tggcccacat cttcatccag gcaaacccgc tgctgaagaa cgacgagctc aagctcaaag
     2761 ccaagcacgg tcttcagtac gtgttccccg agctgccgat gatgtccgtg ttcgaggacg
     2821 gcacgacgct cggcctctat cgcgatcgcg agaagagctg cgcggagatc gccaagttct
     2881 cgcagaagga tgccgagacc tatcgctggc tcgcgggcaa agcggccggt tatctgccga
     2941 tgatcacggc gtcgctgtac gcgcctccgg cgccgctcgg cgcgacgtat gcgctgatgg
     3001 atcagagccg cgagggccgc gagctctggc gttacatgca gatgagctcg cacgacctgc
     3061 tctgcagctt gttcgagcac gacaaggtcc gcatgcactt cgcgcgcgtc gccggcgaga
     3121 acttggtctc gcccgacgag aaggccaccg gcatcggcgt gttcgtcttc gtcgcgttcc
     3181 tcgaggccta tggcatcggc gtgcccgtcg gcggctccgg caagctgacg gacgcgctga
     3241 tcgccagcat caagtcgcac ggcggcgacg tcgtcgcgaa tgccgacgtt gcgcgaatca
     3301 cgacgaagaa cggccgcgcc tcaggcgtgc aattgcagga cgggcgctcg ttcgacgcga
     3361 aggacggcgt cgtcgcggcg ctgcacccgc acgatttggg agccatggtc gacggcgtcg
     3421 cgcccgaggt cgtcaaagcg
//
LOCUS       DATLEE010000157         5238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6047795, whole genome shotgun sequence.
ACCESSION   DATLEE010000157 DATLEE010000000
VERSION     DATLEE010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6047795"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VM692_03375"
     CDS             <1..738
                     /locus_tag="VM692_03375"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HUQ51236.1"
                     /translation="GSHQRWSDDSEHGRRGTGYVYSSQFISEEAAERELRGYVGQQAE
                     GLNARHLKMRIGHNRKFWVRNCVAIGLAGGFIEPLESTGIGLISQGLALLAFHFPTPD
                     FPEPLAESYNGAMTRLYEHIREFIVLHYCTTRREDTEFWRANRNNPHMPRRLQSLLEQ
                     WRHRAPDAYDNLSGFDFFHPTSFQYILAGMGHLAAPSDYVRNRLPRDFRAHMRRRIGE
                     VHASALAASVGHIEFLRAQYGGERPRG"
     gene            complement(763..2253)
                     /locus_tag="VM692_03380"
     CDS             complement(763..2253)
                     /locus_tag="VM692_03380"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HUQ51237.1"
                     /translation="MKNLAVLVAMLAVSFAAHAAPDCAALKTFSLPTYDIVITNAAVV
                     PAAPPVPAHCRVDGTIHDRIGAEGKPYGIGFGLALPTNWNQRFLFQGGGGYNGLVRPP
                     LGVAAVGAQTGLARGFAVVSTDTGHKSEQNFDRTYDADQQASIDFNHFAVGDVTVVAK
                     QIVAKYYGEAPEYSYFAGCSTGGREAMVMTQRYPFYFDGVVSGDPAIHVGYSGLGNAW
                     LRAAFNEAAPKDANGRPQPQLLFSDADKKVLVDGVLAACDADDGVRDGMVFNSRACDF
                     DPADLVCSGSKTTECLSPAQVGAIEKGFAGPRNSRGKLIYRMSPNDAGVAAFLPSATP
                     PSANAALPTSVDVDGRLFEMLENPIAQMTDSIWTNLSSFSGHGGKLLFYHGMADPTFH
                     GTDTIDYYERMAAANGGMDKVQGWSRFFAVPGMLHCRGGDSALDEFDLLSAVMNWVEK
                     GVAPESVVATGKAFPGRSRPLCAYPKHAHYTGNGDPEDARNFVCRE"
     gene            2471..4105
                     /locus_tag="VM692_03385"
     CDS             2471..4105
                     /locus_tag="VM692_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HUQ51238.1"
                     /translation="MRFQSSRRDFVQGSLAAALSTGFMRSSWAQTSANTPLTVEAETA
                     YGRVHGTEVRGIKSFKGIPYAASTAGKNRFMPPLKPAAWTGVRDALDYGARAPQQQPR
                     PRGEADEQGATPGQQESEDCLLLNVWTPALADGGKRPVIMWCHGGGFNTGSGAAPVND
                     GTNLGLRGDIVTVTTNHRLNVLGYSYLGEIGGKDFEQSAALGVLDLVAALEWIRDNIE
                     RFGGDPGNVTIIGQSGGAQKVSTLLTMPSAKGLFHRAVVMSGSALQLLERDQAARVTH
                     ELVAELGLAKPTIAQLQEVPIDKLMSAYFATVAKLNSQRLGQGFRPAVDGTAITQHPF
                     HPAASRVSPDVPVIFSHTSGEATFRADERLFTLDEAGMRKELTNRTGDKTDALVALYR
                     KLYPKAMPPDLYFLIASDHQYGGGAMKAAERRSVPGFAPAYLYYWTWESPLEGGKYRA
                     QHTVDIAFAFDNVKASRLTAGAPDAQALADQVSEAFIAFFRTGDPNTPKSKLPAWPRY
                     DATNRPTMVFSSTPALRNDPIREQRLAMWDALGLAG"
     gene            4173..4754
                     /locus_tag="VM692_03390"
     CDS             4173..4754
                     /locus_tag="VM692_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HUQ51239.1"
                     /translation="MNKPDATLKDFIHVERGIIPRDLCAQLVAAIEPREWRQHGWYGA
                     AQGKVVTHATMELEVQECSTEHFELLTPWVVKAGRAYSAKFSFPSELTQPIARRFSAI
                     RFNRYSPGQIMRQHHDHIHSLFDGVYKGIPVFSMVGNLNDGYEGADLYFWDGYKVPLG
                     VGDICLFPSLFMYPHGVTEATRGRRYSFVCWYW"
     gene            4901..>5238
                     /locus_tag="VM692_03395"
     CDS             4901..>5238
                     /locus_tag="VM692_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUQ51240.1"
                     /translation="MGARKGQIRDLLRAARARLDPAEFGLRGSGRRKVPGLRREDVAV
                     LAQVSLKWYTWLEQGRELNFSDELLCRVARVLRLSHSEQAYLVALTRRSVSPDVTLAA
                     SSTSEWLRRTV"
BASE COUNT          917 a         1697 c         1723 g          901 t
ORIGIN      
        1 ggcagccacc agcgttggag cgacgacagt gagcacgggc gccgcggcac gggctatgtg
       61 tattcgagtc aattcatctc ggaggaggcg gccgagcgcg agctgcgcgg ctacgttggc
      121 cagcaggccg aaggcctcaa cgcacggcat ctgaaaatga ggatcggcca caaccgcaag
      181 ttctgggtca ggaactgcgt cgcgatcggg ctcgccggcg gcttcatcga gccgctcgag
      241 tcgacgggga ttgggctgat ctcacaaggg ctcgcgctgc tggcgttcca cttcccgacg
      301 cccgattttc cggagccgct ggccgagagc tacaacggcg caatgacgcg cctctatgag
      361 cacattcgcg agttcatcgt gctgcactac tgcacgacgc gccgcgagga cacggagttc
      421 tggcgcgcca acagaaacaa cccgcacatg ccgcggcgct tgcagtcatt gctcgagcag
      481 tggcgccatc gcgcgccgga cgcgtatgac aacttgagcg gcttcgattt ctttcatccg
      541 acgtcgttcc agtacatttt ggccggcatg gggcacctcg ctgcgccgag cgactacgtg
      601 cgcaaccgcc taccgcgcga cttcagagcg cacatgcggc gacggatcgg cgaagtgcac
      661 gcaagcgctc tggccgcgag tgtcgggcac atcgagttcc tgcgagcgca gtacggcggt
      721 gagaggccac gcggctaagc cggcgggccg aaagtttacg aatcactcgc gacaaacgaa
      781 gttgcgtgca tcctccggat cgccgttgcc cgtgtagtgc gcatgcttcg gatacgcgca
      841 gagcggccgg ctgcgcccgg ggaatgcctt gccggtcgcg acgaccgatt cgggcgccac
      901 tcctttctcg acccagttca tgacggcgga cagtagatcg aactcgtcga gcgcagagtc
      961 gccgccgcgg cagtgcagca tgccgggcac cgcgaagaag cggctccagc cctgcacttt
     1021 gtccatgccg ccattcgctg cggccatgcg ctcgtagtag tcgatcgtgt ccgtgccatg
     1081 aaacgtggga tcggccatgc cgtgatagaa cagcagcttg ccgccgtggc ccgagaacga
     1141 gctcaggttc gtccatatgg agtcggtcat ttgagcgatc ggattctcga gcatctcgaa
     1201 cagccgtccg tcgacatcga cgctcgtcgg aagcgctgca ttggccgagg gcggtgtggc
     1261 gctcggtaga aacgctgcga caccggcgtc gttcgggctc atgcggtaga tcagcttgcc
     1321 gcgtgaattg cgcggtcccg cgaaaccctt ctcgatggcg ccgacttgcg ccggtgacag
     1381 gcactccgtg gtcttcgagc cgctgcagac gaggtcggcg ggatcgaaat cgcacgcgcg
     1441 cgagttgaag accatgccgt cgcgcacgcc gtcgtcggca tcgcaggctg ccagcacgcc
     1501 gtcgacgagc acttttttgt cggcgtcgga gaacaacagc tgcggttgcg gccgaccgtt
     1561 tgcatctttc ggggccgctt cgttgaacgc cgcgcgcagc cacgcgttgc cgagccccga
     1621 gtagccaacg tgaatcgcgg gatcgcccga caccacgccg tcgaagtaga acgggtaacg
     1681 ctgcgtcatg accatcgctt cgcgcccgcc ggtcgagcag cccgcgaagt acgagtactc
     1741 cggcgcttcg ccgtagtact tcgctacgat ctgcttcgcc accacggtca cgtcgccgac
     1801 tgcaaagtgg ttgaagtcga tgctcgcttg ttgatcggcg tcgtaagtgc ggtcgaagtt
     1861 ttgttcgctc ttgtgccccg tgtcggtcga cacgaccgcg aagccgcgtg cgagaccggt
     1921 ctgcgcgccg acggccgcga caccgagcgg cgggcggacc aagccgttgt agccgccgcc
     1981 gccctggaac aggaagcgct gattccaatt cgtcggcaat gccaggccga agccgatgcc
     2041 gtacggtttg ccctcggcgc cgatgcggtc gtggatcgtg ccgtcgacgc ggcaatgcgc
     2101 cggcaccggc ggtgcagcgg gaacgaccgc ggcgttcgtg atgacgatgt cgtacgtcgg
     2161 cagcgagaac gtcttcaacg cggcgcagtc gggcgcggcg tgtgccgcga aactcactgc
     2221 gagcatggcg accaatacgg ctaggttttt catggggcac tcggcactta gatgtgtcgc
     2281 tgagtatcgg cggctgcttg agccgctgcc agggtgctcg cgagggatag cattggctct
     2341 acccgtcgta gagcgcgcgc cccttggaag tccccgaagg gggacgtctt gcgggactct
     2401 tcgtcgtcgc agaacatgtg tcggcacgtc gcgatccgct cgtgcgtccg ctagcaaggg
     2461 gggagaagct atgcgcttcc aaagttcacg ccgcgacttc gtccaagggt ctctcgccgc
     2521 agcgctgtcc acgggcttca tgcgctcgag ctgggcacag acgagcgcga acacgcccct
     2581 tacggttgag gccgaaacgg cctacggccg cgtccacggc accgaggtgc gcggcatcaa
     2641 gagcttcaaa ggcattccgt acgccgcgag cacggccggc aagaaccgct tcatgccgcc
     2701 gttgaagccg gccgcgtgga ccggcgtgcg cgacgctctc gactacggcg cgcgagcgcc
     2761 gcaacaacaa ccgcggcccc gcggtgaggc cgacgagcaa ggcgctacgc cgggacagca
     2821 agaaagcgaa gattgcttgt tgctgaacgt ttggacgcca gcgctcgccg acggcggcaa
     2881 gcggcccgtg atcatgtggt gccatggcgg cggcttcaac acgggctccg gtgcagcgcc
     2941 cgtcaacgac ggcacgaatc tgggcctgcg cggcgacatc gtcacggtca cgacgaacca
     3001 ccggttgaac gtcctcggtt attcgtatct cggcgagatc ggcggcaagg atttcgagca
     3061 gtcggccgcg ctcggcgtgc tcgatctcgt cgcggcgctc gaatggatcc gcgacaacat
     3121 cgagcgcttc ggcggcgacc ccggcaacgt cacgatcatc ggtcagtccg gcggcgcgca
     3181 aaaagtctcg acgctgttga cgatgccctc ggcgaagggc ttgttccatc gcgccgtcgt
     3241 catgagcggc tcggcgctgc agcttctcga gcgcgaccag gccgctcgcg tgacgcacga
     3301 gctcgtcgcg gagctcggcc tcgcaaagcc gacgatcgcg cagctgcaag aagtgccgat
     3361 cgacaagctc atgagcgcct acttcgcgac cgtcgcgaag ctcaactcgc agcgtctcgg
     3421 tcaaggcttt aggccggccg tcgacggcac ggcgatcacg cagcacccgt tccatccggc
     3481 cgcgtcgcgt gtatcgccgg atgtgcccgt gatcttcagt cacacgagcg gtgaggcgac
     3541 gttccgcgcc gacgaacgcc tgttcacgct cgatgaggcc ggcatgcgca aagagctcac
     3601 caatcgcacc ggcgacaaga ccgacgcgct ggtagcgctg tatcgcaagc tctatccgaa
     3661 ggcgatgccg ccggatctgt acttcctgat cgcgagcgat catcagtacg gcggcggtgc
     3721 gatgaaggcc gcggagcgcc gctcggtgcc gggcttcgcg cccgcgtact tgtactactg
     3781 gacgtgggag tcgccgctcg aaggcgggaa gtaccgcgcc cagcacacgg tcgacatcgc
     3841 gttcgcgttc gacaacgtga aagcgtcgcg gctgacggca ggcgcgccgg atgcacaagc
     3901 gctcgcggat caagtcagcg aagcgttcat cgcgtttttc cgcacgggcg atccgaacac
     3961 gccgaagtcg aagttgcccg cttggccgcg ctacgacgcg acgaacaggc cgacgatggt
     4021 gttctcgagc actccggcgt tacgcaacga tccgatacgc gagcaacggc tggcgatgtg
     4081 ggacgcgctc ggcctcgccg gctagccgag caccccgcgc tccatcgtat ccttccggta
     4141 cgggctcacc cgatacggcg ggaggcgcag ccatgaacaa gcctgacgca acgctgaagg
     4201 atttcattca cgtcgagaga gggatcattc cccgcgacct ctgcgcccag ctcgtcgccg
     4261 cgatcgagcc gcgcgagtgg cgtcagcacg gctggtacgg cgccgcgcag ggcaaggtcg
     4321 tcactcatgc gacgatggag ctcgaagtcc aggaatgctc gacggagcac ttcgagctgc
     4381 tgaccccgtg ggtcgttaag gccggcaggg cctacagcgc gaagttctcg ttcccgtcgg
     4441 agctcacaca gccaatcgca cggcgattca gcgcgattcg cttcaaccgc tattcccccg
     4501 gccagatcat gcgccagcac cacgaccaca tacacagcct gttcgacggg gtgtacaagg
     4561 ggatcccggt gttctcgatg gtcggcaatc tgaatgacgg ctacgagggc gccgatctct
     4621 acttctggga cggctacaag gtgccgctcg gcgtcggcga tatctgcctg tttccgtccc
     4681 tgttcatgta tccgcacggc gtgacggaag cgacgcgcgg caggcgctac agcttcgtct
     4741 gctggtattg gtgaggccgc gggcggtcgc ctgaacgaga gccgttctcg ttcttaagaa
     4801 cgagaacact gggccgaacc gttcggtttt gcggcccggc tgcgcctggc atagcgtttg
     4861 ccttgccgcg caacgcgttc ttccgagggg ggaaagaatg atgggcgcac gcaaaggtca
     4921 aattcgcgat ctattacggg ccgcgcgtgc acggctcgat ccggcggagt tcggcctgcg
     4981 cggctccggc cggcgcaaag tgccgggcct ccgacgcgaa gacgtcgccg tgctcgcgca
     5041 agtgagcctc aagtggtaca cgtggctcga gcaaggtcgt gagctcaatt tttccgacga
     5101 gctgctctgc cgcgtggcac gtgtcctgcg cctctcgcat tccgagcagg cgtatctcgt
     5161 cgcgctgacg cgtcgcagcg tttcgcccga cgtgacgctc gccgcgtcga gcaccagcga
     5221 atggctgcgc cgcacggt
//
LOCUS       DATLEE010000158         7610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3896913, whole genome shotgun sequence.
ACCESSION   DATLEE010000158 DATLEE010000000
VERSION     DATLEE010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3896913"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..562)
                     /locus_tag="VM692_03400"
                     /pseudo
     CDS             complement(170..562)
                     /locus_tag="VM692_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018948928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
     gene            complement(583..1212)
                     /locus_tag="VM692_03405"
     CDS             complement(583..1212)
                     /locus_tag="VM692_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HUQ51241.1"
                     /translation="MTVIANRRSVMTLFSKPTDPWSHRTRLVLAEKSITIDIVDVDDG
                     NLPEDLLDLNPYNSVPTLVDRDLVLYDSRVIMEYLDERFPHPPLMPVDPVTRAQFRLA
                     LFRIEHDWYSLAGEIVGGLERKPTTRAVKALEESILSSVEIFAAKKFFLSDEFSLVDA
                     TIAPILWRLPVFGIKLPPQARPIQRYMDDVFSRPSFQASLTDMERELRQ"
     gene            complement(1256..1984)
                     /locus_tag="VM692_03410"
     CDS             complement(1256..1984)
                     /locus_tag="VM692_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HUQ51242.1"
                     /translation="MRSFVIAVVFGVALAGPVAAQHEAELEPANNNVANTASLQRGAK
                     YFVNYCLGCHSAKYVRYNRLGQDLQLSDQQLIDNLMFTGERPFDTMANAMRPEDSARW
                     FGKSPPDLSLIARSRGPDYIYTFLRSFYADPSRPTGVDNVVLPGTAMPHVLWQLQGIQ
                     RGVFKADEHGLEVFERFDVARPGELGVEEFDNVVRDIVNFLDYIGEPIKRERQQLGIR
                     VIGFLLVFLLIAYMLKKEIWKDVH"
     gene            complement(1981..3153)
                     /locus_tag="VM692_03415"
     CDS             complement(1981..3153)
                     /locus_tag="VM692_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HUQ51243.1"
                     /translation="MTKLIREHATEYYASKNFNFWYGFGVIATFVLVLQLVTGIFLTM
                     NYKPAAADAFASVEYIMRDVEWGWLIRYLHSTGASAFFIVVYLHMFRGLLYGSYKKPR
                     ELVWIIGMFIYLSLMAEAFFGYLLPWGNMSYWGAQVIVSLFGAIPVIGNGLSEWIRGD
                     FYISDITLNRFFAFHVIAVPLMLVLLVVVHIMALHEVGSNNPDGIEIKEHKGADGHPI
                     DGVAFHPYHTSKDLVIIIIFLILFSAVVFFAPDMGGYFLEHANFEPANTLATPEHIAP
                     VWYFTPFYAILRAVPNKLLGVALMGSAVLLLFFLPWLDRCRVKSIRYRSLGYKLALMG
                     FAITFVTLGYLGLQPAVGNTPTLSLIFTILYFSFFILLPFYTRNEKTKPVPTRVTW"
     gene            complement(3238..3828)
                     /gene="petA"
                     /locus_tag="VM692_03420"
     CDS             complement(3238..3828)
                     /gene="petA"
                     /locus_tag="VM692_03420"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HUQ51244.1"
                     /translation="MAESTGFTRRHFLTVATVVTGGAGAIATAVPFVSSFKPSARAQA
                     LGAPVTVDISKLESGALLRVEWRGRAIYIVHRTDEMLTSLAKDAGHLKDPESEESVQP
                     SYATNEHRSVRPAFLVLEGVCTHLGCAPMPRFEVAPADLGPEWPGGFYCPCHGSKFDL
                     AGRVFDGVPAPTNLPVPPYRYINDNTILIGSDTGNS"
     gene            4022..5095
                     /locus_tag="VM692_03425"
     CDS             4022..5095
                     /locus_tag="VM692_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUQ51245.1"
                     /translation="MPPVLKVAAFIAQSVAIGLALAFIAVVVKPDLINRAPTTSTQWS
                     YAQAVAASAAAVVNINTSRVFNLPNNRRNLRVRREGLGSGVVIDREGYVVTNYHVIQG
                     ADEIGVQLADGRVAVPTLVGTDPDTELALLKIDLPDLPEIRLGRSDTLKVGDVVLAIG
                     NSLGLSQSVTMGIVSATGRGQLVGIPFEDFIQTDAAINAGNSGGALVNARGELVGINT
                     AVLSAADGQDTPEGLSFAIPVNLVRGVMEQLITHGRVIRGYLGVDEAVDFPASQAKQL
                     GIEGSAVQITRASGPAAAAGLRADDVLTHLNGERMYSGQQALNFVASLQPGDRLEIRG
                     TRADGTPFTTEAVLEERPTPGLN"
     gene            complement(5099..6358)
                     /gene="murA"
                     /locus_tag="VM692_03430"
     CDS             complement(5099..6358)
                     /gene="murA"
                     /locus_tag="VM692_03430"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HUQ51246.1"
                     /translation="MDKLAIVGGRALDGDVRVSGAKNAALPILAATLLADGAVTLGNV
                     PRLNDIATTLKLLKRMGVAAEHRDDSSVVVDSGEIKEFLAPYELVKTMRASILVLGPL
                     LGRFGHADVSLPGGCAIGARPVNLHVMGLRAMGASISIDNGYIRARATKLKGARLVLD
                     TVTVTGTENLMMAAVCAAGETVIENAAREPEIGELAAFLMAMGAKIEGAGSDTIHIQG
                     VERLTGATYDVMPDRIESGTYLVAAAITGGRVRLTHAKPEHLDAVLQKLSEAGATIAT
                     GADWIELDMHGRRPRAVDIKTAPYPAFPTDMQAQFCALNTVAEGTATITETIFENRFQ
                     HVLELQRLGANLSIQGNTVIARGVERLTGAPVMATDLRASASLVIAGLAAEGTTLVDR
                     IYHVDRGYERIEAKLGRLGADIRRVAR"
     gene            complement(6398..6640)
                     /locus_tag="VM692_03435"
     CDS             complement(6398..6640)
                     /locus_tag="VM692_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HUQ51247.1"
                     /translation="MDPRDIANLIEQHLPGAKAHVQTDGQGHYEATVICAAFAGKRSL
                     QRHQMVYGTLGTRVGREIHALALETLTPEEWQARSA"
     gene            6764..7321
                     /gene="lptC"
                     /locus_tag="VM692_03440"
     CDS             6764..7321
                     /gene="lptC"
                     /locus_tag="VM692_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HUQ51248.1"
                     /translation="MRNTVVMLVLAILAAATWVATWPPQNAGLPKERSANEGPLGYYL
                     RGARLFGTDDEGRVTYRVRAERLDEVPDEARLRLEGVAVEYQPPDNTAWAISAATGSV
                     PKDGSLLDLRGNVEIRSTPSDGSNPITINTEQLRFSPESSSAESDERVRVRVGDWHFE
                     AVGLSTHLKGDALELESQVHGTFSR"
     gene            7302..>7610
                     /locus_tag="VM692_03445"
     CDS             7302..>7610
                     /locus_tag="VM692_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51249.1"
                     /translation="MARSLASSLSVGALTLVVALAAGAQRSSRGTDELVFESESMSFD
                     LQNNLIQARRPSIRQGDLHIVADEAVATGIEVDKASEWRLTGGVRIEVGTAVLEAASA
                     V"
BASE COUNT         1373 a         2451 c         2506 g         1280 t
ORIGIN      
        1 gactcaggct cgtcatcgcg aaactcggac ggtagcgcgc gcggcgccac gctcgtgcgt
       61 cgaacggctc tcccgagaca cgccggaaga ccgtccctgg ggctcgtcgg cgcacatccc
      121 tgtgcgccga cggtctcgac cgggcatgag cacacggagc acaggttttc tatttgatga
      181 ctttgagctt ggggcgcttg tcggccggcg cgggggatgg ggccggcgcc ggtgcatcgc
      241 cgtcgggcgg cgtggtcgga gcggagtcgt cgtcgccgaa gatcatgccc tgcccggtct
      301 cgcgcgcgta gatgccgagc acggctgcga tcgggaccgc gagctgctgc gatactccgc
      361 caaagcgcgc ctcgaagctg acgaactcgt tgccgagcga gagcgcgcgc gtcgcgctgg
      421 ggctgacgtt caagacgatt ttgccgtcct tgacgtgcgc ttccggcact cggacgcctt
      481 cgcgtttggc gtcgacgacc agatgcggcg tgaagccgtt gtcgagcatc cattcgtgca
      541 tcgcccgtag cagatacggg cgctgcttgg tcggggggtt ggctactgcc gtagctcccg
      601 ctccatgtcg gtgaggctcg cttggaacga cggccgcgag aacacgtcgt ccatgtagcg
      661 ctgaatcggc cgcgcctgcg gcggaagctt gatgccgaac accggcagcc gccataggat
      721 cggcgcgatc gtcgcgtcga cgagcgaaaa ctcgtcgctc aggaagaact tcttcgcggc
      781 gaagatctcg acgctcgaca ggatgctttc ctcgagcgcc ttcacggcgc gcgtggtcgg
      841 cttgcgctcg agcccgccga cgatctcacc agcgagcgaa taccagtcgt gctcgatgcg
      901 gaacagcgcg agccggaact gcgcgcgcgt cacgggatcg acgggcataa gcggcgggtg
      961 cggaaagcgc tcgtcgaggt attccatgat gacacgcgag tcgtacagca cgaggtctct
     1021 gtcgacgagc gtcggcacgc tgttgtaagg attgaggtcg agcagatcct ccggcaggtt
     1081 gccgtcgtcg acgtcgacga tgtcgatcgt gatgctcttc tcggccaaca ccaaccgcgt
     1141 ccggtgactc cacggatcgg tgggtttcga gaacaaagtc atcactgatc gacggttggc
     1201 aatgaccgtc atgcgacggg tctccttaag cttgcgaagg cggaatccgg aagaactagt
     1261 gaacgtcctt ccagatctcc ttcttcagca tataggcaat caggaggaac acgagcaaga
     1321 agccgatcac gcgaatgcca agctgctgtc gttcccgttt gatgggctcg ccgatatagt
     1381 cgaggaagtt gacgatgtcg cgcacgacgt tgtcgaactc ttcgacgccg agctcgccgg
     1441 gccgggccac gtcgaatcgc tcgaacacct cgaggccgtg ctcgtcggcc ttgaacactc
     1501 cgcgttggat accctgcagt tgccagagca catgcggcat cgcggtgccc ggcagcacga
     1561 cgttgtcgac gcccgtcggg cgagacgggt cggcatagaa actgcgcagg aacgtataga
     1621 tgtagtcggg gccgcggctg cgcgcgatca acgacaaatc cggcggcgat ttgccgaacc
     1681 accgcgccga atcctcgggg cgcatcgcgt tcgccatcgt gtcgaacggc cgctcgcccg
     1741 tgaacatcag gttgtcgatg agctgctgat cgctcagctg caaatcctga ccgaggcggt
     1801 tgtagcgcac gtacttggcg gagtggcagc cgaggcagta gttcacgaag tattttgccc
     1861 cgcgctgcaa tgacgccgtg ttagcgacgt tgttgttggc cggttcgagc tcggcctcgt
     1921 gctgcgcggc gacgggcccg gcgagagcga caccgaaaac gacggcgatg acgaagcttc
     1981 tcaccacgtg accctcgtcg gcaccggctt ggttttctcg ttgcgcgtgt agaacggcaa
     2041 caggatgaag aaagagaagt agaggatcgt aaagatcagc gacaacgtcg gagtgttgcc
     2101 gacggccggc tgcaacccga gatagccgag cgtcacgaac gtgatcgcga accccatcag
     2161 cgcgagcttg tagcccaaac tgcgataacg gatcgacttc acgcggcagc ggtcgagcca
     2221 cggcaggaaa aacagcagca ggacggctga gcccatcagc gcgacgccga gcagcttgtt
     2281 cgggacggca cgcaagatcg cgtagaacgg cgtgaagtac cagaccggtg cgatgtgctc
     2341 cggcgtggcc agcgtgttgg cgggctcgaa gttggcgtgc tcgaggaagt agccgcccat
     2401 gtcgggcgcg aagaacacga ccgccgagaa caagatcaag aaaatgatga tgatcaccaa
     2461 gtccttgctc gtgtggtacg gatggaacgc cacaccgtcg atcggatggc cgtcggcgcc
     2521 tttgtgctcc ttgatctcga tgccgtcggg attgttcgac ccgacctcgt gcaacgccat
     2581 gatgtgcacg acgacgagca gcacgagcat caacggcacg gcgatgacgt gaaacgcgaa
     2641 gaagcggttc agcgtgatgt cggagatgta gaagtcgccg cgaatccact ccgagaggcc
     2701 gttgccgatg accgggatcg cgccgaacaa cgacacgatg acctgcgcgc cccaatacga
     2761 catgttgccc cacggcagca ggtagccgaa gaacgcttcg gccatcaggc tcaggtagat
     2821 gaacatgccg atgatccaga cgagctcacg cggcttcttg taggagccgt agagcaggcc
     2881 gcggaacatg tgcaaataaa cgacgatgaa gaacgcggag gcgcccgtcg agtgcaggta
     2941 gcgaatgagc caaccccatt cgacgtcgcg catgatgtac tcgaccgacg cgaacgcatc
     3001 cgccgcggcc ggcttgtagt tcatcgtgag gaagatgccc gtgacgagct ggagcacgag
     3061 cacgaacgtg gcgatgacac cgaagccata ccagaagttg aagttcttcg acgcgtagta
     3121 ctccgtcgcg tgctccctga tgagcttcgt catcgggaag cgatcgtcga tccacgtgcc
     3181 gagcgcgctc aatcctcggc ctagccgatc aaagacatcc gcttcggtgc tcgccattta
     3241 tgagttccct gtgtcggaac cgatcagaat cgtgttgtcg ttgatgtagc ggtacggcgg
     3301 cacggggaga ttggtcggcg ccggaacgcc gtcgaacacg cggccggcga gatcgaactt
     3361 cgagccgtga cagggacagt agaaaccacc cggccactcg ggcccgagat cggccggcgc
     3421 cacctcgaag cgcggcatcg gcgcacagcc caagtgggtg cacacgccct cgagcacgag
     3481 gaacgccggg cgaacgctgc ggtgctcgtt cgtcgcgtag ctcggctgga ccgactcctc
     3541 ggactccgga tccttgagat gaccggcgtc cttcgcgagc gaggtcaaca tctcgtcggt
     3601 gcggtgcacg atgtagatcg cgcggccgcg ccactcgacg cgcagcaacg cgccgctctc
     3661 gagcttgctg atgtcgacgg tgacgggcgc gcccaacgcc tgggctcggg cgctcggctt
     3721 gaacgaggag acgaacggga cggcagtcgc gattgcgccg gctccgcctg tcacgacggt
     3781 ggcgaccgtc aaaaagtgtc gccgggtgaa accggtgctt tcagccacaa aacacccctt
     3841 gaccgacggg gtcaagcatc agaaggctcc ctcaaaatca ttgaattcca gttccgcacg
     3901 ggtgtcgcac gtgttccttt cgtacgccgc gcggccgggt tttagcggtg ccggcattat
     3961 acacagcggc caaacggctt ggccaccgag cggccgtggg ctacctgcta gactgcgcgc
     4021 catgccgccg gtcctgaagg tcgccgcctt catcgcacaa tccgttgcga tcggtctcgc
     4081 tctcgcattc atcgcggtgg tcgtcaaacc cgacctcatc aatcgcgccc cgacgaccag
     4141 cacgcagtgg agctatgcgc aggccgttgc cgcgagcgcc gcagccgtag tcaacatcaa
     4201 tacgtcgcgc gtgttcaatc tgcccaacaa cagaaggaac ttgcgggtca ggcgcgaggg
     4261 gctcggctcc ggcgtcgtca tcgaccgcga gggatacgtc gtcaccaact accacgtgat
     4321 ccaaggcgcc gacgagatcg gcgtgcagtt ggcggacggc cgcgtcgccg tgccgactct
     4381 ggtcggcacc gatcccgaca ccgagctcgc gctgctgaag atcgatctgc ccgacctgcc
     4441 cgagatccgc ctcggccgct ccgatacgct gaaggtcggc gacgtcgtgc tggcgatcgg
     4501 caactcgctc ggcctgagtc agagcgtcac aatgggcatc gtcagcgcga cgggacgcgg
     4561 tcagctcgtc gggatccctt tcgaggactt catccaaacg gacgccgcta tcaacgccgg
     4621 caactccggc ggagcgctag tcaacgcgcg aggggagctc gtcggcatca acaccgccgt
     4681 gctcagcgcg gccgacggcc aggacacacc cgaagggctc agcttcgcga tccctgtgaa
     4741 cctcgtgcgc ggcgtcatgg agcagctgat cactcacggg cgcgtgattc gcggctacct
     4801 cggagtcgac gaggccgtcg acttcccggc gagtcaggcc aagcagctcg gcatcgaagg
     4861 gtcggccgtg caaataacgc gcgccagtgg gccggcagcc gcggcgggct tgcgtgccga
     4921 cgacgttctt actcacctga acggcgagcg catgtactcc ggccagcaag ccttgaattt
     4981 cgtcgccagc ttgcagcccg gcgaccgcct cgagatccgc ggcacacgcg ccgacggcac
     5041 gccgttcacg acggaggcgg tgctcgagga gcggccgacg cccggcctca actgatcgtc
     5101 agcgcgcgac gcggcgaatg tcggcgccca accgcccgag cttcgcctcg atgcgctcgt
     5161 agccgcgatc gacgtggtag atgcggtcga cgagcgtcgt gccttccgcg gcgagccccg
     5221 caatcaccag cgacgccgac gctcgtagat cggtcgccat cacgggtgcg cccgtcagcc
     5281 tctcgacacc gcgcgcgatg accgtgttgc cttgaatcga cagattcgcg ccgagccgtt
     5341 gcagctcgag cacgtgctga aaccggttct cgaagatcgt ctccgtgatc gtcgccgtgc
     5401 cttcggcgac cgtgttcaac gcgcaaaact gcgcctgcat atccgtcggg aacgccgggt
     5461 acggcgcggt cttgatgtcg acggcgcgcg gccggcggcc gtgcatgtcg agctcgatcc
     5521 aatcagcgcc cgtggcgatc gtcgcgccgg cctcgctgag cttttgcagc accgcgtcga
     5581 ggtgctcggg tttggcatgc gtgagccgaa cgcggccacc ggtgatggca gccgcgacga
     5641 gataggtacc gctctcgatg cggtccggca tgacgtcgta ggtcgcgccc gtcaggcgtt
     5701 cgactccttg tatatggatc gtgtccgagc ccgcgccttc gatcttggcg cccatcgcca
     5761 tgaggaacgc cgcgagctcg ccgatctcgg gctcgcgagc ggcgttctcg atgacggtct
     5821 cgccggcggc acaaaccgcc gccatcatca ggttctcggt accggtcacg gtcacggtat
     5881 cgagcacgag ccgcgcgcct ttgagcttcg tcgcgcgcgc gcggatgtaa ccgttgtcga
     5941 tcgagatcga cgcgcccatg gcgcgcaggc ccatgacgtg cagattgacg ggccgcgcgc
     6001 cgattgcgca gccgcccggc aacgacacgt cggcatggcc gaatcggccg agcaacgggc
     6061 cgagcaccaa gatcgacgca cgcatcgtct tgacgagctc gtacggcgcg aggaattcct
     6121 tgatctcgcc ggagtcgacg acgacgctcg agtcgtcgcg atgctcggcc gcgacaccca
     6181 tgcgcttcag cagcttcagc gtcgtcgcga tgtcgttgag ccgcggaacg ttgccgagcg
     6241 tgacggcccc atcggcgagc agcgtagcgg cgagaatcgg cagcgcggcg ttcttcgcgc
     6301 cggagactcg aacgtcgccg tcgagcgccc tgccccctac gatcgccagc ttgtccatgg
     6361 cgccactagc cgcccgttgc ctgcgcgcgc gagctcgtca tgccgaccgg gcctgccact
     6421 cttcgggcgt cagcgtctcc agtgcaagcg catgaatctc gcggccgacg cgcgtgccga
     6481 gcgtgccgta caccatctga tgtcgctgca agctgcgctt gcccgcgaac gcggcgcaga
     6541 tgacggtggc ttcgtaatgg ccttggccgt cggtctgcac gtgcgctttc gcacccggca
     6601 aatgctgctc gatcaagtta gcgatgtcgc gcgggtccat gttcttcggc cgggtacggg
     6661 aaccgtagtc tagcgtacgg accgcgagcg ttgcgccgct cgcggagcgt ccatcgcgta
     6721 caatcgaacg tcggcgggtg ctgccgccgc gcctgaggat ctcgtgcgca acaccgtcgt
     6781 catgctcgtg ctcgcgatcc tcgccgccgc aacctgggtt gcgacctggc cgccgcaaaa
     6841 tgcgggcctg ccgaaggagc gcagcgcgaa cgagggcccg ctcggctact accttcgcgg
     6901 cgcccgcctg ttcggcaccg atgatgaggg gcgcgtgacc taccgtgtcc gggcagagcg
     6961 gctcgacgag gtgcccgacg aagcgcgcct gcggctcgag ggtgtcgccg tcgagtatca
     7021 gccgccggac aacacggcgt gggcgatctc cgcggccacg ggcagcgtgc cgaaggacgg
     7081 atcgttgctt gacttgaggg gcaacgtcga aattcgcagc acgccttcgg acggctcgaa
     7141 tccgatcacg atcaataccg agcagctgcg cttttcgccg gagagctcga gcgccgagtc
     7201 cgacgagcgc gttcgagtcc gcgtcggcga ctggcatttc gaggccgtgg gcctgagcac
     7261 gcacttgaag ggggacgctc tggagctaga atcccaggta catggcacgt tctctcgcta
     7321 gcagcctctc ggtcggcgcg ctgacgctcg tcgtcgcgct cgcggccggc gcccagcgca
     7381 gctcacgcgg caccgacgag ctcgtgttcg agtccgagtc gatgagcttc gacttgcaga
     7441 acaacctgat tcaggcgcgc cggcccagca tccggcaggg cgacctgcac atcgtggccg
     7501 acgaggccgt ggcgacgggg atcgaggtcg acaaagccag cgagtggcgc ctcacgggcg
     7561 gcgtgcgcat cgaagtcgga acggccgttc tcgaggctgc ctcggcggtc
//
LOCUS       DATLEE010000159         2866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5388715, whole genome shotgun sequence.
ACCESSION   DATLEE010000159 DATLEE010000000
VERSION     DATLEE010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5388715"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..2648)
                     /locus_tag="VM692_03450"
     CDS             complement(39..2648)
                     /locus_tag="VM692_03450"
                     /inference="COORDINATES: protein motif:HMM:NF028537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HUQ51250.1"
                     /translation="MSTLEEPGTADRRQPAPSLTQFLGPRYWPTWLLVGWLHLLARLP
                     WRGAIRVSRGVGRALWYLLPGRRRVVLRNLEICFPELGAAQRHALGRGSFENVAASVA
                     EIAIAWFGTSLPPARIEGREHLEAALGRGKGVILFSGHFTTLELTAQFITPMIPSFAF
                     MFRTRHNPLLDAIQARGRRRTSHLSFSNSDSRAMLRALRDNAVVWYAPDQAYGGPGAE
                     LLPFFGEPAMTNTATTRLARLSGSVVLPFQFRRLDDDSGYSVGFEPPVEDVPSDDAIA
                     DTKRLTGILERFVRACPQQYLWTHRRFRSRPNLPNGYARPVAQEGAPRTRRHSVLAAP
                     ALIVAVALFIVGADNDTFWQGAWRATLNDEHRLAILWSLFALVFCTLTTLLSLALGTK
                     LLRVCSALLLLVAASCGYFMAQFGVVIDESMIRNAAETTVLEATPLLSAAYFWHLALY
                     GVLPALLVLFVPLGRLRWQAAVAARAVTAAVALATLFATLYVNYAAVSFFGRDNDDLR
                     LQINPVYPLYAAIKFGFGSDDSAPQERRPLDLRSAAVPEKGKPALVVLVMGETARADR
                     FSLNGYERDTNRYTKARDVVNFPRVVSCGTSTAESVPCIFSGLGQKGFSHAAAAAHES
                     LFGALQRLGVSTFWRDNSTGCKDVCDDQHFEQRANWTDAELCDATGCFDELLLQNFDQ
                     LLAERTQDHFIVLHQRGSHGPAYYTDVPQWAKEYFPECNLASLRNCDRAAINNSYDNT
                     ILYTDYFLSRVIDELEKRSDEFTTAMLYVSDHGESLGENGLYLHGFPYAMAPREQIQV
                     PMLLWASPSFYAQRAHVDPQCVRRSAQHDTSHDAIFHTLLPLFGVESPLYDEKLDLLA
                     GCRENHLSAVTAR"
BASE COUNT          541 a          875 c          977 g          473 t
ORIGIN      
        1 ccgccgtaat agtcgagcat cgtccgcgct ctcggggctc agcgcgcggt gaccgcggag
       61 aggtggttct cgcggcaccc cgccaatagg tccagcttct cgtcgtagag cggtgactcc
      121 acgccgaaga gcggcagcaa cgtgtgaaag atcgcgtcgt ggctcgtgtc gtgctgcgcc
      181 gaccggcgta cgcactgcgg atccacatga gcgcgctgtg catagaagct cggcgacgcc
      241 cataacagca ttggaacctg aatctgctcg cgtggagcca tcgcgtacgg gaatccgtgt
      301 aagtacaggc cgttctcgcc gagcgactcg ccatgatcgg agacgtacaa catcgccgtg
      361 gtgaactcgt cgctgcgttt ctcgagctca tcgatgaccc ggctcaggaa gtagtcggtg
      421 tagagaatcg tgttgtcgta cgagttgttg attgcagcgc gatcacagtt gcgcaggctc
      481 gcgaggttgc actccgggaa gtactccttg gcccactgcg gtacgtccgt gtagtacgca
      541 gggccgtgac tgccgcgctg gtgcaacacg atgaaatggt cctgggttcg ctcggcaagc
      601 agctggtcga agttttggag cagcaattcg tcgaaacagc ccgttgcgtc gcaaagctcg
      661 gcgtccgtcc agttagcgcg ttgctcgaaa tgctggtcgt cgcaaacgtc cttgcaccct
      721 gtgctgttgt cgcgccagaa cgtgctgacg ccgagccgct gcagtgcgcc gaataagctt
      781 tcgtgcgcgg cagctgcggc gtgggaaaac cctttctgac ccaagccgga gaagatgcac
      841 ggcacggatt ccgcggtcga cgtaccgcac gacactacgc gagggaaatt cacgacgtca
      901 cgcgccttcg tgtagcgatt ggtgtcgcgt tcgtagccgt tgagggaaaa gcggtctgca
      961 cgcgccgttt cgcccatgac gaggacgacg agcgccggct tgcctttttc aggcacagca
     1021 gcgctgcgca gatcgagggg acggcgctcc tgaggtgcac tgtcgtcgga gccaaagcca
     1081 aacttgatgg cggcgtagag tggatagact gggttgatct gcaagcgcag atcgtcgtta
     1141 tctctgccga agaacgacac ggcggcgtag ttgacgtaca gcgtcgcgaa caaagttgcg
     1201 agcgcgacgg cggccgtcac tgcgcgtgcc gcgactgccg cttgccagcg cagccgtcca
     1261 agcggtacga acaagacgag gagcgccggc aggacgccat agagcgcgag atgccagaag
     1321 tacgcggcgc tcagcagcgg tgtcgcctcg agcaccgtgg tctcggcggc gttgcggatc
     1381 atcgactcgt cgatgacgac gccgaactgg gccatgaaat atccgcaact cgctgccacg
     1441 agcaatagca gcgccgagca gactcgcaga agcttggtgc cgagggccag ggagagcagc
     1501 gtggtcagcg tgcagaacac gagcgcaaac aaggaccaca agatcgcgag ccggtgctcg
     1561 tcgttcagag tcgcgcgcca agccccttgc cagaacgtgt cgttgtccgc gcccacgatg
     1621 aacagcgcga cggcgacgat gagcgcgggc gctgcgagca cactatgccg gcgcgtccgc
     1681 ggcgcgccct cttgggcgac aggccgcgcg taaccgttgg gcaaattcgg ccggcttctg
     1741 aagcgccggt gggtccagag gtactgttga ggacacgcgc gtacgaagcg ctcgaggatg
     1801 cctgtgaggc gcttcgtgtc cgcgatcgcg tcgtcgcttg gaacgtcttc taccggcggc
     1861 tcgaacccta cgctgtatcc ggaatcgtca tcgagacggc ggaattggaa cggcagcacg
     1921 acggaaccgc tcaaccgcgc aagccgggtc gtcgccgtgt tggtcatggc gggctcgccg
     1981 aagaacggca ggagctcggc gccgggtccg ccgtaggctt gatcgggcgc ataccagacg
     2041 acggcgttgt cgcgtagcgc acgcaacatc gcgcggctgt ccgagttgct gaacgacagg
     2101 tgcgacgtcc gtcggcgacc gcgcgcctgg atggcgtcga gcaatgggtt gtgccgcgtg
     2161 cggaacataa acgcaaagga cgggatcatc ggcgtgatga actgcgccgt gagctcgagc
     2221 gtcgtgaagt gaccggagaa caaaatcacg cctttgccac gaccgagagc ggcctcgagg
     2281 tgctcgcgcc cttctatgcg cgccggcggc aacgacgttc cgaaccaagc gatcgcgatc
     2341 tcggccacgg aggccgccac gttctcgaag ctgccgcgcc ctaacgcatg ccgttgggcg
     2401 gcgccgagct cggggaagca aatctcgaga ttgcggagga cgacacggcg ccgaccgggc
     2461 agcagatacc agagcgcgcg gccgacgccg cgactgacgc gaattgcgcc gcgccagggc
     2521 agccgcgcca gcaagtgcag ccagcccacc aacagccagg tcggccagta acgcggcccg
     2581 agaaattggg taaggctcgg tgcgggttgg cggcgatcgg ccgtgcccgg ctcttcgagt
     2641 gtcgacatcg tggagtagga caccccggcg cgcggaaatc ggtcgctcgc ggtaggattc
     2701 gcgaccgaaa ccgaatcgaa cggccgtcag aggcgcccgg aaggcatgac cgaacgatga
     2761 tcggagctcg cgaggtcgcc ttgttcacgc tggtcggcat ggccgcggcg tgcctcaact
     2821 tggtcgtggt cgccgagctc gtgccgctgg gcgttgcgcc cctggc
//
LOCUS       DATLEE010000160         2710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3898162, whole genome shotgun sequence.
ACCESSION   DATLEE010000160 DATLEE010000000
VERSION     DATLEE010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3898162"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VM692_03455"
     CDS             <1..1335
                     /locus_tag="VM692_03455"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HUQ51251.1"
                     /translation="NSVLLANAQLVNEGRIVAADVWVRGDRIERIGTGPTPAGTTTID
                     LAGKHLLPGLIDDQVHCREPGLTHKATLGTESLAALCGGVTSFLDMPNTQPPTTDRAA
                     LAEKRGIAAASCHVNYGFYLGATNTNLEEIKRVTQADACGVKVFMGASTGNMLVDDPQ
                     TLEGIFAECRVQIAAHCEYSPLIWENEKRARERYGDAVPIELHPEIRSAEACYRSSSM
                     AVGLAKKHGARLHVLHLTTARELELFEPGPVAAKRITGEVCAHHLWFDESSYATLGTK
                     IKCNPAIKTAADRAALRAAVVSDRIDVIATDHAPHTLEEKGRGYFEAPSGLPLVQHSL
                     LMLLDQHAQGLFTLPKIVEKAAHNPAVLFGIADRGFIREGYYADLVAVDLSGVTRVDA
                     AQVRYKCGWSPFEGTEFRGAVALTLLNGVTVFRDGQPLAVPRAARPLEFRPR"
     gene            1383..2279
                     /locus_tag="VM692_03460"
     CDS             1383..2279
                     /locus_tag="VM692_03460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUQ51252.1"
                     /translation="MKTKRALAWLLAGAAGMLFGCRSPEEPASPSSQAAVAATAAEHA
                     HDAPTPTPAAEAAPKVPVLEQDLAYGEGAKSNLVGYLAMPQDAAEPLPGIIVIHEWWG
                     LNDNIKAMTRRLAGEGYVALAVDLYGGATASTPDAAQALMAALLAEPEAATRNLSQAY
                     DYLEKYALAPRIASIGWCLGGGWSLQTALLYPETLDAMVMYYGQVTTDRQRLATLNVP
                     VLGFFGGEDQSIPVREVLQFRTTLNELGKNAEVLVVPRADHAFANPSGGNYNAQAADE
                     AWDTTLKFLERHLKLSTPTQQQ"
     gene            complement(2473..>2710)
                     /locus_tag="VM692_03465"
     CDS             complement(2473..>2710)
                     /locus_tag="VM692_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51253.1"
                     /translation="VGLSVSLLAQHATGAEVPVVQKAPVATELVVDAQLFRNEIDAYV
                     RELNRQLRTTVNEDLRRELATKIVVASNELRTRS"
BASE COUNT          481 a          885 c          897 g          447 t
ORIGIN      
        1 aattccgtat tgttggccaa cgcgcagctc gtcaacgaag ggcgaatcgt cgccgccgac
       61 gtatgggtcc gcggcgaccg catcgagcgc atcggcacgg gaccgacgcc cgcgggcacg
      121 acgacgatcg acctcgccgg caagcatctg ctgcccggcc tcatcgacga ccaggtgcac
      181 tgccgcgagc ccggcctcac gcacaaagcc acactgggaa ccgaatcgtt ggcggcgctc
      241 tgcggcggcg tcacgagctt cctcgacatg ccgaacacgc agccgccgac gacggatcga
      301 gcggcgctcg cggaaaagcg cggcattgcc gcggccagct gtcacgtcaa ctatggcttc
      361 tacctcggcg cgacgaacac gaacctcgag gagatcaagc gagtcacgca ggccgacgcg
      421 tgcggagtca aagttttcat gggcgcgtcg acgggcaaca tgctcgtcga cgatccgcag
      481 acgctcgagg gcatattcgc ggagtgccga gtgcagatcg cggcccactg tgaatattcg
      541 ccgctgatct gggaaaacga gaagcgagcc cgcgagcgct acggcgacgc cgtcccaatc
      601 gagctgcatc ccgagattcg ctcggccgag gcgtgctatc ggtcgtcgtc gatggccgtc
      661 ggcctcgcca agaagcacgg cgcgaggctg cacgtgctgc accttacgac ggcccgcgag
      721 ctcgagctgt tcgaaccggg gccggttgcg gcgaagcgca tcacagggga agtctgcgcc
      781 caccacttgt ggttcgacga gtcgagctac gcgacgctcg gcacgaagat caagtgcaat
      841 cctgcgatca aaacggctgc cgatcgggcc gcgttgcgcg cggccgtcgt gagcgaccgc
      901 atcgacgtca tcgcgaccga ccatgcgccg cacacactcg aggagaaagg gcgcggctat
      961 ttcgaagcgc cgtcggggct gccgctcgtg cagcactcgt tgctgatgct gctcgaccaa
     1021 cacgcgcagg gcttgttcac gctgccgaag atcgtcgaga aagccgcaca caatcctgcc
     1081 gtgctcttcg ggatcgcgga ccgcggcttc attcgcgaag gctattacgc ggacctcgta
     1141 gccgttgact tgagcggtgt gacgcgcgtc gacgcggcgc aggttcgcta taaatgcggc
     1201 tggtcgccgt tcgagggcac ggaatttcgt ggcgcggtgg cactgacgct cttgaacggt
     1261 gtgacggtgt tccgtgacgg tcagccgctc gcggtgccgc gggcggcccg gccgctcgag
     1321 tttcggccgc gttagcgacg cgttgccttg cccgccgtgg gtcgatgtgc tggagttttt
     1381 ccatgaaaac caagcgtgcg ttggcatggc tgttggccgg tgccgccggg atgctgttcg
     1441 gctgtaggtc gccggaggag cccgcatcgc cgtcgtcgca ggccgccgtc gcggccacgg
     1501 ccgcggagca tgcgcatgac gcgcccacgc cgactccggc cgccgaagcc gcgcccaagg
     1561 ttcccgtgct cgagcaggac ctcgcctacg gcgaaggcgc caagagcaat ctcgtcggct
     1621 acctcgcaat gccgcaagac gcggcagagc ctttgcccgg catcatcgtg atccacgagt
     1681 ggtggggcct caacgacaac atcaaggcca tgacgcggcg cctcgccggc gagggctatg
     1741 tcgcgttggc cgtcgatctc tatggcggtg cgacggcgtc gacccccgac gccgcgcagg
     1801 cattgatggc tgcgctattg gccgagcccg aggcggcgac gcgcaatctg agccaagcct
     1861 acgattacct cgagaagtac gcgcttgcgc cgcgcatcgc cagcatcggc tggtgtctgg
     1921 gcggcggttg gtccttgcag acggcgctgc tgtatcccga gacgctcgac gccatggtca
     1981 tgtactacgg gcaggtcacg acggaccgtc agcggctcgc gacgctgaac gtgccggtgt
     2041 tgggcttctt cggcggcgag gaccaaagca ttccggtccg cgaggtgctg cagttccgca
     2101 cgacgctgaa cgagctcggc aagaacgccg aagtgttggt cgtgccgcga gccgatcacg
     2161 ccttcgccaa tccgagtggc ggcaattaca acgcgcaagc cgccgatgaa gcgtgggaca
     2221 ccacgctgaa attcttagag cggcatttga agctgagcac gccgacccag caacagtagc
     2281 gtcacggccg aagcactagg tgtcgagcga gcgcgtggcg gaaaaaatgg agccgggctg
     2341 cggaacatcc gtatccttca ttgcgaagta actagttcgc tgccggaaac ccggccccct
     2401 caaaacggcc gtccgactcg gccaacggac caccacgggg ggagcagtgg tgggccgttt
     2461 gttcgttacc gtttagctcc gggtgcgcag ctcattgctg gccaccacga tcttcgtcgc
     2521 cagctcgcgc cgcagatctt cgttcaccgt cgtacgcagt tgccgattga gctcgcggac
     2581 gtaggcgtcg atctcattcc gaaacagctg cgcgtcgacg acgagctcgg tggccaccgg
     2641 tgccttctgc accacgggga cctcggcgcc tgtcgcatgc tgtgcgagca ggctcacgga
     2701 caacccgacg
//
LOCUS       DATLEE010000161         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9776198, whole genome shotgun sequence.
ACCESSION   DATLEE010000161 DATLEE010000000
VERSION     DATLEE010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9776198"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VM692_03470"
     CDS             <1..813
                     /locus_tag="VM692_03470"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HUQ51254.1"
                     /translation="ARAALDDGTLASMLGSIDLSLLADSAPGLRGDLRVVPLAEVLQL
                     FDVQEQSGVLTVERSGARVDIYFRRGRVDQAVAEGVPEEFLLGRFVMDAELIDRAQFE
                     RFLEERDGSQRLIGAQLVKQGYLIEPDLKACLTRQTSELIYEILRWRHGRFRFAAGLE
                     LPPSVIDAALGLDVEAVLMEGYRRVDEWHLIERAIDNFDIVFLRNEDQVAQMGRGRLT
                     REELAVLELVNGKNTVKDIVRKSRMGSFEVSKMLYRLLSIKLVRRRVLPVAV"
     gene            complement(922..1128)
                     /locus_tag="VM692_03475"
     CDS             complement(922..1128)
                     /locus_tag="VM692_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51255.1"
                     /translation="MKTGCSGTICADPGNEVVTTCEMKPEYACYQNATCARQADGKCG
                     WTQTPELTSCLASPPKLDSGGAPQ"
     gene            1291..>2196
                     /locus_tag="VM692_03480"
     CDS             1291..>2196
                     /locus_tag="VM692_03480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUQ51256.1"
                     /translation="MPELVKVDRYAIQRSLGAGGIGLVYEAIDSDSGAHVAIKTLRDV
                     TPDGLYRLKREFRLLQGVEHPNVCQIYELFEHDGRWFITMELVDGTSLLEYMRGDEER
                     LRDAMAQLALALCAVHEAELVHRDLKPSNVLVTDEGRVVLLDFGFVEDTAKEASPTRS
                     QSIVGTPMYMAPEQALAADAGPAADWYSFGVILFEALTGRLPFDGETALAVMLEKQRT
                     DAPRASAIVPGVPADLDELCADLLRIDPARRPSGSSVLRRLGARSSARDSRPALASVP
                     SVSGGFVGRSSELAQLRSAFNELGEV"
BASE COUNT          342 a          698 c          738 g          418 t
ORIGIN      
        1 gcacgcgctg ccctcgacga cggcacgctt gcgagcatgc tcggctcgat cgatctgagc
       61 ctcttggccg acagcgcgcc gggtttgcgc ggcgatctgc gcgtcgtgcc gctcgccgag
      121 gtgctccagc tgttcgatgt ccaggaacaa tccggtgtgc tcaccgtcga gcgcagcggc
      181 gcgcgcgtcg acatctactt tcgtcgcggt cgcgtcgacc aggcggtcgc cgagggcgtg
      241 ccggaagagt tcctgctcgg tcgcttcgtc atggacgccg agctgatcga tcgcgcgcag
      301 ttcgaacgct tcctcgagga gcgcgacggc agccagcggc tgatcggtgc gcagctcgtc
      361 aagcaggggt atctcatcga gcccgatctc aaggcgtgcc tgacgcgcca gaccagcgag
      421 ctcatctacg agatcctgcg gtggcgtcac ggccggtttc ggttcgcggc cggcctcgag
      481 ctgccgccca gcgtcatcga tgccgcgctc ggtctcgatg tcgaggccgt gctgatggag
      541 ggctaccgcc gcgtcgacga gtggcacctg atcgagcgcg cgatcgacaa cttcgacatc
      601 gtgttcctgc gcaacgaaga tcaagtcgcg cagatgggac gcgggcgttt gacgcgcgag
      661 gagctcgccg tgctcgagct cgtgaacggt aagaacaccg tcaaagacat cgttcgcaag
      721 tcgcgaatgg gcagcttcga agtcagcaag atgctgtacc gcctgctcag catcaagctg
      781 gtccgtcgtc gcgtgctgcc ggttgcggtc tgatcatcct gcgcaacgct tcgcgttgct
      841 gcgcggtcca gcaaagctgg accttgctcg tcctgcgcaa cgcttagcgt tgctgcgcgg
      901 tccagcaaag gaccttgctc gttactgcgg tgcgccgccg ctgtcgagct tcggcggcga
      961 tgcgaggcag ctcgtcagct ccggcgtttg cgtccagccg cacttgccgt cggcttgccg
     1021 cgcgcaggtc gcgttttggt agcaggcata ctctggtttc atctcgcacg tcgtgacgac
     1081 ctcgttgcct gggtcggcgc agatcgtgcc gctgcagccg gttttcacgc aatctccgtc
     1141 ggagacggcg cccgatcctt gggatccaat gggtggcgtg ttgtcggatg acggcggctt
     1201 tgatccgccg catgcgagca gaagagctgc gagtagtgcg tagcgcatgc cgcaaccata
     1261 ccgtcacctc gtcgctgtac agtcgtggca gtgcccgaat tggtgaaggt cgataggtat
     1321 gcgatccagc gatcgctcgg ggcaggagga atcgggctcg tctacgaagc gatcgatagc
     1381 gactcgggtg cacacgttgc gatcaagacg ctgcgcgatg tcacgcccga tggactctat
     1441 cggctcaagc gcgagttccg gctgctgcag ggtgtcgagc atccgaacgt ctgccagatc
     1501 tacgagctgt tcgagcacga cggccggtgg ttcatcacga tggagctcgt cgacggcacg
     1561 agcttgctcg agtacatgcg cggtgatgaa gagcgacttc gcgatgcaat ggcgcagctc
     1621 gcactcgcgc tgtgtgcggt ccacgaggcg gagctcgtgc atcgtgatct caagccgtcg
     1681 aacgtgctcg ttaccgacga gggccgcgtc gtgttgctcg actttggatt cgtcgaggac
     1741 accgcgaagg aagcgtcgcc gacgcgcagc caatcgatcg tcggcacgcc gatgtatatg
     1801 gcgcccgagc aagcgcttgc cgccgatgct ggacccgctg ccgattggta cagcttcggc
     1861 gtcatcctgt tcgaggcgct cacggggcgc ttgccgttcg acggcgagac cgcactcgcg
     1921 gtgatgctcg agaagcagcg cacggatgcg ccgcgggcgt cagcgatcgt gccgggcgta
     1981 cccgccgacc tcgacgagct gtgcgcggac ttgctgcgaa tcgatccggc gcgccgccct
     2041 tcagggtcgt cggtgctgcg ccgcctcggc gccaggtcat ccgcgcgcga tagccgccct
     2101 gccctcgcat cggtgccatc ggtcagcggc ggcttcgtcg gtcgcagctc cgagctcgcg
     2161 cagctgagat ctgcgttcaa cgagctcggc gaagtc
//
LOCUS       DATLEE010000162         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6049424, whole genome shotgun sequence.
ACCESSION   DATLEE010000162 DATLEE010000000
VERSION     DATLEE010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6049424"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VM692_03485"
     CDS             <1..399
                     /locus_tag="VM692_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51257.1"
                     /translation="DTFLLHARRIYPIVDIFGRAQAGDLQFEAWYGQWLGKLSEADRA
                     LWQQLRDEHTRHEHDHGAALIEAEISVPADPSITASQSVPDARTDIHKRMVRFAAAPN
                     RAASDVCADYLRLAKRFVGEFVRDYARFLP"
     gene            494..883
                     /locus_tag="VM692_03490"
     CDS             494..883
                     /locus_tag="VM692_03490"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002766286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clan AA aspartic protease"
                     /protein_id="HUQ51258.1"
                     /translation="MGIVTAKLRLSNPRKPSLAPVEVGALADTGSVFLCIPHHLYLQL
                     GLEVLQEREVKLADGSCVSVPYVGPVIVEFKNRMGVMGALVLGDEVLLGAIPMEDMDL
                     VVDPRDRTVDVNPASPNIASGIAKRAA"
     gene            complement(1021..>2458)
                     /locus_tag="VM692_03495"
     CDS             complement(1021..>2458)
                     /locus_tag="VM692_03495"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HUQ51259.1"
                     /translation="FRFVNAEGESRFVKFHWSPLAGTHSLVWDEAVKLAGADSDFHRR
                     DLWEAIEAGAYPEYELSVQVFTERDAERFSFDVLDSTKLVPEELVPLRPIGRLVLNRN
                     PDNFFAETEQVAFCTAHIVPGLDFTNDPLLAGRIHSYVDTQITRLGGPNFHEIPINSP
                     VAQVHNNQRDGFHRQAIPRGRVAYEPNSLGGGCPYQAGTMGFMSFPEQTEEHKVRGKP
                     ERFAEHYNQATLFWNSQSDIEKSHIIDAFRFELTKVQTVAIRRRMVAGLRNVAEELAE
                     GVASGLGLELPEPLPKVLERAPRAEVDSSPALSLFARPGDGEIKARRIAILVADGVDG
                     EPLERLHAELAKRGAVPRFVGALLGSVQPASGEPIEVEISIDAGPAVVYDAVVLPDGK
                     DAVEELAQLGQAMEFVKDMYRHCKPILALGASGDLLGSAGIWKVLPNGEPDPGVLHFE
                     KPRGSLDAFVAAIAKHRHYERETRPPRI"
BASE COUNT          427 a          807 c          781 g          443 t
ORIGIN      
        1 gatacgttcc tgctgcacgc gcgtcgcatc tatccgattg tcgacatctt cggcagagcg
       61 caggccggcg acctgcagtt cgaggcgtgg tacggccaat ggctcggcaa gctcagcgaa
      121 gcggaccgag cgctctggca gcagctgcgc gacgagcaca cgcgtcacga gcacgatcac
      181 ggcgctgcgc tgatcgaggc ggagatctcc gtccccgctg acccttccat aacggcgagt
      241 caatccgtgc ccgacgcgcg aacggacatt cataagcgca tggtgcgctt cgccgccgcg
      301 ccgaatcgtg ccgcgagcga cgtctgcgcc gactacttgc gcttggcgaa gcgcttcgtc
      361 ggcgagttcg tccgcgatta cgcgcggttt ctgccgtgag ctgcttcgcc tgcggattac
      421 agcggcgctt ttccacttga tcccgctgta aatgcgcgtg gtagtctgcg atcaccgcct
      481 gcaaactgga ggcatgggga tcgtgactgc gaagctcagg ctgagtaacc cgcgtaagcc
      541 ttcgctggcg ccggtcgagg tcggcgccct cgccgatacc ggctcggtat tcctttgcat
      601 cccgcaccat ttgtacttgc agctcggact cgaagtgttg caggaacgcg aggtgaagct
      661 cgcggacggt tcctgcgtaa gcgttcctta cgtcggcccg gtcatcgttg agttcaaaaa
      721 cagaatgggc gtcatgggcg cgttggtact cggtgacgag gtcttgctcg gcgcgattcc
      781 gatggaggac atggacctcg tcgtcgatcc gcgcgatcgc accgtcgatg tgaatccggc
      841 gagccccaat atcgcgtcgg gcattgccaa gcgcgcggcc taaacgcgcg gcctaaaccg
      901 agccgagtgc gctcgacgca agcgacgcaa gcgacgcaag cgacgcacga acagtcgcta
      961 cggatctgaa gcggccgtga aacgcgccac gtggcgcgtc ttgaaaacct cgcgcgctcg
     1021 ctagatcctc ggcggccgcg tctctcgctc atagtgtcgg tgcttggcga tcgcggccac
     1081 gaaggcgtcg agcgagccgc ggggcttctc gaaatgcagc acgccgggat cgggctctcc
     1141 gttcggcagc accttccaga tgccggccga accgagtagg tcacccgatg cgccgagcgc
     1201 caggatcggc ttgcaatggc ggtacatgtc cttgacgaac tccatagcct ggccaagctg
     1261 cgccagctct tcgacggcat ccttgccgtc cggcaacacg acggcgtcgt agacgacggc
     1321 aggccccgcg tcgatcgaga tctcgacctc gatcggctca ccgctcgcgg gctgcaccga
     1381 gccgagcagc gctccgacga accgcggcac ggcgccgcgt ttggcaagtt ccgcatgtaa
     1441 ccgttccagc ggctcaccgt ccaccccgtc ggcgacgagg atcgcgatgc ggcgcgcctt
     1501 gatctcgccg tcacccggcc gggcgaacag cgatagcgcc ggcgaggaat cgacttccgc
     1561 acgcggcgca cgctcgagca ccttcggcaa cggctcgggc aactcgagcc cgagccccga
     1621 ggccacgcct tcggcaagct cctcggcgac gttgcgcagg ccggcgacca tgcggcggcg
     1681 gatcgcgacg gtttgcactt tcgtcagctc gaaccggaac gcgtcgatga tgtgcgactt
     1741 ttcgatgtcg ctttggctgt tccagaacaa cgtagcctgg ttgtagtgct ccgcgaacct
     1801 ttcgggctta ccgcgcacct tgtgctcttc ggtttgctca gggaacgaca tgaatcccat
     1861 cgtaccggcc tggtaggggc aaccgccgcc gagcgagttc ggctcgtacg ccacgcggcc
     1921 gcgcgggatt gcttgccgat gaaagccgtc gcgctgattg ttgtgcactt gcgcgaccgg
     1981 tgaattgatc gggatttcgt ggaaattcgg accgcccagc cgcgtgatct gcgtatccac
     2041 gtacgaatgg atccggccag cgagcagcgg gtcgttggtg aaatcgaggc cgggcacgat
     2101 gtgggccgtg caaaatgcca cctgctccgt ctccgcgaag aagttgtcgg ggttgcggtt
     2161 caggacgagc cggccgatcg gacgcagcgg cacgagctcc tccggcacga gcttcgtcga
     2221 gtcgagcacg tcgaagctga accgctcggc gtccctttcc gtgaagacct gcacgctgag
     2281 ctcgtactcc ggatacgcgc ccgcttcgat cgcttcccat agatcgcgac ggtgaaagtc
     2341 cgagtcggcg cccgcgagct tcaccgcttc atcccagacc aacgagtgcg ttcctgcgag
     2401 cggcgaccaa tggaatttaa cgaagcgcga ctcgccctcc gcgttcacga accggaac
//
LOCUS       DATLEE010000163         4693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1669706, whole genome shotgun sequence.
ACCESSION   DATLEE010000163 DATLEE010000000
VERSION     DATLEE010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669706"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VM692_03500"
     CDS             <1..520
                     /locus_tag="VM692_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HUQ51260.1"
                     /translation="YSCITGGIGALRGPKHGGANEVALEIQQRYQTPDEAVADIRARL
                     ARKEVVIGFGHPVYTVADPRNRVIKDVARRLSAEAGDDTLFRVADAIETEMAAAKKMF
                     ANLDWFSAVAYHVMGVPTVMFTPLFVIARTTGWAAHVIEQRADGKIIRPSANYVGPEN
                     RRFVPLAERAGR"
     gene            complement(582..1085)
                     /locus_tag="VM692_03505"
     CDS             complement(582..1085)
                     /locus_tag="VM692_03505"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HUQ51261.1"
                     /translation="MSETTAYEFSAREIDGRERPLAELAGRVLLVVNVASKCGFTPQY
                     RGLEALWRKHAAAGLTVLGFPCDQFGHQEPGDESAIKEFCSLNYDVTFPLFAKIEVNG
                     AGAHPFYQWLKHEQAGVLGTEAIKWNFTKFLVDRAGRVVKRYAPNVSPEAIDGDVAAL
                     LASRGAP"
     gene            complement(1166..1993)
                     /locus_tag="VM692_03510"
     CDS             complement(1166..1993)
                     /locus_tag="VM692_03510"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HUQ51262.1"
                     /translation="MSSAPRTVCFVSDRTGVTTETVGHALLSRFEGMQFDSLTMPFVL
                     SATAARSVVMQIDAIATKQHAKPIVFCTIVDEEIRGIIKQANALGVDLFSNIGELERE
                     LGAPAGVAVPAMHGATRDPTYAARISATNYALANDDGSGAQDYRAANVVLVGVSRTGK
                     TPTCLYLALQYGVLAANYPLAEEQFEAGELPRTLLLHREKLYGLTLGAERLQQIRSER
                     RPGTRYASRQQIQYELQAAQAMYDRYSLPHLDVSECSIEEIASRILDEMHLVRHARS"
     gene            complement(1995..4367)
                     /gene="ppsA"
                     /locus_tag="VM692_03515"
     CDS             complement(1995..4367)
                     /gene="ppsA"
                     /locus_tag="VM692_03515"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011333246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HUQ51263.1"
                     /translation="MPPYVLPLRDLERADVELVGGKNSSLGEMLRELGGLGVRVPDGF
                     ATTVHAYRDFLLHNGLARRIDALLDGLDVQDLHALARTGAEIRRAILETPLPSRLSEE
                     LVTAWRMLRAGPSSGVAVRSSATAEDLPDASFAGQQETFLNVRGERDLIAATHAVFAS
                     LFNDRAIAYRVHQGFDHSRVGISVGVQHMVRSDLGSSGVMFTLDTDSGFRDAVFITSS
                     YGLGETVVQGAVNPDEFYVYKPALAAGKRAILRRNLGAKAIKMVYAEGPAAGRVATVP
                     VPPAQRRSFSLTDEDVETLARHAVTIEDHYGCPMDIEWAKDGETGELYIVQARPETVQ
                     SRNAGRSLQRFELGSRSEVLAEGRSIGQKIAAGRARVVAGVDEIARVEPGDVLVTDMT
                     DPDWEPIMKRAAAIVTRRGGRTCHAAIIARELGVPAVVGCGDSLDAVDDGRPVTVSCA
                     EGEAGYVYDGALPFERREIELETLPPAPLKIMMNVANPDRAFGFAGIPNHGVGLARLE
                     FIVNRMIGVHPRALLEYASLNASLRAAIDEQMAGYSDPVEFYVTKLAEGIAQIAAAFA
                     PSPVIVRLSDFKSNEYANLLGGKEYEPREENPMLGFRGAARYVDPSFQPCFELECRAL
                     RFVREDMGLKNVEVMVPFVRTVAEAERVTKLLAANGLARGVDGLRVIMMCELPTNALL
                     AERYLEHFDGMSIGSNDMTQLTLGIDRDSGVVAASFDERDDAVKALLKMAITACRKHG
                     KYIGICGQGPSDHPDFARWLVAQGIDSLSLNPDTVVETWLMLAGRQPSRH"
BASE COUNT          800 a         1571 c         1517 g          805 t
ORIGIN      
        1 ctactcgtgc atcacgggag gaatcggcgc actgcgcggc ccgaagcacg gcggcgcgaa
       61 cgaggtcgcg ctggaaattc agcaacgcta tcaaaccccc gacgaagcgg tcgcggacat
      121 tcgcgcgcga cttgcgcgca aagaagtcgt gatcggcttt ggccatcccg tgtacacggt
      181 agccgacccg cgcaaccgcg tcatcaagga cgtcgcacga cgtttgtcgg cggaagccgg
      241 cgacgacact ttgtttcgcg tcgccgatgc gatcgagacg gagatggccg cggcgaagaa
      301 gatgttcgcg aacctcgact ggttctcggc cgtggcgtac cacgtgatgg gcgtgccgac
      361 ggtcatgttc acaccgctct tcgtgatcgc ccgcacgacg ggctgggccg cgcacgtcat
      421 cgagcaacgc gccgacggca agatcatccg gcctagcgcg aactacgtcg gccccgagaa
      481 ccgccgcttc gtaccgctcg ccgagcgcgc tggacgttag cctaccgcgc acgaagttgc
      541 agcaagagct tctccgcccc gcaagcgccg cgcgcgctga ctcaaggggc gccgcgcgac
      601 gcgagcagcg ctgcaacatc gccgtcgatc gcctcgggcg aaacgttcgg cgcataacgc
      661 ttcacgacgc gcccggcccg gtcgaccaag aacttcgtga agttccactt gatcgcctcg
      721 gtgccgagca cgcccgcctg ctcatgcttg agccattggt aaaacggatg cgcaccggcg
      781 ccgttcactt cgatcttggc gaacagcggg aacgtaacgt cgtaattcag tgagcagaat
      841 tccttgatcg cgctctcgtc gcctggctcc tggtgaccga attggtcgca cgggaaaccg
      901 agcaccgtga gccccgcagc ggcgtgcttg cgccacagcg cttcgaggcc gcgatactgc
      961 ggcgtgaagc cgcacttcga cgcgacgttc acgacgagca gcacgcgacc ggcgagctcc
     1021 gcgagcggcc gctcgcggcc gtcgatttcg cgcgcgctga actcataggc ggttgtctca
     1081 ctcatcggta acccccttcc ggccggttcg cgctcctatt ggatgccgca atcgacgccg
     1141 cgggcaagct tcggccgcgg caaggctagc tgcgcgcgtg ccgcacgagg tgcatctcgt
     1201 ccaggattct gctcgcgatc tcttcgatgc tgcattcgct gacgtcgagg tgcggtaacg
     1261 aataacggtc gtacatcgcc tgtgctgctt gcagctcgta ctggatctgc tggcgcgacg
     1321 cgtaacgggt gccgggccgc cgctcgctgc gaatttgttg taaacgctcg gccccgagcg
     1381 tgagcccgta gagcttctca cgatggagca gcagggtacg tggcagctcg ccagcttcga
     1441 attgctcttc ggcgagcgga tagttcgcag ccaagacgcc gtactgcagc gcgagataga
     1501 ggcaggtcgg cgtcttgccg gtgcgcgaca caccgacgag cacgacgttg gccgcgcggt
     1561 agtcttgggc cccggagcca tcgtcgttcg cgagcgcgta attcgtcgcg ctaattcgcg
     1621 ccgcgtaggt cggatcccgc gtcgcgccgt gcatcgccgg cacggcgacg ccggctggcg
     1681 cgccgagctc gcgctcgagc tcgccgatgt tgctgaagag gtccacgccg agcgcattcg
     1741 cctgcttgat gatgccgcga atctcctcgt cgacgatcgt gcagaacacg atcggcttcg
     1801 cgtgctgctt cgtcgcgatc gcgtctatct gcatgacgac ggagcgcgcg gccgtcgcgc
     1861 tcagcacaaa cggcatcgtc agcgagtcga attgcatgcc ttcgaatcgc gacagcaagg
     1921 catgccctac cgtttccgtc gtcacgccgg tgcggtccga gacgaagcag accgtgcgcg
     1981 gcgcgctgct catgtcagtg ccgcgacggt tgccgcccag cgagcatcaa ccaagtttcg
     2041 acgacggtgt cggggttcaa tgaaaggctg tcgatgccct gcgcgacgag ccagcgcgcg
     2101 aagtcgggat gatccgacgg cccttggccg cagatcccga tgtacttgcc gtgcttgcgg
     2161 caagcagtaa tggccatctt cagcaatgcc ttcacggcat cgtcgcgctc gtcgaacgac
     2221 gcggcaacca cacctgagtc gcgatcgatc ccgagtgtga gctgcgtcat gtcgttagag
     2281 ccgatcgaca tgccgtcgaa gtgctcgaga tagcgctccg cgagcagtgc gttcgtcggc
     2341 agctcgcaca tcatgatgac gcgcaagccg tcgacgccgc gcgcgaggcc gttggcggcc
     2401 aacagcttcg tcacgcgctc cgcctcggcg accgtgcgca cgaacggcac catgacttcg
     2461 acgttcttga gtcccatatc ctcgcgaacg aagcgcagcg cacggcattc gagctcgaag
     2521 catggctgga acgacggatc gacatagcgc gcggcgccgc gaaatcccag catcgggttt
     2581 tcttcgcgcg gctcgtattc cttgccgccg agcaagttcg cgtactcgtt cgacttgaag
     2641 tccgagagcc gcacgatgac gggggacggc gcgaacgcgg ctgcaatctg cgcgatgccc
     2701 tcggccagct tcgtcacgta gaactcgacg ggatcggagt agcccgccat ctgctcgtcg
     2761 attgcggcgc gcaggctcgc attgagactt gcatactcga gcagcgctcg cgggtgcacg
     2821 ccgatcatgc ggttgacgat gaactcgagc cgcgccagac cgacgccatg gttcggaatt
     2881 cccgcaaagc cgaacgcccg atcggggttc gccacgttca tcatgatctt cagcggagcc
     2941 ggcggcaggg tctcgagctc gatttcgcgg cgctcgaaag gtagggctcc atcgtagacg
     3001 tagcccgcct cgccctcggc gcacgagacc gtgacgggcc ggccgtcatc gacggcgtcg
     3061 agactatcgc cgcaaccgac gaccgctgga acaccgagct cgcgcgcgat gatggccgcg
     3121 tggcacgttc gcccgccgcg ccgcgtgacg atcgccgccg cacgcttcat gatgggttcc
     3181 cagtcgggat ccgtcatgtc ggttacgagc acgtcgccgg gttcgacgcg cgcgatctcg
     3241 tccacgccgg caacgacgcg cgcgcggcct gccgcgatct tttgcccgat gctgcggcct
     3301 tccgcgagca cttccgagcg cgagccgagc tcgaagcgct gcaggctgcg gccggcattg
     3361 cggctttgca ccgtctcggg acgcgcctgc acgatataga gctcgccggt ctcgccgtcc
     3421 ttggcccact cgatgtccat cgggcaaccg tagtggtcct cgatagtgac cgcgtggcgc
     3481 gccagagtct cgacgtcttc atccgtcagt gagaacgagc gccgctgcgc cgggggcacc
     3541 ggcactgtcg cgacgcggcc cgcggccggg ccttcggcgt acaccatctt gatggcttta
     3601 gcaccgaggt tgcgccgcag gatcgcacgc ttgcctgcgg cgagcgccgg cttgtagacg
     3661 tagaactcgt cgggattcac ggcgccttgc acgaccgtct cgccaaggcc gtacgagctc
     3721 gtgatgaaca ccgcgtcgcg gaagcccgag tcggtatcga gcgtgaacat gacgccgctc
     3781 gagcccagat cgctgcggac catgtgctgc acgccgaccg agatgccgac tcgcgagtga
     3841 tcgaagccct gatgcacgcg ataggcgatg gcgcgatcgt tgaacagcga ggcgaacacg
     3901 gcgtgtgtcg cggcgatcag gtcgcgctcg ccgcggacgt tgagaaacgt ctcctgctgt
     3961 ccggcgaacg atgcgtcggg caggtcctcg gccgtcgccg aagagcgtac ggcgacgccg
     4021 gaactcgggc ccgcgcggag cattcgccaa gccgtgacca gctcttcgct caatcgcgat
     4081 ggcaacggcg tctcgaggat cgcgcggcgg atctcggcgc ccgtccgcgc gagcgcgtgc
     4141 agatcttgca cgtcgagacc gtcgagcagc gcgtcgatgc ggcgcgcgag gccgttatgc
     4201 agcaggaaat cgcgataagc gtgcaccgtg gtcgcgaacc catccggcac ccggacgcct
     4261 aggccgccga gctcccgcag catctcccct aacgacgagt ttttgccgcc gacgagctct
     4321 acgtcggcgc gctccaagtc gcgcaaagga aggacgtaag ggggaatctc ctgcggggga
     4381 gcggtagttt gaaaacgagc cgcttcgttc tgccgtttca cgccgttgcc tcgccaaacc
     4441 gcgatagctt gtacgagcaa gttccgtgcc cgacggcggc gcccgacgag gcgctgctgc
     4501 gtcaggacgg ccgacgcaag atccgcaata ccgtgttgaa ttcgcgatcg cgccgagcgc
     4561 ggcgcgacac gttatatctt tgcgcttcgc gagcacctcg gactcggcgt acaccgtgtc
     4621 atcttggttt ctccgccgcg gccgccagct ccgcaagcac gtgattggta tgctcgccca
     4681 gcgccggttc acg
//
LOCUS       DATLEE010000164         5657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6797685, whole genome shotgun sequence.
ACCESSION   DATLEE010000164 DATLEE010000000
VERSION     DATLEE010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6797685"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VM692_03520"
     CDS             <1..846
                     /locus_tag="VM692_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HUQ51264.1"
                     /translation="GALNRLLQLRPDERRAGVVAFSSGNHAQAVALAARWLEMPATIV
                     MPADAPKVKLERTRDYGAEVVLYDREREDREAIALGLAQQRGAAVVPPFDHPDIIAGQ
                     GTVGLELGRATLARQLRVDALYVPVSGGGLIAGCALGIKSQWPKCSVYGVEPRGFEDL
                     AASLAAGERRTFEPGGTTLCDGLRAPLPGAITFAINRSELAGACAVDDTQVRGAMALA
                     AEHLKVVVEPSGAAAFAAVLATPHAAGACIAVVLSGGNVDTELLTDVLRAHGAKKMTP
                     PNSHK"
     gene            889..4392
                     /locus_tag="VM692_03525"
     CDS             889..4392
                     /locus_tag="VM692_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HUQ51265.1"
                     /translation="MQLRQIATLDDWYRFDDAGPLFITGLQALVRLLLAQSHRDRLAG
                     LDTAGFVSGYRGSPLGGFDRELWRAGKYLDAAQIRFQPGLNEDLAATSIWGAQQANLF
                     EGAKHVGVFGMWYGKGPGVDRSGDPFKHGNAAGTSRHGGVLAVAGDDHTCKSSSLPHQ
                     SEYAFIDASMPVLNPSNVEEIVALGLYGYALSRFSGCWVGLKVTQETADATQSFALLP
                     QALEIVTPEFALPPGGLNIRWPDPPNDQEFRLQRYKLPAALAFARANSLDRTIIDSRY
                     PRLGIVTTGKAHLDVLQALDDLGIDRARAADVGIALFKVGMSWPLEPQAIRQFAEGLE
                     EIVVIEEKRGLVESQLKEQLYNWNAQRRPLIIGKQDERGEWLLPSNGELTPALIARVL
                     AKRMRRFHSSTDIEERVRFLEQQDRRLAAVGNDVQRLPHFCSGCPHNTSTRVPEGSRA
                     VGGIGCHYMAAWMDRDTVTFTQMGGEGATWIGQAPFTETQHVFQNLGDGTYAHSGALA
                     IRAAVAAGVNMTYKILFNDAVAMTGGQPVEGHLTVAGVAQQLVGEGVKPIIVVTDEPQ
                     KYAGPNGLPPSVTVHHRRELERLQRELRDIKGVSALIYDQTCAAELHRKRKRNLVPTP
                     DRRVVINELVCEGCGDCSVQSNCLSVMALETEFGRKRRINQSSCNQDFSCLNGFCPSF
                     VTLKGAKRNPPRPLAATALPSLPEPSQAALDGVHNVLIAGVGGTGVVTASGLLGLAAH
                     LEGKAVQELDQTGLAQKYGAVLSHVRIAADAERLHGMRIPAGQVDLLLGADLIVAAGK
                     EPLAMLSADRSAVIVNTHEEMPSDFIRDRDFAFPGKALLGALRSAGRAGAVATLDATR
                     LASALLGDSIGANVLMLGFAFQRGLLPVSGAALYRALELYGRNVGENKLAFDWGRFAA
                     ESLAQVERLASAREGEQPTALSLPETIARRDEFLGAYQDRAYAERYRARLARVAAAEQ
                     RARPGSQALQEAVARNYFAVLAYKDEYEVARLHTQSGFVDSVKRNFGTGVRMSFHFSP
                     PLFARRDAATGRPKKYELGPWVLPILRVLAKLRWLRGSKLDPFGRSADRRLERELVAR
                     YEALLDRIVAELDDSRCELALALARLPEQVRGYGPIKAAAAARATTAEQELWQQWAAP
                     AARADRARASAAREPARAAAR"
     gene            complement(4393..4737)
                     /locus_tag="VM692_03530"
     CDS             complement(4393..4737)
                     /locus_tag="VM692_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020584910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HUQ51266.1"
                     /translation="MDQYARIREQLLAKQGELARRLERLKENLTGGRSADSQEQAQEL
                     ENAEVVDALGNEARSEISKIAKALDQIKNQTYGICADCGETIPMARLEAYPFADRCIR
                     CAKAAESGRRLR"
     gene            4870..5565
                     /locus_tag="VM692_03535"
     CDS             4870..5565
                     /locus_tag="VM692_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUQ51267.1"
                     /translation="MRLLAGTSGFSYKEWVGEFYPEKTPAKSMLAYYATRLPIVEINN
                     TFYRMPNPEAIQGWRAQVPDSFSFAIKVPQRISHFKRLRDCGADLRVLLAALEDLGGS
                     LGSLLVQLPPHARCDTAALESFLSELPPGARAAFEFRHDSWIVPRVFELLQARNAALV
                     QSESDDKFEPLPWTADWAYLRLRKVDYTHADLVRWHALIEAAGVTEAQVFFKHEDGAT
                     GPKLARQLLALSN"
BASE COUNT          897 a         1876 c         1937 g          947 t
ORIGIN      
        1 ggcgcgctga atcgcctcct gcagctccgc cccgacgagc gccgtgccgg agtcgttgca
       61 ttctcgtccg gcaaccatgc gcaagccgtc gcgttggccg cgcgctggct cgagatgccc
      121 gcgacgatcg tcatgccggc cgatgcgccg aaggtaaagc tggagcggac gcgcgactat
      181 ggcgccgagg tcgtcctcta cgatcgcgag cgcgaggacc gcgaggcgat cgccctaggt
      241 ctagcccagc agcgcggcgc cgccgtcgtg ccgccgttcg accatccgga catcatcgca
      301 gggcagggaa cggtcgggct cgagctcggc cgcgcgacgc tcgcgcgaca gttgcgcgtc
      361 gacgcgctct acgtgcccgt cagcggcggc gggctcattg ccggttgcgc gctcggtatc
      421 aagtcgcagt ggccgaaatg ctccgtgtac ggcgtcgagc cgcgcggctt cgaggacttg
      481 gccgcatcgc tggctgccgg cgagcggcgc acgttcgagc ccggcggcac gacgctatgc
      541 gacgggctgc gcgcgccgtt gccgggcgcg atcacgttcg caatcaatcg tagcgagctg
      601 gcgggcgcgt gcgccgtcga cgacacgcag gtccgcggcg cgatggcgct cgcagccgag
      661 catctcaaag tcgtcgtcga gccgtccggc gctgcagcgt tcgccgccgt gctcgcgacg
      721 ccgcacgccg cgggcgcatg catcgcggtc gtgctgtccg gcggcaacgt ggatacggag
      781 cttttgaccg acgtgctgcg agcgcacggc gccaagaaaa tgacgccgcc caacagtcat
      841 aaatagccca taatgccggg cgaccctggg agcagggcag ggagaggcat gcagctcagg
      901 cagatcgcaa cactggacga ttggtaccgg ttcgacgacg ccggaccgtt gttcatcacg
      961 ggtctgcaag ctttggtccg cctgctgctc gcgcaaagcc accgcgatcg gctagccggg
     1021 ctcgacacgg cgggtttcgt ctcgggctat cgcggctcgc cgctcggcgg cttcgatcgc
     1081 gagctgtggc gcgcgggcaa gtatctcgac gcggcgcaaa ttcgctttca gcccgggctc
     1141 aacgaagatc tcgcggcgac ctcgatttgg ggcgcgcagc aagccaacct gtttgagggc
     1201 gctaagcacg tcggcgtctt cggcatgtgg tacggcaaag gccccggcgt cgatcgttcg
     1261 ggcgatccgt tcaagcacgg caacgcggcg gggacctcgc gtcatggcgg cgtgctcgcg
     1321 gtcgccggcg acgaccacac ctgcaagtcc tcgtcgttgc cgcaccagag cgagtacgca
     1381 ttcatcgatg cgtcgatgcc ggtcctcaat ccgtcgaacg tcgaggagat cgtcgcgctc
     1441 ggcctctacg gctatgcgtt gtcgcgcttc agcggttgct gggtcgggct caaggtcacg
     1501 caggaaacgg ccgacgcgac gcaaagcttc gcgctgttgc cgcaagcgct cgagatcgtc
     1561 acgcccgagt tcgcgctgcc gcccggcggc ttgaacattc gttggcccga tccgccgaac
     1621 gatcaagagt ttcgtctgca gcgctacaag ctgcctgctg ctctcgcgtt cgcgcgcgcg
     1681 aattcgctcg atcgcacgat catcgacagc cgctatccgc ggctcggtat cgtgacaacg
     1741 ggcaaggcgc acctcgacgt gctgcaagcg ctcgacgacc ttggtatcga tcgcgctcga
     1801 gcggccgacg tcggcatcgc gctgttcaag gtcggcatga gctggccgct cgagccgcaa
     1861 gcgatccggc agttcgccga aggtctcgag gagatcgtcg tcatcgagga gaagcgcggc
     1921 ctcgtcgaga gccagctcaa agagcagctc tacaactgga acgcgcagcg gcggccgctg
     1981 atcatcggca agcaggacga gcgcggggag tggctgctgc cgtcgaacgg cgagctgacg
     2041 ccggcgttga tcgcgcgcgt gctcgcgaag cgcatgcgcc gcttccactc gagcacggac
     2101 atcgaggagc gcgtgcgctt cctcgagcag caggatcggc gactcgccgc cgtcggcaac
     2161 gacgtgcagc ggctgccgca cttctgctcg ggctgcccgc acaacacgtc gacgcgggtg
     2221 ccggaaggca gccgcgccgt cggcggcatc ggctgccact acatggcagc ctggatggat
     2281 cgcgacacgg tcacgtttac gcagatgggc ggcgagggtg cgacgtggat cggtcaagcg
     2341 ccgttcacgg agacgcagca cgtctttcag aacttgggcg acggcacgta cgcgcactcc
     2401 ggtgcgctgg cgattcgcgc ggccgtcgcg gccggcgtga acatgaccta caagatcctg
     2461 ttcaacgacg ccgtcgcaat gacgggcggg cagcccgtcg aagggcatct gacggtcgcc
     2521 ggcgtggcgc agcagctcgt cggcgaaggc gtcaagccga tcatcgtcgt cacggacgag
     2581 ccgcagaagt acgccggtcc gaacggtctg ccgccgtcgg tcacggtgca tcaccggcgc
     2641 gagctcgagc gcttgcagcg cgagctgcgc gatatcaagg gcgtgtcggc gctgatctac
     2701 gatcagactt gcgcggccga gctgcaccgt aagcgcaagc gcaacctcgt gccgacgccg
     2761 gatcggcgtg tcgtcatcaa cgagctcgtc tgcgaagggt gcggcgactg cagcgtgcag
     2821 tcgaactgct tgtccgtgat ggcgctcgag acggagttcg gccgcaagcg gcgcatcaac
     2881 caatcgtcct gcaaccagga cttcagctgc ttgaacggtt tctgtccgag cttcgtgact
     2941 ttgaaaggcg cgaagcgcaa tccgccgcgc ccgctcgcgg ctacggcttt gccgagcctg
     3001 ccggagccga gccaagcggc gctcgacggt gtgcacaacg tgttgatcgc gggcgtcggc
     3061 ggcacgggcg tcgtgacggc gagcggcttg ctcggcctcg cggcgcatct cgaaggcaag
     3121 gccgtgcaag agctcgatca gacggggctc gcacagaagt acggcgccgt gctgtcgcac
     3181 gtgcgcatcg ccgccgacgc cgagcgtttg cacggcatgc ggatcccggc cggccaggtc
     3241 gatctgttgc tcggcgcgga cctcatcgtc gcggccggca aggagccgct ggcgatgctg
     3301 tcggcggatc gctcggccgt gatcgtcaat acgcacgagg aaatgccgtc ggatttcatc
     3361 cgcgatcgcg acttcgcgtt tccgggcaag gcgctgctcg gagcgctgcg ctccgcgggt
     3421 cgcgcgggcg ccgtcgccac gctcgacgcc acgcggctcg cgtctgcgct gctcggcgac
     3481 agcatcggcg cgaacgtgct gatgctcggg ttcgcgtttc agcgtggtct gctgccggta
     3541 tccggcgcgg cgctatatcg cgcgctcgag ctctacggcc gcaacgtcgg tgagaacaag
     3601 ctggcgttcg actggggccg tttcgcggcc gagagcctcg cgcaagtcga gcggctcgcg
     3661 tcggcgcggg aaggcgagca gccgaccgcg ttgtcgctgc cggagacgat cgcccggcgc
     3721 gacgagtttc tcggcgcgta tcaagatcgc gcctatgcgg agcggtatcg cgcgcggcta
     3781 gcgcgcgtcg ccgccgccga gcagcgcgcg aggcccggca gccaagcgct gcaggaagcc
     3841 gtggcgcgca actacttcgc cgtgctcgcc tataaggacg agtacgaggt cgcgcggctg
     3901 catacgcaga gcgggttcgt cgacagcgtc aagcgcaact tcggcaccgg cgtccgcatg
     3961 agctttcact tctctccgcc gctgttcgcg cgccgcgacg ccgcgacggg taggccgaag
     4021 aagtacgagc tcggcccttg ggtcctgccc atcttgcgtg tgcttgcaaa gctgcgttgg
     4081 ctgcgcggca gcaagctcga tccgttcggc cggagcgcgg atcggcgcct cgagcgcgag
     4141 ctcgtcgcgc gttacgaggc actgctcgat cgtatcgtcg ccgagctcga cgattcgcgt
     4201 tgcgagctcg cgctcgcgtt ggctcgtttg ccggagcaag tccgcggcta tggcccgatc
     4261 aaggccgccg ccgccgcgcg tgcgacaacg gcggagcagg agctgtggca gcaatgggcg
     4321 gcgccggcag cacgagcgga ccgcgcgcga gcgagcgcag ctcgagagcc tgcgcgcgcg
     4381 gcggcgcgct agtcagcgaa gccggcggcc cgattcagcg gctttggcgc agcgaatgca
     4441 gcggtcagcg aacggatacg cttcgagccg cgccatcgga atcgtctcgc cgcagtcggc
     4501 gcagatgccg taggtttggt tcttgatctg atcgagcgcc ttcgcgatct tgctgatctc
     4561 gctgcgggct tcgttgccga gcgcgtcgac gacctcggcg ttctcgagct cctgcgcctg
     4621 ctcttgcgaa tcggcgctgc gcccgcccgt caggttttct ttgaggcgct ccaagcgtcg
     4681 cgcgagctcg ccctgcttcg cgagcagctg ctctcggatc cgagcgtact ggtccatttt
     4741 tctcggccgt cccccgacgc cggttgcggg ctgtgcctcg cgcctactag catcgagcgc
     4801 acactggtcg aacgggttgg ttcgactcaa gcatagctcg tctcgcagcg tgaagcagag
     4861 gaaaactcga tgcgcttgct cgccggtacc agcggcttct catataaaga atgggtcggc
     4921 gagttctatc ccgaaaagac gcccgccaaa tcgatgctgg cctactacgc gacgaggctg
     4981 ccgatcgtcg agatcaacaa cacgttctat cggatgccca atcccgaggc gatccaaggc
     5041 tggcgcgccc aagtacctga ttcatttagc tttgccatca aggtgccgca gcgcatctcg
     5101 cacttcaagc ggctgcgcga ttgcggcgcg gacttacgcg tgttgctggc ggccctcgag
     5161 gacttgggcg ggagcttggg ctcgctgctc gtgcagctgc cgccgcatgc gcgctgtgac
     5221 accgccgcac tcgagtcgtt cttgagcgag ctgccgcccg gcgcgcgcgc agcgttcgag
     5281 tttcgtcacg actcatggat cgtcccgcgc gtgttcgagc tcttgcaagc gcgcaacgcc
     5341 gcactcgtgc agagcgagag cgacgacaaa ttcgagccgc tgccctggac cgccgactgg
     5401 gcgtatctgc gcctccgtaa agtcgactac acgcatgcgg atctcgtgcg ttggcacgcg
     5461 ctcatcgaag cggccggcgt cacggaggct caggtgttct tcaagcacga ggacggcgcc
     5521 accgggccga agctcgccag gcaattgctt gccttatcta actaaccggc cagcggcgaa
     5581 aacgctgccc aatcctccca ggcgttaaac aggtcgcact cggcggcctg tgagtgagag
     5641 cgaggccaca attgggc
//
LOCUS       DATLEE010000165         3230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9116554, whole genome shotgun sequence.
ACCESSION   DATLEE010000165 DATLEE010000000
VERSION     DATLEE010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9116554"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VM692_03540"
     CDS             <1..539
                     /locus_tag="VM692_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ51268.1"
                     /translation="KLEGVAVDVLINNAGIYSDRAACGDEACRGEDATQSFGKLDYEL
                     FDTIMAVNVKGPLLVSEAFIGHVQASRQKKLVSISSTNGSITFTLGGSGAIAYRASKA
                     ALNREMQLVAVNQRNAGVTVLLLHPGAVLTERQAHLTFPGMIEMQPSVAGMIDVIAKA
                     TIADTGRFIQYDGTDAPW"
     gene            561..1055
                     /locus_tag="VM692_03545"
     CDS             561..1055
                     /locus_tag="VM692_03545"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ51269.1"
                     /translation="MNTIKTFTLGGALLLAAGCGQTEKPTFAEDLAQITAFNERYLQS
                     INEEDIGTLSSLTTDGHVMLAPNSEPIVGRSANDAMNGSAFERYDFSETWMPVETVID
                     GNLAFQRGTFTTIATPKRDGERIEVKGSFMRIYQRQTNGEWRMTRDMFNSSTPLTRSL
                     STDG"
     gene            complement(1059..>3230)
                     /locus_tag="VM692_03550"
     CDS             complement(1059..>3230)
                     /locus_tag="VM692_03550"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HUQ51270.1"
                     /translation="CKEHYLSVARLREWHDVHDQLHELAQGLRWRTRQQTADYRALHQ
                     SVLAAFIDFIAEHDDGLVYRGMRESRAQLFANTPLAKKRPRWIVAAERVATERQYLRT
                     VAQVNPRWALRVAPHLVRFVYDDPQWDVERGQVTAREVVTLFGLTLASERRVDYGRVE
                     PKEARRLFIAEALAADRVGFKANEPAFLAHNRDILRTVHEAEARLRKRDLFVGEAGLA
                     AFYDDRLPQSVHDRASLASWSVGGRGAELAMSLLDAASRDPGDLEAAGYPRELTLAGQ
                     RLPLRYGFEPGGDSDGVTVLVPRALLGAIHAVELDWLVPAWLHEKLLAYLRALPKEQR
                     RPLVPLPATVAAVLAEIGDRAREQSVPIALAEGLRAARGVDVAPTAFDDRVLPAHLKL
                     RVAVVDVDGRVLAAGRDLRALQRELGAAPGALAAVAPVWPAEYRRSNLQRWDIGDLPE
                     SVIVAQRPRDLTLYPCLVDVDGRVELTLELPGPAAEALHRNGVRRLLLKALPQQTALI
                     RDRTLADRPLVLNYHGVGSSAALVDDVLCAAAEQAFELDPPVRTAGEFAARLDRGRSR
                     LVAEADALRALLAEILPLQRSLRRALDAAARSEAHAAVRDELVAQLDELVGPHMLTTT
                     PREWRPHLPRYLRAAELRWQKRGERDEPKRAEEVRVAAARLAHWRASRPAGVPWPAAV
                     VEYRWLLEELRVSLFAQQLKTIRPVSAKRLEQAWRKALARA"
BASE COUNT          547 a         1134 c         1100 g          449 t
ORIGIN      
        1 cgaagctcga aggcgtagcc gtagacgtgc tcatcaacaa cgcgggcatc tacagtgatc
       61 gcgccgcgtg cggcgacgag gcttgtcgcg gcgaagatgc cacgcagtct ttcggcaagc
      121 tcgattacga gctcttcgac acgatcatgg cggtcaacgt caagggcccg ctgctcgtct
      181 ccgaggcgtt catcggccat gtgcaagcga gccggcagaa gaagctcgtg tcgatcagct
      241 ccacgaacgg ctcgatcacg ttcacgctcg gcggctccgg cgcaatcgcc tatcgcgcga
      301 gcaaagcggc gctgaaccgc gagatgcagc tcgtcgccgt caatcaacgc aacgccggcg
      361 tcaccgtgct gctgttgcac cccggggccg tgctcacgga acggcaggcg cacctgacgt
      421 tccccggcat gatcgagatg cagccgagcg ttgccggcat gatcgacgtc atcgccaagg
      481 ccacgatcgc cgacacgggg cgattcattc aatacgacgg cacggacgcc ccctggtaag
      541 cgcgcccgcg gagactctcg atgaatacga ttaagacttt cacactcggc ggcgcgctgc
      601 tgctcgctgc gggctgcggc cagaccgaga aaccgacgtt tgccgaggac ctcgcgcaga
      661 tcacggcgtt caacgagcgt tacctgcaat cgatcaacga ggaagacatc ggcacgctga
      721 gcagcctgac gacggacggc cacgtcatgc tggcgccgaa cagcgagccg atcgtcggca
      781 ggagcgccaa cgacgcgatg aacggcagcg cgttcgagcg ctacgatttc agcgaaacgt
      841 ggatgcccgt agagaccgtc atcgacggca acctcgcgtt ccagcgcggc acgttcacga
      901 cgatcgcgac gccgaagcgc gacggcgagc ggatcgaggt caaaggcagc ttcatgcgca
      961 tctatcagcg ccagacgaat ggcgagtggc gcatgacgcg cgacatgttc aatagctcca
     1021 cgccgctgac gcgcagcctg tcgacggacg gttgagcctc aggcgcgggc gagcgccttg
     1081 cgccaggcct gctcgagccg cttcgcggaa acgggccgaa tcgtcttcag ctgctgcgca
     1141 aacagcgaga cgcgcagctc ctcgagcagc cagcgatact cgacgacggc cgccggccac
     1201 gggacgccgg ccggacgcga cgcgcgccaa tgcgcgagac gcgcagccgc gactcgcact
     1261 tcctcggcgc gtttgggctc gtcgcgctcc ccgcgcttct gccaacgaag ctccgcggcc
     1321 cgcaagtagc gcggcaaatg tgggcgccac tcgcgcggcg tcgtcgtcaa catgtgtgga
     1381 cctacgagct cgtcgagctg ggcgacgagc tcgtcgcgga ccgcggcgtg cgcttcgctg
     1441 cgcgcggccg cgtcgagcgc gcgccgcagg ctgcgctgca gcggtaggat ctcggccagc
     1501 agcgcgcgca gcgcgtcggc ttccgcgacg agccgcgaac gcccgcggtc gagccgtgca
     1561 gcgaactcgc ctgccgtgcg cacgggcggg tcgagctcga acgcttgctc cgcggcggca
     1621 catagcacgt cgtcgacgag cgctgccgag ctgccgacgc cgtgataatt caacacgagc
     1681 ggtcggtcgg ccaacgtgcg gtcgcgaatc agcgccgtct gctgcggcaa ggccttcaac
     1741 aatagccgcc ggacgccgtt gcggtgcagc gcctcggccg cgggtcccgg cagctccagc
     1801 gtgagctcga cgcggccgtc cacgtcgacg aggcacggat acaaagtcag gtcgcggggc
     1861 cgttgtgcga cgatgaccga ctccggcagg tcgccgatgt cccagcgctg cagattcgag
     1921 cgcctgtact ccgccggcca cacgggggca acggctgcaa gcgcgccggg cgccgcgccg
     1981 agctcgcgct gcaacgcgcg cagatcacgg ccggccgcga gcacgcggcc gtcgacgtcg
     2041 acgaccgcca cgcgcagctt caagtgcgcc ggcaacacgc ggtcgtcgaa cgccgtcggc
     2101 gcgacgtcga cgccgcgcgc ggcgcgcaag ccctcggcca gcgcgatcgg cacggactgc
     2161 tcgcgcgcgc ggtcgccgat ttcagccagc acggcagcca ctgtggccgg cagcggcacg
     2221 agcgggcgcc gctgctcctt cggcagcgct cgcagatacg ccaacagctt ctcgtgcaac
     2281 caggccggaa cgagccagtc gagctcgacg gcgtggatgg cgccgagcag cgcacgcggc
     2341 accagcacgg tgactccgtc ggagtcgccg cccggctcga agccgtaacg aagcggcagc
     2401 cgctggcccg caagcgtcag ctcgcgcggg tagccggcgg cctcgagatc gccgggatcg
     2461 cggctcgcgg cgtcgagcag cgacatcgcg agctcggctc cgcgcccgcc cacggaccag
     2521 ctcgcgaggc tcgcgcggtc gtgcacgctc tgcggcaacc ggtcgtcgta gaacgcggca
     2581 aggcccgctt cgccgacgaa caaatcgcgc ttgcgcagcc gcgcctcggc ctcgtgcacg
     2641 gtgcgcagga tgtcgcggtt gtgtgcgaga aacgccggct cgttcgcctt gaagcccacg
     2701 cggtcggcgg cgagcgcctc ggcgatgaac agtcgtcgcg cctcctttgg ctcaacgcgg
     2761 ccgtaatcga cgcgccgctc gctggccagc gtcaaaccga acagcgtcac gacctcgcgc
     2821 gccgtcacct ggccgcgttc cacatcccat tgcgggtcgt cgtagacgaa ccgcacgagg
     2881 tgcggcgcca cgcgcagcgc ccagcgcgga ttcacttgcg cgacggtgcg cagatattgg
     2941 cgttccgtcg cgacgcgttc ggcggcgacg atccagcgcg ggcgcttctt cgcgagcggc
     3001 gtgttggcga acagctgcgc gcgcgattcg cgcatgccgc ggtaaacgag gccgtcgtcg
     3061 tgctcggcga tgaaatcgat gaacgcagcg agcacggact ggtggagcgc gcggtagtcc
     3121 gccgtctgct gccgcgtgcg ccagcgtaag ccttgggcaa gctcgtgcag ctggtcgtgc
     3181 acgtcgtgcc attcgcgcag ccgcgccacc gataaatagt gctccttgca
//
LOCUS       DATLEE010000166         5293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7541908, whole genome shotgun sequence.
ACCESSION   DATLEE010000166 DATLEE010000000
VERSION     DATLEE010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7541908"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..603
                     /locus_tag="VM692_03555"
     CDS             103..603
                     /locus_tag="VM692_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51271.1"
                     /translation="MLTLLARLELTPFSVWIRESPSLLAFPFILYLHTLGLAMLAGIN
                     VGLDFWLLARRTVPLFNLTNIYRVMWLGFGINLLSGLALLAAYPAKALTNWVFFAKLT
                     AVVLGVYVLQQIKGELVVAEGASRDVTARARRLAIWSLLLWAGTIFAGRLLAYTYTIL
                     LASDGV"
     gene            603..1112
                     /locus_tag="VM692_03560"
     CDS             603..1112
                     /locus_tag="VM692_03560"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HUQ51272.1"
                     /translation="MENAFIDWVYSTRLSWLITTYGWLWPINESLHFCALTLLAGTVG
                     LFDLRLLGFAKGITPSSLHATLKWGIAGFIVSVVTGVQFFFGAPEQYYYNDAFRMKVV
                     FLVLMGANAALFYWREFPAIQTLGPHDDAPRSAKIVAAASLIFLVGVMLCGRMLTFFR
                     PPFFWNVNP"
     gene            1112..2182
                     /locus_tag="VM692_03565"
     CDS             1112..2182
                     /locus_tag="VM692_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HUQ51273.1"
                     /translation="MAVACDVVDVAGARKRWRNWAGVWAAVAAAACTPSPSPQIRTVT
                     GFAQGTTYSLQWLDGGGEAEIRAAAERELERIDALLSNYRSDSTLETFNAMRTTAAIE
                     LPAELVALFGVAKTVHAASDGCFDPTVRPLVRAWGFDGDTPTVPPPAVIDAALTTVGL
                     DKLELVDATHVRKARVDLEIDMASIGQGYTAGRLGDLLEQHGSTNYLAEIGGEVVTRG
                     AKPGGLPWRIGVEDPVSGGAPGPALRMPAESRTAAITSGSYRHYLEADGKRFGHIIDP
                     RTGWAVEHDLLSVTVVGADAAESAAWATALLCLGPTAALETAERENLAALLWTGDGSR
                     PATLEVSRALASWGGLLAPPAR"
     gene            complement(2179..3147)
                     /locus_tag="VM692_03570"
     CDS             complement(2179..3147)
                     /locus_tag="VM692_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HUQ51274.1"
                     /translation="MIAGFAPSGGRLRPVGESLADLAGLVWIDLLNPSAEEETMLEAQ
                     LGVDIPTREEMQRLEVSGRLYREEEAVVMTATLPARTDSDDLLMAPVAFVIVGGKLIT
                     VRYHEPRVFKTFPQRAGESNLGCANGETVFVALLEATVDRMTDVLERAEEEVDTISRA
                     IFRTDQTKSVSPDLMNTLQLIGRKGDLCSNIRVCVLSLQRLAGFWGQILADKSNSKEM
                     RGRVRTLEHYLESLGDHSSFVSQKVTFLLDATLGMINIEQSNIIKIFSIAAGVFLPPT
                     LIASIYGMNFAFMPELKWVYGYPFAAVLMLASALLPFWYFKRRGWL"
     gene            complement(3264..4784)
                     /locus_tag="VM692_03575"
     CDS             complement(3264..4784)
                     /locus_tag="VM692_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HUQ51275.1"
                     /translation="MISASKNQRRTVVGAVALLSMLATVTVFAQGRNNQQQPAQPAQP
                     TAAQQAAAAQQAAQAAAQKMLGELRPAVQRLRDQERALAREREQRFRGELQRVEKQAQ
                     DAVARRNAAEARSNALDRAWNDNEKKISETQALLTESQGNLGELFGVTRQVAGDAATV
                     LQGSVLSTQYGVPAQGEERAEFLRRLAAARALPSILELERLWYEIVREMTDAGKVVKF
                     RAAVAPLDDPMTPEDESKSPVEKDVVRVGPFTVSSNGQYLGYLPSQKSLFELRGELVG
                     DLQALGRELQSAPPNSGYHRAVVDPASGALLSLYVERPNWIRRIEEGEVVGYVIIFVG
                     ALGALLAVFQGIYLLMTRLAVSSQLRNLNDPRPNNPLGRVLLAFRGDGKRIESPELAE
                     LRLSEAVLREVPRLERFQSFLRLAVAAGPLLGLIGTVIGMIITFRAIVASGTSDPRLM
                     AEGIGQAMIATVLGLGIAIPLLFINAGLAALSRGVTQTLDEQSQSMLADNARKATA"
     gene            complement(4781..>5293)
                     /locus_tag="VM692_03580"
     CDS             complement(4781..>5293)
                     /locus_tag="VM692_03580"
                     /inference="COORDINATES: protein motif:HMM:NF023358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HUQ51276.1"
                     /translation="RSQEEQIAELEQEIAALDVTAVDIQPLLQRMFDDLVQFVADDVP
                     FFKEDRETRINVLRELMARVDASPSEKYRRLLEAYQIEMEYGRTMSAYKQALADGREA
                     EMVRLGRVTLLYRTVEGGETGYWDNQRKEWVADPGAAQEIQQALSIAKEERAPDLIIV
                     PVPAPQGGRS"
BASE COUNT          887 a         1712 c         1697 g          997 t
ORIGIN      
        1 ctcatcgaag tcgagttggc gcccggcacg gaagcctcgc cgtggccctt catccacatg
       61 cgtaagcgtc ccagcgagat cttgcgtgcc tgaggcggag acatgctgac cctactggcg
      121 cggctcgagc tgactccgtt cagcgtatgg atacgcgaat cgccgtcgct gttggcattt
      181 cctttcatcc tttatctgca cactctgggg ctcgcaatgc ttgccggcat caacgtcggc
      241 ctcgacttct ggttgctggc acgcaggacg gtgccgctct tcaatctcac caacatctat
      301 cgcgtcatgt ggctcgggtt cggcatcaac ctgctctcgg gcttggcgtt gctcgccgcc
      361 taccctgcca aagcgctgac gaactgggtg ttcttcgcca aattgacggc cgtcgttctc
      421 ggggtttacg tgctgcagca gatcaagggc gagctcgtgg tcgccgaggg cgcatcgcgc
      481 gacgtcaccg cacgcgctcg gcgcctcgca atttggtcgc tgctgctatg ggccggcacg
      541 atcttcgcgg ggcggctgct cgcttacacg tacaccattt tgctcgcgag cgacggcgtc
      601 tgatggaaaa cgcgttcatc gattgggttt atagcactcg gctctcgtgg ttgatcacga
      661 cctacggctg gctttggccg atcaacgaat cgttgcactt ttgcgccctg actctgcttg
      721 caggcaccgt gggcctattc gacctgcgct tgctcggctt tgcgaaaggc atcaccccga
      781 gctcgctgca cgcgacgctt aagtggggca tcgcgggatt catcgtatcg gtcgtcacgg
      841 gcgtccagtt cttcttcggc gcgccggagc agtactacta caacgacgcc ttcaggatga
      901 aggtcgtgtt cctcgtgctc atgggcgcca acgccgcctt gttctactgg cgcgaattcc
      961 cagcgatcca gacgctcggc ccacacgacg acgcaccgcg cagcgcgaag atcgtcgcgg
     1021 ccgcgtcgct gatctttctc gtcggcgtga tgctctgcgg caggatgctg acgttcttcc
     1081 ggccgccgtt cttttggaac gtcaacccgt gatggccgtg gcctgcgacg ttgtcgacgt
     1141 cgccggcgct cgaaagcgat ggcggaactg ggcgggggtc tgggctgcgg tcgccgccgc
     1201 ggcatgcacg ccgtcgccgt cgccgcaaat tcggacggtc acaggcttcg cccaaggcac
     1261 gacttattcg ttgcaatggc tcgatggcgg cggcgaggcc gagattcgcg ccgctgcaga
     1321 gcgagagctc gagcgcatcg atgcgctgct ctcgaactat cggtccgact cgacgctcga
     1381 gacgttcaat gccatgcgca cgaccgcggc gatcgagctc ccggcggagc tcgttgcgct
     1441 attcggcgtc gcgaagacgg tacacgccgc gagcgatggc tgcttcgacc ccacggttcg
     1501 gccgctcgta cgtgcgtggg gcttcgacgg cgatacgccc accgtgccgc cgcccgctgt
     1561 catcgatgcc gccctaacga cagtcggcct cgacaagctc gagctcgtcg acgcgacgca
     1621 cgtccgcaag gcgcgcgtgg atctcgagat cgacatggcc agcattggcc aggggtatac
     1681 agccggacgc cttggcgact tgctcgagca acacggcagc acgaattact tggccgagat
     1741 cggcggcgaa gtcgttacgc gcggcgcaaa acccgggggg ctgccgtggc gcatcggcgt
     1801 cgaggatccc gtgagcggcg gtgctccggg tcccgcgttg cgcatgccgg ccgagtcgcg
     1861 tacggcggcc attacgagcg gatcgtaccg gcattatctc gaggccgatg gcaagcgctt
     1921 cgggcacatc atcgatccgc gcacgggctg ggcggtcgaa catgacttac tgtccgtgac
     1981 ggtcgtcggc gccgacgctg ccgagtcggc agcctgggcg accgccttgc tttgcttggg
     2041 gccgacggcg gcgctcgaga cggccgagcg ggagaatctg gccgcgctgc tctggaccgg
     2101 cgacggcagc cggccggcca ctctggaagt gagccgcgcg cttgcgagtt ggggcggcct
     2161 gctcgcgccg cccgcgcgct agagccagcc gcgccgcttg aaataccaga acggcaacag
     2221 cgccgatgca agcatcaaca cggcggcgaa cgggtagccg tagacccact tgagctccgg
     2281 catgaaagcg aaattcatgc cgtagatcga ggcgatcagt gtcggcggaa ggaacacacc
     2341 ggcggcgatc gaaaagatct tgatgatgtt gctctgctcg atgttgatca tgccaagcgt
     2401 cgcgtcgagc agaaacgtga ccttttgcga aacgaaagac gaatgatcgc ctagcgactc
     2461 gaggtagtgc tcgagcgttc gcacgcgacc acgcatctct ttgctattgc tcttgtcggc
     2521 caggatctgt ccccagaaac ccgccaagcg ctgcaaggac aatacacaga cgcgaatatt
     2581 cgagcacagg tcacccttgc gcccgatgag ctgcagagtg ttcatgaggt cggggctgac
     2641 ggacttcgtt tggtcggtgc ggaagattgc gcgcgatatg gtgtcgacct cctcctccgc
     2701 acgctcgagc acatcggtca tgcgatcgac ggttgcttcg agcagcgcca cgaatacggt
     2761 ttcgccgttc gcgcagccca aattcgattc gccggcgcgt tgcgggaacg ttttgaagac
     2821 gcgcggctcg tggtaccgaa ccgtaatgag cttgccgcct acgatcacga atgcgacggg
     2881 ggccatgagc aagtcgtcgc tatccgttcg tgcaggcaac gttgccgtca tgacgacggc
     2941 ttcctcctcg cggtagagcc gtccggaaac ctccagacgt tgcatctcct cgcgagtcgg
     3001 gatgtcgacg ccgagttgtg cctcgagcat cgtctcctct tcggccgacg gattcagcag
     3061 atcgatccag acaaggcccg cgagatcggc cagcgattcg ccgacgggac ggagtctgcc
     3121 gcctgaggga gcgaaaccag cgatcatccg ggcaccaata cagcggacac ctgcgtgtag
     3181 aggactctag cacgcacggc gcggatgagc ggaaaggtgg gttgaggggg tggtggggtc
     3241 gtttcgcgac cccaccacta aagctaagca gtcgctttgc gcgcgttgtc cgcgagcatc
     3301 gactgactct gctcgtcgag cgtttgcgtg acaccgcgcg acagggcagc cagaccggcg
     3361 ttgatgaaca acagcggaat agcgatgccg aggccgagta ccgtggcgat catcgcttgg
     3421 ccgatgcctt ccgccatcag ccgcgggtcg ctcgtgcccg aggccacgat ggcgcggaac
     3481 gtgatgatca tgccgatgac ggtgccgatg aggccgagaa gcgggccggc ggcgaccgcg
     3541 agacgcaaga acgactggaa gcgttcgagg cgtgggactt cgcgcagcac ggcctccgaa
     3601 aggcgcaact ccgcgagctc ggggctctcg atgcgtttgc cgtcgccgcg gaaagccagc
     3661 agcacacgac cgagcggatt gttcgggcga gggtcgttca ggttcctgag ctgcgacgac
     3721 acggcgaggc gtgtcatgag cagatagatg ccttggaaga cggcgagcaa cgcacccaaa
     3781 gcgccgacga agatgatcac ataaccgacg acttcacctt cctcgatgcg gcgaatccag
     3841 ttcggtctct ccacgtacaa gctcaacaga gcgccgctcg cggggtctac gacggctcgg
     3901 tggtaaccgc tgttcggcgg ggcagactgc aactcgcggc cgagcgcttg caggtcgccg
     3961 acgagctcgc cgcgcagctc gaacagtgac ttctggctcg gcaggtagcc caggtactgc
     4021 ccgttgctgc tgaccgtgaa cgggccgacg cgcacgacgt ccttttccac aggagacttg
     4081 ctctcgtctt cgggtgtcat cgggtcgtcg agcggcgcga cggcggcgcg gaacttcacg
     4141 accttcccgg cgtccgtcat ctcgcgcacg atctcgtacc acaagcgctc gagctccaag
     4201 atcgagggca gcgctctcgc agcggccaag cgccgcagaa attcggcgcg ctcttcgccc
     4261 tgcgccggca ccccgtactg cgtgctcaac accgacccct gcagcaccgt ggcggcatcg
     4321 cccgccacct ggcgcgtgac gccgaacagc tcgccgaggt tgccttggct ctcggtcaac
     4381 aacgcctgag tctcggaaat ctttttctca ttatcgttcc acgcccggtc gagcgcgttg
     4441 ctgcgcgctt ccgcagcgtt ccgccgtgcg acggcgtctt gtgcttgctt ttccacgcgt
     4501 tgcagctcgc cgcggaagcg ctgttcgcgc tcgcgcgcga gcgcgcgctc ctggtcacgc
     4561 aggcgctgca cggccgggcg caattcgccg agcattttct gcgcggccgc ctgtgcggcc
     4621 tgttgggcgg ccgccgcctg ctgcgcggcc gtcggttgcg ccggttgcgc cggctgctgc
     4681 tgattgttac ggccctgcgc gaagacagtc acggtcgcca gcatcgacaa gagggctacc
     4741 gccccgacaa ccgttcgacg ctgatttttc gaagcgctga tcacgagcgg cctccttgcg
     4801 gcgccgggac gggcacgatg atgaggtcgg gtgccctctc ctccttggcg atgctcagag
     4861 cttgttgaat ctcctgcgca gcgcccggat cggccaccca ctctttacgc tggttgtccc
     4921 agtagccggt ctctccgcct tcgaccgtgc gatacagcag cgtgacgcgg ccaagtcgca
     4981 ccatctcggc ctcgcggccg tcggcgagcg cctgcttgta cgcgctcatc gtgcggccgt
     5041 attccatctc gatttgatag gcctcgagca gccggcggta tttctccgac ggcgatgcat
     5101 cgactctggc catgagctcg cgcaagacgt tgatgcgggt ctcgcgatct tccttgaaga
     5161 acggcacgtc gtcggccacg aactgaacga gatcgtcgaa cattcgctgc agcagcggct
     5221 gaatgtcgac ggccgtgaca tcgagcgccg cgatctcctg ctcgagctcc gcgatctgct
     5281 cctcttggga gcg
//
LOCUS       DATLEE010000167         3558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5398829, whole genome shotgun sequence.
ACCESSION   DATLEE010000167 DATLEE010000000
VERSION     DATLEE010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5398829"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="VM692_03585"
     CDS             <1..1216
                     /locus_tag="VM692_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HUQ51277.1"
                     /translation="VAVGWSGYVGSFLHDLGLDIPTALRGAPGTPITDFSSGAEIGVG
                     AVNLPAIIGIAAVTGLLVLGVSESAFVNNIVVAIKVTVVIAFIAIGAFYVNPDLWSPL
                     IPPAQPPPVDGDAGIWSEIGTALWAVMTAKDTGQYGVAGLITAAATIFFAYIGFEAVS
                     TAGAESRNPSRDMPIGIIGSLLICTVLYILTSAVLVGIVPYTELNDPAPIAKAVNQIG
                     LPWFAVLVKIGAIAGLSSVMLVLLYGQTRIFYTMARDGLIPPVFANVHPKFKTPWVNT
                     LIVGVLACGFAGFMGLDALANLTNVGTLLAFAIVCITVIYLRITRPDMPRSFRTPLFP
                     FTPLLGAVMCLFLLMSLMAHEATRRFFVFYLLGGIVLYFVYGMWNSKLRKGIVVHGHE
                     VMAETPHSKGLD"
     gene            1223..1873
                     /locus_tag="VM692_03590"
     CDS             1223..1873
                     /locus_tag="VM692_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HUQ51278.1"
                     /translation="MELLLTAFDYFLHVDRHLGEFATEYGVWIYALLFLIIFLETGVV
                     VTPFLPGDSLLFATGALAGAGVIDVVGILSLLTAAAILGDNTNYFIGRTVGPRVFTEH
                     SRWLKREHLLRTQRFYEVHGGKAVVLARFVPIVRTFAPFVAGIGRMHYPRFLAFDVGG
                     GVLWVWGFGLLGYFTGALPFVKENFGLVTIAVIFISLLPLVVEVVRARANRARTVS"
     gene            1884..3140
                     /locus_tag="VM692_03595"
     CDS             1884..3140
                     /locus_tag="VM692_03595"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HUQ51279.1"
                     /translation="MSLGAVTLGALALAVILSCTSRLNVGLLAIALAWLLGVYVGGLP
                     LAEVTSGFPVDLFLTLAGVTLLFAQAHVNGTLDIVAHNAMRLCRGRVGVVPIMYFGLG
                     AALASAGPGNIATTGLLAPMAMATALRMRISPFLMAIMVGNGCNAGSLSPIAPTGIIV
                     TRLMAGIGLQDVQLQTWLYNLLAHAIVGFAGYFVFGGFKLLTRSDRVEVEAEPAQPLD
                     AHQVLTLGIIGLLIVTAIACQIFRVAGFNIGLAAFTGAVLLSLLRAADDGDAIKKMPW
                     GTIMMVSGVTVLVALLQKTGGMEIFANLLSSIATQATVVPVLAVIIGIVSAYSSTSSV
                     VLPAFLPTVPGLIENLGGGDALAIASTMNVAGHLVDVSPLSTIGALCIASVPAGDMSR
                     KLFNQLLAWGMSMTVVGAVICFVMFG"
     gene            complement(3145..>3558)
                     /locus_tag="VM692_03600"
     CDS             complement(3145..>3558)
                     /locus_tag="VM692_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51280.1"
                     /translation="GEFSSLHAIAIDSRGRLFIGDRDNNRIQLYDQDGKYLDCWYQFS
                     RPSGIFIDADDNLFVTDSESRDGANDGKDLAHPGWQRGIRIGSARDGSVRYFIPDPAP
                     TGGSSTAEGVAAIKGGAVVYGAEVGPKSFVRYERR"
BASE COUNT          495 a         1154 c         1166 g          743 t
ORIGIN      
        1 ggtcgccgtc ggctggtccg ggtacgtcgg aagcttcctg cacgacctcg gcttggacat
       61 acccaccgcg ctgcgcggcg cgccggggac gccgatcacg gatttctctt cgggcgccga
      121 gatcggcgtc ggcgccgtca acctgcccgc gatcatcggc attgcggcgg tcacggggct
      181 gctcgttctc ggagtgtcgg agtccgcgtt cgtcaacaac atcgtcgtgg cgatcaaagt
      241 caccgtcgtc attgcgttca tcgcgatcgg cgcgttctac gtcaaccccg acctctggtc
      301 gccgttgata ccgccggcgc aaccgcctcc cgtagatggc gacgccggca tttggagcga
      361 gatcggcacg gcgctctggg ccgtcatgac tgccaaggac acggggcaat acggcgtcgc
      421 gggcctcatc acggcggccg cgacgatctt cttcgcttac atcggtttcg aggccgtatc
      481 gacggccggc gccgagtcgc gcaatccgag ccgcgacatg ccgatcggca tcatcggctc
      541 gctgctgatt tgcaccgtgc tctacatact gacgtcggcc gtgctcgtcg gcatcgtgcc
      601 gtatacggag ctcaacgacc ccgcaccgat cgcgaaggcc gtgaatcaga tcgggctgcc
      661 gtggttcgcg gtcctcgtga agatcggtgc gatcgcgggc ctgtcgtccg tgatgctcgt
      721 gctgctctac ggccagaccc gaatcttcta cacgatggcg cgcgatgggc tgatcccgcc
      781 cgtgttcgcg aacgtgcatc cgaagttcaa gacgccgtgg gtcaacacgc tgatcgtcgg
      841 cgtgctcgcc tgcggattcg ccggcttcat gggtctcgac gcgcttgcga atctcacgaa
      901 cgtcggcacg ttgctggcct tcgcgatcgt ctgcatcacc gtgatctact tgcgtatcac
      961 gcgccccgac atgccgcgct cgttcaggac gccgttgttt ccgttcacgc cgctgctcgg
     1021 cgccgtcatg tgcctgttcc tgttgatgtc gttgatggct cacgaggcga cgcggagatt
     1081 cttcgtcttc tatcttctcg gcggcatcgt gctgtacttc gtctacggca tgtggaactc
     1141 caagttgcgc aaaggcatcg tcgtgcacgg tcacgaagtc atggccgaaa cgcctcattc
     1201 gaaagggttg gactgatcgc agatggagct tttgctgacg gcgttcgatt acttcctgca
     1261 tgtcgaccgc cacctcgggg agtttgcgac cgaatacggc gtctggatct acgcgctgtt
     1321 gttcctgatc atttttctcg agacgggtgt cgtcgtgaca ccgtttctgc ccggcgactc
     1381 gctgctgttt gcgacgggcg cgctcgcggg cgccggcgtc atcgacgtcg tgggcatctt
     1441 gtcgctgctg acggctgcgg cgatcctcgg cgacaacacg aactacttca tcggccgcac
     1501 ggtggggccg cgtgtcttca ccgagcacag ccgctggctg aaacgcgagc acctgctgcg
     1561 cacgcagcgc ttctacgagg tgcacggcgg caaggccgtc gtgctcgcgc ggttcgtgcc
     1621 gatcgtgcgg acctttgctc cgttcgtcgc ggggatcggc cgaatgcact atccgcgctt
     1681 cctcgcgttc gacgtcggcg gcggcgtcct ctgggtctgg ggcttcgggt tgttgggcta
     1741 cttcacgggg gcgctgccgt tcgtcaagga gaacttcggc ctcgttacga tcgctgtgat
     1801 tttcatttcg ctgctgccgc tcgtcgtcga ggtcgtgcgc gcgcgggcga atcgcgcacg
     1861 caccgtttca tgagccgctc gcaatgagct tgggcgctgt cacgctcggc gcactggcgc
     1921 tcgcggtcat cctctcgtgc acgtcgcggc tcaacgtcgg gctgctcgcg atcgcgttgg
     1981 cctggctgct cggcgtctac gtcggcggcc tgccgctcgc cgaggtcacc tcggggtttc
     2041 ccgtcgacct attcctgacg ctggccggcg tgacgctgtt gttcgcacaa gcgcacgtca
     2101 acggcacgct ggacatcgtc gcgcacaacg cgatgcggct ttgccgtggg cgcgtcggcg
     2161 tcgtgccgat catgtatttc ggcctcggtg cggcgttggc ctcggcgggc cccggcaaca
     2221 tcgcgacgac gggcctgctc gcgccgatgg cgatggcgac ggcgctgcgc atgcgcatct
     2281 cgccgttctt gatggcgatc atggtcggca acggctgcaa cgcgggctcg ctgtcgccga
     2341 tcgcgccgac gggcatcatc gtgacgcgcc tcatggccgg catcggcttg caggacgtgc
     2401 agctgcaaac gtggctctac aacttgctcg cgcacgcgat cgtcgggttc gccggctatt
     2461 tcgttttcgg cggcttcaag ttgctgacgc ggtccgatcg cgtcgaggtc gaggccgagc
     2521 ccgcgcagcc gctcgacgcg catcaagtcc tgacgctcgg gatcatcggt ttgctgatcg
     2581 tcacggcaat cgcctgtcag atcttccgcg tcgcgggctt caacatcggc ttggctgcgt
     2641 tcacgggcgc cgtgctgttg tcgctgctgc gggcagccga cgatggcgac gcgatcaaga
     2701 aaatgccgtg gggcaccatc atgatggtca gcggcgtcac ggtgttggtc gcgctgctgc
     2761 agaagaccgg cggcatggaa atctttgcga acctgttgtc gagcatcgcg acgcaggcca
     2821 ccgtcgtgcc cgtgctcgcc gtgatcatcg gcatcgtgtc cgcctactcg agcacgtcga
     2881 gcgtcgtgct acccgcgttc ctgccgaccg tgcctgggct catcgagaac ttgggcggcg
     2941 gcgatgccct tgcgatcgcc tcgacgatga acgtcgcggg gcacctcgtc gacgtgtcgc
     3001 cgttgtcgac gatcggcgcg ctctgcattg cctcggtgcc ggccggcgac atgagccgca
     3061 agctgttcaa tcagctcctg gcctggggca tgagcatgac ggtcgtcggc gccgtcatct
     3121 gcttcgtgat gttcggctag ccgctcagcg gcgctcgtaa cggacgaacg acttggggcc
     3181 gacctcggcc ccgtagacga cggcaccgcc tttgatagcg gcgacaccct cggccgtgct
     3241 gctgccgccg gtcggggcgg ggtccggaat gaagtagcgc accgagccgt cgcgcgcgct
     3301 gccgatgcga atgccgcgct gccatcccgg atgtgcgaga tctttgccgt cgttggcgcc
     3361 gtcgcgcgac tccgagtccg tgacgaacaa attgtcgtcg gcgtcgatga agatgccgct
     3421 cggtcggctg aactggtacc agcaatcgag gtacttgccg tcttgatcgt agagctggat
     3481 gcggttgttg tcgcgatcgc cgatgaagag gcgcccgcgc gagtcgatcg cgatcgcatg
     3541 caggctcgag aactcgcc
//
LOCUS       DATLEE010000168         3094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_187787, whole genome shotgun sequence.
ACCESSION   DATLEE010000168 DATLEE010000000
VERSION     DATLEE010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187787"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VM692_03605"
     CDS             complement(<1..372)
                     /locus_tag="VM692_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HUQ51281.1"
                     /translation="MTTADRKAASVALIGVPTDVGAGRRGASMGPEALRVAGLDKALR
                     SLGRGVIDRGNVAGPRNPEQPRSGEYRHLPEVAAWCRGVHDAVYESLTAGEIPVVMGG
                     DHSVAIGSVAAIARYCAARDTP"
     gene            472..2118
                     /gene="ggt"
                     /locus_tag="VM692_03610"
     CDS             472..2118
                     /gene="ggt"
                     /locus_tag="VM692_03610"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HUQ51282.1"
                     /translation="MDRLAADRFATRSVVMARHGMVATSQPLATEIGVATLRRGGSAV
                     DAAIAANAALGLTEPTSCGIGGDLFAIVWDAATERLHGLNASGRSPLSLSLDHFAHAG
                     LRAIPERGPLSVSVPGAVDGWFELHARFGKLPLRELLAPSIEYAREGFPVTEVIGAAW
                     AAGARVLQDAPGFAAVFMPNGHPPAVGEPFANVALAQAYELIASDGRAAFYDGPIAAA
                     IEQTVRGNGGRLGREDLAAHRSQWVEPVSATYRGWRVFELPPNGQGIAVLQMLNILEG
                     FDLGSLEWGSAEHLHLLIEAKKLAFEDRARFYADPEFAAVPVERLVAKDYAAARRALI
                     DRQRAALAPTHGDAAAGAGDTVYLAAADAAGNMVSLIQSNYIGFGSRVTVTPFGFGLQ
                     NRGSQFALDAQHANAYAPGKRPFHTIIPAFAMRGADELLAFGVMGAAMQPQGQVQVLS
                     NIVDFGMGLQAAGDAPRVRHEGSTEPTGAPPVAGGGTVHLELGFAAAASDGLRARGHR
                     IEPAGSPSAFGGYQAIKRDVRQRVYRGASESRKDGHAAGL"
     gene            complement(2401..>3094)
                     /locus_tag="VM692_03615"
     CDS             complement(2401..>3094)
                     /locus_tag="VM692_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUQ51283.1"
                     /translation="VVRDALTSAFNSAGQRCSALRLLCLPEHTADEILRMLRGAMDEL
                     VVGDPQFLATDVGPVIDGDAQAGLTRYIAEHERAVVHRYRLGPAHEHGLFVAPTVIEL
                     PRAAALTREVFGPVLHVVRYRAERLDGLVDEINGLGYGLTLGVHTRIDATAKAIAQRA
                     RVGNVYVNRNMIGAVVGVQPFGGMGLSGTGPKAGGPHYVPRFTTERTVTTNTAAVGGN
                     ATLLSLDAGPGP"
BASE COUNT          444 a         1081 c         1104 g          465 t
ORIGIN      
        1 cggcgtgtcc cgcgccgcgc agtaacgcgc gatggcggcg acggagccga tcgcgacgct
       61 gtgatcgccg cccatgacga cgggaatctc gccggccgtg aggctctcgt agacggcgtc
      121 gtgcacgccg cggcaccagg ccgcgacctc gggcagatgt cgatactcgc ccgagcgcgg
      181 ttgctcggga ttgcgggggc cggcgacgtt gccgcgatcg atcacgccgc ggccgaggct
      241 gcgcagcgct ttgtcgagcc ccgccacacg caaggcttcg gggcccatgg acgcgccgcg
      301 cctcccggcg ccgacgtcgg tcgggacacc gatcaacgcc acggacgcgg ccttccgatc
      361 cgccgtcgtc atggccccga gtgtgccacg cagagcggtg ccggtgttac ggtttcggtt
      421 cagccgtaac agcggcacca accggatctt ggcgcgcact agaatgcgcg cgtggaccgc
      481 ctcgccgccg atcgtttcgc tactcgctcc gtcgtcatgg cgcggcacgg catggttgcg
      541 acgagccagc cgctcgcgac cgagatcggc gtcgccacgt tgcggcgcgg cggtagcgcc
      601 gtcgatgccg cgatcgcggc caacgctgcg ctcggcctca cggagcccac gagctgcggc
      661 atcggcggcg atctattcgc gatcgtttgg gacgccgcga cggagcgtct gcatgggttg
      721 aacgcgagcg gccgttcgcc gctgtcgctg tcgctcgacc atttcgcgca cgccggcttg
      781 cgagcgattc ccgagcgagg ccccttgtcc gtcagcgtgc cgggcgccgt cgacggctgg
      841 ttcgagctgc acgcgcgctt cggtaagctg ccgctgcgcg agttgctcgc gccgtcgatc
      901 gaatatgcgc gcgaagggtt tcccgtgacc gaggtcatcg gcgcggcgtg ggccgccggc
      961 gcgcgcgtgc tgcaggatgc gccgggcttc gccgccgtgt tcatgccgaa cggccacccg
     1021 cctgcggtcg gcgagccgtt cgcgaacgta gcgctcgcgc aggcgtatga gctcatcgcg
     1081 agcgacggac gcgccgcgtt ctacgacggc ccgatcgcgg cggccatcga gcagaccgtg
     1141 cgcggcaacg gcggccgcct cggccgcgaa gatctcgcgg cccatcgctc gcaatgggtc
     1201 gagccggtga gcgcgacgta ccgcggctgg cgcgtgttcg agctgccgcc gaacggccag
     1261 ggcatcgcgg tgctgcagat gttgaacatc ctcgaagggt tcgacttggg ctcgctcgag
     1321 tggggcagcg cggagcacct gcatctgctg atcgaagcga agaagctcgc gttcgaggac
     1381 cgtgcgcgct tctatgccga ccccgagttc gcggccgtgc ccgtggaacg gctcgtcgcc
     1441 aaggactatg cggccgcgcg ccgcgcgttg atcgatcgcc aacgcgccgc actggcgcct
     1501 acgcacggcg acgcggcggc cggcgccggc gacacggtct acctggccgc ggccgacgcg
     1561 gccggcaaca tggtctcgct gatccagagc aactacattg gcttcggctc ccgcgtgacc
     1621 gttacaccgt ttggcttcgg attgcaaaat cgcggctcgc aatttgcgct cgacgcgcag
     1681 cacgccaacg cttacgcgcc cggcaagcgc ccgtttcaca cgatcattcc ggccttcgcg
     1741 atgcgcggcg ccgacgaact gctcgcgttc ggcgtcatgg gcgctgcgat gcagccgcaa
     1801 gggcaagtcc aggtgctgtc gaacatcgtc gacttcggca tgggcctaca ggccgccggc
     1861 gatgcgccgc gcgtgcgcca cgaaggctcg acggagccga cgggcgcccc gccggtcgcg
     1921 ggcggcggca cggttcacct cgagctcggg ttcgcggccg ccgcgagcga cggcttgcgt
     1981 gcccgcgggc atcgcatcga gcccgccggc agcccgagcg cgttcggcgg ctatcaggcc
     2041 atcaagcgcg acgtgcggca gcgtgtctac cgcggcgctt ccgagtcgcg taaggacggt
     2101 cacgcggcgg gcttgtgacc gcgctgcaaa agaagagcag cgcccgaagg cgctgcaaag
     2161 ggggaattag tgggcagaag cggctgcggc gcagtgaaat cgatgggggg gatgcgaatt
     2221 caagtgacta ccgcgaccga ccctgcccgc ttagtactta gcaagcgccg tgccaagtcg
     2281 gctgcgagca aattcgcggg ttctgcttgg taggcgacgc gccgctgcac cgttttcgcg
     2341 cggcctgatc ggcgcgtgcc ccgatagcgc gcggcgcgtt tcaatgcctg ctgaaccgcc
     2401 tcaaggtccg ggacccgcgt cgagcgacag cagcgtggcg ttgccgccga cggcggccgt
     2461 gttcgtcgtg acggtccgct cggtcgtaaa ccgcggcacg tagtgcgggc cgccggcctt
     2521 cggcccggtt cccgacaacc ccatgccgcc gaacggctgc acgccgacga cggcgccgat
     2581 catgttgcgg ttgacataga cgttgccgac gcgcgcacgc tgcgcgatcg ccttcgccgt
     2641 cgcatcgatg cgtgtatgca cgccgagcgt gaggccgtag cccaagccgt tgatctcgtc
     2701 gacgaggccg tcgagccgct cggcccggta gcgcacgacg tgcagcacgg ggccgaacac
     2761 ttcgcgcgtc agcgccgcgg cgcgcggcag ctcgatgacg gtcggcgcga cgaacaagcc
     2821 gtgctcgtgc gccggcccga gccggtagcg gtgcacgacg gcccgctcgt gctcggcgat
     2881 gtagcgcgtc agccccgcct gcgcgtcgcc gtcgatgacg ggcccgacgt cggtcgcgag
     2941 gaactgcgga tcgccgacga cgagctcgtc catggcgccg cgcagcatgc gcaagatctc
     3001 gtcggccgtg tgctcgggta ggcacaacag gcgcagcgcc gagcagcgct ggcccgcgct
     3061 attgaacgcc gaggtcaacg cgtcgcggac gact
//
LOCUS       DATLEE010000169         2786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_188299, whole genome shotgun sequence.
ACCESSION   DATLEE010000169 DATLEE010000000
VERSION     DATLEE010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188299"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..493)
                     /gene="ribE"
                     /locus_tag="VM692_03620"
     CDS             complement(17..493)
                     /gene="ribE"
                     /locus_tag="VM692_03620"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HUQ51284.1"
                     /translation="MDERIKAFEGDFNARGLRFGIVASRFNDFIVDRLLDAAVGTLLK
                     HGVAPGDIEVVRVPGAFETPLAIKKLATSRRFQALIALGCVIRGGTPHFEYVAGEATR
                     GIGQVGLSEQIPIGFGLLTVETIEQAIERAGTKAGNKGADAALAAIQMATVLRQLE"
     gene            complement(486..1601)
                     /gene="ribBA"
                     /locus_tag="VM692_03625"
     CDS             complement(486..1601)
                     /gene="ribBA"
                     /locus_tag="VM692_03625"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HUQ51285.1"
                     /translation="MNSTEDIIAAIRAGEMVVILDDEERENEGDLVMAATKVRAEDIN
                     FMAKYGRGLICLTLTRARCEQLRLPLMVSNTDIRQTTNFTISIEAAEGVTTGISAHDR
                     ARTVQAAVAPAAQPRDLRQPGHIFPLMAQPGGVLTRAGHTEAGCDLARLAGLEPAAVI
                     VEILNEDGAMARRDDLVKFARRHTLKIGTIADLIEYRLRTEESVEIIGESEVETEFGA
                     FRLVCFEDHVNRAVHLALVRGQIDAKKPTLVRVHRQDTLSDVLGLHAPALHRPLRDAL
                     QLIAAEGEGVAVILRPEESARELVRSVQSLMQPPEPEVGLRRHRNMDFRTFGIGAQIL
                     RALGVRRMRVVGAPKHMHGLSGFGLEVVEYVSDESHG"
     gene            complement(1615..2268)
                     /locus_tag="VM692_03630"
     CDS             complement(1615..2268)
                     /locus_tag="VM692_03630"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HUQ51286.1"
                     /translation="MFSGLVKGVGRIATRTAHGDDRRFVVELGSAAAAPLAVGGSIAV
                     NGVCLTAIEVTPKSFAADVSAATLAVTTLGALAAGDAVNLEPPLRAGDPLDGHIVTGH
                     VDGVGEVVTIEPAGRSTRLVIEVPAPLSRYIAAKGSVAVDGVSLTVNAVDGARFEVNI
                     IPHTQSVTVIGGYARGTAVNIEVDMLARYLERLVRAEPDGGIDLAFLRKHGYARDHR"
     gene            complement(2331..>2786)
                     /locus_tag="VM692_03635"
     CDS             complement(2331..>2786)
                     /locus_tag="VM692_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HUQ51287.1"
                     /translation="PLRVVVDGRLKMPASAKTLTVPGEVVVLTSRSVDDPKAVALRAA
                     GARVERVAGDEEHCDLRAVVEHLGKLEVNDVWIEAGAGFNGAMLRAGLIDELIIYMAP
                     RLLGDSARGMFSVPALHTLAESWKVDYDDFVLVGSDLRITARVSRVAGA"
BASE COUNT          456 a          956 c          900 g          474 t
ORIGIN      
        1 tcacgcgtca aaccgactac tccagctgcc gcagcacggt agccatctgt atggctgcga
       61 gtgctgcgtc cgcgcctttg ttgccggcct tcgttccggc gcgctcgatg gcctgctcga
      121 tcgtctcgac ggtcaaaagt ccgaacccga tcgggatctg ctcgctcaag ccgacttggc
      181 cgatgccgcg tgtggcttcg ccggccacat attcgaagtg cggtgtcccg ccgcgaatca
      241 cgcagccgag cgcaatcagc gcctggaaac gccgactcgt tgcgagcttc ttgatggcga
      301 gcggcgtctc gaatgcgccc ggtacgcgca cgacctcgat gtcgcccggc gccacaccgt
      361 gcttcagcaa ggtgccgacg gcggcatcga gcagacggtc gacgatgaag tcgttgaaac
      421 gcgacgcgac gatgccgaag cgcaaaccgc gcgcattgaa gtcgccttcg aacgctttga
      481 tccgctcatc catgcgattc atccgagacg tattcgacaa cctccagccc gaaccccgag
      541 aggccgtgca tgtgcttcgg cgcgccgacg acgcgcatgc gccgcacacc gagcgcgcgc
      601 aggatctgcg cgccgatgcc gaacgtccgg aaatccatgt tgcggtggcg acgcaagccg
      661 acctcgggct ccggcggctg catcagggac tgcaccgagc gcacgagctc gcgcgccgac
      721 tcctcgggcc gcaggattac cgccacgccc tcgccctctg cggcgatcag ttgcagcgcg
      781 tcgcgcaggg gccgatgcaa ggctggcgcg tgcagaccga gcacgtcgct cagggtgtcc
      841 tgccgatgca cgcgcacgag cgtcggcttc ttggcgtcga tctggccgcg caccagggcg
      901 aggtgaacgg cgcgattgac atgatcttcg aagcagacga ggcgaaacgc gccgaactcc
      961 gtctcgacct cgctttcgcc gatgatctcg accgactcct cggtgcgcag gcgatattcg
     1021 atgagatcgg cgatcgtgcc gatcttgagc gtatggcgcc gcgcgaactt gacgaggtcg
     1081 tcgcggcgcg ccatcgcgcc gtcttcgttc aagatctcga cgatgacggc ggccggctcg
     1141 aggccggcga gccgggcgag gtcgcagccg gcctccgtgt gtcccgcccg cgtcagcacg
     1201 cccccgggtt gcgccatcag cggaaagatg tggcccggct gacgcaggtc gcgcggctga
     1261 gctgccggcg caaccgcggc ttgcaccgtg cgcgcgcgat cgtgcgccga gatgcctgtc
     1321 gttacgcctt cggccgcctc gatcgaaatc gtgaaattcg tcgtttgccg aatgtcggta
     1381 ttgctgacca tcaacggcag gcgcagctgc tcgcagcgcg cgcgcgtcag cgtcaggcag
     1441 attagcccgc ggccgtattt cgccatgaag ttgatgtctt cggcgcgcac cttggtggcc
     1501 gccatgacga ggtcgccttc gttctcgcgc tcctcgtcgt cgagaatcac gaccatttcg
     1561 ccggcgcgaa ttgccgcgat gatgtcctcg gtcgagttca tctggacgaa agggctatcg
     1621 gtggtcgcgt gcgtacccat gtttcctcag gaaggcgaga tcgattccgc cgtcgggctc
     1681 cgctcgcacg aggcgctcga gatagcgagc cagcatgtcg acctcaatat taacagcggt
     1741 gccgcgcgca tacccgccga tcacggtcac ggactgcgta tgcggaatga tgttgacctc
     1801 gaacctcgcg ccgtcgacgg cgttgaccgt gaggctcaca ccgtcgacgg ctacggagcc
     1861 tttcgcagcg atgtagcgcg acagcggggc cggcacttcg atgacgagcc gcgtcgacct
     1921 gccggccggc tcgatcgtga cgacttcgcc gacgccatcg acgtgacccg tgacgatgtg
     1981 gccgtcgagc gggtcaccgg ctctgagcgg cggctcgaga ttcacggcgt cgccggcagc
     2041 gagcgcgccg agtgtcgtga ccgcgagcgt cgcggccgat acgtccgcgg caaagctctt
     2101 cggcgtgact tcgatggccg tcaggcatac gccgttgacg gcgatgctgc cgccgaccgc
     2161 gagcggcgcg gcggcagcgc tgccgagctc gacgacgaag cggcgatcgt cgccgtgagc
     2221 cgtgcgcgtc gcaattcgtc cgacgccttt gaccagtccc gaaaacacgc gcttctctcc
     2281 gctcggcaat tgctacatgc attgctgtac cgcgcgcatc cctgcccgcg tcaggcgccc
     2341 gccaccctac tgacgcgagc cgtgatccgc aagtcgctgc cgactaggac gaagtcgtcg
     2401 tagtcgacct tccaactctc ggccaacgta tgcagcgccg ggacgctgaa catgccgcgc
     2461 gccgagtcgc ccagcaaccg cggcgccata tagataatga gttcgtcgat gagcccggct
     2521 cgcagcatcg cgccgttgaa gccagcgccg gcctcgatcc aaacgtcgtt gacctcgagc
     2581 ttgccgaggt gctcgacgac ggcgcgcaga tcgcagtgct cctcgtcgcc cgcgacgcgc
     2641 tcgacacgtg cgccggccgc gcgcagtgcg acggctttcg gatcgtcgac cgagcgtgac
     2701 gtcaacacca cgacttcgcc cggcacggtc aaggtcttgg ccgacgcggg catcttgagt
     2761 ctgccgtcga cgacgacgcg cagcgg
//
LOCUS       DATLEE010000170         6381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6056140, whole genome shotgun sequence.
ACCESSION   DATLEE010000170 DATLEE010000000
VERSION     DATLEE010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6056140"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1282)
                     /locus_tag="VM692_03640"
     CDS             complement(140..1282)
                     /locus_tag="VM692_03640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUQ51288.1"
                     /translation="MGHRLRALLALTALFAAHAAQATEVAVCTDRGRAVLELADEAAP
                     LQVANFLRYVDMGYYSGTVFHRVVPEFVVQGGGLDRQLRARSTLPPVANEARNGLRNA
                     RGTVAAARTQDPDSASSQFFVNLVDNAALDAGGREAGYTVFARVTEGLDVFEAIARLP
                     TGAAGPFQSDVPRPLVTIKSIARLDAAALAALPAPGREAGLRGEIAASAEAANAAVAL
                     RLIDQYRAICGADDTEIALAEARMALAANERQRAVFALDELLATTDTAHPAHEAAMAL
                     YAQATAEPSATPQTVGGCAPPAAPQLPDAAVATEDQMVASQRQVRAFVAAGETHLACL
                     AKIIDDKDRAADERNAAVGEHNRMVAAMEEIAAAFNERIRVFKTRG"
     gene            1494..1883
                     /locus_tag="VM692_03645"
     CDS             1494..1883
                     /locus_tag="VM692_03645"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51289.1"
                     /translation="MARSSLPVLSLLGAFALVTDVAAHHSVAGQFDAAKRTEITGTVS
                     KVEWINPHVYIYMDVADDKGAVTTWRLESLPTAMLRKAGLTSEMIKGGGQKVTAAALM
                     ARNGTPNLAWLLNLRYEDGHEFTLAGE"
     gene            1895..2698
                     /locus_tag="VM692_03650"
     CDS             1895..2698
                     /locus_tag="VM692_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51290.1"
                     /translation="MRNTETRASKVLVGVCLAFAAPLAAQQGAVPRTADGHPDLSGTW
                     DNGAGIDFVQPTKDGKSVCIFGCTGGGAPPPADGPAPRQTTPQYRPEFAARVADLDKR
                     QVQEDPVLRCFAPGVPRIGPPDKIVQRPGEAVFLYEDVSGSFFRIVPTDGRPHPTNNE
                     PSFLGDAVGHWEGDTLVIETKNFNDQTWLTDNGAFHTTDLRVVERLSRTANTLEWTAT
                     AHDPAVLTQPWELAKRVAQPTDIEIVESPPCIDRSLANMVDDSHHTNPR"
     gene            complement(2876..3292)
                     /locus_tag="VM692_03655"
     CDS             complement(2876..3292)
                     /locus_tag="VM692_03655"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ51291.1"
                     /translation="MDLEASVSVHFNGDCREALEFYERLLGAKLELVLTWGQTPMAAD
                     APPSWHDKILFARLTSKNMSLVGADTLPGTYRAPTGFNLSLATTDLAETERYFAELAR
                     GGVVHQPLGETFWALRYGFVTDRFGVPWEITCAKPQ"
     gene            complement(3300..4562)
                     /locus_tag="VM692_03660"
     CDS             complement(3300..4562)
                     /locus_tag="VM692_03660"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUQ51292.1"
                     /translation="MKLATAAVGAALTFVSFARVEAATNPEAVGLARERLERIDAMIE
                     RRIAAGDLTGAVAIVARRGQVAHVAIHGTMDSDTRLPMRADTLFRIASMTKPIVGVGI
                     MMMVEENKLRLTDPVARYIPEFANLRVAVARPEGGSAAAPAFDLAAPQRQVTIKDLLS
                     HVSGLGSGPMSNSTIDAVARREGETLADYIPRLGGTALEFQPGTRWAYSPGAGFETLG
                     RVLEIASGMPLDEFFATRIFAPLGMRDITFWPSDSQWPRVATVYRREESGLVKQTMPN
                     DSFSRNVYFRGSGGLYSTAESYVPFGMMLANGGELNGKRLLSRKSVEMLGAVHVPDTL
                     PGRPAGEGFGLSVRVVTDHAARGTLLSDGTYGWSGAQGTHFFVDPKEELVGVLMIQTP
                     NITEIRADFENLVAQAIVDDPWKSPKGD"
     gene            complement(4633..5454)
                     /locus_tag="VM692_03665"
     CDS             complement(4633..5454)
                     /locus_tag="VM692_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HUQ51293.1"
                     /translation="MNKSTILTALSAGILAALATGARAQDDERLVVPLSDPSRPAVLE
                     VALFSGDIQVESYDGNEIIIVADAPMKGPEGTEEPRADGLRRIQSSSVGLTVEEGDNK
                     VSLRMDFSPKNVDLEVRVPRRTSVHANLVNGGDIEIMGVTGEHELQNVNGDVIATDIS
                     GSAVLNSTNGDVRATFASVAGESPMSFTSFNGDVDVSLPANLSADLLVTSQQGDVFTD
                     FDFQERQNPTDVRRQQDAGGRRVVRMQRETRYAIGGGGRDIQLRTFNGDIMIRKR"
     gene            complement(5451..6311)
                     /locus_tag="VM692_03670"
     CDS             complement(5451..6311)
                     /locus_tag="VM692_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HUQ51294.1"
                     /translation="MNVSLCIIAAAASAALLASAGDASADVVDTRELDETVPVTAGAP
                     LVVIVKNITGSVRVTQHDRDHVEMHATETVRGDLQSDIERARAELALRTESEPGRVAF
                     RVRRTGDDDGNRRDWNWGDDYTVEYDIEVRVPRGATVEASTVNDGDVTVEGVTGDFKL
                     VNVNGEIHATGLTGSGEIRTVNGNVAAAFARAPQQATSFHTVNGDLDVTFPNDLSADL
                     NFKTMNGDVFTDFDVESLGGTAAVDRTRNGGRSMMRMNRNTAFRVRSGGETHSFNTLN
                     GEIYVRKAAR"
BASE COUNT         1000 a         2137 c         2169 g         1075 t
ORIGIN      
        1 gatgtagtgg ccgacgacgc tgggcggcgg ggcattcggc gccagcgcct cgacgagcca
       61 tggcgcgctc ggctcgagat gcttgcgagg atcgttggcg ctcatgccgc gatcttaccg
      121 cggactggcg gcttcagctt cagccgcgcg tcttgaagac gcggatgcgc tcgttgaagg
      181 cggccgcgat ctcttccatc gctgcgacca tgcggttgtg ctcgccaacg gccgcgttgc
      241 gctcgtcggc cgcgcggtcc ttgtcgtcga tgatcttcgc gaggcaggcg aggtgcgtct
      301 cgccggccgc cacgaacgcg cgcacctgcc gctgcgaggc gaccatctgg tcttcggtcg
      361 cgacggcggc gtccggcaac tgcggcgcgg ccggcggcgc gcagccgccg acggtttgcg
      421 gtgtcgccga gggctcggcc gtcgcttgcg cgtataacgc catcgcggcc tcatgcgccg
      481 ggtgcgccgt gtccgtcgtc gcgagcaact cgtcgagcgc gaacaccgcg cgctggcgtt
      541 cattggctgc gagcgccatg cgcgcttcgg ccaaggcaat ttcggtgtcg tcggcgccgc
      601 agatggcgcg gtactgatcg atgaggcgta gcgccacggc ggcgttcgct gcctcggccg
      661 aggctgcgat ctcgccgcgc agcccagcct cgcgtcccgg cgcgggcaga gccgccagtg
      721 ccgccgcgtc gagccgcgcg atcgacttga tcgtgacgag cggcctcggc acgtcgcttt
      781 ggaacgggcc ggcggctcct gtcggcaggc gcgcgatcgc ttcgaagacg tcgaggcctt
      841 ccgtgacgcg cgcgaacacc gtgtaaccgg cctcgcggcc gccagcgtcg agcgccgcgt
      901 tgtccacgag attgacgaag aactgcgagc tcgccgaatc gggatcttgc gtccgcgccg
      961 ccgctacggt gccgcgcgcg ttgcgcaagc cgttgcgagc ttcgttggca acgggcggca
     1021 acgtcgaccg cgcccgcagc tggcgatcca agcctccgcc ctgaacgacg aactccggca
     1081 ccacgcgatg gaacaccgtg cccgagtagt agcccatgtc gacgtagcgc aagaaattcg
     1141 cgacttgcag cggcgcggcc tcgtcggcca gctcgagaac ggcgcggcca cgatccgtgc
     1201 agacggcgac ctccgtcgcc tgcgcggcat gcgcggcgaa cagggcagtg agcgccagca
     1261 gcgcgcgcaa gcgatgaccc atgaggcgcg atcccccgat cgagtggggg gccgagcata
     1321 gcactccgtc ctgacggagc acgccgtcgt cggccggcga caaggagcgg cgcgtgcgtg
     1381 tcacgagacc acggtgggac cggctcgcga cggggcctca cgctagcgat tctgtgcgcg
     1441 cactgcgcta agatcgtacc cacactcaag aacgcacggg cgggggattc gccatggcac
     1501 gcagctcttt gccggtactc tcgctgttgg gcgcgttcgc gctcgttacc gatgtcgccg
     1561 cgcatcactc cgtcgctggt cagttcgacg cggcgaagcg cacggagatc acgggcacgg
     1621 tcagcaaggt cgagtggatc aatccgcacg tctacatcta catggacgtc gccgacgaca
     1681 aaggcgcggt cacgacttgg cgtctcgagt cgctgccgac agcgatgctg cgcaaggccg
     1741 ggctcacgag cgagatgatc aagggcggcg gccagaaggt gacggccgcg gcgctgatgg
     1801 cgcgcaacgg cacgccgaac ttggcgtggc tgctgaacct caggtacgaa gatggccacg
     1861 aattcacact agccggcgaa tgacggagcg aacgatgcgt aacaccgaaa cacgtgcttc
     1921 gaaggttctc gttggcgtat gcctcgcttt cgccgcgccg ctcgcggcgc agcaaggagc
     1981 cgtgccgcgc acggcggacg gtcatcccga tttgagcggc acctgggaca acggcgctgg
     2041 catcgacttc gtgcaaccga cgaaagacgg caagtcggtc tgcatcttcg gctgcaccgg
     2101 cggcggtgcg ccgccgcccg cggacgggcc ggccccgcgt cagaccacgc cgcagtatcg
     2161 ccccgagttc gcggctcgcg tcgccgatct cgacaagcgg caagtgcaag aggacccggt
     2221 gttgcgctgc tttgcgcccg gcgtgccgcg catcgggccg cccgacaaga tcgtgcagcg
     2281 gcccggcgaa gccgtcttcc tttacgagga tgtctcgggc agcttcttcc gcatcgtgcc
     2341 gactgacggc cggccgcacc cgacgaacaa cgagccgagc tttttgggcg acgccgtcgg
     2401 tcattgggag ggcgacacgc tcgtcatcga gaccaagaac ttcaacgatc agacgtggct
     2461 gaccgacaac ggcgcattcc acacaacaga cctgcgcgtc gtcgagcgtt tgagcaggac
     2521 ggcgaatacg ctcgaatgga cggcgacggc gcacgatccc gccgtgctca cgcagccttg
     2581 ggagctcgca aagcgcgtcg cgcaaccgac cgacatcgag atcgtcgagt cgccgccgtg
     2641 catcgaccgc tcgctggcga acatggtcga cgattcacac cacacgaatc cgcgctgagc
     2701 gctacacgaa gatctgccgg tcgaccggcc gctgcggcgg cgcgggctcg gtcgttcctc
     2761 ccgctaccgc ccgggccgct cgggcggtag cggggacaat accctcgctc gtactcgcgc
     2821 ccgggcagga gcgcgttgcg acgatccgat cttcgctccg gcgggccctc caacgtcact
     2881 gcggcttcgc gcaggtgatt tcccacggca cgccgaagcg gtcggtgacg aagccgtagc
     2941 gcaatgccca gaacgtttcg ccgagcggtt gatgcacgac gccgccgcgc gcgagctcgg
     3001 cgaaatagcg ttccgtttcg gccaggtcgg tcgttgctag cgacaggttg aagcccgtcg
     3061 gcgcgcgata agtacccggc agagtatcgg cgccgacgag cgacatgttc ttggacgtca
     3121 ggcgcgcgaa caggatcttg tcgtgccagc tcggcggcgc gtcggccgcc atcggcgttt
     3181 ggccccacgt cagcacgagc tcgagcttgg cgccgagcag gcgttcgtag aactcgagcg
     3241 cctctcggca gtcgccgttg aagtgcacgc tgacgctcgc ctcgaggtcc atggctgctt
     3301 cagtccccct tcggggactt ccaagggtcg tcgacgatcg cttgggcgac gaggttctcg
     3361 aaatcggcgc ggatctccgt gatgttcggc gtctggatca tcagcacgcc gacgagctct
     3421 tctttcgggt cgacgaagaa gtgcgtgccc tgtgcgccgg accagccgta cgtgccgtcc
     3481 gacaacagcg tgccgcgcgc cgcatggtcg gtcacgacgc gcacgctcaa gccaaagccc
     3541 tcgccggcag ggcggcccgg caacgtgtcg gggacgtgca ccgcgccgag catctcgact
     3601 gacttgcgcg acagcagccg cttgccgttg agctcgccgc cgttcgccaa catcatgccg
     3661 aacggtacgt agctttccgc cgtgctgtag aggccgcccg agccgcggaa gtaaacgttg
     3721 cgcgagaagc tgtcgttcgg catcgtctgc ttgacgaggc ccgactcctc gcggcgatag
     3781 accgttgcaa cgcgcggcca ctgcgagtcg ctcggccaga acgtgatgtc gcgcatgccg
     3841 agcggcgcaa agatgcgcgt cgcgaagaac tcgtcgagcg gcatgccgga ggcgatttcg
     3901 agcacgcggc ccagcgtctc gaagccggcg ccggggctgt aggcccagcg cgtgccgggc
     3961 tggaactcga gcgccgtgcc gccgagccgc ggaatgtaat ccgcgagcgt ctcgccttct
     4021 cggcgcgcta cagcgtcgat cgtgctgttg ctcatcgggc cgctaccgag gcccgagacg
     4081 tgactgagca gatccttgat agtgacctga cgttgcggtg cggcgaggtc gaaggccggc
     4141 gcggcggcgc tgccgccttc gggccgcgcc actgcaacgc gcaggttcgc gaactccgga
     4201 atgtagcgcg caacggggtc cgtcaagcgc agcttgttct cctcgaccat catcatgatg
     4261 ccgacgccga cgatcggttt cgtcatcgag gcgatgcgga acagcgtatc ggcgcgcatc
     4321 ggtagccgcg tgtcgctgtc catggtgccg tggatcgcga cgtgcgcgac ctggcctctg
     4381 cgcgcgacga tcgccacggc gcccgtgaga tcgccggccg caatgcgccg ctcgatcatg
     4441 gcgtcgatgc gctcgaggcg ctcgcgcgct aagccgacgg cttcgggatt cgtcgcggct
     4501 tcgacgcggg cgaacgatac gaacgtgagc gccgctccga ctgcggccgt tgctagcttc
     4561 atggcacccc ccctcgaacc tcgaagggga tggtcgaacg gcggcccgcg ttttgcaacc
     4621 cggcggccgc gcctaccgct tgcggatcat gatgtcgccg ttgaaggttc gcagctgaat
     4681 gtcgcggccg ccgccgccga tcgcgtagcg cgtctcgcgc tgcatgcgca cgacacgacg
     4741 cccgccggca tcctgctggc gccgcacgtc ggtcgggttc tgacgctcct ggaagtcgaa
     4801 gtccgtgaac acgtcgccct gctggctcgt gacgagcagg tcggccgaga ggtttgccgg
     4861 cagcgagacg tcgacgtcgc cattgaacga cgtgaacgac atcgggcttt cgcctgcaac
     4921 ggacgcgaac gtcgcgcgca cgtcgccgtt cgtcgagttc agcacggcag agcccgagat
     4981 gtccgtcgcg atgacgtcgc cgttgacgtt ctgcagctcg tgctcgccgg tcacgcccat
     5041 gatctcgatg tcgccgccgt tgacgagatt ggcgtgcacc gaggtgcggc gcggcacgcg
     5101 cacttcgaga tcgacgttct tcgggctgaa atccatgcgc agcgacacct tgttgtcgcc
     5161 ttcctcgacc gtcagcccca ccgacgagct ctggatgcgg cgtaagccgt cggcgcgcgg
     5221 ctcctcggtc ccttcggggc ctttcatcgg tgcgtcggcg acgatgatga tctcgttgcc
     5281 gtcatagctc tcgacctgga tgtcgccgga gaacaatgcg acctcgagca ccgccgggcg
     5341 ggatggatcg ctgagcggca cgacgagacg ctcgtcgtct tgcgcccgtg cgccggttgc
     5401 tagcgcggcg aggatgccag ccgagagggc ggtcagaatc gtggacttgt tcatcgggct
     5461 gccttgcgaa cgtagatttc gccattcagt gtgttgaagg agtgcgtctc gccgccggag
     5521 cggacgcgaa acgcagtgtt gcggttcatg cgcatcatcg atcgcccgcc gttgcgcgtg
     5581 cggtcgacgg ccgcggttcc ccccagggat tcgacgtcga agtccgtgaa gacgtcgccg
     5641 ttcatcgtct tgaaattcag atcggccgag agatcgttgg ggaacgtcac gtcgagatcg
     5701 ccgttgaccg tatgaaacga ggtcgcttgc tgcggcgcgc gggcgaacgc tgcggcaaca
     5761 ttgccgttga cggtcctgat ttcgccgctg ccagtcaggc ccgtggcgtg gatttcgccg
     5821 ttgacgttca cgagcttgaa gtcgccggtg acgccttcga ccgtcacgtc gccgtcgttg
     5881 acggtcgatg cttcgaccgt tgcgccgcgc ggcacgcgga cctcgatgtc gtattcgacg
     5941 gtgtagtcgt cgccccagtt ccaatcacgc cgattgccgt cgtcatcacc ggtgcgacgc
     6001 acgcgaaacg cgacgcgccc gggctcgctc tcagtgcgca gcgcgagctc agcgcgggcg
     6061 cgctcgatat cggactgcaa gtcgccgcgc accgtctcgg tcgcgtgcat ctcgacgtga
     6121 tcgcgatcgt gctgcgtgac tcgcacggag cccgtgatgt tcttgacgat gacgacgagc
     6181 ggcgctccgg ccgtgacggg gaccgtttcg tcgagctcgc gcgtgtcgac gacgtcggcg
     6241 ctcgcgtcgc cggccgaggc caacagcgcg gcagatgccg cagctgcaat gatgcaaagg
     6301 ctcacattca tgcgttgtct cctcaaagct cggtgctatt gagcgtgctg tcggccgcga
     6361 cttcgctcaa agccatctgt g
//
LOCUS       DATLEE010000171         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_188888, whole genome shotgun sequence.
ACCESSION   DATLEE010000171 DATLEE010000000
VERSION     DATLEE010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188888"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VM692_03675"
     CDS             complement(<1..838)
                     /locus_tag="VM692_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ51295.1"
                     /translation="MSQAETQAELNPSHLHRRDLLRGAAALVAGSVALAAQAPAAAQT
                     EQQALAVDAQFFPGFRTEKVQTSGAVIHSVIGGNGPPVLLLHGAPQSRVSWAAVAREL
                     ARDYTVVVPDLRGYGDSGKIEGSTDHNEYSKRTMALDQVEVMRHYGFERFSVVGHDRG
                     ARVTHRMALDHAQRIERAALCDIMPTHYLFSRVDKAFAESAYHWFFFARPAPFPETVI
                     LNSLELFVGREDPTLGAEYRRVFRIPGTLHAMCEDYRAGASIDFEHDAADLGKKVQCP
                     LRV"
     gene            949..1401
                     /locus_tag="VM692_03680"
     CDS             949..1401
                     /locus_tag="VM692_03680"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUQ51296.1"
                     /translation="MRTTTRVLAGALAIGVAFWIGTITAQNRSPAPVRLDPDKIAGLN
                     LTAIPPDAYKEILVAGELDMRVATLFEGKELRVSIFESTPAKTDHRTRPTDVDEFVYV
                     LSGKLILTEPSGTRHEFLPGDSLVLPITYTGTWEMQGNYRELVVLAQK"
     gene            complement(1615..>2089)
                     /locus_tag="VM692_03685"
     CDS             complement(1615..>2089)
                     /locus_tag="VM692_03685"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HUQ51297.1"
                     /translation="YLAVSGVGLFYANTQGVMKRFSDITAVNGIVLSADERTLYVTNG
                     PTVAAFDVQPDGSLTNQREFAKLRSGQGGDGSAIDSEGRLYVATGGAADVFSPKGEFL
                     GTIDGPDGMHGVAFGGRDKKTLYGIVFYGGWGTPSARNQIVGIPMLAQGYTGRAK"
BASE COUNT          341 a          677 c          708 g          363 t
ORIGIN      
        1 gcacgcgtag agggcactgc actttcttgc cgaggtccgc ggcgtcgtgc tcgaagtcga
       61 tcgacgcgcc ggcgcggtag tcctcgcaca tcgcatgcag cgtgccgggg atgcggaaca
      121 cgcgccggta ttcggcgccg agcgtcgggt cctcgcggcc gacgaacagc tcgagactat
      181 tgagaatcac ggtctcgggg aacggggcgg ggcgcgcgaa gaagaaccag tgatacgcgc
      241 tttccgcgaa cgccttgtcg acgcgcgaga atagataatg agtcggcatt atgtcgcaca
      301 gcgccgcacg ctcgattcgc tgcgcgtggt cgagcgccat gcggtgcgtg acgcgcgcgc
      361 cgcggtcgtg gccgacgacg ctgaagcgct cgaagccgta gtggcgcatg acctcgactt
      421 ggtcgagcgc catcgtgcgc ttcgagtact cgttgtgatc ggtactgcct tcgattttgc
      481 cgctgtcgcc gtagccgcgc aggtcgggga cgacgacggt gtagtcgcgc gcgagctcgc
      541 gtgcgacggc agcccacgag acgcggctct gcggcgcacc gtgcaacagt aagacgggcg
      601 ggccgttgcc gccgatgacg ctgtgaatca cggcgcccga cgtctggacc ttctcggtgc
      661 gaaagccggg gaagaactgc gcgtcgacgg cgagtgcctg ctgctcggtt tgggccgcgg
      721 ccggcgcttg cgccgcgagc gcaacgctgc cggcgacgag tgcggccgcg ccgcgcaaca
      781 gatcgcgccg atgcagatgg ctcgggttca actcggcttg ggtctcggcc tggctcatgg
      841 cgattcctcg cgtcgcgacg ctgccatcgt agcgcgattt ggcgggcggc ggccgttgcc
      901 gtgctacgct ccgcggcacc gtcttaccgc gatctcgagg aaccacgaat gagaacgacg
      961 actcgtgtgc tggccggagc gctcgcaatc ggcgttgcgt tttggatcgg cacgatcacg
     1021 gcgcagaacc gcagcccggc ccccgtgcgc ctggaccccg acaagattgc gggactgaat
     1081 ctgacggcca ttccgcccga tgcctataaa gaaattctgg tcgcgggcga gctcgacatg
     1141 cgcgtcgcga cgttgttcga gggcaaggag ctgcgcgtct cgatcttcga gtcgacaccg
     1201 gccaagacgg atcaccgcac tcgcccgacc gacgtggacg agttcgtcta cgttctctcg
     1261 ggcaagctga tcctcacgga gccgagcggg acgcgccacg agttcctgcc cggcgactcg
     1321 ctcgtgttgc cgatcacgta caccggtacg tgggagatgc agggcaacta tcgcgagctc
     1381 gtcgtgctcg cgcagaagta gcgcgtcgcg tctgacggag cccgtctacg tcaccacgcg
     1441 ggtgttggtc ttcgactggt accgctcgcc gtcggtgggc gatgtcgccc acgatcgaag
     1501 ttacgacatc ctattcgtca ataagctcgc gtaccgcgac tcgtcgccgc agcgcggtga
     1561 tgtgatcgta tcgtcggtcg agttctcgtg agcgccgccg actgacgcaa ccgactactt
     1621 cgcgcggccc gtatagccct gcgccagcat cggaataccg acgatctggt tgcgcgcgct
     1681 cggcgtgccc cagccgccgt aaaacacgat gccgtaaaga gtcttcttgt cgcggccgcc
     1741 gaaggcgacg ccgtgcatgc cgtccggccc gtcgatcgtg cccaagaatt cgcccttcgg
     1801 cgagaacacg tcggccgcgc caccggtcgc aacatagagg cggccttcgg agtcgatggc
     1861 cgagccgtcg ccgccttggc cgctgcgcag cttcgcgaac tcgcgttgat tcgtcagcga
     1921 gccgtccggt tgcacgtcga acgcggcgac ggtcgggccg ttcgtgacgt agagcgtccg
     1981 ctcgtcggcg gagagcacga tgccgttgac ggccgtgatg tcgctaaagc gcttcatgac
     2041 accctgcgta ttcgcgtaga acagcccgac gccggaaacg gcaaggtag
//
LOCUS       DATLEE010000172         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_936838, whole genome shotgun sequence.
ACCESSION   DATLEE010000172 DATLEE010000000
VERSION     DATLEE010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936838"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VM692_03690"
     CDS             complement(<1..1033)
                     /locus_tag="VM692_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HUQ51298.1"
                     /translation="MDFPVKTGAPARQRTECAILPVFDDRALHGAAKDFDRAARGAIT
                     KLVRAGDAPARLGSVTLVHRTQGTAAARWLLVGCGKRADFTTKRLTTALGAAVHALRS
                     GGSKEAISYLGYETGIAAGDAARHSVETARATVYRFDELKSRTDPPSRLARLGLGYPS
                     GTGANDVRVGIKLGLAIAAGSDLARDLGNRPPNVCTPSHLAEAARNIAKRFDRMEVKV
                     LDEADMRRLGMGALLSVTQGAAEPPRMIVLQYRGAGAKQAPIALCGKGVTFDTGGISI
                     KPAPKMDEMKFDMCGAGGVLGAMNAIGELQAPVNVVALLPACENMPGGKATRPGDIVK
                     SLSGQTVEII"
     gene            1193..>2259
                     /gene="lptF"
                     /locus_tag="VM692_03695"
     CDS             1193..>2259
                     /gene="lptF"
                     /locus_tag="VM692_03695"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HUQ51299.1"
                     /translation="MNLIGRYIFREAFGSWLVVMLVLFVIFMTNQLADILGDAAADRL
                     PRDAVLAIFGLTALRYLMFLTPITLFLGVMLALARLNRDGEMAALSACGVGLTRLLVP
                     IGAFTLLLALGLTWLALVSTPAANRRVEEIRFSAEQNVELSAIEPGKFVSPDSGETVI
                     YAKEVVGDELRDVFLQTQQGDRVTVILAERGRRVVDATEEQSFMLYKGRRYEGVPGES
                     EFLRLEFDEGVLPIRPDEEEEFVELAAAKPTLELLRSSRLGDRAELQWRVSFPLQLFV
                     LSLLAVPLSRTSPREGRYARLGMALLIYLIYFDMLSIARVWMERGLIGDDVGMWWVHA
                     IAALVALLMLARAGGWLVRARP"
BASE COUNT          332 a          713 c          769 g          445 t
ORIGIN      
        1 tgataatttc gacggtttgg cccgagaggc tcttgacgat gtcgccgggc cgcgtagctt
       61 tgccgcccgg catgttctcg cacgcgggga gcagggcgac gacgttgacg ggcgcctgca
      121 gctcgccgat cgcgttcatc gcaccgagca cgccgccggc gccgcacatg tcgaacttca
      181 tctcgtccat cttcggcgcc ggcttgatcg agatgccgcc ggtatcgaag gtcacgcctt
      241 tgccgcagag cgcgatcggc gcttgcttgg cacctgcgcc gcgatactgc agcacgatca
      301 tgcgcggcgg ctcggcggcg ccttgcgtca ccgacaacaa agcgcccatg ccgagccggc
      361 gcatgtcggc ctcgtcgagc accttgactt ccatgcgatc gaaccgcttt gcaatgttgc
      421 gcgccgcttc cgcgagatgc gacggagtgc agacgttcgg ggggcggttg ccgagatcgc
      481 gcgcgagatc gctgcccgcg gcgatcgcaa gaccaagctt gatgccgacg cgcacatcgt
      541 tcgcgccggt gccgctcggg tagcccaagc ctagacgcgc gagccgcgac ggcgggtcgg
      601 tacggctctt tagctcatcg aaccgataca ccgtcgcacg cgccgtctcg acgctgtggc
      661 gtgccgcgtc gccggccgcg atgccggtct cgtagccgag atagctgata gcttccttgc
      721 tgccgccgct gcgcagcgcg tgcaccgcgg cgccgagcgc cgtcgtgagg cgcttggtcg
      781 tgaaatcggc gcgcttgccg cagccgacga gcagccagcg cgcggcggcg gttccctgcg
      841 tgcggtgtac gagagtgacg gagccgagac gcgcaggagc atcgccggcc cgaacgagct
      901 tcgtgatggc gccgcgagcg gcgcgatcga aatccttcgc ggctccgtgc aacgcgcggt
      961 catcgaacac gggcaggatc gcgcactcgg tccgctgacg tgccggtgca cccgttttga
     1021 ccggaaaatc catcctcgct ctccttcgtt cgcggcgcgt gaggtcatgc gccgcgagtc
     1081 gcggcacggc gctaaggcgg cgtcgcgctg tgctatcgtg ttgtaatact gtggaaaatc
     1141 gcagtgtagt gcattcgttt cgctgacgga actctagctt cggatcgcaa tcgtgaacct
     1201 cattggtcgc tacattttcc gtgaagcgtt cggttcgtgg ttggtggtca tgttggttct
     1261 gttcgtgatc ttcatgacga accagctcgc cgatatcctc ggcgatgccg cggcggaccg
     1321 gttgccgcgc gacgcggtgc tggcgatctt cggccttacg gcgctgcgtt atctgatgtt
     1381 cctcacgccg atcacgctgt tcttgggcgt gatgctcgcg ctcgcgcggc tgaatcgtga
     1441 cggcgaaatg gcggcgttgt cggcttgcgg cgttggcttg acgcggttgc tcgtgccgat
     1501 cggagcgttc acgttgctgt tggcgctcgg cttgacgtgg ctggcccttg tatcgacgcc
     1561 ggccgcgaac cgccgcgtcg aggaaatccg tttcagcgcc gagcaaaacg tcgagctgtc
     1621 ggcgatcgag cccggcaagt tcgtatcgcc cgactccggc gagaccgtca tctatgcgaa
     1681 agaagtcgtc ggcgacgagc tgcgcgacgt atttctgcaa acgcagcagg gcgatcgagt
     1741 cacggtgatc ctcgcggagc ggggccggcg cgtcgtcgat gcgacggaag agcaatcgtt
     1801 catgctgtac aagggccgcc gttacgaagg cgtgcccgga gagagcgaat tcctgcggct
     1861 cgagttcgac gagggcgtgc tgccgatcag gcctgatgag gaggaggagt tcgtcgagct
     1921 cgccgccgcg aaacccacgc tcgagctcct gcgttccagc cggctcgggg atcgcgccga
     1981 gctgcaatgg cgtgtgtcgt tcccgctgca actattcgtg ttgagcttgc tcgccgttcc
     2041 tttgagccgc acgagtccgc gcgaagggcg ttacgcgcgg ctcggcatgg cgctgctcat
     2101 ttatttgatc tacttcgaca tgttgtcgat cgcgcgcgtt tggatggagc gcggcctcat
     2161 cggcgacgac gtcggcatgt ggtgggtaca tgcgatcgcc gcactggtcg cgctcctcat
     2221 gttggcgcgc gcaggcgggt ggctcgtgcg cgcccgccc
//
LOCUS       DATLEE010000173         7069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9129053, whole genome shotgun sequence.
ACCESSION   DATLEE010000173 DATLEE010000000
VERSION     DATLEE010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9129053"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..457
                     /locus_tag="VM692_03700"
     CDS             53..457
                     /locus_tag="VM692_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HUQ51300.1"
                     /translation="MSGYSGTPLAKKLGIIQRATVVAIDAPKNYRRLLEPLPQSVRFA
                     SKVDKATDLVHVFSTKRAELAGLLASYRKKLAPSAAVWVSWPKKAANVPTEITEDVVR
                     EVALPLGFVDVKVCAIDETWSGLKLVVRKELR"
     gene            662..1036
                     /locus_tag="VM692_03705"
     CDS             662..1036
                     /locus_tag="VM692_03705"
                     /inference="COORDINATES: protein motif:HMM:NF025140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HUQ51301.1"
                     /translation="MKTSTLTMLAVGAVAAFAAAPVSFAQQSETVAPATEPTTVSDAD
                     LETFALIYVDLLDTVDKFRAEIEAVQTEEQALGIRERMQTESIAKVAQRGWTPEKFNS
                     IGDAINRDPGLAEKAASLIEKE"
     gene            complement(1062..1784)
                     /locus_tag="VM692_03710"
     CDS             complement(1062..1784)
                     /locus_tag="VM692_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HUQ51302.1"
                     /translation="MQGFLGELIGTMTLILLGNGVVAGVSLKGSYAENAGWIVITTAW
                     GLAVICAVIVALAVGSNAHLNPAVTIGVGIATGDWSSAPAYIAGQFLGALLGAVLVWL
                     HYLPLWRQTDNAAGKRACFCTAPAVRHIPSNVLSEIIGTFVLVFVAAASSSDNLSPNG
                     LAPGFGPFLVGLLVWGIGLSLGGMTGYAINPARDLGPRIAHALLPIAGKGSSGWDYAA
                     IPVLGPVVGAVLAGLAVVAIGI"
     gene            complement(1803..3311)
                     /gene="glpK"
                     /locus_tag="VM692_03715"
     CDS             complement(1803..3311)
                     /gene="glpK"
                     /locus_tag="VM692_03715"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HUQ51303.1"
                     /translation="MSKYVGAIDQGTTSSRFMVFDQQGAIVAVAQREHAQIYPKPGWV
                     EHDAVEIWRNTEGVIGDALTQGGIRASELAAVGITNQRETTLLWDRNTGVPLHNALVW
                     QDTRTADLVATYARDGGQDRFRARTGLPLATYFSGLKLRWLLDNVAGARAKAEAGDAL
                     FGTIDTWLLWNLTGGTAGGVHLTDVTNASRTQLIDLETLDWDDELLAAFYVPRALLPR
                     IAPSSASYGAAAIAALRGVPISGILGDQQAALVGQACFEPGEAKNTYGTGCFMLMNTG
                     PQPVPSTAGLLTTVAYQLGDEPPHYALEGSIAIAGALVQWLRDNLGLIAKSSEVEALA
                     REVQDNGDVYFVPAFSGLFAPYWRDTARGALVGLTRYANRGHIARAALEAVAYQTRDV
                     LEAMEKDSGIPIKELRVDGGMVANELLMQFQSDQLGVPVVRPRVTETTALGAADAAGL
                     AVGYWSSRTELVRNWGVERRWRPAMAEAERAKLYAAWQKAVQRSFDWVDSAS"
     gene            complement(3330..4832)
                     /locus_tag="VM692_03720"
     CDS             complement(3330..4832)
                     /locus_tag="VM692_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HUQ51304.1"
                     /translation="MTTNRRWHSGMFSTEDWWSVWIGLAIFAASLVSLAGVDLVGWAA
                     RPRAWEWTNLTGEFAWSKLFSPAGASYASWHPLVSFATTYLVFTALFATGARSLRLDV
                     RRFVVGFTVLYLVTWAAWIVGNELHLTIVDAAVDGRNRYSEAALSWGLQLGEGAPYLL
                     ALIAGLALGNFARGFAAARLTEAARPEWYIKTAIVFLGVNLGAQTIEASGFAFDLILT
                     GAAAVFVAYLFFWPIVYAVARKWFGMKRDVAAVVASAISVCGASAAIATAGAIRAKPV
                     IPVTVSMVVVIFAMIELIVLPGLYTAFLPDQPIVNGAALGMTVKTDGADAAAGALLDE
                     LMIARNLQLTGQQWQAGWILSASLLTKIWIDVFIGAWAFVLAIVWIKRVEKREGAVQV
                     SATEIWFRFPKFVFGYFIAWAVYFLLATQGEALAATLETGTAVVAGPMRTMLFMLTFV
                     AMGAITDFAKLRGMGKIALLYGLALVVVIAPIAYAVAYLFHRGITPPLAP"
     gene            complement(4829..5077)
                     /locus_tag="VM692_03725"
     CDS             complement(4829..5077)
                     /locus_tag="VM692_03725"
                     /inference="COORDINATES: protein motif:HMM:NF036574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5395 family protein"
                     /protein_id="HUQ51305.1"
                     /translation="MSVRALVLSYESSRWHARGQGVDVMHGELRGLEALIEAQLASDG
                     AAVDVRLEFDMTSLPRWLHQYHGHYCNYTLHVPARSSA"
     gene            complement(5074..5196)
                     /locus_tag="VM692_03730"
     CDS             complement(5074..5196)
                     /locus_tag="VM692_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51306.1"
                     /translation="MSGAPEPWERWETSLVLISLGVGLFALLLLGWLVDRFILS"
     gene            complement(5294..6484)
                     /locus_tag="VM692_03735"
     CDS             complement(5294..6484)
                     /locus_tag="VM692_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ51307.1"
                     /translation="MNPVTNPYAPGAGAPPPELAGRDELLERVRVAIERVRRGLPAKS
                     ILMVGLRGVGKTVWLDRMRDDAEAGGIETLRIEAPEGRSLPALLAPELRVALLKLSRN
                     EQAKALGERALRALAGFAKALKVKYHDIEVGLDLEPEPGLADNGDLEHDLAALLETVG
                     AVAQRAGTALVLFIDELQYVKEEELAALITGLHRTAQRKLPVTLVGAGLPQLRGRMGR
                     AKSYAERLFDFPAIGELAPPAARQALVKPALDEGVEITEDALDRILAETHRYPYFLQE
                     WGKHAWDTAKASPITLDDVTRASRVATAALDESFFRVRFDRLTLAEKKYLRAMAELGP
                     GPHRSGDVAEALGRKVTSLAPIRNQLIAKGMIYSPSHGDTAFTVPLFDLFLHRIMPGA
                     DWRE"
     gene            6625..>7069
                     /locus_tag="VM692_03740"
     CDS             6625..>7069
                     /locus_tag="VM692_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HUQ51308.1"
                     /translation="MAQAWKKTLARRQTLIIDGGTGTELRRRGMTLGDATWSALASLT
                     HYDLLRAIHLDYIAAGADVITTNTFATTRFVLDAAGRGDEFTQINTRAVAAAREARDS
                     SGREVALAGSISCLPPRFDVHAYPDERTEAAGYRELAETLAEAGVD"
BASE COUNT         1280 a         2401 c         2337 g         1051 t
ORIGIN      
        1 cgagtacgcg ccgctcatgg cgcgcacgac ttagcggagc aggtcgagcg ccgtgtcggg
       61 ctactcgggc acaccgctgg cgaagaagct cggaatcatc cagcgcgcga ccgtcgtcgc
      121 gatcgacgcg ccgaaaaact accgccgcct gctcgagccg ttaccgcaat cggtgcggtt
      181 cgcgtcgaaa gtcgacaagg cgacggacct cgtgcatgta ttcagcacga agcgagccga
      241 gctcgcgggc ctgctcgcga gctaccgcaa gaagctcgcc ccgagcgctg ccgtctgggt
      301 gtcgtggccg aagaaagccg cgaacgtgcc gaccgagatc acagaggacg tcgtacgcga
      361 ggtcgcgctg ccgctcggct tcgtcgacgt gaaggtctgc gcgatcgacg agacgtggtc
      421 cggtctcaag ctcgtcgtgc gcaaggagct ccgctgagcg cgccgctcag gcgggcaaga
      481 actgtagatc ggccgcatgc actgcagcga cgagctgccg tcgccgcaac cgcggcgctg
      541 cgatcgctgg aacgaccggc acggatgttg cagccctggg cgcccggccg cggagctgga
      601 cgtccgcgcg acgtgctcga ttcgggccgc tttgcagaac tgacaacagg aggatccaag
      661 gatgaaaacc tcgacactga cgatgttggc cgttggggcc gttgccgcgt ttgcggccgc
      721 acccgtctcg tttgcgcagc aatccgaaac cgtggcgccg gcgaccgagc ccacgacggt
      781 ttccgacgcg gatctcgaga cgtttgcgtt gatctacgtc gacctgctcg acaccgtcga
      841 caagttcaga gcggagatag aagcagtgca aacggaagag caggccctcg gcatccggga
      901 gcgaatgcag acggagagca tcgcgaaggt cgcgcagcgc ggttggaccc cggagaagtt
      961 caacagcatc ggcgatgcaa tcaatagaga cccggggctg gccgagaagg ccgcatcgct
     1021 gatcgagaaa gaatagcgcg cgcgaggcgc gcgtcagcgc gtcagatgcc gatcgcgacg
     1081 acggcgaggc ccgcgagcac ggcgccgacg acggggccga gcacggggat cgccgcgtaa
     1141 tcccagcccg agctgccttt gccggcgatc ggcagcaacg cgtgcgcgat gcgcggaccg
     1201 agatcgcgcg ccgggttgat cgcgtagccc gtcatgccgc cgagcgacaa gccgattccc
     1261 cagacgagca gcccgacgag gaacggaccg aagcccggcg ccagaccgtt cggcgataga
     1321 ttgtccgagg aactcgccgc ggcgacgaac acgagcacga acgtgccgat gatctcgctg
     1381 agcacgttcg acgggatgtg ccgcacggcc ggcgcagtgc agaagcacgc gcgcttgccg
     1441 gcggcgttgt cggtctggcg ccagagcggc aagtagtgca gccagacgag cacggcgccg
     1501 agcaacgcgc ccaagaattg cccggcgata taggccggcg cgctgctcca gtcgcccgtc
     1561 gcgatgccga cgccgatcgt caccgcgggg ttcagatgcg cgttgctgcc gacggccagc
     1621 gcgacgatga cggcgcagat gacggcgagc ccccaggccg tcgtgatgac gatccagccg
     1681 gcgttctcgg catacgagcc tttcaacgac acgccggcga cgacgccgtt gccgagcaag
     1741 atcagcgtca tcgtgccgat gagctcgcct aggaaacctt gcactcgcgc tctccttcgc
     1801 gatcaactcg cgctgtcgac ccaatcgaac gagcgctgca cggctttctg ccacgcggcg
     1861 tacagcttgg cgcgctcggc ttcggccatc gcggggcgcc aacgccgttc gacgccccag
     1921 ttgcgcacga gctccgtgcg gctcgaccaa taaccgacgg cgaggcccgc ggcatccgcc
     1981 gcgccgagcg ccgtcgtctc tgtcacgcgc ggccgcacga cgggcacgcc gagctgatcc
     2041 gattggaact gcatcaaaag ctcgttcgcg accatgccgc cgtcgacgcg cagctccttg
     2101 atcgggatgc cggagtcttt ctccatggct tcgagcacgt cgcgcgtttg atacgcgacg
     2161 gcctcgagcg ccgcgcgcgc gatgtggccg cgattcgcat agcgcgtgag gccgacgagc
     2221 gcgccgcgcg cggtgtcgcg ccaatacggc gcgaacagtc ccgagaacgc cgggacgaag
     2281 tagacgtcgc cgttgtcctg cacctcgcgc gcgagcgctt cgacttcgga gctctttgcg
     2341 atcaacccca agttgtcgcg cagccattgc acgagcgcgc cggcgatcgc gatcgagcct
     2401 tcgagcgcgt agtgcggcgg ctcgtcgccc aattgataag cgacggtcgt gagcaagccc
     2461 gccgtcgacg gcaccggctg cgggccggtg ttcatcagca tgaagcagcc cgtgccgtag
     2521 gtgttcttcg cctcgccggg ctcgaagcac gcttggccca cgagcgcagc ctgctggtcg
     2581 ccgaggatgc ccgagatcgg cacgccgcgc agcgcggcga ttgcggccgc gccgtagctc
     2641 gcgctcgacg gcgcgatgcg cggcaacagc gcgcgcggca cgtaaaacgc cgcgagaagc
     2701 tcgtcgtccc agtccagcgt ctcgaggtcg atcagctgcg tgcggctcgc attcgtcacg
     2761 tcggtcaaat gcacgccgcc ggccgtgccg cccgtgaggt tccagagcag ccaggtgtcg
     2821 atcgtgccga acaacgcgtc gccggcctcg gccttcgcgc gtgcgcccgc aacgttgtcg
     2881 agcagccagc gcagcttgag cccgctgaaa taggtcgcga gcggcaagcc cgtgcgcgcg
     2941 cgaaagcgat cttggccgcc gtcgcgcgcg taagtcgcga cgaggtccgc cgtccgcgtg
     3001 tcctgccaca cgagtgcgtt gtgcagcggc acgccggtgt tgcggtccca aagcaaagtc
     3061 gtttcgcgtt gattcgtgat gccgacggcc gcgagctcgc tggcgcgtat gccgccctgc
     3121 gtgagggcgt cgccgatgac gccttccgtg ttgcgccaga tctcgacggc gtcgtgctcg
     3181 acccagcccg gcttcggata aatttgcgca tgctcgcgct gcgcgacggc gacgatcgct
     3241 ccttgctggt cgaacaccat gaaacggctg ctcgtcgtgc cttgatcgat cgcgccgacg
     3301 tacttactca tgctcgcgcg ctcctcagat caaggcgcca acggcggcgt aatgccgcgg
     3361 tggaacaaat aggcgaccgc gtaggcaatc ggcgcgatca cgacgaccag cgcgaggccg
     3421 tacagcagcg cgatcttccc catgcccctg agcttcgcga aatccgtgat cgcacccatc
     3481 gcgacgaacg tcagcatgaa cagcatcgtg cgcatcgggc ccgccacgac ggccgtgccg
     3541 gtctcgagcg tcgccgccag cgcctcgccc tgcgttgcga gcaggaagta aacggcccac
     3601 gcgatgaaat agccgaacac gaacttcggg aagcgaaacc aaatctccgt cgcgctgact
     3661 tgcaccgcgc cttcgcgctt ctcgactctc ttgatccaaa cgatcgcaag cacgaacgcc
     3721 caggcgccga tgaacacgtc gatccagatc ttcgtcaaca gcgaggcgct caggatccag
     3781 cccgcctgcc attgctggcc cgtcaactgc agattgcgcg cgatcatgag ctcgtcgagc
     3841 agcgcgccgg ccgcggcatc ggcgccgtcc gtcttgacgg tcatgccgag cgcggcgccg
     3901 ttgacgatcg gctgatcggg caagaacgcc gtatagaggc ccggcaggac gatcagctcg
     3961 atcatcgcga agatgacgac gaccatcgag acggtgacgg gaatgacggg cttcgcgcga
     4021 atcgcgccgg ccgtcgcgat ggcggccgaa gcgccgcaga cggaaatcgc cgaggccacg
     4081 accgcggcca cgtcgcgctt catgccgaac cacttgcgcg ctacggcata gacgatcggc
     4141 cagaaaaaca aatacgcgac gaacacggcc gcggcgcccg tcagaatgag atcgaacgcg
     4201 aagccgctgg cctcgatcgt ctgcgcgccc aagttgacgc cgagaaacac gatggccgtc
     4261 ttgatgtacc actcgggccg cgcggcctcg gtcaaacgtg ccgcggcgaa accgcgcgcg
     4321 aagttgccga gcgccaagcc ggcgatcaac gcgagcagat acggcgcgcc ttcgccgagc
     4381 tgcaagcccc agctcaaggc cgcttcggag tagcgattgc gaccgtcgac ggcggcgtcg
     4441 acgatcgtca ggtgcagctc gttgccgacg atccaagcgg cccacgtcac gaggtacagc
     4501 accgtaaaac cgacgacgaa gcggcgcacg tcgagccgaa gagagcgggc gcccgtcgcg
     4561 aacagcgccg tgaacacgag atacgtcgtc gcgaacgaca cgagcggatg ccagctcgcg
     4621 tagctcgcgc cggccggcga gaacaacttg ctccaggcga actcgcccgt caggttggtc
     4681 cactcccaag ctcgaggccg cgctgcccag ccgacgaggt cgacgccggc taacgagacc
     4741 aagctcgcag cgaagatcgc aaggccgatc caaaccgacc accaatcctc ggtgctgaac
     4801 atgccgctgt gccagcgccg gttcgtcgtc atgcgctcga gcgcgccggc acgtgcagcg
     4861 tgtagttgca gtagtgaccg tgatactgat gcagccagcg cggcaacgac gtcatgtcga
     4921 actcgaggcg gacgtcgacg gcagcgccgt cactcgcgag ctgggcctcg atcagcgcct
     4981 cgaggccgcg cagctcgccg tgcataacgt cgacgccctg gccgcgcgcg tgccaacgac
     5041 tgctttcgta ggacagcacg agcgcgcgca cgctcatgac aagatgaagc gatcgacgag
     5101 ccagccgagc agcaataacg cgaaaagacc gacgcctaag ctaatcagca cgagcgaggt
     5161 ttcccaacgc tcccacggct ccggcgctcc ggacaatgcg tcttcgcttg gcggcgcctg
     5221 tggcgatccg tcctgcatat cgaccccccg atttcgcaat ctgtgttgcg tgcgtcgaga
     5281 gtattagcac gttctactcg cgccagtcag cccccggcat gattcggtgc agaaacaggt
     5341 cgaacaacgg cacggtgaac gccgtgtcgc cgtggctcgg gctgtagatc atgcctttcg
     5401 caatcagctg gttgcggatc ggcgccagcg aagtgacttt gcgcccgagc gcttcggcga
     5461 cgtcgcccga gcgatgcggg cccgggccaa gctctgccat cgcgcgcaaa tatttcttct
     5521 ccgcgagcgt cagccgatcg aaccgcacgc ggaagaagct ttcgtcgagc gcggccgttg
     5581 cgacgcgcga cgcgcgcgtg acgtcgtcga gagtgatcgg cgacgccttc gccgtgtccc
     5641 acgcgtgctt gccccactcc tgcagaaaat acgggtaacg atgcgtctcc gcgaggatgc
     5701 ggtcgagcgc gtcctcggtg atctcgacgc cttcgtcgag cgcgggcttc acgagcgctt
     5761 gccgcgcagc cggcggcgcg agctcgccga tcgccggaaa atcgaacaag cgttcggcgt
     5821 acgacttggc tcgccccatt cgcccgcgca gctgcggcaa gcctgcgccg acgagcgtga
     5881 cgggtaactt gcgttgcgca gtccggtgca aacccgtgat cagcgcggcc agctcttctt
     5941 ctttgacgta ctgcagctcg tcgatgaaca gcacgagcgc cgtgccggca cgttgcgcca
     6001 cggcgccgac ggtctcgagc aacgcggcca agtcgtgctc gaggtcgccg ttgtcggcga
     6061 gcccaggctc gggctcgagg tcgagcccga cctcgatgtc gtgatacttg accttcaacg
     6121 ccttggcgaa gcccgcgagg gcgcgcagcg cgcgctcgcc gagcgccttc gcctgctcgt
     6181 tgcgcgacag cttgagcagc gcgacgcgca gctccggcgc cagcagcgcc ggcaacgagc
     6241 ggccctcggg tgcctcgatg cgcagcgtct cgatcccgcc cgcctcggcg tcgtcgcgca
     6301 tacggtcgag ccagacggtc ttgccgaccc cgcgcaggcc gaccatgagg atgctcttcg
     6361 cgggtaagcc gcggcggacg cgctcgatcg cgacgcggac ccgctcgagg agctcatcgc
     6421 gtccggccag ctcgggcggc ggcgcaccgg cgcccggagc gtagggatta gtgacggggt
     6481 tcataggcag attgtaggga atttagtgaa gttatcaaat atcagtaact tcaataaact
     6541 ccccataact gaatccgagg cccaggtggc ggatagcgcg atcgttcgag gcgtaacgga
     6601 ttgcggcagc cgtacgataa cggtatggcg caggcatgga agaagaccct cgcgcgccgc
     6661 cagacgctga tcatcgacgg tggcacgggc acggagctgc ggcggcgtgg aatgacgctc
     6721 ggcgacgcca cgtggagcgc gctggcttcg ttgacgcact acgacctgct gcgcgcgatc
     6781 catctcgact acatcgcggc cggcgccgac gtcatcacga ccaacacgtt cgcgacgacg
     6841 cgcttcgtgc tcgacgcggc ggggcgcggc gacgagttca cgcagatcaa cacccgggcc
     6901 gtcgcggccg cccgcgaagc acgcgactcg agcggccgcg aagtcgcgct cgcgggctcg
     6961 atctcgtgct tgccgccgcg gttcgacgtg cacgcatacc ccgacgagcg gaccgaggcc
     7021 gcggggtatc gcgagctcgc ggagacgctc gccgaagccg gcgtcgatc
//
LOCUS       DATLEE010000174         3546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3079587, whole genome shotgun sequence.
ACCESSION   DATLEE010000174 DATLEE010000000
VERSION     DATLEE010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3079587"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1708)
                     /locus_tag="VM692_03745"
     CDS             complement(218..1708)
                     /locus_tag="VM692_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HUQ51309.1"
                     /translation="MSEQWKKSSARFGWLAGAAGVGVLSFSLLTVNQHSSAQPAAPRA
                     PAAVGVSFADVIESVSPAVVNISVMKVESAAPTSFQYSVPRGQGQLPFDEFFERFFDG
                     ERGGQMPERRSEGQGSGFLIDASGYIVTNNHVAGGADQITVTLQDGRKFEAKLVGNDP
                     KTDLAVVKVEASGLPYVAFGDSDKARVGDWVVAIGNPFGLGGTATAGIVSARGRDINS
                     GPYDDYLQLDAPINFGNSGGPVFNTAGEVVGVNTAIFSPNGGNIGIGFAIPANQAKEI
                     VADLRENGSVERGWLGVQIQNVDEEIAKSLHLKGTEGALVADVVADGPAQHAGVQQGD
                     VITKFNGRDVDSSRTLSRLVASASPNEKSKVTVWRDGRSHELTVDLGEAEQDEAVAAT
                     EPHGPAQSSAAVGMSLRPLTNDDRASLGLPSGVNGAIVASVQPGSAAAEKGIRPGDVI
                     TRVNQKAVTSVADAVAALNSARDRNETALLLVRRGDAQQFVALSFS"
     gene            1915..2319
                     /locus_tag="VM692_03750"
     CDS             1915..2319
                     /locus_tag="VM692_03750"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HUQ51310.1"
                     /translation="MSKSFIAKIAAVGLAAGLTVGSSASFARSADSQIDANQPAFEQV
                     DSIPMLTRPHSWQVLDEDSVIVWATPFDPYLVELAFKSHDLKFAEVIGITQFGSRVYA
                     RFDAVKVGGFRYPIDSIYKMTREEARNLVRGT"
     gene            2403..3080
                     /locus_tag="VM692_03755"
     CDS             2403..3080
                     /locus_tag="VM692_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006917871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUQ51311.1"
                     /translation="MRILVIEDDKDVASFIRNGLAQAGCNVDHAANGKDGLFLATTES
                     YDALVVDRMLPGIDGLTVIRTLRASANTTPVLILSALGEVDDRVKGLKAGGDDYLVKP
                     FAFSELLARLEALARRAKSGADAPTTTLKVGDLEMDLLRREVRRGGKPIDLQPREFQL
                     LEFLLRHAGQVVTRTMLLEGVWDYHFDPQTNVIDVHISRLRSKIDRGFDRPLLHTVRG
                     AGYRLDD"
     gene            3094..>3546
                     /locus_tag="VM692_03760"
     CDS             3094..>3546
                     /locus_tag="VM692_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HUQ51312.1"
                     /translation="MQLPRLFRTRVFRLTLAYMALFALSVGALSAFIYWATIGYLETQ
                     TNAIIEAEINGLYEQYERSSVRGLADVIDERVRRDTEGRSFYMLANPIGQYIAGNVTS
                     WPRGIDDAQAQWADFVQPDSTTPVRAMLLRVGSFRLLVGRDIREQVAIR"
BASE COUNT          618 a         1165 c         1120 g          643 t
ORIGIN      
        1 cctatcagta gaaagtgcac gagcggatct ttgatgagct tcgacattcg ctggcgatgg
       61 gcgcgctgaa aaacggccag ggattatggc cgcgcgccgc gagaggtcac aagctgcggt
      121 gcggcgcagg cggaaaaaaa gggtcccggc cagagagggg ggagggggat tggccgggac
      181 ccttacgaac gaatgccgct agtacgaaat gcgttgtcta cgagaagctc aaggccacga
      241 actgttgcgc gtcgccgcga cgcacgagca gcaatgccgt ctcgttgcga tcacgcgccg
      301 agttcagcgc ggcgacggcg tcggcaacgc tcgtgacggc cttctgattg acgcgcgtga
      361 tgacgtcgcc ggggcgaatg cccttctccg ccgcggcgct gccgggctgg accgacgcca
      421 cgattgcgcc gttgacgccg ctcggcagcc cgagcgaggc acggtcatca ttcgtcagcg
      481 ggcgcaagct catgcccacg gccgcgctgc tctgcgccgg accatgcggc tccgtcgccg
      541 cgacggcttc gtcctgctcg gcctcgccga gatcgaccgt cagctcgtgg ctgcggccgt
      601 cacgccacac cgtcaccttc gacttctcgt tcggtgacgc agaggcgacg agtcggctca
      661 gcgtacgcga cgagtcgacg tcgcggccgt tgaacttcgt gatcacgtca ccttgctgca
      721 cgcctgcgtg ctgtgcgggg ccgtccgcga cgacgtcggc gacgagcgcg ccttcagtgc
      781 ccttcaggtg caacgacttc gcaatttcct cgtcgacgtt ctggatctgc acgccgagcc
      841 agccgcgctc cacgctgccg ttctctcgca gatccgcgac gatttccttc gcctggttgg
      901 ccggaatcgc gaacccgatt ccgatgttgc cgccgttggg cgagaagatt gccgtgttca
      961 cgccgacgac ttcgcctgcc gtgttgaaca cggggccgcc cgagttgccg aagttgatcg
     1021 gcgcgtcgag ctgcaagtag tcgtcgtacg ggcccgagtt gatgtcgcgg ccgcgggccg
     1081 agacgatacc ggccgtcgcc gtgccgccga ggccgaacgg attgccgatc gcgacgaccc
     1141 aatcgccgac gcgcgccttg tcggagtcgc cgaaggcaac atacggcaag ccgctggctt
     1201 cgactttcac gaccgcgagg tccgtcttcg gatcgttgcc gacgagcttc gcttcgaact
     1261 tgcggccgtc ctgcaacgtc accgtgatct ggtcggcgcc ccccgccacg tgattgttcg
     1321 tgacgatata gccgctcgcg tcgatcaaga agcccgagcc ttgcccttcg ctgcggcgct
     1381 ccggcatctg gccgccgcgc tcgccgtcga agaaccgctc gaagaactcg tcgaacggca
     1441 attgtccttg cccacgcggc accgagtact gaaacgacgt cggcgcggcg ctctcgacct
     1501 tcatcacgga tatgttgacg acggccgggc tcacactctc gatgacgtcg gcaaatgaga
     1561 cgccgacggc agcgggtgca cgcggggccg ccggttgcgc actcgagtgt tgattcaccg
     1621 tcaataacga aaacgacagc acgcctacac cggcggctcc agcgagccag ccgaaacgtg
     1681 cggaactctt cttccactgt tcggacatcg cagctctcct tccgaggttt ggttggttca
     1741 tggctgccac gttaatgccg gggggcttac gtctagctca acggatccct acggttttgt
     1801 aatgaaacct agctgcggcg ctgaatcctg ctgaatcgtt aagtttgcgt tcagctgtgt
     1861 gcgaatagcc ttgcgccata gtccaacgca gcagtgtttc taaggagaac aaccatgagt
     1921 aagtcattca tcgccaaaat cgcggccgtg ggcctcgcgg cgggtttgac ggtcggctcg
     1981 agcgcaagtt tcgctcgcag cgcggacagc caaatcgacg ccaaccagcc cgcgttcgag
     2041 caagtggaca gcatcccgat gctgactcga ccgcacagct ggcaggtgct cgacgaggat
     2101 tccgtgatcg tctgggcgac gccgttcgac ccgtacctcg ttgaactcgc gttcaagagc
     2161 cacgatctga agttcgccga ggtcatcggc atcacgcagt tcggtagccg cgtgtatgcg
     2221 cgattcgatg cggtcaaggt gggtggcttc cgctacccta tcgacagcat ctataagatg
     2281 actcgcgaag aggcccgcaa tttggtgcgc ggcacctgag gtcagtagcg ggtctcggcc
     2341 ttccctcccc ccggccgaga cccgcgtacg ctcgggcgca cgccgagcgt atggtgtgac
     2401 acgtgcgaat tctcgtcatc gaggacgaca aggacgtcgc ttccttcatc cgcaacggat
     2461 tggcgcaagc cggctgcaac gtagaccacg ctgccaacgg taaggacggc ctgttcctcg
     2521 cgacgaccga gagctacgac gcgctcgtcg tcgaccgcat gctgcccggc attgacgggc
     2581 tcaccgtcat ccgcacgctg cgcgcgtccg cgaacacgac gcccgtcttg atcttgagcg
     2641 cgctcggcga ggtcgacgat cgcgtcaaag gcctcaaggc cggcggcgac gactacctcg
     2701 tcaagccgtt cgccttcagc gagttgctcg cgcgcctcga ggcgctcgcg cgccgtgcca
     2761 agagcggcgc cgacgcgccg acgacgacgt tgaaggtcgg cgatctcgag atggatttgc
     2821 tgcggcgcga ggtgcgccgc ggcggcaaac cgatcgacct acaaccgcgc gaattccaat
     2881 tgctcgagtt tctgctgcgc cacgcgggcc aagtcgtgac gcgcacgatg ttgctcgaag
     2941 gcgtgtggga ctatcacttc gacccgcaga cgaacgtcat cgacgtgcac atcagccgct
     3001 tgcgctcgaa gatcgaccgc ggtttcgacc ggccgctgct gcacaccgtg cgcggcgccg
     3061 gttaccgcct cgacgactga cgcgctcatc cgcatgcagc tgccgcgcct gtttcgcacc
     3121 cgcgtgttca gactcacgct cgcgtacatg gcgttgttcg cgctgtccgt cggtgccctg
     3181 tcggcattca tatactgggc gacgatcggt tatctcgaaa cgcagaccaa cgcgatcatc
     3241 gaggccgaga tcaacggtct ttacgagcaa tacgagcgca gcagcgtgcg gggcttggcc
     3301 gacgtcatcg acgaacgcgt gcggcgcgac accgaagggc gctccttcta catgctcgcg
     3361 aatccgatag gccagtacat cgcgggcaac gtgacgtctt ggccgcgcgg catcgacgac
     3421 gcgcaagcgc agtgggccga cttcgtgcag ccggacagca cgacgccggt ccgcgcgatg
     3481 ctgctgcgcg tcggctcgtt ccggctgctc gtcgggcgcg acattcgcga gcaagttgcg
     3541 atcaga
//
LOCUS       DATLEE010000175         2280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_940013, whole genome shotgun sequence.
ACCESSION   DATLEE010000175 DATLEE010000000
VERSION     DATLEE010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940013"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..498)
                     /locus_tag="VM692_03765"
     CDS             complement(106..498)
                     /locus_tag="VM692_03765"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51313.1"
                     /translation="MKRKLLILAGAAALMSGIAYGHHSFTATYDEAKTVKIEGELVQF
                     MFRNPHAWVHVMAPDENGEMQRWGVEWGGAGALTGQGVTRESLKPGDKVVITGNPGRN
                     PIDHRVRMRSLLRPSDGFGWGFQGETFD"
     gene            complement(524..1630)
                     /locus_tag="VM692_03770"
     CDS             complement(524..1630)
                     /locus_tag="VM692_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51314.1"
                     /translation="MSHDVLPGLVRRAACAFAIAAALAVTGAPTADAQQRGAQRGGET
                     AGELHWWPVRKNLWMLVGAGTNIAASVGPDGVLLVNAGNAQSSARVVTAVKDLQTQLN
                     AFGFLDVNTPQRGGAETRSRFPVNSHPPPKPVRYIIDTSSLPHSIGGNQAVAASGVTY
                     TGGNVAGTIADSSEGAAVLAHENVLNRMVQADAPFAALPTETYFTGEYKVSTFFNDEG
                     VQIMHAPNAATDGDSWVYFRGSDVIAAGDLVNMDSFPIIDVEAGGSINGVLDGLNAIL
                     KLAIPEFRTEGGTMIVPGHGRLADSADVGYYRDMLTIIRDQVKALIDEGLTLEQVKER
                     RPTFGYEARFGKNTGTWTTDMFIEAVYRSLKENS"
     gene            complement(1633..>2280)
                     /locus_tag="VM692_03775"
     CDS             complement(1633..>2280)
                     /locus_tag="VM692_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51315.1"
                     /translation="RPHSAAYIKRGPSNLRVWKEVDPASRAIVAFQTEWLRSTNTPIY
                     MDGRPHPPEYAAHTWGGFSTGSWEGDRLRIDTTHMKEDYYRRNGVPQSDRAKLTEYLM
                     RRRFQNEDYLTWVIVAYDPVYLTEPLIRSTEYRLNLAQQVPPYPCTIVTEVVRPEGYV
                     PNWLPGKNDQIYSFSDFYGLPRELYFDGAETMYPEYHKKLERLGATAARQAPGRE"
BASE COUNT          351 a          732 c          773 g          424 t
ORIGIN      
        1 gcgccggttg cgcggcgccg cgacgtgcct gcgcgccggc gccggtcgcg agcgcaacgc
       61 acgcggccaa cgcgacaccg gtcaataacg agagtcgcag catgttcaat cgaaggtctc
      121 gccttggaag ccccagccga agccgtcgga gggccgcagc aggctgcgca tgcgcacgcg
      181 gtgatcgatc gggttacgcc ccgggttgcc cgtgatgacg actttgtcgc cgggcttcag
      241 cgattcacgc gtgacgcctt ggcccgtcag cgcgccggca ccgccccatt cgacgcccca
      301 gcgctgcatc tcgccgttct cgtcgggcgc catgacgtgc acccacgcgt gcgggttgcg
      361 gaacatgaac tgcacgagct cgccctcgat cttgacggtc ttggcttcgt catacgtcgc
      421 ggtgaacgag tgatgcccgt aggcaattcc cgacatcaac gcggcagcgc cggcgaggat
      481 gagtagtttg cgtttcacgt ggtgacctcc cgatgaacca gagctatgaa ttctctttca
      541 agctgcgata gacggcttcg atgaacatgt cggtcgtcca agtgcccgtg ttcttgccga
      601 agcgcgcctc gtaaccgaac gtcggccgtc gctccttgac ttgctcgagc gtgagcccct
      661 cgtcgatcag cgctttgacc tgatcgcgaa tgatcgtgag catgtcgcgg tagtagccga
      721 cgtcggccga gtcggcgagg cggccgtggc ccggcacgat catcgtgccg ccctcggtgc
      781 ggaactccgg tatcgcgagc ttcaggatcg cattcaaccc gtcgagcacg ccgttgatgc
      841 tgccgccggc ctcgacgtcg atgatcggaa acgaatccat gttgacgaga tcgccggctg
      901 cgatgacgtc ggagccgcgg aagtagaccc agctgtcgcc gtcggtcgcc gcgttcggtg
      961 cgtgcatgat ctgcacgcct tcgtcgttga agaacgtgct gactttgtac tcgcccgtga
     1021 agtacgtttc cgtcggcagt gcggcgaacg gcgcgtccgc ttggaccatg cggttcaaca
     1081 cgttctcgtg tgcgagaacc gcggcgcctt ccgacgagtc cgcgatcgtg ccggcgacgt
     1141 tgccgcccgt gtacgtgacg cccgaggccg cgacggcttg gttgccgccg atgctgtgcg
     1201 gcagcgagct cgtgtcgatg atgtagcgga cgggcttcgg cggcggatgc gagttcaccg
     1261 ggaagcgtga gcgggtctcg gcgccgccac gctgcggcgt gttgacgtcg aggaagccga
     1321 atgcgttcag ctgcgtctgc agatccttga ccgcggtcac gacgcgcgcg ctcgattgcg
     1381 cgttgccggc attcacgagc agcacgccgt cgggcccgac cgacgcggcg atgttcgtgc
     1441 ccgcgccgac gagcatccag aggttcttgc ggaccggcca ccagtggagc tcgccggcgg
     1501 tctcgccacc gcgctgcgcc ccgcgttgct gggcgtcggc ggtcggtgcg ccggttacgg
     1561 ccaatgcggc ggcgattgca aacgcgcagg cggcgcggcg cacgaggccg ggaagcacgt
     1621 cgtgactcat ccttactctc gccccggcgc ctgtcgtgcg gccgtggctc ccaagcgctc
     1681 gagcttcttg tgatattcgg ggtacatcgt ctcggcgccg tcgaagtaca gctcgcgcgg
     1741 taaaccgtag aagtcggaga agctgtagat ctgatcgttc ttgccgggca gccaattcgg
     1801 cacgtagccc tcgggccgca cgacctcggt cacgatcgtg caggggtagg gcggtacttg
     1861 ctgcgcgaga ttgagccggt actccgtgct gcggatcagc ggctcggtca gatagacggg
     1921 gtcgtacgcg acgatgaccc aggtcaggta gtcctcgttc tgaaaccggc gccgcatcaa
     1981 gtactcggtc agcttcgcgc ggtcgctttg cggcacgccg ttacggcggt agtagtcttc
     2041 cttcatgtgc gtcgtatcga tgcgcaggcg gtcgccttcc cacgagcccg tcgagaaccc
     2101 gccccacgtg tgggccgcat actcgggcgg gtgcggccgg ccgtccatat aaataggagt
     2161 gttggtcgac cgcagccact cggtctgaaa cgcgacgatg gcgcgcgagg ccgggtcgac
     2221 ttctttccag acgcgcaagt tcgacgggcc gcgcttgatg taggcggcgg aatgcgggcg
//
LOCUS       DATLEE010000176         2677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9131729, whole genome shotgun sequence.
ACCESSION   DATLEE010000176 DATLEE010000000
VERSION     DATLEE010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9131729"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VM692_03780"
     CDS             <1..282
                     /locus_tag="VM692_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HUQ51316.1"
                     /translation="AAALSTLGSSATLTWQPPALNTDGTPLTDLSAFKVYWGTTQGTY
                     PYSAQISNVAARTHTVTGLQTGQWYFVVTALNSKGVESPKSNVWTKTVP"
     gene            complement(305..1051)
                     /gene="modA"
                     /locus_tag="VM692_03785"
     CDS             complement(305..1051)
                     /gene="modA"
                     /locus_tag="VM692_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HUQ51317.1"
                     /translation="MVRSLLATVALLVASLGTRTATADEIRVAVATNFSETMAALVER
                     FEQDSEHTVLVSAGSTGNLYAQIRNGAPFEVFFSADAERPMLLEKQGVALEGSRFLYA
                     VGRLVLWSPRPGLVDADGRVLESGTFKFLAIANPELAPYGAAAREVLMARNLWSALEG
                     RLVQGQDIGQAYSFVHTGNAELGFVAYSQLKQPGSEIAGSYWLVPESLHRPIAQEAVL
                     VRDVPAARAFVEFVKSAPAREIIRRYGYGP"
     gene            complement(1060..1135)
                     /locus_tag="VM692_03790"
     tRNA            complement(1060..1135)
                     /locus_tag="VM692_03790"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1100..1102),aa:Thr,seq:cgt)
     gene            complement(1213..1623)
                     /locus_tag="VM692_03795"
     CDS             complement(1213..1623)
                     /locus_tag="VM692_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HUQ51318.1"
                     /translation="MTRDEAATLLTGRGVLPTAQRVDIALLTLARPQHLSAEQIIATI
                     RGSGLRISKATVYNTLNLFCEKGLLRTVDVDPTRQFYDSSTAAHHHFYNVDTGELTDI
                     PLESVMLQVDTALPPGTEHAGVDVVVRVRSSRAD"
     gene            1783..2316
                     /locus_tag="VM692_03800"
     CDS             1783..2316
                     /locus_tag="VM692_03800"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HUQ51319.1"
                     /translation="MRFALGFTLWELLCTLGIAAVAFGAGVPAFTSFLLDARLTADVN
                     GWVLAVQLARSEAYKRGQPVVVCRTVDTQRCAGAAPAAAGWMVFVNLDDESPPQRSST
                     EPLLYVHAPEVIGSIVGNRPYYELRPGRRSTNGTTVFCDRRGAAAAKAVVVSYTGRPR
                     VDTRDGDGLALRCPAQP"
     gene            2415..>2677
                     /locus_tag="VM692_03805"
     CDS             2415..>2677
                     /locus_tag="VM692_03805"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HUQ51320.1"
                     /translation="MNTSRQHGFTLWELLMTLLVAGILLGIGVPNVMEFQRNGAMTAA
                     ANTLVTGLLMARNEAVKRQAPVTVCLSDNPTAVAPTCAPNAVA"
BASE COUNT          467 a          891 c          873 g          446 t
ORIGIN      
        1 gccgcagcgc tgtcgacgtt gggcagctcc gcgactctga cgtggcagcc gccggccttg
       61 aacacggacg gcacgccgct cacggacctg tcggcattca aggtttactg gggcacgacg
      121 cagggcacgt acccctactc cgcgcagatc agtaacgtcg ctgcacgcac gcacaccgtg
      181 acggggctcc agaccggcca gtggtacttc gtcgtgacgg cgctgaactc caagggcgtc
      241 gaaagcccga agtcgaacgt ctggaccaag accgtcccgt agacacgggg gccgtcagga
      301 caagtcaggg cccgtaaccg tagcggcgaa tgatttcgcg cgcgggggcg ctcttgacga
      361 actcgacgaa cgcgcgcgcg gccggcacat cgcgcacgag caccgcctct tgcgcgatcg
      421 gccggtgcag cgactccggc acgagccaat aagagcccgc gatctcgctg cccggctgct
      481 tgagctgcga ataggcaacg aagccgagct ccgcgttgcc cgtgtgaacg aagctgtagg
      541 cctggccgat gtcctggccc tgcacgaggc gcccttcgag cgcgctccac agatttcgcg
      601 ccatcaagac ctcgcgcgcc gcggcgccgt agggcgcgag ctccggattc gcgatcgcga
      661 ggaacttgaa cgtgccgctc tcgagaacgc gcccgtcggc gtccacgagc cccggccggg
      721 ggctccaaag cacgagccgt ccgacggcgt ataaaaagcg cgagccttca agcgcaacgc
      781 cttgtttctc cagcaacatt ggacgctcgg cgtccgcgga gaaaaagacc tcgaacggcg
      841 cgccgttgcg aatctgcgcg taaagattgc ccgtcgagcc ggcgctgacg agcaccgtat
      901 gctcgctgtc ttgctcgaat cgctcgacca acgcagccat cgtttcgctg aaattcgttg
      961 cgactgcgac gcggatctcg tcggccgtcg ccgttcgagt ccctagactc gcgacgagca
     1021 gtgcaacggt cgccagcagg cttcgaacga tgaggtaggt ggtgccggtg gagggactcg
     1081 aacccccgac cccccgctta cgaagcggat gctctaccaa ctgagctaca ccggcaaaaa
     1141 tggaaggcgc gtattgtgcc tgagctcggc acgtggcgca cgcaacaata atgtatgacg
     1201 cgcgctcggt cgtcagtcgg cgcgactgct ccgcaccctg acgacgacgt cgacgccggc
     1261 atgctcggtg cccggcggca gtgccgtatc gacctgcaac atcacggact cgaggggaat
     1321 gtccgtgagc tcgcccgtgt cgacgttata gaaatggtga tgcgcggccg tggacgagtc
     1381 gtagaactga cgcgtcggat cgacgtcgac cgtgcgcagc aagcccttct cgcaaaacag
     1441 gttcagcgtg ttgtagacgg tcgccttcga gatgcgcagg ccgctgccgc ggatcgtcgc
     1501 gatgatctgc tccgccgaaa gatgctgcgg ccgagccaac gtcagcagcg cgatgtcgac
     1561 gcgctgcgcg gtcggcaaca ctccgcggcc cgtaagtagg gtggcggctt cgtcacgtgt
     1621 catcgttttc gattccatac aaatcagcga cttggggagt atacgcgcga gccggcgtcg
     1681 atatcaaagc gtgcgcctta acttgacgta gtcgcaacat ttcgtggtcg atacgccggg
     1741 cccccggcga tagcgtcgct cgagcagacc aaggagggtc tcatgcgatt cgcgctcggg
     1801 ttcacgctct gggagctgct ttgcacgctc ggcatcgccg ccgtcgcgtt cggtgccggc
     1861 gtccccgcct tcacctcgtt tttgctggat gcgcggctta cggccgacgt caacggttgg
     1921 gtattggccg tgcagttggc ccgcagcgag gcctacaaac gtggccagcc cgtcgtggtg
     1981 tgccgcaccg ttgacacaca gcgttgcgcg ggcgccgcgc ccgcagcggc gggctggatg
     2041 gtgttcgtca atctcgacga tgagtcgccg ccgcagcgtt cgagcacaga gccgctgctg
     2101 tacgtgcatg cgcccgaggt catcggctcg atcgtcggca atcgccctta ctacgagctc
     2161 cggcccggcc gtcgcagcac aaacggcacg accgtattct gcgatcggcg cggcgccgcg
     2221 gccgcaaagg ccgttgtcgt cagctatacg gggcggccgc gcgtcgatac gcgcgacggg
     2281 gatggcctgg cgctacgctg ccctgcgcag ccttgacata acgcggcggc gacaacggcc
     2341 gctcatccga cggccgctgc cggtcgtcgg gcggagccct ggggcgcggc gccggctccg
     2401 tatagtccgc ggccatgaac acgtctcgcc agcacggatt cacgctttgg gagctcctga
     2461 tgaccctgct cgtcgcgggc atcctgctcg gcatcggcgt accgaacgtc atggagtttc
     2521 agcgcaacgg cgccatgact gccgcggcga acaccttggt gaccggtttg ctgatggcgc
     2581 gcaacgaagc cgtgaagcgc caagcgcccg tgacggtctg cttgagcgac aacccgaccg
     2641 ccgtcgcgcc gacttgcgcg ccaaacgccg tcgcgaa
//
LOCUS       DATLEE010000177         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9135444, whole genome shotgun sequence.
ACCESSION   DATLEE010000177 DATLEE010000000
VERSION     DATLEE010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9135444"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="VM692_03810"
     CDS             <1..1549
                     /locus_tag="VM692_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HUQ51321.1"
                     /translation="KLTIMRNEMASEVNVMARDAARVARQNPRTADFTHGLLRTALRE
                     IIANFPVYRTYVDAAGHCTEADLAHFGEALAGARRRAAALDPSVFTFLARLLTGKLVE
                     APRSGFSRTAVLRCAMKLQQLSGPVMAKGLEDTAFYRYNRFIALNEVGGEPQRIGSSV
                     DDFHAANRERARRWPHALSATSTHDTKRGEDARARLAVLTELPDEWAEQARSWRDALH
                     AGAHEVQPDANDEYYFYQSLLGAWPPELCDARELDERALGELATRLRGAMTKAVREAK
                     RHSTWAAPDERYEGAVATLVDSALSSQAVLAQLAPFASRIARLGVRNSLVQLALKLTA
                     PGVPDVYQGTETWDLSLVDPDNRRRVDFAHRAKLLESVEEGLAADRVRSLERWLESWQ
                     DGRIKLATLRLLLALRARAAELFALGTYEACAVEGPRAAEVVAFVRRHERSVVLTAVQ
                     RFPVRAERSSDWRGTRIAAPPDAEGMVDVVTGRKVGADLDPRELFATLPIAVLEPERR
                     HEEEARG"
     gene            1527..2216
                     /locus_tag="VM692_03815"
     CDS             1527..2216
                     /locus_tag="VM692_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311993.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HUQ51322.1"
                     /translation="MKKKRAADDARADEGDEGSPHGAADFLPKTPSLPAARAAAAGCR
                     GCGLYRHATQTVFGEGAPRAKVMMIGETPGDREDLAGRPFVGPAGQLLDRALAAAQVD
                     RKTVYVTNVVKHFKWEPRGKRRLHKRPMPGEIAACLPWLELEIDVVRPKVIVCLGATA
                     GQALFGSAFSVTRERGRFLASRFAPRVFATLHPSALLRVREQAARHAAFDAFVADLEL
                     IDDALRGKRAS"
BASE COUNT          334 a          802 c          841 g          346 t
ORIGIN      
        1 caagctcacg atcatgcgca acgagatggc gagcgaggtc aacgtgatgg cgcgcgatgc
       61 cgcgcgcgtt gcgcggcaaa atccgcgcac ggccgacttc acgcacggcc tgctgcgcac
      121 ggcgctacgc gagatcattg cgaacttccc cgtctatcga acctatgtcg acgccgccgg
      181 gcattgcacg gaagccgatc tcgcgcattt cggcgaggcg ctcgccggcg cgcggcgccg
      241 cgccgccgcg ctcgacccga gcgtgttcac gtttctcgcg cggctgctga caggcaagct
      301 cgtcgaggcg ccgcgcagcg gcttcagccg cacggccgtc ctgcgctgtg cgatgaagct
      361 gcagcagttg agcgggcccg tcatggccaa aggcctcgag gacacggcgt tctaccgcta
      421 caaccgcttc atcgcgttga acgaggtcgg cggcgagccg cagcgcatcg gcagcagcgt
      481 cgacgacttt cacgcggcga accgcgaacg cgcgcggcgc tggccgcacg cgctgtcggc
      541 gacctcgacg cacgacacga agcgcggcga ggacgcgcgc gctcgactcg ccgtgttgac
      601 cgagctgccc gacgaatggg ccgagcaagc gcgcagctgg cgagacgcgt tgcacgcggg
      661 cgcgcacgag gtgcagcccg acgccaacga cgaatactac ttttatcaat cgttgctcgg
      721 cgcgtggccg cccgagcttt gcgacgcgcg tgagctcgac gagcgggcgc tcggcgagct
      781 tgcgacgcga ctgcgcggcg cgatgacgaa ggccgtgcgc gaagcgaaac gacattcgac
      841 gtgggcggcg cccgacgagc gttacgaagg cgctgtcgcg acgctcgtcg actcggcgct
      901 gtcgtcgcag gccgtcctgg cccagcttgc gccgttcgcg agccgtatcg cgcggctcgg
      961 cgtgcgcaac agcttggtgc agctcgcgtt gaagctgacg gcgcccggcg tgcccgacgt
     1021 ctatcaaggc acggagactt gggacttgag cctcgtggac cccgacaacc gccggcgcgt
     1081 cgacttcgcg caccgcgcga agctgctcga gtccgtcgag gagggcctgg ctgccgatcg
     1141 cgttcgttcg ctcgagcgct ggctcgagtc ttggcaagac gggcgcatca agctcgcgac
     1201 gctgcgcctg ctgctcgcgc tgcgcgcgcg cgcagcggag ttgtttgctc tcgggactta
     1261 tgaggcgtgc gcggttgagg ggccgcgcgc cgcggaggtc gtcgcctttg tgcgccggca
     1321 cgagcgcagc gtcgtgctga cggccgtgca gcgcttcccg gtgcgcgcgg agcgcagcag
     1381 cgattggcgc ggcacgcgaa tcgctgcacc gcccgatgcc gagggcatgg tcgacgtggt
     1441 gacgggccgc aaagtcggcg ctgacctcga cccccgcgag ctgttcgcga cgctgccgat
     1501 cgccgtgctc gagccggagc gccgtcatga agaagaagcg cgcggctgac gacgcgcgag
     1561 ccgacgaggg cgacgaaggg tcgccgcacg gtgcggccga tttcttgccg aagacgccgt
     1621 cgctgccggc agcgcgtgcg gcggccgccg gctgccgcgg ctgcgggctg taccggcacg
     1681 cgacgcagac cgtgttcggt gagggtgcgc cacgcgccaa ggtcatgatg atcggcgaga
     1741 cgccgggcga ccgcgaggat ctcgcgggcc ggccgttcgt cggcccggcg ggccagttgc
     1801 tggaccgcgc gctcgccgcc gcgcaggtcg accgcaagac cgtctacgtg acgaacgtcg
     1861 tcaagcactt caaatgggag ccgcgcggca aacgccgctt gcacaagcgg ccgatgcctg
     1921 gcgagatcgc agcctgtctg ccgtggttgg agctcgagat cgacgtcgtg cggcctaaag
     1981 tcatcgtctg tctcggcgca accgccggcc aggcgctgtt cggcagcgcc ttttcggtaa
     2041 cgcgcgagcg cggccggttc ctggcgtcgc gctttgcgcc gcgcgtgttc gcgaccctgc
     2101 atccgtcggc gctgctgcgc gttcgcgagc aggccgcgcg gcacgccgcg ttcgacgcct
     2161 tcgtcgccga tctcgagctc atcgacgacg ccttgcgcgg gaagcgggcg tcatgatgct
     2221 cgccgacctc gcgcaggtct ctgccgcggt tgccgcgacg cgcagccgcg gcgagaaaac
     2281 gcgtttgctc gccgagttgc tgcgctcgct ggcgcccgac gag
//
LOCUS       DATLEE010000178         2386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_944043, whole genome shotgun sequence.
ACCESSION   DATLEE010000178 DATLEE010000000
VERSION     DATLEE010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944043"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VM692_03820"
     CDS             <1..1238
                     /locus_tag="VM692_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HUQ51323.1"
                     /translation="DARTGRLLWTFHTIPEKGEFGYDTWLTPGSAEYTGNAGAWGPLS
                     ADPELGLVYLPVEGATSDMYGGERHGNNLFGNSLVAVDVKTGERRWHQQLVHHDIWDW
                     DNPTSPILLDVNVGGRPVKAVVQLTKQAFAYAFDRATGEPLWPIEERPVAKSDVPFEW
                     TSPTQPFPTRPAAYDRQGVGESDLVDFTPAIKAAALEAVKDLRMGPMFTPPSLIQAPD
                     GTRGTLVLPHYGGGANWEGGAADPETGMLYVASQTSLTVFAVNAAGDRSDIRYLFVNG
                     EAPRPMGLPLVKPPYGRITAIDMNTGDHRWMAVNGDTPDDIKNNPALAGVTIPPTGKA
                     SRATLLVTKTLLFAGEGYGGAPLMRAYDKATGAVVAEIPLPGAASSKPMTYMQGGKQY
                     IVLAVGRDTPAELVAFALQ"
     gene            1249..2193
                     /locus_tag="VM692_03825"
     CDS             1249..2193
                     /locus_tag="VM692_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUQ51324.1"
                     /translation="MTSGYAIELRGVTKSFGNFTAVDALDLAIPRGALYGFIGPNGAG
                     KTTTIRMIMSILLPDAGTVNVLGASALDAKDRIGYLPEERGLYRKMKVSPYLSYIARL
                     KGVPERGLRQKVSAALERVGLAGVEGKRCEELSKGMSQKIQIIAATIHEPDLLILDEP
                     FSGLDPVSTRLLREQILAEHERGATVLFSTHVMPQAEDICDHVVMVHRGKKVLDDPVS
                     TILRPQDSRVLKLELLDVKADVAGVRALPDVESCSAVDDGYEVTLLPGADATRAMQAV
                     LATVPVMRIELKRPTLEDVFVGIVAGDEPADARQGVAA"
     gene            2190..>2386
                     /locus_tag="VM692_03830"
     CDS             2190..>2386
                     /locus_tag="VM692_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51325.1"
                     /translation="MNKVLQIAVREFVATVFTKGFIIALLIVPAIFGLMAVFGPRLFG
                     NATNLAVEGEIAVVDATGVVLP"
BASE COUNT          412 a          807 c          801 g          366 t
ORIGIN      
        1 tcgacgcgcg tacgggccgt ttgctctgga cgttccacac gatccccgag aaaggggagt
       61 tcggctacga cacgtggctg acgcccggct ccgccgagta cacaggcaat gccggcgcgt
      121 ggggcccctt gtcggccgat cccgagcttg ggctcgtcta cctgcccgtc gaaggcgcga
      181 cgagcgacat gtacggcggc gagcgccacg gcaacaactt gttcggcaac agcctcgtgg
      241 ccgtcgacgt gaaaaccggc gagcgccgct ggcatcagca gctcgtgcat cacgacatct
      301 gggactggga caacccgacc tcgccgatcc tgctcgacgt caacgtcggc gggcggcccg
      361 tcaaagccgt cgtgcagctc acgaagcagg cgttcgccta cgcattcgac cgcgcgaccg
      421 gcgagccgct gtggcccatc gaggagcgcc ctgtcgcgaa atccgacgtg ccgttcgaat
      481 ggacttcgcc gacgcagccg ttcccgacgc ggccggccgc ttacgatcga cagggcgtcg
      541 gcgaaagcga tcttgtcgac ttcaccccgg cgatcaaggc tgcggcgctc gaggccgtca
      601 aagatctgcg catggggccg atgttcacgc cgccgtcgct gattcaagcg cccgacggca
      661 cgcgcggcac gctcgtgctg ccgcactacg gcggcggcgc gaactgggag ggcggagcgg
      721 ccgaccccga gacgggcatg ctgtacgtcg cttcgcagac gagcctcacg gtgttcgccg
      781 tgaacgcggc cggcgatcgc tccgacatcc gttacctgtt cgtcaacggc gaggcgccgc
      841 ggccgatggg cttgccgctc gtgaagccgc cgtatggccg catcacggcc atcgacatga
      901 acacgggcga ccaccgctgg atggccgtca acggcgacac gcccgacgac atcaagaaca
      961 accccgcgct cgcgggcgtg acgatcccgc cgacgggcaa agcgagccgc gcaacgttgc
     1021 tcgtcacgaa gacgctgctg ttcgccggcg aaggttacgg cggcgcgccg ctcatgcgcg
     1081 cctacgacaa agcgaccggc gccgtcgtcg ccgagattcc gctgccgggc gccgcgagca
     1141 gcaagccgat gacgtatatg cagggcggca agcaatacat cgtgctcgcc gtcggccgcg
     1201 atacgccggc cgagctcgtc gcattcgcgt tgcaataggg gatcgacgat gaccagcggt
     1261 tacgcgatcg agctgcgcgg cgttacgaag tcgttcggca acttcacggc cgtcgacgcg
     1321 ctcgatctcg cgataccacg cggcgcgctg tacggcttca tcgggccgaa cggcgccggc
     1381 aagacgacga cgatccgcat gatcatgtcg atcctactgc cggacgcggg caccgtgaac
     1441 gtgctcggcg cctcggcgct cgacgcgaaa gaccgcatcg gctacctgcc ggaggagcgc
     1501 ggcctttatc gcaagatgaa ggtctcgcca tacctgtcgt acatcgcgcg cttgaagggt
     1561 gtgccggaac gcggcttgcg ccaaaaggtt tccgctgcgc tcgagcgagt cggcctcgcg
     1621 ggagtcgagg gcaagcgctg cgaggagctg tcgaaaggca tgtcgcagaa gatccagatc
     1681 atcgcggcga cgattcacga gcccgacctg ttgatcctcg acgagccgtt cagcggcctc
     1741 gaccccgtca gcacgcgcct gctgcgcgag caaatactgg ccgagcacga gcgcggagcg
     1801 acggtgctgt tctcgacgca cgtcatgccg caggccgaag acatctgcga tcacgtcgtc
     1861 atggtccatc gcggcaaaaa agtcctcgac gatcccgtga gcacgatcct gcggccgcaa
     1921 gattcgcgcg tgctgaagct cgagctgctc gacgtcaagg ccgacgtcgc tggcgtgcgc
     1981 gcgctgcccg acgtcgaaag ctgctcggcc gtcgacgacg gctacgaggt cacgctgctc
     2041 cccggcgcgg acgcgacgcg cgcgatgcag gccgtgctgg ccaccgttcc cgtcatgcgc
     2101 atcgagctca aacggccgac gctcgaggac gtgttcgtcg gcatcgtggc gggcgacgag
     2161 ccggccgacg cgcggcaagg agtggcggca tgaacaaggt tctgcagatc gccgtgcgcg
     2221 agttcgtcgc gacggtgttc acgaaaggct tcattatcgc gctgttgatc gtgccggcga
     2281 tcttcgggct catggccgtg ttcgggccgc ggctgttcgg caacgcgacg aacctcgccg
     2341 tcgaaggcga gatcgccgtc gtcgacgcga ccggtgtcgt gctgcc
//
LOCUS       DATLEE010000179         2453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3924367, whole genome shotgun sequence.
ACCESSION   DATLEE010000179 DATLEE010000000
VERSION     DATLEE010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3924367"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /gene="tesB"
                     /locus_tag="VM692_03835"
     CDS             complement(<1..859)
                     /gene="tesB"
                     /locus_tag="VM692_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HUQ51326.1"
                     /translation="MRPILADLLELLELERIEDRIFRGESRDLGGARVFGGQVLGQAL
                     TAASYTVQARHVHSLHAYFLVGGDVNAPIVYEVDVARDGKSFSNRRVVAIQHGQPIFN
                     MTASFQVPEEGLEHAATMPQVPGPDGLPDVRELAPDVLAKVPEKMRRFLTHERPFEFR
                     PVEPIHVLAPPRGAPVRHIWVKTVDALPDNADLHRNVLAYVSDYQLVSTATLPHGIHF
                     AEGNVQLASLDHAMWFHRPFRADEWLLYAMESPNASGGRGLALGKFFTRDGRLVASTA
                     QEGVVRVWAG"
     gene            complement(866..1744)
                     /locus_tag="VM692_03840"
     CDS             complement(866..1744)
                     /locus_tag="VM692_03840"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ51327.1"
                     /translation="MANVAFLGLGVMGFPMAGWLARKGHTVTVYNRTAAKAERWCTQY
                     QGKAAATPAAAAQGAEIVFSCVGDDPDLRDIALGSGGVIAAMKSGAIFCDHTTASADV
                     AREIAAAGKKRGVHCLDAPVSGGQAGAENGKLTVMVGGDAEAFATAEPIIRSFAQAVR
                     LIGPAGSGQLTKMVNQIAIAGVVQGLSEALNFARKAGLDVPTVIDTISKGAAQSWQME
                     NRWKTMNDGKFDFGFAVEWMRKDLRICLQEARNNGAELPVAALVDQFYADIVGMGGKR
                     WDTSSLIARLNRNEKK"
     gene            1860..2237
                     /locus_tag="VM692_03845"
     CDS             1860..2237
                     /locus_tag="VM692_03845"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51328.1"
                     /translation="MSSTILKKGIASLALLLPIANGSAHHSPVAFKTDVTDFVLEGDI
                     EQVEMRNPHSVMQLRVTNDDGSTTRWDIEFSSVNLLLRRGWDFDRIKVGDRVKCVGNP
                     SATGRSEMYMWSIVLPDGTEFGR"
     gene            2234..>2453
                     /locus_tag="VM692_03850"
     CDS             2234..>2453
                     /locus_tag="VM692_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51329.1"
                     /translation="MTVRRVAAMLACGLCIAAHCTAQEEPAIGGIWLPDASRSERFPA
                     QPPYTDEGRRIVTEWRASHDPLEDDPGKF"
BASE COUNT          432 a          832 c          792 g          397 t
ORIGIN      
        1 ggccagccca gactcgcacg acgccttcct gcgccgtcga cgcgacgagc cggccgtcgc
       61 gcgtgaagaa tttgccgagc gcgaggccgc gaccgcccga ggcgttcggg ctctccatcg
      121 cgtacagcag ccactcgtcg gcacggaacg gccggtggaa ccacatcgcg tggtcgaggc
      181 tcgcgagctg cacgttgccc tcggcgaagt gaatgccgtg cggcagagtc gccgtcgaca
      241 cgagctgata atcggaaacg taagcgagca cgttacggtg caggtcggcg ttgtcgggca
      301 gcgcgtcgac ggtcttgacc caaatgtgcc ggaccggcgc gccgcgcggc ggagcgagca
      361 cgtgaatcgg ctcgacgggg cggaactcga acggccgctc gtgcgtgaga aaacggcgca
      421 tcttctcggg cactttcgcg agcacgtcgg gcgcgagctc gcgaacgtcg ggtaaaccgt
      481 cggggccggg cacctgcggc atcgtcgccg cgtgctcgag gccctcctcc ggcacttgga
      541 acgacgccgt catgttgaag atcggctggc cgtgctgaat cgcgacgacg cgccggttgc
      601 tgaagctctt gccgtcgcgc gcgacgtcga cctcgtagac gatcggcgcg ttgacgtcgc
      661 cgccgacgag gaagtacgcg tgcagcgaat gcacgtggcg ggcttggacc gtatagctcg
      721 cggccgtcag cgcttgaccg agcacttggc cgccgaaaac acgcgcgcct cccaagtcgc
      781 gactctcgcc gcgaaagatc cgatcctcga tgcgctcgag ctcgagcagc tccaagaggt
      841 cggcgaggat cggccgcacg agccgctact tcttctcgtt gcgattcaaa cgcgcgatca
      901 agctcgaagt atcccagcgt ttaccgccca tgccgacgat atcggcatag aactgatcga
      961 cgagcgccgc gacagggagc tcggcgccgt tgttgcgcgc ttcttgcaag cagatgcgca
     1021 agtccttgcg catccattcg acggcgaagc cgaaatcgaa cttgccgtcg ttcatcgtct
     1081 tccagcgatt ctccatctgc cacgattggg cggcgccctt cgagatcgta tcgatgacgg
     1141 tcggcacgtc gaggccggcc ttgcgagcga agttcagcgc ctcggagagg ccctgcacga
     1201 cgccggcgat cgcaatctgg ttgaccatct tcgtgagctg gcccgagccc gcggggccga
     1261 tcaggcgcac ggcttgcgcg aacgagcgaa tgatcggctc cgccgtcgcg aacgcctcgg
     1321 cgtcgccgcc gaccatgacc gtcagcttgc cgttctcggc gccggcttga ccgcccgaga
     1381 ccggcgcgtc gaggcagtgc acgccgcgct tcttgccggc ggccgcaatc tcgcgcgcga
     1441 cgtcggccga agccgtcgtg tgatcgcaga agatggcgcc cgacttcatt gcggcgatga
     1501 cgccgcccga cccgagtgcg atgtcgcgca agtccggatc gtcgccgacg cagctgaaca
     1561 cgatctcggc gccttgtgcc gccgccgctg gagtcgcggc ggctttgccc tggtactgcg
     1621 tgcaccagcg ctcggctttc gcggccgtgc ggttgtacac ggtgaccgtg tggcccttcc
     1681 gcgcgagcca gcccgccatc ggaaaaccca tgacgcccaa accgagaaac gctacgttcg
     1741 ccatcgtctt caattcctgt cgtatgcgca cacccgcggc agcgcagcgg gcacagcata
     1801 cctttctcgg gcaggatttt ggtgccagac tccgcgcgga ttcacgggca aggagaccca
     1861 tgagcagcac gatcctaaaa aagggcatcg cgtcgctggc gctgctccta ccgatagcca
     1921 acggatcggc tcatcactcg ccggtcgcgt tcaaaacaga cgtcaccgac ttcgtgctcg
     1981 agggcgacat cgagcaagtc gagatgcgca acccgcactc cgtcatgcag ctgcgcgtga
     2041 ccaacgacga cggctcgacg acgcgctggg acatcgagtt cagcagcgtc aatctgctat
     2101 tacgtcgcgg ttgggacttc gaccgcatca aagtcggcga ccgagtgaaa tgcgtcggca
     2161 atcccagcgc gacgggccgt tccgagatgt acatgtggtc catcgtcctg cccgacggca
     2221 cggagttcgg ccgatgaccg tgcgacgcgt cgccgccatg ctcgcctgcg gcctgtgcat
     2281 cgcagcacac tgcacggccc aggaagagcc ggcaatcggc ggcatctggc tacccgacgc
     2341 gagccgcagc gagcgttttc cggcgcaacc gccgtacacc gacgaaggcc ggcgcatcgt
     2401 cacagaatgg cgcgcgagcc acgatccgct cgaggacgac cccggcaagt tct
//
LOCUS       DATLEE010000180         4672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9790929, whole genome shotgun sequence.
ACCESSION   DATLEE010000180 DATLEE010000000
VERSION     DATLEE010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9790929"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VM692_03855"
     CDS             complement(<1..1009)
                     /locus_tag="VM692_03855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HUQ51330.1"
                     /translation="MSRDPIERGLEIEQAQLRGFARTVAEVEWLLLILVVLYLFIGNP
                     ALARSVGVLSTLLGFAGFVLVFRYARPFAKHTHFRLTLEVFAMLAFLTAVLVASGGVH
                     SPLVNLFLLPIVTAALALGKRAAALVVALVCLCYPLVGMLTDGPDSLNGDFAIAAVGV
                     LAPFVLVGFSTTLLVDNINTAKSRIRALSDRDELTSLYNMRAFARLAEQEHDLAGRMA
                     RAYSVLMIDIEHLKIVNDTYGHEAGSRAVKLVAEALLRLTRSTDVVARYGGDEFALIA
                     PGGGGEVVGRRVRDALALIEVGGEPVSVRVGAVRYPDDGATSDELLTAAGSAVEEAKR
                     RG"
     gene            complement(1048..2301)
                     /locus_tag="VM692_03860"
     CDS             complement(1048..2301)
                     /locus_tag="VM692_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HUQ51331.1"
                     /translation="MALTLRGVIVLLTGIVFGLGLALGGTVWTSFSAHANAKATPAPL
                     DPTIESAALVAEVIDRVRREYVDKVDDKRIVEAAIRGIVADLDQYSTFLDTEEYEDIR
                     ISTTGSYTGIGLDVNLEDGKVTVVNPLEGAPAERAGILPGDVVAAVNDVPVDSADVEA
                     SVSRMRGAPGTAVTLDVLRPGSEEPLRFALTRSEVHVRTVQSEYLGNGLAYVRLSSFA
                     EGSAADLEHAAHDLIAESGRSDLLGMVLDLRSNPGGLLDSAVDIADTFLSEGIIVSGT
                     GRIRQAQFEQVATAGDVLENVPMVVLVNSASASASEIVAGALQDHGRARIVGETTYGK
                     GSVQTVMPLGEGSAIKLTTSRYLTPSGRSINGSGIEPDVVVHSDDPHRQYRGAGGRVP
                     LRDDAQLLEAIKLISYDSITLSSVP"
     gene            complement(2394..3575)
                     /locus_tag="VM692_03865"
     CDS             complement(2394..3575)
                     /locus_tag="VM692_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HUQ51332.1"
                     /translation="MRTVEPSQRRRLARLGCAALATVVLGSLGLAQDRRESEAALSTV
                     RKEIKGLQERMARETARRDESSRALRGAEVEIAAATRELIKIRDDLRAQQTARRELTQ
                     ETERANRRLGAEKAALARQVRSSYMTGREELFKLLLSQESPAALGRMLVYFDYYNRAR
                     TARIDTVAGDLEKLGSLERERAHLELELAALEAAQAREVSALTRSRDERRAAVAKLDA
                     EIRDNGAAVAKLRTEEQRLAELMTRLSEVLAGFPVDTDEPFSGLKGKLAWPVQGKLAG
                     DFGQPRGAGPVKWNGVLLEASAGTPVRAVYHGRVAFADWLQGLGLLVIVDHGGGDMSL
                     YGHNEALLKESGDWVEPGEAIAQVGDTGGQARAGLYFEIRYNGEPINPHPWIGRRPAA
                     P"
     gene            3639..4055
                     /locus_tag="VM692_03870"
     CDS             3639..4055
                     /locus_tag="VM692_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HUQ51333.1"
                     /translation="MAQLVEFVGHHVWLMAGLVLASAAVMIYEVRLRAQGATHVSAAD
                     AVRLINKGALVIDVRKPEEFETGHIVNARNVPAERVQQGDEIHKKKDKILLAVCTDGA
                     SSGRAAGQLRKAGYENAFSLKGGLAGWRADNLPLVK"
     gene            4057..4329
                     /gene="grxC"
                     /locus_tag="VM692_03875"
     CDS             4057..4329
                     /gene="grxC"
                     /locus_tag="VM692_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018304517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HUQ51334.1"
                     /translation="MADERVVVYSQPFCGYCSAAKRLLTAKGAAFTEIDVMFEAGRRD
                     EMIERSGGRRTVPQIFIDGRHIGGYDDLSALDKAGELDGLLSSTAP"
     gene            4363..>4672
                     /locus_tag="VM692_03880"
     CDS             4363..>4672
                     /locus_tag="VM692_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HUQ51335.1"
                     /translation="MAQEQPTPAPQKQLLLQKIYVKDLSFESPKAPMVFTTNVQPQTQ
                     LNVRSNAQQIAQDTQEVTLTITVEAKDNNTTLFLAEVAQSGIFVIQGYTAEEQSIIVG
                     S"
BASE COUNT          819 a         1562 c         1541 g          750 t
ORIGIN      
        1 ggccgcgccg cttcgcctcc tcgaccgccg aacccgcagc cgtgagcagc tcgtcgctcg
       61 tcgcgccgtc gtcggggtag cgcaccgcgc cgactcgaac gctgacaggc tcgccgccga
      121 cttcgatcaa cgccagcgca tcgcgcacgc gtcgtccgac gacctcgccg ccgccccccg
      181 gggcgatcag agcgaattcg tcgccgccat agcgcgcgac gacatccgtc gagcgcgtca
      241 atcggagcag cgcttcggcg acgagcttga ccgcacggct gccggcttcg tgaccgtacg
      301 tgtcgttgac gatcttcaag tgctcgatgt cgatcatgag cacggaatag gcgcgcgcca
      361 ttcggcccgc caggtcgtgc tcctgctcgg cgagtcgcgc gaacgcccgc atgttgtaca
      421 gcgacgtgag ctcgtcgcga tccgacaacg cacggatgcg gctcttcgcc gtgttgatgt
      481 tgtcgacgag cagcgtcgtc gagaagccga cgaggacgaa cggcgcgagg acgccaactg
      541 ccgcaatcgc gaaatcgccg ttcaggctat ccggcccatc cgtcagcatg ccgacgagcg
      601 gataacagag gcagacgaga gcgacgacga gcgcagccgc ccgcttgccg agcgccagcg
      661 ccgccgtgac gatcggcagc aagaacaaat taacgagtgg gctgtgcact ccgccggacg
      721 ccacgagcac ggcggtcagg aacgccagca tcgcgaaaac ctcgagcgtc agcctgaagt
      781 gcgtgtgctt agcgaacggg cgcgcataac gaaacaccag cacgaagccg gcgaaaccga
      841 gcagggtgct gagcacaccg acgctgcgcg cgagcgctgg gttgccgatg aagagataca
      901 acacgacgag gatgaggagc agccactcga cctcggcgac ggtgcgagcg aaaccgcgaa
      961 gttgagcctg ctcgatctcg aggccacgct cgatggggtc gcgactcatg gcaccacgtt
     1021 aaccgcggcg cgcgcccgcg tcgagcgtca cgggacggag ctgagcgtga tcgagtcgta
     1081 gctgatgagt ttgatggctt cgagcaattg cgcgtcgtcg cgcagcggca cgcgcccgcc
     1141 ggcgccgcgg tattggcgat gcggatcgtc gctgtgcacg acgacgtcgg gctcgatgcc
     1201 gctgccgttg atcgaacggc ccgacggcgt caggtaccgc gacgtcgtca gcttgatcgc
     1261 actgccttcg ccgagcggca tgacggtctg caccgaacct ttgccgtagg tcgtctcgcc
     1321 gacgatgcgt gcacggccgt ggtcctgcag cgcgccggct acgatctccg aggccgatgc
     1381 ggaagccgag ttcacgagca cgaccatcgg cacgttctcg agcacgtcgc cggcggtcgc
     1441 gacttgctcg aattgagctt ggcggatgcg ccccgtgccg ctgacgatga tgccttcgga
     1501 gagaaacgta tcggcgatgt cgacggccga atcgagcaga ccgcccggat tgctgcgcag
     1561 atcgagcacc atgccgagca aatcggaacg acccgactcg gcgatcaagt cgtgcgcggc
     1621 gtgctcgaga tcggcggcgg agccttcagc gaacgatgac agccgcacgt aggcgagacc
     1681 gttgccgagg tattcgctct gcacggtccg gacgtggacc tcgctgcgcg tcaacgcgaa
     1741 ccgcaacggc tcctcgctgc cgggccgcag cacgtcgagc gtgaccgcgg tgccgggggc
     1801 gccgcgcatg cgcgacacgg aagcctcgac gtcggcggaa tcgacgggca catcgttgac
     1861 cgcggcgacg acgtcacccg gcaggatacc ggcgcgctcg gccggtgccc cctcgagcgg
     1921 gttgacgacg gtgactttac cgtcctcgag gttcacgtcg agcccgatgc cggtgtagct
     1981 gcccgtcgtg ctgatgcgga tgtcctcgta ctcctcggta tcgaggaacg tggagtactg
     2041 gtcgaggtcg gccacgatgc cgcggatggc ggcttcgacg attcgcttgt cgtcgacctt
     2101 gtcgacgtat tcgcggcgca cacggtcgat gacctcggcg acgagcgctg cggactcgat
     2161 cgtggggtcg agcggtgccg gcgtcgcttt ggcgttggcg tgcgccgaga agctcgtcca
     2221 gacggtgccg ccgagcgcca gcccgagccc gaaaacgatg ccagtgagca gaacgattac
     2281 gccgcgaaga gtcagggcca tttcgttttt attagatccc ccggaagacc ccggcacctt
     2341 aacacagcga gaagcgcgcg caaccgccgc gacttatgtt catcgcgtag gcgtcagggc
     2401 gccgccggac gccgcccaat ccatgggtgc gggttgatgg gctcgccgtt atagcgaatt
     2461 tcgaagtaca accccgcgcg cgcttgacca ccggtatcgc cgacctgcgc gatggcctcg
     2521 cccggttcca cccagtcgcc ggactccttg agaagcgctt cgttgtgccc gtacaggctc
     2581 atgtcaccgc cgccgtgatc gacgatgacg agtaacccga gcccttgcag ccagtcggcg
     2641 aacgcgacgc ggccgtgata gacggcgcgc accggcgtgc cggcgctggc ctcgagcagc
     2701 actccgttcc atttgacggg cccggccccg cgcggctgac cgaagtcacc ggcgagcttg
     2761 ccttgcacgg gccacgcgag cttgcccttc aggcccgaga acggctcgtc ggtgtcgacg
     2821 ggaaaccccg cgagcacctc gctcaaacgc gtcatgagct cggcgagccg ctgctcctcg
     2881 gtgcgcagct tggcgacggc cgcgccgttg tcgcgaatct cggcgtcgag cttcgcgacg
     2941 gccgcgcggc gctcatcgcg cgaccgcgtc agtgccgaga cttcgcgagc ttgcgcggcc
     3001 tcgagtgccg cgagctccag ttcgaggtgc gccctctccc gctcgagcga gccgagcttc
     3061 tcgaggtcgc cggctacggt gtcgatgcgc gccgtgcgcg cgcgattgta gtaatcgaag
     3121 tagacgagca tgcggcccag cgccgcgggg ctttcttgcg acagcagcag cttgaatagc
     3181 tcctcgcggc cggtcatgta gctcgagcgg acttgccgcg ccagcgcggc tttctcggca
     3241 ccgaggcggc gattcgcgcg ctcggtctct tgcgtcagct cgcgccgcgc cgtctgctgc
     3301 gcacgcaggt cgtcgcgaat cttgatgagc tcgcgcgtgg ccgctgcgat ctccacctcc
     3361 gccccacgca gcgctcgcga gctttcatcg cggcgcgccg tctcgcgcgc catgcgctct
     3421 tgcagccctt tgatctcttt gcggaccgtc gacagcgccg cttcgctttc gcgtcgatct
     3481 tgcgcaagcc cgagcgagcc caagacgacg gtcgccaacg cggcgcagcc gagccgggcg
     3541 aggcggcgcc gttgcgatgg ctcgactgtt cgcatacggg gccgcagtgt agccgatcgc
     3601 cggccgcgcg agccgccgct gctatactgg ccggccttat ggctcagctc gtcgaattcg
     3661 tcggccacca tgtttggctc atggctgggc tcgtgctcgc gtcggcggcc gtgatgattt
     3721 acgaggtccg gcttcgtgct caaggcgcga cgcacgtctc ggccgcggac gccgtgcggc
     3781 tcatcaacaa aggcgcgctc gtcatcgacg tgcgcaagcc cgaggaattc gagaccggcc
     3841 atatcgtcaa tgcgcgcaac gtgcccgccg agcgggtgca acagggcgac gaaatccaca
     3901 agaagaagga caagattctg ctggccgtgt gcaccgacgg cgcgagctcg ggtcgggcgg
     3961 ccggacaact tcgcaaagcc ggctacgaga acgcgttcag cctcaagggc gggctcgccg
     4021 gctggcgcgc cgacaatctg ccgctggtga agtaggatgg ccgacgaacg tgtcgtcgtg
     4081 tactcgcaac cgttttgcgg ctactgcagc gcagcgaagc ggctactgac cgcgaaaggc
     4141 gcggcgttca cggagatcga cgtcatgttc gaagccgggc gccgcgatga gatgatcgag
     4201 cgcagcggcg gccgccgaac ggttccgcag atcttcatcg atggccgcca catcggcggc
     4261 tacgacgact tgagcgctct cgacaaggcc ggcgagctcg acggtttgct cagctcgaca
     4321 gcaccctagt ttcattcacc acacacaacg actggacgaa cgatggctca agaacaaccg
     4381 acccctgctc cacagaaaca gctgctcctg caaaaaattt acgtgaagga cttgtcgttc
     4441 gagtcgccga aggcgccgat ggtcttcacg actaatgtcc aaccgcagac gcagttgaac
     4501 gtgcgctcga acgcacagca aatcgcgcag gacacgcaag aagtgacgct gaccatcacc
     4561 gtggaagcca aggacaacaa cacgacgttg ttcctcgcgg aggtcgcgca gtccggcatc
     4621 ttcgtgatcc agggctacac ggcggaagag cagtcgatca tcgtcggcag ct
//
LOCUS       DATLEE010000181         2765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3925488, whole genome shotgun sequence.
ACCESSION   DATLEE010000181 DATLEE010000000
VERSION     DATLEE010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3925488"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VM692_03885"
     CDS             <1..229
                     /locus_tag="VM692_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HUQ51336.1"
                     /translation="FPGTVVPPGVEGSVLLASAPVGRNMGFMRLGRGHQALLPPRAAY
                     RFRATDGVGVILLQTILGPHSVQKWSAICRT"
     gene            226..1245
                     /locus_tag="VM692_03890"
     CDS             226..1245
                     /locus_tag="VM692_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HUQ51337.1"
                     /translation="MSEQVQASAPNEAGYRSFSLGSFTFSRDEYFAHVEWLSKGMKLS
                     HTMSADVFLRALMRDVAWGFFYGTVNFDDVFGTRNRYGQVEFFAGCYHPTYKSQGLDH
                     LETFESGLAMQTCKAILHDWTNTGYDPFAAPMETGLAWIGKKVGHNTAAIDRARDVCR
                     RMPGLPGDLPLRTDRNRYPVNRAFLDVPQDEPEIHAAPGFEQEVHAFNLFAYLSRSDV
                     TWNPSVSSVCKQSLFCPTTEEYILPVKHANDRVEWFLQLSDQIEWQIEDGITGLPRAK
                     IVMRPGDVAAMPADIRHQGFARKRAMLLVWENADPELPRLHAQGAVPRSPAEEFWPDS
                     GSSKP"
     gene            1356..>2765
                     /locus_tag="VM692_03895"
     CDS             1356..>2765
                     /locus_tag="VM692_03895"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HUQ51338.1"
                     /translation="MIGHSKRLLASAIAAALVPSAAVLAQSEGLEEILVTAQRREQSL
                     QDVPISVTAVTGEAIEQGGFSDVEDLSIFVPNLFMRDAFTGQSLIVRGVGTSTGNEAF
                     EQAVAAFSDGVYYGRDNLSQGAVFDLERVEVVRGPQPTFAGQSATAGAINVITRKPGD
                     TWEGGVNMAYGSDEEATFDFAAGGPLSDNFGIRFAGRYYDLDDAGYTSIIGNIPQGVK
                     ENKAARITATWDPTDNLDFTFKYEHQDVWQRGTAGEYTVCETRPSFSTANTTIAPGLP
                     APCALDAVVNGSNLNVLDGKRGTGGSLDARRTVDALNAASGAAPGSANYWGNAATIGA
                     IAYDLNQVAEYNYPEDRDFQADIMLGAMNLQLGDLTLSTNTSFIEYDKEDWLDPDDSS
                     FAIFNDHRLEDFDQFAQEIRLTSPLDREVSWMVGAYYQEHTLHSRIDVYLPRLLGTAA
                     YQPAGARAVGFGGLLTEDSE"
BASE COUNT          493 a          919 c          884 g          469 t
ORIGIN      
        1 cttccccggc accgtggtgc cgcccggcgt cgagggctcg gtgctgctcg cgagcgcgcc
       61 ggtcggccgc aacatgggat tcatgcggct cggccgcggc catcaggcgt tgctgccgcc
      121 gcgcgccgcc tatcgcttcc gcgcgaccga cggcgtcggc gtgatcctgc tgcaaacgat
      181 cttgggccca cattccgtgc agaaatggtc ggcgatatgc cgcacatgag cgagcaggtc
      241 caagcgtcgg cgccgaacga agccggctat cgcagcttca gcctgggctc gttcacgttc
      301 tcgcgagacg aatacttcgc gcacgtcgag tggctctcga agggcatgaa gctgtcgcac
      361 acgatgtcgg ccgacgtgtt cctgcgcgcg ctgatgcgcg acgtcgcttg gggcttcttc
      421 tacggcacgg tcaacttcga cgacgtgttc ggcacgcgca atcgctacgg gcaagtcgag
      481 tttttcgcgg gctgctatca cccgacttac aagtcgcaag ggctcgatca cctcgagacg
      541 ttcgagagcg gccttgcgat gcagacttgc aaggcaatcc tgcacgactg gaccaacacg
      601 ggctacgacc cgttcgcggc gccgatggag acggggctcg cctggatcgg caagaaggtc
      661 ggccacaaca cggcagcgat cgatcgcgcg cgcgatgttt gccgacgcat gccgggcctg
      721 cccggcgatc tgccgctgcg gaccgaccgc aaccgttacc ccgtcaatcg cgcgtttctc
      781 gacgtgccgc aggacgagcc cgagatccat gccgcgcccg gcttcgagca agaggtgcac
      841 gcgttcaact tgttcgccta cctgtcgcgc tccgacgtga cgtggaaccc gtccgtgagc
      901 tccgtctgca agcagagcct gttctgcccg acgaccgagg aatacatcct gccggtcaag
      961 cacgccaacg atcgcgtcga gtggttcctg caattgagcg accagatcga gtggcagatc
     1021 gaggacggca tcacgggttt gccgcgcgcg aagatcgtca tgcggcccgg cgacgtcgcc
     1081 gcgatgccgg ccgacatccg ccaccaaggc ttcgcccgca agcgcgccat gttgctcgtg
     1141 tgggaaaacg ccgatcccga gctgccgcgc ctgcatgcgc aaggcgccgt gccgcgcagc
     1201 ccagccgagg agttctggcc cgactcgggc tcgagcaaac cctgacgacg cgtttgttgc
     1261 cttcttgcca atcgctgcgc gtcgtcttgt cgtcgggacg accggtgccg cgtattaatc
     1321 cctagctgga gtcaattttc ccggggggat tcgagatgat tggccattcc aagcggttgc
     1381 ttgcgtccgc cattgccgcg gctttggtgc cgagcgccgc tgttctcgcg caaagcgaag
     1441 gtctcgagga gattctcgtc acggcgcaac gccgcgagca aagcctgcaa gacgtaccga
     1501 tctcggtaac ggccgtcacc ggcgaagcaa tcgagcaggg cggcttctcg gacgtcgagg
     1561 acctgtcgat cttcgtcccg aacctgttca tgcgcgacgc gttcacgggg caatcgctga
     1621 tcgtgcgcgg cgtcggcacg tcgacgggca acgaagcgtt cgagcaggcg gtcgccgcat
     1681 tctcggacgg cgtctactac ggccgcgaca atctcagcca aggcgcggtg ttcgacttgg
     1741 agcgcgtcga ggtcgtgcgc gggccgcagc cgacgttcgc gggccagagc gcgacagcgg
     1801 gcgcgatcaa cgtcatcacg cgcaagcctg gcgacacctg ggaaggtggc gtgaacatgg
     1861 cctacggcag cgacgaggaa gccacgttcg acttcgccgc cggtgggccg ctgtccgaca
     1921 acttcggcat tcgcttcgca ggccgctatt acgatctcga cgacgccggt tacacgtcga
     1981 tcatcggcaa cattccgcaa ggcgtgaaag agaacaaagc cgcgcgcatc acggccacgt
     2041 gggatccgac cgacaacctc gattttacgt tcaagtacga gcaccaagac gtctggcagc
     2101 gcggcacggc cggcgaatac acggtctgcg agacgcggcc gagcttcagc acggccaaca
     2161 cgacgattgc gccgggattg ccggcgccgt gtgcgctcga cgccgtcgtc aacggctcta
     2221 acttgaacgt gctcgacggc aaacgcggca cgggcggctc gctcgacgct cgccgcaccg
     2281 tcgacgcgct gaacgccgcg agcggcgctg cgcccggcag tgccaactac tggggcaatg
     2341 ccgcaacgat cggtgcgatc gcctacgact tgaaccaagt cgccgagtac aactatcccg
     2401 aggaccgcga cttccaagcc gacatcatgc tgggagccat gaacctgcag ctcggcgact
     2461 tgacgctttc gacgaacacg tcgttcatcg agtacgacaa agaggactgg ctcgatcccg
     2521 acgacagctc gttcgcaatc ttcaacgacc accggctcga ggacttcgat cagttcgcgc
     2581 aagagattcg gctgacgtcg cctctcgacc gcgaggtctc gtggatggtc ggtgcgtact
     2641 atcaagagca cacgctgcac agccgcatcg acgtctactt gccgcgcctg ctcggcacgg
     2701 cggcctatca gcccgcgggg gcgcgcgccg tcggcttcgg cggcttgctg acggaggact
     2761 cggag
//
LOCUS       DATLEE010000182         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4666827, whole genome shotgun sequence.
ACCESSION   DATLEE010000182 DATLEE010000000
VERSION     DATLEE010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4666827"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2082)
                     /locus_tag="VM692_03900"
     CDS             complement(<1..2082)
                     /locus_tag="VM692_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HUQ51339.1"
                     /translation="MDAVYPAGPTTVPADLTEPTSKYRNRAWLATGGLIAFTLLYFAL
                     SGWFAWTAWRLFSAGLNGQIDTLWGWAAAFGATFLAVFMIKALFFVQHRYAIDAVEIK
                     RDEQPQLFEFLQRLADDAGAPRAHRVFVSGRVNAAVFYDLSLLNLIVPSKKNLEIGLA
                     LVNALTLGELKAVLAHEFGHFAQRSMAVGRWVYIAQQIAGQIISRRDALDGFLSGLSR
                     FDVRIAWVGWVLSLIVWSIRSLTETAFRVVLIAQRALSREMEFQADLVAVSLTGSDAL
                     IHALHKIGFAEYAWDRAVGFAAGEAQAGRRVQDVFALQTFVTQRMRDVLGDPAYGAVA
                     EVPRSKPETHRVFKQQLAQAPRMWSTHPSNNEREENAKRTYVAASIDDRTAWDVFANA
                     TALRERLSAELFRTPEQVKTVPLEESQKRLHEQYQRTYLDPQYRGAYLGRSIVRHATR
                     AEELYDAAAGAAHLSALYPGPLADELALLRDIELERGALHALRDGFMEAPGGVIQHRG
                     KELKRRDLPKAIEALEAELAAVQARVRAHDRVCRGAHLALAKSVGAGWSDYLSGLLAI
                     VHYTDHTEADLLDAHGAFRNVLSIVTADGRVSNRELKRLLASCQQLWTPLQRIYEQAA
                     EVTLDKCVRERLAIESWKGAIGELKLVRPGTENIGDWLGAVDSWVGHTAASLSALRLA
                     ALDELLLAERFL"
BASE COUNT          359 a          722 c          702 g          346 t
ORIGIN      
        1 gaggaatcgt tcggccagca acagctcgtc gagtgcggcc aagcgcaggg ccgagagcga
       61 cgctgccgtg tgcccaaccc agctgtcgac ggcgccgagc cagtcgccaa tgttctccgt
      121 gccgggtcgc acgagcttga gctcgccgat cgcacccttc cagctctcga tcgcgagccg
      181 ctcgcgcacg cacttgtcga gcgtgacctc cgcggcttgc tcatagatgc gttgcagcgg
      241 cgtccaaagt tgctggcacg aggcgagcaa gcgcttcaac tcgcggttgc tgacgcggcc
      301 gtccgccgtc acgatcgaga gcacgttgcg aaacgcgccg tgcgcgtcga gcaagtcggc
      361 ttccgtgtga tcggtgtagt gcacgatcgc gagcagtccg ctcaaatagt cggaccagcc
      421 ggcgccgaca ctcttcgcga gcgcgagatg cgcgccccgg cacacgcgat cgtgggcgcg
      481 cacccgcgct tgcacggccg cgagctcggc ctcgagcgct tcgatcgcct tcggcaagtc
      541 gcggcgcttc agttctttgc cgcggtgctg gatgacgccg cccggcgctt ccatgaaccc
      601 gtcgcgcaac gcgtgcagcg cgccgcgctc caattcgatg tcgcgcaata gcgcgagctc
      661 gtcagcgagc ggccccgggt acagcgcgct caagtgagcc gcgccggccg cggcgtcata
      721 cagctcttcg gcgcgggtcg cgtggcgcac gatcgaccgg cctagatacg cgccgcggta
      781 ctgcgggtcg agatacgtgc gctgatactg ctcgtgcagt cgcttctgcg actcttcgag
      841 cggcaccgtc ttcacttgct ccggcgtccg gaacagctcc gcagagaggc gctcgcgcaa
      901 cgcggtggcg ttcgcgaaga cgtcccaggc ggtgcggtcg tcgatcgacg ccgcgacgta
      961 ggtgcgcttc gcgttctcct cgcgctcgtt gttcgacgga tgcgtcgacc acatgcgcgg
     1021 cgcttgcgcg agctgctgct tgaagacgcg atgcgtttcg ggtttcgagc gcggcacctc
     1081 ggcgacggcg ccgtacgcgg gatcccccag cacgtcgcgc atgcgctgcg tgacgaaagt
     1141 ctgcaaggca aacacgtcct gcacgcgtcg gccggcttgc gcttcgccgg ccgcgaagcc
     1201 gacggcgcgg tcccacgcat actcggcgaa gccgatcttg tgcagtgcgt ggatcagcgc
     1261 gtcgcttccc gtcaacgaca cggcaacgag gtcggcctgg aattccatct cgcgcgacaa
     1321 agcgcgctgc gcgatcagca cgacgcgaaa ggcggtctcc gtcagcgagc ggatcgacca
     1381 gacgattagc gacagcaccc agccgaccca cgcaatgcgc acgtcgaagc gcgagagccc
     1441 gctcaagaag ccgtcgagcg cgtcacgtcg cgagatgatc tgccccgcga tttgctgcgc
     1501 gatgtagacc cagcgtccga cggccatcga gcgctgcgcg aaatggccga actcgtgcgc
     1561 gaggacggct ttcagctcgc cgagcgtcag tgcgttgacg agcgccaacc cgatctcgag
     1621 gttcttcttc gacggcacta tgaggttcag cagcgacagg tcgtagaaga ctgcggcatt
     1681 gacgcgcccc gacacgaaca cacggtgcgc gcgcggcgcg ccggcatcgt cggccaagcg
     1741 ttgcaagaac tcgaatagct gcggctgctc gtcgcgcttg atctcgacgg cgtcgattgc
     1801 gtagcggtgc tgcacgaaga acaacgcctt gatcatgaac acggcaagaa acgtcgcgcc
     1861 gaacgccgcg gcccagcccc agagcgtgtc gatctggccg ttgagcccgg cgctgaacaa
     1921 ccgccacgcg gtccacgcga accagcccga cagcgcgaag tagagcagcg tgaatgcgat
     1981 cagcccgccc gtcgcgagcc acgcgcggtt gcggtatttg gaggtcggct cggtgaggtc
     2041 tgccggtacg gtcgtgggtc ctgccggata gacagcatcc atcttgttct cccgtcgtgc
     2101 cggctcgaaa ggtaaccttc tcaagggcg
//
LOCUS       DATLEE010000183         3506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2437614, whole genome shotgun sequence.
ACCESSION   DATLEE010000183 DATLEE010000000
VERSION     DATLEE010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437614"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VM692_03905"
     CDS             complement(<1..488)
                     /locus_tag="VM692_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUQ51340.1"
                     /translation="MNAATNEIAAVGAHLNEVYPMLPFTPVRGMGVYLENAAGRRVLD
                     LYGGHAVAALGYGHRDLSETLARQSRELVFQTNALPLKIRDEAADALAAFGPPSLGRV
                     FFVNSGAEANENALRLAFKVTGRTKVVAMEHSFHGRTAAAAAVTHGAAKTWYGFPRAP
                     FDV"
     gene            complement(489..1553)
                     /gene="argC"
                     /locus_tag="VM692_03910"
     CDS             complement(489..1553)
                     /gene="argC"
                     /locus_tag="VM692_03910"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HUQ51341.1"
                     /translation="MSNDKTIPTIVLGGSGYVAGEFLRLIAGHSQLTLGGVVSTSQAG
                     EPVAKTFPHLAPTYPDTLFVSLEAVIERLNTAPRWLLLSAAPHGASAGLVDRMLKAAA
                     SAGVELVVVDASADFRFADAAAYAAVYGHPHETPALLKDFTCAVPEQLAQIETPHAAH
                     PGCFATTMLLPIVPLAALGLADEFFVSAVTGSTGAGRTPRDTTHHPLRQSNLFAYQAL
                     KHRHDPEVRALIKAATGHDVRLSFVPHSGPFARGIHATIFLHAKERLTQAQVNEALRE
                     YYRGATFVRVDDEPPKMKDIAATNYAALHAVVQGDTIVVCSVTDNLLKGAAGGSLQWA
                     NRLLGFPEATGLTAPAAGWL"
     gene            complement(1550..2623)
                     /locus_tag="VM692_03915"
     CDS             complement(1550..2623)
                     /locus_tag="VM692_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51342.1"
                     /translation="MRSVLVDKIASVAVQADLGQEIRVSPDIPCEEGVLIAVEVLNNK
                     SRYNTIELTSGRMAEVKKGDVDVGALGQRKALVGYSGQLPTELKPGDIVQLLNLGGVI
                     GICDSINPSHGEPFNCKVLGCVLDFPYLGQRVGVPARVSTEPLKLDVPIDTHGIPVVA
                     IAGTCMNSGKTAAACAIVSRFRHNGLVVDAMKSTGVALRRDILAMEDSGARHVAMFSD
                     FGICATTSKNAPALTRSLLNELSDEKPDAIVFELGDGLLGAYGVEAILRDKDIVGKIT
                     AMVLCANDPVGAWGGAKLLREEFGIEPTVISGPATDNQVGIDIIKDRSNVAALNAMTS
                     SAELGDLLQASIGLGHRTAKAGA"
     gene            complement(2625..>3506)
                     /locus_tag="VM692_03920"
     CDS             complement(2625..>3506)
                     /locus_tag="VM692_03920"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HUQ51343.1"
                     /translation="AKDLGSDTVAHGCTAAGNDQVRFEIVLKAVAPQLTILAPVRDHA
                     FQRPAQVKFLEERNMPVPTRGSAYSTNRGLWGVTIGGTETLDSKGSIPESAWVLSAGA
                     LDKPQPFSEIELEFVHGVPVSLDGKRLEPVALLEKVEELGARYGIGRGIHLGDTIIGT
                     KGRVAFEAPAAEILISAHRELEKLTLLGSQAVVKEPVSQVYGQLIHEGKQLDPACRDI
                     EALLSSSQARVTGKVRVQLRPGSLFVLGVTSPHSLMAVSRGKYGEAAGEWTPADAYGF
                     SKIMALPGMFHTRAGGQ"
BASE COUNT          570 a         1186 c         1164 g          586 t
ORIGIN      
        1 acgtcgaacg gcgcgcgcgg aaagccgtac caagtcttcg cggcgccgtg cgtcacggcc
       61 gccgccgcgg ccgttcgacc gtggaagctg tgctccatcg cgacgacttt agtgcgcccc
      121 gtgaccttga acgcgagccg cagcgcattc tcgttcgcct cggcgccgct gttgacgaag
      181 aagactcgcc ccaagctggg cggcccgaac gcggcgagcg cgtccgcggc ttcgtcgcga
      241 atcttcagcg gcaacgcatt ggtttggaaa acgagctcgc ggctctgccg cgccagcgtc
      301 tcgctcaagt cgcgatgccc atagccgagc gccgcgacgg cgtggccgcc atagaggtcg
      361 agtacgcggc ggcccgccgc attctcgaga tacacgccca tgccgcgcac gggagtgaac
      421 ggcagcatcg gatagacctc gtttaagtgc gcgccgaccg cagcgatttc gttggttgcc
      481 gcgttcatct agagccaccc tgccgcaggt gcggtcaaac ccgtcgcttc cggaaaaccc
      541 aagagcctat tggcccattg caggctgccg cctgccgcgc ccttgagcaa attgtcggtc
      601 acggagcaca cgacgatcgt atcgccctgc acgacggcat gcagcgcggc gtagttcgtc
      661 gcggcgatgt ccttcatctt cggcggctcg tcgtcgacgc gcacgaacgt ggcgccgcga
      721 tagtattcgc gcagcgcttc attgacttgc gcttgcgtga gccgctcctt ggcgtgcagg
      781 aaaatcgtcg cgtgaatgcc gcgagcgaac gggcccgagt gcggcacgaa gctcaagcgc
      841 acgtcgtggc ctgtcgccgc cttgatcagc gcgcgcacct cgggatcatg gcgatgcttc
      901 aacgcttgat acgcgaacag gttgctctgc ctcaaggggt ggtgcgtcgt atcgcgcgga
      961 gtgcggcccg cgcccgtgct gcccgtgacc gcgctgacga agaactcgtc ggcgagtccg
     1021 agggcggcaa gcggcacgat cggtagcagc atcgtcgtag cgaagcagcc gggatgcgcc
     1081 gcgtgtggcg tctcgatctg cgcgagctgc tccggtacgg cgcaggtgaa gtccttcagc
     1141 aacgccggcg tctcgtgcgg atggccgtag acggccgcat aggcggccgc gtcggcgaaa
     1201 cgaaagtcgg ccgacgcatc gaccacgacg agctcgacgc cggcactcgc ggcggccttc
     1261 agcatgcggt cgacgagtcc cgccgacgcg ccgtgcggcg cggctgacaa cagcagccag
     1321 cgcggggccg tgttcaagcg ctcgatgacg gcctctagcg agacgaacaa cgtgtcggga
     1381 tacgtcggcg cgagatgcgg aaacgtcttc gcgacgggct cgccggcctg gctcgtcgag
     1441 acgacgccgc cgagcgtgag ctgcgaatgg cccgcgatga ggcgtaggaa ttcgccggcg
     1501 acgtagcccg agccgccgag cacgatcgtg ggaatcgtct tgtcgttgct catgcgccgg
     1561 ccttggccgt ccggtggccg agcccgatgc tcgcttgcaa tagatcgccg agctccgccg
     1621 agctcgtcat cgcgttcaac gccgcgacgt tcgagcggtc tttgatgatg tcgatgccga
     1681 cctgattgtc cgtggcagga cccgagatca cggtcggctc gatgccgaac tcctcgcgca
     1741 acagtttcgc gccgccccaa gcgccgacgg ggtcattggc gcagagaacc atcgccgtga
     1801 tcttgccgac gatgtccttg tcgcgcagga tcgcctcgac gccgtacgcg ccgagcagac
     1861 cgtcgccgag ctcgaaaacg atggcgtctg gtttctcgtc cgagagctcg ttcagcagcg
     1921 agcgcgtcag cgctggcgca ttcttcgacg tcgtcgcgca gatgccgaag tcggagaaca
     1981 tggcgacgtg gcgcgcgccc gagtcttcca tcgcgaggat gtcgcggcgc agcgcgacgc
     2041 ctgtcgactt catcgcgtcg acgacgagcc cgttgtggcg gaagcggctc acgatcgcgc
     2101 aggcagccgc ggtcttgccg gaattcatgc aggtgccggc aatcgcgacg acggggatac
     2161 cgtgcgtgtc gatcggcacg tcgagcttca gcggctccgt cgaaacgcgc gccggcacgc
     2221 cgacgcgctg gccgaggtag ggaaagtcca gcacgcagcc gagcaccttg cagttgaacg
     2281 gctcgccgtg cgacgggttg atcgagtcgc agatgccgat gacaccgccc aagttcaaca
     2341 gctgcacgat gtcgccgggc ttgagctccg tcggcaactg acccgagtac ccgaccagcg
     2401 ccttgcgctg gccgagcgcg ccgacgtcga cgtcgccctt tttcacctcg gccatgcggc
     2461 cgctcgtgag ctcgatcgtg ttgtagcgcg atttgttgtt gagcacctcg acggcgatca
     2521 gcacaccttc ctcgcacgga atgtcggggc tcacgcgaat ctcttgcccg agatccgctt
     2581 gcacggcgac cgacgcgatt ttgtcgacga gaacggagcg catctcactg ccctccggcc
     2641 cgggtgtgga acatcccggg caaagccatg attttcgaga acccataggc atcggccggc
     2701 gtccattcgc cggcggcttc gccgtacttg ccgcggctca cggccatcag cgagtgcggc
     2761 gacgtgacgc cgagcacgaa taggctgccg ggccgcagct gcacacgaac tttgcccgtc
     2821 acgcgggcct gcgacgacga gagcagtgcc tcgatgtcgc ggcacgcggg atcgagctgc
     2881 ttgccttcgt gaatcagctg gccgtagacc tggctcacgg gctccttcac gacggcttgc
     2941 gagccgagca gagtcagctt ctcgagctcg cggtgcgcgc tgatcaagat ctcagcggcc
     3001 ggcgcctcga acgcgacgcg gcctttcgtg ccgatgatcg tgtcgcccaa gtgaatgccg
     3061 cggccgatgc cgtagcgcgc gccgagctcc tcgaccttct cgagcagcgc gacgggctcg
     3121 aggcgcttgc catcgaggct gacgggcacg ccgtgcacga actcgagctc gatctcgctg
     3181 aacggctgcg gcttgtcgag cgctcccgcg gacagcaccc acgccgactc cgggatactg
     3241 cccttcgagt cgagcgtctc ggtgccgccg atcgtgacgc cccagaggcc gcgattcgtc
     3301 gaatacgccg agccgcgcgt cgggaccggc atgttgcgct cctcgaggaa cttcacttgc
     3361 gccgggcgct gaaacgcgtg atcccgcacc ggcgccaaga tcgtcagctg cggcgcgacg
     3421 gccttcagca cgatctcgaa ccgcacctgg tcgttgcccg ccgccgtgca gccgtgcgcg
     3481 acggtgtccg agccgaggtc cttcgc
//
LOCUS       DATLEE010000184         3121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3927514, whole genome shotgun sequence.
ACCESSION   DATLEE010000184 DATLEE010000000
VERSION     DATLEE010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3927514"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VM692_03925"
     CDS             complement(<1..313)
                     /locus_tag="VM692_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018872233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HUQ51344.1"
                     /translation="MRPIGIFGGTFDPIHYGHLRTALELSQRLDLAAVHFVPVANPPH
                     RSTPLNDGTVRLRMVQAAIRGEPGFVADDRELKRSGLSYTVDTLTSFRSELGAERSLC
                     LL"
     gene            complement(317..1345)
                     /gene="holA"
                     /locus_tag="VM692_03930"
     CDS             complement(317..1345)
                     /gene="holA"
                     /locus_tag="VM692_03930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HUQ51345.1"
                     /translation="MKVPFRQLESHLSRGLAKVYLVAGDEPLLVDEALEQVRAAAMRT
                     GFTSRELHTAERTFRWVELLAGADNLSLFATRKIVEIRLATPKPGDVGSSTIATLSER
                     EDPDTLLIIAVSEKLDSATQKTAWVKAIEKHGVIVEIWPIERGELPRWIQQRATMQRL
                     KLTSAAAQSLAERVEGNLLAADQEIKRLALTAAGREIDEAEVLEFVANNSRFDVFALA
                     DAVLAGETGRTFKILSGLRAEGVHPVQISWVLNRDISLLARLEYAVRHGDNIDGALLR
                     SGVWRRRQPLVRQALAKFKAPRLKTLLAEAARVDAALKGVFPAEPWTTLTDLLVALLR
                     PPEGARAR"
     gene            complement(1342..1602)
                     /locus_tag="VM692_03935"
     CDS             complement(1342..1602)
                     /locus_tag="VM692_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HUQ51346.1"
                     /translation="MDRKLLDIICCPVTRSSLELLSEQELATLNQLIGVRRIKNREDS
                     VVDAPLTEALVTRSGKLIYPVRDGIPVLLEDQAMPLQQLEGS"
     gene            complement(1602..1844)
                     /gene="yidD"
                     /locus_tag="VM692_03940"
     CDS             complement(1602..1844)
                     /gene="yidD"
                     /locus_tag="VM692_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HUQ51347.1"
                     /translation="MVRALLLGLIRLYQLTLSPWLGTQCRYEPTCSKYAAEALERFGV
                     IRGIWLAARRLGRCHPWGRSGYDPVPAADGPPARTP"
     gene            complement(1856..>3121)
                     /gene="cysS"
                     /locus_tag="VM692_03945"
     CDS             complement(1856..>3121)
                     /gene="cysS"
                     /locus_tag="VM692_03945"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HUQ51348.1"
                     /translation="VTDVDDKINDAVKKEGVPIGVLTARYLAAYHEDLRALGVAPPDV
                     EPRVTDNISSIVGFIERLIAAGHAYEAEGHVLFAVPSFAQYGQLSGRDPEDMLAGARI
                     EVAPYKRDPGDFVLWKPSEPELVGWPSPWGRGRPGWHIECSAMAAEHLGETIDIHGGG
                     VDLLFPHHENELAQSTCGHGGKTFARYWLHNGLIQFDGGKMSKSLGNVRLARELLAEH
                     GGEAVRLSLLTAHYRQPLDWTDQLVVESKQKLDRLYGALRDAGVSGDLTRAPAALPPP
                     GVLAALEDDINTPEAIAELFGVVRATNKATEPAEKRALAESLRAGAWLLGLLTQEPAE
                     WFRGAGLPPGKSMTEAVHRAKEAPQASAQGTPTALLEHAEIDSLVAQRDALKRQRNFK
                     EADLIRDRLAAQGIVIEDTPQGARWRRGR"
BASE COUNT          485 a         1053 c         1052 g          531 t
ORIGIN      
        1 gcaacaaaca cagcgaccgc tcggcgccga gctcgctgcg gaacgacgtc agcgtatcca
       61 ccgtgtatga caagcccgag cgctttagtt cgcggtcgtc ggcgacaaag ccgggctccc
      121 cgcggatcgc cgcttgaacc atgcgcaggc gcacggtgcc gtcgttgagc ggcgtcgagc
      181 ggtgcggcgg attggccacg ggcacgaaat gcacggcggc caggtcgagc cgttggctta
      241 gctcgagtgc cgtgcgcaga tggccgtagt ggatggggtc gaacgtgccg ccgaaaatgc
      301 cgattggtcg catcggtcag cgggccctcg cgccttcggg cgggcgcaac agcgcgacca
      361 agagatccgt cagcgtcgtc cacggctcgg ccggaaacac gcccttcagc gccgcatcga
      421 cacgcgccgc ctcggccagc agcgtcttca accgcggcgc tttgaatttc gcgagcgctt
      481 ggcgcacgag cggctggcgc cgccgccaga cgccgctgcg tagcaacgcg ccgtcgatgt
      541 tgtcgccgtg gcgaacggcg tactcgaggc gcgcgagcaa gctgatgtcg cggttcagca
      601 cccagctaat ctgcacgggg tgcacgccct cggcgcgcag gcccgacaga attttgaacg
      661 tgcgacccgt ctcgccggcc agcacggcgt cggccagcgc aaacacgtcg aagcggctgt
      721 tgttcgcgac gaactcgagc acctcggctt catcgatctc gcggccggca gccgtcagcg
      781 cgaggcgttt gatttcctgg tccgcggcca ggagattgcc ttcgacgcgc tccgcaagcg
      841 attgcgcagc ggcgctcgtc agcttcaagc gctgcatcgt ggcccgctgc tggatccagc
      901 gcggcagctc accgcgctcg atcggccaaa tctcgacgat gacgccgtgc ttctcgatcg
      961 ctttgaccca cgcggtcttc tgcgtcgcgg aatcgagttt ctcgctgacc gcgatgatca
     1021 gcagcgtgtc ggggtcctcg cgctcgctca aggtcgcgat cgtgctcgag cctacgtcgc
     1081 cgggcttcgg cgtcgcgagc ctgatctcga cgatcttgcg cgtcgcgaac agcgacaagt
     1141 tgtcggcgcc cgcgagcaat tcgacccaac ggaaagtgcg ctcggccgtg tgcagctcgc
     1201 ggctcgtgaa gccggtgcgc atcgcggcgg cgcgcacttg ctcgagcgcc tcgtcgacga
     1261 gcagcggctc gtcacctgcg acgagataaa ccttggccag accgcggctg agatggcttt
     1321 cgagctgacg aaacgggact ttcacgaacc ctcgagttgc tggagcggca tggcctggtc
     1381 ctcgagcagg accggaatgc cgtcgcgcac cggatagatg agcttgccgc tgcgcgtcac
     1441 gagcgcttcg gtcagcggcg cgtcgactac cgagtcctcg cggttcttga tgcgtctcac
     1501 gccgatgagc tggttcagcg ttgccagctc ctgctcggac agcagctcga gcgacgagcg
     1561 tgtgacgggg cagcagatga tgtcgagcaa cttgcggtcc atcacggcgt ccttgccggc
     1621 ggtccgtcgg cggccggtac cggatcgtac cccgagcggc cccagggatg gcagcggccg
     1681 aggcgtcttg ccgcgagcca aatgccgcgg ataacgccga aacgctcgag ggcttcggcg
     1741 gcgtacttcg agcaggtggg ctcgtagcgg cactgcgtgc cgagccaagg ggacagcgtc
     1801 aactgataga gacggatcag gccgagcagc agtgctcgta ccatgttgcc gaggctcaac
     1861 gccctcgtct ccatcgggca ccttgcggcg tgtcttcgat cacgatccct tgtgcggcca
     1921 gtcgatcacg tatgaggtcg gcttccttga agttgcgctg ccgtttcaaa gcgtcgcgct
     1981 gcgcgaccag gctgtcaatc tcggcgtgct ccaacagcgc ggtgggcgtg ccctgggcag
     2041 aagcttgcgg cgcctccttt gcgcggtgca ccgcttccgt catggacttt ccaggaggca
     2101 gtccggctcc tctaaaccac tccgccggct cctgcgtgag taagccgagc agccacgcgc
     2161 cggcgcgcag cgattcggcg agcgcgcgct tttccgcggg ctccgtcgcc ttgttcgtcg
     2221 cgcgcacgac accgaacagc tcggcgatgg cttcgggcgt gttgatgtcg tcctcgagcg
     2281 ccgcgagcac gccgggcggc ggcagcgccg ccggcgcgcg cgtgaggtcg ccgctgacgc
     2341 cggcgtcgcg cagcgcgccg tagaggcggt cgagcttctg cttcgattcg acgacgagct
     2401 ggtccgtcca atcgagcggc tggcgatagt gcgccgtcag caacgagagc cgaacggcct
     2461 cgccgccgtg ctcggccagc agctcgcgcg cgagccgcac gttgccgagc gacttcgaca
     2521 tcttgccgcc gtcgaattga atgagcccgt tgtgcagcca ataacgcgcg aacgtcttgc
     2581 cgccgtgccc gcaggtgctc tgggcgagct cgttctcgtg gtgcggaaac agtaggtcca
     2641 cgccgccgcc gtgaatgtcg atcgtctcgc cgagatgctc cgcggccatc gcggagcact
     2701 cgatgtgcca acctgggcgg ccgcggcccc acgggctcgg ccaaccgacg agctcgggct
     2761 ccgacggttt ccacagcacg aagtcgccgg ggtcgcgctt gtacggcgca acttcgatgc
     2821 gtgcgccggc cagcatgtcc tcggggtcgc ggcccgagag ctgcccgtac tgagcgaagc
     2881 tcggcaccgc gaacagcacg tggccttcgg cttcgtaggc atgcccggcg gcaatcagcc
     2941 gctcgatgaa gccgacgatg ctcgaaatgt tgtccgtgac gcgcggctcg acgtcgggcg
     3001 gcgcgacacc gagcgcccgc aagtcttcgt gataagccgc gagatagcgc gcggtcagca
     3061 cgccgatcgg cacgccctct ttcttcacgg cgtcgttgat cttgtcgtcg acgtccgtga
     3121 c
//
LOCUS       DATLEE010000185         4899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7553470, whole genome shotgun sequence.
ACCESSION   DATLEE010000185 DATLEE010000000
VERSION     DATLEE010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7553470"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="VM692_03950"
     CDS             <1..1470
                     /locus_tag="VM692_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51349.1"
                     /translation="VARGADHVVVEHGSWVETGESFFAEGAWNGPFGEPSLGDADIVL
                     GSGGTLVAENVAFTPTTHTMERLYSHESPTAVLVANSLPFLLEACQAKLDLRNWSYER
                     QLMTFLRGYKKAVKRLELAGGATVQLHYHDTLLIGPDLRQRVKHPPAPPRFACYGAYV
                     DYLTRSLGTLHRNASSPQRAVAYTPLSTISSGYDSPACAVLAKRLGCRRTVTFSHARE
                     DFNERPLKTLDDSGEHIAKMLELDVTTFSRNAYLAADDYPEALFIATGSGGDDVVMSV
                     LRETLEGTMLFTGMLGDTLWSTHDTQSPELSEQYRFLYPAGGSLQEFRLQTGFVHVPV
                     PLLTFTRHADLRRISNSLEMRQWRVGGHYDRPIPRRLVEDEGVPRRAYAREKRAISQP
                     FWLQKSDASCMSARSLRDLEAFRRRAAARYAFGELRMRINESWQRAAANWRKRVARLA
                     RDPYHSDAYLDAGVADPLRFHWAVEKVAAAYRGSELRTL"
     gene            complement(1505..3028)
                     /locus_tag="VM692_03955"
     CDS             complement(1505..3028)
                     /locus_tag="VM692_03955"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HUQ51350.1"
                     /translation="MSAASSSRSMLEVNGLLVASVGVGFLNNAAIAWLFGLTREVDAY
                     YAAMFLPGMFMVLFIEYLGKNFLPVFAQARKHSVETASELTSTMVTVCALITAAVIGV
                     LIVASKPIFTVVLPGFTPAEIDLVTRYLWIMAPTSVLSAVSTFHAYLFQHEDKYTQIA
                     AIGALQPVINLLAVLALGPFLGAYALPIGFTVGKVVAFVRYARGAHYRYRPRLTMRRE
                     WEQRVFINSAILMGTGLLVRTRELLGNYFASQLGEGAIAALALGYKLVEPLERTTFGG
                     VRMLMYSRTARLAVEQNAGEMSRIYRLGIQASFLLVAPPLAWMCYESEFLVRLLFERG
                     AFDMSMTALVALAIIGFSPSILLAGINGVLSNAFYALDRVKIPALVMPVGTIAYLAVA
                     PFVYKPFGVLGLSLSPTPAYLVVFLLLLYFLNKRLPALHGGGVLLKAIGYTGLAVVAF
                     GVPKLLLTAVEWPGLVEATTSLLGGGALYAGVLVALRDGTFREVYAYFRRAYPGFAV"
     gene            3188..3790
                     /locus_tag="VM692_03960"
     CDS             3188..3790
                     /locus_tag="VM692_03960"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HUQ51351.1"
                     /translation="MKKDFVWLASASPRRSELLRQIGVPFRVQAADIREEQLAGEVPE
                     AYVLRLAAAKAGAVWAALAEGERRPVLAADTAVVLDGAVLGKPKDAAEAESMLERLSG
                     RVHRVLTAVSLRCSADRATLLSTSEVRFRPTTAAERVAYCATGEPFDKAGGYGIQGHA
                     AVFVEELRGSYSGVVGLPLFETAALLARGGFSFWLTARAA"
     gene            3790..>4899
                     /locus_tag="VM692_03965"
     CDS             3790..>4899
                     /locus_tag="VM692_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HUQ51352.1"
                     /translation="MKREEILINATPSEVRAALVESGVLQEVQIERTSRVGLISNIYK
                     GKVSRVLPGMQAAFVELGLERTAFLHASDIARSPHADAGASEVEPDIREFVREGQELL
                     VQVLKEPLGTKGARLTTYIAIPSRYLVLTPNGTGIGVSSRIDDEAERERLRCLVEELK
                     VAAPEAGYIIRTAAEGATREALQADLQFLHKLWASIEEAAARTSAGGLVYEDLPLPLR
                     VLRDSSSPDVTRVRVDSEATCAKLKEFAERFVPRLAPLIEVYTESRPIFDVYGIDDEI
                     NRALNRKVSLKSGGHVVFDQTEAMTTIDVNTGAYVGHRNLEETIFKTNLEAAAAIARQ
                     LRLRNLGGIIIIDFIDMAEEEHRRQVLQTLTDRLAG"
BASE COUNT          909 a         1573 c         1640 g          777 t
ORIGIN      
        1 gtcgcgcgcg gcgccgacca cgtcgtcgtc gagcatggct cttgggtaga gaccggcgag
       61 agctttttcg ccgagggtgc ctggaacggg ccgttcgggg agccgagcct cggcgacgcg
      121 gacatcgtgc tcggatccgg aggaacgctc gttgcagaaa acgtcgcgtt cacgcccacg
      181 acgcacacga tggagcggct ctacagccac gaatcgccga cggccgtgct cgtcgcgaat
      241 tcgctgccgt tcttgctcga ggcctgccaa gccaagctcg acctgcgcaa ttggtcatac
      301 gagcgccagt tgatgacctt cctacgcggc tacaaaaaag ccgtgaagcg gcttgagctc
      361 gcaggcggcg ccaccgttca gttgcactat cacgacacgc tgttgatcgg ccccgatctg
      421 cggcaacgcg tgaagcatcc gccggccccg ccgcggttcg cttgctacgg cgcgtacgtc
      481 gattacctca cgcgcagtct cgggaccttg caccgcaacg cgagctcgcc gcagcgcgcc
      541 gtcgcgtaca cgccgctgtc gaccatctct tcgggttacg actcgccggc ctgcgcggtg
      601 ctcgcgaaac ggctcggctg ccggcggacg gtaacctttt cgcacgcgag agaggacttc
      661 aacgaacggc cgttgaaaac gctcgatgac agcggtgagc acatcgcgaa gatgctcgag
      721 ctcgacgtca cgacgttttc ccgcaacgcg tacctcgcgg ccgacgatta ccccgaggcg
      781 ttgttcatcg ccaccggcag cggcggcgac gacgttgtca tgagcgtgct gcgcgaaact
      841 ctcgagggaa cgatgctgtt cacgggcatg ctcggcgaca ccttatggag cacccacgac
      901 acgcaaagcc ccgagctcag cgagcagtac cgcttcctgt atccggccgg cggcagtctc
      961 caggaatttc ggctgcaaac gggcttcgtg cacgtgccag ttcccttgct cacgtttacg
     1021 cgtcatgccg acttgcgacg catctcgaac tcgctcgaga tgcggcagtg gcgcgtaggc
     1081 ggtcactacg accgcccgat cccgaggcgg ctcgtcgagg atgaaggcgt gccgcggcgg
     1141 gcatacgcga gagagaagcg ggcgatctcg caaccgtttt ggctgcagaa gtcggacgca
     1201 agctgcatga gcgcgcgatc gctgcgcgat ctcgaagcgt ttcgacgtcg cgccgccgcg
     1261 cgttacgcgt tcggcgagct acggatgcgg atcaatgaaa gttggcagcg ggccgcggcc
     1321 aactggcgca agcgagtcgc gcgtcttgcg cgcgacccgt atcactcgga cgcgtatctc
     1381 gacgcgggag tggcggaccc gttgcgtttc cattgggccg tcgagaaggt cgccgccgct
     1441 taccgcggct cggagctgcg aactctttag aggccttggc gccgccggtg cgatacaaca
     1501 tcggtcaaac cgcgaagccg gggtaggcac gcctgaagta cgcgtagacc tcgcggaacg
     1561 tgccgtcgcg tagggcgacc agcacgccgg cgtagagcgc cccgccgccg agcaggctcg
     1621 ttgtagcttc gacgagcccc ggccactcga ccgccgtcag caaaagcttc gggacgccga
     1681 atgcgacgac ggcgagaccg gtgtagccga tcgctttgag caggacaccc ccgccgtgca
     1741 gcgccggcag gcgcttattg aggaaataca gcagcaacag gaacacgacc aagtaggcgg
     1801 gggtcggcga aagcgacagg ccgagcacgc cgaacggttt gtaaacaaac ggtgcaacgg
     1861 ccagatacgc gatcgtgccc acgggcatca cgagagccgg aattttgacg cggtccaacg
     1921 catagaaggc gttcgacagc acgccattga tgcccgcgag caagatcgac ggcgagaagc
     1981 cgatgattgc gagcgcgacg agcgccgtca tgctcatgtc gaacgcgccg cgctcgaaca
     2041 gcagccgcac gagaaactcg ctctcgtagc acatccacgc gagcggcggt gcgacgagca
     2101 gaaagctggc ctggatgccc aagcgataga ttcgcgacat ctcgcccgcg ttttgctcga
     2161 cggcgagccg cgccgtgcgg ctatacatca acattcgcac gcccccgaac gtcgtgcgct
     2221 cgagcggctc gacgagcttg tagccgagcg cgagggcggc gatcgcgcct tcgccgagct
     2281 gcgaggcgaa ataattgcca agcagctcgc gcgtccggac gagtagcccg gtgcccatga
     2341 ggatcgccga gttgatgaac acgcgttgtt cccattcccg ccgcatcgtg agacgcggcc
     2401 gatagcggta atgcgcgccg cgcgcatagc gcacgaaggc gacgactttg ccaaccgtga
     2461 acccgatcgg cagtgcatag gcgccgagga acgggccgag agctagaacc gcgagcaggt
     2521 tgatcacggg ttgcagcgcg ccgatcgccg cgatctgcgt gtatttgtcc tcgtgttgga
     2581 aaaggtaggc atgaaacgtc gatacggcgc tcaacacgct ggtcggcgcc atgatccaca
     2641 gataccgggt cacgaggtcg atctcggcag gagtgaagcc cggcagcacg accgtgaaga
     2701 tgggcttgct cgcgacgatc agcacgccga tcacggcggc ggtgatcaac gcgcagaccg
     2761 tgaccatcgt cgacgtgagc tcggatgccg tctcgacgct gtgttttcgg gcctgcgcga
     2821 agacgggcag gaagtttttg ccgagatact cgatgaacag caccatgaac atgccgggca
     2881 ggaacatcgc ggcgtagtag gcgtcgacct cgcgcgtaag gccgaatagc cacgcgatgg
     2941 cggcgttgtt cagaaacccc acgccgaccg acgccacgag cagcccattg acctcgagca
     3001 tgctgcgcga gctcgaggct gcgctcatcg cgataccgac ctaaacggca ggcgccacgc
     3061 catcgatggt tgtctctctc gtggggagga aggggcctat acgattatcg ctcgcggcct
     3121 cggtcggacg gcgcccggcc agttcaaggc tacccgtaac acgcactagc tcgtatagtg
     3181 cgttgaaatg aaaaaggatt tcgtctggct ggcgtccgcg tcgccgcggc gtagcgagct
     3241 gctgcgccag atcggcgtgc cgtttcgtgt gcaagcggca gatataagag aggagcagct
     3301 cgccggggag gttcccgagg cctacgtcct gcgtctcgcg gcggccaagg ccggcgccgt
     3361 ttgggcggcc ctcgccgaag gggagcgccg gcccgtgctt gcggccgaca ccgccgtcgt
     3421 tttagacggc gccgtgctcg gtaagccgaa ggacgccgcc gaggcggaaa gcatgctcga
     3481 gcgcctgtcc gggcgagtgc accgcgtgct gactgccgta tcgctgcgct gttccgccga
     3541 tcgagcgacg ctcctcagta cgagcgaagt gagattccgg cccacgaccg ccgcggaacg
     3601 cgtcgcgtac tgtgcgacgg gcgagccgtt cgacaaggca ggcggctacg gcatccaagg
     3661 gcacgcagcg gtgttcgtcg aggagttacg cggcagctat tcgggggtcg tcgggctgcc
     3721 gctattcgaa acagcggcgc tgctcgcgcg cggcggtttc agcttctggc tcacggcgcg
     3781 agcggcctga tgaagcgcga ggagatcctc atcaacgcaa cgccgagcga ggtgcgggcc
     3841 gccctcgtcg aaagcggtgt gctgcaagag gtgcaaatcg agcgcacgag ccgcgtcggc
     3901 ctcatcagca acatctacaa gggcaaagtc tcgcgcgtgc tgccgggcat gcaagccgcc
     3961 ttcgtcgagc tcggcctcga acgtacggcg ttcctgcacg cgtcggacat cgcgcgcagc
     4021 ccacacgcgg acgccggcgc gagcgaggtc gagcccgata tccgcgagtt cgtgcgcgag
     4081 ggccaggagt tgctcgtcca ggtgctcaag gagccgcttg gcaccaaggg cgcgcggctg
     4141 acgacgtaca tcgcgatccc ctcgcgctac ctcgtgctga cgccgaacgg tacgggcatc
     4201 ggggtgtcgt cgcgcatcga cgacgaggcg gaacgcgagc ggttgcgctg cctcgtcgag
     4261 gagctgaagg tcgcggcgcc ggaagccggc tacatcatcc gcacggcggc cgagggcgcc
     4321 acgcgcgagg ccctgcaggc ggacctacag ttcctgcaca agctctgggc ctcgatcgag
     4381 gaggctgccg cacggacgtc ggccggcggg ctcgtttacg aggatttgcc gctgccgctg
     4441 cgcgtgctgc gcgactcgtc gagccccgac gtcacgcgcg tgcgcgtcga ttccgaggcg
     4501 acgtgcgcca agctcaagga gtttgccgag cgtttcgtgc cgcggctggc gccgctgatc
     4561 gaggtctata cggaaagccg gccgatcttc gatgtctacg gcatcgacga cgagatcaac
     4621 cgtgcgctga accgtaaggt gtcgttgaag tccggcggcc acgtcgtgtt cgaccagacg
     4681 gaagccatga cgacgatcga cgtcaacacg ggcgcctacg tcggccaccg caatctcgag
     4741 gagacgatct tcaagacgaa cctcgaggcg gccgccgcga tcgcgcggca actgcggctg
     4801 cgcaatcttg gcggtatcat cattatcgac ttcatcgaca tggccgaaga ggagcaccgc
     4861 cgccaggtgc tgcagacgct cacggatcgc ctggccggc
//
LOCUS       DATLEE010000186         3296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3088078, whole genome shotgun sequence.
ACCESSION   DATLEE010000186 DATLEE010000000
VERSION     DATLEE010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3088078"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VM692_03970"
     CDS             complement(<1..673)
                     /locus_tag="VM692_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUQ51353.1"
                     /translation="MPSPHVLEPSRHTPIYGDYDVVVLGGGPAGLAAAIAAGRAGRST
                     ICIERYGFCGGAGTAAGLSTFCGLHAVVHGKHEQIVHGIADDILARLERMDGLNKPHL
                     TIQNQILAQAYDISAFKIAADELLAEANVHVLFHAFATGAVMANDKQIEAVLVETKSG
                     RFAIRGKIFIDGSGDGDLAAWAGVPYEVGDGAGNMLYPSTMFRINGVDPAKAGRAWEL
                     VPKLMD"
     gene            complement(780..1307)
                     /locus_tag="VM692_03975"
     CDS             complement(780..1307)
                     /locus_tag="VM692_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HUQ51354.1"
                     /translation="MRSTLCLGLIAAALAGAVLAQDDAGQRWPVPGVTAPVTELALEL
                     YVTIAPAVVVGQSDHGTRQFIPITGGRFSGANSLKGEVMAGGADWQLRRPDGVTEVKA
                     IYSIKTGDGAVIEVDNQGIIVPAAADGGRYVRTNVRFHAPQGPHEWLNKASFVGTITP
                     SPQGGAVIIRAFKVL"
     gene            1440..2468
                     /locus_tag="VM692_03980"
     CDS             1440..2468
                     /locus_tag="VM692_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Virginiamycin B lyase"
                     /protein_id="HUQ51355.1"
                     /translation="MRREVSIVATLLAAGCASAQQRWLKDDERLTATGEVTELVLPHP
                     ASGPTTIALAPDGTVWFTESNGNRIGRMNPDGSGLVEYDVPTEESSPRIIALGADGNM
                     WFSEHLAGQMARITPQGEIAEFRIATPDSAPRAIALGADGNIWFGMFAAGKIGRITPA
                     GAITEFDLPTPFSGPRALAAGPDGNIWFSEFHASKIGRITMTGTVTEFPLPRPDSGPG
                     DITAGADGNMWFVELNGTMDGRVVSGNRVGRVSMRGEITEFAIPSATGSPTNVAVGPD
                     RNIWYTKGASLGRVTEAGVITEFPLSANSRGVGLTAGSDRQPPERIANRLWYADGNGN
                     KIGYLHFD"
     gene            2548..3252
                     /locus_tag="VM692_03985"
     CDS             2548..3252
                     /locus_tag="VM692_03985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HUQ51356.1"
                     /translation="MRHLPLAIASLAIVVACTPQESSAPTPAATSSTETSSLQTDQDK
                     TLYALGLVIGGNLSQFSLKSDEIDHVIDGLRDSVLAKEPKVALNEYGPRIQLLAQERA
                     SAASSAEKQASETWVAEQAKAPGAERSATGVVFIPMREGTGTNPTATSTVRVHYHGTL
                     RDGTVFDSSVERNEPISFPLNGVIPCWTEGVQKIKVGGKAKLVCPSETAYGDQGSGSI
                     PPGAALAFEVELLAIE"
BASE COUNT          567 a         1099 c         1080 g          550 t
ORIGIN      
        1 cgtccatgag cttcggcacg agctcccaag cgcggcccgc cttcgcgggg tcgacgccgt
       61 tgatgcggaa catcgtcgac ggatacagca tgttgcccgc gccgtcgccg acctcgtaag
      121 ggacgccggc ccaagcggcg agatcgccgt cgccggagcc gtcgatgaag atcttgccgc
      181 gaattgcaaa gcggcccgac ttggtctcca cgaggaccgc ttcgatttgc ttgtcgttcg
      241 ccatgaccgc gccggtggcg aacgcgtgaa acagcacgtg cacgttggcc tcggcgagca
      301 gctcgtcggc ggcgatcttg aacgccgaaa tgtcgtacgc ctgcgcgaga atctggtttt
      361 gaatcgtcag gtgcggcttg ttcaagccgt ccatgcgctc gagccgggcc aggatgtcgt
      421 cggcaatgcc gtgtacgatt tgctcgtgct tgccgtggac gaccgcgtgc aggccgcaaa
      481 aggtgctcag gcccgcagcc gtgcctgcgc cgccgcagaa gccgtagcgc tcgatgcaga
      541 tcgtcgaccg gccggcgcgt cccgctgcga tcgccgccgc taagccggcc ggtcccccgc
      601 ccaagacgac gacgtcatag tcgccgtaga tgggcgtgtg ccggctgggc tctagtacgt
      661 gcggcgatgg cattcacagt ctctgtcttc gaggttatgg gccaaagttt gcccaggaat
      721 ggtgcgcgag tcacgccgca gcgctctcga gcaccgcagg ctggcgccga gcgcccccgc
      781 tacaacactt tgaacgcgcg aatgatgacg gccccgccct gcgggctcgg cgtgatcgtg
      841 ccgacgaagc tcgccttgtt gagccactca tgcggcccct gcggcgcgtg aaaccgaaca
      901 ttcgtgcgca cgtagcggcc gccgtcggcc gcggccggga cgatgatgcc ttggttgtcg
      961 acctcgatga cggcgccgtc gccggtcttg atcgaataga tcgccttgac ctcggtgacg
     1021 ccgtcggggc ggcgcagctg ccaatcggcg ccgccggcca tgacctcacc tttgaggcta
     1081 ttggcgccgc tgaaacggcc gcccgtgatc gggatgaatt gccgagtgcc gtgatcgctc
     1141 tgtcctacga cgacagcggg tgcgatcgtc acgtagagct cgagcgcgag ctccgtgacg
     1201 ggcgccgtga cgcccgggac gggccagcgc tggccggcat cgtcttgcgc gagcaccgcg
     1261 cccgccagcg ccgcggcgat cagacccaag caaagcgtcg aacgcatggc atgcccccgt
     1321 tgtttaccgg catcttcgca agacgtcgcc ggcggccaca accgctccgt agcgcgccgc
     1381 tgttggccgg ggtctgcgtt gcccgatacc atcacaaatc gccgctcgca gaggtaggca
     1441 tgcgaagaga ggtttcgatc gtggccacgt tgcttgccgc cggttgcgcg tccgcacagc
     1501 agcggtggct caaggacgat gagcgtttga cggcgaccgg cgaagtcaca gagctcgtgt
     1561 tgccgcatcc cgcaagcggc cccacgacga tcgcactcgc gcccgacggc acggtctggt
     1621 tcaccgagag caatggcaac cgcatcggcc gcatgaatcc cgacggcagc gggctcgtcg
     1681 agtacgacgt gccgaccgaa gagagctcgc cgcgcatcat cgctctcggc gcagacggca
     1741 acatgtggtt ctccgagcat ctcgcaggcc agatggcacg catcacgccg caaggcgaga
     1801 tcgccgaatt tcgcatcgca acgccggaca gtgcgccgcg cgcgatcgcg ctcggcgccg
     1861 acggcaatat ttggttcggc atgttcgcgg ccggcaagat cggccgcatc acaccggccg
     1921 gcgcgatcac ggagttcgac ctgccgacgc cgttcagcgg gccgcgcgcg ctcgcggcgg
     1981 gacccgacgg caacatttgg ttctcggagt tccacgccag caagatcggc cgcatcacga
     2041 tgaccggtac cgtgaccgag ttcccgctac cgcggcccga ctcgggcccc ggcgacatca
     2101 cggccggcgc cgacggcaac atgtggttcg tcgagctgaa cggcacgatg gacgggcgcg
     2161 tcgtcagcgg caatcgcgtg ggccgcgttt cgatgcgcgg cgagatcacg gagtttgcga
     2221 ttccgagcgc gacgggctcg ccgacgaatg tcgccgtcgg ccccgatcgc aacatctggt
     2281 acacgaaagg cgcctccctc ggtcgagtga cagaagccgg cgtcatcacg gaattcccgt
     2341 tgtcggcgaa ttcacgcggt gtcggcttga cggccggcag cgaccgtcag ccgccagagc
     2401 gcattgcgaa tcggctttgg tatgcggatg gcaatggcaa caagatcggc tatctgcact
     2461 tcgattagtg cccgccaatt ggctttatcc ggccgattca gctagcgtgc cgggttttcg
     2521 tgccggcgat cggcaccgga gactcccatg cgtcaccttc cactcgccat cgcctcgctc
     2581 gcaatcgtcg tcgcctgcac gcctcaagag tcgagcgcgc cgactcctgc tgccacctcg
     2641 tcgacggaga catcgagctt gcaaaccgat caagacaaaa cgctgtatgc cctgggcctc
     2701 gtgatcggcg gcaacttgag tcagttcagt ttgaagtccg acgagatcga tcacgtcatc
     2761 gacgggctgc gggactccgt gctcgccaag gagccgaagg tcgcgttgaa cgagtacggc
     2821 ccgcgcattc agttgctcgc gcaagagcga gcatcggcgg cctcgagtgc cgagaagcaa
     2881 gcgtccgaaa cttgggtcgc cgagcaagcg aaggcgcccg gcgcggagcg cagcgcgacc
     2941 ggcgtggtgt tcatcccgat gcgcgagggc acaggcacga acccgacggc gactagcacg
     3001 gtgcgcgtcc attatcacgg cactctgcgc gacggcaccg tcttcgacag ctccgtcgag
     3061 cgcaacgagc cgatctcgtt cccgctgaac ggcgtgatcc cgtgttggac cgaaggcgtg
     3121 cagaagatca aggtcggcgg caaagccaag ctcgtctgtc cgtcggagac ggcctacggc
     3181 gaccaaggct cgggctcgat tccgccgggt gctgcgctcg cattcgaagt cgagctgctc
     3241 gcgatcgagt aatccgctgc gctagagaag ctccgcctga atctcgcgga gcttgc
//
LOCUS       DATLEE010000187         2219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3933422, whole genome shotgun sequence.
ACCESSION   DATLEE010000187 DATLEE010000000
VERSION     DATLEE010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3933422"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VM692_03990"
     CDS             complement(<1..207)
                     /locus_tag="VM692_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51357.1"
                     /translation="MSAVLSRAVDDDFCASIHDRSVDELDAAIAGLARQMTSDSYRML
                     VLVREFDDRFGWKKWGCKSCAEWLA"
     gene            400..1326
                     /locus_tag="VM692_03995"
     CDS             400..1326
                     /locus_tag="VM692_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ51358.1"
                     /translation="MTKNLSDVVASAAPQTRDRDSIEAARMRRRTFLGALCAGAGATL
                     LRRHAHGQARVLPLKNLGLEHLDIVVPDTAESARFYKRVFSSPLHEQNFRGGKRYFVL
                     LGDLPADRQVGYIAIGAANGRPIGVGHYCALAESVDLDAIGKELAAAGYPQPAGGFGM
                     IPDLDSLELQLFKPPAGLVTAATPSTLEVGNDGAFTPLGLDHVLLAVADRERALRYYR
                     FLYGEGLETRDAEVPERVWFNLARNTRIGIEPAARGAQPRFVRFGIKVAPFDSAAVAP
                     VLRAAGAEVLAVAPSLIRFRDNYGITLEVVAG"
     gene            complement(1323..>2219)
                     /locus_tag="VM692_04000"
     CDS             complement(1323..>2219)
                     /locus_tag="VM692_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51359.1"
                     /translation="TPWGDLDLQGEWTTEGEYGIPLERPAEFGARAFLTDEEYAKRLA
                     DVQERDERDLKNVDVLSGKVEGPNAPIPHWREYETDSRRTSLVIDPPNGRLPPRTPEA
                     APQVAQRCGSLQRGEPCDSYVDYALGVRCIVHGGGFPDAMFPAVYNANFRIVQAPGVV
                     AITYELIHDTRIIPLDSSPQEEHVSPAIRMYMGDARGHFEGATLVVETTNLKAGTRGA
                     SSDLRLVERFKRVSKDLIQYEVTFVDPRTWTAPWTAALDMKARPADAGVYEYACHEGN
                     YGMRNMLSTARYLERQATSARP"
BASE COUNT          368 a          738 c          713 g          400 t
ORIGIN      
        1 cgccagccac tcggcgcagc tcttacatcc ccatttcttc cacccgaaac ggtcgtcgaa
       61 ctcgcgcacg agcacgagca ttcgatagga gtcggaagtc atctgccgcg cgaggccggc
      121 gatggcggcg tcgagctcgt cgacactgcg gtcatggatc gaggcgcaga aatcgtcgtc
      181 gacagcgcga gacaagacgg cgctcatgga cgtctccggt gattagatca gggatactga
      241 tcatataccc agtagtacgc cgcttggcca attctaatca gctctttaca gcaagtttca
      301 cggggcgcta gtcacacacg gcgttatcga atcgctattg ttcggtgtta ccggtcatga
      361 gcgtgtgtag ttgtttgcgc gtttggacag gggaaagaga tgaccaagaa cttaagcgac
      421 gtcgtggcca gcgctgctcc gcagacccgc gatcgcgaca gcatcgaggc ggcgcgcatg
      481 cggcggcgca cgttcctcgg cgcgttgtgt gccggcgccg gcgctacgct cttgcgtcgg
      541 cacgcgcacg gccaggctcg cgtgctgccg ctgaagaacc tcggcctcga gcacctcgat
      601 atcgtggtgc ccgacacagc tgagtccgcc cgcttctaca agcgcgtgtt ttcctcgccg
      661 ttgcacgagc aaaacttccg cggcggcaag cgctacttcg tgctgcttgg cgatctgccc
      721 gccgaccggc aagtcggcta catcgccatc ggcgcggcga acggccggcc gatcggcgtg
      781 ggacattact gcgcgctcgc cgaatcggtg gacctcgacg ccatcggcaa agagctcgcg
      841 gcggccggtt atccgcagcc ggccggcggc ttcggcatga tcccggatct cgacagcctc
      901 gagctgcaac tcttcaagcc gcccgccggg ctcgtgacgg cagccacgcc gtctacgctc
      961 gaggtcggca atgacggggc gttcacgccg ctcgggctcg accacgtcct gctcgccgtc
     1021 gccgatcggg agcgtgccct gcgctactac cgcttcctgt acggcgaggg gctcgagacg
     1081 cgcgatgccg aggtaccgga gcgcgtgtgg ttcaaccttg cccgcaacac gcgcatcggc
     1141 atcgagcccg ccgcgcgcgg agcgcagccg cggttcgtgc gattcggcat caaggtggcg
     1201 ccgttcgaca gcgcggccgt cgcgccggtg ttgcgagcag ccggcgccga ggtgctcgcg
     1261 gtggcgccgt cgctgatccg cttccgcgac aactacggca tcacactgga agtcgtcgcc
     1321 ggctagggac gcgcagacgt ggcctgtcgc tccaggtaac gcgccgtgga caacatgttg
     1381 cgcatgccgt aattgccctc gtggcaggcg tactcgtaga caccggcatc ggcgggcctt
     1441 gccttcatgt cgagcgcggc cgtccacggc gccgtccacg tgcgcggatc gacaaacgtc
     1501 acctcgtact ggatcaagtc cttcgacacg cgcttgaagc gctcgacgag ccgcaagtct
     1561 gaagaagcgc cgcgcgtgcc ggccttcaag ttcgtggtct cgacgacgag cgttgcacct
     1621 tcgaagtggc cgcgtgcgtc gcccatgtac atcctgatcg ccggcgacac gtgctcctct
     1681 tgcggcgacg agtcgagcgg aatgattcgt gtgtcgtgga tcagctcgta ggtgatcgcg
     1741 acgacgcccg gagcttggac gattcgaaag ttggcgttgt agacggccgg aaacatcgcg
     1801 tccggaaatc cgccgccgtg gacgatgcac cgcaccccga gcgcgtaatc gacatacgag
     1861 tcacacggtt caccgcgttg cagactgccg caccgttggg cgacctgcgg cgctgcttcc
     1921 ggcgtgcgcg gcggcaagcg cccgttcggc ggatcgatca cgagcgaagt gcggcgcgag
     1981 tccgtttcgt actcacgcca atgcggaatc ggtgcgttcg gcccttcgac cttgcccgac
     2041 aacacgtcga cgttcttgag atcacgctcg tcgcgttcct gaacgtcggc caggcgcttc
     2101 gcatactctt cgtcggtcag gaacgcgcga gcgccgaatt ccgcggggcg ctccaacggg
     2161 atgccgtatt ccccttccgt cgtccactcg ccctgaaggt cgaggtcgcc ccaaggcgt
//
LOCUS       DATLEE010000188         4413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1700348, whole genome shotgun sequence.
ACCESSION   DATLEE010000188 DATLEE010000000
VERSION     DATLEE010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700348"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VM692_04005"
     CDS             complement(<1..379)
                     /locus_tag="VM692_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ51360.1"
                     /translation="MSGASLDNELVLVTGGGQGLGAAISRVLARAGALVAVTDIRAER
                     ADAVAREITQGGGKAFALRIDVGDERAAPEAVLAAADAHGGLYALINNAGVDVTLPID
                     ELSVEQWDAVLNTNLRGPFLLCRT"
     gene            complement(376..1671)
                     /locus_tag="VM692_04010"
     CDS             complement(376..1671)
                     /locus_tag="VM692_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ51361.1"
                     /translation="MPQRIAMISDHASPLAAVGGVDSGGQNIYVAETARHLAARGYVV
                     DVFTRKDQPDLPEIVDWVPGVRVIHVPAGPPTFVRKEELLPLMPEFSAFVQRFAAAQR
                     YDVVHAHFFMSGLVALQLKAALGLPFAITFHALGLVRRLHQGVADEFPPKRIGIERDI
                     ITAADAIIAECPDDRRNLVTLYNADPTKIVVIPCGFAPDEFWPIAPRFARRALGLPSD
                     VPVLLCVGRLVPRKGVDNVIRALGVLVRKLGVPAELLIVGGNSDIADPALTPEIGRLR
                     SIAVEEGVADRVTFTGRRSRELLKLYYSAADAFVTTPWYEPFGMTPLEAMACGTPVVG
                     ARVGGIKYSVLDQRTGFLVPPNDPAALAGRLKELCCSPRLAERFAGLAVRRAYALFRW
                     ERVAQLLAILYGDVQRYAEVPATLPVVAPEPVRGVVAAA"
     gene            complement(1825..3123)
                     /locus_tag="VM692_04015"
     CDS             complement(1825..3123)
                     /locus_tag="VM692_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51362.1"
                     /translation="MKRQLCARRIGVCLIFGCACVIQAWRVQGQAQWETVPDIKLQAE
                     SNDNPTLDSGFGVTPGTPSPIDSANRVLADAAIRFRRANPRGELMLEPRVRRDAYANE
                     EAQPLESTDVFFRSSGLKNGQTTRVGYSADLARERIIGLEFLETLDPVDDDPTAVATN
                     QVGANELRTRIGVAPYVDIELNSRTLLRLDGRIVDVDYNSGATAGRTDFLERAIGGEY
                     RKQLSDQRGSLGVHVFATGYEAALNNNTTDTRGIELIFGRELSELWTWNVSGGTQRSD
                     YALSAGGRRIRGTDSSAIFGVGLSKRAEVSTMRVDVRRTMSPDSLGFVAPRDEFRVAW
                     QRMLSAKINGRIVVRAIDAEGIPTVANSERLYGRAELDVEWQFSPTWWITAGYAHSTA
                     QRDVVVNNVTVSNSSDSNALTVGLRYRGKSTQRQPGDLAR"
     gene            complement(3140..>4413)
                     /locus_tag="VM692_04020"
     CDS             complement(3140..>4413)
                     /locus_tag="VM692_04020"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HUQ51363.1"
                     /translation="ALLTLLVMARDAVQQANLVGAQGLTVAGRIGLHWLAVVGVLLLL
                     GYATKSSEDFSRRALFLWFLLTPPFLVAASLGVQQWLRALLVSSRNARSAVIVGSTRM
                     ALELARTLHQRPELGLKLRCVFVDEASGDQAIDERTSAALRNTSGAEIRTQGDVRAYV
                     NARHIDVVFIARQTHAPGVCTLLSDLRDTTTSVYLIPDVSLYDLIQARVGDVDGIPVI
                     ALCESPLQGGRGAMKRFTDIVFSLVMLVVAAPLMALIALAIKLDSPGRVLFKQDRYGL
                     DGERIVVYKFRTMNVCENGPCIVQAKRNDSRVTRVGRILRRTSLDELPQLINVLQGRM
                     SLVGPRPHAVAHNEEYRKLISGYMVRHKVTPGITGLAQVSGCRGETSTVDDMRRRVQY
                     DLEYLRHWCWLLDIKIMLRTLVLLVHDKRAY"
BASE COUNT          767 a         1486 c         1465 g          695 t
ORIGIN      
        1 ccgtgcggca gagaaggaaa gggccgcgta agttcgtgtt cagtaccgcg tcccactgct
       61 cgacgctgag ctcgtcgatc ggcaaggtga catcgacacc ggcgttgttg atcagcgcgt
      121 acaaaccgcc gtgcgcgtcc gctgcggcga gcacggcttc aggcgccgcg cgctcgtcgc
      181 cgacgtcgat gcgcagcgcg aacgccttgc cgccgccttg cgtgatctcg cgcgcgacgg
      241 catccgcacg ttcggcgcgt atgtccgtga cggcgacgag agcacccgcg cgcgcgagaa
      301 cgcggctgat tgcggcaccg agcccttgtc cgccgcccgt tacgagcacg agctcgttgt
      361 cgagcgacgc gccgctcatg ctgccgccac gacgccgcgc acgggctccg gcgcaacaac
      421 cggcagcgtt gccggcactt cggcgtagcg ctgcacgtcg ccgtacagaa tcgccaacag
      481 ctgcgctacg cgctcccagc ggaacagcgc gtacgcccta cgcacggcaa ggcctgcgaa
      541 gcgctccgcg agccgcggtg agcagcacag ctccttgagc cggcccgcga gcgcggcggg
      601 atcgttcggc ggcacgagga accccgtgcg ctggtcgagc acgctgtatt tgatgccgcc
      661 gacgcgcgcg ccgacgacgg gagtcccgca agccattgct tcgagcggcg tcataccgaa
      721 cggctcgtac cacggcgtcg tgacgaaggc gtccgcggcg ctgtagtaga gcttcaacag
      781 ctcgcgcgag cgccgccccg tgaacgtcac tcgatcggcc acaccctcct cgaccgcgat
      841 agaccgcaag cggccgatct cgggcgtcaa tgcaggatct gcgatatcgg agttgccgcc
      901 gacaatcagc agctcggccg gcacgccgag cttgcgcacg aggacgccga gcgcgcggat
      961 cacgttgtcg acgcctttgc gcggcacgag ccgcccgacg cagagcagca ccggaacgtc
     1021 gctcggcagc cccagcgcgc ggcgcgcgaa ccgcggcgcg atcggccaga actcgtcggg
     1081 cgcgaagccg catgggatca cgacgatctt cgtcggatcg gcgttgtaca gcgtcacgag
     1141 attgcggcgg tcgtctgggc actcggcgat gatcgcgtcg gccgccgtaa tgatgtcgcg
     1201 ctcgattccg atgcgcttcg gcgggaattc atcggcgacg ccttgatgca agcggcgcac
     1261 tagacccagc gcgtggaagg taatggcgaa cggcaacccg agcgccgcct tcagctgtag
     1321 agcgacaagg cccgacatga agaagtgagc gtgcacgacg tcgtaacgct gcgcggccgc
     1381 gaagcgctgc acgaacgcac tgaactccgg catcagcggc aggagctcct ccttgcgcac
     1441 gaacgtcggc gggccggccg gcacgtggat cacgcgcacg cctgggaccc agtcgacgat
     1501 ctccggcaaa tcgggttggt ccttgcgcgt gaagacgtcg acgacgtagc cgcgagcagc
     1561 gaggtgccgc gccgtctcgg cgacgtaaat gttctggccg ccgctatcga ctcctccgac
     1621 ggcggcgagc ggcgacgcgt ggtcgctgat catggcaatt ctttgcggca cgcgaacctc
     1681 cttgtaccgt ccgtggcaaa gcggtgtggg gtgcctctgg agaaccgttg ctcggccgag
     1741 cgatgcagcc acccgccggc acgacgtgca aagcgcgtgc cacgcgcgcg aaaagctctc
     1801 ggactctccg ccgcgaagac gaggtcagcg cgcgagatcg cccggttggc gttgcgtcga
     1861 cttgccgcga tagcggaggc cgaccgtcaa cgcgttcgag tcggagctgt tgctgaccgt
     1921 tacattgttg acgacgacgt cgcgctgcgc agtcgaatgc gcatagcccg cagtgatcca
     1981 ccaggtcggg ctgaactgcc actcgacgtc gagctccgcg cggccgtaga gacgctcgct
     2041 gttcgcgacc gtcggaatgc cttcggcgtc gatcgcacga acgacgatgc ggccgttgat
     2101 cttcgccgac agcatgcgtt gccacgccac gcgaaactcg tcgcgcggcg cgacgaagcc
     2161 gagcgagtcg gggctcatcg tgcggcggac gtcgacgcgc atcgtcgaga cttcggcgcg
     2221 cttcgagagg ccgactccga agatcgcgct gctgtcggtg ccgcgaatgc gtcggccgcc
     2281 ggccgacaag gcatagtcgg aacgctgcgt gccgccgctg acgttccacg tccagagctc
     2341 cgacaactca cgcccgaaga tcagctcgat gccgcgtgtg tcggtcgtgt tgttgttcag
     2401 cgcggcttcg taaccggtcg caaatacgtg cacgccgagc gagccgcgtt gatcggagag
     2461 ctgcttgcga tactcaccgc cgatcgcgcg ttcgaggaag tccgtgcgcc cggccgtcgc
     2521 gccgctgttg tagtcgacgt cgacgatgcg cccgtcgagg cgcagcagcg tgcggctgtt
     2581 gagctcgatg tcgacgtacg gcgccacacc gatgcgcgtg cgtagctcgt tggcgccgac
     2641 ctggttcgtc gcgacggccg tcgggtcgtc gtcgacggga tcgagcgtct cgaggaactc
     2701 gagaccgatg atccgctcgc gcgcgaggtc ggccgagtac cccacgcgcg tcgtctgacc
     2761 attcttcaga ccgctcgaac ggaagaagac gtcggtgctc tcgagcggct gcgcctcctc
     2821 gttcgcgtac gcgtcgcgcc ggacgcgcgg ctcgagcatg agctcgccgc gcggattggc
     2881 gcgccgaaag cggatcgcag cgtcggcgag cacgcggttg gccgagtcga tcggcgacgg
     2941 cgtcccgggc gtcaccccga agcccgagtc cagcgttgga ttgtcgttgc tctccgcctg
     3001 caacttgatg tcggggaccg tttcccactg agcctgacct tggacgcgcc acgcctgaat
     3061 cacgcacgcg cagccaaaga tcagacacac gccgatgcgg cgtgcgcaca gctgacgttt
     3121 cattgtcgaa cctccctaat cagtacgcgc gcttgtcgtg aacgagtagc accaacgtgc
     3181 gtagcatgat cttgatgtcg agcaaccagc accaatggcg caagtactcg aggtcgtact
     3241 ggacgcggcg acgcatgtcg tcgacggtcg acgtttcgcc gcggcagccg ctgacctgtg
     3301 ccaagcccgt gatgcccggc gtaactttgt gccgcaccat gtagcccgag atcagcttgc
     3361 ggtattcctc gttgtgcgcg acggcatgcg gtcgcgggcc gacgaggctc atgcgccctt
     3421 gcaagacgtt gatcagctgc ggcagctcgt cgagcgaggt gcgccggagg atgcggccca
     3481 ctcgcgtgac gcgcgaatcg ttgcgcttgg cttgcacgat gcagggcccg ttctcgcaga
     3541 cgttcatcgt gcggaacttg tagacgacga tgcgctcgcc atcgagcccg tagcggtcct
     3601 gcttgaacag cactctgccg ggcgagtcga gcttgatcgc gagagcgatc aacgccatca
     3661 gcggcgcagc gacgaccagc atcacgagcg agaacacgat gtcagtgaat cgcttcatcg
     3721 caccgcgtcc gccttgcagc ggcgactcgc atagcgcgat gacgggaatc ccgtcgacgt
     3781 cgccgacgcg cgcttgaatc aagtcgtaca acgacacgtc tggaatcaag tagacagacg
     3841 tcgtcgtgtc gcgcagatcg ctgagcagcg tgcaaacccc gggggcgtgc gtttgccggg
     3901 cgatgaatac gacgtcgatg tgccgcgcgt tcacataggc acgcacgtcg ccctgcgtac
     3961 ggatctcggc gcccgacgtg ttgcggagcg ccgcgctggt cctctcgtcg atggcctggt
     4021 cgccggacgc ttcgtcgacg aacacgcagc gcaacttgag ccccagctcc gggcgttggt
     4081 gcagcgtccg cgcgagctcg agcgccatgc gcgtcgaacc gacaatgacg gcgctgcgcg
     4141 cgttgcgcga ggaaacgagc aacgcccgca gccactgctg aacgccgagc gacgccgcga
     4201 caagaaacgg cggcgtcaac aagaaccaca ggaacaaggc gcgccgtgaa aaatcttccg
     4261 agctcttcgt cgcatagccg agcagcagca acaccccgac tacggcgagc cagtgcagac
     4321 cgattcgtcc cgccaccgtc agcccctggg caccgacgag gttcgcctgc tgcacggcgt
     4381 cgcgcgccat gacgagcaac gtcaacagcg ccg
//
LOCUS       DATLEE010000189         4148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6067037, whole genome shotgun sequence.
ACCESSION   DATLEE010000189 DATLEE010000000
VERSION     DATLEE010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6067037"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..682)
                     /locus_tag="VM692_04025"
     CDS             complement(14..682)
                     /locus_tag="VM692_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HUQ51364.1"
                     /translation="MKLAIFDIDGTLVEGSTERRFWRYLLKRGHQGPRQVLAYLVFWL
                     RYFPVYRGQTAKKNKAYLYKLGTARVRALAERFVAEEILPRLYAPAVQRLQSHLRRGD
                     TVVLLSGTLEPIARALAKALAVEHVRATVCRERGERYLAGPPTVHPFGAAKLEIAVQF
                     AAEVGADLREASAYGDSRHDLTLLEAVGDPVAVLPDRPLLETARGNRWLIIRSRNAPH
                     VFPQ"
     gene            complement(679..1311)
                     /locus_tag="VM692_04030"
     CDS             complement(679..1311)
                     /locus_tag="VM692_04030"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUQ51365.1"
                     /translation="MANLVTLSRLLLLLVVVWLFYQPPSWWSLASFFLIVFIFVSDGL
                     DGYIARKRNETSLFGALFDIAGDRITELTLWIVAADTDLVPIWVPLVFIIRGVIVDTI
                     RSSNAVAEGVAPFALMRSTLGKFIVAGKFMRILYAVAKAAAFSGLALQHPAPVLLPAF
                     WDYIGWLLTWLTYFFVYLSVVLCIARGLPVIAEFVQSQKHDILRRPGRKQ"
     gene            1384..2028
                     /locus_tag="VM692_04035"
     CDS             1384..2028
                     /locus_tag="VM692_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUQ51366.1"
                     /translation="MRFYDYRLAPSPRKVRLFIAEKGLDIPAVEVDLRARAQHEPDFL
                     AKNPGATVPVLELDDGTCLTESLAICHYLEQLRPDPNLMGRGAKEQALVLMWTDVQTF
                     EGYLGLAEALRNGHDAFKGRGLGGPVSYEQIPALAQRGKRRAEIYFNKLETRLGESRF
                     IASDRYTYADIVGYVYVGFAKRALGEAAAPLEGRPALKAWHDTIAARPAIRGAS"
     gene            complement(2025..2822)
                     /locus_tag="VM692_04040"
     CDS             complement(2025..2822)
                     /locus_tag="VM692_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ51367.1"
                     /translation="MTLAAAAIEVRYDARTAVRPTTVALEPGQLVALVGPNGAGKSTL
                     LRALAGLVAHLGSVTWRGTSLTRLDGGSRARTVAYLPQDPPVHWPLAAREVVALGRLP
                     HRAYGAEPKPADDDAVAGAMRQTDTLAFAERSVQQLSVGERARVLLARALAVEAPVLL
                     VDEPIAMLDPYHQLDVMRTLRAYAGAGAEPRLVVAVLHDLGLAARFCDRVLLMHDGVV
                     VGDGPPDATLSSAAIRAHYSVEPYIARHDGEPLVVPWRALRRAGDAD"
     gene            complement(2830..3831)
                     /locus_tag="VM692_04045"
     CDS             complement(2830..3831)
                     /locus_tag="VM692_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HUQ51368.1"
                     /translation="MSTAVARAGPSQWTLGLGLAALVLLAAGASLVIGRDGGGPTFGF
                     GGPELMDVIFKELRLPRTMLAVLVGGTLGLCGAALQGLLRNPLAEPGLLGASSGAALG
                     AVIMFYFGLAGGSSLLLPLSAVAGSLLALGALYALAGRRGDFLTIVLAGIAINALAAA
                     LTSLALNFAPSPYAALEILFWMLGSLADRSLAHVYLALPLMVPGWLLVAAAARGLDAL
                     TLGEETASSLGFAPQRTQWLVIGGTALAVGGAVAVTGVVGFVGLVVPHLLRRTVGQHP
                     SRLLLPSFLGGALLTTLADVAIRLLPPGPELKLGVVTALLGAPFFLHLVMRGSARAA"
     gene            complement(3828..>4148)
                     /locus_tag="VM692_04050"
     CDS             complement(3828..>4148)
                     /locus_tag="VM692_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUQ51369.1"
                     /translation="GFTVGADSLSHELLGLAGLRNVAAEQGLDRWGSLSMEALLRSAP
                     ELIVLTGYRSTQPSLANAVLEHPALRKLGAVHRTVTVPSALWSCGLPRSVASAVLLQR
                     AAQQ"
BASE COUNT          742 a         1422 c         1422 g          562 t
ORIGIN      
        1 catgtagccg ccgctactgc gggaacacgt gaggggcgtt gcggctgcgg atgatgagcc
       61 aacggttgcc gcgcgccgtt tccagcaaag ggcggtccgg cagcaccgcg acggggtcgc
      121 cgacggcctc gagcagcgtc aggtcgtgcc gtgagtcgcc ataagcgctc gcttcacgca
      181 agtccgctcc gacctcggca gcgaattgca cggcgatctc gagcttagcc gcgccgaagg
      241 ggtgcaccgt cggcggtccc gccaagtacc gctcgccgcg ttctcggcat accgtcgcgc
      301 gcacgtgctc gaccgccaac gctttggcga gcgctcgagc gatcggctcg agcgtgcccg
      361 acagcagcac tacagtgtcg ccgcgccgca agtggctctg gaggcgctgc actgccggtg
      421 cgtagagacg cggcaggatt tcctcggcga cgaagcgctc cgcgagggca cgtacccgcg
      481 cggtgccgag cttgtacaga tacgccttgt tcttcttggc cgtctggccg cggtagacgg
      541 gaaagtaccg cagccaaaac actaggtacg cgagcacctg acgtggcccc tggtggccgc
      601 gcttcagcag gtagcgccag aaccgacgtt ccgtgctgcc ttcgacgagc gttccgtcga
      661 tgtcgaagat tgcaagcttc attgctttct gcccggtctc ctcaggatat cgtgcttttg
      721 cgattggaca aactcggcga tgaccggcag gccgcgcgcg atgcagagca ctacggacag
      781 atagacgaag aaataggtca gccacgtcaa cagccagccg atgtagtccc agaacgcggg
      841 caatagcacg ggagccggat gctgcagcgc caaccctgag aatgcagcag ccttcgcgac
      901 ggcatacaag atccgcatga acttgccggc gacgatgaac ttgccgagcg tcgagcgcat
      961 cagcgcgaac ggagcgacgc cttccgcgac ggcattgctc gatctgatcg tgtcgacgat
     1021 gacgccgcgg atgatgaaaa ccagcggtac ccagatcggc acgaggtccg tgtcggcggc
     1081 gacgatccac agcgtgagct cggtgatgcg atccccggca atgtcgaaca gcgcgccgaa
     1141 caagctggtc tcgttgcgct tgcgcgcgat atagccgtcg agcccgtccg acacgaagat
     1201 gaagacgatc aggaagaagc tggccaacga ccaccaactc ggcggttgat agaacagcca
     1261 tacgacgacg agcagcagca ggagccggct cagtgtcacg aggtttgcca tggaccaatt
     1321 atacgaagga cggcgcgtcg ctcgcgccag ttagactaat gccgagtttg gcaggggcaa
     1381 cgcatgcgct tttacgacta tcggctcgcg ccgagcccgc ggaaggtacg actgttcatc
     1441 gccgaaaaag gcctcgacat accggccgtc gaagtcgact tgcgggcccg agcccagcat
     1501 gagcccgact ttctcgcgaa gaaccccggc gcaacggtgc ccgtgctcga gctcgacgac
     1561 ggcacgtgcc tgacggaaag cctcgcgatc tgccactacc tcgagcagct gcgccccgac
     1621 cctaacctca tggggcgcgg cgccaaggag caagcgctcg tgctcatgtg gaccgacgtc
     1681 caaacgttcg agggctacct cggcttggcc gaagcgctgc gcaacggtca cgacgctttc
     1741 aagggccgcg ggctcggcgg ccctgtgtcg tatgagcaga ttcccgcgct cgcgcagcgc
     1801 ggcaaacgcc gcgccgagat ttatttcaac aagctcgaga cgcggctcgg cgagagccgg
     1861 ttcatcgcca gcgatcgcta cacgtacgcc gatatcgtcg gctacgtcta cgtcggcttc
     1921 gcgaaacgcg cgctcggcga agccgccgcg ccgctcgagg gccgtcccgc gctcaaggcg
     1981 tggcacgaca cgatcgcggc tcgaccggcc attcgcggcg cgagctagtc ggcgtcgccg
     2041 gcccgccgca gcgcgcgcca aggaacgacc agcggctcgc cgtcgtggcg cgcgatgtac
     2101 ggctcgacac tgtagtgcgc acgaatggcg gccgagctca acgttgcgtc gggtgggccg
     2161 tcgccgacga cgacgccgtc atgcatcagc agcacgcgat cgcaaaagcg tgcggccagc
     2221 cccaaatcgt gcagcacggc gacgacgagc cgcggctccg cgccggcgcc ggcgtacgcg
     2281 cgcagcgtgc gcatgacgtc gagctgatgg taagggtcga gcatcgcgat cggctcgtcg
     2341 actagcagca cgggtgcttc gacggcgagc gcgcgggcca acagcacgcg tgcgcgttcg
     2401 ccgaccgaca gttgctgcac gctgcgctcc gcgaacgcga gcgtgtcggt ttggcgcatc
     2461 gcgccggcga cggcgtcgtc gtccgcgggc ttcggctccg caccgtaggc gcgatgcggc
     2521 agccggccaa gcgcgacgac ctcccgagct gcgagcggcc aatggacggg cgggtcttgc
     2581 ggcaagtaag cgacggtgcg cgcgcggctt ccgccgtcga gccgcgtcag cgacgtgccg
     2641 cgccaagtta cgctgcccaa gtgcgcgacg agcccggcga gagcgcgcag cagcgtgctc
     2701 ttgccggcgc cgttcggtcc gacgagcgct acgagctggc ccggctcgag ggcaacggtc
     2761 gtcggtcgca cggcagtgcg tgcgtcgtag cgaacctcga ttgcggcggc cgccagcgtc
     2821 attgctctgt cacgctgcgc gcgccgagcc gcgcatcacg aggtgcagaa agaacggcgc
     2881 gccgagcagc gccgtgacga cgccgagctt gagctcgggg cccggcggca atagtcgaat
     2941 tgcgacatcg gcgagcgtcg tcagcagcgc gccgccgaga aagctcggca gcagcagccg
     3001 actcgggtgt tggccgacgg tgcggcgcag caaatgcggc acgacgaggc cgacgaagcc
     3061 gacgacgcct gtcaccgcga ctgcgccgcc gacggcgagc gccgtgccgc cgatcacgag
     3121 ccactgcgtg cgctgcggcg cgaagccgag gctcgatgcc gtctcttcgc cgagcgtcag
     3181 cgcgtcgagc ccacgcgcgg cggcggcgac gagcagccag cccggcacca tcagcggcag
     3241 cgcgagatag acgtgcgcga ggctgcggtc tgccagtgag ccgagcatcc agaacaggat
     3301 ctcgagcgcg gcgtacgggc tcggcgcgaa gttcagcgcg agcgacgtca gcgcggccgc
     3361 aagcgcgttg atcgcgatgc cggccagcac gatcgtcagg aaatcgccgc gccggcccgc
     3421 aagcgcgtac agcgcgccga gcgcgagcag cgagccggcg accgcgctga gcggcagcag
     3481 cagcgacgag ccaccggcga gcccgaagta gaacatgatg acggcgccga gcgcggcacc
     3541 gctcgatgcg ccgagcaacc ccggctccgc gagtggattg cgcagcaacc cttgcagcgc
     3601 cgcgccgcac aagccgagcg tgccgccgac gagcaccgcg agcatcgttc gcggtaggcg
     3661 cagctccttg aaaatgacgt ccatgagctc cggcccgccg aagccgaacg tcggtccgcc
     3721 gccgtcccgg ccgatcacga gcgaagcgcc ggccgcgagc agcacgagcg cggcgagccc
     3781 tagcccaagc gtccactgcg acggtccggc ccgtgcgact gccgtgctca ttgttgcgcc
     3841 gcgcgctgga gcagcacggc cgacgcgacg ctgcgcggca gcccgcacga ccatagcgcc
     3901 gacggcacgg tcaccgtccg gtgcacggcg ccgagcttgc gcagggcagg gtgctcgagc
     3961 accgcgttgg ccagcgacgg ctgcgtgctg cgatagcccg tcaacacgat gagctccggc
     4021 gcgctgcgca gcagcgcttc catcgacagg ctcccccacc ggtccaagcc ttgctcggcg
     4081 gccacgttgc gcaggcctgc gagaccgagc agctcgtgcg aaagggagtc ggccccgacc
     4141 gtgaagcc
//
LOCUS       DATLEE010000190         3568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4675578, whole genome shotgun sequence.
ACCESSION   DATLEE010000190 DATLEE010000000
VERSION     DATLEE010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4675578"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VM692_04055"
     CDS             <1..924
                     /locus_tag="VM692_04055"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ51370.1"
                     /translation="VHAHCLFPEVAQRVPNVAVPRAAPPTLVLGPERIAAMDERGIDL
                     QALSVNTYWWYSASRADASAVVAMHDEGIAAWCKEHPTRFVGLSSVALQYPDLAAEQL
                     EHAVKSLGARGASIGGHVSNEPPTSEKYDPFWAKAEELGVPVFMHPNNALNIAREGSL
                     DGRGGLGNIVGNPLETTVFLSRMIFDGVLDRFPRLTVCAAHGGGFITSYLGRSDAACV
                     VRDDANCANKRKPSAYFKDQIVVDSMVFSAEGLRHLVAETGVSQVVYGSDQPYFWPDT
                     IDVIAEASFLSDADKEAILGGNLRRLLRIGA"
     gene            932..1354
                     /locus_tag="VM692_04060"
     CDS             932..1354
                     /locus_tag="VM692_04060"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUQ51371.1"
                     /translation="MAALRYLLFAAVGVAIASLLFLVMNFIVTANFDAGVGVVERWIV
                     RATCEQYELSGTVRGTAGRPVPFAVVEVSYPDERLTTRSNSDGTFMLAAAAPVCDRRP
                     PASVQLVVVADGFRPKTQAVPFETASIEVTLDARDFRP"
     gene            1370..1837
                     /locus_tag="VM692_04065"
     CDS             1370..1837
                     /locus_tag="VM692_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51372.1"
                     /translation="MSTKLFVALCGAVTIAWLPSAGAQDDEERQISSEPLQCLSMTSI
                     RSTRVVDDSTVLFFQSRRVYVNRLERECLGLTRSGRFTYQVQTGARQARLCATDSITV
                     LESTGRGFNCGLGKFREVSREEADAFLIGPNRAVTSTQVELPKSEEPAPPAKQ"
     gene            1873..3210
                     /locus_tag="VM692_04070"
     CDS             1873..3210
                     /locus_tag="VM692_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017748125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HUQ51373.1"
                     /translation="MTDSLNRRDFIGAAAGAAAATVAVPRAVRAQQRPNVLFILADDL
                     GYGDLSCYGRPDYDTPVLDRLATQGMKFMSAYAAAPVCTPTRCAFMTGRYPQRFEVGL
                     QEPLTVRSPPVGLPQAQPTIATRMRAAGYETALIGKWHLGWRPEYQPNRFGFDEFFGS
                     LSGALDYFTHVAPDADIGTEPDLWENDRRTTTDGYLTDVFTDRAIDYVTRRRSKPFYL
                     SLHYTAPHSPWEGPDDRAEVDHDDHGPGPMTNGGSLGVYAEMMRSMDAGIGRVLAALR
                     DARLERNTLVIFTSDNGGERYSFNWPFSSEKFSLYEGGIRVPAIVRWPGVVPAGAATQ
                     QAAITMDWTATILAAAGAPADAAPLDGDSLLPVLTGEREVYDRVLFWRTRERAAARIG
                     RWKYVQEGADDHLYDIGVDLGEKTDLKTREPAAFEDVKGRYTAWAAQMLPRPT"
     gene            3216..>3568
                     /locus_tag="VM692_04075"
     CDS             3216..>3568
                     /locus_tag="VM692_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HUQ51374.1"
                     /translation="MKTKLLLILLAVLPARVMSQPSFDVMETTVAQIHRAYASGTLTA
                     RQLVQLYLDRIEAYDRNGPRINSVITLNAAALDEADRLDNAYRTSGLVGPLHGIPVML
                     KDQIDARGMPTTLGSV"
BASE COUNT          592 a         1198 c         1181 g          597 t
ORIGIN      
        1 gtgcacgcgc actgcctctt tcccgaggtg gcgcagcgcg tgccgaacgt ggccgtgccg
       61 cgagcggcgc ccccgacact cgtgctcggc cccgagcgca ttgccgcgat ggacgagcgc
      121 ggcatcgacc tccaggcgct cagcgtcaac acctattggt ggtactcggc gagccgcgcc
      181 gatgcgagcg ccgtcgtcgc catgcacgac gaaggcatcg cggcgtggtg caaggagcac
      241 ccgacgcggt tcgtcggctt gagctccgtc gctctgcaat atccggatct cgcggccgag
      301 cagctcgagc acgcggtcaa gagcctgggc gcacgcggcg cctcgatcgg cggtcacgtc
      361 agcaacgagc cgccgacgag tgagaagtac gatccgttct gggccaaggc cgaggagctt
      421 ggcgtgccgg tgttcatgca cccgaacaac gcgttgaaca tcgcgcgcga gggctcgctc
      481 gacggccgcg ggggcttggg caacattgta ggcaatccgc tcgagacgac ggtgttcttg
      541 agccgtatga tcttcgacgg cgtgctcgac cgcttcccgc gcctcacggt atgtgcagcg
      601 cacggcggcg gtttcatcac gtcgtacttg ggtcgcagcg acgcagcctg cgtcgtgcgc
      661 gacgacgcga actgcgcgaa taagcgcaag ccgagcgcct acttcaagga tcagatcgtc
      721 gtcgactcga tggtgttttc ggctgaaggt ctgcgccacc tcgttgccga gaccggcgtg
      781 agccaagtcg tctacggctc ggaccaaccc tatttctggc ccgacacgat cgacgtcatc
      841 gcggaagcct cgttcttgag cgacgccgac aaggaagcga tcttgggcgg caacctacgg
      901 cgcttgttgc gcatcggagc ctgacgaacc gatggcggcg ctgcgttatc tgctgttcgc
      961 cgccgtcggg gtcgcgatcg cctcgctgct gttcttggtg atgaacttca tcgtcaccgc
     1021 caatttcgac gccggcgtcg gcgtcgtgga gcgctggatc gtgcgggcga cctgcgagca
     1081 atacgagctc tcgggcaccg tgcgcggcac agccggtcgg cccgtgccgt ttgccgtcgt
     1141 cgaggtgtcg tatcccgacg agcgattgac gacgcgcagc aacagcgacg gcacgttcat
     1201 gcttgccgcg gcggcgcccg tctgcgaccg gcgcccgccc gccagcgtgc agctcgtcgt
     1261 cgtcgccgac ggcttccggc ccaaaacgca ggccgtgcct ttcgagacgg catcgatcga
     1321 ggttacgctg gacgctcgcg atttcaggcc ctgaccgtta gggggtggca tgagcacgaa
     1381 gttgttcgtt gcgctctgcg gcgcggtgac gattgcatgg ttaccctcgg cgggggcgca
     1441 agacgacgag gagcgccaaa tttcgtcgga gccgctccag tgcctgtcga tgacgagcat
     1501 tcgctcgacg cgagtcgtcg acgacagcac ggtcctgttc ttccagagcc gccgcgtcta
     1561 cgtcaatcga ctcgagcgcg agtgcttggg gctcacgcgc agcggcaggt tcacgtatca
     1621 ggtgcaaacc ggcgcgcgac aggcgcgcct ctgtgcgacc gactcgatca cagtgctcga
     1681 aagcaccggc cgcggcttca actgcgggct cggcaagttc cgggaagtgt cacgcgaaga
     1741 agccgatgcg tttctcatcg gcccgaatcg tgccgtgacg tcgacccaag tcgagctgcc
     1801 gaagagcgag gaaccggctc cgccggccaa gcaataacga ggatcgcttc gccggcgtaa
     1861 cgcgggggca gcatgacgga tagcctgaat cgtcgcgact tcatcggcgc cgcggccgga
     1921 gccgccgcgg caacggtggc cgtgccgcgc gccgtgcgcg cccagcagcg gccgaacgtg
     1981 ctgttcatct tggccgacga tctcggctac ggcgacttga gctgctacgg ccggcccgac
     2041 tacgacacgc cggtgctcga tcggctcgcg actcagggca tgaagttcat gagcgcttac
     2101 gcggcggcgc cggtgtgcac gccgactcgc tgcgcgttca tgacgggccg ttatccgcag
     2161 cgcttcgagg tcgggttgca agagccgctg acggtgagat ctccccccgt cggcctgccg
     2221 caagcgcagc cgacgatcgc tacgcgcatg cgcgccgccg ggtacgaaac ggcgctgatc
     2281 ggcaagtggc atttgggctg gcggcccgag tatcagccga atcgtttcgg cttcgacgag
     2341 ttcttcggca gcctcagcgg cgcgctcgac tacttcacgc acgtcgctcc cgacgccgac
     2401 atcggcaccg agcccgactt gtgggagaac gatcgccgca cgacgaccga cggctacctg
     2461 accgacgtct tcaccgaccg agccatcgat tacgtaacgc ggcggcgcag caagccgttc
     2521 tacctgagcc tgcactacac cgcgccgcac tcgccatggg agggccccga cgatcgcgcc
     2581 gaggtcgacc acgacgatca cgggccgggg cccatgacga acggcggctc gcttggcgtt
     2641 tacgccgaaa tgatgcgcag catggacgct ggcatcggcc gcgttctcgc ggcgctgcgc
     2701 gatgcgcgac tcgagcgcaa tacgctcgtg atcttcacga gtgacaacgg cggcgagcgt
     2761 tactcgttca attggccgtt ctcgtccgag aagttctctc tatacgaagg cggtatccgc
     2821 gttcccgcga tcgtgcgttg gcctggtgtc gtgccggccg gcgccgccac gcagcaggcg
     2881 gccatcacaa tggactggac ggccacgatc ctagccgcgg ccggcgcgcc cgccgacgct
     2941 gcgccgctcg atggcgacag cctgttgccc gtgttgaccg gcgagcgcga ggtctacgat
     3001 cgcgtgttgt tctggcgcac gcgcgaacgc gctgccgccc gcatcggccg ctggaaatac
     3061 gtgcaggagg gcgccgacga ccacctctac gacatcggcg tcgatctcgg cgagaagacg
     3121 gatttgaaga cgcgcgagcc ggccgcattc gaagacgtca aaggccgtta tacggcgtgg
     3181 gcggcgcaga tgctgccgcg gccaacgtga gccccatgaa aacgaaactc ctgttgatcc
     3241 tgctcgccgt gctcccggca cgcgtgatga gtcagccgtc gttcgacgtc atggagacga
     3301 ccgtcgcgca aatccaccgt gcctacgcct ccggaacgct cacggcgcgg cagctcgtgc
     3361 agctctatct cgatcggatc gaggcctacg accggaacgg cccgcgcatc aattccgtca
     3421 tcacgctcaa tgcggccgcg ctcgacgagg cggaccggct cgacaacgct taccgcacgt
     3481 ccggtctcgt gggcccactc cacggcatcc ccgtgatgct gaaggaccag atcgacgcgc
     3541 gtgggatgcc gacgacgctg ggctcagt
//
LOCUS       DATLEE010000191         3230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2449072, whole genome shotgun sequence.
ACCESSION   DATLEE010000191 DATLEE010000000
VERSION     DATLEE010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449072"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VM692_04080"
     CDS             <1..509
                     /locus_tag="VM692_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ51375.1"
                     /translation="APLEMTHTVFMIDEPEKVVPFYTERLGFVASDTYPGRGYFLRGG
                     ASNHHHNLFLLNVGKKRGFHHLAFELSSIHELFGGGLNMTRRGWRTALGPGRHPISSC
                     YFWYFHSPCGGAAEYDFDSDLVDERWVPGTFQQTPEVFAEWCLAEGMADSLLYKGVQT
                     GNTIKDKS"
     gene            543..938
                     /gene="ligA"
                     /locus_tag="VM692_04085"
     CDS             543..938
                     /gene="ligA"
                     /locus_tag="VM692_04085"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HUQ51376.1"
                     /translation="MSKQPEQYEDIPGTWVFDAQRARIGYHLNEFLYSLMKADNRAEF
                     LADERKYLMKFAMTEEQREAVVQRAWNRLLELGGVSYALAKLAFTDKKSYQFMASQMV
                     GIPESEYVKMMLAGGRSPDGWRSKSERKG"
     gene            938..1777
                     /locus_tag="VM692_04090"
     CDS             938..1777
                     /locus_tag="VM692_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HUQ51377.1"
                     /translation="MAKIIAGVGTSHTPAIGAAVDNGKTTEPYWTPLFKGYEPAKKWM
                     AETKPDVAIIVYNDHVNAFDFKIIPTFALGCGAQFPIADEGWGARPVPVVKGYPELAA
                     HMVQSLVLDEFDMTIVNEMQVDHGLTVPMTLLFGQPQAWPVRVIPLAVNVIQYPAPTG
                     NRCYQLGKAIKKAVDTWDEDLRVVIFGTGGMSHQIQGPRAGLINTEFDTAFLDDLTRN
                     PERLARIAPLEYLRDAGAEAIELVMWLIMRGALGPRAREVYRFYHVPASSTGVGNIIL
                     EAA"
     gene            1797..2636
                     /locus_tag="VM692_04095"
     CDS             1797..2636
                     /locus_tag="VM692_04095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ51378.1"
                     /translation="MSAPLTIERYTAPERFLAAAQEWLLEAEVENNLLLGIALNLRGR
                     EAREPRPYWLIVRADERIVACACRTPPNPLVLSHAPATAIARLATELSAADPALSGVT
                     GPETESAAFASAWTAQRGGHPRTRFRLRLHELSHVTFAGVAKGGLRKATTADLALARE
                     WTDAYVRDTGITPSVDIAQRLIDREQLFLWIDGREPRAMVAATRETRSGCSINGVYTP
                     PHFRRHGYATAAVAALSGERLAGGLRFCCLYTDATNPTSNSIYAKIGYRPIRDDVEIA
                     FDA"
     gene            complement(2639..>3230)
                     /locus_tag="VM692_04100"
     CDS             complement(2639..>3230)
                     /locus_tag="VM692_04100"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HUQ51379.1"
                     /translation="RGSMVTAAAMLLAAMTASSQQPSGDGPRYENGTDLVRPADYRSW
                     PFIGAGLGMTYDGERGTQAATQDDPRFTHAFVNPTSYQHFMRTGTWPDGTVFMLEFRG
                     SQTEGSINRAGRFATQLTFLEAEVKDSRFPDGWAFYAFGPGNRLAPAAKPLAGADVAP
                     CVQCHSENGAVERTFVQFYPHLLDVAREKGTLKPGH"
BASE COUNT          573 a         1067 c         1054 g          536 t
ORIGIN      
        1 cggcgccgct cgagatgacg cacacggtct tcatgatcga cgagccagag aaagtcgttc
       61 cgttctacac ggagcgcttg ggcttcgttg cgagcgacac gtaccccggc cgcggttact
      121 tcctgcgcgg cggcgcctcg aaccatcatc acaatctgtt tctgctcaac gtcggcaaga
      181 agcgcggctt ccatcacctc gcattcgagc tgtcgagcat ccacgagctg ttcggcggcg
      241 ggctcaacat gacacgccgc ggctggcgca cggcgctcgg gcccgggcgt cacccgatct
      301 cgtcgtgcta cttctggtac ttccacagcc cgtgcggcgg cgcggccgag tacgacttcg
      361 actcggacct cgtcgacgag cgctgggtgc ccggcacgtt ccagcaaact ccggaagtgt
      421 tcgccgagtg gtgcctcgcc gaaggcatgg cggactcgct gctctataaa ggcgtgcaga
      481 cgggcaacac gatcaaagac aagagttgac actggcgcag cggaacgggt cgaatcacca
      541 ccatgagcaa gcaaccggaa cagtacgagg atatccccgg cacgtgggtg ttcgacgccc
      601 agcgcgcgcg catcggttac cacttgaacg agttcctcta ctcgttgatg aaggccgaca
      661 accgtgccga gttcctggcc gacgagcgca agtacctcat gaagtttgcg atgacggagg
      721 agcagcgcga agccgtcgtg cagcgcgcct ggaaccggct gctcgagctc ggcggcgtgt
      781 cgtatgcgct cgccaagctc gccttcacgg acaagaagtc ttatcagttc atggcgtcgc
      841 agatggtcgg aatccccgag agcgagtacg tgaagatgat gctggccggc ggccgctcac
      901 cggatggttg gcggtcgaag tccgagagga aaggctgatg gcgaagatca tcgccggcgt
      961 cgggacctcg cacacgcccg cgatcggcgc ggccgtcgac aacggcaaga ccacggagcc
     1021 gtactggacg ccgttgttca agggctacga gccggcgaag aagtggatgg ccgagacgaa
     1081 gccggacgtg gcgatcattg tctacaacga ccacgtcaac gcattcgatt tcaaaattat
     1141 tccgacgttc gcgctcggct gcggcgcgca attcccgatc gccgacgaag gctggggcgc
     1201 gcgccccgtg cccgtcgtca aaggctatcc cgagctcgcg gcccacatgg ttcagtcgct
     1261 cgtgctcgac gagttcgaca tgaccatcgt caacgagatg caggtcgacc acggcttgac
     1321 cgtgcccatg acgctgctgt tcgggcaacc gcaagcgtgg cccgtgcgag tgatcccgct
     1381 ggccgtcaac gtcattcaat atccggcgcc gacgggcaat cgctgctatc aactcggcaa
     1441 ggcgatcaag aaggccgtcg acacctggga cgaagatctg cgtgtcgtga tcttcggcac
     1501 gggcggcatg tcgcaccaga ttcaaggccc gcgcgccggg ctcatcaaca cggaattcga
     1561 tacggcgttt ctcgacgacc tgactcgcaa ccccgagcgg ctcgcccgca ttgcgccgct
     1621 cgagtacctg cgtgacgcgg gcgccgaagc catcgagctc gtgatgtggc tcatcatgcg
     1681 cggcgcgctc ggtccgcgcg cgcgcgaggt ctaccgcttc tatcacgtgc cggcatcgtc
     1741 gacgggcgtc ggcaacatca tcctcgaggc cgcctagcta gcggatcgct gcgtacatga
     1801 gcgcaccgct gacgatcgag cggtacacgg cccccgagag gtttctcgcc gccgcgcagg
     1861 aatggctgct cgaagccgag gtcgagaaca acctgctgct cggcatcgcg ttgaacttgc
     1921 gcggccgcga ggctcgcgag ccacggccat actggctcat cgtgcgcgcc gacgagcgaa
     1981 tcgtggcctg cgcctgccgc accccgccga acccgctcgt gctgtcgcac gcgccggcga
     2041 cggcgatcgc gcggctcgcg acagaactga gcgccgcaga tcctgcgctg agcggcgtga
     2101 ccgggcccga gacggagagt gcggcgtttg cgtccgcgtg gacggcgcag cgcggcggcc
     2161 acccgcgtac tcggtttcgc cttcgactgc atgagctgtc acacgtgacg tttgccggcg
     2221 tcgcgaaagg cgggctgcgc aaagcgacga cggccgacct tgcgctcgcg cgcgaatgga
     2281 ccgatgccta cgtccgcgac acgggcatca caccgagcgt cgacatcgca cagcgattga
     2341 tagatcgcga gcagctgttc ctgtggatcg acggccgcga gccgcgggcc atggtcgccg
     2401 cgactcgcga gacgcgctcg ggctgctcga tcaacggcgt ctatacgccg ccgcatttca
     2461 ggcgccacgg ctatgcgacg gcggccgtcg ccgcgctcag cggcgagcga ctcgcgggcg
     2521 gcttgcgctt ctgttgtctc tatacggacg ccacgaatcc gacttcgaac tcgatctacg
     2581 caaaaattgg ctatcgtccg atacgcgacg acgtcgagat cgcgttcgac gcgtagcgtc
     2641 agtgccccgg cttcagcgtg cccttctcgc gcgcgacgtc gagcaagtgc ggatagaact
     2701 gcacgaacgt gcgctcgacg gcgccgttct cggaatgaca ctggacgcac ggcgcgacat
     2761 cggcgcccgc gagcggtttg gctgcaggcg ccaaccgatt gccggggccg aatgcgtaga
     2821 acgcccagcc gtcggggaag cgcgagtcct tgacctctgc ttcgaggaac gtcagctgcg
     2881 tggcaaaccg gcccgcacga ttgatcgagc cctcggtttg cgagccccga aactcgagca
     2941 tgaacaccgt gccgtccggc caagtgccgg tgcgcatgaa gtgctggtac gaggtcggat
     3001 tgacgaacgc gtgcgtaaac cgcgggtcgt cttgcgtggc ggcctgcgtg ccgcgctcgc
     3061 cgtcgtaggt catgccgagg ccggcgccga tgaaaggcca cgagcgatag tcggccgggc
     3121 gcacgaggtc cgtgccgttt tcgtagcgcg gcccgtcgcc gctcggctgc tgtgagctcg
     3181 ctgtcattgc cgcgagcagc atcgccgccg ccgttaccat cgaaccccgc
//
LOCUS       DATLEE010000192         2368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7558905, whole genome shotgun sequence.
ACCESSION   DATLEE010000192 DATLEE010000000
VERSION     DATLEE010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7558905"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VM692_04105"
     CDS             complement(<1..1047)
                     /locus_tag="VM692_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUQ51380.1"
                     /translation="MPDSAAALERIASIVGPRGVVAHADAAPLLRDERGLYQGAAALV
                     VRPASVEECAGVVRVCHDARIGVVPQGGNTSYCGGATPFDDPWKSLEGDGERRQILLS
                     LSRLNRVREVDAIGFTMTTEAGVVLARAQEAARAANLLFPLSMGSEGSAQIGGNLATN
                     AGGLAVLRYGTMRELVLGLEVVLPNGDVLSELKGLRKDNTGYDSKALFLGSEGTLGVI
                     TAAVLKLFPEPRSRATAWLAVASIDAACALLGRARRESGDQVVSAEYVSRRSLDLVLR
                     HVDGARDPLGSPAEHYVLLELSSADADERLRATLERVLATGLADGEILDGTIAESEAQ
                     RRALWLLRERVPEAE"
     gene            complement(1108..>2368)
                     /locus_tag="VM692_04110"
     CDS             complement(1108..>2368)
                     /locus_tag="VM692_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ51381.1"
                     /translation="DTDNVESYEWELHRITLPDGQPGVVSYYFDSTRLRKAEHALREA
                     DRRKDEFLATLAHELRNPLAPVRNGLQILRMTKADATATGRVHEMLERQVGHLVRLVD
                     DLMEVARVTRGRIDLRKESIDLAAMLRSAVETSRPLIEAARHELIVDLPAEPVTLVAD
                     PIRLAQVIANLLNNAAKYTEEGGRIVLSAKREGEHAVVAVRDSGVGIPGDVLPRVFDL
                     FAQADRTYHRSQGGLGIGLTLVRTLVELHGGTVVARSEGLGHGSEFTVRLPLGLERAD
                     RRARNAGDVANVLSEHRILVVDDSSDAAESLAMLLKGLGADVHTASDGPAALEQLVTA
                     RPSVMLLDIGMPGMDGLEVARRARQLPESRELTLIALSGWGQDEDRRRSREAGIDYHL
                     VKPINLDELSHLLTALAPARAHRARVN"
BASE COUNT          357 a          818 c          814 g          379 t
ORIGIN      
        1 ctcggcttcg ggcacgcgct cgcgcagcaa ccacagcgcg cgcctctgcg cttccgactc
       61 tgcgattgtg ccgtcgagga tctcgccgtc ggcgaggccc gtggccagca cgcgctcgag
      121 cgtggcgcgt agcctctcgt cggcgtcggc cgacgagagc tcgagcagca cgtagtgctc
      181 ggccggcgag ccgagcggat cgcgcgcgcc gtcgacgtgg cgcagaacga ggtcgagcga
      241 gcggcgcgag acgtactcgg ccgacacgac ctgatcgccg ctctcgcgac gcgcgcggcc
      301 gagcagcgca caagccgcat cgatgcttgc gacggcgagc cacgccgtcg cgcgcgaccg
      361 cggctcggga aagagcttca gcacggccgc cgtgatgacg ccgagcgtac cttcgctgcc
      421 gaggaacaag gctttcgagt cgtaacccgt gttgtccttg cgtaagccct tcagctccga
      481 cagcacgtcg ccgttcggca gtacgacttc gaggccgagc acgagctcgc gcatcgttcc
      541 atagcgcagc acggccaggc cgccggcgtt ggtcgcgaga ttgccgccga tctgcgccga
      601 gccttcggat cccatgctga gcggaaacag caggtttgcc gcgcgcgccg cttcttgcgc
      661 acgcgcgagc actacgccgg cctcggtcgt catcgtgaag ccgattgcgt cgacctcgcg
      721 cacgcggttc aagcgcgaca atgacagcag gatctgccgg cgttcgccgt ccccctccag
      781 ggacttccaa gggtcgtcga acggcgtcgc accgccgcag tagctcgtgt tgccgccctg
      841 cggcacgacg ccgatgcgcg cgtcgtggca cacgcgcacg acacccgcgc attcttcgac
      901 tgaggccggc cgcacgacga gcgcggcggc gccttgatag agcccgcgct cgtctcgcag
      961 cagcggcgcg gcgtcggcgt gtgccaccac gccgcgcgga cctacgatcg atgcgatacg
     1021 ttcgagcgct gcggccgaat cgggcatgga gctcagcggg tcgactaagt gcgcccggat
     1081 attacaccga ctgggcgcgg agtcgtttca gttgacgcgc gcgcgatggg cgcgcgccgg
     1141 cgcgagcgcc gtcagcaggt ggctgagctc gtcgagattg atcggcttga cgaggtgata
     1201 gtcgatgccg gcttcacgcg agcggcggcg atcttcgtcc tggccccaac cggacagcgc
     1261 gatcagcgtc agctcgcggc tttccggtag ttgccgcgcg cggcgcgcga cctcgaggcc
     1321 gtccatgccc ggcatgccga tgtcgagcag catcacggag ggccgagccg tcacgagctg
     1381 ctcgagcgcc gctgggccgt cgctcgccgt gtgcacgtcg gcgccgaggc ctttgagcag
     1441 catggccaga ctctcggcgg catcgctgct gtcgtcgacg acgagtatgc gatgctccga
     1501 taacacgttg gcgacgtcgc cggcattacg cgctcgcctg tcggcgcgct ccagtcccag
     1561 tggcaagcgg accgtgaact cgctgccgtg accgaggccc tcgctgcgcg cgactacggt
     1621 gccgccgtgc agctcgacga gggtacggac gagcgtgagg ccgatgccga gcccgccctg
     1681 cgagcggtga taggtgcgat ctgcttgcgc gaacaggtcg aacacgcgcg gcaggacgtc
     1741 gccgggaatg ccgacgccgc tgtcgcgcac ggcgacgact gcatgttcgc cctcgcgctt
     1801 cgccgacaga acgatacggc cgccctcctc ggtatatttc gccgcgttgt tcaacaggtt
     1861 cgcgatgacc tgcgcgagcc gtatcgggtc ggccaccaac gtcacaggct cggccggcaa
     1921 gtcgacgatg agctcgtggc gcgccgcttc gatcagcggc cggctcgttt caacggcact
     1981 gcgcagcatc gctgccaagt cgatggattc tttgcgcagg tcgatccggc cgcgcgtgac
     2041 gcgcgcgacc tccatgaggt cgtcgacgag ccgcacgaga tggccgacct gtcgctcgag
     2101 catctcatgc acacggccgg tggctgtcgc gtcggccttc gtcatgcgga ggatctgcaa
     2161 gccgttgcgg accggcgcga gcgggttgcg cagctcgtgc gccaacgtcg cgagaaattc
     2221 gtccttgcgc cgatcggcct cgcgcagcgc gtgctcggcc ttgcgaagcc gcgtcgagtc
     2281 gaaatagtag gagacaacgc ccggttgccc gtccggcagc gtgatgcggt gcagctccca
     2341 ttcgtacgac tcgacgttgt cggtgtcg
//
LOCUS       DATLEE010000193         2532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9797315, whole genome shotgun sequence.
ACCESSION   DATLEE010000193 DATLEE010000000
VERSION     DATLEE010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9797315"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1073
                     /locus_tag="VM692_04115"
     CDS             288..1073
                     /locus_tag="VM692_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51382.1"
                     /translation="MIMKKLAFATLVAMGMTTAAIAQQPSSQQPGQRSTQPSTQQPGQ
                     SRSQQPGSSSQSTTPRGGDRTEQVSFETADKNGDGRVNREEASTIDGFDFSRADTNSD
                     AALSRQEFMAAMATSTPRDGGAGSASSRSGQTGASSSSQGSASARSGQSGSQSGSSAQ
                     SGGSARPGATAQSQGGSRDQSGGARDQGGSRSGEQGAGSSRESATAQVTFDTADKNKD
                     GKVSREEASDIRGLNFSSADVNDDASLSRQEFQTAMAGIQPRG"
     gene            complement(1146..1859)
                     /locus_tag="VM692_04120"
     CDS             complement(1146..1859)
                     /locus_tag="VM692_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HUQ51383.1"
                     /translation="MAKLKLTVLSLRYSSWSMRPWLTLYHTGAPFETETVALPHMARQ
                     SETTSLGDRRKLGSVRGLFPVLRVDGTPIHESLAICEYIADAFPDAQLWPAESLQRAA
                     ARAICSEMVSGFTTMRNELSCHLFGRVPSFTPTPAALADVERVFEIWNEKLDASGGPF
                     LFGRFSIADSMYFPVLTRFRTYGVKIPSTLVPYAEALEAHPAVLALMAVASSAPRIPV
                     YDDYLRHCGGDPDAALRAK"
     gene            complement(1916..2203)
                     /locus_tag="VM692_04125"
     CDS             complement(1916..2203)
                     /locus_tag="VM692_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HUQ51384.1"
                     /translation="MDLKMFATVFGTVFLAELGDKTQLATLLFASRGTATLWTIFFAA
                     SAALVLTSAIGVAAGALVSHYLNPKYLSYAAGVGFIAIGIWTLVQAGTSAS"
     gene            complement(2208..>2532)
                     /locus_tag="VM692_04130"
     CDS             complement(2208..>2532)
                     /locus_tag="VM692_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HUQ51385.1"
                     /translation="NGDVTVKTPWLEIDDVFYEARGTAWALLVFLRAAQFDFERTLED
                     KNAVVSLRQIIRELERGLAPIRSPVILNGQEYGLFPNHSLVLASYLSRAHSAVADLRR
                     LLEQG"
BASE COUNT          503 a          819 c          839 g          371 t
ORIGIN      
        1 ggaaattaac tcgcgagtga tgcgccaccg cggccggcgc cgtgagcact gcaacgatga
       61 aggtcgtgag gcgtgccatg gaatcctctt cggcgggcca aaaccccgca cagcaactgc
      121 cggggactat atctgagaaa acctttccca agctgcgaaa gatccgtgcg tgacggtccc
      181 gctgtctcgc gagctgagtg gcacatgtct tgcagcctcc gccggcctgc ttgccgcaag
      241 agcgcggtgg gggtttctcg ttttttaacc actaggaagg tgcagtcatg atcatgaaga
      301 aactagcatt cgcaaccttg gtcgcgatgg gcatgaccac cgctgcaatc gcgcagcagc
      361 catcgtcgca acaaccgggc cagcgttcga cgcagccgtc gacgcaacag ccgggccaat
      421 cccgcagtca gcagccgggc agcagcagtc aatcgacgac gccgcgcggc ggcgaccgaa
      481 ccgagcaggt cagcttcgag accgccgaca agaacggtga cggccgcgtc aatcgcgagg
      541 aagcgagcac catcgacggg ttcgactttt cgcgtgcgga cacgaacagc gacgccgctc
      601 tgagtcgcca agaattcatg gcggcgatgg cgacgtccac gccgcgcgac ggcggtgccg
      661 gctcggcaag ctcgcgcagc ggccaaacgg gggccagctc gagcagccaa ggctcggcga
      721 gcgcgcgcag cggtcagtca ggctcgcagt cgggctcgag cgcccaatcg ggcggcagcg
      781 cacggcccgg cgcgaccgcg cagagccaag gcggctcgcg cgatcagtcc ggtggcgcgc
      841 gtgaccaagg cggctcacgc agcggtgagc aaggtgccgg cagctcacgc gaaagcgcta
      901 cggcgcaggt gactttcgac acggccgaca aaaacaagga cggcaaggtc agccgtgaag
      961 aggcgagcga cattcgaggc ctgaacttct cgtcggcgga cgtcaacgac gacgcgtcgt
     1021 tgagccgtca ggagttccag accgcgatgg caggcatcca gccgcgaggc taatgcctac
     1081 ggcgcatgcg accggcatgc gctgacgtcg agccaacgga agaggccgcg cttgcgcggc
     1141 ctctttcatt ttgcgcgcaa cgctgcgtcg ggatcgccgc cgcagtggcg caaataatcg
     1201 tcgtagaccg gaattcgcgg cgcggagctc gcgaccgcca tcagtgccag caccgcggga
     1261 tgcgcttcga gcgcctcggc atacggcacg agcgtgctcg gaatcttgac gccgtaggtg
     1321 cggaagcgcg tcagcacggg aaagtacatc gagtccgcga tcgagaaccg gccgaacaga
     1381 aagggcccgc ccgaagcgtc gagcttctcg ttccagatct cgaagacgcg ttcgacgtcg
     1441 gcgagagctg ccggcgtcgg cgtgaacgac ggcacgcggc cgaacaagtg gcacgacagc
     1501 tcgttgcgca tggtagtgaa gccgctaacc atctcgctgc agatcgcgcg cgccgctgca
     1561 cgctgcagcg attccgccgg ccagagctga gcgtcgggaa acgcatcggc gatgtattcg
     1621 cagattgcga gcgactcgtg gatcggagtg ccgtcgacgc gcagcacggg gaacagcccg
     1681 cgtacgctgc cgagcttccg ccgatcgccg agcgacgtcg tctcgctttg ccgcgccatg
     1741 tgcggcaggg cgacggtttc cgtctcgaac ggcgcgccgg tgtgatacag cgtgagccac
     1801 ggtcgcatcg accacgacga gtaccgcagg ctcaatacgg tcagcttgag tttcgccatg
     1861 gacggaagat ggcccggcgt gccggtcgga gtcaatccgg ggcgctgttc gtaacctagc
     1921 tcgccgaagt tccggcttgc acgagcgtcc agatgccgat cgcaatgaag ccgaccccgg
     1981 ccgcatacga caggtacttc gggttcaaat agtgcgacac gagcgcgccg gcggccacgc
     2041 cgatcgccga ggtcagcacg agcgccgccg acgcggcgaa aaagatcgtc cacaatgtcg
     2101 cggtgccgcg cgacgcaaac agcagcgtcg cgagctgcgt cttgtcgccg agctcggcca
     2161 ggaacaccgt gccgaacacc gtcgcgaaca tctttaagtc catgccgcta accctgctcc
     2221 aacaagcggc gcaggtcggc gacagccgaa tgcgcgcgcg acagatacga tgcgagcacg
     2281 agcgaatgat tcggaaacag tccgtactcc tgaccgttca agatcacggg gctacggatc
     2341 ggcgccaagc ccctctcgag ctcgcggatg atttgcctga ggctcacgac ggcgttcttg
     2401 tcctcgagcg tgcgctcgaa gtcgaactgc gcggcgcgta ggaacacgag cagcgcccac
     2461 gccgtgccgc gcgcctcgta gaagacgtcg tcgatctcga gccacggcgt tttgacggtg
     2521 acgtcgccgt tc
//
LOCUS       DATLEE010000194         6587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_216073, whole genome shotgun sequence.
ACCESSION   DATLEE010000194 DATLEE010000000
VERSION     DATLEE010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216073"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VM692_04135"
     CDS             <1..253
                     /locus_tag="VM692_04135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUQ51386.1"
                     /translation="IVLLGGADKQVADEVAQRIRNVVFSTTLEVDVKMVRIKVNVGAA
                     SFPDSGNNLQAVMAAADRAMYKDKDLREPPKGKLIIQKR"
     gene            282..503
                     /locus_tag="VM692_04140"
     CDS             282..503
                     /locus_tag="VM692_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51387.1"
                     /translation="MNDTTKGPPQKPGFPLWGSIAIPAGLLWGTALGAGAGLLFGNLM
                     IGALIGAGLGVGVGFTLFAAAIVIASSKL"
     gene            513..866
                     /locus_tag="VM692_04145"
     CDS             513..866
                     /locus_tag="VM692_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51388.1"
                     /translation="MTKAINWLGRALGLVCAASLAGIWAFVLWVPAAGLTVTGISVVT
                     ALLLVAFAVFAAIAAVYGHAAVIALLFLASFFPVGAFLMPTDHWLKSVGWIDLGLLVA
                     AVLIWATRRREAATA"
     gene            933..1100
                     /locus_tag="VM692_04150"
     CDS             933..1100
                     /locus_tag="VM692_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3012 domain-containing protein"
                     /protein_id="HUQ51389.1"
                     /translation="MRRVWQVAVLASLTTWLVACAPEVGSTRWCEAMRDKARGDWTAN
                     EALDFARSCVL"
     gene            1111..1578
                     /locus_tag="VM692_04155"
     CDS             1111..1578
                     /locus_tag="VM692_04155"
                     /inference="COORDINATES: protein motif:HMM:NF040341.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HUQ51390.1"
                     /translation="MRSITTLRAALASALALAATSAVADAELDAARARWQSAGLAGYE
                     YGYHKYCECHRDSPPETVVTVRGGKVVGVRHRPVGSTTEVPAADKNLEYYWTVDGLFG
                     LISSAQQRGVQVRAQYDAALGFPREVYIDYDANFIGDELDVRLTGVTAVAATR"
     gene            1727..1981
                     /locus_tag="VM692_04160"
     CDS             1727..1981
                     /locus_tag="VM692_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HUQ51391.1"
                     /translation="MNIYVGNLSYGMSEDELRNAFGEYGEVSSVKILMDRETGRSRGF
                     GFVEMPNNSEAEKAIAQLNGKDVGGRALRINEARPREQHR"
     gene            2230..2496
                     /locus_tag="VM692_04165"
     CDS             2230..2496
                     /locus_tag="VM692_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51392.1"
                     /translation="MFYLKWTPSELATLRAGALYTEVDEDGWVQRELGIGDDGLITHQ
                     LVPCNARPGWFGLARLSFAMLDSNVTKAEFESLWHAGAARSAEP"
     gene            2637..3041
                     /locus_tag="VM692_04170"
     CDS             2637..3041
                     /locus_tag="VM692_04170"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HUQ51393.1"
                     /translation="MTAFVGAAIALQAGAQQSFKVRLRPVPIEASTAANTIGAGEATA
                     QLAGTKLTVRGNFAGLKGSATVAQLHQGPVMGVRGPAIADLTVPSATSGNFSAEVTLT
                     AAQVEGLRQGHVYLQIHSETAPDGNLWGWLLP"
     gene            3038..5170
                     /locus_tag="VM692_04175"
     CDS             3038..5170
                     /locus_tag="VM692_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51394.1"
                     /translation="MSARSGRGVLAFAAAALASAAQAQTGPFTEGQAAAGRSSYVANC
                     AGCHQPDMRGSNEARPLVGPDFMRTWSARTTQELIAFLGVTMPPPPAAPGSLGPQSYV
                     NLAAFLLQANGATAGTSELSAATTVAIGSVANGTMADSVRAAIASTVPAGAASAAGRT
                     GISVAGQIEGFRPVTDAMLRRLPADDWLMIRGNYQAWNFSELNQITRDNVDDLQLEWI
                     WAMTDGGWNEPAPVVHDGVLYLNNMGNIIQALDAATGELIWENRVGPLTQGGSAMRGL
                     ALYGDKLFVATSDARLVALAARNGQTVWETVIGDRTEGSFQISSGPIVIGGKVLQGMG
                     GCTLFRKEKCFISAYDADNGRQLWKFATVATTGTPGGDTWGDVQDVFRAGGDTWITGS
                     YDPDLDTTYWGVAQAKPWVPASRGNSALDRALYTSSTLALNPNDGTLKWYYQHAPGEA
                     LDLDEVYERVLIDVDGEKTLFTVGKPGILWKLDRENGRFIDHKETVFQNVFDRIDPET
                     GQPRYRADIVAGKVGEWVQGCPSTEGGHNWQAMSHHAPSSRLIIPLSQSCIEIRGQQV
                     EQVAGGGSSGADRRFFEMPGSDGNVGKLAAYDTRTMQELWSLQQRAPFLTAVLSTRGG
                     VAFVGDLDRKFKAVDVATGKVLWETRLTTSVQGFPITFTAGGRQYVAVTTGVGGGSPR
                     VVPSLITPEIHHPANGNALYVFSLPARD"
     gene            5217..5292
                     /locus_tag="VM692_04180"
     tRNA            5217..5292
                     /locus_tag="VM692_04180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5250..5252,aa:Arg,seq:ccg)
     gene            complement(5294..5809)
                     /locus_tag="VM692_04185"
     CDS             complement(5294..5809)
                     /locus_tag="VM692_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUQ51395.1"
                     /translation="MRQHDQQFFDSFMLVIGILMGVAVGLFFLVRAISIDTQGRYITE
                     DPTVQEAIEERIKPVGRVLLLGDAELAAAAAAAVKAPTPVSTPLTGPQVYNAACIVCH
                     QPGVATGAPTIGDTAAWAPRIAQGIDTLYMHALMGYQGEKGYMPPKGGRVDLSDDEIK
                     GAVDYLVEQGK"
     gene            complement(5962..>6587)
                     /locus_tag="VM692_04190"
     CDS             complement(5962..>6587)
                     /locus_tag="VM692_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HUQ51396.1"
                     /translation="ETHRVRACWFMTSFQNPLGATMPDDKKRDLVRLLRSRGVPLIED
                     DVYAELYFGARRPPPAKAFDDRGLVLHCGSLSKSLAPGYRIGWVAAGQLARDVQRRKI
                     MTSRSTSIPVQEGIAQALKGGGYDAHLKSLRRALAAQQTSMLDSLRKHWRGHYRVTRP
                     EGGNSCGSSYQTKSTLSTSTAVPWSTGSASHQDPSFRRAVSSETLCV"
BASE COUNT         1113 a         2141 c         2253 g         1080 t
ORIGIN      
        1 catcgtgctg ctcggcggcg cggacaaaca ggtggctgac gaggtcgcgc aacgcatccg
       61 caatgtcgtg ttctcgacga cgctcgaggt cgacgtgaag atggtgcgca tcaaggtcaa
      121 cgtcggcgcc gcgtcgttcc ccgacagcgg caacaatctt caggccgtga tggcagcggc
      181 cgatcgagcc atgtacaagg acaaagacct gcgcgagccg ccgaagggca agctgatcat
      241 tcagaagcgc tgacgcgcgc cgccagcgct cggggtgcgc cgtgaacgac acgaccaagg
      301 gaccgccgca aaagcccggg ttcccgcttt ggggctcgat cgccataccc gcgggcttgt
      361 tgtggggcac ggcgcttggc gcgggcgccg gactgctgtt cggcaatctc atgatcggtg
      421 cattgatcgg tgccgggctc ggtgtcggcg tcggctttac cctgttcgcc gctgccatcg
      481 tgattgcgtc gagcaagctt tagggggagc ggatgaccaa ggcgatcaac tggctcgggc
      541 gagcgctcgg cctcgtgtgc gcggcaagtc tcgcgggaat ctgggcgttc gtgctctggg
      601 tgccggccgc gggcctgacc gtcactggca tcagcgtcgt gacggcgttg ctgctcgtgg
      661 cgttcgccgt gttcgcggcc atcgcggctg tctacggcca cgcggccgtg atcgcgttgc
      721 tgtttttggc gtcgttcttt cccgtcggcg cgttcttgat gccgaccgac cactggctca
      781 agtcggtcgg ctggatcgat ctcgggctgc tcgtcgccgc cgtgctgatt tgggccacca
      841 ggcggcgcga ggccgccacg gcctagcgtt gccgagacgt gcgttgctct agaatggccg
      901 ccgtttcgag cgacgaagca atggagcgaa cgatgcgcag agtttggcaa gtagcggtgc
      961 tcgcgagtct gacgacatgg cttgtcgcgt gcgcgcccga agtcggctcg acgcgctggt
     1021 gcgaggcgat gcgggataag gcgcgcggcg actggacggc gaacgaagcg ctcgacttcg
     1081 cgcgcagctg cgtgctgtag agtgggtgcc atgcgctcga tcacgacact gcgcgcggca
     1141 cttgccagcg cgctcgcgct cgccgcgacc tcagccgtcg ccgacgccga gctcgacgcg
     1201 gcgcgtgcgc gctggcagtc ggccggtctc gccggctacg agtacggcta tcacaaatac
     1261 tgcgagtgcc accgcgactc gccgccggag acggtcgtca cggtgcgcgg cggcaaggtc
     1321 gtcggcgtgc ggcatcggcc cgtcggcagc acgaccgaag taccggcggc cgataagaac
     1381 ctcgagtact actggactgt ggacgggctg ttcggactga tctcgtcggc gcaacagcgc
     1441 ggagtgcagg tgcgcgcgca atacgacgcc gcgctcggct ttccgcgcga ggtctacatc
     1501 gactacgacg ccaacttcat cggcgacgag ttggacgttc gcctaacggg cgtaacggcc
     1561 gtggccgcca cccgttagag ccttcgcaag aagcccgcta agtcgggagt atctcgggat
     1621 tgaccgcaca agaggggcgg ccgtagagtt ttcacggcgg cgctcgatct cacgtcgttg
     1681 gttgagtgcc tttcggggga cgggaaacga cgtgagagcc tcggtgatga atatctacgt
     1741 cggtaatctg tcctatggca tgagcgaaga cgagcttcgc aatgcgttcg gtgagtatgg
     1801 cgaggtgtcg tccgtcaaga tcctcatgga tcgtgagacg ggccgctcgc gcggtttcgg
     1861 cttcgtcgag atgccgaaca acagcgaagc cgaaaaggcg attgcacagc tcaacggtaa
     1921 ggacgtcggc ggacgcgcgc tgcgcatcaa cgaagcgcgg ccgcgagagc agcaccgcta
     1981 gccgccgttc accgtgacgt cgagtggcgg ctcgacgaag cggaactgca cccgcgcggc
     2041 caacggttcg ggccgtgtgc agaatcagcg cggagctcat ccgcgtccgt cgtgctacac
     2101 cccgacgtgg cgttatgttc cgttgcagcg gccggcgttg tcgccccgct ataatctaat
     2161 cgccaagctg acaacaacaa cgaccggccg cgcccacggg cgccccggcg acacagggac
     2221 ggaacagaag tgttttatct gaaatggacc ccgagcgagc tcgccacact acgcgccggc
     2281 gctttgtata ccgaggtgga cgaggacggc tgggtgcagc gcgagctcgg catcggcgac
     2341 gacgggctca tcacgcatca gctcgtgccc tgtaacgcgc ggccgggttg gttcggtctc
     2401 gcacgcttgt cgttcgcgat gctggacagc aacgtcacga aggccgaatt cgaatccttg
     2461 tggcacgcgg gagccgcacg gtccgccgag ccctgatcgt cggcgtgtcg gcccctcgct
     2521 agccgcgccg gtgccgccgt ctgtagaatc gcggcaagaa caacgacgag ccaccggggg
     2581 gtgccatggc ttcgccggct cattcgcgac gcaacgcgcg tcgattccat tggtgcttga
     2641 cggctttcgt cggcgccgcg atcgcgctgc aagcgggcgc acagcaaagc ttcaaggttc
     2701 gtctgcgccc agtgccgatc gaagcctcga cggccgccaa cacgatcggc gccggcgaag
     2761 cgactgcgca actggccggc acgaagctga ccgtccgcgg caatttcgcc ggcttgaaag
     2821 gctcggccac tgtcgcgcag ctgcaccaag gccccgtcat gggtgtgcgc gggcccgcga
     2881 ttgccgatct cacggtgccc tccgcaacca gcggcaactt cagtgccgag gtcacgctga
     2941 ccgccgcgca ggtcgaaggc ctgcggcaag gccacgtcta tctgcaaatc cacagtgaga
     3001 cggctccgga cggcaacctc tggggctggt tgctgccatg agcgctcgta gcgggcgcgg
     3061 cgtcctcgca tttgcggccg cggcgctggc gagcgccgca caggctcaaa cgggcccgtt
     3121 caccgagggg caagccgcag cggggcgttc gtcgtacgtc gccaactgtg ccggctgcca
     3181 ccaacccgac atgcgcggct ccaacgaagc gcggccgctc gtcggcccgg acttcatgcg
     3241 cacctggagc gctcgcacca cccaagagct catcgcgttc ctgggcgtaa ccatgccgcc
     3301 gccgccggcc gcgccgggca gtctcgggcc gcaaagttac gtcaatctgg ccgcgttcct
     3361 gctgcaggcg aacggcgcga cggcgggcac gagcgagctc agcgccgcga cgaccgtcgc
     3421 gatcggcagc gtcgcgaacg gcacgatggc tgactccgtg cgcgcggcca ttgcgagcac
     3481 ggtccctgcc ggtgcagcga gcgctgcggg gcgcacgggc atcagcgtgg ccggccaaat
     3541 cgaggggttc cggcccgtca ccgacgccat gctgcgccgc ttgcccgccg acgactggtt
     3601 gatgatccgc ggcaactacc aggcgtggaa tttcagcgag ctcaatcaga tcacgcgcga
     3661 caacgtcgac gatctgcagc tcgagtggat ctgggcgatg acggacggcg gctggaacga
     3721 gcccgcgccc gtcgtgcacg acggcgtcct gtatctcaac aacatgggca acatcatcca
     3781 ggcgctcgac gccgcgacgg gtgagctcat ctgggagaac cgcgtcgggc cgctcacgca
     3841 gggcggctcg gccatgcgcg ggctcgcgct ctacggcgac aagctgttcg tcgcgacgag
     3901 cgatgcgcga ctcgtggcgt tggcggcgcg taacggccaa accgtttggg agaccgtgat
     3961 cggtgatcgc accgagggca gcttccaaat ttcgagcggc cccatcgtca tcggcggcaa
     4021 ggtgctgcaa gggatgggcg gctgcacgct gttccgtaag gagaagtgct tcatcagcgc
     4081 ctacgacgcc gacaacggcc ggcagttgtg gaagttcgcg accgttgcta cgacgggcac
     4141 gccgggcggc gacacgtggg gcgacgtgca ggatgtgttt cgcgccggcg gcgacacgtg
     4201 gatcacgggc agctacgacc ccgatctcga cacgacgtat tggggcgtcg cgcaagcgaa
     4261 gccctgggtg cccgcgagtc gcggcaactc ggcgctcgat cgcgcgctct acacgagctc
     4321 gacgctcgcg ctgaatccga acgacggcac attgaagtgg tattaccagc atgcgcccgg
     4381 cgaggcgctc gacctcgacg aggtttacga gcgcgtgctg atcgacgtcg acggtgagaa
     4441 gacgctgttc acggtcggca agcccggcat cctctggaaa ctcgaccgcg agaacggccg
     4501 cttcatcgat cacaaggaga cggtgttcca gaacgtcttc gaccgcatcg atcccgagac
     4561 cggccagccg cgctaccgtg ccgacatcgt cgcaggcaag gtcggcgagt gggtgcaggg
     4621 atgtccctcc accgaaggcg gccacaactg gcaggcgatg tcacaccatg cgccgtcgtc
     4681 gcggctgatc atcccgttga gccagagctg catcgagatc cgcgggcaac aagtcgagca
     4741 agtcgcgggc ggcggcagct cgggcgcgga caggcggttc ttcgagatgc cgggcagcga
     4801 cggcaacgtc ggcaagctcg ccgcgtacga cacacgcacg atgcaagagc tctggtcgct
     4861 gcagcagcgc gcgccgtttc tgacggccgt gctgtcgacg cgcggcggcg ttgcgttcgt
     4921 cggcgacctc gatcgcaagt tcaaggccgt cgacgtcgcg accggcaaag tgctgtggga
     4981 aacacggctc acgacttccg tgcagggctt tccgatcacg ttcacggccg gcggccgcca
     5041 atacgtcgcc gtgacgaccg gcgtcggcgg cggcagcccg cgcgtcgtgc cgtcgctgat
     5101 cacgccggag atccaccacc ccgcgaacgg caacgcgttg tacgtgttct ctctgccggc
     5161 gcgcgactag caacgcgagt cggctcgacg ttcggggcgg gctatactgc cgccacgcgc
     5221 ccgtagctca gtggatagag cattgccctc cggaggcaag ggtcgggggt tcgaatccct
     5281 ccgggcgcgc cagctacttg ccctgctcga cgagatagtc gacggcgcct ttgatctcgt
     5341 cgtcagacag atcgacgcgg ccgcccttcg gtggcatgta acctttctcg ccttggtagc
     5401 ccatcagcgc gtgcatgtat agcgtgtcga tgccttgcgc gatgcgcggc gcccatgccg
     5461 ccgtgtcgcc gatcgtcggc gcgcccgtgg cgacgccggg ctgatggcac acgatgcagg
     5521 cggcgttata aacctgtggg cccgtcaacg gtgtcgagac gggtgtcgga gccttgacgg
     5581 cagcggccgc cgcggccgcg agctcggcat cgcctagcag cagcacgcgg ccaacgggct
     5641 tgatgcgctc ctcgatggct tcctgcaccg tcgggtcttc ggtgatgtag cggccttgcg
     5701 tatcgatcga gatcgcgcgc acgaggaaga acaagcccac ggccacgccc atgaggatgc
     5761 cgatgacgag catgaagctg tcgaagaact gctgatcgtg ctggcgcaac gccgtgctcc
     5821 cgagcctttg aatccggcgg cgattatagc cgacgcaagc gccgaatcga ggcgaaccgg
     5881 cgcttcatca ccgcagatcg gctagcttgg cgagccgctg cacggcgcga tcgagctcgt
     5941 cggtccaggg atgtccgtag ttcaaacgca cagagtttcg gaactcacgg cgcggcgaaa
     6001 agatgggtcc tggtgcgatg ctgatcccgt gctccagggc acggcggtgg aggtcgagag
     6061 agtcgacttc gtttggtagc tcgagccaca cgagttaccg ccctcggggc gagtcacccg
     6121 gtaatggccg cgccagtgct tgcgcagcga atcgagcatc gacgtctgct gcgccgccag
     6181 cgcgcggcgc aggctcttca ggtgcgcgtc gtagccaccg cctttcaacg cttgcgcaat
     6241 accttcttgc acgggaatgc tcgtcgaccg cgaggtcatg atcttgcgac gttgcacgtc
     6301 gcgcgcgagc tgcccggccg caacccagcc gatgcggtag ccgggcgcca gcgacttcga
     6361 caacgagccg cagtgcagca cgagcccgcg gtcgtcgaat gccttcgcgg gcggaggccg
     6421 ccgggcgccg aagtacagct ccgcgtagac gtcgtcctcg atcagaggca cgcctcgcga
     6481 tcgaagcagc cgcaccaagt cgcgcttctt gtcgtcgggc atcgtcgcgc caagggggtt
     6541 ctggaacgac gtcatgaacc aacaggcgcg cacgcgatgc gtctcga
//
LOCUS       DATLEE010000195         9715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_962020, whole genome shotgun sequence.
ACCESSION   DATLEE010000195 DATLEE010000000
VERSION     DATLEE010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962020"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /gene="tatC"
                     /locus_tag="VM692_04195"
     CDS             <1..553
                     /gene="tatC"
                     /locus_tag="VM692_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HUQ51397.1"
                     /translation="DVAAPFLTPMKTAVFVAVFIAMPVVLFQIWQFVAPGLYRREKRL
                     AIPLMISSIVLFYSGVAFAYFIVFKVMFGFFAGVTPSGVNMTPDISAYLSFVLGIFLS
                     FGLAFEVPIATVLLLWSSLVSMKALKSARPYVFLGAFVAGMLLAPDVFSMTLMAIPMY
                     ALYEGGLLMARFLLPIEKTAVQT"
     gene            556..1704
                     /locus_tag="VM692_04200"
     CDS             556..1704
                     /locus_tag="VM692_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HUQ51398.1"
                     /translation="MKPIDSKLPTVGTTIFTLMSQLAQQHRAVNLSQGFPDFRPPAAL
                     LELVERCLRGQYHQYALMPGSMALRTAIAAKLAAMYDVVADPVTEITVTSGATEAIFG
                     AIQALVRRDDDVIVFDPCYDSYEPSIALAGGRAVHLPLTQPDFAIDWDRLRSALSSRT
                     RLVIVNSPHNPSGALLTRADLDRLAEALRPYDCWLLSDEVYEHVVFDGCRHATVLAQP
                     ELAQRCFAVFSFGKTYHATGWKLGYCVAPPALTAELRRVHQYVTFASTSPLQQALAEY
                     MTQHPEHHVGLPAFYQERRDYFAQLLAATKFRPRRAAGTYFQLADYSAISTLPDVEFA
                     RWLTTEHGVATIPISVFYRDPPDTKLVRFCFAKVNATLDAAAERLRRL"
     gene            complement(1713..3689)
                     /locus_tag="VM692_04205"
     CDS             complement(1713..3689)
                     /locus_tag="VM692_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HUQ51399.1"
                     /translation="MSAQTSAPPQLTARAIVLAVVLAMVLAAANTYLGLFAGMTIASA
                     IPAAVVSMAVLRVLGGGGILENNIVQTGGSAGASLASGVIFTIPALVILGFWDDFDFL
                     WVVAIAALGGLLGVLFSVPLRRSLIVDQGLTFPEGKAAAEVLKAGENPSQGVKILGGA
                     ALGGGALKLAAASGLGLIPDTAAIAGYVGKGIAYFGTNLSPALLGVGYIVGLNIGAVV
                     LLGGVISWNVAIPLYAAFGLDGNPDLANAVAGLDAEAAAGVLWSRQIRYLGVGAMLVG
                     GLWALFSLRTSLVSGIKSGLTAARAGAQTRVAHTERDLPMKGVLIGIVLFTLPLLALY
                     QSIVGSFAVSVPMTIIMIVAGFLFCSVSGYMAGLVGTSNNPVSGITIGTILFSAGVLL
                     LLMGPRSTIGPVAAIMIGAVVCCAACIAGDNLQDLKCGHIVGATPWRQQVMLAIGACA
                     SALVMAPVLNLLAHAYGIGAPTDAHPQALLAPQATLMASVAQGLFGGSLPWPTIGVGA
                     AIGAFIIIIDELAKAKRATFRVPVLAAAVGIYLPLDLTVPIFVGGLLAWFCERTLKSS
                     VGISSPEEAERLGRKGMLFAAGVITGEALMGIAIAVPIWLTERADVLALPLDYRLGQS
                     TAGEGLGLAALALLAYWLYRTATSKSVDTTPSPR"
     gene            complement(3694..5229)
                     /locus_tag="VM692_04210"
     CDS             complement(3694..5229)
                     /locus_tag="VM692_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HUQ51400.1"
                     /translation="MSSTATTGSAHASAASREFLGHPRGLATLFMTEFFERFTYYGMR
                     ALLVLFLVAATSAANPGFGMDREVAGAVYGLYTGAVYLFGLPGGWMADRLIGQRNAIF
                     WGGIIIAAGNFILALPASPAVFYVGLAVIVIGVGLLKPNISAIVGSLYEGQPGARRDA
                     GFSIFYMGINLGALIAPLIAGTIGEGWNWRGGFFCAGLFMCLGVVQYKLTEHYLGDAG
                     RLPPGVSAAEQRRGWLWIAVGSTLLVAAAGLMFFGVIPVDVTTLAQVAGTAMVAIAFA
                     FFGYVLFFAGLSREERLRVGVIAVFFLCSAVFWAGFEQQATTFNLFAVDYTDRSLLGG
                     LFPEGVHPASWYQSVNPVCIVLFAPFFAWLWVALGKRNLDPSAPLKFGFGLLLLGLGF
                     LVMVLAADVVLATGNDVAPTWLLMAYLLHTFGELCLAPVGLSNITKLAPPRFAGQMMG
                     TWFLGLAVGNLLAGMIGGEISAADVSELPSKLMTMTLVGAAAGLLMMIFARYIRGWMG
                     GIR"
     gene            complement(5339..6073)
                     /locus_tag="VM692_04215"
     CDS             complement(5339..6073)
                     /locus_tag="VM692_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HUQ51401.1"
                     /translation="MDVKKLWLLATFALGACGGPAEPVGETDPAVAPTAQSQDPLEPE
                     LVASDPSGAPTTGRFEMGTHYMRLSPTQPTSSNPDQVEVAEVFWYGCPHCFAFDPLLE
                     QWRAQKPDYVNFLRIPAVWNPLLQLHARAFYTAEALGKGAQMHAEFFREIHERGNRLD
                     SEEKLQEFFGRFNVDAAAFKTAFDSFAVQEKLQRADELNRRYRIGSVPTIIVNGKYTT
                     DGPAAGSYEELLALIGELVASERAGR"
     gene            complement(6073..6729)
                     /locus_tag="VM692_04220"
     CDS             complement(6073..6729)
                     /locus_tag="VM692_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUQ51402.1"
                     /translation="MTTLTRTRFGLLLTALLGGSLAAMSVAAQEGAAAAAEGSVEAGR
                     TKSVTCAACHGADGNSLTPDWPMLAGQHANYIVRQLKAFKNGERTDVTMKPFADMLSE
                     QDMLDVAAYFASQTPTPKGADPTLVGLGQQIYRGGVPARGVAACIACHGPEGNGNPLA
                     AYPRVGGQHAAYVTKQLNLYASGDRRSDVDVNQMMRNVAGQLFEDEIRALASYVQGLD
                     "
     gene            6816..7400
                     /gene="yihA"
                     /locus_tag="VM692_04225"
     CDS             6816..7400
                     /gene="yihA"
                     /locus_tag="VM692_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HUQ51403.1"
                     /translation="MPTAIFLKSAAIPNDFPADEGREVAFVGRSNSGKSTAVNILTGT
                     KKLARVSKTPGRTQLLNFFSFGEGRRLVDLPGYGFARVAPDVQARWRRSLEAYLSERS
                     SLVGLVVTMDIRRGVTQLDDTLLRWLEPREVPVAVLWTKADKLSRSAAIQQERELRKQ
                     LGSAVRATRFSALTGDGVATAQAWLEDWLEPPQK"
     gene            complement(7560..>9715)
                     /gene="polA"
                     /locus_tag="VM692_04230"
     CDS             complement(7560..>9715)
                     /gene="polA"
                     /locus_tag="VM692_04230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HUQ51404.1"
                     /translation="PGIDGVGPKTAVKWLQQFGDLETLKARAAEVSGKIGDRLRAALG
                     TLDLSKQLATIRCDVELPLKPADLALRPQDSPRLAALFERLEFTRLLRRVREEPVAQA
                     ATPPPPAAPVAERPPARYETIVTQDELERWCERIAAAQRVALDTETTNQAYMRAELVG
                     ISLAVTPGEAAYIPLGHRYPGAPDQLDRDAVLERLKPWLESEAPKVGHHLKYDAHIFA
                     NHGIALRGIEHDTMLESYVLNSTATRHDLDSVATFYLGPRTVKYEDVVGKGAKQITFD
                     QVDLDAATRYSAEDADLALQLHQTLWPKLAAEPALTRVYKEIECPLVRVLEDMEYAGV
                     MVDARLLREQSQELAEKMAATEKTAYSAAGGPFNLGSPKQLQEVLYDRLHLPVLGKTQ
                     KGQPSTSEDVLEQLAEEHELPKLILEYRSLTKLKSTYTDRLPVDIDPRTKRIHTSYHQ
                     AVAATGRLSSSDPNLQNIPIRTAEGRRIRQAFIAAPGFKLLAADYSQIELRIMAHLSG
                     DEGLLAAFASDQDVHRATAAEVFGAPLEAVTVDQRRSAKTINFGLIYGMSAFGLAKQL
                     GIERGQAQAYVDRYFERYPGVRRYMDETRASARRTGYVSTVFGRRLYLPDINARNPQV
                     RQYAERSAINAPMQGTAADIIKRAMVGVARWLASHEPQARLIMQVHDELVVEVPEHRG
                     AEIGAGVAQIMEAAADLRVPLRVDRGLGANWDEAH"
BASE COUNT         1684 a         3220 c         3204 g         1607 t
ORIGIN      
        1 cgacgtcgcg gcgccatttc tgacgccgat gaaaaccgca gtcttcgtcg cagtgttcat
       61 cgcgatgccg gtcgtgctgt ttcagatttg gcagttcgtc gcgcccggct tgtacagacg
      121 cgagaagcgt ctcgcgatcc cgctgatgat ttcgagcatc gtgctgttct actcgggcgt
      181 ggcgtttgcg tatttcatcg tgttcaaggt gatgttcggt ttcttcgcgg gcgttacgcc
      241 gagcggcgtg aacatgactc ccgacatcag cgcctacttg agcttcgtgc tcggcatttt
      301 tctgtcgttc ggcctggcgt tcgaagtgcc gattgcgacc gtgctattgc tttggagcag
      361 tctcgtctcg atgaaagcgc tcaagagcgc tcggccatac gtgtttctcg gcgcgttcgt
      421 cgcaggaatg ctgctggctc ccgacgtttt ctcgatgacc ctgatggcaa tcccgatgta
      481 tgcgctctat gagggcgggt tgctgatggc gcgtttcctg ctgccgatcg agaagaccgc
      541 ggtacaaacc tgacgatgaa gccaatcgac agcaagttgc cgacggtcgg cacgacgata
      601 tttacattga tgtcgcagct cgcccagcag caccgcgccg tcaatctttc gcaaggcttt
      661 cccgattttc gcccgcccgc ggcgctgctc gagctcgtcg aacgctgcct ccgcggccag
      721 taccaccaat acgcgttgat gccgggctcg atggcacttc gcacggcgat cgccgcgaag
      781 ctcgcggcga tgtacgacgt cgtcgcggac cccgtcaccg agatcacggt cacgagcggc
      841 gccaccgaag ccatcttcgg cgcgattcaa gcgctcgtgc gccgcgacga cgacgtcatc
      901 gtgttcgacc cgtgctacga ctcctacgag ccgtcgatcg cgctcgcggg cggacgggct
      961 gttcacctgc cgctgacgca acccgacttc gcgatcgact gggatcgcct tcgcagcgcg
     1021 ctgagctcac gcacgcgtct cgtcatcgtc aactcgccgc acaatccgag tggcgcgctg
     1081 ttgacgcgcg cggacctgga ccggctcgcg gaagcgctcc gcccctacga ttgttggttg
     1141 ctcagcgacg aagtctacga gcacgtcgtc ttcgacggct gtcgacacgc gacggtgctg
     1201 gcgcagcccg agcttgcgca gcgctgcttc gccgtgtttt cgttcggcaa gacctaccac
     1261 gcgaccggat ggaagctcgg gtactgcgtg gcgccgcccg cactgacggc cgagctgcgg
     1321 cgcgtccatc aatacgtgac cttcgcgtcg acgtcgccgc tgcagcaggc gctggccgag
     1381 tacatgacgc agcaccccga gcatcacgtc gggctgccgg cgttctatca ggagcgccgc
     1441 gactatttcg cacagctgct ggccgcgacg aaatttcgtc cgcgccgcgc cgccggaacc
     1501 tatttccagc tcgccgacta cagcgcgatc tcgacgctgc ccgacgtcga gttcgcccgc
     1561 tggctcacga cggagcatgg cgtcgcgacg attccgatct cggtgttcta ccgcgatcct
     1621 cccgacacga agctcgtgcg tttttgtttc gccaaggtga acgcgacgtt ggacgccgcc
     1681 gccgagcgcc tgcggcggct atgacgaacg cgtcagcggg ggctcggcgt cgtgtcgacg
     1741 ctctttgacg tcgcggttcg atacagccag taggcgagca atgcgagcgc cgcgagacct
     1801 agcccttcgc ctgccgtcga ctgaccgagg cggtagtcga gcggcagcgc gagcacgtcg
     1861 gcgcgttccg ttagccaaat cgggactgcg atcgcgatac ccatgagcgc ttcgcccgta
     1921 atcacgccgg ccgcgaacag catgcctttg cgaccgaggc gttcggcctc ctcgggcgag
     1981 ctgatcccga cgctcgactt gagcgtgcgc tcgcagaacc acgccagcag gccgccgacg
     2041 aagatcggca cggtgaggtc gagcggcaag tagatgccga cggccgcggc aagcaccggc
     2101 acgcggaacg ttgcacgctt ggccttcgct agctcgtcaa tgatgatgat gaaggcgccg
     2161 atcgccgcgc cgacgccgat ggtcggccaa ggcaacgagc cgccgaacag accctgtgcc
     2221 accgatgcca tcaaggtcgc ctgcggcgcc agtagcgctt gcggatgcgc gtccgtcggg
     2281 gctccgatgc catacgcgtg cgcgagaagg ttcaggactg gcgccatgac gagcgcgctg
     2341 gcgcacgcgc cgatcgcgag cattacctgc tgccgccacg gcgtggcgcc gacaatgtgg
     2401 ccgcacttca agtcttgaag gttgtcgccc gcgatgcagg ccgcgcagca gacgacggcg
     2461 ccgatcatga tcgccgcgac cgggccgatc gtcgatcgcg gacccatcag caacagcagc
     2521 acgccggccg aaaacaagat cgtgccgatc gtgatgccgg acaccggatt gttggacgtg
     2581 ccgacgaggc cggccatgta gccggagacc gagcagaaca ggaagcccgc gacgatcatg
     2641 atgatcgtca tcggtacgct gacggcgaac gagccgacga tgctctggta gagcgcgagc
     2701 aacggcagcg tgaatagcac gatgccgatc aacacgccct tcatcggcag gtcgcgttcc
     2761 gtgtgcgcga ccctcgtttg cgcgccggcg cgcgcggcgg tcagtccgct cttgatgccg
     2821 gacaccaacg acgtgcgcag actgaacagc gcccagaggc cgccgaccaa cattgcaccg
     2881 acgccgaggt agcgaatctg ccggctccac agaactcccg cggcggcttc ggcgtcgagg
     2941 cccgcgacgg cgttcgcgag atcgggattg ccgtcgagcc cgaacgcggc atagagcggg
     3001 atcgccacgt tccatgagat tacgccgccc agcagcacga cggccccgat gttcaggccg
     3061 acgatatagc cgacgccgag cagggccggc gaaagattcg tgccgaagta cgcgatgccc
     3121 ttgccgacgt agccggcgat agccgccgtg tcgggaatca aacccaggcc gctcgccgcg
     3181 gccagcttca acgctccgcc gccgagcgca gcgccaccca ggatcttgac gccttggctc
     3241 gggttttcgc cggctttcag cacctcggcg gccgctttgc cttccgggaa cgtgagcccc
     3301 tgatccacga tcaacgagcg gcgcaacggc acggagaaca ggacgccgag cagcccgccg
     3361 agagcggcga tcgcgacgac ccaaaggaag tcgaaatcgt cccagaagcc gaggatcacg
     3421 agcgccggaa tcgtaaagat cacgccggat gccagcgacg cgccggccga cccgcccgtc
     3481 tgcacgatgt tgttctcgag tattccgccg ccgccgagca cgcgcagcac ggccatggag
     3541 acgacggcgg ccgggatcgc ggaggcgatc gtcatacccg cgaacagacc gaggtaggtg
     3601 ttggcggccg ccaggaccat cgcgagcacg accgcaagga cgatcgcgcg cgcggtgagc
     3661 tgcggcgggg ccgacgtctg cgcgctcatc ggctcaacga atgccgccca tccagccgcg
     3721 gatgtagcgc gcgaaaatca tcatcaatag acccgcagcc gcgccgacga gcgtcatcgt
     3781 catgagcttg ctcggcagct cgctgacgtc ggccgcgctg atttcgccgc cgatcatgcc
     3841 ggcgagcagg ttgccgaccg cgaggccgag aaaccaagtg cccatcatct gacccgcgaa
     3901 gcgcggcggc gcgagcttcg tgatgttcga aaggccgacg ggggccaaac aaagctcacc
     3961 gaaagtgtgc aggaggtagg ccatcagcag ccacgtcggc gcgacgtcgt tgccggtcgc
     4021 gagcacgacg tcggcggcaa gcaccatgac gagaaagccg aggccgagca gcagcaaacc
     4081 gaagccgaac ttcagcggtg cggacggatc gaggttgcgt ttgccgagcg cgacccagag
     4141 ccacgcgaag aacggcgcaa acagcacgat gcagaccggg ttgacggact gataccacga
     4201 cgccggatgc acgccctcgg ggaacagccc cccgagcagc gatcgatcgg tgtagtcgac
     4261 ggcgaacaag ttgaacgtgg tcgcttgctg ctcgaagccg gcccagaaga cggcggagca
     4321 gaggaaaaac accgcgatca cgccgacacg cagacgctcc tcgcggctca gcccggcaaa
     4381 gaacagcacg tagccgaaga acgcaaacgc gatcgcgacc atcgccgtgc ccgccacctg
     4441 cgcgagtgtc gtcacgtcga cggggatcac gccgaagaac atcagccccg cggccgcgac
     4501 gagcaacgtc gatccgaccg caatccacaa ccacccgcgg cgttgctctg ccgcgctgac
     4561 gccgggcggt aagcggcccg catcgccgag atagtgctcg gtcaacttgt attgcacgac
     4621 gccgaggcac atgaacaggc ccgcgcagaa gaacccgccg cgccaattcc atccctcgcc
     4681 gatcgttcct gcgatcagcg gcgcgatcaa cgcaccgagg ttgatgccca tgtaaaagat
     4741 cgagaatccc gcgtcgcggc gcgcaccggg ctggccttcg tatagcgagc cgacgatcgc
     4801 ggagatgttt ggcttgagca agccgacgcc gatgacgatg acggcgagcc cgacgtagaa
     4861 gacggcgggc gacgccggca aagcgagaat gaagttgccg gccgcgatga tgatgccgcc
     4921 ccagaaaatg gcgttgcgct ggccgatcag tcgatcggcc atccacccgc cgggcaaccc
     4981 gaacaggtac acggcgcccg tgtatagccc gtagacggcg ccggcgacct cgcggtccat
     5041 gccgaagccc gggttcgccg cgctcgtggc tgcgaccagg aacaatacga gcaacgcgcg
     5101 cataccgtaa taggtgaagc gctcgaagaa ctcggtcatg aacaaagtcg cgagaccgcg
     5161 cggatgaccg aggaactcgc gggaagcggc ggacgcgtga gcggagcccg tggtcgccgt
     5221 ggagctcacc gtcgtttgcc gaatcgtcta cgcgccgggt gggccgccat tcaccgagtg
     5281 tgacccaact gcgcgcgcag cggcaaggcc aagggtcggc cgggagggcc tggcgcgcct
     5341 accgtcctgc gcgttcggag gcgacgagct cgccgatcaa ggcgagcagc tcttcgtagc
     5401 taccggccgc cggaccgtcg gtcgtgtatt tgccgttgac gatgatcgtt ggaacgctgc
     5461 cgatgcggta gcggcggttc agctcgtcgg cacgctgcag tttctcttgc acggcgaacg
     5521 agtcgaacgc cgtcttgaaa gcggcggcgt cgacgttgaa ccggccgaag aactcctgca
     5581 gcttttcttc cgagtcgagc cggttgccgc gctcgtgaat ctcgcggaaa aactctgcat
     5641 gcatctgcgc gcccttgccg agcgcctcgg ccgtgtagaa cgcccgcgcg tgcagctgca
     5701 acaaggggtt ccagacggcg ggaattcgca ggaaatttac gtagtcgggc ttctgcgcgc
     5761 gccattgctc gagcagcgga tcgaaggcga agcagtgtgg gcagccgtac cagaagacct
     5821 cggcgacctc gacctggtcg gggttcgagc tcgtcggctg cgtcggggac aggcgcatat
     5881 agtgcgtgcc catctcgaac cgccccgtcg tcggcgcgcc ggacgggtcg ctggccacga
     5941 gctccggctc gagcggatct tggctctgcg ccgttggcgc gaccgccgga tcggtctcgc
     6001 cgaccggctc agccgggccg ccgcacgcgc caagggcgaa cgtcgcgagc agccacagct
     6061 tcttgacgtc catcagtcga ggccctgcac gtagctcgcg agggcgcgga tctcgtcctc
     6121 gaacagctgg cccgcgacgt tacgcatcat ctgattgacg tcgacgtccg agcggcgatc
     6181 gccggacgcg tacagattca attgctttgt gacataggca gcgtgctggc cgccgacccg
     6241 aggatacgcc gcgagcgggt tgccgttgcc ctctggccca tggcatgcga tgcaggcggc
     6301 cacgccgcgc gccggaacgc cgccgcgata gatctgctgg ccgaggccca cgagcgtcgg
     6361 gtcggcgccc ttcggcgtcg gcgtctggga ggcgaaatag gccgcgacgt cgagcatgtc
     6421 ctgctccgag agcatgtccg cgaacggctt catcgtcacg tccgtgcgct cgccgttctt
     6481 gaacgctttg agctgacgca cgatgtagtt cgcgtgctga cccgcgagca tcggccaatc
     6541 gggggtcaag ctattgccgt cggcaccatg gcaggccgcg caagtcacgg acttggtacg
     6601 cccggcctcg acggacccct ccgctgccgc ggccgcgccc tcttgcgccg cgaccgacat
     6661 tgcggccaac gagccgccga gcaaagccgt gagcaagagg ccgaaacgcg tgcgtgtaag
     6721 ggtcgtcatt gacttcgtcg tcacttacaa aatcggccgc gattgtacac taaggccttc
     6781 gccccaagca gcgaccgcgc aggtcgtcag gtctcttgcc gaccgccata ttcctgaaga
     6841 gcgccgcgat cccaaacgat tttcccgcgg acgaggggcg cgaagttgcg ttcgtcggcc
     6901 gctcgaattc cggcaagtcc acggccgtga atatcctaac gggcacgaaa aagcttgctc
     6961 gtgtcagtaa gacgccgggc cgcacgcagc ttttgaactt cttttcattc ggcgaaggcc
     7021 ggcgcctcgt ggacctgccg ggctacggtt tcgcccgcgt cgcccccgat gtccaagcgc
     7081 gttggcgacg ctcgctggaa gcgtacctca gcgagcgttc atcgctggta ggcctcgtcg
     7141 tcacgatgga tattcgccgc ggcgttacgc agctcgacga cacgctgctg cgctggctcg
     7201 agccgcgcga ggtgcccgtg gccgtgctct ggacgaaagc cgataagctg agccgcagcg
     7261 cggcgatcca gcaggagcgc gagctgcgca agcaactcgg ctccgccgtc agggcgacgc
     7321 ggttttccgc tctgacgggc gatggcgtgg cgacggcgca ggcgtggctc gaggattggc
     7381 tcgagccgcc gcagaaataa agaagccccg ggtgcgggat tcaggggacg cacccggggc
     7441 atcgaacccg gcggggtggg ccaggttcaa agaccccgca ttagggagga tagcgggggc
     7501 acccttccta ggtgccgaac agttagtcca gccgtcttct gtaaaagttc caacggcaat
     7561 taatgcgctt cgtcccagtt ggctccgagg ccccggtcga cgcgcagcgg cacgcgtaga
     7621 tcggcagccg cttccatgat ttgcgcgacg ccggcgccga tttcagcgcc gcggtgctcg
     7681 ggcacctcga cgacgagctc gtcgtgcacc tgcatgatga gccgggcctg cggctcgtgg
     7741 cttgcgagcc agcgggcgac gccgaccatt gcgcgcttga tgatgtccgc ggctgtgccc
     7801 tgcatcggcg cgttgatcgc gctgcgctca gcatactggc gcacttgcgg gttacgcgca
     7861 ttgatatcgg gtagatacag ccgccggccg aagaccgtgc tgacatagcc cgtcctgcgc
     7921 gcgctggcgc gcgtctcgtc catgtagcgc cgcacgccgg gatagcgctc gaagtagcgg
     7981 tcgacgtaag cctgggcttg gccgcgctcg atgccgagct gcttcgcgag gccgaacgcc
     8041 gacattccgt agatcagccc gaaattgatg gtcttcgccg accgccgctg atcgaccgtg
     8101 acggcctcga ggggcgcacc gaacacctcg gcggccgtcg cgcggtggac gtcttggtcg
     8161 ctggcgaaag ccgcgagcaa gccctcgtcg ccggacaggt gcgccatgat tcgcagctcg
     8221 atctgcgagt agtcggcggc cagcagcttg aagcccggcg ctgcgatgaa cgcttgccgg
     8281 atgcgccgcc cctcggcggt gcggatcggg atattttgca aattcggatc cgacgacgac
     8341 agccggcccg tcgccgcgac ggcctggtga tacgacgtat ggatgcgttt cgtgcgcgga
     8401 tcgatgtcga cgggcagcct gtccgtgtag gtcgacttca gcttcgtcag actgcggtat
     8461 tcgaggatca gcttcggcag ctcgtgctcc tccgccaatt gctcgaggac gtcttccgac
     8521 gtagacggct gacccttctg ggttttgccg agcacgggca ggtgcaggcg atcgtagaga
     8581 acctcttgca gttgcttcgg cgatcccagg ttgaacgggc cgccggctgc gctatacgca
     8641 gtcttctccg tcgcggccat cttctcggcg agctcctggc tctgctcgcg cagcaagcgt
     8701 gcgtcgacca tgacgccggc gtactccatg tcttcgagca cgcggaccaa ggggcactcg
     8761 atctccttgt agacccgcgt cagcgccggt tccgccgcga gcttcggcca aagcgtttga
     8821 tgcagctgca gcgcgaggtc ggcgtcctcg gcggagtagc gcgtcgccgc gtcgaggtcg
     8881 acttggtcga acgtgatctg cttagcgccc ttgccgacga cgtcctcgta cttgaccgtc
     8941 cgcggcccga gatagaacgt cgccacggaa tcgagatcgt ggcgcgtcgc cgtcgagttc
     9001 agcacgtacg actcgagcat cgtgtcgtgc tcgatgccgc gcagcgcgat gccgtggttc
     9061 gcgaagatgt gcgcgtcgta cttcaggtgg tgcccgactt tcggcgcctc gctctcgagc
     9121 cacggcttca ggcgctcgag cacggcgtcg cgatccagct gatcgggcgc gccggggtag
     9181 cggtgcccga gcgggatgta ggcggcctcg cccggcgtga cggcaagcga gatgccgacg
     9241 agctcggcgc gcatgtaggc ctggttcgtc gtctcggtgt cgagcgccac gcgttgcgcg
     9301 gccgcgattc gctcgcacca acgttcgagc tcgtcctgcg tgacgatcgt ttcgtagcgc
     9361 gccggcgggc gctccgccac gggcgcggcg ggcggtggcg gcgtcgcggc ctgggcaacc
     9421 ggctcctcgc gcacgcgccg cagcaggcgc gtgaactcta gccgctcgaa cagcgcagcg
     9481 aggcgcggcg agtcttgcgg ccgcaaagcg agatccgccg gtttcagcgg cagctcgacg
     9541 tcgcagcgga tcgtcgcgag ctgcttcgac aggtcgagcg tgccgagcgc cgcgcgcagg
     9601 cgatcgccga tcttgccgct gacctctgcg gctcgtgcct tcaaggtctc gagatcgccg
     9661 aattgctgca gccacttcac ggccgttttg ggcccgacgc cgtcgatgcc cggaa
//
LOCUS       DATLEE010000196         4060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9157113, whole genome shotgun sequence.
ACCESSION   DATLEE010000196 DATLEE010000000
VERSION     DATLEE010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9157113"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VM692_04235"
     CDS             complement(<1..626)
                     /locus_tag="VM692_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HUQ51405.1"
                     /translation="MQNVDAALRARQERPVVKYLLIAASVLFLATFLIVPVVSVLGYA
                     FSQGLAVYMEALRAPDTLSAIRLTLTTAAIVVPLNTIFGVAAAWAIAKFDFRGKQILI
                     TLIDLPFSVSPVIAGLVFVLLFGAQGWFGPFFRDQGIQIVYALPGIVLATMLVTFPYV
                     ARELSPIMQMQGREAEEAAVSLGAGGWMTFFRVTLPSIRWGLLYGVIL"
     gene            complement(626..1471)
                     /gene="cysT"
                     /locus_tag="VM692_04240"
     CDS             complement(626..1471)
                     /gene="cysT"
                     /locus_tag="VM692_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HUQ51406.1"
                     /translation="MATARGWVSPSVLPGFGMTLGFAGLFLTFIVLIPLGALIIRASS
                     LPLDEFWRIATEPRALASYRLTFGASLLAATLNAVFGFIIAWTLVRYRFPGRRLVDAI
                     IDLPFALPTAVSGIALATVFAPNGWIGAQLAPLGIQVAYTWLGVVVALTLIGLPFVVR
                     SVQPALEEVDVSLEEAAESLGASRWMVFRRVILPTVLPSLATGFTLALARAVGEYGSV
                     IFIAGNLPLRTEITPLLIVIKLGQYDYLGAAALGLVMLGVSFVMLLSINAINGWRIRR
                     MAGRR"
     gene            complement(1504..2595)
                     /locus_tag="VM692_04245"
     CDS             complement(1504..2595)
                     /locus_tag="VM692_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HUQ51407.1"
                     /translation="MTTMRNLLLASAVLLGACGGSGSGDEGAANGELQLLNVSYDPTR
                     ELYREFNDRFVAKWLADTGNRVSVQMSHGGSGSQARAVIDGLNADVVTLALAWDIDNI
                     QAVSQRLPASWQSRLPNNSSPYTSTIVFLVRKGNPKGIRDWPDLVQEGVEIITPNPKT
                     SGGARWNYLAAWGFALERTLGSLDNLASADPAKLAAAEAEAEKFVGDIYRHVPVQDTG
                     ARAATNTFVQRKIGDVLLAWENEALLASREMGADAFDIVRPSVSVLAEPPVALIDSVV
                     DRKGTRAVAEAYLDYLYTPEGQDIVARNFFRPTDATVAARYTEQFPEMRLFTVDQVFG
                     GWARAQADHFADGGTYDRLFTAGSAAPRQ"
     gene            complement(2609..>4060)
                     /locus_tag="VM692_04250"
     CDS             complement(2609..>4060)
                     /locus_tag="VM692_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ51408.1"
                     /translation="ADAERRAREIAGLVRIDALLQRRPSQLSGGQQQRVALARALAHE
                     PRVLLLDEPFGALDAKIREELRRAIREIQRAVGITTMLVTHDQEEAFSMADRIGVMDR
                     GRLQEVGEPRALYQRPGTRFVAQFLGGANLLLGPYGFRDVRLGESYFEVDRWSEALRS
                     GDEAAVVVRPEDVVLAPMRDLLSVPVVGTGKIVDLKFVGASERLRLEISASETLASAQ
                     RPGAATFSIEAARGARDVEAMPLSLGQKVYVGFKHMHALPTPISSLRLIATGEHMPEE
                     LGRTPLVQQLAERMHIEPLHYNDMNTQPEEVRGLPIVKVAPDANVSSALELLERGARQ
                     VLVLTAPEPRADRMLIYVEPSSFGRDAVLAAVASLARHLPIDVGMLVREDEDGSYRDL
                     LDLRDASLKIHGLDIRTETFRGNATEAVRERLITSDQQTLLVIGLTAPERCSDLIDDL
                     QRLLKDTPPAAVLFVSGRDKSETQTSKSYAFSL"
BASE COUNT          732 a         1361 c         1362 g          605 t
ORIGIN      
        1 cagaggatca cgccgtacag caacccccag cgaatgctcg gcagcgtaac gcggaagaac
       61 gtcatccagc cgccggcgcc caagctgacg gcggcttcct cggcttcgcg cccctgcatt
      121 tgcatgatcg gactcagctc gcgtgctacg tacgggaacg tgacgagcat cgtggcgagc
      181 acgatgccgg gcagcgcgta gacgatttga atgccttgat cgcggaagaa cggtccaaac
      241 caaccctgcg cgccgaacag cagcacgaac acgaggcccg cgatgacggg cgacaccgag
      301 aacggcaggt cgatcagcgt gatgaggatc tgcttgccgc gaaaatcgaa cttcgcgatg
      361 gcccaggccg ccgcgacgcc gaagatcgtg ttcagcggca cgacgatcgc cgcggtcgtc
      421 agcgtcagcc ggatggccga cagcgtgtcg ggcgctcgca gcgcttccat atagacggcg
      481 aggccttgcg agaacgcgta gccgagcacg gacacgaccg gcacgatcaa gaacgtcgcg
      541 aggaacagca ccgacgcggc gatcagcaag tacttcacga cgggcctttc ctgccgcgcg
      601 cgcagtgcgg catcgacgtt ttgcatcagc gcctccccgc catacggcga atacgccagc
      661 cgttgatcgc gttgatcgac agcagcatca cgaacgacac cccgagcatg acgagcccga
      721 gtgcggcagc gccgaggtag tcgtactgcc ccagcttgat gacgatcagc agcggcgtga
      781 tctccgtgcg cagtggcaga ttgccggcga tgaagatcac ggagccgtat tcgccgacgg
      841 cgcgcgccaa ggccagcgtg aagcccgtcg cgagcgacgg cagaaccgtc ggcaggatga
      901 cgcggcggaa caccatccaa cggctcgcgc cgagagactc ggcggcttcc tcgaggctga
      961 cgtcgacctc ctcgagcgca ggctgcacgg agcgcacgac gaacggcaag ccgatcagcg
     1021 tcagcgcgac gacgacgccg agccacgtgt acgcgacttg aatgccgagc ggcgcaagct
     1081 gcgcgccgat ccagccgttc ggcgcgaaca ccgtcgcgag cgcaatgccg gacaccgcag
     1141 tcggcagcgc aaacggcaga tcgatgatgg cgtcgacgag ccggcggccc gggaagcgat
     1201 agcgcacgag cgtccaagcg atgatgaagc cgaacacggc gttcaaggtc gccgccagca
     1261 gcgaggcgcc gaaagtcagc cggtacgacg cgagcgcgcg cggctccgtc gcgatgcgcc
     1321 agaactcgtc gagcgggagg ctgctcgcgc gaatgatcag cgcaccgagc ggaatcaaca
     1381 cgatgaacgt caggaacaag ccggcgaagc caagcgtcat gccgaacccc ggcagcacgc
     1441 tggggctgac ccagcctcga gcggtggcca tgagctacag ccttgcgagc cgcgcgtgct
     1501 cgctcattga cgcggtgccg ccgaccctgc cgtgaacagc cggtcgtagg ttccgccgtc
     1561 ggcaaagtgg tcggcctgcg cacgtgccca gccgccgaaa acctgatcga cggtgaacag
     1621 ccgcatctcg ggaaactgct ccgtgtagcg cgctgcgacc gtcgcatccg tcggccggaa
     1681 gaaattgcgc gcgacgatgt cctggccttc gggcgtgtac aagtaatcga gatacgcctc
     1741 ggcgaccgcg cgcgtgcctt tgcgatcgac gaccgagtcg atcagcgcga cgggcggctc
     1801 cgcgagcacg ctgacggacg gccgcacgat gtcgaacgcg tcggcgccca tctcgcgcga
     1861 ggcaagcaac gcttcgttct cccaggcgag cagcacgtcg ccgatcttgc gctgcacgaa
     1921 cgtgttcgtg gccgcgcgtg cgcccgtgtc ttgcacggga acgtgccggt aaatgtcgcc
     1981 gacgaatttc tcggcctccg cctcggccgc ggcgagcttg gccggatcgg ccgacgccaa
     2041 gttgtcgagg ctgcccaagg tgcgctcgag cgcgaagccc cacgccgcca aataattcca
     2101 gcgcgcaccg ccggacgtct tcgggttcgg cgtaatgatc tcgacgcctt cctgaaccag
     2161 gtccggccaa tcgcggatgc ctttcggatt gcccttgcga acgaggaaaa cgatcgtcga
     2221 cgtgtacggc gagctgttgt tcggcagccg cgactgccac gacgccggca ggcgctgact
     2281 cacggcttgg atgttgtcga tgtcccaagc gagcgcgagc gtcacgacgt cggcgttgag
     2341 gccgtcgatg acggcgcgcg cttgactgcc cgagccgccg tgcgacatct gcaccgagac
     2401 gcggttgccg gtgtcggcca gccacttcgc gacgaaccga tcgttgaact cgcggtagag
     2461 ctcgcgcgtc gggtcgtacg agacattcag cagctgcagc tcgccgttcg cggcgccttc
     2521 atcgccggag cccgagccgc cgcaggcgcc gaggaggacg gcactcgcga ggagaagatt
     2581 gcgcatcgtc gtcatggtaa gcactcgctc aaagggagaa ggcgtacgat ttcgacgtct
     2641 gcgtctccga cttgtcgcgg ccgctgacga acaggaccgc ggccggcggc gtgtccttga
     2701 gcagccgttg caagtcgtcg atgagatcgc tgcagcgttc cggcgccgta aggccgatca
     2761 cgagcaaggt ctgctggtcg gacgttatga gccgctcgcg cactgcttcc gtggcgttgc
     2821 cgcggaacgt ctccgtgcga atgtcgaggc cgtgtatctt cagcgaggcg tcgcgcagat
     2881 cgagcaagtc gcgatagctg ccgtcctcgt cctcgcgcac gagcatgccc acgtcgatcg
     2941 gcaagtgccg cgcgaggctc gcgacggcgg ccagcacggc atcgcggccg aacgacgacg
     3001 gctcgacgta aatcaacatt cgatcggcgc gcggctccgg cgccgtcagc acgagcactt
     3061 ggcgagcgcc ccgctcgagc agctcgagcg cgctcgagac gttggcgtcg ggtgcgactt
     3121 tcacgatcgg cagcccgcgc acttcctcgg gttgggtgtt catgtcgttg tagtgcagcg
     3181 gctcgatgtg cattcgctcc gcgagctgct gcacgagcgg cgtgcgcccg agctcctcgg
     3241 gcatgtgctc gccggtcgcg atgagccgca agctgctgat cggcgtcggc agcgcgtgca
     3301 tgtgcttgaa gccgacgtaa accttctggc cgagcgacag cggcatcgcc tcgacgtcgc
     3361 gcgcgccgcg cgcggcttcg atcgagaacg tcgcggcacc cggccgttgc gcgcttgcca
     3421 acgtctcgct cgccgaaatc tcgagccgca accgctcgct ggcgccgacg aatttcaagt
     3481 cgacgatctt gccggtgccg acgacgggca cgctcaacag atcgcgcata ggcgcgagca
     3541 cgacgtcctc ggggcgcacg acgaccgcgg cttcgtcgcc gctgcgcagc gcctcggacc
     3601 agcgatcgac ctcgaaatac gattcgccga ggcgcacgtc gcggaagccg tagggaccaa
     3661 gcagcaagtt cgcgccgccc aagaactgcg cgacgaagcg cgtgccgggg cgttgataca
     3721 gcgcgcgcgg ctcgccgact tcttggagcc ggccgcgatc catgacgccg atgcggtccg
     3781 ccatgctgaa cgcttcttcc tgatcgtgcg tgacgagcat cgtcgtgatg ccgacggcac
     3841 gttggatctc gcgaatcgcc cgccgcagct cctcgcgaat cttcgcgtcg agggcgccga
     3901 agggctcgtc gagcagcaac acgcggggct cgtgtgccag cgcgcgagcg agcgcgacgc
     3961 gttgctgttg gccgcccgac agctggctcg gccgacgttg gagcagcgcg tcgatgcgga
     4021 caaggcctgc gatctcgcgc gcacgacgtt ccgcgtcggc
//
LOCUS       DATLEE010000197         2743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6072525, whole genome shotgun sequence.
ACCESSION   DATLEE010000197 DATLEE010000000
VERSION     DATLEE010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6072525"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VM692_04255"
     CDS             complement(<1..451)
                     /locus_tag="VM692_04255"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HUQ51409.1"
                     /translation="MHEYRPPTSDDKQIWDLWLTGLYQGTIVAADDAGIFEALAARPA
                     TIGELAQRLGFDTRATGIVLRLLAALRLLVMRQGSYRLADAANVYLVKSSPFYWGHMM
                     HVGVSEWHRNTTLAKLQQKGSSENAGPEGTPQPTGSGRRSDGWAAGKI"
     gene            547..1473
                     /locus_tag="VM692_04260"
     CDS             547..1473
                     /locus_tag="VM692_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ51410.1"
                     /translation="MKLVPLAVATACIALHCSPLAAQGIDYSKLEIKTTDLGSGVYLL
                     NWQGGDSLILVGDDGVLLVDTSVAAMGDKIEAAIAKVSGKPIKLIVNTHAHADHFGAN
                     EALAKGGATIVAHATLRERMANGFTAFNQPVPPAAPAALPTVIYDDAMTLHFDGETVQ
                     LIHPANAHTDSDTLVHFERANVIHASGTIGGDGGYVFFDSGSGGSLAGTIAAEDKMLS
                     LADDKTRIVADEGDPASTAMLRAQRDMLVTLRARVQKSIDEGKSEDEAVAAKPTKDFD
                     AQFVHQGNFLNGDAMTRLAYQSLKSAGPPTEH"
     gene            1481..2668
                     /locus_tag="VM692_04265"
     CDS             1481..2668
                     /locus_tag="VM692_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51411.1"
                     /translation="MRIAAVLAVLGAGVASGQTAPQLGDSPATAAAFGRIEHYLLNES
                     AASGRVAALLTLVAARELDLAHAWSEREQAARAANLEAAVIDVIRRNGALDALSRDDA
                     LLVDFGRQLYRNRYVRSETFAVLVDRLGKQGAFDAIMLLAYPAMAGVLERALERQPPE
                     DWDPARLPRMADAGTPTGRPGEFVALPNRPPLPSDVHEDSYYRFPLLKRQELDARTRE
                     LFDRVVGADKDTTPRGPVGMTFLSPQLVEPVQAINTALRTNGALGIRTAEIVVATTGR
                     EMNSQYQWTVHGAAADAAGAGQAVLDAIRDDKPVAQLDARDAVAITFTREIFREQRVR
                     DETFAQAVQLFGTQGTVELAALIGDYLMMTTVYNALGMRLRPEQAATLPHRAGAPIGA
                     EWR"
BASE COUNT          456 a          921 c          941 g          425 t
ORIGIN      
        1 cgatcttgcc tgcggcccag ccgtcggacc ggcgaccgga ccccgtcggc tgcggcgtac
       61 cttcgggacc cgcgttctcg gacgagcctt tctgctgcag cttcgcgagc gtcgtgttgc
      121 gatgccactc gctgacgcca acgtgcatca tgtggcccca atagaacggg ctcgacttca
      181 cgaggtagac gttcgcagcg tcggcgagcc gatagcttcc ttgtcgcatt acgaggagcc
      241 gcagcgccgc gagcaaccgc agcacgatgc cggtcgcccg cgtatcgaag ccgagccgct
      301 gcgcgagctc gccgatcgtt gcagggcgcg ccgcgagcgc ttcgaagatg cccgcatcgt
      361 cggccgcgac gatcgtgccc tggtagagcc ccgtgagcca cagatcccag atctgcttgt
      421 cgtcgctcgt cggcggtcga tactcgtgca cggctgtctc cacgcggatc agcccgcacg
      481 atagcagagc gcgtgcggcg ggactgaagt gctacgcttc gccatcgttc ggataccggg
      541 gactccatga agctcgttcc gctggccgtc gccactgcgt gcattgcgct tcactgttcg
      601 ccgcttgcgg cgcagggcat cgattactcg aagctcgaga tcaagacgac ggatctcggc
      661 agtggcgtct atctgctgaa ctggcagggc ggcgactcgc tgatcttggt cggcgatgac
      721 ggcgtgttgc tcgtcgacac gtccgtcgcg gcgatgggcg acaagatcga agccgcgatc
      781 gccaaagtca gcggcaagcc gatcaagctg atcgtcaaca cgcatgcgca cgccgaccac
      841 ttcggcgcca acgaggcgct cgcgaagggc ggcgcgacga tcgtggcgca cgcgacgctg
      901 cgtgaacgca tggcgaacgg cttcacggcg ttcaaccaac cggtcccgcc cgcggcgccg
      961 gccgcgttgc cgacggtcat ttacgacgac gcgatgacgc tgcacttcga cggcgagacg
     1021 gtccagctaa ttcatccggc gaacgcgcat acggacagcg acacgctcgt gcatttcgag
     1081 cgcgcgaacg tcatccacgc gagcggcacc atcggcggcg acggcggcta cgtatttttc
     1141 gacagcggct cgggcggctc gctggccggt acgatcgcgg cggaggacaa gatgctctcg
     1201 ctcgcggacg acaagaccag gatcgtcgcg gacgagggcg acccggcaag caccgcgatg
     1261 ctcagggcgc agcgcgacat gctcgtcacc ctgcgcgcgc gtgtgcagaa atcgatcgac
     1321 gagggcaaga gcgaggacga ggcggtcgcg gcgaagccga ccaaggactt cgacgcgcag
     1381 ttcgtgcatc agggcaactt cttgaacggc gacgcgatga cgcgcctcgc gtatcaatct
     1441 ctcaaaagcg ccggtccgcc gaccgagcac tgaggctcgc atgcgaatcg ctgctgttct
     1501 tgccgtgcta ggtgccggtg ttgcctcggg ccagaccgcg ccgcagctcg gcgactcgcc
     1561 ggcgacggct gcggcgttcg gccgcatcga gcactacctg ttgaacgaga gcgccgcgag
     1621 cggtcgcgtc gcagcgctgc tgacgttggt cgcggcgcgc gagctggacc tcgcgcacgc
     1681 gtggagcgag cgcgagcagg cggcgcgcgc ggcgaatctc gaggcagccg tcatcgacgt
     1741 gatccgccgc aatggcgctc tcgacgcgct gtcgcgcgac gatgcgctgc tcgtcgattt
     1801 cggccgtcag ctctaccgca accgctacgt gcgcagcgag acgttcgcgg tgctcgtcga
     1861 ccggctgggc aagcagggcg cgttcgacgc catcatgctg ctggcgtatc ccgcgatggc
     1921 cggcgtcctc gaacgcgcgc tcgagcgaca accgccggag gattgggacc ccgcgcggct
     1981 gccgcgcatg gcggacgccg gcacgcctac cggccggccc ggcgagttcg tcgcgctccc
     2041 aaaccggccg ccgctgccga gcgacgttca cgaggactcg tactatcgct tcccgctgct
     2101 gaagcggcag gagcttgacg cgcgtacgcg agagcttttc gaccgagtcg tcggcgccga
     2161 caaagacacg acgccgcgcg gacccgtcgg catgacattt ttgagcccgc agctcgtcga
     2221 gcccgtgcaa gcgatcaaca cggccctgcg cacgaacggc gcgctcggca tccgcaccgc
     2281 cgagatcgtc gtcgcaacga cgggccgcga gatgaacagc caatatcaat ggacggtcca
     2341 cggcgcggcg gccgacgctg cgggggcggg gcaggccgtg ctcgacgcga tccgcgacga
     2401 caagcccgtc gcgcaactcg acgcgcgcga tgcggtcgcg atcacgttca ctcgcgagat
     2461 ttttcgggag cagcgcgtgc gcgacgagac gttcgcgcaa gccgtgcagc tgttcggtac
     2521 gcaaggcacg gtcgagctgg ccgcgttgat cggcgactac ttgatgatga cgacggtcta
     2581 caacgcgctt ggcatgcggc tccgacccga gcaggctgcg acattgccgc atagggcggg
     2641 tgcgccgatc ggcgcggagt ggcgctgagg gtgaagccgc gcttcgtcgt cgccgcgcgc
     2701 catgaatcga cgtatcctgg cggcgggagt gagcggggat gtc
//
LOCUS       DATLEE010000198         3703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5439539, whole genome shotgun sequence.
ACCESSION   DATLEE010000198 DATLEE010000000
VERSION     DATLEE010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5439539"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1495)
                     /locus_tag="VM692_04270"
     CDS             complement(98..1495)
                     /locus_tag="VM692_04270"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HUQ51412.1"
                     /translation="MNKLAIVAAALLVVALLVAPWLVGSITEARVRERVAAIDASPTA
                     TAEVKSFDRGWFRSTAKIELSLVPDNVAQLADVTANLGPFAKLPIAIEFGHGPIAVLD
                     GVHFGWSKLVARPDTEAPGVTELQQTLGVPYLFEFRGRSSYTGRLRFDADSPPFTLPI
                     DEALLTFSGATLAGTFARQHLDADANISAVEFAWATGSFAIRGLFATADNELRQYVMP
                     GKGSLSIESIQASDTTQGTAPLFEAKNLKIQSDVALDAAQELAAMRLDYGLDSARVDD
                     TEVTAAALGVAVRNVDVAALEAYGAAATDAAAAGADPSTIKAALGPHLERALKAGPSL
                     TLDPLRFRYDDEPFEGRLEITTNTQRLPPAGTLDLDNPLLMLGLVNADAEVRVSKPLG
                     AELAKLAARLQLGADPSISPDQLEYMAEAQSGLMLTMLVGQGVLLEDGDGYRSSVQFR
                     DGAVTLNGNVLPFGL"
     gene            1621..2250
                     /locus_tag="VM692_04275"
     CDS             1621..2250
                     /locus_tag="VM692_04275"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HUQ51413.1"
                     /translation="MYIPKHFEQTDERALWEFVDAHAFGTLLTVTDGEPFASHLPFLV
                     DPDQRVLHCHVARANPQWQAIAASPRVLAIFAGPHGYVSPTWYAEPGVPTWNYAVVHA
                     RGEARTLDDAEHTRRHVEALAAKFERGRAAPWVPDYDVRRLKGIVGVEIRVDKLEGKF
                     KLSQNRSAADRERVVAELTSTGRDNDAALAQLMTAAVPRSPSSGTSKRR"
     gene            2331..3467
                     /locus_tag="VM692_04280"
     CDS             2331..3467
                     /locus_tag="VM692_04280"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUQ51414.1"
                     /translation="MRTTAIAFLSLAVLAAQLASAQPTAGEDSQHILTVDHYVPNQSI
                     VPAINGQTTQIYVRERVKPATVQRGAPSDDRVVLFVHGAGTPAEVAFDVPYGDYSWMA
                     YLADAGFDVFSMDMTGYGRSTRPNVMNEVCNLSAEQQTALGRTPCQATHNAQLTTIAS
                     DWDDLDAVVDYIRALRDVPRVSLVGWSLGGPRAGGYASRNANKVAKLVLLAPAYSRES
                     SQQPPQPSPAQGVAFNAQSHADFTANWDRQVGCPNQYEPAAAAAVWRAMLESDPVGAS
                     WGQGVRRAPNTTSWGFGKAAVERMQTPTLMIAGIHDKQVAPERVREYFADLGAESKVL
                     VDLGCSSHNAMWEANHLQLFRASLEWLTSGTVEGMKQGVVRLGY"
BASE COUNT          626 a         1230 c         1273 g          574 t
ORIGIN      
        1 agcgaggtca agctgcccga ccgcgaatcg cgcggcgccg ccgacgagcc ggcggcggcg
       61 gagcccgagc ctgcggccgc cgagccgaag aactgagcta cagaccgaac ggcagcacgt
      121 tgccgttcag cgtgaccgcg ccgtcgcgga attgcactga gctgcgatag ccgtcgccgt
      181 cctcgagcaa gacgccctgg ccgacgagca tcgtcaacat gaggcccgac tgcgcttccg
      241 ccatgtactc gagctggtcc ggagaaatcg aggggtcggc gccgagctgc agccgcgcgg
      301 cgagcttcgc gagctcggcc ccgagcggct tcgacacgcg cacctcggcg tcggcgttga
      361 cgagacccaa catcagcaaa ggattgtcga gatcgagcgt gcccgccggc ggcagacgct
      421 gcgtgttcgt cgtgatctcg aggcgccctt cgaacggctc gtcgtcgtag cggaaccgaa
      481 gcggatcgag cgtcaggctc gggccggctt tcagcgctcg ctcgaggtgc gggcccagcg
      541 cggctttgat cgtgctggga tcggctcccg ctgccgctgc gtcggtcgcc gcggcgccgt
      601 aagcctcgag tgccgcgacg tcgacgttgc gcacggccac gccaagcgcc gccgcggtga
      661 cctccgtgtc gtcgacgcgc gcggagtcga ggccgtagtc cagccgcatc gcggcaagct
      721 cctgcgcggc atcgagcgcg acatcacttt ggatcttcag attcttggcc tcgaacagcg
      781 gcgccgttcc ttgcgtcgtg tcgcttgcct gaatgctctc gatcgacagc gagcctttgc
      841 ccggcatgac gtattgacgc agctcgttgt ccgccgtcgc gaacagcccg cggatcgcga
      901 acgagcctgt cgcccacgcg aattccacgg cgctgatatt ggcgtcggcg tcgagatgct
      961 ggcgcgcgaa cgtgccggcg agcgtcgcac cggagaacgt cagcagcgcc tcgtcgatcg
     1021 gcagcgtgaa cggcggcgag tcggcgtcga agcggagacg cccggtgtag ctcgacctcc
     1081 cgcggaactc gaacagatag ggcacgccga gcgtttgctg gagctcggtg acgccgggcg
     1141 cctcggtgtc gggtcgcgcg acgagcttcg accaaccgaa gtgaacgccg tcgagcacgg
     1201 cgatgggccc gtgcccgaac tcgatggcaa tcggcagctt cgcgaacggg cccagattcg
     1261 cggtgacgtc ggcgagctgc gcgacgttgt cggggaccaa ggacagctcg attttcgccg
     1321 tgctgcggaa ccagccgcgg tcgaacgatt tgacctcggc tgttgccgtc gggctcgcgt
     1381 cgattgccgc gacgcgctcg cgcacgcgag cttccgtgat cgagcctacg agccatgggg
     1441 ccacgaggag cgcaacgacg agcaacgcgg ccgcaacgat ggcgagcttg ttcatacggt
     1501 tcctcgcagg cgctcagggc gtcgggatca tcccgaacgg ctcagcgcgg gtcaatgacg
     1561 caagtcgcgg ggcgcgccaa gctatgatcg cgcctctcat aggtagggaa cggccgtccc
     1621 atgtacatcc cgaagcactt cgagcagacg gacgagcgcg cgctgtggga gttcgtcgac
     1681 gcgcacgcgt tcggcacgct gttgacggtg accgacgggg aaccgttcgc gagccatcta
     1741 ccgttcctag tcgatcccga ccagcgcgtg ctgcactgcc acgtcgcgcg tgcgaatcca
     1801 caatggcagg cgatcgctgc gtcgccgcgc gtgctcgcga tcttcgcggg gccgcacggc
     1861 tatgtctcgc cgacctggta tgcggagccc ggcgtgccga cgtggaacta cgccgtcgtg
     1921 cacgcgcgcg gcgaggcgcg cacgctcgac gacgccgagc acacgcggcg ccatgtcgag
     1981 gcgctcgccg ccaagttcga gcgcggccga gccgcgccgt gggttcccga ctacgacgtg
     2041 cggcgcctca aaggcatcgt cggcgtcgag atccgcgtcg acaagctcga gggcaaattc
     2101 aagctcagtc aaaaccgctc ggccgccgat cgcgagcgcg tcgtcgccga gctgacttcg
     2161 acgggccgcg acaacgatgc ggctctggcg cagttgatga cggcggcagt gccgcgcagc
     2221 ccgtcttcgg ggacttccaa gcgtcgctag gcgctgtcgg gcaaacctgc gaggatggcc
     2281 accgttcggc gacttgcaag cgtcgccgcc aggttcacgg ggggatacag gtgcgcacca
     2341 cagcgattgc gttcttaagt ctggccgtgc tcgcagcgca gctcgcgagc gcgcagccga
     2401 ccgccggcga agacagtcag cacatcctga ctgtcgacca ctatgttcca aaccaatcga
     2461 tcgtgccggc gatcaacggg caaacgacgc agatctacgt gcgggaacgc gtgaagccgg
     2521 cgacggtcca gcgcggcgcg ccgagcgacg atcgcgtcgt gctgttcgtg cacggcgccg
     2581 gcacaccggc cgaggtcgca ttcgacgtgc cgtacggtga ctacagctgg atggcgtatc
     2641 tcgccgacgc gggcttcgac gtgttctcga tggacatgac aggctacggt cgctcgacgc
     2701 ggcccaacgt catgaacgag gtctgcaacc tatcggccga gcaacagacg gcgctcggcc
     2761 gcacgccgtg tcaggcgacg cacaacgcac agctcacgac gatcgcgtcc gattgggacg
     2821 atctcgacgc cgtcgtggac tacatccgcg cgctgcgcga cgtgccgcgc gtcagcctcg
     2881 tcggctggtc gctcggcggg ccgcgtgccg gcggctacgc ttcgcgcaac gcaaacaagg
     2941 tcgcgaagct cgtgctgctg gccccggcct attcgcgcga gtcgagccaa cagccgccgc
     3001 agccgagccc cgcgcaaggc gtcgcgttca acgcgcagtc gcacgccgac ttcacggcga
     3061 actgggaccg gcaagtcggc tgcccgaatc aatacgagcc tgcggcagcc gcggccgtct
     3121 ggcgcgccat gctcgaatcg gatcccgtcg gcgcgtcgtg gggtcaaggc gtgcggcgcg
     3181 cgccgaacac gacgagctgg ggtttcggca aagccgccgt cgagcgaatg caaacgccga
     3241 cgctgatgat cgccggcatt cacgacaagc aggtcgcgcc cgagcgagtg cgcgagtatt
     3301 tcgccgatct cggcgccgag agcaaagtgc tcgtcgatct cggttgctcc tcgcacaacg
     3361 cgatgtggga agcgaaccat ctgcagctgt ttcgcgcgtc gctcgagtgg ttgacgtcgg
     3421 gcaccgtcga aggcatgaag cagggcgtcg ttcggctggg ttactgatcg gccgcttaga
     3481 aacgggtatc ccggtcctgc ggcacgcagc gtagacttcg acgactcgca tcgagagaaa
     3541 gctaatgaaa aaagtgtttg cgacggcggc ggccgtgttg gcgttcggct tgctcggcac
     3601 gagcggcgcg caagaaggcc atccgttgaa aggctcatgg ctcggcgagt gggccggcaa
     3661 cacggttcac ggcgacaacg tgttgctgat cctcgactgg gac
//
LOCUS       DATLEE010000199         5057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4688301, whole genome shotgun sequence.
ACCESSION   DATLEE010000199 DATLEE010000000
VERSION     DATLEE010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4688301"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1477)
                     /gene="mnmE"
                     /locus_tag="VM692_04285"
     CDS             complement(128..1477)
                     /gene="mnmE"
                     /locus_tag="VM692_04285"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HUQ51415.1"
                     /translation="MALRRDDTIAALATAPGIGAIAVVRLSGPDARTIARVVTGADPQ
                     PRRAELCTFRDAAGAPLDRGLVLMFAGPHSFTGEDVVELHCHGGRVVSDALLAALFAL
                     GARAAEPGEFTLRAFLNDKIDLLQAEAIADLVASGSAQAARAAVRSLEGEFSAAVSRI
                     QQALTALRVRIEAWLDFPDEELPFDAAPQCAAELAAIIEQLAALNLRARSGRALRDGL
                     NVAIAGEPNAGKSSLLNRLAGYDAAIVTEIPGTTRDPLREHITLDGLPITVVDTAGLR
                     ETNDPVEREGVRRARVEVARADRLLWVADARVPLARAIAAARAASADAAALTVIANKI
                     DLTGAPPRIAVEEGTTVVYASALTGAGTDLLVAHLKQVAGVDVDAAGSFSARRRHLDA
                     LARTHGHLIATRSQLGAALELAAEHLRNAQSALSELTGELSSDDLLGEIFATFCIGK"
     gene            complement(1496..3151)
                     /gene="yidC"
                     /locus_tag="VM692_04290"
     CDS             complement(1496..3151)
                     /gene="yidC"
                     /locus_tag="VM692_04290"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HUQ51416.1"
                     /translation="MDNIRVFLWLTLLGMAWLTYTAWNADYAVTPDTAPPPPVAGPDN
                     GAPDTSLPTLAPSPDQPAQAPQIAAPAPTTGELIRVRTDVLDVRIASQGGDLVRADLL
                     QYRVSKNEPEPVVRLLDDMGPERWVFQTGLRSAVGGAEPNHLATFRTASSDYTLAPGQ
                     DELVVTLDWVGEGPISARKTYTFRRGQYEVDLTLTVTPTSGASWRGAPYAQMERVHVP
                     IKRSYLSIDSYSFKGPVLYDGDQFEKLDVQELAETPLTENGSGGWFASIQHHFLAAAV
                     PPSGETYRYDAAMRGPAYLLSAIGPVADVSTGSPLNATFKLFVGPKLQEQLQSVGNDL
                     ERTVDYGKTTLLAQPLFFLLATVERWVGNWGFAIILVTILIKAVFYKLTAMSGRSMAK
                     MRKIAPRMKALQERYKDDRQGLSQAMMELYKKEKVNPAAGCLPILIQMPFFFAFYWVL
                     VESVEMRQAPFMLWIHDLSARDPFYVLPVLMGTAMFFQTRLSPAPPDPVQARMMQIMP
                     IMFAALLIFLPAGLVAYQLTNTVLSILQQWRINKIVARESAAT"
     gene            complement(3144..3518)
                     /gene="rnpA"
                     /locus_tag="VM692_04295"
     CDS             complement(3144..3518)
                     /gene="rnpA"
                     /locus_tag="VM692_04295"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HUQ51417.1"
                     /translation="MLPDTAGFSPDQRLHSAAEFGRVFAEPARSSDRFFTVLARSSGR
                     TLARLGLTVSRRAARRAVDRNKLKRLARESFRHQRALPPWDFVVLARPGADKAERQAL
                     RASLDRHFEQLTKAAAAAERNG"
     gene            complement(3541..3675)
                     /gene="rpmH"
                     /locus_tag="VM692_04300"
     CDS             complement(3541..3675)
                     /gene="rpmH"
                     /locus_tag="VM692_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HUQ51418.1"
                     /translation="MKRTFQPSNLHRKRTHGFRARMATSAGRKVLASRRAKGRARLTP
                     "
     gene            3896..>5057
                     /gene="dnaA"
                     /locus_tag="VM692_04305"
     CDS             3896..>5057
                     /gene="dnaA"
                     /locus_tag="VM692_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HUQ51419.1"
                     /translation="MAENSAVYSSLWIQCARALEAELSEQQFNTWIRPLQLVEDGGLL
                     KLLAPNRFVVDWVKSHCLEQIRAWWTRHSEGAPEIQVEVGSRPTVRREAPARVEPEAR
                     VVATSTLGARLNKAFTFDSFVEGKSNQLARAAAFQVAQNPGNAYNPLFIYGGVGLGKT
                     HLMHAIGNLILTRDSTAKVAYVHSERFVSDMVRGLQHNTIAEFKRSYRSLDALLIDDI
                     QFFAGKDRSQEEFFHTFNALLEGQRQIILTCDRYPKEVAGLEERLKSRFGWGLTVAIA
                     PPELETSVAILMSKAAAEGETLPEEVAFFLAQRIRSNVRELEGALRRVLANSRFTGQP
                     ITLEFAKDALRDVLALQDKLVTIENIQKTVAEYFKIRVADLLSKRRSRSIARP"
BASE COUNT          897 a         1623 c         1671 g          866 t
ORIGIN      
        1 cattgtcgtg ggcacactgt tggttgtctt tcctcggaac cagcgggcct tcactggatg
       61 ctacaacgcg cgaattcagt gacttgtcgc gcaaccagga agccccagga ccgtgcggga
      121 aagtgtgcta tttgccgatg cagaacgtgg cgaagatctc gccgagcaaa tcgtcgctcg
      181 agagctcgcc ggtgagctcg ctcaaagcgc tttgcgcgtt gcgcagatgc tcggccgcga
      241 gctcgagcgc tgcaccgagc tgcgagcgcg tcgcgatcag atggccgtga gtgcgcgcca
      301 gcgcgtcgag atgtcgacgc cgcgcgctga acgatccggc agcgtcgacg tcgacgcccg
      361 ctacttgctt caagtgcgcg acgagcaggt cggtccccgc gcccgtcaac gccgacgcgt
      421 agacgacggt tgtgccctcc tccactgcaa ttcgtggcgg cgccccggtg agatcgattt
      481 tgttcgcgat cacggtcagc gcggcggcgt cggccgaagc cgcgcgcgcc gcagcgatag
      541 ctcgcgccaa cggcacgcgt gcatcggcca cccacagcag ccgatccgcg cgcgcgactt
      601 cgacacgcgc gcgccgcact ccttcgcgct cgacgggatc gttggtctcg cgtaggcccg
      661 ccgtatcgac gacggtgatc ggcaagccgt cgagcgtgat gtgctcgcgc agcggatcgc
      721 gcgtcgtgcc gggaatctcg gtgacgatgg cggcgtcata acccgcgaga cggttcagca
      781 ggctcgactt gcccgcgttc ggctcaccgg cgatcgcaac gttgagcccg tcgcgcagtg
      841 ctcgacccga acgcgcgcgc aggttgagcg cagcgagctg ctcgatgatg gcggcgagct
      901 cggccgcaca ttgcggcgca gcgtcgaacg gtagttcctc gtccgggaaa tcgagccagg
      961 cttcgatgcg cacgcgcagc gccgtcaacg cttgctgaat gcgcgacacg gcggcggaga
     1021 actcgccttc cagcgaacgc accgcggctc gcgccgcctg cgccgaaccg ctcgcgacga
     1081 gatcggcgat cgcttccgct tgcagcagat cgatcttgtc gttgaggaac gcgcgcagcg
     1141 tgaactcgcc cggctcggcc gcccgcgcgc cgagagcgaa aagcgccgcg agcaacgcgt
     1201 cgctgacgac tcgcccgccg tggcaatgca gctcgacgac gtcttcgccc gtgaacgaat
     1261 gcggccccgc gaacatgagc acgaggccgc ggtcgagcgg cgcaccggcg gcgtcgcgaa
     1321 acgtgcagag ctccgcgcgc cgcggctgcg ggtcggcgcc ggtcacgacg cgtgcgatgg
     1381 tgcgcgcgtc aggcccggaa agccgcacga cggcgatagc gccgattccg ggcgcagtag
     1441 cgagcgctgc gatcgtgtcg tcgcgccgca gtgccacctt cgttctccgc gcgtgttacg
     1501 tggctgccga ctcgcgggcc acgatcttgt tgatgcgcca ctgctgcaga atcgacagca
     1561 cggtgttcgt cagctgatac gcgacgaggc cggcgggcag gaagatgagc aacgccgcga
     1621 acatgatcgg catgatctgc atcatgcgcg cttgcacggg atcgggcggc gccgggctca
     1681 agcgcgtctg aaaaaacatc gcggttccca tcaacaccgg caacacgtag aacggatcgc
     1741 gcgccgacaa gtcgtggatc cagagcatga acggcgcctg ccgcatttcg acgctttcga
     1801 cgagcaccca gtagaacgca aagaaaaacg gcatctgaat caggatcggc aaacagccgg
     1861 cggccggatt caccttttcc ttcttgtaga gctccatcat cgcttggctc aaaccctggc
     1921 gatcgtcctt atagcgctcc tggagcgcct tcatgcgcgg cgcgatcttg cgcatcttgg
     1981 ccatcgaccg gccgctcatc gccgtaagct tgtagaacac ggccttgatg agaatcgtga
     2041 cgagaatgat cgcgaaaccc cagttgccga cccagcgctc caccgtcgcg agcaagaaga
     2101 acagcggctg tgcgagcagc gtcgtcttac cgtagtcgac ggtgcgctct aaatcgttgc
     2161 cgacgctctg caactgctct tgcagcttcg gcccgacgaa cagcttgaac gtcgcgttca
     2221 gcggcgagcc ggtgctgacg tcggcaaccg ggccgatggc gctgagcaaa taagcggggc
     2281 cgcgcatcgc cgcgtcgtat cgataggtct cgccgctcgg cggtacggcg gcggcgagga
     2341 aatggtgctg aatcgacgcg aaccagccgc ccgagccgtt ttccgtcaaa ggcgtttcag
     2401 cgagctcctg cacatcgagt ttctcgaact ggtcgccgtc gtagagcacc gggcctttga
     2461 acgagtacga gtcgatcgat agatacgacc gcttgatggg aacgtgcact cgctccatct
     2521 gcgcgtacgg cgcgccgcgc cagctggcgc cggaagtcgg agtgaccgtc aatgtcagat
     2581 cgacttcgta ttggccgcgg cggaacgtgt acgtcttgcg cgcgctgatc ggtccctcgc
     2641 ctacccagtc gagcgtgacg acaagctcgt cttgcccggg cgccagcgtg tagtcgctgc
     2701 tcgcggtccg aaacgtagcg agatgattcg gctcagcgcc gccgacggca ctgcgcaaac
     2761 ccgtttgaaa gacccagcgc tcgggtccca tgtcgtcgag caagcggacg acgggctcgg
     2821 gctcgttctt gctgacgcgg tattgcagca agtcggcgcg tacgaggtcg ccgccttgcg
     2881 atgcgatccg cacatcgagc acgtcggtgc gaacacggat gagctcgccg gtcgtcggcg
     2941 ccggcgcggc aatttgcggc gcctgcgccg gctgatcggg gctcggagcc agcgttggca
     3001 gtgacgtgtc cggtgcaccg ttgtcgggcc ccgccacggg tggcggcggc gcagtatcgg
     3061 gcgtcacggc gtagtcggca ttccatgccg tgtacgtgag ccacgccatg ccgagcagcg
     3121 tgagccacag gaatacgcga atgttatcca ttgcgctcgg ctgcggcggc cgccttcgtc
     3181 agttgctcga agtgtcggtc gaggctcgcg cgcagcgctt ggcgctccgc cttatccgcg
     3241 ccggggcgag ccagcacgac aaagtcccaa ggaggcaagg ctcgttggtg gcgaaaggac
     3301 tcgcgagcga ggcgcttcag cttgttgcga tccacggctc gtctcgctgc tcggcgtgaa
     3361 acggtcagac cgagccgcgc gagggtacgg ccgctcgaac gcgcgagaac agtgaagaag
     3421 cgatcgctcg atcgcgcggg ctcggcaaac acgcgaccga actccgcagc gctgtgcaat
     3481 cgttggtcgg gcgagaaccc tgccgtatcg gggagcaacg ccgcgccaaa attcgccccg
     3541 ttatggggtg agacgagcgc gacccttcgc gcgtcgactg gcgagaactt ttctgccggc
     3601 gctagtggcc atgcgtgcgc ggaagccatg tgtgcgtttg cggtgcaagt tgctgggctg
     3661 aaaggttcgc ttcatgtcga gtaccccgcg cgggcaaaaa gttcttgcga aaacaaggat
     3721 gtgggacggc tgggaagccg cgcactgtac gcggctggtc gtgcggctgt caaccgcacg
     3781 cgcgctgcgt gcgggggcag gcagcgcgcg ctttgcttgt ggataactgc gacggaggcg
     3841 tattagactg gcagcattcc ctgatcctca cgtccaaccc acttttcgag ctcccgtggc
     3901 agagaactcg gcggtttact cgtcgctctg gatacagtgc gcccgcgcgc tggaagcgga
     3961 gctctctgag cagcaattca atacctggat tcgtccgctg cagctcgttg aggacggcgg
     4021 tctgctcaag ctcctcgcgc cgaatcggtt cgtcgtcgat tgggtgaaga gccattgcct
     4081 cgagcaaatt cgggcttggt ggacacgcca cagcgaaggc gcgccagaga ttcaagtcga
     4141 agtcgggagc cgacccaccg tacgccgcga agcaccggcg cgagtcgaac ccgaggcgcg
     4201 cgtcgtcgcg acgtcgacgc tcggcgcccg gctgaacaaa gccttcacgt tcgatagctt
     4261 cgtcgaaggc aagagcaatc agctcgctcg cgcggccgca tttcaggtcg cgcaaaaccc
     4321 tggcaacgcg tacaacccgc tgttcatcta cggcggcgtc ggtctcggca agacccacct
     4381 gatgcacgcg atcggcaatt tgatcctgac gcgcgattcc acggcgaagg tggcgtacgt
     4441 gcattcggag cggttcgtca gcgacatggt gcgcggcctg cagcacaaca cgatcgcgga
     4501 gttcaagcgc agctatcgtt cgctcgacgc cttgttgatc gacgacatcc aatttttcgc
     4561 cggcaaggac cgctcgcagg aggagttctt ccatacgttc aacgcgttgc tggaaggcca
     4621 gcggcagatc atcctgacct gcgatcgcta tccgaaagag gtcgctggtc tcgaggagcg
     4681 gctgaaatcg cgctttggct ggggcctcac ggtcgccatc gcgccgccgg agctcgagac
     4741 cagcgtcgcg attctcatga gcaaggccgc cgcggagggc gagacgttgc cggaggaggt
     4801 tgccttcttc ctcgcacagc ggatccgctc gaacgtccgc gagctcgagg gcgcgctgcg
     4861 gcgagtgctg gccaactcgc gcttcacggg ccaaccgatc acgctcgagt tcgcgaaaga
     4921 tgcgctgcgc gacgtgcttg cgctgcagga caagctcgtc acgatcgaga acatccaaaa
     4981 gaccgtcgcg gagtatttca aaatccgtgt ggcggacctg ctgtcgaagc gccggagccg
     5041 ctcgatcgct cggccgc
//
LOCUS       DATLEE010000200        11013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_968703, whole genome shotgun sequence.
ACCESSION   DATLEE010000200 DATLEE010000000
VERSION     DATLEE010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11013
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968703"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1295)
                     /locus_tag="VM692_04310"
     CDS             complement(105..1295)
                     /locus_tag="VM692_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51420.1"
                     /translation="MTLKTLSATIAALLMISATATAYAQDGEGPPPARGARAGGGGGG
                     GPGGQAARPPTAEEIAQLPAERRTPAEQQTALAKNPNWKAPRTSWGDPNLQGSYSTDD
                     MRGIPRDRPQALGTQESLPQDQFLARAVQQFTGATRAATTETFLRNEWGTRTFGFTSL
                     VVDPPDGRTPAVTDAARARGQGNSSFSNDRFDSFEDFSLYDRCIGRGISNGMNAAIYG
                     NGVQVFQSPGYVTITYEMIHETRVIKLDNSPYPEIAQFTGNARGHWEGDTLVVESRGF
                     QDRTSVGGAPNSANMRTTERIRRVDPEMLEYRITINDPETYSAPFTVRTMWTTQPNYF
                     AGEYSCHEGNFAVGGGLAGERALDREREAAIAAGKPLPERSRANIYGNPQEGATIFDI
                     NAGE"
     gene            1571..2365
                     /locus_tag="VM692_04315"
     CDS             1571..2365
                     /locus_tag="VM692_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51421.1"
                     /translation="MRSPTLVLRLALGCAVPIVALTACSPPPASPAAASSAAAPTVPD
                     LSGVWMAFAVENPAGTGNAPQYSPGGQAKLDGFAAQFTAIPETGAWCYGTGMPSLMLS
                     TVSYPIEIVQHASRLVMLAELEMQVRRVYLDGRPHPSDFLSTGVGHSIGTWDGDTLVI
                     DTTLLSDWEIRPWPKSDQTHIVERVHLTKLGDIAARASGFVASVEKPINDDVLVVEMT
                     LTDPTYYAGPQQRTAYFQRMADTATLEYACSGEHWLEELEKNRASQ"
     gene            2547..3875
                     /locus_tag="VM692_04320"
     CDS             2547..3875
                     /locus_tag="VM692_04320"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ51422.1"
                     /translation="MFISKKHLPRRTFLKGLGATIALPLLDAMVPAFAATATGKPRLG
                     FVYVPHGMVMKRFTPDTAGKNFEFKDINRSLEPFRDRLSIVSRLHHRAADSTAVHSLS
                     PTTWLSGVRPKPTQGVDAFAGVTADQIAASAIGQDTLLPSMELATEDHSGLIGACDRD
                     YGCIYMNTLSWRTPTTPMPMEINPRKVFERMFGQGDSEAARLARRQEDRSILDAVTGQ
                     ATGLQRELGAHDRQSLDNYLEGVREIERRLTRAESQVATNADIAIPQAPSGVPFEYTD
                     HVRLMYDLLTLAYRADITRVFTFMMGREVSNRTYTQVGVTEGHHAVSHHQNKPEKLEM
                     LSRIQAFHIGLFGEFVAKLDEIPDGDGTLLDNTMLLSGSTMSNSNAHDHYPLPNIVVG
                     GGRGRLKGGQHIVAEDHAPMTNLLLTLLDKVDAGVESLGDSTGTIGEMSA"
     gene            3872..5401
                     /locus_tag="VM692_04325"
     CDS             3872..5401
                     /locus_tag="VM692_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ51423.1"
                     /translation="MIRSTLTRTLPAVALLTLLGVAAGAATPRLVQAVKTGDRTTALA
                     LARDAAEVAAAEADGTTALHYAVRQTDVELVDRLLKTGADVSAVNRYGVTPLKLAAVN
                     GDAKLLARLLDAGGDANAASADGETLLMTVARSGHVEAARLLLDRGAAVDARENWHGQ
                     TALMWAAAQGHPAMLRELLAHGADVNARSNEEKWERQVTSEPRDKWLPPGGLTALLFA
                     ARENCLACLPVLIEAGADIDATTPDGISGVVIALINGHFDVAGALVEAGTNPNLADYT
                     GRTGLYAAIDFNTMVKSNRPAPKVIENRLSGLDVARLLLEHGADPNAVLKRLPPYRAK
                     LDRGNDMMLGGGTTPFLRAAKAGDLPAMKLLLEHGADPKLAPTRSGINPLMAAAGLGT
                     AEQDTTGRLKTQQQAIDAIQLLLDLGLDINAQASDGQTAVHGAAMQGFDDVIRFLAAK
                     GAKLDTPDKDGFTPLDVALGKAGGFGFSGNEGVVRETTAALLTELMAANAAATGATAA
                     R"
     gene            5593..6777
                     /locus_tag="VM692_04330"
     CDS             5593..6777
                     /locus_tag="VM692_04330"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HUQ51424.1"
                     /translation="MTLDGVWNLSFWGYVAVTLALTHVTIASVTIFLHRHQAHRGLTL
                     HPVVSHFFRLWLWLTTGMVTREWVAVHRKHHARCETAEDPHSPQVYGIWRVLFRGVGL
                     YRVEALNKETLEIYGRGTPDDWLERHVYGQYRFTGLGITLLADLVLFGWVGLIIFAVQ
                     MAWIPFWAAGVINGVGHYLGYRNFETQDTSRNIVPIGILIGGEEFHNNHHAYGSSAKF
                     SNKWWEIDLGWLYIRALELVGLASVKKIAPRASFSRSTATIDLDTLRAVVTNRFYLLK
                     LYGRRVIAPVLHGLAEREPSFPRRQLARIRKLMIRENSGLREDPHVRQWLESALQGNP
                     TLRTVYSFMQRLKALSAQTTGKTESDLKRLQAWCAEAEASGIRVLGDFARQLQAYTPK
                     PV"
     gene            6890..7534
                     /locus_tag="VM692_04335"
     CDS             6890..7534
                     /locus_tag="VM692_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51425.1"
                     /translation="MSSEPPSGWQAPSGQRIGLSVVVALLVAAVVLVLAVLPAEYGID
                     PTGIGRALGLLELNAPATRTIEIRDVVGGNETVREAEIPDGGEPVPLPNPGVHQSEDG
                     PIQTRTLTLTLEPGQETEVKAVLRTSKVIVYHWQSEGGPAYCDLHGHDPAAGSEFFVR
                     YREDQEGATDVTGSLVAPFDGEHGWYWVNINAEPIKITLTVTGFFDDIVDYGIF"
     gene            7545..9152
                     /locus_tag="VM692_04340"
     CDS             7545..9152
                     /locus_tag="VM692_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HUQ51426.1"
                     /translation="MWQHNYDPLGNPLLSTLVAALPIFVLLLLIGILRKPAWMSALAG
                     LATGLLVAIAVYGMPANLAASSALMGAAFGLLPIGWIVYWAVVLYRITLETGNFEIIK
                     DSIGGVTGDRRLQAMLIAFAFGAFVEGAAGFGTPVAVAAAMLTGLGFSPFFAAGICLL
                     ANTAPVAFGSIGIPVTTLAATTGLPEAELSAWVGRICAPVSVFIPAYLVMVMGGFRGL
                     KGVLPAAIVCGVCFAGVQFTVSNYIGPQLVDILAALTAIVGLIVLLKVWHPKDNFVLA
                     GEHTAVAAPRQHGAGRTLLAWSPYLLLVLFVLVWGYPTTKAILARTSIPVPWPGLHNL
                     ITQIPPAVAAPTPYRAVYDFQWLVAAGTACALATVASALVLRVSVGMYFGILGRVLKQ
                     LAFSLLTIATVLGLAYVMNYSGAVVTLGIAFAATGVLFPFFSSILGWLGVFLTGSDTS
                     ANALFGNLQVVTANSLNLNPAMMAASNSAGGVMGKMISLTSLAVAVAATGMARSEEGK
                     LFRFTIKHSVFLAVVLGVITTIYAYTA"
     gene            complement(9182..>11013)
                     /locus_tag="VM692_04345"
     CDS             complement(9182..>11013)
                     /locus_tag="VM692_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUQ51427.1"
                     /translation="DAIGAELPDLVLTDVMMPRLDGFGLVAAIRADERTRSLPVIMLS
                     ARAGEEARVEGLSSGADDYLIKPFGARELLARVGSHLALATQRRQLEAELRYRNEQLE
                     TLLEAAPLGIYLVGPDFRIRAVNPVARRAFGDLPGGIDGRDFEEVMHLLREKPYADSM
                     VRIFRHTLATGEPYFTREHATSRPDRDVVEYFEWRLERITLPGGEAGLVCYFRDISPQ
                     VEARKAVEESREALKDADRRKDEFLATLAHELRTPLAPIHNALQILRVTGRDGAVARS
                     VHDMMERQVNHMVRLVDDLMEVSRITRGKIDLRKQRVDLAAIVGSAVETTRPLIDAAG
                     HLLTVDLGSEPLELDGDSVRLAQVFGNVLHNAAKFTPNGGQITVHAQRRGTEVVVSVR
                     DNGAGIRADMLPKIFEPFMQAERTYNRAQGGLGIGLTLARSIVALHGGTIEACSAGLG
                     AGSEFVVRLPLIQPVAVVLSTESNKRATPIAGQRILVVDDNVDSAESLSALLRCLGAD
                     VWTAHDGPAALECVRTQKLSAAVLDIGMPGMDGYEVARQTRAGPEGADLTLIALTGWG
                     NEEDRRRSREAGIDHHLVKPVELSVLESLLAARQPGQPANERG"
BASE COUNT         1789 a         3763 c         3593 g         1868 t
ORIGIN      
        1 cgcgagcccg tcggcgcacc gggatgtgcg ccgacgagcc cctgggacgg acttccggcg
       61 tgtctcgcga gtggccttcg atgaccctcg cgacctgacg agctctactc gccggcgttg
      121 atgtcgaaga tcgtcgcacc ctcttgcggg ttgccataga tgttcgcgcg cgaccgctca
      181 ggcagcggtt tgcccgccgc gatcgccgcc tcgcgctccc ggtcgagcgc ccgctcgccg
      241 gccaagccgc cgccgaccgc gaagttgccc tcgtggcacg agtactcgcc cgcgaagtaa
      301 ttcggctgcg tcgtccacat cgtgcgcacc gtgaacggcg cggagtacgt ctccggatcg
      361 ttgatcgtaa tgcggtactc gagcatctcg ggatcgacgc ggcgaatgcg ctccgtcgtg
      421 cgcatgttcg cgctgttcgg cgcgccgccg acgctcgtgc gatcctggaa gccgcggctc
      481 tcgacgacga gcgtgtcgcc ctcccagtgg ccgcgcgcat tgcccgtgaa ctgcgcgatc
      541 tcgggatacg gtgagttgtc gagcttgatg acgcgcgtct cgtggatcat ctcgtacgtg
      601 atcgtcacgt acccggggct ctggaatacc tgcaccccgt tgccgtagat cgcggcgttc
      661 atgccgttgc tgatgccgcg gccgatacag cggtcgtaga gcgagaagtc ctcgaacgaa
      721 tcgaatcggt cgttgctgaa cgagctattc ccttggccac gcgcacgagc cgcatcggtg
      781 acggccggcg tgcgaccgtc cggcggatcg acgaccagcg acgtgaagcc gaacgtccgc
      841 gtgccccatt cgttacgcaa gaacgtctcg gtcgtcgcgg cgcgcgtcgc acccgtgaac
      901 tgttgaacag cgcgggcaag gaactggtct tgcggcaacg actcttgagt gccgagcgcc
      961 tgcggccgat cccgcgggat gccgcgcatg tcgtcggtgc tgtagctgcc ttgcaggttc
     1021 gggtcacccc acgacgtccg cggggctttc caattcggat tcttggcgag cgcggtttgc
     1081 tgctcggcag gcgtgcgacg ctccgcgggc agttgcgcga tctcttccgc agtcggcggt
     1141 cgagccgctt ggccgccggg tccgccaccg cccccaccac ccgctcgcgc gcctctcgcc
     1201 ggcggcggtc cctctccgtc ctgcgcgtac gcagtcgcgg tggcggagat catgagcagg
     1261 gccgcgatcg tggccgacaa cgtcttaagg gtcatgtggt gctcctagaa ggcttgagcc
     1321 gcgagtcccc cgaccgcggt agtgcaaatt caagcacgcc gccgcgcccc agggaactcc
     1381 cccggaggcg aaaccagact tcttcaagta cgacgccggc gttagcggcc tcggtgtgtg
     1441 cgcttgccgc ccgacggtgg cagcttgatc gtcgcgggca aggagcggtt gatcgtgatg
     1501 ccggcgccgt aagcggagcc gcgactatac tgaccgcgtc gcgcgagacg cggcgaagag
     1561 agggaccgtc atgcgaagcc ccacgctggt tctgcgtcta gctcttggct gcgccgtgcc
     1621 gatcgtggcg cttacggcgt gctcgccgcc gccggcgtca cccgcggcag catcgtctgc
     1681 cgctgcaccg accgtcccag acttgtcggg agtctggatg gcgttcgcgg tcgagaaccc
     1741 ggccggcacc ggcaacgcgc cgcagtactc gcccgggggt caagcgaagc tcgacgggtt
     1801 cgccgcgcag ttcacggcaa ttccggagac cggtgcgtgg tgttacggca ccggcatgcc
     1861 gagcctgatg ctgtcgacgg tgtcgtaccc gatcgagatc gtgcagcacg cgtcgcggct
     1921 cgtgatgttg gcggagctcg agatgcaggt gcgccgcgtc tatctggacg gccgtccgca
     1981 tcccagcgat tttctatcga ccggggtcgg ccattcgatc ggcacctggg atggcgatac
     2041 gctcgtcatc gacacgacgt tgctgtcgga ctgggaaatc cggccgtggc cgaagtccga
     2101 ccagacccac atcgtcgagc gcgtccatct gacgaagctc ggcgacatcg ccgctcgggc
     2161 gagcggcttc gtcgcgagcg tggagaagcc tatcaacgac gacgtgctcg tcgtcgagat
     2221 gaccttgacg gaccctactt actacgccgg gccgcagcag cgcacggcct atttccagcg
     2281 aatggcggac acggccacgc tcgagtacgc ctgctccggc gagcactggc tcgaggagct
     2341 tgagaaaaat cgagcgtcgc agtagggctg caggccgcgc cggcagtcgc cttgcggcaa
     2401 tccgagctta ggaggggcaa cgggttacgt taaaatttgt ctgacctaac gagcactctt
     2461 cgagcgcgtc ccaattgggt tctcgcagag tattcgctac acttttcaat cgcttaatga
     2521 ccaccgcacg acggagaagg tccgggatgt tcatcagcaa gaagcacctg cctcgccgca
     2581 cgttcctgaa gggcttgggc gccacgatcg ccctgccctt gctcgacgcg atggtgccgg
     2641 cgttcgccgc caccgccacg ggcaagccgc ggctcgggtt cgtgtacgtg ccgcacggca
     2701 tggtgatgaa gcggttcacg ccggacacgg ccggtaagaa cttcgagttc aaggacatca
     2761 accgctcgct ggagccgttc cgcgaccgcc tttcgatcgt cagccggctg caccatcgcg
     2821 ccgccgattc gacggccgtg cactcgctga gcccgaccac ttggctgtcc ggcgtgcggc
     2881 cgaagccgac tcagggcgtc gacgcctttg ccggcgtcac ggcggaccag atcgcggcga
     2941 gcgcgatcgg tcaggacacg ttgctgccct cgatggagct cgcgaccgag gaccactccg
     3001 gtctgatcgg cgcctgcgac cgcgactacg gctgtatcta tatgaacacg ctatcttggc
     3061 gcacgccgac gacgccgatg ccgatggaga tcaacccgcg caaagtgttc gagcggatgt
     3121 tcggccaggg cgacagcgag gccgcgcgcc tcgcgcgccg tcaggaagat cgcagcatcc
     3181 tcgacgccgt gacgggccaa gcgacgggct tgcagcgcga gctcggcgct cacgaccggc
     3241 agtcgctcga caattacctc gagggcgtgc gcgagatcga gcgccgtctg acgcgcgcgg
     3301 agtcgcaagt cgcgaccaat gccgacatcg cgatcccgca agcgccgtcg ggcgtgccgt
     3361 tcgagtacac ggaccatgtg cgtctgatgt acgacttgtt gacgctcgcg tatcgcgccg
     3421 acatcacgcg tgtgttcacc ttcatgatgg gccgcgaggt cagtaaccga acgtacacgc
     3481 aagtcggcgt aacggaaggc caccacgccg tttcgcacca tcagaacaag ccggaaaagc
     3541 tcgagatgct gtcgcgcatc caggcgttcc acatcggcct gttcggcgag ttcgtcgcga
     3601 agctcgacga gattcccgac ggcgacggca cgttgctcga caacacgatg ctgctgtccg
     3661 gcagcaccat gagcaacagc aacgcccatg accactaccc gttgccgaac atcgtcgtcg
     3721 gcggcggccg cggccggctc aagggcggcc agcacatcgt ggccgaggat cacgcgccga
     3781 tgacgaacct gttgttgacg ctgctcgaca aggtcgacgc cggcgtcgag tcgctcggcg
     3841 acagcaccgg aacgatcggc gagatgtcgg cgtgattcgg tcgacgctaa cccgtacctt
     3901 acccgccgtc gcgctgctca cgctgctcgg cgtcgccgcc ggcgcggcca cgccgcggct
     3961 cgtgcaagcc gtcaaaaccg gcgatcgcac gacggcgctc gcgctcgccc gcgacgcggc
     4021 cgaagtcgcc gccgccgagg ccgacggcac gacggcactg cactatgccg tgcgccagac
     4081 cgacgtcgag ctcgtcgatc ggttgctgaa gaccggagcc gacgtgtcgg ccgtcaaccg
     4141 ctacggtgta acgccgctga agctcgccgc cgtcaatggc gacgccaagc tgcttgcgcg
     4201 actcctcgac gcgggcggcg acgcgaacgc ggcgagcgcc gacggcgaaa cgttgttgat
     4261 gacggtcgcg cgcagcggcc acgtcgaagc ggcccggctg ctgctcgatc gcggcgccgc
     4321 tgtcgatgcg cgcgagaact ggcacggcca gacggcgctg atgtgggccg ccgcgcaagg
     4381 ccatcccgcg atgctgcgtg agctgctcgc gcacggcgcc gacgtcaacg cccgctcgaa
     4441 cgaagagaaa tgggagcgac aggtcacgtc ggagccgcgc gacaagtggc tcccgcccgg
     4501 cggtctcact gcgttgctgt tcgcggcgcg cgagaactgc ttggcctgcc tgcccgtttt
     4561 gatcgaggcc ggcgcggaca tcgatgcgac gacgccggac ggcatcagcg gcgtcgtcat
     4621 cgccctgatc aacggccact tcgacgttgc cggcgcgctc gtcgaagccg gcacgaatcc
     4681 gaacctcgcc gactacacgg gccgcactgg gttgtacgcg gccatcgact tcaacacgat
     4741 ggtgaagtcg aaccgcccgg cgccgaaggt cattgagaac cgcttgagcg gtctcgacgt
     4801 cgcccggctg ttgctcgagc atggcgcgga cccgaacgcg gtgctgaagc ggctgccgcc
     4861 gtaccgcgcc aagctcgacc gcggcaacga catgatgctc ggcggcggca cgacgccgtt
     4921 cctgcgcgcg gccaaagccg gcgacttgcc tgccatgaag ctgctgctcg aacacggcgc
     4981 cgatccgaag cttgcaccga cgcgcagcgg catcaacccg ctgatggcgg cggcggggct
     5041 cggcacggcc gagcaggaca cgacagggcg attgaagaca cagcagcaag cgatcgacgc
     5101 aattcagctg ttgctcgacc tcgggctcga catcaacgcc caagcgagcg acgggcaaac
     5161 ggccgttcac ggcgccgcga tgcagggctt cgacgacgtc attcgcttcc tggccgccaa
     5221 gggcgcgaag ctcgacacgc cggacaagga cgggttcacg ccgctcgacg tggcgctcgg
     5281 caaggcaggc ggtttcgggt tctcgggcaa cgaaggcgtg gtccgcgaga ccacagccgc
     5341 tctgctgacc gagctcatgg ccgcgaatgc agccgccacc ggcgctacgg cggcccgctg
     5401 agctttgtca aagcgcggtt gcaaaaccgg gtaccggctc acatagttga agtcgccccg
     5461 gcgcacgacg cccgggacgg ctccggaacg aaggaaaacg agaagcgacg accggcgacg
     5521 atacgagtcg catgctacaa agctccggcc tctttgggtc ggaaccacgg gcttagagga
     5581 gtgacttgag gtatgacgct ggacggtgtg tggaatttat cgttctgggg ttacgtggcc
     5641 gtgacgctgg cccttacgca tgtcacgatc gcgagcgtca ccatctttct gcaccggcac
     5701 caagctcatc gcggcctcac gctgcacccc gtcgtcagcc acttcttccg cctctggctg
     5761 tggctgacca cgggcatggt gacgcgcgag tgggtcgccg ttcaccgcaa gcaccacgcg
     5821 cgctgcgaaa cggccgagga cccgcatagc ccccaggtct acggcatctg gcgcgtgctt
     5881 ttccgcggcg tcggcttgta ccgcgtggaa gcactgaata aggaaacgct cgaaatctac
     5941 gggcgcggca ctcccgacga ctggctcgag cgtcacgtgt acggccagta ccgatttacg
     6001 ggtctcggca tcacgttgct ggccgatctc gtgttgttcg gctgggtcgg cctcatcatc
     6061 ttcgccgtgc agatggcctg gattccgttc tgggccgccg gcgtcatcaa cggcgtcggc
     6121 cactacttgg gctaccgcaa cttcgagacg caggacactt cgcgcaacat cgtgccgatc
     6181 ggcatcctga tcggcggcga ggagttccat aacaaccacc atgcctacgg ctcgtcggca
     6241 aaattctcca ataagtggtg ggagatcgac ctcggctggc tttacatccg cgcgctcgag
     6301 ctagtcggcc tcgcgagcgt caagaaaatt gcgccgcgcg cgtcattctc acgctccacg
     6361 gccaccatcg acctcgacac gttacgcgcc gtcgtcacga atcgctttta cctgctgaag
     6421 ctttacggcc gccgcgtcat cgccccggtc ctgcacggcc tggccgaacg tgagccgagc
     6481 ttcccgcgcc gccagctcgc gcgcatccgc aagctcatga tccgcgagaa ctcggggctg
     6541 cgagaggatc cgcacgtgcg gcaatggctc gagagcgcct tgcaaggcaa tccaacgcta
     6601 cgcaccgtgt attcgttcat gcagcggctc aaagcgctgt ccgcgcaaac gacgggcaag
     6661 accgaatccg atctcaagcg gctgcaagcg tggtgtgccg aggccgaggc cagcggcatc
     6721 cgcgtgctcg gcgactttgc gcgccagtta caagcctaca cgccaaagcc cgtgtagcgc
     6781 gcagcgcgct ggcgtagccg ccggcactca acacggaaaa aactccggcg aataatttcg
     6841 gacgggtgtg tagactctcg aaaaaaagag cgccttgggg gggagttctt tgtcgtctga
     6901 gccaccatcg ggctggcaag cgccgtccgg tcaacgcata gggctgagcg tcgtcgtcgc
     6961 gctgctcgtc gcggccgtcg tgctcgtcct cgccgtgctg ccagccgagt acggcatcga
     7021 tcctaccggt atcggccgcg ccctcgggct cttggagctc aatgcgccgg cgacgcgcac
     7081 gatcgagatc agagacgtcg tcggcggcaa cgagaccgtt cgcgaggccg aaatccccga
     7141 cggcggcgag ccggtgccgc tgccgaaccc cggcgttcac cagtccgaag acggcccgat
     7201 ccaaacgcgc acgctcacgc tgaccctcga accgggccaa gagaccgagg tcaaggccgt
     7261 gctgcggacg agcaaggtca tcgtctacca ctggcagtcc gagggcggcc ccgcctactg
     7321 cgatctacac ggtcatgacc cggccgccgg cagcgagttc ttcgtgcgct atcgcgagga
     7381 ccaggaaggc gccacggacg tcaccggctc gctcgtcgcg ccgttcgacg gcgagcacgg
     7441 ctggtactgg gtcaacatca acgcagagcc catcaagatc acgctgacgg tcacgggctt
     7501 cttcgacgac atcgtcgatt acggaatttt ttagcgagac ttctatgtgg caacacaact
     7561 acgaccctct cggtaatccc ttgctctcga cgctcgtggc cgcactgccg atcttcgtgc
     7621 tgctgctgct gatcggcatc ttgcgcaagc ccgcgtggat gtccgcactc gcgggcctcg
     7681 ccaccggact cctcgtggcg atcgccgtgt acggcatgcc ggccaatctt gcagcgagct
     7741 cggcgctcat gggcgcggcc ttcggcttgc tgccgatcgg ctggatcgtc tactgggcgg
     7801 tcgtgctcta tcgcatcacg ctcgagacgg gaaacttcga gatcatcaaa gactcgatcg
     7861 gcggagtcac gggcgaccgc cgcctgcagg cgatgctgat cgctttcgcg ttcggcgcgt
     7921 tcgtcgaagg cgccgccggt ttcggcacgc cggtcgccgt tgcggccgcg atgctgacgg
     7981 gcctcggctt ctcgccgttc ttcgcggccg gcatttgctt gctcgcgaac acggcaccgg
     8041 tcgcgttcgg ctccatcggc attccagtga cgacgctcgc cgccaccacg ggcctccccg
     8101 aggccgagct ctcggcttgg gtcggacgca tttgcgcacc cgtctccgtg ttcatcccgg
     8161 cctatctcgt gatggtcatg ggcgggtttc gcgggctcaa gggcgtgttg ccggccgcaa
     8221 tcgtctgcgg cgtctgcttc gcgggcgtcc agttcaccgt gtcgaattac attggcccgc
     8281 agctcgtcga catcctggcc gcgctcacgg ccatcgtcgg cctcatcgtg ctgctgaagg
     8341 tttggcatcc gaaagacaac ttcgtgctgg ccggcgagca taccgccgtc gccgcgccgc
     8401 ggcagcacgg cgccggccgc acgttgctgg cctggtcgcc gtacctgctg ctcgtcctct
     8461 tcgtgctcgt ctggggctat ccgaccacga aggcgatcct cgcgcgcacc tcgattcccg
     8521 tgccgtggcc gggcctgcac aacctcatca cgcagattcc gccggcggtc gccgcaccga
     8581 caccgtaccg cgccgtctac gacttccagt ggctcgtcgc cgccggcacc gcctgtgcgc
     8641 tcgcgacggt ggcctcggcg ctcgtgctcc gcgtctcggt cggaatgtac ttcgggatcc
     8701 tcggccgcgt gctgaagcag ctcgcgttct cgctgctgac gatcgcaacc gtgctcggcc
     8761 tcgcctacgt catgaactac agcggcgccg tcgtgacgct cggcatcgcg tttgccgcga
     8821 ccggcgtgct gttcccgttc ttcagctcga ttctcggctg gctcggcgtg ttcctcacgg
     8881 gcagcgacac ctcggcgaac gcgttgttcg gcaacttgca ggtcgtgacg gcgaacagct
     8941 tgaatctgaa ccccgccatg atggccgcgt cgaactcggc cggcggcgtc atgggcaaga
     9001 tgatcagcct cacgagcctc gccgtcgccg tcgcggcgac cggcatggcg cggtccgagg
     9061 aaggcaagct attccgcttc acgatcaagc acagcgtgtt cttggccgtc gtgctcggcg
     9121 tcatcacgac gatctacgcc tacacggcct gagccggccg tggcggccgc gggcgcgcga
     9181 ctcagccgcg ctcgttcgcc ggctgaccgg gttggcgggc cgcgagcaag ctctcgagca
     9241 cgctgagctc gaccggcttc acgaggtgat ggtcgatgcc ggcctcgcgc gagcgccggc
     9301 gatcttcttc gttgccccag cccgtcagcg cgatcaacgt caaatcggcg ccctcggggc
     9361 cggcgcgcgt ttgccgcgcc acctcgtagc cgtccatgcc gggcatgccg atgtcgagca
     9421 cggcggccga aagcttctgc gtgcggacgc actcgagcgc cgcgggccca tcgtgagccg
     9481 tccagacgtc ggcgccgagg cagcgcaata gcgcgctcaa gctctcggcg gaatcgacgt
     9541 tgtcgtcgac gacgagaatt cgctgccccg cgatcggcgt cgcccgcttg ttcgattccg
     9601 tcgagagaac aaccgcgacg ggttgaatca gcggcaggcg cacgacgaac tcgctgcctg
     9661 cgcctagtcc cgcgctgcag gcctcgatcg taccgccgtg caacgcgacg atgctgcgcg
     9721 ccaacgtgag gccgatgccg agcccgccct gcgcgcgatt gtaagtgcgc tccgcctgca
     9781 tgaacggctc gaagattttc ggcagcatgt cggcgcgaat gccggcgccg ttgtcgcgca
     9841 cggagacgac gacctccgtg ccccgccgct gcgcgtgcac cgttatctgc ccgccgttag
     9901 gcgtgaattt agcagcgttg tgcagcacat tgccgaagac ttgcgcgagg cgcacggaat
     9961 cgccatcgag ctcgagcggc tcgctgccga gatcgacggt caacaagtgc cccgcggcgt
    10021 cgatcagcgg ccgcgtcgtc tcgacggcgc tgccgacgat cgcggcgagg tcgacgcgct
    10081 gcttacgcag gtcgatcttg ccgcgcgtga tgcgcgacac ctccatgagg tcgtcgacga
    10141 ggcgcaccat gtgattgacc tgccgctcca tcatgtcgtg cacgctgcgc gcgacggcgc
    10201 catcgcggcc cgtcacgcgc aagatttgca gtgcgttatg gatcggcgcc agtggcgtgc
    10261 gtagctcgtg cgccagcgtc gccaagaact cgtccttgcg ccgatcggcg tccttcagcg
    10321 cctcgcggct ctcctcgacg gccttgcgcg cctcgacctg cggcgagatg tcgcggaagt
    10381 aacagacgag cccggcttcg ccgcctggca gagtgatgcg ctcgagccgc cattcgaagt
    10441 attcgacgac gtcgcggtcc ggccgcgacg tggcgtgctc gcgcgtgaaa tacggttcgc
    10501 cggttgcgag cgtgtggcga aagatccgca ccatgctgtc ggcgtacggc ttctcgcgca
    10561 gcagatgcat gacctcttcg aagtcgcgac cgtcgatgcc gccgggcagg tcgccgaacg
    10621 cccgccgcgc aacgggattg accgcgcgga tgcggaagtc gggaccgacg aggtagatgc
    10681 cgagcggcgc cgcctcgagc agcgtctcga gctgctcgtt gcgataacgc agctccgcct
    10741 cgagctggcg gcgttgcgtc gccagtgcga gatgcgagcc gacgcgcgcc agcagctcac
    10801 gcgcgccgaa cggcttgatc aagtaatcgt cggcgccgga gctcaaacct tccacgcgcg
    10861 cctcttcgcc ggcgcgcgcc gacaacatga tgacgggcaa ggaacgcgtg cgctcgtcgg
    10921 cgcggatcgc ggcgacgagc ccgaacccgt cgaggcgcgg catcatgacg tccgtcagca
    10981 cgagatcggg cagctcggcg ccgatcgcgt cga
//
LOCUS       DATLEE010000201         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1714308, whole genome shotgun sequence.
ACCESSION   DATLEE010000201 DATLEE010000000
VERSION     DATLEE010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714308"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VM692_04350"
     CDS             <1..883
                     /locus_tag="VM692_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51428.1"
                     /translation="TNLAFWGTGNPAPDWDGSSRLGDNLYSNSVVALDVDSGELKWHY
                     QFTPHDELDYDSTQVPVLADITWRGTPRKVMLWANRNGIAYVLDRRTGKLLFGKPYVN
                     HNWWAGFDETGRLLRVPGMEIDKTPKLFRPHVHGAINWAPPSFSPRTGLLYVSHWENS
                     GIVAVEGQFPQSVGINKRQTAMGQVNLEPFFNNDDEAYGVVRAYHPDTLEPVWEFKMT
                     DITWGGVLTTAGDLAFGGGREGYFLALNARTGELLWRASLGGQINAGAMSYAVDGRQY
                     VSIAAGSALFSFALPKQ"
     gene            893..1777
                     /locus_tag="VM692_04355"
     CDS             893..1777
                     /locus_tag="VM692_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HUQ51429.1"
                     /translation="MQGDVLEVNVNELQPGRYQPRLQLREEGLAELAESIREQGVLQP
                     LIARVNSAAQGSADAKPYEIVAGERRWRAAQLAGLTTVPVILRELTDQSALAVALIEN
                     LQREDLNPIDQARSMARLIDEFDLTHEEIAKALGRSRAAVTNFLRLLDLADGVKDAMI
                     DGTIDMGHARALLPLEQEQQVELARKVAKFGWSVRKVESTVKALLTAPPSRAAPKALD
                     LQTRWLERQLARELGEMVSIRRGPNGDYVLQLGFADLAKLEASLQKLQELVRQIRAAA
                     GPRAREAAPDARARGGDS"
     gene            complement(1774..2154)
                     /locus_tag="VM692_04360"
     CDS             complement(1774..2154)
                     /locus_tag="VM692_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51430.1"
                     /translation="MSAQSFTPTAYVKDGCPFSFKFLTFAAETGLLGDLNIVRVRDGD
                     ANADTTKQMLSEKLGKPATFPTVEIEPGRYMTDSDKLIEHYAHRADVSPDELQLLSFY
                     KQTILPKLFEHYKLTSGAKSAAKN"
BASE COUNT          418 a          782 c          788 g          382 t
ORIGIN      
        1 gacgaatctc gcgttctggg gcaccggcaa cccggcgccc gactgggacg gcagcagccg
       61 gctcggcgac aacttgtact cgaactccgt cgtagcgctc gacgtcgaca gcggcgagct
      121 gaaatggcac taccaattca cgcctcacga cgagctcgac tacgactcga cacaagtgcc
      181 cgtgctcgcg gacatcacct ggcgcggcac gccgcgtaag gtcatgctct gggcgaaccg
      241 caacggcatc gcctacgtgc tcgaccgccg caccggcaag ctgctgttcg gcaagccgta
      301 cgtgaaccac aactggtggg ccggcttcga cgagacgggc cgtttgctgc gcgtgcccgg
      361 catggagatc gacaagacgc cgaaactgtt tcgcccacac gtgcacggtg cgatcaactg
      421 ggcgcctccg tcgttcagcc cgcgcacggg gctcttgtac gtctcgcatt gggagaactc
      481 agggatagtc gccgtcgaag ggcaattccc gcagtccgtc ggcatcaaca agcggcagac
      541 ggccatgggg caggtcaatc tcgagccgtt cttcaacaac gacgatgaag cgtacggcgt
      601 cgtgcgcgca tatcaccccg acacgctcga gcccgtctgg gaattcaaga tgacggacat
      661 cacttggggc ggcgtgctga cgacggcggg cgacctcgcg ttcggcggcg gccgcgaagg
      721 ctatttcctc gcgctgaacg cacgcacggg cgagctgctc tggcgcgcgt cgctcggcgg
      781 ccagatcaac gccggcgcga tgagctacgc cgtcgacggc agacaatacg tcagcatcgc
      841 ggcgggcagc gcgctgtttt cgttcgcgtt gccgaagcag tgagcgttgc cgatgcaggg
      901 tgacgtgctc gaggtcaacg tcaacgagct gcaaccgggg cgctaccaac cgcgcttgca
      961 gttgcgcgaa gaaggccttg ccgagctcgc cgagtcgatt cgcgagcagg gcgttctgca
     1021 gccgctgatc gcccgcgtca attcggctgc gcaggggagc gccgacgcga agccctacga
     1081 gatcgtcgcc ggcgagcgtc gctggcgtgc cgcgcagctg gccggcctta cgacggtgcc
     1141 cgtcatcctg cgcgagctga cggaccagtc cgcgctggcc gtcgcgctga tcgagaacct
     1201 ccagcgcgag gatttgaatc cgatcgacca ggcgcgctcg atggcacggc tcatcgacga
     1261 gttcgacctc acgcacgagg aaatcgccaa agcgctcggc cgctcacgcg cggccgtcac
     1321 gaattttctg cgcctgctcg acctcgccga cggcgtcaaa gacgcgatga tcgacggcac
     1381 gatcgacatg ggccacgcgc gcgcgttgct gccgctcgag caagagcagc aggtggagct
     1441 cgcgcgcaag gtcgcgaagt tcggctggtc ggtccgcaaa gtcgaaagca ccgtcaaggc
     1501 gctgctgacg gcgccgccga gccgcgccgc gccgaaagca ctcgacctgc agacgcgctg
     1561 gctcgagcgg cagctcgccc gcgagctcgg cgaaatggtc tcgattcgcc gcgggccgaa
     1621 cggcgactat gttttgcagc tcggcttcgc cgatctcgcg aagctcgaag caagcttgca
     1681 aaagctacag gagctcgtgc ggcagatccg cgcggcggcc ggaccccggg cccgcgaagc
     1741 cgctcccgac gcccgcgccc gcggcggcga ttcctagttc ttggccgccg atttggcgcc
     1801 gctcgtcagc ttgtagtgct cgaagagctt cggaaggatc gtctgcttgt agaacgacag
     1861 cagctgcagc tcgtcggggc tgacgtcggc gcggtgcgca tagtgctcga tcagcttgtc
     1921 cgaatcggtc atgtagcggc cgggctcgat ctcgacggtc gggaacgtcg ccggcttgcc
     1981 gagcttctcg ctgagcatct gtttggtcgt gtcggcgttg gcgtccccgt cccgcactcg
     2041 gacgatgttg aggtcgccga gcaaaccggt ctcggcggcg aaagtcaaaa acttgaacga
     2101 gaacgggcag ccgtctttga cgtaggcggt cggggtgaag gattgagcgc tcatctgcga
     2161 ttctccgggg ttgcagctgg tcggctgcag gctgcaacgt tcgtgccgac gctggggtag
     2221 cctcgcgcta cgctttcggt gccacgcgac gtggagacga agctactccg cgacgccgaa
     2281 cgccaacaag acgttgccgg gcatgccgcc gaagcgcgga taacccgagc ccacgaacac
     2341 catgccgtcg acgacgacgg cacccgctcc
//
LOCUS       DATLEE010000202         3967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3097365, whole genome shotgun sequence.
ACCESSION   DATLEE010000202 DATLEE010000000
VERSION     DATLEE010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097365"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VM692_04365"
     CDS             complement(<1..609)
                     /locus_tag="VM692_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HUQ51431.1"
                     /translation="MSFERSRREFLKQSAVLTALSAGAWCRPASAQERYVEAATAFGR
                     VRGVDAAGIKAFKGIPYGASTAGAARFRPPADPAAWTGVRDALAYGPSAPQREPGAAR
                     ADSGREVAAANLPPEGEDCLVLNVWTPAVRDSRRRPVMVWCHGGGFVTGSGSSPVTDG
                     TNLARRGDVVVVSINHRLGVLGFTNLAELGGGDFAQSGDAGML"
     gene            complement(656..2023)
                     /locus_tag="VM692_04370"
     CDS             complement(656..2023)
                     /locus_tag="VM692_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HUQ51432.1"
                     /translation="MTEPLAIQLAQFATATRTRDLPAVALERARMSLASTVASAAMGF
                     TIQSARIIRELDVRAGGAPSSTVWFDGTQLPPGAAARVNAVASDAAASDDSDLRSIAH
                     IGTIVSTVSMALAQQLGSTGADVLAAMVVGYEIAGRIDEALTPGRMQRGFHGSVSTVF
                     GGTVAAGRLLELTPQQMAHAIALAATSIGGMAISADTSCGREYHAGASAAAAVQAAFA
                     AQAGFTAELSVLEHPRGFLNAMGGQAIEDITRELGVSWDIVTDMAIKLMPGAHPFHAT
                     AEAAADAASLGKGDPDEIKEVFISSSVQWTKFKGAPHPRNLVDAAHSLYYFVAASIVD
                     GGFSWQHMDQGKMTDPVIAALQDKIVFDPDPPPLPDRFPHRHGGTVVIRMKDGEEFRS
                     TCKSPRGSGPRGIEWADVEAKYRALVPLAGVSSQRVGESLALIRAFDAPESVRRLVAL
                     LSLGR"
     gene            complement(2059..2442)
                     /locus_tag="VM692_04375"
     CDS             complement(2059..2442)
                     /locus_tag="VM692_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HUQ51433.1"
                     /translation="MPSVVLDASAVLALLANERGAEVVQRALGSAVMSAVNLAEVLTK
                     LGDRGMPQAEQRTVRMSLGLEIRPFNEASAWTASALRSATKKHGLSTGDRACLALALE
                     EGLPVLTADRAWSTLDVGVQIRTLR"
     gene            complement(2435..2893)
                     /locus_tag="VM692_04380"
     CDS             complement(2435..2893)
                     /locus_tag="VM692_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HUQ51434.1"
                     /translation="MDNLTKKQMDLIARPLRTKADKIRALARANVAPADIARFLNIRY
                     QHAYNVIQRAGLGDRIGESAAEPLAAAPISAKLDSNGCIELPESVRRAWGVTAGDEIL
                     LSLEGSELRLFTRKGGLRLAQGIVSRYLRPGESLADDLIRDRRHEAKDDA"
     gene            complement(3019..>3967)
                     /locus_tag="VM692_04385"
     CDS             complement(3019..>3967)
                     /locus_tag="VM692_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51435.1"
                     /translation="SAAWAQSGESFKAPRTSFGHPDLSGTWTNNNATPLQRPAQWAGK
                     ELLTDDELEAVQSSARQLEQDGDALFGDELILDAISGDPEPESHDTETGNYNGFWLPP
                     RDFERRTSLIIDPPDGRIPAYTPEAQAKRAAAAQRRREHPADGPWSRGLSERCITFGV
                     PRLTAAYSSVFEVVQSPNEVVFVMETIHNARVIPLDGRPHLSSAISQWDGNSRGHWEG
                     DTLVVETVGFSEKSDMMGASTGLKLVERFTRIAPDILQYDVTMSDPSTWTAPWTVRIP
                     MKQIEQPLIEYACHEGNLGMFGILAGARKQEADAARAAK"
BASE COUNT          617 a         1379 c         1338 g          633 t
ORIGIN      
        1 gagcatgccc gcgtcgccgg actgcgcgaa gtcgccgccg ccgagctcgg cgagattcgt
       61 gaagccgagg acgcccaagc ggtgattgat cgagacgacc acgacgtcgc cgcggcgcgc
      121 gaggttcgtg ccgtccgtga ccggcgacga gcccgagccc gtgacgaagc cgccgccgtg
      181 acaccagacc atgacgggcc gccgccggct gtcgcgcacg gccggcgtcc agacgttcag
      241 cacgaggcag tcttcgccct cgggcggcag gtttgccgcc gcgacctcgc ggccggaatc
      301 cgcgcgcgcc gcgccgggct cgcgctgcgg cgcgctgggg ccgtacgcga gcgcgtcgcg
      361 aacgccggtc cacgcggccg ggtcggccgg cgggcgaaag cgcgcggcgc ctgccgtgct
      421 cgcgccatag ggaatccctt tgaaggcctt gatgccggcg gcgtcgacgc cgcgcacgcg
      481 gccgaacgcg gtcgccgctt cgacgtagcg ttcctgcgcg ctcgcgggac ggcaccaggc
      541 tccggcggac aacgccgtca gcacggcgct ctgcttcaag aactcgcggc gtgaacgttc
      601 gaacgacatg gtctcccccc aatcgtcgct actgagcgcg agtgtactcc gacggctatc
      661 gccctaggct cagcagcgcg acaagccggc gcacggactc cggagcgtcg aacgcgcgga
      721 tcagcgccag gctctcgccg acgcgctgcg acgacacgcc ggccagcggc acgagcgcgc
      781 ggtacttggc ctcgacgtcg gcccactcga tgccgcgggg ccccgagccg cgcggcgact
      841 tgcaggtgct gcgaaactcc tcgccgtcct tcattcggat gaccacggtg ccgccgtgcc
      901 ggtgcgggaa gcggtccggc aacggcggcg gatcgggatc gaacacgatc ttgtcttgca
      961 gcgccgcgat gacgggatcc gtcatcttgc cctggtccat gtgctgccaa ctgaaaccgc
     1021 cgtcgacgat cgaagccgcg acgaagtagt agaggctgtg cgcggcgtcg acgagattgc
     1081 gcgggtgcgg ggctcccttg aacttcgtcc actgcaccga cgaggaaatg aagacctctt
     1141 tgatctcgtc tggatcgccc ttaccgagac tcgccgcgtc cgcggcggct tcggccgtcg
     1201 cgtgaaacgg atgcgcgccc ggcatcagct tgatggccat gtccgtcacg atgtcccacg
     1261 acacgccgag ctcgcgcgtg atgtcctcga tcgcctggcc tcccatcgca ttcaagaagc
     1321 cgcgcggatg ctcgagcacc gacagctcgg ccgtgaagcc cgcctgcgcg gcaaacgcgg
     1381 cttgcacggc cgcggccgcc gaggcgccgg cgtgatactc gcggccgcag ctcgtgtcgg
     1441 ccgagatggc catgccgccg atcgaggtcg cggccaacgc gatcgcatgc gccatctgct
     1501 gcggcgtcag ctcgagcagc cgccccgccg cgacggtgcc gccgaacacc gtcgacaccg
     1561 agccgtgaaa gccgcgctgc atgcgccccg gcgtcagcgc ttcgtcgatg cgccctgcga
     1621 tctcgtagcc gacgaccatc gcggccagaa cgtcggcgcc tgtggagccg agctgctgcg
     1681 cgagcgccat cgaaaccgtc gagacgatcg tgccgatgtg cgcgatgctg cgcaggtcgc
     1741 tgtcgtcact ggccgccgcg tcgctcgcga cggcgttgac gcgcgccgcc gcgcccggcg
     1801 gcagctgcgt gccgtcgaac cacacggtcg agctcggcgc accgccggca cgaacatcga
     1861 gctcgcgaat gatgcgcgcc gactgaatcg tgaagcccat ggcggcgctc gcgacggtgc
     1921 tcgcgaggct catgcgcgcg cgctcgagcg ccacggccgg caggtcgcgg gtgcgcgtgg
     1981 ccgtcgcaaa ctgagccaac tgaatcgcga gaggttctgt catgcagacg gcaatgtacc
     2041 ggagccagtc cgcgcgtgtc agcgcaaggt ccgaatctgc acgccgacgt cgagcgttga
     2101 ccaggcgcga tcggccgtaa gcaccgggag cccttcctcg agcgcaagtg caagacaggc
     2161 gcgatcgccc gtcgacagtc cgtgtttctt ggttgcgctt cgtaacgcgc tcgcggtcca
     2221 agccgaggct tcgttaaacg ggcggatctc caagcccagg ctcatgcgga ccgtgcgttg
     2281 ctccgcttgc ggcatcccgc ggtcgccgag cttggtgagc acctcggcca gattgacagc
     2341 ggacatcacg gcggagccga gcgcccgttg caccacctcc gcgcctcgtt cgttggccag
     2401 cagcgccaag acggccgaag catccagcac gacgctaggc atcgtccttg gcctcgtgcc
     2461 gccgatcgcg aatgaggtcg tccgccagac tttcaccagg ccgtagatag cgactcacga
     2521 tgccttgagc caggcgtaag ccgcccttgc gcgtaaagag ccgcagctcg ctgccctcga
     2581 gcgacagcag aatttcgtcg cccgccgtga cgccccatgc ccgacgcacg ctctccggta
     2641 gttcaatgca gccgttcgaa tcgagcttcg cgctgatcgg cgctgcggcg agcggttcgg
     2701 cggccgactc gccgattcgg tcgccaagcc cggcccgctg tatcacgttg taggcgtgct
     2761 gataacgaat gttcagaaag cgggcgatgt ccgccggcgc aacgtttgcg cgcgccaggg
     2821 ctcgtatctt gtccgctttg gttcgcagtg gacgcgcgat gaggtccatc tgcttcttgg
     2881 tcaggttatc catagttaac tagcccgtgt gggtagataa ctatactcgt gggtacctta
     2941 aatcaacaag aacggctggc acagcagcaa cggccgttgc gtcgaggtcg caacggctcg
     3001 tcggcgcaga aggaaaggct acttggcggc gcgcgcggcg tcggcttctt gtttgcgggc
     3061 gcccgcgagg atcccgaaca tgccgagatt gccctcgtgg cacgcgtact cgatcagcgg
     3121 ctgctcgatc tgcttcatcg ggatgcgcac cgtccaaggc gccgtccacg tcgaagggtc
     3181 ggacatcgtc acgtcgtact gcaagatgtc cggcgcgatg cgcgtgaagc gctcgacgag
     3241 cttgagcccg gtcgacgctc ccatcatgtc gctcttctcg gagaagccga cggtctcgac
     3301 gaccagggtg tcgccttccc aatgcccgcg cgagttgccg tcccactgag aaatggcgga
     3361 tgagagatgc ggccgcccgt cgagcgggat cacgcgcgcg ttgtgaatcg tctccatgac
     3421 gaacacgact tcgttcggcg actgcacgac ctcgaacacg ctgctgtacg ccgccgtcag
     3481 ccgcggcacg ccgaacgtga tgcagcgttc gctcaagccc cggctccacg gcccgtcggc
     3541 cgggtgctcg cgccgccgct gcgcggccgc ggcccgcttc gcttgcgctt cgggtgtata
     3601 cgccgggatg cgcccatccg gcggatcgat gatcagcgac gtccgtcgct cgaagtcgcg
     3661 cggcggcagc cagaagccgt tgtagttacc ggtctcggtg tcgtgcgact cgggctcggg
     3721 gtcgccggag atcgcgtcga ggatcagctc gtcgccgaac agcgcatcgc cgtcctgctc
     3781 gagctgacgg gcgctgctct gcacggcctc gagctcgtcg tcggtcagga gctccttgcc
     3841 ggcccattga gcgggacgct gcagcggcgt cgcgttgttg ttcgtccagg tgcccgacag
     3901 atcggggtgg ccgaacgacg tgcgcggggc cttgaacgat tcaccggact gggcccacgc
     3961 cgcggac
//
LOCUS       DATLEE010000203         4965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9162654, whole genome shotgun sequence.
ACCESSION   DATLEE010000203 DATLEE010000000
VERSION     DATLEE010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9162654"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VM692_04390"
     CDS             complement(<1..793)
                     /locus_tag="VM692_04390"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ51436.1"
                     /translation="MLRLRSTMLAAALVGAAALAHAQDNASWTFDTYTPGVCSANGGQ
                     HGGCPAGDLVRIHVVTVVDGLVRPWHLTFLPGSADYLVTELPGDLRIVRGGKLDAKPI
                     PGWPTDALRSRSMNSVVLHSDFARNRVLFISYVKQRDGGDTTIALARARFDGTKLERV
                     EDIFVADAWGTGATAGRAEVGPDGMIYLTVGDRDAANVTDDSSYRMLSQNLGSHVGKV
                     LRLRDDGTAPADNPFVGRAGAKPEIYTYGHRNAQGLAWHPETGELW"
     gene            991..2343
                     /locus_tag="VM692_04395"
     CDS             991..2343
                     /locus_tag="VM692_04395"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ51437.1"
                     /translation="MSDRHPRPAFISLVLMLLVSARGAMPGEAGAQPSAPESKADTLV
                     FDTWVPGPCSPNGQPQGQCPPGDLFRVRLVAIAEGLKSPRHIAFTPNGDLLVTELTEA
                     PAAGSSGNQAVQGRQGQVRIVRRGALAPLPLAGWPTASIEAGALWSVIVHPQFAANRS
                     VYLYYVKKRGEMSTRALARARLDGDALLDVREIFESDSWIPGGPMAGRAAFGPDGMIY
                     LTVNDHVEHDGRDGGNEPLLAQQLDNDVGKVLRVRDDGGIPADNPFVGRAGANGQVFT
                     YGQRNVTDFAWHPETGELWTTDIAPMGGDEINVLFAGKNYGWPLVSLGKNYNRKELSD
                     NHWYLPGMEMPVMYWTPSISPSTLTWYTGDKLAPWKGQLFVGALNGQTLLRVAFDQPM
                     PQTERRDSLFMSLGRRWRHVVQGPDGYLYALTEKRTLGGEPDPSDTTSGVVFRIEPAP
                     "
     gene            complement(2355..2840)
                     /locus_tag="VM692_04400"
     CDS             complement(2355..2840)
                     /locus_tag="VM692_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51438.1"
                     /translation="MTSSRATISTSIASCGAIRVDALITWTSNIQGWFTHSSWEYSSK
                     LQTIEVFTPRTDLQGELVGLEHEAVFYDEEALLQPIRNVRFLARQGALNDVPPNNLTH
                     CNQTLFVVNGRATPLIPGTETRYRVEDLYGRPWAAIWEEYFEQNMQRPQGEDVFDFER
                     R"
     gene            complement(2779..3111)
                     /locus_tag="VM692_04405"
     CDS             complement(2779..3111)
                     /locus_tag="VM692_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51439.1"
                     /translation="MASWRVRPQEKHMDLSTLRPSLRLLPRVTNAVALAVMLGCVSSV
                     VAQQSVGRGRPMAGGAEWVGAGVPFYNIDAATAKDGTVPQGVTPLARDIFTSDDFYVD
                     RELWRDPR"
     gene            3244..3909
                     /locus_tag="VM692_04410"
     CDS             3244..3909
                     /locus_tag="VM692_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUQ51440.1"
                     /translation="MRALVCVLLLSTCAALPAAAQSGRPSLPRDIDAQSYSRLPLVLK
                     ESLDEEGRRIFEAINGKESNTPRLGPPASTMHSLAAAEPYDRLNQLLRSANVIGPQFF
                     EISTLVPARELNQQYEWTAHERGARRVGVADEVIDVIKHGRPTSGLPEKEATAIEFGR
                     AMLRGNRQVPPELFAKMVSLFGERGTIEITMVMGDYAMTAMLLNAVNQQLPADWEPLF
                     PMP"
     gene            complement(3929..>4965)
                     /locus_tag="VM692_04415"
     CDS             complement(3929..>4965)
                     /locus_tag="VM692_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HUQ51441.1"
                     /translation="LTGIGEGGEPRTMDECASMLIGLDPFRIDQHWQRMLRGYFYPAG
                     REKIHSLGALDTALWDLKAKALDVPLWQLLGGKSRDHLELYATAFPRGPNGTLEDAAR
                     ACMDAGFRVYRHATDNPRERLVDRFKLVRQMHEDCIALHKITGDGGWAIDFHTELDPP
                     DAINLATLLEPLHPYFCEDLIRSEGVDSYVTMRERTRVPIAVGEQFGYKWDINTLIER
                     QLIDYARVTLPNAGGITELMKIIALAETHYVGMIPHFTGPISEAALVHCLTATSGVVL
                     MEMLGDGTRTWPYLPKVYDFKDGKLWPNDRPGLGVELDLDKLTLLGEYDTYRAGMLLN
                     RRPDGSFTNW"
BASE COUNT          839 a         1620 c         1676 g          830 t
ORIGIN      
        1 cccagagctc gccggtctcg ggatgccacg cgagcccttg cgcgttgcgg tggccgtacg
       61 tgtagatctc gggtttcgcg ccggcgcggc cgacgaacgg attgtcggct ggagccgtgc
      121 cgtcgtcgcg caagcgcagc actttaccga cgtggctgcc gagattttgc gagagcatgc
      181 gatagctcga gtcgtcggtg acattggccg cgtcgcgatc gccgaccgtc aagtagatca
      241 ttccgtcggg acccacctcc gcgcgtcctg ccgtcgcgcc cgtgccccag gcgtcggcga
      301 cgaatatgtc ctcgacgcgc tcgagcttcg ttccgtcgaa gcgcgcgcgt gcgagcgcga
      361 tcgtcgtgtc gccgccgtcg cgctgcttga cgtacgagat gaagagcacg cggttgcgcg
      421 cgaaatcgga gtgcagcacg acggagttca tgctgcgaga gcgcagtgcg tcggtgggcc
      481 agcctggaat cggtttcgca tcgagcttgc cgccgcgcac gatgcgcaaa tcgccaggca
      541 actccgtcac gagatagtcg gcgctgccgg ggagaaaagt cagatgccac ggccgcacga
      601 gcccgtcgac gacggtgacg acgtgaatgc gcacgaggtc gccggcgggg cagccgccgt
      661 gctggccgcc gttcgcggag cacacgcccg gtgtgtaggt atcgaacgtc cagctggcgt
      721 tgtcttgcgc gtgtgcaagt gccgcagcac cgaccagcgc tgctgcgagc atcgtcgatc
      781 gtaagcggag catgtccccc tccacacgtg atcggatgac gcagtgacca taggctcgtg
      841 cacgagacgg cgtcaactta ggagcgcgtt gactcgctcg cgccgcgcac gtagcgtggc
      901 tccgccacgc gcgcgcgcca tcgcgcgttg cgagcgcgca gtggcagacg gcgattacgg
      961 ccacaaggac gtgacaacgc gagagaagac atgagcgacc gtcacccgcg tcctgcgttc
     1021 atcagtctgg tgctgatgct tctagtctcg gctcgaggcg cgatgccggg ggaggcaggc
     1081 gcgcaaccga gcgcgccgga gtcgaaggcc gacacgctcg tcttcgatac ctgggtgccg
     1141 gggccctgct cgccgaacgg gcagccgcag ggacaatgtc cgccgggcga cttgttccgc
     1201 gtgcggctcg ttgcgatcgc cgaagggctc aagagcccgc gtcacatcgc gttcacgccc
     1261 aacggcgatc tcttggtcac cgagctcacg gaagcgcctg ccgccggatc ttccggcaat
     1321 caagcggtgc agggccgcca agggcaagtg cgcatcgtgc gccgcggcgc gctcgcgccg
     1381 ttgccactcg cgggttggcc gaccgcgagc atcgaagccg gcgcgctgtg gtctgtcatc
     1441 gtgcatccgc agttcgctgc gaatcgttcg gtgtatttgt actacgtcaa gaagcgcggc
     1501 gagatgtcga ctcgcgcgct cgctcgcgcg cggctcgacg gcgacgcgct gctcgacgtg
     1561 cgcgagatct tcgagtccga ctcgtggatt cccggcgggc cgatggcggg gcgcgcggcg
     1621 ttcggccccg acggcatgat ctacctgacc gtgaacgacc acgtcgagca cgatgggcgc
     1681 gacggcggca acgagccgct gcttgcgcag caactcgaca acgacgtcgg caaagtcctg
     1741 cgcgtgcgcg acgacggcgg cattcccgcc gacaatccgt tcgtcggtcg cgcgggcgcg
     1801 aacggccaag tgttcactta cggccagcgc aacgtgacgg acttcgcctg gcatcccgag
     1861 accggcgagc tctggaccac cgacatcgcg ccgatgggtg gcgacgagat caacgtcttg
     1921 ttcgccggca agaactacgg ctggccgctc gtctcgctcg gcaagaacta caaccgcaag
     1981 gagctcagcg acaaccactg gtacctgccg ggcatggaga tgcccgtgat gtactggact
     2041 ccgtcgatca gcccgtcgac gctcacgtgg tacaccggcg acaagctcgc accctggaag
     2101 gggcagctct tcgtcggcgc actgaacggc cagacgctgc tgcgagtggc gttcgatcag
     2161 ccgatgccgc aaacggagcg gcgcgactcg ttgttcatgt cgcttgggcg ccgttggcgg
     2221 cacgtcgtgc aagggccgga cggttatctc tatgccctga cggagaagcg tacgctcggc
     2281 ggcgaaccgg acccgagcga cacgacgagc ggcgtcgtgt ttcgcatcga gccggcccca
     2341 tgacgacaac ggaactaccg gcgttcgaag tcgaagacgt cctcgccctg cggccgctgc
     2401 atgttctgct cgaagtactc ttcccaaatc gcagcccaag gccggccgta gagatcctcg
     2461 acgcgatagc gggtctccgt gccgggaatc aacggcgtcg cccggccgtt cacgacgaac
     2521 agcgtttgat tgcagtgcgt caggttgttc ggcggcacgt cgttgagcgc gccctgccgc
     2581 gccaagaaac gcacgttgcg aatcggttgt agcagcgcct cctcgtcgta gaagacggct
     2641 tcgtgctcga ggccgacgag ctccccctgc aaatccgtgc gcggcgtgaa gacctcgatc
     2701 gtctgcagct tgctcgaata ctcccagctc gagtgcgtga accacccctg gatgttggag
     2761 gtccaggtga tgagcgcgtc aacgcggatc gcgccacagc tcgcgatcga cgtagaaatc
     2821 gtcgctcgtg aagatgtcac gtgcgagcgg cgtgactcct tggggcaccg tgccgtcctt
     2881 ggccgtcgct gcgtcgatgt tgtagaaggg cactccggcg ccgacccact cggcgccgcc
     2941 cgccatgggg cgaccgcggc cgacactttg ttgcgcgacg accgatgaaa cgcagccaag
     3001 catgacggcc agcgcgacgg cgttcgtcac tctcggcagc agtcgcaagc tcgggcgtag
     3061 cgtcgaaaga tccatgtgct tctcctgcgg ccggacgcgc caactagcca tgcagaacgc
     3121 ggagcggtca ggctcggagc tgccggtccg cgcaagatgg caaggtacga agcgtagttc
     3181 cgtggcgcct ccggaacggt tcggctagcc tacgagcggt tctgcgcaca ggaggcgtcg
     3241 ccgatgagag cgctagtctg cgtgttgttg ctgtccacgt gcgcggcgtt gcctgccgct
     3301 gcgcagtcgg gtcgcccgtc gttgccgcgc gacatcgatg cacagtcgta ctcgcgcttg
     3361 ccgctcgttc tgaaagaatc gctcgacgaa gaaggccgac gcatcttcga ggcgatcaac
     3421 ggcaaggaga gcaacacgcc tcggcttggt ccgcccgcca gcacgatgca cagccttgcg
     3481 gctgccgagc cgtacgatcg gctgaaccag ctgctgcgca gcgcgaacgt catcgggccg
     3541 cagttcttcg agatcagcac gctcgtgccg gcgcgcgagc tcaatcagca atacgagtgg
     3601 accgcgcacg agcgcggtgc gcggcgcgtc ggcgtcgccg acgaagtcat cgacgtcatc
     3661 aagcatggcc gcccgacgtc gggcttaccc gagaaggaag cgaccgcgat cgagttcggc
     3721 cgcgcgatgc tgcgcggcaa tcggcaagtg ccgccggagc tcttcgccaa gatggtgtcg
     3781 ctgttcggcg agcgcggcac catcgagatc acgatggtca tgggcgacta tgcgatgacc
     3841 gcgatgctgc tcaacgccgt gaaccagcag ctgccggcgg actgggagcc gttgttcccg
     3901 atgccgtagc gcagacccgg gggcgaccct accagttcgt gaacgagccg tcgggccgac
     3961 ggttcagcaa catgccggcg cgatacgtgt cgtactcgcc gagcagcgtg agcttgtcga
     4021 ggtcgagctc gacgccgagc cccggccgat cgttcggcca gagcttgccg tccttgaagt
     4081 cgtagacctt cggcaaatac ggccaggtgc gcgtgccgtc gccgagcatc tccatgagca
     4141 cgacgcccga ggtggccgtc aggcagtgca cgagcgcggc ctccgagatc ggccccgtga
     4201 agtgcgggat catgccgaca tagtgcgtct cggcgagcgc gatgattttc atgagctccg
     4261 tgatgccgcc ggcattcggc aacgtgacgc gcgcgtaatc gatgagctgc cgctcgatca
     4321 gcgtgttgat gtcccatttg tagccgaact gctcgccgac ggcgatcggg actcgcgtgc
     4381 gctcgcgcat cgtcacgtac gaatcgacgc cttcgctgcg gatcaggtcc tcgcagaagt
     4441 acggatgcag cggctcgagc agggtagcga ggttgatcgc gtcgggaggg tcgagctccg
     4501 tatggaagtc gatcgcccaa ccgccgtcgc cggtgatctt gtgcagcgcg atgcagtcct
     4561 cgtgcatttg ccgcacgagc ttgaagcggt cgacgagacg ctcgcgcgga ttgtcggtcg
     4621 catgacgata aacccggaag ccggcgtcca tgcacgcgcg cgccgcgtcc tcgagcgtgc
     4681 cgttcggccc gcgcggaaac gccgtcgcat agagctcgag atggtcgcgc gatttgccgc
     4741 cgagcagctg ccacagcggc acgtcgagcg ccttcgcttt caagtcccag agcgccgtgt
     4801 cgagcgcgcc gagcgagtgg atcttctcgc ggccggccgg gtagaagtag ccgcgcagca
     4861 tgcgctgcca atgctgatcg atacggaacg ggtcgaggcc gatcagcatg ctggcgcact
     4921 cgtccatcgt cctcggctcg ccgccttcgc cgatgccggt gagcc
//
LOCUS       DATLEE010000204         3193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1715967, whole genome shotgun sequence.
ACCESSION   DATLEE010000204 DATLEE010000000
VERSION     DATLEE010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715967"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VM692_04420"
     CDS             <1..752
                     /locus_tag="VM692_04420"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ51442.1"
                     /translation="DPRSPRETNGPKGLGDYTIPAANKFQADGDPKTLGEIYAYGLRN
                     AHRLSWDLTDGTMYVGDIGMSNIEEINIIVNGGNYGWMKREGYWENGMIRPGGALNQL
                     YELPADVLSGRNRDEFIYPVAMYDHDEGVAITGIVPYHGRIEALRGKLIFGDVNRGRL
                     FATDIAAAKAANDGVPQTVAPIEEIQLYVRGPNGQRIYTSFRELVEGTMGTSLARTDL
                     HVGRSSDGEIFLTSRQDGMIRMLVPDSGRDR"
     gene            770..1558
                     /locus_tag="VM692_04425"
     CDS             770..1558
                     /locus_tag="VM692_04425"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HUQ51443.1"
                     /translation="MAENPYAAPRTHVQDARTSFPDGDFIADGRGVAAGNGWRWIVDA
                     WTFTADQRLTFVGLFVLLLVVQLAANFLPVIGPLAVSLFTPVLVGGFVLGCEAVRRGE
                     PLEISYFFAGFSRHTGKLVALGALSVAFGILAGVIMILIVGSAFLPILLGTAEPAPEE
                     VLNLLLPMLLAVLVIMALSLPLSMAMLFATPLIVLENFEVGAALKSSFAACLKNVLPF
                     LVWSVAILILGLLAAIPLLLGWFLLAPIVMVSLYLSYRDIFHDI"
     gene            complement(1569..2774)
                     /locus_tag="VM692_04430"
     CDS             complement(1569..2774)
                     /locus_tag="VM692_04430"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HUQ51444.1"
                     /translation="MLSEVGNHLWQSTLTLAAVAVVAALLRRDGAHVRYWLWWAASVK
                     FLVPFSLLTLLGSAIGADLWLAGSAPVNPAEWRSTLDLLEPMRTPRVWTTATVALLSV
                     WSFGFGAVAAIWAARALEVRALLRTSRPYTAALPTTAAEVEVRTTTSFVEPVLVGISK
                     PVLLLPHGIAERLTTRQLSAVLAHELAHWRRRDNLTAAAHMLVESVFWFHPLVWWLGG
                     RLVEERERACDEAVLRAGHDGRTYAEGILSVCEHYVASKLPCAAGVTGADLQRRVVEL
                     TRSRLMSKLPIRKKVLLGTFALVTLLSPIVFGVAQGDRAYVPIVRINPEYPPSALAAR
                     LEGEVNLQFTIAANGAVKDIVVVESTAPEFELPAMAALQKWRYQPVTENDQPVEVPGV
                     RTIIRFALK"
     gene            complement(2780..>3193)
                     /locus_tag="VM692_04435"
     CDS             complement(2780..>3193)
                     /locus_tag="VM692_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HUQ51445.1"
                     /translation="RFYYTRSRTPAYMPLPKLSKLELKIMEALWTKGASSVREIQESF
                     PAKRRPAYTTVQTIVYRLEAKNALKRTKKIGNAHIFEAVISQTAARRKLIDDVLGLFG
                     GRAQPLMSHLIESGQLTLQDIEEARAALREATTKE"
BASE COUNT          524 a         1060 c         1034 g          575 t
ORIGIN      
        1 tcgacccgcg cagcccgcgc gaaacgaacg gcccgaaggg cctcggcgat tacacgatcc
       61 cggccgccaa caagtttcaa gccgacggcg acccgaagac gctcggcgag atctacgcgt
      121 acggcttgcg caacgcgcat cgtctttcgt gggacttgac ggacggcacg atgtacgtcg
      181 gcgacatcgg catgagcaac atcgaagaga tcaacatcat cgtcaacggc ggcaactacg
      241 gttggatgaa gcgcgaaggc tactgggaga acggcatgat ccgtcccggc ggcgcgctga
      301 accagctcta cgagctgccg gccgacgtgc tgagcgggcg caaccgcgac gagttcatct
      361 atcccgtcgc gatgtacgac cacgatgagg gcgtcgcgat cacgggcatc gtcccgtacc
      421 acggccgcat cgaagcgctg cgcggcaagc tgatcttcgg cgacgtgaat cgcggccggt
      481 tgttcgccac ggatatcgcc gcggcgaagg ccgccaacga cggcgtgccg cagacggttg
      541 caccgatcga ggaaatccag ctctacgtcc gcggcccgaa cggccaacgc atctacacgt
      601 cgttccgcga gctcgtcgag ggcacgatgg gcacgtcgct ggctcgcacg gacttgcacg
      661 taggtcgcag ttctgacggc gaaatcttcc tcacgtcgcg gcaggacggc atgatccgca
      721 tgctcgtgcc cgattcgggc agggatcgtt agcttcgagg tagatgccaa tggccgagaa
      781 cccgtacgcc gccccgcgca cccatgtgca ggacgcgcgg acctcgtttc ccgacggcga
      841 tttcatcgcc gacgggcgcg gcgtcgcggc cggcaacggc tggcgctgga tcgtagacgc
      901 ctggacgttc acggccgatc agcgtttgac gttcgtcggc ctcttcgtct tgctcttggt
      961 cgtccagttg gccgccaact tcttgcccgt gatcggcccc ctcgccgtga gcctgttcac
     1021 gcccgtgctg gtcggcggtt tcgtgctcgg ctgcgaggcc gtgcgtcgcg gcgagcccct
     1081 cgagatcagc tattttttcg cggggttcag tcggcacacc ggcaagctcg tggcgctcgg
     1141 cgcgctgtcg gtggcgttcg gcatcctggc tggcgtgatc atgatcctca tcgtcggctc
     1201 ggcgttttta ccgatcttgc tcggcaccgc cgagcctgct cccgaggaag tgctgaacct
     1261 gctgctgccg atgctgctgg ccgtgctcgt catcatggcc ctgagcttgc ctctgtcgat
     1321 ggcgatgttg ttcgcgacgc cgttgatcgt gctcgagaac ttcgaagtcg gcgcggcgct
     1381 gaagtccagc tttgcggcgt gcctcaagaa cgtcttgccg tttctcgtgt ggagcgtggc
     1441 gattctgatc ctcggcctgc tggccgcgat cccgctgctg ctcggctggt tcctgctcgc
     1501 gccaatcgtg atggtctcgc tctacctctc gtatcgcgac atcttccacg acatttagcg
     1561 gccggcggct acttcaaggc gaaccgaatg atcgtgcgca caccgggcac ctcgacgggc
     1621 tgatcgttct cagtgaccgg ctgatagcgc cacttctgca gcgctgccat ggccggcagc
     1681 tcgaactcgg gcgccgtcga ctcgacgacg acgatgtctt tgaccgcgcc gttcgccgcg
     1741 atcgtgaatt gcaaattgac ttcgccttcg agccttgcgg cgagagcgct cggcggatac
     1801 tcgggattga tgcgcacgat cgggacgtat gcgcgatcgc cttgcgcgac gccgaagacg
     1861 atcggcgaca gcagcgtgac gagcgcaaac gttcccaaca ggaccttttt acggatcggc
     1921 agcttactca tcaggcgact cctcgttagt tcgacgacgc gccgttgcag atcggcgccg
     1981 gttacgcctg cggcgcacgg cagcttcgaa gccacgtagt gctcgcagac actgagaata
     2041 ccttcggcgt acgtgcgccc gtcgtgaccc gcgcgcagga cggcctcgtc gcacgcgcgc
     2101 tcgcgctcct cgacgagccg cccgccgagc caccacacga gcgggtggaa ccaaaacacg
     2161 gactcgacga gcatgtgcgc ggccgccgtg aggttgtcgc gacgccgcca gtgggcgagc
     2221 tcgtgcgcga ggacggcgct gagttgcctc gtcgtcaggc gctccgcgat gccgtgcggc
     2281 aacagcagca ccggcttcga gatcccgacg aggacgggct cgacgaacga agtcgtcgtg
     2341 cggacttcga cctcggcggc cgtcgtcggt agcgccgcgg tgtacggacg tgatgtgcgc
     2401 agcagcgccc ggacctcgag cgcgcgcgca gcccagattg cagcgacggc gccgaagccg
     2461 aacgaccaca ccgagagcag ggcgaccgtc gccgtcgtcc acacgcgcgg cgtccgcatc
     2521 ggctccagca agtcgagcgt cgaacgccat tcggccggat tgacgggcgc ggagccggcg
     2581 agccacaagt cggcgccgat cgcgctgccc agcagcgtca acagcgagaa cggcaccagg
     2641 aacttgactg acgccgccca ccaaagccag tagcgcacgt gcgcgccgtc gcgtcgcagc
     2701 agcgccgcga cgacggcgac tgcggccagc gtcagcgtcg actgccagag atgattgccg
     2761 acctcgctca gcatggcgat cactccttcg tcgtggcttc gcgcagcgcc gcgcgggctt
     2821 cttcgatgtc ttgcagcgtc agctggcccg attcgatgag atgcgacatc agcggctgcg
     2881 cccgcccgcc gaagaggccg agcacgtcgt cgatgagctt gcgccgagca gcggtctgcg
     2941 agatcacggc ttcgaagatg tgcgcgttgc cgatcttctt cgtgcgcttc agcgcatttt
     3001 tcgcctcgag ccggtagacg atcgtctgca cggtcgtgta agccgggcgg cgcttcgccg
     3061 gaaagctctc ctggatttcg cgcaccgacg acgcgccctt ggtccagagc gcttccatga
     3121 tcttgagctc gagcttgctg agcttgggta gcggcatata cgccggtgtc ctactacgtg
     3181 tgtagtagaa tct
//
LOCUS       DATLEE010000205         6936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3098847, whole genome shotgun sequence.
ACCESSION   DATLEE010000205 DATLEE010000000
VERSION     DATLEE010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3098847"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VM692_04440"
     CDS             complement(<1..696)
                     /locus_tag="VM692_04440"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HUQ51446.1"
                     /translation="MHRIHSTRPALLVLLAALSVLLLRPAQAQRHDNERFVDAAKVLE
                     LFTTDEEHGIPTDILQRARGIAVIPNLIRGGFVFGGRRGRGVIAIRSPNGEWSNPAFV
                     TLTGGSFGAQFGAESADVVLVFANDRSVRNIASGKFTLGGDATAIAGPLGRRQQTAVT
                     GRAEVYVYMHSRGLFAGAAFEGTRLDVDQEASSAFYRNTRGVPLGAQTVETPAAALPF
                     LDALRTAAVLADTP"
     gene            complement(764..2695)
                     /locus_tag="VM692_04445"
     CDS             complement(764..2695)
                     /locus_tag="VM692_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HUQ51447.1"
                     /translation="MDFFARQEQSRRTSRALVGAFVLAFVIVAIATTIVVAAALRLYT
                     ENNALFLGTESWQQWLAGHGGLVLGVAAGTFGLMVVASLYRAATLARGGGHVARMLGA
                     TRVSGEGSDPLARRLVNVVEEIALASGLPVPEIYILEQEAGINAFAAGLTSADAAIAV
                     TRGALEQLERAELQGVIAHEFSHILNGDMRLNHQLIGLSFGILVLSLAGRWLLRSVRY
                     TGGGRRGKNSGGIAAAVAIGLALVVIGGIGVLLSRLIKAGVSRQREALADASAVQFTR
                     EPEGLAGALKKIGGYSARIVSVDTEEVAHMLFERGTRPFSGWLATHPPLMERIRALEP
                     NFDPSDLPPPKPVARTGFVATTPGAATSGLVEAAGTAPATESPLERAGEIAAPAGRAL
                     RAAVPDEVYHATRSRDSSLLVVLALALSNDEATRRQQLALVEQQLGATRAELCGRLFA
                     DLARASAELRLPVLELALPTLRERPREQLVYLSELLTRLRELEKTPRLFDFVLLRVLN
                     TYLRDLPGATPAASRRASSLATRSAVRALLANVAAYGTSDAAGARAAYAAGLASVGWR
                     AEAADPTFDPAAAARDLDLLDAALAALTSIRPRDKERVLRGVLAVIRADAVTGVEERE
                     LFRVIAIVLDCPLPPDVTL"
     gene            complement(2716..3246)
                     /locus_tag="VM692_04450"
     CDS             complement(2716..3246)
                     /locus_tag="VM692_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HUQ51448.1"
                     /translation="MYNNLVNSRNRVGNAFSQIDVQLTRRYDLIPNLVEAVKGYMGHE
                     RGTLEAVINARNAAQAGLKKAAADPSDADAIKGLAAAEATLGGTLGRLFALAEAYPDL
                     KANQNMLELQEELTSTENKVAFARQAFNDAVLSYNNACENFPGNLIANNFGFKTAQFL
                     EIEQAEKRAVPKVSFG"
     gene            3392..4333
                     /locus_tag="VM692_04455"
     CDS             3392..4333
                     /locus_tag="VM692_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HUQ51449.1"
                     /translation="MLLVAVPAQAAWVGDAIDLMGTRVSVELWADDEARGRELVADVM
                     REYHRVDDAMSTYKPDSEISQVNAHAADAPMVISDELFDLVERSLALSVASRGAFDIT
                     YDSVGYLYDFRAHLRPSDKQIEERLGAVDYRHVVLDRKQRTIFFTTHGVRINLGGIAK
                     GYVVERAGAMLRARGVEHALLNAGGDTRVIGDRLGQPWIVGIRHPRVADEVVTKLPLV
                     DEAISTSGDYERFFEENGHRYHHIINPATGKPTEGILTVTVIGPDGTMTDGLDTAIFV
                     LGVEKGLALIESYPEYETIIVDAAGKVSYSKGLTAPN"
     gene            complement(4352..5059)
                     /locus_tag="VM692_04460"
     CDS             complement(4352..5059)
                     /locus_tag="VM692_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51450.1"
                     /translation="MAIYVNKNNSFELTEPVARECPHCGTHAQLVPVANPSFQVLAQT
                     RPKHAGLLFRCAACNEPRFLRAAIRAIGAEHVELAPNLTEVERARERFQYNYLPDTVE
                     QLLRETLDCYSLGAHNAFATMCRRTTRAALRTMDADAKTRWHDTLLEVLRVCDVDAVT
                     AGSVETVLFADDGEPPEITADEAAVLVEVLKDLFYQSYVRTSKLRAAMKVRRFFAGES
                     STAKVTPIDRDGWRELA"
     gene            complement(5153..6826)
                     /gene="ettA"
                     /locus_tag="VM692_04465"
     CDS             complement(5153..6826)
                     /gene="ettA"
                     /locus_tag="VM692_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HUQ51451.1"
                     /translation="MTAQYTYVMKDLRKLVPPKREILKGIWLSFYPGAKIGVLGNNGA
                     GKSTLLKIMAGVDKDFLGEAWAAPGLKVGYLAQEPHLDPNKTVKENVDEGVAETRALL
                     QKFEDISMKLGEDMPEAKMNKLLEEQGRLQDQIDAINAWELDHQVDMAMDALRCPPGD
                     ADVTKISGGEKRRVALCRLLLSNPDMLLLDEPTNHLDAESVAWLERFLKDFPGTVIAV
                     THDRYFLDNVAGWILELDRGHGIPWQGNYSSWLEQKDERLKLEEKQEAARQRTIKTEL
                     EWVRTNPGARRAKAKARLARFEELTSKEFQTRNETNEIYIPPGPRLGDLVIEADKISK
                     SFGDRVLFENLSFSLPPGGIVGVIGPNGAGKTTLFRIMAGQDKPDNGAVRNGNSVQLA
                     YVDQSRDSLNNDKTVWDEISHGQDIMRIGTYETPSRAYVGRFNFRGTDQQKKVGLLSG
                     GERNRVHLAKLLSSGGNLLLLDEPTNDLDVETLRALEEALLNFPGSAVVISHDRWFLD
                     RIATHILAFEGNSEVVFFQGNYADYAEDLKRRKGQDADQPHRIRYKKIS"
BASE COUNT         1118 a         2261 c         2347 g         1210 t
ORIGIN      
        1 aggtgtgtcg gccagcacgg ccgcggttcg caacgcatcg aggaacggca gggcggcggc
       61 cggcgtctcg accgtctgcg cgccgagcgg tacgccacgt gtgttgcggt agaaagcgct
      121 gcttgcttcc tgatcgacgt cgaggcgcgt gccttcgaat gccgcgcctg cgaacagccc
      181 gcgcgagtgc atgtagacgt agacctcggc gcggcccgtc acggcggtct gctggcgccg
      241 cccgagtggg cccgcgatcg cggtcgcgtc gccgccgagc gtgaatttgc cgctcgcgat
      301 gttgcgcacg gaccggtcgt tcgcaaatac caacacgacg tccgcgctct cggcgccgaa
      361 ttgcgcgccg aagctgcccc cggtcagcgt cacgaacgct gggttgctcc attcgccgtt
      421 cggcgagcgg atcgcgatga cgccgcggcc gcgcctgccg ccgaacacga agccgccgcg
      481 gataaggttg ggaataacgg ctatcccgcg tgcgcgctgc aggatgtcgg tcgggatacc
      541 gtgctcctcg tcggtcgtga acagctcgag cactttggcg gcgtcgacga agcgctcgtt
      601 gtcgtgccgt tgtgcttgcg cgggccgaag caaaagcacg ctgagggccg cgagcaagac
      661 gagcagcgca gggcgagtcg aatgaatgcg gtgcatggtg cctactctcg tagaccggag
      721 tgcctgcatc ttagcgaaat acggacgacg cgccagaccg ttgctagaga gtgacgtcgg
      781 gcggcagcgg gcaatcgagc acgattgcaa tcacgcgaaa tagttcccgc tcctctacgc
      841 cggtgacggc gtcggcgcgg atcacggcaa gcacgccgcg cagcacgcgt tccttgtcgc
      901 gcggccgaat cgacgtcagc gccgcgagcg cagcatcgag cagatcgaga tcgcgcgccg
      961 ccgcggccgg atcgaacgtc ggatcggcgg cttcggcgcg ccaaccgacg gacgcaaggc
     1021 cggccgcata ggcggctcgc gcccccgcgg cgtcggaagt gccgtaggcc gcaacgttgg
     1081 ccagcagcgc gcgcaccgcg gagcgcgtgg ccaggctgct cgctctgcgc gaagccgccg
     1141 gcgtcgcgcc cggcaggtcg cgcagatagg tattgagcac gcgcagcaga acgaaatcga
     1201 acagtcgcgg cgtcttctcg agctctctga ggcgggtcag cagctcggac aaatagacca
     1261 gctgctcgcg cggccgctcg cgcagcgtcg gcagcgccag ctcgagcacc ggcaagcgca
     1321 gctccgccga cgcgcgcgcc aagtccgcga acaaacggcc gcagagctcg gctcgcgtcg
     1381 cgccaagctg ttgctcgacg agtgcgagct gttgtcggcg cgtcgcttcg tcgttcgaca
     1441 gcgcgagcgc gagcacgacg agcaggctcg agtcgcgcga gcgcgtcgcg tgataaacct
     1501 cgtcgggcac ggcagctcgc agcgcccggc ccgccggtgc cgcgatctcg ccggcccgtt
     1561 cgagcggcga ttccgtcgcc ggtgcggtgc ccgcagcttc gacgagcccc gacgtggcgg
     1621 cgccgggcgt cgtagcgacg aagcccgttc gcgcgacggg cttcggcggc ggcaaatcgc
     1681 tgggatcgaa gttcggctcg agcgcccgaa tgcgctccat gagtggcgga tgcgtagcca
     1741 gccagccgct gaacgggcgc gtgccgcgct cgaacagcat gtgcgccacc tcttcggtat
     1801 cgacggacac gatgcgcgcc gagtagccgc cgatcttttt caacgcgccg gcgagtccct
     1861 cgggctcgcg cgtgaactgc acggccgagg cgtccgccaa ggcttcgcgc tgccgcgaca
     1921 cgccggcctt gatgagcctg ctcagcagga ctccgatgcc gccgatgacg accagcgcca
     1981 ggccgatcgc gacggcggcc gcgatgccgc cgctgttctt gccgcggcgg ccgccgcccg
     2041 tgtagcggac gctgcgcagc agccagcggc ccgcgagcga caaaacgagg atgccgaacg
     2101 ataggccgat cagctggtgg ttcagccgca tgtcgccgtt caagatgtga ctgaactcgt
     2161 gtgcgatgac gccctgcagc tcggcacgtt cgagctgctc gagggcgccg cgcgtgacgg
     2221 cgatcgccgc gtcggcgctc gtgagccccg ccgcgaacgc gttgatgccg gcttcctgct
     2281 cgaggatata gatctcggga accggcagcc ccgatgcgag cgctatctcc tcgacgacgt
     2341 tgacgagccg ccgagcgagc gggtcgctgc cttcgccgct gacgcgcgtc gccccgagca
     2401 tgcgcgcgac atggccgccg ccgcgtgcca gcgtcgcggc gcggtagagg ctcgcgacga
     2461 ccatgagccc gaacgtgccg gcggcgactc cgagcacgag cccgccatgg ccggccagcc
     2521 actgctgcca gctctcggtg ccgaggaaca acgcgttgtt ctctgtatac aggcgcaacg
     2581 ccgcggctac gacgatcgtc gtcgcgatcg cgacgatcac gaatgccagc acgaacgcgc
     2641 cgacgagcgc gcgactcgtg cggcgcgact gctcctggcg cgcgaagaag tccacggctt
     2701 aactgccgcg agcgttcagc cgaaggagac cttgggtacg gcgcgcttct cggcctgctc
     2761 gatctcgagg aactgggcgg tcttgaagcc gaagttgttg gcgatcaagt tgccgggaaa
     2821 attttcgcag gcgttgttgt agctcaggac ggcgtcgttg aatgcctgac gggcaaacgc
     2881 gaccttgttc tcggtcgacg tcagctcttc ctggagctca agcatatttt ggttcgcttt
     2941 gaggtcggga taagcctcgg cgagcgcgaa caggcggccc aatgtaccgc cgagagtggc
     3001 ctcggccgcg gcaaggcctt tgatcgcgtc ggcgtcggag ggatctgccg cggctttttt
     3061 gagcccggct tgcgcggcgt tgcgggcgtt gatcacggcc tcgagagtgc ctcgctcgtg
     3121 gcccatgtaa cccttgacgg cctcgacgag attcggaatg aggtcgtagc ggcgagtaag
     3181 ctgcacgtcg atctgcgaga aagcgttacc cacgcggttg cgcgagttca cgagattgtt
     3241 gtacatcgag atcgcgtacc aaacgatggc agcgaggacc acgagggtga cgatcgttcc
     3301 cacgaatgag ctccttaccc gttcggaggc tgcgagaata cgccgccctc gctcgaggcg
     3361 ctatcgcgaa actctggcgt ttgccgcgtt tgtgctgctc gtcgcagttc ccgctcaagc
     3421 ggcatgggtc ggcgatgcca tcgatctcat gggcacgcga gtgtccgtgg agctttgggc
     3481 cgacgacgag gcgcgcggtc gcgagctcgt cgcggacgtc atgcgcgagt accaccgcgt
     3541 cgacgacgcg atgagcacgt acaagcccga cagcgagatc tcgcaggtca acgctcacgc
     3601 ggccgacgct ccgatggtca tcagcgacga gctgttcgac ctcgtcgagc ggtcgctcgc
     3661 gttgtccgtc gcaagccgcg gcgccttcga catcacttac gacagcgttg ggtacctgta
     3721 cgatttccgc gcgcatctgc ggccgtcgga taaacagatc gaagagcgcc ttggcgccgt
     3781 cgactatcgg cacgtcgtgc tcgatcgtaa gcagcggacc atcttcttca caacgcacgg
     3841 cgtccgcatc aacctcggcg gcatcgcgaa gggctacgtc gtcgagcgcg ccggcgcgat
     3901 gttacgggcg cgcggcgtcg agcacgcgct attgaacgcc ggcggcgaca cgcgcgtcat
     3961 cggcgatcgc ttgggccagc cgtggatcgt cgggatccgc catccgcgcg tcgcggacga
     4021 ggtcgtgacg aagctgccgc tcgtcgacga agcgatctcg acctcgggcg attacgagcg
     4081 gttcttcgag gagaacggtc accgctatca ccacatcatc aatccggcga cgggcaagcc
     4141 cacggaaggc attctgacgg tgaccgtcat cggacccgac ggcacgatga cggacgggct
     4201 cgacacggcg atcttcgtgc tcggcgtcga gaagggcctc gcgctgatcg agtcctatcc
     4261 cgagtacgaa acgatcatcg tcgacgcggc gggcaaagtc tcttactcga aaggtctgac
     4321 cgcgcccaat tgagcgctgc tctcggtgcg actaagcgag ctcgcgccaa ccgtcgcgat
     4381 cgatcggggt aactttggca gtcgagctct cgccggcgaa gaaacgccgc actttcattg
     4441 ccgcacgcag cttcgacgtg cggacgtagc tctgatagaa caaatcctta agcacctcga
     4501 cgagaacggc ggcttcgtcc gcagtgatct ccgggggctc gccgtcgtcg gcaaacagca
     4561 cggtttcgac gctgccggcg gtgaccgcgt cgacgtcaca aacgcgcagc acctcgagca
     4621 acgtgtcgtg ccagcgagtt ttcgcgtcgg cgtccatggt gcgcagcgcc gcgcgcgtcg
     4681 tgcgccggca catcgtcgcg aaagcgttat gcgcgccgag cgaatagcaa tcgagcgtct
     4741 cgcgcagcag ctgctcgacg gtgtcgggca gatagttgta ttgaaagcgc tcgcgcgcgc
     4801 gctcgacctc ggtcagattc ggcgcgagct cgacgtgctc tgcgccgatc gcgcgtattg
     4861 ccgcgcgaag gaagcgcggc tcgttgcacg ccgcgcagcg gaacaaaagc cccgcgtgtt
     4921 tcgggcgcgt ctgcgcgagc acttggaaag aagggtttgc gaccggtaca agttgcgcgt
     4981 gcgttccgca gtgcgggcac tcgcgcgcga ccggctccgt cagctcgaag ctgttgttct
     5041 tgtttacata aatcgccaca actgggcctt tttttggcag tgtagcagcc cggtctgcat
     5101 ttgcgacacg ccggctacgc ccgtcggccg gcgttgccgc aggtcgcggc gcttagctga
     5161 tcttcttgta gcggatgcgg tgcggctgat cggcgtcctg gcccttgcgg cgcttcagat
     5221 cctcggcgta gtcggcgtag ttaccctgga agaagacgac ctcgctgttg ccctcgaaag
     5281 cgaggatgtg cgtggcgatg cggtcgagga accagcgatc gtgcgagatc acgacggcgc
     5341 tgccggggaa gttcaagagc gcctcctcga gggcacgcaa cgtctcgacg tcgaggtcgt
     5401 tcgtcggctc gtcgagcagc agcaggttgc cgccggagga gagcagcttc gcgagatgga
     5461 cgcggttgcg ctcaccgccg gacagcaagc caactttctt ctgctgatcc gtgccgcgga
     5521 agttgaagcg accgacgtaa gcgcgcgacg gcgtctcgta ggtgccgatg cgcatgatgt
     5581 cctgaccgtg gctgatctcg tcccagaccg tcttgtcgtt gttcagcgag tcgcggctct
     5641 gatcgacgta ggcgagctgc acgctgttgc cgttgcgcac cgcgccgttg tcgggcttgt
     5701 cttgcccggc catgatgcgg aacagcgtcg tcttgccggc gccgttcggg ccgatgacgc
     5761 cgacgatgcc gcccggcggc aagctgaacg acaagttctc gaacagcacg cgatcgccga
     5821 acgacttgct gatcttgtcg gcctcgatga cgagatcgcc gagccgcggg cccggcggaa
     5881 tgtagatctc gttcgtctcg ttgcgggtct ggaattcctt cgacgtgagc tcctcgaacc
     5941 gcgcgagccg cgctttggcc ttcgcgcgcc gcgcgccggg gttcgtgcgc acccactcga
     6001 gctccgtctt gatcgtgcgc tggcgtgcgg cttcttgctt ctcctcgagc ttgagccgct
     6061 cgtccttctg ctcgagccac gagctgtagt tgccttgcca cggaatgccg tggccgcggt
     6121 cgagctcgag gatccaaccg gccacgttgt cgaggaaata gcgatcgtgc gtgacggcga
     6181 tgactgtgcc cgggaaatcc ttgaggaagc gctcgagcca cgcgacggac tccgcgtcga
     6241 gatggttcgt cggctcgtcg agcagcagca tgtcggggtt cgacagcagc aggcggcaca
     6301 gcgcgacgcg gcgcttctca ccgccggaga tcttggtgac gtcggcgtcg ccgggcgggc
     6361 agcgcagggc gtccatcgcc atgtcgacct ggtgatcgag ctcccacgcg ttgatcgcgt
     6421 cgatctgatc ctgcaggcga ccctgctcct cgagcagctt gttcatcttc gcctcgggca
     6481 tgtcttcccc gagcttcatc gagatgtctt cgaacttctg gagcagcgcg cgggtctcgg
     6541 cgacgccttc gtcgacgttc tccttgaccg tcttgttggg gtcgagatgg ggctcctgcg
     6601 cgaggtagcc gaccttgaga ccgggggcgg cccaggcttc tccgaggaag tctttgtcga
     6661 cgccggccat gatcttgagc agcgtgctct tgcccgcgcc gttgttgccg aggacgccga
     6721 tcttcgcgcc ggggtagaac gacagccaga tccccttgag gatctcgcgc ttcggcggga
     6781 ccagcttgcg cagatccttc atcacgtacg tgtactgggc ggtcatcggg ctctcctgaa
     6841 agccgcgcgg ttatacccgc cgccctcggc cttggctatt ctcggaccgt ggcgtatcgc
     6901 gacgattccg aggatgtcct ggaggcgccc accgcc
//
LOCUS       DATLEE010000206         2615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3949334, whole genome shotgun sequence.
ACCESSION   DATLEE010000206 DATLEE010000000
VERSION     DATLEE010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3949334"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VM692_04470"
     CDS             <1..1338
                     /locus_tag="VM692_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HUQ51452.1"
                     /translation="PFCQSDSGCPIDNLIPEWNDLVYRGRWREALDRLHKTNNFPEFT
                     GRACPAPCEGACVLGITDPPVTIKNIENAIIDRGFAEGWVKPQVPASRSGKRVAIVGS
                     GPAGLAAADQLNKAGHKVTVYERADRIGGLLMYGIPNMKLDKGVVNRRVDLLRAGGIE
                     FVVNADVGKNVDAAKLRAENDALLLTTGATQPRDLKIPGRELGGIHFAMDFLTANTKS
                     LLASNLTDGRNISAKDKRVVVIGGGDTGADCIGTSLRHGCKSLLNLELLDQSPPKRAA
                     DNPWPLWPKIHRSDYAHEEAIARFGADPRNFAVISKEFAGADGKVASLRINRVEWQLS
                     GGTPKMVDVEGSSELLEADLVLLAMGFLGPEAYLAKALNLELDARSNYRAAHGKFATS
                     APGVFAAGDCRRGQSLVVWAINEGRGAARAVDEFLIGTSQLPAPKLTLGGTTG"
     gene            complement(1348..>2615)
                     /locus_tag="VM692_04475"
     CDS             complement(1348..>2615)
                     /locus_tag="VM692_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ51453.1"
                     /translation="FARVGGRLRVLDRDGWVLAATGSVTPGTTGDQGLGLFGGLFRWL
                     LERDDPPSPPERPAGHVVDENLRRALRGSDTTAWYGSGPEREAIVAAAVPINGAAGVD
                     GAVVLEQASEPILTLTNRALVRLMGLTLLATLIVGAGLLGYATWLSLRVRRLAGAAQT
                     ALGPRGEIRTGMPGTGASDELGALARSFAELLERLREHNEYLRTLASKLSHELRTPLA
                     VVTTSLDNLEHEAQAPAAGEYLGRLRQGAGRLDAILAAMSEATELEQAIRETPAQPFD
                     LAGVVASCCAAYRDVYPEREIAYSQSVATATTIGSGELIAQLLDKLVDNAVSFSPPGS
                     RIDVTLAAAGDELVLTVANRGPPLPAKMRGRLFDSLVSIRERRDGRPHLGLGLHIVAL
                     VADFHGGRCEAEDLPDGSGVVFRVSLPKR"
BASE COUNT          419 a          920 c          891 g          385 t
ORIGIN      
        1 ccgttctgtc aaagcgacag cggttgtccg atcgacaacc tcattccgga gtggaatgac
       61 ctggtctatc gcggccgctg gcgcgaagcg ctcgatcgct tgcacaagac caataacttt
      121 ccggagttca ccggccgggc atgccctgct ccttgcgaag gcgcctgcgt gctcggcatc
      181 acggacccgc ccgtcacgat caaaaacatc gagaacgcga tcatcgatcg cggtttcgcc
      241 gaaggctggg tcaagccgca agtgccggcg tcgcgcagcg gcaagcgcgt cgcgatcgtc
      301 ggctcgggac ccgcgggcct cgcggccgcc gatcagctca acaaggccgg ccataaggtc
      361 accgtctatg agcgcgccga tcgaatcggc ggcctgctga tgtacggcat cccgaacatg
      421 aagctcgaca agggcgtcgt gaatcgccgc gtcgacttgc tgcgcgccgg gggtatcgag
      481 ttcgtcgtga acgccgacgt cggcaagaac gtcgacgccg cgaagctgcg cgccgagaac
      541 gatgcgctgc tcctgacgac cggcgcgacg caaccgcgcg acctcaagat ccccggtcgc
      601 gagctcggcg ggatccattt cgcgatggac ttcttgacgg ccaacacgaa gagcctgctc
      661 gcgagcaatc tgaccgacgg ccgcaacatc agcgccaagg acaagcgcgt cgtcgtcatc
      721 ggcggcgggg acacgggcgc cgactgtatc ggcacgtcgt tgcgtcacgg ctgtaagagc
      781 ttgctgaact tggagctgct ggatcagtcg ccgccgaagc gcgccgccga caacccgtgg
      841 ccgctgtggc cgaaaattca tcgctccgac tatgcgcacg aggaagcgat cgcgcgcttc
      901 ggcgccgatc cgcggaattt cgccgtcatc tcgaaggagt tcgcgggcgc cgacggcaag
      961 gtcgcgtcgc tgcgcatcaa ccgcgtcgag tggcagctgt cgggcggcac gccgaagatg
     1021 gtcgacgtcg aaggctcctc ggagctgctc gaggccgacc tcgtgctgct cgcgatgggc
     1081 tttttggggc ccgaagccta tctcgcgaaa gctttgaacc tcgagctcga cgcacgctcg
     1141 aactaccgcg ccgcgcacgg caagtttgcg acgagcgctc cgggcgtgtt cgcggccggc
     1201 gactgccgtc gcgggcagtc gctcgtcgtc tgggccatca acgaaggccg cggcgccgcg
     1261 cgcgccgtcg acgagttctt gatcggcacg agccagttgc cggcgccgaa gctgacgctc
     1321 ggcggcacga cgggctaacc cgcactctca gcgcttcggc agcgagaccc tgaagacgac
     1381 gccgctgccg tccggcagat cttcggcctc gcagcgcccg ccgtgaaagt cggcgacgag
     1441 cgcgacgatg tgcaagccga gcccgaggtg cggtcggccg tcgcgccgct cgcggatcga
     1501 cacgagcgag tcgaacagcc gcccccgcat cttcgccggc agcggcggcc cgcggtttgc
     1561 gaccgtgagc acgagctcgt cgccggccgc cgcgagcgtc acgtcgatgc ggctgccggg
     1621 cggactgaag ctcacggcgt tgtcgacgag cttgtcgagc aattgcgcaa tgagctcgcc
     1681 cgagccgatg gtcgtcgcgg tcgccacact ttgcgagtag gcgatctcgc gctcgggata
     1741 gacgtcgcga tacgcggcgc agcacgacgc gacgacgccg gccagatcga acggttgcgc
     1801 cggcgtctcg cgtatcgcct gctcgagctc cgttgcctcg ctcatcgccg cgaggatggc
     1861 gtcgaggcgc ccggcaccct gccgcagccg gcctaggtac tccccggccg cgggcgcctg
     1921 tgcctcgtgc tccaggttgt cgagcgacgt cgtcacgacg gcgagcggcg tgcgcagctc
     1981 gtgcgacagc ttgctcgcga gcgtgcgcag atactcgttg tgctcacgta gtcgctcgag
     2041 cagctcggcg aagctgcgcg cgagcgcgcc gagctcgtca ctggcgcccg tgcccggcat
     2101 gccggtgcga atctcgccgc gcgggccgag cgccgtctgc gccgcgccgg cgagccgccg
     2161 cacgcgcagc gacaaccaag tcgcgtagcc caagagcccc gcgccgacga tcagcgtcgc
     2221 gagcaacgtc aggcccatca agcgcacgag cgcgcgattc gtgagggtca gaatcggctc
     2281 gctggcctgc tcgagcacga cggcgccgtc gacgccggcc gcgccgttga tcggcacggc
     2341 ggccgcgacg atggcttcgc gctccgggcc gctgccgtac cacgccgtcg tgtcgctgcc
     2401 gcgcagcgct cggcgcaggt tctcgtcgac gacgtggccg gccggacgct cgggaggaga
     2461 aggcggatcg tctcgctcga gcagccagcg aaacaacccg ccgaacaggc ccaagccttg
     2521 gtcgccggtc gtgcccggtg tcacgctgcc ggtcgccgcc agcacccagc cgtcgcggtc
     2581 gagcacgcgc aatcgcccgc cgacgcgagc gaact
//
LOCUS       DATLEE010000207         9840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3950373, whole genome shotgun sequence.
ACCESSION   DATLEE010000207 DATLEE010000000
VERSION     DATLEE010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3950373"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VM692_04480"
     CDS             complement(<1..658)
                     /locus_tag="VM692_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51454.1"
                     /translation="MASQHESRAASPLLSAEVQQADQCDAAGAHAEAIQHLVAGTQKK
                     DVEATTRLGKRLLVGDRAPCFPKEGAGLVAEASASGGAEAAAVLAVLYAVGASQHHDL
                     RAALESLIAAAERGWAPARAQLEVLAAGVPGAENQPPPDAAAGARVLAQRVDLTAWQT
                     APPAVDLCASPLVRSYPRFASEAVCRWLVEKARGRLSRALVYEAVRREVMAKPTRTNT
                     A"
     gene            complement(752..4198)
                     /locus_tag="VM692_04485"
     CDS             complement(752..4198)
                     /locus_tag="VM692_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ51455.1"
                     /translation="MSTIKGRAVACAPRKTVSLQRKQLGLAIATALAFPAIGVHTARA
                     QNAPPDQLEQITVTGSRIVRRDYDANSPIQTIDRSSFEQQNSVALETALNELPQFVPA
                     AQGMTQLQDQSQITDNFATLTAGASTISLRGLGANRNLVLLDGYRAVPVNATMAVDVN
                     SIPAAAIERVEVITGGASSVYGADAVAGVVNFILKKDFQGLDLDVQTGSMQNGEGAES
                     RASALFGVSNADETANIMVGMELARRQPIHADDTDFWSHALRDGTTYPTQLIYTGPYF
                     TTDAANAPSRAAVDQIFNRAGAIGVVGGQGAPGRVAGGGNFFWNEDGTVYTGGASFSN
                     NTSPAGAGGTAGAYRYNGPTYTSRANANVPGDYPFRYINNEGAIVQHILPFEANIPLD
                     RTSVFGRATYDISDTIQAYAQVLNVSSDTRRFFTDSPAVAGWGNIAQHGNGVYGPSVV
                     SLGLDNLPNTGDPGENMATNPAYRPGGQYGLSCGPTGGCSKSQAFPVSPELAALLDSR
                     QDPEANWSFNYGMDFGDFGAPGNLYRSVFSETRTNQMSFGLKGEIEGIDGTWDIVASR
                     GEAKLDLRLEGYASLTRVRALLASPNWGKGFFAQGNSGAPGFGFSGGIAQCTSGVPVF
                     GDHVNISQDCLNVMYVTLNHQSKMDQKFIEGNVQGRAFMMPAGEARFSAGVHSRTNSY
                     YYIFDPLQTENSFNDNPEGFPADNTKGETSVDELYGELLLPVFGDDASRHLNLELGYR
                     YSDYELQGGVSTYKALVDWGITDTLRFRGGRQLATRAPNIAELFQSNTQSWSVQFGGE
                     PCATNSSFAYSANPAANPANAAAVRALCSQIIGTVAPTYYSAGQQAQINAFVWLPFVD
                     ATGNPQVNPEEAKTLTAGIVWSPNSDREWLNGLNLSVDYYDIKIDDMISVEGALTVYQ
                     KCLSAASNPTFSALTPSCRAIHRNPVNGTATTTTVTYVNAAFAEVSGVDLTADWSSDI
                     WGGEFGVNFMISQLLDEKTQDTVDAPVIDWKGSLGPFGTTSLNNGAFDYRTFTTLRYG
                     RDAWNLSLRWRHLPSAIDAAQAVINSNIAAGRAPANTPPSTGLGAQSSYDVFDLAGSY
                     GMGQRTTLRYGIDNLFDADAVCTGGRSAADAHPSPCGGQTEAGFYDILGRSAYVGVNV
                     SF"
     gene            4640..6160
                     /locus_tag="VM692_04490"
     CDS             4640..6160
                     /locus_tag="VM692_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HUQ51456.1"
                     /translation="MVVQSKLAAIACGLLVAAPAAAQDLCGPLTARPSFTDTTVLSAT
                     SVAAAADKTVPAYCEVQATISPVPGSKIGAVYRLPAEWNGKVLGIGGGGFAGNVRAEA
                     AAEGLARGYAVLQNDMGHPSPSALDPSFALDAQGKRNVEGIVDFGHRATHLATVVGKQ
                     IATRLYGRAPQHAYWQGCSTGGRQGLAEVQRYPDDYDGVIAGAPVYTPLTYANAMLRV
                     QMFHGRPGSNLTAAQVPLIRDAVLAACDANDGVADGILTDPRTCNWDPAELACSASSA
                     QAAECLTAAQVETVRRAYSGVRMKDGRYAAMPLMRGGESDWVARMVGTAQQPRGVNAV
                     LGAPFMANIVKADPKYDFMTFDPERDWAELDGGIAAAEVHQQNPNIAPFVARGGKLLL
                     WHGFNDPGPSPLSTIAYYDAVRERVPAAKDSTRLFLLPGVLHCAGGAGPDRFDSLTAI
                     ENWVERGVAPTSIVATKEGSPVSRPLCAYPQLPRYKGTGDTSSAASFECAAPADAR"
     gene            complement(6161..8980)
                     /locus_tag="VM692_04495"
     CDS             complement(6161..8980)
                     /locus_tag="VM692_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUQ51457.1"
                     /translation="MTELGSAGNAGNDRDPLLGRTFSHYRIVARLGGGGMGVVYKAED
                     VRLHRPVALKFITEELSGEPDALSRFTREARAASALNHSNICTVHDVGEQDGRSFIVM
                     EYLEGTPLDGRLAEGSMPLRTVLDVGIQIADALDAAHAAGIVHRDIKPANVFVGARHH
                     VKVLDFGLAQVRPPPTRGATTVEGTELGVVMGTVAYMAPEQARGERVDHRADLWSLGA
                     VLHEMATGRRPMPGVGLHVEGAPELERIIGKCLAPERELRYQHAADLRTDLASLRSGQ
                     DLTIAAPPKRARRRASWIALGAAAVVASVIAGAMIYDRRPATLTDKDTIVLAEFANTT
                     GDSVFDDTLRQGLAAQLQQSPFLSLVSDERIGRTLATMQQPRDARLTPEIAQGVCART
                     ESAAVLDGSISSLGSEYVLGLRATSCTTGDILADEQAQVARKEDVLSALSQMTTRVRE
                     RLGESHVTIEKYSRPFTEATTASLDAWKAFTTGRRVRFASGSAQAQPLFERAIAIDPE
                     FAVAHAQRVQYYADEGESALSRQSTMKAYELRHRTSEVERFYIEMGYDRQVTGNMDRE
                     MQTLEAWAQTFPRDPTPHGLMAGFVTRSTGKYDETIAAAEKQMAVDPDGGTALSHNSK
                     AYGELFLNRPDAAEATIRRAEERQLTADEFFLLRYYIAFLRDDRDGIEQHAAAAKARR
                     STEDLMSHLEGLRLAGSAQLQDAGQAAALAVAIAQQAGRLERAALFETAAAVWEALYG
                     NPTAAKAKVAAALALGRGREVDYAAAFALAMSGDVLGARELADDLARDYPEDTSVQFL
                     YLPTLRALFALDAGNAAAALESLQSATRFDLALGGIGFRGHFGKLYPIYVRGLAYLAA
                     QRPAEAAAEFKRLVDHRSIVLVDPIDALARLQLARALTLAGDTTAASSVYDDLLVRWS
                     SADADIALVAEARAQRASASAQIAR"
     gene            9193..>9840
                     /locus_tag="VM692_04500"
     CDS             9193..>9840
                     /locus_tag="VM692_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51458.1"
                     /translation="MRAIQLVISLGLLGSANTFAQTTSWGDPDLQGVWSNMTPIPLER
                     PAALADKPFLTEAEAAEAEKNALAATLKNVAGAIATSGEFNEVWLESAQGRVPRNRST
                     SLVIDPPDGRIPYTPAGRARWEETPHLTTERITGRTLRADSWEDRALQERCITSDNAF
                     YSNGFYNNYMQIVQAPGVIVIRVETMHDSRVIPLDGRPPLSTSIEQWTGDSRGRWE"
BASE COUNT         1626 a         3279 c         3250 g         1685 t
ORIGIN      
        1 cggccgtgtt cgtgcgcgtg ggcttcgcca tgacctcgcg acgcacggct tcgtagacga
       61 gcgctcgcga caaccgccca cgggccttct cgacgagcca gcggcaaacg gcctcgctcg
      121 caaatcttgg ataggagcgg acgagcggac tcgcacagag atcgacggcc ggcggcgccg
      181 tttgccacgc cgtgaggtcg acgcgttgcg ccagcacgcg cgcgccagcc gcggcgtccg
      241 gcggcggctg attctcggcg cccggtaccc ccgcggccag cacctcgagc tgcgcgcgcg
      301 ccggtgccca gccacgctcg gcggcggcaa tcaggctctc gagcgccgca cgcaggtcgt
      361 ggtgctggct cgcgccgacg gcgtacaaca cggcgagcac ggcagccgcc tccgcgccgc
      421 cgctcgcgct cgcctcggcg acgagcccgg ccccttcctt cgggaagcac ggcgcgcgat
      481 cgccgacgag caaccgtttg ccgagccgcg tcgtcgcttc gacgtccttc ttctgcgtgc
      541 cggcgacgag atgctggatc gcctcggcgt gcgcgccggc ggcatcgcat tggtccgctt
      601 gctgcacttc cgcggagagc agcggacttg cggcgcgcga ttcgtgttgc gatgccatgg
      661 tgtgccaagc accttagcct gccgcacgct cgaaaggaaa cggcgagccc gagggctcgc
      721 cgtttaccgc aacgtgcgcg aagaggcgcg cttagaacga tacgttcaca ccaacgtagg
      781 cgcttcgacc caggatgtcg tagaagcccg cctccgtctg gccaccgcac gggctcggat
      841 gcgcgtccgc ggcgctgcgg ccacccgtgc agacggcgtc cgcgtcgaac aggttgtcga
      901 tgccatagcg cagcgtcgtc cgctgaccca tgccgtaact gccggccaga tcgaacacgt
      961 cgtacgagct ctgagcaccg agccccgtgg acggcggcgt gttcgccggg gcgcggccgg
     1021 ccgcgatgtt ggagttgatg acggcctgcg cggcgtcgat cgcgctaggc agatggcgcc
     1081 aacgcagcga caagttccag gcgtcgcgtc cgtacctgag cgtcgtgaag gtccggtagt
     1141 cgaatgcacc gttgttcaac gacgtggtgc cgaatgggcc aagactgccc ttccagtcga
     1201 tgacgggtgc gtccacggta tcttgcgtct tctcgtcgag cagctgcgag atcatgaagt
     1261 tcacgccgaa ttcgccgccc cagatgtcgc tcgaccagtc ggccgtgagg tcgacgcccg
     1321 agacttccgc gaacgccgcg ttcacgtagg tcacggtcgt ggtcgtggcg gttccgttca
     1381 cggggttgcg atggatcgcg cggcacgacg gagtcagagc gctgaacgtc gggttcgagg
     1441 ccgcgctgag acacttctga tacaccgtga gcgcaccttc gaccgagatc atgtcgtcga
     1501 tcttgatgtc gtagtagtcg acgctgaggt tgagcccgtt cagccactcg cggtccgagt
     1561 tcgggctcca gacgatgccg gccgtcagcg tctttgcttc ctcggggttc acttgcggat
     1621 tgcccgtcgc atcgacgaac ggcaaccaga cgaacgcatt gatctgagct tgctggccag
     1681 ccgagtagta ggtcggcgcc acggtcccga tgatctgcga gcacaacgcc cgcacggcgg
     1741 cggcgttcgc cggattggcg gcgggattcg cgctgtaggc gaagctgctg ttggtcgcgc
     1801 acggctcgcc gccgaactgc accgaccacg attgcgtgtt cgattggaac agctccgcga
     1861 tgttcggcgc tcgcgttgcc agctggcggc cgccgcggaa acgcagcgtg tcggtgatgc
     1921 cccagtcgac caacgccttg tacgtgctga ccccgccctg cagctcgtag tcggagtagc
     1981 gatacccgag ctcgagattc aggtgtcgcg aggcatcgtc gccgaacacc ggcaacaaca
     2041 attcgccgta gagctcgtcg acggaggtct cgcccttcgt gttgtcggcc gggaacccct
     2101 ccgggttgtc gttgaacgag ttctccgtct gcagcggatc gaagatgtag tagtacgagt
     2161 tcgtgcgcga gtgaacaccc gcggagaagc gagcttcgcc ggcgggcatc atgaaggcgc
     2221 gcccctgcac gttaccttcg atgaactttt gatccatctt cgactgatga ttcagcgtca
     2281 cgtacatgac gttgaggcag tcctgactga tgttcacgtg atcgccgaac accggcaccc
     2341 cgctcgtgca ctgcgcgatg ccgcccgaga agccgaagcc gggagccccg gagttgcctt
     2401 gcgcgaagaa tcctttgccc cagttcggcg aggccagcaa cgcgcgaacg cgcgtcagcg
     2461 acgcgtagcc ctcgaggcga agatcgagct tcgcctcgcc gcgcgacgcg acgatgtccc
     2521 aggtgccgtc gatgccctcg atttcaccct tgaggccgaa cgacatctgg ttcgttcgcg
     2581 tttcgctgaa caccgagcga tacaaattgc ccggggcacc gaagtcgccg aagtccatgc
     2641 cgtagttgaa gctccagttc gcttccgggt cctgacgcga gtcgagcagc gccgcgagct
     2701 ccggcgagac cggaaatgcc tgggacttcg agcaaccgcc cgtgggcccg cagctcaagc
     2761 cgtattgacc gccggggcga tacgccgggt tcgtcgccat attctcgccc ggatcgcccg
     2821 tgttcggcag attgtccagg ccgagagaga ccaccgacgg cccgtaaacc ccgttgccgt
     2881 gctgcgcgat gttgccccag cccgcgaccg ccggagaatc ggtgaaaaag cggcgcgtgt
     2941 ccgaggagac gttcaacacc tgcgcgtacg cttgaatcgt gtcgctgatg tcgtaggtgg
     3001 cgcggccgaa caccgaggtg cgatcgagcg gaatgttcgc ttcgaacggc aagatgtgct
     3061 gaacgatcgc gccttcgttg ttgatgtagc ggaacgggta atcgcccgga acgttcgcgt
     3121 tcgctcgtga cgtgtacgtc gggccgttgt agcggtaagc accggccgtg ccacccgctc
     3181 ctgccggcga cgtgttgttg ctgaacgagg cgccgcccgt atagacggtg ccgtcctcgt
     3241 tccagaagaa attcccgccg ccggcgactc gaccgggcgc accttgtccg cccacgactc
     3301 cgatcgcacc ggcgcggttg aagatctgat cgaccgcggc acggctcggc gcattggccg
     3361 cgtcggtcgt gaagtacggg cccgtataga tgagctgcgt cgggtacgtc gtcccgtcgc
     3421 gcaaagcgtg cgaccagaaa tcggtgtcgt cggcatgaat cggctggcgc cgcgcgagct
     3481 ccatgccgac catgatgttg gccgtctcgt ccgcgttcga gacgccgaac aacgccgacg
     3541 cacgcgactc ggcgccttcg ccgttttgca tcgagcccgt ctgaacgtcg aggtcgaggc
     3601 cctggaagtc cttcttcaaa atgaagttca cgacaccggc gacggcgtcg gcgccgtaca
     3661 ccgacgatgc gccgcccgtg atgacctcga cgcgctcgat cgcggccgcg ggaatcgagt
     3721 tcacgtcgac ggccatcgtc gcattgaccg gcacggctcg ataaccgtcg agcagcacga
     3781 ggtttcggtt cgcgccaaga ccgcgcaggc tgatcgtcga cgcgccggcc gtcagcgtcg
     3841 cgaagttgtc ggtaatctgg ctctggtcct gcagctgcgt catgccctga gccgccggga
     3901 cgaactgcgg cagctcgttg agcgccgtct cgagcgcgac ggagttctgc tgctcgaacg
     3961 agctcctgtc gatcgtttgg atcgggctgt tggcgtcata gtcgcgacgg acgatgcgcg
     4021 agcccgtgac ggtgatttgc tcgagttgat ccggcggcgc gttttgcgcc cgcgcggtat
     4081 gcacgcctat cgcgggaaat gccagcgccg tggcgatcgc caggccaagt tgcttgcgtt
     4141 gcagagaaac cgttttgcgt ggcgcgcacg caacggcgcg acccttgatc gtcgacataa
     4201 tggacccccg attgtgagtg caatcaaacg cgcagacgaa acgatcttgc atcgatgcct
     4261 cgctccggga caggtaagcg aagttgctat tgcagacgct acccgatcgc gatcggttgc
     4321 gtcgcgtcga cgccacgcgg catgcaactt gcaactcgag gtggggacgt catgctttgg
     4381 ggacagcatg catagcaatg acagtgctct cggcaactcg atcgttcaga agcagcccga
     4441 caccatcgtc tacacagagc tcgaaacgca gcttcttaaa gccgttgcgg actttggtgt
     4501 ggacgagctg agtcaactga tcgacgcggc cacagctctg cgacgcgcgc gacgcctgta
     4561 atcggcggcg caggtcggcg gcggtggcga gccgcggtcg tgccatactc acgcggcgtc
     4621 acgacgctgc gggggaatca tggtcgttca atcgaagttg gccgcgatcg catgcggcct
     4681 gctcgttgcg gcgcccgcgg cagcgcaaga cttatgcggc ccgctgacgg cgcgcccgtc
     4741 gttcaccgac acgacggtcc tgtccgcaac gtcggttgcc gccgcagcgg acaagaccgt
     4801 ccccgcgtac tgcgaggtgc aggcgacgat ctcgccggtg cccggctcga aaatcggcgc
     4861 cgtctatcgc ctgccggccg agtggaacgg caaagtgctc gggatcggcg gtggcggctt
     4921 cgcgggcaac gtgcgtgccg aagcggcggc cgagggattg gcgcgcggct atgccgtgct
     4981 gcaaaacgac atgggtcatc cgagcccgag cgcgctcgac ccgtcgttcg cgctcgatgc
     5041 gcaaggcaaa cgcaacgtcg aaggcatcgt cgatttcggc caccgcgcga cgcatcttgc
     5101 gacggtcgtc ggcaagcaga tcgcgacgcg gctctacggc cgcgcgccgc agcacgcgta
     5161 ttggcagggc tgctcgacgg gcggccgcca aggcctcgcc gaggtgcagc gttaccccga
     5221 cgactacgac ggcgtgatcg cgggcgcgcc cgtctacacg ccgctgacgt atgcgaacgc
     5281 catgttgcgc gtgcagatgt ttcacggccg gcccggcagc aatctgacgg cggcacaggt
     5341 gccgttgatc cgcgacgccg tgctcgccgc gtgcgatgcg aacgatggcg ttgccgacgg
     5401 gattctcacc gacccgcgca cctgcaactg ggacccggcg gagctcgcgt gctcggccag
     5461 ctcggcacaa gcagcggagt gcctcacggc cgcgcaggtc gagaccgtgc gccgcgccta
     5521 ttccggagtg cgcatgaagg acggccgcta cgccgcgatg ccgctgatgc gcggcggcga
     5581 gagcgactgg gtcgcgcgca tggtcgggac ggcgcagcag ccgcgcggcg tgaacgccgt
     5641 gctgggggcg ccgttcatgg cgaacatcgt gaaggccgat ccgaagtacg acttcatgac
     5701 cttcgatccc gagcgcgact gggccgagct cgacggcggc atcgccgcgg ccgaggtgca
     5761 ccagcagaac ccgaacatcg cgccgttcgt cgcgcgcggc ggcaagctgc tgctgtggca
     5821 cggcttcaac gatcctggcc cgagcccgct gtcgacgatc gcgtactacg acgccgtgcg
     5881 cgagcgcgtg ccggccgcga aggattcgac gcggctgttc ttgctgcccg gcgtgctgca
     5941 ctgcgcgggc ggcgcggggc ccgaccgctt cgattcgttg acggccatcg agaactgggt
     6001 cgagcgcggc gtggcgccga cgtcgatcgt cgcgacgaag gaaggctcgc cggtgtcgcg
     6061 cccgctctgc gcgtatccgc agctgccgcg ctacaaaggc acgggcgaca cgagctcggc
     6121 ggcgagcttc gagtgcgccg cgccggccga cgcgcgctag ctagcgggct atctgcgcgc
     6181 tcgcgctcgc acgttgcgcg cgcgcttcgg caacgagcgc gatgtccgca tcggcactac
     6241 tccaccgcac cagcaggtcg tcgtaaacgc tactcgccgc ggtcgtgtcg ccggcaagcg
     6301 taagggctcg ggcgagctgt aaccgcgcca gcgcatcgat cgggtcgacg agcacgatgc
     6361 tgcgatggtc gacgagccgc ttgaactcgg cggcggcctc ggccggccgt tgcgccgcga
     6421 gataggcgag gccgcgcacg tagatcggat acagcttgcc gaagtgccct ctgaatccga
     6481 tcccgccgag agcgaggtcg aaacgagttg cactctgcag cgattcgaga gccgctgcag
     6541 cattacctgc gtccagcgcg aacagcgccc gcagcgtcgg cagatacagg aactgcaccg
     6601 acgtatcctc aggataatcg cgggccagat cgtcggcgag ctcgcgcgcc ccaagcacat
     6661 cgcccgacat ggctagcgcg aacgcggccg cataatcgac ttcgcgcccc ctaccgagcg
     6721 cgagcgccgc ggcgaccttc gcttttgctg ccgtcgggtt gccgtacaac gcttcccaca
     6781 cggccgctgc ggtttcgaac aacgcagccc gctcgagtct gccggcctgc tgcgcgatcg
     6841 ccaccgcgag cgccgctgct tgccccgcgt cttgaagttg cgcggagccg gcgagacgca
     6901 agccttcgag gtgcgacatc aagtcttccg tcgaccgccg cgctttcgcg gccgcggcgt
     6961 gctgctctat gccatcgcga tcatcacgca ggaatgcgat gtagtagcgg agcaagaaaa
     7021 actcatcagc ggtcagttgg cgctcttccg ctcgacgaat ggtcgcctcg gccgcgtcgg
     7081 gcctgtttag aaagagctcg ccgtacgctt tactgttgtg cgaaagcgcc gtgccaccgt
     7141 cgggatcaac cgccatctgt ttttcggccg cggcgatcgt ctcatcgtat ttgccggtgc
     7201 tgcgcgtaac gaagccggcc atcaatccgt gaggagtcgg atcgcgtgga aacgtctgcg
     7261 cccacgcttc caatgtttgc atctcgcggt ccatgttgcc ggtcacttgc cggtcgtacc
     7321 ccatctcgat gtagaaccgc tcgacctcgc tcgtgcgatg gcgcaactcg tacgccttca
     7381 tcgtgctctg ccgcgacagt gccgactcgc cctcatcggc gtagtactgc acacgttgtg
     7441 catgagcgac cgcgaactcc ggatcgatgg cgatcgcacg ctcgaacagc ggctgagcct
     7501 gcgcgctgcc ggatgcgaaa cgcactcgcc tcccggtggt gaacgccttc cacgcgtcca
     7561 gcgacgccgt cgtcgcttcg gtgaacggtc tcgagtactt ctcgatggtg acgtgcgatt
     7621 cgcccagtcg ctcgcgtacc cgagtggtca tctggctcag cgcgctgagg acgtcctcct
     7681 tgcgcgcgac ctgcgcttgc tcgtcggcga ggatgtcgcc tgtcgtacaa ctcgtcgcgc
     7741 gcaagcccag cacgtactca ctgcccaagc tgctgatcga tccatcgagg acggccgcgc
     7801 tttccgttcg cgcacataca ccctgagcga tttccggagt aagccgtgcg tcgcgcggct
     7861 gctgcatcgt cgccagcgtt ctgccgatgc gctcgtccga aacgaggctc aagaacggcg
     7921 attgctgcag ttgtgccgcg agcccttggc gcagcgtgtc gtcgaacacc gaatcgccgg
     7981 tcgtgttcgc aaactcggcg agcacgatcg tgtccttgtc ggtcagcgtg gcggggcggc
     8041 ggtcatagat catcgccccc gcgatcacgg acgcgacgac ggctgccgcg ccgagcgcaa
     8101 tccacgaggc gcggcgacgc gcacgcttcg gaggggccgc gatcgtcaag tcttgcccgc
     8161 ttctgaggct cgcgagatcg gtccgcaggt cggccgcgtg ctgatagcgg agctcgcgtt
     8221 ccggagccag gcatttgccg atgatgcgct cgagctccgg cgcgccctcg acgtgcaagc
     8281 cgacacccgg catgggccgt cggccggtcg ccatctcgtg aaggacggcg ccgagactcc
     8341 agaggtcggc acgatggtcg acgcgctcgc cgcgggcctg ctcgggagcc atgtacgcga
     8401 cggttcccat cacgacgcca agctcagtcc cttcgaccgt cgtcgcgcct cgcgtcggcg
     8461 gcggtcgcac ctgggcgagc ccgaaatcga gcactttcac gtggtggcgc gcgcccacga
     8521 agacgttggc gggcttgatg tcgcgatgga cgatgccggc cgcatgcgcg gcgtcgagcg
     8581 cgtcggcgat ctggataccc acgtcgagca cggtgcgaag cggcatcgaa ccttcggcga
     8641 gcctgccgtc gagcggcgtg ccctcgaggt actccatgac gatgaatgag cgcccgtcct
     8701 gctcgcccac gtcgtggacc gtgcatatgt tcgagtggtt cagcgcggac gccgcccgcg
     8761 cttcgcgcgt gaatcggctc aatgcgtcgg gctcgcctga gagctcttcg gtgatgaact
     8821 tgagcgccac ggggcggtga agccgcacgt cttcagcctt gtagacgacg cccatgccgc
     8881 cgccgcccag cctagcgacg atgcggtagt gtgagaatgt ccgaccgagc agcggatctc
     8941 gatcgttacc cgcgttcccc gcacttccga gttccgtcac gtcacccgac atagcgcttt
     9001 tcggtcaagc acgcttgcgg cgggcggcaa agaaatcgag tgggcggcga ttcacgagat
     9061 cccctcggcc ttgcgtctgt gcgcgtcgag aggataccgc agacgcacgt gcttcggacg
     9121 ctggtcgtcg cggctcagcc cgaccttgcc acgggttacc tcaagcggga cactttgcgc
     9181 aacggagaag ccatgcgcgc gattcaactc gtgatctcct tgggcctgct ggggtctgcg
     9241 aacaccttcg ctcagacgac gtcttggggc gaccctgacc ttcagggcgt gtggtcgaac
     9301 atgacgccga ttcccctcga acgcccggcc gcgctcgcgg acaagccgtt cctcaccgaa
     9361 gccgaagcgg ccgaagcaga gaaaaacgcc ctcgccgcta cgctcaagaa cgtcgccggg
     9421 gcgatcgcca cgagcggaga gttcaacgag gtctggctcg agtcggctca aggtcgagtg
     9481 ccgcgcaatc gcagcacgtc gctcgtgatc gatccgccgg acggcagaat tccctacaca
     9541 ccggcaggca gagctcgctg ggaagagacg ccgcacctga cgacggaacg aatcacggga
     9601 cgcacgctgc gcgcggacag ttgggaggat cgcgcgcttc aggagcgctg catcacctcc
     9661 gacaatgcgt tctattcgaa cgggttctac aacaactaca tgcagatcgt gcaagcgcca
     9721 ggcgtgatcg tcatccgtgt cgagaccatg cacgactcgc gcgtgatccc gctcgatggc
     9781 cgcccgccgt tgagcacgag catcgagcag tggaccggcg attcacgcgg ccggtgggaa
//
LOCUS       DATLEE010000208         2354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1718744, whole genome shotgun sequence.
ACCESSION   DATLEE010000208 DATLEE010000000
VERSION     DATLEE010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718744"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VM692_04505"
     CDS             complement(<1..610)
                     /locus_tag="VM692_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ51459.1"
                     /translation="MRATQFLRALLALVVLAAAGLAAAQQRTLPPLKIEQLTPHVYRY
                     GGLTNGAFVVGSEGIAVIDGQICGSNGTQWLKDELAKRFPGVPVKYTVLSHDHEMHIC
                     GLEVFSDTAIAISHVNAKGHIIREKRRTVIPQVTFDERMELDLGGIEVVLFYLGPTHT
                     DNLIQVHIPAEKVLIAVDFVREGKSLAMPELRDLNLDNSIRAL"
     gene            complement(658..2154)
                     /locus_tag="VM692_04510"
     CDS             complement(658..2154)
                     /locus_tag="VM692_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ51460.1"
                     /translation="MLKAAQRDDAAVALAAIEDGVDVNGKGPDGTTALHWAVYHDNVA
                     LVQRLIAAGADVRAANEFGSTPLAEAATVGNAVVIDALLDAGAEVDAQGADGQTALMV
                     VARSGNTAAAKALLDHGADVNARERWREQTALMWAAAQNQPAMTKLLIERGADVDARS
                     FVNDWPRQVTGEPRRMYRPFGGLTPLLFAAREGCRECARALIEAGAKLDLPDPKGVTA
                     LFLAIDNFHFDTAKLLIEAGANPNKWDWWGRTALYSAVDVNTLPHGGRADRRSVDTTT
                     STEIIELLLEAGAWPNVQLKLLPPYRHIGDDRGCDTMLVTGTTPLLRAAKTFDTDAIE
                     LLIDHGAKVDLPNDGGITPVMAAAGVGSVECDARGYGPGIPHYMAPDTEQRSIAALKL
                     LLDADANINARTVSGRAGARSAGRTALFGAAFWGWNDVVDYLVERGAQIDVAERGGLT
                     PVDAAMGKAGGHGRGSTIEVYEDTAARLRELCEQQKDCDLAKPDGPAS"
     gene            complement(2132..>2354)
                     /locus_tag="VM692_04515"
     CDS             complement(2132..>2354)
                     /locus_tag="VM692_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51461.1"
                     /translation="RWRASLRASAPENPLQRHLGRSAVFSSRKTFLFSYEIFFFSCKT
                     LLFSSVRIRIALLRRRRARGGGFAQGGAA"
BASE COUNT          362 a          787 c          802 g          403 t
ORIGIN      
        1 cgagcgcgcg gatcgagttg tcgaggttca ggtcgcgcag ctccggcatc gcgagactct
       61 tgccttcgcg tacgaagtcg acggcgatca gcactttctc ggccggaatg tgcacctgga
      121 tcaagttgtc cgtgtgcgtc ggccccaaat agaacaacac gacctcgatg ccgccgaggt
      181 cgagctccat gcgctcgtcg aacgtgacct gcgggatcac ggtgcggcgc ttctcacgga
      241 tgatgtggcc cttggcgttc acgtgcgaga ttgcgatcgc cgtgtcgctg aacacctcga
      301 gcccgcaaat gtgcatctcg tgatcgtggc tcagcaccgt gtacttgacc ggaacgccgg
      361 ggaagcgctt cgcgagctcg tccttgagcc actgcgtgcc gttgctgccg cagatctgcc
      421 cgtcgatgac ggcgattccc tcggagccga cgacgaacgc gccgttcgtg agaccgccgt
      481 agcgatagac gtgcggtgtc agctgctcga tcttcagcgg cggcagcgtg cgttgctgcg
      541 cggcggcgag gccggccgcg gctaggacga cgagcgcgag caaagctcgc aggaattgcg
      601 ttgcgcgcat ggaacccccc ccgaattagt agttcaagcc gtaatttcta ggtttcgtta
      661 cgacgccggg ccgtcgggtt tcgcgaggtc gcagtccttc tgttgctcgc aaagctcgcg
      721 cagtcgcgcc gcggtgtctt cgtacacttc gatcgtcgag ccgcggccgt ggccgccggc
      781 tttgcccatg gccgcgtcga cgggcgtcag ccccccgcgc tcggcgacgt cgatttgcgc
      841 gccgcgctcg acgaggtaat cgacgacgtc gttccagccc caaaacgcgg cgccaaacag
      901 cgccgtgcgc ccggcactgc gcgcgccggc gcgtcccgac accgtacgcg cattgatatt
      961 ggcgtcggcg tcgagcagca gcttcagcgc ggcgatcgac ctctgctccg tatcgggcgc
     1021 catgtagtgc ggaatgccgg ggccgtagcc gcgagcgtcg cattcgacgg agccgactcc
     1081 ggccgccgcc atcacgggcg tgatgccgcc gtcgttcggc aggtcgacct tggcgccgtg
     1141 gtcgatcagc agctcgatcg cgtccgtgtc gaaggtcttg gcggcgcgca gcagaggcgt
     1201 cgtgcccgtg acgagcatcg tgtcgcagcc gcggtcgtcg ccgatgtgcc ggtagggcgg
     1261 cagcaacttg agctgcacgt tcggccaggc gccggcctcg agcaacagct cgatgatctc
     1321 cgtgctcgtc gtcgtatcga cggaccgacg gtcggcgcgg ccgccgtgcg gcagcgtgtt
     1381 gacgtcgacg gccgagtaga gcgccgtgcg gccccaccag tcccacttgt tcggattcgc
     1441 gccggcttcg atgagcagct ttgccgtgtc gaaatggaag ttgtcgatcg cgaggaagag
     1501 ggccgtcacg cctttcgggt cgggcaggtc gagcttcgct ccggcttcga tgagcgcgcg
     1561 tgcacattcg cggcagcctt cgcgcgcggc gaacaagagc ggcgtcaggc cgccgaacgg
     1621 ccggtacatg cggcgcggct cgcccgtgac ttgccgcggc caatcgttga cgaaggaacg
     1681 cgcgtcgacg tcggcgccgc gctcgatgag cagcttcgtc atcgccggtt ggttttgcgc
     1741 cgccgcccac atcagcgcag tctgttcgcg ccagcgctcg cgcgcgttga cgtcggcgcc
     1801 gtggtccagc agcgctttgg ccgcggccgt gttgccgctg cgcgcgacga ccatgagcgc
     1861 cgtctggccg tccgcgccct gcgcgtcgac ctccgcgccc gcatcgagca gcgcgtcgat
     1921 gacgaccgcg ttgccgaccg tcgcggcttc cgcgagcggc gtcgagccga attcgtttgc
     1981 ggcgcgcacg tcggcaccgg ccgcgatcag ccgctgcacg agcgcgacgt tgtcgtggta
     2041 gacggcccag tgcagcgccg tcgtgccgtc cggcccttta ccgttgacgt cgactccatc
     2101 ctcgatcgcc gccagcgcca cggccgcatc gtcacgctgc gccgccttga gcaaatcctc
     2161 cgccgcgtgc gcgccgacgg cgcagaagag caatgcgaat gcgaacagag gaaaagagaa
     2221 gagtcttgca cgaaaagaaa aagatctcgt acgagaagag aaaggtcttg cgcgacgaaa
     2281 aaaccgctga ccgcccgaga tggcgttgca gagggttctc cggggcgctg gcgcgcaggg
     2341 acgcgcgcca gcga
//
LOCUS       DATLEE010000209         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3952906, whole genome shotgun sequence.
ACCESSION   DATLEE010000209 DATLEE010000000
VERSION     DATLEE010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3952906"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VM692_04520"
     CDS             complement(<1..210)
                     /locus_tag="VM692_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51462.1"
                     /translation="MSKSMLVGVVAGVGLATAGGVLGYQFFNETPNEQGSNVVVETPE
                     ETVVARVEPAAPPPAPQAVVQPAAQA"
     gene            complement(325..>2065)
                     /locus_tag="VM692_04525"
     CDS             complement(325..>2065)
                     /locus_tag="VM692_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HUQ51463.1"
                     /translation="DERSALAGLTFRETVGGTPRKPIHRYEFPPQEHDVRRGDEVCVP
                     VTGKPVGKIVGVDVAAHTVDVEHSGRYADVRPERMFVRRMVSPKPKPSVLLEIGRWLA
                     ANDVDADGPHRAARDLLLKRPPRLVAGSQGLAAVEGAPALSDSRVIAFAAARSARERQ
                     RAAAVAPPSDVDAEVKEAIRLAFELDHGVLPIQGPPGTGKTFTGSHMILELLKAGKKV
                     GVTAVGHETIRNVLRAVCERAAEQGLADFQCLHKGKPKDESPEALHAFDDNDRIAQLF
                     ASDDYWLLGGTSWLWANERFRDSVDVLFIDEAGQMSLADVLAVSAGAKSLVLLGDPQQ
                     LEQPQQASHPPGAGASALEHLLGGTKTIASDRGLFLHQTRRLHPDICAFTAEAFYEGR
                     LKSVAGLERQAVLAPPGTSAATLGSAGLVFVPVEHDANQSRAKEEVDTIAELVEALTA
                     HGVQYLSADGEHAPLRRADLMIVAPYNAQVTALAERLPDVRIGTVDKFQGQQAPVVIV
                     SLTTSTPEDAPRGMDFLYSANRFNVATSRAKALCILVGNPQLFEPDCRTPQQMRLANA
                     FCRYRELAREVSV"
BASE COUNT          309 a          703 c          709 g          344 t
ORIGIN      
        1 cgcttgcgcc gccggctgaa cgacggcttg cggcgccgga ggcggcgcgg cgggctcgac
       61 gcgcgccacg acggtctcct cgggcgtttc gacgacgacg ttgctgcctt gctcgttcgg
      121 cgtttcgttg aagaactgat agccgaggac gccgcccgcc gttgcaaggc cgacacccgc
      181 gaccacaccg accaacatcg acttgctcat gtgcgaatcc ctccgtggaa tgacgggcac
      241 tttgccgggc cacgcgggct cgcttccgtg acgcgggtca cgggcgcgcg tttgtcacag
      301 caactcaacg taatgccgag cgcgttagac cgagacctcg cgcgcgagct cgcgataacg
      361 gcagaacgcg ttcgcgagcc gcatttgttg cggtgtgcgg caatcgggct cgaagagctg
      421 cggattgccg acgagtatgc agagcgcctt ggcgcgtgaa gttgcgacgt tgaagcggtt
      481 tgcgctgtac aaaaagtcca tgccgcgcgg cgcgtcctcg ggcgtcgacg tcgtcaacga
      541 gacgatgacg acgggcgctt gctggccctg gaatttgtcg acggtgccga tgcgtacatc
      601 gggcaaacgc tccgcgagcg ccgtgacctg cgcgttgtaa ggcgcgacga tcatgaggtc
      661 ggcgcgccgc agcggcgcgt gctcgccgtc cgcgctcaag tactgcacgc cgtgcgcagt
      721 gagcgcttcg acgagctccg cgatggtgtc gacttcctct ttcgcgcgcg actggttggc
      781 gtcgtgctcg acgggcacga agacgaggcc cgcgctgcca agcgtggcag ccgacgtccc
      841 cggtggcgca agcacggctt gccgctcgag acccgctacc gacttcaagc gtccctcgta
      901 gaacgcctcc gccgtgaacg cacagatgtc ggggtgcagg cgacgcgtct ggtgcaggaa
      961 caggccgcga tcgctcgcta tcgtcttggt gccgccgagc aagtgctcga gcgcggacgc
     1021 cccggcgccc ggcggatggc tcgcttgctg cggctgctcg agctgctgcg gatcgccgag
     1081 cagcacgaga cttttcgcgc cggccgaaac ggcgagcacg tcggccagcg acatttggcc
     1141 ggcttcatcg atgaacagca cgtcgacgct gtcgcggaag cgctcgttcg cccatagcca
     1201 cgacgtgccg ccgagcagcc agtagtcgtc gctcgcgaac agctgcgcga tgcggtcgtt
     1261 gtcgtcgaat gcgtgcaacg cctcggggct ctcgtccttc ggtttgcctt tgtgcaggca
     1321 ttggaagtcc gcaagccctt gctcggccgc gcgctcgcaa acggcgcgca gcacgttgcg
     1381 aatcgtctcg tggccgacgg ccgtgacgcc gaccttctta ccggccttca agagctcgag
     1441 gatcatgtgc gagcccgtga acgtcttgcc ggtgcctggc gggccctgga tcggcagcac
     1501 gccgtggtcg agctcgaacg cgagccggat cgcttccttg acctcggcgt cgacgtccga
     1561 tggcggcgca acggcggcgg ctcgctgtcg ttcgcgcgcg ctgcgcgccg ccgcgaacgc
     1621 gatgactcgc gagtcggata gcgcaggcgc cccttcgacc gccgcgagac cttgcgagcc
     1681 cgcgacgagc cgcggcggcc gcttcagcag caggtcacgc gccgcgcggt gcggcccgtc
     1741 agcgtcgacg tcattcgccg cgagccagcg gccgatctcg agaagaacgc tcggcttcgg
     1801 cttcggcgac accatgcgcc gcacgaacat gcgctcgggc cgcacgtcgg cgtaacggcc
     1861 cgaatgctcg acgtcgacag tgtgtgccgc gacgtctacg cctacgatct tgccgacggg
     1921 cttgcccgtg acgggcacgc aaacttcgtc gccgcggcgc acgtcgtgct cttgcggcgg
     1981 aaactcgtag cgatggatgg gcttgcgcgg cgtgccgccg accgtctcgc ggaacgtcag
     2041 ccctgcgagc gccgagcgct cgtcc
//
LOCUS       DATLEE010000210         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10551143, whole genome shotgun sequence.
ACCESSION   DATLEE010000210 DATLEE010000000
VERSION     DATLEE010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10551143"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VM692_04530"
     CDS             <1..626
                     /locus_tag="VM692_04530"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010494606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HUQ51464.1"
                     /translation="RGLSPHRQSRPAAVNLRGRKPRSEKLMFVIDSRTALRVLREDDA
                     AELFALTDANRVHLRRWLPWVDLVSSEDDSRSFLETMSAQREDGRGPTFGVVCDGALA
                     GVVGFLPIDRVNRCGEIGYWLAEREQGRGLMTQSCRFVVRYGFLTLDLHRVQIAAGTE
                     NTASRAIAERLGFRLEGILRGRENLYGTFIDHAMYSLLRSEFDEGFA"
     gene            736..1482
                     /locus_tag="VM692_04535"
     CDS             736..1482
                     /locus_tag="VM692_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HUQ51465.1"
                     /translation="MAVIAVGLAAWPQLGARRDAAASFEVVAAYPHDPNAFTQGLAIE
                     AGRLYEGTGQYGASTVRRVDLQSGRPEKQRALNQRYFGEGIAILGGLLYQITWQNNVV
                     VVYDLETFEVERTMQYDGEGWGLTHDGRELIMSDGSATIRFRDPKTFAVTREIEVRAD
                     GAPLRQLNELEYIDGEIWANIRYDDRIARISPANGAVLGFIDLATLYPQSARTSEAVL
                     NGIAYDAAAKRLFVTGKNWPQLYEIKVARP"
     gene            1556..>2016
                     /locus_tag="VM692_04540"
     CDS             1556..>2016
                     /locus_tag="VM692_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HUQ51466.1"
                     /translation="MAKSPLGQIFARKTVEQMHAEHEHGELKRSLGAMNLVLLGIGCI
                     IGTGIFVLTGRAAAQFAGPGIMISFIITGTLCALVALAYAELASAIPVSGSAYSYSYA
                     SLGELAAWIMGVLLVLEYGIAASTVAVGWSGYVASFLHDLGLGIPTALRGAP"
BASE COUNT          318 a          666 c          679 g          353 t
ORIGIN      
        1 gtcgtggact atcaccgcat cgtcagtccc gaccagcggc agtgaacctg cgcggccgca
       61 agccgcgcag cgagaagctg atgttcgtca tcgattcaag gacggccttg cgcgtgctgc
      121 gcgaggacga tgccgcggag ctgttcgcgc tcacggatgc caatcgcgtt cacttgcgcc
      181 ggtggctgcc atgggtcgat ctcgtgtcgt ccgaagacga ctcccgctcg ttcctcgaga
      241 cgatgagcgc gcagcgcgag gacggccgcg gtcccacgtt cggcgtggtc tgcgacggcg
      301 cgctggccgg cgtcgtgggg ttcttgccga tcgaccgcgt caatcgttgc ggcgagatcg
      361 gctactggct cgccgagcgc gagcaaggcc gcggcctcat gacgcagagc tgccgtttcg
      421 tggtccgcta cggcttcctg acgctggatt tgcaccgcgt gcagattgcc gccgggaccg
      481 agaacacggc gagccgcgcg atagccgagc gccttggctt caggctcgag ggcatcctgc
      541 gcgggcgcga aaatctctac gggaccttca tcgaccacgc gatgtattcg ctgctgcgca
      601 gcgagttcga cgaaggtttc gcctagctgc gcttgctggc cccgcggcac tttgccaagc
      661 tagcccgcta tgtcagtcac tgcagctcct cccgcacgcg gtcggcgatg ggtcgtctgg
      721 accttggttc tcgccgtggc cgttatcgcg gtcgggctcg cggcgtggcc gcagctcggt
      781 gcacgacgcg acgcggcagc gagcttcgag gtcgtcgccg cgtatccgca cgatccgaat
      841 gcgttcacgc agggcctcgc gatcgaggcc ggccgcctct acgagggcac gggccaatac
      901 ggcgcgtcga cggtgcgccg cgtagacctg cagagcggcc gacccgagaa gcagcgcgcg
      961 ctgaaccagc gctatttcgg cgagggcatc gcgatcctgg gcggcctgct gtaccagatc
     1021 acttggcaga acaacgtcgt cgtcgtgtac gacctcgaga cgttcgaagt tgagcgcacg
     1081 atgcaatacg acggcgaggg ctgggggctc acgcacgacg gccgcgagct catcatgagc
     1141 gacggctccg ccacgattcg tttccgcgat cccaagacat ttgccgtgac gcgcgagatc
     1201 gaagtccgcg ccgacggtgc gccgctgcgg caattgaacg agctcgagta catcgacggc
     1261 gagatctggg cgaacattcg gtacgacgat cgcatcgcac gcatctcgcc ggcgaacggc
     1321 gcggtgctcg gattcatcga tctcgccacg ctgtatccgc agagcgcgcg cacgagcgaa
     1381 gcggtgttga acggcatcgc ctacgacgcc gccgcgaagc gcctgttcgt cacgggcaag
     1441 aactggccgc aactctacga gatcaaggtc gctcgaccgt gaccctcggc cgcggcctgg
     1501 tgtagagtcc gaggcagcac cggtccccgt ggagcggctt caaggagttc gttcgatggc
     1561 caagagtccc ctcgggcaga tcttcgcgcg taagacggtc gagcagatgc acgccgagca
     1621 cgagcacggc gagctcaaga ggtcgctggg cgcgatgaac ctcgtgctgc tcggcatcgg
     1681 ttgcatcatc ggcaccggca tcttcgtgct gacgggacgc gcggccgcgc agttcgcggg
     1741 gccgggcatc atgatctcgt tcatcatcac gggcacgcta tgcgcgctcg tcgcgctggc
     1801 gtatgccgag ctcgcctcgg cgataccggt atcgggctcc gcctattctt attcgtacgc
     1861 gtcgctcggc gagctcgccg cgtggatcat gggcgtgttg ctcgtgctcg agtacggaat
     1921 cgccgcgtcc acggtcgccg tcggctggtc cgggtacgtc gcaagcttcc tgcacgacct
     1981 cggcttgggc atacccaccg cgctgcgcgg cgcccc
//
LOCUS       DATLEE010000211         3522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_980941, whole genome shotgun sequence.
ACCESSION   DATLEE010000211 DATLEE010000000
VERSION     DATLEE010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980941"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VM692_04545"
     CDS             <1..1572
                     /locus_tag="VM692_04545"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HUQ51467.1"
                     /translation="KVYATGETTTDAEPSLDVFYKVTPSLNAALTINTDFSATEIDDR
                     QVNLTRFSLFFPEKRDFFLQDADIFEFGRIGSGGFQGGGRGGGGGGGGGGGGGGGGGG
                     NPAIPGAAVQNARPFFSRKLGLSSTGQLIDIEHGAKLSGRVGRWNLGALAIKQDDFGA
                     IEGDDTLVARVAANVLGESALGVIMTDGDPQTNNDSSLAGADFRYRNSRLPGGRLLEG
                     SAWYQETDNTGLNLRTGSAFTDDDNRAFGMGLSMPNSIGWRGGFSTRQVENNFYPAVG
                     FVDRIGIRDQALDFGRQWRFVGKPLRSFYTGLDNYRVTKLDTGNEESKVVSLRVTAQN
                     NTQDALFFRAVVNREVLLAPFLIYAAPNDPTVRVLIASGDYEFTDYRLGIDSGDQRKI
                     ALRASLTSGEYYDGDHTNKSAEVTWRPSPKLRFGVNYDVHSIDLPAGDFIVRQSTLRA
                     ELVFSSTLSWVNLIQYDNVSENLGLNSRLHWIPQAGREGFIVLNRNLTDLDRDDSFTP
                     LTADVAVKFSYPWRF"
     gene            complement(1586..>3522)
                     /locus_tag="VM692_04550"
     CDS             complement(1586..>3522)
                     /locus_tag="VM692_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ51468.1"
                     /translation="FDDAGAAMLDVERFIAKYPEIERVSWYDTAGQALLSLNGDGVTT
                     TPVTALDTAATTDLAARAGVNPPYLLTQNVSPGQRYRLSGPIWTETFADDALFSEDPA
                     AEETTVRLLGFVAVDLDFSAYESALWPPLAVASGLLLLLLGVSWAFGRWFLKSALAPL
                     SDLETSLAQLAAEEQTVHFPSSPYTELRTIVAALEDTMQALQRRERRLLHLANHDQLT
                     GLANRHRFVAELSAELVRCANEGARGALFFIDLDQFKYVNDTCGHAAGDHLLQLAAQQ
                     LRYAVRAEDFLARFGGDEFVVLVRNVSRNEARAAAGKVLELMRGLKHVERDQVFHLQC
                     SIGITLLQGTRTSAHEVIAQADIACQSAKTHGRNRVEIYSAAGKQSEQIAKDVVTMNR
                     LRGALETDRFELLYQPLMHIKSGVVSHYETLLRLPTEEGVLGPDSFLPAAFRFGLMGE
                     IDRWVLKRVVRSLAELSATMPDVKLSTNLSGFAFEDEGLGAYLRDLLKEHNVDGDRLV
                     LEITEQLAVRFAANTDRQIALLRDLGCQLAIDDFGSGYSSFSYLKRLPVDYLKIDGAF
                     IKSLARDRVDQAMVRMVGEVARAAGIETVAEYVHSAATLELLAKYGIDYAQGYFIGKP
                     TAAPEPSRVTLVTARASRAS"
BASE COUNT          602 a         1172 c         1162 g          586 t
ORIGIN      
        1 aaggtctacg cgacgggcga gaccacgacg gacgcggagc cctcgctcga cgtcttctac
       61 aaggtcacgc cgtcgctgaa cgcggcgctg acgatcaaca cggatttctc ggcgacggag
      121 atcgacgacc ggcaggtcaa cttgacgcgc ttcagcctgt tcttccccga gaagcgcgat
      181 ttcttcctac aggacgccga catcttcgag ttcggccgca tcggcagcgg cggctttcag
      241 ggcggcggcc gaggtggcgg cggcggtggg ggtggcggcg gaggcggcgg cgggggaggc
      301 ggcggcaatc cggcgatccc cggcgcggcc gtgcaaaacg cgcggccatt cttctcgcgc
      361 aagctcggct tgagctcgac gggccagctc atcgacatcg agcacggcgc gaagctctcg
      421 ggccgcgtcg gccgctggaa cctcggcgcg ttggcgatca agcaggacga cttcggcgcc
      481 atcgaaggcg acgacacgct cgtcgcgcgc gtggccgcga acgtgcttgg cgaatcggcg
      541 ctcggcgtca tcatgaccga cggcgacccg caaacgaaca acgacagcag cctcgcaggg
      601 gccgactttc gttatcgcaa ctcgcgcttg cccggcggcc ggctgctgga aggctcggcg
      661 tggtatcagg aaacggacaa cacggggctc aacctgcgca cgggctcggc gttcacggac
      721 gacgacaatc gagccttcgg catggggctc agcatgccga acagcatcgg ctggcgcggc
      781 ggcttcagca cgcggcaggt cgagaacaat ttttatcccg ccgtcggctt cgtcgaccgc
      841 atcggcatcc gcgaccaggc gctcgatttc gggcgtcagt ggcgtttcgt cggcaagccg
      901 ctgcgctcct tttacacggg cctcgacaac tatcgcgtga cgaagctcga caccggcaac
      961 gaggagtcga aggtcgtctc gttgcgcgtg acggcccaga acaataccca ggacgcgctg
     1021 ttcttcaggg ccgtcgtgaa ccgcgaggtg ctgctcgcgc cgttcctgat ctacgcggcc
     1081 ccgaacgatc cgaccgttcg cgtgctgatt gcgtccggcg actacgagtt cacggattac
     1141 cggctcggca tcgattcggg cgaccagcgc aagatcgcgt tgcgagcttc gctgacttcg
     1201 ggcgaatact acgacggcga ccatacgaac aagagcgccg aggtgacgtg gcggccgtcg
     1261 ccgaagctgc gtttcggagt caactacgac gttcactcga tcgacttgcc ggccggcgat
     1321 ttcatcgtgc ggcaatcgac gctacgcgca gagctcgttt tctcgtccac gttgtcctgg
     1381 gtgaatctga ttcagtacga caacgtgtcc gagaacttag gcctcaacag ccgcctgcac
     1441 tggatcccgc aagccggccg cgaaggcttc atcgtgttga accgcaacct cacggatctc
     1501 gaccgcgatg actcgttcac gccgctgacg gccgacgtgg ccgtcaagtt cagctacccc
     1561 tggcgcttct gagactcgcg cgccgtcagg acgcgcggct cgcgcgtgcg gtcacgagcg
     1621 tgacgcgcga cggctcgggc gcagccgtcg gcttgccgat gaagtaaccc tgcgcgtaat
     1681 cgatgccgta cttcgcgagc agctcgagcg tggcggcgct gtgcacgtac tccgcgacgg
     1741 tctcgatgcc ggccgcgcgc gcgacctcgc cgaccatccg caccatggcc tggtcgaccc
     1801 gatccctggc caagctcttg atgaaagcgc cgtcgatctt caagtagtcg acgggcagcc
     1861 gcttcaggta gctgaaggag ctgtagccgc tgccgaaatc gtcgatcgca agctggcaac
     1921 caagatcgcg cagcaacgcg atctggcgat cggtgttggc ggcgaaccgc accgcgagct
     1981 gctcggtgat ctcgagcacg agccggtcgc cgtccacgtt gtgctctttt agtaggtcgc
     2041 gaagatacgc gccgagccct tcgtcctcga acgcgaaccc cgagaggttc gtcgacagct
     2101 tgacgtccgg catggtggcc gaaagctccg cgagcgaccg cacgacgcgc ttcaagaccc
     2161 agcgatcgat ctcgcccatg agcccgaagc gaaacgccgc cggcaggaac gagtcgggac
     2221 cgaggacgcc ttcctcggtc ggcaagcgca acagcgtctc gtagtgcgag acgacgccgg
     2281 acttgatgtg catcagcggc tggtacagca gctcgaaccg gtcggtttca agtgcgccgc
     2341 gcaggcgatt catcgtgacg acgtctttcg cgatctgctc gctctgcttg ccggccgcgc
     2401 tgtagatctc gacgcggttc cggccgtgcg tcttcgcgct ctggcaggcg atgtcggcct
     2461 gcgcgatgac ctcgtgcgcg ctcgtgcggg tcccttgcag cagtgtgatg ccgatgctgc
     2521 attgcaaatg gaacacctga tcgcgctcga cgtgcttcag cccgcgcatc agctcgagca
     2581 ctttgccggc ggcggcacgc gcctcgttgc gcgacacgtt gcgcacgagc acgacgaact
     2641 cgtcgccgcc gaagcgcgcc aagaagtcct cggctcgcac ggcgtaacgc agctgctgcg
     2701 ccgcgagctg cagcaagtgg tcgccggccg cgtggccgca cgtgtcgttg acgtacttga
     2761 attgatcgag gtcgatgaag aacagcgctc cgcgcgcgcc ttcgttggcg caacgcacga
     2821 gctcggcgct gagctccgcg acgaagcgat gccgattcgc gagccccgtc agctggtcgt
     2881 gattcgcgag gtgcagcaac cggcgttcgc gccgctgcag tgcctgcatc gtgtcctcga
     2941 gcgcggcgac aatggtccgc agctcggtat acggcgagct cgggaagtga accgtttgct
     3001 cctcggccgc gagctgcgcc agcgacgtct cgagatccga cagcggcgcg agcgcgctct
     3061 tcaagaacca gcggccgaac gcccaagaca ctccgagcag cagcaacagc aggccgctcg
     3121 cgacggccag cggcggccac agagcgctct cgtaggccga gaagtcgagg tcgacggcga
     3181 cgaagccgag cagccgcacc gtcgtttcct cggccgccgg gtcctcgctg aacaacgcgt
     3241 cgtcggcgaa ggtttccgtc cagatcgggc ccgacaaccg gtagcgctgc cccggcgaga
     3301 cgttctgcgt caggaggtac ggcgggttga cgccggcacg cgcggcaagg tccgtcgtcg
     3361 ccgccgtgtc gagcgcagtg accggcgtcg tggtcacgcc gtcgccgttc agcgagagca
     3421 gcgcctgccc tgcggtgtcg taccagctga cgcgctcgat ctcggggtac ttcgcaatga
     3481 agcgctctac gtcgagcatc gccgcgccgg catcgtcgaa at
//
LOCUS       DATLEE010000212         2774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6826318, whole genome shotgun sequence.
ACCESSION   DATLEE010000212 DATLEE010000000
VERSION     DATLEE010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6826318"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..915
                     /locus_tag="VM692_04555"
     CDS             76..915
                     /locus_tag="VM692_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HUQ51469.1"
                     /translation="MRMARSEFLSWPHKAITLLGMSGVGKTTLAYKLPQSKWFHYSGD
                     YRIGTKYLAEPILDNIKRQAMQVDFLRDLLRNDSIYIASNVTVHNLQPVSTFLGKIGR
                     ADLGGLPVAEFKRRQRLHREAEIGAMRDVAEFIVKAQEIYGYDHLINDAGGSLVELDD
                     ERTEKTLAEHTLILYLKADDDMERELVRRAVSDPKPLYYNEKFLDKKLVEYVSEAELG
                     GPDEIVPDEFVRWIFPALVAHRRPKYEAMADRYGYTLNARLAEAITSERDFLELVASA
                     LDK"
     gene            complement(923..1870)
                     /locus_tag="VM692_04560"
     CDS             complement(923..1870)
                     /locus_tag="VM692_04560"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUQ51470.1"
                     /translation="MADAADEGRDARGRATQGAVAEQWVTVATRLTEVRVSELALVLT
                     ARGVPFQRQPDPRGGWELWVPLAMAPVAATELTLYKHENARQIGSRPLDEVGAGKAGV
                     AWYVATLLAVFFALHTDLFNRDWLAAGRLEAGPLLNGEWWRAVTALTLHIELDHLGGN
                     LAFGAFFGYFVGRYLGHGVGWLAVLLAASGANVLNAWVQSPLHRSIGASTAVFAALGL
                     LVAYTWRRGFLRDTPWRARIAPIVAGLGLLAFTGTAGENTDLGAHMFGFIAGFAAGGL
                     LGGFAPTAWLKSKRVQRVCGAVAALMLIAAWAVGLRTAG"
     gene            1972..2493
                     /locus_tag="VM692_04565"
     CDS             1972..2493
                     /locus_tag="VM692_04565"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HUQ51471.1"
                     /translation="MGSTAPIITTSARRSATAYCYWDLVPSPFRSLCVAVDGDGRLVE
                     IRFDSRRPEGRRDAKRCQDASLQLADYFAGRRREFDLELAPFGTDFQLRVWNALREIP
                     FGTVRNYGDVARAIGQSGAARAVGQANGCNPLPIVIPCHRVIASDGTIGGYSGGLAIK
                     HRLLALENIELDL"
     gene            2496..>2774
                     /locus_tag="VM692_04570"
     CDS             2496..>2774
                     /locus_tag="VM692_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51472.1"
                     /translation="MGHDLRCYRCGEPLAKLSLPLTRLDECPKCRVHLHVCRMCERYA
                     PRKPKGCSEDDAIEVRDKKGANFCDYFKPNARAFNPAEQQADDAARSAL"
BASE COUNT          494 a          986 c          878 g          416 t
ORIGIN      
        1 gcgcaacgca gctgagcggg gcttacggcc gcgccgctat aatcgccgcc agtcatctgc
       61 aggactaaac cgcgcatgcg catggctcgc tccgagttcc tgtcctggcc gcacaaggcc
      121 atcaccctgc tcggcatgtc cggcgtaggc aagacgacgc tcgcttacaa gctgccgcag
      181 tcgaagtggt ttcactactc cggcgactat cggatcggca cgaagtacct cgcggagccg
      241 atcctcgaca acatcaagcg ccaggcgatg caggtcgact tcctgcgcga cctgctgcgc
      301 aacgactcga tctacatcgc gagcaacgtc acggtccaca acctgcagcc ggtgtcgacg
      361 ttcctcggca agatcggccg cgcagatctc ggcggcttgc cggtggccga gttcaagcgc
      421 cggcaacgcc tgcaccgcga ggccgagatc ggcgcgatgc gcgacgtcgc cgagttcatc
      481 gtcaaagcgc aggaaatcta cggctacgac cacttgatca atgacgccgg cggcagcttg
      541 gtcgagctcg acgacgagcg gaccgaaaag acgctcgccg agcacacgtt aattctctat
      601 ctaaaggccg acgacgacat ggagcgcgag ctcgtgcgcc gcgccgtcag cgacccgaag
      661 ccgctctatt acaacgagaa gtttctcgac aagaagctcg tcgagtacgt ctcggaggcc
      721 gagctcggcg ggcccgacga gatcgtgccc gacgaattcg tgcgctggat ctttcccgcc
      781 ctcgtcgcgc accgtcggcc gaagtacgaa gcgatggcgg accgttacgg ttacacgctg
      841 aacgcgcggc tcgccgaagc gatcacgagc gagcgtgatt ttctcgagct cgtcgcgagc
      901 gcgctcgaca aatagcctga actcaacccg ccgttcggag gccgacggcc cacgccgcga
      961 tcagcatgag cgccgcgacg gcgccgcaga cgcgttgcac gcgcttgctc ttgagccacg
     1021 cggtcggcgc gaagccgccg agcaggccgc cggccgcgaa ccccgcgatg aagccgaaca
     1081 tgtgcgcgcc gaggtccgtg ttctcaccgg ccgtgcccgt gaaggccaac agccctagcc
     1141 ccgcgacgat cggcgcgatg cgcgcacgcc acggcgtgtc gcgcaggaag ccgcgccgcc
     1201 acgtgtaggc cacgagcaac ccgagcgccg cgaacacggc cgtcgaggcg ccgatcgacc
     1261 gatgcaacgg cgactgcacc caggcgttca gcacgttggc gccgctcgcc gcgagcagca
     1321 ccgcgagcca gccgacgccg tgaccgagat agcggccgac gaagtagccg aagaacgcgc
     1381 cgaacgcgag gttgccgccg agatggtcga gctcgatgtg cagcgtcaat gcggtgacgg
     1441 cgcgccacca ttcgccgttg agcagcggcc ccgcttcgag ccggcccgcg gcgagccagt
     1501 cgcgattgaa tagatccgtg tgcagcgcga agaacacggc cagcaacgtc gcgacatacc
     1561 aggccacccc cgccttgccc gcgccgactt catcgagcgg ccgcgagccg atttgccggg
     1621 cgttctcgtg cttgtaaagc gtgagctccg tggccgcgac gggcgccatg gcgagcggca
     1681 cccacagctc ccagccgcca cggggatcgg gctgccgctg aaacggcacg ccacgcgccg
     1741 tcaaaacgag cgccagctcg ctgactcgca cctccgtgag gcgcgtcgcc accgtaaccc
     1801 actgctcggc aactgctcct tgcgttgctc tacctcgggc atccctgccc tcgtccgcgg
     1861 cgtcggccat ctctgctagc atccggtgct aaataaaatg cgcccccttc cgggacgcca
     1921 aaggcgcgcc gcgggcgagg caccgccaag cggcacgcta aggtagcaga cttgggcagt
     1981 acggcaccca tcatcacgac gtccgcgcgc cgctcggcga cggcctattg ctactgggat
     2041 ctcgtgccct cgccgtttcg ctcgctgtgc gttgccgtcg acggcgacgg tcggctcgtc
     2101 gagattcggt tcgacagccg ccggcccgaa ggccggcgtg atgcgaagcg ctgtcaggat
     2161 gcttctctgc agctcgccga ctatttcgcg ggccggcgcc gcgagttcga cctcgagctc
     2221 gcgccgttcg gcacggattt tcagctgcgc gtctggaacg cgctgcgcga gattccgttc
     2281 ggcaccgtgc gcaactacgg cgatgtcgcg cgagcgatcg gtcagtccgg cgccgctcgc
     2341 gccgtcggcc aggccaatgg ctgcaacccg ctgccgatcg tgatcccgtg ccaccgcgtc
     2401 atcgcgagcg acggcacgat cggcggctac tcgggcggtc tcgcgatcaa gcatcgcttg
     2461 ctcgcgctcg agaacatcga gctcgatctc tagcgatggg ccacgacctt cgctgttacc
     2521 gctgcggcga gccgctcgcc aagttgtcgc tgccgcttac gcgcctcgac gaatgcccga
     2581 agtgccgcgt gcatctgcat gtctgccgca tgtgcgagcg gtacgcgccg cgcaagccga
     2641 aaggctgtag cgaggacgac gcgatcgagg tccgcgacaa aaaaggcgca aatttctgcg
     2701 actacttcaa gccgaacgcc cgcgcattca accccgccga gcagcaagcc gacgacgccg
     2761 cgcgctcggc gctc
//
LOCUS       DATLEE010000213         2451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9175709, whole genome shotgun sequence.
ACCESSION   DATLEE010000213 DATLEE010000000
VERSION     DATLEE010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9175709"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /gene="pgi"
                     /locus_tag="VM692_04575"
     CDS             <1..1112
                     /gene="pgi"
                     /locus_tag="VM692_04575"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HUQ51473.1"
                     /translation="HALAEDVRRGLNVHFVSNVDGVALAHVLQRVNAETTLFVICSKT
                     FTTLETLVNAQAAREWLLAAGGKPAVAANCVAVSTNEAAMNEFGIAADRRLAMWDWVG
                     GRYSVWSAVGLSVALAVGWPKFAEFLAGGAALDQHFRTAPLERNLPVLLALLGVWNRN
                     LLDAATHAVLPYDDHLVRFPAYLQQLEMESNGKSVRRGGEPVECATCPVIWGEPGSNA
                     QHSFYQLLHQGTERVSIDFLLPARSSVDRQQQQDLASANCLAQAWALAVGDPSEKARG
                     PHQRYPGSRPSSLLLFERLDAATLGKLVALYEHKVFVQGVIWDINSFDQWGVQLGKRL
                     ASELTPFVAGDAGAARPPAALTGALAALRALHHGR"
     gene            1347..>2451
                     /locus_tag="VM692_04580"
     CDS             1347..>2451
                     /locus_tag="VM692_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HUQ51474.1"
                     /translation="MAQTANPGPTPEQLEIFRNLPPDQQRAILDEVAGGNGSLRSEDP
                     LATPRVTAPADRFPRPEPEGPPRMERGQAVLLEVDIADGVSDQDVLRLLTNRRDRIRG
                     SNPYSLDADGRLSLPFLAPIPLAGLTKEEAERRLSSDPRLAGLEFEVTMLQLARTGSE
                     ALKPFGYDVFRESPTTFAPATDIPVPPDYAIGPGDTVIVELFGEQSGRYSLVVDRLGQ
                     LNLPDFGPLQVAGRNFDSMRAEIERRVTEETIGVRASVSMGPLRSIRVFVLGDVARPG
                     SYTVSGLSTITHALFVSGGVSEVGSLRNVELKRDGNTVTGLDVYDLLLRGDTSNDERL
                     QSSDVVFVPPVGTIASIGGRVRRPAIYELKSETS"
BASE COUNT          389 a          817 c          807 g          438 t
ORIGIN      
        1 tgcacgcgct cgccgaagac gtgcggcgcg gcttgaacgt ccatttcgtc tcgaacgtcg
       61 acggcgtggc gctcgcgcac gtattgcagc gcgtcaatgc ggaaacgacg ttgttcgtca
      121 tctgttcgaa gacctttacg acgctcgaaa ctctcgtcaa cgcgcaggcc gcgcgcgagt
      181 ggttgctcgc ggccggcggc aaacccgctg tcgcggcgaa ctgcgtcgcc gtctcgacga
      241 acgaggccgc gatgaacgag ttcggcatcg cggccgatcg acgcctcgcc atgtgggatt
      301 gggtcggcgg ccgctattcg gtctggtcag ccgtgggctt gagcgtcgcg ctcgcggttg
      361 gctggcccaa gttcgcggag ttcctggccg gcggcgcggc gctggaccag catttccgca
      421 ctgcgccgct cgagcgcaat cttccggtcc tgctcgcgct gctcggcgtc tggaatcgca
      481 atctcttgga cgcggcgact cacgccgtgc tgccgtacga tgaccattta gtgcgctttc
      541 cggcgtatct gcagcaactc gagatggaga gcaacggcaa gtccgtgcgg cgcggcggcg
      601 agcccgtcga atgcgcgacc tgcccggtga tctggggcga gccgggctcg aatgcgcagc
      661 actcctttta tcagctgctg caccaaggca cggagcgcgt ctcgatcgat ttcttgttgc
      721 cggcgcgctc gagcgtcgac cgtcagcagc agcaagacct cgcgtccgcc aattgccttg
      781 cgcaggcttg ggcactcgcc gtcggtgatc ccagcgaaaa ggcgcgcggc ccccatcagc
      841 gttatcccgg cagccgaccg agctcgctgt tgctgttcga gcggctcgat gcggcgacgc
      901 tcggcaagct cgtcgcgctg tacgagcaca aggtgttcgt gcaaggcgtg atctgggaca
      961 tcaattcgtt cgaccaatgg ggcgtgcagc tcggcaaacg gctcgcgtcg gagctcacgc
     1021 cgttcgtcgc aggtgacgcc ggcgcggcgc ggccgcccgc cgctttgaca ggcgcgcttg
     1081 cagcgctgcg agctttgcac cacggccgct gaagcgatcg cactcgtcaa gctgcggcgc
     1141 ggtgcacgca cgtcgcgctt cgcgcaacaa cttctccgca gcagcatgcg aatcgggttg
     1201 ccccagtctt atctgctacg tatgatccgc acgtccccgt aggaccgggt ttatccagaa
     1261 tgcgaatgcc acgagcagcg cgcacccgcg cgccgttggg cgcactcata atttcaatcg
     1321 tcgccgtact gctgccccat tggggtatgg cccagacggc gaatcccgga cctacacccg
     1381 aacagctcga gatcttccgc aaccttccgc ccgatcagca acgtgcgatt ctcgacgagg
     1441 tcgccggggg taacggcagt ctgcgatccg aggatccgtt ggcgacgccg cgagtgaccg
     1501 ctccggcgga tcgcttcccg cgcccggagc cggaaggtcc gccgcgcatg gagcgcggcc
     1561 aggcagtgct actcgaagta gacatcgccg acggtgtttc ggatcaggac gtcttgcgac
     1621 tgctgacgaa ccgtcgcgat cggattcgag gcagtaatcc gtacagcttg gacgccgatg
     1681 gccgactgag cctgccgttt ctggcgccga tcccgctcgc cggcctcacg aaggaggagg
     1741 ccgaacgacg cctgagctcg gacccgcgtc tcgccgggct cgagttcgaa gtcacgatgc
     1801 tgcagctggc gcggacaggc tccgaagcgc tcaagccgtt tggctacgat gtcttcaggg
     1861 aatcgccgac gacgtttgcg cccgccaccg atatccccgt tccccccgac tatgcgattg
     1921 gcccgggcga caccgtcatc gtcgagttgt tcggcgagca gtcgggccgc tactcgctcg
     1981 tcgtggatcg gctgggccaa ctgaacctgc ccgactttgg gccgctgcag gtcgcgggcc
     2041 ggaatttcga cagcatgcgg gccgaaatcg agcgccgcgt gaccgaggaa accatcggcg
     2101 ttcgcgcgag cgtgagcatg ggtccgctgc gctcgatccg cgtcttcgtg ctcggcgacg
     2161 tcgcgcgtcc ggggtcctac acggtcagcg gcctgtcgac gatcacgcac gcgctgttcg
     2221 tcagcggtgg cgtgtcggaa gtgggctcgc tgcgcaacgt cgagctcaag cgcgacggca
     2281 ataccgtcac cgggctcgac gtctacgacc tcctgctgcg tggcgacacg tcgaacgacg
     2341 agcgcctgca gtccagcgac gtagtgttcg tgccgcccgt cggcacgatc gcgtcgatag
     2401 gcggccgggt acgccggccg gctatctacg agctcaagtc ggaaacgagc g
//
LOCUS       DATLEE010000214         3116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6827112, whole genome shotgun sequence.
ACCESSION   DATLEE010000214 DATLEE010000000
VERSION     DATLEE010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6827112"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VM692_04585"
     CDS             <1..937
                     /locus_tag="VM692_04585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ51475.1"
                     /translation="EPWLRVPCDYRDPQRRGGAYEVYWCAESRFGQVMPRPSAAEVAE
                     SYEVTGYYTHENGAPAPARLSLANRVLQHLAWRADAGAHIDAAALKPFVDRGGSRICD
                     IGCGAGTLLEAAIAAGFTTAVGIESDPSARAAAARKGLAVYAGTAEQLPLEAQARRFD
                     LVLMSHVLEHTLSPVQALRNAAGLLEPGGSMMIEVPNNEACGLDFAGNCWCWLDVPRH
                     LNFFTTTSLRSICRLVGLEPRNMEYAGYSRQFSVEWQANQAYIHSIFASVPGGGGAAK
                     PSPLSYWHLLARTVFATPERKYDSVRAWAQKVHDQ"
     gene            951..2018
                     /gene="rfbB"
                     /locus_tag="VM692_04590"
     CDS             951..2018
                     /gene="rfbB"
                     /locus_tag="VM692_04590"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HUQ51476.1"
                     /translation="MKVLVTGGAGFIGSAVVRHLLAQPSVHVVNVDKLTYAGNLESLT
                     SVSGSPRYAFVRGDICDLSMLSRVFAEHRPDAVMNLAAESHVDRSIDSSDMFLQTNLV
                     GTGRLLQAARAYWSGLSAEQRQAFRFLHVSTDEVFGDLGEGGAPCRETAAYAPSSPYA
                     ATKAGSDHLVRAWSRTYELPVLLTNCSNNYGPYQFPEKLIPHTILRALRGASLPVYGD
                     GRHVRDWLYVDDHARALYTVLTKGRSGETYNIGGNGEQRNIEVVRQLCHILNAALPSQ
                     RPPRGFESLIAFVTDRPGHDRRYAIDAHKIEKELHWRPAETFASGLEKTVQWYLENRG
                     WWQRVLDGSYGLERAGLKVTE"
     gene            2015..2890
                     /gene="rfbA"
                     /locus_tag="VM692_04595"
     CDS             2015..2890
                     /gene="rfbA"
                     /locus_tag="VM692_04595"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HUQ51477.1"
                     /translation="MTRRGIVLAGGAGTRLHPVTLAVCKQLLPIYDKPMIYYPLSTLM
                     LAGIRTVLIISTPQDTALFEQLLGDGARLGMDISYAVQPHPGGLAQAFLIGERFVRAE
                     PSALILGDNLFFGSGLQGLVREAAADIEGATVFAYRVATPEAFGVVGFDEAGRVTSIE
                     EKPAIPKSNYAVTGLYFYDARVVEIAKSLKPSARGELEITDINRAYLDRGTLKVRRMA
                     RGMAWLDTGTHDSLLEAAHFMQTLEKRQGLKVGCPEEVAWRLGWIDDGQLMKLAEPFA
                     DRAYGRYLLALLEQA"
BASE COUNT          550 a          989 c         1009 g          568 t
ORIGIN      
        1 ggagccgtgg ctgcgcgttc cctgcgacta tcgagatccg caacgacgtg gcggcgccta
       61 cgaggtctac tggtgcgccg aaagccggtt cgggcaggtc atgccgcggc cgtcggcggc
      121 cgaggtcgca gagtcttacg aggtcacggg gtactacaca cacgagaacg gcgctccggc
      181 gcccgcgcgc ctttcgctcg ccaaccgagt gcttcagcac cttgcctggc gagcggatgc
      241 cggcgcgcac atcgacgcgg cggcgctcaa acccttcgtc gatcgcggtg gctcgagaat
      301 ctgcgacatc ggctgcgggg cgggaacgct tctcgaagcg gcgatcgctg cgggcttcac
      361 gaccgccgtc ggcatcgagt ccgacccgtc ggcgcgtgcc gctgccgcac gcaaagggct
      421 ggccgtatac gcaggaaccg ccgaacagtt gccgttggaa gcgcaagctc gccgtttcga
      481 cctggtcttg atgagccacg tgctcgagca tacgttgagc cccgtgcaag cgctgcgcaa
      541 cgccgcgggg ctgctcgaac cgggtggaag catgatgata gaggtaccga acaacgaggc
      601 ttgcgggctc gacttcgcag gcaattgttg gtgttggctc gacgttcctc gtcacctgaa
      661 tttcttcacg acgacgagcc tccgctcgat ctgccggctc gtcgggctcg agccgcgcaa
      721 catggagtac gccggttaca gtcggcagtt ttccgtcgag tggcaagcca accaagcgta
      781 catccacagc atcttcgcct cggtcccggg gggcggcggc gcggcgaagc cgagccctct
      841 ttcgtattgg cacttgctgg cgcgaaccgt cttcgcgaca cccgaacgaa agtacgactc
      901 cgtccgggcc tgggcgcaga aggtgcatga ccagtaacgg aactcaacct ttgaaggtac
      961 tggtgaccgg cggcgccgga ttcatcggtt ccgctgtcgt ccgccatctg ctcgcgcagc
     1021 cgtcggtcca tgtcgtcaac gtcgacaagc tgacctacgc ggggaatctc gagtcgctga
     1081 caagcgtttc ggggtcccca cgatatgcgt tcgttcgagg ggacatctgc gatctatcca
     1141 tgctcagccg ggttttcgcg gagcaccgcc ccgacgccgt catgaacctc gcggcggaga
     1201 gtcacgtcga ccgctcgatc gatagctccg acatgtttct tcagaccaac ctcgtcggca
     1261 ccgggcgtct gctccaagca gcgcgcgcgt actggagcgg gctctccgcc gagcagcggc
     1321 aggcgtttcg gtttctgcac gtatcgacgg acgaggtgtt cggcgacttg ggcgaaggcg
     1381 gcgctccgtg tcgcgagacg gcggcctacg cgccgagctc gccctacgcg gcgacgaaag
     1441 ccggctccga tcatctggtc agggcgtggt cgcgaaccta cgagctgccc gtgttattga
     1501 cgaactgctc gaacaactac gggccgtacc agtttcccga aaagctgatt cctcacacga
     1561 ttctgcgggc gctgcgcggc gcatcgttgc cggtctatgg tgacggccga cacgtcaggg
     1621 actggcttta cgtcgacgat catgcgcgcg cgctttacac ggtgctcacg aagggccggt
     1681 ccggcgagac gtacaacatc ggcggcaacg gcgagcagcg caacatcgaa gtcgtgagac
     1741 aactctgcca tatcctgaat gccgctctgc cgtcgcagcg gccgccgcgc ggcttcgagt
     1801 cgctgatcgc gtttgtaacc gaccggccgg gccatgatcg tcgttatgcc atcgacgcgc
     1861 acaagatcga gaaggagctg cactggcggc cggccgaaac tttcgcgagc ggactggaaa
     1921 agacagttca gtggtacctc gaaaatcgcg gctggtggca acgggtgctc gatggcagct
     1981 acggtctcga gcgcgccggc ctcaaggtga ccgaatgacg cgccgcggca tcgtgctcgc
     2041 cggcggagcc gggacacgtc ttcatcccgt tacgcttgcc gtatgcaagc agctattgcc
     2101 catctacgac aagccgatga tctattaccc gctgtcgacg ttgatgctgg cgggcatccg
     2161 aacggtgctc atcatctcga cgccgcaaga cacggcgctt ttcgagcagc tcttgggcga
     2221 cggcgcacgg ctcggtatgg acatttccta tgccgtgcag ccgcatcccg gcggtctcgc
     2281 gcaggcgttc ctcatcggcg aacggttcgt gcgcgccgag ccgagcgcgc tgatcttagg
     2341 cgataatcta tttttcggca gcggcctaca ggggttggtc cgcgaggctg ccgcggacat
     2401 cgagggcgcg accgtgttcg catatcgcgt cgcgactccc gaggcattcg gggtagtcgg
     2461 gttcgacgag gcgggccgcg taacctcgat cgaagaaaag cccgcgatac caaagagcaa
     2521 ctacgccgtg acggggctgt acttctacga tgcgcgcgtc gtagagatcg ccaagtcgtt
     2581 gaagccatcg gcgcgcggcg agctcgagat cactgacatc aatcgtgcct acctagaccg
     2641 gggcacgttg aaggtgcgcc gcatggcgcg cggcatggcg tggctcgaca ccggcactca
     2701 cgactcgctg ctcgaggcgg ctcacttcat gcaaacgctc gagaagcggc agggcctcaa
     2761 ggtcgggtgt cccgaagaag tggcttggcg cttgggttgg atcgacgacg gccagctgat
     2821 gaagctcgcc gagccttttg cagatcgtgc gtacggtcgc tatctacttg ccctcttgga
     2881 gcaggcttga tagtcgcgca catcgagctc tgcaaagcgc cggcagagca tcgtcaggcc
     2941 gggctggcgc gccgccgatg aacggcttag tgctcggcga ttcgggccag ctcgctaccc
     3001 acctcaaaga tctgcttcgc gatgccgaat actggggcag gcggaggttc gacttgtcga
     3061 agccagccgg tctcgccgac gaaatactcg ccatgcggcc ctccttcatc gtgaac
//
LOCUS       DATLEE010000215         5304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6081396, whole genome shotgun sequence.
ACCESSION   DATLEE010000215 DATLEE010000000
VERSION     DATLEE010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6081396"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..598
                     /locus_tag="VM692_04600"
     CDS             92..598
                     /locus_tag="VM692_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005492912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUQ51478.1"
                     /translation="MTSTNSIADDSVVSFHYTLRDDAGSITESSDGGSPVVYLHGRNN
                     IVPGLETQLAGKASGDKFTAIVTPEQGYGVRNENAIQRVPLKHIATRGPFQPGQMVVV
                     NTREGGRQARVIKVGHFNVDLDLNHPLAGKTLTFEIEIVDVRAATEEELAHGHAHGPE
                     GHGHDHDH"
     gene            618..1775
                     /locus_tag="VM692_04605"
     CDS             618..1775
                     /locus_tag="VM692_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HUQ51479.1"
                     /translation="MSLALEHVVVLFGALQGVFIAIALCAKTTEPRAPNRLLSALLLC
                     CSALLVEYWVVDSQTFRSVPHLLLCSAPAVFALGPLYYQYTRTLLRAAYRRSDLYHFA
                     PAAAVAVALLPFYLRGGATKVEWTDGWLEEGFVTLPVLPYLLVALASFHLLAYFLKAY
                     EFLESFRRGVVEEFASPRLGTVLWLKRLSLAFSVLMVLFYIGWGEALYPQDRERIVVP
                     VVAMTLALLILGVGLALFLAPELFATYTHGNLPSPDALAEQPKYEKTALTEAQLGSYL
                     ERLEAYVAEHKPYLEGELRLSEVAAALDVPAHHLSQTINQALGVSYFELMNGYRVEAA
                     KALLAESKTSGGTILEIALRAGFGSKASFNRIFKRHTGLTPSQFQAETPFP"
     gene            1888..2319
                     /locus_tag="VM692_04610"
     CDS             1888..2319
                     /locus_tag="VM692_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51480.1"
                     /translation="MSSLRSVKWPLVLVAALGTQVFLLLADFIFVTYYAQLSVTGRDT
                     TYYMQFARETAGAFVFCFAPASIYLIASWLCRRGAEAPYLHAASYIAGYYVLDYAVIA
                     SVARDEIGETLRLPFLLNALAMLVSALVAAWVVARRRPVPA"
     gene            complement(2316..2738)
                     /locus_tag="VM692_04615"
     CDS             complement(2316..2738)
                     /locus_tag="VM692_04615"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51481.1"
                     /translation="MKRLLLGLTAGVALALPLASSAHHSAAVLYKLDQQITVEGTVTR
                     FTLGNPHARIFLTVKSANGTSAEWMAEGGSRTVMLRNGWTGDEVKAGDAIKIIGNPSR
                     DGTNVVHWQRLVLPTGAELWGEDVPEDSALEQLRQRRR"
     gene            complement(2735..3577)
                     /locus_tag="VM692_04620"
     CDS             complement(2735..3577)
                     /locus_tag="VM692_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51482.1"
                     /translation="MSSKLPLSLAFLAALATAGCGTNDASAPSAAARTSPDFTGVWSG
                     AFTTQKNEFWELEDLTACFPGCTPTARAYYASIIEDPANDAKPVQELWGMATAFMREE
                     LAKKSTPDGVAIQTANTEANDPNFRCEPYGLARAAVNPLPIEISRDGANLKIRYEEWN
                     QTRTIYMDGSRHPKNLMPTRLGHSVGRYDGDALVIETKGVEPNLYYDFQSGGGHSAEA
                     TFVERYVIEEDPRRLELDLTITDPVTLLQPHVIHKTWLATPEIQLVEDRCGDVPGKFP
                     EGAL"
     gene            complement(3692..>5304)
                     /locus_tag="VM692_04625"
     CDS             complement(3692..>5304)
                     /locus_tag="VM692_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ51483.1"
                     /translation="PFASPVPNNPTFNPTAGGTVPTFFDNNNNGTFQAGIDVPYDSRF
                     VTAGTYDSYALYINDGLSTPSPLFQGGTTGANTDLFKPYVEEPINRFDSWDTSVNVNW
                     QITDNLSLLYVTSYRTYDNFFAEDTDGSPLAVQQLLQHLDHEQRTQELRFGVTAGKAD
                     LTFGYFYLDQDTAEDARVDLPYVGFDFIHGPDLVPAKNEALYAHASLDLTERLNLSLG
                     LRHSEDEKSYTFRRRNPDLTPVQACTTFWFWEAPNPPNCGVFGLDLASVSYSSDSTDW
                     RVALSADLGETSMVYGQISTGYKAGGNNARPFFPSQLNAFKPETLDSTEIGFKTTLGG
                     KARMNAAVFWNDYADIQLPTTICAWAPPGQQTPCASQNNVGDADVWGIEFEVEWHPID
                     GLTLDASLSTLDFEYTRIQPGATAVTLGMITPYTPETKASVGLQYGFSLPAGGTLTPR
                     LDVAYQDDIYANAVNADTNFIPAVTLSNARLTWNSSGEEWQAALEVTNLADKYYYVTL
                     FDLWGPAGYIHGQPSRPREWALTLKRSF"
BASE COUNT          887 a         1710 c         1688 g         1019 t
ORIGIN      
        1 attccggcgg cgacgacgcg ccgaaacgcc ggccgccgcc cggattaaag gcgaaaagaa
       61 agcatcatcg atcacaaacc gaagagcgct catgactagc acgaactcga tcgccgacga
      121 cagcgtcgtc tcgttccact acacgctccg cgacgatgct gggtcaatca ccgagtcgtc
      181 cgacggcggc agccccgtcg tttatctgca tggccgcaac aacatcgtgc cgggtctcga
      241 gacgcagctc gcgggtaagg cgagcggcga caagttcacc gcgatcgtga cgccggagca
      301 gggctatggt gtgcgcaacg agaacgcgat ccagcgcgtg ccgctgaagc acatcgcgac
      361 acgcggcccg ttccagcctg gacagatggt cgtcgtgaac acgcgcgagg gcggcaggca
      421 agcgcgcgtc atcaaagtcg gccacttcaa cgtcgatctc gacctcaatc atccgctcgc
      481 cggcaagacg ctgacgttcg agatcgagat cgtcgacgtg cgtgccgcga ccgaagaaga
      541 gctcgcacac ggtcatgcgc acgggcccga aggtcatggc cacgaccacg accattagcg
      601 cgggttgacg gccggctatg agcctcgcgc tcgagcacgt cgtcgtcttg ttcggtgctt
      661 tgcagggcgt cttcatcgcg atcgcgcttt gtgccaaaac gacggagccc cgcgccccga
      721 atcgcctcct gagcgcgctg ttgctgtgtt gctcggcgct gctcgtcgag tactgggtcg
      781 tcgactcgca aacgttccgc agcgtgccgc acttgctgct ctgcagcgcg cccgccgtat
      841 tcgcgctcgg cccgctgtat taccaataca cgcggacgtt gctgcgggcc gcgtatcgcc
      901 gcagcgatct ctatcatttc gctccggccg cggcagtcgc cgtcgcgctg ctgccttttt
      961 acttgcgcgg cggcgcgacc aaggtcgaat ggaccgacgg ttggctcgag gaaggcttcg
     1021 tcacactgcc ggtgctgcca tacctgctcg ttgcgctggc gagcttccac ctgctcgctt
     1081 atttcctgaa agcctacgaa ttcctcgagt cgttccgccg cggtgtcgtc gaggagttcg
     1141 caagcccgcg gcttggcacg gtgctctggc tgaaacgcct gtcgctcgcg ttcagcgtcc
     1201 tcatggtgct gttctacatc ggctggggcg aagcgctgta cccgcaggat cgcgagcgaa
     1261 tcgtggtgcc cgtcgtcgcg atgacattgg cgctgctgat tcttggggtc ggtctcgcgc
     1321 tgtttctcgc ccccgagctg ttcgcgactt acacgcacgg caacttgccg agccccgatg
     1381 cgctggccga gcagccgaaa tatgagaaga cggccctgac cgaggcgcag ctcggctcgt
     1441 atctcgagcg tctcgaagcc tatgtcgccg agcacaagcc ctatctcgag ggcgagctcc
     1501 ggctgtcgga ggtcgcggcg gcgctcgacg tgccggcaca ccatttgtcg cagacgatca
     1561 atcaggcgct cggtgtcagt tatttcgagc tcatgaacgg ctatcgcgtc gaagccgcca
     1621 aagcgctttt ggccgagtcc aagacgtccg gcggcacgat actcgagatt gcgctgcgcg
     1681 ccggcttcgg cagcaaggcg tcgttcaacc gcatcttcaa gcgccataca gggctgacgc
     1741 cgtcgcagtt ccaagccgaa acgccttttc cgtaagcgca aggattaacc gttaagcgag
     1801 tgccaccttg caaggtgagg cgcaagttgg gggcgacacg cctacgttgt cgccgtcaac
     1861 aaccaaccgc tcccccggag aacgctcatg tcgtcgctgc gatccgtcaa atggcctctc
     1921 gtgctcgtcg ccgccctcgg cacgcaggtc ttcttgctct tggccgactt catcttcgtc
     1981 acgtattacg cgcagctgtc cgtgaccgga cgcgatacca cttactacat gcagttcgcg
     2041 cgcgagacgg cgggcgcgtt cgtgttctgc ttcgcgcctg cgtccatcta tctgatcgcg
     2101 agctggctgt gccggcgagg cgccgaggcg ccgtacctcc acgccgcgtc gtatatcgcg
     2161 ggctactacg tgctcgacta cgccgtgatc gcgagcgttg cgcgcgacga gatcggcgag
     2221 acgctgcggc tgccgttcct gctgaacgct ctggcgatgc tcgtcagcgc gctcgttgcg
     2281 gcctgggtcg tcgcgcggcg acggcctgtg ccggcctaac gccgccgctg gcgcagctgt
     2341 tcgagcgccg aatcctcggg gacgtcttcg ccccagagct cggcgcccgt cggtagcacg
     2401 aggcgttgcc agtgcacgac gttcgtgccg tcgcgcgacg ggttgccgat gatcttgatc
     2461 gcgtcgccgg ctttcacttc gtcgccggtc cagccgttgc gcagcatgac ggtgcgcgag
     2521 ccgccttccg ccatccattc ggcggacgtg ccgttggcgg acttcacggt caggaagatc
     2581 cgcgcgtgcg gattgccgag cgtgaagcgc gtgaccgtgc cttcgaccgt gatctgctga
     2641 tcgagcttgt agagcaccgc ggcggaatgg tgcgcactcg aggccagagg cagcgccagc
     2701 gccactccgg ccgtcaaacc gagcagcagt cgcttcatag cgccccctcc ggaaatttgc
     2761 cgggcacgtc gccgcagcga tcttcgacga gctgaatctc gggcgtcgcg agccacgtct
     2821 tgtgaatgac gtgcggctgg agcagcgtca cggggtcggt gatcgtcagg tcgagctcca
     2881 ggcggcgcgg atcctcttcg atgacgtagc gctcgacgaa agtcgcctcg gcgctgtgcc
     2941 cgccgccgct ctgaaagtcg tagtagagat tcggctcgac gcccttcgtc tcgatgacga
     3001 gcgcatcgcc gtcgtagcgg ccgacggagt ggccgagccg cgtcggcatc aggttcttgg
     3061 gatgacggct accgtccatg tagatcgtgc gcgtttgatt ccactcctcg taccggatct
     3121 tgaggttggc gccgtcgcgg ctgatctcga tcggcagcgg attcacggcg gcacgcgcga
     3181 gcccgtacgg ctcgcagcga aaattcggat cgttggcttc cgtattcgcc gtctggatcg
     3241 cgacgccgtc gggcgtcgac tttttcgcga gctcctcgcg catgaacgcc gtcgccattc
     3301 cccagagctc ttgcacgggt ttcgcgtcgt tggccgggtc ttcgatgatg cttgcgtagt
     3361 aggcgcgtgc cgtcggcgtg cagccgggga agcacgccgt caggtcctcg agctcccaga
     3421 attcattttt ctgcgtcgtg aaagcgccgc tccacacgcc cgtgaagtcc ggcgacgtgc
     3481 gcgcagcggc gcttggtgcg ctcgcgtcat tcgtgccaca gcccgcggtc gcaagcgccg
     3541 caaggaacgc aagactcagc gggagctttg agctcatacg acgtcaccgt ggcatcggcc
     3601 aacgcagtat aaccggtgcc tctcaggagg cgcccccaag aaaagacggc caggggcaag
     3661 ccctggccgt gcattgtcga gaccgacgcg cttagaagct gcgtttcagc gtcagcgccc
     3721 actcgcgcgg ccgcgacggc tgcccgtgga tatagcccgc ggggccccac agatcgaaca
     3781 gcgtcacgta gtagtacttg tccgcgagat tcgtgacttc caaagccgct tgccactctt
     3841 cgcccgacga attccaggtc aaacgcgcgt tgctcagagt gacggccgga atgaagttcg
     3901 tgtcagcgtt cacggcgttc gcatagatgt cgtcctgata ggcgacgtcg agccgcggcg
     3961 tcagcgtgcc gccggccggc agcgagaagc cgtactgcaa gccgacgctg gctttggtct
     4021 ccggcgtgta cggcgtgatc atgccgagcg tcacggccgt ggcccctggt tgaatacgcg
     4081 tgtactcgaa gtcgagcgtg ctcaacgacg catcgagcgt cagcccatcg atcggatgcc
     4141 actcgacttc gaactcgatg ccccaaacat ccgcgtcgcc gacgttgttc tggctcgcgc
     4201 acggagtctg ctgacccggc ggcgcccagg cgcagatcgt cgtcggcaat tggatgtcgg
     4261 cgtagtcgtt ccagaacacc gcggcgttca tgcgggcttt gccgccgagc gtggtcttga
     4321 agccgatctc cgtgctgtcc agcgtctcgg gcttgaacgc gttcagctgg ctcggaaaga
     4381 acggccgcgc gttgttgccg ccggctttgt agcctgtcga gatttggccg tagaccatcg
     4441 aggtctcgcc gaggtcggcc gagagcgcca cgcgccaatc cgtgctgtcg ctcgagtagg
     4501 agaccgacgc cagatccaag ccgaacactc cgcagttcgg cggattcggc gcctcccaga
     4561 accagaacgt cgtgcacgct tgcaccggcg tcagatccgg attgcggcgg cggaacgtat
     4621 agctcttctc gtcctcggaa tggcgaaggc cgagcgagag attgaggcgc tccgtcaaat
     4681 ccaacgacgc atgggcgtac agcgcctcgt tcttcgcggg caccaaatcc gggccgtgga
     4741 tgaaatcgaa cccgacgtac ggcaaatcga cgcgcgcatc ctcggccgta tcttgatcga
     4801 ggtagaaata gccgaacgtc aggtccgctt tgccggccgt cacgccgaag cgcagctctt
     4861 gcgtgcgctg ctcgtgatcc aaatgttgca gcaactgctg caccgcgagc ggcgagccgt
     4921 ccgtgtcttc ggcgaagaag ttgtcgtacg tgcggtacga cgtcacgtac agcaaggaaa
     4981 ggttgtccgt gatctgccaa ttcacattga cggacgtgtc ccacgaatcg aatctgttga
     5041 taggctcctc gacgtacggc ttgaacagat ccgtgtttgc gccggtcgtg ccgccttgga
     5101 acagcgggct tggcgtgctc aagccgtcgt tgatgtagag cgcgtacgag tcgtacgtgc
     5161 cggcggttac gaaccggctg tcgtacggaa cgtcaatgcc cgcttggaaa gtgccgttgt
     5221 tgttgttgtc gaagaacgtc ggcaccgtgc cgcccgcggt cgggttgaac gtcgggttgt
     5281 tcggcaccgg tgaagcgaac ggcg
//
LOCUS       DATLEE010000216         2239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4704560, whole genome shotgun sequence.
ACCESSION   DATLEE010000216 DATLEE010000000
VERSION     DATLEE010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4704560"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="VM692_04630"
     CDS             complement(<1..1665)
                     /locus_tag="VM692_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUQ51484.1"
                     /translation="MVKRVDKSSTTSVDRRKFLQGAAAGAAGAAGAIVAGTGDAQQTT
                     PAPRPAVTRPTEAAGRADFVPPPEPRADARFIQHPASDYMVDVFKALDFDYVAVNPGS
                     AFEGIHESLINHGGNKKPEILTVLHEEAGAAIAHGYAKAAGKPMMTLMHGTVGLLHAS
                     MGLFQAWCDRVPVFAVVGHPRNPTSVVNRPHSAQDMGAIVRDFMKMDDEPTNIEVFGN
                     VAMRAYTMGRTPPMGPTLLVVDSELQEAAIEDPSKLTVPRLVMPSFPQGDAGAVREAA
                     RLLATAERPLIRTGKLARTPAGWDRLIELAELLQAPVDVGGYASWQDFPSWHALYGTG
                     GPDYRPDVILGLELNDMTASVRQVRAAGGKTISICSEYLSQGHNIHDYGNYSEVDVPI
                     AADGEVTLPALIEEIRRQSASSAVRARAARGDQIATAHKTIREREIDDARYGWNSSPI
                     SVPRMVAELGRQIQNDDWAIVSGHQFTGQWQRRLLNHDKVYRYNGDCGGFGIGYDTPA
                     SVGGALAHREHGRLPIGIVGDGDFNFVGAGALWTAAHHKIPLLLIVH"
     gene            complement(1808..>2239)
                     /locus_tag="VM692_04635"
     CDS             complement(1808..>2239)
                     /locus_tag="VM692_04635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HUQ51485.1"
                     /translation="AFATADQRNTPVALALLDLDNFKRVNDNHGHQTGDEVLRRVASR
                     LLSAVRGDDAICRLGGDEFLVLMADTDATIANRVAERIRHAITDTPVPTRDGAMAVSV
                     SVGCTIRLPRDTTAVEALLERADQALMQSKAAGRNRVRMAN"
BASE COUNT          344 a          725 c          782 g          388 t
ORIGIN      
        1 gtgcacgatc agcagcagcg gaatcttgtg atgcgcagcc gtccagagcg cgccggcgcc
       61 cacgaagttg aagtcgccgt ccccgacgat gccgatcggc aaccggccgt gctcgcgatg
      121 cgccaacgcg ccgccgacgg aggccggcgt gtcgtagccg atgccgaagc cgccgcaatc
      181 gccgttgtag cgatagacct tgtcgtggtt caacagccgg cgttgccact ggcccgtgaa
      241 ctggtggccc gagacgatgg cccagtcgtc gttctgaatt tgtcgcccga gctcggcgac
      301 catgcgcggc acgctgatgg ggctcgagtt ccagccgtag cgagcgtcgt cgatctcgcg
      361 ctctcggatc gttttatgcg cagtcgcaat ttggtcgccg cgcgcggcgc gtgcacgcac
      421 agcgctgctc gcactctgcc ggcgaatctc ctcgataagg gccggcagcg tcacctcgcc
      481 gtcggccgcg atcggcacgt cgacctcgga gtagttgccg tagtcgtgga tgttgtgacc
      541 ctgcgagagg tactcggagc agatgctgat cgtcttgccg cccgcggcgc gcacttggcg
      601 cacggacgcc gtcatgtcgt tgagctcgag gccgaggatc acgtccgggc gatagtcggg
      661 gccgcccgtg ccatacagcg cgtgccacga cgggaagtct tgccacgagg cgtaaccgcc
      721 gacgtcgacg ggcgcctgca acagctcggc gagctcgatg aggcggtccc aacctgccgg
      781 cgtgcgcgcg agcttgccgg tgcgaatcaa cggccgctcg gccgtcgcga gcaggcgagc
      841 ggcttcacgc acggcgcccg cgtcgccctg cggaaagctc ggcatgacca agcgcggtac
      901 ggtgagcttg ctgggatcct caatcgcagc ctcttgcagc tcggagtcga cgacgagcag
      961 cgtagggccc atcggcggcg tgcggcccat cgtgtaggcg cgcattgcga cgttgccgaa
     1021 cacttcgatg ttcgtcggct cgtcgtccat cttcatgaag tcgcgcacga tcgcgcccat
     1081 gtcttgcgcg ctgtgcgggc gattgacgac gcttgtcggg ttgcgtggat gaccgacgac
     1141 ggcaaacacg ggcacgcggt cgcaccaggc ctggaacagg cccatggacg cgtgcagcag
     1201 cccgacggtg ccgtgcatca gcgtcatcat cggcttgccg gcggctttcg cgtagccgtg
     1261 cgcaatcgcg gcgccggctt cctcgtgcaa taccgtgaga atctcgggct ttttgttgcc
     1321 cccgtggttg atcaacgatt cgtgaatgcc ttcgaacgcc gacccgggat tgacggcgac
     1381 gtagtcgaag tcgagcgcct tgaagacgtc taccatgtaa tccgaggccg gatgctggat
     1441 gaaccgcgcg tcggcgcgcg gttcgggagg cggaacgaag tcggcgcggc ctgccgcttc
     1501 cgtgggccga gtgacggcgg ggcggggcgc aggagtcgtc tgttgcgcgt cgccggttcc
     1561 ggcgacgatg gcaccggccg ctccggcagc gcctgcggcc gctccttgca ggaacttgcg
     1621 acgatcgact gacgtcgtcg aggacttatc gactcgtttg accacggtat cccccttcgg
     1681 ggactgccaa gggcgtcccc cgttgattcg cggggctgcc gaagaggcag atcccgacgt
     1741 cgagggatga taccgcaacg aaaaagacga ggaaggtcgg ggcggtttgg cggacgcgcc
     1801 gcgccggtca gttcgccatg cggacgcgat tgcgtcctgc ggccttcgat tgcatcagcg
     1861 cctggtcggc gcgttcgagc agagcctcga cggccgtcgt gtcgcgcggc agccgaatag
     1921 tgcacccaac gctgacgctg accgccatcg cgccgtcccg cgtcggcacc ggcgtatccg
     1981 tgatcgcgtg gcggatgcgc tcagcgacgc ggttggcgat cgtggcgtcg gtgtccgcca
     2041 tcagcacgag aaactcgtcg ccgccgagcc ggcagatcgc atcgtcaccg cgcacggcgc
     2101 tcaataggcg gctggccacg cgacgcagca cctcgtcgcc ggtctggtgc ccgtggttgt
     2161 cgttgacgcg tttgaagttg tcgaggtcga gcaaggccag ggcgaccggc gtgttgcgtt
     2221 gatcggccgt ggcgaaagc
//
LOCUS       DATLEE010000217         5850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7573242, whole genome shotgun sequence.
ACCESSION   DATLEE010000217 DATLEE010000000
VERSION     DATLEE010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7573242"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VM692_04640"
     CDS             complement(<1..188)
                     /locus_tag="VM692_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="with TatABE forms the twin-arginine translocation
                     complex which is involved in the transport of proteins
                     across the cytoplasmic membrane; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HUQ51486.1"
                     /translation="MSSLSLDPNVERVGRSLLAAALELRSRGLKAILCLLGLFAGLFP
                     FANDIFRIVAEPIMAAMPA"
     gene            292..1143
                     /locus_tag="VM692_04645"
     CDS             292..1143
                     /locus_tag="VM692_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ51487.1"
                     /translation="MHSRSRLVTAALVLGGLTFAAQAQFGTEPAKLELIKLQDDLYVI
                     HNEAVPGNTTVLVTNEGVILVDDKFELDVDNVVKMVKTVTNQPIKYVINTHFHGDHSG
                     GNAKLQAAGTLAVASAAALTRMVAAKQSGQPDITIEPRGSIHLGGKSAEIYWFGRAHT
                     DGDVVVLFPQNRTLAAGDMFTVGEGTPQLIDYAGGGSAKEWTATVTKALALDFDTVVP
                     GHGNVVKKADMQAFRASTARLTELVTQLVRQNKSRADIEAAMRSEFGWQDFHVQMALD
                     GLINEMR"
     gene            1250..2002
                     /locus_tag="VM692_04650"
     CDS             1250..2002
                     /locus_tag="VM692_04650"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HUQ51488.1"
                     /translation="MKRPSLLVAAVLALVVAGAAYGQFFGGGRGRRGYSIDDSHIPPP
                     TEFVSMRWHFGTNGAIGHMGWSHNYPDSEIHFNEFIGRTTKIDVADDSYRLLELSSPE
                     IFEYPYAYISEPGEMELTEAEVLNLREYIDRGGFILIDDFDTWHLDNLKEEMLRVFPD
                     RVFERMAVDNPIFDLAYAVESLTSMAPYVAGGEPAYYSLKNSQGEIAIIALHNNDFAN
                     FWDWYGSPQYPLRPSTDAFRMGTNFVVHSMTH"
     gene            2016..2951
                     /locus_tag="VM692_04655"
     CDS             2016..2951
                     /locus_tag="VM692_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUQ51489.1"
                     /translation="MTEHTETTRREFIVRSSAAAAACGLAGCAGLDTVARSGVPQRPI
                     PSTGEMLPVIGLGSSKVVEQIAQNGTEPLANVLRALVAHGGKLVDTWPRNAANDAGLG
                     RVLALPELRDKLFVTTKVDQVGKDVGVRQFRDALRNYQRETIDLVQIFSLTDLDTHWP
                     SLKEWKAQGAARYIGVTVSQDSRHADLEQFLRRERPDFVQMNYSITERAVEERLLPFA
                     ADRGAAVVINRPFMNGAYFDRLEGKPLPEWAREVGCATWAQFSLKYILANPSLTSVLT
                     ETSDPKHMEENLLAAVGPLPDAAARQRMREFIATV"
     gene            3018..3602
                     /locus_tag="VM692_04660"
     CDS             3018..3602
                     /locus_tag="VM692_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HUQ51490.1"
                     /translation="MWRAFVLIVLLPQLAGCGYNAFQTQDEQIKASWAEVVNQYQRRA
                     DLIPNLVETVRGFAQQERDVLIGVTEARSKVGSVQATPEVIDDPQKFAQFQAAQGELS
                     SALSRLMVVVERYPELKSDQNFRDLQAQLEGTENRITVARNRYIEAVQTYNGTVRRFP
                     NNLTAKVFGYDVKPTFTVENEAEISRPPQVSFGR"
     gene            3613..4491
                     /locus_tag="VM692_04665"
     CDS             3613..4491
                     /locus_tag="VM692_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HUQ51491.1"
                     /translation="MSCFSVAARFMALALGAWLLAGGEPAFAQQAVPPLTGHVVDTTG
                     TLATEAVASLDAKLAAFEQRKGSQIAALLVRTTAPEALEQYSLRVAEQWRIGRGGVDD
                     GIVLVAALDDRRMRFEVGYGLEGAVPDALARRIIAETIAPRFYEEDYAGGLDAGLDAL
                     IGLIDGEPLPVPVAREPQAEPFTALPIVLVLALVAAPVFRRMFGSLLGSVALGAGAGF
                     VVWLISSVLFASLVAGAMVFVLALTGMGGGGGRWASRGGWGGMPGGFGRGGGGFGGGF
                     RGGGGRFGGGGASGGW"
     gene            4494..4982
                     /locus_tag="VM692_04670"
     CDS             4494..4982
                     /locus_tag="VM692_04670"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HUQ51492.1"
                     /translation="MRVLSHLILPRSALARRFPSHALTTIEEAVEASEKLHRAEIRVA
                     IEVALDVRALWRVRSARERAHEVFTELAVWNTRERSGVLIYVLLAERDVEIVADSGFD
                     GRVGESEWQRVCTIIEREFAAGRWRDGVVRGIEAVTGLLTHAFPAVGRNPDEQQNRPA
                     VL"
     gene            5069..>5850
                     /locus_tag="VM692_04675"
     CDS             5069..>5850
                     /locus_tag="VM692_04675"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUQ51493.1"
                     /translation="MSIRKFNSVLLGAWLAVVPALASHAETATPQSVGLSADRLQRVH
                     ELVERTMAAGEISGAVTLVARNGQVAYLEAQGVMDLASKKPMQPDSMFRMASMSKPVA
                     AVAILMLAEEGKVRLNDPVSRFIPAYANLEVGIAKPVPPGPAPAAQAGPGGAPPAFYK
                     APAERQITVLDLLTHTSGFMSGPMGNSVANAAFNKRHELGLKWVEELGPSPLEFQPGT
                     RWAYSAVAGFDALAHIVEVAAGQEYSAFLRERLFGPLGMRDVT"
BASE COUNT          991 a         1906 c         1998 g          955 t
ORIGIN      
        1 gccggcattg cggccatgat cggctcggcg acgatgcgaa agatgtcgtt cgcgaacggg
       61 aataggccgg cgaacaaacc gagcaggcac aagatcgctt tgagcccgcg cgagcgcagc
      121 tcgagcgcgg ccgcgagcag gctgcggccg acgcgctcga cattcggatc gagggatagg
      181 ctggacacgc gcctattcta atcgacgcgg ggcgatcaag ccgtggaggc ctgtagtacc
      241 atgcgggctc tggattcgaa gctcacgccc tcttgccgga gatcgcacgt catgcacagc
      301 agatcgagac tcgtcaccgc cgcgctcgtt ctcggcggcc tcacgttcgc ggcgcaggcg
      361 cagttcggca cggagccggc caagctcgag ctcatcaagc tgcaagacga tctctacgtc
      421 atccacaatg aagcggtgcc cggcaacacg acggtgctcg tcacgaacga gggcgtcatc
      481 ctcgtcgacg ataagttcga gctcgacgtc gacaacgtcg tcaagatggt gaaaaccgtc
      541 acgaatcagc cgatcaagta cgtcatcaac acgcacttcc acggcgacca cagcggcggc
      601 aacgccaagc tgcaggccgc cggcacgctc gcggtcgcgt ctgcggcggc gctgacgcgc
      661 atggtcgcgg cgaaacagtc gggccagccc gacatcacga tcgagccgcg cggctcgatt
      721 cacttgggcg gcaagtccgc cgagatctat tggttcggcc gcgctcacac ggacggcgac
      781 gtcgtcgtcc tgttcccgca gaaccgcacg ctcgcggccg gcgacatgtt cacggtcggc
      841 gagggcacgc cgcagctgat cgactacgcg ggcggcggca gcgccaagga atggacggcc
      901 acggtcacga aggcgctcgc gctcgacttc gacacggtcg tgccggggca cggcaacgtg
      961 gtgaagaagg ccgacatgca agcgttccgc gcgagcacgg ctcggctaac cgagctcgtc
     1021 acgcagctcg tgcgccagaa caagtcgcgg gccgacatcg aagctgcgat gcgctcggag
     1081 ttcggctggc aggacttcca cgtccagatg gcgctcgacg ggctcatcaa cgagatgcgc
     1141 tgagcgtgcg ccgctagcga atctcaagaa atgccgcgtg ctgtgcgcgg cttctgagcg
     1201 agcacggttt cgcggtatcg ttgcaagcga tccgtgaggt gaccgcgcag tgaaacgacc
     1261 gtcgttactc gttgcggcag tgttggcgct cgtcgtcgcc ggcgccgcgt acggccaatt
     1321 cttcggcggc ggccgcgggc gtcgcggcta ctcgatcgac gacagccaca tcccgccgcc
     1381 gacggagttc gtcagtatgc gctggcactt cggcacgaac ggcgcgatcg gtcacatggg
     1441 ctggtcgcac aactacccgg actcggagat ccacttcaac gagttcatcg gccgcacgac
     1501 gaagatcgac gttgccgacg actcgtatcg gctgctcgag ctctcgagcc ctgagatctt
     1561 cgagtatccg tacgcttaca tctccgagcc cggcgagatg gagctcaccg aagcggaagt
     1621 cctgaacctg cgcgagtaca tcgaccgcgg cggcttcatc ctgatcgacg atttcgatac
     1681 gtggcacctc gacaatctaa aagaggagat gctgcgcgtg tttccggacc gcgtgttcga
     1741 gcgcatggcc gtcgataatc cgatcttcga tctcgcctac gccgtcgaaa gcctcaccag
     1801 catggcgccc tatgtggcgg gcggcgagcc ggcctactac agcctcaaga actcgcaagg
     1861 cgagattgcg atcatcgcgc tgcacaacaa cgatttcgcg aacttctggg attggtacgg
     1921 tagcccccaa tacccgctgc gtccgtcgac ggacgcgttc cgtatgggca caaatttcgt
     1981 cgtgcactcg atgacgcact gattcggaga atgcgatgac agagcacaca gagacgactc
     2041 gccgcgagtt catcgtgcgc tcgagcgccg cagctgcggc ctgcggcttg gccggctgcg
     2101 cggggctcga caccgtggct cgcagcggcg tcccgcagcg gccgattccc tcgacgggcg
     2161 agatgctgcc ggtgatcggg ctcggctcga gcaaggtcgt cgagcagatc gcacaaaacg
     2221 gcacggagcc gctcgccaac gtgctgcggg cgctcgtcgc gcacggcggt aagctggtcg
     2281 acacctggcc gcgcaacgcc gccaacgatg cgggtttggg acgcgtgctc gcgctgcctg
     2341 agctgcgcga caagttgttc gtgacgacga aggtcgacca ggtcggcaag gacgtcggcg
     2401 tgcgccaatt ccgcgacgcg ctgcgcaact accagcgcga gaccatcgat ctcgtgcaga
     2461 tcttcagcct gaccgatctc gacacgcatt ggcccagcct caaggaatgg aaagcgcaag
     2521 gcgcggcacg ctacatcggc gtcacggtct cgcaggactc gcggcacgcc gacctcgagc
     2581 agttcctgcg ccgcgagagg ccggacttcg tgcagatgaa ctactcgatc acggagcgag
     2641 ccgtcgagga gcgcctgctg ccgttcgcag ccgatcgcgg cgcggccgtc gtgatcaacc
     2701 gcccgttcat gaacggcgct tacttcgatc gcctcgaagg caagccgttg ccggagtggg
     2761 cgcgtgaggt cggctgcgcg acctgggcgc aattctcgct gaaatacatt cttgcgaacc
     2821 cgtcgctgac gagcgtgctg acggagacga gcgatcccaa gcacatggaa gagaacctgc
     2881 tcgcggccgt cgggccgctg cccgacgccg cagcacggca gcgcatgcgc gagttcatcg
     2941 caaccgtctg atcgcgctag aaacgcccca gttggtcccg atcttgcagc taagccctcg
     3001 aagaggaggg ctggaccatg tggcgagcat tcgtactcat cgtgttgctg ccgcagcttg
     3061 cgggctgcgg ctataacgcg tttcaaacgc aagacgagca gatcaaggcc tcgtgggccg
     3121 aggtcgtcaa ccaataccag cgccgcgccg atctgatacc taatctggtc gagaccgtgc
     3181 gtggcttcgc gcagcaagag cgagacgtcc tgatcggcgt aacggaagcg cgctcgaagg
     3241 tcggcagcgt gcaagcgacg cccgaggtca tcgacgaccc gcagaagttc gcgcagttcc
     3301 aagccgcgca gggcgagctc agcagcgcgt tgtcgcggct catggtcgtc gtcgagcgct
     3361 acccggagct gaagtcggac cagaacttcc gcgacctaca ggcccagctc gaagggacgg
     3421 aaaatcgcat cacggtcgcg cgcaatcgct acatcgaagc cgtgcaaacc tacaacggca
     3481 cggtgcgccg tttcccgaac aacttgacgg caaaagtctt cggctacgac gtgaagccca
     3541 cgttcacggt cgaaaacgaa gccgagatct cgcggccgcc gcaggtgtcg ttcggccggt
     3601 gacggagcgt cgatgagctg tttcagcgtt gcggcgcgct tcatggcgct cgcgctcggc
     3661 gcctggctgc tcgccggcgg cgagcctgcc ttcgcgcagc aagccgtccc gccgctgacg
     3721 ggtcacgtcg tcgacacgac gggaactctg gccaccgagg ccgtcgcaag cctcgacgcc
     3781 aagctggccg cattcgagca gcgcaagggc agtcagatcg ccgcgctgct cgtgcgaacg
     3841 acggcgcccg aggcgctcga gcagtactcg ctacgcgtcg cggagcagtg gcgcatcggc
     3901 cgcggcggcg tcgacgacgg catcgttttg gtcgccgcgc tcgacgatcg ccgcatgcgg
     3961 ttcgaggtcg gctacgggct cgaaggcgcg gtgcccgacg cgctcgcgcg ccgcatcatt
     4021 gccgagacga tcgcgccgcg gttctacgaa gaggactatg ccggagggct agacgcgggg
     4081 ctcgatgcgc tgatcgggct catcgacggc gagccgttgc ccgtgcccgt cgcgcgcgag
     4141 ccgcaagccg agccgttcac cgcgttgccg atcgtgctcg tgctggcgct cgtcgccgcg
     4201 ccggtgttcc ggcgcatgtt cggctcgctg ttgggctcgg tcgcgctcgg tgccggtgcc
     4261 ggcttcgtcg tctggctcat cagcagcgtg ctgttcgcct cgctcgtcgc cggtgcaatg
     4321 gtcttcgtgc tcgcgctgac gggcatgggc ggaggcggcg ggcgctgggc gtcgcgcggc
     4381 ggctggggcg gcatgccggg cgggttcggc cgcggcggcg gtggcttcgg cggcggcttc
     4441 cgcggcggcg gcggccggtt cggcggcggc ggtgcgtcgg gaggatggtg agcgtgcgcg
     4501 tcctgtcaca tctgatcctg ccgcgttcgg cgctcgcacg ccggtttccc tcgcatgcat
     4561 tgacgaccat cgaagaagcc gtcgaggcgt cggagaagct gcatcgcgcc gagattcgcg
     4621 tcgcgatcga ggtggcgttg gacgtgcgtg cgctgtggcg cgtccgctcc gcgcgtgagc
     4681 gcgcacacga ggttttcacg gagctcgccg tctggaatac acgcgagagg agcggcgtgc
     4741 tgatctacgt gctgctggcc gagcgcgacg tcgagatcgt cgccgacagc ggcttcgacg
     4801 gccgcgtagg cgagtcggag tggcagcgcg tgtgcacgat catcgaacgc gagtttgcgg
     4861 ccggccgttg gcgcgatggc gtggtgcgcg ggatcgaagc ggtgacgggg ctgttgacgc
     4921 acgctttccc ggccgtgggc cgcaatcccg acgagcagca gaatcggccg gccgttcttt
     4981 gacggttctg gtcgcggcga cgattgcgcc ggtagcggtt cgcgcggcat gctcttcgca
     5041 tcgaacgcgt catagcaggg ggagcgtcat gtcgattcgt aaattcaact cagtactgct
     5101 cggcgcatgg ctggccgtcg tgccggcgct cgcgtcgcac gcggagacgg cgacgccgca
     5161 gagcgtcgga ctctcggccg accggctgca acgcgtgcac gagctcgtcg agcgcacgat
     5221 ggccgcgggc gagatctcgg gcgccgtgac gctcgtggcg cgcaacggcc aggtcgcgta
     5281 tctcgaggcg cagggcgtca tggacctcgc gtcgaagaag ccgatgcagc ccgactcgat
     5341 gttccgcatg gcgtcgatgt cgaagcccgt cgcagccgtt gccatcttga tgctggccga
     5401 ggaaggcaag gtacgcctca acgatcccgt gtcgcgcttc atccccgcgt acgcgaacct
     5461 cgaggtcggc atcgcgaaac ccgtgccgcc cggtccggcg cctgcggcgc aagcgggccc
     5521 cggcggcgcg ccgcccgcgt tctacaaagc gccggcggag cggcagatca ccgtgctcga
     5581 tctgctgacg cacacgtcgg gtttcatgag cggcccgatg ggcaattccg tcgcgaacgc
     5641 cgcattcaac aaacgccacg agctcggctt gaagtgggtc gaggagctgg gtccgtcgcc
     5701 gctcgagttc cagcccggca cgcgctgggc ttacagcgcc gtcgcgggct tcgacgcgct
     5761 ggcacacatc gtcgaggttg ccgcggggca ggagtactcg gcgttcctgc gcgagcggct
     5821 ctttgggccg ctcggcatgc gcgacgtgac
//
LOCUS       DATLEE010000218         2284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4714199, whole genome shotgun sequence.
ACCESSION   DATLEE010000218 DATLEE010000000
VERSION     DATLEE010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4714199"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..599
                     /locus_tag="VM692_04680"
     CDS             63..599
                     /locus_tag="VM692_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51494.1"
                     /translation="MREVDARVWAALCAEIEQPDIIQALADAETRNASDSRDWNADVE
                     SYRAHLERLKQVEATIMRQFRRGLVSEDALAIETAAAMRERKMVEEQLATAERAAAST
                     TSAQERLRNATAMIERLRAALPNATLEQRQALLRELTFDGGARISLDGRVHLDLRLMR
                     PSTATRTQPDVRVVRSAG"
     gene            complement(592..1173)
                     /gene="recA"
                     /locus_tag="VM692_04685"
                     /pseudo
     CDS             complement(592..1173)
                     /gene="recA"
                     /locus_tag="VM692_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
     gene            complement(1312..>2284)
                     /locus_tag="VM692_04690"
     CDS             complement(1312..>2284)
                     /locus_tag="VM692_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51495.1"
                     /translation="FACPTGTPSCKPLTQLDGPPAAPEAAKADQFVVVDLDNVNADCW
                     HSVGAAGFGVELAAGQLVRPVPEPQWKADNNKPTTIDGVLDIGSHAGELQSPDPNYLR
                     FRVGYVPSDQMPVFTSAPGPDLQLPSTPMLELLSCTFGAQPPVVAPTDLENRFPALAH
                     AEAGYVQTLSGGAKLANGVAATAVINKGANYNASVSSTAPFAGSMKVGGIDLPFRGVR
                     VGVAASALTELTFTPASDAADYYDVLLHEITGNSKQLLRVYTIPNGMLAAPVTVDLRA
                     ITLTPGREYVFEVRSYTGRQTARTGNFELVQERQSLAVVHSATFVVE"
BASE COUNT          343 a          769 c          801 g          371 t
ORIGIN      
        1 atcccgctgt ctacttctgc gcgggcaagc gcgagaatgg ctgtacgctg aagccgccgc
       61 acgtgcgcga ggtcgacgct cgcgtgtggg ccgcgctgtg cgccgagatc gagcaacccg
      121 acatcatcca ggcgctcgcc gacgccgaga cccgcaacgc gtcggactcg cgcgactgga
      181 acgccgatgt cgagagctac cgcgcgcacc tcgagcggct gaagcaggtc gaggctacga
      241 tcatgcggca gttccgccgt ggcctggttt cggaagacgc gctcgcgatc gagaccgccg
      301 ccgcgatgcg cgagcgcaag atggtcgagg agcaactcgc caccgccgag cgcgcggccg
      361 ccagcacgac gagtgcgcag gagcggctcc gcaacgcgac ggcgatgatc gaacggctgc
      421 gggccgcgct gcccaacgcg acgctggagc aacgccaggc gctcctgcgt gagctgacgt
      481 tcgacggcgg cgctcgcatc tcgctcgacg gtcgcgtgca tctcgacctg cggctgatgc
      541 gtccgtcgac cgcgaccaga acgcaaccag acgtgagagt cgtgcgctcc gctggttgat
      601 gaagacgacg atcgtcttcg ttcgcgagat caccgcggtc aacttgcgca gcgcttgcga
      661 catcaagcgc gcttgcagcc ccatgtgcgc gtcgcccatc tcgccttcga tctcggcgcg
      721 cggcgtcagc gcggcgacgg agtcgacgac gatcagatcg atcgcgcccg tgcgcacgag
      781 cgtgtcgcag atctccaggg cttgctcgcc gcagtccggc tggctcacca acagatcctc
      841 gaggtccacg ccgagccggc gtgcgtactc ggtgtcgagc gcgtgctcgg cgtcgatgaa
      901 ggcgcacacg ccgcccattc gctgcgcctc ggcgatcgcg tgcagcgtca gcgtggtctt
      961 gccggaggac tccggtccgt agatctcgat gatgcgacct cgcgggaggc cgccgcagcc
     1021 gagcgcgacg tcgagggcga ccgagcccgt cgggatcacg tcgaccgatt ggaccagcgt
     1081 gccgtcgagc cgcatgatcg atccggtgcc gaactgtttg tggatggcgc ggatcgcttc
     1141 gccgatgctc ttgttcttct tgtcttgaga catgtgccgg ggcacttcca cggttcgtgc
     1201 cgtcgcaacg cgctgtaatt tcagcgagcg gttgtccgaa ctgtccgcct gtcggacgtg
     1261 tccggccggg attcggacgc gccgacgcgc cgacgtgccg acgggccgac gttattcgac
     1321 gacgaacgtc gcgctgtgca ccacggccag gctctggcgc tcctgcacga gctcgaagtt
     1381 gccggtgcgc gccgtctgcc ggccggtgta cgagcgcacc tcgaacacgt attcgcgtcc
     1441 gggcgtcagc gtgatcgcgc ggagatccac ggtgaccggc gcggcgagca tgccgttggg
     1501 gatcgtgtac acgcgcagca gctgcttgct gttgccggtg atctcgtgga ggaggacgtc
     1561 gtagtagtcg gccgcgtcgg acgccggcgt gaacgtcagc tccgtcagcg cggacgcggc
     1621 gacaccgacc cggacaccgc ggaacggcag gtcgatgccg ccgactttca tcgatcccgc
     1681 gaacggcgcc gtgctgctca ccgacgcgtt gtagttggcg cccttgttga tgacggccgt
     1741 cgcggcgacg ccgttcgcca gcttcgcgcc gccgctcagc gtctgcacgt aaccggcttc
     1801 ggcgtgcgcg agcgccggga atcggttctc gagatccgtc ggtgcgacga ccggcggctg
     1861 cgcaccgaac gtgcacgaca gtagctcgag catcggcgtc gacggcagct gcaggtcggg
     1921 accgggcgcg ctcgtgaaca ccggcatctg gtcgctcggc acgtagccga cgcggaagcg
     1981 cagatagttc ggatcgggag attgcagctc gccggcgtgg ctaccgatgt cgagcacgcc
     2041 gtcgatcgtg gtcggcttgt tgttgtccgc cttccactgt ggctcgggca caggccggac
     2101 gagctgtccg gcggcgagct cgacgccgaa gccggccgcg ccgacggagt gccagcagtc
     2161 ggcgttcacg ttgtcgagat cgacgacgac gaactggtcg gctttcgccg cctcgggcgc
     2221 ggccggcgga ccgtcgagct gcgtcagcgg cttgcagctc ggcgtgcccg tcggacacgc
     2281 gaag
//
LOCUS       DATLEE010000219         3008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5467512, whole genome shotgun sequence.
ACCESSION   DATLEE010000219 DATLEE010000000
VERSION     DATLEE010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5467512"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VM692_04695"
     CDS             complement(<1..366)
                     /locus_tag="VM692_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HUQ51496.1"
                     /translation="MRTDLGFKRIATEEAFATKDLIRAYRELLARKGSTDIGFNSLMG
                     YYLENPSERTRLVSERLQDLGQRRIDDMDATGIDLQILSLTSPGVQIFDAATGTALAR
                     ATNDELAAAIAAHPTRYAGL"
     gene            complement(385..1716)
                     /locus_tag="VM692_04700"
     CDS             complement(385..1716)
                     /locus_tag="VM692_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ51497.1"
                     /translation="MTRAINVSDVIDTSAVGSFQLRVFLLCAAVLFVDGFDVQGITYV
                     VPTLTSEWGLVRGQLWPALTGGLIGVMLGAMFLAPLADRVGRRKVIVYSCLAFGVLTL
                     LTPAVGSLLPLVALRFFTGLGLGAALPNAIGLASEYAPHRRRALIVMFVGSGISLGAI
                     GAGAAAARFVGPYGWESMFVVGGVLPLLLAAALARWLPESIRFAALIPSRQSAANRLL
                     RMIKPEIGADAAIISHDPEGGKATLRALFSDGRGIATVCLWVAFFMSLLNVYLAINWL
                     PTSLEASGFSRTQASVMTTLYHVGGVIGTYAIGLLMDRLGAHRMVLVGLAFAAVGFYT
                     FATATDLTQWPTTAVLMLAGTGVIGAQVGATALASMTYPVAMRATGLGWALGVGRVGS
                     IVGPTILGLMLATADARSVYIWCLAPISIGMLAVALLKRQATPVPQPVTAS"
     gene            1858..2745
                     /locus_tag="VM692_04705"
     CDS             1858..2745
                     /locus_tag="VM692_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HUQ51498.1"
                     /translation="MQDRNPYAAPQTNVTQGDGPVEYGEVKIFSVSGRLGRVRYIGYS
                     VGVSLLIMLGLGIVAAIVGAGDVNRMFLVGLAGYALILVVQLMLTIQRAHDMNTTGWL
                     ALIWFVPLAQLVFWLVPGTRGENDYGKQPPPNTIGVIVLACVVPLVFVGGILAAIAIP
                     AYQDYTIRAQVSEGLSLAAGIKAAVAEAFARTESAPRDRLEAGLPEDATDTSGQYVTS
                     VDIADGTILINYGANAHSTIAGAVLALQPYVMPDNSVVWRCGEAAEPPGAAALDGSAL
                     SSDEATDIGPRYLPSACRP"
BASE COUNT          542 a         1001 c         1006 g          459 t
ORIGIN      
        1 aagtcccgcg tagcgcgtcg ggtgcgccgc aatcgccgcc gccagctcgt cgttcgttgc
       61 gcgtgcgagc gcggttcccg tcgcggcgtc gaagatctgc acgcccggcg acgtcagcga
      121 caagatctgc agatcgatac ccgtcgcgtc catgtcgtcg atgcggcgct ggccgagatc
      181 ctggagccgc tcgctgacca gacgcgtgcg ctcgctgggg ttctccaagt agtagcccat
      241 gaggctgttg aagccgatgt cggtcgagcc cttgcgcgcg agcagctcgc ggtaagcgcg
      301 aatgaggtcc ttggtcgcga acgcttcttc agtggcgatg cgtttgaagc cgagatcggt
      361 acgcaatgga gtgctccagt cgagttagct tgccgtgacc ggttgcggca ccggcgtggc
      421 ctgacgcttc agcaatgcga cggctagcat gccgatcgag atgggtgcca ggcaccagat
      481 gtagaccgag cgcgcgtcgg ccgtcgccaa catgaggccg agtatcgtgg gcccgacgat
      541 cgagccgacg cggccgacgc cgagcgccca gcctagcccc gtcgcgcgca tcgcgacggg
      601 atacgtcatc gacgcaagcg cggtagcgcc aacttgcgcg ccgatgacgc cggtgccggc
      661 cagcatcagc acggccgtgg tcggccactg cgtcaagtcc gtcgccgtcg cgaacgtgta
      721 gaagccgacg gcggcgaacg cgaggccgac gagcaccatg cgatgcgcgc cgaggcgatc
      781 catcagcaag ccgatcgcgt aggtgccgat cacgccgccg acgtgataca gcgtcgtcat
      841 gacggaggcc tgcgtccgcg aaaagcccga cgcttcgagc gacgtcggca gccagttgat
      901 cgcaaggtag acgttcaaca ggctcatgaa gaaggcgacc cagaggcaga cggtcgcgat
      961 gccgcggccg tcgctgaaga gagcgcgtag cgtggctttg ccgccctcgg gatcgtgcga
     1021 aatgatggcg gcgtccgcgc cgatctccgg cttgatcata cgtaggagcc ggttggctgc
     1081 gctttggcgg ctcgggatca gcgccgcgaa gcggatcgac tccggcaacc agcgcgcgag
     1141 cgccgcggcg agcagcagag gcagcacgcc gcccacgacg aacatcgatt cccaaccgta
     1201 cggcccgacg aagcgagccg ccgccgcgcc ggcgccgatc gcgccgagcg atatgccgct
     1261 gccgacgaac atgacgatca acgcgcggcg ccggtgcggc gcgtattccg acgcgagccc
     1321 gatcgcattc ggcagcgcgg cgccgagccc caagcccgtg aagaagcgca gcgcaactag
     1381 cggtagcagc gagccgacgg ccggcgtcag cagcgtgagc acgccgaagg cgaggcacga
     1441 gtaaacgatg actttgcggc gcccgactcg atcggcgagc ggcgcaagaa acatcgcgcc
     1501 gagcatcacg ccgatgagcc cgcccgtcag cgcaggccac aattgcccgc gcacgagccc
     1561 ccattcgctg gtgagcgtcg gcacgacgta ggtgatgcct tgcacgtcga agccgtcgac
     1621 gaacagcacc gccgcgcaca gcaggaacac gcgcagctgg aacgagccga cggcgctcgt
     1681 gtcgatgacg tccgaaacgt tgatggctcg agtcacgtgc tgcggccgtg gattggcgac
     1741 tgaagttgcc tcaatcataa acgactgtga ccgatctcgc tttccgcatg cgcgttccct
     1801 cactaatatg ccgtcggcat ctccggcgac tgtcgcgcca tccacgaggc caacgccatg
     1861 caggaccgca atccctacgc cgcaccgcaa acgaacgtca cgcaaggcga cggccccgtc
     1921 gaatacgggg aagtgaaaat tttctcggtg agcggccgcc tcggccgagt ccgctacatc
     1981 ggttattcgg tcggcgtcag cttgctgatc atgctcggcc tcggaatcgt cgctgcgatc
     2041 gtaggcgccg gcgacgtcaa caggatgttc ctggttggct tagccggcta cgcgctcatc
     2101 ctcgtcgtgc agctcatgct gacgattcag cgcgcgcacg acatgaacac gacgggttgg
     2161 ctcgcgctga tctggttcgt tccgctcgcc cagttggtgt tctggctcgt gcccggcaca
     2221 cgcggcgaga acgattacgg caagcagccg ccgcccaata ctatcggcgt gatagtgctg
     2281 gcctgcgtcg ttccgttggt attcgtcggc ggcattttgg cggcaattgc cattccggcg
     2341 tatcaggatt acacgatccg cgcgcaggtc agcgaagggc tgagcctggc cgccgggatc
     2401 aaggcggccg tcgcggaggc gttcgcgcgc acggaatccg caccgcggga tcgcctcgag
     2461 gccggtctac ccgaggatgc gactgacacc tcggggcagt acgtgacgag cgtcgatatc
     2521 gccgacggca cgatcctcat caattacggc gcgaacgctc actcgacgat agccggtgcc
     2581 gtgctcgcgc tgcagccgta cgtcatgccc gacaacagcg tcgtgtggcg ctgcggcgag
     2641 gccgccgagc cgcccggcgc agcggccctg gacggcagtg cgttgagctc cgacgaggcc
     2701 acggacatcg ggccgcgcta cttgccgtcc gcttgtcgcc cgtgacggca cgcggtctgt
     2761 aacgaaccgc atgtgaggca agtcgcagtt cctcggcggc gtctcaagta acgcatgacg
     2821 cggccgaccc cgatgaggtt ggccaagcga gcgtcacgat gccggcaccc gcacaaactg
     2881 ctgcttcgaa cgacccgacc cgggagctcg ccgcgctacg cgccgagtgc gagcagctgc
     2941 gcgagctctc gacgcgccgg agtcaggaat tcgaagagcg caccgccgag ctcgcgcgca
     3001 cggcgaag
//
LOCUS       DATLEE010000220         6878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2488630, whole genome shotgun sequence.
ACCESSION   DATLEE010000220 DATLEE010000000
VERSION     DATLEE010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2488630"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1995)
                     /gene="aceE"
                     /locus_tag="VM692_04710"
     CDS             complement(<1..1995)
                     /gene="aceE"
                     /locus_tag="VM692_04710"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HUQ51499.1"
                     /translation="MATARPLPLIALRGDDFDPEETQEWLESIDSVLRVHGAERAHFL
                     LECMIEHARRSGAYLPYSPNTAYLNTIPVGQQPEYPGDRSIERRIEAYIRWNAMAMVV
                     HANRISSEYGGHIASYASSATLYEVGFNHFWRAPTAEHGGDLVYFQGHSSPGIYARAF
                     LEGRLDENNLNHFRQEVAGPGVGLSSYPHPWLMPEFWQFPTVSMGLGPMMAIYQARFM
                     RYLESRGIVPAQDRKVWCFLGDGETDEPESMGAITMPVRERLDNLVFVINCNLQRLDG
                     PVRGNGKIIQELEAAYQGAGWNVIKVVWGSRWDPLLARDEKGLLRKTMEECVDGEYQA
                     FKAKGGAYTREHFFGRYPELKAMVAHLSDEDIWHLNRGGHDPVKVFAAYNAAVNHKDR
                     PTVILAKTVKGFGMGEAGEGQNITHQKKKLDDDDLKHFRDRFHVPISDEELAALKYYK
                     PSPDSEEMRYLKERRGKLGGFLPARRLKAEPLQVPQLEFFKSQLEGSGDREFSTTMAF
                     VRILTALVRDKNIGPRIVPIVPDEARTFGMEGMFRQVGIYSSLGQLYTPQDSDQLSYY
                     KEDKKGQILEEGINEGGAYCSWIAAATSYANHGVQMIPFYIYYSMFGFQRIGDFAWAG
                     GDMRARGFLLGGTAGRTTLAGEGLQHQDGHSQLVSTTVPNC"
     gene            2184..3707
                     /locus_tag="VM692_04715"
     CDS             2184..3707
                     /locus_tag="VM692_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HUQ51500.1"
                     /translation="MIELPDFAPLRVTATTAAPTRTTLDGLDHLLVVTARRGESNLAR
                     LPYGKPLGPLLARATKNGDEFASSRAANKRATGLTIATFGAAAGFAALSWAGKAIREC
                     LRDKPKTLGVAFAGLEDAAAHTAAASLAAAASAAAFALPEFKSPSKTPRPPRLATLRL
                     FGPAGIVDTAGVRAQALGNNVARWFTALPPNVLTIAGYRQAIEALANPRGISCRFIAE
                     PELEKLGAGAFLAVARGNATRDAGIMHLSYRPRGRETARVALVGKGVIFDTGGTNLKT
                     FTNMLDMHTDMQGSAVAFGTLLALAELQVPFGIDAWLAITENRLAADAYKSQDLVTAL
                     NGTTIQVIHTDAEGRMVLADTLALAAREQPALIIDYATLTGTCVMALTERYSGAFSNR
                     AAANALLLAAGTASGERVWPFPMDDDFDELLKSDIADIKQCSAESAGDHILGARFLSR
                     FVPKEIPWIHLDLSASQHKGGLAHVPTDITGFGVRLTLELLTRATTGPGDLAKRLDV"
     gene            3704..4366
                     /gene="rnt"
                     /locus_tag="VM692_04720"
     CDS             3704..4366
                     /gene="rnt"
                     /locus_tag="VM692_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HUQ51501.1"
                     /translation="MSQEEATVTVAEAPVSPIAARFRGFLPVIIDVETGGFNCATDAL
                     LEIAAVFVRFNAAGDLEPGERHRYIVKPFEGSRLDPASLQVTGIDPFHPLRPAVDEAE
                     ALRALFQEVRKEIKPSGCTRAVLVGHNSFFDLQFLNAAVERTGIKRNPFHPFSSFDTA
                     TLAGVAYGQTVLSRAVTAAGFEWQDESAHSALYDADMTARLFCSVVNRFRSVYEGSTT
                     GQ"
     gene            complement(4353..4679)
                     /gene="grxD"
                     /locus_tag="VM692_04725"
     CDS             complement(4353..4679)
                     /gene="grxD"
                     /locus_tag="VM692_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HUQ51502.1"
                     /translation="MDVNERIKKQLESYPVLLYMKGTPDFPQCGFSAKTVGVLRAAGA
                     KFAYINIFEDPEIREGLKAYSNWPTFPQLYVNGELIGGCDIAVEMFESGELQKVLSEA
                     APATAQ"
     gene            4849..5433
                     /locus_tag="VM692_04730"
     CDS             4849..5433
                     /locus_tag="VM692_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HUQ51503.1"
                     /translation="MNPLNTIQGTIIAGVVLAVVIALATMGINPNELSMIVWLHVMAG
                     ITWIGLLYYFNFVQVPALAAAAKDQGGPGGAGISKYVAPRALLWFRWGAVFTWLTGAL
                     YLLRVGLLDDAFMLRGPAMTIGVGAWLGTIMLFNVWVLIWPNQKKVLGLVEATADQIA
                     KARRVAFLASRTNTLLSVPMLMSMVGFGHGGFFL"
     gene            5520..6368
                     /gene="nadC"
                     /locus_tag="VM692_04735"
     CDS             5520..6368
                     /gene="nadC"
                     /locus_tag="VM692_04735"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HUQ51504.1"
                     /translation="MAPPPLPADIATVVSRAIAEDLRDGDLTAELIARTTQAVAHVVA
                     REPATLCGRAWFDETFRQIDARVAVTWNAADGAQVGADTIVCELRGPARSIVTGERTA
                     LNLLQTLSGTATVTRTYAELVAGTRTRILDTRKTLPGLRLAQKYAVRCGGGENHRIGL
                     FDAVLIKENHIAAVGSVSAAVAAARRQSGNVMIEVEVESLTQLREALATDADRVMLDD
                     FSLDDMRAGVQLRDAHGGKRKELEASGSVDAAKLEAVAATGVDFISIGAITKHVRAID
                     FSMRFS"
     gene            complement(6548..>6878)
                     /locus_tag="VM692_04740"
     CDS             complement(6548..>6878)
                     /locus_tag="VM692_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ51505.1"
                     /translation="NRFIQALKELGCKFALDDFGTGLSSFGYLKHFPVDFLKIDGSFV
                     KEILHDPIDREMVRSINEIGHLTGKQTIAEFAENTEIITMLRGMGIDYAQGYGVSEPK
                     RLLQAVA"
BASE COUNT         1210 a         2250 c         2168 g         1250 t
ORIGIN      
        1 gcaattcggc accgtggtcg agaccagctg actgtgtccg tcctgatgct gcaagccctc
       61 gcccgccaac gtcgtgcgcc ccgccgtgcc gccgagcaga aaaccgcggg cgcgcatgtc
      121 gccgcccgcc cacgcgaaat cgccgatgcg ctggaaaccg aacatcgagt aatagatata
      181 gaaaggaatc atctgcacgc cgtggttggc gtacgacgtc gcggccgcga tccacgagca
      241 atacgcgccg ccctcgttga tgccttcctc gaggatctgc cctttcttgt cttccttgta
      301 ataagacagc tgatccgaat cctgcggcgt gtagagctgg cccagcgacg aatagatgcc
      361 gacttggcgg aacatgcctt ccatgccgaa ggtgcgcgcc tcgtcgggca cgatgggtac
      421 gatgcgcggg ccgatgttct tgtcgcgcac gagcgccgtc aaaatgcgca cgaacgccat
      481 cgtcgtcgag aactcgcgat cgccgctgcc ttcgagctgg ctcttgaaga actcgagctg
      541 cggcacctgc agcggctcgg ccttgagccg ccgcgccggt aggaacccgc cgagcttgcc
      601 gcgacgttcc ttcaagtacc gcatctcctc gctgtccggc gagggcttgt agtacttcag
      661 cgccgcgagc tcctcgtcgg agatcggcac gtggaagcga tcgcggaagt gcttcaggtc
      721 gtcgtcgtcg agcttcttct tttgatgcgt gatgttttgg ccttcaccgg cctcgcccat
      781 accgaagcct ttcaccgtct tcgcaagaat gacggtcggc cgatccttat ggttgacggc
      841 cgcgttgtac gccgcgaaca ccttcacggg atcgtggccg ccgcggttca agtgccagat
      901 gtcctcgtcg gacagatgcg cgaccatcgc cttgagctcg gggtagcgcc cgaagaaatg
      961 ctcgcgcgtg taggcgccgc ccttggcctt gaacgcctgg tactcgccgt cgacgcactc
     1021 ttccatcgtc ttgcgcagca gccctttctc gtcgcgcgcg agcagcgggt cccagcgcga
     1081 gccccagacg accttgatga cgttccagcc cgcgccctga taagcggcct cgagctcctg
     1141 aatgatcttg ccgttgccgc gcacgggacc gtcgaggcgc tgcagattgc agttgatgac
     1201 aaagacgaga ttgtcgaggc gctcgcgcac cggcatcgtg atcgcgccca tcgactcggg
     1261 ctcgtcggtc tcaccatcgc ccaagaagca ccagaccttg cgatcttgcg ccggcacgat
     1321 gccgcgactc tcgaggtagc gcatgaagcg cgcctgatag atcgccatca ttgggcccaa
     1381 gcccatcgac accgtcggaa actgccaaaa ctcaggcatc aaccacgggt gcggatacga
     1441 cgacagcccg acgccgggtc ccgcgacttc ctgacggaag tggttcaggt tgttctcgtc
     1501 gaggcgccct tcgagaaacg cgcgcgcata gatgcccgga gacgaatgac cctggaagta
     1561 cacgaggtcg ccgccgtgct cggccgtcgg cgcgcgccag aaatggttga agccgacttc
     1621 gtacaaagtc gccgacgagg cgtagctcgc gatgtggccg ccgtactcgg agctgatgcg
     1681 gttcgcgtgc acgaccatcg ccatcgcgtt ccagcggata tacgcctcga tgcggcgctc
     1741 gatcgagcga tcgccgggat actcgggctg ctgcccgacg ggaatcgtat tcagatacgc
     1801 cgtgttcggg ctgtacggca gatacgcgcc cgagcggcgc gcgtgctcga tcatgcattc
     1861 gagcaggaag tgggcgcgtt cagcaccgtg gacccgcagc acggaatcga tcgactcgag
     1921 ccactcctgc gtctcttcgg ggtcgaagtc atcgccgcgg agagcgatca acggcagcgg
     1981 tcttgcagtc gccatgagaa accgtactcc agttacgctg tggttaagct cgggctcttc
     2041 ggactccgtc aatcctgcta ggatgcgagc ccgttcgctt agccacctga cccccgctct
     2101 agcggcgcta tgatcgggta tttgtagaaa gacggtcaag gcaaagggat ccgcgtggcg
     2161 aagctccgat cgatggtcag ccgatgatcg aactacccga tttcgcgccg ctgcgcgtca
     2221 cggcaacaac ggccgcgcct acgcgaacga cgctcgacgg cctcgatcac ctgctcgtcg
     2281 tcacggcgcg ccgcggcgaa tcgaatcttg ctcgtttgcc ctacggcaaa ccgctcggtc
     2341 cgctgcttgc tcgcgcaacc aagaacggcg acgagttcgc cagctcgcgt gcggccaaca
     2401 agcgagcaac cggcttgacg attgccacgt tcggcgctgc ggcggggttc gcagctctga
     2461 gctgggccgg aaaagcgatc cgcgaatgcc tgcgcgacaa accgaagacg ctcggcgtgg
     2521 cattcgcggg cctcgaggac gcggcggcgc acacggccgc ggcaagtctc gcggctgccg
     2581 ccagtgccgc ggcttttgcg ctgccggaat tcaaatcgcc gagcaagacg ccgcgcccgc
     2641 cacggctcgc gactctgcgt ctgttcggcc ctgccggcat cgtcgacacg gccggcgtac
     2701 gcgcacaagc cttgggcaac aacgtcgcgc gttggttcac tgcattgccg ccgaacgtat
     2761 tgacgatcgc cggctaccgt caggcgatcg aggcgctcgc gaacccgcgc ggcatcagct
     2821 gccgtttcat cgctgagccc gagctcgaga aacttggcgc cggggcgttc ttggcagttg
     2881 cgcgcggcaa tgcgactcgc gatgccggca tcatgcactt gagctaccgg ccgcgcggcc
     2941 gcgagaccgc gcgtgtcgcg ctcgtgggca aaggcgtgat cttcgacacg ggcggcacga
     3001 atctgaagac gttcacgaac atgctcgaca tgcacacgga catgcagggc agcgccgtcg
     3061 cgttcggcac attgctcgcg ctcgcggagc tgcaagtgcc attcggcatc gatgcctggc
     3121 ttgcgatcac cgagaatcgg ctggccgccg acgcctacaa gtcgcaggac ctcgtgacgg
     3181 ccctcaacgg cacgacgatc caggtcattc atacggacgc cgaagggcgc atggtcctcg
     3241 ccgacacgct ggcactcgcg gcacgcgagc agcccgcgct gatcatcgat tacgccacgc
     3301 tgacgggcac ctgcgtcatg gcgctgacgg agcgctactc gggagcgttc tcgaatcgcg
     3361 ccgcggccaa cgcattgctg ctggcggccg gcactgccag cggcgagcgc gtctggccgt
     3421 tccctatgga cgacgacttc gacgagctgc tgaagagcga catcgccgac atcaagcaat
     3481 gcagcgccga gagcgccggc gatcacatct tgggcgctcg cttcctgtcg cggttcgtgc
     3541 cgaaagagat tccgtggatc cacctcgact tgagtgccag ccagcacaag ggcggcttgg
     3601 cgcacgtccc gacggacatc acgggcttcg gagtacgctt gacgctcgag ctgcttacgc
     3661 gtgcaacgac gggccccggc gacctagcga agcggctcga cgtatgagcc aggaagaagc
     3721 gacagtgacg gttgccgaag cccccgtctc gccgatcgcg gcgcgtttcc gcggcttcct
     3781 gcccgtcatc atcgacgtgg aaacaggcgg cttcaattgc gcgacggatg cgttgctcga
     3841 gatcgccgcc gtgttcgtgc gcttcaacgc cgcgggggac ctcgagcccg gcgagcgcca
     3901 ccgctacatc gtcaagccgt tcgaagggtc acggctcgat cccgcctcgc tgcaggtgac
     3961 gggcatcgac ccgtttcatc cgttgcgtcc cgcggtcgac gaggccgaag cgctgcgcgc
     4021 gctgtttcag gaagtgcgca aggagatcaa gccgtcgggc tgcacgcgcg ccgtgctcgt
     4081 gggccacaac tcgttcttcg acttgcagtt cctgaatgcc gcggtcgagc ggaccggcat
     4141 caaacgcaat ccgttccacc cattctcgag cttcgacacg gcgacgctcg ccggcgtcgc
     4201 gtacggccaa acggtattga gccgcgccgt tacggcggcg ggattcgagt ggcaagacga
     4261 gagcgcgcac tcggcgctct acgacgccga tatgactgcg cggctgtttt gctcggtcgt
     4321 aaatcgcttc cgcagcgtct acgaaggctc gactactggg cagtagcggg tgcggcttcg
     4381 ctgagtacct tctgcagctc gcccgactcg aacatctcca cggcgatgtc gcagccgccg
     4441 atcagctcgc cgttgacgta cagctgcgga aacgtcggcc agttcgaata cgccttgaga
     4501 ccctcgcgaa tctcggggtc ctcgaagatg ttgatgtaag cgaacttcgc gccggccgcg
     4561 cgcagcacgc cgacggtttt cgccgagaac ccgcactgcg gaaaatccgg agttcccttc
     4621 atgtacagca acacgggata ggactcgagt tgcttcttga tgcgttcgtt gacgtccatg
     4681 gtgcgaattc ctcggttggc gacgcgaagt caagccgcgc gattgcggca ggctcgttga
     4741 actgcgtctg tgtctcacct attcttcttg agtcttcgaa gcggggcaag gcccgcgccg
     4801 accgacaaga acgcaagaat taggtcaagt ccgtcagggg gaggtctcat gaacccattg
     4861 aataccattc aaggcaccat cattgccggt gtcgtgctcg ccgttgtgat tgcgctcgcc
     4921 acaatgggca tcaacccgaa cgagctgagc atgatcgtct ggctacatgt catggccggc
     4981 atcacctgga tcggcttgct gtattacttc aatttcgtgc aggtgcctgc cctcgcggct
     5041 gccgccaagg atcaaggcgg ccccggcgga gccgggatca gcaagtacgt agccccgcgt
     5101 gcgctgctgt ggttccgctg gggtgccgtg ttcacgtggc tcacgggcgc gctgtatctg
     5161 ttgcgcgtcg gcctcctcga cgacgcgttc atgctgaggg gccccgcgat gacgatcggc
     5221 gtcggcgcgt ggctcggcac gatcatgctg ttcaacgtct gggtattgat ttggccaaac
     5281 cagaaaaaag ttttgggtct cgtcgaagcg acggcggatc agattgccaa agcgcgtcgc
     5341 gtcgccttcc tcgcgtcgcg aaccaacacg ttgttatcgg tcccgatgct gatgagcatg
     5401 gtcggcttcg gtcacggcgg cttcttcctg tagccgccgc gcgcaaaccg ctcgactcga
     5461 ggacccggcg aagcgcgagc tttccgggtc cttttcgttt ccgaggagtg agctagccaa
     5521 tggcgccgcc cccgcttccc gccgacatcg caaccgtcgt gagccgcgcg atcgcggagg
     5581 accttcgtga cggcgacctc accgccgagc tcatcgcgcg gacgacacag gccgtcgcgc
     5641 acgtggttgc tcgcgagccg gctacgctgt gcggccgcgc ttggttcgac gaaacgtttc
     5701 ggcagatcga tgcgcgagtt gccgttacgt ggaatgcggc cgacggcgcg caggtcggcg
     5761 cggacacaat cgtctgcgag ctgcgcggcc cggctcgcag catcgtgacc ggcgagcgca
     5821 cggcgctgaa cttgcttcag acgctgtcgg gcacggcgac cgtgacccgc acttacgccg
     5881 agctcgtcgc aggcacccgc acgcgcatcc tcgacacgcg caagacgctt ccgggtttgc
     5941 gcctcgcgca gaaatacgcc gtgcgttgcg gtggcggcga gaaccaccgc atcggcctgt
     6001 tcgatgccgt gctgatcaaa gaaaaccata tcgcggccgt cggcagcgtc tcggccgctg
     6061 tcgcagccgc gcgccggcag tccggcaacg tgatgatcga agtcgaggtc gaaagcctga
     6121 cgcagctacg cgaagcgctc gcgaccgacg ccgaccgcgt catgctcgac gacttctcgc
     6181 tagacgacat gcgcgccggc gtgcagttgc gcgacgcgca tggcggcaag cgcaaggagc
     6241 tcgaggcttc cggcagcgtc gatgctgcga agctcgaggc cgtcgcggcg acgggcgtcg
     6301 acttcatctc gatcggcgcg atcacgaagc acgtacgcgc gatcgacttc tcgatgcgct
     6361 tctcctagcc gctagctcgg gattgcggac gcccgccgcg agctatttcc gatcgcgttc
     6421 gtagcgcgcg accagcggta aagcgggcaa ccagctgcga tgacgcgaac gttggcgcag
     6481 aggcagccgc ccgtgaatcg gtccatctct ttgactgaag cggacggaaa ccgccggctg
     6541 cgaaattcta tgcgacggcc tgcagcaggc gcttcggctc cgacacgccg tagccctgcg
     6601 cgtagtcgat gcccatacct cgcagcatcg tgatgatttc ggtgttttcg gcgaactcgg
     6661 cgatcgtctg cttgcccgtc aagtgtccaa tctcgttgat cgaccgcacc atctcgcgat
     6721 cgatcgggtc gtgcaggatc tctttgacga agctgccgtc gatcttcaag aagtcgacgg
     6781 ggaaatgctt caggtaaccg aatgacgaga ggccggtgcc gaagtcgtcc aacgcgaact
     6841 tgcagccgag ctccttcagc gcctggatga agcggttg
//
LOCUS       DATLEE010000221         2439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2489658, whole genome shotgun sequence.
ACCESSION   DATLEE010000221 DATLEE010000000
VERSION     DATLEE010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489658"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VM692_04745"
     CDS             <1..890
                     /locus_tag="VM692_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HUQ51506.1"
                     /translation="EALRELAPLHNPPALAVARATATRFTHAPLYAVFDTAYFADLPP
                     HVRELALPPEWRFGRGLRRFGFHGLAHEYLAREARKLPGRRRRLITLQLGNGCSAAAV
                     LDGKPIDTSMGFSPLEGLIMATRPGDLDAGVLLELARRGESWSTISHGLNREAGLKGL
                     SGASSDVRELLELEAHGHAGATLAMAAFHYRITKYIGAYTAVLGGLDAIAIGGGIGEN
                     APAVRARICRDLEWLGVRLDAAANDDAVGAASRISQPASVATVDVIPVDEETLIAEHV
                     RDALPVAQQANESGARSAS"
     gene            887..>2439
                     /locus_tag="VM692_04750"
     CDS             887..>2439
                     /locus_tag="VM692_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HUQ51507.1"
                     /translation="MSEPLDAAALGVLDAYWRAANYLAVGQIYLQANPLLEQPLTLAH
                     VKPRLLGHWGTTPGLNLIYAHLNRVIKARDLPMLLVIGPGHGGPALVANTWLDGSYSD
                     VYPDVPRDKEGMQRLFKQFSFPGGISSHCSPEIPGSIHEGGELGYSLAHAFGAAFDNP
                     QLVVACVIGDGEAETGALAASWHSNKFLHPTNDGAVLPILHLNGWKIANPTLLARIPE
                     QELSELMRGYGWEPHFVSGHEPMPVHSAMAATLDSALDEIAAIKHAQDRGRSTMPRWP
                     MIVLRTPKGWTGPHEVDGDAVEGTWRAHQVPLAALAERPEHLLELERWLKSYRPAELF
                     DSAGAPRDELLTQVPSGALRLGATPYANGGLLRKNLRLPDYRDYAVAVPRPGAVHAEA
                     TRVLGGYLRDVLVENGAARNFLVFGPDETASNRLAKIYEATGKRHLGPMLDVDRNLAT
                     DGRVFEVLSEHLCEGWLEGYLLTGRHGFFSCYEAFVHIVDSMFNQHAKWLKVTNKEIA
                     WRRPIASLN"
BASE COUNT          389 a          836 c          820 g          394 t
ORIGIN      
        1 tcgaagcgct gcgcgagctc gcgcctttgc acaatcctcc ggccctcgcc gtcgcgcgcg
       61 cgacggcgac gcgcttcacg cacgcgccgc tctacgccgt gttcgacacg gcgtacttcg
      121 cggacttgcc gccgcacgtg cgcgagttgg cgcttccccc cgaatggcgg ttcggccgcg
      181 gcctgcggcg attcggcttt catggcctcg cgcacgagta cctcgcgcgc gaggcgcgca
      241 agctgcccgg ccgacggcgg cggctcatta cgctgcagct cggcaacggt tgttccgcgg
      301 ccgccgtgct cgacggcaag cccatcgaca cgagcatggg attctcgccg ctcgaaggct
      361 tgatcatggc tacccgaccc ggcgacctcg atgccggtgt gctgctcgag ctcgcgcgtc
      421 gcggcgagtc ctggtcgacg atcagtcacg gcctgaatcg cgaggccggg ctcaaagggc
      481 tgtcgggcgc gagcagcgac gtgcgcgagc tactcgagct cgaggcgcac ggccatgccg
      541 gcgcgacgct ggcgatggcc gcgttccact atcggatcac gaaatacatc ggcgcatata
      601 ccgccgtcct cggcggtctc gacgcgatcg cgatcggcgg cggaatcggc gagaacgcac
      661 cggccgtgcg cgcgcgcatc tgccgcgacc tcgagtggct tggtgtacgg ctcgacgcgg
      721 ccgcgaacga cgacgccgtc ggcgcggcga gccgcatctc gcagcccgcg tcggttgcga
      781 ccgtcgacgt catcccggtg gacgaggaga ccctgatcgc cgagcacgtc cgcgatgcgc
      841 tgcccgtcgc gcaacaggcg aacgaaagcg gcgctcggag cgcttcttga gcgagccgct
      901 cgacgcagcc gcgctcggcg tgctcgacgc gtactggcgc gccgcgaatt accttgccgt
      961 cggccaaatt tacttgcagg caaacccgct gctcgagcag ccgctgacgc tcgcgcacgt
     1021 gaagccgcgg ctgttgggtc attggggcac gacgccgggc ttgaatctga tctacgcgca
     1081 tttgaatcgc gtcatcaagg cgcgcgacct gccgatgctg ctcgtcatcg gccccggcca
     1141 cggtggaccg gcgctcgtcg cgaacacgtg gctcgacggc agctactcgg acgtttatcc
     1201 cgatgtgccg cgcgacaagg aaggtatgca gcggctgttc aagcagttct cgtttcccgg
     1261 cggcatctcg agccattgct cgccggagat tccggggtcg atccacgaag gcggcgagct
     1321 cggctattcg ctcgcgcacg cgttcggcgc cgcgttcgac aacccgcaac tcgtcgtcgc
     1381 ctgcgtgatc ggcgacggcg aagccgagac cggcgcgctc gccgcgagct ggcactcgaa
     1441 caaattcttg cacccgacga acgacggcgc cgtattgccg atcctgcatc tgaacggctg
     1501 gaagatcgcc aacccgacgc tgttggcgcg cataccggag caggagttga gcgagctcat
     1561 gcgcggctac ggctgggagc cacacttcgt ttccggtcat gagcccatgc ccgtgcacag
     1621 cgcgatggcc gcgacgctcg actcggcgct cgacgagatc gccgcgatca agcacgcgca
     1681 agatcgcggt cgcagcacga tgccgcgctg gccgatgatc gtgttgcgga cgccgaaggg
     1741 ctggacgggc ccgcacgagg tcgacggcga cgccgtcgaa ggcacgtggc gcgcgcatca
     1801 agttccgctg gcggcgctcg ccgagcgccc cgagcacttg ctcgagctcg agcggtggct
     1861 caagagttat cgccctgccg agctgttcga ctccgcgggc gcgccgaggg acgagctgct
     1921 cacgcaggta ccgagcggag ctctgcggct cggcgcgacg ccgtacgcga acggcggtct
     1981 gctgcgcaaa aacctgcggc tgcccgacta ccgcgactac gccgtcgccg tgccgcgccc
     2041 cggcgccgtg cacgccgaag cgacaagagt gcttggcggc tatctacgcg acgtgctcgt
     2101 cgagaacggc gcggcgcgta acttcctggt gttcggcccc gacgagacgg catcgaatcg
     2161 gctggcgaaa atctacgagg caaccggcaa acggcacttg ggccccatgc tcgacgtcga
     2221 tcgcaatctg gcgactgacg gccgcgtgtt cgaggtcttg tcggagcacc tctgcgaggg
     2281 ctggctcgaa ggctatctcc tgacgggacg ccacggcttc ttctcgtgct acgaggcgtt
     2341 cgtgcacatc gtcgattcga tgttcaatca gcacgcgaaa tggctcaagg tgacgaacaa
     2401 ggagattgcc tggcggcgcc cgatcgcttc gttgaatta
//
LOCUS       DATLEE010000222         2694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_999988, whole genome shotgun sequence.
ACCESSION   DATLEE010000222 DATLEE010000000
VERSION     DATLEE010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999988"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VM692_04755"
     CDS             <1..183
                     /locus_tag="VM692_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51508.1"
                     /translation="PLSEIGDQFAFSQLHPWHFEAACEWLERKGLVEKVSAPFKLTKR
                     SLERVEEPAYFLDTGR"
     gene            191..>2694
                     /gene="uvrA"
                     /locus_tag="VM692_04760"
     CDS             191..>2694
                     /gene="uvrA"
                     /locus_tag="VM692_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUQ51509.1"
                     /translation="MRTTIEIRGARENNLQNIDVEIPRDKLVVVTGVSGSGKSSLAFD
                     TVYAEGQRRLLASMSTFAKRFVGQLKKPDVDFVSGLSPVVSIDQKTVGSNPRSTVGTM
                     TDVSDYLRMLFATMGTPHCPLCNEALAIRTPHQMMEHILSLPKGTGVEVRAPVYKIHG
                     EDWEFLFELIRVNGYRSARIDGKPRDLGEHIELGEDEPHTVEAVVDSFVVGPDIDRQV
                     VTSLEHGLKLGDGLLGFHIVKPKSLTPAHKKFYDGFGCSEHRLVAGTMEAYQFTFNDP
                     AGACPTCAGIGTAMRVHPKLLVPDPARTLNEGAFVNAALGNSPDSWGGRMLFSLAARY
                     GFSLDVPFQDLAQKHVDILFYGTKGEQFEVKVPPKAKVGQQHAGKKIKFDGVVTQLEH
                     NYRRYRKQGMSNAGMDEYLKSVMVEYDCPECGGARLKRTRRLVTIDERNLYEVGEMHM
                     ADLLKFLRGIKPTARQRAIAETILREVATRLELLVAIGLDYLSLNRRSATLSGGESQR
                     IRLSSQIGSGLMGMLYVLDEPSIGLHPKDNVKMIETLQRLRDLGNTVIVVEHDGDTIR
                     AADHILEIGPGPGKHGGKIVAEGPLSKILKDGNSLTGQYLSGKKRIDLPARRRPASGK
                     SLVVRGARENNLKGIDVEIPLEQFVCVTGASGSGKSSLIHDIVQKRLYSLFHDSRVFA
                     GAHDELEGAEHLSDVIDVDQSPIGRSSRSNPATYIGFYDAIRSLFAATEQAKQRGFTA
                     STFSFNVKGGRCEECSGEGTITTQLSFMPDVEVLCPTCKGARYNQDTLEVEYVGKNIA
                     QVLDLSVEEGVKFFADEPVIARKIAVLDELGLGYLTL"
BASE COUNT          511 a          864 c          872 g          447 t
ORIGIN      
        1 ccgttgtcgg agatcggcga tcagttcgca ttctcgcagc tgcacccgtg gcacttcgag
       61 gcggcctgcg aatggctcga gcgcaaaggc ctcgtcgaga aagtctcggc gccgttcaag
      121 ctgacgaagc gcagcctcga gcgtgtcgag gagccggcct acttcctcga caccggccgc
      181 tgaggcgctg atgcggacaa cgatcgagat tcgcggcgcc cgcgagaaca acctgcagaa
      241 catcgacgtc gagattccgc gcgacaagct cgtcgtcgtc acgggcgtca gcggctcggg
      301 caagtcgtcg ctggcgttcg acaccgtgta cgccgaaggg caacggcgcc tcctcgcgtc
      361 gatgtcgacg ttcgcgaagc gcttcgtcgg ccagctcaag aagcccgacg tcgacttcgt
      421 cagcggtctc tcgcccgtcg tgtcgatcga tcagaagacc gtcggctcga atccgcgctc
      481 gacggtcggc acgatgaccg acgtctccga ctacttgcgc atgctgttcg cgacgatggg
      541 gacgccgcac tgtccgctgt gcaacgaggc gctcgcgatc cgtacaccgc accagatgat
      601 ggagcacatc ctgtcgctgc cgaagggcac cggagtcgag gtacgggctc cggtctacaa
      661 gattcacggc gaggattggg aattcctgtt cgagctgatc cgcgtcaacg gttaccgcag
      721 cgcacgtatc gacggcaagc cgcgggatct cggcgagcat atcgagctcg gcgaggacga
      781 gccgcacacg gtcgaagccg tcgtcgactc gttcgtcgtc gggccggaca tcgaccggca
      841 agtcgtgacg tcgctcgagc acgggctcaa gctcggcgac ggcttgctcg gctttcacat
      901 cgtcaagccg aagtcgctga cgccggcaca caagaagttc tacgacggct tcggctgctc
      961 ggagcatcgg ctcgtcgcag gcacgatgga agcctatcaa ttcacgttca acgaccccgc
     1021 gggagcgtgc ccgacgtgcg ccggcatcgg cacggcgatg cgcgtgcatc cgaagctact
     1081 cgtgccggac cctgcgcgca cgttgaacga gggcgcgttc gtcaacgccg cgctcggcaa
     1141 cagccccgac agctggggcg gacgcatgct cttcagcctc gcggcgcgct acgggttcag
     1201 cctcgacgtg ccgtttcagg accttgcgca gaagcacgtc gacatcctgt tctacggcac
     1261 gaaaggcgag cagttcgagg tcaaggtgcc gccgaaggcg aaagtcggcc agcagcacgc
     1321 cggcaagaag atcaagttcg atggcgtcgt gacgcagctc gagcacaact atcgccgcta
     1381 ccgcaagcaa ggcatgtcga acgccggcat ggacgagtac ttgaagagcg tgatggtcga
     1441 gtacgactgc cccgagtgcg gcggcgcgcg cctaaagcgc acgcggcggc tcgtcacgat
     1501 cgacgagcgc aatctctacg aggtcggcga gatgcacatg gccgacctgt tgaagttctt
     1561 gcgaggcatc aagccgacgg cgcgccagcg cgcgatcgcg gagacgatcc tgcgcgaggt
     1621 tgcgacgcgc ttggagttac ttgtagcgat cggcctcgac tacctgagcc tcaatcgccg
     1681 ctccgcgacg ctgtcgggcg gggagtcgca gcgcatccgc ctgtcgtcgc agatcggctc
     1741 gggcctgatg gggatgctgt acgtgcttga cgagccgagc atcgggctgc accccaaaga
     1801 caacgtcaag atgatcgaga cgctgcagcg cctgcgtgat ctcggcaaca cggtgatcgt
     1861 cgtcgagcac gatggcgaca cgattcgcgc cgccgaccac atcctcgaga tcggtcccgg
     1921 ccccggcaag cacggcggca agatcgtggc cgaaggcccg ttaagcaaga tcttgaagga
     1981 cggcaactcg ctgacgggtc agtacttgag cggcaagaag cgcatcgacc tgccggcgcg
     2041 gcggcgcccg gcgagcggca agtcgctcgt cgttcgcggc gcccgcgaga acaatttgaa
     2101 gggcatcgat gtcgagattc cgctcgagca attcgtctgc gtgacgggcg cgtcgggctc
     2161 gggcaagagc tcgctgattc acgacatcgt gcagaagcgc ctctattcgc tgttccacga
     2221 tagccgcgtc ttcgcgggcg ctcacgacga gctcgagggc gccgagcact tgagcgacgt
     2281 catcgacgtc gaccaatcgc cgatcggccg ctcctcgcgc tcgaatccgg cgacctacat
     2341 cggcttctac gacgcaattc gctcactgtt cgcggcgacc gagcaagcga agcagcgcgg
     2401 cttcacggcg tcgaccttca gcttcaacgt caaaggcggc cgctgcgagg agtgcagcgg
     2461 cgagggcacg atcacgacgc agctgtcgtt catgcccgac gtcgaggtcc tgtgcccgac
     2521 gtgtaagggc gcgcgctaca accaggacac gctcgaggtc gagtatgttg gcaagaacat
     2581 cgcccaagta cttgatctat cagttgaaga aggcgtcaaa tttttcgcgg acgagcccgt
     2641 catcgcgcgc aagatcgccg tgctcgacga gctcggcttg ggctacctca cgct
//
LOCUS       DATLEE010000223         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_256988, whole genome shotgun sequence.
ACCESSION   DATLEE010000223 DATLEE010000000
VERSION     DATLEE010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256988"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VM692_04765"
     CDS             complement(<1..753)
                     /locus_tag="VM692_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUQ51510.1"
                     /translation="MSGKRDLFIGGQWVAAKNKATFPVYNPSTGEVWSQVADASRSDA
                     AAAIAAAAAAQPEWAAMAHSKRAAILSKVADVLTARQKEFQEVLIDEGGAWIGKTMFE
                     TGYSIGVYRAAAAAAYQVTGEILPSDHGKLSLVVREPLGVVSVISPWNFPLILSSRGF
                     ATALAVGNTVVLKPSEETPVSGGILIGEILEEAGVPKGVFNVITCSRDNVGAVGDELI
                     SNPTVRGISFTGSTAVGTQVAAKAGGLLKKCCV"
     gene            966..>2305
                     /locus_tag="VM692_04770"
     CDS             966..>2305
                     /locus_tag="VM692_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HUQ51511.1"
                     /translation="MAGCRALPARGPVVLEEALPVARYGSLADFAGRIEAMLGSGESA
                     HWLLDSNELAMKARLALADEAATSLDVQYFVWQSDATGELLIDRVLHAAQRGVKVRVL
                     MDDFGVAGRGGDVIKLDAHPNIEVRVFNPWSTRGTRFGTATEFLTRAYVLNRRMHNKT
                     FIADGRFAVLGGRNIGDRYFGVYEPFVQNDLDVMVAGPMAREISATFDAFWNSEHSFP
                     AALFERDSRPTHPPEATQQEAHGSNASNAKILASFPLEPTDWSGYLEELVATFAPARS
                     EVLWESPDILDSSRPRLYDEYKALVASARREVLISSPYFIPDEEFRALLRDLVARGVR
                     VVVVTNSLATNNHVVAHTGYRRLRREVLAAGVELYELRADAEALSYSVTPPATSEHLG
                     LHSKAVVVDDERAFVGSPNVDPRSMVLNTEIGIVGEGPVFTARVAALLERDISPA"
BASE COUNT          367 a          751 c          765 g          422 t
ORIGIN      
        1 gacgcagcac ttcttgagca ggccgccggc cttggcggca acttgggtgc cgacggccgt
       61 cgagcccgtg aagctgatgc cgcgcaccgt ggggttcgag atcagctcgt cgccgacggc
      121 gccgacgttg tcgcgggagc acgtgatgac gttgaagacg cctttcggaa caccggcctc
      181 ttcgaggatc tcgccgatca ggatgccgcc ggagacgggc gtttcctccg acggcttcag
      241 cacgacggtg ttgccgacgg ccaaggccgt ggcgaagccg cgcgacgaca ggatgagcgg
      301 gaagttccac ggcgagatca cggacacgac gccgagcggc tcgcgcacga cgagactcag
      361 cttgccgtga tccgacggca ggatctcgcc ggtcacctga taggcggctg cggcggcggc
      421 gcgataaacg ccgatgctgt agccggtctc gaacatcgtc ttgccgatcc aagcgccgcc
      481 ttcgtcgatc aagacttctt ggaattcttt ttgccgcgca gtcaacacgt cggcgacctt
      541 cgacaagatc gccgcgcgct tcgagtgtgc catcgcggcc cactctggtt gcgcggccgc
      601 ggcggcggca attgcggcgg ccgcgtcgct gcggctcgcg tccgcgactt gcgaccacac
      661 ttcgccggtc gaagggttgt agacagggaa cgtcgctttg ttcttggccg cgacccactg
      721 gccgccgatg aaaagatctc gcttgccgct catgagtttt ctcctcgacc gaatcgacga
      781 tgaatctttt tgtggacggc gcgaagcgcc ggtcggccca tccctcgttg ttgcttgcaa
      841 tgggcttcgc cgcgctagta tagacaacga gtggggtcga taaccaaagc acacgccgaa
      901 cgcgtacgag tcgtcgtgac gtgatccggg ctgcgtgtct cctggtcgtt ctctgtaccg
      961 taacggtggc gggctgtcgg gcgctgccgg cccgcggtcc tgtcgtgctc gaggaagccc
     1021 tgccggttgc tcgttatggt tccctggcgg attttgcggg ccgcatcgag gccatgctcg
     1081 gcagcggcga gtccgctcac tggttgctcg acagcaacga gctcgctatg aaagcgcgcc
     1141 tcgcgctcgc cgacgaggca gcgacctcgc tcgacgtgca gtatttcgtc tggcagagcg
     1201 acgccacggg agagctgctg atcgaccgtg tcctgcacgc cgcgcagcgc ggggtcaagg
     1261 tccgagtcct catggacgat ttcggcgtcg ctgggcgtgg cggcgacgtc atcaagctcg
     1321 acgcgcatcc caatatcgaa gtccgggtgt tcaacccctg gtcgacgcgc ggcacgcgct
     1381 tcgggaccgc aacggagttc ctcacgcggg catacgttct gaatcgccgc atgcacaaca
     1441 agacgttcat cgccgacggg cgcttcgcgg tgctcggcgg gcgcaacatc ggcgaccgtt
     1501 atttcggcgt gtatgaaccg ttcgtgcaga acgatctcga cgtcatggtc gccgggccga
     1561 tggcgcgcga gatcagcgcc accttcgacg ccttctggaa cagcgagcac tcgtttccag
     1621 ccgcgttgtt cgagcgcgat tcccggccga cgcatccgcc ggaagccacg cagcaggaag
     1681 ctcacggcag caacgcaagc aacgcaaaaa tactcgcgtc gtttccgctc gagccgacgg
     1741 actggtcggg ctatctcgaa gagctcgtcg cgacgttcgc gccggcgcgc agcgaggtgc
     1801 tctgggagtc gccggacatc ttggactcct ctcggccgcg gctctacgac gagtacaagg
     1861 cgctcgtcgc gagcgcgcgg cgcgaggtct tgatcagctc gccgtacttc attcccgacg
     1921 aggagtttcg tgcgctactg cgtgacctgg tcgcgagggg cgtgcgcgtg gtcgttgtca
     1981 cgaactcgct cgcgaccaac aatcacgtgg tcgcccacac gggttatcgg cgcttgcggc
     2041 gcgaggtgct tgcagccggc gtcgagctct acgagctgcg cgcggatgcc gaggcgctgt
     2101 cctactccgt gacgccgccg gccacgtctg agcaccttgg cttgcattcg aaagccgtcg
     2161 tcgtcgacga cgagcgggcg ttcgtcggct cgccgaacgt cgacccgcgc tcgatggtgc
     2221 tcaacacgga gatcggcatc gtcggcgaag gcccggtctt cacggcgcgc gtcgcggcgc
     2281 tgctcgagcg cgacatctcg ccggc
//
LOCUS       DATLEE010000224         2186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8325860, whole genome shotgun sequence.
ACCESSION   DATLEE010000224 DATLEE010000000
VERSION     DATLEE010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8325860"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="VM692_04775"
     CDS             complement(<1..1301)
                     /locus_tag="VM692_04775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ51512.1"
                     /translation="MTTPPAASPSSSEPDAGGYPRYVLAVLVVVYVLNFLDRQILSIL
                     NEHIKADLGLTDAQMGFLFGTAFAVFYALFGIPLGRLADVWTRRTLISLGLAFWSAMT
                     ALSGLAQNFPQLAGARIGVGIGEASASPAAYSLLSDWFPPRRRATVLAIYSSGIYIGA
                     GCGLMIGGQIVDRWDAAFPAGTAPYGLRGWQTAFFAVGLPGLALAAWVRTLREPPRGG
                     ETAVSHGSPFSEFLRELRAVLPPLTLWTMATEGGAPTLVRNIGAAAAIAAVAWALTAW
                     SGDAAQWGALGIGAYAAASWVQALQIRSPEAADVILGTPTLRWASLGFGFLAFTGYAA
                     GYWGAPYFMRVHHVPASEVGFVVGGVAAVAGWLGVSAGGALADAWRTRSMHGRLYTGM
                     VTAALTPPLLAAMLAAPSTNVAFVVNAPLQFVSSMWIGAGIA"
     gene            1320..>2186
                     /locus_tag="VM692_04780"
     CDS             1320..>2186
                     /locus_tag="VM692_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ51513.1"
                     /translation="MSAPSEASRRVVCDACPLCGATSWDAFVDGGPQGLEWVRCACGA
                     VFKHREPASVQMAEPASVEKAEPASVEKAEPASVEMAEPASVGTAERASAGQPEPASS
                     HYDEAYFTRYERRRRRRIAKSRRQILDALDVAPPGRLLDVGCSLGYTLEAARTLGLDA
                     AGVDLSAHAVAECLRRGFDARRGGLDDLPFNDASFALAILKHVFEHTPSPRRTLTELR
                     RVLVPGGAIFLAVPNLEYFKASRSPETSRFFRGEGGEAHYVYYTPGTLSRLLEEEGFA
                     VRSVHARLVHRRA"
BASE COUNT          377 a          737 c          763 g          309 t
ORIGIN      
        1 gcgataccgg cgccgatcca catcgacgag acgaactgca acggcgcgtt cactacgaac
       61 gcgacattcg tgctgggtgc ggcgagcatt gcggcgagga gcggcggcgt aagcgctgcg
      121 gtgaccatgc cggtgtacag gcggccgtgc atcgagcgcg tacgccacgc gtcggcgagc
      181 gcgccgcccg cgctcacgcc gagccatccg gcaacggcag cgacgccgcc cacgacgaat
      241 ccgacttcgg aagccggcac gtggtgcacg cgcatgaagt acggcgcgcc ccaatagccg
      301 gccgcgtatc ccgtgaacgc gaggaagccg aagccgagcg acgcccagcg cagcgtcggc
      361 gttccgagga tcacgtccgc cgcttcgggg cttcgaatct gcagagcctg cacccacgag
      421 gccgcagcgt aggctccgat gccgagcgcg ccccactgcg cggcgtcacc gctccacgcc
      481 gtgagtgccc acgcgaccgc ggctatcgca gccgcggcgc cgatgttgcg gacgagcgtc
      541 ggtgcgccgc cctcggtcgc cattgtccac agcgtgagcg gcggcagcac ggcgcgaagc
      601 tcgcgcagga attcggaaaa aggagatccg tgcgaaacag cggtttcgcc gccgcgcggc
      661 ggctcgcgaa gtgtgcgaac ccaggccgcg agcgcgagcc ccggaagccc aacggcaaag
      721 aacgctgttt gccagcctcg cagcccatac ggcgccgttc ccgcgggaaa cgccgcatcc
      781 cagcgatcga cgatctggcc gccgatcatc aggccgcagc ccgctccgat gtagatgccg
      841 ctcgaataga tcgcgaggac ggttgcgcga cggcgcggcg ggaaccagtc ggacagaagc
      901 gaatacgcgg ctggtgaagc gctcgcttcg ccgatgccga cgccgatgcg cgcgcccgcg
      961 agctgcggaa aattctgcgc gaggccggac aacgccgtca tcgcactcca gaacgcaagg
     1021 ccgagcgaga tgagcgtgcg cctcgtccaa acgtcggcga gtcgcccgag cggaatgccg
     1081 aacagcgcat agaagactgc aaaggccgtc ccgaacagga agcccatctg tgcgtccgtc
     1141 agtccgaggt cggccttgat gtgctcgttc aggatcgaaa ggatctggcg gtcgaggaag
     1201 ttgaggacgt atacgacgac gagcacggcg agcacgtagc gcggatagcc accggcgtcg
     1261 ggttcggacg aggacggact cgcggcgggc ggcgtcgtca tggtatgggg cgaacctgca
     1321 tgagcgcgcc ctctgaagcc agtcgccgcg tcgtctgcga cgcgtgtccg ctttgcggcg
     1381 cgacgtcgtg ggacgcgttc gtcgacggcg gcccgcaagg gttggagtgg gtgcgctgcg
     1441 cctgcggcgc ggtgttcaag catcgcgagc ccgcgagcgt tcaaatggct gagcccgcga
     1501 gcgttgaaaa ggccgagccc gcgagcgtcg aaaaggctga gcctgcgagc gttgaaatgg
     1561 ccgagcccgc gagtgttgga acggccgagc gtgcgagcgc tggacaaccc gagccggcca
     1621 gcagtcacta cgacgaagcg tacttcactc gttacgagcg gcgccggcgt cgccgcatcg
     1681 cgaagtcacg gcgccagatc ctcgacgcgc tggatgtcgc gccgccggga cggctactcg
     1741 atgtcggttg ctcgctcggc tacacgctcg aggcggcccg cacgctcggg ctcgatgcag
     1801 cgggggtcga tctctcggcc cacgccgtcg ccgaatgcct gcggcgcggc ttcgatgcgc
     1861 gtcgcggcgg tctcgacgat ttgcctttca acgatgcgag cttcgcgctc gccatcctca
     1921 agcacgtttt cgagcacaca ccgtcaccgc gccggacact gaccgagctg aggcgcgtgc
     1981 tcgtacccgg cggtgcgatt ttcctcgccg ttccgaatct cgagtacttc aaggcgtcgc
     2041 gttcgccgga gacttcccga ttcttccgcg gcgagggcgg cgaggcgcac tacgtgtact
     2101 acacgccggg cacgctctcg cggctgctcg aagaggaagg gttcgccgtg cggagcgtgc
     2161 acgcccggct cgtgcaccgt cgcgcg
//
LOCUS       DATLEE010000225         2015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3979457, whole genome shotgun sequence.
ACCESSION   DATLEE010000225 DATLEE010000000
VERSION     DATLEE010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3979457"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..881)
                     /gene="xth"
                     /locus_tag="VM692_04785"
     CDS             complement(108..881)
                     /gene="xth"
                     /locus_tag="VM692_04785"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HUQ51514.1"
                     /translation="MKIATFNVNGISARLPRLLEWLAETAPDIVCLQELKAPPERFPA
                     LALQAAGYEALWHGQKSWNGVAILAKELPVERRRVLPGDAADSHSRYLEAEVAGLVVA
                     CLYVPNGNPAPGPKFSYKLRWLDRLRRHADELLASGAPVVLAGDFNIIPTDLDVYAPE
                     RWRDDALFRPEVRAAFEQLVASGWIDALRTLYPDERVYTFWKYLGNAFTRDAGLRIDH
                     LLLTPAGATRLQKAGVDKHVRGRDKASDHAPVWIDLVRK"
     gene            complement(878..>2015)
                     /locus_tag="VM692_04790"
     CDS             complement(878..>2015)
                     /locus_tag="VM692_04790"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HUQ51515.1"
                     /translation="DEPYARARDSRVRAALAETGSDFRTRKDHVVFERGELLTATGAP
                     FSVFTPYKNAWLKKLSPADLAPHAVAENAQRLAPPPADRRGVPALESIGFAATNVLEH
                     LVPGTRGAERLVQDFLARIDDYAETRNYPAVKGPSYLSVHLRFGTVSVRALARAAHER
                     MLAGSKGAEVWLSELIWRDFYHQVLYHHPHVVEGAFRREYDALQWEQGAHAEQFLAAW
                     QEGRTGYPLVDAAMRQLNQTGYMHNRLRMLTASFLTKDLGVHWRDGERYFALQLNDFD
                     LAANNGGWQWAASTGTDAQPYFRIFNPVTQSEKFDAQGDFIRRYVPELTQLSPATIHA
                     PWLADSAELAAAGVRLGETYPRPIVDHAAARKRTLARYAAVKAQ"
BASE COUNT          326 a          669 c          685 g          335 t
ORIGIN      
        1 tgagttgtgc atagacgtag ttgcggatac gcggacgttt gactcgctca atccaaagct
       61 ttccgcagca aaaaagccac tcggcgctcg ccggccgagt ggcttgttca tttcctcacg
      121 agatcgatcc aaacgggcgc gtgatcgctg gccttgtcgc ggccgcggac gtgtttgtcg
      181 acgcctgctt tttgcaatcg cgtcgcgcca gccggcgtca acaacagatg gtcgatgcgc
      241 agaccggcgt cgcgcgtgaa cgcgttgccc aagtatttcc agaacgtgta gacgcgctcg
      301 tcgggataaa gcgtgcgcaa tgcgtcgatc caaccgcttg caacgagctg ctcgaacgcg
      361 gcgcgcactt cggggcgaaa cagcgcgtcg tcacgccaac gctccggcgc gtacacgtcg
      421 aggtccgtcg ggatgatgtt gaagtcgccc gcgagcacga cgggcgcgcc gctcgcgagc
      481 agctcgtccg cgtgccgccg caagcggtcg agccaacgta gcttgtaaga aaatttcggc
      541 cccggagccg gattaccgtt cggcacatac agacacgcga cgacgaggcc ggccacttcg
      601 gcttcgagat agcggctgtg actgtccgcg gcgtcgccgg gcagcacgcg gcgccgctcg
      661 acgggcaact ccttcgccaa gatcgcgacg ccgttccagc tcttctgtcc gtgccacagc
      721 gcctcgtaac ccgcggcttg cagcgccagc gcgggaaagc gctctggcgg cgccttgagc
      781 tcttgcaggc acacgatgtc gggcgcggtc tcggcgagcc attcgagcag ccgcggcaag
      841 cgcgcgctga tgccgttgac gttgaacgtc gcgattttca ttgcgccttc acggcggcgt
      901 agcgcgcgag cgtgcgcttg cgcgccgcgg cgtgatcgac gatcggccgc ggatacgtct
      961 cgccgagccg cacgccggct gccgcgagct cggcggagtc ggcgagccaa ggcgcgtgga
     1021 tggtcgccgg cgaaagctgg gtgagctccg gcacgtaacg gcgaatgaag tcgccttggg
     1081 cgtcgaactt ctcgctctgc gtcacggggt tgaagatgcg gaaatacggc tgcgcgtccg
     1141 tgcccgtcga cgcggcccac tgccagccgc cgttgttcgc agcgagatcg aaatcgttga
     1201 gctgcagcgc gaaatagcgc tcgccgtcgc gccagtggac gccgagatcc ttcgtgagaa
     1261 agctcgccgt cagcatgcgc aggcggttgt gcatgtaccc cgtctgattc agttggcgca
     1321 tcgccgcatc gacgagcgga taacccgtgc gaccttcttg ccacgccgcg aggaactgct
     1381 cggcgtgcgc gccctgctcc cactgcaaag cgtcgtactc gcggcggaac gcgccctcga
     1441 cgacgtgcgg atggtggtac agcacttggt ggtagaagtc gcgccagatg agctccgaaa
     1501 gccagacttc ggcgcccttc gagcccgcga gcatgcgctc gtgggccgcg cgcgcgagcg
     1561 cgcgaaccga gacggtgccg aaccgcaggt gcacgctcaa gtagctcggg cctttgacgg
     1621 ccggatagtt gcgcgtctcg gcgtagtcgt cgatgcgcgc gaggaaatcc tgcaccagcc
     1681 gttcggcgcc gcgagtaccc ggcacgagat gctcgagcac gttcgtcgcg gcgaagccga
     1741 tgctctcgag cgccggcacg ccgcggcgat cggcaggcgg tggcgcgagc cgctgcgcgt
     1801 tctcggcgac cgcgtgcggc gccaggtccg ccggcgacaa cttcttgagc cacgcgttct
     1861 tgtacggcgt gaagacgctg aacggcgcgc ccgtcgccgt cagcagctcg ccgcgctcga
     1921 agacgacgtg atccttgcgc gtgcggaagt cgctgccggt ctcggccagc gccgcgcgca
     1981 cgcgcgagtc gcgcgcccgt gcatacggct cgtcg
//
LOCUS       DATLEE010000226         5045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3981033, whole genome shotgun sequence.
ACCESSION   DATLEE010000226 DATLEE010000000
VERSION     DATLEE010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3981033"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="VM692_04795"
     CDS             <1..1599
                     /locus_tag="VM692_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUQ51516.1"
                     /translation="AFVWLSNRYQGALRAMLRAPWVAVAGCLAIAFGAYLLLQEIPQE
                     YAPVEDRGQFNGQIQAPEGTSYERLRESAMKVEAALQKYFDDGTVQRGIVSVPGFGNN
                     GSGIVNVTLKPYGERKIGTAEMLADLNKQWEEIPDVRVNAFMGGFNRGGGGGGGGGSP
                     VQLVLGGPNYEELARWRDIIIARASENPGLLRLDSDLRETQPQVLVRVDQDRAASLGV
                     TARSIGTTLQTLMSERQVTTYVVDGEEYDVVLQAKPEQRASYSDLSNIFVRADRTGEL
                     IPLSNLMTLEDMAGPSSLQRHNRMRAITLSANLAPGYTLGEALDYLENIVRTELPDTA
                     QIDYRGESLEYKEASGALYFTFGIALFVVFLVLAAQFESFVHPLVIMVTVPLAVAGGL
                     LGLWFTGKTLNIYSQIGIIMLVGIAAKNGVLIVEFINQLRDKGLEFKEAIVEASRIRL
                     RPVIMTAFAAVMGSVPLILAEGPGSASRAALGVVIFSGVMFATFFTLFIVPAVYNLMA
                     RRTSSPNAIAHRLDEMRVEPEPSL"
     gene            1892..2014
                     /locus_tag="VM692_04800"
     CDS             1892..2014
                     /locus_tag="VM692_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51517.1"
                     /translation="MAANGYYVMLRPLPPGKHTINFGGALPTNVQLVTYTLIVE"
     gene            complement(2020..2703)
                     /locus_tag="VM692_04805"
     CDS             complement(2020..2703)
                     /locus_tag="VM692_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HUQ51518.1"
                     /translation="MPFVIFGILVLVLAVLPGVWVQSVMRRYRQPANRYPRTGGQTAR
                     QLLDAIGLRNVATEITEHGDHYDPMAKVVRLSKENFNGRSLTAVTVAAHEVGHALQDA
                     RGFGPLHWRTRLVHWVQPIEKVGAGMLLLTPLAGVLMRSPYLGLIGLAGGILTLGAGV
                     AVHFLTLPTELDASFARALPLLKNQGVLRREDLPRARLLLTAAAFTYVAVALQSLLNI
                     ARWWAILRR"
     gene            complement(2720..3589)
                     /locus_tag="VM692_04810"
     CDS             complement(2720..3589)
                     /locus_tag="VM692_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HUQ51519.1"
                     /translation="MRLLSFAVGDYRSFGAVNGDGIVDLGRLMGEPYPTLGSVLRAGD
                     LHRAISLGATSKPMISLEEIRYLPTVPDPEKIVCIGINYGNRNEEYKDGADAPKYPSV
                     FMRTPGSLVGHLEPILNPPESEQLDYEGEIVLVIGKAGRRIAAGDAWQHVAGLSLMNE
                     GSVRDWLRHSKFNVTPGKNFERSGSLGPWLVTADELAPSAELHLTTHVNGELRQDDTT
                     ANLIFPFARLIEYLSSFMRLMPGDLIATGTPTGAGARFDPPKYLRGGDTVEVHVPELG
                     TLRNAVVDETTAS"
     gene            complement(3596..4498)
                     /gene="dapA"
                     /locus_tag="VM692_04815"
     CDS             complement(3596..4498)
                     /gene="dapA"
                     /locus_tag="VM692_04815"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HUQ51520.1"
                     /translation="MPQRRHPLAALRGSIPPLITPFKRGEVDYDRYAQLVEFQVQQGT
                     HGILVNGTTAEPSTLTTDERNRLVTLAVEVTKGRVPVVAATGSQSLAESRALTEHALQ
                     AGADSLLIVTPYYIRPPQRGLVAYYLELAAGHDVPWMIYHIPGRTAVSVTLDTLKACK
                     DKSPHFVGIKQAVNDLGFVSECLHEFGHDFKVFVGLEELSFPMMTIGACGLMNAVGNL
                     RPKVLAQMCEAVWSGDMAAGQRLHEQLLEINKAVFYDTNPIPMKYMMKKLGLLDDNEH
                     RLPMISATPELAAKLDGVLERAGL"
     gene            4587..4982
                     /locus_tag="VM692_04820"
     CDS             4587..4982
                     /locus_tag="VM692_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HUQ51521.1"
                     /translation="MPHLIVEYSANIEDQLALDALLDKLHATALATGVFPIGGLRVRA
                     HRTAAYRIADLRAENGFVHVTAMIGQGRPLDVQQRAAEELFAALTAHLEPLYAKSPLA
                     ISLNMQEFPVLNFKKNNLHEYVKRRAGKS"
BASE COUNT          846 a         1639 c         1663 g          897 t
ORIGIN      
        1 gcgttcgtgt ggctgtcgaa ccgctatcag ggtgcgctgc gcgcgatgct gcgcgctccg
       61 tgggtggctg ttgcgggctg tctcgcgatc gcgttcggcg cttacctctt gctgcaggag
      121 attccgcagg aatatgcgcc cgtcgaggac cgcgggcagt tcaacggcca gatccaggcg
      181 cccgagggca cgagctacga gcggctacga gaatcggcga tgaaggtcga ggccgcgctg
      241 cagaaatact tcgacgacgg caccgtccag cgcggcatcg tcagcgtgcc ggggtttggc
      301 aacaacggct cgggtattgt caacgttacg ttgaagccgt acggcgagcg caagatcggc
      361 acggccgaga tgcttgccga tctcaacaag cagtgggaag agatcccgga cgtgcgcgtc
      421 aacgcgttca tgggcggctt caatcgcggt ggtggcggcg gtggtggcgg cggctcgccg
      481 gtgcagctcg tcctcggcgg cccgaactac gaggagctcg cgcgctggcg cgacatcatc
      541 atcgcgcgcg cgtcggagaa ccccggcctc ctccgcctcg attcggattt gcgcgagacg
      601 cagccgcaag tgctcgtgcg cgtcgaccag gaccgtgccg cgagcctcgg cgtcaccgcc
      661 cgcagcatcg gcacgacgct gcagacgctg atgagcgagc ggcaggtcac gacctacgtc
      721 gtcgacggcg aagaatacga cgtcgtcctg caagcgaaac ccgagcagcg cgccagctac
      781 tccgacttga gcaacatctt cgtgcgcgcg gatcggaccg gcgagctgat cccgctctcg
      841 aacctcatga cgctcgagga catggcgggc ccgagctcgc tgcagcggca caaccgcatg
      901 cgggcgatca cgctgtccgc gaacctcgcg ccgggctata cgctcggcga ggcgctcgat
      961 tacctcgaga acatcgttcg caccgagctg cctgacacgg cgcagatcga ctaccgcggt
     1021 gagtcgctcg agtacaagga agcgtcgggc gcgctgtatt tcacgttcgg catcgcgttg
     1081 ttcgtcgtgt tcctcgtgct ggcagcgcag ttcgagagct tcgtgcatcc gctcgtcatc
     1141 atggtcacgg tgccgctggc cgtcgcaggc gggttgctgg gactctggtt cacgggcaag
     1201 acgctgaaca tctatagcca gatcggcatc atcatgctcg tcggcatcgc ggccaagaac
     1261 ggcgtattga tcgtcgagtt catcaatcaa ttacgcgaca aaggcttgga gttcaaagag
     1321 gcgatcgtcg aggcgtcgcg cattcgtctg cggcccgtca tcatgacggc cttcgcggcc
     1381 gtcatgggct ccgtgccgct gatcctcgcc gaaggcccgg gctcggcgag ccgcgcggcg
     1441 ctcggcgtcg taatcttctc gggcgtgatg ttcgcgacgt tcttcacgct gttcattgtg
     1501 ccggcggttt acaacctcat ggcgcgccgg acgagctcgc cgaacgcgat cgcgcatcgg
     1561 ctcgacgaga tgcgcgtcga gccggagccg agcctctagc gttcgcgcaa ttggcggggg
     1621 cgcgcggttc tgtgaacccc gccgagacgg cgtaggcgca gtcacgaaag aatccgccgt
     1681 atgccgcgtt ctccctattg cttcgccgcg ctgttgctcg tcgcgtgctc ggcctcggcc
     1741 gaggagacgt atctcgttcc gccggatgag atcgtagccg aggtgtcgca ggaggactgg
     1801 tcgcgcactt ggtggcaccg agcatcgcca ggccacgcgg caatgcttcg acctcggaga
     1861 gctgacgaag ccacggctcg agctctatcc cgtggcggcc aacggctact acgtgatgct
     1921 gcgcccgctg ccccccggta agcacacgat caactttggc ggcgcgttgc cgaccaacgt
     1981 gcagctcgtg acgtacacct tgatcgtcga atagcgccgt cagcgccgca ggatcgccca
     2041 ccagcgcgcg atgttcagca ggctctgcag cgcgacggcg acgtaggtga acgctgccgc
     2101 cgtcagcagc aggcgagcgc gcggcagatc ttccctgcgc agcacgccct gattcttcaa
     2161 aagcggcaga gcgcgcgcga agctcgcatc gagctccgtc ggcaacgtca ggaagtgcac
     2221 ggcgacgccg gcgcccagcg tcaggattcc tccggcgaga ccgatgagcc cgagataagg
     2281 cgaccgcatc agcacgcccg cgagcggcgt cagcagcagc atgcccgcgc cgaccttctc
     2341 gatcggctgc acccaatgca cgagccgcgt gcgccagtgc agcgggccga agccgcgtgc
     2401 gtcctgcaac gcatggccga cctcgtgcgc ggcgaccgtc acggccgtca gcgagcggcc
     2461 gttgaaattc tccttcgaca ggcgcacgac cttcgccata gggtcgtagt gatcgccgtg
     2521 ctcggtgatc tcggtcgcga cgttgcgcag gccgatcgca tcgagcaact ggcgcgcggt
     2581 ttggccgccc gtgcgtggat agcgattcgc cggctggcgg tagcggcgca tgacgctctg
     2641 cacccaaacg cccggcagca ccgcgagcac caacaccaag atgccgaaaa tcacgaaggg
     2701 cattgccctg cctcgcggtt cagctcgccg tcgtctcgtc gacgacggcg ttgcgcagcg
     2761 tgccgagctc gggcacgtgg acctcgacgg tatcgccgcc gcgcaagtac ttcggcggat
     2821 cgaagcgcgc gccggcgccc gtcggcgtgc ccgtcgcgat gagatcgccg ggcatcaggc
     2881 gcatgaagct cgagagatac tcgatgagcc gcgcgaacgg gaagatcaaa ttcgccgtcg
     2941 tgtcgtcctg acgcagctcg ccgttgacgt gcgtcgtcag gtgcagctcc gcgctcggcg
     3001 ctagctcgtc ggccgtgacg agccacggcc ccaagctgcc cgaacgctcg aagttcttgc
     3061 ccggcgtgac gttgaatttc gagtggcgca gccaatcgcg gaccgaccct tcgttcatca
     3121 gcgacaagcc ggcgacgtgt tgccacgcgt cgccggccgc gattcgtcgg cccgccttgc
     3181 ctatcacgag cacgatctcg ccttcgtagt cgagctgctc ggactcgggc ggattcagga
     3241 tcggctcgag gtgcccgacg agactgcccg gcgtgcgcat gaacacgctg ggatacttcg
     3301 gcgcgtccgc gccgtctttg tattcttcgt tgcgattacc gtagttgatg ccgatgcaga
     3361 cgatcttctc gggatcgggc acggtcggga ggtagcggat ctcttcgagc gaaatcatcg
     3421 gcttgctcgt cgctcccagc gagatcgcgc ggtgcaagtc gccggcgcgc aggacgctgc
     3481 cgagcgtcgg atagggctcg cccatgaggc gcccgagatc gacgatgccg tcgccgttga
     3541 cggcgccgaa gcttcgatag tcgccgaccg cgaagctcag cagtcgcatg gcgcgctaca
     3601 ggcccgcgcg ctcgagcacg ccgtcgagct tggcggcaag ctccggtgtc gcgctgatca
     3661 tcggcagccg gtgctcgttg tcgtcgagca ggccgagctt cttcatcatg tacttcatcg
     3721 ggatcgggtt cgtgtcgtag aacacggctt tgttgatctc gagcagctgc tcgtgcagcc
     3781 gctggcccgc ggccatgtcc cccgaccata cggcctcgca catttgtgcg agcaccttcg
     3841 gccgcaaatt gccgacggcg ttcatgagcc cgcacgcgcc gatcgtcatc atcgggaagc
     3901 tcagctcctc gagcccgacg aaaactttga agtcgtggcc gaactcgtgc aggcattccg
     3961 agacgaaccc gagatcgttg acggcttgct tgatgccgac gaagtgcggc gacttgtctt
     4021 tgcaggcttt caacgtgtcg agcgtgacgc tgacggccgt gcggccgggg atgtggtaga
     4081 tcatccacgg cacgtcgtgg cccgcggcga gctccaagta ataggccacg agaccgcgtt
     4141 gcggcgggcg gatgtagtag ggcgtcacga tcagcagcga gtcggcgccg gcctgcaacg
     4201 cgtgctcggt cagcgcgcgg ctctcggcca gcgactgcga gcccgtcgcg gccacgacgg
     4261 ggacgcggcc cttcgtgact tcgactgcga gcgtcacgag ccgattgcgc tcgtcggtcg
     4321 tcagcgtcga cggctcggcc gtcgtgccgt tgacgagaat gccgtgcgtg ccttgctgga
     4381 cctgaaactc gacgagctgt gcgtaacgat cgtaatcgac ttcgccgcgc ttgaacggtg
     4441 ttatgagcgg aggaatcgag ccgcgcagtg cagcgagcgg atgtcgtctt tgcggcattt
     4501 cgtgtctcaa attgacagtc gttgcgaccg tcaattaccg tgtgaaatcg ctttcgagtg
     4561 tacgttaagc cgtctcggga ttcaccttgc cgcacctgat cgtcgagtat tcggccaaca
     4621 tcgaggatca gcttgcgctc gatgcactgc tcgacaagct ccacgcgacc gcactcgcga
     4681 ccggcgtctt tccgatcggc ggcctgcgcg tgcgcgcgca tcgcacggct gcttaccgca
     4741 tcgcggactt gcgtgccgag aacggtttcg tccatgtcac ggcgatgatc ggccagggcc
     4801 ggccgctcga cgtgcagcag cgtgctgccg aggaactgtt cgcggcgctg acggcgcatc
     4861 tcgagccgct gtacgccaag agcccgctcg cgatctcgct gaacatgcag gagttccccg
     4921 tgctgaactt caagaagaac aacctgcacg agtacgtgaa acggcgcgca ggcaagtcat
     4981 gagcggcgcg ggctcgttcg aaggcaacgt cgcgaaggcc gagcaattca tcgcgcgctt
     5041 tcgca
//
LOCUS       DATLEE010000227         3097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9199968, whole genome shotgun sequence.
ACCESSION   DATLEE010000227 DATLEE010000000
VERSION     DATLEE010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9199968"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..2337)
                     /locus_tag="VM692_04825"
     CDS             complement(61..2337)
                     /locus_tag="VM692_04825"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HUQ51522.1"
                     /translation="MSEDRRRIPEFYKLSVEERVRAVHERGLINTADFRALATGEHTL
                     GLDSADKMIENVVGVMGMPLGLGMNLVVNEQQYIVPMVVEEPSVVAALGSACKLIRAC
                     GGFKAEATDPILIGQIQILNLPDMEKAKQALRERRNDIINLANSLHPNMVARGGGARD
                     IELFVHPLPSSAKQMLVVHLLVDTRDAMGANLVNGMCEGVAPLVESITGGEVFLRILS
                     NLADRSVVRASCKIPVEQLAGKGYTGVQARDGIIVAGEFAAVDPYRAATHNKGIMNGI
                     DPIAIATGNDWRAIEAGAHAYAARGGRYSSLTEWWADEAGNLCGRIELPIKVGIVGAP
                     IESNPTVALNLRLLGVNSAVELAQVMGAAGLAQNFAAIRALATEGIQKGHMTLHARSV
                     VTAAGAPKEIFDEVLERLIQSGVIKVWKAQELVAEVQDERHKAAAGPRRKRSKEAAMG
                     VGYGKVILLGEHAVVYGRHAIAAPIPLAIKALVEDCDEGIHLLIPRWNVEYRLASNPN
                     ERRSFERPAGALLDHLGLSKRAMKIEVFPEIPRSMGLGGSAAMAVAIVRALDKHFRLK
                     LKDDEVNRLAFESEKLAHGNPSGLDNTLACYGKALVFRAGDPPLVEPLNIREPIPAVI
                     GMTGLEGLTAKTVGRVREAWQQDNKLYERIFDQIDALTLRGIQAIQDNDLPTLGQLMN
                     ICQGMLNALQVSTPELEKVCAIARENGALGAKLTGGGGGGSIIAICDGDTEPVVSAIR
                     AAGFQALPVHLGADLNGA"
     gene            complement(2334..>3097)
                     /locus_tag="VM692_04830"
     CDS             complement(2334..>3097)
                     /locus_tag="VM692_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51523.1"
                     /translation="HATIDSRALFAGSEKLGLGSSAAVVCAWAAACAAFAREQGPGDR
                     ANLRVDELIALHRALQGGKGSGLDVAASYTGGAITFRLTGAGLPQIGSVRLPNSVGFA
                     GIFAGRSASTPGLVAHYRAWQRDRPREAAALVGRLAALAEAGSRALGEGDAEAWLVAF
                     AAYGRGLEELGEAIGADIVTAEHREIGAEAQRHGVAYKVSGAGGGDLGLACALDSERL
                     EAFRTSVGDRGFRVINVSLAEHGLVVAQRNPGTAP"
BASE COUNT          501 a         1074 c          974 g          548 t
ORIGIN      
        1 atctctcggt cgaggtcatc gacgagaatc agctgctcgc ttgcgttcga gacgatctct
       61 tcacgcgcca ttcaaatccg ctccgagatg caccggcagg gcctgaaagc cggccgcgcg
      121 aatcgcgctg accacaggct ccgtatcgcc gtcgcagatt gcaatgatcg agccaccgcc
      181 cccgccgccc gtcagtttcg cgcccagcgc cccgttctcg cgggcgatgg cgcacacttt
      241 ctcgagctcc ggcgtggata cctgcaaggc attcaacatg ccttggcata tattcatgag
      301 ctgaccaagc gttggcaagt cattgtcttg tatggcttgt ataccgcgca acgtcagggc
      361 gtcgatttga tcgaaaatcc gctcatataa cttattgtct tgttgccaag cttcgcggac
      421 gcggccgacg gtcttggccg tcaggccctc gagccccgtc atgccgatca cggccgggat
      481 gggctcccgg atgttcagcg gctcgacgag cggcggatcg ccggcgcgaa acactagcgc
      541 tttgccgtag caggccagcg tgttgtcgag gccgctcggg ttgccgtggg ccagcttctc
      601 agactcgaat gcgaggcgat tgacctcgtc gtccttgagc ttcaagcgga aatgcttgtc
      661 gagtgcgcgc acgatcgcga cggccatggc cgccgagccg ccgaggccca tgctgcgagg
      721 aatttcgggg aacacctcga tcttcatcgc gcgcttgctc aagccgagat gatcgagcag
      781 cgcgccggcg ggccgctcga acgagcggcg ctcgttgggg ttgctcgcga gccggtactc
      841 gacgttccag cgcgggatca acagatggat gccttcgtcg caatcctcca cgagcgcttt
      901 gatcgcgagc gggatcggcg cggcgatcgc atgccggccg tacacgacgg cgtgctcgcc
      961 gagcaggatg accttgccgt agccgacgcc catcgcggct tccttgctgc gcttgcggcg
     1021 cggcccggcc gcggccttgt gccgctcgtc ctgcacctcg gccacgagct cttgagcctt
     1081 ccaaactttg atgacgccgc tctgaatgag gcgctcgagc acctcgtcga agatctcctt
     1141 cggagcgccg gccgccgtca cgacgctgcg cgcgtgcagg gtcatgtggc ctttctggat
     1201 gccttcggtt gcgagcgcgc ggatcgcggc gaaattctgc gccaagcctg cggcccccat
     1261 gacttgcgcg agctcgacgg ccgaattcac gccgagcaag cgcaaattca acgccacggt
     1321 cgggttcgac tcgatcgggg cgccgacgat gccgactttg atcggcagct cgatgcgccc
     1381 gcatagattg ccggcctcgt cggcccacca ctccgtcagc gacgagtaac ggccgccacg
     1441 cgccgcgtac gcgtgcgcgc cggcttcgat cgcacgccag tcgttgcccg tcgcgatcgc
     1501 gatcgggtcg atgccgttca tgatgccttt gttgtgcgtc gcggctcgat acggatccac
     1561 ggccgcgaac tcgccggcga cgatgatgcc gtcgcgcgct tgcacgcccg tgtagccctt
     1621 gcccgcaagc tgctcgacgg gaatcttgca gctcgcgcgc acgacggagc gatcggcgag
     1681 attcgacagg atgcgcaaga acacctcgcc gcccgtgatc gactccacga gcggcgccac
     1741 gccctcgcac atgccgttga cgagattcgc gcccatcgcg tcgcgcgtgt cgacgagcag
     1801 atggacgacg agcatctgct tcgcgctcga cggcagcggg tgcacgaaca gctcgatgtc
     1861 gcgggcgccg ccgccgcgcg ccaccatgtt cgggtgcagg ctgttggcga gattgatgat
     1921 gtcgttgcgg cgctcgcgca gcgcctgctt cgccttctcc atgtccggca gattcaggat
     1981 ttggatctgg ccgatcagaa tcgggtcggt cgcttcggct ttgaagccgc cgcaggcgcg
     2041 aatcagcttg caggccgagc cgagcgccgc gacgaccgac ggctcctcga cgaccatcgg
     2101 cacgatgtat tgctgctcgt tgacgaccag gttcatgccg agcccgagcg gcatgcccat
     2161 gacgccgacg acgttctcga tcatcttgtc ggcggagtcg agaccgagcg tgtgctcgcc
     2221 cgtggcgagc gcgcggaaat cggcagtatt gatgaggccg cgctcgtgaa cggcgcggac
     2281 gcgctcttcg acgctgagct tgtagaactc ggggatgcgg cggcggtctt cgctcatgga
     2341 gccgtccccg ggttgcgttg tgccactacg aggccgtgct ctgccagcga gacgttgatc
     2401 actcgaaaac cccgatcccc aaccgatgtc ctgaatgcct cgagcctttc cgagtcgagc
     2461 gcgcaagcga gcccgaggtc gccgccgccg gcgccgctga ctttgtaagc cacgccgtga
     2521 cgctgcgctt ccgcgccgat ttcgcgatgc tccgccgtga cgatgtccgc gccgatcgct
     2581 tccccaagct cctcgaggcc gcggccataa gcggcgaaag cgacgagcca tgcctcggcg
     2641 tcgccctccc ctagggcccg agacccagct tctgcgagcg ccgccaaacg acccaccagc
     2701 gctgctgcct cccgcggccg atcgcgttgc catgcccggt agtgcgcaac caacccgggc
     2761 gtcgaggcag accggccggc aaaaataccc gcgaatccta cactgttcgg cagtcggact
     2821 gaaccaattt ggggcagccc ggcacccgtc aacctgaacg ttatcgcgcc acccgtgtac
     2881 gacgcggcga cgtcgagccc gctgcccttg ccgccttgca aggccctgtg caacgcgatg
     2941 agctcgtcga ctcgcaaatt cgcgcgatcg ccgggcccct gctcgcgcgc aaacgccgcg
     3001 cacgccgcgg cccaagcgca caccacggcc gcgctcgagc cgaggccgag cttctccgag
     3061 cctgcgaaca atgcgcgcga atcgatcgtc gcgtgcc
//
LOCUS       DATLEE010000228         2626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3981201, whole genome shotgun sequence.
ACCESSION   DATLEE010000228 DATLEE010000000
VERSION     DATLEE010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3981201"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="VM692_04835"
     CDS             complement(<1..1309)
                     /locus_tag="VM692_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HUQ51524.1"
                     /translation="MRSVTTNQPRHRKPREWVLAWLACGSLGTTAGLAQTDVTATDPV
                     LGGNPTATPSADTASDLVRADADTVASSQRLVDSNIAEFGRKSLQVGEAYFDLAEAQR
                     RAKQYEKAAESYLAAVEIYRSIDGPFSPLAIAPLTSLGDSYREADDNVNAVASYSEAR
                     TVSRRAYGLHNEEQIDLLDRMSRSLLDLNQLTEAEAQQVEALRLVQRSHPPDSDEVLE
                     AIYKYAAWLGDRLLFQLQRDQYTRALRIIRESHGEHDVRLVRPLLGIGNTYREERNPA
                     GPGISALQDALALLMEQPQHDPVAIAGALRDLGDWAVAFGKTGYDGKEYQRAWETLGA
                     APNGEQLRREWFSGANYVLYEPISPRGLSTEPDALSGHVTVSFDIDAMGNSSNVALVE
                     SDPPGLKDEAVLRHIRRSRFRPLVSEGHIVTGRNLAIQVKFRYL"
     gene            1511..>2626
                     /locus_tag="VM692_04840"
     CDS             1511..>2626
                     /locus_tag="VM692_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011642290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUQ51525.1"
                     /translation="MDPTDTHGTEYFHRVVDCQWACPAHTDVPEYIRLVAQGRFGDAY
                     LLNRQSNVFPGILGRVCDRPCEPACRRGRVEEKPVAICRLKRVAADHRGEIEHRLPRP
                     AARKNGRRVALIGAGCASLTVANDLAPLGYDCVIFEALGTLGGLMRTNIPAFRLPGEV
                     LDEEIGYVTRLGVDVRLNSPVKSLRSLLDEGFDAVFVGSGAPKGKNLRLPGRDASDRI
                     HIGIDWLGSIAFGHIDSVGERVLIIGVGNTAMDCCRSSLRLGAKDVKVIARKPRQFFK
                     ASDWELEDAEEERVEIVVNHSPKEFVIEGGKLVGMRFDELAYELDAAGEITATKVVRE
                     VFLPCDDVVLAIGQENAFPWIERDLGLTFDKYDVPVVD"
BASE COUNT          430 a          824 c          860 g          512 t
ORIGIN      
        1 gcaaatatct gaatttgacc tggatcgcca ggttcctgcc ggtcacgatg tgcccttcgg
       61 agaccaacgg gcggaaacgc gagcggcgaa tgtgacgcag cacggcctcg tccttcaacc
      121 ccggcgggtc ggattcgacg agcgcgacgt tgctgctgtt ccccatggcg tcgatgtcga
      181 agctgacggt gacgtgaccg ctcaacgcat cgggctcggt gctcaagccg cgcgggctga
      241 tcggctcata gagcacgtag ttcgcgccgg agaaccactc gcgccggagc tgctcgccgt
      301 tcggcgccgc ccccagtgtt tcccacgcgc gttgatactc cttgccgtcg tagccggtct
      361 tgccgaacgc aacggcccaa tcgccgaggt cgcgcaacgc gcccgcaatc gcgacggggt
      421 cgtgttgcgg ctgctccatc aacagtgcca gggcgtcttg cagcgcggaa atcccgggcc
      481 ccgcaggatt gcgttcttcg cgatacgtat tgccgatgcc gagcagcggt ctgaccaggc
      541 gcacgtcatg ctcgccatga gactcgcgga tgatgcgcag agcccgtgtg tattgatcgc
      601 gctgtagctg aaacagcaag cggtcgccta gccaggcggc atatttatag atggcttcga
      661 ggacctcgtc ggaatccggc ggatggctgc gttgcacgag ccgcaacgcc tcgacctgct
      721 gggcttccgc ttcggtgagc tggttcaggt cgagcagcga gcgcgacatg cggtcgagca
      781 ggtcgatctg ctcctcgttg tgcagcccat aagcgcggcg actgacggtg cgcgcttcgg
      841 agtacgaggc caccgcgttg acgttgtcat cggcctctcg ataactgtcg cctaggctcg
      901 tgagcggtgc gatagcgagc ggagagaagg ggccgtcgat ggaacgatag atttcgacgg
      961 cggcgagata gctctccgcg gctttctcgt attgtttggc gcggcgttgt gcttcggcga
     1021 gatcgaagta cgcttcgccg acttgcaagc tcttgcggcc gaactcggcg atgttgctgt
     1081 cgacgaggcg ctgactcgag gcgaccgtgt cggcatcggc gcggacgaga tccgaggccg
     1141 tgtccgcgct cggtgtggcc gtcggatttc ctccgaggac gggatcggtg gcggtgacgt
     1201 ctgtttgcgc gagaccggcc gtggtcccca agctgccgca cgcgagccag gcgagcaccc
     1261 attctcgcgg cttgcgatgc cgaggttggt tggtcgtgac ggacctcatc gctggcattc
     1321 tatccccaat acaatgtcat gccgcagccg tggcacgaat cggagatgca cgccgcgcaa
     1381 aggctttagt cccgcccgaa ctcggtaacg ttcagcgtgt gccggtgtcc gcatatgtct
     1441 ataatggcgc ccgtttcgcc gccccgcccg aattcaatgg tgctcctcac ggtctagctg
     1501 ctgccgcctg atggatccta ccgataccca cggcaccgag tacttccacc gcgttgtcga
     1561 ttgccaatgg gcatgcccgg cgcacaccga cgttccggaa tacattcggc tcgtcgcgca
     1621 ggggcggttc ggcgacgctt atttgcttaa ccgccagtcg aacgtctttc cgggaatcct
     1681 cggtcgagtt tgcgatcggc cctgtgagcc ggcctgccgc cgcggtcgcg tcgaagagaa
     1741 gccggtcgcg atctgccgct tgaagcgagt ggccgccgac catcgcggcg agatagagca
     1801 tcgtctgcct cgaccggctg cgcgtaagaa cggtcgacgc gtcgctctga tcggtgccgg
     1861 ctgcgcgtct ctgacggtcg cgaacgacct cgcgccgctc ggctacgact gcgtgatctt
     1921 cgaagccctc ggcaccttgg gcggcctcat gcgcaccaat attccggcgt ttcgcttgcc
     1981 gggcgaggtg ctcgacgaag agatcggcta cgtgactcgg ctcggcgtag acgtgcgctt
     2041 gaactcgccc gtgaagagcc tgcggtcgct actcgacgag ggtttcgacg ccgtgttcgt
     2101 cggcagcggc gcgccaaagg gcaagaattt gcgcctgccc ggccgcgacg ccagcgatcg
     2161 catccacatc ggcatcgatt ggctcgggtc gatcgcgttc ggccatatcg actccgtcgg
     2221 cgagcgcgtg ttgatcatcg gcgtcggcaa cacggccatg gattgttgcc gcagctcgtt
     2281 gcggctcggc gccaaggacg tcaaggtcat cgcgcgcaag ccgcgccagt tcttcaaggc
     2341 gtccgactgg gagcttgagg acgcggaaga ggagcgcgtc gagatcgtcg tcaatcactc
     2401 gccgaaggaa ttcgtgatcg aaggcggcaa gctcgtcggc atgcgcttcg acgagctcgc
     2461 ttacgagctc gacgccgccg gcgagatcac ggcgacgaaa gtcgtgcgcg aggtcttttt
     2521 gccgtgcgac gacgtcgtgc tcgcgatcgg ccaggaaaac gcctttcctt ggatcgagcg
     2581 cgatctcggc cttacgttcg acaagtacga cgttcccgtc gtcgat
//
LOCUS       DATLEE010000229         2555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5480256, whole genome shotgun sequence.
ACCESSION   DATLEE010000229 DATLEE010000000
VERSION     DATLEE010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5480256"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VM692_04845"
     CDS             <1..332
                     /locus_tag="VM692_04845"
                     /inference="COORDINATES: protein motif:HMM:NF022060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate oxidase C-terminal
                     domain-containing protein"
                     /protein_id="HUQ51526.1"
                     /translation="APAAESDRYFASRPVASQINAWSSEQSRPLADAAALEQRAAQVT
                     ASKPAGEPLPRPEFWGGFRLWLEAVELWVEGQNRFHERVRYERRLDARDDYSFAPARW
                     TRQRLQP"
     gene            352..540
                     /locus_tag="VM692_04850"
     CDS             352..540
                     /locus_tag="VM692_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HUQ51527.1"
                     /translation="MIQFETITCPYCWEAIEISVDLSIDSQQQVEDCSVCCRPIVITY
                     SAAEGELVALDVAAENRE"
     gene            537..>2555
                     /locus_tag="VM692_04855"
     CDS             537..>2555
                     /locus_tag="VM692_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51528.1"
                     /translation="MTRCLAALLLGLAAAAAIAEERIADIRQGTNLSVALAPNGGALV
                     VDLVGQLWSLPATGGGAVPLTPADEQARNPRFSPDGTRLVYQRRGAADWDLWILELAT
                     GEQRPLSTSSFDERDPDFTRDGRAVVFATNRTGHYCLWSITLDGGVETQLTEEDGAAS
                     YPSVSEHGEVAYLLTRGDESSLRVLGGDGAISVLHTSSGRLSAPTWRPGGGALIFGEQ
                     DSAETSRLQLLLPGEPRVLKALSGSEDLFASRAAWRSGAEFIYAADGQLWRRGIATPT
                     RDPVHLFAAAAVEVAAQPTDVPAFDAAGERVALGVNGVAKSPDGRRTVFTALGDLWLA
                     DRGEPRRLTDDAYVDLDPGFWPDGESVVFASERTGQFELWRLTLRDGKVTQLTFGALE
                     PRHPAVRPDGNTIAYFESASLEPAAATSLKSLDLRRRDETTVATGVVAGTELAWSEDG
                     RALVVHARSAIVANPRDFRVDIRTEPTPVAANRPEEPLPSARWQVPAAPSDYVVEIGR
                     LFDGIRGAYQRHVDLHVRGGRIAAIVPRGTLPASGEIIDARDATVIPGLIDLHAHQSS
                     LVGERLGRAWLAYGVTTVRELSAAPGEAVERAEAWASGRSAGPRLLVSAAGSAPPAAR
                     AALRPYPGIAHGFAHSLQRQAREIGVPALAASSFPPRLHSDFDEPSAEL"
BASE COUNT          367 a          913 c          898 g          377 t
ORIGIN      
        1 gagcgccggc tgccgagagc gaccggtatt tcgcgagccg ccctgtcgcg agccagatca
       61 acgcctggag cagcgagcaa agccggccgc tcgcagacgc ggccgcgctc gaacagcgcg
      121 cagcgcaggt gacggcatcg aagcccgccg gcgagccgct tcctcgcccg gaattctggg
      181 gcggcttccg gctgtggctg gaagccgtcg agctctgggt cgaagggcag aaccgcttcc
      241 acgagcgcgt gcgctacgag cgccgcctcg acgcgcgcga cgactacagc ttcgcgccgg
      301 cgcgctggac ccgccagcgc ctgcaaccgt agcgcgaccg cgagccctcg cttgatccaa
      361 ttcgaaacga tcacgtgtcc gtattgctgg gaagcgatcg agatcagcgt cgatctctcc
      421 atcgactcgc agcagcaagt cgaggattgc tctgtctgct gccggccaat cgtgatcacc
      481 tatagcgccg cagaaggcga gctcgtcgcg ctcgacgttg cggccgagaa tcgcgagtga
      541 cgcgctgtct cgccgcgttg ctgctcggcc tcgccgctgc cgcggccatc gctgaagagc
      601 gcattgccga catccggcaa ggaacgaacc tgtccgtcgc gctcgcgcct aacggcggcg
      661 cgctcgtcgt cgatctcgtc ggccagctct ggagcttgcc cgcgacgggc ggcggagccg
      721 tgccgttgac accggcggac gagcaggcgc gcaacccgcg gttcagcccc gacggtacgc
      781 ggctcgtcta ccaacgtcgc ggcgccgcgg actgggacct gtggatactc gagctcgcaa
      841 cgggcgagca acggccgctc agcacgtcgt cgttcgacga gcgtgacccg gatttcactc
      901 gcgacggccg cgcagtggtc ttcgcaacga atcgcaccgg tcactactgc ttgtggtcga
      961 tcacgctcga cggcggcgtc gagacgcagc tgaccgagga agacggcgcg gcttcgtatc
     1021 cgagcgtctc ggagcacggc gaagtggctt atctcctgac gcgtggcgac gagtcgtcgc
     1081 tgcgtgtgct cggcggtgac ggcgcgatca gcgtgctgca cacgagctcg ggccgcttat
     1141 cggcgccgac gtggcggccc ggcggcggcg cgctgatttt cggcgagcag gactcggccg
     1201 agacgagccg tctgcagttg ctgctgcccg gcgagccgcg cgtgctgaag gcgctctcgg
     1261 gcagcgaaga tttgttcgcg tcgcgcgcgg catggcgctc gggcgccgag ttcatctatg
     1321 cggccgacgg tcagctctgg cgccgcggca tcgcgacgcc gacgcgcgac cccgtgcact
     1381 tgttcgccgc ggccgccgtg gaagtcgctg cgcagccgac cgacgtgccg gccttcgatg
     1441 cggccggcga gcgcgttgcc ctcggcgtca acggcgtcgc aaagtcgccg gacgggaggc
     1501 ggacggtatt cacggcgctc ggcgacctct ggctggccga ccgcggcgag ccgcgccgcc
     1561 tgaccgacga cgcgtacgtc gacctcgatc ccggcttttg gcccgatggc gaatccgtcg
     1621 tgttcgcgag cgagcgcacg gggcagttcg agctgtggcg gctgacgttg cgcgacggca
     1681 aggtcacgca gctgacgttc ggcgcgctcg agcctcggca cccggccgtg cggcccgacg
     1741 gcaacacgat cgcgtatttc gaaagcgcca gcctcgagcc cgcggccgct acgtccttga
     1801 agtcactcga cctgcgccgc cgcgacgaga cgaccgtggc gaccggcgtc gtggccggca
     1861 cggagctcgc ctggtcggag gacggccgag cactcgtcgt ccacgcccgc tctgccatcg
     1921 tcgcgaaccc gcgcgacttc cgcgtcgaca tccgcaccga gccgacgccg gtcgccgcga
     1981 accggcccga agagccgctg ccgtcggctc gctggcaagt gccggccgcg ccgagcgact
     2041 acgtcgtcga gatcggccgg ctgttcgacg gaatccgagg cgcctatcag cggcatgtcg
     2101 accttcacgt gcgcggcggc cgcatcgccg cgatcgtgcc gcggggaacg ctgccggcga
     2161 gcggcgagat catcgacgcg cgcgatgcca ccgtgatccc cggcctcatc gacctgcacg
     2221 ctcatcagag ctcgctcgtc ggcgagcggt tgggccgcgc atggctcgcc tatggcgtca
     2281 cgacggttcg cgagctcagc gcggcgcccg gtgaggccgt cgaacgcgcc gaggcttggg
     2341 caagcgggcg ttcggcgggc ccgcgtctgc tcgtgtccgc agccggcagt gcgccgcccg
     2401 ccgcgcgggc cgcgctgcgg ccgtaccccg gtatcgcgca cggcttcgcg cacagtctgc
     2461 agcgacaagc gcgcgaaatc ggcgtaccgg ctttggcagc gagctccttt ccgccgaggc
     2521 tgcacagcga cttcgacgaa ccgagcgccg agctc
//
LOCUS       DATLEE010000230         2675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9201113, whole genome shotgun sequence.
ACCESSION   DATLEE010000230 DATLEE010000000
VERSION     DATLEE010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9201113"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VM692_04860"
     CDS             complement(<1..298)
                     /locus_tag="VM692_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ51529.1"
                     /translation="MRQLTLLGSLLLCCVAANAQSLLATLIQNGDRAAALEALHAPDA
                     DVNATQGDGTTPLHWAVYKVDRELVTELLDHGAKADVTNRYGSSPLAEAAKLGDL"
     gene            complement(295..1200)
                     /locus_tag="VM692_04865"
     CDS             complement(295..1200)
                     /locus_tag="VM692_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ51530.1"
                     /translation="MRVNRRTVLQGALGAAVAASIRPVFAKAAAITDELTLLGGFGGN
                     VVALKTSDGAVLVDSGVAMPNSPLLALTREATGTEHVHTLFNTHWHHEQIGGNEALGT
                     AGAHIVAHEKTRLRLATPYFLPERDEYQPAMPVKAQPTESFYTTGSTTIGGRRIEYGY
                     LPEAHTDGDAYVYFRDDNVIAVGDALAPERDVELDWYGGGWLGGRVDSLKKLLELTDA
                     QTKFVPSYGAPVGRAAVQAEHDMCNLLFERFFERVRKGETPEDMLAAGIMKDTGRTWS
                     DPAKFVHDTSKGFWGHNNTLSHDIV"
     gene            complement(1200..2546)
                     /locus_tag="VM692_04870"
     CDS             complement(1200..2546)
                     /locus_tag="VM692_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ51531.1"
                     /translation="MFLTKKHLSRRTVLKGAGVSLALPLLDAMVPAATALAQTAAAPK
                     LRVGFFYIPHGAIQWNTSHGPAMDRWNPSGSGASFKLSEILSPLEEFKHLVASFSNLE
                     NKANENSVHAIVPSTWLSGTRPDTRAQGANMATTIDQLIAAHIGQETALPSLEVASET
                     TAQAAACPSGAGCYYSTTLSFRNPTSPLPMEFNPRKVFTQLLGEGDTDAERAAVTKQT
                     RSLLDLIADRTAALQKQVGAGDRRLLDEYLSTVREIERRVQMAEQRDLSAITLPEAPI
                     GELESFDEQVKLMFDLIALAYQANLTRVASYIMVAEGTQRTYNHIGVSEAFHPLSHHA
                     NKMDSLEKLVKIQKYHVERFAGFVKKLAATQDGDGSLLDHSLFMYGSNMSNSDKHNNY
                     PLPNILVGGANGNLQGGQQVELPEHTALTNLLLTVLNKAGLEMKSFSDSTGAIAGV"
BASE COUNT          405 a          856 c          902 g          512 t
ORIGIN      
        1 cgaggtcgcc gagcttcgcg gcctcggcga gcgggctcga gccgtacctg ttcgtgacgt
       61 cggctttggc gccgtggtcc agcagctccg tgacgagctc gcgatcgact ttgtagacgg
      121 cccagtgcag cggcgtggtg ccgtcgcctt gagttgcgtt gacgtcggcg tccggcgcgt
      181 ggagcgcttc gagcgcggcc gcgcggtcac cattctgaat cagcgtcgcg agcaagctct
      241 gtgcattcgc cgcaacgcag cagagcagca aggagccgag gagcgtcagc tgtctcatac
      301 gatatcgtgc gacaaagtgt tgttgtggcc ccagaaaccc ttgctcgtgt cgtgcacgaa
      361 cttggccggg tcgctccagg tgcggcccgt gtccttcatg atgccggccg cgagcatatc
      421 ctcgggtgtc tcgcccttgc ggacgcgctc gaagaagcgc tcgaacagca ggttgcacat
      481 gtcgtgctcg gcctgcacgg cggcgcggcc gacgggtgcg ccgtagctcg gcacgaactt
      541 cgtctgcgca tccgtgagct cgagcaactt cttcagcgag tcgacgcggc cgcccagcca
      601 gccgccgcca taccagtcga gctcgacgtc acgctcgggc gccaaggcgt cgccgacggc
      661 gatgacgttg tcgtcgcgga agtagacgta cgcgtcgccg tccgtgtgcg cttcgggcaa
      721 atagccgtac tcgatgcggc ggccgccgat cgtcgtcgag cccgtcgtat aaaagctctc
      781 ggtcggctga gctttgacgg gcatcgccgg ctggtactcg tcgcgctcgg gcaagaagta
      841 cggcgtcgcg agccgcagcc gagtcttttc gtgcgcgacg atgtgagcgc ctgcagtgcc
      901 gagcgcttca ttgcccccga tttgctcgtg gtgccagtgt gtattgaaca gcgtatgcac
      961 gtgctcggtg ccggtggctt cgcgcgtcag cgcgagcagc gggctgttcg gcatcgcgac
     1021 gccgctgtcg acgagcacgg cgccgtcgct cgttttcagc gcgacgacgt tgccgccgaa
     1081 gccgccgagc agggtcaatt cgtcggtgat cgccgcggct ttcgcgaaga cgggcctgat
     1141 cgacgcggcg accgccgcgc cgagcgcgcc ctgcagcacc gtgcggcgat tgacgcgcat
     1201 cagacgcctg cgatcgcgcc ggtgctgtcg gagaagcttt tcatctcgag gccggccttg
     1261 ttcaagaccg tcagcagcaa gttcgtcagc gccgtgtgct cggggagctc gacttgctgg
     1321 ccgccctgca agttgccgtt cgcgccgccg accaagatgt tcggcaacgg ataattgttg
     1381 tgcttgtcgc tgttgctcat gttcgagcca tacatgaaca acgagtgatc gagcaacgag
     1441 ccgtcgccgt cttgcgtcgc cgcaagcttt ttgacgaaac ccgcgaatcg ctcgacgtgg
     1501 tacttctgga tcttcacgag cttctcgagg ctgtccatct tgttcgcgtg gtgcgacagc
     1561 gggtggaacg cctccgagac gccgatgtgg ttgtaggtgc gctgcgtgcc ctcggcgacc
     1621 atgatgtacg acgcgacgcg cgtgaggttc gcctgatagg cgagggcgat caggtcgaac
     1681 atcagcttga cctgctcgtc gaacgattcg agctcgccga tcggcgcttc gggcagcgtg
     1741 atcgccgaca gatcgcgctg ctcggccatc tgcacgcggc gctcgatttc gcgcacggtg
     1801 ctcaagtact cgtcgagcaa ccgccggtcg ccggcgccga cttgcttctg cagcgcggcc
     1861 gtacggtctg cgatcaagtc gagcaagcta cgcgtttgtt tcgtgacggc cgctcgctcc
     1921 gcgtcggtgt cgccttcgcc gagcagctgc gtgaacactt tgcgcggatt gaactccatc
     1981 ggcagcggcg aggtcgggtt gcggaacgac agcgtggtcg agtagtagca gccggcgccg
     2041 gacgggcacg cggcggcttg cgccgtcgtc tccgatgcga cctcgagcga cggcagcgcc
     2101 gtctcttggc cgatgtgcgc cgcgatcagc tgatcgatcg tcgtcgccat gttcgcgccc
     2161 tgcgcgcgag tgtcgggccg cgtgcccgag agccacgtcg acggcacgat cgcgtgcacg
     2221 gagttttcgt tggctttgtt ctcgaggttc gagaacgacg caacgaggtg cttgaactct
     2281 tcgagcggcg acaggatctc gctcaacttg aagctcgccc cggagccgct ggggttccag
     2341 cggtccatcg ctgggccatg cgacgtgttc cactgaatcg cgccgtgcgg aatatagaag
     2401 aagccgacgc gcagcttcgg cgccgcggcc gtttgcgcga gcgccgtcgc cgcggggacc
     2461 atcgcgtcga gcagcggcag ggcgagcgat acgccggcgc ctttgagcac cgtgcggcgc
     2521 gacagatgct tcttcgtcaa aaacatggct actcccctgc gacccgcgcc gtcgtttcgt
     2581 tggcaggctc agcatcggcc ggcgcctgta ggcggaacga atcgctgttg acgatgccgg
     2641 ccacgatggc cccaaaccga tagttgtcgc gtgcc
//
LOCUS       DATLEE010000231         3525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5483598, whole genome shotgun sequence.
ACCESSION   DATLEE010000231 DATLEE010000000
VERSION     DATLEE010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5483598"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VM692_04875"
     CDS             <1..361
                     /locus_tag="VM692_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51532.1"
                     /translation="AVSILVQLLDIWFVWALLRTFNKQRRFLQTMTALLAAETLFTLA
                     GAPLAPLVPVSTSNEQQLTLPLFLIFLLGVWSIDVAAFVFSRAIERPYLLCVAIMLGY
                     VLLIFSLQASLLPLEVA"
     gene            361..1734
                     /gene="mpl"
                     /locus_tag="VM692_04880"
     CDS             361..1734
                     /gene="mpl"
                     /locus_tag="VM692_04880"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HUQ51533.1"
                     /translation="MRVHILGVCGTFMGGIAAIAKEAGHDVSGADQNVYPPMSTQLER
                     LGIKLVEGYEAPIDAGVQSVVVGNALSRGKPVVEALLNSGRPYSSGPQWLAEHVLHDK
                     WVLAVSGTHGKTTTTSMLAWILEYAGLQPGFLVGGVPSNFGISARLGGSKYFVVEADE
                     YDTAFFDKRAKFVHYRPRTLIVNNVEFDHADIYSDIDAIIWQFHQLLRTVPGNGLIVA
                     NGKDQNVERVLKRGVWTPLTTFSAHDRSAHWFASYDSVGAESTFSVFEQGAARGQGRW
                     TLLGQHNLENALAAMAAAGHVGVPLDVALAALAQFKGVKRRLELRGTFGGVSLYEDFA
                     HHPTAIATTLAGLKSRAPGQRIVAVMEPRSNTMRMGVHRDTLHGSFADADRVFVLGAA
                     DLGFDPGAALAPLGKRLTVETDVEALLARLLGELAAGDHVVLMSNGSFQGLPRLLEQA
                     LQNRTAS"
     gene            1760..2341
                     /locus_tag="VM692_04885"
     CDS             1760..2341
                     /locus_tag="VM692_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HUQ51534.1"
                     /translation="MSELPLFPLQTVLLPEGRLALRVFEPRYLDMVARTLRGENRFGV
                     IAIRHGSEVGGAATTYDVGTSAEIVDWHQETGGLLGILAVGREPFRLRATRREPDGLN
                     VGDVEWLPALAPQALPAKHEPLAALLRKLLEPLPQYRGVELALDDAVWVGGRLIELLP
                     LGLAFKQSLLEAADAVQRLDGLAAALRPSGRSE"
     gene            2396..2560
                     /locus_tag="VM692_04890"
     CDS             2396..2560
                     /locus_tag="VM692_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HUQ51535.1"
                     /translation="MKKYMCLICGFVYDEAEGRPDEGIPAGTKWADIPLSWRCPDCGS
                     GKEDFEMVEV"
     gene            complement(2576..3466)
                     /locus_tag="VM692_04895"
     CDS             complement(2576..3466)
                     /locus_tag="VM692_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HUQ51536.1"
                     /translation="MGHRSRARVSGKAWLAALALLLPAVAAADPAAWRMTGANGAEVT
                     LLGSMHVLRPSDHPLPVSVDALIDRAEIIVREIDMDDVDAAAQQRLILETAMLPQGTV
                     LANVIDEKVYEQLEQNAAQLGVDLKLLERFEPWFLSVTLLDQGLRKFGFQAERGVEQY
                     VLGRARRTNKQIVGLETLEFQLNIFDSLSRQSQQAMLEQTLAEIDEAEPALRAMADAW
                     RDGELETLSVELLDDFDGFPGLYETLVADRNRTWVPALERMLTEGRRYLVVVGALHLV
                     GDGSVIELLQARGHHVERLH"
BASE COUNT          552 a         1164 c         1189 g          620 t
ORIGIN      
        1 ggcagtctcg atcctcgtgc agttgctcga catttggttc gtctgggcgt tgctgcgcac
       61 gttcaacaag cagcgccggt tcctgcaaac gatgacggct ttgcttgccg ccgagacgct
      121 gttcacgctg gcgggcgccc cgttggcgcc gctcgtcccc gtgtcgacct ctaacgagca
      181 gcaactgacg ctgccgctgt tcctgatctt cctgctcggc gtgtggtcga tcgacgtcgc
      241 ggcgttcgtg ttctcgcgcg ccatcgagcg gccgtatctc ttgtgcgtgg cgatcatgct
      301 cggctatgtg ctgctgatct tcagtctgca agcttcgctg ctgccgctcg aggtggcgtg
      361 atgcgagtgc atatcctcgg cgtttgcggc acgttcatgg gcggcatcgc ggcaatcgcc
      421 aaggaggccg gccacgatgt gtcgggcgcc gaccagaacg tttatccgcc gatgagcacg
      481 cagctcgagc gcctcgggat caagctcgtc gagggctacg aggcgccgat cgacgccggc
      541 gtccagagcg tcgtcgtcgg caatgcgttg tcgcgcggca agcccgtcgt cgaggcgctg
      601 ctgaactccg gccggccgta ttcctcgggg ccgcaatggc tcgccgagca cgtgctgcac
      661 gacaagtggg tgctcgctgt ctcgggcacg cacggcaaga cgacgacgac gagcatgctc
      721 gcctggatac tcgagtacgc cggcttgcag ccgggcttct tggtcggcgg tgtgccaagc
      781 aacttcggca tctcggcccg gctcggcggc tcgaagtatt tcgtcgtcga agccgacgag
      841 tacgacacgg cgtttttcga caagcgagcg aagttcgtgc actaccgccc gcgcacgctg
      901 atcgtcaaca acgtcgagtt cgaccacgcc gacatctatt ccgacatcga cgcgatcatc
      961 tggcagtttc atcagctgct gcgcacggtg cccggcaacg ggctcatcgt cgcgaacggc
     1021 aaggaccaga acgtcgagcg cgtgttgaaa cgtggcgtct ggactccgct gacgacgttc
     1081 agcgcgcacg accgaagtgc gcactggttt gccagctacg attcggtcgg cgcggagtct
     1141 acgttcagtg tgttcgagca gggtgccgcg cgcgggcagg ggcgttggac gctgctcggc
     1201 caacacaacc tggagaacgc cctcgccgcg atggccgcgg ccggccacgt cggcgtcccg
     1261 ctcgacgtgg cgctcgcggc gctcgcgcag ttcaagggcg tgaagcgccg cctcgagctg
     1321 cgcggcacgt tcggcggcgt cagcttgtac gaggacttcg cgcatcaccc gacggccatc
     1381 gccacgacgc tcgccgggct caagagccgc gcgccggggc agcgcatcgt cgccgtcatg
     1441 gagccgcgct cgaacaccat gcgcatgggc gtgcatcgcg acacgctgca tggctcgttc
     1501 gccgatgccg accgcgtgtt cgtgctcggc gccgcggacc tcggcttcga tcccggcgcc
     1561 gcgctggcgc cgctcggcaa gcggttgacc gtcgagaccg acgtggaggc gctgctggcg
     1621 cgactgctcg gcgagctcgc ggccggagat cacgtcgtgc tgatgtcgaa cggcagcttt
     1681 caaggcttgc cgcggctgct cgagcaagcg ctgcaaaacc gcacggcgag ctgagccgac
     1741 gcgcgccata ggtggttcca tgagcgagct gccgctgttc ccgctgcaaa cggtcctgct
     1801 gccggaaggc cggctcgcgc tgcgcgtgtt cgagccgcgc tatctcgaca tggtcgcgcg
     1861 cactctgcgc ggcgagaatc gcttcggcgt gatcgcgatt cggcacggct ccgaggtcgg
     1921 tggcgccgcg acgacctacg acgtcggcac gagcgccgag atcgtcgact ggcatcaaga
     1981 gacgggcggc ctgctcggga ttctcgccgt cgggcgcgag ccgtttcgcc tgcgcgcgac
     2041 gaggcgcgag cccgacggtc tcaacgtcgg cgacgtcgag tggctgccgg cactggcgcc
     2101 gcaggcgctg ccggcaaagc acgagccgct cgcggcgctg ctgcgcaagc tcctcgagcc
     2161 gctaccgcaa tatcgcggag tcgagctcgc gctcgacgat gccgtctggg tcggcggccg
     2221 gctgatcgag ctgttgccgc tcgggctcgc gttcaagcaa tcgctgctcg aggcggccga
     2281 tgccgtgcag cggctcgacg ggttggctgc ggcgctgcga ccgagcggcc gaagcgagtg
     2341 acgcagcggc tcatggtagg cttgtgaaaa agcgccccga gctcaagtag gtgtcgtgaa
     2401 aaagtatatg tgtctcatct gcggcttcgt gtacgacgaa gccgagggtc ggcccgacga
     2461 aggaattcct gcgggcacca agtgggccga cattccgctg tcgtggcgct gccccgattg
     2521 cggctccggc aaggaagact tcgaaatggt cgaggtgtga tcggccgaga atgcgtcaat
     2581 gcaaacgctc gacgtgatgc ccgcgcgcct gcaatagctc gatgacactg ccgtcgccta
     2641 ctaggtgcaa ggcgcccacg acgacgagat aacgccgccc ttccgtcaac atgcgctcga
     2701 gagcgggaac ccacgttcga ttgcggtccg ccacgagcgt ctcgtagagc cctgggaagc
     2761 cgtcgaaatc gtcgagcagc tcgacgctca aggtctcgag ctcgccgtca cgccaagcgt
     2821 cggccatcgc gcgcagcgcg ggctcggctt cgtcgatctc cgccaacgtt tgctcgagca
     2881 tcgcctgctg cgactgccgc gataacgagt cgaagatgtt gagctgaaac tcgagcgtct
     2941 cgaggccgac gatttgtttg ttcgttcgtc gtgctcggcc gagcacgtac tgctcgacgc
     3001 cgcgctcggc ctgaaagccg aacttacgca ggccctgatc gagcagcgtg acggacagga
     3061 accacggctc gaaccgctcg agcagcttca gatcgacgcc gagctgcgcg gcgttctgct
     3121 cgagctgctc atagaccttc tcgtcgatga cgttcgcgag cacggtgccc tgcggcagca
     3181 tcgccgtctc gaggatcagg cgctgctgcg cggccgcgtc gacgtcgtcc atgtcgatct
     3241 ccctgacgat gatctcggcg cggtcgatga gggcgtccac ggaaaccggc agcggatgat
     3301 cgcttggccg caagacgtgc atcgatccga gcagcgtaac ctcggcgccg ttggcgcccg
     3361 tcattcgcca cgcggccgga tcggctgctg caacggccgg tagcagcagt gcgagggccg
     3421 cgagccaagc cttgccgctc acgcgcgcgc ggctccggtg acccatgcta cctcggtttc
     3481 gatacggccg tcgtcgtaga gctcgatcca gcgcatgccc ggcgg
//
LOCUS       DATLEE010000232         2875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1012357, whole genome shotgun sequence.
ACCESSION   DATLEE010000232 DATLEE010000000
VERSION     DATLEE010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012357"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VM692_04900"
     CDS             complement(<1..207)
                     /locus_tag="VM692_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51537.1"
                     /translation="MTYFSQSRLMLESEQAINEHAHAGTPIIQRVCTQLLNEPTRYVR
                     WHVRHEQRMGAIAEARVRQRQILAL"
     gene            complement(355..747)
                     /locus_tag="VM692_04905"
     CDS             complement(355..747)
                     /locus_tag="VM692_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUQ51538.1"
                     /translation="MTDKTVTIAAGMLLTLCAATSLAQPPQGSGPRGKEVYDYWCLPC
                     HGPGLGLPGFNELPGTQQLRIKYRDTQVSPLLEERTDLVPVFIKTIVRQGVSFMPQFR
                     KTEISDADLDALAAYLARNSPAGPQPPR"
     gene            complement(744..2378)
                     /locus_tag="VM692_04910"
     CDS             complement(744..2378)
                     /locus_tag="VM692_04910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUQ51539.1"
                     /translation="MPAPPPGVSAADFTAALRKLEAAVGEQWVFKTDEDVALYRDAYS
                     PERNEPTERLASAAVAPIDVEQVQQVVRIANEHKLPLYPISTGKNLGYGGSAPILSGS
                     VVVDLKRMNRIIAVDEGRCSAVVEPGVSYFDLYRHIQERKLKLWIDTPDPGWGSVVGN
                     ALDHGVGYTFGQYRDHFGSHCGMEVVLPNGELMRTGMAALAKDASWADYKYGFGPHVA
                     ALFGQANFGIVTKMGFWLMPQPEAYRRGTVLVPKRRDIVPLVDHVNHLENLGLIGMPG
                     FRSPLGAAQAREPELRALVAKQGGPTDEELDRFAESKGLASWSVELQFYGPEKTIAAN
                     WDYVRERVGAAIPGSRFEDGVSYRFPLTPEQQAEVQRVHRLVDIGIPNMSIFAIAGGR
                     TPQNPDSADGGQFYFAPMIPRTGEAILEANRVLGQAYREAGVPYNQYFAAPACWHYRA
                     FIMITSLPVSRLDPEINRRSRAAFERLARVAAEHGWGEYRSPPFGVDLVASAYSFNDH
                     ALRRFCETLKDAVDPNGIIAAGRGGIWPKHLRGTRA"
     gene            complement(2391..>2875)
                     /locus_tag="VM692_04915"
     CDS             complement(2391..>2875)
                     /locus_tag="VM692_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51540.1"
                     /translation="DAGDITRFWYDELDSLWQREPVAIAGLTQFGPMFVVERLALERR
                     MQVVLRVEHCSGSDGTLRHSLTGPGETLALAARFDTLHADWPGLTAALACSVAADDSP
                     RDNAAFTTTHSPPGSGALAGSAAPSVIHYYTPLAVQQGYGPALDGPLYSWVVAPRARA
                     "
BASE COUNT          462 a          943 c         1005 g          465 t
ORIGIN      
        1 caacgccaag atctgtcttt ggcgcacccg tgcctctgca atggcgccca tgcgctgctc
       61 gtgacgcacg tgccaacgca catagcgcgt cggctcgttc agcagctgcg tgcaaacgcg
      121 ttgaatgatg ggcgtgcccg catgggcatg ctcgttgatg gcctgctcgg actcgagcat
      181 gaggcgcgat tggctgaaat aagtcacgga tctcggcccg tttggcgtcc ggcgcccaag
      241 ttaggcgggc gacgtgacgt aggcgtgaca ctagcacctc gagcggggag gtgccatgca
      301 gttcttccac ttcggtggaa tacggcggcg cgcagtccgg ccgagcgtgg cctctcagcg
      361 cggcggctga gggcccgcag gactgttgcg cgcgagatac gccgcgaggg cgtcgaggtc
      421 ggcgtcgctg atctcggtct tgcgaaattg cggcatgaac gagacgccct ggcggacgat
      481 cgtcttgatg aacacgggca cgaggtccgt gcgctcctcg agcagcggcg agacctgcgt
      541 gtcgcggtac ttgatgcgca gctgctgcgt gccaggcagc tcgttgaacc ccggcagccc
      601 aagcccaggg ccgtggcacg gcaagcacca atagtcgtag acctctttgc cgcgaggccc
      661 gctgccctgc ggcggctgcg ccaggctggt agccgcgcac agcgtcagca gcatgccggc
      721 ggcgatcgtg accgtcttgt ccgtcatgcg cgagtgcctc gcaagtgctt cggccagatg
      781 ccgccccggc ccgcggcgat gatgccgtta ggatcgacgg cgtccttcaa ggtctcgcag
      841 aatctgcgca gcgcgtgatc gttgaacgag taggcgctcg ctacgagatc cacaccgaac
      901 ggcggcgacc gatactcgcc ccagccgtgc tcggccgcga cgcgcgcgag gcgctcgaat
      961 gccgcgcgcg accggcggtt gatctcggga tcgagccggc tcacgggcag gctcgtgatc
     1021 atgatgaacg cgcggtagtg ccagcacgcc ggtgccgcga aatactggtt gtagggcacg
     1081 ccggcctcgc gataggcctg gccgagcacg cgattcgctt cgaggatcgc ctcgccggtc
     1141 cgcgggatca tcggcgcgaa atagaactga ccgccgtcgg ccgagtccgg attctgcggc
     1201 gtgcgcccgc cggcgatcgc gaagatgctc atgttcggga tgccgatgtc gacgagtcgg
     1261 tgcacgcgct gcacctcggc ttgctgctcc ggagtcaacg ggaaccggta cgagacgccg
     1321 tcctcgaacc gcgagcccgg gatcgcggca ccaacccgct cgcgcacgta atcccaattg
     1381 gccgcgatgg ttttctcggg tccgtagaac tgcagctcga cgctccagga cgcgaggccc
     1441 ttcgactcgg cgaagcggtc gagctcctcg tccgtggggc cgccctgctt agcgaccagc
     1501 gcgcgcagct cgggctcgcg cgcctgcgcg gcgccgagcg ggctgcggaa acccggcatg
     1561 ccgatgaggc ccaagttctc gagatggttg acgtggtcga cgagcggcac gatgtcgcgc
     1621 cgcttcggca cgagcacggt gccgcgccgg tacgcttcag gctgcggcat gagccaaaag
     1681 cccatcttcg tgacgatgcc gaagttcgcc tggccaaaaa gcgccgcgac gtgcgggccg
     1741 aagccgtatt tgtaatcggc ccacgacgcg tccttcgcga gcgccgccat gccggtccgc
     1801 atgagctcgc cgttcggcag cacgacttcc atgccgcagt ggctgccgaa gtgatcgcga
     1861 tattggccga acgtgtagcc gacgccgtga tcgagcgcat tgccgacgac gctgccccag
     1921 ccgggatccg gagtgtcgat ccaaagtttc agcttgcgct cttggatgtg gcgatacagg
     1981 tcgaagtagc tgacaccggg ctcgacgacg gcgctgcacc ggccctcgtc gacggcgatg
     2041 atgcggttca tgcgcttcaa gtcgacgacg acgctgccgg acaggatcgg cgcggagccg
     2101 ccgtagccca ggttcttgcc ggtcgagatc ggatagagcg gcagcttgtg ctcgttcgcg
     2161 atgcgcacga cttgctgcac ttgctcgacg tcgatcggcg ccacggcggc cgacgcgagc
     2221 cgctccgtcg gctcgttgcg ctccggcgag tacgcgtcgc gatagagcgc gacgtcctcg
     2281 tcggtcttga agacccactg ttcgccgacg gcggcttcga gcttgcgcag cgccgcggtg
     2341 aaatcggcgg cgctgacacc tggaggcggg gctggcattt cgttacctcg tcaagctcgt
     2401 gcgcgcggcg cgacgaccca ggaatagagc ggcccgtcga gcgcgggccc gtagccctgc
     2461 tgcacggcga gcggcgtgta gtagtgaatg acggacggtg cggccgagcc ggccagcgca
     2521 ccggagcccg gcggcgagtg cgtcgtcgtg aaagcggcgt tgtcgcgcgg cgaatcgtcg
     2581 gcggcgacgc tgcacgcgag agcagccgtc aagcccggcc aatcggcgtg cagggtgtcg
     2641 aagcgggccg cgagcgccaa cgtctcgcct ggacccgtca gcgagtgacg cagcgtgccg
     2701 tcgctgccgc tgcaatgctc gacgcgtagc acgacctgca tgcggcgctc gagcgcgagc
     2761 cgctcgacga cgaacatcgg gccgaactgc gtgaggcccg caatcgcgac gggctcgcgt
     2821 tgccaaagcg agtcgagctc gtcgtaccaa aagcgcgtga tgtcgccggc gtcga
//
LOCUS       DATLEE010000233         6092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10568577, whole genome shotgun sequence.
ACCESSION   DATLEE010000233 DATLEE010000000
VERSION     DATLEE010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10568577"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VM692_04920"
     CDS             complement(<1..544)
                     /locus_tag="VM692_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HUQ51541.1"
                     /translation="MATPTYQVFAIKYAHVGRKAWGNFVDGDPHEDWDMPLDYYVWAI
                     VGDGRTFVVDTGFDAPRAKKRDRKIVHPVEEGLAAVGVRHADVKDVIVTHMHYDHCGN
                     LDLFPRARYHVQDKEMEFATGRCMCHGLMRHAFDADDVVTMVRKVFAGRVQFHDGIDE
                     LAPGLTIHHIGGHSKGLQCVR"
     gene            643..1521
                     /gene="yedA"
                     /locus_tag="VM692_04925"
     CDS             643..1521
                     /gene="yedA"
                     /locus_tag="VM692_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HUQ51542.1"
                     /translation="MSLSPLLVACLAATWIIWGSTYLAIKFALESFPPFYQMGSRFLV
                     AGLALMVWLKLRGRALPTLVEWRNAAIIGTLLLVSGMGFTAYSEQTVASGLVVAYIAI
                     MPIIVTVMNLPFGVRPSRLELVGMLVGFVGVLMLVRGQAFTASPTGLLAITIACVSWS
                     LGSVLSQRRFALAPGAMGFASEMLCGSVLLLLLSVIVREEPQWPPQPLAAWSWVYLIV
                     FGSLLAFNAYMVLLSRVSAALASSYTFVNPVIALALGIWLGGETVTSFEWVAAGIITA
                     GVVLLVLGRQPVKAAA"
     gene            complement(1526..2509)
                     /locus_tag="VM692_04930"
     CDS             complement(1526..2509)
                     /locus_tag="VM692_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51543.1"
                     /translation="MKGHWVAAAVAATLLRTAPAAAQSSGCDRACLEGLLDAYVDAVV
                     AHEPQRLPSTADVKFTENGQRLALGDGLWHTASGRGGYALKLADVERGQAVLMGTIRE
                     AEAPTVLVARLGVVNRRVAELETLVIRDAEVAERLDAIGAPRAAWSEPVPAAQRQSRA
                     ALVESANKYFSGIERNDGRGDYPLAADCARLENGLVLAGDVALVSPRAQGRPNGGQVG
                     CLEQFRSGIFFYVTRIRDRRFVLVDTERSVVFAFAFFDNAGGDSRFGTLPDGRRVESG
                     PKIPWTWAIAEVFKIERGLIGPVESVLQQVPYGMGSGWSTWQESLSSEPRD"
     gene            complement(2557..3528)
                     /locus_tag="VM692_04935"
     CDS             complement(2557..3528)
                     /locus_tag="VM692_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HUQ51544.1"
                     /translation="MRTTTLLASGIAALLTAGSALAQQPAQPAAPQPYRVGTPLSQAN
                     EAGQMKTMSSNVKVYGSFHFAESCTFDPDRNVIVAMNAGNPRDMGGPPPPLTPNDGFV
                     SLINPDGSVHTSKWIGVARDGLTLVHPLGSAIQGGTLYVNDGNYIRTFDLKTGTPKQS
                     FEVKEALNLNGIGVARDGTMYASNTGNPQRIYKVTASGQSSVFIDGAPLNQPNGVAID
                     PQGNIVVVNIGSNDVQTFDTSGKLVKTEHAKEAGNDGLVVLPDGTKYVSSVRFGSVSK
                     IAPGKEAEVIAEGIPSAASMCYDSKQNQLVIPMNPNNALAFIPLGRR"
     gene            3551..4017
                     /locus_tag="VM692_04940"
                     /pseudo
     CDS             3551..4017
                     /locus_tag="VM692_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
     gene            complement(4056..4484)
                     /locus_tag="VM692_04945"
     CDS             complement(4056..4484)
                     /locus_tag="VM692_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HUQ51545.1"
                     /translation="MAASKRKIKTVATATDPKKFIATIEGDAKRADSYELVEMMQEIT
                     GEPAKMWGSSIIGFGLYRYTYASGHSGEMCMTGFSPRSAALVLYLGPGIENETLMAKL
                     GKHKTGKGCLYVNKLDDVDRKVLRKLIEHSVKRLRKRGPS"
     gene            complement(4569..5063)
                     /locus_tag="VM692_04950"
     CDS             complement(4569..5063)
                     /locus_tag="VM692_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ51546.1"
                     /translation="MLQRTGTLALATMALAACQPAARGTDPNLARVLDRQAIEQLLAG
                     DYPHALDTRDFDAYAALFTDDGELSLQGNTSKGRAAIKQFVSALPAEPRVIHPITNLS
                     YEIDGDTATGGAYWQDVGLVNGLPGVLIAGRYEDTLRKVDGAWHIAKRSIVIEFAPPG
                     TPTQ"
     gene            5174..>6092
                     /locus_tag="VM692_04955"
     CDS             5174..>6092
                     /locus_tag="VM692_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HUQ51547.1"
                     /translation="MKQDPVIGFAPLSFPWQTKDPFLVCVHHVDLYPRGNDRLGPAAS
                     LEGRNIGQDFAGKDGWRMYHGDTVPGFPQHPHRGFETVTFVREGFIDHSDSLGATARF
                     GRGDVQWLTAGAGIVHSEMFPLLQPDGGNRLELFQIWVNLPAKSKFAAPYFTMFWDRD
                     VPRHRPAAGVELTIVAGAFADAQPPAPPPDSWAANSDADLAIWLITLDRGASWTLPSA
                     KSARTTRTLYFFSGPKLDVAGLTLGHHAAIEVVADQAVTLSAPDERAEVLLMQGRPIG
                     EPVAQYGPFVMNTRSELEQAFNDYRRTQFG"
BASE COUNT          923 a         1965 c         2076 g         1128 t
ORIGIN      
        1 cgcgcacgca ctgcagtccc ttcgagtgac cgccgatgtg atggatggtc aagccgggag
       61 ccagttcgtc gatgccatcg tggaactgca cccgtcccgc gaacaccttc cgcaccatgg
      121 tgacgacatc gtcggcgtcg aacgcgtgcc gcatgaggcc gtggcacatg cagcgccccg
      181 tggcgaactc catctctttg tcctgcacgt ggtagcgcgc ccgcggaaac agatcgaggt
      241 tgccgcagtg gtcgtagtgc atgtgcgtga cgatgacgtc cttgacgtcg gcatggcgca
      301 cgccgacggc cgcgaggcct tcctcgacgg ggtgcacgat cttgcggtcg cgcttcttcg
      361 cccttggggc gtcgaagccc gtgtcgacga cgaacgtgcg gccgtcgccg acgatggccc
      421 agacgtagta gtcgagcggc atgtcccaat cctcgtgcgg atcgccgtcg acgaagttgc
      481 cccacgcctt gcggccgacg tgagcgtatt tgatcgcgaa aacttgatag gtcggtgtgg
      541 ccatgggcgt ccgaggttgc cgagggtgtg attggcgcga attgtacttg gccggcgtag
      601 taagctcacg gcctcgtcga gggaggcctt aggagaccgc tcgtgtcgct atcgccgctg
      661 ttggtcgcgt gcctcgccgc cacctggatc atctggggct cgacgtacct cgcgatcaag
      721 ttcgcgctcg aaagctttcc gccgttctat cagatgggct cgcggttcct cgtcgcaggg
      781 ctcgcgctga tggtgtggct gaagctgcgc gggcgcgcgt tgccgacgct cgtcgaatgg
      841 cgcaacgcgg cgatcatcgg tacgttgctg ctcgtcagcg ggatgggctt cacggcgtac
      901 tccgagcaga ccgtggcttc ggggctcgtc gtcgcgtaca tcgcaatcat gccgatcatc
      961 gtgacggtca tgaacttgcc gttcggcgtg cggccgagcc gcctcgagct cgtgggcatg
     1021 ctcgtcggct tcgtcggcgt gctgatgctc gtgcgagggc aagcgttcac ggcatcgccg
     1081 accgggctcc tcgcgatcac gatcgcctgc gtgagctggt cgctcggcag cgtgctgtcg
     1141 cagcggcggt ttgcgctcgc gccgggcgcc atgggtttcg cgagcgagat gctgtgcggc
     1201 agcgtgttgc tgttgctgct ctcggtcatc gtccgcgaag agccgcagtg gccgccgcag
     1261 ccgctcgcag cttggtcgtg ggtctacctg atcgtgttcg gctcgctgct cgcgttcaac
     1321 gcgtacatgg tgctactgag ccgcgtcagc gcggcgctcg cgtcgagcta cacgttcgtg
     1381 aatcctgtca tcgcgctcgc gctcggcatc tggctcggcg gcgagacggt gacgtcgttc
     1441 gaatgggtcg ccgccggaat catcacggcc ggcgtagtcc tgctcgtcct cggcaggcag
     1501 cccgtcaagg cggccgcctg acgcgtcaat cgcgcggttc gctcgagagc gactcttgcc
     1561 acgtgctcca gccggagccc atgccgtacg gcacttgctg cagcaccgac tcgacggggc
     1621 cgatgagccc gcgctcgatc ttgaagactt cggctatagc ccaagtccac ggaatcttcg
     1681 ggccgctctc gacgcgtcgg ccgtcgggca gcgtgccgaa gcgcgagtcg ccgccggcgt
     1741 tgtcgaagaa ggcgaacgcg aacacgacgc tgcgctccgt atcgacgagc acgaagcgcc
     1801 ggtcgcggat gcgcgtcacg tagaaaaaga tgccgctacg aaactgctcg aggcagccga
     1861 cctgaccgcc gttcggccgg ccttgcgcgc gcgggctgac gagagcgacg tcgccggcga
     1921 gcacgagacc gttctcgagc cgcgcgcaat cggccgcgag cggatagtcg ccgcggccgt
     1981 cgttgcgctc gatgccgctg aaatacttgt tcgcgctctc gacgagcgcc gcgcgcgact
     2041 gccgctgcgc ggccggcacc ggctcggacc aagctgcccg cggcgcgccg atcgcgtcga
     2101 ggcgctctgc gacctcggcg tcgcggatca cgagcgtctc gagctcggcg actctgcgat
     2161 tcacgacgcc gaggcgcgcg acgagcacgg tgggcgcttc ggcttcgcga atcgtcccca
     2221 tgagcacagc ctggccccgc tcgacgtcgg cgagcttcag cgcgtaaccg ccgcggcccg
     2281 aggccgtgtg ccagaggccg tcgccgagcg cgagacgctg gccgttctcc gtgaacttga
     2341 cgtcggcggt cgacggtaag cgctgcggct cgtgcgcgac gacggcgtcg acgtacgcgt
     2401 cgagcaaccc ttcgaggcag gcacgatcgc agccgctcga ctgagcggct gcgggtgcgg
     2461 tgcggaggag ggttgcggcg acggccgccg caacccagtg gcctttcaag cgttaacggc
     2521 tgaggtcttg ccgcctaccg gcggcggttt taccgcctac cggcggccta gggggatgaa
     2581 cgccagcgcg ttgttcggat tcatcggaat cacgagctgg ttctgcttcg agtcgtagca
     2641 catcgacgca gccgacggga tgccttcggc gatgacctcg gcttccttgc ccggcgcgat
     2701 cttcgagacg ctgccgaagc gaacgctgct gacgtacttc gtgccgtcgg gcagcacgac
     2761 gaggccgtcg ttgcccgctt ccttggcgtg ctcggtcttg acgagcttgc cgctcgtgtc
     2821 gaacgtctgc acgtcgttgc tgccgatgtt gacgacgacg atgttgccct gcgggtcgat
     2881 cgcgacgccg ttcggctggt tcagcggtgc gccgtcgatg aacaccgacg attggccgct
     2941 cgccgtgacc ttgtagatgc gctgcgggtt gcccgtgttc gacgcgtaca tcgttccgtc
     3001 gcgcgcgact ccgatgccgt tcaagttcag cgcctctttg acttcgaacg actgtttcgg
     3061 cgtgccagtc ttcaggtcga acgtgcggat gtagttgccg tcgttgacgt agagcgtgcc
     3121 gccctggatg gcgctgccga gcggatgcac gagcgtgagg ccgtcacgcg cgacgccgat
     3181 ccacttcgac gtgtgcacgg agccgtccgg gttgatcagc gagacgaagc cgtcgttcgg
     3241 cgtcagcggc ggcggggggc cgcccatgtc ccgcggattg ccggcgttca tcgcgacgat
     3301 cacgttacgg tccggatcga acgtgcagct ctcggcaaaa tgaaagctgc cgtagacctt
     3361 cacgttgctc gacatcgttt tcatctggcc ggcttcgttc gcctggctca gcggcgtgcc
     3421 gacgcgatac ggttgcggag cggcgggctg agccggctgt tgcgcgagcg cgctgcccgc
     3481 tgtcaacaat gccgcgatac cggaagccaa tagagttgta gtgcgcacga gtccccccaa
     3541 gggttagtca atgtttcgaa gaggattttg ccctacggcg gtgcaacgcg tcgaattggt
     3601 aatcgtgaaa gccagcaagg aatccgaggc aggtcagatg ccgagcaccg agctcctgac
     3661 ggccatgggc aaattcaacg aagagctggc caaggcgggc tcatgctggc gggcgagggg
     3721 ctccacccaa gcgccaaggg caagcgcgtg cggttctcgg ggaagagccg caccgtcatc
     3781 gacgggccgt tcgccgagac gaaggagctc gtggcgggct tctggctgtg gcaggtgaag
     3841 tcgatcgaag aggccgtcga atgggcccgg cgttgcccga accccatgga gggcgagtcg
     3901 tatctcgaga tccgccccgt gttcgaggtc gcggacttcg gtgtcgagct cacgccggag
     3961 ctgcgcgctc aggacgctcg cctgcgtgcg cagaccgagg gcgaccgttc gcgctaatcg
     4021 gccgccaagc tgcttagacc cctccgcgga gggggctagc ttggcccgcg cttccggagc
     4081 cgcttcaccg agtgctcgat gagcttgcgt agcaccttgc ggtcgacgtc gtcgagcttg
     4141 ttgacgtaga ggcagccctt gccggtcttg tgcttgccga gcttcgccat gagcgtttcg
     4201 ttttcgatgc cggggcccag atagagaacc agcgcggcgg accgcggcga gaaacccgtc
     4261 atgcacatct cgccagaatg gccgctggcg taagtgtagc gatacaaccc gaaaccgatg
     4321 atcgacgagc cccacatctt cgccggctcg cccgtgattt cttgcatcat ctcgacgagc
     4381 tcgtagctgt cggcgcgctt ggcgtcgcct tcgattgtcg caatgaattt cttcgggtcg
     4441 gtcgcggtag cgaccgtttt tatcttgcgt ttactggcgg ccattggcgg ctccttcggc
     4501 gccggcggcg agcgcgctca acaatggcaa cagcagtgac gacgtgccga gtctcatgaa
     4561 gtgcgtcgtt attgcgtcgg cgtgcccggc ggcgcgaact cgatgacgat gctgcgtttg
     4621 gcgatgtgcc acgcgccgtc gaccttgcgc aacgtgtcct cgtagcggcc cgcgatcagc
     4681 acgccgggca agccgttgac gagaccgacg tcctgccaat acgcgccgcc cgtcgccgta
     4741 tcgccatcga tctcgtacga caggttcgtg atcggatgaa tcacgcgcgg ctccgcgggc
     4801 aatgccgaca cgaattgttt gatcgcggcg cggcctttcg aggtgttgcc ttgcagcgaa
     4861 agctcaccgt cgtccgtgaa cagcgcggcg tacgcgtcga agtcacgggt gtcgagcgcg
     4921 tgcgggtagt cgccggcgag caattgctcg atagcttggc ggtcgagcac gcgcgcgaga
     4981 ttcgggtcgg tccctcgtgc ggcgggttgg caggccgcga gggccatcgt tgcgagagcg
     5041 agcgtgccgg tgcgttgcag catggaggtg tccgaaggtt gcggggcagg cgagggcgtc
     5101 gacgttagca tgactcgacg cgcgtgcgca ccgcgggttt gacagtccgg cagcgacttc
     5161 ctaggctgcc ggcatgaaac aagatcccgt catcggcttc gcgccgctct cgttcccctg
     5221 gcaaacgaag gacccgttcc tcgtttgcgt ccatcacgtc gatctctatc cgcgcggcaa
     5281 cgaccggctt ggcccggcgg cgtcgctcga aggccgcaac atcggccaag acttcgccgg
     5341 caaggacggc tggcgcatgt accacggcga caccgtgccg ggttttccgc agcatccaca
     5401 ccgcggcttc gagacggtga cgttcgtgcg cgaaggcttc atcgatcaca gcgactcgct
     5461 cggtgcgacg gctcggttcg ggcgcggcga cgttcagtgg ctgactgccg gcgccggcat
     5521 cgtgcactcg gagatgttcc cgctgttgca gcccgatggc ggcaatcgtc tcgagctgtt
     5581 tcagatctgg gtcaacctgc ccgcgaagtc gaagttcgcg gcgccgtact tcacgatgtt
     5641 ctgggacagg gacgtgccgc ggcatcgtcc ggccgcgggc gtcgagctca ccatcgtcgc
     5701 cggcgctttc gcggacgcgc agccgcctgc gccgccaccg gactcgtggg ccgccaattc
     5761 cgacgccgat ctcgcgatct ggctgatcac gctcgaccgc ggcgcgtcgt ggacactgcc
     5821 gtccgccaag agcgcgcgca cgacgcgcac gttgtacttc ttctcgggcc cgaagctaga
     5881 cgtcgcgggg ctcacgctcg gccatcacgc ggcgatcgaa gtcgtggccg accaggccgt
     5941 gacgttgagc gcgcccgacg aacgcgccga agtgctgctg atgcaggggc ggcccatcgg
     6001 cgagcccgtc gcgcaatacg gcccgttcgt catgaacacg cgctccgagc tcgagcaggc
     6061 attcaacgac tatcggcgca cgcagttcgg cg
//
LOCUS       DATLEE010000234         2672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5484984, whole genome shotgun sequence.
ACCESSION   DATLEE010000234 DATLEE010000000
VERSION     DATLEE010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5484984"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VM692_04960"
     CDS             complement(<1..964)
                     /locus_tag="VM692_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51548.1"
                     /translation="MIMRTPTVLLAVAAVSIAGCGRGGNATATATPPDFSRSVTRERL
                     QNAASEPHNWLTHGNTYLEQRHSPLTQINKDTLSRLAPAWYYEFDTYRGQEATPLVVD
                     GVLYTTSAWSKAYALNAATGELIWSYDPKVPGAAGAKACCDVNSRGPALYDNKLIIAT
                     LDGRLIALDRATGMPIWSTQTVDATKMYTITGAPRVVKDKVVIGNAGAEFGVRGYVSA
                     YDADTGALVWRFYVVPGDPNASPDGAASDKALAEIAAPTWFGRWYEMGGGGTPWDAIV
                     YDVELDQLYIGTGNGSPWNRGVRSDGKGDNLFLASVLALDPDSGE"
     gene            complement(989..1315)
                     /locus_tag="VM692_04965"
     CDS             complement(989..1315)
                     /locus_tag="VM692_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51549.1"
                     /translation="MATFGFGQSTGQIVQTAYVDRRGYGFHHIGIAFEDVEAERVAYE
                     GRGYHVAFAAPVPSGGTVYYMGEGADAPGFVELIPATPGMDEMFTRYWRASVGWNGDD
                     PIRPFG"
     gene            1506..2534
                     /locus_tag="VM692_04970"
     CDS             1506..2534
                     /locus_tag="VM692_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HUQ51550.1"
                     /translation="MDITVLAYLEPDEKEPDVVVQQVADALAASGHKASILTIRHDLD
                     ELIDGLKARKPQLVFNLVESFGDDIMGGVIGVAGVLDLLQVPYTGGGPGEIFLQEDKA
                     LTKKLLAFEGIRYPDFATFAVDANFETGGNLRMPLFVKPLRMDASIGIDEKSLVRNTQ
                     QMMERVLYIQRTFGDAALAEEYIEGREFYVGVMGNDELTAFPPIEMDFSGLKEGSLKV
                     MDNSAKFDEKSDRFHGTKAIVASIEPELKAKLQKASIDAYRALRVCDYGRVDLRLTET
                     GEVYVIEVNANCYLEENSEFATAAAAHGLPYPKLVDAIVDLAVERGKHRRRAHKRRKK
                     ARSAAATT"
BASE COUNT          472 a          857 c          869 g          474 t
ORIGIN      
        1 attcgccgct gtcgggatcg agcgcgagca cgctggccaa gaacaagttg tcgcccttgc
       61 cgtcggagcg taccccgcga ttccagggcg agccgttgcc ggtgccgatg tacagctggt
      121 ccagctcgac gtcgtagacg atcgcgtccc agggggttcc gccgccgccc atttcgtacc
      181 agcggccgaa ccaagtcgga gctgcgatct cggcaagcgc cttgtccgac gcggcgccgt
      241 cggggctggc attcggatcg ccgggcacga catagaagcg ccacacgagc gccccggtgt
      301 ccgcatcgta agcgctgacg tagccgcgca cgccgaactc ggcgcccgcg ttaccgatga
      361 cgactttgtc tttgacgacg cgcggcgcgc ccgtgatcgt gtacatcttc gtcgcgtcga
      421 cggtctgcgt gctccagatc ggcatgccgg tcgcgcgatc gagagcgatg agccggccat
      481 cgagcgtcgc gatgatcagc ttgttgtcgt agagggccgg cccgcgcgag ttcacgtcgc
      541 aacaagcctt cgctccggcg gcgccgggca ctttcgggtc gtagctccaa atcagctcgc
      601 cggtcgcagc gttcaacgcg tacgccttgc tccaggcgct ggtcgtgtag agcacgccat
      661 cgacgacgag tggagtggct tcctggccgc gatacgtatc gaattcgtaa taccacgccg
      721 gcgccaagcg gctcagcgtg tccttgttga tttgcgtcag cggcgagtgc cgttgctcga
      781 ggtaggtatt gccatgtgtg agccagttgt gcggctcgga cgcggcgttc tgcaatcgtt
      841 cgcgcgtcac gctgcgcgaa aaatccggtg gtgtcgccgt cgcggtagcg tttcccccgc
      901 ggccgcagcc tgcgatcgac acggcggcca ctgcaagcag cacggtgggc gtgcgcatga
      961 tcatcggtgt ccccctcgac gcgcggcgtc agccgaacgg cctgatcgga tcgtcgccat
     1021 tccagccgac ggaggcccgc cagtatcgcg tgaacatctc gtccatgccc ggcgtcgcgg
     1081 gaatcagctc gacgaagccc ggcgcgtccg ccccttcccc catgtaatac accgtgccgc
     1141 cgctcggcac cggcgccgcg aacgcaacgt gataaccgcg gccctcgtaa gcaactcgtt
     1201 cggcctcgac atcctcgaac gcgatgccga tgtggtgaaa gccgtaaccg cgtcgatcga
     1261 cgtacgcggt ttgcacgatt tggcccgtgc tttggccgaa gccgaaggtc gccatgcaat
     1321 cgttatctca tggcgatcgc agctgcgcta ctggacgaag agctatctcg tcgcacgttg
     1381 cgacggcgaa acagccgtgt gatcccgtgc acgccatgag cgttatcgat tgtcgatcag
     1441 tgtcgcgtcg attagagtcg cgacaataac cgatcgcgag catcgcgagg gtgagctgac
     1501 ccggtatgga tatcactgtc ctcgcttact tggaaccgga tgagaaagag ccggacgtcg
     1561 tcgtgcagca agtcgccgac gcgttggctg cgtctgggca caaggcctcg atcctgacga
     1621 ttcgtcatga cctcgatgag ctcattgacg ggctcaaagc ccgcaagccg cagctcgtct
     1681 tcaacctcgt ggagtcgttc ggcgacgaca tcatgggcgg cgtcatcggc gtagccggcg
     1741 tgctcgactt gctgcaggtt ccgtatacgg gcggcgggcc cggcgaaatt ttcctacaag
     1801 aggacaaggc gctcacgaag aagctgctgg ccttcgaagg catccgctac cccgatttcg
     1861 cgaccttcgc cgtcgatgcg aacttcgaga cgggcggcaa cctgcgcatg ccgctgttcg
     1921 tgaagccgct gcgcatggac gcgtcgatcg gcatcgacga gaagtcgttg gtgcgcaaca
     1981 cgcaacaaat gatggagcgc gtgttgtaca tccagcgtac gttcggcgac gccgcgctcg
     2041 cggaggagta catcgaaggc cgcgagttct acgtcggcgt catgggcaac gacgagctca
     2101 cggcgtttcc gccgatcgag atggactttt cggggctcaa ggaaggcagc ctcaaggtca
     2161 tggacaacag cgcgaagttc gacgagaaga gcgaccgttt ccacggcacg aaagccatcg
     2221 tcgcgtcgat cgagccggag ctcaaagcga agctgcagaa ggcgtcgatc gatgcgtatc
     2281 gggcgctacg cgtctgcgac tacggccgcg tggatctgcg cctcacggaa accggcgagg
     2341 tctacgtcat cgaggtcaac gccaactgct acctcgagga gaacagcgag ttcgcgacgg
     2401 ccgcggccgc acacgggctg ccgtatccga agctcgtcga tgcgatcgtc gatctcgcgg
     2461 tggaacgcgg caagcatcgg cgccgcgcgc acaagcgccg caagaaggcg cgcagcgcag
     2521 cggccactac ctaatgcggc tcggcttttc gcagcggctc taggtcctgg ccgttgccgc
     2581 ccttgtcgaa aggcggtgcc ggcgtcgact cggcgctcgg cgcggctgac ccggccggcg
     2641 ccgccgcagg cggcggcgtc tccggaaagt ag
//
LOCUS       DATLEE010000235         2961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1013363, whole genome shotgun sequence.
ACCESSION   DATLEE010000235 DATLEE010000000
VERSION     DATLEE010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013363"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VM692_04975"
     CDS             complement(<1..778)
                     /locus_tag="VM692_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUQ51551.1"
                     /translation="MISAANITVQFGEKPLFEDVSVKFGNGNRYGLIGANGSGKTTFL
                     KVLGGELEPTHGNVSIAPNMRVGKLRQDQFAFEQFSVLHTVIMGHERLWQVYQERERL
                     YSKPSMTEEEGLHAAELENEFAQLDGYTAESAAGELLLAVGIPESQHLGPMSEVAPGW
                     KLRVLLAQALFANPDIMLLDEPTNNLDIDTIRWLETVLIERKCTMIIISHDRHFLNTV
                     CTHTADLDYRELRLFPGNYDEYMPAATQARERVHSENAKKK"
     gene            complement(896..1930)
                     /locus_tag="VM692_04980"
     CDS             complement(896..1930)
                     /locus_tag="VM692_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUQ51552.1"
                     /translation="MSSLLDRIARYGVAVLRTLLVCALLAGVTTSAQPPAPADVPAAE
                     PAAAEDAAPAWDFADEEEEAEPTLLEHARDQALDIGLFLAFATLAMVSFLRKSVPLKY
                     VTLVVAVLFMGRAKAYMLSVVNVFGVIHANLPIFANSMAWYAFAIFSIGTTVLWGRLY
                     CGRICAFGALTQLIDAVVPKRFQVEVPAKLERYGSYVKYGILLTAIGVYLATSEIAFY
                     RYIEPFWLFTFDATPVLWTMVGVLLVASIFVRNLYCRFLCPLGAALGLVSSVTTVFKI
                     KRWSECSQCALCEKTCEWGAIRKRKIIKSECVRCDDCEILYDDRRRCPHWLLELKRKA
                     RAAVTPRAAA"
     gene            complement(1927..2472)
                     /locus_tag="VM692_04985"
     CDS             complement(1927..2472)
                     /locus_tag="VM692_04985"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HUQ51553.1"
                     /translation="MRIALLAIGIVLLAVGASAQAPTEAALQGQLKKLFPTAATFSPK
                     GGSPAVYKAFGPGGENDLVGLAYWTTEIEPLERGFDGPIKMLVGLNTKGELTGVIVTD
                     HREPYGYFSVDLPEWPKQFEGKSVRDAFKVGTDVDAITRATISVTSASRAIRNSSRKV
                     ARAYLAPPPAAGTAPAATTAQ"
     gene            2582..>2961
                     /locus_tag="VM692_04990"
     CDS             2582..>2961
                     /locus_tag="VM692_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51554.1"
                     /translation="MQKHLAIALAGLAALSTAQAAFSQGGLDRRLAAAKRIECSFPTM
                     ATGTWDKNVPAIAVKPSELKANFFDINVDEGTAEAESAYGASFISVRYETGYLHIMQM
                     SDAGPLYLTTVLARAAGEGKLMAI"
BASE COUNT          511 a          936 c          988 g          526 t
ORIGIN      
        1 ctttcttctt cgcgttctcg gaatgcacgc gctcgcgcgc ctgcgtggcg gcgggcatgt
       61 actcgtcgta gttgccgggg aagaggcgca gctcgcgata gtccaagtcc gccgtgtgcg
      121 tgcacacggt gtttagaaaa tggcgatcgt gcgagatgat gatcatcgtg cacttgcgct
      181 cgatgagcac ggtctcgagc cagcggatgg tgtcgatgtc gaggttgttc gtgggctcgt
      241 cgagcaacat gatgtcgggg ttcgcgaaca gcgcctgtgc gagcagcacg cgcagcttcc
      301 agcccggcgc gacctcgctc atcgggccca aatgttgcga ctccgggatg ccgacggcca
      361 acaacagctc gccggccgcc gattcggccg tatagccgtc gagctgcgcg aattcgttct
      421 cgagctcggc cgcgtgcaag ccttcctctt ccgtcatcga cggtttcgag tacaggcgct
      481 cgcgctcctg atagacctgc cataggcgct cgtggcccat gatcacggtg tgcaggaccg
      541 agaactgctc gaacgcgaac tggtcctggc gcagcttgcc gacgcgcatg ttcggggcga
      601 tcgagacgtt gccgtgcgtg ggctcgagct cgccgccgag cactttcaaa aaggtcgtct
      661 tgcccgagcc gttggcgccg atcaagccat agcggttgcc gttgccgaac ttgacggaca
      721 cgtcctcgaa cagcggtttc tcaccgaact gcacagtgat gttggcggcg gaaatcatgg
      781 cgttccggtc ctgttccttg ggggtttggc cgcggcgccg atccatgcgc accgccggca
      841 gagggcgcgc agtataagtg atggctaagg cccggaaaag cgcaggttta tggggtcatg
      901 ccgcggcgcg cggcgtcacg gcggcgcgag ccttgcgctt cagctcgagc agccaatgcg
      961 ggcagcggcg cctgtcgtcg tagaggatct cgcagtcgtc gcagcgcacg cactcggact
     1021 tgatgatctt gcgcttgcgg atcgcgcccc attcgcaggt cttctcgcac aaggcgcatt
     1081 ggctgcactc ggaccagcgc ttgatcttga acaccgtcgt gacgctcgag acgaggccga
     1141 gcgccgcgcc aagcgggcac aggaaacggc aatagagatt ccgcacgaaa atgctcgcga
     1201 cgagcagcac gccgaccatc gtccagagca cgggcgtggc gtcgaacgtg aacagccaga
     1261 acggctcgat gtagcggtag aacgcgatct cgctcgtcgc gaggtacacg ccgatggccg
     1321 tgagcaagat gccgtacttc acgtagctgc cgtaacgctc gagcttggcc ggcacttcga
     1381 cttgaaagcg cttcggcacg acggcgtcga tcagctgcgt caacgcgcca aaggcgcaga
     1441 tgcggccgca gtagagccga ccccacagca ccgtggtgcc gatgctgaag atcgcgaacg
     1501 cgtaccacgc catgctgttt gcgaagatcg gcaggttcgc atggatcacg ccgaacacgt
     1561 tgacgaccga cagcatgtag gctttcgcgc ggcccatgaa cagcacggcc accacgagcg
     1621 tcacgtactt cagcggcacg ctcttgcgta ggaagctcac catcgcgagc gtcgcaaacg
     1681 cgaggaacaa gccgatgtcg agcgcttggt cgcgtgcgtg ctcgagcaac gtcggctcgg
     1741 cttcttcttc ctcgtccgcg aagtcccagg cgggcgctgc gtcttcggcg gccgcgggtt
     1801 ccgcggcggg gacgtcggcc ggcgccggcg gttgcgcgct cgtcgtgacg ccggccaaca
     1861 gcgcgcaaac gagcagcgtg cgcaacacgg ccacgccgta gcgcgcgatt cggtcgagca
     1921 acgagctcac tgggctgttg tggcggcggg cgccgtgccg gccgcgggcg gcggcgcgag
     1981 ataggcgcgc gcgaccttgc gcgacgagtt gcggatggcg cggctcgcgc tcgtgacgct
     2041 gatcgtcgcg cgcgtgatcg catcgacgtc ggtgccgact ttgaacgcgt cgcgcacgct
     2101 cttgccttcg aattgcttcg gccattcggg gaggtcgacc gagaaatagc cgtacggctc
     2161 gcgatggtcc gtgacgatga cgcccgtcag ctcgcctttc gtgttgaggc cgacgagcat
     2221 tttgatcggg ccgtcgaacc cgcgctcgag cggctcgatc tccgtcgtcc agtaggcgag
     2281 accgacaaga tcgttttcgc cgccggggcc gaacgctttg tacacggccg gcgagccgcc
     2341 cttcggcgag aacgtcgcgg ccgtggggaa cagcttcttg agctgacctt ggagcgcggc
     2401 ctcggtcgga gcttgagcgc tcgctccgac tgcgagtagc acgatgccga tggcgagtaa
     2461 ggcgatgcgc aaaggtcttc tcctcggtgc gtcgattatc agggcccctg ccgatgcggg
     2521 caacggtcaa gtttgctcga cccgcgtggc caacccgggg ccgacgtgct aaatactccg
     2581 catgcaaaag cacctcgcga tcgccctggc cggcttggcc gctttgtcga cggctcaggc
     2641 cgcatttagt caaggcggtc tcgaccggcg cctcgctgcg gcgaagcgca tcgagtgcag
     2701 tttccccacg atggctaccg gcacgtggga caagaacgtg ccggcgattg ccgtcaagcc
     2761 cagcgagctg aaagccaatt tcttcgacat caacgtcgac gaaggcacgg ccgaggccga
     2821 aagcgcgtac ggcgcgtcgt tcatctccgt gcgctacgaa accggttatt tgcacatcat
     2881 gcagatgagc gacgccgggc cgctgtacct gacgacggtg ctcgcgcgcg cggcgggcga
     2941 gggcaagctc atggccattc a
//
LOCUS       DATLEE010000236         4155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3121671, whole genome shotgun sequence.
ACCESSION   DATLEE010000236 DATLEE010000000
VERSION     DATLEE010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3121671"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VM692_04995"
     CDS             <1..1049
                     /locus_tag="VM692_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ51555.1"
                     /translation="ARLELAGKPWGYVYVILIGEAREVLAADVAATNVLDTTLTSMAV
                     IAVLAVLAGLIAFRLITRPLRGLARAMQELDVDRLAAAGAPAVPDVPDVPDERDETGI
                     LRDAFGRMAQRIADQWRTLRRQEQERREIIANVSHDLRTPLTSLHGFLETLAHKQDLS
                     AAERERYFKTALAQSDKVARLAHELFELARLEHDASSLEREPFSLGDLVHDVVQQLGM
                     LAAAKRQRLTANVPASLPNVEGSLRLIDRALMNLIGNAIRHSPEGGEIAIELRPVDGG
                     VEVVVRDEGPGIPAEVRETLLGTPAFGGRRVATGLGLQIVRRILELHGSRMELDDSAR
                     GTCMRFELKAAAPL"
     gene            complement(1110..2084)
                     /locus_tag="VM692_05000"
     CDS             complement(1110..2084)
                     /locus_tag="VM692_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HUQ51556.1"
                     /translation="MKQGLGVVLAIALWSCARAQAAEPLRQIATIALPGVAGRIDHLA
                     FDSQRQRLFVAALGNDTVEVLDTAGNSHVRSLQGFHEPQGIAVAADFGAVAVANGDTG
                     TLQLIDAETFATRWTVDIGGDADNVRYDAAAKQLYVAAEGGLYRVEPATGKVGARIAI
                     DGHPESFQLEAAGTRVFANLPSSEVVVGDRQRAAVVARWSSLGCRSNYPMAVDGTTMR
                     VLIGCRQPARLAVLDDRTGGVLTSAEIVGDTDDLFFDEQRQRIYVIGGEGFVDVLQRR
                     GDAVERTTRVGTRAGARTGLWVGSLSRLYVAVPARDGKAAEVRVLDAE"
     gene            complement(2099..2584)
                     /locus_tag="VM692_05005"
     CDS             complement(2099..2584)
                     /locus_tag="VM692_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUQ51557.1"
                     /translation="MERIEKIIEVDRPVRTVYNQWTQFEEFPRFMDGVKEVRQLDDTH
                     VHWHAEVFGKEKEWDAEITEQVPDEKIGWKSISGDAPNAGTVRFEPLGPSRTRVRLIM
                     AYEPQSAVESVGDALGLFSSRVEKSVKEFKRYIESRGAEDGAWRGQVHDSVADPTSRK
                     H"
     gene            2675..3133
                     /locus_tag="VM692_05010"
     CDS             2675..3133
                     /locus_tag="VM692_05010"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HUQ51558.1"
                     /translation="MEILDAYLMQIAKAAAYYLGLLIIVRLAGKRMAGQTTTFDLLIL
                     ITLGVVLQTTALQDGAANALIFVLTVFALHRGVALLCTRSRIVRDLLRGRPRPLVRDG
                     NVIEEALAAENVTRAELLAGLRKLGFARPEDVKLAVLEETGHISAVGRDP"
     gene            3189..3794
                     /locus_tag="VM692_05015"
     CDS             3189..3794
                     /locus_tag="VM692_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011790823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51559.1"
                     /translation="MIDNKQLQELLLQSLEHERGGVKVYETALKAALDDDLRKEWTGY
                     LSQTRKHVQILEGVLRELEIDAQRQTPGCEIVRGLGQALVDAMTKAQQAGNPAAAQLV
                     ACECVVIAETKDHMDWELLGKCAAQLQGKQRHALEQACESVEDEEDEHLYHSAGWTRE
                     LWLESLGLEAVLPPPEEQKDVKTAMAAAQAKQESERSRTTS"
BASE COUNT          706 a         1395 c         1362 g          692 t
ORIGIN      
        1 ccgcgcggct cgagctcgca ggcaaacctt ggggctatgt ctacgttatt ttgatcggcg
       61 aagcgcgcga agtgctcgcg gccgacgtcg cggcaacgaa cgtgctcgac acgacgttga
      121 cttcgatggc cgtcatcgcg gtccttgccg tgctcgccgg cctcatcgca ttccgcttga
      181 tcacgcgtcc gttgcgcggc ctcgcgcggg cgatgcagga gctcgacgtc gatcgcctcg
      241 ccgcggccgg cgcaccggcc gtccccgacg ttcctgacgt tcccgacgag cgcgacgaga
      301 ccgggattct gcgcgatgcc ttcggtcgca tggcgcagcg catcgcggac caatggcgca
      361 cgctgcggcg tcaggagcag gagcgccgcg agatcatcgc gaacgtgtcg cacgacctgc
      421 gcactccgct gacgtcgctg cacggctttc tcgaaacact ggcgcacaag caagacttga
      481 gcgcggcaga gcgcgagcgc tacttcaaga ccgcgctcgc gcagagcgac aaggttgcgc
      541 gcctcgcgca cgagctgttc gagctcgcgc gcctcgagca cgatgcctcg agcctggagc
      601 gggagccgtt ctcgctcggc gacctcgtgc acgacgtggt gcagcagctc ggcatgctgg
      661 cggccgcgaa gcggcagcgg ctcacggcca acgtgcccgc gagcctgccg aacgtcgaag
      721 gctcgctgcg cctcatcgat cgcgcactga tgaacttgat cggcaacgcg atccgccatt
      781 cgccggaggg cggcgagatc gcaatcgagc tgcgtccggt cgacggcggt gtcgaggtcg
      841 tcgtgcgtga cgaaggcccg ggcatcccgg ccgaggtacg cgaaacgctg ctcggaactc
      901 ccgcgttcgg cgggcgccga gtcgcaaccg ggctcggctt acaaatcgtg cggcgtattc
      961 tcgagctgca cggcagccgc atggagctcg acgactcggc acgcggcaca tgcatgcgct
     1021 tcgagctcaa agccgccgcg ccactctgag cgcacccggt ttcctgcggc gcgatccgcc
     1081 acgcggcgat cagtagactc ccgcgcgtct cattctgcgt ctaggacgcg aacttccgcc
     1141 gccttgccgt cgcgagccgg cacggccaca tagagcctgc tcagcgagcc gacccataag
     1201 cccgtgcgag cgccggcgcg cgtgccgacg cgcgtcgttc gctcgacggc atcgccgcgt
     1261 cgctgcagca cgtcgacgaa gccctcgccg ccgatcacgt agattcgctg ccgctgctcg
     1321 tcgaagaaca gatcgtcggt atcgccgacg atctcggccg acgtcagcac accgcccgtt
     1381 cgatcgtcga gcacggccaa gcgggccggc tgacggcagc cgatcagcac gcgcatcgtc
     1441 gtcccgtcca cggccatcgg gtagttcgag cggcagccga gagaagacca gcgcgcaacg
     1501 acggcagccc gctgtcgatc gccgactacg acctcggacg acggcagatt ggcgaatacg
     1561 cgtgtgcctg cggcttcgag ctggaacgat tcgggatgac cgtcgatcgc gatgcgcgcg
     1621 ccgaccttgc ccgtggccgg ctcgacgcga tagaggccgc cctcggctgc gacatagagc
     1681 tgcttcgcgg cggcgtcgta gcgcacgttg tcggcgtcgc cgccgatgtc gacggtccag
     1741 cgcgttgcga atgtttccgc atcgatgagc tgcagcgtgc cggtgtcgcc gttggcgacg
     1801 gcgacggcgc cgaagtcggc agcgacggca atgccttgcg gctcgtggaa gccctgcaac
     1861 gagcgcacgt gcgagttgcc cgcagtgtcg agaacctcga cggtgtcgtt gccgagcgcc
     1921 gcgacgaaca ggcgttgccg ctgcgagtcg aacgccaagt gatcgatgcg gccggcaacg
     1981 ccgggcagcg cgatcgttgc gatctgtcgc agcggctccg ctgcttgcgc gcgcgcacag
     2041 gaccacagcg caatcgccag gaccacgccg agtccttgct tcatgccgct actctaacct
     2101 agtgcttgcg cgacgtcgga tcggcgacgc tgtcgtgcac ttggcctcgc catgcgccgt
     2161 cctcggcacc gcggctttcg atgtagcgct tgaactcctt gacgctcttc tcgacgcggc
     2221 tgctgaacaa gccgagcgca tcgccgacgc tctcgacggc gctttgaggc tcgtaggcca
     2281 tgatcaatcg cacgcgcgtg cggcttggac ccagcggctc gaagcgcacg gtgcccgcgt
     2341 tcggggcgtc gcccgagatg ctcttccaac cgatcttctc gtccggcacc tgctccgtga
     2401 tctcggcgtc ccactccttt tccttgccga acacctcggc atgccaatgg acgtgcgtgt
     2461 cgtcgagttg ccggacctct ttgacgccgt ccatgaagcg cgggaattcc tcgaattgcg
     2521 tccactggtt gtagaccgtt cgcacgggtc tatcgacctc gatgatcttt tcgatgcgtt
     2581 ccatgctcgt ttcctcctgt cttagtggcg cggcgaacgt gcaagaaccg ttccgaccca
     2641 cgaggtctgg catggagctt gctccccatg gcgcgtggaa attctcgacg cttatctcat
     2701 gcagatcgcc aaagcggccg cctattacct gggcctgctg atcatcgtgc gcctcgccgg
     2761 caaacgcatg gccgggcaga cgacgacctt cgacctcttg atcctgatca cgctcggcgt
     2821 cgtgctgcaa acgacagcgc tccaagacgg cgctgccaac gcgttgatct tcgtgctcac
     2881 ggtgttcgcg ttgcaccgcg gcgttgcgct gctgtgtacc cgctcgcgaa tcgtgcgcga
     2941 tctcttgcgc ggcaggccgc gaccgctcgt gcgcgacggc aacgtgatcg aggaggcgct
     3001 cgcagcggag aacgtgacgc gcgcagagct actcgctggg ctacgaaagc tcggcttcgc
     3061 gcgtcccgag gacgtcaagc tcgcggtgct cgaggaaacg ggccatatct cggccgtggg
     3121 ccgcgatccc taggcatgtc acttgcacga gcaacgagcc aaggacattt cgcacaagga
     3181 ggaaagacat gatcgacaac aaacaactgc aggagctctt gctgcaatcg ctggagcacg
     3241 agcgcggcgg cgtcaaggtg tacgagacgg ccctgaaggc ggcgctggac gacgacctgc
     3301 gcaaggagtg gacggggtat ctgtcgcaaa cgcgcaagca cgtgcagatt ctcgaaggcg
     3361 ttctgcgcga gctcgagatc gacgcgcagc ggcaaacgcc gggatgcgag attgtgcgcg
     3421 gcctcggcca agcgctcgtc gacgcgatga cgaaggcgca gcaagccggc aacccggcgg
     3481 cggcacagct agtcgcttgc gaatgcgtcg tcattgccga aacgaaggat cacatggatt
     3541 gggagctgct cggcaagtgc gccgcgcagc tgcaaggcaa gcaacggcac gcgctcgagc
     3601 aagcgtgcga gagcgtcgag gatgaagagg acgagcacct ttaccacagc gccggctgga
     3661 cgcgggagct gtggctagag tcgctcggcc tcgaagccgt gctacctccg cccgaggagc
     3721 agaaggacgt caagacggcg atggccgcgg cgcaagccaa gcaagagagc gagcgcagtc
     3781 gcaccacgag ctgacgtttg acgaaggtgt cgcgcggcgg tccggacgcg cgcggttggc
     3841 gcccgaccgc gccgacaccg cactcgtcat cctagatctg atctcagact ttgcattcga
     3901 cgacggcgtc gcccgccgcg tcgccaagct caaggcgcgc ccgaaggccg ccggcattcc
     3961 cgcgattcga gttgcagccg caggcacgag ttgtgcatag aggtgggtgc ggatacgccg
     4021 acgtttgttc actcaatcca acgccttccg catccaaaag ccactcggct gctcgccggc
     4081 cgagtggctt gttcattttc gcgcgagctc gatccaaacg ggcgcgtgat cgctggcctt
     4141 gtcgcggccg cggac
//
LOCUS       DATLEE010000237         2308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6095964, whole genome shotgun sequence.
ACCESSION   DATLEE010000237 DATLEE010000000
VERSION     DATLEE010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6095964"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1246)
                     /locus_tag="VM692_05020"
     CDS             complement(221..1246)
                     /locus_tag="VM692_05020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUQ51560.1"
                     /translation="MKRIGFLSFGHWTPSPQSQVRSAADALLQSIELAVAAEQLGADG
                     AYFRVHHFARQLGSPFPLLAAVGARTSKIEIGTAVIDMRYENPLYMAEDAGAADVIAG
                     GRLQLGISRGSPEQVIDGWRYFGYAPQEGESDTDMARRHAEVFLEVIKGQGFAQPNPR
                     PMFPNPPGLLRVEPYAEGLRERIWWGSSSNATAQWAAQLGLNLQSSTLKTDESGEPLH
                     VQQRKQIEAYREAWKTAGHKREPRISVSRSIFALVDDRDRAYFGGRGGDEQDQIGYID
                     PTTRAIFGRSYAAEPDVLVKQLAADEAIAAADTLLLTVPNQLGVEYNAHAIESILRHV
                     APALGWC"
     gene            complement(1278..>2308)
                     /locus_tag="VM692_05025"
     CDS             complement(1278..>2308)
                     /locus_tag="VM692_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HUQ51561.1"
                     /translation="KADADGVRQFYGVARYGGQGRGVLYRISEGGVFKALHQLPASWG
                     ATGRTPSTTLLEVDGAFYGTTYQGGMNDGGVLFRMSDVDLPPVAQLSRPPSFTAGTLA
                     ASQSVPWHGQAQPITIEVFTGVAAWQAANTGAELLEPKPVPGLTDDGIKIRVSNCRNP
                     HVLQFVYREKLAADGTPSGAMYSTSSGESYAFTADPNKPNWHTDAGAGRPNPYYDQAG
                     GSAPSYSGSARIVGPFGLTMFDQPTFGEGLLFAPGARETWRATFKSYVICNCQVVREI
                     HWAREVPWIVDLSGTPTVIPTPTNGKQGPPRYTGVQIVETTDADLAWANAQIAADGFT
                     PITVIAAQ"
BASE COUNT          370 a          769 c          759 g          410 t
ORIGIN      
        1 gcgtacgatg gcaacgcgat cgcgacagcg gccgcgccga ccgccgccag cgccaaccat
       61 cgagtcacgc gcaccgtccg catcgagttc cttccttgag ggctcggcgc gatgcacgtt
      121 ttatgccggg acaaaatcgc cgcgacggcg ttgcacgcgt gcgttagcga cgcatttctt
      181 gggcgctttt acaaactcgg agaagtggcg cgaacgcgac tcaacaccag ccgagtgccg
      241 gcgcaacgtg ccgcagaatg ctctcgatcg cgtgcgcgtt gtactcgacg ccgagctggt
      301 tcggcaccgt caacagcagc gtgtcagcgg cggcgatggc ctcgtctgcg gcgagttgtt
      361 tgacgagcac gtcgggctcg gctgcgtacg agcggccgaa gattgcgcgc gtcgtggggt
      421 cgatgtagcc aatttggtct tgctcgtcgc ctccgcggcc gccgaagtac gcgcggtcgc
      481 ggtcgtcgac gagcgcgaag atgctgcggc tcaccgagat gcgcggctcg cgcttatgcc
      541 ccgccgtctt ccatgcctcg cgataggcct cgatttgctt gcgctgctga acgtgcaaag
      601 gctcgccgct ctcgtcggtc ttcagcgtcg agctctgtag gttcaagccg agctgcgcgg
      661 cccattgcgc cgtggcgttc gagctcgagc cccaccagat gcgctcgcgc aatccctcgg
      721 cgtacggctc gacgcgcagg agtccgggcg gattcgggaa cattgggcgc gggttcggtt
      781 gcgcgaaccc ctgccctttg atcacctcga ggaagacctc ggcgtggcga cgagccatat
      841 cggtgtcgct ttcgccctct tgcggcgcgt agccgaagta gcgccagccg tcgatcacct
      901 gttccggaga cccgcggctg ataccgagct ggagccgacc gcctgcgatg acgtcggcag
      961 caccggcgtc ctccgccatg tacagcggat tctcatagcg catgtcgatg acggccgtgc
     1021 cgatctcgat cttgctcgtt cgcgcgccta ccgccgcgag cagcgggaac ggcgagccca
     1081 gctggcgcgc gaagtgatgg acgcggaaat acgcgccgtc cgcgccaagc tgctcggcgg
     1141 cgacggcgag ctcgatcgat tgcagcaaag cgtccgccgc gctgcgcacc tgcgactgcg
     1201 gcgagggcgt ccagtgcccg aacgatagaa aaccgatcct tttcattgct gtccttttct
     1261 gcgaacgccg cgcgccgtca ctgtgcggcg atgaccgtga tcggcgtgaa gccgtcggcc
     1321 gcgatctgcg cgttggccca tgcgagatcc gcgtcagtcg tttcaacgat ctgcacgcca
     1381 gtgtagcgcg gcggtccctg cttgccgttc gtcggcgtcg gaatcaccgt gggtgtgccc
     1441 gaaagatcga caatccacgg gacctcgcgc gcccagtgaa tctcgcgcac gacctggcag
     1501 ttgcagatca cgtagctctt gaacgtggcg cgccacgttt cgcgggcgcc cggcgcaaac
     1561 agcagccctt cgccgaaggt cggctgatcg aacatcgtca gcccgaacgg gccgacgatg
     1621 cgagcgctgc cggaataact cggcgcggag ccgccggcct ggtcgtaata agggttaggg
     1681 cggccggcgc cggcgtcggt gtgccaattc ggcttgttcg gatcggccgt gaacgcgtac
     1741 gactcaccgc tcgacgtcga gtacatcgca ccggaggggg ttccgtccgc tgccagcttc
     1801 tctcgataca cgaactgcag aacgtgcgga ttgcggcaat tggagactcg aatcttgatg
     1861 ccgtcgtcgg tgaggcccgg taccggtttc ggctcgagca gttccgcgcc ggtattcgcg
     1921 gcctgccaag cagcgacgcc ggtgaacact tcgatcgtga tcggctgagc ttgtccgtgc
     1981 cagggcacgc tttgcgacgc agcgagcgtt cccgcggtga aggacggtgg acgcgacaat
     2041 tgtgcgacgg gcggcagatc gacgtcgctc atgcgaaaca gcacgccgcc gtcattcatg
     2101 ccgccttgat acgtcgtgcc gtagaacgcg ccgtcgacct cgagcaacgt cgtcgacggc
     2161 gtacggcccg tcgcccccca cgaggccggc agctgatgca gtgccttgaa gacgccgccc
     2221 tcgctgatgc gatacaacac gccgcggccc tggccgccgt agcgcgccac gccgtagaac
     2281 tgccgcacgc cgtcggcgtc cgccttct
//
LOCUS       DATLEE010000238         2236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1759737, whole genome shotgun sequence.
ACCESSION   DATLEE010000238 DATLEE010000000
VERSION     DATLEE010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759737"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2236)
                     /locus_tag="VM692_05030"
     CDS             complement(<1..>2236)
                     /locus_tag="VM692_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HUQ51562.1"
                     /translation="GPDGSGEWLPAPELFATPARELKYVLRAYGSGGTFDETVPQPLW
                     IAYRDGLPTKPEPQPTQDAVDALFSEVTTADAPYIDDFIDATVAEPAAPPLPPATEMP
                     TTDAGLRVAYGENNLGLSNIPLASGSVTVRGSAVPAGHSVYVAGRAVPVDSNGSFVAQ
                     EILPSGVHTVEVAILDEQGNGNLYLRDIELERNDWFYVGMADLTLTNNDTNGPVELLQ
                     GENSTYDFDSSADARLAFFVNGKFNEGWKLTASADTRDEPLKDLFSNFMNKSPDSLFR
                     RIDPDYHFPTFGDDSTVEEMAPTLGKFYVKAGRGDNYGEWGNFQIGYMNNELAQVDRG
                     LYGANMHFESKRATSFGERRFGIDGFTAEPGTIASREEFRGTGGSLYFLRRQDLLPGS
                     ERVRIELRDRASGLVTGVVNLTPAIDYEIDYLQGRVLLAEPLSSTREDDLLVRDGTAI
                     RGDEAHLVVRYEYTPGFEELDALSVGAQGHYWFGERVKLGLTANTNDDGTADSGLEAA
                     DVTVRFSSDSWFKLQGAQTEGFLSNVMRSDDGGFGFAGLADSSFADADAGGYRADVSL
                     GLGDLWDGNRGRLTMYTQSLDGGYAAPGLQTLADTENYGATFQMPMTQRITLRAKSDH
                     REAALGLSASAHEIDVGYQLNDNWAVSTGVRADERRDDSLVVPLTQEQGERKDAVVQV
                     GYDSKRRWSTYGFVQDTTSIDGNREENGRVGAGGSYRISERLKVDAEVSDGDLGTGGK
                     LGTNY"
BASE COUNT          316 a          760 c          770 g          390 t
ORIGIN      
        1 gatagttcgt gccgagcttg ccacccgtgc cgagatcgcc gtccgagacc tcggcatcga
       61 ccttcaagcg ctccgagatc cgatacgagc cgcccgcgcc gacgcggccg ttctcctcgc
      121 ggttgccgtc gatcgacgtc gtgtcctgca cgaagccgta cgtgctccag cggcgcttcg
      181 agtcgtagcc gacttgcacg acggcgtcct tgcgctcgcc ctgctcttgc gtcaacggca
      241 cgacgaggga gtcgtcgcgg cgctcgtcgg cgcgcacgcc ggtgctgacg gcccagttgt
      301 cgttgagctg atagccgacg tcgatctcgt gcgcgctcgc actgaggccg agcgccgcct
      361 cgcggtgatc cgacttcgcg cgcagcgtga tgcgctgcgt catcggcatc tggaacgtcg
      421 cgccgtagtt ctcggtgtcg gccagcgttt gcaagccggg ggcggcgtaa ccgccgtcga
      481 ggctctgcgt gtacatcgtg aggcggccgc ggttgccgtc ccacaagtcg ccgagtccca
      541 agctgacgtc ggcacgatag ccgccggcgt cggcgtcggc gaacgacgag tcggcgagac
      601 ccgcgaagcc gaagccgccg tcgtcggagc gcatgacgtt cgacaggaag ccttcggtct
      661 gcgcgccctg cagcttgaac cacgagtccg agctgaagcg gaccgtgacg tcggcggcct
      721 cgaggccgct gtcggcggtg ccgtcgtcgt tcgtgttggc cgtgaggccg agcttgacgc
      781 gctcgccgaa ccagtagtgg ccttgcgcgc cgactgacag cgcgtcgagc tcctcgaagc
      841 ccggcgtgta ctcgtagcgc acgacgaggt gcgcctcgtc gccgcgaatc gccgtgccgt
      901 cgcgcacgag caaatcgtcc tcgcgggtcg aggagagcgg ctccgccagc aagacgcggc
      961 cttgcagata gtcgatctcg tagtcgatcg ccggcgtcag gttcacgacg cccgtcacga
     1021 ggcccgacgc gcggtcgcgc agctcgatgc gcacgcgctc cgaacccggc aggaggtcct
     1081 ggcggcgcag gaagtacagc gagccgcccg tgccgcggaa ttcttcgcgg ctcgcgatcg
     1141 tgccgggctc ggccgtgaag ccgtcgatgc cgaaccgccg ctcgccgaag ctcgtcgcac
     1201 gcttcgactc gaagtgcatg ttcgcgccgt agagcccgcg gtcgacctgc gcgagctcgt
     1261 tgttcatgta gccgatctgg aagttgcccc actcgccgta gttgtcgccg cgccccgctt
     1321 tgacgtagaa cttgccgagc gtcggcgcca tctcctcgac cgtgctgtcg tcgccgaacg
     1381 tcgggaagtg atagtcgggg tcgatgcggc ggaacagcga gtccggcgac ttgttcatga
     1441 agttgctgaa caggtccttc agcggctcgt cgcgcgtgtc ggcactcgcc gtcagcttcc
     1501 agccttcgtt gaacttgccg ttgacgaaga acgcgagtcg cgcgtcggcc gacgaatcga
     1561 agtcgtacgt gctgttctcg ccttgcagca gctctacggg tccgttcgtg tcgttgttcg
     1621 tcagcgtcaa atccgccatg ccgacgtaga accagtcgtt gcgctcgagc tcgatgtcgc
     1681 gcagatagag gttgccgttg ccttgctcgt cgaggatcgc gacctcgacc gtatggacac
     1741 ccgacggcag gatctcctgc gcgacgaagc tgccgttcga gtccacgggc acggcgcggc
     1801 ccgcgacgta gacgctgtgc ccggccggca cggcgctgcc gcgcacggtc acggagccgc
     1861 tcgcgagcgg gatgttgctg agccccaagt tgttctcgcc ataggccacg cgcaggccgg
     1921 cgtccgtcgt cggcatttcg gtcgccggcg gcagcggcgg cgcggcgggc tcggcgacgg
     1981 tcgcgtcgat gaagtcgtcg atgtacggcg cgtcggccgt cgtgacctcg ctgaacagcg
     2041 cgtccacggc gtcttgcgtc ggctgcggct cgggtttggt cggcaatccg tcgcgatagg
     2101 cgatccagag cggctgaggc accgtctcgt cgaacgtgcc gccgctgccg taggcgcgca
     2161 gcacgtactt gagctcgcgc gccggcgtcg cgaacagctc gggcgcgggc aaccactcgc
     2221 cggagccgtc ggggcc
//
LOCUS       DATLEE010000239         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4738925, whole genome shotgun sequence.
ACCESSION   DATLEE010000239 DATLEE010000000
VERSION     DATLEE010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4738925"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VM692_05035"
     CDS             complement(<1..412)
                     /locus_tag="VM692_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HUQ51563.1"
                     /translation="MAAKKIANPVVAIVGATGAVGVELLECLERRKFPLKELKLFASA
                     RSAGKTLKFRGKDLRVEELTEQSFAGVDIGLFSAGSGTSKKFAPAAVKAGTVIVDNSS
                     AFRMDAGTPLVVPEINRAEIFKHEGVIANPNCSAI"
     gene            755..1735
                     /locus_tag="VM692_05040"
     CDS             755..1735
                     /locus_tag="VM692_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51564.1"
                     /translation="MAWLVPALASLLAGCGGDAGTPVTGRPSTGYNVPRTAWGDPDLQ
                     GMWPIDKLNGTPVQRPESFGERRLLTDEEYAERVDRLRGLNARYDEEIASNKMGIGHW
                     AEMGEPNRLTSLMVEPANGRVPPLTAEGERRSAAMTSSWSNIPFDKASDFNALDRCIT
                     RGMPASMLPFMYNSGIEIMQAPGYVVIRLELVHETRIVPVDDRPPLEPEVVQWLGESR
                     GRFEGDTLVIETTNFNGESPMLIVGPGAKPLPTSRELHIVERLTRVADDTIDYEITVE
                     DPVVLTQPWKAAFPLQRDDDYRMFEYACHEDNSAIRNFIQASRFERRTHP"
     gene            1732..>2381
                     /locus_tag="VM692_05045"
     CDS             1732..>2381
                     /locus_tag="VM692_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51565.1"
                     /translation="MTRPGDPTKTDEHERRLARLEKALGEALARSEAADAMLEDQRQR
                     LKALGAGREETMRALTEVRQELKRVSQERDELRKKLERVDSVQTATIALPDDYAGPSA
                     DDMPSLEDLMGALGEIEQPGPLVAGHLHQRVAAPDDSEEMISPTLVFPEEFAEVADSS
                     GRTDRSKDNEAGVWRVLVLLDSERPIKYPLYKETMTIGRADIADIQIDNGFLSRLHA"
BASE COUNT          446 a          795 c          760 g          380 t
ORIGIN      
        1 tgatcgccga gcagttcggg ttcgcgatga cgccctcgtg cttgaagatc tccgcgcggt
       61 tgatctccgg cacgacgagc ggcgtgccgg catccatgcg gaaggccgac gagttgtcga
      121 cgatgacggt gccggctttg acggccgccg gagcgaactt cttcgacgtg ccgctgccgg
      181 ccgagaacaa gccgatgtcg acgccggcaa agctctgctc ggtcagctcc tcgacgcgta
      241 ggtccttgcc gcgaaacttc agagtctttc cggccgagcg cgccgaagcg aacagcttga
      301 gctccttcag cgggaacttg cgccgctcga ggcactcgag cagctcgacg ccgacggcgc
      361 ccgtggcgcc tacgatcgcg acgacgggat tagcaatctt cttcgcggcc atgtctgact
      421 ccaagcaaga gacgcttaaa acaaaaaagc cccgtcgggg tccggcgggg ctttgggttc
      481 gcttgcgtga atcgcttcag cgcgcgcaac tctccaaagc cctttggcga gctttgttcg
      541 ttgcgccttt gcgagtcgag cggttcacgg gcgcgaacct tagcttactc gccgtgcgtc
      601 gtaaagcctg cggcgcttcg gccggtgctc gcaaacacga gccttttcac agaattgcgg
      661 tttgcatccg tatacaaggt gagcgcagga tttgcactct cgaattgcga gtacgcctct
      721 gaagcccgaa gccattcatc cgcgacgcac gagtttggcc tggctcgttc cggcgctggc
      781 gagcctgttg gccggctgcg gcggcgacgc cggcacgccg gtaacgggcc gcccgagcac
      841 gggctacaac gtgccgcgca cggcctgggg cgatcccgac ctgcagggca tgtggccgat
      901 cgacaaactg aacggcaccc ccgtgcagcg gcccgagagc ttcggcgagc gacgcctgct
      961 gaccgacgag gaatacgccg agcgagtcga tcgcctgcgc ggcttgaacg cgcgctatga
     1021 cgaagagatc gccagcaaca aaatgggcat cggccattgg gccgagatgg gcgagccgaa
     1081 tcgcctcacg tcgctgatgg tcgagcccgc gaacggccgc gtgccgccgc tgacggccga
     1141 gggcgagcgc cgctccgcgg cgatgacgag cagctggtcc aacattccat tcgacaaggc
     1201 gtcggatttc aatgcgctcg accgctgcat cacgcgcggc atgccggcct cgatgttgcc
     1261 gttcatgtac aacagcggca tcgagatcat gcaagcgccg ggctacgtcg tgatccgcct
     1321 cgagctcgtg cacgagacgc gcatcgtgcc cgtcgacgac cgcccgccgc tcgagcccga
     1381 agtcgtgcag tggctcggcg aatcgcgcgg ccgcttcgaa ggcgacacgc tcgtcatcga
     1441 gacgacgaat ttcaacggcg agtcgccgat gctgatcgtc gggccgggcg ccaagccgtt
     1501 gccgacgagc cgcgagctgc acatcgtcga gcggctgacg cgcgtcgccg acgacacgat
     1561 cgactacgag atcaccgtcg aggatcccgt cgtgctgacg cagccgtgga aggccgcgtt
     1621 cccgctgcag cgcgacgacg actaccgcat gttcgaatac gcttgccacg aggacaactc
     1681 cgccattcgc aatttcatcc aagcgtcgcg cttcgagcgg cgcacacacc catgacgagg
     1741 ccaggcgacc cgaccaagac cgacgagcac gagcgcaggc tcgcgcgact cgagaaggcc
     1801 cttggcgaag ctttggcccg aagcgaagcc gcggatgcca tgctcgagga ccagcgtcag
     1861 cggctcaaag cgctcggcgc cggccgcgaa gaaaccatgc gagcgctgac cgaagttcgt
     1921 caggagctca agcgcgtcag tcaggaacgc gacgagctgc gcaagaaact cgagcgagtc
     1981 gacagcgtac agaccgccac gatcgcgttg cccgacgact atgcggggcc gtcagcggac
     2041 gacatgccgt cgctcgagga cctcatgggc gcgctcggtg agatcgagca gccggggccc
     2101 ctcgtcgccg gccatctcca tcagcgcgtc gccgcgcccg acgattccga agagatgatc
     2161 tcgccgacgc tcgtgttccc cgaggagttt gccgaggttg ccgactcctc cggccgcacc
     2221 gaccgctcca aggacaacga agcaggcgta tggcgcgtcc tcgtgttgct cgactccgaa
     2281 cggccgatca agtatccgct ctacaaggaa accatgacga tcgggcgcgc cgatatcgcc
     2341 gacattcaaa tcgacaacgg cttcttgagc cgcttgcacg c
//
LOCUS       DATLEE010000240         3663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5491749, whole genome shotgun sequence.
ACCESSION   DATLEE010000240 DATLEE010000000
VERSION     DATLEE010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5491749"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /gene="tig"
                     /locus_tag="VM692_05050"
     CDS             complement(<1..1080)
                     /gene="tig"
                     /locus_tag="VM692_05050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HUQ51566.1"
                     /translation="MNAVNSKSYEVSLEPSAGLERRMTVRVPTADIERAISARLAQVG
                     KTAKLKGFRPGKVPQKVVRQYYGGQVRDEVMTEVIRRTYSSAIAEQKLNPAGGPRIEP
                     LAAEGQEHFAYRATFEVYPEIELKPLGDLAIEVPSVAIEDSDLDAMIEKLRTQRASWK
                     AVERPSAEGDRVVVDFAGTIDGESFEGGEGKSVAIVVGSGQVLQDFDRALRGIQAGAN
                     TAATVLFPQDYPSKNLAGKTAAFSITVLRVEELELPQLDDEFATSFGLAGGASSLRGE
                     VRNNMERELKERLRAEIKTRVFDSLIRANQVVVPRALVDQEIATLQSDALRQMGTSDP
                     SQAPPRERFEALAQRRVTIGLLIQEL"
     gene            complement(1110..1194)
                     /locus_tag="VM692_05055"
     tRNA            complement(1110..1194)
                     /locus_tag="VM692_05055"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1158..1160),aa:Leu,seq:tag)
     gene            complement(1348..3387)
                     /locus_tag="VM692_05060"
     CDS             complement(1348..3387)
                     /locus_tag="VM692_05060"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HUQ51567.1"
                     /translation="MQRKQRLQLASVVALGLMLGACGGDEEEAAATTGNHAPTITGTP
                     TITVNQGTSYSFTPSAADSDGDALIYGIDAKPAWLLFNTASGLLSGTPTAGDVGTHRG
                     IVVWVSDGKSQTLLPAFDLTVVAPTANNRAPTISGTPATSVEVNAQYSFVPTASDPDK
                     QPLTFSIRNRPSWAAFDIATGRLQGAPAAVGTFADIAISVSDGQVAIPLPAFTIVVTT
                     PAVNRAPVISGTPMTTVQTGGAYSFVPTASDADGDALTFAITGRPSWAAFDTGLGRLS
                     GTPPAGTTGAFGNIVISVSDGARSAALPAFAITVTAATGNRAPTISGTPATTATQGSA
                     YVFQPTAADADGNSLTFSIANRPTWATFNTNTGALQGTPGATHIRTYSNIVISVSDGS
                     ASTALPAFSIAVASSNTPPTISGTPPTTATVGTQYTFTPTASDANGGTLTFSIVNRPS
                     WATFTSTTGRLQGTPAAGNVGTFADIRISVSDGQDTTALAPFAIAVSQPPNRAPAISG
                     TPSASVMQGTQYTFTPTASDPDGNTLTFSITNAPAWATFNTATGQLSGTPGAQHIGAT
                     SGIVITVSDGTLSAALPAFTLTVQAVATGSATLNWTPPTTNTDGTPLTHLAGYKIYWG
                     TTSGNYSNSVALMNPGLTSYVVTNLVPGTYFFVATAVNSTGAESVFSAAASKSVP"
     gene            complement(3447..>3663)
                     /locus_tag="VM692_05065"
     CDS             complement(3447..>3663)
                     /locus_tag="VM692_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51568.1"
                     /translation="FAAPLSRRGCTRRQATSVATAESFFVGVHRPDRTSVSAENLKTA
                     SDLSADDDGSHCRGSASTTLQGAPLLV"
BASE COUNT          568 a         1225 c         1213 g          657 t
ORIGIN      
        1 aagttcttgg atcaacaggc cgatggtcac gcggcgctgt gccagagcct cgaagcgctc
       61 acgcggcggc gcttggctcg gatcgctggt gcccatctgc cgcagggcgt cggactgtag
      121 cgtcgcgatc tcctgatcga cgagcgcgcg cggcaccacg acctgattgg cacggatcag
      181 cgagtcgaac acgcgcgtct tgatctcggc ccgcagccgt tccttgagct cgcgctccat
      241 attgttgcga acttcgccac gcagcgaact ggcgccgccg gccaagccga agctcgtcgc
      301 aaactcgtcg tcgagctgcg gcagctcgag ctcttcgacg cgcagcaccg tgatcgagaa
      361 ggctgcggtc ttgccggcga gattcttcga cgggtagtcc tgcggaaaca gcacggtggc
      421 cgccgtgttg gcgcccgcct gaataccgcg caacgcgcgg tcgaaatcct ggaggacctg
      481 gcccgagccg acgacgatcg cgacgctctt cccttccccg ccctcgaagg actccccgtc
      541 gatcgtaccg gcgaaatcga cgacaacccg gtcgccttcg gccgaggggc gctcgacggc
      601 cttccagctt gcacgttgag tgcggagctt ttcgatcatg gcgtcgaggt ccgagtcctc
      661 gatcgcgacg ctcggcacct cgatcgcgag gtcgccgagc ggcttcagct cgatctcggg
      721 gtaaacctcg aacgtcgccc ggtacgcgaa atgctcttgg ccttcggcgg ccagcggctc
      781 gatgcgcggg ccgcccgcgg ggttcaactt ctgctcggcg atcgcgctgg agtaagtcct
      841 gcggatgact tccgtcatga cctcgtcgcg tacctggcca ccgtaatact gccgcacgac
      901 cttctgcggc accttgccgg gcctgaaccc tttgagcttg gcggtcttgc cgacctgcgc
      961 gagccgcgcg ctgatcgcgc gctcgatgtc ggccgtgggc acccgcacgg tcattcgtcg
     1021 ttccagtccg gccgagggct cgagcgacac ctcatagctc tttgaattta ctgcgttcat
     1081 ttcacttttt tagtcgtcaa ctcgaggatt ggtgcgaaag gggagactcg aactcccacg
     1141 ggttgcccca ctggattcta agtccagcgc gtctaccaat tccgccactc tcgccccgac
     1201 ggcgatcctc acggcggcat tatagacacg ccgcctccgc gaatctccaa acggagccgt
     1261 ttggcctgca gacgacccgg ccgccttacg gtgaaggcac gctgcacagc ggcgacggtc
     1321 ggccggcgtg ggccggcctg ggtacggcta cgggaccgac ttgctggcag cggcggagaa
     1381 gacgctttcc gcgccggtgc tgttgacggc agtcgcgacg aagaaatagg tgcccgggac
     1441 caaattggtc acgacgtagc tcgtgagacc cgggttcatc agcgccacgg agttggagta
     1501 gttgcccgac gtggtccccc aatagatctt gtagccggcc aagtgcgtca acggcgtccc
     1561 gtcggtgttc gtggtcggcg gcgtccagtt cagcgtagcc gaaccggtcg cgacggcttg
     1621 cacggtaagt gtgaaggccg gcagcgccgc gctcagagtg ccgtcgctga cggtgatgac
     1681 gatgccgctc gtcgcgccga tgtgctgcgc gcccggcgtg cccgacagct gccccgtcgc
     1741 cgtattgaac gtcgcccacg ccggcgcatt cgtgatgctg aacgtcaacg tgttgccgtc
     1801 gggatcgctg gccgtcggcg tgaacgtgta ctgcgttccc tgcatcacgc tcgcacttgg
     1861 cgtgccgctg atcgccggcg ctcgattcgg cggctgactg acggcgatcg cgaacggcgc
     1921 cagcgccgtg gtgtcttggc cgtcgctgac gctgatcctg atgtcggcga acgtgcccac
     1981 gttgcccgcg gccggcgtgc cttgcagccg ccccgtcgtc gacgtgaacg tcgcccacga
     2041 gggacggttg acgatgctga acgtcaaggt tccgccattc gcgtcgctgg ccgtcggcgt
     2101 gaacgtgtac tgagtgccga cggtcgccgt cgtcggcggc gtgcccgaga tcgtcggcgg
     2161 cgtgttcgag gacgccacgg caatcgagaa ggccggcagc gcggtcgacg cgctgccgtc
     2221 gctgacgctg atgacgatat tgctgtacgt gcggatgtgg gtggcgcccg gcgttccctg
     2281 cagcgcaccc gtgttcgtat tgaaggtagc ccacgtcggg cgattcgcga tcgagaacgt
     2341 cagcgaattg ccgtcggcat ccgcggccgt cggctggaac acgtacgccg agccctgcgt
     2401 cgccgtcgtc gccggcgtgc cggagatcgt cggcgccctg ttgcccgttg cggccgtcac
     2461 tgtgatcgca aacgccggca gcgccgccga ccttgcaccg tcgctgacgg aaatcacgat
     2521 gttgccgaac gcgcccgtcg tacctgccgg cggtgttccg gagagtcttc cgagaccggt
     2581 gtcgaacgcc gcccagctcg ggcggcccgt gatcgcgaac gttagcgcgt cgccgtcggc
     2641 gtcgctcgcg gtcggcacga acgaataggc gccgccggtc tgcacggtcg tcatcggcgt
     2701 gccggagatc accggcgcgc ggttgaccgc cggcgtcgtc acgacgatcg taaacgcagg
     2761 caacgggatt gcgacctgcc cgtcgctgac cgagatcgca atgtccgcaa acgttccgac
     2821 ggcagccggc gcgccttgca gtcgccccgt cgcgatgtcg aaggcggccc agctcggacg
     2881 attgcggatc gagaacgtca gcggctgctt gtcgggatcg ctggccgtcg gcacgaagct
     2941 gtattgcgcg ttgacctcga ccgaagtcgc aggcgtaccc gagatcgtcg gcgcgcggtt
     3001 gttcgccgtc ggtgccacga ccgtcagatc gaacgccggc agcagcgtct gcgatttgcc
     3061 gtccgaaacc cagacgacga tcccacgatg cgttccgacg tcaccggccg tgggcgtgcc
     3121 cgagagcaac ccgctcgcgg tgttgaacag cagccacgcg ggcttcgcgt cgatgccgta
     3181 gatcagtgca tcgccgtcgg agtcggcggc cgatggcgtg aacgaatagc tcgtgccttg
     3241 attgaccgtg atcgtcggcg tgccggtgat cgtcggggcg tggttacccg tggtcgccgc
     3301 cgcctcttct tcgtcgccgc cgcacgcacc gagcatcagc ccgagcgcga cgacgctcgc
     3361 aagctgcaag cgctgcttcc tttgcatcct gaatccgccc ccacggtaga acgggcggga
     3421 gattccaact tccgtgccaa cgagcgctag acgagcagcg gcgcgccttg cagcgtcgtc
     3481 gacgccgaac cgcgacagtg cgaaccgtcg tcgtctgccg acagatcgct cgccgtttta
     3541 agattttctg cagagacgct cgtgcgatcg ggtcggtgaa cgccgacaaa gaaactctca
     3601 gccgtggcaa cagaagtcgc ttggcggcgc gtgcacccgc gccgcgacag gggcgcggcg
     3661 aat
//
LOCUS       DATLEE010000241         2838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6846135, whole genome shotgun sequence.
ACCESSION   DATLEE010000241 DATLEE010000000
VERSION     DATLEE010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6846135"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..2001
                     /locus_tag="VM692_05070"
     CDS             85..2001
                     /locus_tag="VM692_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUQ51569.1"
                     /translation="MAKSGSRVDRRKFLRGAAAAALTAPALPAAAQPPAAEPSAPLSP
                     ALAAQTLAAETAALDAGADALTVANPGSDFIVDVLKTLDFEYVIANPASSFRGLHESF
                     INYGGNSAPEWLTCTHEQAAVNIANGYFAVAGKPMAVVTFAPSGLQHAAMGIFGSFSG
                     RTPTYLLCANIPGGEYRRPLFDWGPHAMTDPAAMVRDMLKWDDTPASLQHFAESAVRA
                     YRMAMTEPRGPVMLVVDAALQEEAIGDRAALRIPRLAMPSPPAGDAAAVAEAAKMLVA
                     ANAPLILAGDVARDEDGMRLLVELAETLQAPVQGGGRAMPNQHPLAGGGSIPNADVIL
                     ALNEDALYGRLNRYRDQQVRTSVPLVRPDAKIISISSHDLNVRSNYQNVERFQEVTLA
                     ISADPQATLPSLIDECNKLIAPGRRRALDERGKSLATASAAALERARVEATYGWDASP
                     ITLQRLAAEVWDLIKAKDWASVGAAGGRLWNADKFYRTMGSVNGGGIGGALPIAIGAA
                     LAHKKDGRLCVRFQPDGDMLYMATSLWTATHHRIPMLIVMHNNRAYHQEVMHLQRMAN
                     RRQRGVELAAHGLPGATLGDPDIDFAQMARSMGAYAEGPIADPNELRPALLRAIARVE
                     QGEVALLDTRTQAR"
     gene            2008..2364
                     /locus_tag="VM692_05075"
     CDS             2008..2364
                     /locus_tag="VM692_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUQ51570.1"
                     /translation="MRCTALKLVAASSLLWAASVVAQAPGGAAARGRGLFVDRGCWQC
                     HGLAAQGGGIAGPRLAGRVQAWAAFAPYVRRPTEEMIPYTAQVLPDSELADIFEWLRT
                     LPPPPPVETLPLLLGD"
     gene            complement(2368..2736)
                     /locus_tag="VM692_05080"
     CDS             complement(2368..2736)
                     /locus_tag="VM692_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUQ51571.1"
                     /translation="MAKALVASALLCCALGAAAQPSARNERAAAVNGQSVFLRLGCFT
                     CHGTVGHGGAAPRLTPNTLPLEAFTTWVRDGTPGWTVASGMPAFPTSVLSDAELADIR
                     AYLASLPAPPAVADVPLLAL"
BASE COUNT          456 a          971 c          992 g          419 t
ORIGIN      
        1 gcttgcatcg gcacgcggtt cggcgaacgc cccgaggctc tggcaggctt agaatctgaa
       61 tgcccaggcg gcaggggggg acccatggcc aaatcaggct cgcgcgtcga tcgacgcaaa
      121 ttcttgcgcg gcgcggcggc cgcagcactg acggcgccgg cgctgccggc agccgctcag
      181 cctcccgcgg ccgagccgtc ggccccgctc tcgccggcac tcgccgcgca aacgctggcg
      241 gccgagacgg cggcgctcga cgccggcgcc gatgcgctta ccgtcgcaaa cccgggctcc
      301 gacttcatcg tcgacgtgct gaagacgctc gacttcgagt atgtcatcgc gaatcccgcc
      361 tcgagcttcc gcggcttgca cgagtcgttc atcaattacg gcggcaactc tgcgcccgaa
      421 tggctgacgt gcacgcacga gcaggccgcg gtcaacatcg cgaacggcta ctttgccgtc
      481 gcgggcaagc cgatggccgt cgtcaccttc gcgccgtcgg gcttgcagca cgcggcgatg
      541 ggcattttcg gctcgttctc cggccgcact ccgacgtatc tcttgtgcgc caacattccg
      601 ggcggcgaat accggcggcc attgttcgat tgggggccgc acgcgatgac ggacccggct
      661 gcgatggtcc gcgacatgtt gaagtgggac gacacgccgg cgtcgctgca gcatttcgcg
      721 gagtcggccg tgcgcgccta ccgcatggcg atgacggagc cgcgtggccc cgtgatgctc
      781 gtcgtcgatg cggcgctgca agaagaggcg atcggcgacc gtgcggcatt gcgcatcccg
      841 cggctcgcca tgccgagccc gcccgcgggc gatgcagcgg ccgtggcgga agcggcgaag
      901 atgctcgtag cggcgaacgc gccgctgatc ctggccggcg acgtcgcgcg cgacgaggac
      961 ggcatgcgac tgctcgtcga gctcgccgag acgctacaag cgcccgtgca aggcggcggg
     1021 cgcgccatgc cgaatcaaca tccactcgcg ggcgggggca gcattccgaa cgctgacgtg
     1081 atcctcgcgc tgaacgaaga tgcgctgtac ggccggctga accgctatcg cgaccagcaa
     1141 gtgcgcacgt cggtgccgct cgtcaggccc gacgcgaaga tcatcagcat ctcgagccac
     1201 gatttgaacg tgcgcagcaa ctaccagaac gtcgagcgtt tccaggaagt gacgctcgca
     1261 atctcggccg atccgcaagc gacgctgccg agcctcatcg acgagtgcaa caagctcatc
     1321 gcgcccggcc gtcgccgcgc gctcgacgag cgcggcaagt cgctggccac ggcatcggcc
     1381 gcggcgctcg agcgcgcgcg cgtggaagcg acgtacggtt gggacgcaag ccccatcaca
     1441 ctgcagcggt tggccgccga ggtttgggac ctcatcaagg ccaaagactg ggcgtcggtc
     1501 ggtgcggcag gcggacgctt gtggaacgcc gacaagttct atcgaacgat gggctccgtg
     1561 aacggcggcg gcatcggcgg cgcgttgccg atcgcgatcg gcgccgcgct cgcgcacaag
     1621 aaggacggac gcctgtgcgt gcgcttccag cccgacggcg acatgttgta tatggcgacg
     1681 tcgttgtgga ccgcgacgca tcaccgcatt ccgatgctga tcgtcatgca caacaatcgc
     1741 gcgtatcacc aggaggtcat gcacctgcag cgcatggcga atcgccgtca gcgcggcgtc
     1801 gagctcgcgg cgcacggctt gcccggcgcg acgctcggcg accccgacat cgacttcgcg
     1861 caaatggcgc gcagcatggg agcgtatgcc gaggggccga tcgcggaccc gaacgagctg
     1921 cgcccggcgc tgttgcgagc gatcgctcgc gtcgagcagg gcgaggtcgc actgctcgat
     1981 acgcgcacgc aggcgcggtg atatccaatg cgttgcacgg cactgaagct cgtcgctgcg
     2041 tcgtcattgc tctgggcggc gtccgtcgtg gcgcaagcgc cgggcggcgc tgccgcgcgc
     2101 ggccgcgggc tcttcgtcga tcgtggctgc tggcaatgcc acggtttggc ggcccagggc
     2161 ggcggcatcg cggggccgcg gctcgccggc cgagttcagg catgggcggc gttcgcgccg
     2221 tacgtgcgtc gcccgacaga agaaatgatt ccgtacaccg cgcaagtcct gccggatagc
     2281 gagctcgcgg acatcttcga atggctgcga acgctgccgc cgccaccgcc cgtcgaaacg
     2341 ctaccgctgt tgctcggcga ctgaccctca aagcgcgagc agtggcacgt cggcgacggc
     2401 cggcggcgcc gggaggctcg caagatacgc acggatgtcg gcgagctcgg cgtcgctcaa
     2461 cacgctggtc ggaaatgccg gcatgccgct cgcgaccgtc caacccggcg tgccatcgcg
     2521 gacccaagtc gtaaacgctt cgagcggcag cgtgttcggc gtgagccgcg gcgccgcgcc
     2581 gccatggccg accgtgccgt ggcacgtgaa gcagccgagg cgcaggaaaa cgctctgacc
     2641 gttgacggct gcagcgcgct cgttgcgcgc cgacggctgc gccgccgcgc cgagcgcgca
     2701 gcagagcagg gcggatgcga caagtgcctt cgccattcgc ggagctagcg cggctgcgtc
     2761 acgacgtcga tcaggtacgg ctcgccgcgc ttcacgacgt cgaggccgcg ccgaatcgcg
     2821 gcgctgagat ccttggga
//
LOCUS       DATLEE010000242         4991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1025219, whole genome shotgun sequence.
ACCESSION   DATLEE010000242 DATLEE010000000
VERSION     DATLEE010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025219"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VM692_05085"
     CDS             <1..1170
                     /locus_tag="VM692_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ51572.1"
                     /translation="TDRRLAEARLRASEEALRKADQRKDEFLATLSHELRNPLAPIRT
                     AAEMLTLPNLSDEQLAWPRQVIYRQVEHMARLLDDLLDVARITRGKLQLRFQPVDLGT
                     IVDTAVEAARPLITARKHGLTIELPPDLPTFAADPVRLAQVLSNLLTNAAKYTDSPGR
                     IALAARVEGGMLRIAVKDNGIGISPGDLPDIFEMSSQVQDGGSRAEGGLGIGLALVKG
                     LVTLHGGDIEVFSEGTGYGSEFVVTLPCEPPAAAQAAEVAPAAEAAATTVARKILVAD
                     DNQDAANTLAMLLRLSGHDVRTAHGGEAALTLASAFQPDIALLDIGMPDLNGYEVARQ
                     LRRTDCGKDLRLIALTGWGQDEDKRRALDAGFDHHLTKPVDPRRLDALLAPTVRD"
     gene            complement(1147..1743)
                     /locus_tag="VM692_05090"
     CDS             complement(1147..1743)
                     /locus_tag="VM692_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51573.1"
                     /translation="MLFQARFHERIVRGEIRCTLRIWQRPHVKVGGRYKLGAGTVVVE
                     RIYETRLDDVTPALARRCGFASVADALQTAKHGAGERVFVIDFHYDGAARQGPTTAVV
                     SAEELAELAQRLEAMDRRSRSGTWTLATLRAIEARPGVLAATLARSLGRPRDEFKRDV
                     RKLKGLGLTFSLEIGYRLTPKGAALLAARPSSIADGGG"
     gene            complement(1767..2675)
                     /locus_tag="VM692_05095"
     CDS             complement(1767..2675)
                     /locus_tag="VM692_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51574.1"
                     /translation="MSRFVLSCVALVGSLLALGSVQAQPPAQPRSVSCVRGGLQRAVD
                     LYIEAQTKGDTSGLPLVNGLGYVENMERLDIDAGVIKTALEIDHHRSLLDEATCQTFT
                     EVIVTDAAKPYVLGTRMRINHDKIAEIEILWTTTGYWLFNADNYLKYSSAEDWGPIPP
                     ERRDSRETLVAAANAYLDAFLEGKIDQVPWGFPCVRVEGGMYTGKGSPADSCEVGVPS
                     GVNIANRQFVVDDVIGSVVAYCTFGAGGPNGGSGSPDTHLFRVENGKLRYVHTLTHLL
                     QSAFRGGGGQNAGQNRTQGNGQTPER"
     gene            complement(2733..3647)
                     /locus_tag="VM692_05100"
     CDS             complement(2733..3647)
                     /locus_tag="VM692_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ51575.1"
                     /translation="MLSRRSFIGTSAAFVGGTLSSAFAARAQQAAAAVTTSELGGGLF
                     LFQGAGCNVVALRGDDGALMIDGGLAANADALLRAVLAATRNDLVATLINTHWHPEQT
                     GANALVGRAGGTILAQEKTAMYLGSRATSTLFEGRLEPLPEEGRPKQKTRAEGTLQFG
                     GQRVDYGYLPAAHTDGDLFVHFPQLNVLVAGGVVAAKSWPLLDYRNGAWFGGRVRALE
                     RLASLIKPDTRVVPADGALLTGRDIVRQRDILDELFVTMIGYMNMGLGAEDVVARNPL
                     QKYAAEFGDASKFLDGAFRSMQIAYVPD"
     gene            complement(3652..>4991)
                     /locus_tag="VM692_05105"
     CDS             complement(3652..>4991)
                     /locus_tag="VM692_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ51576.1"
                     /translation="VSLANDYGASPMSLAAEVGDAALLKVLLDAGANADSPSGDGMTA
                     LMLVARTGNVEAAKMLLDHGATIDARENFGGQTALMWAAARRHPEMIDLLVVRGAAID
                     ARSAVRDYQRHIQAEGRPKSLDSGGFTPLLYAARENCMACVDTLLEKGADIDLPDPDG
                     VTPLHVAIMNANWDLAKRLIEAGADVQQWDIYGEAPLFTAVGGRSRNDGGRASIDPPN
                     QATGLDIVKLLLERGANPNMQLFFRPANVNGATNTRGSTPLIRAAANNDLEVVKLLLE
                     HGADATVYMADRQTPIHAALAGRANEKDALELIKVLHDAGTDVNVVALIVHIQEVRGG
                     SALHYAVRKRYKEVIRLLASYGADMNLKDQDGLTALDYTQSRGFMPFMANQTPLYKDE
                     AALLRELGATVMMPKDPVWPVLGPPQGMWPDIWPLGEPVVHPPVYRHDSTPSG"
BASE COUNT          806 a         1676 c         1685 g          824 t
ORIGIN      
        1 acggaccggc gcctggcgga ggcccgtttg cgcgccagcg aagaggcgct gcgcaaagcg
       61 gatcagcgca aggacgagtt cctcgcaaca ctctctcatg agctcaggaa tccgctcgcg
      121 ccgatacgga cagcggccga aatgctgacg ctgccgaatc tcagcgacga gcagctcgcg
      181 tggccgcgcc aagtgattta ccggcaggtc gagcacatgg cgcggctgct cgacgatctg
      241 ttggacgtcg cgcgcatcac gcgcggcaag ctgcaactga ggttccagcc cgtcgacctc
      301 ggcacgatag tggatacagc cgtcgaagcg gcacggccct tgattacggc ccgcaagcac
      361 ggactgacga tcgagctgcc tccggacctg cccacgttcg ccgcagaccc ggttcggttg
      421 gcgcaagtgt tgtcgaacct gctcacgaat gccgcgaagt acaccgactc gccggggcgc
      481 atcgcgctgg cggcacgtgt cgaaggcggc atgctgcgga tagccgtcaa agacaacggc
      541 atcggcatct cgcccggaga tctgcccgac atcttcgaga tgtcctcgca ggtgcaggac
      601 ggcggcagcc gcgccgaggg cggactcggc atcggcctcg ccctcgtcaa gggcctcgtg
      661 actttgcacg gcggcgacat cgaagtcttc agtgaaggca ctggttacgg cagtgagttc
      721 gtcgtgacgc tgccgtgcga gccgccggcc gcggcgcagg cggccgaggt cgcgccggcc
      781 gccgaggctg ccgcaacgac ggtcgcacgc aagatcctcg ttgccgacga caaccaggat
      841 gcggccaaca cgctggccat gctgctgcgc ctgtcggggc acgacgtccg cacggcgcat
      901 ggcggcgaag cggcattgac gctcgcgagc gcgttccagc ccgacatcgc gctgctcgac
      961 atcggcatgc cggacttgaa cggctacgag gtcgcgcgac aactgcgccg caccgactgc
     1021 ggcaaggatc tgcgtctgat cgcgctcacg ggttggggac aggacgagga caagcgtcgc
     1081 gcgctcgatg ccggcttcga ccaccacctg acgaagcccg tggacccgcg caggctcgac
     1141 gcgctgctag cccccaccgt ccgcgattga cgacgggcgc gcggccaaca gcgctgcacc
     1201 tttcggcgtc aagcggtagc cgatctccaa gctaaacgtc agaccgagac ccttgagctt
     1261 gcgcacgtcg cgcttgaact cgtcgcgggg gcgacccagc gagcgcgcaa gcgtcgcggc
     1321 cagcacgccg ggtcgcgctt cgatcgcgcg caacgtggcg agagtccacg tgccgctgcg
     1381 cgagcgacgg tccatcgcct cgaggcgttg cgcgagctcg gcgagctcct cggccgacac
     1441 cactgccgtc gtcggtcctt ggcgtgctgc gccgtcgtag tggaaatcga tgacgaaaac
     1501 gcgctcgccg gcgccgtgct tcgcagtctg cagggcatcg gcgaccgagg cgaagccgca
     1561 gcgccgcgcc agcgccggcg tgacgtcgtc gagccgcgtt tcgtagatcc gctcaacgac
     1621 aacggtgccc gcaccgagtt tgtaacggcc gccgactttg acgtgtgggc gctgccagat
     1681 tcgcagcgtg cagcggatct cgccgcgcac gatgcgctca tgaaagcgtg cttggaacag
     1741 cattcgcgct gccgcgcggg cggcgcctag cgctcgggcg tttgcccgtt gccctgcgtc
     1801 cggttctgac ccgcgttttg cccgccgccg ccgcggaacg ccgattgcag caggtgcgtc
     1861 aacgtgtgca cgtagcgcag cttgccgttc tcgacgcgga acagatgggt gtcgggcgag
     1921 ccgctgccgc cgttcggacc gccggcgccg aacgtgcagt aggcgacaac cgagccgatg
     1981 acgtcgtcga cgacgaactg gcgattcgcg atgttgacgc cgctcggcac gccgacctcg
     2041 cagctgtcgg ccggcgagcc cttgccggtg tacatgccgc cctcgacgcg cacgcatggg
     2101 aagccccatg gaacctggtc gatcttcccc tcgagaaacg cgtcgaggta ggcgttcgcg
     2161 gccgcgacga gcgtctcgcg cgaatcgcgt ctctcggggg ggatcggccc ccagtcttcg
     2221 gccgacgagt acttcaagta gttgtcggcg ttgaacagcc aatagcccgt cgtcgtccag
     2281 agaatctcga tctcggcgat cttgtcgtgg ttgatgcgca tgcgcgtccc gagcacgtac
     2341 ggtttcgcgg catcggtcac gatgacctcg gtgaaggtct gacacgtcgc ctcgtcgagc
     2401 agactgcgat gatgatcgat ctcgagcgcc gtcttgatga cccccgcatc gatgtcgagc
     2461 cgctccatgt tctcgacgta accgagcccg ttgacgagcg gcagaccgga ggtgtcgcct
     2521 ttggtttgcg cctcgatgta gagatcgacg gcgcgttgca gcccaccgcg aacgcaagac
     2581 acggaccgcg gctgtgccgg cggttgcgcc tgcaccgagc ccagcgcgag cagcgaaccc
     2641 acaagcgcga cgcacgagag aacaaaacgg ctcactgcac ttctcctcgt ttctgtttgt
     2701 cgttgcggac gaggtcgatc gttgtcgctg atctagtccg gcacgtaggc gatctgcatg
     2761 ctgcggaacg cgccgtcgag gaacttggac gcatcgccga actcggcggc gtacttttgc
     2821 aacgggttac gcgcgacgac gtcttcggcg ccgaggccca tgttcatgta gccgatcatc
     2881 gtgacgaaca gctcgtccaa gatgtcgcgt tgccggacga tgtcgcggcc cgtcagcaac
     2941 gcgccgtcgg ccggcacgac gcgcgtatcg ggtttgatga ggctcgcgag ccgctcgagc
     3001 gcacgcactc ggccgccgaa ccacgcgccg ttgcggtagt cgagcaacgg ccatgacttt
     3061 gcggcgacga cgccgccggc cacgagcacg ttcagttgcg ggaagtgcac gaataggtcg
     3121 ccgtcggtgt gcgcggcggg cagatagccg tagtcgacgc gttgcccgcc gaattgcagc
     3181 gttccctctg cgcgcgtctt ctgtttcggc cggccttctt cgggcagcgg ctcgagccgg
     3241 ccctcgaaca gagtcgacgt cgcgcggctg cctagataca tcgcggtctt ctcttgcgcc
     3301 aagatcgtac cgcccgctcg gcccacgagc gcgttcgcac ccgtctgttc gggatgccag
     3361 tgcgtgttga tcagcgtcgc gacgagatcg ttgcgggtcg cggcgagcac ggcgcgcagc
     3421 aacgcgtccg cgttagccgc gagaccgccg tcgatcatca gcgcgccatc gtcgccgcgc
     3481 agcgcgacga cgttgcagcc cgcgccttgg aaaagaaaca acccgccgcc gagctcggac
     3541 gtcgtgacgg ccgcggccgc ttgctgcgca cgcgcggcaa acgcagaaga cagcgtgcca
     3601 ccgacgaacg cggcactcgt gccgatgaaa gagcgccgcg agagcacgcg cttagccgct
     3661 gggggtcgag tcgtggcggt aaacgggcgg atgaacgacc ggctcgccaa gcggccagat
     3721 gtccggccac atgccttgcg gcgggcccag caccggccag acggggtcct tcggcatcat
     3781 gaccgtggcg ccgagctcgc gcagcagcgc ggcctcgtct ttgtaaagcg gggtttggtt
     3841 cgccatgaac ggcatgaacc cgcgcgactg cgtgtagtcg agcgccgtga ggccgtcctg
     3901 atccttgagg ttcatgtcgg cgccgtacga cgcgagcagc cgaatgactt ccttgtagcg
     3961 cttgcgcacg gcgtaatgca gcgccgagcc gccgcgcact tcctggatgt gcacgatcag
     4021 cgcgacgacg ttcacgtccg tgcccgcgtc gtgcagcact ttgatcagct cgagcgcgtc
     4081 cttctcgttg gcgcggcccg cgagcgcggc gtgaatcggc gtttgccgat cggccatgta
     4141 gacagtcgcg tcggcgccgt gctcgagcag cagcttgacg acctcgaggt cgttgttggc
     4201 cgccgcgcgg atcagcggcg tcgagccgcg cgtattcgtc gcgccgttga cgttggcggg
     4261 acggaagaac agctgcatgt tcgggttcgc gccgcgctcg agcagcagct tgacgatatc
     4321 gagtcccgta gcttggttcg gcggatcgat cgacgcgcgg ccgccgtcgt tgcgcgaacg
     4381 tccgccgacg gccgtgaaca gcggggcctc gccgtagatg tcccactgct ggacgtccgc
     4441 gccggcttcg atcaagcgct tcgccagatc ccagttcgcg ttcatgatcg cgacatgcag
     4501 tggggtcacg ccgtcggggt ccggcagatc gatgtcggcg cccttctcga gcaacgtgtc
     4561 gacgcacgcc atgcagttct cgcgcgcggc atacagcagc ggcgtgaagc cgcccgagtc
     4621 gaggctcttg ggccgcccct cggcttggat gtgcctctga tagtcgcgaa cggccgagcg
     4681 cgcatcgatg gccgcgccgc gtacgacgag caagtcgatc atctcggggt ggcggcgcgc
     4741 ggcggcccac atcagcgccg tctggccgcc gaaattttcg cgggcgtcga tcgtcgcgcc
     4801 atggtcgagc agcatcttcg cggcttcgac gttgcccgtg cgtgccacga gcatcagagc
     4861 ggtcatgccg tcgccactcg gcgagtcggc attcgcaccg gcgtcgagca gcaccttcag
     4921 tagcgcggca tcgccgacct cggccgcaag gctcatcggt gaagcgccgt agtcgttggc
     4981 gagcgagacg t
//
LOCUS       DATLEE010000243         2105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7590761, whole genome shotgun sequence.
ACCESSION   DATLEE010000243 DATLEE010000000
VERSION     DATLEE010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7590761"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VM692_05110"
     CDS             <1..1000
                     /locus_tag="VM692_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51577.1"
                     /translation="SLSALADGIRDVGRAPIILLCVYVVTLFTALPFTALMRGSIASH
                     LGNSLAADEAARGFNYRWLIEYQSQAGELGRTLRTTVIGFAAVLDNLSALIDNETRPA
                     SIVWIGAAYLLLWLYLTGGIIDRYARNRPTRSHEFFTACGVYFVRFLRLAPLIAAAYF
                     VLFAYVHPFLFNGVYSTLTRDVTAERTAFFYRLALYALFGVMLIAVNVTFDYAKVRAV
                     VEDRRSMIGAVLAGVRFIRRNLPTVVSLYVINGLMVLVVLFVYAVVAPGIGGTAPRLW
                     LGFLVSQIYLLGRLWVRLVFFASETSLFQATLSYVGYVASPAAPIPEPPIVEKLAG"
     gene            1159..2088
                     /locus_tag="VM692_05115"
     CDS             1159..2088
                     /locus_tag="VM692_05115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUQ51578.1"
                     /translation="MSQKLTLLKGGEPLPAEVVATEAQLTGSVRYLCDYVTRMLKLSP
                     AQVELIRPGFETLDLAIGDKIEDWNQVFLHILEELRQKGVELEQREALLSRMRRDGRT
                     LLYRRELGEERLLTTLREEHRGNGFGIEMVDHDRFKEYNDLFGHSVGDLVLSTTGRLF
                     RMHREGDIGVRWGGDEYLRIVRNLVTETQLGLIAGRDTELVDSFQWDVLDERFKDRPP
                     RVSIGLVYCERPSREERTRMMQCDEKANLSPEQRFDTSVPVPPGSSFDLARKLVGLAD
                     EKMYEAKREFRDSPDPHIAQVNVRITDGVLVII"
BASE COUNT          383 a          634 c          619 g          469 t
ORIGIN      
        1 aagtttgtca gcgctcgctg acggtatcag ggatgtcggc cgggcgccga tcatccttct
       61 atgtgtctac gtagtgactc tcttcaccgc gttgccgttt accgcgctga tgcgcggctc
      121 aatcgcgagt catctcggca acagcttggc cgccgacgag gcggcacgcg gattcaatta
      181 ccggtggctc atcgagtatc agtcgcaagc cggcgaactc ggaaggacgc tgcgaacgac
      241 ggtcatcggc ttcgccgcgg ttctcgacaa cctgagcgcg ctcatcgaca acgagacgcg
      301 cccggcgtct atcgtctgga tcggcgccgc gtacctgctc ctttggctat acctcacggg
      361 cgggatcatc gatcgatacg cccgcaatcg cccgacgcgg tctcacgagt tcttcaccgc
      421 gtgtggcgtc tatttcgtgc gatttctccg gctcgcaccg ttgattgcgg cggcctactt
      481 cgtgctgttt gcctacgtcc atcctttcct ttttaacggc gtttattcga ccctcacccg
      541 ggacgtgacc gccgagcgca cggccttttt ctaccgtctc gcgctctatg ctctgttcgg
      601 agtgatgttg atcgccgtca acgtcacatt cgactacgcg aaggttcggg cggtcgttga
      661 ggaccgccgc agcatgatcg gggcggttct tgccggtgtc cggttcatca ggcgcaacct
      721 ccctaccgtc gtcagcttgt acgtcatcaa tggcctcatg gtcctcgtcg tccttttcgt
      781 ctatgcggtc gtcgcgccag gaatcggcgg aacagcgcct cgactgtggt taggtttttt
      841 ggtgagccag atctatttgc tcggccggct ctgggtgcga ctcgtgtttt ttgcctccga
      901 aacttcgttg tttcaggcca cgctctcgta cgtcggctac gtcgccagtc cggcggcccc
      961 catcccagag cctccgatcg tggaaaagct agcgggttaa ctggttcgcg tgtcagcccg
     1021 ccgacacggc caaccattga cgtttacggc aaataccgaa ttttcacgct gcggcgtttt
     1081 ccgtctccgc atttcaaagt tgaacgcagc aacgctgtgg tattgaactt gctctgtagt
     1141 ggcgtgcaga gcacacaagt gtcccagaaa ctgaccttgc tcaagggtgg ggagccactg
     1201 ccggctgagg ttgtcgcgac cgaggctcag ctgacgggct cagtccgtta cctttgtgac
     1261 tacgtcacac gaatgttgaa attgagtccc gcccaggtcg agctgatccg cccgggattc
     1321 gaaaccctcg acctggcgat cggcgacaaa atcgaggatt ggaatcaagt ttttcttcac
     1381 atcctcgaag agcttcgcca gaagggcgtg gagctcgaac agcgcgaagc gcttctcagc
     1441 cgaatgcgcc gcgacggacg cacgctgctc taccgccggg agctcggcga agagcggcta
     1501 ctcacgacgc tgcgcgagga gcatcgcggg aacggctttg gtatcgagat ggtcgatcac
     1561 gacaggttca aggaatataa cgacctgttt ggacattcag tgggtgactt ggtgctgagc
     1621 acgacgggcc ggcttttccg catgcaccgt gaaggcgata ttggcgtgcg ctggggtggc
     1681 gacgagtatc tgcggatcgt ccgaaatctg gtcacagaaa cgcagctggg acttatcgcc
     1741 ggacgcgaca ccgagctcgt cgacagcttt caatgggacg ttctggacga acgattcaaa
     1801 gatcgtcctc ctcgcgtgag catcgggctc gtctactgcg agcggccgtc gcgcgaagaa
     1861 cgcacgcgca tgatgcagtg cgacgaaaaa gccaacctgt ctccggaaca acgattcgat
     1921 acgtcggtgc cagtgccccc tggctcgtcg ttcgacctcg cccgcaagct cgtcgggctc
     1981 gccgacgaga agatgtacga ggcgaagcgc gagttccgcg acagccccga tcctcacatt
     2041 gctcaagtga acgtgcgtat aaccgacggc gtgcttgtca ttatctaatc gatagcgggc
     2101 ggctc
//
LOCUS       DATLEE010000244         5368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2516149, whole genome shotgun sequence.
ACCESSION   DATLEE010000244 DATLEE010000000
VERSION     DATLEE010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516149"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /gene="aspS"
                     /locus_tag="VM692_05120"
     CDS             complement(<1..794)
                     /gene="aspS"
                     /locus_tag="VM692_05120"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HUQ51579.1"
                     /translation="MRSHYCGHVTRADIGRTVTVAGWVHRRRDHGGVIFIDLRDREGL
                     LQVVFDPDRAEMFKEAERLRSEYVVSVEARVRARPEGTVNPNLASGEVEVLATKLTVL
                     AKSKTPPFHHDEPVSEELRLRYRYLDLRREPMLKNLRLRHAVTRALRKFMDEHGFVDV
                     ETPILTKTTPEGARDYLVPSRTQPGNFFALPQSPQLMKQLLMMSGLDRYYQIVKCFRD
                     EDLRADRQPEFTQLEIETSFMSEEDILGLMEELVRDVFRSVLQVELP"
     gene            complement(947..1351)
                     /locus_tag="VM692_05125"
     CDS             complement(947..1351)
                     /locus_tag="VM692_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HUQ51580.1"
                     /translation="MPIYEYRCRACGHHLEALQKMTEAPLRKCPECGKSQLKRLVSAS
                     QFRLKGSGWYETDFKNKNETKRNLVERGGAEPSESSSDKSTDTAAKAEAGKAVAEAKA
                     DSTPAKPEKPKAKPTATRSKSAAKSRAKTARR"
     gene            complement(1373..3964)
                     /gene="clpB"
                     /locus_tag="VM692_05130"
     CDS             complement(1373..3964)
                     /gene="clpB"
                     /locus_tag="VM692_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HUQ51581.1"
                     /translation="MDKLTTQFQQALADAQSLAVGRDHQFIEPVHVLASLLEQRGGSV
                     VGILTKAGVNLNLMRSQLGDALDRVPSVEGTPGEVHIGNELSKLLNVTDKLAQERGDS
                     YISSELFLLAALQAKGDLGRILEKAGGVKGAIAKAIDEVRGGGKVDDQGAEEKRQALE
                     KYTIDLTARAEQQKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGQVPEGLKSKRVLSLDMGSLIAGAKFRGEFEERLKAVLNDLAKQEGQVILFID
                     ELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRKYVEKDAALERRFQKV
                     MVDEPSVEDTIAILRGLKERYAVHHKVEITDPAIVAAATLSHRYISDRQLPDKAIDLI
                     DEAASRIRMEMDSKPEEMDRLERRLIQLKIEREALKKEQDDASKRRLADLEEDIARFE
                     AEYADLEEIWKAEKAVVQSEADIKEELERMRDELEKARRASDLGRMAELQYGKIPELE
                     RRLEQALKAEREGSNKLLRNKVTDEEVAEVVSKWTGIPVAKMLEGEKDKLLRMEQHLE
                     ARVVGQTEAVQTVSNAIRRARAGLSDPNRPNGSFLFLGPTGVGKTELTKALASFLFDT
                     EEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVVLLDEVEKAH
                     PDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIVMTSNLGSQRIQELAGAANYERMKAEV
                     MEIVGQHFRPEFINRIDDVVVFHPLEAEHIRKIVDIQLEYLRRRLHEREIGLVLDAAA
                     IDRLAQAGFDPVYGARPLKRAIQQQLENPLAQRILRGDFAPGDTIRVTADGGTIGFAK
                     GVKEQAA"
     gene            complement(4059..4790)
                     /gene="pgeF"
                     /locus_tag="VM692_05135"
     CDS             complement(4059..4790)
                     /gene="pgeF"
                     /locus_tag="VM692_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HUQ51582.1"
                     /translation="MSTTLSGFAPDWPAPPNVRAWVTERGGATRYGTLNLALHVGDDA
                     SSVAANRRRLRMALELPAEPSWLEQVHGTRVLDLERDAVVPADAAVTSGSAKVCTVMT
                     ADCLPVLLCDRAGTRVGVAHAGWRGLLDGVLPAAVSAMRVPAAQLLAWLGPAISQTAY
                     EVGAEVRDAFLARAPTAEKRFAANARGRWQADLYGLARDALTAAGVANVHGGGFCTFT
                     QADRFFSHRREAPCGRMATLIWLRG"
     gene            complement(4787..>5368)
                     /locus_tag="VM692_05140"
     CDS             complement(4787..>5368)
                     /locus_tag="VM692_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HUQ51583.1"
                     /translation="LPRAGLVHRLDKDTSGLLLVARTLTSQTLLSAALERREIKRTYR
                     AICQGVLTGGGSIDAPIGRHRRERTKMAVTENGRAARTHYRVVERFRAHTYCELELET
                     GRTHQIRVHMAHIRAPLLGDPVYGGRPKLPPNPSDELRAALQGFRRQALHASRLRLTH
                     PVTGAAFEIESPLAPDIAALLDFLRADAAGGAA"
BASE COUNT          798 a         1813 c         1763 g          994 t
ORIGIN      
        1 ggcagctcga cttgcagcac ggacctgaac acgtcacgca cgagctcctc catgaggccg
       61 aggatgtcct cctcgctcat gaagctggtc tcgatctcga gctgcgtgaa ctcgggctgc
      121 cgatccgcgc gcaggtcttc gtcgcggaag cacttgacga tctgatagta gcgatcgagg
      181 cccgacatca tcagcagctg cttcatcagc tgcggcgact gcggcagcgc gaaaaagttg
      241 cccggctgcg tgcggctcgg cacgagatag tcgcgcgcgc cctcgggcgt cgtcttcgtc
      301 agaatcggcg tctcgacgtc gacgaagccg tgctcgtcca tgaacttgcg cagcgcccgc
      361 gtcacggcgt ggcgcagccg caagttcttc aacatcggct cgcggcgcag atcgagatag
      421 cgatagcgta agcggagctc ctcgctgacg ggctcgtcat gatggaatgg cggcgtcttc
      481 gacttcgcga gcaccgtgag cttcgtcgcg agcacttcga cttcgccgct cgcgaggttc
      541 gggttcacgg tgccctcggg gcgcgcacgc acacgagcct cgacgctgac gacgtactcg
      601 gagcgcagcc gctcggcttc cttgaacatc tcggcgcggt ccggatcgaa gacgacttgc
      661 agcaagcctt cgcgatcgcg cagatcgatg aagatgacgc caccgtgatc gcggcgccgg
      721 tgtacccaac cggcaaccgt gaccgtccgg ccgatgtcgg cgcgggttac gtggccgcag
      781 taatgactgc gcatcgctcc agtccttttg agcctaacct tttgaatgca aaggtggcgt
      841 tagcctcgaa gaggccggga tgttacgggg aaggcgtatg gcgcgcaagc gcggccaacg
      901 tgccgtcgtt accgtttcgg ttcaaccgcc ggcactcgcg cgcgcgtcaa cggcgcgcgg
      961 ttttcgcgcg cgactttgct gcggatttgg agcgcgtggc ggttggtttc gctttcggtt
     1021 tctcgggctt cgcgggcgtc gagtcggcct tcgcttccgc gacggccttg ccggcttccg
     1081 ccttcgccgc cgtgtcggtc gacttgtcgc tcgaagattc gctcggctcg gctccgcctc
     1141 tctcgacgag atttcgtttc gtttcgttct tgttcttgaa gtcggtctcg taccagccgc
     1201 tacccttcaa gcggaactgc gacgccgaga cgagccgctt cagctgcgac ttgccgcact
     1261 ccgggcactt gcgcagcggc gcctcggtca tcttctgcaa ggcttcgagg tggtgcccgc
     1321 aggcacggca gcgatactcg tagatcggca tagagttaag gtctcagagt cctcaagcgg
     1381 cctgctcctt cacgcccttc gcgaagccga tggtgccgcc atcggccgtc actcggatcg
     1441 tgtcgccagg ggcgaaatcg ccgcgcagga tacgctgcgc gagcggattc tccaattgct
     1501 gctggattgc gcgcttcagc ggccgagcgc cgtagacggg gtcgaagccc gcttgcgcga
     1561 gccgatcgat cgccgcagcg tcgagcacga gcccgatctc acgctcgtgc aagcgtcggc
     1621 gcaggtactc gagctggatg tcgacgatct tgcggatgtg ctccgcctcg agcgggtgga
     1681 acacgaccac gtcgtcgatg cggttgatga actcgggccg gaaatgctgg ccgacgattt
     1741 ccatgacctc ggctttcata cgctcgtagt tcgcggcgcc cgcgagctct tggatgcgct
     1801 ggctgcccaa gttcgacgtc atgacgatga cggtgttgcg gaagtcgacg gtgcggccct
     1861 ggccgtccgt gagccggccg tcgtcgagca cctgcagcag cacgttgaac acgtcggggt
     1921 gcgctttctc gacctcgtcg agcagcacga ccgagtacgg tttacggcgc acggcctccg
     1981 tgagatagcc gccctcttcg tagccgacgt agcccggcgg cgcgccgatc agccgcgcca
     2041 cggagtgctt ctccatgaat tccgacatgt cgatgcggac catggcttct tcggtgtcga
     2101 acaggaatga cgcgagcgct ttcgtgagct cggtcttgcc gacgccggtc gggcccagga
     2161 atagaaacga gccgttcggc cggttcgggt cggacaagcc cgcgcgcgcg cggcggatcg
     2221 cgttcgatac cgtttgcacg gcctcggtct ggccgacgac gcgcgcctcg agatgctgct
     2281 ccatgcgcag cagcttgtcc ttctcgcctt cgagcatctt cgcgacggga atgccggtcc
     2341 atttcgagac gacctcggcg acttcctcgt cggtgacttt gttgcgcagc agtttgttgc
     2401 tgccctcgcg ctcggccttc agtgcctgct cgagccggcg ctcgagctcg gggatcttgc
     2461 cgtactgcag ctcggccatg cggcccaagt cggacgcgcg gcgggccttc tcgagctcgt
     2521 cgcgcatgcg ctcgagctcc tctttgatgt ccgcttcgct ctgcacgacg gccttctcgg
     2581 ccttccagat ctcctcgagg tcggcgtact cggcctcgaa gcgagcgatg tcctcctcga
     2641 ggtctgcgag gcggcgcttc gacgcgtcgt cctgctcttt cttcagcgcc tcgcgctcga
     2701 tcttgagctg gatcaaacgg cgctcgagcc gatccatctc ctcgggcttc gagtccatct
     2761 ccatgcggat ccgcgacgcg gcctcgtcga tgaggtcgat ggccttgtcg ggcaactgcc
     2821 gatcgctgat gtagcgatgc gagagcgtgg ccgccgcgac gatcgcgggg tccgtgatct
     2881 cgaccttgtg atggacggcg taacgttcct tcaagccgcg caggatcgcg atcgtgtcct
     2941 ctacggacgg ctcgtcgacc atgacctttt gaaaccgccg ctcgagcgcc gcgtccttct
     3001 cgacatactt gcgatactcg tcgagcgtcg tcgcgccgac gcagtgcagc tcgccgcgcg
     3061 cgagtgccgg cttcagcatg ttgccggcgt ccatcgcgcc ctcggctttg ccggcgccga
     3121 ccatcgtgtg cagctcgtcg atgaacagaa tgacctgacc ttcctgcttc gcgagatcgt
     3181 tcagcacggc cttcagccgc tcctcgaact cgccgcgaaa tttggcaccg gcaatcaacg
     3241 agcccatgtc gagcgacagc acgcgcttcg atttcaaacc ttccggcact tggccgttga
     3301 cgatgcgctg cgcgagccct tcgacgatcg ccgtcttgcc gacgccgggc tcgccgatca
     3361 gcacgggatt gttcttcgtg cgccgctgca gtacttggat cgtacggcgg atctcgtcgt
     3421 cgcggccgat gacggggtcg agcttctgct gctcggctcg cgccgtcaga tcgatcgtgt
     3481 acttctcgag cgcttggcgc ttctcttcgg cgccttgatc gtcgaccttg ccgccgccgc
     3541 ggacctcgtc gatggccttg gcgatcgcgc ctttgacgcc gccggccttc tcgaggatgc
     3601 ggcccaagtc acccttcgcc tgcaatgccg cgagcaagaa cagctcgctc gagatgtagc
     3661 tgtcgccgcg ttcctgcgcg agcttgtccg tgacgttcag cagcttgctg agctcgttgc
     3721 cgatgtgcac ctcgcccggc gtaccctcga cgctgggcac gcgatcgagc gcatcgccaa
     3781 gctgtgagcg catcaggttc aagttcacgc cggctttggt caggatgccg acgacgctgc
     3841 caccgcgctg ctcgagcagc gaggcgagca cgtgcacggg ctcgatgaac tgatggtcgc
     3901 gccccaccgc cagcgactgc gcgtcggcga gcgcctgttg gaattgggtc gtcagcttgt
     3961 ccatgcgcat aactaggcgc tccggtcgtc ttgtggcacc cgccagacat tagggccggc
     4021 gcggccgatc tcaaccgcga gacgccaaac ggcgcgcatc agccgcggag ccagatcagc
     4081 gtcgccatgc ggccgcaagg cgcctcgcgg cggtgcgaga agaaccggtc ggcttgcgtg
     4141 aacgtgcaga agccaccgcc gtgcacgttc gctaccccgg ccgccgtcag cgcgtcgcgc
     4201 gccaggccgt agagatcggc ctgccaccgg ccgcgagcat tggccgcgaa ccgcttttcc
     4261 gccgtcggag cacgcgcaag gaacgcatcg cgaacctcgg cgccgacctc gtaggccgtc
     4321 tggctgatgg cgggcccaag ccacgcaagc agctgggcgg cgggcacgcg catggcggag
     4381 accgcggctg gcagcacgcc gtcgagcaga ccgcgccagc cggcatgcgc gacgccgacc
     4441 cgcgtgccgg cgcggtcgca gagcagcacc ggaagacagt cggccgtcat gactgtgcag
     4501 accttggccg agccggacgt gacggcggcg tcggccggca cgacggcatc gcgctcgaga
     4561 tcgaggacgc gcgtcccatg gacttgctcg agccagctcg gctcggccgg cagctcgagc
     4621 gccatacgca gacgcctgcg gttggccgcg acggagctcg cgtcgtcgcc gacgtgcagc
     4681 gcgagattca acgtgccgta gcgcgttgcg ccgccgcgtt ccgtgaccca ggcgcgaacg
     4741 ttcggcggcg cgggccagtc cggcgcgaag cccgacagcg tcgtgctcac gcggcaccgc
     4801 ccgcggcgtc cgcgcgcaaa aagtcgagca gcgcggcgat gtccggcgcg agcgggctct
     4861 cgatctcgaa agcagctcca gtcaccggat gcgtgagccg caggcggctc gcatgcaatg
     4921 cctggcgacg aaacccttgc agggccgctc gcagctcgtc gctggggttc ggcggcagct
     4981 tcggccggcc gccgtagacg ggatcgccga gcagcggcgc gcggatgtgc gccatgtgca
     5041 cgcgaatctg atgcgtgcgc cccgtctcga gctcgagctc gcagtacgtg tgcgcgcgaa
     5101 atcgctcgac gacgcgatag tgcgtgcgcg ccgcgcggcc gttctcggtt actgccatct
     5161 tggtgcgctc gcggcggtgc cggccgatcg gcgcgtcgat gctgccgccg cccgtgagga
     5221 caccctgaca gatcgcccga taggtccgct tgatctcgcg gcgctcgagc gcggcactga
     5281 gcagcgtttg gctcgtcagc gtccgcgcga cgagcagcaa gccgctcgtg tccttgtcga
     5341 ggcggtggac gagaccggca cgcggcaa
//
LOCUS       DATLEE010000245         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3128074, whole genome shotgun sequence.
ACCESSION   DATLEE010000245 DATLEE010000000
VERSION     DATLEE010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3128074"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VM692_05145"
     CDS             <1..211
                     /locus_tag="VM692_05145"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HUQ51584.1"
                     /translation="IANRGRTIVYAPTIKRERVRKPLVKTFLRRFFGNDLRPLFAQLL
                     QDEKLSAEDLEYLRKLIAETEPAKK"
     gene            208..1257
                     /locus_tag="VM692_05150"
     CDS             208..1257
                     /locus_tag="VM692_05150"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HUQ51585.1"
                     /translation="MSALVETLATALALSGLAALCLAVLPQAPPRLKFAIAVAGLAAW
                     LVPWSSLRDALPAATLELPLIAAAAHAPQPIEARLDAGTLLAVTLAAATLIGLALFTR
                     DCLALRLCVRRWRATSRPADHLRALLPPELAALRAEIRVVAHSNVAAASGYLEPTIWI
                     GDRYAGEPLRLMVVHEMWHVRSRDPVWLALLAAVRRTYWWNPLVAHLARQATLMLESI
                     CDHRSAAALGKVRYRSELASLVLAGAAPAPRLMATVHTANLDVQRVRLLGATLRLRAR
                     DVVVMAAFGVGAAAASLTSVAAPEATHRARSSVPTASATPTSANASDAELLDDLLSSY
                     AYTPQQNLGNSDAAR"
     gene            1272..1688
                     /locus_tag="VM692_05155"
     CDS             1272..1688
                     /locus_tag="VM692_05155"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51586.1"
                     /translation="MALVRKVLGTTCVLAATLALAPAQAHHSYAAFDRCTPVELEGEI
                     ANVEWANPHIRISLRTAEVASYSVEWFSLIQMERAGLAAEVLKTGDHVVITGKAMRDP
                     SMKILSLVTEIRRTSDGWTWTRATPQQLPPDCATTN"
     gene            1751..>2051
                     /locus_tag="VM692_05160"
     CDS             1751..>2051
                     /locus_tag="VM692_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51587.1"
                     /translation="MGFRLAATALLLASTVVGGCATPPEREPALHTLKVGDRSDTLVV
                     MLPGMRDRAEQFLVAGFVDGDTTADDFDVVVVEPEFSDYMRGTIVDRLRTDVIGPA"
BASE COUNT          324 a          722 c          685 g          320 t
ORIGIN      
        1 catcgcgaac cgcggccgca cgatcgtcta cgcgccgacg atcaagcgcg agcgcgtccg
       61 caagccgctc gtcaaaacct tcctgcggcg tttcttcggc aacgatctac ggccgttgtt
      121 tgcgcagctg ctgcaagacg agaagctcag cgccgaagac ctcgagtatt tgcgcaagct
      181 gattgccgaa acggagccgg cgaagaagtg agcgcgctcg tcgaaacgct cgcgacggcg
      241 ctcgcgctga gcgggctcgc cgcgctctgc ctcgcggtgc taccgcaagc gccgccgcgc
      301 ctgaagttcg cgatcgccgt ggcgggtctc gccgcctggc tcgtgccgtg gagctcgctg
      361 cgcgacgcgc tgccagccgc cacgctcgag ttgccgctga tcgcggcagc cgcccacgcg
      421 ccgcagccga tcgaagcgcg gctcgacgcc ggcacactgc tcgccgtcac gcttgccgcc
      481 gcgacgctga tcgggctcgc cctgttcacg cgcgactgcc tggcactgcg cctttgcgtg
      541 cggcggtggc gtgcgacgag ccggccggcg gatcacttgc gcgcgctatt gccgcccgag
      601 ctcgccgcac tgcgagccga aatccgcgtc gtcgcgcata gcaacgtcgc ggcggcctcc
      661 ggctacctcg agccgacgat ctggatcggc gatcgctacg ccggcgagcc actgcggctg
      721 atggtcgtgc acgagatgtg gcacgtccgc agccgagacc ccgtctggct tgcgctgctc
      781 gcggccgtgc gacgcacgta ttggtggaat ccgctcgtcg cgcatctcgc gcgccaggcg
      841 actctgatgc tcgagtcgat ctgcgatcac cgcagtgccg cggcgttggg caaggtccgc
      901 tacagaagcg agctcgcgtc gctcgtgctc gccggcgctg cgccggcgcc gcgattgatg
      961 gcgacggtgc acacggccaa cctagacgtg cagcgtgtgc gcttgctcgg cgcgacgctg
     1021 cgtctgcggg cgcgcgacgt cgtcgtgatg gccgccttcg gcgtcggcgc cgcggcggcg
     1081 tcgctgacga gcgtcgcggc gcccgaggct acgcaccgcg cacgatcgtc cgtgccaacg
     1141 gcgtcagcga ctccgacgag cgcaaacgcc agcgacgccg agctgctcga cgacttgctg
     1201 agcagctacg cctacacgcc gcagcagaac ctcggcaact cggacgccgc gcgctaactg
     1261 ggagacgcct catggccctc gtccgcaagg tccttggtac aacctgcgtg ctagcggcga
     1321 cgctggcgct cgcaccggcg caagcgcatc acagctatgc ggccttcgat cgctgcacgc
     1381 ccgtcgagct cgaaggtgag atcgccaatg tcgagtgggc gaatccgcac atcaggatca
     1441 gcctgcgtac cgcggaggtc gccagctact ccgtcgagtg gttcagcctg attcaaatgg
     1501 agcgagccgg actcgcggcc gaagtcctga agaccggtga ccacgtcgtc atcacgggta
     1561 aagcgatgcg cgacccgagc atgaaaattc tttcgctcgt gaccgagatt cgccgtacga
     1621 gcgacgggtg gacgtggacg cgcgcgacgc cgcagcagct gccgcccgac tgcgccacta
     1681 cgaactgacg ttacagcctc cctggcatag gagttgcacc gcagctcgac cctgaacgag
     1741 gagcgggctc atgggatttc gcctcgccgc gacggctctg ctgctcgcaa gcacggtcgt
     1801 cggcggctgc gcgacgccgc ccgaacgcga gccggcacta cacacgctga aggtcggcga
     1861 ccgcagcgac acgctcgtgg tcatgctgcc cggaatgagg gaccgagcgg agcaatttct
     1921 cgttgccggc ttcgtcgacg gcgacacgac ggcggatgac ttcgacgtcg tcgtcgttga
     1981 gcccgagttc agcgactaca tgcgcggcac gatcgtcgat cgcctgcgga cggacgtcat
     2041 tggacccgcg c
//
LOCUS       DATLEE010000246         2727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3998299, whole genome shotgun sequence.
ACCESSION   DATLEE010000246 DATLEE010000000
VERSION     DATLEE010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3998299"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VM692_05165"
     CDS             <1..777
                     /locus_tag="VM692_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUQ51588.1"
                     /translation="NPEAWEWYYHVVGEARCPIVDTWWQTETGGMLITPFPGATKLKP
                     GSATLPFFGVKPAIVDQNGKILEGACSGNLVLLDSWPGQMRTVYGDHQRFVDTYFKQF
                     PGMYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVGHTKVAEAAVVGF
                     PHDLKGQGIYAYVTLKAGETPSDGLRKELVAWVRKEIGPIAQPDVIQWAPSLPKTRSG
                     KIMRRILRKIAANDFKELGDTSTLADPAVVDDLIKNRAAR"
     gene            complement(784..1701)
                     /locus_tag="VM692_05170"
     CDS             complement(784..1701)
                     /locus_tag="VM692_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HUQ51589.1"
                     /translation="MDNLEDIAVFVKVVDAGSFTGAAEALETSQPVVSKSVTRLEQRL
                     GVRLLNRTTRRISLTEAGTELYRKAASALTQLDEAQLDIGRYQSEPRGTLRLSAPTSF
                     TLLHLPHRFAQFLERHPQVTLDLVLEDRVVDIVEEGFDAAIRVGVLSSSTLVARRLAP
                     CKQVFCASPAYLERHGKPESVDDLLKHNCVVYTLGRDPRLWRVRAEDGTEVQLPVRGN
                     AYVNSGLLEQALALEGLGLAMLPTFYVGDALREGRLVRVLEHVVLPEIGIYLVYADRR
                     NLLPKLKALLDFLVESFGGEPDWERGLEL"
     gene            1812..2240
                     /locus_tag="VM692_05175"
     CDS             1812..2240
                     /locus_tag="VM692_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HUQ51590.1"
                     /translation="MKQFESSIAAGNLLRVSLGVMYLTHSLLLKVATYGFDGTAQYFE
                     SIGLPGALAYFTIVAEAAGGVLLIAGYKARETALLLVPILVGALWAHSGNGWVFTNAG
                     GGWEYPLFLIAVSVAVALNPGRQLAVGAARVSPQPATLVR"
     gene            complement(2297..>2727)
                     /locus_tag="VM692_05180"
     CDS             complement(2297..>2727)
                     /locus_tag="VM692_05180"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HUQ51591.1"
                     /translation="KVLLTVRDPGEWFDSTQTTIFSPLMRARVGDSPAAEFLEKTVWG
                     DFGDRLHDRDYMIAAFERHTAEVKSAIPRERLLVLDVKQGWKPLCDFLGVAIPSKPLP
                     KLNSREELGARITATREKGGEQDFLANAKRYVAELSKLSE"
BASE COUNT          473 a          916 c          893 g          445 t
ORIGIN      
        1 aaccccgagg cgtgggagtg gtattaccac gtcgtcggcg aagcgcgctg cccgatcgtc
       61 gacacgtggt ggcagacgga gacgggcggc atgctgatta cgccattccc gggcgcgacg
      121 aagctaaagc cgggctctgc gacgctgccg ttcttcggcg tgaagccggc catcgtggac
      181 caaaacggca agattcttga aggcgcgtgc tcgggcaacc tcgtgctgct cgatagctgg
      241 cccgggcaga tgcgcaccgt ctacggcgat caccagcgct tcgtcgacac ctatttcaag
      301 caattccccg gcatgtactt cacgggcgac ggcgcgcgcc gcgacgagga cggctattac
      361 tggatcacgg gccgcgtcga cgacgtcatc aacgtctcgg gacaccgctt gggcacggcc
      421 gaggtcgaga gcgcgctcgt cgggcacacg aaagtcgccg aggcggccgt cgtcggcttc
      481 ccgcacgact tgaaaggcca aggcatctac gcctacgtca cgctgaaggc cggcgagacg
      541 ccgagcgacg ggctgcgcaa ggagctcgtc gcctgggtgc gcaaggagat cggtccgatt
      601 gcgcaacccg acgtgatcca gtgggcgccg agcctgccga agacgcgctc gggcaagata
      661 atgcggcgca tcctgcgcaa gatcgcggcc aacgacttca aggagctcgg cgacacgtcg
      721 acgctcgcgg acccggccgt cgtcgacgac ctcatcaaga accgcgcggc acgctagcgg
      781 ccgtcacaac tcgagcccgc gctcccagtc gggctcgccg ccgaagctct cgacgaggaa
      841 gtcgagcagc gccttgagct tcggcagcag attgcgccga tcggcgtaga cgagatagat
      901 gccgatctcg ggaagcacga cgtgctcgag cacgcgcacg agtctgcctt cacgcagcgc
      961 gtcgccgaca taaaacgtcg gcagcatcgc gagacccaag ccttcgagcg cgagcgcctg
     1021 ctcgagcaga ccgctgttga cgtaggcgtt gccgcgcact ggcagctgca cttcggtgcc
     1081 gtcctcggcc cgcacgcgcc agagccgcgg gtcacgcccg agcgtgtaga cgacgcagtt
     1141 gtgcttcaaa aggtcgtcga cggattccgg tttgccatga cgttccaagt acgccggcga
     1201 agcgcagaac acttgcttgc agggcgcgag ccgccgggcc acgagcgtcg agctcgagag
     1261 cacgccgaca cggatcgccg cgtcgaagcc ttcctcgacg atgtcgacga cgcgatcctc
     1321 gagcacgaga tcgagcgtga cctgcggatg gcgctcgagg aactgcgcga agcggtgcgg
     1381 caggtgcagc aacgtgaacg acgtcggcgc gctgaggcgc aaagtgccgc gcggctcgct
     1441 ctgataacgg ccgatgtcga gctgcgcttc gtcgagctgc gtcagcgcgc tcgccgcctt
     1501 gcgataaagc tccgtgccgg cctccgtcag gctgatgcgc cgcgtcgtgc ggttcaacag
     1561 ccgcacgccg agacgctgct cgagccgcgt gacgctcttg ctgacgacgg gctgcgacgt
     1621 ctcgagcgct tcggcggcgc ccgtgaagct gcccgcatcc accactttga cgaacaccgc
     1681 gatgtcctcg aggttgtcca ttccgattga tgctcccccg gcatgaacgt tatgccgctg
     1741 gcccggatta tccctgcgac gagcggcgct aaggtaccgc ccgtcgaaac atcaactcat
     1801 gggagccagt catgaaacag ttcgaatctt cgattgcggc cggcaacttg ctgcgcgtca
     1861 gcctcggcgt catgtatctc acgcacagcc tgctgctcaa ggtagccacg tacggcttcg
     1921 acggcacggc gcaatacttc gagtcgatcg ggctgcccgg cgcgctcgcg tacttcacga
     1981 tcgtcgccga agccgccggc ggcgtgctgc tgatcgccgg ctataaggcg cgggaaaccg
     2041 ccttgctgct cgtgccgatc ctcgtcggcg cgctctgggc gcactccggc aacggttggg
     2101 tcttcaccaa cgccggcggc ggttgggaat acccgttgtt cctgatcgcc gtatcggtcg
     2161 ccgtcgcttt gaacccgggt cggcagctcg cggtcggcgc cgcgcgggtt agcccgcagc
     2221 ccgcgacgct cgtacggtga tacgacggtg acaacccgac gcggcgaagc acaatccgtg
     2281 cacgccgcgg cgggtgttac tccgagagct tgctcagctc cgcgacgtag cgcttcgcgt
     2341 tcgcgaggaa atcttgctcg ccgcccttct cgcgcgtcgc cgtgatcctg gcgccgagct
     2401 cctcgcgcga gttgagcttc ggcagaggct tgctcggaat cgccacgccc aagaaatcgc
     2461 agagcggctt ccagccctgc ttgacgtcga gcacgagcaa ccgctcgcgc ggaattgcac
     2521 tcttcacttc ggccgtgtgc cgctcgaaag ccgcgatcat gtagtcgcgg tcatggaggc
     2581 ggtcgccgaa gtcgccccag acggtcttct cgagaaactc cgcggccggc gagtcgccga
     2641 cgcgagcgcg catcagcggc gagaagatcg tggtctgcgt cgagtcgaac cactcgccgg
     2701 ggtcgcgcac cgtcagcagc actttgg
//
LOCUS       DATLEE010000247         2865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8336832, whole genome shotgun sequence.
ACCESSION   DATLEE010000247 DATLEE010000000
VERSION     DATLEE010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8336832"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1422
                     /gene="trpB"
                     /locus_tag="VM692_05185"
     CDS             175..1422
                     /gene="trpB"
                     /locus_tag="VM692_05185"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HUQ51592.1"
                     /translation="METKEREALLAALLEGRLPDKNGRFGPFGGRYVPETLMPAIERL
                     MHGVEHILPSAEFQAELTRELKGWIGRPTALTHTPTLSKRWGADVWLKREDLAHTGAH
                     KINNGIGQALLAKALGATRVVAETGAGQHGVASAAACARVGLPCTVYMGEIDVARQAP
                     NVDRMRRFGATVVPVTSGDRTLRAAIDEAIRDWVSDPVGTYYLLGSAIGPHPYPYLVR
                     QLQAVIGREARAQMQSEAGGLPDAVFACVGGGSNAIGIFHGFLADRAVNIFGVEAGGR
                     GPALGDHSATLSHGKPGVLHGTHTMLLYDQHGQIQETHSISAGLDYPGVGPEHALLQA
                     IGRVEYVTASDDETLAALEELTATEGILPALESSHALAGAKRWAARNPGARVLVGLSG
                     RGDKDMPILAKYPMGRERQHAAA"
     gene            1409..2182
                     /gene="trpA"
                     /locus_tag="VM692_05190"
     CDS             1409..2182
                     /gene="trpA"
                     /locus_tag="VM692_05190"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HUQ51593.1"
                     /translation="MQPREHVRAALARDVTKKTRPSLVAFVTAGYPEPRDFLKVLRSV
                     ASAADAVEIGVPFSDPMADGVTIQRSSQHAIEKGVSLLWILDELARRDFELPSPVLLM
                     SYLNPLLAFGYERLAKRAVEVGVSGFIVPDLPYEESAPLAAALEPHGLALVQLVTPAT
                     PAERLRRLCAASQGFVYAVTRTGITGGESTLPAETTQYLAAVKAASPLPVCAGFGVRR
                     ADQVALRGAHADGVIVGSALVEVLERGADPAVFLRSLRL"
     gene            2202..2528
                     /locus_tag="VM692_05195"
     CDS             2202..2528
                     /locus_tag="VM692_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HUQ51594.1"
                     /translation="MKRVLSGNSLPDTAHFKNLLEQSGIPCLVKNRELGGGLGDLPVF
                     DCAPELWVLDDARASQAEALIRDSLRATARGTPWRCSSCGEDNEPQFGACWACGSADP
                     RAGESV"
     gene            2530..>2865
                     /locus_tag="VM692_05200"
     CDS             2530..>2865
                     /locus_tag="VM692_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51595.1"
                     /translation="MRWVDRLLMLCAAIVVAANLLPLGARWSWMLELTTHFRVQYLAV
                     TAALLMLVALRRKWRAAAALVLAGAVSALPVLPYIPLSVTPAAAAGPAAIKVLTVNVS
                     YRQFAVRRLL"
BASE COUNT          425 a          965 c         1025 g          450 t
ORIGIN      
        1 cgagcgcgcg cgcggggaga aggaccccga gaagatcaaa cagttcgtcg cccgcgtacg
       61 ggcgatcgac gcgggcacgg atgtccgcgg cagaacgccg catgcgaggg cagggatgcc
      121 cgaggtagag caacgcaggg agcaattgcc gggaacggat gtcgaggtat gacgatggaa
      181 acgaaggaac gcgaggcact gctcgcggca ctgctcgaag ggcgattgcc ggacaagaac
      241 ggccggttcg ggccgttcgg cggccgctac gtgcccgaga cgctgatgcc tgctatcgag
      301 cgactcatgc acggcgtcga gcacatcttg ccgtcggcag agttccaagc cgagctgacg
      361 cgcgagctca aaggttggat cggccggccg acggcgctga cgcacacgcc gaccttgagc
      421 aagcgctggg gcgcggacgt ttggttgaag cgcgaagacc tcgcgcacac gggcgcgcac
      481 aagatcaaca acggcatcgg ccaggcgctg ctcgcgaagg cgttgggcgc gacgcgcgtc
      541 gtcgccgaaa ccggcgccgg ccagcatggc gtcgcgtcgg ccgcggcctg cgcgcgcgtc
      601 ggcctaccgt gcacggtcta catgggcgag atcgacgtcg cgcgccaagc gccgaacgtc
      661 gaccgcatgc gccgcttcgg cgccaccgtc gtccccgtga cgtcgggcga ccgcactctg
      721 cgcgccgcga tcgacgaggc gattcgcgac tgggtctcgg atcccgtcgg cacttactat
      781 ttgctcggct ccgccatcgg cccgcatccg tatccgtatc tcgtgcgcca gctgcaggcc
      841 gtgatcggcc gcgaagcgcg cgcgcagatg cagagcgagg cgggcgggct gccggatgcc
      901 gtgttcgcgt gcgtcggcgg cggctcgaat gccatcggca tctttcatgg cttcctggcc
      961 gaccgcgccg tcaacatctt cggcgtcgaa gcgggcggcc gcggacccgc gctcggcgat
     1021 cactcggcga cgctgtcgca cggcaagccc ggcgtgctgc acggcacgca cacgatgctg
     1081 ctgtacgacc agcacggcca gatccaggag acgcactcga tctcggcggg gctcgactac
     1141 cccggcgtcg gccccgagca cgccttgctg caagcgatcg gccgcgtcga gtacgtgacg
     1201 gccagcgacg acgaaacgct cgcggcgctc gaggagctga cggcgaccga aggcatcctg
     1261 ccggcgctcg agagctcgca tgcgttggcc ggcgccaagc gttgggccgc ccggaacccc
     1321 ggcgcgcggg tactggtcgg actctcgggc cgcggtgaca aggacatgcc gattctcgcc
     1381 aaatacccga tgggtcggga gcggcagcat gcagccgcgt gagcacgttc gcgcagcgtt
     1441 ggcgcgcgac gtgacgaaga aaacgcgccc gtcgctggtc gcgttcgtca cggcgggcta
     1501 ccccgagccg cgcgacttct tgaaggtctt gcgctcggtc gcgagcgccg ccgatgccgt
     1561 cgagatcggc gtgccgtttt cggacccgat ggccgacggc gtgacgatcc agcgctcgag
     1621 tcagcacgcg atcgaaaaag gcgtgagcct cctctggatc ttggacgagc tggcccggcg
     1681 cgacttcgag cttccgtcgc ccgtgctgct gatgagctat ctgaacccgc tgctcgcgtt
     1741 cggctacgag cggctcgcga agcgcgcagt cgaagtcggc gtcagcggct tcatcgtgcc
     1801 ggatctgcca tacgaggaaa gcgcgccgct cgccgcggcg ctcgagccgc acgggttggc
     1861 gctcgtgcag ctcgtgaccc cggcgacgcc tgccgagcgc ttgcgtaggc tgtgtgcggc
     1921 gagccaaggt ttcgtctatg ccgtcacgcg gacgggcatc acgggcggcg agtcgacgct
     1981 gcccgcggag acgacgcagt acctcgcggc cgtcaaagcc gcgtcgccgt tacccgtgtg
     2041 cgccggcttc ggcgtgcggc gcgcggacca ggtagcctta cgcggcgcgc acgccgacgg
     2101 cgtcatcgtc gggtcggcgc tggtcgaagt cctcgagcgc ggcgccgatc ccgcggtttt
     2161 cttgcgttcg ttgcgtctgt gacgtcatgg agccgccggc catgaagcga gtgttgagcg
     2221 gcaactcgtt gcccgacacg gcccatttca agaacctgct cgagcagtcg ggcattccgt
     2281 gcctcgtcaa gaatcgcgag ctcggcgggg gcctcggcga cttgccggtg ttcgattgcg
     2341 cgcccgagct ctgggtgctc gacgacgcac gcgcgagtca agccgaagcg ctgattcgcg
     2401 acagccttcg ggcgacggcg cgcggcacac cgtggcgctg cagctcgtgc ggcgaggaca
     2461 acgagccgca attcggcgct tgctgggctt gcggcagcgc ggatcctcgc gccggggagt
     2521 cggtctagca tgcgttgggt cgatcggctg ttgatgctct gcgcggccat cgtcgtggcg
     2581 gcgaatctgt tgccgctcgg cgcgcggtgg tcatggatgc tcgagctcac gacgcacttc
     2641 cgcgtgcaat acctcgccgt caccgccgcc ctgctcatgc tcgtggcgct gcggcggaaa
     2701 tggcgtgccg cagcggctct cgtgctggcg ggcgcggtca gcgcgttgcc cgtgctgcct
     2761 tacattccgc tctctgtcac gccggcggcg gctgccgggc cggcggcgat caaggtgctg
     2821 accgtcaacg tctcgtatcg gcagttcgcc gtgcgccggc tgctc
//
LOCUS       DATLEE010000248         4317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2518130, whole genome shotgun sequence.
ACCESSION   DATLEE010000248 DATLEE010000000
VERSION     DATLEE010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2518130"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..824
                     /locus_tag="VM692_05205"
     CDS             159..824
                     /locus_tag="VM692_05205"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51596.1"
                     /translation="MKLSYVALVASMLLAAPALAQEQNRPAGPGPGQPPRGQPPAATA
                     PAVEAPVPPPLSPAQLESQRRRDEQSVDLDQLLARVSQKTGKQFLVDPRVRVRVYGVP
                     KIEDPSYEELLAILRLHGYAAAEIGGRVNIVPDANARVLPVRLLNRDDDSVPDDEFVT
                     RVIEVRNAHLLVPVLRPLMPQSAHLAAVINEQDASAPGKLILMDTYANIRRMTELINT
                     LAR"
     gene            complement(1094..2707)
                     /locus_tag="VM692_05210"
     CDS             complement(1094..2707)
                     /locus_tag="VM692_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HUQ51597.1"
                     /translation="MNCASATARAGLLLLAGLWLAAPASAALEEPLELDAGLLGKSTE
                     SSPGIRAFKGIPFAAPPVGALRWKEPQPVPKWSGVRDASKFGNVCIQPAGPTTGPGAR
                     LNIAVLPDSPPLSEDCLYLNVWTGAASAGERRPVMVYIFGGAFTEGAGSVPLYDGDAL
                     AKKGAVVVTMNYRLGPYGFFAHPALTAESPHRASGNYGLMDMLASLRWVQSNIAAFGG
                     DPGNVTVFGQSAGAMAIGSLVASPEAKGLMRRAISQSGSWLGLGPVPGMRTRAQAEQA
                     GVESATAAGVTTLAQLRAMSAADVTDKFRSAGMIVDGWVIPEDPATTFAAGRQNSVDV
                     LVGSNKDEAFFPGARTVKEFEEQARARWGTLAEQYLALYSHATDEEAQASAAQTSNDG
                     TFWTMRQYADYQARHGNEAYLYFFAQNPPAPAGQPAFPAAHAAEVPYVFNNLGKLPLF
                     PDRSDPKLAAASAPDLEVADQMSSYWVSFARTGNPNGPGLPMWQEHQAGGSDRAIILD
                     AEPASEKLPTKARLELHDKLYAQMRRGAQ"
     gene            complement(2704..3756)
                     /locus_tag="VM692_05215"
     CDS             complement(2704..3756)
                     /locus_tag="VM692_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51598.1"
                     /translation="MSDRVLSSIVLAAAVALAGCSDSASTGNAGTRAAGTADASGLPR
                     TAWGDPDLQGMWPIINLISTPFQRPVDEKGNPIYGDREELTDEEYTAAQQRLAARDQR
                     YQDEIKSNKMGMGHWAEASHSGDAARLTSLLVEPKNGRFPALTERGAELAAKMSSSWS
                     GKQFDKPTDFDTWDRCITRGLPSSMFPFNYNNGIEIIQAPGYVVLRLEMIHEARVIPI
                     DGRPPLDPAIKQWMGESRGHFESNTLVVETTNFNGIGGMTNVGVPGSPTGDTPTTTNM
                     KITERFTLTDRDTIQYEIYVDDPEVLTQPWKARYPMQRDDSYQFFEYACHEDNTAVRN
                     FIVTSRYERAQAAKAQ"
     gene            3958..>4317
                     /locus_tag="VM692_05220"
     CDS             3958..>4317
                     /locus_tag="VM692_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51599.1"
                     /translation="MRSAYKTINAASSFTLAIALAACGQSEQAQPAATVAAPTPTPTS
                     VAPGDWPLINRDLSASRFSPLTEITASNVASLTTSWTHPLGGNSTAVPIVVAGVMYMP
                     SRDRVVALDGDTGALIWE"
BASE COUNT          697 a         1476 c         1415 g          729 t
ORIGIN      
        1 gctcatcaag aatcgcgacg tcgcgccgaa gtaaaccggc gctgacggtc gcacctcccg
       61 cctgccctgg ctgcacacgc ccgcgcagca gggatggcgt ggcggaatct cctgtctcga
      121 acgtattaat cgagacccca acgagtcagg agaacccaat gaagctctcg tacgtggccc
      181 tcgtcgcgtc catgctgctg gcggccccgg cgctcgccca ggagcagaac cgccctgccg
      241 gcccaggccc ggggcaaccg ccgcgcggcc aaccgcccgc ggcgacggct cccgctgtcg
      301 aggcgcccgt gccgccaccc ctgtcaccgg cgcagctcga atcccagcgc cgccgcgacg
      361 agcaaagcgt cgacctcgat cagctgctgg cgcgtgtctc gcagaagacc ggcaagcaat
      421 ttctcgtcga cccgcgcgtg cgagtgcgcg tgtacggcgt gccgaagatc gaagacccga
      481 gctacgagga actgttggcg atcctgcggt tacacggcta cgcggcggcc gagatcggcg
      541 ggcgcgtcaa catcgtgccg gacgccaacg cgcgtgtttt gcccgtgcgt ctgttgaatc
      601 gcgacgacga cagcgtaccg gacgacgagt tcgtgacgcg cgtgatcgag gtccgcaatg
      661 ctcatctgct cgtgcccgtg ctaagaccct tgatgccgca atcggcgcac ttggcagccg
      721 tcatcaacga gcaagatgcg agcgcgcctg gcaagctcat cctgatggac acgtacgcga
      781 acatccgccg catgacggag ctgatcaaca cgctcgcgcg gtagtgcacg cggctgcgcc
      841 ggatgagcgg cacgcgtcgc ccgacgagcg gcgcggcgcc gacggacggt agcgccggcc
      901 cgacagttga cgggagcgcg gcagcgttcc agccatagac ttgctgccag cggcagctcg
      961 ttaacgagct gcgaacggag aacggccatg gcagggactt tgcctcacac gcagaatggc
     1021 ttcgtgcaga ccgtcgccga gcagcatttt tgcgagcaac tcgccgacga caacgccgcc
     1081 agtcgatctc gtcttactgc gcgccgcggc gcatttgcgc gtacagcttg tcgtggagct
     1141 cgaggcgcgc cttcgtcggc agcttctcgc tggccggctc cgcgtccaaa atgatcgcgc
     1201 ggtcactgcc gccggcttgg tgctcctgcc acatcggcag ccccgggccg ttcggattgc
     1261 cggtcctcgc gaagctcacc cagtacgacg acatctggtc cgcgacctcg aggtcgggcg
     1321 ccgatgcggc cgcgagcttc gggtcgctgc gatccggaaa caacggtagc ttgccgaggt
     1381 tattgaacac gtacggcact tcggcggcgt gcgccgccgg aaaagccggc tgaccggcag
     1441 gcgcgggcgg attctgcgcg aagaagtaca gatacgcctc gttgccgtgt cgcgcctggt
     1501 agtcggcgta ctgccgcatc gtccagaacg tgccgtcgtt cgacgtctgc gcggccgagg
     1561 cctgcgcttc ttcgtccgtc gcgtggctgt acagcgcgag gtactgctcg gcgagcgtgc
     1621 cccagcgggc gcgcgcctgt tcctcgaact cctttacggt acgcgcgccg ggaaagaacg
     1681 cttcatcctt gttcgagccg acgagcacgt caacggagtt ctggcgtccc gccgcgaacg
     1741 tcgtcgcggg atcctcgggt atgacccagc cgtcgacgat catgccggcg ctgcggaatt
     1801 tgtccgtgac gtcggcggcc gacatcgcgc gcagttgcgc gagcgtcgtg acgccggcgg
     1861 ccgtcgcaga ttcgactccg gcctgctccg cttgcgcgcg ggtacgcatg cccggcacgg
     1921 ggcccaaccc gagccacgag ccgctctgcg agatcgcgcg ccgcatgagc cccttcgcct
     1981 cgggcgacgc gacgagcgag ccgatcgcca tcgcgccggc cgactggccg aacacggtga
     2041 cattccccgg gtcgccgccg aacgccgcga tgttgctctg cacccaacgc agcgacgcga
     2101 gcatgtccat gagcccgtag ttgcccgatg ctcggtgcgg cgattccgcc gtcagcgccg
     2161 ggtgcgcgaa gaagccgtac ggccccaagc gataattcat cgtgacgacg acggcgcctt
     2221 tcttcgcgag cgcgtcgccg tcatagagcg ggaccgagcc cgcgccttcc gtgaaggcac
     2281 cgccgaaaat gtagaccatg acgggccggc gctcgccggc actcgccgca ccggtccaga
     2341 cgttcaaata caggcagtcc tcgctgagcg gcggcgaatc cggcagcacg gcgatgttga
     2401 gccgagcgcc ggggcccgtc gtcggcccgg ccggttggat gcaaacgttg ccgaacttgc
     2461 tcgcgtcgcg gacgccggac cacttcggga cgggctgcgg ctccttccag cgcagtgcgc
     2521 cgacgggcgg cgccgcgaac ggaatgcctt tgaaggcgcg aatgcccggc gacgattccg
     2581 tgctcttgcc gagcagaccg gcgtcgagct cgagcggctc ctcgagcgcc gcgctcgcgg
     2641 gcgccgcgag ccagagtccc gcgagcagca gaagaccggc tcgtgccgtg gcgctcgcgc
     2701 agttcattgc gctttcgcgg cttgggcgcg ctcgtagcgt gaggtgacga tgaaattgcg
     2761 cacggctgtg ttgtcctcgt gacaggcgta ctcgaagaac tgataactgt cgtcccgctg
     2821 cattggataa cgcgccttcc acggctgcgt cagcacctcc ggatcgtcga cgtagatctc
     2881 gtactgaatc gtgtcgcgat ccgtcagcgt gaaccgctcg gtaatcttca tgttcgtcgt
     2941 cgtcggcgtg tcgcccgtcg gcgagccggg aacgccgacg ttcgtcatgc cgccgatgcc
     3001 gttgaagttc gtcgtctcga cgacgagcgt gttgctctcg aagtggccgc gcgattcgcc
     3061 catccattgc ttgatcgcgg gatcgagcgg cgggcgcccg tcgatcggaa tgacgcgcgc
     3121 ctcgtggatc atctcgagcc gcagcacgac gtaacccgga gcctgaatga tctcgatgcc
     3181 gttgttgtag ttgaacggaa acatcgacga cgggaggcca cgcgtgatgc agcgatccca
     3241 ggtgtcgaaa tccgtgggct tgtcgaactg cttgccgctc caactgctgc tcatcttcgc
     3301 cgcaagctct gcgccgcgtt ccgtcagcgc cgggaaacgg ccgttcttcg gctcgacgag
     3361 cagcgaagtc agacgcgccg cgtcgccgct gtggctcgcc tcggcccagt ggcccatgcc
     3421 catcttgttg ctcttgatct cgtcctgata gcgctggtcg cgggccgcga gccgctgctg
     3481 cgccgccgtg tattcctcgt cagtcagctc ttcgcgatcg ccgtagatcg gattgccctt
     3541 ctcgtcgacg gggcgctgaa atggagtgct gatcagattg atgatcggcc acattccttg
     3601 gaggtcgggg tcgccccacg ccgtgcgcgg caagccggaa gcgtcggccg tgccggccgc
     3661 acgagtgccc gcgttgcccg tggacgcgga gtcgctgcag ccggcaagcg ccactgcagc
     3721 ggcgagaaca atcgagctca gcacacggtc gctcatcgca acctccacta gcctgcgagg
     3781 cccattcatc agcctgggcg cgacgcgggt tacgtgcaac caaagttttt ttaggtgcgc
     3841 ccgctgccgc ggcgcacgct ttctccctgg cgcaatcgag tatacggccg ccatcgagcg
     3901 gccggcacgg tgtatcgtac gcagccgaac tctcggggtg agaactaggg gcactctatg
     3961 cgatctgcat acaaaaccat caacgcagcg tcgtcattca cgttggcaat cgcgctcgcg
     4021 gcctgcggcc aatcggagca agcgcaaccg gcggcaacgg tcgccgcacc gacgcccact
     4081 ccgacgagcg tcgcgcccgg cgactggccg ctgatcaatc gcgatttgag cgcgagccgc
     4141 ttttcgccgt tgacggagat cacggcttcg aacgtcgcgt cgctgaccac gagctggacg
     4201 cacccgctcg gcggcaactc gacggccgtg ccgatcgtcg tcgccggcgt catgtatatg
     4261 ccgagccgcg atcgcgtcgt ggcgctcgac ggcgacaccg gcgcgttgat ctgggag
//
LOCUS       DATLEE010000249         2068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4003122, whole genome shotgun sequence.
ACCESSION   DATLEE010000249 DATLEE010000000
VERSION     DATLEE010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4003122"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..684)
                     /gene="hisH"
                     /locus_tag="VM692_05225"
     CDS             complement(82..684)
                     /gene="hisH"
                     /locus_tag="VM692_05225"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HUQ51600.1"
                     /translation="MKANAVIIPSCGSNLASLQFALERLGADVPLTEDPARIRAASHV
                     ILPGVGAAAPGMQRLAAAGLVELIPTLSQPVLGICLGMQLLFASSEEEQTRCLGVIDA
                     EVRRFPARADLPVPQMGWNEIEPVGDSPLLAGVAQGTYAYFVHSYAAPPGPYTRAVTS
                     YGVPFSAVIEQGNFYGTQFHPERSSRGGAQILDNFLKIGL"
     gene            complement(681..1739)
                     /gene="hisB"
                     /locus_tag="VM692_05230"
     CDS             complement(681..1739)
                     /gene="hisB"
                     /locus_tag="VM692_05230"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HUQ51601.1"
                     /translation="MRRILFVDRDGTLVAERKSLAEPVEQPLVAGVIPALLRLKSAGY
                     QFVIVTNQPGLGTPDNPRSAFERADEKLLALFASQGIRFIGNFICPHLPEAQCACRKP
                     GIGLVRDFVNASPLDRERSAMVGDRDTDVELARNLGIQGFKLGAPGGETWEAIAHALV
                     DRPRTAVVHRKTRETDIRVAVDLDSEAEPRAKTNLGFFDHMLEQLGKHGGFKLDVQCT
                     GDLHIDEHHTVEDVALTLGQALREALGDKRGIQRYGFLLPMDEADAEVSIDLGGRPYL
                     VFEAKFARDRVGDLPTELVPHFFRSLAETLGAAIHIRVRGDNTHHMVEVSFKAVARVL
                     RQALKRQGDELPTTKGQL"
     gene            complement(1732..>2068)
                     /locus_tag="VM692_05235"
     CDS             complement(1732..>2068)
                     /locus_tag="VM692_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUQ51602.1"
                     /translation="PTPSIELVEQALSKDSLRVSEERVGVLKSERERLAAALRELPQV
                     LHVYPSDANFILARTRDGQAFRETARRADILVRTFDDPLLKDCVRITVGRPSDNDQLL
                     RAVAGGTHA"
BASE COUNT          347 a          691 c          690 g          340 t
ORIGIN      
        1 gcaaagtcgc cgtagagcag ccgcacgcag cgcccgtcgc gtaggtcgat ggcgggtatc
       61 agatccatac gtttcagcta gttacagtcc tatcttcaga aaattatcga gtatctgggc
      121 gccaccgcgt gacgagcgct cggggtggaa ctgggtgcca taaaagttgc cctgctcgat
      181 gacggccgag aacggcacac cgtagctcgt gaccgcacgc gtgtacggcc ccggcggcgc
      241 cgcgtagctg tgtacgaagt acgcatacgt cccttgcgct acgccggcga gcagcggcga
      301 gtcgccgacc ggctcgatct cgttccaacc catctgcggc accgggagat cggcgcgcgc
      361 ggggaagcgg cgcacctcgg cgtcgatgac gccgagacag cgcgtctgtt cctcctcgga
      421 gctcgcgaac agcagctgca tcccgaggca gatgccgagc acgggctgcg aaagcgtcgg
      481 gatcagctcg acgagcccgg cggctgcgag ccgctgcatg ccgggagccg cagcgccgac
      541 tccgggcaga atcacgtgac tcgccgcgcg gatgcgcgcg gggtcctcgg tcagcgggac
      601 gtcggcgccg agccgctcga gcgcgaactg cagcgacgcg agattcgacc cgcacgacgg
      661 aatgatgacg gcgttcgcct tcacagctgg cccttcgtcg tcggcagctc gtcgccttgc
      721 cgcttcagcg cttgacgtag cacacgcgcg acggctttga acgacacttc gaccatgtgg
      781 tgcgtgttgt cgccgcgcac gcgaatgtgg atcgcggcgc cgagcgtctc ggcgagcgag
      841 cggaagaagt gcggcacgag ctccgtcggc aagtcgccga cgcggtcgcg cgcgaacttg
      901 gcctcgaaca cgagatacgg ccggccgccg agatcgatcg atacctcggc gtccgcttcg
      961 tccattggca gtaagaagcc gtagcgctga ataccgcgct tgtcgccgag cgcttcgcgc
     1021 agcgcttgcc ctaacgtcaa cgcaacgtcc tcgaccgtgt ggtgctcgtc gatgtgcaag
     1081 tcgcccgtgc actgcacgtc gagcttgaag ccgccgtgct taccgagttg ctcgagcatg
     1141 tggtcgaaga agccgagatt cgtcttcgcg cgcggctcgg cctcgctgtc gaggtcgacg
     1201 gcgacgcgaa tgtcggtctc gcgcgtcttg cggtgcacga ccgcggtgcg cggccggtcg
     1261 acgagcgcgt gcgcgatggc ctcccaggtt tccccgcccg gcgcgccgag cttgaagcct
     1321 tgaataccga gattgcgcgc caactcgacg tcggtgtcgc ggtcgccgac catcgccgag
     1381 cgctcgcgat cgagcgggct cgcattgacg aagtcgcgca cgagtccaat acccggcttg
     1441 cggcacgcgc actgcgcctc tggcaagtgc gggcagatga aattgccgat gaaccggatt
     1501 ccttgcgacg cgaaaagcgc caacagcttc tcatcggcgc gctcgaaggc cgagcgcggg
     1561 ttgtccggcg tgcccaaccc cggttgattc gtgacgatga cgaactgata cccggccgac
     1621 ttgaggcgca gcaaggcagg aatcacgccg gcgacgagcg gctgctcgac gggctccgcg
     1681 agactcttgc gttccgcgac gagcgtgccg tcgcgatcga cgaacaagat cctacgcatg
     1741 cgttccaccc gctaccgctc gcagcagttg gtcgttgtcg ctcgggcgcc ctaccgtgat
     1801 ccgcacgcaa tccttcagca gcgggtcgtc gaacgtccgc acgaggatgt cggcgcgcct
     1861 cgccgtctcg cggaacgcct gaccgtcgcg cgttcgcgcg aggatgaagt tggcgtcgct
     1921 ggggtagacg tgcagcactt gcggcaactc gcgcagcgcg gccgccagcc gctcgcgctc
     1981 gctcttcaac acgccgacgc gttcttcgga aacgcgcagc gagtccttcg acaaggcttg
     2041 ctcgacaagc tcgatcgacg gcgtcggg
//
LOCUS       DATLEE010000250         3223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9224603, whole genome shotgun sequence.
ACCESSION   DATLEE010000250 DATLEE010000000
VERSION     DATLEE010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9224603"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..719
                     /locus_tag="VM692_05240"
                     /pseudo
     CDS             24..719
                     /locus_tag="VM692_05240"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(772..2343)
                     /locus_tag="VM692_05245"
     CDS             complement(772..2343)
                     /locus_tag="VM692_05245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUQ51603.1"
                     /translation="MPLPPNVSRRDFGDALRAFAAAVGDEWVFSSDEDLALYRDAYSP
                     LREEPDERVASAAVAPATVEQVQAILRAANRYRVPLYPISTGRNLAYGGAAPVLSGSV
                     VLDLKRMNRVLEVDEANAYALVEPGVSYFDLYRHIQDRGMKLMLDVPTPGWGSAIGNA
                     LEHGVGLSPLRDHSAAQCGLEVVLADGSLLRTGMGAMPNAATWQQFKYGIGPHIDGLF
                     SQSNFGVVTKMGFWLLPEPEAVRGLRVIAARHDDVAPLIATLANLVYSGVIDSTFSLR
                     SPVLHGTRDADFDALVAAPDGGAAADWDRYAAARELSFWQLDLSFYGPEALVDLRWAH
                     TLERFRALAGVRFANAPTYRFPMSAAEREQVRDKQLLGIPSLEGFVADGADGHVDFSV
                     VVPMSGAAVLEALKVLGRVFAESQVDVGMGALTSFHARTLTFISSFPTRRDDREANRR
                     MREAYGRALDVATAHGWGQYRAHAAFMDRAVAAYSFNDGALARFHARLKDAVDPNGIL
                     AAGRYGIWPRDGGGG"
     gene            2448..2987
                     /locus_tag="VM692_05250"
     CDS             2448..2987
                     /locus_tag="VM692_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HUQ51604.1"
                     /translation="MNLTPVMTRFILHWGEMGTRWGVNRTVAQIHALLYLAPGPLPAD
                     EIAETLNVARSNVSTSLKELQSWGLVSVAHVMSDRRDHFAARSDPWEMLVTIMEQRKR
                     REIEPTLHVLRECVQELEKDRDTPKEARARIEGMLGFLTNVTTWFEQMLALPKSTVLT
                     LMKLGAKVANFVPKSSKDS"
BASE COUNT          561 a         1071 c         1083 g          508 t
ORIGIN      
        1 tcagcacgta cttgccactg acgatggcgg cgttgtttct ggccggggcg ggatgggtgt
       61 ctgcgcttgc aacgttcaac gttgcaattc agctgtccgc gccgcgttgg gtcgtggggc
      121 gggctttgtc cctttatcag atggccgcgt tcggcggcat tgcgggctga agctggatct
      181 ggggccgtgt cgcggagcag cagagcatcg agctcgcgct gctcgcgtgc gccgccgtgt
      241 tggtcgcctg cgcggcggtc ggcgcttggc gaccggtcgc gcaagcgaaa gatctcgacc
      301 tcgacccgct acgaccctgg caggagccgg aaacggccgt gtcggtcgac cctcggaccg
      361 ggcccgtcgt gatcacgatc gaatacgtca ttgccgagga cgaccaacta aaatttttgg
      421 cagcgatggc ggaacggcga cgcatccgcc ggcgcgacgg tgcgctgaac tggcgcttgc
      481 tgcgcgacct gtcggacgtg cagctctggg tcgagcgcta cgagacgccg acgtggctgg
      541 actacgcccg cctcaacagc cgaatgacgc gcaacgacgc ggtcgttccg gaatcgcttc
      601 gcgcactgca ccgcggcccc ggggctccgc gcgtccgacg catggtcgaa cgcccggtcg
      661 ccgctcagtc ggccggtctg gctgcgccag tacacgacgt ggccatcgtt ccggactgag
      721 cagcgagccc gggccggtgc tgctcgcgcc cgaagcgcac gaagcgcacg ctcatccgcc
      781 accgccgtct cgcggccaga tgccgtagcg gccggccgcg agaatgccgt tcggatcgac
      841 ggcgtctttg agccgggcgt ggaaccgcgc caacgcgcca tcgttgaacg agtacgcggc
      901 gacggcgcgg tccatgaacg ccgcgtgcgc ccggtactgt ccccagccgt gcgccgtcgc
      961 gacgtcgagc gcccgaccgt aagcctcgcg catgcgccga ttcgcttcgc ggtcgtcgcg
     1021 gcgcgtcggg aaagaagaga tgaacgtcag cgtgcgcgcg tgaaacgacg tcagcgcgcc
     1081 catgccgacg tcgacttggg actcggcgaa gacgcggccc agcaccttca gcgcctcgag
     1141 cacggccgcg cccgacatcg gcacgacgac ggagaagtcg acgtggccgt ccgcgccgtc
     1201 ggctacaaac ccttcgaggc tcggaatgcc gagcaattgc ttgtcgcgga cttgctcgcg
     1261 ctcggccgcg ctcatcggga agcgataggt cggcgcgttc gcgaagcgca cgccggcaag
     1321 cgcccggaag cgctcgagcg tgtgtgccca gcgtagatcg acgagcgcct ccgggccgta
     1381 gaaactcaag tcgagctgcc aaaacgacag ctctcgcgcc gcggcatagc ggtcccaatc
     1441 cgcggcggcg ccgccgtccg gcgcggctac cagcgcgtcg aagtcggcgt cgcgcgtgcc
     1501 gtgcagcacg gggctgcgca gcgagaacgt cgagtcgatg acaccggaat agacgagatt
     1561 cgcgagcgtc gcgatcaacg gcgcgacgtc gtcgtgccgc gccgcgatga cgcgcagccc
     1621 gcgcacggct tcgggctccg gcagcaacca gaagcccatc ttcgtgacga cgccgaagtt
     1681 cgactgcgag aacaagccgt cgatgtgcgg gccgatgccg tacttgaact gctgccaggt
     1741 cgcggcattt ggcatggcgc ccatgccggt gcgcaacaag ctgccgtcgg ccagcacgac
     1801 ctcgaggccg cactgcgccg cggaatggtc gcgtagcggc gacagcccga cgccgtgctc
     1861 gagcgcgttg cctatagctg agccccagcc cggcgtcggc acgtcgagca tgagcttcat
     1921 gccgcggtct tgaatgtgcc ggtacagatc gaagtagctg acgcctggct cgaccagcgc
     1981 atacgcgttg gcctcgtcga cctcgagcac acggttcatg cgcttcagat cgagcacgac
     2041 gctgcccgac aacacgggcg ccgcgccgcc gtacgcgagg ttgcggcccg tcgagatcgg
     2101 gtacagcggg acgcgataac gattggccgc ccgcaagatc gcctgcactt gctcgacggt
     2161 cgcgggcgcg acggctgccg aagccacgcg ctcgtcgggc tcctcgcgca gcggagagta
     2221 agcgtcgcgg tagagcgcga ggtcctcgtc ggacgagaac acccactcgt cgccgacggc
     2281 cgcggcgaac gcgcgaagcg cgtcgccaaa gtcgcggcgg ctgacgttgg gcggcagggg
     2341 cacggcgcaa ctatacccgc cggtcggttc ccgacgaccg cgccgcgggc ttgactcttt
     2401 acccgtctag gtttatcgtt atttcagaca acacagaaat aacagaaatg aacctgactc
     2461 ccgtcatgac gcgtttcatc ctgcactggg gcgagatggg cacccgttgg ggcgtcaatc
     2521 gcaccgtcgc gcagattcac gcgctgctgt atctggcgcc gggcccgctg cccgccgacg
     2581 agatcgccga gacgttgaac gtcgcgcgct cgaacgtcag cacgagcttg aaagagctgc
     2641 aaagttgggg gctcgtcagc gtcgcgcacg tcatgagcga ccgccgcgat catttcgcgg
     2701 cgcgctccga tccctgggag atgctcgtga cgatcatgga gcagcgcaaa cggcgcgaga
     2761 tcgagccgac gctgcacgtg ctgcgcgagt gcgtgcagga gctcgaaaag gaccgcgaca
     2821 cgccgaaaga ggcgagggcg cgcatcgagg gcatgctcgg tttcctgacg aacgtcacga
     2881 cctggttcga gcagatgctc gcgctgccga agtcgaccgt gctgacgctg atgaagctcg
     2941 gcgccaaggt cgcgaacttc gtgccgaaaa gctcgaagga ttcctaacgg ggcctttttt
     3001 tgcccagtga gttctgcgac gacagaaaag actgtaaaca acgcaaggag aaacgtcgtg
     3061 atcatcgaaa ccacttacgt ctgctatctc gcgatcagcg tcgccttgac cgtctgggtc
     3121 gcgcgcacgc tgagcaagaa cggccgcatc tttctagtca acagcttcgc gagcgacgag
     3181 cggctcgccg actccgtcaa ccacctgctc gtcgtcggct tct
//
LOCUS       DATLEE010000251         3999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_287243, whole genome shotgun sequence.
ACCESSION   DATLEE010000251 DATLEE010000000
VERSION     DATLEE010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287243"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..401)
                     /locus_tag="VM692_05255"
     CDS             complement(177..401)
                     /locus_tag="VM692_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51605.1"
                     /translation="MDKWEFFQDAAGLWRWRCTSADGHRVFNSKRSHPSRAHAVAEAT
                     TRGYGDEGVQADLDETSVTLQALRLDNDAP"
     gene            474..881
                     /locus_tag="VM692_05260"
     CDS             474..881
                     /locus_tag="VM692_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUQ51606.1"
                     /translation="MQQSYTIGQLATEAGVNVETIRFYQRRKLFPQPPRTHGRMRRYG
                     GTEVKRLRFIKCAQRLGFTLKEINDLLELLAPVSCSATRKVAAAKLELVDERIRALQE
                     LRREFTQLLAACDSNTDESRCPIIEHFAGGANA"
     gene            886..1626
                     /locus_tag="VM692_05265"
     CDS             886..1626
                     /locus_tag="VM692_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUQ51607.1"
                     /translation="MRRRAVLLGIALLPIAAAAEEAPVKIVATIAVQGALTEIESLLP
                     ARSGVPVEVEFATTAALVERLARGEAADLAVLTKDAVLELAAQGRVRESTDLVLSVIG
                     LAVADDAPLPAMKTAADFVAFMKATPSIAYTVRGVSGLHMAQLIERFELDDVVKPKAT
                     VVDGFAGTPLREGKVAAAVQQISELKFAGATKIVPLPDELQVRTIFTLAALNGTPRAE
                     AASKVVRALTSPEAAAAYERAGSSPLFK"
     gene            1654..2541
                     /locus_tag="VM692_05270"
     CDS             1654..2541
                     /locus_tag="VM692_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HUQ51608.1"
                     /translation="MRINSVLLALIVSAPASIGHAQTLRGAIDMHAHAAPDGTPRKVD
                     ALELATLAKAAGMRAVVIKNHYEPTASLAYVVRKAVPGIEVFGGISLDLTVGGVNPAA
                     VEWMTKVEGGYGKIVWLPTFDSESQVKLTGQQRPFAPVTRDGKILPEVLAVIALAAQH
                     DLVLETGHSAPQESLLIIAEAKRQNVKHVLVTHAMNSPGGSMTLDQMREAARLGSLLE
                     LVYSPLTDEQLVKEADAIKTIGAASFVLSSDLGQPPNPLHTDGLLALYKGLMAHGISA
                     ADIAIMSSANPARLLGLQP"
     gene            2607..3062
                     /locus_tag="VM692_05275"
     CDS             2607..3062
                     /locus_tag="VM692_05275"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUQ51609.1"
                     /translation="MANYAKRIEASAMFAAALVGAAALPIVAQDSAPARAAPPVLLSA
                     PLADAPGKNLVVVELNFPPNARPASTPENHPRGHRHPGSVYVYVTQGSVRLGVEGQPV
                     QVVAAGGSFFEAVGAHHIIAENASATEPARAIAVMVVPDGAPLTTLDPQ"
     gene            complement(3064..3726)
                     /locus_tag="VM692_05280"
     CDS             complement(3064..3726)
                     /locus_tag="VM692_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51610.1"
                     /translation="MRTAVVSLIAAASFTGAALAQAPAARQLPPDVNPVSLSRLPPLT
                     RDDLDAEGKEIYDYIVKDGPAPKTGPVALSLYSPKIAKVFSDLNGYLRYNGDLSARHT
                     EVAICVATWEIQQQYEYSAHEPAALRYGAPQAVIDTIKYNREPVGLPPEETMIIKLGR
                     AIMRDHKVDSALYADAVKMFGRKGLVELVTVMGDYVMVGMVMTAIDQQLPADRPAALP
                     PR"
     gene            complement(3861..>3999)
                     /locus_tag="VM692_05285"
     CDS             complement(3861..>3999)
                     /locus_tag="VM692_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HUQ51611.1"
                     /translation="ERARTALGDRFDIRRFHDTVLGGGALPLSILERRVDAWIATERA
                     R"
BASE COUNT          665 a         1300 c         1371 g          663 t
ORIGIN      
        1 tcggcaaagg ctttggccaa gcgactcagg atctcctcgg gcagcaaagc cgcggagccc
       61 aagccggcgt gcgagagcgt cgcaacgcag cggtcgtagt cggagtgcgc cgcgaggtac
      121 ttcgccttca gggccgtcag ctcgtgttcc gtgaccttcg ccatgcgcga cggcgcctac
      181 ggcgcgtcgt tgtcgagacg cagcgcctgc agggtgacgg acgtctcgtc gaggtcggct
      241 tgcacaccct cgtctccgta gccgcgcgtc gtggcctcgg cgacggcgtg cgcgcgcgac
      301 gggtgcgagc gcttcgagtt gaatacgcgg tggccgtcgg cggacgtgca gcgccaccgc
      361 caaagccctg cggcgtcctg aaagaactcc cacttatcca tctgactggt tagaaatttt
      421 ttcgggtgag cttccggtac gataccggcc gtaccatggt acggagtcaa gcggtgcagc
      481 agagctacac gatcgggcaa ctcgcgacgg aagccggcgt gaacgtcgaa accattcgct
      541 tctaccaacg gcgcaagttg tttccgcaac cgccacgcac gcacggccgc atgcggcgtt
      601 atggcggtac cgaagtcaag cgcttgcgct tcatcaagtg cgcgcagcgt ctcgggttca
      661 cgctaaaaga gatcaacgat ctgttggagt tgctcgcgcc cgtgtcatgc agcgcgacac
      721 gcaaggttgc agcggccaag ctcgagctcg tcgacgagcg catccgcgcg ctgcaagagc
      781 tgcgtcgaga gttcactcaa ctgctcgccg cttgcgattc gaacaccgac gagtcgcgct
      841 gcccgatcat cgagcatttc gcgggcggcg cgaatgctta gcgtcatgag gcgacgtgcg
      901 gtgctgctgg ggatcgcgct gctgccgatc gcggcagctg ccgaagaagc gcccgtcaag
      961 atcgtcgcga cgattgccgt gcaaggcgcc ctgaccgaga tcgagtcgct gttgcctgcg
     1021 cgctcgggtg tgcccgtcga ggtcgagttc gcgacgacgg cggcgctcgt cgagcgactc
     1081 gcgagaggtg aagccgcgga tctcgccgtg ctcacgaaag acgcggtgct cgagctcgct
     1141 gcgcaagggc gcgtgcgcga atcgacggac ctcgtgctgt cggtcatcgg cctagccgtg
     1201 gccgacgacg cgccgctgcc ggccatgaag acggctgcgg atttcgtcgc cttcatgaaa
     1261 gcgacgcctt cgatcgctta cacggtgcgc ggcgtcagcg gtttgcacat ggcgcagctg
     1321 atcgagcggt ttgagctcga cgacgtcgtg aagccgaaag cgaccgtcgt cgacggcttc
     1381 gcgggcacgc cgttgcgcga aggcaaggtt gcggcggccg tgcagcagat cagcgagctg
     1441 aagtttgccg gcgcgacgaa gatcgtgcca ctgcccgacg agcttcaagt gcgcacgatc
     1501 ttcacgctgg ccgcgttgaa cggcacaccg cgcgcggaag cggcgagcaa agtcgttcga
     1561 gcgttgacgt cgcccgaggc cgctgccgcg tacgagcgcg ccggctcgtc gccgctcttc
     1621 aaataaccgg actcgtcgaa cgggagtgcc gtgatgcgta taaactctgt gctgctcgcc
     1681 ctgatcgtct cagctccggc gtcgatcggc catgcgcaga ctctgcgcgg cgccatcgac
     1741 atgcacgcgc atgcagcacc ggacggcacg ccgcgcaagg tcgacgcgct cgagctcgcg
     1801 acgctcgcca aagcggccgg catgcgtgcc gtcgtcatca agaaccacta cgagccgacg
     1861 gcctcgctgg cgtacgtcgt gcgcaaagcc gtgcccggca tcgaggtgtt cggcggcatt
     1921 tcgctcgact tgaccgtcgg cggcgtgaat cccgcggccg tcgagtggat gacgaaggtc
     1981 gaaggcgggt acggcaagat cgtgtggctg ccgacgttcg actccgagtc gcaggtgaag
     2041 ctcacgggcc agcaacggcc gttcgcgccc gtgacgcgcg acggcaagat cctgcccgag
     2101 gtgctcgccg tcattgcgct cgcggcgcag cacgacctcg tgctcgagac ggggcattcg
     2161 gcgccgcagg aatcgctgct gatcatcgcc gaggcgaagc gccagaacgt gaagcacgtg
     2221 ctcgtgacgc atgcgatgaa cagccccggc ggctccatga cgctcgacca gatgcgcgaa
     2281 gcggcacgac tcggctctct gctcgagctc gtctattcgc cgctgaccga cgagcagctg
     2341 gtcaaggagg ccgacgccat caagaccatc ggcgcggcgt cgttcgtgct gtcgagcgac
     2401 ctcggccagc cgccgaaccc gctgcatacc gacggcctgc tcgctctata taaagggctg
     2461 atggcgcacg gcatctccgc cgccgatatc gccatcatgt cgagcgctaa ccccgcaagg
     2521 ctgttgggac tgcaaccgta gcttgcatgc agaacctgat caccaggcat ctagagcgcc
     2581 ggagcgtgcg gcgataggag atcgatatgg cgaactacgc gaagcgaatc gaggcgtcgg
     2641 ccatgttcgc ggcagcattg gtcggcgcgg ccgcgctgcc catcgttgcg caggacagtg
     2701 cgccggcgcg cgccgcaccg ccggttttgc tatcggcgcc gctggccgac gcgcccggca
     2761 agaacctcgt cgtcgtcgag ctcaactttc cgccgaacgc gcggccggcg tcgacgcccg
     2821 agaaccaccc gcgcgggcac cggcatccgg gctcggtgta cgtgtacgtg acgcagggtt
     2881 ccgtgcgcct gggagtcgag ggccagcccg tgcaagtcgt cgcggcaggc ggcagcttct
     2941 tcgaagccgt cggtgcgcat cacatcatcg cggagaacgc gagcgcgacg gagcccgcgc
     3001 gggcgatcgc cgtgatggtc gtccccgacg gagcgccgct gaccacgctc gatccgcagt
     3061 gagtcagcgc ggcggcagcg ccgcgggccg gtcggccggc aactgctgat cgatcgccgt
     3121 catgaccatg ccgaccatga cgtagtcgcc catgaccgtg acgagctcga ccaagccctt
     3181 gcgcccgaac atcttgacgg cgtccgcgta gagggccgag tcgaccttgt ggtcgcgcat
     3241 gatggcgcgg ccgagcttga tgatcatcgt ctcctcgggc ggcaggccga cgggctcgcg
     3301 gttgtacttg atcgtgtcga tgaccgcctg cggcgcgccg tagcgcagcg ccgcgggctc
     3361 gtgcgccgag tactcgtact gctgctggat ctcccacgtc gcgacgcaga tcgcgacttc
     3421 ggtatgccgc gcgctcaagt cgccgttgta gcgcaggtag ccgttcaggt cgctgaacac
     3481 tttcgcgatc ttcgggctgt acagcgacaa cgctacggga cctgtcttcg gggccggacc
     3541 gtccttgacg atgtagtcgt agatctcttt gccctcggcg tcgaggtcgt cgcgcgtcaa
     3601 tggcggcaag cgcgacagcg atacggggtt cacgtccggc ggcagttgcc gtgcggcggg
     3661 cgcttgggcc aaggcggcgc ccgtgaaaga ggcggcggcg atcagcgaga cgacggcggt
     3721 gcgcatacgg tgttcctgtt gttgctcgag tgaagttagc gcgagtgtag atagcggccc
     3781 tcgttcaagg caaacgagtc aagggcgttg ggggcggcgc gacggcgcac tcaggatgcc
     3841 gagcggaacg tacgttgcgc tcagcgcgcg cgctcggttg cgatccatgc gtcgacgcgc
     3901 cgctcgagga tcgacagcgg caatgcaccg ccgccgagca ccgtgtcgtg gaagcggcgg
     3961 atgtcgaacc ggtccccgag cgccgttcgc gcgcgctcg
//
LOCUS       DATLEE010000252         3188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9227302, whole genome shotgun sequence.
ACCESSION   DATLEE010000252 DATLEE010000000
VERSION     DATLEE010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9227302"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1525)
                     /locus_tag="VM692_05290"
     CDS             complement(137..1525)
                     /locus_tag="VM692_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HUQ51612.1"
                     /translation="MAEAAAANDLDALDLARVLHPNTNLAALHRGKPLVLVRGRGVHV
                     FDNHGKQYIEAMAGLWCTTLGYGDDELARTAYEQLKKLSFSHMFTGKSHEPGILLADK
                     LVQMAPFAASRVFFGNSGSDANDTQIKLVWYYNNALGRPKKKKIISRLKGYHGTTLGS
                     AGLTGLPSFHKLFDVPLPPFLHTDAPYFYRGAQPGESEEDYSTRLAANLEHLIVREGP
                     DTVAAFIAEPLMGVGGVLLPPRGYFAKVQAVLAKHDVLLIDDEVITGFGRTGEIWGAQ
                     AFGMRPHTLTAAKALSSAYLPISAVVVPEFLYAPMIEASGEVGLFGHGFTYSGHPVAA
                     AVALRALGIYEERKLYEHVRRIAPQFQTALAELAAHPLVGDTRGIGLLGACELVQNKA
                     TKAAFEAKLAVGAKAMHFCQEHGLIVRAIGDVIALCPPYIVTSTDIDEIFAKLRNGLD
                     DTLAWVQRERLL"
     gene            complement(1540..>3188)
                     /locus_tag="VM692_05295"
     CDS             complement(1540..>3188)
                     /locus_tag="VM692_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HUQ51613.1"
                     /translation="RAIRASMSIPAALSPIEIDGRLLVDGGIAMNLPVDVAREMGADV
                     VIAVDISSQLLGRDTLKSVLDVTAQLTNLLTRGGTLEQQRKLTANDVLLLPQFDEELS
                     STNFTRMREAIQSGYDAVMQRRAEFERFALSEEQYAAHITERTDPRMRQLPVVDFVRL
                     DNHAPVADSVVEARLGAIKLGEPLDVDQVERAMNTVYGLDYYQNVRYGLVNDTGRTGI
                     EVELDERSWGPNYLQLGMEYSSAADSDALFGLAASYLSTAINPLGGEWRATLVIGDEP
                     AFVTDLYQPIGPKGLYFYAPSLNLTSTQFNVYVGDDRVTEAQLRETTLEFAVGRELPT
                     WGEYRFGVRAARGAIDLRVGDPSFISEETFHRGEFFGRFSVDTMDSVAFPRQGSSVTM
                     EWRASRDRPFAADADFDQLLLASAYAKTWGRHTVLTTLRYDATISGTAPNSRLFRMGG
                     FFDISGINRNQLSGQYATRLGAAYYRRIGDFALFPAFAGMSIELGNVWNSRKDISASD
                     SIFGGSFWAGVDTPIGPVYLGYGRAEGGEDAFYVSLGRVF"
BASE COUNT          569 a         1069 c         1025 g          525 t
ORIGIN      
        1 ctcgaacggc cataacgggc tcagcgccac tcggaatgcc tgccaaccca ggtagtcgtc
       61 cgcgacgggc aggaaccagg agaccaagtt agtcacgacg gcagcaagcc aagcgaaatg
      121 cggcgccttc ataccgttac agcaaccgct cgcgctggac ccaggccagg gtgtcgtcga
      181 ggccgttgcg cagcttcgca aagatttcgt cgatgtccgt cgacgtcacg atgtagggcg
      241 gacacagcgc gatgacgtcg ccgatcgccc gcacgatgag gccgtgctct tggcaaaagt
      301 gcatcgcctt cgcgccgacg gcgagcttcg cctcgaatgc cgctttcgta gccttgttct
      361 gcacgagctc gcaggctccg agcaagccga tgccgcgcgt gtcgccaacc aacggatgcg
      421 ccgcgagctc ggcgagcgcc gtttggaact gcggcgcgat ccgccgcacg tgctcgtaga
      481 gcttgcgctc ctcgtagatt ccgagcgcgc gcaacgcgac ggccgccgcg acgggatgcc
      541 ccgagtacgt aaagccgtgg ccgaacaagc cgacctcgcc gctcgcctcg atcatcggcg
      601 cgtagaggaa ctccgggacg acgacggcgc tgatcggcag gtacgccgac gacagcgctt
      661 tggccgccgt cagggtgtgc ggccgcatgc cgaacgcttg cgcgccccaa atctcgccgg
      721 tccggccgaa gcccgtgatg acctcgtcgt cgatcagcaa gacatcgtgc ttcgcgagca
      781 cggcttggac tttcgcaaag tagccgcgcg gcggcagcag cacgccgccg acgcccatca
      841 gcggctcggc gatgaacgcc gcgacggtat cgggcccctc gcgcacaatg aggtgctcga
      901 ggttggccgc gagccgcgtt gaataatcct cttcgctttc gccgggctgc gcgccgcggt
      961 agaaataagg cgcgtcggtg tgcaagaacg gcggcaacgg cacgtcgaag agcttgtgaa
     1021 acgacggcaa gcccgtgagc cccgccgagc ccagcgtcgt gccgtgatag cccttcagcc
     1081 gcgagatgat cttctttttc ttcggccgcc cgagcgcatt gttgtaatac cagacgagct
     1141 tgatctgcgt gtcgttggca tcggagcccg aattgccgaa gaacacgcgc gacgcagcga
     1201 acggcgccat ctgcacgagc ttgtcggcca gcaagatgcc gggctcgtgg ctcttgcccg
     1261 tgaacatatg cgagaacgac agcttcttca gctgctcgta cgccgtgcgc gcgagctcgt
     1321 cgtcgccata gccgagcgtc gtgcaccaca gcccggccat cgcctcgatg tactgcttgc
     1381 cgtggttgtc gaagacgtgc acgcccctgc cgcgcacgag cacgagcggc ttaccccggt
     1441 gcagcgccgc gagattggta ttcgggtgca ggacgcgcgc caaatccaac gcgtcgagat
     1501 cgttcgccgc tgccgcttcg gccatcgcag tctccttgct tagaacacgc ggccgagcga
     1561 cacgtagaac gcgtcctcgc caccctcggc gcgtccgtaa ccgagataca cggggccgat
     1621 cggcgtgtcg acgccggccc aaaacgagcc gccgaaaata ctgtcgctcg ccgagatgtc
     1681 cttgcgagag ttccacacgt tgccgagctc gatgctcatg cccgcgaatg cagggaacaa
     1741 cgcgaaatcg ccgatgcgcc gataataggc ggcacccaag cgcgtcgcat attgcccgga
     1801 aagctgattg cggttgatgc cggagatgtc gaagaacccg cccatgcgga acaaccggct
     1861 gtttggcgcc gtgccgctga tcgtcgcgtc gtagcgcagg gtcgtcagca cggtgtgacg
     1921 accccacgtc ttcgcgtacg cggacgccag cagcaattga tcgaagtcgg cgtcggccgc
     1981 gaacggccgg tcgcgcgacg ctcgccactc catcgtgacc gacgacccct gccgcggaaa
     2041 ggccacgctg tccatcgtgt cgacggagaa gcggccgaag aactcgccgc ggtgaaacgt
     2101 ttcctccgag atgaagctcg ggtccccgac gcgcagatcg atggcaccgc gagccgcacg
     2161 aacaccgaat cgatattcgc cccaggtcgg cagctcgcgg cctaccgcga actcgagcgt
     2221 cgtttcccgc agctgcgcct cggtgacgcg gtcgtcgccg acgtagacgt tgaactgtgt
     2281 cgaggtgaga ttcagcgacg gcgcatagaa gtacaggccc ttcggaccga tcggctggta
     2341 cagatccgtc acgaacgccg gctcatcgcc gatcaccagc gtcgcccgcc actcgccgcc
     2401 gagcgggttg atcgccgtgc tcaagtagct cgcggcgagc ccgaacaaag cgtccgagtc
     2461 ggccgcggag ctgtactcca tgccgagctg caagtagttg gggccccacg agcgctcatc
     2521 gagctcgacc tcgatacccg tgcggcccgt gtcgttgacg agcccgtagc gcacgttctg
     2581 gtagtaatcg aggccgtaga cggtgttcat cgcccgctcg acctgatcga cgtcgagcgg
     2641 ctcgccgagc ttgatggcgc cgagcctcgc ttcgacgacg ctgtcggcca cgggcgcgtg
     2701 gttgtcgagg cgcacgaagt cgacgacggg cagctgccgc atacgcgggt cggtgcgctc
     2761 cgtgatgtgg gccgcgtact gctcttcgct gagcgcgaag cgttcgaact ccgcgcgccg
     2821 ctgcattacg gcgtcgtagc cgctctggat cgcttcacgc atgcgcgtga aattcgtcga
     2881 gctcaattcc tcgtcgaact gcggcagcag caagacgtcg ttcgccgtga gcttgcgctg
     2941 ctgctcgagc gtgccgccgc gcgtcagcag gttcgtgagc tgcgcggtga cgtcgagcac
     3001 cgatttgagc gtatcgcggc ccagtaactg cgacgagatg tcgacggcga tgacgacgtc
     3061 ggcgcccatc tcgcgcgcga cgtcgaccgg caaattcatc gcgatgccgc cgtcgacgag
     3121 cagccggccg tcgatctcga tcggcgacag cgccgcaggg atcgacatgc tcgcgcgaat
     3181 cgcgcgcg
//
LOCUS       DATLEE010000253         2031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_289846, whole genome shotgun sequence.
ACCESSION   DATLEE010000253 DATLEE010000000
VERSION     DATLEE010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289846"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="VM692_05300"
     CDS             complement(<1..1378)
                     /locus_tag="VM692_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ51614.1"
                     /translation="MVLSTPWAAHATGVGVAAAAREADVGAVRAQVAAGTDVNAPDES
                     GTSALLWAAYNASPELVSTLLAAGADPNTANPFGVTPLLQAARNGDAATTRLLLEAGA
                     GVNAVRDGETPLMAAARAGSVDAVQQLLEHGADPNASETLQSQTALMWAVAEGHLPVV
                     DALLQGGADPNAQARVSELKKRSMRADFPTGGFTALMWAARNGNEPIVRRLVDAGADV
                     KAVDGDGASAMMLAIVNDRFDMALTLLELGADANDGSLYYAIVMRDAPTDWRARDGSR
                     MRAAHDNRATALDLVARLLDAGADPNKPFSGQMHSASMCCDPQGSGTPFFRAAIAADV
                     ESLKLLVAHGADLEWTPKPVEGAPPNPLGDNTGLTPLMVALNGGKGQLMDGGPGDIRE
                     GKIGIFREPGDREPADAVRVLLEAGANPNAVTLKGDSALHIAAHDGRLGPIRELVAHG
                     ASVKLRNKD"
BASE COUNT          312 a          717 c          674 g          328 t
ORIGIN      
        1 cgtccttgtt gcgcagcttg accgacgccc cgtgcgcgac gagctcgcgg atcggtccga
       61 gccggccgtc gtgggcggcg atgtgcagcg cggagtcgcc cttcaaggtc acggcgttcg
      121 gattcgcacc ggcttcgagc aacacgcgca cggcgtccgc cggctcgcga tcgccgggct
      181 cgcggaaaat accgatcttg ccctcgcgga tgtcgccggg gccgccgtcc atgagctgac
      241 ctttgccgcc gttcaaggcg accatcagcg gcgtgagccc cgtgttgtcg ccgagcggat
      301 ttggcggcgc gccttcgacg ggcttcggcg tccattcgag atccgcgccg tgcgcaacga
      361 gcagcttcag cgattcgacg tcggccgcga tggccgcgcg gaagaacggt gtgccggacc
      421 cttgcggatc gcagcacatc gaggccgagt gcatctggcc ggagaacggc ttgttcggat
      481 ccgcgccggc gtcgagcagc cgcgcgacga ggtcgagcgc cgtggcgcga ttgtcgtgag
      541 ccgcgcgcat tcgcgagccg tcgcgtgcgc gccaatccgt cggtgcgtcg cgcatcacga
      601 tcgcatagta gagcgagccg tcgttcgcgt cagcgccgag ctcgagcagc gtcagcgcca
      661 tgtcgaagcg atcgttgacg atcgcgagca tcatcgcgct ggcgccgtcg ccgtcgacgg
      721 ccttcacgtc ggcgcctgcg tcgacgaggc gtcgcacgat cggctcgttg ccgttgcgcg
      781 ccgcccacat caacgccgtg aaaccgcccg tcggaaagtc ggcgcgcatg ctgcgcttct
      841 tgagctccga gactcgcgct tgcgcgttcg ggtctgcgcc gccctgcagc agcgcgtcga
      901 ccacgggcag atgcccttcc gccacggccc acatcaacgc tgtttgactc tggagcgtct
      961 cgctcgcgtt ggggtcggcg ccatgctcga gcaactgctg gaccgcgtcg acgctgcccg
     1021 cgcgcgcggc cgccatcaac ggggtctcgc cgtcgcgaac ggcgttcacg ccggctcctg
     1081 cttccagcag cagccgagtc gtagccgcgt cgccgttgcg cgcggcttgc agcaacggcg
     1141 tgacaccgaa tggattcgcc gtgttcggat cggcaccggc ggctagcaac gtggagacga
     1201 gctcgggcga cgcattgtag gcggcccaca agagtgcgga cgtgccgctc tcgtccggcg
     1261 cattgacgtc ggtgccggcc gctacctgtg cccgcacggc gccgacatcg gcttcgcgcg
     1321 ccgcagccgc aacgcccacg cccgtcgcgt gtgccgccca aggcgtcgaa agaaccatta
     1381 gcgagactgc gagtgccccg tgggaacgct tgcgtccctg cgatcggttt tgcgcgtcaa
     1441 ccatgtcgtt ctccgcgccg gcacgggagc aaaagcatgc cggatatgag gtaaccgaat
     1501 atagcagatg gctagagaca cagtacccgc cgcagaaccc gtgtgcaaaa attacagcga
     1561 cgatgacgcc cgcgatcgtt aacgccgaga tcaactacac cgccgcaatg cgcgaccggc
     1621 cgtgtttcca cgccaacgat cagtcgctcg atcgtgtggc gctcgacccg cgcgtcgtcg
     1681 cgatccgcga cgcgcgttcc gaccgcaacc gcccgacact cgagctcgaa gggctcgagc
     1741 tcttacgctg cccgacccgc gtcgccgacg ccgtcgccgt gacggggccc ggggtgctgc
     1801 gtttcggcga gcggtcgcaa ggcttatgcg cgcttcgacc gcgacggcaa aatccattgg
     1861 acgttcgagg cgctgctgtt gcgctacaac cccgagcatc gcgggctgtt cttctcggac
     1921 atgcagcgcg acgacgcgct cgtgttcaag cgccacgaca ccgatcccga cgagccgcat
     1981 cacgtgccgc atagcgcgtt cacggacccg cgagtgccgg ccggcacgcc g
//
LOCUS       DATLEE010000254         3597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6853628, whole genome shotgun sequence.
ACCESSION   DATLEE010000254 DATLEE010000000
VERSION     DATLEE010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6853628"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1781
                     /locus_tag="VM692_05305"
     CDS             <1..1781
                     /locus_tag="VM692_05305"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ51615.1"
                     /translation="RGGTRVKHTFPADGEYVFQGRLVRGVEEGLFGVEGHDRPHEFLV
                     LVDGKTVFSAEVGGAELHELSVSEGINVAQDAVNAAMTSPPVPVTAGPHEVAFTWRER
                     SAREQNSWEPGLRASLEAHNPSGMPRLEDGVVEGPYNVTGVSDTPTRERLYVCQPASA
                     AEESACAQQILSSLARRAFRRPVDSEDIEASLAFYNDARADGGDFDSGIRAAVSRMLV
                     SPWFLFRVESDSPDAVAGSDHAISDFELASRLSFFLWSSIPDDELLGLAEKGQLREPS
                     VLEAQVRRMIGDQRAEAMVENFVGQWLQLRNLESRVKPDFLLFPDFDDNLRKAFRRET
                     ELLFANVLREGHSVHELINANYTFVNERLARHYGIKGVYGARFRKVDVADPNRYGLFG
                     HGSLLALTSAASRTSPIIRGKFIVTEFWNNPPPTPPADVPALEESAPKNRPSTVREQL
                     ELHRANPTCAACHNNIDPVGFALENFDVDGSWRETTREGLDIDSAGILADGTPVDGPV
                     QLRAALLADPELFANTVTEKMLIYALGRGLEPADMPVVRGIVRNAAEHDYSLVSIVLG
                     IVDSYPFQMRTNGGTAAAETVAQTRE"
     gene            1785..3134
                     /locus_tag="VM692_05310"
     CDS             1785..3134
                     /locus_tag="VM692_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ51616.1"
                     /translation="MFITKKHLDRRTFLRGALGTAIALPLLDAMVPAGTAQAQTAAAQ
                     RFRFGAIYMPNGVYPGLWHPTKVGKNFDFNRTMTPLEPYREYLTTISKLKAPEGRKDM
                     GGIHMGASAAFLNGTGPLTHNGDFNLIESKKSIDQYIADKIAGDTPLRSLELGTEDMG
                     TSAGACDGYPCVFFNTMAWYDDSSPLPVSVNPQATFERMFGDPGTPEQRLARLHAKQS
                     MLDSVMQETSRLQKVIGAKDNAILDEYLTNIRRVEEQLQKMQARSGALTNAAGGPVGI
                     PEDFNEHMTVTYDLLHLAFQGDITRVFSFMLGHEGSGRSYAHVGVKEPHHSTSHHKNT
                     PESLEQYARIGAYHMVKLGEFAEKLKNTPDGDGSLLDSSLIYFGAGMSNGLLHDRHNV
                     PAALLGRANGRLAGNRHIEATVDQPTSDLLLGMADVVGAEVETIGIATGRLNLASAS"
     gene            3208..>3597
                     /locus_tag="VM692_05315"
     CDS             3208..>3597
                     /locus_tag="VM692_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51617.1"
                     /translation="MKPGILNSVLVEQVRNSAAWLWLDPRCAALTAVFDAAYYEPLEE
                     PASLLRAFRLRLAAHYATVATFVPTDVDAHIRHHTWVAITDADVFSQARLCVDEAAAR
                     DAHLVSERVTQGLSGHDGEWFSVRAGAL"
BASE COUNT          638 a         1159 c         1167 g          633 t
ORIGIN      
        1 ctcgcggcgg cacgcgcgtc aagcacacgt tccccgccga tggcgaatac gtcttccagg
       61 ggcggctcgt gcgcggcgtc gaggaaggct tgttcggtgt cgaaggtcac gaccgtccgc
      121 acgagttcct cgtgctcgtc gacggcaaga ccgttttcag cgccgaggtc ggcggagcgg
      181 agctccacga gctcagcgtg tcagagggca tcaacgttgc gcaggacgcg gtcaacgcgg
      241 cgatgacgtc gccgcccgtg ccggtaacgg cagggccaca cgaggtcgcc ttcacgtggc
      301 gcgaacgttc ggcgcgcgag caaaattcgt gggagccggg cctgcgcgct tcgctggagg
      361 cgcacaaccc gtccggcatg ccgcggctcg aagatggcgt cgtggagggt ccgtacaacg
      421 tgacgggcgt cagcgatacc ccgacgcgcg agcgtttgta cgtctgtcag cccgcgagcg
      481 cggccgagga atcggcttgc gcgcagcaaa tcttgtcctc gctggcgcgc cgtgcgttcc
      541 gccggcccgt cgactcggaa gacatcgaag cgtcgctggc gttctacaac gacgcgcgtg
      601 ccgatggcgg cgacttcgac agcggcatcc gtgcggcggt ctcacgcatg ctcgtgagcc
      661 cgtggttcct gttccgcgtc gaaagcgatt cgcccgatgc ggttgccggc tcggatcatg
      721 cgatcagcga tttcgagctc gcgtcgcgct tgtcgttctt cctctggtcg agcattcccg
      781 acgacgagct gctcggcttg gccgagaagg gccagctacg cgaaccgagc gtgctcgagg
      841 cgcaagtgcg ccgcatgatc ggggatcagc gtgccgaggc gatggtcgag aacttcgtcg
      901 gtcagtggct gcagctccgc aacctcgagt cgcgcgtcaa acccgacttc ctcttgttcc
      961 cggatttcga cgacaacctc cgcaaggcct tccgtcggga gacggagttg ctgttcgcca
     1021 acgtgctgcg cgagggtcat tccgtgcacg agctcatcaa cgcgaactac acgttcgtga
     1081 acgagcggct ggcgcggcac tacggcatca agggcgtcta cggcgctcgc ttccgcaagg
     1141 tcgacgtcgc ggacccgaat cgctacggct tgttcggcca tgggagcttg ctggcgctga
     1201 cgtccgccgc gagccgcaca tcgccgatca tccgcggcaa gttcatcgtc acggaatttt
     1261 ggaacaatcc gccgccgacg ccgccggccg acgttccggc gctcgaagag agcgcgccga
     1321 agaatcggcc gtcgacggtt cgcgagcaat tggagctgca ccgtgcgaac ccgacctgcg
     1381 cggcgtgtca caacaacatc gaccccgtcg gctttgcgct cgagaacttc gacgtggatg
     1441 gtagctggcg cgagacgacg cgcgagggcc tcgacatcga ttccgcaggc atcctggccg
     1501 acggcacgcc cgtggacggc ccggttcagc tgcgcgctgc cctgctggcg gaccccgagc
     1561 tgtttgcgaa cacggtcacg gaaaagatgc tcatttacgc actcggtcgc ggtttggagc
     1621 cggcagacat gccggtcgtc cgcggaatcg tgcgaaatgc ggccgagcac gattacagcc
     1681 ttgtctctat tgtattaggt atagtagata gctatccgtt ccagatgcga acgaatggcg
     1741 gaaccgctgc ggcagaaact gtcgcgcaaa cgagggagta gccaatgttc attacgaaga
     1801 agcacttaga tcgccgtacc tttctgcgcg gtgctctcgg cacggccatt gcgctgccgt
     1861 tgctcgacgc gatggtgccg gccggaacgg cccaggccca aacggcagcc gcgcagcggt
     1921 tccgcttcgg cgccatttac atgccaaacg gtgtttatcc cgggctctgg cacccgacga
     1981 aggtcggtaa gaacttcgat ttcaaccgca cgatgacgcc gctcgagccg tatcgtgagt
     2041 acctgacgac gatcagcaaa ctgaaggcgc ccgaaggtcg caaggacatg ggcggcattc
     2101 acatgggcgc cagcgccgcg ttcttgaacg gcacgggccc gctcacccac aacggtgact
     2161 tcaacctcat cgagtcgaag aagtcgatcg atcagtacat cgccgacaag atcgccggcg
     2221 atacgccgct gcgctccctc gagctcggca cggaagacat gggcacgtcc gccggcgctt
     2281 gcgacggtta tccctgcgtg ttcttcaaca cgatggcctg gtacgacgac agcagcccgt
     2341 tgccggtgtc ggtgaatccg caggccacgt ttgagcgcat gtttggtgac cccggcacgc
     2401 cggagcagcg cctggcgcgc ttgcacgcca agcagagcat gctcgactcc gtcatgcagg
     2461 agacgtcgcg gttgcagaag gtcatcggcg cgaaggacaa cgcaatcctc gacgagtacc
     2521 tgaccaacat tcgccgcgtc gaagagcagc tgcagaagat gcaggcccgc tcgggtgcat
     2581 tgacgaacgc ggccggcggc ccggtcggta tccccgagga cttcaacgag cacatgacgg
     2641 tcacgtacga cttgctgcac ctcgctttcc agggcgacat cacgcgcgtg ttcagcttca
     2701 tgctcggcca cgaaggcagc ggccgcagct acgcgcacgt cggcgtcaaa gagccgcacc
     2761 attcgacgtc gcaccacaag aacacgccgg agagcctcga gcagtacgcg aggatcggtg
     2821 cgtaccatat ggtcaagctg ggcgagttcg ccgagaagct caagaacacg ccggatggcg
     2881 acggctcgct gctcgacagc tccctgatct actttggcgc gggcatgagc aacggcctgc
     2941 tgcacgaccg tcacaacgtg ccggccgcgt tgcttggccg agccaacggt cggcttgcgg
     3001 gcaaccgcca catcgaggcc acggtcgatc agccgacctc ggatctcttg ctcggtatgg
     3061 ccgacgtcgt cggcgccgag gtcgagacga tcggcatcgc gacgggccgc ttgaacttgg
     3121 cgtcagcctc ttagttcgct ttcagtcgga accgacgccg ccgttacaac ggcggcgtct
     3181 ttttttttgc gtggagtgcc gcagtacttg aaaccgggga tcctgaactc ggtgctcgtc
     3241 gagcaagtcc gcaacagcgc ggcgtggctc tggctcgatc cacgctgtgc cgcgctgacg
     3301 gcggtgttcg acgcagccta ttacgagccg ctcgaagagc ctgcatcgct gctgcgggcg
     3361 tttcggctgc gtttggccgc gcattatgcg acggtcgcca cgttcgtgcc gaccgacgtc
     3421 gacgcgcata tccgccatca cacctgggtg gccataacgg atgccgacgt tttttcgcag
     3481 gcgcgtctgt gcgtcgacga ggcagccgcg cgcgacgctc acctcgtctc ggagcgcgtg
     3541 acgcaaggac tgtccgggca cgacggcgaa tggttctccg tgagggcggg ggcgctc
//
LOCUS       DATLEE010000255         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2527790, whole genome shotgun sequence.
ACCESSION   DATLEE010000255 DATLEE010000000
VERSION     DATLEE010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2527790"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VM692_05320"
     CDS             complement(<1..1153)
                     /locus_tag="VM692_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUQ51618.1"
                     /translation="MRWHRRVALLLARTAGLTALLAAAAAADEIVVMGSGAFTAPYND
                     VVPWFEKSTHHELVSVYGASTGGAADSIPTRLARGEPADVIIVSAQALDALIAAGEVE
                     PGSRVDLVLSRIGVAVRAGAPKPDIGSVDALVRAVRASSSFAYSASVSGTYLSTELLP
                     RLGLTDEIAAKGQRIESERVGTVVARGGAELGFQQISELLPIDGIDYVGPLPDEVQRV
                     STFSAGIAVGARSPAAARELIAFLASPALGPVVEKYGLEQIGAEGKDWRVLFNGRDLT
                     GWTPKIRKHTLGDNYANTFRVVDGLLTVAYDGYETFDEQFGHLFFEQPFSHYRLRVEY
                     RFVGAQARDAPAWAARNSGVMVHAQPPATMRRDQDFPISIEVQFLGGLSD"
     gene            1197..2021
                     /locus_tag="VM692_05325"
     CDS             1197..2021
                     /locus_tag="VM692_05325"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HUQ51619.1"
                     /translation="MSRAHYAIALALLLTASTAAAQQVWREYPSIERGWYGRDAPHEQ
                     EPGEFVVGRLMFPQTRTRRLLGAGPGDWRRGGTAWTVDYPEGDRTFARLLKRLSTIDV
                     RLVEQPVNLDDDIDAYYWPFLISGLVGTWDLTDEQAAKLREYLLRGGFLLCDSFYGTV
                     EWEGFVEGIKKVFPDRPIVDLPDTHPIFHAVYDLTNRPQIPTWQHLPYGGYRNGGAVA
                     RWRAIVDDDDRVMVMITFNNDVADGWQRAEEIDYPQEAANLAISLGVNFAVYALSH"
BASE COUNT          341 a          693 c          686 g          320 t
ORIGIN      
        1 cgtcgctcaa gccacccaag aattgcacct cgatcgagat cgggaagtcc tggtcgcgcc
       61 gcatcgtcgc cggcggctgc gcgtgaacca tgacgccgct gttgcgtgcg gcccaggccg
      121 gcgcatcgcg cgcctgcgcg ccgacgaacc gatactcgac gcgcaagcgg tagtgcgaaa
      181 acggttgctc gaagaacagg tggccgaact gctcgtcgaa cgtctcgtag ccgtcgtacg
      241 cgacggtcaa caggccgtcg acgacgcgaa acgtattggc gtagttgtcg cccagcgtgt
      301 gcttccgaat cttcggcgtc caacccgtga ggtcgcggcc gttgaacagc acgcgccagt
      361 ccttgccttc cgcgccgatt tgctcgagcc cgtatttctc gacgacgggg ccgagcgccg
      421 gcgaggcgag gaacgcgatg agctcgcgcg cggctgcggg actgcgcgcg ccgacggcga
      481 tgccggccga gaacgtgctg acgcgctgca cctcgtccgg caacggcccg acgtaatcga
      541 tgccgtcgat cggcaatagc tcgctgatct gctgaaagcc gagctcggcg ccgccgcgcg
      601 cgacgacggt gccgacgcgc tcgctctcga tgcgctggcc tttggccgcg atctcgtcgg
      661 tcagccccaa gcgcggcaac agctccgtcg acaaataggt gccgctgacg ctcgccgagt
      721 aggcgaagga gctcgaggcg cggacggcgc gcacgagcgc gtccacggag ccgatgtcgg
      781 gctttggtgc gccggctcgg accgccacgc cgatgcgcga cagcacgaga tcgacgcgtg
      841 agcccggctc gacctcgccg gcggctatca acgcatcgag cgcttgcgcg gagacgatga
      901 tgacgtcggc gggctcgccg cgcgcgagcc gcgtggggat cgagtccgcg gcgccgcccg
      961 tcgaggcgcc gtagacgctg acgagctcgt ggtgcgtcga cttttcgaac cacgggacga
     1021 cgtcgttata gggcgccgtg aacgcgccgg atcccatgac gacgatctcg tccgcggcgg
     1081 ctgccgccgc cagcagcgcc gtgaggcccg cagtgcgcgc gagcagaagc gcgactcgcc
     1141 gatgccatct catgccgggt cgtaggccct tgggttcgct gttacagtaa tagtgaatga
     1201 gccgagcgca ctacgctatt gccctggcac tgctgctcac cgcaagcacg gcggcggcgc
     1261 aacaggtctg gcgcgagtat cccagcatcg agcgcggctg gtacggccgc gatgcgccgc
     1321 acgagcaaga gcccggtgaa ttcgtcgtcg ggcggctcat gttccctcag acgcgcacgc
     1381 gccggctgct cggcgcgggc cccggcgatt ggcgccgcgg cggcacggcg tggaccgtcg
     1441 actaccccga aggcgaccgc acgttcgcgc ggctcctgaa gcggctgtcg acgatcgacg
     1501 tgcggctcgt cgagcagccc gtgaatctcg acgacgacat cgacgcctac tattggccct
     1561 tcctgatctc ggggctcgtc ggcacctggg atctgacgga cgagcaagcc gcgaaactac
     1621 gcgagtatct gttgcgcggc gggttcctgc tctgcgacag cttctatggc accgtcgagt
     1681 gggaaggctt cgtcgaaggc atcaaaaagg tatttccgga tcgaccgatc gtcgacctgc
     1741 ccgacacgca cccgatcttt cacgccgtct acgacctgac gaatcgcccg cagattccga
     1801 cctggcagca cttgccctac ggcggttacc gcaacggcgg cgccgtcgcg cgctggcgcg
     1861 cgatcgtcga cgacgacgat cgcgtcatgg tcatgatcac gttcaacaac gacgtcgccg
     1921 acggttggca gcgcgccgaa gagatcgact atccgcagga agcggcaaat ctcgcgatca
     1981 gcttgggcgt caacttcgcg gtgtacgcgc tctcgcacta ggccgaaccg tacgccgggc
//
LOCUS       DATLEE010000256         4790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4759536, whole genome shotgun sequence.
ACCESSION   DATLEE010000256 DATLEE010000000
VERSION     DATLEE010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4759536"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VM692_05330"
     CDS             <1..496
                     /locus_tag="VM692_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HUQ51620.1"
                     /translation="INDIVLNEGTPHEQRRPFEIFINSKNLDHYQWIVALTRIMSAVF
                     RKGGDVTFLVDELKAVFDPRGGYWQPGGKFMPSIIAELGHIVEKHLIEIGLLKGPDLS
                     ADQKEFLAKKKAEFEQRASQKDAFASNSFPEGAQLCGKCNTAAAVMLDGCMTCLNCGY
                     SKCG"
     gene            673..2100
                     /gene="hldE"
                     /locus_tag="VM692_05335"
     CDS             673..2100
                     /gene="hldE"
                     /locus_tag="VM692_05335"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HUQ51621.1"
                     /translation="MTLTVPDFSKLRVLVAGDAMLDEYWFGDTARISPEAPVPVVRAR
                     AAEQRPGGAANVALNVAALGARSVLAAVIGKDDRGERLTRALEQRGVRCDLVRSATLP
                     TIHKLRVLARSQQLLRIDAEQSLHSCADDFGALFAKLVRQTDVVVLSDYAKGTLSRVA
                     ELIAAARAASVPVLVDPKGTDFKRYRGAYALTPNRSEFEAVVGRCADEAEFLRKGEEL
                     RAELDVALLLVTRGEQGMTLFEQGAEALTLPTQAREVFDVTGAGDTVIALLAAGVAAG
                     LAAADAAALANLGAGIVVGKIGVATVSRSELQHALHSQGSGGRGLVELPELVALVAEA
                     RARGERVVLTNGCFDILHAGHVGYLEEAKSCGDRLIIAVNDDDSVRRLKGASRPVNSL
                     ADRMAVLAGLASVDWVVPFSEDTPAELIAKVVPDVLVKGGDYRVEQIAGGDTVLKNGG
                     EVRVLGFKPGRSTTALIDALRKTDG"
     gene            complement(2134..3516)
                     /locus_tag="VM692_05340"
     CDS             complement(2134..3516)
                     /locus_tag="VM692_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HUQ51622.1"
                     /translation="MSIKKHLLALALSACVTIPAWAQESLIDVYRRAVENDPAVREAE
                     ATFRANSETKAQTRSLFLPTLNASANLSNQYSDSRGGVELPGGAVVGGDRNITDADAS
                     RWSLQLTQTLFDWGNFAQLRQADKVVARAETTYRAATQDLMLRVATRYFDVLAAEDNL
                     ASAVARRESVSRQLEQSQRRFEVGLIAITDVQQSQAGYDDANAAEIEAQRAVATAYEF
                     LREIIGELVDDLASPTDELPLLSPDPTNAEQWVQTALQQNLALVTSRLATEIALDDID
                     IQRSNRLPTLSLSAGYSEGTNERSSTSYTNGVATQGFPSTTRPDGRQWSLDVRFPIFT
                     GGLNQSRIRQSVYNHQAAEQALERIARQTERQTRDAYLGVISEISRVRALRQSVESNR
                     TALRATEAGFEVGTQTTVDVLRAQELLSQAETTYSRSRYDYIRNTLLLKQAAGTLSEA
                     DIQQVDSWLQ"
     gene            complement(3548..3883)
                     /locus_tag="VM692_05345"
     CDS             complement(3548..3883)
                     /locus_tag="VM692_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HUQ51623.1"
                     /translation="MPGVPELSPTEFANRWPDYAHGDDVVLLDVREHDELAVAAVAGA
                     LHIPMREVPGRLAELDAHKPLVVMCHSGGRSRRVAEYLLGQGFATVFNLKGGIDAWSS
                     QLDSEIPRY"
     gene            complement(3905..4555)
                     /locus_tag="VM692_05350"
     CDS             complement(3905..4555)
                     /locus_tag="VM692_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HUQ51624.1"
                     /translation="MDFELARTQMLGQQLRAWEVLDDRVLRAFGETPRENFVPSDYRD
                     LAFADVEIPLGHGQSMLAPKVEGRFLQALQIEPIDDVLVVGTGRGYLTACIARLAKHV
                     TSIDIFPDLVAAAQSKIAASGIRNVELTATDAFDLSYRARFDAIAVTGSVPHLDQHFT
                     AMLRPQGRLCIVVGRAPAMEARVVTLQPDGTTTTDSLFETVLAPLRNAERPEPFVL"
BASE COUNT          797 a         1512 c         1623 g          858 t
ORIGIN      
        1 gatcaacgac atcgtgttga acgaaggcac gccgcacgag cagcggcggc cgttcgagat
       61 cttcatcaac tcgaagaacc tcgaccacta tcaatggatc gtggcgttga cgcgaatcat
      121 gtcggctgtg ttccgcaagg gcggcgacgt gaccttcctc gtcgacgagc tgaaggcggt
      181 gttcgatccg cgcggcggct attggcagcc gggcggcaag ttcatgccgt cgatcatcgc
      241 cgagctcggg cacatcgtcg aaaagcattt gatcgagatc ggtttgctga aaggtcctga
      301 cctgagcgcc gatcaaaagg agttcctcgc gaagaagaaa gcggagttcg agcagcgcgc
      361 cagtcagaag gacgcgttcg cgagcaacag ctttcccgag ggcgcgcagc tctgcggcaa
      421 gtgcaatacg gctgcggccg tgatgctcga cggctgcatg acgtgcttga actgcggtta
      481 ttcgaagtgc ggttagtcgc gccccccttc gggggctgcc aagggtcgcc ggcttaaaaa
      541 tggtcggcct cgaacgcggg tacggtttca agagttcgta ctagtccgcc gttggtccgc
      601 cccttgagaa atcggggcgg gccctttttc ccttagaatg gcgccattca ccccacgcgg
      661 ctcgaagcac cggtgacgct gacagtccca gacttctcca agctacgagt gctcgttgcc
      721 ggtgacgcaa tgctcgacga gtattggttc ggcgacaccg cgcggatctc gccggaagcg
      781 ccggtgcccg tcgttcgcgc gcgcgccgcg gagcagcggc cgggcggagc ggccaacgtc
      841 gctctgaacg tcgccgcgct cggggcacgc agcgttctgg ccgccgtcat tggcaaagac
      901 gaccgcggcg agcgtctcac gcgcgcgctc gagcagcgcg gcgtccgctg cgatctcgtg
      961 cggtcggcga ccttgccgac gatccacaag ctgcgagtct tggcgcgcag tcagcagctg
     1021 ttgcgcatcg atgccgagca atcgctgcac tcctgcgccg acgacttcgg cgctctgttc
     1081 gccaagctcg tgcgccaaac ggacgtcgtc gtcctatcgg actacgcgaa ggggacgctg
     1141 agccgcgtcg ccgagctcat cgccgccgcg cgcgccgcct ccgttcccgt gctagtcgat
     1201 ccgaagggca cggacttcaa gcgttatcgc ggcgcctatg cgctgacgcc gaaccgcagc
     1261 gagttcgagg ccgtcgtcgg gcgctgcgcc gacgaagccg agttcctacg caagggcgag
     1321 gagctgcgcg ccgagctcga cgtcgcgctg ctgctcgtca cgcgcggcga gcagggaatg
     1381 acgctgttcg agcagggcgc cgaggcgctg acgctgccga cgcaggcgcg agaagtcttc
     1441 gacgtgaccg gcgccggcga taccgtgatc gcgctgctcg cggcgggcgt cgcggccgga
     1501 ctggccgccg cggacgctgc ggcgcttgcg aatctcggcg ccggcatcgt cgtgggcaag
     1561 atcggcgtcg cgaccgtcag ccgctccgag ttacagcacg cgttgcactc gcaaggcagc
     1621 ggcggccgcg gcctcgtcga gctaccggag ctcgtggcac tcgtggcgga agccagagcg
     1681 cgtggcgagc gcgtcgtgct gacgaacggc tgcttcgaca tcctgcacgc aggccatgtc
     1741 ggctatctcg aggaagcgaa gtcgtgcggc gaccggctca tcatcgccgt caacgacgac
     1801 gattctgtcc ggcggctcaa gggagccagt cggcccgtca attcgctggc cgatcgcatg
     1861 gccgtgctcg cggggctcgc gtcggtcgat tgggtcgtgc cgttcagcga agacacgccg
     1921 gccgagctca tcgcgaaagt cgttcccgac gtgctcgtga agggcggcga ctatcgtgtc
     1981 gagcagatcg cgggcggcga cacggtcctg aaaaatggcg gcgaggtgcg cgtgctcggc
     2041 ttcaagcctg ggcggtcgac gaccgcactg atcgacgcgc tgcgcaagac ggacggctag
     2101 cgcggcgttg ccgtgccagc cgtcgtccga gtcctactgc aaccagctgt cgacctgctg
     2161 gatatcggct tccgacaacg tgcccgcagc ttgcttcagc agcagcgtgt tcctgatgta
     2221 gtcgtagcgg ctgcgcgagt acgtcgtctc cgcttggctc agcagctctt gcgcgcgcag
     2281 cacgtcgacg gtcgtctgcg tgccgacttc gaagcccgct tccgtcgcgc gtagcgccgt
     2341 gcggttcgat tcgaccgact gccgcagcgc gcgcacgcgc gagatctccg agatcacgcc
     2401 gaggtacgca tcacgggttt gacgctccgt ctgccgggcg atgcgctcca gcgcctgctc
     2461 ggcggcctga tggttgtaga cggactggcg aatgcgcgac tgattcaacc cgcccgtgaa
     2521 gatcgggaag cgcacgtcga gcgaccactg gcgaccgtct gggcgcgtcg tcgatggaaa
     2581 gccctgcgtc gctacgccgt tggtgtacga cgtcgacgaa cgctcgttcg tgccctcgct
     2641 gtaccctgcc gaaaggctca gcgtcggcaa gcgattcgat cgttggatgt cgatatcgtc
     2701 gagcgcgatc tccgtcgcaa ggcggctcgt gacgagcgcg agattctgct gcaacgccgt
     2761 ctgcacccac tgctcggcat tggtcggatc cggcgacaga agcggcagct cgtcggtcgg
     2821 gctcgcgaga tcgtcgacga gctcaccgat gatctcgcgc aggaactcgt acgcggtcgc
     2881 gaccgcgcgt tgcgcttcga tctcggccgc gttcgcgtcg tcgtagcccg cttgtgactg
     2941 ctgaacgtcg gtgatcgcga tgaggcctac ctcgaagcgg cgttgtgatt gctcgagctg
     3001 gcgcgagacg ctctcgcgcc gcgccacagc cgacgcaagg ttgtcctcgg ccgcgagcac
     3061 atcgaaatag cgcgtggcga ctcgcagcat caagtcctgc gtcgctgcgc ggtacgtcgt
     3121 ctcggcgcgt gcgacgacct tgtcggcctg ccgcagctgc gcgaagttgc cccagtcgaa
     3181 caaggtttgt gtgagctgca agctccagcg gctcgcgtcg gcgtccgtga tgttgcggtc
     3241 gccgccgacg acggcaccgc ccggcagctc gacgccgccg cgcgaatcgg agtattggtt
     3301 gctcaggttc gcgctcgcat tgagcgtcgg caaaaacaaa ctacgcgttt gcgctttcgt
     3361 ctccgagttg gctcgaaacg tcgcttccgc ctcgcggacc gccgggtcgt tctcaaccgc
     3421 gcgtcgatac acgtcgatta ggctttcctg cgcccaagct ggaatggtga cgcacgcgct
     3481 gagagcgagc gccagtaggt gcttcttgat tgacatcgtg gttatccctg tctcttatcg
     3541 tcgttggtta gtagcgcggt atttcggagt ctagctggct cgaccaggcg tcgataccgc
     3601 ccttgagatt aaaaacggtc gcgaagccct gccccagcaa atactcggcg actcggcggc
     3661 tgcggccacc cgagtggcac atcacgacga gcggtttgtg cgcgtcgagc tcggcgaggc
     3721 gcccggggac ctcgcgcatc ggaatgtgta acgcgccggc gacagcggcc accgcgagct
     3781 cgtcgtgctc gcggacgtcg agcaacacga cgtcgtcgcc gtgcgcgtag tcgggccagc
     3841 ggttggcaaa ctcagtgggg cttagctccg ggacgcccgg catgagggtg ctcgggttct
     3901 ctgctcagag aacgaacggc tccggccgct cggcgttacg caacggcgcg agcacggttt
     3961 cgaacaggct gtcggtcgtc gtcgtgccgt ccggttgtag cgtgacgact cgagcctcca
     4021 tggctggcgc gcgtccgacg acgatgcaaa gccgcccctg cggccgaagc attgcagtga
     4081 agtgttgatc caagtgcggc accgatcccg tcaccgcgat ggcgtcgaac cgagcgcggt
     4141 acgaaaggtc gaaggcgtcg gtggccgtga gctcgacgtt acgaatgccg gaggcggcga
     4201 tcttcgactg cgcggctgcg acgagatccg gaaagatgtc gatgctcgtc acgtgcttgg
     4261 cgagccgagc gatgcaggcg gtcaggtagc cgcggcccgt accgacgacg agcacgtcgt
     4321 cgatcggctc gatctgcagc gcttgcagga agcgtccctc gactttgggc gcgagcatgc
     4381 tctggccgtg gcccagcgga atctcgacgt cggcgaacgc gaggtcgcgg tagtcgctcg
     4441 gcacgaaatt ctcgcgcggc gtctcgccga acgcgcgcaa cacgcggtcg tcgagcactt
     4501 cccaagcccg caattgctgg ccgagcattt gggtacgtgc gagctcgaaa tccatgagtg
     4561 ttgttgtcgc gggcgggggc tacgtggcga acgcggtagg gtatgaagtt tattcatgcg
     4621 atacaaggcg gtatgtgaag aattatggtc aatttggagc gacgctccaa gctgcttgct
     4681 gtatcgtgaa acgcaaggaa aagcagccgt tttgcgacgc cggcccgatg cctaccgagg
     4741 ccgcgaaggt tgtgagacga gctgggcgcg cgataacaac aataagaggc
//
LOCUS       DATLEE010000257         3564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6855318, whole genome shotgun sequence.
ACCESSION   DATLEE010000257 DATLEE010000000
VERSION     DATLEE010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6855318"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VM692_05355"
     CDS             complement(<1..1059)
                     /locus_tag="VM692_05355"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUQ51625.1"
                     /translation="MPTRAPPNSSAASGYAPIAQLVKMLLPSARSVAIYDPHAELLWC
                     SDGFERPDLRALLEHQRASETVANRGRVETTSDGIPVFISGLRGADTRPLGSLVIELG
                     GSTSRNTPSMVVSMLRPLLDCLEGLLDLEQHSAAADRSAGLELLLSVDEHGREDTSAL
                     QELLRHCARDLNCVAGALVVPDKNLEVSFSSGAAPQSAQLLDRTQKHLLAWARLNNRP
                     MVVNRAAAGAPYKILSCPLRDPRGGVLGLLALFRAIDADDFEPSDVRILEFVSRKAVA
                     ILSSEFDALTGLPNRFIFEHRAQRALERAAAALLYIDIDKLAAINEGFGLSAGDEVIH
                     RVGTLIQRCAGAEALVSRI"
     gene            complement(1164..1529)
                     /locus_tag="VM692_05360"
     CDS             complement(1164..1529)
                     /locus_tag="VM692_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51626.1"
                     /translation="MSPFVQRFGEAVQALIGDGPVKQRLAHAYARHLADVADADLPAP
                     LRREFGELQAAMTRISPVGSETRVRASVQKMSPHEAAGHAGTIVKLYVELMNGHERAE
                     PLKVVPQPRKAPRFLTGRS"
     gene            complement(1657..3456)
                     /locus_tag="VM692_05365"
     CDS             complement(1657..3456)
                     /locus_tag="VM692_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUQ51627.1"
                     /translation="MSQNEQYQWLFRRSPALVASLNEEGFFVDASDAWLRRFGYAREE
                     ITNMRPQDLASPEYAKRIVEEYLPVLRRTGRLAGVPVDLVTRNNERVDCLANAIVERD
                     ETGGYLRTIAVYTEVGEQARLEQHYRELYRATPAMLHTVDSHGRITNVSDRWLEKLGY
                     SREEVLGRVITEFHGKESQATLGGGRLEDVIATGELENEPRTYVTKSGEVLEVEVSAT
                     ADRDPSGAVVALLVASKDVTERNRAERQLRAAFEENARLRDELERERDYLREEVQVSM
                     NFGRIIGESPALKQMLVRLDAVAQTSASVLIEGESGVGKELVAHVIHARSPRSSGPLV
                     KVNCASIPHELFESEFFGHVKGAFTGAHRDRVGRFQLADGGTIFLDEVGEIPLDLQSK
                     LLRVLQESEYERVGDDRTHTVDVRVVAATNRDLEQAVADGRFREDLFYRLSVFPIEVP
                     PLRARGDDVIQIASHFLERTCQEFGHRPLTLSKQQAALLKRYDWPGNVRELKNVIERA
                     VILSRGKVLRLDLAMSDILNAPAESAPEAEVARTQLLTETEMRALERKNTQLALEMAQ
                     WRVSGPSGAAKLLGVKPTTLTDRIKKLKLRKPA"
BASE COUNT          607 a         1154 c         1143 g          660 t
ORIGIN      
        1 gatgcggctg acgagcgctt ctgcgcctgc gcagcgctga atcagcgtgc cgacgcgatg
       61 aatcacttcg tctccagcgc tcaagccgaa gccctcgttg atggctgcca gtttatcgat
      121 gtcgatgtag agcagcgccg ccgcggcgcg ctcgagggcg cgctgcgcgc ggtgctcgaa
      181 gatgaagcgg ttcggtagac ccgtcagcgc gtcgaactcg ctgctcaaaa tagcgacggc
      241 cttgcggctg acgaactcga ggatgcgcac gtcgctcggc tcgaaatcat cggcgtcgat
      301 cgcgcgaaat agcgccaaca gccccaacac gccgccgcgc gggtcgcgca gcggacagga
      361 aagaattttg taaggcgctc cggccgcggc gcgattgacg accatcggcc ggttgttgag
      421 ccgcgcccac gcgagcagat gcttttgcgt gcggtccaaa agctgcgccg actgcggcgc
      481 tgcaccgctg ctgaacgata cctcgagatt cttgtccggc actaccagcg ccccggccac
      541 gcaattcaga tcgcgcgcgc agtggcgcaa cagctcttgc agggcgctcg tgtcctcgcg
      601 cccgtgctcg tcgacggaca acagcaactc gagcccggcg ctgcgatcgg cggccgccga
      661 gtgttgctcg agatccagca atccctcgag gcaatcgagc agcggccgca gcatgctgac
      721 gaccatcgac ggcgtgttgc gcgaggtgct gccgccgagc tctatgacga gcgagccgag
      781 cggccgagta tcggcgccgc gcaggcccga gatgaacacg ggaatgccgt cgctcgtggt
      841 ttcaacgcgg cctctgttgg cgactgtctc gctggcgcgc tggtgctcga gcagcgcgcg
      901 cagatccggg cgctcgaagc cgtccgagca ccaaaggagc tcggcgtgcg ggtcgtagat
      961 cgcgacgctg cgggcgctcg gcaacagcat tttgacgagc tgcgcgatcg gcgcgtagcc
     1021 gctcgcagcc gagctgttcg gtggagcgcg ggtcggcacg caatatgtcc agtcacgagg
     1081 cggacttgcc tcgcgcaagc atgaatagag gcgtccgcca aaacgttgat ggcagtcgca
     1141 actactgccg tgcagggatt tttctaggat cggccagtca ggaaacgagg cgccttgcgc
     1201 ggttgtggga cgaccttcaa aggctcagca cgctcgtggc cgttcatgag ctcgacatag
     1261 agcttgacga tcgtgccggc gtgcccggca gcctcgtgcg gtgacatctt ctggacgctt
     1321 gctcggacgc gtgtctcgct gcccacgggg ctgatgcgcg tcatggccgc ttggagctcg
     1381 ccgaattcac ggcgcagggg ggccgggagg tccgcgtcgg cgacgtcagc caagtgccga
     1441 gcgtaggcgt gcgcgagccg ctgtttcacg ggtccgtcgc cgatcaaagc ttggacagcc
     1501 tcgccgaaac gctgcacgaa aggagacacg acggttgagc cttgctgttg gtgtttggtt
     1561 ggcccgccgg cgggtttcat gccgctggct tcacgtagtc atataccaga gtgccgcaaa
     1621 tctcggcaag cccatgatta gtagccgaag ttggggtcac gccggtttgc gaagcttcag
     1681 ctttttgatg cggtcggtca gcgtcgtcgg tttgacgccg agcagctttg cggcgccgct
     1741 tgggcccgat acccgccact gcgccatctc gagcgcgagc tgcgtgttct tgcgctcgag
     1801 cgcacgcatc tcggtctcgg tcagcagctg agtcctcgcg acctccgctt cgggcgccga
     1861 ctctgccggc gcgttcaaga tgtcgctcat cgcaagatcg agccgcagca ccttgccgcg
     1921 cgacaggatt acggcgcgct cgatgacgtt cttcagctcg cgcacgttgc cgggccagtc
     1981 gtagcgcttt agtagcgctg cttgctgctt cgacagcgtc aacggccggt ggccgaactc
     2041 ctgacacgtt ctctcgagga agtggcttgc gatttgaata acgtcgtcgc cgcgcgcgcg
     2101 cagcggcggc acttcgatcg ggaagacgct caagcgatag aacaagtcct cgcgaaaccg
     2161 gccgtccgca acggcttgct cgagatcgcg attggtcgcg gcgacgacgc gcacgtcgac
     2221 cgtatgagtg cgatcgtcac cgactcgctc gtactcgctc tcctgcagca ctcgcaacag
     2281 cttgctctgc aagtcgagcg ggatctcgcc gacttcgtcg agaaagatcg tgccgccatc
     2341 ggccagctgg aaacgcccga ctcgatcgcg gtgcgcgccc gtgaatgcgc ctttcacgtg
     2401 accgaaaaac tcactctcga agagctcgtg cggaatcgag gcgcaattca ctttgacgag
     2461 cggcccgctg ctacgcgggc tgcgcgcatg gattacgtgc gccacgagct ccttcccgac
     2521 gccggactcg ccttcgatga gcacgctcgc cgacgtctgc gcgacggcgt cgaggcgcac
     2581 gagcatctgc ttcagcgcgg ggctctcgcc gatgatgcgg ccgaaattca tcgacacttg
     2641 cacttcctcg cgcagatagt cgcgctcgcg ctcgagctcg tcgcgcaagc gcgcgttctc
     2701 ctcgaaggcg gcgcgcagct ggcgctcggc ccgattgcgc tcggtgacgt ccttcgacgc
     2761 cacgagtaac gcgacgacgg cgccgctcgg atcgcgatcg gccgtcgcgg acacttcgac
     2821 ctctagcacc tcgccggact tcgtcacgta cgtgcgcggc tcgttctcga gctcgccggt
     2881 cgcgatcacg tcctccaacc ggccgccccc gagggtcgcc tgcgactcct tgccgtggaa
     2941 ctcggtgatg acgcggccga gaacctcctc gcgactgtag cccaacttct cgagccagcg
     3001 gtcgctgacg ttggtgatac ggccgtggct gtcgaccgtg tgcagcatcg ccggcgtggc
     3061 gcgatagagc tcacgataat gctgctcgag ccgcgcctgc tcaccgactt ccgtgtagac
     3121 ggcgatcgtg cgcaggtagc cgcccgtctc gtcgcgttcg acgatcgcgt tagccaggca
     3181 atcgacgcgc tcgttgttgc gtgtgacgag atcgacggga actccggcca agcgtccggt
     3241 gcgacgcaac acgggcaggt attcctcgac gatgcgcttc gcgtattcgg ggctcgccag
     3301 gtcttgcggc cgcatgttcg tgatctcttc gcgcgcgtaa ccgaaccgtc gtagccatgc
     3361 gtcgctcgca tcgacgaaga agccctcctc gttcaaagag gcgacgaggg cggggctgcg
     3421 gcggaaaagc cactggtatt gctcgttctg cgacataact cgaccacctt acgatttttc
     3481 gtggctccgc gcaacgaaaa gccgtgattt acgaaatatc gtaaaacgta aaaccagcca
     3541 acccattgat ttatagacta tttg
//
LOCUS       DATLEE010000258         2552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5511406, whole genome shotgun sequence.
ACCESSION   DATLEE010000258 DATLEE010000000
VERSION     DATLEE010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5511406"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VM692_05370"
     CDS             <1..966
                     /locus_tag="VM692_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51628.1"
                     /translation="GNAGPDYNGGVRAGDNLFTSSIVALDARTGKYRWHFQQVHHDIW
                     DYDGPSPVVLFDLDLNGTLRKGLAQPSKTGWVYILDRTDGTPLVGIDEQPVPQEPRQA
                     TAATQPVPRGDAFVPQHLEIAPEGYPLVNGGKIFTPYWTDYLIAKPGLLGGANWPPSG
                     YDPGTGYLYVCAGDAASSFSAAETGTERPPAGQYYVGGAFGTNPLPAYGTFTALDVRT
                     NKIVWQQHWADRCYSGATTTAGGLVFVGRNDGRLTALDSANGKKLWEFQTGAGMNSTV
                     SVFEHAGKEYVVAYSAGNYFAASAKGDSVWLFALDGTLDPVAPGR"
     gene            complement(1067..1552)
                     /locus_tag="VM692_05375"
     CDS             complement(1067..1552)
                     /locus_tag="VM692_05375"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009022517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HUQ51629.1"
                     /translation="MQIHVLTTGGTIDKVYFDANSEFQVGSSMIAELLKEAHVTAKVT
                     IEPVLAKDSLDLTDADRQLIRERAIACPVERILITHGTDTMVETGRALAGVAAKTIVL
                     TGSMQPARFRSTDAVFNVGFALGALQSLPPGVYIAINGRIFDPQHVRKNREQQRFEEA
                     D"
     gene            1647..>2552
                     /locus_tag="VM692_05380"
     CDS             1647..>2552
                     /locus_tag="VM692_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51630.1"
                     /translation="MAATVVVLAAGADAQTIRRAPAFADADLVAAPTANWATNGGDWY
                     NRRFSPLTAIDRNNVANLKGVWRTHLRGSGLGAQYSGEAQPLVYDGVIYIVTGANDVF
                     AVDVDDGSILWEYRANLDAANNAVCCGWTSRGVGLGDGKVYVGQLDGKLVALDQRTGK
                     VAWSVQAERWQEGFTITAAPLYFDGLIYTGFAGGERGIRGRVKAFDARDGKLAWTFYT
                     IPGPGDVGHDTWPQDNEIWMDGGAPVWNTPAVDPELGLIYFATGNPGPDYNGSIRSGD
                     NLFSTSIVAVDAKTGAYRWHFQQVHH"
BASE COUNT          427 a          860 c          845 g          420 t
ORIGIN      
        1 ggtaacgccg gtcccgacta caacggcggg gtacgcgcgg gcgacaactt gttcacgtcg
       61 tcgatcgtcg cgctcgatgc acgcacgggc aaataccgct ggcacttcca gcaagtccat
      121 cacgacatct gggattacga cggcccgagc cctgtcgtgc tgttcgatct cgacttgaat
      181 gggaccctgc gcaaagggct cgcgcagccg agcaagacgg gctgggttta catcctcgat
      241 cgcacggacg gcacgccgct cgtcggcatc gacgagcagc ccgtgccgca agagccgcgc
      301 caggcgacgg ccgcgacgca acccgtgccg cgcggcgacg ccttcgtgcc gcagcacctc
      361 gagatcgcgc ccgagggcta cccgctcgtg aacggcggaa aaatattcac gccgtactgg
      421 accgactacc tcattgctaa gcccggcctg ttgggcggcg cgaattggcc gccgagcggc
      481 tacgaccccg ggaccggcta tctctacgtc tgcgccggcg acgccgcgag ctcgttctcc
      541 gcggcggaga ccggcaccga gcgcccgccg gccggccagt actacgtcgg cggcgcgttc
      601 ggaacgaatc cgctgccggc gtacggcacg ttcacggcgc tcgatgtgcg cacgaacaaa
      661 atcgtctggc agcagcattg ggcagaccgc tgctacagcg gcgcgacgac gaccgcgggc
      721 gggctcgtgt tcgtcggccg caatgacgga cggctcacgg cgctcgattc cgcgaacggc
      781 aagaagctct gggagtttca gacgggcgcc ggcatgaatt cgacggtcag cgtcttcgag
      841 cacgccggta aggaatacgt cgtcgcctac tcggccggca attacttcgc ggcctcggcg
      901 aaaggcgaca gcgtgtggct gttcgcgctc gacggcacgc tcgatcccgt cgcaccgggc
      961 aggtaaatgc gaccccgatg agcgccaacc gttcaagcag cctgatcgtc atagcgctgc
     1021 tgctggacgc tccacgacgc tgctcgcagc gatgcagcgg cggccgctag tcggcctcct
     1081 cgaagcgctg ttgctcgcga ttcttacgca cgtgctgcgg atcgaagatc cggccgttga
     1141 tcgcgatgta gacgcccggc ggcaacgact gcaatgcgcc gagcgcgaag ccgacgttga
     1201 agactgcgtc cgtgctgcga aagcgcgcgg gctgcatcga gcccgtcagc acgatcgtct
     1261 tggccgccac gccggcgagc gcgcggcccg tttcgaccat cgtgtcggtg ccgtgcgtga
     1321 tcaggatgcg ctcgacgggg catgcgatcg cgcgctcgcg gatcagctgc cggtccgcgt
     1381 cggtcaggtc gaggctgtcc ttcgcgagca cgggctcgat cgtgaccttc gcggtcacat
     1441 gagcctcctt caatagctcc gcgatcatcg acgatccgac ttggaactcg ctgttggcgt
     1501 cgaagtagac cttgtcgatc gtgccgcccg tggtcagcac gtgaatctgc attggcggcc
     1561 gcgcggagga catcggcaaa ttctggcaca atcgccgcga cttcttcgcg gtcgggaggt
     1621 gtgctctgag gacttcgacg ctactgttgg ccgcgactgt cgtcgtgctc gcggctggcg
     1681 cagacgcaca aacgattcgc cgcgcgcctg cgttcgcgga cgcggacctc gtcgcagcac
     1741 ctacggcgaa ttgggccacg aacggcggcg attggtacaa ccgccgcttc tcgccgttga
     1801 cggcgatcga tcgcaacaac gtcgcaaatc ttaaaggcgt ctggcgcacg cacttgcgcg
     1861 gctcgggcct gggcgcgcag tactccggcg aagcgcagcc gctcgtctac gacggtgtca
     1921 tctacatcgt cacgggcgcg aacgacgtgt tcgccgtcga cgtcgacgat ggctcgatcc
     1981 tctgggagta ccgcgcgaac ctcgacgcgg cgaacaacgc cgtctgctgc ggctggacga
     2041 gccgcggcgt ggggctcggc gacggcaagg tctacgtcgg ccagctcgac ggcaagctcg
     2101 tcgcgctcga tcaacgcacg ggcaaggtcg cgtggtcggt gcaggccgag cgctggcagg
     2161 aaggcttcac gatcacggcc gcgccgctct acttcgacgg gctcatttat acgggcttcg
     2221 ccggcggtga gcgcggcatc cgcggccgcg tcaaagcgtt cgacgcgcgc gacggcaagc
     2281 tcgcgtggac gttctacacg atcccggggc ccggtgacgt cgggcacgac acgtggccgc
     2341 aggacaacga gatctggatg gacggcggcg cgcccgtttg gaacacgccg gctgtcgatc
     2401 cggagctcgg cctcatctat ttcgcgacgg gcaatcccgg ccccgactac aacggctcga
     2461 tccgcagcgg cgacaatttg ttctcgacct cgatcgtcgc cgtcgacgcc aagaccggcg
     2521 cctaccgctg gcactttcag caggtgcatc ac
//
LOCUS       DATLEE010000259         3889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2529454, whole genome shotgun sequence.
ACCESSION   DATLEE010000259 DATLEE010000000
VERSION     DATLEE010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2529454"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VM692_05385"
     CDS             complement(<1..306)
                     /locus_tag="VM692_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HUQ51631.1"
                     /translation="MTRSSLHRCAASSLAAIAAAALAACAPSPEELAAEHGATIGKYC
                     TDCHSQAEQEAGLVLENPDLANPAAHRAKWEAVIHKLSAGLMPPPGEPQPSDEAVASL
                     "
     gene            complement(303..1802)
                     /locus_tag="VM692_05390"
     CDS             complement(303..1802)
                     /locus_tag="VM692_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ51632.1"
                     /translation="MRTLLTTGALASLLILSSGAWATPELDAKLLQAAQAADPAQVRA
                     LLAAGADANARGGDGSTPLLYAAHFGDTASVLALLGAKADPDLTNRYGVAAMHEAALR
                     ADVALLRTLIDAGATVDLALPQGETPLMLASRTSGIDAVRLLIGRGANVNVVEQWQGQ
                     TPLMYAAAQDRGEVAAALIAAGADVNARTPVNDLPERESRVRYYVEIPVAGFTPVMFA
                     ARSGANSALHALIAGKADLDAKTPEGFTPIVIALDNLHFDSAKVLVEGGANADEGGLL
                     GVVEARNRVNYVGEYQTPTGAELSLDLLALMISKGADLMGRMPQPLLDRDARFVPPPP
                     KITDVALIRAARSADIGALRVLLDGGADPSMRDDKRGGITPLVAVLTGPELPSLIEAE
                     RRPLEEEAIAAVDFLLDRGADPAIGNDAGSTALHVAALRAYPGVIKRMAERGLDLNVA
                     DREGFTPLDYAVGNLPRGVRARPPAENAAAAALRELGARGKEDPRTAAR"
     gene            1907..2815
                     /locus_tag="VM692_05395"
     CDS             1907..2815
                     /locus_tag="VM692_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HUQ51633.1"
                     /translation="MHLRHPLPAAALAGLLALGCTPGSPPLEWNDAVVAEIEAWRAQH
                     EESYTRNWATIEGLHFLKPGTQSAGSAPDNDVVVAASLPERLGSFTVAGDEVTFVPAP
                     EAPVTINGTPATSAKTLLDDGAEEPDVIEANGASVVVHRSGARPSLRVRDPNGERARA
                     FRGFEWFPIGRDYHVLGRFIRDATPRTLPVVNTFGDVDTYETEGVVEFTLNGELLRLR
                     PFTTEPGRFYFVFNDASSGVETYEAARFLYSDLRKDGTTILDFNEAYNPPCSFNPFTT
                     CPIPLPENRLPIKVLAGEQKYRGSEP"
     gene            complement(2885..3517)
                     /locus_tag="VM692_05400"
     CDS             complement(2885..3517)
                     /locus_tag="VM692_05400"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HUQ51634.1"
                     /translation="MDIRNVNESQEDEIYVIDGFLRDPTVLRRHALAAEYPVPLETPW
                     YPGRNSQRAYPIAGLDEAIGEITGRAVTPLPDSAHAKFRLCLAGELGKGGVHIDNCHW
                     TGVYFLTLDEHAEGGTDFFRHRPSGTLRAPVYPEDWHHWPFDNVSKLWNEVIHPHTND
                     ATKWELVRRVPMKFNRLVLFRPWQWHNAGASFGEAAENGRLIYLLSYNSP"
     gene            3641..>3889
                     /locus_tag="VM692_05405"
     CDS             3641..>3889
                     /locus_tag="VM692_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51635.1"
                     /translation="MNRSLSVTAALCVLLIALQPSQSNAQSRWQDDAPGRTQRIEVAR
                     LPAPFATPSAREFPAVVAKPGDASLKLPPGFKIDVFTRD"
BASE COUNT          641 a         1357 c         1267 g          624 t
ORIGIN      
        1 gaggctcgcg acggcttcgt cgctcggctg cggctcgccg ggcggcggca tgagccccgc
       61 gctcagcttg tggatcacgg cttcccattt cgcgcggtgc gcagccggat tcgcgagatc
      121 cggattctcg agcacgaggc cagcctcttg ctccgcttgg ctgtggcaat ccgtgcagta
      181 cttgccgatc gtcgcgccgt gctcggcggc cagctcttcc ggcgacggcg cgcaagcggc
      241 aagcgctgcg gcggcgatcg ccgcgagcga cgacgcggcg cagcggtgca aggagcttct
      301 cgtcatcgcg ccgccgtcct cggatcttcc ttgccgcgtg cgccgagctc gcgtagcgcg
      361 gcggccgcgg cgttctcggc cggcggtcgg gcccgcacgc cgcgcggcaa attgccgacg
      421 gcgtagtcga gcggcgtgaa cccttcgcga tccgcgacgt tcaagtccaa gccgcgctcc
      481 gccatacgct tgatgacacc gggatacgcg cgcaacgcag ccacgtgcag cgccgtgctg
      541 ccggcgtcgt taccgatggc gggatccgcc ccacggtcca acaggaagtc gaccgcagcg
      601 atagcctcct cctcgagcgg gcgccgctcg gcctcgatca gcgacggcag ctcgggcccc
      661 gtcagcacgg cgaccaacgg cgtgatgccg ccgcgcttgt cgtcgcgcat cgaaggatct
      721 gcgccgccgt cgagcagcac gcgcagcgcg ccgatgtcgg ccgaccgcgc ggcgcggatc
      781 agcgccacgt cggtgatctt cggcggcggc ggcacgaagc gcgcatcgcg atcgagcaac
      841 ggctgcggca tgcggcccat gagatcggcg cccttcgaaa tcatcaatgc gagcaggtcg
      901 agcgacagct cggcgccggt cggcgtctga tattcaccga cgtagttcac gcgattacgg
      961 gcctcgacca cgcccaacag gccgccctcg tcggcgttcg cgccgccttc gacgagcacc
     1021 ttcgccgaat cgaaatgcag gttgtcgagc gcaatgacga tcggcgtgaa gccttcgggc
     1081 gtcttcgcgt cgaggtcggc cttacccgcg atcagcgcgt gcaacgccga gttggccccg
     1141 ctgcgcgctg cgaacatcac gggcgtgaaa cccgccaccg gaatttcgac gtaatagcga
     1201 acgcgcgact cgcgctcggg cagatcgttg accggagtcc gcgcgttgac gtcggcaccg
     1261 gccgcaatca acgccgccgc tacttcgccg cgatcctgag ccgctgcgta catcaacggc
     1321 gtttggccct gccattgctc gacgacgttg acgttggcgc cgcggccgat cagcagccgg
     1381 acggcgtcga tgccgctcgt ccgcgacgcc aacatcagcg gcgtctcacc ttgcggcaac
     1441 gcaaggtcga cggtcgcacc ggcgtcgatc agagtccgca acagcgctac atccgcgcgc
     1501 aacgcggcct cgtgcattgc cgcgacacca taacggttcg tgagatcggg atccgccttc
     1561 gcgccgagca gcgccagcac gctcgccgtg tcgccgaaat gcgcggcgta gaggagcggc
     1621 gtcgagccgt cgccgccacg cgcattcgcg tcggcacccg ctgcgagcag cgcgcgcacc
     1681 tgcgcgggat cggccgcctg cgcggcctgc agcagcttcg catcgagctc gggcgtcgcc
     1741 caggcgccgg acgacaatat aaggagcgac gccaaggcgc cggttgtgag caacgttcgc
     1801 acgccgtccc ccagtcgaaa tgtcggtgtg gctgacattc cgagcgtatt gataatttga
     1861 caaatcgtca ataacggctt gtacgctcga ttgctatgct tcggccatgc acctgcgtca
     1921 cccgctaccc gcggccgctt tggccggcct cctcgcgctc ggctgcacgc ccggctcgcc
     1981 gccgctcgag tggaacgacg ccgtcgtcgc ggagatcgag gcgtggcgcg cgcagcacga
     2041 ggagagctac acacgcaact gggcgacgat cgaggggctg cacttcctca agcccggcac
     2101 tcaaagcgcc ggcagcgcgc ccgacaacga cgtggtcgtg gcggcctcgc tgcccgagcg
     2161 tctcggcagt ttcacggtcg cgggcgacga ggtgaccttc gtgccggcgc cggaagcgcc
     2221 ggtcacgatc aacggcacac cggcaacgtc ggccaagaca ctgctcgacg acggcgccga
     2281 agagcccgac gtgatagagg ccaacggtgc gagcgtcgtc gtacaccgca gcggcgcacg
     2341 tccgtcgctg cgcgtccgcg atccgaacgg cgagcgcgcg cgtgcctttc gcggcttcga
     2401 atggttcccg atcggccgcg actatcacgt cctcgggcgc ttcattcgcg atgcgacgcc
     2461 gcgcacgttg cccgtcgtca acacgttcgg cgacgtcgac acttacgaga cggaaggcgt
     2521 cgtcgaattc actctgaacg gcgagctgtt gcgtctgcgg ccgttcacga ccgagcccgg
     2581 ccgcttctac ttcgtgttca acgacgcatc gagcggcgtc gagacctacg aggccgcgcg
     2641 cttcctgtat tccgacttga ggaaagacgg cacgacgatt ctcgacttca acgaggcgta
     2701 caacccgccg tgctcgttca atccgttcac gacgtgcccg atcccgttac cggagaaccg
     2761 cctgccgatc aaagtgctcg ccggcgagca gaaatatcgc ggcagcgagc cgtaggcggc
     2821 ccagttccat tacgcggcta aacgaagcgc ggcgatcaga acactgcgat gcgcagctcg
     2881 gttgtcacgg cgagttgtac gacagcaaat agatcagccg cccgttctcg gccgcctcgc
     2941 cgaagctggc accggcgttg tgccattgcc acgggcgaaa cagcacgagc cgattgaatt
     3001 tcatcggcac gcggcgaacg agctcccact tcgtcgcatc attcgtgtgc ggatgaatga
     3061 cctcgttcca tagcttgctg acgttatcga acggccagtg gtgccagtct tcggggtaga
     3121 ccggcgcgcg cagcgtgccg ctcggtcgat ggcgaaaaaa atccgtgccg ccctcggcat
     3181 gctcgtcgag cgtcagaaaa tacacgcccg tccagtgaca gttgtcgatg tggacgccgc
     3241 ctttgccgag ctcgccggcc aggcacagcc gaaacttcgc atgggcgctg tcgggcagcg
     3301 gcgtgacggc gcgccccgtg atctcaccga tggcctcgtc gagccccgcg atcgggtacg
     3361 cgcgctgcga gtttcgtccc gggtaccacg gtgtctcgag cggcaccgga tactcggccg
     3421 ccaacgcgtg ccgacgcagc accgtcggat cgcgcaggaa cccgtcgatc acatagatct
     3481 cgtcctcctg cgactcgttg acgtttcgga tgtccaccgc cgaagaccac cctcgggccg
     3541 accgcgcaac gcggctagtg tcgcaccgcg gcaggctgat gcgaacgctg ccccgcgtgt
     3601 agtatgaggc gtctgcgaca cgatgatgga ggggaatgcc atgaatcgct cgttgtcagt
     3661 gacggccgcg ttgtgtgtgc tgctgatcgc gctacagccc agtcagtcaa acgcgcagtc
     3721 tcgctggcaa gacgacgccc cgggacgcac gcagcgcatc gaagtcgcgc ggttgcccgc
     3781 gccgttcgcg acgccctcgg cgcgcgagtt tccggccgtc gtggcgaagc ccggcgacgc
     3841 gtcgctgaag ctgccgccgg gattcaagat cgacgtcttc acgcgcgac
//
LOCUS       DATLEE010000260         2893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2531676, whole genome shotgun sequence.
ACCESSION   DATLEE010000260 DATLEE010000000
VERSION     DATLEE010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531676"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1070)
                     /locus_tag="VM692_05410"
     CDS             complement(105..1070)
                     /locus_tag="VM692_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51636.1"
                     /translation="MRRSALTAALTLALAGTATAQTCPYQNLLPEFAQLVAATGDLAP
                     PARAAAFAERFAARHPDFYGEQPFGSPGQLIENAARLFDPQRAPTYDGSRAITLEDIL
                     ATARTITADYARIEATFRAAFPDFRCDTRITFGISLYLFDGNQAVDALGKSQLRFGID
                     TIALLHSQRELSAFFHHELFHMYQAQVLGAAVPPDATQPVWWALWNEGLATYVSGQLN
                     PTLTAAEIFWVPRDLEAQLQPRLAEAARLMLVELDGHDGYSRWFEGGSSRPGLPPRSG
                     YYLGYRMAKQLDRGDLAALARMPPDRVQREARAFLESLARDAARR"
     gene            complement(1093..1977)
                     /locus_tag="VM692_05415"
     CDS             complement(1093..1977)
                     /locus_tag="VM692_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51637.1"
                     /translation="MQTNWLAVGLLTGVAMTAGRTASAAEACDRECLRSTMTTFLHAL
                     LAHDASKVPVAGTVRVTEDAVEKPLKNLGLLSTVTRLRGYRQDILDERAGVAGAAVVV
                     EEVGAPVLLVVRLKVVDEQITEIETVATRGRTEGLIFNIDGLSAPSEQMNYAPRPGQL
                     AARQAAIDAAMHYPTGLNAAKTFAAVDAPFAPDAYRYENGQVMAGPDCKFAPGCQNIL
                     TQSLAIFERLGDVATRVIAVDERMGIVWLRMAWGVRQEGGDQLTVWEAFKVYDGQIHA
                     VEAFMKILPFEQRSGGWN"
     gene            complement(1989..2273)
                     /locus_tag="VM692_05420"
     CDS             complement(1989..2273)
                     /locus_tag="VM692_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HUQ51638.1"
                     /translation="MTAKSNRIIVTVIGSDTVGIIANVSAVLAEARANIIDISQTTVR
                     EFFAMIMLADLDKATVSFDELKRKLDAKGLEMGLKIDAQHEDVFKFMHRV"
     gene            complement(2270..>2893)
                     /locus_tag="VM692_05425"
     CDS             complement(2270..>2893)
                     /locus_tag="VM692_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51639.1"
                     /translation="NLVPKSLLEAVEAGKVAMADVPSHGWFPPPVTLTAAGQAASAAL
                     RDLPPERNPRLSCQITSVLFDWVFDGTINRITQGDDVIKMEYGRGLERTVHMDLAAHP
                     SNVAPSRAGHSIGRWDGDTLVVDTVAFEPGSLAGTVAHSDKLHLVERFTLDPATLALR
                     REYVATDPLYFVDEYVSGDTVLPADAPFAVDACKELAFEYQPGDARQ"
BASE COUNT          465 a          956 c          983 g          489 t
ORIGIN      
        1 accgcattcg ccatttcatg cacggcctcc agacggctcg agggctcacc gcatggtagc
       61 gcagacgaca gcgcgcgaac gagcgtgccg tcagtcaagg cacgtcaacg ccttgcggca
      121 tctcgggcca gtgactcgag aaatgcgcgc gcttcgcgct ggacccggtc gggcggcatg
      181 cgggccagcg cagcaagatc gccgcggtcg agctgcttgg ccattcgata gccgaggtag
      241 tagccgctgc gcggcggcaa gccgggccgg ctcgagccgc cctcgaacca acggctgtaa
      301 ccgtcgtgac cgtcgagctc gacgagcatc agccgcgcgg cctccgcaag cctcggctgc
      361 agctgcgcct cgaggtcgcg cggtacccag aaaatttcgg cagccgtcag cgtcggattg
      421 agttgcccgc tgacgtaggt cgcaaggccc tcgttccata gcgcccacca cacgggctgc
      481 gtcgcgtcgg gcggcacggc cgcgcccagc acttgtgcct gatacatgtg aaacagctcg
      541 tggtggaaga acgccgacag ctcgcgctgc gaatgcagca gcgcgatcgt gtcgatgccg
      601 aagcgcagct gcgatttgcc caacgcgtcg acggcttgat tgccgtcgaa caagtagagc
      661 gagatgccga acgtgatccg agtgtcgcag cgaaagtcgg gaaaggcggc gcgaaatgtc
      721 gcctcgatgc gcgcgtagtc cgcagtgatc gtgcgcgccg tggccaagat gtcctcgagc
      781 gtgattgccc gagagccgtc ataggtcggc gcgcgttgcg ggtcgaagag ccgcgcggcg
      841 ttctcgatca gctgcccggg tgagccgaac ggctgctcgc cgtagaaatc gggatgccgc
      901 gcggcaaagc gctcggcgaa agccgccgcg cgcgccggcg gggcgaggtc gccggtggcg
      961 gcgacgagct gcgcgaactc cggcaacagg ttttggtacg ggcacgtctg cgccgtcgcc
     1021 gtgccggcga gcgccaaagt cagtgccgcg gtgagggccg accgtcgcat cggcgcccga
     1081 gcctacgcct gcctagttcc agccgcccga tcgctgctcg aacggcagga ttttcatgaa
     1141 cgcttcgacg gcgtggatct ggccgtcata gaccttgaac gcttcccaga cggtcagctg
     1201 gtcgccgcct tcctgccgca cgccccacgc catgcgcagc cagacgatgc ccattcgctc
     1261 gtcgacggcg atcacgcgcg tggcgacgtc gccgagccgc tcgaagatcg cgagcgactg
     1321 cgtcaagatg ttttggcagc ccggcgcgaa cttgcagtcc ggccccgcca tgacttggcc
     1381 gttctcgtag cggtaggcgt ccggcgcgaa cggtgcatcg acggctgcga aagtcttcgc
     1441 ggcgttcaga cccgtcggat agtgcatggc cgcgtcgatt gcggcctgcc gcgcggcgag
     1501 ctgccctggc cgcggcgcgt agttcatttg ctcgctcggc gcgctcaagc cgtcgatatt
     1561 gaagatcagc ccttccgtgc ggccgcgcgt ggcgacggtc tcgatctccg tgatctgctc
     1621 gtcgacgact ttgaggcgca cgacgagcag caccggcgcg ccgacttcct cgacgacgac
     1681 ggccgcaccg gccacgccgg cgcgctcgtc gagaatgtcc tggcgataac ctcgcagccg
     1741 cgtgacggtg ctcagcagac cgaggttctt gagcggcttc tcgacggcgt cctcggtcac
     1801 gcggacagtg ccggcgaccg gaaccttgct cgcatcgtgc gcgagcagcg cgtgaagaaa
     1861 cgtcgtcatc gtgctgcgca gacactcgcg gtcgcaggcc tccgcggctg acgccgtgcg
     1921 accggccgtc atggcgacgc ctgtcaacag gccgacagca agccaattcg tttgcatcgt
     1981 tgttctcctc aaacgcggtg catgaacttg aacacgtcct cgtgctgcgc gtcgatcttg
     2041 agtcccatct cgaggccttt ggcgtcgagc ttgcgtttca gctcgtcgaa cgacacggtc
     2101 gccttgtcga gatcggccaa catgatcatc gcgaagaact cgcgcacggt cgtttgcgag
     2161 atgtcgatga tgttggcgcg cgcttcggcc aacacggccg agacgttggc aatgatgccg
     2221 accgtgtcgg agccgatgac ggtcacgatg atgcgattcg acttggccgt cattgccgcg
     2281 cgtcgccggg ctggtactcg aaggcgagct ccttgcacgc atcgacggcg aacggcgcgt
     2341 ctgccggcag caccgtatcg ccgctgacgt actcgtcgac gaagtagagc gggtcggtcg
     2401 cgacgtactc gcgtcgcaac gccagcgtcg cgggatcgag tgtgaaccgc tcgacgaggt
     2461 gcagcttgtc gctgtgagcc acggtgccgg ccaggctgcc gggctcgaac gcgacggtgt
     2521 ctacgacgag cgtgtcgccg tcccagcgtc cgatcgagtg accggcgcgg ctcggcgcga
     2581 cgttcgacgg atgcgccgcg aggtccatgt gcaccgtgcg ctcgagccct cggccgtact
     2641 ccatcttgat gacgtcgtcg ccctgcgtga tgcgattgat cgtgccgtcg aagacccagt
     2701 cgaacagaac gctcgtgatc tggcagctca ggcgcgggtt gcgctccggc ggcaggtcgc
     2761 gcagcgcagc ggaggctgcc tggccggcgg ctgtcagcgt cacgggcggc gggaaccagc
     2821 cgtgactcgg cacgtcggcc atcgcgactt tgccggcttc gacggcttcg agcaggctct
     2881 tcggcacgag gtt
//
LOCUS       DATLEE010000261         8945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7599233, whole genome shotgun sequence.
ACCESSION   DATLEE010000261 DATLEE010000000
VERSION     DATLEE010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8945
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7599233"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /gene="rpsT"
                     /locus_tag="VM692_05430"
     CDS             complement(<1..176)
                     /gene="rpsT"
                     /locus_tag="VM692_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HUQ51640.1"
                     /translation="MANIQSAKKRARQAIKRRAHNVTLRSQVRTAIRKVMKAIEAGNK
                     EAAKAEFAVVVPQI"
     gene            274..1872
                     /gene="murJ"
                     /locus_tag="VM692_05435"
     CDS             274..1872
                     /gene="murJ"
                     /locus_tag="VM692_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HUQ51641.1"
                     /translation="MTHEHADRSRGHDRRLLEATSVVGAMTLLSRITGLARDIALSAW
                     FGSGLVMDAFAVAFKLPNLLRRFFAEGAFSQAFVPVISEYRTHKSHAETRELVSHVAG
                     TLAVGLFVVTALGVVAAPLLVLLSAGGLLGEDGRFALAVDMLRLTFPYILFVSLTALA
                     GSVLNSHGRFAAAAFTPVLLNVIMILFAGWIGPLLGEPELGLAAGVLVAGIVQLLFQL
                     PFLRRLGLLPRPRFGLWHAGVRRILKLMAPALFGSSVAQINILLETLIASFLVAGSIS
                     WLYWSDRLIEFPLGVFGIALATVLLPRLSEQHARNAAHTFTATLDWALRLVFVIGVPA
                     TIGLALLSEPVIATLFLRGEFTPHDVTMTAASVRAYAPGLLGFILVKVLAPGYFARQD
                     TRTPVRAGLQALVVGLLFSVAAVLVLLRTDWAPAHAGIAAGTACSALINAALLFTGLR
                     RQGVYRARPGWRALAWRLAVANVVMALVVEAGLSLAGDWLVMSAMTRAGVLAGLVAGG
                     ATVYFVTCHLVGLRASELRIRSVA"
     gene            1882..2811
                     /locus_tag="VM692_05440"
     CDS             1882..2811
                     /locus_tag="VM692_05440"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HUQ51642.1"
                     /translation="MRLYRTFASLPRPADAEPRAVAIGVFDGLHIGHRAILERARGAA
                     DDLRGSSLVLTFEPTPKEFFSPETAPPRLTRFRERFELLEGLGLDELFCAKFGAVREL
                     TARAFIDELLVAKLGARHVVVGDDFRFGADRLGTVEELRTHGRRHGLTVSEVPPVYWN
                     GERVSSTAIRQALKAGDLATARSMLGRDYSISGRVVRGLGLGRKLGFPTANVNLKRLQ
                     APIDGIFAARVAGLGDTPLDGVASVGTRPTIGGGTALLEVLIFDFDRDIYGRYITVQF
                     LKRLREERHFDDLESLKRQMHIDVAAARAALTD"
     gene            2868..6026
                     /gene="ileS"
                     /locus_tag="VM692_05445"
     CDS             2868..6026
                     /gene="ileS"
                     /locus_tag="VM692_05445"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HUQ51643.1"
                     /translation="MFEPVAARYDGPELEKGILELWRRENVFGRIMAEGAARPKFVFY
                     EGPPTANGRPGIHHVLARTFKDLYPRYKTMRGFHCPRKAGWDTHGLPVEHEIEKELGI
                     FDKKRIEQEVGVAKFTAQCRASVMRHVAEWEAMTERMGFWVDFEDAYFTLHNDYIESV
                     WALLKQIWDKGLIYQGYKVVPYDPRIGATLSSHEVAQGYREVEDPSVYVRFRVHGEAG
                     TSFLVWTTTPWTLPSNLLVAVHPDVEYAWVKRADETLILAKPLVATVFKDESVEIVRT
                     AKGRELDGMRYERLFDYLSVDERVAGDICRVRPAEFVTTEDGTGIVHIAPAYGEDDLK
                     LGQRCGLPVLHGVGEDGYFLPAVTPVAGKFFKQADPTIIEILRERGLLFRAQKYLHNY
                     PFGWRTGDPLIYYAKNAWYIRTTEYRQRMVELNQGIKWHPSHIRDGRFGNWLENNIDW
                     ALSRERFWGTPLPLWTDGDGDYICIGSVTELERRAGRKLTDLDLHRPAIDSVTFEHEG
                     REYRRVAEVIDCWFDSGAMPYAQWHYPFENRAVFEKSFPADFICEAIDQTRGWFYTLH
                     AIATMVSDQVAYKNVICLSHIVDADGKKMSKSLGNIINPYDVFNAVGADALRWHFTAR
                     VAPDVQKRVSVDIIADVASSFINTFWNTYAFFVMYARLDGFDRKADVPYAQRPEIDRW
                     ILSLLEQTIATATAALDEYDALRAGAALESFVDQLSNWYVRRNRRRFWKAASGSDKQS
                     AYLTLYECLETVNRLLAPFVPFISEAVHQNLVRAIAPEFPVSVHMTSWPQSDARRLDL
                     ELLAETDVVQRVVGLGRAARNSSKLKVRQPLKRLLIRVPNAAAEAAVRRHEDQILDEL
                     NVKRLEVIARDATLVSYRIKPNLPVIGKRYGKLIPAIRQYLATADGAAIAAAVARGEQ
                     QTFIVGGQSLAIEPADLLVESAAAEGFACAEEGGYLAGLDTTLDDALRREGLARELVR
                     AVQDARKQAGLEVADRIVLSVEGDAAVSAALAEHRDYLMSETLASRWGKPPAAGEFVA
                     AQDQGEAQWVIRLAREPSAEAR"
     gene            6023..6544
                     /gene="lspA"
                     /locus_tag="VM692_05450"
     CDS             6023..6544
                     /gene="lspA"
                     /locus_tag="VM692_05450"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004858664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HUQ51644.1"
                     /translation="MSERRRWFSLQAGASNWLWLSVAVMALDQWTKSIVVERFEEFDR
                     IVLLPILDLMRLHNEGAAFSFLSNAGGWQRWVFIGLGVAVSAGILVWLRRLSGKGQWL
                     LAAGLALILGGALGNVVDRVLLGYVIDFIRVHYEDHYFPAFNVADSAISIGAVLIILD
                     NLLDLDRARVKKA"
     gene            complement(6554..6994)
                     /locus_tag="VM692_05455"
     CDS             complement(6554..6994)
                     /locus_tag="VM692_05455"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HUQ51645.1"
                     /translation="MNKRIAQAPLRRSMLGVTLLELMAVVMVIGILGMIALPSYRQFV
                     MRAQRTEAKNALLRLQTNQERFYLANRTYSADPVALGFTASQSERGTYTIAVGAADAN
                     GYTATATPRAGANIDMTADAQCTSFSITAQGVRTATGTGAANCW"
     gene            complement(7065..>8945)
                     /locus_tag="VM692_05460"
     CDS             complement(7065..>8945)
                     /locus_tag="VM692_05460"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HUQ51646.1"
                     /translation="LLNTGGVGEPTRNQVIDFMNGIDTPDTDQDSSITDPRTQIGDPL
                     HAQPVSVIYGPGLRDGLVFMATNDGYLHAIDLESGIEQWAFVPPEFLANQVDLFKDES
                     TAYKQYGIDGDLRIQMVADNDGIVESGEKVYLFFGVGRGGDFYYGLDVTDPASPQLLW
                     RLDGANLPGLGQAWSAPMPTKVDVAGATQNANHLALVIGGGYEPDQDASTLSTDTIGN
                     SIYIVDSVSGALLWHGSKDGVHKDFNVSGRAMDYSIPSRIRTVDIDGDGKLDRMYAGD
                     MGGQVWRFDVNNGQAAANLVTGGVIAQLGGAPSASPAATAVRRFYNAPDAAFINTRDT
                     NFVHIGIGSGHRGHPLGTSVQDAFYALRDFKLAPMTQAQFDALTIIHHGDLTPVTTAN
                     TVVSNTAPGWRIDLSIGGWNGEKVLSEARTFANQVFFSTFQPSTAAATCEPQLGTNRT
                     YAMSVYNGHPVMNLDDSADPNTLTMSDLFVQAEGGILSSAQALFVDRDSDADGIPDAE
                     DDSDGDGLSDANDADRDGDGVADAQEDADGDGIPNYQDGDDDGDGMNDDVDNDDLRAN
                     AGEDPDGDGIVNSLDNDDDGDGIDDIDDDDDDVVCVALRCFSGVMRNDPIRTAWSQDS
                     VD"
BASE COUNT         1375 a         2911 c         2987 g         1672 t
ORIGIN      
        1 tcgatttgcg ggacgacgac cgcaaactcc gccttcgcgg cttctttgtt gcccgcctcg
       61 atggccttca ttaccttgcg aatggccgtc cggacctggg aacgcaaggt gacgttgtga
      121 gcgcggcgct tgatcgcctg ccgggcacgc ttcttcgctg attggatgtt ggccaaggga
      181 gattccgtag gggccgaagc cctgaaaagg cggctattat tcggaccgtc cacaggcaag
      241 tcaagcacat agctcgcccc gtataactcg ttcatgaccc acgaacacgc ggatcgctcg
      301 cgcggccacg accggcgttt gctcgaagcg acctcggtcg tcggggcgat gacgttgttg
      361 tcgcggatca cggggctcgc acgcgacatc gctctgtcgg cctggttcgg ctccgggctc
      421 gtcatggacg ccttcgcggt ggcgttcaag cttccgaacc tgctgcggcg cttcttcgcc
      481 gaaggcgcgt tttcgcaagc gttcgttccc gtgatctccg agtaccgcac gcacaagagc
      541 catgccgaga cgcgggagct cgtcagtcac gtcgcgggga cgctggccgt cggcctgttc
      601 gtcgtgactg cgctcggcgt cgtcgcggcg ccgctgctgg tgctgctctc ggcgggcggt
      661 ctgctcggcg aggacggccg gttcgcgctt gccgtcgaca tgctgcggct gacgttcccg
      721 tacatcttgt tcgtctcgct gaccgcgctt gccggcagcg tgctgaattc acatggccgc
      781 ttcgcggccg ccgcgttcac gcccgtgttg ctgaacgtga tcatgatcct gttcgcgggc
      841 tggatcgggc cgctgctcgg cgagccggag ctcggtctcg cggccggcgt gctcgtcgcc
      901 ggtatcgtcc agcttttgtt ccaactgccg tttttgcgcc ggctcggttt gttgccgcgg
      961 ccgcgtttcg ggctttggca cgccggcgtc cgccgcatcc tgaagctcat ggcgcccgcg
     1021 ctgttcggct cgtcggtcgc acagatcaac atcttgctcg agacgctgat cgcctcgttc
     1081 ctcgtcgcgg gcagcatcag ctggctctac tggtcggacc ggctcatcga gtttccgctc
     1141 ggcgtgttcg gcatcgcgct ggcgacggtt ttgttgccgc ggttgtcgga gcagcacgcg
     1201 cggaacgccg cgcacacgtt cacggcaacg ctcgattggg cgctgcggct cgtcttcgtc
     1261 atcggtgttc cggcgacgat cgggctcgcg ttgctgtccg agcccgtgat tgcgacgctg
     1321 ttcctgcgcg gcgagttcac gccgcacgac gtgacgatga ccgctgccag cgtgcgtgct
     1381 tacgcgccgg gcctgctcgg cttcatcctc gtgaaagttt tggcgcccgg ctacttcgcg
     1441 cggcaagaca cgcgcacgcc ggtccgcgcc gggctgcaag ctctggtcgt gggtttgctc
     1501 ttcagcgtcg cggccgtcct cgtcttgttg cgcaccgact gggcgcccgc gcacgcgggc
     1561 atcgcggccg gcacggcttg ctcggctctc atcaacgcgg cgctattgtt cacgggccta
     1621 cggcggcagg gcgtctaccg cgcgcggccc ggctggcgcg cgctcgcgtg gcgcctcgcc
     1681 gtcgcaaacg tcgtgatggc gctcgtcgtc gaggccggcc tttcgctggc cggcgattgg
     1741 ctcgtcatga gcgccatgac gcgcgccggc gttttggccg ggctcgttgc cggcggggcc
     1801 accgtatact tcgtcacgtg tcacctcgtc ggcttgcgcg cgagcgagct ccgaattcgg
     1861 tcggtggcct gaagggttca aatgcggctc tatcggacgt tcgcttcgct accgaggccg
     1921 gccgacgccg agccgcgcgc cgtcgcaata ggcgtcttcg acggtttgca catcggccat
     1981 cgagcgattc tcgagcgcgc gcgcggcgcc gctgacgatc tccgcggctc gtcgctggtg
     2041 ctgacgttcg agccgacgcc gaaggagttt ttctcgcccg agacggcacc gccgcgcttg
     2101 acgcgttttc gcgagcgctt cgagctgctc gaggggctcg gcctcgacga gctcttctgc
     2161 gcaaagttcg gcgccgtgcg cgagctgacg gcgcgcgctt tcatcgacga attgctcgtc
     2221 gcgaagctcg gcgcgcggca cgtggtcgtc ggcgacgatt ttcgctttgg cgccgatcga
     2281 ctcggcaccg tcgaggagct gcggacgcac gggcggcggc acggcctcac cgtgtccgag
     2341 gttccgcccg tctactggaa cggcgagcgc gtcagcagca cggcgatccg ccaagcgctc
     2401 aaggccggcg acctcgcgac ggcgcgctcg atgctcggcc gcgactattc gatctcgggc
     2461 cgcgtcgtgc gcgggctcgg tttggggcgc aagctcgggt tcccgacggc caacgtgaat
     2521 ctcaaacggc tgcaggcgcc gatcgacgga attttcgcgg cgcgcgtcgc aggattgggc
     2581 gacacgccgc tcgacggcgt cgcgagcgtc ggcacgcggc cgacgatcgg cggcggcacg
     2641 gcgctgctcg aggtgctgat cttcgatttc gaccgcgaca tctacggccg ctacatcacc
     2701 gtgcagtttc tgaaacgtct gcgcgaggag cggcatttcg acgatctcga gtcgctgaag
     2761 cggcagatgc acattgacgt cgcggctgcg cgggctgcgc taaccgattg aaatcgcgta
     2821 cactcacgcc cctccagctc ggcctcgccg acgcttcaag acagagcatg ttcgaacccg
     2881 tcgccgcgcg ttacgacgga ccggagctcg agaagggaat cctcgagctc tggcgccgtg
     2941 agaatgtctt cggccgcatc atggccgagg gtgccgcgcg ccctaagttc gtgttctacg
     3001 agggcccgcc gacggcgaac ggccggcccg gcattcacca cgtgctggcg cgcacgttca
     3061 aggatctcta tccgcgctac aagacgatgc gcggctttca ctgcccgcgc aaagccggct
     3121 gggacacgca cggcctgccc gtcgagcacg agatcgagaa agagctcggc atcttcgaca
     3181 agaagcgcat cgagcaggaa gtcggcgtcg cgaagttcac ggctcagtgc cgcgcgtccg
     3241 tgatgcgcca cgtcgccgaa tgggaagcca tgacggagcg catgggcttc tgggtcgact
     3301 tcgaggacgc gtatttcacg ctgcacaacg actacatcga aagcgtctgg gctttgctga
     3361 agcagatctg ggacaaaggt ctgatttatc aaggttacaa ggtcgtgccg tacgacccgc
     3421 gcatcggcgc gacgttgtcg tcgcacgagg tcgcgcaagg ctatcgcgag gtcgaagacc
     3481 cgtcggtcta cgtgcgattc cgcgtgcacg gcgaggccgg cacgtcgttc ctcgtctgga
     3541 cgacgacgcc gtggacgctg ccatcgaacc tgctggtggc cgtgcacccc gacgtcgagt
     3601 acgcctgggt caagcgcgct gacgagacgc tgattctcgc gaagccgttg gtcgcgacgg
     3661 tgtttaagga cgagagcgtc gagatcgtcc gcaccgcgaa gggacgcgag ctcgacggca
     3721 tgcgttacga gcggctgttc gattatctct ccgtcgacga gcgcgtcgca ggcgacatct
     3781 gccgcgtgcg gccggccgag ttcgtgacga ccgaggacgg cacgggcatc gtgcacatcg
     3841 cgccggcgta cggcgaggac gacttgaagc tcggacaacg ctgcggcttg cccgtgctgc
     3901 acggcgtcgg cgaggacggc tatttcttac cggctgtgac acccgtcgcg ggcaagttct
     3961 tcaagcaggc cgacccgacg atcatcgaga tcctgcgtga gcgcggcctc ctgtttcgcg
     4021 cgcagaaata cctgcacaac tatccgttcg gctggcgcac cggcgacccg ctgatctact
     4081 acgcgaagaa cgcgtggtac atccgcacga ccgagtaccg gcagcgcatg gtcgagctca
     4141 atcagggcat caagtggcat ccttcgcaca tccgcgacgg ccggttcggc aactggctcg
     4201 agaacaacat cgactgggcg ttgtcgcgcg agcgcttctg gggcacgccg ttgccgctgt
     4261 ggaccgacgg cgacggcgac tacatctgca tcggttccgt taccgagctc gagcggcgcg
     4321 cgggccgcaa gctgacggac ctcgacttgc accggcccgc gatcgacagc gtgacgttcg
     4381 agcacgaggg ccgcgaatac cgccgcgtcg ccgaggtcat cgactgctgg ttcgactcgg
     4441 gcgcgatgcc gtacgcgcaa tggcattacc cgttcgagaa ccgcgccgtc ttcgaaaaga
     4501 gcttcccggc cgacttcatc tgcgaagcga tcgatcagac gcgcggctgg ttctatacgc
     4561 tgcacgcgat tgcgacgatg gtgtccgacc aagtcgccta caagaacgtg atctgcctga
     4621 gccacatcgt cgacgccgac ggcaagaaga tgtcgaagtc gctcggcaac atcatcaatc
     4681 cgtacgacgt gttcaacgcc gtcggcgcgg acgcgctgcg ttggcacttc acggcgcgcg
     4741 tcgcgcccga cgtgcagaag cgcgtctccg tcgacatcat cgccgacgtc gcgagcagct
     4801 tcatcaacac gttctggaat acctacgcgt tcttcgtgat gtacgcgcgg ctcgacggct
     4861 tcgaccgcaa ggccgacgtc ccgtatgcgc agcgtcccga gatcgatcgc tggatcctgt
     4921 cgctgctcga gcagacgatc gcgacggcca cggcggcgct cgacgagtac gatgcgctgc
     4981 gcgccggcgc ggcgctcgag agcttcgtcg atcagctcag caattggtac gtgcggcgca
     5041 atcggcgccg cttctggaaa gccgcgagcg gcagcgacaa gcagtccgcg tatctgacgc
     5101 tgtacgagtg cctcgagacc gtgaaccgct tgctggcgcc gttcgtgccg ttcatcagcg
     5161 aagccgtgca ccagaatctc gtgcgtgcga tcgcgccgga atttcccgtc agcgtgcaca
     5221 tgacgtcctg gccgcagagc gacgctcggc ggctcgacct cgagctgctg gccgagacgg
     5281 acgtcgtgca gcgcgtcgtc ggcctcggcc gcgccgcgcg caactcgtcg aagctcaagg
     5341 tgcgccagcc gctgaagcgc ctgctgatcc gcgtgccgaa cgcggctgcc gaagctgcag
     5401 tgcgccgcca cgaggatcaa attctcgacg agctcaacgt caaacgcctc gaggtcatcg
     5461 cgcgcgacgc gacgctcgtg agctaccgca tcaagccgaa cttgcccgtc atcggcaaac
     5521 gctacggcaa gctgatcccg gcgatccgcc agtacctcgc aacggccgat ggcgcagcga
     5581 ttgcggccgc cgtcgcgcgc ggcgagcagc agacgttcat cgtcggcggc cagtcgctcg
     5641 caatcgagcc ggccgatctg ctcgtcgaaa gcgcggccgc cgaaggtttc gcgtgcgccg
     5701 aggagggcgg ctacctcgcc ggcctcgaca cgacgctcga cgacgcgttg cgccgcgaag
     5761 gcctcgcgcg cgagctcgtg cgcgcggtgc aggacgctcg caagcaagcc ggactcgagg
     5821 tcgcagaccg tatcgtgctg tccgtcgaag gcgatgccgc cgttagcgcg gcgctcgcgg
     5881 agcatcgcga ctacttgatg agcgagacgc tcgcgagccg ctggggtaaa ccacccgctg
     5941 ccggcgagtt cgtcgccgcg caggatcaag gcgaagcgca gtgggtcatc cggctcgcgc
     6001 gcgagccgag tgcggaggcg cgatgagcga gcggcggcgc tggttctcgt tgcaggccgg
     6061 cgcgtcgaat tggctgtggc tcagcgtcgc cgtgatggcg ctcgatcagt ggacgaagtc
     6121 catcgtcgtc gagcggttcg aggagttcga tcgaatcgtc ctgctgccca tcctcgacct
     6181 catgcgtttg cacaacgaag gcgccgcgtt cagcttcttg agcaacgccg gcggctggca
     6241 gcgctgggtg ttcatcggtc tcggcgtggc cgtcagcgcg ggaattctcg tctggctgcg
     6301 gcggttgtcg ggcaagggtc agtggttatt ggccgccggc ttggcgctga tcttgggcgg
     6361 cgcgctcggc aacgtcgtcg atcgcgtgct gctcggctac gtgatcgatt tcatccgcgt
     6421 gcattacgaa gaccactact tcccggcctt caacgtcgcg gactccgcga tctcgatcgg
     6481 cgccgtgctg attatcctcg acaacttgct cgatctcgat agagcgcgcg tcaaaaaggc
     6541 gtagcgagcc cggttaccag cagtttgccg cgccggtgcc cgtggccgtg cggacgcctt
     6601 gagcggtgat cgagaacgac gtgcactggg catccgccgt catgtcgatg ttggcgccgg
     6661 cccgtggcgt cgctgtcgcc gtgtagccgt tcgcgtcggc ggcgccgacg gcaatcgtgt
     6721 aggtgccgcg ttcgctttgg ctggccgtga agccgagcgc gacgggatct gcggaatagg
     6781 tgcggttcgc aaggtagaac cgttcttggt tcgtctgcag tcgcagcaat gcgttcttcg
     6841 cttccgtgcg ctgcgctcgc atgacgaact gccgatagct cgggagggca atcatgccga
     6901 ggatgccgat gaccatgacg acggccatga gctcgagcag cgtcacgccg agcatgctac
     6961 gccgcagcgg cgcctgagcg attcgcttgt tcattgcagt ctctcttccc taagtttcgt
     7021 agcgccctgc gggccgggtc gcgaacgagc ccggcccgcg ggaatcagtc aacgctatcc
     7081 tgcgaccaag ctgtgcggat cggatcgtta cgcatgacgc ccgagaagca gcgcagcgcc
     7141 acgcagacga catcgtcatc gtcgtcgtcg atgtcgtcaa ttccgtcgcc gtcgtcgtcg
     7201 ttgtccagcg agttgacgat gccgtcgccg tccgggtctt cgccggcgtt ggcgcgcaag
     7261 tcgtcgttgt ccacgtcgtc gttcatgccg tcgccgtcgt cgtcgccgtc ctggtagttc
     7321 ggaataccgt cgccgtcggc gtcctcctgc gcgtcggcca cgccgtcgcc atccctgtcg
     7381 gcgtcattcg cgtcggacaa gccgtcgccg tcgctgtcgt cctcggcatc ggggatgccg
     7441 tcggcgtcgc tatcgcgatc gacgaacagc gcttgcgccg acgacagaat accgccctcg
     7501 gcctgcacga acaggtcgct catcgtgagg gtgttggggt ccgccgaatc gtcgaggttc
     7561 atgacggggt ggccgttata gaccgacatc gcgtaagtac ggttcgtgcc gagctgtggc
     7621 tcgcacgttg cggcggccgt gctcggttgg aatgtcgaga agaacacctg gtttgcaaac
     7681 gtgcgtgcct cggacagcac cttctcgccg ttccagccgc cgatgctgag atcgattcgc
     7741 cagccgggag ccgtgttgga gacgacggtg ttggccgtcg tcacgggcgt gaggtcgcca
     7801 tgatgaatga tcgtgagcgc gtcgaattgg gcctgcgtca tcggcgcgag cttgaagtcg
     7861 cgcagcgcgt agaacgcgtc ctgaacggac gtgcccagcg gatggccgcg gtggccggag
     7921 ccgatgccga tgtgcacgaa gttggtatcg cgcgtgttga tgaacgccgc gtccggcgcg
     7981 ttgtagaacc gacgaaccgc cgtggccgcg gggctcgccg agggtgcacc gccgagttga
     8041 gcgatgacac cgcccgtgac caggttcgcg gccgcttggc cgttgttgac gtcgaagcgc
     8101 caaacttgcc cgcccatgtc gccggcatac atgcgatcaa gcttgccgtc gccgtcgata
     8161 tcgacggtgc gaatgcgcga cggaatcgaa tagtccatcg cacgaccgct cacgttgaag
     8221 tccttgtgca cgccgtcttt gctgccatgc cagagcagcg cgcccgacac ggagtcgacg
     8281 atatagatcg agttgccgat cgtgtccgtc gacagcgttg aagcgtcctg gtcgggctcg
     8341 tagccgccgc cgatgacgag tgcgaggtgg ttggcgttct gcgtggcgcc tgccacgtcg
     8401 accttcgtcg gcatcggcgc cgaccaagct tggccaagcc ccggcaagtt cgcgccgtcg
     8461 aggcgccaca gcagctgcgg cgacgcgggg tccgtcacgt cgaggccgta atagaagtcg
     8521 ccgccgcgac cgacgccgaa gaacaagtag accttctcgc cggactctac gatgccgtcg
     8581 ttgtcggcga ccatctggat gcgtagatct ccgtcgatgc cgtactgctt gtaagccgtc
     8641 gactcgtcct tgaagagatc gacctggttg gccaggaact ccggcggcac gaacgcccac
     8701 tgctcgatgc cgctctcgag gtcgatggcg tgtaggtagc cgtcattcgt ggccatgaag
     8761 accaagccgt cgcgtaggcc agggccataa atcacggaga ccggctgtgc atgcagcggg
     8821 tcgccgatct gggtgcgcgg atcggtaatg ctgctgtcct ggtcggtatc cggcgtgtcg
     8881 ataccgttca tgaagtcgat gacctgattg cgcgtgggct cgccgacgcc gcccgtattg
     8941 agcag
//
LOCUS       DATLEE010000262         3152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1044159, whole genome shotgun sequence.
ACCESSION   DATLEE010000262 DATLEE010000000
VERSION     DATLEE010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044159"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VM692_05465"
     CDS             <1..150
                     /locus_tag="VM692_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HUQ51647.1"
                     /translation="RNIAVALGNAPPDPRVVAALEARRDEASAIVREHVAWALARQRA
                     AVSRP"
     gene            227..1219
                     /locus_tag="VM692_05470"
     CDS             227..1219
                     /locus_tag="VM692_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HUQ51648.1"
                     /translation="MSMVNLESLAHAVDTDFRRSLLQGIALFRGIDAESLSDLLPQCG
                     RIDVRPGHVLLSPERENHCVYVVLSGRLSVHLGSLDAPKIADLGPGSCAGEMSLIENK
                     DPSAFVVATEDCHLMVISHTLLWRMVDRSHTFCKNLLIVLSERVRSDNEYIASSLGVL
                     RQAERNAHTDALTGLGNRHWMQTMFEREVMRTLHSKKALCLMMVDVDNFKVFNDEYGH
                     IAGDRVLVAVAEALREFLRPTDLVARFGGDEFAVLLPELKVKQARQTAERIRQQVAAL
                     SPTSLSSAITVSIGIADRTDDDDVATLVQRADGALYDAKEAGRNRVAVASALDE"
     gene            complement(1208..2071)
                     /gene="panC"
                     /locus_tag="VM692_05475"
     CDS             complement(1208..2071)
                     /gene="panC"
                     /locus_tag="VM692_05475"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HUQ51649.1"
                     /translation="MATNAVRVVHTVSDVRAAVAAWRQAGETVAFAPTMGNLHAGHMS
                     LAALGAQAARRVVMSIFVNPTQFGPSEDFAAYPRTLAADEALVAPAGNVDLLFVPSAA
                     EIYPFGIEQAVRVQLPALSRELDGASRPGHFDGVATVVCRLLNIVTPDVLFLGQKDYQ
                     QFVLMQRMIADLALPVQLRMGPTHREPDGLAMSSRNRYLDERQRQQAPALHRALARVR
                     EQVLEGARDFERATAAARAELERAGFAPDYVEVRRQGDLAAANPAADEQRIVLGAARL
                     GRARLIDNLFV"
     gene            complement(2082..2915)
                     /gene="panB"
                     /locus_tag="VM692_05480"
     CDS             complement(2082..2915)
                     /gene="panB"
                     /locus_tag="VM692_05480"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HUQ51650.1"
                     /translation="MYKQLEQRGMDSPPVNVGTLREMKQIREPIACLTAYDASFAQLV
                     DLAGTDVVLVGDSLGMVIQGLDTTVPVTVDDIVYHSRAVQRGLRRAFLMVDMPFMSYT
                     TREQALDNAIRIMQEGGAKMVKLEIGRGQVGIIEYLASQDIPLCAHLGLKPQSVHKLG
                     GFKVQGREQQAAQRMLEDARSLESAGADTWLLECGPNALGAAIRDAVQIPVIGIGAGP
                     GVDGQILVLYDVLGITQGRMPRFVKNYLTGRDSPLEALRAYVRAVKDRSYPAAEHTFA
                     E"
     gene            complement(2937..>3152)
                     /locus_tag="VM692_05485"
     CDS             complement(2937..>3152)
                     /locus_tag="VM692_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HUQ51651.1"
                     /translation="YEQLIEAEYLERLGDAYARFFYDFDAAPLLIVNAASIDPIHRET
                     DYQELLQTILKVKHGRHFFNPAGAPLT"
BASE COUNT          529 a         1068 c         1016 g          539 t
ORIGIN      
        1 cgcaacatcg cggtcgctct cggcaacgct ccgccggacc cgcgcgtcgt cgccgcgctc
       61 gaggcacggc gcgacgaagc gtccgccatc gtgcgcgagc atgtcgcatg ggcactcgcg
      121 aggcagcgcg cggccgtgag ccgcccctaa gactgccaac tccgtcaccg tcgcgcttgt
      181 aagcgcgcct tagcatcggc tcgaactctg gcccgcggac ttcccaatga gcatggtcaa
      241 tctcgagtcg ctggctcacg ccgtcgatac ggacttccga cgctctctgc tgcagggcat
      301 cgcgttgttc cgcggcatcg acgccgagag cttgagcgac ctgctgccgc agtgcggccg
      361 catcgacgtc cgtccgggtc acgtcctact ctcgcccgag cgcgagaatc attgcgtcta
      421 cgtcgtgctc agcggccgcc tctccgtgca cttggggtcg ctcgacgcgc cgaagatcgc
      481 cgatctcggc cccggctcgt gcgccggcga gatgtcgctg atcgaaaaca aagatccgtc
      541 ggcgttcgtc gtcgcgacgg aagactgcca tctcatggtc atcagccata cgctgctgtg
      601 gcgcatggtc gatcgctcgc atacgttctg caaaaacctg ctcatcgtct tgtcagagcg
      661 cgtgcgctcc gacaacgagt acatcgcgag cagcctgggc gtgctacgcc aagccgagcg
      721 caatgcgcac acggacgcgc tgacggggct cggcaatcgt cattggatgc agacgatgtt
      781 cgagcgcgag gtcatgcgca cgctgcacag caagaaagcg ttgtgcctga tgatggtcga
      841 cgtcgacaac ttcaaagtgt tcaacgacga gtacgggcac atcgccggcg accgcgtgct
      901 cgtggccgtc gcggaagcgc tgcgcgaatt cttgaggccg acggatctcg ttgctcgctt
      961 cggcggcgac gagttcgccg tgctgctgcc ggagctcaag gtcaagcagg cgcgccaaac
     1021 ggccgagcgc attcgccagc aagtcgccgc gctgtcgccg acgagcctgt cgagcgcgat
     1081 cacggtttcg atcggcatcg cggaccgcac cgacgatgac gatgtcgcca cgctcgtgca
     1141 gcgcgccgat ggcgccttgt acgatgccaa ggaagcggga cgtaaccgag tcgcagtggc
     1201 gtcggcctta gacgaataga ttgtcgatga gccgcgcacg cccgaggcgc gcggcaccga
     1261 gcacgatccg ttgctcgtcc gccgcggggt tcgccgccgc gaggtcgccc tgacgccgca
     1321 cctcgacgta gtccggggca aagccagcgc gctcgagctc ggcgcgcgcg gcagccgtcg
     1381 cgcgttcaaa atcccgcgca ccctcgagca cctgctcgcg cactcgcgcc aacgcgcggt
     1441 gcaatgccgg cgcctgctgg cgctgccgct catcgagata acgattgcgc gagctcatcg
     1501 ccaggccgtc gggctcgcga tgcgtggggc ccatgcgtag ctgcacgggc aacgccaagt
     1561 ccgcgatcat gcgctgcata agcacgaact gctgatagtc cttttgtccc agaaacagca
     1621 cgtcgggcgt gacgatgttc aacaggcggc acacgaccgt cgcgacgccg tcgaagtgcc
     1681 ccggcctgct ggcgccgtcg agctcgcggc tcagcgccgg aagttgcacg cgcacggcct
     1741 gctcgatccc gaacggataa atttcggcgg cgctcggcac aaacagtaga tcgacgttcc
     1801 cggccggcgc gaccagcgct tcgtcggccg cgagtgttcg cgggtatgcc gcgaagtcct
     1861 cgctcgggcc gaattgcgtc ggattgacaa agatgctcat gacgacgcgg cgcgcggcct
     1921 gtgccccgag cgctgcgaga ctcatgtgcc ccgcgtgcag attgcccatc gtcggcgcga
     1981 acgcaactgt ttcacctgct tggcgccacg ccgcgacggc ggcgcggacg tcgctgaccg
     2041 tgtgcaccac gcgaacggca ttcgttgcca ccggcttcac ttcattcggc gaacgtgtgt
     2101 tccgcggccg gataactgcg gtccttgacc gcgcggacgt acgcacgcaa cgcctcgagc
     2161 ggcgagtcgc gtcccgtcag atagttcttc acgaagcgcg gcatgcgccc ctgcgtgatg
     2221 ccgagcacgt cgtagagcac gaggatctgg ccgtcgacgc cgggcccggc gccaatgccg
     2281 atcacgggaa tctgcacggc gtcgcgaatc gcagcgccaa gcgcgttcgg cccgcactcg
     2341 agcaaccacg tgtcggcgcc cgcgctctcg agcgagcgcg cgtcctcgag cattcgctgc
     2401 gccgcctgct gctcccgccc ttgaaccttg aaaccgccga gcttgtgcac cgattgcggc
     2461 ttgaggccca ggtgcgcaca gagcggaatg tcctggctcg caaggtactc gatgattccg
     2521 acctggccgc ggccgatctc gagcttgacc atcttcgcgc cgccttcctg catgatgcga
     2581 atcgcgttgt cgagcgcctg ctcgcgcgtc gtgtagctca tgaacggcat gtcgaccatg
     2641 aggaaggcgc ggcgcaaacc gcgctgcacg gcgcggctgt gatagacgat atcgtcgacg
     2701 gtgacgggca ccgtagtatc aagaccctgg atgaccatgc cgaggctgtc gccgacgagc
     2761 acgacgtcgg tgccggcgag gtcgacgagc tgcgcgaagc tcgcgtcgta cgccgtcaga
     2821 caggcgatcg gctcacggat ctgcttcatt tcccgcagcg ttccgacgtt gacgggcggc
     2881 gaatccatgc cgcgctgctc gagttgcttg tacatttgga cctccgcccg ctcggctcag
     2941 gtcagcggcg ccccggccgg gttgaagaag tgccggccgt gcttgacctt gagaatcgtc
     3001 tggagcagtt cctgatagtc agtttcgcga tgaatcgggt caatgctcgc tgcgttgacg
     3061 atcagcaacg gcgcggcatc gaagtcgtag aagaaccgcg cgtacgcatc gcccagccgc
     3121 tcgagatact cggcctcgat gagctgctcg ta
//
LOCUS       DATLEE010000263         2251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8345700, whole genome shotgun sequence.
ACCESSION   DATLEE010000263 DATLEE010000000
VERSION     DATLEE010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8345700"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1680
                     /gene="guaA"
                     /locus_tag="VM692_05490"
     CDS             94..1680
                     /gene="guaA"
                     /locus_tag="VM692_05490"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HUQ51652.1"
                     /translation="MTKTATARAAQKEPVSHAERILVVDFGGQYTQLIARRVREVGVY
                     SEILPWDAPAEQIAAFAPKGIILSGGPESVAEAGSPQINDALLAAGAPVLGICYGMQA
                     LAAKLGGAVASADHREYGYAEVDLQGNDPLLSKLGARLKVWMSHGDRVERLPQGFAAI
                     GSSSNSPLAAMADIERKIYALQFHPEVTHTEHGLTIIRRFVLDICGCAPVWTAKNIVA
                     EHIGQIRAKVGNDRVLLGLSGGVDSSVVAALLHEAIGDQLVCVFVDTGLLRLNEGDQV
                     MAIFAKHLGANVIRVDAEKKFLSALAGVDDPEQKRKIIGRLFVEVFDAEAEKIKDVRW
                     LAQGTIYPDVIESAGAKTGKAHLIKSHHNVGGLPERMNLKLLEPLRDLFKDEVRKIGT
                     ELGLPKELVGRHPFPGPGLGVRILGEVKKEYADLLRRADDIFISELRAQGLYDKVSQA
                     FAVFLPVRSVGVMGDGRRYDYVVALRAVETVDFMTAHWSRLPYEFLDVVSRRIINEVP
                     GISRVTYDISGKPPATIEWE"
     gene            1905..>2251
                     /locus_tag="VM692_05495"
     CDS             1905..>2251
                     /locus_tag="VM692_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HUQ51653.1"
                     /translation="MSPATPQEQILGIVNNHWQSCCVGAVAQLELADQLAEGPLPIDI
                     LAERTKTHGPSLYRMLRALESTGIFTQTSPGVFGNTPASDCLRRNLAGSNWAWIRFTL
                     CSGAPVFEGWRGL"
BASE COUNT          423 a          713 c          718 g          397 t
ORIGIN      
        1 gaagttcgtg cggatcacgg gcgccggccg ccgcgagagc cacgtgcacg acgtcacgat
       61 caccaaagaa ccgccgaact accgctccga ggtatgacga agaccgctac cgcgcgtgca
      121 gcgcaaaagg agccggtaag ccacgccgag cgcattctcg tcgtcgactt cggcggccag
      181 tacacgcagc tgatcgcgcg gcgggtccgc gaggtcggcg tctacagcga gatcctgccg
      241 tgggacgcgc ccgcggagca aatcgccgcg ttcgcgccga agggcatcat tctctcaggc
      301 ggccccgagt cggtcgccga agcgggctcg ccgcaaatca acgacgcgtt gctcgccgcc
      361 ggcgcgcccg tgctcggcat ctgctacggc atgcaagcgc tcgcggccaa gctcggcggc
      421 gccgtcgcgt cggccgatca ccgcgagtac ggctatgccg aagtcgactt gcagggcaac
      481 gatccgctgt tgtcgaagct cggcgcgcgc ttgaaggttt ggatgagcca cggcgaccgc
      541 gtcgagcgct taccgcaggg cttcgcggcc atcggctcgt cgtcgaactc gccgctcgcg
      601 gcgatggccg acatcgaacg caagatctac gcgctgcagt tccatcccga agtcacgcac
      661 acggagcacg gcctcacgat cattcgccgc ttcgtgctcg acatctgcgg ctgcgcgccg
      721 gtctggacgg cgaagaacat cgtcgccgag cacatcgggc agattcgcgc gaaggtcggc
      781 aacgaccgcg tgctgctcgg tttgtccggc ggcgtcgact cgtcggtcgt agcggcgcta
      841 ttgcacgagg caatcggcga tcagctcgtc tgcgtgttcg tcgacacggg cctgctgcgc
      901 ttgaacgaag gcgaccaggt catggcgatc ttcgccaagc acttgggcgc gaacgtgatc
      961 cgcgtggacg ccgagaagaa gtttctctct gcgctcgcag gcgtcgacga tcccgagcaa
     1021 aagcgcaaga tcatcgggcg gctgttcgtc gaagtgttcg acgccgaggc cgagaagatc
     1081 aaggacgtac gctggttggc acaaggcacg atctatcccg acgtcatcga gtcagccggc
     1141 gccaagaccg gcaaagcgca cctgatcaaa tcgcatcaca acgtcggcgg cttgcccgag
     1201 cgcatgaact tgaagctgct cgagccgctg cgcgatttgt tcaaagacga agtgcgcaag
     1261 atcggcaccg agctcgggct gccgaaggag ctcgtcgggc gtcacccttt cccggggccg
     1321 gggctcggcg tgcgcatcct cggcgaagtc aagaaggagt acgcggactt gctgcgccgc
     1381 gccgacgaca tcttcatctc ggagctacgt gcgcaaggtc tctacgacaa agtgagccag
     1441 gcgttcgcgg tatttttgcc cgtgcgttcc gtcggcgtca tgggcgacgg ccgccgctac
     1501 gactacgtcg tcgcgctgcg cgccgtcgag accgtcgatt tcatgacggc gcactggtcg
     1561 cgcttgccgt acgaattctt ggacgtcgtg tcgcgccgca tcatcaacga ggtgcccggc
     1621 atctcgcgcg tgacgtacga catctcgggg aagccgcctg caacgatcga gtgggagtga
     1681 ctgcaaatct ctacgctacc aagacgctgt cggttaaagc agccgttgtt tccgggaaaa
     1741 actggcgcaa accgcaacgt cggtgtgtcg gtagtgaacg actcgagtag tgatcattag
     1801 agtcgttggg tagcatcacg ttaacgagct gtcgacggtc gacaatcgcc caacccggcc
     1861 cctcgacccg gtattctcca aacccaacaa gaaagggaat cgcgatgagc cctgctacgc
     1921 cccaagagca aattctcggt atcgtcaaca accattggca gagttgctgc gtcggtgctg
     1981 tcgcccaact cgaactcgcc gatcagctgg ccgagggtcc cttgcccatc gacattcttg
     2041 ccgagcgcac aaagacgcac ggaccgagcc tgtatcggat gttgcgggct ctggagagca
     2101 cggggatatt cacgcagacc tcgcccggag tgttcggcaa cacgccggcg agtgattgcc
     2161 tccgccgtaa tctcgcgggc tctaattggg cttggattcg ctttacgctc tgctcgggcg
     2221 ctccggtatt cgaggggtgg agagggttga t
//
LOCUS       DATLEE010000264         2999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2536726, whole genome shotgun sequence.
ACCESSION   DATLEE010000264 DATLEE010000000
VERSION     DATLEE010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2536726"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VM692_05500"
     CDS             <1..508
                     /locus_tag="VM692_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUQ51654.1"
                     /translation="RLDDAQAESLEPGHAARSALLARTLTAAPSAGQVKLVKRLAWRA
                     APRDEAPEPQLPPSAPLAARAAVPTHLVYGGYAYRVGSEGVLIGRETDSQRRTIVVGG
                     ANSGVSRAHCEVVLRDGELKLRDLSSFGTFVNERKVLSEIALERADVIRVGSPGAELH
                     VVSLEGAE"
     gene            505..1599
                     /locus_tag="VM692_05505"
     CDS             505..1599
                     /locus_tag="VM692_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUQ51655.1"
                     /translation="MRKRTRELNVFSMSFLDAITAGFGCVVLLFMLVSQNALLDNRSS
                     VDNLEAEAKRWELRVLTGQRNLVQIQERLEAQLRDWTALRVMRENLVTEIDTTQAELA
                     AVTQDSAARQAAIERLRAELAALQKESEQVPVAGRPDDEGTRLRSFEGEGNRQYLTGL
                     RMGGKNVVILVDTSTSMLDRTIVNILRRRNMAPAQQRRSPKWQQLVNTVDWLTTQIAP
                     GTQVQVIGFNDKATWLIPGSEGKWVTVESGSELDAPVKALRDSYPQGPTSLAVAFNAI
                     KMLDPKPDNIYLLTDGLPTMGDVIPTREGVNAKDRLDHWDRAVRQLPVGVPVNTILFA
                     MEGDPQAAPAYWMLAIRTGGSMMAPSEDWP"
     gene            1596..2612
                     /locus_tag="VM692_05510"
     CDS             1596..2612
                     /locus_tag="VM692_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUQ51656.1"
                     /translation="MKRRRDFDIFSMSFLDAVCCGFGAMILLFIVTRTSEPARLEESE
                     RDLNGLIAQYEQELNDIVGETERVQRNETSTTASVQTDRRRIEDLREQLEQIRAQVLA
                     TAEDTELTQEVQNRLASAKQSLTDEMKRLLADYRPPIDDYKVGGIPVDSEYIIFLIDT
                     SGSMRGYQWERVQQQLRETLEVYPTVKGIQVMNDEGEYMFKSYRDEWIPDTPTRREAI
                     LDALKSWDAFSNSNPREGILAAIDKFYDPNKKISLYVYSDDFQQGSINPVVREVDRRN
                     RANATGERLVRIHAVAFPAVYLMVGELYTAANYATLMRIICQRNGGTFVALPVVRDGR
                     RNRD"
     gene            2666..>2999
                     /locus_tag="VM692_05515"
     CDS             2666..>2999
                     /locus_tag="VM692_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51657.1"
                     /translation="MVVKQNYLGDRVGKLGILVVITVIVQIIYAGYVRPTAEAWRAEQ
                     RAIAARTPGYTPERSIYVIIQDPEQQATIIVCIWAMILSVMRFHEIRAQRELLTAGYF
                     TKDANTVIL"
BASE COUNT          558 a          979 c          983 g          479 t
ORIGIN      
        1 gaggctcgac gacgcgcaag ccgagtcgct cgagcccggg cacgcggcgc gtagcgcgct
       61 gctcgcgcgc acgctgacgg cggcgccgtc cgcggggcag gtcaagctcg tcaagcgtct
      121 cgcctggcga gcagcgccgc gagacgaggc gccggaaccg caactgccgc cgtcggcgcc
      181 gctcgctgcg cgagccgccg tgccgacgca tctcgtctac ggcggctatg cgtatcgcgt
      241 ggggtccgaa ggcgtgctga tcggccgcga gacggactcg cagcgccgca cgatcgtcgt
      301 cggcggggcg aacagcggcg tctcgcgtgc gcactgcgag gtcgtgttgc gcgacggcga
      361 gctgaagctg cgcgatttga gcagcttcgg cacgttcgtc aacgagcgca aagtcctcag
      421 cgagattgct ctcgagcgcg ccgacgtcat tcgcgtcggc tcgccggggg ccgagctgca
      481 cgtcgtcagc ttggagggcg ccgagtgaga aagcgcacgc gcgagctcaa cgtgttttcg
      541 atgtcgttcc tcgacgcgat cacggcgggc ttcggctgcg tcgtgctgct gttcatgctc
      601 gtgagccaga acgcgttgct cgacaaccgc tcttccgtcg acaacctcga ggccgaggcc
      661 aaacgctggg agctgcgcgt tctgacgggg cagcgcaatc tcgtgcagat ccaggaacgg
      721 ctcgaagcgc agctgcgcga ttggaccgcg ctgcgcgtca tgcgcgagaa cctcgtcacc
      781 gaaatcgata ctacgcaggc cgagctcgcg gcagtgacgc aggactcggc cgcgcggcaa
      841 gcggcgatcg agcggctgcg cgccgagctc gccgccttgc aaaaggaatc cgagcaagta
      901 cctgtcgccg gtcggcccga cgacgagggc acgcgcctgc gctcgttcga gggcgaaggt
      961 aaccggcagt acctgacggg actgcggatg ggcggcaaga acgtcgtgat actcgtcgat
     1021 acgtcgacga gcatgctcga ccgaacgatc gtcaatatcc tgcggcgccg caacatggcc
     1081 cccgcgcagc agcgccggtc gccgaagtgg cagcaactcg tcaacaccgt ggactggctc
     1141 acgacgcaga tcgcgcccgg cacgcaggtg caggtcatcg gcttcaacga caaggcgacg
     1201 tggctgatcc cgggttcgga aggcaagtgg gtgacggtcg agtccggcag cgagctcgat
     1261 gctcccgtaa aagcactgcg cgacagctac ccgcaaggtc cgacgagcct ggccgtggcc
     1321 ttcaacgcga tcaagatgct cgacccgaag cccgacaaca tctacttgtt gaccgacggc
     1381 ttgccgacga tgggcgacgt cataccgacg cgcgaaggcg tcaacgccaa ggaccgtctc
     1441 gaccactggg atcgggccgt gcggcagctg cccgtcggcg tccccgtcaa cacgatcctg
     1501 tttgcgatgg agggcgaccc gcaagccgcg ccggcctatt ggatgctcgc gatccgcacg
     1561 ggcggctcga tgatggcgcc gtcggaggac tggccatgaa gcgccgccgc gatttcgaca
     1621 tcttcagcat gtcgttcctc gacgcggtgt gctgcggctt cggcgccatg atcctgctgt
     1681 tcatcgtcac gcgcaccagc gagcctgcgc ggctcgagga gagcgagcgc gatctgaacg
     1741 gtctcatcgc gcagtacgag caagagctca acgacatcgt cggcgagacc gagcgcgtgc
     1801 agcgcaacga aacgagcacg actgccagcg tgcaaacgga ccgccgccgc atcgaggatt
     1861 tgcgcgagca gctcgagcag attcgcgcgc aggtgctcgc gacggccgag gacaccgagc
     1921 tcacgcagga ggtgcagaac cgcctcgcgt ccgcaaagca aagcctcacg gacgaaatga
     1981 aacgattgct ggccgattac cgcccgccta tcgacgacta caaagtcggc ggcattcccg
     2041 tcgacagcga gtacatcatt ttcctgatcg acacctcggg cagcatgcgg ggctaccaat
     2101 gggagcgcgt gcagcagcag ttgcgcgaga cgctcgaggt ctacccgacg gtcaagggta
     2161 ttcaggtcat gaacgacgag ggcgagtaca tgttcaagag ctatcgggac gagtggatcc
     2221 ccgatacgcc gactcgccgc gaagcgattc tcgacgcgct gaagagctgg gacgcgtttt
     2281 cgaactcgaa tccgcgcgag ggcatcctcg ccgcgatcga caagttctac gatccgaaca
     2341 agaagatttc gctgtacgtc tacagcgacg acttccagca gggctcgatc aaccccgtcg
     2401 tgcgcgaggt cgatcgccgt aaccgtgcga acgcgaccgg cgagcgcctc gtgcgcatcc
     2461 acgccgtcgc gttcccggcc gtctacttga tggtcggcga gctctatacc gccgccaatt
     2521 acgcgacgct gatgcgcatc atctgtcagc gcaacggcgg aactttcgtg gcgctgcccg
     2581 tcgtgcgcga cgggcgtcgc aacagggatt gacgttccac ttgttcggca gcgccgcgcg
     2641 ctgcgctttt ggtttggagg ctcgaatggt cgttaagcaa aattatctcg gtgaccgcgt
     2701 cggcaagctc ggcatccttg tcgtcatcac cgtcatcgtg cagatcatct atgccgggta
     2761 cgtccggccg acggccgaag cgtggcgcgc cgagcagcgc gcgatcgcgg cgcggacccc
     2821 cggctacacg ccggagcggt ccatctacgt catcatccaa gaccccgagc agcaagcgac
     2881 gatcatcgtc tgcatctggg cgatgatcct gtcagtcatg cgcttccacg aaattcgcgc
     2941 gcaacgcgag ctgttgacgg ccggctactt cacgaaggac gccaacaccg tgatcttgc
//
LOCUS       DATLEE010000265         2362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8347183, whole genome shotgun sequence.
ACCESSION   DATLEE010000265 DATLEE010000000
VERSION     DATLEE010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8347183"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..461)
                     /locus_tag="VM692_05520"
     CDS             complement(165..461)
                     /locus_tag="VM692_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51658.1"
                     /translation="MTNELPVATQSPNWQREATIALAAIGFGLLILPFAVYVVGSELI
                     GVYDPDGGGALDLAETIWLDLLSLRLPAWVLVLSPYITVQLARWVRRVWRPKTL"
     gene            complement(468..2123)
                     /locus_tag="VM692_05525"
     CDS             complement(468..2123)
                     /locus_tag="VM692_05525"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUQ51659.1"
                     /translation="MSKPSSEDLDNDHTDELPVLLETVALGDEPLFGPAREDTSEHTA
                     LYDASALPSADVAALHDELAEHVMRSAQLQAEVRTLANRARELERHAAEKDQLIGELE
                     HTVATLRAAADETSSAEHRLAEQLAVRDARIAEAATTVEQLQRESAARSAEIEQLRTA
                     AATLRREADAAKLELAARPPANPALQDAQLLEDNAALRDYIAGRQAWWNGQQAKQASL
                     TAKLTALQRELATSKKELATAEALATRESSRAVALRAELVDHARRVEELERQLRAQRA
                     VITDSAPTHAPAAGPAPPAPPSPPIRVEGVPAPAAVPESPAAADAISTDVPAVEAIAQ
                     LEAEVGYKRQQVAAQLVELRERDQRLQGAAVDLERLRRELSAVRNELDESRSAVARLE
                     RAVIDKDRALEARDARIATLHEELKQRLGAIEKLNAIDFSMPKGDSPIVTRAAAIETA
                     GESPAPALACLTGDAPKRFALTKKTTTVGRGQHCDLQILTHFVSREHARITVANGTVL
                     IEDLGSRNGVYVNAVRVDRHALQQGDLITIGETQFRFMESMAH"
BASE COUNT          361 a          776 c          792 g          433 t
ORIGIN      
        1 gagcagcccc gagtcggagt cccagcctaa ccagcgtttc aagcgcttca gcatttaacg
       61 atccaaagtg cgcgtcgacg tgcgtgcgca cgttcgcgtg gcaaaaaacg atagcagatg
      121 tggccgcgtg gccagggaaa cggctgcaga ttctgtgacg gcaatcacag cgtcttcggc
      181 cgccaaacgc ggcgcaccca ccgcgccaat tgcacggtga tgtaagggct caaaacgaga
      241 acccaggccg gcaatcgtaa cgacaggaga tcaagccaga tcgtttcggc gaggtcgagc
      301 gcgcctccgc catcgggatc gtaaacgccg atcagctcgc taccgaccac ataaacggca
      361 aacggcagga tcagcaaccc gaaaccgatc gccgctaagg caatcgtggc ttcgcgctgc
      421 cagttcggtg actgcgtagc aaccggtagt tcgttggtca cggtgcgtta gtgtgccatc
      481 gattccatga agcgaaactg cgtctcgccg atcgtgatga ggtccccttg ttgtagcgcg
      541 tggcgatcga cgcggactgc gttgacgtag acgccattgc ggctgcccaa gtcttcgatc
      601 agcacggtgc cgttggccac tgtgatccgc gcgtgctcgc gactgacgaa atgcgtgagg
      661 atttgcagat cgcagtgctg gcctcgcccg accgtcgttg ttttcttggt cagcgcgaac
      721 cgcttcggcg cgtctccggt taggcaagcc aatgcgggtg ccgggctttc gccggcggtt
      781 tcgatcgccg ctgcccgcgt cacgatcggc gagtcgcctt tcggcatcga gaaatcgatt
      841 gcattgagct tctcgatggc gccgaggcgt tgcttgagct cttcgtgcaa tgtcgcgatt
      901 ctggcgtcgc gcgcctcgag cgctcgatcc ttgtcgatca cagcacgttc gagccgcgcg
      961 acggccgagc gactctcgtc gagctcattg cgcaccgccg acaattcgcg ccgcagccgc
     1021 tcgaggtcca cggcagcgcc ctgcagccgc tggtcgcgct ctcgcagctc gacgagctgc
     1081 gcggcgactt gctgacgctt gtagccgact tcggcctcga gctgggcgat ggcttcgacg
     1141 gcaggaacgt ccgtgcttat ggcatccgcg gcggccggcg actctggaac cgcggcagga
     1201 gcgggcaccc cctcgacgcg gatcggcggc gacgggggag cgggcggggc cggtccggcc
     1261 gccggcgcgt gcgtaggcgc agaatccgtg atgaccgcgc gctgcgcgcg cagctggcgc
     1321 tcgagctcct cgactcggcg cgcatggtcg acgagctcgg cgcgcagggc gacggcacgg
     1381 ctcgactcgc gcgtcgcgag agcctcggcc gttgcgagct ccttcttgct ggtcgcgagc
     1441 tcgcgctgca atgccgtcag cttcgccgtg aggctcgcct gcttcgcctg ctgcccgttc
     1501 caccacgctt gccgacctgc gatgtagtca cggagcgcgg cattgtcctc gagcagctgc
     1561 gcgtcctgca gcgcggggtt cgcaggaggc cgcgcagcga gctcgagctt cgcagcgtcg
     1621 gcctcgcggc gaagcgttgc cgcggctgtg cgcaactgct cgatctcggc cgagcgtgct
     1681 gcggactcgc gctgcagctg ctcgaccgtc gtagcggcct ctgcaattcg cgcgtcgcgt
     1741 acggccaact gctccgcgag gcgatgttcc gccgagctcg tctcgtccgc ggctgcgcgc
     1801 agcgtggcga ccgtgtgctc gagctcgcca atcagctgat ccttctcggc tgcgtgacgc
     1861 tccagctcgc gcgcgcggtt cgcgagtgtg cgaacctcgg cttgcagttg agcactccgc
     1921 atgacgtgct cggcaagctc gtcgtgcagc gccgcaacgt cggcgctcgg aagcgcgctc
     1981 gcgtcgtaca gcgcggtgtg ctcggaggtg tcctctcgtg ccgggccgaa caacggctcg
     2041 tcaccgagcg cgaccgtctc gagcaggacc ggcagctcgt cggtgtgatc gttgtcgagg
     2101 tcttccgagg atggtttaga cattcgagga acttacgaac gtttgctcgg ctaatacgtt
     2161 aggcggtttc gagccacgcg gctagccggc gtcactccgc cggcaccgtg cggccggcgg
     2221 cccaggcgcg cagtgccgtc acgcgctcgg ccatgagcac ggacagtggg cgcgtcttct
     2281 tgagctcgag tgccagatgg ccgtgatcgg gttcttgacc cagcgcgtgc gccgcgtata
     2341 gggacgagac gatcccttgc tc
//
LOCUS       DATLEE010000266         4825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9241197, whole genome shotgun sequence.
ACCESSION   DATLEE010000266 DATLEE010000000
VERSION     DATLEE010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9241197"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..898)
                     /locus_tag="VM692_05530"
     CDS             complement(35..898)
                     /locus_tag="VM692_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ51660.1"
                     /translation="MSDARPEILHAPTSDTSGFAVTAQPLGFWTKLYDNGTVRKTILL
                     ILLAAAWEIYARALANPLLFPTFTATFGALLSGIADGEIPTAGLYTLSLLLKGYVAGL
                     FFAALLTAFASVSRIGADLLETLTAMFNPLPSIALLPLALIWFGLGDNSVIFVLVHAV
                     LWAVALNTFAGFRALSPTLRMVGQNYGLTSVGYIVRILIPGAFPSILTGLKVGWAFAW
                     RTLIAAELVFGVSSGSGGLGWYIFEKKNELRIPEVFAGLLTVIIFGLFVENVIFRTIE
                     RRTVKRWGMQT"
     gene            complement(891..1664)
                     /locus_tag="VM692_05535"
     CDS             complement(891..1664)
                     /locus_tag="VM692_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUQ51661.1"
                     /translation="MQQSALLSVNGVTLQYRTGEQLVTATYRVSFDVLQGDRFVVLGP
                     SGCGKSTLLKAIGGYLTPVEGEMLLNGARIGKPGPDRAFVFQEFDQLLPWKTVRDNVV
                     FALTASGKLAKKAATERAHHFIDKVGLGKFADNFPHTLSGGMKQRVAIARAMATEPQI
                     LLMDEPFAALDALTRGKMQEDLLQLWEDTRFTVVFVTHSIPEAIRIGNRILLLTPHPG
                     RTKAELNCTGADVASDGGELLSARIQRLLFDRTGAIADV"
     gene            1745..2794
                     /locus_tag="VM692_05540"
     CDS             1745..2794
                     /locus_tag="VM692_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HUQ51662.1"
                     /translation="MSLGTHEKPPHAILFEMLFGAWTAKILAEVVRLDVPDALVDGPM
                     TADELVRTAGIRAHPVALHRALRACAALGIVTEDSVGRFGPTRLSALLTRDTPGTLKR
                     FVEYSGGTLWKVWSGLAEGLATGASQARAQLGMEFFDYLQANPQALEEFGETLKAHSA
                     VANRGVIERYDFGGIRTLVDVGAGYGPLTMAVLEKYPDIRGVVFDLPEVIDAAPSQLP
                     VKNESVARRLSYVAGDMFKDVPPADAFALKLILHDWDDAQCATILANVCARLERGGRV
                     ISIDNVMPPLGDASDVPQKLLDVNMMLLLPGKERTRVEWEALYRGAGLAITAMVPVPD
                     AFNMFVIEGRRVDER"
     gene            complement(2801..4372)
                     /locus_tag="VM692_05545"
     CDS             complement(2801..4372)
                     /locus_tag="VM692_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HUQ51663.1"
                     /translation="MLYRFAAFELDLAAVELRASGSPVKLEPQVFALLALLVANGERL
                     VSKDEIIEKVWDGRVVSDSAVASRVKSARAALGDDGNAQKFIKTVHGQGYRFVGEARA
                     ARSRASAEPGDASADSLAGLVQGLERVARPSLAVLPFRFVGADQRSAALATALPDELI
                     TELARLRWLFVTARGSSFRLRAPDADFGEIGRLLRVRYCLWGTVELSASRLAVMVELV
                     DTNDGGIVWAERFSGRLDDVHTMREQIRSQVLMALELRIPLHEASLARVSAVENLDAW
                     SAYHLGLQHLYRFNRHDNAAAANLFQRAVALDPTFARAHAGMSFVHFQGAFMHYTDEL
                     ARAIAEARRCAERGLELDPLDPFVNFTMGRTYWLDGDLETSLGWLERATQISPNYAHG
                     IYAQAWTEVMAGRSTESRGHVDHALRLSPLDPLHYAMLATRGFTHLLRGEDAEAARWA
                     ERGARSPGAHVLIALIAAASHALCGDNARAAFWAANVRERNPALTQADFFRAFPIKSD
                     AMRARLERGLALVGF"
     gene            4521..4745
                     /locus_tag="VM692_05550"
     CDS             4521..4745
                     /locus_tag="VM692_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HUQ51664.1"
                     /translation="MPLVTIDVIKDVFTPSQKKELIAKVTEAMIAVEGENMRPVTWVR
                     ISEFEGGDWAIGGKALKAADVHALAAGKAA"
BASE COUNT          851 a         1555 c         1648 g          771 t
ORIGIN      
        1 gagagccgcg ccgcgttgcg atgcaggtta tcgcttacgt ctgcatgccc caccgtttaa
       61 cggtgcggcg ttcgatggtg cggaagatta cgttttcgac gaacaggccg aagatgatga
      121 cggtcagcag gccggcgaag acttcgggga tgcggagctc gtttttcttt tcgaagatgt
      181 accagccgag gccgccggag cccgagctga cgccgaagac gagctcggcg gcgatgagtg
      241 tcctccacgc gaacgcccag ccgaccttca gtcccgtcag gatgctcgga aaggcgcctg
      301 gtatcaggat ccgcacgata tagccgacgc tggtgaggcc gtagttctgg ccgaccatgc
      361 gtagcgtcgg tgatagcgcg cggaagcctg cgaacgtgtt caacgcgacg gcccacagca
      421 ccgcgtgcac gagcacgaag atgacgctgt tgtcgcccaa gccgaaccag atcaacgcga
      481 gcggcagcaa cgcgatcgac ggcagcgggt tgaacatcgc cgtcaacgtt tcgagcaggt
      541 cggcgccgat gcgcgagacg ctcgcgaacg ccgtcagcaa ggctgcgaag aacagtccgg
      601 cgacataccc tttcagcagc agcgacagag tgtagagacc ggcggtcgga atctcaccgt
      661 cggcgatgcc ggacagcagc gcgccgaagg tagccgtaaa cgtcggaaac agcagcgggt
      721 tcgcgagcgc gcgcgcgtag atttcccagg cggcggccaa caagatcagc agaatggtct
      781 tgcgcaccgt gccgttgtcg tagagcttcg tccagaagcc gagcggctgc gccgtgacgg
      841 cgaagccgct cgtgtcgctc gtcggcgcgt gcaagatttc agggcgagcg tcagacatcg
      901 gcgatggctc ccgtgcgatc gaacaacagg cgctgaatgc gcgccgacaa cagctcgccc
      961 ccgtcgctcg cgacgtccgc gcccgtgcaa ttgagctcgg ctttcgtacg gcccggatgg
     1021 ggcgtcagca gcaggatccg attaccgatg cgaatcgcct cggggatcga atgcgtgacg
     1081 aacacgaccg tgaagcgcgt gtcctcccag agctgcagta ggtcttcctg catcttgccg
     1141 cgcgtcagcg cgtcgagtgc cgcgaacggc tcgtccatga gcaggatctg cggctccgtg
     1201 gccatcgcgc gcgcaatcgc gacgcgctgc ttcataccgc ctgacagcgt gtgcgggaag
     1261 ttgtcggcaa atttgccgag gccgaccttg tcgatgaagt ggtgcgcgcg ctcggtcgcc
     1321 gctttcttgg cgagcttgcc gctcgccgtc agcgcgaaga cgacgttgtc gcgcaccgtc
     1381 ttccagggca gcagctggtc gaactcttgg aacacgaacg ctcgatcggg cccgggtttg
     1441 ccgatgcgcg cgccattgag cagcatctcg ccctcgacgg gagtcagata gccgccgatc
     1501 gctttcaaca gcgtcgattt gccgcagccc gaaggtccaa gcacgacgaa gcgatcgcct
     1561 tgcaggacgt cgaagctgac tcggtatgtc gccgtcacga gctgctcgcc cgttcggtac
     1621 tgcaacgtga cgccgttgac ggagaggagc gctgactgct gcatggcgcg gcgagtatac
     1681 cttgggctcg agtcgtggca gagtcgcgcg agttcgatcg caacgccgag caagagcatc
     1741 gagagtgagc ttaggaacgc acgaaaaacc gccgcacgcg atcttgttcg aaatgttgtt
     1801 cggcgcttgg acggcaaaga tactggccga ggtcgtgcgg ctcgacgtgc ccgatgcgtt
     1861 ggtcgacggg ccgatgacgg ccgacgagct cgtgcgcacg gccggcatcc gcgcgcaccc
     1921 cgtcgcgttg caccgcgcgt tgcgggcgtg tgcggcgctc ggcatcgtca cggaggactc
     1981 cgtcggccga ttcggcccga cacgcttgtc ggcgttgttg acgcgcgata cgccgggcac
     2041 gctgaagcgc ttcgtcgagt attcgggcgg cacgttgtgg aaggtctgga gcggtttggc
     2101 cgagggcctc gcgaccggtg cgagccaagc ccgcgcgcag ctcggcatgg agttcttcga
     2161 ctacctgcag gcgaatccgc aagcgctcga agaattcggc gaaacgctga aggctcacag
     2221 cgccgtcgct aatcgcggcg tcatcgagcg ctacgacttc ggcggcatcc gcacgctcgt
     2281 ggatgtcggc gcgggttacg ggccgctgac gatggccgtg ctcgagaagt atccggacat
     2341 ccgcggcgtc gtgttcgatc tgcccgaggt catcgatgcc gcgccgtcgc agctgccggt
     2401 gaagaacgaa agcgttgcgc gccggctcag ctacgtcgcg ggcgacatgt tcaaggacgt
     2461 gccgccggcc gatgcttttg cgttgaagct gatcctgcat gactgggacg acgcgcagtg
     2521 cgcgactatc ctcgcgaacg tttgcgcgcg cctagagcgc ggcggccgcg tcatctcgat
     2581 cgacaacgtc atgccgccgc tcggcgacgc gtccgacgtc ccgcagaagc tgctcgacgt
     2641 caacatgatg ttgttgttgc ccggcaagga gcgaacgcgc gtcgagtggg aggcgctgta
     2701 tcggggcgcc ggcctcgcca tcacggcgat ggtccccgtc cccgacgcgt tcaacatgtt
     2761 cgtcatcgaa ggccgacgcg tcgacgagcg ctagcactgt tcagaagccc acgagcgcaa
     2821 gcccgcgctc gagtcgcgct cgcatcgcgt cggacttgat cgggaaggcc cggaaaaaat
     2881 cggcttgcgt caacgcggga ttgcgctctc gcacattcgc tgcccaaaaa gccgcgcgcg
     2941 cattgtcgcc gcagagcgcg tgcgacgccg ccgcgatcaa ggcgatcaag acgtgtgcac
     3001 cgggcgagcg cgcgccgcgc tctgcccagc gtgcggcttc ggcgtcctcg ccgcgcagca
     3061 ggtgcgtgaa gccgcgcgtc gccagcatcg cgtagtgcaa cggatcgagc gggctcagcc
     3121 gcagcgcatg gtcgacgtgc ccacggctct cggtggagcg gccggccatg acctcggtcc
     3181 aggcctgcgc gtagatgccg tgcgcatagt tcgggctgat ctgcgtcgcg cgctctagcc
     3241 aaccgaggct cgtctcgaga tcgccgtcga gccaatacgt gcggcccatc gtgaagttga
     3301 cgaatggatc gagcgggtcg agctcgagcc cgcgctcggc gcagcgccgc gcctcggcga
     3361 tcgcgcgtgc gagctcgtct gtgtagtgca tgaaggcgcc ctggaagtgc acgaacgaca
     3421 ttccggcatg ggcgcgcgca aacgtcgggt ccagcgcgac ggcgcgttgg aacagatttg
     3481 cggccgcggc gttgtcgtgg cgattgaagc gatacaagtg ctgcaggccc aggtggtatg
     3541 cggaccatgc gtcgaggttc tcgacggcgg agacgcgagc cagcgacgcc tcgtgcagcg
     3601 gaatgcgaag ctcgagcgcc atcaagacct gcgagcggat ttgctcgcgc atcgtgtgca
     3661 cgtcgtcgag ccgcccgctg aagcgctcgg cccagacgat gccgccgtcg ttcgtgtcga
     3721 cgagctcgac catgaccgcg agccgcgaag ccgagagctc gaccgtgccc cagaggcaat
     3781 accgcacgcg cagcaggcgg ccgatttcgc cgaagtccgc gtcgggcgcg cgcagccgga
     3841 acgacgagcc gcgcgccgtg acgaacagcc agcgcaagcg ggcgagctcc gtgatcagct
     3901 cgtccggcag cgccgttgcg agcgccgcag agcgctggtc ggcgccgacg aagcggaacg
     3961 gcaacacggc cagcgacggc ctcgcgacgc gctcgaggcc ttgcacgagc cccgcgagac
     4021 tgtcggcgct cgcgtcgccc ggctccgcgg acgcgcggct gcgcgcggcg cgcgcctcgc
     4081 cgacgaagcg atagccctgg ccgtggaccg tcttgatgaa tttctgcgcg ttgccgtcgt
     4141 cgccgagcgc ggctctcgcg gacttcacgc ggctcgcgac ggccgaatcc gacacgacgc
     4201 gcccatccca gaccttctcg atgatctcgt ccttcgagac gagccgctcg ccgttcgcga
     4261 cgagcagcgc gagcagggca aacacctgcg gctcgagctt cacgggcgag ccggacgcgc
     4321 gcagctcgac ggcagcgagg tcgagctcga aagcggcaaa tcggtagagc atgcgccgcc
     4381 taggataaat cagggcgcaa cttccaaacg atctccacga tctctccatg cggtctgcaa
     4441 gcttgaggtt gcacggcgtg cgaacctttt ccgcactgag cttgggtggt ccgagctcgc
     4501 acaaccgaaa ggagagagtc atgccattgg tcaccatcga cgtcatcaag gacgtattca
     4561 ccccgtcgca gaagaaggag ctcatcgcga aggtcacgga ggcgatgatc gccgtcgagg
     4621 gcgaaaacat gcgccccgtc acgtgggttc gcatcagcga gttcgagggc ggcgactggg
     4681 ccatcggcgg caaggcgctg aaggcggccg acgtccacgc gcttgcggcc ggcaaagctg
     4741 cgtagcaggc ctcgggcggg tcgcagcggc ggcccgcccg cagtcccggc cactttgacg
     4801 ggttcaccgc tggcaggggc tcagc
//
LOCUS       DATLEE010000267         5848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1793667, whole genome shotgun sequence.
ACCESSION   DATLEE010000267 DATLEE010000000
VERSION     DATLEE010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793667"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VM692_05555"
     CDS             complement(<1..324)
                     /locus_tag="VM692_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51665.1"
                     /translation="MLTVVLTGLYVRSPSSRRSAEAPAVALTIGEPHTINLVFGARAA
                     LGDVEFIVDLPPGIELADHAGERRVTGRAALTAGDNALPVTVVARDGTGGQLAARLRH
                     GDQQKT"
     gene            556..1353
                     /locus_tag="VM692_05560"
     CDS             556..1353
                     /locus_tag="VM692_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HUQ51666.1"
                     /translation="MRAMIALAALVFAGEAAARDLQDVLNTGTLRVGVTLFAPWAARG
                     ANGELVGFEVDVAKQLAADMGVKPQLLAYDIDRLVPAIEAGEIDVIAAGMTITPDRAL
                     HVNFSQPYAESGIALATHVASTAKVTDIGALNGDEYTIAAVEGSVGLQLARRLWPRAR
                     VQTFASVAAATEALLAGEVHGYVEDEPVPTFLALENPGVIDVPTTRPLLASRAGFAVA
                     KGDADFIAYLNAWITAHEADTWLPTTVSYWFKSLRWRERLGDATSRR"
     gene            1355..1840
                     /locus_tag="VM692_05565"
     CDS             1355..1840
                     /locus_tag="VM692_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51667.1"
                     /translation="MRVGVLVAALTATAQPLAAQSPDAPRAPRIIPAPGAPAPATPTP
                     APATTTREPPLQLPEVTLPEGRRPPNAASLAPLPDERKGEPEEIIVIGQGWRLPDLGS
                     EWRAKQKEAQREGGFSATALPLYDPAEPPLHSEAFTSREQRRHGYIELFRLRFGRRSR
                     D"
     gene            complement(1848..3398)
                     /locus_tag="VM692_05570"
     CDS             complement(1848..3398)
                     /locus_tag="VM692_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51668.1"
                     /translation="MRALRAWLVRAAAAAAALPVCAQDEAEVVSPRADSIAVTIYRDD
                     LALITETRTVDLPAGGVTLVFEGVVETLLPQSAIVTDVARPLAESNFDFDRLTPRSLL
                     ERSIGETVTITRTNPATGRVTQTAATILAVGDGVVLRTTDGNEALYCSGLPERLELMR
                     IPDELLGKPRLSVRLAAGEAGARTVKISYLAHGFAWSSDYVAHLNERSDAMRLAGWAT
                     LTNSTGTVFEQADVQLVAGRLNILVAEVGGSAAPIDVVDEVDKANAEVATLRECFATD
                     LPVPQLLERMQTIMFDRAAPQAALSSLEEITLTASRAVREELGDYQLYRLPWRADLNA
                     RQTKQVLFLDEPAIRIERFYGLRLESLTEPLPQDVAIPNLIVRFENTEAQGLGEPLPR
                     GVVRAFETFDGREVFAGEAEIADRPVGLPVELVLGRALNVMLEVTTSPSRNGARVAAT
                     TEHVIVNNKSVPIDLEIRHAVESYYTDVELESTSRPMRRKYGDLAWRFDVPPGEEVLR
                     YRLSARSP"
     gene            complement(3395..4825)
                     /locus_tag="VM692_05575"
     CDS             complement(3395..4825)
                     /locus_tag="VM692_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HUQ51669.1"
                     /translation="MRRNAWISASLASLAAATAGAQPVAADKSLALTIYNSDLALVEH
                     VRPLTVAAGIHRLEFQGVSAQIMSQTVSFAASGLTVIEQNFDYDLLTPEKLMEKAVGQ
                     TVRIVRTNPGTGRETEETAEVLSTVGGVVLKIGERIEVLREDGIPARVIFDEVPPNLR
                     ANPTLSILANAERPVNGDVRLAYLTHGLSWNADYVAVFDEPAGNVSMQGWITLTNNSG
                     TTFANANAQLVAGDVNVVGSEAEWWQVYNVRRDRPPFGDYQGAPNVLRAGGVESSNRE
                     QLADYYVYPLGAATTIANNQTKQVSFLTAERVRANRGYEQTFYSFYSQEEPVSAEVRV
                     RFSNSQASGLGDQLPSGVVRVYARDARGQPQFVGEDRIGHTSAGSDLALKIGDAFDVT
                     IQPTLRQTTRSSRRATDYAMSYLVRNARSEPITVTIRQDNLWRFNEVTAQSIQGRRTD
                     ADSFAWDVPVPANGETELTFTIRQSW"
     gene            4940..5701
                     /locus_tag="VM692_05580"
     CDS             4940..5701
                     /locus_tag="VM692_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HUQ51670.1"
                     /translation="MLDWPALLLIQGFQMDGAVLVIHDEQALRAAARHALTQAGRSVA
                     EAAEVSAAFSIADRVRPAAIVLPWLGRRPAREALAQLRDTDATRRSRLIVWAEHSDMP
                     NAVAALEFGADDCLALPFEPSELVARINACLRRPLAAIRPDQLTAGPLVLDKAVHRLL
                     VRGKEVSLSPAEFRLLAFLLENQGRVYSREELLRGAWPKNISAGGRTVDVHVRRLRQL
                     LEPFGCDDLIQTVRSFGYRLALPSSEPRVVAATFL"
BASE COUNT          930 a         2023 c         1956 g          939 t
ORIGIN      
        1 agtcttttgc tggtcgccgt gacgcaggcg cgccgcgagc tggccgccgg tgccatcgcg
       61 cgccacgacg gtcaccggca aggcgttgtc gccggccgtc agcgccgcgc gacccgtgac
      121 gcgacgctcg ccggcatggt cggcgagctc gataccggga ggtagatcga cgatgaactc
      181 gacgtcgccg agcgccgcgc gcgcgccgaa aacgaggttg atcgtgtgcg gctcgccgat
      241 cgtcaacgcg acggccggcg cttcggcgct gcggcgcgag ctcgggctgc gcacgtacaa
      301 gcccgtcaag acgaccgtca gcatcgtcag cccgaacgcg ccgaggaagg tgaacgcaac
      361 gatgcgctgt gcaagagtcg gtttcggtct tcgcggcgcg tcaggcgagc tcgtatcggt
      421 cggcgtcgtc gtcacggtcg gtcggcttcg gcagttcgat cggttacgat gcccaaggat
      481 accctgaatg cggccgaacc aaccgcggcc tgcaccggtc acaagaccgt aagcgcagca
      541 ggcggcagag tacgtatgcg agcgatgatt gccttggcag ccctggtgtt cgccggcgaa
      601 gccgccgcgc gcgacctgca agacgtgctg aataccggca cgctgcgcgt cggagtgacg
      661 ctgttcgcgc cctgggccgc acgtggtgcc aacggcgagc tcgtcggctt cgaggtcgac
      721 gtcgcgaaac agctcgcggc cgacatgggc gtcaagccgc agctgttggc ctacgacatc
      781 gatcggctcg ttcccgcgat cgaagccggc gagatcgacg tcatagcagc cggcatgacg
      841 atcacgccgg accgcgcgct gcacgtcaac ttcagccagc cgtatgccga gagcggcatc
      901 gcgctcgcga cgcatgtcgc gagcacggcg aaggtcacgg acatcggcgc gctcaacggc
      961 gacgagtaca cgatcgcggc cgtcgaaggc tccgtcggct tgcagctcgc acggcgcttg
     1021 tggccgcgtg cccgcgtgca aacgttcgcg tccgtcgccg ccgccaccga agcactgctc
     1081 gcgggcgagg ttcacggcta cgtcgaagac gagcccgtgc cgacgttctt ggcgctcgag
     1141 aaccccggcg tcatcgacgt gccgacgacg cggccgctgc tcgcgagccg cgcgggtttc
     1201 gccgtcgcca aaggcgatgc cgacttcatc gcctatctca atgcgtggat cacggcgcat
     1261 gaagccgaca cgtggctgcc gacgacggtc agctattggt tcaaatcgtt gcgctggcgt
     1321 gagcgactcg gcgatgcgac ctcgcgccgc tagcttgcgc gtcggagtgc tcgtcgccgc
     1381 gctgacggcg accgctcagc cgctcgcggc gcagagccct gacgcaccgc gcgcgccgcg
     1441 catcatcccc gcgcctggcg cccctgcgcc ggcgacaccg accccggcgc ctgcgacgac
     1501 gacgcgcgag ccgccgctgc agcttcccga ggtgacgttg cccgagggcc ggcgcccgcc
     1561 gaacgcggcg agcctcgcgc cgctgcccga cgagcgcaaa ggcgagcccg aggagatcat
     1621 cgtcatcggc caaggctggc ggctgcccga tctcggcagc gaatggcgcg caaagcagaa
     1681 ggaggcgcag cgcgaaggcg gcttcagcgc gacggcgctg ccgctgtacg accccgccga
     1741 gccgccgctg cacagcgaag ccttcacgag ccgcgagcag cgccggcacg gctacatcga
     1801 gctgttccgc ttgcgcttcg gccgccgctc gcgcgactga cgccgcgtta cggcgagcgc
     1861 gccgacaagc ggtagcgcaa cacttcctcg ccgggcggca cgtcgaaccg ccacgcaaga
     1921 tcgccgtatt tgcggcgcat cggccgcgag gtgctctcga gctcgacgtc cgtgtagtag
     1981 ctctcgacgg cgtggcgaat ctcgagatcg atcggcacgc tcttgttgtt gacgatgacg
     2041 tgctccgtcg tcgcggcgac acgcgcgccg ttgcggctcg ggctcgtcgt gacctcgagc
     2101 atgacgttca gcgcgcggcc gagcacgagc tcgacgggca agccgacggg ccgatcggcg
     2161 atctcggcct cgcccgcgaa cacctcgcgg ccgtcgaaag tctcgaaagc acgcacgacg
     2221 ccgcgcggca acggctcgcc gagaccttgg gcctccgtgt tctcgaagcg cacgatgagg
     2281 tttgggattg cgacgtcctg cggcagcggt tccgtcaagc tctcgagccg caggccgtag
     2341 aagcgctcga tgcggatggc cggctcgtcg aggaacagca cctgcttcgt ctgacgcgca
     2401 ttcaaatcgg cgcgccacgg cagtcggtag agctgatagt cgccgagctc ctcgcgcacg
     2461 gcgcgactgg ccgtgagcgt gatctcctcg agcgaggaca aagcggcctg cggcgcggcg
     2521 cgatcgaaca tgatggtctg cattctttcg aggagctgcg gcacgggcaa atcggtcgcg
     2581 aagcattcgc gcagcgtcgc cacctccgcg tttgccttgt ccacctcatc gactacgtcg
     2641 ataggcgcag cgctgccgcc tacctcggcc actaggatgt tgagccggcc cgcgacgagc
     2701 tgcacgtcgg cctgctcgaa gaccgtgccc gtcgagttcg tcagcgtcgc ccaacccgcg
     2761 aggcgcatcg cgtcgctgcg ctcgttcaag tgcgcgacgt aatccgaaga ccatgcaaag
     2821 ccgtgcgcga gatagctgat cttgacggtg cgggcgccgg cttcgccggc cgcgagccgc
     2881 accgacaaac gcggcttgcc gagcagctcg tccgggatgc gcatgagctc tagccgctcg
     2941 ggcagacccg agcaatacaa tgcctcgttg ccgtcggtcg tgcggagcac gacgccgtcg
     3001 ccgacggcca ggatcgtcgc cgccgtttgc gtcacgcggc ccgtcgcagg attcgtgcgc
     3061 gtgatcgtca cggtctcgcc gatcgaacgc tcgagcagcg accgaggcgt cagccgatcg
     3121 aagtcgaaat tgctctcggc caacggtcgc gcgacgtcgg tcacgatcgc cgactgcggc
     3181 aacaacgtct cgacgacgcc ctcgaacacg agcgtgacgc cgccggccgg caggtccacg
     3241 gtgcgcgtct cggtgatcag cgcaagatcg tcgcgataga tcgtcacggc gatcgagtcc
     3301 gcgcgcggcg agacgacctc ggcctcgtcc tgcgcgcaca cgggcagcgc cgccgctgca
     3361 gcggcagccc gcacgagcca tgcgcgtagc gccctcacca gctctgccga atggtgaacg
     3421 tgagctcggt ctcgccgttc gcgggcacgg gcacgtccca ggcgaagctg tcggcgtcgg
     3481 tgcgccggcc ctgaatcgat tgcgccgtga cctcgttgaa gcgccagagg ttgtcctggc
     3541 ggatcgtcac ggtgatcggc tccgagcgcg cattgcgcac gagatacgac atcgcgtagt
     3601 cggtcgcgcg ccgcgacgag cgcgtcgtct gcctgagcgt cggttggatc gtcacgtcga
     3661 acgcgtcgcc gatcttcagc gcgaggtcgg agcccgcgct cgtgtggccg atgcggtcct
     3721 cgccgacgaa ttgcggctgg ccgcgtgcgt cgcgcgcgta gacgcgcacg accccgctcg
     3781 gcagctgatc gccgagcccg ctcgcttgcg agttcgaaaa ccgtacccgg acctcggccg
     3841 agaccggctc ttcttgcgag tagaacgaat agaacgtctg ctcgtagccg cggttggcgc
     3901 gcacgcgctc ggccgtcaag aagcttactt gcttcgtctg attgttcgcg atcgtcgttg
     3961 cggccccgag cgggtaaacg tagtagtcgg cgagctgctc gcggttcgac gactcgacgc
     4021 cgccggcgcg cagcacattg ggcgcccctt ggtagtcccc gaaggggggg cggtcgcggc
     4081 gcacgttata gacctgccac cactcggctt cgctgccaac gacgttgacg tcgccggcga
     4141 cgagctgggc gttcgcgttg gcgaacgtcg tgcccgagtt gttcgtcagc gtgatccagc
     4201 cctgcatcga gacgttaccg gccggctcgt cgaacacggc gacgtagtcg gcgttccacg
     4261 agaggccgtg ggtcagatag gcgagccgta cgtcgccgtt cacgggccgc tccgcgttcg
     4321 cgagaatcga caacgtcgga ttcgcgcgca gattcggcgg cacctcgtcg aagatgacgc
     4381 gcgccggaat gccgtcctcg cgcagcactt cgatgcgctc gccgatcttc agcacgacgc
     4441 cgccgaccgt cgacaagacc tcggccgttt cctcggtttc gcgccccgtg ccgggattcg
     4501 tgcgcacgat ccgcacggtc tggccgacgg ccttctccat gagcttttcc ggagtcagca
     4561 ggtcgtagtc gaagttctgc tcgatgaccg tcaggccgga cgcggcgaag ctcacggtct
     4621 gcgacatgat ctgagccgac acgccttgga actcgagccg atgtatgccg gcggcgaccg
     4681 tcagcggccg cacgtgctcg acgagcgcta gatcggagtt gtaaatcgtg agcgccaagc
     4741 tcttgtcggc tgcaaccggc tgcgcgcccg cagtcgcggc cgccagcgaa gccaaacttg
     4801 ctgagatcca agcgttacgc cgcatgtggc cctcctggct atcgtcgttg gcgagcatta
     4861 tcggggcgcg tgtgccaatg cgcgacggca gtcttgtaaa aaaaccgtaa cggtgacgta
     4921 aaacaactgc aaaccgcccg tgctggactg gcccgcactc ctcttgattc aaggcttcca
     4981 gatggacggc gcggtactgg tgatccacga cgagcaggcc ctgcgcgccg cggcgcgcca
     5041 cgcgttgacg caagcgggcc gctcggtcgc cgaagcggcc gaggtttccg ccgcgttttc
     5101 gattgccgac cgcgtgcggc ccgcggccat cgtgctgccg tggctcggcc gccgccccgc
     5161 gcgcgaggcg ctcgcgcagc tgcgcgatac ggacgcgacg cgccgcagcc ggctcatcgt
     5221 ctgggccgag cacagcgata tgccgaacgc cgtcgccgcg ctcgagttcg gcgccgacga
     5281 ttgccttgcg ttgccgttcg agccgtcgga gctcgtcgcg cgcatcaacg cgtgcttgcg
     5341 gcggccactc gccgcgattc gccccgatca actgacggcc ggcccgctcg tgctcgacaa
     5401 agccgtccac cggctgctcg tgcgcggcaa ggaagtctcg ctgtcgccgg ccgaattccg
     5461 cttgctggcg ttcttgctcg agaaccaagg gcgcgtgtac agccgtgaag agctactgcg
     5521 cggcgcttgg cccaagaaca tctcggccgg cggccgcacg gtcgacgtgc acgtgcgtcg
     5581 cctgcgccaa ttgctcgagc cgttcggctg cgacgaccta atccagaccg ttcgcagctt
     5641 cggctatcgc ctcgcgctgc cgagctcgga gccgcgcgtc gtagccgcaa cattcctgta
     5701 accaaaaatc cacggttctg caatcaacga cgcctagagt cgcggcttcg aacacgaacg
     5761 cgcccctcgg acttcccgga caggagatcg aacaatgagt cttaccttca tgtcgcgcac
     5821 ccgattgatg gttggcggca gccttttg
//
LOCUS       DATLEE010000268         6442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3141282, whole genome shotgun sequence.
ACCESSION   DATLEE010000268 DATLEE010000000
VERSION     DATLEE010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3141282"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VM692_05585"
     CDS             complement(<1..1329)
                     /locus_tag="VM692_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ51671.1"
                     /translation="MRNRQVVVFALSLLCGVGARAGEGAGSLLDAAKHGTTMELRGLL
                     RSEVDPNAADADGTTALHWAVHRGDLEGVRALLTAGAAADAANRYGVRPAYLAAENGD
                     AAALRALLEGGADPRATFAEGETLLMTAARTGAVATIELLVAAGADVNAAEERGGQTA
                     LMLAAAENNGPAIAALIKAGADRDAREATGEFTALAFAVRADAVVAVKALLEAGADAS
                     GALPDGTSMIVLAALNQNYEAASALLDYGADPNAAVDGWTALHQIARVRRWNRGFNLP
                     GPEHRDQLSSLELVRKLAAHGADLNARQTGERDGGTTPFLLATKSLDLPYMRTLLELG
                     ADPKLAADDGTTAVMLAAGTGQGQGSLGSAPGGIEEALAALALTLELGAGTVNDVNMR
                     NETPLHGAMYRGGATALLDFLVERGAKLEGVVNSRGWTPLRIADGVALDGV"
     gene            complement(1322..2671)
                     /locus_tag="VM692_05590"
     CDS             complement(1322..2671)
                     /locus_tag="VM692_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ51672.1"
                     /translation="MIITKQALPRRTFLKGAGAVIGLPLLDAMVPAFAANAPTAKRLG
                     FVYLPNGVAKNFTGIDYWTPKQVGKLTELSTILTPLTPYRDRLHVVSGLAATVAEANS
                     EDGATGDHTKATASWLTGVRCKRTEGADIECGTSADQIAAGVLGKDTVLPSLELSIDL
                     SVLAGMCDSAYSCVYLNTLSWTTPKTPIPSENNPRVVFERLFGNGGTNEQRTTQARQD
                     RSILDFVSADFARLTRQVGPTDRVQLDEYLTSVREVERRIQALEKLGDDAGLPALERP
                     TGIPEIFGDHVKLMYELQWLAFQADLTRVVTFMLGRELNFRTYPEVGLNQGHHTMSHH
                     QEKPENIERYAKLNTYQTDLFAWFLNKLASTPEGDGTLLDQSLFMYGAALSNPNLHAH
                     FDLPLAVVGGPAGSGGRHTTVPSNTPMSNLVVTLLDRVGVSVPSLGDSTGRVELGNA"
     gene            complement(2668..5172)
                     /locus_tag="VM692_05595"
     CDS             complement(2668..5172)
                     /locus_tag="VM692_05595"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ51673.1"
                     /translation="MARPSAQHAARPRPSTVGMPAASLLSISALVLAACGPSPEQLAS
                     QQRATIARYCFECHDDAGREADLSLESLDFNHVGANAAVWEHVVRKLQAGMMPPHDGG
                     PRPTSEETAAVLTWLEGALDSAAAAAPDPGRTVPFHRLNRSEYRNAVRDLLAIDVDVA
                     SMLPGDDASYGFDNIGGVLKLSPTLLERYLAAADKISRLAVGTASPFVNYDSYRIPDD
                     RSQEQRLPGMPFGTRGGISIEYMFPQDGDYEISAALARDLNEGMPNYTEPQVLEVSID
                     RKRVATFTLDAATLLPPGPNAEPDDFGGRLTGRVAREEQQARNRADEDWVVRVPVTAG
                     KREVAITFLEKAASVPTGKREPFLRPFPRGLNIPEGRYGSYLRRVEISGPYDATGPGQ
                     TASRERIFTCRPPTEQSAATPESEGCAKSVLASLARRAYRRPVGDADVEPLLDFYRAA
                     HADAGNFDGGIQVALKALLVSPEFLFRIQRDPADAAPGSLYRISDVELASRLSFFLWS
                     SIPDDELLGAAERGVLGKPAELERQVRRMIADPRADAFVSNFAGQWLYLRNLEAVIPV
                     QSIFPNFDDTLREGLRRETELFFASVLREDRSVLELLDADYTFVNERVARHYGIPNVK
                     GSHFRRVPLPADSPRRGLLGQGAILAVTSRADRTSPVVRGQWILENLLGAEPPPPPAN
                     VPPLDATNVTNGQGQMLSLRDRLQAHRANPSCASCHLAMDSLGFALEGFDAVGRYRAI
                     DELGDPIDSSGVLPDGSQFAGFEEFRAALKSSELFRLVLAEKLMVYALGRGIEPYDMP
                     TVRAIVRDAANEDHRFSQFILGVVNSTAFQMRRTGS"
     gene            5410..6405
                     /locus_tag="VM692_05600"
     CDS             5410..6405
                     /locus_tag="VM692_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HUQ51674.1"
                     /translation="MVKPTALFVLGDSLSDVGNAAGAADFLLNLVLRPPTVGLCNLSD
                     VLVLPRDCSNLFYRQARVSNGPVAVELLSAHLGLPALEPSLHLVPQRPVAGTNYAVAS
                     AKARGQNDHDLSRQVDWLLLDHAPLPTDAVYVVMIGGNDAIDALQAHVAGAPDGGRAI
                     VTAAVRAIGDNVERLLDFGARRIVVANVPDLATLPAVHAAASASRDAAPTLAAASAIS
                     AAFALELDARLGAIEASGQWLAPTPPAIARFDLQAALEAARQTIAAGGGNAIDACFDS
                     ATYRASSTAERVFHAGCAPATSDGVPRFATFAFWDGIHPTAAAHAAIGAALGALF"
BASE COUNT          991 a         2222 c         2172 g         1057 t
ORIGIN      
        1 gacgccgtcg agcgcgacgc cgtcggcgat ccgcagcggc gtccagccgc ggctgttgac
       61 gacgccctcg agcttcgcgc cgcgctccac gaggaagtcg agcaacgcag tcgcgccgcc
      121 acggtacatg gcaccgtgca acggcgtctc gttgcgcatg ttgacgtcgt tgaccgtgcc
      181 ggcgccgagc tccagcgtca gcgcgagcgc cgcaagcgcc tcctctattc cgcccggcgc
      241 cgagcccagc gacccttggc cctggccggt cccagccgcg agcatgacgg ccgtcgtgcc
      301 gtcgtccgct gcgagcttcg ggtcggcgcc gagctcgagc agcgtgcgca tgtacggcaa
      361 gtccaacgat ttcgtcgcga gtaagaacgg cgtcgtgccg ccgtcgcgct cgccggtctg
      421 gcgcgcgttg aggtcggcac cgtgggccgc gagcttgcga acgagctcga ggctcgaaag
      481 ctggtcgcgg tgctccggcc ccggcaagtt gaagccgcgg ttccagcgcc gtacgcgcgc
      541 aatttgatgc agcgccgtcc agccgtcgac ggccgcgttc gggtccgcgc cgtagtcgag
      601 taaggcgctc gcagcttcgt aattctggtt cagcgcggcc agcacgatca tgctcgtgcc
      661 gtcgggcaat gcgccgcttg cgtcggcgcc cgcctcgagc agcgccttca cggccacgac
      721 ggcgtcggcg cgcacggcga aagccaatgc cgtgaactcg ccggtcgcct cgcgcgcgtc
      781 gcggtcggcc cccgccttga tcaatgccgc aatcgcgggg ccgttgttct cggcggcggc
      841 cagcatcagg gcagtttgtc cgccgcgctc ttccgcggca ttgacgtcgg cgccggccgc
      901 aacgaggagc tcgatcgtcg cgacggcgcc ggtacgcgcg gccgtcatca gcaacgtctc
      961 gccctcggca aacgtcgcac gcggatcggc accgccttcg agcagcgcgc gcagcgccgc
     1021 cgcatcgccg ttctcagcgg cgaggtacgc ggggcgaacg ccgtaacggt tcgccgcatc
     1081 cgcggccgcg ccggccgtga gcagtgcccg cacgccctcg aggtcgccgc ggtgcacggc
     1141 ccagtgcagc gccgtggtgc cgtcggcgtc tgcagcattt ggatcgacct cggagcgcaa
     1201 cagaccgcgc agctccatcg tcgtaccgtg cttcgcggca tcgagcaagg agccggcgcc
     1261 ctcgcccgct cgcgcgccga cgccgcagag cagcgacaac gcgaagacca cgacttggcg
     1321 attacgcatt gccgagctcg acgcgtcccg tgctgtcgcc gagcgacggg accgacactc
     1381 cgacgcggtc gagcagcgtc acgaccaagt tcgacatcgg cgtattgctg ggcacggtcg
     1441 tgtgccggcc accgctaccg gccggcccgc cgaccacggc gagcggcaaa tcgaagtggg
     1501 cgtgcaaatt cggattgctg agcgccgcgc cgtacatgaa cagcgactga tcgagtagcg
     1561 tgccgtcgcc ctcaggcgtc gaggcgagct tgttcaggaa ccacgcgaac aaatccgtct
     1621 gatacgtgtt gagcttcgcg tagcgctcga tgttctcggg cttctcctgg tgatgcgaca
     1681 tcgtgtgatg gccttggttg agcccgacct cgggataggt cctgaagttg agctcgcggc
     1741 cgagcatgaa cgtcacgacg cgcgtcagat cggcctggaa cgcaagccat tgcagctcgt
     1801 acatgagctt cacgtgatcg ccgaagatct ccggaatgcc ggtcgggcgc tcgagcgccg
     1861 gcaggccggc atcgtcgccg agcttctcga gcgcttgaat ccgtcgctcg acctcgcgca
     1921 ccgaggtcag gtactcgtcc agctgcacgc gatccgtcgg cccgacctgg cgcgtcaacc
     1981 gcgcaaaatc ggcgctgacg aagtcgagga tgctgcggtc ttgacgcgcc tgcgtcgtgc
     2041 gctgctcgtt cgtgccgcca ttaccgaaca accgctcgaa cacgactcgc ggattgttct
     2101 cgctcgggat gggcgtcttc ggcgtggtcc acgacagcgt gttcaagtac acgcagctat
     2161 aggcgctgtc gcacatgccg gccagcacgc tgagatcgat cgacagctcg agcgacggca
     2221 gcacggtgtc tttgccgagg acgccggcgg caatctggtc ggccgaggtg ccgcattcga
     2281 tatcggcgcc ttcggtacgc ttacagcgca cgccggtgag ccacgatgcc gtcgccttcg
     2341 tgtgatcgcc cgtcgcgccg tcctcggaat tcgcctccgc caccgtcgcc gcgagaccgc
     2401 tgaccacgtg cagccggtcg cgatacggag tcagcggcgt caatatcgtc gagagctcgg
     2461 tcagcttgcc gacttgtttc ggcgtccagt agtcgatgcc tgtgaaattt ttcgccacgc
     2521 cgttcgggag gtagacgaac ccaagccgct tcgccgtcgg cgcgttggcc gcgaacgcgg
     2581 gcaccatcgc gtcgagcagc ggcagtccaa tcacggcgcc cgcgcctttc aggaacgtcc
     2641 ggcgcggtaa cgcttgcttc gtaatgatca cgatccggtc ctcctcatct ggaacgccgt
     2701 gctgttcacg acgcccagga tgaactgcga gaagcgatgg tcctcgttcg ccgcgtcgcg
     2761 cacgatcgcg cgcacagtcg gcatgtcgta cggctcgatg ccgcggccga gcgcgtagac
     2821 catgagcttc tcggcgagca cgagccgaaa gagctccgac gatttgagcg cggcgcggaa
     2881 ctcttcgaag ccggcgaatt ggctgccgtc gggcaagacg cccgaagagt cgatcggatc
     2941 gccgagctcg tcgatcgcgc gatagcggcc gacggcgtcg aacccctcga gcgcgaagcc
     3001 gagcgaatcc atcgcgaggt ggcacgacgc gcagctcggg ttcgcacggt gcgcctgcag
     3061 gcgatcgcgc agcgatagca tctggccctg accgttcgtc acgttcgtcg cgtcgagcgg
     3121 cggcacgttc gcgggcggcg gcggcggttc cgcgccgagc aggttctcga ggatccactg
     3181 accgcgcacg accggcgacg tgcgatccgc gcgcgaggtc accgcgagga tggcgccttg
     3241 gccgagcagg ccgcggcgcg ggctgtcggc cggcaacggc acgcggcgga agtgactgcc
     3301 tttgacgttc ggaatgccgt agtgccgcgc gacacgctcg ttgacgaacg tatagtccgc
     3361 gtcgagcaac tcgagcacgc tgcggtcctc gcgtagcacg ctcgcgaaga acagctccgt
     3421 ctcgcggcgc aagccttcac gcagcgtgtc gtcgaagttc gggaaaatgc tctgcacggg
     3481 tatcacggct tcgagattgc gcagatacag ccactggccc gcgaagttgc tgacgaacgc
     3541 atccgctcgg gggtccgcga tcatgcgtcg aacctgacgc tcgagctccg cgggcttgcc
     3601 gagcacgccg cgctcggcgg caccgagcaa ctcgtcgtcg gggatgctgc tccataagaa
     3661 gaaggagagc cgcgaggcga gctcgacgtc gctgatgcga tacagcgagc ccggcgccgc
     3721 gtccgccgga tcgcgctgga tgcggaacag gaactcgggg ctgacgagca aggctttcaa
     3781 cgcgacctgg atgccgccgt cgaagttgcc tgcgtcggcg tgcgcggcgc gatagaagtc
     3841 gagcaacggc tcgacatcgg catcgccgac gggccggcga taggcgcgcc gggcgagcga
     3901 agcgagcacg ctcttcgcgc acccttccga ctccggcgtc gccgccgact gctcggtagg
     3961 cggacggcac gtgaagatcc gctcgcggct cgccgtctgg cccgggcccg tggcgtcata
     4021 cggcccgctg atctcgacgc gccgcagata ggacccgtag cggccttctg ggatgttgag
     4081 gccgcgtgga aacggtcgta ggaacggctc acgcttgccg gtcggcaccg acgccgcttt
     4141 ctcgaggaac gtgatcgcga cctcgcgctt gccggccgta acaggcacac gcacgaccca
     4201 atcctcgtcg gcacgattgc gtgcctgctg ctcctcgcga gcgacgcgtc ccgtcagtcg
     4261 gccgccgaaa tcgtcgggct cggcgttcgg ccccggcggc aacaacgtgg cggcgtcgag
     4321 cgtgaacgtc gcgacgcgct tgcgatcgat gctgacctcg agcacttgcg gctcggtgta
     4381 gttcggcatg ccctcgttca agtcgcgtgc gagcgccgcc gagatctcgt agtcgccgtc
     4441 ttgcggaaac atgtattcga tgctgatgcc gccgcgcgtg ccgaacggca tcccgggtaa
     4501 gcgctgctct tgcgagcgat cgtctggaat gcggtacgag tcgtagttga cgaacggcga
     4561 cgccgtaccg acggcgaggc ggctgatctt gtcggccgcg gcgagatagc gctcgagcag
     4621 cgtcggcgac agcttcaaga cgccgccgat gttatcgaag ccgtagctcg cgtcgtcgcc
     4681 cggcaacatg cttgcgacgt cgacgtcgat ggccagcaga tcgcgcaccg cgttgcggta
     4741 ctcgctacgg ttcaagcggt ggaacggcac cgtgcggccc ggatccggcg cggctgcagc
     4801 tgcgctgtcg agcgcgccct cgagccacgt caacacggcg gccgtctctt ccgaggtcgg
     4861 cctcgggccg ccgtcgtgcg gcggcatcat gccggcctgc agcttgcgga cgacgtgctc
     4921 ccagaccgcg gcgttggcgc cgacgtgatt gaagtcgagc gactcgagcg atagatcggc
     4981 ttcgcggccg gcatcgtcgt ggcactcgaa gcagtagcgc gcgatcgttg cccgctgctg
     5041 cgaggccagc tgttccggcg acggcccgca cgctgcgagc accagggccg agatcgacag
     5101 caacgacgcc gccggcatgc ccacagtcga cggccgtggc cgcgccgcgt gctgtgccga
     5161 tggtcttgcc atgctttccc ccgactaaac acgacgaacg taaccggagg ttataaccgg
     5221 gtcaacgacg cgccgaccgg ctgaacgtca gcttctgtta tggtggccac catgcagagt
     5281 gtcaggacga ctgggctgct cttggttttg ctgttcgctg gatgcgggca aggcggcaac
     5341 gcagagccgc ttgcggcgcc gtccgcgcct gcgttcgcgc ccgtcgacac agcgttatcc
     5401 accgcgccgt tggtcaagcc gacggcgctg ttcgtgctcg gcgatagcct ttccgacgtc
     5461 ggcaacgccg ccggcgccgc cgacttcttg ctaaatcttg tgcttcgccc gccgaccgtc
     5521 ggtctctgta acttgagcga cgtgctcgtt ttgccgcgcg actgctccaa cctcttctat
     5581 cgtcaagcgc gcgtcagcaa cggccccgta gccgtcgagc tcctgtccgc acacctcgga
     5641 ctgccggcgc tcgagccgtc gctgcatctc gtgccgcagc ggccggtcgc gggaacgaac
     5701 tatgccgtcg cgagcgccaa agcccgcggc cagaacgacc acgatctctc gcgtcaggtc
     5761 gattggctgc tgctcgatca tgcgccgctg cccacggacg ccgtctacgt cgtgatgatc
     5821 ggcggcaacg acgcgatcga tgcgctgcaa gcacacgttg ccggcgcgcc ggacggcggc
     5881 cgcgcgatcg tcacggcggc cgtgcgcgcg atcggcgaca acgtcgaacg actgctcgac
     5941 ttcggagcgc gccgcatcgt ggtcgcgaac gtgcccgacc tcgcgacgct gcctgccgtc
     6001 cacgccgccg cgtcggctag ccgtgacgca gcccccaccc tcgcggccgc gagtgcgatt
     6061 tccgccgcgt tcgcgcttga gctcgacgcc agactcggtg cgatcgaggc tagtggccag
     6121 tggctggccc cgacgccgcc ggcgattgcg aggttcgact tacaggcggc actcgaggcc
     6181 gcgcgacaaa cgatcgcggc cggcggcggc aatgcgatcg acgcgtgctt cgatagcgcg
     6241 acctaccgag cgtcgtcgac ggcggaacgg gtctttcatg ccggctgcgc gccggccact
     6301 tccgacggtg tgcctcgctt cgcgacgttc gcgttctggg acggcattca cccgacggcg
     6361 gccgcgcatg ccgcgatcgg cgcggcactg ggcgcgctct tttgacgccg attcaggttc
     6421 gattcaggtc gcgagcccta gg
//
LOCUS       DATLEE010000269         2166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1796716, whole genome shotgun sequence.
ACCESSION   DATLEE010000269 DATLEE010000000
VERSION     DATLEE010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796716"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VM692_05605"
     CDS             complement(<1..201)
                     /locus_tag="VM692_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUQ51675.1"
                     /translation="MPLALHSTRHACLGGHVAALGVERQVATLRGGDVLGELSLMTGE
                     SRAATCVALRDTQAHEISHALFE"
     gene            237..1394
                     /locus_tag="VM692_05610"
     CDS             237..1394
                     /locus_tag="VM692_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUQ51676.1"
                     /translation="MIAYVTDVEGRWDKLTTFVEGNPHVAIVDGRLQLADGVTFVFGG
                     DAIDRGSADRRFVALLLAAKRTYGDRVVLLAGNRDINKLRLVYELGGKPHRRAPSGLA
                     RADLLRWTLANTMGAGTAFEHRLTGLRDAGLPATDDTVVASYLDDLAPGGDMRAYLDA
                     CQLGFRANDTLFLHGGVTAENFLRTPGAGPAADVDDWLARLARFYRDEMETFARGTRP
                     DALIAYQSQGPDKLNQGSVVYARPTDEHGNPQLPPADIISRLLADGIRRVVVGHTPSG
                     DCPAILRDGDFELVLADNSYGRIERGSQVFVDAALHVAARTRLDDGTEHAVRYASEAG
                     PPLGLRVEGTGQLVKARLATGDYLLFRGLPEYKVMQVAASAETLGPLITAR"
     gene            complement(1381..>2166)
                     /locus_tag="VM692_05615"
     CDS             complement(1381..>2166)
                     /locus_tag="VM692_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51677.1"
                     /translation="DEARHHLSQFPASCSARERREQAFGSLYEVGRALGMSKDEVDAT
                     YRKLEDYVLGELDYTKSKTCCWNSSTSAMFDIIMDKARKDEAAADAANTCVAPIVFEN
                     RADGYALWAQHAASLGRAADWRAWSEDEACPQRGVAQDTEAPLDATPYCELEIDGNVC
                     TDPREPNNSRTAAVAASGTVGELRICAADEDWFTIATGGTVKITFHHADGDLDLEAYD
                     ATGGRAASSAGTSDTEQVTVPAGGSVRVFGFSGAKGAYTLTAP"
BASE COUNT          314 a          760 c          740 g          352 t
ORIGIN      
        1 ttcgaacagg gcgtgcgaga tctcgtgggc ctgggtgtca cggagcgcga cgcaggtagc
       61 cgcgcggctc tcgccggtca tcagcgacag ctcgccgagg acgtcaccgc cacgcagcgt
      121 cgcgacctgg cgctcgacgc ccagcgcggc gacatgaccg ccgaggcagg cgtgtcgcgt
      181 cgaatggagc gcaagcggca actgttgcga cggatccagt cgtactttcg gctcggatga
      241 tcgcgtacgt cacagacgtc gagggtcgct gggacaagct gacgacgttt gtcgagggca
      301 acccgcacgt cgcgatcgtc gatggccgcc tgcagctcgc cgacggcgtg acgttcgtgt
      361 tcggcggcga tgcgatcgat cgcggctcgg cggaccgccg cttcgtcgcg ctgctgctag
      421 ccgccaagcg gacgtacggc gaccgcgtcg tgttgctcgc cggcaatcgc gacatcaaca
      481 agctgcggct ggtctacgag ctcggcggga agccgcatcg gcgcgccccg tcgggcctcg
      541 cgcgcgccga cttgttgcgc tggacgcttg ccaacacgat gggcgccggc acagcgttcg
      601 agcaccggct gaccgggctg cgcgacgcgg ggttgcccgc gaccgacgac accgtggtcg
      661 cgtcgtacct cgacgatctc gcgcccggcg gcgatatgcg cgcgtacctc gatgcgtgcc
      721 agctcggctt tcgcgccaac gacacgctgt tccttcacgg cggcgtcacc gccgagaact
      781 ttttgcgaac gcccggtgcc ggccccgcgg ccgacgtcga cgactggctc gcccggctcg
      841 cacggttcta tcgcgacgag atggagacct tcgcgcgcgg caccagaccc gatgcgctga
      901 tcgcgtacca gtcgcaaggc cctgacaagc tcaaccaggg cagcgtcgtc tacgcgcgtc
      961 cgaccgacga gcacggcaac ccgcagctgc cgcccgccga catcatctcg cgcctactcg
     1021 ccgacggcat ccggcgcgtc gtcgtcggtc acacgccgag cggcgattgc ccggccatcc
     1081 tgcgcgacgg cgacttcgag ctcgtgctcg ccgataactc gtacggccgc atcgagcgcg
     1141 gctcgcaggt gttcgtcgat gcggcgctgc acgttgccgc caggacgcgg ctggacgacg
     1201 gcacggagca cgcggttcgc tatgcgagcg aagccgggcc accgctcggt ctgcgcgtcg
     1261 aagggaccgg ccagctcgtc aaggcgcggc tcgctaccgg cgactatctg ttgttccgtg
     1321 gcctgcccga gtacaaggtc atgcaggtcg cggcatcggc cgaaacgctc ggtccgctga
     1381 tcacggcgcg gtgagcgtgt acgcgccctt ggcgcccgag aacccgaaca cgcggacgct
     1441 gccgccggca gggaccgtca cctgctccgt gtcggaagtt cccgcgctgc tggccgcacg
     1501 accgccggtg gcatcgtagg cctcgagatc gagatcgcca tcggcgtgat ggaacgtgat
     1561 cttgacggtg ccacctgtcg cgatcgtgaa ccagtcttca tcggcagcgc agatccgtag
     1621 ctcgccgacc gtgccgctcg cggcgactgc ggcggtgcgc gagttgttcg gctccctggg
     1681 atccgtgcac acgttgccgt cgatctcgag ctcgcagtac ggcgtcgcat cgagtggcgc
     1741 ctcggtatcc tgcgcgacgc cacgttgcgg gcaggcctcg tcttcgctcc acgcacgcca
     1801 gtcggccgcg cgaccgagcg atgcggcgtg ctgcgcccac agcgcgtagc cgtcggcgcg
     1861 attctcgaac acgatcggcg cgacgcaggt attcgcggcg tcggcggcgg cctcgtcctt
     1921 gcgggccttg tccatgatga tgtcgaacat cgccgacgtg ctcgagttcc agcagcacgt
     1981 cttgctcttg gtgtagtcga gctcgccgag cacgtagtcc tcgagcttgc gataggtcgc
     2041 gtcgacctcg tccttgctca tgccgagcgc gcggccgacc tcgtagagcg agccgaaggc
     2101 ctgctcgcgt cgctcgcgcg ccgagcacga cgccgggaac tgcgaaaggt ggtggcgcgc
     2161 ttcgtc
//
LOCUS       DATLEE010000270         2605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_306712, whole genome shotgun sequence.
ACCESSION   DATLEE010000270 DATLEE010000000
VERSION     DATLEE010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306712"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VM692_05620"
     CDS             complement(<1..228)
                     /locus_tag="VM692_05620"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51678.1"
                     /translation="MSDKTAVWAALAALGCAASVQAHHSGYMYATTPTWMKGTVTRFE
                     RINPHSVVTLEDRSEGGQVRRWVIEGPPATEL"
     gene            complement(283..1551)
                     /locus_tag="VM692_05625"
     CDS             complement(283..1551)
                     /locus_tag="VM692_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ51679.1"
                     /translation="MTTVSRDLPNKPHLDVAKRLARELLAQWRSGVRDAHDRIRRRHP
                     KFERADDAAVAAGPFRLSDAQLVVAREYGMAHWAELKQRIAANTAAQALATAIRANDV
                     AAAVQLVAHEPQLLHVPLVSGNWGPPMSHAANFGHLEIIKAMAALGAKDFQHALGRAV
                     LQGRTECARWLLEHGDATLEPGLAMGACEGLNVEGMRFLAQIGSPLTNDAGDALGPLA
                     MTLETYSRNPKSKHEILEIFARSGYDLPDTPIMAFHRGSRIELERHLRRDPKLLERRF
                     TLAEIYPAECGCGGNGPGMHWTPIAGTTLLHLAFDFHELDIAAWLLHSGADVDARADV
                     DADGFGGHTPLFNALVCGPWHDDVPTRVLLGHGASTDARASLRKFLDWIEEPRWHVAR
                     DVTAAEWARGFPDRSWSNGKALRLLDGEGS"
     gene            complement(1705..>2605)
                     /locus_tag="VM692_05630"
     CDS             complement(1705..>2605)
                     /locus_tag="VM692_05630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ51680.1"
                     /translation="CAESWLNDRADRDSRGTLLASYMVVIYVGIGGGQFLLNAADPRG
                     HELFVLIGVLISVAVVPIALTAQRAPEFALPRRVTFRELLAISPLGTVGAFFSGFATA
                     TFFAVGPVYAVRSGLDTGEVATFMALGALPAIVLQLPLGRLSDRFDRRSVLMAMAISA
                     AVAAMLVLQARGGTPPFFAAVALYGGLSSTMYALCVSHVNDHLHPEQMVAASATVLLL
                     NGTGSALGPLLVSAAMQLTQPGAFFVSAVVMHGAFALYAFWRKRKVEPIPDAAKTRFA
                     GAPPQVAPTGRLAALEQAPKRVD"
BASE COUNT          439 a          892 c          863 g          411 t
ORIGIN      
        1 gagctcggtt gccggcgggc cttcgataac ccaacggcga acctggccgc cctcgctcct
       61 atcctcgagc gtgacgacgc tatgcggatt gatgcgctcg aaacgcgtga ccgttccttt
      121 catccacgtg ggtgtcgtcg cgtacatgta gccggaatga tgtgcttgca ccgaagctgc
      181 acagccgagc gccgcgaggg ctgcccacac cgctgtcttg tcgctcattg gcccgccaat
      241 ctctacgttc gtagatatat agtatctaca gacgtagaga tatcaactcc cctccccgtc
      301 caagagccgc agcgccttgc cgttcgacca gctccggtcg ggaaacccgc gcgcccattc
      361 ggccgccgtc acgtcgcgcg ctacgtgcca tcgcggttct tcgatccagt cgaggaactt
      421 gcgcaggctc gcgcgcgcat cggtcgaggc gccgtggccg agcagcacgc gcgtcggcac
      481 gtcgtcgtgc caaggtccgc agaccaacgc gttgaacagc ggcgtgtgac cgccgaagcc
      541 gtcggcgtcg acgtccgcgc gagcgtcgac gtctgctccg ctgtgcagca gccacgccgc
      601 gatgtcgagc tcgtggaaat cgaacgcgag gtgcagcagc gtcgtgccgg cgatcggcgt
      661 ccaatgcatg ccgggtccgt tcccgccgca gccgcactcg gcggggtaaa tctccgccag
      721 cgtaaaccgg cgctcgagca acttgggatc gcggcgcagg tgccgctcga gctcgatgcg
      781 gctgccgcga tgaaacgcca tgatcggcgt gtcgggcagg tcgtagccgc tgcgcgcgaa
      841 gatctcgagg atctcgtgct tgctctttgg gttgcggcta taggtctcga gcgtcatcgc
      901 aagcgggccg agcgcgtcgc cggcgtcatt cgtcagcggc gagccgatct gtgcgagaaa
      961 ccgcatgccc tcgacgttca ggccttcgca ggcacccatc gcaagcccag gctcgagcgt
     1021 cgcgtcgccg tgctcgagaa gccaacgcgc gcattcggtt ctgccctgca gcaccgcgcg
     1081 accgagcgcg tgctgaaagt ctttcgcacc caacgccgcc atcgctttta tgatctcgag
     1141 gtggccgaag ttggccgcgt ggctcatcgg cggcccccag ttgccgctga ccaggggcac
     1201 gtgcaacagc tgcggctcgt gcgcgacgag ctgcaccgcc gccgcgacgt cgttggcacg
     1261 aatcgccgtc gcgagtgcct gtgccgccgt gttcgcggcg atacgctgct tgagctcggc
     1321 ccagtgcgcc atgccgtatt cgcgagcgac gacgagctgc gcgtcgctca agcgaaacgg
     1381 gcccgccgca accgccgcgt cgtcggcacg ctcgaacttt ggatgtctgc gccggatgcg
     1441 gtcgtgcgcg tcgcgcacgc cgctacgcca ctgcgcgagt agctcgcgcg ccagccgctt
     1501 cgcaacgtcg agatggggct tgttgggtaa atctcgggat acggtagtca caacctttcc
     1561 tcctatgggc gcccatggtc cgcacacctc gggcaggaag tcaggttgag tcgtcgcaac
     1621 acatcaccgg tgggcttggc ccatttccgc ggacagggtg cttccggtaa gcgcccatag
     1681 catggctcga ctgcgggtcg cggatcaatc gactcgcttc ggcgcttgct cgagcgcagc
     1741 gagccgcccc gtcggcgcga cttgcggcgg cgcgcccgcg aaccgcgttt tcgcggcgtc
     1801 ggggatcggc tcgaccttgc gcttgcgcca gaaggcatag agtgcaaacg cgccgtgcat
     1861 gactacggcg ctgacgaaaa acgcgcccgg ctgggtcagc tgcatcgccg cggacacgag
     1921 caacggcccg agcgcggagc cggtgccgtt caacagcagc acggttgcgc tcgccgcgac
     1981 catctgctcg gggtgcaagt ggtcgttgac gtgcgacacg cagagcgcat acatcgtcga
     2041 cgacaaaccg ccgtacagcg ccacggcggc aaagaacggc ggcgtgccgc cgcgagcttg
     2101 caggacgagc atggcggcga ccgccgcgga gatggccatc gccatcagga cgctgcggcg
     2161 atcgaagcga tccgacaacc ggccgagcgg cagctgcagc acgattgccg gcagagcgcc
     2221 gagcgccatg aacgtcgcaa cctcgccggt gtcgaggccg ctgcgcacgg catagaccgg
     2281 cccgacggcg aagaacgtcg ccgtcgcgaa tcccgagaag aatgcgccga cggtcccgag
     2341 cggcgagatc gcgagcaact cgcgaaacgt cacgcgccgc ggcaacgcga actcgggggc
     2401 tcgctgcgcc gtcaacgcga tcggcacgac cgcgaccgag atcaatacgc cgatcaggac
     2461 gaacagctcg tggccgcgcg ggtccgcggc gttcagcagg aactggccgc cgccgatgcc
     2521 gacgtagatc acgaccatgt agctcgcgag caaggtcccg cgcgagtcgc gatcggcgcg
     2581 atcgttgagc cagctctccg cgcag
//
LOCUS       DATLEE010000271         6701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2542935, whole genome shotgun sequence.
ACCESSION   DATLEE010000271 DATLEE010000000
VERSION     DATLEE010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542935"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1292)
                     /locus_tag="VM692_05635"
     CDS             complement(24..1292)
                     /locus_tag="VM692_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUQ51681.1"
                     /translation="MSTIAWVLISCVATALVCGLAVMLTRARGTPYLELKPDEYPEIK
                     DALPLIAGLTESSVHEGNSAAVFQNGAIFPAMLKDIAAAQHSVHLETFVWTAGKLETQ
                     FVDALGERARAGINVRVLLDCLGASKASEAELERLRQSGAKLSTYCKPQWWNMGRFNH
                     RTHRKLLVVDGRIAYTFGHGVADQWLGNGTDPEHWRDTGVRFEGPVVHGLQSVFAQNW
                     VEETHCLPAGDECFPVLEPAGDISAHVVSSASADAVSSVARLYTVAIAMARREVLIQN
                     PYFAPNDGVVELFAMMVRRGVNVHLMLPGKHTDSPFVRRAGCNLYTALLEAGVKLYEF
                     EPTLLHQKIVVVDEIWSHVGSTNFDARSLALNEEVGVGILDAGIAADLKAAFKQDLKR
                     CRELTLDEWQRRPLFDRAYERFAYLLHEQL"
     gene            1436..1705
                     /locus_tag="VM692_05640"
     CDS             1436..1705
                     /locus_tag="VM692_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HUQ51682.1"
                     /translation="MTFIAWIVVGGLLGWVASMIMGTNDQQGKFLNIIVGIVGAFLGG
                     LLIAPLLGTGTINDNDFSVGSLLTSLIGAVVLLAIVGFFRKRRRA"
     gene            complement(1774..2781)
                     /locus_tag="VM692_05645"
     CDS             complement(1774..2781)
                     /locus_tag="VM692_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUQ51683.1"
                     /translation="MTTGQGEKRPTLASWAALLTILAVAGCAREEEPAPAPTPPPAAA
                     PRPAPAATAAATVRDSTDPLQHGRYLVETVAGCGNCHTPRLPDGTPDPTKNLAGAFVI
                     EEPVFKAYAQNITPDMETGIGSWTEDQIVDAIRNARRPDGTFMGPPMSFGWYKRMSDS
                     DAHAIAKYIKTVPAVRNAVPESTYQMGPDGPRTGNIYGPIVTSVPDMPKTDIVKYGEY
                     LAGPVGHCMDCHTTYVMGQIDLTQLGRGGNVYSKPFIYDWAAVSANITSHPEAGLGAW
                     TDAEIKRAITEGVSRNGRQLLPFMPYWLYKKMEPSDLDAIVAYLRSLPPLGPAATARP
                     E"
     gene            3056..4360
                     /locus_tag="VM692_05650"
     CDS             3056..4360
                     /locus_tag="VM692_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51684.1"
                     /translation="MAVEAGLLREQAAKIVQSGALGRSRSYARLLEFLVECGVSGRTP
                     KELEIAMEVFGRGADFDPSQDSMVRVYAHNLRQKLEHFYATAGRTEPHQLQLARGEYR
                     VSLAAGEVAVEAPEVAPVTPPPAPVERAATFTGWRLAVAAAALVAVGTALGVGIMVEQ
                     APTAPAAAAVAKSPIWADMLDDDLPILIVVGDYYIYGELDEHGDVTRLVRDFSVGSSR
                     ELDELMKNDASLMARYLDLDLTYLPTGAASALLDVLRVLYTSNKPLRVISMSEMNEAD
                     LKSSHVIYIGYISALSKLEDFVFASSSLAVGYTYDELRNLDTGELYTSEAGMPEINRN
                     YRDYAFISTFPGPGGNQLMIVSGTRDAGLMQAAHAVADPMFVQSMEKVRPDSGDSRPP
                     SFEMLYEVTGYGRTNLDAMLVHTAELNYQEIWGGIFRQAPPN"
     gene            complement(4353..5294)
                     /locus_tag="VM692_05655"
     CDS             complement(4353..5294)
                     /locus_tag="VM692_05655"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HUQ51685.1"
                     /translation="MTAAAVESQTADAVLMVRPAHFAGNDETAASNFFQHAVAGIADA
                     AERAQREFDALALVLADAGVRVHQFAGQRGAFLPDEIFPNNWLSLHADGTAVLYPLLA
                     ANRRRERRADILSSLVDSCGYRLERVVDLTSLEARNEYLEGTGSLVLDRARRVAYACR
                     SPRTHENALAEFARALRYEIVSFAAVDAAGRAIYHTNVLLSLGTRFAALCTSAIPDAR
                     ERHGVIEQLESSGRVVVDLRAQELASFAGNLLELRGRGGSVIALSAAALGSLAAPTRR
                     TLERYGQVVAADIATIERLGGGSVRCMLAEVALPRIS"
     gene            complement(5302..6141)
                     /locus_tag="VM692_05660"
     CDS             complement(5302..6141)
                     /locus_tag="VM692_05660"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HUQ51686.1"
                     /translation="MPFFPQTDYQCGPAALATLLASSGAAVDADDLVREVYVEGLHGS
                     LQPELLGATRRHGFVPYVIAPDASALLAELSARRPVLVLQNLGVARVPVWHYAVVVGV
                     AEGGVILRSGTQERRVERGTGFLRSWQRGANWAFVALEPGEMPATATAGAYIRALAGA
                     EPLLDAVAAERGYRAALARWPSDELVLFAAAGQRYAAGDLAGATTLYRQLLAAAPQHA
                     AARNNLANVLGARGCHAEALAEARSALAAVAPTDELGAAVRDTVAELERAAAAGAPQA
                     SCQ"
     gene            complement(6230..6610)
                     /locus_tag="VM692_05665"
     CDS             complement(6230..6610)
                     /locus_tag="VM692_05665"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HUQ51687.1"
                     /translation="MPHRHRTPLVIVLTASMFAASLQSAAYGGVIGTQQYLTAIDREA
                     TRQRIDSVLAREEVRDRLEQYGVDPAALDERIAALTDQELQQLATDLDNMPAGGSLLA
                     VIGVAFIVLLILELVGVIDIFNKI"
BASE COUNT         1101 a         2206 c         2273 g         1121 t
ORIGIN      
        1 agtctcggcg ccgtcgcgct cgattacagc tgctcgtgca acaggtacgc gaatcgctcg
       61 taggcgcggt cgaacaacgg tcggcgctgc cattcgtcga gcgtgagctc gcggcagcgc
      121 ttcaaatcct gcttgaacgc ggccttgagg tcggcggcaa tgccggcatc gagtatgccc
      181 acgccgacct cttcgttgag ggccagcgaa cgcgcgtcga aattcgtcga gccgacatgc
      241 gaccaaatct cgtcgacgac gacgatcttt tggtgcagta gcgtcggctc gaactcgtaa
      301 agcttcacgc cggcctcgag caacgcggta tagagattgc agccggcgcg gcgcacgaac
      361 gggctgtccg tgtgcttgcc gggcagcatc aggtgcacgt tgacgccgcg gcgcaccatc
      421 atggcaaaca gctcgacgac gccgtcgttc ggcgcgaagt acgggttttg gatcagcacc
      481 tcgcgccgcg ccatcgcaat cgcgacggta tagagccgcg cgacggatga aacggcgtcc
      541 gccgaggcgc tgctgacgac gtgcgctgag atgtctccgg ccggctcgag cacggggaag
      601 cactcgtcgc cggccggtag gcaatgagtc tcctcgaccc aattctgggc gaacaccgat
      661 tgcaacccgt gcacgacggg cccttcgaag cgcacgccgg tgtcgcgcca atgttcaggg
      721 tcggtcccgt tgccgagcca ttgatccgcg acgccgtggc cgaacgtgta cgcgatccgc
      781 ccgtcgacga cgagcagctt gcggtgcgtt cggtggttga aacggcccat gttccaccac
      841 tgtggcttgc agtacgtcga cagcttggcc ccgctttgtc tcaaccgctc gagctccgct
      901 tcgctggcct tcgacgcgcc gaggcaatcg aggagcacgc gcacgttgat gccggcacgc
      961 gcgcgctcgc cgagcgcatc gacgaattgg gtttcgagct tgcccgccgt ccaaacgaaa
     1021 gtttccagat ggacgctgtg ctgcgcggct gcgatgtctt ttagcatcgc gggaaagatc
     1081 gcgccgttct ggaacacggc cgcgctgttc ccctcgtgaa ccgagctctc cgtgaggccc
     1141 gcgatcagtg gcagcgcgtc tttgatctcg gggtactcgt cgggtttgag ctcgagatac
     1201 ggcgtgccgc gagcacgcgt cagcatcacg gcgagcccac acaccagtgc cgtcgccaca
     1261 caagagatca gcacccatgc aatcgtcgac atagataccc catcgcgtcc ccgcgcatgg
     1321 cacggaggtg caataaatga gccagggctc cgcggtgtgc gcgagggcac gctacttgca
     1381 agacttcagc gttccggcag tccgccggct tgtgaactcg tcaaggagtc gtgccatgac
     1441 ctttattgct tggatagtcg tcggcggtct gctgggttgg gtcgccagca tgatcatggg
     1501 aacgaacgat cagcagggca aattcctcaa catcattgtc ggaatcgtag gggcctttct
     1561 cggcggcttg cttatcgcgc cgctattggg caccggcacg atcaacgaca acgatttcag
     1621 cgtcggctcg ctgctgacgt cgctgatcgg tgccgtcgtg ctgctcgcca tcgtgggatt
     1681 cttccgcaag cgacggcgag cctaggcctc aaaaagattg cggcgcggta cccgaaggca
     1741 ccgcgccgca agctcggact cgagcgtgtc cgactactcc ggccttgcgg tagcggccgg
     1801 gccgagcggc ggcagcgagc gcaggtacgc gacgatcgcg tcgagatcgc tcggctccat
     1861 cttcttatag agccagtacg gcatgaacgg cagcagctgc cggccattgc gactcacgcc
     1921 ttcggtgatc gcgcgcttga tctctgcatc cgtccacgca cccaagccgg cttccggatg
     1981 cgacgtgatg ttcgccgaga cggcggccca gtcgtagatg aacggcttcg agtagacgtt
     2041 gccgccgcgg ccgagctgcg tcaggtcgat ctgtcccatg acgtacgtcg tgtggcaatc
     2101 catgcaatgg ccgaccgggc ccgcgaggta ctcgccgtac ttcacgatgt ccgtcttcgg
     2161 catatcgggc acgctggtga cgatcgggcc gtagatgttg ccggtccgcg gcccgtcggg
     2221 ccccatctga tacgtgctct ccggcaccgc gttgcgaacg gcgggcaccg tcttgatgta
     2281 cttcgcgatc gcgtgcgcgt cgctgtccga catgcgcttg taccacccga acgacatcgg
     2341 cgggcccatg aacgtgccgt caggtctgcg cgcgttgcgg atcgcgtcga cgatttggtc
     2401 ctccgtccac gagccgatgc cggtctccat gtcgggcgtg atgttctgcg cgtacgcctt
     2461 gaagaccggc tcctcgatga cgaaagcacc ggccaagttc ttcgtgggat ccggcgtgcc
     2521 gtcgggcagg cgaggtgtat ggcagttgcc gcagcctgcg accgtctcga cgagatagcg
     2581 accatgctgg agcgggtcgg tgctgtcgcg cacggtcgcg gcggcagtgg cggccggtgc
     2641 gggccgcggc gctgcggccg gcggcggcgt gggagccggc gcgggctctt cctcgcgggc
     2701 acaccccgcg actgcaagaa tcgttagcaa tgcagcccaa ctcgcgaggg tcgggcgttt
     2761 ttccccttgg ccggtcgtca cctgttcttc tcccgttgta atggcgcagg acacggcggc
     2821 aagcatacga aaaaacgggc ggagtccgga agaacttgcg cgcccaaggt tggcgtcgcg
     2881 ctcgccgttc ggagggcgct ggcggcgatg gcgcggagcc cggcacgtgg gcgccgcgcg
     2941 acggattatg gattcttcaa agaaaaccag agcttaagta tggtttatta acggttaagg
     3001 atgggctaac ttgggcgcct tggggcagta aggcgccgct tcgtcagcga gcatgatggc
     3061 agtggaagca gggctcctgc gggagcaagc agcaaagatc gtgcaaagcg gcgcactcgg
     3121 ccgctcgcga tcgtacgcgc ggctgctcga gtttctcgtc gagtgcggcg tcagcggtcg
     3181 aacgccgaag gagctcgaga tcgcgatgga ggtcttcggc cgcggcgcgg acttcgatcc
     3241 gagccaagac tcgatggtgc gcgtctacgc gcacaacctg cggcagaagc tcgagcattt
     3301 ctacgcgaca gcgggccgca ccgaaccgca ccagctacag ctcgcgcgcg gcgaataccg
     3361 agtctcgctc gcggccggcg aggtcgccgt cgaagctccc gaggtcgcgc ccgtcacgcc
     3421 gccgcccgcg cctgtcgagc gcgcggcaac gttcacgggc tggcgtctcg cggtcgccgc
     3481 cgcggcgttg gtcgcggtcg gtactgcgct cggcgtcggt atcatggtcg agcaagcgcc
     3541 tacggcgccg gcggcggccg ctgtcgcgaa gtcgccgatc tgggccgaca tgctcgacga
     3601 cgatttgccg atcctgatcg tcgtcggcga ctactacatt tacggcgagc tcgacgagca
     3661 cggcgacgtg acccgcttgg tgcgcgactt cagcgtcggc tcgagccgcg agctcgacga
     3721 gctcatgaag aacgacgcgt cgttgatggc gcgttacttg gatctcgacc tcacgtattt
     3781 gccgacgggc gctgcgtccg ccttgctcga cgtgctgcgc gtgctctaca cgtcgaacaa
     3841 gccgctccgc gtcatctcga tgtcggagat gaacgaagcg gatctgaagt cgagccacgt
     3901 catctatatc ggctacatca gcgcgctcag caagctcgag gacttcgtgt tcgcatcgtc
     3961 gagcctcgcg gtcggctaca cctacgacga gctgcgcaac ttggataccg gcgagctgta
     4021 cacgagtgaa gccggcatgc cggagatcaa ccgcaactac cgcgattacg ctttcatctc
     4081 gacgttcccg gggccgggcg gcaatcagct catgatcgtc agcggcacac gcgacgccgg
     4141 tctcatgcag gccgcgcacg cggttgccga cccgatgttc gtgcaaagca tggagaaggt
     4201 gcggcccgac tccggcgatt cgcgaccgcc gtcattcgag atgctctacg aggtcacggg
     4261 ctacggccgc acgaatctcg atgcgatgct cgtgcacacg gcggagctca actaccagga
     4321 gatctggggc ggcatcttcc gccaagcgcc gcctaactga ttcgcggcaa cgctacttcg
     4381 gcgagcatgc agcgcacgct gccgccgccg agccgctcga tcgtcgcgat gtcggctgcg
     4441 acgacctgac cgtaccgctc cagcgtgcgc cgcgtaggtg cggccaacga gccgagagcc
     4501 gcagccgaca gcgcgatgac ggagccgccg cggccgcgca gctcgagcag gttgcccgcg
     4561 aagctcgcga gctcctgagc ccgcaaatcc acgacgactc ggccggagct ttcaagttgc
     4621 tctatcacgc catgccgttc gcgcgcgtcg gggatcgcgc tcgtgcagag agcggcgaag
     4681 cgcgtgccga gcgacagcag gacgttcgtg tggtaaatcg cgcggccggc ggcgtcgacg
     4741 gcggcgaacg agacgatctc gtagcgcagc gcacgcgcga actcggccag cgcgttctca
     4801 tgcgtgcgcg gcgagcggca ggcgtacgcg acgcggcgcg cccggtcgag cacgaggctg
     4861 cccgtgcctt cgaggtactc gttgcgagct tcgaggctcg tgagatcgac gactcgctcg
     4921 agtcgatagc cgcacgagtc cacgagcgac gacaggatgt cggcgcggcg ctcacggcgg
     4981 cggttggcgg cgagcagcgg atacaagacg gccgtgccgt cggcatgcag gctcaaccag
     5041 ttgttcggga aaatttcgtc gggcaggaat gcgcctcgct gccccgcgaa ctggtggact
     5101 cgcacgccgg cgtcggccag cacgagggcc agtgcgtcga actcgcgctg cgcgcgctcg
     5161 gccgcgtcgg caatgccggc aaccgcgtgt tggaaaaagt tcgaggccgc cgtctcgtcg
     5221 ttgccggcga agtgcgccgg ccggaccatc agcaccgcgt cggccgtctg gctttcgacc
     5281 gccgctgcgg tcacggctcc ctcattggca gctcgcctgc ggcgcgccgg ccgccgccgc
     5341 gcgttcgagc tccgcgaccg tgtcgcggac ggccgcgccg agctcgtcgg tcggcgcgac
     5401 ggcggccagt gcgctgcgcg cctcggcgag cgcttcggcg tgacagccgc gagcgcccag
     5461 cacgttcgct aggttgttgc gtgcggcggc gtgctgcggc gcggctgcta gcaattggcg
     5521 atagagcgta gtcgcgccgg caaggtcgcc ggccgcgtag cgttgaccgg cggcggcgaa
     5581 cagcacgagc tcgtcgctcg gccaccgcgc caacgcggcc cgatagccac gctcggccgc
     5641 aacggcgtcg agcagcggct ccgctcccgc cagcgctcga atataggcgc ccgctgtggc
     5701 cgtcgcgggc atctcgccgg gctcgagcgc gacgaacgcc cagttcgcgc cgcgctgcca
     5761 tgaacgcagg aatccggtgc cgcgctcgac acgccgctcc tgcgtgcccg agcgcaggat
     5821 gacgcccccc tcggcgacgc cgacgacgac ggcgtagtgc cacaccggca cgcgggccac
     5881 gcccaaattt tgcaggacga gcacgggccg gcgcgcgctg agctcggcca gcagggcgct
     5941 cgcgtcgggc gcgatcacat agggaacgaa gccgtggcgg cgcgtcgcgc cgagcagctc
     6001 cggttgcagg ctgccgtgca ggccttcgac gtacacctcg cgcacgaggt cgtccgcgtc
     6061 gaccgcggcg cccgagctcg cgagcagcgt tgcgagcgcc gcgggcccgc attgatagtc
     6121 cgtctgcggg aagaagggca ccgccgtcag ctcgatggct gccgtcgccg gcagttgcgg
     6181 agcgccggcg cagccggcaa gggcgagcgc ggcggcgagg aggccggccc taaatcttgt
     6241 tgaaaatatc aatgacgccg acgagctcga ggatcagcag cacgatgaac gcgacgccga
     6301 tgacggcgag caggctgccg cccgcgggca tgttgtcgag gtccgtcgcc aactgctgca
     6361 gctcttgatc cgtgagggcc gcgatgcgct cgtcgagcgc ggccgggtcg acgccgtact
     6421 gctcgagccg atcgcggact tcctcgcgag ccagcacgga gtcgatgcgt tgccgcgtgg
     6481 cttcgcggtc gatagccgtc agatactgct gtgtgccgat gactccgccg tacgctgcgc
     6541 tctggagcga cgctgcgaac atcgaagccg ttagcacgat gacgagcggt gtgcgatgac
     6601 gatgtggcat ggcagatctc cttatccctc cgcggcaaac gttagccgag gcccggtagg
     6661 tgcgctagtc ctcaccccac caggcgacga actcttggaa g
//
LOCUS       DATLEE010000272         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6863074, whole genome shotgun sequence.
ACCESSION   DATLEE010000272 DATLEE010000000
VERSION     DATLEE010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6863074"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..539)
                     /locus_tag="VM692_05670"
     CDS             complement(102..539)
                     /locus_tag="VM692_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51688.1"
                     /translation="MNLGRLLLAGLLAGLVLNIGEAVLHGVVLAEPTAAALTALGRSD
                     ASSPAGLTMLVGITFVQGIIGLWLHALLSGRGVTRGRAAVVAGLALWVLSAVYAAIYF
                     GSGFPNVMPAYVIWLPVVWALVEFPLAIFVGALLIPPPKRGLT"
     gene            complement(536..1045)
                     /locus_tag="VM692_05675"
     CDS             complement(536..1045)
                     /locus_tag="VM692_05675"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HUQ51689.1"
                     /translation="MRWGLLLLLGAWSAAGADEVSFVNEGVVDAPVEEVWKIFATSEG
                     YKVLGPALADVDLRIGGTIRSRYGADGTLGDAETIENTILAYEPPTMMATSIAKTPAT
                     FPFKSAWKSTWTVVTLVPVERGKTLLRASSLGFGSDEESVAMRRFFEAGNQSVIASVQ
                     RHFAQRSNP"
     gene            complement(1042..1731)
                     /locus_tag="VM692_05680"
     CDS             complement(1042..1731)
                     /locus_tag="VM692_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUQ51690.1"
                     /translation="MTAQEQEPVAVETRDRLLKTAFQLFHEQGYHATGVATILREAGV
                     NAGSLYHFFSSKDDLLLEVLEFALDYLEPMVMGPAEAAAVDPLERVFVLLNQYREWVG
                     ANQCRMGCPIGNLALEVSDGNPRARELIHRNFENWAGRVEGWLRSAGDELPPGTDLRR
                     LARFVLTVMEGGLMQARAAGHIGPFDDSVAVLRAHIDALRAQAAGTRGARARAAKKIN
                     RVKNKKRSKSR"
     gene            complement(1824..2261)
                     /locus_tag="VM692_05685"
     CDS             complement(1824..2261)
                     /locus_tag="VM692_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51691.1"
                     /translation="MQIRPQSWLYLAAFAVLGSGCASTTSTDEDARSARLPTGVTLLD
                     SDRNECSGSVAIDERSVANARRADLVIQRGQNAVFQIDADDNDDVEIDWTCVGAANTD
                     RNTVECPDETEYVRITRATTGNDFLLECYGDRDNDRDRRVSRR"
     gene            complement(2342..3013)
                     /locus_tag="VM692_05690"
     CDS             complement(2342..3013)
                     /locus_tag="VM692_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51692.1"
                     /translation="MFPLRPLRVIVTLAAVAGFAQAALAGPPLICHPFITDAAAPMLP
                     WLPTRDWHSPDPSYDVARLAADTLKLLSPDARVIDRMENMRRAAIYAERDLGLAESLL
                     TAVVERTKTPQADARAAAMAWFDAGYLLETYRQLDLIYRYEMRPAHGRPATIVPRAQL
                     AVDGYALLRRARAQAPVTELPEIEFAASLMTQQRVASAHRDKAKAGAVPGSLLARNLE
                     MFWTD"
BASE COUNT          566 a         1092 c         1028 g          521 t
ORIGIN      
        1 gtgagccggc tcgtcgatcg gctcatgggc gcgcccggcg gcgcgggcgg cgaacccgtt
       61 ctccacgacc tccgccgcga ctagcgacgc tcgtcgcgac gctaggtcag ccctcgcttc
      121 ggcggcggga tcagcagcgc gccgacgaag atcgcgagcg gaaattcgac gagcgcccaa
      181 acgacgggca gccagatgac gtacgccggc atgacgttcg ggaaacccga gccgaaatag
      241 atcgccgcgt agaccgccga caacacccac agcgccaagc cggcgacgac ggcggctcgc
      301 ccgcgcgtca cgccgcgtcc tgataacagc gcgtgcagcc agaggccgat gatgccctga
      361 acgaacgtga tgccgaccag catcgttagc cccgccggcg agctcgcatc gctgcggccg
      421 agcgccgtca gcgcggccgc cgtcggttcg gcgagcacga cgccgtgcaa aacggcctcg
      481 ccgatgttca atacgaggcc ggccagcaag ccggccagca acagcctgcc gagattcatg
      541 ggttgctccg ttgcgcgaag tggcgctgca cgctcgcgat gaccgattga tttcccgcct
      601 cgaagaaacg gcgcatcgcg accgactcct cgtcgctgcc gaaacccaag ctcgaagcgc
      661 gcagcagcgt cttgccgcgt tcgacgggca cgagagtcac gaccgtccac gtgctcttcc
      721 acgcgctctt gaacgggaac gtcgcgggtg ttttggcgat gctcgtggcc atcatcgtcg
      781 gcggctcgta cgcgagaatc gtgttctcga tcgtctcggc gtcgcccagc gtgccgtcgg
      841 cgccgtagcg cgagcggatc gtgccgccga tgcgcagatc gacgtcggcg agcgccgggc
      901 ccagcacctt gtacccttcg gacgttgcga aaatcttcca gacttcctcg acgggcgcgt
      961 cgacgacgcc ttcgttgacg aacgacactt cgtcggcgcc ggcggccgac cacgcaccga
     1021 gcaataacag caaaccccat ctcatcgcga cttactccgt ttcttgtttt tgactcgatt
     1081 tattttcttg gcggcgcgtg cgcgggcgcc cctagtcccc gcggcttgtg cccgcagcgc
     1141 gtcgatgtgc gcgcgtagga cggccaccga gtcgtcgaac ggcccgatat gccccgcggc
     1201 gcgcgcctgc atcaacccgc cttccatgac ggtcaacacg aaacgcgcaa gccggcgcag
     1261 atccgtgccg ggcggcagct cgtcgccggc gctacgcagc cacccttcca cgcggccggc
     1321 ccagttctcg aaattgcggt ggatcagctc gcgcgctcgc gggttgccgt cgctgacctc
     1381 gagcgccaaa ttgccgatgg ggcaacccat gcggcactga ttcgcaccga cccactcgcg
     1441 atattgattc agcaacacaa agacgcgctc gagcggatcg acggccgcgg cctcggccgg
     1501 gcccatgacc atcggctcga ggtagtcgag cgcgaactcg agcacctcga gcaacaggtc
     1561 gtccttgctc gaaaagaagt ggtacaggct gccggcgttg acgccggcct cacgcaggat
     1621 cgtcgcgaca ccggtcgcgt gataaccctg ctcgtggaac agctggaacg ccgtctttag
     1681 cagacggtcg cgcgtttcaa cggcgaccgg ttcttgttct tgagcggtca ttcaaggcga
     1741 acttaaacga tcgttcaaga atggtcaatg cgcagccggc gccccatcag aggcgccggc
     1801 cggcattgcg agaaggctcg actttagcgg cggctgaccc tgcgatcgcg atcgttatcg
     1861 cggtcgccgt agcactcgag caagaagtcg ttgcccgtcg ttgcgcgcgt gatgcggacg
     1921 tactccgttt catcagggca ctcgaccgtg ttgcggtccg tattcgctgc gccgacgcac
     1981 gtccaatcga tctcgacgtc gtcgttgtcg tcggcatcga tttggaagac ggcgttctgg
     2041 ccgcgctgaa tgacgagatc ggcacgtcgc gcgttcgcaa ccgagcgctc gtcgatcgcg
     2101 accgagcccg agcattcgtt gcggtccgaa tcgagcagcg tgacgcccgt cggcaggcgc
     2161 gcgctgcgcg cgtcctcgtc ggtcgacgtc gtcgaggcac agcccgagcc gagcactgca
     2221 aaagctgcga gataaagcca actctgcggt cgaatctgca tgtctctctc cttgagcggt
     2281 ttctccttcg actactcaag aagcaacaac catgccagcc gacagcagag ctggcgccgg
     2341 cttagtcggt ccagaacatc tcgaggttgc gcgccaacag ggagccgggc accgcgcccg
     2401 ccttcgcctt gtcgcgatgt gcgctcgcga cgcgctgctg cgtcatcaac gacgccgcaa
     2461 actcgatctc gggcaactcc gtcaccggag cttgcgcgcg cgctctacgc aacaacgcat
     2521 agccgtctac ggcgagctga gccctcggca cgatggtcgc gggacgcccg tgcgcgggcc
     2581 gcatctcgta ccgatagatc aagtcgagct ggcgatacgt ctcgagcaga tagccggcgt
     2641 cgaaccacgc catggccgcg gcgcgcgcgt cggcctgcgg cgtcttcgtg cgctcgacga
     2701 cggccgtcaa caacgactcg gcaaggccga gatctcgctc cgcatagatc gcggcgcggc
     2761 gcatgttctc catgcggtcg atgacgcgcg cgtccggcga gagcagcttt agcgtgtcgg
     2821 ccgcgagccg cgcgacgtcg tagctcgggt ccggggagtg ccagtcgcgg gtcggcaacc
     2881 acggcagcat cggcgccgcc gcgtcggtga tgaacggatg acagatcagc ggcggcccgg
     2941 ccagcgcggc ctgcgcaaag ccggcgacgg ccgccagcgt cacgatgact cgaagggggc
     3001 gaagaggaaa catgatgcgg tctcccgaag ggttgcggca catcccgcga gatgcagtgt
     3061 cgtgccccga gtctgctcgc aaggagtcag ttgccgttag ctctaaccgt tagtaacccc
     3121 ctgccggcaa gccaaatcaa cctcggcgcc gacggcaacg ggcggccgtt gtgggagact
     3181 gaggctcgcg tgcaatacgc atgaagc
//
LOCUS       DATLEE010000273         2755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1054659, whole genome shotgun sequence.
ACCESSION   DATLEE010000273 DATLEE010000000
VERSION     DATLEE010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054659"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VM692_05695"
     CDS             <1..487
                     /locus_tag="VM692_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HUQ51693.1"
                     /translation="PWPNPSEVFSLEVFGVSVPLLLIGIMTFDLITSSGTMALAVNFG
                     YRRDRGRAAALMFITAAFGAIFVGMQAFEWSKLILEEGIRPWGNPMGASQFGAAFFMI
                     TGFHGLHVSAGVAYLTIVAMKVLRGDYEKKGYEIVEIAGLYWHFVDLVWVFIFAFFYL
                     W"
     gene            499..846
                     /locus_tag="VM692_05700"
     CDS             499..846
                     /locus_tag="VM692_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HUQ51694.1"
                     /translation="MSEAVGQQHPLKLYFVIWALLFLFSAASYGTDFLPHGYFRWFLI
                     LTFMLLKAGFIIAIFMHMKWERLALIWAILGPPVVLLVLIGLMAVEGDYTELTRLDFF
                     SAPVVPTGHAEAH"
     gene            880..1179
                     /locus_tag="VM692_05705"
     CDS             880..1179
                     /locus_tag="VM692_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51695.1"
                     /translation="MLLWLIGVTVFVLLWGAFWKASLKGGLGVLIGLPVAWILSRLLT
                     PYVTGMEEIPLWVPPLPIACIALALFYFGVRTWLRADNLPPPPQQPDDEHGHGHH"
     gene            complement(1213..1545)
                     /locus_tag="VM692_05710"
     CDS             complement(1213..1545)
                     /locus_tag="VM692_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ51696.1"
                     /translation="MAHDLIANLEALLAKGGDGAALRLALASRYLDVGDAAAAVRHAE
                     AAVRLDPEYSAGWKALGRALTVAGRGADAVTAYERGTEVAERRGDQQAAKEMRVFSAR
                     LKRATPTP"
     gene            1673..2635
                     /locus_tag="VM692_05715"
     CDS             1673..2635
                     /locus_tag="VM692_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HUQ51697.1"
                     /translation="MARTESTLPQLDGGWFLTDGGIETVLIFQEGIALPEFAAFVLLQ
                     SAEGRDRLRNYFRPFLDVAARAPGAGFVLESPTWRAGFNWGAKLGFDGRRMRQFNRDA
                     IALMRDLQVEYAARITRPIVVSGCVGPRGDGYVAGAPMALDDAARVHQPQVDALAAAG
                     ADLITAITMTTSAEAIGVARAAARAGRPAAISFTVETDGTLPSGESLRAAIGAVDDDA
                     ATAGHEPPAYYMLNCAHPCHFEHVLGSLGERRSRLRGLRANASRRSHAELDAATELDA
                     GDARELGADYARLKAHWPSLTVLGGCCGTDHTHVAAMSTALLGG"
BASE COUNT          413 a          935 c          904 g          503 t
ORIGIN      
        1 gccatggccg aaccccagcg aggtgttcag cctcgaagtg ttcggcgtca gcgtcccgct
       61 gctgctcatc ggcatcatga cgttcgacct gatcacgagc agcggcacga tggcgctggc
      121 cgtcaacttc ggctatcggc gtgaccgcgg ccgtgccgcc gcgctgatgt tcatcacggc
      181 ggccttcggc gcgatcttcg tcggcatgca agccttcgag tggagcaagc tgatcctgga
      241 agagggcatc cgaccctggg gcaacccgat gggcgcctca cagttcggcg cggcgttctt
      301 catgatcacg gggttccacg gcctacacgt gtcggccggt gtcgcctacc tcacgatcgt
      361 cgcgatgaag gtgttgcgcg gcgattacga gaagaagggc tacgagatcg tcgagatcgc
      421 gggcctgtac tggcacttcg tcgacctcgt ctgggtcttc atcttcgcgt tcttctatct
      481 ctggtagggg gcatttgaat gtcagaagca gttggccaac agcatccgct gaagctgtat
      541 ttcgtcattt gggcgttgtt gttcctgttc agcgccgcct cctatggaac ggacttcctc
      601 ccgcacggct acttccggtg gttcttgatc ttgacgttca tgctgctgaa ggcgggcttc
      661 atcatcgcga tcttcatgca catgaagtgg gagcgcctcg cgttgatctg ggcgatcctc
      721 gggccgcccg tggtgctgct ggtgttgatt gggctcatgg ccgtcgaagg cgactacacg
      781 gagctcacgc ggctcgattt cttctctgcg cccgtcgtgc ccacggggca tgcggaagcg
      841 cactgattta gcgcggcgaa acaacggagg gcgtgcccga tgttgctttg gctgattggc
      901 gttacggtat tcgtgctgct ctggggcgcc ttctggaagg cgagcctgaa gggcgggctt
      961 ggcgtgctga tcggccttcc ggtcgcatgg attctttccc gattgttgac accctacgtg
     1021 acaggcatgg aagaaatacc tctttgggtt ccgccgttac cgatcgcgtg catcgcgctc
     1081 gcgctgttct acttcggcgt ccggacttgg ctgcgggccg acaacctgcc gccgccgccg
     1141 caacagcccg acgacgagca cggccacggc catcactgag cgccggcgcg ctcgagactc
     1201 cgtcgtcgcc gcttaaggcg tcggcgtagc gcgcttcaag cgcgcgctga acacgcgcat
     1261 ttctttggct gcttgttggt cgccgcgccg ttcggccacc tcggtgccgc gctcgtacgc
     1321 cgtcaccgcg tccgcgccgc ggcccgccac cgtcaacgcc cggcccaacg ctttccagcc
     1381 ggccgaatac tcgggatcga ggcgcacggc cgcttcggcg tggcgcacgg ccgcagccgc
     1441 atcgccgaca tcgaggtaac gcgacgccag cgcgagccgc aacgccgccc cgtcgccgcc
     1501 cttggcgagc agcgcctcga gattagcgat gagatcgtgg gccatggcct gactcatcgt
     1561 acgccgaaaa ccaccggcgg actttcgatc aagaatggcg gccttacggg cattggaccg
     1621 gggccgctcg atgcctacag tgccgaccct tcgccaacga tggagggcgc tcatggctcg
     1681 cactgaatcg actttgccgc agctcgacgg cggctggttc ctgaccgacg gcggtatcga
     1741 gacggttttg atctttcagg agggcatcgc gctgccggag ttcgcggcgt tcgtgctgct
     1801 gcaatcggcc gaaggccgcg accgcctgcg caactacttc cgcccgtttc tcgacgtcgc
     1861 cgcgcgcgca ccgggcgccg gcttcgtgct cgaaagcccg acctggcgcg cgggcttcaa
     1921 ctggggcgcc aagctcggct tcgacggccg ccgcatgcgc cagttcaacc gcgacgcgat
     1981 tgccttgatg cgggatctgc aagtcgagta tgcggcgcgc atcacgcggc caatcgtcgt
     2041 cagcggctgc gtcggaccgc gcggcgacgg ctacgtcgcc ggcgcgccga tggcgctcga
     2101 cgacgcggcg cgcgtccatc aaccgcaggt cgacgcgttg gcggccgcgg gcgccgacct
     2161 gatcaccgcg atcacgatga cgacgagcgc agaggcgatc ggcgtcgctc gcgccgcggc
     2221 acgcgccggc cgacccgcgg cgatctcatt cacggtcgag acggacggca ctttgccgag
     2281 cggcgaatcg ttgcgtgcag ccatcggtgc cgtcgacgac gacgcggcca cagccggcca
     2341 cgagccgccc gcctactaca tgctgaattg cgcacatccc tgccacttcg agcacgtgct
     2401 ggggtcgctc ggcgagcgcc gctcgcggct acgcggcctg cgagcgaacg cgtcgcgccg
     2461 cagccatgcg gagctcgacg ccgcgacgga gctcgatgca ggcgacgcgc gcgagctcgg
     2521 cgccgactac gcacgcttga aagcgcattg gccgtcgctg accgtcctcg gcggctgctg
     2581 cggcaccgac cacacgcacg tcgcggcgat gagcacggcc ctgctcggcg gctgacgcgc
     2641 tcgcggcaat cgacgccgaa gccgcatgct tagctcgccg cgacagggtg gtcgacatgc
     2701 ggcttcggcg aagtgcccgc ccgcttgcgg ttcagatcaa agcaccgccg acctc
//
LOCUS       DATLEE010000274        10905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5526959, whole genome shotgun sequence.
ACCESSION   DATLEE010000274 DATLEE010000000
VERSION     DATLEE010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5526959"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..3574
                     /locus_tag="VM692_05720"
     CDS             287..3574
                     /locus_tag="VM692_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ51698.1"
                     /translation="MNDLIRGWNWSETPLGPTERWPEVLRSTLGLCLDSAFPIAIYWG
                     PELALLYNDAWRPILGNKHPRALGRPAVKVWPEIWDAIEPVFQRVRETRVGAFNGDSL
                     LAMHRHGYVEECYFDYTFNPIRDGAGGVAGIFNVVIETTYRVINERRGKVLREFAAHL
                     AAARSADDVIALAAQALEAAPFDVPCALIYRVDGAEPGAVRLAQSVRVAAPAAFSATT
                     WPIAAALRDGAPQRVADLQTRVGAFSGGAWAEAPREALVLPIHAPGRPAPDAVLVAIA
                     SPRRALDAEYTAFFESLARHMAAAIASAEAFEAARRRAEALAELDRAKTVFFSNVSHE
                     LRTPLALILAPLNDLLAAGHGTLSAGVQESLQLARRNAERLRKLVNNLLEFSRLEAGR
                     VAVRYEQTDLARLTEDLASNFRSACERAGLALRVECPPLPEPVLVDRDMWERVVLNLL
                     SNAFKFTLQGEIFVGLEQAGGRAVLTVRDTGTGIPAEELPRLFERFRRIENAHARSQE
                     GTGIGLALVNELVKLHGGEVVVESAVGRGTQIEVAIPLDRTTLPADTVAGERRPAEGA
                     RTARFVEEALSWLGDEPHGAAPPSRDATAAPRQTVLVADDNADMRAYLARLLGDSYDV
                     IVVARGDEALEVAHRARPDLVLSDVMMPGLDGFALLRALRHDERTRTVPVILLSARAG
                     EEARVEGLEAGADDYLVKPFDSRELVARVGGAIALATQRRDADRRKDEFLATLAHELR
                     NPLAPIRMAAEVLKRAPDAKAEEYARGVIERQVDNMSRLIDDLMDLSRISRGKIELRM
                     ETMDLQSVLQEAIEATQPLLAGANQLLSVHLPSQPIRIRCDRVRLTQVFTNLLNNASK
                     YSNAGARISIDASLDDNDAVVSVTDEGVGIDAAMLPKVWDMFVQAHRSMERSHGGLGI
                     GLTIVRSLVEMQGGSVNASSPGPDRGATFTVRLPLHADVRDSRQTPAQAARVSPASAR
                     VLVADDNEDAAAMLGAYLETMGCVVAVAHDGRAALELAQRFRPDVAVLDLGMPRLNGL
                     ECARELRSESWGRAVKLVALTGWGQEEDRKRSKEAGFDHHLVKPVNPDIVAALIAQQA
                     GSRARQE"
     gene            complement(3620..5362)
                     /locus_tag="VM692_05725"
     CDS             complement(3620..5362)
                     /locus_tag="VM692_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51699.1"
                     /translation="MSKAEQRRVEVPFQRHVELLQSFLAHRDEIVERVQNALNAQRKP
                     AHYLQDARLLARELEDCVFAAAGPSAEPLRRQLDDAHLASGFKPRQAPGEPNDLIDPA
                     ELMTRAFFLWQRTRWPGHDGRTRYAHTLFGLYVLRRLMLLAMRIWDGDAARAAERLAQ
                     LQAVLDELWRTAPADQPRFVRDAHSLFALAQSPTTEELHGYFEVAQRIAETLSQHDRV
                     AIDKAAVRMAGGHLRSQLRHVATQRGVTLDEHSLVLSTRRSNALDLATLVQALVPLLE
                     SYEQAVQRGDAEQRLDLADAICQGISPDPELFLNRVDLLRPYSMIEPLFIAAADGRAA
                     YTPMGERHRRLLDEYAQRIARAAKPLHEDCARFSPRDGAYSPYGVLYGFSSQLLEHMG
                     LKAVQPEAPTRFSLEDVFTSGDADKLAWVNGWRRLPHVPRDVSKLFEYPQPFAAEVFR
                     RIESALRAHVGQENAARRANGRLLVVPQQNGGVAATADLPARYFVSSDRHWCAAHHVP
                     AAEEAQLLHSRTEGELLVCYATSAGWVGVTKDVLTDVLGAGRDAQLGLPPAAARVLQL
                     MCPELVSVAERASP"
     gene            complement(5451..5753)
                     /locus_tag="VM692_05730"
     CDS             complement(5451..5753)
                     /locus_tag="VM692_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HUQ51700.1"
                     /translation="MKTLHQTLLAVTGVLSIASFGARAELPTAARSVEISYAQLDLGT
                     TAARDTLTRQIALAAERACGAYEARSLRERNAWRECRAAAFSAGVAQLAEARVATR"
     gene            complement(5906..7540)
                     /locus_tag="VM692_05735"
     CDS             complement(5906..7540)
                     /locus_tag="VM692_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ51701.1"
                     /translation="MNKPSVYRFAGCEVDSALRQVRCNGVAAEPQPKALDLLLYLIAH
                     RERVVDKDELLEKIWPGAVVSESALTQSLRKARAMVGDDGERQAVIRTIQRRGFRFVA
                     ELDGGSAPHPAPAAAAVTATHAPATTEPVAVLPFVDMSPAADQEYFCDGMAEEVINAL
                     ALAGQRVVARTSSFAFKNRPDDVREIARKLGVTLVLEGSVRKAGDRLRVTAQLIDARS
                     GFHAWSRTWDRRVEDMFAIQDEIAQSIAAALGPTPAASGTKITAAELQRRGRIYQHRF
                     GQRAQRFAIEMFHQAIALDAGYAPAWAALALSYVLLYRYAFASDANRSEALSAAQRAL
                     ELDPKLAEAWAAKGAATTLSRHYRDAEAAFEQAVKLGPNSFEAHYYYARAATETGDFA
                     KAAALYERAATIDADDYQALVFASQCYRSLGQRDRSLEAERRALAAAERALARDSTDA
                     RALVLSAAALIHVGRADDARKWVARGCALEPDEPHCRYNAACAYVLLGEHERALDLLE
                     GLDVASKANGSWMQHDVELDPLRDHPRFKAIMATTR"
     gene            8211..8513
                     /locus_tag="VM692_05740"
     CDS             8211..8513
                     /locus_tag="VM692_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51702.1"
                     /translation="MSCVKLPATVAFPTDDRLLQRIEASRRALPRNNMKPLWHSRDPE
                     CSELRPELRMHEVELQPQPRVDASRGTFERFQAARAELRSTLSHMKQLYPRNRIAS"
     gene            complement(8527..9081)
                     /locus_tag="VM692_05745"
     CDS             complement(8527..9081)
                     /locus_tag="VM692_05745"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HUQ51703.1"
                     /translation="MDYGLVIGLCWLAFFLAWTIGAMVFGGARGRQSSAAAIGIRALL
                     LVTVLASVRYGARLDVRLFGDQAEYFAKAGVALCIVGLTFAIWARIALGRSWGMPMTV
                     HADPELVVRGPYRFVRHPIYTGLTTMFIGTSLVYPLFAISSAVVIAYSVLSALREERD
                     MEQRFPNSYPDYKKRSKMLVPFLL"
     gene            complement(9085..10482)
                     /locus_tag="VM692_05750"
     CDS             complement(9085..10482)
                     /locus_tag="VM692_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HUQ51704.1"
                     /translation="MSVAARVPREIRRASLTCVVALAVALPALGQSTANDHWVATWAT
                     ALVARPLPPPPGAAPPNGAGPPPAAGLVAGPPQAGGPPAAAPPAGAPPAAAPPGGAGP
                     PRPPPPVTVTNQTLRQVVHTSIGGDRVRVVLSNVFGTTPLEVGAAAVALREQGSAVKA
                     NAVRPLTFGGQKAATILAGATLVSDPVELAVAPVSDLVVDLYVPGDLGIKPSPVTTHN
                     GASQTNYVSTPGNHVGMQSLTVDREAGAWFLLARVEVAAAADTRAVVTFGDSITDGAR
                     SSANANARWPDALARRLNEQKRGPKVAVLNAGISGNRVLGDGAGVSALARFDKDVLLQ
                     TGVTHVVVMEGINDIGLARAEATPSADDLIAGHRQLIARAHARGLKIYGATLTPFEGA
                     AYYTPQGEAKRQALNNWIRTSGEYDGVIDFDAVTRDPAAPSKFAAFADSGDHLHPGDA
                     GYKAMGDAVDLALFK"
     gene            complement(10578..>10905)
                     /locus_tag="VM692_05755"
     CDS             complement(10578..>10905)
                     /locus_tag="VM692_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007999982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HUQ51705.1"
                     /translation="VAAVPTANREQYLRHAKEFAEVFKQNGALRVVECWGDDVPMGKT
                     TSFPQAVKLEPNETVAFSWIAWPSRQARDAGMKKAMEDPRMKNAEMPFDGKRMIYGGF
                     EQILDA"
BASE COUNT         1660 a         3723 c         3806 g         1716 t
ORIGIN      
        1 ggcgcgaccg cgggccgcct gatagcggcg atcctcgcgc tggagcaggc gccggacgtc
       61 cgttcgctgc gcccgctgct gcagcggccg ccgcgccgct gagccggcca gcggcgggcc
      121 ggccgcggcg tacaaccgtg gccgttctgc gcgatggccc atgcggcatg tttcttgctt
      181 ttttcgtcgc taacaagcaa gccgataacc gccccaacac gcgcgaacga tgtcgagcga
      241 ccaagtagag ccgccacacc cgagttctat gccggccgcc ggctccatga acgacctcat
      301 ccgaggctgg aattggagcg agacaccgct cggacccacg gaacgctggc ccgaagtgtt
      361 gcgctcgacg ctcggcctgt gcctcgactc cgcgttcccg atcgcgatct attgggggcc
      421 ggagctcgca ctcctttata acgacgcttg gcgcccgatc ctcggcaaca agcacccgcg
      481 ggcgctcggc cggcccgccg tcaaggtctg gccggaaatt tgggacgcga tcgagcccgt
      541 gttccaacgc gtgcgcgaga ctcgcgtagg agccttcaac ggcgacagcc tgctcgcgat
      601 gcatcggcac ggctacgtcg aggagtgcta tttcgactac acgttcaacc cgatccgcga
      661 cggagcgggc ggcgtggccg gcatcttcaa cgtcgtcatc gagacgacgt accgcgtcat
      721 caacgagcgg cgcggcaagg tgctgcgcga gttcgccgcg cacctcgccg cggctcgcag
      781 cgcggacgac gtgatcgcgc tcgcggcgca ggccctcgag gccgcgccgt tcgacgtccc
      841 ctgtgctctg atctatcgcg tcgacggcgc cgagcccggc gccgtgcggc tcgcgcagtc
      901 cgtccgcgtc gccgcgccgg ccgcgttcag tgcgaccact tggcctattg ccgctgcgct
      961 gcgcgacggc gcgccgcagc gcgtcgccga cctgcagacg cgcgtcggcg cgttttcggg
     1021 cggcgcttgg gccgaagcgc cgcgcgaggc actcgtgctg ccgatccacg cgcccggcag
     1081 gcccgcgccc gacgccgtgc tcgtcgcgat cgcgagcccg cgtcgtgcgc tcgacgccga
     1141 gtacacggcc ttcttcgaat cgctggcgcg ccacatggcg gccgcgatcg cgagcgccga
     1201 agcgttcgaa gccgctcgcc gccgcgccga agcgctggcg gagctcgacc gcgcgaagac
     1261 cgtgttcttc agcaacgtca gccacgagct acgcacgccg ctcgcgctga tcctggcccc
     1321 gctgaacgac ctgctggcgg cagggcacgg cacgctgagt gccggcgtgc aagagtcgct
     1381 gcagctcgcg cgccgcaacg ccgagcggct gcgcaagctc gtcaacaatt tgctcgagtt
     1441 ctcgcgcctc gaagcggggc gcgtcgctgt gcgttatgaa cagacggacc tcgcgcgtct
     1501 gaccgaggat ctcgcgagca acttccgctc cgcctgcgag cgtgcgggct tggcgttgcg
     1561 cgtcgagtgc ccgccgctgc ctgagcctgt gctcgtcgac cgcgacatgt gggagcgcgt
     1621 cgttctgaat ctcttgagca atgcattcaa gttcacgctg caaggcgaga tcttcgtcgg
     1681 cctcgagcag gccggcggcc gcgcggtgct gacggtgcgc gacacgggca ccggcatccc
     1741 cgccgaggag ctgccgcggc tgttcgagcg cttccgtcgc atcgagaatg cacacgcgcg
     1801 ctcccaagag ggcaccggca ttgggctcgc gctcgtcaac gagctcgtga agctgcatgg
     1861 cggcgaggtc gtcgtcgaga gcgccgtggg tcgcggcacg cagatcgagg tggcgatccc
     1921 gctcgaccgt acgacgctgc ctgccgacac cgtcgccggc gagcggcggc ccgccgaggg
     1981 cgcgcgcacg gcgcgcttcg tcgaggaagc tctgagctgg ctcggagacg agccgcacgg
     2041 cgcagcgccg ccgagccgtg acgctacggc ggccccgcgt cagaccgtgc tcgttgccga
     2101 cgacaacgcg gacatgcggg cgtacctcgc gcggttgctc ggcgatagct acgacgtcat
     2161 cgtcgtcgcg cgcggcgacg aggcgctcga agtcgcgcat cgggcgcgcc cggacctcgt
     2221 gctgtccgac gtcatgatgc cggggctcga cggcttcgcc ctgctgcgcg ccctgcggca
     2281 cgacgagcgg acgcgcacgg ttcccgtgat tctgctatcg gcccgcgccg gcgaagaagc
     2341 tcgcgtcgag ggcctcgagg ccggcgccga cgactatctc gtgaagccgt tcgactcgcg
     2401 cgagctcgtg gcgcgcgtcg gcggcgcgat cgcgctcgcg acgcaacggc gcgacgcgga
     2461 tcggcgcaaa gacgaattcc tcgcgacgtt ggcgcacgag ctgcgcaacc cgctcgcgcc
     2521 gatccgcatg gcggccgagg tgctgaagcg cgctcccgat gcgaaggcgg aagagtacgc
     2581 gcgcggcgtc atcgagcggc aggtcgacaa catgtcgcgg ctcatcgacg acctcatgga
     2641 cttgagccgc atatcccgcg gcaagatcga gctgcgcatg gagacgatgg atttacagag
     2701 cgtgctccag gaagcgatcg aggccacgca gccgctgctg gccggcgcga accagctgtt
     2761 gtcggtgcac ttgccgtcgc agccgattcg catccgctgc gaccgcgtgc ggctgacgca
     2821 agtgttcacg aatctgttga acaacgcgtc gaagtactcg aacgccggag cgcggatctc
     2881 gatcgacgcc agcctcgacg acaacgacgc cgtcgtcagc gtgaccgacg aaggcgtcgg
     2941 catcgacgcc gccatgctgc cgaaagtctg ggacatgttc gtgcaggcgc accgctcgat
     3001 ggagcgctcg cacggcggtc tcggcatcgg gctcacgatc gtgcgcagct tggtcgagat
     3061 gcagggcggc tcggtcaacg cctcgagccc tgggccggac cgcggcgcga cgttcacggt
     3121 gcggctgccg ttgcatgccg acgtgcgcga ttcgcggcag acgccggcgc aggcagcgag
     3181 ggtgtcgccg gcatcggcgc gcgtgctggt cgcggacgac aacgaggatg ccgccgcgat
     3241 gctcggcgcc tacctcgaga cgatgggctg cgtcgtggcc gtcgcgcacg acggccgcgc
     3301 ggcgctcgag ctcgcgcagc ggttccggcc cgacgtcgcc gttctcgatc tcggcatgcc
     3361 gcggctcaac ggcctcgagt gcgctcgcga gctgcgcagc gagtcgtggg gcagggcggt
     3421 caagctcgtg gcgctgacgg gttggggaca ggaagaggac cgcaagcgct cgaaggaagc
     3481 cggctttgac catcacctcg tgaagccggt gaaccccgat atcgtagcgg cgctgatcgc
     3541 gcagcaggcg gggagccgcg cacgccagga atgaccgagc gccgcgacgc ggcggtgcaa
     3601 ttcgcgcctg cgacgaccat tacggtgacg cgcgctccgc aacgctgacg agctcggggc
     3661 acatcaactg caggacgcgc gcggccgccg ggggtaagcc gagctgcgca tcgcggccgg
     3721 cgccgagcac gtccgtcaac acgtccttcg tgacgcccac ccagccggcc gacgttgcat
     3781 agcagacgag gagctcgccc tccgtccggc tatgcaggag ttgcgcttcc tcggctgcgg
     3841 gcacgtggtg cgcggcgcac cagtgccgat ccgacgacac gaaatagcgc gccggcaggt
     3901 cggcggtcgc cgcaacaccg ccgttttgtt gcggcacgac gagcaaccgg ccgttcgcgc
     3961 gtcgagcggc gttttcttgg ccgacgtgcg cgcgtagcgc cgactcgatc cgccgaaaca
     4021 cttccgcggc gaacggctgc gggtattcga acagcttcga gacgtcgcgc ggcacgtgcg
     4081 gcaagcgccg ccagccgttg acccacgcga gcttgtcggc gtcgccgctc gtgaagacgt
     4141 cctcgaggct gaagcgcgtc ggcgcttcgg gctgcacggc tttgagcccc atgtgctcga
     4201 gcagctgcga cgagaaccca tacagcacgc cgtacggcga gtaggcgccg tcgcgggggc
     4261 tgaagcgagc gcaatcctcg tgcagcggct tggccgcgcg agcgatgcgc tgcgcgtact
     4321 cgtcgagcag gcggcgatgc cgctcgccca tcggcgtata cgccgcgcgg ccgtcggccg
     4381 ccgcgatgaa tagcggctcg atcatgctgt atgggcgcag caagtcgacg cgattcagga
     4441 acagctcggg atccggcgag atgccttggc agattgcgtc ggccaggtcg agccgctgct
     4501 cggcgtcgcc gcgctgcacg gcttgctcgt aggactcgag cagcggcacg agcgcctgca
     4561 ccagcgtcgc gagatcgagc gcgttcgacc tgcgcgtgct gagcacgagg ctgtgctcgt
     4621 cgagcgtcac gcctcgttgc gtcgccacgt ggcgcagctg cgagcgcaga tgaccgccgg
     4681 ccatccgcac ggcggctttg tcgatcgcga cgcggtcgtg ctgcgacagc gtctccgcga
     4741 tgcgttgcgc gacctcgaag taaccgtgca gctcttcggt cgtggggctt tgcgcgagcg
     4801 caaacagcga atgggcatcg cgcacgaacc gcggctgatc ggccggcgcc gtgcgccaga
     4861 gctcgtcgag cacggcttgt aactgcgcga gccgctcggc tgcgcgtgcc gcgtcgccgt
     4921 cccagatgcg catcgcgagc agcatgagcc gccgcagcac gtacaaaccg aacagcgtgt
     4981 gcgcgtaacg cgtgcggccg tcgtgccccg gccagcgcgt gcgctgccac aagaaaaacg
     5041 cgcgcgtcat gagctcggca ggatcgatga ggtcgttggg ctcgccgggc gcttgccgcg
     5101 gcttgaagcc gctcgcgagg tgcgcgtcgt cgagctggcg ccgcaacggc tcggcgctcg
     5161 ggcccgcagc ggcgaacacg caatcttcga gctcgcgcgc cagcaggcgt gcgtcctgca
     5221 ggtagtgcgc cggcttgcgt tgcgcgttca gcgcgttctg cactcgctcg acgatctcgt
     5281 cgcggtgcgc caagaacgac tgcagcagct cgacatgccg ctgaaacggc acttcgactc
     5341 gacgttgctc ggctttgctc atgcaagctg aatagtggcc gagccgcgcc gggccgagca
     5401 acaaccgccg cgcagcatgg agtgctgcgc ggcggctcgg tgtgcgcgcg ttaacgcgtc
     5461 gcgacgcggg cttcggcgag ttgcgcgacg cctgccgaga acgccgcggc gcggcactcg
     5521 cgccacgcat tgcgctcgcg caagctgcgc gcctcgtagg cgccgcacgc ccgctcggcg
     5581 gccagcgcga tctgccgagt cagcgtgtcg cgcgcagccg tggtgccgag gtcgagctgc
     5641 gcgtagctga tttcgaccga tcgggccgcg gtcggtagct cggcccgcgc gccgaaggat
     5701 gcgatcgaga ggacgccggt cacggcgagc aaggtctggt gcagggtctt catggtcggt
     5761 gctcctggtt ggtggctgtt gccggggcat cccggcttgg cgcacaggat cgcagcgcgg
     5821 ccttaggcgc gcttgaccgc gccgtgatca gttgctgact tattcatgac cgcgccgttg
     5881 ggtcgaagag gtgtgaacgc gccggctagc gcgtggtcgc catgatggcc ttgaaacgcg
     5941 gatggtcgcg cagcgggtcg agctcgacgt cgtgctgcat ccaggagccg ttcgccttgc
     6001 tggcgacgtc gagcccctcg agcaggtcga gagcgcgctc gtgctcgccg agcagcacgt
     6061 acgcgcacgc tgcgttgtag cggcaatgcg gctcgtcggg ctcgagcgcg cagccgcgcg
     6121 cgacccactt gcgcgcgtca tcggctcgcc caacatgaat cagcgccgcg gcgctcagca
     6181 cgagcgctcg tgcatcggtc gagtcgcgcg cgagcgcgcg ctccgccgcc gccagcgcac
     6241 gccgttccgc ctcgaggctg cggtcgcgct ggccgagcga gcggtagcac tggctcgcga
     6301 acacgagcgc ctgatagtcg tcggcgtcga tcgtcgccgc tcgctcgtac agcgccgcgg
     6361 ctttggcaaa gtcaccggtc tcggtcgcgg cacgcgcgta gtaatagtga gcctcgaacg
     6421 agttgggccc gagcttcacg gcttgctcga atgccgcttc ggcgtcgcgg tagtggcggc
     6481 tcagcgtcgt cgccgcacct ttcgcggccc acgcttccgc gagcttggga tcgagctcga
     6541 gcgcgcgctg agcagccgag agcgcttccg agcggttcgc gtcgctggcg aacgcgtagc
     6601 ggtagagcag cacgtagctc agcgcgagcg cggcccacgc cggcgcgtag ccggcgtcga
     6661 gcgcgatggc ctgatggaac atctcgatcg cgaagcgctg cgcgcgctgc ccgaagcggt
     6721 gttgatagat acggccgcgg cgctgcagct cggccgccgt gatcttcgtg ccgctcgcgg
     6781 ccggagtcgg ccccagcgcc gccgcgatgc tctgcgcgat ctcgtcctgg atcgcgaaca
     6841 tgtcttcgac gcgccgatcc cacgtcctcg accacgcgtg aaagccgctg cgcgcgtcga
     6901 tgagctgcgc cgtcactctc agccggtcgc cggccttgcg cacgctgcct tcgagcacga
     6961 gcgtgacgcc gagcttgcgt gcgatctccc gcacgtcgtc gggccggttc ttgaaagcga
     7021 acgacgacgt gcgtgcgacg acgcgttggc cggcaagcgc gagtgcgttg atgacttcct
     7081 cggccatgcc gtcgcagaag tattcctggt cggcggcggg gctcatgtcg acgaacggca
     7141 gcaccgccac gggctccgtc gtcgcgggcg cgtgcgtcgc ggttacggcc gcggccgccg
     7201 gagcggggtg cggcgccgag ccgccgtcga gctcggctac gaagcgaaag ccgcggcgct
     7261 ggatcgtgcg tatcacggct tggcgctcgc catcgtcgcc gaccatcgcg cgcgctttgc
     7321 gcagcgactg cgtcagcgcc gattcggaga cgacggcgcc gggccagatc ttctcgagca
     7381 gctcgtcttt atcgacgacg cgctcgcggt gagcgatcag gtacagcagc agatcgagcg
     7441 ctttcggctg cggctcggcc gcaacgccgt tgcagcgcac ttgccgcagc gccgagtcaa
     7501 cttcgcaacc cgcaaatcga tagacggacg gtttgttcat ggcgtggaag cggggactgc
     7561 cgcccgcgcg ccagtatagg ccgaaccgcg cgaagggagc ccgcagatcg cgggctccct
     7621 cgaagtgcac cgagccggct acgccttaca acgaccgggc gtcatgtgcc tggcatggac
     7681 ttgtagcctg tgcgccaaat gcctcaggta cgccacgctg ccgagccacg agccgtgctt
     7741 ttgcccgctc tcgtgcgcca cttgcgcgac cgagccgagg ccatgaaggc tggacccgac
     7801 gtccttcgcg cccgccgcga gcggcagcgc gaggagtaag gcagccacag ctgctgtctt
     7861 cacgttgatg tctcccttgc ccctgacttg ctctacacag caagaaccat gccgcgcccg
     7921 cttttgcgcc cgcggccgag cgcgagagtc gtgttgcagt tgcaaacttt gaaatgcaca
     7981 aatccccgtt ctcgccgacg tagcgtgcga aaactgtcga aattttcgac acgcatgcgc
     8041 gagcgtcggc ctagttgttg agctgccacg gcactgaccg tcgcgggcct gctttttgca
     8101 ttcactacta gtggggattt ctatgttttc aagcgcacgg acgcgccagt taagggaggt
     8161 tcgtccagat gttcactttg cgaggccgag atccgttcgg ctgtcacggc gtgtcttgcg
     8221 tcaagcttcc ggccaccgtt gcatttccga ccgacgatcg actgctgcag cgcatagagg
     8281 cgtcgcggcg tgcgctgccg cgcaacaaca tgaaaccgct ttggcacagt cgcgacccgg
     8341 aatgcagcga actgcggccc gagctgcgga tgcacgaggt tgaattgcag ccgcagccgc
     8401 gagtcgacgc ttcacgcggg accttcgaac ggtttcaggc agcgcgcgcc gagttacgct
     8461 cgacgctgtc gcacatgaaa cagctgtatc cgcgcaaccg gatcgccagc tagcgctggg
     8521 ttcagctcag agcagaaacg gcacgagcat cttcgagcgc ttcttgtagt ccggataact
     8581 attcggaaag cgctgctcca tgtcgcgctc ctcgcgtagc gcgctgagca cgctatacgc
     8641 gatcacgacg gccgacgaga tcgcaaacag cgggtacacg agcgacgtgc cgatgaacat
     8701 cgtcgtgaga cctgtgtaga tcggatgacg cacgaagcga tacggcccac gcacgacgag
     8761 ctcgggatcc gcgtgcaccg tcatcggcat gccccagctg cggccgagcg cgatgcgagc
     8821 ccaaattgcg aacgtgaggc cgacgatgca gagggccacg ccggccttgg cgaagtattc
     8881 cgcttgatcg ccgaataggc gcacgtcgag cctggctccg taccggaccg acgccagcac
     8941 cgtcacgagc agcagggccc gaataccgat cgcggccgcc gaagactggc gcccgcgcgc
     9001 gccgccgaac accatcgcgc cgatcgtcca cgcaaggaag aacgcgagcc agcaaaggcc
     9061 gatgacgagc ccgtaatcca cgggttactt gaacagcgcg agatcgacgg cgtcgcccat
     9121 ggccttgtag cccgcatcgc cgggatgcaa gtgatcgccg gagtcggcga acgccgcgaa
     9181 cttgctggga gccgccggat cgcgcgtcac ggcgtcgaag tcgatgacgc cgtcgtattc
     9241 gccgctcgtg cggatccagt tgttcaaagc ctggcgcttg gcctcgcctt gcggcgtgta
     9301 gtaagccgcg ccttcgaacg gcgtcagcgt cgcgccgtag atcttgagac cgcgtgcgtg
     9361 cgcacgcgcg atgagctggc ggtggccggc gatcagatcg tcggcgctcg gtgtcgcttc
     9421 tgcgcgcgcg aggccgatgt cgttgatgcc ttccatgacg acgacgtgcg tgacgccggt
     9481 ctgcagcaat acgtccttgt cgaagcgcgc gagcgcgctg acgccggcgc cgtcgccgag
     9541 cacgcgattg ccgctgatgc cggcattcag cacggcgacc ttcgggccgc gcttctgctc
     9601 gttgaggcgg cgtgcgagcg cgtcgggcca acgcgcattc gcgttggcgc tcgagcgcgc
     9661 gccgtccgtg atcgagtcgc cgaacgtgac gacggcacgc gtgtcggccg cggctgcgac
     9721 ctcgacgcgt gcgagtagga accacgcgcc ggcctcgcgg tcgacggtca ggctttgcat
     9781 gccgacgtga ttacccggcg tcgacacgta gttggtttgc gaagcgccgt tgtgcgtcgt
     9841 gacgggcgac ggcttgatgc cgagatcgcc gggcacgtag aggtcgacga cgaggtccga
     9901 gaccggtgcg actgcaagct cgacgggatc gctgacgagc gtggcgccgg ccaagatcgt
     9961 cgcggccttt tggccgccga acgtgagtgg ccgcacggca ttcgctttga cggcggagcc
    10021 ttgctcacgc agcgcgaccg cggccgcccc gacttcgagc ggcgtcgtgc cgaagacgtt
    10081 gctcaacacg acgcgaacgc ggtcgccccc gatgctcgtg tgcacgactt gccgcagcgt
    10141 ttgattcgtg acggtgacgg gcggcggcgg acgcggcggg ccagcgccgc cgggcggtgc
    10201 tgcggccggc ggtgctcccg ccggcggtgc tgcggccggc ggcccaccgg cttgcggcgg
    10261 gcctgcgacc agaccggcgg cgggtggcgg cccagctcca ttcggcggcg cggccccggg
    10321 cggcggcggc aacgggcgcg cgacgagcgc ggtggcccac gtggctaccc agtggtcgtt
    10381 cgccgtcgac tgacccagcg ccggcagcgc gacggccagg gcgacgacgc aagtgagtga
    10441 cgctcggcga atctcgcgcg gaacgcgtgc ggcaacggac atgtggcccc catcgaagaa
    10501 gcttgcgcgg ctcgcaagcg tcagcgattg aagccctgca agggcgccgt gtcaattcgc
    10561 cgccggccgg cggcgcgtca ggcgtcgagg atctgctcga agccgccgta gatcattcgc
    10621 ttgccgtcga acggcatctc ggcattcttc atgcgcgggt cttccatggc cttcttcatg
    10681 ccggcgtcgc gcgcttgccg cgacggccag gcgatccacg agaacgcgac ggtctcgttc
    10741 ggctcgagct tcacggcttg cggaaacgat gtcgtcttgc ccatcggcac gtcgtcaccc
    10801 cagcactcga cgacgcgcag cgccccgttt tgcttgaaca cttcggcgaa ctccttggcg
    10861 tgtcggaggt attgctcgcg gttggcggtc ggaactgcgg cgacg
//
LOCUS       DATLEE010000275         8104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9845578, whole genome shotgun sequence.
ACCESSION   DATLEE010000275 DATLEE010000000
VERSION     DATLEE010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9845578"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VM692_05760"
     CDS             complement(<1..210)
                     /locus_tag="VM692_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HUQ51706.1"
                     /translation="MAKSKPTEEELDELREAFDYNDRDGDGRIQLAEFSDMLDELEAQ
                     MTENEIEIGFQDIDTDDDGRIDFQEF"
     gene            complement(241..1686)
                     /locus_tag="VM692_05765"
     CDS             complement(241..1686)
                     /locus_tag="VM692_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ51707.1"
                     /translation="MPAGQFSKDPVVDKALRHSVRDGMAYAVQVGAGETYFSAFALFL
                     RATAPQIAFVSTLPLLLASFAQIFSAWIGDYVGRRRVVLLGCMLQSLLWLPILLLPML
                     LSEYAVPALLALLVLYHSANNLAAPQWTSIMRDLVSERRRGRYFAHRTRLTTIATFVS
                     LVLCGTLLHELDTANRTYLGFVLIFLIAFVARAISVYHLTFLHEAPRTTTAPDMHIEH
                     WWRSLRSTGAIGFSVYVALMNAAVGISSPFFTVYMLRDLALSYFEFTVLAGTSVLCQF
                     LMLTTWGRIADVYGNRLILIVTSISLPIVPSIWLLTDDFWALLLFQALSGLSWSGFTL
                     SAGNLLYELVPQTRRAAYVAFHNVGTAAGVFGGAMLGALLAELLPPRGVLFGESATLS
                     NLLYLFILSGIARAIVAALLARRVRELRKPRRALSAPALVLRVTGLNAMVGFIYDFIG
                     RPLNDGEERDDAPAVPAAAEAPGDKKGDNTA"
     gene            complement(1803..2294)
                     /locus_tag="VM692_05770"
     CDS             complement(1803..2294)
                     /locus_tag="VM692_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HUQ51708.1"
                     /translation="MAKPMKIDIGIAAKDREVIAEGLSRLLADSYTLYLQSHNFHWNV
                     TGPMFQTLHVLFMEQYTEQWNALDLIAERIRSLGLPAPGTYKEFRRLASIEEVDGTPS
                     ATNMIRILVAGQEAVAKTARAAFEAAESANDQPTCDLLTQRMQVHEKNAWMLRSLLET
                     DES"
     gene            complement(2326..3063)
                     /locus_tag="VM692_05775"
     CDS             complement(2326..3063)
                     /locus_tag="VM692_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HUQ51709.1"
                     /translation="MTQPQEGKRVPNVTFRTRTGNEWRNITTDEIFKGKTVVVFSLPG
                     AFTPTCSSTHVPGFNELAPAFRANGVDRIVCISVNDAFVMNEWAANQGAEEILFLPDG
                     NGEFTAGMGQLVDKGDLGFGKRSWRYSMLVRDGVVEKMFVEPNKPGDPFEVSDAATML
                     AYINPQARKPDQVAILTRDGCQYCAQAKSLLDDRGIDYVDIPLAHKVRNKALGAIAGA
                     QTVPQVFINGQRIGGLEELKRWAARAA"
     gene            3170..4075
                     /locus_tag="VM692_05780"
     CDS             3170..4075
                     /locus_tag="VM692_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HUQ51710.1"
                     /translation="MTLTELKYLIAVADEQHFGRAAERSFVSQPSLSAAIKNIEQELG
                     VRVFERSKRGVELTQVGAEIVAQARRTLDEAARIKTVAKQGKGRYRLRLGVIHTIAPY
                     LLPQLVVALRKLAPDMSLDIEENMTTNLDRLLKAGELDAAILALPYEAPGIEVEPLYD
                     EEFRVVVPQRHAFARRRTIPVEELDAGELLLLPVGHCLRDQVLGACTEFSRSPPPGRL
                     GNSLETLRSMVASGLGITVLPATALSERYETPLVKAVPFAEPKPKRRVAVAWPKNLAR
                     PNAVKKLVEAIRTVDLPVELLGRAQ"
     gene            complement(4055..4573)
                     /locus_tag="VM692_05785"
     CDS             complement(4055..4573)
                     /locus_tag="VM692_05785"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ51711.1"
                     /translation="MQAVTSKLARGALLISFAAYCAACTTLSAPSRPAPTNVPRPPPG
                     AGSPSAPAPAPLPPSTRPPPRSEPLPAPPRGDLPPSRPQSDASGASGALLEQSRAQRA
                     AGSLPAARASLERALRLDPNSPVLWLELGELELQVGNNAQAATLARKAMTLAGRDTRI
                     TARAERLLRAAE"
     gene            complement(4573..6828)
                     /gene="mrcB"
                     /locus_tag="VM692_05790"
     CDS             complement(4573..6828)
                     /gene="mrcB"
                     /locus_tag="VM692_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HUQ51712.1"
                     /translation="MVLRLTAVGFMLAIAYGGYLAWTATSEFEGRRWDIPAQVYAAPL
                     ELYAGRALSAEDLVAELKRLGYREDPRLQHPGTYRLGLGRLELATRGFSFAGDTEPEQ
                     LVSIAFVGGRIATLRDSRGGSTAIVRLNPLLIGSLFPSHGEDRLIVTPDEIPPLLTDT
                     LKAVEDRRFDSHFGIDPIAVARAMFVNVTSGEIREGASTLTQQLVRSYFLTNARSGFA
                     AWWRKIREAFMAVALELRYDKRELLNAYVNEIYLGQDGARAIHGFGLASQFYFGKPLG
                     ELELHELALLVAVVRGPAYYDPRRHADRALERRNFVLQRMVDEGLANNDDVQKAVERA
                     LGLVGDNRRVATHSAFLGLVRRQLSSDYARDDLERTGLIVLSTLDPAVQAASERALAN
                     GLDALGKPAAEFEGAVVVTSPHTGEVKALVGGRDASFEGFNRALDARRQIGSLIKPAV
                     YLTALESGRYSLASIIDDAPIVVQLDKRNTWTPSNFDDQAHGLVPLVRALAESYNMAT
                     VRLGLDVGLEEIADTLQRLGLQQKPTLYPSMLLGALALTPIEVAQIYNTLANGGFRVP
                     LRAVRSVVAEDGKLLQRYEIEIAQAADPGAVYALNQALVQVMERGTGRTVRRQLPDDL
                     VVAGKTGTSDDLRDSWFAGFTNDHLIVTWIGADDNRPVNLTGSTGAAKIWAGVLSSLE
                     ANSYRAPPPEGVSETWIDYATGNPLAERCPDAIPVPVPDTDYYPRVFGCNGETGLGSR
                     VRSWFGGRDPQ"
     gene            7028..7774
                     /locus_tag="VM692_05795"
     CDS             7028..7774
                     /locus_tag="VM692_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HUQ51713.1"
                     /translation="MTEGRPHQIKGKVTSATIVRRVDLTREHFKIWLRCAEPLPFEPG
                     QYCTVGVAGVERPYSIVSSPREPLLELFVELIPPPLGHLTPLLYDLKVGDAVTLRPRA
                     KGVFLLRPQFRNHVMVATVTGIAPFVSMLRHWLEGPVDESRMYVLEGASFRDEFGYDE
                     ELHALAQRHASVRFVPTCSRPDDPRNVGWQGEVGRVHTIVERYVRDWELQPAETCIYA
                     CGHPQMIEDLRGRYAGTGFEFEEERFWKPH"
     gene            7852..>8104
                     /locus_tag="VM692_05800"
     CDS             7852..>8104
                     /locus_tag="VM692_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51714.1"
                     /translation="MKRHWHRNLRWAPLSLTALLATACGGHGGDDAAGAGPPGSVSSG
                     WVAGSFLPAASFAGRCVNPRAGTNPSGGIDTPGTTLDQNN"
BASE COUNT         1381 a         2619 c         2741 g         1363 t
ORIGIN      
        1 gaactcttgg aagtcgatgc ggccgtcgtc gtccgtgtcg atgtcctgaa agccgatttc
       61 gatctcgttc tccgtcatct gggcctcgag ctcgtcgagc atgtccgaga actcggcgag
      121 ctgaattcgc ccgtcgccgt cgcgatcgtt gtagtcgaaa gcttcacgta gctcatcgag
      181 ctcttcttcg gtcggtttgc ttttcgccat gcgtcttaaa gctcctcgga ccaaattcgg
      241 tcaggccgta ttgtcgcctt ttttgtcccc gggtgcctcg gcggcggcag gtaccgcggg
      301 tgcgtcgtca cgctcctcgc cgtcgttcag cggacggccg atgaagtcgt agatgaagcc
      361 gaccatcgcg ttcagccccg tcactcgcag gacgagcgct ggcgcggaca acgcgcggcg
      421 cggcttgcga agctcgcgga cgcgcctcgc tagcaacgct gccacgattg cgcgtgcgat
      481 gccggagagg atgaatagat agagcaggtt gctcagcgtc gcagactcgc cgaacaagac
      541 gccgcgcggc ggcagcaact cggcgagcag tgcgccgagc atcgcaccgc cgaatacgcc
      601 ggcagccgtg ccgacgttgt ggaacgccac gtacgcggcg cgccgcgttt gcggcacgag
      661 ctcgtacagc aagttgccgg ccgacagcgt gaacccggac cagctcaagc ccgacagcgc
      721 ttgaaacaag agcagtgccc agaagtcgtc cgtcagcagc catatcgacg ggacgatcgg
      781 caacgagatg ctcgtgacga tcagtatcag ccgattgccg taaacgtctg cgatgcgccc
      841 ccacgtcgtc agcatcagaa actggcacag cacgctcgtt cccgcgagca ccgtgaactc
      901 gaagtagctg agtgcaaggt cgcgcagcat gtagacggtg aaaaacggcg acgagatgcc
      961 gacggccgca ttcatcagcg ccacgtagac ggagaagccg atcgcgccgg tcgacctcaa
     1021 gctgcgccac cagtgctcga tgtgcatgtc gggcgccgtc gtcgtgcgcg gcgcctcgtg
     1081 caagaacgtc aagtgataca ccgagatcgc gcgtgcgacg aatgcgatca agaaaatcag
     1141 cacgaacccg aggtaggtgc ggttggccgt atcgagctcg tgcagtaagg tgccgcagag
     1201 cacgaggctg acgaacgtgg cgatcgtcgt gagccgcgtg cggtgcgcga aatagcggcc
     1261 gcgccggcgt tcggacacga ggtcgcgcat gatgctggtc cattgcggcg cggccaagtt
     1321 gttcgcgctg tgatacagca cgagcagcgc gagcagcgcc ggcacggcgt actcgctgag
     1381 caacatgggc aacagcagga tcggcagcca aagcagggat tggagcatgc agccaagcag
     1441 cacgacccgg cgccggccga cgtaatcgcc gatccatgcg gagaaaattt gcgcgaacga
     1501 ggcgagcagc agcggcaacg tgctgacgaa cgcgatttgc ggcgccgttg cgcgcaggaa
     1561 cagcgcgaag gccgaaaaat aggtctcgcc ggcgccgact tggaccgcgt acgccatgcc
     1621 gtcgcgcacg gagtggcgca gcgccttgtc gacgacgggg tccttcgaaa actggcccgc
     1681 aggcatacct acacggcgga cgcgcagggc tcgtccatca ctcctcgctg ctgatcagcc
     1741 ggcggcggga gccgccggct ttcggcggga ttataggtcg cgtcgggcgg gaaggaacgt
     1801 gcttagctct cgtcggtttc gagcaggcta cgcagcatcc aggcgttctt ctcgtgcacc
     1861 tgcatgcgtt gcgtcagcag gtcgcaggtc ggctgatcgt tggccgactc ggcggcctcg
     1921 aaagccgcgc gcgccgtttt cgccacggcc tcctggcccg cgacgagaat gcggatcatg
     1981 ttcgtcgcgc tcggcgtgcc gtcgacttct tcgatcgagg ccagccgccg gaactccttg
     2041 taggtgccgg gcgcgggcaa gccgagggaa cgaatgcgct cggcaatcag gtcgagcgca
     2101 ttccactgct cggtgtattg ctccatgaac agcacgtgca acgtttgaaa catcggccca
     2161 gtgacgttcc agtgaaagtt gtgcgactgc agatacagcg tgtagctgtc ggccaagagg
     2221 cgtgacagcc cctccgcgat cacctcgcgg tctttggctg cgatgccgat gtcaatcttc
     2281 atgggcttgg ccatagcagc tccgctgtat ccagtcgcac cagtcttaag ccgcgcgtgc
     2341 ggcccaccgc ttcagctctt caaggccgcc gatgcgttgg ccgttgatga acacctgcgg
     2401 cactgtttgc gcgccggcga tggcacccag cgccttgttg cgcaccttgt gagcgagcgg
     2461 aatgtcgacg tagtcgatac cgcggtcgtc caacaagctc ttcgcctgcg cgcaatactg
     2521 gcagccgtcg cgcgtcagaa tcgcgacttg gtcaggcttg cgagcctgcg gattgatgta
     2581 ggcgagcatc gtagccgcgt ccgatacttc gaacgggtcg ccgggcttgt tcggctcgac
     2641 gaacattttt tccactacgc cgtcgcggac cagcatcgaa tagcgccacg agcgcttgcc
     2701 gaagccgaga tcgcccttgt cgaccagctg ccccatgccg gccgtgaact cgccgttgcc
     2761 gtccggcaag aacaagatct cctcggcacc ctgattcgcc gcccactcgt tcatgacgaa
     2821 cgcgtcgttg acggaaatgc agacgatgcg gtcgacgccg ttggcgcgga atgccggtgc
     2881 gagctcgttg aatcccggga cgtgcgtgct cgagcacgtc ggcgtgaacg ctccgggcag
     2941 cgagaagacg acgacggtct tacctttgaa gatctcgtcg gtcgtgatgt tgcgccactc
     3001 gttgccggtc cgagtgcgga acgtgacgtt cggaactctc tttccttcct ggggctgggt
     3061 catgacatac ctcctgtcga tgagcttcac tttaggggct cgccctatgg cgcggccaat
     3121 actttgttag tatccgatcg atagacaaaa cctatgatgc gaatcccgca tgacgttgac
     3181 tgagctgaag tatctgatcg ccgttgccga cgagcagcac ttcggccggg ccgccgagcg
     3241 ctcgttcgta agccagccca gtctgtcggc cgccatcaag aacatcgagc aagagctagg
     3301 cgtgcgcgta ttcgagcgca gcaagcgtgg cgtcgagctg acgcaggtcg gcgccgagat
     3361 cgtcgctcag gcacggcgta cgctcgacga ggccgcgcgc atcaagaccg tggcgaagca
     3421 gggcaaaggg cggtatcgcc ttcgcctcgg cgtcatccac acgatcgcgc cgtacttgct
     3481 gccgcagctc gtcgtcgcgc tgcgcaagct tgcgccggac atgtcgctcg acatcgaaga
     3541 gaacatgacg acgaatctcg atcgcttgct caaagcaggc gagctcgatg ccgcaattct
     3601 cgcgctgcct tacgaagcgc ccggtatcga agtagagcct ttgtacgacg aagagttccg
     3661 ggtcgtcgtc ccgcagcgtc atgcattcgc acgcaggcgc acgattcccg tcgaggagct
     3721 cgatgcgggt gagctattgc tgctgccggt cgggcattgc ttgcgcgatc aagtgctcgg
     3781 cgcgtgcacg gagttctcgc gctcaccgcc gccgggccgc ttgggcaatt cgctcgagac
     3841 gttgcgcagc atggtggcat cgggcctcgg catcacggta ttacccgcga cggcgctcag
     3901 cgagcgttac gagacgccgc tcgtgaaggc cgtgccgttc gcggagccga agccgaagcg
     3961 gcgcgtcgcg gtcgcgtggc cgaagaatct cgcgcggcct aacgccgtca agaagcttgt
     4021 cgaagcgatc aggaccgtcg atttgcccgt cgagctactc ggccgcgcgc agtagccgct
     4081 cggcgcgcgc cgtgatgcgc gtgtcgcggc ccgcaagcgt catggccttg cgcgcgagcg
     4141 tcgccgcttg agcgttgttg ccgacctgca gctcgagctc gccgagctcg agccacagca
     4201 cgggactgtt gggatcgagc cgcaacgcgc gctcgagaga agcgcgcgcc gccggcaggc
     4261 tgcccgcggc gcgttgcgcg cggctctgct cgagcagtgc gccgcttgcg ccgctggcgt
     4321 cgctctgcgg gcgcgacggt ggaaggtcgc cgcgcggcgg cgcgggtagc ggctcggagc
     4381 gaggtggagg tcgcgtcgac ggcggcagcg gcgcgggagc cggtgccgac ggcgagcccg
     4441 ctcccggcgg cggtcgcggc acgttggtcg gcgcggggcg cgacggagcg ctcagcgtcg
     4501 tgcaggccgc gcagtacgcg gcgaacgaga tcagcagcgc gccacgcgcg agcttcgaag
     4561 tcacggcttg catcactgtg ggtcgcggcc gccgaaccac gagcggacgc gcgagccgag
     4621 cccggtctcg ccgttgcagc cgaacacgcg cggatagtaa tcggtgtcgg gcacggggac
     4681 cggaatcgcg tcggggcagc gctcggccag cggattaccc gtcgcataat cgatccaggt
     4741 ctcgctgacg ccttcgggcg gcggcgccct gtaagagttg gcctcgagcg acgacagcac
     4801 gccggcccag atcttggcgg cccccgtcga gccggtcagg ttgaccggcc ggttatcgtc
     4861 ggcgccgatc caggtcacga tcagatggtc gttcgtgaag ccggcgaacc agctgtcgcg
     4921 caagtcgtcc gaggtgccgg tcttgcctgc gacgacgagg tcgtccggta attgccggcg
     4981 caccgttcgc ccggtgccgc gctccatgac ttgcacgagc gcttggttca gcgcatagac
     5041 ggcgccggga tcggccgcct gtgctatctc gatctcgtag cgctgcaaga gcttaccgtc
     5101 ctcggcgacg acgctgcgca cggcgcgcag cgggacgcga aagccgccgt tcgcgagcgt
     5161 gttgtagatc tgcgcgactt cgatcggcgt cagcgccagc gcgccgagca gcatcgacgg
     5221 gtagagcgtc ggcttctgct gtagcccgag ccgctgcaac gtgtcggcga tctcctcgag
     5281 ccccacatcg aggccgagcc gcacggtcgc catgttgtaa gactcggcga gcgcgcgcac
     5341 gagcggcacg aggccgtgcg cctggtcgtc gaagttgctc ggcgtccacg tgttgcgctt
     5401 gtcgagctgc acgacgatcg gcgcgtcgtc gatgatgctg gccagcgaat agcggccgga
     5461 ctcgagcgcc gtcaggtaga cggcgggctt gatcagcgag ccgatctgcc ggcgcgcgtc
     5521 gagcgcgcgg ttgaaccctt cgaagctcgc gtcgcggccg ccgacgagcg ccttgacctc
     5581 gcccgtgtgc gggctcgtga cgacgacggc gccctcgaac tccgccgcgg gcttgccgag
     5641 cgcgtcgagg ccgttcgcca acgcgcgctc cgacgcggcc tgcacggcgg gatcgagcgt
     5701 cgacagcacg atgagcccgg tgcgctcgag gtcgtcgcgc gcgtaatcgg acgacagttg
     5761 ccggcgcacg agccccaaga acgcggagtg cgtcgcaacc cggcggttgt cgccgaccaa
     5821 gccgagcgcg cgctcaaccg ctttctgcac gtcgtcgttg ttcgcgaggc cctcgtcgac
     5881 catgcgctgc agaacgaagt tgcgccgctc gagcgcgcgg tccgcgtgcc gccgcgggtc
     5941 atagtaagcg ggcccacgca ccacggcgac gagcaatgcc agctcgtgca gctcgagctc
     6001 gccgagcggc ttgccgaagt agaactggct cgcgagcccg aacccgtgga tcgcgcgcgc
     6061 cccgtcttgg ccgagataga tttcgttgac gtaggcgttc agcagctcgc gcttgtcgta
     6121 gcgcagctcg agcgccaccg ccatgaaagc ttcgcggatc ttgcgccacc aggccgcgaa
     6181 gccgctgcgc gcgttcgtca agaagtaact gcgcacgagc tgttgcgtca gcgtgctggc
     6241 cccctcgcgg atctcgccgg aggtgacgtt gacgaacatc gcacgcgcga cggcaatcgg
     6301 atcgatgccg aagtgactgt cgaagcggcg gtcctcgacg gccttcaacg tatcggtcag
     6361 cagcggcgga atctcgtcgg gcgtgacgat gagccggtcc tcgccgtgcg acgggaacag
     6421 gctgccgatc aaaagggggt tcaagcgcac gatcgcggtc gagccgccgc gcgagtcgcg
     6481 cagcgtcgcg atccggccgc cgacgaacgc tatcgagacg agctgctcgg gctccgtatc
     6541 tccggcgaac gagaagccgc gcgtcgcgag ctcgaggcgc cccaagccca accgataggt
     6601 gccggggtgt tgcagccgcg gatcctcgcg atacccgagc cgcttgagct ccgccacgag
     6661 gtcttcggcc gacagcgcgc ggccggcgta cagctcgagc ggcgcggcat agacttgcgc
     6721 cggaatgtcc caccggcgcc cttcgaactc gctcgtagcc gtccatgcca ggtagccgcc
     6781 gtaggcgatc gccagcatga agccgacggc cgtgaggcgt aggaccaagc cgcggatacg
     6841 ggagagcttc gagtttttta aggtacggcg cgaggggcgg gccatgccgg gcacctttgc
     6901 atgagttggc ccgcacatca agccgttaca gccgtttgtg caagacacga gacgagcacg
     6961 ggtgtgcgtg ggagtaccat gcaagcgttg ccgagctcgg acgtgctcgc ggccgcggag
     7021 ggaatacgtg actgaaggcc gaccgcatca gatcaagggc aaagtgacga gcgcgacgat
     7081 cgtgaggcgc gtcgatttga cgcgcgagca cttcaagatt tggttgcgct gcgccgagcc
     7141 gttgccgttc gaaccgggtc agtactgcac ggtgggcgtc gccggcgtcg agcgcccgta
     7201 ctcgatcgtc tcctcgccgc gcgagccgct gctcgagctc ttcgtcgagc tgattccgcc
     7261 gccgctcggt cacctgacgc cgctgcttta cgacctgaag gtcggcgacg cggtgacgct
     7321 acggccgcgc gcgaagggcg tctttttgct gcgcccgcaa tttcgcaacc acgtgatggt
     7381 ggcgacggtc accggcattg cgccattcgt cagcatgctg cgtcattggc tcgaagggcc
     7441 cgtcgacgag agccgcatgt acgtgctcga aggtgcgagc tttcgcgacg agttcggcta
     7501 cgacgaggag ctacacgcgt tggcgcagcg ccacgccagc gtgcgtttcg tgccgacgtg
     7561 ctcgcggccc gacgatccgc gcaacgtcgg ttggcagggc gaggtaggcc gtgtgcacac
     7621 gatcgttgaa cgctatgtcc gcgactggga actccaaccc gccgaaacct gcatctacgc
     7681 gtgcggccac ccgcaaatga tcgaggactt gcgcggccgc tacgccggca cggggttcga
     7741 gttcgaggag gagcgcttct ggaagcccca ctagagagtg tggcgtgccg cacacccgga
     7801 gcccgcgcct gcgagctaca gtcgcaacat gtccgtgatc ggaagcatcc aatgaagcga
     7861 cattggcaca ggaatttgcg ctgggcacct ctgtcgctca cggcgctgct cgcaacggcg
     7921 tgcggcgggc atggcggcga cgacgccgca ggcgctgggc cgccgggcag cgtcagtagc
     7981 ggttgggtcg ccggatcgtt cctgccggct gctagcttcg ccggccgttg cgtcaatccg
     8041 cgcgccggca cgaatccctc gggcggcatc gacactccgg gcacgacgct cgaccagaac
     8101 aact
//
LOCUS       DATLEE010000276         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6117345, whole genome shotgun sequence.
ACCESSION   DATLEE010000276 DATLEE010000000
VERSION     DATLEE010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6117345"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2032)
                     /locus_tag="VM692_05805"
     CDS             complement(<1..>2032)
                     /locus_tag="VM692_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUQ51715.1"
                     /translation="EPGIGKTTLARSLAQRAHADGATVLFGRCDEDIGVPYQPFVEAL
                     GGYISAATEEALRIIDQRSLSELSRLLPEVISTVPGVVEPQATDADAERYLFFGAVAS
                     MLTDMAAAAAVVLVLDDLHWADKSTVLLLRHLVGTLGPAQVLIVCTYRESDISAVHPL
                     SEGLSALRREPSVERVAVEGLDDGGVYALVEAMAGHELPSDGAELAQAVRRETGGNPF
                     FAAEVLRHLAETGAVVQQEGRWVAGADLFTIGLPESVREVVGQRVRRLGEDVHRLLTV
                     ASVIGRDVDLSLLARVAGVDDDVALAALESAVERLIVTEVEDRPGRFTFTHALFQRTL
                     YDELSGSRRARLHRQIAEMLEAECGDEPGDRIVELAHHWIAAMRPAEGGKAVRYARRA
                     GEHALATLAPHEAIRWFRQSLELVDSEAVVDPFLRLDCLIGLGDAQRQAGDPSYRETL
                     LDAAATAVGLGATDRLVAAALANQRGMVSSVGAVDGERIAMLEAALDAIGNDDTPERA
                     RLLATLAAELPFNGDHERVCAIATDAEAVARRLGDDATLVAVLNLTALPLWVPDNFTR
                     SVAASQEALTLADRLGDPVAAFWAAMNRVYAMASSVDRPAAEAALDKASALAERIGQP
                     YLIWQARYVRSLLVLLSGDADEAERIAAEALDVAISSGQPDAMVVFGANLLGV"
BASE COUNT          321 a          712 c          698 g          301 t
ORIGIN      
        1 acgccgagaa ggttggcgcc gaacaccacc atcgcatcgg gctgtcccga agagatggcg
       61 acatcgagcg cttcggcggc gatgcgttcg gcttcgtcgg cgtcaccgct gagcaacacg
      121 agcaagctgc ggacgtaacg cgcctgccag atgagatacg gctggccgat ccgctcggcc
      181 agcgccgagg ccttgtcgag cgccgcctcc gccgccgggc ggtcgacgct cgacgccatc
      241 gcgtacacgc ggttcatcgc cgcccagaac gcggccacgg gatctccgag ccgatccgcc
      301 agcgtgagcg cttcctgcga cgcagccacg ctgcgagtga agttgtcggg aacccacagc
      361 ggcaacgccg tgaggttgag cacggcgacg agggtggcgt cgtcgccaag ccgccgtgca
      421 acggcttcgg cgtcggttgc gatggcgcaa acgcgctcgt ggtcgccgtt gaacggcaac
      481 tccgcggcga gcgtcgccag gaggcgagcg cgttcgggag tgtcgtcgtt gccgatggcg
      541 tcgagcgcgg cttcgagcat ggcgatgcgc tcaccgtcga cggcgccgac gctgctcacc
      601 atgccgcgct ggttcgccaa tgccgccgcc acaagccggt cggttgcgcc gagcccgacg
      661 gcggttgcgg ccgcgtcgag caacgtctcg cggtagctcg ggtcgccggc ttgccgttgc
      721 gcgtcgccaa ggccgatcag gcagtcgagg cggaggaacg gatcgacgac cgcttcagag
      781 tccacgagct cgagcgactg gcggaaccac cggatcgctt cgtgcggcgc cagcgtggcg
      841 agcgcgtgtt cgccggcgcg gcgagcgtaa cgaacggcct tgccgccctc ggccggacgc
      901 atcgccgcga tccagtgatg cgcgagctcc acgatgcgat cgccgggctc gtcgccgcac
      961 tcggcctcca acatctctgc gatctgacgg tgcagtcgcg cgcggcggct gccggacagc
     1021 tcgtcgtaca gcgtgcgttg gaacagtgcg tgcgtgaacg tgaaccgacc cggacggtct
     1081 tcgacttcgg tgacgatcaa acgctcaact gcggactcca acgcagcaag cgctacgtcg
     1141 tcgtcgacgc cggcaacgcg cgcgagtagc gacaggtcga cgtcgcgtcc tatcaccgac
     1201 gcgaccgtca gcagtcggtg aacgtcttcg cccaaacgcc gaacgcgttg gccgacgact
     1261 tcgcgcacgc tctccggcag gccgatcgtg aagaggtccg cgccggcgac ccagcgacct
     1321 tcctgctgca ctaccgcgcc cgtctccgcg aggtggcgca acacctcggc ggcgaagaag
     1381 gggttgcctc ccgtctcgcg ccgcacggcc tgcgccagct cggcgccgtc gctcggcagc
     1441 tcgtgtccgg ccatcgcctc gacgagcgcg tacacgccgc cgtcgtcgag gccctcgacg
     1501 gccacgcgct cgacgctcgg ttcgcgccgc agcgcgctga ggccctccga caacgggtgg
     1561 acggcgctga tgtcgctctc gcggtacgtg cacacgatca gcacttgcgc cgggccgagc
     1621 gtgcccacga ggtggcgcag cagtaggacc gtcgacttgt ccgcccagtg cagatcgtcg
     1681 agcacgagca cgacggccgc cgctgcggcc atgtcggtca gcatcgacgc cactgcgccg
     1741 aagaagaggt agcgctcggc gtcggcgtcg gttgcctgcg gctccacgac accgggaacc
     1801 gtcgaaatca cttcgggcaa cagtcgcgac agctcgctca gggatcgctg gtcgatgatc
     1861 cgcagcgctt cttcggtggc agcgctgatg tatccgccga gcgcttcgac gaacggctgg
     1921 taaggaacgc cgatgtcttc gtcgcaacga ccgaacagaa ccgtcgctcc gtcggcgtgc
     1981 gcgcgctgcg cgagggagcg ggcgagcgtc gtcttaccga taccgggttc ac
//
LOCUS       DATLEE010000277         2324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4780801, whole genome shotgun sequence.
ACCESSION   DATLEE010000277 DATLEE010000000
VERSION     DATLEE010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4780801"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VM692_05810"
     CDS             <1..297
                     /locus_tag="VM692_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HUQ51716.1"
                     /translation="HPGALWSATYYVDDGGVGADASLGGEFEIQDPRGVAPVMYAPYL
                     TFPGPDGASLGEAQRLTPQAGMFVVFPSWLSHGVRPYRGTRERISIAINFSLGG"
     gene            302..1714
                     /locus_tag="VM692_05815"
     CDS             302..1714
                     /locus_tag="VM692_05815"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HUQ51717.1"
                     /translation="MSVELFFSVPLLVEDIDTAARDAIHAKVTAYLKSERAKRDVGPA
                     AEESVQTSYYKPEAQVLIDAELHELERHVLASAAKFLERLRLPPRRLEIERSWINVFE
                     PGAQEAQHSHDGSLLSCSYYVEAPKDCGCIVMPDPIGARRSYREFTKTAGNDLLTRRE
                     IAVEPQPGRLVMFESWLPHYVQCNKSEHVRISIAMNLREAATQAPSPPARAATPAGGA
                     AAAASASAPRARSADAEPFLFEQLFEVNPDLKLRLEPIQNAIPTIVIDDFLKHPEEAR
                     EVVGSAPAANWKHEKGGRNFIDYFDCRLRFPIRPPNAMIAAAQQVIRKVYALDTRPTE
                     ASVDVNWFRQIHEKRADFAVPHSDRTSAQRSLTCILYLNRHEECSGGTAFFRFKKSGS
                     LVFDESYARQVQADARLAETGLDYWSGAADDWWELVGTVDMVPGRLLIFPSEYFHAAY
                     HPQNSFYDFPRLTLAFWMVT"
     gene            1728..>2324
                     /locus_tag="VM692_05820"
     CDS             1728..>2324
                     /locus_tag="VM692_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HUQ51718.1"
                     /translation="MNPTLWQASPIVVVGMHRSGTRLLVELLARLGVFMGADRQGDAE
                     SVAFMLLNEQLLHQCGAFWSEPMSAHFMLAEPNAAAQLAEGARAALAASFEQYAGASG
                     WHGDAGTQLPAFGWKDPRNTFTLPVWKQVFPNLRTIHIVRHGVDVAASLARRHAEALR
                     AATGEAVPSALAVIRDHAFGILSSRRGWTLAEALTMWEQ"
BASE COUNT          376 a          776 c          768 g          404 t
ORIGIN      
        1 caccccggtg cgctgtggtc cgcgacctat tacgtcgacg acggcggcgt cggcgccgac
       61 gcgtcgctcg gcggcgagtt cgagatccag gacccgcgtg gcgtggcccc ggtcatgtac
      121 gcgccgtatt tgacctttcc cggccccgac ggcgcgtcac tcggcgaggc acagcggctg
      181 accccgcagg ccggaatgtt cgtcgtgttt ccgagctggc tgtcgcacgg cgtccgcccc
      241 taccgcggga cgcgcgagcg catctccatt gccatcaact tcagcctagg cgggtagcgt
      301 cgtgtccgtc gagctcttct tctccgtgcc gctactcgtc gaggacatcg acacggcggc
      361 gcgcgacgcc atccacgcca aggtcactgc gtatctcaaa tccgagcgcg cgaagcgcga
      421 cgtcgggccc gcggccgagg agtccgtgca gacgtcttac tacaagcccg aggcgcaggt
      481 tttgatcgac gccgagctgc acgagctcga gcgccacgtc ctcgcatcgg ccgcgaagtt
      541 tctcgagaga ctgcggctgc cgccgcggcg gctcgagatc gaacgctcgt ggatcaacgt
      601 cttcgaaccg ggcgctcaag aggcccagca ctcgcacgac ggcagtttgc tgtcctgcag
      661 ctactacgtc gaagcgccca aggattgcgg gtgcatcgtg atgccggatc cgatcggcgc
      721 gcggcgcagc tatcgcgagt tcacgaagac ggccggcaac gatctgctga cgcgacgcga
      781 gatcgccgtc gagccgcagc cgggccgcct cgtgatgttc gagtcgtggt tgccgcatta
      841 cgtccaatgc aacaagagcg agcacgtcag gatttcgatc gcgatgaatc tgcgtgaggc
      901 tgcaacgcag gcgccatcgc cgccggctcg cgcggcgact ccggccggcg gcgccgctgc
      961 ggcggcgagc gcgtcggctc ctcgagcgcg atcggccgac gccgagccgt tcctgttcga
     1021 gcagctgttc gaggtcaatc cggacctgaa gctgcgcctc gagccaattc agaacgcgat
     1081 cccgacgatc gtcatcgacg atttcctgaa gcaccccgag gaggccagag aggtcgtcgg
     1141 cagtgcgccg gccgcgaact ggaagcacga gaagggtggg cgcaacttca tcgattattt
     1201 cgattgccgc ttgcgcttcc cgatccggcc gccgaacgcc atgattgccg ccgcgcagca
     1261 agtcattcgc aaagtttatg cgctcgacac gcggcccact gaagccagcg tcgacgtcaa
     1321 ctggtttaga cagatccacg agaaacgcgc ggactttgca gtcccgcaca gcgacaggac
     1381 gagcgcgcag cgctctctga cgtgcattct ctacttgaat cggcacgagg agtgctccgg
     1441 cgggacggcc ttcttccgct tcaagaagtc cggctcgctc gtgttcgatg aaagttacgc
     1501 tcgccaggtc caggcggatg cgcggctcgc cgaaaccggg ctcgactact ggtccggtgc
     1561 ggccgacgac tggtgggagc tcgtcggaac ggtcgacatg gtgccaggac gcctactgat
     1621 tttcccctcc gagtacttcc atgccgccta tcacccgcag aactcgttct acgactttcc
     1681 gcggttgacg ctggccttct ggatggtcac ctgacgagct cgcgcgcatg aacccaacgc
     1741 tgtggcaggc tagcccgatc gtcgtggtcg gcatgcatcg ctcgggcact cggctgctcg
     1801 tcgagctcct cgccaggctg ggcgtcttca tgggagccga tcggcaaggc gacgccgagt
     1861 ccgtcgcgtt catgttgctc aacgagcagc tgctgcatca gtgcggagcg ttctggtccg
     1921 agccgatgtc ggcgcatttc atgctggcgg agccgaacgc ggcggcgcag cttgccgaag
     1981 gcgcgcgcgc ggcgctcgcc gcgagcttcg agcagtacgc gggagcgtcg ggctggcacg
     2041 gcgatgcggg cacacagctg ccggccttcg gctggaagga ccctcgcaac acgttcacgc
     2101 tgcccgtctg gaagcaggtg ttcccgaacc tgcgcacgat tcacatcgtc cgacacggcg
     2161 tggacgtcgc ggcgagcctc gcgcgccggc atgccgaggc attgcgtgcc gcgaccggcg
     2221 aggcggttcc gtcggcgttg gcggtgatcc gcgatcacgc gttcggcatc ctgtcgtcgc
     2281 gccgcggctg gacgcttgcc gaggcgttga cgatgtggga gcaa
//
LOCUS       DATLEE010000278         4404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9253684, whole genome shotgun sequence.
ACCESSION   DATLEE010000278 DATLEE010000000
VERSION     DATLEE010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9253684"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VM692_05825"
     CDS             <1..841
                     /locus_tag="VM692_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HUQ51719.1"
                     /translation="WLASALVMAVVVATSVDAQRSPPAPATPRIYIFENGSIRGLDPA
                     LFNFKREDLKEVDFVNASYLVVHPRGTLMFDTGAVADSHFKGDGKPVEEGIMTATKPL
                     LPQLAAAGYSPRDITYFALSHYHSDHTANANEFAGSTWIVQKAERDFMFGDTPQGIIQ
                     PATYAALRNAKTKVLDNEDFDVFGDGSVVIMSTPGHTPGHQVLAVKLANRGTVVLGGD
                     LYHYPEERTTGRIPTFEFNPEQSKVSRARIEQYLKDNKATLWIEHDLATHAALPKSPQ
                     YVD"
     gene            847..1572
                     /locus_tag="VM692_05830"
     CDS             847..1572
                     /locus_tag="VM692_05830"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ51720.1"
                     /translation="MATVEEHYDRLLADVYSWMYGGWDAALARYTEFFAARGILPRKS
                     ARVVDLGAGCGFQAIPLARVGFDVTAFDLDRKLLAELERHAGSLKIETVCADLVTFRS
                     HAHEPLELVVCMVDTLLHLDSEEVVQGLFRDVFAALEPDGTFIATFRDFSVAAEDLDR
                     FISVRNDERMVFTCFLEFEPEKVKVHDLVYRRVDGRWDFAKSYYRKLRLSTAWVVSTL
                     RAVGFERVDTGLDRGLVVVTCTK"
     gene            1649..2446
                     /locus_tag="VM692_05835"
     CDS             1649..2446
                     /locus_tag="VM692_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HUQ51721.1"
                     /translation="MWSLLVAAWMAGQGQPLQMSYPNEPGLAGITVRWSGHDVPFVRS
                     GDRWLGIVGVDLDSKPGDHAVDVTFKYDDGRSRVVREPVAVTAGQYPTTELEVEERFV
                     ELSPEDQARADREAAETSAIYDTFTPERLWTAPFTIPVRGAKDGRNFGHRRVFNGQPR
                     APHSGADLRATTGTPIYAANRGRVVLAKNLFFSGNAVFIDHGYGLFTTYLHLSKIDVA
                     VGDIVERGQELGLAGATGRVTGPHLHWGVRLVDARVDPFALVRLGAP"
     gene            2459..2638
                     /locus_tag="VM692_05840"
     CDS             2459..2638
                     /locus_tag="VM692_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HUQ51722.1"
                     /translation="MSNHPGRNDPCPCGSGKKFKHCHALKQDRLSLGTRIWFVLIGAM
                     LLFGAWLAVTTIDIG"
     gene            2845..3318
                     /gene="rimP"
                     /locus_tag="VM692_05845"
     CDS             2845..3318
                     /gene="rimP"
                     /locus_tag="VM692_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HUQ51723.1"
                     /translation="MGRTEHMGQAVDKEGLLAILEPAVTAMGFELADIDAYFGRRGLL
                     RLYIDRAGGVTVDDCQRVSEQVGALLDVEDPLPGSYVLEVSSPGFDRRLRTLAHFERF
                     GGEQVKVELKDAREGRRNFKGRLVGVEGSEVLLEVDGELTRLPFGDIAVARLAPP"
     gene            3337..>4404
                     /gene="nusA"
                     /locus_tag="VM692_05850"
     CDS             3337..>4404
                     /gene="nusA"
                     /locus_tag="VM692_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HUQ51724.1"
                     /translation="MSKEILLVVDAVSNEKGVEKSVIFEAIEAALASATRKRYGEDID
                     VRVAINRASGSYDTFQRWKVFADDSREIEEPARELRLEDALDIDPKAEIGGFVERPMQ
                     SVEFGRIAAQMAKQVIVQKVREAEREQVVEAYKHRAGELVSGIVKRVDRNGAYVDLGS
                     NAEGFVPREDLIPREPIKPQDRIKSLLREVRAEPRGPQLFLTRTSPKFLIELFKIEVP
                     EVGQGLIEILGAARDPGLRAKIAVRSHDSRIDPVGACVGMRGSRVQAVSNELAGERVD
                     IILYDENPAQFVINAMSPAEVLSIVVDEDTHTMDLAVEEEKLSQAIGRGGQNIRLASE
                     LTGWQLNVMTESAAEEKSDKEA"
BASE COUNT          783 a         1367 c         1486 g          768 t
ORIGIN      
        1 ctggctcgcg agcgcgctcg tcatggctgt tgtcgtcgcg acgagcgtcg acgcacaacg
       61 ctcgccgccc gcgccggcca cgccgcgcat ttacattttc gaaaacggct cgattcgcgg
      121 cctcgatccc gcgctgttca actttaagcg cgaggacctc aaggaagtcg acttcgtcaa
      181 cgcgtcgtat ctggtcgtgc acccgcgcgg gacgttgatg ttcgacacgg gcgccgtggc
      241 cgatagccac ttcaagggcg acggcaagcc cgtcgaagaa ggcatcatga cggcaacgaa
      301 accgttgctg ccgcagctcg cggcggcggg ctattcgccg cgcgacatca cctattttgc
      361 gttgtcgcac taccattcgg accacacggc gaatgccaac gagttcgcgg gatcgacttg
      421 gatcgtgcag aaagccgagc gcgatttcat gttcggcgac acccctcaag gcatcatcca
      481 accggcgacc tatgcggcgc tgcgcaatgc gaagacgaag gtcctcgaca acgaggactt
      541 cgacgtgttc ggcgacggca gcgtcgtcat catgtcgacc ccggggcaca cgccgggcca
      601 tcaagttctg gccgtgaagc tcgccaaccg cggcaccgtg gtgctcggcg gcgacctcta
      661 ccactatccc gaagaacgca cgacgggacg cattccgact ttcgagttca accccgagca
      721 atcgaaggtg tcgcgcgctc gcatcgagca gtacttgaag gacaacaaag cgacgctgtg
      781 gatcgagcac gacttggcga ctcacgccgc gctgccgaaa tcgccgcagt acgtcgacta
      841 gccgcgatgg ccaccgtcga ggagcactac gaccgtctgc tcgcggacgt ctactcatgg
      901 atgtacggcg gctgggatgc ggcgctcgcg cgttacacgg agttcttcgc ggcgcgcggc
      961 atcctgccgc ggaagtcggc gcgcgtcgtc gacctcgggg cgggctgcgg gttccaggca
     1021 attccgctgg cgcgcgtcgg cttcgatgtc acagcgttcg atctcgaccg gaagttgcta
     1081 gccgagctcg agcggcatgc cggcagcttg aagatcgaga ccgtttgcgc ggacctcgtt
     1141 acctttcgca gccacgcgca cgagccgctc gagctcgtcg tctgcatggt cgatacgctg
     1201 ctgcatctcg actccgagga agtcgtacaa ggcctgtttc gcgacgtgtt tgcggcgctc
     1261 gagccggacg gcacgttcat cgcgacgttc cgtgatttct cggtcgcggc cgaggacctc
     1321 gaccggttca tctccgtccg caacgacgag cgcatggtct tcacgtgctt cctcgagttc
     1381 gagcccgaga aggtcaaggt tcacgatctc gtgtaccggc gcgtcgatgg gcgctgggat
     1441 ttcgcgaaga gctactaccg caagttgcgc ctctcgacgg catgggtggt ctcgacgctg
     1501 cgggccgtcg gcttcgaaag agtcgacacc gggctcgacc gcgggctcgt cgtcgtgacg
     1561 tgcacgaagt agctgcgtcc ttgacccgca atgccggggc tcggatgatc gagcgcgagc
     1621 ttgggcgtag gtaggagtag cggcaccgat gtggtcgttg ctggtcgcgg catggatggc
     1681 ggggcagggt caacccctgc aaatgagcta cccgaacgaa ccggggctgg ccggcatcac
     1741 ggttcgctgg agcgggcacg acgtgccgtt cgtgagaagc ggcgaccgtt ggctcggcat
     1801 cgtcggcgtc gacttggact ccaagcccgg cgatcatgcc gtcgacgtca cgttcaagta
     1861 cgacgatggc cgttcgcgcg tcgtgcgcga gcccgtagcc gtcacggctg ggcaataccc
     1921 gacgaccgag cttgaggtcg aagagcgctt cgtcgagctc agccccgaag accaggctcg
     1981 cgcggaccgc gaggcggccg agacgagcgc catctacgac actttcacgc cagagcgcct
     2041 ctggacggca cccttcacga tccccgtgcg cggcgcgaag gacggccgca acttcggcca
     2101 tcgccgcgtc ttcaatggcc agccgcgcgc gccgcattcc ggcgcggacc tgcgagcgac
     2161 caccggcacg ccgatctacg ccgcgaaccg cggccgtgta gtgttggcca agaacctgtt
     2221 tttcagcggc aacgccgtgt tcatcgacca cgggtacggc ctgtttacga cctacctaca
     2281 cctgtcgaag atcgacgtcg ccgtgggcga catcgtggaa cgcggtcaag agcttggtct
     2341 ggccggggcg acgggcagag tcacgggccc gcatctgcac tggggcgtgc ggctcgtgga
     2401 tgcccgagtc gacccgttcg cgttggtccg gttgggagcg ccgtaacggt gtagcgacgt
     2461 gagtaaccac cccgggcgca acgatccgtg tccctgcggc agcggcaaga aattcaagca
     2521 ttgccacgcg cttaagcagg accgtctgtc gctcggcacc cggatctggt tcgtcctgat
     2581 aggggccatg ctgctgttcg gagcctggct ggccgtgacg acaatcgaca taggctgaaa
     2641 gccgcctacc gcctgacccg ccttttctgc tagcattgcc gcgttttccg gagcaacggc
     2701 tctgaaggca agaagccccg tatggggctt tttgtttggt ccggctcatc gcacaggcga
     2761 tcgagcgctc gcaagggaga aggggactcg aggggaatgg gccgttacgg cccatttttg
     2821 ttggtgccgg cgggctggct ctcgttggga cgcacggagc atatgggcca ggcggtcgac
     2881 aaagaaggtt tgttagcgat tctcgagccg gccgtgacgg ccatggggtt cgagctcgcc
     2941 gacatcgatg cgtacttcgg gcggcgcggt ctgctgcggt tgtacatcga ccgcgcaggc
     3001 ggcgtgacgg tcgacgactg ccagcgagtc agcgagcagg tcggggcgct gctcgacgtc
     3061 gaggaccctt tgccgggcag ttacgtgctc gaggtgtcgt cgccgggatt cgatcgtagg
     3121 ttgcgcacgc ttgcgcactt cgagcgcttc ggcggcgagc aggtgaaggt cgagttgaag
     3181 gatgcgcgcg agggtcgacg taattttaaa ggtcggctcg taggtgtgga gggcagcgag
     3241 gtgctgctcg aagtggacgg cgaacttacg cgtttgcctt ttggcgatat cgcggtggcg
     3301 cgattagcgc cgccgtgacg caaagtcggt gacgagatga gcaaagaaat tttgttggtg
     3361 gtcgacgcgg tctcgaatga gaagggcgtc gagaagtcgg tgatcttcga agccatcgag
     3421 gcggccctcg cgtccgccac gaggaagcgc tacggggagg acatcgacgt ccgcgtcgcg
     3481 atcaatcggg cgagcggcag ctacgacacg tttcagcgtt ggaaggtgtt cgccgacgat
     3541 tcgcgcgaga tcgaggagcc ggcgcgcgag ctacggctcg aggacgctct cgacatcgat
     3601 ccgaaggccg agatcggcgg cttcgtcgag cggccgatgc agtccgtcga gttcggccgc
     3661 atcgccgcgc agatggcgaa gcaagtcatc gtccagaagg tgcgcgaggc cgagcgcgag
     3721 caggtcgtcg aggcgtacaa acaccgcgca ggcgagctcg tcagcggcat cgtcaagcgc
     3781 gtcgaccgca acggcgccta tgtggatctc ggcagcaacg ctgagggttt cgtgccgcgc
     3841 gaggacctga tcccgcgcga gccgatcaag ccgcaagacc gcatcaagag cctgctgcgc
     3901 gaagtacgcg ccgagccgcg cggaccgcag ctgttcctga ctcgaacgtc gccgaaattt
     3961 ctgatcgagc tcttcaagat cgaagtgccc gaagtcggcc aaggcctcat cgagatcctc
     4021 ggcgctgcgc gcgatcccgg cttgcgggcc aagatcgccg ttcgtagcca cgacagccgc
     4081 atcgaccccg tcggcgcctg cgtcggcatg cgcggctcgc gtgtccaagc cgtctcgaac
     4141 gagctggccg gcgagcgcgt cgacatcatc ctctacgacg agaaccccgc gcagttcgtc
     4201 atcaacgcga tgtcgccggc cgaggtgctg tcgatcgtcg tcgacgagga cacgcacacg
     4261 atggatctcg ccgtcgagga agagaagctg tcgcaggcca tcggccgcgg cgggcagaac
     4321 atccggctcg caagcgagct gacgggctgg cagttgaacg tcatgacgga gtccgcggcc
     4381 gaggagaaga gcgacaagga agcg
//
LOCUS       DATLEE010000279         7867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4787655, whole genome shotgun sequence.
ACCESSION   DATLEE010000279 DATLEE010000000
VERSION     DATLEE010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4787655"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1516
                     /locus_tag="VM692_05855"
     CDS             167..1516
                     /locus_tag="VM692_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HUQ51725.1"
                     /translation="MKPLLEDAARRAAAYVASLQERSVAPALEAVRGLSALDVPLQAE
                     PRDAQRVLAELDELVSPATMAMAGPRFFGFVIGGSLPAALAANWLAGAWDQNAGLYSV
                     TPGVAHVEQVVLRWLNDLFGLPAGTTGALVTGATMANFSSLAAARHAVLQRAGWNVEA
                     DGLFGAPPVTVIVGEEVHITVLKALGMLGLGRNRVVRVPADSQGRMRASELPRISGPT
                     IVCVQAGNVNSGAFDPVAQVCDGVHEGGAWVHVDGAFGLWAKASPRYAHLVHGIERAD
                     SWSTDAHKWLNVPYDCGIALVRDPDALAAAMTIQAAYIPHGDAQRNPFDYTPEFSRRA
                     RGVDVWAALRSLGRRGLAAMIERDCAAAQRFAERLAAAGCEILNDVVLNQVLVSFGEP
                     DETQRVIAAVQADGTCWVGGTVWQGRTAMRISVCSWATTEADVDASVAAMLRAKGSS"
     gene            1513..1995
                     /gene="tadA"
                     /locus_tag="VM692_05860"
     CDS             1513..1995
                     /gene="tadA"
                     /locus_tag="VM692_05860"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HUQ51726.1"
                     /translation="MTPAEAAVDRARMLEALDCAREAESLGEVPVGAVIVDAAGAVIA
                     RAANAPIARHDPTAHAEILALRAAGRALGNYRLPKCVLYVTLEPCAMCVGALVHARVA
                     RIVYGAADPKTGACGSVFELAASAKLNHRIEVAGGVLAEESAALLTQFFAARRRRDAL
                     "
     gene            complement(1992..3365)
                     /gene="mltF"
                     /locus_tag="VM692_05865"
     CDS             complement(1992..3365)
                     /gene="mltF"
                     /locus_tag="VM692_05865"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HUQ51727.1"
                     /translation="MLAAIVLGAMLSTCSSPPSVLERILRSGELRVVTRNTPAAFYYG
                     ADEPRGIEYELARGYAEKLGVSLRIYIDDQVFPDLVSGKAQIGAASLTVADARRDSVA
                     FGPGYQQIEPLIIYRRGTPRPRKLGDLVGGRLEVVASSSHAALLEKLRSQEPLLTWIE
                     HTQRSSEELIRRVADGEIDYTIADSHLFELLRHFYPDLRVAFPLGPTDQIAWAIPKGD
                     EPLRESIAAYFAEIQATGELKQILDRYYFASSEFDYIGSRAFVRHVNTRLPRYRDFFL
                     EAERSTGIDWRLLAAIAYQESHWDPAAVSPTGVRGMMMLTEHTANMMEVGNRHDARAS
                     VLGGGHYYLRVRRKVPERIGEPDRTWLAVAAYNLGFGHLEDARILTQMQGADPDRWEE
                     VRDRLPLLSDKAWFSRVQRGFAPGQTAVAYVDNVRRYYEILMWLDSHETLTSQDYTPP
                     NLLGSGG"
     gene            3523..4752
                     /locus_tag="VM692_05870"
     CDS             3523..4752
                     /locus_tag="VM692_05870"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HUQ51728.1"
                     /translation="MPNSLVAAVVAASFALCTGAAAQPAASAEIAAIRAEMAGLMARL
                     ERLERGNAAAATSAAAAATPAAAAPADARVAATVPPNIRFAGDMRYRHEAINEDGVAE
                     RERQRIRARFGVTADIAKNVRVGLQLASGTDDPVSANQTLDTGFNRKQFGIDRAFFTW
                     AATEQLTFTGGKFANPFFRPGNHHLIYDNDLNPEGLALRYARGDWFANYAGLWVEERS
                     AADDSIMLGGQFGFRHMLDNGMRVTAGASYYDYLETQGRTPFWDAAAAGNRLAPGNVY
                     LNDFNMTELFGELNLKAGERPVTVFADYVVNSEAADADTGFALGASLGEVTRAGTWRI
                     GYAYQDLEADAVIGTFTDSDFAGGGTDGKGHVVEFNYGFRDRLVFGLRYFLNERGADA
                     GTEHDYNRLQADVVFNY"
     gene            4903..5355
                     /locus_tag="VM692_05875"
     CDS             4903..5355
                     /locus_tag="VM692_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51729.1"
                     /translation="MSREIMSTVPQLRPTVAKERFDSNGARKAAIALLTAIYKTGRHG
                     TMAEVLIALEVIDSNLEGRPHSMKSLSSKLGIPYTSVSRIVYSLTAEAQPGGVLKLVP
                     DSKDRRRKHIELDTDALKRMSSHVRALEKAMIDYYGSSVYKLKKDQPN"
     gene            5410..6183
                     /locus_tag="VM692_05880"
     CDS             5410..6183
                     /locus_tag="VM692_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HUQ51730.1"
                     /translation="MRRPSAKWLWLIPPFLFGMAYLDRVEGFVDGRDARRAPIGLAPN
                     PRTTPEPVVQVYAARAVRWRGYFGVHTWVAVKPRDADEFTIYEVNGWRLRRTGTAVSV
                     DHRPPDARWFGNRPELLADVRGDGVDDVITRIEAAVASYPYADTYRVWPGPNSNTFVA
                     HLLRAAPELRADLPANAIGKDYLERKFVARSPSGTGAQVNLFGVVGALAGIEEGLELS
                     VLGLTFGVDPLDLSLKVPMAGRLGWPRPRAPGVAAAEEN"
     gene            6185..7288
                     /locus_tag="VM692_05885"
     CDS             6185..7288
                     /locus_tag="VM692_05885"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HUQ51731.1"
                     /translation="MRTLLAIIAVVAVLLVGQARAEPHQFTFSWQFEDGDALRPRGGT
                     TRGAKLVLATEPSAAWRAVQEPGLSKFERDRRAILAMAGGYRTSFDFLETVGFTPGYT
                     PSRPYQSWGTEYIYVAEDSGTFIRLQHLMVMFVADRDGKVQGPFVQKHWRQDWRYEDT
                     DLHVFVGRNRWVHQERTADEARGRWSQAVFQVDDSPRYEALGEWEHYGTYSAWTSERT
                     ARPLPRRESSVRSDYQILEGTNRHTLTPTGWVQEEENLKLVLDAQGQPAAGTPYLARE
                     VGVARYEHVVDFDWSAGDRYWQRSGGFWRDVRAAWDAVYAVRDTFEYLEEVDGQEMFV
                     PLFEYADRLDGGEAYDAAAAAEIIRSTFDRHLR"
     gene            7367..>7867
                     /locus_tag="VM692_05890"
     CDS             7367..>7867
                     /locus_tag="VM692_05890"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HUQ51732.1"
                     /translation="MKHQAEVTDTVVLGSGYGDARALTAALVAKLERSLGEGRLELPS
                     LPEVAMKIRRALADDNVSVTEIARLLGSDPALAARTLRIANSAMFYRGSRPITSLNGA
                     VSQLGYKMVRNVAMSFAAQQVFIGYNSRQLRDLVSAVWRHSVHAAVISHMLARVRAKL
                     NADEAFL"
BASE COUNT         1287 a         2584 c         2708 g         1288 t
ORIGIN      
        1 gcaggacttg cttcaccacg aggaagccca tgaggtcgaa aaggtacttc tcttccgtgt
       61 tcatctattg gatctcctat ccgagcggcc tgcggtcaac atagccggtc ggacgcccgc
      121 ggggctagag tgacgaccgg atccattcac cgctgagagg ctgaagatga aaccgctgct
      181 cgaggacgcc gcgcgccgcg ccgccgcgta cgtggcgagc ctgcaggagc gcagcgtcgc
      241 gccggcgctc gaggccgtcc gcggcttgtc ggcgctcgac gtgccgctgc aggccgagcc
      301 gcgcgatgcg cagcgcgtgc tggccgagct cgacgagctc gtgagcccgg caacgatggc
      361 gatggcgggc ccgcggtttt tcggtttcgt catcggcggc tcgttgccgg ccgcgctcgc
      421 ggcgaattgg ctcgccggcg cgtgggatca gaacgccggc ctctactccg tgacgcccgg
      481 agtcgcgcac gtcgagcaag tcgtgctgcg ctggctcaac gatttgttcg ggctgccggc
      541 cggaacgacg ggcgctctcg tgacgggcgc gacgatggcg aacttcagct cgctcgcagc
      601 cgcgcggcat gccgtgttgc agcgcgccgg ctggaacgtc gaagccgacg gactgttcgg
      661 cgctcctccc gtgactgtga tcgtcggcga ggaagtgcac atcaccgtcc tgaaagcgct
      721 cggcatgctc ggcctcgggc gcaatcgcgt cgtgcgcgtg cccgccgatt cgcaagggcg
      781 catgcgcgcg agcgagctgc cgcgtatcag cgggccgacg atcgtctgcg tgcaggcagg
      841 caacgtgaac tcgggcgcgt tcgatcccgt cgcgcaagtc tgcgatggcg tgcacgaggg
      901 cggcgcctgg gtgcacgtcg acggcgcatt cggtctctgg gcgaaagcgt cgccacgtta
      961 cgcgcatctc gtgcacggca tcgaacgcgc cgactcttgg tccacggacg cgcacaagtg
     1021 gctcaacgtg ccctacgact gcggcatcgc gctcgtgcgc gacccggacg cgctcgccgc
     1081 ggcgatgacg attcaggccg cgtacattcc gcacggcgac gcccagcgca atccgttcga
     1141 ctacacgccg gagttctcgc gccgggcccg cggcgtggac gtgtgggcgg cgttgcgctc
     1201 gctgggacgg cgcgggttgg cggccatgat cgagcgcgat tgcgcggccg cgcagcgctt
     1261 cgcggaacgg ctcgcggcgg ccggctgcga aatcctcaac gacgtcgtct tgaatcaggt
     1321 gctcgtctcg ttcggcgagc cggacgaaac tcagcgcgtg attgcggccg tgcaggccga
     1381 cggcacgtgc tgggtgggcg gcacggtatg gcaagggcga acggcgatgc gcatcagcgt
     1441 ctgctcgtgg gcaacgaccg aggccgacgt cgacgcgagc gtcgcagcga tgctacgcgc
     1501 caaagggtcg tcatgacacc ggccgaagcg gcggtcgatc gagctcggat gctcgaggcg
     1561 ctcgactgcg cgcgcgaagc cgagtcgctc ggcgaggtgc ccgtcggcgc cgtcatcgtc
     1621 gatgccgccg gcgccgtgat tgcgcgtgcg gcgaatgcgc cgatcgcgcg gcacgatccg
     1681 acggcgcacg cggagatctt ggcgctgcgt gccgcggggc gcgcgctcgg caactatcgc
     1741 ttaccgaaat gcgtgctcta cgtgacgctc gagccgtgcg cgatgtgtgt cggcgcgctc
     1801 gtgcacgcgc gcgtcgcccg catcgtctac ggcgcggccg atccgaagac gggcgcgtgc
     1861 ggctcggtgt tcgagctcgc ggccagcgcg aaactgaatc atcgaatcga agtggcaggc
     1921 ggcgtgttgg cggaggagag cgcggcgctg ctgacgcagt tcttcgcggc gcggcgcagg
     1981 cgcgacgcgc tctagccgcc ggagcccagc aggttcggcg gcgtgtagtc ctgcgacgtc
     2041 agagtctcgt ggctgtcgag ccacatcagg atctcgtagt agcggcgcac gttgtcgacg
     2101 taggcgacgg ccgtttgacc gggcgcgaac ccgcgctgca cgcggctgaa ccacgccttg
     2161 tcgctgagca gcggtaaacg gtcccgcact tcttcccagc gatcgggatc ggctccttgc
     2221 atctgcgtga ggatgcgcgc gtcttcgaga tggccgaagc ccaagttgta ggccgcgact
     2281 gcgagccacg tgcgatccgg ctcgccgatg cgctcgggca ctttgcgccg cacgcgcaga
     2341 tagtaatgac cgccgccgag cacgctcgcg cgcgcgtcgt gacggttgcc gacttccatc
     2401 atgttcgccg tgtgctcggt cagcatcatc atgccgcgca cgcccgtcgg cgagaccgcg
     2461 gcgggatccc aatgcgattc ttggtacgcg atcgcagcga gcagccgcca atcgatgccc
     2521 gtcgagcgct ccgcctcgag gaagaagtcg cggtagcgcg ggagccgcgt gttgacgtgg
     2581 cgcacgaacg cgcgcgagcc gatgtaatcg aactcgctcg acgcgaagta gtaacgatcg
     2641 aggatttgct tgagctcgcc cgtcgcttgg atttcggcga aatacgccgc gatgctttcg
     2701 cgcagcggct cgtcgccctt cggtatggcc caggcgatct ggtccgtcgg cccgagcggg
     2761 aaggcgacgc gcaagtcggg atagaagtgg cgcagcaact cgaacagatg cgagtccgcg
     2821 atcgtgtaat cgatttcgcc gtcggcgacg cgccgaatga gctcctcgct ggagcgctgc
     2881 gtgtgctcga tccacgtcaa taacggctct tgcgaccgca gcttttcgag cagcgccgca
     2941 tgcgacgagc ttgcgacaac ctcgagccgg ccgccgacga ggtcgccgag cttgcgaggc
     3001 cgcggcgtgc cgcggcggta gatgatcaac ggctcgatct gctgatagcc cgggccgaac
     3061 gcgacgctgt cgcggcgcgc gtcggcgacc gtgaggctgg ccgcgccgat ctgggccttg
     3121 ccggacacaa gatcggggaa gacctggtcg tcgatataga tacggaggct gacgccgagc
     3181 ttttcggcat agccgcgcgc gagctcgtac tcgatgccgc gcggctcgtc ggcgccgtaa
     3241 tagaaagccg ccggcgtatt gcgcgtcacg acccgcagct ccccggaacg caggatgcgc
     3301 tcgagaaccg agggtggaga cgagcaggtg ctgagcatcg cccccagcac gatcgcggcc
     3361 agcattcgca acagcaaagc tccccgagtc gagacaacga ttatacggtg atcggcgctt
     3421 ccgttcggcc gacccctgcc gacgttctgt aacacgactg taatgcgacc gtagcattat
     3481 gcccgccggt tccgcaccac atcgcaatct ggagcaaaat caatgccaaa cagcctcgtt
     3541 gcggcggttg ttgcagcctc gttcgcactc tgcaccggcg ccgcggcaca gccggcagcc
     3601 agcgccgaaa tcgcggcgat tcgcgccgaa atggccggcc tcatggcgcg tctcgaacgc
     3661 ctcgagcggg gcaacgctgc ggcagcgaca tcggccgcgg ctgccgcgac acccgccgcg
     3721 gcagcgccgg ccgatgcgcg cgtggccgcc accgtgccgc cgaacatccg cttcgccggc
     3781 gacatgcgct accgccacga ggcgatcaac gaggacggcg tggccgagcg tgagcgccag
     3841 cgcatccgcg cgcggttcgg cgtcacggcc gacatcgcca agaacgttcg cgtcgggctg
     3901 cagctcgcat ccggcaccga cgatcccgtc tcggcgaacc agacgctcga cacggggttc
     3961 aaccgcaagc agttcggcat cgaccgcgcg ttcttcacgt gggccgcgac cgagcagctg
     4021 acgttcacgg gcggcaagtt cgcgaatccg ttcttccggc ccggcaatca tcacctgatc
     4081 tacgacaacg acttgaaccc cgaaggtctg gcgctgcggt acgcgcgcgg ggactggttc
     4141 gcgaattacg cgggtctctg ggtcgaggag cgcagcgcgg ccgacgactc gatcatgctc
     4201 ggcgggcaat tcgggttcag gcacatgctc gacaacggca tgcgcgtgac cgccggcgcg
     4261 agttactacg actacctcga gacgcagggg cgcacgccgt tctgggatgc agccgccgcg
     4321 ggcaatcgac tcgcgcccgg caacgtctac ttgaacgact tcaacatgac agagctcttc
     4381 ggcgagctga acctgaaagc cggcgagcgg cccgtcacgg tgttcgccga ctacgtcgtc
     4441 aacagcgagg cggccgacgc ggacacgggc ttcgcgctcg gcgcgagcct cggcgaagtc
     4501 acgcgcgccg ggacgtggcg catcggctac gcgtatcagg atctcgaggc cgacgccgtc
     4561 atcggcacgt tcacggactc cgatttcgcg ggcggcggga cggacggcaa aggccacgtc
     4621 gtcgagttca actatggctt ccgcgatcgc ctcgtgttcg ggctgcgcta cttcttgaac
     4681 gagcgcggtg cggacgccgg caccgagcac gactacaaca ggctccaagc ggacgtcgtg
     4741 ttcaattatt gatcatccgc actcgccgcg cggccgcgcg gcgagtgttt tttccactcg
     4801 cgtgattcgt gcccccgtac cgtacagtgc ggtgttgaga cgctcccgaa cgcatgaata
     4861 cactcgcggc gccaagcgct gatgcgctga tccggagtgg ctatgagcag ggagatcatg
     4921 tcgacagtac cgcaactgcg accgaccgtc gcgaaagaac gcttcgattc caacggcgcg
     4981 cgcaaagcgg cgatcgcttt gctgacggcg atatacaaaa ccgggcgaca cggcacgatg
     5041 gccgaggtgt tgatcgcgct cgaggtcatc gactcgaatc tcgagggtag gccgcattcg
     5101 atgaagtcgt tgtcgagcaa gctcggcatt ccgtatacgt cggtgtcgcg aatcgtgtac
     5161 tcgctgactg cagaggcgca gcccggcggc gtgctgaagc tcgtgcccga cagcaaggat
     5221 cgccgccgca agcacatcga gctcgatact gatgcgttga agcgcatgag ctcgcacgtg
     5281 cgtgcgctcg agaaggcgat gatcgattac tacggcagct cggtttacaa gctgaagaaa
     5341 gatcagccga attgacctcg gtgcggcggg cgcttgcccg cctcgtgtgc ccggtctatg
     5401 ttggctcgga tgcgacgccc gagcgccaaa tggctttggt tgattccgcc gttcctgttc
     5461 gggatggcgt acctcgatcg cgtcgagggc ttcgtcgacg ggcgtgacgc gcgccgcgcg
     5521 ccgatagggc tcgcgccgaa tccgcgcacg acaccggagc ccgtcgtgca ggtctacgcg
     5581 gctcgcgccg tccgctggcg cggctacttc ggcgttcaca cgtgggtcgc cgtcaagcca
     5641 cgcgacgccg acgagttcac gatctacgag gtcaacggct ggcgactgcg ccgcacgggg
     5701 acggccgtca gcgtcgatca tcggccgccc gacgcccgtt ggttcggcaa ccggccggag
     5761 ttgctcgccg acgttcgcgg cgacggcgtc gacgacgtca tcacgcgcat cgaagccgcc
     5821 gtggcgtcgt atccgtacgc ggatacctat cgcgtctggc ccggaccgaa ttcgaacacg
     5881 ttcgttgcac atctcctgcg cgcggcgccc gagttgcgcg ccgatttgcc ggcgaacgcg
     5941 atcggcaaag attatctcga gcgtaagttc gtcgcccggt cgccgagcgg cacgggtgcg
     6001 caggtcaacc tgttcggagt agtcggcgcg ctggccggca tagaggaagg cctcgagctc
     6061 agcgtcctcg gattgacctt cggcgtcgac cctctcgatc tgtcgttgaa ggtgccgatg
     6121 gcgggcaggc tcggttggcc gcgcccgcga gcgccgggcg tcgccgcggc ggaagagaac
     6181 tagcatgcgc acgttgctcg cgatcattgc cgtcgtcgcc gttctgctcg tcggtcaagc
     6241 gcgagccgag ccgcaccagt tcactttcag ttggcaattc gaggacggcg acgcgctgcg
     6301 accgcgcggt ggcacgaccc gcggcgcgaa gcttgtgctc gcgaccgagc cgagcgccgc
     6361 gtggcgggcg gtgcaagagc ccgggctctc gaagttcgag cgcgaccggc gcgcgattct
     6421 agcgatggcc ggcggctatc gcacgagctt cgatttcttg gagaccgtcg gcttcacgcc
     6481 gggctacacg ccgtcgcggc cgtatcagtc gtggggcacc gaatacatct acgtcgccga
     6541 ggacagcggc acgttcatcc gtttgcagca cctcatggtc atgttcgtcg cggatcgcga
     6601 cggcaaagtg caggggccgt tcgtgcagaa gcactggcgc caagattggc gttacgagga
     6661 cacggatctg catgtgttcg ttggccgcaa tcgctgggtg catcaagagc gcacggcaga
     6721 cgaagcgcgc ggccgttggt cgcaagccgt gttccaagtc gacgattcgc cgcgttacga
     6781 ggcgctcggc gagtgggagc actacggtac gtactcggcc tggacgagcg agcgcacggc
     6841 gcggccgctg ccgcggcgcg aaagcagcgt ccgcagcgac tatcagatcc tcgaaggcac
     6901 gaatcggcac acgctgacgc cgacgggttg ggtgcaggag gaggagaacc tcaagctcgt
     6961 cctcgacgcg caaggtcagc cggccgcggg cacaccgtat ctcgcgcgcg aggtcggcgt
     7021 cgcgcgctac gagcacgtcg tcgatttcga ctggtcggcc ggcgaccgct attggcaacg
     7081 cagcggcggg ttttggcgcg acgtgcgcgc cgcgtgggat gccgtctacg ccgtgcgcga
     7141 caccttcgag tatctcgaag aggtcgacgg tcaagagatg ttcgtgccgc tgttcgaata
     7201 tgccgaccgg ctcgacggcg gcgaggctta tgacgcggcc gccgcggccg aaatcatccg
     7261 cagcactttc gaccggcact tgcgctgagc ggccggcgcc ccttccggga ctaccgacag
     7321 cgtcacgggg ctaaagacct gcgcgccttc ggccgataca gcggccgtga agcaccaggc
     7381 ggaagtcacg gatacggtcg ttttgggcag cggctatggc gacgcgcgcg ccttgaccgc
     7441 ggcgctcgtc gcgaagctcg agcggtcgct cggcgaaggg aggctcgagc tgccgtcgtt
     7501 acccgaggtc gcgatgaaga tccgccgcgc gttggccgac gacaacgtgt ccgtcaccga
     7561 aatcgcgcgc ttgctcggct cggaccctgc gctcgcggcg cgcacgctgc gcatcgcgaa
     7621 cagcgccatg ttctatcgcg gcagccggcc gattacgagc ttgaacggcg ccgtctcgca
     7681 gctcggctac aagatggtgc gcaacgtcgc catgtcgttc gccgcgcagc aggtcttcat
     7741 cggttacaac agccgccagc tgcgcgacct cgtcagcgcc gtgtggcgcc acagcgtgca
     7801 tgccgcggtg atctcgcaca tgctggcgcg cgtgcgagca aagctgaacg ccgacgaagc
     7861 gttcctc
//
LOCUS       DATLEE010000280         3788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2557744, whole genome shotgun sequence.
ACCESSION   DATLEE010000280 DATLEE010000000
VERSION     DATLEE010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557744"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VM692_05895"
     CDS             complement(<1..262)
                     /locus_tag="VM692_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HUQ51733.1"
                     /translation="MAESIQAIANALFMPYVVAVLLGTGLFLTIRLRFVQLRRFGEAV
                     RAMLARPDAGAGGVLTPFQAFMTALGASVGTGNIAGVATAIVS"
     gene            complement(300..1928)
                     /locus_tag="VM692_05900"
     CDS             complement(300..1928)
                     /locus_tag="VM692_05900"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HUQ51734.1"
                     /translation="MRRVPMNWLSIPAILLTASTALAQTPESPSGVTAADIEAGLANP
                     TRWVTFSGDYSGARHSPLKQITPRNVARLKAQWTFQSGTYARGRGFETTPLAIDGVLY
                     ATGANGYAWAIDARTGRPFWEYRRTLPDNLTYGASAPVNRGFGTLGERLFLVSQDAHL
                     VALDRRSGVVLWDVELADYRIGYAATLAPLVVDDKVIVGISGGEYPTRGFVDAYDPAT
                     GARIWRLYTVPAPGEPGSDTWPGVQSAARGGGGTWMTGSYDPNLGLLYWGTGNPNPTF
                     YGVDRQGDNLYTDSLLAIEAATGKLRWHYQFTPHDTHDWDSNHVPVQATLPWQGGERR
                     VVMVANRNGFFYVIDRVTGELLLGKPYTAPTWAREIGPDGRPIVLNDDGSGGCLPDAW
                     GSTNFNPPSYDPKLRLFFVSARETCATFHGEQPQFVPGQNTAGGVVWADRDKAFGALR
                     ALDAMTGEMKWEFRFPQSNLAGVMSTASGLVFTGDQEGNFIAFDSVTGEELWHYQTGT
                     SIWGAAPMTYMLDGRQQVLIPSGSVLFSFALPQN"
     gene            complement(1940..2728)
                     /locus_tag="VM692_05905"
     CDS             complement(1940..2728)
                     /locus_tag="VM692_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUQ51735.1"
                     /translation="MTRRVAKRVVSTLWLSLCAAALSASAEQRNPFEGDEVAIRAGAG
                     LFAARCALCHGPDAKGQLGPDLTRRWARGETDESAFLVIRNGVPGSSMPPNAAPDNEI
                     WAIVAHLRSISVMPPLQSTGDASRGRAVFAADCAGCHQVRGEGGALGPDLTSIGALRS
                     RAALTAAVRDPSASVALGFRGVSVETRAGERMEGVVKSEDAFSLQVVTLNGELRAFRK
                     SELRALERSTESLMPVFDASRLNDAAFEDLLAYLGTLRGAQANP"
     gene            complement(2725..3663)
                     /locus_tag="VM692_05910"
     CDS             complement(2725..3663)
                     /locus_tag="VM692_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUQ51736.1"
                     /translation="MQQRRLGGSSLSVTPLVLGGNVFGWTADEPTSFAILDAFVAHGG
                     NAIDTADGYSYWVPGHQGGESETVIGRWLKKRGRRDDVLVCTKVGWWEKRKGLGRSNI
                     IEGCEDSLRRLGLESIDLFWLHRDDEQTPADEYLGALDTLVKAGKVRAVGASNFGVAR
                     FEAALAESERSGMARFAAQQPEYSLLNRAIEKDLMPLCQREGVAITPYYPLASGFLTG
                     KYRTSADKTKSVRGGGAVKNLEGKGKPVLAALDAVAARHGASCAQVALAWVMAKPAVA
                     APIASATSVAQVTELMGALDLKLAEADMAELDQASA"
BASE COUNT          620 a         1328 c         1237 g          603 t
ORIGIN      
        1 cggaaacgat cgccgttgca acgcccgcga tgttacccgt gccgacggac gcgccgagcg
       61 ccgtcatgaa cgcctgaaac ggcgtcagca cgccgccggc gccggcgtcc ggccgcgcga
      121 gcatcgcgcg cacggcctcg ccgaagcggc gcagctgcac gaagcgcagg cggatcgtca
      181 ggaacagccc ggtgccgagc agcacggcca cgacgtacgg catgaacaac gcgttggcga
      241 ttgcttgaat cgactcggcc atcacgcccc gcctgcggcg accgagtgcg cgacgcgcgt
      301 cagttctgcg gcagtgcgaa cgagaacagc accgagcccg atggaatcaa aacttgttgc
      361 cggccgtcga gcatgtaagt catcggcgcg gctccccaga tcgaagtgcc ggtctggtag
      421 tgccagagtt cctcaccggt caccgagtcg aacgcgatga agttgccttc ctggtcgccc
      481 gtaaacacga ggcccgaagc cgtgctcatg acgccggcca ggttcgactg tggaaagcgg
      541 aattcccact tcatctcgcc cgtcatggcg tcgagagcgc gcaacgcacc gaacgccttg
      601 tcgcgatcgg cccagacgac gccgccggcc gtgttttgcc ccggcacgaa ttgcggctgc
      661 tcgccgtgaa acgtcgcgca ggtctcgcgc gcactgacga aaaagagtcg gagcttagga
      721 tcgtacgacg gcggattgaa gttcgtgctg ccccacgcgt ccggtaagca accgccgcta
      781 ccgtcgtcgt tcagcacgat gggccggccg tcggggccga tctcgcgcgc ccacgtcggt
      841 gccgtatagg gcttgccgag caagagctcg cccgtcacgc gatcgatgac gtagaagaag
      901 ccgttacgat tcgcgaccat gacgacgcgc cgttcgccgc cctgccacgg cagcgtcgcc
      961 tgcacgggca cgtggttcga gtcccagtcg tgcgtgtcgt gcggcgtgaa ttgatagtgc
     1021 cagcgcagtt tgccggtggc cgcctcgatc gcgagcaacg agtccgtgta gagattgtct
     1081 ccctggcgat ccacgccgta gaaggtgggg ttcgggttgc ccgtgcccca atagagcagc
     1141 cccaagttcg ggtcgtagct gcccgtcatc cacgtgccgc cgccgccgcg cgccgcgctc
     1201 tgcacacccg gccaggtgtc gctgccgggc tcgccgggtg cgggcacggt atacaagcgc
     1261 cagatccggg cgccggtcgc gggatcgtag gcgtcgacga agccgcgggt tgggtattcg
     1321 ccgcccgaga tgccgacgat gaccttgtcg tcgacgacga gcggcgcaag cgtcgccgcg
     1381 tagccgatgc gatagtccgc gagctcgacg tcccagagca cgacaccgga gcgccgatcg
     1441 agcgcgacca agtgcgcatc ctgcgagacc aggaacagcc gctcgcccaa agtgccgaag
     1501 ccacggttca cgggcgcact ggcgccgtac gtgagattgt ccggcaaggt gcgccgatac
     1561 tcccagaacg gccggcccgt ccgcgcgtcg atggcccatg cgtaaccgtt ggcgcccgtc
     1621 gcgtacaaca caccgtcgat ggccagcggc gtcgtctcga agccgcgacc gcgcgcatag
     1681 gttcccgatt ggaacgtcca ctgggccttg agccgagcca cgttacgcgg cgtgatttgc
     1741 ttgagcgggc tatgccgcgc gccgctgtag tcaccggaaa acgtcaccca gcgcgttgga
     1801 ttcgcaagcc ctgcttcgat gtccgctgcc gtgaccccgg acggactctc gggcgtctgc
     1861 gcgagcgcgg tcgaagcggt cagtaatatg gccggaatcg acaaccaatt catcggcact
     1921 cgtctcattg attcgggcct catgggttcg cctgcgctcc ccgcagagtg ccgagatacg
     1981 cgagcaaatc ttcgaaagcg gcatcgttca agcgactcgc gtcgaagacc ggcatcaacg
     2041 actctgtgct gcgctcgagc gcgcggagct cgctcttccg aaacgcgcgc aactcgccgt
     2101 tcagggtgac gacctgcagc gagaacgcgt cctcgctctt gacgacgccc tccatgcgct
     2161 cgccggccct cgtctcgacg ctgacgccgc gaaagcccaa cgcgacggag gcactcggat
     2221 cgcgcacggc ggccgtcaac gcggcgcgcg accgcaacgc gccgatgctc gtgaggtccg
     2281 ggccgagcgc cccaccctcg ccgcgcacct gatggcagcc ggcgcagtcg gcagcgaaca
     2341 cggcgcggcc gcggctcgcg tcgcccgtgc tttgcagtgg cggcatgacg ctgatgctgc
     2401 gtagatgggc gacgatggcc cagatctcgt tatcgggtgc agcgttcggc ggcatgctgc
     2461 tcccgggcac accgttgcgg atgaccagga acgcgctctc gtcggtctcg ccgcgcgccc
     2521 accgtcgcgt gagatcggga ccaagctgcc ccttcgcgtc gggtccgtgg cagagggcac
     2581 agcgcgcggc gaacagcccg gcgccggcgc ggatcgccac ttcgtcgccc tcgaacggat
     2641 tgcgctgctc tgcgcttgcg ctgagcgccg ccgcgcagag cgaaagccac aacgtcgaga
     2701 caacgcgctt cgctacgcgt cgcgtcatgc gctcgcctgg tcgagctcgg ccatgtcggc
     2761 ctcggcgagc ttcaggtcga gcgcgcccat gagctccgtg acctgcgcga cggaggtcgc
     2821 gctcgcgatc ggcgccgcga ccgcgggctt cgccatgacc caggcgagcg cgacctgcgc
     2881 gcagctcgcg ccgtggcgcg ccgcgacggc gtcgagcgcc gcgagcaccg gcttcccctt
     2941 gccttcgaga ttcttgacgg ccccgccgcc acgcacgctc ttcgtcttgt cggcgctcgt
     3001 gcggtactta cccgtcagaa agccgctcgc gagcggatag tacggcgtga tcgcaacgcc
     3061 ttcgcgctgg caaagcggca tcaagtcctt ctcgatcgcg cggttcagca agctgtattc
     3121 gggttgttgc gcagcgaagc gcgccatacc cgagcgctcg ctttccgcga gcgccgcctc
     3181 gaagcgtgcg acgccgaagt tcgacgcgcc gacggcgcgc accttgccgg ccttgaccag
     3241 cgtgtcgagc gcgccgagat actcgtcggc cggcgtctgc tcgtcgtcgc ggtgcagcca
     3301 aaagagatcg atcgattcga gcccgagccg gcgcagcgag tcctcgcagc cttcgatgat
     3361 gttcgagcgg ccgagcccct tgcgcttctc ccaccaaccg accttcgtgc agacgagcac
     3421 gtcgtcgcgt cgcccacgct tcttgagcca gcgtccgatg accgtttcgc tttcgccgcc
     3481 ttggtgcccc ggaacccaat acgagtagcc gtccgcggtg tcgatcgcgt tgccgccgtg
     3541 cgcgacgaac gcgtcgagaa ttgcgaacga tgtcggctca tcggctgtcc agccgaacac
     3601 gttgccgccc aaaacgagcg gcgtgacgct gagcgacgat cccccgagcc ttctctgttg
     3661 catgccgtgg cgagcgtagc acgcggcccg tacaacgggg cggccggcgc cgttgacaga
     3721 ctcgcggtaa cgcaccggac attaccaagt gcgccagctc cgttgaccct cgttcacgcg
     3781 tgagccta
//
LOCUS       DATLEE010000281         4253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_322768, whole genome shotgun sequence.
ACCESSION   DATLEE010000281 DATLEE010000000
VERSION     DATLEE010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322768"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VM692_05915"
     CDS             complement(<1..560)
                     /locus_tag="VM692_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HUQ51737.1"
                     /translation="MLDNTSVCDSAAHLLELEKAFGVFTRVSNELQSSYQALEQRVAR
                     LTAELEDARRASQSAAAEAERTAQRLRSLLATLPGGVVVVDDSGEIQEHNRIAADILG
                     GDLVGESWQRVVKDVLRNPPSADGDWLTRDGRRIALAASSLPQESVKIILLTDVTDTR
                     ALQELAARNQRLSAIGKMAASLAHQIR"
     gene            complement(769..1752)
                     /locus_tag="VM692_05920"
     CDS             complement(769..1752)
                     /locus_tag="VM692_05920"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUQ51738.1"
                     /translation="MIVANEARQEFPVGRSPAIHGLDQLMRQVAGYDSTVLIRGESGC
                     GKELVARRIHELSARRRGPFVPVNCGAIPRELLESELFGHEKGAFTGALTTRIGRFEL
                     ADGGTLFLDEIGDMSFDMQVKLLRVLQERAFERVGSGEQRRANVRILAATHRDLEARA
                     LAGEFREDLFFRLNVFPIVVPPLRERVDDLPALIDDLVRRGEAEGRPSIAFTETALDC
                     LSAYSWPGNVRELQNLIERLAILYPNQTIDREQLPVPIGERRRNVQLSVATIPQTGLD
                     LREYLGNIEKQLIRSALDQADGTVAHAAKLLKLQRTTLVEKLRKYELVAHA"
     gene            complement(2024..2344)
                     /gene="fliT"
                     /locus_tag="VM692_05925"
     CDS             complement(2024..2344)
                     /gene="fliT"
                     /locus_tag="VM692_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HUQ51739.1"
                     /translation="MSPLVATVLDLTDQVQAAIDGGDWTRAQELETERRRLLEQLAAA
                     PDAGDLKPTFAALVARNHRLVGLVEHQKRRVLREAAVARSGHDAAAAYADVRGSAASL
                     AESA"
     gene            complement(2341..2730)
                     /gene="fliS"
                     /locus_tag="VM692_05930"
     CDS             complement(2341..2730)
                     /gene="fliS"
                     /locus_tag="VM692_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HUQ51740.1"
                     /translation="MAFTGSQQYRTMSTHGNLADASPHRVVQAMLDATLSRIVEASGH
                     LERGEVAAKGEKIGKALAILEALMLGLDKERGGELAHNLERLYDYASRTLLKANLENR
                     SDLLKEVTSLLREIKLGWDGIAPAKAG"
     gene            complement(2743..4125)
                     /gene="fliD"
                     /locus_tag="VM692_05935"
     CDS             complement(2743..4125)
                     /gene="fliD"
                     /locus_tag="VM692_05935"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HUQ51741.1"
                     /translation="MGSIVTAGVGSGLDVTSLVKQLVQAEGGPKTLRLNAEEAKVQAK
                     LSALGTLRSALATFRGTVTTLKNIDKFQGRQASLSTLDFVKVSAKSSAVPGSYEIEVQ
                     QLATTHKLQSSGATATAATVVGTGTLHFEIDEQQFDVVIDAESNTLAGIAAAINDSAV
                     GAKVLATVITGSGEARLTLSARASGADNEMTVTQNGGDAGLVALVAGLAPLQDALDAK
                     VLIDGVPVTSSTNTVSGAVAGVEIALIKANDVGVTTEVTVGYDRAAARKTIDDFVKSY
                     NAVVDAIKSVSGYNVETREGGPLFGDAGVRNIVFQLRRELTANVTGLSASFDMLGEIG
                     ITADLNGKLSVDSADLDAAFTADFDGIGELFATEDVGLAVKLDKLLAPYLDSAGVFDS
                     RTAGLKSSIDVLSERREELNTRLVALHARYTKQFNALDSLLSQLQGTSNFLNQQLSKL
                     PGFTSQSGKG"
BASE COUNT          686 a         1384 c         1379 g          804 t
ORIGIN      
        1 cgaatctggt gcgcgaggct cgcggccatc ttgccgatgg cgctcaagcg ctggttgcgg
       61 gcggccaact cttgcagcgc ccgcgtgtcg gtgacgtcgg tcagcaagat gatcttgacg
      121 ctttcctgcg gcaacgagct cgcggcgagc gcaatgcgtc tgccgtcgcg cgtcagccag
      181 tcaccgtccg ccgacggcgg gttgcgcagg acgtctttga cgacgcgctg ccacgactcg
      241 ccgacgaggt cgccgccaag aatgtccgcg gcgatgcggt tgtgctcctg gatctcgccg
      301 ctgtcgtcga cgacgacgac tccaccgggt agcgtcgcga gcagcgagcg cagccgctgc
      361 gccgtacgct ccgcctcggc ggccgcgctc tggctcgcgc gccgcgcgtc ctcgagctcg
      421 gcggtcaagc gcgcgacgcg ctgctcgagc gcttggtacg agctctgcag ctcgttcgac
      481 actcgagtga aaacgccgaa cgctttctcg agctcgagga gatgtgccgc ggaatcgcaa
      541 accgacgtgt tgtcgagcat tggggtacct cgggggcacc cagagcaaga accatgcccg
      601 agatcaagat cttgtcgaat cagcgagtta gcgattgcga gcgctcgtgc ccgtcaattc
      661 gttgacggtg gaggagggag gagtaggcgg cgaagcggcg gaaaatcgac gcatcgttga
      721 atgagcgcgc agcgctcttc gacacgtctt tacgtctttg aacgagtctt atgcgtgagc
      781 gacgagctcg tacttgcgca gcttctcgac gagcgtcgtg cgctgcagct tcaacagctt
      841 ggcggcatgc gccacggtgc catcggcttg atcgagcgcg ctgcggatca gctgcttctc
      901 gatgttgccg agatactcac gcagatcgag cccggtttgc ggaatcgtcg cgaccgaaag
      961 ctggacgttg cggcgccgct cgccgatcgg gacgggaagc tgctcgcggt cgatcgtctg
     1021 attcggatag aggatcgcga gccgttcgat gagattctgc agctcgcgca cgttgccggg
     1081 ccacgagtaa gcggagagac aatcgagcgc cgtctccgtg aacgcaatcg acggccggcc
     1141 ttcggcctcg ccgcggcgta cgagatcgtc gatgagtgcc ggcaagtcgt cgacacgctc
     1201 gcgcaacggc ggcacgacga tcggaaagac gttgagcctg aagaacaaat cctcgcggaa
     1261 ctcgccggca agcgcgcgcg cctcgaggtc gcgatgcgtc gcggccagga tgcgcacgtt
     1321 ggcgcggcgc tgctcgccgc tgccgacgcg ctcgaacgcg cgctcctgga gcacgcgcaa
     1381 gagcttgact tgcatgtcga agctcatgtc gccgatctcg tcgaggaaca aggtgccgcc
     1441 gtcggcgagc tcgaaccgcc cgatgcgcgt cgtcaaagcg cccgtgaagg cgcccttctc
     1501 gtggccgaag agctcgctct cgagcagctc gcgcgggatg gcgccgcaat tgacggggac
     1561 gaacgggccg cgacggcgcg ccgaaagctc gtgaatgcgc cgcgctacga gctctttgcc
     1621 gcagcccgac tcgccgcgaa tcagcacagt gctgtcgtaa cctgccacct ggcgcatgag
     1681 ctggtcgagc ccatggatgg cgggggagcg accgaccgga aactcctgac gagcctcgtt
     1741 cgcaacgatc attcacgtac cttcgcggcc tcttgccgcc ttgcttcgtc gccgccctag
     1801 gcttcgtggc catcgggctt gcgaccaatc gcgcgactcg cattcgctgc aacccactgt
     1861 tttttcagtg tttgatccgc gctcgcggtg cgcgggccag ctcgaaaccg gggtttggcc
     1921 cggtgcaatc tgactgctgg gttatgttat gcagcccgcg cgaaaaatat gtgcagtagc
     1981 tcgcaatttt gtatgtgcga gctaaatgtt ttggttgtaa cggctatgca gactctgcca
     2041 gcgacgcggc ggagccgcgc acgtcggcat aggctgccgc agcatcatgg ccggaccgcg
     2101 ctacggcggc ttcgcgcagg acgcgccgct tctgatgctc gacgagcccg accaagcggt
     2161 gattccgcgc cacgagcgcc gcgaacgtcg gcttcagatc gccggcatcg ggcgccgcgg
     2221 cgagttgctc gagcaaccgc cgccgttcgg tctcgagctc ttgcgcccgc gtccagtcgc
     2281 cgccgtcgat cgcggcttgc acctggtccg tcagatcgag gacggtggcg acgagtgggc
     2341 tcatccggcc ttcgccggcg cgatgccgtc ccagccgagc ttgatttcgc gcagcagcga
     2401 ggtgacttcc ttcagcagat cggagcggtt ctcgaggttc gctttcagca acgtccgcga
     2461 cgcgtagtcg tagagccgct cgaggttgtg cgcgagctcg ccgccacgct ccttgtcgag
     2521 ccccagcatc aacgcctcga ggatcgcgag cgccttgccg atcttctcgc ccttcgcggc
     2581 cacctcgccg cgctcgagat ggccgctcgc ttcgacgatg cggctcagcg tcgcgtcgag
     2641 catcgcttgc acgacgcggt gcggcgacgc atcggccagg ttgccatgcg tgctcatcgt
     2701 tcgatattgc tgcgatcctg tgaatgccat gtgcgttcct cgttagcctt tgccgctctg
     2761 cgacgtgaag cccggcagct tgctcagttg ctgattcaga aagttgctgg tcccttgcag
     2821 ctgcgagagc aggctgtcga gcgcgttgaa ctgcttggta tagcgagcgt gcagcgccac
     2881 gagccgggtg ttcaactcct cgcggcgctc gcttagcacg tcgatcgacg atttgaggcc
     2941 ggccgtccgg ctgtcgaaca cgccggcgct gtcgaggtac ggagcgagca gcttatcgag
     3001 cttcaccgcg aggccgacgt cttccgtggc aaacagctcg ccgatgccgt cgaaatccgc
     3061 cgtgaaggcc gcgtcgaggt cggcactgtc gacgctgagc ttgccgttca aatccgccgt
     3121 gatgccaatc tcgcccagca tgtcgaagga ggcgctgagc ccagtgacgt tcgccgtcaa
     3181 ctcgcggcgc agctgaaaga cgatgttgcg aactcccgcg tcgccgaaca acgggccgcc
     3241 ctctctcgtt tcgacgttgt agcccgacac gctcttgatc gcatcgacga cggcgttgta
     3301 gctcttcacg aagtcgtcga tcgtcttgcg cgcagccgca cggtcgtacc cgaccgtgac
     3361 ttcggtcgtg acgcctacgt cattcgcttt gatgagcgcg atctccacgc cggccaccgc
     3421 accgctcacg gtgttcgtgc tgctggtgac ggggacaccg tcgatgagca ccttcgcgtc
     3481 gagcgcgtct tgcaacggcg ccagcccggc aacgagagca acgagacctg cgtcgccgcc
     3541 gttctgcgtg accgtcatct cgttgtcggc gcccgatgcc cgcgctgaca gcgtgaggcg
     3601 cgcctcgccg gagcctgtga tgaccgtcgc gagcactttg gcgccgacgg ccgaatcgtt
     3661 gatggcggcc gcgatgccag ccagcgtgtt gctctcggcg tcgatgacga cgtcgaactg
     3721 ctgctcgtcg atctcgaagt gcaacgtacc cgtgccgacc acggttgccg ccgtcgcggt
     3781 ggcgccgctc gattgcagct tgtgggtcgt cgcgagttgc tggacctcga tctcgtagct
     3841 gccgggcacc gcactcgact tggcactgac cttgacgaag tcgagcgtcg ataaagaggc
     3901 ttgccggccc tgaaacttgt cgatgttctt cagcgtggtc acggtgccgc gaaatgtcgc
     3961 gagcgccgag cgcagcgtgc caagcgcgga cagtttcgcc tggaccttcg cctcttcggc
     4021 gttcagtcgc agcgtcttcg gcccaccttc ggcttgcacg agctgtttga cgaggctcgt
     4081 cacgtcgagg ccggagccta cgcctgcggt gactatgcta cccatgactc atccgttgtc
     4141 gccgaattac ggcgtccatt gatctcgctt ggcgcagagc aagatgtatg ccagctcagc
     4201 cgtgcgtatc gaccaacgag ccgaagcgct cgaggtgacg catcacagcg agc
//
LOCUS       DATLEE010000282         8396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1070384, whole genome shotgun sequence.
ACCESSION   DATLEE010000282 DATLEE010000000
VERSION     DATLEE010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070384"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VM692_05940"
     CDS             <1..1096
                     /locus_tag="VM692_05940"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUQ51742.1"
                     /translation="LEDGKFVLELAIFESGVEPEFHAWVTDGGEPVAPRDVTLTVELA
                     RLGGAVDRIDFAPQAEFLRGNQIVHEPHSFDVTVAARHEGREHRFAYSSYEGRTTIAA
                     DVARDAGIATATAGPGSISDELVLYGAIGPDTTRVRQVHGRFAGQIRSVARNVGDAVR
                     SGEALATIESNESLQTYTVTAPIAGIITQRHAAPGDQTDADSLFEIADFSSVWAELDV
                     FSRDRPRLREGMPVAISTDNGLAASGSIAYLAPVGNRASQSLTARVVLDNAGGLWTPG
                     QFVEGRVTITTTRVAIAVPLSALQRFRELDVVFAQVGETYEVRMLTLGRRDARFVEVL
                     DGLAPGTIYVTDNSYLIKADIEKAGASHDH"
     gene            1116..4247
                     /locus_tag="VM692_05945"
     CDS             1116..4247
                     /locus_tag="VM692_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HUQ51743.1"
                     /translation="MLEYLVDRSVRHRWLVLLAVAALAAFGAFNYTRLTIDAVPDITN
                     VQVQINTEAPGFSPLEAEQRITFAVETAMAGLPRLDYTRSISRYGLSQVTVVFEDGTD
                     IYFARQRVAERLQEARANLPPDLEPELGPIATGLGEIFSFILSVGPGAARPDGSPYDT
                     SDLHTVMDWVVRPQLARVPGVTEVNMVGGHERQYVVAPRPAALLAFRLTLADLATALE
                     RNNVNVGAGYIERFGSQYLVRVPGQVASLDDIRAIRVATHEGTPVVVGDVADVEIGKD
                     LCTGAATMNGEEVVLGTVFMLMGENSRIVSQAAADKLAEINRSLPEGLRLDAVYDRTT
                     LVDKTIATVRTNLLEGALLVVAVLFALLGNLRAALATTLVIPLAMLLTITGMVEARVS
                     GNLMSLGALDFGLIVDGAVIIVENCLRRLAAAQGGGAPLALAQRLEVVREATTEVVRP
                     SLFGLFIILIVYLPIFALSGVEGKMFHPMALTVSLALTAALVLSVTFVPAAVAVLVRG
                     KVAQHENIVVRGAKRVYAPLLRTALRFRVAVVVAAALLVAGAASLATRMGSEFIPSLD
                     EGDIALHALRIPSTGIDQAIGMQLQLDSRLARFPEVERVFAKLGTADIATDPMPPSVA
                     DTFVILKERTLWPNPRKPKDDLLREIEEAAARVPGNNYEFTQPIEMRFNELISGVRAD
                     VAVKVYGDDLPTLLELGEQIEGVLSGVAGGADVALEQMSGLPMLTVQPRRAVLARYGL
                     DVADVQDVVATAIGGKTVGQVFEGDQRFDLVIRLPETLRNDPLALEALPVPLPSGGYV
                     PLRELAVVEVVEGPNAINRENGKRRAVVTANVRGRDLGSFVADARARVEAQVELPAGY
                     WLEFGGSFEQLISGAQRLQLVVPITLLLIFTLLYLAFRSFSDSLLIFSGVPLALTGGV
                     LALWLRDIPLSISAGVGFISLSGVAVLNGIVLVSFIRTLLADGLSLEDAIVKGALTRL
                     RPVLMTALVASLGFVPMAFNTGVGAEVQRPLATVVIGGIVSSTLLTLLLLPALYWLVH
                     APRTVGTQWRRGL"
     gene            4341..6116
                     /locus_tag="VM692_05950"
     CDS             4341..6116
                     /locus_tag="VM692_05950"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUQ51744.1"
                     /translation="MNKNKNKDIEVSGRALDITELPTGEYPALSEGDGNTAELPALAQ
                     PETQPVAGEKAAADAEKASFWLAHLESEVTRLHAKWQTIDAEFKSREARAAELQQEIA
                     AREATIAQLNADLQRDAASLEAAGERLAGKDAELAALTEDRRARDERIAALATELADA
                     EVNRKAALAKIVLAEAETARLNDVLAKERGMAADLADQNAQLTVEQHRLNGKLQDLEA
                     YINGRHDSWSTLNAKLAEYKDALAGMEKTVQARDAEHSRLDDEKRGLSAKILELERQC
                     SELAGRRKEREEAYDELQQRLAAHFAQMEQLKTEHAGRIKELDHAAGAALGNQRQIEA
                     LQGDIKGRDARIATLEGEVQHAKGVIAELTTANGELTQRVGALDQGLVDRSLDLHALR
                     EELRKTREQLTALEGHAAELGRLRAEAVAVSAERDRELTAQKDLVANLTADLRAKKAA
                     TELLERSVGRITDLGASLAALDKEMQSGDDEPQRDGQQPSAGNGGRKLVTTIGGEAIQ
                     YPIFKNVMTIGRGHESDIRIPSHFVSRIHAKISTNGMATIIEDAGSKNGLVVNSERVQ
                     RHVLHHGDVVNIGGELNLKFVDAMH"
     gene            complement(6129..7961)
                     /locus_tag="VM692_05955"
     CDS             complement(6129..7961)
                     /locus_tag="VM692_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51745.1"
                     /translation="MRTTGTTLAALSVTAFAATAAAQTDVAGDWDHSGGGIFGFQEEF
                     LDRGGGPDLGDYAGLPINDALRYKASLYSPSWLSIPEHQCIPHPSTYQYRSPGGLSIV
                     KEYDPVTQRLVAYHTYGSYGLARTIFMDGRPHPPANARHTYEGFSTGKWEGNKLVVET
                     THLKAGFLRRNGIAHSDRARMTEYFLRHDNWMTVVTGVDDPVYLDEPFVRSTDYRLNG
                     QPNTRLNEFGGFVNGGDSEGFGASDVFFKCAPTEEIEIEPGSVPSFMPGANEHLDMFA
                     ARHNVPLEAALGGSATLYPEFARRIRELGPGVGVAPRSSGAPPRRPAAADAADTAVTS
                     LHVAGQVWLISAGGHNITVQIGAEGVLVVNPGSADVAPAVAEEIRRLAPDRRIHVIVA
                     TNDAPAHVGGNALLAAGPTPAAQRAAIIAHENASLRLASKDLPSGAAPTDTFFRGTRE
                     LYFNDEPIDIVHVPAATSDGDVLVFFRKSDVVVAGDVLEDLTYPVIRVQDGGTVDGTI
                     AALNRILGITVAAWRAQGGTMVVPNRGRVYDEGDVAEYRDMLTIIRDRVRDAVAKKQS
                     LADLRAAGIARDYDGRYGAPTGPASAAEFLASVYASATSSPRAE"
     gene            complement(7985..>8396)
                     /locus_tag="VM692_05960"
     CDS             complement(7985..>8396)
                     /locus_tag="VM692_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51746.1"
                     /translation="PPGAFAFPVAPNRGVAAARSGPAAGPPSGAPPATAARPPPQGGL
                     KVVTTKLKPGYLRKNGVPYSDQAVVTEYFDRLSMFGNDYLQVVTVVVDPVYLTAPFVV
                     SNQFKREPDGSKWQPTPCATDAPLGTLQAPPALF"
BASE COUNT         1377 a         2821 c         2879 g         1319 t
ORIGIN      
        1 gctcgaggac ggcaagttcg tgctcgagct cgcaatcttc gagagcggcg tcgagcccga
       61 gtttcacgca tgggtaaccg acggcggcga gcccgtggcg ccgcgcgacg tcacgctgac
      121 cgtcgagctc gcaaggctcg gcggcgcggt cgaccgcatc gactttgcgc cgcaagcgga
      181 gttcttgcgc ggcaatcaga tcgtgcacga gccgcattcg ttcgacgtaa ccgtcgccgc
      241 acgtcacgag gggcgcgagc atcgcttcgc gtactcgtcc tacgaaggcc gcaccacaat
      301 cgcggccgac gtggcgcgcg acgccggcat cgccacggcg acggcaggcc ccggcagcat
      361 cagcgacgag ctcgtgctct acggcgcgat cggccccgac acgacgcgcg tgcggcaggt
      421 ccacggacgc ttcgcggggc aaatccgcag cgtcgcgcgc aacgtcggcg acgccgtgcg
      481 cagcggcgag gcgctcgcga cgatcgaatc gaacgagagc ttgcagacct acacggtcac
      541 tgcaccgatc gccggcatca tcacgcagcg tcatgctgcg cccggcgacc agacggacgc
      601 cgacagcttg ttcgagatag cggatttttc gtcggtctgg gccgagctcg acgtgttctc
      661 gcgcgaccgc ccgcgtctac gcgaaggcat gccggtcgcg atcagcaccg acaacgggct
      721 cgcggcttcg ggctcgatcg cctatctcgc gcccgtcggc aatcgcgcct cgcaaagcct
      781 cacggcgcgc gtcgtgctcg acaacgcggg cggcctatgg acgccggggc agttcgtcga
      841 gggtcgcgtc acgatcacga cgacacgagt ggcgatcgcc gtgccgttgt cggcgctgca
      901 gcggttccgc gagctcgacg tcgtgttcgc gcaggtcggc gagacctacg aggtgcgcat
      961 gctgacgctc ggccgccgcg acgcgagatt cgtcgaggtg ctcgacgggc tcgcgccggg
     1021 cacgatttac gtgaccgaca acagctatct gatcaaagcc gacatcgaga aagccggcgc
     1081 aagccacgac cactagcggc acgaggagaa cggccatgct cgagtatctc gtggaccgct
     1141 cggtgcgcca ccgctggctg gtcctgctcg ccgtcgccgc gctcgcggcg ttcggggcct
     1201 tcaattacac gcggctcacg atcgacgccg tgcccgacat cacgaacgtc caggtgcaga
     1261 tcaacaccga ggcgcccggc ttctcgccgc tcgaggccga gcagcgcatc acgttcgccg
     1321 tcgagacggc gatggccgga ttgccgcgac tcgactacac gcgctcgatc tcgcgctacg
     1381 gcctgtcgca agtgacggtc gtgttcgagg acggcaccga catctatttc gcgcggcagc
     1441 gcgtcgcgga gaggttgcag gaggcgcgcg cgaatttgcc gccggacctc gagcccgagc
     1501 tcggaccgat tgccacgggc ctcggcgaga tcttcagctt catcttgagc gtcgggcccg
     1561 gcgccgcgcg ccccgacggc tcgccgtacg acacgagcga cctgcacacc gtcatggatt
     1621 gggtcgtccg cccgcagctc gcgcgcgtgc ccggcgtcac cgaggtcaac atggtcggcg
     1681 gccacgagcg gcaatacgtc gtcgcgccgc ggccggcggc gttgctcgcg ttccgcctga
     1741 cgctcgccga tctcgccacc gcgctcgagc gcaacaacgt caacgtcggc gcaggctaca
     1801 tcgagcgctt cggcagccaa tatctcgtgc gcgtgcccgg ccaggtggcg tcgctcgacg
     1861 acatccgtgc gatccgcgtc gcgacgcacg aaggcactcc cgtcgtcgtc ggcgacgtcg
     1921 ccgacgtcga gatcggcaag gacttgtgca caggcgccgc gacgatgaac ggcgaagaag
     1981 tcgtgctcgg cacggtattc atgctgatgg gcgagaacag ccgcatcgtt tcgcaagcgg
     2041 cggccgacaa actggccgag atcaatcgct cgttgcccga gggcctgcgg ctcgacgccg
     2101 tttacgatcg cacgacgctc gtcgacaaga ccatcgcgac ggtccgcacg aatctgctcg
     2161 aaggcgcgct gctcgtcgtc gccgtgctgt tcgcgctgct cggcaacttg cgcgccgctc
     2221 tcgcgacgac gctcgtgatc ccgctcgcga tgctgctgac gatcacgggc atggtcgaag
     2281 cgcgtgtcag cggcaacctc atgagcttgg gtgccctcga tttcggcctg atcgtcgacg
     2341 gcgccgtcat catcgtcgag aactgcttgc ggcggctcgc ggccgcgcaa ggcggcggag
     2401 cgccgttggc gctcgcgcag cgcctcgagg tcgtgcgcga ggcgacgacc gaggtcgtgc
     2461 gccctagcct gttcggcttg ttcatcatct tgatcgtcta tttgccgatc ttcgcgctgt
     2521 cgggcgtcga gggaaaaatg ttccatccga tggcgctgac ggtttcgctg gcgttgacgg
     2581 cggcgctcgt gctcagcgtc acgttcgtgc cggcggccgt cgcggtcctc gtgcgcggca
     2641 aggtcgccca gcacgagaac atcgtcgtgc gcggcgcgaa gcgcgtctac gcgccgttgc
     2701 tgcgcactgc tttgcgtttc cgcgtcgccg tcgtcgtggc cgccgcgctg ctcgtcgcag
     2761 gagccgccag cctcgcgacg cgcatgggca gcgagttcat cccgagcttg gacgaaggcg
     2821 acatcgcgct gcacgcgctg cgcatcccga gcacgggcat cgaccaagcg atcggcatgc
     2881 agctgcagct cgactcgcgg ctcgcgcggt ttccggaggt cgagcgcgtg ttcgcgaagc
     2941 tcggcacggc cgacatcgca acggatccga tgccgccgtc ggttgccgac acctttgtga
     3001 ttctcaagga acgcacgctt tggccgaacc cgcgcaagcc gaaggacgac ttgttgcgcg
     3061 agatcgagga agcggcggca cgcgttcccg gcaacaacta cgagttcacg cagccgatcg
     3121 agatgcgctt caacgagctg atttcgggcg tgcgtgccga cgtcgccgtc aaagtctacg
     3181 gcgacgatct gccgacgctg ctcgagctcg gcgagcagat cgaaggagtg ctcagcggcg
     3241 tcgccggcgg cgccgacgtg gcgctcgagc agatgagcgg tttgccgatg ctgaccgtgc
     3301 agccgcggcg tgccgtgttg gcgcgctacg gcctcgacgt cgccgacgtg caggacgtcg
     3361 tggcgacggc gatcggcggc aagaccgtcg gtcaggtgtt cgagggcgac cagcgtttcg
     3421 atctcgtgat ccgcctgccc gaaacgctgc gcaacgatcc gctcgcgctc gaggccttgc
     3481 ctgtgccgtt gccgagcggc ggctacgtgc cgctgcgcga gctcgcggtc gtcgaggtcg
     3541 tcgagggccc gaacgcgatc aatcgcgaga acggcaagcg ccgcgccgtc gtgacggcca
     3601 acgtgcgcgg ccgcgatctc ggctcgttcg tggccgacgc gcgcgcccgc gtcgaagcgc
     3661 aagtcgagct gccggccggc tattggctcg agttcggcgg cagcttcgag caactgatct
     3721 cgggggcgca gcggctgcag ctcgtcgtgc cgatcacgtt gctgcttatc ttcactctgc
     3781 tctacctcgc gtttcgctcg ttcagcgact cgctgctgat cttcagcggc gtgccgctcg
     3841 cgctgacggg cggcgtgctc gcgctgtggc tgcgcgacat ccctttgtcg atctcggccg
     3901 gagtcggttt catctctctg tccggcgtcg ccgtcttgaa cggcatcgtg ctcgtgtcgt
     3961 tcatccgcac gctgctggcc gacgggctgt cgctcgagga tgcgatcgtc aaaggcgcgt
     4021 tgacgcgcct gcggcccgtg ctgatgacgg cgctcgtggc ctcgctcggg ttcgtgccga
     4081 tggcgttcaa caccggcgtc ggcgcggagg tgcagcggcc gctggccacg gtggtcatcg
     4141 gcggcatcgt ctcgtcgacg ttgctgacgc tgctgttgct gccggcgctc tattggctcg
     4201 tgcacgcgcc tcgtaccgtt ggaacccaat ggcggcgcgg cctgtagcgc cgcgggctgg
     4261 cagcggccac cttttgtcga aacgctaacg atatttgggc ccgtttcgtc tacgatcagg
     4321 cgactaaaag gttcggtgcc atgaataaga acaagaataa ggacatcgaa gtcagcggcc
     4381 gggctttgga catcacggag ctgccgacgg gcgagtaccc ggcgctcagt gaaggcgacg
     4441 gcaacacggc cgagctgcca gccttggctc agccggagac gcaaccggtc gccggcgaaa
     4501 aagctgccgc ggacgccgag aaggcgtcgt tctggctcgc gcatctcgag tccgaggtca
     4561 ctcggctcca cgccaaatgg cagaccatcg acgcggagtt caagagccgc gaggcgcggg
     4621 cagcggagct gcagcaggag atcgcggcgc gggaagcgac gatcgcgcag ctcaacgccg
     4681 atctgcagcg cgacgccgct tcgctcgagg ccgccggcga acgacttgcc ggcaaagacg
     4741 cggagctcgc ggcgctgacc gaggatcggc gcgcgcgcga cgagcgcatc gccgcgttgg
     4801 cgaccgagct cgcggacgcc gaggtcaatc gcaaagcagc gctcgcaaag atcgtgctgg
     4861 ccgaagccga gacggcacgc ttgaacgacg tgctggcgaa ggagcgcggc atggccgcag
     4921 acctggccga tcaaaatgcg cagctcacgg tcgagcagca tcgcttgaac ggcaagctgc
     4981 aggacctcga ggcgtacatc aacggcaggc acgacagctg gtcaaccctc aacgcgaagc
     5041 tcgccgagta caaggacgca ttggccggca tggagaaaac cgtccaggcg cgcgatgccg
     5101 aacattctcg actcgacgac gagaagcgcg gcttgtccgc gaagatcctc gagctcgagc
     5161 ggcagtgctc ggagttggcg ggccggcgca aggagcgcga ggaagcctac gacgagctgc
     5221 agcagcgcct tgccgcgcac ttcgcgcaaa tggagcagct gaagacggag cacgccggcc
     5281 ggatcaaaga gctcgaccac gcggcgggcg ccgcgttagg caatcagcgt cagatcgaag
     5341 cgctacaagg cgacatcaag ggccgcgacg cgcgcatcgc gacgctcgag ggcgaggtgc
     5401 agcacgcgaa gggcgtcatc gcggagctga cgactgccaa cggcgagctg acgcagcgcg
     5461 tcggcgcgct cgaccaaggc ctggtcgatc ggtcgctcga cctgcacgcg ctgcgcgagg
     5521 aattgcgcaa gacgcgtgag cagctgaccg cgctcgaggg gcacgccgca gagctcggca
     5581 ggctacgtgc ggaggcggtg gcggtgtcgg cggagcgcga tcgcgagctc acggcgcaga
     5641 aagacctcgt cgcgaacctg acggcggacc tgcgagcgaa gaaagccgcc accgaattgc
     5701 tcgagcgcag cgtcggtcga atcacggacc tcggggctag cctggccgcg ctcgacaagg
     5761 aaatgcagag cggcgatgac gagccgcagc gcgacggcca acagccgtcg gccggcaacg
     5821 gcggacgcaa gctcgtcacg actatcggcg gcgaagcgat tcagtaccca atcttcaaga
     5881 atgtcatgac gatcggccgc gggcacgaaa gcgacatccg gatcccgagc cacttcgtca
     5941 gccgcatcca cgcgaaaatc agcacgaacg gcatggccac gatcatcgag gacgccggca
     6001 gcaagaacgg cctcgtcgtg aactccgagc gcgtgcagcg gcacgtgctg caccacggcg
     6061 acgtcgtgaa catcggcggc gagctgaatc tcaagttcgt cgacgccatg cactagagct
     6121 ttcgtgcgct actcggcgcg cggcgagctc gtggcgcttg catagacgct cgcgaggaac
     6181 tccgcggccg aagccggtcc cgtcggcgcg ccgtagcggc cgtcgtagtc acgcgcgata
     6241 ccggcggctc gcaggtcggc gagcgactgc ttcttggcca ccgcgtcgcg cacgcgatcg
     6301 cggatgatcg tcagcatgtc gcgatactcg gccacgtcgc cctcgtcgta gacgcgcccg
     6361 cggttcggca cgaccatcgt cccgccctgc gcgcgccagg cggctaccgt gatgccgagg
     6421 atacgattta gcgccgcgat cgtgccgtcg acggtgccgc cgtcttgcac gcggatcacg
     6481 gggtacgtca gatcctcgag cacgtcgccc gcgacgacga catcggactt gcggaagaac
     6541 acgagcacgt cgccgtcgct cgtagccgcc ggcacgtgaa cgatgtcgat cggctcgtcg
     6601 ttgaaataga gctcgcgcgt gccgcggaag aacgtgtcgg tcggcgctgc accgctcggc
     6661 agatccttgc tcgccaagcg cagcgacgca ttctcgtgcg cgatgattgc ggcgcgttgc
     6721 gcggccggag tcggcccggc cgcgagcagc gcgttgccgc cgacatgagc cggcgcgtcg
     6781 ttcgtcgcga cgatcacgtg aatgcgccgg tcgggcgcga gccggcgtat ctcctcggcg
     6841 accgcgggcg cgacgtccgc ggagcccgga ttgacgacga gcacgccttc ggcaccgatc
     6901 tgcacggtaa tgttgtgccc gccggccgag atcagccaca cctgtcccgc gacgtgcagc
     6961 gacgtcacgg cggtatctgc ggcatccgcc gctgcgggcc ggcggggcgg cgcgccgctc
     7021 gaccggggcg cgacgccgac gcccgggccg agctcgcgga tgcgccgcgc gaactccggg
     7081 tacagcgtcg cgctgccgcc gagcgcggcc tcgagcggca cgttgtgccg tgccgcgaac
     7141 atgtcgagat gctcgttcgc gcccggcatg aacgacggca cggagcccgg ctcgatctcg
     7201 atctcctccg tcggcgcgca tttgaagaac acgtcgctgg cgccgaaccc ttcgctgtcg
     7261 ccgccgttca cgaagccgcc gaactcgttg agccgcgtgt tcggttgccc gttcagccga
     7321 tagtccgtcg agcgcacgaa cggctcgtcg agatagacgg gatcgtcgac gcccgtcacg
     7381 accgtcatcc agttgtcatg ccgcaagaaa tattccgtca tgcgcgcgcg atcgctgtgc
     7441 gcgatgccgt tgcggcgcag aaagcccgcc ttcaggtgcg tcgtctcgac gacgagcttg
     7501 ttgccctccc acttgcccgt cgaaaagcct tcataggtgt ggcgcgcgtt cgcgggcgga
     7561 tgcggccggc cgtccatgaa gatcgtgcgc gcgaggccgt agctgccata cgtgtgatac
     7621 gcgacgaggc gctgcgtcac gggatcgtat tccttgacga tcgacaagcc gccggggctg
     7681 cggtactgat acgtcgatgg atgcggaatg cactgatgct cgggtatcga tagccacgac
     7741 ggcgaataga gcgatgcttt gtaacgcagc gcgtcgttga tcggtaagcc agcgtaatcg
     7801 ccgagatccg ggccgccgcc gcggtcgagg aactcctctt gaaagccgaa gatgccgccg
     7861 cccgagtgat cccaatcgcc ggcgacgtcg gtttgggcgg ccgccgtggc cgcgaacgct
     7921 gtgaccgaga gcgccgccag agtggttccg gtcgtcctca tcgactgtct ccttggcgcg
     7981 cgcatcagaa taaggccggc ggcgcttgca acgtgccgag cggcgcgtcg gtcgcgcacg
     8041 gcgtcggctg ccatttcgag ccgtcgggct cgcgcttgaa ctgattactg acgacgaacg
     8101 gcgccgtcag ataaacgggg tcgacaacca ccgtcacgac ttgcagatag tcgttgccga
     8161 acatcgacag ccggtcgaag tactcggtca cgacggcttg gtcgctgtac ggcacgccgt
     8221 tcttgcgcag atagccgggc ttgagcttcg tcgtcacgac cttcaacccg ccttgcggcg
     8281 gcggccgcgc cgccgtagcc ggcggcgcgc cagagggtgg ccctgcggcc gggccgctgc
     8341 gcgcggccgc gacgccgcga ttcggcgcta ccgggaacgc gaacgcgccg ggcggc
//
LOCUS       DATLEE010000283         2443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_324487, whole genome shotgun sequence.
ACCESSION   DATLEE010000283 DATLEE010000000
VERSION     DATLEE010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324487"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VM692_05965"
     CDS             <1..1112
                     /locus_tag="VM692_05965"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HUQ51747.1"
                     /translation="SLSNCVNSGIRQIFILTQYKAHSLIQHVQRGWGYLHGELGEFID
                     IVPAQQQLGEIWYRGTADAVFQNLDIIHQHRPETVLVLAGDHVYKMDYGPLVAFHKES
                     GADITIGGVQVPISQAHEFGVMSLGADHRVVAFDEKPKDPQPMPGREGIALASMGIYV
                     INPEFLREALERDAALPDSQHDFGRNIVPEAVASRRVFAYPFEDVETKAQRYWRDVGT
                     VDAYYAANMELIHVSPELNLYDQAWPIWTYQQQVPGAKFILDEEGRRGLAINSMIAGG
                     CIISGAVVRESLLFFSVFVDEGSEVRRSVVLPEVEIGRSCYIANAIIDEGCSIPHGTV
                     IGRDHNADRENFHVTDQGIVLVTRGMLAKLRRSQA"
     gene            1167..>2443
                     /gene="malQ"
                     /locus_tag="VM692_05970"
     CDS             1167..>2443
                     /gene="malQ"
                     /locus_tag="VM692_05970"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HUQ51748.1"
                     /translation="MNSALAERRAGVLLHPTSLPGPGGRGTLGADARRFVDWLVAGGF
                     SVWQTLPLGVTDAYGSPYCLRSAYAGDPRFIDAGDLADFEVLPPGIAGAADRGARYAA
                     FAASARPAQRRAFAEFVQAQRRWLLPYGLFEVLSARFGQAPWWQWPDACRGADAAALR
                     ACLAADREQFRSLLFEQYLFELQWTALKQYANERGVLLFGDLPFYVDRNSVEVWWEPG
                     LFALDASGEPKAVAGVPPDYFNADGQLWGNPLYDWEAMQRNGFRWWVARMRAQLTRFD
                     LVRIDHFRALESYWEVPAGALSARTGQWRPGAGDALLTTLHGELGAMQLVAEDLGIIT
                     DEVRALRDRFALPGMVVLQFAFDGSPDNPHLPMHHKRNAVVYTGTHDNDTTVGWYAAL
                     DANARDVVHGKLELGSAPHVPEFLIEQAYASTAA"
BASE COUNT          385 a          823 c          814 g          421 t
ORIGIN      
        1 tctcgctgtc gaactgcgtc aactccggca tccgccaaat tttcatcttg actcagtata
       61 aggcgcactc cctgatccag catgtgcagc gcggctgggg gtacttgcac ggcgagctcg
      121 gcgagttcat cgacatcgtg ccggcgcagc agcaactcgg cgagatctgg tatcgcggca
      181 cggccgacgc ggtttttcag aacctcgaca tcattcatca acaccggccc gagaccgtgt
      241 tggttctcgc cggcgaccac gtctacaaga tggactacgg cccgctcgtc gcgttccaca
      301 aagagagcgg cgccgacatc acgatcggcg gcgtgcaggt gccgatctcg caagcgcacg
      361 agtttggcgt catgtcgctc ggcgccgatc atcgcgtcgt ggcattcgac gagaagccga
      421 aggacccgca gccgatgccg ggccgcgagg gcattgcgct tgcgtcgatg ggcatctacg
      481 tcatcaatcc cgagttcctg cgcgaggcgc tcgagcgcga cgccgcgctg ccggattcac
      541 agcacgattt cggccgcaac atcgtgcccg aggccgtcgc gagtcggcgc gtgttcgctt
      601 atccattcga ggacgtcgag acgaaagcgc agcgctattg gcgcgacgtc ggcaccgtcg
      661 acgcgtacta cgcggccaac atggagctca tccacgtgag ccccgagctg aacctctacg
      721 atcaggcgtg gccgatctgg acttatcagc aacaggtgcc cggtgcgaag ttcattctcg
      781 acgaggaagg gaggcgcggc ctcgcgatta actcgatgat cgccggcggc tgcatcatct
      841 ccggcgccgt cgtccgcgag tcgctactat ttttcagcgt gttcgtcgac gagggcagcg
      901 aggtgcgccg ctccgtcgtg ctgcccgagg tcgagatcgg ccgcagctgc tacatcgcga
      961 acgcgatcat cgacgaaggg tgcagcattc cgcacggcac cgtgatcggc cgcgaccaca
     1021 atgccgaccg cgaaaatttt cacgtcaccg atcagggcat cgtgctcgtg acccgcggca
     1081 tgttggcgaa gctgcgccgt agccaagcct gacgcggaca tgacgccggc gatagagcaa
     1141 cgcagggagt tgccgggcgg ccgcgcatga attcggccct cgccgagcgt cgggccggag
     1201 ttctactcca tccaacttcg ctgccgggac ccggcgggcg cggcacgcta ggcgccgatg
     1261 cacggcggtt cgtcgactgg ctcgtggccg gcgggttcag cgtctggcag accttgccgc
     1321 tcggcgtaac cgacgcgtac ggctcgccgt actgcttgcg ctccgcgtac gccggggatc
     1381 cgcggttcat cgacgcgggc gacctcgccg acttcgaggt tctgccccca ggcatcgccg
     1441 gcgcagcgga tcgcggcgcg cgctacgcag cgttcgcggc gagcgcgagg ccggcgcagc
     1501 gccgagcatt cgccgagttc gtccaagcgc agcgccgctg gctgctgccg tacggcctgt
     1561 tcgaggtctt gagcgcgcgg ttcggtcaag cgccttggtg gcagtggccc gacgcctgcc
     1621 gcggcgccga cgccgcggcc ttgcgcgcgt gtctcgccgc cgaccgcgag cagttccgct
     1681 cgttgctgtt cgagcaatac ctgttcgagc tgcaatggac cgcgttgaaa cagtacgcca
     1741 acgagcgcgg cgtcctgctg ttcggcgact tgccgttcta cgtcgatcgc aacagcgtcg
     1801 aggtctggtg ggagcccggg ctgttcgcgc tcgacgcgag cggcgagccc aaggccgtgg
     1861 ccggcgtgcc gcccgattac ttcaacgccg acggccagct ctggggcaac ccgctctacg
     1921 attgggaggc gatgcagcgc aacggctttc gctggtgggt ggcccgcatg cgcgcgcagc
     1981 tgacgcggtt cgatctcgtg cgcatcgatc actttcgtgc gctcgagtcg tattgggagg
     2041 tgccggccgg agcgctctcc gcgcgcacgg gccagtggcg gcccggcgcc ggcgacgcac
     2101 tattgacgac gctgcacggc gagctcggcg cgatgcagct cgtcgccgag gatctcggga
     2161 tcatcacgga cgaggtgcgc gccttgcgcg atcgcttcgc gctgccgggc atggtcgttc
     2221 tgcagttcgc gttcgacggc tcgcccgaca atccgcatct gccgatgcat cacaagcgca
     2281 atgccgtcgt ctacacgggc acgcacgaca acgacaccac ggtgggttgg tacgccgcgc
     2341 tcgacgccaa cgcgcgcgac gtcgtgcacg gcaagctcga gctcggctcc gcgccgcacg
     2401 tgccggagtt tttgatcgag caggcctacg cctcgaccgc ggc
//
LOCUS       DATLEE010000284         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1816556, whole genome shotgun sequence.
ACCESSION   DATLEE010000284 DATLEE010000000
VERSION     DATLEE010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816556"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..949)
                     /gene="argB"
                     /locus_tag="VM692_05975"
     CDS             complement(17..949)
                     /gene="argB"
                     /locus_tag="VM692_05975"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HUQ51749.1"
                     /translation="MTTRLNNPNERAIVITALRHALPYLRIFKNKVFVLKAGGDAFVA
                     PESTRALMEQIGILHQVGIRVVVVHGGGPQSTALAKRLGLTTQMVEGRRVTDAQTLEV
                     STMVLNGEINTRIVAVCRALGIPAIGLSGVDGGLIKATKRPPVQVAGQTVDYGFVGDI
                     LGLDTSILVKQLDNDLVPIVSPLSADDQGTVLNINADTVAAMIACDLKAEKFVMATGT
                     PGILESLEDPRSLISYIDRSGLRRLREEGKISGGMLPKVAAIERALSGGVPRVHVISY
                     AQADSLLLEVFTNEGTGTLVVNDTKALRPEEQAS"
     gene            complement(956..1954)
                     /locus_tag="VM692_05980"
     CDS             complement(956..1954)
                     /locus_tag="VM692_05980"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HUQ51750.1"
                     /translation="MRRFNDLADFSRDEIKDLIALASRLDAKPEPRALEGKVLSLLFL
                     SPSLRTLSSFQAAMIRLGGGSFVISPDMSIHGLESRYGIVMDGTAAEHVREAIPVIAS
                     YGDAIGVRAFAERRDLAHDLADTDFKNLTSLIDKPWINMESAIDHPCQSLADWKTMDD
                     LKVPENAKFVLSWAYHPRALPLAVPAATLHMAAKRGMNVTVLRPPGFELPPAIMDKAR
                     RAAAESGGSVSETADRREALTGAHIIYAKEWSSTRHYGDKLGDQALREELVHWCVDEP
                     WFADARPDAKLMHCLPVRRGVAVTDRILDGPRAVVIQEARNRLYAQMAVLYRMMKG"
     gene            complement(1941..>2180)
                     /locus_tag="VM692_05985"
     CDS             complement(1941..>2180)
                     /locus_tag="VM692_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51751.1"
                     /translation="IRASCCVGPVESIQGRGFLLGLKTRPKAAAVRDALLARDILVGT
                     SADPHMLRLLPALILATEHVQRLAAALEELIDAPL"
BASE COUNT          356 a          710 c          737 g          377 t
ORIGIN      
        1 gaggcgcgcc atgctgctag ctcgcctgct cctcgggtcg cagcgccttc gtgtcattga
       61 cgacgagcgt gcccgtgcct tcgttcgtga acacctcgag cagcaggctg tcggcctgcg
      121 cgtacgagat gacgtggacg cgcggcacgc cgcccgacag cgcgcgctcg atggcggcga
      181 ccttcggcag catgccgccg gagatcttgc cttcctcacg cagccgccgc aggccgctgc
      241 gatcgatgta cgagatcaac gagcgcggat cctcgagcga ctcgagaatc ccgggcgtgc
      301 ccgtggccat gacgaacttc tcggctttga ggtcgcaggc gatcatcgcg gcgaccgtgt
      361 cggcgttgat gttgagcacg gtgccttggt cgtcggccga gagcgggctc acgatcggca
      421 cgaggtcgtt gtcgagctgc ttgacgagaa tgctcgtatc gagccccaag atgtcgccga
      481 cgaagccgta gtcgacggtc tgccccgcca cttgcacggg cgggcgcttt gtcgctttga
      541 tgagcccgcc gtcgacgccg gacaggccga tcgccggaat gccgagcgcg cggcacacgg
      601 ccacgatgcg cgtgttgatc tcgccgttca ggaccattgt cgacacttcg agcgtctgtg
      661 cgtcggtcac acgccggccc tcgaccatct gcgtcgtgag cccgaggcgt ttcgcgagcg
      721 ccgtcgactg cgggccgccg ccgtgaacga cgacgacgcg gatgcccacc tggtgcagga
      781 tgccgatctg ctccatcagc gcgcgcgtgc tttcgggcgc aacgaacgcg tcgccgccgg
      841 ctttgaggac gaacaccttg tttttgaaga tgcgcaagta cggcagcgca tgacgcagcg
      901 ccgtgatgac gatcgcgcgt tcgttcgggt tgttgagtcg ggtagtcatc tagttctagc
      961 ccttcatcat gcgatagagc acggccatct gcgcgtacag acggttgcgc gcctcctgaa
     1021 tcacgacggc gcgcgggccg tcgagaattc ggtccgtcac cgcgacgccg cggcgcacag
     1081 gcaggcagtg catgagcttg gcgtcgggcc gcgcgtcggc gaaccacggc tcgtcgacgc
     1141 accagtgcac gagctcttcg cgcaaggctt ggtcgccgag cttgtcgccg tagtggcgcg
     1201 tcgacgacca ctcctttgca tagatgatgt gcgcgcccgt cagcgcttca cgtcggtccg
     1261 cggtctcgct gacgctgccg ccggactcgg ccgccgcgcg ccgcgccttg tccatgatcg
     1321 cgggcggcag ctcgaagccg ggcgggcgca gcacggtgac gttcatgccg cgtttcgcgg
     1381 ccatgtgcag cgtcgcggcc ggcaccgcga gcggcagcgc gcgcgggtga tacgcccacg
     1441 acaggacgaa cttggcattc tcggggacct tcaagtcgtc catggttttc cagtccgcga
     1501 ggctctggca tggatgatcg atcgccgatt ccatgttgat ccacggctta tcgatcaagc
     1561 tcgtgagatt cttgaaatcg gtgtccgcga gatcgtgcgc gagatcgcgc cgctcggcga
     1621 acgcgcggac gccgatcgcg tcgccgtacg aagcgatgac ggggatcgct tcgcgcacgt
     1681 gctcggccgc cgtgccgtcc atgacgatgc cgtagcgcga ctcgaggccg tgaatggaca
     1741 tatcgggcga gatcacgaac gagccgccgc cgagccggat catcgcggcc tggaacgacg
     1801 acagcgtccg caacgacggg ctcaagaaca agagcgacag caccttgcct tcgagcgcgc
     1861 gcggctccgg cttcgcgtcg aggcgcgagg cgagcgcgat gagatctttg atttcgtcgc
     1921 gcgagaagtc ggccaagtcg ttaaagcggc gcatcgatca gttcctccag ggcggcagcc
     1981 aggcgttgta cgtgttcggt cgcgagaatc agcgccggca ataggcgcag catatgcgga
     2041 tcggcgctcg tgccgaccaa gatgtcgcgc gcgagcaaag cgtcgcgcac ggcggcggcc
     2101 ttgggacgcg tcttcaagcc gagcaaaaag ccgcggcctt gaatgctttc gaccggccca
     2161 acgcagcacg acgcacggat
//
LOCUS       DATLEE010000285         4142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2563845, whole genome shotgun sequence.
ACCESSION   DATLEE010000285 DATLEE010000000
VERSION     DATLEE010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2563845"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..2145
                     /locus_tag="VM692_05990"
     CDS             220..2145
                     /locus_tag="VM692_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ51752.1"
                     /translation="MRLPAQRELGLAIFAALCMATADAADADDAAPGRSLADLSLEEL
                     GDIEVLSVSREPERLAAAPSSIFVITNEQIRRAGPTSLGEALRIAPNLQVARINSVGF
                     AITARGFNNSLANKLLVLVDGRTVYTPLFSGVFWDQQDLVLADVDRIEVISGPGATLW
                     GANAVNGVINVITRSAAETQGGFVSAGGGNFESQATLRYGGRLGGSGHYRAYAKTASF
                     DATQRASGASALDAWTRQQVGFRADWDLGRDSVTLQADVADGRSQDRGTAAGFAFGRI
                     ETSGANVLGRFKRDLGDGSSLSVQSYLDHSERDDLLFFRPRRDTFDVEAQHGFERGAN
                     DVIWGGGYRHMSDEIGTGFITTFIPGSRDLEWANVFVQDRIQLRANLETTVGLKLERN
                     DYTGTESLPSARLAWRPSDDRLLWTAVSRAVRAPSRVDREVFFPGTPPFIVVGGPNFQ
                     SEVANVVELGYRAQPAPGLSYSATLFRHDWDKLRSGTAVPVQLENRIEGDVYGVEAWA
                     NWRVTERWELSGGLNTLDKDLVLEPGSTDPAGVNNPTLANDADFQWTLRSALQLPIGL
                     QLDVAVRRVDSLPNPRVPRYTAVDARLAWDHGANLTVDLNVRNLFDAKHPEFEAAPNR
                     SELPRTVLLKVDWRFGG"
     gene            2283..2972
                     /locus_tag="VM692_05995"
     CDS             2283..2972
                     /locus_tag="VM692_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUQ51753.1"
                     /translation="MTASSTASSLPQRDAKPAIVALALRDKTLAATLAQRLAAGLGAD
                     VLQFRSSSPAQLVPWLDDARADVLLLDQRWLHRLAGAATRRLGARWPAQRVLLVGDRA
                     CRALAEHVVRQRLHGFLLANEAAELCAKAVGVVERGEIWVPRTLLVELLFEHFDSAGE
                     GEAPSDVKLTRRETEVVGYVRRGFANKQIAESLAIRVDTVKKHLRSAYTKLGVHRRSE
                     IMMGAVRLAGT"
     gene            3051..3461
                     /locus_tag="VM692_06000"
     CDS             3051..3461
                     /locus_tag="VM692_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51754.1"
                     /translation="MGYAVGIALALFVSLFARLTGLDRDRAFYPTIAIVIALLYVLFA
                     VMGASSHALLVDSIPMAAFVLLAVLGFKLNLWLVVACLAGHGIFDLFHPHVIDNPGVP
                     EWWPSFCMTYDLVAAAFLAGLLLRAKIAVRVASR"
     gene            complement(3566..3772)
                     /locus_tag="VM692_06005"
     CDS             complement(3566..3772)
                     /locus_tag="VM692_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51755.1"
                     /translation="MTRAYVALGPDNQEKIIDFLQTLVLFPPDDTASNLNPGVPSSDD
                     PQLPGNHGSISLSPLFQIPSEGVE"
BASE COUNT          628 a         1406 c         1398 g          710 t
ORIGIN      
        1 ggcgcagggc gtgctgctgc tttgccgcgg gcaggaggtc acgcccgccg tcaaggcgca
       61 tctcgagcgc ttctacgctg tgggcgcgtt ggcgggggcg atcctggtct cgggccgccc
      121 ggcgcggccg cggcttgccg ggccgtgacg tagttagcag ccgcacgcaa atatccggcg
      181 gtagatttgg cgctctaacg tgtatccgaa ggagcgaacg tgcggctacc cgcacaacga
      241 gagctaggcc tcgcgatctt cgcggcactc tgcatggcga ctgcggatgc tgcggacgcg
      301 gacgatgccg cgcccggtcg cagcctcgcg gacctctcgc tcgaagagct cggcgacatc
      361 gaggtgcttt cggtttcgcg cgagcccgag cggctcgcgg cagcgccgtc gtcgatcttc
      421 gtcattacga acgagcagat tcgccgcgcc ggccccacga gcctcggcga agcgctgcgc
      481 attgccccga atctgcaggt ggcgcgtatc aattccgtcg gcttcgccat cacggcgcgc
      541 ggcttcaaca attcgctcgc caacaagctg ctcgtgctcg tcgacgggcg caccgtctac
      601 acgccgctgt tctcgggcgt gttctgggac cagcaggacc tggtgctcgc ggacgtcgac
      661 cgtatcgagg tcatcagcgg tccaggcgcc acgctgtggg gagcgaacgc cgtcaatggc
      721 gtcatcaacg tcatcactcg ttccgccgcc gaaacgcaag gcgggttcgt ctcggccggc
      781 ggcggcaatt tcgagagcca ggcgactctg cgctacggcg gccggctcgg aggctccggg
      841 cactaccgcg cgtatgcaaa gacggcgagc ttcgacgcca cgcagcgcgc gagcggcgct
      901 tcggcgctcg acgcttggac gcgccagcag gttgggttcc gcgcggattg ggacctgggc
      961 cgcgacagcg tgacgctgca agccgacgtc gcggacggcc gctcccagga tcgcggcacg
     1021 gccgcgggct ttgctttcgg ccgaatcgaa acgtccggcg cgaatgtgct cggccgcttc
     1081 aagcgcgact tgggcgacgg ctcgtcgctg agcgtgcaga gttacctcga ccacagcgag
     1141 cgcgacgacc tcttgttctt ccgcccgcgc cgcgacacgt tcgacgtcga ggcgcagcat
     1201 ggcttcgagc gcggcgccaa cgacgtcatc tggggcggcg gctatcgcca catgagcgac
     1261 gagatcggca ccggcttcat cacgaccttc attccgggca gccgcgacct cgagtgggcg
     1321 aacgtgttcg tccaagatcg catccagttg cgcgcgaatc tcgagactac cgtggggctg
     1381 aagctcgagc gcaacgacta caccggcacg gaatcgctgc cgagcgcgcg gctcgcgtgg
     1441 cgaccgagcg acgaccggct cctctggacc gccgtgtcgc gcgcggtgcg agcgccgtcg
     1501 cgggtcgatc gcgaggtgtt cttcccgggc acaccgccgt tcatcgtcgt cggtggtccg
     1561 aacttccaat ccgaggtcgc caacgtcgtc gagctcggct atcgcgcgca gccggcgccc
     1621 gggctcagct actccgctac gctgttccgt cacgactggg acaagttgcg cagcggcacg
     1681 gctgttcccg tgcagctcga gaatcgcatc gagggcgacg tctacggcgt cgaagcgtgg
     1741 gcgaattggc gcgtcacgga acgctgggag ctcagcggcg gcttgaacac gctcgacaaa
     1801 gacctcgtgc tcgagcccgg cagcacggac ccggccggcg tcaacaaccc gaccctggcc
     1861 aacgacgccg actttcagtg gacgctgcgc tcggccctgc aactgccgat cggcttgcag
     1921 ctcgatgtcg ccgtgcgacg cgtcgattcg ttgccgaacc cgcgcgtgcc gcgctacacg
     1981 gccgtcgacg cccggctcgc gtgggaccac ggcgccaatc tcacggtcga tctcaacgtg
     2041 cgcaacctgt tcgacgccaa gcacccggaa ttcgaagcgg cgccgaatcg cagcgagctc
     2101 ccgcgaaccg tgctgctgaa ggtcgactgg cggttcggcg gctgagccct ttctgacggc
     2161 tggcttccgc cgggccgccg cctttatgtc aagtcgcggc aacgcaaccg ggcgtcgcgc
     2221 gtgttggctc aggcagcgaa agccggcgaa ccggccctac gcacgtccaa caagaactaa
     2281 caatgacggc gtccagtacg gcgagcagcc tcccgcagcg cgatgcgaaa cccgcgatcg
     2341 tggcgctcgc tctccgcgac aagacgctgg cggctacgct ggcgcaacgg ctcgcggccg
     2401 gcctcggcgc cgacgtgctc cagttccggt cctcgagccc cgcgcaactc gtgccgtggc
     2461 tggacgacgc gcgcgccgac gtcctactgc tcgaccagcg ctggttgcac cgcctggccg
     2521 gcgctgcgac gcgcagactg ggtgcgcgct ggcctgcaca acgcgtgctg ctcgtgggcg
     2581 accgcgcgtg ccgggcactc gcagagcacg tcgtgcgcca acgactgcac ggtttcttgc
     2641 tcgcaaacga ggcggccgag ctctgcgcca aggccgtcgg cgtcgtcgag cgcggcgaga
     2701 tttgggtccc gcggacgctg ctcgtcgagc tgctgttcga gcacttcgat tcggcgggcg
     2761 aaggcgaggc gccgagcgac gtcaagctca cgcgccgcga aacggaagtc gtcggttacg
     2821 tgcggcgcgg cttcgcgaac aagcagatcg cggagtcgct tgcgatccgc gtcgacaccg
     2881 tgaagaagca tctgcgtagc gcgtacacga agctcggcgt gcatcgccgc agcgaaatca
     2941 tgatgggcgc cgtgcgcctc gccggcactt agccgttgca ttcaagcgcc ggccccgggg
     3001 gtttgcgaga ctttcccgcg gtccgagtcg tgagtctttg gggggccaca atgggatacg
     3061 ccgtcggtat cgcgctcgcg cttttcgtgt cgctgttcgc tcgactgacg ggcctcgatc
     3121 gcgaccgagc gttctacccg acgatcgcga tcgtcatcgc tttgctctac gtgctgtttg
     3181 ccgtgatggg cgcgtcgagc catgcgctgc tggtcgactc gattccgatg gccgcgttcg
     3241 tgctcctggc tgtgctcggc ttcaagctga acctgtggct cgtcgtcgcg tgcttggccg
     3301 gtcacggcat attcgaccta ttccatccgc acgtcatcga caatcccggc gtgcccgaat
     3361 ggtggccgag cttctgcatg acgtacgacc tcgtcgcggc cgccttcttg gccgggctct
     3421 tactgcgcgc gaaaatcgcg gtgcgcgtcg cgagccgcta gctctcagcc gctgccgcgc
     3481 cctcacgcac gtcgctgttc gccggcgcgg gggaagagcg gcgcacacga gagctcgtgt
     3541 gcgccgccgg ggccgcgggc cgcctttact cgacgccttc ggaaggaatc tggaacaacg
     3601 ggctcagcga gatgctgccg tgattgccgg gcagctgcgg gtcatcgctc gacgggacgc
     3661 cggggttcaa attcgacgcc gtgtcgtccg gggggaacag caccagcgtt tgcagaaagt
     3721 cgatgatctt ctcctgattg tcaggaccga gcgcgacgta ggcgcgcgtc acggctgcgg
     3781 cttcgccacc gtggcgccga atgacggcgt cgagcgtgac gctgcgcccg tacccgacct
     3841 attcgtagag acttctcgat gcagcccgcg ggtgcgtcga tgagccccgc tgcgttgatg
     3901 agctcgcact cgttgagtta ggcgctcgca ttctcccgcg agctgacgag gacgataggt
     3961 catgttgccc ctccatgggt gcgcggctat tcacacgagg aacgcgcgtg tgtgcgtgcg
     4021 acggggcgca gaatcccgtg ctcgccgcaa tcccgcggct cgctagcgag tgctgaatcg
     4081 gcagccttaa cgcgtcatgg ctccggtatt ggccggcatg cccccttgac gcgacgcatt
     4141 ga
//
LOCUS       DATLEE010000286         3182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_328921, whole genome shotgun sequence.
ACCESSION   DATLEE010000286 DATLEE010000000
VERSION     DATLEE010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328921"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VM692_06010"
     CDS             complement(<1..316)
                     /locus_tag="VM692_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HUQ51756.1"
                     /translation="MTDQIRFKLDGKEVAANAGETIWDVAKREGTRIPHLCHVDLPGY
                     RVDGNCRACMVEVKGERVLTASCIRKPTPGMEVSTQSERSVKSRNMVFELLASNMRPR
                     EQG"
     gene            complement(318..1994)
                     /locus_tag="VM692_06015"
     CDS             complement(318..1994)
                     /locus_tag="VM692_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HUQ51757.1"
                     /translation="MRPETPRSRRERGPKGRVVDDAALAEVRALLGTRERRRDLLIEH
                     LHLIQDKYGCLEARHLRALADEMRLSQAEVYEVASFYDHFDIVKEGEPKPAPVTIRVC
                     ESISCMIAGADQLIERLNAGVDPHQVRVVRAACIGRCAAAPAARVGDREVDNATVEGL
                     LRDVRDRRTGVVVPKYVALDEYRKNGGYELYEAVRSGKLGAEAIIETLQSSGLKGLGG
                     AGFPPGKKWQFVRSYQGPRLMSINGDEGEPGTFKDRWFLERDPHRTLEGALIAAHSVE
                     AQRIYFYMRNEYPGVLEILRREIAALEAAGIAPAGFIELRRGAGAYICGEESAMIESI
                     EGKRGLPRNRPPYIAEVGIFGRPTLNQNVETLWWIRDILQKGPEWFAKQGREGHPGPR
                     SWSVSGRVKQPGVVQAPAGVTVRELIDDYCGGMLDGHEFTAYLPGGASGGLLPAAMGD
                     IPLDFGGELAKIGAFVGSHAVVVFSNHDDVKDAVVNLLKFFRHESCGQCTPCREGTDK
                     MVTLLKTKGALNEPALRDLETVMRDSSICGLGQAAPKPVAHLLTLFRKDS"
     gene            complement(2031..>3182)
                     /gene="glp"
                     /locus_tag="VM692_06020"
     CDS             complement(2031..>3182)
                     /gene="glp"
                     /locus_tag="VM692_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HUQ51758.1"
                     /translation="SVSLAEADGRVLAADLTATLQLPPFTNSAVDGYAVRAADVSSGE
                     PRKFDVAGRLQAGSAAAGPAIAPGQAVRIFTGAPMPPGADTVFMQEDAQPDGAGGVVL
                     PAGLEKGANVRPAGEDITLGRVVVPAGRCLRPQDVAVAAAMGLTQLSVRRRVKVAVFS
                     NGDEIVEPGAARRDTQVFDSNRFMLAAMLKRIGCDVRDLGIRRDDSAAIAAVLEDAAR
                     SNDLILSTGGVSTGEGDFVKTAIERVGTLVFWRIAMKPGRPVAMGVIDGTPFIGLPGN
                     PVASFVTFARVARPAILALAGATWLPPPAVPVRAQVHYRKKTARREYVRVHLRKSEDG
                     TLEAAKFPREGAGLLSSLVDTDGVVELPEDLTRVEPGQIVDCFTYASLL"
BASE COUNT          509 a         1095 c         1043 g          535 t
ORIGIN      
        1 gcccttgctc gcgcggccgc atgttgctcg cgagcagctc gaacaccatg ttgcgcgact
       61 taacggagcg ctcggattgc gtcgagactt ccattcccgg cgtcggtttg cggatgcacg
      121 aggccgtcag cacgcgctcg cctttgactt cgaccatgca ggcgcggcaa ttgccgtcga
      181 cgcggtaacc gggcaggtcg acgtggcaca ggtgcgggat tcgcgtgcct tcgcgcttgg
      241 caacgtccca gatagtctcg cctgcgttcg cggcgacttc cttgccgtct agcttgaatc
      301 gaatttggtc cgtcatctca agagtccttg cggaacagcg tcagtagatg cgcgacgggc
      361 ttgggggccg cctgaccgag gccgcagatc gaggaatcgc gcatgacggt ttcgaggtcc
      421 cgtagggccg gctcgttcaa tgcgcctttg gtcttcagca gcgtgaccat tttatcagtg
      481 ccttcccggc agggcgtgca ctgcccgcag ctctcgtgcc tgaagaattt caaaaggttg
      541 accacggcgt ctttgacgtc gtcgtggttc gagaacacga cgacggcatg cgagccgacg
      601 aaggcgccga tcttggcgag ctcgccgccg aagtcgagcg gaatgtcgcc catggccgcc
      661 ggtaggaggc cgcccgacgc gccgcccggc agatacgccg tgaactcgtg gccgtcgagc
      721 atgccgccgc aataatcgtc gatgagctcg cgcaccgtga cgccggccgg cgcctgcacg
      781 acgccgggct gcttgacgcg gcctgacacg gaccacgagc gcgggcccgg atgcccttcg
      841 cgcccctgct tcgcgaacca ctcagggccc ttctgcagga tgtcgcggat ccaccagagc
      901 gtctcgacgt tttgattgag cgtcggccgg ccgaagatgc cgacctcggc gatgtacggc
      961 ggtcgattgc gcggcagtcc gcgcttgcct tcgatgctct cgatcatcgc gctctcttcg
     1021 ccgcagatgt acgcgcccgc gccgcggcgg agctcgatga aaccagcagg cgcaatgccg
     1081 gcggcctcga gcgcggcgat ctcgcgccgc aaaatctcga gcacgccggg atactcgtta
     1141 cgcatataga aatagatgcg ctgcgcctcg acggagtgcg cggcgatcaa cgcgccttcc
     1201 aacgtgcggt gcggatcgcg ctcgaggaac cagcggtcct tgaacgtgcc gggctcgcct
     1261 tcgtcgccgt tgatcgacat caggcgcggc ccttggtaag agcgcacgaa ctgccacttc
     1321 ttgccgggcg ggaagccggc gccgccgaga cctttgaggc ccgacgactg cagcgtctcg
     1381 atgatcgctt cggcgccgag cttgcccgag cgcacggctt cgtagagctc atacccgccg
     1441 ttcttgcggt actcgtccag cgcgacgtac ttcggcacga cgacgcccgt gcgccgatcg
     1501 cgcacgtcgc gcagcaggcc ttcgaccgtc gcgttgtcga cctcgcgatc gccgacgcgc
     1561 gcagccggcg ctgcggcgca gcggccgatg caggcggcac gcacgacccg aacctgatgc
     1621 gggtcgacgc cggcattcaa gcgctcgatc agctgatcgg cgcccgcaat catgcaactg
     1681 atcgattcac agacgcggat cgtgacgggg gccggcttcg gctcgccctc tttgacgatg
     1741 tcgaagtggt cgtagaagga agcgacctcg tagacctcgg cctgcgacaa ccgcatctcg
     1801 tcagccaacg cgcgcaggtg ccgcgcttcg aggcagccgt atttgtcctg aatcaggtgc
     1861 aggtgctcga tcaatagatc gcggcgccgc tctcgggtcc cgagcagtgc tcgaacctct
     1921 gccagggccg catcatcgac cacgcggcct ttcggcccac gctcgcggcg cgagcgcggt
     1981 gtttcaggtc tcattggaaa tccctctccc cgcgtctgaa gccggtacgg tcagagcaga
     2041 gaggcatatg taaaacaatc gactatttgt cccggctcga ctcgcgtgag gtcctctgga
     2101 agctcgacca cgccgtcggt atccaccaat gaggatagca gcccggcccc ctcgcgcgga
     2161 aatttggccg cctcgagcgt gccgtcctcg gacttgcgaa ggtggacgcg cacgtactcg
     2221 cggcgtgcgg tcttcttgcg atagtggacc tgcgcgcgca ccgggaccgc cggcggcggt
     2281 aaccacgtcg cgcccgccag cgcgaggatc gcgggccgcg cgacgcgagc gaacgtcacg
     2341 aagctggcga cggggttacc cggcaagccg atgaacggcg tgccgtcgat gacgcccatc
     2401 gcgaccggcc gcccgggctt catggcgatg cgccagaaca cgagcgtccc gacgcgctcg
     2461 atcgcggtct tgacgaagtc gccctcgccc gtcgagacgc cgccggtgct caaaatcagg
     2521 tcgttcgacc gggcggcgtc ctcgagcacc gccgcgatcg ccgcgctgtc gtcgcggcga
     2581 atgccgaggt cgcggacgtc gcaaccgata cgcttgagca tcgcggccaa catgaagcgg
     2641 ttcgagtcga agacttgcgt gtcgcgccgt gcagctcccg gctcgacgat ctcgtcgccg
     2701 ttcgagaaca cggcgacctt gacgcgcctg cgcacgctga gctgcgtgag tcccatcgcc
     2761 gcggcaaccg ccacgtcttg cggacgcagg caccggccgg ccggcacgac gactcgcccg
     2821 agcgtgatgt cctcgccggc cgggcgcacg ttggctccct tctcgaggcc ggccggcaac
     2881 acgactccgc cggcgccatc gggttgtgcg tcctcctgca tgaaaaccgt atcggcgccc
     2941 ggcggcatcg gcgcgccggt gaaaatacgg acggcttgac ccggcgcaat ggccggaccg
     3001 gcagctgcgc tgcctgcttg caagcgcccc gctacatcga actttcgtgg ctcgccgctc
     3061 gacacgtccg cggcgcgcac ggcgtagccg tcgacggccg agttcgtgaa cggcgggagc
     3121 tgtagcgtgg ccgtcagatc ggcggccagc acgcggccgt cggcctcggc cagactcacg
     3181 ct
//
LOCUS       DATLEE010000287         3687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2564735, whole genome shotgun sequence.
ACCESSION   DATLEE010000287 DATLEE010000000
VERSION     DATLEE010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564735"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VM692_06025"
     CDS             complement(<1..532)
                     /locus_tag="VM692_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HUQ51759.1"
                     /translation="MRDQTRAVARALCYVWMWSAATVLAQSGQRSAPPAAAPETATVA
                     RYEVQPSDRLQVAVWREPELTQEVVVRPDGAFSFPIVGEINAVGKSVEELRLELVQRL
                     SRIIPDVVVTVAVLEIRGNKVYVIGQVIRPGEFIVNPRVDVIQALSLAGGTTPFASPS
                     EIFVLRREGAQQRRLPF"
     gene            695..2185
                     /locus_tag="VM692_06030"
     CDS             695..2185
                     /locus_tag="VM692_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HUQ51760.1"
                     /translation="MTKNEPVGLWMLPAHVGRSRSALATLQRDFARLRTLEHPHIARM
                     LELGCNGQQYYVTGERLDGEPLREVLTHLAPEHLEVGEADEIVRAIGSALVYSHEQGV
                     SHGDVRAENVLVTMDRRFMLTNFLARRVARISARPPQPSDDLAGLARLAAELYTGSTS
                     PHAVRSAAHGSVPAARLNAIRTVLEVSPNRRPASVAQFLAAAGLAYPAATVVAVRQPT
                     VRQQRSWSLWRFVLPVGAAIGLGTLVAAYHDAGGNVRDSAVELQRRGLEALNAVASRV
                     KPPASASASEQSTASERLPASERPAAAGERASPPPTEPAPSAGAAPHATEPPATSSAA
                     AASARPTPTETVVDPPVGSLPAGQPAAAPVPPAEAAAAPAPTTTAAVRRGDPAVLSLG
                     APKVAAREDHSVVAIDVVRSGDTTHETDVGWWTSPGTAYENDDYAGGRRMVTFPAGAT
                     VERLLIPIVNDGVREPDEVFTVHLSRPRNGVTGTVTATRVTLYDDD"
     gene            complement(2188..2460)
                     /locus_tag="VM692_06035"
     CDS             complement(2188..2460)
                     /locus_tag="VM692_06035"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HUQ51761.1"
                     /translation="MQLDPSKQRELAYRLWEERGRPEGRAEEDWFEAERRLQGDQHRA
                     DSAAVDEAMRESVPASDPPSSGLPDKPPVNVKKRRPQMSRRPSADN"
     gene            complement(2556..2801)
                     /locus_tag="VM692_06040"
     CDS             complement(2556..2801)
                     /locus_tag="VM692_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51762.1"
                     /translation="MVVTATASQSAVLFLFVLSFLCFMTGAALRRWPDRVQAYMERVD
                     GSLWFVSAETHRALILATGIALTALSVLALVVARLIS"
     gene            3279..3479
                     /locus_tag="VM692_06045"
     CDS             3279..3479
                     /locus_tag="VM692_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HUQ51763.1"
                     /translation="MNWDVISGQWKQLKGKAQQKWGKLSDSDWDRIEGKREQLVGRLQ
                     ELYGKQRDDAEREVDDWGRGLH"
BASE COUNT          643 a         1237 c         1242 g          565 t
ORIGIN      
        1 tgaacggcag gcgccgctgt tgggccccct cgcggcgcag cacgaaaatc tcgctcgggc
       61 tcgcgaacgg cgtcgtgccg cccgcgaggc tgagcgcctg gatgacgtcc acgcgcgggt
      121 ttacgatgaa ctcgcccggc cggatgacct ggccgatgac gtagaccttg ttgccgcgga
      181 tctcgagcac ggcgaccgtt acgacgacgt caggaatgat gcggctcaag cgctgcacga
      241 gctcgaggcg cagctcttcg acgcttttgc ccacggcgtt gatctcgccg acgatcggga
      301 acgaaaacgc gccgtccggc cggacgacga cctcttgagt cagctcgggc tcgcgccata
      361 cggcgacctg tagccgatcg ctgggctgca cctcatagcg agcgacggtc gccgtctccg
      421 gcgctgccgc cggcggcgcg gatcgctgac ccgattgagc cagcacggtc gcggccgacc
      481 acatccacac gtagcagagc gctcgcgcta ccgcgcgcgt ctggtctctc atgcgcctac
      541 ctcccgcagt agcgctgtcg aatgtctccc cgacccagag gagcaatatc tgtgccaaac
      601 gcgcaacgcc tcggacactg gcatagacat tgcaccctcc ccgagaacgc gcgggctggc
      661 cgcgggttcg gtttcgcaac ttagggaggc aggcttgaca aagaatgagc ccgtcgggct
      721 atggatgctg cccgcgcacg tcggaagaag tcgcagcgcg ctcgcaacac tgcagcgcga
      781 cttcgcccgt ctgcgtacgc tcgagcaccc ccacatcgcg cgcatgctcg agctcggttg
      841 caacggccaa cagtattacg tgaccggcga gcgcctcgac ggcgagccac tgcgcgaagt
      901 gctgacgcac ttggcgcccg agcatctcga ggtcggcgaa gcggacgaaa ttgtccgggc
      961 catcggctcg gcgctcgtct attcacacga gcagggcgtc tcgcatggcg acgtgcgcgc
     1021 ggaaaacgtg ctggtgacga tggatcgacg gttcatgctg acgaatttcc tcgcgaggcg
     1081 cgtcgccagg atctcggcgc gcccgccgca accgagcgat gacttggcgg gtcttgcgcg
     1141 gctcgcggcc gagctctata cgggctcgac gtcaccgcat gccgtgcgca gcgccgcgca
     1201 cggcagcgtg ccggccgcac gtttgaacgc gatccgcacc gtgctcgagg tctcaccgaa
     1261 tcggcgaccg gctagcgtcg cccagtttct ggctgcggcg ggactcgcct atccggccgc
     1321 gaccgtcgtt gcggtacggc aaccgacggt gcgccagcag cgctcctggt cgctgtggcg
     1381 tttcgtgctg ccggtgggcg cggcgatcgg gctcggcacg ctcgtcgcgg cgtatcacga
     1441 cgccggcggc aacgtccggg actcggcggt ggagctgcag cggcgcggcc tcgaggcgct
     1501 gaatgccgtg gccagccgcg tgaaaccgcc ggcatcggca tcggcgagcg aacagtcgac
     1561 agcgagcgaa cggctgccgg cgagcgaacg gccggcggcc gccggcgaac gagcttcgcc
     1621 gccgccgacc gaacctgcgc cctcggccgg tgcagcgccg cacgcgacag agcctccggc
     1681 cacgagcagc gcagccgcag cctcggcgcg gccgacccca accgaaacgg tagtagaccc
     1741 acccgtcggc tcactgcccg cgggccagcc ggcggcggcg cccgtgccgc ctgccgaagc
     1801 tgccgccgca ccggcgccga cgacaaccgc ggccgtgcgg cgcggcgatc ctgccgttct
     1861 gtcactgggc gctccgaaag tcgcggcgcg cgaggaccac tccgtggtcg cgatcgacgt
     1921 cgtgcgtagc ggcgacacga cgcacgaaac ggacgtcggc tggtggacgt cgcccggcac
     1981 cgcctacgag aacgacgatt acgcgggcgg ccggcgcatg gtcacgtttc cggccggcgc
     2041 cacggtcgag cgtctactga tcccgatcgt caacgacggc gtgcgcgagc ccgacgaggt
     2101 gttcaccgtg catttgagcc ggccgcgtaa cggcgtgacg ggcactgtga ccgccacgcg
     2161 cgtgacgcta tacgacgacg actgagctca gttgtccgct gagggccggc gcgacatctg
     2221 cggccggcgc ttcttcacgt tgacgggtgg cttgtcgggc agccccgagc tcggtgggtc
     2281 gctcgcgggg accgattcgc gcatggcctc gtccacggcg gccgagtcgg ctcggtgctg
     2341 atcgccttgc aaccgtcttt cggcttcgaa ccagtcttct tcggcacgcc cttcgggtcg
     2401 gccgcgctcc tcccaaagtc tgtatgccag ctcacgttgt tttgatggat cgagttgcat
     2461 gcccacgact gtgcctccgt cgctccgagc ggttggattg atcgcccgct gcgatgcacg
     2521 gcttatgcca gcccccgctg cgaccgtccg caggactagg agattagccg ggcgacgacg
     2581 agcgcgagca ccgacagcgc ggtgagggcg atgcccgttg cgagaatcaa ggcgcggtgc
     2641 gtctcggcgc tgacgaacca gagcgagccg tcgacgcgct ccatgtaggc ctggacgcgg
     2701 tccggccagc ggcgcaatgc ggcgcccgtc atgaaacaca ggaacgatag gacgaacaag
     2761 aacaatacgg cgctttgcga ggccgtcgcg gttacgacca tagggctttg gacctctatt
     2821 tttttgccgt gcgcggccca cggcgagcgc gcgcagaata ccggagccca ccagcggccg
     2881 tcgagccgcg gcacgcagtt tctgcgatga cttcaaaaat ttgcgtgcgc gccgcaaccc
     2941 gtggcgcgga agccgcttcc ctgcccgcac tggcacagac cttgcattgt taaatgcgtg
     3001 ctccggcacg caacgcaagt tgggtaccgc agcgccgcaa gcccaggacg cctgggcttg
     3061 tttttatagg gaagtcagga ggctctatgc aagtttggag acgctcgatt gtttggcttt
     3121 tgctggccgg cgctatcgca cccctcaccg cctgtgacaa gggacctttc gaggaggctg
     3181 gcgaggagat cgacgatgcc gttgacgacg ccggagacgc gatcgatcga cgcaactgag
     3241 gtttgcacgc aaacgctcag ccaacacgga ggaaaggcat gaactgggac gtcatcagcg
     3301 gtcagtggaa gcagctcaag ggcaaagcgc agcagaagtg gggcaaattg agcgatagcg
     3361 actgggatcg catcgaaggc aaacgcgagc aactcgtcgg ccgcctgcaa gagctttacg
     3421 gcaagcagcg tgacgacgcc gagcgcgaag tcgacgactg gggtcgcggc ctccactgag
     3481 gaacgaacgt gggtacgacc ctcgcgcacg cgggtggcga cgtagcggcg ctttggcgcg
     3541 ccaccccgtg cgcgatggca tgaaatctgc agaaagcgcg caaatcccgc aacggagcgg
     3601 ctcatgacga cgaatggtca caaggagacg aaagaaacca ctaccgaccg cttcgccgcc
     3661 aaggcgcacg agacggtcga tagcctc
//
LOCUS       DATLEE010000288         2369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6126437, whole genome shotgun sequence.
ACCESSION   DATLEE010000288 DATLEE010000000
VERSION     DATLEE010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6126437"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /gene="galU"
                     /locus_tag="VM692_06050"
     CDS             complement(<1..788)
                     /gene="galU"
                     /locus_tag="VM692_06050"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HUQ51764.1"
                     /translation="MARIRTAVFPVAGRGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     ALDAGVERLVFITGSSKRAIEDHFDTDAELERQLSESGKHDMLGLVRDIVPPEATCIF
                     VRQGIPLGLGHAVLCAKAVVNDEPFFVHLADDLIYSKVGCLRQMREHFEKHGASVLGV
                     ETVPADQTGSYGIVAVQKEPSGAQRVTRIVEKPKPKDAPSNLAVVGRYILTPAIFAKL
                     ERTQRGAGGEIQLTDGIASLLADEPVHVLPFAGTRYDCGSKIGYL"
     gene            complement(913..1206)
                     /locus_tag="VM692_06055"
     CDS             complement(913..1206)
                     /locus_tag="VM692_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HUQ51765.1"
                     /translation="MKSRTLRIGYALALACGAFVANAGPVNINTADAAKLASELTGVG
                     PALAEAIVADRKTNGNFATAEALTRVKGIGARIVEMNKANILVADPPPPAGAR"
     gene            complement(1297..>2369)
                     /locus_tag="VM692_06060"
     CDS             complement(1297..>2369)
                     /locus_tag="VM692_06060"
                     /inference="COORDINATES: protein motif:HMM:NF037181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51766.1"
                     /translation="ADYIRGLNLVLSRRTDEALELFVQMAKVDDDTLETHFALGHLFR
                     RRGEIERAIRVHENLLARPNLNEAQRHQAVFSLAEDYLGAGLFDRAERLFHELRDSTT
                     LAEAALDKLVGIYEREQEWQKAIDAHRKLEMLRGSKSSQVAHYYCELAELARAAGDRA
                     LARELLRNTIRSESGALRGTLIRATLAREEQDYAQAIPLYEQVIAADRHFFTEVLPPL
                     LECYRAANRLPEFDAYVERLSAKDPTLHRDLAYAAIIGALNGSAALATAVERFVFEHN
                     VLANLVNAPELRTLPQEQRTAAIQRISEGLRTIALANARYRCSNCGYSTQRFIWHCPS
                     CKSWETVRPIQSVPLETVLGQP"
BASE COUNT          388 a          762 c          782 g          437 t
ORIGIN      
        1 aggtagccga tcttgctgcc gcagtcatag cgagtccccg cgaacggcaa cacgtgcacg
       61 ggctcgtccg cgagcaagct cgcgatgccg tccgtcagtt ggatctcgcc gcccgcgccg
      121 cgctgcgtgc gctcgagctt cgcgaaaatc gccggcgtca gaatgtagcg gccgacgact
      181 gcaaggttcg acggagcatc tttgggcttc ggcttctcga cgatgcgcgt cacgcgctga
      241 gcgccgcttg gttctttttg cacggccacg atgccgtagc tgccggtctg atccgccgga
      301 accgtttcga cgccgagcac gctggcgccg tgcttctcga agtgctcgcg catctgccgc
      361 aagcagccga ccttgctgta gatcagatcg tcggcgaggt gcacgaagaa cggctcgtcg
      421 ttgacgacag ccttcgcgca aagcacggcg tgcccaagcc cgagcggaat gccctgccgc
      481 acgaagatgc aagtggcctc gggcggaacg atgtcgcgta cgaggccgag catgtcgtgc
      541 ttgcccgact cggacagctg ccgctcgagc tccgcgtccg tatcgaagtg atcctcgatg
      601 gcgcgcttcg aggagcccgt gatgaagacc aagcgctcga cgcccgcatc gagcgcctcc
      661 tcgacggcat attgaatcag cggcttgtcg acgatcggca acatctcctt cggagaagcc
      721 ttggtggccg gaagaaaacg cgtgccgcgg ccggcaacgg ggaatactgc ggtgcgaatg
      781 cgtgccatcg acaatcctcg gtagtgtgtt ctgaaatcat acctaacgcc gcgtgatttt
      841 ttcgggcgcg tcgtgcaact accgaggcgc aacgggtgcg aggccgcctg gccccgcacc
      901 cgtgcggtgt cgtcagcggg ctcccgcggg cggcggggga tcggcgacga gaatgttggc
      961 tttgttcatc tcgacgatgc gtgcgccgat gcctttgacg cgcgtcaacg cctccgccgt
     1021 cgcgaagttg ccgttggtct tgcggtccgc gacgatcgct tcggccagtg ccggccccac
     1081 tcccgtgagc tcgctcgcca acttggccgc gtcggccgtg ttgatgttga ccgggccggc
     1141 gttcgcgacg aaggcgccgc acgccagcgc cagcgcatag ccgattcgaa gagtcctcga
     1201 tttcatggtc tcgctcctgt ggtgaaagga gcggccatgc tagtcagcgt gctgcacggc
     1261 ggcagtaaag caaattcgcc ggttcgcgca gtttcgttac ggctgcccga gcaccgtttc
     1321 gagcggcacg ctttgaatcg gccgcacggt ctcccagctc ttgcagctcg ggcagtgcca
     1381 gatgaagcgc tgcgtgctgt agccgcagtt cgagcaacga tagcgcgcat tggccagcgc
     1441 gatcgtgcgc agcccttccg agatgcgctg gattgcagca gtgcgctgct cttgcggcag
     1501 cgtgcgcagc tccggcgcat tgacgaggtt cgcaagcacg ttgtgctcga acacgaatcg
     1561 ctcgacggct gtcgcaagcg cggccgagcc gttcagcgcg ccgatgatcg cggcataggc
     1621 gagatcgcgg tgcagcgtcg ggtctttggc gctgagccgc tcgacatagg catcgaactc
     1681 gggcagccga ttcgcggcgc gatagcactc gagcagtggc ggcaatacct cggtgaagaa
     1741 gtggcgatcc gcggcgatga cctgctcgta gagcgggatc gcctgggcgt agtcttgctc
     1801 ttcgcgcgcg agcgtggcgc gaatcagcgt accgcgcaac gcgccggatt cgctgcggat
     1861 cgtgttgcgc agcaactcgc gcgcgagcgc ccggtcgccc gcggcgcgcg cgagctcagc
     1921 aagctcgcag tagtagtgag cgacctggct cgatttagac ccgcgcagca tctcgagctt
     1981 gcggtgcgcg tctatggctt tctgccactc ctgctcgcgc tcgtagatgc cgacgagctt
     2041 atcgagcgcc gcttcggcaa gcgtcgtgct gtcgcgcagc tcgtggaaca gtcgctcggc
     2101 tcggtcgaac aggccagccc ccaggtagtc ttccgccaac gagaacacgg cttggtgccg
     2161 ctgcgcctcg ttgaggttcg gccgcgccag caagttctcg tgtacgcgaa tcgcacgctc
     2221 gatctcgccg cggcggcgga acagatggcc caacgcgaaa tgcgtctcga gcgtgtcatc
     2281 gtcgactttc gccatctgca cgaacagctc gagggcctcg tccgtgcgtc ggctcaacac
     2341 aagattgagg ccgcgaatgt agtcggcgc
//
LOCUS       DATLEE010000289         4327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1822981, whole genome shotgun sequence.
ACCESSION   DATLEE010000289 DATLEE010000000
VERSION     DATLEE010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822981"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..440
                     /locus_tag="VM692_06065"
     CDS             33..440
                     /locus_tag="VM692_06065"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51767.1"
                     /translation="MRSDSRAAVLAMCSLLSVSTTAAAHHSFAAEFDAEKPVTLKGTV
                     VKWEMINPHGWITIDVVGTDGRTTPWMVETSNPNGLMRLGWTKRSLNPGDQITVEAYQ
                     AKDGSNTANAARITLADGSKVFAGSAGTPGAGQ"
     gene            437..1252
                     /locus_tag="VM692_06070"
     CDS             437..1252
                     /locus_tag="VM692_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51768.1"
                     /translation="MSAFAVRLSPATVCVLLATAVCGPAFAQAPAQPSASLPRQPNGR
                     PDLQGLWLKSAGGFQGLFIGSLDGTNFAGRGGPPPAPRFEYTPAAAAEKQDRERRGFE
                     DPEPHCHLPGVPRALDQPGGLYPVQIVQDENYVALLHEAMHDVRIIPTDNSKHPRRYW
                     AWDGDSRGRWEGDTFVVDVSNFNGRAWLDMQGNFVDENEHVLERFTLTDADTILYEAT
                     VTDPTVFVKPVEMRFTLKRVPAEQQILEYSCLEGERSLQHYTDEDGGKKRRVR"
     gene            1249..2091
                     /locus_tag="VM692_06075"
     CDS             1249..2091
                     /locus_tag="VM692_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51769.1"
                     /translation="MSTVRAVMLVCSSLLLAVIALEARAQNAAFTPARLWDGKTPDFR
                     GIWQVRGTAHVNIEGHRGEKGVAASPSIVVDPPDGKIPYKPDALAQRQKNYAARATAD
                     PSLKCYQAGVPRATYVPTPLQILQGPGGTLAIVYEENHAFRVFYPDTRPHFAAADFWM
                     GDTRYRWEGDTLVADVVALTDQMWMDGAGNFHSPDVHIVERYRMSDADTIEYEARIED
                     RAVYSRPWTLKTVLERVKRRDARIIEDECLEGPDGVRYHISPYDERNLLRSDYSRWTN
                     AAQK"
     gene            complement(2113..3126)
                     /locus_tag="VM692_06080"
     CDS             complement(2113..3126)
                     /locus_tag="VM692_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HUQ51770.1"
                     /translation="MSIVGVRAAARGALTIAVGVMAGAVLAQPQFGSPPQPRPDATLA
                     AAPAGFDVRREAVPAGVVERVEFDSKVTGGKRPASVYLPPGYSPDKKYPVLYLLHGIG
                     GNETHWPGPGAAGAILDTLIAEGKAVPMIVVMPNGRASNEPSTLFAGGRGPGAGPGAA
                     GPNAGAGANGGPTPAGPGAGAPRASGPPGGAAMAVEFTAYEAFERELIGDLIPFIEAR
                     YSVTGDRMHRALAGLSMGGGQSLNFGLNNVDTFAWVGGFSSAPNTKPPAELVSSPAKA
                     RQLELLWVSCGNEDGLFNISAGVHEQLASQNVPHKWYIDAGAHTFPVWKNDLYHFSSL
                     LFR"
     gene            3125..3532
                     /locus_tag="VM692_06085"
     CDS             3125..3532
                     /locus_tag="VM692_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HUQ51771.1"
                     /translation="MTASPRLLSHLHRSNAAASKSRARFPSGRRQRRNIIPITGGTVS
                     GRITGKVLMGGADYQILSPPATIDAHYLWQTDDGETIIVRNGGPFGGLVPTFETRVDG
                     PYDYLNTGSYLSSNPGMGQGGVALVFYESASAR"
     gene            complement(3564..3980)
                     /locus_tag="VM692_06090"
     CDS             complement(3564..3980)
                     /locus_tag="VM692_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51772.1"
                     /translation="MRVGNGVFAAMATFAAASALSGPASAHHAFSTEFDANQPITLKG
                     TITRVEWINPHAWIHLDVPRDGGVVEKWMVEAGSPNTLVRRGMTRESIPAGTEVVVFG
                     YRHRNGSNAANGRDVTLPDGRRLFITSPGTGAPAAE"
     gene            complement(3977..>4327)
                     /locus_tag="VM692_06095"
     CDS             complement(3977..>4327)
                     /locus_tag="VM692_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51773.1"
                     /translation="RQWVGDSRGHFEGDTLVVETTNQIPHRHYYGVQMLDSVMSDEFR
                     VVERFTRTGRDQIRYRATVHDPVFTTPWTVEIFMHPQPGELVEFACHEGNYGLRGILS
                     ATREEERRATEGRR"
BASE COUNT          744 a         1445 c         1443 g          695 t
ORIGIN      
        1 cgcccccggt ttgcaacgtg acgaggtgtg gcgtgagatc cgatagccgc gcggctgtgc
       61 ttgcgatgtg cagtctgctg tccgtcagca cgacggccgc tgcccatcac tcgttcgccg
      121 ccgagttcga tgcggagaaa cccgtgacac tgaagggcac ggtcgtgaaa tgggagatga
      181 tcaatcccca cggctggatc acgatcgatg tggtcgggac ggacggcagg accacgccgt
      241 ggatggtcga gacgtcgaac ccgaacggac tgatgcggct cggctggacg aagcgctcgc
      301 tgaacccggg cgatcagatc accgtcgagg cctaccaggc caaagacgga tcgaacacgg
      361 ccaacgcggc ccgcatcacg ctcgcggacg gcagcaaggt attcgccggc tcggccggca
      421 cgcccggcgc cgggcaatga gcgcgtttgc tgtgcggctt tcgccggcga ccgtctgcgt
      481 gctgctcgct acggccgtgt gcgggcccgc gttcgcgcag gccccggcgc agccgagtgc
      541 gtcgctgccg cggcaaccga atgggcgccc ggatctgcaa ggcctctggc tcaagagcgc
      601 cggcggcttc caaggcctgt tcatcggctc gctcgacggc accaatttcg cggggcgcgg
      661 cggtccgccg cccgccccgc gcttcgagta cacgcccgcg gctgccgccg agaagcagga
      721 ccgtgaacgg cgcggcttcg aggacccgga gccgcattgt cacctgcccg gcgtgccacg
      781 cgcgctcgat cagcccggcg gcctgtaccc agtgcagatc gtgcaggacg agaattacgt
      841 ggcgctgttg cacgaagcca tgcacgacgt gcgcatcatc cccacggaca acagcaagca
      901 cccgcgccgg tattgggcgt gggacggcga ctcgcgcggc cgctgggagg gcgacacgtt
      961 cgtcgtcgat gtgagcaact tcaatggccg cgcgtggctc gacatgcaag gcaatttcgt
     1021 cgacgagaac gagcacgtgc tcgagcgctt cacgctcacg gacgccgaca ccattcttta
     1081 cgaagctacc gtcacggatc ccacggtgtt cgtgaagccg gttgagatgc gcttcacgtt
     1141 gaagcgcgtg ccggccgagc agcagatcct cgagtacagc tgcctcgaag gcgagcgcag
     1201 cctgcagcac tacacggacg aggacggcgg caagaagcgg agagtcaggt gagcacggtt
     1261 cgcgcggtga tgctcgtgtg ctcctcgtta ctgttggccg tcatcgcgct cgaggcgcgc
     1321 gcgcagaatg cggcgttcac gcccgcgcgt ctgtgggacg gcaagacgcc ggactttcgc
     1381 gggatttggc aagtgcgcgg cacggcgcat gtcaacatcg aaggccaccg cggagagaag
     1441 ggcgtcgcag cgtcgccgag catcgtcgtc gatccgccgg acggcaagat tccgtacaag
     1501 cccgacgcgc ttgcccagcg gcagaaaaac tacgcggctc gagctacggc ggatccgtcg
     1561 ctgaagtgct accaagccgg cgtgccgcgt gcgacctacg tgccgactcc gctgcagatt
     1621 ctgcaggggc cgggcggcac gctggccatc gtgtacgagg agaaccatgc gtttcgcgtg
     1681 ttctatccag acacgcggcc gcacttcgct gcagccgatt tctggatggg cgacacgcgt
     1741 tatcgttggg aaggcgacac gctcgttgcc gacgtcgtgg ctctgacgga ccagatgtgg
     1801 atggacggtg ccggcaattt ccacagcccc gacgtgcaca tcgtcgagcg ctaccgcatg
     1861 agcgatgccg acacgatcga gtacgaggca cgcatcgaag accgggccgt gtactcacga
     1921 ccatggacgc tgaagacggt gctcgagcgc gtcaagcggc gcgacgcgcg gatcatcgag
     1981 gacgagtgcc tcgagggccc ggacggcgtg cgctaccaca tctcaccgta cgacgagagg
     2041 aatttgttgc ggtccgatta ctcgcgctgg acgaacgcgg cgcagaagta acgcgtcgcg
     2101 tccgctgccg tatcaacgga acagcagcga cgagaagtga tagagatcgt tcttccagac
     2161 cgggaacgtg tgagcgcccg cgtcgatgta ccacttgtgc ggaacgtttt gcgaggctaa
     2221 ttgctcgtgc acgccggcac tgatgttgaa gaggccgtcc tcgttaccgc acgacaccca
     2281 tagcagctcg agttgccgag ccttagccgg gctcgacacg agctcggccg gcggtttggt
     2341 attcggcgcc gacgagaacc cgccgaccca cgcgaacgtg tcgacgttgt tgagcccgaa
     2401 gttcagcgac tggccgcccc ccatcgacaa accggcgagg gcgcgatgca tgcggtcgcc
     2461 cgtgaccgaa tagcgcgcct cgatgaatgg gatcagatcg ccgatgagct cgcgctcgaa
     2521 cgcctcgtag gccgtgaact ccacggccat ggcggcgccg ccgggcggac cgctcgcgcg
     2581 gggcgcaccg gcaccgggcc cggccggcgt ggggccgccg ttcgcgccgg cgcccgcatt
     2641 cggacccgct gcaccggggc ccgcgccggg accacgaccg ccggcgaaca gcgtggacgg
     2701 ctcgttcgat gcgcgtccat tcggcatcac gacgatcatc ggtacggctt tgccctcggc
     2761 aatcaaggtg tcgaggatgg cgccggcagc gccggggccc ggccaatgcg tttcgttgcc
     2821 gccgatgccg tgcaacagat acagaacggg gtatttcttg tccggcgaat agccgggcgg
     2881 caggtagacc gaggcaggcc gtttgccgcc cgtgactttc gagtcgaact ccacacgctc
     2941 gacgaccccg gccggcaccg cttcgcgccg cacgtcgaac ccggccggcg ctgccgccag
     3001 cgttgcatcg ggacgcggtt gcggtggact gccgaactgc ggctgcgcca gcaccgcacc
     3061 ggccatcacg ccgacggcaa ttgtcagcgc acctcttgct gcggcccgta caccgacgat
     3121 cgacatgacg gcttcccccc ggttgttgtc gcacttgcat cgtagcaacg cggcggcatc
     3181 gaagtctcgc gcccgattcc cctctggccg acggcaaaga cgcaacatca tcccgatcac
     3241 gggcggtacc gtcagcggcc gcattaccgg caaggtgctc atgggcggcg ccgactatca
     3301 gatcctgtcg ccgcccgcga cgatcgacgc gcattacttg tggcagaccg acgacggcga
     3361 aacgatcatc gtgcgcaacg ggggcccgtt cggcgggctc gtgccgacgt tcgaaacgcg
     3421 cgtcgacggt ccctatgact acctcaacac tggctcgtat ttgagctcga acccgggcat
     3481 ggggcagggt ggcgtcgcgc tcgtgttcta cgagagcgcg agcgctcgtt gagcgcctgc
     3541 tgtctcgccg ccgtcgacgc gccctactcg gcggccggcg ccccggtgcc cggcgacgtg
     3601 atgaacagcc ggcgaccgtc gggcagagtc acgtcgcggc cattggcggc gttcgagccg
     3661 ttgcggtggc ggtaaccgaa gacgacgacc tccgtgccgg ccggaatcga ttcgcgcgtc
     3721 atgccgcggc gcacgagcgt gttggggctg ccggcttcga ccatccactt ctcgacgacg
     3781 ccgccgtcgc gcggcacgtc gaggtggatc cacgcgtgcg ggttgatcca ctccacgcgc
     3841 gtgatcgtgc ccttcagcgt gatcggctga ttcgcatcga actccgtcga gaatgcgtga
     3901 tgtgcgcttg ccggcccgga caacgccgat gccgcggcga acgtcgccat cgccgcaaaa
     3961 acaccattac cgactctcat cgacgtccct ccgtcgctcg ccgctcttcc tcgcgcgtgg
     4021 ccgacagtat gccgcgcaaa ccgtagttgc cctcgtgaca ggcgaactcg acgagctcgc
     4081 ccggctgcgg atgcatgaag atctcgacgg tccacggcgt ggtgaatacc gggtcgtgca
     4141 cagtggcgcg atagcgaatc tggtctcgcc ccgtgcgcgt gaagcgctcg acgacgcgga
     4201 actcgtcgct catcacggaa tcgagcatct gcacgccgta gtagtgccga tgcggtatct
     4261 ggttagtcgt ctcgacgacc aaggtgtcgc cttcgaagtg gccgcgcgaa tcgccaaccc
     4321 actgtcg
//
LOCUS       DATLEE010000290         3988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_333208, whole genome shotgun sequence.
ACCESSION   DATLEE010000290 DATLEE010000000
VERSION     DATLEE010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333208"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VM692_06100"
     CDS             <1..1379
                     /locus_tag="VM692_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HUQ51774.1"
                     /translation="SLAEELDEELKETDVDLGALAVEEGSPDAPVIKLIQSMFQDAVH
                     VNASDIHIEPDERCLRIRQRVDGLLQEQIIDGNRVAAALVTRLKLMCGLDIAEKRKPQ
                     DGRFSIKVEDKSLDVRLSTMPIYHGEAIVLRLLDQSSSRKSFAELGMPADIAERFTAL
                     VERSGGMVLVTGPTGSGKTTTLYAALQHVNSATTKIITAEDPIEYRLERVNQVQVNPK
                     IGLTFSAILRTALRQDPDIVLVGEMRDQETTEIGLRAAMTGHLVFSTLHTQSAVATVS
                     RLLDMGAQGYLIASALDGVLAQRLVRRVCDNCAQPTKPTVQQQSWLSQYLSPGEIEAA
                     KFVEGAGCTYCNMTGYRGRVGIYELLELDAGIAAAIRRNDLTEVERLAARARGFVPLV
                     KRALECALEGTTSIAEIMTSLAGLEEPARAESLLADVLTSVAAGVDGVAGEARAGATA
                     GTAARVTG"
     gene            1384..2613
                     /locus_tag="VM692_06105"
     CDS             1384..2613
                     /locus_tag="VM692_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUQ51775.1"
                     /translation="MPLFKYSGRTARGDAVAGSLESESAESLATHLFARGITPTDIKP
                     VTASEDAALDLWRRLGGGRPDLTDLILFSRQMYSITKAGLPLLRGLQSIAASTPNAIL
                     RETLGAVIEQLQGGRELSFAFGRFPDVFSKFYISVIKVGESSGTLETAFLRMYEYLGM
                     EKRIRDKVKSALRYPATVVAAIGIAVTIITIFVLPKFAPIFAALGDNLPWATRVLIGT
                     STFVASYWYIVAALLVAAVMGFRLYLRDAAGRYRWDKFKLDAPIVGGIVRRAALAQIC
                     RSFALTLEAGLPVIQALGTIARATGNEFLTERVLALRDGVERGEGLYRTSQTAGLFTP
                     LALQMIAIGEETGALDEMLDEVADFYEREVDYDLDNLSAAIEPLLIVAVGAMVTVLAL
                     GVFLPLWDLAAGAGGLG"
     gene            2617..3138
                     /locus_tag="VM692_06110"
     CDS             2617..3138
                     /locus_tag="VM692_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51776.1"
                     /translation="MLRRYAGGFSLLELVVVVIAVAILLGLALDRLLPLVGRAQRAAF
                     LDVQRELQSSLLLAAAERITSGEAATLPQLAAANPMALLLQPPANYLGELAEPADVPR
                     ASWYFDEQSGLLSYRVGRYTRFKAGEGPTDRIELRVAFAYQDRDGDGVFDAAGDRFDG
                     LKLTPVHSYDWPD"
     gene            3297..3746
                     /locus_tag="VM692_06115"
     CDS             3297..3746
                     /locus_tag="VM692_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HUQ51777.1"
                     /translation="MSRKIESGFTLIELVVVITILGILAAFAVPRFASLEGQARLAAT
                     QALAGSVRSGASLAHALWLAQGNPASVSVTMEGQAITIANGYPDAGTIDDTLVEYTGF
                     LLTTPGGTAVFTKATPSNTPIANCFVTYTPPAAANQAPNVVSTTGGC"
     gene            3820..>3988
                     /locus_tag="VM692_06120"
     CDS             3820..>3988
                     /locus_tag="VM692_06120"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUQ51778.1"
                     /translation="MNASFVRRAAGFTLVELVVVLVIIGIVAAFAAPRFFDDRTFLQR
                     GYYEELAAALKY"
BASE COUNT          638 a         1286 c         1374 g          690 t
ORIGIN      
        1 tctcgctggc cgaggagctc gacgaagagc tcaaggaaac cgacgtcgac ctgggtgcgt
       61 tggccgtcga ggaaggctcg cccgatgcgc ccgtcatcaa gctgattcaa tcgatgttcc
      121 aggacgccgt gcacgtcaac gcgtcggaca tccacatcga gcccgacgag cgctgcctgc
      181 gcatccggca acgcgtcgac ggcctgctgc aagagcagat catcgacggc aaccgcgtcg
      241 ccgcggcgct cgttacgcgg ctcaagctca tgtgcgggct cgacatcgcc gagaagcgca
      301 agccgcaaga cggccgcttc agcatcaagg tcgaggacaa gtcgctcgac gtgcgcttgt
      361 cgacgatgcc gatctaccac ggcgaggcga tcgtgttgcg cttgctcgat cagtcctcga
      421 gccgcaaatc gttcgccgag ctcggcatgc cggccgacat cgccgagcgc ttcacggcgc
      481 tcgtcgagcg cagcggcggc atggtgctcg tgaccggccc gacgggcagc ggcaagacga
      541 cgacgctgta tgccgcgctg cagcacgtga actcggcgac gacgaagatc atcacggccg
      601 aggacccgat cgagtaccgg ctcgagcgcg tcaaccaagt gcaggtgaac ccgaagatcg
      661 gcctcacgtt ctcggcgatc ctgcgcacgg cgctgcgtca ggaccccgac atcgtgctcg
      721 tcggcgagat gcgcgatcag gagacgaccg agatcggctt gcgcgcggcc atgacggggc
      781 acctcgtgtt ctcgacgctg catacgcaga gcgccgtggc gaccgtgagc cggctgctcg
      841 acatgggtgc gcaaggttat ttgatcgcct ccgcgctcga cggcgtgctc gcgcagcggc
      901 tcgttcgccg cgtctgcgac aactgcgcgc agcctacgaa accgaccgtg cagcagcaga
      961 gctggctgtc gcagtacttg agcccaggcg agatcgaagc tgcgaagttc gtcgaaggcg
     1021 ccgggtgcac atactgcaac atgacgggct atcgcggccg ggtcggcatc tacgagctgc
     1081 tcgagctcga cgctggcatc gccgccgcga tacgccgcaa cgatcttacc gaggtcgagc
     1141 ggctcgccgc gcgcgcgcgg ggtttcgtgc cgctcgtgaa gcgggcgctc gaatgcgcgc
     1201 tcgagggcac gacctcgatc gcggagatca tgacgtcgct cgcggggctg gaggagccgg
     1261 cgcgcgccga aagtctcctg gcagacgtgt tgacgagcgt ggctgcgggc gtggacggcg
     1321 tggccggcga agccagggcg ggcgcgacag ccggcacggc cgcgcgcgtg acgggctagc
     1381 ggcatgccgc tgttcaagta cagcggccga accgcgcgtg gcgacgccgt cgccgggtcg
     1441 ctggagtcgg agtccgccga gtcgctggcg acccacttgt tcgcgcgcgg catcacgccg
     1501 acggacatca agccggtcac cgcgtccgaa gacgcggcgc tcgatctatg gcggcggctc
     1561 ggcggcggcc ggccggatct cacggacttg atcctgttct cgcgccagat gtactcgatc
     1621 acgaaggcgg gactgccgct gctgcgcggc ttgcagagca tcgccgcgtc gacgccgaac
     1681 gccatcctgc gtgagacgtt gggcgccgtc atcgagcagc tgcagggcgg ccgcgagctg
     1741 tcgttcgcgt tcggccgctt cccggacgtc ttctcgaagt tctacatcag cgtgatcaag
     1801 gtcggcgaaa gctccgggac gctcgagacg gcgttcttgc gcatgtacga atacctcggc
     1861 atggagaagc gcatccgcga caaggtcaag tcggcgctgc gttacccggc tacggtcgtc
     1921 gcggccatcg gcatcgccgt cacgatcatc actattttcg tgctgccaaa gttcgccccg
     1981 atcttcgcgg cgctcggcga caatttgccg tgggcgacgc gcgtgctgat cggcacgtcg
     2041 accttcgtag cgagttattg gtacatcgtg gcagcgctgc tcgtcgcggc cgtcatgggg
     2101 ttcaggctct acctgcgcga cgcggccggc cgctaccgct gggacaagtt caagctcgat
     2161 gcgccgatcg tcggcggcat cgtacgccgt gcagcgctcg cgcagatctg ccggtcgttc
     2221 gcgttgacgc tcgaagccgg cttgcccgtg attcaggcgc tcggcacgat cgcacgcgcg
     2281 acgggcaacg aattcttgac ggagcgcgtg ctggcgcttc gcgacggcgt cgagcgaggt
     2341 gagggcttgt accgcacgtc gcagacggcg ggactgttca cgcccctggc gctgcagatg
     2401 atcgcgatcg gcgaggagac gggtgcgctc gacgagatgc tcgacgaagt cgccgacttc
     2461 tacgagcggg aggtcgacta cgacctcgac aatttgagcg ccgcgatcga gccgctgttg
     2521 atcgtggccg tcggcgcgat ggtgaccgtg ctcgcgctcg gcgtattcct gccgttgtgg
     2581 gatctcgccg ccggcgcggg cgggctgggc tgacgcatgc tgcggcgcta cgcgggcggt
     2641 ttctcgctgc tcgagctcgt ggtcgtggtg atcgccgtcg cgatcctctt aggtctagcg
     2701 ctcgatcgac tgttgccgct cgtaggccgt gcgcagcgcg cggcgtttct cgacgtgcag
     2761 cgcgagctgc agagctcgtt gctgctcgcg gcggccgagc gcatcacgag cggcgaagct
     2821 gcgacgctgc cgcagctcgc ggcggcgaat ccgatggcgc tgctgctgca gccgccggcg
     2881 aactacttgg gcgagctcgc ggagccggcc gacgtgccgc gcgcgagctg gtacttcgac
     2941 gagcagagcg gcttgctgtc gtaccgcgtc ggccgctaca cgcgtttcaa ggcaggcgag
     3001 gggccgaccg atcgcatcga gttgcgtgtc gcgttcgcgt atcaggatcg tgacggcgat
     3061 ggcgtgttcg acgctgccgg cgaccgtttc gacggtttga agctcacgcc cgtgcatagc
     3121 tacgactggc cggactgaaa gccggttgac ttaattgcgt cctgagagag tcgtcacggc
     3181 acggcgcaac gggggtgtgg tgccggtcac atcgggggcg caccgggtta actaaagtta
     3241 cggccctgag tcgtcgatac agtgccagcg ccactattca tcggaggagc gccgcagtga
     3301 gcagaaagat tgaaagcggt ttcacgttga tcgagctcgt ggtcgtgatc acgatcctcg
     3361 gcattctcgc cgcgtttgcg gtgccccgtt tcgcttcgct tgaaggacag gcacggcttg
     3421 cggcgacgca agcgcttgcc ggcagcgtgc gctccggtgc gtcgttggcc cacgcgttgt
     3481 ggcttgcgca gggcaacccc gcaagcgtca gcgtcacgat ggaaggccag gcgatcacga
     3541 tcgccaacgg ttatcccgat gccggcacga tcgacgatac gctcgtcgag tacacgggtt
     3601 tcttgctgac gacgccgggc ggcaccgctg tgttcacgaa ggctacgccg tcgaacacgc
     3661 cgatcgccaa ctgcttcgtc acctacacgc cgccggcggc cgccaaccag gcgccgaacg
     3721 tcgtgtcgac gacgggtggc tgctagcggc cgattgcttg ggaagctaat tccaacgagg
     3781 gcgcctgagg gcgccctcgt catcttcggt agacgggaaa tgaatgcatc gttcgtcaga
     3841 cgcgctgccg ggttcacgct cgtcgagctc gtcgtcgtgc tcgtgatcat aggcatcgtc
     3901 gcggcgttcg ccgcgccgcg gttcttcgac gaccgcacct tcctgcaacg cggctactac
     3961 gaggagctcg ccgccgcgct gaaatact
//
LOCUS       DATLEE010000291         2734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2570235, whole genome shotgun sequence.
ACCESSION   DATLEE010000291 DATLEE010000000
VERSION     DATLEE010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2570235"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..957)
                     /locus_tag="VM692_06125"
     CDS             complement(1..957)
                     /locus_tag="VM692_06125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ51779.1"
                     /translation="MKPKVSVVVPTHGRPELLSRCLAALAAQTLPRRDYEIVIVGDGP
                     DERTRAAVESSPCGARYVELPHQRGPAAARNAGWRAATGEIVAFTDDDTVPAPDWLMA
                     GLAAMSAGCDAVAGRVVMPLAAEPTDYERDSARLADAEFVTANCFVRAAQLRAIGGFD
                     ERFTLAWREDSDLQFELLASGAVVRRAPLAVVVHPVRPARWGVSLRQQRKVLFDALLY
                     KKHRAQYRRRIRRTPRFDYYAVVAALGTAVTAFALGAAQVAAAAAVVWLALTARLCLQ
                     RLAGTRRQWRHVGEMLVTSAVIPPLAVFWRVVGAIRFRVVLL"
     gene            complement(954..2678)
                     /locus_tag="VM692_06130"
     CDS             complement(954..2678)
                     /locus_tag="VM692_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HUQ51780.1"
                     /translation="MLTLGINAAYHDSAACLVRDGDVVAAAEEERFTRIKHGKRPVPF
                     SAWELPYHAIDYCLAEGELELADLDHVAYSFDPYLLLDRHAATMTLPLEPSAGAAAGL
                     APWEPLFVSYIINAERQLIDGAPHRLAARFRTAKERRRWQWHFVEHHLAHEASAFLPA
                     PFADAAVLTLDGRGEKTATSYGHFEGGEYRRLKQIPLPSSLGLLYEQVTDYLGFLRSS
                     DEYKVMALASYGKPSYVDWFRDIVELGADGGYVVHPARLEQRFGPKRARGAPLTQQHF
                     DIAHSLQAVLEETVIELARWLRRATGSANLCMAGGVALNCVLNGRLRDQHVFERIFVQ
                     PAAGDAGTALGAALWVDWQQGDRARRFNMEHAYLGPEFSADEIEAELKRSKVKYRRLH
                     DVADEAAALLAEDRILGWFQGRMEFGPRALGARSILASPRSADMQQRLNEIKDREDFR
                     PVAPAVLEEAAADWFVGAERAPFMLFVFDVLPEKAHLIPAVRHVDGTARVQTVGAHEQ
                     PLYHELLQAFARRTGVPVLVNTSFNSRGDPVVCTPRDALESFWSTPLDALVIGPFLIE
                     KPLFESQT"
BASE COUNT          448 a          955 c          952 g          379 t
ORIGIN      
        1 tcataacagc accacgcgga agcgaatggc gccgacgacg cgccagaaca ccgcgagcgg
       61 aggaatcacg gccgacgtca cgagcatctc gccgacatgc cgccactggc gccgcgtgcc
      121 ggcaaggcgc tgcaggcaga gccgcgccgt cagcgccagc cagacgacgg cggccgcggc
      181 cgcgacctgc gcggcgccga gcgcgaaggc cgtcacggcc gtgccgagcg cggccacgac
      241 ggcgtagtaa tcgaagcgcg gcgtgcgccg gatccggcgg cgatactgag cccgatgctt
      301 cttgtacagc agcgcgtcga acagcacctt gcgttgctgc cgcaggctca cgccccagcg
      361 cgccggacgc acggggtgga cgactacggc tagcggcgcg cgccgcacga ccgcgccgct
      421 cgcgagcagc tcgaattgca ggtccgagtc ctcgcgccac gcaagcgtga agcgctcgtc
      481 gaagccgccg atcgcgcgca gctgcgctgc ccgcacgaag caattggccg tcacgaactc
      541 ggcatcggcg agccgcgcag aatccctctc gtaatccgtc ggttcggcgg cgagcggcat
      601 cacgacacgg cccgcgaccg cgtcgcagcc cgcactcatg gcggcaagcc cggccatgag
      661 ccaatcgggc gccggtaccg tgtcgtcgtc ggtgaacgcg acgatctcgc cggtcgcggc
      721 gcgccagccg gcgttgcgcg cggccgccgg cccgcgttgg tgcggcagct cgacgtagcg
      781 ggcgccgcac gggctcgact cgaccgcagc gcgcgtgcgc tcgtcggggc cgtcgccgac
      841 gatgacgatc tcgtagtcgc ggcgcggcaa ggtctgagcc gcgagcgctg cgagacaccg
      901 gctaagcagc tccggccggc catgagtcgg cacgacaacg gacacctttg gcttcatgtc
      961 tggctctcga acaagggttt ttcgatgaga aacgggccga tgaccaatgc gtcgagcggt
     1021 gtgctccaga agctttcgag cgcatcgcgc ggcgtgcaga cgacggggtc gcctcggctg
     1081 ttgaacgacg tgttcacgag cacgggcacg ccggttcgcc gcgcgaatgc ctgcagcagc
     1141 tcgtgataga gcggctgctc gtgtgcgccg acggtttgca cgcgcgccgt gccgtcgacg
     1201 tgccgcacgg ccggaatcaa atgcgctttc tccggtagga cgtcgaacac gaacagcatg
     1261 aatggcgcgc gctccgcgcc gacgaaccaa tcggccgcgg cttcctcgag cacggccggc
     1321 gcaacgggcc ggaaatcctc gcgatccttg atctcgttca agcgctgctg catgtcagcg
     1381 ctgcgcggcg aagccaggat cgagcgcgcg ccgagcgcgc gcggcccgaa ttccatgcga
     1441 ccctgaaacc agccgaggat gcgatcttcg gcgagcagcg cggcagcctc gtcggccacg
     1501 tcgtgcagcc gccgatattt gaccttcgag cgcttgagct ccgcttcgat ctcgtcggcg
     1561 ctgaactcgg gcccgaggta cgcgtgctcc atgttgaaac gccgcgcgcg atcgccttgc
     1621 tgccagtcga cccagagtgc cgcgccgagc gcagtgccgg catcgccggc ggccggctgc
     1681 acgaagattc gctcgaagac gtgctggtcg cgcaggcggc cgttcaacac gcagttcagc
     1741 gcgacgccgc cggccatgca gagattcgcc gagcccgtgg cgcggcgcag ccagcgcgcg
     1801 agctcgatga cggtctcctc gagcacggct tgcagcgaat gcgcgatgtc gaagtgttgc
     1861 tgcgtcagcg gcgcgccgcg cgcgcgcttc gggccgaagc gctgctcgag gcgcgcggga
     1921 tggacgacgt agccgccgtc cgcgccgagc tcgacgatgt cgcggaacca gtcgacgtag
     1981 ctcggcttgc cgtacgacgc gagcgccatg actttgtatt cgtccgacga ccgcaagaaa
     2041 ccgaggtagt ccgtgacctg ctcatagagc aagccaagcg agctcggcaa cgggatctgc
     2101 ttcaagcgcc gatactcgcc gccctcgaag tgcccgtagc tcgttgccgt cttctcgccg
     2161 cggccgtcga gcgtcagcac ggccgcgtcg gcgaacggcg cgggcaggaa cgcgctggcc
     2221 tcgtgggcca ggtgatgctc gacgaagtgc cactgccacc ggcgccgctc cttcgccgtg
     2281 cgaaagcggg cggcgagccg gtgtggcgca ccgtcgatca gctgccgctc ggcgttgatg
     2341 atgtaggaga cgaacagcgg ctcccacggc gcgaggcccg cggccgcgcc ggcgctcggc
     2401 tcgagcggca acgtcatcgt cgcggcgtgc cgatcgagca gcagataggg atcgaacgag
     2461 taagcgacgt gatcgaggtc ggcaagctcg agctcgcctt cggcgagaca gtagtcgatc
     2521 gcgtgatacg gcagctccca ggcgctgaag ggcacgggcc gcttaccgtg cttgatgcgc
     2581 gtgaaccgct cctcctccgc cgccgcgacg acgtcgccgt cgcggacgag gcacgcagcg
     2641 gaatcgtgat acgcagcatt gatgcccagc gtcagcatgc gccctcgcct acgacgcccg
     2701 cacgccgggc gcggcgcggc gctcgagcga gacc
//
LOCUS       DATLEE010000292         2655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6129264, whole genome shotgun sequence.
ACCESSION   DATLEE010000292 DATLEE010000000
VERSION     DATLEE010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6129264"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VM692_06135"
     CDS             complement(<1..1232)
                     /locus_tag="VM692_06135"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017411642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HUQ51781.1"
                     /translation="MIWLVLLIGALLTLAYRRTDLRTSSIVLGALLVAYWLFGDGGFI
                     WNTLLLVLFALFAVLNVETIRRDNVTRRLFGIYRGMLPTMSKTEQEALEAGNVWWDGE
                     LFTGMPDWRRLKSLPPPKLTQEEQAFLDGPTEELCKLLDDWQTTHELADMPANVWQFI
                     KDQKFFAMIIPKEYGGLQFSPLANSMILAKLASRSAVASSTVGVPNSLGPAELLLHYG
                     TDEQKQRWLPRLASGDEIPCFALTSPRVGSDATALVDNGVVCKGTWQGEEIVGIRLNW
                     DKRYITLAPVATVMGLAFKLYDPEHLIGDVDEYGITAALIPTNLPGIEIGRRHFPINI
                     PFQNGPIRGRDVFVPLDAIIGGTKMAGLGWRMLVQQLAVGRSIVLPSNALGGASAAVY
                     ATGAYAKLRRQFNLPIGK"
     gene            complement(1286..2275)
                     /locus_tag="VM692_06140"
     CDS             complement(1286..2275)
                     /locus_tag="VM692_06140"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HUQ51782.1"
                     /translation="MKQKSKTPVRVAITGAAGQIGYQLAFRIASGQLFGPDQPIILQL
                     LEITPALNALNGVAMELDDCAFDTLHGVVVTDKADVAFKDINYGLLVGARPRGPGMER
                     KDLLLANAQIFSAQGKALDAVADRRVKILVVGNPANTNALIARENAKGLDARNFTAMT
                     RLDHNRAKAQLAGKVGGHVSDVRGVIIWGNHSATQFPDLAHATVGGAAALSKVSQGWY
                     REEFIPTVQQRGAAIIKARGASSAASAASAAIDHVRDWALGTAGDDWVSMAVSSDGSY
                     GIKEGIVYSYPVRCVRGEYQIVQGLSVDDFGRGKMAATETELREERAGVENLL"
     gene            complement(2383..>2655)
                     /locus_tag="VM692_06145"
     CDS             complement(2383..>2655)
                     /locus_tag="VM692_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUQ51783.1"
                     /translation="PYSLSPVRQELAAERPQASVVPIAAVADHGVPTDFRAAVADFEK
                     QVLRRALERAQFKQTVAAKVLGLSYHQFRSLLRKHGLVKQGAENGD"
BASE COUNT          462 a          880 c          847 g          466 t
ORIGIN      
        1 aacttgccga tcggcaagtt gaactggcgg cgcagtttcg cgtacgcgcc cgtcgcgtag
       61 acggcagccg acgccccgcc gagcgcgttg ctcggcagca cgatgctgcg gccgacggca
      121 agttgctgca cgagcatgcg ccaaccgagg ccggccatct tcgtgccgcc gatgatcgcg
      181 tcgaggggca cgaacacgtc gcggccgcgg atggggccgt tttggaacgg gatgttgatc
      241 gggaaatggc gccggccgat ctcgatgccg ggcaggttcg tcggaatcaa cgcggccgtg
      301 atgccgtact cgtcgacgtc gccgatcaga tgctcgggat cgtagagctt gaacgcgagc
      361 cccatcacgg tcgcgaccgg tgccaacgtg atgtagcgct tgtcccagtt caaacgaatg
      421 ccgacgatct cttcgccttg ccaagtaccc ttgcagacga ccccgttgtc gacgagagcc
      481 gtcgcgtcgg agccgacgcg cggcgacgtc agcgcgaagc acgggatctc gtcgccgctc
      541 gccagccgcg gcagccagcg ctgcttctgc tcatccgtgc cgtagtgcag caacagctcg
      601 gcgggcccga gcgaattcgg cactccgacc gtcgacgacg ccacggcgct gcgcgacgcc
      661 agctttgcga ggatcatcga gttcgcgagc ggcgagaact gcaacccgcc gtactctttg
      721 gggatgatca tcgcgaagaa cttttggtcc ttgatgaact gccacacgtt cgccggcatg
      781 tcggcgagct cgtgcgtcgt ttgccaatcg tcgagcagct tgcacagctc ctcggtcggc
      841 ccgtcgagaa atgcctgctc ctcttgtgtg agcttcggcg gcgggaggct cttcaggcgg
      901 cgccaatcgg gcatgcccgt aaacagctcg ccgtcccacc agacgttgcc cgcttcgagc
      961 gcttcctgct cggtcttcga catcgtcggc agcatgccgc ggtagatccc gaacaatcgc
     1021 cgcgtgacgt tgtcgcgccg aatcgtctcg acgttcaaaa ccgcgaacag cgcgaatagc
     1081 acgagtagca gcgtgttcca gatgaagccg ccgtcgccga atagccaata ggcgacgagc
     1141 agcgcgccga gcacgatgct cgacgtgcgc aggtccgtgc gccgataggc gagagtcagc
     1201 aacgctccga tcaggagcac gagccaaatc acgaatccca tcgcggctcc tcttgcgtgt
     1261 actgcggcgc cgcggcggtc gggcgctaga gcaggttctc gactccggcg cgttcttcac
     1321 gcagctcggt ttccgtcgcg gccatcttgc cgcggccgaa gtcgtcgacg gacaggccct
     1381 gcacgatctg gtactcgccg cgcacgcagc gcaccggata cgagtacacg atgccttcct
     1441 tgatgccgta actgccgtcg gacgagacgg ccatgctgac ccaatcgtcg ccggccgtgc
     1501 cgagcgccca gtcgcggacg tggtcgatgg ccgcagaggc agccgaggcg gccgaggaag
     1561 caccgcgcgc tttgatgatc gctgcaccgc gctgctgcac cgtcgggatg aactcttcgc
     1621 gataccagcc ttgcgagacc ttcgacagcg ccgccgcgcc gccgaccgtg gcatgcgcga
     1681 ggtccgggaa ctgcgtggca gagtgattgc cccagatgat cacgccgcgc acgtcgctga
     1741 catgaccgcc gaccttgccg gcgagctgcg ctttcgcgcg attgtggtcg agccgcgtca
     1801 tggcggtgaa gttgcgcgcg tcgagcccct tggcgttctc gcgcgcgatc agcgcattcg
     1861 tgttcgccgg attgccgacg acgaggatct tcacacgccg atcggcgacg gcgtcgagcg
     1921 ccttaccttg agccgaaaag atctgggcgt tggccagcaa taggtccttg cgttccatgc
     1981 cggggccgcg cggccgcgcg ccgaccagca agccgtagtt gatgtccttg aaggcaacgt
     2041 cggctttgtc cgtgacgacg acaccgtgca gcgtatcgaa cgcgcagtcg tcgagctcca
     2101 tcgccacgcc gttcagcgca ttgagcgccg gcgtgatctc gagcagctgc aagatgatcg
     2161 gctgatccgg tccgaacaat tgtcccgatg cgatgcgaaa tgccaactga tacccgattt
     2221 gacccgcggc gcccgtgatg gcgacccgaa cgggcgtctt gctcttctgc ttcatggatg
     2281 acgtttgcct cgacaaaata ctgtccaaaa ccgaattcgg gccgaaatct tagcatgcga
     2341 gcacgcgatg gcaggcgctg acgccgagtg ccgacggtcg gctcaatcgc cgttctcggc
     2401 gccctgtttc acgagcccgt gcttgcgcag caaactgcga aactggtgat agctcaagcc
     2461 caacaccttc gctgcgaccg tttgcttgaa ctgcgcgcgc tcgagagcgc gccgcaaaac
     2521 ttgcttctcg aagtccgcga cggcggcgcg gaaatccgtg ggcactccat ggtcggccac
     2581 cgcggcgatc ggcaccaccg aagcttgcgg ccgctcggcg gccagctctt ggcgcaccgg
     2641 cgacaagctg tacgg
//
LOCUS       DATLEE010000293         7233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8364317, whole genome shotgun sequence.
ACCESSION   DATLEE010000293 DATLEE010000000
VERSION     DATLEE010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8364317"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VM692_06150"
     CDS             <1..815
                     /locus_tag="VM692_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012410.4"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HUQ51784.1"
                     /translation="LERLAEDKDKYAKFWPEFGALLKEGLVEDSGNRDRLAKLMRFST
                     TKSAGSDQVRSLAEYVGDAKADQQAIYYLIAESPTAARNSPHLEAFNERGIEVLLLTD
                     RLDEWVMQHLTEYEGKPCRDVRRGALELPASDGAEQKAPAVDREQQQLLKRVKQVLRE
                     RVDEVRVGGRLRQSAACLVLNEHDLGYQMRELLAAAGHQAPAAVPSLELNLQHDLVRR
                     LDRETDPARFERLALLLFEQAVLAEGRQLDDPAAFVARLNQLLTDLGAQSGS"
     gene            812..1474
                     /locus_tag="VM692_06155"
     CDS             812..1474
                     /locus_tag="VM692_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUQ51785.1"
                     /translation="MTQSGQRTAHSTAAEIAGPAGVLEAALDSSARPAVATAVVCHPH
                     PLQDGTMRNKVVTTLSRAFTRLGADTVRFNFRGVGASAGSYADGIGERDDALAVVAWC
                     RARWPQRRVYLGGFSFGGAVAAAIAARAAPAGLVTVAPAVDRLARDFAAPSCPWLLVH
                     GDADDVVPPQPVVDWAATLATPPRVVLLPGVGHFFHGRLPALIDAVTDFFGADFAAAG
                     GG"
     gene            1476..1931
                     /locus_tag="VM692_06160"
     CDS             1476..1931
                     /locus_tag="VM692_06160"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HUQ51786.1"
                     /translation="MLRKLTALFDHAFARDNDDPQAREHGLRVATALLLIEVARADYA
                     DDSAEDATMLASLKQFFALDDAAAALLLEEARSTADRAVELQQFTRRLHEQLTVSEKQ
                     RIVEMLWQVALADHRLDKHEDHLVRRLADLLYVSHSDVIRIRNRVRGPD"
     gene            complement(1939..2436)
                     /locus_tag="VM692_06165"
     CDS             complement(1939..2436)
                     /locus_tag="VM692_06165"
                     /inference="COORDINATES: protein motif:HMM:NF025822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HUQ51787.1"
                     /translation="MVRRICMLACLILTTGLTACEPTNGGGTTSAQEGVLPATESSKD
                     FGEYVLYFNALNTDQLAPDIAREYGIVRSKSRALLNVSIHRKLDGGRTEAVTGAVSAS
                     AINLNGQLMTMTLREIREDTAIYYIGELAITNGEVLIYTIDATPSNDPSRFTVRFKKQ
                     FFVEE"
     gene            complement(2512..3057)
                     /locus_tag="VM692_06170"
     CDS             complement(2512..3057)
                     /locus_tag="VM692_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HUQ51788.1"
                     /translation="MQSALIFLIKTLADLYLLTFLLRFIMQWARASYDNPFAQFVLRV
                     TSPLVVPARRLLPSVGGLDTPTLAVLIVLEIVVTFVLLFVAGLSLPVPVMLLYALLRL
                     IALALWFYTVALFIYVLLSWFGDRGGNPMSELLGDLIEPLLRPARRLLPPIGGFDLSP
                     LIVILLLQAALIALPLPGYLR"
     gene            complement(3103..3777)
                     /locus_tag="VM692_06175"
     CDS             complement(3103..3777)
                     /locus_tag="VM692_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HUQ51789.1"
                     /translation="MTEITDHLADIVERVARAAQHAQRSATSVTIVAISKQQPSSAIV
                     EAHRVGITHFGESYVQEALPKMDAVGELAVTWHFVGKLQANKTRHVAERFAWVHTVDR
                     LRIAERLNEQRPAHAAPLNVLIQVNQGDEAQKSGAAPADVAELARAIVELPRLKLRGL
                     MTLPPQNAAAQCFAELAALRAQLVHAGIELDTLSMGMSADFREAIAAGATMVRIGTAI
                     FGRRSG"
     gene            3860..4903
                     /locus_tag="VM692_06180"
     CDS             3860..4903
                     /locus_tag="VM692_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008483697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HUQ51790.1"
                     /translation="MAAVDIAQLLAFAVKNNASDLHLSSGVPPIIRVDGDVKRINMPP
                     LKHKEVHSMVYDIMNDKQRKDFEEFLETDFSFEIPKLARFRVNAFNQNRGCAAVFRTI
                     PSVILSLDDLGAPRIFKDISMHPRGLVLVTGPTGSGKSTTLAAMVDYVNDNKPDHIIT
                     IEDPIEFVHESKKSLINQRELHRDTHGFSEALRSALREDPDVILVGEMRDLETIRLAL
                     TAAETGHLVFGTLHTSSAAKTIDRVVDVFPAAEKEMVRSMLSESLRAVISQTLLKRVG
                     GGRIAAHEIMIGTPAIRNLIREGKIAQMYSAIQTGQAQGMQTLDQNLQELMMKGLITK
                     EEARFKAVNKESF"
     gene            4952..6109
                     /locus_tag="VM692_06185"
     CDS             4952..6109
                     /locus_tag="VM692_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HUQ51791.1"
                     /translation="MERDQAVRLTQNLLKKMVENNGSDLFITADFPPAIKIDGTIRPF
                     ADRPLTSAQSSLIVRSIMNDKQAKEFDATKECNFAIAPQGIGRFRVSAFVQQGHVGAV
                     IRTIVTKIPSFEDLDLPNQLKDVAMTKRGLCLVVGGTGSGKSTTLAAMIGHRNENSRG
                     HIITIEDPVEYVHPHRGCVVTHREVGVDTDNWYNALKNTLRQAPDVIMIGEIRDSDTM
                     NYAIQFAETGHLCVATLHANSANQALDRIINFFPEERRHQLLMDLSLNITAMISQRLL
                     PRENKMGRIAAMEIMLASPLISDLIFRGEVAMIKDIMAKSNRLGMQTFDQALFQLYED
                     GAISYEEAMRNADSKNELRLKIKLQSKREGASAAEHGESLQLADEEKAGRF"
     gene            6137..7192
                     /locus_tag="VM692_06190"
     CDS             6137..7192
                     /locus_tag="VM692_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HUQ51792.1"
                     /translation="MNVKPLFKLMVDKKASDLFFTTYAPVMIKIDGKIMPVNQVELTP
                     KMVRQAALELMNEQQLEQFTRDLELDFAISEPGLGRFRVNVFHQRGNVAMVLRFVTAE
                     LPQLDALGMPAILKDMIMLRRGLILMVGASGSGKSTTLAAMIDYRNKSSASHIVTIED
                     PIEFLHSNQKSIVNQREVGLDTLSYHRALRSAMREAPDVILIGEIRDRETMQAAVDLA
                     GTGHLAIATLHANNSPETLDRIINMYPDDQHKQVLMDLSQYIRVIISQRLVRSKEGRR
                     VAAVEVMLNTPHISELILKGDISHVKEAFKDTKEPGMQSFDQALFDLYRKGAISMEEA
                     LSNADSRANLEAKINFG"
BASE COUNT         1409 a         2271 c         2304 g         1249 t
ORIGIN      
        1 tgctcgagcg gctcgcagag gacaaggaca agtacgcgaa gttttggccc gagttcggcg
       61 cgctgctgaa agaagggctc gtcgaggact ccggcaatcg cgatcggctt gcgaagctca
      121 tgcgcttcag cacgacgaag agcgcgggca gcgatcaagt gcggagcctc gccgagtacg
      181 tcggcgacgc caaggcagac cagcaggcga tctactattt gatcgccgag tcgccgacgg
      241 ccgctcgcaa cagcccgcac ttggaagcgt tcaacgagcg cggcatcgag gtgctattgc
      301 tgacggaccg gctcgacgag tgggtcatgc agcacctgac ggagtacgaa ggcaagccgt
      361 gccgtgacgt acgccgcggc gcgctcgaat tgccggccag cgacggcgcc gaacaaaagg
      421 cgccggccgt cgaccgcgag cagcagcagc tgttgaagcg cgtgaagcaa gtgctgcgcg
      481 agcgtgtcga cgaggtgcga gtcggcggcc ggttgcggca atcggcggcc tgcctcgtgc
      541 tgaacgagca cgacttggga tatcaaatgc gcgagctgct ggcggcggcg gggcatcaag
      601 cacctgcggc cgtcccgagc ctcgagctca acttgcagca cgacctcgtg cgtcgtctgg
      661 atcgtgaaac ggatcctgcg cgtttcgagc gcttggcatt gctgctgttc gaacaagccg
      721 tattggcgga agggcggcag ctcgacgacc cagcggcatt cgtcgcccgg ctcaaccagc
      781 tgctgaccga tctcggcgca cagagcggct cgtgacgcag agcggtcaac ggacggctca
      841 cagcacggcc gccgaaattg cggggccggc cggcgtcctc gaggcggcgc tcgactcgag
      901 cgctcggccc gcggttgcga cagccgtcgt ctgccatccg catccgctgc aggacggcac
      961 gatgaggaac aaagtcgtca cgacgctctc gcgtgcgttc acgcgcctcg gcgccgacac
     1021 cgtgcgtttc aacttccgcg gcgtcggtgc gtcggccggc agctacgcgg acggcatcgg
     1081 cgagcgcgac gatgcgctcg cggtcgtggc gtggtgtcga gcgcgctggc cgcagcgacg
     1141 cgtctacctc ggcggctttt cgttcggtgg cgccgtcgcg gccgcgatcg ccgcgcgcgc
     1201 cgcgccggcc ggcctcgtga ccgtcgcgcc cgcggtcgat cggctcgcgc gcgacttcgc
     1261 cgcgccgagt tgcccatggc tgctcgtgca cggtgatgcc gacgatgtgg tcccgccgca
     1321 gcccgtcgtc gactgggcgg cgacgctcgc gactccgccg cgggtcgtgc tgttgcccgg
     1381 cgtcggccac ttcttccatg gccgactgcc ggcgctgatc gacgccgtca cggacttctt
     1441 cggtgccgac ttcgccgccg ctggtggcgg ctgacatgct gcgcaagctc acggcgttgt
     1501 tcgaccatgc gttcgcgcgc gacaacgacg acccgcaggc gcgcgagcac gggttacggg
     1561 ttgcgacggc gctgctactg atcgaggtgg cgcgggccga ttacgccgac gactccgccg
     1621 aagacgcgac gatgctcgcg tcgctgaagc aatttttcgc gctcgacgac gctgccgcgg
     1681 cgctgctgct cgaggaggcg cgcagcacgg ccgatcgcgc ggtcgagctg cagcagttca
     1741 cgcgccgctt gcacgagcag ctgacggtca gcgagaagca gcgcatcgtc gaaatgttgt
     1801 ggcaagtggc gctcgcggac caccggctgg acaaacacga ggatcacctc gtgcgcaggc
     1861 tcgccgacct tctatacgtc tcgcattcgg acgtgatccg catccgcaac cgcgtgcgcg
     1921 gcccggactg agcgagcgct attcttcgac gaaaaattgc ttcttgaacc ggaccgtgaa
     1981 gcggctcggg tcgttactcg gcgtggcgtc aatcgtgtag atgagcacct cgccgttcgt
     2041 gatggcgagc tcgccgatgt agtagatggc cgtgtcctca cggatctcgc gcagcgtcat
     2101 cgtcatgagc tggccgttca aattgatcgc agacgcggag acggcgcccg tgaccgcctc
     2161 ggttcggccg ccgtcgagct tgcgatggat gctgacgttc agcagtgcgc ggctcttgct
     2221 gcgaacgata ccgtactcac gcgcgatgtc cggcgcgagc tggtcggtgt tcaaggcgtt
     2281 gaagtagaga acgtattcgc cgaagtcttt cgagctctcg gtcgcgggta ggacgccttc
     2341 ctgcgcgctg gtcgtgccgc cgccgttcgt cggctcacaa gcggtgagcc ccgtcgtcag
     2401 aatgaggcaa gccagcatgc agatgcgtct aaccatgctc cctcccggat gaccgcgcgc
     2461 tgttgttttt ggcggtaccg gcccgacatt acgcgcgtgc acgaggactg tctagcgaag
     2521 gtagccgggc agcgggagcg caatcagggc cgcttgcagc aacaagatta cgatgagcgg
     2581 cgacaagtcg aagccgccga tcggcggcaa caagcgacgc gccggacgca acaacggctc
     2641 gatgagatcg ccgagcagct ccgacatcgg attgccgcca cgatcgccga accagctcaa
     2701 gagaacataa atgaagagcg cgacggtata aaaccacaac gcaagcgcga tgagccggag
     2761 caacgcataa agaagcatga ccggcaccgg caacgacaag ccggccacga atagcaggac
     2821 gaacgtgacg acgatctcta gcacgatcaa cacggcgagc gtaggcgtat cgagaccgcc
     2881 gacgctgggc agcaagcgcc gcgccggcac gacgagcggg ctcgtcacgc gcagcacgaa
     2941 ctgcgcgaac ggattgtcgt agctcgcgcg tgcccattgc atgatgaatc gcagcaggaa
     3001 cgtcagcagg tagagatccg ccagcgtctt gatgagaaaa ataagtgcgc tctgcatcgg
     3061 ctctcgctgc tccgtcgatg gcgcgaagat agcacgacgc ggctagccgc tgcgccggcc
     3121 gaagattgcc gtgccgatgc gcaccatcgt tgcgccggca gcaatcgctt ccctgaagtc
     3181 ggccgacatg cccatcgaca atgtgtcgag ctcgatgccg gcgtgaacga gctgcgcgcg
     3241 cagagcagcg agctcggcga agcactgcgc ggccgcgttc tgcggcggta gcgtcatgag
     3301 gccgcgcagc ttcaggcgcg gcagctcgac gatcgcgcgt gcgagctcgg cgacatcggc
     3361 cggtgcggcg ccgctcttct gcgcttcgtc gccttgattg acttgtatca agacgttgag
     3421 gggcgctgca tgcgcaggac gctgctcgtt gagccgctcc gcgatccgca aacgatcgac
     3481 ggtgtgtacc cacgcgaagc gctctgcgac gtgccgagtc ttgttcgctt gcagcttgcc
     3541 gacgaaatgc caagttacgg ctaattcgcc aacggcgtcc atcttcggca gcgcttcttg
     3601 cacgtagctt tcgccgaagt gcgtgatgcc gacgcgatgc gcctcgacga tcgccgatga
     3661 cggttgctgc ttgctgatcg cgacgatcgt cacgctcgtt gcagagcgct gcgcgtgctg
     3721 cgctgcacga gcgacgcgct cgacgatatc tgccaaatgg tccgtgattt cagtcacgct
     3781 ttgccgggag cccttggcta tactgtcaac ttcttctgca gcccgttcgc agggctcggg
     3841 ctcgtgctag aggagtgcta tggccgcggt cgatattgcg caactgctcg catttgccgt
     3901 caagaacaac gcttcggact tgcacttgtc atcgggtgtc ccgccgatca ttcgcgtcga
     3961 cggagacgtc aagcgaatca acatgccgcc actgaagcac aaagaagtgc acagtatggt
     4021 ttacgacatc atgaacgaca agcagcgcaa ggacttcgaa gagttcttgg agactgactt
     4081 ctcgttcgag attccgaagc tcgcgcgctt tcgcgtgaac gcattcaatc aaaaccgcgg
     4141 ctgtgcggcg gtgttcagaa cgattccgtc ggtgatcttg agtctcgacg atctcggcgc
     4201 gccacgcatc ttcaaagaca tctcgatgca tccacgcggc ttggtgctcg tcacgggccc
     4261 gacgggctcg ggtaaatcga cgacgctcgc cgcgatggtc gactacgtca acgacaacaa
     4321 gcccgatcac atcatcacga tcgaagaccc gatcgagttc gtgcacgaga gcaagaagtc
     4381 gttgatcaac cagcgcgagt tgcatcgcga cacgcacggc ttcagcgaag cgctgcgctc
     4441 ggcgttgcgc gaggaccccg acgtcattct cgtcggcgaa atgcgcgacc tagaaacgat
     4501 tcgtttagcg ctgactgctg ccgagacggg ccacttggtc ttcggtactc tgcatacgag
     4561 ctctgccgcc aagacgatcg accgcgtcgt cgacgtgttc cctgccgccg agaaagagat
     4621 ggttcgcagc atgttgtcgg agtcgctgcg cgcggtcatt tcgcagacgc tgttgaaacg
     4681 cgtcggcggc ggccgtatcg ccgctcacga gatcatgatc ggcacgccgg ccattcgtaa
     4741 cctgattcgc gaaggcaaga tcgcgcagat gtactcggcc attcagaccg gccaggccca
     4801 gggcatgcag acgctcgatc agaatctgca ggagctgatg atgaagggcc tcatcacgaa
     4861 agaagaagcc cgcttcaagg ccgtcaacaa agagagtttc taggccagct ctcctgctct
     4921 ctacgctggc acttcagagg agcgcgtcgg aatggagcgc gatcaagcag tacgactgac
     4981 gcaaaatctg ttgaagaaga tggtcgagaa taacggctcc gaccttttca tcacggcgga
     5041 ttttcctccg gccatcaaga tcgacggcac gattcgaccg ttcgcggatc gcccgctgac
     5101 gtcggcgcag agctcgctga tcgtccgctc gatcatgaac gacaagcagg cgaaagaatt
     5161 cgacgcgacg aaggagtgca acttcgcgat cgcgccgcaa ggcatcggcc ggttccgcgt
     5221 cagcgcgttc gtccagcagg ggcacgtcgg cgcggtcatc cgcacgatcg tcacgaagat
     5281 tccgtcgttc gaggacctcg acctacccaa ccagctcaaa gacgtcgcga tgacgaagcg
     5341 cggcttgtgc ctggtcgtcg gcggaacggg gtccggtaag tcgacgacgc ttgctgcgat
     5401 gatcggtcat cgcaacgaga acagccgcgg ccacatcatc acgatcgagg atcccgtcga
     5461 gtacgtgcac ccgcaccgcg gctgcgtcgt aacgcaccgc gaggtcggcg tcgataccga
     5521 caattggtac aacgcgctga aaaacactct gcgtcaggct cccgacgtca tcatgatcgg
     5581 cgagatccgc gattccgaca cgatgaatta cgcgatccag ttcgcggaga ccggccactt
     5641 gtgcgtcgcg acgctacatg cgaacagtgc gaaccaggcg ctcgatcgca tcatcaactt
     5701 ctttcccgag gagcggcgcc accagctgct catggacctg tcgctgaaca tcacggcgat
     5761 gatctcgcag cgcctcctgc cgcgtgagaa caagatgggg cgtatcgcgg ccatggaaat
     5821 catgctcgcc tcgccgctga tcagtgacct catcttccgc ggcgaggtcg cgatgatcaa
     5881 agacatcatg gcgaagtcca accgcctcgg catgcagacg ttcgatcagg cgttgttcca
     5941 gctctacgag gacggcgcca tttcctacga agaggccatg cgcaacgccg actcgaagaa
     6001 cgagctccgg ctcaagatca agctgcagag caagcgcgag ggcgcctccg cggcggagca
     6061 cggcgagagc ttgcagttgg cggatgagga gaaggccggt cggttctaag cgtccgtcgc
     6121 ttcagctggc tctcgcatga acgtcaaacc gcttttcaag ctgatggtcg acaagaaggc
     6181 gtcggacctc ttctttacga cctatgcgcc cgtcatgatc aagatcgacg gcaagatcat
     6241 gcccgtcaac caggtcgagc tcacgccgaa gatggtgcgg caggcggcgc tcgagctcat
     6301 gaacgaacag cagctcgagc aattcacgag ggacctcgag ctcgacttcg cgatctcgga
     6361 gccgggcttg ggccggttcc gcgtcaacgt gttccaccag cgcggcaacg tcgcgatggt
     6421 gctgcgattc gtgacggccg agctaccgca gctcgacgcg ctcggaatgc cggcgatctt
     6481 gaaagacatg atcatgctgc ggcgcggcct gatcctgatg gtcggcgcga gcggctccgg
     6541 taagtcgacg acgctggccg cgatgatcga ctaccgcaac aagagctcgg cgtcgcacat
     6601 cgtcacgatc gaagacccga tcgagttttt gcacagcaac caaaagtcga tcgtcaatca
     6661 acgcgaggtc gggctcgaca ccttgtctta tcaccgcgcg ttgcgcagtg cgatgcgcga
     6721 agcaccggac gtcattctca tcggcgagat ccgcgaccgc gagacgatgc aggcggcggt
     6781 cgacctcgcc ggcacgggcc atctggcgat cgcgacgcta cacgcgaaca actcgcccga
     6841 gacgctcgat cgcatcatca acatgtatcc cgacgatcag cacaagcagg tgttgatgga
     6901 cctgtcgcag tacatccgcg tcatcatctc gcagcggctc gtgcgctcga aggaagggcg
     6961 gcgcgtggca gccgtcgagg tcatgctgaa cacgccgcac atctccgagt tgatcctgaa
     7021 gggcgacatc agccacgtga aagaagcgtt caaggacacg aaagagcccg ggatgcagag
     7081 cttcgaccaa gcgctgttcg acctgtatcg caagggcgcg atcagcatgg aagaggcgct
     7141 gtcgaacgcc gactcgcgcg ccaacctcga agccaaaatc aacttcggtt gacgcggcgc
     7201 gacgtcgtgg cgctgcgtcg tggcgcgcgc cgt
//
LOCUS       DATLEE010000294         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8365004, whole genome shotgun sequence.
ACCESSION   DATLEE010000294 DATLEE010000000
VERSION     DATLEE010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8365004"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VM692_06195"
     CDS             complement(<1..1122)
                     /locus_tag="VM692_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HUQ51793.1"
                     /translation="MAAPADAGRGVRGRATQAAVAETAVPLERIAINRKASGTIAVVG
                     NPNAGKSSVFNRLTGLRQHTANYPGVTVERRVGRASFGGVGLDLIDLPGTYSLSPTST
                     DEQIAVDVLFGRVAGTPRPEAILAVIDSTRLYQGLYLLQQLVELGRPMIVALTMIDAA
                     DREGRKIDCAALQEALGGVPVYPVVATSGRGFPALTEALARILTLAPPREAAHWPELK
                     RAAESLVARWRTQGADLHRVEAERLLIDASATPPTAQAGADVTAARAQVFGTEPPLAA
                     EARRRYAWVRATLARVETPGSGVLTQAARLIEWINRPWPATALLFIVMFVMFQAVFAW
                     ATPLVDLIDAGFSGLGSLAAAALPDGMLESFVVDGAIAGV"
     gene            complement(1106..1327)
                     /locus_tag="VM692_06200"
     CDS             complement(1106..1327)
                     /locus_tag="VM692_06200"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HUQ51794.1"
                     /translation="MQPGDRARIESIETADPAVQRLMVLGLVEGAELTFLRRSLGGDP
                     LEVRIYGAAISLRNEQARRFHVVPNGRAG"
     gene            complement(1405..>2269)
                     /locus_tag="VM692_06205"
     CDS             complement(1405..>2269)
                     /locus_tag="VM692_06205"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HUQ51795.1"
                     /translation="RRAIRVADLFAPRVLETKLERLLDLHNFLLEKYYRAERVDPRRE
                     LDNLLRLGERVKPLVVDVTETLRGLHTRGQSVLFEGAQGAMLDIDLGTYPFVTSSNTT
                     VGGAAAGTGVGPGAIDYVLGIVKAYTTRVGAGPFPTELDDDVGRRLRAVGQEFGATTG
                     RPRRCGWLDAVLLKRAGFTNSLTGLCLTKLDVLDGLPEIKICVGYRLDGKVMDTLPLL
                     IDRFGECEPVYESVPGWSAPTAGATAYSALPNEAKAYLTRIEELAGVPIDIVSTGPSR
                     DAVIMRRHPFD"
BASE COUNT          394 a          800 c          746 g          329 t
ORIGIN      
        1 gacgcccgcg atcgcgccgt cgacgacgaa actctcgagc atgccgtcgg gcaaagccgc
       61 agccgcgagc gagcccaaac cggagaagcc cgcgtcgatg agatcgacga gcggcgtcgc
      121 ccaagcgaac acggcctgaa acatgacgaa catcacgatg aacagcagcg ccgttgccgg
      181 ccacggccgg ttgatccact cgatgagccg ggcggcctgc gtcaggacac ccgagcccgg
      241 cgtctcgaca cgcgccaatg tcgcgcgcac ccatgcgtag cggcgccgcg cctccgccgc
      301 cagcggcggc tccgtgccga acacttgcgc gcgcgcggcc gtcacgtcgg cgcccgcctg
      361 cgccgttggc ggcgtcgcgc tcgcatcgat cagcaaacgc tcggcctcga cgcgatgcag
      421 gtcggcgccc tgcgtccgcc aacgcgccac gagcgactcc gcggcccgct tgagctcggg
      481 ccaatgcgcg gcttcgcgcg gcggggccag cgtcaaaatg cgcgcgagcg cctcggtcag
      541 cgccggaaag ccgcggcccg acgtcgcgac gacggggtac acgggcacgc cgcccaacgc
      601 ttcttgcaac gccgcgcagt cgatcttgcg cccttcacgg tcagcggcgt cgatcatcgt
      661 caacgcgacg atcatcggcc tgccgagctc gacgagctgt tgcagcaagt agaggccctg
      721 gtacaaccgc gtcgaatcga tgacggccaa gatcgcctcg gggcgcggcg tgccggcaac
      781 gcggccgaac agcacgtcga cggcgatttg ctcgtcggtc gacgtcgggc tcagcgagta
      841 cgtcccaggc agatcgatga gatcgagccc cacgccgccg aacgacgctc gcccgacgcg
      901 ccgctcgaca gtgacgccgg gataattggc cgtgtgctgc cggaggcccg tgagccggtt
      961 gaacacggag cttttgccgg cgttcgggtt gccgacgacg gcgatggtcc ccgacgcctt
     1021 gcggttgatg gcgatccgtt cgagcggaac ggcggtttcg gcaacggctg cctgcgttgc
     1081 tctaccgcgg acacccctgc ccgcgtcagc cggcgcggcc attcggcacc acatgaaagc
     1141 gtctcgcctg ctcgttgcgc agcgagatcg cggcgccata gatccggacc tcgaggggat
     1201 cgcccccgag ggatcggcgc aagaaggtga gctcggcccc ctcgacgagg cccaagacca
     1261 tcagccgttg aacggccggg tcggcggtct cgatcgattc gattcgggcg cgatcaccgg
     1321 gctgcaaatc cgcgagtgtc agacttcgac tccacgctca gcgcgcaaat aagaatcgtt
     1381 gtcatttaac accagatcgc cagctcaatc aaacggatgc cgccgcatga tgacggcgtc
     1441 acggctgggc cccgtcgaaa cgatgtcgat cggcacgccg gcgagttcct cgattcgagt
     1501 caaataggcc ttggcctcgt tcggcagcgc cgaataagcc gtcgcgccgg ccgtcggcgc
     1561 gctccagccc gggactgatt cgtagacggg ctcgcattcg ccgaagcgat cgatcagcag
     1621 cggcagcgtg tccatgacct tgccgtccaa gcggtagccc acgcaaatct tgatctcggg
     1681 cagcccgtcc aacacatcga gcttcgtcag gcagagcccc gtgaggctgt tcgtgaaacc
     1741 ggcgcgcttc agcagcacgg cgtcgagcca accgcagcgc cgcggccggc ccgtcgtcgc
     1801 accgaactcc tgcccgacag cgcgcagacg gcggccgacg tcgtcgtcga gctccgtcgg
     1861 gaacggcccg gcgccgacgc gcgtcgtgta cgccttgacg ataccgagca cgtagtcgat
     1921 ggcaccgggg ccgacgccgg tcccggcagc cgcgccgcct acggtcgtat tcgacgacgt
     1981 cacgaacgga tacgtgccga gatcgatgtc gagcatcgcg ccctgcgcgc cttcgaacag
     2041 cacgctttga ccgcgcgtgt gcaagccgcg cagcgtttcc gtgacgtcga cgacgagcgg
     2101 cttcacgcgc tcgccgagcc gcaataggtt atcgagctcg cggcgcgggt cgacacgctc
     2161 ggcacggtag tacttctcga gtaagaaatt gtgcaggtcg agcagccgct cgagcttcgt
     2221 ctcgagcacg cgcggcgcga atagatcggc gacgcgaatc gcgcgccgt
//
LOCUS       DATLEE010000295         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_338578, whole genome shotgun sequence.
ACCESSION   DATLEE010000295 DATLEE010000000
VERSION     DATLEE010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338578"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VM692_06210"
     CDS             <1..970
                     /locus_tag="VM692_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51796.1"
                     /translation="TWPNTARYPITGGAFWTSFALDTERGVLYVPAGNPAPDFDVQAR
                     GGDNLYSNSLIALDAATGRIVGYNQLVKRDEHDWDVNAPPALGVTRAGRPIVASANKD
                     GLLSVLDRSALAAAPQPAADLGTRMPTIFQSPTTTRENADVPLSRTARVRFCPGIQGG
                     NEWNGPAFHAGLNTLYTGAVDWCASVQLADEPSVPSAGAIWFGSAVGEIQAPASEAKG
                     WITAFDADSGAVRWKFAAPAPVVAGVTPTAGGLLFTADLTGRLRAFDADNGAVLFELE
                     TGQSIGGGIVSYSAGGKQRIGVASGMKSGIWPGAATESRIQVFALP"
     gene            complement(1007..2008)
                     /locus_tag="VM692_06215"
     CDS             complement(1007..2008)
                     /locus_tag="VM692_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HUQ51797.1"
                     /translation="MDLFQVSTTLVLLVALALAFDFMNGFHDAANSIATVVSTGVLKP
                     WQAVLWAAFFNVVAWAVFHLAVAATVGKGIVDPAFVDSRVIFGALIGAISWNFITWYY
                     GIPSSSSHALIGGLIGPALTKAGSAPLLWPGILKTAAFIFVAPLLGMALGALLMVAVS
                     WICFRQSPRRVDRWFRRLQLFSSAAYSLGHGGNDAQKTMGIIWLLLIANGLTTEDHLP
                     NWVVFSCFLAIGLGTMFGGWRIVKTMGQRITKLKPVGGFAAETAGSITLFMATYFGIP
                     VSTTHTITGAIIGVGTARGSSAVRWGVAGGIVIAWVLTIPASAGIAALAWWIGGLFF"
     gene            complement(2030..>2458)
                     /locus_tag="VM692_06220"
     CDS             complement(2030..>2458)
                     /locus_tag="VM692_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HUQ51798.1"
                     /translation="TTFVTPFDRDDIHRLISRMDDVLDLIQDAGESLVLYDIRQVTPE
                     ATQLAELLLRCTERVQAAVKLMASMADAPAMLRICEEIDKLESDADKVMRGAVSRLFR
                     EETDVRQLIKLKAVYEALESATDKCQDVANVIESVVLENA"
BASE COUNT          461 a          812 c          791 g          394 t
ORIGIN      
        1 gacgtggccg aacactgcgc gctacccgat tacgggtggg gcgttctgga catcgttcgc
       61 tctcgacacc gaacgcggtg tcctgtacgt gccggccggc aaccccgcac ccgatttcga
      121 cgtgcaggct cgcggcggcg acaacttgta ctcgaactcg ctgatcgcgc tagacgccgc
      181 gaccggccgc atcgtgggct acaaccagct cgtcaagcgc gacgagcacg attgggacgt
      241 caatgcgccg ccggcgctcg gcgtcacacg cgcaggacgg cccatcgtcg cgtcggcgaa
      301 caaggacggg ctcttgtcgg tgctcgaccg cagcgcgctc gcggccgcgc cgcaacccgc
      361 cgcggatcta ggcacgcgca tgccgacgat ctttcagtcg cccacgacga cgcgagagaa
      421 cgcggacgtg ccgctctcgc gcacggcgcg cgtgcgcttc tgccccggca tccagggcgg
      481 caacgagtgg aacggccccg cgttccatgc cggcctcaac acgctgtata cgggcgccgt
      541 cgactggtgc gcgagcgtgc agctcgccga cgagccgtcc gtgccgtcgg ccggcgccat
      601 ctggttcggc tccgcggtcg gcgagatcca agcgccggcc agcgaggcca agggttggat
      661 cacggctttc gacgcggaca gcggcgccgt gcgttggaaa ttcgcggcgc cggcgcccgt
      721 ggtcgccggc gtcacgccga cggcaggggg ccttttgttc acggcggacc tcacgggacg
      781 cttacgcgcc ttcgacgccg acaatggcgc cgtgctgttc gagctcgaga cgggtcagtc
      841 gatcggcggc ggcatcgtca gctactccgc gggcggcaag cagcgaatcg gcgtggcgtc
      901 cggtatgaag tcgggtatct ggcccggcgc cgcgaccgag agccgcattc aggtgttcgc
      961 tttgccatga gcgtggcccg cccctagcgc gcccgcggcg ctcggcttag aagaacaagc
     1021 cgccgatcca ccacgcgagg gccgcgatgc cggcggacgc cggtatcgtc aggacccaag
     1081 cgatgacgat gccgccggcg actccccagc ggaccgcgga agagccgcgc gccgtcccga
     1141 cgccaatgat cgcacccgta atcgtgtgcg tcgtagaaac ggggattcca aagtaggtgg
     1201 ccatgaacag cgtgatcgag ccggcagtct cagcagcgaa cccgccgacg ggtttcagtt
     1261 tcgtgatgcg ctgtcccatg gttttcacga ttcgccaacc gccgaacatc gtgccgaggc
     1321 ctatcgcgag aaaacaactg aacacgaccc agttgggcaa gtggtcttcg gtcgtcagac
     1381 cgttcgcgat gagcaatagc cagatgatgc ccatggtttt ttgcgcgtcg ttaccgccat
     1441 ggcccaggct atacgcagcg gacgaaaaaa gttgcagtcg gcgaaaccac cggtcgacac
     1501 gtcgaggcga ctggcgaaag cagatccacg atacggcgac catgagcaac gctccgagtg
     1561 ccataccgag caatggcgcg acgaaaatga acgccgccgt cttgaggatt cccggccaca
     1621 gtagcggtgc gctgccggcc tttgttagcg cgggaccgat caggccaccg atcagcgcgt
     1681 gcgacgagct cgaaggaatg ccgtagtacc aagtgatgaa attccaactg atggcgccga
     1741 tcaatgcgcc gaagatcacg cggctgtcga caaaggcggg atcgacgatg ccttttccga
     1801 ccgtcgctgc cacggccaag tgaaagacgg cccaagcgac cacgttgaaa aaggccgccc
     1861 acagtacggc ttgccacggc tttagcacgc ccgtcgacac gaccgtggcg atggagttcg
     1921 ccgcgtcgtg aaacccgttc atgaagtcga acgccaacgc gagtgccacc aacagcacca
     1981 gcgtcgtcga gacttgaaaa agatccatgc gtaggccgcc cggccgccgt caggcgttct
     2041 ctagcacaac gctctcgatg acgttggcga cgtcctgaca cttgtcggtc gcggactcga
     2101 gcgcctcgta gacagccttg agcttgatga gctgccgcac gtcagtttcc tcgcgaaaca
     2161 accgcgacac ggcgccgcgc atgactttgt cggcgtcgga ctcgagcttg tcgatctctt
     2221 cgcaaattct cagcatggcc ggcgcgtcgg ccatcgaggc catgagcttc acggcagcct
     2281 gcactcgctc cgtgcaacgc agcagcagct ccgcgagctg cgtcgcttcg ggcgtgactt
     2341 ggcgaatgtc gtacagcacg agcgactcgc cggcgtcctg gatcagatcg agcacgtcgt
     2401 ccatgcgcga gatcaaacga tggatgtcgt cgcggtcgaa cggcgtgacg aaagtcgt
//
LOCUS       DATLEE010000296         2316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4807036, whole genome shotgun sequence.
ACCESSION   DATLEE010000296 DATLEE010000000
VERSION     DATLEE010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4807036"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VM692_06225"
     CDS             complement(<1..438)
                     /locus_tag="VM692_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HUQ51799.1"
                     /translation="MSKQKTAWVDTDEAIALLGVSRATLYAYVSRGRIRSEAVRGGNG
                     RSRYSRDDLERLVERSRERRNPAKAAEQALHWGLPILESAITLIADEHIYYRGRDASV
                     LARTGSLADVAALLWTGAADGASLAAVKVEAAARAPRPAATVPF"
     gene            494..1576
                     /locus_tag="VM692_06230"
     CDS             494..1576
                     /locus_tag="VM692_06230"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HUQ51800.1"
                     /translation="MSTQIGSRANPGLEGIVAAETSLSLVDGERGELVVAGHALEELV
                     HHEFEDVVATLWGAAGVTVGARAAAAALPPATLALLADAAKRRIDPMDALRMATATLT
                     AAGDAAAARLLVGSFPTIVASYARLLRGEQPVAPRADLGLAANLLFMYTGAEPHVELV
                     RGLNVYLTTVADHGLNASTFTARVITSTGSDFVSAVTGAVGALKGPLHGGAPGPALDM
                     VFEIGDAARAEPMLRAKLARGERLMGFGHRIYKVRDPRADVLAAAAARMFARAGDSHL
                     YELARSVEATALKVLEEHKPGRRLKTNVEFYTALLLHGLGLDTAFFTPTFAVGRVAGW
                     TAHCFEQRAVGRLIRPQSAYVGRMPH"
     gene            1597..>2316
                     /locus_tag="VM692_06235"
     CDS             1597..>2316
                     /locus_tag="VM692_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HUQ51801.1"
                     /translation="MLWIFTTVIAAAVQTGRNAMQSNLTAALGTLGATQVRFIYGWPF
                     AVLFLAAAALATGAAIPTPTPRFLAFTTGGAVAQIAGTALLLAAMHARSFAVATVLSK
                     TEAVQVALFGIVILGEHLTVASGVACAIATAGVVLAAVGRGAKWDAASIKPALFGIAS
                     GGFFALASIGFRGGIVALGDGAYYLRAATTLTWALSLQTLLLGAWLAAFARPALVGSF
                     KLWRQSLLAGLLGASASQLWFL"
BASE COUNT          306 a          813 c          801 g          396 t
ORIGIN      
        1 gaacggcacg gtcgcagcgg gccggggggc gcgcgccgcg gcctcgacct ttacggccgc
       61 cagcgaagcg ccgtcggcgg cgcccgtcca gagcagcgcg gccacgtcgg ccagcgagcc
      121 cgtgcgtgcc agcacactgg catcacggcc gcggtaataa atgtgctcgt ctgcgatcaa
      181 cgtgatcgca gactcgagga tcggcagccc ccagtgcagt gcttgctccg ctgccttcgc
      241 gggattgcgg cgttcgcgcg agcgttcgac gagccgctcc agatcgtcgc gcgagtagcg
      301 agagcggccg ttaccgccgc gcacggcttc ggagcggatt cgaccacggc tgacgtaggc
      361 gtagagcgtc gcgcggctca caccgagcag cgcgatcgct tcgtcggtat cgacccaagc
      421 agttttctgt ttgctcatgt tgattgatga atcactattg actcatatgt gtatacacca
      481 tactttggcc cgcatgagca cgcaaattgg atcgcgcgcg aaccccggcc tcgaaggcat
      541 cgttgccgcc gagacctccc tttcgctcgt cgacggcgag cgcggcgagc tcgtggttgc
      601 cgggcacgcg ctggaggagc tcgtgcatca cgagttcgag gatgtcgtcg cgacgctctg
      661 gggcgcggcc ggcgtcacgg tcggcgcacg cgcggcggcc gcggctctgc cgcccgcgac
      721 tttggcgctg ctggccgacg cggcgaagcg acgcatcgat ccgatggatg cgctgcgcat
      781 ggccacggcc acgttgacgg cggcgggcga cgcggccgcc gcgcggctcc tcgtcggctc
      841 ttttcccacg atcgtcgcga gctacgcgcg cctgttgcgc ggcgagcaac cggtcgcacc
      901 gcgtgcggat ctcggcttgg ccgcgaatct gctcttcatg tatacgggcg cggagccgca
      961 cgtcgagctc gtgcgcggct tgaacgtcta tctcacgacg gtcgccgatc acggcttgaa
     1021 cgcgtcgacg ttcacggccc gcgtgatcac gtcgacgggc tcggatttcg tctccgcagt
     1081 cacgggcgcc gtcggcgcgc tgaagggacc cttgcacggc ggcgcgcccg gcccggcgct
     1141 cgacatggtg ttcgagatcg gcgacgccgc gcgcgccgag ccgatgctgc gcgcgaagct
     1201 cgcgcgcggc gagcgcctca tgggcttcgg ccaccgcatt tacaaggtgc gcgacccccg
     1261 cgccgacgtg cttgcggcgg ccgccgcacg gatgttcgcg cgcgccggcg attcgcacct
     1321 ctacgagctg gcacgctcgg tcgaagccac ggcgttgaag gtgctcgagg agcacaagcc
     1381 gggccgtcgg ctcaagacca acgtcgagtt ctatacggct ctgttgctgc acggcttggg
     1441 gctcgatacc gcgttcttca cgccgacgtt cgccgtcggc cgcgtcgcgg gctggaccgc
     1501 gcactgcttc gagcagcgcg ccgtcggccg gctgatccgg ccgcaaagcg cttacgtcgg
     1561 ccgcatgccg cactaggcga gaacgcgccg actccgatgc tttggatctt cacgaccgtc
     1621 atcgcggccg ctgtgcagac gggccgcaac gcgatgcaga gcaatctgac ggcggcgctc
     1681 ggcacgctcg gcgctacgca agtgcgcttc atctacgggt ggccgtttgc ggtcctgttc
     1741 ctggcggcgg cggctttagc gaccggcgct gccatcccga cgccgacgcc gcgcttcctc
     1801 gccttcacca cgggcggcgc cgtcgcgcag atcgccggga cagcgctgct gctcgccgcc
     1861 atgcatgcgc gctcgttcgc ggttgccacc gtgctcagca agaccgaagc cgtgcaagtg
     1921 gccctgttcg gcatcgtgat cctcggcgag cacttgaccg tcgcgagcgg cgtcgcgtgt
     1981 gcgatcgcga cggccggcgt cgtgctcgcg gccgtcggcc gcggcgcgaa atgggacgcc
     2041 gctagcatca agcccgcgct gttcggcatc gcgtcgggcg gcttcttcgc gctcgcgtcg
     2101 atcggctttc gcggcggcat cgtcgcgctc ggtgacggcg cgtattacct gcgcgccgcg
     2161 acgacgctga cttgggcact gagcctgcag acgctgctgc tcggcgcgtg gctcgcggcc
     2221 ttcgcacggc ctgcgctcgt cggcagcttc aagctgtggc ggcaatcgct gctcgcggga
     2281 ttgctcggcg catccgcctc gcagctctgg ttcctc
//
LOCUS       DATLEE010000297         9087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7620491, whole genome shotgun sequence.
ACCESSION   DATLEE010000297 DATLEE010000000
VERSION     DATLEE010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7620491"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VM692_06240"
     CDS             <1..245
                     /locus_tag="VM692_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUQ51802.1"
                     /translation="DWRASPYFTLGAGVINTDPKATLVATTDRTDSLAQAGAGARVYL
                     TRRFVFRAEYKASVVFTSRNDNEEVREWKAGFSFFF"
     gene            215..874
                     /locus_tag="VM692_06245"
     CDS             215..874
                     /locus_tag="VM692_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HUQ51803.1"
                     /translation="MESRVLVLFLTLALVVATLTGCGRDRLVRADDDGQPPVIEPDVE
                     RREIEPAKIDTEDFEVGIFAGQISVEDFGVNTVAGGRFAYHVTEGFFVELAAGKAKTE
                     LTSFERLSGAAQLLTDSERDYSYYNVSLGYNIFPGEGFLGRGRALNTATYVIGGVGKT
                     SFAGDDRFTVNFGVGMRIMPLDWFAVHADLRDHVFDIDLLGQEKTSHNLEAHVGVTFF
                     F"
     gene            899..1393
                     /locus_tag="VM692_06250"
     CDS             899..1393
                     /locus_tag="VM692_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HUQ51804.1"
                     /translation="MLRAFSVFVMTTLVASLAWAAQTLSGPAPGFSLMSRDGQQVSLA
                     ALKGNVVMVNFWATWCVPCRQEMPHLQALYERYHDLGFELLAVNVEKDKAAEVKKWLD
                     ETPVTFPVLFDPNNQVTKLYKVQTMPSTVLVARDGTMRFIHHGYKPGLEDEYQTQVRA
                     LLRE"
     gene            1422..1640
                     /locus_tag="VM692_06255"
     CDS             1422..1640
                     /locus_tag="VM692_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007619697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HUQ51805.1"
                     /translation="MVRAAALVAAAYALAGCSIEPWVKPYERDNLADPIMSFDRDPVS
                     SSYTGHVQEAREGARGATGAAGGGCGCN"
     gene            1649..2782
                     /locus_tag="VM692_06260"
     CDS             1649..2782
                     /locus_tag="VM692_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HUQ51806.1"
                     /translation="MKRCLLFALVSFAALRVADAGVLPEDRADVLLHSYDGGGVTIQG
                     PSVLVRKQFAQQFSVSANHYVDKVSSASIDVVTTASPYTEERTQHSIGIDYLHDRWMM
                     NVGFTNSEENDYAADTFSFGISQDIFGDLTTVSLGYSLGNDTVGRRGDANFHDEVERQ
                     QYRLGVSQILTKNLLMGLSFETITDEGFLNNPYRQVRYRDTTTPQGYSYEREVYPRTR
                     SSDAGSIRLRYYLPYRAAVHLEYRQYADTWDIQADTFELGYTHPIDSGWTIEGKFRTY
                     SQTKADFYNDLFSRSQFQNFMARDKELSTFTSQTIRLGGSYDIVRAGWKFVERGTVNV
                     VLDHMLFDYEDFRDLRRSGLTPGTEPFYTFDANVVQVFVSFWF"
     gene            complement(2930..4129)
                     /locus_tag="VM692_06265"
     CDS             complement(2930..4129)
                     /locus_tag="VM692_06265"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HUQ51807.1"
                     /translation="MIEVAEAEALIAAQMPRFRARHEPLAACGGRVLAEDVLAERDQP
                     PFDRVTMDGVAISFRDWARGVRSFEVQGTQAAGAAPLAVAAPAQCVEVMTGTPLPSGV
                     DTVVPVERVQRSGTIAAIEPTASVTAEQFVHRRGSDRLAGSVVLRAGTRLGPPEMAVL
                     AGAGRGNALVTDLPRVAVISTGDELVEAGVAVAPHQIRSTNDRAVEASVTQHRLGQVT
                     RARLRDDASALAVAIDRLDTELDVLVLSGGVSMGQFDFVPSVLTELGAKLVFHKVAQR
                     PGRPMWFGVSARGKPIFALPGNPVSTLVCATRYLLPALRQASGLTAAAPELVELTAPI
                     EASPTLTLFTPVTLSSSPRATLLAAPHPTNTSGDFVTLAGTDGFVELAAKNGTHPAGT
                     VARLFRW"
     gene            4222..4734
                     /locus_tag="VM692_06270"
     CDS             4222..4734
                     /locus_tag="VM692_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ51808.1"
                     /translation="MARILLCGLASCLTLSAAFGQPSNPELTQEQADSRTVIGVANEY
                     LAAGAEAIRAGQYDDGIRLTKLGLDRPASPRDRAAALSNLCAAHAAKNEPDTAIGYCT
                     ESLVINEANWRAYSNRAYAYYLKRQFEQADADLDIALSINPNAKQMPQIRGMMNERRL
                     RGRVTMEEHQ"
     gene            4779..5477
                     /locus_tag="VM692_06275"
     CDS             4779..5477
                     /locus_tag="VM692_06275"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HUQ51809.1"
                     /translation="MTIVSDYASAARAALDPVGTWSCVVWGHPEFGDERVLLNFDANG
                     IARLARVENDAVPAWSGLTPWVREDRQMRFSDPRTGRQFTADLRRENLGGNWRTLTTT
                     GGWWCAVSDVVVVPETTEERAAALPPVLPRVTATPRYPIAAIREAKQGRVVTCFLVDA
                     EGVIVEPEIIELSDEVFREPILVALSRSRYEARQADSALRPSCRSYTFSLDRLAQYEI
                     ESESESDVRELEFK"
     gene            complement(5479..7032)
                     /locus_tag="VM692_06280"
     CDS             complement(5479..7032)
                     /locus_tag="VM692_06280"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HUQ51810.1"
                     /translation="MNESQRNVFATALAFTLLSGCVINPVTGDRELALVSADQEIAIG
                     EQQYAPSQQMQGGEYTLDAELTSYVAGVGQKLAAVSDRQLPYEFVVLNSSVPNAWALP
                     GGKIAVNRGLLTELNSEAELAAVLGHEIVHAAARHGALAMQRGLLLQGALLATQVAAQ
                     RSDYGGLTVGAANLGAQLLTMRNSREAELESDLYGMRYMKEAGYDPSAAVTLQETFVR
                     LSEQNGGEGGRLAALFASHPPSTERVEKNRETLAALTAGGDLGRERYQAATARLRQRQ
                     PGYEAYDEGRKALADNRPADAERSAQQALRLLGAEGHVHALLGDIDLEQRRYQDAVRH
                     YADAIDRNDDFFYYHLQKGMAHRQLRQWEESRAELEASVQLLPTADAYYVLGTLAEQR
                     GDRQLALEHYERAAGGNSPAGKAAQDATVRLDLPANPGKYIQARGGLDNSGQLIVEVA
                     NPTGVPVTDIVVAVRYADGQGAIRELGRRINRLPAGQATRIATGLGPFTSSQQYEVGI
                     ADARVARAD"
     gene            7165..>9087
                     /locus_tag="VM692_06285"
     CDS             7165..>9087
                     /locus_tag="VM692_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ51811.1"
                     /translation="MALQPARRCAVALALLLLLEACHSSSNDDPPAPPGPNPPTVGLD
                     ARPSNLTCVAPAKTPAGVATIQLQRVFPNLSFSQPVGMLQAPGDNSRWFVLEKGGTVR
                     AVTTTANPTANAFITLAVNSGGEGGLLGMAFDPSFATNGFVFLSFTEGSPMVSVVARF
                     TIAPARTTLVTSSRLDILRVNQPSFENHKGGNIAFGPDGFLYLGLGDGGSGGDPSGHA
                     QNTRDLLGDFVRLDVRNATPATPYAIPAGAAGNPFQNNPICPATGGVQSCPEIYASGL
                     RNPWRWSFDTATGDLWVGDVGQNALEEIDLVERGGNYGWNCREGLVAYSGMPASCPAT
                     AAGFADPVHDYGRTAGASVTGGYVYRGTALPALVGRYVFGDFVTGRIWRLVPNGSGFA
                     AEELLDTALAISSFGQGNDGELYVVHIGSGASDGQLYKIVGGGSSGAGTPATPVPTLL
                     SATGCVNATTTAQPASGLIPYEPAAAFWSDGATKERWLGLPNSTSISVGSDGDFSFPN
                     GTVLMKHFRLNGNLVETRLFMRHPDGDWAGYSYEWNAQGSDATLVQGGKTVTIGSQSW
                     RFPSGNECLTCHTAAAGFALGVEAAQLNHDLTYGATRRTANQLRTLDGITLFATPLGD
                     PALQPSMPDPADTSAPL"
BASE COUNT         1545 a         2934 c         2986 g         1622 t
ORIGIN      
        1 ccgattggcg agcctcgccg tatttcaccc ttggtgccgg cgtcatcaac acggacccga
       61 aggccacgct cgtcgccacg accgacagga ccgattcgct cgcgcaagca ggagccggcg
      121 cccgcgtgta tctgacccgc cgcttcgtgt tccgcgccga gtacaaagcc agcgtggtgt
      181 tcacgagccg caacgacaac gaggaagtaa gggaatggaa agcagggttc tcgttctttt
      241 tctgaccctg gcgctcgtcg tcgcgacgct cacgggttgc ggccgcgatc gactggtgcg
      301 tgcggacgac gacggtcagc cgcccgtcat cgagcctgac gtcgagcggc gcgaaatcga
      361 gccggcgaaa atcgacaccg aggacttcga ggtcggaatt ttcgcgggcc agatcagcgt
      421 cgaggacttc ggcgtcaaca cggtcgccgg cggccgattc gcgtatcacg tgacggaagg
      481 cttcttcgtc gagctcgcag ccggcaaggc caagaccgag ctgacgagct tcgagcgact
      541 cagcggcgct gcgcagttgc tcacagactc cgagcgcgac tattcctact ataacgtgtc
      601 gctgggctac aacatttttc ccggcgaagg ttttctcggc cgagggcgcg cgctgaacac
      661 ggcgacttat gtcatcggcg gagtagggaa gacttcgttc gccggcgacg atcgcttcac
      721 ggtgaacttc ggcgtcggga tgagaatcat gccgctcgat tggttcgccg ttcatgccga
      781 cttgcgcgat cacgttttcg acatcgactt gctcgggcag gagaagacgt cccacaacct
      841 cgaggcgcat gtaggcgtca cgttcttctt ctgaatcttg cagctgcgga ggtagaccat
      901 gttgcgcgcg ttctcggttt tcgtgatgac gaccctcgtt gcgtccctcg cttgggccgc
      961 gcaaacgttg tcgggcccgg cgcccggttt ctcgttgatg tcgcgcgacg gccagcaagt
     1021 gtcgctcgcg gcgctgaagg gcaacgtcgt gatggtcaac ttctgggcga cgtggtgcgt
     1081 gccgtgccgc caggagatgc cgcacctgca agcgctctac gagcgctatc acgacttggg
     1141 attcgaattg ctcgcagtca acgtcgagaa ggacaaggcc gccgaggtca aaaagtggct
     1201 cgacgaaacg ccggtgacgt tccccgtgtt gttcgacccg aacaaccagg tgacgaagct
     1261 ctacaaagtt cagacgatgc cgagcacggt gctcgtggct cgcgacggca cgatgcgttt
     1321 catccatcac ggctataagc ccggcttgga ggacgagtat cagacccagg tgcgcgcgct
     1381 gctgcgggag taacgacatg actgtcgcga agctgtcgtc gatggttcgc gctgccgcgc
     1441 tcgtcgcggc cgcgtatgcg ctggccggct gcagcatcga gccgtgggtg aagccgtacg
     1501 agcgcgacaa cctcgccgac ccgatcatgt cgttcgatcg cgatcccgtc tcgagctcgt
     1561 acacggggca cgtacaggaa gcgcgcgaag gcgcccgcgg cgcaacgggc gccgccggcg
     1621 gcggctgcgg ctgcaactag gacactcagt gaagcgctgt cttcttttcg ctctggtttc
     1681 gttcgccgcg ctgcgcgttg ctgacgccgg cgtgctgccc gaggatcggg ccgacgtgct
     1741 gctgcattct tacgacggcg gcggcgtcac gattcagggc ccgtccgtcc tcgtccgcaa
     1801 gcagttcgcg cagcagtttt cggtctccgc gaaccactac gtggacaagg tcagcagcgc
     1861 ctcgatcgac gtcgtgacga cggcgagccc gtacaccgag gagcgcacgc agcactcgat
     1921 cgggatcgac tatctgcacg atcgctggat gatgaacgtc ggtttcacga acagcgaaga
     1981 gaacgattac gcggcggaca cgttcagctt cggcataagc caagacatct tcggcgatct
     2041 cacgacggtg tcgctcgggt actcgctcgg taacgacacg gtcggccgcc gcggcgacgc
     2101 caatttccac gacgaggtcg agcgccagca gtacaggctc ggcgtgtcgc agatcctgac
     2161 gaagaatctg ttgatgggcc tgtcgttcga gacgatcacg gacgaaggtt tcctcaacaa
     2221 cccgtatcgc caggtacgtt atcgcgatac gacgacgccg cagggctaca gctacgagcg
     2281 cgaggtctat ccgcgcacgc gctcgagcga tgccggctcg attcggctgc gctactactt
     2341 gccgtatcgt gcggccgtcc acctcgagta tcggcagtac gccgacactt gggacatcca
     2401 agccgatacg ttcgagctcg gttacacgca cccgatcgac tcgggttgga cgatcgaggg
     2461 caagttccgt acgtactcgc agacgaaagc ggacttctac aacgacttgt tctcgcgctc
     2521 gcagttccag aacttcatgg cgcgcgacaa ggagctcagc acgttcacga gccagacgat
     2581 ccggctcggc gggagctacg acatcgtgcg ggccggttgg aagttcgtcg agcgcggcac
     2641 ggtcaacgtc gtgctcgatc acatgttgtt cgactacgaa gacttccgcg atctgcggcg
     2701 ctcggggctg acgcccggca ctgagccgtt ctacaccttc gacgcgaacg tcgtgcaggt
     2761 gttcgtctct ttctggttct aaccggttgc accgcttgcg ccgttagcgc gaggcgaggc
     2821 catcccgaga cacgccggaa gaccgtccct ggggctcgtc ggcgcacatc cctgtgcgcc
     2881 gacggtctcg ggacgacccc gcctcacgct aacggcgcag tgccaccagt caccagcgga
     2941 agaggcgggc gacggtgccg gctgggtgag tgccattttt ggcggcgagc tcgacgaagc
     3001 cgtcggtgcc ggcgagcgtg acgaagtcgc ccgacgtgtt cgtggggtga ggggccgcca
     3061 gcagcgtggc gcgcggcgag gacgagagcg tcaccggcgt gaacagcgtt agcgtcggcg
     3121 atgcttcgat cggtgccgtc agctcgacga gctcgggtgc ggcggccgtg aggccggatg
     3181 cttggcgcag cgccggcagc agataacgcg tagcgcagac gagcgtcgag acgggattac
     3241 cgggcagagc gaaaatcggc ttgcctcgcg cgctgacgcc gaaccacatc ggccggccgg
     3301 ggcgctgcgc caccttgtga aagacgagct tcgcgccgag ctcggtcagc acggacggca
     3361 cgaagtcgaa ctggcccatc gagacgccgc cggagagcac gagcacgtcg agctccgtgt
     3421 cgaggcgatc gatggccacg gcgagcgcgc tggcgtcgtc gcgtagccga gcacgcgtga
     3481 cttggcccaa gcggtgctgc gtcacgctgg cctcgacggc gcgatcgttc gtcgagcgga
     3541 tctgatgcgg cgctacggcc acgcccgcct cgacgagctc gtcgcccgtc gatatcacgg
     3601 cgacccgtgg caaatccgtc accaaggcgt tgccgcggcc agcccccgcg agcacggcca
     3661 tctcgggcgg gccgagccga gtcccggcgc gcaacacgac gctgcccgcg aggcggtcgc
     3721 tgccgcgccg atggacgaat tgctcggccg tgacgctcgc cgtgggctcg atcgccgcga
     3781 tcgtgccgct gcgctgcacg cgttcgaccg gcacgacggt atcgacgccg ctcggcaacg
     3841 gagtgccggt catgacctcg acgcactgcg cgggcgcggc gacggccaac ggcgctgcgc
     3901 cagccgcttg ggtgccctgg acctcgaacg agcgtacgcc gcgagcccag tcgcggaacg
     3961 aaatcgcgac gccgtccatc gtcacgcggt cgaacggggg ttggtcgcgc tcggccagta
     4021 cgtcctcggc taggacgcgg ccgccgcaag cggccaaggg ctcgtggcga gcccgaaacc
     4081 gcggcatctg tgcggcgatc agggcctcgg cctcggcgac ttcgatcatg gtgcggcgat
     4141 catacgtaat acgcttcggc cggttgtggc gctgttccgg ccgacttggc ccggactata
     4201 ctaatccggg aggaggcaag catggcccgc attctgctct gcggcttagc aagctgtctc
     4261 acgctgagcg ccgcgttcgg tcagccgagc aatccggagc tcactcaaga acaagccgac
     4321 agccgcaccg tgatcggcgt cgcgaacgag tatctcgccg cgggcgccga agccatacgc
     4381 gcggggcaat atgacgacgg aattcgcctg acgaagttgg ggctcgaccg acccgcttcg
     4441 ccgcgcgatc gcgcggctgc actgtcgaac ctgtgtgcgg cacacgcggc aaagaacgag
     4501 cccgatacgg ccatcggtta ctgcaccgag tcattggtca tcaacgaggc gaattggcgc
     4561 gcctacagca atcgcgcgta cgcgtactat ttgaagcgtc aattcgagca agccgatgcc
     4621 gatctcgata tcgcgctgtc gatcaatccg aacgcaaaac agatgccgca gattcgcggg
     4681 atgatgaatg agaggcgcct gcgcggccgc gtcacgatgg aggaacacca ataattcgct
     4741 ggacacacac ggtagctgtt gcggccgtct ccgcgtgcat gacgatcgtg agcgactacg
     4801 cgagcgccgc gcgcgccgcg ctcgatccgg tcggcacttg gagttgcgtc gtttggggtc
     4861 accccgagtt cggcgatgag cgcgtgctgt tgaattttga cgcgaacggc atagcgcgtt
     4921 tggctcgcgt cgagaacgat gccgtgccgg cgtggagcgg tctgactccg tgggtccgcg
     4981 aggaccggca aatgcgcttc agcgatccac gcaccggccg ccaattcacg gcggatctgc
     5041 gccgcgagaa ccttggcggc aattggcgca cgctgacgac cactggcggc tggtggtgtg
     5101 ccgtgtcgga cgtggtcgtc gttcccgaga cgaccgagga acgcgccgct gcgttgccgc
     5161 ccgttctgcc gcgcgtgacg gccacgccgc gctacccgat cgcggccatt cgcgaagcga
     5221 agcagggccg tgtcgtgacc tgttttctcg tcgatgcgga aggagtcatc gtcgagcccg
     5281 agatcatcga gctgtcggac gaagtgttcc gcgagcccat actcgtcgcg ctgagtcgtt
     5341 cgcgctacga agcgcggcaa gcggatagtg cgttgcgacc gagttgccgg agctacacgt
     5401 tcagcctcga cagacttgct caatacgaga tcgagtcgga gtcggagtcc gacgtgcgcg
     5461 agctcgagtt caagtgagtt aatcggcgcg cgcgacgcgc gcgtccgcga ttcccacctc
     5521 gtactgttgc gacgacgtga acggcccaag tcccgttgcg atgcgcgtgg cttgcccggc
     5581 cggcaatcgg ttgatccgcc gcccgagctc gcggatggcg ccttgcccat ccgcgtagcg
     5641 caccgctacg acgatatccg tgaccggcac gcctgtcggg ttcgcgacct cgacgatgag
     5701 ctgccccgag ttgtcgagac cgccgcgcgc ttgaatgtac ttgcccgggt tggccggcag
     5761 atcgagccgg acggtcgcat cctgcgccgc tttaccggca gggctgttgc cgccggccgc
     5821 tcgctcgtag tgctcgaggg ccagctggcg gtcgccacgt tgctcggcca gcgtgccgag
     5881 gacgtagtac gcgtcggccg tcggtaagag ctgcacgctc gcctcgagct ccgcgcgcga
     5941 ctcctcccac tggcgcagtt gccggtgcgc catgcctttc tgcaagtggt agtagaagaa
     6001 gtcgtcgtta cgatcgatcg catcggcgta gtggcgcacg gcgtcctggt aacgccgttg
     6061 ctcgaggtcg atgtccccga gcagcgcgtg aacgtgccct tcggcgccca acaatcgcag
     6121 cgcctgttgc gccgagcgct cggcatcggc cggccgattg tcggcaagcg ctttacgacc
     6181 ttcgtcgtag gcctcgtagc cgggttgtcg ctggcgcagc cgagccgtag cggcttgata
     6241 gcgctcgcgg ccgagatcgc cgccggccgt caacgccgcg agcgtctcgc gattcttctc
     6301 gacgcgttcc gtcgaaggcg gatggctcgc gaacaacgcc gccaggcggc cgccctcgcc
     6361 gccgttctgc tcggacaagc gaacgaacgt ctcttgcagc gtgacggccg cgctcgggtc
     6421 gtagcctgct tccttcatgt agcgcatgcc gtagagatcc gattcgagct ccgcttcgcg
     6481 gctgttgcgc atcgtcagca gctgcgcgcc gaggttcgcg gcaccgacgg tcaggccgcc
     6541 gtaatcgctg cgttgcgctg cgacttgggt cgcgagcaat gcgccttgca acaacaagcc
     6601 gcgctgcatt gccagcgctc cgtggcgtgc cgccgcgtgc acgatctcgt ggccgagcac
     6661 ggccgcgagc tcggcttccg agttgagctc cgtcagcaag ccgcgattga cggcgatctt
     6721 gccgcccggc aacgcccagg cattcggcac cgagctgttc aacacgacga actcgtacgg
     6781 caactgacgg tcgctgacgg ccgcgagctt ttgccctacg cctgcgacgt acgacgtgag
     6841 ctcggcgtcg agcgtgtact cgccgccctg catttgttgc gacggggcgt attgctgctc
     6901 gccgatcgcg atctcttgat cggccgacac gagcgcgagc tcgcggtcgc ccgtcacggg
     6961 gttgatgacg cagccgctca agagcgtgaa cgcgagcgct gtggcaaaga cgttgcgttg
     7021 tgactcgttc atgtcgttca cccgtcgcaa ttcacgactc gtatattaag ttgcgtcgcc
     7081 gaaatccgac aaaagttctc ttgttttcag aaccgtaata cggccagcat ggactcacat
     7141 ttctcaaaac aaggaggtcc gcacgtggct ttgcagcccg ctcgccgatg cgccgtcgcg
     7201 ctggccctat tgttactgct agaagcgtgc cactcgtcgt cgaacgacga cccgcccgcg
     7261 ccgccgggac cgaatccacc gaccgtcggg ctcgacgcac ggccgtcgaa tttgacttgc
     7321 gtcgcaccgg caaagacgcc cgccggcgtg gcgacgattc agttgcaacg cgtcttcccc
     7381 aacctgtcgt tcagtcagcc ggtcgggatg ctccaagctc ccggcgataa ctctcgctgg
     7441 ttcgtgctcg agaagggcgg caccgtgcgc gcggtcacga ccacggcaaa tcccacggca
     7501 aacgcattca tcactctcgc cgtgaacagc ggcggcgaag gcggccttct cggcatggcc
     7561 ttcgatccga gtttcgccac gaacggattc gtgtttctgt ccttcaccga aggctcgccg
     7621 atggtgtcgg tggtcgcgcg gttcacgatt gcgccggcgc gtacaacgct cgtgacgtcc
     7681 agcaggctcg atatccttcg agtcaaccaa ccttcgttcg agaatcacaa aggcggcaac
     7741 atcgcgttcg gcccggacgg attcttgtac ctgggtttgg gtgacggcgg ttcaggaggc
     7801 gatcctagcg ggcatgcgca aaacactcgc gacttgttgg gcgatttcgt ccgcttggat
     7861 gtaagaaacg caacgccggc gacgccttac gcgatcccgg ctggtgcggc aggcaacccc
     7921 ttccagaaca accccatttg cccggcgact ggtggcgtgc agagctgccc cgagatctat
     7981 gcctcgggct tacgcaatcc ctggcgctgg agctttgaca ccgctaccgg cgacctctgg
     8041 gtcggtgacg tcggccaaaa tgcactcgag gagatcgacc tcgtagagcg cggcggcaac
     8101 tatgggtgga actgtcgcga agggttggtt gcttacagcg gcatgcccgc gagctgtccc
     8161 gcgacggcgg ccgggtttgc cgatccggtg cacgattacg ggcgaacagc gggagcttcg
     8221 gtcacgggcg gttatgtgta tcgcggaacc gctctgccgg ctctcgttgg gcggtatgtg
     8281 ttcggcgatt tcgtgacggg ccgtatctgg cgcttggttc cgaacggcag tggcttcgcc
     8341 gcggaggagc tgctggacac cgccttggcc atctcgtcgt tcggacaggg caacgacggt
     8401 gagctctacg tcgtccacat tggcagcggc gcttccgacg gccagctgta caagatcgtc
     8461 ggcggcggga gctccggcgc gggcacgcct gcgacgcccg tgccgacctt gctatcggcg
     8521 acgggctgcg tcaacgcgac gactaccgca caaccggcca gcggcttgat tccgtacgag
     8581 ccagcggcgg cgttctggtc cgacggcgcg acgaaggagc gttggctcgg gttgccgaac
     8641 agcacgtcga tcagcgtcgg cagcgacggc gacttcagtt tcccgaacgg caccgtgctc
     8701 atgaaacact tccgcttgaa cggtaatctg gtcgaaacgc gcttgttcat gcgccatccc
     8761 gacggcgact gggccggcta tagctacgag tggaatgcgc agggcagcga tgcgacgctc
     8821 gttcaaggcg gtaagaccgt gacgatcggc tcccagagct ggcgcttccc gagcggcaac
     8881 gagtgcctga cgtgccacac ggcggccgcc ggtttcgcgc tcggcgtgga ggcggcgcag
     8941 ctgaaccacg atctgaccta tggggcgacg cgccgcaccg caaatcagct gcgcacgctc
     9001 gacgggatca cgttgttcgc gacgccgctc ggcgatccgg cgctgcagcc ctcgatgccc
     9061 gatccagccg atacgtcggc gcctctc
//
LOCUS       DATLEE010000298         4819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9862368, whole genome shotgun sequence.
ACCESSION   DATLEE010000298 DATLEE010000000
VERSION     DATLEE010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9862368"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /gene="glyA"
                     /locus_tag="VM692_06290"
     CDS             complement(<1..541)
                     /gene="glyA"
                     /locus_tag="VM692_06290"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132485.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HUQ51812.1"
                     /translation="MSDMPALRATDPDIHRLIELETRRQHDYIRLIPSENYASAAVME
                     ATGSILNNKYSEGYPGKRYYEGQQYIDQVETLAIERAKSLFGMQHANVQPYSGSPANQ
                     AVYLALAKPGDPVMGLGLPFGGHLTHGWNVNFSGIVYHASQYDVDPETHRIDLNRVES
                     LAKSTRPKLIFCGGTAYPRV"
     gene            complement(628..2619)
                     /locus_tag="VM692_06295"
     CDS             complement(628..2619)
                     /locus_tag="VM692_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ51813.1"
                     /translation="MTSSFDARRASLPFAVALLLAAVRPSAAQSADSPEEVVVTGVPL
                     KHAPGELPQSITVVGGDELDRSRAATLGETLANQVGMSSSYFGAGASRPIIRGLAGAR
                     VRMLEDGVDSMDVATVSDDHAVTVDPLAADQIEIFRGPTTLLYGSGAVGGVVNTVTTR
                     IPMRAPEDGFEGAVELRGDTVADARGGALRLDAGRERFAWHFDAAHRDSGDFDIPGFA
                     RVAPQLGEPHGVLLNSAAESNSAAFGAAWLGDSGFLGASFNAYDTLYGVPSDEAAPVR
                     IDLEQRRLDVRGGWMELAGFVERVTLRLAGNDYEHVELEGDEPGTQFTNDGVEARIEL
                     EHRALGAWKGAFGAQLGGREFAAIGAEAFVPPVDSSSMGLFAVEQLDLTQWQVSLGGR
                     IELERQTPANGLPRVEHDAGSLSFGAVRPFGRGLSLALSLASSERLPVAEELYSDGPH
                     LATGVVQTGSAELGAEQAHHLDVGVRGERGELKWAVTTFATRYHDFIYLAATGDSDAD
                     TGLPAFAYTASAAEFAGLEAELFTPIATVKKGELDMRVFADVVRGELATNEPVPRLPP
                     LRFGGRLQYHNERVLVGLEAAHYGEQNRLAAFETPTAGYTLVNADVRWRVERSGKELE
                     WFVNASNLGDEEARKHTSFVKEIAPLPGRNYALGIRSRF"
     gene            complement(2691..3014)
                     /locus_tag="VM692_06300"
     CDS             complement(2691..3014)
                     /locus_tag="VM692_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51814.1"
                     /translation="MQRRTLMQRCRSASWKRWLGAALVVWLLLAESFAVTHPLDRAAH
                     ADGQPCAVCLSVANLGAGAVAAEIAVALEAAALLIVVAVVVALVSSAPVRRYARGPPA
                     VSFAF"
     gene            3094..4332
                     /locus_tag="VM692_06305"
     CDS             3094..4332
                     /locus_tag="VM692_06305"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HUQ51815.1"
                     /translation="MSKRPRFVLALALVASLRLQAELPPVPVPVENPITEPKRVLGKI
                     LFWDEQLSSDGSVACGTCHRPGAGGGDPRSGRHPGIDKGTIDDVLGSPGIVALDHDGK
                     PRPNALFGAGPQITPRLSPANFTALWADALFWDGRAGPELEDPLTGKVAIARGGALEN
                     QALAALLNDTEMAKAGRTWADVAADLARSRPLALAAELPPDTAAAIERHPTYPALFAA
                     AFGDAAITPLRIAFALATYQRTLVADQTPWDRFAAGDTTALSGRALYGWRALQSFHCT
                     ACHTPPLVTNNEFFQIGVRRADFDRGRQNVTHDPEDAGEMKVPSLRNAALRPRFMHTG
                     EFASLGAAIRFYINTLALPERDGIPGAGLYTFNMSGIDEADLREFITAALTDPRVRDE
                     RFPFDRPVLRSERAGDVAQP"
     gene            4389..4781
                     /locus_tag="VM692_06310"
     CDS             4389..4781
                     /locus_tag="VM692_06310"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HUQ51816.1"
                     /translation="MGKPAGNTGFRRLVNATFFSFAGLRAAWREEAAFRQEVLLCIVL
                     VPTAVWLGQTAVERALLIGSCLLVLVVELLNSGLEAVVDRIGTEHHRLSGQAKDLGSA
                     AVLMSLLLVLVIWALIVWQRFGASASGS"
BASE COUNT          753 a         1684 c         1639 g          743 t
ORIGIN      
        1 aaactcgcgg atacgccgtg ccaccgcaga agatcagctt cggacgcgtc gacttcgcga
       61 gcgactcgac gcggttcaag tcgatgcgat gtgtctccgg gtccacgtcg tactgcgacg
      121 cgtgatagac gatgcccgag aagttcacgt tccagccgtg cgtcagatgc ccgccgaacg
      181 gcaggccgag ccccatgacg ggatcgccgg gcttcgcgag cgcgagatac accgcctggt
      241 tggccggcga gcccgagtac ggctgcacgt tggcgtgctg catgccgaac aaactcttcg
      301 cacgctcgat cgccagcgtc tcgacctggt cgatgtactg ctgcccttcg taatagcgct
      361 tgccgggata accttccgag tacttgttgt tcaggatcga gcccgtcgcc tccatgacgg
      421 cggccgacgc gtagttctcg gacgggatca gccggatgta gtcgtgttgc cgccgcgtct
      481 cgagctcgat gaggcggtgg atgtcggggt cggtcgcgcg caaggcgggc atgtcggaca
      541 ttcggattcc ttggacgggg agctggcggc gctgctggca gcctaaggga gcaatagttt
      601 acaggacggc gcgcccgcgc agcacgacta gaaccggctg cgaatgccga gcgcgtaatt
      661 gcgccccggc agcggcgcga tctccttgac gaacgacgtg tgcttgcgcg cctcttcgtc
      721 gccgagattg ctcgcgttga cgaaccactc gagctccttg ccgctgcgct cgacgcgcca
      781 gcggacgtcg gcgttgacga gcgtgtagcc ggcggtcggc gtctcgaacg cagcgagtcg
      841 gttttgctcg ccgtaatgcg cggcctcgag tccgactaga acccgttcgt tgtgatattg
      901 caggcgcccg ccgaaacgca gcggcggcaa gcgtggcacc ggctcgttcg tcgcgagctc
      961 gccgcgcacg acgtcggcga atacgcgcat gtcgagctcg cctttcttga cggtcgcgat
     1021 cggcgtgaac agctcggctt cgaggcccgc gaactcggcg gcgctcgcgg tgtacgcgaa
     1081 cgccggcaac cccgtgtcgg cgtcgctgtc gccggtcgcc gcgaggtaga tgaagtcgtg
     1141 gtagcgcgtg gcgaatgtcg tgacggccca cttgagctcc cctctctcgc cgcgcacgcc
     1201 gacgtcgaga tggtgcgctt gctcggcgcc gagctccgca gagccggtct gcacgacgcc
     1261 ggtcgcgaga tgcgggccgt ccgagtagag ctcctcggcg accggcagac gctccgacga
     1321 ggcgagactc aacgccagcg agaggccgcg gccgaagggc cgcacggccc cgaacgatag
     1381 gctgcccgca tcgtgctcga ctcgcggcaa gccgttcgcc ggcgtctgcc gctcgagctc
     1441 gatacggccg ccgagcgaca cttgccattg cgtaagatcg agctgctcga ctgcgaacaa
     1501 gcccatgcta gacgagtcga cgggtggcac gaacgcttcg gcaccgatcg cggcgaactc
     1561 gcgcccgccg agctgcgcgc cgaacgcgcc cttccatgcg ccgagcgcgc ggtgctcgag
     1621 ctcgatgcgc gcctcgacgc cgtcgttcgt aaactgcgtg cccggctcgt cgccttcgag
     1681 ctcgacgtgc tcgtaatcgt tgcccgcgag ccgcaacgtc acgcgttcga cgaaccccgc
     1741 gagctccatc caaccgccgc gcacgtcgag gcgccgctgc tcgaggtcga tgcgcacggg
     1801 cgccgcctcg tcgctcggca cgccgtagag cgtgtcgtag gcgttgaagc tcgcgccgag
     1861 aaagccggaa tcgccgagcc acgcagcgcc aaaggccgcc gagttgctct cggccgcgct
     1921 gttcagcagt acgccgtgcg gctcgccgag ctgcggcgcg acgcgcgcaa agcccggaat
     1981 gtcgaagtcg ccgctgtcgc ggtgcgcggc gtcgaagtgc cacgcgaagc gctcgcggcc
     2041 ggcatcgagc cgcaacgcgc cgccgcgggc atcggccaca gtgtcgccgc gcaactcgac
     2101 ggcgccttcg aagccgtcct cgggcgcgcg catcggaatg cgcgtcgtca cggtgttgac
     2161 gacgccgccg accgcgccgc tgccgtatag caacgtcgtg gggccgcgaa agatctcgat
     2221 ctggtccgct gcgagcggat cgaccgtgac ggcgtggtcg tcgctgacgg ttgcgacgtc
     2281 catggagtcg acgccgtctt cgagcatgcg cacgcgcgcg ccggccaagc cgcgaatgat
     2341 cggccgcgac gcacccgcgc cgaagtacga cgagctcata ccgacctggt tggccagcgt
     2401 ctcgccaagt gtcgccgcgc ggctgcgatc gagctcgtcg ccgccgacga ccgtgatcga
     2461 ttgcggcagc tcgcccggag cgtgcttgag cggcactccg gtgacgacga cttcctccgg
     2521 cgagtctgcg ctctgcgccg cggacggccg cacggccgcc aggagcaggg cgactgcgaa
     2581 cggcaaagac gcgcgacgcg cgtcgaatga gctggtcatg aaacgtttcc ttcgaagaga
     2641 gacggtctga tggagcgacg gccgcggctc gcggtgagcc ggcggctact tcagaacgcg
     2701 aaggaaacgg cgggtggacc tcgcgcgtac cggcggaccg gggcgctcga aacgagtgcg
     2761 acgacgacgg cgacgacgat cagcagcgcg gccgcctcga gcgcgacggc gatttccgcc
     2821 gcgacggcgc cggcgccaag gttggcgacg ctgaggcaaa cggcgcacgg ctggccgtcg
     2881 gcatgcgcgg cgcgatcgag cggatgcgtg acggcgaacg actcggccag cagcagccaa
     2941 acgacgagcg ccgcgccgag ccatctcttc caggaggcgc tgcggcatcg ctgcatcaag
     3001 gtccggcgct gcattggaaa actctagcct cggcggccgc agctgtcgag gccgcagacc
     3061 gcagctgccc ggcgactcct ttagaatgcg ccgatgtcga aacgaccgcg attcgtgctc
     3121 gcgctggcgc tcgtggccag cctgcggctc caggccgagc tgccgccggt gcccgtgccg
     3181 gtcgagaatc cgatcacgga gccgaagcgc gtgctcggca agatcttgtt ctgggacgag
     3241 cagctgtcga gcgacggcag cgtcgcctgc ggcacctgcc atcggcccgg cgcgggcggc
     3301 ggcgatccgc gcagcgggcg tcatcccggc atcgacaagg gcacgatcga cgacgtcctc
     3361 ggctcgcccg gcatcgtcgc gctcgaccac gacggcaagc cgcggccgaa cgcgttattc
     3421 ggcgcgggcc cgcaaatcac gccgcggctc tcgccggcca acttcacggc gctttgggcc
     3481 gacgcgctct tttgggacgg ccgcgccggc cccgagctcg aggacccgtt gaccggcaag
     3541 gtcgcgatcg cgcgcggcgg cgcgctcgaa aaccaagcgc ttgcggcgct gctcaacgac
     3601 accgagatgg ccaaggcggg ccgcacgtgg gccgacgtag cggccgatct cgctcgttcg
     3661 cggccgctcg cgctggccgc cgagctgccg cccgataccg cggccgccat cgagcgccac
     3721 ccgacctacc ctgcattgtt cgcggcggca ttcggcgacg ccgcgatcac gccgctgcga
     3781 atcgcgttcg cgctcgcgac atatcaacgc acgctggtcg ccgaccaaac gccgtgggac
     3841 cgattcgccg ccggcgacac gactgcgctg agcggccgcg cactttacgg ttggcgcgcc
     3901 ctgcaatcgt tccactgcac ggcgtgccac acgccaccgc tagtcacgaa caacgagttc
     3961 tttcagatcg gcgtgcgccg cgccgacttc gatcgcggcc gccagaacgt cacgcacgat
     4021 cccgaggacg ccggcgaaat gaaggtgccg agcttgcgca acgccgcgct gcggccgcgg
     4081 ttcatgcaca cgggcgagtt tgcgagcctc ggcgccgcca ttcggttcta catcaacacg
     4141 ctggcgctgc ccgagcgcga cggtatccct ggcgccggcc tctacacctt caacatgagc
     4201 ggcatcgacg aggccgacct gcgcgagttc atcacggcgg cgctgacgga tccgcgcgtc
     4261 cgcgacgaac gtttcccgtt cgatcggccc gtgctgcgca gcgaacgcgc gggtgatgtc
     4321 gcgcaaccct gaacgcgacg ccatgaccct gctaccatcc gcgcactttc ccgaccgaaa
     4381 gcgcgctcat gggtaagccc gccggcaata ccggcttccg ccgcctcgtc aacgccacgt
     4441 tcttctcatt cgcgggcctg cgcgccgcct ggcgcgagga agctgccttt cgccaggagg
     4501 tattgctctg catcgtgctc gtgccgaccg ccgtctggct cgggcaaacg gccgtcgagc
     4561 gcgcgctgtt gatcgggtcg tgcctgctcg tgctcgtcgt cgagctgttg aactccggcc
     4621 tcgaagccgt cgtcgaccgc atcggcaccg agcatcaccg gttgtcgggg caagcgaaag
     4681 atcttggctc cgctgccgtg ctgatgagtc tgctactcgt gctcgtcatc tgggccctga
     4741 tcgtctggca gcgattcggt gcgagcgcga gcgggtcgtg aacgacgacc tcgaccggcg
     4801 ccacggttgg tcgaagcag
//
LOCUS       DATLEE010000299         7056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9282202, whole genome shotgun sequence.
ACCESSION   DATLEE010000299 DATLEE010000000
VERSION     DATLEE010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9282202"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VM692_06315"
     CDS             <1..777
                     /locus_tag="VM692_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HUQ51817.1"
                     /translation="ADLALGETKVPVKMYSAVQDAKVHFRLLHAADHAPVKQQMVDPA
                     TEQPVVATQTKKAVAVDRGVYVLLTEEEQVALAPKPSRLISIEQVVPADAIDERWFDR
                     PYYLGPDGDEEGYFALAEALDKRGAVGVAHWVLRNRSYVGALQASGGYLLLDTLRYAE
                     QIVEVGAVQPSANRAPDKREIALAEQLIGALEDRFDATEFRDEYQEQVRNMIAAKAAG
                     KVVRFPKAPKREPTDSLVASLQASLKASRKDVGAGHGRRA"
     gene            761..1798
                     /locus_tag="VM692_06320"
     CDS             761..1798
                     /locus_tag="VM692_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HUQ51818.1"
                     /translation="MAEEPEAPEAQPNPRAFWSGTLTFGLVSVPVDLYPAVRPRRTPL
                     RMLGPEEQPLVRRYFCSIDGEALTNDDIVRGYENDDGSFTIVEDEELAALAPRKSRDI
                     DLRRFVARDEIPRQLLERPYVLAPAGDSTKAYHLLAATMESSDRAGIATFVMRGKEYL
                     AAIFAEGGTLRAVTMRFVAELRTPQDVGLPRPARAAEERRREMERALAELTADELDTK
                     LLEDDYATKLLELAALKREAGQDVVEIEEAPADDEEEGADIIDIMTVLRERMGAARGP
                     ARDPGKSAKAGAEPPAREHGDDALAGKSKKELYEQAQKLKLPGRSAMSRDELIAALRP
                     PSGTSKGRKAG"
     gene            complement(1799..4363)
                     /gene="ligD"
                     /locus_tag="VM692_06325"
     CDS             complement(1799..4363)
                     /gene="ligD"
                     /locus_tag="VM692_06325"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HUQ51819.1"
                     /translation="MKKAAGGASRKLAPYRAKRHFNKTPEPSGSQRAAPAGQGALQFV
                     VQKHDATRLHYDFRLEHDGVLKSWAVPKEPSLSTKDKRLAVQTEDHPLDYGGFEGEIP
                     EGEYGAGSVVIWDRGHWQPLNDADEGLKEGKLDFELDGERLKGRFTLVRMADRPSSGT
                     SKGRGKRKSDKHNWLLLKRTDRPEAERKTATPRPSSGTPKGRLPSGTSKGRPPSGTSK
                     VRVRRKQSAPDPEGVPAARRAALPKTPSPQLTTPVTRPPDGPGWLFEPKLDGYRVLCR
                     IDDGDVTVLTRRGNDWTSEFTAIAAAAKKLPCRTAILDGEAVVFDSHGVTSFQRLQNA
                     LKGDSSQIELVAFDLVHLDGFDLTRVPLLERKRLLEQLLEGAPAAIRFGEHVTENGAK
                     FFAAACKLGLEGIIAKREADPYRQERTRSWLKIKCLKREEFVVVGFTDPGGSRTAFGA
                     LLVATRSDAAAPLRYAGKVGTGFDERTLRALHARLRPLERRLPPVERASARGVARGVH
                     WVEPELVAEIAYTEWTSDGRLRHPIYVGLREDKTAAEVVEERAEAPVERTESAPAATP
                     AAGPKAQVRLTHPDKILFPDPGITKRQLADYWREVADVALPLLRGRPLTLRRCPEGYA
                     GQCFWQKHVGVGVPAAVPRVAITEGEDPYAAIDDVASLQALVQIGVLEMHVWGSRAEH
                     LDQPDIIVLDLDPAEDVPWHEVVRAGFDVKGRVEALGLNAFARVTGGKGLHVVVPVTP
                     GPTWPAVKKFTHAFVNEMVRAEPRRFIASMSKTKRVGKIFIDYLRNDAEATAIGSYSP
                     RARAGAPVALPIEWDELDAKAAQAPRYGLLEVPALVRRRKRDPWEGFEAARRALVD"
     gene            complement(4311..5237)
                     /locus_tag="VM692_06330"
     CDS             complement(4311..5237)
                     /locus_tag="VM692_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUQ51820.1"
                     /translation="MSTRALIGISGWRYAPWRGDFYPAGLPQREELAYAAAHFPTIEL
                     NGSFYSLQRPEYYECWYDQVPASFVFAVKGSRYITHMLRLKGVATALANFFASGVLKL
                     RHKLGPLLWQLPPTFKFDEARLAEFFALLPRSTKEAARLGRRHDKRVSGRAFLRVDDD
                     REIRHALEVRHESFITPAFIELLRRERIALVVADTAGRWPFLEDVTADFVYVRLHGDV
                     KLYESGYTDDALERWSERVRAWRDGRTPPPAKLTAPRAPRRLRRDVYVYFDNDMKVHA
                     PYDAMSLAAKVEEVAHEESRRRRQPQARAVSR"
     gene            5316..6641
                     /locus_tag="VM692_06335"
     CDS             5316..6641
                     /locus_tag="VM692_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018000926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUQ51821.1"
                     /translation="MSNEAPVESVADGAPSGSVLLLGAAGLIGGAALARFRAAGRTVR
                     AVARQAGVAQDGVEWRPLDIAQCTTAAAWLPHLAGVDTVVNCAGALQGEALRAVHVDS
                     ARALYEACAQARVRRVVHLSAIGVDRESVSAFSKTKRAGEEALLESTLDWVILRPSVV
                     VGRGAFGGSALFRGLAALPVTLELPNAGPIQVVQLDELAETIVALSRPTAPARIALDV
                     AGPERLELVDVITAFRRWLRFGAAPRWRVPSWLAACGYRLGDLARALGWRPPIGNVAR
                     RELTRGAVGDSRAWQELMRIEPKSLATALAREPASVQERWFARLYFQKALAFAILALF
                     WIGTGVISLSYGWAQGLAYLLEGGVPQELAVPGVVAGALADIAIGVGIAFRRTARGAL
                     WAAVAISVFYMLAGSFVLPRLWLDPVGPMLKIWPIIVLHLVALAILEDR"
     gene            6649..>7056
                     /locus_tag="VM692_06340"
     CDS             6649..>7056
                     /locus_tag="VM692_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HUQ51822.1"
                     /translation="MTLYFLVLQYLHIIGAAVLLGTGAGIAFFMLRAHLTRDVFVIAG
                     VARIVVTADFVFTATAVVVQPVTGYLLAKFLGYPIFDGWIALSLVLYGLTGAFWIPVV
                     FMQMRMRDLAEAAVRAGTVLPPAYHRLFWTWFAF"
BASE COUNT         1062 a         2366 c         2471 g         1157 t
ORIGIN      
        1 gccgatctcg cgctcggcga gaccaaggtt cccgtgaaaa tgtactcggc cgtgcaggat
       61 gcaaaagtgc actttcgact cttgcacgcg gccgaccatg cgcccgtcaa gcagcagatg
      121 gtcgatcccg cgacggagca gcccgtggtc gcgacgcaaa cgaagaaagc cgtcgcggtc
      181 gatcgcggcg tctacgtgct gctgaccgag gaagagcagg ttgccctcgc gccgaagccg
      241 tcgcggctca tcagcatcga gcaggtcgtg ccggcggacg ccatcgacga gcgctggttc
      301 gatcggccct actacttagg gccggacggc gacgaggaag gctacttcgc gctcgcggag
      361 gcgctcgaca aacgcggcgc agtcggcgtc gcgcattggg tgttgcgcaa ccgcagctac
      421 gtcggcgcgt tgcaggcgag cggcggctat ctgctgctcg acacgttgcg ttacgccgag
      481 cagatcgtcg aggtcggcgc cgtgcagccg agcgccaatc gagcgccgga caagcgcgag
      541 atcgcgctcg ccgagcagct gatcggcgcg ctcgaggatc gtttcgatgc gacggagttc
      601 cgcgacgagt accaggagca ggtcaggaac atgatcgcgg ctaaggccgc gggcaaggtc
      661 gtgcgcttcc cgaaggcgcc gaagcgcgag cctaccgatt cgctcgtcgc gagcctgcaa
      721 gcgagcctca aggcgagccg caaggacgtt ggagcgggcc atggccgaag agcctgaagc
      781 gcctgaagcg cagccgaacc cgcgagcgtt ctggagcggc acgctgacgt tcggcctcgt
      841 cagcgtgccg gtcgacttgt atcccgcggt gcggccgcgg cgcacgccgc tgcgcatgct
      901 cggccctgaa gagcagccgc tggtccggcg ctacttctgc tcgatcgacg gcgaagcgct
      961 gacgaacgac gacatcgtgc gcggttacga gaacgacgac ggcagcttca cgatcgtcga
     1021 ggacgaagag ctcgcggcgc tggcgccgcg caagtcgcgc gacatcgacc tgcgccgctt
     1081 cgtcgcgcgc gacgagatac cgcggcagct gctcgagcgg ccgtacgtgc tcgcgcccgc
     1141 cggcgactcg acgaaggcgt atcacttgct cgcggcgacg atggagagct cggaccgcgc
     1201 cggtatcgcg acgttcgtca tgcgcggcaa ggagtacttg gccgcgatct tcgccgaagg
     1261 cggcacgctg cgcgccgtga cgatgcgttt cgtcgcggag ctgcgcacgc cgcaagacgt
     1321 cggcctgccg cgccctgcgc gagcggccga ggaacggcgc cgcgagatgg agcgcgcgct
     1381 cgccgagctg acggccgacg agctcgacac caaattgctc gaggacgact acgcgacgaa
     1441 gctgctcgag ctcgcagcgc tcaagcgcga ggccggtcaa gacgtcgtag agatcgaaga
     1501 ggcgccggcc gacgacgagg aagagggcgc cgacatcatc gacatcatga cggtgctgcg
     1561 cgagcgcatg ggcgcggcac gcggccccgc acgcgaccct ggtaagtccg cgaaggcggg
     1621 cgcggagccg ccggcgcgcg agcacggcga cgacgcgctc gcgggcaaga gcaagaagga
     1681 gctctacgag caggcgcaga agctcaagct gccgggccgc agcgcgatgt cgcgcgacga
     1741 gctgatcgcg gcgctgcgcc ccccttcggg gacttccaag ggtcgcaagg ccggctagct
     1801 agtcgacgag cgcacgccgc gccgcctcga acccttccca cggatcgcgt ttgcggcgcc
     1861 gaaccagtgc cgggacctcg agcaagccat accggggcgc ttgcgctgcc tttgcgtcga
     1921 gctcgtccca ttcgatcggc agcgccaccg gcgcgccggc gcgcgcgcgc ggcgaatagg
     1981 atccgatcgc cgtggcttct gcgtcgttgc gcaagtagtc gatgaagatc ttgccgacgc
     2041 gtttggtctt cgacatcgac gcgatgaatc gccgcggttc cgcgcgcacc atctcgttga
     2101 cgaaggcgtg cgtgaacttc ttgacggcag gccaagtcgg gcccggagtc acgggcacga
     2161 cgacgtgcaa gcctttacct ccggtcactc ttgcgaacgc attgagcccg agcgcttcga
     2221 cgcgcccttt gacgtcgaag ccggcgcgga cgacctcgtg ccagggcacg tcctcggcag
     2281 ggtcgagatc gagcacgatg atgtcgggtt ggtcgagatg ctcggcacgc gagccccaga
     2341 cgtgcatctc gagcacgccg atctgcacga gcgcttgcag cgacgcgacg tcgtcgatgg
     2401 cagcgtaggg atcttcgccc tccgtgatcg caacgcgcgg cacggccgcc ggcacgccga
     2461 caccgacgtg tttctgccag aagcactgac ctgcgtaacc ctcggggcaa cggcgcagcg
     2521 tcaacggccg gccgcgcagt agcggcaggg cgacgtcggc aacctcgcgc cagtagtccg
     2581 cgagctggcg cttcgtgatg ccggggtcgg gaaacagaat cttgtcgggg tgcgtgaggc
     2641 gcacctgcgc tttgggtccg gccgcaggag tcgcagccgg cgcgctctcg gtccgctcga
     2701 ccggcgcttc cgcccgctcc tcgacgacct cggcggccgt cttgtcttcg cgcaggccga
     2761 cgtaaatcgg atgccgcagc cggccgtcac tcgtccattc ggtataggcg atctccgcga
     2821 ccagctcggg ctcgacccag tgcacccccc gcgcgacgcc gcgtgccgac gctcgctcga
     2881 cgggcggcaa gcgccgctcg aggggacgta agcgcgcgtg cagcgcgcgc aacgtccgtt
     2941 cgtcgaagcc cgtaccgacc ttgccggcgt aacgcagcgg cgccgctgcg tcgctgcgcg
     3001 tcgcaacgag cagggcgccg aaagccgtgc gcgacccgcc ggggtccgtg aagccgacga
     3061 cgacgaactc ctcgcgcttc aaacatttga tcttgagcca actgcgcgtg cgctcctggc
     3121 gataagggtc tgcttcgcgc ttggcgatga tgccttccag cccgagcttg caggcggcgg
     3181 caaaaaattt cgcgccgttc tcggtcacgt gctcgccgaa gcggatcgcc gcgggggcgc
     3241 cttcgagcaa ctgctcgagc agccgcttgc gctcgaggag cggcacgcgc gtgagatcga
     3301 agccgtcgag atgcacgagg tcgaacgcga cgagctcgat ctgcgagctg tcgcccttca
     3361 gcgcgttttg tagccgctgg aagctggtca cgccgtgcga gtcgaagacg acggcttcgc
     3421 cgtcgaggat cgcggtgcgg cacggcaatt ttttcgcggc cgcggcaatc gccgtgaatt
     3481 cactcgtcca gtcgttaccg cgccgcgtca gcaccgtgac gtcgccgtcg tcgatgcggc
     3541 agagaacccg gtagccgtcg agcttcggct cgaacagcca accgggcccg tcgggcggtc
     3601 gcgtgaccgg cgtcgtgagc tgcggcgacg gtgtcttcgg cagcgcagcc cggcgagccg
     3661 cggggacgcc ctcgggatca ggcgcggact gcttgcgccg cacgcgaacc ttggaagtcc
     3721 ccgaaggggg gcgccccttg gaagtccccg aagggaggcg acccttgggg gtacccgacg
     3781 aagggcgcgg tgtcgcggtc ttgcgctcgg cctctggacg atccgtgcgt ttcagcagca
     3841 gccaattgtg cttgtcggat ttgcgcttgc cgcgaccctt ggaggtcccc gaagaggggc
     3901 ggtcggccat gcgcacgagc gtgaagcggc ctttgagccg ctcgccgtcg agctcgaagt
     3961 cgagcttgcc ctctttcaaa ccctcatcgg cgtcgtttag cggctgccaa tggccgcggt
     4021 cccagatcac gacgctgccg gcgccgtatt cgccctccgg gatctcgcct tcgaagccgc
     4081 cgtagtcgag cgggtgatct tcggtctgca cggcgagccg cttgtccttc gtgctcaagc
     4141 tcggctcctt cggcacggcc cagctcttca gcacgccgtc gtgctcgagg cggaagtcgt
     4201 agtgcagccg cgtggcatcg tgcttctgca cgacgaattg caacgcgcct tggccggcgg
     4261 gggccgctcg ttgactgccg ctcggttccg gcgtcttgtt gaagtggcgc ttagcgcgat
     4321 acggcgcgag cttgcggctg gcgccgccgg cggctttctt catgggcaac ctcctcgact
     4381 tttgcggcca gactcatggc gtcgtacggc gcgtgcactt tcatgtcgtt gtcgaagtac
     4441 acgtaaacgt cgcggcgcag acgtcgcggc gcgcgcggcg ccgtcaactt agcgggaggc
     4501 ggagtgcgtc catcgcgcca ggcgcgtacg cgctccgacc agcgctcgag cgcgtcgtcc
     4561 gtgtagccgc tctcgtagag cttgacgtcg ccatgcagcc gcacgtaaac gaaatcggcc
     4621 gtgacgtcct cgaggaacgg ccagcggccc gccgtgtcgg cgacgacgag cgcgatccgc
     4681 tctctgcgca gcagctcgat gaacgcgggc gtgatgaagc tctcgtgccg gacctcgagc
     4741 gcgtggcgaa tctcgcggtc gtcatcgacg cgcaggaacg cgcggcccga gacgcgcttg
     4801 tcatggcgcc ggccaagccg cgccgcttcc ttcgtcgacc gcggcagcag cgcgaaaaac
     4861 tcggcgagcc gcgcttcgtc aaacttgaac gtcggcggca gctgccacag cagcgggccg
     4921 agcttgtgcc gcagcttcag caccccggaa gcgaagaaat ttgcgagggc cgtcgcgacg
     4981 cctttcagcc gcagcatgtg cgtgatgtag cgcgagcctt tcacggcgaa gacgaagctc
     5041 gccggcactt ggtcgtacca gcactcgtag tactcggggc gctgcagcga atagaagctg
     5101 ccgttgagct cgatcgtggg gaagtgggcc gccgcgtagg caagctcctc gcgttgcggc
     5161 aatccggccg gatagaagtc gccgcgccac ggcgcgtaac gccaacccga gatcccgatc
     5221 agtgcgcgcg tgctcatggg gccatggcga tgcaaacgat atgccagtcg cggcatcgcg
     5281 gcggctcgtg gcacactctt tgctctggag ccacgatgtc gaatgaagcc cccgtcgagt
     5341 cggtcgccga cggcgcaccc tcaggatcgg tcttactgct cggcgccgcg gggctgatcg
     5401 gcggcgctgc gctcgcgcgc tttcgtgcgg ccggccgcac cgtgcgagcc gttgcacggc
     5461 aggccggcgt tgcgcaggac ggcgtcgagt ggcgcccgct cgatattgcg cagtgcacga
     5521 ccgcggcggc ttggctgccg cacctcgctg gtgtcgatac cgtcgtcaat tgcgccggcg
     5581 cgctgcaggg cgaggcgctg cgtgccgtgc acgtcgatag cgctcgcgcg ctgtatgaag
     5641 cctgcgcgca agcccgagtg cgacgcgtgg tgcacctgtc ggcgatcggc gtcgatcgcg
     5701 aatccgtcag cgcgttctcg aagacgaagc gtgccggcga ggaggcgctg ctcgagagca
     5761 cactcgactg ggtcatcctg cggccgtcgg tcgtcgtcgg gcgcggcgca ttcggcggca
     5821 gtgcgctgtt tcgcgggctg gccgcgttgc ccgtgacgct cgagctgccg aacgcggggc
     5881 ccatccaagt cgtgcagctc gacgagctcg ccgagacgat cgtcgcgttg agccggccga
     5941 cggcgccggc gcgcatcgcg ctcgacgtgg cagggccgga gcgcctcgag ctcgtcgacg
     6001 tcatcacggc gttccgtcgc tggctgcgct tcggcgctgc gccgcgctgg cgagtgccga
     6061 gctggctcgc ggcgtgcggc tatcggctcg gcgacctcgc gcgcgcgctc ggctggcggc
     6121 cgccgatcgg caacgtcgcg cggcgcgagc tcacgcgcgg cgccgtcggc gactcacgcg
     6181 cgtggcagga gctcatgcgc atcgagccga agtcattggc gacggcgctc gcgcgcgagc
     6241 ccgcatccgt gcaggaacgg tggttcgcac ggctgtactt ccagaaggcg ctcgcgttcg
     6301 cgatcctcgc gctgttctgg atcggcacgg gcgtcatctc gctgagctat ggttgggcgc
     6361 aaggcttggc gtacttgctc gagggcggcg ttccacagga gcttgccgtc cccggcgtcg
     6421 ttgcgggggc gcttgccgat atcgcgatcg gagtcggcat cgcattcagg cgcacggcgc
     6481 gcggcgcgct gtgggccgcc gtggcaatct ccgtgttcta catgctcgcg ggaagcttcg
     6541 tgctgccgcg tttgtggctc gaccccgtcg ggccgatgct gaagatctgg ccgatcatcg
     6601 tgctgcatct cgtggcgctc gccatcctcg aggaccgttg agacgccaat gacgctgtac
     6661 tttctcgtgt tgcagtacct gcacattatc ggcgccgccg tcctgctcgg gaccggcgcg
     6721 ggcatcgcgt tcttcatgct gcgcgcgcat ctgacgcgcg acgtgttcgt gatcgccggc
     6781 gtcgctcgga tcgtcgtgac ggccgacttc gtgttcacgg caacggccgt cgtcgtgcag
     6841 cccgtcacgg gctacctgtt ggcgaagttc ttgggttacc cgatcttcga cggctggatc
     6901 gcgctgtcgc tcgtgctcta cggcctcacg ggcgcgttct ggattcccgt cgtcttcatg
     6961 cagatgcgca tgcgggattt agccgaagcc gccgtgcgcg cggggacggt gctgccgccc
     7021 gcgtatcacc ggctcttctg gacgtggttc gcgttc
//
LOCUS       DATLEE010000300         5641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10609125, whole genome shotgun sequence.
ACCESSION   DATLEE010000300 DATLEE010000000
VERSION     DATLEE010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10609125"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VM692_06345"
     CDS             <1..267
                     /locus_tag="VM692_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51823.1"
                     /translation="IGDAFVVVGLALRHQPSGLEIDLGGPVYFPAAETASHHGVDGAR
                     VLHVAWRPGARRERPGGERARACILNTGVDSEASGAVRRPIDGG"
     gene            272..2371
                     /locus_tag="VM692_06350"
     CDS             272..2371
                     /locus_tag="VM692_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HUQ51824.1"
                     /translation="MGTKVRPNTAAWLATALSLSGAAAYAQAPAIDNDDLGGTVNGPS
                     GTEAGVWVIAETDAFQTRYAKIVVTDDQGRYVIPDLPPADYRVWVRGYGLVDSTKVTG
                     RPGQTLNLVANAAPSPAAAAVVYPAAYWYSMMKVPPAAEVSSLPGGVNQYLASVKNLS
                     CIGCHQIGQLATRTLPPPYASLPSHHEAWVRRIQSGQAGSQMLASAMNQLGGVPPKYL
                     ADWTERVAKGELPHAQPQRPHGVERNVVATIRDWATDKAYMHDLSGTDRRNPTVNGYG
                     KLYGSPELSTDDFPILDPQTNTATSHHAPVQDPHTPSTHETPPTQPSAYWGSEAIWDS
                     KANAHNPMLDAQGRVWYTAVVRAPRNAPAYCKADSGHPSAKLFPLEQSGRQLSVYEPK
                     TGKYTFIDTCYSTHHLQFDANDILWTSGGGPVVGWLDAKKFLETGDAGKSQGWTALVL
                     DTNGNGKRDEYTEPNQPADPAKDRRILSPFYAVMPNPADGSVWGSTLGYPGAVVRVNP
                     GANPPATALAEVFNVPMPGYGARGADIDSNGVVWVSLGSGHLGAFDRRKCKGPLNGPT
                     ATGDHCPEGWSFHRYPGPGFIGRPDESVESSYYSWVDQHNTAGLGDNVPMSTANLFDG
                     VHALVNNEMVTLRIPYPLGFYAKGFEGRIDDANAGWKGRGLWVPSGDRTPWLKEGGKG
                     TKPLVVHFQVRPNPLAK"
     gene            2462..2758
                     /locus_tag="VM692_06355"
     CDS             2462..2758
                     /locus_tag="VM692_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HUQ51825.1"
                     /translation="MKKIVVASALAAAAGWAFAQPPGGFTPPSFAGLDTDKSGGISQA
                     EVAAFFANAPANANRPPPEQIFANWDANKDGSVSQAEFDARPRPQGPGGPPPAR"
     gene            complement(2841..5348)
                     /locus_tag="VM692_06360"
     CDS             complement(2841..5348)
                     /locus_tag="VM692_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ51826.1"
                     /translation="MLLAVSLASAKIVWAQPQPPLVGRTVQGVLDELRAAGAPFVYSS
                     NLLPSTLTVTAEPNATDELALAREILQPHGLAVRVESGVWLVVRGEPPASPAPFAPGT
                     IVLRAQAGYAGGRIQAPDVQLDPPTGPTASATDDGAIRFEGVAPGRHRLLVSAAGFVA
                     ERFAVEVAPGRISERTVALFEAVPKLEEVVVTASRYEVGARTEPSATYLSRDDIETVA
                     SLAGDTIRVVHHLPGIASNEFSARPYVRGGAANELAVLIDGIRLIEPYHLRDFQGVFS
                     VVDQRIVDSVAVHAGGFPAAYGDALSALLIIEPREPTQRDTEIGLSALYTSGLSSGTF
                     ADGRASWLVSARNSNLDRVLADHIGDPAYSDLFWRVTADLGERHRLAAGKLRFRDDIV
                     LTLENEPTDRQVATSDTNSRQSWLKLDSQWTDALSSSTWLYGTTFESSRREDVADLAE
                     IVGNVDDHRELEVVGLKQAWQFEPSMRQLWTFGAEVEEREARFRYASAADRRGLLATL
                     GNRAPPLRMTALSPSGNSYGWYVEDRVRVTDRLIADLGVRWDRQTYVPGNDSQFSPRA
                     SMLYRLGSKTDLRLSHGRFFQAEGLLELQVEDGVTEFYAPQRAGHSILSVERRFTGSL
                     ALRVELYRKWTRNVRPHYENLFDPLELIPELRASRVLVAPDRAESEGLEVFLSGEEPV
                     SWWAGFTSARVQDLIDGVPVSRSWDQRRALTAGATWPVGGWSLSAAATMHRGWPTTRV
                     TVVTGSTGDRVAVAGPRNSDRLGGVRRLDVRASREFGVGPGSLRFFAEVTNLTNRQNA
                     CCLVYDPVTLPSGLPSLVGIERARAGITGNIGLLWQF"
     gene            complement(5454..>5641)
                     /locus_tag="VM692_06365"
     CDS             complement(5454..>5641)
                     /locus_tag="VM692_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51827.1"
                     /translation="WFSRQTGRSVVFGSDAAERLARETRLSGSIDLAPLQKLSAVLTL
                     TDLTYALEDERVVIETR"
BASE COUNT          994 a         1897 c         1863 g          887 t
ORIGIN      
        1 atcggcgacg cgttcgtcgt tgtcggactg gcattgaggc accaaccttc gggcctcgaa
       61 atcgatctcg gcgggccggt gtattttccg gcggcggaaa ccgcgagtca ccacggcgtg
      121 gacggcgcgc gcgtgctgca cgtcgcgtgg cgcccgggcg cgcgtcgaga acgcccgggc
      181 ggggaacggg cacgcgcttg cattctcaat accggcgtgg atagtgaagc gagcggcgcc
      241 gtccggcgcc cgattgacgg gggatgacac catggggacg aaggtacggc cgaatacggc
      301 cgcgtggctc gcgacggctc tctcgctctc tggcgcggcc gcctacgcac aggcgccggc
      361 catcgacaac gacgacctcg gcggcaccgt caacggtcca agcggcaccg aagccggcgt
      421 ttgggtcatc gcggagaccg acgcattcca gactcgttac gcgaagatcg tcgtcaccga
      481 cgaccaaggc cgctacgtca ttcccgatct gccgcccgcc gactacagag tctgggtgcg
      541 tggctacggc ctcgtcgatt cgacgaaggt cacgggtcgc cccggccaaa ctttgaatct
      601 cgtcgcgaac gccgctccgt cgccggccgc cgcggccgtc gtctatccgg ccgcgtattg
      661 gtactcgatg atgaaggtgc cgccggcggc ggaggtcagc tccctgccgg gcggcgtcaa
      721 ccagtacctc gcgtcggtca agaatctgtc gtgcatcggc tgtcatcaaa tcggccagct
      781 cgcgacgcgc actctgccgc cgccgtacgc gagcctgccg agccatcatg aagcgtgggt
      841 ccggcgcatt cagtccgggc aagccggcag ccagatgctg gcgtccgcga tgaaccagct
      901 cggcggcgtg ccgccgaagt acctcgcgga ctggaccgag cgcgtcgcga agggcgagct
      961 gccgcacgcg cagccgcagc ggccgcacgg cgtcgagcgc aacgtcgtcg cgacaatccg
     1021 cgactgggcc accgacaaag cctacatgca cgacttgagc ggcacggatc gccgcaatcc
     1081 gaccgtcaac ggctacggca agctgtacgg ctcgccggag ctgtcgacgg acgacttccc
     1141 gatcctcgac ccgcagacga acacggcgac ctcgcatcac gcgccggtcc aagatccgca
     1201 cacgccgtcg acgcacgaga cgccgccgac gcaaccgtcg gcgtactggg gcagcgaagc
     1261 gatctgggac agcaaagcaa acgcgcacaa tccgatgctc gatgcgcaag gccgcgtttg
     1321 gtacacggcc gtcgtgcgcg cgccgcgcaa tgcgccggcc tattgcaagg ccgactcggg
     1381 ccatccgtcc gcgaagctgt tcccgctcga gcaatccggt cggcaacttt cggtgtacga
     1441 gccgaagacc ggcaagtaca cgttcatcga cacgtgctac tcgacgcatc acctgcagtt
     1501 cgacgcgaac gacattttgt ggacgagcgg cggcggcccc gtcgtcggtt ggctcgacgc
     1561 gaagaagttt ctcgagaccg gcgacgccgg caaatcgcag ggctggacgg cgctcgtgct
     1621 cgacacgaac ggcaacggca agcgcgacga gtacacggag ccgaatcaac ccgcggatcc
     1681 cgcgaaagac cgccgcatct tgagtccgtt ctatgccgtg atgccgaacc cggccgacgg
     1741 ctcggtctgg ggctcgacgc tgggttatcc cggcgcggtc gtgcgcgtga atcccggcgc
     1801 gaatccgccg gcaacggcac tcgccgaggt gttcaacgtg ccgatgccgg gctacggcgc
     1861 gcgcggcgcc gacatcgact cgaacggcgt tgtctgggtg tcgctcggca gcgggcactt
     1921 gggcgcattc gaccggcgca agtgcaaagg cccgttgaac gggccgacgg cgaccggcga
     1981 ccactgcccc gaaggttgga gcttccaccg ctatcccggc ccgggattca tcggccgtcc
     2041 tgacgagagc gtcgagtcga gctattacag ctgggtcgat cagcacaaca cggcgggtct
     2101 tggcgacaac gtgccgatgt cgacggcgaa cctgttcgac ggcgtgcacg cgcttgtcaa
     2161 caacgagatg gtgacgctgc ggattccgta tccgctcggc ttctacgcga agggcttcga
     2221 aggccgcatc gacgatgcaa acgcgggttg gaaaggccgc ggcctatggg tgccgagcgg
     2281 cgaccgcacg ccgtggctga aggaaggcgg caagggcacg aagccgctcg tcgtgcactt
     2341 ccaagtccgg cctaatccgt tggcgaaata gatacttaca gtcagcgact tgaagtgaat
     2401 tctagggagt cccctccgag gagggggcct tctgttcagc tagccaccgg gggattcatc
     2461 gatgaagaag atcgtcgtcg catcggcact cgcggccgct gcgggatggg cattcgcgca
     2521 accgcccggc ggctttacgc cgccatcgtt cgcgggcctc gacaccgaca agagcggcgg
     2581 gatttcgcaa gcggaggtcg cggcgttctt cgccaacgca ccggcgaacg cgaaccggcc
     2641 gccgcccgag cagattttcg cgaattggga cgcaaacaag gacggctccg tctcgcaagc
     2701 cgagttcgac gcacggccgc ggccccaagg gccgggcggt ccaccgccgg cgcgctgacc
     2761 ttcccgtctc gttgaccccg cgcgtcaacc gcgcttcgtg gcgctggggc gcccggtttc
     2821 ctcgctgtag cgccgcgtag tcagaactgc caaagcagcc cgatgttgcc ggtgattccg
     2881 gcgcgagcgc gctcgatgcc gacgagcgac ggcagtccgc tcggcaaggt cacggggtca
     2941 tacacgaggc agcacgcgtt ttgccgattc gtcaaattcg tgacttcggc gaagaaccgt
     3001 aatgagccgg gcccgacgcc gaactcgcgg ctcgcgcgaa catcgagccg ccgcacgccg
     3061 ccaagccggt cactgttgcg tggccccgca acggcgacac gatcgcccgt cgaccctgtt
     3121 acgaccgtca cccgtgtcgt cggccagcct cggtgcatgg tggccgccgc gctcaggctc
     3181 catccgccca cgggccaagt cgcgccggct gtcagagcgc ggcgttggtc ccagctgcgc
     3241 gacacgggca cgccgtcgat caggtcttgg actcgggccg acgtaaagcc ggcccaccac
     3301 gagacgggct cctcgccgct gaggaaaacc tcgagccctt ccgactcggc acgatcgggt
     3361 gctactagaa cacggctcgc acgcaattcc ggtatcagct ccaaggggtc gaacaaattt
     3421 tcgtaatgcg ggcgaacatt tcgcgtccac ttccggtaga gctcgacgcg gagcgccaag
     3481 ctgcccgtga atcggcgttc gacgctgagt atcgaatgac ccgcgcgttg cggcgcgtag
     3541 aactcagtta cgccgtcttc gacctgcagc tcgagcagcc cttcggcttg gaagaaccgg
     3601 ccgtgactca gacgtaagtc cgtcttgctg ccgagccgat agagcatgct cgcgcgcggg
     3661 ctgaattggc tgtcgttgcc gggcacgtaa gtttgccggt cccagcgcac gccgagatcg
     3721 gcgatgagtc gatcggtgac gcgcacgcgg tcttcgacgt accagccgta actgttcccg
     3781 ctcggcgaga gcgcggtcat gcgcaacggc ggcgccctgt tgcccagcgt ggcgagcaag
     3841 ccgcgacggt cggcggcgct ggcgtagcgg aagcgtgctt cacgctcttc gacctccgcc
     3901 ccgaacgtcc agagctggcg catcgacggc tcgaattgcc acgcttgctt gagcccaacg
     3961 acctcgagct cgcggtggtc gtcgacgttg ccgacgatct cggccagatc ggccacgtcc
     4021 tcgcgtcgcg acgactcgaa ggtcgtgccg taaagccacg tcgagctcga caacgcatcg
     4081 gtccactgac tatcgagctt gagccaggat tgccggctgt tggtatcgct cgtcgcaacc
     4141 tgccggtcgg tgggctcgtt ctccagagtc agcacgatgt cgtcacgaaa ccgcagcttg
     4201 ccggccgcga gccggtggcg ttcgccgagg tcggccgtca cacgccaaaa caagtccgag
     4261 tacgccgggt cgccaatatg atcggcaagt acgcgatcga ggttgctgtt gcgcgccgat
     4321 acgagccagg atgcgcggcc gtccgcgaat gttccgctcg acagccccga cgtgtacaac
     4381 gcactgaggc cgatctcggt gtcgcgttgc gtcggctctc gcggctcgat gatgagcaag
     4441 gcgctcaacg cgtcgccgta agccgccggg aagccgccgg cgtgcacggc gacgctgtcg
     4501 acgatgcgtt gatcgacgac gctgaatacg ccctggaagt cgcgaagatg atacggctcg
     4561 atgaggcgaa taccgtcgat gagcacggcc aactcgttcg ccgcgcctcc gcgcacgtac
     4621 ggcctcgccg agaactcatt cgacgcaatt cccggcagat gatggacgac gcggatcgta
     4681 tcgccggcca acgaggcaac ggtctcgatg tcgtcgcgcg acaggtaggt cgccgagggt
     4741 tcagtgcgtg cgccgacctc gtagcggctc gcggtcacga cgacttcttc gagcttgggc
     4801 acggcctcga agagcgcgac cgtcctctcg gaaattcgcc ctggcgccac ttcgacggcg
     4861 aagcgctcgg ccacgaaccc agcggcgctc acgagcaatc tgtgccgtcc gggagcgacg
     4921 ccttcgaagc ggatcgctcc gtcgtcggta gccgaggcag tcggtccggt tggagggtcc
     4981 aattggacgt caggagcttg aatacgccca ccggcataac cggcctgcgc gcgtagcacg
     5041 atcgttcccg gcgcgaacgg cgcgggcgac gcgggcggct cgccgcggac gacgagccag
     5101 acgccggact ccacgcgcac ggcaagcccg tgcggctgca atatttcccg cgcgagcgcc
     5161 agttcgtccg tggcgtttgg ctccgcggtc accgtgagcg tcgaaggaag caggttgctg
     5221 ctgtagacga agggcgcgcc ggccgctcgc aattcgtcga ggacaccctg caccgtccgc
     5281 cccactagcg ggggctgcgg ctgagcccac acgatcttcg cgctcgccag tgaaacggcg
     5341 agcagcacga cgataggaca acgggacgcc atcgaccacc gaacacgcag gttctgcggt
     5401 ccgattgtac gcaatgaccc gcgcaggcgc cgacgcgacg ggcagcgagc tcgctagcgc
     5461 gtctcgatta cgacgcgttc atcctcgagg gcgtacgtaa gatcggttag cgtcaatacc
     5521 gccgacaact tttgcagcgg cgcaaggtcg atcgagccgc taagtcgcgt ttcgcgggcc
     5581 aagcgttcgg ccgcgtcgct gccgaacacg acgctgcgtc ccgtttggcg ggagaaccag
     5641 g
//
LOCUS       DATLEE010000301         4342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9284709, whole genome shotgun sequence.
ACCESSION   DATLEE010000301 DATLEE010000000
VERSION     DATLEE010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9284709"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VM692_06370"
     CDS             <1..219
                     /locus_tag="VM692_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HUQ51828.1"
                     /translation="GFLGAGAILKVESERSIHGLTTAAGIWMTAALGVAIGLGLLGTA
                     VVGALFAWAVLTFLIRLEKHREGSSNRI"
     gene            283..852
                     /locus_tag="VM692_06375"
     CDS             283..852
                     /locus_tag="VM692_06375"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HUQ51829.1"
                     /translation="MRAITSLPIAALTAALTLGAAAQSRAPAPPLPDRSIVTAEGPRE
                     TASSNKNTEDVEFLTDAMRTALAEVQLGELATQRSSQQRVRDYGEKLKSDHAAQATEI
                     ERMLKPLNVAIPQEPGAEALGHRAALERLSGQEFDSAFIEMMIASHREAIDKFGAQTH
                     ANPNRALAEFASKSLATLREHLALAEALR"
     gene            870..1253
                     /locus_tag="VM692_06380"
     CDS             870..1253
                     /locus_tag="VM692_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HUQ51830.1"
                     /translation="MSKSPGHRQHPEHRVRERALGARVRATVGDDVIADSGDVIKVEE
                     DGNPPRYYFPRADVKMDRLAPSATTSQCPFKGQATYFDVSSNGRRLKDAVWSYEEPYD
                     EHAELKERVAFWDDKLPEIEISPRL"
     gene            1404..3338
                     /locus_tag="VM692_06385"
     CDS             1404..3338
                     /locus_tag="VM692_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HUQ51831.1"
                     /translation="MLAAVTVAARADEASDAALREQFRVAYAAVDVALAPDDDALRAY
                     VLYPYLRAARIARALERATGTQSQPDLEAAEFLAETGDAPVARALRRAWLASLARRNS
                     WDAFLERYDDSVATPLLQCQRLNARIARAATDGLAAEIRSRWLTPYRLPTECEPAFQW
                     LRAQNELPDELIAQRATRLLDAGQASFARIVAARLPRDAAAPLLERADFIESPGRMLD
                     ALLRDPARAVPGNVVLEAWSRLARNAPDAALARFGDLTERTATAVQPHELALALALGL
                     AWDRRAEALDYFARVPATALDDADREWQTRAALWAGDWESVRATIAAMPAAQQSEWAW
                     RYWAARAVEQRGDGAAARTLYAAVLDGDNYYSALAAAHLGERVVPRVEPLPLDPATVD
                     TIAALDAFRRVRELLLVGQRQLARTEWNYGYELLPDEQRLQAIHLAARLGIYDIAVET
                     ATSYGQFNDYPLLYPRPYADEVAAAVKLTDVEPPLLYGVLRQESLFRPDAASSAGALG
                     VAQLTHATARETARRWRLPAPKREDLFDPAVNITLGAARFAELLEQFDAQLPLALGAY
                     NAGTAAVERWLPAQPIDSDVWIENIPYNETRAYVRRVLWHSLVFSWLESGRAQSARDW
                     LGKVERPRKSPAAESTSQHR"
     gene            complement(3343..>4342)
                     /locus_tag="VM692_06390"
     CDS             complement(3343..>4342)
                     /locus_tag="VM692_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUQ51832.1"
                     /translation="LRAGAHVVVTTRFPVDAASRYAKEHDFAELRARLQIFGLDLRHT
                     PSVDLFTRFLSERLPRLDYIVNNACQTVRRPAGFFRHLLEAEANPIAALPAELRSVLA
                     EHGELCRVLEEPQERGTRSLAAAATGRGEGVRHSAALSQRRYLDDDYRAGEALFPAAR
                     YDEDLQQVDLRDTNSWRLRLHEVATPELLEVHLVNAIAPYILNARLKPLLVATPSRHK
                     HIVNVSAMEGQFYRTTKTDKHPHTNMAKAALNMMTRTSAPDYVKDGIHMNAVDTGWVT
                     DEDPAAHAARKAELGFSPPLDIIDGAARIVDPIFVGRLTGDHVWGQFLKDYKPAPW"
BASE COUNT          698 a         1455 c         1488 g          701 t
ORIGIN      
        1 ggcttcctcg gcgcgggcgc catcctaaaa gtcgagagcg agcgctcgat tcacggactc
       61 acgaccgcag ccggtatttg gatgacggcg gcgctcggcg tcgcgatcgg tttgggcctc
      121 ttgggcacgg ctgtcgtggg cgcgctgttc gcttgggccg tgctgacctt tttgattcgg
      181 ctcgaaaaac accgcgaggg ctcgagcaat cgcatttgac gggctccgcc gagctggtat
      241 gcgtcttgca gcggcagggc ccacccgaaa aggagttcgg acatgcgagc catcacttcc
      301 ttgccgatcg cggcgctgac cgcggctttg actctcggcg cggccgccca aagtcgcgcg
      361 ccggcgccac ccctacccga tcgcagcatc gtcacggcgg aagggccgcg cgagacagca
      421 tcgagcaaca aaaacaccga ggacgtcgag ttcttgaccg acgcgatgcg aacggcgttg
      481 gccgaggtgc agctcggcga gctcgcgacg cagcgcagct ctcagcagag agtgcgcgac
      541 tacggcgaga agctcaaaag cgatcacgcg gcgcaggcca ccgagatcga gcggatgctg
      601 aagcccctga acgttgcgat tccgcaagag ccgggcgcgg aagcgctggg ccaccgcgcc
      661 gcgctcgagc gcctgtcggg gcaagagttc gattcggcgt tcatcgaaat gatgattgct
      721 tcgcacaggg aggcgatcga caagttcggc gcgcaaacgc acgcgaatcc gaaccgggcg
      781 ttggcggagt tcgcatcgaa gagcctggcc acgctgcgcg agcacttggc tctcgcagaa
      841 gcactgcgct gatgcagcag gagacgatca tgagcaaatc accgggccat cgtcagcatc
      901 cagagcaccg agtccgcgag cgcgcccttg gcgcacgcgt ccgtgctacc gtcggcgacg
      961 atgtgatcgc cgactccggc gacgtcatca aggtcgagga agacggcaac ccgcctcgtt
     1021 attacttccc gcgcgccgac gtgaagatgg accgcctcgc gccttccgcg accacgtcgc
     1081 agtgcccctt caaagggcaa gccacctact tcgacgtcag cagcaacggg cgcaggctca
     1141 aggacgccgt ctggagctac gaagagccgt acgacgagca cgcggagctc aaggagcgcg
     1201 tggcgttctg ggacgacaag ctgccggaga tcgagatcag cccgcgactc tgacggcttc
     1261 ggccgccggc tggccctgca gcgccaccgt tcgagcgtgc gaaatttgcc gtgtatcctt
     1321 cgcgcatgcc gtcggagcga gctcattgcc gtgcccagcg ccgcttgtgg cgtggccgcg
     1381 tagcgtgcgg cttggccgcg tgcatgctgg ccgccgtcac ggtggcggcg cgcgccgatg
     1441 aagccagcga cgccgcgcta cgcgagcagt ttcgcgtggc ctatgccgcc gtcgacgtcg
     1501 cccttgcgcc tgacgacgac gcgctgcgcg cgtacgtgct gtatccgtat ttgcgcgccg
     1561 cgcgtatcgc ccgagcgctc gagcgcgcaa cgggcacgca gagccaaccc gatctcgaag
     1621 ccgccgagtt tcttgcagag acgggcgacg cgccggtagc gcgcgccttg cgccgtgcat
     1681 ggcttgctag cctcgcacgc cgtaactcgt gggacgcatt cctcgagcgt tacgacgact
     1741 ccgtggccac gccgttgttg cagtgtcagc gtctcaacgc tcgcatcgcg cgcgcggcga
     1801 cggatgggct ggcggccgag atccgctcgc ggtggctgac gccgtatcgg ctgccgaccg
     1861 aatgcgagcc tgcatttcaa tggctacgcg cgcaaaacga gttgcccgac gagctcatcg
     1921 cgcagcgcgc aacccggctg ctcgatgcgg gtcaagcgtc ttttgcgcgc atcgtcgcgg
     1981 cgcgtttgcc gcgcgacgcc gccgcgccgt tgctcgagcg cgcagacttc atcgagagcc
     2041 cggggcgcat gctcgacgcg ctgcttcgcg atccggcgcg agccgttcca ggtaatgtcg
     2101 tgctcgaagc atggtcgcgg ctcgcgcgta acgcgccaga cgccgcgctg gctcgcttcg
     2161 gcgacctcac cgagcgcacg gcgacagccg tgcaaccgca cgagctggcg cttgccctgg
     2221 cgctcggtct cgcgtgggac cgacgcgccg aggcgctcga ttatttcgcg cgcgtgccgg
     2281 ctaccgcgct cgacgacgcg gaccgcgagt ggcaaacgcg tgcggccctg tgggcaggcg
     2341 attgggagtc ggtgcgcgcg acgatcgccg cgatgcctgc ggcgcaacag tccgaatggg
     2401 catggcgtta ctgggccgcg cgcgcggtcg agcagcgcgg cgacggcgcc gcggcgcgca
     2461 cgctctatgc ggcggtgctc gacggcgaca actactactc ggcgttggct gcggctcatc
     2521 tcggcgagcg cgtcgtgccg cgcgtcgagc ctctgccgct cgacccagcg acggtcgaca
     2581 cgatcgcggc gctcgacgcc ttccggcgcg tgcgtgagct cttgctcgta ggccagcggc
     2641 agctcgcgag aacggaatgg aattacggct acgagctgct gcccgacgag cagcggctgc
     2701 aggcgattca cctcgccgcg cggttgggaa tctacgacat cgccgtcgag acggcgacga
     2761 gctacgggca gttcaacgat tatccgctgc tctacccgcg gccttatgcc gacgaggtcg
     2821 cggcggccgt caaattgacg gacgtcgaac cgccattgct ctacggcgtt ctgcggcaag
     2881 aaagcctgtt ccgtcccgat gccgcgtctt cggccggcgc actcggcgtc gcgcagctca
     2941 cgcatgcgac ggcacgcgag acagctcgcc gctggcggct gccggcgccg aagcgtgaag
     3001 acctgttcga tccggccgtc aacatcacac tgggtgccgc acgattcgcg gagctactcg
     3061 agcagttcga cgcgcaactg ccgctggcgc tcggcgcgta caacgccggc acggccgccg
     3121 tcgaacggtg gttgccggcg caaccgatcg atagcgacgt ctggatcgag aacatccctt
     3181 acaacgagac gcgggcttac gtgcgccgcg tcctctggca cagcctcgtg ttcagctggc
     3241 tcgagtccgg gcgcgcgcag agcgcgcgcg attggctcgg taaagtcgag cggccgcgca
     3301 agagcccagc cgcggagtcg acgagccagc atcgctagcg cgtcaccatg gggccggctt
     3361 gtagtccttc aaaaactgac cccaaacgtg gtcaccggtt agccggccga cgaagatggg
     3421 gtcgacgatg cgtgccgcgc cgtcgatgat gtcgagcggc ggcgagaagc cgagctcggc
     3481 cttgcgcgcc gcgtgcgcgg cgggatcctc gtcggtgacc cagcccgtat cgacggcgtt
     3541 catgtggatg ccgtccttca cgtagtcggg cgcactcgtg cgcgtcatca tgttcagggc
     3601 ggcctttgcc atgttcgtgt gcgggtgctt gtcggttttc gtcgtgcggt agaactgccc
     3661 ttccatcgcg ctgacgttca cgatgtgctt gtgacggctc ggcgtggcga cgagcagcgg
     3721 cttgagcctc gcgttcaaga tgtaaggcgc gatcgcgttg acgaggtgca cctctagcag
     3781 ctcgggtgtc gcgacctcat gcagccgcag acgccaactg ttggtgtcgc gcagatcgac
     3841 ctgctgtaga tcctcgtcgt agcgagccgc ggggaacagc gcttcgccgg cccgatagtc
     3901 gtcgtcgaga tagcgccgct gcgacagcgc cgcgctgtgc cggacaccct cgccgcggcc
     3961 cgtcgcggcc gctgccagcg atcgcgtccc gcgctcctgc ggctcttcga gcactcggca
     4021 aagctcgccg tgctcggcga gcacgctacg caattcagcg ggcagcgcgg cgatcggatt
     4081 ggcctcggct tcgagcagat gccgaaagaa gcccgccggc cggcgcacgg tctgacacgc
     4141 gttgttgacg atgtagtcga gccgcggcag ccgctcgctc aagaaccgcg tgaacagatc
     4201 gacgctcggc gtgtggcgga gatcgagccc gaagatctgc aggcgcgcgc ggagctcggc
     4261 gaaatcgtgc tccttcgcgt agcggctcgc ggcgtccacg ggaaagcgag tcgtgacgac
     4321 gacgtgggcg ccggcgcgca gc
//
LOCUS       DATLEE010000302         2854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5570699, whole genome shotgun sequence.
ACCESSION   DATLEE010000302 DATLEE010000000
VERSION     DATLEE010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5570699"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VM692_06395"
     CDS             complement(<1..625)
                     /locus_tag="VM692_06395"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ51833.1"
                     /translation="MPRRTFLRGIGATIALPLLDAMVPAMAATPTAQSRYSFLHVPHG
                     AAPGYWRPTGSGKSWELSRILQPVAAFKDRITIISGTDHRMATSLSPEESAGDHSRTA
                     AVYLSAAHPKRTEGQDIEAGITIDQVLANKIGQQSPLPSLEMCIEDVGSLGVCGVGYS
                     CAYTNSISWSSPTSPLPMERNPQVVFERLFGDGGTPEERAARKRADRS"
     gene            complement(661..>2854)
                     /locus_tag="VM692_06400"
     CDS             complement(661..>2854)
                     /locus_tag="VM692_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ51834.1"
                     /translation="VADDRETWEKVVRKLGAGMMPPASEERPDPNKKAEFLTYLETEL
                     DRLAAAEPNPGRPALHRVNRTEYANAVRDLLALEIDATALLPADDSSFGFDNIAGSLG
                     VSPVLLERYVAAAGKISRLAIGDTTMPDRQEKYLVSGGLTQNQHLPGMPFGTRGGATF
                     THQFPVDAEYVIRADLVERGGRMFGANAGKTEQLEVTLDGQRVLLAELSDYKVEDGAA
                     IRMPVKAGPHTIGAAFIKKNHAPVEDVIQPFEFSLFEPAVDGDPDWTFVPHLASMSVT
                     GPFDIAGVSDTPTRQKIFVCRPTTAREEMPCARQIVETLATRAYRHPVDREQLSTLMD
                     FYADGREQGDFEAGIELALRRILASPEFVFRFERADDAAPGAVHRLSDLELASRLSFF
                     LWSSIPDDELLKLAIDDKLHEPATLRRQVRRMLDDTRSNAFIENFAGQWLYLRNLETK
                     GGAVEQFPNFDDNLRQAFRTETEMLFASIVREDRNLLDLLTADYTFVNDRLARHYGME
                     GIYGSEFRRVPVANEARRGLLGQGSILLVTSLPERTSPVQRGVWVLENIVGAPVPTPP
                     PVVPALEEQAGTKTHPRTVREQMRLHSERPFCAGCHKIMDPVGFALENFDAIGQWRTE
                     EHGTPIDATGRLVDGTQINGAVDLRNALLKYSDRFVQTTTEKLMTYALGRGLEYYDMP
                     TVRAIARGAAKNDYRFSSIILGIVESPAFQMRAAEEGPAGAEVVADTE"
BASE COUNT          425 a          923 c          995 g          511 t
ORIGIN      
        1 tgctgcggtc ggcgcgtttg cgcgcggcgc gctcctcggg cgtgccgccg tcgccgaaca
       61 ggcgctcgaa tacgacctgc ggattgcgct ccatcggtag cggcgaggtc ggcgacgacc
      121 acgagatcga gttcgtgtaa gcgcagctgt aaccgacgcc gcagacgccg agcgagccga
      181 cgtcctcgat gcacatctcg agcgagggca gggggctttg ctggccgatc ttgttggcaa
      241 gcacctggtc gatcgtgatg ccggcctcga tgtcttggcc ttcggtgcgc ttcggatgag
      301 cggcgctcaa gtagacggcc gccgttcgcg agtggtcgcc cgcgctttcc tcgggcgaca
      361 gcgacgtcgc catgcggtga tccgtgccgc tgatgatcgt gatgcggtcc ttgaacgccg
      421 cgaccggctg caggatgcgc gacagctccc agctcttgcc ggagcccgtc ggccgccagt
      481 agccaggcgc cgcgccgtgc ggcacgtgca agaaactgta gcggctctgc gccgtcggcg
      541 tcgccgccat cgcgggcacc atcgcgtcga gcagcggcag agcgatcgtc gctccgattc
      601 cgcgcaagaa cgttcggcgg ggcatgtgtc gcttggttat gaacatcgct gctctctccg
      661 ttactcggtg tccgcgacga cttcagcgcc cgccggacct tcttccgcgg cgcgcatctg
      721 aaacgcggga ctctcgacga ttcctaggat gatgctcgaa aaacgatagt cgttcttggc
      781 cgcgccgcgc gcgatcgcgc gcacggtcgg catgtcgtag tactcgaggc cgcgcccgag
      841 cgcgtaggtc atgagcttct cggtcgtcgt ctgcacgaag cgatccgagt acttcaacag
      901 cgcattgcgc aggtcgacgg cgccgttgat ctgcgtgccg tcgacgagcc gacccgtcgc
      961 gtcgatcggc gtgccgtgct cctcagtgcg ccattggccg atcgcgtcga agttctcgag
     1021 tgcgaatccg accgggtcca tgatcttgtg gcaaccggcg cagaacggcc gctcgctgtg
     1081 caggcgcatc tgctcgcgta ccgttcgcgg atgggttttc gtgccggctt gttcctcgag
     1141 cgcgggcacg acgggcggcg gcgtcggcac aggggcgccg acgatgttct cgagcaccca
     1201 aacgccgcgc tggaccggcg acgtgcgctc cggcagcgag gtgacgagca ggatgctgcc
     1261 ctggcccagc aacccgcgcc gcgcttcgtt cgcgacgggc acgcggcgga actcgctgcc
     1321 gtagatgcct tccatgccgt agtggcgcgc gagacggtcg ttgacgaacg tgtaatccgc
     1381 ggtcagcagg tcgagcaagt tgcggtcttc gcgaacgatg ctcgcgaaca gcatctccgt
     1441 ctcggtgcgg aaggcctggc gcaggttgtc gtcgaagttc gggaactgct cgacggcgcc
     1501 gcccttcgtc tcgaggttgc gcaagtacag ccattggccg gcgaagttct cgatgaacgc
     1561 gttcgagcgc gtgtcgtcga gcatgcggcg gacctggcgg cgcagcgtcg ccggctcgtg
     1621 cagcttgtcg tcgatcgcga gcttgagcag ctcgtcgtcg gggatgctgc tccagaggaa
     1681 gaacgacaac cgcgacgcga gctcgagatc gctcaagcgg tgcaccgcgc cgggggcggc
     1741 atcgtcggca cgctcgaagc ggaacacgaa ctcgggtgaa gcgaggatgc gccgcagcgc
     1801 gagctcgatg ccggcttcga agtcgccttg ctcgcgaccg tcggcgtaaa agtccatcag
     1861 tgtcgacagt tgctcgcggt cgacaggatg gcggtacgcg cgcgtcgcga gcgtctcgac
     1921 gatctgcctc gcgcatggca tttcttcgcg cgccgtcgtc gggcggcaga cgaagatctt
     1981 ttgtcgggtc ggcgtgtcgc tgacgccggc gatatcgaac ggccccgtca cgctcatgct
     2041 cgcgaggtgc ggcacgaacg tccaatcggg atcgccgtcg acggccggct cgaacaggct
     2101 gaactcgaac ggctgaatga cgtcctcgac gggggcgtgg ttcttcttga tgaacgcggc
     2161 gccgatcgtg tgcggcccgg ccttgacggg catgcggatc gccgcgccgt cctcgacctt
     2221 gtagtcgctg agctcggcga gcagcacgcg ctgaccgtcg agcgtgacct cgagctgctc
     2281 ggtcttgccc gcgttcgcgc cgaacatgcg gccgccgcgc tcgacgaggt ccgcgcgaat
     2341 gacgtactcg gcgtcgacgg ggaactgatg cgtgaacgtg gcaccgccgc gcgtgccgaa
     2401 cggcatgccg ggcagatgct ggttctgcgt cagaccgccg ctgacgagat atttctcttg
     2461 ccgatccggc atcgtcgtgt cgccgatcgc aaggcggctg atcttgccgg cggccgcgac
     2521 gtaccgctcg agcagcacgg gcgatacgcc gagcgagccc gcgatgttgt cgaagccgaa
     2581 gctcgagtcg tcggccggca gcaacgccgt tgcatcgatt tcgagcgcga gcaagtcgcg
     2641 aacggcgttc gcgtactcgg tgcggttgac gcgatgcagg gcaggccggc ccgggttcgg
     2701 ctcggccgcg gccagccggt cgagctcggt ctcgagatag gtcaaaaact ccgccttctt
     2761 gttcgggtcg ggtcgttctt cagaggccgg cggcatcatg ccggcgccga gcttgcgtac
     2821 gaccttttcc caggtctcgc ggtcgtcggc gacg
//
LOCUS       DATLEE010000303         5279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9291402, whole genome shotgun sequence.
ACCESSION   DATLEE010000303 DATLEE010000000
VERSION     DATLEE010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9291402"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VM692_06405"
     CDS             complement(<1..579)
                     /locus_tag="VM692_06405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ51835.1"
                     /translation="MRRVKLCIVVPAHWEALMGGSQYQAKVLVDYLLEHYDVDIAYLT
                     TRADPSFEPKGYRIVPFSDRRGLRRYGSFFDVVRLYRALARERPDIILQFVGSAHTGI
                     AALYARRHGCKMIWRVTSDRTVEPETASWLQPHLKLERLFLNYGIRNASLILAQTESQ
                     RAAVAAAFPRAPVQVLPNFHPTAPACGRGGAGM"
     gene            complement(576..1400)
                     /locus_tag="VM692_06410"
     CDS             complement(576..1400)
                     /locus_tag="VM692_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HUQ51836.1"
                     /translation="MDSALRYQSPVVWNGRPIRSWAAFHSVVKQPDRPLLAKLDDYTA
                     PILVAGCQRSGTTALARLLKRADGVVDHEFGHDDELDGALLLAGHVTRPLEGRQCFQT
                     TYLNDRYPEYFEHRDFRLIWMLREPRSVVYSMLYNWKRGALNRLFDACGAEVLARGQH
                     EPPRFGATWLGPSRLTKACASYVAKTGQTFVLAERLGDRMAVVDYDELVAHKHELLPR
                     LCEFVGVPYSPRLADALHDRSVNKRSRLGKRDGETVDRFCLDVYDRARAVRTIGAA"
     gene            complement(1393..3246)
                     /locus_tag="VM692_06415"
     CDS             complement(1393..3246)
                     /locus_tag="VM692_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HUQ51837.1"
                     /translation="MVAIYGVLGDASRSELEAMGARLAHRGNFGAQWSPAPGVWLGMQ
                     SRQFADLATDGPLLFDGSLDNRWQLGTRRGAGTPPACDPSTDASMLLELLETEGPRAL
                     ELLAGSFAIAWWRAQDRTLLLARDRIGYGPLHFTVDRAGRFVFASEYKALLALETVDA
                     QPNRDAIQVLQNTKWTKPGETCLAGIYPVAPGTALEVDAGALSMQRYWNIPVAVKHDD
                     DERHAAELREVFLDTLQSQTRAYRRIGVSMSGGLDSAVVAAGARHVVGNKPLHTFTAG
                     YGPDDREIINAARVAETLKTEHHEIILRPEDLPKLLPEMVWHLEEPIGREDIAYLFVA
                     ARQAARHVDVILAGFGFDGLFAGLPRHRLVDLANKVPLARAPLEQFYDFTFRSVEPRT
                     PAGRALRYAYFRGSDYPAPRVLGARPLAKFTGFPRAMPQPLTSFLRRNLLLNPYQVAI
                     EHLYSAVGVRMNAQHTNPKFIAAAFSIPDRLKIRGTTQKYILRKACAGLLPAQTLGVG
                     KSFNRMKHDLELCESLDAMADTLLSRSDVAARGLFDPGYIAELRKRPAGQPYGRERIY
                     RLWTLLLTELWSREFLDGRGAPRPATVAAPRHDRVAQHAVSHLAPTGTAHG"
     gene            complement(3259..4275)
                     /locus_tag="VM692_06420"
     CDS             complement(3259..4275)
                     /locus_tag="VM692_06420"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUQ51838.1"
                     /translation="MKILVTGGTGFTGTALVRKLLADGHSVVALDYKEGLAASELKRR
                     GAEVLLGSVTDRELVTRAMQGVDVVHHLAAAFRELNVPEQHYYEVNVDGTRNVLAAAA
                     RAGVQKVIYCSTCGVHGNIDDPPASEDAPIQPADYYQQTKYEAEPFVREWNRRGLRTT
                     ILRPAAIYGPGDPERFFMIFKRVARGVFPMFGNGKTLYHPLYIDNLVDAFIAAMPLDV
                     GNGEAYLIADEHYVEIEELVKAVASALGVTVSIPHYPLLPLIAAGHICETLCKPLGVT
                     PPIFPRRVDWYRQNRAFDISKAKRELNYRPAVGLREGLMRTASWYRAEGYLPMPNVAP
                     ASAP"
     gene            complement(4307..>5279)
                     /locus_tag="VM692_06425"
     CDS             complement(4307..>5279)
                     /locus_tag="VM692_06425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ51839.1"
                     /translation="PIGFSVRKLLCDLALGWRLLTLTRQRRYDVLHAVEEAVFPSLIV
                     RGLGCRVVYDMDSSLPEQLLGKYRSLQRLEGLLRRFERLAIARSDLVLAVCEDLAARV
                     RAYATATPVDVLEDVSLLDDGAAGNVDDLRRDLPANGALVMYVGNLEHYQGVDLLLDA
                     VAALKAPPPFRLVAIGGNAAAVAAYRARAVRLGIDALVKFVGARPLKDLGAYLAQADV
                     LVSPRCSGQNTPMKLYSYLGAGKAVLATRIRSHTQVLTDDCALLVEPTAPALASGLET
                     LLRAPALREQLGSAARRLATTRYSLTQFRESVASAYRRLAIAPSGNA"
BASE COUNT          863 a         1724 c         1873 g          819 t
ORIGIN      
        1 catcccagcc ccgccgcggc cgcaggcggg cgccgtgggg tggaagttcg gcagcacctg
       61 cacgggcgcg cgcggaaacg ccgccgcgac ggccgcgcgc tgagactcgg tctgcgcgag
      121 gatcagcgac gcattgcgga tgccgtagtt caggaacagc cgctcgagct tcaggtgcgg
      181 ctgcagccac gaagcggttt ccggctcgac ggtccgatcg ctcgtgacgc gccagatcat
      241 cttgcagccg tggcggcggg cgtagagtgc cgcgatgccg gtatgagccg agccgacgaa
      301 ctgcaggatg atgtcgggac gctcgcgtgc cagcgcccga tagaggcgca cgacgtcgaa
      361 gaacgagccg tagcggcgca ggccccggcg gtccgagaac ggcacgatgc gatagccttt
      421 gggctcgaag ctcgggtcgg cgcgcgtcgt caggtacgcg atgtcgacgt cgtagtgctc
      481 gagcagatag tcgacgagca ctttggcctg atactgcgat ccgcccatca gcgcttccca
      541 atgggccggc acgacgatgc agagcttgac gcgcctcatg cagcaccgat ggttcgcacc
      601 gcgcgcgcgc gatcgtaaac gtcgaggcag aagcggtcga cggtctcgcc gtcgcgcttg
      661 ccgaggcggc tgcgcttgtt gacgctgcgg tcgtgcagcg catcggccaa gcgcggcgag
      721 tagggtacgc cgacgaactc gcaaagccgc ggtagcagct cgtgcttgtg ggccacgagc
      781 tcgtcgtagt cgacgacggc catgcggtcg ccgagtcgct cggccagcac gaacgtctgg
      841 cccgtcttcg ctacgtacga cgcgcaggct ttcgtgagcc gcgacgggcc gagccaggtg
      901 gcgccgaaac gcggcggctc gtgctgcccg cgggcgagca cctccgcgcc gcaggcgtcg
      961 aacaaccggt ttagcgcgcc gcgcttccag ttgtacagca tcgagtacac gacggagcgc
     1021 ggctcgcgca gcatccaaat tagcctgaag tcgcggtgct cgaagtactc gggatagcgg
     1081 tcgttcaagt acgtcgtttg gaagcactgg cggccctcga gagggcgcgt cacgtgcccc
     1141 gcgagcagca gcgcgccgtc gagctcgtcg tcgtgaccga actcgtgatc gacgacgccg
     1201 tcggcgcgct tcagcaagcg cgcgagcgcc gtcgtgccgg aacgctggca gccggcgacg
     1261 agaatcggcg ccgtgtagtc gtcgagcttc gcgagcaacg gtcgatcggg ctgtttgacg
     1321 acggaatgaa acgcggccca cgagcgaatc ggtcggccat tccagacgac gggggattga
     1381 taacggaggg cgctatccat gtgctgttcc tgtcggagcg agatggctga cggcgtgctg
     1441 cgcgacgcgg tcgtgacgcg gcgccgcgac ggtcgccggc cgcggcgcgc cgcggccgtc
     1501 caagaattcg cggctccaga gctccgtgag cagcagcgtc cacaggcggt agatgcgctc
     1561 gcggccgtaa ggctggccgg cgggacgctt gcgcaattcc gcgatgtaac ccgggtcgaa
     1621 cagcccgcgc gccgcgacgt cgctgcgcga gagcagcgta tcggccatcg cgtcgagcga
     1681 ctcgcacagc tcgaggtcgt gcttcatgcg gttgaagctc ttgccgacgc cgagcgtttg
     1741 cgccggcagg agccccgcgc acgccttgcg caaaatgtac ttctgcgtcg tgccgcggat
     1801 cttgagccgg tcgggtatcg agaacgccgc cgcgatgaac ttcggattcg tgtgctgtgc
     1861 gttcatgcgc acgcccacgg ccgagtagag gtgctcgatc gcgacttgat aagggttcag
     1921 cagcaagttg cgccgcaaga acgacgtcag cggttgcggc atcgcccgcg ggaagcccgt
     1981 gaacttcgcc aacggccgcg cgccgagcac gcgcggcgcc ggataatcgc tgccgcggaa
     2041 atacgcgtag cgcagcgcgc ggccggccgg cgtgcgcggc tcgacgctgc ggaaagtgaa
     2101 atcgtagaac tgctcgagcg gcgcgcgcgc gagcgggacc ttgttcgcga gatcgacgag
     2161 ccggtgccgc ggcaggcccg caaacaggcc gtcgaagccg aagccggcga gaatcacgtc
     2221 gacgtggcgc gcggcttggc gcgccgcaac gaatagatag gcgatgtctt cgcggccgat
     2281 cggctcctcg agatgccaga ccatttcggg caggagcttc ggcaggtcct cgggccgcaa
     2341 gatgatctcg tggtgctccg tcttcagcgt ctcggcaacg cgcgcagcgt tgatgatctc
     2401 gcggtcgtcg gggccatacc cggccgtgaa cgtgtgcagc ggcttgttgc cgacgacgtg
     2461 ccgggccccg gccgcgacga cggcggagtc gagcccgccg ctcatcgaga cgccgatgcg
     2521 gcggtatgcg cgcgtctgcg actgcagcgt gtcgagaaac acctcacgca gctctgcggc
     2581 gtgccgttcg tcgtcgtcgt gcttcacggc gacagggatg ttccagtagc gctgcatcga
     2641 cagtgcgccg gcatcgacct cgagagccgt gccgggcgcg acgggataga tgccggcgag
     2701 gcacgtctcg ccgggcttcg tccatttcgt gttctgcagc acttgaatcg cgtcgcggtt
     2761 cggctgcgcg tcgacggtct cgagcgcgag cagcgctttg tactcgctcg cgaagacgaa
     2821 gcgcccggca cgatcgacgg tgaagtgcaa cgggccgtaa ccgatgcgat cgcgagccaa
     2881 gagtaaggtg cggtcctgtg cgcgccacca agcgatcgcg aacgatcccg ccagcagctc
     2941 gagcgcgcgc gggccctcgg tctcgagcag ctcgagcagc atgctcgcgt cggtgctcgg
     3001 gtcgcaggcg ggcggcgtcc ccgcgccgcg gcgcgtcccg agctgccagc ggttgtcgag
     3061 cgagccgtcg aacagcagtg ggccgtccgt tgcgaggtcg gcgaactggc ggctctgcat
     3121 tccgagccac acgcccggcg ccggcgacca ctgcgcgccg aagttgccgc ggtgcgcgag
     3181 ccgtgcgccc attgcttcga gctccgagcg gcttgcgtcg ccgagcaccc catagatcgc
     3241 taccatcgat attgacctct aaggcgcact ggccggcgca acgttcggca tcggcagata
     3301 accctcggcg cggtaccagc tcgccgtgcg catcaggcct tcgcgcagcc ccaccgccgg
     3361 gcggtaattg agctcgcgct tggccttgct gatgtcgaaa gcacggttct gtcggtacca
     3421 atcgacgcgc cgcgggaaaa tcggcggcgt cacgccgagc ggcttgcaga gcgtctcgca
     3481 gatatggccg gctgcgatca acggcagcag cgggtagtgc gggatgctga ccgtaacccc
     3541 gagcgccgag gcgacggctt tgacgagctc ttcgatctcg acgtagtgct cgtcggcgat
     3601 caagtaagcc tcgccgttgc cgacgtcgag cggcatggcc gcgatgaagg cgtcgacgag
     3661 gttgtcgatg tagagcgggt gatacagcgt cttaccgttg ccgaacatcg ggaacacgcc
     3721 gcgcgccacg cgcttgaaga tcatgaagaa ccgttcggga tcgccgggcc cgtagatcgc
     3781 agccgggcgc aggatcgtcg tgcgcagccc gcggcggttc cactcgcgca cgaacggctc
     3841 ggcctcgtac ttcgtttgct ggtagtagtc ggccggttgg atcggggcgt cctcgctggc
     3901 cggcggatcg tcgatgttgc cgtgcacgcc gcaggtgctg cagtagatga ccttctgcac
     3961 gccggcgcgc gcggcggccg ccagcacgtt gcgcgtgccg tcgacgttga cctcgtagta
     4021 gtgctgctcc ggcacgttga gctcgcggaa cgccgcggcc aagtggtgca cgacatcgac
     4081 accctgcatt gcgcgcgtga cgagctcgcg gtccgtcacg gaccccagca gcacttcggc
     4141 gccgcggcgt ttgagctcgc tcgcggcgag gccttccttg tagtcgagcg ccacgacgga
     4201 atgaccgtcg gccagcagct tgcggaccag agccgtcccg gtgaagccgg ttccgcccgt
     4261 gaccaaaatt ttcatcgttg cacctcgtga ttcgaaaggg tggtcgtcat gcgttgcccg
     4321 aaggcgcgat tgcgagacgc cgataggcgc tcgcgaccga ttcacggaac tgagtgagcg
     4381 agtagcgcgt cgtcgcgaga cggcgcgcgg ccgagccgag ctgctcgcgc agcgcaggcg
     4441 cgcgcagcag cgtttcgagc ccgctcgcga gggccggcgc cgtcggctcg acgagcagcg
     4501 cgcaatcgtc ggtcaacacc tgcgtgtgcg agcggatgcg cgtcgcgagc acggccttgc
     4561 cggcgccaag atacgagtac agcttcatcg gcgtgttctg gccgctgcag cgcggcgaca
     4621 cgagcacgtc ggcttgggcg agataggcgc cgaggtcctt cagcggccga gcgccgacaa
     4681 acttcaccag cgcatcgatg ccgagccgga cggcgcgcgc gcgataggca gcgaccgcgg
     4741 ccgcgttgcc gccgatcgcg acgagcctga acggcggcgg agccttcagc gcggccaccg
     4801 cgtctagcag taagtcgacg ccttggtaat gctcgaggtt gccgacgtac atgacgagcg
     4861 cgccgttggc gggcagatcg cggcgcaagt cgtcgacgtt gcctgccgcg ccgtcgtcga
     4921 gcagcgagac gtcctcgagg acgtcgacgg gcgtcgccgt cgcgtacgct cgcacgcgtg
     4981 cggcgagatc ctcgcagacg gcgagcacga gatcggagcg tgcgatcgcg agccgctcga
     5041 agcgccgcag caagccttcg agccgctgca gcgagcgata cttgccgagt aattgctcgg
     5101 gcaacgacga atccatgtcg tagacgacgc ggcagccgag gccgcgcacg atgaggctcg
     5161 gaaatacggc ttcctcgacg gcatgcagca cgtcgtagcg ccgttggcgc gtcagcgtca
     5221 gcagccgcca tccgagcgcg aggtcgcaga gcagcttacg cactgagaag ccgatcggc
//
LOCUS       DATLEE010000304         9872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4821694, whole genome shotgun sequence.
ACCESSION   DATLEE010000304 DATLEE010000000
VERSION     DATLEE010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4821694"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1959)
                     /locus_tag="VM692_06430"
     CDS             complement(<1..1959)
                     /locus_tag="VM692_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUQ51840.1"
                     /translation="MRCTLVVAALAGSFFAAGLVAQPVHMQEHNPALRAPRAAGAADE
                     APGIIVKFRSAAARGDAQLQAAADPVSKLAKRGVAIRESRRLPAGLHVLRAERLPGES
                     PAEQLARVRADADVEFAEPDARRYPHALPNDPLYTGQWYLQNRSDAPSAIHAEAAWDA
                     GVGNKGVVIAVLDTGVLFDHPDLKRAHLGGRLLPGYDFISNAAAANDGNGRDANPSDA
                     GDWVTQPEANQGQFAGCDVSNSSWHGTRVAGIIGALTNNGEGVAGGSWSPWILPVRVL
                     GKCGGLDSDIIEAMAWAGGIHVDGVPDNPYPAKIENMSLGGYGPCGAAYASVITELAA
                     RGVLVVASAGNEGGPVGTPASCAQAAAVTGLRHVGTKVGFASLGPQIAVAAPGGNCVN
                     TNGGPCLFSIDTTYNTGTQAPAAHGYTNQTNVNVGTSFSAPIVSGIVGLMAGANGNLG
                     AAQLRARLREGATAPFPRSTDPTIPMCHVPTGPNDLQASECNCTTSTCGAGMANAEGA
                     LAAALRPIAAVSAPARVAAGQKVSLTGAGSAGACHRTIASYAWEVVAGSGVLTGPSNT
                     ATTSVDAPSSGSFTVRLTVTDDLGKQDAADIVVASTTTATSAPAAAGDNACLADIDPP
                     PAIRIAVSPLSVAVEAGSGAQLFTAAVTN"
     gene            complement(1962..2306)
                     /locus_tag="VM692_06435"
     CDS             complement(1962..2306)
                     /locus_tag="VM692_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51841.1"
                     /translation="MRIGIARVLPRFMLASLICATAACAQPRGDASEPKGCSLQAIVA
                     LQGDPVPALVADLGRASGARLELVRTMTTNLHLFSVTAPGPQADCLAAVERLRADARV
                     RSVDVDQRRQAQ"
     gene            complement(2399..3316)
                     /locus_tag="VM692_06440"
     CDS             complement(2399..3316)
                     /locus_tag="VM692_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ51842.1"
                     /translation="MSLVRNWSLGVTLVGMAAPAAAQLNDLDEALRTTPITTQQLGDN
                     LHVLFGVGGNILVSIGASGTLIVDDQFPLMVPKYKAKIGELGGGAVNFAINTHWHFDH
                     ADGNQVLGPEGTWLVSHEIARQMMMKNNVINLVTQKRDQPAYAETAWPVLTYDETMRF
                     HFNGERIDLVHYGPAHTTGDSAVIFREHKVVHMGDVYNNAGYPFIDADNGGSLSGVIA
                     FTSKVLEQIDSTYTVVPGHGPVADAQALADYVAMLTMIRDRMSALIGSGATFEQVVAA
                     RPTSEWDARKGDPASFLDRAYSSLTRERR"
     gene            complement(3361..3888)
                     /locus_tag="VM692_06445"
     CDS             complement(3361..3888)
                     /locus_tag="VM692_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51843.1"
                     /translation="MRSLIKRVLIVLVLSLVATYVALSEGPAGLQLVVATWIFVVPLW
                     LAFFPFNGATTFGVGVAAYPLLLGSLPPLPGLTPDAAATYGAVGLAAIACVLTFRLER
                     VLGRQVWYRIPRHVLRLAVFTGIAYAALLRYEGHLLTDPMSVHLAALRANPVGTAAVV
                     LGTLAVLHLLLRWRH"
     gene            complement(4105..5133)
                     /locus_tag="VM692_06450"
     CDS             complement(4105..5133)
                     /locus_tag="VM692_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HUQ51844.1"
                     /translation="MIKAGIAGLGWWGKTLVEGVADSKDIQFVAGSARNHTDDIKSFA
                     KTQKFELYKSFEDMLKHPGLDAVVLATPHSMHVPQTIAAAKAGKHVFCEKPFALHGAE
                     AREAVDAVEKAGVTLGLGYNRRWHPEMTKLRNMIDSGELGVILHVEATMTFPNALLLK
                     ADAWRASREETPCGGLTPMGVHAVDAMIDLCGPIDEVYCQSFRRAVSIDADDTTSILF
                     RMKEGMSGYLGTMTATGGGFDFVVYGSKGFVRFEGMTHVAGAPSEERRTRLFGTCTFK
                     PAQGPAKTWEAERLDLTKVTLEAFARAAEGKEKFLIPVEEMVHGAAVTEAIVRSSETH
                     VPEKVRKA"
     gene            complement(5146..6138)
                     /locus_tag="VM692_06455"
     CDS             complement(5146..6138)
                     /locus_tag="VM692_06455"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ51845.1"
                     /translation="MGCAAGRDEARHDRRLRAARARLGQRPARLTRLKLGRTRVKVGF
                     VGLGRMGQAMAARLLGGGHELVVYNRSREKAAELEKGGAKVAGSIAEACKGREVVISM
                     VADDKALTEVVKEISGALEKGAIHVAMGTHGVHVLREVDGVHGKLGQQFVASPVLGRP
                     EAAAAGQLNIVPAGPAAAIEKLAPLNALMGRRTFPAGTNPEGAAAVKLANNMLIGCAI
                     EAMGEAFSLVRKYGVDPAAFYEVFTDGLFAAPAYKVYGNIIVKQLYGQAGFTTTLGLK
                     DTGLMLAAGLAENVPLPSLNVYRDKLLAAMANGDANSDWSVIAKHQARDAGLEK"
     gene            complement(6048..7250)
                     /gene="eboE"
                     /locus_tag="VM692_06460"
     CDS             complement(6048..7250)
                     /gene="eboE"
                     /locus_tag="VM692_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HUQ51846.1"
                     /translation="MDLGNGLGHLTYSTLVHPGDTWAEMWDSLNRYVPQVKARVSPKQ
                     SFGVSLRLSASSAQTLTGDREQRERLKDFLKQNDMYLYTVNAFPYGPFKNRIVKKDVY
                     EPDWSTNEREIYTSQVADILADVASPNVNPSIQSPPLGFKPKVTGPAYVNEFTRHVIN
                     TVAHMVKLEKRTGRTVTLCLEPEPACFLETTDEAVDYFTNHLYASEAVRMLVTATGLN
                     KSQAETALRKHLGMVYDICHQAVEYEDIGASLRKLADNGIPVFKLQEAAALRVPNVTK
                     ETVDAMRPFAETVYLTQTIEKIDGKLNRYLNLEDAFAAFEANPNLKREWRTHVHVPVF
                     LDKIGQHFETTRFAIEDALKFHKKNKLSPQLEIETYTWDVLPDEMKHGTIVDYVQREL
                     DWVKGQLV"
     gene            7448..8710
                     /locus_tag="VM692_06465"
     CDS             7448..8710
                     /locus_tag="VM692_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HUQ51847.1"
                     /translation="MLRATANQLLPTTITGSLPRPSWYTENLGLKSFLQAMVETRFRE
                     QYEDTLAVYMGEQAIAGLDIVTDGDVRFTQDIGGQSWTSYPPFHMAGFDGVPKMAPIG
                     GGGIGFPRGHILHDYIEARLMPSIVGPIGRGQMQYAQIWKAAQRTTDKPVKFGTVTPE
                     LVAFAVQDFYYKDVRERIWAISDAFNEELHALADAGCPVIQMEEPQIHLLAARGVVDK
                     IINPEFMLEVFNNTVRGLRAKTEVWCHTCWGNPSQQRMFAKVQSYKPALKILNKVDAD
                     VITFESVSSGGQDLEAIGQEITDMKVAIGVIDHHTLQVESATEVADHIRLALKHIPAE
                     RLVLSSDCGMGREGMARRHALYKMISLVKGTNIVRKELGIKEAECIAADANLTLLDVK
                     RAAPEAAAAPAKKSGKPATAKNKKKGKK"
     gene            complement(8780..9082)
                     /locus_tag="VM692_06470"
     CDS             complement(8780..9082)
                     /locus_tag="VM692_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008388532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HUQ51848.1"
                     /translation="MAKGYWIARVDVADPERYKTYIAANAVPFKKFGARFVVRAGRFE
                     NPEGSSRPRNIVIEFPSYEVALECYKSREYQEAIKHRLAVATIDLVIVEGYDGPQP"
     gene            complement(9088..>9872)
                     /locus_tag="VM692_06475"
     CDS             complement(9088..>9872)
                     /locus_tag="VM692_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HUQ51849.1"
                     /translation="TRRDVVGIGAAAGVAALAAGQGAAQSRTGRRVTIDAKPQPITID
                     TATTAVIVVDMQNDFGSKGGMFERAGIDISGIRKAVPPTARVLDAARKSGIKVVYLKM
                     AYQPDLSDLGHEDSVNRVRHLALGVGQHVTAPDGRPSRVLVRDTWNSEIVDELKPHAD
                     DIVLYKTRFSGFYKTDLDERLRELGAKHLIITGCTTSICVESTVRDAMFRDYLCILMA
                     DCMDEPIGNTFARTNHDASLLSTEVLLGWVSDSSELSKSLHA"
BASE COUNT         1700 a         3256 c         3148 g         1768 t
ORIGIN      
        1 gttcgttacc gctgccgtga acagctgcgc accgctgccg gcctcgacag caaccgacag
       61 cggcgacacg gcgatcctga tcgcgggcgg cgggtcgatg tccgctaggc aggcgttgtc
      121 ccccgcagcg gccggcgccg acgtggcggt cgtcgtcgac gcgacgacga tgtcggccgc
      181 gtcctgcttg ccgaggtcgt cggtcacggt caagcgcacc gtgaaggacc cgctcgaggg
      241 cgcgtcgacc gacgtcgtcg ccgtattgct cggcccggtc aatacgccgg acccagccac
      301 gacttcccaa gcataggaag cgatggtgcg atggcaggcg cccgcgctgc cggctccggt
      361 taggctcacc ttttgtccgg ccgcgaccct cgccggcgcc gacacggcgg cgatcggccg
      421 cagcgccgct gcgagcgcgc cctccgcgtt cgccatgccg gcgccgcacg tgctcgtcgt
      481 gcagttgcat tcgctggcct gcagatcgtt cggtcccgtc ggcacgtggc acatgggaat
      541 cgtcggatcc gtagaccgcg ggaacggcgc cgtcgcgcct tcgcgcaagc gcgcacgtag
      601 ttgtgcggca ccgaggttgc cgttcgcacc ggccatcagc cctacgatcc cggagacgat
      661 cggcgccgag aagctcgtgc cgacgttgac gttggtctga ttcgtgtagc catgcgccgc
      721 aggcgcctga gtgccggtgt tgtatgtcgt gtcgatcgag aacaggcacg ggccgccgtt
      781 cgtgttgacg cagttgccgc ccggcgcggc gacggcgatc tgcgggccca agctcgcgaa
      841 gccgaccttc gtgccgacgt gccgcaagcc cgtgacggcc gcggcttgcg cacagctcgc
      901 cggcgttccg acgggcccgc cttcgttgcc ggccgatgcg acgacgagca cgccgcgcgc
      961 cgccagctcg gtaatcacgc tcgcgtaggc ggcgccgcac ggtccataac cgccgaggct
     1021 catgttctcg atcttcgcgg ggtacggatt gtcgggcacg ccgtcgacgt gaatgccgcc
     1081 tgcccacgcc atcgcctcga tgatgtccga gtcgaggccg ccgcatttgc cgagcacacg
     1141 gaccggcaag atccatgggc tccaactgcc tcccgccacg ccctcgccgt tgttcgtcag
     1201 cgccccgatg attccggcga cgcgcgtgcc gtgccacgag ctgtttgaaa cgtcgcaacc
     1261 tgcgaactga ccttggttcg cttccggctg cgtgacccag tcgccggcgt cggagggatt
     1321 tgcgtctcgg ccgttgccgt cgttggcggc agcggcattc gagatgaagt cgtagcccgg
     1381 tagcaaacgc ccgccgagat gcgcgcgttt gagatcgggg tgatcgaaca gcacgccggt
     1441 atcgagaacc gcgatgacca cgcctttgtt gccgacgccc gcgtcccaag cggcctcggc
     1501 gtgaatcgcg ctcggcgcgt cgctgcggtt ctgtagatac cactgacccg tgtacagcgg
     1561 atcgttcggc agcgcgtgcg gatagcgccg cgcgtccggt tccgcaaact cgacgtcggc
     1621 atcggcgcgc acacgtgcga gctgctcggc cggcgattcg ccgggcagcc gttcggctcg
     1681 cagcacatgc aaaccggccg gcagcctgcg tgactcccgg atggcaacgc ctcgcttcgc
     1741 cagcttgctg acggggtccg ctgcggcttg caactgcgcg tcgcctcgcg ccgcggccga
     1801 gcgaaatttc acgatgatgc cgggcgcttc atcagcagcg ccggccgctc gcggagcgcg
     1861 cagcgccggg ttgtgctcct gcatgtggac cggctgcgcg acgaggcctg cggcgaagaa
     1921 cgagcctgcg agggccgcga caaccagagt gcagcgcatg ttcactgagc ctgccgccgc
     1981 tgatcgacgt ccacggaccg cacgcgcgca tcggcacgca agcgctcgac cgcggccaag
     2041 cagtcggcct gaggtcccgg tgccgtgacc gaaaacaaat gcagattggt cgtcattgtg
     2101 cgcacgagct cgagccgcgc gccgctcgcc cggccgaggt cggcgacaag cgccggcacc
     2161 ggatcgcctt gcagcgcaac gatcgcctgc agcgagcacc ccttgggctc gctcgcgtcg
     2221 ccgcgcggct gcgcgcacgc cgcggttgca cagattagcg acgccagcat gaagcggggc
     2281 aacactcgcg cgataccgat tctcatgatg cgactctcgt ccgctttcgc tgaacgcacg
     2341 atgaacgcaa agcagcgcaa acagtcggca ccaagcacct gcccacgaga accgattgtc
     2401 agcggcgctc gcgcgtgagg ctcgaatagg cgcggtcgag gaagcttgca gggtcgccct
     2461 tgcgcgcgtc ccactccgac gtcgggcgag ccgcgacgac ttgctcgaac gtggcaccgc
     2521 tgccgatcaa ggcactcatg cgatcgcgaa tcatcgtcag catcgcgacg tagtcggcga
     2581 gcgcctgcgc gtcggcgacg gggccgtgac ccggcacgac ggtatacgtc gagtcgatct
     2641 gctcgagcac tttcgaagtg aacgcgatga cgccgctcag agagccgccg ttgtcggcgt
     2701 cgatgaacgg atagccggcg ttgttgtaga cgtcgcccat gtgcacgact ttgtgctcgc
     2761 gaaaaatcac ggccgagtcg cccgtcgtgt gcgcggggcc gtaatgcacg agatcgatgc
     2821 gctcgccgtt gaagtgaaag cgcatcgttt cgtcgtaggt cagcaccggc catgccgtct
     2881 cggcatacgc cggctgatcg cgcttctgcg tcacgaggtt gatgacgttg ttcttcatca
     2941 tcatttgccg agcaatttcg tgcgagacga gccacgtgcc ctcgggaccg agcacctgat
     3001 tgccgtcggc atggtcgaag tgccagtgcg tgttgatcgc gaagttgacg gcgccgcctc
     3061 cgagctcgcc gatcttcgct ttgtacttcg gcaccatcag cgggaactga tcgtcgacaa
     3121 tcagcgtgcc gctcgcgccg atcgagacga ggatgttgcc gccgacgccg aacagcacat
     3181 gcaagttgtc gccgagctgc tgcgtcgtga tcggcgtcgt gcgcaacgcc tcgtcgaggt
     3241 cgttcagttg tgcggccgcg ggcgccgcca ttccgacaag agtcacaccg agcgaccaat
     3301 tgcgaacaag actcatcgaa gccctccttg gagccgaact agcgtcgtaa cggtgtgtgc
     3361 tcagtgccgc cagcgcagca agaggtgcag gacggcgagt gtgccgagga cgacggcggc
     3421 cgtcccgacg gggttcgcgc gcaacgcggc gaggtgcacc gacatgggat cggtcagcaa
     3481 gtgaccttcg taacgtagca gcgccgcgta cgcgatgccc gtgaacacgg cgagccgcag
     3541 aacatggcgc gggatgcgat accacacctg gcgcccgagc accctttcca gccgaaacgt
     3601 aagcacgcac gcaatcgcgg cgaggccgac cgcgccgtag gtggcggccg catccggcgt
     3661 cagccccggc agcggcggca agctaccgag cagcagcgga tacgccgcga caccgacgcc
     3721 aaacgtcgta gcgccattga acggaaaaaa cgcaagccag aggggcacga cgaagatcca
     3781 ggtcgccacg acgagctgca aacccgccgg cccttcgctg agcgcgacgt aggtagcgac
     3841 aagactcaag acgagcacga tcagcacgcg cttgatcagc gaccgcatgg tcgcgaagcc
     3901 tagcgagccg cccgcagcgc cgccggcagc cgaagtgcct gaaatcgtgg gattccaagc
     3961 gcgggcgctc gacccggacg ggtaatcgtc gttgctcacg cggtctcgct tgtcgttatc
     4021 gtcgttgcgg ccgctccgat cctacgccgc tcgcggcgca tttagaacga ggcgttcgcc
     4081 tagcggaagg cgagcggcgg gctgctaggc cttgcgcact ttctccggga cgtgcgtctc
     4141 ggaagagcga acgatcgctt cggtgacggc ggcgccgtgc accatttcct cgacggggat
     4201 caggaatttt tctttgccct cggcggcacg cgcgaacgcc tcgagcgtga ccttcgtgag
     4261 gtcgagccgc tcggcctccc acgtcttcgc cgggccttgc gccggcttga aggtgcaggt
     4321 gccgaacaga cgcgtgcggc gctcttcgga cggcgcgccg gcgacgtgcg tcatgccttc
     4381 gaagcgcacg aagcccttcg agccgtaaac cacgaagtcg aagccgccgc ccgtcgcggt
     4441 catcgtgccg aggtagcccg acatgccttc tttcatgcgg aacaggatcg acgtcgtatc
     4501 gtcggcgtcg atggacaccg cgcgccggaa gctctggcag tagacctcat cgatggggcc
     4561 gcagaggtcg atcatggcgt cgacggcgtg cacgcccatc ggcgtcaaac cgccgcacgg
     4621 cgtctcttct ctgctcgcgc gccacgcgtc ggccttgagc agcagcgcgt tcgggaacgt
     4681 catcgtcgcc tcgacgtgca gaatcacgcc gagctcgccg ctgtcgatca tgttacgcag
     4741 cttcgtcatc tcgggatgcc agcgccggtt gtaacccaag cccagcgtga cgccggcctt
     4801 ctcgacggcg tcgacggctt cgcgagcctc ggcgccatgc agtgcgaatg gtttctcgca
     4861 gaacacatgc ttgccggcct tagccgccgc tatggtttgc ggcacgtgca tcgaatgcgg
     4921 cgttgcaagc acgacggcat cgaggcccgg atgcttcagc atgtcctcga agctcttgta
     4981 gagctcgaac ttctgcgttt tcgcgaacga cttgatgtcg tccgtatggt tacgcgcgga
     5041 gcccgcgaca aattgaatgt ccttcgagtc ggcgacaccc tcgacgagcg tcttgcccca
     5101 ccaaccgagc cccgcgatgc ctgctttgat cactgtcgaa actccctatt tctcgaggcc
     5161 ggcgtcgcgc gcctggtgct tggcgatcac tgaccagtcg ctgttcgcgt cgccgtttgc
     5221 catcgccgcg agcagcttgt cgcgatagac gttcaagctc ggcagcggca cgttctcggc
     5281 gagacccgcc gcgagcatga gccccgtgtc cttgaggccg agcgtcgtcg tgaacccggc
     5341 ttggccgtac aactgcttga cgatgatgtt gccgtagacc ttgtacgccg gcgccgcaaa
     5401 cagtccgtcc gtgaagactt cgtagaatgc cgccggatcg acgccgtact tgcgcacgag
     5461 cgagaacgcc tcgcccatcg cttcgatggc gcagccgatc agcatgttgt tcgcgagctt
     5521 cacggcggcc gcgccttcgg gattcgtgcc ggccgggaac gtgcggcggc ccatcagcgc
     5581 gttcaaaggc gccagcttct cgatggccgc agcgggacct gccggcacga tgttgagctg
     5641 acccgcggct gccgcctcgg ggcggcccag cacgggcgac gcgacgaact gctgaccgag
     5701 cttgccgtgg acgccgtcga cttcgcgcag cacgtgcacg ccgtgcgtgc ccatcgcgac
     5761 gtggatcgcg cccttttcga gcgcgcccga gatctccttg acgacctcag tcagtgcctt
     5821 gtcgtcggcg accatgctga tgacgacctc gcggcctttg caggcttccg cgatcgagcc
     5881 ggcgacttta gcgccgccct tctcgagctc cgcggctttc tcgcgcgagc ggttgtaaac
     5941 gacgagctcg tgaccgccgc ccaagaggcg cgcagccatt gcctgaccca ttcggccgag
     6001 gccgacgaaa ccgactttca ctcttgttct cccaagcttg agacgcgtta aacgagctgg
     6061 cctttgaccc aatcgagctc gcgctgcacg tagtcgacga tcgtgccgtg cttcatctcg
     6121 tccggcagca catcccacgt gtaggtctcg atctcgagct gcggcgacag cttgttcttc
     6181 ttgtggaact tcagcgcgtc ttcgatcgcg aaacgcgtcg tctcgaagtg ctggccgatc
     6241 ttgtcgagga acaccggcac gtgaacgtgc gtgcgccact cgcgtttcaa gttcgggttg
     6301 gcctcgaacg cggcgaacgc gtcctcgagg ttcaagtagc ggttcaactt accgtcgatc
     6361 ttctcgatcg tctgcgtgag gtacacggtc tccgcgaacg gccgcatcgc gtcgacggtt
     6421 tccttcgtga cgttcggcac gcgcagcgcc gcggcttcct gcagcttgaa cacggggatg
     6481 ccgttgtcgg cgagcttgcg caggctcgcg ccgatgtctt cgtactcgac ggcttggtgg
     6541 cagatgtcgt agaccatgcc gaggtgcttg cgcagcgcgg tttcagcctg cgacttgttc
     6601 aggcccgtcg ccgtcacgag catgcgaacg gcttcgctcg cgtacagatg attcgtgaag
     6661 taatcgacgg cttcgtcggt cgtctcgagg aaacatgcgg gctcgggctc gaggcacagc
     6721 gtgacggtgc gacccgtgcg cttctcgagc ttgaccatgt gcgcaaccgt gttgatgacg
     6781 tggcgcgtga actcgttgac gtacgcggga cccgtgacct tcggcttgaa gccgagcggc
     6841 gggctctgga tcgacggatt gacgttcggc gaagcgacgt cggcgaggat gtccgctacc
     6901 tgcgacgtgt agatctcgcg ctcgttcgtg ctccagtccg gctcgtacac gtccttcttt
     6961 acgatgcggt tcttgaacgg cccgtacggg aatgcgttga ccgtatacag gtacatgtcg
     7021 ttctgtttca ggaagtcctt gagccgctcg cgctgctcgc ggtcgcccgt cagcgtttgc
     7081 gccgacgacg ccgacaggcg cagggaaacg ccgaaagact gcttcggcga gacacgtgct
     7141 ttcacctgcg gaacgtaccg gttcaggcta tcccacatct cggcccaggt gtcgcccggg
     7201 tgaaccagcg tcgaataggt cagatgaccc aggccattgc ccaaatccat gtcgtgctcc
     7261 tcgttaactt tgcgattccg ctcagcggcg ttcgtcaatt ccgctgccgc tttcgtcgag
     7321 ctgatcgttc cgattgctcg atccgtatag ggttgtgggg aaacgattaa ttcaatattg
     7381 tatccgccct tgctaaaaat gtcggctccc agccggcaac cgaataacac ctaacggggt
     7441 attacgaatg ctacgtgcga cggcaaacca actgctgcct acaaccatta cgggctcctt
     7501 gccgcgcccg agctggtaca ccgagaactt ggggctcaag tcgttcctcc aggcgatggt
     7561 cgagacccgc ttccgcgagc aatacgagga cacgctcgcc gtctacatgg gcgagcaggc
     7621 gatcgccggc ctcgacatcg tgaccgatgg tgacgtgcgt ttcacgcaag acatcggcgg
     7681 ccagagttgg acgagctatc ccccgtttca catggcgggc ttcgacggcg tgccgaagat
     7741 ggccccgatc ggcggcggcg gcatcggttt cccgcgcggc cacattcttc acgactacat
     7801 cgaagcgcgt ctgatgccga gcatcgtcgg ccccatcggc cgcggtcaga tgcagtacgc
     7861 gcagatctgg aaagccgcgc agcgcacgac cgacaagccc gtgaagttcg gcaccgtgac
     7921 gcccgagctc gtggcgttcg ccgtgcaaga cttctactac aaggacgttc gcgagcgcat
     7981 ctgggcgatc agcgacgcgt tcaacgaaga gctgcacgcg ctcgccgacg ccggctgccc
     8041 cgtcattcaa atggaagagc cgcaaatcca cttgctcgcg gcgcgcggcg tcgtcgacaa
     8101 gatcatcaac cccgagttca tgctcgaggt gttcaacaac accgtgcgtg gcttgcgagc
     8161 gaagaccgag gtctggtgcc acacctgctg gggcaatccg tcgcagcagc gcatgttcgc
     8221 gaaagtgcag agctacaagc cggcactgaa gatcctgaac aaggtcgacg cggacgtcat
     8281 cacgttcgag tcggtcagct cgggcggtca ggatctcgaa gcgatcggcc aagagatcac
     8341 cgatatgaag gtcgcgatcg gcgtcatcga tcatcacacg ctgcaggtcg agagcgcgac
     8401 cgaagtcgcg gaccacatcc gcttggcgtt gaagcacatc cccgccgagc gcttagtgct
     8461 gtcgtccgac tgcggcatgg gccgcgaagg catggcgcgt cggcacgcgc tctacaagat
     8521 gatctcgctc gtcaagggca cgaacatcgt ccgcaaagag ctcggcatca aagaagccga
     8581 gtgcatcgcc gcggatgcga acctcacgct gctcgacgtc aagcgcgctg cgcccgaagc
     8641 ggccgcggcg ccggcgaaaa agagcggcaa gcccgcgacg gcgaaaaaca agaagaaggg
     8701 caagaagtaa ttctttgcaa cagcccgcca ccgcctcgag cggtggcggg cttttttctt
     8761 gccgcaatca ctcctgctct cagggctgcg gcccgtcgta gccttccacg atcacgagat
     8821 cgatcgtagc aacggccaga cggtgcttga tcgcttcctg atattcgcgc gacttgtagc
     8881 actcgagcgc gacttcgtag gacggaaact cgatcacgat attgcgcggg cgactcgacc
     8941 cttccggatt ctcgaaacga ccggcccgga ccacgaaccg cgcgccaaac ttcttgaacg
     9001 gcacggcatt cgcagcgatg tacgttttgt accgctccgg gtccgcgaca tcgacacgtg
     9061 cgatccagta gcccttcgcc acgttcgcta cgcgtgcagc gacttcgaga gctcgctcga
     9121 gtccgacacc cagccgagca gcacttcggt cgataacagc gatgcatcgt ggttcgtgcg
     9181 cgcgaacgta ttgccgatcg gctcgtccat gcagtccgcc atgaggatgc agaggtagtc
     9241 cctgaacatc gcatcgcgaa ccgtcgattc gacgcagatg ctcgtcgtgc agcccgtgat
     9301 gatcaggtgc ttcgcgccga gctcacgcag ccgctcgtcg agatcggtct tgtaaaagcc
     9361 gctgaagcgc gttttgtaga gcacgatgtc gtcggcgtgc ggcttgagct cgtcgacgat
     9421 ctccgagttc caggtatcgc gcacgagaac gcggctcgga cgcccgtcgg gagccgtgac
     9481 gtgttggccc acaccgagcg cgaggtgcct gacgcggttg acggagtcct cgtggcccag
     9541 atccgacaag tcgggctggt aggccatctt gaggtagaca accttgatgc cggacttgcg
     9601 cgccgcgtca agcacgcgcg cagtcggcgg cacggccttc cgaataccgg agatgtcgat
     9661 gccggcgcgc tcgaacattc cgcctttgct gccgaaatcg ttctgcatgt cgacgacgat
     9721 gacagccgtc gtcgcggtat cgatggtgat cggctgcggc ttggcgtcga tcgtgacgcg
     9781 gcgacccgtg cggctttgag ccgctccctg gcccgcggcc aacgctgcga cgccggcagc
     9841 ggcaccgata cctacgacgt ctcggcgagt ta
//
LOCUS       DATLEE010000305         2326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9293497, whole genome shotgun sequence.
ACCESSION   DATLEE010000305 DATLEE010000000
VERSION     DATLEE010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9293497"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VM692_06480"
     CDS             complement(<1..627)
                     /locus_tag="VM692_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HUQ51850.1"
                     /translation="MGAVNSLGVNVAVALAFAAATTPTRAAETWGQALREGDVIVDLR
                     ARYESVDQVGIASEAGALTNRLRAGFQTAPLKGTAFLAEGVLVDDLVEDYNSTTNGYT
                     QYPVVADPADFAAINRLALINKSLERTTLTFGRQRIVHDDQRFVGNVGWRQNEQTFDG
                     LRAQWASPKLKTDLTYAAQVNRVFGPNSPQGKWKGDVVLANSAYTLPIG"
     gene            complement(962..1585)
                     /locus_tag="VM692_06485"
     CDS             complement(962..1585)
                     /locus_tag="VM692_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HUQ51851.1"
                     /translation="MRPRSLRILIVDENAVRAAILEEGLRVSSGREAGGALEIHNIRD
                     MKNLLPRIVGVDPDVILIDLENPSRDTLEQMFQVSRVAHRPIAMFVDKSDSATVQAAI
                     DAGVSAYVVDGLRKERVKSILDVTISRFHAFDRLQSELQQVKSALEDRKVIDQAKAIL
                     MKKRGCAEDEAYVLLRRTAMNQNRKIAELARSLVAAATLLGNGERDE"
     gene            complement(1740..>2326)
                     /locus_tag="VM692_06490"
     CDS             complement(1740..>2326)
                     /locus_tag="VM692_06490"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51852.1"
                     /translation="IVGRDRAEIARRLGPFRKRIRHDIIGVGRVQAVIVDRGIYMSVA
                     RTARVLTTACGLAALALVGTTAYAHHSFAMYDQTKQETYTGKLIRFIPGANHAQLLFE
                     LVGPDGQRQTGADGKPITWGVELGPAATIAKQGVTVEAFPLGTVITVTLNPLRNGKTF
                     GAMAQGARLIRCGTTIPAGGCTAETGEVFLESRN"
BASE COUNT          403 a          750 c          742 g          431 t
ORIGIN      
        1 gccgatcggc agcgtgtatg cagaattggc cagcacgacg tcccctttcc acttgccctg
       61 tggcgaattg gggccgaaca cgcggttgac ttgcgccgcg taggtcagat cggtcttgag
      121 cttcgggctg gcccactgag ctcgcagccc gtcgaacgtc tgttcgtttt gccgccaccc
      181 cacgttgccg acgaaccgtt ggtcgtcgtg aacgatgcgc tgccggccga acgtcaacgt
      241 cgttcgctcg agcgatttgt tgatcaacgc gagccgattg atggcggcga agtcggcggg
      301 atccgccacg acgggatact gcgtgtaccc gttcgtcgtg ctgttgtaat cctcgacgag
      361 atcgtcgacg agcacacctt cagccagaaa cgccgtgccc ttcagcggcg ccgtctgaaa
      421 accggcacgc agccgattcg tcagtgcacc tgcctcggac gcgatcccga cctgatcgac
      481 ggactcgtaa cgtgctctca ggtcgacgat gacatcgcct tcgcgcagcg cttgccccca
      541 ggtctccgcg gcacgcgtcg gcgtagtcgc agccgcaaac gccaacgcga ccgcaacatt
      601 caccccgagc gaattgacgg cacccatgct gaaactccct ttgttggtac gcacaaaaag
      661 aagccgctga gagggccgag cagtctcggc agcccttcag cggcgttgct ggtcgagccc
      721 aacgctggac tcgtcgtact gtgcgcaata caaacgccgt gccagcgacg cgtcagcgcg
      781 ggcgcggatg cctaacgcgt tgacgcttcg atgttttcga agcgcgcgtc gccgctacga
      841 agcgcacgcg cggcgcgcgt ctgcgccacg gtggttcagc ggcgcgttcg aacgccgcat
      901 agcggtgcaa cggcgaggat cagagcgcgt gcgcgcgtct ttgacgagcg ccacggcgcg
      961 ctcactcatc acgctcgccg ttgccgagca acgtggcagc ggcgacgagc gagcgcgcaa
     1021 gctcggcgat cttgcggttc tgattcatgg cggtgcggcg cagcagcaca taagcttcgt
     1081 cttcggcgca gccgcgcttc ttcatgagaa tggccttggc ttgatcgatg actttgcggt
     1141 cctcgagcgc cgacttgacc tgttgcagct cgctctgcag acggtcgaac gcatgaaagc
     1201 ggctgatcgt gacgtcgaga atcgacttca cgcgctcttt gcgtaaaccg tcgacgacgt
     1261 acgccgacac gccggcatcg atggcggctt gaaccgtcgc gctgtccgac ttgtcgacga
     1321 acatcgcgat cggccggtgg gcgacacgcg acacctgaaa catctgctcg agcgtgtcgc
     1381 ggctcgggtt ttcgagatcg atcagaatga cgtcgggatc gacgccgacg attcgcggca
     1441 gcaggttctt catgtcgcgg atgttgtgga tctcgagcgc gccgcctgcc tctctgccgc
     1501 tggaaacgcg taacccttcc tcgaggatcg ctgcacggac agcgttctcg tcgacgatga
     1561 gtattcgtaa tgaacgcggg cgcatggcac gaccgccaat gcaagaagcg tgcagaccgc
     1621 gctgcgccgc gccgcgcacg agtttttgct cgagcgacgc accaggacgg cacgcgcggc
     1681 gtagcgtgct gccccgttgt tgggcagggc tcggcaacgc tagctgcgga cgaaggcggt
     1741 tagttgcgcg actcgaggaa cacttcgccg gtctcggccg tgcagccgcc ggcgggaatc
     1801 gtggtgccgc aacggatcag gcgcgcacct tgcgccattg cgccgaacgt cttgccgttg
     1861 cgcaatgggt tcaacgtcac ggtaatgacg gtgccaagcg ggaacgcctc gacggtgacg
     1921 ccttgcttcg cgatcgtcgc tgcgggaccg agctccacgc cccacgtgat cggtttgccg
     1981 tcggcgccgg tctggcgctg cccgtcgggg ccgacgagct cgaacagcag ctgcgcatgg
     2041 ttcgctccgg gaatgaaccg gatcagcttg cccgtgtacg tctcttgctt cgtctggtcg
     2101 tacatcgcga acgagtgatg cgcgtaagcc gtcgtgccga ctagagccaa cgccgcgaga
     2161 ccgcaggcgg tcgtcaacac tctcgcggta cgtgcaacac tcatatatat acctctgtcg
     2221 acgatgacgg cctggacgcg gccgacgccg atgatgtcat ggcgaattcg tttgcggaaa
     2281 ggcccgagcc gccgcgcgat ctcggctcgg tcgcgcccga caatcg
//
LOCUS       DATLEE010000306         3878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1102494, whole genome shotgun sequence.
ACCESSION   DATLEE010000306 DATLEE010000000
VERSION     DATLEE010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102494"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VM692_06495"
     CDS             <1..1329
                     /locus_tag="VM692_06495"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HUQ51853.1"
                     /translation="TVRELAGDAWDELVGWDGKFLRTVRLLVTQPGALTRAAVEGRRA
                     RYVGPVRLYLICSVLYFVVSAVTPLPDVPVTVGPGVDLGLDAQRPEDAAFDKAFSQGL
                     ASLTPEERAIVDAEIEAQPAILQPFMRSQVEDFAAMQRRIGEVLPRALFLLVPALGAL
                     LALFYRRRHYPEHLYAAVHLQSFVFLVATLATLATLARFASSYAAIAAATVVAGAVIL
                     GHVMIAQRRVYEESWAEDGAQIRRGRTALFRALGPRLDGRIDLRRVRSRVTKRLLGAA
                     AMRALIRTMAVSAMLFVLTPASPQGVVTQRILSLQAAKVIAETALAACVSRGFHTSVV
                     VVDRAGNLLAVLRDELALPVTVEMARGKAYTAVVFRSSTLEFQENTADDPQRAPQRDV
                     PGILALAGGVPIVFEGEILGAVASSGSSQPTDDECAKAGAAKAESLLRAP"
     gene            complement(1361..2281)
                     /locus_tag="VM692_06500"
     CDS             complement(1361..2281)
                     /locus_tag="VM692_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ51854.1"
                     /translation="MLKPTVVIAGAAVLLAMANAGHAQFENVTPDMIRTALPVEGAPL
                     AEKGAYDVTSEPAFGSPGHVIVRPTNLAAFAKNDTLPVIAWGNGGCAINSTAFMGFMQ
                     TVASHGFLVIGTAAIEGAAQRRANADDLRGAIDWAAKENARDGSPLKGKIAMDHVAAM
                     GSSCGGFLSIEVGADPRVDTIGVFNSGVTRLDPKTPPPFPIPDVATLAKLHGPVLLVN
                     GHETDFMMATSKATYDWLENKPAFYGSRHGAGHSATFFHPGGGEFANVAWSWAAWQLK
                     GDRKAGSMFLGKDCGLCKNPNWDVESKGLK"
     gene            2421..>3878
                     /locus_tag="VM692_06505"
     CDS             2421..>3878
                     /locus_tag="VM692_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51855.1"
                     /translation="MLRTTLRRGALVLGATLAAAAGSAALAQSTAKAPAFTAAELVRQ
                     PAQNWVTNGGNIYNQRYSSLAQINRGNVAQVKAVWRTSLNGSGLGQGYSQQAQTLFYE
                     GALYVVTGDDDVFAVDVETGKILWTYEANVDFEHAVICCGRLSRGVGLGDGKVYVGRL
                     DSRLVALDQLTGKVLWDVAAGDPGISEGITAAPLYYDGKVIVGFTGGEYAVRGRIGAY
                     DAKTGKRIWNFYTVPGPGEIGHSTWPADNEAWKYGGAPVWQTPSVDPELGMIYFTTGN
                     AAPDLNGAIREGDNLFTVSLVALDANTGQYRWHFQLVRHDIWDFDAANPTILFDAEFD
                     GMRRKGITAVSKGGYLYILDRTSGVPLTPVVDTPVPQDKIQKTAATQPIPQGDQVVRH
                     EVDAVGENFEGILRNRARTFTPFSTGNPAIWRPFSGVTWHPSSYNPTNNLMYLCAGDG
                     PGRGTVGGDAAATIGPEPGAENRRYVQGTFGNARDL"
BASE COUNT          643 a         1327 c         1261 g          647 t
ORIGIN      
        1 accgtgcgcg agctcgccgg cgatgcctgg gacgagctcg tcggctggga cggcaagttc
       61 ctccgcaccg tgcggctgct cgtcacgcaa cccggtgcgc tgacgcgggc cgccgtcgag
      121 gggcgccgcg cgcgctacgt tggccccgtg cggctctatc tgatctgcag cgtgctctac
      181 ttcgtcgtat cggccgtcac gccgctgccc gacgtgccgg tgacggtggg cccaggcgtc
      241 gacctcgggc tcgacgcgca acgacccgag gatgccgcgt tcgacaaagc gttcagccaa
      301 ggcctcgcga gcctgacgcc tgaagaacgt gcgatcgtcg acgccgagat cgaggctcag
      361 cccgcgatct tgcagccgtt catgcgcagc caggtcgagg acttcgccgc tatgcagcgc
      421 cgcatcggcg aggtcctgcc gcgtgcacta tttctcctgg tgccggcgct cggggcgctg
      481 ctcgcgttgt tctatcggcg ccgccactat ccggagcacc tctacgccgc ggtccacctg
      541 caatccttcg tgttcctcgt cgcgacgctc gccacgctcg ccacgctcgc gcgctttgca
      601 agctcgtacg cggcgatcgc ggcggcgacc gtcgtagcag gagccgtcat cctcggccac
      661 gtgatgatcg cgcagcgccg tgtctacgag gagtcgtggg ccgaagacgg cgctcaaatc
      721 cgtcgcggtc gcactgctct atttcgtgct ttggggcctc gcctcgacgg gcgcattgat
      781 ctacgccggg ttcgttcgcg ggtgacaaaa cgattgttag gagcagcggc gatgcgagct
      841 ttgattcgaa cgatggcggt aagtgcgatg ctcttcgtct tgacgccggc gagcccgcaa
      901 ggcgtcgtga cgcaacgcat cctgtcgctg caagcagcga aggtcatcgc cgagaccgcg
      961 ctcgcggcgt gcgtgtcgcg cggctttcat acgagcgtcg tcgtcgtcga ccgcgccggc
     1021 aacctgctcg ccgtgttgcg tgacgagctt gcgcttcccg tcacggtcga gatggcgcgc
     1081 ggcaaggcgt acacggccgt cgtgtttcgc agctcgactc tagaatttca agagaacacg
     1141 gccgacgacc cgcagcgcgc tccgcagcgc gacgttccgg gcatcctcgc gctcgcgggc
     1201 ggcgtgccga tcgtcttcga aggcgagatc ctgggcgcgg tggcgagctc gggctcgagc
     1261 caacccacgg acgacgagtg cgcgaaagca ggcgcggcga aagccgaaag cctgctgcga
     1321 gcgccgtagc tccggccgtc cgcgccgcga ccaacgagcg ttacttcagc cccttggatt
     1381 cgacgtccca gttcggattc ttacagaggc cgcagtcctt accgaggaac atcgagcccg
     1441 ccttgcggtc gcccttgagc tgccaagccg cccagctcca ggccacgttc gcgaactcgc
     1501 cgccgcccgg gtggaagaac gtcgcgctgt ggcccgctcc gtggcgcgag ccgtagaacg
     1561 ccggcttgtt ctcgagccaa tcgtacgtgg ccttcgacgt cgccatcatg aagtcggtct
     1621 cgtggccgtt cacgagcagc acggggccgt gcagcttcgc gagcgtggcg acgtcgggaa
     1681 tggggaacgg cggcggcgtt ttcggatcga ggcgcgtcac acccgagttg aacacgccga
     1741 tcgtgtcgac gcgcgggtcg gcgcctacct cgatcgacag gaagccgccg cacgagctgc
     1801 ccatcgcagc cacgtggtcc atcgcgatct tgcccttgag cggcgacccg tcgcgcgcat
     1861 tctcctttgc cgcccagtcg atcgcgccgc gcaagtcatc ggcattcgcg cgccgctgag
     1921 ccgcaccttc tatcgccgcc gtgccgatca cgaggaagcc gtgcgacgcc accgtctgca
     1981 tgaaacccat gaaggccgtg ctgttgatcg cgcagccacc gttgccccac gcaatgacag
     2041 gcaacgtgtc gttctttgcg aacgcagcga gattcgtcgg acgcacgatg acgtggcccg
     2101 gcgagccgaa cgccggctcc gaggtgacgt cgtaggcccc cttctcggcg agcggcgcac
     2161 cttcgacggg cagcgcagtg cgaatcatgt ccggcgtgac gttctcgaat tgcgcgtggc
     2221 ccgcattggc cattgcgagc aaaacggcag cgccggcaat gacaacggtc ggcttgagca
     2281 tggtgttgtc ctcgagcgaa agcgaagtga tgccggactc accatagcca cggtcggctt
     2341 cgcgggcaac cctgcgcgga cgagtccgcc gccacgcgga tccctgggta tactgcagcg
     2401 catcgtcccg gggggaatcg atgcttcgta caacgctgcg ccgcggcgcc ctcgttctcg
     2461 gtgccactct cgccgccgca gccggctcgg ctgcgctcgc tcaatcaacc gcgaaagcac
     2521 cggcattcac cgccgccgag ctcgtgcgtc aaccggcgca gaactgggtc acgaacggcg
     2581 gcaacatcta taaccaacgc tactcgtcgc tggcccaaat caatcgcggc aacgtcgcgc
     2641 aagtcaaagc cgtctggcgc acgagcctga acggctcggg cctcggtcaa ggctactcgc
     2701 aacaagcgca aacccttttc tacgaaggcg ctctctatgt cgtcacgggc gacgacgacg
     2761 tgttcgccgt cgacgtcgag accggcaaga tcctttggac ttacgaagcg aacgtcgact
     2821 tcgagcacgc cgtcatctgc tgcgggcgct tgagccgcgg tgttggtttg ggcgacggca
     2881 aggtttacgt cggccggctc gacagccggc tcgtcgcgct cgatcagctc acgggcaaag
     2941 tcctctggga cgtcgcggcc ggcgatccgg gcatcagcga gggcatcacg gccgcgccgc
     3001 tctattacga cggcaaggtc atcgtcggct tcacgggcgg agagtacgcc gtgcgcggcc
     3061 gcatcggcgc ttacgacgcg aagaccggca agcgcatttg gaatttctac actgtgccgg
     3121 ggccgggcga gatcggccac tcgacgtggc cggcggacaa tgaggcatgg aaatacggcg
     3181 gcgcgcccgt gtggcagacg ccgagcgtcg accccgagct cggcatgatc tatttcacga
     3241 cgggcaacgc tgcacccgat ctcaacggcg cgattcgcga aggcgacaat ctcttcacgg
     3301 tgtcgctggt cgcgctcgac gcgaatacag gccaataccg ctggcacttc cagctcgtgc
     3361 gtcatgacat ctgggacttc gacgcggcga acccgactat tctgttcgac gccgagttcg
     3421 acggcatgcg ccgcaaaggc atcacggccg tgtcgaaggg cggctacctg tacatcctcg
     3481 atcgcacgag cggcgtgccg ctaacgcccg tcgtcgacac gccggtgccg caggacaaga
     3541 tccagaagac ggcggcaacg cagccgatcc cgcagggcga tcaagtcgtg cggcacgagg
     3601 tggacgccgt cggcgaaaac ttcgaaggaa ttctgcgcaa ccgcgcgcgg acctttacgc
     3661 cgttcagcac gggcaatccg gcgatctggc ggccgttctc gggcgtcacg tggcacccga
     3721 gctcgtacaa cccgacgaac aatctgatgt acctctgcgc cggcgacggc cccggccgcg
     3781 gtaccgtcgg cggcgatgcc gctgcgacga tcggccccga gcccggcgcc gagaaccgcc
     3841 gctacgtgca aggcacgttc ggcaatgcgc gcgacctc
//
LOCUS       DATLEE010000307         2085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10614490, whole genome shotgun sequence.
ACCESSION   DATLEE010000307 DATLEE010000000
VERSION     DATLEE010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10614490"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VM692_06510"
     CDS             <1..586
                     /locus_tag="VM692_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HUQ51856.1"
                     /translation="YGLAVSEAPAADLMLQAFGAPGRALIVAIVAVTSITSMNAILIA
                     GARTTYAAARDTTGLARLLGRWDVARGTPAAAIVATAGVALALVGFGAYTRGGFSTMV
                     DYLSPVYWLFMTLSGIALLVLRSRARDVPRPFRVPWYPFVPLAFVASSLYVLYSSLDY
                     VRVGAVAGIAVLLIGVALLAALRLTAKRSAPDAP"
     gene            complement(583..1152)
                     /locus_tag="VM692_06515"
     CDS             complement(583..1152)
                     /locus_tag="VM692_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HUQ51857.1"
                     /translation="MLFGLLVFALIVVVVFWFVATYNRLVAAAESATRAWNDLDALLR
                     QRHDEIPKVIEICEPHLKQERALFDRLLEARAAVFAARQARDADALNRAESSLRAAAT
                     QVVIERAAEVPELAASRAFALLRQRHATIDLELAERRDRYNAAVRDYNAVIGRLPGRL
                     VALLAEFPLLRPLDFESRPADEQGAPSRT"
     gene            1426..1776
                     /locus_tag="VM692_06520"
     CDS             1426..1776
                     /locus_tag="VM692_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUQ51858.1"
                     /translation="MTDSDDQLLAFGEFVRAQRRLAQVSQRNLARMSGVSDSYLSQIE
                     RGNYRPSPQVVKALATAFGLDAKQLYTMLGFMDEGDGNVAPSVEQAIQLDTRLNAEQK
                     ETLMRIYRSFVGEG"
     gene            1809..>2085
                     /locus_tag="VM692_06525"
     CDS             1809..>2085
                     /locus_tag="VM692_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51859.1"
                     /translation="MARDDNDVGQAIAEFQRRAREMMRAQQEAYLAAVKAWREGASKG
                     TPAWPEPSPPGLGPKPEELAEASYAFAAKLLADQSQFMQELSKTMAKN"
BASE COUNT          356 a          669 c          700 g          360 t
ORIGIN      
        1 ttacgggctc gcggtgagcg aggcgccggc ggccgacttg atgctgcaag cgttcggcgc
       61 gcccggccgg gcgctgatag tcgcgatcgt cgccgtgacg tcgatcacgt cgatgaacgc
      121 gatcttgatc gcgggggccc gaacgaccta cgcggctgct cgcgacacaa ccgggctcgc
      181 tcgcctgctc ggccgctggg acgtggcccg cggcacgccc gccgctgcga tcgtggcgac
      241 ggccggagtg gcgctcgcgc tcgtgggctt cggcgcttac acgcgcggcg gtttctcgac
      301 gatggtcgac tatctgtcgc ccgtgtactg gctgttcatg acgctgagcg gaatcgcgct
      361 gctcgtgctg cgcagccgtg ctcgagacgt gccgcggccg tttcgcgtgc cgtggtatcc
      421 gttcgtgccg ctcgcgttcg ttgcgagcag cctctacgtc ctctattcga gcttggacta
      481 cgtgcgtgtc ggcgccgtcg ccggcatcgc agtgctcttg atcggtgtcg cgttgctcgc
      541 ggcactgcga ctgaccgcca agcgcagcgc gcccgacgca ccctaagtcc tcgacggcgc
      601 cccttgttcg tccgcggggc gggactcgaa gtcgagcggg cgcagcagcg gaaactcggc
      661 tagtaatgca acgaggcggc ccggcagccg gccgatcacg gcgttgtagt cgcgcacggc
      721 ggcgttgtaa cggtcgcgtc gctccgcgag ctcgagatcg atagtcgcat gccgctggcg
      781 cagcaatgcg aacgcgcggc tcgctgcgag ctcgggcact tcggcggcgc gctcgatcac
      841 gacctgcgtc gcggcggcac gcagcgagct ttcggcgcga ttcagcgcat cggcatcgcg
      901 agcctgccgc gcggcgaaca cggcggcgcg cgcttcgagc aggcggtcga acagcgcgcg
      961 ctcttgcttc agatgcggct cgcagatctc gatgaccttg gggatctcgt cgtgccgctg
     1021 ccgcagcagc gcatcgaggt cgttccacgc tcgcgtcgcg ctctccgccg cggcgacgag
     1081 acggttgtaa gtggccacga accagaacac gaccactaca atgagagcga atacgaggag
     1141 gccgaacaac attcgtcttg ccgcttttgg atcaagcagt gcactgtaac tcgcgtcgcc
     1201 caatttggtg cgagcgcgac ggtgagccgc atcgtcttgg gcacgcgccc aagggtcagc
     1261 tactcggctg cgcgtcgctc gaagcgaatc gcccggcacg caattacgaa atgttgcaca
     1321 aaagcgccgg ccctggccat cgagcgcgta caatttcgcg cttgacgctt cggtcttcgg
     1381 cgccataatc caagcgaaac accgctgcgc cagcttggct cattcatgac cgacagcgac
     1441 gatcaacttc tggcctttgg cgagttcgtg cgtgcgcagc ggcggttggc acaggtttcg
     1501 caacgcaatc tggcgcgcat gtccggcgtt tccgattcgt atctgagcca gatcgagcgc
     1561 ggcaactatc ggccttcacc gcaagtcgtg aaggcgctgg cgaccgcgtt cggcctcgac
     1621 gcgaaacagc tctacaccat gctcggcttc atggatgagg gagacggcaa cgttgcgccg
     1681 agcgtcgagc aggccattca gctcgatact cgactcaatg cggaacagaa agagacgttg
     1741 atgcggattt accgctcgtt cgtcggcgaa ggctgattcg aacgggcaac gctcgccgga
     1801 gtatggagat ggcaagagac gataacgacg taggccaggc gatcgcggag tttcagcggc
     1861 gcgctcgcga aatgatgcgc gctcaacaag aggcgtacct cgcggccgtc aaggcttggc
     1921 gcgaaggagc gagcaagggc actccagcgt ggccggagcc gagcccgccg gggttgggcc
     1981 cgaaacccga ggagctcgcc gaggcctcat acgcgttcgc ggcgaagttg ctcgcggacc
     2041 agagccagtt catgcaggag ctcagcaaaa cgatggccaa gaaca
//
LOCUS       DATLEE010000308         3020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5575721, whole genome shotgun sequence.
ACCESSION   DATLEE010000308 DATLEE010000000
VERSION     DATLEE010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5575721"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VM692_06530"
     CDS             <1..905
                     /locus_tag="VM692_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ51860.1"
                     /translation="HVTVGGRETRDEKNGRLFTVNNLPTSLAFDIEDDHFDPMVTFAI
                     DATDNINLYAKYATGYRSGGASSRSVIYRSFGAEETDSIELGVKTELGNKLRLNAAAY
                     TMDRTGSQIDFSLVTPQPNGSTRNTLETINAPGTTKIKGLEVELTANLTSNLSVFGAY
                     AYTSTEVPPTVNPFNNVLQQVFIVFTPPRAASAGFDYEMPMKGGGMMMRAHFDGNYQS
                     AHHSLAEVAQKVDSSFIANARLSFENISVGRGNSELSVSLWSRNLLDEEHIYRLDPAN
                     RATLGDYANFSPPRTVGAEVSLSF"
     gene            complement(921..1769)
                     /locus_tag="VM692_06535"
     CDS             complement(921..1769)
                     /locus_tag="VM692_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HUQ51861.1"
                     /translation="MRRLARWLRRAAACAALLSWQAARADPAAIVNALRATGCCGVAA
                     VGAAAQRDTTLDAAAFEMGRDAKLADALERVGYPAAKSTAFHVRGSREDDVIRDMLAE
                     RYCAPINDPLYVALGVHQRGLDTWIIMAGRTAVPFTELRDPAAVAQRVFELINAARAE
                     ARQCGRERRAAAPPLTFSTTLAAAASLHALDMARRGVLSHEGADGSSSGERITRAGLA
                     WQASGENVAAGQPDADTVVQAWLDSPGHCATLMEPRFTQTGVAFALAPGKNPAVYWAQ
                     EFATPQ"
     gene            1935..2747
                     /locus_tag="VM692_06540"
     CDS             1935..2747
                     /locus_tag="VM692_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51862.1"
                     /translation="MATNRGLWLAVAFAAVAPLDAQWLVYPTAGVPRNADGSANLAAP
                     APRTPDGKPDFSGIWVSATLLPEGCGGPCIEQMPLPADAVDIGRTVDGGLPLQPWAAA
                     LSAERTKADSKDDPHARCLPPNFPRAYSFPQYKRVVQTKDLLVFLHEFNASFRQVHLD
                     GRPLPTDPEPTWNGYSTAHWERDTLVIESIGYRDELWLDLLGHPLTSRARITERMTRP
                     SYGTLAIELTVDDPGAYTRPWTAKLEQSIVLDTDIMDQVCLENEKSVRHMDP"
     gene            complement(2758..>3020)
                     /locus_tag="VM692_06545"
     CDS             complement(2758..>3020)
                     /locus_tag="VM692_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HUQ51863.1"
                     /translation="YVEKARQESAALGGRALEIRFEDLLREPERVVPEVARFCRVPAP
                     ANQRNLLEGLETGRAFAFRRDPELVAFAASVPEVLGRYGYAP"
BASE COUNT          522 a         1060 c          990 g          448 t
ORIGIN      
        1 tgcatgtaac ggtcggcggt cgcgagacgc gcgacgagaa gaacgggcgg ctgtttacgg
       61 tcaacaacct gccgacgagc ctcgcgttcg acatcgagga cgatcacttc gacccgatgg
      121 tgacgttcgc gatcgacgcg acggacaaca tcaacttgta cgcgaaatac gcgaccggct
      181 accgctccgg cggcgcgagc tcacgttcgg tgatctaccg ctcgttcggg gccgaagaaa
      241 ccgactccat cgagctcggc gtgaagacgg agctcggcaa caagctgcgg ctcaatgccg
      301 cggcctacac gatggaccgc acgggcagcc agatcgactt cagcctcgtg acgccgcaac
      361 cgaacggctc gacgcgcaac acgctcgaga cgatcaacgc gccgggcacg acgaagatca
      421 agggcctcga ggtcgagctg acggccaacc tgacgtcgaa cttgtccgtc ttcggggcgt
      481 atgcctacac ctcgaccgag gtgccgccga cggtcaatcc gttcaacaac gtgctgcaac
      541 aagtgttcat cgtcttcacg ccgccgcgcg ccgcgagcgc gggcttcgac tacgagatgc
      601 cgatgaaggg cggcggcatg atgatgcgcg cgcacttcga cgggaactat cagtctgcgc
      661 accacagcct tgccgaggtc gcgcagaagg tcgactcttc gttcatcgcc aacgcccgct
      721 tgtcgttcga gaacatctcg gtcggtcgcg gcaactccga gctctccgtc tcgttgtggt
      781 cgcgaaacct gttggacgag gagcacattt atcgcctcga tcccgccaat cgcgccacgc
      841 tcggcgacta tgcgaacttc agcccaccgc gaacggtcgg cgcggaggtg tctctgagct
      901 tctagcggcg gagctgacag ctactgcggc gtagcgaact cctgcgccca gtagaccgcc
      961 gggttcttgc ccggcgccag cgcgaaggca acgcccgttt gcgtgaagcg cggctccatc
     1021 agcgtcgcgc aatggcccgg gctgtcgagc cacgcctgca cgaccgtatc cgcgtcgggc
     1081 tgaccggcgg cgacattctc gcccgacgcc tgccaagcga gacccgcgcg cgtgatgcgc
     1141 tcgcccgacg agctgccgtc cgcgccttcg tggctcagca ccccgcggcg ggccatgtcg
     1201 agcgcatgca gcgaagcggc ggccgccagc gtcgtcgaga acgtcagcgg cggcgcagcc
     1261 gcgcggcgct cgcggccgca ctgcctcgct tcggctcgcg cggcattgat gagctcgaag
     1321 acgcgctgcg cgacggcggc cggatcgcgc aactcggtga acggtaccgc cgtgcggccg
     1381 gccatgatga tccacgtgtc caggcctcgc tgatgcacac cgagcgccac gtacaacgga
     1441 tcattgatcg gcgcgcagta gcgctcggcg agcatgtcgc ggatcacgtc gtcctcacgc
     1501 gagccgcgca cgtgaaacgc cgtcgacttc gcagcgggat agccgacgcg ttcgagcgcg
     1561 tcggccagct tcgcgtcgcg tcccatctcg aacgccgcgg catcgagtgt ggtgtcacgt
     1621 tgtgccgcgg cgccgacggc tgcgacgccg cagcaccccg tagcgcgcaa cgcgttgacg
     1681 attgccgccg ggtcggcgcg cgccgcttgc cacgagagca gcgcggcaca cgcagccgca
     1741 cggcggagcc agcgagcgag ccgcctcatg gtcctacgct aacgccagcc cgacgcggtg
     1801 ctcggccgcg cgagcgacga ggcgaagacg gcgtgaacga tgcgcatcgg cggccccccg
     1861 tgcaacggac gatgagtatt gcatggccgc ctcgcgcgcg tgttccgtta cagtagcggc
     1921 caatgggagg agccatggcg acgaacagag gcttgtggct tgcggtagca ttcgcggcgg
     1981 tggcgccgct cgacgcgcaa tggctcgtct atccgacggc cggcgtgccg cgtaacgccg
     2041 acggcagtgc gaacctcgcc gcgccggcgc cgcgcacgcc ggacggcaag cccgacttct
     2101 cgggcatctg ggtctcggcg acgctgctac ccgagggttg cggcggcccc tgcatcgagc
     2161 agatgccgtt acccgccgat gccgtggaca tcggccgcac ggtcgacggc ggcctgccgc
     2221 tgcagccttg ggccgcggcg ctctcggccg agcgcacgaa ggcggacagc aaggacgatc
     2281 cgcacgcgcg ctgcctgccg ccgaatttcc cacgcgccta ttcgttccct caatacaagc
     2341 gcgtcgtgca gactaaggac ctgctcgtgt tcctgcacga gttcaacgcg agcttccgcc
     2401 aggtgcacct cgacggccgg ccgttgccga cggatcccga gccgacctgg aacggctact
     2461 cgacggcgca ctgggagcgt gacacgctcg tcatcgaatc gatcggctat cgcgacgagc
     2521 tctggctcga cctcttgggc catccgctga caagccgcgc gcgcatcacc gagcgcatga
     2581 cgcgcccgag ctacggcact ctggcgatcg agctgaccgt cgacgatccc ggcgcctaca
     2641 cgcgcccgtg gaccgccaag ctcgagcaat cgatcgtgct cgacacggac atcatggatc
     2701 aggtgtgcct cgagaacgag aaatcggtcc ggcacatgga cccatagcgc accgcgctca
     2761 aggcgcgtag ccgtagcggc cgaggacctc gggcactgaa gccgcaaacg cgaccaactc
     2821 gggatcgcgg cggaatgcga aggcgcgacc cgtctcgagg ccctcgagca gattgcgctg
     2881 gttagccggc gccgggacgc gacagaatct tgcgacctcc ggcaccacgc gctcgggctc
     2941 ccgcaacagg tcttcgaagc gaatctcgag cgcgcgcccg ccgagggccg cggactcctg
     3001 tcgcgccttc tcgacgtatt
//
LOCUS       DATLEE010000309         4521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9869905, whole genome shotgun sequence.
ACCESSION   DATLEE010000309 DATLEE010000000
VERSION     DATLEE010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9869905"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VM692_06550"
     CDS             <1..780
                     /locus_tag="VM692_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51864.1"
                     /translation="LAAVARRTTAASHVMLLEGEGATREAVTSSLDSLRTRAKAADSV
                     VVMLVGHGSFDGEAYKLNLPGPDIAGEELAKLLSAVPARSQLIVNTTSASGAVLEKWA
                     ADGRTLITATRSGFERNATRFAQHFAAALAAGSADINKNGAITAQEAFDYASRSVADS
                     FEKDGALATEHPQIAGGAAGRFTVARLASAGPTVEASASNPAVAALVAEREKLDADIE
                     GLRARRESMANDAYLAELQKLLVQLAEVQGKIDAAQPRPQQ"
     gene            777..3080
                     /locus_tag="VM692_06555"
     CDS             777..3080
                     /locus_tag="VM692_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ51865.1"
                     /translation="MKLRGTLGSPRRGAAACAGLALCAASAVQALTIEYALDRAPALV
                     ACDEQANRGDRAGAAACYRALLAQSTDNRIKADAARATGDVRAANAFFQTAIKEYPED
                     AALRARWGELFVATHQNNEAVRLFEEALEIKEDYAPAKIGLAKVAAGRYEEKTREWAD
                     QVIADTPDQALEAHLMIARAALEDGAIDDGNAALDKAQKLASDHDRSPLEIYALRAAA
                     DLLRGTTDSPWTQRALALNSGYGNIYAIPAHFYVITRRYREAIALLERAVAIEPDLYE
                     AHAELGQNLLRENKIDEARPHLELAYRGDPFSAPNVNTLRLIDSFDNFVFSEHPGKRA
                     APGSNAANNPGAFLRLHKDEAAVLEPYVSALVDRTIDTYSKRYGFELKQPVIVELYPD
                     HDDFAVRIAGLPGIGLLGVTFGYLVAMDSPTGRADEHFHWGTTLWHEMAHVFTLEATN
                     HLVPRWFSEGVSVYEEWSTGPLPGRHIPLPVFAAIKEDKFLAVAELDRGFIRPTYEAQ
                     VIVSYMQAGLVCQYIAGRFGQEALERMLIEFRNGKDTAQAIQGALGISPTQFDENFAA
                     YVETELGTVVAGLERWQEKQAEVIKSVEDSDWRLVVAKAAEAIELFPDYVDEGSPYIA
                     KARAHRELGETVLMTNTLLEYRKRGGYEPDALITLARTLGEAGRNDDALDVFKDVIMV
                     APLRPQVHLEYGDRLTAANRPAEALIEYQALLAMNPQDLAEAHYRIAKTYVALEDKAK
                     GREHLLYALEIAPHYREAQQLLLEVVR"
     gene            3077..4117
                     /locus_tag="VM692_06560"
     CDS             3077..4117
                     /locus_tag="VM692_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HUQ51866.1"
                     /translation="MSDATSNLDTQETRALIDNFKSSFGQIRTEVRKIIVGQDDVVEH
                     LLMTLLVGGHCLITGMPGTAKTLLVRTLASALGLTFKRIQFTPDLMPTDVTGTDILEE
                     DQSTGRRTWTFVPGPLFTNVLLADEINRTPPKTQSALLEAMQEFSVTVRGTHHKIDRP
                     FFVLATQNPIELEGTYPLPEAQLDRFLFNVLLDYLSAEDEVDVVTRNTTRMELPEVNA
                     CTTPEQILKFQWLVRQVPVSQVASRYAVDLVRATRPHDPNASEMVKKYVQYGASVRAT
                     QFITLAAKARALMQGRYHVTHEDIASLALPVMRHRVLTNYFAESDGIDTDKVLADLVK
                     HVGADTRQARVG"
     gene            4123..>4521
                     /locus_tag="VM692_06565"
     CDS             4123..>4521
                     /locus_tag="VM692_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUQ51867.1"
                     /translation="MGAGTAVPTHLLAPETLAGLANLDLVARAVVNGFLTGMHRSPFF
                     GFSQEFAEYRAYAEGDDPRFVDWNVYARTDRTYIKRYIGETNTRLVILLDASASMGFG
                     SGKVNKLLYGKFLAATLAYLAARQHDAVGLI"
BASE COUNT          822 a         1540 c         1477 g          682 t
ORIGIN      
        1 ctcgccgcgg tcgcgcgccg cacgacggcc gcgagccacg tcatgctgct cgaaggcgag
       61 ggcgccacgc gcgaagccgt gaccagcagc ctcgactcgt tgcggacgcg cgccaaagcg
      121 gccgacagcg tcgtcgtcat gctcgtcggc cacggcagct tcgacggcga agcatacaag
      181 ctgaacttgc ccgggcccga catcgccggc gaggagctcg cgaagctgct ctccgccgtg
      241 ccggcgcgct cgcagctcat cgtcaacacg acgagcgcca gcggcgccgt gctcgagaaa
      301 tgggcggccg acgggcgaac gctcatcacg gcgacgcgca gcggcttcga gcgcaatgcc
      361 acgcgcttcg cgcagcattt cgccgcggcg ctggccgcgg gctcggccga catcaacaag
      421 aacggtgcga tcacggctca agaggcgttc gattatgcct cgcgcagcgt cgccgatagc
      481 ttcgagaagg acggcgcgct cgcgacggag cacccgcaga tcgccggcgg tgcggccggc
      541 cgcttcacgg ttgcgcgctt ggcatccgca gggccgaccg tcgaggcgtc ggcgtccaac
      601 ccggccgtgg cggcgctcgt cgccgagcgc gagaagctcg acgccgacat cgaaggcttg
      661 cgtgcgcgcc gcgaatcgat ggccaacgac gcctatctcg ccgagcttca gaagctgctc
      721 gtccagctcg cggaggtcca gggcaagatc gacgccgcgc agccgaggcc gcagcagtga
      781 agctgcgagg gactcttgga agtccccgaa ggggggccgc cgcttgcgcc ggcctggcgc
      841 tctgcgccgc gtcggcggta caggcgctga cgatcgagta cgcgctcgac cgcgccccgg
      901 cgctcgtcgc ctgcgacgag caagccaatc gcggcgaccg agcaggcgcc gccgcgtgct
      961 atcgcgccct gctcgcgcaa agcacggata accgcatcaa ggccgacgcc gcgcgcgcga
     1021 ccggcgacgt gcgcgccgcc aatgcgttct tccagacggc gatcaaggaa taccccgagg
     1081 atgcggcgct gcgcgcgcgt tggggcgagc tgttcgtcgc gacgcatcag aacaacgagg
     1141 ccgtgcgcct gttcgaggaa gcgctcgaga tcaaggagga ctacgcgccg gccaagatcg
     1201 gcctcgcgaa ggtcgcggcc gggcgctacg aggagaagac gcgcgagtgg gccgaccagg
     1261 tcatcgccga cacgccggac caagcgctcg aagctcacct catgatcgcg cgcgcggctc
     1321 tcgaggacgg cgccatcgat gacggcaacg cggcgctcga caaggcccaa aagctcgctt
     1381 cagatcacga ccgttcgccg ctcgaaatct acgcgctgcg agccgccgcc gatttgctgc
     1441 gcggcacgac ggacagcccg tggacgcagc gcgcgctcgc gctgaactcg gggtacggca
     1501 acatctacgc gatccctgcg cacttctacg tcatcacgcg ccgttatcgc gaagccatcg
     1561 cgctgctcga gcgcgcagtc gcgatcgagc ccgatctcta cgaggcgcac gcggagctcg
     1621 gccaaaatct gctgcgcgag aacaagatcg acgaggcgcg gccgcacctc gagctcgcgt
     1681 atcgcggcga cccgttcagc gcgccgaacg tcaacacgct gcggctcatc gacagcttcg
     1741 acaacttcgt cttctcggag cacccgggga aacgtgcggc gcccggttcg aacgccgcca
     1801 acaatcccgg cgcgttcttg cggctgcaca aggacgaagc cgcggtgctc gagccgtacg
     1861 tttcggcgct cgtcgatcgc acgatcgaca cgtacagcaa gcgctacggc ttcgagctca
     1921 agcaacccgt gatcgtcgag ctctaccccg accatgacga tttcgcggtg cgcatcgccg
     1981 gcttgcccgg catcgggctc ctcggcgtga cgttcggcta tctcgtcgca atggacagcc
     2041 cgacgggccg ggccgacgag cacttccact ggggcacgac gctgtggcac gagatggcgc
     2101 acgtgttcac gctcgaggcc acgaaccatc tcgtgccgcg ctggttcagc gaaggcgtct
     2161 cggtctacga ggagtggagc acggggccgt tgccgggccg gcacattcca ctgcccgtgt
     2221 tcgccgcgat caaagaggac aagttcttgg ccgtagcgga gctcgaccgc ggcttcatcc
     2281 ggccgacgta cgaagctcag gtcatcgtgt cgtacatgca ggcggggctc gtctgtcagt
     2341 acatcgccgg gcgattcggg caagaggcgc tcgagcgaat gctaatcgag ttccgcaacg
     2401 gtaaggacac ggcgcaagcg attcagggcg cgctcggcat ctcgccgacg cagttcgacg
     2461 agaacttcgc tgcctacgtc gagaccgagc tcggcaccgt ggtcgcgggg ctcgagcggt
     2521 ggcaggaaaa acaggccgag gtcatcaaga gcgtcgagga ctcggattgg cggctcgtcg
     2581 tcgcaaaagc cgccgaggcc atcgagctct tccccgacta cgtcgacgaa ggcagcccgt
     2641 acatcgccaa ggcgcgcgcg catcgcgagc tcggcgagac ggtgctcatg acgaacacgc
     2701 tgctcgagta tcgcaagcgc ggcggctacg agcccgacgc gttgatcacg ctcgcgcgca
     2761 cgctcggcga agcgggccgc aacgacgatg cgctcgatgt gttcaaggac gtcatcatgg
     2821 tcgcgccgct gcggccgcaa gtgcatctcg agtacggtga ccggctgacg gcggcgaatc
     2881 gcccggccga agcgctcatc gaataccagg cgctgctcgc gatgaatccg caggatctcg
     2941 ccgaggcgca ttaccgcatc gcgaagacct acgtggcgct cgaggacaaa gccaagggcc
     3001 gcgaacattt actgtatgct ctggaaatcg ccccgcatta tcgggaagcc caacagctgc
     3061 tcttggaggt cgtacgttga gcgacgcaac tagcaatctc gacacgcaag aaacgcgtgc
     3121 gctgatcgat aatttcaaat cgtcctttgg gcagatccgc accgaagttc gcaagatcat
     3181 cgtcggccag gacgacgtcg tcgagcatct gttgatgacg ttgctcgtcg gcggccactg
     3241 cttgatcacg ggcatgccgg gcaccgccaa gacactgctc gtgcgcacgc ttgcgagcgc
     3301 gctgggcctc acgttcaaac gcattcagtt cacgccggac ttgatgccga cggacgtcac
     3361 gggcaccgac atcctcgagg aggatcaaag cacggggcgc cgcacgtgga cgttcgtgcc
     3421 gggcccgttg ttcacgaacg tgctgctcgc cgacgaaatc aatcgcacgc cgccgaaaac
     3481 gcagtcggcg ttgctcgagg caatgcaaga gttctcggtg accgtacgcg gcacgcacca
     3541 caagatcgac cggccgttct tcgtgctcgc cacgcagaac ccgatcgagc tcgagggcac
     3601 gtacccgctg cccgaggccc agctcgaccg cttcctgttc aacgtgctgc tcgactactt
     3661 gagcgccgag gacgaggtcg acgtcgtgac gcgtaacacg acgcgcatgg agttgcccga
     3721 ggtcaacgcg tgcactacgc cggagcagat cctgaagttc cagtggctcg tgcgccaagt
     3781 tccggtctcg caggtcgcga gccgctatgc cgtcgacctc gtgcgcgcga cgcgcccgca
     3841 cgatccgaac gcgtccgaga tggtcaagaa gtacgtccag tacggcgcga gcgttcgcgc
     3901 gacgcagttc atcacgttgg cggccaaggc tcgcgcgctg atgcagggcc gctatcacgt
     3961 cacgcacgag gacatcgcgt cgctggcgtt gcccgtcatg cgccatcgcg tgctgaccaa
     4021 ctacttcgcc gagtccgacg gtatcgatac cgacaaggtg ctggcggacc tcgtcaagca
     4081 cgtcggtgcg gataccaggc aggcccgcgt aggctgaccg tcatgggcgc agggacggcg
     4141 gtaccgacgc atctgctcgc acccgagacg ctcgcgggcc tcgcgaacct ggacctcgtc
     4201 gcgcgcgccg tcgtcaacgg cttcctgacc ggcatgcatc gctcgccgtt tttcggcttc
     4261 agccaggagt tcgccgagta tcgcgcctac gccgaaggcg acgacccgcg cttcgtcgac
     4321 tggaacgtct acgcgcggac cgaccgcacc tacatcaagc gttacatcgg cgagacgaac
     4381 acgcggctcg tgatcctgct cgacgccagc gcgtcgatgg gcttcggcag cggcaaggtc
     4441 aacaagctgc tgtacggcaa gtttctggcc gcgacgctcg cgtacttggc cgcgcgccag
     4501 cacgacgccg tgggcttgat c
//
LOCUS       DATLEE010000310         4702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9296823, whole genome shotgun sequence.
ACCESSION   DATLEE010000310 DATLEE010000000
VERSION     DATLEE010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9296823"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VM692_06570"
     CDS             <1..1127
                     /locus_tag="VM692_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUQ51868.1"
                     /translation="SVAEIDRGAAQSDGMVGIEIPVEVLADDNDKHIELRYRPLGVVA
                     IITPWNAPVNLALGPLVSALYTGNTVILKPSPYTPLTTLKLGEIMRDIFPAGVVNVLA
                     GGDELGAAMTQHAGIDKISFTGSVATGKKVLASASATLKRVTLELGGNDPAIVMGDVD
                     AKAVAKKIFFASFVNSGQVCMAIKRIYAHESVYDELCAALVDEAKKAKVGNGLDPATE
                     LGPVQNKMQYDKVVGLIEDTKRAGATFLAGGEIPEGPGYFLPPTLVTDVADDSRLVQE
                     EQFGPIVPILKFTELEDALRRANDTRYGLSGSVWTKDVQRGAEIAARLEVGTAWVNQH
                     RTTSAFVPFGGAKESGLGRQYSALGLKSYMEPEVVSVLKR"
     gene            complement(1108..2607)
                     /locus_tag="VM692_06575"
     CDS             complement(1108..2607)
                     /locus_tag="VM692_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51869.1"
                     /translation="MRTWLIAAALLILPLFIYWPTISHEYGFRDDYAHLREVRERPGW
                     LLQLTSSNGRPVYGLVLEASLAEVYQVPELEALRSAAAALIGVVGLLLWWHLRRSGWS
                     EAQAAALGAAAMLLPGVQIVVGWAIAWPIALALVTAVAGFAFVDRALELRGRARMVGL
                     AAGCAAYFVAGLTYQTSALFAVMPLAAVLLRREQGTARGDVEWTFLHLAALFGGLFAG
                     FLLMNLVFTEGVVAEADRMQIEPHPFIKLLWFLRNPLPNSLALFALRDSYATPFWFWF
                     VVAAVVAVAVLGFVCISKDGRQRQRWLFAALVLPFAAHSVSLAASSQAIGYRTLLPMS
                     GLFLVLFVFGVRALLAHYKAPRVVGPVLLGVLVLVGAVLARHNALTLIAEPQGNEWKL
                     FQAAASRLPIGRETSVYITRPTYSYRSTQRVYADEYGSLSADAQWAAVEMFRAAVRQR
                     FPQGLPEGTTYTITTSLTPPIKGYDLVLDLRQLRTLGEHAPQETTASER"
     gene            complement(2609..3307)
                     /locus_tag="VM692_06580"
     CDS             complement(2609..3307)
                     /locus_tag="VM692_06580"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ51870.1"
                     /translation="MDEVEFDKFADEYRDLHAANIKLSGENPEYFAEYKVADIAAELE
                     RDRAVAHTALDFGAGVGYSVPFFARHLPAARVTCLDVSRKSLELGAARHGAAAEFRHF
                     DGRSIPYADGTFDVALASCVFHHIPHAEHVALLAEIRRVLTPAGRLFVFEHNPLNPLT
                     RHAVNTCVFDEHAELIMAPTMRRRMRAAGFASADVRYRIFFPHALRGLRPLEAKLTWL
                     PLGAQYYVAARKSH"
     gene            complement(3316..4158)
                     /locus_tag="VM692_06585"
     CDS             complement(3316..4158)
                     /locus_tag="VM692_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HUQ51871.1"
                     /translation="MSSTSRLIVNADDFGIAEAVNRGIAAAFDRGIVTSTSIMATGPA
                     FEHAVELARSRPGLAVGIHLVLTEHRPLIGAAAAASLVEPDGRFPQHVAPLLRKQLRG
                     RIRLAEVRLELEAQLERVRKAGVVLSHIDGHQHVHVLPGIAGVVAELADRHGLAAVRY
                     PAERVRGYMLRSLKHARRVAEQAALRLFCESSPLKHLRRSDDFVGFYFGGRLDEANLA
                     TVLAGLPAGRTVELMCHPGDEDMQGADEWVYAWSAERDALTSPRIRDLVAARGLELIS
                     YRDV"
     gene            complement(4155..4562)
                     /locus_tag="VM692_06590"
     CDS             complement(4155..4562)
                     /locus_tag="VM692_06590"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HUQ51872.1"
                     /translation="MPGFGLRYPLARFVVVGCANFVVSFTAFYAAYQWLPLAESGGRG
                     ATANVLAYAAGMINSFVLNRLWTFRAEGQVAVHAAKFIVLNAATLIGSTAIVLVLVDR
                     AGFPALAVWLPLTLAILTAHYLGMKHWAFAGRK"
BASE COUNT          871 a         1603 c         1570 g          658 t
ORIGIN      
        1 aatccgtcgc cgagatcgac cgcggcgccg cgcaatcgga cggcatggtc ggcatcgaga
       61 ttcccgtgga ggttctagcc gacgacaacg acaagcacat cgagctcagg tatcggccgc
      121 tcggcgtcgt cgccatcatc acgccgtgga acgcgcccgt gaatctcgcg ttgggcccgc
      181 tcgtatcggc gctgtacacg ggcaacaccg taatcctcaa accttcgccg tacacgccgc
      241 tgacgacgct gaagctcggc gagatcatgc gcgacatctt ccccgccggc gtcgtcaacg
      301 tgctggccgg cggcgacgag ctcggcgccg cgatgacgca acacgccggc atcgacaaga
      361 tttcgttcac gggctccgtg gcgaccggca agaaagtgct cgcgagcgcc tcggcgacgc
      421 tgaagcgggt gacgctcgag ctcggcggca acgatcccgc gatcgtcatg ggcgacgtcg
      481 acgcgaaagc cgttgcgaag aagatcttct tcgcttcgtt cgtcaacagc ggccaggtct
      541 gcatggccat caagcgaatc tacgcgcacg agtccgtcta cgacgagctg tgcgccgcgc
      601 tcgtcgacga ggccaagaag gctaaggtcg gcaacggcct cgaccccgcg acggagctcg
      661 gccccgtgca gaacaagatg cagtacgaca aggtcgtcgg cctcatcgag gacacgaagc
      721 gagcgggcgc cacgttcctc gccggcggcg agattcctga gggccccggc tacttcctgc
      781 cgccgacgct cgtcaccgac gtcgccgacg acagccggct cgtgcaagaa gagcaattcg
      841 gcccgatcgt gccgatcctg aagttcaccg agctcgagga cgcgctgcgc cgcgctaacg
      901 acacgcgcta cggcctgagc ggctccgtct ggaccaagga cgtgcagcgc ggcgccgaga
      961 tcgcggcgcg gctcgaggtc gggacggctt gggtcaacca gcaccgcacg acgtcggcgt
     1021 tcgtgccgtt cggcggcgcg aaggagtcgg ggctcgggcg gcaatattct gcgctcgggc
     1081 tcaagagcta catggagccc gaggtcgtca gcgttctgaa gcggtagtct cttgcggcgc
     1141 gtgctcgccc aaagtcctga gctgccgcaa atcgagcact agatcgtagc ccttgatcgg
     1201 cggcgtcagg ctcgtcgtga tcgtgtaggt cgtaccctcc ggcaagcctt gcgggaagcg
     1261 ttgccgcacg gcggccctga acatctcgac ggcggcccac tgcgcgtcgg ccgacaacga
     1321 gccgtactcg tcggcataaa cgcgttgcgt cgagcggtag gagtaggtcg gccgcgtgat
     1381 gtagacgctc gtctcgcggc cgatcggcaa gcggctcgcg gcagcttgaa acagcttcca
     1441 ctcgttgcct tgcggctcgg caatcaacgt caacgcgttg tggcgcgcga gcacggcgcc
     1501 gacgagcacg agcacgccga gcagcaccgg ccctacgacg cgcggcgctt tgtaatgcgc
     1561 gagcagcgct cgcaccccga acacgaacag cacgagaaac aaccccgaca tcggcagcag
     1621 cgttcgatag ccgatggctt gcgagctcgc ggcgaggctg acgctgtgag ccgcgaacgg
     1681 cagcaccaac gccgcgaaca accaacgttg tcgctgccgc ccgtccttgg agatacatac
     1741 gaagccgagc accgcgacgg cgacgaccgc cgcaacgacg aaccagaacc agaacggcgt
     1801 cgcatagctg tcgcgcagcg cgaacagcgc gagggagttc ggcaacggat tgcgcaggaa
     1861 ccacagcagc ttgatgaacg gatgcggctc gatctgcatg cggtccgctt cggcgacgac
     1921 gccctcggtg aacacgaggt tcatcaacaa gaaacctgcg aacagcccgc cgaacagcgc
     1981 ggctaggtgc aggaacgtcc actccacgtc gccgcgcgcc gtgccctgct cgcgccgcag
     2041 caacacggcg gccagcggca tcacggcgaa cagcgcgctc gtttgatacg tgagcccggc
     2101 gacgaagtac gctgcgcacc ccgccgccag gcccaccatg cgcgcgcggc cgcgcagctc
     2161 gagcgcacgg tcgacgaagg cgaacccggc gacggccgtg acaagcgcga gcgcaatcgg
     2221 ccaggcgatg gcccagccga cgacgatctg cacgccgggc agcaacatcg ccgccgcgcc
     2281 caacgcggcg gcttgcgcct cgctccagcc ggaacgccgc aagtgccacc agagcagcag
     2341 gccgacgacg ccgatcagtg ccgcggccgc ggaacgcagc gcttcgagct ccggcacctg
     2401 ataaacctcg gcgagcgagg cctcgagcac gagaccgtaa acgggccggc cgttcgacga
     2461 cgtcagctgc agcagccagc ccggccgttc ccggacctcg cgcagatgcg cgtagtcgtc
     2521 acggaaccca tactcgtgtg aaatggttgg ccagtagatg aataacggaa ggatcagcag
     2581 cgcggcggcg atcagccatg tacgcatgct aatgagactt gcgcgccgcg acgtagtact
     2641 gcgcgcctag cggcagccac gtgagcttcg cttcgagcgg gcgcagcccg cgcagcgcat
     2701 gcgggaagaa aatgcgataa cggacgtcgg ccgacgcgaa gccggcagcg cgcatgcgcc
     2761 gccgcatcgt cggcgccatg atgagctcgg catgctcgtc gaacacgcat gtgttgacgg
     2821 cgtgccgcgt cagcgggttc aaagggttgt gctcgaacac gaacagccgg ccggccggcg
     2881 tcagaacgcg ccgtatctcg gcgagcagcg cgacgtgctc cgcgtgcgga atgtgatgga
     2941 acacacacga cgcaagcgcg acgtcgaacg tgccgtcggc gtacggaatg cttcggccat
     3001 cgaagtgtcg gaactcggcc gcggcaccgt gccgcgccgc gccgagctct aaacttttgc
     3061 gcgagacgtc gaggcaggtg acgcgcgccg ccggcaagtg gcgcgcgaag aacggcacgg
     3121 aatagccgac gccggcgccg aagtcgagcg ctgtgtgcgc gacggcacga tcgcgctcga
     3181 gctcggccgc aatgtcggcg accttgtact cggcgaaata ctcggggttc tcgcccgata
     3241 gcttgatgtt ggccgcgtgc aggtcgcggt actcgtcggc aaacttgtcg aactcgacct
     3301 cgtccatgac tccgctcaaa cgtctctata agaaatgagc tcgagaccgc gagccgccac
     3361 gaggtcgcgg atacgcgggc tcgtcagcgc gtcgcgctcc gccgaccaag cgtagaccca
     3421 ttcgtcggcg ccctgcatgt cctcgtcccc cgggtggcac atcagctcga cggtgcgtcc
     3481 ggccggcagg ccggccagca cggtcgcgag attcgcttcg tcgaggcggc cgccgaagta
     3541 gaagcctacg aaatcgtcgc tgcgccgcaa gtgcttcagc ggcgaggact cgcaaaacag
     3601 tcgcagcgcc gcctgctccg cgacgcgccg cgcatgcttc aagctgcgca gcatgtagcc
     3661 gcgaacgcgc tcggccggat aacggacggc cgctaagccg tgacggtccg caagctcggc
     3721 cacgacgcct gcgatgcccg gcagcacgtg cacgtgctgg tgaccgtcga tatggctcag
     3781 cacgacgccg gctttgcgca cgcgttcgag ctgcgcctcg agctcgaggc ggacctccgc
     3841 cagcctgatt cggccgcgca attgcttgcg caacagcggc gcgacgtgct gcggaaagcg
     3901 gccgtcgggc tccacgaggc tggccgcggc cgccgccccg atcagcgggc ggtgctcggt
     3961 cagcacgagg tgaatgccga cggcgagccc gggccggctg cgcgcgagct cgacggcatg
     4021 ctcgaacgcg ggccccgtcg ccatgatcga cgtcgaagtc acgatgccgc ggtcgaatgc
     4081 cgctgcgata ccgcggttta cggcctccgc gatgccgaag tcgtccgcgt tgacgatgag
     4141 cctcgacgtc gagctcattt gcgccccgcg aacgcccagt gcttcatgcc gaggtagtgc
     4201 gccgtcagga tcgcgagcgt cagcggcaac caaacggcga gcgccgggaa cccggcgcgg
     4261 tcgacgagca cgagcacgat ggccgtgctg cctatcagcg ttgcggcatt gagcacgatg
     4321 aactttgccg cgtggacggc gacctgacct tcggcgcgaa acgtccagag ccgattcagc
     4381 acgaagctgt tgatcatgcc ggcggcatac gccagcacgt tggccgttgc gccgcggccg
     4441 ccggactcgg caagcggcag ccattgatac gctgcgtaga acgccgtgaa gctcacgacg
     4501 aagttcgcgc agccgacgac gacgaaacgt gccagcggat atcgcaagcc gaagccgggc
     4561 acctggttca gctcagcctt cgcgccgctg cgcgcgccta tcggcggccg cgagcacggg
     4621 ccgcgtcgtc tcgcggacga ggaaccgcgg gcggcggcgc acctcgacgg cgatgcgccc
     4681 gacgtattcc gcgagcacgg ct
//
LOCUS       DATLEE010000311         8368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9870370, whole genome shotgun sequence.
ACCESSION   DATLEE010000311 DATLEE010000000
VERSION     DATLEE010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9870370"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..91)
                     /gene="rpmE"
                     /locus_tag="VM692_06595"
     CDS             complement(<1..91)
                     /gene="rpmE"
                     /locus_tag="VM692_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HUQ51873.1"
                     /translation="MKPEIHPTYTAIKVICSCGNEFTTRSTLGQ"
     gene            complement(424..1029)
                     /gene="rdgB"
                     /locus_tag="VM692_06600"
     CDS             complement(424..1029)
                     /gene="rdgB"
                     /locus_tag="VM692_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012847150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HUQ51874.1"
                     /translation="MTLPAHWVLATSNRGKVAELGALLAEAGLAVRVTAQSELNVIPA
                     PETAVTFVENALAKARHAARVTGLPAIADDSGLVVDALGGAPGVRSARFAGEDADDGA
                     NVAKLLTLLAGQALERRAHFHCVLVALTSPDDAAPLIASGSWHGEIARAARGSNGFGY
                     DPVFFDPTLRCTAAQLGAAEKNRVSHRGTALRRLIELLKNR"
     gene            complement(1026..1742)
                     /gene="rph"
                     /locus_tag="VM692_06605"
     CDS             complement(1026..1742)
                     /gene="rph"
                     /locus_tag="VM692_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HUQ51875.1"
                     /translation="MRPSGRRRDEMRHVQLTPHFTRHAEGSVLAEFGDTRVLCTASVE
                     PKVPPFLKGKAQGWVTAEYGMLPRATHSRTPREAARGTQSGRTLEIQRLIGRSLRAVV
                     DRDALGEHTITIDCDVLQADGGTRTAAITGGYVALALAAAQLAKNGKISRNPIHGQVA
                     AVSVGIFRGQAVLDLDYAEDSEAETDMNVVMNGGKGFIEVQGTAEGHAFQRSELNTLL
                     DLAGVGIERLLAAQAAALGK"
     gene            complement(1804..2547)
                     /locus_tag="VM692_06610"
     CDS             complement(1804..2547)
                     /locus_tag="VM692_06610"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HUQ51876.1"
                     /translation="MRVDYAKLTLPGNRTENQDRVDLIASGESLLIVVVDGMGGHAHG
                     ARAAEVTVATLKESFAEMSLPIFDPQGFLTLSLARAHDRVVKLGDGVALDHKPRATCA
                     VCLVQDGGAYWAHVGDSRIYQLRGGHIQARTRDHSHVELLLREGLIAESEMRNHPMRN
                     FVECCLGGDVPLPDMSVTARNKLEPNDVLLVCTDGLWSGIEDEDLAAANEPGTSIETA
                     IRALADRAVVNNSPHSDNTSVAAVRWQGP"
     gene            2716..3570
                     /locus_tag="VM692_06615"
     CDS             2716..3570
                     /locus_tag="VM692_06615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HUQ51877.1"
                     /translation="MTGFSTAEARVGAFRLVWEIRSVNHRFLELGFRLPEDLRAVEPE
                     CRELVGTTIKRGKVDCTLKISADERSATADTIVAGALERLRKLEEQVRHVFRDAPPMS
                     AQEVLRWPGVLEEPAQNLEELGEAVRACLGVALRALTDARAREGGRIAEMLEKRNAGI
                     TALITGIKPQLDGLQLRYRDRLRERLQRLDVQADPERLEQELALIAQRVDVAEEIDRL
                     ASHVAEIQAILHRNEPIGRRLDFVIQELNREANTFGSKVQEEALTRVAVELKVLIEQM
                     REQVQNLE"
     gene            3567..4211
                     /gene="gmk"
                     /locus_tag="VM692_06620"
     CDS             3567..4211
                     /gene="gmk"
                     /locus_tag="VM692_06620"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019024931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HUQ51878.1"
                     /translation="MSATPQRTGRLWVVAAPSGAGKTTLVRALLERDPLLRFSTSYTT
                     RPPRSSEVNGKDYFFVDVPQFEAMVRDDAFLEHAQVFDNWYGTGRDHVASLLAAGFSV
                     VLEIDWQGAQQVRKRAPDSQSIFILPPSVGELERRLRGRKTDSEAVIQRRLKDSLADM
                     GHWSEFDHVIVNEDFAAALDRLAAVVAGREHGARTSLPAVRAAVADIVSGRPKT"
     gene            4280..4579
                     /gene="rpoZ"
                     /locus_tag="VM692_06625"
     CDS             4280..4579
                     /gene="rpoZ"
                     /locus_tag="VM692_06625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HUQ51879.1"
                     /translation="MARITVEDCLEKVPNMFQLVLVAAKRARQLANGAHPMVDWENDK
                     PTVVALREIAEGYITEEILTQRDKPVEDILAADLAAVTPMSPDELLGTRPVSLGD"
     gene            4626..6782
                     /locus_tag="VM692_06630"
     CDS             4626..6782
                     /locus_tag="VM692_06630"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HUQ51880.1"
                     /translation="MDYGAATLDRIVSSVWRTDDFGRLMGQLERYLPSNDLPSITAAY
                     EFSAKAHQGQQRRNGDPYISHPVAVAEILAGLHLDSGSIKAALLHDVVEDTASTLPEI
                     QERFGDDVALLVDGVSKIDHLKFDSAAEAQAESFRKMLLAMAKDLRVILVKLADRTHN
                     MRTIASLPIEKQRRISRETLDIYAPIANRLGVYSIKLELEDLGFKTFHPYRYKVLERT
                     LRRAKGNQRQLLRKIEGKLGEALGDAKIPARVVAREKHLYSIYLKMQRKQAHLSDIVD
                     VYGVRVIVDDVDSCYRALGLTHQLFKPMPGRFKDYISIPRVNGYQSLHTTLFGPNGLP
                     LEVQIRTEGMDKVAERGIAAHWQYKAVDKDSHSTEERAREWLAGLMEMERAANSEEFL
                     ETVKADLFPDKVYVFTPKGAIMRLPRGATAVDFAYAVHTDVGNRCVAAKIDRRAVPLK
                     TQLQNGETVEIVTQRGAHPNPSWVNFVTTAKARNAIRSYLRSLKRDEAQELGSRLINQ
                     ELLSYSLSLKKVPRARLDQVVKELGLADQSALLEQVGLGERLAPVVAGLLAQEPDGGD
                     NADAPRKPLEIAGTEGSVVSYAHCCYPIPGDEIVGFMSSGRGIVIHRDRCRNLAEYRK
                     QPAKWIPVNWTRGVKAEFESEIQVRTLDRVGLLAEVAGRISAAHTNIEHVRVDRDGDA
                     SALAFRLKVRDRRQLAQVVRSIRTIPGVVRVSRSAG"
     gene            6794..7174
                     /locus_tag="VM692_06635"
     CDS             6794..7174
                     /locus_tag="VM692_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HUQ51881.1"
                     /translation="MTKEPVHTSKAPAAIGPYSQAIRAGSTVYLSGQIPLNPATMEIV
                     KGDIRAQIRQVFDNLAAVAEASGGSLANAVRLTVYLTDLANFPLVNEIMAQYCKEPFP
                     ARAAIGVAQLPRGAAVEVDGILVL"
     gene            7238..>8368
                     /locus_tag="VM692_06640"
     CDS             7238..>8368
                     /locus_tag="VM692_06640"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUQ51882.1"
                     /translation="MGPKAAERLAKLGVQTIGDLLCLLPQRYEDRTALRALGSLVVGE
                     KALVEGVIELSEVAVRRRRSLLCRLADGTGAITLRFFYFNRSQQQALARGQRVRCYGE
                     VRSGPAGLEMVHPEHRAVRADDAEPSDRLTPIYPTTEGLYQQSVRRLVGQALVVLERE
                     PLDDYLKPALESRALAGAPWPALADALRYLHSPPRDAATELLLSGRHPCSRRIALEEL
                     VAQRLSLRRSAASARSERARPLPVPAARLEAFREALPFALTRAQQSALDEIVVDLGAG
                     TPMNRLLQGDVGSGKTVVAAMAALVAAAAGCQTAVMAPTELLAEQHFASFDRWLAPFG
                     LEVVALLGGQPARTRAATLGAVADGRAAVVVGTHALFQDRVEF"
BASE COUNT         1447 a         2740 c         2768 g         1413 t
ORIGIN      
        1 cctgccccag cgtcgaacgg gtcgtaaact cattgccgca cgagcaaatc accttgatgg
       61 cggtgtaggt cggatggatc tctggcttca tggtcgggct ccgagcgggg cgcggaagaa
      121 taatgctcgt ccggctcgaa cacaaggcac gcggggcatc tcgtcgctgg cgccgcttat
      181 ccacacgagc tgtgtataac tctgtggaga tcgataggcg cccggcgatg aatcatcgat
      241 cagaaacgat ttcgccacat ttggttaaaa aacgatcagc gcggcgaact caacgttctc
      301 aacggcttaa agccatttcc ggccatcggt cgctaacaaa gcgccgcaac acgcttgttt
      361 cgatgcgacc gaacccggcg tgtgcataaa tcgcggctcg aaacgcgctg tgggcgcgcc
      421 aacttatcga ttcttcagga gttcgatcag ccggcgtagc gcggtgccac ggtgactaac
      481 gcgatttttt tcggccgctc cgagctgcgc cgcggtgcaa cgtaacgtgg gatcgaagaa
      541 aaccgggtcg tagccgaaac cgttgctgcc tcgcgctgct cgcgctatct cgccgtgcca
      601 ggagccggac gcgatcagcg gcgccgcatc gtccggcgac gtaagcgcga cgagcacgca
      661 gtgaaaatgc gcgcgacgct cgagcgcctg acccgccaac aacgtcaata gcttcgcaac
      721 gttcgcgccg tcgtcggcgt cttcgccggc aaaacgcgcc gagcgcacac cgggcgcacc
      781 gccgagcgcg tcgacgacaa gacccgagtc gtcggcaatg gcgggcagtc ccgtgacgcg
      841 tgcggcatgc cgcgccttgg cgagtgcgtt ctcgacgaac gtcacggcgg tctcgggcgc
      901 tggaatgaca ttgagctccg attgcgccgt cacgcgcacc gcgagccctg cctccgcgag
      961 caatgcgccg agctcggcga ccttgccgcg attgctggtt gcgagcaccc agtgcgcggg
     1021 tagcgtcact tcccgagcgc ggcggcctgc gccgcaagca agcgctcgat gccgactccg
     1081 gccaggtcga gaagcgtatt gagctcgctg cgctggaacg catgtccttc ggccgtccct
     1141 tgcacttcga tgaagccctt gccgccgttc atcacgacgt tcatatcggt ctccgcttcc
     1201 gagtcctcgg cgtagtcgag gtcgagcacg gcttgcccgc gaaaaatgcc gaccgacacg
     1261 gcggccacct gcccgtgtat gggatttcgc gagatcttgc cgttctttgc gagctgcgct
     1321 gccgcgagcg ccagcgcgac gtagccgccg gtgatcgcgg cagtccgcgt gccgccgtcg
     1381 gcttggagca cgtcacagtc gatcgtgatc gtgtgctcgc cgagcgcgtc gcgatcgacg
     1441 acggcgcgca agctgcggcc gatcagacgc tgaatctcga gcgttcggcc gctctgcgtg
     1501 ccgcgggcgg cctcgcgcgg agtgcggctg tgcgtcgcac gcggcagcat gccgtattcg
     1561 gccgtgaccc aaccctgcgc cttgcccttg aggaaaggcg gcaccttcgg ctcgacggaa
     1621 gccgtgcaaa gcacgcgcgt gtcgccgaac tcggcgagca cggatccttc agcgtgacgt
     1681 gtgaagtgcg gcgtcagctg cacgtggcgc atttcgtcgc ggcgccgtcc actcggccgc
     1741 atagcttgaa ctccgttcgt gacaaggccg cagcttacca gctcgcggca acgcgccgcc
     1801 gcgctagggg ccctgccagc gcaccgccgc gacgctcgtg ttgtcgctgt gcggcgagtt
     1861 gttcacgacg gcgcgatcgg ccaacgcgcg gatagcggtc tcgatcgagg tgccgggctc
     1921 gttcgccgcg gcgaggtcct cgtcctcgat ccccgaccac aacccgtcgg tgcaaacgag
     1981 caagacgtcg ttcggctcga gcttgttgcg cgccgtcacg ctcatgtcag gcagtggcac
     2041 gtcgccgccc aagcagcact cgacgaagtt gcgcatcggg tggttgcgca tctcgctctc
     2101 ggcgatcagc ccttcgcgca acagcaactc gacgtggctg tggtcgcgcg tgcgagcttg
     2161 gatatgcccg ccgcgcagtt gatagatgcg gctgtcgccg acgtgagccc agtacgcgcc
     2221 gccgtcttgc acgaggcaaa cggcacaggt cgctcgaggt ttgtgatcga gggctacgcc
     2281 gtcgcctagc ttgacgaccc ggtcgtgcgc ccgcgcgagc gacaacgtca gaaagccttg
     2341 cgggtcgaat atcggcagcg acatctcggc gaaggactcc ttgagcgtcg cgacggtcac
     2401 ctcggccgcg cgagccccat gggcgtggcc gcccatgccg tcaaccacca cgatcaacag
     2461 tgattcgccg ctggcaatga gatcgacacg atcctggttt tccgtccgat tccccggcag
     2521 ggtcagcttt gcataatcga cgcgcacgcc ttattaccgt cccctcgtct tcactcacgt
     2581 cttttcgtca ttgcgctcgc gcagccaagg ccgcagccgg accgaaaacc tgctgatgta
     2641 cactcggttc cgcagcgctg aacggcgctc agattggctg aaactacacc cggactcaat
     2701 cgaatgcctc acagcatgac tggattctcg accgcggagg cgcgcgtggg ggcgttcagg
     2761 ctcgtctggg agattcgcag cgtcaaccac cggtttctcg agctcgggtt tcgtctaccc
     2821 gaggatctgc gtgccgtcga gcccgagtgt agagagctgg tcggtacgac gatcaaacgc
     2881 ggcaaagtcg attgcacgtt gaaaatcagc gccgatgagc gcagcgcgac ggcggacacg
     2941 atcgtagccg gcgcactgga aaggctgcgc aagctcgagg agcaggtacg ccacgtgttt
     3001 cgcgacgcgc cgccgatgag cgcgcaagaa gtgctgcgtt ggccgggtgt cctcgaggaa
     3061 cctgcacaga atctcgagga gctcggtgaa gcggtcagag catgcttggg cgtcgcgctg
     3121 cgtgccctca cggacgcgcg ggcgcgcgaa ggcggtcgca tagcggagat gctcgaaaag
     3181 cgcaacgcgg gcatcacggc attgatcacc ggcatcaagc cgcagctcga cggcttgcag
     3241 ctgcgctacc gcgatcgcct acgcgagcgc ttgcagcggc tcgacgtgca agccgatccg
     3301 gaacgtttgg agcaagagtt ggcgctgatc gcgcagcgcg tcgatgtcgc cgaggagatc
     3361 gatcggctcg cgagccacgt cgccgaaatt caggcgattc tgcaccgcaa cgagccgatc
     3421 ggccgccggc tcgacttcgt cattcaagag ctcaatcgcg aagccaatac gtttggctcg
     3481 aaagtacaag aagaggcgct gacgcgcgtc gccgtcgagc tcaaggtgct gatcgagcaa
     3541 atgcgcgagc aggtgcagaa tctcgagtga gcgctactcc gcaacgcacg ggtcgacttt
     3601 gggtcgtcgc ggcgccatcg ggcgccggta agacgacgct cgtgcgagca ctgttggaac
     3661 gcgatccgct gctgcgcttc tccacgtcct acacgacgcg gcccccgcgc agctcggaag
     3721 tcaacggcaa ggactatttt ttcgtcgacg tgccgcagtt cgaggcgatg gtccgggacg
     3781 atgcgttcct cgagcacgcg caagtcttcg acaactggta tgggaccggc cgcgaccacg
     3841 tggcgagctt gctcgccgcc ggcttctcgg tagtgctcga gatcgattgg cagggtgcgc
     3901 agcaggttcg caagcgcgcg ccggactcgc aaagcatttt catcttgccg cccagcgtcg
     3961 gcgagctcga gcgacgcctc cgcggccgca agacggactc cgaggccgtg atccagaggc
     4021 ggctcaagga ttcgctcgcg gacatgggcc attggagcga gttcgaccat gtcatcgtca
     4081 acgaggactt tgccgcggct ctcgaccgat tggcggcggt agtcgcgggg cgtgagcacg
     4141 gagcgcggac gagcctaccc gcggtccgcg ctgcggtggc cgacatcgtg agcggtcgcc
     4201 cgaagacttg agcccttgct cgggccgggg ggtgctgcat tagacttacg ggtccaaagc
     4261 ccatggaggg ccgtccccaa tggcgagaat taccgtcgaa gactgtttgg agaaggttcc
     4321 gaacatgttc cagctcgtgc tcgtggccgc gaagcgcgcg cgccagcttg ccaacggcgc
     4381 ccatccgatg gtcgattggg agaacgacaa gccgacggtc gtcgccttgc gcgagatcgc
     4441 cgagggctac atcacggaag agatcctgac tcaacgcgac aagccggtcg aagacatcct
     4501 cgcggccgac ctggccgccg tcacgccgat gtcgccggac gagctgctcg gcacgaggcc
     4561 ggtttcactc ggcgactgaa gccattcacg gagggccggc cccgttgccg gcaacgagct
     4621 aagccatgga ttatggtgcc gccacactcg atcgaatcgt gtcgtcggtg tggcggacgg
     4681 acgactttgg ccggctcatg gggcagctcg agcgctactt gccgagtaac gacctcccga
     4741 gcatcacagc cgcttacgag ttcagcgcca aggcgcacca gggtcagcag cgccgcaacg
     4801 gcgaccccta tatctcgcac ccggtggccg tagcggaaat ccttgccggc ttgcatctgg
     4861 attcgggctc gatcaaagcc gcattgctgc atgacgtcgt ggaggacacg gcgagcacgt
     4921 tgcccgagat tcaagaacgc ttcggcgacg acgtcgcctt gctcgtcgac ggcgtcagca
     4981 agatcgacca tttgaagttc gacagcgccg cggaggcgca ggccgagagc ttccgcaaga
     5041 tgctgctggc gatggccaag gacttgcgcg tcatcctcgt gaagctggcc gaccgcacgc
     5101 acaacatgcg cacgatcgcc tctctgccga tcgagaagca gcggcgcatc tcgcgcgaga
     5161 cgctcgatat ctatgcgccg atcgccaatc gcctcggcgt ctactcgatc aagctcgagc
     5221 tcgaggacct cgggttcaag acgttccatc cgtatcgcta caaggtgctc gagcgcacgt
     5281 tgcggcgcgc gaagggtaac cagcggcagt tgctgcgcaa gatcgaaggt aagctcggcg
     5341 aagcgctcgg tgacgcgaag attcccgcgc gcgtcgtcgc gcgcgagaag catctctaca
     5401 gcatttatct gaagatgcag cgcaagcagg cgcacctctc cgacatcgtc gacgtctatg
     5461 gcgtccgcgt gatcgtcgac gacgtcgaca gctgctatcg cgcacttggg ctaacgcacc
     5521 agctgttcaa gccgatgccg ggccgcttca aggactacat ctcgattccg cgcgtcaacg
     5581 gctaccagtc gctgcacacg acgttgttcg gcccgaacgg cttgccgctc gaggtgcaga
     5641 tccgcacgga aggcatggac aaggtcgccg agcgcggcat cgccgcgcat tggcagtaca
     5701 aggccgtcga caaggactcg cacagcaccg aggagcgggc gcgcgagtgg ttggccgggc
     5761 tcatggagat ggagcgcgcc gcgaactccg aggaattcct cgagaccgtc aaggccgacc
     5821 tattcccgga caaggtgtac gtgttcacgc cgaagggcgc gatcatgcgc ctgccgcgcg
     5881 gcgcgacggc cgtcgacttc gcctacgccg tccacaccga cgtcggcaac cgctgcgtcg
     5941 cggccaagat cgatcggcgc gccgttccgc tgaagacgca gctgcaaaac ggcgagacgg
     6001 tcgagatcgt cacgcagcgc ggcgcgcacc cgaacccgag ctgggtcaac ttcgttacga
     6061 cggccaaggc gcgcaacgcg attcgcagct atctgcggtc gttgaagcgc gacgaggcgc
     6121 aggagctcgg cagccgcctc atcaaccaag agttgctgtc gtactcgctg agcctgaaga
     6181 aagtgccgcg cgcgcgcttg gatcaggtcg tcaaagagct tggtcttgcg gaccagagcg
     6241 cgctgctcga gcaagtcggc ttgggcgagc gcttggcacc cgtcgtggcc gggttgttgg
     6301 cacaggagcc cgacggcggg gacaacgccg acgcgccgcg caaaccgctc gagatcgccg
     6361 gcacggaagg gtccgtcgtc agctacgcgc actgttgcta tccgattccc ggcgacgaga
     6421 tcgtcggctt catgtcgagc ggccgcggca tcgtgattca ccgcgaccgc tgccgtaacc
     6481 tcgccgagta ccgcaagcag cccgccaagt ggattcccgt caactggacg cgcggcgtca
     6541 aagcggagtt cgaatcggag atccaggtcc gcacgctcga ccgcgtcggc ctgcttgccg
     6601 aagtggcggg ccggatctcg gccgcacaca cgaacatcga gcacgtgcga gtcgatcgcg
     6661 acggcgacgc gtcggcgctc gcgtttcgct tgaaagtgcg cgatcgtcgc cagctggcgc
     6721 aggtcgtgcg cagcattcgc acgattccgg gcgtcgtgcg cgtgtcccgc agcgccggct
     6781 gagctcagga agcatgacca aggaacccgt tcatacgtcc aaagcaccgg ccgcgatcgg
     6841 cccgtacagc caagcgatcc gcgccggcag caccgtgtac ctgtcggggc aaatcccgct
     6901 gaatcccgcg acgatggaga tcgtcaaagg cgacattcgc gcccagattc gccaggtatt
     6961 cgacaacctg gccgcggtag cggaagcgtc gggcggctcg ctggcgaatg ccgtgcgtct
     7021 gacggtctat ctgacggacc tcgccaactt tccgctcgtc aacgagatca tggcgcagta
     7081 ctgcaaggag ccatttcccg cgcgcgccgc gatcggagtc gcgcaattgc cgcgcggcgc
     7141 cgccgtcgag gtcgacggga tcctcgtttt gtaggcaacc tgaatcgctg cgcagcccga
     7201 atcgctgttc gatcgcccgg tccagcgcct gacgggcgtg ggccccaaag cggccgagcg
     7261 gctggccaag ctcggcgtgc agacgatcgg cgatctgctg tgcctgctgc cgcagcgata
     7321 cgaggaccgg accgcgttgc gcgcgctcgg cagcttggtc gtcggcgaga aggccctcgt
     7381 cgaaggtgtc atcgagctca gcgaagtcgc cgtgcgccgg cgtcgctcgt tgttgtgtcg
     7441 attggccgat ggcaccggcg cgattacgtt gcggttcttc tatttcaatc gctctcagca
     7501 gcaggcgttg gcgcgcggtc agcgcgttcg ctgttacggc gaagtgcgtt cgggcccggc
     7561 cgggctcgag atggtgcatc ccgagcatcg cgccgtgcgg gccgacgacg ccgagccgag
     7621 cgaccggctg actcccatct atccgacgac cgaggggctg tatcagcagt cggtccggcg
     7681 gctcgtcggg caagcgctgg tcgtgctcga gcgcgagccg ctcgacgact atctgaagcc
     7741 ggcgctcgag tcgcgggcgc tggccggcgc gccatggccg gcgctcgcgg atgcgctgcg
     7801 ctatctacac tcgccgccgc gcgacgcggc gacggagctg ctgctctcgg gacgccaccc
     7861 ctgctcgcgg cgcatcgcgc tcgaggaact ggttgcgcag cgcttgagct tgcggcgttc
     7921 ggcagcgagc gcccgttccg agcgtgcgcg gccgctcccc gtgcccgcgg cgcggctcga
     7981 ggcgtttcgc gaggcgttgc cgttcgcgct gacgcgcgcg caacagagcg cgctcgacga
     8041 gatcgtcgtc gatctcggcg ccggcacacc gatgaatcgt ttgctgcagg gcgatgtcgg
     8101 ctctggcaag accgtcgtcg ccgcgatggc agcgctcgtc gcggcggccg cgggctgtca
     8161 gacggccgtc atggcgccga cggagctgct cgcggagcag cacttcgcga gctttgaccg
     8221 ctggctcgcc ccgtttgggc tcgaggtcgt ggcgttgctc ggcggccagc ctgcgcgcac
     8281 gcgtgctgcg acgctcggcg ccgtcgcaga cggccgcgcg gccgtcgtgg tgggcacgca
     8341 cgcgctgttc caggacagag tcgagttc
//
LOCUS       DATLEE010000312         9887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9870411, whole genome shotgun sequence.
ACCESSION   DATLEE010000312 DATLEE010000000
VERSION     DATLEE010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9870411"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VM692_06645"
     CDS             <1..257
                     /locus_tag="VM692_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HUQ51883.1"
                     /translation="QYVESVEVINGRLDLRFGYRADHAISGRTLSLTPFETATHEVVW
                     VCGNARPGAGLEPLGFSLGGQQAVQADTLIEPRYLPSTCR"
     gene            469..2196
                     /gene="pilB"
                     /locus_tag="VM692_06650"
     CDS             469..2196
                     /gene="pilB"
                     /locus_tag="VM692_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HUQ51884.1"
                     /translation="MAVTATQGPLTGLPRRLVQDGVVSEADLMAALNAIEGKSANLVP
                     HLVANRLGDPRQIAIAAAHEFGVPLLDLDAVDLDLDVVKLVDEKLLLKHRILPILQRG
                     KRLFLAVCDPTNLQALDEIKFQTTLRIEAIVVEQDKLDARVGRAIEAVDTTMSSLDDD
                     GEGFDLENLEVSGGEEDPGAEVTTSDIDDAPIVRFVNKVMVDAIKKGASDIHFEPYEK
                     HFRIRLRQDGVLTEIARPPVALAMKVSARLKVMARLDIAERRIPQDGRIKLKLSKNRA
                     IDFRVNTCPTLFGEKVVCRLLDPSSAQLGIDALGYEEEQKQHYLKTLAKPYGMILVTG
                     PTGSGKTVSLYTGLNILNTVDRNISTAEDPAEINMPGVNQVNVNPKVGLTFAAALRAF
                     LRQDPDIIMVGEIRDLETAEIAIKAAQTGHLVLSTLHTNDAPKTLTRLVDMGVKPYAI
                     ASSVSLIIAQRLARRLCSNCKEAVDIPAEALLKEGFTKADIESGMTIFSAKGCNQCTN
                     GYKGRVGIYQVMPCSEHIGRIMMEGGNSMQIAVAAKAEGVADLRQSGLKKVKDGLTSL
                     EEINRVTVD"
     gene            2246..3466
                     /locus_tag="VM692_06655"
     CDS             2246..3466
                     /locus_tag="VM692_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUQ51885.1"
                     /translation="MATATATATQAPFTWEGTDRTGKKVKGKVVSTSEAAVRTELRRQ
                     GVVPTRVRKQSMLFRKQGKVTAADIAIFSRQLATMMTAGIPLVQSFDIVGAGHENPAM
                     QKLILAIKSDVEGGTALADALGKHPLHFDDLFVSLVAAGEQAGALETLLDKIATYKEK
                     TEAIKKKIKKALFYPAAVVVVAIVVTVILMIYVIPEFESLFQGFGADLPAFTRFVINI
                     SKFVQSTGIFLLIGAVGAVWGFLYAKKRNRGVQHFVDRVMLKLPIIGSILHKSAIARY
                     ARTLSTTFAAGVPLVEALDSVAGATGNIVYEGAVMKMRDEVATGQRLQRAMENTDLFP
                     NMVNQMIAVGEESGSLDAMSAKVADFYEEEVDNAVDSMSSLLEPLIMAVLGVLVGGLV
                     IAMYLPFFKMGAVV"
     gene            3490..4377
                     /locus_tag="VM692_06660"
     CDS             3490..4377
                     /locus_tag="VM692_06660"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HUQ51886.1"
                     /translation="MEIFEAWPITLFIVAGLLGLVVGSFLNVVAYRIPIMMERAWREQ
                     CAELKSQPLTPPSHIADGSRFDLWWPPSACPACGGAIAAQHNVPVFGFLWLKGRCARC
                     GAKISARYPVVESLAGMLAVTVAYVFGPSWQTVAALGFAWTLLALTLIDLDHKLLPDS
                     LTLPLLWAGLLVNVPVAGDGLFAPLRSSVIGAAFGYLALWSVYKLFKLVTGKEGMGYG
                     DFKLLAAIGAWLGWQLLPVVILLSAVVGSVVGIALIAFGGRSSQTAIPFGPYLAAAGL
                     IAMLWGERLIQLYQAWFFR"
     gene            4435..5067
                     /gene="coaE"
                     /locus_tag="VM692_06665"
     CDS             4435..5067
                     /gene="coaE"
                     /locus_tag="VM692_06665"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279997.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HUQ51887.1"
                     /translation="MTDNAIAGPKLIVGLTGGIASGKSLVGAMFVKLGASLIDTDVVA
                     REVVARGEPGLAAVSAEFGPKVLLPSGELNRPALRSLVFADDAKRRQLEAILHPLIRS
                     RTRARLAELEAPYALVAVPLLVETNFGELVDRILVVDAPEPAQLERLMRRDAIPRAEA
                     LEMLRAQVDRATRLKVAHDVIDNSGTSDATRRQVESLHRRYLDLAAQRAR"
     gene            5111..5911
                     /gene="zapD"
                     /locus_tag="VM692_06670"
     CDS             5111..5911
                     /gene="zapD"
                     /locus_tag="VM692_06670"
                     /inference="COORDINATES: protein motif:HMM:NF003656.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HUQ51888.1"
                     /translation="MNTAAELPSASAEHALISFEQPLSERMRTFLRVEFLYEQTLFHV
                     DEPTEFSARAAVAALLEILTILGRGDVRTDVNKELERHAELLGRYRSQPGVDPARLTG
                     LIDNIDELKQQLSEAGPQVVNPLKECDFLTTIRHRSAIPGGTCMFDLPDYGYWLHLPA
                     AERRRQLDDWTNCIKPICDAVAEVLWLTREATEAAPQVAPGGLFQHGFDRSDQASLVR
                     VLLPANGGIFPEISAGQHRFTIRFVRWRGVDARPAQVNQDVRFQLAIC"
     gene            5957..6373
                     /locus_tag="VM692_06675"
     CDS             5957..6373
                     /locus_tag="VM692_06675"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HUQ51889.1"
                     /translation="MQRTGLGRFASFVAVGGLGFVVDAAILSTLVYVWDWPHYSARVI
                     SFAAAVSVTWYCNREWVFTRTKDRAREYGAYFGVQAVGAVINLGTYAVLIALVPSLAR
                     LPLVPLAAGAALALLFNYSGATRWVFADPARKDRSR"
     gene            6370..7080
                     /locus_tag="VM692_06680"
     CDS             6370..7080
                     /locus_tag="VM692_06680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ51890.1"
                     /translation="MTNASDSPSPYTGVENLEVMREAENYNRYLLALVQRHAHGATRM
                     VDFGAGSGTFAVPCAAAGFDVTAVEPDEPLRAMLAAAGVRAVADAGALPDAVAPYVYT
                     LNVLEHIPDDVGALRTLRAKLVPGGRLFVYVPAFPVLYTSMDAKVRHVRRYTRATLSA
                     SLVAAGLEIETMRYADSLGFAATLLFKALDNGSGDVNRTMLRLYDRLAFPVSRAFDVL
                     ADRWFGKNLLALARKPAA"
     gene            complement(7077..7499)
                     /locus_tag="VM692_06685"
     CDS             complement(7077..7499)
                     /locus_tag="VM692_06685"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HUQ51891.1"
                     /translation="MSGQRVVDVVVGLISDGAGHWLVNCRPAGTPLAGSWEFPGGKLN
                     TGEAPFAALKRELDEELGIDVLEARPLLTLTHDYPDKRVRLDVWHVLRYRGNASAREG
                     QPLKWVTVEECRALELLEADWPIVAKLPLLEGGASHGG"
     gene            complement(7582..9843)
                     /gene="secA"
                     /locus_tag="VM692_06690"
                     /pseudo
     CDS             complement(7582..9843)
                     /gene="secA"
                     /locus_tag="VM692_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758483.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
BASE COUNT         1654 a         3189 c         3160 g         1884 t
ORIGIN      
        1 ggcaatatgt cgaatcggtc gaggtcatca acgggcgcct ggacctgcgg ttcggctacc
       61 gtgcggacca tgcgatctcg gggcgcactc tgtcgctgac gccgttcgag actgcgacgc
      121 atgaagtcgt ctgggtctgc ggcaacgcac ggccgggcgc gggcctagag ccgctcgggt
      181 tctcactcgg cgggcagcaa gccgtgcagg ccgacacgct gatcgagccc cgctaccttc
      241 catcgacgtg ccgctgaacg tccgagccgt ggcaggcacc ccggccagca caattttgca
      301 ggcctcatca ccgcggtcga cgcgcatcgg ggtgcacaag cctgcctctg tgcagcgacg
      361 gaatctcggt cacaaaacca gatctcgtcg attgacagcc catcccggca cggcgagagt
      421 acagcgttta ttgtcaaagg cggccagtta cctctctcgg gcggattgat ggcggttact
      481 gcaactcaag gtccgttgac cggcctgccg cgtaggctgg tgcaggacgg cgtcgtctcg
      541 gaggccgatc tgatggcggc cctgaacgcg atcgagggca agagcgcgaa cctagtaccg
      601 cacctggtcg ccaatcggct cggagacccc cgtcagatcg cgatcgcggc ggctcacgag
      661 ttcggcgttc ccctgctcga cctcgacgcg gtcgacctcg atctggacgt cgtcaagctc
      721 gtcgacgaga agctgctgct gaagcaccgg atcctgccga tcttgcagcg cggcaagcgg
      781 ctcttcctcg cggtctgcga cccgacgaat ctccaagccc tagacgaaat caagttccag
      841 accacgctgc gcatcgaagc gatcgtcgtc gagcaggaca agctcgacgc gcgcgtcggc
      901 cgtgcgatcg aagccgtcga caccacgatg tcgagcctcg acgacgacgg cgaagggttc
      961 gatctcgaga acctcgaagt cagcggcggc gaggaagatc ccggcgccga ggtgacgact
     1021 tcggacatcg acgacgcgcc gatcgtccgg ttcgtcaaca aggtcatggt cgacgccatc
     1081 aagaagggtg catcggatat ccacttcgag ccttacgaaa agcatttccg catccgcttg
     1141 cgccaagacg gcgtgttgac cgagatcgct cggccgcctg tggctctggc aatgaaggtt
     1201 tccgcgcgtt tgaaagtcat ggctcggctc gacatcgccg agcggcgcat tccgcaagac
     1261 ggccgcatca aattgaagct gtcgaagaac cgcgccatcg acttccgggt caatacgtgc
     1321 ccgactttgt tcggcgagaa agttgtttgc cggttgctcg acccgtcgag cgctcagcta
     1381 ggcatcgacg cgctcggcta cgaggaagaa cagaagcagc actatctcaa gacgctcgcg
     1441 aagccctacg gcatgattct cgtgacgggc cccacgggta gcggcaagac ggtgtcgctg
     1501 tacaccggcc tcaatatttt gaacaccgtc gaccgcaaca tctcgacggc cgaagacccg
     1561 gccgaaatca acatgcccgg cgtcaatcag gtcaacgtca atccgaaggt tggcctcacg
     1621 ttcgcggcag ctttgcgtgc gttcctgcgc caagacccgg acatcatcat ggtcggcgag
     1681 atccgcgacc tcgagaccgc tgagatcgcc atcaaggcgg cacagacggg acacttggtg
     1741 ttgtccacgc tgcacacgaa cgatgcaccg aagacgttga ctcgtctcgt cgacatgggt
     1801 gtgaagccct acgccattgc gagctcggtc agtttgatca tcgctcagcg gcttgcgcgg
     1861 cgcttgtgca gtaactgcaa ggaggccgtc gacatccccg ccgaggcctt gctcaaggaa
     1921 ggtttcacga aggccgacat cgaaagcggc atgacgattt tcagcgccaa gggttgcaac
     1981 cagtgcacga acggctacaa ggggcgcgtc ggcatatacc aggtaatgcc gtgctccgag
     2041 cacatcggcc gcatcatgat ggaaggcggc aactcgatgc agattgccgt ggcggccaag
     2101 gccgagggcg tcgcagacct gcggcaatcc ggcctcaaga aagtcaagga cggccttacc
     2161 agtctcgaag agatcaaccg ggttacagtt gactagactc tagcggccga cggacgggcc
     2221 gctgataccc gttggagagg atttgatggc taccgcaacc gccaccgcga cccaagctcc
     2281 cttcacgtgg gaaggcacgg accgcaccgg caagaaggtc aagggcaagg tcgtctcgac
     2341 gagcgaggcg gcagtccgca ccgagctgcg gcgccagggc gtcgtcccga cgcgcgtgcg
     2401 caagcagagc atgctgtttc gcaagcaggg caaggtcacg gcagcggata tcgcgatctt
     2461 ttcgcgccag cttgcgacga tgatgacggc gggcattccg ctcgtgcagt cgttcgatat
     2521 cgtcggcgcg gggcacgaga accccgcgat gcagaagctg attctcgcga tcaagtccga
     2581 cgtcgagggc ggcacggcgc tggccgacgc gctcgggaag catccgctgc acttcgacga
     2641 cctgttcgtg agccttgtgg cggccggcga gcaagccggt gcgctcgaga cgttgctcga
     2701 caagatcgcg acgtacaagg aaaagaccga ggccatcaag aagaagatca agaaggcgtt
     2761 gttctatccg gcagcggtcg tcgtggtcgc aatcgtcgtc accgtgatcc tgatgatcta
     2821 tgtcatcccg gagttcgagt cgctgttcca ggggttcggc gcggacctgc ccgcgttcac
     2881 gcgcttcgtc atcaacatct cgaaattcgt gcagtcgacg ggcatctttc tactgatcgg
     2941 tgcggtgggc gcagtttggg gatttctcta cgcgaagaag cgtaatcgcg gcgtgcagca
     3001 tttcgttgac cgcgtgatgc tgaagctgcc gatcatcggc tcgatcctgc acaagtccgc
     3061 aatcgcgcgt tacgcgcgaa cgctgtcgac gacgttcgcg gccggcgtgc cgctcgtcga
     3121 ggcgctcgac tccgtggccg gcgccacggg caacatcgtc tatgagggcg ccgtgatgaa
     3181 aatgcgcgac gaggtcgcga cgggccagcg gttgcagcgc gcaatggaga acaccgacct
     3241 tttcccgaac atggtcaatc agatgattgc ggtcggtgag gagtcgggct ccttggacgc
     3301 catgtccgcc aaggtcgccg atttctacga agaggaagtc gacaacgccg tcgacagcat
     3361 gtcgagcttg ctcgagccgc tgatcatggc ggttctcggc gtgctcgtcg gtggtctcgt
     3421 catcgctatg tacttgccgt tcttcaagat gggcgccgtc gtctgacgcc gctcgttcgc
     3481 ggctgacgcg tggagatttt cgaggcctgg ccgattacgc tgttcatcgt cgcggggctc
     3541 ctgggtctcg tcgtcggcag cttcttgaac gtcgtcgcct atcgcatccc gatcatgatg
     3601 gagcgcgcct ggcgcgagca gtgcgccgag ctgaagtcgc aacctttgac gccgccctcg
     3661 cacatcgccg acggctcgcg cttcgatctc tggtggccgc cgtcggcctg tcctgcctgc
     3721 ggcggggcga ttgccgcgca gcacaacgtg ccggtcttcg gcttcctgtg gctgaaaggg
     3781 cgttgtgcgc gctgcggcgc gaagatctcg gcgcgttacc ccgtcgtcga atctctcgcg
     3841 ggcatgcttg ccgtcaccgt cgcctacgta ttcgggccga gctggcaaac cgtggccgct
     3901 ttgggcttcg catggacgtt gttggcactg acgctgatcg atctcgacca caagctgctg
     3961 cccgattcgt tgaccctgcc gctcctttgg gccggcctgc tcgtgaacgt gcccgtcgcc
     4021 ggcgacggat tgttcgcgcc cttgcgctcg agcgtcatcg gcgcagcgtt cggctatctc
     4081 gcgctatgga gcgtctacaa gctctttaag ctcgtcacgg gtaaagaagg catgggctat
     4141 ggcgacttca agctcctcgc agcgatcggc gcctggctcg gttggcaact cctgcccgtc
     4201 gtaattctgt tatcggcggt cgtcggcagc gtcgtcggca tcgcgctgat cgccttcggc
     4261 ggccggtcga gccaaaccgc cataccgttc gggccttacc tcgccgccgc aggtctcatc
     4321 gccatgctct ggggcgagcg gctgattcag ctctaccaag cgtggttttt cagatagggt
     4381 acgcaccacc gaacctaacg cccaactggg cagtcgcgaa cagccaaggg tgcgatgacc
     4441 gacaacgcaa tcgctgggcc gaagctcatc gtcggcctga ccggcggcat tgcgagcggc
     4501 aagtcgctcg tcggagcgat gttcgtgaag cttggagcgt cgctgatcga caccgacgtc
     4561 gtcgctcgcg aggtcgtggc acgcggcgag ccaggtttgg ccgccgtcag cgccgaattc
     4621 gggccgaagg tgctgctgcc ttccggcgag ctcaatcgcc ccgcgctgcg ctcgctcgtg
     4681 tttgccgatg acgccaaacg gcgacagctc gaagcaatac tgcacccgct gatccgcagt
     4741 cggacccgcg cgaggctcgc cgagctcgag gctccctacg cgctcgtggc cgtgccgcta
     4801 ttggtcgaga caaatttcgg cgagctcgtg gatcggattc tcgtcgtcga cgcgccggag
     4861 ccggcgcagc tcgagcgctt gatgcgccgc gacgcgattc cgcgcgcgga ggcgttggaa
     4921 atgctgcgcg cgcaggtcga tcgcgcgacg cgcttaaaag ttgcgcacga cgtcatcgac
     4981 aacagcggca catcggacgc aacgcggcga caagtcgagt cgctgcatcg acgctatctc
     5041 gatctcgcag cacaacgcgc tcgctgaaag ttcgtttgcc gtgcgtggtg cgctcgcgca
     5101 gaatagcggc atgaacaccg ccgctgagct cccgtccgca tcggccgagc acgcactgat
     5161 ttcgttcgag cagccgctca gcgagcggat gcgcacgttc ctgcgcgtgg agttcctata
     5221 cgagcaaacg ttgttccacg tcgacgagcc gaccgagttc agcgctcgtg ccgccgtcgc
     5281 ggcgctgctc gagattctca cgatcttggg tcgcggcgac gttcgcacgg acgtcaacaa
     5341 agagctcgag cgccacgccg agctgctcgg ccgttaccgc agtcagcccg gcgtggatcc
     5401 ggcccggctc acgggcctta tcgacaacat cgacgagctc aagcagcagc tctcagaggc
     5461 cggcccgcaa gtcgtcaacc ctctgaaaga gtgtgacttt ttgaccacca tccgccaccg
     5521 aagcgcgatc cccggcggca cctgcatgtt cgacctgccc gattacggct actggctgca
     5581 cttgccggcg gccgagcgcc gacggcagct cgacgattgg accaactgca tcaagccgat
     5641 ctgcgacgcc gtggccgaag tcctgtggct cacgcgggaa gcgacggagg ctgctccgca
     5701 agtcgcgccc ggcgggctgt tccaacacgg cttcgaccgc agcgatcagg caagcctcgt
     5761 gcgcgtcctg ctgccggcca acggcggcat ctttcccgaa atcagcgcgg gccagcaccg
     5821 cttcacgatt cgcttcgtgc gctggcgcgg cgtcgacgca cggcctgcgc aggttaatca
     5881 agacgtacga ttccagcttg cgatctgctg agcgggcgtg aagccgcgcg agtccctcga
     5941 ggtcggggcg tccacgatgc aacgcacggg gctcgggcga ttcgcatcgt tcgttgcggt
     6001 cggcggtctt ggctttgtcg tcgatgcggc catcttgtcg acgctcgtct atgtttggga
     6061 ctggccgcac tacagcgcgc gagtgatctc gttcgccgct gcggtctccg tcacttggta
     6121 ctgcaaccgc gagtgggtgt tcacgcgtac gaaggaccgt gcacgtgaat acggcgcgta
     6181 ttttggcgtt caagccgtcg gcgcggtcat caacctcggc acgtacgccg tcctgatcgc
     6241 gctcgttccg tcgctcgcgc ggttgccgct agtgccgttg gcagccggcg cagcgttggc
     6301 gttgttgttc aactattcgg gcgcaacccg ctgggtattc gctgaccccg cccgcaaaga
     6361 ccggagccga tgacgaacgc ctcggattcg ccctcgccgt ataccggtgt cgagaacctc
     6421 gaggtcatgc gcgaggcgga aaattacaac cgctatctac tcgcgctggt gcaacgtcac
     6481 gcgcacggcg ctacccgcat ggtcgacttc ggcgccggaa gcggaacatt cgcggtgccc
     6541 tgcgcagcgg ccggcttcga tgtcaccgcc gtcgagcccg acgagccgct gcgcgcgatg
     6601 ctggcagcag caggggtgcg cgcggtcgca gatgctggag cgctaccgga cgccgtcgcc
     6661 ccttacgtct acacgttgaa cgtgctcgag cacatcccgg acgacgtggg cgcgcttcgc
     6721 acgctgcgcg cgaaactcgt gccgggcggg cggttgttcg tctacgtccc tgcatttccc
     6781 gtgctttaca cgagcatgga cgccaaggtg cggcacgtgc ggcgctatac gcgcgctact
     6841 ttgtcggcca gcctcgttgc agcggggctc gagatagaga cgatgcgcta cgctgactcg
     6901 ctgggcttcg cggcgacatt gctgttcaaa gctttggaca acggcagcgg ggacgtcaat
     6961 cgaaccatgc tgcggcttta cgatcgattg gcgttccccg ttagccgggc gttcgatgtg
     7021 ttggcggacc gctggttcgg caagaatctg ctagcgctcg cccgaaagcc cgccgcctaa
     7081 ccgccatgcg acgcgccgcc ctcgagcagc ggcagcttcg cgacgatcgg ccaatcggcc
     7141 tcgagcaact cgagcgcgcg gcactcttct accgtgaccc acttgagcgg ctgtccttcg
     7201 cgcgcgctcg cgttgccgcg ataacgcagc acgtgccaga cgtcgaggcg gacgcgcttg
     7261 tcgggatagt cgtgagtcag cgtcagaagc ggccgcgctt cgagcacgtc gatgccgagc
     7321 tcctcgtcga gctcgcgctt gagtgcggcg aacggggctt cacccgtgtt gagcttgccc
     7381 ccggggaact cccacgagcc ggcgagcggc gtgccggccg ggcgacaatt cacgagccaa
     7441 tgccccgcgc cgtcgctgat caagccgacg acgacgtcga cgacgcgctg cccgctcatg
     7501 acccttggaa atccccgacg ggggacgccg cttcgtagtc cccgacgggg gacgccgctt
     7561 cgtagtcccc gacaggcgtg ctcaactcaa tcggccgtgg cactgcttga atttcttgcc
     7621 cgagccgcag gggcacggat cgttgcgccc caccttcggt gcttcgcgca cgtacggcgt
     7681 gaccggctcg gccgccgccg gcggcggccg caggccggaa cctggcgcag gcgcgctcgg
     7741 ccgcggctcc ggctccggcg cggccggaac gagcggcggc gccgccgcgt gctggaactt
     7801 cagagtgctc gggtccgggg gcgcgggctc gacggcttgc acgtcctccg gccgccggat
     7861 ttggatcttc gagagaatgc tgatcgtgtc gtgcttcacg cgctcgagca tggagctgaa
     7921 catctcgaac gcttcacgct tgtactcctg cttcggattg cgctgcgcat agccacgcag
     7981 gtggatgccc tgacgtaggt aatcgagcgc gccgatgtgc tccttccagt gcgaatcgag
     8041 ctgacgcagc attatctgct tctcgatctc gtgcatgacc ttcgggccga tctcggcgac
     8101 cttgcggtca taggcttccg caaccgcggc gagggcgagc tcgcgcaacc gtttctcttc
     8161 gatcgtgtgg tccgcctcga gcatgccgac cacgtccaag tggacgccga actcgcgctc
     8221 cagggctttg ctcaagccgt ctttgtccca ttcttcctcg acgctttccg gcgctacgtg
     8281 ctcgttgatc gcggcattga cgacttcggc acggatgttg cgaatcgcgt ccgaaatgtc
     8341 gctcgcttcc atgagctccg agcgctgttg atagacgact cgccgttgat cgttggcgac
     8401 gtcatcgtat tcgagcagct gcttgcggat atcgaagttg tgcgcctcga ccttgcgctg
     8461 cgctcgctcg atctggcgcg acagcagctt gctttcgatc gcctcgccgg cctacatacc
     8521 ggcgcgcgac agcatgcgct tgatgcgctc cgggtcgccg aagatgcgca tgagattgtc
     8581 ttcgagcgac aggtagaagc gactcgagcc ggggtcgccc tggcggccgg aacggccgcg
     8641 cagctgattg tcgatgcgcc gcgattcgtg ccgctcggtg ccgacgatgt gcagtccacc
     8701 catctcgagc accttcgagt ggcgcgcctt ccagtccgct tcgagcgcct cgagctcggc
     8761 ctggcggcga accgcgcgtt cggcgtcgcc cgccgcgcca tgatcggcgt cgtcggaagg
     8821 caatgtggcg cgctcggcct cgagattgcc gccgagcttg atgtcggtgc cgcggccggc
     8881 catgttggtc gcgatcgtca cggtgccggg acggccggcc tgcatgatga tctcggcctc
     8941 gcgctcatgg aatttggcgt tcaagacctg gtgcggaatc ttttgcttct tgagcaggcc
     9001 cgacaggaat tccgacgtct cgatcgaggt cgtgccgact aacacgggtt ggccgcgcgt
     9061 gttgcagtcc ttgatgtctt cgatgatcgc gtcgaacttg tccttctgcg tcagatagac
     9121 gagatccggc gcgtcgtcgc ggatcatcgg catgtgcgtc gggatcacga cgacctccag
     9181 gccgtagatc tgctggaact cgtaggcttc cgtgtccgcc gtgcccgtca tgccggcgag
     9241 cttcttgtac atgcggaagt agttctggaa cgtgatcgag gcgaccgtct ggttctcctc
     9301 tttcacgcga acgccttcct tggcctccac agcttggtgc aagccgtcgg accagcggcg
     9361 gccgaccatg gttcggcccg tgaattcgtc gacgatgatg acctcgccgt ccttcacgat
     9421 gtaatcgacc tcgcgcttga acagcccgtg cgcgcgcaac gccgccgtca ggtgatgcat
     9481 gaggcggatg ttcgcggggt cgtacaggct ctcgccttcc gtcaagaggc cggccttcag
     9541 cagcaggcgc tcgacgtact cgtggccttc ctcggtcaga tgcgcttgct tcgacttttc
     9601 gtcgatggcg tagtgacccg ggacgaacag ctgcgctttt gcaggcttgg attcgttgtc
     9661 gacctcgatg aggtggtggc cgccctcttt tgctttcgcg agggccgcgc ccgtcggcat
     9721 ctcgccgagc gagttgccgg tgacaccgag cagctcgacg tgcgtcgact cgatttcgtc
     9781 gttcgccttg cccggcggcg gcttatgcac cttcagcgac gggatcagct gattaatccg
     9841 cacgtagagc tcggtgctct cctcggagcg gccggaaatg atcagcg
//
LOCUS       DATLEE010000313         5275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4068829, whole genome shotgun sequence.
ACCESSION   DATLEE010000313 DATLEE010000000
VERSION     DATLEE010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4068829"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VM692_06695"
     CDS             complement(<1..232)
                     /locus_tag="VM692_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51892.1"
                     /translation="MDDTVEIIAVVLGDASERRWRAGQLGRGAQCLVRRSDGRVALEP
                     VASEEEFERTARTTGCFRRGKPIHDPFTHEVIG"
     gene            459..851
                     /locus_tag="VM692_06700"
     CDS             459..851
                     /locus_tag="VM692_06700"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HUQ51893.1"
                     /translation="MLKLKRRIQDGTNGPTTYVAASTKLVGTISGQGTYVFCGTVEGD
                     CDINGPLTLAEGGRWKGTIRATDIVVAGTVEGDVVAQQRVEILGSARVTGSLAGNSIA
                     VAEGAIIEGEIKVASGASPTTFQEKRQA"
     gene            complement(859..1842)
                     /locus_tag="VM692_06705"
     CDS             complement(859..1842)
                     /locus_tag="VM692_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUQ51894.1"
                     /translation="MPHAAQAFDPPVGLRHRHVQSLLAGWPWRQRWLRRRAARVLAAS
                     RDEIVDCGDGVRLLGHYSAQPGAAKGLAILLHGWEGSAAASYVISAGSLLFEAGYSVF
                     RLNFRDHGETFELNRDLFHSCRIDEVVGAVGEIAVRHPADRTFIVGHSLGGNFALRVA
                     ARAPAAHIPVTKVVAICPVLRPTSTMRALEEGLWVYRQYFLRRWRRSLLAKAACFPEL
                     YDFGDLRRFATLTTTTEFFATRYARFPDLESYLNGYAITGDALARLEVPARLIAAADD
                     PVIPIADLADVAVTPALKIDVVPRGGHCAFLEDYGLRSWLDRAVLAELEAS"
     gene            1962..4016
                     /locus_tag="VM692_06710"
     CDS             1962..4016
                     /locus_tag="VM692_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ51895.1"
                     /translation="MLATFINSPLKRRFMAAVLLLAGSVLLALGGLVYLYVAGNLSLA
                     LFGGLAFICIAAATVVGLYLVRTIIDRFSAPLRTVTASLQSANGELDAARFADTTELR
                     ELAAAVNAMRTNLRSSTISRDYLDRLLSSMGEALLITNAEGKVERANGAAVELFGRTE
                     EALIGKLADDLILSNDRRRAEGAASRPREGTVLRPDGTTVSVSYTVANVHDGDALQSK
                     VYAAHNIDERKRVEQRIRYLARTDSLTKIANRMQFQHLLQQAIARARRTQQYVAIVYL
                     DVDRFKDINDTFGHAAGDTSLEIFARRVLAELGESSHAGRLAGDEFAVLITGFERLDA
                     MQRHLNELAPRLLTATGKPFQVHGEEIFLTASMGIAVYPRDGDNVIDLIRNADAALYE
                     AKKAGGNCFEYYSSDMNTEAVERLMLKSKLRRAFERDELRLHYQPKYRISTGRIEGVE
                     ALLRWDLPERGLVFPSDFVPLAEETNLILQLGDWVLNRVCADYRAWQRSVPSPCRVSL
                     NLSLRQLQQRRFLDQVRDTFRAHGVSPTSLELEITETTLMEDPERTVRILDALYGMGL
                     HLAIDDFGTGYSSLSALQQFPINTLKIDQSFVRDVAIDRDDAAIVDAIIQMAHSLRLE
                     VVAEGVESEAQLEFLRSHGCDHAQGHLFGDPVTADQFCELLLAESAGTGKYRALFAGA
                     GV"
     gene            4081..4431
                     /locus_tag="VM692_06715"
     CDS             4081..4431
                     /locus_tag="VM692_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51896.1"
                     /translation="MKVLTSGLVAAIATGYGVAAGACENPTAVAIPDGKSATMEQMLA
                     AQAQVKAYQTAMNAFLACLDTELDAQGEQAPEDFKSLMVSRHNAAVSEMEGVAAAFND
                     QIKAYRAANPAPPK"
     gene            complement(4485..4712)
                     /locus_tag="VM692_06720"
     CDS             complement(4485..4712)
                     /locus_tag="VM692_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51897.1"
                     /translation="MNPFEFALAMVALVLIFKLIRTAMAHKNAQPPRDDGVNTDLLER
                     LHQVEERVRVLERIVTDERYELKQQFKDLGA"
     gene            complement(4756..5175)
                     /gene="pspC"
                     /locus_tag="VM692_06725"
     CDS             complement(4756..5175)
                     /gene="pspC"
                     /locus_tag="VM692_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HUQ51898.1"
                     /translation="MNESPREPGLINKLYRLPRRGMIFGVCAGIAEYFGFDVTVTRVI
                     VAATAFFAFPVICVVYLLLGLLLPPRRYSDGDRDYNDPAEREERSIRRQVRSSPHDTL
                     ASVRYRFRDLDVRLQRLEKYVTSNRYKLDREFEQLGK"
BASE COUNT         1018 a         1703 c         1654 g          900 t
ORIGIN      
        1 aaccgatgac ttcgtgcgtg aaagggtcgt gaatcggctt gccgcgacga aaacagcccg
       61 tcgtccgagc cgtgcgctcg aactcctctt ccgatgcgac aggctcgagc gcgactcgac
      121 cgtcggaccg acgcaccaag cattgcgccc cgcgtccaag ctgacccgcg cgccagcgtc
      181 gctcactcgc atcgccgagc acgaccgcaa tgatctctac cgtatcgtcc atcagcgagc
      241 ctcgagccca agtgctagtt ccaaatagga agcaatctgc gtgccaagga aacgccgtca
      301 aggccaaaca acctcttacg gcgcgggtct gtgagcgctt tcacaccagc gatgcgacaa
      361 aatttggcac tctgcggcgg tttgttgcca cgtgacctgg ttcccgcgga accggctcac
      421 gttcgagtac cttgcctcac ggctcgcgga gacacgaaat gctgaagctg aaacgacgaa
      481 tccaggatgg cacgaacggc ccgacgacct acgtcgccgc ctcgaccaag ctcgtcggca
      541 cgatttcggg tcaagggacc tatgttttct gcggcaccgt cgagggcgac tgcgacatca
      601 acgggccgct gacgctcgcc gaaggcggcc ggtggaaggg cacgatccgc gcgaccgaca
      661 tcgtcgtggc gggaacggtc gaaggcgacg tggtcgcgca gcaacgcgtc gagatcctcg
      721 gcagtgcgcg tgtcacgggc agcttggccg gcaactcgat cgccgtcgcc gagggcgcga
      781 tcatcgaggg tgagatcaaa gtcgcgagcg gcgcgtcgcc gacgacgttc caggaaaagc
      841 gccaggccta aagcggcctc acgaggcctc gagctcggcg agcacggcac gatcgagcca
      901 gctgcgcaga ccatagtcct cgagaaacgc gcagtgcccg ccgcgcggca cgacgtcgat
      961 cttcagcgcc ggtgtgacgg cgacatccgc gagatcagcg atcggaatga cgggatcatc
     1021 ggcggccgcg atcagccgcg ccggcacttc aaggcgcgcg agcgcgtcgc ccgtgatcgc
     1081 gtagccgttc aagtacgact cgagatccgg aaagcgcgca taccgcgtcg caaagaattc
     1141 cgtcgtcgta gtcaacgtcg caaaccgccg gagatcgccg aagtcgtaga gctcggggaa
     1201 gcaggcggcc ttcgcgagca ggctgcggcg ccaacgccgc aagaaatact ggcgatacac
     1261 ccataagcct tcttcgagcg cgcgcatcgt gctcgtcggc cgcagcacgg ggcagatcgc
     1321 caccaccttg gtcacgggga tgtgtgcggc cggcgcacgc gccgcgacgc gtaaggcaaa
     1381 attaccaccc aacgagtggc cgacaatgaa cgtacgatcg gccggatgcc gcactgcgat
     1441 ctcgccaacc gcgccaacga cctcgtcgat gcggcacgaa tgaaacaaat cgcggttcag
     1501 ctcgaacgtc tcgccgtgat cgcggaaatt cagacgaaac acggagtaac cggcttcgaa
     1561 cagcagactg ccggcggaga tgacgtagct ggcggcagcg ctgccctccc agccgtgcag
     1621 cagaatcgca agacctttgg cggcgccggg ttgcgcgctg tagtggccga gtaagcggac
     1681 gccgtcgccg cagtcgacga tctcgtcgcg cgacgcggcc agcaccctcg ccgcgcgccg
     1741 ccgcaaccac cgttggcgcc aaggccagcc cgccagcaac gactggacgt ggcgatgcct
     1801 caggccgacg ggcgggtcga atgcttgagc ggcgtgcggc aaacgcttcc tcgtactaga
     1861 ctgacgggtg gttggcagac ctcctcggcc acgcggtagg cgaggagtac tgcagatata
     1921 acaataacaa tagtagagcc aatcgagccc accgccagac tatgctcgcg acgttcatca
     1981 attcgccgct gaagcgccgg ttcatggcgg ccgtcctgct gttggccggc tcggtgctgc
     2041 ttgcgctcgg cggcttggtc tacctttacg tcgcgggcaa cttgtcgctc gctctattcg
     2101 gcggcttggc gttcatctgc atcgccgcgg ccaccgtggt cggattgtat ctcgtgcgga
     2161 cgatcatcga tcgtttctcc gcgccgctgc gtacggtcac ggcctcgctg caaagcgcca
     2221 atggtgagct cgacgcggcg cgctttgccg acacgacgga gcttcgtgag ctcgcggcgg
     2281 ccgtcaatgc gatgcgcacg aatctgcgct cgagcacgat ctcgcgcgac tacctcgatc
     2341 gcttactgtc gagcatgggc gaagcgctgc tgatcaccaa cgccgaaggc aaggtcgagc
     2401 gcgccaacgg cgccgccgtc gagctgttcg ggcgaaccga agaggcgttg atcggcaagc
     2461 tcgccgacga cctgatcctg tccaacgacc gccggcgcgc cgagggcgcc gcgtcgcggc
     2521 cgcgcgaagg cacggtgctg cgacccgacg gcacgaccgt cagcgtgtcg tacacggtcg
     2581 cgaacgtgca cgacggcgac gcgctgcaga gcaaggtata cgcggcccac aacatcgacg
     2641 agcgcaaacg agtcgagcag cgcatccgct atctcgctcg taccgactcg ttgacgaaga
     2701 tcgccaatcg gatgcagttc cagcacttgc tgcagcaggc gatcgcgcgc gcgcgccgga
     2761 cgcagcagta cgtcgccatc gtttacttgg atgtcgaccg cttcaaagac atcaacgaca
     2821 cgtttggcca tgcggccggc gacacgagcc tcgagatctt cgcgcggcgc gtgctggcgg
     2881 agctcggcga gagctcgcac gcggggcgct tggcaggcga cgagttcgcc gtgctgatca
     2941 cgggcttcga aaggctcgac gcgatgcagc gtcacctgaa cgagcttgcg ccgcggttgc
     3001 tgacggcgac cggcaaaccg tttcaagtgc acggcgaaga aattttcctt acagcgagta
     3061 tggggatcgc cgtctacccg cgcgacggcg acaacgtcat cgatctgatc cgcaacgccg
     3121 acgcggcgct gtacgaagcg aaaaaagccg gcgggaactg cttcgagtac tactcgagcg
     3181 atatgaacac cgaggccgtc gagcgcctga tgctgaagag caagctgcgc cgcgcgttcg
     3241 agcgcgacga gctgcgtctg cactatcagc cgaaataccg aatttcgacg ggccgcatcg
     3301 aaggcgtcga agcgttgttg cgctgggact tgcccgagcg cggcctcgta tttccgtcgg
     3361 acttcgtacc gctcgccgag gagaccaatc tcatcctgca gctcggcgat tgggtgttga
     3421 accgcgtctg cgcggactat cgcgcgtggc agcgctccgt gccctcgcct tgccgcgttt
     3481 cgctgaacct gtcgctgcgg caactgcagc aacgccgctt tctcgatcaa gtgcgcgata
     3541 cgttccgcgc ccatggcgtg tcgccgacat cactcgagct cgagatcacc gagacgacgc
     3601 tgatggaaga ccccgagcgc acggtgcgca tcttggacgc gctgtacggc atggggctgc
     3661 acctcgcgat cgacgacttc ggcacgggtt actcgtcgct ctccgcgttg cagcaattcc
     3721 cgatcaatac gctgaagatc gatcagtcgt tcgtgcgcga cgtcgccatc gaccgcgacg
     3781 acgcggcgat cgtcgatgcg atcattcaaa tggcgcacag cttgcggctc gaggtcgtcg
     3841 ccgaaggcgt cgagtcggaa gcgcagctcg agtttctgcg tagccacggc tgcgaccacg
     3901 cacaaggtca cttgttcggc gaccccgtca cggcggacca gttctgcgag ctgttgctcg
     3961 cggaatcggc gggcaccggc aagtaccgcg cactgttcgc gggcgccggc gtgtaatgcg
     4021 ctccgcgcga cgactaggta tagtttgttc aatcactgtc ttgagtgcga cgaggtaatt
     4081 atgaaggtgc tgacttccgg cctggtggcc gccattgcga cgggctacgg tgtcgccgcc
     4141 ggggcttgcg agaatccgac tgccgttgcg atccccgacg gcaagagcgc gacgatggag
     4201 cagatgttgg ctgcgcaagc gcaggtcaag gcctaccaaa ctgcgatgaa cgcgttcttg
     4261 gcgtgcctcg ataccgagct cgatgctcag ggcgagcaag cgccggaaga cttcaagtca
     4321 ctgatggtct cgcgacacaa tgcggcggtc tccgagatgg aaggtgtcgc cgccgcgttc
     4381 aacgatcaga tcaaggccta tcgcgcagcg aatccggcgc cccccaaatg atcgcgtagc
     4441 ttcgtttggc tgagcgcggc gccctcgagg cgccgcgttc gttttcaggc cccgagatcc
     4501 ttgaactgtt gcttcagctc gtagcgctcg tccgtcacga ttcgctcgag cacgcgcacg
     4561 cgctcttcca cttgatgcag ccgctcgagc agatcggtgt tgacgccatc gtcgcgcggc
     4621 ggctgcgcgt tcttgtgagc cattgccgtc ctgatcaact tgaaaatcaa aacgagcgcg
     4681 accatggcga gcgcgaactc aaacggattc atccgtggtg acctccccaa aacaccgcaa
     4741 acgacgccga aacttttact tcccgagctg ctcgaactcg cgatcgagct tgtaacgatt
     4801 cgaggtcacg tatttctcga ggcgctgcaa gcgcacgtcg agatcacgaa accgatagcg
     4861 cacgctggca agcgtgtcat gcgggctcga acgcacttgg cgtcgaatag accgctcctc
     4921 gcgctcggcc gggtcgttgt agtcgcggtc cccgtccgaa tacctgcgcg gcggcaagag
     4981 caagccgagc agtagataca cgacgcagat gacgggaaac gcgaagaacg ccgtcgccgc
     5041 gacgatgact cgcgtgaccg taacgtcgaa cccgaagtac tcggcgatgc cggcgcagac
     5101 gccgaagatc atgccgcgcc gcggcaagcg gtacagcttg ttgatcaaac ccggctcgcg
     5161 tggtgattcg ttcactgctt gtttctccag tccggaactt cgctgtcgag aatcgtctcg
     5221 agcgcgttca ctcgactctg catcccttgg ctttctttcc aaagatcctc gagca
//
LOCUS       DATLEE010000314         2375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4068899, whole genome shotgun sequence.
ACCESSION   DATLEE010000314 DATLEE010000000
VERSION     DATLEE010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4068899"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /gene="rlmKL"
                     /locus_tag="VM692_06730"
     CDS             complement(<1..1449)
                     /gene="rlmKL"
                     /locus_tag="VM692_06730"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HUQ51899.1"
                     /translation="MHSFYVTVPAGTADLAAEELAACGIVEARVERGGVGCSGTLEQA
                     YRACLWSRVANRVLLKVAEFPAPTPEALYDGVRTVDWSEHLAVDRTLAVDCTSTRSAI
                     THTQYAALKVKDAVVDQFRERTGSRPSVDTTAPDVRLNLHLDRDVATVAIDLSGDSLH
                     RRGYRGAQGAAPLKENLAAAVLLRAGWAKLVAAADGELGFVDPMCGSGSLAIEAALIA
                     ADVAPGLLRNEFGFLTWRGHDEAAWQRLLTEAAERRSAARAERVVFLAYDRDSAAVRA
                     TLENAARAGFGKLVHAERRELADLPAAPAPRGLLAVNPPYGERLGEADALQGVYELLG
                     DKLRELYVGWQAAVLTGNPPLGRELKLKAKRTHTMYNGPIECRLLRFDVEPKHFEQKR
                     APGSLPIFDAEAARARPGAAMFANRLRKNAESLGDWARKEGIACYRAYDADMPEYAFS
                     IDVYGASVEPTAQRFVYVQEYAPPATIDPDKAR"
     gene            complement(1531..>2375)
                     /locus_tag="VM692_06735"
     CDS             complement(1531..>2375)
                     /locus_tag="VM692_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51900.1"
                     /translation="LGPVVTGKTSLLRAQRRWADARGVRYDAYGCVRDLADNLRAPLA
                     GAALAELERGSELTPYATRPARLYSLGSSAALVLNVFDFWRERDTSPLLQALDVAGDG
                     AVRLKLEEPLPTGLPGDAPTADVLLELGGGRYVAIESKYAEWLVRRPRNKRVFKDKYF
                     PAGAGQGVWSAAGLPRCQELAEDLQAGRQRPKYLHAPQLLKHALGLAHSGRRNSVLVY
                     LYFDWPVREAETHRAEIERVVARLTPEIDLRVLTYQTLFASLRAAPGVDADYLDYLAQ
                     RYFR"
BASE COUNT          367 a          848 c          815 g          345 t
ORIGIN      
        1 gcgcgcctta tcaggatcga tcgtcgccgg cggcgcgtac tcctgcacgt aaacgaaacg
       61 ctgcgcggta ggctcgacgg acgcgccgta gacgtcgatc gagaaggcgt actccggcat
      121 gtcggcgtcg tacgcgcgat agcaggcaat gccttccttg cgcgcccagt cgccgagcga
      181 ctcggcgttc ttgcgcagac gattcgcgaa catggcggcg cccggtcgcg cgcgcgcggc
      241 ctcggcatcg aatatgggca acgagcccgg cgcgcgcttc tgctcgaagt gcttcggctc
      301 gacgtcgaac cgcagcaagc ggcattcgat cggtccgttg tacatcgtgt gcgtgcgctt
      361 ggctttgagc ttcagctcgc ggccgagcgg cggattgccc gtcagcacgg ccgcctgcca
      421 gccgacgtag agctcgcgca gcttgtcgcc gagcaactca tagacgcctt gcagcgcgtc
      481 ggcctcgccg agccgctcgc cgtacggcgg gttgacggcc aacaggccgc gtggcgccgg
      541 cgccgcgggc aggtcggcga gctcgcgccg ctccgcgtgc acgagcttcc cgaacccggc
      601 acgcgccgcg ttctcgagcg tggcgcgcac cgctgccgag tcgcggtcgt aagcgaggaa
      661 caccacgcgc tcggctcgcg cagccgaacg ccgctcggcc gcttccgtca gtaggcgctg
      721 ccacgcagcc tcgtcgtggc cgcgccacgt caagaagccg aactcgttgc gcagcaagcc
      781 cggcgccacg tccgccgcga tcaacgccgc ctcgatcgcg aggctgcccg agccgcacat
      841 cgggtcgacg aagccgagct cgccgtcggc agcggcgacg agcttcgccc agccggcgcg
      901 cagcaacacg gccgcggcga gattctcttt cagcggcgcc gccccctgcg cgccgcgata
      961 accgcgccga tgcaagctgt cgcccgagag gtcgatggcc acggtcgcga cgtctcgatc
     1021 gaggtgcaga ttcagccgca cgtcgggcgc ggtcgtgtcg acggacggcc ggctacccgt
     1081 ccgttcgcga aactgatcga cgacggcgtc tttaactttc agcgcggcgt actgcgtgtg
     1141 ggtgatggcc gagcgcgtgc tcgtgcaatc gacggcgagc gtcctgtcga ccgcgagatg
     1201 ctcgctccag tcgaccgttc gcacgccgtc atagagcgcc tccggcgtcg gggccgggaa
     1261 ctccgcgact ttcaacaaca cgcgattcgc gacgcgcgac cagaggcacg cgcgataggc
     1321 ctgctcgagc gtgccgctgc agccgacgcc gccgcgctcg actctcgcct cgacgatgcc
     1381 gcaagccgcc agctcttcgg ccgccaggtc cgcggttccg gccggcacgg tgacgtagaa
     1441 agaatgcaaa gcgcgaccta gtgtgatttc ggctgacggc gggtggccgc catgctaagc
     1501 gatgcacccg gcgaacgcac gccgcgcgcc tcagcggaaa tagcgctgcg cgagatagtc
     1561 gagatagtcg gcgtcgacgc cgggcgcggc gcgcagcgac gcgaacaacg tttgatacgt
     1621 cagcacgcgc aagtcgattt ccggcgtcag ccgcgcgacg actcgctcga tttcggcgcg
     1681 gtgcgtttcg gcttcgcgca cgggccagtc gaagtacaga tagacgagca cgctgttgcg
     1741 ccggccgcta tgagcgaggc ccaacgcatg cttcagcagc tgcggcgcgt gcagatactt
     1801 cggccgctgg cggccggcct gcaggtcctc cgcaagctcc tggcaccgcg gcaacccggc
     1861 cgccgaccag acgccttggc ccgcgcccgc tgggaagtac ttgtccttga agacgcgctt
     1921 gttgcgcggg cggcgcacga gccactcggc gtacttgctc tcgatcgcga cgtaacggcc
     1981 gccgccgagc tcgagcaaga cgtcggccgt cggcgcgtcg ccgggcaagc ccgtcggcaa
     2041 cggctcttcg agcttgagcc gcaccgcgcc gtcgccggcc acgtcgagcg cttgcagcaa
     2101 cggcgacgtg tcgcgctcgc gccagaagtc gaacacgttg agcacgagcg cggcggacga
     2161 gcccagcgaa tagagccgag ccggccgcgt cgcgtacggt gtcagctcgg agccgcgctc
     2221 gagctctgcg agcgccgctc cggcgagcgg cgcgcgcagg ttgtcggcga gatcgcgcac
     2281 gcagccgtac gcgtcgtagc gcacaccgcg agcgtcggcc cagcggcgct gggctcgcaa
     2341 caaactggtt ttgccggtga ccaccggccc gagtc
//
LOCUS       DATLEE010000315         2048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1849766, whole genome shotgun sequence.
ACCESSION   DATLEE010000315 DATLEE010000000
VERSION     DATLEE010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849766"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..>2048
                     /locus_tag="VM692_06740"
     CDS             271..>2048
                     /locus_tag="VM692_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ51901.1"
                     /translation="MDTLFDDLRYAARLLLKHRRFTLIAVLSLALGIGANTAIFSTVS
                     AVLLRALPFHEPDRLVIVWEDASFAGFPRNTPAPANYADWKAQSTAFEDMAAIRERTF
                     NLTGDGEPEKVQAHAITANLFALLGVQPAVGRYFAPEEDSPGATVVVLSHRLWQTRFG
                     GRTDIVGREILLDGQQHRVVGVMPREFQFLDDTVGLWTPLGLTADDLANRSSHYLTVV
                     ARTKPGVSLGQAQADIDTIMTRIARDYPDAARGLGGLVLPLHEQLTGDMRRPLIALQI
                     AVGLVLLICCSNIANLFLSRTTARRREIAMRTSLGASRWRVVRQLLVESVLIAAVGGV
                     LGIVLAWWSFGFLQQMIPPGMRLYTPVQLDAGVLAFAVVACLLTGIGFGLAPALQAAN
                     VDLNEAMKADGGHSHLVKQNRLRNTMVVAQVALALVVLVGSGLLVRTINALEEQYSSM
                     RPERLLTLRTDLPLNKYDEPQKRTNFYDEVLTRVQSLPDVSSAAYTTSVPLEWKGGTT
                     GFYPEGKEVMPGLTYDANHRQVSSGYLATLGIALVGGRHFEESDRLQTQPVAIVNETM
                     ARQFWPGEDALGKRFKLGAPDSEEPWR"
BASE COUNT          344 a          679 c          667 g          358 t
ORIGIN      
        1 acgctgcgga tccaggtcga ggcgttggca gccagctaag cgctctcctc gatctctcga
       61 aacgacaagg ccgggcgctg cgaggcgctc ggcctttttt tcgtccgcgc ggcgctcccg
      121 tcccgcgatc gagacggcgg cgcgattgtg ggatgcgccg ggctcactgc tcgcaacatg
      181 agcgctagag caatcaacgc cttgcgtggc tgattcgatt ggcacgcacg ttgcttgacc
      241 accgcgtgca aacaaggacg tgacggaata atggacacac tttttgacga tctgcgctat
      301 gccgctcgat tgctgctgaa gcatcggcgg ttcacgctca tcgccgtgct gtcgctcgcg
      361 ctcggcatcg gcgctaacac ggcgatcttc agcaccgtca gcgcagtgtt gctgcgagcg
      421 ctccccttcc acgagcccga tcgcttggtc atcgtttggg aagacgcatc gttcgccggc
      481 ttcccgcgta acacaccggc tccggcgaac tatgcggact ggaaagctca gagcacggcc
      541 ttcgaggaca tggccgccat acgcgagcgc acgttcaacc tgacgggcga tggcgagccg
      601 gagaaagtgc aagcgcacgc catcacggcg aatctatttg cgttgctcgg cgtgcagccc
      661 gccgtcggac gttacttcgc tcccgaggag gacagccccg gcgcgaccgt cgtcgtgctg
      721 agtcatcggc tctggcagac gcggttcggc ggccgaaccg acatcgtcgg ccgagagata
      781 ctgctcgacg gccagcagca ccgcgtagtc ggcgtcatgc cgcgcgagtt tcagtttctc
      841 gacgacaccg tcggcctctg gacgccgctc ggcctcacgg ccgacgacct cgcgaaccgc
      901 tcgtcgcatt acctgaccgt cgtcgcgcga acgaagcccg gtgtgtcgct gggtcaggcg
      961 caggccgaca tcgatacgat catgacgcgc atcgcacgcg actatcccga cgcggcgcgc
     1021 gggctcggcg gcctcgtgct gccgctgcat gagcaactga ccggcgacat gcgccggccg
     1081 ctgatcgcgc tgcagatcgc cgtgggtctc gtgctgctga tctgctgcag caacatcgcc
     1141 aacttgttcc tgtcgcgcac gacggcgcgg cggcgcgaga tcgcgatgcg gacgtcgctc
     1201 ggagcgagcc gctggcgcgt cgtgcggcaa ctgctcgtcg aaagcgtgct gatcgccgcc
     1261 gtcggcggcg tgctcggcat cgtgctggcc tggtggagtt tcggtttctt gcagcagatg
     1321 atcccgcccg gcatgcgcct ctacacgccc gtgcagctcg acgccggcgt gttggccttt
     1381 gcggtcgtcg cctgcctgct gaccggtatc gggttcggcc tcgcgccggc gctgcaagcg
     1441 gctaacgtcg acctcaacga agcgatgaag gccgacggtg gccacagcca cctcgtgaag
     1501 cagaatcgct tgcgcaacac gatggtcgtc gcgcaggtcg cgctggcgct cgtcgtgctc
     1561 gtcggctccg gcttgctcgt cagaacgatc aacgcgctcg aagagcagta ctcgagcatg
     1621 cgtcctgagc gcttgttgac cctgcgaacg gacttgcccc tgaacaagta cgacgagccg
     1681 caaaagcgca cgaacttcta cgacgaggtg ctgacgcggg tgcaatcgct gcccgacgtc
     1741 agctcggcgg cctacacgac gagcgtgccg ctcgaatgga aaggcggcac gaccgggttc
     1801 taccccgagg gcaaagaggt catgccgggg ttgacgtacg acgccaacca ccggcaagtg
     1861 agctccggtt atctcgcgac gctgggcatc gcgctggtcg gcggtcgcca cttcgaagaa
     1921 agcgaccggt tgcaaacgca gcccgtcgcg atcgtcaacg aaacgatggc gcggcagttc
     1981 tggcccggcg aggacgcgct cggtaagcgc ttcaagcttg gcgctccgga ctccgaagag
     2041 ccgtggcg
//
LOCUS       DATLEE010000316         4485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5582637, whole genome shotgun sequence.
ACCESSION   DATLEE010000316 DATLEE010000000
VERSION     DATLEE010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5582637"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VM692_06745"
     CDS             <1..988
                     /locus_tag="VM692_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HUQ51902.1"
                     /translation="VAEDLRKLLPEASVRVGHGQMSERELELVMLDFYHRQFNVLVCT
                     TIIESGIDIPTANTIVINRADRLGLAQLHQLRGRVGRSHHQAYAYMLAPPRNALTPDA
                     LKRLEAIESLEELGSGFTLATHDLEIRGAGELLGEGQSGQIQAIGFTLYNELLGRAVA
                     ALRAGEEPDLEDPFTHGPEIEIGLAALIPEDYMPDVHMRLVHYKRIANCRNNLELDNL
                     KVELIDRFGLLPPPLKTLFSITALKLLAAQLGIAKIQAGSEAGSITFAERSRVDPTRL
                     LKLIQSDTARYRLDGPYKLRFSWTLENPEKRIAALEKLLRRLGAEDTAAAAA"
     gene            1006..1767
                     /locus_tag="VM692_06750"
     CDS             1006..1767
                     /locus_tag="VM692_06750"
                     /inference="COORDINATES: protein motif:HMM:NF022425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HUQ51903.1"
                     /translation="MKSRALSLVLSLLVAAVAGAQAPVPSPAEAALPPRYEVEIIVFA
                     NRELDPTEERFKQELDGFEGGAETLRDAPVFDDTNFGAAARTEPLQAPSDLLTPADPL
                     AAQLAEALRVRPLKPEELKLNNEYRKLRAVSAYVPLVHAGWVQPGLPEADAQPFDLKV
                     LGILNPSGTIRVHLERFLHITLDLTYRADGTAGTAPAVGDGLDELVAEPRYHLKATRS
                     ARSGELHYFDHPAFGVLVRVTPVPTQNAQGRRPAA"
     gene            complement(1788..2792)
                     /locus_tag="VM692_06755"
     CDS             complement(1788..2792)
                     /locus_tag="VM692_06755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUQ51904.1"
                     /translation="METGRDARGIRSLNRHTLEQIVGLSPAAILVADALDPRLPIVYA
                     NGAYERLSGYSADELCGHPWAVLARAADGDGSLAALRAAIGRGEACRATLPDLKKDGT
                     SWTSEVSVAPLSGPRGDLRFFLLTHEPVTAVAASRDTAAPPFAPVDSSGEISLLQREL
                     GRARQKIATMDRLDPATGLVRFAHFQETLRRDLAMARRDRRFVTLLVFEIVEYAAYRQ
                     TFGDKAADSCQRMIGAQIMSALRRAGDLCARYDESTLVAAAVGQHADEIRPLVDRIAD
                     GVRQLKLHNPRGKTSRYITTRVSLISCPPGAHDDPEPLIARALTEARSNEVPVRAILG
                     "
     gene            complement(2838..3584)
                     /locus_tag="VM692_06760"
     CDS             complement(2838..3584)
                     /locus_tag="VM692_06760"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HUQ51905.1"
                     /translation="MTRLALIVGSGFEHLGFEVLTRSPTKTPYGEPSSSVLTIAVGGA
                     RVACIARHGEGHALAPHEINYRANVWALQQRGVRWCVGINTVGSIDLGLVPGGLAVPA
                     QLIDYTHGRAATFGGGGSAVRHIEFAEPFSPALRGRLATAILDCGLVVRAGTYGVTQG
                     PRLETAAEIERLARDGCTMVGMTAMPEAALARELDLEYAVCAVAVNYAAGRSPDGAPI
                     TGQIERFMTAGLAKVRPVLERLVAGIEDAV"
     gene            complement(3581..4141)
                     /locus_tag="VM692_06765"
     CDS             complement(3581..4141)
                     /locus_tag="VM692_06765"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF006605.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HUQ51906.1"
                     /translation="MAAVPASAQTALRASRRIYSARDVARALDRMAAKLRGRLEHSNP
                     VVLAVMHGGAFAALELCKRFDFPHEFDYVHVTRYRGETSGHELRWRVRPSKALAGRTV
                     LVVDDILDHGTTLRALESELDRVGVEQRFSAVLVVKRLKRRRSRPRVTVSGLAVEDVY
                     VFGSGMDYRGYWRAQPGIYALADADA"
     gene            complement(4143..>4485)
                     /locus_tag="VM692_06770"
     CDS             complement(4143..>4485)
                     /locus_tag="VM692_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HUQ51907.1"
                     /translation="NQYLRGELEFKGAVISDALSMVGASVVGPPATRVRLALEAGCDL
                     VLLCNSPNEIPPVLAALEGYVNPTAQLRLTRLHGRGELTWDGVHGSAEWHKASALVAP
                     LCARPKLELEG"
BASE COUNT          747 a         1583 c         1471 g          684 t
ORIGIN      
        1 agtcgccgaa gacctacgca agctgctgcc ggaggcttcc gtacgcgtcg ggcacggcca
       61 gatgtcggag cgcgagctcg agctcgtcat gctcgatttc taccatcgcc agttcaacgt
      121 gctcgtctgc acgacgatca tcgagagcgg catcgacatt ccgaccgcga acaccatcgt
      181 catcaatcgc gcggatcggc tcggcttggc gcagctgcat cagctgcgcg gccgcgtcgg
      241 ccgctcgcat catcaggcgt acgcgtatat gctcgcgccg ccgcgcaacg cgctgacgcc
      301 cgacgcgctg aagcgcctcg aggccatcga gtcgctcgag gagctcgggt ccggcttcac
      361 gctcgcgacg cacgacctcg agattcgcgg cgccggcgag ttgctcggcg aaggtcaaag
      421 cggccaaatt caggcgatcg gcttcacgct ctacaacgag ctgctgggac gcgccgtcgc
      481 ggctttgcgc gccggcgagg agcccgacct cgaggatccg ttcacgcacg ggcccgagat
      541 cgagatcggg ctcgcagcgc tgattcccga ggactacatg cccgacgtgc acatgcggct
      601 cgtgcattac aagcgcatcg caaattgccg caacaatctc gagctcgaca acctcaaggt
      661 cgagctcatc gaccgcttcg gcctactgcc gccgccgttg aagacgctgt tctcgatcac
      721 ggcgctgaag ctgctcgccg cacaactcgg cattgcgaag atccaggccg gcagcgaagc
      781 cggcagcatc acgttcgccg agcgctcgcg cgtcgacccg acgcggctgc tcaagctgat
      841 tcagagcgat acggcacggt atcggctcga cgggccctat aagctccgct tctcgtggac
      901 gctcgaaaac cccgagaaac gcatcgccgc cctcgagaaa ctgttgcggc gcttgggcgc
      961 cgaggacacg gccgccgccg cagcctgaga cgctatactg ccgccatgaa atcgcgcgcg
     1021 ttgtcgctcg tcttgtcgct gctcgtcgct gccgtcgccg gggctcaagc gcccgtgccg
     1081 tcgccggccg aggctgcgct gcctccgcgg tacgaggtcg agatcattgt cttcgcgaac
     1141 cgcgagctcg atccgaccga ggagcgcttc aagcaagagc tcgacggctt cgaaggcggc
     1201 gccgagacgc tacgcgatgc gcccgtcttc gacgatacga acttcggcgc cgcagcgcgg
     1261 accgagccgc tgcaggcgcc gagcgacctg ctgacacctg cggatccgct ggcggcgcag
     1321 ctcgccgaag cgctgcgggt ccggccgctg aagcccgagg agctgaagct caacaacgaa
     1381 taccgcaagc tgcgcgccgt ctcggcgtat gtgccgctcg tccacgccgg ctgggtgcag
     1441 cccgggctgc cggaagccga cgcgcaaccg ttcgatctca aggtgctcgg catcttgaat
     1501 cccagcggaa cgattcgcgt gcatctcgaa cgcttcctgc acatcacgct cgacttgacg
     1561 tatcgagccg atgggaccgc gggcaccgcc ccggccgtcg gcgacgggct cgacgagctc
     1621 gtggccgagc cgcgctacca cttgaaggcg acgcgcagcg cgcgcagcgg cgagctgcac
     1681 tacttcgatc atccggcgtt cggagtgctc gtgcgggtta cgcccgtccc cacgcagaat
     1741 gcccaaggcc gccgtcccgc ggcctgactg ccgccgtccc gcccctgtca accgagaatc
     1801 gcgcgaaccg gaacctcgtt actacgagct tccgtcagtg cgcgcgcgat caacggctcg
     1861 ggatcgtcat gcgcgccggg aggacagctg atgaggctga cgcgcgtcgt gatgtagcgg
     1921 ctcgtcttgc cgcgcggatt gtgcagcttc agctgccgga cgccgtcggc gatgcgatcc
     1981 acgagcggcc gaatctcgtc ggcgtgctgg ccgacagccg cggccaccaa cgtcgactcg
     2041 tcgtaacgtg cgcagagatc gccggcccgg cgcaacgcgc tcatgatttg cgcaccgatc
     2101 atgcgctggc aggagtccgc ggctttgtcg ccgaacgttt gtcgatacgc cgcgtactcg
     2161 acgatctcga acacgagcag cgtcacaaac cgacggtcgc ggcgcgccat ggcgaggtcg
     2221 cgccgcagcg tctcttggaa atgcgcaaag cgcacgagcc ccgtcgccgg atcgaggcga
     2281 tccatcgtcg cgatcttctg tcgcgcgcgg ccgagctcgc gctgcagcaa ggagatctcg
     2341 ccgctgctat cgaccggcgc gaacgggggc gcggccgtgt cgcggctcgc ggcaacggcc
     2401 gtaaccggct cgtgagtcag caagaagaag cgcaagtcgc cgcgcggacc gctcagcggc
     2461 gcaacgctga cttcgctggt ccacgacgtg ccgtcttttt tgaggtcggg tagcgtcgca
     2521 cggcaggcct cgccgcggcc gatcgccgcg cgcagcgcgg ccagcgagcc gtcgccgtcc
     2581 gccgcgcgcg caagcacggc ccacgggtga ccgcagagct cgtcggccga gtagccgctc
     2641 aagcgctcgt aggcgccatt cgcatagacg attggcaggc gtggatccag agcgtcggca
     2701 acgagaatgg ctgccgggct caagcccaca atctgctcta gagtgtggcg gttcagggat
     2761 ctgatgccgc gcgcgtccct gccggtttcc attcgattat gttcgatgac gcgcgcccgc
     2821 aacgtctatg aaacgcatca cactgcatcc tcgatgccgg cgacgagacg ctccagcacg
     2881 ggccgcacct tcgcgagacc tgccgtcatg aaccgttcga tttgtcctgt gatcggcgcg
     2941 ccgtccggcg accggccggc cgcatagttg accgcgacgg cgcaaacggc gtactcgagg
     3001 tcgagctcgc gagccaacgc ggcttcgggc atcgcggtca tgccgaccat cgtgcaaccg
     3061 tcgcgggcca accgctcgat ttcggcagcc gtctcgagac gcgggccctg cgtcacgccg
     3121 taggtgccgg cacgcacgac gagcccgcaa tccaggatcg ccgtcgcgag ccgcccgcgc
     3181 aacgccggcg agaacggctc cgcgaactcg atgtgccgca cggccgaacc gccgccgccg
     3241 aaggtcgcgg cacggccatg cgtgtaatcg atgagctgag cgggaaccgc gagcccgccc
     3301 ggcactagac ctaaatcgat cgaaccgacc gtgttgatgc cgacacacca gcgcacgccg
     3361 cgttgctgaa gagcccaaac gttcgcgcgg tagttgatct cgtgcggagc gagcgcgtgg
     3421 ccctcgccat ggcgagcgat gcacgcgacg cgcgcgccgc cgacggcgat cgtcagcacg
     3481 ctgctgctcg gctcgccgta cggagtcttc gtcgggctgc gcgtcaacac ttcgaacccg
     3541 aggtgctcga agccgctacc gacgatcagc gcgaggcgcg tcatgcgtcg gcgtccgcaa
     3601 gcgcgtagat gccgggctgc gcgcgccagt agccgcgata gtccatgccg ctgccgaaca
     3661 cgtagacgtc ctcgacggcg agcccgctga ctgtgacgcg cggcctcgac cgtctgcgct
     3721 tcagacgctt gacgacgagc accgcgctga agcgctgttc gacgccgact cgatcgagct
     3781 cgctctcgag ggcgcgcagt gtcgtgccgt gatcgaggat gtcgtcgacg acgagcacgg
     3841 tcctgcccgc cagcgctttg ctggggcgca cgcgccatct gagctcgtgc ccgctcgtct
     3901 cgccgcgata gcgcgtcaca tgcacgtagt cgaactcgtg cggaaagtcg aaccgcttgc
     3961 agagctcgag cgctgcgaag gcgccgccgt gcatgacggc caatacgacg ggattcgaat
     4021 gctcgagccg ccccctgagc ttcgcggcca tgcgatcgag cgcgcgcgcg acatcgcgcg
     4081 ccgaatagat gcgtcgcgac gcgcgaagcg cggtctgcgc cgacgcggga accgccgcca
     4141 cgtcagccct cgagctcgag cttcgggcgc gcgcagagcg gcgccaccag cgcgctcgct
     4201 ttgtgccact cggccgaacc gtgcacgccg tcccacgtca gctcgccgcg gccgtgtagg
     4261 cgcgtgaggc gcagctgcgc cgtcggattg acgtagccct cgagcgccgc gagcaccggc
     4321 ggaatctcgt tcggcgagtt gcacagcagc acgagatcgc agcccgcctc gagcgcgagg
     4381 cgcactcggg tcgccggcgg ccctacgacc gacgcgccga ccatgctcaa ggcgtccgag
     4441 atgacggcgc ctttgaactc gagctcgccg cgcaagtact ggttg
//
LOCUS       DATLEE010000317         5287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2601466, whole genome shotgun sequence.
ACCESSION   DATLEE010000317 DATLEE010000000
VERSION     DATLEE010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601466"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /gene="recA"
                     /locus_tag="VM692_06775"
     CDS             <1..923
                     /gene="recA"
                     /locus_tag="VM692_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HUQ51908.1"
                     /translation="NLDAATGIGGVPRGRITEIYGPESSGKTTLCLHLVASVQKMGGV
                     AAYVDAEHALDVTYARKLGVRTDDLLISQPDTGEQALEIADALVRSGGIDLLVVDSVA
                     ALVPRAEIEGEMGDSHMGLQARLMSQALRKLTGSINRSKTLLIFINQIRMKIGVMFGN
                     PETTTGGNALKFYASVRLDIRRTGSIKKGEEVIGSETRVKVVKNKVAPPFKTAEFEIL
                     YGLGISREGEIVDLGVKQNIIEKSGAWYSFNGDRIGQGKENARDFLIEHKDIAAAIEK
                     RVRAELLPAAKKGPAEAPAAAEEEGEALEA"
     gene            904..1386
                     /locus_tag="VM692_06780"
     CDS             904..1386
                     /locus_tag="VM692_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011535188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HUQ51909.1"
                     /translation="MRRSKPDAGELDAPACRRIAIELLARREHSRRELTRKLSSRGFP
                     EDVIAGVLDELEQSGELADARFTDTFVRSRVAKGRGPQRIRAELAQRGIGDSAADEVL
                     RGAEVDWLETVRAVRCKRFGAELPRDYAERARQARFLQYRGFSSEQIRAALELDADSD
                     "
     gene            1437..4061
                     /gene="alaS"
                     /locus_tag="VM692_06785"
     CDS             1437..4061
                     /gene="alaS"
                     /locus_tag="VM692_06785"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HUQ51910.1"
                     /translation="MSLTSSQLRQAFLDFFAKHGHRVVPSSSLVPGNDPTLLFTNAGM
                     VQFKDCFLGKDKRDYVRAASSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKKDAIRFAWDFVTGTLKIDPKRLMVTVYATDDEAYDLWHKMIGLPADKIVRIGDNKA
                     DGGSDNFWQMGDTGPCGPCTEIFYDHGPQVAGGPPGSADADGDRWVEIWNLVFMQFDR
                     SADGKLTPLPKPSVDTGAGLERIAAVMQGVTNNYDIDLFVELIREAARLLGTNDYKNP
                     SLRVIADHIRACSFLVVDGVIPSNEGRGYVLRRIARRAMRHGYKFGVSPAFFHELVPT
                     LAKVMGGAYPELVAKQKFVAEVLLKEGEQFARTLETGMEILDAAIGKLGNNKIIDGNT
                     VFKLHDTYGFPTDLTADIARERGLTIDTEGYERAMDVQRKQSQAASQFGVDLSAASAK
                     IDLKTEFLGYELLVSDAPIVALLDSEGNAVQALEAGAAGTVVLERTPFYAESGGQVGD
                     SGVLSSGAARFDVADTKKVGTAFGHHGKVTVGRVAIGDRVQAQVDAKRRASIVLNHSA
                     THLLHAALRKVLGAHVQQKGSLVEPDRLRFDFSHFEPVSAEQLKEIEALVNEQVRLNH
                     GADIQELPYEQAIAAGALAFFGDKYGDKVRVLKLGEFSTELCGGTHVARSGDIGLFKI
                     VSEGGIAAGVRRIEAVTGQGALDVVSANEAVLREVASLVKANRDDVAAKVTQLVERAR
                     SLERELQTLRQKLASGGGRDILKEAKVVNGINVLVARLDGADAKALRDTADQLKSKLG
                     SAVVVLGAVEGDKVFLVASVTQDLAGRLKAGEIIKPIAELCGGRGGGRADFAQAGGTK
                     PDKVDAALALVPELIAAA"
     gene            4362..4574
                     /gene="csrA"
                     /locus_tag="VM692_06790"
     CDS             4362..4574
                     /gene="csrA"
                     /locus_tag="VM692_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HUQ51911.1"
                     /translation="MLILTRRVGETVVIGNDVTVTVLGVKGNQVRLGVNAPREVAVHR
                     EEIFERIKREQSEEQPRPHLNGTAHP"
     gene            4678..4768
                     /locus_tag="VM692_06795"
     tRNA            4678..4768
                     /locus_tag="VM692_06795"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4712..4714,aa:Ser,seq:gct)
     gene            complement(5009..>5287)
                     /locus_tag="VM692_06800"
     CDS             complement(5009..>5287)
                     /locus_tag="VM692_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51912.1"
                     /translation="ALFAQHEKTLGPLVLDPFGAIVVSECHGYEYCRTSREVPIPLGC
                     SPARRRPERPDDEGWQQPSNDQLIREQAAAEAVQSARRELLAAIARGD"
BASE COUNT         1000 a         1663 c         1738 g          886 t
ORIGIN      
        1 tcaacctgga tgccgccacc gggatcggcg gggtgccccg ggggcggatc accgagatct
       61 acggcccgga gtcgtcgggc aagacgacgc tctgcctcca cctcgtggcc agcgtgcaga
      121 agatgggcgg cgtggcggcc tacgtggatg ccgagcacgc gctcgacgtc acttacgccc
      181 gcaagctcgg cgttcgcacg gatgatctcc tgatctcgca gccggatacc ggcgagcagg
      241 ctctcgagat cgccgacgcg ctcgttcgct cgggcgggat cgaccttctg gtcgtcgact
      301 cggtcgcggc cctcgtgcct cgcgccgaaa tcgaaggcga gatgggcgat tcgcacatgg
      361 gtcttcaagc tcgcttgatg agccaggctc tgcgtaagct gaccggctcc atcaatcgca
      421 gcaagaccct cctgatcttc atcaatcaga tccgcatgaa gatcggcgtg atgttcggca
      481 acccggagac gacgacgggc ggcaatgcgc tgaagttcta cgcctcggtg cggctcgata
      541 tccgccgcac gggctcgatc aagaagggcg aagaggtcat cggcagcgaa acgcgcgtca
      601 aggtcgtcaa gaacaaggtc gccccgccgt tcaagaccgc ggagttcgag atcctctatg
      661 ggctaggcat ctcgcgcgag ggcgagatcg tcgacctcgg cgtcaagcag aacatcatcg
      721 agaagtccgg tgcgtggtac agcttcaacg gcgaccgtat cggccagggc aaggaaaacg
      781 cccgcgactt cctgatcgag cacaaggaca tcgcggccgc gatcgagaag cgcgtccgcg
      841 ccgagctcct gccggcggcc aagaagggac cggccgaggc gccggcggcc gccgaggagg
      901 agggtgaggc gctcgaagcc tgacgcgggc gagctcgatg cgccggcgtg caggcgcatc
      961 gccatcgagc tcctcgcgcg ccgcgagcat agtcgccgcg agctgacgcg caagctcagc
     1021 tcccgcggct ttcccgagga cgtcatcgcc ggcgtcctcg acgagctcga gcagtcgggc
     1081 gagctcgccg acgcccgttt cacggacacg ttcgtgcgct cgcgcgtggc gaagggtcgg
     1141 ggcccgcagc gcatccgcgc cgagctcgcg cagcgcggca tcggcgactc tgccgccgac
     1201 gaggtgttgc gcggcgccga agtcgactgg ctcgaaacgg ttcgggccgt ccgctgcaag
     1261 cgtttcggcg ccgagctgcc gcgcgactat gccgagcgcg cccgacaggc gcgcttcctg
     1321 caatatcgcg gcttctcgag cgagcaaatc cgcgctgcac tggaactcga cgccgattcc
     1381 gattagcgtg gcgttccgct ggtttttggt cgatctgtca attccgagcg taatcaatga
     1441 gtcttacgag tagtcagctg cgccaggcct tcctggactt cttcgccaag cacggccacc
     1501 gcgtggtgcc gagctcgtcg ctcgtgcccg gcaacgaccc gacgctcttg ttcacgaacg
     1561 ccggcatggt gcagttcaag gactgcttct tgggcaagga caagcgcgac tacgtccggg
     1621 ccgcgagcag ccaacgctgc gtccgcgcgg gtggcaaaca caacgacctc gagaacgtcg
     1681 gctacacggc gcgccatcac acgttcttcg agatgctcgg caacttctcg ttcggcgact
     1741 acttcaagaa ggatgcgatc cgcttcgcct gggatttcgt gacgggcacg ctgaagatcg
     1801 accccaagcg gctcatggtc acggtctacg cgaccgacga cgaagcgtac gacctttggc
     1861 acaagatgat cgggctgccc gccgacaaga tcgtccgcat cggcgacaac aaagcggacg
     1921 gcggctcgga taatttttgg cagatgggcg acacgggtcc gtgcggtccg tgcacggaga
     1981 tcttttacga ccacggcccg caggttgcgg gcggcccgcc gggctccgcc gatgccgacg
     2041 gcgaccgctg ggtcgagatc tggaacctcg tcttcatgca gttcgaccgc tccgccgacg
     2101 gcaagctcac gccgctgccg aagccgtccg tcgatacggg cgcgggtctc gagcgcatcg
     2161 cggccgtcat gcagggcgtc acgaacaact acgacatcga tttgttcgtc gagctgattc
     2221 gtgaggccgc tcgcttgctc ggcacgaacg actacaagaa tccgtcgttg cgcgtcatcg
     2281 cggatcacat ccgtgcatgc agctttctcg tcgtcgacgg cgtgatcccg tcgaacgaag
     2341 ggcgcggcta cgtgctgcgc cgcatcgcgc gacgcgcgat gcgccacggc tacaagttcg
     2401 gcgtgagccc cgcgtttttt cacgagctcg tgccgacgct cgcaaaggtc atgggcggtg
     2461 cctacccgga gctcgtcgcg aaacagaagt tcgtggccga ggtgctgctc aaggaaggcg
     2521 agcagttcgc acgcacgctc gagacgggca tggagatcct cgacgccgcg atcgggaagc
     2581 ttggaaataa caagatcatc gacggcaaca ccgtcttcaa gctgcacgac acctacggtt
     2641 ttcctacgga tctaacggcc gacatcgcgc gcgagcgtgg gctgacgatc gacacggaag
     2701 gctacgagcg tgcgatggac gtgcagcgca agcaatcgca ggcggcgagc cagttcggcg
     2761 tcgacttgtc ggcggcgagc gcaaagatcg atttgaagac ggagtttttg ggctacgagc
     2821 tgctcgtcag cgacgcgccc atcgtcgcgc tgctcgactc ggaaggtaac gccgtgcagg
     2881 cgctcgaagc cggcgcggcc ggcacggtcg tgctcgagcg cacgccgttc tacgccgaga
     2941 gcggcggcca ggtaggcgac tcgggagtgc tgtcgtcggg cgctgcccgg ttcgacgtcg
     3001 ccgacacaaa gaaagtcggc actgcgttcg ggcatcacgg caaggtgacg gtcggccgcg
     3061 tcgccatcgg cgatcgcgtg caagcgcaag tcgacgcgaa gcgccgcgcc tcgatcgtgc
     3121 taaaccattc ggcgacgcat ctgttacacg cggcgctgcg caaggtgctc ggcgctcatg
     3181 tccagcaaaa aggctcgctc gtcgagccgg accggctgcg cttcgacttc tcgcacttcg
     3241 agcccgtcag cgccgagcaa ctcaaggaga tcgaggcgct cgtcaacgag caggtgcgtt
     3301 tgaaccacgg cgccgacatc caggagctgc cttacgagca agcgatcgcg gccggcgcgc
     3361 tcgcgttttt cggcgacaag tacggcgaca aagtgcgcgt gttgaagctc ggcgaatttt
     3421 cgaccgagct ctgcggcggc acgcatgtcg cccgctccgg cgacatcggc ctgttcaaga
     3481 tcgtcagcga gggcggcatc gcggccggcg tccggcgtat cgaggccgtc acgggccaag
     3541 gagcgctcga cgtcgtcagc gcgaacgaag cggtgctgcg cgaggtcgcg agtctcgtca
     3601 aagcgaatcg cgacgacgtt gcggcgaagg tcacgcaact cgtcgagcgt gcgcgctcgc
     3661 tcgagcgcga gttgcaaacg ctgcgccaga agctcgcgag cggcggcggg cgcgacatcc
     3721 tgaaggaagc gaaggtcgtc aacggcatca acgtgctcgt ggccaggctc gatggcgccg
     3781 atgcgaaggc gttgcgtgac acagccgatc agctgaagag caagctcggc tccgccgtcg
     3841 tggttctcgg tgccgtcgaa ggggacaaag tttttctggt cgcgagcgtc acgcaggatc
     3901 tcgcgggccg gctcaaagca ggcgaaatca tcaagcccat tgcggagtta tgcggggggc
     3961 gcgggggcgg ccgggccgac ttcgcgcagg ctggcgggac caagcccgac aaagtcgacg
     4021 ccgctttggc gctcgtcccc gagttgatcg cggcggccta atctcctgga gtttgtctaa
     4081 atccggcact ccacaaatgt cgatactgtc agtggttttt cggctaccat gaggccgctt
     4141 cgccggttgc cccgagggaa tcgcgagagc aggggggacc gaccagaacg acaagaacag
     4201 gtcggtcagc cgcgaagtct gtaacgagtt tcgtgtggca cgcgagtgcg tcacgggcga
     4261 gttgcggcct aggggcgaaa tgccgagtcc gaaataacaa cacgctcagc gttagcgcga
     4321 gagcaacgga ccggcgggca gggcctagaa ggagttggct tatgctgatc ttgacccggc
     4381 gcgtggggga aacggtggtg atcggcaacg acgtcaccgt gaccgtactg ggggtgaagg
     4441 gcaatcaagt gcggctcggc gtgaatgccc cgcgcgaagt cgccgtccac cgcgaagaga
     4501 tcttcgaacg catcaagcgc gagcagtccg aagaacaacc gcgtccgcat ctgaacggca
     4561 ccgcgcaccc atagcggagc ccgcgcgcat cactcgagcg cgtcgcccgc acgcgggcgt
     4621 cgcgattccg tcgttccctc agtagaatta gccggcgcga atttgcttcc gcattccgga
     4681 gagatggccg agtggtcgaa ggcgcacccc tgctaagggt gtaagggcca aaagcccttc
     4741 gagggttcga atccctctct ctccgccaat tactggtttc acggcaaggc ctttatctgg
     4801 ccagccgaat tggaagtgga aaaacgagtt gcccgcgatt gcgaccaatc tgagcggcac
     4861 cctcggtcaa ctcagagcgc tcgacgatgc ggcgctcgct taataaccga tgcgacaccg
     4921 agcgctaagt cgccaagcaa aatccgttac gattccctac gcaaccgatg tgttttgtga
     4981 agccgaaaat cagccgatca accgcccgtt aatcacctcg cgcgatagcg gctagaagtt
     5041 ctcggcgagc actctgcacg gcttctgccg cggcctgttc acgaataagc tggtcgttgc
     5101 tcggctgctg ccagccttcg tcatccgggc gttctggtcg gcgacgagct ggtgagcatc
     5161 ccaaagggat cggtacctcg cgcgaagtgc ggcaatactc ataaccgtgg cattctgaca
     5221 ccacaattgc gccaaaggga tcaaggacta agggaccgag cgttttttca tgctgtgcga
     5281 atagcgc
//
LOCUS       DATLEE010000318         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1114244, whole genome shotgun sequence.
ACCESSION   DATLEE010000318 DATLEE010000000
VERSION     DATLEE010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114244"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VM692_06805"
     CDS             <1..822
                     /locus_tag="VM692_06805"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HUQ51913.1"
                     /translation="GLGYYARARNLWKAARIVVEQHAGELPTDFDALHALPGIGRSTA
                     GAILAQAHGGRWPILDGNVKRVLARYHAVVGWPGLPAVANELWRHAETHTPHERVADY
                     TQAIMDLGATLCARSRPACTVCPLPSDCAAARAGRQALYPAPRPKRERAQREVAVLVI
                     RDPDGRVLLERRPTRGIWGGLYSLPELPADDSARDWCKRVLGAAVAAERVLAKIEHAF
                     SHFDLELVPRLLELAAAPAVVNDRDDWHWCRPGATPEVGVPAPVATLLRAGLASR"
     gene            915..1193
                     /locus_tag="VM692_06810"
     CDS             915..1193
                     /locus_tag="VM692_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HUQ51914.1"
                     /translation="MSKPLQVYLEADDLARLETFAREQGWTKSQVIRTAIRALTRQRS
                     NDPVLDLSGDIDALPADMSREFDRHLNETFVAEPHATYRARRRKTVRR"
     gene            1150..1587
                     /locus_tag="VM692_06815"
     CDS             1150..1587
                     /locus_tag="VM692_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HUQ51915.1"
                     /translation="MPRTAHVAARLFVDSGAWIALRSRRDQHHAAADRAFRTAIEQRI
                     PLVTTNLILAETHRLTLFRAGREPALRALERIDASPSVTVYFATRDDHAAARRWLEQL
                     APHPVTYTDAVSFAVMEKAKCSHVLGFDGDFAVAGFELWPQAR"
     gene            1627..1896
                     /locus_tag="VM692_06820"
     CDS             1627..1896
                     /locus_tag="VM692_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HUQ51916.1"
                     /translation="MARKITCVLLGPDAEGLDRPPYPGALGQRIYENVSKLAWQRWLK
                     HQTMLINEYRLSPIEPKARKLLETEMQKFFFGEGSEAPPDFKPKQ"
     gene            1909..>2182
                     /locus_tag="VM692_06825"
     CDS             1909..>2182
                     /locus_tag="VM692_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HUQ51917.1"
                     /translation="MALDVIGVGLGRTGTLSLKVALEMLGLKPCHHMVEVWANPPSIG
                     DWLQAANGRPNWDKIYDGYRATVDYPDCHFWRELTAAYPKAKVLLTV"
BASE COUNT          352 a          748 c          728 g          354 t
ORIGIN      
        1 ggtctcggtt attacgcccg cgcgcgcaac ttgtggaaag cggcgcgaat cgtcgtagag
       61 cagcacgctg gcgaactgcc gacggacttc gacgcgttgc acgcgctgcc cggcatcggc
      121 cgctcgaccg ccggcgcgat cctcgcgcaa gcgcacggcg gcagatggcc gattctcgac
      181 ggcaacgtga agcgcgtgct cgcgcgctat cacgccgtcg tgggctggcc tggcctgccg
      241 gccgtcgcaa acgagctctg gcggcacgcc gagacgcata cgccgcacga acgcgtcgcc
      301 gactacacgc aagcgatcat ggatctcggt gcaacgctgt gcgcgcgctc gcggcccgcc
      361 tgcacggtgt gcccgctccc gagcgattgc gcggccgcgc gcgccggcag gcaggcgctc
      421 tatcccgcgc cgcggccgaa gcgcgagcgc gcacagcgcg aggtcgccgt gctcgtcatt
      481 cgcgaccccg atgggcgcgt gctgctcgag cggcggccga cgcgcgggat ctggggcggc
      541 ctctacagct tgccggagct gccggccgac gattcggcgc gcgactggtg caagcgcgtg
      601 ctcggcgccg ccgtcgcggc cgagcgcgtg ctcgcaaaga tcgagcacgc gttctcgcac
      661 ttcgacctcg agctcgtgcc gcgcttgctc gagctcgcgg ccgcgcccgc cgtcgtcaac
      721 gaccgcgacg actggcactg gtgccggccg ggcgcgacgc ccgaggtcgg cgtgcccgcg
      781 cccgtcgcca cgctgctgcg tgcagggctt gcgtctcggt agctcgtcgc cggaacagaa
      841 aagactgcgc ccacgacctt tgcgtctcat gagcgctttg acctcgtgct catgagcgcg
      901 tagcgtatgc ggtcatgagc aagccgctgc aggtctatct cgaggctgac gacctggccc
      961 gcctggagac cttcgcgcgc gagcaaggct ggaccaaatc gcaagtcatt cgtacggcga
     1021 ttcgcgcgct gacgcgtcaa cgttccaacg atcccgtgct cgatctttcg ggcgatatcg
     1081 acgcgctacc ggcggacatg agccgggagt tcgatcgcca cctcaacgag accttcgtcg
     1141 ccgagccgca tgccacgtac cgcgcacgtc gccgcaagac tgttcgtcga tagcggggca
     1201 tggatcgcgc tgcgcagccg gcgcgaccag caccacgctg cggcggaccg cgcgttccgc
     1261 acggcgatcg agcagcggat accgctcgtg acgactaact tgattctcgc cgagacgcat
     1321 cggctgacgt tgttccgtgc aggccgagag cctgctttgc gggcgctcga gcgaatcgac
     1381 gcgagcccga gcgtcaccgt ttatttcgcc acgcgtgacg atcacgcggc cgcgcgccgc
     1441 tggctcgagc agctcgcgcc gcacccggtg acttacacgg acgcggtgag tttcgcggtg
     1501 atggagaagg cgaagtgcag ccacgtcctc ggtttcgacg gagacttcgc cgttgcgggt
     1561 ttcgagcttt ggccgcaggc gcgatagctc ttctcagcgg cggtctcgtc gcggcatagt
     1621 gcgcgcatgg caagaaagat cacgtgtgtc ctgctcggcc ccgatgccga gggcctcgac
     1681 cggccgccgt accccggcgc gctcggccag cgcatttacg agaacgtgtc gaagctcgcg
     1741 tggcaacgct ggctcaagca tcagacgatg ctgatcaacg agtatcgtct gtcgccgatc
     1801 gagccgaagg cgcgcaagct cctcgagacc gagatgcaga agttcttctt cggcgaaggc
     1861 tccgaagcgc cgccggactt caagcccaag caataaggaa taacgcgcat ggctctcgac
     1921 gtcatcggcg tcggcctcgg ccgcacggga acgttgtcgt tgaaggtcgc cctcgagatg
     1981 cttgggctca aaccctgcca tcacatggtc gaggtctggg cgaacccgcc ctcgatcggc
     2041 gattggctgc aagccgcgaa cggccgcccg aactgggaca agatctacga cggctatcga
     2101 gcgaccgtcg attacccgga ctgccatttt tggcgcgagc tgacggccgc gtatccgaag
     2161 gccaaagtgc tgctgacggt gc
//
LOCUS       DATLEE010000319         2237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2604821, whole genome shotgun sequence.
ACCESSION   DATLEE010000319 DATLEE010000000
VERSION     DATLEE010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2604821"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /gene="secD"
                     /locus_tag="VM692_06830"
     CDS             <1..704
                     /gene="secD"
                     /locus_tag="VM692_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HUQ51918.1"
                     /translation="GVFSNRFRISGLSVFESQNLSLLLRSGSLAAPIVPIEERTIGPS
                     LGRDNIDRGIEVVLIGYLLVVIFIGLWYRVFGMLANVALFVNVIMIVAVMSLFDATLT
                     LPGIAGIVLTVGMSVDANVLIFERIREELGNGNTPQASIRAGWDKAYTTIVDSNLTTV
                     IAAIVLFMYGTGPIKGFAVTLTIGIATSMFTAITVTRALVNFVYGSRSDLKMLSIGGG
                     KVANPALPTVTTGRA"
     gene            complement(701..1486)
                     /locus_tag="VM692_06835"
     CDS             complement(701..1486)
                     /locus_tag="VM692_06835"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HUQ51919.1"
                     /translation="MVNIAVRAARRAGEIMIRQMNQLEALKVVEKSRNEFVSQVDQAA
                     EAAIIEVIKDHYPDHAILAEESGSAGEHEYQWIIDPLDGTTNYLHGFPQFSVSIGVAR
                     KGELEHGVVYDPLRQEIFTASRGQGAQLDGRRIRVSKRTTLQQSLIGTGFPYRANLEH
                     IERYLKMLRAVMLESAGVRRPGSAALDLCYVAAGRIDAFFELGLSKWDIAAGALIIRE
                     AGGRVSDFHGGDGYLDSGNVVAGNPKTYAALSKLLAPFSAGVP"
     gene            1554..>2237
                     /locus_tag="VM692_06840"
     CDS             1554..>2237
                     /locus_tag="VM692_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HUQ51920.1"
                     /translation="MDPIPLHIPVRIVLVDTSHPGNIGATARAMKTMGLRDLALVRPR
                     HFPHDDATARAAGANDILLAARVHEDLASAIADCGWVVGASARLRTIALPIVDPREAA
                     LTIWQRLGANRVAVLMGPEQSGLTNDDLARCQQLVHIPTNPEFSSLNLAMAVQVLCYE
                     LRMAMPDRPVPEGPVSDSRLATAAELEGLHEHLERLLTESEFLHPAHQKQVKLKLRRI
                     FHKATLEENE"
BASE COUNT          378 a          714 c          730 g          415 t
ORIGIN      
        1 aaggcgtgtt ctcgaaccgt ttcaggatca gcggcctcag cgtcttcgaa tcgcagaatc
       61 tttcgttgct gttgcgctcc gggtcgctgg ccgcacccat cgtgccgatc gaagagcgca
      121 cgatcggtcc gagcttgggc cgagacaaca tcgaccgcgg catcgaagtc gtgctgatcg
      181 gctatctgct cgtcgtgatc ttcatcggcc tctggtaccg cgtgtttggg atgctggcga
      241 acgtggcgtt gttcgtgaac gtcatcatga tcgtcgcggt catgtcgttg ttcgatgcga
      301 cgctgacgct accgggtatc gcgggcattg tgctcacggt cggcatgtcc gtcgacgcga
      361 acgtgctgat tttcgagcga attcgtgaag agctcggcaa cggcaacacg ccgcaggcga
      421 gcatccgcgc aggctgggac aaggcttaca cgacgatcgt cgactcgaac ctgacgacgg
      481 tcatcgcggc catcgtgctg ttcatgtacg gcaccgggcc gatcaaaggg ttcgcggtga
      541 cgctgacgat cggtattgcg acgtcgatgt tcacggccat caccgtgacg cgcgcgctcg
      601 tcaacttcgt ctacggctcg cgctccgact tgaagatgct gtcgatcggc ggcggcaagg
      661 tcgcgaatcc ggcgctgccg acggtgacga cagggcgcgc ttaaggcact ccggccgaga
      721 acggcgcgag cagcttcgat agcgcggcgt aagtcttcgg attgcccgcg acgacgttgc
      781 cgctgtcgag ataaccgtcg ccgccatgga agtcgctgac gcggccgccg gcttcgcgga
      841 tgatcagcgc gccggccgcg atgtcccact tgctgagccc gagctcgaag aacgcgtcga
      901 tgcgtcccgc cgcgacatag caaaggtcga gcgcggcgga gccgggtcgc cgcaccccgg
      961 ccgattcgag catgacggca cgcagcatct tcaagtagcg ctcgatgtgc tcgagattgg
     1021 cccgatacgg aaagccggtc ccgatcaacg actgctgtag cgtcgtgcgc ttgctgacgc
     1081 ggatgcgccg gccgtcgagc tgcgcgccct ggccgcggct cgccgtgaag atctcttgtc
     1141 gcagcgggtc gtagacgacg ccgtgctcga gctcgccttt acgcgcgacg ccgatcgaca
     1201 cggagaattg cgggaagccg tgcagatagt tcgtcgtgcc gtcgagcggg tcgatgatcc
     1261 attgatattc gtgctccccg gccgagccgc tctcctcggc gagaatggca tggtcggggt
     1321 aatggtcctt gatgacctcg atgatcgcgg cttcggcggc ttggtcgact tgcgagacga
     1381 actcgttgcg gctcttctcg acgaccttca gcgcttcgag ctggttcatc tggcgaatca
     1441 tgatttcgcc ggcgcgccgc gcagcgcgaa ctgcaatgtt gaccatgccg tgcattgggc
     1501 gcgcagcgta gaataagcgc cggtccgaag gcaaacgcgc gcgttggcgc cgcgtggatc
     1561 cgatcccctt gcacatcccc gttcgcatcg tcctcgtcga cacctcgcac cccggcaaca
     1621 tcggcgccac ggcgcgcgcg atgaagacga tgggactccg cgacctcgcg ctcgtgcgcc
     1681 ccaggcattt tccgcatgac gatgcgacgg cgcgcgcggc cggcgccaac gacattctcc
     1741 tcgctgcacg tgtccacgaa gaccttgcga gtgcgatcgc ggattgcggc tgggtcgtcg
     1801 gcgcgagcgc gcgcctgcgc acgatcgctt taccgatcgt cgatccgcgc gaggcggcgc
     1861 tgacgatctg gcagcgcttg ggcgcgaatc gcgtcgccgt cctgatgggg cccgagcaat
     1921 cggggctcac gaacgacgat ctcgcgcgct gccagcaact cgtacacatt ccaacgaatc
     1981 cggagttcag ctcgctgaat cttgcgatgg ccgtgcaggt gctctgctac gagctgcgca
     2041 tggcgatgcc cgatcggccg gtcccggaag gcccagtcag cgacagccgt ctcgctacgg
     2101 ccgccgagct cgaagggctg cacgagcatc tcgagcggct gctgacggag tccgagttcc
     2161 tgcacccggc gcaccagaag caggtcaagc tgaagctgcg ccgaattttt cacaaagcga
     2221 cgctcgagga gaacgag
//
LOCUS       DATLEE010000320         2230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6147450, whole genome shotgun sequence.
ACCESSION   DATLEE010000320 DATLEE010000000
VERSION     DATLEE010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6147450"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VM692_06845"
     CDS             complement(<1..497)
                     /locus_tag="VM692_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51921.1"
                     /translation="MFFATDALVIGLRAITFVALFQAAGAALFLALYDPQLERACAER
                     LRTVARVAALVALIAAVAHYVLTPARMAGDFGSTFDPALESLLLRSNSGAAHIVRVVG
                     LAILLLSLDRASKLNTCAACGGAALALLSFVLMGHTVIHEQRWILAPLLLVHVVVVAV
                     WLGALA"
     gene            complement(507..860)
                     /locus_tag="VM692_06850"
     CDS             complement(507..860)
                     /locus_tag="VM692_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HUQ51922.1"
                     /translation="MPRLITLVLGLALAFAAQAHTALKSSAPAAEASIAAPKTIDLTF
                     AGDVRLTSLTLTDAGGTAKHLDAVPTAVASTFSLAVHEPLTPGAYKVVWRAVGGDTHI
                     VSGEFAFTVVATPTR"
     gene            complement(916..1890)
                     /locus_tag="VM692_06855"
     CDS             complement(916..1890)
                     /locus_tag="VM692_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51923.1"
                     /translation="MRIYLVVAGACVGAALLADAAHAQSSTFCSAAGAYMHEVAVRRA
                     SGATRDDAVQQLTSEFEAFAGDQADLAARRRVLQVGPSLAAFAVDLDGLRPATVQSVG
                     QAYCAERGGYVSLAPSSTVSRQFVAAGLECESGSAEALDSCVAQGIQRSEVRVARETS
                     LKREGINKIQPFYEFAASYGGDAIGTIIFVGGNTQEVDSGNGLSAGGGFLHRITESFG
                     IKYTASYKVSFSAASNADVMKSVLPIDIVPYYQTGDHRFGVGLSHHLSPKVDWDWLMP
                     ATDFDDATGVIVEYAWKRLSFSYTDIDYESGPASIDASHFSVKYTSRH"
BASE COUNT          405 a          740 c          735 g          350 t
ORIGIN      
        1 gccagcgcgc cgagccacac ggcgacgacg acgacgtgga ccaacaacag cggcgccaaa
       61 atccagcgct gctcgtgaat cacggtgtga cccatcagca cgaacgacag caacgcgagg
      121 gcggcgccgc cgcatgcagc acacgtgttc agcttgctgg cgcggtcgag gctcagcagc
      181 aagattgcaa gaccgacgac gcgcacgatg tgcgctgcgc ccgagttcga gcgcaacagc
      241 aatgactcga gcgccggatc gaacgtgctg ccgaaatccc cggccatgcg cgccggagtc
      301 aagacgtagt gtgcgacggc cgcgatcagc gcaacgagcg cggcgacgcg cgccacagtg
      361 cgcaaccgct ccgcgcaagc gcgctcgagc tgcgggtcat agagggcgag aaacagagcc
      421 gcgccggcgg cttggaacag cgcgacgaag gtgatcgccc gcaggcctat gacgagcgca
      481 tcggtggcaa agaacacgag cgctcgtcag cgcgtcggcg tcgcgaccac cgtgaatgca
      541 aactctccgg acacgatgtg cgtgtcgccg ccgaccgcgc gccagacgac cttgtaagca
      601 cccggtgtca gcggctcgtg cacggccaac gagaacgtgc tcgcgacggc cgtcggcacg
      661 gcgtcgaggt gcttcgcggt accgccggca tcggtcaacg tcagcgacgt caggcgcacg
      721 tcgccggcga acgtaaggtc gatggtcttc ggcgcggcaa tggaagcctc cgcggccgga
      781 gcggatgact tcagcgccgt atgcgcctga gcggcgaagg cgagcgccaa acccaggacg
      841 agtgtgatca gacgtggcat cgcaggtctc ctcgggcgcg attctactcg ccctgaaagc
      901 gccacgcctg cgtgactaat gccggctcgt gtatttcacg ctgaagtggc tcgcgtcgat
      961 cgaggcgggc ccgctctcgt agtcgatgtc cgtgtacgag aacgacaggc gcttccaagc
     1021 gtattcgacg atgacaccgg tcgcgtcgtc gaagtccgtg gccggcatga gccagtccca
     1081 atcgaccttc ggcgacagat ggtggctcag cccgacgccg aagcggtggt cgcccgtttg
     1141 atagtacgga acgatatcga tcggcagcac cgacttcatg acatcggcgt tgctggcggc
     1201 cgagaacgaa accttgtacg acgccgtgta cttgatcccg aacgattccg tgatgcggtg
     1261 caggaagccc ccgccggcgc tcaagccgtt acccgagtcg acctcttgcg tgttgccgcc
     1321 gacgaagatg atcgtgccga tcgcgtcgcc gccataggag gccgcgaact cgtagaacgg
     1381 ttgaatcttg ttgatgccct ctctcttcag cgacgtctcg cgcgccactc tgacttcgct
     1441 tcgctgaatg ccttgcgcga cacacgagtc gagcgcctcc gccgacccgc tctcgcactc
     1501 gaggccggcc gcgacgaact gccgcgacac cgtgctcgag ggcgccaagc tcacgtagcc
     1561 gccgcgctcg gcgcaatacg cctggccgac gctttgcacg gtggctggcc gaagaccatc
     1621 gagatcgacg gcgaacgccg ctaacgaagg cccaacttgc agcacgcgcc ggcgcgcggc
     1681 gaggtccgct tgatcccctg cgaacgcctc gaactcggac gttagctgtt gcacggcgtc
     1741 atctctcgtg gcgccgcttg cgcgtcgaac cgcgacctcg tgcatgtagg cacccgcggc
     1801 cgagcaaaac gtcgagctct gagcgtgcgc cgcatcggcg agcagcgctg cgccgacaca
     1861 agcgccggcg acgacgaggt aaatacgcat ggcaagcatc cgctgattcg atggggcaca
     1921 gcgtatcggc agcggcatga caccggtttg acgccagtcg catttttggc agggcttttc
     1981 gaggtgccga accggcctgg cttgggcggc ccgcgagacc ggtggtatgg tggccgccga
     2041 tgctctgggg gacctctgat gccgactgac gttctggtgc aatcgcggcg caatttcctg
     2101 cgtttcttgg ccgagagccc gctgttctgc ggcctcgggg gcctcggcgg cacgctgctc
     2161 gccggcgcgg cgagcgcgca agacgactcg gtgctctcgg gctcgctcgc gttcacggac
     2221 ctcattacga
//
LOCUS       DATLEE010000321         8964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6894547, whole genome shotgun sequence.
ACCESSION   DATLEE010000321 DATLEE010000000
VERSION     DATLEE010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6894547"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..118
                     /locus_tag="VM692_06860"
                     /pseudo
     CDS             1..118
                     /locus_tag="VM692_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
     gene            170..1762
                     /gene="ctaD"
                     /locus_tag="VM692_06865"
     CDS             170..1762
                     /gene="ctaD"
                     /locus_tag="VM692_06865"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HUQ51924.1"
                     /translation="MSSTAIDTHGHHGEHHSHGFVAWCLRWLTTTNHKEIGTLYLVWS
                     LLMLFVGGGMAMLIRLELFQPGLQFLQPQFFNSMTTLHALIMVFAMIMPAFAGFANWM
                     IPLMIGAPDMALPRLNNWSFWLLPFAVGLLILTLFLPGGAPAGGWTLYPPLVLQTGDA
                     FPFAVFAVHLLGVSSILAAINIIVTIMNLRAPGMSLLKMPMFVWTWLITAYLLVLAMP
                     VLAGAVTMLLTDRFFGTSFFSAAGGGDPVLFQHIFWFFGHPEVYIIILPAFGIVSEII
                     PTFARKPLFGYAAMVYATASIAFLSLVVWAHHMFTAGMPLAGQLFFMLSTMIIAVPTG
                     VKVFNWTATLWKGSLTFETPMLFALAFLFLFTLGGFSGLMLSIVPADIQYHDTYFVVA
                     HFHYVLVPGALFGAFAGAYYWLPKWTGHMYDETLGKVHFWLTAIFTNVTFFPQHFLGL
                     AGMPRRIPDYAPQFADFNALSSIGAFGLGLSQLLFVYVIVKCVRGGPKATAEVWDKPA
                     GLEWTVPSPAPHHTFDEPPVIK"
     gene            1774..1938
                     /locus_tag="VM692_06870"
     CDS             1774..1938
                     /locus_tag="VM692_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51925.1"
                     /translation="MSQVEQRMEQLPGAVAGTDAARAAKVRRNAWLLGGLAAFFYVGY
                     IVWMIVRSGG"
     gene            1946..2464
                     /locus_tag="VM692_06875"
     CDS             1946..2464
                     /locus_tag="VM692_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007469510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HUQ51926.1"
                     /translation="MSAAPDRYLVLKVAALGLAMFGFGFALVPLYDVFCDITGFGGKT
                     AAAAVVIAETPDVSRTVRVEFLASVPRGVPFTLEPEVSHLDVHPGQMYETHFRAQNLT
                     GSPLTAQAVPSVAPGAAARYFNKAECFCFTSQSFQPHETLDLKLAFMVATELPAHVDT
                     LSLSYTYFTASN"
     gene            2480..3367
                     /locus_tag="VM692_06880"
     CDS             2480..3367
                     /locus_tag="VM692_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HUQ51927.1"
                     /translation="MAHATPSHTAAPDASRYYVPHGTRWPILGSIGLFTMMVGAALWL
                     NAFGAGPWIVLTGFAVLLVMLFGWFGTVIGESEGGLYNLKVDASFRWAMSWFIFSEVM
                     FFAVFFGTLFYARVLVVPWLSGAGDNFFTNFLLWPGFENTWPSTGPRGEDVERMGAWG
                     LPAVNTAILLLSGVTITIAHHALRAGHRTQLIIWLAATFLLGFLFLGFQAEEYIHAYT
                     ELNLRLDTGIYGGTFFMLTGFHGLHVTLGAIMLLIIWLRVLRGHFKPDRHFAFEAVAW
                     YWHFVDVVWLGLFFFVYWI"
     gene            complement(3364..3561)
                     /locus_tag="VM692_06885"
     CDS             complement(3364..3561)
                     /locus_tag="VM692_06885"
                     /inference="COORDINATES: protein motif:HMM:NF022587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2909 family protein"
                     /protein_id="HUQ51928.1"
                     /translation="MIAAILFVAILATLGVALLALFRRGDNSQTLLRALTIRVSLSVL
                     FFLLLMLGWYLGILEPHGLGG"
     gene            3601..4359
                     /locus_tag="VM692_06890"
     CDS             3601..4359
                     /locus_tag="VM692_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HUQ51929.1"
                     /translation="MITATGTARTYVAIAGLLLLGLLFASLGTWQLHRAEASRATRAQ
                     FAGGAADSALAELPLELDDAARFRRAEVRGEYVGRPQFLLDNMLHDGVAGYQVLTALR
                     IPGRRERVLVNRGWVPAGGDRRALPDIAVGSETRLVAGRLEHLPRPGMRLGANVSGDA
                     VADAVVVLQYPVAAELEQRLGEPVYDYELLLDAAAADGYVREWQAPGLDPERHLAYAG
                     QWWALAVGAVAAAMVMAYRTIRGSPQRGALRRKP"
     gene            4356..4904
                     /locus_tag="VM692_06895"
     CDS             4356..4904
                     /locus_tag="VM692_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51930.1"
                     /translation="MKHKSLAPWLVGLVCFGPFAVALFIYYGPLGLSWLPQLPGSREL
                     LRDPVAVPPEWRTDVEPDAPHAWQLIYARMTSCEQQCAQHLGRLLQVQLALGRDQGRV
                     TRAVWHVGSLPQLDDPQLAAHDLDDAAGRALVAALGAERLQEGRVYVADPRGSVVLSY
                     PPDVEQKELLRDLKRLLAGSDS"
     gene            4901..5875
                     /locus_tag="VM692_06900"
     CDS             4901..5875
                     /locus_tag="VM692_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HUQ51931.1"
                     /translation="MNVWFVRLARIGTVVALAVVMLGAYVRLSDAGLGCPDWPGCYGR
                     LVVPHPATTAEELGSEFTRPLEAGKAWREMIHRYLASTLGLICVALAAIAWRNRRDPK
                     QPWRAPLALVALVIFQGLLGMWTVTLLLKPIVVVAHLLGGFATLALLASLGRWRATRF
                     PQPTAAVRLLGVAAASALVLQISLGGWTSANYAALACPDFPTCQTQWWPAIADFDEGF
                     VLWRGIGIDYEGGVLDHPARVAVHFTHRLGAVLAALLIALLGIGLVRAAETRLDGSAV
                     LAALLLQLCLGVSIVWFGVPLPVAVLHNGVAALLLLTVINANQRIRQR"
     gene            5847..6758
                     /gene="cyoE"
                     /locus_tag="VM692_06905"
     CDS             5847..6758
                     /gene="cyoE"
                     /locus_tag="VM692_06905"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011576923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HUQ51932.1"
                     /translation="MLTNELGNVSSAGAHWRDYYVLTKPGVVQLLVFTAIVGMFLATP
                     GMVPWEILIAASVGIGLAAAAGAVVNQVLDQRIDAQMARTRNRPLPQGRVTERDALAF
                     ALGLALGGIVVLVLFVNPLTAALTFLSMIGYAVVYTVYLKRATPQNIVIGGAAGAAPP
                     VLGWAAVTNSVDPHALLLFLIIFTWTPPHFWALAIARRTDYERADIPMLPVTHGEELT
                     KSFVIYYTILLLLVTVLPSITGMTGLFYTLGALLLGGGFLYYAFILKFAPRQDSPMAT
                     FRFSIWYLMALFSFLLIDHYIPALWPA"
     gene            6802..8958
                     /gene="uvrD"
                     /locus_tag="VM692_06910"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             6802..8958
                     /gene="uvrD"
                     /locus_tag="VM692_06910"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HUQ51933.1"
                     /translation="MDVTDIIDPLNEAQREAVAAADRPTLVLAGAGSGKTRVLVHRIA
                     WVIRVNGVAAQHVLAVTFTNKAAAEMRDRVESLLGVPTRNLWIGTFHGLSHRLLRIHW
                     REAGLAEGFQIMDSEDQQRMIRRMLRALELDENVWVPKEIQWFINAQKDEGLRPKDLR
                     DEGNAGRRQMIDLYERYERVCAAASVIDFGELLLRSLELLRDNLELQRQYQHRFQHVL
                     VDEFQDTNAIQYRWLRLLAGPAGTPFVVGDDDQSIYRWRGARVENLYRFQQDYPGTQL
                     VRLEQNYRSTANILNAANAVIANNASRLGKTLWTEGEAGAPIKVYAAFNDRDEAEFVV
                     NRIQDHVNQGGKRSEAAVLYRSNAQSRVLEEGLLAAGMPYRVYGGLRFFERQEIKDAL
                     AYLRLVMNRDDDPSFERVVNTPPRGIGARTVEAVREQARAAGTSLWRAAATVIADNRL
                     AKRANESLWAFLSLVESLDKDTRGDALHDQVARVIERSGLMAHYGKNPDAGGEARVEN
                     LEELVNAAREIMRSDEGELPPLAQFLAFAVLESGAGQAEAYQDSVQLMTLHSAKGLEF
                     DLLFLCGMEDGLFPHQRSLTDPAGLEEERRLCYVGITRAKRELYLTHAEQRRLHGEMH
                     YSVPSRFLREVPAELMEEVRPQMVVARPIFRRSDVSEPVFGSALKLGQRVRHGKFGEG
                     IVLRRYGSGAHTLVEVNFESSGMKTLVLTQANLELM"
BASE COUNT         1331 a         2997 c         2934 g         1702 t
ORIGIN      
        1 ccagtgcgcc gagctctgcg gcatggatca cgcgttcatg ccgatcgtcg ttcgcgttac
       61 gagcaaagac gactacgact cgtggctgag ctcgcggcgt gagcaggtta gctcgttggc
      121 cgcgcactag caccgctcgg acgcaaagac ggaacaagga agatttggca tgagcagcac
      181 tgcaatcgac acccacggcc accacgggga gcaccactcg cacggcttcg tcgcgtggtg
      241 tctgcgttgg ctcacgacga cgaatcacaa agaaatcggc acgctctatc tcgtctggag
      301 cctgctgatg ctgttcgtcg gcggcggcat ggcgatgttg attcgcctcg agctgttcca
      361 gccgggtctg cagttcttgc agccgcagtt cttcaactcc atgacgacgc tgcacgcgtt
      421 gatcatggtg ttcgcgatga tcatgccggc gttcgcgggc ttcgcgaatt ggatgatccc
      481 gctgatgatc ggcgccccgg acatggcatt gccgcggctc aacaactggt cgttctggct
      541 gctgccgttc gccgtcggct tgctgatcct gacgttgttc ctgcccggcg gcgcgcccgc
      601 cggcggctgg acgctgtatc cgccgctcgt attgcagacg ggtgacgcgt tcccgttcgc
      661 ggtgttcgcc gtgcacttgc tcggcgtctc gtcgatcttg gcggcgatca acatcatcgt
      721 cacgatcatg aacctgcgcg cgccgggcat gtcgctgctc aagatgccga tgttcgtctg
      781 gacgtggctc atcacggcct acctgctcgt gctcgcgatg cccgtgctgg ccggtgccgt
      841 gacgatgttg ctgacggacc gcttcttcgg cacgagcttc ttctcggctg cgggcggcgg
      901 tgaccccgtg ctgttccagc acatcttctg gttcttcggt caccccgagg tctacatcat
      961 catcctgccg gcgttcggca tcgtctcgga gatcatcccg acgttcgcgc gcaagccgct
     1021 gttcggctac gcggcgatgg tctacgcgac ggcgagcatc gcgttcctgt cgctcgtcgt
     1081 gtgggcgcac cacatgttca cggccggcat gccgctcgcg ggccagctgt tcttcatgct
     1141 gagcacgatg atcatcgccg ttccgaccgg cgtgaaggtg ttcaactgga ccgcgacgct
     1201 ctggaaaggc tcgttgacgt tcgagacgcc gatgctgttc gcgctggcgt tcttgttcct
     1261 gttcacgctc ggcggtttct cgggcctcat gctctcgatc gtgccggccg acatccagta
     1321 ccacgacacg tatttcgtcg tcgcgcattt ccactacgtg ctggttcccg gcgcgttgtt
     1381 cggcgcgttc gccggcgcgt attactggct gccgaagtgg accgggcaca tgtacgacga
     1441 gacgctcggc aaggtgcact tctggctgac ggcgatcttc acgaacgtga cgttcttccc
     1501 gcagcacttc ttgggtttgg ccggcatgcc gcgccgcatc ccggattacg cgccccagtt
     1561 cgccgacttc aacgcgttgt cgagcatcgg tgcgttcggc ctcggcctgt cgcagctcct
     1621 gttcgtctac gtgatcgtca agtgcgtccg cggcgggccg aaggccacgg cggaggtctg
     1681 ggacaagccg gccggtctcg agtggaccgt gccgtcgccg gcgccgcatc acacgttcga
     1741 cgagccgccg gtcatcaaat gacgaggaca gggatgtccc aagtagagca acgcatggag
     1801 cagttgccgg gagcagttgc cggcacggac gctgcgcgcg ccgccaaagt gcgccgcaac
     1861 gcgtggctgc ttggcggcct cgccgcgttt ttctacgttg gctacatcgt gtggatgatc
     1921 gtgcggtccg gcgggtgaga ttgcgatgag cgcggcaccc gatcggtatc tggttttgaa
     1981 agtcgcggcc ttaggcctcg cgatgttcgg gttcggcttc gcgctcgtgc cgctctacga
     2041 cgtgttctgc gatatcacgg gcttcggcgg caaaaccgcc gccgccgcgg tcgtgatcgc
     2101 cgagacaccc gacgtgtctc gcaccgtgcg cgtcgagttc ctcgcgtcgg tgccgcgcgg
     2161 cgtgccgttc acgctcgagc ccgaggtcag ccacctggac gtgcacccgg ggcagatgta
     2221 cgagacgcac ttccgggcac agaacctcac ggggtcgccg ttgacggcgc aggccgtgcc
     2281 gagcgtcgcg ccgggcgccg ctgcacgcta tttcaacaag gccgagtgct tctgcttcac
     2341 gagccagagc tttcagccgc acgaaacgct cgacctcaag ctcgcgttca tggtcgcgac
     2401 ggagttgccc gcgcacgtcg acaccctgtc gctctcgtat acgtatttca ccgcgtcgaa
     2461 ctgaaggact agagcagtca tggcacatgc cacgccgagc cacaccgccg cacccgatgc
     2521 gagccgctac tacgtgccgc acggcacgcg ctggccgatt ctcggctcga tcggcttgtt
     2581 cacgatgatg gtgggtgccg cgctgtggct gaatgcgttc ggcgccggcc cgtggatcgt
     2641 cttgacgggc ttcgccgtgc tgctcgtcat gctgttcggc tggttcggca ccgtcatcgg
     2701 cgagagcgag ggcggtctct acaacttgaa ggtcgacgcc tcgttccgct gggcgatgag
     2761 ctggttcatt ttctcggaag tcatgttctt cgccgtgttc ttcggcacct tgttctatgc
     2821 gcgcgtgctg gtcgtccctt ggctctccgg cgcgggcgac aacttcttca cgaacttcct
     2881 actctggccg ggcttcgaga acacttggcc gtcgacgggg ccgaggggcg aggacgtcga
     2941 gcgcatgggc gcgtgggggt tgccggccgt caacacggcg atcctgctgc tttcgggcgt
     3001 cacgatcacg atcgcgcacc acgcgttgcg cgccggtcat cgcacgcagc tgatcatttg
     3061 gcttgcggcg acgttcctgc tcggcttctt gttccttggc tttcaagccg aggaatacat
     3121 tcacgcctat accgagctga acttgcgcct cgacacgggc atctacggcg gcacgttctt
     3181 catgctgacg ggcttccacg gcctgcacgt gacgctcggc gccatcatgc tgttgatcat
     3241 ctggctgcgc gtgttacgcg gccacttcaa gcccgatcgg cacttcgcgt tcgaggccgt
     3301 ggcctggtac tggcacttcg tcgacgtcgt ctggcttgga ttgttcttct tcgtctactg
     3361 gatctagccg ccgagcccgt ggggctcgag gatgccgaga taccacccga gcatcagcag
     3421 caggaagaac agcaccgaca agctcacgcg gatcgtcaac gcacgcagca gcgtttgcga
     3481 gttgtcgccg cggcggaaca aggccagcaa cgcgacgcct aaggtcgcga ggatggcaac
     3541 gaagagtatg gctgcgatca cgactgctgc cgaggctgct cgagacggcg gcattctaac
     3601 gtgattaccg cgaccggcac ggcgcgcacg tacgtcgcga tcgcgggtct cttgctgctc
     3661 gggttgctgt tcgcgtcgct cggcacctgg cagctgcacc gcgcggaggc gagccgcgcc
     3721 acgcgcgcgc agttcgcggg cggcgctgcc gacagcgcgc tcgccgagct gcccctcgag
     3781 ctcgacgacg cagcccgttt tcgccgcgcc gaggtccgcg gcgagtacgt cggccgcccg
     3841 cagttcttgc tcgacaacat gctgcacgac ggcgtggccg gctatcaggt cctgacggca
     3901 ctgcggatcc cgggccggcg cgagcgcgtg ctcgtgaatc gcggctgggt gccggcgggc
     3961 ggcgatcgac gcgcattgcc cgacatcgcg gtcggcagcg agacgcgcct cgtcgcgggg
     4021 cggctcgagc acctgccgcg gcccggtatg cggctcggtg ccaacgtgag cggcgacgcc
     4081 gttgctgacg cggtcgtcgt cctgcaatac cccgtcgccg cggagctcga gcagcggctc
     4141 ggcgagcccg tctacgacta cgagctgttg ctcgacgcgg ccgcagcgga cggttacgtt
     4201 cgggaatggc aagcaccggg cctggacccg gagcgccatc tggcttacgc cgggcaatgg
     4261 tgggcgttgg cggtcggtgc cgtcgccgcg gcgatggtca tggcttatag aacgattcgt
     4321 gggagtcccc aaaggggggc actgaggcgc aagccgtgaa acataaatcg ctggccccgt
     4381 ggctcgtggg actcgtgtgc ttcggcccgt tcgccgtcgc gctattcatt tattacgggc
     4441 cgctgggcct gtcatggctg ccgcagctgc ccgggagccg cgagctgctg cgcgaccccg
     4501 tcgccgtgcc gcccgagtgg cgcaccgacg tggagccgga cgcgcctcac gcgtggcaat
     4561 tgatctatgc aagaatgacc tcgtgcgagc agcaatgcgc gcaacacctc ggccggctgc
     4621 tccaagtgca attggctctg ggtcgcgacc aaggtcgcgt cacaagggcc gtttggcacg
     4681 tcggtagcct tccgcagctc gacgatccgc agctcgccgc acacgatctc gacgatgcgg
     4741 cggggcgcgc gctggtcgct gcgctcggtg ccgaacgctt gcaagaaggc cgagtgtacg
     4801 tggccgatcc gcgcgggtcc gtcgtcctga gctacccgcc ggatgtcgaa caaaaagaac
     4861 tattgcgcga cctcaaacgg ttgctggccg ggtccgattc atgaacgtgt ggtttgtccg
     4921 cctcgctcgc atcggcacgg tcgtggcgct cgccgtcgtc atgctcgggg cgtacgtgcg
     4981 cttgagcgat gcgggcctcg gttgccccga ttggccgggc tgttacggcc ggctcgtcgt
     5041 gccccacccg gccacgacgg ccgaggagct cggcagcgag ttcacgcggc cgctcgaggc
     5101 cggcaaggcg tggcgcgaga tgatccaccg ctatctcgcg agcacgctgg gcctgatctg
     5161 cgtcgcgctc gccgcgatcg cgtggcgcaa tcggcgcgat ccgaagcagc cgtggcgggc
     5221 gccgctcgcg ctcgtcgcgc tcgtgatctt ccagggcctg ctcggcatgt ggacggtcac
     5281 gctgctgctg aagccgattg tcgtcgtcgc ccatctgctc ggcggcttcg cgacgctggc
     5341 cctgctcgct tcgctgggcc gctggcgagc gacacgcttt ccgcagccga cggccgccgt
     5401 ccgcttgctc ggcgtcgcgg ccgcgagcgc gctcgtcctg cagatttcgc tcggcggctg
     5461 gacgagcgcc aattacgccg cgctcgcgtg ccccgacttc ccgacgtgcc agacgcaatg
     5521 gtggcccgcg atcgccgatt tcgacgaggg gttcgtgctc tggcgcggca tcggcatcga
     5581 ttacgaaggc ggcgtgctcg atcacccggc tcgcgtcgcc gtgcacttca cgcaccggtt
     5641 gggcgccgtc ctcgcggcgc tattgattgc gctgctcggc atcgggctcg tgcgcgcagc
     5701 cgaaacgcgg ctcgacggca gcgcagttct ggccgcgctc ctgctgcagc tctgcctcgg
     5761 tgttagtatc gtctggttcg gcgtgccgct ccctgtcgcc gtgttgcaca acggcgtggc
     5821 cgccttgctc ctgttgaccg taatcaatgc taaccaacga attaggcaac gttagcagcg
     5881 ccggtgctca ctggcgcgac tactacgtgc tcacgaagcc gggcgtcgtg cagctgctcg
     5941 tcttcacggc catcgtcggc atgttcctcg cgacgccggg catggtgccg tgggagatcc
     6001 tgattgcggc cagcgtaggc atcggcctcg cagctgccgc cggcgccgtc gtcaatcaag
     6061 tgctcgatca acgcatcgac gcgcagatgg cccgcacgcg caatcgaccg ttgccgcagg
     6121 gccgcgtcac cgagcgcgac gcgctcgcgt tcgcgttggg cctggctttg ggcggcatcg
     6181 tggttctcgt gctgttcgtc aatccgctga cggccgctct gacgttcctg tcgatgatcg
     6241 gctatgccgt cgtctacacg gtctacttga agcgcgctac gccgcagaac atcgtcatcg
     6301 ggggcgccgc gggcgccgct ccgcccgtgc tcggttgggc ggccgtcacc aattccgtcg
     6361 acccgcacgc gctgctgttg ttcttgatca tcttcacttg gacgccgccg cacttctggg
     6421 cgctggcgat cgcgcgccgc acggactacg agcgagccga cattccgatg ttgccggtca
     6481 cgcacggcga agagctgacg aagtcgttcg tcatttacta cacgatcctg ctgctgctcg
     6541 tcacggtgct gccctcgatc acgggcatga cgggactctt ctacacgctc ggcgcgctgc
     6601 tgctcggcgg cggcttcctc tactacgcct tcatcctgaa gttcgcgcct cgccaagact
     6661 cgccgatggc gacgttccgt ttctcgatct ggtatctgat ggcgctgttc agcttcctgc
     6721 tgatcgacca ctacatcccg gccctctggc ccgcgtgatc gttcgatcgc cgacctagcc
     6781 cgcggcgctg ctcgcgaccc gatggacgtt accgacatca tcgacccttt gaacgaggcg
     6841 cagcgcgaag ccgtcgctgc ggccgatcgc ccgacgctcg tgctggcggg cgcgggcagc
     6901 ggcaagacgc gcgtgctcgt gcatcgcatc gcgtgggtca tccgcgtcaa cggcgtcgca
     6961 gcgcagcacg tcctggccgt cacgttcacg aacaaggcgg cggccgagat gcgcgaccgt
     7021 gtcgagtcgc tgctcggcgt gccgacacgc aacttgtgga tcggcacgtt ccacggcctc
     7081 tcgcatcgcc tcctgcgcat ccattggcgc gaagccggcc tcgccgaagg ctttcagatc
     7141 atggactccg aggaccagca gcgcatgatt cgccgcatgc tgcgcgcgct cgagctcgac
     7201 gagaacgtct gggtgccgaa ggagatccag tggttcatca acgcgcagaa ggacgagggc
     7261 cttaggccca aggacctgcg cgacgaaggc aacgcgggcc ggcgacagat gatcgacctc
     7321 tacgagcgct acgagcgcgt ctgcgcggct gcgtcggtca tcgatttcgg cgagctcctg
     7381 ctgcgcagcc tcgagctgct gcgcgacaac ctcgagctgc agcgccagta tcagcaccgc
     7441 ttccagcacg tgctcgtcga cgagttccag gacacgaacg ccatccagta tcgctggttg
     7501 cgcttgcttg cggggcccgc gggcacgccg ttcgtcgtcg gcgacgacga ccaatcgatc
     7561 taccgctggc gcggtgcgcg cgtcgagaat ctctatcgct ttcagcagga ctaccccggt
     7621 acgcagctcg tgcggctcga gcaaaactac cgctcgaccg ccaacatctt gaacgcggcc
     7681 aacgccgtca tcgcgaacaa cgcgtcgcgc ctcggtaaga cgctgtggac cgaaggcgag
     7741 gccggtgcgc cgatcaaggt ctacgccgcg ttcaacgatc gcgacgaggc cgagttcgtc
     7801 gtcaaccgca tccaagatca cgtcaatcag ggcggcaagc gcagcgaagc ggccgtgctg
     7861 tatcgctcga acgctcagtc gcgcgtgctg gaggaagggc tgctcgcggc cggcatgccg
     7921 tatcgcgtct acggcggctt gcgattcttc gagcgccagg agatcaagga cgcgctcgcg
     7981 tacttgcggc tcgtcatgaa tcgcgacgac gatccgtcgt tcgagcgcgt cgtcaacacg
     8041 ccgccgcgcg gcatcggcgc gcgcaccgtc gaagccgtcc gcgagcaagc gcgcgccgcc
     8101 ggcacgagtc tgtggcgtgc agcggcgacc gtgatcgccg ataaccggct cgcgaagcgc
     8161 gccaatgaat cgctgtgggc gttcctgagc ctcgtcgagt cgttggacaa ggatacgcgc
     8221 ggcgacgcgc tacacgacca ggtcgcgcgc gtcatcgagc gcagcggtct catggcgcat
     8281 tacggcaaga acccggatgc cggcggcgaa gcgcgtgtcg agaacctcga ggagctcgtc
     8341 aacgccgcgc gcgagatcat gcgctcggat gaaggcgagc tgccgccgct cgcgcagttc
     8401 ctcgcgttcg ccgtgctcga gtccggcgct ggccaggcgg aggcgtacca agacagcgtc
     8461 cagctcatga cgctgcactc cgcgaaaggc ctcgagttcg acctcttgtt cctctgcggc
     8521 atggaggacg ggctgtttcc gcatcagcgc tcgttgacgg atccggcggg gctcgaggaa
     8581 gagcggcgcc tctgctacgt cggcatcacg cgcgccaagc gcgagctgta cctgacgcac
     8641 gccgagcagc gccgcctgca cggcgaaatg cactattccg tgccgtcgcg cttcctgcgc
     8701 gaagtccccg ccgagctcat ggaggaggtc cgcccgcaga tggtcgtcgc gcgtccgatc
     8761 ttccgccgca gcgacgtaag cgagcctgtc ttcggcagcg cgctgaagct cggtcaacgc
     8821 gtgcggcacg gcaagttcgg cgaaggcatc gtgctgcgcc gctacggcag cggcgcgcac
     8881 acgctcgtcg aagtcaactt cgagtcgtcc ggcatgaaga ccctcgttct gacgcaggcc
     8941 aacctcgagc tgatgtagac tgct
//
LOCUS       DATLEE010000322         6161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4079929, whole genome shotgun sequence.
ACCESSION   DATLEE010000322 DATLEE010000000
VERSION     DATLEE010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4079929"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VM692_06915"
     CDS             complement(<1..1222)
                     /locus_tag="VM692_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HUQ51934.1"
                     /translation="MSELDDAAAREAALDPARSFIVQAPAGSGKTELLIQRYLALLAT
                     VQQPEQVVAITFTRKAAAEMRRRVLRALRAAADETPSEPAHERTAFALARAAIARDRA
                     LEWRLLEQPQRLRIDTLDAFNAWLARQLPVLADGVAAADVVDKADDLYREAARRCVVG
                     IAGDDGELGASLRTLLRDVGNDCEQLERLLAALLPRRDQWLRFLGGGDAELRLWLEGA
                     LQRLVDDEIAAAGALCEPRVLASLAPLLRHAAGAANDGLRAALEPWLALERAPGSGPV
                     ALAAWRGIAALLLTKQGDWRKRLAKPEGFGPEHTELRAKLTVLLDGLRDREPLRAALQ
                     TVASLPDARYTEAQWSSLAALRSVLVRLAAELKVAFAERRAVDFVELALAAQRALGQV
                     DSPSELLLALDRRIQ"
     gene            complement(1219..3762)
                     /locus_tag="VM692_06920"
     CDS             complement(1219..3762)
                     /locus_tag="VM692_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HUQ51935.1"
                     /translation="MAEDASAGSERSTIVVADALAAQQWEMHLAAAAIGGSAGAWETP
                     PVKSYAAWLDELWLEHADERGPALTVNQSFALWRRVVAESAESDELIGHAGAAEWAAG
                     AWGLLLRWQIDPATQRAAAQQDDYRALLGWCRAYRSRLDAHGWIDRHEIEAQLTHRGV
                     ARGRLVVADLPEPYPARTALLARLASSGLTIEERAAPAVPGTRRAAKLADAADELRAA
                     LTWARRHAASDPNARVAVVIPTAARRQDEIARLAAQGNGAAAQPHWSAGRALGTEPVI
                     GAAFDALALLDAHSAYATFGRWLRSPFFGLPPEEQYARARLDGELRAELRSQLPFQAA
                     YRAGLRELLAVRAPVAARALAAALDVIASVRRATPSRWAHLWSRCLVELGWQPPPRAA
                     LLGWQSTLDELAGLTPILGDVSFEHALTELARLLERTTPAALPLRGVHVLERIDAVGP
                     GYDAVWVTGFTDSAWPEAPRTNPLLPLALQRAHGMPYSSPADAHERSARALERLLQRS
                     RELVVSWPARVYDYDTEPSPAIRSWPTLATAELDALTAVRRPAAPPRESVDDEAGRFT
                     GARVPGGTGALGRQARCPVRAFCQDRLGARALEPLAFGVTARLRGIATHNAAERLLED
                     LPAQAALAAKSAAVPHSIERALTRVFGRTRHHLTALYDLEAEQLQRVLAALLHEEQLR
                     APFRVLAVEQRATIALGSLTLNVRVDRIDVLADGTLAIIDYKTGERATSAHWFGERLR
                     DAQVPLYASHAAVDVGAAVVARLVPTDTRYSGFWPTGAFPGRPSNAALQRDAQLELWR
                     AQLEVLAAEFAAGDTRIFVDDYDDAAADYAPLTRVFEQLAVARGAVARW"
     gene            complement(3762..5012)
                     /locus_tag="VM692_06925"
     CDS             complement(3762..5012)
                     /locus_tag="VM692_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUQ51936.1"
                     /translation="MSRHSHEIEASSQLKGVRYEIRGKLARRAHELERQGYEITQLNI
                     GNPGVFGLRTPETMRLAMIENLPSAEAYCNQKGIFPAREAVVMQQQARGVDGVDADHV
                     FMGNGVSELIDLTLRGLLNPGDEVLIPSPDYPLWTAATTLNGGRAVYYPCPESQGFNP
                     DPEAVEKLVTPRTRAIVIINPNNPTGAVYSRERLTALARIAERHNLVVMSDEIYDQML
                     YDGAEFVPMATLCHDTLVATFSGLSKIYRACGYRVGWVSFSGAVDRSDKYMSALDLLA
                     ALRLCSNVPGQWAVQTALGGFQSIHKLCSPGGRLYQSRKAILDGVARSRFMSVVAPRG
                     AMYAFIGIDKQKLPGFDDEAFAMELLEQQHVLLAPGSSFNTPYRDHFRITNLPEDTRL
                     SEVFQRIEHVLEQIADRPPRLETA"
     gene            complement(5116..5970)
                     /locus_tag="VM692_06930"
     CDS             complement(5116..5970)
                     /locus_tag="VM692_06930"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HUQ51937.1"
                     /translation="MTFGPLVDVDTLARHLGRPDWVVVDCRFTLTDPPAGRAAYDRGH
                     IPGARYAHLDDDLARRPSAHEGRHPLPEPQRFAATLGAWGIDRATTVVAYDEASGAVA
                     ARLWWMLGWLGHERCAVLDGGFAAWQGAGQPLEQNAPSIAARRYEPRQLAHDAVVATR
                     DLAARIAAGDLLVDARAAPRYRGEQEPIDPKAGHVPGARNRPFSTNVTATGGFRPPAE
                     LRTELAELLDGRGAGRVVAMCGSGVTACHLLLALEIAGLGRGRLYAGSWSEWIRDPAR
                     PIRTGAEP"
     gene            6064..>6161
                     /locus_tag="VM692_06935"
     CDS             6064..>6161
                     /locus_tag="VM692_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EtfB protein"
                     /protein_id="HUQ51938.1"
                     /translation="MVGIKRVVDYNVRVRVKPDGSGMATAGLKMSI"
BASE COUNT          924 a         2139 c         2186 g          912 t
ORIGIN      
        1 gttgaatgcg gcgatcgagc gcgagcagca gctccgacgg cgagtcgacc tggccgagcg
       61 cgcgctgggc cgcgagcgcg agctcgacga agtcgacggc gcgccgctcg gcgaacgcga
      121 ctttgagctc ggcggcgagg cgcacgagca cgctgcgtag cgcagcgaga ctcgaccact
      181 gcgcttccgt gtagcgcgcg tccggcaacg aggcgacggt ctgcagcgcg gcgcgcagcg
      241 gctcacgatc gcgcagcccg tcgagcagca ctgtgagctt tgcgcgcagc tccgtgtgct
      301 ccgggccgaa gccctcgggc ttcgccaagc gcttgcgcca atcgccttgc ttcgtcagca
      361 gcaacgctgc aatgccgcgc caggccgcca acgcgacggg gcccgagccc ggcgcgcgct
      421 cgagcgcgag ccaaggctcg agcgcggcac ggaggccgtc gttcgcggct ccggccgcgt
      481 gccgcaacaa cggcgccaac gacgcaagca cgcgtggctc gcataacgcg cccgcggccg
      541 cgatctcatc gtcgacgagc cgctgcagcg ctccctcgag ccagagacgc agctcggcat
      601 cgccgccgcc caggaagcgc agccattgat cgcgccgcgg cagcaacgcc gcgagcagcc
      661 gctcgagctg ctcgcaatcg ttgccgacgt cgcgtagcaa cgttcggagg ctggcgccga
      721 gctcgccgtc gtcgcccgcg attcccacga cgcagcggcg cgcggcctcg cggtacaggt
      781 cgtcggcctt gtcgacgacg tcggccgccg caacgccgtc ggccaggacc ggcaattggc
      841 gcgcaagcca cgcgttgaac gcgtcgagcg tatcgatgcg caagcgctgc ggttgctcca
      901 gcagccgcca ttcgagggct cggtcgcgcg cgatggcggc gcgcgcaagc gcgaaggcgg
      961 tacgctcgtg cgccggctcg ctcggcgtct cgtccgcggc ggcacggagt gcgcgcagca
     1021 ctcggcggcg catctcggcg gccgccttgc gcgtgaacgt aatggcgacc acctgctccg
     1081 gttgctggac tgtcgcgagc agtgccagat agcgctgtat caagagctcc gtcttgccgg
     1141 agccggccgg agcttgaacg atgaacgacc gcgcaggatc gagcgcggct tcgcgcgcgg
     1201 cggcgtcgtc gagctcgctc accaacgtgc caccgcgccg cgggccactg cgagctgctc
     1261 gaagacgcgc gtcaacggcg catagtcggc ggccgcatcg tcgtagtcgt cgacgaagat
     1321 gcgagtgtcg ccggccgcga actccgcagc gagcacctcg agctgcgcgc gccacagctc
     1381 gagctgcgca tcgcgctgca acgctgcatt gctcggacgg cccgggaaag cgccggtcgg
     1441 ccagaacccc gagtatcgag tgtcggtcgg gaccagccgc gcgacgacgg cggcgccgac
     1501 gtcgaccgct gcatggcttg catagagcgg cacctgcgca tcgcgcagcc gctcgccgaa
     1561 ccagtgcgcg ctcgtcgctc gctcgccggt cttgtagtcg atgatcgcca gtgtgccgtc
     1621 ggcgagcacg tcgattcggt cgacacgcac gttcagcgtc aacgatccaa gcgcgatcgt
     1681 cgcgcgctgc tcgacggcga gcacgcggaa cggcgcgcgc agctgttcct cgtgcagcaa
     1741 cgcagcgagc acgcgctgca actgctcggc ctcgaggtcg tacagcgccg tcagatggtg
     1801 ccgcgtgcgg ccgaacacac gcgtcagggc gcgctcgatg ctgtgtggga cggcggctga
     1861 ctttgctgcc aatgccgctt gcgcgggcaa gtcctcgagc aggcgctccg ccgcgttgtg
     1921 cgtagcgatg ccgcggaggc gcgctgtgac gccgaaggcg agcggctcga gcgcgcgggc
     1981 gccgagccgg tcttggcaaa acgctcgcac ggggcaacgc gcttggcggc ccagtgcgcc
     2041 cgtgccgccc gggacgcgcg cgccggtgaa tcgccccgct tcgtcgtcga cgctttcacg
     2101 cggcggggcc gcggggcgcc ggacggctgt caacgcgtcg agctcggcgg tcgccagcgt
     2161 cggccaggag cgaattgcag ggctcggctc ggtgtcgtag tcgtagacgc gcgccggcca
     2221 gctcacgacg agctcgcggc tgcgttgcag caggcgctcg agtgcgcgcg ccgagcgctc
     2281 gtgcgcgtcg gccggcgacg agtacggcat gccgtgcgcg cgttgcagcg cgagcggcag
     2341 cagcggattg gtgcgcggcg cctccggcca agccgagtcg gtgaagcccg tgacccagac
     2401 ggcgtcgtag ccggggccga cggcgtcgat gcgctcgagc acgtgcacgc cgcgcagcgg
     2461 caacgcggcc ggcgtcgtgc gctcgagcag tcgagcgagc tccgtcaacg cgtgctcgaa
     2521 cgagacgtcg ccgagaatcg gcgtcagccc tgcgagctcg tcgagcgtgc tctgccagcc
     2581 gagcaaggca gcgcggggcg gcggttgcca accgagctcg acgaggcagc gcgaccacag
     2641 atgcgcccaa cggctcggcg tcgcgcggcg cacgcttgcg atgacgtcga gcgccgcggc
     2701 gagagcgcgc gcagccacgg gcgcgcgcac tgcgagcagt tctcgtaggc cagcccgata
     2761 ggccgcttgg aacggcaact gcgaacgcag ctcggcgcgc agctcgccgt cgaggcgtgc
     2821 gcgcgcatat tgctcctcgg gcggcaggcc gaagaacggg ctgcgcagcc agcggccgaa
     2881 cgtcgcatac gcagagtgcg cgtcgagcag cgccagcgcg tcgaacgcgg caccgatgac
     2941 cggctccgtg ccgagcgcgc ggccggcgct ccagtggggc tgcgctgctg cgccgttgcc
     3001 ctgcgcggcg aggcgcgcga tctcgtcttg ccgtcgcgcc gcggtcggga tcacgacggc
     3061 gacgcgcgcg ttcgggtcgc tcgcggcatg tcggcgcgcc catgtcagcg ccgcgcgcag
     3121 ctcgtcggcc gcgtcggcca gcttcgccgc acggcgtgtg cccggcacgg ccggcgccgc
     3181 gcgctcctcg atcgtgaggc cgctcgaagc gaggcgtgcc agcaatgcgg ttcgggccgg
     3241 atacggctcg ggcagatccg cgacgacgag ccggccgcgc gctacgcctc ggtgcgtcaa
     3301 ctgcgcttcg atctcgtgcc ggtcgatcca gccgtgagcg tcgagccggc tccgataggc
     3361 gcggcaccaa ccgagcaatg cgcggtagtc gtcttgctgc gccgcggctc gctgcgtggc
     3421 cggatcgatt tgccagcgaa gcaataggcc ccacgcgccg gccgcccatt cggctgcgcc
     3481 ggcatgaccg atcagctcat cgctctccgc ggactctgcg acgacgcgcc gccagagcgc
     3541 gaacgattga ttgaccgtga gcgcgggacc gcgctcgtcg gcgtgctcga gccacagctc
     3601 gtcgagccac gccgcgtacg acttcacggg cggcgtttcc catgcaccgg cactgccgcc
     3661 gatcgccgcg gccgccaaat gcatctccca ctgctgcgca gcgagcgcgt cggctacgac
     3721 gatggtcgag cgctcgctgc cggcgctcgc gtcttccgcc atcaggccgt ttcgagccgc
     3781 ggcggtcgat cggcgatttg ctcgaggacg tgctcgatgc gttggaagac ttcggacagc
     3841 cgcgtgtcct cgggcagatt cgtgatgcgg aagtggtcgc gatacggcgt gttgaagctc
     3901 gagcccggcg cgagcaagac gtgctgctgc tcgagcagct ccatcgcgaa cgcctcgtca
     3961 tcgaagcccg gcaacttctg cttgtcgatg ccgatgaacg cgtacatcgc gccgcgcggt
     4021 gccacgacgc tcatgaaacg gctgcgcgct acgccgtcga ggatggcctt gcgcgattga
     4081 tacaacctac cgcccggcga gcacagcttg tggatgctct ggaacccgcc gagcgccgtc
     4141 tgcacggccc attgacccgg cacgttgctg caaaggcgca gcgcggccaa gagatcgagc
     4201 gcgctcatgt atttatcgga gcgatcgaca gcgccgctga acgagaccca gccgacgcga
     4261 tagccgcacg cgcgatagat cttcgacagg ccgctgaacg tcgcgaccaa cgtgtcgtgg
     4321 cacaacgtcg ccatcggcac gaactccgcg ccgtcgtaga gcatctggtc gtagatctcg
     4381 tcgctcatga cgacgaggtt gtggcgctcg gcgatgcgtg cgagggccgt caatcgctcg
     4441 cggctgtaga cggccccggt tggattgttc gggttgatga tgacgatcgc gcgcgtgcgc
     4501 ggcgtgacga gtttctcgac ggcctcgggg tccggattga agccctgcga ctcggggcac
     4561 gggtaataga ccgcgcgccc gccgttcaac gtcgtagccg ccgtccacaa cgggtagtcg
     4621 gggctcggga tcaagacctc gtcgccgggg ttcaacaggc cgcgcagcgt gagatcgatg
     4681 agctcgctga cgccgttgcc catgaacacg tgatcggcgt cgacgccgtc gacaccgcgg
     4741 gcttgctgct gcatgacgac ggcttcacgc gccgggaaga tgcctttctg attgcagtag
     4801 gcctcggccg acgggaggtt ctcgatcatc gcgagccgca tcgtctccgg agttcgcaga
     4861 ccgaacacgc cggggttgcc gatgttcagc tgcgtgattt cgtagccttg ccgctcgagc
     4921 tcgtgcgcgc gtcgggcgag cttgccgcgg atctcatagc ggacgccctt gagctgtgag
     4981 ctcgcttcga tctcgtgaga atgacgcgac acgacgattc ctttcgaatg ctcgatgaat
     5041 aggtcggctg tctttaccac gccgcgcggc tcgggccaag cgcttgcgtc gcagtgggcg
     5101 ccgtccgcaa gccgtttacg gctcggctcc ggtccttatc ggccgggccg gatcgcgtat
     5161 ccactcgctc caagacccgg cgtagagtcg gccgcgcccg agcccggcga tttcgagggc
     5221 cagcagcagg tggcacgccg tgacgcccga gccgcacatc gcgacgactc tgcccgcccc
     5281 gcgtccgtcc aagagctcgg ccagctcggt gcgcagctcc gccggtggcc ggaacccccc
     5341 ggtcgccgtg acgttcgtgg aaaacggccg attgcgagcg cccggcacgt gccctgcctt
     5401 gggatcgatc ggctcctgct cgccgcgata gcgcggcgcc gctcgcgcgt cgacgagcaa
     5461 gtcgcccgct gcaatgcgcg cggcgaggtc gcgcgtcgcg acgacggcat cgtgcgcgag
     5521 ctgccttggc tcgtagcggc gagccgcgat gctcggtgcg ttttgctcca gcggctggcc
     5581 cgcgccctgc caggcggcaa agccgccgtc gagaacggcg cagcgttcgt gcccaagcca
     5641 gcccagcatc caccacagac gcgcggcgac ggcgccgctt gcctcgtcgt aggcgacgac
     5701 cgtagttgct cgatcgatgc cccacgcgcc gagagttgcc gcgaagcgtt gcggctccgg
     5761 taatggatgg cggccttcgt gcgcactcgg gcgacgcgcg aggtcgtcat cgagatgcgc
     5821 atagcgagcg ccgggtatgt ggccgcgatc gtaggccgcg cgcccggcgg gcgggtccgt
     5881 caacgtaaaa cggcaatcca cgacgaccca gtccggccgg ccgagatggc gcgccaaggt
     5941 gtcgacgtcg acgagcggac cgaatgtcat cgcgccattg tagcgatcgc tgggccgagt
     6001 cgccgcgatg ctaaaatttg cggtgcgaag ttcgcacagg gagttgtcga tttgcagtcc
     6061 gtgatggtcg gtatcaagcg cgttgtcgac tacaacgtgc gcgtgcgcgt caagcccgac
     6121 ggctcgggca tggcgaccgc gggcttgaaa atgagcatca a
//
LOCUS       DATLEE010000323         2648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7636268, whole genome shotgun sequence.
ACCESSION   DATLEE010000323 DATLEE010000000
VERSION     DATLEE010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7636268"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VM692_06940"
     CDS             complement(<1..1196)
                     /locus_tag="VM692_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUQ51939.1"
                     /translation="MSTVFKHQYDTSHLQGANATYVEWLYEQYLAEPGSVPPQWRQYF
                     GSLAPTSAEIPHGPIVQAVAQRLRQRGAANGAAAPAESAPAIEKQAAVSRLIQVYSLR
                     GHQIADLDPLGLWERPAPAVLSFDFLGLDESDLAREFSTGGLAGTGHARMKLQDILAL
                     LKQIYCGKLAAEFAHISRARERLWLREQFEASRVQCEFAAAEQTTILDSLTRAEGIER
                     YLNTRFVGQKRFSLEGSETLIPVLDDLIQRAGEVGVQELVIGMAHRGRLNVLVNVLGK
                     SPQDLFSEFEGAYDLARLKGSGDVKYHKGYSADVRTPGGNVHIALAFNPSHLEIVNPV
                     VEGSVRARQDRRGDTLGEQVLPVLIHGDAAFAGQGVVAETFQLSQTRAFYTGGTVHLV
                     INNQIG"
     gene            complement(1299..1793)
                     /locus_tag="VM692_06945"
     CDS             complement(1299..1793)
                     /locus_tag="VM692_06945"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HUQ51940.1"
                     /translation="MTDSFDRSAASAELALALPVERRRGPDRRRLTLRSFLKGGITPR
                     RRGGRRADEQHLPIDWHGPYLLFLATTILLLSVADAFLTITLITLGAQEANPVLAFIL
                     QEHPELFAIMKMGLTGSGVLVLVAVARARLFRVMRVGVILQGVFVAYVALIAYEWWLL
                     RSFL"
     gene            complement(1933..2433)
                     /locus_tag="VM692_06950"
     CDS             complement(1933..2433)
                     /locus_tag="VM692_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUQ51941.1"
                     /translation="MSARASQRRLVPVLGLLLAACDGGTAVAPSTAPPSAAVTVRAAG
                     FDPGPVKSAAEYLLEPEYAGADFAHGELLGLACAACHRFGAGEGTLIGPNLHGVFGRA
                     AATVEGFDYSPALRQSGLVWTPLALSAWLADPAGFVAGTTMPFTGYKAEQDRRDLIAY
                     LLRATE"
     gene            complement(2430..>2648)
                     /locus_tag="VM692_06955"
     CDS             complement(2430..>2648)
                     /locus_tag="VM692_06955"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HUQ51942.1"
                     /translation="VLPKFGLKGQLDIHGAFYESPLEEIGEPAIQATLSAWRRTGQRG
                     TIEFAVVEDLNVSTSPDIVLQVAAAWQW"
BASE COUNT          501 a          869 c          860 g          418 t
ORIGIN      
        1 aagccgatct gattgttgat gacgagatga accgtgccgc ccgtgtagaa cgcacgcgtc
       61 tgcgacagtt gaaacgtttc cgcgacgacg ccctggccgg cgaacgccgc atcgccgtga
      121 atcagcacgg gcagcacttg ctcgccgagc gtatcgccgc gacggtcctg gcgcgcgcgc
      181 acggagcctt cgacgacggg attgacgatc tcgaggtgcg acggattgaa cgcgagcgca
      241 atgtgcacgt tgccgcccgg cgttcgcacg tcggccgagt agcccttgtg gtatttgacg
      301 tcgcccgagc ccttgagccg cgcgaggtcg tacgcgcctt cgaattccga gaacaaatcc
      361 tgcggcgact tgccgagaac gttgacgagc acgttcaacc ggccgcgatg cgccatgccg
      421 atgacgagct cctgcacgcc cacttcgccg gcacgttgga tcagatcgtc gagtaccggg
      481 atcagcgtct cgctaccttc gagcgagaag cgcttttggc cgacgaagcg cgtgttcaga
      541 tagcgctcga tgccctcggc gcgcgtcaaa ctgtcgagga tcgtggtttg ttccgcggcc
      601 gcgaactcgc actgcacgcg gctggcctcg aactgctcgc ggagccagag ccgctcgcgg
      661 gcgcgcgaga tgtgcgcgaa ctcggcggcg agcttgccgc agtagatttg cttcagcagc
      721 gccaggatgt cctggagctt catgcgcgcg tggcccgtgc cggccaaccc gcccgtcgaa
      781 aactcgcgcg cgaggtccga ttcgtccaag ccgaggaaat cgaagctcaa cacggcgggc
      841 gcgggccgct cccagaggcc gagcggatcg agatccgcga tctggtggcc gcgaaggcta
      901 taaacctgaa tcagccgcga gacggcggct tgcttctcga tcgcaggcgc cgattcggca
      961 ggcgccgctg caccgtttgc agcaccgcgc tgccgcagcc gctgcgctac ggcctgaacg
     1021 atcggcccgt gaggaatttc ggcgctcgtc ggcgccagcg atccgaagta ttgacgccac
     1081 tgcggcggga cgctgcccgg ctcggcgagg tactgctcat agagccattc gacgtaggtc
     1141 gcgttggctc cctgcaggtg cgacgtgtcg tactggtgct tgaatactgt gctcatgagc
     1201 ttgggcgtga cggcgtaggt gcagacgatt atgcggccgc caggccctaa ggcgcgcgta
     1261 gtttagccaa tcgagcatca agttcaaacg cgcttacgtc acaggaatga gcgcagcaac
     1321 caccattcgt acgcgatcaa tgcgacgtac gccacaaaaa caccttgcag gatgaccccg
     1381 acgcgcatga ctcgaaacaa acgcgcgcgc gcgaccgcga cgagcacgag cacaccgctg
     1441 cccgtgaggc ccatcttcat gatcgcgaac aactccggat gctcctgcag gatgaatgcc
     1501 aagaccggat ttgcttcttg cgcgccgagc gtgatcagcg tgatcgtgag gaaggcgtcg
     1561 gcgacactga gcagcaaaat tgtcgtcgcc agaaacaata gatacggtcc gtgccagtcg
     1621 atcggcagat gctgctcgtc ggcgcgacgt ccgccgcgac ggcgcggcgt gatgccaccc
     1681 ttcaagaaac tgcgcagcgt gagccgacgg cgatccggtc cgcggcgtcg ctcgacgggg
     1741 agagcgaggg caagctcggc gctcgccgcg cttcgatcaa aggagtcagt caagcttgaa
     1801 accccagtaa acgaatgagt accgggatca atgcaagaac caagccgcag ctggcgcgaa
     1861 gccaatcccg tcagcctttt cagctgcttg gacgcgcaat agagccccct atgtaaaact
     1921 tcccgacgcg gcctattccg ttgcccgcag caaataagca atgagatcgc ggcgatcttg
     1981 ctcggctttg tagcctgtaa acggcatcgt cgtgccggcc acgaacccgg cgggatctgc
     2041 gagccacgcg gatagcgcca gcggcgtcca aaccaggccg gattgccgga gggccgggga
     2101 gtaatcgaac ccctcgacgg tcgccgccgc gcggccgaac acgccgtgca agttggggcc
     2161 gatcaaggtg ccctcgcccg cgccgaagcg gtggcacgcg gcgcaggcga gaccgagcaa
     2221 ctcgccgtgt gcgaaatcgg cgcccgcgta ctcgggctcg agcaagtact cggccgcgga
     2281 cttgacggga ccgggatcga acccggccgc gcgcaccgtg acggccgccg aaggcggcgc
     2341 cgtcgagggc gcgaccgccg tgccgccgtc gcaagcggcg agcaacaatc cgagcacagg
     2401 cacgagtcgc cgctgcgacg ctcgcgcgct caccattgcc acgcggccgc gacctgcagc
     2461 acgatgtcgg gcgacgtgct gacgttcaag tcctcgacga cggcgaactc gatcgtgccg
     2521 cgctgcccgg tccggcgcca cgcgctcagc gtcgcctgga tcgccggctc gccgatctct
     2581 tcgagcggtg actcgtagaa cgcgccgtgg atgtcgagct gccccttgag accgaacttc
     2641 ggcaagac
//
LOCUS       DATLEE010000324         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8383069, whole genome shotgun sequence.
ACCESSION   DATLEE010000324 DATLEE010000000
VERSION     DATLEE010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8383069"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VM692_06960"
     CDS             <1..998
                     /locus_tag="VM692_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ51943.1"
                     /translation="HFQTVHHDLFDYDLPAAPILIDINVGGRPIKALAQVTKHGFLFV
                     LDRATGAPVWPIEERPAPKSTVPGEMASPTQPHPTKPKPYEQQGLTENDLIDFTPQLR
                     AEALEIVKRYVIGPAFTPPSVIDTAPNGKLGTIQLPGASGGANWNGGAFDPETGMFYV
                     GSRSVAFVADLFAPDPARSDLRYRAGMREIMNGPRGLPIMKPPDGRITAFNLNTGDTA
                     WTVPNADGPRNHPALAGLNLPPLGDATPSAVIATKTLLFATQADKSTPRSPANVGGTK
                     FMALDKATGKTLWSIDLGVGQNGTPMTFQHDGKQYVVVAVGSTDYPAELVALALP"
     gene            1019..>2004
                     /locus_tag="VM692_06965"
     CDS             1019..>2004
                     /locus_tag="VM692_06965"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51944.1"
                     /translation="MRRVVLGCAISLACAVPAAAHHGPGTFDLRSNVAYPVATLTGVD
                     MINPHSWLYFEVKQPDGSVSKHRCEMRSVHTLRRSGWTEDLFPKGVQIAIEASPDRAD
                     PNSCYLQTITFANGSHMDRYGQYVKGVDGGVTEVRGPVVAVNTAQRAFRGANGKPNLA
                     GDWAQEQRVMANPRGTGGGLVPLGQLTEIDRDRSTAAGGAAPARPAGGAGGPGAAPAG
                     PRLYGGTALTPAGEAAAAAFKREDNPRFSCETTSILFDWTFDGAVNRIEQTDSTITLR
                     YGQMGLVRTIHMNGAAGAANAAPSRAGYSVGRWDGDVLVVETTRFAPGVLNAP"
BASE COUNT          339 a          685 c          671 g          309 t
ORIGIN      
        1 ggcacttcca gacggtgcac cacgatttgt tcgattacga tttgcctgcc gcgccgatcc
       61 tgatcgacat caacgtcggt gggcggccga tcaaagcgct cgcgcaggtc acgaagcacg
      121 gcttcctgtt cgtgctcgat cgcgcgacgg gcgcacctgt gtggccgatc gaagagcgcc
      181 ccgcgccgaa gtcgacggtg cccggcgaga tggcgagccc gacgcagccg catccgacta
      241 aacctaagcc gtacgagcag cagggcctga ccgagaacga cctcatcgat ttcacgccgc
      301 agctgcgcgc cgaagcgctc gagatcgtca agcgctacgt catagggccg gcgtttacgc
      361 cgccgtcggt catcgatacg gcgccgaacg gcaagctcgg cacgatccag cttccgggcg
      421 cgtcgggcgg ggcgaattgg aacggcggcg cgttcgatcc cgagaccggc atgttctacg
      481 tcggctcgcg cagcgtcgcg ttcgtcgcgg acttgttcgc gcccgatccc gcgcgctcgg
      541 acctgcgcta tcgcgccggc atgcgcgaga tcatgaacgg cccgcgcggg ctgccgatca
      601 tgaagccgcc ggacggccgg atcacggcgt tcaatctgaa cacgggcgac accgcgtgga
      661 cggtgccgaa cgccgacggt ccgcggaatc atccggcgct cgccggcttg aatctgccgc
      721 cgctcggcga cgcaacgccg tcggccgtca tcgcgacgaa gacgctgttg ttcgccacgc
      781 aggccgacaa aagcacgccg cgctcgccgg cgaacgtcgg cggtacgaag ttcatggcgc
      841 tcgacaaagc gaccggcaag acgttgtggt cgatcgatct cggcgtcggc cagaacggca
      901 cgccgatgac gttccagcac gacggcaagc aatacgtcgt cgtggcggtc ggcagcacgg
      961 actatccggc cgagctcgtc gcgctcgcct taccttgatg tacacgatgg aggcttccat
     1021 gcgtcgcgtc gtactcggtt gcgccatctc gcttgcctgc gccgtacccg ccgccgctca
     1081 ccacggcccc ggcacgttcg acttgcgcag caacgtcgct tatccggtcg cgacattgac
     1141 gggcgtcgac atgatcaatc cgcactcgtg gctctacttc gaagtgaagc agcccgacgg
     1201 cagcgtctcg aagcaccgct gcgagatgcg ctccgtgcac acgctgcgcc gctcgggttg
     1261 gaccgaagac ttgtttccca agggcgtgca gatcgcgatc gaagcatcgc cggaccgcgc
     1321 cgatccgaat tcctgctact tgcagacgat cacgttcgcg aacggcagcc acatggaccg
     1381 ctacgggcag tacgtgaaag gcgtcgatgg cggcgtcacc gaggtgcgag gtcccgtcgt
     1441 cgcggtgaac acagcgcagc gtgcgttccg cggcgcgaac ggcaagccga atctcgccgg
     1501 cgactgggcg caggagcagc gcgtgatggc gaacccgcgc ggcacgggcg gcggcttggt
     1561 cccgctcggt cagctcacgg agatcgatcg agacaggagc acagccgccg gtggcgccgc
     1621 tccggcgcgc ccggccggtg gcgccggtgg tccgggcgcg gcaccggccg gcccgcggct
     1681 ctatggcggc accgcgctga cgcctgccgg cgaagccgcg gctgccgcgt tcaagcgcga
     1741 agacaatccg cgcttcagct gtgagaccac gagcatcctc ttcgattgga cgttcgacgg
     1801 cgccgtcaac cgcatcgagc agaccgacag cacgatcacg ttgcgttacg gccagatggg
     1861 tctcgttcgt acgatccaca tgaatggagc tgcgggcgcg gcgaacgctg cgccgagccg
     1921 cgcaggctat tccgtcgggc gctgggacgg cgacgtgctc gtcgtcgaga cgacgcgatt
     1981 cgcgcctgga gtgctgaacg cgcc
//
LOCUS       DATLEE010000325         2657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6895716, whole genome shotgun sequence.
ACCESSION   DATLEE010000325 DATLEE010000000
VERSION     DATLEE010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6895716"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VM692_06970"
     CDS             complement(<1..725)
                     /locus_tag="VM692_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HUQ51945.1"
                     /translation="MTDSSAVSGPGLHRVIGPKLLLFLVIGDMLGTGIYALTGDVAGR
                     VGGAAWLAFGGAFIVALFTATSYLELVGKFPSAAGAALYSQRAFNSRFVTFLVGFAVM
                     CSALTSVAAASRAFSGYFQELAAVPLVVLVPLFILGLAYINFRGVGESVKINVAMTCV
                     ELAGLLIVIFVGAWALSQGIGEPANALEFNSDGEPFGLVVSGAALAFFAIIGFEDSVN
                     MVEETTNPQRDFPRAFFIGITLTG"
     gene            847..1893
                     /locus_tag="VM692_06975"
     CDS             847..1893
                     /locus_tag="VM692_06975"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51946.1"
                     /translation="MNARLAALLVPLLAAPAAYAHHSFAPHFDIDKPVDISGKVVAYE
                     ARNPHSYLHIAALDENGKTQEYVCESHGVTQLTRNGITPEMLKPGTELRVDGSLSRHS
                     AYMCFFNNVYFPDGRALSVNGNRATQAPRALPPRTDIFGTWLLAPAAPDPRRANTSRP
                     QPMINFLTAAGQAAVAAYDPFKDDPTFRCDPVAIRRVWGAPGTPLSVTREGDTVVLRH
                     EWMDVERTVHLNMREHPANGPRTSLGHSIGHFEGDTLVIETANYSAGVLNQYVEEPGK
                     PTRGLLHSAALSSVERLSFDAERQRLVVEVALKDPEFFTRDFDVSRSEYAPSDLSIEP
                     FNCSPEGVTGTIKQ"
     gene            complement(2073..>2657)
                     /locus_tag="VM692_06980"
     CDS             complement(2073..>2657)
                     /locus_tag="VM692_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HUQ51947.1"
                     /translation="QKNRIKRMIISTYQAASGAGAAAMEELRESTRAYLDGKPFQHKV
                     LPHPYAFNAFSHNTKIDPATAHNEEETKVINETRKIFGDPNIAIGITCVRIPVLRAHC
                     VSVTFECEKPITPEEVRAIISKAPGCRVVDDVERNYFPMPVDASGKDEILVGRIRRDL
                     SDSTGRSISIFSAGDQLLKGAALNAVQIAEELLA"
BASE COUNT          475 a          876 c          859 g          447 t
ORIGIN      
        1 ccggtcaacg tgatgccgat gaagaacgcg cgcgggaaat cgcgctgcgg attcgtcgtc
       61 tcctcgacca tgttcacgga gtcttcgaag ccgatgatcg cgaagaacgc gagcgcggcg
      121 ccgctgacga cgagcccgaa cggctcgccg tccgagttga actcgagcgc gttggcaggc
      181 tcgccgatgc cctgcgagag tgcccacgcg ccgacgaaga tgacgatcaa caggcccgca
      241 agctcgacgc acgtcatcgc gacgttgatc ttcacggact cgccgacgcc gcggaagttg
      301 atgtaggcga gccccaggat gaacagcggc acgagcacga cgagcggcac ggccgccagc
      361 tcctggaaat accccgagaa cgcgcggctc gcggcggcca cggacgtcaa cgccgagcac
      421 atgacggcga agccgacgag gaacgtcacg aagcggctgt tgaacgcgcg ttgcgagtaa
      481 agcgccgcgc cggccgcgct tgggaatttg ccgacgagct cgaggtacga ggtcgccgtg
      541 aacagtgcga cgatgaacgc cccgccgaac gcgagccacg cggcgccgcc gacgcgtccc
      601 gcgacgtcgc ccgtcagcgc atagatgccg gtgccgagca tgtcgccgat cacgaggaac
      661 agcagcagct tggggccgat gacgcggtgc aggccggggc cgcttaccgc agacgagtcg
      721 gtcacggtgg tgccgcttcg gggactatca aggggagtcg tcattgcgcg gcacgctagc
      781 aaaaaccgct gcgctaagcg acgggcgcgg aatatactcg ttcgtcgtca ggggggaaga
      841 cacgcgatga acgcacgact cgccgcattg ctggtcccgc tgctcgcagc gcccgccgcc
      901 tacgcgcacc acagcttcgc gccgcacttc gacatcgaca agcccgtcga catctcgggg
      961 aaagtcgtcg cgtatgaagc gcgcaatccg cacagctatc tgcacatcgc cgcgctcgac
     1021 gagaacggca agacgcagga atacgtctgc gagtcgcacg gcgtcacgca gctcacgcgc
     1081 aacggcatca cgccggagat gttgaagccg ggcacggagc tgcgcgtgga cggctcgctg
     1141 tcgcggcaca gcgcctacat gtgtttcttc aacaatgtct acttcccgga cggccgcgca
     1201 ttgagcgtga acggcaacag ggcgacgcaa gcgccgcgcg cattgccgcc gcgcaccgac
     1261 attttcggca cctggttgct ggcgcccgcc gcgccggacc cgcggcgcgc caacacgagc
     1321 cgcccgcaac cgatgatcaa tttcttgacg gcggccggcc aagctgcggt cgcagcctac
     1381 gacccgttca aggacgaccc gacgttccgc tgtgatcccg tcgcgatccg ccgcgtgtgg
     1441 ggcgcgcccg gcacgccgct atcagtcacg cgcgagggcg acaccgtcgt tctgcggcac
     1501 gaatggatgg acgtcgagcg caccgtacac ctgaacatgc gcgagcatcc ggccaacggt
     1561 ccccgcacgt cgctcggcca ttcgatcggc catttcgaag gcgacacgct cgtcatcgag
     1621 acggcgaact actcggccgg cgtgctgaac cagtacgtcg aggagccggg caagccgacg
     1681 cgcggactgc tgcactcggc ggcgctgagc tcggtggaac gcttgtcgtt cgatgcggag
     1741 cgccagcggc tcgtcgtcga agtggcgctc aaggatccgg agttttttac gcgcgacttc
     1801 gacgtgtcgc gctccgagta cgcgccgtcg gacctttcga tcgagccgtt caactgctcg
     1861 cccgagggcg tcacgggcac gatcaaacag taaccgacgg cgctcgagag cttacgcttt
     1921 gcgcgcctct ttcttggcgc gcttcatcgc cttcttttct ttcggcgtca acagcggctt
     1981 cttcttgcct tctttctgtg tcgtgcgttc tttggccatg ggcgatcgct ccttgtcgat
     2041 tacgaagcga caatgtaagc acgcttaaac gattacgcca acagctcctc ggcgatctgc
     2101 acggcgttca acgcggcgcc cttcagcagc tgatcgccgg ccgagaaaat cgagatcgag
     2161 cggcccgtcg aatcgctcaa gtcgcgacgg atgcggccga cgaggatctc gtccttgccc
     2221 gacgcgtcga cgggcatcgg gaagtaattg cgctcgacgt cgtcgacgac gcggcagccc
     2281 ggcgccttcg agatgatcgc gcggacttcc tcgggcgtga tcggcttctc acactcgaac
     2341 gtcacggaga cgcagtgcgc gcgcagcacg ggaatgcgca cgcaggtgat gccgatcgcg
     2401 atgttcggat cgccgaagat cttgcgcgtc tcgttgatga ccttcgtctc ttcctcgttg
     2461 tgcgccgtgg ccgggtcgat cttcgtgttg tggctgaacg cgttgaacgc gtacgggtgc
     2521 ggcagcacct tgtgctgaaa cggcttgccg tccaagtacg cgcgcgtcga ttcgcgcagc
     2581 tcttccatgg cggcggcgcc ggcgcccgac gcggcttggt acgtcgagat gatcatccgc
     2641 ttgatgcggt tcttttg
//
LOCUS       DATLEE010000326         5042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8383338, whole genome shotgun sequence.
ACCESSION   DATLEE010000326 DATLEE010000000
VERSION     DATLEE010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8383338"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VM692_06985"
     CDS             <1..148
                     /locus_tag="VM692_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HUQ51948.1"
                     /translation="PRVMNGFSDLILELFGPDRGAHSRSAVGMAELPFDLPVEIEAEL
                     EIEP"
     gene            242..970
                     /locus_tag="VM692_06990"
     CDS             242..970
                     /locus_tag="VM692_06990"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUQ51949.1"
                     /translation="MRQRPTLTRRTLLSTVGLALGGAALLDARFAAAQEPLKIGIIGT
                     GRIGGALARHWVKAGHEVFMSSRHPEELEALAKELGPKAHVGQPREAAAFGSVVLVSV
                     PYGSMPQIGTDFAAELAGKVIIDTSNPVANRDGPQVAEWQRKGAGVSTAELLKNNRVV
                     RAFNCIPAASLADRSNRQPERIAIPIGGDDAAAIAIAERLIRDAGFDPVMIGTLAQSR
                     QFDLGQPLAQGNLSAAELRERMPR"
     gene            complement(1004..1282)
                     /locus_tag="VM692_06995"
     CDS             complement(1004..1282)
                     /locus_tag="VM692_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51950.1"
                     /translation="MARVKPSIRPTETYTEPNFLRVYTAEQPEPEGQRLATIEHLSYS
                     AANGSGRSGWSCTTIVDCEAMSKDDAMFIARGYAVEHNVPVIYECHAE"
     gene            1553..2893
                     /locus_tag="VM692_07000"
     CDS             1553..2893
                     /locus_tag="VM692_07000"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HUQ51951.1"
                     /translation="MSDVQEEDSGSGKKQSARIRFIEQFLRQGQQSRLAGLLLGQLDA
                     FNRIATTFGHEQCETFCAGYSERLRDILPPRTPIIRLSERRFAVLVDCDSMSKVMDVA
                     VALTEDNPPQLEVGGDHFLVDVTLGIAVYPSHADDAATLFRRAELALKEAREKELAYD
                     IYRPDATQQQAALWKFESELQSAVARGQLEVYFQPKVEIASHRVCGVEALVRWRTESG
                     NFVPASDFIPLAESNGAIVPLTWLVFDQIIEQLKKWETIPAPFSVAVNVSPQILTHAE
                     FLTRVKALKLALDQRKMNLTIELTEDSLVQSDAKTLSSIERLRKLGVDLAIDDFGKGY
                     SSLTYLKQIPAAEIKIDKRFIGTVAVDETDKQIVKTVIALAHALGMRVVAEGVDSAEA
                     IQAVGALECEMAQGFYIGRPMRGDLVVEWIEHYAATTTMRKAPVIREWPEHARV"
     gene            2970..4259
                     /locus_tag="VM692_07005"
     CDS             2970..4259
                     /locus_tag="VM692_07005"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HUQ51952.1"
                     /translation="MRLLLDFVYLIACIILSPWLVYRLCTTERRDFARRFGIGLGASL
                     PSCIWLHGSSAGEVTVLRPLVAALEREHPDLPLVISAFTATGLETARKLFGKHRVVPL
                     PFDLSFVVARCLRRFNPRLLVVAESEFWPNLIDVAHRRGVPAMLVNGKMSAKSFRVHA
                     RTRLIPRALEKLAVLAVQTEEHAQRLRALGVAAARLHVTGNMKYDLTPPRGGADQGRE
                     LRAALGFAAADVVVIGGSLHDQEDEALLDAYAAARAAVADTALVIVPRYPADAEAVEH
                     RAKARGFTVTRKTAIDSGAARPPGKSGVLVVDTVGELARLYAIADLAFVGGSLFYRGA
                     NKGGHNLMEPAIFGVAVLFGPYNFSFKETVDDLLAADAGRLVRDAAELRAAVVALVED
                     RDGRRALGSRALRVVDQGRGATARNYALLAQLLHARL"
     gene            4322..>5042
                     /gene="kdsA"
                     /locus_tag="VM692_07010"
     CDS             4322..>5042
                     /gene="kdsA"
                     /locus_tag="VM692_07010"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HUQ51953.1"
                     /translation="MTDSKAKKHVFIGDIDCGSDQLFLIAGPCVIEEESLMMRTAEKL
                     KGVAERLNVGLIFKSSFSKDNRSSLEYYQGPGMEAGLEMLAKIKRTFELPILTDIHYP
                     YQAAPAAEVCDVIQIPAYLCMQSELVVAAAKTGAVVNIKHGQFLAPENMAKPVKKIED
                     SGNDRIIVTERGYTFGYNDLVVDPRSFYLLNQIGYPVVFDAGHSIRKYGIPSKDPRGS
                     ARQFLTTLARSAVAAGVDGFFI"
BASE COUNT          829 a         1671 c         1649 g          893 t
ORIGIN      
        1 gccgcgcgtc atgaacggct tctcggatct gatcctcgag ctgttcggtc ccgatcgcgg
       61 cgcgcattcg cgcagcgccg tcggcatggc cgagttgcca ttcgatctac ccgtcgagat
      121 cgaggccgag ctcgagatcg agccgtagtc gttgcgacgg tcaagatttg tcgagccttc
      181 tgtcggctgc ccggcgagca cgggctatga tcctcccgat tcggaccttc aggggaatgc
      241 catgcgccaa cgaccgacgc tgactcgccg cacgttgctc tcgaccgtcg gcctcgcgct
      301 gggcggcgcc gcgctgctcg acgcgcggtt cgcggcggcg caggagccgc tgaagatcgg
      361 catcatcggc acgggccgca tcggcggcgc gctcgcgcgc cattgggtca aggccgggca
      421 cgaggtcttc atgtcgtcgc gccatcccga ggagctcgag gcgctcgcca aggagcttgg
      481 gccgaaagcg cacgtcggcc agccgcgcga ggccgcggct ttcggctccg tcgtgctggt
      541 ttcggtcccc tacggctcga tgccgcaaat cggcaccgat ttcgccgcgg agctcgccgg
      601 caaggtcatc atcgacacga gcaatcccgt cgcgaaccgc gacggcccgc aagtggccga
      661 gtggcagcgc aagggcgccg gcgtgtcgac cgcggagctg ctgaagaaca atcgcgtcgt
      721 gcgggcgttc aactgcattc cggccgcgtc gctcgccgac cggtccaatc gtcagcctga
      781 gcgcatcgcg attccgatcg gcggcgacga tgccgccgcg atcgcgatcg ccgagcggct
      841 cattcgcgac gcgggcttcg atcccgtgat gatcggcacg ctcgcgcagt cgcggcagtt
      901 cgacttgggc cagccgctcg cgcaaggcaa cttgagcgcg gcagagcttc gcgagaggat
      961 gccgcgctag cgacggcgtc ggcagctcgt cagaaccgcg ccgtcactcg gcgtggcact
     1021 cgtagatcac ggggacgttg tgctcgacgg cgtagccgcg cgcgatgaac atcgcgtcgt
     1081 ccttcgacat cgcctcgcaa tccacgatcg tcgtgcagct ccaacccgag cggccgctgc
     1141 cgttcgcggc gctgtacgac aagtgctcga tcgtagcgag ccgctgaccc tcgggctccg
     1201 gctgctcggc cgtgtagacg cgcaggaagt tcggctcggt gtaagtctcg gtgggcctga
     1261 tgctcggctt cactctggcc atggcttgtc cccgctcccc gcgttcccgt ggcgtactct
     1321 aggcttcgaa gcaagcggcg tgccagccgt gtcgttgcgc catacgcgac gttgcgggcc
     1381 gcgttatgtc gaagctgatc tgacgcgttc cgtcagaagt ggacgcgccg agaggggttg
     1441 gcgaccgact tggcgcacgc tgcccatcgt gattcctctc acagcgttaa gcggccgact
     1501 ctgctgaaat tgccactccc gtcgttagca ggatgctact ggggaccccc gagtgagcga
     1561 cgttcaagaa gaagacagcg gctccggcaa gaagcagagc gcgcgaattc gtttcatcga
     1621 gcagttcttg cggcagggcc agcagagtcg cctcgccggc ttgctgctcg ggcagctcga
     1681 cgcattcaat cgcatcgcga cgacgttcgg tcacgagcaa tgcgagacgt tttgcgccgg
     1741 ttattcggag cgcttgcgcg acattctgcc gccgcgcact ccgatcatcc gtctctcgga
     1801 acgccgcttc gccgtgctcg tcgattgcga ctcgatgtcg aaagtcatgg acgtcgccgt
     1861 cgcgttgacg gaggacaatc cgccgcagct cgaggtcggc ggcgatcatt tcctcgtcga
     1921 cgtcacgctc ggcatcgcgg tctacccgtc gcacgccgac gatgcagcga cgctgttccg
     1981 ccgtgccgag ctcgcgttga aggaagcgcg tgagaaagag ctcgcgtacg acatctatcg
     2041 ccccgacgcg acgcagcagc aagcggcgct ctggaaattc gagtccgagc tgcaaagcgc
     2101 cgtggcgcgc ggccagctcg aggtctactt tcaaccgaaa gtcgagatcg cttcgcaccg
     2161 agtctgcggc gtcgaagcgc tcgtgcgctg gcgcacggag tcgggcaact tcgtgccggc
     2221 tagcgacttc attccgctgg ccgagagcaa cggcgccatc gtgccgctga cgtggctcgt
     2281 gttcgatcag atcatcgagc agctcaagaa gtgggaaacc atcccggcgc cattctcggt
     2341 cgccgtcaac gtctcgccgc aaatcctcac gcatgcggag ttcctgaccc gtgtcaaagc
     2401 gctgaagctg gcgctcgacc aacgcaaaat gaacctgacg atcgagctta ccgaggacag
     2461 cctcgtacaa agcgatgcca agacgctctc gagcattgaa cgtctgcgca agctcggcgt
     2521 cgatctcgcg atcgacgact tcggcaaagg ctattcgtcg ttgacgtact tgaagcagat
     2581 cccggccgca gagatcaaaa tcgacaaacg tttcatcggc acggttgccg tcgatgagac
     2641 cgacaagcag atcgtcaaga ccgtcatcgc gctcgcgcat gcgctcggca tgcgcgtcgt
     2701 cgccgaaggc gtggatagcg ccgaagcgat tcaggccgtc ggcgccctcg agtgcgagat
     2761 ggcgcagggc ttctacatcg gccggccgat gcgcggcgat ctcgtcgtcg agtggatcga
     2821 gcactacgcg gccacgacga cgatgcgcaa ggcgcccgtc atccgggaat ggccggagca
     2881 cgctcgcgtc taatcgccgc ttcgcgtcgc cagtggagta aactggcgcg ccggtgcccg
     2941 ccgtaagtag cgtgccgcca agtgggccga tgcgactgct gctcgacttc gtttacctga
     3001 tcgcttgcat cattctgtcg ccgtggctcg tctatcggct gtgcacgacg gagcggcgtg
     3061 actttgcacg gcgtttcggc atcgggctcg gcgcgtcgct gccgtcctgc atctggctgc
     3121 acggctcgtc ggccggcgag gtgaccgtct tgcggccgct cgtcgcggct ctcgagcgcg
     3181 agcaccccga tctgccgctc gtgatctctg cgttcacggc gaccgggctc gagacggcgc
     3241 gcaaactgtt cgggaagcac cgagtcgtgc cgttgccctt cgacctgtcg ttcgtcgtcg
     3301 cgcgctgctt gaggcgcttc aacccgcggt tactcgtcgt cgcggagtcg gagttctggc
     3361 cgaacctcat cgacgtcgcg caccggcgcg gcgtgccggc gatgctcgtc aacggcaaga
     3421 tgtcggcgaa gtcgttccgc gttcatgcgc gcacgcggct gatcccgcgc gcgctcgaga
     3481 agctcgccgt gctggcagtg cagaccgagg agcacgcgca gcggttgcgg gcgctgggcg
     3541 tcgccgcggc gcgtctgcac gtgaccggca acatgaagta cgacctcacg ccgccgcgcg
     3601 gcggcgcgga tcagggccgc gagctgagag cagccctcgg cttcgccgcc gccgacgtcg
     3661 tcgtcatcgg cggcagcctg cacgaccagg aagacgaggc gttgctcgat gcttacgccg
     3721 cggcgcgcgc cgctgtcgcg gacactgcgc tcgtcatcgt gccgcgctac ccggccgatg
     3781 cagaggccgt cgagcaccgt gccaaggcgc gcggttttac cgttacgcgc aagacggcca
     3841 tcgactcggg tgcggcgcgg ccgcccggca agagcggcgt gctcgtcgtc gacacggtcg
     3901 gcgagctcgc ccgtttgtac gcgattgcgg acctcgcgtt cgtcggcggc agcctgttct
     3961 atcgcggcgc aaacaagggc ggacacaatc tcatggagcc ggcgatcttc ggcgtggccg
     4021 tgctgttcgg cccgtacaat ttcagcttca aggaaaccgt cgacgatctg ctcgcggccg
     4081 acgcgggccg actcgtgcgc gacgccgcag agttgcgtgc ggcagtcgtc gcgctcgtcg
     4141 aggatcgcga cggacggcgg gcgcttggat cgagagcgct gcgcgtcgtc gatcaggggc
     4201 gcggcgcaac cgcgcgcaat tacgcgctgc tcgcgcagct gttgcacgcg cggctttgag
     4261 tcttgccacg cggcgaccct ggccccaaaa tgccgcggta ctttaggcac ttgggcgctc
     4321 aatgaccgac tcaaaagcga agaagcacgt cttcatcggc gacatcgact gcggctcgga
     4381 tcagttgttc ctgattgccg ggccgtgcgt catcgaggaa gagtcgctga tgatgcggac
     4441 ggcagaaaag ctcaagggcg tcgccgagcg actgaacgtc gggctcatct tcaagtcgtc
     4501 gttctcgaag gacaaccgca gctcgctcga gtactaccaa ggccccggca tggaagcggg
     4561 cctcgagatg ctcgccaaga tcaaacgcac gttcgagctg ccgatcctca cggacattca
     4621 ttatccgtat caggcggccc cggcagccga ggtttgcgac gtcattcaga ttccggcgta
     4681 cctctgcatg caatcggagc tcgtcgtcgc ggcggccaag acgggcgccg tggtcaacat
     4741 caagcacggc cagttcctgg cgcccgagaa catggccaag cccgtcaaga agatcgaaga
     4801 ctccggcaac gatcgcatca tcgtcaccga gcgcggctac acgttcggct acaacgatct
     4861 cgtcgtcgat ccgcgcagct tctacttgct gaatcagatc ggctatcccg tcgtgttcga
     4921 cgcgggccac tcgattcgca agtacggcat tccgagcaag gacccgcgcg gcagcgcgcg
     4981 tcagttcctg acgacgttgg cgcgctccgc cgttgcggcc ggcgtcgacg gcttcttcat
     5041 cg
//
LOCUS       DATLEE010000327         2711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4842765, whole genome shotgun sequence.
ACCESSION   DATLEE010000327 DATLEE010000000
VERSION     DATLEE010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4842765"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..270
                     /locus_tag="VM692_07015"
     CDS             64..270
                     /locus_tag="VM692_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51954.1"
                     /translation="MLAFVGWLLLFVLCWPLALLALLLWPILWLISLPFRLIGITFEA
                     LFAFLRALLFLPARILGHRDREPR"
     gene            complement(282..1208)
                     /locus_tag="VM692_07020"
     CDS             complement(282..1208)
                     /locus_tag="VM692_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51955.1"
                     /translation="MRSWSKVVALAALAAGATSSAHAQSACDRACLTGFVDAYFAALF
                     GNDAARLRQAARARITDNGTEKKLADTFWDGATEAVYRFDIVNTDRGDTGTQTVIRNA
                     DGSKTMHMVRLKVLNGAITEIETIRANQGEADRLWDPDRLTEVSPDLTQSIRIAERDS
                     YYDLIGTAESYWRAFQTNGTPEYRPARLLPDARRFENGLQTTGLVRNGEYQSAQRGFD
                     EGRFLNRNIWDRRYPVVDEERGIVLSIVRFGIKAGATSQSNVTSNDRLVAEFFAVKRG
                     YIQEIQAVLFNLPDAQPTGWPRDTGPGRGAAH"
     gene            complement(1217..2491)
                     /locus_tag="VM692_07025"
     CDS             complement(1217..2491)
                     /locus_tag="VM692_07025"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ51956.1"
                     /translation="MKATSKLAASVRAAAGLAAVATLTISTATFAQPPGPAGEQFKVV
                     TVAEGLRNPWSMAWLPNGDMLVTERVGTLRIIRGGKLLPTEVPGVPAVRALGQGGMQE
                     VAVHPNFAQNHYIYLSYAKPRDGTATTCRGPAPANQPAGQPPCVEGTTALMRAKLEGD
                     KLVDAKEIFEAKAWNDAPGHFGARIQFDGQGHLFMSVGDRMAGLFPRRPDGAMDPNLE
                     GHPAQQLSSHQGKILRLNDDGTVPQDNPFVGRAGALPEIWSMGHRNPQGLFFDRDTGI
                     LWETEHGPQGGDELNVIEKGKNYGWPVIGYGANYTLGTEIHASRNKEGMEQPKAFFVP
                     SIGISGLMKYTGDQFPNWKGNLFTGGMAGNYRQLVRFSINDKVVTNRESLLPREYRIR
                     DLRQGPDGFVYIAVDNQFPGQPSNIIRLEPNK"
BASE COUNT          397 a          867 c          905 g          542 t
ORIGIN      
        1 agttgcgccg cgccgcgggg gtgctagggt aaggtcctcg cgggttcgct cgtcgggtgc
       61 aacgtgctgg cgttcgtcgg ttggctgctg ctgttcgtgc tgtgctggcc gctcgcgctg
      121 ctggcgctgc tgctctggcc gatcctctgg ctgatctcgc tgccgttcag attgatcggc
      181 atcaccttcg aagcgctgtt cgcgtttctg cgcgcgctgc tgttcctacc ggcgaggatt
      241 ctcggccacc gggatcgcga gccgcgctga ttagggcgcg ctcagtgcgc ggcaccccgg
      301 cccggacccg tgtcgcgcgg ccagcccgtc ggctgcgcgt ccggcaagtt gaacagcacg
      361 gcttggattt cttgaatgta cccgcgcttg acggcgaaga actcggcgac gagccgatcg
      421 ttcgacgtga cgttgctctg gctcgtcgcg ccggccttga tgccgaagcg cacgatcgac
      481 aacacgatgc cacgctcctc gtcgacgacg gggtagcggc ggtcccagat gttgcgattc
      541 aagaagcggc cttcgtcgaa gccgcgttgc gctgactgat attcgccgtt gcggacgagg
      601 cccgtcgtct gtaagccgtt ctcaaagcgg cgcgcgtcgg gcagcaagcg ggcagggcga
      661 tactcgggtg tgccgttcgt ctggaatgcg cgccaatagc tctcggcggt tccgatcagg
      721 tcgtaatacg aatcgcgctc ggcgatacgg atcgattgcg ttaggtccgg tgagacctcg
      781 gttagccgat cgggatccca aagccgatcg gcctcgcctt gattggcgcg aatcgtctcg
      841 atctccgtga tcgcgccgtt cagcactttg agacgcacca tgtgcatcgt cttgctgccg
      901 tcggcgttgc gaatgacggt ctgcgttccc gtgtcgccgc ggtccgtgtt gacgatgtcg
      961 aagcggtaga ccgcctccgt cgcgccatcc cagaaagtgt cggcgagttt tttctcggtg
     1021 ccgttgtccg tgatgcgtgc gcgtgccgct tgccgcaagc gcgcagcgtc gttgccgaat
     1081 agcgcggcga agtacgcgtc gacgaagccc gtgaggcagg cgcgatcgca ggcggattgc
     1141 gcgtgcgccg acgacgttgc gcccgccgcg agtgcggcga gcgcaacgac tttcgaccag
     1201 cttcgcacgc gaagccttac ttgttcggct ccagccgaat gatgttgctc ggctgccccg
     1261 ggaactgatt gtcgacggcg atgtagacga agccgtcggg gccctgacgc aggtcgcgga
     1321 tgcggtactc acgcggcaac agcgactcgc gattcgtgac gaccttgtcg ttgatcgaga
     1381 accgcacgag ctgccggtag ttgccggcca tgccgcccgt gaacaggttg cccttccagt
     1441 tcgggaactg gtcgcccgtg tacttcatga ggcccgagat gccgatcgac ggcacgaaga
     1501 acgccttcgg ttgctccatg ccttccttgt tgcggctcgc atggatttcg gtgccgagcg
     1561 tgtagttcgc gccgtagccg atgacgggcc agccgtagtt cttgcccttc tcgatgacgt
     1621 tgagctcgtc gccgccctgc gggccgtgct cggtctccca gagaatgccc gtgtcgcggt
     1681 cgaagaacag gccctgcggg ttgcggtggc ccatgctcca aatctcgggc agcgcgcccg
     1741 cgcggccgac gaacggattg tcctgcggca cggtaccgtc gtcgttcaag cgcaggatct
     1801 tgccctggtg gctgctcaac tgctgcgccg gatggccttc gaggtttgga tccatcgcgc
     1861 cgtcggggcg ccgcgggaac agcccggcca tgcggtcgcc gaccgacatg aagagatgcc
     1921 cctggccgtc gaactggata cgcgcgccga agtggcccgg cgcgtcgttc cacgccttcg
     1981 cttcgaagat ctccttcgcg tcgacgagct tgtcgccctc gagcttggcg cgcatcagcg
     2041 ccgtcgtgcc ttccacgcaa ggcggctggc ccgcgggttg gttcgccgga gcggggccgc
     2101 ggcacgtcgt tgccgtgccg tcacgcggct tcgcgtagct caaatagatg tagtggttct
     2161 gcgcgaagtt cgggtgcacc gcaacttcct gcatgccgcc ttggcccagc gcacgcacgg
     2221 ccggcactcc cggcacctcg gtcggcaaca gcttgccgcc gcggatgatg cgcagcgtgc
     2281 cgacgcgctc ggtcacgagc atgtcgccgt tcggcagcca cgccatgctc cacgggttac
     2341 gcaaaccctc ggcgacggtc acgactttga attgctcgcc ggccgggccc ggcggttgcg
     2401 cgaacgtcgc ggtggaaatc gtcaaggtcg cgacggcggc gagcccagcg gctgcacgga
     2461 ccgacgcagc aagtttcgat gttgccttca tggtgttcct taagttgagc gccccgcgct
     2521 tcgctaaaaa aagcagcgcg actgcgctgc ggatcttctt gtccgcccgg acgggcgtcg
     2581 gcctttatct tagcccgaac cgctccgttc gagcgacttt caatggatcg agaaatctcg
     2641 attccgagcg gttgacgctt ctgcgccgtt gggggaggct tgggacgacg ggggtaaaaa
     2701 catgagcaat c
//
LOCUS       DATLEE010000328         5493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_376584, whole genome shotgun sequence.
ACCESSION   DATLEE010000328 DATLEE010000000
VERSION     DATLEE010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376584"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VM692_07030"
     CDS             <1..309
                     /locus_tag="VM692_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ51957.1"
                     /translation="LRARAIATAIRDLVTRVDPARFQAAFGAGIEQLDALVAQQTVTI
                     AKLMEIAPPGTVNPTPSLYNVTMVLMAALLAIALVANALMAPVAAKHHMSDTALAQAR
                     "
     gene            310..1557
                     /locus_tag="VM692_07035"
     CDS             310..1557
                     /locus_tag="VM692_07035"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HUQ51958.1"
                     /translation="MVRYASRMLAVALLTLQVGCGPGSAPSAPNAATIITLDTHIDIP
                     LDYATDAVDPLTAANLQVNLTKMAAGGLDAGFFIVYVAQTARTAQNYAQAQADALTKF
                     GAIHRMAEQLYPERIALAYNADDVARIAASGKLVAVIGVENGYSLGPNLEMLDRYYEL
                     GARYVGLVHNGDNDLARSAQPRMELGDAAASAAADTGVTALGAMAIARLNRLGVMVDV
                     SHGSKQTALDAIRLSVAPVIASHSSVAAIAEHARNMDDETLRALAAKRGVVQIVAFDG
                     YLKKQPEERAVATRELREQIGLAANAQPTSLAPEQRAAYDQGMRDIEARWPSATVAQL
                     VDHIDYAVKLIGIDHVGIASDFDGGGGIVGWNDAGETANVTAELRARGYSEGDIAKIW
                     SGNLLRVWREVERTAADLKAAGR"
     gene            complement(1565..2569)
                     /locus_tag="VM692_07040"
     CDS             complement(1565..2569)
                     /locus_tag="VM692_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HUQ51959.1"
                     /translation="MSAQLQAKDYASSQEVRWCPGCGDYAVLKSVTKALADVGAVPEK
                     TVFVSGIGCSSRFPYYVETYGFHTIHGRAPAIATGLKLANPELDVWVVTGDGDGLSIG
                     GNHLLHALRRNVGFKILLFNNEIYGLTKGQYSPTSRPGTRSPSTPAGSFDTPISPGAF
                     AVGAGAKFIARAIDIDKVGLGSVLEAAHGFEGAAFVEIFQNCIVYNDGVFNSFTDRDK
                     AVAAQIRVEHGKPLVFGEGGSKGLGFDTKALALKVVDAKANPGEVLVHDETNRTLAQL
                     LLTMPQPEFPVALGVVLRQPGVAFEKAYYAAQPTKLKRTAKVADALRQTSIWRVPAAK
                     "
     gene            complement(2566..4440)
                     /locus_tag="VM692_07045"
     CDS             complement(2566..4440)
                     /locus_tag="VM692_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HUQ51960.1"
                     /translation="MDSQSVVVRFAGDSGDGVQLIGTQFVAATALAGDDFATFPDYPA
                     EIRAPAGTTFGVSAYQIHLGARPITTAGDAPQVLVAFNPAALKVNLPLIADGALIVLN
                     IDSFTSRGLAKAGYEKDPRQSGVLDGYQLIEVDITKHTMEATAKFGLSKSDAGRCKNF
                     WALGLVQWMFNRDVASIEDWLNKKFAKDATIRDANVAALRAGHAYGETAELTAAVPHV
                     GTQHAQFPPGEYRGVRGGEALALGLAAAGELADKPIIFCGYPITPASSLLHQLARYGN
                     MGVGTFQAEDEIAAVCAAIGASYGGAIGVTSSSGPGIALKTEAIGLAINAELPLVVVD
                     SQRGGPSTGLPTKTEQSDLYQAVYGRNADAPVPVIAARSPSDCFEVAIEAVRIAVRHM
                     TPVILLTDGYLANAAEPWRLPNIEEIPRIEVNTPGVRAEGQDVSSFIFGRNPETLGRP
                     WVAPGMPGLMYRVGGIEKDLRTGNISYDAQNHQAMTELRAAKVASVAKFIPPQKVEIG
                     PEKGALAVVGWGSTYGALYQAVRAMPPKERVAHIHIRYLSPLPENLGELLRGFDRILV
                     PEMNMGQLATLLRDKLGVETIQFNKVTGQPFLISELVQKIRSLLGVKPVVRAVRGERA
                     "
     gene            4693..5013
                     /locus_tag="VM692_07050"
     CDS             4693..5013
                     /locus_tag="VM692_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HUQ51961.1"
                     /translation="MPKVTYIQPDGTPKTVEAPAGTTLMEAAVDNDVVGIIAECGGAC
                     SCATCHVYVDPQWAPKLPPLDAQEDGMLGCVLDRRPTSRLSCQIVLKPALDGITVAVA
                     SEQI"
     gene            5104..5427
                     /gene="fdxA"
                     /locus_tag="VM692_07055"
     CDS             5104..5427
                     /gene="fdxA"
                     /locus_tag="VM692_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HUQ51962.1"
                     /translation="MTFVVTEACIKCKYTDCVEVCPVDCFHEGPNMLVIDPEECIDCT
                     LCEPECPVEAISSEDDVPAGQEQFLKLNAELSKGWPVITKAKAAPADADEWKDVKEKL
                     KHLER"
BASE COUNT          960 a         1870 c         1743 g          920 t
ORIGIN      
        1 ctgcgcgcgc gcgcgatcgc caccgcgatc cgcgatctcg tgacgcgagt cgaccccgca
       61 cgctttcaag cggcgttcgg cgcgggcatc gagcagctcg atgcgctcgt tgcgcagcag
      121 accgtgacga ttgcgaagct catggagatc gcgccgccgg gcaccgtcaa tccgacgccg
      181 agcctctaca acgtgacgat ggttttgatg gctgccttgc tcgcgatcgc gctcgtcgca
      241 aacgcgctga tggcgcccgt cgccgccaaa catcatatga gcgacacggc gctcgcgcag
      301 gcgcgttaga tggttcgcta cgcctcacgc atgctcgccg tcgcgctcct cacgctgcaa
      361 gtcggctgcg gccccggcag cgctccgtcc gcgccgaacg ccgcaacgat cattacgctc
      421 gacacgcaca tcgacattcc gctcgactac gcgacggacg ccgtcgatcc gctgacagcc
      481 gcgaatctgc aggtcaatct cacgaagatg gcggccggcg ggctcgatgc cggcttcttc
      541 atcgtctacg tcgcgcagac cgcgcggacg gcccaaaact acgcgcaggc gcaggccgat
      601 gcgttgacga aattcggcgc gattcaccgc atggccgagc agctctatcc ggaacgcatc
      661 gcgctcgcgt acaacgccga cgacgtcgcg cggatcgccg cgagcggcaa gctcgtggcc
      721 gtgatcggcg tcgagaacgg ctactcgctc ggccccaatc tcgaaatgct cgatcgctac
      781 tatgagctcg gcgcgcgcta cgtcggtctc gtccacaacg gcgacaacga cctcgcgcgt
      841 tcggctcaac ctcgcatgga gctcggcgac gcagcggctt cggcggccgc cgacacgggc
      901 gtcaccgcgc tcggcgctat ggccatcgcc cggctgaacc ggctcggcgt catggtcgac
      961 gtctcgcacg gctcgaaaca gacggcgctc gacgccatcc gtttgtcggt cgctcccgtg
     1021 atcgcgtcgc actcctccgt cgcagccatc gccgagcatg ctcgcaacat ggacgacgag
     1081 acgctgcgag cgctcgccgc gaaacgcggc gtcgtgcaaa tcgtcgcgtt cgacggctat
     1141 ttgaagaagc aacccgaaga aagggctgtc gcgacgcggg agctacgcga gcaaatcggc
     1201 ctcgcggcca acgcacagcc gacgtcgctc gcgcccgagc agcgagccgc ctacgaccag
     1261 ggtatgcgcg acatcgaagc gcgctggccc tcggcgaccg tcgcacagct cgtcgatcac
     1321 atcgattacg ccgtcaagct gatcggcatc gaccacgtcg gcatcgcgtc cgatttcgac
     1381 ggcggcggcg gcatcgtggg ctggaacgac gccggcgaaa cggcgaacgt cacggccgag
     1441 ttgcgggcgc gaggttacag cgaaggcgac atcgccaaga tctggagcgg caacttgctg
     1501 cgcgtctggc gcgaagtcga gcgcaccgcg gccgacctga aggccgcagg gcgctgaccg
     1561 gatactattt cgccgccggc acgcgccaga tgctcgtctg acgcagcgca tcggccacct
     1621 tggccgtgcg cttcagcttc gtcggctgtg ccgcgtaata cgccttctcg aacgcgactc
     1681 ccggctgacg caacacgacg ccgagcgcca ccgggaactc cggctgcggc atcgtcagca
     1741 acagctgggc cagcgtgcga ttcgtctcat cgtgcacgag gacctcgccg ggattggcct
     1801 tcgcatcgac gaccttcagc gccaacgcct tcgtgtcgaa cccgagcccc ttcgagccgc
     1861 cctcgccgaa caccagcggc ttgccgtgct cgacgcgaat ctgcgcagct acggccttgt
     1921 cgcggtccgt gaacgagttg aacacgccgt cgttgtagac gatgcagttc tggaagattt
     1981 cgacgaacgc cgcgccctcg aaaccgtgcg ccgcctcgag caccgagccc aagccgactt
     2041 tatcgatgtc gatcgcgcgc gcgatgaact tcgcgccggc gccgacggcg aaggctcccg
     2101 gagagatcgg ggtgtcgaac gagccggccg gcgtggacgg cgagcgcgtg ccgggacgcg
     2161 acgtcggcga gtattggccc ttcgtcaggc cgtagatctc gttgttgaac aacaggatct
     2221 tgaagccgac gttgcggcgc agcgcgtgca gcaaatgatt gccgccgatc gagagaccgt
     2281 cgccgtcgcc cgtgacgacc caaacgtcga gctcgggatt cgcgagcttg agccccgtcg
     2341 cgatcgccgg cgcgcggccg tggatcgtgt gaaagccgta ggtctcgacg taatacggga
     2401 accgcgacga gcagccgatg cccgagacga acaccgtctt ttcgggcacg gcgccgacgt
     2461 cggccagcgc cttcgtgacc gatttcagca cggcgtagtc gccgcagccg gggcaccaac
     2521 gcacttcttg cgatgacgcg tagtctttgg cttgcagttg cgcgctcatg ctcgctctcc
     2581 tcgcacggcc cgcacgacgg gtttgacgcc gagcaggctg cgaatctttt gcacgagctc
     2641 gctgatcaaa aacggctggc ccgtgacttt gttgaattga atcgtctcga cgccgagctt
     2701 gtcgcgcagc agcgtcgcga gctggcccat gttcatctcg ggcacgagga tgcggtcgaa
     2761 gccgcgcagc agctcgccca agttctcggg caacgggctc aagtaacgaa tatggatgtg
     2821 cgccacgcgc tctttgggcg gcatcgcgcg caccgcctga tacagcgcgc cgtaagtcga
     2881 gccccaaccg acgacggcaa gcgcgccctt ctcgggcccg atctcgacct tctgcggcgg
     2941 aatgaacttc gcgaccgacg cgaccttggc cgcgcgcagc tccgtcatcg cctgatggtt
     3001 ttgcgcgtcg tacgagatgt tgcccgtgcg caaatctttc tcgatgccgc cgacgcggta
     3061 catcaagccc ggcatgccag gcgcgaccca cggccggccc aacgtctcgg ggttgcggcc
     3121 gaagatgaac gagctgacgt cctggccctc ggcgcgcaca cccggcgtgt tgacctcgat
     3181 gcgcgggatc tcctcgatgt tcggcaagcg ccacggctcg gcggcattcg cgagatagcc
     3241 gtcggtcagc aggatgaccg gcgtcatgtg ccgcacggcg atgcgcacgg cttcgatcgc
     3301 gacctcgaag caatcgctgg ggctacgcgc cgcgatgact ggcaccggcg cgtcggcatt
     3361 gcggccgtac acggcttggt agagatccga ctgctcggtt ttcgtcggca aacccgtcga
     3421 cgggccgccg cgttgcgagt cgacgacgac gagcggcagc tccgcgttga tcgcgagccc
     3481 gatggcttcg gtcttcagcg cgatgccggg gcccgagctc gaggtgacgc cgatagcgcc
     3541 accgtacgag gcgccgattg ccgcgcagac cgccgcgatc tcgtcctcgg cctggaacgt
     3601 gccgacgccc atgttgccgt agcgcgcaag ctggtgcagc aacgacgacg ccggcgtgat
     3661 cggataaccg cagaaaatga tcggcttgtc ggcaagctcg ccggccgccg ctaacccaag
     3721 tgccagcgct tcgccgccgc gcacgccgcg atactcgccg ggcgggaact gcgcgtgctg
     3781 cgtgccgacg tgcggcactg ccgccgtgag ctcagccgtc tcgccatacg catggccggc
     3841 gcgcagcgcc gcaacgttcg catcgcgaat cgtcgcgtct ttcgcgaact tcttgtttag
     3901 ccagtcctcg atggacgcga cgtcacgatt gaacatccat tgcacgaggc cgagcgccca
     3961 aaaattcttg caacggcccg cgtcgctctt gctgagaccg aacttcgcgg tcgcttccat
     4021 cgtgtgcttc gtgatgtcga cttcgatcag ctgatagccg tcgagcacgc cgctctgccg
     4081 cgggtccttc tcgtagccgg ccttggcgag cccgcggctc gtgaagctgt cgatgttgag
     4141 gacgatcaac gcgccgtccg cgatcagcgg caggttgact ttgagggcag caggattgaa
     4201 cgccacgagc acctgcggcg cgtcgccggc cgtggtgatc ggccgcgcac cgaggtggat
     4261 ctgatatgcg gaaacgccga acgtggttcc ggccggcgcg cgaatctcgg ccgggtagtc
     4321 ggggaacgtc gcgaagtcgt cgcccgcaag cgcagtggcc gccacgaact gcgtcccgat
     4381 caactgcacg ccgtcgcccg agtcgccggc aaaccgcact actactgatt gagaatccat
     4441 gctgctacgt cactcgtctg acatttttta cagcggcgcg aaccgcggct gcgtccacca
     4501 tcacgtccct tcggctgcgc gcaagcgaca cgcgtcggtc agcacgcgcg gcggctcgag
     4561 tttttcgccg ggatctcctc gcggctcgat gtgcccaggg agggaattga ggcggtatta
     4621 tagccggcca gccgaagatt cagacaattc aaatgcataa gcatattgcc caggcactgc
     4681 gcgcctacgc ccatgcctaa ggtgacttat atccagccgg acggcacccc taaaaccgtc
     4741 gaggctccgg ccgggacgac gctcatggaa gcggccgtcg acaacgatgt cgtaggcatt
     4801 atcgcggagt gcggcggcgc ctgctcgtgc gcgacgtgcc atgtctacgt cgacccgcag
     4861 tgggcgccga agctgccccc gctcgacgcg caagaggacg gcatgctggg ttgcgttctg
     4921 gaccgccggc cgacgagtcg tttgagttgt cagatcgtgc tgaaaccggc gctcgacggc
     4981 atcaccgtcg cggtcgcttc ggagcaaata taggcgcgcg tcaggcttgg cccgatccgt
     5041 cgtttgtagc agaattccgc cccttcgaac cgcgcctcga gcgcacgcct tgtggagcaa
     5101 cgcatgacct tcgtcgtcac cgaagcctgt atcaagtgca agtacaccga ctgcgtcgag
     5161 gtgtgccccg tcgattgctt ccacgaaggc ccgaacatgc ttgtcatcga tcccgaggag
     5221 tgcatcgatt gcacgctctg cgagcccgag tgccccgtcg aggcgattag ctccgaggac
     5281 gacgtaccgg ccgggcaaga gcagttcctg aagctcaacg ccgagctgtc gaagggctgg
     5341 cccgtcatca cgaaggccaa ggccgccccc gccgacgcgg acgagtggaa ggacgtcaag
     5401 gaaaagctca agcacctcga gcgctgagcc gcctccttcg ctaggcctga cttttcaacg
     5461 gcagctagtt cagcaaaaac tcgatcgccc gct
//
LOCUS       DATLEE010000329         3111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3180533, whole genome shotgun sequence.
ACCESSION   DATLEE010000329 DATLEE010000000
VERSION     DATLEE010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3180533"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            642..>3111
                     /locus_tag="VM692_07060"
     CDS             642..>3111
                     /locus_tag="VM692_07060"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51963.1"
                     /translation="MSYETRNVLQACMRRGRAAVLACALVLIAACSGEGDGSVGIGSG
                     QDPDPVAPDFPIAYTKGPLFDEDDDLQVNTDLRDILRFAVGTDLYMRDRASPTATERN
                     ITRRFTEGTGDVSGVEISSDGKKILFAMRGPVDEDLALDDPDQPKWNIWEYVIATATL
                     RRLIVSDLTAQQGHDISPHYLPDGRIVFASTRQQTAKAILINDGKPQYDARDEDRQEP
                     AFVLHVMRDDGRDEFKQISFNQSSDYEPTVLDNGKVLFSRWDHAGSVNGIHLYQMNPD
                     GTELELLYGAESHLTGTNNGNVQFVSAREMADHRIMAILRPFDHPELGGAITIIDTPV
                     YVENTQAIATATGMTGPAQTAATPNQVRTDLRPSPGGRFSSAFPLWDGTGRVLTTWAI
                     CRLEEPDPARPTDPTAVIFVPCTPQRLAATNPAPVVAPPLYGVWMYDPTTQTQQPIVI
                     GEEGVIVSDVVAAQPRRTPASIPDKLLGVDFDADLAAEGAGIINIRSVYDVDGAASVN
                     IAAVADPVQTAPANRPARFLRIEKAVAIPDEDTVDLDNTAFGPNIQQGMREVIGYAPI
                     EPDGSVRVKVPANVALALSVLDANGRRTSARHQNWLQVVPGQELTCNGCHAPASNLSH
                     GRSTSFNAVYAGATSTGVAFPNTVGTFSPDAGETMAETRTRVSCQTDCAALEPSVDVL
                     YTDVWTNPAVATPAAPVSYLYSNLTTPAPASVNCIADWTPACRVIINYETHIHPLWKT
                     VRQVIDPMTMAVLADNTCAQSGCHAPVNAMNAPMVPAGQLDLTDGVSPDEADQFNSHR
                     ELLFADDRQVLMNGALVDEQVQIGV"
BASE COUNT          598 a          989 c         1004 g          520 t
ORIGIN      
        1 cgcggcttgc tcgcgcggtc ccgcgaaagc cagcgaggca ccgctcaaga tcagcgcgag
       61 agcaacgaac agacgccaca gcgcggtgtg gctgctcaga agcgcgaacg tcggcgcaaa
      121 gtgagtcctg ctaatcatcg cgatcgtccc gttgagtgat ggccagagtt cggtaagcgg
      181 gccgcactaa aacacaaaaa aaattgctgc gtaatgtggg aaatcacaga cgtctcatat
      241 tggcgacggt ttccgagtga cggtcttcga cgtgtaggct tctcgcgctg atcgagagca
      301 gcgcgcgagc gaactcggtc acaaaagagt gtagttaggt gaaatctcgt gcgacgacga
      361 gcgagtcgag tgtggcgaca tggagacgtc gttcacactt ttttttggcg aggtgatccg
      421 gaggagttct gtgatcacgt taacggaagg cacgaatgtc gatgcccatg attgccgtgg
      481 ccgacattac gtcgcatcgc gccgcaagcg ggcgcgagat tatgcaaggc aaccgcgagc
      541 gaaagaatcg ctcgctgccc tgcgttggag tgaatccgtt gacgcgtcga cagagctcgg
      601 aacggccctc ggtagcaccg attagtggag agggaatttc catgagctac gaaacacgga
      661 acgttttgca agcttgtatg cgccgcggcc gagcggccgt gctcgcgtgc gccctcgtgc
      721 tcatcgccgc gtgcagcggt gagggcgacg gcagcgtcgg catcggctcc ggtcaggatc
      781 ccgatccggt ggcgccggat ttcccgatcg cctacacgaa ggggccgttg ttcgacgagg
      841 acgacgacct gcaagtcaat accgacttgc gcgacatttt gcgcttcgcc gtcggcaccg
      901 atctttacat gcgcgatcgc gcgtcgccga cggcgaccga gcgcaacatt acgcgacgct
      961 tcacggaggg cacgggcgac gtcagcggcg tcgagatctc gagcgacggc aagaagattc
     1021 tgtttgcgat gcgcggcccc gtcgacgagg acctcgctct cgacgacccg gatcagccga
     1081 agtggaacat ctgggagtac gtgatcgcga ccgctacgtt gcgccggctc atcgtctccg
     1141 acctcacggc gcagcaaggc cacgacatct cgccgcacta tctgccggac gggcgcatcg
     1201 tgttcgcctc gacgcggcag caaacggcca aagcaatttt gatcaacgac ggcaagccgc
     1261 agtacgacgc gcgcgacgag gatcggcaag agccggcgtt cgtgctgcac gtcatgcgcg
     1321 acgacggccg cgacgagttc aagcaaatat cgttcaatca gagcagcgac tacgagccga
     1381 cggtgctcga caacggcaag gtgctcttca gccgttggga ccacgcgggc agcgtcaacg
     1441 gcattcatct atatcagatg aatcccgacg ggacggagct cgagttgctc tacggagccg
     1501 agagtcatct gacgggcacg aacaacggca acgtgcaatt cgttagtgca cgcgagatgg
     1561 ccgaccaccg catcatggcg atcttgcggc cgttcgacca tccggagctc ggcggggcga
     1621 tcacgatcat cgacacgcct gtctacgtcg agaacacgca ggcgatcgcg acggcgaccg
     1681 gcatgacggg gccggcgcag acggccgcga cgccgaatca agtgcgcacg gatctgcggc
     1741 cgtcgccggg cggccggttc agctcggcgt tcccgttgtg ggacggcacg ggccgcgtgc
     1801 tgacgacgtg ggcgatctgt cgcctcgaag agcccgatcc cgcgagaccg accgatccga
     1861 cggccgtcat tttcgtgcca tgcacgccgc agcgtttggc ggcgaccaat ccggcgcccg
     1921 tcgtggctcc gccgctctac ggcgtttgga tgtacgaccc gacgacgcag acgcagcagc
     1981 cgatcgtgat cggcgaggaa ggcgtcatcg tctcggacgt cgtcgccgcg cagccgcggc
     2041 gcacgccggc ttctattccc gacaagctgc tgggcgtcga tttcgatgcc gatctcgccg
     2101 ccgaaggcgc cggcatcatc aacatccgca gcgtctacga cgtcgacggc gcggcgagtg
     2161 tgaacatcgc cgccgttgcg gatcccgtcc aaactgcacc ggcgaatcgc ccggcgcgtt
     2221 tcctgcgcat cgagaaggct gtcgcgattc ccgacgagga caccgtcgat ctcgacaaca
     2281 cggcgttcgg cccgaatatc cagcaaggca tgcgcgaggt catcggctat gcgccgatcg
     2341 agcctgacgg ctccgtgcgc gtcaaagtgc cggccaacgt cgcgctcgct ttgagcgtgc
     2401 tcgatgcgaa cggccggcgc acgtcggcgc gccatcagaa ttggctgcaa gtcgtgcccg
     2461 ggcaagagct gacttgcaac ggttgccacg cgcctgcgag caacctgtcg cacggccgca
     2521 gcacgtcgtt caacgccgtg tacgcgggcg cgacgagcac cggcgtcgcg tttccgaaca
     2581 cggtcggcac gttctcgccc gatgccggcg agacgatggc cgagacgcgg acacgcgtga
     2641 gctgccaaac ggattgcgcc gcgctcgagc ccagcgtcga cgttctctat acggacgttt
     2701 ggacgaaccc cgcggttgcg acgccggccg cgccggtctc gtatctgtac tcgaacctga
     2761 cgacgccggc gccggccagc gtcaattgca tcgccgattg gacgcccgcg tgccgcgtca
     2821 tcatcaatta cgagacgcac atccacccgt tgtggaagac agtgcggcaa gtcatcgatc
     2881 cgatgaccat ggcagtgctc gcggacaaca cctgcgcgca gtccggttgc cacgccccgg
     2941 tcaacgcgat gaatgcgccg atggttcccg ccggtcagct cgacctcacg gacggcgtgt
     3001 cgcctgacga ggccgatcag ttcaattcgc accgcgagct gctgttcgcc gacgatcgtc
     3061 aggtcctcat gaacggcgcg ttggtcgacg agcaggtgca gatcggcgtc g
//
LOCUS       DATLEE010000330         7111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5596982, whole genome shotgun sequence.
ACCESSION   DATLEE010000330 DATLEE010000000
VERSION     DATLEE010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5596982"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1286
                     /locus_tag="VM692_07065"
     CDS             132..1286
                     /locus_tag="VM692_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HUQ51964.1"
                     /translation="MSSKLPFVMSAYAAALLALGGTASSQPPAPGAAPPPPVVSPEIA
                     ASRDVTLRLRAPSAERVDLVSGGDIPGVPMQGGLPLAKGADGVFSLKLPALASGAYRY
                     RFDVDGVATSDPSNPLTSESNSNAWSLFYVPGASFMDTQRVAHGSVAEVYYYSTVLNR
                     ARRMHVYTPPGYERNRDLYPVFYLLHGAFDGDDSWSTVGRAGFILDNLIAAGDARPMI
                     VVMPDGHTSRFGGGGAAGLNMAEFEREFTRDIKPHVETNYRIRGGRASTAIAGLSMGG
                     AQALDIAVGDLRSYAYVGVYSSGVFGIADNADWENSHRTQLDDKELKRGLELVWFSTG
                     KDDFLLDTTKQTVAMLKRHDFDVVYEESLGGHTWINWREYLTKFAPKLFH"
     gene            1356..2591
                     /locus_tag="VM692_07070"
     CDS             1356..2591
                     /locus_tag="VM692_07070"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ51965.1"
                     /translation="MLLDISPLRRHRDFRLVFIGQLFSSFGGFITYVALPVQVYELTQ
                     SSAIVGLIGVAQLVPLAIAALWGGAVADALDRRTLLLRCETLMACGSLALVGNSLLPN
                     PSVVALFAIAAFLSAVNGFHRPALEAITQKLVDRNELAAVSALTSLRGTTAAIAGPSL
                     AGISIAALGLPITYLLEVATFAVSVAALAAMRAMPPADTAKPAGLGTILEGLRYAGSR
                     PELIGTYAVDIVAMTFAMPLAIFPALAVQLGGTATVGFLYSAMSIGALLVTVFSAWTR
                     NFERRGAAVVVAAALWGVAIVGLGFSTSLPMAVACLVLAGAADMVSALFRMTIWNETI
                     PPALRGRMASIEQLSYMTGPLLGNARAGFMAEQFGLARSIVWGGIICVAGVALCIPAL
                     PRFWRYRNSPASEAAPSPE"
     gene            complement(2616..4178)
                     /gene="gltX"
                     /locus_tag="VM692_07075"
     CDS             complement(2616..4178)
                     /gene="gltX"
                     /locus_tag="VM692_07075"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HUQ51966.1"
                     /translation="MTVRTRFAPSPTGYLHIGGVRTALFNWLFSRQHGGQFLLRIDDT
                     DQQRNVAEALQPILDGFRWLGIEWDEGPEVGGPYEPYFQSQRQPAYRAAVARLVASGH
                     AYRDYATTEEIQAERTAAEKDKRAFLYSRKWMAATDEDAKRFEAEGRKGVVRLKMPRE
                     GQCRFHDLVRGDVAFDWANEQDHVVQRADGSCLYHMASAVDDHDFKITHVIRAIEHLS
                     NTPRQIFILQGLGYALPQFAHLPYVAEPGSQNKLSKRKIAQYLKHPDFKRLYDHALDI
                     AAKIGHATTPETFNPVVVDFYRVAGYLPDAIINYLLLLGWSLDDSREDFSRREMIELF
                     TLERVNKSPASFDPQKLWAFQDRHMRAVPIEAKVPAALQYLQRAGLVAAPPPATIGPY
                     LERVIAAAGDRIKTTGDVLQFREFFVADDELPVAGADFDKALKAPGAVALLREFAARL
                     EGASPFEPAALEQELKAFVAERNLKAGALIHPLRFAVTGRSVGLGLYDALAILGRERS
                     LARIRRAAAAAD"
     gene            4432..6825
                     /locus_tag="VM692_07080"
     CDS             4432..6825
                     /locus_tag="VM692_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ51967.1"
                     /translation="MSAHACAKSKLTLRYSPLAFAVIAAGLIPRGAFGQEAGEQNQLE
                     EIIVTATKQASGQDVSTVPISITAFDGEMIDRIGAKDLSALTAITPGVVLTNTQTFGL
                     SVTNIQIRGVASRTSEPTTGMYLGDTPFTTIGNNTNIGGSVAMPVVFDLERVEVLRGP
                     QGTLFGSTSEGGAIRLIPNAPSLTDTTSYMRLEGNSTAHGGSGYDFGYAMGGPIKEGK
                     LGYRFSTWYREDAGWINHCQPAVRVPGCLYVIEKDANSTTTRASRFALLWEPNDRLTI
                     EPSLQYQSVRNHDPTGYELQPSDPSQHIWNSDHSTDERANDPIATASVKLEWQLENLV
                     ATSVTSYINRRYKFHADYTQFQDMAFFGNPYPLTGLPDDYGLGDYGIYQHDLYEEFRV
                     ASAKTGSRWNWVAGIFLSDQTQYDYAHVVHPNLPDLVLANFGVPISRVLGSDPYLGKW
                     VAFNDVNTKSQEIALFGNVDYQITDKMRLTVAGRTTSYDQNITSFIAGPFNGGAQFFE
                     SDASGSPFTPKVSLSYQATDDTMYYVTAAKGFRIGGVNPQIDNAQPGCQAAKPGFIAR
                     GIKFDHQFEPDSLWSYELGVKTRLLDNRLSIDASAYHIDWKDIQNNLGITGCGFSMIF
                     NLGTASANGFDVGLQALVGDHVRLDANVGRTNARYTTTIPGLVTEGEQVSGPGSAVPP
                     WTATVGAEFNFRVGSRDAYAWVQDVYHSFNDGRFGSQTPADAATYNPLLPVNPETRMV
                     NLRVGLRLDKVDASLFVNNATDKHPLLAVGTSNARDLRLIGTTWRPRTVGINATFRY"
BASE COUNT         1230 a         2350 c         2288 g         1243 t
ORIGIN      
        1 cccccccccc cccccccgct ccggcccaaa aaaatcggcg cctcgcaaaa cagcacacct
       61 tagcggggtg ggcgccggga ggcgctcgct gtgctacttt gcccgcagcg gccttgtgag
      121 gggaggcacc gatgagcagc aagcttccgt tcgtgatgtc ggcgtatgca gcggcattgt
      181 tggcgctcgg cgggacggca tcgagccagc cgccggctcc gggcgccgcg ccaccgccgc
      241 ccgtcgtgtc gccggagatc gcggcgagcc gcgacgtgac attgcgcctg cgcgccccgt
      301 ctgcggagcg cgtcgatctc gtcagcggcg gtgacattcc gggcgtgccg atgcagggcg
      361 ggttgccgct tgcgaagggc gccgacggcg tcttctcgct gaagctgccg gcattggcgt
      421 ccggcgccta ccgttatcgc ttcgacgtcg acggcgtcgc gacgagcgac ccgagcaatc
      481 cgctgacgag cgagtcgaac agcaatgcct ggagcttgtt ctacgtgccg ggcgcgtcgt
      541 tcatggacac gcagcgcgtt gcacacggct cggtggccga ggtgtattac tactcgaccg
      601 ttctaaaccg agcgcggcgc atgcacgtct acacgccgcc cgggtacgag cgcaatcgcg
      661 acctctaccc cgtgttttat ctgctgcacg gcgcgttcga cggcgatgac tcgtggtcga
      721 cggtcggccg cgccggcttc atcctcgaca acctgatcgc cgccggcgac gcacgaccga
      781 tgatcgtcgt catgcccgac ggccacacga gccgtttcgg cggcgggggc gccgccggtc
      841 tcaacatggc ggaattcgag cgcgagttca cgcgcgacat caaaccgcac gtcgaaacga
      901 attaccgcat ccgcggcggc cgcgcgtcga cggcgatcgc gggcctgtcg atgggcggcg
      961 cacaggcgct cgacatcgca gtcggcgatc tcaggagcta cgcgtacgtc ggcgtctaca
     1021 gctcgggcgt cttcggcatc gccgacaacg ccgactggga gaacagccat cgcacgcagc
     1081 tcgacgacaa ggagctgaag cgcggcctcg agctcgtgtg gttctcgacg ggcaaggacg
     1141 acttcctgct cgacacgacg aagcagaccg tcgcgatgct caaaaggcac gacttcgacg
     1201 tcgtctacga ggaatcgcta ggcggccaca cgtggatcaa ctggcgcgaa tatctgacga
     1261 agttcgcgcc gaagttattc cactagacga attgccataa acgtgcacga gtagcccgcc
     1321 ctgccgacgc cctgcgcagg gccggcaaac cacccatgct gctagacatc tcgccgctgc
     1381 gccggcatcg cgatttccgg ctcgtcttca tcggccagct gttcagctcg ttcggcggct
     1441 tcatcaccta cgtcgcgttg ccggtccagg tgtacgagct cacgcagtcg tcggcgatcg
     1501 tagggctcat cggcgtcgca cagctcgtgc cgctcgccat cgcggcgctc tggggcggcg
     1561 ccgttgccga cgcgctcgat cgtcgcacct tgctgctgcg ctgcgagacg ctgatggcct
     1621 gcggctcgct cgcgctcgtc ggcaactcgc tattgccgaa cccgagcgtc gtcgccttgt
     1681 tcgccatcgc agcgttctta tcggcggtga acggttttca ccgcccggcg ctcgaggcaa
     1741 tcacgcagaa gctcgtggat cgcaacgagc tcgcggccgt ctcggcgctg acctcgctgc
     1801 gcggcacgac ggcggcgatc gccgggccgt cactggccgg catctcgatc gcagcgctcg
     1861 ggttgccgat aacgtatttg ctcgaggtgg cgacgttcgc cgtgtccgtc gccgcgctcg
     1921 ccgcgatgcg tgccatgccg ccggccgata ccgccaaacc tgcgggcctc ggcacgatcc
     1981 tcgagggcct gcgttatgcg ggcagccgtc ccgagctgat cgggacctac gccgtcgaca
     2041 tcgttgcgat gacgttcgcg atgccgctcg cgatctttcc ggcgctcgcc gtgcaattgg
     2101 gcggcacggc gacggtcggc ttcttgtatt cggcgatgag catcggcgct ctgctcgtca
     2161 cggtcttcag cgcttggacg cgcaacttcg agcggcgcgg cgccgccgtc gtcgtcgccg
     2221 cggctctgtg gggagtcgcg atcgtcgggc tcggcttttc gacttcgctg ccgatggcgg
     2281 tcgcgtgcct cgtgctcgcc ggagccgccg acatggtcag cgcgctgttt cgcatgacga
     2341 tctggaacga aacgattccg ccggcgctgc gcgggcgcat ggcgagcatc gagcagcttt
     2401 cgtacatgac ggggccgctg ctgggcaacg cgcgcgccgg cttcatggcg gaacagttcg
     2461 ggctcgcgcg ctcgatcgtc tggggcggaa tcatctgcgt cgccggcgtc gcgctgtgca
     2521 ttccggcatt gccgcgattc tggcgctatc gcaactcgcc ggcgtccgag gctgcaccga
     2581 gcccggagtg aagcagccag cgtagcggcc ggaggtcagt cggccgcggc cgccgcgcgg
     2641 cggatgcgcg caagcgagcg ctcgcggccg agaatggcaa gcgcatcgta gaggccgagc
     2701 ccgacggagc ggcccgtcac ggcaaaacgc aatgggtgga tgagcgctcc ggccttgaga
     2761 ttgcgctccg cgacgaacgc cttgagctct tgctcgagcg cggccggctc gaacgggctc
     2821 gcgccctcca accgtgccgc aaactcgcgc agcaatgcga cggctcccgg cgctttcaaa
     2881 gccttgtcga agtcggcgcc ggcaacgggc agctcgtcgt cggcgacgaa gaactcgcgg
     2941 aattgcagaa cgtcgcccgt cgtcttgatg cgatcgccgg ccgcggcgat gacgcgctcg
     3001 aggtacggcc cgatcgtcgc cggcggaggc gccgcgacga gcccggcacg ttgcagatat
     3061 tgcagcgccg cgggcacctt ggcctcgatc ggcacggcgc gcatgtgccg atcttggaac
     3121 gcccaaagct tttgcggatc gaagctcgcc ggcgacttgt tgacacgctc gagcgtgaac
     3181 agctcgatca tctcgcgccg cgagaaatct tcgcgcgaat cgtcgagcga ccagccgagc
     3241 agcaacaagt aattgatgat cgcgtctggc aagtagccgg cgacgcggta gaaatcgacg
     3301 acgacggggt tgaacgtctc cggcgtcgtc gcatgaccga tctttgccgc gatgtcgagt
     3361 gcgtggtcat agaggcgctt gaagtcggga tgcttcaagt attgcgcgat cttgcgcttg
     3421 ctgagcttgt tctgactgcc gggctccgcg acgtacggca agtgcgcgaa ctgcggcagc
     3481 gcgtaaccca atccttgcag gatgaagatc tgccgcggcg tgttcgatag atgctcgatc
     3541 gcgcggatta cgtgcgtgat tttgaagtcg tgatcgtcga cggcgctcgc catgtgatag
     3601 aggcaactgc cgtcggcgcg ctgcacgacg tgatcctgct cgttggccca atcgaacgcg
     3661 acgtcgccgc gcacgagatc gtggaagcgg cactggcctt cgcgcggcat cttgaggcgc
     3721 acgacgcctt tgcggccttc ggcctcgaac cgtttcgcat cctcgtcggt cgccgccatc
     3781 cacttgcggc tgtagaggaa cgcacgcttg tccttctcgg ccgccgtgcg ctcggcttga
     3841 atttcctcgg tcgtcgcgta gtcgcgatac gcgtggccgc tcgcgacgag gcgcgcgacg
     3901 gccgcgcgat acgcaggctg ccgttgcgac tggaagtacg gctcgtacgg cccgccgact
     3961 tccggcccct cgtcccactc gatgccgagc cagcggaagc cgtccaagat cggttgtagc
     4021 gcctcggcca cgttgcgctg ctggtcggta tcgtcgattc gcagcaggaa ttgcccgccg
     4081 tgctgccgcg agaacagcca attgaacagc gccgtgcgaa ccccgccgat gtgcaggtag
     4141 cccgtcgggc ttggcgcgaa ccgagtgcgt accgtcatgc cgtcctcgtc gaaaaggcgc
     4201 ccaatgtacg gggttttgca ggtcgagccc agccggcact gaacctcgac gagcgcaacg
     4261 gacgtaggcc gccgaggctc tctgcgcgcc ctcgcccgct tcgcgcagac gaacaacggc
     4321 cttcgcccct acgaaagaag cggcgcccaa ttgagcgcac attactgcca gccagcggct
     4381 attgccgttc cggcgagatc aagcgcgaac gccattgtcc tagggggaaa aatgtccgca
     4441 cacgcatgcg ccaagagcaa gctcacgttg cgttacagcc cgctcgcctt tgcggtgatc
     4501 gctgcgggac tgatcccccg cggcgcgttc ggccaggagg ccggcgagca gaaccagctc
     4561 gaagaaatca tcgtcaccgc cacgaaacaa gcctcgggcc aggacgtgtc gacggttccc
     4621 atcagtatca cggccttcga cggtgagatg atcgatagga tcggcgcgaa ggacttgagc
     4681 gcgctgaccg cgatcacgcc gggtgtcgtc ctcacgaata cccagacatt cggcctttcg
     4741 gtcaccaata tccagatccg cggcgtcgcg tcgagaacga gcgagccgac gacgggcatg
     4801 tatctcggcg ataccccctt caccacgatt ggcaacaaca ccaatatcgg cggctccgtc
     4861 gccatgccgg tcgtgttcga tctcgagcgc gtcgaggtgc tgcgcggccc gcaaggcact
     4921 ttgttcggct cgacgtcgga gggcggcgcc attcggctga ttccgaacgc tccgagcctc
     4981 accgacacca cgagctatat gcgtctcgag ggcaacagca ccgcgcacgg cggttccggc
     5041 tacgacttcg gctacgcgat gggcgggccg atcaaggaag gcaagctcgg ctaccggttc
     5101 agcacgtggt atcgcgaaga cgccggctgg atcaatcact gtcagccggc cgtcagagtg
     5161 ccggggtgtc tctacgtcat cgagaaggac gcgaattcga cgaccacgcg cgcaagccgt
     5221 ttcgcactgc tgtgggaacc gaacgacagg ctgacgatcg agccctcgct gcagtatcag
     5281 agcgtgcgca accacgaccc gacgggctac gagcttcagc cctcggaccc gtcgcagcac
     5341 atctggaact cggatcactc gaccgacgag cgggccaacg accccatcgc gaccgcgagc
     5401 gtgaagctcg agtggcagct cgagaatctc gtggcgacgt ccgtgacgtc ctacatcaac
     5461 cggcgctaca agttccatgc ggactacacg cagttccagg acatggcgtt cttcggcaat
     5521 ccgtatccgc tcacgggcct tcccgacgac tacggcctcg gcgattacgg catctaccag
     5581 cacgatctct atgaagagtt ccgcgtagcc tcggcaaaga cggggtcacg atggaactgg
     5641 gtcgcaggca tattcctgtc ggatcagacg cagtacgact acgcgcacgt ggtccatccg
     5701 aaccttccgg atctcgtcct cgccaacttc ggcgtgccca tcagccgagt tctgggttcg
     5761 gacccctacc tcggaaagtg ggtcgcgttc aacgacgtga acacgaagag ccaggaaatt
     5821 gcgctgttcg gcaatgtcga ttaccagatc accgacaaga tgcgcctgac cgtggcgggc
     5881 aggaccacga gctacgacca aaacatcacg agcttcatcg cgggcccgtt caacggcggc
     5941 gcgcaattct tcgagtccga tgccagcggc agcccgttca ctcccaaggt gtcgctgtcg
     6001 taccaggcca ccgacgatac gatgtactac gtcaccgcgg ccaagggatt caggatcggc
     6061 ggcgtgaacc cgcagatcga caatgcccaa ccgggctgtc aggcggcgaa gccgggcttt
     6121 atcgctcgcg gtatcaagtt cgaccatcag ttcgagcccg actcgctgtg gagctacgag
     6181 ctcggagtga agacgcgcct gctcgataac cgcctctcga tcgatgcgtc ggcgtaccac
     6241 atcgattgga aggacatcca gaacaaccta ggcatcacgg gctgcggctt ctcgatgatc
     6301 ttcaatctcg gtacggcgag cgccaatgga ttcgatgtcg gtctccaagc gctggtcggc
     6361 gatcatgtgc gactcgacgc gaacgtcggc cgtacgaacg cgcggtacac cacgacgatc
     6421 ccgggtctcg tgacggaagg tgagcaggtc agcggccccg gtagcgccgt gccgccgtgg
     6481 acggcgaccg tcggggccga gttcaacttc cgcgtcggca gccgtgacgc ctacgcgtgg
     6541 gtccaagacg tctaccacag cttcaacgac gggcggttcg ggtcgcagac gccggcggac
     6601 gccgcgacgt acaacccgtt gttgccggtc aatccggaga ctcgcatggt caacctgcga
     6661 gtcggcctgc ggctggacaa agtggacgcc tcgctgttcg tcaacaacgc gacggacaag
     6721 catccgctgt tggccgtcgg gacgtcgaac gcgagggatc tgcgcctgat cggtaccacg
     6781 tggcgcccgc gaaccgtggg gatcaacgca acgttccgtt actgattcgc caatcgcgaa
     6841 ttgttgttct cgggcgacgc ttgagtttga ggacgcccgg gaacaagcta ccccgagccg
     6901 cccgcgtacg cgagctgcga gaaggggaac ggccgtgtct gttgctgttg aaccacgtca
     6961 gcaaggtgcc gcgtcttggg atgagtttgc ctccgctgtc agctcgcagt tctctacacg
     7021 tttcgaattc acaccgctga cgcgctcgcc gttcgcaccg cagatggaat tccatgcggg
     7081 tgaaggcgtg gccgtctcgc atggagtgat g
//
LOCUS       DATLEE010000331         4403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10625903, whole genome shotgun sequence.
ACCESSION   DATLEE010000331 DATLEE010000000
VERSION     DATLEE010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10625903"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VM692_07085"
     CDS             complement(<1..574)
                     /locus_tag="VM692_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020398249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUQ51968.1"
                     /translation="MPADPRSVPPSLDDVARDKLAALERRQLRRRLVATARPDATAAV
                     QGEDELVSFSCNDYLGLSHHPEVVAASVEATRRFGAGAGSARLVNGNHPLYAELERKL
                     AALKGTEDAVVFGSGYLTNVGVIPALIGRSDLILLDELCHSCLLTGATLSGARVLEFR
                     HNDVAHCGELLRAERAAHRHCLVLTDGVFSM"
     gene            complement(637..3045)
                     /gene="lon"
                     /locus_tag="VM692_07090"
     CDS             complement(637..3045)
                     /gene="lon"
                     /locus_tag="VM692_07090"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HUQ51969.1"
                     /translation="MYDEDDDNQQLPQVTRETGPVTLPADVLLIVPVRNIVMFPGAVT
                     QVALTRDFSMRAAQEAVQHGYKLGIVLQKDPSVDAPGPDDLYKIGTTVSVVRYLRAPN
                     GVHHLICQGEQRFRTLDYLNGLPFLAARFDLIPEQLTSDANVEARAALLKQKAIEAIE
                     LLPQVAPELAPALQSIESPSALADMVAGLIDIKPSEKQMILEALKIRDRLDRVIEFLT
                     HRVEVLKLSREIGEQTQGKLDERQREYVLREQLKTIKKELGEDETESELDDLKQAIAD
                     AKMPEEAEKQAQKELRRLENMPEASMEHSMVRTYLDWLVALPWSKSDAETIDIAKARE
                     ILDADHYGLEKVKRRILEYLAVRKLNAAGRSPILCFVGPPGVGKTSLGQSIARAVGLK
                     FVRASLGGVHDEAEIRGHRRTYIGSLPGKIVQSLRKAGTNNPVFMLDEMDKLGVGFHG
                     DPSAALLEVLDPEQNSTFSDHYLDVPFDLSKVFFVATANVLDTIPGPLRDRMEIIELP
                     DYTQDEKLAIATRYLVARQLKANGLTEQQASITEAALRAVIEGYTREAGVRNLEREIG
                     ALLRHAAMKIAEGVAERVSIDAPDVHGILGPRHFENEVKLRTSVPGVATGLAWTPVGG
                     DILFIEATLVPGTGQLILTGQLGEVMKESAQAAVSLAKGRLDKLGVDRTVLEQNDLHI
                     HLPAGAIPKDGPSAGVAMFLAVASLLSGRTVRNDIAMTGEISLRGLVLPIGGLKEKSL
                     AALRAGIDTVLVPKRNEKDLEEIPEPARKQLKFVFLENIDDALAAAFDPPRGEAKRAH
                     RAVA"
     gene            complement(3149..>4403)
                     /locus_tag="VM692_07095"
     CDS             complement(3149..>4403)
                     /locus_tag="VM692_07095"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUQ51970.1"
                     /translation="GSAASPRVGRTPAFEYAVKMREFPAHARLDRKLEADEVPRSALA
                     RFAERLARFHGSLPPIRGIVADEIGKAALRNLDELGGQFSHNRALPLLRAWLKRQCAS
                     LAGVFAQRAATSHRECHGDLHLQNLLMRVGEIMAFDALEFDRALRDIDVVSEAAFLAM
                     DLRAHGRTDLAYAFLNRYLEAMGDYGGADVLPFYLVHRALIRAKVAAIKRAQGAADDH
                     GAQHYLATAVELTAATRPLLVVTHGLSGSGKTSVTDELVGRLPALRVRSDLERKRLHG
                     LEPAVRTGSALSAGLYAADATRRTYGVLADSADRLLRSGQTAVVDATFLRRSERLEFR
                     QIAAANAARFVILDCAASPRELRRRVAARASAGRDASEADLAVLEHQLVTHEPLDGAE
                     RRAAVTVDTEQPIRYERLAARLQKA"
BASE COUNT          691 a         1469 c         1506 g          737 t
ORIGIN      
        1 ccatgctgaa cacgccgtcg gtcagcacga ggcaatggcg atgtgcggcg cgctccgctc
       61 gcagcaactc gccgcagtgc gcgacgtcgt tgtgccggaa ctcgagcacg cgcgcgccgc
      121 tcagcgtggc ccccgtcagg aggcagctat gacagagctc gtccaacaag atgaggtcgc
      181 tgcgcccgat gagcgccggg atgacgccga cgttcgtcag gtagccgctg ccgaagacga
      241 ccgcgtcctc ggtgcctttg agcgcggcga gcttgcgctc gagctcggcg tacagcggat
      301 gattgccgtt cacgagccgc gcggaaccgg caccagcccc aaagcggcgc gtcgcttcga
      361 cgctggccgc gacgacctcg gggtggtgcg acaggccgag ataatcgttg cacgagaacg
      421 aaacgagctc gtcctcgccc tgcacggccg ccgtcgcatc gggcctagcc gtggcgacga
      481 ggcgccgccg cagttgccgg cgttcgagcg ccgcgagctt gtcgcgcgcg acgtcgtcga
      541 gcgagggcgg cacgctgcgc ggatcggctg gcaaaggaga tgctccggtc ggtgagacgg
      601 cattctcacc gaccggggct cgtggcggga agcgccctaa gcgacggcgc ggtgtgcgcg
      661 tttagcctcg ccgcgcggcg ggtcgaatgc tgcggcgagc gcgtcgtcga tgttctcgag
      721 aaacacgaac ttgagctgct tgcgcgcggg ctcgggaatt tcctcgaggt ccttctcgtt
      781 acgcttcggt accagcacgg tgtcgatgcc ggcgcgcagc gcggcaagcg acttctcctt
      841 gagaccgccg atcggcaaca ccaagccgcg caggctgatc tcgcccgtca tggcgatatc
      901 gttgcgcacg gttcggcccg acagcaaaga cgcaaccgcc aggaacatcg cgacgccggc
      961 gctcggcccg tccttcggga tcgcgcccgc cggcaagtgg atgtggaggt cgttctgctc
     1021 gagaacggtc cgatccacgc cgagcttgtc gagtcggccc ttggcaaggc tgaccgccgc
     1081 ctgcgcgctc tccttcatga cttcgccgag ctgacccgtc aggatcaact gccccgtgcc
     1141 ggggaccagc gttgcctcga tgaacagaat gtcgccgccc acgggcgtcc aggcgaggcc
     1201 cgtcgcgaca cccggcacgc tcgtgcgcag cttcacctcg ttctcgaagt gccgcggccc
     1261 gaggatgcca tgcacgtcgg gcgcgtcgat cgacacgcgt tcggcaacgc cttcggcgat
     1321 cttcatggcg gcatggcgca acagcgcgcc gatctcgcgc tcgagattgc gcacgccggc
     1381 ttcgcgcgta tagccctcga tgacggcgcg cagcgcagcc tcggtgatcg aggcctgctg
     1441 ctccgtgagg ccgttcgcct tcagctgccg cgcgaccaga taacgcgtcg cgatcgcaag
     1501 cttctcgtcc tgcgtgtagt cgggcagctc gatgatctcc atgcggtcgc gcaagggacc
     1561 gggaatcgtg tcgagcacgt tcgcggtcgc gacgaagaac accttgctca agtcgaacgg
     1621 cacgtcgagg tagtgatcgg agaacgtcga gttctgctcg ggatcgagca cctcgagcag
     1681 cgccgccgac gggtcgccat ggaagccgac accgagcttg tccatctcgt cgagcatgaa
     1741 cacggggttg ttggtcccgg ccttgcgcaa gctctgcacg attttgccgg gcagcgagcc
     1801 gatgtacgtg cggcggtggc cgcggatctc ggcctcgtcg tgcacgccac ccaagctcgc
     1861 gcgcacgaac ttgagaccga cggcgcgcgc gatgctctgc cccagcgacg tcttgccgac
     1921 gccgggcggg ccgacgaagc acaagatcgg gctgcgcccc gcggcgttca gcttgcgcac
     1981 ggcgaggtac tcgagaatgc ggcgcttgac cttctcgagg ccgtagtgat cggcgtctaa
     2041 gatctcgcgc gccttcgcga tgtcgatcgt ctccgcgtcg ctcttcgacc acggcaatgc
     2101 gacgagccag tcgaggtacg tccgcaccat cgagtgctcc atcgaggcct cgggcatgtt
     2161 ctcgagccgg cgcagctctt tctgagcctg cttctcggct tcttcgggca tcttcgcgtc
     2221 ggcgatcgcc tgcttgaggt cgtcgagctc ggactccgtt tcgtcctcgc cgagctcctt
     2281 cttgatcgtc ttcagctgct cgcgcagcac gtattcgcgt tgccgctcgt cgagcttgcc
     2341 ttgagtctgc tcgccgatct cgcgcgacag cttcaacacc tcgacgcggt gcgtcaaaaa
     2401 ctcgatgacg cgatcgaggc gatcgcgaat cttaagcgcc tcgagaatca tctgcttctc
     2461 ggacggcttg atgtcgatga ggcccgcaac catgtcggcg agcgcgctcg gcgactcgat
     2521 gctctgcaac gcgggcgcaa gctcgggcgc gacctgcggc agcagctcga tcgcctcgat
     2581 ggccttctgc ttcagcagcg cggcacgcgc ttcgacgttc gcatccgacg tgagctgctc
     2641 cggaatcagg tcgaaacgcg cggcgaggaa cggcagcccg ttcagatagt cgagcgtgcg
     2701 gaagcgctgc tcgccctggc agatcaagtg atggacgccg ttcggcgcgc gcagatagcg
     2761 gacgacgctg acggtcgtgc cgattttgta gagatcgtcg ggccccggcg cgtcgacgct
     2821 cgggtccttc tgcagcacga tgccgagctt gtaaccgtgc tgcacggcct cctgggccgc
     2881 acgcatcgaa aagtcgcgcg tcagcgccac ctgcgtcacg gcccccggga acatgacgat
     2941 attgcggacg gggacgatca gcagcacgtc cgccggcaac gtcacggggc cggtctcacg
     3001 ggtgacttgc ggcagctgct ggttgtcgtc gtcttcgtcg tacatgggga acctcgcggg
     3061 tgatccgagt gggctatctg gggccggcgc tatagacgtt caaggttcgt gccaatgcgc
     3121 cggggcggcg cgagggatcg cgagggaatt aagctttctg caaccgcgcc gcgaggcgct
     3181 catagcggat cggttgctcc gtatcgaccg taacggccgc gcgccgttcc gcgccgtcca
     3241 gaggctcgtg tgtcaccagc tggtgctcga gcacggccaa atctgcttcg gaggcgtcac
     3301 ggcccgcgct cgcgcgtgcg gccacgcgcc ggcgcagctc acgcggcgac gccgcacaat
     3361 cgagaatcac gaaacgcgcc gcattggccg ctgcgatctg gcgaaactcg aggcgctcgc
     3421 tacggcgcag gaacgtcgcg tcgacgacgg ccgtctggcc gctgcgcaac aagcgatccg
     3481 cgctgtcggc gagcacgccg taggtgcggc gcgtggcgtc cgcggcatag aggcctgcgc
     3541 tcaaggccga gcccgtgcgc acggcgggct cgaggccgtg cagccgtttg cgctcgagat
     3601 cggagcgcac tcgcagtgcg ggcaggcggc cgacgagctc gtcggtgacc gaagtcttgc
     3661 cgctgccgga caggccgtgc gtgacgacga gcagcggccg ggttgcggcc gtgagctcga
     3721 cggccgtcgc tagatagtgc tgcgcgccgt ggtcgtcggc ggcgccctgg gcacgcttga
     3781 tggccgccac cttcgcgcgg atcaaggcgc gatgcacgag atagaacggc agcacgtcgg
     3841 ctccgccgta gtcgcccatg gcctcgagat agcggtttag aaacgcgtag gcgagatccg
     3901 tgcggccgtg cgcgcgcagg tccatcgcga ggaacgcggc ttcgctgacg acgtcgatgt
     3961 cgcgcagcgc gcggtcgaac tcgagcgcgt cgaacgccat gatctcgccg acccgcatca
     4021 gcaggttctg caaatggaga tcgccgtggc attcgcgatg cgaagtcgcg gcacgctgtg
     4081 cgaatactcc tgcgaggctt gcgcattgcc gcttcagcca cgcccgtagc agcggtagtg
     4141 cgcggttgtg gctgaattgc ccgccgagct cgtcgaggtt gcgcaacgcg gctttaccga
     4201 tctcatcggc tacgatgccg cggatcggcg gcaggctgcc gtggaaacgt gcaagccgct
     4261 cggcgaaccg cgcgagagcg gaacgcggca cctcgtcggc ctcgagcttg cggtcgaggc
     4321 gcgcgtgcgc cggaaactcg cgcatcttga cggcgtactc gaacgccggc gtgcgcccga
     4381 cgcgcggcga ggcagccgag ccg
//
LOCUS       DATLEE010000332         2907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8386573, whole genome shotgun sequence.
ACCESSION   DATLEE010000332 DATLEE010000000
VERSION     DATLEE010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8386573"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VM692_07100"
     CDS             <1..978
                     /locus_tag="VM692_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ51971.1"
                     /translation="VAFLHARSVDRLEKHDVNDVEEAARLVARGVHDVVVVIDDTFAD
                     DFGTERAARVALVFDRSKSRAASRVGRVRGAIEAYGQQIGTLRLVANGADPSLLRPLV
                     VDELDVSTPASRSALMLGVLTYFLLFATLTGGLHLAIDTTAGERERKSLEPLLTLPVP
                     RSSLILGKMTATVCYMLASLTLTLLAFTLALRQLPLEQIGMSSTFGFATAGLAFLLLC
                     PFAPLGAALMTTVASFTKTYREAQTYLTFVLLVPTLPLAFATLLNIEPEAKLMWIPSL
                     SHHLLITTLIKDQPLDWTLVAVSVVSTLAFGTLLAWLAVRLYRREAILG"
     gene            1101..1679
                     /locus_tag="VM692_07105"
     CDS             1101..1679
                     /locus_tag="VM692_07105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HUQ51972.1"
                     /translation="MSETLTGRNAEAPSYDALTLSLTRPAFLRDLAEQARIAERSGNA
                     FCLLLVDVDHLKNINDCHGVAAGDAVLVGLADRCRRVVAEPAWHRSEYSFGRFDGGAL
                     MILARPCASSQAEMLAEALRFAVAEKPLSERISATVSVGVAQCRIGESIDELLARTER
                     VLHVAKQFGRDRVEVASSPPSRVERAKVVGLY"
     gene            1916..2224
                     /locus_tag="VM692_07110"
     CDS             1916..2224
                     /locus_tag="VM692_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51973.1"
                     /translation="MNDIEPVCVDGFGVFVVWRPGDYGRAPAVLYVGRGALRQEIADC
                     RRDPTMNNAQGLRITWAKVDPCDADGVAEYLYQQLRPLWGDVLRSRAAPQQVNLPMTA
                     "
BASE COUNT          484 a          930 c          939 g          554 t
ORIGIN      
        1 gtcgcgttcc tgcatgcgcg cagcgtcgac cggctcgaga agcacgacgt caacgacgtc
       61 gaggaagcgg cgcggctcgt cgcgcgcggc gtgcacgacg tggtagtcgt catcgacgac
      121 acgttcgccg acgatttcgg caccgagcga gccgcgcgcg tcgcgttggt gttcgaccgc
      181 tcgaagtcgc gggcggcaag ccgcgtgggg cgcgtgcgcg gtgcaatcga agcgtatggc
      241 cagcagatcg ggacgctgcg cctcgtcgcg aacggcgccg atccgagcct cttgcggccg
      301 ctcgtcgtcg acgagctcga cgtctcgacc cccgcgagcc gttcggcgct gatgctcggc
      361 gtgctcacgt atttcttgct gttcgcgacg ttgacgggcg gcttgcacct cgcgatcgac
      421 acaacagcgg gcgagcgcga gcgcaagtcg ctcgagccgc tgttgacgct ccccgtgccg
      481 cgctcgagct tgattctcgg caagatgacg gcgacggtct gctacatgct ggcgtcgctg
      541 acgctgacac tgctcgcctt cacgctggcg ctgcggcaat tgccgctcga gcagatcggc
      601 atgagctcga cgttcgggtt cgcgacggcc ggcctcgcat tcttgctgct atgcccgttc
      661 gcgccgctcg gcgcggcgtt gatgacgacg gtcgcgtcgt tcacgaagac ctatcgagag
      721 gcccagacgt atctgacgtt cgtgctgctc gtgccgacgc tgccgttggc gttcgcgacg
      781 ctgctgaaca tcgagcccga ggccaagctc atgtggatcc cgagcttgag ccaccacttg
      841 ctgatcacga cgctcatcaa ggaccaaccg ctcgactgga cgctggtggc ggtctccgtc
      901 gtcagcacgc tggcgttcgg cacgctgctt gcttggctcg ccgtgcgact ctatcggcgc
      961 gaagcaattc tcggttaacg gcgcgccgcg agttatgtaa acgcgctgct ctcgcttctg
     1021 cgagagcgtt cacgccgatc tcagcatcgg ggcggcaagc tgccacccgt tcgcccactc
     1081 gtgtcgagat cgactgaccg atgtctgaaa cgctgactgg ccgcaacgcg gaagcgccgt
     1141 cgtacgacgc cctgactctg agtctcacgc gtcccgcctt ccttcgagac ctcgccgagc
     1201 aagcgcggat cgcggagcgg agcggcaatg cgttctgctt actgctcgtc gacgtcgatc
     1261 acctgaagaa catcaacgat tgccacggcg tggccgccgg cgacgccgtg ctcgtcggcc
     1321 tggccgatcg ttgccgccgc gtcgttgccg agcccgcgtg gcaccgttcg gaatattcgt
     1381 tcgggcgctt cgacgggggc gcgctgatga tcctcgctcg cccgtgcgcc tcgagtcagg
     1441 ccgagatgct cgccgaggcg ctgcggttcg ccgtggccga gaagccgttg agtgaacgca
     1501 tcagcgccac cgtctcggtt ggtgtcgcac agtgccgcat tggcgagtcg atcgacgaac
     1561 tgctcgcgcg cacggagcgg gtactgcacg tcgcgaagca gttcggtcgc gaccgagtcg
     1621 aggtggcgag ttcgccgccg agccgagtcg aacgggccaa agtcgtcggc ctctactaac
     1681 gctttcccga gcggccccgg cgccgcggcg ggcgttgcga agtcgtcgca taacaagcac
     1741 ggatcttgca tatatctcca ctcagcggtg ggaaattgtc cttctcgccg ccaccaaccg
     1801 cgtagaacct aaggtcgcgg tggatgcgcc cgcaaaacaa agaagcgaag tttgagaatc
     1861 gggggatttc ttgcagcttc agtggtccaa gtgcgcagcc ggcgaatggt gtttcttgaa
     1921 tgacatcgag cccgtttgcg tcgacggctt cggcgtgttc gtcgtctggc ggcccggcga
     1981 ttatggacgc gcgcccgcgg tcttatacgt cggtcgcggc gcactgcgtc aggagatcgc
     2041 cgactgccgc cgcgacccta cgatgaacaa tgctcagggg ctgcggatca catgggcaaa
     2101 ggtggatccc tgcgacgccg acggcgtcgc tgagtacctc tatcagcaac ttcgtccgct
     2161 gtggggggat gtgcttcgct cacgggcggc accacagcaa gtgaacctgc cgatgacggc
     2221 gtgacggacg agcgaccgag ctggatcgcc gtgaccgtct cgacgagctt catgtactga
     2281 taccaggccc acggcggctc actgcccgtc gcgatgcggc tttcagccca tttccgtagc
     2341 gaggccaagc acttatctac gcctctgtcc atgactggga agggtccaag tttcatgcca
     2401 gcggccgccc acgcgaaaat cagtgagtta cgcgtccgga cgtgattggg cgcggcggtg
     2461 gcgcggtagc gccggttgca aatcttgcga cgggcgccgc tgactgccgc tgagcggcgt
     2521 cacttgccga tgcagaacgt caagtgcctt cgcaagtgcc tgttttcaaa agtcgggcga
     2581 aagcgagtcc caatgcgatg ggcacatctc tgggcacaat tgcgcagttt aaccaatcga
     2641 attgcgccgg cagtgcggac cgtcgtcaag acgctgaagg gttggctgcc gctctgctaa
     2701 gctgtcaacg ggtgtcgtag ccccttctgg ctgcgataga aaagattggt cgggccgcca
     2761 atcctgcata ggcgtacacc cgtgaatcgc accgatcagg ccaattccaa acaagctcta
     2821 tcgcccgtca atcgaattct caagccgctc gaccagtttc tccacgtgga gagcgcgagc
     2881 ggcgtggtgt tattgctcgc tgctgcc
//
LOCUS       DATLEE010000333         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5597987, whole genome shotgun sequence.
ACCESSION   DATLEE010000333 DATLEE010000000
VERSION     DATLEE010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5597987"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VM692_07115"
     CDS             <1..647
                     /locus_tag="VM692_07115"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HUQ51974.1"
                     /translation="LKDGQATWLREGDRVVSLRRQAGDLGYGGGVLLFELGPEQSLKE
                     VARADSADLDPANRWVLANYAETSFTAEGVAVRSERESTEAYGINPELLALSVVREDL
                     LDTPSLVRYIGYLLANDLDAHRYLVAYWSRMASVVSVVVMTLLALPFVFGSLRSAAAG
                     ARLLVGLIVGLSYYVSGEVLTSAGEVYRLDPVLIAWAPTAVLVLVTAVAFARIR"
     gene            complement(644..1051)
                     /locus_tag="VM692_07120"
     CDS             complement(644..1051)
                     /locus_tag="VM692_07120"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HUQ51975.1"
                     /translation="MWRRFAAFCYDMLLVAALEFCFTLIVLAVRLGAAVPPGTWWFPL
                     CLVAVAMAFFCGFWVHGGQTVGMRAWRIRVVHVAGGPLDWPRAAARFGALLVAALPAG
                     AGLWWSAFDRQNRGWHDRWTRTRVVRVPAPARR"
     gene            complement(1162..2733)
                     /locus_tag="VM692_07125"
     CDS             complement(1162..2733)
                     /locus_tag="VM692_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HUQ51976.1"
                     /translation="MVTEWHKEAEHALNRRDYRLAHELCLKILTAEPEHADALFLLGV
                     VAAEHRNFGKAVEIIDRALALAPAKPEYWAQRGRCLLSLQRPREGFEAAQRALELDPH
                     DALTLDTIGVVMTRAGAHTEAVEPFRRAVARDSTKAPYHYNLGASLQFSGALQDAELE
                     YREALRRDPTYHRAWSSLAQVARAPLTPADVATLERALEQPGLDADAELHLCHALAKQ
                     HEDEGRYDAAFRLLERGKRRKAAALGYGFAADGSLFAAAARLPAALAARAGDGHGHGS
                     HEPIFIVGMPRTGTTLVERILSSHPKVFSAGELTNFGLVLKRATATASNRVLDAETLD
                     ASARCDFAAVGEAYVESTRPRTGHKQRFIDKMPLNFFYAPLIRRCLPNAKIVCLRRNP
                     LDTCLSNYRQLFATSFSYYNYAYDLLDTGRYYAAFHALAALWRESLADNYLEVRYEDV
                     VDRTEREARRLVEFCGLPWDSRCLEFENNAAPVATASSAQVRQPIYRSGLERWRHYER
                     ELEPLRALLSSAGVL"
BASE COUNT          476 a          947 c         1006 g          458 t
ORIGIN      
        1 acctcaagga tgggcaggcg acctggctgc gcgagggcga ccgcgtcgtg agcttgcggc
       61 gtcaagcagg ggatctcggc tacggcggcg gcgtgttgtt gttcgagttg ggaccggaac
      121 aatcgttgaa ggaagtcgcg cgcgccgact ccgcggatct cgaccctgcg aaccgctggg
      181 tgctcgccaa ctacgcggaa acgtcattca ccgcggaagg cgtcgccgtg cgcagcgagc
      241 gtgagtcgac ggaggcgtat ggaatcaacc cggagctgct cgcgctatcg gtcgtccgcg
      301 aggacttgct cgatacgccg tcgctcgtgc gctacatcgg ctatctgctc gccaacgatc
      361 tcgacgctca tcgctatctc gtcgcctatt ggtcgcgcat ggcgagcgtc gtctccgtag
      421 tcgtgatgac gctgctcgca ttgccgttcg tgttcggcag cctgcgttcc gcagcagccg
      481 gggcgcgact gctcgtcggc ctgatcgtcg ggctgtcgta ttacgtctcc ggcgaggtgc
      541 tgacgagcgc cggcgaggtc tatcgtctcg acccagtgct tatcgcgtgg gcgccgacgg
      601 ccgtgctcgt gctcgtcacg gccgtggcgt tcgcccgcat tcgctaacga cgcgcaggcg
      661 ccggcacgcg cactactcgc gttcgcgtcc aacgatcgtg ccaaccccgg ttttggcggt
      721 caaaggcgct ccaccacaag cccgcgccgg ccggtaaggc cgcaacgagc agagcgccga
      781 agcgcgcggc ggcgcgcggc caatcgagcg ggccgccggc aacgtgcacc acgcgaatgc
      841 gccacgcgcg catgccgaca gtttgcccgc cgtgtaccca gaagccgcag aagaaggcca
      901 tcgcgaccgc gacgagacac aacgggaacc accaggtgcc gggcggcacg gcggcgccga
      961 gccgcacagc gagcacgatc agtgtgaagc agaactcgag cgccgcgacg agcagcatgt
     1021 cgtaacaaaa ggctgcgaat cgccgccaca tcggagccgg atcgagcgcg ccgctgggta
     1081 gtccctgcgg gggggcgagt gaagtagggt ctccgttcac agagagctcg tggtggcggg
     1141 cggcggcagt ctagcaaacg atcaaagcac gccggccgag ctgagcagag ctcgcagcgg
     1201 ctcgagctca cgctcgtagt ggcgccagcg ctcgagcccg gagcgataga tcggttgacg
     1261 aacttgcgcc gagctcgccg tcgcgacggg cgcggcattg ttctcgaact cgaggcagcg
     1321 cgagtcccaa ggcagcccgc aaaactcgac gagtcggcgc gcttcgcgct ccgtgcgatc
     1381 gacgacgtcc tcgtagcgga cctcgagata gttgtcggcc agcgattcgc gccaaagcgc
     1441 cgccagcgcg tggaacgctg cgtagtagcg cccggtgtcg agcaagtcgt aagcgtagtt
     1501 gtaataagag aagctcgtcg cgaacagctg ccggtagttg ctcaagcagg tgtcgagcgg
     1561 attacgtcgc aagcagacga tcttcgcgtt cggcagacag cgccggatca gcggcgcgta
     1621 gaagaagttc aacggcatct tgtcgatgaa ccgctgcttg tgccccgtgc gcggccgggt
     1681 gctctcgaca tacgcctcgc cgacggccgc aaagtcgcag cgcgcgcttg cgtccagtgt
     1741 ctccgcatcg aggacgcggt tcgacgccgt tgccgtcgcg cgcttcagca cgaggccgaa
     1801 gttcgtgagc tcgcccgcgg agaatacctt ggggtggctc gacaggatgc gctcgacgag
     1861 cgtcgtgccc gtgcgcggca tgccgacgat gaaaatcggc tcgtgcgagc catggccgtg
     1921 gccgtccccg gcgcgagcgg cgagcgcggc cggcaagcgc gcggcggccg cgaacaacga
     1981 gccgtccgcg gcgaagccat agccgagcgc cgcagccttg cggcgcttgc cgcgctcgag
     2041 caaacggaag gcggcgtcgt accgaccttc gtcctcgtgt tgcttcgcga gggcatgaca
     2101 gaggtgcagc tcggcgtcgg catcgagccc cggttgctcg agcgcgcgct cgagtgtcgc
     2161 gacgtcggca ggcgtcagcg gcgcgcgtgc gacttgagcc agcgacgacc acgcgcggtg
     2221 ataagtcggg tcgcggcgca gcgcttcacg gtactcgagc tccgcgtcct gcagcgcgcc
     2281 gctgaactgc agcgatgcgc cgaggttgta atgataagga gccttcgtgg aatcgcgcgc
     2341 aacggcgcgg cgaaacggct cgacggcttc cgtgtgcgcg ccggcgcgcg tcatgacgac
     2401 gccgatcgtg tcgagcgtca acgcgtcgtg cgggtcgagc tcgagcgctc gctgcgccgc
     2461 ttcgaaccct tcgcgcggcc gctgcagcga caataggcag cgcccgcgct gcgcccagta
     2521 ttccggcttc gccggcgcta gcgccagcgc gcggtcgatg atctcgacgg ctttgccgaa
     2581 gttgcggtgc tcggctgcga cgacgcccag caagaacaat gcgtccgcgt gttccggctc
     2641 cgcagtcagg atcttgaggc agagctcgtg ggcgaggcgg tagtcgcgcc ggttcagcgc
     2701 gtgctccgcc tctttatgcc actcagttac cacgccgtgc ggagggctcc atgttccaag
     2761 tgaagcggaa gccgatctcg cgcggccgca ccatctgttc cgcgtagctg cgcacgcgga
     2821 ccgggttgcc gttctcgtcg gcgaccgttt gaacaaacga gcggcgtgag cgcacgcccg
     2881 tgacggc
//
LOCUS       DATLEE010000334        11385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5599064, whole genome shotgun sequence.
ACCESSION   DATLEE010000334 DATLEE010000000
VERSION     DATLEE010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11385
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5599064"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..864)
                     /locus_tag="VM692_07130"
     CDS             complement(181..864)
                     /locus_tag="VM692_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ51977.1"
                     /translation="MSTRDSLLRAETLTKKVSSPEGELTILDSVTIDVPRGHTAAIVG
                     PSGAGKSTLLALLAGLDEPTSGKVWLNGAELTALDEDGRASLRNREVGFVFQSFHLLP
                     SLTAIENVLLPLELASTPNAATRARQALVDVGLEHRLRHYPKQLSGGEKQRVAIARAF
                     VTQPSLLFADESTGNLDNKTGAKILDLLFELNKAAGTTLVLVTHDLDAAGRCQHTIEL
                     AGGRILKAT"
     gene            857..1492
                     /locus_tag="VM692_07135"
     CDS             857..1492
                     /locus_tag="VM692_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HUQ51978.1"
                     /translation="MLIVKRLIVLLALSIICLPAAYGAGQTIVVVGDSLSSGYGIGAG
                     QSWVAMLEDRLQSEGYGYQVVNASIAGDTSAGGLARLPRLLSTHDPALVVIELGGNDG
                     LRGAPVATLRDNLTKMIELSQQSGAEVVLAGIQIPPNYGTTYTQALAKVYPELAERFD
                     AALIDFILADVALNRELMQPDGIHPNAAGQKIVFENVWRVLGPLLTPHDAG"
     gene            1482..3200
                     /locus_tag="VM692_07140"
     CDS             1482..3200
                     /locus_tag="VM692_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUQ51979.1"
                     /translation="MPAESLRATPAKPWLAAYSPGVPAEIDADRYTSLVALLEASFAR
                     FAAQPAFTNLDTTLTFADVERLSRAFAAYLQALPGMQAGDRVAIMIPNTLQSPVALFG
                     ILRAGMVAVNVNPMYTVPELEHQLADSGAKAVVVLENFAHTVAVALPRTAVKHVVVTQ
                     LGDHCALLKRLVVNFVVKRVKKLVRPWAIPGAVAYRDALARGAAASYTAPPVTGRDLA
                     FLQYTGGTTGRAKGAMLTQRNMVANTLQAAAWAGPFYAPDAGVVLTPLPLYHVYSLTA
                     NLLCFTELGAENVLITDPRDVKGFVELLRKTRFAFMTAVNTLFNALLHAPGFDSVDFS
                     TLRVCMGGGMAVQREVALRWQQVTGVAIAQGYGLTEASPIVTANPLHLKEFNGSVGLP
                     FPSTDVAVFDDEGRELGVGEVGEICARGPQVMAGYWQRPDETAQVLFGDGWLRTGDVG
                     RLDAQGYLYIEDRKKDVIVVSGFKVYPNEIEDVAVRQPGVREAAAIGVRDAQSGEVVK
                     LFIVRKDPMLTTAAVLAHARQNLTGYKIPRHVEFVDDLPKSNVGKVLRRALKERESQP
                     TASPPK"
     gene            complement(3173..4441)
                     /locus_tag="VM692_07145"
     CDS             complement(3173..4441)
                     /locus_tag="VM692_07145"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HUQ51980.1"
                     /translation="MNGPPAEPPAPDPGLARRLRRMRALATALLAAMAAIYAITTWFV
                     ARNPYLEALRSFAEAAVVGGLADWFAVTALFRRPLGLPIPHTAIVPARKNQIGRALAR
                     FIRDHFLVREAVARRLSRTDLAARLGAWLEDSDNSARVSRDVGGALAWALREDGSGGE
                     LRGALGLTLRSAFDEVPVNRAVATVLEVLTTGERANLIIDQLVQFGRTELANNRVMLR
                     VRIHEQSPWWLPKFVDQEIFDKLVGGLEELLDAIAADAAHPARVEIKARLDSLQQALG
                     ADPALAAKSRALKDELVAHPAVRNYAFELWQRLRAELEQSLTDAESPLTLGLRREIGA
                     LGARLRADSVLTAELDDWLKQVLLHVVDNYRDPLSEIVSETIESWDAQATSRRIELNI
                     GTDLQFIRVNGTIVGGLVGLALYLGGLAVG"
     gene            complement(4438..5265)
                     /gene="pyrF"
                     /locus_tag="VM692_07150"
     CDS             complement(4438..5265)
                     /gene="pyrF"
                     /locus_tag="VM692_07150"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HUQ51981.1"
                     /translation="MATAVTRPERRIANRERLIFALDVPDLDGARELVTRLGESVVFY
                     KIGLELATSRHYFELLDWLVARDKKVFTDLKLYDIPATVTAAVRQLSRSGASFLTVHG
                     DRAIMEAAAAAKGAQLKILVVTVLTSIAPQDLAEMGIGLSVEELVLLRARQAVAAGCD
                     GVIASGLEAEPLRKALGPQPLIVTPGIRPADGARAHAGRDAGGPATQARAGMPAGEQR
                     REQLPGLVADDQRRVVTPTIAFRSGADHIVVGRPIRDAADPYAAATAIQQEIAGVFG"
     gene            complement(5284..6438)
                     /locus_tag="VM692_07155"
     CDS             complement(5284..6438)
                     /locus_tag="VM692_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HUQ51982.1"
                     /translation="MTESRNELAPYAALAARSRGRQHAEPPPALRTEFQRDRDRIIHS
                     TAFRRLVYKTQVFVNYEGDLYRTRLTHSLEVAQIARTAARALRLNEDLAEAISLAHDL
                     GHTPFGHAGQDALNECMRDFGGFEHNLQSLRVVDHLEDRYASFRGLNLTFETREGILK
                     HCSLKHARELGEIGRRFLDRTQAGLEAQLANIADEIAYNNHDVDDGLRSGLVTLEQLA
                     ALPFFAEHLQRIARQYPQIKPQIKGSEAVRDMIGELVADLVRSSAASIAAAAPGNIDD
                     VRALPHGLIEFSAPLREAQLALKRFLRDALYTHPRVRQMTQQAHETVAYLFAELSADY
                     DRMPEEHAALAKERARSNDAAGAARVVADYIAGMTDRFALQTRDALRAKQ"
     gene            complement(6435..7505)
                     /gene="aroB"
                     /locus_tag="VM692_07160"
     CDS             complement(6435..7505)
                     /gene="aroB"
                     /locus_tag="VM692_07160"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HUQ51983.1"
                     /translation="MATIVVALGDRSYPIHIGPSLLDKADLYALTAKQVLIVTNEVVA
                     PLYLERVRTALRGREIETVVLPDGERHKTLATFTSVIDRLIDGRFHRDCCLVALGGGV
                     VGDLTGYVAASYQRGVDFVQVPTTLLAQVDSSVGGKTAVNHPRAKNMIGAFHQPIAVL
                     ADTDTLTSLPPRELAAGLAEVIKYGIIVDATFFAWLEAHVDDLRRLDPAALTHAIRRS
                     CEIKAVIVAEDEREQGRRALLNLGHTFGHALEAVGHYERWLHGEAVAIGMALAARTSA
                     ALGWLKVSDCERIESLLARAGLPTTASGVDADELLELMRGDKKADRAGLKLILIRSIG
                     ESVVARSPAEAELRAVLTERLK"
     gene            complement(7519..8058)
                     /locus_tag="VM692_07165"
     CDS             complement(7519..8058)
                     /locus_tag="VM692_07165"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HUQ51984.1"
                     /translation="MFGQRNVYLIGPMGSGKTAVGRRLATLLSKHFFDSDAEIEKRTG
                     VDIRYIFEKEGETRFREREREVIADLTALDGVVVATGGGVILDAKNRARLTETGTVVY
                     LETNVETLVRRTKASKTRPLLLNDDPRSVLERMMLIRRPLYEGTADLRIETTGRQVRA
                     VAADIQQRLAQRVAKPAST"
     gene            complement(8133..10298)
                     /locus_tag="VM692_07170"
     CDS             complement(8133..10298)
                     /locus_tag="VM692_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HUQ51985.1"
                     /translation="MNMSALVRRENSKSWLAQLASLGWLLALGLVSPAAQAQDGLRLE
                     AIDVQPLSGQQVELRLRLSGTAPEPMTFTIDDPARISLDLPNTTLALDSRRQDANVGP
                     LTTVLAAEANGRTRVVMNLSAMVPYQTRVEGNNVVVVLGQAPGEAAGPSFAAQPSQSA
                     TPRVAAAAGDRSIRNIDFRRGSDGSGQIVVDLNDPRTTVDVREEGGRIVVDFQNTTLP
                     QELMRRLDVTDFATPVFTVDALRANRDARLIVTAAANYDQVAYQSDNQFTLELKPPVQ
                     QAAAAPGVFDAEREYTGERLTLNFQDLETRAVLQLIADFSGLNIVVSDTVQGSVTLRL
                     QNVPWDQALDIVMTTKGLDMRRNGNTMIVMPAEEIAAREQAELEALQALQTLEPLRSE
                     FIQVNYAKAAELASLIEGEGENSLLTDRGSVAIDERTNTLLVNDTPTSLANIRRLVTT
                     LDIPVQQVLIESRVVIVNDDYSRELGVRFGTTSVNDNSGNGLLGLTGTAGGASTLVGT
                     PGLGGAGGSILPVTAPSAGSRYNVNMPVLGPAGSIALAILGNDYLVDLELSALQAEGD
                     GRVVSTPRIVATNQREARILQGTEIPYQEASSSGATTTSFKEAVLSLTVVPQVTPDNH
                     IIMDLTVTKDSVGEVFNNVPAIDKRELQTQVVVADGETVVLGGIYETELVNAERKVPG
                     LGNIPGLGHLFRSRTTENNNAELLIFVTPKILREGNRVN"
     gene            complement(10327..10857)
                     /locus_tag="VM692_07175"
     CDS             complement(10327..10857)
                     /locus_tag="VM692_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HUQ51986.1"
                     /translation="MVTRSVLSTRWVTLLVATVGLSACGGANDDLKTYIDEVKARPGG
                     RIEPLPQVQPAPTFVYEAGTRRSPFTPDAPQRRVSNDPNSIEGPDQNRPREFLEQFPL
                     DTLRMVGTLADRRASFGLVQTTDGLVHRVAVGNHMGQNYGRIVSISDSEIQLVEIISD
                     GLGGYLERPAAIALAD"
     gene            complement(10857..>11385)
                     /locus_tag="VM692_07180"
     CDS             complement(10857..>11385)
                     /locus_tag="VM692_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HUQ51987.1"
                     /translation="VALVFAAVVAAGTYWFVIQEEVPRLERAQAEERDLRSQFEERQR
                     KAANFDAYRAQLAEIERDFGAMLRQLPGQTEVPSLLVDISQTGLGAGLEEELFQPGVE
                     IHKDFYAELPIKLRYSGSYHELGNFVSGIAALPRIVTLHDVTIRPRNDSSPDELILDV
                     TAKTYRYLDEEATEG"
BASE COUNT         1838 a         3698 c         3859 g         1990 t
ORIGIN      
        1 tcgagcttct tcaggcaaca gcgcccgcag ccgtcgcaga gcgactccca ctccgcgtcc
       61 gtcagctccg caagcgtctt acgctgccaa aacggctgca attcacgcct tgtttcgaat
      121 tcgtccacgc gctcattgta agccgtcgac tcgcggagcg acggctcgcg gcctgaatcg
      181 tcaggtggct ttgaggattc gcccgcccgc gagctcgatc gtgtgctgac agcggccggc
      241 cgcgtcgagg tcgtgcgtga cgagcacgag cgtcgtgccc gcggccttgt tcagctcgaa
      301 tagcagatcg aggatcttcg caccggtctt gttgtctaga ttgccggtcg actcgtcggc
      361 gaatagcagc gaaggctgcg tcacgaacgc ccgcgcgatg gcgacgcgtt gcttctcgcc
      421 gccggacagc tgcttcggat aatgacgcag ccggtgctcg aggccgacgt cgacgagcgc
      481 ttgccgcgcc cgcgtcgcgg cgttcggcgt cgaagccagc tcgagcggta acaatacgtt
      541 ctcgatcgca gtcaacgaag gtaacaagtg gaacgattga aaaacgaagc caacctcgcg
      601 attgcgcagg ctcgcgcgcc cgtcctcgtc gagcgccgtg agctcggcgc cattgagcca
      661 aaccttgccc gacgtgggct cgtcgagccc cgccagcagc gccagcagcg tcgatttgcc
      721 ggcaccggaa ggccccacga tcgcggccgt gtggccgcgg ggcacgtcga tcgtgacgga
      781 atcgaggatg gtcagctcac cctccgggct gctaaccttt ttcgtcagag tctcggctcg
      841 taacagggaa tcgcgggtgc tcattgtcaa acgtctgatc gtcttgctcg cgctgagcat
      901 tatctgccta ccggcagcgt acggcgccgg gcaaaccatc gtcgtggtgg gcgactcctt
      961 gagtagcggc tatggaatag gcgctgggca atcttgggtc gcgatgctcg aggatcgcct
     1021 gcagtcggaa ggttacggtt atcaagtagt taatgccagc atcgccggcg acacgtcggc
     1081 gggcggtctc gcacggctgc cgcgactgct ctcgacgcac gacccggcgc tcgtggtcat
     1141 cgagctcggc ggcaatgacg gcttgcgcgg cgcgccggtc gcgaccttgc gtgacaactt
     1201 gacgaagatg atcgagctca gtcaacaaag cggcgccgag gtcgtgctcg ccggcatcca
     1261 gattccgccg aactacggaa ccacttacac gcaggcgctg gccaaggtgt acccggagct
     1321 cgccgagcgc ttcgatgcag ctttgatcga cttcatcctg gccgacgtcg cgttgaaccg
     1381 cgagctcatg caacccgacg gtatccatcc gaacgccgcg gggcagaaga tcgtcttcga
     1441 gaacgtctgg cgcgtgctcg gcccattgct gacgccccac gatgccggct gaatccctcc
     1501 gcgcgacgcc ggccaagccg tggcttgcgg cgtactcgcc gggcgtgccg gccgagatcg
     1561 atgccgaccg ctatacctcg ctcgtggcgc tgctcgaagc gagcttcgcg cggttcgcgg
     1621 cgcagccggc attcacgaat ctcgacacga cgctgacgtt cgcggacgtc gagcgcctga
     1681 gccgcgcctt tgccgcgtat ctgcaagcgc tgcctgggat gcaagccggg gatcgcgtcg
     1741 cgatcatgat cccgaacacg ctgcagtcgc cggtcgcctt gttcggcatc ctgcgggccg
     1801 gcatggtcgc cgtgaacgtc aacccgatgt acacggtgcc ggagctcgag caccaactcg
     1861 cggacagcgg cgcgaaggcc gtcgtcgtgc tcgagaattt cgcgcatacg gtcgccgtgg
     1921 cgctgccgcg cacggccgtg aaacacgtcg tcgttacgca gctcggcgat cattgcgcgc
     1981 tgctgaaacg cctcgtcgtc aacttcgtcg ttaaacgcgt caagaagctc gtacggccgt
     2041 gggcgatccc gggcgccgtc gcttaccgcg atgcgttggc tcgcggcgcg gcagcgtcgt
     2101 acacggcacc gcccgtcacg ggccgcgacc tcgcgttcct gcagtacacg ggcgggacga
     2161 cgggccgcgc caaaggcgcg atgctgacgc agcgcaatat ggtcgcgaac acgctgcagg
     2221 ccgcggcctg ggcggggccg ttctacgcgc ccgatgcggg cgtcgtgctg acgccgctgc
     2281 cgctctatca cgtctactcg ctgacggcga acttgctgtg cttcacggag ctcggtgcgg
     2341 agaacgtgct gatcaccgat ccgcgcgacg tcaaagggtt cgtagagctg ttgcggaaga
     2401 cgcgcttcgc gttcatgacg gccgtcaaca cgctgttcaa cgcgctgctg cacgcgccgg
     2461 gcttcgactc ggtcgacttc tcgactctgc gcgtctgcat gggcggcggc atggccgtgc
     2521 agcgcgaggt cgcgctgcgt tggcagcaag tgaccggcgt cgccatcgcg cagggctacg
     2581 ggctcacgga agcgtcgccg atcgtcaccg ccaatccatt gcatctcaag gagttcaacg
     2641 gctccgtggg gctgccgttc ccgtcgacgg acgtcgcggt cttcgacgac gagggtcgag
     2701 agctcggtgt cggcgaggtc ggcgagatct gcgcgcgcgg cccgcaagtc atggccggtt
     2761 attggcagcg gcccgacgag acggcgcaag tgctgttcgg cgacggttgg ctacgcacgg
     2821 gcgacgtcgg gcggctcgac gcgcaaggct atctctacat cgaggaccgc aagaaagacg
     2881 tcatcgtcgt ctcgggcttc aaggtgtatc cgaacgagat cgaggacgtc gcggtgcgtc
     2941 agcccggcgt tcgtgaagcg gcggcaatcg gcgttcgcga cgcgcagtcg ggcgaggtcg
     3001 taaagctgtt catcgtccgc aaggatccaa tgctgacgac cgcggccgtg ctcgcgcacg
     3061 cgcgccagaa cctgacgggc tacaagatcc cgcggcatgt cgagttcgtc gacgacctgc
     3121 cgaagagcaa cgtcggcaag gtgctgcggc gcgcgctcaa ggaacgcgag tctcagccga
     3181 ccgcgagccc gcccaagtag agcgcgagac cgacgagccc gccgacgatc gtgccgttga
     3241 cgcggatgaa ttgcaggtcc gtgccgatat tgagctcgat gcgccgcgag gtcgcctgcg
     3301 cgtcccagct ctcgatcgtc tcggacacga tctcgctcaa cgggtcgcga tagttgtcga
     3361 cgacgtgcag cagcacttgc ttcagccagt cgtcgagctc ggcagtgagc accgagtccg
     3421 cgcgcaggcg cgcgccgagc gcgccgatct cgcggcgtag gccgagcgtc agcggcgact
     3481 ctgcgtcggt cagcgactgc tcgagctctg cgcggaggcg ctgccacagc tcgaacgcat
     3541 agttgcgcac ggccggatgc gcgacgagct cgtctttcag cgcgcggctc ttggcggcca
     3601 gtgccgggtc cgcgccgagc gcttgttgca gcgagtcgag ccgcgccttg atctcgacgc
     3661 gcgccggatg cgcggcgtcg gcggcgatcg cgtcgagcag ctcctcgagg ccgccgacga
     3721 gcttgtcgaa aatctcctgg tcgacgaact tcggtagcca ccacgggctc tgctcgtgaa
     3781 ttcgcacgcg cagcatcacg cgattgttcg cgagctccgt gcggccgaac tggacgagct
     3841 ggtcgatgat cagattggcg cgctcgcccg tcgtcaggac ctcgagcacc gtggcgacgg
     3901 cgcgattgac aggcacttcg tcgaacgccg atcgcagggt cagacccaac gctccacgca
     3961 gctcgccgcc gctgccatcc tcgcgcaacg cccaggcaag cgcgccgccg acgtcgcggc
     4021 ttacgcgtgc cgagttgtcg ctatcttcga gccaggcgcc taggcgcgcg gcgagatccg
     4081 tgcggctcag ccgtcgtgcc acggcttcac gcacgagaaa gtggtcgcgg atgaagcgcg
     4141 ccaaggcgcg gccgatctgg tttttgcgcg caggcactat cgccgtgtgt gggataggca
     4201 gaccgagcgg ccggcgaaac agcgccgtga cggcgaacca gtccgcgagc ccgccgacga
     4261 cggcggcctc ggcgaaggag cgcagcgctt cgaggtaggg attgcgcgcg acgaaccagg
     4321 tcgtgatggc gtagatcgca gccatggccg ccagcagcgc ggttgcgagc gcgcgcatgc
     4381 ggcgcaagcg gcgtgcaagc cctggatccg gcgctggcgg ctccgccggc gggccgttca
     4441 tccaaagacg ccggctatct cttgctgaat cgccgtggcc gccgcatacg ggtcagccgc
     4501 gtctcgaatc ggcctgccga cgacgatgtg gtcggcgccc gagcggaacg cgatcgtcgg
     4561 cgtcacgacg cggcgctgat cgtcggcaac taatcccggc aactgctccc tgcgttgctc
     4621 ccccgcgggc atcccggccc gcgcctgagt tgctggaccg cccgcatccc tgcccgcgtg
     4681 cgcacgcgca ccgtcggccg ggcggatgcc aggcgtcacg atcaatggct gaggacccaa
     4741 tgctttgcgc agcggctcgg cctcgaggcc cgacgcgatg acgccgtcgc agccggcggc
     4801 cacggcttgc cgcgcacgca gcaacacgag ctcctcgacc gaaaggccga tgcccatttc
     4861 ggcgaggtct tgcggcgcga tgctcgtcag caccgtgacg acgaggatct tcagttgcgc
     4921 gcccttggct gccgccgcgg cctccatgat ggcccggtcg ccgtgcaccg tgaggaacga
     4981 ggctccggac cggctcagtt ggcggacagc cgccgtcacg gtcgcgggaa tgtcgtaaag
     5041 cttcaagtcg gtgaaaacct tcttgtcgcg cgccacgagc cagtcgagca gctcgaagta
     5101 gtggcgcgac gtcgcgagtt cgaggccgat cttgtagaac accacgctct cgccgagcct
     5161 cgtgacgagt tcccgcgcgc catcgagatc cggcacgtcg agcgcaaaga tcagccgctc
     5221 gcggttcgcg atgcggcgct cgggacgtgt cactgcggtt gccacggctg aattctagca
     5281 gcgctactgt ttggcgcgca gggcgtcgcg agtctgtaga gcgaagcgat ccgtcatgcc
     5341 ggcgatatag tcggcgacca cgcgcgcggc gcccgccgcg tcgttactgc gcgcgcgctc
     5401 cttggcgaga gccgcgtgct cctcgggcat tcggtcgtag tcggccgata gttctgcgaa
     5461 gaggtatgcc acggtctcgt gcgcttgctg cgtcatctgc cgcacgcgcg gatgcgtgta
     5521 caacgcgtcg cgcaggaaac gcttcagcgc gagctgagcc tcacgcaacg gcgcgctgaa
     5581 ttcgatgagc ccgtgcggaa gcgcgcgcac gtcgtcgatg ttgcccggcg ccgctgcggc
     5641 aatcgaagcc gcgctgctgc gaacgagatc cgcgacgagc tcgccgatca tgtcgcggac
     5701 cgcttcgctg cctttgatct gcggcttgat ttgcgggtac tgacgcgcga tacgctgcaa
     5761 gtgctcggca aagaacggca gcgccgcgag ttgctccaat gtgacgagcc cggagcgaag
     5821 cccgtcgtcg acgtcgtggt tgttgtaggc gatctcgtcg gcgatgttcg cgagctgcgc
     5881 ctcgagcccc gcttgcgttc gatcgaggaa gcgccggccg atctcgccga gctcgcgcgc
     5941 atgcttcagc gagcagtgct tgagaattcc ttcgcgcgtc tcgaacgtca ggttcaggcc
     6001 gcggaagctc gcgtagcgat cctcgaggtg atcgacgact cgcagcgact gcaagttgtg
     6061 ctcgaagccg ccgaagtcgc gcatgcattc gttgagcgca tcctggccgg cgtgcccgaa
     6121 cggggtgtgg ccgaggtcgt gcgcgaggct gatggcttcc gccaggtcct cgttcaagcg
     6181 caacgcgcgc gcggcagtgc gggcaatctg cgcgacctcg agcgaatgcg tgagccgcgt
     6241 gcgatacagg tcgccctcgt agttgacgaa tacctgggtc ttgtagacga ggcggcgaaa
     6301 agccgtggaa tgaatgatgc gatcgcgatc gcgttggaac tccgtccgca gcgccggcgg
     6361 cggctcggca tgctgtcggc cgcgcgagcg tgcggccaag gcggcgtacg gcgcgagctc
     6421 gttgcgcgac tccgtcactt caggcgctcg gtcagcacgg cgcgcagctc cgcctcggcc
     6481 ggcgagcgcg ccacgaccga ctcgccgatg ctgcgaatga gaatgagctt gaggcctgcg
     6541 cgatcggctt tcttgtcgcc gcgcatgagc tcgagcagct cgtcggcgtc gacgccggac
     6601 gccgtcgtcg gcagccccgc gcgcgcgagg aggctctcga tgcgttcgca gtcgctgacc
     6661 ttgagccacc cgagcgcggc cgacgttctg gccgcgagcg ccatgccgat ggcgacggct
     6721 tcgccgtgca gccagcgctc gtagtggcca acggcctcga gagcgtggcc gaacgtgtgg
     6781 cccaagttca gcagcgcgcg acggccctgc tcgcgctcgt cctcggcgac gatgacagcc
     6841 ttgatctcgc agctgcggcg gatggcgtgc gtcagcgcag caggatcgag gcgccgcaag
     6901 tcgtcgacgt gcgcctcgag ccacgcgaaa aacgtcgcat cgacgatgat gccgtacttg
     6961 atgacctcgg cgaggcctgc ggcgagctcg cgcggcggca gcgacgtcag cgtgtcggta
     7021 tcggccagga ccgcgatcgg ttgatggaac gcgccgatca tgttcttcgc gcgcggatga
     7081 ttgacggccg tcttgccgcc gaccgacgag tcgacctggg caagcagagt cgttgggact
     7141 tgcacgaagt cgacgccgcg ctggtagctc gccgcgacgt agcccgtcaa atcgccgaca
     7201 actccgccgc cgagagcgac gaggcagcag tcgcgatgga agcggccgtc gatgaggcgg
     7261 tcgatgacgc tcgtgaacgt cgccagcgtc ttgtggcgct cgccgtccgg cagaaccacg
     7321 gtctcgatct cgcggccgcg aagagcggtc cgcacgcgct cgaggtagag cggcgcgacg
     7381 acctcgttgg tgacgatgag cacctgcttg gccgtgagag cgtagaggtc cgctttgtcc
     7441 agcagcgatg gcccgatgtg gatcgggtag ctgcggtcac cgagtgcgac gacgatggtt
     7501 gccacgcgcc gcaggttctc aagtagacgc gggctttgca acgcgctgcg cgagccgttg
     7561 ctggatgtcc gcggcgacgg cgcgcacttg gcgccccgtg gtttcgatgc gcaagtcggc
     7621 agtgccctcg tatagcggcc ggcggatcaa catcatgcgc tcgagcacgc tgcgcggatc
     7681 gtcgttcagc aacaacgggc gcgtcttcga cgctttcgtg cgacgaacga gcgtctcgac
     7741 gttcgtctcg agatagacga cggtgccggt ttcggtcagg cgcgcgcggt tcttggcatc
     7801 gaggatcacg ccgccgcccg tcgcaacgac gacgccgtcg agcgccgtga ggtccgcgat
     7861 gacttcgcgc tcgcgctcgc gaaaccgcgt ctcgccttct ttctcgaaaa tgtagcggat
     7921 gtcgacgccg gtgcgcttct cgatttcggc gtcgctgtcg aagaagtgct tgctcagcag
     7981 cgtggcgagt cgccggccga cggcggtctt gccggagccc atggggccga tcaagtagac
     8041 gttacgctgg ccgaacatgg ctgcgtagga tactgaacgc aagacggccg ccccaagggg
     8101 cggccgtccg caaacaaact cgctcggtgc gcttagttga cgcgattgcc ttcgcgcagg
     8161 atcttcggtg tcacgaagat cagcaactcg gcattgttgt tctcggttgt tcggctgcgg
     8221 aacaggtgcc cgaggcccgg gatgttgcct aggcctggta cttttctctc tgcgtttacg
     8281 agctcggtct cgtagatgcc gccgagcacg acggtctcgc cgtcggcgac cacgacttgc
     8341 gtctgcagct cacgcttgtc gatcgcgggg acgttgttga agacttcgcc gacggagtcc
     8401 ttcgtgacgg tcagatccat gatgatgtgg ttgtccggcg tgacctgtgg gacgaccgtc
     8461 aagctcagca ccgcctcctt gaacgacgtc gtcgtcgcac cgctcgacga cgcttcctga
     8521 tacgggattt ccgtaccctg taggatgcgc gcttcgcgct ggttcgtagc gacgatgcgc
     8581 ggcgtcgaca cgacgcggcc gtcgccttcg gcttgcaacg ccgagagctc gagatcgacg
     8641 agatagtcgt tgccgaggat cgcgagggcg atcgagccgg cggggcccag gaccggcatg
     8701 ttcacgttgt agcgcgagcc ggccgatgga gccgtgaccg gcaggataga gccgcccgca
     8761 ccgcccaggc cgggggtgcc gaccagggtg ctggcgccgc cggccgtgcc cgtgaggccg
     8821 agcaggccgt tgccggagtt gtcgttcacc gacgtcgtgc cgaagcgcac accgagctcg
     8881 cggctgtaat cgtcgttgac gatgacgacg cggctttcaa tgagcacctg ctgcacgggg
     8941 atgtcgagcg tggtcacgag gcggcggatg tttgccaggc tcgtcggcgt gtcgttgacg
     9001 agcagcgtgt tggtgcgctc gtcgatcgcg acgctgccgc gatcggtcaa cagcgagttt
     9061 tcgccctcac cctcgatgag gctcgcgagc tccgccgctt ttgcgtagtt gacctgaatg
     9121 aattcggagc gcagcggctc cagcgtctgc aacgcctgta gagcctcgag ctcggcctgc
     9181 tcgcgtgccg cgatttcttc ggccggcatc acgatcatcg tgttgccgtt gcggcgcatg
     9241 tcgaggccct tcgtcgtcat gacgatgtcg agcgcctgat cccacgggac gttttgcagg
     9301 cgcaaggtca cactgccttg caccgtgtcg ctgaccacga tgttgaggcc gctgaagtcg
     9361 gcgatcagtt ggaggacggc gcgcgtctcg aggtcttgga agttcaacgt caggcgctcg
     9421 cccgtgtact cgcgttcggc gtcgaacacc ccgggcgccg cagcggcctg ctgaaccggc
     9481 ggcttgagct cgagcgtgaa ctgattgtcc gactgatacg cgacctggtc gtaattcgcg
     9541 gctgcggtga cgatgaggcg cgcatcgcga ttcgcgcgca aagcatcgac ggtgaacacc
     9601 ggcgtggcga agtccgtgac gtcgagccgg cgcatgagct cttgcggcag cgtcgtgttc
     9661 tggaagtcca cgacgattcg gccgccttcc tcacgaacgt ctaccgtcgt gcgcggatcg
     9721 ttgaggtcga cgacgatctg gcccgagccg tcggagccgc ggcggaagtc gatgttgcgg
     9781 atcgagcggt cgccggcggc ggccgcaacg cgtggtgtag ccgactggct cggctgcgcg
     9841 gcgaacgacg ggccggcggc ctcgccgggc gcctggccga gcaccacaac gacgttgtta
     9901 ccttcgacgc gcgtctgata gggaaccatc gcgctcaagt tcatgacgac gcgggtacgg
     9961 ccgttcgctt cagccgcgag gacggtagtc aacggaccga cgttggcgtc ttgacgccgc
    10021 gagtcgagcg cgagcgtcgt gttcggcaag tccagcgaga ttcgcgccgg gtcgtcgatc
    10081 gtgaacgtca tcggctccgg cgccgtgccg ctcaagcgca gccgtagctc gacctgttgt
    10141 ccggacaagg gctggacgtc gatggcttcc aagcgcagcc catcctgcgc ttgcgctgca
    10201 ggggacacga ggccgagcgc gagaagccag ccgaggctcg cgagctgggc gagccacgac
    10261 ttcgagttct cgcgacggac gagcgctgac atattcatac ggttaactcc ctaaatcccc
    10321 tgcgcttcaa tcggctaagg caatcgcagc aggtcgttcc aagtacccgc cgagaccgtc
    10381 ggagatgatc tccaccaatt gaatctctga atccgagatg gaaacgatgc ggccgtagtt
    10441 ctggcccatg tggttgccca cggcgacgcg gtgcacgagg ccgtccgtcg tctggacgag
    10501 accgaagctg gcgcgccggt cggcgagcgt gccgaccatg cgcagcgtgt cgagcggaaa
    10561 ttgctcgaga aactcgcgcg gccgattttg atccgggcct tcgatggagt tcggatcgtt
    10621 gctgacgcgc cgctgcggcg cgtcgggcgt gaacggcgac cgccgagtgc ccgcctcgta
    10681 aacgaacgtc ggcgcaggtt gcacctgcgg caacggctcg atgcgtccgc cgggccgggc
    10741 tttgacctcg tcgatatagg tcttcaaatc gtcgttggcg ccgccgcacg cgctcaggcc
    10801 aacggtggcg accaaaaggg tcacccagcg cgtcgagagt acgctacgcg tcaccatcat
    10861 ccctccgtcg cttcctcgtc gaggtagcga taagtcttcg ccgtcacgtc gagaatcagc
    10921 tcatcgggcg atgagtcgtt tcgcgggcga atcgtgacgt catgtagcgt cacaatgcgc
    10981 ggcagtgcag cgatgccgct cacaaaattg ccgagctcgt ggtagctgcc cgagtagcgc
    11041 agcttgatcg gcagctccgc gtagaagtcc ttgtgaatct cgacgcccgg ctggaacagc
    11101 tcctcttcga ggcccgcgcc caagcccgtc tgcgagatgt cgaccaacag gctcggcacc
    11161 tcggtctgac ctgggagctg acgcagcatg gcgccgaagt cgcgctcgat ctctgcgagc
    11221 tgcgcgcggt acgcgtcgaa gtttgcggcc ttgcgctgcc gttcctcgaa ttgcgagcgc
    11281 aaatcgcgct cttcggcctg cgcgcgttcg agtcgtggca cttcttcctg gatcacaaac
    11341 caataggttc ctgccgcgac gacggcggca aatacgaggg cgacc
//
LOCUS       DATLEE010000335         2256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_383486, whole genome shotgun sequence.
ACCESSION   DATLEE010000335 DATLEE010000000
VERSION     DATLEE010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383486"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1378
                     /locus_tag="VM692_07185"
     CDS             188..1378
                     /locus_tag="VM692_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HUQ51988.1"
                     /translation="MASREFKVGAFTLAGLTVVGFVVFMIGEERKLFESQLSYNAKFE
                     EVQGLRRGSPVRMGGVDIGSVTEVKYGAAVEDKQIHVKMSVVKDESRRIRQDSVAAIE
                     SKGLLGDKMIVITVGNQRLPQIPVGGEVPSKEGDDMAAMMGKLGSITVQVEKVVGNLE
                     RTTNSLADEKLHGDLKSSVESMNGILKSVDGGDGYVGKLIKSPEESQRLSHVMQNLET
                     ATAQLNRTTASINSILARVENGPGLAHEVLYGEESAKAVSQFGGAADELRLTLKGIRD
                     GNGIARSVIYGDEGSQQMMSNLNQMSGDLKQIVGDMKSGKGTLGALLVDPSVYEDLKI
                     VLGNVERNKALRALVRYSIRRDGAQPGVEVRDPAPNAVGAKKPDGAEKLSVEAGASTT
                     PPPQ"
     gene            1510..>2256
                     /locus_tag="VM692_07190"
     CDS             1510..>2256
                     /locus_tag="VM692_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51989.1"
                     /translation="MLQLPSRRFGLFSSLSLFALAALPISACSTANDGTGAPASCSGL
                     DTSVRAQATLRAYAEAVTSLRDRAAEVEGKFLSVCNAINGDLGLDTSKTTAEQACGVL
                     KQRIDAAAKAGVTVEAQIEFNCHADVSVQADCEASCQVEASCDVKAQCTGGEVVVECN
                     GMCSGQCDVSAPSVECHGSCQGDCSADVSAMCAGECEGTCSAPRFDGTCDAGCSANFS
                     GSCEGTCAGKCDGKDASGKCAGKCEGTCTGK"
BASE COUNT          452 a          677 c          756 g          371 t
ORIGIN      
        1 ccgacttcat cgaaggccgc gcccccgtca aagaatccgt cgagacgctt ttgagctcgt
       61 agccgcgtac aggctcggcg caatacgccg tacgttgggc cggaatgccg cgacattgcg
      121 tggtttgcgg cggggggtga aagtggcaag attccggttc cttatgtccg gctgggatcg
      181 ataagcgatg gcctcacgtg aattcaaggt gggagcgttc acgctcgcgg gcctcacggt
      241 cgtcggcttc gtcgtgttca tgatcggtga agagcgcaag ctcttcgagt cgcagctctc
      301 gtacaacgcc aagttcgaag aggtacaagg gctacgccgc ggctcgcccg tgcgcatggg
      361 cggcgtcgac atcggcagcg tgaccgaggt caagtatgga gccgcggtcg aagacaaaca
      421 gatccacgtc aagatgagcg tggtgaagga cgagtcgcgc cgcatccgtc aggacagtgt
      481 cgccgcgatc gagagcaaag gtctgctcgg cgacaagatg atcgtcatca ccgtcggcaa
      541 ccaacggctc ccgcagattc ccgtgggcgg tgaggtgccg tccaaagaag gcgacgacat
      601 ggccgccatg atgggcaagc tcggcagcat caccgtgcaa gtcgagaagg tcgtcggcaa
      661 tttggagcgg acgaccaatt cgttggctga cgagaagctg cacggcgacc tcaagagcag
      721 cgtcgagtcg atgaacggca tcctcaagtc ggtcgacggc ggcgacggct acgtcggcaa
      781 gctgatcaag agccccgaag agtcacagcg cctatcacac gtcatgcaga acctagagac
      841 tgccacggcg cagctcaatc gcacgacggc cagcatcaac tctatcttgg cgcgggtcga
      901 aaacggcccc ggcttggcgc acgaagtgtt gtacggcgaa gaatcagcca aggccgtgag
      961 ccaattcggc ggcgccgcgg acgagctgcg cctcaccctc aagggtatcc gcgatggcaa
     1021 cggcatcgcg cgcagcgtga tctacggcga cgaaggctcg cagcagatga tgagcaatct
     1081 gaaccaaatg agcggcgacc tgaagcagat cgtcggcgat atgaagtccg gcaagggcac
     1141 gctgggggcg ctgctcgtcg atccttcggt gtacgaagac ttgaaaatcg tgctcggcaa
     1201 cgtcgagcgc aacaaggccc tgcgcgctct cgtccgctac tccatccgtc gcgacggcgc
     1261 ccagccggga gtcgaagtgc gcgaccccgc gcccaacgcg gtaggcgcca agaagccgga
     1321 cggcgccgag aagctctcgg tcgaggccgg cgcgtcgacg acgcctcctc cgcaatagcg
     1381 ttcgttcgcg gcccgttgct ggggcgcaaa tggatctcac ggtgcgaaaa tgcgtcgccg
     1441 cgtccacgcg tgatccgcgc aaaaagggcg gttaagtggc gctggctcgg cttttgctga
     1501 gggggcgcca tgcttcaact cccttctcgc cgtttcggtc ttttttcatc actctcgctg
     1561 ttcgcgctgg cggctttgcc gatctcggct tgttcgaccg ccaatgacgg cacgggcgcc
     1621 cccgccagct gcagtggcct cgacaccagc gttagggccc aagcgacgtt gcgcgcgtac
     1681 gccgaggcgg tgacgtccct gcgcgaccga gcggcggagg tcgagggaaa attcttgagc
     1741 gtgtgcaacg ccatcaacgg cgatctcggc ctcgatacga gcaagaccac cgccgagcaa
     1801 gcttgcggcg tgttgaagca gcgcatcgac gcggccgcca aggccggggt gacggttgaa
     1861 gcccaaatcg agttcaactg tcacgccgat gtctcggttc aagcggactg cgaagccagc
     1921 tgccaggtcg aagccagctg cgatgtcaaa gctcaatgca cgggtggcga ggtggtggtc
     1981 gagtgtaacg gcatgtgcag cggtcaatgt gacgtcagcg cgcccagcgt cgagtgccac
     2041 ggctcttgtc agggggactg ctcggccgac gtcagcgcga tgtgcgcagg cgaatgtgaa
     2101 ggaacctgta gcgcgccgcg tttcgacggc acgtgcgacg ccggttgcag cgccaacttc
     2161 tccggcagct gcgaaggcac gtgcgcgggc aagtgtgacg gcaaggacgc gagcgggaaa
     2221 tgcgcaggaa agtgtgaagg cacctgcacc ggcaag
//
LOCUS       DATLEE010000336         3131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1130761, whole genome shotgun sequence.
ACCESSION   DATLEE010000336 DATLEE010000000
VERSION     DATLEE010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130761"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VM692_07195"
     CDS             <1..880
                     /locus_tag="VM692_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HUQ51990.1"
                     /translation="FRYTPPMSDDRLGSTLFLAALVHGVVILGVTFTAAPFGDGEALP
                     SLNVTLVLNTEREELAADTADFIANRDQRGAGEAARGLRPTSTLSADQPVTQVGDPLG
                     ADATDGTPREETPSAEQVVSRGASSEAIAALPQPTDDPALTRQRAAALIQQAAPQTTA
                     MELALRAELPDGDDDARTLIATPSTRRSDLAEYLDGWRRRVERIGTANYPTGLLGNID
                     AGRPTLEVVIGADGRLDDIVVRQSSGDKALDQAALKILRLAAPFAPLPDEIRKDYDVL
                     RFAYEWDFAGGAASRR"
     gene            935..1489
                     /locus_tag="VM692_07200"
     CDS             935..1489
                     /locus_tag="VM692_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HUQ51991.1"
                     /translation="MQSLQDHFLIAMPAMADPNFNETVTYICKHDTEGAFGIVINRPS
                     DLSLGEMLTQLAINLTDRRLADRPIMHGGPVEPERGFVLHRSDQAYEATLAAGSEIKL
                     TSSPDVLAALGRGTGPEPVLVALGYAGWGRGQLEIELGSNTWLTVPANPTIIFETPCE
                     QRWTAALGLPGVDIRRITHYAGHA"
     gene            1507..1938
                     /gene="ruvX"
                     /locus_tag="VM692_07205"
     CDS             1507..1938
                     /gene="ruvX"
                     /locus_tag="VM692_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HUQ51992.1"
                     /translation="MARASDHHLLVAFDFGLRRIGIATANRETGTASPLTTLRVERDL
                     PWVEIDRVIEDWQPAQLVVGVPEGEGAAAIARRARAFAAGLAERYGLPVATVDETLTS
                     VAAETELAEKRRSGYLRRRIGKGSVDRVAACLIAEQWMREQ"
     gene            1949..2824
                     /locus_tag="VM692_07210"
     CDS             1949..2824
                     /locus_tag="VM692_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HUQ51993.1"
                     /translation="MSYESNRGSVYLEDAPILAQRAYPGEQFVLRVQAPRAARRATPG
                     SFAHISCDPSVAMRRPLSIMRASPSEGWLEFLYKPKGHGLEMLGFRQPGEAVSVLAPI
                     GHGFTVDRARPRLLALGGGVGIPPMIFLAEQVRADRTLRPLVLMGSEVPFPFDLVESR
                     APVPGVKKPATHAVALLEQWGVPSRLASNACLPGAHRGYITDLARDALQAMTNSERTE
                     VQVFACGPTPMLKAVAKLARDFDLPCQVALEEYMACGIGGCAGCTVLLQTPDGPAMKR
                     VCVDGPVFDARQVYP"
BASE COUNT          506 a         1089 c         1036 g          500 t
ORIGIN      
        1 ctttcgttat actccgccca tgagcgatga ccgcctcggc tcgacgctat tcctcgccgc
       61 gctcgttcat ggcgtcgtca ttctcggcgt aacgttcacc gccgctccgt tcggcgacgg
      121 cgaggccttg ccgtccttga acgtgacgct cgttctcaat accgagcgcg aagagctcgc
      181 ggccgacacg gccgatttca tcgcgaatcg tgaccagcgt ggcgccggcg aagccgcccg
      241 tgggctgcgg ccaacgagca ccctgtccgc ggaccaaccc gtgacccagg tcggcgatcc
      301 gttgggcgcc gacgcaaccg acggcactcc gcgcgaagaa acgccgagcg ccgagcaagt
      361 cgtgagtcgc ggtgcgagca gcgaagcgat cgcggcgctg ccgcagccaa ccgacgatcc
      421 ggcactgacg cgccagcgtg cggcggcgct gatccagcaa gccgctcctc agacgacggc
      481 catggagctc gcgctgcgcg cagagctgcc cgacggcgat gacgatgcgc gaacgctgat
      541 cgcgaccccg agcactcgtc gatccgatct cgccgagtac ttggacggct ggcgacggcg
      601 tgtcgagcgc atcggcacgg cgaactatcc gacggggctc ttgggcaaca tcgacgccgg
      661 ccgaccgacg ctcgaggtcg tcatcggcgc cgacggccgt ctcgacgaca tcgtcgtgcg
      721 ccagtcgtcg ggcgacaaag cgctcgatca agccgcgctg aaaattttgc ggctcgctgc
      781 accgttcgcg ccgttgcctg acgagattcg caaggactac gacgtgttgc gcttcgccta
      841 cgagtgggac tttgcgggtg gggctgccag caggcgctga gcgcgccgtc gatgctacga
      901 tagcccatcg tttccgcagg gagctcgagc gccgatgcaa tcgctgcaag accacttctt
      961 gattgcgatg cctgcgatgg cggatcccaa tttcaacgag accgtcacct acatctgcaa
     1021 gcacgacacc gagggcgcgt tcggcatcgt gatcaaccgg ccgagcgact tgtcgctcgg
     1081 cgagatgttg acccagctgg ccatcaatct gacggaccgc aggctggccg accgccccat
     1141 catgcatggc ggtcccgtcg agccggagcg cgggtttgtg ctgcatcgtt cggatcaagc
     1201 gtacgaagcg acgctcgccg ccggctccga aatcaagctg acgtcgtcgc ccgatgttct
     1261 cgcggctctc ggccgcggca cgggtccgga gcctgtgctc gtcgcgcttg gctacgcggg
     1321 ctggggccgc ggccagctcg agatcgagct cggcagcaac acgtggctga ccgtgcctgc
     1381 caatccgacg attatcttcg aaacgccgtg cgagcagcgc tggaccgcgg cgctcggctt
     1441 gccgggcgtc gacatacgcc gcatcacgca ctatgccggt catgcctgac caccggggac
     1501 gcccgcgtgg cgcgcgctag cgaccaccac ctactcgtag cgttcgattt cgggctccgt
     1561 cgcataggca tcgccaccgc gaaccgcgag accggaaccg cttcgccgct gacgacactg
     1621 cgcgtcgagc gcgatctgcc ttgggtggag atcgatcgcg tcatcgaaga ttggcaaccg
     1681 gcgcagctcg tcgtcggcgt gcccgaagga gaaggcgcgg ccgccatcgc gcggcgcgct
     1741 cgcgcgttcg ctgcgggcct ggccgagcgc tacggcctgc ccgtcgcgac cgtcgacgag
     1801 acactgacct ccgtcgcggc cgaaaccgag ctcgcggaaa agcgccgatc cgggtatctt
     1861 cgccgccgca tcggcaaggg cagcgtcgac cgggtcgcgg catgcttgat cgccgagcag
     1921 tggatgagag agcagtgaga ccacggcagt gagctacgag agcaatcgcg gcagcgttta
     1981 cctcgaggat gcgcccatcc tcgcgcagcg cgcgtatccc ggcgagcagt tcgtgctgcg
     2041 agtgcaagcg ccgcgcgcgg cgcgccgcgc gacgccgggc tcgttcgcac acatctcttg
     2101 tgacccgtcg gtcgcgatgc gccggccgct ctcgatcatg cgcgcaagcc cgagcgaggg
     2161 ctggctcgag ttcttgtaca aaccgaaagg ccatgggctc gagatgctcg ggttccggca
     2221 gcccggcgaa gccgtcagcg tgctcgcgcc gatcggccac gggtttaccg tcgatcgtgc
     2281 ccggccgcga ctgctcgcac tcggcggcgg agtcggcatc ccgccgatga tctttctcgc
     2341 cgagcaggtg cgcgccgacc gcacgcttcg gccgctcgtc ctgatgggct ctgaggtgcc
     2401 gttcccgttc gacctagtcg agtctcgcgc accggtgccc ggtgtgaaaa agccggccac
     2461 acacgcagtc gcactgctcg agcagtgggg cgtcccgtcg cggctcgcga gcaacgcgtg
     2521 ccttcccgga gcgcaccgcg gctacatcac cgacctcgcc cgcgacgccc tgcaagcaat
     2581 gaccaacagc gagcgcaccg aagtgcaagt gttcgcgtgc ggcccaacgc cgatgctgaa
     2641 agccgttgcg aagctcgcac gcgacttcga tttgccgtgc caagtcgctc tcgaggagta
     2701 catggcgtgc ggcatcggcg gctgcgccgg ctgcactgtg ctgctgcaaa cgcctgacgg
     2761 cccagccatg aagcgcgtct gcgtagatgg gccggtgttc gacgcccgcc aggtctaccc
     2821 ctagagacgg acgcgtcgtc gcacgcgacg cgcactcggt cattcctccc gctaccgccc
     2881 ggatcgccgg ggctccgcac cgcgctattc accgcgcggg ccgcgtgtgc cattcaaagc
     2941 tgcgcagcgt tgcggagcgc ttctgccgtt cggaagggcg ggcgtatgtt tcgtggcccg
     3001 cttgcagcgg cggtccgagc gcaggaggaa caaccgagcg cgcgtcccaa cgcagcgaag
     3061 acgcgacacg tcctgcttat gggtagacct ggcgggcgtc gaagacgggg ccgtctacgc
     3121 agacgcgctt c
//
LOCUS       DATLEE010000337         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3185049, whole genome shotgun sequence.
ACCESSION   DATLEE010000337 DATLEE010000000
VERSION     DATLEE010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3185049"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VM692_07215"
     CDS             <1..1214
                     /locus_tag="VM692_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HUQ51994.1"
                     /translation="KPTLERMTGQDIPQSVRETQRLSTMSNGQSAFPIIGPLRPFKQR
                     GQSGAWVSDFLPATAAIADELCFVKSLHTEHVNHDPASKFLHTGFQLAGRPSAGAWVS
                     YALGSDNRDLPNFVVMNSGVSQGVPQDAAIWGPGFLPSHHQGVEFRAADDPVLYVNNP
                     QGIDRNDRRAMLDSLAKLAALQHDESNDPEILSRVSQYEMAYRMQASVPEVADISDEP
                     ESVLALYGPDVQKPGTFARNCLIARRLAERGVKYQTLFHLGWDLHLAIKQNLPLRCGE
                     IDRPSAALVMDLKQRGLLDDTLVMFGSEFGRTPFAQGQIDNPLVGRDHHGGCFTWWFA
                     GGGVKAGHTHGETDEFSYNVVKDPVHIHDLNATLLHVLGLDHERLTFRFQGRDYRLTD
                     VHGKVVHEILT"
     gene            1225..>2433
                     /locus_tag="VM692_07220"
     CDS             1225..>2433
                     /locus_tag="VM692_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HUQ51995.1"
                     /translation="MQEFGFFLGRFHVLALHLPIGIVIVAVILDWLARRPRYAPLAAA
                     APMLWALAAISAVLTVALGYLHFGEGGFTGPSAEAHRMWGTITAVVAVGGWWLAARSA
                     GQAGGASVLRLTVGIAMLALISITGHYGGNLTHGTTFLSEYAPSFLRGLIGAAPRRPA
                     PTSVAAADPYLDVVQPLLEQRCGTCHNDDKRNGGFSMGSYDSTLVGGDTGRAIVPGNL
                     EASELIYRTGLPPDDEAFMPAEGKTPLTAEQVAILRWWVGAGAPRGTTVGSIGAPADV
                     EPLLAAQLGLAGPPAAAPGASATADDPLKSSKGDTALVARLSAAGFLVRQISQSDARL
                     VVSVASPGTKLAPEALAALNAAAAAIVDLNLADTGLDDAGLAAIGPLPAATHLRLARN
                     ALTDAGIATLT"
BASE COUNT          365 a          864 c          818 g          386 t
ORIGIN      
        1 acaagccgac gctcgaacgc atgaccggcc aggacattcc gcagtccgtg cgcgagacgc
       61 agcggctctc gacgatgtcg aacggccagt cggcgtttcc gatcatcggg ccgttgcggc
      121 cgttcaagca gcgcggccag agcggcgcgt gggtcagcga cttcttgccg gcgacggccg
      181 cgatcgccga cgagctgtgc ttcgttaaaa gcctgcacac cgagcacgtc aaccacgacc
      241 ccgcgtcgaa gttcttgcac acgggctttc agctcgcggg ccgtccgtcg gccggcgcct
      301 gggtcagcta cgcgctcggc tccgacaacc gcgacttgcc gaacttcgtc gtcatgaact
      361 ccggcgtctc gcaaggcgtg ccgcaggacg ccgcgatctg gggcccgggc ttcctgccct
      421 cgcatcatca aggcgtcgag ttccgcgcgg ccgacgaccc cgtgctgtac gtcaacaacc
      481 cgcaaggcat cgaccgcaac gatcgccgcg cgatgctcga ctccctcgcg aagctcgcgg
      541 cgttgcagca cgacgaatcg aacgacccgg agatcttgtc gcgcgtcagc caatacgaga
      601 tggcgtatcg catgcaggct tcagtgcccg aggtcgccga catctccgac gagccggagt
      661 cggtgctcgc gctgtacggc cccgacgtgc agaagcccgg gacgttcgcg cgcaattgct
      721 tgatcgcgcg ccggctcgcc gaacgcggcg tcaagtatca gacgttgttc cacctgggct
      781 gggacctgca cctcgcgatc aagcagaact tgccgctgcg ctgcggcgag atcgaccggc
      841 cgtccgcggc gctcgtcatg gacttgaagc agcgcggcct gctcgacgac acgctcgtga
      901 tgttcggcag cgagttcggc cgcacgccgt tcgcgcaagg ccagatcgac aatccgctcg
      961 tcggccgcga tcaccacggc ggctgcttca cttggtggtt cgccggcggc ggcgtgaaag
     1021 ccggccacac gcacggcgag acggacgagt tcagctacaa cgtcgtcaaa gaccccgtgc
     1081 acatccacga cttgaacgcg accttgctgc acgtcttggg gctcgaccac gagcgactga
     1141 cgttccgctt ccaaggccgc gactaccgtt tgaccgacgt ccatggcaag gtcgtgcacg
     1201 agatcctgac ctagggcgtt agccatgcaa gagttcggtt tcttcctcgg gcggttccac
     1261 gtcctcgcgc tgcatctgcc gatcggcatc gtgatcgtgg ccgtgatcct cgattggctc
     1321 gcgcgccggc cgcgctatgc cccgctcgcg gcggcggcgc cgatgctctg ggcgcttgcg
     1381 gcgatctcgg ccgtgttgac ggtcgcgctc gggtacttgc acttcggcga gggcggcttc
     1441 acgggcccgt cggcggaagc tcatcgtatg tggggcacga tcacggccgt cgtcgcggtc
     1501 ggcggctggt ggctcgcggc gagaagtgcg ggccaagcgg ggggcgcgag cgtgctgcgg
     1561 ctcaccgtcg gcatcgcgat gctggcgctg atctcgatca cgggccacta cggcggcaac
     1621 ctcacgcacg gcacgacgtt ccttagcgaa tacgcgccgt cgttcctgcg cggcctgatc
     1681 ggcgccgcgc cgcgccgccc ggcgccgacg agcgtcgccg cagcggaccc ctatctcgac
     1741 gtcgtgcagc cgttgctcga gcagcgctgc ggcacctgcc acaacgacga caagcgcaac
     1801 ggcggcttca gcatgggcag ctacgattcg acgctcgtcg gcggcgatac ggggcgcgcc
     1861 atcgtgcccg gcaacctcga agccagcgag ctgatctatc gcacgggttt gccgcccgac
     1921 gacgaggcgt tcatgcccgc cgagggcaag acgcccttga cggccgagca agtcgctatt
     1981 ctgcgttggt gggtcggcgc cggcgcgccg cgcggcacga ccgtcggctc gatcggcgcg
     2041 cctgcggacg tcgagccttt gctcgcggcg cagctcgggc tcgcgggccc cccggcggcc
     2101 gctccgggcg ccagtgccac ggccgacgac cctttaaagt cctcgaaggg ggacacggcg
     2161 ctcgtcgcgc ggctgtcggc ggccggcttt ctcgtgcgcc agatttcgca aagcgacgcg
     2221 cggctcgtcg tcagcgtggc ctcgccgggc acgaagctcg cgcccgaagc gctcgcggcg
     2281 ctgaacgccg ccgcagccgc gatcgtcgac ttgaatctcg ccgacacggg cctcgacgac
     2341 gcggggctcg cggcgatcgg cccgttgccg gcagcgacac atctgcgcct cgcgcgcaac
     2401 gcgctgaccg atgcgggcat cgcgacgctg acg
//
LOCUS       DATLEE010000338         6029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1876182, whole genome shotgun sequence.
ACCESSION   DATLEE010000338 DATLEE010000000
VERSION     DATLEE010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876182"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..467)
                     /locus_tag="VM692_07225"
     CDS             complement(27..467)
                     /locus_tag="VM692_07225"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUQ51996.1"
                     /translation="MTDTSRRNLARKLLCVAALGGLSLYWLAQAQNAATDADRFTGVT
                     RRLEAEGWRVSRRSFEPSARSAWHRHAGGQLIFVESGRARVQDRGGKMLELGVGESHY
                     TAQGIEHWHGAAPATEFHQVALSRGDEAETVWLEKVSDPQYAGR"
     gene            complement(498..935)
                     /locus_tag="VM692_07230"
     CDS             complement(498..935)
                     /locus_tag="VM692_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HUQ51997.1"
                     /translation="MSRFRLVAATFSVFAAGFVAGHWFDFTSEAQAQARGKVFELRTY
                     TAPEGKLNDLVARFRNDTLRIFEKHGMHNVGYWLPTDAPASSNTLVYILAHDSRDAAT
                     KSWAEFRDDPEWKAVAERTQANGPIVTKVDSVFMQATDFSPLK"
     gene            complement(991..1491)
                     /locus_tag="VM692_07235"
     CDS             complement(991..1491)
                     /locus_tag="VM692_07235"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ51998.1"
                     /translation="MQRALNIGKRAAAVAAGILSLVAWAPSSAHHSYAMYDGSVYKVF
                     TGVIVRIVPNAAHFEMHFVPLNETRDALVRDEKGEQLVWVVQMESAAQAFEHGITRES
                     FPQGTVMSMGVHPRRDGKPAGDRGDSGLFQCPKGKVPAPGKHCDSVEGAKAFGEGELP
                     ADNSKR"
     gene            complement(1513..2823)
                     /locus_tag="VM692_07240"
     CDS             complement(1513..2823)
                     /locus_tag="VM692_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ51999.1"
                     /translation="MVGARSAFGAAMSLALLVVFNASAQLQPPQVPGLRGAPFPSGNP
                     PPDLQSFGQGPAWDAKAPPGVEPLARDLFTSKDFYQDREQWLNPRYWRCNSPRQVADM
                     RSGGAGAGTSDPRIGANPPVTARWGDCTKDWARENIVSPYPFKTAADHYAALKADAQK
                     RGGPTRHTYETMPKWDGVYGEYLPDGRRVWNYGRATQVPTFLSILTPEYQQRMVRQLY
                     HEGVDAAHQWSASYCWPEGFMRQWATGPKPQRIIVTPEVVMFVGSGSGNMWRVAHLNR
                     ELPLGQDIPQWYGDTVAFWDGDALIMHTANVQAWTQHTTWEFSYDFETVEILTPARDA
                     AGKFLGIDWETVIYDSEALQQPVRILWHRNYQQSWSEAGRLGWAECTRALYPIDGFAT
                     QVAPGQTIEFKVPDMLDRPWAKIWEENFEQNMDKPKDEIDLGFK"
     gene            3005..3676
                     /locus_tag="VM692_07245"
     CDS             3005..3676
                     /locus_tag="VM692_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52000.1"
                     /translation="MFAKRAKQSSQTKPPKDDGSQTAQSGAGSSADADESLERELAEQ
                     RTLAATLRQQLDAALFKIEILEKSYATQLAEVRDKRASVDEELKEKNEILANLGGGHE
                     HTLRELNDALTVIKVLKAERDKLRKQIAHGGFRQPSERAAREQPTRARALVGGDVGGS
                     ESASSIEDTGAETINALIANTSWAERKPEVGSGHSGAQVKDQEAAPHEEMIAPDLVFT
                     EKDKD"
     gene            complement(3680..4540)
                     /locus_tag="VM692_07250"
     CDS             complement(3680..4540)
                     /locus_tag="VM692_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUQ52001.1"
                     /translation="MPSINDLTIVNSNTAPATKVDHGLLRTLCSPELCDSKNLSVYKR
                     TIQKGKQYDLKAGNDYHLVYAMNSPAKGTVQFKNQSRAVEEGAGVLLVPGESARFDAA
                     GADMDLLHMVVPKPPAGVEDGLPGGPGYFFNRQTLRGLSDASGGRVRRFCAESSVRLE
                     DGSRLTPTNAIQAGEMHYNEGGSSPYHMHKGTAANPDGAAHCYMTFKGRGKVEVADTS
                     EEIEPGTLVYFPPGVPHRLRAHGGTLDYFEIQAWRSFKTTILSEEPLGLKWYYEREKP
                     GAALVEWNQS"
     gene            complement(4663..>6029)
                     /locus_tag="VM692_07255"
     CDS             complement(4663..>6029)
                     /locus_tag="VM692_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ52002.1"
                     /translation="ALNARDGSLIWEYRIDAAIASRGIALYEDKVIFQSNAHLVALNA
                     RTGELEWDVEMKDSRSSSSGPLAANGLILEGMGGCQRYEENKCFISAYDPDTGEQKWR
                     FVTIALDGQPGGDSWGDLPNLYRAGGETWITGSYDPELNLTYWGTAQAKPWMPVSRGM
                     SSRDVGLYTNSTVAIDASTGKLAWHYAHAPGEALDLDVVFERVLVDSGDDKWVLSAGK
                     DGVLWKHDRRDGTYLGHVETLFQNVWASFDRETGTPRYREDILEHGVGDWVDGCPSTE
                     GGKNWQAMSFHRPSRRLIIPLSQSCISIRAQAIAQTPGGGSAGGADRRYYEMPGSDGN
                     IGRLAAFNVDTLKEEWAIQQRAPFLTGVLSTAGGVAFVGDLDREFKAVDVRNGRILWR
                     TRLATSVQGFPVSFEVDGKQYIAVTTGLGGGSPRLVPSLIAPEIQAPPRGHALYVFAL
                     PDSR"
BASE COUNT          995 a         2026 c         1937 g         1071 t
ORIGIN      
        1 cgcaatacgc gggccagtcg aggtagctag cgccccgcat attgtgggtc gctgaccttc
       61 tcgagccaca cggtctcggc ctcatcgccg cggctcaacg caacttgatg gaactcggta
      121 gccggtgctg cgccgtgcca gtgctcgatg ccttgcgccg tgtagtgact ttcaccgacg
      181 ccaagctcca gcatttttcc gccgcggtct tggacacgcg cgcggccgga ctcgacgaat
      241 atcagctgac cacccgcgtg ccgatgccaa gccgagcgtg ccgacggctc gaacgagcgg
      301 cgggagacgc gccacccttc ggcttcgagc cggcgcgtca cacccgtgaa acgatcggcg
      361 tcggttgcgg cgttctgagc ttgcgcgagc caatacagcg acagtccgcc gagcgcggcg
      421 acgcagagca gcttccgtgc gaggtttctg cgcgaagtgt ccgtcatcgc caacctcctc
      481 ggctcggagc cgagccgcta cttcagcggc gagaaatccg tcgcctgcat gaacacggaa
      541 tcgactttcg tgacgatcgg cccgttcgcc tgcgtgcgct cggcgacggc cttccattcc
      601 gggtcgtcgc ggaactcggc ccagctcttg gtcgccgcat cgcggctgtc gtgcgccagg
      661 atgtagacga gcgtgttgct cgacgcgggc gcgtcggtcg ggagccagta gccgacattg
      721 tgcatgccgt gcttctcgaa gatccgcagc gtgtcgttgc ggaagcgcgc gacgaggtcg
      781 ttcaacttgc cttccggcgc cgtgtaggta cgcagctcga agactttccc gcgcgcttgc
      841 gcctgtgcct cgctggtgaa gtcgaaccaa tggccggcga caaagcccgc ggcaaacacc
      901 gagaacgtcg cggcaaccag gcgaaatcgg ctcatcgtat ctctccgcat caatgggttg
      961 aggtgcgttc tcgagaagct cgcaagctcg tcagcgcttg gagttgtccg ccggcagctc
     1021 gccttcgccg aacgccttcg cgccttcgac ggaatcgcaa tgcttgccgg gcgccgggac
     1081 cttgcccttc gggcattgga acagccccga gtcgccgcga tcgccggccg gcttgccgtc
     1141 gcgcctcggg tgcacgccca tgctcatgac ggtgccttgc gggaagctct cgcgcgtgat
     1201 gccgtgctcg aacgcctggg ccgcgctctc catctgcacg acccacacga gctgctcgcc
     1261 tttctcgtcg cgcaccagcg cgtcgcgtgt ctcgttgagc ggcacgaagt gcatctcgaa
     1321 gtgcgcggcg ttcggcacga tacgcacgat gacgcccgtg aatactttat aaacgctgcc
     1381 gtcgtacatc gcgtacgagt gatgtgcgct cgacggcgcc catgccacga gcgagagaat
     1441 gcccgctgcc accgctgccg cgcgcttacc gatgttcaaa gctctctgca ttgctgcgaa
     1501 tcctctgcct gcttacttga agccgaggtc gatctcgtcc ttcggcttgt ccatgttttg
     1561 ctcgaagttc tcttcccaga tcttcgccca aggccgatcg agcatgtcgg gcaccttgaa
     1621 ctcgatcgtt tgcccgggcg cgacctgcgt cgcgaacccg tcgatcgggt acagcgcgcg
     1681 cgtgcactcg gcccagccga gccggcccgc ttcgctccag ctctgctgat agttgcggtg
     1741 ccagaggatg cgcacgggct gctgcagcgc ctcggagtcg tagatcacgg tctcccagtc
     1801 gatgccgaga aacttgccgg ctgcgtcgcg cgccggtgtg aggatctcga cggtctcgaa
     1861 gtcgtagctg aactcccacg tcgtgtgctg cgtccacgct tggacgttcg ccgtgtgcat
     1921 gatcaacgca tcgccatccc agaacgcgac ggtgtcgccg taccactgcg gaatgtcttg
     1981 gccgagcggc agctcgcggt tgaggtgcgc gacgcgccac atgttgccgc tgccgctgcc
     2041 gacgaacatg acgacttcgg gcgtcacgat gatgcgctgc ggcttcggcc ccgtggccca
     2101 ctgccgcatg aagccctcgg gccagcagta cgacgccgac cactgatgcg ccgcgtcgac
     2161 gccttcgtga tagagctgcc gcaccatgcg ctgttgatat tcgggcgtga ggatcgacag
     2221 gaatgtcggc acttgcgtcg cgcggccgta attccaaacg cgtcggccgt cgggcagata
     2281 ctcgccgtag acgccgtccc acttcggcat cgtctcgtac gtgtggcgtg tcgggccgcc
     2341 gcgcttctgc gcgtcggcct tcagcgccgc gtagtggtcc gccgccgtct tgaacgggta
     2401 cgggctgacg atgttctcgc gcgcccaatc cttcgtgcag tcgccccagc gagccgtgac
     2461 gggcgggttt gcaccgatgc gcgggtcgga cgtgccggca ccggcgccgc cgctgcgcat
     2521 gtcggcgact tggcgcgggc tgttgcagcg ccagtaacgc gggttcagcc actgctcgcg
     2581 gtcttggtag aagtccttgc tcgtgaacag atcacgcgcg agcggctcga cgccgggcgg
     2641 tgccttcgcg tcccacgcgg gcccctgacc gaagctctgc agatccggcg gcggattgcc
     2701 ggatgggaac ggtgcaccgc gcaagcccgg tacctgcggc ggctgcaact gtgccgatgc
     2761 gttgaaaaca accagtagcg ccagcgacat tgcggcgccg aacgcggagc gagctccaac
     2821 catcaattcc ccctgagcct cggccgcccg tggccgaagg ctcggggcag tacagccggt
     2881 cctgtgactt gggtcaaggc tgcgccacgc cgcgccgctg cgcggccatg cgctgtgatc
     2941 tgtgtccggg ttccgtgcgt gctgcgcgac tacgcttgct cgcgacgtat cgggccaagt
     3001 caccatgttc gctaagcgcg ccaagcagtc ttctcaaacg aagcccccga aggacgacgg
     3061 cagccaaacc gcacagtccg gggcgggctc gtccgccgac gccgacgaaa gcctcgagcg
     3121 cgagctcgca gagcagcgca cgctcgcggc gacgctgcgc cagcaactcg acgcggcact
     3181 gttcaagatc gaaatccttg agaaaagtta tgcgactcag ctcgccgagg tccgcgacaa
     3241 gcgcgcgtcg gtcgacgaag agctcaagga aaagaacgaa atcctcgcga acctcggcgg
     3301 cggacacgag cacacgctac gcgagctcaa cgatgcgctg accgtgatca aagtcctcaa
     3361 ggccgagcgc gacaagctgc gcaagcagat tgcgcacggt ggctttcgtc agccgagcga
     3421 gcgcgcggcg cgcgagcaac cgacgcgcgc ccgcgccttg gtcggcggcg acgtgggcgg
     3481 gagcgagagc gcgagctcga tcgaagacac gggcgccgaa acgatcaacg cgttgatcgc
     3541 caacacgagc tgggccgaga gaaagcccga ggtcggctcc ggccactccg gcgctcaagt
     3601 caaagaccag gaagcggctc cgcacgaaga gatgatcgcg ccggatctcg tgttcacgga
     3661 gaaggacaag gactgagcgt cagctctggt tccactccac gagcgccgca ccgggcttct
     3721 cgcgttcgta gtaccactta agccccagcg gctcctcgct caagatcgtc gtcttgaagc
     3781 tgcgccatgc ttgaatctcg aagtaatcga gcgtgccgcc gtgcgcccgt agccggtgcg
     3841 ggacaccagg cgggaagtac acgagcgtgc ccggctcgat ctcttcggac gtatcggcga
     3901 cctcgacctt gccgcggccc ttgaacgtca tgtagcagtg cgccgcgccg tcgggatttg
     3961 cggcggtgcc cttatgcatg tggtacggcg acgagccgcc ctcgttgtag tgcatctcgc
     4021 cggcttggat cgcgttcgtc ggcgtcaagc ggctgccgtc ctcgagtcgg acgctcgatt
     4081 cggcgcagaa acgccgcacg cggccgccgc tcgcgtcgct caagccgcgc agcgtctgac
     4141 ggttgaagaa ataacccggc ccgccgggta agccgtcctc gacgccggcg ggcggcttcg
     4201 gcacgaccat gtgcagcaaa tccatgtcgg cgccggcggc atcgaaacgg gccgactcgc
     4261 caggcaccaa caacacgccc gcgccctctt cgaccgctcg gctctggttc ttgaattgca
     4321 cggtgccctt ggccggcgag ttcatcgcat agaccaagtg ataatcgttg ccggctttca
     4381 ggtcgtactg cttgcccttt tgaatcgtgc gcttatagac gctgagattt ttcgagtcgc
     4441 aaagctcggg gctgcacagc gtgcgtaaca agccgtggtc gaccttcgtc gcgggggcag
     4501 tgttcgagtt gacgatcgtc aggtcattga tcgagggcat cggtcccaag ctcctaaaca
     4561 agcggcgttg acaggggcca agatgatacg tgcaaaccgc gcgcgcgtgc gactcggcgg
     4621 cccggcgaac cgccgggttt gtctcgggct caataaccgc cgttagcgcg aatccggcag
     4681 cgcgaacacg tacagcgcat gaccgcgcgg cggcgcctgg atctcgggcg caatcagcga
     4741 cggcacgagc cgcgggctgc cgccgcccaa ccccgtcgtg acggcgatgt actgcttgcc
     4801 gtcgacctcg aagctgactg ggaaaccttg caccgacgtc gcgaggcgcg tccgccagag
     4861 gatgcggccg ttgcgcacgt cgacggcttt gaactcgcgg tcgagatcgc cgacgaacgc
     4921 gacgccgccg gccgtcgaca acacgcccgt cagaaacggc gcgcgttgct ggatcgccca
     4981 ctcctctttc agcgtgtcga cgttgaacgc cgcgagccgc ccaatgttgc cgtcggaacc
     5041 cggcatctcg tagtagcgcc ggtccgcgcc gccggcgctg ccgccgcccg gggtttgtgc
     5101 aatggcctgg gcgcgaatcg agatgcagct ctgcgagagc ggaatgatca agcggcgtga
     5161 cggccgatga aagctcatcg cttgccaatt cttgccgcct tcggtgctcg ggcagccgtc
     5221 gacccagtcg ccgacgccgt gctcgagaat gtcttcgcga tagcgcggcg tgccggtctc
     5281 gcgatcgaag ctcgcccaca cgttttgaaa cagcgtctcg acgtggccga gatacgtgcc
     5341 gtcgcgccga tcgtgcttcc agagcacgcc gtccttaccg gccgacaaca cccacttgtc
     5401 gtcacccgaa tccacgagca cgcgctcgaa cacgacgtcc aagtcgagcg cttcgccggg
     5461 tgcgtgcgcg tagtgccacg cgagcttgcc ggtgctcgcg tcgatcgcga cggtcgagtt
     5521 cgtatagagg ccgacgtcgc gcgagctcat gccgcggctt acgggcatcc aaggtttcgc
     5581 ctgcgccgtg ccccaatatg tgagattgag ctcgggatcg tagctgccgg tgatccaagt
     5641 ctcgccgccg gcgcggtaca gattcggcaa gtcgccccag ctgtcgccgc cgggctggcc
     5701 gtcgagcgcg atcgtcacga agcgccactt ctgctcgccg gtgtcgggat cgtaagcgct
     5761 gatgaagcac ttgttctcct cgtagcgctg gcagccgccc atgccttcga ggatcagccc
     5821 gttcgcggcg agcgggccgc tgctgctcga gcggctgtcc ttcatctcga cgtcccactc
     5881 gagctcgccg gtccgcgcgt tcaacgcgac caagtgcgcg ttactttgga agatgacctt
     5941 gtcttcgtag agggcgatac cgcgcgatgc aatcgccgcg tcgatgcggt attcccagat
     6001 caggctgccg tcgcgagcgt tgagcgcct
//
LOCUS       DATLEE010000339         4627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5604774, whole genome shotgun sequence.
ACCESSION   DATLEE010000339 DATLEE010000000
VERSION     DATLEE010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5604774"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VM692_07260"
     CDS             complement(<1..1043)
                     /locus_tag="VM692_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HUQ52003.1"
                     /translation="MPQHYHHGLVGLSVVVAILASYTALTLATRLRVTSGWPARAWLI
                     GGGFAMGIGIWAMHFVGMLALALPLPIAYDVTITLLSMLIAIVVSTFALRIAGRPMLP
                     PRRLAVAGVAMGTGICSMHYVGMAAIELESPIHYDAYWVAVSFAIAIAASFAALGVVF
                     KAPVQDGRWALLRLPLGGVAMGFAIAGMHYAGMTAAHFPVAAAGASMEPMTNVTTGWL
                     AGTVIMTTVFILLATLLIAFIDARAAARRVRMQVSLAKEQQSNLAKDQFLATLGHELR
                     NPLAAITNASFLLNCVEPRSAEWAFAHGVIERQSSHLKRIVDDLLDVGRAISGKMALD
                     AQPVDLATTVESAL"
     gene            1159..1617
                     /locus_tag="VM692_07265"
     CDS             1159..1617
                     /locus_tag="VM692_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HUQ52004.1"
                     /translation="MTATVAAAFIHHAAAFLVVAALMVELVVLKQELTVQSARSVLRM
                     DMVYGVAATLLLIVGFFRVFHTEKGMAYYFASGSFLTKLTLFIIVGVLSIYPTVKFLG
                     WRRALHEQRVPEFPAGVRRKVRMLIHIELTLLFVIMLMAVMMARGIWYFG"
     gene            complement(1624..2916)
                     /locus_tag="VM692_07270"
     CDS             complement(1624..2916)
                     /locus_tag="VM692_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52005.1"
                     /translation="MSAAAPGIERRGPRARTPVRTQFPIWFAATEGARAALGSIRAHS
                     FRSLLTTLGIVIGVGSVIAMISIIQGLSVMIGDQFAGLGANGVTVSSFTSREAFLAGR
                     IARLSEADYNLIRFRVEGLESITPIMQTNGQVRSGSKATATQVIGSTYAYQGVAQYYT
                     SRGRFVSDSDNQTRRRVAVIGEQVRADLALPENPLGEYIQFNGEWLRIIGLLEEKGEI
                     FGQSQDNQLIIPFNTLASLQGNQRQRDLWIRLTIRDLSELDQVSATITRLLRTAHNIG
                     REDEDDFRVQSAQQLQDTVEGILNTVTVVMSGIVSISLLVGGIGIMNIMLVSVTERTR
                     EIGICKAIGAKRHHILLQFLLEALVLCLFGGLIGLVLGYGIGALAASFIPGFPPAYTP
                     WWAVALALGFSGVVGVVFGILPAAKAANLDPISSLRYE"
     gene            complement(2891..3592)
                     /locus_tag="VM692_07275"
     CDS             complement(2891..3592)
                     /locus_tag="VM692_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ52006.1"
                     /translation="MPVIELAAISKHYEMGQQLIKAVDKLDLTIDYNEYIVFIGSSGS
                     GKSTLMNIIGCLDTPTSGRYVLNQKDVSGMEENELADIRNQEIGFIFQSFNLLPRASA
                     LKNVMQPLIYRGMTLSERKAEAERVLERVGLKDRADHLPNQLSGGQRQRVAIARALVT
                     NPSILLADEPTGNLDSQTSVEILALFDELHSDGQTIVVVTHEPDVAAHGQRIVRLKDG
                     KVVEDQRNERGRTRH"
     gene            complement(3598..>4627)
                     /locus_tag="VM692_07280"
     CDS             complement(3598..>4627)
                     /locus_tag="VM692_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUQ52007.1"
                     /translation="GIYVDEGQKVKKGELVLQIDDQRLRAAVEQGQASVRMQEIAIQR
                     QQLQVANLRTQWERSRGLHERKLIDENSFVTATNNLEIAEVDLMSSKESLEQARAQLD
                     QQDDRLAKTRVYSPIDGTVTTLDIKVGETAIASSTNIPGSSLMTIANPASIHTEVNVD
                     EADIANVSVGQMARVYAIAYPDQPVEGVVDSIAVSAKVAEGQQGQSFAIKIRLLDPEK
                     ITLRPGMTCRAEIFTATKGDVLAAPIQAILVDEDLTTDVVRRDVFVNRNGHASRVPVQ
                     VGLSDDTYQEIVSGLAVGDEVVTGPDRVLRALEDGDRIAIAENNRSNRATQGDSNGNR
                     NRATVVAE"
BASE COUNT          768 a         1488 c         1526 g          845 t
ORIGIN      
        1 agcgcgcttt cgaccgtcgt cgccaggtcg acgggctgcg cgtcgagcgc catcttgccg
       61 ctgatggcac ggccgacgtc gagcaaatcg tcgacgatgc gcttcaagtg cgacgactgc
      121 cgttcgatga cgccgtgcgc aaacgcccat tccgcgcttc tgggctcgac gcagttcagc
      181 aggaagctcg cgttcgtgat cgcagcaagc gggttgcgca gctcgtggcc aagcgtcgca
      241 agaaactggt ccttggcgag attcgactgc tgctccttgg cgagcgaaac ctgcatgcgc
      301 acgcggcgtg cggctgcacg cgcatcgatg aatgcgatca gcagcgttgc gagcaggatg
      361 aagaccgtcg tcatgatgac cgtgcccgca agccaccccg tggtcacgtt cgtcatgggc
      421 tccatggagg cgcccgcggc ggccacgggg aaatgcgcgg cggtcatgcc ggcgtagtgc
      481 atgccggcga tcgcaaatcc catggcgacg ccgccgagcg gcagccgcag cagcgcccaa
      541 cgtccgtcct gcactggcgc cttgaacacg acgccgagag ccgcgaacga ggcggcgatc
      601 gcaatcgcaa aggacaccgc gacccagtac gcgtcgtagt ggatcggcga ctcgagctcg
      661 atggcggcca tgccgacgta gtgcatcgag cagatgcccg tgcccatcgc tacgccggcg
      721 acggccagac gtctcggtgg cagcatcggg cgaccggcaa tccgcaatgc gaacgtggaa
      781 acgacgattg cgatcaacat cgacagcaat gtgatcgtca cgtcgtaagc gatcggcagc
      841 ggcagcgcga gagcgagcat accgacgaaa tgcatcgccc agattccgat gcccatggcg
      901 aaaccgccgc cgatcagcca ggcgcgcgcc ggccaccccg acgtcacgcg cagacgcgtg
      961 gccagcgtta gagcggtgta agaggcgaga atcgccacga ccaccgagag accgacgagg
     1021 ccgtgatggt agtgctgagg cataccaagc cgtggctgat cgggcgccca atataggctg
     1081 cttccgacgt cccgtcattc tcaaaagcgt ctcgttttgc cgacacaccg cgccgcggct
     1141 cgcgtagact cggcagccat gacggcgacg gttgcagcgg ctttcattca ccacgcggcg
     1201 gcgttcctcg tcgtggcggc cctcatggtc gagctcgtcg tgctgaagca ggagctcacg
     1261 gtgcaatcgg cgcgcagcgt gctgcgcatg gacatggtgt acggcgtcgc cgcgacgctg
     1321 ctgctcatcg tcggcttctt ccgcgtgttc cacaccgaga aaggcatggc gtactacttc
     1381 gcgagcggct cgttcctcac gaagctcacg ctgttcatca tcgtcggcgt gttgtcgatc
     1441 tacccgacgg tcaagttctt gggctggcgc cgcgccttgc acgagcagcg cgtgcccgag
     1501 ttcccggcgg gcgtgcgccg caaagtgcgc atgctgatcc acatcgagct cacgctgctg
     1561 ttcgtcatca tgctgatggc cgtgatgatg gcgcgcggaa tctggtactt cggctaggcg
     1621 cctttattcg tagcgcaggg agctgatcgg atcgaggttc gcggccttgg cggccggcaa
     1681 gatgccgaac acgacgccga cgacgccgga gaatcccagc gccagcgcga cggcccacca
     1741 cggcgtgtac gcgggcggga agcccggaat gaacgatgcc gcgagcgcgc cgatgccgta
     1801 gccgagcacg aggcctatga ggccgccgaa caggcagagc acgagcgcct ccagtaagaa
     1861 ctgcagcaag atgtgatggc gcttagcgcc gatggctttg cagatgccga tctcgcgcgt
     1921 ccgctccgtg accgacacca gcatgatgtt catgatgccg atgccgccga cgagcaacga
     1981 gatgctgacg atgccgctca tgacgacggt caccgtgttc agaatgccct cgacggtgtc
     2041 ctgcagctgc tgggccgatt ggacgcggaa atcgtcctcg tcctcgcgcc cgatgttgtg
     2101 cgcggtgcgc aggagccgcg tgatcgtcgc gctgacttgg tcgagctcgc tcaagtcgcg
     2161 aatcgtcaga cggatccaca agtcgcgctg gcgctgattg ccctggaggc tcgcgagcgt
     2221 gttgaacggg atgatcagct ggttgtcctg gctctggccg aagatctcgc ccttctcctc
     2281 gagcaagccg atgatcctga gccattcgcc gttgaactgg atgtactcgc cgagcggatt
     2341 ctcgggcagc gccaagtcgg cgcggacctg ctcgccgatc acggcgacgc ggcggcgcgt
     2401 ctgattgtcg ctgtcggaca cgaagcggcc gcgcgacgtg tagtactgcg cgaccccttg
     2461 gtacgcgtac gtcgagccga tgacttgcgt cgccgtcgct ttcgagccgg accggacttg
     2521 cccgttcgtc tgcatgatcg gcgtgatcga ctcgaggcct tcgacgcgga agcggatcag
     2581 gttgtagtcg gcttcgctga gccgagcgat gcgtcccgcg aggaatgcct cgcgcgacgt
     2641 gaacgacgac acggtcacgc cgttcgcgcc gaggcccgcg aactggtcgc cgatcatgac
     2701 ggagagcccc tggatgatcg aaatcatcgc gatgaccgag ccgacgccga tcacgatgcc
     2761 gagcgtcgtc aaaaggctgc ggaagctgtg agcccggatc gatcccaatg cggctcgcgc
     2821 gccttcggtg gcggcaaacc agatcgggaa ctgcgtgcgc acgggcgtgc gcgcgcgcgg
     2881 cccgcgtcgc tcaatgccgg gtgcggccgc gctcattgcg ctggtcctcc acgactttgc
     2941 cgtccttcaa gcggacgatg cgctgcccgt gcgccgcgac gtcgggctcg tgcgtcacga
     3001 cgacgatcgt ttggccgtcg ctgtgcagct cgtcgaacaa cgcgaggatc tcgacgctcg
     3061 tctgcgagtc cagatttccc gtcggctcgt cggctagcag gatcgacggg ttcgtgacga
     3121 gcgcgcgcgc gatcgcgacg cgctgccgct gaccgcccga aagctgattc ggcaagtgat
     3181 cggcccggtc cttgaggccg actcgctcga gcacgcgctc ggcctcggcc ttgcgctccg
     3241 acagcgtcat gccgcgatag atcaacggct gcatgacgtt cttcagcgcg ctcgcgcgcg
     3301 gcagcaagtt gaagctctgg aagatgaagc cgatctcctg attgcggatg tcggcgagct
     3361 cgttctcttc catgccgctg acgtctttct ggttcagcac gtagcggccc gacgtcggcg
     3421 tatcgagaca gccgatgatg ttcatcagcg tcgacttgcc cgagcccgac gagccgatga
     3481 acacgatgta ctcgttgtag tcgatcgtaa gatcgagctt gtcgacggcc ttgatgagct
     3541 gctggcccat ctcgtagtgc ttgctgatcg ctgcgagctc gatcaccggc atcggcgcta
     3601 ctccgcgacg acggtggcgc ggttgcggtt gccgttgcta tcgccctggg tggcgcggtt
     3661 gctgcgattg ttctcggcga tcgcgatccg gtcgccgtcc tcgagcgcgc gcaagacgcg
     3721 gtcgggtccc gtgacgacct cgtcgccgac agcaaggccg ctgacgatct cttgatacgt
     3781 atcgtcggac agcccaacct gcacgggcac cctcgacgca tgaccgttgc ggttcacgaa
     3841 gacgtcgcgg cgcacgacgt cggtcgtgag gtcctcgtcg acgaggatcg cttggatcgg
     3901 cgccgcgagc acgtcgccct tcgtcgcggt gaaaatctcg gcgcggcacg tcatgccggg
     3961 gcgcaacgtg atcttctcgg gatcgagcag gcgaatcttg atcgcaaagc tctggccctg
     4021 ctggccttcg gcgaccttgg ccgacacggc gatcgagtcg acgacgcctt cgacgggctg
     4081 atcggggtac gcgatcgcgt agacgcgcgc catctggccg acggagacgt tggcgatgtc
     4141 ggcctcgtcg acgttgacct cggtgtgaat gctcgcgggg ttcgcgatcg tcatgaggct
     4201 cgagcccggg atgttcgtcg agctcgcgat cgccgtttcg ccgaccttga tgtcgagcgt
     4261 cgtgaccgtg ccgtcgatag gcgagtagac gcgcgtcttg gcaaggcggt cgtcctgctg
     4321 gtcgagctgc gcgcgcgctt gctcgagcga ttccttgctc gacatgagat cgacctccgc
     4381 gatctcgagg ttgttcgtcg cggtcacgaa gctgttctcg tcgatgagct tgcgctcgtg
     4441 caagccgcgc gagcgctccc attgcgtgcg caggttcgcg acttgcagct gctgccgctg
     4501 aatcgcgatt tcctgcatgc gcacgctggc ctgcccctgc tcgacggcgg cacgaaggcg
     4561 ttgatcgtcg atctgcagca cgagctcgcc tttcttgacc ttctgcccct cgtcgacgta
     4621 gatgccc
//
LOCUS       DATLEE010000340         4002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5605923, whole genome shotgun sequence.
ACCESSION   DATLEE010000340 DATLEE010000000
VERSION     DATLEE010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5605923"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VM692_07285"
     CDS             <1..339
                     /locus_tag="VM692_07285"
                     /inference="COORDINATES: protein motif:HMM:NF018693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52008.1"
                     /translation="PSYMAGGGLKRRFWMGSQDFYVDLGGVAFLMTRQDVNHNQPFPG
                     ILPAATFGFKRVALNVTYMPEAAVDRVTNSKKYDPAMEGVFFLQLKLDASLFGLGRHN
                     RPFFAEAGAD"
     gene            447..1226
                     /locus_tag="VM692_07290"
     CDS             447..1226
                     /locus_tag="VM692_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HUQ52009.1"
                     /translation="MKRFQWSVIALASVLLADSASAAFLVSEKEVLRQARVEWLSMKR
                     HVPLESRAGVQNYVECVANNVLAALPKEATDNIDWEVVVFDEDEINAFADPNGKIGVF
                     NGILRVADTPDALAAVIGHEIAHATQGHVMVRARKNARQEIWATIGGAATGAGDMWRQ
                     GLAIMSGLPFAREQETEADLVGLDYMANAGFDPRAAVYLWKNMAAAKLAQGREQVPEF
                     LSTHPSDSARIDNMIKSLTPALIKFNAAREAGHRPACTTAG"
     gene            complement(1247..1954)
                     /locus_tag="VM692_07295"
     CDS             complement(1247..1954)
                     /locus_tag="VM692_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52010.1"
                     /translation="MRLTIITAALLVASYPAYGQRNARNETEAVERACTVEDCFLEGQ
                     VRDFEVIDRTHVIVYVGAQRCAFHVELRGALCDLSFAPELYFRRANEVPMIYTSRPDN
                     GIDTPNAVTPRTGNRSSDFDQFELQQRERRDLRVCKNDLTIQVHGGRWTETQSNAGTD
                     RFGNPNSDCRISNVTSITDDQLLEFYVGRGVVAPVPPMGTGQIEVGEQEERADKEAAS
                     PAPSRAEPGQRGRRKAE"
     gene            complement(2036..2977)
                     /gene="glpQ"
                     /locus_tag="VM692_07300"
     CDS             complement(2036..2977)
                     /gene="glpQ"
                     /locus_tag="VM692_07300"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: protein motif:HMM:NF008354.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HUQ52011.1"
                     /translation="MTPAPIVIAHRGASGYLPEHTLAAKALAHGLGADFLEQDVVATR
                     DGTLVVLHDLYLDDVTDVAQRFPKRRREDGRHYVVDFDLDELKTLQVFERRLPGSAAA
                     KYPERFPAEVGIPGIVSLADELRFIQGLNKSTGRTVGIYPEIKEPEWHRRAGIDLAAR
                     LLAELAAFGYKRAADPVFVQCFDAAELVRVKQELSSDLKLIQLVGAEAKYEELLTPVG
                     LRRVAAYASGLGPHHAQLATGAAGRRPQLTPLTQFAQDCGLALHPYTFRRDDLPAYAR
                     SLEEWLEFFIVEARVEGLFCDHPDVAVRVRAARPKVQ"
     gene            complement(2993..3445)
                     /locus_tag="VM692_07305"
     CDS             complement(2993..3445)
                     /locus_tag="VM692_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52012.1"
                     /translation="MSAKNPIRIYVTHAFASHPDYHRVFEYLEALPNFFYRNCSVPDH
                     LPATGGKEALKEEYRTQMKTAEVVVVLSSLYIENEYWATYQMDAAQASNLPLVAMEPF
                     GVNVKVPAEVAKRAAEVIGWNERLIIDAVRRQARHEDTQRWETIEFKL"
     gene            3600..>4002
                     /locus_tag="VM692_07310"
     CDS             3600..>4002
                     /locus_tag="VM692_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HUQ52013.1"
                     /translation="MRLLLYNIRYGLGLGTALHWPLPGVGYLLGNRTNLQRITDFIKS
                     QDPDVVGLIEVDTGSIRTRSVNQADVIARALGHYSVDQCKYGANSLNQRLPILRKQGN
                     AFLAAPRVHGERFHYFDKGIKRLIIELELEGC"
BASE COUNT          715 a         1256 c         1259 g          772 t
ORIGIN      
        1 ccgtcctata tggccggcgg cggcctgaaa cggcggttct ggatgggctc gcaggacttc
       61 tacgtcgact tgggtggcgt cgcctttcta atgacgcgtc aagacgtcaa ccacaaccag
      121 ccgttcccgg gaattcttcc ggcggcgacg ttcggcttca aacgtgtggc gctcaatgtg
      181 acctacatgc cggaagccgc ggtggatcgt gtcaccaact cgaagaagta cgatccggcg
      241 atggaaggcg tctttttcct tcaactcaag ctcgacgcca gcctgttcgg gcttggtcgg
      301 cataacaggc cattcttcgc ggaagccggc gccgactgag cgggcagccc tcactccggg
      361 tcatttcctc gtgtgctatc gttgggcgaa ctgtgggctc gcccgggcac tctaacgcat
      421 acgatccgtc gacgaggaac gaatcgatga agcgttttca atggtccgtc atcgctctgg
      481 cgtcggtcct attggccgac tcggctagcg ccgctttctt ggtttccgag aaagaagttt
      541 tgcgccaagc gcgggtcgag tggctgtcga tgaagcggca cgtgccgctc gagtctcggg
      601 ccggagtcca gaattacgtc gagtgcgtgg ccaacaacgt ccttgcggcc ctgccgaaag
      661 aagcgacgga caacatcgat tgggaagtcg tcgtgttcga cgaggatgag atcaacgcct
      721 ttgcggatcc gaacggcaag atcggcgtgt tcaacggcat tttgcgcgtg gccgatacgc
      781 ccgacgcact ggccgccgtc atcggccatg aaatcgcgca cgcgacgcag ggacacgtca
      841 tggttcgtgc gcgcaagaac gctcgtcagg aaatctgggc gacgatcggc ggcgcggcca
      901 cgggcgccgg cgacatgtgg cgccagggtc tcgcgatcat gagcggttta ccgtttgcgc
      961 gcgagcagga gaccgaagcc gatctcgtgg gcctcgatta catggcgaac gcggggttcg
     1021 atccgcgggc agcggtctac ttatggaaga acatggcggc cgcgaagctc gcgcagggac
     1081 gtgagcaagt gccggaattc ttgtcgactc acccgtccga ctcggcccgc atcgacaaca
     1141 tgatcaagtc gctgacgccg gcgctgatca agttcaacgc cgcgcgcgag gcggggcatc
     1201 gcccggcctg cacgacggcc ggctgagcgc agcgccgctc ggccccctac tctgccttcc
     1261 tgcggccgcg ctggccgggc tccgcgcgcg acggcgcagg agatgcggct tccttgtccg
     1321 cgcgctcctc ttgctcgccg acctcgattt gacccgtacc catcggcggc acgggcgcaa
     1381 cgactccgcg gcccacgtag aactccagga gctggtcatc ggtaatcgaa gtgacgttcg
     1441 agatgcggca gtctgagttc gggttgccga agcggtcggt tcctgcgttg gattgcgttt
     1501 cggtccaccg accgccgtga acctgaatcg tcaggtcatt cttgcaaacg cgcagatcgc
     1561 ggcgctcgcg ttgctggagc tcgaactggt cgaagtcgct cgagcggttt ccggtccttg
     1621 gcgtgaccgc gttcggagta tcgatgccgt tgtcgggccg actcgtgtaa atcatcggaa
     1681 cctcgttcgc tctacggaag tagagctccg gtgcgaacga gagatcgcag agcgcgccgc
     1741 gcaactcgac atggaaagcg cagcgttgcg cgccgacgta gacgatcacg tgcgtgcgat
     1801 cgatgacttc gaagtcgcgg acctggccct cgaggaagca atcctcgacc gtgcaggcgc
     1861 gctcgacggc ctcggtttcg ttcctggcat tacgttgacc gtacgcagga taagacgcga
     1921 ccagcaaggc cgctgtgatg atagtgagcc gcatgatcgc ttccccccag ttcctgaggt
     1981 tgaagttcac tctaggcagt tttccggggg ctgtccacgc cgtttcgagc cgcgctcatt
     2041 gaactttcgg ccgggcggcg cggacacgca ccgcaacgtc ggggtggtcg caaaacagcc
     2101 cttcgacgcg agcttcgacg atgaagaatt ccagccactc ctcgagcgag cgcgcgtacg
     2161 ccggcagatc gtcgcgccgg aaggtgtacg gatgcagcgc gagcccgcag tcctgcgcga
     2221 actgcgtcag cggcgtcagt tgcggccgcc gacccgcggc cccggtggcg agctgcgcat
     2281 gatgcgggcc cagacccgac gcgtaggccg cgacgcgccg tagccccacc ggtgtcagca
     2341 actcctcgta cttggcttcc gcgccgacga gctggatcag tttgagatcc gagctcagct
     2401 cctgtttgac gcgcacgagc tcggcggcgt cgaagcactg gacgaacacg gggtccgccg
     2461 cacgcttgta gccgaaggcc gccagctcgg ccaacagccg cgctgcgaga tctatccccg
     2521 cgcggcggtg ccactcgggc tccttgattt cgggataaat gcccacggta cggcccgtcg
     2581 atttgttcag gccttggatg aaccgcagct cgtcggccaa cgagacgatg cccggaattc
     2641 ccacctcggc cgggaagcgt tccggatact ttgcggcggc tgagcccggc aaccgacgct
     2701 cgaacacttg cagcgtcttg agctcgtcta agtcgaagtc gacgacgtag tgtcgcccgt
     2761 cctcccgccg ccgcttgggg aatcgttgcg ccacgtcggt gacgtcgtcg aggtagagat
     2821 cgtgcagcac gacgagcgtg ccgtcgcgcg ttgcgacgac gtcttgttcc aaaaaatcgg
     2881 cgccgaggcc gtgagccagc gccttcgctg cgagcgtgtg ctccggcaga tacccgctag
     2941 cgccgcgatg ggcgatgacg atcggcgccg gcgtcatggc actgcctgcc gatcagagct
     3001 tgaactcgat cgtttcccaa cgctgcgtgt cttcgtgccg cgcctgccgc cgcacggcat
     3061 cgatgatcaa ccgctcgttc cagccgatga cctcggccgc tcgtttcgcg acttccgccg
     3121 gaaccttcac gttgactccg aagggctcca tcgcgacgag cggcaggttg ctcgcctgag
     3181 ccgcgtccat ctgataggtg gcccaatact cgttctcgat gtacagagag ctcaagacga
     3241 cgacgacctc ggccgtcttc atctgcgtgc gatattcctc tttgagcgct tccttgccgc
     3301 ccgttgccgg taagtgatcg ggcacgctgc agttccgata gaaaaagttg ggcagagctt
     3361 cgaggtattc gaacacgcgg tggtagtcgg gatggctggc gaacgcatgc gtgacgtaga
     3421 tcctgatcgg atttttcgca gacattgctc tgttccgaga cgagtgcttt ggacgctact
     3481 actgctgaat cgcgtttgca acgaacggcc tcgccgattg cgtgacgtcg cacacacggg
     3541 gccgggaacg atttctcgat acatgccgac gcaccgcgtc cggtaaacta gtaacgacga
     3601 tgcgattact gctctacaac attcgctacg gtttgggttt gggcacggcg ctgcactggc
     3661 cgctgccggg cgtcggctat ctactcggta accgcaccaa tttgcagcgg atcaccgact
     3721 ttataaaatc gcaggatccg gatgtcgtcg gcctcatcga ggtcgacacc ggttccatca
     3781 gaactcgcag cgtcaaccaa gctgacgtga tcgcgcgcgc actgggccac tattccgtcg
     3841 accagtgcaa gtacggcgcg aactcgctga atcagcgctt accgatcttg cgcaagcaag
     3901 gcaatgcgtt cttggcggcg ccgcgagtcc acggcgagcg gtttcactat ttcgacaaag
     3961 gcatcaagcg cctcatcatc gagctcgagc tcgaaggctg cg
//
LOCUS       DATLEE010000341         3566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7645475, whole genome shotgun sequence.
ACCESSION   DATLEE010000341 DATLEE010000000
VERSION     DATLEE010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7645475"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VM692_07315"
     CDS             complement(<1..470)
                     /locus_tag="VM692_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ52014.1"
                     /translation="MLFVGVLLGALDIAIVGPALPAIRDALAVEPRWLPAVFSIYVLL
                     YVIGTPLLAKRSDRHGRRRVFVESLALFGAGSMIAAAAWSLPVLLTGRAIQAFGAAGL
                     LPVAAAVIAETVPLERRGRTLGLIGAVFGVAFLLGPLLGGLLLRSSWRWLFLVN"
     gene            complement(503..1033)
                     /locus_tag="VM692_07320"
     CDS             complement(503..1033)
                     /locus_tag="VM692_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUQ52015.1"
                     /translation="MRASLLAVGARRFAQRGIEPVSVEELIAEADVSRATFYGLFSSK
                     YSLLEGILNPVFELATRRVRELCARPAVEAVDGLVDVYWSLWTTHRDGLLLIPLVDQE
                     TFKHFEPQHRALQDALLELLLAAERGDLLRNGSAQYSLKVIARTAVQLLRVYDGHAAA
                     AALFRDAVRGLLLRAH"
     gene            complement(1143..2114)
                     /gene="dmeF"
                     /locus_tag="VM692_07325"
     CDS             complement(1143..2114)
                     /gene="dmeF"
                     /locus_tag="VM692_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HUQ52016.1"
                     /translation="MQEDALKRWRHDHSFGQDRRRPGERKTHLVIAITAAMMVVEIAA
                     GVLFGSMALLADGLHMASHAVALGINAYAYAYARRHAHDASFSFGTGKVNTLGGFTGA
                     ILLAAFALLMASESVQRLVVPVAIAFDQAILVAVVGLVVNGVSMLILGHRHANEHDHD
                     DHDEHDHGLHDHEGHAHEHHEHADHNLLSAYLHVLADALTSLLAIVALLAAKYFGLTW
                     MDPLMGIVGALLVARWSLGLLRATSAVLLDRSAAPEVCAAIREGIERVDGNSVADLHV
                     WCIGLNLYAVELAVVAAEPQPPEHYKALLPPGLNIVHATVEVHARSR"
     gene            complement(2154..3338)
                     /locus_tag="VM692_07330"
     CDS             complement(2154..3338)
                     /locus_tag="VM692_07330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ52017.1"
                     /translation="MPRLWLKGGREKSLRRRHPWIFSGAIERVEGKPGVGATVDIVGT
                     QGEVLARGAYSPSSQIRARVWSFEAGEGIDGEFFRRRLTRAVASRRSLGLLDARTACR
                     LVFSESDGLPGLIVDRYGDYLVCQFLSAGADAWRATIVELLAALCTPHGIYERSEGGA
                     RHKEGLPSRRELLAGDEPPRELEVVAGGARFFVDIANGQKTGAYLDQQRNRERVAAHA
                     RDAEVLDAFSYTGGFSVACLLGGAKSATLVDSSAEALVLAERETSANAVRERCRFVTA
                     NVFDELRALRQQGTRFDVVVLDPPKFVHSADQVNAGSRGYKDINMLGLALVRPGGVLA
                     TFSCSGHVDAALFQKIVAGAAVDADRAAQILERLAQPPDHPVATEFPEGDYLKGLILR
                     VH"
     gene            3433..>3566
                     /locus_tag="VM692_07335"
     CDS             3433..>3566
                     /locus_tag="VM692_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ52018.1"
                     /translation="MDLGLKGKNAAITGSSQGIGYAIATALAKEGCNVALSARGEERL
                     "
BASE COUNT          681 a         1211 c         1149 g          525 t
ORIGIN      
        1 atgttgacga ggaacaacca acgccaactc gagcgcagca gcaatcctcc gagtagcggc
       61 cccagcaaga acgcgacgcc gaacacggcg ccgatgagcc cgagcgtgcg gccgcggcgc
      121 tcgagcggca cggtttccgc gatgacggcg gccgcgacag gtagcagtcc cgcggcgccg
      181 aacgcttgaa tcgcgcgccc cgtcagcagc accggcaacg accacgccgc ggcggcgatc
      241 atcgagccgg cgccgaacag cgcgaggctc tcgacgaaca cccggcgccg gccatgccga
      301 tccgagcgct tcgcgagcag cggcgtgccg atcacgtata gcaacacgta gatgctgaaa
      361 acggccggca gccaccgcgg ctcgacggca agggcgtcgc gaatcgccgg caaggccggc
      421 ccgacgatcg cgatgtcgag cgcgcccagc aacacaccga cgaacagcac ggccaaggtg
      481 gccctctcgc gcgcgaccgt cactagtgcg ctcgcagcag caggccgcgg acggcatcgc
      541 ggaacagagc cgccgcggca gcatgcccgt cgtagacgcg gagcagttgg acagccgttc
      601 gggcaataac cttcaacgag tactgcgcac tgccgttgcg cagcaggtcg ccacgctcgg
      661 cggccagcag caactcgagc agagcgtcct gcagcgctcg atgctggggc tcgaagtgct
      721 tgaaggtttc ctggtcgacg agcggaatca gcagcaagcc gtcgcgatgc gtcgtccaaa
      781 gagaccaata gacgtcgacg aggccgtcga cggcttctac ggcgggtcgc gcgcacagct
      841 cgcggacacg ccgtgtcgct agttcgaaga cgggattaag tatcccttcc aaaaggctgt
      901 atttgctaga gaacaaaccg tagaacgtcg cgcgcgagac atcggcctcc gcgatcagct
      961 cctcgacgct gaccggctct atcccacgct gcgcgaagcg ccgggcgccg accgcaagca
     1021 acgaggcccg catccgctcg cggcggcgct gcactctggc gctggtggaa gtgctcggcg
     1081 agggcactgg cggccgcgat ttcacgccga aatcatacag cagtatctgt tctcgaacgg
     1141 ccttatctgg atctcgcatg gacctcgacc gtggcgtgca cgatgttcag ccccggcggc
     1201 agcagcgctt tgtaatgctc cggcggctgc ggctcggcgg cgacgacggc cagctccacc
     1261 gcgtacagat tcaacccgat gcaccagacg tgcagatcgg cgacgctgtt gccgtcgacg
     1321 cgctctatcc cttcgcggat cgccgcgcag acctcgggcg ccgcgctgcg atcgagcagc
     1381 acggcgctcg tggcgcgcaa caacccgagc gaccagcgcg caacgagcaa tgcgccgacg
     1441 atgcccatta ggggatccat ccaagtcaag ccgaagtact tcgcggccag caacgcgacg
     1501 atcgcgagca acgaagtcag cgcgtcggcg agcacgtgca gataggccga cagcagattg
     1561 tgatcggcgt gctcgtgatg ctcgtgcgcg tgaccttcat gatcgtggag gccgtgatcg
     1621 tgctcgtcgt gatcgtcatg gtcgtgctcg ttggcgtggc gatggccaag aatcagcatc
     1681 gagacgccgt tgacgacgag ccccacgacc gcgaccagga tcgcctgatc gaacgcgata
     1741 gccaccggca cgacgagccg ttgcacgctc tcggatgcca tcaacagcgc aaacgcggcg
     1801 agcaggatcg cgcccgtgaa gccgccgagc gtgttgacct tgcctgtacc gaaactaaat
     1861 gaggcgtcgt gcgcgtgccg gcgagcataa gcgtacgcgt acgcattgat gccgagcgcc
     1921 acggcgtgcg acgccatgtg cagaccgtcc gcgagcagcg ccatcgagcc gaacagcacg
     1981 ccggctgcga tctcgacgac catcatcgcc gccgtgatcg cgatcacgag gtgagtcttg
     2041 cgctcgccgg gacggcggcg gtcctggccg aacgagtgat cgtggcgcca acgtttcaag
     2101 gcgtcttctt gcatcgagcg gctcgagcta cagggaatta cggcattgca gcgtcagtgg
     2161 acgcgcaaga tcaagccttt gaggtaatcg ccctcgggga attccgtcgc gaccggatga
     2221 tcgggcggtt gcgcgagtcg ctcgaggatt tgcgcggcgc ggtccgcatc gacggccgct
     2281 ccggcgacga tcttctggaa cagcgcggca tcgacgtggc ccgagcacga gaacgtcgcc
     2341 agcactccgc ccggccgcac gagggcgagg cccagcatgt tgatgtcctt gtaaccgcgg
     2401 ctgccggcgt tcacctggtc ggcagagtgg acgaacttcg gcgggtcgag cacgacgacg
     2461 tcgaaacgcg taccttgctg acgcaacgcg cgcagctcgt cgaaaacgtt cgccgtgacg
     2521 aaccggcacc gctcgcgaac ggcattggca ctcgtctcgc gctccgccag cacgagcgct
     2581 tcggccgacg agtcgacgag cgtcgcgctc ttggcaccgc cgagcagaca cgccaccgag
     2641 aacccacccg tgtacgagaa cgcgtcgagg acctcggcgt cgcgcgcatg ggctgcgacg
     2701 cgctcgcggt tgcgttgctg gtcgagatac gcgccggttt tctgaccgtt cgcgatgtcg
     2761 acgaaaaacc gcgcgccgcc cgcgacgacc tcgagctcgc gcggcggctc gtcgccggcc
     2821 aacagctcgc gtcgcgacgg caagccttcc ttgtgacgcg cgccgccctc cgagcgttcg
     2881 taaatgccgt gcggcgtgca gagcgcggcc agcagctcga cgatggtcgc gcgccacgcg
     2941 tcggctccgg ccgacagaaa ctggcagacg agatagtcgc cgtagcgatc gacgatgagt
     3001 cccggcagtc cgtccgattc cgagaacacg agtcggcacg ccgttcgcgc gtcgagtaac
     3061 ccgaggctgc gccgtgatgc aaccgcgcga gtcaggcggc ggcgaaaaaa ctcgccgtcg
     3121 atgccctctc ctgcttcaaa gctccaaaca cgcgcgcgaa tttgcgacga cggtgaatag
     3181 gcgccgcgcg cgagcacttc gccttgcgtc cccacgatat cgacagtcgc gccgacgccg
     3241 ggcttaccct cgacgcgctc gatcgcaccc gagaatatcc aaggatgccg gcgccgcagc
     3301 gacttctctc gccccccttt gagccaaagc cttggcagct cagacgccat cgaacgatcc
     3361 tcaccgatcc ctattgtcga gtgcgcgcat tctataatcg ccccccacca ctgtcgtcag
     3421 cggagagtgc tcatggatct cggactcaaa ggcaagaacg cggccatcac cggctctagt
     3481 caaggcatcg gctacgcgat cgcgacggcg ctcgccaagg aaggctgcaa cgtggctttg
     3541 tcggctcgcg gcgaagagcg cctgaa
//
LOCUS       DATLEE010000342         3808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4859341, whole genome shotgun sequence.
ACCESSION   DATLEE010000342 DATLEE010000000
VERSION     DATLEE010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4859341"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="VM692_07340"
     CDS             complement(<1..1132)
                     /locus_tag="VM692_07340"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HUQ52019.1"
                     /translation="MRAPIALSKTKLLSLTQCRRKLWLETYSPELVEEPSAEKTALLE
                     TGNAVGEIARRLYGGSGGGHLVSFERGLRAAIDSTRALIAAGGSEPIFEATFDHLGVS
                     ARIDVLDRSEPRPKLIEVKSSARVKEHHLDDCAIQAWALTENGLAPRQIAVATLNTDF
                     VYAGDARYDGLLVETDVTELVQPLVADVAQLVTEARRTLADLDEPAVPVGMQCGAPHT
                     CEFYEHCAPPAGEYPVLGLGGSREKLFELLHAGYSDLRDVPESELQNDVQRRIWQQSR
                     LRVPYVGAELRELVASLPFPRYYLDFETIGPAVPIFAGTRPFEALPFQWSCHVEKSKR
                     SIEHAEFLELKAEPPMRRLAESLLATLGTRGPILVYTPYERQV"
     gene            1259..2485
                     /locus_tag="VM692_07345"
     CDS             1259..2485
                     /locus_tag="VM692_07345"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ52020.1"
                     /translation="MTRQAVSLGVGLALGLALAADAVAQPPPGPQVNLPKAGAPLTLQ
                     AGRQKVRVALVADGVAGPWDLVFIPGTSDLLVSESNGKLRMVKGGKLLPDIVWTPPSP
                     SGNDILHGVVVHPDFATNKLVYVSYLKGDNERQTLGISRGRLDGTKLVDAKEIFVADA
                     WENARPAFTGRMEFGPDKTLYVSVGDRDRLCCGRQDDNSIRIRAQSLDNDVGKILRLK
                     EDGSVPADNPFVGRANAKPEIFTYGHRNSYGLRFHPVTGELWQMEIGPNGGDEVNILK
                     PGANYGWPLVSLGRNYSGTLVSDKPFHRDGMEDPVIFWTPQISPAAMAFYTGDKFPQW
                     QNSLIIGALSGQHVERMPFNEQGQPLRREEFLSELGMRFRDVEIGGDGYIYFATEVRY
                     GSGQPDGAIIRLEPAE"
     gene            2511..3230
                     /locus_tag="VM692_07350"
     CDS             2511..3230
                     /locus_tag="VM692_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUQ52021.1"
                     /translation="MKPFTRVLAFALLATATSSAFAAKIAVIGTGNVGAALGPEFAAL
                     GHTIVYGSRTPNEQDVKDLVAKTGRGATATTQPEAVKGADMVLLAVPGNLAEQITKSL
                     GDLSGKIIIDPTNRVNRSSADGYANHDVPGSSNAELIQKAAPGAKVVKAFNTLNWTKM
                     VDPQSSGGPVSIPIVGDDAAARAAVAELVKGMGLEPVDLGPLRFANTLEEMLVIWANA
                     RSHGAAYNYYLRPEPAAAPAR"
     gene            3342..>3808
                     /locus_tag="VM692_07355"
     CDS             3342..>3808
                     /locus_tag="VM692_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52022.1"
                     /translation="MAAVLCGFLCGTGFAQELDVNGYSKVVVPRSQGDYFNYQAGLRE
                     AARDAGFELNRSVEEIPSEDWPKTLYLRAGLNLKTIGIYVVVYDVALQTPIAVCQWRL
                     VADRRVSPARNFDKRIAAILRALIDDMGYRGFDQSAYEANVRARNSVASANQQS"
BASE COUNT          672 a         1226 c         1278 g          632 t
ORIGIN      
        1 ggacctgccg ctcgtacggc gtgtagacga ggatcggccc acgcgtgccg agcgtcgcga
       61 gcaggctctc ggcgagccgg cgcatcggcg gctccgcttt gagctcgaga aactccgcgt
      121 gctcgatcga gcgtttgctc ttctcgacgt ggcaggacca ctggaatggc agggcttcga
      181 acgggcgcgt gcccgcgaag atcggcacgg ccggcccgat cgtttcgaag tcgaggtagt
      241 agcgcgggaa cggcagcgag gcgacgagct cgcgcagctc cgcgccgacg tacggcacgc
      301 gcagtcgcga ctgctgccag atgcgccgct gcacgtcgtt ctgaagctcc gactccggca
      361 cgtcacgcaa gtcgctgtaa ccggcatgca gcagctcgaa caatttttcc ctgctgccgc
      421 ccaagccgag caccggatat tcgccggccg gcggcgcgca gtgctcatag aactcgcacg
      481 tgtgcggtgc gccgcactgc atgccgacgg gaaccgcggg ctcgtcgaga tcggccagcg
      541 tgcgtcgcgc ttcggtcacg agctgcgcga cgtcggcgac cagaggctgc acgagctccg
      601 tgacgtcggt ctcgacgagc aagccgtcgt agcgcgcgtc gccggcgtag acgaagtcgg
      661 tgttcaacgt cgcgacggcg atctgccgcg gcgccaagcc gttttcggtc agcgcccacg
      721 cttggatcgc gcagtcgtcg agatggtgct ccttgacgcg cgccgacgat ttgacctcga
      781 tgagcttggg ccgcggctcg ctgcggtcga gcacgtcgat gcgcgcgctg acgcccaagt
      841 gatcgaacgt cgcttcgaag atcggctcgc tcccaccggc ggcaatcagc gcgcgcgtcg
      901 agtcgatggc tgcgcgcaac ccgcgctcga agctgaccag atggccgccg ccgctgccgc
      961 cgtagagccg ccgcgcgatc tcgccgacgg cgttgcccgt ctcgagcagc gcagtcttct
     1021 cggccgacgg ctcctcgacg agctcagggc tgtacgtctc gagccacagc ttgcgccggc
     1081 attgcgtcag cgagagcagc ttcgttttcg agagggcgat cggcgcgcgc atggcggcgc
     1141 agcatgcccg cggcggcgca gcggcacaac cgctgcggat ttctccggat gacagcgggg
     1201 acggcgggac ggtagcatcg gctgcaatcc gtataacaag aactcgtggg gggatttcat
     1261 gacacggcag gcggtttcgc tcggcgtcgg cttggctttg ggtttggcgc ttgccgccga
     1321 cgctgttgcg caaccgccgc cggggccgca ggtgaacttg ccgaaggccg gcgcgccgct
     1381 gacgttgcaa gctggccgac aaaaggtccg cgtcgcgctc gtggctgacg gcgtcgcggg
     1441 accatgggat ctcgtcttca ttcccggcac cagcgatctg ctcgtcagcg agagcaacgg
     1501 caagctgcgc atggtcaagg gcggcaagct gctgccggac atcgtctgga cgccgccgtc
     1561 gccgagcggc aacgacatcc tgcacggcgt cgtcgtgcat cccgacttcg cgacgaacaa
     1621 gctcgtctac gtgtcgtatc tgaagggcga caacgagcgc cagacgctcg gcatctcacg
     1681 cggtcggctc gacggcacca agctcgtcga cgcgaaagag atcttcgtcg ccgatgcgtg
     1741 ggagaacgcc cggccggcgt tcacgggccg catggagttc gggcccgaca agacgctgta
     1801 tgtcagcgtc ggcgatcgcg accgactgtg ctgcggccgc caggacgaca acagcatccg
     1861 gattcgcgca cagagcttgg acaacgacgt tggcaagatc ctgcgcttga aggaggacgg
     1921 cagcgtgccc gcggacaatc cgttcgtcgg tcgcgcgaat gcgaagcctg agatcttcac
     1981 gtacggccat cgcaacagct acggcttgcg ctttcatccc gtgaccggcg agctgtggca
     2041 gatggagatc gggccgaatg gcggcgacga ggtgaacatt ctgaagcccg gcgccaacta
     2101 tgggtggcct ctggtttcgc tggggcgcaa ctactcgggc acgctggtgt cggataaacc
     2161 tttccatcgc gacggcatgg aagaccccgt catcttctgg acgccgcaaa tcagtcctgc
     2221 ggccatggcg ttctacacgg gcgacaaatt tccgcagtgg cagaacagtt tgatcatcgg
     2281 cgcgttgagt ggccagcacg tcgagcgcat gccgttcaac gagcagggtc aaccgctgcg
     2341 ccgcgaggaa ttcttgagcg agctcggaat gcggttccgc gacgtcgaga tcggcgggga
     2401 cggctacatc tatttcgcca ccgaagtccg ctacggcagc ggccagcccg acggcgctat
     2461 catccgcctc gagcccgccg aatagctggg ccgctacaca ggagaacagc atgaaaccgt
     2521 tcacacgagt actcgcgttc gcgcttctcg ccacggcgac gagctctgcg ttcgctgcaa
     2581 agatcgccgt catcgggacg ggcaacgtcg gcgcggcgct cggccccgag ttcgcggcgc
     2641 tcggccacac gatcgtgtac ggctcgcgca cgccgaacga gcaggacgtc aaagacctcg
     2701 tcgcgaagac ggggcgtggc gcgacggcta cgacgcaacc ggaggccgtc aaaggcgccg
     2761 acatggtgct gctggccgtg ccaggcaact tggccgagca gatcacgaaa agcttgggcg
     2821 acctgtcggg caagatcatc atcgacccga cgaaccgcgt gaaccgcagc tcggccgacg
     2881 gctacgcgaa ccacgacgtg ccgggcagca gcaacgccga gctgatccag aaggccgcac
     2941 cgggcgcgaa ggtcgtcaag gcgttcaaca ccttgaactg gacgaagatg gtcgatccgc
     3001 agagctcggg cggcccggtt tcgattccga tcgtcggcga cgacgcagcc gcgcgcgcag
     3061 ccgtggccga gctcgtcaaa ggtatggggc tcgagcccgt cgacttgggt ccgctgcgct
     3121 tcgcgaacac gctcgaagag atgctcgtga tctgggcgaa cgcccgcagc catggcgccg
     3181 cgtataacta ctatctgcgt ccggagccgg cggcggcgcc cgcgcgctga ccgaggtagg
     3241 cagtgcccca ctcccgggcg atcctcggcg agtgtcggtt ggcttcgagc cctccgtgga
     3301 ggggcgccgg cgccctcccg tggggtattg gcagactgcg cttggctgcg gtcctttgtg
     3361 gttttctttg tgggaccggc ttcgcacagg aactcgacgt taacggatac tcaaaggtcg
     3421 tggtccctcg ttcgcagggc gactatttca actaccaggc cggccttcgc gaggcggccc
     3481 gtgacgcggg cttcgagctg aataggagcg tcgaagagat tccttcagag gactggccca
     3541 agaccctgta cctgagggcc gggctgaacc tcaaaaccat cgggatatat gtcgtggtgt
     3601 acgacgtcgc cctgcaaact ccaattgcag tgtgccaatg gcgattggtc gctgaccgtc
     3661 gcgtgtcacc ggcgcgtaat ttcgataaaa ggatcgccgc gattctcaga gcactcatcg
     3721 acgacatggg atatcgcggg ttcgaccaga gcgcgtacga agccaatgtg cgtgctcgga
     3781 attccgtggc cagcgccaac caacagtc
//
LOCUS       DATLEE010000343         4982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_392716, whole genome shotgun sequence.
ACCESSION   DATLEE010000343 DATLEE010000000
VERSION     DATLEE010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392716"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VM692_07360"
     CDS             complement(<1..227)
                     /locus_tag="VM692_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52023.1"
                     /translation="MTTKLAIAFALTFVAAAAAAQQSGLSQRRAPLANVDDLEPAVGN
                     ASTGTTGGSSAGSDFGARTYGGSESASQIRL"
     gene            complement(224..1069)
                     /locus_tag="VM692_07365"
     CDS             complement(224..1069)
                     /locus_tag="VM692_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ52024.1"
                     /translation="MSKPFVDLVLLQQRLRTAVSGALLLTLAACGSSTPRQADVPPPR
                     EVVAPAAPVAVGRSQSDSRRERRAAEQDTETGRAAPVAAEPERRERRGERRAAEEAAE
                     AAASGVVEPVPEVAAAAYQRALSAMLAENWLEAELELEQLALEQPSYPGPHVNLALVY
                     LHDGRRDDARAALDRALELEPGHAQANTQLGIMLREDGKFVEAEAAYRRALATDPEHA
                     LAHYNLGVLLDIYLRRTAEALEHYEHYQSSLAQPNETVARWIIDLRRRVGNGEAARVA
                     RAGGQ"
     gene            complement(1050..3875)
                     /locus_tag="VM692_07370"
     CDS             complement(1050..3875)
                     /locus_tag="VM692_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ52025.1"
                     /translation="MTNAPLLVLLTISAIWPFGRDRDREKPVEPTGTIKDLERAKIEV
                     DTSAAIVGSEAKAIESYRLFLDVASDDPLLKAEAMRRLADLQLETADVEELASNVESL
                     GTLGSTIDMYERLLESYPKYAKNDLVLYQLARAYEAEGRVDDSLATLDRLIAQYPQTA
                     HLAEAQFRRGETLFVQKRYRDAERAYAHVLEEGDASAFYEQSLYKHGWSLFKQQLYED
                     SLTSFFGLLDRQLGTDNDASGDRDPAALYAAMGRAQQELVDDTFRVLSIGFSYLDGPE
                     SISRYFNRRGARPYAFIAYTNLGDLYLEQERYQDAADAYHAFVELDPYHAKAPLLQVE
                     VIEAFKKGSFADLVLDGKENFVETYGPGSPYWQRFTFEQQPEVVAHLKSNVTDLAAYH
                     HAEAQKTKESREYAAAARWYRTYLQSFPDDANAAQTNFLLGEVLFESGNFRDAASEYE
                     RTAYAYPFHEKGGEAGYAALLAYAKHEEELRGSERADWHRRGIDSALKFADTYPTHAQ
                     AATVQTDAAEKLFALGEFGRARDVGRLVVARTPAAEPALLRTAWTVVAHSEFDLQDFA
                     AAETAYVQLVNFVPGDDPQRAEITERIASSIYKQGEQARSAGQLDVAVDHFLRVSQAA
                     PSSPVRATAEYDAGAALIQMGDWQRATTVLENFRTAFPNHQLAADVTTKLAVAYVEAG
                     NSARAAGEFERIADGDGPVEVRREALWRSAELYETTGQSVAATAAFGRFVERYPQPLA
                     EAIEARQKLIDLAGAAGNQAERARWMRDLVAADAMAGAERTDRSRYLAAKSQLELATP
                     TRDAFLGTRLVVPLDKSLAAKQTRMQEALAAYNKAAEYGIAEVTTAANYEIAELYHAL
                     SKDLYASERPPELTTEELEQYDILLEEQAFPFEEEAIQLHERNASRTTDGIYDEWVRK
                     SLTALVELQPGRYAKAEIGETLVEAIR"
     gene            complement(3872..>4982)
                     /locus_tag="VM692_07375"
     CDS             complement(3872..>4982)
                     /locus_tag="VM692_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52026.1"
                     /translation="DYRAALAPWLALRERSLLDSAVQESLLAVPYAFAQLGADKQAAD
                     HYVDAIEAFSSEIVRLTSSIESIENGELITRLLGEREDVANEASGWYWRLEHIPDSVE
                     SRYLYELLASNRFQEGLKNYRDLLQLNRNLDRWTESLGAFDDILDTRQRAYVQRLPKI
                     DSSLSSVDLDAMTARRVELESRLNAIERTEDVVALGTAKEQETWARLTAMEPKLALLP
                     NDAASEEMRAKHRFLRGLALWDLQRDYKARLWAERKSLTELDRSLREAQRRDHQVASS
                     RDDWPEKFSSLTARIEGLRPRVLGLRESAQVALLRQQQFLQGIAVEELKAQRDRLNTY
                     MVQARFALASIYDRAAAHVEPAGGNQAAALAGSGQ"
BASE COUNT          717 a         1690 c         1690 g          885 t
ORIGIN      
        1 tcgagccgga tctgcgacgc gctctcggag ccgccgtacg tccgcgcgcc gaagtcggaa
       61 ccggcgctgc ttccgcccgt cgtgccggtt gacgcgtttc cgaccgcggg ctcgagatcg
      121 tcgacgttcg cgagcggcgc gcgccgctgc gataagccgc tctgctgcgc cgcggccgcc
      181 gctgcgacga acgtcagcgc gaatgcgatc gcgagcttcg tcgtcattgt ccgcccgccc
      241 gagcgactcg cgcggcctcg ccgttaccga cgcggcggcg cagatcgatg atccagcgcg
      301 ccaccgtctc gttcggctgc gcgagcgagc tctgatagtg ctcgtagtgc tccagcgcct
      361 cggcggtgcg gcgcagatag atgtcgagca gcacacctag gttgtagtgc gcgagcgcgt
      421 gctcggggtc cgttgcgagc gcgcgccggt aggcggcttc ggcttcaacg aacttgccgt
      481 cctcgcgcag catgatgccg agctgcgtat ttgcctgcgc gtgacccggc tcgagctcga
      541 gcgcacggtc gagcgcggcg cgcgcgtcgt cgcggcgccc gtcgtgcaga tatacgagcg
      601 cgagattgac gtgcgggccg gggtaggacg gctgctcgag cgcgagctgc tcgagctcga
      661 gctcggcctc gagccaattc tcggcgagca tcgcactcag cgcgcgttga taagccgcgg
      721 ctgcaacctc gggcaccggc tcgacgacgc cgctggcggc ggcctccgcg gcttcctcgg
      781 ccgctcgccg ctcgccccgc cgttcgcgac gttccggctc cgctgcgaca ggtgccgcgc
      841 gccccgtctc ggtgtcctgc tcggcggcgc gccgttcacg gcggctgtcg gactgcgagc
      901 gcccgacggc gacgggcgcc gccggcgcta cgacctcgcg cggcggtggg acgtcggcct
      961 gcctgggcgt cgacgagccg caggcggcga gcgtcagcag cagcgcgccg ctcacggcgg
     1021 ttcgcagccg ctgctgcagt agcacgagat caacgaatgg cttcgacaag ggtctctccg
     1081 atttcagcct tcgcgtaacg tcccggctgc agctcgacga gcgccgtcaa gctcttgcgc
     1141 acccactcgt cgtagatgcc gtcggtcgtg cgcgaggcgt tgcgctcgtg cagctggatg
     1201 gcttcctcct cgaacggaaa ggcctgctcc tcgagcaaga tgtcgtactg ctcgagctct
     1261 tcggtcgtta gctcgggcgg gcgttcggac gcatacaagt ctttgctgag cgcgtggtag
     1321 agctccgcga tttcgtagtt cgcggccgtc gtgacctcgg cgatgccgta ctcggccgcc
     1381 ttgttgtacg cggcgagcgc ttcctgcatg cgcgtctgct tggccgcgag gctcttgtcg
     1441 agcggcacga cgagccgcgt gcccaagaag gcgtcgcgcg tcggggtcgc gagctcgagc
     1501 tgcgatttcg ccgcgagata gcgcgagcga tccgtgcgtt ccgcgccggc catcgcgtcg
     1561 gccgcgacga gatcgcgcat ccagcgcgcg cgctcggcct ggttgcccgc ggcgcccgcg
     1621 aggtcgatca gcttctggcg cgcttcgatc gcttcggcaa gcggttgcgg atagcgctcg
     1681 acgaaacggc cgaaagccgc ggtggcggca acgctctgcc ccgtcgtttc gtagagctcg
     1741 gccgagcgcc acaacgcctc gcgccgcacc tcgaccggtc cgtcaccgtc ggcgatgcgc
     1801 tcgaactcgc cggccgcgcg cgcgctgttg cccgcttcga cgtaagcgac ggccagcttc
     1861 gtcgtcacgt cggccgcgag ctggtgattc gggaacgccg tgcggaagtt ttcgagcacg
     1921 gtcgtcgcgc gctgccagtc gcccatttga atcagcgccg cgccggcgtc gtactcggcc
     1981 gtcgcgcgca caggtgacga cggtgccgcc tgcgagacgc gcaagaagtg atcgacggcg
     2041 acgtcgagct ggccggccga gcgcgcctgt tcaccctgct tgtagatcga cgaggcgatg
     2101 cgctccgtga tctcggcgcg ctgcgggtcg tcgccgggca cgaaattcac gagctgcacg
     2161 taggccgtct cggcggccgc gaagtcctgc aggtcgaact ccgagtgtgc gacgaccgtc
     2221 cacgccgtgc gtagcaacgc gggctcggcg gcgggggtac gcgcgacgac taggcggccg
     2281 acgtcgcgcg cgcgaccgaa ctcgccgagt gcaaatagct tctcggcggc atccgtctgc
     2341 accgtcgcgg cttgcgcgtg cgtcggatac gtgtcggcga acttcagcgc gctgtcgatg
     2401 ccgcggcgat gccaatcggc cctctcgctg ccgcgcaatt cctcttcatg tttcgcgtag
     2461 gccagcaacg ccgcatagcc ggcctcgccg cccttctcgt ggaacggata ggcgtatgcc
     2521 gtccgctcgt actcggacgc ggcgtcgcgg aagttgccgc tctcgaacaa cacttcgccg
     2581 agcaggaagt tcgtttgcgc ggcattcgca tcgtcgggga acgactgcaa gtacgtgcgg
     2641 taccagcgcg cggcggccgc gtactcgcgc gactccttcg tcttctgcgc ctctgcgtga
     2701 tggtaggccg cgaggtccgt gacgttgctc ttcaagtgcg cgacgacttc gggctgctgc
     2761 tcgaacgtaa accgctgcca gtacgggctg ccgggcccgt aggtctcgac gaagttctct
     2821 ttgccgtcga gcacgaggtc ggcgaacgag cctttcttga aagcctcgat gacctcgacc
     2881 tgcagcagcg gcgccttggc gtgatacggg tcgagctcga cgaaggcgtg ataggcgtcg
     2941 gcggcgtcct ggtagcgctc ttgctcgaga tagaggtcgc cgaggttcgt ataggcgatg
     3001 aacgcgtatg gccgcgcacc gcggcgattg aaataccgcg aaatcgactc cgggccgtcg
     3061 agatacgaga agccgatcga cagcacgcgg aacgtgtcgt cgacgagctc ctgttgggcg
     3121 cgccccatcg cggcatagag cgcggcggga tcgcggtcgc cgctcgcgtc gttgtcggtg
     3181 ccgagctgcc ggtcgagcaa gccgaagaac gacgtcaggc tgtcctcgta gagctgctgc
     3241 ttgaacagcg accaaccatg cttgtacagc gactgctcgt agaacgccga cgcgtcgccc
     3301 tcttcgagca cgtgcgcgta ggcgcgctcg gcgtcgcgat agcgcttctg cacgaacagc
     3361 gtttcgccgc ggcggaactg cgcttcggcg agatgagccg tctgcggata ctgagcgatg
     3421 agtcgatcca gcgtcgccag cgagtcgtcg acgcggcctt cggcctcgta agcgcgcgcg
     3481 agctggtaga gcacgaggtc gttcttcgcg tacttcggat acgactcgag cagccgctcg
     3541 tacatgtcga tcgtgctgcc gagcgtgccc agcgattcga cgttgctcgc gagctcctcg
     3601 acgtcggccg tctcgagctg cagatccgcg agccggcgca tcgcctcggc tttcaacagc
     3661 ggatcgtcgg acgcgacgtc gaggaacagc cgatagctct cgatcgcctt cgcttcgctg
     3721 ccgacgatcg cggcgctcgt atcgacctcg attttcgcgc gctcgaggtc cttgatggtg
     3781 ccggtcggct cgacgggctt ctcgcggtcg cgatcgcggc cgaacggcca gatcgcggag
     3841 atcgtcagca gcacgagcag cggcgcgttg gtcactggcc gctcccggcg agcgccgcgg
     3901 cctgattgcc gccggccggc tccacgtgcg ccgccgcgcg atcgtagatc gaggcgagcg
     3961 cgaagcgcgc ttgcaccata taggtgttca atcggtcgcg ttgcgctttg agctcttcga
     4021 cggcgatgcc ctgcagaaat tgctgctgtc gcagcagcgc gacctgcgcc gactcgcgca
     4081 agccgagcac acgcggcctc aagccctcga tgcgcgccgt caggctcgag aacttctccg
     4141 gccagtcgtc gcgcgagctc gcgacctgat gatcgcgccg ctgcgcttcg cgcagcgatc
     4201 gatcgagctc ggtcaagctc ttgcgctcgg cccacagccg cgctttgtaa tcgcgctgca
     4261 gatcccagag cgcgagcccg cgcaggaagc gatgcttcgc gcgcatctcc tcgctcgcgg
     4321 catcgttcgg taacagcgcg agcttcggct ccattgccgt cagcctcgcc cacgtttcct
     4381 gctctttggc cgtgccgagc gccacgacgt cctcggtgcg ctcgatcgcg ttgagccgcg
     4441 actcgagctc gacgcgccgc gccgtcatgg cgtcgagatc gacgctcgat aggctcgagt
     4501 cgatcttcgg cagccgctgc acgtaggcgc gttgccgcgt atcgaggatg tcgtcgaagg
     4561 cgccaaggct ttcagtccaa cgatcgaggt tgcgattgag ctgtagcaga tcgcgatagt
     4621 tcttgagccc ttcttggaaa cggttgctcg cgagcagctc gtagagatag cggctctcga
     4681 cggagtccgg tatgtgctcg agacgccagt accagcctga agcttcgtta gcgacgtctt
     4741 cgcgctcgcc gagcagccgc gtgatcagct cgccgttctc gatgctctcg atcgagctcg
     4801 tcaagcgcac gatctcgctg ctgaaggcct cgatcgcgtc gacatagtgg tcggcggcct
     4861 gcttgtcggc gccgagctga gcaaacgcgt acggcacggc caacagcgac tcttgcacgg
     4921 ccgagtcgag caagctgcgc tcacgcagcg cgagccacgg tgccaacgcg gcgcggtagt
     4981 cc
//
LOCUS       DATLEE010000344         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8393719, whole genome shotgun sequence.
ACCESSION   DATLEE010000344 DATLEE010000000
VERSION     DATLEE010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8393719"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..984)
                     /locus_tag="VM692_07380"
     CDS             complement(70..984)
                     /locus_tag="VM692_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUQ52027.1"
                     /translation="MSDLHQFVRAADIKPTAAFPKHSTGYRRFTLSDRSMGAVHSGWG
                     SCELDAGGRVDLAIHSFEKSFFVTSGNPVLFLEGRGYRLSPGACGLIPVGMKHAWLGP
                     TTGTAKWVDMAAPCPKGENFADGTYFLGPAPSVAIEEYDIRDVSSRHLFKMGEDDIKV
                     DSVRTGLKKDAAKVSASMATALLVYSGISLKMLVDQRLDAALHTMFMVEYEPNANAHP
                     HDHPLEEAYFIMHGEVEAWADDKQYFMKPGDFLWAGVGCTHAFYNRSNTTVRWLETQS
                     PQPPARNSYRWSRDWEYVAQQLERKGVA"
     gene            complement(1069..1737)
                     /locus_tag="VM692_07385"
     CDS             complement(1069..1737)
                     /locus_tag="VM692_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52028.1"
                     /translation="MKRTSRILAVGLLAATVTTLAQAQRPEMSFFVTSVGLGKGGNLG
                     GIKGADAHCQALAQSAGAGTKTWRAYLSVTDLNGKGAINARDRIGRGPWHNAKGVRIA
                     IDVDDLHSDSANITADTAITEKGQKLPTEPNQHDILTGSTRDGRAWTYNLLDDLNLES
                     GAMTCSNYTSDAENQFVMLGHHDRAGTAPISPWNAAHPSTGCNNERLASTGSAGLLYC
                     FAID"
     gene            1837..>2403
                     /locus_tag="VM692_07390"
     CDS             1837..>2403
                     /locus_tag="VM692_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ52029.1"
                     /translation="MKRGGLLVAVLSCSALLISFTAGAQPGPLWQSTTYKGREIGKLT
                     GDVYYARSDDYVSAFMVTTDGIVLVEPIGPEFATWLKGELQRRFGVPVKYVIYSHSHA
                     DHASGAAVFKDTARIIGHEALLGLLALPPSSTQLPQALRAQDTNGNGRIEKAEAQAQV
                     GASFEFYDADGDGAGATILFAQVGAGTLL"
BASE COUNT          390 a          779 c          784 g          450 t
ORIGIN      
        1 cgatggagcg gccgagaccg cccgtgccgc cgatcacgac gacgctcttg ttattattct
       61 cgctgcccat taggcgactc ccttacgctc cagttgctgg gccacgtact cccagtcgcg
      121 cgaccagcgg tacgagttgc gcgccggcgg ctgcggcgat tgggtttcca gccaacgcac
      181 ggtcgtgttg ctgcggttgt agaacgcgtg tgtgcacccg actccggccc aaaggaagtc
      241 gccgggcttc atgaagtact gcttgtcgtc ggcccacgcc tcgacctcgc cgtgcatgat
      301 gaagtacgcc tcctcgagcg ggtggtcgtg cggatgcgcg ttcgcgttcg gctcgtactc
      361 gaccatgaac atcgtgtgca gcgccgcgtc gaggcgctgg tcgacgagca tcttcagcga
      421 gatgccgctg tagacgagca gggccgtcgc catgctggcc gagaccttcg cggcgtcctt
      481 cttcaggccc gtccggaccg agtcgacctt gatgtcgtcc tcgcccatct tgaacaggtg
      541 ccgcgacgag acgtcgcgga tgtcgtattc ctcgatcgcg acgctcggcg ccgggccgag
      601 gaaatacgtg ccgtcagcga aattttcgcc cttcgggcac ggtgccgcca tgtcgaccca
      661 cttcgccgtg ccggtcgtcg gcccgagcca ggcgtgcttc atgccgacgg gaatcaagcc
      721 gcaggctccg ggggacaggc ggtagccacg gccctcgagg aacaggacgg ggttgcccga
      781 cgtcacgaaa aagctctttt cgaacgagtg aatggccagg tcgacccgtc cacccgcgtc
      841 gagctcgcag gagccccaac ccgagtgcac cgctcccatg gagcgatccg acagcgtgaa
      901 ccggcgatag cccgttgagt gcttggggaa cgccgccgta ggcttgatgt cggcggcccg
      961 aacgaactga tgcaagtcgc tcatgacgac acacctccag tggcacgccc atagttggac
     1021 gctggccgcg tgttgtcaaa cgcggcggcc gcatgcgaag agctgacctc agtcgatcgc
     1081 gaagcagtag agcaaaccgg cgctgcccgt cgaggcgagg cgctcgttgt tgcagcccgt
     1141 cgacggatgc gcggcattcc acggcgagat cggcgcggtt ccggcgcggt cgtgatggcc
     1201 gagcatcacg aactggttct cggcatcgct cgtgtaattg ctgcaggtca tcgcgccact
     1261 ctcgagattc aagtcgtcga gcaggttgta ggtccaggcg cggccgtcgc gcgtcgagcc
     1321 cgtcaggatg tcgtgctgat tcggctctgt cggcagcttc tggcccttct ccgtgatcgc
     1381 ggtgtcggcc gtgatgttgg cgctgtccga atgcagatcg tcgacgtcga tcgcgatgcg
     1441 aactcccttg gcgttgtgcc aaggtccgcg gccgatgcga tcgcgcgcgt tgatcgctcc
     1501 tttgccgttc aagtcggtga cgctcagata cgcgcgccaa gtcttggtac cggcgccggc
     1561 gctttgcgcg agcgcttggc aatgcgcgtc ggcgcccttg attccgccga ggttgccgcc
     1621 cttgcccaac ccgacgctcg tcacgaagaa cgacatctcg ggtcgttgcg cctgcgcgag
     1681 tgtcgtgaca gtggccgcga gcaagcccac ggcgagaatg cgggacgttc ttttcattgt
     1741 gtctctctcg aattttaccg gcgggattgc ggtcaacggc gatactagta atacggtagg
     1801 atcggggcaa ccgatggggg tcgcagggag attcgcatga agcgcggcgg tttgctcgtt
     1861 gccgtactga gttgttcggc gctgctcatt tcattcacgg ccggtgcaca acccggcccg
     1921 ctgtggcaat cgacgacgta caagggacgc gagatcggca agctgaccgg cgacgtctac
     1981 tacgcgcgca gcgacgacta cgtatcggcg ttcatggtga cgacggacgg catcgtgctc
     2041 gtcgagccga tcggccccga gttcgcgact tggctcaagg gcgagctgca gcgccgcttc
     2101 ggtgtccccg tcaaatatgt catctacagc cactcgcacg ccgaccacgc gtccggtgcg
     2161 gcggtcttca aagacacggc tcgcatcatc ggtcacgagg ccttgctcgg cctgctcgcc
     2221 ctgccgccga gctcgacgca gctgccgcaa gccttgcgcg cgcaggatac caacggcaac
     2281 ggccgcatcg agaaagccga ggcgcaggcg caggtcggcg cgtcgttcga gttctacgac
     2341 gccgacggcg acggcgccgg agccaccatc ctgttcgccc aggtcggcgc cggcactctg
     2401 ctc
//
LOCUS       DATLEE010000345         2713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3188849, whole genome shotgun sequence.
ACCESSION   DATLEE010000345 DATLEE010000000
VERSION     DATLEE010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3188849"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VM692_07395"
     CDS             complement(<1..1036)
                     /locus_tag="VM692_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HUQ52030.1"
                     /translation="MARCAELLLTSGTGTFQDAFATLAPVLELDAYACFIAGEGEPAF
                     LVESARGMSDTAISGTTIAGIVAELARADQRTLVLDSLRQATEAPATLFRDLGFTSAV
                     IGALVVDGHVLGAVVLAARAKERFTADERSLLDTALCYVAAAYERRDLLRHRHNAEQR
                     KREFLTVLAHELRNPLAPVRNALEIMRTNDRAHSPVEHSAYAMIERQLQQIGRLADDL
                     LDANQVVLGHVELEKQRVLLDAIIERAASQSRPWIEAARHSLSVELPRDVIALDADPK
                     RLARVFSNLVNHAARRMDRGGRIVISAERRDSSALVFVRARGRTVSGEDLSGIFADAV
                     DHREVAEGQAQ"
     gene            complement(1134..1298)
                     /locus_tag="VM692_07400"
     CDS             complement(1134..1298)
                     /locus_tag="VM692_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HUQ52031.1"
                     /translation="MKYAALLFPLVSAAGLTLLSACNTVEGVGRDLERAGDKIQDQAD
                     DARDRRRRDD"
     gene            1433..>2713
                     /gene="egtB"
                     /locus_tag="VM692_07405"
     CDS             1433..>2713
                     /gene="egtB"
                     /locus_tag="VM692_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HUQ52032.1"
                     /translation="MTSKRLITASEPLPEPLVRRYLAVRALSVELAARLTVEDQVVQP
                     CLEASPTKWHLGHTSWFFEHFVLRKQLPSFRPVDERYERLFNSYYYTVGEMHPRAERG
                     VLSRPTVAQIQDYRSRIDDAMTKLLESRPDDPQLSAVVELGLNHEQQHQELLLTDAKQ
                     VLFANPLDVAYSDAAAPPRTPRTEPLAFAIEPARFAEIGADGTAFAFDNERPRHRVWL
                     ESHALANRPVTNGEYREFIRDGAYTKPELWLADGWARLRERGWNRPLCWAADLEREYT
                     LGGWRTLDEHAPVCHVSFYEAAAFAAWAGARLPTEAEWENAAGAQTPVHGNLLETGWL
                     HPAAAAALPSGGGRFLQLWGDVWEWCASAYRPYPRFRPLSGSLGEYNGKFMCNQWVVR
                     GGSCATPAGHVRASYRNFFYPHDRWQFLGFRLARD"
BASE COUNT          424 a          937 c          886 g          466 t
ORIGIN      
        1 cttgcgcctg accctccgcg acttcgcggt ggtcaaccgc gtccgcgaag atgccgctca
       61 aatcctcgcc ggagactgtg cgcccgcgcg cgcgcacgaa gaccagcgcg ctggagtcgc
      121 gccgctcggc agagatcacg atccggccgc ctcgatccat gcgccgcgcc gcatggttga
      181 cgagattcga aaacacgcgt gccagacgtt ttgggtccgc gtcgagcgcg atgacgtccc
      241 gcggcagctc gacgctcaga ctatgtcgcg cggcctcgat ccacggccgc gactggctcg
      301 ccgcgcgctc gatgatcgcg tcgagcaaca cgcgctgctt ctcgagctcg acatggccga
      361 gcacgacttg gttcgcatcg agcagatcgt cggcgagccg tccgatctgc tgcaattgcc
      421 tctctatcat cgcgtacgcc gaatgctcga ccggcgagtg cgcgcgatcg ttggtgcgca
      481 tgatctcgag cgcgttgcgg accggcgcca gtggattgcg cagctcgtgc gcgagcacag
      541 ttaggaactc acgcttgcgc tgctccgcgt tgtgccgatg gcgcagcagg tcgcggcgct
      601 cgtaggccgc tgcgacgtag cacagagcag tgtcgagcag actacgctcg tcggccgtaa
      661 aacgctcttt cgcacgtgcc gcgagcacga ccgcaccgag cacgtgcccg tcgacaacga
      721 gcgcgcctat cacggcgctc gtgaagccca aatcgcgaaa cagcgtcgcc ggcgcttcgg
      781 tcgcctgacg taacgaatcg agcacgagcg tgcgctggtc agcgcgcgcg agctcggcca
      841 cgatgccggc gatcgtcgtc ccggagatcg cggtgtcgga catgccgcgt gcgctttcga
      901 cgaggaaggc cggctcaccc tcgcctgcga tgaaacacgc gtaagcgtcg agctcgagca
      961 ccggcgcgag cgtcgcaaat gcgtcttgaa acgtgccggt gccgctcgtc agcaacagct
     1021 cggcgcagcg cgccaaggtc tcgagctcgc actgtgcgcc gtcggcgtcg acccgcccgt
     1081 ccgcgcccga tgtgtgtcgc gcgcccgcgt cgcttgaccg agaagcgccg ccgttagtcg
     1141 tcgcggcgtc ggcgatctcg ggcatcgtcc gcttggtctt ggatcttgtc gccggcccgc
     1201 tcgaggtcgc gaccaacgcc ttcgacggtg ttgcaggcgc tcagcagcgt gagtcctgcg
     1261 gcagacacca gggggaacag gagtgctgcg tatttcatct ccgtgaatcc tttttgcgga
     1321 tggccccgca cccggagtcg ttgcagaaac cgtgccagtg gattcggttg ccgtggcaca
     1381 acgattgcag ctccctcgtt tgattccttg cgggcggcgt cgaccgaaac tcatgacttc
     1441 gaaacgcctc ataaccgcct cggagccgct gcctgaaccg ctggtgcgcc gctatctcgc
     1501 ggtgcgcgca ctctcggtcg agctggccgc ccgcctcaca gtcgaggacc aggtcgtgca
     1561 gccgtgcctg gaggcgagcc cgacgaagtg gcacctcggc cacacgagct ggttcttcga
     1621 acactttgta ctgcgcaagc agctgccgag cttccgtccc gtcgacgagc gctacgagcg
     1681 cctgttcaac tcgtactact acaccgtcgg cgaaatgcat ccgcgtgccg agcgcggcgt
     1741 gttgagccgg cccacggtcg cgcaaattca ggattatcgt tcccgtatcg acgacgccat
     1801 gaccaagctg ctcgagtcgc gccccgacga tccgcaactc tcggccgtcg tcgagctggg
     1861 gctgaaccac gagcagcagc accaagagtt gctattgacc gatgccaagc aggttctctt
     1921 cgcgaacccg ctcgacgtcg catactcgga cgccgcggcg ccgccgcgta cgccccggac
     1981 cgagccgctc gcattcgcga tcgagccggc gcggttcgcc gagatcggcg cggacggcac
     2041 cgcgttcgct ttcgacaacg agcggccacg gcatcgtgtc tggctcgaga gccatgcgct
     2101 cgcgaaccgg cccgtcacca acggcgagta tcgcgagttc atccgcgacg gcgcgtatac
     2161 caagccggag ctgtggttgg cagacggctg ggctcgcctg cgcgagcgcg gctggaacag
     2221 gccgctttgt tgggccgccg atctcgagcg cgagtacacg cttggcggct ggcgcacgct
     2281 cgacgagcac gcgccggtct gccacgtcag cttttacgag gcagcggcct tcgccgcgtg
     2341 ggccggcgca cgattaccga cggaggccga gtgggagaac gccgcaggcg cgcagactcc
     2401 cgtgcacggc aatctgctcg agacgggctg gctgcatccg gccgcagccg ctgcgctgcc
     2461 gtccggcggc ggccgctttc tgcaactctg gggggacgtc tgggagtggt gcgcgtcggc
     2521 ctaccgtccg tatccgcgct ttcgcccgct ctcgggatcg ctcggcgagt acaacggcaa
     2581 gttcatgtgc aaccagtggg tcgtgcgcgg cggctcgtgc gcgactccgg ccggccacgt
     2641 gcgcgcgagc tatcgcaact ttttctatcc gcacgatcgc tggcaattcc tcggctttcg
     2701 cttggcgcgc gac
//
LOCUS       DATLEE010000346         6705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_394994, whole genome shotgun sequence.
ACCESSION   DATLEE010000346 DATLEE010000000
VERSION     DATLEE010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394994"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="VM692_07410"
     CDS             <1..1995
                     /locus_tag="VM692_07410"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ52033.1"
                     /translation="ADITSDGFDNVSDTLKTSPLLLERYLTVGLRVAAMAVGNTKLSP
                     RGTEYRPRLDLSQNHWIEGLPFGTRGGLVVDHYFPTDAEYEIRPELWRATGSTVRGVE
                     GFKTPFELQILLDGAVVHSAQFGGAEDDALSNRDIGSAVMQVAARLPVRVRVSAGLHR
                     VGVTFVAKSFALEQKLLDPDRADLPIGNDGYGWPIITRALVAGPYDSTGSGDTPTRRA
                     IFTCRPSDSVSDATCADEILGRLALRAYGRPLDDQDRATLRALYASGSDNGRNFEAGI
                     EVGLARILSGPELLLYHGVTEAAALAEGGDANQDDGAMLASRLALFLWNSIPDDALRE
                     AGMAGRLRSPEVLEAEVRRMLADPRADTLVTDFAEQWLQLRLNNAKAPDQRTFPTFDD
                     NLRSDMLRETELFLRSVLFEDASLLDLLNADYTFLNERLAEHYGIDGVFGDAFRRVTL
                     QDPNRRGLLGQGSILFQTSVATRTSPVFRGKWILTNFFNAPPPPPPADVPALEASIAD
                     STPHSVRERLEQHRKDPVCAGCHAIIDPTGLALENFDAIGRWRDTDGGQPIDPSATLP
                     GGIAVSGPSSLREALLRRPELFVSTFTQKLMVYALGRRLEAEDMPTVRRIVREAARDD
                     YRFSALVLGIVRSDQFQQKARFAPNATQTVADASSIQETN"
     gene            1995..3299
                     /locus_tag="VM692_07415"
     CDS             1995..3299
                     /locus_tag="VM692_07415"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ52034.1"
                     /translation="MFITKKHLSRRTVLRGAVGAAIGLPFLDAMVPAASAAPPAQLRF
                     GAVYVPNGIRPEIFTPPPSPKLELPPLLERFVPLRDHINVVTGLRAPDPFGHHVIGSL
                     WLSGGTARPTQAADVQAGQTIDQHIADAIGRDSPLRSLELGIEGINTSSNSCQFGWSC
                     LYLNTLSWQTPTMPLPNEINPQNAFERMFGDIGSAEERAERFKSKASILDSVISAASR
                     MSGQLAPQDRSTIDEYLTNIREVESRIQSSGQRAEAAGETPDAPAGIPGSYDEHVRVM
                     YDLIALAWRADITRVTTFMQGVEASSIGYPQIGVPESHHIVSHHGGVAASIAKYAKIN
                     AYHLSLFGDFVEKLRTTPDGDGSLLDHSLVMYGSGMGNGDKHDQNMVPIVLAGTAAGR
                     VKGGRHIATPNQTPLANLIGGLGQAAGLELGKLANATGIVEL"
     gene            3337..3987
                     /locus_tag="VM692_07420"
     CDS             3337..3987
                     /locus_tag="VM692_07420"
                     /inference="COORDINATES: protein motif:HMM:NF044762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUQ52035.1"
                     /translation="MRSQRRAEARPAPKLGGKRERTRKKILEAAFGLMGNERGLTVRI
                     EEICAAARISRGTFYNYFTSLEQLFEVLAIELSHELNGALVATWDETQSHAEGSNAAI
                     QHYLRYAQRDPAWAWAMVHLSAFGPTFGVEAWEACHRAIAKGIEAGEFDVPNATVGRD
                     LMTGTVLASVRTMLRSGSDTSQPRVVAYHLLRALGVPDSRARDVAERPLSEIVAPP"
     gene            complement(4077..5498)
                     /locus_tag="VM692_07425"
     CDS             complement(4077..5498)
                     /locus_tag="VM692_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ52036.1"
                     /translation="MTTTIPERLDRLPWSRWHWRIVTALGITWVLDGLEVTMVGSLGP
                     TLQRDGTLGLSAADVGWAGSAYVAGAVLGALFFGRLADKLGRKPLFLVTLSVYLVATL
                     LTAGTWNFWSFAFCRFLTGFGIGGEYAAINSAIDELIPARVRGLVDLAINGSFWLGAA
                     AGAGLSIVLLEPGRLPEDAGWRVAFAFGAILTVAILLVRRHLPESPRWLMTRGRLAEA
                     EGVVARIEAEIEREQGPCVECRTAFAIRLRSHVSWREIFTLLVTRYRRRAVLSFVLMG
                     SQAFFYNAIFFTYALVLTSFYGVAANDVGYYIFPFAVGNFLGPLLLGRLFDSVGRRRM
                     IAATYGIAGVGLLASGYAFANDWLDATTMTGCWSVLFFFASAAASSAYLTVSEVFPLE
                     MRAVAIAIFYAIGTGLGGFVGPALYGALIGTGDREAVFVGYAVGGLLMVGAAVVAVWL
                     GIDAERKPLETVAQPLSAAVEET"
     gene            complement(5700..6440)
                     /locus_tag="VM692_07430"
     CDS             complement(5700..6440)
                     /locus_tag="VM692_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52037.1"
                     /translation="MDTKKIRFVANRPWLTKDSASRPEPIVKTLPDWYRKADWFVPDP
                     ATGKPAEAQDGSGKMPTWKACPAVLDVMGTGYTYKTPCAIEFAEDAAGNIHAKVLDPE
                     YRNVLVDRDPMPQFQHPWGYHQKHFAWWADWAVELPDGYSALYTHPLNRFELPFLTTS
                     GIVDNDKVHLPGTMPFFWLKGARGVLPAGTPYAQIIPFKREHWQSEIDTSLDVEQMVA
                     KNEENAAKYRRPDGGVYQQQVWERRKYQ"
BASE COUNT         1169 a         2238 c         2198 g         1100 t
ORIGIN      
        1 gcggacatca ctagcgacgg cttcgacaac gtttccgaca cgttgaagac ttcgccgctg
       61 ctgctcgaac gctatctgac ggttggcttg cgcgtcgctg cgatggcggt gggcaacacc
      121 aagctctcgc ctcgcggcac ggagtatcgg ccgcggctcg atttatcgca gaaccactgg
      181 atcgaagggt tgccgttcgg cacgcgcggc gggctcgtcg tcgaccacta cttcccgacg
      241 gacgcggagt acgagatccg acccgagctt tggcgagcga ccggtagcac cgtgcgcgga
      301 gtggaaggct tcaagacacc gttcgagctg cagatcctgc tcgacggcgc cgtagtccat
      361 tccgcccagt tcggcggtgc ggaggacgac gcgctctcga accgcgacat cggcagtgcc
      421 gtgatgcaag tcgcggcgcg gcttcctgtg cgcgtgcgcg tcagcgccgg cctgcaccgc
      481 gtcggtgtca cgttcgtcgc gaagagcttc gcgctcgagc agaagctact cgatccggac
      541 cgagcggact tgccgatcgg caacgacggc tatggctggc cgatcatcac gcgcgccctc
      601 gtcgcggggc cgtacgactc tacggggtcc ggcgacacgc cgacgcgccg agcgatcttc
      661 acgtgccggc cgagtgactc cgtcagcgat gccacgtgcg ccgacgagat tctcggccgc
      721 ctcgcactgc gtgcctacgg ccggccgctc gacgatcagg atcgtgcaac cttgcgcgcg
      781 ctctacgcga gcggcagcga caacggccgc aacttcgaag ccggcatcga ggtcgggctc
      841 gcacgaatct tgagcggccc cgagctcctg ctgtaccacg gcgtgacgga agcggccgcg
      901 ctggccgaag gcggcgacgc caaccaagac gacggcgcca tgctcgcctc gcggctcgcg
      961 ctgtttctct ggaacagcat tcccgacgac gcgctgcgcg aggccggcat ggccgggcgg
     1021 ctccgctcgc cggaagtgtt ggaggccgag gtgcggcgga tgctcgcgga tcctcgcgcc
     1081 gacacgctcg tcacggactt tgccgagcaa tggctgcagc tgcggctcaa caatgcgaag
     1141 gcacccgacc agcgcacgtt cccgaccttt gacgacaacc tacgcagcga catgctgcgc
     1201 gagaccgagt tgttcctgcg ctccgtgttg ttcgaggacg cgagcctgct cgatctgctg
     1261 aatgccgact atacgttctt gaacgagcgg cttgcggagc actatggcat cgacggcgtg
     1321 ttcggcgatg cgttccgccg cgtgacgctg caggacccga atcgccgcgg cttgctgggg
     1381 cagggcagca tcctgttcca aacgtcggtc gcgacgcgca cgtcgccggt gttccgcggc
     1441 aagtggattt tgacgaactt cttcaacgcg ccgccgccgc cgccgcccgc cgacgtgccg
     1501 gcgctcgaag ccagcatcgc ggacagcacg ccgcacagcg tgcgcgagcg cctcgagcag
     1561 catcgcaagg accccgtgtg tgcgggctgc cacgcgatca tcgatcccac ggggctcgct
     1621 ctcgagaact tcgatgcgat cggtcgctgg cgcgacacgg atggcggcca gccgatcgat
     1681 ccgagcgcga cactgcccgg aggcatcgcc gtttcgggcc cgagcagctt gcgtgaggcg
     1741 ctgctgcgcc gccccgagct gttcgtgtcg acgtttacgc agaagctcat ggtgtacgcg
     1801 ctcggtcgcc gcctcgaggc cgaggacatg ccgacggtgc gtcgcatcgt gcgcgaagcc
     1861 gcgcgcgacg attatcgctt tagcgcgctc gtgctcggca tcgtgcgcag cgatcagttc
     1921 cagcagaagg cgcgtttcgc gccgaacgcg acgcagaccg ttgccgacgc gtcgtcgatc
     1981 caggagacga actaatgttc atcacgaaga agcatctttc gcgccgcacc gtcttgcgcg
     2041 gggccgtcgg tgccgcgatc ggcctgccgt ttctcgatgc gatggttccc gccgcgagcg
     2101 cagcgccgcc cgcgcagctg cggttcggcg ccgtctacgt gccgaacggc attcggccgg
     2161 agatctttac gccgccgccg tcaccgaagc tcgagttgcc gccgctgctc gagcgattcg
     2221 tgccgttgcg cgaccacatc aacgtcgtca cggggttgcg cgccccggat ccattcggcc
     2281 accacgtcat tgggtcgtta tggttgagcg gcggaacggc gcggcccacg caagccgccg
     2341 acgttcaagc cgggcagacc atcgatcaac acattgccga cgccatcggc cgcgactcgc
     2401 cgctgcgctc gctcgagctc ggcatcgagg gcatcaacac ctcgtcgaac tcgtgccagt
     2461 tcggttggag ctgtctctac ttgaacacgc tgtcgtggca aacgccgacg atgccgctgc
     2521 cgaacgaaat caacccgcag aatgccttcg agcgcatgtt cggcgacatc ggctcggccg
     2581 aagagcgcgc cgagcgcttc aagagcaaag cgagcatcct cgattccgtg atctcggcag
     2641 cgagccgcat gagcggccag ctcgcgccgc aggaccgctc gacgatcgac gagtacctga
     2701 ccaacatccg cgaagtcgag agccgcatcc agtcatcggg gcagcgggcc gaagccgccg
     2761 gcgaaactcc cgacgcgcct gccggcatcc cgggctcgta cgacgagcac gtccgcgtga
     2821 tgtacgacct catcgcgctc gcgtggcgtg ccgacatcac gcgcgtgacc acgttcatgc
     2881 aaggtgtcga agcgagcagc atcggctatc cgcagatcgg cgttcccgag tcgcatcaca
     2941 tcgtctcgca tcacggcggt gtcgctgcga gcatcgccaa gtacgcgaag atcaatgcgt
     3001 atcacctttc gttgttcggc gacttcgtcg agaagctgcg cacgactccc gacggcgacg
     3061 ggtcgttgct cgatcactcg ctcgtcatgt acgggtccgg catgggcaac ggcgacaagc
     3121 acgatcagaa catggtgccg atcgtgctcg cgggaacggc ggcgggccgc gtgaagggcg
     3181 gtcgccacat cgcgacgccg aaccagacgc cgttggcgaa cctcatcggc gggctcggac
     3241 aagccgcggg gctcgagctc ggcaagctgg ccaatgccac gggcatcgtg gagctctgaa
     3301 ggcgagcgta tggtggccgt cccgacaaga caggcagtgc gctcgcaacg acgcgcggaa
     3361 gcgcgaccgg ctccgaagct cggcggcaag cgcgagcgca cgcgcaagaa gatcctcgaa
     3421 gccgcgttcg ggctcatggg gaacgagcga ggcctcacgg tgcggatcga agagatttgc
     3481 gccgcggccc gtatctcgcg cggaacgttc tacaactact tcacgagcct cgagcagctg
     3541 ttcgaggtcc tcgcgatcga gctcagtcac gagttgaacg gcgcgctcgt cgcgacatgg
     3601 gacgagacgc agagccacgc ggaaggctcg aacgccgcga tccagcacta cttgcgctac
     3661 gcgcaacgcg atcccgcgtg ggcatgggcg atggtccact tgagcgcgtt cggaccgacc
     3721 ttcggtgtcg aggcgtggga agcgtgccac cgcgcgatcg cgaagggcat cgaagccggg
     3781 gagttcgacg tgccgaacgc aaccgtcggc cgcgacctca tgacgggcac cgttctcgcg
     3841 agcgtgcgca cgatgctgcg ctccggcagt gacacgtcgc aacctcgtgt cgtcgcctac
     3901 catttgctgc gcgcgctcgg cgtgcccgac tctcgcgcgc gggacgtcgc cgagcgcccg
     3961 ctgtcggaga tcgtcgctcc gccatagctc ccgttcccga actgcgaaac gggtcaccgc
     4021 gcccgttggc gctcatgcaa gtcatgacaa gccgagggcg acgccgagac gtcgcgtcac
     4081 gtttcctcga cggccgcgga caacggctgg gccaccgtct cgagcggctt gcgttcggcg
     4141 tcgataccga gccacacggc gacgacggcg gcgccgacca ttagcagccc gccgacggca
     4201 tagccgacga acacggcttc gcggtcaccg gtgccgatca gtgcgccgta cagcgcgggg
     4261 ccgacgaatc cgccgaggcc ggtcccgatc gcatagaaga tcgcgatcgc gacggcgcgc
     4321 atctcgagcg ggaagacctc gctgacggtg aggtaggccg agctcgcggc cgccgacgcg
     4381 aagaagaaca gcaccgacca gcagccagtc atcgtcgtcg catcgagcca gtcgttcgcg
     4441 aacgcgtagc ccgacgcgag caacccgacg ccggcgatcc cgtacgtggc ggcgatcatg
     4501 cgccgtcgac cgaccgagtc gaatagccgc cccagcagca gcggtcccaa aaagttgccg
     4561 accgcgaacg ggaagatgta gtagccgacg tcgttggccg cgacgccata aaagctcgtg
     4621 agcacgagcg cgtacgtgaa gaagatcgcg ttgtagaaga acgcctgcga gcccatcaac
     4681 acgaaactga gcacagcccg gcggcggtag cgagtcacga gcagcgtgaa gatctcgcgc
     4741 cacgagacgt gcgaccgcag ccggatcgcg aacgcggtgc gacattcgac gcacgggcct
     4801 tgctcgcgct cgatctccgc ttcgatgcgc gcgacgacgc cttccgcctc ggcgaggcgg
     4861 ccgcgcgtca tgagccatct tgggctctct ggcagatgcc ggcgcacgag caggattgcg
     4921 acggtcagga tcgcgccgaa cgcgaacgcg acccgccaac cggcgtcctc cggcaggcgg
     4981 ccgggctcga gcagcacgat gctcaagccg gcgccggcgg cagcgccgag ccagaagctg
     5041 ccgttgatcg cgagatcgac gaggccgcgc acgcgtgccg gaatcagctc gtcgatcgcg
     5101 gagttgatcg cggcgtattc gccgccgatg ccgaagcccg tcaggaatcg gcagaatgcg
     5161 aacgaccaaa aattccaggt gccggccgtc agcagcgtcg cgacgagata gacggacaac
     5221 gtgacgagga acagcggctt gcggccgagc ttgtcggcca gccgaccgaa gaagagggca
     5281 ccgagcacgg cgcccgcgac gtacgcggag ccggcccagc cgacgtcggc cgcagacagt
     5341 ccgagcgtgc cgtcgcgctg caacgtcggc ccgagcgaac cgaccatcgt gacctcgagc
     5401 ccgtcgagaa cccacgtgat cccgagcgca gtcacgatcc gccagtgcca acgcgaccac
     5461 gggaggcggt cgagccgctc gggaatcgtg gtcgtcaaag cttggccttc caccgtcgtc
     5521 cgatcgcagc tagcgccttg tcttagtgag cccagcggct gcagtttcca tgccgatcag
     5581 cgaggacgag gccgacgatc tcgctcaaga tcgccgcggc ggcgcaactg atcgtcggtg
     5641 tttcgcgcga cctccagaga ttccgcgcgt ccggcgccgc acacacgcgt ggcaagtgct
     5701 cattggtact ttcttcgttc ccagacttgt tgctgataga cgccgccatc aggtcggcga
     5761 tatttcgccg cgttctcttc gttcttggcg accatctgct cgacgtccag cgacgtgtcg
     5821 atctccgact gccagtgctc cctcttgaac gggatgatct gcgcgtacgg tgtgccggcg
     5881 ggcagcactc cgcgggcgcc tttcagccaa aagaacggca ttgtgcccgg caagtgcact
     5941 ttgtcgttgt cgacgattcc gctcgtcgtc aagaacggta gctcgaatcg attgagcggg
     6001 tgcgtgtaga gagcgctgta cccgtcgggt agctcgacgg cccagtcggc ccaccaggca
     6061 aaatgctttt ggtgatagcc ccaagggtgt tggaactgcg gcatcgggtc gcgatcgacg
     6121 agcacgttgc gatactcggg gtccaacact ttggcgtgga tgttgcccgc cgcatcctcg
     6181 gcgaactcga tcgcgcacgg tgtcttgtac gtgtagcccg tgcccatgac gtcgagaacg
     6241 gccgggcacg ccttccacgt cggcattttg ccgctgccgt cttgcgcctc ggccggtttc
     6301 cccgtcgccg ggtccggtac gaaccaatcg gctttacggt accaatccgg tagcgtcttg
     6361 acgatcggct ccggtctcga ggcgctgtct ttagtaagcc aaggtctgtt cgcaacaaat
     6421 ctgatttttt tggtgtccac gtgggtcgcc ttccggggtt ctccagagaa tgactcagcc
     6481 ggcgagcgcg gtcgagcagc gacggcgatc aacgtgttgc atgcgctcac gaccgcagag
     6541 cgccccgctg cggctgtaat aaagatgcct gctcgatcgt atgtttagcg acggagttga
     6601 tgcggagtag ggcaatgcac gagttttcct ggatgtggat cgtcttcgga atgttctttt
     6661 ggctgttgtt gttgcgcccg cgccggctcc gagcctgtca agggc
//
LOCUS       DATLEE010000347         6050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5611042, whole genome shotgun sequence.
ACCESSION   DATLEE010000347 DATLEE010000000
VERSION     DATLEE010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5611042"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VM692_07435"
     CDS             complement(<1..534)
                     /locus_tag="VM692_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HUQ52038.1"
                     /translation="MTKASNQARAFWVAEPGRGEIRTEALRAPAAGEALVRTLFTGVS
                     RGTEALVFNGKVPQSEHERMRAPFQAGNFPAPVKYGYCNVGVVEQGPATLVGRRVFCL
                     YPHQDRYVVPVAALHALPDGLPPERAVLAANLETAVNALWDAAPRIGDRIAVVGAGTV
                     GCLVGWLAARIVGAEVEL"
     gene            617..1372
                     /locus_tag="VM692_07440"
     CDS             617..1372
                     /locus_tag="VM692_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009506318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUQ52039.1"
                     /translation="MATPPLADLDRSAHAPAQRRPRGAVAAVAGAAVATILGASLLAR
                     GLGDDAVVWRGVALALGGGTLLSWLAARHLALPRFGMANAVTLTRAVLVLLLLALLGA
                     QATSALAWLLVALSLAAAVLDGVDGALARSRAETSAFGARFDMEIDALLILVLAALVW
                     QHGKAGPWILLAGLLRYLFVAASYALPWLAAALPPSRRRQTVCVVQIATLIAALVPLI
                     VPPWSAVVAVAGLAALVGSFGIDVVWLQRHARA"
     gene            1383..2999
                     /locus_tag="VM692_07445"
     CDS             1383..2999
                     /locus_tag="VM692_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52040.1"
                     /translation="MNADRPLGARPWLKLAVAVLVLNVALTFHNVWPTLWVKPALELS
                     VELGVLLLALVAGAALVGELGRKTLIVATLIVLALLLGRYAEVTAPALYGRPVNLYWD
                     SQHLPKVAAMLADVGPWWLVGALVLGFIALIAALVGGIAWCLRTVATSLRTAIGRRVV
                     GSVAGALVGVYAVSQLAEWPARYYYALPVAATYLQQLQFVVAAATADVDRDLPSVPLP
                     QSDLGRLQGDDVLLMFLESYGAVTYDEPKIARAVAPARDELAHAAAATGRHIVSAYAS
                     SPTFGGGSWLAHASFMSGVEVRDTGDYMVLLTQERTTWPKLFKGAGYRAIAVMPGLKT
                     AWPEGSFYGFDSIQDELALSYGGPEFGWWRIPDQFSLARVTELEAANDAHGPLLTFFP
                     TINTHIPFLPVPPYQADWQRVARSEPYPAEDVEASLAQVPDWEALGGPYADSFVYTFT
                     YLAGYLHARPHASEILVLLGDHQPAASVAGVGARWDVPVHVVTSRDDIAASLIAAGFV
                     EGVALAPLQRPIATLPELSALLLETFAAKH"
     gene            complement(3001..3750)
                     /locus_tag="VM692_07450"
     CDS             complement(3001..3750)
                     /locus_tag="VM692_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HUQ52041.1"
                     /translation="MSALARAFGIARSLAIYYGIPLRAGRLRRFYAQFVQPGSLCFDV
                     GAHAGNRVRCWRQLGARVVAIEPQPDFVRILRWLYGHDRDVEIVPLAVGRNLGHAELL
                     VSERTPTVTTLSREWVDTVQRDPRFAGVAWARGERVEVVTLESLIERYGEPQFIKVDV
                     EGFEAEVLAGLTTPLRGLSFEYLPATRDVALDCIERLEALARYRYNWSPGESHRLASA
                     AWLDAAGMRALLMGLPQLAASGDVYAVRADV"
     gene            3898..5190
                     /locus_tag="VM692_07455"
     CDS             3898..5190
                     /locus_tag="VM692_07455"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUQ52042.1"
                     /translation="MRAQPAALEVPFRQFELANGLNVILHRDTSVPVVAINMWYHVGS
                     GSERPGRTGFAHLFEHLMFEGSKNVKEGEFDTLLEAAGANNNGSTSNDRTNYIIDVPA
                     NGLELALFLESDRMAYLLDTMTPARVDGQRDVVKNERRQSYENRPYGMASIVLDELLW
                     PDGHPYHWPTIGYMEDLTAASHDDVVQFFKTYYAPNNASLVVAGDIDLDAAQALVTKW
                     FSEIPRGPEVLPIAPPAARLTSVERRTLTDRVRLPRLHLAWLTPRRFAPGDAALDVLG
                     AVLAGGKNSRLYKRLVYDTQIAQDVIAYQEPGALGNSFRIIATARPGHTAAELGAAID
                     EEIARVQQEPPSAREVERAQNQIEASFYRAMERVGGFSGKADQLNDYFASGGGPDFFA
                     EDLARYASLTAGDVQSAAREWLPKHRRVEVVVEPEAVP"
     gene            5187..>6050
                     /locus_tag="VM692_07460"
     CDS             5187..>6050
                     /locus_tag="VM692_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUQ52043.1"
                     /translation="MKALLTLAFALAITAASAQQGPNRSSPPALGPQPRLTLPAVQER
                     KLANGLRVLLLEAHEVPLVQINLLFDAGSADDSTGQFGIASFTAAMLDEGAGARSALE
                     IADAVEYLGAELGTSSSFDASAVRLNVPTRRLEQALPIMADVALRPTFPAAELERLRQ
                     ERLTALIQTRDDPAGVAPPAFARVVYGATHRFGTSTIGTTATLEAVTDEQLRAFHAAR
                     YRPDNATLIVVGDVTPASVLPLLESAFGGWRADRSPARAQLPAASPPAEREITIVDIP
                     GAEQSQIRIGTV"
BASE COUNT          889 a         2098 c         2092 g          971 t
ORIGIN      
        1 gagctcgacc tcggcgccga cgatgcgcgc ggcgagccag ccgacgagac agccgacggt
       61 cccggcgccg acgacggcga tgcggtcgcc gatgcgcggc gcggcgtccc acagcgcgtt
      121 gacggccgtt tcgagattgg ctgccagcac ggcgcgctcg ggcggcagcc cgtcgggcaa
      181 cgcatgcagc gcggcgacgg gcacgacgta acgatcctga tgcggataaa gacagaacac
      241 gcgtcggcca acgagcgtcg ccggaccctg ctcgacgacg ccgacgttgc agtagccgta
      301 tttgacgggc gccggaaagt tgccggcctg gaacggcgcg cgcattcgct cgtgctcgct
      361 ctgcggcact ttgccgttga acacgagcgc ctcggtgccg cggctcacgc ccgtgaataa
      421 cgtccgcacg agcgcttcac cggcggccgg cgcgcgcaag gcttccgtcc gaatctcgcc
      481 acggccgggc tcggccaccc agaacgcgcg cgcttgattg gaagctttcg tcattggctg
      541 accgtgaccg ccggcccgac gcggcctgcg cgggcatact tgcaaaccgg tcggcaggca
      601 tgcaagcgtc aaccgtatgg cgactccacc gctagccgat ctcgaccgca gcgcgcacgc
      661 gccggcgcag cgccggccgc gcggcgcagt cgctgccgtc gcaggcgcag cggtggctac
      721 gatcctcggc gcgtcgctgc tcgcgcgcgg gctcggcgac gacgccgtgg tctggcgcgg
      781 cgtggcgctg gccctcggcg gcggcacctt attgtcgtgg ctcgcggcgc gccatctagc
      841 gctgccgagg ttcggcatgg cgaacgccgt gacgctgacg cgcgcagtgc tcgtgctgct
      901 gttgctcgca ctgctcggcg cgcaggcaac gagcgctctc gcttggctgc tcgtcgcgtt
      961 gagcctcgcg gccgcggtgc tcgacggcgt cgacggcgct ttggcgcgca gccgcgctga
     1021 aaccagcgcg ttcggcgccc gcttcgacat ggagatcgac gcgctgctga tcctcgtgct
     1081 cgcggcgctc gtgtggcagc acggcaaggc cggcccgtgg atcttgctcg cgggcttgtt
     1141 gcgctatctg ttcgtcgccg cgagctacgc gctgccgtgg ctcgccgcgg cgttgccgcc
     1201 gagccgccgc cgccaaacgg tgtgcgtcgt gcagatcgcg acgttgatcg ccgcgctcgt
     1261 gccgctcatc gtgccgccgt ggagcgctgt cgtggctgtc gcgggtctcg cggcgctcgt
     1321 cgggtcgttc ggcatcgacg tcgtttggct acagcgccac gctcgcgcct aacatggccg
     1381 ccatgaacgc agatcgaccg ctcggagcgc ggccctggct caagttggcc gtcgcggtgc
     1441 tcgtgttgaa cgtggcgctg acgtttcaca acgtttggcc gacgctgtgg gtcaaacctg
     1501 cgctcgagct ttcagtcgag ctcggcgtac tgttactggc gctcgttgcg ggtgccgctc
     1561 tcgtcggcga gctcggccgc aagacgctga tcgttgcgac actgatcgtt ctagcgctac
     1621 tgctcggacg ctacgccgag gtcaccgcgc cggccctgta tgggcggccc gtgaacttgt
     1681 attgggacag tcaacacctg ccgaaggtcg cggcgatgct tgccgacgtc gggccgtggt
     1741 ggctcgtcgg cgcgctcgtg ctcggcttta tcgcgctgat tgcggctctc gtcggcggca
     1801 tcgcctggtg tctacggacc gtagcgacct cgctgcgcac ggcgatcggc cgccgcgtcg
     1861 tcggctccgt cgccggcgcg ctcgtcggcg tctatgcggt gagccagctc gcggagtggc
     1921 cggcgcgcta ctactacgct ctgcccgtcg ctgcgacgta tctacagcag cttcagttcg
     1981 tcgtcgcggc tgcgacggcc gatgtggacc gcgacctgcc ttccgtgccg ttgccgcagt
     2041 ccgacctcgg gcggcttcaa ggcgacgacg tgttgctgat gttcctcgag tcgtacggtg
     2101 ccgtgaccta cgacgagccg aagattgcgc gcgctgtcgc gccggcgcgc gatgagctcg
     2161 cacacgcggc cgcagcgacg ggccggcaca tcgtgtcggc ctacgcttcg tcgccgacgt
     2221 tcggcggcgg ctcctggctc gcgcacgcga gcttcatgag cggtgtcgag gtccgcgaca
     2281 cgggcgacta catggtgctg ctcacgcagg agcgcacgac gtggccgaag ctgttcaaag
     2341 gcgccggcta tcgcgcgatc gccgtcatgc cgggcctgaa gacggcgtgg ccggagggct
     2401 cgttttacgg cttcgattcg attcaggacg agctcgcgct cagctacggc ggccccgagt
     2461 tcggctggtg gcggatcccg gatcagttct cgctggcgcg tgttaccgag ctcgaggcgg
     2521 cgaacgacgc gcacgggccg ctcctgacct tcttcccgac gatcaacacg cacattccgt
     2581 ttctacccgt gccgccgtat caggcggact ggcaacgcgt cgcgcgatcc gagccgtacc
     2641 cggccgagga tgtcgaagcc agtcttgcgc aggtcccaga ctgggaagca ctcggcgggc
     2701 cgtatgccga ctcgttcgtc tatacgttca cgtacctggc cgggtatctg cacgcgcgcc
     2761 cgcacgcgtc cgagatcctc gtgctgctcg gggatcacca gccggccgcc agcgtggccg
     2821 gcgtcggcgc gcgctgggac gtgccggtgc acgtcgtgac gagccgcgac gacatcgccg
     2881 catcgctgat cgccgcaggc ttcgtcgagg gcgtcgcgtt ggcgccgcta cagcgcccga
     2941 tcgcgacgtt gcctgagcta tcggcgctgc tgctcgagac gttcgccgcg aagcactagc
     3001 tcaaacgtcg gcgcgcaccg cgtagacgtc gccggacgcc gccagttgcg gcaggcccat
     3061 cagcaacgcg cgcatgcccg cggcgtcgag ccaagcagcg ctcgcgagcc gatgcgattc
     3121 gccgggcgac cagttatagc ggtaccgcgc cagcgcctcg agccgctcga tgcaatcgag
     3181 cgcgacgtcg cgcgtggccg gcagatactc gaacgacaat ccgcgtagcg gcgtcgtcag
     3241 gcccgcgagc acttccgcct cgaagccttc gacatcgact ttgatgaact gcggctcgcc
     3301 atagcgctcg atcaaagact cgagcgtgac gacctcgacg cgctcgccgc gcgcccaggc
     3361 gacgccggcg aagcgcggat cgcgctgcac ggtatcgacc cattcgcgcg acagcgtcgt
     3421 gacggtcggc gtgcgctcgc tgacgagcag ctcggcgtga cctaagttgc ggccgaccgc
     3481 gagcggcacg atctcgacgt cgcggtcgtg gccgtagagc cagcgcagga tgcgcacgaa
     3541 gtcgggctgc ggctcgatcg cgacgacgcg cgcgccgagc tgccgccaac agcgcacgcg
     3601 gttgccggca tgcgcgccga cgtcgaagca gagactgccg ggctgcacga actgcgcgta
     3661 gaagcgccgc aagcggccgg cgcgcaacgg gatgccgtag tagatcgcca gcgagcgtgc
     3721 aatgccgaaa gctcgtgcca gggcgctcat tggcggcgat tctaacggac ggcgccgctt
     3781 tcgcgtcttg tcctcgagct tgcgcctcgc gcaagatcga agcggctacc gcgggagcgt
     3841 gcgatgcgtt gtggatttgc tctgcttggc ttagtcgtct cggctgctgc tgtgcccgtg
     3901 cgcgcacaac cggccgcgct cgaggttccg ttccgccaat tcgagctcgc caacgggctc
     3961 aacgtcattt tgcatcgcga cacgagcgtg cccgtcgtcg ccatcaacat gtggtatcac
     4021 gtcggttcgg gcagcgagcg gccgggccgc acgggcttcg cgcatttgtt cgagcatttg
     4081 atgttcgaag gctcgaagaa cgtcaaggag ggcgagttcg acacgttgct cgaggccgcg
     4141 ggcgccaaca acaacggctc gaccagcaac gaccgcacga actacatcat cgacgtgccg
     4201 gcgaacgggc tcgagctcgc gctgttcctc gagtcggatc gcatggcgta tctgctcgac
     4261 acgatgacgc cggcgagagt cgacggccag cgcgacgtcg tcaagaacga gcggcgacag
     4321 agctacgaga acaggccgta cggcatggcc tcgatcgtgc tcgacgagct gctgtggccc
     4381 gacggccacc cgtaccactg gccgacgatc ggctacatgg aagatctcac ggcggccagc
     4441 cacgacgacg tcgtgcagtt cttcaagacg tactacgcgc caaacaatgc gagcctcgtc
     4501 gtcgccggcg acatcgacct cgacgccgcg caagcgctcg tcacgaaatg gttcagcgag
     4561 attccgcgcg ggcccgaagt gctgccgatc gcgccccccg ccgcgcggct tacgagcgtc
     4621 gagcggcgca cgctgaccga ccgcgtgcgc ctgccgcggc tgcatctcgc gtggttgacg
     4681 ccgaggcggt tcgcgcccgg cgacgcggcg ctcgacgtcc tcggcgccgt gcttgcgggc
     4741 ggcaagaact cgcggctcta caagcggctc gtctacgaca cgcagatcgc gcaagacgtc
     4801 atcgcgtacc aagagcccgg cgcgctcggc aactcgttcc gcatcatcgc gacggcgcgg
     4861 ccgggccata cggcagcgga gctcggcgcg gcgatcgacg aagagatcgc gcgcgtgcaa
     4921 caagagccgc cgtcggcgcg tgaggtcgag cgcgcgcaaa accagatcga agcgtcgttt
     4981 taccgcgcca tggagcgcgt cggcggcttt tccggcaagg ccgatcagct caacgactat
     5041 ttcgcgagcg gcggcggccc ggatttcttc gccgaggatc ttgcgcgcta cgcgtcgctg
     5101 acggccggcg atgtgcagtc ggcggcccgc gagtggctgc ccaagcaccg gcgcgtcgaa
     5161 gtcgtcgtcg agccggaggc cgtgccatga aagcgttgtt gactctggcc ttcgcgctag
     5221 cgatcacggc ggcgtcggcg cagcagggtc caaatcggtc ctcgccgccg gcgctgggac
     5281 cgcagccgcg actgacgttg ccggccgtgc aagagcgcaa gctcgcgaac ggcctgcgag
     5341 tgctgctgct cgaggcgcac gaagtgccgc tcgtgcaaat caacttgctg ttcgatgccg
     5401 gcagcgcaga cgactcgacg ggccagttcg gcatcgcgag cttcacggcc gcgatgctcg
     5461 acgaaggcgc cggcgcgcgc agcgcactgg agattgccga cgccgtcgag tatctcggtg
     5521 ccgaactcgg cacgtcgagc tcgttcgacg cctcggccgt ccgcttgaac gtgccgaccc
     5581 gccggctcga gcaagcgctg ccgatcatgg ccgacgtcgc gttgcggccg acgtttccgg
     5641 cggccgagct cgagcgtctg cggcaagagc ggctgaccgc gttgatccaa acacgcgacg
     5701 acccggccgg cgtcgcgccg ccggcgttcg cgcgcgtcgt gtacggcgcg acgcatcggt
     5761 tcggcacgag cacgatcggc actacggcga cgctcgaggc cgtgaccgac gagcagctac
     5821 gcgcgttcca cgcggcgcgc tatcggcccg acaacgcgac gttgatcgtc gtcggcgacg
     5881 tcacgcccgc gtcggtgctg ccgctgctcg agagcgcgtt cggcggctgg cgggccgacc
     5941 ggtcgccggc gcgcgcgcaa ctgccggcgg cgtcgccacc cgccgagcgc gagatcacga
     6001 tcgtcgacat cccgggcgcc gagcaatcgc agattcgcat cggcaccgtc
//
LOCUS       DATLEE010000348         7101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4101802, whole genome shotgun sequence.
ACCESSION   DATLEE010000348 DATLEE010000000
VERSION     DATLEE010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4101802"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1015)
                     /locus_tag="VM692_07465"
     CDS             complement(173..1015)
                     /locus_tag="VM692_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HUQ52044.1"
                     /translation="MSSRPSVKRKERPPRRGLAALLVGLGVVGVALADPMPFRQYPGV
                     EYYDFELPTDFRQPAEWVFGRLMYPSVGFGGQWKLGSTSWTIDYPRGDRHIAALVRRL
                     SIIDARSVEQPVNLEDDDDVFNWPWLYAVEVGQWDLSEEQAAKLREYLLRGGFLMVDD
                     FHGTEQWAIFVESMRRVFPDRPIVDLPDDDPIFHTFWDLVDRKQIPGLQFLYSGRTYE
                     DNGFDAHWRAIYDDQGRVMVAICHNMDLGDAVEHSDTRTYPEEYSATAMRTFLNYIVY
                     AMTH"
     gene            1105..2484
                     /locus_tag="VM692_07470"
     CDS             1105..2484
                     /locus_tag="VM692_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HUQ52045.1"
                     /translation="MKDLTQGPIVTNILAMAAPMAAGMIFQTLYFLVDLYFVAGLGDA
                     SVAGVGAAGTLMFVIMALTQVLGVGAVALIAQAVGRRDQADANLVFNQSVLFAVACAV
                     ATLIGGYAFAGAYVATIAADVTTQAEGVTYLYWFLPGLALGFAQVVMGSALRGTGIVK
                     PTMAIQALTVLLNTLLAPVLIAGWGTGYAMGVAGAGLASSVSIVIGIVLLGAYFLKLE
                     KYVSFDPHLWRPQLRVWKRMLDIGLPAGGEFALMFIYMGVIYWVISDFGAAAQAGFGI
                     GSRIMQSIFMPAMAIAFAAGPIAGQNFGAKQGARVRETFVKALLLNTIVMVLVTAFLQ
                     WRPEVLVAFFTPEAEAQAVGATFLRIISWTFIAQGVVFTCSGVFQGLGNTIPAMLSSA
                     TRLAVFVPGAVWLSAQPAFHLDEVWYLSVSTVWLQGIVSYLLMRHQFKRRLAEAPPPP
                     AAVVAPAET"
     gene            2557..2931
                     /locus_tag="VM692_07475"
     CDS             2557..2931
                     /locus_tag="VM692_07475"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52046.1"
                     /translation="MRTRLLVGLTGTALGLVALQAAAHHGWAGQQTEQFELTGKVQTD
                     VSLAGPHGTMQIVDDKGQVWDITLAPAARTERAGLKPGVLPMGATVTVSGHRSSDPKR
                     FEVKTERVTHNGKNYDVYPDRL"
     gene            2951..3436
                     /locus_tag="VM692_07480"
     CDS             2951..3436
                     /locus_tag="VM692_07480"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HUQ52047.1"
                     /translation="MEALLLWLEGSALGQLMRASGVWAYGLVNLAHILGIATLFGSVL
                     VLDLRLLGAWRRVPLAALEPPALTLAACGFILAVASGISLLTTNASEYVGNPFLPIKF
                     TAIGLGLLNVIAVQFVPAWQARRSEPHDARQRLALGFVGGTSLVCWTAAVAAGRMLGY
                     W"
     gene            3507..4031
                     /locus_tag="VM692_07485"
     CDS             3507..4031
                     /locus_tag="VM692_07485"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HUQ52048.1"
                     /translation="MSTSNAWAPSRGAADDVGRVDEADELELAGPSARLGAFFVDGIV
                     GFVMVWLPALIVLLATGGVDAFDPEVFTLDIDVYGVAALLCFTGLVSWCWVTALLVAR
                     SGQTIGKRLLDIKVVRSDGSHATLERIFWRRNVVNWLLGVIPLYGLVDLLFIFGARQQ
                     CIHDLVADTIVVKA"
     gene            4042..4746
                     /locus_tag="VM692_07490"
     CDS             4042..4746
                     /locus_tag="VM692_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HUQ52049.1"
                     /translation="MAHAVLDTLVAAETPEGILLELRPAGLSARFYAFLLDWLIRLLI
                     AYVAGLAATFMGGFGMGFWLLLYFVLEWFYPVVFELSRSGATPGKSVVGIKVVMDDGL
                     PITPAASFTRNLLRVADFLPFLFGAAIVAMLLRKDCKRLGDLAAATLVVHQPQRPPKV
                     KLDGLTPVIPVVPLSPRDQAAIVALAARAPTLTGERLDELAALAAGVSGDGGKAGPNV
                     TRRVLGVALWAMGRRA"
     gene            4743..5723
                     /locus_tag="VM692_07495"
     CDS             4743..5723
                     /locus_tag="VM692_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HUQ52050.1"
                     /translation="MTPLKFEELYQAEWQELEDLLDRVLGRKKKAASAEPLRGERIAT
                     LYRRACEQLALARARSYPAYLVDRLDRLTADAHQVIYQQREFGAGALWRIFARDFPRA
                     VRADAVFVSIAAALFVIPTIVLGVVVYYRPDLILSVVDAATAAQFEQMYSTVAESIGR
                     TRDAGDDWTMFGFYIRNNVGVAFQCFASGLAAGIGSIFYLVYNGAMGGAVAGYLTERG
                     LGETFYSFVVTHGSFELTAIVLSGAAGLKLGHSLLAPGRRTRGQSLVVAAKDCVVIVY
                     GVAAMLVVAAALEAFWSSSAWVPHGVKYGVAAVCWLAVFAYLALQGRDAA"
     gene            5713..6447
                     /locus_tag="VM692_07500"
     CDS             5713..6447
                     /locus_tag="VM692_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52051.1"
                     /translation="MQLDSLALRMRPRAPLEAADLGARLCQDAARSVYACYALAYVPV
                     AALALASFELAAWLPTLVLFFAKPWLDRTILFVLSRAAFGQATTVLQLWEAQRQVWWS
                     QLFLTLTWRRLSPWRSFTQPVYQLEGLALNKIRRRVVQIRRRKRGTALMITTAFATAE
                     TCLVTTVLSLIIWFAPGDTAPDLGTLLFDETNVLLEAASAVAYAIVIAFLEPFYVAAG
                     FAMYLNRRAELEAWDVEQELRRAFAA"
     gene            6431..>7101
                     /locus_tag="VM692_07505"
     CDS             6431..>7101
                     /locus_tag="VM692_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HUQ52052.1"
                     /translation="MRLLRNLRLELRLAVVVALAPLGAPAQPEPAADVEPVPRAEVRK
                     ALDKVANDPNLVAERSVNMLRWAEAKPVTDEPWWWGLINGLARWFRGLFAWIAESGRY
                     VVWVIGALLAAVLVTYVVRLVRVRGMARVPKTFAPPSHVRDLDIRPESLPDDVGSAAL
                     ALWNRGEQRAALALLYRGLLSRLVHVHSVPIRASSTEGECVALARPRLAEANARYATR
                     LVDTWG"
BASE COUNT         1010 a         2326 c         2390 g         1375 t
ORIGIN      
        1 tgcgccgagc tcccgcgcga agaaggccag gacgcccgtg ccggcgccga tgtcggcgac
       61 cgtgctcgag cctggacgga tcgcgtgctc gagcgccgca cgaaatgctt cgtggcggac
      121 gcggtcggcc agcatgcgac gatggaattc gatcgacatg cctggcggtc ggtcaatgcg
      181 tcatcgcgta gacgatgtaa ttcaagaacg tgcgcatcgc cgtggccgag tactcttcgg
      241 gataggtccg agtatccgag tgctcgacgg catcgcccaa gtccatgttg tggcaaatcg
      301 cgaccatcac gcgcccttgg tcgtcgtaga tggcgcgcca atgcgcatcg aagccgttgt
      361 cctcgtacgt ccggccgctg tagaggaact gcagaccggg aatctgtttc cggtcgacga
      421 gatcccagaa tgtgtgaaag atcggatcgt cgtcgggcag atcgacgatc ggccgatcgg
      481 gaaacacgcg ccgcatgctc tcgacgaaga tcgcccactg ctcggtaccg tggaaatcgt
      541 cgaccatcaa aaagccgccg cgcagcaggt attcgcgtag cttcgcggct tgctcttcgg
      601 agaggtccca ctggccgacc tcgacggcgt acagccacgg ccaattgaag acgtcgtcgt
      661 cgtcctcgag attgacgggc tgctcgaccg agcgcgcgtc gatgatgctg aggcgccgca
      721 cgagcgcggc gatatgtcgg tcgccccttg ggtagtcgat cgtccagctc gtgctgccga
      781 gcttccattg gccgccgaac ccgaccgatg ggtacatgag gcgcccgaag acccactcgg
      841 ccggctgacg gaagtcggtc ggcagctcga aatcgtagta ttcgacgccc gggtattgcc
      901 gaaacggcat ggggtctgcc aacgcgaccc cgacgacgcc gaggcccacc aacaacgcag
      961 ccaggccgcg gcggggaggg cgttccttgc gtttgacgga cggccttgag ctcaccgtcc
     1021 gagcataaca actcgtctcg cgcccggact agagcactga cacgctagcg agtattcttt
     1081 gcgacccgtt gcgcgaggcc tcccatgaag gatctgaccc aaggcccgat cgtcacgaac
     1141 atcctcgcaa tggccgcgcc gatggcggcc ggcatgattt tccagacgct gtacttcctc
     1201 gtcgacctct acttcgtcgc gggccttggg gacgcttccg ttgcgggcgt cggcgcggcc
     1261 ggcacgctga tgttcgtgat catggcgttg acgcaggtgc tcggcgtcgg cgccgtcgcg
     1321 ctgatcgcac aggccgtcgg ccgccgcgat caggccgatg ccaacctcgt gttcaaccag
     1381 tcggtgctgt tcgctgtcgc gtgcgcggtc gcgaccttga tcggcggcta tgccttcgca
     1441 ggcgcgtacg ttgcgacgat tgcggccgat gtgacgacgc aggccgaggg cgtgacgtat
     1501 ctctattggt tcctgcctgg cttggcgctc ggtttcgcgc aagtcgtcat gggctcggcg
     1561 ctgcgcggca ccggcatcgt gaagccgacg atggcgatcc aagcgctgac cgtgctgctg
     1621 aacacgttgc tcgcgccggt gctgatcgcc ggctggggaa ccggttatgc catgggcgtc
     1681 gcgggcgcgg gcctcgcgag ctcggtctcg atcgtcatcg gcatcgtctt gctcggcgcg
     1741 tacttcttga agctcgagaa gtacgtcagc ttcgatccgc acctctggcg gcctcagctg
     1801 cgggtctgga agcgcatgct cgacatcggc ttgccggccg gcggcgagtt cgcgctgatg
     1861 ttcatttata tgggcgtcat ctactgggtc atcagcgact tcggcgcggc cgcccaggcc
     1921 gggttcggca tcggctcgcg catcatgcag tcgatcttca tgccggcgat ggcgatcgcg
     1981 ttcgcggccg gcccgatcgc gggccagaac ttcggcgcca agcagggcgc ccgcgttcgc
     2041 gagacgttcg tcaaggcgct gctgctgaac accatcgtca tggtgctcgt gaccgcgttc
     2101 ttgcaatggc ggcccgaggt gctcgtcgcg ttcttcactc ccgaggccga ggcacaagcc
     2161 gtcggcgcga cgttcttgcg catcatctcg tggaccttca tcgcgcaggg cgtcgtgttc
     2221 acttgctcgg gtgtcttcca aggtctcggc aacacgattc ccgccatgtt gagctcggcg
     2281 acgaggctcg ccgtgttcgt gccgggggca gtgtggttgt cggcgcagcc tgcgttccac
     2341 ctcgacgagg tctggtatct ctcggtctcg accgtctggc tgcagggcat cgtcagttat
     2401 ctgttgatgc gccatcaatt caagcggcgg ctcgccgagg cgccgccccc gccggcggcc
     2461 gtcgtcgcgc ccgcagaaac ctgaagccgg ttcgcctcgg ctcacgatcg cggtaccatc
     2521 gaagctcgtc ctacggagag ctttcaggag gcagccatgc gcacgcgatt actcgttggt
     2581 ttgacgggca ccgcactcgg gctcgtcgcg cttcaagcag ccgctcatca cggttgggca
     2641 gggcaacaga ccgaacagtt cgagctcacg ggcaaggtgc aaacggatgt cagtcttgcc
     2701 ggtcctcacg ggacgatgca gatcgtcgac gacaaagggc aggtgtggga catcacgctc
     2761 gcgccggccg ctcgcaccga gcgagctggg ctcaagcccg gcgttttgcc gatgggcgcc
     2821 acggtcaccg tcagcgggca tcgcagcagc gacccgaagc gcttcgaggt caagaccgag
     2881 cgcgtcacgc acaacggcaa gaactacgac gtctatcccg accggctctg acgcctaagt
     2941 cggctcgcgt ttggaagcgc tgctcctatg gctcgagggc tctgccctcg ggcaactcat
     3001 gcgcgcatcc ggcgtgtggg cgtacggcct cgtcaatctc gcgcacattc taggcatagc
     3061 gaccctgttc ggctcggtgc tcgtgctcga cctgcgtctg ctcggcgcgt ggcgccgcgt
     3121 gccgctggca gcgctcgagc cgcccgcgct gacgctcgca gcgtgcggtt tcatcctcgc
     3181 cgtcgcgagt ggcatctcgt tgctgacgac gaacgccagc gagtatgtcg gcaatccgtt
     3241 tctgccgatc aagttcacgg cgatcggtct cggcttgctg aacgtcatcg ccgtgcagtt
     3301 cgtccccgct tggcaagcgc gtaggtcgga gccgcatgac gcgcgtcagc gtctcgcgct
     3361 cgggttcgtc ggcggcacgt cgctcgtttg ttggacggcc gccgtcgcgg cgggccgcat
     3421 gctcgggtat tggtgaggtt gcacgacacc gtgccgcgaa atcgggcaga atcgcggcgt
     3481 tctcgggcct cacaggggag ctttgcttga gcacgagcaa cgcgtgggcg ccttcgcgtg
     3541 gtgccgccga cgacgtcggc cgggtcgacg aggccgacga gctcgagctc gccggcccaa
     3601 gcgcgcggct tggcgccttt ttcgtcgacg gcatcgtcgg cttcgtgatg gtgtggctgc
     3661 ccgcgctgat cgtgctcctc gcgaccggcg gcgtcgacgc gttcgatccc gaggtattta
     3721 cgctcgacat cgatgtttac ggcgttgcgg ccctgctgtg cttcacgggc ctcgtcagct
     3781 ggtgctgggt cacggcgctg ctcgtcgcgc gcagcggcca gaccatcggc aagcgattgc
     3841 tcgacatcaa ggtcgtgcgc agcgacggct cccacgcgac ccttgagcgg atcttttggc
     3901 ggcgcaatgt cgtgaactgg ctgctcggcg tgattccgct ctacggcttg gtcgatttgc
     3961 tattcatttt cggcgctcgg caacaatgca tacacgacct cgtagccgac acgatcgtcg
     4021 tcaaagcctg agccgctgcc gatggcgcac gctgtgctcg acacgctcgt ggccgccgag
     4081 acgcccgagg gcatactgct cgagctgcgc cccgcaggcc tcagcgctcg gttctatgcg
     4141 tttctgctcg attggctgat caggctgctc attgcctacg tcgccggcct cgcggccacc
     4201 ttcatgggcg ggttcggcat ggggttctgg ctgctgttgt acttcgtgct cgagtggttc
     4261 tacccggttg tgttcgagct cagccgctcc ggtgcgacgc cgggcaagag cgtcgtcggc
     4321 atcaaggtcg tcatggacga tggtctgccg atcacgccgg ccgcatcgtt cacgcgcaat
     4381 ttgctgcgcg tcgctgactt cttgccgttc ttgttcggcg cagcgatcgt cgcgatgctg
     4441 ctacgcaagg actgcaagcg tctcggcgat ctcgcggcgg cgacgctcgt cgttcaccaa
     4501 ccgcaacggc cgcccaaggt gaagctcgac ggcctcacgc cggtgatccc cgtcgtgccg
     4561 ctctcgccgc gcgatcaggc tgcgatcgtc gcgctcgcgg cgcgcgctcc gacgctgacc
     4621 ggcgagcggc tcgacgagct ggcggcgctg gcggcaggcg tctcgggcga cggcggcaaa
     4681 gcagggccga acgtcacgcg ccgcgtgctc ggcgtggcgt tgtgggcgat gggccgccgc
     4741 gcatgacgcc gctcaagttc gaggagctgt atcaggccga atggcaagag ctcgaagacc
     4801 tgctcgaccg agtgctcggg cgcaagaaga aagctgcctc ggcggagccg ctccgcggtg
     4861 agcgcatcgc gacgttgtac cggcgagctt gcgagcagct cgcgctggcg cgggcccgct
     4921 cgtatccggc ctatctcgtc gatcgtctcg atcgactcac ggccgacgcg caccaggtca
     4981 tctatcagca acgcgaattc ggcgccggtg cactgtggcg aatcttcgcg cgcgactttc
     5041 cgcgcgcagt gcgcgcagat gccgtctttg tctcgatcgc cgccgcgctg ttcgtgatac
     5101 cgacgatcgt gctcggagtg gtggtctact accggcccga tctgattctg tccgtcgtcg
     5161 atgcggcgac ggccgcgcag ttcgaacaga tgtactcgac ggtcgccgag tcgatcggcc
     5221 gcacgcgcga cgccggcgac gattggacga tgttcggctt ctacatcagg aacaatgtcg
     5281 gcgtcgcgtt ccagtgcttt gcgagcggcc tcgcggcggg catcggcagc atcttctatc
     5341 tcgtctacaa cggcgcgatg ggcggcgcgg tcgcgggcta tctgaccgag cgcggcctcg
     5401 gcgagacgtt ctactcgttc gtcgtgacgc atggatcgtt cgagctcacg gccattgttc
     5461 tgtcgggcgc cgccggccta aagctcggcc attcgctgct tgcgccgggc cgccgcacgc
     5521 gcggccagag tctcgtcgtc gcggccaagg actgcgtcgt catcgtctac ggcgtcgcgg
     5581 ccatgctggt cgttgccgcg gcgctcgagg cgttctggtc gtcgtcggcg tgggtgccgc
     5641 acggcgtgaa gtacggcgtc gccgccgttt gctggctcgc cgttttcgcc tacctcgcgt
     5701 tgcagggccg cgatgcagct tgactcgctg gcactgcgaa tgcggccgcg cgcgccgctg
     5761 gaggccgccg atctcggcgc gcgcctctgc caagacgccg cgcgctcggt ctatgcgtgc
     5821 tatgccctgg catacgttcc cgtcgcggcg ctggccttgg cttcgttcga gctcgcggct
     5881 tggttgccga cgctcgtgtt gttcttcgcg aagccgtggc tcgatcgcac gatcctattc
     5941 gtgctctcgc gcgctgcctt cggccaagcg acgacggtgc tccagctttg ggaggcccag
     6001 cgccaggtat ggtggagcca gctgttcctg actttgactt ggcggcgctt gtcgccttgg
     6061 cggtcgttca cgcagcccgt ctatcagctc gaaggcttgg ccttgaacaa gatccgccga
     6121 cgcgtcgtgc agatcaggcg ccgcaagcgc ggcacggcgc tgatgatcac gacggcgttc
     6181 gcgacggccg aaacttgcct cgtcacgacg gtgctgtcgc tgatcatctg gttcgcgccc
     6241 ggcgacacgg cgcctgacct aggcacgctg ctgttcgacg agacgaacgt gttgctcgag
     6301 gccgcgtcgg ccgtcgccta cgcgatcgtg atcgcgttcc tcgagccgtt ctatgtagcg
     6361 gccgggttcg cgatgtatct gaaccggcgc gccgagctcg aggcgtggga tgtcgagcag
     6421 gagttgcggc gtgcgtttgc tgcgtaactt gcggctcgag ctccggctcg ctgtcgtcgt
     6481 cgcgctcgcg ccgctcggcg cgccggcgca acccgagccg gcggccgacg tcgagcccgt
     6541 cccgcgcgcc gaggtccgca aagcgctcga caaggtcgcg aacgatccga atctcgttgc
     6601 ggagcgcagc gtcaacatgc tgcgctgggc cgaggcgaag cccgtcacgg acgagccgtg
     6661 gtggtgggga ctgatcaacg gcttggcgcg ctggttccgt ggcttgttcg cgtggatcgc
     6721 ggagtcgggc cgctacgtcg tgtgggtcat cggcgccttg ttggccgcgg tgctggtgac
     6781 ctacgtcgtg cggctcgtgc gcgtgcgagg tatggcgcgc gtgccaaaaa cattcgctcc
     6841 gccgagtcac gtgcgagacc tcgacattcg tcccgagagc ttgcccgacg acgtcggctc
     6901 cgccgcgctc gcgctctgga accgcggcga gcaacgtgcg gcgctcgccc tgctgtatcg
     6961 cggcctgctg tcgcgactcg tgcacgtgca cagcgtgccg attcgcgcgt catcgaccga
     7021 aggcgagtgt gttgcgctcg cgcggccgcg gcttgcggag gcgaacgcac gctatgccac
     7081 gcgtctcgtc gacacgtggg g
//
LOCUS       DATLEE010000349         3372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9335939, whole genome shotgun sequence.
ACCESSION   DATLEE010000349 DATLEE010000000
VERSION     DATLEE010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9335939"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VM692_07510"
     CDS             <1..618
                     /locus_tag="VM692_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ52053.1"
                     /translation="RTTLQLPGAGGGVNWGGVAADARNGIVYMHTHDTSLVGWIEERK
                     PGKNYGRGVAGTTIPYDRGSVNGPGPYFTFSAQAKNDAGESLGSLPCYRPPWSRLVAF
                     DANRGEILWQSVLGVTPGLPAARQLTGGSGNAGPTATAGGLVFVGSTNDKRFRAFDAK
                     NGKEVWSVQLDAVVNANPMTYRGRDGKQYVAFVADDTLRAFALPK"
     gene            713..931
                     /locus_tag="VM692_07515"
     CDS             713..931
                     /locus_tag="VM692_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52054.1"
                     /translation="MDGLTWFGFLSLTGMLICYALEDRSHWYMLGFTIGCVLGSVYGF
                     LQGAWPFGVVEAIWSVVAFQRWRRVRVA"
     gene            964..1329
                     /locus_tag="VM692_07520"
     CDS             964..1329
                     /locus_tag="VM692_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6164 family protein"
                     /protein_id="HUQ52055.1"
                     /translation="MSKLLLNLRDVPDDEAEDVRRFLDSGGIGYYETKPSWWGVSAGG
                     IWIGDDGDLPEAKRLMADYQRERHARARAERAEAERAGTAETFVDLLRTQPLRVALAV
                     IGIALVLALMALPVWLLSK"
     gene            1423..2430
                     /locus_tag="VM692_07525"
     CDS             1423..2430
                     /locus_tag="VM692_07525"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ52056.1"
                     /translation="MTNRRRFIEALAVAGAASLLPSQRAVGQPRRAKLNVRGGAIDVH
                     HHFVPPGGATNRPWTPQLSLEQMDEFGIAVAILSMTQNGELLYNGTDKGRAAVRAGNE
                     YGATLMQQYPKRFGLMGGIPMPDLEGSLKEVEYAYDTLKVDAIGIYTNDNHGRWPGDP
                     YFEPLWQELNRRNAIVYMHPLAPLCCSDLKYGAPANMVEYDFDVTRGVVSLVMNGVMF
                     RYPNIRFITVHSGGTVPMLAGRMNDRIPPGAAQYLPNGLYAELRKWYYDIAHASFPWP
                     YAAMKAFMPESQILFGTDYAPEPIESTVDPLPALRLPRAFEQMMLRGNAERLFPRFKV
                     A"
     gene            2551..>3372
                     /locus_tag="VM692_07530"
     CDS             2551..>3372
                     /locus_tag="VM692_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52057.1"
                     /translation="MKRRALISVLVAVCALAGVLAFAVLFIDGERLRTAVIEYVESQG
                     GVELEIERVERTVSLSPRIEVHNVRLRQPEFTDSPLLEIEHAAFDVDLLSFLFGPLTL
                     RNVVVESPMVVLPLGDEGLLYWGPVIADLLERLRRFDWALQSFSIAKLRVEALHTVRD
                     DRVLVTAASIEGTMPHVADLTLRMREVGGDLEAALPLPISGSVAIDELRLAHTGDDSM
                     TVTLEADGRIGSRPLSIRASSLNVVKGDPNARGPVDATLHVDASTLRVSGTASRGA"
BASE COUNT          568 a         1082 c         1133 g          589 t
ORIGIN      
        1 cgcacgacgt tgcaactgcc gggtgccggt ggcggcgtga actggggcgg cgtggcggcg
       61 gatgcgcgca acggcatcgt ctacatgcac acgcacgaca cgtcgctcgt cggttggatc
      121 gaagagcgca agccgggcaa gaactacggc cgcggcgtcg ccggcacgac gattccgtac
      181 gatcgcggca gcgtcaacgg tccggggccg tacttcacct tcagcgccca agcgaagaac
      241 gacgccggcg agtcgttggg ctccttaccg tgctatcggc cgccgtggtc gcgcctcgtg
      301 gcgttcgatg cgaatcgcgg cgagatcctt tggcagagcg tgctcggcgt gaccccgggc
      361 ctgcccgccg ctcggcagct cacgggtggc agcggcaacg cgggcccaac ggccacggcg
      421 ggcggcctcg tgttcgtcgg ctcgacgaac gacaagcgct tccgcgcctt cgacgcgaag
      481 aacggcaaag aggtttggtc ggtgcagctc gacgccgtcg tcaacgcaaa cccgatgacg
      541 taccgcggcc gcgacggtaa gcagtacgtc gcgttcgtcg ccgacgacac gctgcgcgcg
      601 tttgcgctgc cgaagtaacg atgaagaaga ccattttcac ggcgtgtgac gagcactgcc
      661 ggcgcacctt tgcgccgcct tcgcgccgcg ctagtgcccg ctaaaagtcg cgatggatgg
      721 cctaacctgg ttcggtttcc tgtcccttac cggcatgctg atttgctacg cgttggagga
      781 ccgaagccat tggtacatgc tcggcttcac gatcggctgc gtgctcggat cggtctatgg
      841 attcttgcag ggcgcgtggc cgttcggcgt cgtcgaagcg atttggtcgg tcgtcgcgtt
      901 ccaacgttgg cgccgcgtga gggtggcgtg aggcgcacgt gatgcttccg ctatgatcgc
      961 agcatgtcga aactgctgct gaatctgcgc gacgtgccgg acgacgaagc cgaagatgtc
     1021 cgccgcttcc tcgattccgg cggtatcggt tactacgaaa ccaaaccgag ctggtggggc
     1081 gtctccgccg gaggcatctg gatcggcgat gacggcgacc tgccggaggc gaagcgccta
     1141 atggcggact atcagcgcga gcgccacgct cgcgcacgcg ccgaacgcgc cgaggcggag
     1201 cgcgccggga ccgcggaaac gtttgtcgac ctgctgcgca cgcaaccgtt gcgcgtcgcc
     1261 ctggccgtta tcgggatcgc cctcgtgctc gcgctcatgg ccctaccggt gtggctgctg
     1321 tcgaaatagg gagagcggca gtctagcgct gctacgtttc ggccacgcgc cggcgtacga
     1381 tcaacgcgca accatcaagg gcggttagcc gcgcaatctt ccatgacgaa cagacgccgg
     1441 ttcatcgaag cgctcgcagt ggccggcgcg gcatcgctgc ttccgtcgca gcgcgcggtc
     1501 ggccagccgc gtcgcgcgaa gctaaacgtc cgcggcggcg ccatcgacgt gcaccaccac
     1561 ttcgtgccgc ccggtggcgc gaccaacagg ccgtggacgc cgcaactgtc gctcgagcaa
     1621 atggacgagt tcgggatcgc cgtcgcgatt ctctcgatga cgcagaacgg cgagctgctc
     1681 tacaacggga ccgacaaagg gcgagcggcc gtgcgggccg gtaacgagta cggcgccacg
     1741 ttgatgcagc aatacccgaa gcggttcggc ctcatgggcg gtattccgat gccggacctc
     1801 gaaggctcgc tgaaagaagt cgagtacgcg tacgacacct tgaaggtcga cgcgatcggc
     1861 atctacacca acgacaacca tggccgctgg cccggcgacc cgtacttcga gccgctgtgg
     1921 caagagctga accggcgcaa cgcgatcgtc tacatgcatc cgctcgcgcc cctgtgctgc
     1981 agcgatttga aatacggcgc tccagccaac atggtggagt acgacttcga tgtgacgcgc
     2041 ggcgtcgtca gcctcgtgat gaacggcgtg atgtttcgct atccgaacat ccgcttcatc
     2101 accgtgcatt ccggcggcac ggtgccgatg ctcgcgggcc gcatgaacga ccgcatcccg
     2161 ccgggcgccg cgcagtacct gccgaacggc ctctatgccg agctgcgcaa atggtattac
     2221 gacatcgcgc acgcgagctt tccgtggccg tacgcggcca tgaaggcatt catgccggag
     2281 tcgcagatct tgttcgggac cgactatgcg ccggagccca tcgagtccac ggtggacccg
     2341 ttgccggcgc tcaggctacc gcgcgcgttc gagcaaatga tgttgcgcgg caacgccgag
     2401 cgcttgttcc cacgcttcaa agtcgcgtag ccccgacgca cgctcggtag ggtcgagcgc
     2461 tctgcggagg cgcgtcgttt accgcgcgct ttggacgcgt atggcacgga gcgtgcacgt
     2521 gctgggccgt ccactcaaac gagagtgcgg gtgaaacgtc gagctttgat cagcgtgctc
     2581 gtcgcggtct gcgcgttggc cggcgtcctc gcgtttgcag tgctgttcat cgacggcgag
     2641 cgcctacgca ctgcggtcat cgagtacgtc gagagccagg gcggtgtgga gctcgagatc
     2701 gagcgcgtgg agcgcaccgt gagcctgtcg ccacgaatcg aagtacacaa cgtccgcctg
     2761 cgtcagcccg agttcacgga cagtccgttg ctcgaaatcg agcacgctgc cttcgatgtc
     2821 gatctgctgt cgttcctctt cgggccgctg acgctgcgta acgtcgtagt cgagtcgccg
     2881 atggtcgtgc ttcccctcgg cgacgaaggg ctgctgtatt gggggcccgt cattgcggat
     2941 ctgctggagc gccttcgccg cttcgattgg gcgctgcaga gtttcagcat cgcgaagctg
     3001 agagtcgaag cattacatac ggtacgcgac gaccgcgtgc tcgtgactgc ggcttcgatc
     3061 gaaggcacga tgccgcacgt ggcggatctc acgctgcgca tgcgcgaggt cggtggtgac
     3121 ctcgaggcgg cgttgccgct gccgatcagc ggcagcgtgg ccatagacga gctgaggctc
     3181 gcgcacaccg gcgacgactc gatgaccgtg acgctcgaag ctgacggtcg catcggttcg
     3241 cgcccgctca gcatccgagc gagcagcctc aacgtcgtga agggtgatcc gaacgcgcgc
     3301 ggccccgtcg acgcgacgct gcatgtcgat gcgtcgacac tgcgcgtgag cgggacggcg
     3361 agccggggcg cc
//
LOCUS       DATLEE010000350         2362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9336644, whole genome shotgun sequence.
ACCESSION   DATLEE010000350 DATLEE010000000
VERSION     DATLEE010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9336644"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VM692_07535"
     CDS             complement(<1..832)
                     /locus_tag="VM692_07535"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HUQ52058.1"
                     /translation="MTAERLLHALADGRTHSGEELARAFGITRAAVWKQVGKLADWGL
                     TVEAAPGAGYRLPRRLDLLDSDALRAALTPEVAARVAKLEVFTELGSTNRHLLATPPR
                     AGELDVCIAEYQTAGRGRRGRRWSVPLGGGICLSVGWQFADMPADLAALTLAVGVVAR
                     RVLQRVAGLEIELKWPNDLVFDDRKLGGILLELAGEAQGGAHVVAGIGLNVALPPELL
                     PSLSDWPRGAIDIATALGRGPPPRAALAGALINELAELFEDYPRHGFAAYRSEWRAGD
                     Y"
     gene            complement(840..1490)
                     /gene="plsY"
                     /locus_tag="VM692_07540"
     CDS             complement(840..1490)
                     /gene="plsY"
                     /locus_tag="VM692_07540"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HUQ52059.1"
                     /translation="MIELGLKVTLAYLLGSVLGSLLVGFFYGGVDIRKVGSGNAGGTN
                     ALRTQGKAFALWVMVIDIGKGILAAAVIPPLVLPGVGIDPAVDRSLVLYGAAFAAILG
                     HVFPVWFGFRGGKGGATAAGLVCYLAPIGGLVMLVAWVLIVFLTGFVGLATITAALIA
                     VLVIGASALPEQSGLFLFACATAALVIYAHRGNIRRMLDGTESRFASPAARWLRRG"
     gene            complement(1544..>2362)
                     /locus_tag="VM692_07545"
     CDS             complement(1544..>2362)
                     /locus_tag="VM692_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUQ52060.1"
                     /translation="VVGATAFLQRMGELLTRVYRMPGRFKQSLADSSFDAWDVLYRPE
                     PHHPNTTISYYTKGAMVALALDLKLRLETAGRSSLDDVVRELWRRYGALGVGVPEDGF
                     EQLAVEVSGLDLTEFFASAVRGTEDPPLKELLAEFGVALELRAQTGADDRGGTPRAAN
                     GELLTLGVAIREREHGVELTSVLDGGAAERAGLNPGDVLIAVDRLRVTGRNLARRLAR
                     FENGERVTASVFRGDELLEVGIVLKAAPLDTCYLVTRDQAEPKALERRRAWLGE"
BASE COUNT          401 a          848 c          797 g          316 t
ORIGIN      
        1 agtagtcgcc cgcgcgccac tcgctgcgat aggcggcgaa accgtgacgc ggatagtcct
       61 cgaacagctc ggccagctcg ttgatcagcg cgcccgcgag cgcggcgcgc ggcggcggcc
      121 cgcggccgag cgccgtcgcg atatcgattg caccgcgcgg ccagtcgctc aacgagggca
      181 gcaactcggg aggcaacgcg acgttgagcc cgatgccggc cacgacatgc gcgccgccct
      241 gcgcctcgcc ggccagctcg agcagaatgc cgccgagctt gcggtcgtcg aagacgagat
      301 cgttcggcca cttgagctcg atctcgaggc ctgcgacgcg ctgcaacacg cggcgcgcca
      361 cgacgccgac ggcgagcgtc agtgcggcca gatcggccgg catgtccgca aattgccaac
      421 ccaccgacaa gcagatgccg ccgccgagcg gcacgctcca gcggcggccg cggcggccgc
      481 ggccggcggt ctgatactcc gcgatgcaga cgtcgagctc gccggcccgc ggcggcgtcg
      541 ccagcagatg gcgattcgtc gagccgagct ccgtgaacac ctcgagcttc gcgactcgcg
      601 cggcgacctc gggcgtgagc gctgcacgca gcgcgtcgct gtcgagcagg tcgagccggc
      661 gcggcaaacg ataaccggcg cccggtgcgg cctcgaccgt gagcccccaa tcggcgagct
      721 taccgacctg tttccaaacg gctgcacgcg tgatgccgaa cgcgcgcgcg agctcctcgc
      781 ccgagtgcgt tcggccgtcg gccaaggcgt gcagcaagcg ttcggccgtc acgcgttggc
      841 taaccgcggc gcagccaacg ggccgcgggg ctcgcgaacc gcgactccgt gccgtcgagc
      901 attcgccgaa tgttgccgcg atgcgcgtag atcaccaacg cggccgttgc gcaggcgaac
      961 agaaacaagc cgctctgctc cggtagcgct gacgcgccga tgaccaacac ggcgatcagc
     1021 gcggcggtga tcgtcgcgag cccgacgaac cccgtcagaa acacgatcaa cacccacgcc
     1081 acgagcatga cgaggccgcc gatgggtgcg aggtagcaaa cgagacccgc ggccgtcgcg
     1141 ccgcctttac cgccgcggaa accgaaccac acgggaaaca catggccgag tatcgcggcg
     1201 aatgcggcgc cgtagagcac gagcgagcga tcgacggcgg gatcgatgcc gacgcccggc
     1261 agcacgagcg gcggaatcac ggcggccgcc aagattcctt tgccgatgtc gatgaccatg
     1321 acccagagcg cgaacgcctt gccttgcgtg cgcagcgcgt tggtgccgcc cgcgttgccg
     1381 ctgccgactt tgcggatgtc cacgcccccg tagaagaacc cgacgagcag gctgcccagc
     1441 acggagccga gcaagtacgc caacgtgact ttgagtccca gctcgatcat gcgctgccgt
     1501 tcccccgaat cactcgcagc tgtcgaggca tgttacggcg cagtcattcg ccgagccacg
     1561 cccgccgccg ctcgagcgcc ttcggctcgg cttggtcgcg tgtgacgaga tagcaagtgt
     1621 cgagaggcgc cgctttcagc acgatgccga cctcgagcag ctcgtcgccg cggaacacgc
     1681 tggccgtgac tcgctcgccg ttctcgaagc gcgcgagccg gcgcgcgagg ttgcgacccg
     1741 tgacgcgcag gcgatcgacg gcgatcagca cgtcgccggg attcaggccg gcgcgttccg
     1801 cggcgccgcc gtcgaggacg ctcgtgagct cgacgccatg ctcgcgctcg cgaatcgcaa
     1861 caccgagcgt cagcagctcc ccgttcgcgg cgcgcggcgt gccgccgcga tcgtcggcgc
     1921 ccgtctgcgc gcgcagctcg agggcgacgc cgaactcggc gagcagctcc ttcagcggcg
     1981 ggtcttcggt gccgcgcacg gccgacgcaa agaattccgt gaggtcgagg ccgctgactt
     2041 caacagcgag ctgctcgaac ccgtcctcgg gcacgccgac gccgagcgcg ccgtaacggc
     2101 gccacagctc gcggacgacg tcgtcgagcg agcttcgccc agcggtctcg agcctcagct
     2161 tgagatcgag cgcgagcgcg accatcgcgc ccttcgtgta gtaactgatc gtcgtgttcg
     2221 gatgatgcgg ctcgggacga tacagcacgt cccacgcgtc gaagctcgag tccgcgagac
     2281 tttgcttgaa ccggcccggc atgcgataga cgcgcgtcag cagctcgccc atgcgttgca
     2341 agaacgcggt tgcgccgacg ac
//
LOCUS       DATLEE010000351         3320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5614956, whole genome shotgun sequence.
ACCESSION   DATLEE010000351 DATLEE010000000
VERSION     DATLEE010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5614956"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VM692_07550"
     CDS             <1..528
                     /locus_tag="VM692_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUQ52061.1"
                     /translation="AVAIETPFDTQIATLAAQLDGTRLFYGSPQQRAAMEAKVDATER
                     LNDEASVTARARRGAFNVSASGAANFLGERELVDDVASGRVDVATVPAAELPAAVAAL
                     PPEEQRALLADTAQKREELQRQIASLAAERDAFIKKEIEDAGGAAASLDQQLYDAVRE
                     QAAPLGLDYKDGPRF"
     gene            643..1011
                     /locus_tag="VM692_07555"
     CDS             643..1011
                     /locus_tag="VM692_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52062.1"
                     /translation="MSRVSLCLVLLTSSLAAGCAGSRATIAGAEATPTALPARETGSP
                     DATAAALEPAKVVDGNQLAQRSADTLLCRDLLVFGSNMMRKTCGTAAQWKVYELREAE
                     AAAETVRRMQQGRQVSSRRR"
     gene            complement(1008..2789)
                     /locus_tag="VM692_07560"
     CDS             complement(1008..2789)
                     /locus_tag="VM692_07560"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ52063.1"
                     /translation="MENASVDREFALALELHRAGRLAEAEQAYRRILQRAPQHSDALH
                     LLGVIALQAGQLESASTLVERAVALRPDGAIYRNSLGQILEQLGRNDDAVRAYEAALA
                     LEPTYAEACNSLGYLLQRRDRLAEAEVYFRRAIALDARYAEPNTNLGNLLKDRGELDE
                     AIAAYRRAIELRPDLSLLHSNLLLTLHYHRDYGPAELAREHRLWAERHVAPLRSARRP
                     HDNDRDAERRLRVGYVSPDFREHAVARFLLHAFERHDRAQVEVFAYSDDVRGDAVTDR
                     IRAHVDQWRDVQRLRDDELAAAIRADGIDILVDLATHSARNRLLVFARKPAPVQVTYL
                     AYCSTTGVDAIDYRLTDWWLDPPEMDLSHYTERSVRLPGCYWCYSAPPLPAHAQPATQ
                     RAEGPPTFGCLNNFAKVTGATLALWAELLQQVPEARLVLYARGESHRERVRAAFRQAG
                     LAASRVEFVGWQSLQEYLETYRRIDVALDPFPYTGGTTTCDALWMGVPVVSMTGTTAV
                     SRGGSTLLANAGLGECVVATEAQYVALAAGLLRDRARLATLRAELRDRLQRSPVMDAA
                     GFTRGLEAAYREMWRAWCAAASARASG"
     gene            complement(2833..>3320)
                     /locus_tag="VM692_07565"
     CDS             complement(2833..>3320)
                     /locus_tag="VM692_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52064.1"
                     /translation="SPGERLDCYEKQVARATAAAPASQPPAAAQSERPVAAPSQRPAA
                     PAAPTAAARNERPVAAPSEPPAARPRDRAVRTEEARVPNGSTESNGKVTAGISALRET
                     IPNAWLITLDNGQVWRQDYPEPYPLKVGQRVEIATSGRLSGVRLTAAGLRGSIQVERV
                     R"
BASE COUNT          523 a         1136 c         1163 g          498 t
ORIGIN      
        1 gccgtcgcca tcgagacgcc gttcgatacg caaatcgcga cgcttgcggc gcagctggac
       61 ggcactcgcc tgttctacgg ctcgccgcag caacgagccg cgatggaagc gaaggtcgac
      121 gcgacggaga ggctcaacga cgaagcttcc gtgacggcac gcgcgcgtcg cggggcgttc
      181 aacgtcagcg ccagcggcgc ggccaacttt ttgggcgagc gcgagctcgt cgacgacgtt
      241 gccagcgggc gcgtcgacgt ggccacggtg ccggccgcgg agctaccggc ggccgtggcg
      301 gctctgccgc ccgaagagca gcgcgcgttg ctcgccgata cggcgcagaa gcgcgaggag
      361 ctgcagcggc agatcgcgtc gctggccgca gagcgcgacg cgttcatcaa gaaggagatc
      421 gaagacgcgg gcggcgccgc agcatcgctc gatcagcagc tctacgacgc cgtgcgagag
      481 caggccgcgc cgctcggctt ggactataag gacgggccgc gtttctaacg gcacaggaca
      541 ggcttcgcgg gggcggattc acggtccgcc cccgcggcgg gtggacggcg tgaaccttgc
      601 aggagtagcg tggtactcaa cccgccggac caggagaacg tcatgagccg ggtaagcctt
      661 tgcctcgtgt tgctgacctc ctcgctcgcg gctggctgcg ccggcagccg cgcgacgatc
      721 gccggcgccg aagcgacgcc aaccgcgctc cccgcgcgcg aaaccggctc acccgacgct
      781 acggctgcgg ccctcgaacc ggccaaggtc gtcgacggca atcagctcgc gcagcgctcg
      841 gccgacacgc tgctctgccg cgacctgttg gttttcgggt cgaacatgat gcgcaagacg
      901 tgcggcaccg cagcgcagtg gaaggtatac gagctgcgcg aagccgaggc tgccgccgaa
      961 accgtacgcc gcatgcagca aggccgccag gtcagctcgc gccggcgcta gccgctagcg
     1021 cgtgctgacg cggcggcgca ccaggctcgc cacatctcgc gataggcggc ctcgaggccg
     1081 cgcgtgaaac cggccgcatc catgacgggc gagcgctgca agcggtcgcg cagctcggcc
     1141 cgcagtgtcg cgagcctcgc gcggtcacgc agcagtccgg cggcgagcgc gacgtactgc
     1201 gcttccgttg cgacgacgca ttcgccgagc ccggcgttcg ccaacaacgt cgagccgccg
     1261 cgcgagacgg cggtggtgcc cgtcatgctg acgacgggca cgcccatcca caacgcatcg
     1321 cacgtcgtcg tgccgcccgt gtacgggaat ggatcgagcg ccacgtcgat gcggcggtaa
     1381 gtctcgaggt actcctgcag gctctgccaa ccgacgaact cgacgcgcga cgccgcgagc
     1441 cccgcttgcc ggaacgccgc gcgcacgcgc tctcgatgac tctcaccgcg cgcgtaaaga
     1501 acgagcctgg cttcgggcac ttgctgcagc agctcggccc acaacgccag cgtcgcaccg
     1561 gtgactttcg cgaaattgtt caagcaaccg aatgtcggcg gaccttcggc gcgttgcgtt
     1621 gcgggttgcg cgtgcgcggg cagcggcggc gcggaatagc accaatagca gccgggcagc
     1681 cgcacggacc gttccgtgta atgcgataga tccatttcag gcgggtcgag ccaccagtcc
     1741 gtcagccgat agtcgatcgc gtcgacgccc gtcgtgctgc agtaggccaa gtaggtgacc
     1801 tgaacgggcg ccggcttgcg cgcgaacacg agcagccggt tgcgcgccga gtgcgtcgcg
     1861 agatcgacga gaatatcgat gccgtcggcg cggatggcgg ctgcgagctc gtcgtcgcgc
     1921 aggcgctgga cgtcgcgcca ttgatcgacg tgcgcgcgga tgcggtccgt gaccgcatcg
     1981 ccgcgcacgt cgtccgagta cgcgaagacc tcgacttgcg cgcggtcgtg acgctcgaac
     2041 gcgtgcagca ggaaccgcgc cacggcgtgc tcgcgaaaat ccggcgatac gtagccgact
     2101 cgcaatcgtc gctcggcgtc gcggtcgttg tcgtgcgggc gccgcgcgga ccgaagcggc
     2161 gcgacgtgcc gctcggccca gagccgatgc tcgcgcgcga gctctgcggg gccatagtcg
     2221 cggtgatagt gcaacgtcag cagcagattg ctgtgcaaca acgacagatc cggccgcagc
     2281 tcgatcgcgc gccgatacgc agcgatcgcc tcgtcgagct cgccgcgatc cttgagcaaa
     2341 ttgccgagat tcgtgttcgg ctccgcgtag cgcgcatcga gcgcgatggc acggcggaaa
     2401 tacacctcgg cctcggccaa gcgatcgcgc cgctgcaata gataacccag gctgttgcag
     2461 gcttcggcgt aggtgggctc gagcgcaagc gcagcctcgt aggctcgaac ggcgtcgtcg
     2521 ttgcgaccca actgctcgag gatctgaccg agactgttgc gatagatcgc gccgtcgggt
     2581 cgcaaagcaa cggctcgttc tacgagcgtc gaagccgatt cgagctgacc cgcttgcagc
     2641 gcaatgacgc ctagcaggtg cagcgcgtcg gagtgctgcg gcgctcgttg caggatccgc
     2701 cggtaggctt gctccgcctc ggccaagcgc ccggctcgat gcagctcgag cgcgagcgcg
     2761 aattcgcgat ctacgcttgc gttctccatc gacttgtcga ctgttcggcc gcggcggcgg
     2821 cccgcgtcta gatcagcgca cccgctcgac ttggatcgag cctcttagcc cggcggccgt
     2881 cagtcgcacg ccgctcaaac ggccgctcgt ggcaatctcc acgcgttggc ccactttcag
     2941 tgggtacggc tcgggatagt cctgacgcca gacctggccg ttgtcgagcg tgatgagcca
     3001 ggcgttgggg atggtttcgc gcagggccga gatgccggcc gtgaccttgc cattgctctc
     3061 agtcgatcca ttcgggacgc gggcctcctc ggtgcgtacg gctcggtcgc gcggcctcgc
     3121 ggccggcggc tcgctcggcg ccgcgacggg ccgctcgttg cgcgccgccg cggtcggcgc
     3181 cgcaggagcc gccggacgct ggctcggcgc agcaacggga cgctcgcttt gcgcggcagc
     3241 gggcggctgg ctcgccggcg cggcggctgt ggcgcgcgcc acttgcttct cgtagcagtc
     3301 gagccgctct ccgggcgacg
//
LOCUS       DATLEE010000352         2604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1892032, whole genome shotgun sequence.
ACCESSION   DATLEE010000352 DATLEE010000000
VERSION     DATLEE010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892032"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..270
                     /locus_tag="VM692_07570"
     CDS             112..270
                     /locus_tag="VM692_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52065.1"
                     /translation="MAASEGIPHEAFMQGAWQAFLTAHPGMREELEDKALRTELKKLR
                     KRGLIATA"
     gene            complement(272..556)
                     /locus_tag="VM692_07575"
     CDS             complement(272..556)
                     /locus_tag="VM692_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52066.1"
                     /translation="MARQSWIDNDSNPDLDAHVSKLEHFTQSLADGVITAAELEKQEN
                     NLVAAMKAAESALDDAQHAKVTKLLAELTAYSVMRTLHDLTKAHVKGLKL"
     gene            complement(559..2367)
                     /locus_tag="VM692_07580"
     CDS             complement(559..2367)
                     /locus_tag="VM692_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUQ52067.1"
                     /translation="MAPEPTAETKTLAGSSDGVTKATQLPAHFPAWAAKLAELYFSGT
                     TSMFVLHGNTFDVVRAGAENDPRWTGLADFLADQLFGRWDLVIHYDLARGLRCAAGTS
                     SKRLVEMVATSNKWIGDLKALPRDPTKGLLALDLMIQKIVMADEDKRIRCAIIIDHVG
                     YVAPSGDNLDLSEQTHLVTLLNWASSPYIKRLNMAFVLIDPRLSSIAERLTSNPHVAQ
                     IEVPLPNVDERTAFLGAQVKATGKDVTTFSDYSVAELGKLTAGIGLTDLEVLVRSAAE
                     GTRRLDRQYFKELKKRLIERQAQGMLEFVEPKWGLDTVVGHEAAKKRLLDDAELLKRG
                     ELDTVPMGYLFCGPVGTGKSFLATCVACSIGIPAVVLKNFRSKYVGETEGNLERVLGV
                     LRSMGPVVVVIDEADAMLGDRDSGGDSGVGGRVFAMIASQMGDTSYRGRIIWMLLTAR
                     PDQLPVDLKRQGRAEVHIPLFYPTDQAELKTMLMVLAKKSGTSLSESDLPDTVPHMGN
                     LSGADIEGIIGRAYRTSLLGGSKTITKAAIEQALAGFMPSTQTLEREAQELAAIIECT
                     DVEFLPETKREKLDKFGGREKLQERLTAIKQILEER"
     gene            complement(2370..>2604)
                     /locus_tag="VM692_07585"
     CDS             complement(2370..>2604)
                     /locus_tag="VM692_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52068.1"
                     /translation="TNRGRARVAADMSAQGIDNIKAEEQLEKSMADDALKDLEVELGM
                     RAPETTPVQQAQKDLGPAEAEAARKELENLEKS"
BASE COUNT          406 a          852 c          849 g          497 t
ORIGIN      
        1 agccggagct ggccgatcag ttagttacga cgtcgtcgcg gaatgcttct attgaaaagc
       61 atgtccaaca atcgagacga cgtttctggc ctcctcaagc gagccgccga cgtggccgcc
      121 tcggagggca ttccgcacga agcattcatg cagggcgcgt ggcaggcatt cctgaccgct
      181 cacccgggca tgcgcgaaga gctcgaagac aaggcgctgc ggacggagct caagaagctc
      241 cgcaagcgcg gcctgatcgc caccgcttag ctcagagctt cagacctttg acgtgcgcct
      301 tcgtcaggtc gtgcagcgtg cgcatgaccg agtaggccgt cagctcggcg aggagcttcg
      361 tgacctttgc gtgctgcgcg tcgtcgagcg cgctctcggc cgccttcatc gccgcgacga
      421 ggttgttctc ttgcttctcg agctcggcgg ccgtgatcac accgtcggcg agcgactgcg
      481 tgaagtgctc gagcttcgag acgtgagcgt cgagatcggg attgctatcg ttgtcgatcc
      541 agctttgacg ggccatggct atctctcctc caggatctgt ttgatcgcgg tgagccgctc
      601 ttgcagcttc tcgcgaccgc caaatttgtc gagcttctcc cgcttggtct ccggcaagaa
      661 ctcgacatcg gtgcactcga tgatcgcagc gagctcttgc gcctcgcgct cgagggtctg
      721 cgtcgatggc atgaagccgg caagcgcctg ctcgatcgcg gccttcgtga tcgtcttgct
      781 gccgccgagc agcgacgtgc ggtacgcgcg gccgatgatg ccctcgatgt cggcgcccga
      841 caggttgccc atgtgcggca cggtgtcggg caagtcggac tccgacagcg aggtgcccga
      901 cttcttcgcg agcaccatga gcatcgtctt cagctcggcc tgatccgttg gatagaacag
      961 cgggatgtgg acctcggcgc ggccctggcg cttgagatcg acgggcagct gatcgggacg
     1021 cgcggtcagg agcatccaga tgatgcggcc gcgatacgag gtgtcgccca tctgcgatgc
     1081 gatcatcgcg aacacgcgac cgccgacgcc ggagtcgcca ccggagtccc gatcgccgag
     1141 catcgcgtcg gcctcgtcga tcacgacgac gaccgggccc atcgagcgca gcacgccgag
     1201 cacgcgctcg aggttgcctt cggtctcgcc gacgtacttg ctgcggaagt tcttcagcac
     1261 gaccgccgga atgccgatcg agcacgcgac acacgtcgcg aggaagctct tgccggtgcc
     1321 gaccgggccg cagaacagat agcccatcgg gacggtgtcg agctcgccgc gcttgaggag
     1381 ctcggcgtcg tcgagcaggc gcttcttcgc cgcctcgtga ccgacgaccg tgtcgaggcc
     1441 ccacttcggc tcgacgaact cgagcatgcc ctgcgcctgg cgttcgatga gccgtttctt
     1501 gagctccttg aagtactggc gatcgagccg gcgcgtgcct tcggccgccg agcgcacgag
     1561 gacctcgagg tctgtcaggc cgatgcccgc ggtcagcttg ccgagctcgg ccacgctgta
     1621 gtcggagaac gtcgtgacgt ccttgcccgt tgccttcacc tgcgcgccga ggaacgcggt
     1681 gcgctcgtcg acgtttggca acggcacttc gatctgcgcg acgtgcggat tggatgtcag
     1741 ccgctccgca atgctcgaca gccgcgggtc gatcagcacg aacgccatgt tgagccgctt
     1801 gatgtacggg ctcgacgccc agttcaacag cgtcacgagg tgcgtctgct cgctgaggtc
     1861 gaggttgtcg ccgctcggtg cgacgtagcc gacgtgatcg atgatgatcg cgcagcggat
     1921 tcgcttgtct tcgtcggcca tcacgatctt ctggatcatg aggtcgagcg cgagcagacc
     1981 cttcgtcggg tcgcgcggca gcgccttgag gtcaccgatc cacttgttgc tggtcgcgac
     2041 catctcgacg agccgcttgc tgctcgtgcc cgccgcgcag cgcaggccgc gtgcgaggtc
     2101 atagtgaatg acgaggtccc agcggccgaa cagctgatcg gcgaggaaat cggcgaggcc
     2161 ggtccaccgc ggatcgttct cggcgcccgc gcgcacgaca tcgaacgtgt tgccgtggag
     2221 cacgaacatc gacgtcgtgc cggagaagta cagctcggcg agcttggcgg cccacgccgg
     2281 aaaatgcgcg ggcagctgcg tcgccttcgt cactccatcc gacgacccgg ccagggtctt
     2341 cgtctcggca gtcggctctg gagccacggt tacgacttct cgaggttctc gagctctttg
     2401 cgggcggcct cggcctcagc cggaccgaga tccttttgcg cctgctggac cggcgtcgtc
     2461 tccggcgcgc gcatgccgag ctcgacttcg agatccttca gcgcgtcgtc ggccatcgac
     2521 ttctcgagct gctcttcggc cttgatgttg tcgatgccct gcgccgacat gtccgcggcg
     2581 acgcgtgccc tgccgcggtt cgta
//
LOCUS       DATLEE010000353         2524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3193973, whole genome shotgun sequence.
ACCESSION   DATLEE010000353 DATLEE010000000
VERSION     DATLEE010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3193973"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /gene="shc"
                     /locus_tag="VM692_07590"
     CDS             complement(<1..1457)
                     /gene="shc"
                     /locus_tag="VM692_07590"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HUQ52069.1"
                     /translation="MAVSAPRALDTPADRVDATIAKAIAWLDRDQTPEGFWVGMLESN
                     YCMEAEWLLAMHFLGYRHPRQQELVATLVNAQRADGSWEAYYDAPQGDINGTVECYAA
                     LRSVGMSPDDEPLVRARRWIFEHGGLRGIRVFTRYWLALIGEWPWNATPNLPPEIIAD
                     PRWLPFNIYNFASWARATLLPLAVLSARRAVRPLPPERRLDELFPEGRDKTDYRLPRR
                     DSWFSWRRLFLVSDRFLHLYQQLGFTPFRDTAINACLGWIVRHQDADGAWGGIQPPWI
                     YSLMALNVEGYELQHPVMAKGLAALDSHWSYERGGTLHIQASESPVWDTLLALLAMQD
                     CDREFTPAMTRALDWVLSNEVRYRGDWTRKVKGVEPSGWAFERANLHYPDIDDTAVAL
                     IVLSRLPRQLIDEPRIRATIDRALAWTLAMQSSNGGWAAFDKDNDRPIITKIPFCDFG
                     EALDPPSVDVTAHVLEALGVLGYDRHHPAVERGVA"
     gene            1590..1973
                     /locus_tag="VM692_07595"
     CDS             1590..1973
                     /locus_tag="VM692_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52070.1"
                     /translation="MPFIRFGAFCALVSLAGCAAVSTVDGERLKLTSDDFRAYVERVF
                     REQNRVADDVAFALESPGAERSELAAEEQRLLTACAGVNELATARRDDLQLGVRRSLG
                     AARTVPQCEAATRSVAESLRQAQEP"
     gene            complement(2048..>2524)
                     /locus_tag="VM692_07600"
     CDS             complement(2048..>2524)
                     /locus_tag="VM692_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HUQ52071.1"
                     /translation="DGFICAVGTGGTLAGVSAFLKHKNPNIVTAAADPLGAAIYSWIK
                     TGELKGQGSSISEGIGQGRVTGNLDGARIDDAFQIPDTEMLPVLYDLVQKEGLLLGGS
                     TGINVAGAIRLARQLGPGKTIVTVLCDSGSRYQSKLFNPEFLRAKDLPLPPWLVQR"
BASE COUNT          427 a          820 c          862 g          415 t
ORIGIN      
        1 aaggcgacgc cgcgttcgac ggcgggatgg tgccggtcgt agcccagcac gcccagcgcc
       61 tcgagcacgt gggcggtgac atcgacgctg ggcggatcga gcgcctcgcc gaagtcgcag
      121 aacggaatct tcgtgatgat cggccgatcg ttgtccttgt cgaacgccgc ccaaccgccg
      181 ttgctcgact gcatcgccag ggtccatgca agcgcgcggt cgatcgtcgc gcggatgcgc
      241 ggctcgtcga tgagttggcg cggcagccga gagagcacga tcaaagcgac cgccgtgtcg
      301 tcgatgtccg ggtaatgcag attggctcgc tcgaacgccc agccgctcgg ctcgacaccc
      361 ttgaccttgc gcgtccagtc gccgcgataa cggacctcgt tggagaggac ccagtcgagc
      421 gcgcgcgtca tcgctggcgt gaactcgcgg tcgcagtctt gcatcgcgag cagcgcgagc
      481 aacgtgtccc aaaccggcga ctcgctggct tggatgtgca gcgtgccgcc gcgttcgtac
      541 gaccagtgtg agtcgagcgc cgcgagcccc ttcgccatga cgggatgctg gagttcgtag
      601 ccctcgacgt tgagcgccat gaggctgtag atccacggcg gttgaatgcc gccccacgcc
      661 ccgtcggcat cttgatggcg cacgatccaa ccgaggcacg cattgatcgc agtgtcgcga
      721 aacggcgtga agccaagctg ctgatagagg tgcaagaagc ggtcggagac gagaaatagc
      781 cggcgccacg agaaccacga atcgcgccgc ggcaagcggt agtccgtctt gtcgcggcct
      841 tcgggaaaca gctcgtcgag tcgccgctcg ggaggcaagg gtctgacggc gcgccgtgcc
      901 gacagcaccg ccaagggcag caacgtggcg cgcgcccagc tcgcgaagtt atagatgttg
      961 aacggcagcc agcgcggatc cgcgatgatt tcgggcggta gattcggcgt tgcgttccac
     1021 ggccactcgc cgatcagcgc cagccagtag cgcgtgaaga cgcgaatgcc gcgtaagccg
     1081 ccgtgctcga agatccagcg gcgcgcgcgc accaagggct cgtcgtcggg gctcatgccg
     1141 acgctgcgca acgccgcgta gcactcgacg gtgccattga tgtcgccttg cggcgcgtcg
     1201 taatacgctt cccacgagcc gtcggcgcgc tgggcgttca ccagcgtcgc aacgagctcc
     1261 tgctgacgcg gatgccggta gccgaggaag tgcatggcga gcagccactc ggcttccatg
     1321 cagtaattcg actcgagcat gccgacccag aagccttccg gggtctggtc tcgatcgagc
     1381 caggcgatcg ccttggcgat cgtggcgtcg actcggtcgg cgggcgtgtc cagggctcga
     1441 ggcgcagaaa cggccataac taacgcttac ccgttgaacg gcggcgcatt atcgcacaac
     1501 agccgtgccg gcccgccgcg cggcctcaca tccttgcgcg gctgggctcg aatggtcgag
     1561 catgcggtaa cgcccgttac gctatcgcca tgccattcat ccgctttggc gcgttttgcg
     1621 cgctcgtctc tctggccggg tgcgcggccg tctcgaccgt cgacggcgag cgcctcaagt
     1681 tgacgtccga cgacttccgc gcttacgtcg aacgggtatt tcgcgagcag aaccgcgtcg
     1741 ccgatgacgt cgctttcgcg ctcgagtcgc cgggcgccga gcgcagtgag ctcgctgccg
     1801 aggagcagcg cctgctcacc gcgtgcgccg gcgtcaacga gctcgcgacg gcgcggcgcg
     1861 acgatttgca gctcggcgtg cggcgcagct taggcgcggc gcgtaccgtg ccgcagtgcg
     1921 aggcggcgac gcgctccgtc gcggaatcat tgcggcaagc tcaagagccg tagacgcgat
     1981 cgccctgtga gggacgaagg tacctcacag aaaagttgcc tgcgaagagg gcagtgagcg
     2041 ctgcgactca gcgctgcacg agccagggcg gcagcggtaa gtccttggcc cggaggaact
     2101 cggggttgaa gagcttcgac tggtagcgcg agcccgagtc gcagagcacg gtcacgatgg
     2161 tcttgcccgg cccgagctga cgcgcaaggc ggatggcgcc cgcgacgttg atgccggtcg
     2221 agccgccgag cagcaagcct tccttctgca cgaggtcgta gaggaccggc agcatctcgg
     2281 tatcgggaat ctggaacgca tcgtcgatac gcgccccgtc gagattgccc gtgactcggc
     2341 cctgaccgat gccctcgctg atcgagctac cttggccttt gagctcgccg gtcttgatcc
     2401 agctgtagat cgcggcgccg agcgggtccg cggcggccgt aacgatgttc gggttcttgt
     2461 gcttcaaaaa cgcgctgacg ccggccagcg tgccgcccgt gccgacggcg cagatgaagc
     2521 cgtc
//
LOCUS       DATLEE010000354         4026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1149635, whole genome shotgun sequence.
ACCESSION   DATLEE010000354 DATLEE010000000
VERSION     DATLEE010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149635"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VM692_07605"
     CDS             complement(<1..667)
                     /locus_tag="VM692_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52072.1"
                     /translation="MNALVEHYLLQLQTFLPAKQRQDIAAELRESIRATVEERERELG
                     RALTHDEVSSVLEGFGHPMLVAGRYLPMQQLIGPDVFPLYWYVLQALLIVLTIIGGLL
                     AGVAVMTEPRAVQAAMQVAARFFWVALEAAAIVTLVFAVLEQANARFSFLEKFDARKL
                     NAGIWGVRAAPLSPIPRADTVFEIATVALLLLWWVDWVVFPNRLFGVTLQMSSKVDAF
                     FYPV"
     gene            complement(688..1017)
                     /locus_tag="VM692_07610"
     CDS             complement(688..1017)
                     /locus_tag="VM692_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUQ52073.1"
                     /translation="MRVSSELIDKLRLELRRGSLTIAVLAQLKSERYGYTLRKALAEL
                     GLEIDEGTLYPLLRRLETQGLLTSEWREEDKREKRFYRLSAEGRTILKQLLDEWRALN
                     GALDNIV"
     gene            complement(1469..2695)
                     /locus_tag="VM692_07615"
     CDS             complement(1469..2695)
                     /locus_tag="VM692_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008234738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ52074.1"
                     /translation="MSAPIVDTGVATEDGRAKRNVAVLVFAHAILGSQLAINIIVAGL
                     AGAFLANDPSLATLPISIVVVGSLLTAPAMSLFMGRYGRRAGFWVAALAGAIAAALCA
                     RALYINSFGLFMAGSVLLGVYQATQGFFRFAAADTASERFKPRAISWVLAGGLLSALL
                     GPEIVRATSGTIASVPYAGAYLAMIALNVLGAAGLWFLDIPTPARAAKAADTGRRLAV
                     IASQPVFLVAVLAAMVGFSSMSLVMTSTPLAMVGHGFTPDHAADVVRWHIVAMFAPSF
                     ATGAVIARFGRLPVIAVGLLLLGVCGAIALAGVDLTHFYLALIALGVGWNFSYIGATS
                     LLGTTHTPAEQAKVQGLNDFLVLGFVAVGSFGSGALLDAFGWDAVQYAMAPALLVALV
                     GVLWLASVQRRKAAGL"
     gene            2782..3369
                     /locus_tag="VM692_07620"
     CDS             2782..3369
                     /locus_tag="VM692_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ52075.1"
                     /translation="MNKTHTILGVATLALLVGVVVNGVPGTAAQGAKALTAQDVLDIR
                     GLIDNYSHLLDNCVNNGNDYANLYTDDATFGVSQEYGKAKIWFRGREQLRRAGGGTDT
                     ACRPQQSQGYAYHLAINPMITATPTGAHAITTLLTITNDTNSRGDIVHWEGGYEDTFE
                     KTPNGWRFKSRVHVWPEVAWTDRVEDMPARKLEDE"
     gene            complement(3377..>4026)
                     /locus_tag="VM692_07625"
     CDS             complement(3377..>4026)
                     /locus_tag="VM692_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52076.1"
                     /translation="ARVRWAVFAALATALVVSSTTLAGSNAAELVPVPAAILWIAGIA
                     ALVAARWRLRDALPAVLERAAQVGLVLTAVYVGALVAASAAARSEVRSVAAQRGIAAE
                     DVMVAPTPANPFRGDVIVMTAEEYYVGRFDWLGAPRVTLDGEPVPRPRGPVYERASLD
                     PNARRFLLWARYPAIEMETLPSGGTRVRFTDVRYRAADRLSGPVVELPPGRARLD"
BASE COUNT          793 a         1370 c         1300 g          563 t
ORIGIN      
        1 gcaccgggta gaagaaggcg tcgaccttcg agctcatttg caacgtgacg ccgaacaggc
       61 ggttcggaaa gacgacccaa tcgacccacc acagcagcaa caaggcgacg gtcgcgatct
      121 cgaacaccgt gtcggcgcgc gggatcggcg acaacggtgc ggcgcggacg ccccagatcc
      181 cggcgttgag cttgcgtgca tcgaacttct cgaggaacga aaaccgcgcg ttggcctgct
      241 cgagcacggc gaacaccaag gtcacgatgg cggccgcctc gagcgccacc caaaagaacc
      301 gcgcggcaac ttgcatcgcc gcttggaccg cgcgcggctc cgtcatcacc gcgactccgg
      361 ccagcagccc gccgatgatc gtgaggacga tcagcaacgc ctgcagcaca taccagtaga
      421 gcgggaacac gtcggggccg atcagctgct gcatcggcag atagcgtccg gcgactagca
      481 tcggatggcc gaagccctcg agcacggagc tcacctcatc gtgcgtgagc gccctcccga
      541 gctcgcgctc gcgctcctcg acggtcgcac ggatgctctc gcgcagctcg gccgcgatgt
      601 cttggcgctg ctttgccggc aaaaatgtct gcagctgcag taggtaatgc tcgactaacg
      661 cattcatatg gtttcctcga ccctcggtca tacgatgttg tcgagtgcgc cgttcagcgc
      721 gcgccactcg tccaagagtt gcttcaggat cgttcggccc tcggcggaga gccgataaaa
      781 gcgcttctca cgcttgtctt cctcgcgcca ttcgctcgtc aacaagccct gcgtctcgag
      841 ccggcgtagc agcggataca gcgtgccctc gtcgatctcg aggccaagct ccgcgagggc
      901 tttgcgcagc gtgtaaccgt aacgctcgga cttgagctgc gcgagcacgg caatcgtcaa
      961 cgacccgcgg cgcaactcga gacggagctt gtcgatgagc tcggatgaca ctcgcatgct
     1021 tcacatactg tgtggcattt actgtatagc acacagtata gagacgcaag cgcgaaaatt
     1081 tcaggaggaa cgtgtctctc tttacggatg accgagctga cctcgagcgt cgtggttgct
     1141 cgggaatgag cccgatgcac gtcaacgaat acttgcccgc cggcaattga atctctttgc
     1201 gctcgccgta gcgcgtgcgt gaggttcggc atgtcggcgt tgattaagcg cttcgcgacg
     1261 agcgacacga tgacctgctg gccctgcacg ccccattcga ggccgcgtcg aacacgagcg
     1321 tgcccgtgct ctgcgccatg gtgggctagg cgaacccgta cgagaggatc aacgcggccg
     1381 cgccggcgaa cgaacgattc atctggtggt cctctgaaat ggcgccgcga gttttagagt
     1441 gcggctcgcc accgggctcg gaaggcggtc acaagccggc ggctttgcgg cgctgcaccg
     1501 aggcgagcca caggacgccc acgagcgcga cgagcaacgc cggcgccatc gcgtactgca
     1561 cggcatccca gccgaacgcg tcgagcagcg cgcccgatcc gaaggagccc acggcgacga
     1621 agccgagcac caagaagtcg ttcaagccct ggaccttcgc ttgctcggcc ggcgtatgcg
     1681 tcgtgccgag caagctcgtc gcgccgatgt aggagaagtt ccagccgacg ccgagcgcga
     1741 tcagcgccaa atagaaatgc gtcaggtcga cgccggcgag cgcgatcgcg ccgcagacgc
     1801 cgagcaatag caggccgacg gcgatgacgg gcaagcggcc gaatcgcgcg atcacggcgc
     1861 ccgtggcgaa gctcggcgcg aacatcgcga cgatgtgcca acgcacgacg tcggcagcgt
     1921 gatccggcgt gaagccgtgt cccaccatcg cgagcggcgt cgacgtcatg acgagactca
     1981 tcgacgagaa gccgaccatc gcggcgagca cggcgacgag gaacaccggc tggctcgcga
     2041 tgacggcgag ccggcggccc gtgtccgcgg ctttcgcagc gcgcgccgga gtcggaatgt
     2101 cgaggaacca caaacccgca gcgccgagca cgttcagcgc gatcatcgcg agataggcac
     2161 ccgcatacgg cacgctcgcg attgtccccg aggtcgcacg cacgatttcc gggccgagca
     2221 gcgcgctcag gagtccgccg gcaagcaccc acgagatcgc cctcggcttg aaccgctcgg
     2281 aggccgtgtc ggccgccgca aagcggaaaa aaccctgcgt cgcctgatag acgccgagca
     2341 acacggaccc ggccatgaac aacccgaagc tgttgatgta caacgcacgc gcacagagcg
     2401 ccgccgcgat cgcgcccgcg agcgccgcaa cccagaagcc cgcgcgccgg ccgtagcggc
     2461 ccatgaacaa cgacatcgca ggcgccgtca gcagtgagcc gacgacgacg atcgagatag
     2521 gcagcgtcgc cagtgacggg tcgttggcca aaaaagctcc ggccagcccc gcgacgatga
     2581 tgttgatcgc gagctgggag cccaggatcg cgtgcgcgaa cacgagcacc gcgacgttgc
     2641 gcttcgctcg gccatcttcg gtcgcgactc cggtatcgac gattggggca ctcacgacaa
     2701 taggtcaccg aggcggcgcg ccaggttaac atacggaccg acggcgaacg gttaggtact
     2761 cgagcacacg gagctcatgg catgaacaaa acacacacga ttctcggcgt cgcaacgctg
     2821 gccttgctcg tcggagtcgt cgtcaacggc gtgcccggca ccgcggcgca gggcgccaag
     2881 gcgctgacgg cgcaagacgt gctcgacatc cgcgggttga tcgacaacta ctcgcatctg
     2941 ctcgacaact gcgtcaacaa tggcaacgac tacgcgaacc tttacaccga cgacgccacg
     3001 ttcggcgtct cgcaagagta cggcaaggcc aagatatggt tccgcggccg cgagcagctg
     3061 cggcgagccg gcggcggcac cgacacggct tgccggccgc agcagtcgca aggctatgcg
     3121 tatcacctcg caatcaatcc catgatcacg gcgacgccga ccggcgcgca cgcgatcacg
     3181 acgctgctga cgatcacgaa cgacacgaac agccgcggcg acatcgtgca ctgggaaggc
     3241 ggctacgagg acacgttcga gaagacgccg aacggctggc gcttcaagtc gcgcgtgcac
     3301 gtctggcccg aagtcgcgtg gaccgatcgc gtcgaggaca tgccggcgcg caagctcgag
     3361 gacgaatgag cgcggtttag tcgagacgag ccctgccggg agggagctcg acgacggggc
     3421 cggaaagccg gtcggccgca cgatagcgaa cgtccgtgaa cctcacgcgc gtgccgccgc
     3481 tcgggagcgt ttccatctcg atggcagggt accgcgccca caggaggaac cgtcgcgcgt
     3541 tcgggtccag cgaggccctc tcgtagacgg gccctcgcgg ccgcggcacg ggctcgccgt
     3601 cgagcgtgac tcgcggcgcg ccgagccagt cgaagcgccc gacgtagtac tcctcggccg
     3661 tcatgacgat cacgtcgccg cggaacgggt tggccggagt gggcgcaacc atcacgtcct
     3721 cggccgcgat gcctcgctgc gcggcgacgg agcgaacctc gctgcgcgct gccgcgctcg
     3781 ccgccacgag cgcaccgacg tagacggccg tcagcacgag gccaacctgc gccgcacgtt
     3841 cgagcactgc cggcagcgca tcccgcagcc gccatcgcgc cgctaccaac gccgcaatgc
     3901 ccgcgatcca caggatcgcc gccggcaccg gcacgagctc cgccgcgttc gatccggcga
     3961 gcgtggtgct cgagacgacc agcgcggtcg ccaacgcggc aaacacggcc cagcgcacgc
     4021 gcgccc
//
LOCUS       DATLEE010000355         3100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_407405, whole genome shotgun sequence.
ACCESSION   DATLEE010000355 DATLEE010000000
VERSION     DATLEE010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407405"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..881
                     /locus_tag="VM692_07630"
     CDS             81..881
                     /locus_tag="VM692_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUQ52077.1"
                     /translation="MQHRQPRAAPPSTAAAFCIALVVIAASNQAAAQTATRYERTDIE
                     YGARLFNNHCIACHGENGDLVPQINLRNGRFPHSPSDRDLGENIKNGIPGTPMVATSY
                     TESEISALVAYVRNIGNIDLSGMQVGDATRGRALFEGKGACASCHRVFGRGPRAAPEL
                     SNIGAVRSAAQLERALLGSEGGVIPINRPVRIVTRDGTVINGRRLNEDRYTVQLVDER
                     ERLLSLDKSTLREYAVLETPVMPSYAQTLSEEERADVLAYLLTLKGLP"
     gene            878..2536
                     /locus_tag="VM692_07635"
     CDS             878..2536
                     /locus_tag="VM692_07635"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HUQ52078.1"
                     /translation="MMLRRLAVLLLAALATGPQYLAAQVTPERLVATQREPQNWLTYS
                     GNYASTRYSELTQIRPANVANLELQWVFQQQSLEKFETSPLVVDSVMYVTEAPNTVVA
                     LDAKTGAAFWRYVHTPSSASRPCCGRVNRGVAIHDGTLFMATIDAELIALDAVTGQPN
                     WRVAVADASLGYAMTLAPLIIDDKVIVGVAGGEYGIRGFIAAYAAGTGEELWKFYTIP
                     GPGEPGHETWEGGGDAWEHGGASVWVTGSYDPELNLTYWGIGNPGPDWNPAQRPGDNL
                     YSNSVVALDADTGKLKWHFQFTPNDDYDFDATQVPVLVDLPIGVSSGKLMLWGNRNGF
                     FYVLDRVTGEFIRGAPFVKVNWARGLDESGRPIETPQPPGATTYPCVSGGTNWYSPSY
                     SPRTELFYVSAWENCGAVMGPQEAEYRPGQRFLGGRPTSPNPGAPGIPGGAAGRGAIN
                     NWTEEMGNGAVLAIDPRTGETRWRFPLTDVTSSGVLTTATDVVFTGSREGHFHALDAR
                     SGRLLWRASLGGQIANGPMTYAVDGKQYVAVAAGSGLYVFALGD"
     gene            complement(2574..>3100)
                     /locus_tag="VM692_07640"
     CDS             complement(2574..>3100)
                     /locus_tag="VM692_07640"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HUQ52079.1"
                     /translation="AGWRPRVPAERRPDLGRVATGRGVALGTHTSSYGAAVAEVEVDT
                     TTGIIAVRQLYGALDAGLVVNPGFVENQISGMLVQAASRMLKEEVTFDAQRVTSLDWV
                     SYPILRFAECPAVTPIVVQRLNEPYSGGGEEVLAPAAAAIANAFADATGVRLREFPLT
                     PPRVLAALQPGAAG"
BASE COUNT          523 a         1063 c         1043 g          471 t
ORIGIN      
        1 gcacgcccgg cctcgtgtgg agctgcagcc ggggcaccgg agcagtgcta ctatcggttg
       61 cgcacgcgcg ggggataaca atgcaacacc gacagccgcg agccgctccg ccctcaaccg
      121 cggcggcctt ttgcatcgct ctcgtagtta tcgctgcgag caaccaggcg gccgcgcaaa
      181 ccgcaactcg ttacgagcgc accgacatcg agtacggcgc gcggctgttc aacaaccact
      241 gcatcgcgtg ccacggcgag aacggcgatc tggtgccgca gatcaatctg cggaacggtc
      301 ggtttccgca ttcgccgagc gaccgagacc tcggcgagaa catcaagaac ggcatacccg
      361 gcacgccgat ggtcgctacg agctacacgg agtcggagat cagcgcgctc gtggcgtacg
      421 tccgcaacat cggcaacatc gacttgagcg ggatgcaagt cggcgacgcc acgcgcggcc
      481 gcgcgctgtt cgaaggcaaa ggcgcgtgcg cgagctgcca ccgtgtgttc gggcgcggcc
      541 cgcgcgccgc gccggaattg agcaacatcg gcgcggtccg ctccgcagcg cagctcgagc
      601 gtgcgttgct cggcagcgag ggtggtgtga tcccgatcaa tcgccccgtg cgcatcgtca
      661 cgcgcgacgg caccgtcatc aacggccggc gcctcaacga ggaccgctac acggttcagc
      721 tcgtcgacga gcgagagcgg ctcctgtcgc tcgacaagag cacgctgcgc gagtacgcgg
      781 tcctcgaaac gccggtcatg ccgtcgtatg cgcaaacgct gagcgaggaa gagcgcgccg
      841 acgtgctcgc gtacctgctg actctgaagg ggttgccatg atgctccgac gactcgcagt
      901 gctcctgctc gctgctctcg ctacgggccc gcagtatctc gccgcgcaag tcacgcccga
      961 gcgcctggtc gctacgcagc gagaaccgca gaattggctg acttactcgg ggaactatgc
     1021 gagcacgcgc tacagcgagc tgacgcagat ccgtcctgcg aacgtagcga acctcgagct
     1081 tcagtgggtg tttcaacagc aatcgctcga gaagttcgag acgtcgccgc tcgtcgtcga
     1141 cagcgtcatg tacgtgaccg aagcgccgaa cacggtcgtt gcgctcgacg cgaagaccgg
     1201 cgccgcgttc tggcgctatg tgcatacgcc ctcctccgca tcacgcccct gctgcggccg
     1261 cgtgaaccgc ggcgtggcga tacacgacgg taccttgttc atggcgacga ttgacgcgga
     1321 gctgatagca ctcgatgccg ttacgggcca gccgaactgg cgcgttgcgg tcgccgatgc
     1381 aagcctcggc tacgcgatga cgctcgcgcc gctgatcatc gacgacaaag tcatcgtcgg
     1441 cgtggccggc ggcgagtacg gcatccgcgg cttcatcgcc gcctacgcgg ccggcaccgg
     1501 cgaggagctc tggaagtttt acacgatccc cggtcccggg gagcccggcc acgagacctg
     1561 ggagggcggc ggcgatgctt gggagcacgg cggagcgtcc gtctgggtca cgggctcgta
     1621 cgatccggag ctcaatctga cgtactgggg catcggcaat ccggggcccg actggaaccc
     1681 cgcccagcgc cccggcgaca acctctattc gaacagtgtc gtcgcgctcg atgccgacac
     1741 gggcaagctc aagtggcatt tccaattcac gccgaacgac gactacgact tcgacgccac
     1801 gcaggttccc gtgctcgtcg atctgccgat cggcgtgagc agcggcaagc tcatgctctg
     1861 gggcaatcgc aacggtttct tttacgtcct cgatcgcgtc acgggcgagt tcattcgcgg
     1921 cgcgccgttc gtcaaggtca actgggcgcg cgggctcgac gaaagcgggc ggccgatcga
     1981 gacgccgcaa ccgccgggcg cgacgaccta tccgtgcgtc agcggcggca cgaactggta
     2041 ctcgccgtcg tatagcccgc gcacggagct gttctacgtt tcggcctggg agaactgcgg
     2101 cgccgtcatg ggcccgcagg aagccgagta tcggccgggg cagcgatttc tcggcggccg
     2161 cccgacgagc ccgaaccccg gcgcgcccgg cattccgggc ggcgcggccg gtcgcggcgc
     2221 gatcaacaac tggacggaag agatgggcaa cggcgccgtg ctggcgatcg atccgcgaac
     2281 cggcgagacc cgctggcgat tcccgctgac cgacgtcacc tcgagcggcg tgctgaccac
     2341 cgcgacggac gtcgttttta cgggcagccg cgaagggcac ttccacgcgc tcgatgcgcg
     2401 cagcggcagg cttctgtggc gcgcgagcct cggcggtcag atcgcgaacg gcccgatgac
     2461 ctatgccgtc gacggcaagc agtacgtcgc cgtcgcggcg ggcagcggac tgtacgtgtt
     2521 cgcgctcgga gactagtcgc aggcgtggcg tcgcgttttg cgcacgagca ccgtcaaccg
     2581 gcagctcctg gctgcagcgc cgcgagcacg cgcggcggcg tcagcggaaa ctcgcggagc
     2641 cgcacacccg tcgcgtctgc gaacgcgttc gcgatcgcgg ccgccgccgg cgcgagcacc
     2701 tcttcgccgc cgccgctgta gggctcgttc agccgctgca cgacgatcgg cgtgacggcc
     2761 gggcactcgg caaagcgcag aatcggatag ctgacccaat cgaggctcgt cacgcgctgc
     2821 gcgtcgaacg tgacctcttc cttcagcatg cggctcgcgg cctgaacgag catccccgag
     2881 atctggttct cgacgaaacc cggattgacg acgaggccgg cgtcgagcgc gccgtagagc
     2941 tgccgcacgg cgatgatgcc cgtcgtcgtg tcgacctcga cttcggccac ggccgcgccg
     3001 taagacgacg tgtgcgtgcc gagcgcgacg ccgcgcccgg ttgcgacgcg gccgaggtcg
     3061 ggccggcgtt ccgccggcac acggggccgc cacccggctg
//
LOCUS       DATLEE010000356         5249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1896226, whole genome shotgun sequence.
ACCESSION   DATLEE010000356 DATLEE010000000
VERSION     DATLEE010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896226"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VM692_07645"
     CDS             complement(<1..550)
                     /locus_tag="VM692_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HUQ52080.1"
                     /translation="MDIPRPENKKRKRIRQLAIGGSTAVVLVLVTIGLARLEPAAPSV
                     DADTQYIGDVSRGEMLRQVRGPGTLVPREIRWIAAQSAGRVDRVVTLAGTVVAPDTIL
                     VQLSNPDLMSESEKAGYALEAAKAEHAELELKLRSTELDQKAALAQARAQYEGARLQA
                     EAERDAGVVAVLTVRRSELTAEQ"
     gene            718..1086
                     /locus_tag="VM692_07650"
     CDS             718..1086
                     /locus_tag="VM692_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ52081.1"
                     /translation="MARLNHLALIVSDWQRSRDWYVRCLALKVEFEVPERRTVALQDE
                     SGLTLFLSEQPEGFVAASCTLAFEVDDVEATHRELAARGVTFEKAPQKLFWGYGAELR
                     DPDGYLVYLWDEKSMREKGG"
     gene            1126..2418
                     /gene="tyrS"
                     /locus_tag="VM692_07655"
     CDS             1126..2418
                     /gene="tyrS"
                     /locus_tag="VM692_07655"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HUQ52082.1"
                     /translation="MQILDELAARGLVADVTDRDALTKLLGTERLTFYVGYDPTSSSL
                     HVGNLVPVIMQARLQRAGHRPIVLVGGATGMVGDPSGKSAERNLLGDEELAANSAGIR
                     AQLSRFLDFGSGPTGAVLANNADWTRGMSYLEFLRDVGKHLTVNYMMAKDSVRARLEG
                     ETGISYTEFSYMLLQAWDFVHLSRAYGCRLQVGGSDQYGNITAGCELSRKMGGPQLFG
                     LTAPLLLDSSGQKMGKTSTGERVWLDAERTSPYAFYQYFFNVSDDDAPRLLRLFSLRP
                     LAEIDEIARSHAADPSQRIGQRELAQTMTTWVHGADETARVEEASRIMFGGSLDGVHE
                     RTLELLAKVVPVVDVDPAELASGIGVVELLSRTVAESKGAARRLLQQGGAYINNVRVT
                     DGDRKVSTADLATPTMLVVRGGRKDYRLVRAAVQRLPK"
     gene            2628..2813
                     /locus_tag="VM692_07660"
     CDS             2628..2813
                     /locus_tag="VM692_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52083.1"
                     /translation="MLTLHEKAAALAAEHDVDSTRELEQLAELVRLAQLAMDRFQTFT
                     IELKVLVFELGQARDSH"
     gene            complement(2829..4580)
                     /locus_tag="VM692_07665"
     CDS             complement(2829..4580)
                     /locus_tag="VM692_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52084.1"
                     /translation="MVPISETTQNVARDPVGAPPPSAVEVRAALERILASRCFEQAAR
                     SSKFLRFVVEQTLAGQGDRLKGYTIAIEVFGRPPDFDAQSDPLVRVEAGRLRRRLTEY
                     YADEGRNDAVRIELPRGSYTVTSAYHPAPAEVLLGPMPPATPDANDSARNRRRWRRVR
                     SLLIAAVVLAGLGVVLLQHFELSRLTGGPALAVQGRARAGGKPPIVVLPFEDLGGDEP
                     AISRLAATLREELLLLLDDPHLFVVATQGEAGQAAADSGAYVLSGSVRVTAGQVRVTA
                     RLLAPGTGTQLWSSAYDEPLASLRDPVGQRSLARRLAAVAEAYGPIFESELQRVGAVA
                     ASALTLSDCLFKYYQYRRVLDAARHADARACYEAATVREPGSAEAWAGLSLLGTEQYL
                     FGYGADAASVDRAREAARRAMDIDGDSLHANLALAAAQFVAGADFRPVAERVLTRWPD
                     SGEAQAFLGSFFTVTGDTARGRELLDRALASTERPPSGYHSTRALVALREGNFDEALT
                     MALRIDAPDWPLGQLVVAAIASQAGRADLAARAKARAIKLNPSLETALPEVVKRWRVE
                     PVLAELVQRGFAEAGGR"
     gene            complement(4643..>5249)
                     /locus_tag="VM692_07670"
     CDS             complement(4643..>5249)
                     /locus_tag="VM692_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52085.1"
                     /translation="TNPPPDAPPPLKANFQAEWDKRRAAQREADARGEPPATNVTHCI
                     PDGVPSMMNGPFPFEIVQNATQINIAQEAYSQIRRIYLDKPQLTLDDIELGFYGRSVG
                     KWEGDTLVVDTIGIKDYVRFRDVPHTRDMHITERFKLVTPEILWDEITIEDPAVLEKP
                     WTVTFAYKRMPDYEIIEYVCEDNREYADPNTGATRLRTGGE"
BASE COUNT          844 a         1757 c         1762 g          886 t
ORIGIN      
        1 actgctcggc agtcagctcc gagcggcgga cggtcagcac ggcgacgacg ccggcgtccc
       61 gttcggcctc ggcttgcagc cgcgcgccct cgtactgcgc gcgcgcctgc gcgagcgccg
      121 ccttttggtc gagctcggtg ctgcggagct tgagctcgag ctcggcgtgc tcggccttcg
      181 cggcctcgag cgcgtagccg gctttctcgc tctcgctcat gagatcggga ttcgacagct
      241 gcacgagaat cgtgtcgggc gcgacgacgg tgccggccag cgtgacgacg cggtcgacgc
      301 ggcccgccga ctgcgcggcg atccagcgaa tctcgcgtgg cacgagcgtg ccgggaccgc
      361 gcacctgccg cagcatttca ccgcgactaa cgtcgccgat gtactgcgtg tcggcgtcga
      421 ccgagggcgc cgcgggctcg agccgcgcga ggccgatcgt gacgagcacg agcaccacgg
      481 ccgtactgcc gccaatcgcg agttggcgga tccgtttgcg ctttttgttt tctgggcgtg
      541 ggatgtccat ttgccggctc ttagcaacga atgtgccacg cgagtgtacg ccttttaagt
      601 cattggatta gatgtagaaa ggctaccggc tcggcgttgc ggggccgcgt gctccgtccc
      661 gcacgcgcgt ccccacgtcc cgaaagcggg acaagcggcg cgccgctaga atgccgcatg
      721 gctcggctca accatctcgc cttgatcgtc tcggactggc agcgctcgcg cgactggtac
      781 gtgcggtgcc tcgcgttgaa ggtcgagttc gaagtacccg agcgcaggac cgtcgccttg
      841 caggacgaga gcggtctcac gttgttcctc tccgagcagc ccgaaggctt cgtcgccgca
      901 agctgcacgc tcgcgttcga ggtcgacgac gtcgaggcga cgcaccgcga gctcgcagcg
      961 cgcggcgtca cgttcgagaa agcgccgcag aaacttttct ggggctacgg tgccgagctg
     1021 cgcgatcccg acggctatct cgtctatcta tgggacgaaa agtcgatgcg cgagaaaggc
     1081 ggttgagccg cgccgcggcg attggcgatc gggtaatctt ccgcaatgca aattctcgat
     1141 gaactggccg cccgcggcct cgtcgcggac gtcacggacc gcgacgctct gacgaagctc
     1201 ctcggcacgg agcgcctgac cttctacgtc ggctacgatc cgacgtcctc cagcttgcac
     1261 gtcggcaatc tggtgccggt catcatgcag gcacggctgc aacgcgccgg tcacaggccc
     1321 atcgtcctcg tcggcggcgc gacgggcatg gtcggcgacc cgtcgggcaa gagcgccgag
     1381 cgcaacttgc tcggcgacga ggagcttgcg gccaactcgg ccggtattcg cgcgcaactg
     1441 tcgcgctttc tcgacttcgg ctcgggaccg acgggagccg tgctcgccaa caacgcggat
     1501 tggacgcgcg gcatgagcta cctcgagttc ctgcgtgacg tcggcaagca tctgaccgtc
     1561 aactacatga tggccaagga ctccgtgcgc gcgcgtctcg agggcgagac cggcatcagc
     1621 tataccgagt tctcgtacat gctgctgcag gcctgggact tcgtgcacct gtcgcgtgcg
     1681 tacggctgcc ggctgcaggt cggcggctcg gaccagtacg gcaacatcac ggccgggtgc
     1741 gagctgtcgc gcaagatggg cgggccgcag ctgttcggct tgacggcgcc gctgctgctc
     1801 gactcctcgg ggcagaagat gggcaagacc tcgacgggcg agcgcgtctg gctcgacgct
     1861 gaacgcacgt cgccgtacgc gttttaccag tacttcttca acgtcagcga cgacgatgcg
     1921 ccgcggctgt tgcgcctgtt ctcgttgcgg ccgctcgcgg agatcgacga gattgcccgc
     1981 agccacgctg ccgaccctag ccagcggatc ggccagcgcg agctcgcgca gacgatgacg
     2041 acttgggttc atggcgccga tgaaaccgcg cgcgtcgagg aagcaagcag gatcatgttc
     2101 ggcggcagct tggacggtgt gcacgaacgg acgctcgagc tgctggccaa ggtcgtcccc
     2161 gtggtcgacg tcgatccggc cgagcttgcg agcgggattg gagtcgtcga gctactgagc
     2221 cgcaccgtgg cagagtcgaa aggcgccgcc cgccggctgc tgcagcaagg cggtgcctac
     2281 atcaacaacg tgcgtgtcac ggacggcgac cggaaggtca gcacggccga cctggcgact
     2341 ccgacgatgc tcgtcgtacg cggcgggcgc aaggactacc ggctcgtccg agccgcggtt
     2401 cagcgccttc cgaagtagac gtaacggtag ttttgccggc gctcgtcctc gggcgagcac
     2461 ggctttccgc acgactaccg tgtgacacgt cacgaggcgc ggcgcgggtt ctatcgtggt
     2521 ggtcactcta cccgaccgcc aaacctatga acccgcaaag accctactcg cggcgcgctt
     2581 tagtcgcagc gctcgccgac cggcttcgcc acggaccgct gcaagcgatg ctgacgcttc
     2641 atgagaaggc ggcagcgtta gcggccgagc acgacgtcga ttcgactcgc gagctcgagc
     2701 agctcgcgga gctcgtgcgc ctcgctcagc tcgcgatgga tcgttttcaa acgttcacga
     2761 tcgagctcaa ggtgctcgtg ttcgagctcg ggcaggcgcg cgactcgcac tgagcccgca
     2821 gcacttcgtc aacggccacc ggcctctgca aagccgcgct gaaccagctc ggcgagcacc
     2881 ggctcgactc gccagcgctt cacgacttcc ggaagcgccg tttcgagaga cggattgagc
     2941 ttgatcgcac gtgccttggc ccgagccgca aggtccgcgc gtccggcctg cgaggctatc
     3001 gctgcaacga cgagctgccc caacggccaa tccggtgcat cgatgcgcag cgccatcgtg
     3061 agagcttcgt cgaaattccc ttcgcgcaac gctacgagcg cgcgcgtgga gtgatagccg
     3121 ctcggagggc gctccgtcga tgcaagcgcg cgatcgagca actcgcggcc gcgcgcggtg
     3181 tcgcccgtta cggtgaagaa cgacccgagg aatgcttgag cctcaccgct gtcgggccaa
     3241 cgggtcaata cccgttcggc gactgggcgg aaatccgcgc ccgcgacgaa ttgcgcagcg
     3301 gcgagcgcga gattcgcatg caggctgtcg ccgtcgatgt ccatggctcg ccgggctgct
     3361 tcgcgcgcgc ggtcgacgga cgccgcatcg gcgccgtagc cgaacaggta ttgctcggtg
     3421 ccgagcagcg acaacccggc ccaagcctcg gcggagcccg gctcgcggac cgtcgcggct
     3481 tcgtagcacg cgcgcgcatc cgcgtgccgc gctgcgtcga gcacgcgccg atactggtaa
     3541 tacttgaaca ggcagtcgga caaggtcagc gcggacgccg ctaccgcacc gacgcgttgg
     3601 agctcggatt cgaagatggg gccataggct tctgcgacgg ccgcgaggcg ccgggcgagc
     3661 gaccgttgcc cgaccggatc gcgcaacgaa gcgagcggct cgtcgtacgc cgagctccat
     3721 agctgggtac ccgtcccggg cgccagtagc cgagcggtca ctctgacctg acccgccgtc
     3781 acgcgcacgc tgccactcaa gacgtaagcg ccggagtcgg cagccgcctg ccccgcttcg
     3841 ccctgcgtcg ccaccacgaa caagtgtggg tcgtcgagca acaacaaaag ctcctctcgc
     3901 agcgtcgccg ctaaccggct aatcgccggc tcgtctcctc cgagatcttc gaacggcaac
     3961 acgacgatcg gcggcttgcc gcccgcgcga gcgcgccctt ggacggcaag cgccggaccg
     4021 ccggtgaggc gcgacagctc gaagtgctgc agcaggacga cgcccaagcc ggcgagcacc
     4081 acggccgcta tcagcagcga gcgcacacgg cgccagcgcc gccggttgcg cgccgagtcg
     4141 ttggcgtccg gagtggccgg cggcatcggc cccagcaaga cttctgcggg cgcggggtgg
     4201 tacgccgacg tcaccgtata gctgccgcgc ggcaactcga ttcgcaccgc gtcgttgcgt
     4261 ccttcgtcgg cgtagtactc ggtcaggcgc cgccgcaggc ggccggcctc gactcgcacg
     4321 agcgggtccg attgggcgtc gaaatccggc ggccggccga acacctcgat cgcgatcgtg
     4381 taacccttga ggcgatcgcc ttgcccggcc agcgtttgct cgacgacgaa gcgcagaaat
     4441 ttcgatgagc gcgcagcttg ctcgaaacag cggctggcaa gaatgcgctc gagcgccgcg
     4501 cgtacttcga cggcgctggg cggcggtgcg ccaacgggat cgcgagcgac gttctgtgtc
     4561 gtttccgaaa tggggaccac cggtccggtc ggacggcggt tgccgcccga ttggaatcat
     4621 tccccggaac gacagtgtag gcctattcgc ccccggtccg aagcctcgtc gcgcccgtgt
     4681 tggggtccgc gtactcgcgg ttatcctcgc agacgtactc gataatttcg tagtccggca
     4741 tgcgcttgta cgcaaacgtc accgtccacg gcttctcgag cacggcgggg tcttcgatcg
     4801 tgatctcgtc ccagaggatt tccggcgtca cgagcttgaa acgttccgtg atgtgcatgt
     4861 cacgcgtatg cggcacgtcg cggaagcgca cgtagtcttt gataccgatc gtgtcgacga
     4921 cgagcgtgtc gccctcccac ttgccgacgg accggccgta gaacccgagc tcgatgtcgt
     4981 cgagcgtcag ctgcggcttg tcgagataga tgcggcgaat ctggctgtac gcttcctgcg
     5041 cgatgttgat ttgcgtcgca ttctggacga tctcgaacgg gaacggcccg ttcatcatcg
     5101 acggcacgcc gtccgggatg cagtgcgtga cgttggtcgc cggcggctcg ccgcgcgcgt
     5161 cggcttcacg ctgcgctgcg cggcgcttgt cccactccgc ttggaaattg gctttcaacg
     5221 gcgggggcgc gtcgggcggc gggttcgtg
//
LOCUS       DATLEE010000357         7779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6165015, whole genome shotgun sequence.
ACCESSION   DATLEE010000357 DATLEE010000000
VERSION     DATLEE010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6165015"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..497)
                     /locus_tag="VM692_07675"
     CDS             complement(75..497)
                     /locus_tag="VM692_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HUQ52086.1"
                     /translation="MPRSKSRALISEINVVPYVDVMLVLLVIFMIATPLMTQGVVVNL
                     PDVEAAAVDVARDEPLILTVDSTNRFYLSVGGDAEQPLDEQTVLERVGAVVRRNAATP
                     IYVRGDESATHGQVMHGFALLRRAGAKQAVLVVESPEP"
     gene            complement(666..974)
                     /locus_tag="VM692_07680"
     CDS             complement(666..974)
                     /locus_tag="VM692_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HUQ52087.1"
                     /translation="MLELRPTCENCNKALPPSSTDAWICSYECTFCSACVETVLGNVC
                     PNCGGGFVPRPIRPVKNWKNDNFLGKDPASTKVKHRPVDSTAHAAFAAPIKGIPPKDR
                     "
     gene            1101..1535
                     /locus_tag="VM692_07685"
     CDS             1101..1535
                     /locus_tag="VM692_07685"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52088.1"
                     /translation="MSQRNSSSWSLLVVGVTLAAPISAHHSFGAEYDVNAPITLRGVL
                     TKIEWTNPHTHIYLDVKDEKGTVVNWQLEGYPPVALHRIGWKRDVTMKVGDTVTVTGW
                     RARDGGAWGHSREITLASGEKMMFGPPAGTGDGGGSPAVDVR"
     gene            1532..2350
                     /locus_tag="VM692_07690"
     CDS             1532..2350
                     /locus_tag="VM692_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52089.1"
                     /translation="MRIVAVTLAALVACMPAAAQAPQAAPAPRTASGKPDLTGVWQGG
                     STLAGNWDDANGGVGVGGSGRTPNAPVVGSSNDRPANREGAPYQDWAAQKVLEAFNRR
                     GIDDPTGQCLPAGIPRSVMLGLFPQQFVQTPTQLVILYEYMSTFRVIPFASAHPDDLI
                     PSYMGNSIASWDGDTLVVDIIGFNDKTWLAGTGTFHSDALHIVERYTRVSKDRIDYVV
                     TMEDPNVLTKPWTLQSSLMLREGTRLQEYVCAENNLDPDRYEQLLRNGVDFTRH"
     gene            complement(2387..2914)
                     /locus_tag="VM692_07695"
     CDS             complement(2387..2914)
                     /locus_tag="VM692_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HUQ52090.1"
                     /translation="MLYDLGSYRVQTVGDEFYVAPSASVIGRVFLGRHANVWFNAVLR
                     GDVNDIRIGDDSNVQDTAVVHVDSDAACTIGNSVTIGHGATVHGCTVGDFSLVGIGAT
                     ILSHAVIGKYCLVGAGALITERKVFADRSLIIGAPARRIRDLTDEECRGLEKSAANYA
                     ERGRQYRRELSARDR"
     gene            complement(2916..3689)
                     /gene="gpmA"
                     /locus_tag="VM692_07700"
     CDS             complement(2916..3689)
                     /gene="gpmA"
                     /locus_tag="VM692_07700"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HUQ52091.1"
                     /translation="MNNKKRPAPGKLVLLRHGQSDWNQKNLFTGWHDVDLTAQGRDEA
                     RQAGRTLSAAGLTFDLAFTSVLTRAIRTLWLVLDEMKLTWIPVEKSWRLNERHYGSLQ
                     GLDKAQTTAKHGEAQVKIWRRSYDIPPPPLSESDERHPKHDPRYAGLAPTVLPGTESL
                     QTTLARVRPYWEDRIAPEILSGRNVLVAAHGNSLRAVLKMLEGVSDDAITEINIPTGV
                     PRLYELDANLRPQRAEYLGDAAAIAAAAEAVANQAKAKK"
     gene            3827..4687
                     /locus_tag="VM692_07705"
     CDS             3827..4687
                     /locus_tag="VM692_07705"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HUQ52092.1"
                     /translation="MTTKPGPDKDAATTPAGEPSGANELRYDTYLQLDTLLSAQRPIS
                     NAPEHDEMLFIIQHQVAELWFKLILHELDAAIECLKSDELPRTIKILNRVKQVQHQLI
                     NQWSVLATLTPNEYAAFRDALGSASGFQSPQYRLLEFKLGRKDRSVLSRFSHHLEYYR
                     ELKQALEQPSLYDEFLRYLYRVGHRVPEARVERDWTQMYEHHPAVVEVLQTIYQHPDQ
                     YWAAYEVSERLVDIEINFQSWRFHHMKTVERIIGGKAGTGGTSGVKFLQRALDTIFFP
                     ELIEVRTKIG"
     gene            complement(4695..5789)
                     /gene="zapE"
                     /locus_tag="VM692_07710"
     CDS             complement(4695..5789)
                     /gene="zapE"
                     /locus_tag="VM692_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020409640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HUQ52093.1"
                     /translation="MTGPLALYRQRVARGELLADGSQARVAEAFDRLYRDLQRAAPPR
                     GLRSKVAKLAGKRRKAVRGIYLWGSVGRGKTLMMDLFYNALPFRDKQRQHFHRFMAAV
                     HEGLKQHRNVENPLELVAEKIASETRIICFDEFVVTDIADAMLLGNLFAGLFARGVTL
                     AATSNIVPDQLYAGGLQRQRFLPTIALLKEHTEVVHADGGVDYRLRALERADVYQTPT
                     GEVADRRLAEFFESIAPDEGDHGGTLELLGRAIPYRRAADGAIWFEFESICDGPRGKD
                     DYIEISRLYQTVLVSNVPRFDATLENQARRFIALVDEFYDRRVKLILSAAAPATEIYR
                     GERLKHDFLRTQSRLTEMQSHDYLAQAHRA"
     gene            complement(5786..7726)
                     /locus_tag="VM692_07715"
     CDS             complement(5786..7726)
                     /locus_tag="VM692_07715"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HUQ52094.1"
                     /translation="MNTPIWQPSVERSEASNLRRFIVANRERLRGDDYAALYDWSVAQ
                     PAEFWAAVWQFCEVRASRRYDTVLRDGARMPGAKWFEGARLNFAENLLEHDATGAALI
                     FANERGERAELEWDELRAQVASVAARLRALGVGKGDRVAAFIANRPEAVVAMLATASI
                     GAVWSSCSPDFGVDAVLDRFGQIEPKVLFVTDGYFYNGKTIDSLPTVRAVAAQLQGLA
                     AVVVVPYLTKKPDVQGLPNAQLFATLLEQRAELRYEAVAFDDPLYILYSSGTTGVPKC
                     IVHGVGGTLLQHRKEHALHTDIRAGDVVFYFTTCGWMMWNWLVTALASQATLVLYDGA
                     PLHPDPGILWRLAERERISVFGTSAKYLSALEKAGFERPAQRGLPALRSILSTGSPLA
                     PSSFDYVYRSIKADVQLASIAGGTDLISCFALGNPLLPVYRGELQCRGLGMRVEIFTP
                     DGRPVREQKGELVCTAPFPSMPIGFWNDVNGRKYRAAYFERFANVWHHGDYAELTAHD
                     GIVIYGRSDAILNPGGVRIGTAEIYRIVEQFAEVAESIVVGQEWGDDTRVVLFVRLQP
                     GATLDSGLERRLREAIKTKASPRHVPAKILAVADIPRTMNGKIVELAVREAIHGRPIG
                     NRDALANPQALEHFVDRPELKS"
BASE COUNT         1354 a         2492 c         2568 g         1365 t
ORIGIN      
        1 tggcacgaac ctcggccgga aatgccgcga tgtactcgtc gacgttgctt gcggcaggac
       61 gcacggttcg agggctaggg ttccggcgac tcgacgacga gcacagcctg cttcgcaccg
      121 gcgcggcgca gcaacgcaaa cccgtgcatg acttgcccgt gcgtcgcgct ctcgtcgccg
      181 cgcacgtaga tcggcgtcgc ggcattgcgg cgcaccacgg cgccgacgcg ctcgagcacg
      241 gtttgctcgt cgagcggctg ttccgcgtcg ccgccgacgc tgaggtaaaa cctgttcgtg
      301 ctgtcgaccg tcaagatcag cggctcgtcg cgcgcgacgt cgaccgccgc ggcttcgacg
      361 tcgggcagat tcacgacgac gccctgagtc atcagcgggg ttgcgatcat gaaaattacg
      421 agcagcacga gcatgacgtc gacgtagggg acgacgttga tctcgctgat cagcgcccga
      481 gatttcgatc gcggcatgac gaagctccgc ggtgtggtgg gcgtcactag agcatcgcgc
      541 atcgagcccg tcaaatcgcg cgctcgcgcc gtgccgaagc tattcgccgc gcagcggctc
      601 cgcaccttgc atgagccaag ccgtcagcgc acgcgccggc ctacgtgcat gccgtaagtg
      661 gtgcgctacc ggtctttcgg tggaatgccc ttgatcggcg ccgcgaacgc tgcgtgcgcc
      721 gtggagtcga cggggcggtg cttgaccttc gtgctcgccg gatctttgcc gaggaagttg
      781 tcgtttttcc agttcttcac ggggcgtatc ggccttggca cgaaaccgcc gccgcaatta
      841 gggcagacgt tgccaagcac cgtctcgacg cacgccgagc aaaacgtgca ttcgtagctg
      901 caaatccacg cgtccgtgga gctcggcggc agcgctttgt tgcagttctc gcacgtcggc
      961 ctcagctcga gcatggctgt gcactctagc ccgagtgtcc ggccgcggcc aggtccacgg
     1021 cgttggcgta tattgacggc acgcgcctcc gcgtgagcac atcggcacgc gctgtggcgc
     1081 atccatcgaa ggggggagcc atgagccagc gaaactcgtc gtcttggtcc ttgctcgtcg
     1141 tcggcgtaac gctggcggcg ccgatctcgg cccaccactc gttcggcgcc gagtacgacg
     1201 tcaacgcacc gatcacgttg cgcggggtgc tgacgaagat cgagtggacg aacccgcaca
     1261 cgcacatcta tctcgacgta aaggacgaga agggcacggt cgtcaattgg cagctcgagg
     1321 gctacccgcc ggtggcgctg cacagaattg gttggaaacg cgacgtcacg atgaaggtcg
     1381 gcgacaccgt cacggtgacg ggttggcgcg cgcgcgacgg cggcgcgtgg ggccactcac
     1441 gcgagatcac gctggcgagc ggcgagaaga tgatgttcgg tccaccggca ggcacggggg
     1501 acggcggcgg cagcccggcc gtcgacgtgc gatgagaatc gtcgctgtta cgctcgcggc
     1561 gctcgtggcg tgtatgccgg cggcggcgca agcaccgcaa gcggcgccag caccgcgcac
     1621 agctagcggc aagccggacc tcacgggcgt ctggcaaggc ggcagcacgc tggccggcaa
     1681 ctgggacgac gcgaacggcg gcgtcggcgt cggcggctcg gggcgcactc cgaacgcgcc
     1741 ggtcgtcggc tcctcgaacg accggccggc caaccgcgaa ggggcgcctt atcaagattg
     1801 ggccgcgcaa aaggtcctcg aggcgttcaa tcgccgcggc atcgacgatc cgaccggcca
     1861 atgcttaccg gccggcattc cgcgcagcgt aatgctcggc ctctttccgc agcagttcgt
     1921 gcagacgccg acgcagctcg tgatcctcta cgagtacatg agcacgttcc gcgtcatccc
     1981 cttcgcctcg gctcaccccg acgacctcat tccgagctac atgggcaact cgattgcgag
     2041 ttgggatggc gacacgctgg tcgtcgacat catcggcttc aatgacaaga cctggctcgc
     2101 gggaaccggc acctttcact ccgacgcgct gcacatcgtc gagcgctata cacgcgtcag
     2161 caaagatcgc atcgactacg tagtgacgat ggaagatccg aacgtgctca cgaagccctg
     2221 gacgctgcag tcgagcctga tgctgcgcga gggcacgcgc ttgcaggaat acgtttgcgc
     2281 cgagaacaac ctcgaccccg atcggtacga gcagctgctg cgcaacggcg tcgacttcac
     2341 gcggcactga gctcgaggcg ccgagcgcgc cgagcggtgc tccggatcag cggtcgcgcg
     2401 ccgaaagctc gcggcggtac tgtcggcccc gctccgcata gttggcggca cttttctcga
     2461 gcccgcgaca ctcttcgtcc gtaaggtcgc gtatgcggcg cgccggtgcg ccgatgatca
     2521 gtgagcgatc ggcaaacact ttgcgttcgg tgatcaacgc gcctgccccg acgaggcagt
     2581 acttgccgat gacggcgtgg ctcaggatcg tcgcgccgat gccgacgagc gagaaatcgc
     2641 cgacggtgca gccgtgcacg gttgcgccgt gaccgatcgt gacgctgttg ccgatcgtgc
     2701 aggcggcatc ggagtcgacg tggacgacgg ccgtgtcctg cacgttgctg tcgtcgccga
     2761 tgcggatgtc gttgacgtcg ccgcgcagca cggcgttgaa ccagacattg gcgtggcggc
     2821 cgagaaacac ccggccgatg acggacgcgc tcggcgcgac gtagaactcg tcgccgaccg
     2881 tctgcacgcg gtacgagccg agatcgtaga gcacgctact tcttcgcctt ggcttggttg
     2941 gcgacggctt cggcggcggc cgcgattgcg gccgcatcgc cgagatattc ggcgcgctga
     3001 ggtctcaggt tcgcgtcgag ctcgtacaag cgcggcacgc ccgtcggaat gttgatctcc
     3061 gtgatcgcgt cgtcggaaac gccctcgagc atcttcaaga cggcacgcag gctgttgccg
     3121 tgcgcggcca cgagcacgtt gcgcccgctc aagatttccg gcgcgatgcg gtcctcccag
     3181 tacggacgca cgcgcgcaag cgtcgtctgc agggattccg ttccgggcag cactgtcgga
     3241 gccaggcccg cgtaacgcgg gtcatgcttc gggtgacgtt catcgctctc cgacagcggc
     3301 ggcggaggaa tgtcgtagct gcgccgccaa atcttcacct gcgcttcgcc gtgcttagcc
     3361 gtcgtctgtg ccttgtcgag gccctgtaat gagccgtagt gacgctcgtt caaacgccaa
     3421 gatttctcaa cgggaatcca cgtgagcttc atctcgtcga gcacgagcca cagtgtccgg
     3481 atggcgcgcg tcagcaccga cgtgaatgcg aggtcgaacg ttaggccggc cgcgctcagc
     3541 gtgcggcctg cctgccgcgc ctcgtcacgg ccttgagccg tcagatcgac gtcgtgccag
     3601 cccgtaaaaa gattcttctg attccagtcg ctctgcccgt ggcgcagtag cacgagcttg
     3661 cccggagcgg gccgcttctt gttgttcata taactattgt cccagatcgg cgcggagcgc
     3721 ggaaccattg ctgtgacgcg cgtcgctgcg acctcgcggg cgccgcgaaa aaactgggtt
     3781 atagtggatt cgcgcacaat ctcgtgcatg cctagtccaa ccgacaatga caacaaaacc
     3841 cggaccggac aaagatgctg caacaacacc cgcgggcgaa ccgtccggcg cgaacgagct
     3901 tcgctatgac acgtatttgc agctcgacac cttgctcagt gcgcagcggc cgatcagcaa
     3961 cgcccccgag cacgacgaga tgctgttcat catccagcat caagtcgcgg agctttggtt
     4021 caagctgatc ttgcacgagc tcgatgccgc gatcgaatgc ctgaagagcg acgagctgcc
     4081 gcgcacgatc aagattttga atcgcgtgaa gcaagtgcag caccagctca tcaatcagtg
     4141 gtcggtgcta gccacgttga cgccgaacga gtacgcggcg tttcgtgacg cgctcggctc
     4201 tgcgtcgggc ttccagtcgc cgcaatatcg cttgctcgag ttcaagctcg gtcgcaagga
     4261 tcgctccgtg ctgtcgcgct tcagccatca cctcgagtat taccgcgagc tgaagcaggc
     4321 actcgagcag ccgagccttt acgacgaatt cttgcgctat ctctatcgcg taggccatcg
     4381 cgtacccgag gcccgcgtcg agcgcgattg gacgcagatg tacgagcatc accccgcggt
     4441 cgtcgaggtc ttgcagacga tctatcagca cccggatcag tattgggctg cctacgaagt
     4501 gtcggagcgg ctcgtcgaca tcgagatcaa cttccagtcg tggcgcttcc atcacatgaa
     4561 gaccgtcgag cgcatcatcg gcggcaaagc gggcacgggc ggcacgtcgg gagtaaagtt
     4621 tctccaacgc gcgctcgaca cgatcttctt ccccgagctc atcgaggtcc gcacgaagat
     4681 cggctgacgc gccgttacgc gcggtgcgcc tgggcgaggt aatcgtggct ttgcatttcc
     4741 gtgaggcggc tctgcgtgcg caggaaatcg tgcttcagac gttcgccgcg gtaaatctcc
     4801 gtcgcgggcg ccgcggccga taggatcagc ttgacgcgcc gatcgtagaa ctcgtcgacg
     4861 agtgcgatga atcttcgggc ttggttttcg agcgtcgcgt cgaagcgcgg cacgttcgac
     4921 acgagcacgg tctgatagag ccgcgagatc tcgatgtaat cgtccttacc gcgcggccca
     4981 tcgcagatgg actcgaactc gaaccagatg gcgccgtctg ccgcgcggcg gtacgggatt
     5041 gcgcggccaa gcagctcgag cgtgccgccg tgatcgccct cgtcgggcgc gatcgactca
     5101 aaaaattccg cgaggcgccg gtcggcgact tcgcccgttg gcgtctgata gacgtcggcg
     5161 cgctcgagcg cccgcaaccg atagtcgaca ccgccgtctg cgtggacgac ctccgtgtgc
     5221 tccttcaaga gcgcgatcgt cggcaagaag cgttgccgct gcagtccgcc tgcgtagagc
     5281 tggtcaggaa cgatgttcga cgtcgcggcc agcgtcacgc cgcgcgcgaa caatccggca
     5341 aacaagttgc cgagcagcat ggcgtcggca atgtcggtga cgacgaattc gtcgaagcag
     5401 atgatgcgag tctcgctggc gatcttctct gcaacgagct cgagcgggtt ctcgacgttg
     5461 cgatgctgct tcagcccctc gtgcacggcc gccatgaagc ggtgaaagtg ctgccgttgc
     5521 ttgtcgcgga acggcagggc gttatagaag agatccatca tgagcgtctt gccgcggccg
     5581 acgctgcccc agaggtaaat cccccgcacg gctttgcgac gcttgcccgc gagtttggcg
     5641 actttgctgc gtaagccgcg cggcggcgcg gcgcgctgca agtcgcgata caggcggtcg
     5701 aacgcttctg cgactcgcgc ttgggagccg tcggcgagca gctcgccgcg cgcgacgcgc
     5761 tgacgataga gtgcgagtgg accggtcacg atttcagctc gggtcgatcg acgaagtgct
     5821 cgagcgcttg cggattggca agtgcgtcgc ggttgccgat cggccggcca tgaatcgcct
     5881 cgcgaaccgc gagctcgacg attttgccgt tcatcgtgcg cgggatgtcc gcgaccgcga
     5941 gaatcttcgc cggcacgtgc cgcgggctgg ctttggtctt gatggcttcg cggagccgcc
     6001 gctcgagacc ggagtcgagg gttgcgcctg gctgcaaccg cacgaacaag acgacgcgcg
     6061 tgtcgtcgcc ccattcttga ccgacgacga tgctctcggc gacttccgcg aactgctcga
     6121 cgatgcggta gatctcggca gtgccgatgc gcacgccgcc ggggttcagg atcgcgtcgg
     6181 agcggccata gatgacgatg ccgtcgtgcg ccgtgagctc cgcgtaatcg ccgtgatgcc
     6241 agacgttcgc gaagcgctcg aaatacgccg cgcgatactt gcggccgttc acgtcgttcc
     6301 agaagccgat cggcatcgat ggaaacggcg ccgtgcagac gagctcgcct ttctgctcgc
     6361 gcacggggcg gccatcgggc gtgaagatct cgacgcgcat gccgaggccg cggcattgca
     6421 gctcgccgcg gtacacgggc aacagcggat tgcccaaagc gaagcacgag atcaggtctg
     6481 tgccgcccgc gatcgaggcg agctgcacgt cggctttgat gctgcggtac acgtaatcga
     6541 agctcgacgg cgcgagcggc gagcccgtcg acaagatcga gcgcaacgcc gggaggccgc
     6601 gttgcgcggg ccgctcgaac ccggccttct cgagcgcgct cagatatttc gcgctcgtgc
     6661 cgaagacgct gatgcgctcg cgttctgcga ggcgccagag gatgccgggg tcggggtgga
     6721 gtggggcgcc gtcgtacagc acgagcgtcg cttgcgaagc cagcgccgtg acgagccagt
     6781 tccacatcat ccagccgcaa gtcgtgaaat agaacacgac gtcgccggcc cgaatgtcgg
     6841 tgtgcagcgc gtgctctttg cgatgctgca gcagcgtgcc gccaacgccg tgcacgatgc
     6901 acttcggcac tccggtcgtg cccgatgaat agaggatgta gagcgggtcg tcgaaggcga
     6961 cggcctcgta gcgcaactcg gctcgttgct cgagcagcgt tgcgaacagt tgggcgttcg
     7021 gcagcccctg cacgtcaggt ttcttggtca ggtagggaac gacgacgacg gccgcgagtc
     7081 cttgcagctg cgcggcgacg gcgcgcaccg tcggcagcga gtcgatggtc ttgccgttgt
     7141 agaagtagcc gtcagtcacg aacaagacct tcggctcgat ttgcccgaag cggtcgagca
     7201 cggcgtcgac gccgaagtcc ggcgagcacg acgaccagac ggcgccgatg ctcgcagtgg
     7261 ccagcatcgc gacgacggct tcgggacggt ttgcgatgaa ggcggcgacg cggtcgcctt
     7321 tgccgacacc gagcgcgcgc aggcgggccg cgacgctcgc gacttgtgcg cgcaactcat
     7381 cccactcgag ctcggcacgc tcgccgcgct cgttggcgaa tatcagggcg gcgcccgtcg
     7441 cgtcgtgctc gagcaggttc tcggcgaagt tcagccgcgc gccttcgaac cacttcgcgc
     7501 cgggcattcg cgcgccgtcg cgcagcacgg tgtcatagcg tcggcttgca cgaacctcgc
     7561 agaactgcca aacggccgcc caaaactccg caggctgcgc gacgctccag tcgtagagcg
     7621 cggcgtaatc gtcgccgcgc aatcgctcgc ggttggccac gatgaagcgg cgcaggttgc
     7681 tcgcttcgct gcgttcgacg gacggctgcc agatcggggt gttcaagcgg tcgcgacaac
     7741 ggggacgtgt gagacgcgat gttacaatgc gcagcgttc
//
LOCUS       DATLEE010000358         3997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1152729, whole genome shotgun sequence.
ACCESSION   DATLEE010000358 DATLEE010000000
VERSION     DATLEE010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152729"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..583)
                     /locus_tag="VM692_07720"
     CDS             complement(53..583)
                     /locus_tag="VM692_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HUQ52095.1"
                     /translation="MQLSSAHERSRGRHAGFTLIELMIVVAIIGILASMAIPAFQNYS
                     IRAQVSEGLNLAAASKAPIALAFLNDGAAPANRAEAGLSPTASDSSGRYVTSIDVTNG
                     VLVVTYGYVASALIAGLTLTLTPYETADNSVVWRCGMAAAPAGLSGIGTSGGGNAAAY
                     IAPTVPVQYLPASCRL"
     gene            complement(618..896)
                     /locus_tag="VM692_07725"
     CDS             complement(618..896)
                     /locus_tag="VM692_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52096.1"
                     /translation="MEVRVLTAPPSSGYPEQDYICVHTDTDPLDDTVVLATIERVTDR
                     TKGKVKRVKTLLARQPMSADAALDLATSYAEWKQVPVVYADPLARETR"
     gene            complement(1145..1483)
                     /locus_tag="VM692_07730"
     CDS             complement(1145..1483)
                     /locus_tag="VM692_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HUQ52097.1"
                     /translation="MGRKLYVGNLPYSASEQSVRDAFSKCGTVSSVSIISDRDTGQSK
                     GFGFVEMSSDAEAQAAIQQLNGTSLDGRQIKVNEAKPKAPGGGGGGGGGGGRGGYGGG
                     GGGGGGRDRW"
     gene            complement(1771..2826)
                     /locus_tag="VM692_07735"
     CDS             complement(1771..2826)
                     /locus_tag="VM692_07735"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HUQ52098.1"
                     /translation="MNAALTLAAEHDLAGRYDDAVNALARATQNGDLDAMTELGKRLV
                     VGEKAPLLPKDGAGLLHEAAKGGNAEAACRLATLTALGAHVTQSWSDAFGLLLFAAER
                     GSESARGQLQALAGRAPDAGHGEQRWRRLAESTDLRYWLSTPQGQTLCADPLVRVFPQ
                     FATASVCAWLIEQARPSLKRALIYDPVGGKDIADHMRTNSAAGFDLIHADVVQAAVQW
                     RMSIAVGVPIEHMEGPTVLHYAIGEQITNHYDFVNPRIQNYQAEIEKRGQRIVTFLVY
                     LNDDYEGGETDFPNLGLRYHGAKGNGIFFTNALPDGQPDLRMVHAGLPPKDNEKWLMS
                     QFIRNRVVLGARAENQA"
     gene            2964..>3997
                     /locus_tag="VM692_07740"
     CDS             2964..>3997
                     /locus_tag="VM692_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HUQ52099.1"
                     /translation="MNATSPEAPSPSFAELFTPKLVTVIREGYGWQQLRADALAGLTV
                     AIVALPLSMAIAIASGASPAQGLYAAIFGGFLVSALGGSRFQIGGPAGAFIVLVATTV
                     QAHGLEGLLLATMLAGLMLAAIGLLKLGTYIKFIPYPVTVGFTAGIAVIILSSQVKDL
                     LGLTLAEPEPGELLDKLPVLWRYLPTLDGTTIAVSLATIVVIATLRRWRPQWPGMLLA
                     VAGAAVVTAGAHLDVMTIGSRFGGIPSGLPLPSLPEMSVDKVREVLPSAVAFALLGAI
                     ESLLSAVVADGMTGRRHRSNCELVAQGAANVVSSLFGGICVTGTIARTATNVRAGAHG
                     PVAGMLHSVF"
BASE COUNT          635 a         1309 c         1307 g          746 t
ORIGIN      
        1 accctcgtcg tttacgaggc ctggctcgcc ggcacgttcc tgtagggggt cgtcagagcc
       61 ggcagctcgc gggcaagtat tgcaccggca ccgtcggtgc gatgtaggcc gcggcattgc
      121 cgccgcccga cgttccgatc ccgctcaagc cggcgggcgc cgcggccatt ccacagcgcc
      181 ataccacgct gttgtcggcc gtctcgtagg gcgtcagcgt caacgttagc cccgcgatca
      241 gcgcgctcgc cacgtacccg taggtgacga cgagcacgcc gttggtaacg tcgattgacg
      301 tgacgtagcg ccccgacgag tccgatgcgg tcggcgagag acccgcttcg gcacgattcg
      361 cgggcgccgc cccgtcgttc agaaacgcaa gcgcgattgg ggccttcgaa gccgccgcca
      421 agttcaagcc ttcggacact tgcgcgcgga tcgagtagtt ctgaaacgcc gggatcgcca
      481 tcgacgccag gatgccgatg atggcaacga cgatcatgag ctcgatcagc gtgaagcccg
      541 catgccggcc tcgcgagcgc tcatgcgcag agctaagttg catctgaaac tccaactttt
      601 cagcaattcg gttggctcta gcgcgtctcg cgcgcgagcg ggtcggcata gacgacgggg
      661 acctgcttcc actcggcata gctcgtagcg aggtcgagcg cagcgtcggc cgacatgggt
      721 tggcgagcga gcagcgtctt cacgcgcttc accttgccct tcgtgcggtc cgtgacgcgc
      781 tcgatcgtcg cgagcacgac ggtgtcgtcg aggggatccg tgtcggtgtg cacgcaaatg
      841 tagtcttgct ccgggtaacc cgaagaaggc ggtgccgtta gcacgcggac ttccatctcg
      901 tttctccgcc ccaaaaactc agatcactga gcagggcaga gcaagacgca tgccactaac
      961 ggcgctgcgc catttgcgta ggtcgtgcgc gagttatgtc gagtacgagt gacgcgctgc
     1021 gtcagttacg gtcgtaatgg ttcttgacac aattgggccg acgagaaacg cggcacgaaa
     1081 aaaaacgccg cattgctgcg gcgtttctta gttctctcgt gaaagagatc gaatgcgtta
     1141 gcgcttacca gcgatcgcgt ccgccgccac cgccgccacc gccgccgtag ccgccgcgac
     1201 caccgccgcc gccgccacca ccaccaccac ccggggcctt cggcttggcc tcgttgactt
     1261 tgatctgccg gccgtcgagt gacgtgccgt tcagttgttg gatcgcagcc tgtgcttcgg
     1321 catcgctcga catttcgacg aagccgaaac ctttgctctg accggtgtca cgatcgctga
     1381 tgatggaaac cgaagacacg gtgccgcact tgctgaacgc atcgcggaca ctttgttcgg
     1441 aagccgaata cggcaagttg ccgacgtaga gttttctgcc catggtacct ccccaggtag
     1501 cgcgctcgct caagagcaca aggcaccatg ccaagcagac tcatcgaagc gatcgacgga
     1561 cggcgggata atacgtatta acgtgctagg acgccagtgg gcaggccatt ttcaagtgtt
     1621 ttcgcggggt tagcctgccg gttcgatgcg taaaatagcc ccgtcgtcgt catctaccga
     1681 gcacgtagag cggcccgtcc gggccccggc ggatgtccgc ttgcgatggc gccagtccgt
     1741 cagcagcgct tcgccggtgc gcggtccggc tcaggcctgg ttctccgcgc gcgcgccgag
     1801 cacgacgcga ttgcggatga actgcgacat cagccacttc tcgttgtctt tcggcggtaa
     1861 gccggcgtgc accatgcgca agtcgggttg gccgtccggg agagcattcg tgaagaagat
     1921 tccgttgccc ttggcgccgt gataacgcag cccgagattc ggaaagtccg tttcgccgcc
     1981 ttcgtagtcg tcgttcaaat agacgaggaa ggtgacgatg cgctggccgc gcttctcgat
     2041 ctcggcttga tagttctgga tgcgggggtt cacgaagtcg tagtgattcg tgatctgctc
     2101 gccgatcgcg tagtgcagca ccgtcgggcc ttccatgtgc tcaatcggca cgcccaccgc
     2161 gatactcatg cgccactgca cggccgcttg gacgacgtcc gcgtggatga gatcgaagcc
     2221 ggcggcactg ttcgtgcgca tgtggtcggc gatgtccttg ccgccgacgg ggtcgtagat
     2281 caacgcgcgc ttcaagctcg ggcgcgcttg ctcgatgagc cacgcgcaga cggaggcggt
     2341 cgcgaattgc ggaaacacgc gcacgagcgg atcggcacac agcgtttggc cttgcggcgt
     2401 gctgagccaa tagcgcaggt cggtgctctc ggcgagacgc cgccaccgct gctcgccgtg
     2461 gccggcatcg ggcgctcggc ccgccagcgc ctgcaattgg ccgcgcgccg attccgagcc
     2521 gcgctccgct gcgaacaaga gcaggccgaa cgcatcgctc cagctttgcg ttacgtgcgc
     2581 gccgagcgcc gtcagggttg cgagccggca cgccgcttcc gcattgccgc ctttcgcggc
     2641 ctcgtgcagt agccccgcac cgtctttcgg caacagcggc gctttttcgc cgacgacgag
     2701 ccgcttgccg agctcggtca tggcgtccaa gtcgccgttt tgcgtcgcgc gcgccaaagc
     2761 gttaacggcg tcgtcgtaac ggcccgccag atcgtgctcg gcggccaagg tgagggcggc
     2821 attcatagca aaaagcctcg cacaaacggt cggcgaccga caagccgcgg cggcccggca
     2881 gcgtaattcg gggcctgcga tgtcggtatt gtgaccttta cctatggctc ggcgcccgcg
     2941 ttgcgccgat catggcgcgg cccatgaacg ccacgtcccc cgaggcgccg agcccgagct
     3001 ttgcggagct gttcactccg aagctcgtca ccgtcattcg cgaaggctac ggctggcagc
     3061 agctgcgcgc cgatgcgctg gccggcctga cggtggcgat cgtggcgttg ccgctgtcga
     3121 tggcgattgc gatcgcctcg ggcgcgtcgc cggcgcaagg tctgtacgcg gcgatcttcg
     3181 gcggcttcct cgtctcggcg ctcggcggca gccgcttcca aatcggcggg cccgccggtg
     3241 cgttcatcgt cctcgtggcg acgaccgtgc aggcccacgg gctcgaaggc ctgttgctcg
     3301 cgacgatgct ggccggcctc atgctcgcgg caatcggctt gctgaagctc ggcacctaca
     3361 tcaagttcat tccgtatccc gtgaccgtcg ggttcacggc cggcatcgcc gtgatcattc
     3421 tgtcgagcca ggtcaaggac ctgctcggcc tgacgctcgc ggagcctgag cccggcgaat
     3481 tgctcgacaa gctccccgtg ctgtggcggt atctgcccac gctcgacggc acgacgatcg
     3541 ccgtgtcgct ggcgacgatc gtcgtgatcg cgacgttgcg acggtggcgg ccgcagtggc
     3601 ccggcatgtt gctcgccgtc gcgggcgccg ccgtcgtcac ggcgggcgcg catctcgacg
     3661 tcatgacgat cggctcgcgc ttcggcggca ttccgagcgg cttgccgctg ccgtctttgc
     3721 ccgagatgag cgtggacaag gtgcgcgagg tgttgccaag cgccgtggcg ttcgcgctgc
     3781 tcggtgcgat cgagtcgctg ttatcggcgg tcgtcgcgga cggcatgacg ggccggcggc
     3841 atcggtcgaa ttgcgagctc gtcgcgcaag gcgccgccaa cgtcgtgtcg agcttgttcg
     3901 gcggcatctg cgtcacgggc acgatcgcac gcacggcgac gaacgtgcgc gccggcgcgc
     3961 acggccccgt cgccggcatg ctgcactcgg tgttctt
//
LOCUS       DATLEE010000359         4893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1153433, whole genome shotgun sequence.
ACCESSION   DATLEE010000359 DATLEE010000000
VERSION     DATLEE010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153433"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VM692_07745"
     CDS             complement(<1..592)
                     /locus_tag="VM692_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HUQ52100.1"
                     /translation="MGKVIGIDLGTTNSCVAVMDGGKPKVIENSEGDRTTPSIVAFTK
                     DGEVIVGQTAKRQAVTNPQNTLYAIKRLIGRKFEDDVVKKDVGMVPYKIAKADNGDAW
                     VDVNGKKMAPPEISARVLTKMKQTAEDYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKAGGDRKIAVYDLGG"
     gene            complement(665..1159)
                     /locus_tag="VM692_07750"
     CDS             complement(665..1159)
                     /locus_tag="VM692_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HUQ52101.1"
                     /translation="MNEDQTESAAREPDTHPDVEALRREAEENRERHLRAVAELDNLR
                     KRSAREVDAARKFGAERLAQAVLPVHDSLEAGLAAAEKAGETALLEGQRATLRLLDEA
                     FNASGIREINPVGELFDPNKHEALSLLAAPNAAPNTVIQVVQKGYELNERLLRAAKVI
                     VARE"
     gene            complement(1226..2281)
                     /gene="hrcA"
                     /locus_tag="VM692_07755"
     CDS             complement(1226..2281)
                     /gene="hrcA"
                     /locus_tag="VM692_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HUQ52102.1"
                     /translation="MASDKPGDVLDERAQTLLRLLVDKYIRDGQPVGSRTLSRESGLD
                     LSPATIRNIVSDLEELGFVSSPHTSAGRVPTIKGYRFFVDTLIKLTPPRGDELKRFSD
                     SLSDTSGDPQAVALSVSNLLSAVTSLAGIVTVPRRAQTALRQIEFLPLSGHRVLAILV
                     INDREVQNKILNVSRDFGPDELRRASNYLNECFGGRSLPEVRDLLLSELKRTRASMNA
                     LMVEAIEMAQKVFPESEPVPEFVVAGETNLMGLHQVADVETLKSLFEAFGRQRDILHL
                     LDQSMRADGVQIFIGHESGYELLDSYSIVAAPYATEDDTVGVLGVIGPTRMAYERVIP
                     IVDITAKLLGRALKSNS"
     gene            2367..3263
                     /locus_tag="VM692_07760"
     CDS             2367..3263
                     /locus_tag="VM692_07760"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HUQ52103.1"
                     /translation="MQREFKTVALWGRLGEHSVTEPAQQILEHLRKRGITVLASATSD
                     TSRALAGATHVDERELATRSDLVIAIGGDGTLLHAARNVAARKVPLVGINRGRLGFLT
                     DVSPEHMLDALDAILAGDYLDERRLMLAASLGQPAGGSLFALNDIVLQRGDTGRLLDF
                     TTQVDDTYVNTHRGDGLIVATPTGSTAYALSCGGPIIQPNVDALVMVPICPHTLSDRP
                     LVLPSSSTIRVTLDNAGGSEAHVVCDGEPLARMAPTDVLTISLAKQAVTLLHPRDYNY
                     YELLRSKLNWGRASRDRSKPQR"
     gene            3271..>4893
                     /gene="recN"
                     /locus_tag="VM692_07765"
     CDS             3271..>4893
                     /gene="recN"
                     /locus_tag="VM692_07765"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HUQ52104.1"
                     /translation="MLSLIRVKNYAVIDEIEVEFGGGLNVMTGETGAGKSILVDALGL
                     ALGDRADASAVRHDADRAEISVAFDVPAGHAALAWLSDRGLDDETACTLRRLLGADGR
                     SRAFINGQPVNLQDLKELGGLLVDIHGQHAHQSLLEAGSQRALLDGADDIAPLATTVA
                     EQFAMWQSLLEQLENRRSSSAQRQNEIELLRFQVAELDALALVDGEPEKLRAEHDRLA
                     NTDRLMSGVSAALDALAESEAASAYSAVILARRELEKLADVDATLRSPIAALASVEIE
                     LRETEVTLQHYRDRIEADPARLGWLDDRLARMRTLARRHNVVEEALPGILAALRERLS
                     ALDGGGESLATLEKKTGEARSRFLTTARDLSAKRAEHAAALGRSVTSLLVELGMPHGE
                     FRVELQAKPVERADATGLERVEFQVKLNPGLPFAPLAKVASGGELSRISLAIEVVRSG
                     ASPVTAFVFDEVDAGISGRVADIVGRKLKQLSSACQVLCVTHLPQVASHGEAHYRVLK
                     QTDGKSSRTEVRALSSKERVEELSRMLGGVEVT"
BASE COUNT          833 a         1624 c         1567 g          869 t
ORIGIN      
        1 cgccgcccag gtcgtatacg gcgatcttgc ggtccccgcc tgccttgtcg agcccgtagg
       61 ccaatgcagc cgcggtcggc tcgttgatga tgcgtttgac ctcgaggccc gcgatgcggc
      121 cggcatcttt ggtcgcctga cgctgcgagt cgttgaagta agccggcacg gtgatcacgg
      181 cctcggtgac cttctcgccg agatagtctt cggccgtctg cttcatcttc gtcagcacgc
      241 gcgccgaaat ttcgggcggc gccatcttct tgccgttgac gtcgacccac gcgtcgccgt
      301 tgtcggcctt cgcgatcttg tacggcacca tgccgacgtc cttcttgacg acgtcgtcct
      361 cgaacttgcg gccgatgagc cgcttgatcg cgtacagcgt gttctgcgga ttcgtgacgg
      421 cttggcgctt cgcggtttgc ccgacgatga cctcgccgtc cttcgtgaac gcgacgatcg
      481 acggcgtcgt gcgatcgccc tcgctgttct cgatgacttt cggcttgccg ccgtccatga
      541 ccgcgacgca cgaattggtc gtgcctaagt cgatgccgat cactttaccc atgtgcttgc
      601 tccagaaaac attaaaagga gaacgttagt ccgcgagatg gggcacggat acgccaattc
      661 aaccctactc gcgcgcgacg atgaccttcg cggcgcgcag caaccgctcg ttgagctcgt
      721 aacccttctg cacgacctgg atgaccgtgt tcggcgccgc attgggcgcg gccaataggc
      781 tgagcgcctc gtgcttgttc ggatcgaaca gctcgccgac cgggttgatc tcgcgaatgc
      841 cggaagcgtt gaacgcctcg tccaacagac gcagcgtcgc gcgctgccct tcgagcaacg
      901 ccgtttcgcc ggccttctcg gccgccgcca ggcccgcctc gagactgtcg tggaccggca
      961 agacggcctg agccaagcgc tcggccccga acttgcgtgc cgcgtcgact tctcgggcgc
     1021 tgcgcttgcg cagattgtcg agctcggcca cggcccgcag gtggcgctcg cgattctcct
     1081 cggcctcgcg ccgcagcgct tcgacgtcgg gatgcgtatc gggctctcga gctgcgcttt
     1141 ccgtttggtc ttcgttcatg acgatccttg agtgcactct ccccaaacgg agagtgcttt
     1201 ctcgaagctc ttcgcagatg gggggctaac tgttggactt caaggcacgg ccgagcaatt
     1261 tggccgtgat gtcgacgatc ggaatgacgc gctcatacgc catacgggtc gggccgatga
     1321 caccgagcac accgaccgta tcgtcctcgg tcgcatacgg ggctgcgacg atactataag
     1381 aatcaaggag ttcatatccg gactcgtggc cgataaagat ctgcacgccg tcggctcgca
     1441 tgctctggtc gagcagatga aggatgtcgc gctggcgacc gaacgcctcg aacaggctct
     1501 tcaaagtctc gacgtccgcc acttgatgca gacccatgag attcgtctcg ccggccacga
     1561 cgaactcggg gacaggctcg gactccggaa agaccttctg cgccatctcg atggcctcga
     1621 ccatcaaggc gttcatcgag gcccgcgtcc gcttgagctc cgacaagagc aaatcgcgta
     1681 cctcgggcag gctgcgacca ccgaagcact cattcaaata gttgctggcg cgccgtagct
     1741 cgtcggggcc gaaatctcgt gaaacgttca ggattttgtt ctggacctcg cgatcgttga
     1801 tcacgaggat ggcgagcacg cgatgaccgg acagcggcag gaactcgatc tgccgcaatg
     1861 ccgtttgtgc gcggcgcggc acggtcacga tgccggcgag gctcgtgacg gccgacagca
     1921 ggttcgatac ggatagcgcg accgcctgcg ggtcgccgga ggtgtcgctc aagctgtcgc
     1981 tgaagcgctt gagctcgtca ccgcgtggcg gtgtgagctt gatcagcgtg tcgacgaaga
     2041 atcgatagcc tttgatcgtg ggcacgcggc ccgccgacgt gtgcggcgag cttacgaaac
     2101 caagctcctc gagatccgaa acgatgttgc gaatcgtcgc cgggctcaag tccaaacctg
     2161 actcgcgcga caacgtgcgc gagcccacgg gttgtccgtc gcgaatatat ttgtcgacga
     2221 gaagacgcaa cagagtctgt gcgcgctcgt ccaaaacgtc tccgggtttg tcgctcgcca
     2281 taggcccagg ctgtgataac gtagagcccg ttttcagcga ccagcacgct agcaacgggt
     2341 tcccttcact gtcaagaaat cgagctatgc agcgtgaatt caagacagtc gcgttgtggg
     2401 ggcgtcttgg cgagcacagc gtcacggaac ccgcgcagca aattttggag cacctgcgca
     2461 aacgcggcat cacggtccta gcctctgcta cttcagacac ctcgcgcgcg ctcgccggcg
     2521 ccacgcacgt cgacgaacga gagctcgcga cgcgcagcga tctcgtcatc gcgatcggcg
     2581 gcgacggcac gctgctgcac gcggcgcgca atgtcgcggc acgcaaggtg ccgctcgtcg
     2641 gcatcaaccg cgggcggctc ggcttcctga cggacgtctc acccgagcac atgctcgatg
     2701 cgctcgatgc gattctcgcg ggcgactacc tcgacgagcg gcggctcatg ctggcggcga
     2761 gcttggggca gcccgccggc gggtcgttgt tcgcgctcaa cgacatcgtg ctgcaacgcg
     2821 gcgacacggg ccggctgctc gacttcacga cccaggtcga cgatacctac gtcaacacgc
     2881 atcgcggcga cggcctgatc gtcgccacgc cgacggggtc cacggcttat gcgctgtcgt
     2941 gcggcgggcc gatcatccag ccgaacgtcg atgcgctcgt catggtgccg atctgcccac
     3001 acacgttgag cgaccggccg ctcgtgctgc cgagctcgag cacgattcgc gtgactctcg
     3061 acaacgccgg cggcagcgaa gcgcacgtcg tctgcgacgg cgagccgctc gcacgcatgg
     3121 ccccgaccga cgtgctcacg atctcgctcg cgaagcaagc cgtgacgctg ctgcatccgc
     3181 gcgattacaa ctactacgag ctgctgcgct cgaaactgaa ctggggacgc gcgagccgag
     3241 atcggtcgaa gccccaacgc tgattgcgtc atgctgagcc tgattcgcgt caagaactac
     3301 gccgtcatcg acgagatcga agtcgagttc ggcggcggtc tcaatgtgat gaccggcgag
     3361 acgggcgccg gtaagtcgat cctcgtcgac gcgcttgggc ttgcgctcgg cgaccgcgcc
     3421 gatgcttcgg cggtgcgcca tgacgcggac cgtgccgaga tcagcgtcgc gttcgacgtg
     3481 ccagctgggc acgccgcgct ggcgtggctc tcggaccgtg ggctcgatga tgagacggcc
     3541 tgcacgttgc gtcgtctgct cggcgccgat ggccgctcgc gcgctttcat caacggccag
     3601 cccgtcaact tgcaggactt gaaggagctc ggcgggctgc tcgttgacat tcacggccag
     3661 cacgctcatc aatcgctgct cgaggccggc agccaacgcg cgctgctcga cggcgccgac
     3721 gacatcgcgc cgctcgctac gacggtcgcg gagcagttcg caatgtggca gtcgctgctc
     3781 gagcagctcg agaatcggcg ctcctcgagc gcgcagcggc aaaacgagat cgagctgctg
     3841 cgcttccaag tcgccgagct cgacgcgctc gcgctcgtcg acggcgagcc cgagaaacta
     3901 cgcgccgagc acgatcggct cgcgaacacc gaccgcctga tgagcggcgt cagcgccgcc
     3961 ctcgatgcat tggccgaaag cgaagcggcc tccgcctact ccgccgtgat actcgcgcgc
     4021 cgcgagctcg agaagctcgc cgacgtcgac gcgaccttgc gctcgcccat cgctgcgttg
     4081 gcgagcgtcg agatcgagct gcgcgagacc gaagtgacgt tgcagcacta ccgcgaccgc
     4141 atcgaagccg atccggcgcg gctcggctgg ctcgacgacc ggctcgctcg gatgcgcact
     4201 ctcgcgcgcc gccacaacgt cgtcgaggaa gctttgccag gaatcttggc ggcgctgcgc
     4261 gaacggctca gcgcgctcga cggcggcggc gagtcgctcg cgaccttgga gaaaaaaacc
     4321 ggcgaagcgc gttcgcgttt cctcacgacg gcacgcgact tgagcgccaa gcgcgccgag
     4381 cacgcggccg cgctcggccg ctccgtcacg agcctgttgg tagagctcgg catgccgcac
     4441 ggcgagttcc gggtcgagct gcaggccaag cccgtcgagc gcgccgatgc gacggggctc
     4501 gagcgcgtcg agttccaggt caagctgaac cccgggctac cgttcgcgcc gctcgcgaag
     4561 gttgcgtcgg gaggcgagct gtcgcgcatc agcctcgcga tcgaggtcgt gcgctcgggg
     4621 gcctccccgg tcacggcgtt cgtcttcgac gaggtcgacg ccggcatcag cggccgagtt
     4681 gccgacatcg tcggccgcaa gctcaagcaa ctgtcgtcgg cgtgccaagt cctgtgcgtc
     4741 acgcacttgc cgcaagtcgc gagccacggc gaggcgcact atcgcgtgct gaagcagacc
     4801 gacggcaaat cgagccgcac cgaagtgcgc gccctgtcga gcaaggaacg cgtcgaggag
     4861 ttgtcgcgga tgctcggcgg cgtcgaggtc acg
//
LOCUS       DATLEE010000360         5887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3196990, whole genome shotgun sequence.
ACCESSION   DATLEE010000360 DATLEE010000000
VERSION     DATLEE010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3196990"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /gene="dcd"
                     /locus_tag="VM692_07770"
     CDS             complement(<1..439)
                     /gene="dcd"
                     /locus_tag="VM692_07770"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HUQ52105.1"
                     /translation="MSVKSDRWIRRMADSHKMIEPFEAKQVRHVDGQRVISFGTSSYG
                     YDVRCAPEFKVFTNINSAIVDPKKFVESSFVDMAGDVCIIPPNSFALARTVEYFRIPR
                     NVLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTTLPA"
     gene            complement(439..1533)
                     /gene="apbC"
                     /locus_tag="VM692_07775"
     CDS             complement(439..1533)
                     /gene="apbC"
                     /locus_tag="VM692_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HUQ52106.1"
                     /translation="MTEALPSNLQRALGDFILPQTGRPLGGPGASFELDRRGAGWHVT
                     IRLGFPLAASSAGLVEALRVHCAPALEGAAIEFSIESAIVSHAVQHGLKPLPGVHNLI
                     AVASGKGGVGKSTVAVNFALALAAEGARVGLLDADIYGPSQPRMLGLTGRRPETRDGK
                     TLEPLVAHGLEAMSIGFLVDEQQPMAWRGPMVTSALNQLLTQTHWGDLDYLVVDMPPG
                     TGDIQLTLAQRVPVSGAVIVTTPQDIALADARKGLEMFQKVHVPVLGVVENMSLHICS
                     RCGHEEHIFGEHGGRNLSEQYGVPLLGTLPLDRRIRQETDEGKPTVAADPKGPLARAF
                     TEAALRAVGELAARAKDYSRLFPNITVEDN"
     gene            1776..4274
                     /locus_tag="VM692_07780"
     CDS             1776..4274
                     /locus_tag="VM692_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUQ52107.1"
                     /translation="MNFITALQADRLIAQIREEADPSSADGKKLFAKLGKLGTGAIPK
                     ILEALASADKRQTVEYVELLNALISDKTLPLITRGLADGDPRTVSGTAWALSSNKRFN
                     VNRLVDLLGEDDYSKAAIVEVLQAHKDRLNVRQLLGQIYFLQPSEKGAVFKLIDEVTT
                     EDMVPDLLARMDGKDPLVKTHLINVLARFDSPEVNKALQEALRDSNKLVRGAALAGIA
                     RSKTTLDVALVAGLLLDPDLDVMNKAVDVIIQLNHPETAKYLIDALKAENEFSRRSAV
                     EILNAIGTTHSVKWLLEAVADEDWWVRSRASDALARIGGPRVVDAVLDLIKDKDENIR
                     RAAIEILNTCRDKRAVDRLIDATKDKDWWVSERAADALAEIGDAKALPALLEMMAKNN
                     RSLPVAIAAIGKLNNPKVLDKILPYLQRPEKEVRVAAIGAVVQLAGEQQAEAVRPLLQ
                     QSAQGADETVVRAVTRALQKLEGKLSPSGNYRNNTTVVGATVASSTAPPAPAATVTRA
                     ATTRAAPTPPPPAPSGTATARTLLVEGPDDAVPEPPPPEAPSFDLSALQPGDMIEGRY
                     KFVQKIGKGAFGTVVLVEDTVVEERLILKFLNANVASDEEMLKRFVHELRYSRRITHK
                     NVIRIYDFLYVGGSYAISMEYFPSHTLGAEIANEKPMPPKKAVGYGTDICVGMAVAHQ
                     QGIIHRDLKPANILINETGLLKIVDFGVAAAAKSGDTQLTKTGYVIGSPKYMAPEQIL
                     GKKVDERADIYSLGVIMYEMLAGNPPYSRGDHMSVMYQHVQGKAQPLNEANPKVPREI
                     ADVVTKTMQVDKLKRYASMEELRVALEAATAAVR"
     gene            4279..5412
                     /locus_tag="VM692_07785"
     CDS             4279..5412
                     /locus_tag="VM692_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HUQ52108.1"
                     /translation="MARIDAFLKLGLAQGCSDIHLAVGIPPMLRLHGDLMPIKFRDLG
                     DTELEGYVDEILSDSQRLRFRQKNDIDFSYVSTDGGRFRVNVFHKATGVGATFRNIPA
                     VVPSLDSLGLPPILHKFCDLHQGMVLVTGSTGTGKSTTLAAMIDHLNTTSNSNIISLE
                     DPIEFVHSSKRSQIIQRELGTHIPSFAEGVRASLREDPDVILVGELRDGETISMAMTA
                     AETGHLVLGTLHTTSAVKSIDRIIDALPGDQREQTKSFLSNSLHAVVTQNLIKTPDGR
                     GRRAVVEVMVMNRAIAKLIQTEQTHQIPAQLQTGKDAGMQLMDQALLNAISAKLIDPD
                     DAYRYATDRKKFERFVTDTTVLPSGVTTTTPAGQAAGKSAGTG"
     gene            5426..>5887
                     /locus_tag="VM692_07790"
     CDS             5426..>5887
                     /locus_tag="VM692_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HUQ52109.1"
                     /translation="MSLIEDFLRSMYLRKGSDLHVIAGDPPRMRVHGDLITLDNQRLD
                     PEELRSELFGIMSEQSLEQFNKHDSADFAHYIPDVARFRVNVFRHLNGIGAVFRGIPS
                     KCLTLEELKMPKALHELATATRGLILVTGKTGSGKSTTLAGVVDAINSKIKG"
BASE COUNT         1165 a         1929 c         1859 g          934 t
ORIGIN      
        1 tcgccggcaa cgtcgtcgtg ttcgagaact cgagcgtcac gtgcccttcc cactcgggct
       61 cgagcggcgt tacgttgacg ataattccgc acctggcata cgtgcttttt ccgagacaga
      121 tggtcagcac gttgcgcggg atgcggaagt actcgaccgt gcgcgccagc gcaaacgagt
      181 tcggcggaat gatgcagacg tcgccggcca tgtcgacgaa gctcgactcg acgaattttt
      241 tcgggtcgac gatcgcggag ttgatgttcg tgaagacctt gaactcgggc gcgcaacgca
      301 cgtcgtaacc gtagctcgaa gtgccgaacg agatgaccct ttgcccatcg acgtgccgca
      361 cttgcttggc ctcgaacggc tcgatcatct tgtgcgaatc cgccatgcgg cggatccagc
      421 gatcggactt cacgctcatc aattgtcctc gacggtgatg ttcggaaaca ggcggctgta
      481 gtctttcgcg cgcgcggcga gctcgccgac ggcacgcagc gccgcttcgg tgaacgcgcg
      541 cgcgagcggc cctttcgggt cggccgcgac cgtcggcttg ccctcgtcgg tttcttgccg
      601 aatgcgccga tcgagcggca gcgtgccgag cagcggcacc ccgtactgct cgctcaagtt
      661 gcggccgccg tgctcgccga aaatgtgctc ttcgtgaccg caacggctgc agatgtgcag
      721 gctcatgttc tcgacgacgc cgagcacggg cacgtggacc ttctggaaca tctcgaggcc
      781 tttgcgggca tccgcgagcg cgatgtcctg cggagtcgtg acgatgacgg cgccgctgac
      841 gggcacgcgc tgcgcgaggg tgagctgaat gtcgccggtg ccgggcggca tgtcgacgac
      901 gagatagtcg agatcgcccc aatgcgtctg agtcagcaac tgattcaacg cggacgtcac
      961 cattggcccg cgccacgcca tcggctgctg ctcgtcgacg agaaagccga tcgacatcgc
     1021 ttccagcccg tgagcaacga gcggctcgag cgtcttaccg tcacgcgtct cgggacggcg
     1081 gcccgtgagc ccaagcatgc gcggctggct cggtccatag atgtccgcat cgagcaggcc
     1141 gacgcgcgca ccttcggccg cgagggccaa ggcgaagttc acggcgacgg tcgacttgcc
     1201 gacgccgccc ttgcccgacg cgaccgcgat caaattgtgg actccgggca gcggcttcag
     1261 gccgtgctgc acggcgtgcg agacgatggc actctcgatg ctgaactcga tggcggcgcc
     1321 ttcgagcgcc ggcgcacagt gcacgcgcag cgcttcgacg agacccgcgc tgctcgcggc
     1381 caacggaaaa ccgagccgga tcgtgacatg ccaaccggcg ccgcgccggt cgagctcgaa
     1441 cgacgcgccg gggccgccga gcggccggcc ggtctgcggc aggatgaagt cgccgagggc
     1501 tcgttggagg ttcgagggaa gggcttcggt cattacggag ggctcacgaa aggcggcgat
     1561 tgtaacctaa tgacggcgcg cggccggcgg cgagaaacca ctgtaacgtg gcgtgcaaac
     1621 ctgtgatacg cgttacattt cggcgaaaac cgtgtggcat aattgccgcc gtcggaagca
     1681 accattatgt tcattccggg cgttcaagga acggccggcc actggttcgt tgagtcaatt
     1741 ttggcgcgta taggacgacc gattctgcga ggccgatgaa cttcataacc gccttacagg
     1801 ccgatcggct gattgcgcag attcgcgagg aagcggaccc ttcctcggcc gacggcaaga
     1861 agctgttcgc caagctcggc aagctcggca ccggggccat acccaagatc ctcgaggccc
     1921 tcgcgtcggc cgacaagcgc caaacggtcg agtacgtcga gctgctgaat gcgctgatca
     1981 gcgacaagac cttgccgctg atcacgcgcg ggcttgccga cggcgacccg cgcacggtct
     2041 cgggcacggc ctgggcgctg tcgagcaaca agcggttcaa cgtcaaccgg ctcgtggacc
     2101 tgctcggcga ggacgactac tcgaaagccg cgatcgtcga ggtcctgcaa gcacacaagg
     2161 accggctcaa cgtccggcag ttgctggggc aaatctactt cctgcagccc agcgagaagg
     2221 gcgcggtctt caagctcatc gacgaggtca cgaccgagga catggtgcct gacctgctcg
     2281 cacgcatgga cggcaaggac ccgctggtca agacgcatct aatcaacgtg ctcgcgcgct
     2341 tcgacagccc cgaggtcaac aaggcactcc aggaagccct acgcgacagc aacaagctcg
     2401 tgcgcggcgc cgcgctggcc ggcatcgcgc gctcgaagac gacgctcgac gtggcgctcg
     2461 tggccgggct gctgctcgac cccgacctcg acgtcatgaa caaggccgtc gacgtcatca
     2521 tccagctgaa tcatccggaa acggcgaaat atttgatcga cgcgctgaag gccgagaacg
     2581 agttcagccg ccggtcggcc gtcgagatct tgaacgcgat cggcacgacg cacagcgtca
     2641 agtggctgct cgaggccgtc gccgacgagg attggtgggt gcgatcgcgc gcttcggacg
     2701 ccttggcgcg catcggcggc ccgcgcgtcg tcgacgccgt gctcgatctg atcaaggaca
     2761 aggacgagaa catccggcgc gcggcgatcg agatcctgaa cacgtgccgc gacaaacgcg
     2821 ccgtcgaccg gctcatcgat gccacgaagg acaaggactg gtgggtcagc gaacgcgccg
     2881 cggacgcatt ggccgagatc ggcgacgcaa aggcgctgcc agcgctgctc gagatgatgg
     2941 ccaagaacaa ccgctcgctg cccgtcgcaa tcgcggcgat cggcaagctg aataacccca
     3001 aggtgctcga caagatcctg ccgtacctgc agcggcccga gaaagaggtc cgcgtcgccg
     3061 caatcggcgc cgtcgtgcag ctcgccggcg agcagcaagc ggaagccgtg cggccccttc
     3121 tacaacagag cgcgcaaggc gccgacgaga ccgtcgtgcg cgccgtgacg agggcgctgc
     3181 aaaagctcga gggcaagctc tcgccgagcg gcaactacag gaacaacacg acggtcgtag
     3241 gcgctaccgt cgcaagctcg acggcgccgc cggcgccggc ggccactgtg acgcgagcag
     3301 cgacgacccg cgccgcaccg acgccccctc ctcccgcgcc gtccgggacg gcgacggcgc
     3361 ggacgttgct cgtcgaagga ccggacgacg cggtacccga gccgccgcca cccgaagcgc
     3421 cgtcgttcga tctgagcgca ttgcagcccg gcgacatgat cgagggacgc tacaagttcg
     3481 tgcagaagat cggcaaaggc gcattcggca ccgtcgtgct cgtcgaggac accgtcgtcg
     3541 aagagcggct gattctgaag ttcttgaacg cgaacgtggc ctccgacgaa gagatgctga
     3601 agcgcttcgt gcacgagctg cgctattcgc ggcgcatcac gcacaagaac gtcatccgca
     3661 tctacgactt cctgtacgtc ggcggcagtt atgcgatctc gatggaatac ttcccgtcgc
     3721 acacgctcgg cgcggaaatc gcgaacgaaa agcccatgcc gcccaagaag gccgtcggct
     3781 acggcaccga catctgtgtc ggcatggccg tcgcgcatca gcaaggcatc atccatcgcg
     3841 acttgaagcc cgcgaacatc ttgatcaacg aaacggggct cctgaagatc gtggacttcg
     3901 gcgtcgccgc ggccgccaag agcggcgaca cgcagctgac gaagaccggc tacgtcatcg
     3961 gctcgccgaa gtacatggcg cccgagcaga tcctcggcaa gaaggtcgac gagcgggccg
     4021 acatctacag cctcggcgtc atcatgtacg agatgctcgc cggcaacccg ccctacagcc
     4081 gcggagatca catgtcggtc atgtaccaac acgtgcaggg caaggcgcaa ccgctcaacg
     4141 aagcgaaccc taaggtcccg cgcgagatcg ccgacgtcgt cacgaagacc atgcaggtcg
     4201 acaagctgaa acgctatgcg tcgatggaag agctgcgcgt agcgctcgaa gctgcaacgg
     4261 ccgcggtcag gtaatgcaat ggcacgtatc gacgccttcc tgaagctcgg cctcgctcaa
     4321 ggctgctccg atattcactt ggccgtcggc atcccgccga tgctgcggtt gcacggcgat
     4381 ctcatgccga tcaagtttcg cgacttgggc gataccgagc tcgagggcta tgtcgacgaa
     4441 atcttgagcg actcgcaacg cctgcgcttt cgccagaaga acgacatcga tttttcctac
     4501 gtcagcaccg acggcggccg cttccgcgtc aacgtgttcc acaaggcgac cggtgtcggc
     4561 gcgacgttcc gcaacattcc cgccgtcgtc ccgagcctcg acagcctcgg tctgccgccg
     4621 atcctgcaca aattctgcga cctgcaccaa ggcatggtgc tcgtgacggg ctcgacgggt
     4681 accggcaagt cgacgacgct cgcggcgatg atcgatcacc tcaacacgac cagtaactcg
     4741 aacatcattt ctctcgagga tccgatcgag ttcgtgcact cgagcaagcg tagccagatc
     4801 atccagcgcg agctcggcac gcacatcccg agcttcgccg aaggcgtgcg cgccagcttg
     4861 cgcgaggacc ccgacgtcat cctcgtcggc gagctgcgcg acggcgagac gattagcatg
     4921 gccatgacgg ccgccgaaac cggccacttg gtcctcggca cgctgcacac gacgagcgcc
     4981 gtgaagtcga tcgaccgcat catcgacgcc ctgcccggcg accagcgcga gcaaacgaag
     5041 agctttctct cgaacagctt gcacgccgtc gtgacgcaga acctcatcaa gacgccggac
     5101 ggccgcggcc gccgggccgt cgtcgaggtc atggtcatga atcgggcgat cgcgaagctc
     5161 attcagaccg agcaaacgca tcagattccc gcgcagctgc aaaccggcaa ggacgccggc
     5221 atgcagctca tggatcaagc gttgctgaac gcgatcagcg ccaagctcat cgatcccgac
     5281 gacgcgtacc gctacgccac ggaccgcaag aagttcgagc ggttcgtcac cgataccacg
     5341 gtgctcccga gcggcgtgac gacgacgacg ccggccgggc aagcggccgg caagagcgcg
     5401 ggtacgggct gagcaggagc cgaagatgtc cctgatcgaa gacttcctgc gcagcatgta
     5461 cttgcgcaaa ggttcggacc tccatgtcat cgccggcgat ccgccgcgca tgcgcgtgca
     5521 cggcgatctc atcacgctcg acaaccagcg cctcgatccc gaggagctgc gctccgagct
     5581 gttcggcatc atgtcggagc aatcgctcga gcaattcaac aagcacgact cggcggactt
     5641 cgctcactac attcccgacg tggcgcgatt tcgcgtcaac gtcttccgcc atttgaacgg
     5701 cattggcgcg gtctttcggg gcattccgtc gaagtgcctg acgctcgaag agctcaagat
     5761 gccgaaagcg ttgcacgagc tcgcgacggc aactcgcggc ttgattctcg tgacggggaa
     5821 gaccggctcg ggcaagtcga cgacgctggc cggcgtcgtc gacgccatca acagcaagat
     5881 caaaggt
//
LOCUS       DATLEE010000361         3041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5628708, whole genome shotgun sequence.
ACCESSION   DATLEE010000361 DATLEE010000000
VERSION     DATLEE010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5628708"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            397..1815
                     /locus_tag="VM692_07795"
     CDS             397..1815
                     /locus_tag="VM692_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUQ52110.1"
                     /translation="MNVSKLAFLKSATIEADEAIRSKNAVVVASGTPGLGRRTLLLAA
                     ASSAGARILEVNSKKLSKDHSQLKKQLKLIARECKLLQRAPLICNIDALVDDKDQARV
                     DLVGSELVSEIDGPVFVTCGVQRPAMRWDRPCVVIDVKPPTSEQRAKLWRDSLGQGTE
                     EDSQFLATQYPLAPALIVRAAEAAKTRANGGKLQPEDIYAGVRAVLDDKLGDYAKRVD
                     VTQSWDDLVLPQDQLDAIKYLVARIRQRRTVYEQWGFAAKVGKGLGTSALFSGPPGTG
                     KTMVAALIAKELGLELYQVDMAKVTSKWIGETERNLAALFDAAEAGHAILLFDECDSL
                     FGKRTDVKSSNDRYANLETNYLLQRLESFTGICLLTTNHENAIDPAFMRRLSLHVRFE
                     LPEAAEREQLWRAMLPAAAPVAPSLDVAALARRFVMSGGYIRNAALRAAFVAADEGSA
                     ITTRHLECAAKLEYEGMGKIAA"
     gene            1874..2797
                     /gene="fabD"
                     /locus_tag="VM692_07800"
     CDS             1874..2797
                     /gene="fabD"
                     /locus_tag="VM692_07800"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HUQ52111.1"
                     /translation="MKTALLFPGQGSQRVGMGRDLAIAHEVAKRTYEEADDALGFAIS
                     KLCWDGPQDELTLTKNTQPAILTTSVAVFRTLLEKGLQVDVVAGHSLGEWSALVAAGA
                     LSLRDAVKLTHLRGTFMQEAVPVGQGAMAAIIGLDLEKARALCEKASAPGEPVEPANL
                     NGAGQIVISGHVNAVDRAVAEAKPSGAKMAKKLDVSAPFHCSLMKPAAEKLAAAMADV
                     VINTPNVPVVANVTAEPNTDRARIKELLVQQVTAPVRWEESIQCIAKMGVTRAYELGS
                     GAVLKGLVKRIADSIEVTTIGEPHEVAAFNA"
     gene            2807..>3041
                     /locus_tag="VM692_07805"
     CDS             2807..>3041
                     /locus_tag="VM692_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ52112.1"
                     /translation="MAIVDKALQGRVALVTGGSRGIGRAIAIALGSRGAKVVVNFASR
                     EDAARETAEAVKAAGGDAATLGFDVGNSAAVSDA"
BASE COUNT          546 a          871 c         1086 g          538 t
ORIGIN      
        1 gccgccgact gcgaacgagt gtctgactct tggtgtgccc gattgggtct cacatcgaca
       61 gagcagctcg tggtgtgggt gtgcgcggcg atcgccgtca gcgcgcaggc ccgagaggcg
      121 ctaggtgttc tggtgggcac cacaggcgat ccaacgttcc aaactctacg taccctcgtt
      181 tactcgaaga acgagcaacc agccgccctc acggagctcg gcccagaatg caagctagtc
      241 cggctcgggc tcctagagcg gtcggatgtc caccgtggtg atgctcacga agcaaatcac
      301 tcgtggcgcg ttgctgggag gatgctgctg ctgctttatg ggcagtgcat tctcgataca
      361 aaagcggccg acttcgcgaa agaggcgagc gaccatatga acgtttcgaa gctggcgttc
      421 ctcaagtcgg cgacgatcga agcggacgaa gcaattcgtt cgaagaacgc cgtcgtcgtg
      481 gcgagcggca cgccgggtct tggccgacgt acgctcctcc tagcggcggc ttcttcggca
      541 ggagctcgga ttctcgaagt caattcgaag aagctttcga aggaccactc gcagctcaag
      601 aagcagctga agctcatcgc tcgcgagtgc aagcttctcc agcgcgcgcc actcatctgc
      661 aacatcgacg cactcgtcga cgacaaagac caggcgcgcg tcgatcttgt tggcagcgag
      721 ctcgtttccg agatcgacgg ccccgtgttc gtcacctgcg gcgtgcagcg ccccgccatg
      781 cgctgggacc ggccttgcgt tgtcatcgac gtgaagccac ccacgagcga gcagcgcgcg
      841 aagctctggc gcgattcgct cgggcaaggc acggaagagg actcgcagtt tctggcgacg
      901 cagtatccac tcgcgcctgc actcattgtt cgcgcggccg aagccgcgaa gacacgcgcg
      961 aatggcggca agctgcagcc cgaggacatc tacgccggcg ttcgcgcggt gctcgacgac
     1021 aagctcggcg actatgccaa gcgcgtcgac gtcacgcaga gctgggacga cctcgtgctg
     1081 ccgcaggacc agctcgatgc gatcaagtat ctggtcgcgc ggattcgcca gcggcggacg
     1141 gtgtacgagc agtggggatt cgccgcgaag gtgggcaagg ggctggggac gagcgcgctg
     1201 ttttctgggc cgcctggcac aggcaagacg atggtggcgg cattgatcgc gaaggagctc
     1261 gggctcgagc tttatcaggt cgacatggca aaggtgactt cgaagtggat cggcgagacg
     1321 gagcgcaatc tcgctgcgtt gtttgacgcg gcggaagcgg gccacgccat tcttctgttt
     1381 gacgagtgcg actcgctctt tggtaagcgc accgatgtga agtcgagcaa cgatcggtat
     1441 gcgaacctcg agacgaatta tctgctgcag cgtctcgaga gcttcaccgg catttgtttg
     1501 ctgacgacca accacgagaa tgcgatcgat ccggcattca tgcgccgtct gtcccttcat
     1561 gtccgcttcg agctgcccga ggctgcggaa cgcgagcagc tgtggcgcgc gatgctgccg
     1621 gccgctgcgc ccgtggcgcc gagcctcgac gttgccgcgc tcgcgcggcg gttcgtgatg
     1681 agcggcggct acattcgcaa tgcggcgttg cgtgcggcct ttgtggcggc cgatgaaggc
     1741 tcggccatca cgacgcgtca tctcgagtgt gcggcgaagc tcgagtacga gggcatgggc
     1801 aagattgccg catgaacggg gtgacgcgcc tcgtcgttgc gtggtgattg cggtcgtgcg
     1861 atgagttggc gccatgaaga cggcattgct gtttcctggc cagggctcgc aacgcgtggg
     1921 catgggccgc gacctcgcca tcgcgcacga ggttgcgaag cggacctatg aggaggcgga
     1981 cgacgccctc gggtttgcga tcagcaagct gtgttgggat ggacctcagg atgagctgac
     2041 gctgacgaag aacacgcagc cggccatcct cacgacgagc gttgcggtgt tcaggacgct
     2101 cctcgagaag gggctgcagg ttgatgtcgt tgcggggcat agcctcggcg agtggagcgc
     2161 gctggttgca gcgggggcgc tgtcgctccg cgacgcggtg aagctcacgc acctgcgcgg
     2221 cacgttcatg caggaggcgg tgccggtcgg gcagggcgcc atggcggcga tcattgggct
     2281 agacctggag aaggcgcggg cgctttgtga gaaggcgagc gcgccggggg agccggtgga
     2341 gcccgcgaat ctcaacggtg ctggtcagat cgtgatcagc gggcatgtga acgcggtcga
     2401 tcgcgcggtc gcggaggcga aaccttccgg cgcgaagatg gcgaagaagc tcgacgtcag
     2461 cgcaccgttc cattgttcgc tcatgaagcc tgctgcggag aagcttgcgg cggcgatggc
     2521 ggatgttgtc atcaacacgc cgaacgtgcc ggtggtcgcc aacgtgacgg cggagccgaa
     2581 cacggacagg gcgcgcatca aggagctgct tgttcagcag gtgactgcgc ctgtgcgctg
     2641 ggaagagtcg atccagtgca tcgcgaagat gggcgtgacg cgggcgtacg agctcggtag
     2701 cggggccgtg ttgaagggcc tcgtgaagcg cattgccgac agcattgaag tcaccaccat
     2761 tggcgagcca cacgaagtgg ccgcgttcaa cgcgtaggag agcaagatgg cgatcgtcga
     2821 taaggcattg caggggcgcg tcgcgctcgt cacgggtggc tcgcgcggca ttgggcgggc
     2881 gattgcgatc gcgctcggta gccgcggggc gaaggtggtc gtgaattttg cgtcgcgcga
     2941 ggatgcggcg cgcgagaccg cggaggctgt gaaggcggcc ggcggcgatg cagcgacgct
     3001 cgggtttgat gtcgggaaca gcgcggcggt cagcgatgcc g
//
LOCUS       DATLEE010000362         2620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1157854, whole genome shotgun sequence.
ACCESSION   DATLEE010000362 DATLEE010000000
VERSION     DATLEE010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157854"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VM692_07810"
     CDS             <1..1084
                     /locus_tag="VM692_07810"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HUQ52113.1"
                     /translation="GKAYFVRAGVFADVDAVLYAHVNSVLATEWGDADTSSAVSVEYT
                     FTGQSAHAARAPWLGRSALDAVELMNVGWNYRREHLRLAQRSHYVVSNGGDQPNVVPP
                     LASVWYYFRENDFAHVQDLWTIGNQMAQGAALMTGTEFTSRVLGSAWPHHRNRPLAEA
                     IQRNIELVGLPQWSDADQQFARAVQRSLGVPERGLVTELRELRGVEQMRDEDKSGSAS
                     DDIGDVMWNAPTAFLGYPANIGGTTAHNWTAAIAMATPVAHKGTAQGAMVYAATIVDL
                     ALRPELVAAAHDYFANVQGKLATYTPFIRETDEPVVQMNKETMDRYRPELAKHYYDAR
                     KFDNYLEQLGVAYPPALPPAPAAPSR"
     gene            complement(1094..1657)
                     /locus_tag="VM692_07815"
     CDS             complement(1094..1657)
                     /locus_tag="VM692_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HUQ52114.1"
                     /translation="MESWPQRHRITVHDYHRMAEIGVLAPDARVELIEGEIIDTAPIG
                     NDHQSVVDQLTRLLVRAVGDAAIVRVQGSIRLGQWSEPEPDVVLLAPRADFYRSEFAL
                     GTDTLLVIEVSDTTLRYDRDVKVPLYARPGVPEVWVVDLNSATLLVYDGLRAGKYERH
                     VALERPASMPVTRLPGVMLDVAALFGS"
     gene            complement(1792..>2620)
                     /locus_tag="VM692_07820"
     CDS             complement(1792..>2620)
                     /locus_tag="VM692_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ52115.1"
                     /translation="DVTIFSPRASWMGHHLGNASTSKAWSEHCNDLIYRLTKLYPKNF
                     VGVAQLPQSPGAPIDSSVRELVRCVEQLGFVGCNLNPDTSGGFFNGPGLADRYWYPLY
                     EKMCELQVPAMIHVSEACNTPYFQTTGSYYLAADTVAFMQMMTSDLFKDFPSLKLIIP
                     HGGGAVPYHWGRFRGLADMLKRPPLKESVLNNVYFDTCVYHQPGIDLLLDVIPIDNIL
                     FASEMVGAVRGIDPETGHYFDDTKRYIDGSKKLSAADKTKIFEGNTRRVFPRFPAAL"
BASE COUNT          475 a          858 c          848 g          439 t
ORIGIN      
        1 cggcaaagcg tacttcgtcc gcgcgggagt gttcgccgac gtcgatgccg ttctctacgc
       61 ccacgtcaac tcagtgcttg ccacggaatg gggagacgcc gacacgagca gcgccgtatc
      121 cgtcgagtac acgtttacgg gccagagcgc acatgcggca cgcgcgccgt ggctcggccg
      181 tagcgcgctc gacgccgtcg agctcatgaa cgtcggctgg aactaccggc gcgagcattt
      241 gcgactcgcg cagcgctcgc actacgtcgt cagcaacggc ggcgaccagc cgaacgtcgt
      301 gccgccgctg gcctccgtct ggtactactt ccgcgagaac gatttcgcgc acgtgcaaga
      361 tctctggacg atcggcaacc agatggcgca aggcgccgcg ctgatgacgg gcaccgagtt
      421 cacgtcgcgc gtgctcggtt ctgcgtggcc gcatcatcgg aatcgcccgc tcgcggaagc
      481 cattcagcgc aacatcgagc tcgtcggcct gccgcagtgg agcgacgccg atcagcagtt
      541 cgcgcgcgcc gtgcagcgct cgttgggcgt gccggaacgc ggtctcgtga cggagctccg
      601 cgagctgcgc ggcgtcgagc aaatgcggga cgaagataag agcggcagcg cgtcggacga
      661 catcggcgac gtcatgtgga acgctccgac ggcgttctta ggctatcccg ccaacattgg
      721 cggcacgacg gcgcacaatt ggacggccgc gattgcgatg gcaacgcccg tcgcgcacaa
      781 gggcacggcg cagggtgcga tggtttacgc ggcgacgatc gtcgatctcg ctctgcgccc
      841 cgagctcgtc gccgccgccc acgactactt cgctaacgtg caaggcaagc tcgcaactta
      901 caccccgttc attcgtgaaa ccgacgagcc cgtcgtgcaa atgaacaagg aaacgatgga
      961 ccgctaccgt cccgagctcg caaagcacta ttacgacgcg cgcaagttcg acaattatct
     1021 cgagcagctc ggcgtcgcct acccgccggc gctgccgccc gctccggcgg cgccttcgcg
     1081 ctgagcggcg gcgctacgag ccgaacagcg cggccacgtc gagcatgaca cccggcaacc
     1141 gcgtcacggg catgctcgcg ggccgctcga gcgcgacgtg ccgctcatac ttgcccgcgc
     1201 gcaggccgtc atagacgagc agcgttgcgc tattcaagtc gacgacccag acctcgggca
     1261 cgccaggccg cgcgtagagc gggaccttga cgtcgcgatc gtagcgcagc gtcgtatcgc
     1321 tgacctcgat gacgagcagc gtgtccgtcc cgagcgcgaa ctcgctgcgg tagaaatccg
     1381 cgcgcggtgc gagcaggacg acgtcgggct cgggctccga ccactgcccc aatcggatcg
     1441 atccctgcac gcgcacgatc gcagcgtcgc cgacggccct gaccaatagc cgggtcagct
     1501 ggtcgacgac gctctggtga tcgttgccga tcggcgccgt gtcgatgatc tctccctcga
     1561 tcaactcgac gcgcgcatcc ggcgcgagca cgccgatctc cgccatgcgg tgatagtcgt
     1621 gcaccgtgat gcgatgccgc tgcggccagc tttccatgtg catgcccatc gtgctccggg
     1681 gccaacagtg taagggcggc gcaagcgtcg attgcaagct tcgcggcgcg gtctccggaa
     1741 ggcatgcagc gaagctaacg gaaacgatcg gtttcgcggc tcgcgacggg ctcagagtgc
     1801 cgcggggaat ctcgggaaca cgcggcgcgt gttgccctcg aagatcttcg tcttgtcggc
     1861 agcactgagc ttcttgctgc cgtcgatgta gcgcttcgtg tcgtcgaagt agtgcccggt
     1921 ctccggatcg atgccgcgca cggcgccgac catctccgac gcgaacagga tgttatcgat
     1981 cgggatgacg tcgagcagca agtcgatgcc gggctggtgg tagacgcacg tgtcgaagta
     2041 aacgttgttc agcaccgact ccttgagcgg cgggcgcttc agcatgtcgg cgaggccgcg
     2101 gaaccggccc cagtgatacg gcacggcgcc gccgccgtgc ggaatgatga gtttcaacga
     2161 cggaaagtcc ttgaaaagat cgctcgtcat catctgcatg aacgcgaccg tgtcggcggc
     2221 cagatagtac gagcccgttg tctggaagta cggcgtgttg caagcctcgc tgacatgaat
     2281 catcgccggc acttgaagct cgcacatctt ctcgtagagc ggataccaat agcggtccgc
     2341 cagcccgggg ccgttgaaga agccgccgct cgtgtcggga ttcaaattgc agccgacgaa
     2401 accgagctgc tcgacgcagc gaacgagctc gcgaaccgag gagtcgatcg gcgcacccgg
     2461 cgactgcggc aactgtgcga cgcctacgaa attcttcgga tacagcttcg tgagccggta
     2521 gatgaggtcg ttgcagtgct cgctccacgc tttgctcgtc gacgcgttgc cgaggtgatg
     2581 acccatccag ctcgcgcgcg ggctgaagat cgtgacgtcg
//
LOCUS       DATLEE010000363         3351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9353224, whole genome shotgun sequence.
ACCESSION   DATLEE010000363 DATLEE010000000
VERSION     DATLEE010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9353224"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VM692_07825"
     CDS             complement(<1..1352)
                     /locus_tag="VM692_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUQ52116.1"
                     /translation="MAPDLPQRLAAILAADISSYTRLMELDEAGTVAAWRRARDETID
                     PTIARFGGRIVKLTGDGFLAEFSTVESAVRAALAMQVELAAMFAAEPSDRRVAFRMGV
                     HFGDIFVDDEDVYGTGVNIAARLESLATPGGLCISDAVYAAINHKIDAHYEDLGQQRV
                     KNLASPVHVWRASAAARPLPARRAPKLRRFAIAAVAFVAIAAAAIYFGRTTLPPTENV
                     GAEESGAATNVARDVESNTIAVLPLLNIDGSDETRIFADGLAEDLINLLTATPPLRVS
                     SRGDSFSLGPNTEPQHVRDRLRVAYFVEGSVRRSADTLRVVIQLIDSASGFHIVSRPV
                     DRPVGEFLQLQDEIAKLIVANLRVAIPSLAQEPVYSTAESASFDAYLAYRRGMDILAK
                     PLTRAAVTEALSAFDESLTVDRDYAAAFAGICLANIAGYDTTQEAAYMANAERSCSSA
                     "
     gene            complement(1456..2190)
                     /locus_tag="VM692_07830"
     CDS             complement(1456..2190)
                     /locus_tag="VM692_07830"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ52117.1"
                     /translation="MPALSLVVVLQIIAVVHLFRTGRNMTWLFLIILVPLIGSIAYFI
                     VEVLPSLQNSPGARRALRRAQTAMDPGRDLRENTLNYERSQNVDTASRLAGELTKAGR
                     YDEAIQLCNAARTGLFEDDPKILLALSSAQFAASDFAGAITTLDYLREKNPGFRSADG
                     HLIYARALEESGATQRALEEYVALAAYYPGAEARVRQALLHKKLGDNARAAELFAALL
                     TDARLAPKHFRTSQKEWIELAERESN"
     gene            2300..2929
                     /locus_tag="VM692_07835"
     CDS             2300..2929
                     /locus_tag="VM692_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HUQ52118.1"
                     /translation="MIDLYTWTTPNGRKVSIMLEEVGLPYRAIAIDINANEQFKPEFL
                     AISPNNKIPAIVDHDNGTSLMESGAILLYLADKTGRLRGAGKNGWQAVEWLMMQMGSV
                     GPMLGQAHHFLKFNRGKSEYAEKRYGDEAQRIYGVLNKRLGDAEYLAGDYSIADIATW
                     PWIARYEWQGIDFALYPNLKRWYLEIAKRPAVQRGYQVPKEVSPISLPA"
     gene            complement(2904..>3351)
                     /locus_tag="VM692_07840"
     CDS             complement(2904..>3351)
                     /locus_tag="VM692_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HUQ52119.1"
                     /translation="EPRHAPLACEEARRVGLPFFIGVSCRIGAGDALVGFDFPLVPLA
                     ACLDALLPFAPTAINVMHTPLAAVEPALREIRARWHGPLGAYPEIGDGVPHAQHSVSP
                     DSLAAQACGWLANGARIIGGCCGTTPEHVGALARLPHSAKPVTKSG"
BASE COUNT          588 a         1072 c         1126 g          565 t
ORIGIN      
        1 atggcggagc tgcacgaacg ctcggcgttc gccatgtacg ccgcttcctg cgtcgtgtcg
       61 tacccggcga tgttcgcgag gcaaataccc gcgaacgcgg ccgcatagtc gcgatcgacg
      121 gtcagcgatt cgtcgaacgc gctcagcgct tcggtcacgg ccgcccgtgt caacggcttg
      181 gcgaggatgt ccatgccgcg gcggtacgcg aggtacgcat cgaatgacgc gctctccgct
      241 gtggagtaaa cgggctcctg ggcgagcgac ggaattgcga cgcgcaggtt agcgacgatc
      301 aacttcgcta tctcgtcttg cagctgcagg aactcgccga ccggcctgtc gacgggtcgc
      361 gacacgatgt gaaagccgct cgcggaatcg atcagctgaa tcaccacgcg cagcgtgtcg
      421 gcgcttctgc gcacgctgcc ctcgacgaag taggcgacgc gcaggcggtc gcgcacgtgt
      481 tgcggctccg tgttcggccc gagcgaaaat gagtcgccgc gcgacgacac gcgcaggggc
      541 ggcgtagccg tcaggagatt gatcagatct tcggcgagac cgtccgcgaa gatccgcgtc
      601 tcgtcgctgc cgtcgatgtt cagcagcggc aacacggcaa tcgtattcga ctccacgtcg
      661 cgtgcgacgt tcgtagcggc ccccgactct tcggcgccta cgttttcagt gggtggcaag
      721 gtcgtgcgtc cgaagtagat cgccgccgct gcgatcgcga cgaaggcgac ggctgcgatc
      781 gcgaagcgcc gcagcttcgg agctcggcgc gccggcagcg gccgagccgc ggcgctcgcg
      841 cgccagacgt gcacgggcga cgcgaggttc ttgacgcgct gttgtccgag atcttcgtag
      901 tgcgcgtcga tcttgtgatt gattgcggca tagacggcgt cggagatgca aagcccgccc
      961 ggcgttgcaa gcgactcgag cctagccgcg atgttgacgc cggtgccgta gacgtcctcg
     1021 tcgtcgacga agatgtcgcc gaagtgcacg cccatgcgga aggcgacgcg ccggtcgctg
     1081 ggctcggcgg cgaacatcgc cgcaagctcg acctgcatcg cgagcgcggc gcgcacggcg
     1141 ctttcgaccg tcgagaattc cgcgaggaag ccgtcgcccg tgagcttgac gatgcggccg
     1201 ccgaagcgcg cgatcgtcgg gtcgatggtt tcatcacggg cgcgtcgcca cgccgccacg
     1261 gtaccggcct cgtcgagctc catgaggcgg gtatacgacg agatgtcggc cgccaggatg
     1321 gcagcgagtc gttgcggaag atctggcgcc actatcgggc cctttgccga gcaacgcggg
     1381 cggggctaac tatagtccat ttctgccggc gcggtcattg ccggaacggc agcaatcggg
     1441 cgccaaaatg gggcgttagt tgctctcgcg ctccgcgagc tcgatccact ctttttgact
     1501 cgtgcggaaa tgcttcggtg caaggcgcgc gtccgtgagt agcgcggcga acagctccgc
     1561 cgcacgagcg ttatcgccga gcttcttgtg cagcagcgct tggcgcacgc gcgcttcggc
     1621 gccggggtaa tacgccgcaa gcgcgacgta ctcctcgagc gcacgctgcg tcgcgccgct
     1681 ctcctcgagc gcgcgcgcat agatcaggtg gccgtcggcc gagcggaagc ctgggttttt
     1741 ctcgcgcaag tagtcgagcg tcgtgatggc gccggcaaag tcgctcgctg cgaattgcgc
     1801 agagctcagc gccagcagga tcttcggatc gtcctcgaac agcccggtgc gcgccgcatt
     1861 gcaaagttgg atcgcctcgt cgtaacggcc ggccttcgtg agctcccctg cgagcctact
     1921 agccgtatcg acgttttgac tgcgctcgta gttcaacgtg ttctcgcgca agtcgcggcc
     1981 gggatccatt gcggtttgtg cgcgcctcag cgcacgccgc gcgcctgggc tgttctgcag
     2041 cgatggcagc acctcgacga tgaaatacgc gatcgagccg atcagcggca ccaggatgat
     2101 caggaacagc caagtcatgt tgcggccggt gcggaacaga tgcacgacgg cgatgatctg
     2161 cagcacgacg acgagcgaca gggcgggcat gggtctcgac ctcgcgaacg tggcgcctat
     2221 gattctgtgg cgctgggtta ctcgcaaggg cgtaggtcgc taaaattgcg cccttcgaat
     2281 ccccggagca cgcgcgctcg tgatcgacct ttatacctgg acgacaccga acggccgcaa
     2341 ggtctcgatc atgctcgagg aagtcggcct gccgtaccgg gcgatcgcga tcgacatcaa
     2401 tgccaacgag caattcaaac cggagtttct cgcgatcagc ccgaacaaca agatccccgc
     2461 gatcgtcgac cacgacaatg gcacatcgct gatggagtcg ggcgctatct tgctgtatct
     2521 cgccgacaag acgggccggc tgcgaggcgc gggcaagaac ggctggcaag ccgtcgagtg
     2581 gctcatgatg cagatgggca gcgtgggccc gatgctcggc caagcgcatc acttcttgaa
     2641 gttcaaccgc ggcaagtccg aatacgccga gaagcgctac ggcgacgaag cgcagcgcat
     2701 ctacggcgtg ctgaacaaac gcctcggcga cgccgagtat ctcgccggcg attactcgat
     2761 cgccgacatc gcgacctggc cgtggatcgc gcgttacgaa tggcagggca tcgacttcgc
     2821 gctgtatccg aacctgaagc gctggtatct cgagatcgcg aagcgacctg ccgtgcagcg
     2881 cggctatcag gtgccgaagg aagtcagccc gatttcgtta ccggcttagc cgagtgcggc
     2941 agtcgcgcca atgcgcccac gtgctccggc gtcgtgccgc agcagccccc aatgattcgt
     3001 gcgccgttcg cgagccagcc gcacgcctgc gccgcaaggc tgtccgggct cacggagtgc
     3061 tgcgcgtgcg gaacgccgtc gccgatctcg ggataggcgc cgagcggtcc gtgccagcgt
     3121 gcccgtatct cgcgcaaggc cggctcgacg gcggccaggg gagtgtgcat gacgttgatg
     3181 gccgtcggcg cgaacggcag cagcgcatcg agacaagctg ccaacgggac gagcggaaag
     3241 tcgaagccga cgagcgcgtc gcctgcgccg atgcgacaac tgactccgat gaagaagggc
     3301 agtccgacgc gacgcgcctc ttcgcaggcg agcggcgcat gccgcggctc c
//
LOCUS       DATLEE010000364         3046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2650535, whole genome shotgun sequence.
ACCESSION   DATLEE010000364 DATLEE010000000
VERSION     DATLEE010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2650535"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VM692_07845"
     CDS             <1..474
                     /locus_tag="VM692_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ52120.1"
                     /translation="RRMAAHLSDLERLQIRVALEDLNTAFCYHLDHNEVDALLDLFVD
                     DVNYTHGSRVSRSKAELAEVFRGRGATQVRTARHMYSGLRLDIDSPTRARGTSVCMTF
                     GQYGEPPLSPAIPTLVADFVDAYVRGDDGRWRFKERHIHRIFVAPDNTGPIGQKK"
     gene            complement(478..798)
                     /locus_tag="VM692_07850"
     CDS             complement(478..798)
                     /locus_tag="VM692_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52121.1"
                     /translation="MQWKPIIQGLAVVWLMLFVFSFVSLRTAATDDAETSDLTRIVAF
                     LTWQAIAFVVAAFGALTTRLALERGVAGIKLAGYLPLALSVFLVASFVALMGFRFYVT
                     PLFE"
     gene            complement(808..1665)
                     /locus_tag="VM692_07855"
     CDS             complement(808..1665)
                     /locus_tag="VM692_07855"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HUQ52122.1"
                     /translation="MARMPSLFVSHGSPLLAVVDSAARRFLQELGPRLPAPTSIVAIS
                     AHYDTPTAEITAAAQPETIHDFGGFPAELYRLRYPAPGNPELARAIADRLNAAGVPAN
                     VEPRRGLDHGAWIPLALMFPRADVPVLQVSIGTRRSPEQHFALGRALRSLRDTGALIL
                     GSGGATHNLALYAHARGRTDDSAPPEWVEAFNEWTAGAIAARRFDDLFRYAELAPYAA
                     QNHPTPDHFLPLFVTLGAAHDDEPSARIHTSYDRGLLSLDAYAFGLAAQTSGEIRVRA
                     AAQSSTVLH"
     gene            1710..2600
                     /locus_tag="VM692_07860"
     CDS             1710..2600
                     /locus_tag="VM692_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HUQ52123.1"
                     /translation="MRQERSEALVAPVELTIQPRVRSLGEFDVRRVLPAAKRRMVGPF
                     VFLDHMGPAVFPPGHGIAVRPHPHIGLATITYLFEGEIMHRDSLGYRQLIQAGAVNLM
                     TAGSGIVHSERASDDLNQTSRLHGIQSWIALPRELEETEPTFLHYPAMTLPERSIDGC
                     TVRVIMGSAYGVQSPVLTYSPTLYFEATLPADRTLVLLDEAQERAVYVVAGSVAIGAD
                     VYGEGTLAVLHEGARAPLRAQADTRVIVVGGAPVGPRHIWWNFVSSSEARIERAKRDW
                     AEMRMGTVPGDDDFIPLPDA"
     gene            2694..>3046
                     /locus_tag="VM692_07865"
     CDS             2694..>3046
                     /locus_tag="VM692_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019094101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUQ52124.1"
                     /translation="MTTRAFFDPFGAFAREEVTSTPRSTERSPHSYPALRSARSRANA
                     KRILVTGATGFIGTKLVHRLIERGDDVLVLARNPAKAADLFGPCAEVVTSLRSVATAT
                     RIDAIVNLAGESIAGG"
BASE COUNT          552 a         1015 c         1010 g          469 t
ORIGIN      
        1 cggcgcatgg cggctcacct gagcgacctc gagcggctac agatcagggt cgcgctcgag
       61 gatttgaaca cggcgttctg ctaccacctc gatcacaacg aggtcgacgc gttgctcgac
      121 ctgttcgtcg acgacgtcaa ctacacgcac ggcagccgcg tctcgcgcag caaagccgag
      181 ctcgcggaag tattccgcgg ccgcggcgcg acgcaggtac gcacggcgag gcacatgtac
      241 tcggggcttc ggctcgacat cgacagcccg acgcgggcac gtggcacgtc cgtctgcatg
      301 acattcggtc agtacggcga gccgccgctg tcgcccgcga ttcccaccct cgtcgccgac
      361 ttcgtcgatg cgtacgtgcg cggcgacgac ggccggtggc gcttcaagga gcggcacatc
      421 caccgcatct tcgtcgcgcc cgacaacacg ggcccgatcg gccaaaaaaa gtgagcgtca
      481 ctcgaacaac ggcgtgacgt agaagcggaa ccccatcagc gcgacgaacg acgcgacgag
      541 aaagacgctc aatgccagcg gcaagtagcc ggcgagcttg atgccggcga cgccgcgctc
      601 gagcgcgagg cgcgtcgtca atgcgccgaa cgcggcgacg acgaacgcaa tcgcttgcca
      661 cgtcaggaac gcgacgattc gcgtgaggtc gctcgtctcg gcgtcgtccg tcgccgccgt
      721 ccgcagcgag acgaacgaga agacgaacag catcagccaa accacggcga gcccttggat
      781 gatcggcttc cattgcatgc gcgttaccta gtggagaacc gtggagctct gcgccgccgc
      841 tcgcacacga atctcgccag acgtttgcgc cgcgaggccg aacgcatagg catcgagcga
      901 cagcaagccg cgatcgtagc tcgtgtgaat tctcgcgctg ggctcgtcgt catgagcggc
      961 gccgagcgtt acgaacaagg gcaggaagtg atccggcgtc gggtgattct gcgccgcata
     1021 cggtgcgagc tcggcgtagc gaaacaagtc gtcgaagcgc cgggccgcga tggctcccgc
     1081 ggtccactcg ttgaacgctt cgacccattc cggcggcgcg ctgtcgtccg ttcgcccgcg
     1141 cgcatgtgcg tagagcgcga gattatgtgt ggcgccgccg gagccgagga tcagcgcgcc
     1201 cgtatcgcgc aacgaacgca gcgcgcggcc gagtgcgaag tgctgctccg gcgaacggcg
     1261 agtgccgatc gacacctgca gcaccggcac gtcggcgcgc gggaacatca gcgcaagcgg
     1321 gatccaggcg ccgtgatcga gaccgcgacg cggctcgacg ttcgcgggaa ctccggccgc
     1381 gttcaggcgg tctgcaatag ctcgcgcgag ctccggattg ccgggcgccg gatagcgcaa
     1441 gcgatagagc tcggccggaa agccgccgaa atcgtgaatc gtctcgggtt gcgcggcggc
     1501 ggtgatctcg gccgtcggcg tatcgtagtg cgccgagatg gccacgatcg acgtgggcgc
     1561 cggcaatcgc ggacccagct cttgcaggaa tcggcgggct gcgctgtcca cgacggcgag
     1621 cagcggcgag ccgtgcgaga cgaacaacga aggcattcgg gccatgtgcc attatgccct
     1681 gccgtaggga ccaggttgag gaggccgacg tgagacaaga acgcagtgaa gcgctcgtcg
     1741 cacccgtaga gctcacgatc caaccgcgcg tgcgcagcct cggcgagttc gacgtccgac
     1801 gtgtgctgcc cgcggccaag cgccgcatgg tcgggccgtt cgtgttcctc gaccacatgg
     1861 ggcccgccgt cttcccgccg gggcacggca tcgccgtgcg cccacatccg cacatcggcc
     1921 tcgcgacgat cacctatctg ttcgaaggcg agatcatgca tcgcgacagc ttgggctatc
     1981 ggcaactgat ccaagccggc gccgtgaacc tcatgacggc cggcagcgga atcgttcatt
     2041 cggagcgcgc gagcgacgat ctgaaccaaa cgtcgcgcct gcacggcatt cagtcgtgga
     2101 ttgcattgcc gcgcgagctc gaggaaaccg agccgacgtt tctgcattat ccggcaatga
     2161 cgctacccga gcgcagcatc gacggctgca cggtgcgcgt aatcatgggc agcgcctacg
     2221 gcgtgcagtc gcccgtgctg acgtactcgc cgacgttgta cttcgaagcg acgctgccgg
     2281 ccgaccgcac gctcgtgcta ctggacgaag cgcaagaacg cgccgtctat gtcgtcgcag
     2341 gcagcgtggc catcggcgcc gacgtgtacg gcgaaggaac gctcgccgtg ctgcacgaag
     2401 gcgcgagggc gccgctgcgc gcgcaagccg acacgcgggt catcgtcgtc gggggcgcgc
     2461 ccgtcggccc acggcatatc tggtggaact tcgtctcgag ctcggaagcg cgcatcgagc
     2521 gcgccaagcg cgactgggcc gaaatgcgta tgggcacggt gcccggcgac gacgatttca
     2581 ttccactgcc ggacgcctag ccgccgcgcc gtcatcgagc gactgacggc gttgacagcc
     2641 caaccgtttg ggcctatcgt aatttctgta aatacagaaa ttactgatta tccatgacga
     2701 cgcgagcttt cttcgatcca ttcggcgcgt tcgcccgcga ggaggtgacg tcgacaccgc
     2761 gcagcaccga gcggtcgccg cactcttacc ccgccttacg ctcggcgcgg tcgcgcgcca
     2821 acgccaagcg catcctggtg acgggcgcga cgggtttcat cggcacgaag ctcgtccatc
     2881 ggctcattga gcgcggcgac gacgtgctcg tcctcgcgcg caaccctgcg aaagccgccg
     2941 acctattcgg tccgtgtgcc gaagtcgtga cgagcttgcg gtccgtcgcg acggcgactc
     3001 gcattgacgc gatcgtgaac ctcgccggcg agtcgatcgc cggcgg
//
LOCUS       DATLEE010000365         2823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_415972, whole genome shotgun sequence.
ACCESSION   DATLEE010000365 DATLEE010000000
VERSION     DATLEE010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415972"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VM692_07870"
     CDS             complement(<1..504)
                     /locus_tag="VM692_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52125.1"
                     /translation="MTAGFRQDVHYALRALASRPLYALASVAVLAIAIGANSTVFSVF
                     NGLFLRPLPYPDGDRLVMVYDSYPKQNLQNAGTAIPDYLERRAQAPSLENLAIVAEQP
                     RTLASDGPPQRLQVARVSPSLFDVLRVRPAIGRSFSDDEATLGNEFVAILSYRLWSGR
                     FAARRDIV"
     gene            complement(517..>2823)
                     /locus_tag="VM692_07875"
     CDS             complement(517..>2823)
                     /locus_tag="VM692_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52126.1"
                     /translation="GANSAVFSAIDAILLQPLGFPDADRLVVVNQSIPNAPVSNTAPV
                     RIEEWNEQSTAFAAITGYYREDVSETSGDLPEKYRRAHVAPRFLDVWRTKPLIGRAFT
                     PADNQAGAAPVALISHRYWTTKLESDPNVLERTVRIGEQAIPIVGVMPPSFRFPDRDV
                     DIFTPLAYEPYVLNRRNLWMRGYGRLEPGVSIEQARADLELVQGRLTQQYPETDRDTG
                     VYVEQLKETTVGAVRGSLWLLFGAVSLLLLIASTNIAALLLARAAKRSQEISVRLSLG
                     ASSASIRSQILVETALLAVVGALLGLLVAAGAAGALRRLTPDFPRIEELRLDGGILLY
                     TLGAIVAVTLICGLLPAVRAARVSLSGALTDARRTQVSGRHSLQWVFVGIQVALSVVL
                     LAGAGLLIRSFHELSRVDPGFEASRVLSFRIGGTFADFGGLADNVGRILDELGALPGV
                     EATAVSSPVPGMIDDGSGFQFGIASLTIDGRDATQQDPARAQIRVVSPSYFATMQIPL
                     LGGDLCRVIPPNTPDTPVEVMINAAFATRYLGSVSAAVGAIVRPAPNVALRVAGVVGD
                     AREFALDRAPVPSYYPCRLAYATPALAFLVRSRGDPANVTDVVRAKVKELEPLRAVYD
                     VAPLAERIGNEHAQDRLRTSALALFAGTALALACLGVYGTLSYVVSLRRREVGLRVAL
                     GAQPGNIVAQFLSKALRVVAVAAAAGLVLSLVFARALAGMLFGVSPFDAVTFGGVVAL
                     VTSVAIVAALVPAVRAARVDPMTVLRDE"
BASE COUNT          513 a          963 c          958 g          389 t
ORIGIN      
        1 gacgatgtcg cgccgagccg cgaaccgccc gctccagagt cgatagctca agatcgcgac
       61 gaactcgttg ccgagcgtcg cttcgtcgtc gctaaagctg cgcccgatgg cgggccgcac
      121 gcgcagcacg tcgaacaacg acggcgatac gcgcgcgacc tgcagccgtt gcggcggccc
      181 gtcggacgcg agcgtgcgcg gctgctcggc gacgatcgcc agattctcga gcgatggcgc
      241 ctgcgcgcgc cgctcgaggt aatccgggat cgccgtgccg gcgttctgca ggttctgctt
      301 cggataagag tcgtacacca tgacgagccg atcgccgtcc gggtacggta acggccgcag
      361 gaataagccg ttaaacacgc tgaacacggt cgagttcgcg ccgatcgcga tggcaagcac
      421 cgcgaccgag gccagcgcgt agagcggccg gctcgcgagc gctcgcagcg cgtagtggac
      481 gtcctgtcgg aaaccagcgg tcatggcgac acctcgttat tcatccctga gcacggtcat
      541 tggatcgacg cgcgcggcgc gcacggccgg cacgagcgcc gcgacaatcg caacgctcgt
      601 cacgagcgca acgacgccgc cgaacgtgac ggcgtcgaac ggcgatacgc cgaacaacat
      661 cccggcaagg gcgcgcgcga acacgagcga cagcacgagt ccagccgccg ccgccacggc
      721 gacgacgcgc agcgctttgc tcaagaattg cgcgacgatg ttgcccggct gcgcgccgag
      781 cgccacgcgc aagccgacct cgcggcggcg caagctgacg acgtagctca acgtgccgta
      841 aacgcccaag caggcaagcg ccagtgccgt gccggcgaac agcgcgagtg ccgacgtccg
      901 caagcgatct tgcgcatgct cgttgccgat gcgctctgcg agcggtgcga cgtcatagac
      961 ggcgcgcagc ggctcgagct ccttgacctt ggcgcgaacg acatccgtaa cgttcgccgg
     1021 gtcgccgcgc gagcgcacga ggaacgcgag cgctggcgtc gcgtatgcca ggcggcaagg
     1081 gtagtacgac ggcacgggcg ctcgatcgag cgcgaactcg cgcgcgtcgc cgacgacgcc
     1141 cgcgacgcgc agcgcaacat tgggcgccgg ccgcacgatc gcgccgacgg cggccgacac
     1201 actgccgaga tagcgcgtcg cgaacgcggc gttgatcatg acctcgactg gcgtgtccgg
     1261 cgtgttcggt gggatgactc gacagaggtc gccgccaagc aacgggatct gcatcgtcgc
     1321 gaaatagctc ggcgacacga cgcgaatctg cgccctggcc gggtcctgtt gcgtcgcgtc
     1381 gcgcccgtcg atcgtcaggc tggcaatgcc gaactgaaat ccggagccgt cgtcgatcat
     1441 gcctggcaca ggcgacgaga cggccgtggc ttcgacgccg ggcagagccc cgagctcgtc
     1501 gagaatgcgg ccgacgttgt ccgcgaggcc gccgaaatcg gcgaacgtgc cgccgatgcg
     1561 gaagctcaac acgcggctcg cctcgaagcc gggatcgaca cgcgacagct catggaagct
     1621 gcggatcagc aagccggcgc cggccagcag cacgacggac aacgcgactt ggatgccgac
     1681 gaacacccat tgcagcgagt ggcggcccga cacttgtgtg cggcgcgcgt cggtcagcgc
     1741 gccgctcaag ctgacgcgcg cggcgcggac cgccggcaag agcccgcaga tcagcgtcac
     1801 ggcgacgatc gcgccgagcg tgtagagcag gatgccgccg tcgagccgca gctcctcgat
     1861 acgcgggaaa tcgggcgtga gcctgcgcag cgcgccggct gcgccggcgg ccacgagcaa
     1921 cccgagcagc gcgccgacga ccgccagcag tgcggtttcg actaagatct gcgacctgat
     1981 cgacgccgac gatgcaccga gcgacaaccg cacggagatc tcttggctgc gcttcgcggc
     2041 gcgcgcgagc aacaacgccg cgatgttcgt cgaggcgatc aagagcagca gcgacaccgc
     2101 gccgaacagc aaccacaacg agccgcgaac cgcgccgacc gtcgtctcct tgagctgctc
     2161 gacatatacg ccggtgtcac gatcggtctc ggggtattgc tgcgtgagtc gcccttggac
     2221 cagctcgaga tccgccctcg cctgctcgat cgacacaccg ggttcgaggc ggccgtaacc
     2281 gcgcatccaa agattgcggc gattcagcac gtagggctcg taggcgagcg gcgtgaagat
     2341 gtccacgtcg cgatcaggaa agcggaacga cggcggcatc acaccgacga tcgggatcgc
     2401 ctgctcgccg atgcgtaccg tgcgctcgag cacgttcgga tcgctctcca gcttcgtcgt
     2461 ccagtagcga tgactgatca acgctacggg tgctgcgccg gcctggttgt ccgccggcgt
     2521 aaacgcccgg ccgatcagcg gcttcgtgcg ccagacgtcg agaaaacgcg gcgcgacgtg
     2581 cgcacgccgg tacttctccg gcaggtcgcc cgaggtttcg gacacgtcct cgcggtagta
     2641 acccgtgatc gcggcgaatg ccgtgctctg ctcgttccat tcctcgatgc gcacgggcgc
     2701 cgtgttgctg actggcgcgt tcggaatcga ctggttcacg acgacgagcc ggtcggcgtc
     2761 cgggaaaccg agcggctgca acaggatcgc gtcgatcgcc gagaacacgg cgctgttcgc
     2821 gcc
//
LOCUS       DATLEE010000366         4736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4883492, whole genome shotgun sequence.
ACCESSION   DATLEE010000366 DATLEE010000000
VERSION     DATLEE010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4883492"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1352)
                     /locus_tag="VM692_07880"
     CDS             complement(63..1352)
                     /locus_tag="VM692_07880"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HUQ52127.1"
                     /translation="MTKNATLTYENKSFELPTVSGTEAETAVDISQLRAKSGLITLDS
                     GYANTGACTSSITFIDGEQGILRYRGYPVEELAERSSFVETAYLLIFGELPTGTQLAD
                     FRRLLGQHEFIHEDMLHHFDGFPRNAPPMAILSAMINALGCFQPEVMQPEENEHFMQA
                     AARLMSKVRTIAAASYRKSIGRPVNYPRTELRYTANFLHMMFSLPHKLYQPDEVVVRA
                     LDQLLLLHADHEQNCSTSTVRMVGSSQANLHASVAAGVCALWGPLHGGANVAVIDMLT
                     ELKNSGEKLDAFVARVKDKDAHARLMGFGHRVYKNFDPRARIIKESCHALLKKLNVND
                     PLLETAMRLEEVALNDEYFIKRKLYPNVDYYSGIIMRALGIPTNMFTVMFAIGRLPGW
                     IAHWREVRIAGTQRIDRPRQIYTGATKRAYLPMEQRK"
     gene            1741..2838
                     /locus_tag="VM692_07885"
     CDS             1741..2838
                     /locus_tag="VM692_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HUQ52128.1"
                     /translation="MKTAEWNDWRWQNRNRIRSLEQLERIIAVTDEERAAILRHEGPL
                     PIGVTPYYMSLIDAADPSQGLRRTTIPTLTEFDRTLGEQDDPLGEDGHSPVPGLVHRY
                     PDRVLLLVTNFCSVYCRYCTRARLVGASGERALRKADIDRAIEYIAATPAVRDCLISG
                     GDPLSLDEDRLEYVLSRLRAIPHLEFVRIGSKQPVVQPMRVTPALTKMLRRYHPVWMS
                     LHFTHPDEVTPEVAEACGRLADAGIPLGSQTVLLKGVNDDVATLKKLFHALLKIRVRP
                     YYLYQCDPISGSSHFRTSVDKGLELISQLRGYTTGYAVPNYVVDAPGGGGKIAMLPDA
                     VIGRDGNDLLLRNFAGEQCRYPDPDGKLGQP"
     gene            2872..3855
                     /locus_tag="VM692_07890"
     CDS             2872..3855
                     /locus_tag="VM692_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HUQ52129.1"
                     /translation="MRIGLTYDLKDDYLALGLAEHEVAEFDSPATIAALESALQAAGH
                     DVERIGHVRSLAAKLVAGWRCDLVFNIAEGVAGFGREAQVPALLEAYGIPYTFSDPLV
                     SALTLHKGMAKHVARGCGVPTPGFVLVSTPAEATAVSLPLPLFAKPVAEGSSKGITAK
                     SLVRSRGALIDICTELLERYRQPVLVEEYLSGREFTVGILGTGGSARALATLEVVLLE
                     KADAGVYSYRNKTQWQGLVDYALLEPGALRSEVEAVALATWRCLGCRDAGRVDVRLDG
                     AGTAQMLEVNPLAGLTPGYSDLPLMAARCGMSYDALIAEILRCTAARLDAA"
     gene            3845..>4736
                     /locus_tag="VM692_07895"
     CDS             3845..>4736
                     /locus_tag="VM692_07895"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HUQ52130.1"
                     /translation="MRPKIALVYDAQAEIGRPDSSDTLVEARAISAALETLGYDTTRV
                     PVSLDLTALERTLRALAPQAVVNLVESLEGRGELVQVVPALLESLGLPFTGCSAWALG
                     ATQDKMAVKSLLAAADIATPATFGAAARSETNHGPWIVKAACEHSSLGIDDSSVVGSE
                     LVPQVLAKRRAEFGGRWFAERFVAGRELNAAVIAAPNGPRVLPIAEIRFDGFPADKPA
                     IVGYAAKWDADSFEYRNTNRSFAVEAELAARAERLALACWQLFALDGYARVDFRVDGS
                     GLLYVLEVNANPCLSPDAGFA"
BASE COUNT          733 a         1578 c         1603 g          822 t
ORIGIN      
        1 ccagtccgcc ggttttcgcc cacggggcga actccgcgca gaccatcagg attttcatcg
       61 cgctacttgc gctgctccat cggcaagtac gcgcgcttcg ttgcgccggt gtagatctgc
      121 cgcggccggt cgatgcgctg cgtcccggcg atccggacct cgcgccaatg cgcgatccag
      181 ccgggcaggc ggccgatcgc gaacatcacg gtgaacatgt tcgtcggaat gccgagcgcg
      241 cgcatgatga tgccgctgta gtagtcgacg ttcgggtaaa gcttccgctt gatgaagtac
      301 tcgtcgttga gcgcgacttc ctcgaggcgc atggcggtct cgagcagcgg gtcgttgacg
      361 ttgagcttct tcagcagggc gtggcacgac tctttgatga ttcgcgcacg gggatcgaag
      421 ttcttgtaga cgcggtggcc aaagcccatg aggcgcgcgt gggcgtcttt gtccttgacg
      481 cgggcgacga acgcgtcgag cttctcgccg ctgttcttga gctccgtcag catgtcgatg
      541 accgccacgt tagcgccgcc gtgcaggggg ccccagagcg cgcagacgcc ggccgcgacc
      601 gacgcgtgca gatttgcctg cgacgagccg accattcgga ccgtcgacgt cgagcagttc
      661 tgctcgtggt cggcgtgcag cagcaagagc tggtcgagcg cccgcacgac gacctcgtcg
      721 ggctgataga gcttgtgcgg cagcgagaac atcatgtgca ggaagttggc cgtgtagcgc
      781 agctcggttc gcgggtagtt gacgggccgg ccgatcgact tgcggtagct ggccgccgcg
      841 atcgtccgca ccttgctcat gagccgtgcc gcggcctgca tgaagtgctc gttctcctcg
      901 ggctgcatga cctccggctg gaagcagccg agggcgttga tcatggccga caggatcgcc
      961 atcggcggcg cattcctcgg gaagccgtcg aagtggtgca gcatgtcctc gtggatgaac
     1021 tcgtgctgac ccagcaggcg gcggaagtct gcgagctgag tccccgtcgg cagctcgccg
     1081 aagatcagga ggtaggccgt ctcgacgaag ctcgaccgct cggcaagctc ttcgacgggg
     1141 tagccgcggt atcgcagaat gccttgctcg ccgtcgatga acgtgatgct gctcgtgcaa
     1201 gcccccgtgt tcgcatagcc gctgtcgagc gtgatcagac ccgatttggc gcgcagctgc
     1261 gagatatcga cggccgtttc ggcttccgtg ccggagaccg tgggtaattc aaagctcttg
     1321 ttttcgtacg tgagcgtcgc gttcttcgtc atgagggagc ctccaaaacg tgggcgtatt
     1381 ctacgctcgg ggccacgcgg aagtgtgagc gctggggcgc agaacggcgt tcgcgtcgtt
     1441 cctgtttttg gagcgcgtcg ttacaaagaa agtgcttgca atctatgacc cgcgttttat
     1501 atttacgccc gttttcgttg caatgggctt cgagcccttg gaccaaaggt gagcaagctg
     1561 ctgtccctgg aggaagaacc tccgagtttg gcgcaagccg aacgcctcgt ccgaaagtcc
     1621 tctcaagctc tccgcgatct caaaagcagc gccgcaccgg tccagctagc gcgcgcggcc
     1681 ggcgcgcgtt tcgtcgtcag cccggccagt aacgaattcc gcaagcgtca cttccccggc
     1741 gtgaaaaccg ccgaatggaa cgactggcgt tggcagaacc gcaatcgcat ccgcagcctc
     1801 gagcagctcg agcgcatcat cgccgtcacc gacgaggagc gcgcggcgat ccttcggcac
     1861 gagggcccgc tgccgatcgg cgtcacgccg tactacatga gcttgatcga tgcggccgac
     1921 ccgtcgcagg gcttgcgccg cacgacgatc ccgacgctga ccgaattcga ccgcacctta
     1981 ggcgagcagg acgacccgct cggcgaggac ggccacagcc cggtgccggg cctcgtgcat
     2041 cgctacccgg atcgcgtgct gctgctcgtc acgaatttct gctccgtcta ctgccgctac
     2101 tgcacgcgag ccaggctcgt cggcgccagc ggcgagcgcg cgctgcgaaa ggccgacatt
     2161 gaccgcgcca tcgagtacat cgccgcgacg ccggccgtca gggactgtct gatctcgggc
     2221 ggcgacccgc tgtcgctcga cgaggaccgg ctcgagtacg tgctgtcgcg gctgcgcgcg
     2281 atcccgcatc tcgagttcgt ccgcatcggc agcaagcagc ccgtcgtgca gccgatgcgc
     2341 gtgacgcccg cgctgacgaa gatgctgcgg cgctaccatc cggtctggat gagcctgcac
     2401 ttcacgcacc ccgacgaggt gacgcccgag gtcgccgagg cgtgcggccg gctcgccgac
     2461 gccggcatcc cgctcggcag ccagaccgtg ctgctgaagg gcgtcaacga cgacgtcgcg
     2521 acgctgaaga agctgttcca cgcgctcttg aagatccgcg tgcggcccta ctatctctac
     2581 cagtgcgatc cgatcagcgg ctcgagccac ttccgcacgt ccgtcgacaa gggcctcgag
     2641 ctgatcagcc agctgcgcgg ctatacgacc gggtacgccg tgccgaacta cgtcgtcgac
     2701 gccccaggcg gcggcggcaa gatcgcgatg ctgccggacg cggtcatcgg ccgcgacggc
     2761 aacgacctgc tgctgcgcaa tttcgccggc gagcagtgcc gctatcccga cccggacggt
     2821 aagctcggcc aaccgtaatc aagcgctcct gcgctgaagc agagacacga cgtgcggatc
     2881 gggcttacct acgatctgaa agacgactat ttggcgctcg ggcttgccga gcacgaagtc
     2941 gcggagttcg attcgccggc cacgatcgct gcgctcgaaa gcgcgttgca ggccgcgggc
     3001 cacgacgtcg agcgcatcgg ccacgtgcgc tcactcgccg cgaagctcgt ggccggttgg
     3061 cgctgcgatc tcgtcttcaa catcgccgag ggggtcgccg gcttcggccg cgaggcgcag
     3121 gtgccggcgc tgctcgaggc ctacgggatc ccatacacgt tctccgatcc gctcgtctcc
     3181 gcgttgacgc tgcacaaggg catggcgaag cacgttgcgc gcggctgcgg cgtgccgacg
     3241 ccgggcttcg tgctcgtgag cacgccggcc gaggcaacgg ccgtgtcgtt gccgttgccg
     3301 ctgttcgcga agcccgtcgc cgaaggcagc agcaagggca tcacggcgaa gtcgctcgtg
     3361 aggtcgcgag gagcgctcat cgacatttgc accgagctgc tcgagcgcta tcggcagccc
     3421 gtgctcgtcg aggagtactt gagcgggcgc gagttcacgg tcggcatcct cgggaccggc
     3481 gggagcgctc gcgcgctcgc gacactcgag gtcgtgctgc tcgagaaggc cgacgccggt
     3541 gtctattcgt accgcaacaa gacgcagtgg caggggctcg tcgactacgc gctgctcgaa
     3601 cccggtgcgc tgcgctcgga ggtcgaggcc gtggcgctcg cgacgtggcg ctgtctcggt
     3661 tgccgcgacg ccgggcgtgt cgacgtgcgg ctcgacggcg cgggcacagc gcagatgctc
     3721 gaggtcaacc cgctggccgg gctgacgccg ggttactcgg acctgccgct gatggccgcg
     3781 cgctgcggca tgtcgtacga cgcgttgatc gccgagatcc tccgctgcac ggcggcgcgg
     3841 ctcgatgcgg cctaagatcg cgctggttta cgacgcgcag gccgagatag gccgccccga
     3901 ctcgagcgac accttggtcg aagcgcgggc gatctcggct gcgctcgaga cgctcggcta
     3961 cgacacgacg cgcgttcccg tctcgctcga cctgacggcg ctcgagcgca cgttgcgcgc
     4021 gctcgcgccg caggccgtcg tcaacctcgt cgagtcgctc gaggggcggg gggagctcgt
     4081 gcaagtcgtc cctgcgttgc tcgagtcgct cggcctgccg ttcacgggtt gctcggcttg
     4141 ggcgctcggc gcgacgcagg acaagatggc cgtcaagtcg ctgctggccg ccgccgacat
     4201 cgcgacgccg gcgacgttcg gcgccgccgc gcgctccgaa actaaccatg gcccgtggat
     4261 cgtgaaggcg gcctgcgagc actcgtcgct cggcatcgac gattcgtcgg tcgtcggcag
     4321 cgagctcgtg ccgcaggtgc tcgcgaagcg tcgcgcggaa ttcggcggcc gctggttcgc
     4381 cgagcggttc gtcgcgggcc gcgagctcaa cgcggccgtc atcgcggcgc cgaacggccc
     4441 gcgcgtgctg ccgatcgccg agatccgctt cgacggtttc cccgcggaca agcccgcgat
     4501 cgttggctat gcagcgaagt gggacgcgga cagcttcgag taccgcaaca cgaaccgctc
     4561 gttcgccgtg gaagccgagc tcgccgcgcg cgcggagcgg ctcgcactcg cgtgctggca
     4621 actgttcgcg ctcgacggct atgcgcgcgt cgactttcgt gtcgacggca gcggcctgct
     4681 ctacgtcctc gaggtcaacg ccaatccctg cctgtcgccc gatgccgggt tcgcgg
//
LOCUS       DATLEE010000367         4451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1162258, whole genome shotgun sequence.
ACCESSION   DATLEE010000367 DATLEE010000000
VERSION     DATLEE010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162258"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VM692_07900"
     CDS             <1..1386
                     /locus_tag="VM692_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUQ52131.1"
                     /translation="VLAFNLVRVFAARAPAGLPRVDEIAMSSEALAFTIVLAIASAAV
                     FGLAPALRLMRANPQDSLRGSGRATEAEPSRLRDWFVAAQIGLSAALLIVAGLLISSF
                     VRLGSVERGYDATNVLTAEVSLPPGAYANGEARRRFYDELVARLESQPGVAAAGVASV
                     LPLRGDAWADIVTVEGDERPLDQRPLMGYRTVSPDYLAALGIALYSGRSMQQNDFPRR
                     VAVVSRSAAESLWPGENPIGRQFRRGVPTEPAFEVVGVADDVRAAALDRAPPPLVYVA
                     LWERSPVAGAIAIRTALSSAVANALLRDTVRAIDAGIPVSNIATMTQIESDATAQRRF
                     QTMLVTTFAAAALLLAAIGIYGVVSYATARRTNEIGLRVALGAQPQDIRAMVLRQCLM
                     PIAIGLVAGVVAAFAAGRLISAMLFGTSAADPITFAAVVGIIVIVAAAASWLPARRAA
                     ALAPLTALRQE"
     gene            1412..2731
                     /locus_tag="VM692_07905"
     CDS             1412..2731
                     /locus_tag="VM692_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ52132.1"
                     /translation="MKQRRRLRFQTRVLVLVLLAVAPASIATVVLLYVTNATPGLRWT
                     IIGFLGVIVLIAIVAVRRHVVRPLQTLANMLEALREGDYSMRGRNVDPEDSFGEVMVE
                     VNTLSRTLYDQRLEALEAGALLQKIIADVDIAVFAFDAALRLRIVNRAGEALLGGTSE
                     RLQGRSAAELGLSGMLAEPSGRIVAHVFPGGGGRWEIRRRSFREGGKPHELLVISDLS
                     PALREEERQAWQRIVRVIGHELNSSLAPIKSMAGTVRKLVGRQPLPADWRDDAQAGLS
                     IIHDRAEALERFMGAYARLARLPPPTRRDAELAALVRRVASLHETRIAVESAARVHLS
                     IDPDQIEQVFINLMRNAVEASGEKGGVRVRWRGDGNAVLIEVEDDGPGLARTDNLWVP
                     FFTTKPHGTGIGLVLSREIVENHGGSIALANRVGAKGCIASVRLPLD"
     gene            complement(2771..3139)
                     /locus_tag="VM692_07910"
     CDS             complement(2771..3139)
                     /locus_tag="VM692_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HUQ52133.1"
                     /translation="MPFAKIQLKGQFAIRRDASTPLSLQIAHQLQAAIVEGRVSPGTR
                     LPSTRSLARLLGVSRNTVLAAYEELTARGFVRSRRGAGMYVASPVLGFDLKSVLRDAQ
                     YPARTLDTADPDGNAIRIVY"
     gene            3178..3597
                     /locus_tag="VM692_07915"
     CDS             3178..3597
                     /locus_tag="VM692_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ52134.1"
                     /translation="MITGGTATLYVSNMDNAIRFYTEVLGLKLTNRFGNHWATVRAGA
                     TLVIGLHPWSKKYPQPGTQGSVQIGLIVSRDEPIEAFAARLRRHGVEVSDVIRSEEAS
                     YVSFTDPDGNPIYVGDRDLVSDGAAEFNEELEDAALQ"
     gene            3692..>4451
                     /locus_tag="VM692_07920"
     CDS             3692..>4451
                     /locus_tag="VM692_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52135.1"
                     /translation="MPRETDAKKLERYETALAQFVERIMVDRYVLAVVAVGSLRLETI
                     WARHALSLWIIEADGVTRRIASDGTDERIFRILVENGINVHAEVIQRSRFKKMVEGAS
                     RTAFSHNFFAERRVVASKDASIDGWFKKANRMATKDQERELLAFSTWTIHSLIHARKR
                     LEVKGDLALASQETLGAAHSVAHTEIIRRGEICEGDVIYRAIEGAPELFKTIYLDVLA
                     KPKNKKVLTAALDAIGAYLEEHYEAHLKPLLDFLK"
BASE COUNT          742 a         1454 c         1497 g          758 t
ORIGIN      
        1 gtcctcgcat ttaatctcgt tcgagtcttt gcggcccgcg caccggctgg actgccgcgt
       61 gtcgacgaaa tcgcgatgag ctccgaggcg ttggcattca cgatcgtgct cgccatcgcg
      121 tcggccgccg tgttcgggct cgcgccggcc ttgcggctga tgcgagccaa cccgcaagac
      181 tcgttgcgcg gatccggtcg cgccacagaa gccgagccgt cgcggctccg cgattggttc
      241 gtagccgcgc agatcgggct cagcgccgcg ctcctgatcg tcgcggggct gctgatctcg
      301 agcttcgtgc ggcttggcag cgtcgagcgc ggttacgacg ccacgaacgt gttgacggcg
      361 gaggtcagct tgccgccagg cgcttatgcg aacggcgaag cgcggcggcg gttctatgac
      421 gagctcgtcg cgcggctcga gtcgcagcct ggagtcgccg cagccggcgt cgcctcggtg
      481 ttgccgctcc ggggcgacgc gtgggccgat atcgtgacgg tcgaaggcga cgaacgcccg
      541 ctcgaccagc ggccgctgat gggctatcga accgtctcgc ccgactatct cgcggcattg
      601 ggtatcgcgc tgtactcggg gcgctcgatg cagcaaaacg atttcccgcg tcgcgtcgcc
      661 gtcgtctcgc gcagcgcggc ggagagcctt tggccaggcg aaaacccgat cggccggcag
      721 ttccgccgcg gcgtcccgac ggagccggcg ttcgaggtcg tcggcgtcgc ggacgacgtg
      781 cgtgcggcag ctctcgatcg agcgccgccg ccgctcgtct acgtcgcgct ctgggagcgc
      841 tcgcccgtag ccggagccat cgcgatccgc acggcgctat cgtccgcggt cgctaacgcg
      901 ttactacgcg acaccgttcg tgcgatcgac gccggtatcc ctgtgtcgaa tattgcgacg
      961 atgacacaga tcgaaagcga cgctaccgcg caacgtcgtt ttcagacgat gctcgtgaca
     1021 acctttgccg ctgctgcctt gctgctcgcg gcgatcggaa tctacggcgt cgtctcgtac
     1081 gcgacagctc gccgcacgaa cgagatcggt ctgcgtgtcg cgctaggggc gcagccgcag
     1141 gacattcgcg ccatggtatt gcgccaatgc ctaatgccga tcgcaatcgg gctcgtggca
     1201 ggtgtcgtcg ccgcgttcgc ggccggccgg ctcatatcgg cgatgttgtt cggtacctcg
     1261 gcggcggatc cgatcacgtt cgccgcggtt gtcggcatca tcgtaatcgt ggccgccgcg
     1321 gcctcgtggc taccggcccg ccgtgcggcc gcgctcgcgc cgttgacggc tttgcggcaa
     1381 gaatgagcga gccgacgaag aagctccgcc cgtgaagcaa cgcaggcgcc tgcgtttcca
     1441 aacccgagtg ctggtcctcg tgctgctggc cgtcgcgccg gcctcgatcg cgacggtcgt
     1501 cttgctgtac gtgacgaacg cgacgcccgg cctgcgctgg acgatcatcg gtttcctggg
     1561 cgtcatcgtg ttgatcgcga tcgtggccgt gcggcgtcac gtcgtgcggc cgctgcaaac
     1621 gctcgccaac atgctcgaag cgctgcgcga gggcgactac tcgatgcgcg gccgcaacgt
     1681 cgaccccgag gactcgttcg gcgaggtcat ggtcgaggtc aacacgctga gccgcacgct
     1741 ctatgaccag cggctcgagg cgctcgaggc cggcgcgctg ttgcagaaga tcattgccga
     1801 cgtcgacatc gcggtgttcg cattcgatgc ggcgctgcgt ttgcgcatcg tcaatagagc
     1861 aggcgaggcg ctcctgggcg gaacgagcga gcgtctgcaa ggccgcagcg cggcggagct
     1921 cggcttgagc ggcatgctgg ccgagccgtc ggggcgcatc gtcgcgcacg tcttcccggg
     1981 cggcggcggc cgctgggaaa tccgccgccg cagtttccgc gaaggcggta agccgcacga
     2041 gctgctcgtc atctcggacc tgagccccgc gctgcgcgag gaagagcggc aagcgtggca
     2101 gcgcatcgtc cgcgtcatcg gccacgagct caacagctcg ctcgcgccga tcaagtcgat
     2161 ggccggtacc gtgcgcaagc tcgtcgggcg ccagccgctg ccggcggatt ggcgcgacga
     2221 cgcgcaggcc ggcttgtcga tcattcacga tcgcgccgag gcgctcgagc ggttcatggg
     2281 cgcctatgcg cggctcgcgc gtttgccgcc cccgacgcgg cgcgacgccg agcttgcggc
     2341 gctcgtgcgg cgcgttgcgt cgctgcacga gacgcgcatc gccgtcgagt ccgcagccag
     2401 agtgcatctg tcgatcgacc ccgatcagat cgagcaggtg ttcatcaacc tcatgcgcaa
     2461 cgccgtcgaa gcctcgggcg agaagggcgg cgtgcgcgtg cgctggcgcg gcgacggcaa
     2521 cgccgtgctc atcgaagtcg aggacgacgg gccgggcctc gcgcgcaccg acaatctttg
     2581 ggtgccgttc ttcacgacga agccgcacgg cacgggcatc ggcctcgtgc tgtcgcgcga
     2641 gatcgtcgag aaccatggcg gcagcatcgc gctggcgaac cgcgtcggcg ccaagggttg
     2701 catcgccagc gtgcgcttgc cgctcgactg atgcgggctg gagctaggcc cgattcaata
     2761 aacccccgat tcaataaaca atccgaatcg cgttcccgtc cgggtccgcc gtgtcgaggg
     2821 tgcgtgccgg gtactgtgca tcgcgcagga cgcttttcag gtcgaaacct aggaccggcg
     2881 aagccacgta cattccagca ccacggcggc ttcgaacgaa gcctcgggca gtcagttcct
     2941 cgtaggcagc gagcacagtg ttgcgcgaca caccgagcag acgcgcgagc gatcgtgtcg
     3001 atggcagccg agtacccggg ctcacgcggc cttctacgat cgccgcctgc agctggtgag
     3061 cgatctgcaa cgagagcggc gtcgacgcgt cgcgacgaat cgcaaactgg cccttcaact
     3121 ggatctttgc gaacggcacg gtatgggaga ctatcctccc gtaggaggac tgtcatcatg
     3181 atcaccggcg gaaccgcaac cctctacgtc tccaacatgg acaacgcaat ccggttctac
     3241 acggaggtgc tgggtctgaa actcaccaac cgcttcggca accattgggc gaccgtgcga
     3301 gccggggcga cactggtcat cggcctccat ccctggtcga agaagtatcc acagcccggg
     3361 acacagggat cggttcagat cggtctcatc gtttctcgcg acgagccgat cgaggcattc
     3421 gcggctcgtc tgcgtaggca tggcgtcgag gtcagcgacg tcatcagatc cgaagaagcc
     3481 agttatgtgt cgtttacgga ccccgacgga aacccgatct acgtcggcga tcgggatctt
     3541 gtcagcgatg gcgctgcgga gttcaacgag gaattggagg acgcggcgct ccagtgacgt
     3601 cgtgaaacgt tgccagggga ggggccccag cgcggcgccg tcgcgaaact gactacaatc
     3661 gtccctcggt tcacacgagg gaggcgattg catgccccgc gagactgacg ccaaaaaact
     3721 cgagcgctac gaaaccgcgc tcgcgcagtt cgtcgagcgc atcatggtag accgctacgt
     3781 gctggccgtc gtcgcggtcg gcagcttgcg gctcgagacg atctgggcgc gccacgcgct
     3841 gtcgctgtgg atcatcgagg ccgacggcgt gacgcggcgc attgcgagcg acggcacgga
     3901 cgagcgcatc ttccggatcc tcgtcgagaa cggcatcaac gttcacgccg aggtgattca
     3961 gcgcagccgc ttcaagaaga tggtcgaagg cgcgtcgcgg accgcgttct cgcacaactt
     4021 cttcgccgag cggcgcgtcg tcgcgagcaa ggatgcgtcg atcgacggct ggttcaagaa
     4081 agccaaccgc atggcgacga aggaccagga gcgcgagctg ctggccttct cgacgtggac
     4141 gatccattcg ctgatccatg cccgtaagcg actcgaggtc aaaggcgatc tcgcgcttgc
     4201 gtcgcaggaa acgttgggcg ccgcgcacag cgtcgcgcac acggagatca tccgccgcgg
     4261 cgagatctgt gaaggcgacg tcatctaccg cgcgatcgag ggtgcgccgg agctgttcaa
     4321 gacgatctac ctcgacgtgc tcgcgaaacc gaagaataag aaggtgctca ccgccgcgct
     4381 cgacgcgatc ggcgcgtacc tcgaggagca ctacgaggcg cacctgaagc cgctgctcga
     4441 tttcttgaag a
//
LOCUS       DATLEE010000368         3233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8405521, whole genome shotgun sequence.
ACCESSION   DATLEE010000368 DATLEE010000000
VERSION     DATLEE010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8405521"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VM692_07925"
     CDS             complement(<1..645)
                     /locus_tag="VM692_07925"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HUQ52136.1"
                     /translation="MVGGSRHAITLLASLSAIACAGEAAAQTIAATTQSSVADDAATT
                     KTLTIVRLAGVAKIDGLLDEPTWASATLVTDLHQIDPVEYAPPSQRSEIRLYYDDDAL
                     YVSARFWDTEADRITAQVLRQGEGMAGEDRFSVILDPYLDRRNGYRFQVNPNGVRWEA
                     LYQDTSSLESNWDGIWLGAATRDDDGWTAEMAIPFKTLSFNPNNREWGINFERTI"
     gene            complement(645..1028)
                     /locus_tag="VM692_07930"
     CDS             complement(645..1028)
                     /locus_tag="VM692_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HUQ52137.1"
                     /translation="MELVYGTILLALLEYIVMAALVGRARAKYGIHAPAMTGHPDFER
                     ANRVHVNTLENLMIFVPGVWIFAIYLSPRWAAGLGAVFVVARAVYAIGYLKAAEKRSI
                     GAGVTGCVNIVLVVGGLIGVARALF"
     gene            complement(1045..1896)
                     /locus_tag="VM692_07935"
     CDS             complement(1045..1896)
                     /locus_tag="VM692_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HUQ52138.1"
                     /translation="MSTPCIITVAITGSVPTKKDSPAVPITVPEQIESAHAAYEAGAA
                     LVHVHVRNDDGTTTSDPEKFGRFLAGIRKHCPDMIVQFSTGGRSGMGKERGGMLHLKP
                     EMASLTTGSVNFAKIIYENHPTLINELASSMLTHDIRPEIEIFDLAMLYNAAKLVAEG
                     LLKTPVHVQFVLGIPGALPADREVLEFELRQLTRMCPGATWTAAGIGRHQLTVNEWAL
                     ELGGHCRTGLEDNLKFDKVRFAASNAELVQRVADLVGKFDRSVATPAEARRILGLEPG
                     LMPRASR"
     gene            2036..2470
                     /locus_tag="VM692_07940"
     CDS             2036..2470
                     /locus_tag="VM692_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HUQ52139.1"
                     /translation="MPTLTLAQANQIVAKALEKARSMKIKPVTVVVLDEAGHLKAMQR
                     EDGASMFRYDVATGKAWAAVGMGASSRALAGRAKENPNFFITLAATAGGKFLPQIGGV
                     LIRDAGGAILGAAGASGGTGEEDEAVCAFGIEQAGLVADAKP"
     gene            complement(2473..3045)
                     /locus_tag="VM692_07945"
     CDS             complement(2473..3045)
                     /locus_tag="VM692_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52140.1"
                     /translation="MVGALAARLGKLTLALQALPSAAFAHRLDEYLQATLVAIEPGRI
                     ELSIRLTPGVDVADAVLEHIDFDRDGRISESESATYSELVRRDFELRVDRETLELATI
                     AAVLPTPAELRTGEGIIELELVAEAALAPGRHVLVLANRHDSRIGAYLFNASLPRSPT
                     IAIAQQRRNADQSIGEIDFTVAPSATAATP"
     gene            complement(3030..>3233)
                     /locus_tag="VM692_07950"
     CDS             complement(3030..>3233)
                     /locus_tag="VM692_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HUQ52141.1"
                     /translation="AAELEQGITDTWFAWSGPTERGAPAYFRIQGPTVHIEFAPQRGD
                     PAAHIHTIYRDPTNEYGARWSAR"
BASE COUNT          531 a         1083 c         1070 g          549 t
ORIGIN      
        1 gatcgtccgt tcgaagttga tgccccactc cctgttgttc ggattgaacg acagcgtctt
       61 gaacgggatc gccatctcgg ccgtccagcc gtcgtcgtcg cgcgtcgcag caccgagcca
      121 aatgccgtcc cagttgctct cgaggctcga cgtgtcttga tacaaggctt cccagcgcac
      181 gccgttcgga ttgacctgaa agcgataacc gttgcggcgg tcgagatacg gatcgaggat
      241 cacggagaac cgatcctcgc ccgccatgcc ttcgccctgg cgcaacacct gcgccgtgat
      301 gcggtccgct tccgtgtccc aaaaacgcgc cgatacgtac aacgcgtcgt cgtcgtaata
      361 gaggcgaatc tcggaacgct gcgacggcgg cgcgtactcg acggggtcga tctgatgcag
      421 atcggtcacg agcgtcgcgc tcgcccacgt cggctcgtcg agcaggccgt cgattttcgc
      481 gactccggcg agccgcacga tcgtcaacgt cttggtcgtg gcagcgtcgt cggcaacgct
      541 cgactgcgtc gtcgcggcta tcgtttgcgc ggccgcctcg cccgcgcagg cgatagcact
      601 cagcgacgcc agcagcgtga tcgcatggcg agagccgccg accactagaa caacgctcga
      661 gcgacgccga tcaatccgcc gacgacgagc acgatattga cgcatcccgt cacgccggcg
      721 ccgatgctgc gtttttccgc ggctttcagg taaccgatcg cgtacaccgc tcgcgcgacg
      781 acgaacacgg cgccgagccc ggcggcccac ctcgggctca agtagatcgc aaagatccag
      841 acgcccggca cgaaaatcat gaggttctcg agcgtgttga cgtgcacgcg gttcgcacgc
      901 tcgaagtccg gatggcccgt catcgcgggc gcgtggatac cgtacttcgc gcgcgcgcgg
      961 ccgacgagcg cggccatgac gatgtactcg agcaaggcca gcagaatcgt tccgtatacg
     1021 agctccatcg cccccccttt gttcttatcg gctcgctcgc ggcatcaggc cgggctcgag
     1081 accgaggatg cggcgcgcct cggccggggt cgcgaccgag cgatcgaact tgccgacgag
     1141 gtcggcgacg cgctgcacga gctccgcgtt gcttgcggcg aaccgcacct tatcgaattt
     1201 caagttgtcc tcgagccccg tgcggcaatg accgccgagc tcgagcgccc attcgttgac
     1261 cgtcagctga tggcggccga tgcctgccgc ggtccacgtc gcgccggggc acatacgcgt
     1321 aagctgacgg agctcgaact ctagcacttc gcgatcggcc ggcagcgcac cgggaatgcc
     1381 gagcacgaac tgcacgtgca cgggcgtttt cagtaacccc tcggcgacga gcttcgcggc
     1441 gttgtagagc attgcgagat cgaagatctc gatctccggc ctgatgtcgt gcgtcaacat
     1501 gctcgacgca agctcgttga tcagcgtcgg atggttctcg tagatgatct tggcgaagtt
     1561 gacggagccc gtcgtcagcg acgccatctc gggcttgagg tgcagcatgc cgccgcgttc
     1621 cttgcccatg ccgctgcggc cgcccgtcga gaactgcacg atcatgtcgg ggcagtgctt
     1681 acggatgccc gcgaggaagc ggccgaactt ctcgggatcg gaggtcgtcg taccgtcgtc
     1741 gttgcgcacg tgcacgtgga cgagcgcggc gccggcctcg taggccgcgt gcgccgactc
     1801 gatctgctcg ggaaccgtga tcggcaccgc cggactgtct ttcttcgtcg gcacggagcc
     1861 cgtgatggcg acggtgatga tgcacggcgt gctcatgatc tcaagtcctg tcagcggcgc
     1921 tctcgcggcc gccattctag acgcgcatcg tcaccatcac tacagcgcgg cgccttgcca
     1981 acggccaacg gcccgttatg ctgccagcct tgatgtttct tgacggagta cgcccatgcc
     2041 gaccctcacg ctcgcccaag ccaaccagat cgtggccaaa gccctcgaga aggctcgctc
     2101 gatgaaaatc aagcccgtga ccgtcgtcgt gctcgacgag gccggccact tgaaggcgat
     2161 gcagcgcgag gacggcgcga gcatgtttcg ctacgacgtc gcgaccggca aggcttgggc
     2221 agccgtcggc atgggcgcct cgagccgcgc attggccggc cgcgccaagg agaacccgaa
     2281 cttcttcatc acgctcgcgg caacggccgg cggcaagttc ctgccgcaga tcggcggcgt
     2341 gttgatccgt gacgcgggcg gcgccattct cggcgccgcg ggcgcgagcg gcggcacggg
     2401 cgaagaggac gaagccgtct gcgcgttcgg catcgagcag gcggggctcg tcgccgacgc
     2461 caagccctga cgtcagggcg tggcggccgt ggcgctcggc gccacggtga agtcgatctc
     2521 gccgatgctt tgatcggcgt tacgccgctg ctgcgcgatc gcgatcgtcg gcgaccgcgg
     2581 caacgaagcg ttgaacaggt aggcgccgat gcgcgagtcg tggcgattcg caagcacgag
     2641 cacgtgccgc cccggcgcga gcgccgcctc ggccacgagc tcgagctcga tgatgccttc
     2701 gcccgtgcgc agctcggccg gcgtcggcaa cacggcggcg atcgtcgcga gctccaaggt
     2761 ttcgcgatcg acccgcagct cgaaatcgcg acgcacgagc tccgagtacg tcgcgctttc
     2821 gctctcggag atccgcccgt ctcgatcgaa gtcgatgtgc tcgagcaccg cgtcggcgac
     2881 gtcgacgccc ggagtcaggc ggatcgacag ctcgatgcgg ccgggctcga tcgcgacgag
     2941 cgtcgcttgc aggtattcgt caaggcggtg ggcgaaggcc gccgacggca acgcctgcag
     3001 cgcgagcgtc agcttgccaa gccgggcggc tagcgcgccg accaccgtgc gccgtactcg
     3061 ttggtcgggt cgcgatagat cgtgtggatg tgcgcggccg ggtcgccgcg ttggggcgcg
     3121 aactcgatgt gcaccgtcgg accttggatg cggaaatagg cgggcgcgcc gcgctccgtg
     3181 ggaccgctcc aggcgaacca cgtgtcggtg attccttgct cgagctcggc cgc
//
LOCUS       DATLEE010000369         4851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1163345, whole genome shotgun sequence.
ACCESSION   DATLEE010000369 DATLEE010000000
VERSION     DATLEE010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163345"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1441)
                     /locus_tag="VM692_07955"
     CDS             complement(50..1441)
                     /locus_tag="VM692_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018139469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HUQ52142.1"
                     /translation="MTHVQLSTTVRRLPAGRALALLSALLASQIALGQPARELPTLAP
                     MIETISPAVVNIAVTSSLTERPGTQQDELLRRFFDFEGPPGRQREVEGAGSGVIVDAA
                     KGYILTNHHVIANADKITVTLAENRSLTARIVGSDEDSDLAVLQVEAANLKSIPFGDS
                     ATLRVGDYVIAIGNPFGFSNSVTSGIVSALGRSGLNSQAYEDFIQTDASINPGNSGGA
                     LVNLNGELVGINSAIISRTGGNVGIGFAIPVNMVRPVMDQLIATGTVRRGLLGVNIQD
                     VTPDLAATYGLAGNSGALVTRVTENSAAERANIQINDVIVSINGKRLRDSGSLRNFIG
                     LLQPGESVAVGLIRDGREQTLTAVLGANEPTAPVAAARPEKDKAELDSVFEGADLVDN
                     ATANGSPGLLVARVDPGSPAFERGLRPGDIITKVNRVRVRTLADAVPLMETARAIILE
                     VQRGTRSQLILMR"
     gene            1942..4089
                     /locus_tag="VM692_07960"
     CDS             1942..4089
                     /locus_tag="VM692_07960"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HUQ52143.1"
                     /translation="MNMAFELKATRLADIALQEASLNIWDTKYRLKTKAGEPVDRDID
                     GTYERVAKALAAVEEPARRGEWYDRFLWALQRGAIPAGRITSNAGAEYHKPATSTINC
                     TVSGTIRDSMHDILDKVHEAGLTLKAGCGIGYEFSTLRPRGAYVSGAGAYTSGPLSFM
                     DIYDKMCFTVSSAGGRRGAQMGTFDVGHPDAMDFVRAKREDGRLRQFNLSLLITDEFM
                     QAVRADQPWQLAFPVTRREAEEEKLDLDDEKQVVWREWPTKDNYVVDDRGLVACRVYR
                     TLPARRMWDVIMSSTYDFAEPGFVLIDRVNEMNNNWWCEDIRATNPCGEQPLPAYGAC
                     LLGSINLTKFVAAPFSDHAYFDWDEFREVVKVFTRMLDNVVDVNGLPLPQQRDEILRK
                     RRHGMGFLGLGSAITLLGMRYGSPESVKFTEDVSRELAVAGWQEGLELAKEKGAAPIL
                     LEEFDVTETMLRKRPEMVKDGWKVGQKIAGRILHTRYSRYMQRVRDVAPELVEQLEQH
                     GARFTHHSSIAPTGTISLSLANNASNGIEPSFAHHYFRNVIRQGRKTKEKVDVFSFEL
                     LAYRALVNPRALPGSTKPGEKLPDYFVSSEDITPKEHVDIQAASQKWIDSSISKTANV
                     PTDYPYDKFKDIYLYAHEKGLKGCTTFRFNPEAFQGVLVKEKDLKSTTYRFTLADGSI
                     VEAKGDEEIVYDGETHTAANLFDALKEGYYGKL"
     gene            4101..4799
                     /locus_tag="VM692_07965"
     CDS             4101..4799
                     /locus_tag="VM692_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HUQ52144.1"
                     /translation="MARITIDKKIVKYSVAKDEEPAKLAPEPKVDSESNVIRMHEKLE
                     RPEVLIGSTYKVKTPVSDHAMYVTINDIVLNEGTPHEQRRPFEIFINSKNLDHYQWIV
                     ALTRIMSAVFRKGGDVTFLVDELKAVFDPRGGYWQAGGKFMPSIIAELGYVIEKHLQT
                     IGLLRKPGLDVHQQKMVNEKRAEFEARAKQVDAFAKSSFPEGAQLCAKCSTAAVVMMD
                     GCMTCLNCGESKCG"
BASE COUNT          952 a         1458 c         1572 g          869 t
ORIGIN      
        1 tcgcctggga tgcgcgtgcg cgcacggcct cggccacgag cgccgttcgt tagcgcatca
       61 aaatgagctg gctgcgcgtg ccgcgctgaa cctcgaggat gatcgcgcgg gcggtctcca
      121 tgagcgggac cgcgtcggcc aacgtgcgca cgcgcacgcg attcaccttc gtaatgatgt
      181 cgccggggcg caagccgcgc tcgaaagccg ggctgcccgg atcgacgcgc gcgacgagca
      241 ggcccggtga cccgttcgca gtcgcgttgt cgacgaggtc cgcgccttcg aacacgctgt
      301 cgagctccgc cttgtccttt tcgggtcgcg ctgctgccac tggggcggtc ggctcgttcg
      361 cgccgagcac ggccgtcagg gtttgctcgc ggccgtcgcg aatcaagccg accgccacgc
      421 tctcgccggg ttgcagcagg ccgatgaagt tgcgcagcga gcccgaatcg cgcagccgct
      481 tgccgttgat gctgacgatg acgtcgttga tctgaatgtt tgcgcgctcg gcagccgagt
      541 tctcggtgac acgcgtgacc agcgcgccgg agttaccggc gagtccgtac gtcgctgcca
      601 gatccggcgt gacgtcttgg atgttcacgc ccaacaagcc gcgccgcacg gttcccgttg
      661 cgatcaactg atccatgacc gggcgaacca tgttcacggg aatcgcgaag ccgatgccga
      721 cgttgccgcc ggttcgcgaa atgatcgccg agttgatgcc aacgagctcg ccgttcaaat
      781 tcacgagcgc gccgcccgag ttgccggggt tgatcgatgc gtcggtctgg atgaagtcct
      841 cgtatgcctg gctgttcaag ccgctgcgtc cgagcgcgct gacgatcccc gacgtgacgc
      901 tgttggagaa gccgaacgga ttgccgatcg caatgacata gtcgccaacg cgcagcgtcg
      961 ccgaatcgcc gaaggggatg ctcttcaggt ttgcggcctc gacttgcagc acggcgagat
     1021 ccgagtcctc gtcggagccg acgatgcgcg ccgtgaggct gcggttctcg gcgagcgtga
     1081 ccgtgatctt gtcggcgttc gcgatgacgt gatggttcgt cagaatgtag cccttggcgg
     1141 catcgacgat gacgccggag cccgcgcctt ctacttcgcg ttgccggccg ggcggtccct
     1201 cgaagtcgaa gaaacggcgc agcaattcgt cctgctgtgt cccggggcgc tcggtcaggc
     1261 tgctcgtgac ggcgatgttc acgacggccg gcgaaatcgt ttcgatcatc ggcgccaagg
     1321 tcggcagctc acgcgcgggc tgtccgagcg cgatttggct ggccagtaac gccgacagca
     1381 aagccaaagc gcggcccgcg ggcaagcggc ggacggtggt tgatagctga acgtgagtca
     1441 tgcagggccc ctgattgaaa acgaaccgcg cgcgcggctc cgtcgacgca gcgttctaag
     1501 catagtgcgg cgccgcaatg atttcgacgt gaaccgccct gtgaattagt tgtggttagc
     1561 cgggggatag gctgggacta gcctgaacac aatatattgt ggttggcggc gcacgcttgg
     1621 tctcgctttc gatcgtgaaa atttccggat attgtccgga ggtgtacatc gcaacaatat
     1681 gtttctaata tgaaatcagg cgagatggat tttgtcataa aatcttgaca gccattctgc
     1741 gcgggcctag tctacgttcc aacgacacaa gatcttgtgt cgcgcctttt tcgaaaactc
     1801 acgacgggct ttaggctgcg cgagcagcca gcggggagtt tttcgcgcca aaaaaggccg
     1861 cgggaaattg aagccggcga atgaggaaag cgaagacggg ctgggggttt acggcgtacc
     1921 gttggggggg tcaagagcac tatgaacatg gcgtttgagc tcaaagccac gagactcgcg
     1981 gacattgctc tgcaggaagc ctcattaaat atctgggata cgaagtatcg cctgaagacg
     2041 aaggccggcg agccggtcga tcgagacatt gacggtactt acgagcgggt cgcgaaagcg
     2101 ctcgcggcgg tcgaggagcc tgctaggcgc ggcgagtggt acgaccggtt tctctgggcg
     2161 ctgcagcgcg gggcgattcc ggcgggacgc atcacctcga acgccggcgc cgagtatcac
     2221 aagccagcga cgtcgacgat caactgcacg gtatcgggca cgatccgtga ctcgatgcac
     2281 gacattctcg acaaggtgca tgaggccggc ctcacgctga aggccggctg cggcatcggc
     2341 tacgaattct cgacgttgcg gccgcgcggc gcatacgtca gcggcgccgg cgcgtatacg
     2401 tcgggccccc tgtcgttcat ggatatctac gacaagatgt gcttcacggt ctcgtcggcc
     2461 ggtgggcgcc gcggcgcgca aatgggcacg ttcgatgtcg gccatcccga cgcgatggat
     2521 ttcgttcgcg ccaaacgcga ggacggacgc ctccgccagt tcaacctgtc gttgttgatc
     2581 accgacgagt tcatgcaggc cgtgagggcc gaccagccgt ggcaactcgc gttccccgtg
     2641 acgcggcggg aggccgaaga agagaagctc gatctcgacg atgagaagca agtcgtctgg
     2701 cgcgagtggc cgacgaaaga caactacgtc gtcgacgacc gcggcctcgt cgcctgccgc
     2761 gtgtatcgga cgttgccggc gcggcgcatg tgggacgtca tcatgtcgtc gacctacgat
     2821 ttcgcggagc cgggcttcgt gctcatcgat cgtgtcaacg agatgaataa caactggtgg
     2881 tgcgaggaca tccgggcgac caatccgtgc ggcgagcagc cgctgcccgc gtacggcgcg
     2941 tgcctgctgg gctcgatcaa tctgacgaag ttcgtcgcgg cgccgttcag cgatcacgcc
     3001 tacttcgact gggacgagtt ccgcgaggtg gtcaaagtgt tcacgcgcat gctcgacaac
     3061 gtcgtcgacg tcaacggcct gccgctgccg cagcagcgcg acgagatcct gcgcaagcgt
     3121 cggcacggca tgggcttctt gggcttgggc tcggcgatca cgctgctcgg catgcggtac
     3181 ggatcgccgg agtccgtcaa gttcaccgag gacgtgtcgc gcgagctcgc cgttgccggc
     3241 tggcaagaag gcctcgagct cgcaaaagag aagggcgccg cgccgatcct gctcgaggaa
     3301 ttcgacgtca ctgaaacgat gctgcgcaag cgccccgaga tggtcaaaga cggctggaag
     3361 gtcggccaga aaatcgccgg ccgcatcttg cacacccgtt acagccgcta catgcagcgc
     3421 gtgcgcgacg tcgctccgga gctcgtcgag cagctcgagc agcacggcgc gcggttcacg
     3481 catcacagct cgattgcgcc gacggggacg atctcgttgt cgctcgcgaa caatgcgagc
     3541 aacggcatcg agccgtcgtt cgctcatcac tacttccgca acgtgattcg tcagggtagg
     3601 aagacgaaag aaaaggtcga cgttttctca ttcgagctgc tggcgtatcg cgcactcgtc
     3661 aacccgcgcg cactgccggg ttcgacaaag ccgggcgaga agttgccgga ctacttcgtg
     3721 agctccgagg acatcacgcc gaaggagcac gtcgatattc aagcggcgtc gcagaagtgg
     3781 atcgactcgt cgatctcgaa gactgcgaac gtcccgaccg attatccgta cgacaagttc
     3841 aaggacattt atctgtacgc gcacgagaaa ggcctgaagg gttgcacgac gttccgcttc
     3901 aatcccgaag ccttccaagg cgtcctcgtc aaagagaagg acctcaagag cacgacttac
     3961 cgcttcacgc tggccgacgg ctccatcgtc gaagccaaag gtgacgagga gatcgtctac
     4021 gacggcgaga cgcacacggc cgcaaatctg ttcgacgccc tcaaagaggg ctactacggc
     4081 aagctttgag agcacccaat atggcgcgaa tcaccatcga caagaaaatc gtcaagtact
     4141 ccgtcgcgaa ggacgaggag ccggcgaagc tggcacccga gccgaaggtc gacagcgagt
     4201 ccaacgtcat tcggatgcac gagaagctcg agcgtccgga ggtcctcatc ggctcgacgt
     4261 acaaggtcaa gacgcccgtg tcggaccacg cgatgtacgt gacgatcaac gacatcgtgt
     4321 tgaacgaagg cacgccgcac gagcagcggc ggccgttcga gatcttcatc aactcgaaga
     4381 acctcgatca ctatcaatgg atcgtcgcgc tgacgcgcat catgtcggcg gtattccgca
     4441 agggcggcga cgtgaccttc ctcgtcgacg agctcaaggc cgtgttcgat ccgcgcggcg
     4501 gctactggca ggccgggggc aagttcatgc cgtcgatcat cgccgagctc ggctacgtca
     4561 tcgagaagca cctgcagacg atcggcctgc tacgcaagcc aggcctcgat gtgcaccagc
     4621 agaagatggt gaacgagaag cgtgccgaat tcgaggcacg ggccaagcag gtggatgcct
     4681 ttgcaaagtc gtccttcccg gaaggcgcac agctctgtgc caagtgcagc accgccgccg
     4741 tcgtgatgat ggacggttgc atgacctgcc tgaactgcgg ggaatcgaag tgcggatgaa
     4801 tctatcgggc ggctgacacg ccctgacggt tggaaatgcg cgccgccgcc g
//
LOCUS       DATLEE010000370         2474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9356262, whole genome shotgun sequence.
ACCESSION   DATLEE010000370 DATLEE010000000
VERSION     DATLEE010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9356262"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VM692_07970"
     CDS             complement(<1..976)
                     /locus_tag="VM692_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HUQ52145.1"
                     /translation="MKLANVYYRGRNLVATELAPGELVGIASLPTRPQTGNTGVDMLD
                     IIRGGEPLRAALVASLDKIRNDDSVARIPTADVRWYPPVRNPGKICAVAMNNSSSNER
                     KISAPDHPAFFLKPPSCLVGHREPIRIRPYYGSVHPEPELAVVIGRVTRDIPAARAHD
                     AIYGYTIFNDITGNGMRAEDRFHYWAVYAKKDDPTQTERVEQHLSYAGRYKGTDTFGA
                     MGPWLVTKDEIANPDDLTVRCKVGGELIAEDSTRYYNYKVAEVVSFISQFHTLYPGDV
                     ISLGTAFKPGENRKSIHHANFQTVQGPVEVTIEGLGTQENPVVIEQKEL"
     gene            complement(997..>2474)
                     /locus_tag="VM692_07975"
     CDS             complement(997..>2474)
                     /locus_tag="VM692_07975"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HUQ52146.1"
                     /translation="HYIDGRVETGGARTAPVYNPATGAVLANVCLADAAVAARAVAAA
                     KAALPAWADTPPLKRARVLFRFKQLLDANFDELAALITREHGKVLADAKGELTRGLEI
                     VEFACGIPQLLKGQFSDNVGGDIDNWSLRQPVGVCVGITPFNFPVMVPMWMFPVAVAC
                     GNTFVLKPSERDPSTSLRLAQLFAEAGLPSGVLNVVHGDQESVGALLEHPDVAAVSFV
                     GSTPVAEHIYSTATKHGKRVQALGGAKNHMVVMPDADLEQAADALIGAGYGSAGERCM
                     AISVAVAVGHVADELVTKLTQRIRTLKVNDGSAPGADMGPLITAAHRAKVESYIEDGV
                     RAGAKLVVDGRGLRVAGRESGFFLGPSLFDCVEPAMRIYREEIFGPVLSIVRVPDFAS
                     AIELVNAHEYGNGVACYTSDGGVAREFARRIKAGMVGINVPIPVPLAFHSFGGWKRSL
                     FGDHHIYGEEGVRFYTRYKSVMQRWPSAGGKGPEFTMPTHD"
BASE COUNT          382 a          796 c          855 g          441 t
ORIGIN      
        1 cgagctcctt ctgctcgatg acgacggggt tctcctgcgt gccgaggcct tcgatcgtga
       61 cttcgacggg gccttgcacg gtttggaagt tcgcgtggtg aatcgacttg cggttctcgc
      121 cgggtttgaa cgccgtgccg agcgagatga cgtcgccggg gtacagcgtg tgaaactggc
      181 tgatgaagct gacgacctcg gccaccttgt agttgtagta gcgagtgctg tcctctgcga
      241 tcagctcgcc gccgactttg caccggaccg tgagatcgtc gggattcgcg atctcgtcct
      301 tcgtgacgag ccacggcccc atcgcgccga acgtgtcggt gcccttgtag cgccccgcgt
      361 aggagaggtg ctgctcgacg cgttccgttt gcgtcggatc gtcttttttc gcgtagacgg
      421 cccagtagtg gaaccgatct tcggcgcgca tgccgttgcc cgtgatgtcg ttgaagatcg
      481 tgtagccgta gatcgcatcg tgtgcacgcg ccgccggtat gtcgcgcgtc acgcggccga
      541 tgacgacggc aagctcgggc tccgggtgca cgctgccgta atacggacga atgcgaattg
      601 gctcccgatg accgacgagg cacgacggcg gcttcaaaaa aaacgcggga tgatcgggcg
      661 cgctgatctt gcgctcgttg ctcgagctgt tgttcatcgc cacggcgcag atttttccgg
      721 ggttgcgcac gggcgggtac cagcgaacgt cggccgtcgg aatgcgtgcg acggagtcgt
      781 cgttgcggat cttgtcgagc gaggcgacga gcgcggcccg cagcggctca ccgccgcgta
      841 tgatgtcgag catgtcgacg ccggtgttgc cggtctgtgg gcgcgtcggc aacgacgcaa
      901 tgccgacgag ctcgcccggt gcgagctccg tggcgacgag gttgcggccg cggtaataga
      961 cgttggcgag tttcatgcgc gcgatctcac cgcgtgtcag tcgtgcgtcg gcatcgtgaa
     1021 ctcggggcct ttgccgccgg cacttggcca gcgctgcatc acggacttgt agcgcgtgta
     1081 gaagcgcacg ccttcttcgc cgtaaatgtg atggtcgccg aataagctgc gcttccaacc
     1141 gccgaacgag tgaaacgcga gcggcacggg aatcgggacg ttgatgccga ccatgccggc
     1201 tttgatgcgg cgcgcgaact cgcgcgcgac gccgccgtcg ctcgtgtagc aagcgacgcc
     1261 gttgccgtac tcgtgcgcgt tgacgagctc gatggccgac gcgaagtccg gcacgcgcac
     1321 gatcgaaagc acgggcccaa agatctcttc gcgatagatg cgcatcgccg gctcgacgca
     1381 atcgaacagc gacggtccca ggaagaaacc gctttcgcgt cccgcgacgc gcaggccgcg
     1441 gccgtcgacg acgagcttgg cgccggcgcg cacgccgtcc tcgatgtaac tctcgacctt
     1501 cgcacgatgt gcggccgtga tcagcggccc catgtcggcg ccgggtgcgc tgccgtcgtt
     1561 gaccttcagt gtgcggatgc gctgcgtcag cttcgtgacg agctcgtcgg cgacgtggcc
     1621 gacggcgacg gccaccgaga tcgccatgca acgctcgccg gccgagccgt agccggcgcc
     1681 gatcagcgcg tcggcagcct gctcgaggtc cgcgtcgggc atgacgacca tgtggttctt
     1741 cgccccgccg agcgcttgca cgcgcttgcc gtgcttcgtc gccgtcgaat agatgtgctc
     1801 ggcgacaggc gtcgaaccga cgaagctaac ggccgcgacg tcgggatgct cgagcagcgc
     1861 accgacggac tcttggtcgc cgtgcacgac gttcagcacg ccgctcggca ggccggcttc
     1921 ggcgaacaac tgcgcaagcc tcagcgacgt gctcgggtcg cgctcgctcg gcttcaacac
     1981 gaacgtgttg ccgcaggcga cagcgaccgg gaacatccac atcggcacca tgaccgggaa
     2041 gttgaacggc gtgatgccga cgcagacgcc gacgggttgg cgcagcgacc agttgtcgat
     2101 gtcgccgccg acgttgtcac tgaactgccc cttgagcagc tgcgggatgc cgcacgcgaa
     2161 ctcgacgatc tcgagcccgc gcgtgagctc gcctttggcg tcggcgagca ccttgccgtg
     2221 ctcgcgcgtg atcagcgcgg ccagctcgtc gaaattcgcg tcgagtagct gcttgaagcg
     2281 gaacagcacg cgcgcgcgct tcagcggcgg cgtgtcggcc caggcgggca gggcggcctt
     2341 cgcggcggcg acggcgcgcg cggcgacggc cgcgtcggcg aggcaaacgt tcgcgagcac
     2401 cgcgcccgtc gctgggttgt agaccggcgc ggtgcgcgcg ccgcccgtct cgactctgcc
     2461 gtcgatgtag tggc
//
LOCUS       DATLEE010000371         2587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2653611, whole genome shotgun sequence.
ACCESSION   DATLEE010000371 DATLEE010000000
VERSION     DATLEE010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653611"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1814
                     /locus_tag="VM692_07980"
     CDS             270..1814
                     /locus_tag="VM692_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HUQ52147.1"
                     /translation="MADPLLVAKAGTIELCLLPALVNRHGLITGATGTGKTVSLQTIA
                     EQLSRIGVPVFMADVKGDLSGLAVAGGSNAKVTERAAVIGVELRYEACPVVFWDVFGK
                     RGHPIRATVSDLGPLLLARLLQLNDTQEGVLNLTFKIADDAGLLLLDVKDLRAMLQHV
                     GEHAAELTTEYGNVSAASVGAIQRGLLALESQGVEQLFGEPMLNIDDLLQTESGKGVI
                     NILAADRLMSSPKLYATLLLWMLSELFERLPEVGDPEKPRLVFFFDEAHLLFNDAPKA
                     LLEKIEQVVRLIRSKGVGVYFVTQNPLDIPDAVLGQLGNRLQHALRAFTPRDQRAVKS
                     AAETMRPNPKFAIEQAITELGVGEALVSLLDEKGRPGVTERALIVPPGSQLGPIEDAT
                     RARLVAASPVAGVYDKTIDRESAYERLKGRAEEPPPQERAPTWQRESAPAQRVPPQAQ
                     PQPQTSNDGGFMSDILFGRRGPRGGRQTQGVVEAMAKSAARSVGSQAGRALIRGVLGS
                     LLGGRR"
     gene            complement(1859..2230)
                     /gene="ssrA"
                     /locus_tag="VM692_07985"
     tmRNA           complement(1859..2230)
                     /gene="ssrA"
                     /locus_tag="VM692_07985"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2294..>2587)
                     /gene="smpB"
                     /locus_tag="VM692_07990"
     CDS             complement(2294..>2587)
                     /gene="smpB"
                     /locus_tag="VM692_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0070929 - trans-translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HUQ52148.1"
                     /translation="EAYLLGSHVTPLNTVSTHVVAEPKRTRKLLMNRREIDRLIGLVE
                     RKGYTLIALELYWSKNKVKVAVGLAKGKKQHDKRDTEKDRDWQRDKSRALKAR"
BASE COUNT          446 a          807 c          850 g          484 t
ORIGIN      
        1 gaaccacagc gcgtcgatgg tctcgccgcg attgaaggcg atggcaccca aggcaagcgt
       61 cgcgagcgcg acgactgcaa gccagccgag ccacgagacc ggcttcggca atcgttgcgt
      121 cattgtggtc gccattgcga cgtgtccccc gaaagtcttg tcgtgcgtgt agcgctgtaa
      181 gctaccgcag tccggggcgt gttgtatatt ccagtggctt gggtcgcgcg cgatcaaaag
      241 agcgcgaatt caagcgtcga ggttcatgaa tggccgatcc acttttggtt gcgaaggcgg
      301 gaacgatcga gctctgcttg ttgccggccc tcgtgaaccg ccacggcttg atcacgggcg
      361 cgacgggcac cggcaagacc gtctcgctgc aaacgatcgc cgagcaattg tcgagaatcg
      421 gcgtgccggt gttcatggcc gacgtcaaag gcgatttgtc aggcctcgct gtcgccgggg
      481 gcagcaacgc gaaagtcacc gagcgcgcgg ccgttatcgg cgtcgagctg cgctacgaag
      541 cgtgccccgt cgtgttttgg gatgtcttcg gcaagcgcgg ccacccgatc cgggcgaccg
      601 tctccgatct cggcccgctg ctgctcgcac gcctgctgca gttgaacgac acgcaggaag
      661 gtgtgctgaa cctgacgttc aagatcgccg acgacgcggg cttgctattg ctcgacgtca
      721 aggacttgcg cgcgatgctg caacacgtcg gcgagcacgc ggcggagctc acgacggagt
      781 acggcaacgt gtcggccgcg tcggtcggcg cgattcagcg cggtctgctc gcgctcgagt
      841 cgcagggcgt cgagcagctg ttcggcgagc cgatgctgaa catcgacgac ctcttgcaaa
      901 ccgagagcgg caagggcgtg atcaacattc tcgccgccga tcgattgatg agctcgccga
      961 agctgtacgc gacgctgttg ttgtggatgt tgtcggagct gttcgagcgg ctgcccgaag
     1021 tcggcgaccc cgagaagcct cgcttggtat ttttcttcga cgaggcgcac ctgctgttca
     1081 acgacgcgcc gaaggcgctg ctcgagaaga tcgagcaggt cgtccgcctc attcgatcga
     1141 agggcgtcgg cgtgtacttc gtgacgcaga atccgctcga cattcccgac gcggtgctcg
     1201 ggcagctggg taatcgactg cagcacgcgc tgcgcgcttt cacgccgcgc gaccaacgcg
     1261 ccgtgaagtc cgccgcggaa acgatgcggc cgaacccgaa gttcgcaatc gagcaagcca
     1321 tcaccgagct cggcgtgggc gaggctctcg tgtcgctgct cgacgagaag ggcaggcccg
     1381 gcgtgaccga gcgcgccttg atcgtgccgc ccggcagcca gctcggcccg atcgaagacg
     1441 cgacgcgtgc gcggctcgtc gctgcctcgc cggtcgccgg cgtctacgac aagacgatcg
     1501 accgcgagtc ggcgtacgaa agactgaagg ggcgcgccga agaaccgccg ccgcaggagc
     1561 gcgcacccac ctggcaacgc gagagcgcgc cggcgcagcg tgtgccgccg caagctcaac
     1621 cgcagccgca aacaagtaac gacggcggct tcatgtccga catcttgttc gggcggcgcg
     1681 gaccgcgtgg cggtcgtcaa acccaagggg tcgtcgaagc gatggcgaag agcgccgcgc
     1741 gctcggtcgg ctctcaggca ggccgtgcgc tgatccgcgg cgtgctcggc tcgctgctcg
     1801 gcggccgccg ctagttgaag gagtggcgtc cctctggccg gagcggccag agggacattg
     1861 gtggaggcgg cgggaatcga acccgcgtcc gtaagtcctc tgccatcagc tctacatgcg
     1921 tagtcgcgtc tattgtttta accggcagca acccgacgga cagggcacgc tgacggcgat
     1981 tccgtaaagg atcttagcga tctggtcccg gacttacctc gtcgcgatct tgtgatgatg
     2041 acgcctgaat gtccttgcgg acgctggacg cacaagcacg gctccagtca gacggcttaa
     2101 gccggttttt aggcggctag ggcgtagttg tcgtcgttgg caactaacag tttgcagact
     2161 gttttacgag gtatctgcac ctcggcatgc acctcaggtt tcgcgaccca cgtcgaagcc
     2221 agtcgccccc aaagtcacca cctagtttag ggctgccccc gggtgttgca agtgcgcccg
     2281 tcgcattcct agttcagcgc gccttcagcg cccgcgattt gtcgcgctgc caatcgcggt
     2341 ccttttcggt gtcgcgcttg tcgtgctgct tcttgccctt ggcgaggccg accgcgactt
     2401 tgaccttgtt tttgctccag tagagctcga gtgcgatcag cgtgtagccc ttgcgctcga
     2461 cgaggccgat cagtcgatcg atttcgcggc ggttcatcag caacttgcgc gtgcgcttgg
     2521 gctccgcgac gacgtgcgtc gacactgtat ttaatggagt gacgtgcgag ccgagcagat
     2581 acgcctc
//
LOCUS       DATLEE010000372         3268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1163614, whole genome shotgun sequence.
ACCESSION   DATLEE010000372 DATLEE010000000
VERSION     DATLEE010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163614"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VM692_07995"
     CDS             complement(<1..953)
                     /locus_tag="VM692_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HUQ52149.1"
                     /translation="MDFREEARRVAGYVGAALVAAVCVVPAAAQDAPARSAPPDPGQK
                     TVHIMRTATKPTIDGVIDEAVWATAARIDNLHQVNPVEYAEPSERTEIYLLYDDDALY
                     VAARLFEPPGELTANIMRQGASITQEDSLFVTLDPFNTRRGGYFFGLNAHGVRFDGLY
                     RNVSEYYTDWDSIWFAGALETEDGWTAEYEIPFKSLSFDPNSDTWGLNFSRSLETRNE
                     DMAWVSRNRRWDPSTAGLMTGLTGLDQGIGLDIVPSASVINRRVLRTDDPLTLAAEPD
                     LDTSDDDFEPSLDLFYKLTPQLNASLTFNTDFSATEVDDRQV"
     gene            complement(971..3223)
                     /locus_tag="VM692_08000"
     CDS             complement(971..3223)
                     /locus_tag="VM692_08000"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HUQ52150.1"
                     /translation="MRLKGWGFTAMLGAQLALAQAPAPLQDPGQHSVHIVRTTAAPPV
                     IDGKLDEAAWKSAALVDNLHQTAPIEYAIPDERTEIYLMYDDEALYIGAYLYDPEPDK
                     ITANNLRQADNVGQDDRFYVTIDPFMSLQRGYFFGLNPNGVRADGLYQNVTEFYGAWD
                     SIFYGAAGRFEGGWVAEMAIPFKSISFDPNTDTWGLNFSRGVVRKNENIAWVSRNRAY
                     NPAVSGLVTGFDRLDPHGLDIVPSASLRETRMASGGTSSDLEPSLDLVYKITPELNGS
                     LTFNTDFSATEVDDRQVNLTRFGLFFPEKRDFFLREADIFEFGRIGAQQDNISVGQPS
                     RENGRPFFSRRIGLNGSGQIVDLDYGAKVSGRVGNWDIGALSIRQDDYEIPNPLPVVG
                     AAPARIGAETLSVARGRTSVLGESTVGFIATSGDPRNDVDNSLVGADFQYRNTKLPGG
                     RSLEAEAWLQQSDTAGREGDDGAFGVGLRVPSSNRFRGGVAFKELEANYNPALGFVDR
                     VGVDVASFDVGYTHRPPRGSYVQSMFISMDGERAEQIGGRLQSQTMTLRPFLLTNRTG
                     DTLMMVYRDQWDNLPADFAISSLIPAIPRGDYRFDDLGMRLQMSNYRKVGGNLMYVSG
                     DFYGGTRVNINGDITWRPTSHFRTSVGYNYNDIELPQGSFETRLVRLGLDVVFSSTLS
                     LVNLVQYDNVSHTAGVNVRLHWIPEPGRELYFVINQNLDHALEPNLIDREFRTSQTDA
                     TVKMNYTFRF"
BASE COUNT          557 a         1040 c         1083 g          588 t
ORIGIN      
        1 acttgccggt cgtcgacctc ggtcgccgag aagtccgtgt tgaacgtcaa cgatgcattc
       61 aactgcggcg tcagcttgta gaacaggtcg agcgacggct cgaagtcgtc gtccgacgtg
      121 tcgagatcgg gctcggctgc caaggtcagc gggtcgtccg tgcgcagcac gcggcgattg
      181 atgacactcg cgctcggcac gatgtcgagg ccgatgcctt gatcgaggcc cgtgaggccc
      241 gtcatcagcc ccgcggtgct cggatcccag cgacggttcc gcgagaccca ggccatgtct
      301 tcgttgcgcg tctcgaggct gcgcgagaag ttgagacccc aggtgtcgct gttcggatcg
      361 aacgacaacg acttgaacgg gatttcatac tcggccgtcc aaccgtcttc ggtctcaagc
      421 gcgccggcga accagatgct gtcccagtcg gtgtagtact cggacacgtt gcggtacaag
      481 ccgtcgaagc gcacgccgtg ggcgttcaag ccgaagaagt agccgccgcg gcgcgtgttg
      541 aacgggtcga gcgtcacgaa cagcgagtct tcctgcgtga tcgaagcgcc ctgccgcatg
      601 atgttagccg tgagctcgcc gggcggctcg aacagcctcg cggcgacgta caacgcgtcg
      661 tcgtcgtaca gcaagtagat ctcggtgcgc tccgacggct ccgcgtactc gacggggttc
      721 acttggtgaa ggttgtcgat gcgcgccgcc gtcgcccaga cggcctcgtc gatcacgccg
      781 tcgatcgtcg gtttcgtcgc cgtgcgcatg atgtgcactg tcttctggcc cggatcgggc
      841 ggcgcgctcc tggccggcgc gtcttgggcc gcggccggaa cgacgcacac ggcggctacg
      901 agcgccgcgc cgacgtagcc ggctacgcgc cgtgcttctt ccctgaaatc cacgcagctt
      961 gctccgaacg ctagaaccgg aaggtgtagt tcatcttgac cgtcgcgtcc gtctgcgagg
     1021 tgcggaactc gcggtcgatc agattcggct cgagcgcatg gtcgaggttc tgattgatga
     1081 cgaaatagag ctcgcggccg ggctccggaa tccaatgcag ccgcacgttg acgccggccg
     1141 tgtgcgagac gttgtcgtac tgcacgagat tcacgagcga cagcgtcgag gagaacacga
     1201 cgtcgaggcc gagccggacg agccgcgtct cgaagctgcc ctgcggcagc tcgatgtcgt
     1261 tgtagttgta accgaccgac gtgcggaagt gcgacgtcgg ccgccacgtg atgtcgccgt
     1321 tgatgttgac acgcgtgccg ccgtagaaat cgccgctgac gtacatcagg ttgccgccga
     1381 ccttgcggta gttgctcatc tgcagccgca tgccgagatc gtcgaaacgg tagtcgccgc
     1441 gcgggatcgc gggaattagg ctgctgattg cgaaatccgc cggcaggttg tcccactggt
     1501 cgcggtaaac catcatcaac gtgtcgccgg tgcgattcgt cagcagaaac ggccgcaacg
     1561 tcatcgtctg cgactgcagc ctgccgccga tctgctcggc gcgctcgccg tccatcgata
     1621 tgaacatcga ctgcacgtac gagccgcgcg gcggccggtg cgtgtagccg acgtcgaacg
     1681 acgcgacgtc tacgccgacg cggtcgacga agccgagcgc cgggttgtag ttcgcctcga
     1741 gctctttgaa cgcgacgccg ccgcggaagc ggttcgagct cgggacgcgc aaaccgacgc
     1801 cgaacgcccc gtcgtcgcct tcacggccgg ccgtgtcgct ttgctgtagc caagcctcgg
     1861 cttccagcga gcggccgccc ggcagcttcg tgttgcgata ttggaagtcg gcaccgacga
     1921 gcgagttgtc gacgtcgttg cgcgggtcgc cgctcgtcgc gatgaacccg accgtcgact
     1981 cgccgagcac gcttgtccgg ccgcgcgcga cggacaacgt ctcggcgccg attcgcgccg
     2041 gcgccgcgcc caccacgggc aatggattcg gaatctcgta gtcgtcctga cgaatcgaga
     2101 gcgcgccgat gtcccagtta ccgacgcggc cgctgacttt ggcgccgtaa tcgaggtcga
     2161 cgatctggcc gctgccgttc aagccgatac gccgcgagaa aaacggccgg ccgttctcgc
     2221 gcgacggctg accgacggag atgttgtcct gctgtgcgcc gatgcggccg aactcgaaga
     2281 tgtccgcctc gcgcaagaag aagtcgcgct tctcggggaa gaacaagccg aagcgcgtga
     2341 ggttgacctg ccgatcgtcg acctcggttg ccgagaaatc cgtgttgaac gtcagcgagc
     2401 cgttgagctc cggcgtgatc ttgtagacga ggtcgagcga cggctcgagg tcgctgctgg
     2461 tgccgccgct cgccatacgc gtctcgcgca agctcgccga cggcacgatg tcgaggccgt
     2521 gcgggtcgag cctgtcgaag ccggtcacga ggcccgaaac cgcggggttg tacgcgcggt
     2581 tgcgcgagac ccaggcgatg ttctcgttct tgcgcacgac gccgcgcgag aaattcaggc
     2641 cccaggtgtc ggtgttcgga tcgaacgaga tcgacttgaa cggaatcgcc atctcggcga
     2701 cccagccgcc ctcgaagcgg ccggcggcgc cgtagaaaat gctgtcccat gcgccgtaga
     2761 actcggtgac attctggtac aggccgtcgg cgcgcacgcc gttcgggttc agcccgaaaa
     2821 aatagccgcg ctgcaggctc atgaacggat cgatcgtcac atagaaccga tcgtcctggc
     2881 cgacgttgtc ggcttgccgc agattgttgg ccgtgatctt gtcgggctcg ggatcgtaga
     2941 gataggcgcc gatgtagagc gcctcgtcgt cgtacatcaa atagatctcg gtacgctcgt
     3001 cgggaatggc gtactcgatc ggcgccgtct ggtgcaggtt gtcgacgagg gcggcgctct
     3061 tccacgcggc ctcgtcgagc ttgccgtcga tcacgggcgg cgccgccgtc gtgcgcacga
     3121 tgtgcacgga atgctggccg gggtcttgca gcggagccgg cgcctgagcg agcgccagct
     3181 gggcgcccag catcgccgtg aatccccacc ccttcaatcg catgatttct gatcgcccgc
     3241 tcaggaaagc gcgaacgata accgaaac
//
LOCUS       DATLEE010000373         2790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10645515, whole genome shotgun sequence.
ACCESSION   DATLEE010000373 DATLEE010000000
VERSION     DATLEE010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10645515"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VM692_08005"
     CDS             complement(<1..418)
                     /locus_tag="VM692_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52151.1"
                     /translation="MATSPDDQSRPSPQPSFSLSTLAAGIALAIGVGIGLLLNPRDAS
                     NPLEVAADAGTVARGTALFDVLEAAPSAEARELAAGVTATPVLTFGTGSGDYCRELEV
                     ASPRGATQLLACRRDGDWRLVLASYAPIAASDGVYRP"
     gene            complement(446..1357)
                     /locus_tag="VM692_08010"
     CDS             complement(446..1357)
                     /locus_tag="VM692_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HUQ52152.1"
                     /translation="MRVAVGAAFVALVVALPLPAVGQSGVHLNPVVAKLAAGETVYGL
                     INSGDLSFANARETTRAPVDFVYADMEHNPLDFPGLAAFLLGMTDKAAIQAKGNVQPN
                     VALFARFPPEADESGWVVKQALDIGLMGFIFNGVDTPAQALAAVRSMRYPPLRGARYP
                     EPKGVRGYGTAAAAFAWGVTAAEYERRADLWPLNPEGDLLAIVMIESAEGLGNVDEIA
                     AVPGVGALFLGAGADLSRSLGVTAASPELETAFQQILAACKRHDVACGLTAGNGEDVA
                     RRVREGWRIIRSTVPAITAGRALLEQR"
     gene            1460..2521
                     /locus_tag="VM692_08015"
     CDS             1460..2521
                     /locus_tag="VM692_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52153.1"
                     /translation="MSCKLVVGTSAFVAVTAAALGSPAPALAQAEASAEAHAAARIDP
                     NWAPPRTPWGHPDLQGIWTTDDMRGVPQQRADEFGTRQYVSDAEFAERASRREGAREI
                     QDRGAAGTFRNEEGTRTFGYTSMVIDPPDGKIPAVVAQASERRSTPGSFGLGPFNTMA
                     DFSLYDRCITRGVVGSFSPAVYGNGARIVQTPDTVAISYEMVHDTRVIPLDARPALSS
                     NFHLLMGDSRAHFEGNTLVIESANFTDRTAIGGVRHSEQLKLTERFTRIDPQMIDYEI
                     RVDDPLTWEKPWTMRMTITQQPGYEIYEYSCHEGNLAMRHALMGERAYERAAAEAAAK
                     GLPPPERVFERVNGPDRAR"
     gene            2550..>2790
                     /locus_tag="VM692_08020"
     CDS             2550..>2790
                     /locus_tag="VM692_08020"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52154.1"
                     /translation="MNLSSRIVSVCAALLCASLSSTAAAHHSFAAEFEMDKITEIEGR
                     VSAVHWVNPHIKIEVAATDGQSWEVEAGPVNLLPRM"
BASE COUNT          473 a          973 c          956 g          388 t
ORIGIN      
        1 cgggccggta cacgccgtcc gacgccgcga tcggcgcgta gctcgcgagc acgagccgcc
       61 aatcgccgtc gcggcggcac gcgagcagct gcgtggcgcc gcgcgggctc gcgacctcga
      121 gctcccggca gtagtcgccg ctgcccgtgc cgaacgtcag caccggcgtg gccgtcacgc
      181 cggccgcgag ctcgcgcgcc tcggcgctcg gcgcggcctc gagtacgtcg aacagcgccg
      241 tgccgcgcgc gacggtgcct gcatcggccg caacctcgag cggattcgaa gcgtcgcgcg
      301 gattcagcaa gaggccgatc ccgacaccga tcgccaacgc gatgccggcc gcgagcgtcg
      361 acagcgagaa cgacggctgc gggctcggcc ggctctggtc gtcgggactg gtggccatgc
      421 gcgcgagcct gagagccggg acgagctaac gctgctcgag cagcgcgcgg ccggccgtga
      481 tcgccggcac cgtcgagcgg atgatgcgcc agccttcccg aacgcggcgc gcgacatcct
      541 cgccgttgcc ggccgtgagc ccgcacgcga cgtcgtgccg cttgcacgcc gcgagtatct
      601 gctggaacgc cgtctcgagc tcgggcgacg cggccgtgac accgagcgac cgcgagagat
      661 cggcgccggc gccgaggaac agcgcgccga cgcccggcac ggccgcgatc tcgtcgacgt
      721 tgcccaagcc ttcggccgat tcgatcatca cgatcgcgag caagtcgcct tcggggttca
      781 gcggccacag atccgcgcgc cgttcgtact cggcagccgt gacaccccat gcaaacgcgg
      841 cggcagccgt gccgtagccg cgaacacctt tcggctcggg atagcgcgca ccgcgcagcg
      901 gcggatagcg catcgagcgc acggccgcga gcgcctgcgc cggcgtgtcg acgccgttaa
      961 agatgaagcc catcaagccg atgtcgagcg cctgcttcac gacccagccg gactcgtcgg
     1021 cctcgggagg gaagcgcgcg aacagcgcga cgttcggctg cacgttgccc ttcgcttgaa
     1081 tcgcggcttt gtcggtcatg ccgagcagga acgccgcgag ccccggaaag tcgagcgggt
     1141 tgtgctccat gtcggcatag acgaagtcga cgggggcgcg cgtggtctcg cgagcgttcg
     1201 cgaacgagag gtcgccggag ttgatgagcc cgtagacggt ttcgccggcc gcgagcttcg
     1261 cgacgacggg gttcaggtgc acgccgctct gcccaactgc gggcagcggc aacgccacga
     1321 cgagggccac gaacgccgcg ccaactgcga ctctcatggc gcgcgatcgt agcatgcgtg
     1381 cgtcccgcta ctcgaccgcc tcgactgcga cgcgccatac tcttccgcga ccggccggtc
     1441 gtgtttgagg gggtcgccga tgtcctgcaa gctcgttgtt ggcacaagcg cgttcgtcgc
     1501 cgtcacggcg gcggctctcg gctcgccggc accggcgctg gcacaagcgg aggcgtcggc
     1561 cgaggctcac gccgccgcgc gaatcgatcc gaactgggcg ccaccgcgca cgccgtgggg
     1621 gcaccccgat ctgcaaggaa tctggacgac agacgacatg cgaggcgtgc cgcagcagcg
     1681 ggccgacgag ttcggcacgc ggcaatacgt gagcgacgcc gagttcgcgg agcgagcgtc
     1741 acgccgcgaa ggcgcgcgcg aaattcagga ccgcggcgcc gcgggcacgt tccgcaacga
     1801 ggaaggcacg cgcacgttcg gctacacgtc gatggtcatc gacccgccgg acggcaagat
     1861 tcctgccgtc gtcgcgcagg cgagcgagcg gcgctcgacg ccgggctcgt tcggactcgg
     1921 gccgttcaac acgatggcgg atttcagcct gtacgaccgg tgcatcaccc gcggcgtggt
     1981 cggctcgttc tcgccggccg tctacggcaa cggcgcgcgc atcgtgcaaa cgcccgatac
     2041 cgtcgcgatc tcgtacgaga tggtccacga cacgcgagtc atcccgctcg acgcccgccc
     2101 cgcgttgagc agcaactttc acctgctcat gggcgactcg cgcgcgcact tcgaaggcaa
     2161 cacgctcgtc atcgagagcg cgaacttcac ggaccgaact gcaatcggcg gcgtgcgtca
     2221 cagcgagcag ctcaagctca cggaacgctt cacgcgcatc gatccgcaga tgatcgacta
     2281 cgagattcgc gtcgacgatc cgctgacgtg ggaaaagccc tggacgatgc gcatgacgat
     2341 cacgcagcaa ccgggctacg agatctacga gtactcgtgc cacgaaggca atctcgcgat
     2401 gcgccacgcg ctgatgggcg aacgcgccta cgagagagcg gcggccgaag cggccgccaa
     2461 agggctgccg ccgccggagc gcgtcttcga gcgcgtcaat gggcccgacc gcgcgcgtta
     2521 gcattagctt cagcaaaggt atacacaaca tgaatctttc atcgcgaatc gtatcggtct
     2581 gcgcagcgct gctgtgcgcg tcgttgtcgt ccacggccgc cgcgcaccac tcgttcgccg
     2641 cggagttcga gatggacaag atcacggaga tcgaaggccg cgtcagcgcg gtgcactggg
     2701 tcaatccgca catcaagatc gaggtcgcgg caaccgacgg ccagtcctgg gaagtcgagg
     2761 cggggcccgt caacttgcta ccgcgcatgg
//
LOCUS       DATLEE010000374         2353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_420526, whole genome shotgun sequence.
ACCESSION   DATLEE010000374 DATLEE010000000
VERSION     DATLEE010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420526"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..>2353)
                     /locus_tag="VM692_08025"
     CDS             complement(21..>2353)
                     /locus_tag="VM692_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HUQ52155.1"
                     /translation="GLIGGHVMITDGIGAGQIRAISNSAGTTLTVSSPFTVTPGSSSV
                     YVAVNSGGNVTAVAYDSGAWQEESTTAYAFSLFQKATTVMDMRQAVRLIPSTHNFFAA
                     DNQAFNGTGTAAAYGNIGYFSSGYSRKRQNAQDKLLPIDGTQPNPLVVASGTVDTATS
                     TQITDATATFGSYAPDPINFRYLNPGSQGQEYVLSIISGTGGKQSRRIASSTGTSVTV
                     EYPWGVTPAPGDFYEISEVVGMPEAVNPSEGYVANWNNKASNSDDGDNFGREHRVTFI
                     LERLAADNSWTRDDQRQLNKAVGGLDGRGVLGRQIVPRIRQAVTAEGNGGNMNVDTVL
                     AALEAHNAAPMFGRYFEDPVTATTGAGEVAFLNTFINNLATAIFGDEYAGAIGVPTGT
                     RALALVVHAIDSAANSPAGHYSQEYSGDYFNGTPWTQVVRDTFSALATGGIPAPSARG
                     TSSYNHPLAALNPALSFDPTPLGNRGTWEQIVESGPTVKGEFMFPLGQSGDINGTFTI
                     TPTLNITSIDSNVTSMQPIWRDWRFLPMLHVAETIEGGSSTGDADADGVLDGFEKWYF
                     DATTVAAKDNGDGDKSKLVDEFENGSDPTDADTDDDGLVDGFDGRDQDRLASGFTKMT
                     ASFKLNGIEDAFQMSGKFGTGSTVFDPAADAIDLRVETLNGDAVYAVTVPAGTLTGAD
                     GKYSYKDPDGAVNMLTSVQLKLGKAADKSASFKLKTAKQNLPTVATSGTPIYQVLVTI
                     HDASASSSRLLLDAREWDGDVAKTKAAKEPGTPVYH"
BASE COUNT          343 a          748 c          794 g          468 t
ORIGIN      
        1 aagtaaacgt ccccgctttt tcagtgatag accggggtgc cgggctcctt cgcggctttc
       61 gtcttggcga cgtcgccgtc ccactcgcgc gcgtcgagga gcaggcggct cgacgatgcg
      121 ctcgcgtcgt ggatcgtcac gagcacctgg tagatcggcg tgcccgacgt cgcgacggtc
      181 ggcaggttct gcttcgccgt cttcagcttg aaggacgccg acttgtcggc tgctttgccg
      241 agcttgagct gcacggacgt cagcatgttc acggcgccgt ccggatcctt gtaggaatac
      301 ttgccgtcag cgcccgtcag cgtgcccgcg ggcaccgtta ccgcgtagac cgcgtcgccg
      361 ttcagcgtct cgacgcggag atcgatcgcg tcggcggctg gatcgaacac cgtcgagccg
      421 gtgccgaact tgccgctcat ctggaaagca tcctcgatgc cgttcagctt gaacgacgcc
      481 gtcatcttcg tgaacccgct ggccaggcga tcctggtcac ggccgtcgaa gccgtcgacg
      541 agaccgtcgt cgtcggtgtc ggcgtcggtc ggatccgagc cgttctcgaa ctcatccacg
      601 agcttcgact tgtcgccgtc accgttgtcc ttcgcggcga ccgttgtcgc gtcgaagtac
      661 cacttctcga agccgtcgag cacgccgtcc gcgtccgcgt cgcccgtcga cgaaccgccc
      721 tcgatcgtct ctgcaacgtg cagcatcggc aggaagcgcc agtcgcgcca gattggctgc
      781 atgctcgtga cgttggagtc aatcgacgtg atgttgaggg tgggcgtgat cgtgaacgtg
      841 ccgttgatgt cgccgctctg gccgagcggg aacatgaact cgcccttgac ggtcggtccg
      901 ctctcgacga tctgctccca cgttccgcgg ttgccgagcg gagtggggtc gaacgacagt
      961 gccggattga gcgctgcgag cgggtgattg tagctgctgg tgccgcgcgc gctcggcgca
     1021 ggaatgccgc cggtcgcaag cgccgagaag gtgtcgcgca cgacctgggt ccatggcgtg
     1081 ccgttgaaat aatcgccgct gtactcctgc gagtagtgac cggccggcga gtttgccgcc
     1141 gagtcgattg catgcacgac aagcgcgagc gcacgcgttc ccgtgggcac accgattgcg
     1201 cccgcatact cgtcgccaaa gatcgccgtc gcgaggttgt tgatgaacgt gttgaggaag
     1261 gcgacttcgc cggcgcccgt cgtcgccgta acgggatctt cgaagtagcg gccgaacatc
     1321 ggcgcggcgt tgtgcgcctc gagggccgcg agcaccgtgt cgacgttcat gttacctccg
     1381 ttgccttcgg ccgtcacggc ctggcggatg cgcggcacga tctggcggcc gagcacgccg
     1441 cgtccgtcga ggcctccgac ggccttgttg agctgtctct ggtcgtcgcg cgtccacgag
     1501 ttgtcggctg cgaggcgttc gaggatgaac gtcacgcggt gctcgcgtcc gaagttgtcg
     1561 ccgtcgtccg agttgctcgc cttgttgttc cagttggcga cgtagccttc ggacgggttg
     1621 accgcctcgg gcattccgac gacctcggag atctcataga agtcgcccgg cgccggcgtg
     1681 acgccccacg ggtattcgac ggtgacgctg gttcccgtgc tcgaagcgat gcggcggctc
     1741 tgcttgccgc ccgtgccgct gatgatggac agcacgtact cctggccctg gctgcccgga
     1801 ttgagatagc ggaagttgat cgggtccggc gcgtagctgc caaaggtcgc ggtcgcgtcg
     1861 gtgatctgcg tcgatgttgc tgtgtcgacg gtgccgctcg cgacgacgag cgggttcggc
     1921 tgcgtaccgt cgatcggcag cagcttgtcc tgcgcgttct ggcgcttgcg cgaatagccc
     1981 gacgagaaat agccgatgtt gccgtacgcc gcggccgttc cggtgccgtt gaacgcctgg
     2041 ttgtcggcag cgaagaaatt gtgcgtgctc gggatcagcc gcacggcttg gcgcatgtcc
     2101 atgacggtcg tcgctttctg gaagagcgag aacgcgtagg ccgtcgtgga ttcttcctgc
     2161 cacgcgccgc tgtcgtaggc gacggcggtg acgttgccgc cactgttcac cgcgacgtac
     2221 accgagctcg agcccggcgt caccgtaaac ggcgacgaga ccgtcagcgt cgtaccggcg
     2281 gaatttgaga tcgcgcggat ctgaccggcg ccgatgccgt cggtgatcat cacatggccg
     2341 ccgatcaggc ccg
//
LOCUS       DATLEE010000375         2509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1166023, whole genome shotgun sequence.
ACCESSION   DATLEE010000375 DATLEE010000000
VERSION     DATLEE010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166023"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VM692_08030"
     CDS             <1..213
                     /locus_tag="VM692_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52156.1"
                     /translation="QVERVLSDDSGKTEEERIASREVTIMADKEIPYQLLKRVMATST
                     AADYGQLSLAVLQKASEARLATVAKN"
     gene            223..1197
                     /locus_tag="VM692_08035"
     CDS             223..1197
                     /locus_tag="VM692_08035"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUQ52157.1"
                     /translation="MSNAAVPLYRVYDLPWSTAFEDDARFKKLARGSLIGALVLAFLF
                     WALPQPKVDPATVEEVPKRLVRLVLERETPPPPPPVVREEPVPEPEPEPVVEQRVVEP
                     EPTPVPLPKPVVEPPPVDRAQQARDKARVAGLLPMAAQLQALRNNVTQLDRADNVGAG
                     ASDAPFAERSLVTSRVGTASGGINTAALSRNTGGSGIGGRETTQVENPVEAFAEAGGA
                     AQRSGTSDLASRSREEIERVFDANKGRIFTLYNRALRENPALQGKVVLRLTIAPDGRV
                     TACEIVSSELNDPELERGLVQRVLQFQFESRGDVESITTTKPIDFFPA"
     gene            1308..>2509
                     /locus_tag="VM692_08040"
     CDS             1308..>2509
                     /locus_tag="VM692_08040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ52158.1"
                     /translation="MTTTGVAAGQINLTEVMDNTRIGSLQIRVFALCLASLIMDGFDV
                     QAMGYVATEMFREWNVPQPALGGLLSLGNLGVLIGALVFSMVADKIGRRPVLVWATLF
                     FAVMTIATAYAQSIEQMRWLRLVAGIGLGCIIPNATALVGEFSPKRTRVAWVMCITMG
                     FTLGAAIGGFVANWLIPLWGWRSVFIFGGVVPLVISILMFLGLPESLQFLAVRRQKLE
                     QLSKWLKQLDPSLRVDASTQFKTNETSKAGVPFWHLFREGRAFVTTLLWIVNFTNILV
                     LYSLSGWLPTIFQNLMGYERSTAILLGTVVQIGGTVGAFGLAWMILRGGFTPMLALTF
                     AIATISVALIGQPGLSLPMLYVIVFVAGWCIVGGQPGLNALSASYYPTYLRSTGVGAG
                     LGVGRLGA"
BASE COUNT          409 a          809 c          829 g          462 t
ORIGIN      
        1 caggtcgagc gcgttctgag cgacgactcg ggcaagacag aagaagagcg tattgcgagc
       61 cgcgaagtca cgatcatggc cgacaaggaa attccgtatc agctgttgaa gcgcgtcatg
      121 gcgacgtcga cggctgccga ctacggtcag ttgtcgttgg ccgtgttgca gaaagcgtcc
      181 gaagcgcggc tcgcgacggt cgcgaagaac tgagggcgca gcatgtcgaa cgccgcagtt
      241 ccgctctatc gcgtctacga cctgccgtgg tccacggcgt tcgaggacga tgcgcgcttc
      301 aagaagctcg cgcgcggctc gctgatcggg gcgctggtgc tggcgtttct gttctgggct
      361 ttgccgcagc cgaaggtcga tccggcaacg gtcgaagagg tgccgaagcg gctcgtgcgg
      421 ctcgtgctcg agcgcgagac gccgccaccg ccgccgcccg tcgtgcgcga agagcccgtg
      481 ccggaaccgg agcccgagcc cgtcgtcgag cagcgcgtcg tcgagcccga gccgacgccg
      541 gtccctctac cgaagccggt cgtcgagccg ccgcccgtcg atcgagctca gcaagctcgc
      601 gataaagctc gcgtggccgg actactgccg atggccgcgc agcttcaggc gctgcgcaac
      661 aacgtcactc agctcgatcg tgccgacaat gtcggtgccg gagcgagcga cgcgccgttc
      721 gccgagcgct cgctcgtcac gtcgcgcgtc ggcacggcca gcggcggcat caacaccgcg
      781 gctttgagcc gcaacaccgg cggcagcggc atcggcggtc gcgagacgac gcaggtcgag
      841 aaccctgtcg aagctttcgc cgaggccggc ggcgccgcgc agcgcagcgg cacgagcgac
      901 ttggcgtcgc gcagccgcga agagatcgag cgcgtgttcg acgccaacaa aggtcgcatc
      961 ttcacgctct acaatcgagc gctgcgcgag aaccccgcat tgcaaggcaa ggtcgtgctg
     1021 cgtctgacga tcgcgccgga tggccgcgtc acggcctgcg agatcgtttc ttccgagctc
     1081 aacgaccccg agctcgagcg cggcctcgtg cagcgcgtgc tgcaattcca attcgaatca
     1141 cgtggcgacg tcgagtcgat cacgaccacg aagccgatcg acttcttccc ggcgtagaga
     1201 gcactctctg cccaccctcg cggtgggcgc gtgtatagtg agctggccag ggtgtcctag
     1261 cgctcacgtg cacttaggcc cccggaattc cagaggggaa tacaacaatg acgaccactg
     1321 gcgtcgcggc cgggcagatc aatctcaccg aggtcatgga caacactcgg atcggatcgc
     1381 tgcagattcg cgtcttcgcg ctgtgcctcg cttccttgat catggacggc ttcgacgtgc
     1441 aggccatggg ctacgtcgcg accgagatgt tcagggaatg gaacgtgccg cagcccgcgc
     1501 tgggcggcct cttgtcgctc ggcaatctcg gcgtactgat cggcgccttg gtcttcagca
     1561 tggtggccga caagatcggc cggcggcccg tgctcgtgtg ggcgacattg ttcttcgccg
     1621 tcatgacgat cgcgacggcc tacgcgcaga gcatcgagca gatgcgctgg ctgcggttgg
     1681 tcgccggcat cggcctgggc tgcatcatcc cgaacgcgac ggcgctcgtc ggcgagttca
     1741 gccccaagcg cacgcgcgtc gcctgggtca tgtgcatcac gatgggcttc acgctcggtg
     1801 ctgcgatcgg cggcttcgtt gcgaactggc tgatccccct ttggggctgg cgctcggtgt
     1861 tcatcttcgg cggcgtcgtg ccgctggtca tctcgatcct gatgttcttg ggcttacccg
     1921 agtcgctgca gtttctcgcg gtgcgacgcc agaagctcga gcagctctcg aagtggctca
     1981 agcagctcga tccgtcattg cgcgtcgatg cgagcacgca gttcaagacg aacgaaacga
     2041 gcaaggccgg cgtgccgttc tggcatttgt tccgcgaagg ccgagcgttc gtgacgacgc
     2101 tgttgtggat cgtcaacttc acgaacatcc tcgtgcttta ttcgctgtcg ggctggctgc
     2161 cgacgatctt ccaaaacctc atgggctatg aacgcagcac ggcgatcctg ctcggcaccg
     2221 tcgtgcagat cggcggtaca gtcggcgcgt tcggtcttgc atggatgatt ttgcgcggcg
     2281 gattcacgcc gatgctcgcg ctgacgtttg ccatcgcgac gatcagcgtc gcgctgatcg
     2341 gccaacccgg tctctcgttg ccgatgttgt acgtcatcgt gttcgtcgcc ggctggtgca
     2401 tcgtcggcgg ccaaccgggg ctcaacgcgc tgtcggcgag ctactacccg acctacttac
     2461 gttcgaccgg cgtcggcgcg ggactcggcg taggccggct cggcgcgat
//
LOCUS       DATLEE010000376         2229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9901783, whole genome shotgun sequence.
ACCESSION   DATLEE010000376 DATLEE010000000
VERSION     DATLEE010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9901783"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VM692_08045"
     CDS             <1..296
                     /locus_tag="VM692_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HUQ52159.1"
                     /translation="LTHTVRARSIMTIEGLEGPNGELHKVQQAMIDELGPQCGFCTPG
                     QIMSAVALLNENPTPSRDEVRHALSGNLCRCGAYDHYLNAVMRAANGERVSQA"
     gene            326..1327
                     /locus_tag="VM692_08050"
     CDS             326..1327
                     /locus_tag="VM692_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HUQ52160.1"
                     /translation="MSRDMMSAFELYQPDTVEGAVELLGRFGADGWALAGGNDSYDWF
                     KDRIKRPKQVVDLSGIAALKGIRETADGVEIGALTTLTEVERSSVIQNRYKLLADAAR
                     RVASPQIRNSGTIGGNVAQDARCWYYRYGVDCYRAGGNTCYADTPEGQNREHCLWGAD
                     RCVAVSPSDTAPALVALDATMVVLGPGGQREIKAEQFFIGPNIDIERMTVLEDGDVLT
                     AIRLPAEWSGARFYFEKVADRNTWDFPLVNIAAAMKVSGAAGAEKVDQIRIVAGAVQC
                     VPRRLTVVEEVVRGQAKTAETASLAGQSAVRGATPLNFNHFKIPLLQNLVTRAIRDA"
     gene            1362..1514
                     /locus_tag="VM692_08055"
     CDS             1362..1514
                     /locus_tag="VM692_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52161.1"
                     /translation="MELFRVSRDIYGREVLQGMSWDLLWVFFGLGVALILGHALYRLF
                     LAPKQQ"
     gene            1517..2179
                     /locus_tag="VM692_08060"
     CDS             1517..2179
                     /locus_tag="VM692_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HUQ52162.1"
                     /translation="MTTPSRATTGGSATGRVVRHALADRLFHWVGAACVLILLGTAFL
                     PIIGVEFGWVTIHWVTGLVLMAAVLFHIVRVLVRGTFGTMAVGRADVADAVDVTTSTL
                     ARKLPSRRPGKYSVAQKLIHHAFAVVVLTTLITGGMMLARIDTPWWQRNPYLLADATW
                     GIVYVLHGLAALLLITMVMMHIYFALRPEKLKFTRAMIRGWITRQEFDEHHDPKRWQV
                     DR"
BASE COUNT          380 a          694 c          766 g          389 t
ORIGIN      
        1 cgctgacgca cacggtgcgc gcgcgctcga tcatgacgat cgaagggctc gaagggccga
       61 acggcgagtt gcacaaagtg cagcaagcga tgatcgacga gctcggccca cagtgcggct
      121 tctgcacgcc ggggcaaatc atgtcggccg tcgcgttgct gaacgagaat ccgacgccgt
      181 cgcgcgacga ggtccggcac gcgttgtccg gcaacctgtg ccgctgcggc gcttacgatc
      241 attatttgaa tgcggtcatg cgcgcggcca acggcgagcg cgtgagccag gcgtaacgag
      301 cgctactaac taaggagctt tggctatgtc cagagacatg atgagcgcgt tcgagctgta
      361 tcagcccgac acggtggaag gagcggtcga gctcttgggg cgtttcggcg ccgacggttg
      421 ggcgcttgca ggcggcaacg acagctacga ctggttcaag gatcgcatca agcgaccgaa
      481 gcaggtcgtc gacttgagcg gcatcgcggc gctgaaaggc atccgcgaga ccgcggatgg
      541 cgtcgagatc ggcgcgttga cgacgctgac ggaagtcgag cgcagctcgg tcatccagaa
      601 tcgctacaag ctgctcgcgg acgccgcgcg ccgtgtcgcg agcccgcaga tcaggaactc
      661 cggcacgatc ggcggcaacg tggcgcagga cgcccgctgc tggtactacc gctacggcgt
      721 cgattgctat cgcgcgggcg gcaacacctg ctatgccgat acgcccgaag gccagaaccg
      781 cgagcattgc ctctggggtg ccgaccgctg cgtggccgtg agcccgtcgg atacggcgcc
      841 ggcgctcgtc gcgctcgacg cgacgatggt cgttttgggt cccggcggtc agcgcgaaat
      901 caaagccgaa cagttcttca tcggtccgaa catcgacatc gagcgcatga cggtgctcga
      961 ggacggcgac gtgctgacgg cgattcggct gccggccgag tggagcgggg cgcggttcta
     1021 cttcgagaag gtcgcggatc gcaatacgtg ggacttcccg ctcgtcaaca tcgcggcggc
     1081 catgaaggtc agcggcgcgg cgggcgccga gaaggttgac cagattcgca tcgtggccgg
     1141 cgccgtgcag tgcgtgccgc ggcggctcac ggtcgtcgaa gaggtcgtgc gcgggcaggc
     1201 gaagacggcc gagacggcgt cgctcgcagg ccagagcgcc gtgcgcggcg cgacgccgct
     1261 taacttcaac cacttcaaga ttccgctgct gcagaacctc gtcacgcgcg ccattcggga
     1321 cgcctaggcg ccgcgcctgt aggctcgagt agcgagcacg cgtggagttg ttccgagtca
     1381 gccgcgacat ctacggccgc gaagtgctcc agggcatgtc gtgggacttg ctgtgggtgt
     1441 tcttcggctt aggagtcgcc ctgatcttgg ggcacgcgct gtaccggttg tttctcgcgc
     1501 ctaagcaaca atagtcgtga ccacgccttc acgcgccacc actggcggtt cggccaccgg
     1561 ccgcgtcgtc cggcacgccc tcgccgaccg cctgttccac tgggtcggcg cggcctgcgt
     1621 gctgatcctg ctcggcacgg cgttcctgcc gatcatcggc gtcgaattcg gttgggtgac
     1681 gatccattgg gtgacgggcc tcgtgctgat ggccgcggtg ctgttccaca tcgtgcgcgt
     1741 gctcgtgcgc ggcacgttcg gcacgatggc ggtcggccgc gccgatgtcg ccgacgccgt
     1801 cgatgtcacg acgtcgacgc ttgcgcgcaa gctgccgagc cgtcggcccg ggaagtattc
     1861 tgtcgcgcag aagctcatcc atcacgcgtt tgccgtcgtt gtgctcacga cgttgatcac
     1921 gggcggcatg atgctcgcgc gcatcgacac gccgtggtgg cagcgcaatc cctatttgtt
     1981 ggccgatgcg acgtggggca tcgtctacgt gctgcacggg ctcgctgcgt tgctgctgat
     2041 cacgatggtc atgatgcaca tctatttcgc gctgcggccc gaaaagctga agttcacgcg
     2101 agcgatgatc cgcggctgga tcacgcgcca agagttcgac gagcaccacg atcccaagcg
     2161 ctggcaggta gaccgatgac gacgagcgac gacttgaagc aacgcatcga ggcgatcgaa
     2221 acgggttac
//
LOCUS       DATLEE010000377         3379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4128972, whole genome shotgun sequence.
ACCESSION   DATLEE010000377 DATLEE010000000
VERSION     DATLEE010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4128972"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3361)
                     /locus_tag="VM692_08065"
     CDS             complement(<1..3361)
                     /locus_tag="VM692_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase PknK"
                     /protein_id="HUQ52163.1"
                     /translation="MTLEAAPFPGYQHGQPLHDSSASSVYRARRASDGACVVIKRSQG
                     HPASARQLTRYRNEFELLRSLDCSGVVKAYDLLRHDGQLALVLEDLPGVSLRRWIESA
                     GNAPLRERLELAIRLADIMAAVHGANIIHKDISSHNVVYDADTRRCTLIDFGIATRLR
                     SEESKFQAAAALEGTLAYIAPEQTGRMNRSLDYRADLYSIGVTLYELFTGALPHVSAD
                     PLEMVHFHIAGKPVPPCDRDARVPEALSDIVLKLLQKEPEDRYQSASGLAADLRQCQA
                     QLDAAGKIERFPLGSADAIDRFDPPQKLYGRGAETKLLLSSFERVARGGVETIAVAGQ
                     PGIGKTSLVQEIYQPITRQRGYFVAGKFDLLQQNVPFSALVTALQDLVQQLLTEGEDE
                     IAAWREAIESAIYPNGQLIVDVVPALGLIIGPQPRVPELEALEAQNRFNLAFQNFVQV
                     FCKKSHPLVLFLDDMQWADAASLNLITLIVSARATESLLLVLTYRDSEVAANHPFMLA
                     IKEQAKQGVPVQTIELAPLGVPEVAEFVADTLREDRAAATPLAEIIQQKTAGNPFFMR
                     QFLQALYGAQLIRFDSQAKRFRYDTAAVKDAAITENVAELLATKLQRLPESTQRTLRV
                     AAVIGGRFELRLLAKVLQQSAAATDESLRPAIEAGLIAPLAGLESLDADAFQSPLVYG
                     RFAFRHDRVQQAAYVTLPAAERPGLHLAIGRAWLAMTPAAELESRLFDIVGHLNAGRA
                     LLDDDAERRRLADLNRRVGIKARDSTAYELAVTSFRAAIELDGAAAWRERHAVHFDTH
                     RRLAEALGLTADAAGALQVVEQSLGHAASLIDRTHLNSIKTNVLLIMGRIPEALACGR
                     AAAREFGVDLPEQPEQVRALLQREIQTIRDRAAAVGIEKLLDLPAMSDPGQMALIGLL
                     VHCMPAAFQTDQDSYALLCCTMVRLSLEHGNCALSARAYGSFAALVSSALREYGDAYR
                     FAKLGVDLAHKLNETSTLAGVYFLFAMFASHWIKPIDESIDLYQQAVRFGLQSGDHVH
                     AGYSVARRFSHLQVRGMPLAELRQEGNAALEMLHRISDAANCDFLEPRLGLIDWLRGD
                     RRYGNTLGNAARDEAAQTAAIQARGNRSFEAD"
BASE COUNT          564 a         1113 c         1136 g          566 t
ORIGIN      
        1 agtcggcttc gaacgagcga ttgccgcgcg cctggattgc ggccgtttgc gcggcttcgt
       61 cgcgcgcggc attgcccagc gtattgccgt agcgccgatc gccgcgcagc cagtcgatga
      121 gaccgaggcg cggctcgagg aagtcgcagt tcgccgcgtc actgatccga tgcagcatct
      181 cgagggcggc gttgccttct tgccgcagct ccgcaagcgg catgccgcgc acttgcagat
      241 gcgagaagcg gcgcgcgacg ctgtagcccg catgaacgtg atcgccgctc tgcaagccga
      301 aacggacggc ttgctggtag aggtcgatgc tttcgtcgat gggcttgatc caatgcgacg
      361 cgaacattgc gaacaggaag tagacgccgg cgagggtcga cgtctcgttg agcttgtgcg
      421 ccaggtcgac gccgagcttg gcgaaccgat aggcgtcgcc gtactcgcgc agcgcgctgc
      481 tgacgagcgc cgcgaacgag ccgtaggcgc gtgccgacag agcgcagttg ccgtgctcga
      541 gcgacagtcg gaccatcgtg cagcaaagca acgcgtagct gtcctgatcg gtctggaacg
      601 ccgccggcat gcagtgcacg agcaagccaa tcagcgccat ctggccggga tcgctcatcg
      661 ccggcaggtc gagcagtttt tcgataccga cggctgccgc gcggtcgcga atcgtctgga
      721 tctcgcgctg cagcagcgcc ctgacctgct ccggctgctc cggcaaatcg acgccgaact
      781 cgcgcgcggc cgcgcgaccg cacgccagcg cctccggaat gcgccccatg atcagcagca
      841 cgttcgtctt gatcgagttc aagtgcgtgc ggtcgatcag cgaagcggca tggcccagcg
      901 attgctcgac gacctgcagg gcccccgctg catcggccgt gagccccaac gcctcggcca
      961 ggcggcgatg cgtgtcgaag tgcacggcgt ggcgctcgcg ccaggctgcg gcgccgtcga
     1021 gctcgatggc cgcccggaag ctcgtgacgg cgagctcgta cgccgtcgag tcgcgcgctt
     1081 tgatgccgac gcgtcgattg aggtcggcga ggcgacgccg ttcggcgtcg tcgtcgagca
     1141 acgcgcggcc agcgttcaag tggccgacga tgtcgaacaa gcgactctcg agctccgcgg
     1201 ccggcgtcat ggcaagccag gcgcgaccga tcgctagatg caggcccggc cgctcagccg
     1261 ccggtagggt cacgtaggcc gcctgctgca cgcggtcgtg gcggaacgcg aaccgcccgt
     1321 agacgagcgg cgactgaaac gcgtcggcgt cgaggctctc gaggccagcc agcggcgcga
     1381 tgagaccggc ttcgatggcc ggtcgcaggc tttcgtccgt cgccgccgcc gattgctgca
     1441 gcactttcgc gagcagacgc agctcgaagc ggccgccgat gacggccgcc acgcgcaacg
     1501 ttcgttgcgt gctctcgggc aagcgctgca gcttcgtggc gagcagctcg gccacgttct
     1561 cggtgatcgc cgcgtccttg acggccgcgg tgtcgtagcg aaaccgcttc gcttgactgt
     1621 cgaagcggat cagctgcgcg ccgtacagcg cctggaggaa ctggcgcatg aagaacggat
     1681 tgccggccgt cttctgctgg atgatctcgg ccagcggcgt cgcggccgcg cggtcctccc
     1741 gcagcgtgtc ggcgacgaac tcggcgacct cggggacacc gagcggcgcg agctcgatcg
     1801 tctgcacagg cacgccctgc ttcgcttgct cttttatggc caacatgaac gggtgattgg
     1861 ccgcaacctc ggagtcgcga taggtcaaga cgaggagcag cgactccgtc gcccgcgccg
     1921 agacgatcag cgtgatcaag ttcaggctcg ccgcatcggc ccactgcatg tcgtccagga
     1981 acaggacgag cggatgcgac ttcttgcaga acacctgcac gaaattttgg aacgccaggt
     2041 tgaagcggtt ctgcgcttcg agcgcctcga gctccggcac gcgcggttgg ggaccgatga
     2101 tgaggccgag cgccggcacg acgtcgacga tcagctggcc gttcggataa atggccgact
     2161 cgatggcttc gcgccaggcc gcgatttcgt cctcgccctc ggtcaacagc tgctgcacga
     2221 gatcctgcag cgccgtcacg agggcgctga acggcacgtt ttgctgcaac aaatcgaact
     2281 tgccggcgac gaagtagccg cgttgccgcg tgatcggctg atagatctct tgcacgagcg
     2341 acgtcttgcc gatgccgggc tggcccgcca cggcgatcgt ttcgacgccg ccgcgcgcca
     2401 cgcgctcgaa gctcgatagg agcagcttcg tctccgcgcc gcggccatac agcttctgcg
     2461 gcggatcgaa gcggtcgatc gcatccgccg agcccagcgg gaagcgctcg atctttcccg
     2521 cggcgtcgag ctgcgcttgg cactggcgca gatccgccgc gaggccgctc gcgctttgat
     2581 aacggtcctc gggctccttc tgcaacagct tcagaacgat gtccgacaac gcttccggca
     2641 cgcgcgcatc gcggtcgcag ggcggcacgg gtttgcccgc gatatggaaa tgaaccatct
     2701 cgagcggatc cgcactgacg tgcggcaacg cgcccgtgaa cagctcgtac agcgtgacgc
     2761 cgatcgaata gagatcggcg cggtagtcga ggctgcggtt catgcgtccc gtttgctcgg
     2821 gcgctatgta agcgagagtg ccctcgagcg cagcggccgc ctgaaacttg ctctcctcgc
     2881 tgcgcagtcg cgtcgcgata ccgaagtcga tcagcgtgca gcgtcgcgtg tcagcgtcgt
     2941 agacgacgtt gtggctgctg atgtccttgt gaatgatgtt ggcgccgtgc acggccgcca
     3001 tgatgtcggc cagccgaatg gcgagctcga gccgctcgcg caacggcgcg ttgccggcgg
     3061 actcgatcca acgccgcagc gacactccgg gcaagtcctc gagcacgagc gcgagttggc
     3121 cgtcgtgacg caacagatcg taggctttga cgacgccgct gcaatcaagc gagcgcaaca
     3181 gctcaaactc gttgcgatag cgcgtgagct ggcgcgccga tgccgggtgg ccctgcgaac
     3241 gtttgatgac gacgcacgcg ccatcgctcg cacgacgcgc gcgatatacg gacgacgcgc
     3301 tgctatcgtg aagcggctgc ccgtgctggt agcccgggaa cggtgctgcc tccaacgtca
     3361 tacccctaag ctctatctc
//
LOCUS       DATLEE010000378         4207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8409510, whole genome shotgun sequence.
ACCESSION   DATLEE010000378 DATLEE010000000
VERSION     DATLEE010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8409510"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1976)
                     /locus_tag="VM692_08070"
     CDS             complement(<1..1976)
                     /locus_tag="VM692_08070"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HUQ52164.1"
                     /translation="MPRGFDLLKRLLAAVAVLVLLLAAIAYTVVAASLPRHSGRAQLV
                     GLEAPLTIALDARAIGTVRGESFLDALRGQGYLHAQERFFQMDLLRRASAGELAELFG
                     ERALANDRSQRAFGFSELARRIRASLPAEQNEWLQAYTQGVNAGLADLGARPPEYWLA
                     GSAPRPWTAEDSVLVVLAFYTMLSNNDSYERAQGVMHAALPPKLYDFLTPSTSRFDRP
                     VLGSSPVDATGGYAPLPIPGADVVDLRRQRAPARDGVPRIEPPLIGPASNNWVVDWTR
                     STAGRAIVANDPHLSLRLPNIFYRIELEWPQRAARGVSIPGLPGVLIGASDDVAWGAT
                     VSNADQSDWVVVEVDPTDPNRYRTPDGSEPFKTETAEIVVAGREPERLTTRRTRWGPV
                     VGEDWLGRPLALHATWLEPQGLDLDIVGIAAATDVAGASALLARWSGPSLNWVLADRA
                     GDVAWVVNGPIPRRVGFDGSRPESLADGSRSWQGRLPPPGALGGRDGVLFTANSRTLP
                     PDRADAVSRMWMRPLRAKRIDDLLSEQRTFDERDFLRMQLDTRAEGYEQIRATILAVV
                     PADERDPRLEQALELAQNWNGTADVDQPAFRMLHAYYRALTERTLEPLLAPAIAADPT
                     FVYRWPLADEVLRRLLDEQPPNLLTSEHADWNAFL"
     gene            2068..2925
                     /locus_tag="VM692_08075"
     CDS             2068..2925
                     /locus_tag="VM692_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUQ52165.1"
                     /translation="MLKTLLLVLAGIVAGLAAAFWLAPSSAPPAEIAAVGASSRSEPI
                     RATADASIPRLAALEKALASEVEQRTALETKIEELASQLEAVEQRQARAGGPPNFQAG
                     AGGPDPAAVAEMRERFRRDPGARNEEAERRFSEQLVAEGFAPDRAEWIIRRTEELRMQ
                     ALQAQYDAQRGGRPVDPVAGEQSLRTELGEADYERYLRAYGRPTAVPVRDVLASSPAE
                     RSGLHAGDEIVAYAGKRVFDMRDLNALTLEGTPGESVVLEVRRDGQNVQLVMPRGPIG
                     ITGGGFRGR"
     gene            3019..3993
                     /locus_tag="VM692_08080"
     CDS             3019..3993
                     /locus_tag="VM692_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HUQ52166.1"
                     /translation="MSLQEKFGKLEAHLKRLVVGQERLLNRMLVALLSDGHLLVEGAP
                     GLAKTRAIKVLSHSVEGDFHRLQFTPDLLPADLTGTEIYRPQDGSFVFRQGPLFHNLV
                     LADEVNRAPAKVQSALLEAMGERQITVGSVTYPLPPLFMVMATQNPIEQEGTYPLPEA
                     QLDRFLLHTLVTYPDAAGERAILELNRAEQKRSATDEQLFTAPELVTQKEVMAARLQV
                     LEQHLSEPLQEYIVQIVLATRSSERYGDDLRDVLQYGASPRATIALDRCARAHAWLAG
                     RDYVTPEDVQGMAHDVLRHRILLGFEAEAAGRTPNEIVDTLLARIGVP"
     gene            3995..>4207
                     /locus_tag="VM692_08085"
     CDS             3995..>4207
                     /locus_tag="VM692_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUQ52167.1"
                     /translation="MAAPAARTPPSPVQVTLADLVRLRPAGESLKLTAPRIRVATEGG
                     HVSPYKGRGVEFDESRPYQAGDDLRTI"
BASE COUNT          658 a         1445 c         1475 g          629 t
ORIGIN      
        1 aggaacgcgt tccagtccgc gtgctcgctc gtcaggaggt tcgggggctg ctcgtcgagc
       61 agccgccgca ggacctcgtc ggcgagcggc cagcgataga cgaaggtcgg atcggccgcg
      121 atcgcgggcg cgagcagcgg ctcgagcgtg cgctcggtca gcgctcggta gtacgcatgc
      181 agcatgcgga acgccggctg gtcgacgtcg gcggtgccgt tccagttttg cgcgagctcg
      241 agcgcttgct cgaggcgcgg gtcgcgctca tcggccggca cgacggcgag aatcgtcgcg
      301 cgaatctgct cgtagccttc ggcgcgcgtg tcgagctgca tgcgcaagaa gtcgcgctcg
      361 tcgaacgtgc gctgctcgct caacaagtcg tcgatacgct tcgcgcgcag cggccgcatc
      421 cacatgcggc tcacggcgtc tgcgcggtcg ggcggcagcg tgcggctgtt cgccgtgaac
      481 aacacgccgt cgcgcccgcc gagcgcgccg ggcggcggca gccgcccttg ccacgagcgg
      541 ctgccgtcgg ccagcgattc ggggcgcgag ccgtcgaagc cgacgcggcg cgggatgggc
      601 ccattgacga cccaggcgac gtcgccggcg cgatcggcga gcacccagtt cagcgacggc
      661 ccggaccaac gcgcgagcag cgcgctggcg cccgcgacgt cggtcgcggc cgcgatgccg
      721 acgatgtcca agtcgagccc ctgcggctcg agccaggttg cgtgcagtgc cagcggtcgg
      781 ccgagccaat cctcgccaac gacgggcccc cagcgcgtcc gccgcgtcgt cagtcgttcc
      841 ggctctcgac ccgcgacgac gatctcggcg gtttctgtct tgaacggctc tgagccgtcg
      901 ggcgtccgat atcgattcgg atcagtcggg tcgacttcga cgacgaccca gtcgctttga
      961 tccgcgttac tgaccgtcgc gccccacgcg acgtcgtcgc tggcgccgat cagcacgccg
     1021 ggcaacccgg ggatgctgac cccgcgtgct gcgcgctgcg gccactcgag ctcgatgcga
     1081 taaaaaatgt tcggcaaacg caggctcaag tgcgggtcgt tcgcgacgat tgcgcggccc
     1141 gcggtgctgc gcgtccaatc gacgacccag ttgttcgacg cgggcccgat cagcggcggc
     1201 tcgatgcgcg gcacgccgtc gcgtgccggt gcgcgttgcc ggcgcaggtc gacgacgtcg
     1261 gcgcccggga tcggcaacgg cgcgtagccg cccgtcgcgt cgaccggcga gctgccgagc
     1321 acggggcgat cgaagcgcga agtcgacggc gtcaggaagt cgtacaactt aggcggcagc
     1381 gccgcgtgca tgacgccctg cgcccgctcg tacgaatcgt tgttcgacag catcgtgtag
     1441 aacgccagca cgacgagcac gctgtcctcg gcggtccacg gccgcggcgc ggagcccgcg
     1501 agccagtact cgggcggccg cgctcccaag tcggcgaggc ccgcgttgac gccttgcgta
     1561 tacgcttgga gccactcgtt ctgctcggcc ggcagtgacg cgcgtatgcg ccgcgcgagc
     1621 tcgctgaacc cgaacgcgcg ctgactgcga tcgttcgcta gcgcgcgctc gccgaacagc
     1681 tcggcgagct cgccggcgct cgcacggcgc agcaagtcca tctgaaagaa gcgctcctgt
     1741 gcgtgcaggt aaccctggcc gcgcagcgcg tcgaggaacg attcgccgcg cacggtgccg
     1801 atggcgcgcg cgtcgagcgc gatcgtcaac ggcgcctcga gcccaactaa ttgcgcacgg
     1861 ccgctgtggc gtggcagcga ggcggcgacg acggtgtagg cgatggccgc gagtagcaac
     1921 acgagcactg ccacggcggc gagcaaacgc ttcagcaagt cgaaacccct cggcattcgg
     1981 cgtatcgtga cttcgcatta ttgccggtga acgccgtcgg cagcagctag gccgaacacc
     2041 gggcggccga tggtgtaggt gatcaacatg ttgaagacct tgctgctcgt cctggccggg
     2101 atcgtcgccg ggctcgccgc cgcgttctgg ctggcaccga gctccgcgcc gccggcggag
     2161 attgcagccg tcggtgcttc gagtcgcagc gagccgatca gggctaccgc cgatgcctcg
     2221 atcccgcggc tcgcggcgct cgaaaaagcc ctggcctccg aagtcgagca gcgcacggcg
     2281 ctcgaaacga aaatcgagga gctcgcgtcg cagctcgagg ccgtagagca acggcaagcg
     2341 cgtgccggcg gtccgccgaa ttttcaagcg ggggccggcg gacccgaccc cgccgccgtt
     2401 gccgagatgc gcgagcgctt tcggcgcgac cccggcgcgc gcaacgaaga ggccgagcgc
     2461 cgcttttccg agcagctcgt cgccgaaggg tttgcaccgg accgcgccga gtggatcatc
     2521 cgccgcaccg aggagctgcg catgcaggcg ttgcaggcgc agtacgacgc gcagcgcggc
     2581 ggccggcccg tcgaccccgt cgcaggcgag caatcgttgc gcacggagct cggcgaagcc
     2641 gattacgaac gctacttgcg ggcgtacggg cggccgaccg cggtgcccgt gcgcgacgtg
     2701 ctcgcgagct cgccggccga gcgctcgggc ctgcacgctg gcgacgagat cgtcgcctac
     2761 gccggcaagc gcgtgttcga catgcgagat ctcaacgctt tgacgctcga gggcacgccc
     2821 ggcgaatcgg tcgtgctcga ggtgcgccgc gacggacaaa acgtccagct cgtgatgcca
     2881 cgcgggccga tcggcatcac gggcggcggc ttccgcggcc gctgaagttt tctcgcgagc
     2941 cccggcggcg ccggccgcgg gtggttccgc cgtcccgccg tagcctagaa tggcccgctt
     3001 taggtccggc ttaactcaat gtcattacaa gaaaagttcg ggaagctcga agcgcacctg
     3061 aagcgtctcg tcgtcggtca agaacggttg ctgaatcgga tgctcgtcgc gctgttgagc
     3121 gacggtcatc tgctcgtcga aggagcgccc ggcctcgcga agacgcgcgc gatcaaagtg
     3181 ttgagccaca gcgtcgaagg cgacttccat cgcttgcagt tcactccgga cttgctgcca
     3241 gcggacctca cgggcaccga gatctaccgg ccgcaggacg gctcgttcgt ctttcgccag
     3301 gggccgttgt tccacaattt ggtgctggcc gacgaggtca atcgcgcgcc ggccaaagtg
     3361 caatcggcgc tgctcgaggc gatgggcgag cggcagatca cggtcggctc cgtgacgtat
     3421 ccgttaccgc cgttgttcat ggtcatggcg acgcagaacc cgatcgagca agagggcacg
     3481 tatccgctgc ccgaggcgca gctcgatcgc ttcctgctgc acacgctcgt cacgtatccc
     3541 gatgccgcgg gcgagcgcgc gatcctcgag ctgaaccgcg ccgagcagaa gcgctccgcg
     3601 acggacgagc aactgttcac ggcgcccgag ctcgtcacgc aaaaagaagt catggcggcg
     3661 cgcctgcaag tgctcgagca gcatctgtcg gagccgctgc aggagtacat cgtgcagatc
     3721 gtgctcgcga cgcgttcgtc cgagcgctac ggcgacgact tgcgcgacgt gctgcagtac
     3781 ggcgcgagcc cgcgcgcgac gatcgcgctc gatcgctgcg cgcgcgcaca cgcctggctc
     3841 gcgggccgcg attacgtgac gcccgaggac gtgcaaggca tggcgcacga cgtgctgcgg
     3901 caccgcatcc tgctcggttt cgaggccgag gccgcgggcc gcacgccaaa cgagatcgtc
     3961 gacacgctgc tcgcgaggat tggggtgccc tgacatggca gcgcccgccg cgcgtacccc
     4021 gccctcgccg gtgcaggtca cgcttgcgga tttggtgcgt ctgcgaccgg ccggcgagtc
     4081 gctcaagctc acggcgccgc gcatccgcgt ggcgaccgaa ggcggccatg tatcgccgta
     4141 caaaggccgc ggcgtcgagt tcgacgagtc gcggccgtat caggccggcg acgacctgcg
     4201 gacgatc
//
LOCUS       DATLEE010000379        10179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3205320, whole genome shotgun sequence.
ACCESSION   DATLEE010000379 DATLEE010000000
VERSION     DATLEE010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3205320"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..2394
                     /locus_tag="VM692_08090"
     CDS             217..2394
                     /locus_tag="VM692_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUQ52168.1"
                     /translation="MVFLGRWLLQHVLRLAYGVRVTGLENYAQAGKRVLIVANHTSFL
                     DAALLAVFLPDRLTFAVNTEISKRWWMQPLLKIVDAFPLDPTNPYSLKSLIRYIEQDK
                     RAAIFPEGRITVTGSLMKIYPGPGLVADKSGAMVLPVRIDGAQYTPFSRLRGRIRLRW
                     FPLITMTILPPRRLEIPVEIRGRERRRVAGKLLSDLMTEMMFATSNYRRTLYGALLDA
                     RRVHGGKHVIVEDVERVALTYNQLLVRALLLGGLVAAETRRGEAVGVMLPNAIAAVAT
                     LFGLQANGRVAAMLNYTLGAKSLLASCEAAQVRRIYTSERFVVAAKLEHLVDKLREVA
                     TVVYLEDLRERITPWLKLKAALSSRVARLIYTRRRVTPDEAAIVLFTSGSEGLPKGVV
                     LSHANVLANREQVAARIDFGAQDVILNALPMFHSFGLTAGTLLPLLSGMRTFFYPSPL
                     HYRIVPEIAYDVSATIMFGTNTFLAGYARFAHPYDFYSVRYVFAGAEKLKDETRRIWT
                     DKFGVQVFEGYGATETSPGISINTPIDSKPGTVGRLLPGIEHALDPVPGVVGGRLSVR
                     GPNVMLGYLKHDKPGVLQPPATERGPGWYDTGDIVTIDADGFMTIQGRAKRFAKIGGE
                     MVSLTAVEELATRTWPESQHVCISLPDFQKGEQLILVTTRPEAQRTELSARARADGMS
                     ELNVPKRIVHVDAIPLLGSGKADYPAVHALVEATLPHGERSVQ"
     gene            2391..3554
                     /gene="lplT"
                     /locus_tag="VM692_08095"
     CDS             2391..3554
                     /gene="lplT"
                     /locus_tag="VM692_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HUQ52169.1"
                     /translation="MNRGVAVLLGAQFLTAFADNSILWTAVAMLFEAPRGDWYVPALQ
                     ASFLVAFVVLAPWVGVLADRRPKPQVLLIGNVLKAAGAGLMFVGVEPLVAYALVGTGA
                     AVYGPAKYGILPELVGPERLVRANGWIEGSTIVAIVLGSAVGARIADRSVPLALTVVM
                     GCYVTSAAATLVLPRLAAAQVNAEAGLRTFVERMRTLFVVARARFAMLGNSLFWASAA
                     VLRVLLVAWAAKVLATQNSSDVADLTLFLALGIVVGALLVPRLIPIAHLRRARLAAYA
                     MGVTILIFSFVTDWWPARAVLVVIGICGGMFLVPINAALQDIGHRTIGSGRAVALQSF
                     FENSAMLTGVGIYTASVGLGTQPVAALVVVGVFVLVATTVVSWHLPPDPLPEV"
     gene            3652..4437
                     /locus_tag="VM692_08100"
     CDS             3652..4437
                     /locus_tag="VM692_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52170.1"
                     /translation="MSPTLRMLIATAGCVVASAAAEAQWWLDVPTGGVPRTASGEVDV
                     SAPAPRAPDGRPDLSGVWEPIKTYSRDLAEDLVALREIPYQPWAKALVADRADGSESR
                     DDPPAHCLPQGVPRLAGAPAPWKIVQTPALTVIIYEAFTLWRQIFTDGRRLAADVNPT
                     WLGYSSGRWDGDTLVVETRGFNGKSWLDQVGNPSTEQLRVVERYRRPDFGHLEIEITI
                     DDPGAYTAPWTVRQRANLLVDTDLLEFACNENNRDVQHLPGGD"
     gene            4460..4876
                     /locus_tag="VM692_08105"
     CDS             4460..4876
                     /locus_tag="VM692_08105"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52171.1"
                     /translation="MNATSSAALVCTAALVVATSALAHHSFPAQYDINRPITLTGKVT
                     KVEWTNPHIFIYADVKDEKSGEVVNWAFEMGGPNALIRRGWTRNSLKADDLVTFEGSL
                     ARDGSRLVNARTITMTETGRRMFAGSSGGDEPPTPQ"
     gene            complement(4880..5311)
                     /locus_tag="VM692_08110"
     CDS             complement(4880..5311)
                     /locus_tag="VM692_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ52172.1"
                     /translation="MRARWLAAAALLLAGAGIQAQSLVETRRAEVFAAERAFARSMAD
                     RDFAAFGRYIAEDSVFFSGTTTLRGRAAVLAGWEPLFAERAAPFSWEPDQVEVLESGE
                     LALSTGLVKNPEGVVSARFNSIWRRQPDGRWLVVFDKGGPP"
     gene            complement(5313..6044)
                     /locus_tag="VM692_08115"
     CDS             complement(5313..6044)
                     /locus_tag="VM692_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52173.1"
                     /translation="MAATAATLPRTRAPRRLYVALAVLAALIAAVGFWPTYFGPLLAG
                     AADKTSIIHFHAAVYVGWLAIFITQAALAATGRVATHIKLGRFAIGYGVLVIVVGLVT
                     AFARFAVRVRAGEAAAAQAQLLGPLIDMLVFAPLFGAAIHYRRAPELHKRLMIVATTS
                     LLIAAVARMPILGNPPDRLLLHLIWTAPILLAIAHDFWRQRKVHPIYVLGLIVLVLEG
                     PLVRTPARQSQLWLDLSRWLVDWVA"
     gene            complement(6051..6461)
                     /locus_tag="VM692_08120"
     CDS             complement(6051..6461)
                     /locus_tag="VM692_08120"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52174.1"
                     /translation="MSRLSFAAIAAVFAICPMTASQGHHSFAAEYDRDKPVELTGAVT
                     KVEWTNPHARIYIDVKDADGKVVNWDFELGPPNGLMRQGWRRDSLREGHVVTINGFLS
                     RDQEHVANARSVKLPDGRQVFAGSSFDVDAAAAQ"
     gene            6558..7370
                     /locus_tag="VM692_08125"
     CDS             6558..7370
                     /locus_tag="VM692_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52175.1"
                     /translation="MTRLLRICAAFALLMPLTVAAQWDAHLNSRTPRKADGTPDLEAP
                     APRTADGKPDFTGVWQGFGTLGGTAAQAEPEGSVPRAGFANVAQNMKDPPLPIRPEAA
                     AIAEARRAAGGKDNPEAICLPMGIMQFHTQGAPRKFIQTPDVLVILYEASMGVRQIFT
                     DGREFPTDNPQPWWYGYSVGRWDGDTLVVTTKGLRGDEWLDIFGTPLSDAATYTERFR
                     RVSFGRMEIDVTVDDPKNYTAPWTVRVNQRLMPDAELLEFVCNENNRYFVRP"
     gene            7384..8877
                     /locus_tag="VM692_08130"
     CDS             7384..8877
                     /locus_tag="VM692_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52176.1"
                     /translation="MRNTKSCVRFTLVALACGAAAACDTAQAPPTVAVAQAAQPDDPF
                     APHDLKLVGQNDLQARSAYQPVVHAYGTRRILFVGHHAGEALNLATGYLEKNGLSILD
                     VTDPAKPQYLAHMPPTGPEASGTQHVQVCDGSALPNGDDAKVYAIRTNGLLGYEVIDV
                     TDPASPEFLRTIAETGTSSRPESERGNRETHKFQWDCATGIAYMNGTAPGWRVTRVLQ
                     AFDLSDPNAPKHIRDFGLAGYEPTATGPYPEPQVAGLHQPFVVGNRMYLGYNSGEEGV
                     LQIVDRDAFLRGDATPTAASLQAPQIARIDLPSYWGVHTAKPIYGFEIADYADNSEAR
                     TRDLLLVSSEAGTFRCQEPRDVLLIFDITEERKPLAVASFQVPEEPGDFCHRGGRFGP
                     HSFADAYHPDFDKKLVVLAYFNAGVRVVDIRNPFEPKEVARFIPEVTANTTESCIEIG
                     GTRQCDRAIQTNNVNIDDRGYIYAVDRASTGLHVLELTGAARAIAGF"
     gene            8895..9971
                     /locus_tag="VM692_08135"
     CDS             8895..9971
                     /locus_tag="VM692_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HUQ52177.1"
                     /translation="MSKRWVAAIAALAASSAVQAQRNPPEIPYESVPNFFQLPPDTYF
                     GEVAGIAVNSQKHIFVFSRGGTTGPAYGAAAAQLFEFDEHGAFIREIGKGLYAWSFAH
                     TVRIDRHDDIWAADKGSDMVVRFDTAGRVKMVFGRKPEASNEEAHPLARPNPPLPPID
                     QQFRQVTDVAWDSKDNIYITDGYVNARVARYDVNGDWLGSFGEPGTGPGQYNTLHSIV
                     IDNQDRLWIADRNNRRIQVTDTTGKVLKIVTIDVPAPPNAPVATGNRLPATGGQTQQM
                     PGSPWAICITPGPNQVLYVSDAYPGRIYKLSLEGEVLGYFGGVGKTPGKFGWIHELAC
                     PSENELYVAELLNWRAQKLVLKPR"
BASE COUNT         1716 a         3348 c         3423 g         1692 t
ORIGIN      
        1 ctcggcgtcg acttcgtcca gggcttcgcc gtcgaaaagc cgcggccgct acaggccgtc
       61 cattaacggc tcggtgccgg tgctgcggtt cggttgaacc gctcggtgcg gttacggttc
      121 gctcgaaccg cagccgtcgc accggcaccg cgatggcaat tggcggccgc tgcgggcatc
      181 gttatgcctc cgtaatcgta cggcgaggcg gctcgaatgg tgttcctggg tcggtggttg
      241 ttgcagcacg tcctgcgcct cgcctatggc gtccgcgtca cgggcctcga gaactatgcg
      301 caggccggca agcgcgtgct gatcgtcgcc aaccacacct cgttcctcga cgcggcgctg
      361 ctggccgtct tcctgccgga taggctcacc ttcgcggtca acacggagat ctcgaagcgc
      421 tggtggatgc agccgctgct gaagatcgtc gacgcgtttc cgctcgatcc gacgaatccc
      481 tactcgctga aatcgttgat ccgctatatc gagcaagaca agcgcgcggc gatcttcccc
      541 gaagggcgca tcacggtcac gggctcgttg atgaagatct acccgggccc gggcctcgtc
      601 gccgacaaat cgggcgccat ggtgctgccg gtacgcatcg acggggcgca gtacacgccg
      661 ttttcgcgcc tgcgcggccg cattcggctg cgttggttcc cgctgatcac gatgacgatc
      721 ctgccgccgc ggcggctcga gattccggtt gaaattcgcg gtcgcgaacg gcgccgcgtc
      781 gcgggcaagc tgttgtcgga cctcatgacc gagatgatgt tcgcgacatc gaactaccgt
      841 cgcacgctgt acggcgcgct gctcgatgcg cgccgcgtgc acggcggtaa gcacgtcatc
      901 gtcgaggacg tcgagcgcgt tgcgctgacg tacaaccagc tgctcgtgcg ggcgctgctg
      961 ctcggcggcc tcgtcgccgc cgagacgcgg cgtggcgagg ccgtcggcgt catgctgccg
     1021 aacgcgattg cggccgtggc gacgttgttc ggcttacagg cgaacggccg cgtggccgcg
     1081 atgctgaact acacgctggg cgcgaagagc ttgctcgcgt cgtgcgaagc cgcgcaggtc
     1141 cgccgaatct acacgtcgga gcgcttcgtc gtcgccgcca agctcgagca tctcgtcgac
     1201 aagctgcgcg aggtggcgac cgtcgtttac ctcgaggact tgcgcgagcg cattactcct
     1261 tggctcaagc tcaaagccgc cctgtcgagc cgcgtggcgc ggctcatcta cacgcgccgg
     1321 cgggtgacgc cggacgaagc ggcgatcgtg ttgttcacgt cgggctccga agggttgccg
     1381 aaaggcgtcg tgttgtcgca cgccaacgtg ctcgcgaacc gcgagcaagt cgccgcgcgg
     1441 atcgacttcg gcgcgcagga cgtcattctc aacgcgctgc cgatgttcca ttcgttcggg
     1501 ctcacggccg gcacgctgct gccgctgttg tccggcatgc gcacgttctt ttatccgtcg
     1561 ccgctgcact accgcatcgt tcccgagatc gcgtacgacg tcagcgcgac gatcatgttc
     1621 gggacgaaca cgtttctcgc gggctacgcg cggtttgcgc atccgtacga tttctacagc
     1681 gttcgctacg ttttcgcggg cgcggaaaag ctcaaagacg agacgcgccg catttggacc
     1741 gacaagttcg gcgtgcaagt cttcgaaggc tacggcgcga ccgagacgag ccccggcatc
     1801 tcgatcaaca cgccgatcga ttcgaagccc ggcaccgtcg ggcgcttgct acccggcatc
     1861 gagcacgcgc tcgatcccgt gccgggagtc gtcggcggac gactgtccgt gcgcggcccg
     1921 aacgtcatgc tcgggtattt gaagcacgac aagcccggcg tgttgcagcc gccggcgacg
     1981 gaacgcgggc ccggttggta cgacacgggc gacatcgtca cgatcgacgc cgacgggttc
     2041 atgacgatcc agggccgcgc caagcgtttt gcgaagatcg gcggcgagat ggtgtcgctg
     2101 acggccgtcg aggagctcgc gacgcgcacc tggcccgagt cgcagcacgt ctgcatcagc
     2161 ttgcccgatt tccagaaagg cgagcagctg attctcgtga cgacgcggcc cgaagcgcag
     2221 cgaaccgaat tgtcggcgcg cgctcgagca gacggcatga gcgagctcaa cgtgccgaag
     2281 cgcatcgtgc acgtcgacgc cattccgctg ctcggcagcg gcaaggccga ctatccggcc
     2341 gtgcacgcgc tcgtcgaagc gacgttgccg cacggcgaac ggagcgtgca gtgaaccgcg
     2401 gcgttgctgt cctgctcggc gcgcagttcc tgactgcgtt cgcggacaac tcaatcctgt
     2461 ggacggcggt cgcgatgttg ttcgaggcgc cgcgcggcga ctggtacgtg ccggcgctgc
     2521 aggcctcgtt cctcgtcgcg ttcgtcgtgc tggcgccatg ggttggcgtc ctcgccgacc
     2581 ggcggccgaa gccgcaggtg ctgctgatcg gcaacgtgct gaaggcggcg ggcgcggggc
     2641 tgatgttcgt cggggtcgag ccgctcgtag cgtatgcgct agtcggcacc ggcgccgccg
     2701 tctacgggcc ggcgaagtac ggcatcttgc ccgagctcgt cgggcctgag cggctagtgc
     2761 gcgcgaacgg ctggatcgag ggctcgacga tcgtcgcgat cgtgctcggc tcggcagtcg
     2821 gcgcgcgcat cgcggaccgc tccgtaccgc tcgcactgac ggtcgtcatg ggctgctacg
     2881 tcacgtcggc cgccgcaacg ctcgtgttgc cacgactggc agccgcccag gtcaatgccg
     2941 aggccgggct tcgaacgttc gtcgagcgga tgcgcacctt gttcgtcgtc gcgcgcgcgc
     3001 ggttcgcgat gctcggcaac tcgctgttct gggcctccgc agccgtgctg cgcgtgctgc
     3061 tcgtcgcgtg ggccgccaag gtgctggcga cgcagaactc gagcgacgtc gccgatctca
     3121 ccttgttcct cgcgctcggc atcgtcgtcg gcgcgctgct cgtgccgcgc ctcataccga
     3181 tcgcgcactt gcgccgcgca cgactcgccg cctacgcgat gggtgtcacg atcttgatct
     3241 tcagcttcgt caccgattgg tggcccgcgc gcgccgtgct cgtcgtcatc ggcatctgcg
     3301 gcggcatgtt tctcgtgccg atcaacgccg cactgcagga catcgggcac cgcacgatcg
     3361 gcagcgggcg cgccgtcgcg ctgcagagct tcttcgagaa ttccgcgatg ttgacgggcg
     3421 tcggaatcta tacggcatcg gtcgggctcg gcactcagcc agttgcggcg ctcgtcgtcg
     3481 tgggcgtgtt cgtgctggtt gcgacgacgg tcgtctcgtg gcatctgccg ccggatccgc
     3541 tgccggaggt ttgagcgcag cgccctgcga tcgcgtaccg cgggccccct ttgacagatg
     3601 ggcgatcgag acctagggtg aggccgagca gtggcggaag gggagtcgcc tatgtcgccg
     3661 acgttacgga tgctgatcgc aaccgctggt tgcgtcgtcg caagcgccgc cgccgaagcg
     3721 cagtggtggc tcgacgtccc gaccggcggc gtgccgcgca ccgcgagcgg tgaggttgac
     3781 gtgtcggcgc ccgcgccgcg agcccccgac ggccgtcccg atctgtcggg cgtctgggag
     3841 ccgatcaaga cgtattcgcg cgacctcgcc gaggatctcg tcgcgctgcg cgaaataccg
     3901 tatcagccgt gggccaaggc gctcgttgcc gatcgcgccg acggctcgga atcgcgtgac
     3961 gatccgcccg cgcactgttt gccgcaggga gttccgcgtc tcgccggcgc tccggcgcct
     4021 tggaagatcg tgcagacgcc ggcgctgacg gtcatcatct atgaagcgtt caccctgtgg
     4081 cggcagatct tcaccgacgg ccggcggctc gcggccgacg tcaatccgac gtggctcggt
     4141 tactcgagcg gccgctggga cggcgatacg ctcgtcgtcg agacgcgcgg cttcaacggc
     4201 aagtcatggc tcgatcaggt cggcaatccg tcgaccgagc agctgcgcgt cgtcgagcgc
     4261 tatcggcgtc cggacttcgg tcatctcgag atcgagataa cgatcgatga cccgggcgct
     4321 tacacggcgc catggacggt gcggcagcgc gcgaacctgt tggtggacac cgatctgctc
     4381 gagttcgcgt gcaacgagaa caaccgcgat gtacaacatc tgccgggcgg cgactgacgg
     4441 caggcttcgg gaggtatcca tgaacgctac ttcgtcggcg gctctcgtgt gcacggcggc
     4501 ccttgtcgtc gccacgagcg cgctcgcgca tcactcgttt cctgcgcagt acgacatcaa
     4561 cagaccgatc acgctgacgg gcaaggtgac gaaggtcgag tggaccaatc cgcacatctt
     4621 catctatgcg gacgtcaagg acgagaagtc cggcgaagtc gtgaactggg cgttcgaaat
     4681 gggcgggcca aacgccttga tccgccgcgg ctggacacgc aactctctga aggccgacga
     4741 cctcgtgacg ttcgaaggca gtttggcgcg cgacggcagc cgcctcgtca acgcgcgcac
     4801 gatcacgatg accgaaacgg gtaggcggat gttcgcgggc tcgagcggcg gggacgagcc
     4861 gcctacgccg caatgagagc tacggcggcc cgcctttatc gaagacgaca agccagcggc
     4921 catccggctg ccgccgccag atcgagttga aacgcgccga cacgacgcct tcggggtttt
     4981 tgacgaggcc cgtggaaagc gcaagctcgc ccgattccaa gacctcgact tgatccggct
     5041 cccacgagaa cggcgccgct cgttcggcga acaacggctc ccagccggcg aggacggcgg
     5101 cgcgcccgcg cagcgtcgtc gtgcctgaaa agaacaccga gtcttcggcg atgtagcggc
     5161 cgaacgccgc gaagtcgcgg tcggccatgg agcgcgcgaa cgcgcgctcg gcggcgaaga
     5221 cttcggcgcg ccgcgtctcg acgaggctct gcgcttgaat gcccgcgccg gccaagagca
     5281 acgcagcggc cgcgagccaa cgcgcgcgca tgctaggcca cccagtccac gagccagcgg
     5341 ctcaagtcga gccacagctg cgattggcgc gccggcgtgc ggacgagggg cccttcgagc
     5401 acgaggacga tgaggccgag cacatagatc gggtgcactt tgcgctgacg ccaaaagtca
     5461 tgcgcgatcg cgagcaggat cggtgccgtc caaatcaggt gcaacagcag ccggtcgggt
     5521 gggttgccga gaatcggcat gcgagccacg gccgcaatca gcagtgacgt cgtcgcgaca
     5581 atcatcaggc gtttgtgcag ctcgggagca cgccgataat ggatcgccgc gccgaacagc
     5641 ggcgcaaaca ccagcatgtc gatgagcggc cctagcagct gcgcctgcgc cgcggcggct
     5701 tcgccggcgc gcactcgcac ggcaaaccga gcgaacgccg tgaccagccc gaccacgatc
     5761 acgagcacgc cgtagccgat cgcgaaacgg ccgagcttga tgtgcgtcgc gacgcggccg
     5821 gtcgctgcga gcgcagcttg cgtgatgaag atcgcgagcc agccgacgta gacggccgcg
     5881 tgaaaatgaa taatcgacgt cttgtcggcg gcgccggcga gcaagggacc gaagtacgta
     5941 ggccagaagc cgaccgcggc gatcaacgcc gcgagaacgg ccagcgcgac gtacaaccgg
     6001 cgcggcgcac gcgttcgagg gagcgtggcc gccgtcgccg ccatcggtcg ctattgtgcg
     6061 gcggcggcgt cgacgtcgaa cgacgagccc gcgaacacct ggcggccgtc gggcagcttc
     6121 acggagcgcg cgttggcgac gtgctcttgg tcgcgcgaca agaagccgtt gatcgtcacg
     6181 acatggcctt cgcgcagcga gtcgcgtcgc catccttggc gcatcaagcc gttcggcggg
     6241 ccgagctcga aatcccagtt gacgactttg ccgtcggcgt ctttgacgtc gatatagatg
     6301 cgggcgtgcg gattcgtcca ctcgaccttg gtcacggcgc ctgtcagctc gacgggcttg
     6361 tcgcggtcgt actcggcggc gaacgagtga tgaccctggc tcgcggtcat ggggcagatt
     6421 gcgaagactg cggcgatcgc ggcgaacgac aaacggctca tcgatgcttt ccccctcacg
     6481 acttcccggc gatgatacgc tgagtaagcc cgctacgaac attccagtcc ggaccatcga
     6541 gagcaagaag aatgatgatg actaggctgc ttcgtatctg cgctgcgttt gcactgttga
     6601 tgccgttgac cgtcgccgcg cagtgggacg cgcacctgaa ttcgcggacg ccgcgcaaag
     6661 cagacggtac gcccgatctc gaagcgccgg ctcctcggac ggctgacggc aagccggatt
     6721 tcacgggcgt ttggcagggc ttcggcacgc tcggcggcac ggcggcgcag gccgagcccg
     6781 agggctcggt gccgcgcgcg gggttcgcga acgtcgcgca gaacatgaag gatccgccgc
     6841 tgccgattcg ccccgaggcc gcggccatcg cagaagcgcg gcgcgccgcc ggcggcaagg
     6901 acaaccccga ggccatctgc ctgccgatgg gtatcatgca attccacacg caaggcgctc
     6961 cgcgcaagtt cattcaaacg ccggacgtgc tcgtgatcct ctacgaggcg agcatgggcg
     7021 ttcgccaaat cttcaccgac ggccgcgagt ttccgaccga caatccgcag ccgtggtggt
     7081 acggctattc ggtcggccgt tgggatggcg acacgctcgt cgttacgacg aaaggtttgc
     7141 gcggcgacga gtggctcgac atattcggaa cgccgttgtc ggacgcggcg acctataccg
     7201 agcggtttcg ccgcgtgagc ttcgggcgta tggagatcga cgtgaccgtc gacgacccga
     7261 agaactacac ggccccttgg accgtgcgcg tcaatcaacg cctcatgccc gacgccgagc
     7321 tgctcgagtt cgtttgcaac gaaaacaatc gctacttcgt gcgtccctga cggagcaagc
     7381 gcgatgcgca acaccaagag ctgcgtcagg ttcaccctcg tagcgcttgc gtgcggcgcc
     7441 gctgccgcct gcgataccgc gcaagccccg ccgacggttg ccgtcgcgca ggcggcgcag
     7501 ccagacgatc cgttcgcgcc gcacgaccta aagctcgtag gccagaatga cttgcaagcg
     7561 cgctccgcat accaaccggt cgtgcacgcc tatggcacgc ggcgaattct gttcgtgggc
     7621 caccacgccg gcgaggcgtt gaatttggcg acgggctacc tcgagaaaaa cggcctctcg
     7681 atcctcgacg tcacggatcc ggcgaagccg caatacttgg cgcacatgcc gccgacgggg
     7741 cccgaggcca gcggcacgca gcacgtgcaa gtctgcgacg gcagtgcgct gccgaacggc
     7801 gacgacgcga aggtctacgc gattcgcacc aacgggcttc tcggctacga ggtcatcgac
     7861 gtcacggatc cggctagccc tgagttcctg cgcacgatcg ccgagacggg cacgtcgtcg
     7921 cggccggagt ccgagcgcgg caaccgagag acgcacaagt ttcagtggga ctgcgcgacc
     7981 ggcatcgcct acatgaacgg cacggccccc ggctggcggg tcacgcgcgt gctgcaagcg
     8041 ttcgacttga gcgatcccaa tgcgccgaag cacatccgcg atttcgggct cgcgggttac
     8101 gagccgaccg cgacgggtcc atatccagag ccgcaggtcg cggggttgca tcaaccgttc
     8161 gtcgtcggca accgcatgta tttgggttac aacagcggcg aggagggcgt gctgcagatc
     8221 gtcgatcgcg acgccttcct gcgcggcgat gcgacgccga ctgcggccag cctgcaagcg
     8281 ccgcaaatcg cgcgtatcga cttgccgagc tactggggcg tgcatacggc aaagccgatc
     8341 tacggtttcg agatcgcgga ctacgccgac aacagcgagg cgcgcacgcg agacctgctg
     8401 ctcgtctcga gcgaagccgg cacgttccgt tgccaggagc cgcgcgacgt gctgctgatc
     8461 ttcgacatca ccgaggagcg caagccgttg gccgtagcga gcttccaagt gcccgaagag
     8521 cccggcgact tctgtcatcg cggcggccgt ttcggcccgc attcgttcgc cgacgcctac
     8581 caccctgact tcgacaagaa gctcgtcgtg ctcgcgtact tcaacgccgg cgtgcgcgtc
     8641 gtcgacattc gcaacccgtt cgagccgaag gaagtggcgc gcttcattcc cgaggtgacg
     8701 gcgaacacga ccgagtcgtg catcgagatc ggcggcacgc ggcagtgcga tcgcgcgatt
     8761 caaacgaaca acgtcaacat cgacgatcgc ggctacatct acgcggtcga tcgcgcctcg
     8821 acgggcctgc acgtgctcga gcttacgggt gcagcgcgcg cgatcgcggg tttttgaaca
     8881 aaggggagac tgtcatgagc aagcgatggg ttgcggccat agccgcgttg gcggcgtcgt
     8941 cggcggttca agcgcagcgg aacccgcccg agatcccgta cgagtccgtg ccgaacttct
     9001 tccagctgcc gccggatacg tacttcggcg aggtggccgg catcgccgtg aactcgcaga
     9061 agcatatctt cgtgttctcg cgcggcggca cgacggggcc cgcgtacggc gcggctgcgg
     9121 cacaactgtt cgagttcgac gagcacggcg cgttcatccg cgagataggc aaaggcctgt
     9181 acgcgtggtc gttcgcgcac acggtgcgca tcgatcgcca cgacgacatc tgggccgcgg
     9241 acaagggctc ggacatggtc gtgcggttcg acaccgcggg ccgcgtgaag atggtgttcg
     9301 gccgcaagcc cgaagcgtcg aacgaggaag cacacccgct cgcgagaccg aatccgccgc
     9361 tgccgccgat cgatcagcaa tttcgtcagg tgaccgacgt cgcctgggac tcgaaggaca
     9421 acatctacat cacggacggc tacgtgaacg cgcgcgtcgc gcgttacgac gtcaacggcg
     9481 actggctcgg ctcgttcggc gagccgggga cggggccggg ccaatacaac acgctgcact
     9541 cgatcgtcat cgacaaccaa gatcggctgt ggatcgcgga tcgcaacaat cggcgcattc
     9601 aagtcacgga cacgacgggc aaggtgctga agatcgtcac gatcgacgtg ccggcgccgc
     9661 cgaatgcgcc cgttgcgacg ggcaatcgct tgcccgcgac cggcggccag acgcagcaaa
     9721 tgccgggctc gccgtgggcg atttgcatta cgccggggcc gaatcaggtg ctgtacgttt
     9781 ccgacgcgta cccgggccgc atctacaagc tgtcgctcga aggcgaggtg ctcggctatt
     9841 tcggcggcgt cggcaagacg ccgggcaagt tcggctggat ccacgagctc gcgtgcccgt
     9901 cggagaacga gctctacgtc gcggagctcc tgaactggcg cgcgcaaaag ctcgtgctga
     9961 agccgcgcta gtcgttctgc ggcgaggcga cgggggccgg cgtctccgtc gtgtcgaaca
    10021 cgcccttgtg atggaagtag atgatgccgg ccgcggcgcg catcggcgcc gtcgtcgtgc
    10081 cgttcaccac gttctccacc gcgcgcgaac tgcgcgaaca gctggccgac agcgacgtcg
    10141 ccgtcctgct ggccgtcgag agctatcgct cgcacgact
//
LOCUS       DATLEE010000380         6275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4131743, whole genome shotgun sequence.
ACCESSION   DATLEE010000380 DATLEE010000000
VERSION     DATLEE010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4131743"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VM692_08140"
     CDS             complement(<1..381)
                     /locus_tag="VM692_08140"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HUQ52178.1"
                     /translation="MSSPDTHDDDHDPWKSPQGDAPWKSPEGDDHQAVPSDAALRAKA
                     LESLLVEKGLVDAAALDALVDTFENKVGPRNGARVVARAWTDPDYKARLLADGTVAIA
                     ELGFHGQQGEKLTVVENTPSVHNVL"
     gene            489..1304
                     /locus_tag="VM692_08145"
     CDS             489..1304
                     /locus_tag="VM692_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HUQ52179.1"
                     /translation="MSADPSVRSELTTAVFVDFENLALGVEQDKKGRFEIELVLKRLL
                     ERGRIVFKRAYCDWSRFQNFMYEFHRYGFEMVDIPSSKVSGKNSADIRMVVDAIDLCY
                     SKSHIDTFVLVTGDSDFSPLVSKLKENDKRVIGCGVRSSTSDLLANNCDEFIFYDDLI
                     RVQKRTQQRTQQRRKPKKETAENAETTDGTDKRKQEAFDRLVATVASLETDYDPVWGS
                     LVKPTLKRVYPGFNESYYGYTTFAELLQDAAKEGVIHLELDEQRGNYRVKSVH"
     gene            1416..1742
                     /locus_tag="VM692_08150"
     CDS             1416..1742
                     /locus_tag="VM692_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52180.1"
                     /translation="MSQQGSKAVKDGVQVIQGTPTAPLKSATLFLRLWKVTEGKQTRT
                     RATLFMVRPGPTDYVIKELIPDMELDAQAALEKAIAIAKRGDASVVYLNADLARIPKL
                     RGFVTA"
     gene            complement(1765..2409)
                     /locus_tag="VM692_08155"
     CDS             complement(1765..2409)
                     /locus_tag="VM692_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HUQ52181.1"
                     /translation="MLLKIYRRARQSSFGWLAALAVGCGSAIGAAAQEPMTGSLLVAS
                     PELTDPMYAHAVILVLRHDDEGTIGVVINRPTNLVPSTIFPELKQSIGAYTGNLFRGG
                     PIAPTRLLYVVRGLAAATVNGPEVLDKVFLSVDDDSLPDMTRLADGTNDLRFYAGHAA
                     WVPGQLESEINAGGWEILPASEDLVFHADPGSLWKELEGRGATGGGVVAALGAD"
     gene            2555..3613
                     /locus_tag="VM692_08160"
     CDS             2555..3613
                     /locus_tag="VM692_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HUQ52182.1"
                     /translation="MPAEWEPHEATWLSWPHNRDSWPGVFAGVDPAMVEFVRALAQCE
                     PVYINVLGADHERHVRQLLAAAVPPERLKLFRFPTNDAWARDHGAIFVTRPTADSPRL
                     AVDFDYNAWGGKYPPFDLDRQIGRLMAEALRVPRYAQPGIVLEGGSIEVNGAGALLTT
                     EQCLLNPNRNPTLTRADLERLLRDSFGVREILWLGEGIEGDDTDGHIDDLTRFVGATK
                     VVTVVESNRADPNHAPLAANRRLLDALTVGGRPLEVAELPMPVPQYLEDQRLPASYAN
                     FYLANGAVLVPVFGCPADDTACGILGDCFPGRRVVPIDCRVLIAGLGALHCMTQQVPI
                     EPIVSREVIGVVGRGSIS"
     gene            3660..4631
                     /locus_tag="VM692_08165"
     CDS             3660..4631
                     /locus_tag="VM692_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HUQ52183.1"
                     /translation="MGAISDFIRHHYRHFNGAALVDAADAYVKHLDAGGQMLVTLAGA
                     MSTAELGLSLAEMIRRGKVHAICCTGANLEEDVFNLVAHDHYVRIPNYRYLSPEQEEE
                     LLDQHLNRVTDTCIPEHEAIRRIEKAVLDEWQRADKAGQKFFPHEFMYKILRSGVLEE
                     HYQIDPQDSWLLAAAERDLPMFVPGWEDSTLGNIFAGHCIAGDVDNVHTVRTGIEYMM
                     VLADWYQTTCRKGSIGFFQIGGGIAGDFPICVVPMLQQDLQLPDTPRWGYFCQISDST
                     TSYGSYSGAVPNEKITWGKLSASTPKFIIESDATIVAPLMFAHVLGE"
     gene            4637..>6275
                     /gene="speA"
                     /locus_tag="VM692_08170"
     CDS             4637..>6275
                     /gene="speA"
                     /locus_tag="VM692_08170"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HUQ52184.1"
                     /translation="MSTQPQTKPSTVEWQPSDASDLYRIPSWGDPFFFVNDKGHMAVR
                     ALDEHGTTMDVVDIVNELRRRGVQFPVLLRFQDVLRAQVRRVNEAFRSAIAESNYGNV
                     YRGIYPIKVNQLHEVVDELLDAGRPYGMGLECGSKAELIACLPHIDGETLLVCNGVKD
                     RTMLSLMIAGQRLGQNIVPVMEKYSEFGDVKGLAFSTGYMSQLGVRVRLATRGAGRWA
                     ESSGTNSKFGLNVAELMRMVNELEASGQQSKLVLLHCHIGSQIADIQVLKEATKEIAQ
                     IYAQLVKRGIALKYLDLGGGLGVNYDKGHLEEDAGINYALEEYANAVVFTVKEVCDAN
                     EVPTPTLVTESGRALTAHHSVLIVPVLGAHAKDDPTQDLTVPENAAEPLHALTKILTA
                     LPKTRKQSELLEAYHDINERLEEVRQLFTLGYLPLAERAIAESLYWRICTGLLKALKR
                     GKHDGSRPEIIELEDKLTELYLCDFSVFQSMLDHWAIGQPFPIVPIDRLDEQPEKRAI
                     LVDLTCDSDGKVTHYVSTRDNRFLPVHPTVNGTPYYLG"
BASE COUNT         1120 a         2082 c         2030 g         1043 t
ORIGIN      
        1 gagcacgttg tgcacgctcg gcgtgttctc gacgaccgtc agcttctcgc cttgttgacc
       61 gtggaacccg agctccgcga tcgcgacagt gccgtccgcg agcagccgcg ctttgtagtc
      121 ggggtcggtc caggcgcgtg cgacgacgcg cgcgccgttg cgcgggccga ccttgttctc
      181 gaatgtatcg acgagcgcgt cgagcgccgc tgcgtcgacg aggcctttct cgacgagcaa
      241 cgactcgagc gctttcgcgc gcagcgcggc gtcggacggc acggcctgat ggtcgtcccc
      301 ctccggggac ttccaagggg cgtccccctg cggggacttc caagggtcgt gatcgtcgtc
      361 atgcgtgtcc ggcgagctca tttcggttcc gaccatacca ccgcgagatt cgggcggaca
      421 gacgcaagct tgccaaagcc gctcgcggtc actcactatc gcgggactca agccagcaag
      481 gtattttgat gtccgctgat ccttccgtac gtagcgagct gacgaccgcc gtgttcgtcg
      541 atttcgagaa cttggcgctc ggcgtcgagc aggacaagaa gggccgcttc gagatcgagc
      601 tcgtgttgaa acgcttgctc gagcgcggcc gcatcgtctt caagcgcgcg tactgcgact
      661 ggtcgcggtt ccagaacttc atgtacgagt tccaccgcta cggcttcgag atggtcgaca
      721 tcccgagctc caaggtgtcg ggcaagaaca gcgccgacat ccgcatggtc gtcgacgcga
      781 tcgacctctg ctattcgaaa tcgcacatcg acacgttcgt cctcgtcacg ggcgacagcg
      841 acttctcgcc gctcgtctcg aagctcaagg aaaacgataa gcgcgtcatc ggctgcggcg
      901 tacgcagctc gacgtcggac ctgctcgcca acaattgcga cgagtttatc ttctacgacg
      961 acctgatccg cgtgcagaag cgcacccagc agcgcacgca gcaacgccgc aagccgaaga
     1021 aggagaccgc ggaaaacgcc gagacgacgg acggcacgga caagcgcaag caagaagcgt
     1081 tcgaccggct cgtcgcgacc gtcgcgtccc tcgagacgga ttacgacccc gtttggggct
     1141 cgctcgtgaa gccgacgttg aaacgcgtgt accccggttt taacgagagc tactacggct
     1201 acacgacgtt cgcggagcta ctgcaagacg cggccaagga aggcgtcatc cacctcgagc
     1261 tcgacgagca gcgcggcaac tatcgcgtca agtcggtgca ctgagcgtac gctgtcggcc
     1321 cgccgcgacg cggctttgat acgtccgtca cagcggcgcc ttcgagcgcc gcggagactt
     1381 gaggcttcca aggatggtcg agccgcgagt cagccatgtc gcagcagggg tcgaaggccg
     1441 tcaaagacgg cgtgcaagtg attcagggga cgccgacggc gccgctgaag agcgccacct
     1501 tgttcctgcg cctctggaag gtcaccgagg gcaagcagac gcggacgcgg gcgacgctgt
     1561 tcatggtacg cccgggcccc acggactacg tcatcaaaga gctgattccc gacatggagc
     1621 tcgacgcgca agccgcgctc gagaaggcga tcgcgatcgc caaacgcggc gacgcctcgg
     1681 tggtctatct gaacgccgac ctggcccgca tcccgaagct gcgcggcttc gtcacggcgt
     1741 gaggcaggcg cagcgacgct gacgtcagtc ggcgccgagc gcggctacga cgccgccgcc
     1801 ggtcgcgccg cggccttcga gctccttcca gagactgccg ggatccgcgt ggaagacgag
     1861 gtcttcgctc gccggcagta tctcccagcc gccggcattg atctcggatt cgagctggcc
     1921 cggcacccag gccgcatggc cggcatagaa ccgcaggtca ttcgtgccgt cggccaagcg
     1981 cgtcatgtcg ggcaacgagt cgtcgtcgac gctcaagaac accttgtcga gcacctcggg
     2041 cccgttgacc gtcgccgccg cgagcccgcg cacgacgtac agcaggcgcg tcggcgcgat
     2101 cggcccgccg cggaacaagt tgccggtgta ggcgccgatg ctctgcttga gctccgggaa
     2161 gatcgtgctc ggcacgaggt tcgtcggccg attgatgacc acgccgatcg tgccttcgtc
     2221 atcgtgccgt aacacgagta tcacggcgtg agcgtacatg gggtccgtca gctcgggcga
     2281 agcgacgagc agcgagcccg tcatcggctc ttgggccgcg gctccgattg cgctgccgca
     2341 accgacggca agcgcagcga gccagccgaa cgacgactgc cgagcgcgac ggtaaatctt
     2401 cagcaacatc gcgccatgat aaccgcgttc gacgccgcgc aggtgtaccg attcccatga
     2461 tgaacgcgta tgatgcgcgc cctcttcggg cggcacctca ggagacgcct tgtccgccga
     2521 ccacgcgaac acgccgagcg ctcgtggcta tcggatgccg gccgaatggg agccgcacga
     2581 agcgacgtgg ctgtcgtggc cgcacaatcg cgacagctgg cccggtgtgt tcgccggcgt
     2641 cgatcccgcg atggtggagt tcgtgcgcgc gctcgcgcaa tgcgagcccg tctatatcaa
     2701 tgtgctcggc gccgaccacg agcgtcacgt ccgccagctg ctagcggcgg ccgtgccgcc
     2761 ggagcggctc aagttgttcc gttttccgac caacgacgcc tgggcacgcg accacggcgc
     2821 gatcttcgtg acgcggccga cggccgactc gccgcgtttg gccgtggatt tcgactacaa
     2881 cgcttggggc gggaagtacc cgccgttcga cctcgatcgg cagatcggtc gcctaatggc
     2941 cgaggcgctc cgcgtgccgc gctacgcgca gcccggcatc gtgctcgagg gcggctcgat
     3001 cgaggtcaac ggcgcaggcg cgctgctgac gaccgagcag tgcctgctga atccgaaccg
     3061 caacccgacg ctgacgcgcg ccgacctcga gcgtttgttg cgcgacagct tcggcgtgcg
     3121 cgagatcctc tggctcggcg agggcatcga gggcgacgac acggacggcc acatcgacga
     3181 cctcacccgg ttcgtgggcg ccacgaaggt cgtgaccgtc gtcgagtcga accgcgcgga
     3241 tcccaatcac gcgccgctcg cggcgaaccg gcggctgctc gacgcgctga cggtcggcgg
     3301 gcggccgctc gaggtcgcag agctaccgat gccggttccg cagtacctcg aggaccagcg
     3361 tttgccggcg agctacgcga atttctacct cgcgaacggc gccgtgctcg tcccggtctt
     3421 cggctgcccg gccgacgaca ccgcgtgcgg aatcttgggc gactgctttc cgggccgtcg
     3481 cgtggtgccg atcgactgcc gtgttctcat tgccggcttg ggcgctctgc actgcatgac
     3541 gcagcaggtg ccgattgaac cgatcgtcag ccgggaggtc atcggggttg tgggccgggg
     3601 ctctatttcc taatcttcac gctttcgacg ggcgcctggt cgcctaaagg ctattcgcga
     3661 tgggcgcaat ctcggatttc atccgccatc actatcggca tttcaacggg gccgcgctcg
     3721 tcgatgccgc ggatgcctac gtgaagcatc tcgacgcggg cgggcaaatg ctggtcacgc
     3781 tcgcgggcgc catgagcacg gccgagctcg gcctgtcgct cgcggagatg atccggcgcg
     3841 gcaaggtcca tgcgatctgc tgcacgggcg cgaacctcga agaggacgtc ttcaacctcg
     3901 tcgcgcacga tcactacgtc cgcattccga actaccgtta tctgtcgccg gagcaagaag
     3961 aggagctgct cgaccagcat ctgaaccgcg tgaccgacac ctgcatcccg gagcacgagg
     4021 cgattcgtcg catcgagaaa gccgtgctcg acgagtggca gcgggccgac aaagccggcc
     4081 agaagttttt cccgcacgag ttcatgtaca aaatcctgcg ctcgggcgtg ctcgaagagc
     4141 actatcaaat cgatccgcaa gacagctggc tcttggccgc ggcggagcgc gacctgccga
     4201 tgttcgtgcc tggctgggag gactcgacgc tcggcaacat tttcgccggc cactgcatcg
     4261 ccggcgacgt cgacaacgtc cacacggtca gaaccggcat cgagtacatg atggtgctcg
     4321 ccgattggta tcagacgacc tgccgcaaag gctcgatcgg cttcttccag atcggcggcg
     4381 gcatcgccgg cgactttccg atctgcgtcg tgccgatgct gcagcaggac ttgcagttgc
     4441 ccgacacgcc gcgctggggc tacttctgcc agatcagcga ctcgacgacg agctacggct
     4501 catactcggg tgccgtgccg aacgagaaga tcacctgggg caagctgtcg gcttcgaccc
     4561 cgaagttcat catcgagtcc gacgccacga tcgtcgcgcc gctgatgttc gctcacgtgc
     4621 tcggagagta gcgaagatgt cgacccagcc gcagaccaaa ccttcaaccg tcgaatggca
     4681 accgtcggac gcgtccgacc tctatcggat tccgagctgg ggcgacccgt tcttcttcgt
     4741 caatgacaag ggccacatgg ccgtgcgcgc gctcgacgag cacggcacga cgatggatgt
     4801 cgtcgacatc gtcaacgagc tgcgccgccg cggcgtgcag tttcccgtgc tgctgcgctt
     4861 ccaagacgtg ctgcgcgctc aggtgcggcg cgtcaacgag gcgtttcgca gcgcgatcgc
     4921 cgagtcgaac tacggcaacg tctaccgcgg catctatccg atcaaggtca accagctgca
     4981 cgaggtcgtc gacgagctgc tcgacgcggg ccggccctac ggcatgggcc tcgagtgcgg
     5041 ctcgaaggcc gagctgatcg cgtgcctgcc gcacatcgac ggtgagacgc tgctcgtctg
     5101 caacggcgtc aaggaccgca cgatgctgtc gctgatgatc gcgggccaga ggctcgggca
     5161 gaacatcgtg cccgtcatgg agaagtactc cgagttcggc gacgtcaaag gcctcgcgtt
     5221 ctcgacgggc tacatgtcgc agctcggtgt gcgcgtgcgg ctcgcgacgc gcggcgccgg
     5281 gcggtgggcc gagtcgtcgg gcacgaactc gaagttcggc ctgaacgtcg ccgagctcat
     5341 gcgcatggtc aacgagctcg aggcgagcgg ccagcagtcg aagctcgtcc tgctgcactg
     5401 ccacatcggc agccagatcg ccgacatcca agtgctcaag gaagcgacga aggaaatcgc
     5461 gcagatctac gcacagctcg tgaagcgcgg catcgcgctc aagtatctcg acctgggcgg
     5521 cggcctcggc gtgaactacg acaagggcca tctcgaggaa gacgccggca tcaactacgc
     5581 gctcgaggag tacgcgaacg ccgtcgtgtt cacggtcaag gaagtttgcg acgccaacga
     5641 ggtgccgacg ccgacgctcg tcacggagag cggccgcgcg ttgacggcgc accactcggt
     5701 gctgatcgtg cccgtgctcg gcgcgcacgc gaaggacgac ccgacccaag acctcacggt
     5761 gcccgagaac gcggccgagc cgctgcatgc gctcacgaag atcctcacgg cgctgccgaa
     5821 gacgcgcaag caatcggagc tgctcgaggc ctatcacgac atcaacgagc ggttggaaga
     5881 ggtgcgccag ctgttcacgc tcggctactt gccgctcgcg gagcgcgcga tcgccgagag
     5941 cctttactgg cgcatctgca cgggcctgct gaaggcgctg aagcgcggca agcacgacgg
     6001 gtcgcgcccc gagatcatcg agctcgagga caagctgacc gagctctacc tctgcgactt
     6061 ctcggtgttc cagtcgatgc tcgaccactg ggcgatcggc cagccgttcc cgatcgtgcc
     6121 gatcgaccgg ctcgacgagc agcccgagaa gcgcgcgatc ctcgtcgatc tcacttgcga
     6181 ctcggacggc aaggtcacgc attacgtgtc gacgcgcgac aaccgtttcc tgcccgtgca
     6241 cccaaccgtg aacggcacgc cgtattacct cgggt
//
LOCUS       DATLEE010000381         2861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1173031, whole genome shotgun sequence.
ACCESSION   DATLEE010000381 DATLEE010000000
VERSION     DATLEE010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173031"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..830
                     /locus_tag="VM692_08175"
     CDS             36..830
                     /locus_tag="VM692_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUQ52185.1"
                     /translation="MERNFIRWRGQRICVSEVGAGKPLFLVPGIGCSADMWQPFMPYF
                     PNRRIISFDAPGTGRSSTPFFPVPVTSLAELAVTVLDNRGVDCADVIGFSYGGAVAQQ
                     LAYGYPERVCRLVLAATNFGWGSMLGSPQALAVLATPLRFYSASYFERVAPDVYGGAT
                     GRDPAKRQGASGARRRLPPTQYGYAMQLLGGIGWSSWGFLPDIPHETLVVCGDDDPLV
                     PVANARMIADRIPRAMLSVYERAGHLLLWDEPERVAPQIGQFVGWA"
     gene            887..1825
                     /locus_tag="VM692_08180"
     CDS             887..1825
                     /locus_tag="VM692_08180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HUQ52186.1"
                     /translation="MRGRFVSIALGCAAVAAAAVTFAQTDNWYPSRWGAADQRGAANR
                     ITPAKVMEAKNLITSGNVYQLGHVYEATMPLFGTRHFSLRIPQTYGPMGTNRMMYHDE
                     LVSSEIGQVGTQFDGLGHVGIGDLFYNGNNRADFSRPEGFTKLGIENVGALVTRGVLV
                     DVAALKGVPRLEGGYEITRADLTAALERERVQVRSGDMVLLHTGWGSLWGVDNAAFGA
                     KAPGIGLDAAQYLVDREVVLVGADTWSMEVVPNPNPDLQFPVHQVLLARNGIYIFENL
                     VTDELARDRAYEFAFFFAPLRMKGATGSPGNPLAIR"
     gene            complement(2103..2402)
                     /locus_tag="VM692_08185"
     CDS             complement(2103..2402)
                     /locus_tag="VM692_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUQ52187.1"
                     /translation="MPLYIVQFEDKPNMGELRDKLLKSHFEFLDRVKDRVLVPGSMRE
                     VPSDKPLGGLWIIEAKDEAEVRDIFKDDPFWTGGLRAQVRINRWQKAFPDRKVAI"
     gene            complement(2469..>2861)
                     /locus_tag="VM692_08190"
     CDS             complement(2469..>2861)
                     /locus_tag="VM692_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52188.1"
                     /translation="LRNIEHNHVCHAHIVILHMEILRMPRQDRAARAWVEELMPDVHA
                     VHARFGFMETPDVAEALRGARQRGLRFDVDCTYFLGWHLVRARPRSGLSGLKSQVFAY
                     LQRRSAQAAEFFRMPTKRVVVLATEIDI"
BASE COUNT          435 a          922 c          964 g          540 t
ORIGIN      
        1 caccgtggaa ttcctgggct cggcggaggg ccgacgtgga gcgtaatttc atccggtggc
       61 gcggtcagcg gatctgcgtc tctgaagtgg gggcaggcaa gccgctgttt ctggttcccg
      121 gcatcggctg cagcgccgac atgtggcagc cattcatgcc gtacttcccc aatcgccgca
      181 tcatcagctt cgacgcgccc ggcacgggcc gctcgagcac gccgttcttc cccgtgcccg
      241 tcacgtcgtt ggccgagctc gccgtcaccg tgctcgacaa tcgcggcgtc gactgtgccg
      301 acgtcatcgg tttttcgtac ggcggcgccg ttgcccaaca gctcgcttac ggctatcccg
      361 agcgcgtctg ccgcctcgtg ttggcggcga ccaattttgg ctggggctcc atgttgggct
      421 cgccgcaagc gttggctgtg ctcgcgaccc cgctgcggtt ctactcggcg agttacttcg
      481 agcgcgtagc gcccgacgtc tacggcggcg ccacggggcg cgaccccgcg aagcgccagg
      541 gcgcgagcgg cgcacgccgg cgcttgccgc cgacgcaata cggctacgcg atgcagctgc
      601 tgggcggtat cggctggtcg agctggggct ttttgccgga cattccgcac gagacgctcg
      661 tcgtctgcgg cgacgatgat ccgctcgtgc cggtcgcgaa tgccagaatg atcgcggatc
      721 ggattccgcg cgccatgctc tcggtctacg agcgcgcagg ccacttgctg ctgtgggacg
      781 agccggagcg cgtggcaccg cagatcggac agttcgtcgg ttgggcttga ttgcactcga
      841 cgtagatcga gcggctcagt ttcgcgcttg cacaggagga gtcacgatgc gtggccgttt
      901 tgtttccatc gccttgggtt gtgccgccgt cgcggcggct gccgtcactt tcgcccaaac
      961 ggacaactgg tacccgtcgc gttggggcgc tgcggatcag cgcggggctg ccaatcgcat
     1021 cacgccggcg aaggtcatgg aggcgaagaa cctcatcacg agcggcaacg tctaccagct
     1081 cggtcacgtg tacgaagcta cgatgccgct gttcggcact cgacatttca gcctgcgcat
     1141 tccgcagact tacggcccga tgggcacgaa tcgaatgatg taccacgacg agctcgtcag
     1201 ctccgagatc ggccaggttg gcacgcagtt cgacggtctc ggccacgtcg gcatcggcga
     1261 tctcttctac aacggcaata accgcgccga cttctcgcgg cccgaagggt tcacgaagct
     1321 cggcatcgaa aacgtcggcg cgctcgtgac gcgcggcgtg ctcgtcgacg tcgcggcgct
     1381 caagggcgtc ccgcggctcg agggcggcta cgagatcaca cgtgccgatc taacggcagc
     1441 gctcgagcgc gagcgcgtgc aggtccgttc cggagacatg gtgctgctgc acacgggttg
     1501 gggttcgctg tggggcgtgg acaacgccgc attcggcgcc aaagcgcccg gcatcggctt
     1561 ggacgctgcg caatatctcg tcgaccgcga ggtcgtgctc gtcggcgccg atacgtggtc
     1621 gatggaagtc gtgccgaacc cgaaccccga tctgcagttc cccgtgcacc aggtactgtt
     1681 ggcgcgcaac ggcatctaca tcttcgagaa tctcgtcacg gacgagctgg cgcgcgaccg
     1741 ggcgtacgag ttcgcgttct tcttcgcgcc gctgcgcatg aaaggcgcga cgggatcgcc
     1801 ggggaatccg ctcgcgattc ggtgaatccg ttgcttggcg gtggtgccac aacgggcccg
     1861 acggagtgat cgcgcttcgc gctctagccc cgatgtattg gatcgctctt gctctgcgac
     1921 gcgggccggg cgcgtcttcg aagctttgcc tcgccgcgtg tagcggctcg acctctccgt
     1981 gacggcggct gagagttgtt ggcccgcttt cgtgcggggc taaccgcgcg aagcacgacc
     2041 actccatcgg gcccgctgct ccagcggatc ggacggattc acgcagcgcg ggccggttgc
     2101 gcttagatcg cgactttgcg gtcggggaag gctttttgcc agcggttgat gcgcacctgc
     2161 gcgcgtagcc cgccggtcca aaacgggtcg tccttgaaaa tgtcgcgcac ctcggcttcg
     2221 tctttcgctt cgatgatcca gagcccgccg agcggcttgt cgctcggcac ctcgcgcatc
     2281 gagcccggca cgagcacgcg gtctttgacg cgatcgagga actcgaagtg cgacttcaaa
     2341 agcttgtcgc gcagctcgcc catgttgggc ttgtcctcga attgcacgat gtagagcggc
     2401 atggcggtcc ctcgtcgtta gcggaagctc gaagtgcggt tagcctagcg tgtcggcgcg
     2461 cgccgcggct agatgtcgat ttcggtggcc agcacgacga cgcgcttcgt cggcatgcgg
     2521 aagaactcgg cggcctgcgc gctgcgccgc tgtagatagg cgaagacttg cgacttcagt
     2581 cccgagagcc cggagcgcgg ccgtgcgcgc acgaggtgcc aacccaagaa gtacgtgcaa
     2641 tcgacgtcga accggaggcc tcgttggcga gcgccgcgta gcgcctcggc gacatcgggc
     2701 gtctccatga agccgaagcg cgcgtgcacg gcgtgcacgt cgggcatcag ctcttcgacc
     2761 caggcacgcg cggcgcggtc ctggcgcggc atgcgcagga tctccatgtg cagtatcacg
     2821 atatgcgcgt ggcacacgtg gttgtgctcg atgttgcgca g
//
LOCUS       DATLEE010000382         2365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4133844, whole genome shotgun sequence.
ACCESSION   DATLEE010000382 DATLEE010000000
VERSION     DATLEE010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4133844"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1012)
                     /locus_tag="VM692_08195"
     CDS             complement(134..1012)
                     /locus_tag="VM692_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ52189.1"
                     /translation="MSSIPVCMAPDPNTRAPKYRPPPGACDAHCHIFGPGDKYPYAAD
                     RNYTPPDAPLERFIELQKTLGLTRAVLVNASCHGVDNTVILDAIAQSGGRYRGVANVD
                     ESFTDRDFAKLHDGGIRGIRFNFVQHLGGTPDMAEFKRLVARAAAFGWHVVLHFDAKD
                     LLEFDSLLRTLPVPFIIDHMGRVPTKDGLEQEPFKILLNVARMDNCWVKICGAERISS
                     QGPPFTDAVPFAQAILKVAPERALWGTDWPHPNIKKHMPNDGDLVDLIPKFMPDAALQ
                     RQVLVDNPQRLYGFTG"
     gene            complement(1005..1949)
                     /locus_tag="VM692_08200"
     CDS             complement(1005..1949)
                     /locus_tag="VM692_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HUQ52190.1"
                     /translation="MKIVMVGQGAFGRKHLDGLKNIRDVEVVSLVGGSKESTEAVAKQ
                     YGIAHATTNLAEALALPGVDAAILTSPTQVHAAQAEQVMKAGKHVEIEIPIADSYADA
                     KRIHTVQKETGKIAMAGHTRRFNPSHLWVANRIKKGELKVQQLVVQTYFFRRKNINLE
                     GKPRSWTDHLLWHHACHTVDLFHYVTGEVPSVVHALQGPLHPDLKIAMDMGVTMRTPS
                     GVVCNLSLSFNNDGPQGTFFRYICDNGTYIARYDDLFDGKENKIDLSQVAVSMNGIEL
                     QDREFVAAIREKREPFSSVAQVLPAMETLDRLEKSLNV"
     gene            2070..>2365
                     /locus_tag="VM692_08205"
     CDS             2070..>2365
                     /locus_tag="VM692_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUQ52191.1"
                     /translation="MTLRTDFSLLIDGELEQSAQHLDVINPALGAVFARCPTAGSAEL
                     DQAVAAARSAFAAWRARSFAERASLVKQLSAQLRAKQNELAELQRRQRRVRRRF"
BASE COUNT          398 a          762 c          755 g          450 t
ORIGIN      
        1 tcgccgccgc cgttaccatg gaaccccgcc gcatcgccgt catgtccgct cccgctgaat
       61 ggcgagccga tcatgagccg cgcgcgacga gcgcacaagg cgctgcgacg ttcggcccac
      121 ctggcccgtg aggtcagccc gtgaaaccgt agagccgctg tgggttgtcg acgagcactt
      181 ggcgctgcaa cgcagcatcg ggcatgaact tcgggatcag gtcgacgagg tcgccgtcgt
      241 tcggcatgtg cttcttgatg ttcggatgcg gccagtcggt gccccacagc gcgcgctccg
      301 gcgcgacttt caagatcgcc tgcgcgaacg gcacggcatc cgtgaacggc gggccttgcg
      361 acgagatgcg ctcggcgccg cagatcttga cccagcaatt gtccatgcgc gcgacgttca
      421 acaggatctt gaacggctct tgctcgaggc catccttcgt cggcacgcgc cccatgtgat
      481 cgatgatgaa cggcacgggc agcgtgcgca gcagtgagtc gaactcgagc aaatccttgg
      541 cgtcgaagtg cagcacgacg tgccaaccaa acgctgccgc acgcgcgacg agccgcttga
      601 actcggccat atcgggcgtg ccgcccaagt gctgcacgaa gttgaagcga atgccgcgga
      661 tgccgccgtc gtgcagcttg gcgaaatctc gatccgtaaa gctctcatcg acgttcgcga
      721 caccgcgata gcggccgccg ctctgcgcga tggcgtccaa gatcaccgtg ttgtcgacgc
      781 cgtggcagct cgcgttgacg agcactgcac gtgtcagacc caacgtcttc tgcagctcga
      841 tgaagcgctc gagcggggca tcgggcggcg tgtagttgcg atccgctgcg tacgggtatt
      901 tgtcgccggg gccgaagatg tggcaatgcg cgtcgcaagc gcccggcggc ggacggtact
      961 tcggcgcgcg cgtgttcggg tccggcgcca tgcagaccgg gatgctagac attgaggctc
     1021 ttctccagcc gatcgagcgt ttccatcgcg ggcagcactt gcgcgacgct cgagaacggc
     1081 tcccgcttct cgcggatcgc cgccacgaac tcacggtcct ggagctcgat gccgttcatc
     1141 gacaccgcga cctgcgacag gtcgatcttg ttctccttgc cgtcgaagag atcgtcgtag
     1201 cgtgcgatgt acgtgccgtt gtcgcagatg taacggaaga aggtaccttg cggcccatcg
     1261 ttgttgaacg acagggacag attgcagacg acgcccgacg gcgtgcgcat cgttacgccc
     1321 atgtccatcg cgatcttgag gtcggggtgc agcggcccct gcagcgcgtg tacgaccgac
     1381 ggcacttcgc ccgtcacgta gtggaacaag tcgacggtgt gacacgcgtg gtgccacagc
     1441 aagtgatcgg tccagctgcg cggcttgccc tccagattga tgttcttgcg gcgaaagaaa
     1501 taggtttgca cgacgagctg ctggaccttc agctcgcctt tcttgatgcg gttcgcgacc
     1561 cagagatgcg acggattgaa ccgccgagtg tgccccgcca tcgcaatctt gccggtttcc
     1621 ttctgcacgg tgtgaatgcg cttcgcatcg gcgtacgaat cagcgatcgg gatctcgatc
     1681 tcgacgtgct taccggcctt catgacttgc tcggcttgcg cggcgtgcac ttgcgtcggc
     1741 gacgtcaaga tcgcggcgtc cacgcccggc aacgcgagcg cctcggcgag attcgtcgtc
     1801 gcgtgcgcga tgccgtattg cttcgcgacc gcttccgtcg actccttgct gccgccgacg
     1861 aggctgacga cttcgacgtc gcgaatgttc ttcaggccat cgaggtgttt gcgcccaaac
     1921 gcgccctgcc cgaccatgac gatcttcatc atcgctctcc gtttgttggg cgggttccga
     1981 atcggccgcc cgcggaccgc gcggctcgct cgaaaattcg cgccggttca ctcgtgtatt
     2041 atcgcgaaat ggcacagcgc gcacaaagcg tgacgttgag aacggatttc tctctgctga
     2101 tcgacggtga gctcgagcag agcgcgcagc atctcgacgt catcaatccc gcgctcggtg
     2161 ccgtgttcgc gcgctgccca accgcgggaa gcgcggagct cgaccaggcc gtcgcggcgg
     2221 cgcgcagcgc atttgccgca tggcgcgcgc gctcgttcgc cgagcgggcg tcgctcgtca
     2281 agcaactttc ggcacagctg cgcgccaagc agaacgagct cgccgagctt cagcgtcgtc
     2341 agcggcgtgt acggcgaagg tttga
//
LOCUS       DATLEE010000383         2906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2665443, whole genome shotgun sequence.
ACCESSION   DATLEE010000383 DATLEE010000000
VERSION     DATLEE010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2665443"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VM692_08210"
     CDS             complement(<1..561)
                     /locus_tag="VM692_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HUQ52192.1"
                     /translation="MKALDPAAAADATLCADDIEACLRVLRAIEADRSHLTRLTREQR
                     RELLTLAGLVAKPERHDLVRMAKAFRRAEREAARAHDRMLIDQAALRVQRRSPVYTPL
                     RLEPPAPEQAASRAELNNELSCYVCKQPFAKVHRYYDSLCEPCGDFNYAKREQTADLA
                     GHYALVTGARVKIGYQAALKLLRAGAN"
     gene            complement(603..2081)
                     /locus_tag="VM692_08215"
     CDS             complement(603..2081)
                     /locus_tag="VM692_08215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUQ52193.1"
                     /translation="MTTPPALAPDAIPGPRRRRYALLINPFYRKDPHASFGKHVLTPT
                     LALTATAAATPPHWELRYWDENLLQGPPPLDPFPEVVGISVHLTFAKRAYELARWYRQ
                     WGAKVVLGGLHVLTAPDEAAEFADALAIGEGVQIWPQILADVEAGKLERVYRGDFAKP
                     YRDDPAPRRDLLPRHGFLTTSSVIATRGCHNRCGFCYLSTKGLHMPYRLRDVEQVVAE
                     LKADGQPYAVFVDNNLGSRPDYLRELCLALRPLEIIWSAALTIDVTDDPSLVREMALA
                     GCTGVFIGFESLNAGNLEAARKRTPVPEDYARRVRILHDHGIQVNGSFVLGFDHDEAD
                     VFEKTVAWIEENRLECATFHILTPYPGTPLFNELESERRVVHRDWRLYDTAHVVFEPK
                     RMSAEQLFNGYQWCYRRLFSHRSIWRRRPRDWRAVLPYLAMSYLYKRSNLIWQLLIRF
                     RLTAAIWRPLIELSRRRHLRFRRRLERAQSGGETRGVVSAGV"
     gene            2469..>2906
                     /locus_tag="VM692_08220"
     CDS             2469..>2906
                     /locus_tag="VM692_08220"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HUQ52194.1"
                     /translation="MSIRNPSLRTHALRGANLRLSVLAVAIAAPPLLVTAQQSTEEII
                     VTGSRVSRSGFDSSQPLTTIDSQQIENLGLTNVGDVVRTLPQNTPFFTETNVGIGNFN
                     VGAQLANLRGLNPFFGTRTLTLVDTKRVVPTTEGGAVDLTLIPS"
BASE COUNT          489 a          965 c          959 g          493 t
ORIGIN      
        1 gttggcgccg gcgcgcagca gcttcaaggc ggcttgatag ccgatcttga cgcgcgcgcc
       61 ggtgacgagc gcgtagtgac cggccaggtc cgccgtctgc tcgcgcttcg cgtagttgaa
      121 gtcgccgcac ggctcgcaca gcgaatcgta gtagcggtga accttcgcga agggctgctt
      181 gcacacgtaa cacgagagct cgttgttgag ctcggctcgt gaagctgctt gctcgggcgc
      241 cggcggctcg agccgcagcg gcgtgtaaac aggcgaacgc ctctgtacgc gcagcgcagc
      301 ctggtcgatc aacattcgat cgtgcgccct cgccgcttcg cgctcggccc ggcggaacgc
      361 cttcgccatg cgcacgagat cgtggcgttc gggttttgcg acgaggcctg cgagcgtcag
      421 cagctcgcgg cgctgctcgc gagtcaggcg cgtcaaatgc gaccgatcgg cttcgatcgc
      481 acgcaggacg cggaggcacg cttcgatatc gtccgcgcag agagtcgcgt ccgcggccgc
      541 agccggatca agagctttca tggcgcgcat tgtatgccgc cacgcgcggc gcggcgtcga
      601 gctcagacgc ccgcgctgac gacgccgcgc gtctccccgc cgctctgcgc acgctcgagc
      661 cggcgccgga aacgcaggtg acgccgccgg ctgagctcga tcaacggccg ccaaatcgcg
      721 gccgtcaagc gaaagcggat cagcagctgc cagatgaggt tcgagcgctt gtacagatac
      781 gacatcgcga ggtacggcaa caccgcgcgc cagtcgcgcg ggcggcgccg ccagatcgag
      841 cgatgcgaga acaggcgccg gtaacaccac tgatagccgt tgaacagctg ctccgcgctc
      901 atgcgcttgg gctcgaagac gacgtgggcc gtgtcgtaga ggcgccagtc gcgatgcacg
      961 acgcggcgct cgctttcgag ctcgttgaag agcggcgtcc cggggtacgg cgtaaggatg
     1021 tggaacgtcg cgcactcgag gcggttctct tcgatccagg cgaccgtctt ctcgaataca
     1081 tcggcctcgt catgatcgaa cccgagcacg aagctgccgt tcacctggat accgtggtcg
     1141 tgcaggatcc tcacgcggcg cgcgtaatct tccggcacgg gggtccgctt gcgcgccgct
     1201 tcgagattgc ccgcgttcaa cgactcgaaa ccgatgaaga cgcccgtgca gccggcgagc
     1261 gccatctcgc ggacgagcga cggatcgtcg gtgacgtcga tcgtcaatgc ggcgctccag
     1321 atgatctcga gcggccgcaa ggcaaggcac agctcgcgaa ggtagtccgg gcgcgagccg
     1381 agattgttgt cgacgaagac ggcgtacggc tggccgtcgg ctttgagctc tgcgacgacc
     1441 tgctcgacgt cgcgcagccg atacggcatg tgcagccctt tcgtcgacag gtagcaaaag
     1501 ccgcagcggt tgtggcaacc gcgagtcgcg atgacgctcg acgtcgtcag aaacccgtgg
     1561 cgcggcaaca ggtcgcgccg cggcgccgga tcgtcgcgat acggcttcgc gaaatcgccg
     1621 cgatagacgc gctcgagctt gccggcttca acgtcggcga ggatttgcgg ccaaatttgc
     1681 acgccttcac cgatcgcgag tgcgtcggcg aactcggcag cttcgtcggg tgccgtcaac
     1741 acgtgcaggc cgccgagcac gaccttggcg ccccactgtc gataccaacg cgcgagctcg
     1801 tacgcccgct ttgcgaacgt taggtgcacg ctgatgccga caacctcggg aaacggatcg
     1861 agcggcggcg gcccctgcag caggttttcg tcccagtaac gcagctccca gtgcggcggc
     1921 gttgccgccg ccgtggccgt cagcgcaagg gtcggcgtca gcacgtgctt gccgaagctc
     1981 gcgtgcgggt ccttgcgata gaacggattg atcaacagcg cgtagcgccg gcgccgcggg
     2041 cccggaatcg cgtcgggcgc gagcgccggt ggcgtcgtca tggatttcgg caaccatggc
     2101 ttcggttggc gagctgttac ggcgttcacg acacctccca aagacgggct aacgtcggcc
     2161 gaagatagcc tgctacgagg cattgtcaag tactttgcca tgcaaagctg acgatgcgca
     2221 ccgcccggct cggtggcttt gcgtcgcggc gcccgccctg cctgccgtgc agccgatacc
     2281 gccagagcat tccgcccgct agcggacggt ccgatttcgg gcatacttgg ccggggctca
     2341 gttccgaccg actcgcgtga ccgtggctct cgggtgctca gccccacttg ccgtcggcct
     2401 cgccggtgct cgcaagacgc cgtcgaaggc cgttcgaacg atcagaactt ctttttgggg
     2461 gctagcctat gtcgatccgt aacccgtcgc tgcgaaccca tgcgttgcgc ggcgccaacc
     2521 tgcgtttgtc cgttcttgcc gtggccatag cggctccgcc gctgcttgtg acggcccagc
     2581 aaagcactga ggagatcata gtcaccggct cgcgcgtcag ccgcagcggc ttcgactcgt
     2641 cgcagccgtt gacgacgatc gactcacagc agatcgagaa tctgggcctg accaacgtcg
     2701 gcgacgtcgt gcggacgctg ccgcagaata cgccgttctt caccgagacg aacgtcggta
     2761 tcggtaactt caacgtcggc gcacagctcg cgaacctgcg cggcctgaac cccttcttcg
     2821 gcacgcgcac cctgacgctc gtcgacacga agcgtgtcgt tccgacgacg gagggcggcg
     2881 ccgtcgacct gacgctaatt ccctcg
//
LOCUS       DATLEE010000384         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4137211, whole genome shotgun sequence.
ACCESSION   DATLEE010000384 DATLEE010000000
VERSION     DATLEE010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4137211"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VM692_08225"
     CDS             complement(<1..511)
                     /locus_tag="VM692_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009907306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HUQ52195.1"
                     /translation="MTVLESPAAERNKQPIATVLARVLPTTGLVLEVASGTGQHAEHF
                     ARALPSTTWQPTEAETGLLAALAARVQRAALPNLRVPLAFDVHDELAPVDSAAAVFCA
                     NMIHIAPSSAFPALLAHAERLLAAGAPLVLYGPFKRGGEHTAPSNAAFDADLRRRNPE
                     WGIRDLDDVV"
     gene            complement(525..1547)
                     /locus_tag="VM692_08230"
     CDS             complement(525..1547)
                     /locus_tag="VM692_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52196.1"
                     /translation="MHRYDGRSVTLSALSFVVVAGLATAQSATAPSRYQTPRTSWGDP
                     DLAGIYSNDDETGTPLERPSEFEGLTLKTVTPEKIAEIVAERDRRFKAQFDSEAWRRS
                     ISPPPHLIFDTFDRKNKRPWLITDPEDGKIPPRTAEADARPRTGGVSTNQNSRGPFLS
                     YTDMGLYDRCITRGIPNSMMPAGYGSRYEIVQAPDSVAIRYEMIHEARVIPLDRRPPS
                     GTTKGRPHIAPGLKQSLGDARGWWDGDTLVVETTNFRGETAPQRSSENVKMIERFERT
                     SADSVEWTVTFEDATTWERPWTFSMPLAQVDHTQQIYEYACHEGNYAMRNILSGARAA
                     EAAAAK"
     gene            1774..>2080
                     /locus_tag="VM692_08235"
     CDS             1774..>2080
                     /locus_tag="VM692_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52197.1"
                     /translation="MITTNRFSFLSRGAALSLVAIVSAGLLAACTQGPQAPAAPQGPQ
                     LPRLGGKPDFNGVWQAIGTAYWNLEDHSASGLADFWQLGAIAAVPAGQSVVEGGTIPY
                     "
BASE COUNT          328 a          712 c          679 g          361 t
ORIGIN      
        1 cgacgacgtc gtcgagatcg cgaatgcccc attcgggatt tcgccggcgc aagtcggcgt
       61 cgaacgccgc gttgctcggc gccgtgtgct cgccgccgcg tttgaagggg ccgtacagca
      121 cgagcggcgc gccggccgcg agcaagcgct cggcgtgagc caacagcgcg gggaacgccg
      181 acgacggcgc gatgtgaatc atgttggcgc agaacacggc cgccgcggag tcgacgggcg
      241 cgagctcgtc atgaacgtcg aacgcgagcg gtacccgcag attcggcagc gcggcacgtt
      301 gcacgcgcgc tgcgagcgct gccagcaagc cggtttcggc ttccgtcggc tgccacgtcg
      361 tgctcggcaa agcgcgcgcg aagtgctcgg catgctgccc cgttccgctg gcgacctcga
      421 gcacgaggcc cgtggtagga agcacgcgcg cgagcaccgt cgcgatcggc tgcttgttgc
      481 gctcggcggc cgggctctcg aggaccgtca cgtgcagcgg ctgactattt cgcggccgcg
      541 gcctcggcgg cccgcgcccc gctcaggatg ttgcgcatcg cgtagttgcc ttcgtgacac
      601 gcgtactcgt agatctgctg cgtgtgatcg acctgcgcca acggcatcga gaacgtccac
      661 gggcgttccc atgtcgtcgc gtcctcgaac gtcacggtcc actcgacgct atcggccgac
      721 gtgcgctcga agcgctcgat catcttgacg ttctcgctcg aacgctgcgg cgcggtctcg
      781 ccgcgaaagt tcgtcgtctc gacgacgagc gtgtcgccgt cccaccagcc gcgcgcatcg
      841 cccaaggatt gcttcaaccc cggcgcgatg tgcggccgcc ccttggtagt ccccgaaggg
      901 gggcggcgat cgagcggaat cacgcgcgcc tcgtggatca tctcgtaacg gatcgcgacg
      961 ctgtcgggcg cctgcacgat ttcgtaacgg ctgccgtagc cggccggcat catcgagttc
     1021 gggatgccgc gcgtgatgca gcgatcgtag agacccatgt ccgtgtagct caagaacggg
     1081 ccgcgcgagt tctgattcgt gctgacgccg cccgtccgcg gccgcgcatc cgcctcggcc
     1141 gtccgcggcg ggatcttgcc gtcctcggga tccgtgatga gccacggtct cttgttcttg
     1201 cgatcgaacg tatcgaagat caagtgcggc ggcggcgaga tgcttctgcg ccaggcttcg
     1261 ctgtcgaact gcgctttgaa gcgtcgatcg cgctcggcga cgatctcggc aatcttctcg
     1321 ggcgtgacgg tcttcagcgt caggccttcg aactcgcttg gccgctccag cggcgtgccc
     1381 gtctcgtcgt cgttcgaata gatacccgcg aggtcggggt cgccccatga agttcgcggc
     1441 gtctgatagc gactcggcgc cgtagcgctt tgcgccgtcg caagccccgc taccacgaca
     1501 aaacttaagg cactcaacgt cactgaacga ccgtcgtaac ggtgcatggc atcctccccc
     1561 cgaagcccga gcgcgcgcct gaaagccaag cccgacgcgg ctttgacatc cgcagcgcgt
     1621 tcgcatagaa cgactgccag aaacacgaac tatgcccgat tctacgtgcg gcctagcgag
     1681 ggagctacgc cgacacggca cgggcgacgc gacgacgacg ctggcttttt acgactcggc
     1741 ctaccaataa cttctgaagg gcggtgctaa ccaatgatca cgaccaatcg cttctcgttc
     1801 ctgtcgcgcg gcgcggcgtt gtcgctcgtg gcgatcgtca gcgccggcct gctcgccgct
     1861 tgcacgcaag gcccgcaagc gcccgccgcg ccgcagggac cgcaactgcc gcgcctcggc
     1921 ggcaaaccgg atttcaacgg cgtttggcaa gcgatcggca ccgcttactg gaacctcgag
     1981 gatcactcgg cttcgggcct agcggatttc tggcagctcg gcgccattgc ggcggtcccg
     2041 gccggccaaa gcgtcgtcga gggcggcacg atcccgtact
//
LOCUS       DATLEE010000385         6516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2668795, whole genome shotgun sequence.
ACCESSION   DATLEE010000385 DATLEE010000000
VERSION     DATLEE010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2668795"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VM692_08240"
     CDS             complement(<1..183)
                     /locus_tag="VM692_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUQ52198.1"
                     /translation="MFPIRDDNPHFLTPVVTYGLIAANVLVWVLIQGFGSEPTLSSSI
                     CRLGLIPGEFLQRAAAG"
     gene            complement(284..637)
                     /locus_tag="VM692_08245"
     CDS             complement(284..637)
                     /locus_tag="VM692_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52199.1"
                     /translation="MKAAERPIGSLVVDGEPANHALYEEYVRGFDEIPVLDDAVRIPP
                     RRSPSPSFVHGERRSLRKEERDDGYSVREATLAEIESLYGEYLAPAAARPHRKQARAK
                     KKSSGKRRRKSSAAA"
     gene            complement(733..1662)
                     /locus_tag="VM692_08250"
     CDS             complement(733..1662)
                     /locus_tag="VM692_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HUQ52200.1"
                     /translation="MVAELADVRLSTAKRRAPRVGLVLGSGAARGWAHIGVIRALEKA
                     GVRPHLVCGTSVGALVGAAYASGDFDRFCAGTAGMRRRDVVSFMDVAMSGGLLKGERF
                     MAYLSRAWQDRPIEELAIPFAAVATSLESGAEVWLRTGSTKNAVRASLAMPGLFTPVM
                     SSDGILVDGALVNPVPVSLARAMGADIVIAVDLSAGIVGRSLVVEEAHVSRNTFVGEW
                     MRRGFGRLWGENSPRLPSVLNVVATSLDVMQVRITRSRMAGEPPDLMVAPRLADIGLL
                     DFHRADEAIREGERAMTAALGSLGVLGIQTAVP"
     gene            complement(1761..2216)
                     /locus_tag="VM692_08255"
     CDS             complement(1761..2216)
                     /locus_tag="VM692_08255"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HUQ52201.1"
                     /translation="MSAPPILTELKLSADMKKAVDTAFESLKPIVVSYVDESGAPQLS
                     FRGSTQAYSDTQLAIWVRNPEGRILESITKNPAMALLYGNFEPTGRAFMIFRGRARID
                     SSESGRRHIYERAHAFERDKDKDRKGIALIIDLDSVEGFFGGAMLKMRR"
     gene            2295..2843
                     /locus_tag="VM692_08260"
     CDS             2295..2843
                     /locus_tag="VM692_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ52202.1"
                     /translation="MCAFGQREAPILAQGFATLPRSHGVVDRRRLGWHVTCLFQRRKS
                     SSRESRATSVQGGTMIAHTGLAVREFAAAKKFYERALAPLGYSAKMEVGESAGFNDGK
                     NTDFWIGHSNAVEPGHVAFEAKSKREVDAFYRAALAAGGRDNGEPGYRPQYWPGYYAA
                     FVYDLDGHNVEAVWYDYAASNE"
     gene            2870..3355
                     /locus_tag="VM692_08265"
     CDS             2870..3355
                     /locus_tag="VM692_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HUQ52203.1"
                     /translation="MEESEHLCDLLKGFDTAMLVTRARDGYLHARPMAVAELRADADA
                     YFVTSIDSPKIAEIDADADVLLTFQSERQYAAVYGRVNVVQDRQLIERLWKEEWKVWF
                     PQGKSDPSIALLRFDAERGEFWDNAGTRGVEYAFEAAKAYVRGEKPHVDSDQHGRVRL
                     S"
     gene            complement(3357..4427)
                     /locus_tag="VM692_08270"
     CDS             complement(3357..4427)
                     /locus_tag="VM692_08270"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUQ52204.1"
                     /translation="MKVLVTGATGFAGKHLARALSASGHAVRALVRPGRRGRRLAESG
                     IEIFEGQLTNAADVREAANGCERIYHLAAAFRNVKHSDQHYWDVNVGGTMNVLAAARA
                     QGCERVVHCSTGGVHGHIEQPPAAETYRFRPGDVYQRTKLEAELAVAEASNNGLPISI
                     VRPGAIYGEGDMRFLRLFRAIQRQRFAMVGSGETRLHMVHVDDLVDGIVLAGSRPEAR
                     GETFLIAGPEAPTLNEIAATVADAVGAARPWLRVPVWPVYAAASLCEMVCVRLRVEPP
                     LHRRRVGFFTHHREFDITKARQLIGYEPRVKVAEGVRRTAAWYESRGLLTPLRSALAL
                     AQASLTALALDWSPAYTWVALV"
     gene            complement(4440..5654)
                     /locus_tag="VM692_08275"
     CDS             complement(4440..5654)
                     /locus_tag="VM692_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52205.1"
                     /translation="MRYAPSPTLRSMAVYGASGVAFAGANLLLARVLSTDQYALVTLL
                     VALSTLGYHLAPAGLDAVVIRGRVDAGPALLKRVAVTATSVASACCAVGIAAYGLSPA
                     TAALLLGATLAGGLMLAAAAKFQSEQRFTVSLALLNSPNLLLMLGALATLTVEAKTAT
                     LPFAILAVGLALMAGLSWALVLRERRGKTSLATAIPWGEAFALAGVAAAGMLLIQLER
                     LLIPYVLPLADLALFGVLGAIAGSLFRLLQMGVGFTLLPRLRSAPTVLERRQLIAREL
                     RLAVVISAAGAIAILVLTPLIERWLLAGKYHLPLGLVVAVLFSGIAKIAHAFAKAAAT
                     ALATQRELALVNAAGWFSVALAVGSAVVAARWGLTGVIYGVGVGWLAWAGLSFALVVR
                     HLRVPAGVPAET"
     gene            complement(5904..6377)
                     /locus_tag="VM692_08280"
     CDS             complement(5904..6377)
                     /locus_tag="VM692_08280"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52206.1"
                     /translation="MKSIVKLLPAVSAAALLTMAAAEAHHSFAMFDREQEIVKTGTVV
                     RWAFNNPHSWLYLNVKNADGTDTLWSFEGSAPPALIQRGITGATFEPGDTVQFMYCPL
                     RDGRPGGAIGWAKLEDGQYINPADGGCAGNEQNVNRWKDWLAKGFTSKADAEKAQ"
BASE COUNT         1142 a         2137 c         2178 g         1059 t
ORIGIN      
        1 accggctgcc gccctttgca ggaactcgcc ggggatcaga ccgaggcggc agatcgagct
       61 cgatagcgtc ggctcggagc caaaaccctg gatcagcacc cagaccagga cgttggctgc
      121 gatcaagccg tacgtgacga ccggcgtcag gaagtgcggg ttatcgtcgc ggatcggaaa
      181 catcggtcag ctctccatcg agcggacgta gcacgctgca tgtctcgtgc ctgcatcatg
      241 gcggcacgag cgacggagag aaaggcggcg tactgtgacg agcctaggct gcggcgctcg
      301 acttgcgtcg gcgtttgccg ctgctctttt tcttggcgcg tgcctgtttg cggtggggtc
      361 ttgcggccgc gggggcgagg tactcgccgt acagcgattc gatctcggcg agcgtcgctt
      421 ctcgaacgga gtagccgtcg tcgcgttcct ctttgcgcaa gctgcgccgc tcgccgtgca
      481 cgaagctcgg cgatggagag cgccgcggcg gaatcctgac ggcatcgtcg agaacgggta
      541 tctcgtcgaa gccgcggacg tattcctcgt aaagagcgtg attggcgggc tcgccgtcga
      601 cgacgagact accgatcggg cgttcggctg ctttcatggc ttcgtgccct ccatgcgact
      661 cgatggaggt caacctacgc gggcggcacg gtacgcgccc ggaaaccggt cacggttgcg
      721 cggtttgacg cgtcacggca ctgcggtttg aatgccgagc accccaagac tgccgagcgc
      781 cgccgtcatc gcgcgttcgc cttcgcggat ggcttcgtcg gcgcgatgaa aatccagcaa
      841 cccgatgtcg gcgagacgcg gcgcaaccat gaggtccggc ggctcgccgg ccatgcggct
      901 gcgcgtgatg cgaacctgca tgacgtcgag gctcgtggcg acgacgttca agacggacgg
      961 tagacgtggg gaattctcgc cccacaagcg gccgaagccg cggcgcatcc actcgccgac
     1021 aaaggtgttg cggctcacgt gagcctcctc gacgacgagg ctgcggccga cgataccggc
     1081 gctcaagtcg actgcgatca cgatgtcggc acccatggcg cgcgccagcg agacgggcac
     1141 gggattcacg agcgcgccgt cgacgagaat tccgtcgctg ctcatgacgg gcgtaaagag
     1201 gcctggcatc gcgagcgagg cccgcaccgc gttcttcgtc gagccggttc gcagccacac
     1261 ttcggcgccg ctctcgagcg acgtcgctac ggcggcgaac ggtatcgcga gctcttcgat
     1321 tggacggtcc tgccaggctc ggctcaagta cgccatgaag cgttcgccct tgagcaaacc
     1381 gccgctcatt gcgacgtcca tgaaggacac gacgtcgcgg cggcgcatgc cggcagtgcc
     1441 ggcgcagaag cggtcgaaat cgccggatgc gtaggcggcc ccgacgaggg cgccgacgga
     1501 tgtgccgcag acgagatggg gacgtacacc cgccttctcg agcgcgcgaa tgacgccgat
     1561 atgggcccag ccgcgcgctg cgccgctgcc gaggacgagg ccgactcgcg gtgcccggcg
     1621 tttcgccgtc gacagccgaa cgtcggctag ctcagcaacc aagacgcaac ctcctacgaa
     1681 caccccgtcg ctcgcgggcg atcccggcga gctaatggag taactgcaag ttgcgtgccg
     1741 gcgggccgct gagcggagct tcagcgccgc atcttgagca tcgcgccgcc gaagaagccc
     1801 tcgacgctgt cgaggtcgat gattagcgca atgcctttgc ggtccttgtc cttgtcgcgc
     1861 tcgaatgcgt gggcgcgctc gtagatgtgg cggcggccgc tttcgctcga gtcgatgcgc
     1921 gcgcggccgc gaaaaatcat gaaggctcgg cccgtcggct cgaagttgcc gtacaggagc
     1981 gccatggctg ggttcttcgt gatcgattcg agtattcggc cctcgggatt gcggacccag
     2041 atcgcgagct gcgtgtcgct gtaagcctga gtgctgccac ggaaggacag ctgcggcgcg
     2101 ccgctctcgt cgacgtacga cacgacgatc ggcttcagcg actcgaacgc cgtgtcgacg
     2161 gctttcttca tgtcggcgga caacttgagc tcggtcagaa ttggcggtgc gctcatcgaa
     2221 cgactccttg gctgcggcaa gcgcgctagg gtagcgcgcg cggcgccgcc agcataccgg
     2281 cgaggtgagc tgcaatgtgc gcgttcgggc aacgcgaggc gccaattctc gcgcaaggct
     2341 tcgcgacgtt gccgcgcagc cacggcgtcg tcgatcgcag acggctcggc tggcacgtga
     2401 cttgcctctt tcagcgacgc aaatcttctt ctcgcgagtc gcgagcgact tcagttcaag
     2461 gaggaacgat gatcgcgcac acgggtctgg ccgtccgaga gttcgcggcc gccaaaaaat
     2521 tttacgagcg agcgctggcg cctctcggct actcggcgaa gatggaagtc ggcgagtccg
     2581 ccggcttcaa cgacggtaag aacaccgatt tctggatcgg ccacagcaac gccgtcgagc
     2641 ccggccacgt cgcgttcgag gcgaaaagca aacgcgaagt cgacgccttc tatcgcgcgg
     2701 ccctcgcggc cggcggacgg gataacggcg agcccggtta ccgtccgcag tactggcccg
     2761 gttattacgc cgcgttcgtc tacgacctcg acggccacaa cgtcgaagcc gtctggtacg
     2821 actacgctgc ctcgaacgag tgagttgagc cacgccggag gactgcatca tggaagagag
     2881 cgagcacctt tgcgatctcc tgaagggctt cgacacggcg atgttggtga cgcgcgccag
     2941 ggacggctat ctgcacgcgc ggccgatggc cgtcgccgag ctgcgcgccg atgccgacgc
     3001 ctatttcgtc acgagcatcg actcgccgaa gattgccgag atcgacgccg acgcggacgt
     3061 cttgctgacg tttcagagcg agcgccaata cgccgctgtg tacggccgcg tgaacgtcgt
     3121 ccaagaccga cagctcatcg agcggctatg gaaagaagaa tggaaggttt ggtttccgca
     3181 gggtaagtcg gacccttcga tcgcgctgtt gcgcttcgac gcggagcgcg gcgagttctg
     3241 ggacaacgcc ggcacgcgcg gcgtcgagta tgcgttcgag gcggcgaagg cttacgtgcg
     3301 cggcgagaag ccgcacgtcg atagcgatca acacgggcgc gtgcgcctca gctgagctag
     3361 accagcgcga cccacgtata agcgggcgac caatcgagtg cgagcgcggt tagggacgct
     3421 tgggccaggg cgagcgccga acgcaacggc gtcagcaagc cgcgcgactc gtaccaggcg
     3481 gcagtccggc gcacgccctc ggccaccttt acgcgtggct cgtagccgat gagctgtcgc
     3541 gctttcgtga tgtcgaactc gcgatggtgc gtaaagaaac cgacgcggcg ccggtgcagc
     3601 ggcggctcga cgcgcagccg cacgcacacc atctcgcaga gcgacgcagc ggcgtagacc
     3661 ggccaaacgg gaacgcgcaa ccatggacgc gccgcgccaa cggcatccgc gactgttgct
     3721 gcgatctcgt tcagggtagg cgcctcgggt cccgcgatga ggaacgtctc gccgcgcgcc
     3781 tcggggcgcg agcccgcgag cacgattccg tccacgaggt cgtcgacgtg caccatgtgc
     3841 aaccgtgttt cgccggagcc gaccatcgcg aagcgctgcc gctggatcgc gcggaacagc
     3901 cgcagaaatc gcatgtcgcc ttcgccgtag atggcgccgg gccgaacgat cgagatcggt
     3961 agtccgttgt tcgatgcttc ggccacggca agctcggctt cgagcttcgt gcgctgatag
     4021 acgtcgccgg ggcggaatcg atacgtctcg gcagccggtg gttgctcgat gtgcccgtgc
     4081 acgccgcccg tagagcaatg aacgacgcgc tcgcagcctt gtgcgcgcgc tgccgcgagc
     4141 acgttcatcg tcccgccgac gttgacgtcc cagtaatgct ggtccgagtg cttgacgttg
     4201 cgaaatgctg cggccaagtg atagatgcgc tcgcagccat tagccgcctc acgcacgtct
     4261 gcagcgttcg taagttggcc ctcgaagatt tcgattccgg attccgcgag gcgccggccg
     4321 cgccgtccgg ggcgcacgag tgcgcgcacg gcgtgacccg atgctgagag cgcacgggcc
     4381 aggtgtttgc cggcgaagcc tgtggcgccg gtcacgagca ctttcactgc agctctctcc
     4441 tatgtctcgg ccggcacgcc ggccggcacg cgtaagtggc gcacgacgag cgcgaacgac
     4501 aggccggccc acgcgagcca gccgacgcct acgccgtaga tcacgcccgt gaggccccag
     4561 cgcgccgcaa cgacggcgga ccccacggcc agcgccacgg aaaaccagcc ggccgcattg
     4621 acgagcgcca gctcgcgctg cgtcgccaga gccgtggccg cggctttggc gaatgcatgt
     4681 gcgatctttg cgatgccgct gaacagcacg gcgaccacga ggccaagcgg caagtggtac
     4741 ttgcccgcca gcagccatcg ctcgatcagc ggcgtcagca ccaagatggc gatcgcgcct
     4801 gcggccgaga tcacgacggc cagccgcaat tcgcgcgcga tgagctgccg gcgctcgagc
     4861 accgtcggtg cgctacgcaa gcgcggcaac agcgtgaagc cgacgcccat ctgcagtaga
     4921 cgaaacagcg accccgcgat cgctccgagc acgccgaaga gtgccaagtc ggcgagcggc
     4981 agcacgtacg gaatcagcag ccgctcgagt tgaatcagca gcattccggc ggcggcaacg
     5041 cctgctaacg cgaacgcctc gccccacgga atcgcggtcg ccagcgaagt cttccctcgt
     5101 cgctcgcgca gcacgagcgc ccagctcagg cccgccatca gtgccagccc gaccgcgaga
     5161 atcgcaaacg gcagcgtcgc ggtttttgct tcgaccgtca gcgtcgcgag cgcccccagc
     5221 atcagcagca ggttgggtga attcagcagc gccagcgaga cggtgaagcg ctgctcgctt
     5281 tgaaatttcg ccgcggcggc cagcatcaag ccgccggcga gtgtcgcccc gagcagcaac
     5341 gcggcggttg ccggcgatag gccgtaggct gcgataccca cagcgcagca ggcgctggcg
     5401 acggaggtgg ccgtcacggc gacccgcttc aagagcgcgg gccccgcgtc gactcggccg
     5461 cgaatcacga ccgcgtcgag gccggcgggc gcgaggtggt agccgagggt gctcagcgca
     5521 acgagcagcg tgaccaacgc gtactggtcc gtcgacagca cccgcgcgag cagcagattg
     5581 gcgcccgcga acgctacccc cgatgccccg tacacggcca tgctgcgcaa ggtcggcgat
     5641 ggcgcgtagc gcattctttc cgtttctccc gatgactgca ggtacgcctc cgccgtccgg
     5701 cggagggcgc taacgcgtcg caaatagcaa gcaatcatcg tgccagccgg cgcggatgcg
     5761 ccgtccgagc ttaagccggc cgtgcgccga acgtgccggc aacgtggggt aaaatgcggc
     5821 taaaagcggc tatcgcgtcg ccttcagcca tttcggcgca aaagcaaagg ccgccggcct
     5881 gccggcggcg ttgttcagtc gagctactgc gccttttcgg cgtcagcttt agacgtgaag
     5941 cccttggcca gccagtcctt ccagcggttg acgttctgct cgttacccgc gcacccgccg
     6001 tcggccggat tgatgtactg cccgtcctcg agtttcgccc agccgatcgc gccgccgggc
     6061 cggccgtcgc gcagcgggca atacatgaac tgcaccgtgt cgccaggctc gaacgtcgcg
     6121 cccgtgatgc cgcgctggat cagcgccgga ggtgccgagc cttcgaagct ccacagcgtg
     6181 tcggtgccat cggcgttctt gacgttcaag tacagccacg agtgcggatt gttgaatgcc
     6241 caccgaacga cggtgcccgt ttttacgatc tcttgctcgc ggtcgaacat cgcgaaggag
     6301 tggtgcgcct ccgcggccgc catcgtcagc agcgcggcgg ccgagacggc cggcaacagt
     6361 ttcacaatcg atttcattga gagctccttc actagttgcc taacgtcgtg ttgtagatcg
     6421 gatcgcgggt ctggccgggc aagtcgggga acaacgtcgt gccgcggccg tcgttgaatc
     6481 ctgcttcgcc gggcaggtgg aaattggtta cgcctt
//
LOCUS       DATLEE010000386         7344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8413565, whole genome shotgun sequence.
ACCESSION   DATLEE010000386 DATLEE010000000
VERSION     DATLEE010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8413565"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3845)
                     /locus_tag="VM692_08285"
     CDS             complement(<1..3845)
                     /locus_tag="VM692_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HUQ52207.1"
                     /translation="MADAPPLVLEHLTTEDGLPQATVMTTLQDSQGFVWLGTEDGLVR
                     FDGRELYRYARSRTESGSLPGNYVWQIAEDAATNLWIALNDSGLAKWDRRTDRFTSYR
                     HDDSDPRSLASDRVRTVLLDGKGSVWIGTFDAGVDVLDPSSGSIEHLRHDANNAGSLA
                     SDRVVTLMLDRAGDVWIGTDHGLNRWHSATRTMTRVGPPGEPRSLPDEQISRLLEDQD
                     GTVWVGSFESGLVRMDRDGNVLEMFRHDASDPTSLGSDDVRALLEDRSGRFWVGTADG
                     LALLDRFTGTFSHYRHEANDNASLRDSFVMSLYQDPAGLVWVGTRTGGVSRWNPRSWE
                     LGGNRPAWLENQPITAFADAPDNQLWIASLAGLVRFDVATGRGTSIDTLVGRTNAVGD
                     APVTSLRQDRRGGLWIGTMGGGLKLLTPDARLESIPVEAGDSRSLSTDGMLAILESRS
                     GAIWIGTYGGGVNVLDPATRTVRQLPYGSGSPGALSAPIVTAIAEDARGYLWLGTEGG
                     GLNLARADGTVLGVYRNDPADASSLPSNTVYGLAVDQDGGVWVATDAGGLARVVDPTA
                     APDKIKFRILSRADGLSSDTLYGIVPDARGRLWLSGNAGLMRLDPATGSVKTYHREHG
                     LQGEEFSFGAYFRLRDGRVAFGGSGGFNIFDPAALSENAEPPRIALTNVDVLGVRAAG
                     ATPFWLRDRIALDYRGTIVSLDFGVLDFNSPTHNRLAYRMTGLTNDWIDLGAQRRVTL
                     TNLDPGDHVLEVRAANSDSVWSEAPLLLTIHRDPAPWASPLAYALYTLALLAFAARRW
                     QLHRRKLREMVQARERLEAEVQLRTRELTESNRQLAEAARAKSDFLDRMSHELRTPMN
                     GVVGMTELLSRTALSATQSHLTKTIRSSAQILLQIVNDLLDLSKNRAGKVALERLPIE
                     LGQVLEECTSIFAGTADNKGIELIVCPPPPAQRVLLGDPLRLRQVLMNLVGNAVKFTE
                     RGEVVVRADVEHVDGDPPFGGFQGSRATVRLAVTDTGIGMDAATTAKIFEPFAQADET
                     TTRRFGGTGLGLAICRELADLMSGSITVESQPGVGSTFRLSLPMVLGDAIPHAPLLPP
                     PLGVPEGRRRTVRIVTRRPSLADSIARHATALGLDVLPTEPETAEADVVVVDATTRPG
                     TLSSLLTSDGVTHPALVVIATSADIESRALRLLLPEQQIVLKPVHGTALREAFASALG
                     APRAAERAEPALSSGDFQGSRSASGPLKAHVLLVEDEPVNAAVAEGYLEALGCTATWV
                     TSGAEAVACAAAEHFDVIF"
     gene            complement(4027..5883)
                     /locus_tag="VM692_08290"
     CDS             complement(4027..5883)
                     /locus_tag="VM692_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUQ52208.1"
                     /translation="MKRVLPISPVLAALCAAMAWTGIALAQAAPSALYIVQSATLDDA
                     RVQVQRVGGAPERDFEIIHAVAVRLTADQADRLRKSSGVRVFDDREVKTRGTLLGGLQ
                     DVVNNVNSTLASTPLVQTVQSVAAPVVSAVACNAALNPVTSPLVSTLTSVQNSTQLAS
                     LPLAYETNYPAMIGADKLHCSGITGRGVTIAVLDTGLWRDTFQFYGTRVLATVDVTNG
                     GRGPVTGDPYGHGTHITSVAASGAQALNGSYQGIAPQANLVVVRAFDGTGAGRYSDVI
                     AGLDWIVANRTKHKIRVLNLSFGAAPQSFYWDDPLNQAVMAAWRAGIVVVVSAGNDGP
                     DPMTINVPGNVPYVITVGALTDSNTPYNYADDKLASFSSTGPTYEGFVKPELVSPGGH
                     IKASMSSSSFLAYLDPKSVQATNQLFTMSGTSQAAAVTSGVVALMLQDDPWLTPDDVK
                     CRLIAAARPAVKTNGKLAYSVFQQGAGLINARDAVTSTATGCANRGLDIGDDLAGTKH
                     FGGPANEENGQYYIMDMSGSTWGDSLDADGYTWSRGYPWGQGYAWSGGYTWSRGYTWS
                     RGYTWSRGYTWSRGYTWSRGYTWSRSLNWWSAPATFVPMTKPASTVTFVYNE"
     gene            complement(6018..>7344)
                     /locus_tag="VM692_08295"
     CDS             complement(6018..>7344)
                     /locus_tag="VM692_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ52209.1"
                     /translation="CVVRPVLAAGSNAGLESTGEHRQLQLDRRGFLRRFKESISVAAL
                     RERPVAVAVLYIDEIADIAEIIATRISEQIMSTALARLPTPSGGSDAPDWYLGQLGEN
                     LLALVVESADREIVEGCVATVCASLREPVSVGDAEFRLTPYAGVSILGVDASSPRVLL
                     DHARGAAAEARRAASRDVFFFSDTLQLKSLARIDIARELREAIASRAIRLRYTGRHDL
                     RTGRLVAWVGYVRWLHPLRGEIRPAEFLRVAQTTGLATSLSRSVLEHLPQDFAELGAK
                     AESDVRISLGALRDHVLHEDFTTDVDRFLAASGVPPERLELRIAEKVFVARDSADLKS
                     LQKRGVQFVVDEVGRSISSLTSLARAPVWGLQLDRAWVQALRTDAVARNVCHAGIAMA
                     SALGLTAIATGVDDAAQRSALLELGCRLGIGDLYGGLGTRAVAPAVERA"
BASE COUNT         1134 a         2498 c         2574 g         1138 t
ORIGIN      
        1 atgaagatga cgtcgaagtg ctcggccgcg gcgcacgcca cggcttcggc gccgctcgtg
       61 acccacgtcg ccgtgcagcc cagcgcctcg aggtaaccct cggcgacggc cgcgttgacc
      121 ggctcgtcct cgacgagcag cacgtgcgct ttcaaaggac cgctggccga gcgcgaccct
      181 tggaaatccc cggaagagag tgccggctcc gcacgctcgg ccgctcgcgg agcaccgagc
      241 gccgacgcga atgcctcgcg cagcgcggtg ccgtgcacgg gcttcaacac gatctgctgc
      301 tccggcagca gcagccgcag cgcgcgcgac tcgatgtcgg ccgaggtcgc gatgacgacg
      361 agcgccggat gtgtcacgcc gtcggacgtc agcagtgagc tcaacgtgcc cggccgagtc
      421 gtcgcgtcga cgacgacgac gtcggcttcg gccgtctccg gctcggtcgg caaaacgtcg
      481 agcccgagcg ccgtcgcgtg gcgcgcgatc gaatcggcca gcgacggccg ccgcgtgacg
      541 atgcgcaccg tgcgacgcct tccttcgggg actcccaagg gcggcggcag cagaggtgcg
      601 tgcggaattg cgtcgccgag caccatcggc agcgacagcc ggaacgtcga cccgacgccg
      661 ggttggctct cgacggtgat gctgccgctc atgaggtcgg cgagctcgcg gcagatcgcg
      721 aggcccaatc ccgtgccgcc gaagcgccgc gtcgtggtct cgtcggcttg cgcgaacggc
      781 tcgaagatct tggccgtcgt ggctgcgtcc atgccgatgc ccgtgtcggt cacggcgagg
      841 cgcacggtcg cgcgcgaccc ttggaagccc ccgaaggggg gatcgccgtc gacgtgctcg
      901 acgtcggcgc gcacgacgac ctcgcctcgc tcggtgaact tcacggcgtt gccgacgagg
      961 ttcatgagca cctggcgcag ccgcagggga tcgccgagca gcacgcgctg cgcgggcggc
     1021 ggcggacaga cgatcagctc gatgcctttg ttgtcggccg tgccggcaaa gatgctcgtg
     1081 cattcctcga gcacctggcc gagctcgatc ggcagcctct cgagcgcgac cttgccggcg
     1141 cggttcttcg acagatcgag caagtcgttg acgatctgca gcaggatttg cgccgaggag
     1201 cggatcgtct tcgtcaggtg cgactgcgtc gcggacagcg ccgtgcgaga caggagctcc
     1261 gtcatgccga cgacgccgtt catcggcgta cgtagctcgt ggctcatgcg gtcgaggaag
     1321 tcgctcttcg cgcgcgcggc ttcggcgagc tgccggttgc tctcggtcag ctcacgtgtg
     1381 cgcagctgca cctcggcttc gagacgctcg cgcgcctgca ccatctcgcg cagcttccgg
     1441 cggtgcagct gccagcgccg agccgcgaac gccagcaacg cgagcgtgta gagcgcatac
     1501 gccagcggcg aagcccacgg ggccggatcg cggtggatcg tgaggaggag cggcgcctcg
     1561 ctccacacgg aatccgagtt cgccgcgcgc acctcgagca cgtgatcgcc agggtccagg
     1621 ttcgtcagtg tcacgcgccg ctgggcgccg agatcgatcc agtcgttcgt gaggcccgtc
     1681 attcggtagg cgagccggtt gtgcgtaggc gagttgaaat ccaagacgcc gaagtcgagc
     1741 gagacgatcg tgccgcggta gtcgagcgca atgcgatcgc gcaaccagaa cggtgtcgcg
     1801 ccggcggccc ggacgccgag tacgtcgacg ttggtcagtg cgatgcgcgg cggctcggcg
     1861 ttctccgaca acgccgccgg atcgaagatg ttgaacccgc ccgacccgcc gaatgcgacg
     1921 cgcccgtcgc gcaggcgaaa gtacgcgccg aacgagaact cttcgccctg cagcccatgc
     1981 tcgcggtggt aggtcttgac gctgccggtc gcggggtcga ggcgcatcag tccggcgttg
     2041 ccgctgagcc atagccggcc tcgcgcgtcg ggcacgatgc cgtacaacgt atcgctcgac
     2101 agaccatcgg cgcgcgacag gatccggaac ttgatcttgt cgggcgcggc ggtcgggtcc
     2161 acgacgcgcg cgagcccacc ggcgtcggtg gcgacccaga ctccgccgtc ctggtcgacg
     2221 gcaagcccgt acacggtgtt cgacggcagc gacgacgcgt cggccgggtc gttgcggtac
     2281 acgccgagca cggtgccgtc ggcgcgcgcg agattcagac cgccgccttc cgtgccgagc
     2341 cacaagtagc cgcgcgcgtc ctcggcgatc gccgtgacga tcggcgcgct gagggcgccg
     2401 gggcttcccg agccgtacgg cagctgccgt acggtccgcg tcgcggggtc gagcacgttg
     2461 acaccgccgc cgtaggtgcc gatccagatc gctccgctgc gcgattcgag gatcgccaac
     2521 atgccgtccg tgctcagcga gcgcgaatcg ccggcctcga ccgggatcga ctcgaggcgt
     2581 gcgtctggtg tcagcagctt gaggccgccg cccatcgttc cgatccagag tccgccgcgt
     2641 cggtcttgcc gtagcgaagt gacgggcgcg tcgccgacgg cgttggtgcg gccgacgagc
     2701 gtatcgatcg aggtgccgcg gcccgtcgcg acgtcgaagc gcacgaggcc cgcgagcgac
     2761 gctatccaga gctggttgtc cggcgcatcc gcgaacgccg tgatcggttg attctcgagc
     2821 cacgccggcc gattgccgcc gagctcccag ctgcgcgggt tccaccggct cacgccgccc
     2881 gtgcgcgtgc cgacccaaac gagccccgca gggtcttggt acagcgacat cacgaaagag
     2941 tcgcgcagcg acgcgttgtc gttggcctcg tgccggtaat ggctgaaggt gcccgtgaaa
     3001 cggtcgagca acgcgaggcc gtcggcagtg cctacccaga accggcccga ccggtcctcg
     3061 agcaacgcgc ggacgtcgtc gctgccgagc gaggttggat cgcttgcgtc atgccggaac
     3121 atctcgagaa cgttgccgtc gcggtccatg cggacgagcc cactctcgaa gctcccgacc
     3181 cataccgtgc cgtcctggtc ctcgagcagt cgcgagatct gctcgtcggg cagcgagcgc
     3241 ggctcgccgg gcgggccgac gcgcgtcatg gtgcgcgtcg ccgagtgcca gcgattgagg
     3301 ccgtggtccg tgccgatcca gacgtcgccg gcgcggtcga gcatcaacgt gacgacgcga
     3361 tcgcttgcga gcgagcccgc gttgttggcg tcgtgacgca agtgctcgat gcttcccgag
     3421 gacgggtcga gcacgtcgac gccggcgtcg aacgtgccga tccagaccga cccctttccg
     3481 tcgaggagca cggtccgaac gcggtcgctc gcgagcgagc gcgggtcgct gtcgtcgtgg
     3541 cggtagctcg tgaagcggtc ggtgcggcga tcccatttgg cgaggcccga gtcgttaagc
     3601 gcgatccaga ggttggtcgc ggcgtcctcg gcgatctgcc agacgtagtt gccgggcaaa
     3661 gagccgctct ccgtgcgaga gcgcgcgtag cggtagagct cgcggccgtc gaagcgcacg
     3721 aggccgtctt cggtgccgag ccagacgaaa ccctgcgagt cctgcaaggt cgtcatgact
     3781 gtcgcttgcg gcagcccgtc ctcggtcgtg aggtgctcca aaacgagcgg aggggcgtcg
     3841 gccattgcgg cggacgcccc taccactgcg agcagagctt gggccaagcg cgagcaccat
     3901 gcggcgcccg ctcgttcgat tgtcacggcg tcgagtctct ccggcggcag ttcgcggcgc
     3961 gttgatccgc gtcgggaatc ctgcgtcagg ccggaattcc gcagcggaac tgcgtcacat
     4021 tcgaagctat tcgttgtaga cgaacgtgac ggtcgacgcg ggcttggtca tcgggacgaa
     4081 cgtcgccggc gcgctccacc agttgaggct gcggctccag gtatagccgc ggctccaggt
     4141 gtaaccgcgc gaccaggtgt aaccgcggct ccaggtgtaa ccgcgcgacc acgtgtagcc
     4201 gcggctccac gtatagccgc cgctccacgc gtagccctga ccccacggat agccgcgcga
     4261 ccacgtgtag ccgtcggcgt cgagcgagtc gccccaggtc gagccgctca tgtccatgat
     4321 gtagtactgg ccgttctcct cgttggctgg gccgccgaag tgcttcgtgc cggccaggtc
     4381 gtcgccgatg tcgagaccgc gattggcgca gcccgtcgcc gtgctcgtaa cggcgtcgcg
     4441 cgcgttgatg aggccggcgc cttgctggaa cacgctgtac gcgagcttgc cgttcgtctt
     4501 gacggcgggg cgcgcggctg caatgagccg gcacttgacg tcgtcgggcg tcaaccacgg
     4561 gtcgtcctgc agcatcagcg ccacgacgcc cgaggtaacg gccgcggcct gcgacgtgcc
     4621 cgacatcgtg aacagctggt tcgtggcctg caccgacttc gggtcgaggt acgcgaggaa
     4681 gctgctgctc gacatcgagg ccttgatgtg gccgcccggc gagacgagct ccggcttgac
     4741 gaagccctcg tacgtcgggc ccgtcgacga gaacgacgcc agcttgtcgt cggcgtagtt
     4801 gtacggcgtg ttgctgtccg tcagcgcgcc gaccgtgatg acgtacggca cgttgcccgg
     4861 cacgttgatc gtcatcggat ccgggccgtc gttgcccgcg gacacgacga cgacgatgcc
     4921 ggcgcgccaa gcggccatga cggcctggtt cagcggatcg tcccagtaga acgactgcgg
     4981 cgccgcgccg aacgacaggt taagcacgcg gatcttgtgc ttcgtgcggt tcgcgacgat
     5041 ccagtcgagg cccgcgatga cgtccgagta gcggccggcg ccggtgccgt cgaaggcgcg
     5101 cacgacgacg agattcgctt gcggcgcgat gccttgataa gagccgttca acgcctgcgc
     5161 gccgctggcc gcgaccgacg tgatgtgcgt gccgtgaccg tacgggtcgc ccgtcacggg
     5221 accgcgcccg ccgttcgtca cgtcgaccgt cgcgaggacg cgcgtgccgt agaactgaaa
     5281 cgtgtcgcgc cagagacccg tgtctagcac ggcgatcgtg acgccgcggc ccgtgatgcc
     5341 tgagcaatgc agcttatcgg cgccgatcat cgccgggtaa ttggtctcgt aagccagcgg
     5401 caacgaggcg agctgcgtcg agttctgcac gctcgtcagc gtgctgacga gcggcgacgt
     5461 gacggggttc agcgcggcat tgcaggcgac ggccgagacg accggcgccg ctaccgactg
     5521 caccgtctgc accaagggcg tcgaggcgag cgtcgagttg acgttgttga cgacgtcctg
     5581 cagcccgccg agcagcgtgc cgcgcgtctt gacctcgcgg tcgtcgaaga cgcgcacgcc
     5641 ggagctctta cgcaagcggt cggcttggtc ggccgtcagc cgcacggcga cggcatgaat
     5701 gatctcgaag tcgcgctcgg gcgcgccgcc gacgcgctgc acctgtacgc gcgcatcgtc
     5761 gagcgtcgcg ctctgaacga tgtacagggc ggacggtgcg gcttgcgcca gcgcgatgcc
     5821 ggtccaagcc atcgcagcgc aaagcgccgc cagcacgggg gagatcggta gcacgcgttt
     5881 catagttctt ctcacggcga ttcgatgcgt gcatttcagt ctttttgggt ttcgcgcgcg
     5941 ccgccgtggc aaacgcgcgc ccggggagtt tttgtgacgt gcgtcacata acggcgcgtg
     6001 tcaaacgccg gcgactctta ggcgcgttcg acggccggcg cgacggcgcg cgtgccgagc
     6061 ccgccgtaga gatcgccgat gccgagccgg cagccgagct cgagcagcgc gctacgctgc
     6121 gcggcgtcgt cgacgcccgt cgcgatcgcc gtaagaccaa gcgcgctcgc catggcgata
     6181 cctgcgtggc agacgttgcg cgcgacggcg tccgtgcgca gtgcttgcac ccaggcgcga
     6241 tcgagctgca gaccccagac gggagcgcgg gccaacgagg tcagcgagct gatgctgcgg
     6301 cccacctcgt ccactacgaa ctgcacgccg cgcttctgca acgacttgag gtccgccgag
     6361 tcgcgcgcca cgaacacctt ctcggcgatg cgcagctcga gccgctcggg cggtacgccg
     6421 ctcgccgcga ggaaccggtc gacatcagtc gtgaagtcct cgtgcagcac gtggtcgcgt
     6481 agggcgccga gtgagatgcg cacgtcggac tccgcctttg cgccgagctc ggcgaagtct
     6541 tgcggcaaat gctcgaggac gctgcgcgac aagctcgtcg cgagccccgt cgtctgcgca
     6601 acgcgcagga actcggccgg ccgaatctcg ccgcgcaacg ggtgcaacca gcgaacgtag
     6661 ccgacccacg cgacgagacg tcccgtgcgc aggtcgtgcc ggcccgtgta acgcaggcga
     6721 atcgctcggc tcgcgatggc ttcgcgcagc tcgcgcgcga tgtcgatgcg tgccagcgac
     6781 ttcagctgca gcgtgtcgct gaagaaaaag acgtcacgcg aggcggcgcg gcgcgcctcg
     6841 gccgccgctc cccgcgcgtg atcgagcagg acgcgcgggc tcgacgcatc cacgccgagg
     6901 atgctgacgc cggcgtacgg cgtcagccga aactccgcgt cgccgacgct gaccggctcg
     6961 cgcaggctcg cgcagacggt cgcgacgcag ccctcgacga tctcgcgatc cgcgctctcg
     7021 acgacgagcg ccaacaaatt ctcgccgagc tggccgagat accagtccgg tgcatcgctg
     7081 ccgcccgacg gcgtcggcaa ccgcgcgagc gccgtgctca tgatttgctc ggagatacgt
     7141 gtcgcgatga tctccgcgat gtcggcgatc tcgtcgatgt agagcacggc gacggcgacg
     7201 gggcgctcgc gcagcgccgc gacggagatc gattccttga agcggcgcag gaaaccgcgc
     7261 cgatcgagct gcaattgccg gtgctcgccc gtcgactcga gcccggcgtt gctgccggcc
     7321 gccagcacgg ggcgcacgac gcag
//
LOCUS       DATLEE010000387         2951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10653704, whole genome shotgun sequence.
ACCESSION   DATLEE010000387 DATLEE010000000
VERSION     DATLEE010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10653704"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VM692_08300"
     CDS             complement(<1..1050)
                     /locus_tag="VM692_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HUQ52210.1"
                     /translation="MKMTSLAIVALLAVPALAQQRVPELRFTSVPDYPTLPDGMNFGE
                     VPGVAVNSRGHVFVFSRSNSATGPAFGAAAAQLFEFDDNGTFVREIGKGLYAWSEAHS
                     VRIAPNDDIWAIDKGSNMVVRFNPQGRVVWVFGRKEEAADGAEPLEHPDPPLPPVDGM
                     FRQPTDVAWDSQGNTYITDGYVNSRVAKYDKNGDWVKSWGTKGSGPGQFNIPHAIAID
                     RQDNVYVGDRTNHRVQVFDTDGNFKRMFSIDMPPDPASRPVNGATPTGEALSRAIGAP
                     NSICITPGSPQVLFVGESTYPGRIFKVALDGTVLGVLGRRDDVTRWATSNDLRVLARL
                     RNFGEMFNPSRAGIAE"
     gene            complement(1066..1818)
                     /locus_tag="VM692_08305"
     CDS             complement(1066..1818)
                     /locus_tag="VM692_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52211.1"
                     /translation="MSASAASAADVTDRAFKPSFFFWMTVVMAFFVFTGFGMTYLFPL
                     TRGTFPPAPPIVHLHGFVFFTWMILLIVQAALINSGNVALHRSLGTWGIAHATVILYT
                     GALIQLLGIARSIRNGGTGPFDAGFLGLMAVVGFALLFTLAIRNVRRPEVHKRLILFA
                     MLPILPPGVNRFWQVPFGLDYIPVLPLYVTLDSLAAAILVHEWRSTGKIGKYSMIGAG
                     WIFGQSLIHVPVVHSDWFLEAVRFVAGLIRYR"
     gene            complement(1844..>2951)
                     /locus_tag="VM692_08310"
     CDS             complement(1844..>2951)
                     /locus_tag="VM692_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013051597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HUQ52212.1"
                     /translation="YDQLHCYVRAELNEEYGDDVVPLDQPIRADLLGNMWAQTWAPLY
                     DLAAPPNASTGIDLDKLLVEKDYDPIKMTKTAEAFFSSLGFEPLPDTFWERSQITRPE
                     GREVVCHASAWDLDSTDDLRVKLCMEVNAEDFRTIHHELGHNYYQRAYKDQPVLFRGG
                     ANDGFHEAIGDTVMLSITPEYLREIGLIESVPDASQDLGLLMQQALDGVAFLPFGLLI
                     DKWRWQVFSGELTPDTYNTGWWALREQYQGVRPPVDRPADAFDPGAKYHIPANVPYMR
                     YFLARVLQYQFYKAACDTAGWTGPLHRCSFFGSKEVGARLNAMLELGLSKPWPEALEA
                     FTGTRTIDGSAILAYYAPLQAWLEQQNAGRTCGW"
BASE COUNT          524 a          952 c          980 g          495 t
ORIGIN      
        1 ctcggcgatg cccgcgcggc tcggattgaa catctcgccg aagttgcgca gccgcgcgag
       61 gacgcgcagg tcgttcgacg tcgcccagcg cgtcacgtcg tcgcggcggc cgagcacgcc
      121 gagcacggtg ccgtcgagcg ctaccttgaa gatgcgtccc ggatacgtcg actcgccaac
      181 gaacagcact tgcggtgagc ccggcgtgat gcagatcgaa ttcggcgcgc cgatcgcgcg
      241 gctcagcgct tcgccggtcg gcgtcgcgcc gttgacgggg cgcgaagcag ggtctggcgg
      301 catgtcgatc gagaacatgc gcttgaagtt gccgtcggtg tcgaacacct gcacgcgatg
      361 gttcgtgcgg tcgccgacgt agacgttgtc ctgacggtcg atcgcgatcg cgtgcggaat
      421 gttgaactgg ccgggcccgc tgcctttcgt gccccacgac ttcacccagt cgccgttctt
      481 gtcgtacttg gcgacgcgtg aattgacgta gccgtcggtg atgtaggtgt tgccctgcga
      541 gtcccacgcc acgtcggtcg gttgccggaa catgccgtcg accggcggca gcggcgggtc
      601 gggatgctcg agcggctcgg cgccgtcggc cgcttcttcc ttgcgtccga acacccagac
      661 gacgcgcccc tgcgggttga agcgcacgac catgttcgag cctttgtcga tggcccaaat
      721 gtcgtcgttg ggagcgatgc gcacgctgtg cgcctccgac caggcgtaca gccccttgcc
      781 gatctcgcgc acgaacgtac cgttgtcgtc gaactcgaac agctgcgccg ccgctgcgcc
      841 gaacgcggga cccgtcgcgc tgttcgagcg cgagaatacg aacacgtgcc cacgcgagtt
      901 cacggccacg ccgggcactt cgccgaagtt catgccgtcc ggtaacgtcg gatagtcggg
      961 gaccgacgtg aagcggagct ccggcacgcg ctgctgcgcg agcgccggca ccgcgagcag
     1021 cgccacgatc gcgagcgaag tcatcttcat gcgtccctcg ccaggtcagc ggtaacggat
     1081 caagccggca acgaaacgga ccgcttcgag aaaccagtcc gagtgcacga cgggaacgtg
     1141 gatgaggctc tgcccgaaaa tccagcccgc gccgatcatc gagtacttgc cgatcttgcc
     1201 ggtgctgcgc cactcgtgca cgagtatggc cgcggccagc gagtccagcg tcacgtacaa
     1261 cggcagcacg gggatgtagt cgagaccgaa cggcacctgc caaaaccgat tcacgccggg
     1321 cggtaggatc ggcagcatcg cgaagagaat gagccgcttg tgcacctccg gcctacgcac
     1381 gttgcgaatg gccagcgtga acaacagcgc gaaaccgacc accgccatga gcccgaggaa
     1441 cccggcgtcg aacggccccg tgccgccgtt acggatgctc cgcgcgatgc cgagcagttg
     1501 aatcaacgcg ccggtgtaga ggatcacggt ggcgtgtgcg atgccccacg tgccgaggct
     1561 gcggtgcagc gcgacgttgc cgctgttgat caacgcggct tgcacgatca gcaggatcat
     1621 ccacgtgaag aacacgaagc cgtgcagatg cacgatcggc ggcgccggcg gaaacgttcc
     1681 cctcgtcagc gggaacaaat acgtcatgcc gaagccggtg aagacgaaga acgccatcac
     1741 caccgtcatc caaaagaaga acgacggctt gaaggcgcga tccgtgacgt ctgcggcgga
     1801 agcagccgat gcactcatgc cggatatcct cgatttactg gcgttaccaa ccgcaggtcc
     1861 tgcccgcgtt ctgctgctcg agccacgctt gtagcggcgc gtagtacgcg aggatcgccg
     1921 agccgtcgat cgtgcgcgtg cccgtgaacg cctcgagcgc ctcgggccac ggcttcgata
     1981 acccaagctc gagcatcgcg ttgagccgcg cgccgacctc tttcgagccg aagaacgagc
     2041 agcgatgcag tgggcccgtc cagccggcgg tgtcgcaggc ggccttgtag aactggtact
     2101 ggagcacgcg cgcgaggaag tagcgcatgt acggcacgtt ggcggggatg tggtacttcg
     2161 ctccgggatc gaaggcgtcc gcggggcgat cgacgggcgg ccgcacgccc tgatattgct
     2221 cacgcagcgc ccaccagccg gtgttgtacg tgtcgggtgt cagctcgccc gagaacacct
     2281 gccagcgcca tttgtcgatc agcaacccga acggcaggaa cgccacgccg tcgagcgcct
     2341 gctgcatcaa gagccctaag tcctgcgacg cgtcgggcac ggactcgatg agaccgatct
     2401 cgcgcagata ctcgggcgtg atcgatagca tgacggtgtc gccgatcgcc tcgtgaaacc
     2461 cgtcgttggc gccgccgcgg aacagcacgg gttggtcctt gtatgcgcgc tggtagtagt
     2521 tgtggccgag ctcgtggtgg atggtgcgaa agtcttcggc gttgacctcc atgcacagct
     2581 tgacgcgcag gtcgtccgtc gagtcgaggt cccaagccga ggcgtggcag acgacttcgc
     2641 gtccctcggg ccgcgtgatc tgcgagcgct cccagaacgt gtcgggcagc ggctcgaacc
     2701 cgagcgagga gaagaacgcc tcggccgtct tcgtcatctt gatcgggtcg tagtctttct
     2761 cgacgagcag cttgtcgagg tcgatcccgg tgctcgcgtt cggcggcgct gcgaggtcat
     2821 agagcggtgc ccaagtctgc gcccacatgt tgccgagcag gtccgcacga atcggttgat
     2881 cgagcggcac gacgtcgtcg ccgtactcct cgttcagctc ggcgcgcacg tagcagtgca
     2941 gctgatcgta g
//
LOCUS       DATLEE010000388         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1180497, whole genome shotgun sequence.
ACCESSION   DATLEE010000388 DATLEE010000000
VERSION     DATLEE010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180497"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VM692_08315"
     CDS             <1..885
                     /locus_tag="VM692_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ52213.1"
                     /translation="YIEASWSATDRLRFLTGARADLYDFEATAKIAGSAEGHESDSRA
                     SPKFGVAYTVNDNVELYGNWGKGFHSNDARGVVNPVDPVPGLSPGEGYETGARFEVGA
                     LKLTSAYWWLNLDSELIFVGDSNAVEPKGGSRRKGYELTAFWKPIDWLGIDAVYTGSR
                     ARYIDNPDGRYIEGSVEHAGQLGISAVKDKWEASLRVRYLGEYALVPDNSQRAEAETS
                     VNLRGAYTTGKATWYAELLNIFDADGKDIVYWYEAYVAGLDPPGLTSADIDCDVVNCR
                     ISRAEEPRTLRVGVKFEF"
     gene            complement(882..1682)
                     /locus_tag="VM692_08320"
     CDS             complement(882..1682)
                     /locus_tag="VM692_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ52214.1"
                     /translation="MRVQRSSLSFGWAVLAVASLVGCSRDPPPLRFDAAGNFQPATLA
                     EAVLFRNRTAVENFLALGVDPNEPEPDGTTPLMRAVNGETLDIAQLLIDASADVNKAN
                     YYGVTALYIAARDGDASATRMLLAAGANANVALPAGETVLMTAAKAGNPEVVRALLTG
                     GVEAVSLTEIGEARAAAGVAEAAGYSAQTNPALATNYADVNARERWYGRTALMIAALE
                     GHAAVVELLIEAGADPTLVDEEGSTALTLARSSGNLDVAAKLAALASR"
     gene            complement(1769..>2488)
                     /locus_tag="VM692_08325"
     CDS             complement(1769..>2488)
                     /locus_tag="VM692_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52215.1"
                     /translation="KVDVNAAAPRAADGHPDLSGLWERFGPGGQNPDFDALQSADGGP
                     PAATFWNTGAGLRGGLPYTPLAAMLRDERMADNQKNNPDALCLPMGLMQLHLHPQPRK
                     IVQTPGLIVIMYEGNEGLRQIFTDGRALPKAGPELQPWWYGYSTGHWEGDTLVVETVG
                     FRDDVWLDVQGSPLTGAGKLTERYRRPNYGTLQIDITVEDPAAYTKPFTVRVNQRLFA
                     DAELIEFICNENEKSSRFYDR"
BASE COUNT          409 a          811 c          845 g          423 t
ORIGIN      
        1 tacatcgaag cgagctggtc ggcgaccgat cggctgcggt ttttgacggg cgcgcgcgcc
       61 gacctctacg acttcgaggc gacggcgaaa atcgcgggca gcgccgaagg tcacgagagc
      121 gactcgcgcg cgtcgccgaa gttcggtgtc gcctacacgg tcaacgacaa cgtcgagctc
      181 tacggcaact ggggcaaagg gttccattcg aacgacgcgc gcggcgtcgt gaatcccgtg
      241 gatcccgttc ccggcctctc gccgggcgag ggctacgaga ccggcgcccg gttcgaggtc
      301 ggcgcattga agctcacaag cgcgtattgg tggctaaacc tcgacagcga gctgatcttc
      361 gtcggcgact cgaacgccgt cgagccgaag ggcggctcgc gccgcaaagg ttacgagctg
      421 acggcgttct ggaagccgat cgactggctt ggcatcgacg ccgtctatac gggcagccgc
      481 gcgcgctaca tcgacaaccc ggatgggcgc tacatcgaag gctcggtcga gcacgcgggc
      541 cagctcggca tctcggcggt caaggacaaa tgggaagcga gcttgcgcgt tcgctacctc
      601 ggcgagtacg cgctcgtgcc cgacaactca cagcgcgccg aagcggagac ttcggtgaac
      661 cttcgcggcg cctacacgac gggcaaagcg acttggtacg cggagctgct gaacatcttc
      721 gacgccgacg gcaaggacat cgtttattgg tacgaggctt acgtggccgg tctcgatccg
      781 ccgggtctca cgtcggcgga catcgactgc gacgtcgtca actgccgcat cagtcgcgcc
      841 gaggaaccgc ggacgctgcg cgtcggcgtg aagttcgagt tctagcgcga ggcgagcgcc
      901 gcgagcttcg cggcgacgtc gaggttgccc gagctgcgcg caagcgtcag cgccgtcgag
      961 ccttcctcgt cgacgagcgt tggatcggcg ccggcctcga tgagcagctc gacgacggcg
     1021 gcgtggccct cgagcgcggc gatcatcagc gcggtccggc cgtaccagcg ctcgcgcgcg
     1081 ttgacgtcgg catagttcgt cgcgagcgcg ggattcgtct gcgccgagta accggcggcc
     1141 tcggcgacgc cggccgcggc ccgcgcttcg ccgatctccg tcagcgacac ggcttcgacg
     1201 ccgcccgtca gtagcgcgcg gacgacctcg ggattgccgg ccttggctgc cgtcatcagc
     1261 acggtttcgc cggcaggcag cgcgacgttc gcgttggcgc ccgcggccag cagcatgcga
     1321 gtcgcgctag cgtcgccgtc gcgtgctgcg atatagaggg ccgtgacgcc gtagtagttg
     1381 gctttgttga cgtccgcgct ggcgtcgatc agcagctgcg cgatgtcgag cgtttcgccg
     1441 ttgacggccc gcatcaacgg cgtcgtgccg tcgggctccg gttcgttggg atccacgccg
     1501 agcgcgagga agttctcgac ggccgtccga ttgcggaaca acaccgcctc ggcaagcgtc
     1561 gccggctgga agttgccggc ggcatcgaag cgaagtggag ggggatcgcg gctacagccg
     1621 accaagctcg ctactgcgag cacagcccag ccgaagctca acgacgatcg ctgcacccgc
     1681 atctcgctcc ccgtttggcc ggcagggccg gccctagcga ttggttacat gatacgacgg
     1741 gaacctgaac ggcagcttga ggtttttctt agcggtcgta gaaccgcgac gacttctcgt
     1801 tttcgttgca gatgaactcg atgagctcgg cgtcggcgaa caaacgctgg ttcacgcgaa
     1861 ccgtgaacgg tttagtgtaa gccgccggat cttcgacagt tatgtcaatc tgcagcgtcc
     1921 cgtaattggg gcggcggtag cgctccgtca gcttgccggc gcccgtcagc gggctgccct
     1981 gcacgtcgag ccagacgtcg tcgcggaagc cgaccgtttc gacgacgagc gtgtcgcctt
     2041 cccaatgccc cgtcgaatac ccgtaccacc agggctgcaa ctcgggacct gctttcggca
     2101 gcgcccgacc gtccgtgaag atctgacgca agccctcgtt gccttcgtac atgatgacga
     2161 tgaggcccgg cgtctgaacg atcttgcgcg gctgcgggtg cagatgcagc tgcatgagcc
     2221 ccatcggcaa gcacaacgcg tcgggattgt tcttctgatt gtcggccatg cgctcgtcgc
     2281 gcagcatcgc ggcgagcggc gtgtagggca ggccgcctcg caagccggcg cccgtgttcc
     2341 agaacgtcgc ggccggcggg ccgccgtcgg ccgattgcag cgcgtcgaag tccggatttt
     2401 ggccgccggg accaaaacgc tcccagagtc ccgatagatc cggatggccg tcggcggcgc
     2461 gcggcgccgc agcgttgacg tcgacctt
//
LOCUS       DATLEE010000389         2739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1924863, whole genome shotgun sequence.
ACCESSION   DATLEE010000389 DATLEE010000000
VERSION     DATLEE010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924863"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="VM692_08330"
     CDS             complement(<1..1287)
                     /locus_tag="VM692_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ52216.1"
                     /translation="MTSNYRAADIEVLSGLEPVRRRPGMYTDTSRPNHLAHEVIDNSV
                     DEAVAGHAKKIDVTLFADGSLQVADDGRGMPVDIHPQEKLSGVELILTRLHAGGKFSN
                     KNYQFSGGLHGVGVSVVNALSKNLEVWIRRDGKEYNMSFADGERRSKLEVVGKVAKTT
                     TGTTVRFWPNPKYFDAPQISLPRLKHVLRAKAVLCPGLEVTLEVEKPKESERWVYTGG
                     LEQYLKEALGNGDFVPEQPFAGKLEGKLEAVEWAVVWRRDTGAEAVLESYVNLIPTPQ
                     GGTHVNGMRAGLTDALREFCEFRNLLPRGIKLAPEDVWEGVNFVLSVKIQEPQFSGQT
                     KERLSSRECATLVGGLLKDHFALWLNQHPETGDAIAQFAIENAQHRLKAAKTIVRKRV
                     VAGPALPGKLADCTLQDPAHTELFLVEGDSAGGSAKQ"
     gene            complement(1419..2000)
                     /locus_tag="VM692_08335"
     CDS             complement(1419..2000)
                     /locus_tag="VM692_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HUQ52217.1"
                     /translation="MDKLTTEDLLSLERYHKERPRLRAEVLAHKRHRQAPLGPHLTLY
                     FEDRITMRYQIQEMLRAERIFESEAIAEELEAYNPLIPDGSNLKATMMLEYPDVEQRR
                     RALEQLKGIENAVYLEVGTLGRVFAYADEDLERSDDTKTSAVHFLRFELDAGMCSALK
                     SGAPLTIGVDHESYRHELASAPEVRQSLAADLD"
     gene            complement(2020..>2739)
                     /locus_tag="VM692_08340"
     CDS             complement(2020..>2739)
                     /locus_tag="VM692_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019023824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HUQ52218.1"
                     /translation="GRVAVFTTCYGNRNHPKLVEDLVAVLEHNEIGVALLGKERCCGM
                     PKLELGDLRSIEKLKDANIDELDRYARDGWDLTAVVPSCVLMFKQELPLLFPEDERVK
                     RVAEAFFDPFEYLMLRHREGLLRTNFAASLGKISYQVPCHLRVQNVGLKTRELLQLVP
                     DTAVQAIERCSGHDGTYGVKKEYAAVSRKIARPVARQVDNAAADHFTSDCPMAAEQIA
                     TVAESAEPTHPIQLLRKAYGI"
BASE COUNT          439 a          896 c          873 g          531 t
ORIGIN      
        1 ctgcttcgcc gagcctccgg ccgaatcgcc ttcgacgaga aacaattcgg tatgcgctgg
       61 gtcttgcagc gtgcaatcgg cgagcttgcc gggtagcgcc ggccccgcga cgacgcgctt
      121 acgcactatg gttttggcgg ccttgaggcg atgttgcgcg ttctcgatcg cgaactgcgc
      181 gatcgcgtcg ccggtctcgg gatgctgatt cagccacagc gcgaaatggt ccttgaggag
      241 cccgcccacg agtgtggcgc actcgcgcga cgagagccgt tctttggtct gccccgagaa
      301 ctgcggctct tggatcttga cggacagcac gaaattgacg ccttcccaga cgtcctcggg
      361 ggcgagcttg atgccgcgcg gcagcaaatt gcggaactcg caaaactcgc gcagcgcatc
      421 ggtcaagcct gcgcgcatgc cgttgacgtg cgtgccgcct tgcggcgtcg gaatcaggtt
      481 gacgtagctc tcgagcacgg cttcagcgcc ggtatcgcgc cgccagacga cggcccattc
      541 gacggcctcg agcttgccct cgagcttgcc cgcgaacggc tgctcgggca cgaaatcacc
      601 gttgccgagc gcctccttga ggtattgctc gagcccgccc gtgtagaccc agcgctcgga
      661 ctccttcggc ttctcgacct cgagcgtgac ctcgagcccg ggacaaagca cggctttggc
      721 gcgcagcacg tgcttcaagc gcggcagcga gatttgcggc gcgtcgaagt acttcggatt
      781 cggccagaat cggaccgtcg tgcccgtcgt cgtcttcgcg accttgccga cgacctcgag
      841 cttgctgcgg cgctcgccgt cggcaaagct catgttgtat tccttgccgt cgcgccgaat
      901 ccaaacctcg agattcttcg acagcgcgtt gacgacggac acgcccacgc cgtgcaaacc
      961 gcccgagaac tgatagttct tgttcgaaaa cttgccgccc gcatgcaggc gcgtgaggat
     1021 cagctcgacg ccggacaatt tttcctgcgg gtggatgtcg accggcatgc cgcggccgtc
     1081 gtcggcgact tgcagcgacc cgtccgcgaa caacgtcacg tcgatcttct tggcgtggcc
     1141 cgcgacggcc tcgtcgacgc tgttgtcgat gacctcgtga gccaagtgat tcggccgcga
     1201 cgtgtccgtg tacatgccgg gacgacggcg cacgggctcc agcccgctca agacctcgat
     1261 gtcggcagcg cgataattgc tagtcattga tccggcctaa cactcgagca caagacccga
     1321 gttcccacgt aggccggcgc gattgtaacg tccggccgcg cgcgcgtcag cggtcttgcg
     1381 gattgtcgtt ccgtggacgc gcgtgagggg ggcaaccgct agtcgaggtc cgctgccagt
     1441 gactggcgca cctccggcgc gctcgcgagc tcgtggcggt agctctcgtg atccacgccg
     1501 atcgtcagcg gggcgccact cttcagggcg ctgcacatcc cggcgtcgag ctcgaaccgc
     1561 aggaaatgca cggccgaggt tttcgtgtcg tcgctgcgct cgaggtcctc gtcggcgtag
     1621 gcgaatacgc ggccgagcgt gccgacctcg agatagacgg cgttctcgat tcctttgagc
     1681 tgctcgagcg ccctgcggcg ctgctcgacg tcgggatatt cgagcatcat cgtggctttg
     1741 agattgctgc cgtccgggat cagcgggttg tatgcctcga gctcctcggc tatcgcttcg
     1801 gactcgaaga tgcgctcggc gcgcagcatc tcctgaatct gatagcgcat cgtgatgcga
     1861 tcctcgaaat acagcgtcag atgcggcccg agcggcgcct gacgatggcg cttgtgcgcg
     1921 agcacctcgg cacgcagccg cggccgttcc ttgtgatagc gctcgagcga cagcagatcc
     1981 tcggtcgtga gcttatccat ggcgtctgct ttccgttggc tagatgccgt acgccttgcg
     2041 taacagttga atcgggtgcg tcggctctgc cgattcggct acggttgcga tctgctcggc
     2101 cgccatcggg caatcgctcg tgaagtggtc ggccgcggcg ttgtcgacct ggcgggcgac
     2161 ggggcgcgcg atcttgcgcg acacggccgc gtattccttc ttcacgccgt acgtgccgtc
     2221 gtgaccggag cagcgctcga tggcctgcac ggcggtatcc ggaaccagct gcaagagctc
     2281 gcgagtcttc agcccgacgt tctgcacgcg caggtggcac ggcacttggt agctgatctt
     2341 gccgaggctc gcagcgaaat tcgtccgcag caggccttcg cgatgccgca gcatcaagta
     2401 ctcgaacgga tcgaagaacg cctcggcgac tcgcttgacg cgctcgtcct cggggaacag
     2461 cagcggcagc tcctgcttga acatcagcac gcacgacggg acgaccgcgg tcagatccca
     2521 gccgtcgcgc gcgtagcggt cgagctcgtc tatgttggcg tctttgagct tctcgatcga
     2581 ccgcaaatcg ccgagctcga gcttcggcat tccgcaacag cgctccttgc cgagcagcgc
     2641 cacgccgatc tcgttgtgct cgagcaccgc gacgaggtcc tcgacgagct tagggtgatt
     2701 gcgattgccg taacacgtcg tgaacaccgc gacgcgccc
//
LOCUS       DATLEE010000390         3243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10655313, whole genome shotgun sequence.
ACCESSION   DATLEE010000390 DATLEE010000000
VERSION     DATLEE010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10655313"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2363
                     /locus_tag="VM692_08345"
     CDS             <1..2363
                     /locus_tag="VM692_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ52219.1"
                     /translation="RHDAPATAAEQWPLFAQYCSDCHNADDFTAEIAFERLSPEAIPH
                     EPEIFEAVVRKLRGAQMPPPGAPQPDNATRRAWITSLESTLDAAAETAPNPGQIALHR
                     LNRTEYANAIGDLLALDIDVKALLPKDDESDGFDNVANVLKVSPSFLEQYIAAARVIS
                     EMAVGNPSAKRDSRVYYAEPGINQSFHVAGMPLGTRGGMLVEHFFPADGDYEFDIGGL
                     ARARYVEGLEYRHRLILAIDGKKVFENEIGGPDDVEAVDLRQAAAVAEINGRFENIRV
                     PLTAGPHTIAATFVARTMAESDAVLQPFVPGGGEVGIIEGEESPLKIQRLEINGPFTA
                     TGLSDTPSRQRIFTCRPQDPADEPKCAREIVTRLTRAAFRRPVTDDELKTPLEFYEAG
                     KARGGFEAGVRNALMIVLASPEFLYRFTAPPDDSAPGSIYAVDQYGLASRLSFFLWSS
                     VPDDELLGFAERGELGDPRVIDAQVKRMLRDPRAASLVTNFAQQWLDIRGVRDIVPDP
                     VLFPEYNPDLGDAFAKELELFVGSVFLEDRSVLELLNGKSTYLNERLALHYGVAGVRG
                     DSFRKVELADRNRWGLFGKGGILMATSYPNRTAPVLRGAWILEAITGTPPASPPPNVE
                     AFPETQEGEQPKTVRERLEQHRSNPSCNGCHGVMDPLGFALENFDAIGAWRVKDREAL
                     AAIDSSGQLADGTAVNGPADLRDALLADPTQFVQTLTEKLMIYALGRSVEYYDMPAVR
                     RIVRDAKREDYRFSAILAGIAQSEPFRFSKAPAADDDSVTAQASEN"
     gene            2422..>3243
                     /locus_tag="VM692_08350"
     CDS             2422..>3243
                     /locus_tag="VM692_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ52220.1"
                     /translation="MFVTKKHLSRRTVLRGLGVTVALPLLDAMIPARTALAHTAAGPN
                     PKLGFVYFPHGAIMSKWTPSGEGKSFELSPLLEPLAPFKDQLTIVSGLENKHAAGPTH
                     AITPGTWLSGVTPRISHDPYGGVTADQVAAQHIGQDTPLPSLEVATEEAVGGGACDRN
                     YGCSYGATISFRTPSTPLPMEFNPRKLFQRLFGQGDTPEEREVLAAQYASILDVVADK
                     ASALQKVVGPSDRRMLSDYLESVREIERRVQKMEANDLSHISLPDAPVGIPSSFDE"
BASE COUNT          547 a         1097 c         1104 g          495 t
ORIGIN      
        1 cacggcacga tgcgccggcg acggccgccg agcagtggcc gctgttcgct cagtactgca
       61 gcgattgtca caacgccgac gacttcacgg cggagatcgc gttcgagcgc ttgtcgcccg
      121 aggccatccc gcacgagccc gagatcttcg aagccgtcgt acgcaagctg cgcggcgcgc
      181 agatgccgcc gccgggtgcg ccgcaacccg acaatgcgac gcgccgcgcg tggatcacga
      241 gcctcgagag cacgctcgac gcggccgcgg agaccgcgcc gaacccgggc cagatcgcgc
      301 tgcaccgctt gaatcgcact gaatacgcca acgcgatcgg cgacttgctc gcgctcgaca
      361 tcgacgtcaa ggcgctgctg ccgaaggacg acgagagcga cggcttcgac aacgtcgcca
      421 acgtgctcaa ggtgagcccg tcgttcctcg agcagtacat cgccgccgcg cgcgtcatca
      481 gcgagatggc cgtcggcaat ccgagtgcga agcgcgacag ccgcgtctac tacgccgagc
      541 ccggcatcaa tcagagcttc cacgtcgccg gcatgccgct cggcacgcgc ggcggcatgc
      601 tagtcgagca tttttttccg gccgacggcg actacgagtt cgacatcggc ggcctcgcgc
      661 gggcgcggta cgtcgagggc ctcgagtatc ggcatcggct catcctcgcg atcgacggca
      721 agaaagtgtt cgagaacgag atcggcgggc ccgacgatgt cgaggccgtc gacctgcgcc
      781 aggccgccgc cgtcgccgag atcaacggcc gcttcgagaa catccgtgtg ccgctgacgg
      841 cggggccgca cacgatcgcg gcgacgttcg tggcgcgcac gatggcggaa tccgacgccg
      901 tgttgcagcc gttcgtgccc ggcggcggcg aggtcggcat catcgaaggc gaggagagcc
      961 cgctgaagat tcagcggctc gagatcaacg gcccgttcac ggcgacaggc ttgagcgaca
     1021 cgccaagccg tcagcgcatc ttcacgtgcc ggccccagga cccggccgac gagccgaagt
     1081 gcgcgcgcga gatcgtcacg cgcttgacgc gcgcggcgtt ccgccggccc gtgacggatg
     1141 acgagctgaa gacgccgctc gagttctacg aggccggcaa agctcgaggc ggcttcgagg
     1201 cgggcgtgcg caacgcgctg atgatcgtgc tcgcgtcgcc ggagttcctg tatcggttca
     1261 cggcgccgcc cgacgattcg gcgccgggct cgatctacgc cgtcgatcag tacggcctcg
     1321 cgtcgcggct gtcgttcttc ctctggagca gcgtgcccga cgacgagttg ctcggctttg
     1381 ccgagcgcgg cgagctcggc gatccgcgcg tcatcgacgc ccaggtcaag cgcatgctgc
     1441 gcgatccgcg cgcggcgtcg ctcgtgacga acttcgcgca gcagtggctc gacatccgcg
     1501 gcgtgcgcga catcgtgccc gatcccgtgc tgttccccga gtacaacccc gacttgggcg
     1561 acgcgttcgc gaaggagctc gagctcttcg tcggcagcgt gttcctcgag gaccgcagcg
     1621 tgctcgagct gctgaacggc aagagcacgt acctgaacga gcggctcgcc ttgcattacg
     1681 gcgtcgcggg cgtgcgcggc gacagcttcc gcaaggtcga gctggccgac cggaaccgct
     1741 ggggcttgtt cggcaagggt ggcattctga tggcgacgtc gtatccgaac cgcacggcgc
     1801 ccgtgctgcg cggcgcgtgg atcctcgagg cgatcacggg cacgccgccc gcgtcgccgc
     1861 cgccgaacgt cgaggccttc cccgagacgc aagagggcga gcagccgaag accgtgcgcg
     1921 agcggctcga gcagcatcgc tcgaatccga gctgcaacgg ttgccacggc gtcatggatc
     1981 cactcggctt cgcgctggag aatttcgacg cgatcggtgc gtggcgtgtg aaggatcgcg
     2041 aggctcttgc cgcgatcgat tcgtcgggcc agcttgccga cggcactgca gtcaacggcc
     2101 ccgccgattt gcgggatgcg ctgctcgcgg acccgacgca gttcgtgcaa acgttgaccg
     2161 agaagctcat gatttacgcg ctcggccgca gcgtcgagta ctacgacatg ccggccgtgc
     2221 ggcgcatcgt gcgcgacgcc aaacgcgagg actatcggtt ctcggcgatc ttggcgggca
     2281 tcgcgcagag tgagccgttt agattcagca aggcgcccgc ggcggacgac gatagcgtca
     2341 ccgcccaggc gtccgagaat tgacgcgggc agggatgcac gcgctagagc aacgcaggag
     2401 cacttgccga ggagagccat gatgttcgtg accaagaagc atctgtcgcg gcgcacggtg
     2461 ttgcgcgggc tcggcgtgac cgtagcgctg ccgttgctcg atgcgatgat tccggctcgc
     2521 acggccctcg cgcacaccgc cgcgggcccg aacccgaagc ttggcttcgt gtacttcccg
     2581 cacggcgcga tcatgagcaa gtggacacct tcgggtgagg gcaagagctt cgagctttcg
     2641 ccgctgctcg agccgctggc accgttcaaa gatcaactga cgatcgtcag cggcctcgag
     2701 aacaagcacg ccgccgggcc cacgcatgcg atcacgccgg gcacgtggct ctcgggcgtc
     2761 acgccgcgca tcagtcacga cccgtacggc ggcgtgacgg cggaccaagt cgccgcgcag
     2821 cacatcggcc aggacacgcc gctgccgtcg ctcgaggtgg cgaccgaaga agctgtcggc
     2881 ggtggcgcct gcgaccgtaa ctacggctgc agctatggcg cgacgatctc gttccgcacg
     2941 ccgtcgacgc cgttgccgat ggagttcaac ccgcgcaagt tgttccagcg cttgttcggc
     3001 caaggcgaca cgcccgagga acgcgaggtg ctcgcggcgc aatacgccag cattctcgac
     3061 gtcgtcgcgg acaaggcctc tgcgctgcag aaggtcgtcg gcccgagcga ccgccggatg
     3121 ctgagcgact atctcgagag cgtgcgcgag atcgagcggc gcgtgcagaa gatggaggcc
     3181 aacgacctct cgcatatctc gttgcccgac gcgcccgtcg gcattccgtc gagcttcgac
     3241 gag
//
LOCUS       DATLEE010000391         4414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_440004, whole genome shotgun sequence.
ACCESSION   DATLEE010000391 DATLEE010000000
VERSION     DATLEE010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440004"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VM692_08355"
     CDS             complement(<1..411)
                     /locus_tag="VM692_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ52221.1"
                     /translation="MILKRSRCAGFRASICRSSGRPIRSRPCSVAPESFTLARLRLRR
                     PNLKDADAMFAIGADPEVAHFADWPLSTERGAALERLRQRAEQWDAGVEYYWIITLAH
                     DDRAIGAISTCVDRDAADVGYLLDRRQWGHGYATE"
     gene            complement(314..760)
                     /locus_tag="VM692_08360"
     CDS             complement(314..760)
                     /locus_tag="VM692_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HUQ52222.1"
                     /translation="MSPYFRQLRAKIGHDLLLLPAVAAVIRDSDGRLLLQERSAGDGW
                     SLPAGLIEPGESPEQCLRREVLEETGFVVVPTRIIGVFGGEGFRHVYPNGDVVEATVV
                     LYRCVVVGEEPGGPSDPETKSLRWFPRVDMPKLGAAYPLEALFGGA"
     gene            complement(760..1128)
                     /locus_tag="VM692_08365"
     CDS             complement(760..1128)
                     /locus_tag="VM692_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52223.1"
                     /translation="MRSRRHSTTIAAIAMTMLSSACGAQGLSLIDHCANDVEPIDTVL
                     PLKGAHTYRVTARFVIDVNGGVVAPSVKASQLVMASGATVPVPPTVDRSFLDALRKWR
                     YARRPKPCLRTVDLEMLLSV"
     gene            complement(1149..1538)
                     /locus_tag="VM692_08370"
     CDS             complement(1149..1538)
                     /locus_tag="VM692_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52224.1"
                     /translation="MSRRLILVCTIVFGIIPATLSLLANALYYLRGMTEIPEGAAIVV
                     EWGEVLMLCVVAVAGVVGYVSLFFAARGRVGGGVVIGLLLGVAALSYAIALGFTPLWS
                     GSPVIVGLAHVARYFTRQRARRGRGFA"
     gene            complement(1766..2878)
                     /locus_tag="VM692_08375"
     CDS             complement(1766..2878)
                     /locus_tag="VM692_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HUQ52225.1"
                     /translation="MKAFVSALVLAYVGGALPSAAQSAATPAATRPLPLPPGLAGVWR
                     VNGRKVADDASSWSLAAFSTDGKVVGVADEGGTRVYRASDGQLLRMVPAPFSTGQFAY
                     SLAISASGLLALGRVGGLDVFDLSSSAEPLKYHCAGICGPVSALAFSPNGSLLAYQAA
                     LSAAEAKTGLVNVVDLRQNARVAELEASATRTGVMFAADGRTLLAANVTRVDGTGTFG
                     LRGWSGGANWRRVRDEAGAQVPAGSIGPYAFDERVAAYALDGRLELREHANGALAWSV
                     PLVPPGLDAVVDGAPMRLELVAFASRGDLVLSYETPASGAAPGTLVFRRMQDGATIAM
                     YDVAGVSSIAVAPDGASFMYSTGAGRTYTVLARVPR"
     gene            complement(2875..3669)
                     /gene="thyA"
                     /locus_tag="VM692_08380"
     CDS             complement(2875..3669)
                     /gene="thyA"
                     /locus_tag="VM692_08380"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HUQ52226.1"
                     /translation="MRQYLDLLRAIKERGVRKGDRTGTGTLSLFGYQMRFDLDAGFPV
                     LTTKKLHLRSIIHELLWFLKGDTNVQYLRDNEVTIWDEWADEKGNLGPVYGAQWRTWR
                     TADGQHVDQIQRALDLIRTDPNSRRIIVSAWNVGELEEMALAPCHALFQFHVAQGRLS
                     CQLYQRSADVFLGVPFNIASYALLLTMMAQQTNLRPGELIWTGGDCHLYLNHMVQADL
                     QLTREPLPLPTLAIKRKPPSLFDYKFEDFELLDYYAHGSIKAPIAV"
     gene            complement(3635..>4414)
                     /gene="lgt"
                     /locus_tag="VM692_08385"
     CDS             complement(3635..>4414)
                     /gene="lgt"
                     /locus_tag="VM692_08385"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HUQ52227.1"
                     /translation="GPLAVRWYGIMYLIGFVGGWLGARVRAKQPWSRVKPEQVDDIVF
                     YVALGAIAGGRIGYMFIYGLSELLANPLSILKVWEGGMSFHGGFVGVLVAMWLYSRAI
                     KQPFLAIMDFVAPWTAIGLGAGRIGNFINGELWGKPTSPDAPWAVIVDGQARHPSQLY
                     EFFLEGVVLFLVLWLYTKKPRPFMASSALFLLVYGVSRFIIEFVREPDQQIGYLALGW
                     VTMGQVLSAPMIIAGVILLVLAYKRDTKALDAAIPGPSAGH"
BASE COUNT          818 a         1515 c         1423 g          658 t
ORIGIN      
        1 ctccgtcgcg tagccatggc cccattgccg gcgatcgagt aaatagccga cgtccgccgc
       61 atcccgatcc acgcacgtcg agatggcgcc gatcgcccga tcgtcgtgcg ccagagtgat
      121 gatccagtag tactcgacgc cggcgtccca ctgctcagcc cgttgccgca gccgctcgag
      181 cgccgcacct cgttcggtgc tcaacggcca gtcggcgaag tgcgcgacct cggggtcggc
      241 gccgatcgcg aacatcgcgt cggcgtcctt aaggttaggc cggcgcaggc gcagccgagc
      301 gagcgtgaag ctttcaggcg ccaccgaaca gggcctcgag cggataggcc gccccgagct
      361 tcggcatatc gacgcgcgga aaccagcgca gcgacttcgt ttcaggatca ctcggtccgc
      421 cgggctcttc gccgacgacg acgcagcgat acagcacgac cgtagcctcg acgacgtcgc
      481 cgttcgggta gacgtgacga aaaccttcgc cgccgaacac gcctatgatt cgcgtcggca
      541 ccacgacgaa cccggtctct tcgagcacct cgcgccgaag gcattgctcg ggactctcgc
      601 cgggctcgat caaaccggcg ggcagactcc aaccgtcgcc cgcgctccgt tcctgcagca
      661 gtaggcgccc gtcgctgtcg cgaatgacgg ctgcaacggc cggtagcagc agaagatcgt
      721 gaccgatctt ggcgcgcagc tgacgaaagt acggcgacat cagacggaca gcagcatttc
      781 caggtcgacg gttcgcaggc acggcttcgg gcggcgggcg taccgccatt ttctgagggc
      841 atcgagaaac gatctgtcga cggtcggcgg caccggcacc gtggcgccgc tagccatgac
      901 gagctgcgac gccttgacgc tcggagcgac gacgccgccg ttaacgtcga tgacgaagcg
      961 cgccgtgacg cgatacgtat gcgcgccttt gagcggcagc accgtgtcga tcggctcgac
     1021 gtcgttcgcg cagtggtcga tgaggctcaa gccctgcgcg ccgcaggcgg agctcagcat
     1081 cgtcattgcg atggcggcaa tcgtcgtgct gtgtcttcgt gatctcatgg gcgtcagctc
     1141 ctccggattc acgcgaagcc gcggcctcgc cgagcgcgct gacgcgtgaa atagcgggcg
     1201 acgtgcgcca agccgacgat gacgggcgaa cccgaccaaa gcggcgtgaa tcccaacgcg
     1261 atcgcatacg acagcgcggc gactccgagc agcaagccga tgacgacgcc gccaccgacg
     1321 cgcccgcgcg cggcgaagaa cagcgacacg tagccgacga cgccggcgac ggcgacgacg
     1381 cacagcatca gcacttcgcc ccactcgacg acgattgcgg caccttcagg gatttcggtc
     1441 atgcctctca ggtagtaaag cgcattcgcc agcaacgaca acgtcgccgg aatgatgccg
     1501 aagacgatcg tgcaaactaa aattaggcga cgcgacatgg ctatggcggg gcctcttcga
     1561 cattcacccg agtacgttgc aactgactcg cgaattcctt gttagcaggg aacaggtaat
     1621 actccccgtt gccaatgttc gccaagagag ctgctaagcc gagcttggag atcgattccc
     1681 tagtcacgca caacgcaacg atcgaagtgg ccaatttcat gcgaagccgg ctcctagcat
     1741 gacagcagct cagcggcagc tccgctcacc gcgggacccg cgccaacacc gtgtaggtcc
     1801 gccctgctcc cgtgctgtac atgaagctcg cgccgtccgg cgcgacagcg atggagctga
     1861 cgccggcgac atcgtacatc gcgatcgtcg caccgtcctg catgcggcgg aacaccagcg
     1921 tgcccggcgc ggcgcctgag gccggggttt catagctcag cacgagatcg ccgcggctcg
     1981 cgaacgccac gagctcgagc cgcatcggcg ccccgtcgac gacggcatcg aggcccggcg
     2041 gcacgagcgg cacggaccac gcgagcgcgc cgttcgcgtg ctcgcgcagc tcgaggcggc
     2101 cgtcgagtgc gtacgccgcc acccgttcgt cgaaggcata cggcccgatc gatcccgcgg
     2161 gcacttgcgc gccggcctcg tcgcgcacgc ggcgccagtt cgccccgccg ctccaaccgc
     2221 gcagcccgaa cgtgccggtg ccgtcgacgc gcgtgacgtt ggccgcgagc agcgtgcggc
     2281 cgtccgctgc gaacatcacg cccgtgcgcg tcgcggacgc ttcgagctcg gcgacgcgcg
     2341 cgttttgccg caagtcgacg acgttgacga gccccgtctt cgcctccgcg gcgctcagcg
     2401 cggcctggta ggcgagcaac gagccgttcg gcgagaacgc cagcgccgat acgggaccgc
     2461 aaatgccggc gcagtgatac ttcagtggct ccgcgctcga cgacaggtcg aagacgtcga
     2521 ggccaccgac gcggcccagc gcgagcagcc cgctcgccga gatcgcgagc gagtatgcga
     2581 actggcccgt cgagaacggt gcgggaacca tgcgcagcaa ctgcccgtcg ctcgcgcggt
     2641 agacgcgcgt gccgccctcg tccgcgacgc cgacgacctt accgtcggtc gagaacgcgg
     2701 ccagcgacca cgacgatgca tcgtcggcga ccttgcggcc gttcacgcgc cagacgcccg
     2761 cgagccccgg cggcagcggc aacgggcgcg tcgcggccgg cgtcgccgcg gactgcgccg
     2821 cgctcggtag cgcgccgccg acataggcca gcaccaaggc ggagacgaaa gccttcacac
     2881 cgcgatcggc gctttgatcg agccgtgcgc gtaatagtcg agcagctcga aatcctcgaa
     2941 cttgtagtcg aacaagctag gaggtttgcg cttgatggcc aacgtcggca gcggcagcgg
     3001 ctcgcgcgtc agctgcaggt ccgcctgcac catgtgattc aagtacagat ggcaatcgcc
     3061 gcccgtccag atcagctcgc cgggccgcag gttcgtttgc tgcgccatca tcgtcagcag
     3121 cagcgcgtac gacgcgatgt tgaacggcac gccgaggaac acgtcggcgc tgcgctgata
     3181 gagctggcac gagagccgcc cctgcgcgac gtgaaattgg aacagcgcgt gacacggcgc
     3241 gagcgccatc tcctcgagct cgccgacgtt ccaggcgctg acgatgatgc ggcgcgagtt
     3301 cgggtccgtg cggatcaagt cgagcgcccg ctggatctgg tcgacgtgct ggccgtcggc
     3361 cgtgcgccac gtgcgccact gcgcgccata gacgggtccc aagttgcctt tctcgtcggc
     3421 ccactcgtcc cagatcgtga cttcgttgtc gcgcaggtac tggacgttgg tgtcgccctt
     3481 caggaaccac agcagctcgt gaatgatcga gcgcagatgc agcttcttcg tcgtgagcac
     3541 gggaaagccc gcgtcgaggt cgaagcgcat ctgatagccg aacaggctca gcgtgccggt
     3601 gcccgtgcga tcgcccttgc gaacgccgcg ctctttaatg gcccgcagaa ggtccaggta
     3661 ttgccgcatc taacgccttg gtgtcgcgct tgtaagcgag caccagcaaa atgacacctg
     3721 cgatgatcat cggcgccgaa agcacctgac ccatcgtcac ccaaccgagc gcgagatagc
     3781 cgatctgctg gtccggctcg cggacgaact cgatgatgaa acgcgagacg ccgtacacga
     3841 gcaggaacaa cgccgagctt gccatgaacg gccgcggctt cttcgtgtag agccacaaaa
     3901 cgaggaacag cacgacgccc tcgaggaaga actcgtagag ctggctcggg tggcgcgcct
     3961 gcccgtccac gatgacggcc cacggcgcgt cggggctcgt cggcttgccc cagagctcgc
     4021 cgttgatgaa attgccgatg cggccggcgc cgagcccgat cgccgtccag ggcgcgacga
     4081 aatccatgat ggccaagaac ggctgcttga tggcgcggct gtagagccac atcgccacga
     4141 gcacgccgac gaacccgccg tgaaacgaca tgccgccttc ccagaccttg aggatgctga
     4201 gcggattcgc gagcagctcg ctcaagccgt aaatgaacat gtagccgatg cggccgccgg
     4261 cgatcgcgcc gagcgcgacg tagaacacga tgtcgtcgac ctgctcgggc ttgacgcggc
     4321 tccagggctg tttcgcgcgc acgcgcgcgc ccagccagcc cccgacgaag ccgatgaggt
     4381 acatgattcc gtaccagcgc acggcaagcg ggcc
//
LOCUS       DATLEE010000392         5996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3212040, whole genome shotgun sequence.
ACCESSION   DATLEE010000392 DATLEE010000000
VERSION     DATLEE010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3212040"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VM692_08390"
     CDS             <1..296
                     /locus_tag="VM692_08390"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HUQ52228.1"
                     /translation="SLFSVCVLTGWGATWLALRERGLRGVIVVADSDIHYRKAVEGEL
                     VCRCAPDADALRAGLEQFAASGRASFELNCAIDRDDKHAVTFTGKYIVNAQHS"
     gene            complement(293..925)
                     /locus_tag="VM692_08395"
     CDS             complement(293..925)
                     /locus_tag="VM692_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095298.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HUQ52229.1"
                     /translation="MDFVSPAELVELVEVVFVNLVLSADNAIVVGIAVAGLPAKNRLR
                     IMAIGIAGATLLRVVFAIFTAQLLEVVGLKLAGGALLLWICWKLWTELRTGRLIEPRQ
                     QGPDDQSEPSTTGARPKTMRQAMTQIIVADVSMSLDNVLAVAGVAREHLWVLVSGLVL
                     SIAFMGLAAAVIARLLSRFQWIAYVGLAIILYVALRMIFDGAVELFEAAA"
     gene            1089..3170
                     /locus_tag="VM692_08400"
     CDS             1089..3170
                     /locus_tag="VM692_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ52230.1"
                     /translation="MHSLRWHHGLACCAAPTLLLGSAALAQQPAQGPLEEIVVRATMI
                     ERTLDRVPAAVSVIDEAAIQNGRQQLGLDEALSRVPGLFMQNRYNFAQDLRVSIRGFG
                     ARAQFGIRGIKVLVDGIPETLPDGQGSVDSIDLGATSQIEVIRGPSSSLYGNASGGVI
                     SLISEGGRAEPYAQVRVAAGGYGYEKAQFKFGGETDRLNYLVSLSNQELDGYRDRSAY
                     ENKLLTGRFDLDLGKDRSLLTVLNLTDQPISDDPGGLTAAVVPVNPRSAAPLNTQFLA
                     GESLEQQRLGFVYTTRAGEHGTIRARNYYAWRDFGNLLPTLLQGIVDLDRKFAGGGVS
                     YSYDGFWLDRPNRLVAGADFDDQDDDRRRYNNLNGLQGPLGFDQNEHVTSHGVFLQNE
                     LSISENVQLSFGVRFDEVEFEITDRFLGDGRDDSGSKSFDDTSPMVGLVVQLRDDLSF
                     YTTYSSAFETPTTTEFNRQDGLGGFNQALEPQLARNFEVGLRGSVDDTQRYEIALFTI
                     KVDDELIGREIPTSPGRNFFENAGETSRKGLELSWMANPTDRINATVSYTYSDFQFDR
                     FNETVGTQTFNRHGLIIPGTAENTVFGEFEYRAPRGWYGAIDAMYVDEQFGDNGNLVV
                     IPSYTLTNLRFGYDAEIGKLTLSPFLGINNAGDQPYTSNVRLNAAAARYFEPGPLRNA
                     YAGITVDWKFR"
     gene            3155..4063
                     /locus_tag="VM692_08405"
     CDS             3155..4063
                     /locus_tag="VM692_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUQ52231.1"
                     /translation="MEVPVDDAADRALPAAHDAAAARTRDVATGVYYDRLSRWTARVS
                     GLGYGGGRETLTAHRALADPRASGRPTFTRLHDVLADALPSPLRGHVLDAGCGLGGTM
                     LDLAARGPATFVGITLSAQQAAVARRAAAEAGLEARVDVRVGSYDEPPAGPFDAVIAI
                     ESLAHSAQPGATVAALAARLAPGGRLVVVDDMPTVEARGTYALASFQAGWRLPVLLSA
                     AELAAAFERCGLVPAGDRDLTCEVRPRTLRRIAQLETLNRALHRVLPSGGIRELLDSY
                     RGGLALERLYRRGLMNYRLVVARKPG"
     gene            complement(4060..5043)
                     /locus_tag="VM692_08410"
     CDS             complement(4060..5043)
                     /locus_tag="VM692_08410"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HUQ52232.1"
                     /translation="MSATTQKRWRRRPPPPRWLMAPIGALILAIYWPVTKLFAKRWPR
                     WVTRTITKLLTRFPQDYAPTAHDVLVCSYFKSGTNWTMQIALQIAYRGRAEFEHIHDL
                     VAWPDMNMRADYAIPLANEEPQRSAPTGLRIIKTHMALDALPYSPAARSICVVRDPKD
                     VFVSSYHFTRAMVLGPLMPSVEAWLDAYLSPDTTLGSWARHVHSGWRLRNKGNVLFLT
                     YESMRADLPATVDKIAAFMGVTLAPDERAAVIERSSFAYMKKIGHKFDAPGAPWATSA
                     GSMLRRGESGKSAELLSPEQQRRIDDYWRAELARIECDFPYDEQFAPPAAG"
     gene            complement(5082..>5996)
                     /locus_tag="VM692_08415"
     CDS             complement(5082..>5996)
                     /locus_tag="VM692_08415"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HUQ52233.1"
                     /translation="MPVLVGPKTDGQKFPGAEYTLCIEAMMQDGKALQAGTSHFLGQN
                     FAKAFDVTFQNEQGKREHAWATSWGVSTRLIGGLIMTHADDDGFVLPPKLAPQHVVII
                     PIYRSDDEKARVLDYCRKLAVELRAQRFNDAPVRALVDERDERGGEKVWHWVRRGVPL
                     RLEIGPRDIDKDAVFAARRDRAPKDKQSISRTEFILTIASTLQAIQTGLFEKAAAYRK
                     EHTRVIDDRDEFYKFFASPRVADDVPTPIHAGFALTHFSGDVDLEKKIKDDLSVTVRC
                     IPLDKSEAGTCPFTGKPSAQRVVWAKAY"
BASE COUNT         1011 a         2062 c         1938 g          985 t
ORIGIN      
        1 gcagcttgtt ctcggtctgc gtcttgacgg gctggggagc gacctggctc gcgttgcgcg
       61 aacgagggct caggggcgtc atcgtcgtcg ccgacagcga catccactac cgcaaagccg
      121 tcgagggcga gctcgtgtgc cgctgtgctc ccgacgccga cgcccttcgc gccgggctag
      181 agcaattcgc ggccagcggc cgcgcgagct tcgagctcaa ctgcgcgatc gatcgggacg
      241 acaaacacgc cgtgacgttc acgggtaagt acatcgtcaa cgcgcagcat tcctaagccg
      301 cggcctcgaa cagctcgacc gcgccgtcga agatcatgcg caacgcgacg tagagaatga
      361 tcgcgaggcc gacgtacgca atccactgaa accgggacaa caggcgcgcg atgacggcgg
      421 ccgcaaggcc catgaacgcg atcgacaaca ccaagccgga gaccagcacc cacaggtgct
      481 cgcgcgcgac acccgccacc gcgagcacat tgtcgagcga catcgagacg tcggccacga
      541 tgatttgcgt catggcctgt cgcatcgttt tcggccgtgc gcccgtagtt gacggctctg
      601 actgatcgtc cgggccctgt tggcgcggct cgatgagccg cccggtgcgc agctcggtcc
      661 acaacttcca gcagatccac agcaacagcg cgccgcctgc cagcttcaac ccgacgactt
      721 cgagcagttg cgccgtgaaa atcgcaaaca cgacgcgcag cagcgtggcg cccgcgatgc
      781 cgatcgccat gatgcgcagc cgatttttcg ccggtaggcc cgcgacggcg atccccacga
      841 cgatcgcgtt atcggcggac aagacgaggt tgacgaatac aacctcgacg agctccacca
      901 actcggccgg tgaaacgaag tccatacgag tggtcttggc tgcggaaagg gtccgccagt
      961 gtgcccgtaa gcgcgcgtca tttcacgcgc gttgttgcgg cgcaacgggc gcggcgagcg
     1021 ccgttgcccg accgagcggc ctcagtcatg atacccggcc atcacgcctg gcttcgaacg
     1081 ggattcccat gcactcactt cgctggcacc acggtctcgc ttgttgcgcg gccccgacct
     1141 tgcttctagg tagtgccgcg ctcgcgcagc agcctgcgca gggcccgctc gaggagatcg
     1201 tcgtgagggc cacgatgatc gaacgcacgc tcgaccgcgt acccgcggcc gtcagcgtca
     1261 tcgacgaagc ggcgattcag aacgggcgac agcagctagg cttggacgaa gcgctgagcc
     1321 gcgtgccggg cctattcatg cagaaccgct acaacttcgc gcaagacctt cgcgtgtcga
     1381 tccgcggctt cggcgctcgc gcgcaattcg ggattcgcgg tatcaaggtg ctcgtcgacg
     1441 gcatcccgga gacgctgccc gatggccaag gctccgtcga cagcatcgat ctcggcgcga
     1501 cgagccagat cgaagtcatc cgcggcccga gctcctcgct ctacggcaac gcgtccggcg
     1561 gcgtcatcag cttgatctcg gaaggcggcc gtgcggagcc gtatgcgcag gtgcgcgtgg
     1621 cggcgggcgg ttacgggtac gaaaaagctc aattcaagtt cggcggcgaa accgatcgcc
     1681 tcaactatct cgtcagcctc tccaatcagg agctcgacgg ttaccgcgac cgcagcgcct
     1741 acgagaacaa gctgctgacg ggccggttcg acctcgatct cggcaaagac cgaagtctgt
     1801 tgaccgtcct caatctgacc gatcaaccga tctcggacga tcccggcggc ttgacggcag
     1861 ccgtggttcc cgtgaacccg cgatccgctg cgccgctgaa cacgcagttc ctggccggcg
     1921 aatcgctcga gcagcagcgc ctcggcttcg tctacacgac gcgggccggc gagcacggca
     1981 cgattcgcgc gcgcaactac tacgcgtggc gcgatttcgg caacctgctg ccgacgctgt
     2041 tgcaaggcat cgtcgatctc gaccgcaagt tcgccggcgg cggcgtcagc tacagctacg
     2101 acggcttctg gctcgatcga ccgaatcgcc tcgtcgccgg agccgacttc gacgaccaag
     2161 acgacgaccg ccgccgctac aacaacttga acggcctgca aggcccgctc ggcttcgatc
     2221 agaacgagca cgtgacgagc cacggtgtct tcctgcagaa cgagctcagc atttccgaga
     2281 acgtgcagct ttcgttcggc gtgcgcttcg atgaagtcga attcgagatc acggatcgct
     2341 tcttgggcga cggccgcgac gactcgggct cgaaaagctt cgacgatacg agcccgatgg
     2401 tcggcctcgt cgtccagctc cgcgacgact tgagcttcta cacaacgtac tcgagcgcgt
     2461 tcgagacgcc gacgacgacg gagttcaacc gccaggacgg cctcggcggg ttcaaccaag
     2521 cgctcgagcc gcagctcgcg cgcaacttcg aggtcggcct gcgcggctcg gtcgacgaca
     2581 cgcagcgtta cgagatcgcg ctgttcacca taaaagtcga cgacgagctc atcggccgcg
     2641 agattccgac gtcgccgggc cgcaacttct tcgagaacgc cggcgagacg agccgcaaag
     2701 gcctcgagct ctcgtggatg gcaaacccca cggaccgcat caatgcgacg gtcagctaca
     2761 cctattcgga cttccagttc gaccgcttca acgagacggt cggcacgcag acgttcaatc
     2821 ggcacggcct catcattccc ggcacggccg agaacaccgt gttcggggag ttcgaatacc
     2881 gcgcgccgcg cggctggtac ggtgcaatcg acgcaatgta tgtcgacgag caattcggcg
     2941 acaacggcaa cttggtcgtg atcccgagct acacgctgac gaacttgcgc ttcggctacg
     3001 acgcagagat cggcaagctc acgctgtcgc cgttcttggg catcaacaac gccggcgacc
     3061 aaccttacac ttcgaacgtc cggttgaacg cggccgccgc gcgctacttc gagcccggcc
     3121 cgctgcgcaa cgcctacgcc ggcatcaccg tcgattggaa gttccggtag acgacgcggc
     3181 cgatcgtgca cttcccgcgg cgcacgacgc cgcggcggcg cgcacgcgtg acgtcgccac
     3241 aggcgtctac tacgaccgtc tgtcgcgctg gacggcccgc gtctctgggc tcggctacgg
     3301 gggcggccgc gaaaccctga cggcgcaccg ggcactggcc gacccgcgcg cgtcgggccg
     3361 gccgacgttc acgcggctgc acgacgtcct cgccgacgca ctgccctctc cgcttcgcgg
     3421 ccacgtgctc gacgccggct gcggcctcgg cggcacgatg ctcgacctcg ccgcgcgcgg
     3481 tcccgcgacg ttcgtcggta tcacgttgag tgcgcagcaa gcggccgtcg cgcgccgcgc
     3541 cgccgccgaa gccggactcg aagcgcgcgt cgacgtgcgc gtcggcagct acgacgagcc
     3601 gccggcaggg ccgttcgatg ccgtgatcgc gatcgagtca ctcgcgcatt ctgcgcaacc
     3661 gggcgcgacg gtggccgcgc tggcggcacg ccttgcgccg ggcggccggc tcgtcgtcgt
     3721 cgacgacatg ccgaccgtcg aagcgcgcgg cacttacgct ctcgcgtcgt tccaggccgg
     3781 ctggcgtctg cccgtgctgc tgtcggccgc ggagctcgcg gccgctttcg agcgctgcgg
     3841 cctcgtgccg gccggcgatc gagatctaac ctgcgaggtt cggccgcgaa ccctgcggcg
     3901 catcgcgcag ctcgaaacgt tgaaccgcgc gctgcatcgc gtcttgccgt cggggggcat
     3961 acgcgagctg ctcgattcct accgcggcgg cctcgcgctc gaacgcctgt accgccgcgg
     4021 tttgatgaac taccgcctcg tcgtcgcgcg caagcccggc tagcccgccg cgggcggcgc
     4081 gaactgctcg tcgtaaggga agtcgcactc gatgcgcgcg agctcggcgc gccaatagtc
     4141 gtcgatgcgc cgctgctgct ccggcgagag cagctcggcc gacttgccgc tctcaccgcg
     4201 gcgcaacatc gagcccgcgg acgtggccca cggcgcgccc ggcgcgtcga acttgtggcc
     4261 gatcttcttc atgtatgcga acgacgagcg ctcgatgacg gccgcgcgct cgtccggcgc
     4321 gagcgtgacg cccatgaacg cggcgatctt gtcgacggtc gccggcaggt ctgcccgcat
     4381 gctctcgtag gtcagaaaca gcacattgcc cttgttgcgc aggcgccagc cgctatggac
     4441 gtggcgcgcc cacgagccga gcgtcgtatc cggcgagaga tacgcgtcga gccacgcttc
     4501 gacgctcggc atcaacgggc cgaggaccat cgcgcgcgtg aagtggtagc tcgagacgaa
     4561 cacgtccttc ggatcgcgca cgacgcagat ggagcgcgcc gcgggcgagt acggcagcgc
     4621 gtcgagagcc atgtgcgtct tgatgatgcg caaccccgtc ggcgccgagc gctgcggctc
     4681 ctcgttcgcg agcgggatcg cgtagtcggc gcgcatgttc atgtcgggcc acgcgacgag
     4741 atcgtggatg tgctcgaact cggcgcgccc gcgatacgcg atctgcaagg caatttgcat
     4801 cgtccagttc gtgccgctct tgaaatacga gcagaccagc acgtcgtgag ccgtcggcgc
     4861 gtagtcttgc gggaagcgcg tcagcagctt cgtgatcgtg cgcgtcaccc aacgcggcca
     4921 acgcttcgcg aacagcttcg tcacgggcca atagatcgcg agaatcagcg cgccgatcgg
     4981 cgccatgagc cagcgcggcg gcggaggccg tcggcgccag cgtttctgcg tggtagcgct
     5041 catgccgcgc cactataccc cttggattta cgcagtcgcg ctcagtaggc tttggcccag
     5101 acgacgcgct gcgcgctcgg cttgcccgtg aacgggcacg tgcccgcctc gctcttgtcg
     5161 agcgggatgc agcgcaccgt gacggacaaa tcgtccttga tctttttttc gaggtcgacg
     5221 tcgccgctga agtgagtcag cgcgaagccc gcatgaatcg gcgtcggcac gtcgtcggcg
     5281 acgcgcgggc tcgcgaagaa cttgtagaac tcgtcgcggt cgtcaatgac ccgcgtgtgc
     5341 tccttgcgat aggccgcggc cttctcgaac aggccggtct ggattgcttg cagcgtgctc
     5401 gcgatcgtca gtatgaattc cgtgcgcgag atgctctgct tatccttcgg cgcacgatcg
     5461 cggcgagccg cgaacacggc gtccttgtcg atgtcgcgcg gcccgatctc gagccgcagc
     5521 ggcactccgc ggcggaccca gtgccagacc ttctcgccgc cgcgctcgtc gcgctcgtcg
     5581 accagcgcgc gaaccggcgc gtcgttgaag cgctgcgcgc gcagctcgac cgcgagcttg
     5641 cggcagtagt cgagcacgcg cgccttttcg tcgtcggaac gatagatcgg gatgatcacg
     5701 acgtgctgcg gcgcgagctt cggcggcaat acgaagccgt cgtcgtcggc gtgcgtcatg
     5761 atcaggccgc cgatgaggcg cgtcgagacg ccccagctgg tcgcccaggc gtgctcgcgc
     5821 ttgccctgct cgttctggaa ggtgacgtcg aacgccttgg caaagttctg gccgaggaag
     5881 tggctggtcc ccgcctgcag ggccttgccg tcctgcatca tcgcctcgat gcagagggta
     5941 tactccgccc cggggaactt ctgcccgtcg gtcttcgggc cgacgagcac gggcat
//
LOCUS       DATLEE010000393         4854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5656391, whole genome shotgun sequence.
ACCESSION   DATLEE010000393 DATLEE010000000
VERSION     DATLEE010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5656391"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..1750
                     /locus_tag="VM692_08420"
     CDS             347..1750
                     /locus_tag="VM692_08420"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HUQ52234.1"
                     /translation="MLEARAAELATRPHSLPPTPEGGSARLTYDQYRSIRFDPAAAIW
                     RNENRNFVLDLLYPGFVFDVPVNINLVVGGTARRVLFKNEIFSYGSSVPQPVAASGNF
                     AGYSGFRVRAPLNAPDYFDEFLIFQGASYFRGAAKGQLFGLSARGLAVRTARPEGEEF
                     PYFTDFWIERPPEQAQQVVIHALLQSPSVVGAYTFTARPGPETIVDVQVKLFPRFELT
                     AFGIAPLTSMFLFDASNRARFDDYRDAVHDSDGLQIMSGRGERLWRALANPRTLKGSY
                     FADENPKGFGLVQRKRQFGDYGDPDAQFDRHPSLWVEPGGEWGAGHVELIEVPSPRRA
                     NDNIFAYWQPHTPIPAGQTAEFSYRLRFTAEPLDDSLARVVATRVGQSLSEEGQRSFV
                     IDFKGAGDIPDNLVPEVSSSAGKVFAPQGHVVAQTGAYRVAFELDPEREDLIELRAVL
                     YSNGQPWAETWLYQWSR"
     gene            1747..3870
                     /gene="mdoH"
                     /locus_tag="VM692_08425"
     CDS             1747..3870
                     /gene="mdoH"
                     /locus_tag="VM692_08425"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HUQ52235.1"
                     /translation="MTTRQPGSVPPFAGLSMPEQNLRRYVRERRLAWPSLKTILARIV
                     TFGGAVALTIYATYEMVAVVSLGIVSTLQWAMVALFAITFCWISLAATSAVSGLLFGG
                     VRRRATGETRVDQRTALVMPVYNENPAGTFAALLAMAEALLDREATDFEIFVLSDTTR
                     PEIYVKETAAYHVLREKLGDRMRVWYRRRSDNAGRKTGNLHDFVTRWGGRYDFMIVLD
                     ADSILAPDTLITLAREMAADPNLGLLQTVPRLAGGRTLFARLQQFAGRVYGPIVARGI
                     ASWQGDDGNYWGHNAIIRVRAFAAAAGLPILPGKKPFGGAILSHDFVEAALLRRAGWS
                     VRMLPTLGGSWEDSPPSLLDVAARDRRWAQGNIQHLAVILSQGFTWSNRAHMGIGVMS
                     YLASPLWFALIVVGLGTAGHIATVQFEYFTDEFSLFPRWPLFDSERMIRLFLIAMLTL
                     LTPKILGILRSFVDGELLRTVNPLRVILGAVAETVISALYAPIMMMIQVRQVSEILIG
                     QDSGWSTQSRKHSRTPWGTLLRRHWLQMLSGVVVSAVLLYVSPPLFLWMSPALLGLVC
                     ALPLSAASGSTLLAKIARFLGLLTVPEEVNVPRVIALRNEAEARLEELLDGMTIERLL
                     SDEVARQRHFAAVQPRTAATRGHPDVMFITARAKLGDAHTAAEALSWLSPPERLAVLG
                     DRDLFHVLVRLVNPDVPDRPALRSA"
     gene            3956..>4854
                     /locus_tag="VM692_08430"
     CDS             3956..>4854
                     /locus_tag="VM692_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52236.1"
                     /translation="MRAQQNLSFEELDDGAAVRGWTFGGADAAVDDVFAVAGRRSLKL
                     TRTTAQGITRVTQRVPAAPLRGGGSAQLVRLVGFVRTATPQVSGALWLRIDGPHGPLF
                     VDSYGSGREPTEREPIVVAGEGDSGWRRLEIELPLPDDVDEVAFGVSLRGQGTAWYDA
                     LELTTIATDAWPAAAPAAVRYLEAALAVMREHSLRRAQVDWAMVRERALAYARGAGRP
                     ADAYLAVRYAVRELGDRHSYLQSPAVSRVLATSAVSNARTGAAATAPSGQLLGSVAHI
                     AIPGFAGGTPAEQVEFAEELRKI"
BASE COUNT          717 a         1581 c         1654 g          902 t
ORIGIN      
        1 caaagtcaat tacgcggagg gcggctattg tagggccctt gcgtggggcg cacagccagg
       61 gccggcggga atcctggtgc cgattggcta caacgagcgg gccggaggcg gcacggggat
      121 tgctttagtc ctactttggt agaattcgat tccccggcgg attacccgtc gggcggtcaa
      181 acaagtaatg ctgcgccgtc atctattgtc attgtgctgg cttaccgcgt gtgccgccgg
      241 ctttggctgg gcgcaagagc ccaaccgcgc tccggagacg gactcgacaa cggagccggc
      301 tcgcgcaccg gagccggagc gcccgacgga gccgttcgac cgcgcgatgc tcgaggcccg
      361 cgccgcggag ctggcgacac gtccgcattc gctgccgccg acgccggaag gcggcagcgc
      421 gcgtttgact tacgatcaat accgctcgat ccgtttcgat cctgccgcgg cgatctggcg
      481 caacgagaat cgtaatttcg tcctggacct cctttacccc ggcttcgtct tcgacgtgcc
      541 ggtcaacatc aacctcgtcg tcggcggcac ggcccgacgc gtgctgttca aaaacgagat
      601 tttttcttat ggctccagcg tgccgcagcc cgtcgccgcg tcgggcaact tcgcgggtta
      661 ctcgggcttc cgcgtgcgtg cgccgttgaa tgcgcccgac tatttcgacg agttcttgat
      721 ctttcaggga gcgagttatt ttcgcggcgc cgcgaaaggg cagctcttcg gcttgtccgc
      781 gcgcggtctc gccgttcgca cggcaaggcc ggagggagag gagtttccgt atttcaccga
      841 tttttggatc gagaggcccc cggagcaagc acagcaggtc gtcatccacg ctttgctgca
      901 gagcccgtcc gtcgtcggcg cctatacgtt cacggcaagg cctggcccgg agacgatcgt
      961 cgacgtgcag gtcaagctat ttccgcggtt cgagttgacg gcgttcggca tcgcgccgtt
     1021 gacctcgatg tttttgttcg acgcgtcgaa tcgtgcgcgc ttcgacgatt accgcgacgc
     1081 cgtgcacgac tcggacggcc tgcaaataat gtccggccga ggcgagcgat tgtggcgcgc
     1141 cctagccaac ccgcgcacct tgaaagggtc gtacttcgcc gacgagaacc cgaagggctt
     1201 cggcctcgtg cagcgcaagc gccaattcgg cgactacggt gatccggacg cgcaattcga
     1261 tcggcaccct tcgttgtggg tcgagccggg gggcgagtgg ggcgcggggc acgtcgagct
     1321 catcgaggtg ccgtcgccgc gccgcgcgaa cgacaacatc ttcgcgtatt ggcagccgca
     1381 tacgccgatc ccggcaggcc agaccgccga gttctcgtat cgattgcgtt tcactgcgga
     1441 gccgctcgac gattcgctcg cgcgcgtcgt tgcgacgcgc gtcggccagt cgttgagcga
     1501 ggaaggtcag cgctcgttcg tgatcgactt caaaggtgcc ggtgacattc cggacaacct
     1561 cgtgcccgag gtctcgagct ccgcgggcaa ggtgtttgcg ccgcagggcc acgtcgtcgc
     1621 ccaaacgggc gcctatcgcg tcgcgttcga gctcgacccc gagcgcgaag acctcatcga
     1681 gctacgcgcg gtgctctatt cgaacggcca accctgggcc gaaacgtggc tgtatcagtg
     1741 gagtcgatga cgacacgaca gccgggctcc gtgccgccgt tcgccgggct ctcgatgccc
     1801 gagcagaatc tgcggcgcta cgttcgcgag cgcaggctcg cctggccgtc gttgaagacg
     1861 atcctagccc gcatcgtgac gttcggcggc gccgtggcgc tgacgatcta cgcgacgtac
     1921 gagatggtcg ccgtcgtgtc gctcggtatc gtgtcgacgt tgcagtgggc aatggtcgcg
     1981 ttgttcgcga tcacgttctg ctggatctcg ctcgccgcga cgagcgcggt gtccggcctg
     2041 ttgttcggcg gcgtccgtag gcgggcgaca ggcgaaacgc gcgtcgacca acgcacggcg
     2101 ctcgtcatgc cggtctacaa cgagaaccct gccgggacgt tcgccgcgct gctcgcaatg
     2161 gcggaggcgc tgctcgatcg tgaggcgacg gacttcgaga ttttcgtgct gtccgacacg
     2221 acgcggcccg agatctacgt caaggagacc gccgcttatc acgtgctgcg cgagaagctc
     2281 ggcgaccgta tgcgcgtgtg gtatcggcgc cgttccgaca acgccggccg caagaccggc
     2341 aacttgcacg acttcgtgac tcgctggggc gggcgttacg acttcatgat cgtgctcgat
     2401 gccgacagca tcctcgcgcc ggacacgctg atcacgctcg cgcgcgagat ggcggccgac
     2461 ccgaacttgg gcctgctgca gaccgtgccg cgactcgcgg gcggtcggac gttgtttgcg
     2521 cggctgcagc agtttgccgg ccgcgtgtac gggcccatcg ttgcgcgcgg cattgcgagc
     2581 tggcagggcg acgacggcaa ttactgggga cacaatgcga tcatccgggt gcgcgcgttc
     2641 gccgcagccg ctggtttgcc gatcctgccc ggcaagaagc cgttcggcgg cgcgatcttg
     2701 tcgcacgatt tcgtcgaggc cgctttgctg cgccgcgccg gttggtcggt gcgcatgctg
     2761 ccgacgctcg gcggcagctg ggaagacagc ccgccgtcgc tgctcgacgt cgcggcgcgc
     2821 gaccggcgtt gggcgcaagg caacattcag cacctcgccg tgatcttaag ccaagggttc
     2881 acgtggtcga accgcgctca catgggcatc ggggtcatga gctacctggc ttcgccgctc
     2941 tggttcgcgc tgatcgtcgt aggcctaggc acggcggggc acatcgcgac ggtgcaattc
     3001 gagtatttca ccgacgagtt ttcgctgttc ccccggtggc cgttgttcga cagcgagcga
     3061 atgatcaggc tgttcctcat cgcgatgctc acgttattga cgccgaagat cctcggcatc
     3121 ttgcgttcgt tcgtcgatgg cgagctgttg cgcaccgtga atccgctgcg cgtgattctc
     3181 ggcgccgtcg cggaaaccgt gatctctgct ttgtacgcgc cgatcatgat gatgattcaa
     3241 gtccggcagg tgtcggagat cctgatcggc caagattccg gctggtcgac gcagagccgc
     3301 aagcactccc gtacgccgtg gggcacgttg ttgcgccgcc attggttgca gatgctgtcg
     3361 ggcgtcgtcg tctccgccgt cctgctctat gtgtcgccgc cgttgttcct ctggatgtcg
     3421 ccggcgttgc tgggcctcgt ttgcgcattg ccgttatcgg ccgcgagcgg gagcactttg
     3481 ctcgcgaaga tcgcgcgctt cctcggcttg ctgacggtgc ccgaagaagt caacgtgccg
     3541 cgcgtgattg cgttgcgcaa cgaagccgag gcgcggctcg aggagttgct cgacggcatg
     3601 actatcgagc gcttgctcag cgacgaggtc gcgcgacagc ggcacttcgc tgccgtgcag
     3661 ccgcgcacgg ccgcaacgcg cggccatccc gacgtcatgt tcatcacggc gcgcgccaag
     3721 ctcggggacg cgcataccgc ggctgaagcg ctgtcgtggc tgtcgccgcc tgagcgattg
     3781 gccgtgctcg gcgatcgcga tctgtttcac gtgctcgtgc ggctcgtcaa tcccgatgtt
     3841 cccgaccggc ccgcgttgcg ctcggcctga gcgatcgcag cggccggcgc gcgcttccct
     3901 gcgcgctgct cgactgacct ccatcgtcat cgcgtcgctc gctgcgacta cgccgttgcg
     3961 cgcgcagcaa aatttgagct tcgaggagct cgacgacggt gcggcagtgc gaggctggac
     4021 tttcggcgga gcggatgccg cggtcgacga tgtgttcgcc gtcgcaggcc ggcgcagcct
     4081 caagctgacg cgcacaacgg cgcaagggat cacgcgagtg acgcagcgcg tcccggcggc
     4141 gccgctgcgc ggcggcggca gcgcgcaact cgtacgcctc gtaggcttcg tgcggacggc
     4201 aacgccgcag gtgtccggtg cgctgtggtt acgcatcgat gggccgcacg gcccgctgtt
     4261 cgtcgacagc tatgggtcgg gccgcgagcc gacggagcgc gagcccatcg tcgttgcagg
     4321 cgagggcgat tcgggttggc gccggctcga gatcgagctg ccgctcccgg acgacgtcga
     4381 cgaggtcgcc ttcggcgtga gtctgcgcgg ccagggcacg gcctggtatg acgcgctcga
     4441 gctcacgacg atcgccaccg acgcgtggcc cgccgccgcg ccggcggccg tgcgctatct
     4501 cgaggccgcg ttggccgtca tgcgcgagca ttcgctacga cgcgcgcagg tcgattgggc
     4561 catggtgcgc gaacgtgcgc tcgcgtatgc gcgcggcgcc ggcaggccgg ccgacgccta
     4621 cctggccgtg cgctacgccg tacgcgagct cggcgaccgg cacagttatc tgcagagccc
     4681 ggccgtctcg cgcgtgctcg cgacgagcgc ggtctcgaac gcccgcacgg gcgcagccgc
     4741 gacggcgccg agcggccaac tgctgggtag cgtcgcgcac attgcgattc ccggcttcgc
     4801 cggcggcacg cccgcggagc aggtggagtt cgccgaagag ctcaggaaga tcat
//
LOCUS       DATLEE010000394         4037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5656424, whole genome shotgun sequence.
ACCESSION   DATLEE010000394 DATLEE010000000
VERSION     DATLEE010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5656424"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VM692_08435"
     CDS             <1..1054
                     /locus_tag="VM692_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ52237.1"
                     /translation="GNATWNATDVFRVNFGGRYQDATKDGRLPAEVAFLVGPTARNFG
                     PFAPIPANQVGGVPAVGTVEASEFLPEVGVEWDMSADIMGYVKYAEAFKNGGFVMSPP
                     VGGGLPNPFSFAPEFAEGYEVGLKTRLADNRVELNAAAYRTDYTNLQVTVFISAIGRF
                     ITTNAAEAHTQGFEFDGRWAINDAFQLGFAGAFASEAVYDAYDGASCNSLEAKSVPPP
                     CRADRSGVSLPYAPDWTISLNPEYRAMVGRNFDLIVGANVRFSDGYWISDNEDPRNEV
                     DAFERVDLRIGLAPRDGRWEAAIYGRDLTDERLTVGSQPDFQHKSADPTLYDAFGNTR
                     ERGRRYGIQFNYNFGG"
     gene            complement(1036..1290)
                     /locus_tag="VM692_08440"
     CDS             complement(1036..1290)
                     /locus_tag="VM692_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52238.1"
                     /translation="MRKLRRAPSEATASKRVRHFDETWQRDDAEDIKFNADYAIGENV
                     ELVGVRLRSLECTCAATTEAEAPAVDAPASDNEPAQPPKL"
     gene            complement(1358..1930)
                     /locus_tag="VM692_08445"
     CDS             complement(1358..1930)
                     /locus_tag="VM692_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUQ52239.1"
                     /translation="MSAMLPSDCAQAEQVYGFVIGALPASEAAQFVLHLAACTSCRSD
                     VETLRPIMASFVHWPSDVLRPSPSLWERLSARIEGIAGDPAATRPPSRRFEWKEVAPG
                     ISCKLLATDLANDRVSMLVRLAPNTEYPPHTHSGVEELHLLHGELWINERKLYPGDYN
                     RAEPGTSDARVWSETGCTCVLITSPSDQLG"
     gene            complement(1927..2553)
                     /locus_tag="VM692_08450"
     CDS             complement(1927..2553)
                     /locus_tag="VM692_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUQ52240.1"
                     /translation="MNSPRAEAPGDRPSDTLGSVLYANSAEPLVSEADWVDLVHAIAG
                     HSEVALKELYGRTHGFTFTLIFRIVKDPLTAEEVLLDVYHDVWRNAATFDVTRGTVVA
                     WLMNQARSRAIDRLRTENRKKRSQSYEPTLVVETIPSSAQEQRNALEQCLQGLTPDER
                     RAIETAYFQELTYAEVAQRLGEPLGTIKTRIRTGLARLRTLLASKEGL"
     gene            complement(2609..3691)
                     /locus_tag="VM692_08455"
     CDS             complement(2609..3691)
                     /locus_tag="VM692_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HUQ52241.1"
                     /translation="MKSALLGGLAALLAATAVAQPAQTPFKLGTFERQGRTFVGIVLR
                     EAQVIDLAAAHAAITSPPATVAAPTDMKDLIARYDSGLRARIVDIVRTVGNTRPAYVY
                     DVSALKILPPIMYPTTMLNVAVNYREHDIEMARVRQGDTTQAAPTAGAAPTGTVSAPG
                     IWQRAADDTRWNPFMFIKLPGSIVAEGEAVRLPVGRTQIEWECELGVVIGRPAARVTQ
                     ARAMDYVFGYTLENDMSDRGGRGDSRFGSDWLITKNHDTFAPMGPFITPKEFIADIGN
                     TGMKFSLNGELLQEGTTAQMIHNVPEQIVYASNIVTLRPGDVIATGTPPGVGSARNPP
                     ILLKAGDKMTCTYDGVGTLTNPVVAP"
     gene            complement(3799..>4037)
                     /locus_tag="VM692_08460"
     CDS             complement(3799..>4037)
                     /locus_tag="VM692_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005901063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EmrB/QacA family drug resistance transporter"
                     /protein_id="HUQ52242.1"
                     /translation="AALSENITPFRDAFQVPGVPTIWNWSTELGRIALDAQVTRQALT
                     IAYINDFRFMMYLSMLALPLLLLLRPPQRAKAAR"
BASE COUNT          693 a         1292 c         1321 g          731 t
ORIGIN      
        1 cggcaacgcc acgtggaatg cgaccgacgt cttccgcgtg aacttcggcg gccgctatca
       61 ggacgcgacg aaggacgggc gcttgccggc cgaggtcgcg ttcctcgttg ggccgaccgc
      121 gcgcaatttc gggccgttcg cgccgatccc ggcgaaccaa gtcggcggcg tgccggccgt
      181 cggcacggtc gaggccagcg agttcttgcc cgaggtcggc gtcgagtggg acatgtctgc
      241 cgacatcatg ggctacgtca aatacgccga ggccttcaag aacggcggct tcgtgatgtc
      301 gccgcccgtc ggcggcggcc tgccgaaccc gttcagcttc gcgccggagt tcgccgaagg
      361 ttacgaggtc gggctgaaga cgcggcttgc cgataaccgt gtcgagctga acgcggccgc
      421 gtaccggacc gactacacga acctgcaggt gacggtgttc atcagcgcga tcggccgctt
      481 catcacgacg aacgcggccg aggcccacac gcagggtttc gagttcgacg gccgctgggc
      541 catcaacgac gcgttccagc tcggcttcgc gggcgctttc gcctccgaag ccgtctacga
      601 cgcttacgac ggcgcgtcgt gcaactcgct cgaggcgaag agcgtgccgc cgccgtgccg
      661 cgcggatcgt tccggcgtca gcttgccgta cgctccggat tggacgatct cgttgaatcc
      721 cgaataccgc gcgatggtcg gcaggaactt cgatctcatc gtgggcgcca acgttcgctt
      781 cagcgacggc tactggatct cggacaacga ggatccgcgc aacgaggtcg acgccttcga
      841 gcgcgtagac ctacgcatcg gcttggcgcc gcgcgacggg cgctgggagg ccgcgatcta
      901 cggccgcgac ctgacggacg agcgtctcac cgtcggcagt caacccgact tccagcacaa
      961 gtcggcggac ccgacgctgt acgacgcgtt cggcaacacg cgcgagcgcg gccggcgcta
     1021 cggcatccag ttcaactaca acttcggggg ttgagcaggc tcgttatcgc tcgctggagc
     1081 atccaccgcg ggagcttcgg cttccgtcgt ggcggcgcat gtgcactcta ggcttcgtag
     1141 gcgcacaccg acgagctcga cgttctcgcc gatcgcatag tcggcgttga acttgatgtc
     1201 ctcggcgtcg tcgcgctgcc atgtctcgtc gaagtgcctg acacgtttcg acgctgtcgc
     1261 ctcggacgga gccctgcgta gcttgcgcat tgttccaacc ctcgaacgtc gccgagcaag
     1321 gacatgcccg cgatggttcg gccgctacag ccgagagtta gcccagctgg tcgctcggcg
     1381 acgtgataag cacgcaggtg cagccggtct cactccagac acgtgcgtcc gacgtgccag
     1441 gctcggcccg attgtagtcg ccggggtaga gcttgcgctc attgatccaa agctcgccgt
     1501 gcagcagatg aagctcctcg actccggagt gcgtgtgagg cgggtactct gtgttgggtg
     1561 caaggcggac cagcatgctt acgcggtcgt tcgctagatc ggtagcaagc aacttgcagg
     1621 aaatcccagg cgcgacctct ttccactcga accttctgga cggcgggcgc gtggcagctg
     1681 ggtcgcccgc tatgccttcg atccgagccg acagtcgctc ccacaaagaa ggcgacgggc
     1741 gaagtacatc ggacggccag tgaacgaacg atgccatgat cggtcgcagc gtctccacgt
     1801 cgctgcggca actggtgcaa gcggcgagat gcagcacaaa ctgcgcagcc tccgatgcgg
     1861 gcaaagcccc gatcacgaag ccataaacct gctccgcttg ggcgcaatcg gacgggagca
     1921 tcgcgctcat aatccctcct tgcttgccag cagcgttcgc agtctcgcaa gaccggtgcg
     1981 aatgcgtgtc ttgatcgttc caagcggctc gccgaggcgc tgcgctactt cggcataggt
     2041 cagttcttga aagtacgccg tctcgatagc gcgacgctca tcgggcgtta gtccctgtaa
     2101 gcactgttcc aacgcattgc gctgttcttg ggcgcttgaa ggaatcgtct ccacaacgag
     2161 tgtcggctcg tatgattggc tccgcttctt gcggttttcc gtgcgaagac ggtcgatcgc
     2221 tcgcgagcgg gcttggttca tgagccatgc aacgactgtc ccgcgagtaa catcgaacgt
     2281 cgccgcgttc cgccagacgt cgtggtagac gtccagcaaa acctcttccg cggtaagcgg
     2341 gtctttgacg atgcggaaga tcaaagtgaa agtaaacccg tgtgtgcgcc catatagctc
     2401 cttcagagcg acctcactat gacctgcgat ggcgtggacg aggtcgaccc aatccgcctc
     2461 gctaaccagc ggctcggcac tattggcata taaaacggac ccaagggtgt cggacgggcg
     2521 atcaccgggg gcctcggcgc gcggactgtt caccggccgc actgtaaccg tcacaaaccg
     2581 acagcggcaa ggcgggctcg aatccgcctc acggagcgac cacgggattc gtcaacgtgc
     2641 cgacaccgtc gtacgtgcac gtcatcttgt ccccggcctt cagcaggatc ggcggattac
     2701 gcgccgagcc gacgccgggc ggcgtgcccg tcgcgatgac gtcgccgggt cgcaaggtca
     2761 cgatgttgct cgcgtagacg atttgctccg gcacgttgtg gatcatctgc gcggtcgtgc
     2821 cttcctgtag cagctcgccg ttcagcgaga acttcatgcc cgtgttgccg atgtcggcga
     2881 tgaactcctt cggcgtgatg aacgggccca tcggagcgaa cgtgtcgtgg ttcttcgtga
     2941 tgagccaatc ggagccgaag cggctgtcgc cacggccgcc gcgatccgac atgtcgttct
     3001 cgagcgtgta gccgaacacg tagtccatcg cgcgcgcctg ggtgacgcgc gcggccggtc
     3061 ggccgatgac gacgccgagc tcgcactccc actcgatctg cgtgcggccg acgggcaggc
     3121 gcacggcttc gccttcggcg acgatcgagc cgggcagctt gatgaacatg aatgggttcc
     3181 agcgagtgtc atcggccgca cgttgccaga tacccggcgc gctgaccgtg cccgtgggcg
     3241 cggcgccggc cgtcggcgcg gcttgcgtcg tgtcgccttg gcgcacacgc gccatctcga
     3301 tgtcgtgctc gcggtaattc acggcgacgt tcagcatcgt cgtcggatac atgatcggcg
     3361 gcaaaatctt cagcgcgctg acgtcgtata cgtaagcggg gcgcgtgttg ccgacggtgc
     3421 ggacgatgtc gacgatgcgc gcgcgcaagc ccgagtcgta gcgcgcgatg aggtccttca
     3481 tgtcggtcgg cgccgcgacg gtcgctggcg ggctcgtgat cgcggcgtgc gcggctgcga
     3541 gatcgatgac ttgggcttcg cgtagcacga tgccgacgaa cgtgcgtcct tggcgctcga
     3601 acgtgccgag cttgaacggt gtctgagccg gttgcgcgac ggcagtagcc gccaaaaggg
     3661 ctgcgagccc tccgagaaga gcggatttca tgagcgattc cccctggcgt tagctcgcgg
     3721 cccgcgccgc gaagcgattc atcgtactcg cggcgcggtg ccgccgccaa cgcggaaaat
     3781 cgcgcgcgtc agcctaagtc agcgggcggc tttggctcgc tgcggcgggc gcaacaacaa
     3841 caacagcggc agcgccaaca tgctcaagta catcatgaag cggaagtcgt tgatgtaggc
     3901 gatcgtcagc gcctgccgtg tcacttgggc gtcgagcgcg atgcgcccga gctccgtgga
     3961 ccagttccag atcgtcggca cgcccggaac ctggaacgcg tcgcggaacg gcgtgatgtt
     4021 ctcgctcaaa gcggcgt
//
LOCUS       DATLEE010000395         2655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_442022, whole genome shotgun sequence.
ACCESSION   DATLEE010000395 DATLEE010000000
VERSION     DATLEE010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442022"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1299
                     /locus_tag="VM692_08465"
     CDS             25..1299
                     /locus_tag="VM692_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HUQ52243.1"
                     /translation="MLRRMAGMTLEADGCEAPVGSRCVVVDGDGSTTETEVVGFSGGS
                     LLLVATDEIGDVTPGARVIPGGKVFEAYAGPGLLGRVIDGAGQPLDGLGPLRCEDRIR
                     LTPRPMNPLDRKPIREPLDVGVRSINALLTVGRGQRLGLFAGSGVGKSVLLGMMTRFT
                     TADVIVVGLIGERGREVQDFVSQYLTPQTRKRAVIIATPADRPPLVRIHSALLATSIA
                     EYFRDQGKNVLLLMDSLTRVSQAQREIALAIGEPPVTKGYPVSVFAKLPQLVERAGNG
                     GPRGGSITAFYTVLTEGDDPNDPVADAARAVLDGHIVLSRQIAETGLYPAIDIEASIS
                     RLMPLITTPEQQQLIRRFRAMNAAYAQNRDLITIGAYAKGSDPRVDEAIHYWPAIQRF
                     LQQDGNESISFAQSFAALQALFSAERPREVKK"
     gene            1296..1733
                     /gene="fliJ"
                     /locus_tag="VM692_08470"
     CDS             1296..1733
                     /gene="fliJ"
                     /locus_tag="VM692_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HUQ52244.1"
                     /translation="MNKAVRLEPLADYAGKVENEAARRLAACAQALSAKEKEVEGLRG
                     YLAEYRQRAELTDATTDPMRWQNTRAFLAKLSEVVAAREAELVHAVELYRLETERWRD
                     SHVRAKSLDKIVANAAREVLVESAKREQRELDERALWQVLERS"
     gene            1791..2156
                     /locus_tag="VM692_08475"
     CDS             1791..2156
                     /locus_tag="VM692_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HUQ52245.1"
                     /translation="MRDVRWGWVLLGALLANVLVTALITPIALLDGQERLGYVAPPAS
                     FVGAFAVGYWIARKARQRAVLHGLLVGLLAMLIFIPIELSQEITFAHILSFAMKVLGG
                     TVGGIVVARRSASGAAATR"
     gene            2269..>2655
                     /locus_tag="VM692_08480"
     CDS             2269..>2655
                     /locus_tag="VM692_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52246.1"
                     /translation="MKTPIALVASFTLALQLAHAAPSKEPAQLTGTWTLNERSSDDPV
                     RVLNGERGRGGGRNFKVTTGGSIFGVPVGNLPRPAKDEDDDDDEDEEARHDDLQGAEH
                     VFKATYRLRIRREGDVTEIRYGNDPTI"
BASE COUNT          451 a          873 c          898 g          433 t
ORIGIN      
        1 actcgcggct tcgccgcgca tggcgtgttg cgccgcatgg ccggcatgac gctcgaagcc
       61 gacggctgcg aggcgcccgt cggcagccgc tgcgtcgtcg tcgacggcga cggcagcacg
      121 acggagaccg aagtcgtcgg cttctcgggc ggctcgctgc tgctcgtcgc tacggacgag
      181 atcggcgacg tcacaccggg cgcgcgcgtg attcccggcg gtaaggtttt cgaggcctac
      241 gccgggcccg gcttgctcgg ccgcgtcatc gacggcgcgg gccaaccgct cgacgggctc
      301 gggcctttgc gctgcgagga tcgtattcgc ctgacgccgc ggccgatgaa tccgctcgat
      361 cgcaaaccga ttcgcgagcc gctcgacgtc ggcgtgcgct cgatcaacgc gttactgacc
      421 gtcggccgcg gccaacggct cggcttgttc gccggcagcg gcgtcggcaa gagcgtgctg
      481 ctcggcatga tgacgcggtt cacgacggcc gacgtcatcg tcgtcggctt gatcggcgag
      541 cgcggccgcg aggtgcagga tttcgtctcg cagtacttga cgccgcagac gcgcaagcgc
      601 gccgtcatca tcgcgacgcc cgcggaccgg ccgccgctcg tgcgcataca cagcgcgctg
      661 ctcgcgacga gcatcgccga atactttcgc gatcaaggca agaacgtgct gctgctgatg
      721 gactcgctga cgcgcgtctc gcaggcacag cgcgagatcg cgctcgcgat cggcgagccg
      781 cccgtcacga aaggctatcc cgtgtcggtg ttcgccaaat tgccgcagct cgtcgagcgc
      841 gccggcaacg gcgggccgcg cggcggctct attaccgcgt tctacaccgt cctgacggag
      901 ggcgacgatc ccaacgatcc cgtcgccgac gccgcgcgcg cggtgctcga cggccacatc
      961 gtgctgtcgc ggcagatcgc cgagacgggt ctctatcccg ccatcgacat cgaggcgtcg
     1021 atcagtcggc tcatgccgtt gatcacgacg ccggagcagc agcagctgat ccgccgcttc
     1081 cgcgccatga acgccgcata tgcgcagaac cgcgacctca tcacgatcgg cgcgtatgcg
     1141 aagggcagcg acccgcgcgt cgacgaggcg attcactact ggccggcgat tcaacggttt
     1201 cttcagcagg acggcaacga atcgatctcg ttcgcccaga gcttcgcagc attacaggcg
     1261 ctgttctccg cggaacggcc tcgtgaggtc aagaaatgaa caaggcagtc agattggagc
     1321 cgctggcgga ctacgccggc aaggtcgaga acgaggccgc gcggcggctc gccgcgtgcg
     1381 cgcaagcgct gtcggccaag gaaaaagaag tcgaaggctt acgcggctat ctggccgagt
     1441 atcggcagcg cgccgagctc acggatgcga cgacggaccc catgcgctgg cagaacacgc
     1501 gggcgttcct cgcgaaattg agcgaggtcg ttgccgcgcg cgaagcggag ctcgtgcacg
     1561 ccgtcgagct ctatcggctc gagaccgagc gctggcgcga ttcgcacgtg cgcgccaaat
     1621 cgctcgacaa gatcgtggcg aacgcggcgc gcgaagtgct cgtcgaaagc gccaagcgcg
     1681 agcagcgcga gctcgacgag cgagcgctgt ggcaggtcct cgagcgaagc tagcaacccg
     1741 cttccgccgg ggcgtgccct tgcagcgaga cggtgctaac gttcgcttcc atgcgagacg
     1801 tccggtgggg ttgggtgctg ctcggcgcgt tgctggcgaa cgtgctggta acggcgctga
     1861 tcacgccgat tgcgttgctc gacggtcagg agcgcctcgg ctatgtcgcg ccgcccgcgt
     1921 cgttcgtcgg cgcgttcgcg gtcggctatt ggatcgcgcg caaagcgcgg cagcgcgccg
     1981 tcctgcacgg gttgctcgtg ggtttgctcg cgatgttgat cttcataccg atcgagcttt
     2041 cgcaggaaat tacgttcgct cacatcctgt ccttcgcaat gaaggtcctc ggcggcaccg
     2101 ttggcggtat cgtcgttgcg aggcgatcgg cctccggagc cgcggcaaca cgctagccga
     2161 gtcgctgccc gcttcgaacg catgcgcgcc ggacgtgaca gttcgttaca gtgaactcgc
     2221 gtgtcgttcg ctagtcttta gcttcgtagc cacgaacgag gaacgaagat gaagacgccg
     2281 atcgcgctgg tcgcgagctt cacgttggcg ttgcagctcg cgcatgctgc gccgtcgaaa
     2341 gagcccgcgc agctgacggg aacttggacc ctgaacgagc gctcgagcga cgacccggtc
     2401 cgagtgctga acggcgagcg cggccgcggc ggcgggcgca acttcaaagt cacgacaggc
     2461 ggatcgatct tcggtgtccc cgtcggcaat ctgccgcgtc ccgccaaaga cgaggacgac
     2521 gacgacgacg aagacgaaga ggcgcgacac gacgacctgc agggcgccga gcacgtgttc
     2581 aaagctacgt atcgcctgcg cattcgtcgc gagggcgatg tcaccgaaat ccgttacggc
     2641 aacgacccga caatt
//
LOCUS       DATLEE010000396         6777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1931082, whole genome shotgun sequence.
ACCESSION   DATLEE010000396 DATLEE010000000
VERSION     DATLEE010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931082"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VM692_08485"
     CDS             <1..420
                     /locus_tag="VM692_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUQ52247.1"
                     /translation="GRVLVQPDLSTGGNVFVIGDAASVTLADGRRVPGVAPAAKQQGR
                     YVARVIAARVAGNPSPPPFAYRDAGNLATIGRTSAVIEFPWLTLRGFVAWWLWGIAHI
                     YFLVGVPSPVFVSIRWLWEYLTYSRGARLITGREHSS"
     gene            complement(417..1475)
                     /locus_tag="VM692_08490"
     CDS             complement(417..1475)
                     /locus_tag="VM692_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52248.1"
                     /translation="MPQIDFKPLGNTTVIVVACVYAVLLPMALFAGVYGIFLAAMVSL
                     SLFRYSYAILRNVARGWQHFPPPDMDSMNPFSDFAVVFHYAFFGGLAVLFATTPLIDV
                     PLRLAGFAAVALVFPASAAVMGMTNSLGAALNPASVWTIARELGNDYAKLVGVCVLLV
                     VLGGLSGLLWTVSWVLGIVGELFAVWTTMALFLAIGATLRAHRFQFDLLEGADDAEQR
                     DERERHQQWQKEADRAYASVRSGLPAQAYRTIKELIEREKDSLEIYQWTFNAMLAWDE
                     PKHAVMLGERFAKRLWDAGRKVDALELAQRCRKLSPSFVPPAAFTAQLAEYARELGRH
                     RLADDLTQLAAAGRSTAT"
     gene            complement(1478..2239)
                     /locus_tag="VM692_08495"
     CDS             complement(1478..2239)
                     /locus_tag="VM692_08495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HUQ52249.1"
                     /translation="MTEQLGLFESRGDFNAAELASAPLSVRESRRARHLTLRLLPPHT
                     LELVVPSGTRAAEVSAFVHEHRQWIADARRELAQCRPLRSEGWPSRVELRAIGKSWSV
                     SYRHDPQGPVRCRVSGSVLEVATREESRRGADGALRSWLLDEADYHLTPWLLRESQIV
                     GRRPTNVQVRLQRTRWGSCSSGGTVSLNAALLFVEPPLVRYLFIHELCHMIALNHSRK
                     FWSAVARYEPDYEALDRRLTTAWSEIPLWAHSRGR"
     gene            complement(2294..3025)
                     /locus_tag="VM692_08500"
     CDS             complement(2294..3025)
                     /locus_tag="VM692_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52250.1"
                     /translation="MGLTKSLLAAVAVLCASQAAQAEPSAELLDLAARVHYGFYHGDA
                     RTIETAQEALDRLPDSAEVFYYRDFAAVRRMQLGGASRSALIRLDECARRNVAPGLPK
                     PFTADAWVLVAACALLAGDERRRDEALQIARERDDDNPRIVLVEAWALQRAAKGDAAQ
                     SEAVAAKLAAAVEAFDAWTPSIDDPDWGHAEALTALASTALANGQVREARDLIERALL
                     LAPEYREAVELQVALQGARGGNRTL"
     gene            3090..3890
                     /locus_tag="VM692_08505"
     CDS             3090..3890
                     /locus_tag="VM692_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HUQ52251.1"
                     /translation="MHDLIDIGANLTHDSFDADREAVLARAAAAGVARIIVTGTSVTS
                     SVQAGALCETRPQTLFATAGVHPHHAADFDAHTAAALRSLLAARAFVAVGECGLDFFR
                     DYSPRLAQRQAFEAQLELAATVRKPVFLHQRDAHDEFVALLKPVRGRLVGGVAHCFTD
                     GPRELEDYLDLDLYVGVTGWVCDERRGAQLRAAVPLIPLDRLLIETDAPYLLPRDLAP
                     KPQSRRNEPHFLVHVLERIAALRNQSAEALAAATTANAERLFGIARDG"
     gene            complement(3901..4458)
                     /locus_tag="VM692_08510"
     CDS             complement(3901..4458)
                     /locus_tag="VM692_08510"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HUQ52252.1"
                     /translation="MPQYVIAAAIGVGLLLIVVGGWVVWRRNRGPELARALEAVAIER
                     VQNVLLPDGMGGHIQVEHLLLTGHGFLVIDAKPFAGIIFASERMADWTVIGKQGRFTF
                     PNPLGTLYDRVAALKQLVREVPVAGYVLFNGSADFSKGRPRDVLSAAELVERYRRPDP
                     AELERLLVAFAPHWDRVKQAVEPVP"
     gene            complement(4490..5797)
                     /gene="thrC"
                     /locus_tag="VM692_08515"
     CDS             complement(4490..5797)
                     /gene="thrC"
                     /locus_tag="VM692_08515"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HUQ52253.1"
                     /translation="MRYLSTRGGQETGLVDAVMRGLAADGGLFVPDTLPRVDRGSIRG
                     ESLAEIASEVLAPFFAGSGLEAELAAICADAFDFPLPLKPLLADGRELSVLELFHGPT
                     AAFKDVGARFLAALMERALERSPDARPLTILVATSGDTGGAVAAAFHRRTGIRVGVLF
                     PKGQVSPRQQHQLTCWGDNIRAFEIDGVFDDCQALVKAAFSDPNLQRRHRLSAANSIN
                     IGRLLPQVAYHAAASVWHWRLHGTEAGSIVPSGNLGNGMACVWARAIGMPVRELVLAV
                     NANRTIPDYLTTGQWQPRSSVATLASAMDVGNPSNMERLRNLLPNFATLRTNVEAYPV
                     DDASIRAQIAKDHARYGETWCPHTATAFWVYDHLPQARKAGRAWIVSATAHPAKFESI
                     VEPVIGRSVPVPPALEALLERPTAKTLLRPDLAELASELDSWK"
     gene            complement(5804..>6777)
                     /locus_tag="VM692_08520"
     CDS             complement(5804..>6777)
                     /locus_tag="VM692_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HUQ52254.1"
                     /translation="RVRAIGGSKSMALDEQRIDLGKWRDVLAKGAALDADKLVAHIQA
                     DYLPHTVLIDCTASQSVADRYADWLQRGIHVITPNKRAHSGPIAYYEDLKRLSRGSRT
                     HFLYEATVGAGLPIIQTLKDLVATGDEIRSISGIFSGTLAYLFNLFDGTRPFSDIVRE
                     AKAKGYTEPDPRDDLSGTDVARKAVILAREAGLTLELGDIEVESLAPAALANASVDEF
                     LKRLPDFDAPMAERVAAAKRAGKVLRYVATIDVRAGKAQVGLEQFAPTHPFANISLTD
                     NIVQFVTGRYCDNPLIVRGPGAGPAVTAAGIFADLLRLCSMLSGNHS"
BASE COUNT         1170 a         2321 c         2212 g         1074 t
ORIGIN      
        1 gggcgcgtcc tcgttcaacc cgatctatcg acgggcggca acgtattcgt catcggcgac
       61 gccgccagtg tcacgctggc cgatggccgc cgagtgccgg gcgtcgcacc ggcagcgaag
      121 cagcaaggcc gctacgttgc aagagtcatc gctgcacgcg tcgcaggcaa cccgtcaccg
      181 ccaccgtttg cgtaccgcga cgccggcaac ctcgcgacga tcggccgcac gtcggccgtc
      241 atcgagtttc catggctgac gttgcgcggc ttcgttgcct ggtggctatg gggcatcgcg
      301 cacatctatt ttcttgtcgg cgtgccgagc cccgtgttcg tgtcgattcg ctggctatgg
      361 gagtacctga cgtacagccg cggcgcccgg ctgattacgg gccgcgagca ctcgagctaa
      421 gtcgccgtac tgcgaccggc cgccgcgagc tgcgtgagat cgtcggcgag gcggtgacgg
      481 ccgagctcgc gagcatactc ggcaagctgc gccgtgaacg ccgcaggcgg cacgaagctc
      541 ggcgacagct tgcggcagcg ctgcgcgagc tcgagcgcgt cgactttgcg gccggcatcc
      601 cagagccgct tggcgaagcg ctcgccgagc atcacggcgt gtttcggctc gtcccacgcc
      661 aacatcgcgt tgaacgtcca ctgatagatc tcgaggctgt ccttttcgcg ttcgatgagc
      721 tctttgatcg tgcggtaggc ctgcgccggc agcccgctgc gcaccgacgc ataggcgcgg
      781 tccgcctcct tctgccactg ctggtgacgc tcgcgctcgt cgcgctgctc ggcgtcgtcg
      841 gcgccctcga gcagatcgaa ctggaaacga tgggcgcgca gcgtcgcgcc gatggcgaga
      901 aacaacgcca ttgtcgtcca caccgcaaac agctcgccga cgatgccgag cacccacgag
      961 acggtccaga gcagcccgct gagaccgccg agcacgacga gcagcacgca cacgccgacg
     1021 agcttcgcgt aatcgttccc gagctcccga gcaatagtcc acacgcttgc gggattcaac
     1081 gcggcgccca aactgttcgt cattcccatg acggcagccg acgccggaaa cacgagcgcg
     1141 accgctgcaa acccggcgag ccgcagcggc acgtcgatca gcggcgtcgt agcgaacagc
     1201 acggctaagc cgccaaagaa cgcgtaatgg aacacgacgg caaaatcgct gaacggattc
     1261 atcgagtcca tgtccggcgg tgggaagtgt tgccaaccgc gagcgacgtt gcgaagaatc
     1321 gcgtacgagt aacggaacaa cgacaagctg accatcgccg cgagaaagat cccgtagacc
     1381 ccggcgaaca gcgccatcgg taacagcacc gcgtagacgc acgcgacgac gatgaccgtc
     1441 gtgttgccga gcggcttgaa gtcgatctga ggcatagcta ccgcccccgt gagtgggccc
     1501 agagcgggat ttcgctccat gcggtcgtta gccggcgatc gagagcctcg taatccggct
     1561 cgtagcgcgc gacggcactc cagaacttgc gcgagtggtt caacgcgatc atgtgacaga
     1621 gctcgtggat gaacagatag cggacgagcg gcggctccac gaacaacaat gccgcattca
     1681 agctgacggt gccgccgctc gagcaactcc cccaacgtgt gcgttgcagt cgcacttgca
     1741 cgttcgtcgg cctgcggccg acgatctgcg actcgcgcag cagccacggc gtcaaatgat
     1801 agtcggcttc gtcgagcagc caactgcgca aagcgccgtc ggcaccgcgg cgactctcct
     1861 cgcgcgtcgc aacctcgagc accgaaccac taacgcgaca gcgcacgggg ccctgagggt
     1921 cgtgtcgata gctcacgctc cagctcttac cgatggcgcg caattcgacg cggctcggcc
     1981 acccttccga gcgcagcggc cgacactgcg cgagctcgcg gcgggcgtcg gcgatccatt
     2041 ggcgatgctc gtgcacgaac gccgacacct cggcagcgcg agtgccgctc ggcacgacga
     2101 gctcgagggt atgcggcggt agcaatcgta acgtcagatg acgggcgcgg cggctctcgc
     2161 ggacgctgag tggcgcgctt gcgagctcag cggcgttgaa gtcgccgcgc gattcgaaaa
     2221 gtccgagctg ctcggtcaca gaggcgaata caataccagc gggcagcaga acgcaaccgc
     2281 ggtgccgcgt ccgtcacagc gttcgattgc cgccgcgcgc gccctgcagc gcgacttgca
     2341 gctcgacagc ctcgcgatac tccggcgcca gcagcagcgc ccgctcgatg agatcgcgcg
     2401 cctcacgcac ctggccgttg gcgagggcgg tcgacgccag cgccgtcagc gcttcggcgt
     2461 ggccccagtc ggggtcgtcg atcgacggcg tccacgcgtc gaacgcttcg acagcggcgg
     2521 cgagtttcgc tgccacggcc tcgctctgcg cagcatcgcc tttagcggcg cgctgcagcg
     2581 cccacgcctc gacgagcacg atgcgcgggt tgtcgtcgtc acgctcgcgc gcgatttgga
     2641 gagcctcgtc gcggcgccgc tcgtcaccgg ccagcaacgc gcacgcggct acgaggaccc
     2701 aagcgtcggc cgtgaacggc ttcggaaggc ccggcgcgac gttgcggcgc gcgcactcgt
     2761 cgaggcgaat cagcgcgctg cggcttgccc cgccgagctg catgcggcgc accgcggcga
     2821 agtcgcggta gtaaaaaacc tcggcagaat cgggcagccg atcgagcgct tcttgagccg
     2881 tctcgatcgt gcgggcgtca ccgtgataaa agccgtagtg cacgcgggcg gcgagatcga
     2941 gcagctccgc tgacggttcc gcttgcgccg cttgcgacgc gcacaagacc gcgactgctg
     3001 ccagcaacga ttttgtcaaa cccatgggcg aaacagtcta aaggaacgca ttgccgcgct
     3061 cgccgcgcgt cgtcacaatc cggcgccaaa tgcatgacct aatcgacatc ggcgccaact
     3121 tgactcacga cagcttcgac gcggaccgtg aggccgtgct cgcgcgtgca gcggcggccg
     3181 gagtcgcgcg catcatcgtg acgggcacgt cggtcacgag cagcgtccaa gccggggccc
     3241 tctgtgaaac gaggccgcaa acgctgttcg cgacggccgg cgtgcatccg catcacgccg
     3301 cggacttcga tgcgcacacg gcagccgcgt tgcgatcgct gctggccgct cgcgcgttcg
     3361 tcgccgtcgg cgagtgcggg ctcgattttt tccgcgacta ttcgccgcgc cttgcacaac
     3421 ggcaagcatt cgaggcgcag ctcgagctgg ccgcgacggt gcgcaagccg gtgttcctgc
     3481 atcagcgcga cgcgcacgac gagttcgtgg cgctattgaa gccggtacgc ggtcgcctcg
     3541 tcggcggcgt cgcgcattgc ttcacggatg ggccgcgcga gctcgaggac tacctcgatc
     3601 tcgacctcta tgtcggcgtc acgggctggg tctgcgacga gcgccgcggc gcgcagctgc
     3661 gcgcggcggt gccgctgatt ccgctcgacc gattattgat cgagaccgac gcgccgtatc
     3721 tactgccgcg cgatcttgcg ccgaagccgc agagtcggcg caacgagccg cacttcctcg
     3781 tgcacgtgct cgagcgcatc gcggcgctga gaaatcagtc ggccgaagcc ctcgcggcgg
     3841 ccacgacggc gaatgcggag cggttgttcg ggatcgcgcg cgacggctag cgtttcgcga
     3901 tcagggcacg ggctcgacgg cttgcttcac gcgatcccaa tgcggcgcga acgcgacgag
     3961 cagccgctcg agctcggccg ggtcgggccg gcgatagcgc tcgacgagct ccgccgccga
     4021 cagcacgtcg cgcggccggc ccttcgagaa gtccgcactg ccgttgaaca gcacgtaccc
     4081 cgcgacgggc acttcacgga ccagctgctt caacgccgcc acgcggtcat agagcgtgcc
     4141 caaagggttt ggaaacgtga accgcccctg cttgccgatg acggtccaat cggccatgcg
     4201 ttcgctcgcg aagatgatgc cggcgaacgg cttggcgtcg atgacgagga acccgtgacc
     4261 ggtcagcaac agatgctcga cttggatgtg gccgcccatg ccatcgggca ggagcacgtt
     4321 ttgcacgcgt tcgatcgcca cggcttcgag agcgcgcgcg agctcggggc cgcggttgcg
     4381 gcgccatacg acccacccgc ccacgacgat cagcaacagc cccacgccga tggctgccgc
     4441 tattacgtac tgaggcatca cgtccggttg ttctcgtaac tagttgtttt tacttccacg
     4501 agtcgagctc gctcgccagc tccgccaggt ccggccgaag cagcgtcttg gctgtcggcc
     4561 gctcgagcag ggcctcgagc gccggcggga cgggcacgct gcgcccgatc acgggctcga
     4621 cgatgctctc gaacttcgcc ggatgcgccg tagcgctgac gatccacgca cgacccgcct
     4681 tacgggcttg cggcaggtga tcgtagaccc aaaacgccgt cgccgtatgc gggcaccaag
     4741 tctcgccgta gcgagcatgg tctttcgcga tctgcgcgcg gatgctcgcg tcgtcgacgg
     4801 gatacgcttc gacattcgtc cgtagcgtcg cgaaattcgg caacagattc cgcaaccgct
     4861 ccatgttgct ggggttaccg acgtccatcg ccgaagccaa cgtcgcaacg ctcgaccgcg
     4921 gctgccactg ccccgtcgtc aaatagtcgg gaatcgtccg attggcgttc acggccagca
     4981 cgagctcgcg gaccggcatg ccgatcgcac gcgcccagac gcaagccata ccgttgccga
     5041 ggttcccgct cggcacgatc gagccggctt ccgtgccgtg cagccgccaa tgccaaacgc
     5101 tcgctgccgc gtggtacgcg acctgaggca gcaagcgtcc tatgttgatg ctgttggcgg
     5161 cagacaaccg gtggcggcgt tgcagattcg ggtccgagaa agccgccttc acgagcgctt
     5221 ggcagtcgtc gaaaactccg tcgatctcga acgctcggat gttgtcgccc cagcaggtga
     5281 gctggtgctg ctgccgcggc gacacctgcc ccttcgggaa cagcacgccg acgcggatgc
     5341 ccgttcgacg atggaacgcc gcagcgacgg cgccgcccgt atcgcccgag gtcgcgacga
     5401 gaatcgtcag cgggcgcgca tcgggcgagc gctcgagcgc tcgttccatg agcgcggcga
     5461 ggaagcgggc gccgacgtct ttgaacgccg ccgtcgggcc gtgaaatagc tcgagcaccg
     5521 acagctcgcg gccatcggcg agcaacggct tcagcggcag cggaaaatcg aatgcatcgg
     5581 cgcagatcgc tgcgagctcc gcttcgagcc ccgagcccgc gaagaacggc gcgagcactt
     5641 cgctcgcaat ctcggcgagc gactcgccgc ggatgctacc ccgatcgacg cgcggcaggg
     5701 tgtccggcac gaacaagccg ccgtccgccg cgaggccccg catgaccgca tcgacgagac
     5761 ccgtttcctg accgccgcgc gtgctgagat aacgcatcgc cgatcaactg tgattgccgg
     5821 acagcatgct gcagagccgc aggaggtccg cgaaaatgcc ggccgccgtg accgccgggc
     5881 ccgcgccggg cccacgaacg atcagagggt tgtcgcagta gcggccggtc acgaactgca
     5941 cgatgttgtc cgtcaggctg atgttcgcga atggatgcgt cggcgcgaac tgctcgagcc
     6001 cgacttgcgc tttgccggcg cgcacgtcga tcgtcgcgac gtagcgcagc accttgcccg
     6061 cccgtttcgc ggcggcgact cgctccgcca tcggcgcatc gaaatccggc aggcgcttca
     6121 agaactcgtc gaccgacgca ttcgcgagcg ccgcaggcgc caggctttcc acctcgatgt
     6181 cgccgagctc gagcgtcagg ccggcctcgc gcgcgaggat gacggccttg cgcgcgacgt
     6241 cggtccccga cagatcgtcg cgcgggtcgg gctccgtata acccttggcc ttcgcctcgc
     6301 gcacgatgtc cgaaaacggc cgcgtgccgt cgaacaagtt gaacaggtac gccagcgtgc
     6361 ccgagaagat gccgctgatg ctacggatct cgtcgcccgt cgcgacgagg tccttcaacg
     6421 tctgaatgat gggtaagcct gcgcctaccg tggcctcgta gaggaaatgc gtgcgcgagc
     6481 cgcggctcaa gcgcttcaga tcctcgtaat aggcaatcgg tccgctgtgc gcacgcttgt
     6541 tcggcgtgat gacatggata ccgcgttgca gccagtcggc ataccgatcc gcgacgctct
     6601 ggctcgccgt gcaatcgatc aggaccgtgt gcggcagata gtcggcctga atgtgcgcga
     6661 cgagcttgtc ggcgtccaac gccgcgcctt tcgcgaggac gtcgcgccac ttgccgagat
     6721 cgatgcgctg ctcgtcgagc gccatgctct tgctgccgcc gatcgcgcgc acgcgca
//
LOCUS       DATLEE010000397         3959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8418328, whole genome shotgun sequence.
ACCESSION   DATLEE010000397 DATLEE010000000
VERSION     DATLEE010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8418328"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..388
                     /locus_tag="VM692_08525"
     CDS             131..388
                     /locus_tag="VM692_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52255.1"
                     /translation="MQTCFRTLLYENEGMRHIFLEEAAEYCPQKVQDGETDAEVEKLV
                     RMGGNASLAITLINQRARNRTHRRSPPSKRCEQNRRIWAND"
     gene            complement(566..642)
                     /locus_tag="VM692_08530"
     tRNA            complement(566..642)
                     /locus_tag="VM692_08530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(606..608),aa:Arg,seq:acg)
     gene            complement(733..1815)
                     /gene="alr"
                     /locus_tag="VM692_08535"
     CDS             complement(733..1815)
                     /gene="alr"
                     /locus_tag="VM692_08535"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HUQ52256.1"
                     /translation="MSTDSLHPATLRVDLDALAYNFRLLSERALPAECSAVVKADAYG
                     LGVERVVNRLLREGCRRFFVATVDEARDVRSLAPDVEIGVFEGALSTTVAALVALDAR
                     PVLNSLEQIERWRGHGRALLHLDTGMNRLGLSSGDVAALAVRKDVLADLPLDFVMTHL
                     ACADEPAHPMNAEQLLRFESMRRALPSAPTSIGNSAGALLGAEYRGDLVRPGIALYGG
                     NPFSDRPNPMRGVVTLTAPILQLREIGEPQTVGYGATYLAQAPARIAVVGIGYADGYP
                     RNLGNVGVAAVHGTRVPVVGRVSMDLIAVDVGGLPRDTVRVGDAVELIGPTIGVDEVA
                     DAAGTISYEILTGLGNRPARLYVESA"
     gene            complement(1805..3016)
                     /locus_tag="VM692_08540"
     CDS             complement(1805..3016)
                     /locus_tag="VM692_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HUQ52257.1"
                     /translation="MKPRRDNLVERPWGGSRLCEFKSLTAAPCGTGRRFGESFELSAD
                     DGDEEARQHPSVLPLEDGSTITLPALLALHAETVLGADFVQRHGRRLPLLPKLLDVAE
                     LLSVQAHPPGNTEVYVVVEADAGATIRLGFAADVDADVWAEQLASGRRDQQRLVELCA
                     NSADELQTVLKPWLARRAAKPAELEAALRPRGTTGRWPEIESRLAALHECYWTALDSL
                     NAIPVKAGDVIYNANPARIALTTGKPVSAEVHALGNTEGRGVLALEIRRPGTTFRAWD
                     NVRFPLRAIDIAAALEALNLKATKPADFVVAPQPVRPGVRRSVDSEYFRLEHLDPTPR
                     LAVDVRESPPHCLHALSGTVNVTRGGGELLGTLERGESALVPARVGAYRVAADGERAS
                     LVKVDLPPYVD"
     gene            3225..>3959
                     /locus_tag="VM692_08545"
     CDS             3225..>3959
                     /locus_tag="VM692_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52258.1"
                     /translation="MANPRELAEQAIALLQNALSESEARASDLDEQLKRKRAPKTRLE
                     EQLDVLTHRLESVEAERQRWEQQAGHLEEIAEAERVKVAQLKKKLEIAEAGPEKLTKK
                     EINFWKQKSEEIDAQIKDYQERLTNVRRELNERDALIEKLRVNGGAADAPPSHPQPES
                     QPEADDELRRQLEQRDAWLAELRLELHELRGQPTPPLETQAEVETLRSKITGLERALT
                     EAHNMRAAAQAELIRTQHEIGERERVL"
BASE COUNT          743 a         1334 c         1298 g          584 t
ORIGIN      
        1 aagcacttcg ggttcgtaag gctgtcggcg ttccatgtca tgcccggtct gcagatgcag
       61 cagcataccc cggaccgact aacgttccgc gcaacgttgt gcgctctcta aggcggattg
      121 gcgccgcatc gtgcagacgt gctttcgcac gctgctctac gagaacgaag gcatgcggca
      181 catcttcctc gaggaagccg ccgagtactg cccacagaaa gtgcaggacg gcgagaccga
      241 cgccgaggtc gagaagctcg tgcgcatggg cggcaacgcg tcgctcgcga tcaccctcat
      301 caatcagcgc gcgcgcaacc gcacacaccg acgttcaccg ccgtcgaaac gctgcgaaca
      361 gaatcgcaga atctgggcga acgattaagc gccgtcgagc gctgagctcg agcaacacag
      421 ccggcacgtc gtcgacgcgt tcgatcgagc gaacgaggcc tacgccatgc ctgttcgacc
      481 acttcgccaa gccagtcgag atttcccagg tctaccctga tttcccaggc tacctagagc
      541 tggctagtat ctaagttatt gaatttggcg cgcccggaga gattcgaact cccgacctcc
      601 ggcttcgtag gccgatgctc tatccagctg agctacgggc gcgtgatgaa tggtctggca
      661 gagctcgtct aagtcggtca aagcgctccg cgggcgccat tctacccgaa ccgggcgccc
      721 ctgcttcaag cctcaggcgc tttcgacgta caggcgcgcg ggccggttgc ccagtcccgt
      781 cagaatctcg tagctgatgg tgccggccgc gtccgcgacc tcgtcgacgc cgatcgtcgg
      841 gccgatcagc tcgacggcgt cgccgacgcg aaccgtatcg cgcggcaggc cgccgacgtc
      901 gacggcgatc aggtccatcg acaccctgcc gacgacgggc acgcgcgtgc cgtgcacggc
      961 cgcgacgccg acgttgccga ggttgcgcgg gtaaccgtcc gcgtagccga tgccgacgac
     1021 ggcgatgcgc gccggcgcct gcgcgaggta ggtcgcgccg tagcctaccg tttgcggctc
     1081 gccgatttcc cgcagctgga ggatcggcgc ggtcagcgtc acgacgccgc gcatcgggtt
     1141 cggccggtcg ctgaacgggt tgccgccgta gagcgcaatg cccggccgca cgaggtcgcc
     1201 gcgatactcg gccccaagca acgcgccggc cgagttgccg atcgaagtcg gcgccgaggg
     1261 caacgctcgg cgcatgcttt cgaagcgcag cagctgctcc gcgttcatgg ggtgcgcggg
     1321 ctcgtcggcg caggcgagat gcgtcatcac gaagtcgagc ggcaagtcgg ccagcacatc
     1381 tttgcgcacg gcaagcgccg cgacgtcgcc cgagctcaag ccgagccgat tcatgccggt
     1441 gtcgagatgc agcagtgcgc ggccatgacc gcgccagcgc tcgatctgct cgaggctgtt
     1501 cagcacgggc cgcgcatcga gagcaacgag cgcagccacg gttgtcgata gcgcgccctc
     1561 gaacacgcct atctcgacgt ccggagcgag cgagcgaacg tcgcgcgcct catcgacggt
     1621 cgcgacgaaa aagcgccgac acccctcgcg cagcagccga ttcacgacgc gctccacgcc
     1681 gagaccgtac gcatcggcct tgacgacggc ggaacactcc gccggcaacg cgcgctcgct
     1741 gagtaaccgg aagttgtacg ccagcgcgtc gagatcgact cgcaacgtcg caggatgcag
     1801 cgagtcagtc gacatacggc ggcaggtcga ctttgacgag cgacgcgcgc tcgccatcag
     1861 ccgcaacgcg ataggctccg acacgagcgg gaaccagcgc cgactcgccg cgctcgagcg
     1921 tgcccagcaa ctcaccgccg ccgcgtgtga cgttgacggt gcccgacagt gcgtgcaagc
     1981 aatgcggcgg cgactcgcgc acatcgactg cgaggcgtgg agtcgggtcg agatgctcga
     2041 gccggaagta ttcgctgtcg accgaccgtc gcacgccggg gcgcacgggt tgcggcgcga
     2101 cgacgaagtc agcgggcttg gtcgccttca gattcaaagc ctcgagcgcc gcggcgatgt
     2161 cgatggcgcg cagcggaaaa cgcacgttgt cccacgcgcg gaacgtcgtg ccggggcgcc
     2221 ggatctcgag cgcgagcacg ccgcggcctt cggtgttacc gagggcgtgg acctcggccg
     2281 aaacgggctt accggtggtg agcgcgatgc gcgccgggtt cgcgttgtag atgacgtcgc
     2341 cggctttgac gggaatcgcg ttcaacgaat cgagcgcggt ccaatagcat tcgtgcaacg
     2401 ccgcgagccg cgactcgatc tcgggccagc ggcctgtcgt gcctcgcggc cgcagtgcgg
     2461 cttcgagctc cgcgggcttg gccgcgcgcc gcgcgagcca cggcttgagc accgtttgca
     2521 gctcgtcggc cgagtttgca cacagctcga ccaagcgctg ctgatcgcga cggccgctgg
     2581 caagctgttc ggcccagacg tcggcatcga cgtcggctgc gaaacccaag cgaatcgttg
     2641 caccggcgtc ggcttcgacg acgacgtaga cctcggtgtt gcccggcggg tgcgcctgta
     2701 ccgacaacag ctcggcgacg tcgagcagct tcggcagcag cggcaagcgc cggccatgac
     2761 gctgcacgaa gtccgcgccg agcacggtct cggcatgcag tgccaacaag gccggcagcg
     2821 tgatcgtgga gccgtcttcg agcggcaaca cgctcgggtg ctgccgcgcc tcctcgtcgc
     2881 catcgtcggc cgagagctcg aacgactcgc cgaaccggcg tcccgtccca caaggcgctg
     2941 cggtcaggct cttgaactcg cacaacctcg agccgcccca cgggcgctcg accagattgt
     3001 cgcgtcgcgg cttcaacagg cctgttgcca gccgctcagc gaccgcgctg tcgccttcga
     3061 gcaaacggtc ggcaatggtg ctaacaggcg gcacagctcg agtcccacgc gaggatggcg
     3121 cagtatgcca cggccgttta tgtcaaaacg tgactgagta ccgcgctcgt tttctcctcg
     3181 ctgtcgtaac ctgccgggca attctgttca ctgtcggtcg agcaatggct aacccgcggg
     3241 aattagccga acaggctatc gcgctactac aaaacgcgct cagcgaatcg gaagcgcgtg
     3301 cgtccgacct cgacgagcag ttgaagcgca agcgtgcgcc gaaaactcgc ctcgaagaac
     3361 agctcgacgt cctgactcat cggctcgaaa gcgtcgaagc ggaacgccag cgctgggagc
     3421 agcaggccgg ccatctcgag gagatcgcgg aggccgagcg cgtcaaggtg gcgcagctca
     3481 agaagaagct cgaaattgcg gaagcgggcc ccgagaagct cacgaagaaa gagatcaact
     3541 tctggaagca gaagtccgag gaaatcgacg cgcagatcaa ggattatcag gaacgtctga
     3601 cgaacgtgcg ccgcgagctc aacgagcgcg acgcgctgat cgagaagctg cgcgtgaacg
     3661 gcggcgccgc ggacgcgccg ccttcgcacc cgcaacccga atcgcagccc gaagccgacg
     3721 acgagctacg ccgccagctc gagcagcgcg acgcttggct cgccgagctg cgcctcgagc
     3781 tgcacgagct ccgcggacaa ccgacgccgc cgctcgagac acaagccgaa gtcgagacgc
     3841 tgcgcagcaa gatcacgggc ctcgagcgcg cgctgaccga agcacataac atgcgcgcgg
     3901 ccgcacaagc ggagctcata cgcacgcagc acgagatcgg tgagcgcgag cgtgtgctg
//
LOCUS       DATLEE010000398         4222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3216471, whole genome shotgun sequence.
ACCESSION   DATLEE010000398 DATLEE010000000
VERSION     DATLEE010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3216471"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VM692_08550"
     CDS             complement(<1..1081)
                     /locus_tag="VM692_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52259.1"
                     /translation="MYHAHFGLEKGLFGDGIAADTAVFRSPKHDQIIAHFKLALGGPS
                     ACLVLRGPAGVGKTTLTAATLRASSTRLALAWLNGTPTNATELLELTLVELGINTIGT
                     TRIERLQLWRQFQAEMRATDSRLFIVVERTEDLSVEVLHALDQLTAPDAMGNPGANVV
                     LLGHATIDEHLAAPLLESLRQRIRVRTDLTPFTEAELQDYVRHQVACAGGRFEQIFAP
                     GAVAALYRHSDGVTRLANTLCETALDLAASQLEKQLTPDLVTRTAVSVLGLAAAAPPT
                     RVAAPAGRETVPVVAPLSAPLVSMASVALPTVSLTVMPATPTVLAVPAAAPTTVIATP
                     IPTPTPAPVAAATSRPAPALAAAAPA"
     gene            1245..1793
                     /gene="def"
                     /locus_tag="VM692_08555"
     CDS             1245..1793
                     /gene="def"
                     /locus_tag="VM692_08555"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006910009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HUQ52260.1"
                     /translation="MAIRPILRLGNPLLREVTAPVTEFSTPALSELVRDMLDTMRAND
                     GAGLAAIQIGVLQRVVVFELAHNPRYPDAEPVPLTVLVNPEIELLGDERELGWEGCLS
                     VPGLRGLVPRHRRLRYRGSDELGQPIDRTVSDFHARVVQHECDHLDGILYPQRIEDMT
                     AFGFQDELVASGELPLAAAAVD"
     gene            1904..>4222
                     /locus_tag="VM692_08560"
     CDS             1904..>4222
                     /locus_tag="VM692_08560"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ52261.1"
                     /translation="MSIRSLELAIAVVLGTAAVAQSSEAPVSAPQAFLESTCIKCHSK
                     EAESAIALFSGFFLDRLDVNDVESHPAEWEKVVRKLRTGMMPPASEPRPDEGQRQAFI
                     SYLEGRLDALAARSPNPGRPALHRLNRTEYANAIHDLLALDVDAKELLPGDDASFGFD
                     NVAGSLGISPVLIERYANAAARIARLAVGDVNMTPEQKTHYVPNFLMQSEHIAGLPFG
                     TRGGLRVEHFFPLDAEYIINVDLMSAVNGIHIGNGVPNEQLELTLDGRRVALFDISKP
                     PPKPPAPAADAEKPKPEGAAEAAQPPPEAPQAAESEEKEGEKWEVRLPVPAGPHVLAT
                     AFIKRNHGLIEDVVQQPSNTLLDPLFNGTPEVTLIAHVGSITVDGPYAASGAGSTPSR
                     ARIFTCQPKQVSEDAGMDARGRATQRAVAELACAREILSGVAYRAYRQPPSTEHMSVL
                     MDFYAQGYEKGGFDKGIETGLQRILSSPQFVFRFERDPANAGDGAYAIGDGELASRLA
                     FFLWSSIPDDELLELASRDRLNRPAVLEQQVRRMLADPKAAALTQNFAGQWLYLRNLA
                     IKDASTLEFPDFDDNLRNSFRRETELLFEHVMRNDLSVLELLTADYTFVDERLAKHYG
                     IPGVYGSDFRKVPVTDPRRQGLLGHGSILLVTSNPNRTSPVNRGVWVLKNILGAAVPA
                     PPPVNIPPLEEAAGDVDFDSLTVRELMELHRGKPFCEGCHKIMDPIGLAMENFDVIGR
                     WRTVDNGNPIDTAGHLVDGTEIDGPVALRAALL"
BASE COUNT          705 a         1408 c         1427 g          682 t
ORIGIN      
        1 ccgcgggagc agcagcggcc aaagccggcg ctggccgcga ggttgcggcc gcgaccgggg
       61 ccggggtcgg ggtcggaatc ggcgttgcga tgacagtcgt cggagccgca gccggcaccg
      121 cgagcaccgt cggagtcgcc ggcatgacgg taaggctcac ggtcggcagc gcgacggacg
      181 ccatcgacac gagcggagcc gacaacggcg cgacgacggg caccgtctcg cgaccggccg
      241 gtgcggcgac tcgcgtcggc ggtgcagccg cggcgagccc caacacggac acggctgtcc
      301 tcgtgacgag gtcgggcgtc agctgcttct cgagctggct cgcagccagg tcgagcgccg
      361 tctcgcacag cgtgttggcg agccgcgtga cgccgtccga gtggcgatac aacgccgcga
      421 ctgcgccggg cgcgaagatt tgctcgaagc ggccgccggc gcacgcgact tggtgtcgca
      481 cgtagtcctg cagctcggct tcggtgaacg gcgtcaagtc cgtgcgcaca cgaatgcgtt
      541 gcctcagcga ctcgagcaac ggcgctgcga gatgctcgtc gatcgtcgca tgaccgagca
      601 gcacgacgtt ggcgccgggg ttccccatgg cgtccggggc cgtcaattgg tcgagcgcgt
      661 gcagcacttc gacggagaga tcctcggtgc gctcgacgac gatgaacaac cgcgagtccg
      721 tcgcgcgcat ctcggcttga aattgccgcc agagctgcag gcgctcgatg cgcgtcgtgc
      781 caatcgtgtt gatgccgagc tcgacgagcg tgagctcgag cagctcggtg gcgttggtcg
      841 gcgtgccgtt gagccacgcg agagcgaggc gcgtgctgct cgcacgcagc gtcgctgcgg
      901 tcagagtcgt cttgccgacg ccggccggac cgcgcagcac gaggcaggcg ctcgggccgc
      961 caagcgccag cttgaaatgc gcgatgatct ggtcgtgctt cgggctgcgg aagacggcgg
     1021 tgtccgcggc gatgccgtcg ccgaataagc ctttctcgag tccgaaatga gcgtggtaca
     1081 tggccctcgg gctgccctcg cgaagtgtcg caggctcggg agtgtgccga ttccgggcac
     1141 cccgttcgtg aaaccccgtc acaacttatg caaaacggct tgggctgccg gatccgagcc
     1201 cgctccatat aattgcaggt cagagagttt ggctcggtgc aaagatggcg attcggccga
     1261 ttctgcgttt gggtaatccg ctattgcgcg aggtcaccgc ccctgtgacg gagttctcga
     1321 caccggcctt gagcgagctc gtgcgcgaca tgctcgacac gatgcgcgcc aacgacggcg
     1381 ccggactcgc cgcaatacag atcggtgtgc tgcaacgagt cgtcgtcttc gagctcgcgc
     1441 acaacccgcg ctatcccgat gccgagcccg tgccgctgac cgtgctcgtg aaccccgaga
     1501 tcgagctctt gggagacgag cgcgagctcg gttgggaggg ctgcctcagc gtgccgggct
     1561 tgcgcgggct cgtgcctcgc catcgacgcc tgcgctatcg cggcagcgac gagttgggcc
     1621 agccgatcga ccgcacggtc agcgactttc acgcacgcgt cgtgcagcac gagtgcgatc
     1681 atctcgacgg gattttgtat ccgcagcgca tcgaggacat gacggcgttc ggttttcagg
     1741 acgagctcgt agcgagcggc gagctgccgc tcgccgccgc ggcggtcgat tgagcagaag
     1801 tgcgtgcttg ccgtggtttc gcgggcctgt tacaacgaat cggttcattt gtgactgctg
     1861 agtcagtccg tgcgtcgggg agcgtatgag cgcaactgcg tcgttgagta tcaggtccct
     1921 cgagctggct atcgccgtcg tgctcggcac ggcggccgtc gcgcagtcga gcgaggctcc
     1981 cgtgtcggcg ccgcaagcgt tcctcgaatc gacctgcatc aaatgccaca gtaaggaagc
     2041 ggagagcgcc atcgcgctgt tctcgggctt cttcctcgac aggctcgacg tcaacgacgt
     2101 cgagtcccat ccggctgaat gggaaaaagt cgttcgcaag ctgcgcacgg gcatgatgcc
     2161 gcccgcgagc gagccgcgcc ccgatgaagg acagcggcaa gcgttcatct cctatctcga
     2221 aggtcgcctc gacgcgctcg cggctcgtag cccgaacccg ggccggccgg cgttgcaccg
     2281 cctgaaccgc acggagtacg cgaacgcgat ccacgatttg ctcgcgctcg acgtggacgc
     2341 caaggagctt ttgcccggcg acgacgcgag cttcggcttc gacaacgtcg cgggctcgct
     2401 cggcatctcg cccgtgttga tcgagcgcta cgcgaacgcg gccgcgcgca tcgcgcggct
     2461 cgccgtcggc gatgtcaaca tgacgcccga gcagaaaacg cactacgtgc cgaacttcct
     2521 gatgcagtcc gagcacatcg cggggctgcc gttcggcacg cgcggcggct tgcgcgtcga
     2581 gcacttcttc ccgctcgacg ccgaatacat catcaatgtc gacttgatga gcgccgtcaa
     2641 cggtatccac atcggcaacg gcgtcccgaa cgagcaactc gagctcacgc tcgacggccg
     2701 gcgcgtcgcg ttgttcgaca tctcgaagcc gccgccgaaa ccgcccgccc ccgctgccga
     2761 tgccgagaag ccgaagcccg aaggcgccgc agaagccgcg cagccgccgc cggaagcgcc
     2821 gcaggccgcc gagagcgagg agaaagaagg cgagaagtgg gaggtgcgac ttcccgtgcc
     2881 cgccggcccg cacgtgctcg cgactgcttt catcaaaagg aaccacgggc tcatcgagga
     2941 cgtcgtccag caaccgagca acacgttgct cgatccgctg ttcaacggca cgccggaggt
     3001 caccttgatc gcgcacgtcg gcagcatcac ggtcgacggg ccgtacgcgg cgtcgggcgc
     3061 cggctcgacg ccgagccgcg cgcggatatt cacgtgccag ccgaagcagg tcagcgaaga
     3121 cgcgggcatg gatgcccgcg gtagagcgac gcagcgagca gttgccgagc tcgcgtgcgc
     3181 gcgcgaaatt ctgtcgggcg tcgcgtatcg ggcgtaccgc cagccgccgt ccacggagca
     3241 catgtccgtg ctcatggact tctacgcgca aggctacgag aagggcggct tcgacaaggg
     3301 tatcgagacc ggtttgcagc gcatcctctc gagcccgcag ttcgtgttcc gcttcgagcg
     3361 cgaccccgcg aacgccggcg acggcgcgta tgccatcggc gacggcgagc tcgcgtcgcg
     3421 gctcgcgttc ttcctctgga gcagcatccc cgacgacgag ctgctcgagc tcgcgagccg
     3481 cgatcggttg aaccggcccg ccgtgctcga gcagcaggtg cgtcgcatgc tcgcggatcc
     3541 gaaggccgcg gcgctgacgc agaacttcgc cggccagtgg ctctacttgc gtaatctcgc
     3601 catcaaggac gcctcgacgc tcgagtttcc ggatttcgac gacaacctgc ggaattcctt
     3661 ccggcgcgag acggagctct tgttcgagca cgtcatgcgc aacgatttga gcgtgctcga
     3721 gctattgacg gccgactaca cgttcgtcga cgagcggctc gcgaagcact acgggatccc
     3781 tggcgtgtat ggcagcgatt tccgcaaggt gcccgtcacc gatccgcgcc ggcaagggtt
     3841 actcggccac ggcagcattt tgctcgtgac gtcgaacccg aaccgcacgt cgcccgtcaa
     3901 tcgcggcgtc tgggtgctca agaacattct cggcgccgcc gtgccggcgc cgccgcccgt
     3961 gaacatcccg ccgctcgagg aggccgcggg cgacgtcgac ttcgactcgc tgaccgtccg
     4021 cgagctcatg gagctgcacc gcggcaagcc gttctgcgag ggctgccaca agatcatgga
     4081 tccgatcggc ctcgcgatgg agaacttcga cgtgatcggc cgctggcgca ccgtcgacaa
     4141 cggcaatccg atcgacacgg ccgggcatct cgtcgacggg acggagatcg acggccccgt
     4201 cgcgctgcgc gcggccctgc tg
//
LOCUS       DATLEE010000399         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8421741, whole genome shotgun sequence.
ACCESSION   DATLEE010000399 DATLEE010000000
VERSION     DATLEE010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8421741"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VM692_08565"
     CDS             <1..429
                     /locus_tag="VM692_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52262.1"
                     /translation="AARMDRPVMLYVFSDGSLSSNGVIDNSVAGRGKGEWTSDNQDTA
                     APFFLVYNPPTRGGRAQLLTSAGLTAAQHQQLGAFSADGNVVRSGTPGANNVNLLVHM
                     VLLNYMALHGEQGQFTTLFPNHGLGDAALLDRFIAFSRIA"
     gene            726..1991
                     /locus_tag="VM692_08570"
     CDS             726..1991
                     /locus_tag="VM692_08570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ52263.1"
                     /translation="MDFPSVGGLPWLFAEPNAALGEWRGRLHFSLQRQEREHAQLAAA
                     LTSKTLSAATRARLETLERGTRDHAVRLRALLAPLELEQHAASYETYLALRTRLPADQ
                     GLTTYYANIHRDWCWGDAENEASFDVLAALLGGASAGRVLVLGTGAGRLAYDLHMRTA
                     ASLTAALDFNPLLLLVAERVTRGETLELYEFPLAPRSDAALLRTLAAPAPARAGLVHV
                     LADAHRPPFRRGAFDTVVTPWLIDILPERFDVLCARVNALLGDGGRWLNFGSLNFHDS
                     DPALRYGLDECRSALAEHGFGELVIEEREIPYLSSPASRHARLERVVSWSARKQRDVK
                     KVPRYHALPEWLVRGTDPVPLGDSFRSQAAATRIHAFLMSLIDGRRSIKDMAKLVVEQ
                     RLMSVEEAEPAIRSFLIKMHDDARRGSTY"
BASE COUNT          313 a          716 c          675 g          336 t
ORIGIN      
        1 gccgcgcgca tggataggcc cgtgatgctc tacgtcttca gcgacggctc gctgtcgagc
       61 aacggcgtga tcgacaactc ggtcgccggc cgcggcaaag gcgaatggac gagcgacaac
      121 caagatacgg cggcgccgtt cttcctcgtc tacaacccgc cgacgcgagg cggccgtgcg
      181 cagctgctga cgagcgcggg gctcacggcc gcgcagcacc agcaactcgg cgccttctcg
      241 gcggacggca acgtcgtgcg ctcgggtacg cccggcgcga acaacgtcaa cttgctcgtc
      301 cacatggtgc tgttgaacta catggcgctg cacggcgagc aagggcagtt cacgacgctg
      361 ttcccgaacc acggtctcgg cgacgctgct ctgctcgacc ggttcatcgc gttctctcgg
      421 atcgcctgat cggtcgagcg gcgcacggca acgacattta gtagcggttt ctccacgcgt
      481 tcacttacaa cggttactcg gccacggatg gctgggtaac cgttgttctt ttttcccctg
      541 cggttttcac ataacgtgat cgatgcacgg gcgccgccgc ggaatcgccg ccacgatcac
      601 gcccgcaacg acggaagacg ctcgcgatga cctccttcga cgatctgcga gagctactcg
      661 cctgcccacg gtgcgacgcc ccgctcgcgg tcgccggtga aatttcgcgc tgcgcgggct
      721 gcggagtgga cttcccgtcc gtcggcggcc tgccctggtt gttcgccgaa ccgaacgcgg
      781 cgctcggcga atggcgcggc cggctgcatt tctcgttgca aagacaagag cgcgagcacg
      841 cgcagctcgc agcggcgctg acgagcaaga cgctgtccgc ggcgacgcgt gctcggctcg
      901 agacgctcga gcgcgggacg cgggaccacg ccgtacgact ccgtgcattg ctcgcgccgc
      961 tcgagctcga gcagcacgcg gcgagctacg agacctacct cgcgctacga acgcggctgc
     1021 ccgcggatca agggctcacg acttactacg cgaacatcca ccgcgattgg tgttggggcg
     1081 acgccgagaa cgaagcctcg ttcgacgtgc tggccgcgct gctcggcggc gcgagcgcgg
     1141 gtcgagtgct cgtgctcggc acgggcgcag gccgcctcgc ctacgatctg cacatgcgaa
     1201 cggcggcgtc gttgacggcc gcgctcgatt tcaatccact gctactgctc gtcgccgagc
     1261 gcgttacgcg cggcgagacg ctcgagctct atgagtttcc gctcgcgccg cgcagcgacg
     1321 ccgcgctgct gcgaacgctt gcggcaccgg cgccggcgcg cgcggggctc gtccacgtgc
     1381 tggccgacgc ccatcggccg ccgttccggc gcggcgcttt cgacaccgtc gtgacgccgt
     1441 ggctcatcga catcctgccc gagcgcttcg acgtgctgtg cgcgcgagtg aacgcgctgc
     1501 tcggcgacgg cggccggtgg ctgaatttcg gctcgctgaa ttttcacgac tcggatcccg
     1561 cactgcgcta cggcctagac gagtgccgct cggcgctcgc ggagcacggc ttcggcgagc
     1621 tggtcatcga ggagcgcgag attccttatc tctcttcgcc cgcgagccgg cacgcgcggc
     1681 tggagcgcgt cgtgagctgg tccgcacgca agcagcgcga cgtcaagaag gtgccgcgct
     1741 accacgcgct gcccgagtgg ctcgtgcgcg gcaccgaccc ggtgccgctc ggcgattcgt
     1801 tccgcagcca ggccgcggcg acgcggatcc acgcctttct gatgtcgctg atcgacggcc
     1861 ggcgctcgat caaagacatg gcgaagctcg tcgtcgagca gcggctgatg agcgtcgaag
     1921 aagccgagcc cgcgatccgc tcgttcctca tcaagatgca cgacgacgcg cgccgcggct
     1981 ccacttactg aaccccggcc gtaggcggcc gcgcggcatc gacagctcct tccgagaccg
//
LOCUS       DATLEE010000400         2811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8422274, whole genome shotgun sequence.
ACCESSION   DATLEE010000400 DATLEE010000000
VERSION     DATLEE010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8422274"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..2632
                     /locus_tag="VM692_08575"
     CDS             104..2632
                     /locus_tag="VM692_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52264.1"
                     /translation="MSLTVQADIAADIRYALRGFKRTPGLTLITVLTLAVGLGATAAI
                     FSVVNAVLLRPLPYAEPERLVQVVENVPAAEGFGGVAQRRSAMNATDFEFWRQNTSTL
                     SHVAMLSREGRVLSTDDGSVQLYGQRVSPALFAMRGVQPLLGRGLVAEDETPDNDVVV
                     LADATWRQYFNSAPDILERRIELDGRALTVVGVMPPAFGEDAFWVPFIVPPPGPGVFF
                     SQAEARLADGVSLEAASAEANTLGLQMRGITPEPDAEPRFEVVRSLDQLTVAVAPALR
                     VLVVAVGVVLAIVCTNVANLLLVRGTRRQQEIAIRRSIGATRGRIVRQVLTESLVLAG
                     FSALVAIAFAFAGVALLKLTASSYVNPRFPFAGAVLPRLNDIAVDPTVLAFVVGLALV
                     TGLLFGLLPALRLSKYGERGHASESQLSAMARNSRLGQVLATVQLACAMALLIGAGLL
                     VGSFMKLTGIDSGFDARGVLSFAVVVPGDTTADRKLEIAETLAARLTDDTRVTSAGFA
                     DVPPLTPGIMLMMGNFVPQSMPAAQVAEEQRSLTPEQRTQTRQTSPAYLRALGARLVA
                     GTWLDERASTEPAVLVTRAYADQYFPNGDAVGATLAAGFGSNTVTVAGVVDDVHLGGL
                     ERTPERAVFMDPRLQLTAQRAMVPRPPPQMERNFLTLGGSSITFAARTTGDPLAIMPD
                     LRRIARDIDPRLAIDAAAPMEQVISSLTTRPRFYAVLLGTFGAIAAFIAVIGLYGLLS
                     YVVGQRTKEIGIRMALGAQRDAVLKLVLRQGGAMVAIGLIAGVAGAATLTRYLQGMLF
                     GLEALDPLTFAVVALAFAAVAMVAAYLPARRATTIDPLVAVRYE"
     gene            2661..>2811
                     /locus_tag="VM692_08580"
     CDS             2661..>2811
                     /locus_tag="VM692_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUQ52265.1"
                     /translation="MISDTHGLVRREALAALRGCDAIVHAGDIGKPGVLERLAELAPL
                     TAIRGN"
BASE COUNT          393 a          934 c          980 g          504 t
ORIGIN      
        1 cgaatagggc agtgtgggaa ccgtttggcc agcgccaccg aatatcggtg gcggctaggg
       61 tgtcgattca ctacgatcgt cgacggccac tccaggaggt cgcatgagcc tgactgtgca
      121 agcggatatt gccgccgaca tccgctacgc gctgcgcggc ttcaaacgta ctccgggcct
      181 cacgttgatc accgtgctga cgctcgccgt cggcctcggc gcgacggcgg caatcttcag
      241 cgtcgtcaac gccgtgctgc tgcggccgct gccctatgcg gagcccgagc ggcttgtgca
      301 agtcgtcgag aacgtgccgg cggccgaggg cttcggcggc gtcgcgcagc gccgcagtgc
      361 gatgaacgcg acggacttcg agttctggcg ccaaaacacc agcacgttgt cgcacgtcgc
      421 catgctgtcc cgagaaggcc gcgtgctgtc gacggacgac ggcagcgtgc agctgtacgg
      481 tcagcgcgta tcgccggcgc tgtttgcgat gcgcggcgtg cagccgctgc tcggccgcgg
      541 cttggtggcc gaagacgaaa ccccggataa cgacgtcgtc gtgctcgcgg atgcgacgtg
      601 gcgtcagtac ttcaactccg ctcccgacat cctcgagcgc cgcatcgagc tcgacggccg
      661 cgctctcacg gtcgtcggcg tcatgccgcc ggcattcggc gaagacgcgt tctgggtgcc
      721 gttcatcgtg ccgccgccgg ggcctggcgt gttcttcagc caagcggagg cgcggctcgc
      781 cgacggcgtt tcgctcgagg cggccagtgc cgaagcgaac acgctcggct tgcagatgcg
      841 cggcatcacg cccgagcccg acgccgagcc gcgcttcgag gttgtgcgct cgctggatca
      901 gctgacggtg gcggtcgcgc cggcgttgcg agtgctcgtc gttgctgtcg gcgtcgtgct
      961 cgcgatcgtc tgcacgaacg tcgcgaacct attgctcgtg cgcggtacgc gtcgccagca
     1021 ggaaattgcg attcgccgct cgataggcgc gacgcggggc cgcatcgtgc ggcaggtgtt
     1081 gacggagagt ctcgtgctcg ccggattctc ggcgctggtt gcgatcgctt tcgcgttcgc
     1141 cggcgttgcg ttgttgaagc tgaccgcgtc ctcgtacgtc aatccgcgtt tcccgttcgc
     1201 cggtgccgtg ctaccgcggc tcaacgacat cgccgtcgat ccgacggtgc ttgcgttcgt
     1261 cgtgggcttg gcgctcgtca cgggcctgct attcggtctg ctgccggcct tgcggttgtc
     1321 gaaatacggc gaacgaggcc acgcgtccga gtcgcagctg tccgctatgg cgcgcaactc
     1381 gcggctcggt caggtgctcg ccacggtgca actcgcgtgc gcgatggctc tgctgatcgg
     1441 cgccggtttg ctcgtcggca gcttcatgaa gctcacgggc atcgactccg gcttcgatgc
     1501 acgcggcgtt ttgagcttcg ctgtcgtcgt gcccggggat acgacggccg atcgcaagct
     1561 cgagattgcc gagacgctcg cggcgcgtct gacggacgac acacgagtga cgagcgccgg
     1621 cttcgctgac gtgccgccgc tgacgccggg catcatgctg atgatgggca acttcgttcc
     1681 gcagagcatg ccggcggcgc aggtcgcgga ggagcagcgg tcgctgactc ccgagcagcg
     1741 gacacagaca cggcaaacga gccccgccta tctgcgcgcg ctcggcgcgc ggctagtggc
     1801 gggcacctgg ctcgacgagc gagcaagcac cgagcccgcg gtcctcgtga cccgcgccta
     1861 cgcggatcag tacttcccca atggggacgc cgtcggcgcc acgctggccg ctggtttcgg
     1921 atcgaacacc gtgacggtcg caggtgtcgt cgatgacgtg cacttgggcg gcctcgagcg
     1981 cacacccgag cgcgcggtgt tcatggatcc gcggctgcaa ttgacggcgc agcgcgccat
     2041 ggtgccgcgt ccgccgccgc aaatggagcg taatttcctg acgctcggcg gcagcagtat
     2101 cacattcgcg gcgcgcacca cgggcgatcc actcgcgatc atgccggacc tgcgccgcat
     2161 cgcacgcgac atcgacccgc ggctcgcgat cgacgcggcc gctccgatgg agcaggtcat
     2221 ctcgagcctg acgacgcggc cgcgatttta cgcggtcctc ttgggcacgt tcggcgccat
     2281 tgcggccttc atcgcggtga ttggcctcta cgggctgctg tcgtacgtcg tcggccaacg
     2341 cacgaaggaa atcggcatcc gtatggcgct cggcgcgcag cgcgacgccg tgctgaagct
     2401 cgtgctgcgt caaggcggcg cgatggtggc gattggtctg atcgcgggag ttgcgggtgc
     2461 cgcgacgttg acgcgctatc tgcagggcat gttgttcggg ctcgaagcgc tcgacccgct
     2521 gacgttcgcc gtcgtcgcgt tggcctttgc ggccgtcgcc atggtcgccg cgtacctacc
     2581 ggcgcgccgc gcgacgacga tcgatccgct cgtggccgtg cggtacgaat gagttgccgc
     2641 ggggatccaa gacgctcggt gtgatctcgg acacgcacgg cctcgtgcgg cgcgaggcgc
     2701 tggccgcatt gcgcggctgc gacgccatcg tccacgccgg tgacatcggc aagcccggag
     2761 tgctcgagcg gctcgcggag cttgcgccgc tgacggcgat tcgcggcaac g
//
LOCUS       DATLEE010000401         2886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7675160, whole genome shotgun sequence.
ACCESSION   DATLEE010000401 DATLEE010000000
VERSION     DATLEE010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7675160"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VM692_08585"
     CDS             <1..595
                     /locus_tag="VM692_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HUQ52266.1"
                     /translation="ATFLLTIFVGLTEAIVVGFALGSVLFIHRMAQTTAIAAHVPFAE
                     EPDRADGSNAERRPYDESLATDPDVVVYRITGAFFFGAAAAVGAVLDPIATRHRALIL
                     DLSAVPFLDATAANTLEGLARKAAKRRVRVVLTGANDGVRRELGVHGVTPPHVDYEPS
                     IDAALEQLKNAGLFAATVGRQRKDNEAGDATRSPAPK"
     gene            complement(599..1471)
                     /locus_tag="VM692_08590"
     CDS             complement(599..1471)
                     /locus_tag="VM692_08590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUQ52267.1"
                     /translation="MCEKDHFEEDLEKYIRAGRVTRRDFGSLTAGAGLAMMLPQAVNA
                     QAVTEKDVEIKTPDGTCDAYFVHPSSGAHAAVVVWPDILGLRPAFRQMGKRLAESGYS
                     VLTVNPFYRSARAPVVQPGAAFDEKTRGVVFPMAQKLNATTHASDAKAFIAWLDQQPQ
                     VDKGRKVGTTGYCMGGPITMRTAAAVGDRVGAAASFHGGNLVTQMPDSPHLLVPQIKA
                     KYLFAVAENDDMREPEAKNVLRDAFAKAKLDAEIEVYPAAHGWCPPDSQVYDMAQAEK
                     AWGRMLALFERALA"
     gene            complement(1546..2451)
                     /locus_tag="VM692_08595"
     CDS             complement(1546..2451)
                     /locus_tag="VM692_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ52268.1"
                     /translation="MLELFPDGFRVERRSANGLTLNVRYDATSTPIRPALLLLHGFPQ
                     THAMWHKVADRLSKRFALVIPDLRGYGDSDKPLGSPDHSAYSKRTMALDVLTLMRELG
                     HEQFLVCGHDRGGRVAHRLALDHPQTVRKLVLLDISPTLTMYERTTMEFARRYYHWFF
                     LIQPAPLPERLIGADPSFYLRTKLGALSSEGMAPFDARALAEYERCFADDAAIHAMCE
                     DYRAAATIDLTHDRADAARRIDCPLHVLWGERGVVQHLFTPLEDWQAKCSYEVTGRAL
                     PTGHYIAEEAADLLAAELEAFFALP"
     gene            2547..>2886
                     /locus_tag="VM692_08600"
     CDS             2547..>2886
                     /locus_tag="VM692_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HUQ52269.1"
                     /translation="MSDGVERALVWFRRDLRVEDHAALSHALRAARAVFCVFVFDRNI
                     LAGLPRADRRVEFIRESLVALDESLAAIGGGLIVLDGDPVAEVPRLARELAVAAVYAN
                     HDDEPYARARD"
BASE COUNT          452 a          960 c          982 g          492 t
ORIGIN      
        1 tgcgactttt ttgctgacga tcttcgtggg cttgaccgaa gcgatcgtcg tcggtttcgc
       61 gctcggctcc gtccttttca tacaccgtat ggcgcaaacg acggctatcg ccgcgcacgt
      121 accgttcgcc gaggagcccg atcgcgcgga tgggtcgaac gcggagcgtc gcccttacga
      181 cgagtcgctc gcgaccgacc ccgacgtcgt cgtctaccgg attacgggcg cgttcttctt
      241 tggtgcggcc gccgccgtcg gcgccgttct cgatccgatt gcgacgcgtc atcgcgcgtt
      301 gatcctcgac ctgtcggccg tgccttttct cgacgcgacg gcggcaaaca cgctcgaagg
      361 gctcgcgcgc aaggcggcga agcggcgcgt gcgggtcgtg ctgaccggtg cgaatgacgg
      421 cgttcgccgc gagttgggcg tgcatggcgt aacgccgccg cacgtcgatt acgagccgtc
      481 gatcgacgcg gcgctcgagc agctcaagaa tgccggctta ttcgctgcga cggtcgggcg
      541 tcagcgcaaa gacaacgagg ccggagacgc gacgcggtct ccggccccga agtaagaact
      601 aagcgagagc gcgctcgaac agcgcgagca tgcggcccca cgctttctcg gcctgcgcca
      661 tgtcgtagac ctgcgagtcg ggcgggcacc agccgtgcgc ggccgggtag acctcgatct
      721 ccgcgtcgag cttcgctttc gcgaacgcgt cacgcaacac gttcttcgct tcgggctcgc
      781 gcatgtcgtc gttctcggcc acggcgaaca agtacttcgc cttgatctgg ggcaccaaca
      841 gatgcgggct gtccggcatt tgcgtgacga gattgccgcc gtggaacgag gccgccgcgc
      901 cgacccgatc gccgaccgcg gcggccgtgc gcatcgtgat cgggccgccc atgcagtaac
      961 ccgtcgtgcc gaccttgcgg cccttgtcga cctgcggctg ctgatcgagc cacgcgatga
     1021 acgccttcgc gtcgctggcg tgtgtcgtcg cgttgagctt ctgcgccatc gggaacacga
     1081 cgccgcgcgt cttctcgtcg aaggcggcgc ccggttgcac gaccggcgcg cgcgccgagc
     1141 ggtagaacgg attcacggtc aacacggagt agcccgactc ggcgagccgc ttgcccatct
     1201 gccggaacgc gggccgcagc ccaagaatgt cgggccacac gaccacggcg gcgtgcgcgc
     1261 cgctcgacgg atgaacgaag taggcgtcgc aagtgccgtc cggcgtcttg atctcgacgt
     1321 ccttctcggt cacggcctgc gcgttcacgg cttgcggcaa catcatcgcg agccctgcgc
     1381 ctgccgtgag gctgccgaag tcgcggcgcg tcacgcgccc ggcgcgaatg tatttctcga
     1441 ggtcttcttc gaaatgatct ttctcgcaca tggcgggctt cccctagttg ttgtgattgg
     1501 ccggagagtc ggcaaccggt cgcgtccgac tataccgatt cgccgtcagg gcagtgcaaa
     1561 gaatgcttcg agctcggcgg ccagcaggtc cgccgcttcc tcggcaatgt agtggcccgt
     1621 cggcaaggcg cggcccgtga cctcgtacga gcacttcgct tgccagtctt cgagcggcgt
     1681 gaacaagtgc tgcacgacgc cgcgctcgcc ccagagcacg tgcagcgggc agtcgatgcg
     1741 ccgcgcggcg tccgcacggt cgtgcgtaag gtcgatcgtc gccgcggcac ggtagtcctc
     1801 gcacatcgcg tgaatcgcag cgtcgtcggc gaagcaccgc tcgtactcgg cgagcgcgcg
     1861 cgcgtcgaac ggcgccatgc cttcggagct taaggcgcca agcttcgttc gcaaatagaa
     1921 cgatgggtcc gcgccgatca gccgctccgg cagcggcgcg ggctgaatca ggaagaacca
     1981 gtggtagtag cggcgcgcga actccatcgt cgtgcgctcg tacatcgtca gcgtcggcga
     2041 gatgtcgagc agcacgagct tgcgcacggt ttgcggatga tcgagggcga ggcgatgcgc
     2101 gactcggccg ccgcggtcgt ggccgcagac gagaaactgt tcgtggccga gctcccgcat
     2161 caacgtcagc acgtcgagcg ccatcgtgcg tttgctgtag gccgagtgat cgggggaccc
     2221 aagcggcttg tcggagtcgc cgtagccgcg cagatcggga atcacgagcg cgaagcgctt
     2281 gctcaaacgg tcggcgacct tgtgccacat cgcgtgcgtc tgcggaaagc cgtgcaacag
     2341 caacaacgcg ggccgaatcg gagtcgacgt cgcatcgtag cgcacgttca gagtgaggcc
     2401 gttcgcagag cggcgctcga cgcggaagcc gtcgggaaag agctcgagca tgccgcgagt
     2461 gtggcgccca gggtgcgcgc cgtgcaagat cgacctgaag tgcgcaacga gcccggcaac
     2521 gtgacgaacg gcgaaggacg acgaccgtga gcgacggtgt cgagcgtgcg ctcgtctggt
     2581 ttcgccgcga cctgcgcgtc gaggaccacg ccgcgttatc gcacgcgctg cgcgccgcgc
     2641 gtgccgtgtt ctgcgtgttc gtcttcgacc gcaacatctt ggccgggctg ccgcgcgccg
     2701 atcggcgcgt cgagttcatc cgcgagagcc tcgtcgcgct cgacgagtcg ctcgccgcca
     2761 tcggcggcgg gctcatcgtg ctcgacggcg atcccgttgc cgaggtgccg cggctcgctc
     2821 gtgagctcgc cgtcgcggcc gtctacgcaa accacgacga cgagccctat gcgcgcgcga
     2881 gggact
//
LOCUS       DATLEE010000402         2475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4153922, whole genome shotgun sequence.
ACCESSION   DATLEE010000402 DATLEE010000000
VERSION     DATLEE010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4153922"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VM692_08605"
     CDS             complement(<1..497)
                     /locus_tag="VM692_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUQ52270.1"
                     /translation="MSPKGKRQQSEAWFDRAKKVTPGGVNSPVRAFGSVGGTPLVIRS
                     AMGATVTDSDGREYLDFVGSWGPLILGHAHPKIVAAVEAACRRGTSYGAPCQPEIELA
                     EEVVAAYPGIEQVRFVSSGTEAVMSAVRLARGATGRDLVVKFSGCYHGHVDYLLVAAG
                     SGLVT"
     gene            complement(498..1499)
                     /gene="hemB"
                     /locus_tag="VM692_08610"
     CDS             complement(498..1499)
                     /gene="hemB"
                     /locus_tag="VM692_08610"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HUQ52271.1"
                     /translation="MAELIVTTPGRTRLRRLRRTRALRNLVAETTVGAEQLIMPHFVL
                     PARRANEPISSMPGIAQQGLENVVKQIGKDLEFGIRAVLLFGHPEHGGKTPDGAAAAA
                     ANGAVQKACERLKKEFGDELIVMTDVCLCAYTDHGHCGILENGRVLNDASLAPLVNMA
                     VSHARAGADVVAPSDMMDGRVAAIRDALDDAGLEDTALMSYAVKFASAYYGPFREAAD
                     STPASGDRQSYQMDFRNPREAVREALLDVQEGADWLMVKPALPYLDVIRAVREASLLP
                     LSAYNVSGEYSAVKAAAANGWLDEARIVRENLLAIRRAGADQIITYHARDALHGKWL"
     gene            complement(1487..2218)
                     /locus_tag="VM692_08615"
     CDS             complement(1487..2218)
                     /locus_tag="VM692_08615"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HUQ52272.1"
                     /translation="MKRLAGKRILITRGVDDCAEWAEQLTRYDATPVTLPCIETEIID
                     TPELRAQLAAAVPQTDWLVFTSRRGVEAFAALHEGAIGDARIAAVGPITGEAARLRLG
                     RIDLIGRGGTATQLAATMLTERVLNGRPRLLLALAENADERLETALRDAARCTRFDVY
                     RTIPAPSRAQKRAMSTLGADNVLLASPSAVTGFMNQVELDVAVDIFTIGPSTSAAARA
                     QGLVVTAEAANPSLPGMLEAMLWQN"
     gene            complement(2215..>2475)
                     /locus_tag="VM692_08620"
     CDS             complement(2215..>2475)
                     /locus_tag="VM692_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HUQ52273.1"
                     /translation="QEALARIDHAPSRAAVTAERDALARAEGGCDVAFGAYCFAAGGQ
                     HELTAMHERAGAVRVARVRGTEASQLGAAAWAELVAAHGRKS"
BASE COUNT          362 a          868 c          849 g          396 t
ORIGIN      
        1 aacgtcacga gcccggagcc cgcggcgacg agcaagtagt cgacgtggcc gtggtagcag
       61 cccgaaaact tcacgacgag atcgcggccc gtcgcgccgc gcgcgaggcg cacggcgctc
      121 atgacggctt ccgtgcccga cgacacgaat cgcacttgct cgatgccggg ataggccgcg
      181 acgacttcct cggccagctc gatctcgggc tgacacggtg cgccgtacga cgtgccgcgg
      241 cggcacgcgg cctcgacggc cgcgacgatc ttcggatgcg cgtgacctag gatcagcggc
      301 ccccacgagc cgacgaaatc caagtactcg cggccgtcgg agtccgtgac cgtggcgccc
      361 atggccgaac ggatcacgag cggcgtgccg ccgacgctgc cgaaggcgcg cacgggcgag
      421 tttacgccgc ccggcgtgac cttcttggcg cggtcgaacc acgcttcgct ttgttgtcgc
      481 tttcccttcg ggctcatcta aagccatttc ccgtgcaaag cgtcgcgcgc gtgataagta
      541 atgatctgat ctgcgccggc gcggcgaatc gcgagcaagt tctcgcgcac gatgcgcgcc
      601 tcgtcgagcc agccgttcgc ggcggcggct ttgacggccg agtactcgcc cgacacgttg
      661 taggccgaca acggcagcaa gctcgcttcg cgcacggcgc gaatcacgtc gagatacggc
      721 agcgccggct tgaccatgag ccagtcggcg ccttcttgaa cgtcgagcag ggcttcgcgc
      781 acggcttcgc gcggattgcg aaagtccatc tgatagctct gccgatcgcc gctcgcgggc
      841 gtcgagtcgg ccgcctcgcg gaatgggccg tagtacgccg acgcgaattt aacggcgtac
      901 gacatcagcg ccgtgtcctc gagccccgca tcgtcgagcg cgtcgcgaat cgcggcaacg
      961 cggccgtcca tcatgtcgga cggtgcgaca acgtcggcgc cggcgcgcgc gtggctgacg
     1021 gccatgttga cgagcggcgc gagcgacgcg tcgttcagca cgcggccgtt ctcgaggatc
     1081 ccgcaatggc cgtggtccgt gtatgcgcac aagcagacgt cggtcatgac gatgagctcg
     1141 tcgccaaact ccttcttcaa gcgctcgcag gccttctgca cggcgccgtt ggccgctgcg
     1201 gcagcggcac cgtccggcgt cttgccgccg tgctcgggat ggccgaatag aaggacggct
     1261 cgaatgccga actcgagatc cttgccgatc tgcttgacga cgttctcgag cccttgctgc
     1321 gcgatgccgg gcatcgagga gatcggctcg ttcgcgcgcc gtgccggcag cacgaagtgc
     1381 ggcatgatca attgctcggc gccgaccgtg gtctcggcga cgaggttgcg caacgcgcgt
     1441 gtgcggcgca ggcgccgcaa ccgcgtgcgc ccgggggtcg tgacgatcag ttctgccata
     1501 gcatggcctc cagcattccc ggcaagcttg ggttcgcggc ttcggccgtg acgacgagac
     1561 cttgcgcgcg cgcggccgcc gacgtcgacg gcccgatcgt gaaaatgtcg actgcgacgt
     1621 cgagctcgac ctgattcatg aaccccgtta ccgcggatgg gcttgcgagc agcacattat
     1681 ccgcgccgag tgtcgacatt gcgcgcttct gcgcgcgcga cggcgcggga atcgtccggt
     1741 agacatcgaa gcgcgtgcag cgcgcggcgt ccctcagcgc ggtctcgagc cgctcgtcgg
     1801 cgttctccgc gagcgccagc agtaaccgcg gcctgccgtt caggacgcgt tcggtcagca
     1861 tcgtggccgc gagttgcgtc gccgtgccgc cgcgaccgat cagatcgatg cggccgaggc
     1921 gcaggcgtgc cgcttcgccc gtgatcgggc cgaccgcggc gatgcgcgcg tcgccgattg
     1981 cgccctcgtg cagcgccgcg aacgcttcga ctccgcgtct cgacgtgaac acgagccagt
     2041 cggtctgcgg caccgctgcc gcgagctgcg cgcgcagctc cggcgtgtcg atgatctcgg
     2101 tctcgatgca cggcagcgtg actggcgttg cgtcgtagcg cgtcagctgc tcggcccact
     2161 cggcgcagtc gtcgacgccg cgcgtgatga ggatgcgctt gccggcaagc cgcttcatga
     2221 cttgcggccg tgcgcggcga cgagctcggc ccaggctgcg gcgccgagct gcgacgcctc
     2281 cgtgccgcgc acgcgcgcca cgcgcacggc cccggcgcgc tcgtgcatcg ccgtcagctc
     2341 gtgctgcccg cccgcggcga agcagtacgc gccgaatgcc acgtcgcagc cgccttcggc
     2401 gcgcgcgagc gcgtcgcgct cggccgtcac ggccgcgcga ctcggcgcgt gatcgatgcg
     2461 agccagcgcc tcctg
//
LOCUS       DATLEE010000403         2324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9916526, whole genome shotgun sequence.
ACCESSION   DATLEE010000403 DATLEE010000000
VERSION     DATLEE010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9916526"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1694)
                     /locus_tag="VM692_08625"
     CDS             complement(<1..1694)
                     /locus_tag="VM692_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HUQ52274.1"
                     /translation="MSGAALATRFPRRELLKAGGALLVGFALPLRAQEPQGVALVLGP
                     DQPEQNSLDTWLAIHADNTATVFHGYVELGQGATTALLQIAGDELDLTLDQMRVARLT
                     TGRAPNQGATAASAAIMLGGPRVRAAAAEARQALLRRAAERLGAPVEQLHVTAGVVSV
                     GGQSGTAVSYGELLGDQPFDVPFTGTAPVKPPSDYRLVGARLPRKDIPAKAAGTYEYL
                     QHVRVPGMLHGRVVRPPGQSSCAEGARVATLDETSIRHVAGARVVRRGDFVGVVAPRE
                     WDAVRAARELRIAWETKAALPSSTGVFGQMRAAATIDRVVLETGDAAAAVRSAAHVIA
                     QSYRAPYQAHAPFAPNCALAEARGDEVRVTCGTQNLYETRAKLARVAGVAVENVHVEY
                     RESSGTFGRSCFDDAAEAAVILSQAVGAPVRVQFMRADEHGWDNFGPAQLADVRMASD
                     DAGKLVAYEFEGWQHGWTTVETSEQLALGTAPAEITGPIAMEVSPRNLGSMYAIANRR
                     LVNRRVPGIVGYLKAANLRSPLDIGWSFASEQTIDELAALARLDTYEFRRRNIADER"
     gene            complement(1691..2185)
                     /locus_tag="VM692_08630"
     CDS             complement(1691..2185)
                     /locus_tag="VM692_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HUQ52275.1"
                     /translation="MATPITINERRYDVDASPDTPLLYVLIDELQLRGPRFGCGLAQC
                     GSCSVLVDGVETRSCVTPLAAIGDRQVTTLEGLSAWFGKQRGLAEPPALHPVQQAFID
                     EQAIQCGYCFNGITIKAAELLARNPRPTEAEIRTAMNGHLCRCGTYPRILKAIERAAR
                     ELTP"
BASE COUNT          343 a          831 c          780 g          370 t
ORIGIN      
        1 cgttcgtctg cgatgttgcg gcggcgaaac tcgtaagtgt cgagccgcgc cagcgccgca
       61 agctcgtcga tcgtctgttc cgacgcgaac gaccagccga tgtcgagcgg cgagcgcagg
      121 ttcgcggcct tcagataccc gacgatgccc ggcacgcgcc ggttgacgag ccgccggttc
      181 gcaatcgcgt acatggagcc caagttgcgc ggacttacct ccatcgcgat cggcccggtg
      241 atctcggccg gagccgtgcc gagcgcgagc tgctcggaag tctcgaccgt cgtccagccg
      301 tgctgccaac cttcgaattc gtaggcgacg agcttgccgg cgtcgtcgct agccatacgc
      361 acgtcggcaa gctgcgcagg cccgaagttg tcccagccat gctcgtcggc gcgcatgaac
      421 tgcacgcgca ccggtgcgcc gacggcctgt gacaagatca cggcggcttc cgcggcatcg
      481 tcgaagcaac tgcggccgaa cgtgccggag ctctcgcggt actcgacgtg cacgttttcg
      541 acagccacgc cggccactcg cgcgagcttc gcgcgcgtct cgtagaggtt ttgcgtgccg
      601 cacgtcacgc gcacttcgtc gccgcgcgcc tcggcgagcg cgcagttcgg cgcgaacggc
      661 gcgtgcgctt ggtagggtgc gcggtagctt tgcgcaatga cgtgcgcggc cgagcgaacg
      721 gccgcggccg cgtcgccggt ttcgagcacg acccgatcga tcgtcgctgc cgcgcgcatc
      781 tgtccgaaca ctccggtcga cgacggcagt gccgctttcg tctcccaggc gatgcggagc
      841 tcgcgcgctg cccgcacggc gtcccactcg cgcggcgcga cgacgccgac gaagtcgccg
      901 cgccgcacga cgcgcgcgcc ggcgacgtgg cgaatcgatg tctcgtcgag cgtcgccact
      961 cgcgcgccct cggcgcacga gctctgaccc ggcggccgca cgacgcggcc gtgcagcatg
     1021 ccgggcacgc gtacgtgctg gagatactcg taggtgccgg cggccttcgc cgggatgtcc
     1081 ttgcgcggca ggcgcgcgcc gacgagccgg tagtcgctcg gcggcttcac gggcgcggtg
     1141 cccgtgaacg ggacgtcgaa cggctgatcc ccgagcagct cgccgtagct cacggctgta
     1201 ccgctttgcc cgccgacact gacgacgccg gccgtcacgt gcaattgttc gacgggcgcc
     1261 ccgagccgct ccgcggcacg ccggagcagc gcctgccgcg cttcggccgc ggccgcgcgc
     1321 acgcgcgggc cgccgagcat gatggccgcg ctcgccgcgg tggcgccttg attcggcgcg
     1381 cggcccgtcg tcagccgcgc cactcgcatt tggtcgagcg tgagatccaa ctcgtcaccg
     1441 gcgatttgca gcaacgccgt cgtcgcaccc tggccgagct ccacgtagcc atggaacacc
     1501 gtcgccgtgt tgtccgcgtg aatcgcaagc caagtatcca ggctgttctg ctcgggttgg
     1561 tcgggcccga gcacgagcgc gactccttgc ggctcctgag ctcggagcgg cagcgcaaaa
     1621 cccaccagca acgcaccgcc ggccttcagc aattcgcggc gcgggaaccg agtggccaat
     1681 gcagcgccgc tcatggcgtg agctcgcgag ccgcccgctc gatcgccttc aagatgcgcg
     1741 gatacgtgcc gcaacggcag agatgcccgt tcatcgccgt acgaatctcg gcttcagtcg
     1801 gtcgcggatt ccgcgcgagc aactccgcgg ccttgatcgt gatgccgttg aagcaatagc
     1861 cgcactggat cgcttgctcg tcgatgaatg cctgctgcac ggggtgcagc gccgggggct
     1921 cggcgaggcc tcgctgcttg ccgaaccacg cactcagccc ttccaacgtc gtcacctggc
     1981 gatcgccgat cgccgcgagc ggcgtgacgc aggaccgcgt ctcgacgccg tcgaccagca
     2041 ccgagcacga gccgcattgc gcgagtccgc agccgaagcg cggcccgcgc agttgcagct
     2101 cgtcgatcag aacatacagg agaggcgtat cggggctcgc gtcgacgtcg tagcggcgct
     2161 cgttgatcgt gatcggcgta gccatgtcgc gcctctctag cgccgcagct tgtacgcgag
     2221 cagctctccg gggtattgcg cgccgctgat cgcgacgacg aggtactgct cgccgttcaa
     2281 ctcatatgtc atcggcgagc cgctctgcgg cgccgacatg taca
//
LOCUS       DATLEE010000404         4353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10662592, whole genome shotgun sequence.
ACCESSION   DATLEE010000404 DATLEE010000000
VERSION     DATLEE010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10662592"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..497)
                     /gene="rnpB"
                     /locus_tag="VM692_08635"
     ncRNA           complement(156..497)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VM692_08635"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(533..1375)
                     /gene="rsmI"
                     /locus_tag="VM692_08640"
     CDS             complement(533..1375)
                     /gene="rsmI"
                     /locus_tag="VM692_08640"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HUQ52276.1"
                     /translation="MTDTRESRGKLYVVGTPIGNLDDLSARARDVLAKANIIAAEDTR
                     HTRGLLSRIGVESRLIAYHEHNETERVTALLAELEQGRSVALVSDAGTPLISDPGWRL
                     VAAARAAGIAVIPVPGPCAAIAALSVAGVPTDHFVFEGFLPRRDGARAERLDSLRREQ
                     RTIVFYEAVHRIAETLAALRSAFGGERRAALARELTKTHEQIATGTLAELEARLDSSI
                     PLLGEFVLVVAGAGATSPDEAEAQRVYELLATELAPDKALKLAVAITGVARNTLYRLT
                     RTKN"
     gene            1397..3292
                     /locus_tag="VM692_08645"
     CDS             1397..3292
                     /locus_tag="VM692_08645"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HUQ52277.1"
                     /translation="MNDNQSTSAPRRAARIVTVALLAALTACTSVTPTQRPTGDVAAL
                     EQRARAAAEAGNSAAAADLYTQLASVSRGTVRIDYLLEAARLAADYGDTAMARRRAGE
                     ARNGATPAQQQTAAVLLARLELADGRPQAALDMIAALPQPLLEPAQSDAAAVRGQALF
                     RLGRHVEAVRVLVEREVWLGNADAILANQRMIWEGFRANPSTAPVAPTGDTVVDGWLA
                     LAPLATSAGTDLRRSLLSWRQTYTTHPAAAGLLAELLAAQRSTAFPSQIALLLPLSSP
                     QRAFTLAIRDGFMAAHLRSASSTDTSVRIYDSARLGSTQAYLQAQLDGADFIVGPLFF
                     AEVDQVIAQAGFVPTLALNFATNDTTFLSSFYQFALSPDDEARAAAAAAIAAGAETAL
                     VLAPSNQRGYRIKDNFQAAFEAAGGTMVNWYGYEPALQDFSAPVATLLNVTRSRDRHR
                     RLAANLGVPVQFPEPRRREDVDMIFVAVDPGSDAGRLIAAQLKFFGAGDIPVYANADI
                     FNPGTTARVNDLNGFIFADMPALVAPDDNASSLQADLQAFWPQRNGFVRYYGMGYDAY
                     SLIPSLYANDGRPWSMRGLSGDLSLDMQGRIRRVLPLAQFRNGRPVALDTSLSGAIDS
                     RRLTGAR"
     gene            3292..3654
                     /locus_tag="VM692_08650"
     CDS             3292..3654
                     /locus_tag="VM692_08650"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HUQ52278.1"
                     /translation="MRLERGRLWESRAATYLEEHGLAILARGYRCRLGELDLVCRDDA
                     HLVVVEVRARGRGARCSALDSIDLHKRRRIVQATRHLLMRHAEWQAAAVRFDVVAFDA
                     IDSPEPQIRWVKNAFDAS"
     gene            3709..4290
                     /locus_tag="VM692_08655"
     CDS             3709..4290
                     /locus_tag="VM692_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HUQ52279.1"
                     /translation="MERRVRRHFEDSIATKQAAIVLAPAIAAAAATMTRCLLEDGKIL
                     SCGNGGSAADAQHFSSELLNRFELERPGLPAVALTTDSSTLTSIANDYAFVEVFAKQV
                     RALGQPADVLLAISTSGNSENIVRAIHAAHERGLRVVALTGRDGGAIASLLVDGDIEI
                     RVPAERTARIQEVHLLVIHCLCDLIDAELLEPR"
BASE COUNT          677 a         1500 c         1421 g          755 t
ORIGIN      
        1 ggcgatgtct tctggcggtg agctcgtcga aggcctttca cgagaccgtt cggcgcacag
       61 ggaggtgcgc cgacgagccc cagggacggt cttccggcgt gtctcgggaa cggccctcga
      121 ccggctcgcg gccaggtcga cgaagtgaag taaaaagaag gagtcggcct ataagccggg
      181 ttctgtcgag agcagccatt cctctgcgat gcgcgtcacc gcgcacctgt agcgacctac
      241 ccgggagctc gtgtgggcac acttagcgct cccctattcg gccttgctct gggtggggtt
      301 taccgtgccg cccgtgttac cacgtgcgcg gtgcgctctt accgcaccat ttcaccctta
      361 cctgccattg ctggccggcg gtatattttc tgtggcactt tccgtgggct cgcgcccccc
      421 aggcgttacc tggcaccctg cccatggagc ccggactttc ctccaggtgt taacccagcg
      481 gctgcccggc cgactcccgc cgctagtgta cccgctgcgg cctcgagctc gctcagttct
      541 tcgtgcgcgt caggcgatag agcgtgttgc gcgcgacgcc ggtgatcgcg acggcgagct
      601 tcaacgcttt gtccggggcg agctccgtcg cgagcagctc gtagactcgc tgcgcctcag
      661 cctcgtcggg cgacgtggct cccgcgccgg ccacgacgag cacgaactcg ccaagcagcg
      721 gaatgctcga gtcgagacgc gcttcgagct ctgccaacgt gccggtcgcg atttgttcgt
      781 gcgtcttcgt gagctcgcgt gcaagcgcgg cgcggcgttc gccgccgaac gcgctacgca
      841 gcgcggcgag cgtctcggcg atgcggtgca cggcttcgta gaacacgatc gttcgctgtt
      901 cgcgccgcag cgaatcgagt cgttccgcgc gcgcgccgtc gcgccgcggc aaaaaacctt
      961 cgaacacgaa gtgatcggtc ggcacacctg ctaccgacaa cgcggcgatg gcggcgcacg
     1021 ggccgggaac ggggatcacg gcgataccgg cggctcgcgc ggcggcaacg agccgccatc
     1081 ccggatcgct gatcagcggc gtgccggcgt cgctgacgag cgcgacggac ctgccttgct
     1141 cgagctccgc gagcagcgcc gtaacgcgct ctgtctcgtt atgctcgtga taggcaataa
     1201 gtcgcgattc cacgccgatt ctcgatagca atccccgggt gtggcgcgta tcctcggccg
     1261 caatgatatt tgccttcgca aggacgtcgc gggctcgcgc gctcaagtca tcgagattgc
     1321 cgatcggcgt acccacgacg tagagcttgc cgcgactttc tcgggtatcc gtcatggagg
     1381 ccgaactcgg gtactcatga acgacaacca gtctacaagc gcgccgcgtc gcgcagccag
     1441 aatcgttacc gtcgcattgc tcgcggcgct caccgcatgc acgtcggtga cgcccacgca
     1501 gcgcccgacg ggcgacgtcg ccgcactcga gcagcgcgct cgggccgccg cagaggccgg
     1561 caactccgca gccgccgccg atctttacac gcagttggcg tcggtttcgc gcggcacggt
     1621 gcgcatcgac tatctgttgg aagcggccag gctcgcggcc gactacggcg acactgcgat
     1681 ggcgcgcagg cgtgcaggcg aagcgcgcaa cggcgcaacg cccgcgcagc agcagacggc
     1741 tgcggtgctg ctcgcgcggc tcgaactggc cgacggacgc ccgcaggccg cgctcgacat
     1801 gatcgccgcg ctgccgcaac cgctgctcga gccggcgcag agcgacgctg ccgccgtgcg
     1861 cggccaggcg ctgttccgcc tcggacggca cgtcgaagcg gtgcgcgtgc tcgtcgagcg
     1921 cgaagtctgg ctcggtaatg cggacgccat tcttgcgaac cagcgcatga tctgggaagg
     1981 cttccgcgct aatccttcga cggctcccgt cgcgccgacg ggcgataccg tcgtcgacgg
     2041 ttggctcgcg ctcgcgccgc tcgcgacgag cgccggtacg gatttacgcc gctccttatt
     2101 gtcttggcgg caaacctata ccacgcaccc cgcggccgcc ggtctgcttg ccgagctcct
     2161 ggccgcgcaa cgctcgacgg cgtttccgtc gcagattgcg ctgctgctgc cgctgtcgtc
     2221 gccgcaacgc gcgttcacgc tagcgatccg cgacggcttc atggccgcgc acttgcgtag
     2281 cgccagcagc acggacacga gcgtgcgcat ctacgactcc gcacggctcg gcagcacgca
     2341 agcgtatttg caggctcagc tcgacggcgc cgacttcatc gtcggcccgt tgttcttcgc
     2401 cgaggtcgat caagtcatcg ctcaggccgg cttcgtgccg acgctcgcgc tgaacttcgc
     2461 aaccaacgac acgacgttct tgagcagctt ctatcagttc gcgctctctc ctgacgacga
     2521 agctcgcgcc gccgccgccg ccgcgatcgc agcgggcgca gagacggccc tcgtgctcgc
     2581 gcccagcaac caacgcggct accgaatcaa agacaacttt caagccgcgt tcgaagctgc
     2641 cggcggcacg atggtcaatt ggtacgggta cgagccggcg ttgcaggatt tttcggcgcc
     2701 cgtcgccacg ctgctgaacg tgactcgcag ccgcgaccgg caccgtagac ttgcggcgaa
     2761 tctcggcgtg cccgtgcagt ttcccgagcc gcgacggcgc gaagacgtcg acatgatctt
     2821 cgtcgcggtc gatccgggta gcgacgcggg gcgtctgatc gcggcgcagc tcaagttctt
     2881 cggggccggc gacatcccgg tttacgccaa cgccgacatc ttcaatccgg gaaccacggc
     2941 gcgcgtcaac gacttgaacg gcttcatctt cgccgacatg cccgcgctcg ttgcaccgga
     3001 tgacaacgcg agctcgctgc aagcggatct gcaagccttc tggccgcagc gcaacggctt
     3061 cgtgcgctac tacggcatgg gctacgacgc ctacagcctg atcccgtcct tgtacgcgaa
     3121 cgacggacgg ccgtggtcga tgcgcggcct ctccggcgat ctctcgctcg acatgcaagg
     3181 ccgcatccgc cgcgtgttgc cgctcgcgca gttccgcaac gggcgccccg tcgcgctcga
     3241 cacgtcgctt tccggagcga tcgactcccg caggctcacc ggcgcgcgct gatgcgcctc
     3301 gaacgcggcc gactctggga gtcgcgcgcc gcgacctatc tcgaggagca tgggctcgcg
     3361 atcctggcgc gcggctatcg ctgtcggctt ggcgagctcg atctcgtgtg ccgcgacgac
     3421 gcgcacctcg tcgtcgtcga ggtccgcgct cgcggccgcg gcgcgcgctg ctcggctctc
     3481 gacagcatcg acctgcacaa acgccgccgt atcgtgcaag cgacgcggca cttgctgatg
     3541 cggcatgcgg aatggcaagc ggcggccgtt cgcttcgatg tcgtcgcgtt cgacgccatc
     3601 gactcgccgg agccgcagat tcgctgggtc aagaacgcgt tcgacgccag ttgaaggttg
     3661 ttatcgttcg aggcagagtg atcggcagac ccacgcgcgg cggcacgaat ggaacgacga
     3721 gtacggcgcc atttcgagga cagcatcgcg acgaagcaag ccgcgatcgt gctcgcgccg
     3781 gcgatcgccg ccgcggccgc aacgatgacg cgttgcctgc tcgaggacgg caagatattg
     3841 agctgcggca acggcggctc ggcggccgat gcgcagcact tctcgagcga gctgttgaat
     3901 cgcttcgagc tcgagcggcc cggcttaccc gccgttgcac tgacgacgga cagctcgacg
     3961 ttgacgtcga tcgccaacga ctacgcgttc gtcgaagtgt tcgcgaagca ggtccgcgcg
     4021 ctgggtcagc cggcggacgt tctactcgcg atctcgacgt cgggcaactc ggaaaatatc
     4081 gtgcgagcga tccacgcggc gcacgagcgc ggcttgcgcg tggtcgcgtt gacgggccgt
     4141 gacggcgggg cgatcgcgag cttgctcgtc gacggcgaca tcgagattcg cgtgccggcg
     4201 gagcgtacgg cgcgaattca ggaggttcac ctcctggtca ttcactgtct ctgcgatttg
     4261 atcgacgcgg agttgctaga gcctcgttga gcgcatcgcg ctgtgctccg cgtgctcatg
     4321 ccctgtcgag acacgccgga agaccgtccc tgg
//
LOCUS       DATLEE010000405         3444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5671144, whole genome shotgun sequence.
ACCESSION   DATLEE010000405 DATLEE010000000
VERSION     DATLEE010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5671144"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VM692_08660"
     CDS             complement(<1..966)
                     /locus_tag="VM692_08660"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HUQ52280.1"
                     /translation="MAKVRAPPFRADHVGSLLRPAALKDARRRYTEGALDAAGLAAVE
                     DREIERVAKKQQDMGLLGITDGELRRSWWHLDFLWGLDGVAEHQADFAIGFVGATPRT
                     AGVRVDGRIGFSGHAMLEHYRFLHARVDAVPKLTIPAPSALLGRPVLPPIDARVYPRR
                     EELFDDLGKAYRAAVRAFAAAGCRYLQLDEVFIAMLCDAKYRDKMVARGDDPDALLLR
                     YAALINTAIADAPHDMTVTLHLCRGNFKSTYMGAGGYEAVEDVLFNEIEVDGYFMEYD
                     DERSGGFEPLRFMPRGKKVVLGLVSSKVAKLEDPKQLIRRIEEASK"
     gene            1037..1528
                     /locus_tag="VM692_08665"
     CDS             1037..1528
                     /locus_tag="VM692_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HUQ52281.1"
                     /translation="MIHDVEGDILETKAAAIAHGVAANDPMTQGLAAALHQRYPAMHK
                     DFHHWCRQQHPKLGSAWVWSGAGGVRVIHLITQDGGYEHGSKPQHATLSSVNHALRAL
                     RKLIVKEHLASVALPRLATGVGGLDWAQVRPLIDNQLGDLAAEVYLYREHAAAKKPDE
                     RGH"
     gene            complement(1525..2613)
                     /locus_tag="VM692_08670"
     CDS             complement(1525..2613)
                     /locus_tag="VM692_08670"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HUQ52282.1"
                     /translation="MQTTIRCSLMRGGSSKGLYFRKEDLPADEATRDRVLVAALGSDA
                     RQIDGAGGAHPLTSKVAIVSRSAAGDVDIDYLFVQVVVGEGRVDTTPNCGNILAGVAP
                     FAIENGLVEAREGSTPVRVRMLNSDNLCELLVETPGKRVNYDGDAKIDGVPGTAAPIV
                     CNYLDVAGSATGSLLPTGRVLDVVDGVEVTCIDNGMPVVVLRANDLGVTGYETPKELD
                     ANQALKAKIEAIRLQVGPRMNLGDVTKKVVPKMSLIAPARAGGHVCTRTFIPHDCHAA
                     IGVLGAVSVATACILPGSATHGIATVPDGSTKQMSVEHPTGEFSVTLEVGGTRDKPTV
                     LKAGLLRTARLIMRGEVLVPEAVWNGRR"
     gene            2660..>3444
                     /locus_tag="VM692_08675"
     CDS             2660..>3444
                     /locus_tag="VM692_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUQ52283.1"
                     /translation="MKRPLGNTGSSVVPIGLGGMPLSIQGRPDERAAHDVIAAFVEGG
                     GNFIDTAISYCLDDSEFGHNERLIAKSLRALKRTDVIVATKGGLTRPNGRWEVDCSPD
                     WLRRNCEQSVRALGGPIALYYLHTVDPAVPLVDSVGELVRLRGEGKIAAIGLSNVDAR
                     QLDEALRLTPIAAVENRCNVFDRRDFDNGLVDRCRELGIAYVPYSPVGGHFRHNRVGD
                     DATLTRIAAKHATTSYVIALAWLLAKGAHVLPIPGATKTASIR"
BASE COUNT          587 a         1197 c         1105 g          555 t
ORIGIN      
        1 cttcgaggct tcttcaatgc gcctgatgag ttgcttcggg tcctcgagct tcgcgacctt
       61 gctcgacacg aggcccagca ccaccttctt gccgcgaggc atgaagcgca gcggctcgaa
      121 gccgcccgag cgctcgtcgt cgtactccat gaaatagccg tcgacttcga tctcgttgaa
      181 aagcacgtcc tcgacggcct cgtagccgcc ggcgcccatg tacgtcgatt tgaagttgcc
      241 gcggcacaga tgcagcgtca ccgtcatgtc gtgcggagcg tcggcgatcg cggtgttgat
      301 caacgccgcg taccgcagca ataacgcatc gggatcgtca ccgcgcgcga ccatcttgtc
      361 gcgatacttc gcgtcgcaga gcatcgcgat gaagacttcg tcgagctgta ggtagcggca
      421 gccggcggcc gcgaaggcac ggacggccgc gcgatacgcc ttgccgaggt cgtcgaacag
      481 ctcctcgcgc cgcggataga cgcgcgcgtc gatcggcggc aacacgggcc ggccgagcag
      541 cgccgaaggc gcgggaatcg tcagcttcgg tactgcgtcg acccgcgcgt gcaggaaccg
      601 gtagtgctcc agcatcgcgt gccccgagaa gccgatccgg ccgtcgacgc gcacgccggc
      661 cgtgcgcggc gtcgcgccga cgaagccgat cgcgaaatcg gcttggtgct cggcgacgcc
      721 gtcgagcccc cagaggaaat cgaggtgcca ccacgagcgc cgcaattcgc cgtccgtgat
      781 gccgagcaag cccatgtctt gctgcttctt ggcgacgcgc tcgatctcgc ggtcctcgac
      841 ggccgcgagg ccggccgcat cgagtgcgcc ctcggtgtat cgccggcgcg catccttgag
      901 tgcggcgggg cgcaacaagc tgccgacgtg atcggcgcga aacggcggcg ctctaacctt
      961 ggccatcggc aagctctttg agcgaagata gcctagagcg taggcgagtc ttcacccgag
     1021 cgcgaggtga gcacgcatga tccacgacgt cgaaggcgac atcctcgaga cgaaagccgc
     1081 agcgatcgcg cacggcgtcg ccgccaacga tccgatgact cagggcctcg cggccgcgct
     1141 gcaccaacgc tatccggcca tgcacaagga cttccaccat tggtgtcgcc agcaacaccc
     1201 gaagttgggc agtgcttggg tgtggagcgg cgccggcggc gtgcgcgtca tccacttgat
     1261 cacgcaggac ggcggctacg agcacggcag caagccgcag cacgcgacgt tgagcagcgt
     1321 caaccacgcg ctgcgcgcgc tacgcaagct catcgtgaag gagcacctcg cgagtgtcgc
     1381 gctaccgcgg ctcgcgacgg gcgtcggcgg tctcgactgg gcccaagtcc ggccgttgat
     1441 cgacaatcag ctcggcgacc ttgcagccga agtctatctc taccgcgagc acgcggccgc
     1501 gaagaagccc gacgagcgcg ggcactagcg gcggccgttc cagacggcct cgggcacgag
     1561 cacctcgccg cgcatgatga ggcgcgcggt tcgcagcaaa cccgctttca gcaccgtcgg
     1621 cttgtctcgt gtgccgccga cctcgagagt caccgagaac tcgcccgtcg gatgctcgac
     1681 cgacatctgc ttcgtcgatc catcgggaac cgtcgcgatg ccgtgcgtcg cggagcccgg
     1741 caggatgcac gccgtcgcga cggaaacggc gccgagcacg ccgatcgctg cgtgacagtc
     1801 gtgcgggatg aacgtgcgcg tgcagacgtg gccgccggcg cgcgccggcg cgatcaagct
     1861 catcttcggc accactttct tcgtcacgtc gccgagattc atgcgcggcc cgacttgcaa
     1921 ccggatcgcc tcgatcttcg ccttcagcgc ttggttcgcg tcgagctcct tcggcgtctc
     1981 ataacccgtg acgcctagat cgttcgcacg cagcacgacg acgggcatgc cgttgtcgat
     2041 gcatgtcacc tcgacaccgt cgacgacgtc gagcactcgg cccgtcggca acaagctgcc
     2101 cgtcgccgag cctgcgacgt cgagataatt gcagacgatc ggcgccgcgg ttcccggcac
     2161 gccgtcgatc ttggcgtcgc cgtcgtagtt gacgcgcttg ccgggcgtct cgaccaatag
     2221 ctcgcaaaga ttgtcgctgt tcaacatccg cacgcgcacc ggcgtgctgc cttcgcgggc
     2281 ctcgacgagg ccgttctcga tcgcgaacgg cgcgacgccg gccaagatgt tgccgcagtt
     2341 cggcgtcgtg tcgacgcgcc cctcgccgac gacgacctgg acgaacagat aatcgatgtc
     2401 gacatcgccc gctgccgagc ggctcacgat cgcgaccttg ctcgtcagcg gatgcgcgcc
     2461 gccggcgccg tcgatctggc gcgcgtcgct gcccaaggcc gcgacgagca cgcgatcgcg
     2521 cgtcgcctcg tcggcgggca gatcttcctt gcggaaatac agacccttcg agctgccgcc
     2581 gcgcatcaag gaacatcgga tcgtcgtttg catgcacgta tggtacggcg cgccgcggac
     2641 cggctgctac actgcatgca tgaagcgacc actcggcaac acgggctcga gcgtcgtgcc
     2701 gatcggcctc ggcggcatgc cgctgtccat ccagggccga ccggatgagc gagccgcgca
     2761 cgacgtgatc gccgcgttcg tggaaggcgg cggcaacttc atcgacacgg caatcagcta
     2821 ctgcctcgac gactccgagt tcggccacaa cgaacggctg atcgccaaat cgctgcgcgc
     2881 tttaaaacgc accgacgtca tcgtcgcgac gaagggcggc ctcacgcggc ccaacggtcg
     2941 ctgggaagtc gactgcagcc cggactggtt acgccgcaac tgcgagcaaa gcgtgcgcgc
     3001 tctcggcggg ccgattgcgc tctattactt acacaccgtg gacccggccg tgccgctcgt
     3061 ggattcggtc ggcgagctcg tgcggctgcg cggcgagggc aagatcgccg caattggcct
     3121 ctcgaacgtc gacgcgcggc aactcgacga ggccttgcgc ttgacgccga tcgccgccgt
     3181 ggagaaccgc tgcaacgtgt tcgaccgacg cgatttcgac aacggtctcg tcgaccgctg
     3241 ccgagagctc ggcatcgctt atgtccccta ttcgccggtc ggcggccatt ttcgccacaa
     3301 ccgcgtcggc gacgacgcga cgctgacgcg catcgcggcg aagcacgcca cgacttcgta
     3361 tgtcatcgcg ctcgcttggc tgctcgccaa gggcgcccac gtgctaccga ttccaggcgc
     3421 taccaagacc gcgagcatcc gcag
//
LOCUS       DATLEE010000406         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4161340, whole genome shotgun sequence.
ACCESSION   DATLEE010000406 DATLEE010000000
VERSION     DATLEE010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4161340"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VM692_08680"
     CDS             <1..327
                     /locus_tag="VM692_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HUQ52284.1"
                     /translation="VDQECMTITYANGPDVRFNFDYYVNTHMPRIMSLYGKSISRFEL
                     RRGLPGADGAPPPFVATITIWIADGKAFDEAQVKHQAGLRADVPKFTNAVLAAQRDRI
                     VGTASS"
     gene            737..1147
                     /locus_tag="VM692_08685"
     CDS             737..1147
                     /locus_tag="VM692_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HUQ52285.1"
                     /translation="MFILKPHSGVPIYRQLSEQIRRMAASGQLLPGTELPSIRELALT
                     HAINPMTVSKVYTLLEAEGVLERHRGKPMTVAKPQRTQSPIAKRLEQLEPHVEQVALA
                     ARQLQLTEKDAVKALHDAWEKTDVESVHRRARPR"
     gene            1110..1988
                     /locus_tag="VM692_08690"
     CDS             1110..1988
                     /locus_tag="VM692_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ52286.1"
                     /translation="MLSPYIAARGLGKSFNDKPVLVDVSFTVEPGDVVGVLGKNGAGK
                     TTLLELVLGFTPATSGRVQLFGYDSYRLPGAAKARIGFVPQQDELVNQLTAADQIGVI
                     ASFYPKWDDELVGRLSREWEVDLNQRVKNMSAGQRQKLSILLGLGHRPDVLILDEPVA
                     SLDPVARRQFLEQIIEVASDGNRSVVFSSHIVSDVERLANKIWILKDQRMYWQGDFDS
                     LKDSIVRLHARSTQPVPASFAIPNALSLQRNGTTATAVVQGWRAEMHDDLARQLEAEI
                     EIEQLTLEDIFLELHR"
BASE COUNT          376 a          703 c          654 g          348 t
ORIGIN      
        1 gtcgatcagg agtgcatgac gatcacgtac gcgaacggcc ccgacgtgcg cttcaacttc
       61 gactactacg tcaacacgca catgccgcgg atcatgagcc tctacggcaa gtcgatcagc
      121 cgcttcgagc tgcgccgcgg cttaccgggc gccgacggcg cgccgccgcc gttcgtcgcg
      181 acgatcacga tctggatcgc cgacggtaag gcgttcgacg aggcgcaagt gaagcatcaa
      241 gccgggttgc gcgccgacgt gccgaaattc acgaacgccg tgctggcggc gcagcgcgac
      301 cgcatcgtcg gcaccgcctc ttcttaatag agagagcgcg atctcctgag aactgccgca
      361 cacgctgcgg cggctcccga ccgttctccc gccgaactgc gcactcggcc acagaaccgc
      421 ggctcgcggc gcgcctagcc tgcgctcacc ggactaccgt gggcgcaccg cttgcacgca
      481 ccgaccgccg tcgtcgaccg acgattcatg gcctacgtca cggacttgct ggtctgcagc
      541 cgctgcctgc aggccggcgt tcggacgccc gtgaggcaag tcatctcgcc gcgccatcgg
      601 ccgcgcgcgc tctgcgggca gtgccggcga cataacgctg ccgctcgcta gctcgtccct
      661 gcacttccaa ctgcgtcatt gacttcaaac ggtctacgtg cattattgca ctaatgcact
      721 ttgcggaccg cgacgcttgt tcattctgaa gccgcactcg ggcgtgccga tctaccggca
      781 gctctcggag cagatccgac gtatggccgc gagcggacaa ctgctgccgg gtacggagct
      841 gccgtcgatc cgcgagctcg cgctcacgca cgcaatcaat ccgatgacgg tgtccaaggt
      901 ctacacgctg ctcgaagcgg aaggcgtgct cgagcgccac cgcggcaaac cgatgaccgt
      961 ggcgaagccg caacgcacgc agtcgcctat cgcgaagcga ttggagcagc tcgagccgca
     1021 cgtcgagcag gtcgccctcg ccgctcggca gctacagctc accgaaaaag acgcagtgaa
     1081 agccttgcac gatgcatggg agaagaccga tgttgagtcc gtacatcgcc gcgcgcggcc
     1141 tcggtaagtc gttcaacgac aagccggttc tcgtcgacgt ctcgttcacc gtcgagcccg
     1201 gcgacgtcgt cggcgtgctc ggcaagaacg gtgccggcaa gacgacgctg ctagagctcg
     1261 tgctcggctt cacgcccgcg acctcggggc gtgtccagtt attcggctac gacagctacc
     1321 ggctgcccgg cgcggcgaaa gcacgcatcg gcttcgtgcc gcagcaggac gagctcgtga
     1381 accagctgac ggcggcggac cagatcggcg tcatcgcttc gttctatccg aagtgggacg
     1441 acgagctcgt tggccgcttg agccgcgagt gggaagtcga tctcaaccag cgcgtcaaga
     1501 acatgtcggc cggccagcgg cagaagctgt cgatcctgct cgggctcggg cacaggcccg
     1561 acgtgctgat cctcgatgaa cctgtcgcga gcctcgaccc cgtcgcgcgc cgccagttcc
     1621 tcgagcaaat catcgaagtc gcgtccgacg gcaaccggtc ggtggtgttc tcgtcgcaca
     1681 tcgtttccga cgtcgagcgg ctcgcgaaca agatctggat cctcaaggat caacgcatgt
     1741 attggcaggg cgacttcgac tcgctgaagg attccatcgt tcggctacac gcgcgctcga
     1801 cgcaaccggt gccggcctcg ttcgcgattc cgaacgcgct cagcctgcag cgcaacggca
     1861 cgacggcgac cgcggtcgtg caaggctggc gggccgagat gcacgatgac ctcgcgcggc
     1921 agctcgaagc cgagatcgag atcgagcagc tcacgctcga ggacatcttc ctggagctgc
     1981 atcggtgaag gcgatcgtgc ggttgatcta cacgaacttc acgggcacgg cactgctgcg
     2041 cgtaatcacg ccggtggggc tgctcgtggg gatcctcggg t
//
LOCUS       DATLEE010000407         6551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4927602, whole genome shotgun sequence.
ACCESSION   DATLEE010000407 DATLEE010000000
VERSION     DATLEE010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4927602"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..701)
                     /locus_tag="VM692_08695"
     CDS             complement(165..701)
                     /locus_tag="VM692_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUQ52287.1"
                     /translation="MPEPADDLTESAPGRPLSAWRFRLAVLLHDRVVYRVAAALLRDA
                     REAEDVTQEVFLSYWQHGATVERPREWLLKVARNAALGRLRKSGRLISHEQQELPEPS
                     DRRDPSWHYEQNELAGELRKLVDTLPEPQRSLLVMFDVQGHDGETCARVLGLNTNQVK
                     VYLHRARRKLREQLERAS"
     gene            812..2911
                     /locus_tag="VM692_08700"
     CDS             812..2911
                     /locus_tag="VM692_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HUQ52288.1"
                     /translation="MRKALALLSICVAPAALASHDESLGARFVQSGGVDAGNCLDHHA
                     PCLSIRYALEQAQPGNTVKVGAGIFDLRGVDPNRYLHGPVHATGGYDELDHYLESRPS
                     AVQSIVTGVDPRYRHALAVRGFHWAESVAAARRGVVSFDGPALQASASAPAACTQGFA
                     GAFPCRNIAFQSQIPLAQFSSAPVSAANVWGFVDRNDNREYAVLGLRNGTAIVDVTDP
                     LNPREVVTIPGNSSPWREVKVYQAFDSAANRWRAYAYVSTEAANSGVQTIDLSGLPQT
                     AVLASTNLDTSSQHTLYVSNIDYSTNSALPGLTPVLYVAGSNLAGGSWRAYSLANPAS
                     PQLLSSAPTTHYMHDSTGLVLTDSRTTQCAAGHNPCEILADFDVDQVELWDVTSKLQP
                     VLLGTATNPNNRYIHSGWPNINNRFILFHDELEEIQFGQNTRIYTLDLTNGLSSPNVT
                     VSYQGPTTTTDHNGYIHETRYYVSHYRRGVVVFDASDPTRLVEIGHFDNYLTPAANTA
                     GTDGAWGVYPFLPSRNLLVSDIENGLFVLRNFTHNLDQANGRLGFGELAATTTESAGV
                     VSVRVQRTLGRLGAVSVQYSTSGGSASEGSDYTAASGTLNWANQDIADKVIAIPVFDD
                     TSVEGAETLSLTLSNLSGFATLDGSSTLTITIADNDSTPPASGGGGRSGGGSMDLALL
                     ALLASTLLNKVPRRRRA"
     gene            complement(2908..4209)
                     /locus_tag="VM692_08705"
     CDS             complement(2908..4209)
                     /locus_tag="VM692_08705"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HUQ52289.1"
                     /translation="MEGLFSADRLIAMWNTVYGWLLTNVLVLDNAVQLAIAGLTLLLA
                     LLFAQHFKPWLARYRDHRSVGRGVMIAESMTLSVVWLALLWLAMAIVSGSDRPSGLLS
                     TALTLLAAWIVIHFGSQFVRNRTLARLITWAVWSIAALSILGLLQPIIATLDSAAIQI
                     GDLRVSIYTVVRSTVSLAVLLYVAVYLTGLTEARIRTSQTLSPTVKVLLTKSLKIVLI
                     SIAVLVAFASVGIDLGALAVVGGALGLGVGFGLQKIVSNLISGVILLLDKSIKPGDII
                     AVSGTYGWVTALGGRYVSVVTRDGVEHLIPNETLISESVENWTHTQRRTRLKLDLFVH
                     HDSDLKQVIALCEAAARETERILPEPAPKCLFIEIGESSLKLQLRFWIGDAQNGVQNV
                     KSDVLLEIWQRFKQQGIRVPFPQRDVHVRSSAERPTLQALA"
     gene            4317..5594
                     /locus_tag="VM692_08710"
     CDS             4317..5594
                     /locus_tag="VM692_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ52290.1"
                     /translation="MTKGRVLFASLIGTTIEFFDFYIYGTAAVLVFPQLFFPTGNATT
                     AMLQSLATFALAFFARPVGSALFGHFGDRIGRKATLVAALLTMGLSTVAIGLLPTYAT
                     IGIAAPALLALCRFGQGLGLGGEWGGAVLLATENAPRGKESWYGMFPQLGAPLGFFCS
                     TGIFIALTASFSDADFLAWGWRIPFLASAVLVGVGLWVRLSLTETPAFQRAIEREERV
                     RVPLLVVAGEHARTLVLATLAAITTFVVFYLMTVFTLSWGTTALGFSRQEFLPLQMIG
                     VLFFAATIPLTALAADRVGRWPVLIASSIAAIVFGLVFEPLFSSGDAVTAAVFLSLGL
                     ALMGCTYGALGSALAELFPTAIRYTGASLTFNLAGIFGGSLAPYIATYLAASHGIAYV
                     GYYLSAAAALTLTAQLMLNRRQAPHPASERSAA"
     gene            complement(5539..6189)
                     /locus_tag="VM692_08715"
     CDS             complement(5539..6189)
                     /locus_tag="VM692_08715"
                     /inference="COORDINATES: protein motif:HMM:NF040011.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HUQ52291.1"
                     /translation="MEQGTDLALRCRCGTVRGIARGVTPSSINHCFCYCDDCQAFAHF
                     LGRADEVLDAHGGTEITQMSQATVRFAAGVDKVAAMRLSPKGMIRWYASCCRTPIGNT
                     MPTSAMPFIGVIKAFIDAPSAALGPIRGRGFKNSAKGGPSAVPKDGLPELVMIARVLA
                     KVLGWRLRGDHRRSALRNATTAKPLVEPHVLEATEREDLRRRCARWRGEAPAGGST"
     gene            complement(6219..>6551)
                     /locus_tag="VM692_08720"
     CDS             complement(6219..>6551)
                     /locus_tag="VM692_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ52292.1"
                     /translation="ASYNPFTSLQWFLDGKTVAGTAIRGPEETPSRLQALKFYTLGSA
                     WFSFDEDVRGSLEVGKLADLAVLSQDYLTVPLDEVGNIESVLTLLGGEIVYAAGDLAA
                     LEQPAGSH"
BASE COUNT         1084 a         2261 c         2089 g         1117 t
ORIGIN      
        1 gtgcaaggcg cgacgcacgg cgacggcgcg ctccgccgcg gcggcgagtc gtgggtcggc
       61 cgcaatcgcg gcgctcatgc gcgcccggcc ctcgcccgtc aggctgccgt cggcccaggc
      121 ttcgagctgc gtgcgcgccc actccgtgtt gagatctcgt tccgtcatga cgctcgctcc
      181 agctgctcgc gcaacttacg ccgtgcgcgg tgcaggtaaa ccttgacctg attcgtgttg
      241 agcccgagca cacgcgcgca cgtctcgccg tcgtggccct gcacgtcgaa catcacgagc
      301 aggctgcgct gcggctccgg caacgtgtcg acgagcttgc gcagctcgcc ggccagctca
      361 ttttgctcgt agtgccacga gggatcgcgg cggtcgctcg gctccggcaa ctcttgctgc
      421 tcgtggctga tgagccgacc cgacttacgc aggcgaccga gcgccgcatt gcgagcgact
      481 ttcaacagcc attcgcgcgg ccgctcgacc gtcgcgccat gttgccagta gctcaagaac
      541 acctcttgtg tcacgtcttc ggcctcgcgt gcatccctga gcaaggcggc ggccacacgg
      601 tagacgactc gatcgtgcag cagcaccgcg agccgaaagc gccacgccga cagcgggcgg
      661 cccggcgccg attccgttaa atcgtcggcc ggctcgggca tcgcggatcg ctccgattgt
      721 cgaaccggct gcagcgccat acccttatcc gttgaaccac aacgaccaag acggcccacc
      781 cagtcgaagg ttacaccgcg aggaccgccg catgcgtaag gccctggcgc tcctgtctat
      841 ctgcgtcgca cccgctgctc tggcgtcgca cgacgagtcg ctcggcgcgc ggttcgtgca
      901 gtcgggcggc gtcgacgccg gcaactgcct cgatcaccat gcgccttgct tgtccattcg
      961 ctatgcgctc gagcaagcgc agccgggcaa cacggtaaag gtcggggccg gcatcttcga
     1021 tttgcgcggc gtcgatccaa accgctatct acacgggccc gtgcacgcga caggcggtta
     1081 cgacgagctc gatcactacc tcgagtcgcg cccgtccgcc gtgcagtcga ttgtcacggg
     1141 cgtcgacccg cgttatcggc atgccctggc cgtgcgcggg ttccactggg ccgagagcgt
     1201 cgcagctgcg cggcgcggcg tcgtctcgtt cgacggtccc gcgctgcaag cgtccgcctc
     1261 ggcgcctgcc gcgtgcacgc aaggattcgc cggcgccttc ccgtgccgca acatcgcctt
     1321 ccagtcgcag atcccgctcg cgcagttttc gagcgcgccc gtctcggcgg cgaatgtttg
     1381 gggcttcgtc gaccgcaacg ataaccgcga gtacgcggtg ctcggactgc gcaacggcac
     1441 ggcgatcgtc gacgtaacgg accccctcaa cccgcgcgag gtcgtcacca tcccgggcaa
     1501 cagctcgccg tggcgcgagg tgaaggtcta ccaggcattc gatagcgcgg cgaatcgctg
     1561 gcgcgcgtac gcctatgtca gcaccgaggc cgcgaacagc ggcgtgcaga cgatcgacct
     1621 gtcggggctg ccgcagacgg ctgtgctcgc gtcgacgaat ctcgacacga gctcgcagca
     1681 cacgttgtac gtctcgaaca tcgactattc gacgaactcc gcgctgcctg gcctgacgcc
     1741 ggtcctctac gtcgcgggca gcaatctcgc gggcggctcc tggcgcgcgt acagcctcgc
     1801 gaatccggcg tcaccgcaat tgctgagcag cgcaccgacg acgcactaca tgcacgactc
     1861 gacgggtttg gttttgacgg acagccgcac gacgcagtgc gcggccgggc acaacccctg
     1921 cgagatactc gccgacttcg acgtcgacca agtcgagctc tgggatgtca cgagcaagct
     1981 tcagcccgtg ctgctcggca cggcaacgaa cccgaacaat cgctacatcc actccggctg
     2041 gccgaacatc aacaatcgtt tcatcctgtt ccacgacgag ctcgaggaaa tccaattcgg
     2101 ccagaacacg cgcatttaca cgctcgacct gacgaacggc ctctcgtccc cgaacgtgac
     2161 cgtcagctat caaggtccca cgacgacgac cgatcacaac ggctacatcc atgaaacgcg
     2221 ctactacgtg tcgcactatc ggcgcggagt ggtcgtgttc gacgcgagcg acccgacgcg
     2281 gctcgtcgag atcgggcact tcgacaacta cctgacaccg gctgcgaata ccgccggcac
     2341 cgacggtgcc tggggcgtct atccgttcct gccgtcgcgc aaccttctcg tcagcgacat
     2401 cgagaacggc ctgttcgtcc tgcgtaactt cacgcacaac ctcgaccagg ccaatggccg
     2461 cctcgggttt ggcgaactcg cggcgacaac caccgagagc gccggtgtcg tcagcgtccg
     2521 ggtgcaacgg acgctcgggc gcctcggcgc cgtcagcgtt cagtactcga cgagcggcgg
     2581 gtcggcgagc gaaggcagcg actacacggc cgcgagcggg acgctcaatt gggccaacca
     2641 ggacatcgcg gacaaagtca ttgcgattcc tgttttcgat gacacgagtg tcgaaggcgc
     2701 cgaaacgctg agcttgaccc tgtcgaacct gagcggcttt gcgacgctcg acggctcgag
     2761 cacgttgacg atcacgatcg ccgacaacga ctcgacgcca cccgcgagcg ggggcggcgg
     2821 cagaagcggc ggcggctcga tggacctcgc attgctcgcc ctgcttgcga gcacgctgct
     2881 caacaaagtg ccgcgccgtc gccgggctta ggccagcgcc tgcaaggtcg ggcgctcggc
     2941 gctcgagcgc acgtgcacat cgcgctgcgg aaacggcacg cgtatgcctt gctgcttgaa
     3001 gcgctgccag atctcgagca gcacgtcgct cttgacgttc tgcacgccgt tctgcgcgtc
     3061 gccgatccag aaccggagct gcagcttcag cgagctctcg ccgatttcga tgaagagaca
     3121 cttcggcgcc ggctccggca gaatgcgctc cgtctcgcgc gccgccgcct cgcagagcgc
     3181 gatgacttgc ttcaagtcgg agtcgtggtg cacgaacaag tcgagcttga gccgcgtgcg
     3241 ccgctgcgtg tgcgtccagt tctccaccga ctcggagatc agagtctcgt tcggaatcaa
     3301 gtgctcgacg ccatcccgcg tcacgaccga gacgtagcgg ccgccgagcg ccgtcaccca
     3361 cccgtaggtt ccagacaccg cgatgatgtc gccgggtttg atcgacttgt cgaggagcag
     3421 aatgacgccg ctgatcaggt tgctgacgat cttctgcaag ccgaagccga cacccaaacc
     3481 gagcgcgccg ccgacgactg ccaaggcgcc gaggtcgatg ccgacgctgg cgaacgcgac
     3541 gagtaccgcg atcgagatca gcacgatctt caacgacttc gtcaacagca ccttcacggt
     3601 cggcgacagc gtctgactcg tgcggatgcg cgcttccgtc aagcccgtga ggtacaccgc
     3661 gacgtacagc agcaccgcga gcgacaccgt cgagcgcacg actgtgtaga tcgagactct
     3721 gagatcgcca atctggatcg cggcggagtc tagggtggcg atgatcggct ggagcagccc
     3781 gagaatgctc agagccgcaa tcgaccaaac cgcccaggtg atcagcctgg ccagcgtacg
     3841 gttgcggacg aattgcgagc cgaagtggat gacgatccaa gctgcgagca gcgtgagcgc
     3901 ggtgctcaag agccccgacg gccggtccga tccgctcacg atggccattg cgagccacag
     3961 caacgcgagc cacaccacgg acagcgtcat cgattcggcg atcatgaccc ctcggcccac
     4021 gctgcggtgg tcgcgatagc gcgcgagcca aggcttgaag tgctgcgcga acaacagcgc
     4081 gagcagcagc gtcaagccgg cgatcgcgag ttgcaccgcg ttatcgagca cgagcacgtt
     4141 cgtgagcagc cacccgtaaa cggtgttcca catagcgatc aggcggtctg ccgagaatag
     4201 cccttccatt tgcgtggcct ccgcaggttg aggtgaaacc tcagtctaag cccgccggcg
     4261 ccgcgggccg aaagtgtcgc cggcgcggca cgctcgccga tataatcccg gccccaatga
     4321 ccaagggccg cgtgctgttt gcgagcttga tcggcacgac gatcgagttc ttcgatttct
     4381 acatctacgg cacggccgcc gtcctcgtat tcccgcagct gttcttcccg accggcaacg
     4441 cgaccacggc gatgctgcag tcgctcgcga cgttcgctct ggcgttcttc gcgcgccccg
     4501 tcggctccgc gctgttcggc cacttcggcg accgcatcgg ccgcaaggcg acgctcgtcg
     4561 ccgcgctgct gaccatgggg ctgtcgacgg tggccatcgg cctgttgccg acgtacgcga
     4621 cgatcggaat cgcagcgcct gcgttgctcg cgctctgccg cttcggtcag ggcttagggc
     4681 tcggcggcga atggggcggc gccgtgctgc tcgcaaccga gaacgcgccg cgcggcaagg
     4741 agagctggta cggcatgttc cctcagctcg gcgctccgct cggcttcttc tgctcgacgg
     4801 gaatcttcat tgcgttgacg gcgagcttca gcgacgccga ttttcttgcg tggggctggc
     4861 gcattccgtt cctcgcgagc gcggtgctcg tcggcgtcgg gctctgggtg cggctgtcgc
     4921 tgacggaaac gccggcgttc caacgcgcga tcgagcgcga ggagcgcgtc cgcgtgccgc
     4981 tcctcgtcgt cgccggcgag cacgcgcgca cgctcgtgct cgccacgctc gcggcgatta
     5041 cgacgttcgt cgtgttctac ctgatgacgg tgttcacctt gagctggggt accacggcgc
     5101 tcggcttcag ccgccaggag ttcctgccgc tgcagatgat cggcgtgctg ttcttcgcgg
     5161 cgacgatccc gttgacggcg ctcgccgcgg atcgcgtcgg ccgctggccg gtgctgatcg
     5221 cgtcgagcat cgcggcgatc gtcttcggcc ttgttttcga gccgctgttc tcgagcggcg
     5281 atgccgtgac ggcagccgtg ttcttgtcgc tcgggctcgc gttgatgggc tgcacgtacg
     5341 gcgcgctcgg ctcggccctc gccgagctgt ttccgacggc gattcgctac acgggcgcgt
     5401 cgttgacgtt caacctcgca ggaattttcg gcggctcatt ggcgccgtac attgcgacct
     5461 acttggccgc gtcgcacggc atcgcgtacg tcggctatta cttgtcggct gccgccgcgc
     5521 tgaccctgac cgcgcagctc atgttgaacc gccggcaggc gcctcacccc gccagcgagc
     5581 gcagcgccgc ctgagatcct cgcgctcggt cgcctcgagc acatgcggct cgacgagcgg
     5641 cttggccgtc gtcgcgttgc gcagcgccga gcgtcggtgg tcgccgcgca gacgccagcc
     5701 caacaccttc gcgagcacgc gcgcgatcat gacgagctcg ggcaagccgt ccttcggaac
     5761 cgcggagggg ccacccttcg cgctgttctt gaatccgcgg ccgcggatcg gaccgagcgc
     5821 cgccgacggt gcatcgatga atgctttgat cacgccgatg aacggcatgg cgctcgtggg
     5881 catcgtgttg ccgatcggcg tgcggcagca gctcgcatac caacggatca ttcccttggg
     5941 ggagagcctc atcgcagcga ccttgtcgac gccggccgcg aaccggaccg tcgcctgcga
     6001 catctgcgta atctccgtgc cgccgtgcgc gtcgaggacc tcgtcggcac ggcccagaaa
     6061 atgtgcgaac gcctggcagt cgtcgcagta gcaaaaacag tgattgatcg agctcggcgt
     6121 cacgccgcgc gcgatgccgc gcaccgtacc gcagcggcaa cgcagcgcga gatcggtgcc
     6181 ttgctccacg cgtatgcgtc agtcgcgtcg atcgacgctc agtgcgagcc cgctggctgc
     6241 tcgagcgcgg ccaaatcgcc ggcggcgtac acgatctcgc cgccgagcag cgtcaggacc
     6301 gactcgatgt tgccgacctc gtcgagcggc acggtgaggt agtcctgcga cagcacggca
     6361 agatcggcaa gctttccgac ttcgagcgag ccgcgcacgt cctcgtcgaa cgagaaccaa
     6421 gcgctgccga gcgtgtagaa cttcagcgcc tgcagccgcg acggcgtttc ctcggggccg
     6481 cgaatcgccg tgccggcgac cgtcttgccg tcgaggaacc actgcagcga cgtgaacggg
     6541 ttgtacgacg c
//
LOCUS       DATLEE010000408         5531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9397811, whole genome shotgun sequence.
ACCESSION   DATLEE010000408 DATLEE010000000
VERSION     DATLEE010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9397811"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VM692_08725"
     CDS             complement(<1..777)
                     /locus_tag="VM692_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HUQ52293.1"
                     /translation="MTTRLEPTDVVVIGLGGAGGVAVEPLAAAGMKIVGLEAGSRLGP
                     RDFAPDELRNNFRGWPHAAQKCNREIPVHRPNANAPDSPRPPVHVMMNGVGGTTLHYW
                     AQSWRLNPWDFKVVSETTRRYGAGRIPRGSTVEDWPFGLEELEPYYDKVELALGVSGQ
                     AGNVKGVIDPRGNIYEGPRERPFPMPPLRGTEFTDLMGRTATRLGWHPFPGPAAVNSE
                     VYQNRSACLYHGFCNRGGCHVSAKNSTAVATIPRAEATGNL"
     gene            complement(767..1408)
                     /locus_tag="VM692_08730"
     CDS             complement(767..1408)
                     /locus_tag="VM692_08730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HUQ52294.1"
                     /translation="MKRRLTRRDMLRRSAALGTVAAVPANVLAQAAQAAPYVHLTAAE
                     AATLEAVVARLIPADRSGPGALEAGAARYIDNALGDALAGQRPAYSAGLAALDAYARA
                     QAGGSFASLAPARQDELLTELEQNRARGFTPSSSAFFELVLGHTLEGTFGDPHYGGNQ
                     GYIGWELIGYPGLRLAVTAEQQQMNAALEPIRSSAYDHAQFDAHTTAGERDDD"
     gene            complement(1413..2078)
                     /locus_tag="VM692_08735"
     CDS             complement(1413..2078)
                     /locus_tag="VM692_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUQ52295.1"
                     /translation="MRSRLVVTWLCSLGLAGLAAAQTAAPAGDLDPKLVGTRFAPLQW
                     SQMSEAQKTMMRHVLDGPRTAANGPFNVMARSPVMGDLAQELGAQVRFNSTLPPALRE
                     MAILMAARHWTGQYEWQAHKALALEAGLDPKVVAAIATGAEPASMQAKEAALHRFCSE
                     LLATKHVGNDTFAAAKAAFGEQGMTEIMYTLGYYSMVSMMLNVDEYPLPAGQTPELKP
                     LAN"
     gene            2212..3228
                     /gene="pyrB"
                     /locus_tag="VM692_08740"
     CDS             2212..3228
                     /gene="pyrB"
                     /locus_tag="VM692_08740"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HUQ52296.1"
                     /translation="MSETLVLPTRASLFHVIDPRVFERRLLDELCSLATRVRLLAKTD
                     SGSRLLRDLLPTRRAMLYFTQPSTRTFLSFNNACHILGMRTSEIRDPSTSSEVKGESF
                     EDSIRTFSSYVDVIVMRTKESGRAAQAAKLMNGIPRPVPVINAGSGSDQHPTQALLDI
                     YTLQRSFEEQGGIDGKVIGMMGDLKRGRTVRSLCYLMKSYQKVRLVFIAPEQFAMEPD
                     VKAYLDQHGIPYSETTKLADVLPTLDALYVTRMQTEWDTAGESRAVDLTRFSIGPQEL
                     SRLKRDAIIMHPLPRGAEIAPSVDQDPRAMYWRQERNGMWMRVAVLIKIFGADVSLAE
                     IDPQ"
     gene            3243..4598
                     /locus_tag="VM692_08745"
     CDS             3243..4598
                     /locus_tag="VM692_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52297.1"
                     /translation="MLAETLIRWRKGRELKRVAQLTRAELEAAKLAKFRKLVAHAQQR
                     SPYYRRIIAERGIDVTACTPEQFPVLTKSLLMQNFDEISTAPGVTKKAIAEFLTRSHD
                     PTELFLDRYRVIHTSGSSGEVGYFVYSKRDWANGTAMRPQGGRPKRKGGGKGKFRLAY
                     FAAVDGHYAGVTMIGAFQSGLAKLFVDCKMFEVNDPLPETLAKLNAFQPEVLIGYTTA
                     LKILADAQRRGALQLDRVIFITTAGEATTPADITLLSETFGCGVVNSYGCSEHLGMGA
                     SPPNGDKIVLADHDLVFEFMPDHTLVTNLFNYTLPLIRYRMADVLRPVPSGEHAPYLV
                     IESLVGRNELQPVFKNRDGVEDFISPHTINEVFVAGVTRFQLQLTGADAFTFMICLDP
                     KLGAAERATAIEGVKARLREILARKRMDNVRFDVVATDDLPVNPRTRKFQLIVDRRDA
                     A"
     gene            complement(4639..5238)
                     /locus_tag="VM692_08750"
     CDS             complement(4639..5238)
                     /locus_tag="VM692_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HUQ52298.1"
                     /translation="MLLKRIGATAALLLATFTSATALAQGGGGGGAGGGGGGPPRLPP
                     LLMQSTAFEDGGVVPQKYAGRDGVQPGFTFSNAPDTTVSYAIIFHDLDVSLQGSTNDV
                     LHWIAWNIPASAKGIPEGKLPDGSVTGKNIAGQNSYFGPGAPPGPRYHHYVFELYALS
                     ANLDLPNTAGRPELLAAMNGKIVAKAAYVGRFRGTPPPQ"
     gene            complement(5298..>5531)
                     /locus_tag="VM692_08755"
     CDS             complement(5298..>5531)
                     /locus_tag="VM692_08755"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52299.1"
                     /translation="NPHIWLQVVVEQNGAPTEWSLEGGSPNTLSRQGWRSTTFKPGDV
                     IAVKLNPMRDGAPGGRFIGARFDADGHTIGRWE"
BASE COUNT          906 a         1880 c         1820 g          925 t
ORIGIN      
        1 caaattgccc gtcgcctcgg cgcgcggaat cgtcgccacg gccgtcgagt tcttggccga
       61 gacgtgacag ccgccgcgat tgcagaagcc gtgatagagg cacgcgctgc gattctgata
      121 gacctcggag ttgacggcgg ccggccccgg aaacggatgc cagccgagcc gcgtcgccgt
      181 gcggcccatg aggtccgtga actccgtacc gcgcaacggc ggcatcggga agggccgctc
      241 gcgcgggcct tcgtagatat tcccgcgcgg atcgatgacg cctttgacgt tgccggcttg
      301 tcccgacacg ccgagcgcga gctcgacctt gtcgtagtac ggctcgagct cctcgaggcc
      361 gaacggccaa tcttcgacgg tcgagccgcg cggaattcgg cccgcaccgt agcggcgagt
      421 ggtctcgctg acaaccttga agtcccaggg attcaggcgc cagctctgcg cccagtagtg
      481 cagcgtcgtg ccgccgacgc cgttcatcat gacgtggacc ggcggccgcg gactgtcggg
      541 tgcgttcgcg ttcggccggt gcacggggat ctcgcgattg cacttctgcg cggcgtgcgg
      601 ccagccgcga aagttgttgc gcagctcgtc gggcgcgaag tcgcgcgggc cgagccgcga
      661 gccggcttcg aggccgacga tcttcatgcc ggccgccgcc agcggctcga cggcgacgcc
      721 gcccgcgccg cccaggccga tcacgacgac gtcggtcggc tccagcctag tcgtcatcgc
      781 gctcgccggc cgtcgtgtgc gcgtcgaatt gcgcgtggtc gtaggccgag cttcgaatcg
      841 gctcgagcgc cgcgttcatt tgctgctgct ccgccgtcac ggccagacgc aagcccgggt
      901 agccgatgag ctcccaaccg atgtagccct ggttgccgcc gtagtgcggg tcgccgaacg
      961 tgccttcgag cgtgtgaccc agcacgagct cgaagaaggc actggacgag ggcgtgaaac
     1021 cccgcgcgcg gttctgctcg agctccgtca ggagctcgtc ctgccgcgcc ggcgcaagcg
     1081 aggcgaacga gccgccggcc tgcgcgcgcg cgtaggcgtc gagcgccgcg agtcccgctg
     1141 agtacgcggg ccgctgcccg gcgagcgcat cgccgagcgc attgtcgatg taacgcgcgg
     1201 cgccggcttc gagcgcgccg ggcccgctgc gatcggcggg aatcagtcgc gcgacgacgg
     1261 cttcgagcgt cgcggcctcg gccgcagtga ggtggacgta gggtgcggcc tgcgccgctt
     1321 gcgcgagcac gttcgccggc actgccgcga cagttccgag cgccgcgctg cggcgcagca
     1381 tgtcgcgacg agtcaggcgc cgcttcatgc gctcagttcg cgagcggctt cagctcgggc
     1441 gtctggccgg cgggcagcgg gtactcgtcg acgttcagca tcatcgaaac catgctgtag
     1501 tagccgagcg tgtacatgat ctcggtcatg ccctgctcac cgaaggcggc cttggcggct
     1561 gcgaacgtgt cgttgccgac gtgcttcgtc gccagcagct ccgagcagaa gcggtggagc
     1621 gcggcttcct tggcctgcat cgaggctggc tcagcgccgg ttgcgatggc cgccacgacc
     1681 ttgggatcga gccccgcctc caatgcgagc gccttgtgcg cttgccattc gtattggccg
     1741 gtccagtgcc gagcggccat gaggatcgcc atctcgcgca gcgccggcgg tagcgttgaa
     1801 ttgaaccgca cctgcgcacc gagctcttgc gcgaggtcgc ccatgacggg agagcgcgcc
     1861 atgacgttga acgggccgtt cgccgccgtg cgcgggccgt cgagcacgtg gcgcatcatg
     1921 gttttctgcg cttccgacat ctgcgaccac tgcagtggcg cgaaccgagt cccgacgagc
     1981 ttagggtcga gatcgccggc cggcgccgct gtttgtgccg cggccaagcc tgcgagaccc
     2041 agggagcaga gccacgtcac gacgagtcga ctacgcatag gctgactccg agccgattgg
     2101 ggttcaattc gagctgtcga gacgatacac cacgcccgcg ttgcggcgta caatttccgt
     2161 cgcgcgccgc gccatcccac ttcagaggct attgcaatcg ctaccgaacc cttgagcgag
     2221 acgctggtcc tgcctacgcg ggcctcccta ttccacgtca tcgatccgcg cgtgttcgag
     2281 cggcggctgc tcgacgagct ctgctcgttg gcgacacgag tgcggttgct cgctaaaacg
     2341 gattccggct cgcgcctgct gcgcgacctg ctgccgacgc gccgcgcgat gctgtacttc
     2401 acgcagccct cgactcgaac gttcctgtcg ttcaacaacg cgtgccacat cttgggcatg
     2461 cgcacgagcg aaatccgcga cccgtcgacg tcgtcggagg tcaaaggcga aagcttcgaa
     2521 gactcgattc gcaccttcag ctcgtacgtc gacgtcatcg tcatgcgcac gaaggagtcg
     2581 gggcgcgcag cgcaggccgc caagctcatg aacggcattc cgcggcccgt gcccgtcatc
     2641 aacgccggca gcggcagcga ccagcacccg acgcaagcct tgctcgatat ctacacgctg
     2701 cagcgcagct tcgaggagca aggcggcatc gacggtaagg tcatcggcat gatgggcgac
     2761 ctgaagcgcg gccgcaccgt ccgctcgctc tgctacctca tgaagagcta ccagaaggtg
     2821 cggctcgtgt tcatcgcgcc cgagcagttc gcaatggagc ccgacgtcaa ggcctacctc
     2881 gatcagcacg gcattccata cagcgaaacg acgaagctcg ccgacgtgtt gccgacgctc
     2941 gacgcgctgt acgtcacgcg catgcaaacc gagtgggata cggccggcga gtcgcgcgcc
     3001 gtggatctca cgcgcttcag catcggcccg caggagctct cgcggctcaa gcgcgatgcg
     3061 atcatcatgc atccgctgcc gcgcggcgcc gagatcgcgc cgtcagtcga ccaggacccg
     3121 cgcgcgatgt attggcggca agagcgcaac ggcatgtgga tgcgcgtcgc cgtgctgatc
     3181 aagatcttcg gcgccgacgt gtcactagcg gaaatcgacc cgcaataacc ctgcaggctg
     3241 acatgctcgc cgagacgctc atccgttggc gtaaagggcg cgagctcaag cgcgtggcgc
     3301 agctcacgcg cgccgagctc gaggcggcca agctcgcgaa gttccgcaag ctcgtcgccc
     3361 acgcgcagca gcgctcgccg tattaccggc gcatcatcgc cgagcgcggc atcgacgtga
     3421 ccgcctgcac gcccgagcag tttcccgtgc tcacgaaatc gctgctgatg cagaacttcg
     3481 acgagatctc gacggcgccc ggcgtcacga aaaaggccat cgccgagttc ctgacgcgct
     3541 cgcacgatcc gacggagctg tttctcgacc gttaccgcgt catccacaca tcgggctcgt
     3601 cgggcgaggt cgggtacttc gtctactcga agcgcgactg ggctaacggc acggcgatgc
     3661 ggccgcaagg cgggcgaccg aagcgcaaag gcggcggcaa gggcaagttt cggctcgcgt
     3721 acttcgcggc cgtcgacggt cactacgcgg gcgtgacgat gatcggcgcg ttccaaagcg
     3781 ggctcgcaaa gctgttcgtc gattgcaaga tgttcgaggt caacgacccg ttgccggaaa
     3841 cgctcgcaaa gctcaacgcg tttcagcccg aggttctgat cggctacacg acggcgctga
     3901 aaattctcgc cgacgcgcag cgtcgcggcg ctctgcagct cgatcgagtc atcttcatca
     3961 cgacggccgg cgaagcgacg acaccggccg acattacgct gctgtcggaa acttttggct
     4021 gcggtgtcgt gaacagctac ggctgcagcg agcacttggg catgggcgcg tcgccgccga
     4081 acggcgacaa gatcgtgctc gccgatcacg acctcgtgtt cgagttcatg cccgatcaca
     4141 cgctcgttac gaatctgttc aattacactc tgccgctgat ccgttatcgc atggccgacg
     4201 tgctgcgccc cgtgccctcg ggtgagcacg cgccgtacct cgtcatcgag agcctcgtcg
     4261 gccgcaacga gctgcaaccg gtattcaaga atcgcgacgg cgtcgaggac ttcatcagcc
     4321 cgcacacgat caacgaggtc ttcgtcgccg gcgtcacgcg ctttcagctc cagctcacgg
     4381 gcgccgacgc gttcacgttc atgatctgcc tcgacccgaa gctcggcgca gccgaacgcg
     4441 cgacggcgat cgaaggcgtc aaggcgcgcc tgcgcgagat cctcgcgcgc aagcgcatgg
     4501 acaacgtgcg gttcgacgtg gtggcgaccg acgacttgcc cgtaaatccg cgtacgcgga
     4561 aatttcagct catcgtcgat cgccgcgacg ccgcttgaga agcggcgggc ggccgttcgg
     4621 ccgcccgccg tcgaacgcct attgcggcgg cggcgtgccg cggaagcggc cgacgtacgc
     4681 ggccttcgcg acgatcttgc cgttcatcgc cgcgagcagc tcggggcggc ccgcggtgtt
     4741 cggcagatcg aggttcgcgc tgagcgcgta gagctcgaac acgtagtggt gatagcgagg
     4801 ccccggcggc gcgcccggcc cgaagtacga gttctggccc gcgatgttct tgcccgtcac
     4861 ggagccgtcc ggcagcttgc cttcgggaat tcccttggcc gaggccggaa tgttccaggc
     4921 gatccagtgc agcacgtcgt tcgtgctgcc ctgcagagag acgtcgaggt cgtggaagat
     4981 gatcgcgtag ctgaccgtcg tgtccggcgc attcgagaac gtgaagcccg gctgtacgcc
     5041 gtcgcgcccc gcgtacttct gcggcacgac gccgccgtcc tcgaacgccg tgctctgcat
     5101 caacagcggc ggcaaacgcg gcggaccgcc gccaccaccg ccagctccgc caccgccgcc
     5161 gccctgggcg agcgccgtcg cgctcgtgaa cgttgccagc agcagcgcgg ccgtcgcgcc
     5221 aatccttttc aatagcatgg tcatggtcct cgtgggttgc gtgaaaaatg agtggaatac
     5281 gcgagcgttg cgaatcgcta ttcccagcga ccgatggtgt ggccgtccgc gtcgaagcgc
     5341 gcaccgatga accggccgcc cggcgcgccg tcgcgcatcg gatttaactt cacggcgatc
     5401 acgtcgccgg gcttgaacgt cgtgctacgc cagccttgac gcgacagcgt gttcgggctg
     5461 cccccttcga ggctccattc ggtcggcgcg ccgttctgct cgacgacgac ctgcagccag
     5521 atgtgcggat t
//
LOCUS       DATLEE010000409         4198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4163799, whole genome shotgun sequence.
ACCESSION   DATLEE010000409 DATLEE010000000
VERSION     DATLEE010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4163799"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..241)
                     /locus_tag="VM692_08760"
     tRNA            complement(166..241)
                     /locus_tag="VM692_08760"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Gly,seq:gcc)
     gene            complement(298..876)
                     /gene="pgsA"
                     /locus_tag="VM692_08765"
     CDS             complement(298..876)
                     /gene="pgsA"
                     /locus_tag="VM692_08765"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HUQ52300.1"
                     /translation="MKFNLPNALTWFRVVAIPLVVAVFFGTDLFGWEETWARPAAGLL
                     FGLAGITDYFDGYLARRLGLTSNFGAFLDPVADKLIVSTALVLLVQGDPQIILALVAA
                     VIIGREITVSALREWMSQIGARAHVAVSIFGKWKTTLQIIGISFMLYREPLLTLPIYR
                     IGEWLIYVAAALTLWSMIDYLRAAWPAMRAHD"
     gene            complement(889..2745)
                     /gene="uvrC"
                     /locus_tag="VM692_08770"
     CDS             complement(889..2745)
                     /gene="uvrC"
                     /locus_tag="VM692_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HUQ52301.1"
                     /translation="MHEEPAVAPAEEPAVAFDPANVLKHLTHRPGVYRMLDDAGTVIY
                     VGKARDLKRRVTSYFQGSRAHDAKTLAMVRTIAGIEVTVTRTEVEALMLEYNLIKEHR
                     PRFNVMLRDDKSYPYVLLETEHRFPRLSFYRGPRTSKGKLFGPYPNAGSVRESLNQLH
                     KLFQLRQCEDSYFENRSRPCMQHQIERCTAPCVGLVSEEDYARDLEHAVLFLEGQSDV
                     VTTRLAQRMEQASSALQFERAAQYRDQLARLKNVQSQQLMARASGEFDAVGMAEDHGL
                     VCVAVMFFRGGRSLGTRSFFPKLVKGADDDEIMRAFLLQYYGGREAPREILVNRPVPE
                     AATLAELLSSQSGHKVAIKSSVRGDRARFVEMVVTNARHSAELKYQASASYERQLEDL
                     CEVLGLDEPPTRLECFDVSHTAGEATVASCVVFGPEGPLKSDYRRFNVENVAPGDDYG
                     AMTQVLSRRYARVQKGEVPMPDVVFIDGGPGQLARAIAVMQELELPASRLVGVAKGQD
                     RKPGRESLYLPDSTDPLRLPPSSPALHLIQQLRDEAHRFAITGHRARRQKARTRSPLE
                     DIPGLGPKKRRELLKQFGGLQALTRAGIDDLQRVRGISRQLAEAIYERFHAD"
     gene            complement(2762..4120)
                     /locus_tag="VM692_08775"
     CDS             complement(2762..4120)
                     /locus_tag="VM692_08775"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HUQ52302.1"
                     /translation="MTQAQVPTDWHPASWQSRHAAQQPVYRDRAHLESVLAQLSRLPP
                     IVTTWEIENLKSQLAKAQRGEAFLLQGGDCAESFDECTSDNVVQKLKILLQMSLVMLV
                     GLKKPIIRVGRMAGQYAKPRSADYETKDGISLPSYRGDTINRPGFTAAEREPDPTLML
                     RGYERAALTLNFVRSLIDGGFADLHHPENWDLTFVGESALAADYHKLVQSVSDSLEFF
                     EAIVGEPMHETHRVEFFTSHEGLQLPYEQAQTRFLGHRQHWYDLATHYPWIGMRTADL
                     NGAHVEYFRGIANPIAVKIGTSVTDEHLQQLIHVLNPHNEPGRLTLITRFGAKNIEAH
                     LPRVIRAVRSTGSGVLWVCDPMHGNTETTAEGYKTRRFDNIRAELRSAFRIHQEQGSY
                     LGGVHLELTGENVTECIGGARGLKESDLARAYKSQVDPRLNYEQAMEIAMLIGGRLRT
                     HD"
BASE COUNT          725 a         1359 c         1374 g          740 t
ORIGIN      
        1 ccaaacactg tcggtcggcg tgctcggcca ggtccgtctg cagggtcggt cgtactcgtt
       61 atgggcaatg cccgaggtcc gcaactgtgg cgcaaaaaaa aacgcccaaa cctatttaga
      121 tttgggcgtt tagcgcactg acccggatgg gtctgcaaga gtaagtggag cgggaaacga
      181 gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg
      241 cgtagagggc gccattctac aggcgggccg acgcgggcgg caacgggcta gttcacgtca
      301 gtcgtgcgcc cgcatggcgg gccaggcggc gcgcaaataa tcgatcatcg accataacgt
      361 cagggccgcg gcgacgtaga tgagccactc gccgatccga tagatcggca acgtcagcag
      421 cggctcgcgg tagagcatga agctgatgcc gatgatctgc agcgtcgtct tccatttgcc
      481 gaagatgctg acggcgacgt gcgcccgggc accgatctgg ctcatccatt cgcgcagcgc
      541 ggacaccgtg atctcgcggc ctatgatgac ggcagccacg agcgcgagga tgatctgcgg
      601 gtccccttgc acgagcagca cgagcgcagt ggacacgatc agcttgtcgg cgacgggatc
      661 gaggaacgcg ccgaagttcg aggttaggcc caagcgccgc gcaagataac cgtcgaagta
      721 atcggtgatg cccgcgagcc cgaacaacaa gcccgccgcg ggccgcgccc aggtctcctc
      781 ccaaccgaat agatcggtgc cgaagaacac ggcgacgacg agcggaatcg ccacgactcg
      841 aaaccaggtc agcgcattcg gcaggttgaa cttcattagt gtgagccgtc agtcggcatg
      901 gaatcgctcg tagatcgcct cggccagctg acgcgagatc cctctaactc gttgcaagtc
      961 gtcgataccg gcacgcgtta gagcttggag gccgccgaac tgcttcagca gctcgcgccg
     1021 cttcttcggc cccaaccccg gaatgtcctc gagcggcgag cgggtgcggg ctttctgccg
     1081 ccgcgcgcga tgacccgtga tcgcgaaccg atgcgcctcg tcgcgcagct gctggatcaa
     1141 gtgtaatgcc ggagagctcg gcggcaaacg cagcgggtcc gtgctatcgg gcagatacaa
     1201 gctttcgcgt cccggcttgc ggtcttgtcc tttggcgacg ccgacgaggc gcgaggccgg
     1261 cagctcgagc tcttgcatga cggcgatcgc gcgcgcgagc tgacccggtc cgccgtcgat
     1321 gaacacgacg tccggcatcg gcacctcgcc cttctgcacg cgcgcatagc gccgcgacaa
     1381 aacttgcgtc atggcgccgt agtcgtcgcc gggcgcgacg ttctcgacgt tgaagcggcg
     1441 atagtcggac ttcagcgggc cctcggggcc gaacacgacg cacgacgcga ccgtggcctc
     1501 gccggccgtg tggctcacgt cgaagcattc gagccgcgtc ggcggctcgt cgagccccaa
     1561 gacctcgcag agatcctcga gctgccgctc gtacgacgcg ctcgcttggt acttgagctc
     1621 cgcgctgtgg cgcgcgttcg tgacgaccat ctcgacgaag cgcgcgcggt cgccgcggac
     1681 gctcgatttg atcgcgacct tgtggcccga ctgggacgac agcagctcgg ccagcgtggc
     1741 ggcttcgggc acgggccggt tcacgaggat ttcgcgcggc gcctcgcggc cgccgtagta
     1801 ctgcagcaag aatgcccgca tgatctcgtc gtcgtcggcg cctttgacga gcttcggaaa
     1861 gaagctgcgc gtgccgagcg agcggccacc gcggaagaac atcacggcga cgcacaccaa
     1921 accgtgatcc tcggccatgc cgacggcatc gaactcgccg ctcgcgcgcg ccatgagctg
     1981 ctgcgattgc acgttcttca ggcgcgcgag ctggtcgcga tactgcgcgg cgcgctcgaa
     2041 ttgcagcgcg ctcgaagcct gctccatacg ctgcgcgagc cgcgtcgtga cgacgtcgct
     2101 ctggccttcg aggaacagca cggcgtgctc gaggtcgcgc gcgtagtctt cctcgctgac
     2161 gaggccgacg cacggcgccg tgcagcgctc gatctggtgc tgcatgcacg gccggctgcg
     2221 attctcgaag tagctgtcct cgcattggcg cagctggaac agcttgtgga gctgattcag
     2281 cgactcgcga acggagccag cgttcggata cgggccgaac agcttgcctt tcgacgtgcg
     2341 cgggccgcgg tagaacgaca agcgcgggaa gcgatgctcg gtctcgagca agacgtacgg
     2401 atagctcttg tcgtcgcgca acatgacgtt gaagcgcggc cgatgctcct tgatgaggtt
     2461 gtactcgagc atcagcgctt cgacttccgt gcgcgtgacg gtgacctcga tgccggcgat
     2521 cgtgcgtacc atcgcgagcg ttttcgcgtc gtgggcgcgg ctgccttgga aatagctcgt
     2581 gacgcggcgc ttcaagtcgc gcgcttttcc gacgtagatg accgtgcccg catcgtcgag
     2641 catgcggtag acgccggggc ggtgcgtcaa gtgcttgagg acgttcgcgg gatcgaaggc
     2701 gacggcgggc tcttccgccg gagcgacagc gggctcttcg tgcatcacga cggcatcgac
     2761 cctagtcgtg cgtgcgcagg cgcccgccga tcaacatcgc gatctccatc gcctgctcgt
     2821 agttgagccg cgggtcgact tgcgacttgt aggcgcgcgc gagatcggat tctttgaggc
     2881 cgcgcgcgcc gccaatgcac tcggtgacgt tctcgccggt gagctcgagg tgcacgccgc
     2941 ccaagtagct gccctgctct tgatggattc gaaacgccga acgcagctcg gcccgaatgt
     3001 tgtcgaaccg gcgcgtcttg tagccctcgg ccgtcgtctc ggtattgccg tgcatcggat
     3061 cgcagaccca caggaccccc gagcccgtcg agcgcacggc gcggatcacg cgcggcaaat
     3121 gcgcctcgat gttcttcgcg ccgaagcgcg tgatcaacgt cagccggccc ggctcgttgt
     3181 gcgggttcag tacgtgaatc agctgctgca agtgctcgtc cgtgacgctc gtgccgatct
     3241 tcacggcgat gggattcgcg atgccgcgga agtattcgac gtgcgcgccg ttcaaatccg
     3301 ccgtccgcat gccgatccac ggatagtgcg tcgcaaggtc gtaccagtgc tgccgatgac
     3361 ccaggaaccg cgtctgcgcc tgctcgtacg gcagctgcaa cccttcgtgc gacgtgaaga
     3421 actcgacgcg atgtgtctcg tgcatcggct cgccgacgat cgcctcgaag aactcgagcg
     3481 aatccgatac cgactgcacg agcttgtgat agtccgcagc gagcgccgac tcgccgacga
     3541 acgtgagatc ccagttctcg ggatggtgca gatccgcgaa cccgccgtcg atcaacgacc
     3601 gcacgaaatt caacgtcagc gccgcacgct cgtagccgcg caacatcaac gtcgggtcgg
     3661 gctcgcgctc ggccgccgta aagccgggcc ggttgatcgt gtcgccgcga tagcttggca
     3721 gcgagatgcc gtccttcgtc tcgtagtcgg cgctgcgcgg cttcgcgtac tgccccgcca
     3781 tgcggccgac acggatgatc ggcttcttga ggcccacgag catcacgagc gacatctgca
     3841 gcaggatctt cagtttctgc acgacgttgt ccgacgtgca ctcgtcgaag ctctcggcgc
     3901 aatcgccgcc ttgcagcagg aacgcttcgc cgcgctgggc tttggcgagc tgcgatttca
     3961 ggttttcgat ttcccaggtc gtgacgatcg gcggcaggcg gctcagctgc gcgagcacgc
     4021 tctccaagtg ggcgcggtcg cggtaaacgg gctgctgcgc ggcgtgtcgg ctctgccaac
     4081 tcgccggatg ccaatcggtt gggacttggg cttgcgtcat gacggcgcac tataccgccg
     4141 ggccgcgcgc gaccaaagga aaccggccgc aacggccgga aacggcgctt cgttgcgg
//
LOCUS       DATLEE010000410         7253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5676789, whole genome shotgun sequence.
ACCESSION   DATLEE010000410 DATLEE010000000
VERSION     DATLEE010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5676789"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VM692_08780"
     CDS             <1..184
                     /locus_tag="VM692_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52303.1"
                     /translation="FEADLFDTHEAPKAAQLPAMRTLATQVERMRLDVATLAPVHGAP
                     VPWREFTSALRALEVR"
     gene            complement(181..1167)
                     /locus_tag="VM692_08785"
     CDS             complement(181..1167)
                     /locus_tag="VM692_08785"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HUQ52304.1"
                     /translation="MSTAGECQNCHAALAGPFCGVCGQKGDVRIPTLGHLLADALGDL
                     LNFDSRIWRSLVVLTLSPGRLTRLYLEGKRVRYAPPFRMYIVTSVVFFLLFSFARFGA
                     LSPTGPEVLDSIDDSVNATVNGALSDAGVAPPAPAEPDAAAPTTAAPGGPQTFNVSVD
                     DNGRWECQLDRNLEPRFRARLEAACRQIERDNGASFINAFARNFPVMMLVFIPIMAAI
                     MKVLYLFARRKYVEHLLFFLHVHTFFFLTGVVVILVSRAARLVPGLEWPALIVEIAAS
                     IYFLLYVYRAMRRVYVQGHLLTSVKYVVLGGSYFVAFLMTMLGLIVYTALTL"
     gene            1258..1554
                     /locus_tag="VM692_08790"
     CDS             1258..1554
                     /locus_tag="VM692_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52305.1"
                     /translation="MTFLVFCVFDLKSATREDYLYAYMDLATLGLRQTVKSDNGPSFK
                     LPAAAVMGTFDGRSVDDVRGNISRKVQGIFRARGLNGTFFLVASHDWACAGEST"
     gene            1627..2520
                     /locus_tag="VM692_08795"
     CDS             1627..2520
                     /locus_tag="VM692_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ52306.1"
                     /translation="MRHLILVATLAALAAFSSGAQQAQAQRGQNEAATPADPGAGFGP
                     QQLTTFKVRDNFYMIRSGQSGNCSVLIADDGVVLIDNKFEMDHAGIMTKLRELTDKPV
                     LYVINTHMHADHSGGNATKQALGAKVIAHENARFQMAMTQTTGLPNLTLEDHMRLYHG
                     EFVLDLYYLGRGHTDGDIVVHLPEQKMIFMGDLFATWDPYVTLIHYAAGGSLREWTRT
                     LDRALALPFDTVIPGHGGDTDRAHLETYRAQLVRMQDMVREMNRTKKTRADIQKMLEV
                     EFGWSGFVTNLGLDGVIGEMQ"
     gene            2544..3518
                     /locus_tag="VM692_08800"
     CDS             2544..3518
                     /locus_tag="VM692_08800"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HUQ52307.1"
                     /translation="MIASDFVPPPRRPFAPWLRYASYVPLYGLALPIVKTLEAFGAWP
                     QVMSRRAHRDASPFADYRPSGGDVFVCAYFKSGTTWTLQMATQIAFRGQAEFANIHHV
                     VPWPDVPMPPMQRMMVPLADPSPLARSPTGRRVIKTHLTRAEIPFVAEARYIAVVRNL
                     KDVCVSGYHFLKSMAFGPLMPSVNRWVDYLLSPDFRPPWAEHLASYWAVRHEPNVLFL
                     TYEHMKQDHVGAVRKMAEFMGIELTLGELQSVVEQSTFAAMKAGGLRFEPGRVVPWGQ
                     ERSMLRRGESGRSSELLTPEQQARIDDWCRAELARLQCDFPYDEAFGR"
     gene            complement(3540..4559)
                     /locus_tag="VM692_08805"
     CDS             complement(3540..4559)
                     /locus_tag="VM692_08805"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52308.1"
                     /translation="MRHAHVRAAHVAATAAAVLLARGALAHHSPAGYDMQSQHTVEGT
                     IAEYEWGNPHVYLSVRENGSDKVWVVEAFPSTAMKQYGWSKQTFALGDRVVVTGHPGR
                     NPARTVLFLQAVRRADAAAMLYDAKGAVAAPPSAPSQTFRATSLAGTWSSSVGPAFGN
                     FFGPGVAQLGTPQGAAAVAEFRDAANPGLECVPFTIPVYMILPGFRSIDVRADAVVIR
                     GEDAAVDRTVHLDAATHEGAAPAVQGHSIGRWDNGVLVVDTANFTPHRIGNGAGLPSG
                     TRKHLVERFALNAQGGLTYSFELEDPEFLKQRVTGTADWLYRPDVEFTATPCSRDNAQ
                     RFLAE"
     gene            complement(4567..5775)
                     /locus_tag="VM692_08810"
     CDS             complement(4567..5775)
                     /locus_tag="VM692_08810"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52309.1"
                     /translation="MKQQFQTLGAGLLIASLAAAPVALAHHGFGNFAMDEDITVSGVV
                     TKIDFVNPHSWVHFTQTNPDGTVTQRRCELRSATTLRRSGWTPEMFAVGTKITIQGSP
                     DRNEKDACYTSTLTFADGTVLDRYGQRIAAQAPTTRAARTADGKPNLAGDWAVEQLVM
                     TDAAGRDGTLVPLSTVGNFARGAVPEGQREIPGARGTPEAQTPRTAPAGAGGGGGAFG
                     RPAVALTPAGEAAQKALPQLSRAERACAPGSIVSDWGGEPVNRITQTPNAITIQYGRT
                     GMSRIVNVGMAAHPANVAPSRAGHSIGKWENDVLVVDTVGFLPGTLVGTTPHSAALHV
                     VERFSLDPAKNTLKRDVTAEDAQFFTAPYTSSTTMLVSTVPYEAEVCQDLTPVVPPAP
                     AAAPAAAQRQ"
     gene            5937..6929
                     /locus_tag="VM692_08815"
     CDS             5937..6929
                     /locus_tag="VM692_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52310.1"
                     /translation="MDRRIRYLAAAAALAAPLLIAEMLAAQPGEAAWTAARTQAGQPD
                     LQGHWTNDTYTPLERPAELGDKQVFTPEEAAAFLRSRVDDLNGQAADDVHYDDAIWQA
                     ENYDKVAHLHTSLVLEPHNGRLPKLTAHGEDRLPRQRAMQRATSADSAQSRSLAERCI
                     SWGNVGPPMVPPTYNANLQILQSRDIVVIRHEMMHDVRTVYLEGRAHPPAELQWLAGH
                     SIGRFEGDTLVVDTTNFTPLTNFRGSPQNTRQDIFASEHLHVVERFTRTSDDNIHYEF
                     TVDDPETWVEPWSGEMEIRRTEGPIYEYACHEGNLGLANILRAARVAERQATRQ"
     gene            complement(7027..>7253)
                     /locus_tag="VM692_08820"
     CDS             complement(7027..>7253)
                     /locus_tag="VM692_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ52311.1"
                     /translation="GTYHVLTNDQIKLDGDHASATTKWIYITPGTDNAPKLVYLGHYD
                     DQFVRENGEWKFLRREAPADIPAGPPSGSR"
BASE COUNT         1280 a         2403 c         2383 g         1187 t
ORIGIN      
        1 gttcgaggcc gatttgttcg acacgcacga ggcgccgaaa gcagcccagc tgccggcgat
       61 gcgcacgctc gcgacgcagg tcgagcgcat gcggctcgac gtcgcgacgt tggcgcccgt
      121 gcacggcgcg cccgtgccgt ggcgcgaatt cacgagcgcg ttgcgcgcgc ttgaagtccg
      181 ctagagcgtc agcgccgtgt agacgatcag gccgagcatc gtcatcagga aggcgacgaa
      241 atagctcccg ccgagcacga cgtacttgac cgacgtcagc aagtgtcctt gcacgtacac
      301 gcgccgcatc gcgcggtata catagagcag aaaataaatc gaagctgcga tttcgacgat
      361 gagcgccggc cactcgagcc ccggcaccag ccgcgctgcc ctactaacga ggatcacgac
      421 cactccggtc aagaagaaga acgtatgcac gtgcaaaaag aacagcagat gctcgacgta
      481 cttgcggcgt gcgaacaggt agaggacctt catgatcgcc gccatgatcg ggatgaacac
      541 gagcatcatc accggaaagt tgcgggcgaa cgcattaatg aacgaagcgc cgttgtcgcg
      601 ctctatctgg cggcacgccg cctcgagccg cgctcgaaac ctcggctcga gattgcggtc
      661 gagctgacat tcccagcggc cattgtcgtc gaccgatacg ttgaaggttt gcggtccgcc
      721 cggtgcagcg gtagtgggcg ccgccgcgtc gggctcggcc ggcgcgggtg gcgcgacgcc
      781 ggcatcgctg agcgcgccgt tgacggtcgc gttgacggag tcgtcgattg agtcgagcac
      841 ctcgggcccg gtcggtgaca atgcgccgaa gcgggcgaaa gaaaacagca agaagaacac
      901 gacgctcgtc acgatgtaca tgcgaaacgg cggcgcgtag cgcacgcgct tgccctcgag
      961 gtacaaacgc gtcagccgac ccgggctcag cgtcagcacg acgaggctgc gccaaatgcg
     1021 cgagtcgaag ttgagcagat cgccgagggc gtcggccagc agatggccca gcgtcggaat
     1081 gcggacatcg cccttctggc cgcagacgcc gcagaacggt cccgcgagcg ccgcgtggca
     1141 gttctggcat tcgccggccg tactcacgcg cgcaagccta ctaccgttct gcgcttgcag
     1201 cacgcccgct cctcgcttgg aataggcgcc aaggatttcg acgggtagag acgactcatg
     1261 acatttctgg tgttctgcgt attcgacctg aagagcgcca cgcgcgaaga ctacctgtac
     1321 gcgtacatgg atctcgcgac gttggggctg cgtcaaaccg tgaagtcgga caacggcccg
     1381 agcttcaagc tgccggccgc tgccgtcatg gggacgttcg acggccgcag cgtcgacgac
     1441 gtgcgtggca acatcagccg caaagtccaa gggatcttca gagcccgcgg tttgaacggc
     1501 actttcttcc tcgtcgcaag ccacgattgg gcgtgcgcgg gagagtcgac gtagcgccgc
     1561 ccttcattga tctgaagttc gccgtggccg acaatccgcg ccgatagcca ccgggggagc
     1621 gcagcaatga gacacctgat cttagtcgct acgttggcgg cgctcgcggc attctcgagc
     1681 ggcgcgcagc aagcgcaagc gcagcgcggc cagaatgagg ccgccacccc ggcggatccc
     1741 ggcgcgggct ttgggccgca gcagctcacg acgttcaagg tccgcgacaa cttctacatg
     1801 atccgcagcg gccaatccgg caattgctcc gtgctgatcg ccgacgacgg cgtcgtgctg
     1861 atcgacaaca agttcgagat ggaccacgcc ggcatcatga ccaagctgcg cgagctcacc
     1921 gacaagccgg tgctctacgt catcaacacg cacatgcacg ccgaccattc gggtggcaac
     1981 gcgaccaagc aggcattggg tgcgaaggtc atcgcgcacg agaacgcccg cttccaaatg
     2041 gccatgacgc agacgacggg cctgccgaac ctgacgctcg aggaccacat gcgcctctat
     2101 cacggcgagt tcgttctcga cctctactat cttgggcgcg gccacacgga cggcgacatc
     2161 gtggtccatt tgcccgagca gaagatgatc ttcatgggcg acctgttcgc gacctgggat
     2221 ccgtacgtga cgctgatcca ctatgcagcc ggcggcagcc tgcgcgagtg gacacgaacg
     2281 ctcgaccgcg cgctcgcgct gccgttcgat accgtgattc ccggccacgg cggggatacg
     2341 gaccgcgcgc atctcgagac ctaccgcgcg cagctcgttc gcatgcagga catggtgcgc
     2401 gagatgaacc gcacgaagaa aacgcgcgcc gacattcaga agatgctcga ggtggagttc
     2461 ggctggagcg gtttcgtgac gaacctcggc ctcgacggcg tcatcggcga gatgcagtga
     2521 cgctcgcgta gccgccgacc cacgtgatcg ccagcgattt cgtcccgcct ccgcgtcggc
     2581 cgttcgcgcc gtggctgcgc tacgcgtcgt acgtgccgct gtacgggctg gcactgccga
     2641 tcgtcaagac gctcgaggcg ttcggggctt ggccgcaagt catgagccgg cgcgctcacc
     2701 gcgatgcctc gccgtttgcc gactatcgac cctcgggcgg cgacgtgttc gtctgcgcct
     2761 acttcaagtc cggtacgacg tggacgctac agatggcgac gcaaatcgcg tttcgcggtc
     2821 aggccgagtt cgcgaacatt catcatgtcg tgccctggcc cgacgtgccg atgccgccga
     2881 tgcagcgcat gatggttccg ctcgccgatc catcgccgct cgcccgctcg ccgacgggcc
     2941 gccgcgtcat caagacgcac ctgacgcgcg cggagattcc gttcgttgcc gaagctcgct
     3001 acatcgccgt cgtccgcaat ctcaaggacg tctgcgtgtc gggctatcac ttcctgaagt
     3061 cgatggcctt cggcccgctg atgccgtcgg tgaaccgttg ggtcgactac ctgctgtcgc
     3121 ccgactttcg gccgccatgg gccgagcacc tcgctagtta ttgggccgtg cggcacgagc
     3181 cgaacgtgtt gttcctgact tacgagcaca tgaagcaaga ccacgtcggt gccgtgcgca
     3241 agatggccga gttcatgggg atcgagctga cgctcggtga gctgcaatcc gtcgtcgagc
     3301 aatcgacgtt tgcggcgatg aaggccggcg gccttcgctt cgagcccggg cgcgtcgtgc
     3361 cgtggggcca agaacgctcg atgctgcggc gcggcgagag cgggcggtcg agcgagctgc
     3421 tgaccccgga gcaacaagcg cgcatcgacg actggtgccg cgccgagctc gcgcggttgc
     3481 aatgcgactt cccctacgac gaagccttcg ggcgctgacg cgcgagctac acgacgacgc
     3541 tactcggcga gaaagcgttg cgcgttgtcg cggctgcacg gcgtcgccgt gaattcgacg
     3601 tcggggcgat agagccagtc ggccgtacct gtgacgcgct gcttcaagaa ctcggggtcc
     3661 tcgagctcga agctgtaggt caggccgccc tgcgcgttca aagcgaatcg ttcgacgaga
     3721 tgcttgcgcg tacccgacgg cagccccgca ccgttgccga tgcggtgcgg cgtgaagttg
     3781 gccgtgtcga cgacgagcac accattgtcc cagcggccga tcgaatgtcc ctgcacggcc
     3841 ggcgctgcgc cctcgtgcgt cgctgcgtcg agatgcacgg tacgatcgac ggctgcatcc
     3901 tcgccgcgaa tcacgacggc atccgcacgt acgtcgatgc tacggaagcc cggcaaaatc
     3961 atgtacacgg gtatcgtgaa cggcacgcac tcgagcccgg ggttggccgc atcgcggaac
     4021 tcggcgacgg ccgcagcacc ctgcggcgtt ccaagctgcg cgactccggg cccaaagaag
     4081 ttgccgaacg caggccctac cgaggacgac caggtgccgg cgaggctcgt cgcccgaaac
     4141 gtctgcgacg gagccgatgg cggtgccgcg acggcccctt tcgcgtcgta gagcatcgcc
     4201 gccgcatcgg ctcggcgcac ggcttgtagg aacagcaccg tgcgcgccgg attgcggccc
     4261 gggtggcccg ttacaacgac gcgatcgccg agcgcgaagg tctgtttcga ccaaccgtat
     4321 tgcttcatcg cggtcgacgg gaacgcttcg acgacccaaa ccttgtcgct gccgttctcg
     4381 cgcacggaga gatacacgtg cgggttaccc cactcgtatt ccgcgatcgt gccctcgacg
     4441 gtgtgctgcg actgcatgtc gtagccggcg gggctgtggt gcgctagcgc gccgcgcgcc
     4501 agcagcaccg ccgcggccgt agccgcgacg tgcgctgcac gaacgtgcgc gtggcgcatg
     4561 gttgctctac tggcgctgcg cagctgccgg agcggcagca ggagccggcg gcacgacggg
     4621 cgtcaggtcc tgacagacct cggcctcgta cggcaccgtc gacacgagca tcgtcgtgct
     4681 cgacgtgtac ggcgccgtga agaactgcgc gtcctcggca gtcacgtcac gcttcagcgt
     4741 gttcttcgcc ggatcgagcg agaaacgctc cacgacgtgc agcgccgcac tatgcggcgt
     4801 cgtgccgacg agcgtgcccg gcagaaagcc gaccgtgtcg acgacgagca cgtcgttctc
     4861 ccacttgccg atggagtgcc ccgcgcggct cggcgccacg ttggccggat gcgccgccat
     4921 gccgacgttg acgatgcgcg acatgcccgt gcgcccgtac tgaatcgtga tcgcgttcgg
     4981 agtctgcgtg atgcggttga cgggctcgcc accccaatcg gagacgatgc tgcccggagc
     5041 gcacgcgcgc tcggctcgcg acagctgcgg taatgccttt tgcgcggctt cgccggccgg
     5101 cgtcaaagcc acagccgggc gcccgaacgc gccaccgcca ccgccggcgc ccgccggagc
     5161 cgtgcgcggc gtttgcgcct ccggcgtgcc acgcgcaccg ggaatttcgc gttggccttc
     5221 gggcacggca ccgcgtgcga agttgccgac cgtgctcagc ggcacgagcg tgccgtcgcg
     5281 gcccgcggcg tccgtcatca cgagctgctc gacggcccag tcgccggcga ggttcggctt
     5341 gccgtcggcc gtgcgcgccg cgcgcgtcgt cggcgcctgg gccgcaatac gctgcccgta
     5401 gcgatcgagc accgtgccgt ctgcgaaggt cagcgtgctc gtgtagcaag cgtccttctc
     5461 gttccgatcc ggcgagccct ggatcgtgat cttcgtgccg acggcgaaca tctcgggcgt
     5521 ccagccggag cgccgcaagg tcgtggcgga gcgcagctcg cagcgccgct gcgtgaccgt
     5581 gccgtcgggg ttcgtctggg tgaaatggac ccaggagtgc gggttgacga aatcgatttt
     5641 ggtcacgacg ccggaaaccg tgatgtcctc gtccatggcg aaattgccga agccgtgatg
     5701 ggccaaggcg acaggcgctg cggccaggct cgcgatgagt aggccggcac cgagtgtttg
     5761 gaattgctgc ttcacgcttc cccccgaaag aaaaaacgaa taggcgtgtg gaacggcgcc
     5821 tattatccgc agcggacagc gacgcgtcga tgctcgcgca gcgttcttga ggttgacggc
     5881 tgcccatgga ggctcgtagc gttcacttgt ctgcagttcc gactcggggg gcgcacatgg
     5941 accgtcgtat tcggtatctg gcagccgccg ccgctctcgc ggcaccgctc ttgatcgccg
     6001 aaatgctcgc cgcgcagccc ggcgaagcgg cctggaccgc tgcgcggaca caagcgggcc
     6061 agccggacct gcaggggcac tggacgaacg acacctacac accgctggag cgtcccgccg
     6121 agcttggcga caaacaggtg ttcacgcccg aggaagcggc cgcgttcttg aggtcgcgcg
     6181 tcgacgacct caatgggcaa gccgccgacg acgtccatta cgacgacgcg atctggcagg
     6241 ccgaaaacta cgacaaggtc gcgcatctgc acacgtcgtt ggtcctcgag ccgcacaacg
     6301 ggcgcttacc caagttgacg gcgcacggcg aggatcgact accgcggcaa cgcgccatgc
     6361 agcgagcgac gtccgccgac agcgcgcaga gccgctcgct cgcggagcgc tgcatctcat
     6421 ggggcaacgt cggaccgccg atggtgccgc cgacctacaa cgcgaatctg cagatcctgc
     6481 agagccgcga catcgtcgta attcggcacg agatgatgca cgacgtccgc acggtttatc
     6541 tggaagggcg ggcgcatccg ccggccgagc tgcaatggct ggccggtcat tcgatcggcc
     6601 gcttcgaagg cgacaccctg gtcgtcgaca cgacgaactt cacgccgctg acgaacttcc
     6661 gcggctcccc gcagaacaca cggcaagata tcttcgccag cgagcacctg cacgtcgtcg
     6721 agcgcttcac tcgcacgagc gacgacaaca tccactacga gttcaccgtc gacgatcccg
     6781 agacctgggt cgagccatgg tccggcgaga tggagattcg gcgcaccgaa gggccgatct
     6841 acgagtacgc gtgccacgaa ggcaacttgg gattggcgaa catcctacgc gccgcgcgcg
     6901 tcgcagaacg gcaggcgaca cgacaatgag tgaagcctgt ccgccgcggt cgttgtacga
     6961 cctgacggat gcagtgctgt tcgtcgggct cgagcccggc gaacgcggcc aacatcggtt
     7021 ctactgctag cgcgacccgc tcggcggccc cgccgggatg tcggccggcg cctcgcgccg
     7081 caagaacttc cactcgccgt tctcgcgcac gaactgatcg tcgtagtggc cgagatagac
     7141 gagcttcggc gcgttgtcgg tgccgggcgt gatgtagatc cacttcgtcg tcgcggacgc
     7201 atggtcgccg tcgagcttga tctgatcgtt cgtcagcacg tggtatgtgc ccg
//
LOCUS       DATLEE010000411         2542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4931336, whole genome shotgun sequence.
ACCESSION   DATLEE010000411 DATLEE010000000
VERSION     DATLEE010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4931336"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VM692_08825"
     CDS             <1..849
                     /locus_tag="VM692_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52312.1"
                     /translation="ELAQGLSSEGATSALLTRAELQLASGDATAAVETLRMLELEKAD
                     QPAATALLARAYRALGDRTQLTSLLPRLARAPLPAAEREELAKLALEDELARADLTDE
                     RLAQVWASVPSELRTSPALIAERARALDRLGHGEEAERELCAALKRDWHAALVQTFGE
                     VRGGDAVKQLKQAETWLKTYAEDATLLLAAARLSMANELWGKARSYLESSLALAATPD
                     AYALYGRLLTQLGEDDGALSAFRSGLSLASPVAAEPQVRRPLRSLAPPPAQDAERSDA
                     EVKAPG"
     gene            complement(853..1500)
                     /locus_tag="VM692_08830"
     CDS             complement(853..1500)
                     /locus_tag="VM692_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HUQ52313.1"
                     /translation="MRHILVTNAKGGCGKSTIATSLAAYFASEGYATALVDFDPQASA
                     LSWLEQRPKDFAPITGVAGFESGLKNVPRNAEYVIIDSPAGSYGKNLTELVRKAETIV
                     VPVLPSPIDIKAAERFVEELLTINKVADKKAKAALVANRVRENTLIFEELDAVLSRLK
                     VPYVAQFREAQNYNRAYQRGLGVHELPPYLAWPDWEQWDPLIEWLDSRRSQPKTA"
     gene            complement(1607..>2542)
                     /locus_tag="VM692_08835"
     CDS             complement(1607..>2542)
                     /locus_tag="VM692_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUQ52314.1"
                     /translation="QQVVGRNKIIMRWLAHRGGATDYQAFRAQHPNERFPVAVSLGAD
                     PATILAAVTPIPNTLSEYAFAGLLRSGRSEVAKCLGSDLLVPATAEITLEGYLEPGEE
                     ALEGPFGDHTGYYNEAQRFPVMTIERITHRREPIYHTTYTGRPPDEPAILGLALNDVL
                     VPILQSQFPEIVDFYLPSEACSYRVAVVSIRKEYPGHAKRVMFGVWSFLRQFMYTKIV
                     IVVDADIDARKWADVIWAISTRVDPKRDSVLVESTPIDYLDFASPVAGLGSKLGLDAT
                     TKWPGEVDRQWGRPITMRPDVVAKVDALWSELGLG"
BASE COUNT          416 a          833 c          869 g          424 t
ORIGIN      
        1 gagcttgcgc aggggctgtc gtccgagggc gccacgagcg cgttgttgac gcgcgccgag
       61 ctgcagctcg cctcgggcga cgcgacggcg gccgtcgaaa cgctgcgcat gctcgagctc
      121 gagaaggccg accagcctgc cgcgacggcg ctcttggccc gcgcctaccg cgcgctcggc
      181 gaccgcacgc agctcacgtc gttgctgccg cggctcgcgc gcgcgccgct gccggcggcc
      241 gagcgcgagg agctcgctaa gctggcgctc gaggacgagc tcgcgcgcgc cgatctgacg
      301 gacgagcggc tcgcgcaggt ttgggcctcg gtgccgagcg agctgcgcac gtcgccggcg
      361 ctgatcgccg agcgcgctcg ggcgctcgat cggctcgggc acggcgagga ggccgaacgc
      421 gagctgtgcg cggcgctgaa gcgcgactgg cacgctgcac tcgtgcagac cttcggcgag
      481 gtgcgcggcg gcgacgccgt gaaacagctc aagcaagcgg agacgtggct caagacgtat
      541 gccgaggacg cgacgctgct gctggcggcc gcgcgcctgt cgatggccaa cgagctgtgg
      601 ggcaaggcgc gcagttatct cgagtcgagc ttggcgctgg ccgcgacgcc cgacgcctac
      661 gcgctgtacg gccggctgct gacgcagttg ggcgaggacg acggcgcgct cagcgcgttt
      721 cgttcgggcc ttagcctcgc gagccccgta gctgccgagc cgcaggtgcg gcggccgctg
      781 cgtagcctcg cgccgccgcc cgcgcaggac gccgagcgca gcgacgcgga agtcaaagcg
      841 cccggctgag cgtcaagcgg tcttcggttg cgagcgccgc gagtcgagcc actcgatcag
      901 cggatcccac tgttcccaat cgggccacgc gagatacggc ggcagctcgt gcacgccgag
      961 gccgcgctga tacgcgcggt tgtagttctg ggcttcgcgg aactgcgcga cgtacggcac
     1021 tttgaggcgc gacagcacgg cgtcgagctc ctcgaagatc agcgtgttct cgcgcacgcg
     1081 gttcgcgacg agcgcggcct tcgccttctt gtcggccacc ttattgattg tgagcagctc
     1141 ctcgacgaag cgctcggcgg ccttgatgtc gatcggcgac ggcaacacgg gcaccacgat
     1201 cgtctcggct ttgcgcacga gctccgtcaa attcttgccg taggagccgg ccggcgaatc
     1261 gatgatcacg tactcggcgt tgcgcggcac gttcttgagc ccgctttcga agcccgccac
     1321 accggtaatc ggcgcgaaat ctttcgggcg ttgctcgagc caagagagcg cggaagcctg
     1381 cggatcgaag tcgaccaatg ccgtcgcata accctcggac gcgaaatagg ctgccaggct
     1441 cgtggcgatg gtgctcttac cacagccgcc cttggcgtta gtgacgagaa tatgccgcat
     1501 tggatctccg ctcgacttgt tcgatttgtt cttggttacg aattcggggg atgcgcgcga
     1561 aagggcgtaa cttagccagg cttatgacaa atgtccattg ccagaactaa ccgaggccga
     1621 gctcgctcca gagcgcgtcg actttggcga cgacgtcggg tcgcatcgtg atcggccggc
     1681 cccattgccg gtcgacctcg cccggccact tcgtcgtcgc atcgagcccg agcttcgagc
     1741 ccaggcctgc gaccggcgaa gcgaaatcga ggtaatcgat cggcgtcgac tcgacgagca
     1801 cactgtcgcg cttcggatcg acgcgcgtcg agatcgccca gatcacatcg gcccatttgc
     1861 gcgcgtcgat gtccgcgtcg acgacgatga cgatcttcgt gtacatgaat tgccgcaaga
     1921 acgaccagac gccgaacatc acgcgtttcg cgtggcccgg atattccttg cggatgctga
     1981 cgacggcgac gcggtacgag cacgcttcgc tcggcagata gaagtcgacg atctcgggaa
     2041 actgcgattg caggatcggc actaacacgt cgttcaacgc gagacccaga attgcgggct
     2101 cgtcgggcgg ccggcctgtg tacgtcgtgt ggtagatcgg ctcgcggcga tgcgtgatgc
     2161 gctcgatcgt catgacggga aagcgctgcg cctcgttgta atagcccgtg tggtcgccga
     2221 acggtccctc gagcgcttcc tcgccgggct cgagatagcc ctcgagcgtg atctcggccg
     2281 tcgccggcac gagcaggtcg gaccccaagc acttcgcgac ctcggatcgg ccgctgcgca
     2341 gcaagcccgc gaacgcgtac tccgacaacg tgttcggaat cggcgtgacg gcggcgagaa
     2401 tcgtcgcggg gtcggcgccc aagctcacgg caaccggaaa cctctcgttg ggatgctggg
     2461 cgcgaaacgc ctgatagtcc gttgcaccgc cgcggtgcgc gagccatcgc atgatgatct
     2521 tattgcggcc gacgacctgc tg
//
LOCUS       DATLEE010000412         3969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_464523, whole genome shotgun sequence.
ACCESSION   DATLEE010000412 DATLEE010000000
VERSION     DATLEE010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464523"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VM692_08840"
     CDS             complement(<1..1435)
                     /locus_tag="VM692_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUQ52315.1"
                     /translation="MWLSDTSVKRPVFATVLSMLLVAIGALSFRDLTVRENPDTVSPT
                     VQVQVGYPGANAEVIETRITQVLEAELSGIEGVKNIRSQSRDGQSSLTVEFYLDRNLD
                     EAANDVRDRVSRVSRRLPDDADQVSVQKADADTQPIMWLTLASEGMSLMDLTDYMERY
                     LLDRFATIPGVSQINLFGSGGPSMRVWIDRQALTARNLTVTDIENALTRENVELPAGR
                     LESRDLDFQVRIARNFQTADNFRNLVIAQGEDGHLVRLGEIAKVEIASRQTNRIFRTN
                     GALTTGFGIIKASTANTVEVLDDVKAEVEQVNKDLPEGMELITSGDESLYIRAAIEEI
                     YWTIGITTALVGFVIFVFLGSLRATLIPLVTIPICLVATFSVLAAFGYSINLVTLLAL
                     ALSIGLVVDDAIVVLENAHRRIEENNEAPLLAAFNGTRQVAFAVIATTVVLVSVFAPV
                     AFLRDSIGRVFAELAVTIAAAVIFSAVL"
     gene            complement(1449..2627)
                     /locus_tag="VM692_08845"
     CDS             complement(1449..2627)
                     /locus_tag="VM692_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUQ52316.1"
                     /translation="MKNALLIVLAFALGGGAMWYVTKERGTSAATGPAAAAGGRPGGG
                     PPGGGGFGGFGGGRPQAQLPLVTVGHAKRDQIYDVVEALGTAQANESVTLTAKVTDSV
                     RRVNFEDGDYVEAGTVLIELTNQEEEALLAEARANLDDSQSQLRRLEDLSARGLTSAS
                     ELDVARSRAGAQQARFNSIVARLRDRLILAPFSGLLGFRQVSPGTMLSPNTAITSIDD
                     ISTIKLDFTVPESAIGTMTPGAKVIAKSVSFPDRDFQGTVRTVGSRVDPVTRAITVRA
                     HVENKDRALRPGMLLTVAVVTAERTALVVPEGSVFQVQNRAYVYRVDGDTVHQQQIEV
                     GGRRFGVVEVLSGLQEGDPIVVEGIIKLREGARVRYEEAAVSESSQGSAQSRRGDARS
                     "
     gene            2856..3782
                     /locus_tag="VM692_08850"
     CDS             2856..3782
                     /locus_tag="VM692_08850"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HUQ52317.1"
                     /translation="MSTQVAPRTASHDALVSITAHVPEDAMSAGLLGTERTGHGVRIR
                     EDGLIVTIGYVVHEAENLWIGTRNTVVPGFVVGYDFDSGLALVKPSLPLHGRSIELGR
                     ADSLVVGDSVTVLSSGGKDPSIDARVVAKQEFAGRWEYVLDQAVFTTPPHDSWSGAAL
                     VDSDGKLCGLGSLVIQGFETRAGKCDVNMFVPIELVAPIVDEIVEHGRRSAPPRPWLG
                     MLVHDDQHDLTVVGVYRNCPADKAGLRPGDVIVGIDDEPVVGLANMFRRVWSLGSAGV
                     DVPLNVLRNAERMQLKVRSGDRAGFQRKGTLQ"
BASE COUNT          686 a         1312 c         1293 g          678 t
ORIGIN      
        1 ccagcacggc ggagaagatc acggccgccg cgatcgtgac ggcgagctcg gcgaaaacgc
       61 ggccgatgct gtcgcgtaag aaggcgacgg gcgcgaacac ggacacgagc acgacggtcg
      121 tcgcgatgac ggcgaatgcc acctgacgcg tcccgttgaa cgcggccagc agcggcgcct
      181 cgttgttctc ctcgatgcgt cgatgcgcgt tctcgagcac gacgatcgcg tcgtcgacga
      241 cgagaccgat cgagagcgca agcgcgagca gcgtgacgag attgatcgaa tagccgaacg
      301 ccgcgagcac cgagaacgtc gcgaccaaac aaatcggaat cgtcacgagc gggatcagcg
      361 tcgcgcgcag cgagcccagg aacacgaaga tcacgaagcc aacgagcgcc gtcgtgatac
      421 cgatcgtcca atagatttcc tcgatcgctg cgcggatgta caacgactcg tcgccgctcg
      481 tgatgagctc catcccttcg ggcaggtctt tgttgacctg ctcgacctcg gccttgacgt
      541 cgtcgagcac ctcgaccgtg ttggccgtcg atgctttgat gatgccgaaa ccggtcgtca
      601 acgcgccgtt ggtgcggaag atgcggttcg tttggcgcga cgcaatctcg actttggcga
      661 tctcgcccaa acggaccaag tgcccgtcct cgccttgcgc aatgaccaga ttccggaagt
      721 tgtcggccgt ttggaagttg cgcgcaattc gcacttggaa gtcgaggtcc cgcgactcga
      781 gccgccctgc gggcagctcg acgttctcgc gcgtgagcgc gttctcgatg tcggtcacgg
      841 tgagattgcg cgccgtcaac gcctgccggt cgatccagac gcgcatcgac ggcccgcccg
      901 agccgaagag gttgatttgc gagacgccgg gaatcgtggc gaagcggtcg agcaagtacc
      961 gctccatgta atccgtaaga tccatgaggc tcatcccctc gcttgcgaga gtaagccaca
     1021 tgatcggttg ggtgtcggcg tcggccttct gcacgctgac ttgatcggca tcatccggca
     1081 gccggcgact gacgcgcgag acgcgatcgc gtacgtcgtt ggcggcttcg tcgagattgc
     1141 ggtcgagata gaactcgacg gtcaacgacg actggccgtc acgactctgc gagcggatgt
     1201 tcttgacgcc ctcgatgccc gagagctcgg cctcgaggac ctgcgtgatg cgcgtctcga
     1261 tgacctcggc attggcgccg ggatagccga cctgcacctg cacggtcggc gatacggtat
     1321 cgggattctc gcgcaccgtg agatcgcgga acgacagcgc gccgatggcg accagcagca
     1381 tgctgagcac cgtcgcgaag accgggcgtt tgacggatgt atcggacagc cacatcgcct
     1441 aaccctcgtc agctgcgggc gtcgccgcga cgcgactgcg ccgagccttg cgagctctcg
     1501 ctgaccgcgg cctcttcata gcgcacgcgc gcgccttcgc gcagcttgat gattccttcg
     1561 acgacgatcg ggtcgccttc ttgcaggccg ctgagcacct cgacgacgcc gaagcgacgg
     1621 ccgccgacct cgatctgctg ctgatgcacc gtgtcaccgt cgacgcgata gacgtaggcg
     1681 cggttctgca cttgaaacac ggagccctcg ggcacgacga gagccgtgcg ctccgcggtg
     1741 acgacggcca ccgtcaacag catgccgggc cgcagcgcgc ggtccttgtt ctcgacgtgt
     1801 gcacggaccg tgatcgcgcg cgtcacggga tcgacgcgcg agccgacggt gcgcacggtg
     1861 ccttgaaaat cgcgatccgg gaagctcacg ctcttcgcaa tgaccttggc acccggcgtc
     1921 atcgtgccga ttgccgactc gggcacggtg aagtcgagct tgatcgtcga aatgtcgtcg
     1981 atcgaggtaa tcgcagtgtt cgggctcagc atcgtgccgg ggctgacttg ccgaaagccg
     2041 aggaggccgc tgaacggagc cagaatgagc cggtcgcgca gccgcgcgac gatactgttg
     2101 aaacgcgcct gctgggcccc ggcccgcgac cgcgcgacgt cgagctcgga cgcggaggtc
     2161 aggccgcgcg cgctcaagtc ttccaaccgg cgcagctgac tctgcgagtc gtcgaggttc
     2221 gcgcgcgctt cggcgagcaa cgcttcctct tcttggttcg tgagctcgat gagcacggtg
     2281 ccggcttcga cgtaatcgcc gtcctcgaaa ttgactcgtc gcaccgagtc ggtgaccttg
     2341 gccgtcaacg tcacggactc gttcgcttgc gccgtgccga gcgcctcgac gacgtcgtag
     2401 atttgatcgc gtttcgcgtg gccgaccgtg acgagcggca gttgagcttg cggacgcccg
     2461 ccgccgaagc cgccgaaacc accgccaccc ggcgggccgc cgcctgggcg cccgccagct
     2521 gcagccgcgg gacccgtcgc tgcgctcgtg ccacgctcct tggtgacata ccacatggct
     2581 ccgccaccca aagcgaaggc gagcacgatc agcaaagcgt ttttcatgaa cgtcttccgg
     2641 gttccctagc gtagtgagct ctgcttccgt gccgagcatc gtcagaagcg cggatcgcgc
     2701 gtgccggaaa agtatgccac aggggtctgc aggcgccgga ttcgcgcccg cgattcggtc
     2761 tcattttttc taaaggttta aggggttaga cgcgactcgc gcagcgcgcg acacgcgact
     2821 cgcaccatga gcaccgcgac gctaccatta cgcgcatgag cacgcaggtc gcaccgagaa
     2881 ccgccagcca cgacgcgctc gtgtcgatca cggcccatgt gccggaggac gcgatgagcg
     2941 cgggcctgct cggcaccgag cgcacgggtc acggcgtccg gatccgcgag gacgggttga
     3001 tcgtgacgat cggctacgtc gtgcacgagg cggagaacct ttggatcggc acgcgcaaca
     3061 ccgtagtgcc gggcttcgtc gtcggttacg acttcgacag cggcctcgcg ctcgtcaagc
     3121 cttcgctgcc gctacacggc cgctcgatcg agctcggccg cgccgactcg ctcgtcgtcg
     3181 gcgactcggt cacggtcttg agcagcggcg gcaaggaccc gagcatcgac gcccgagtcg
     3241 tcgcgaagca ggaattcgcg ggccgctggg agtacgttct cgaccaagcg gtgttcacga
     3301 cgccgccgca cgacagctgg tcgggtgctg cgctcgtcga tagcgacggc aagctctgcg
     3361 gtctcgggtc gctcgtcatt caaggtttcg aaacgcgcgc cggcaagtgc gacgtcaaca
     3421 tgttcgtgcc gatcgagctg gtcgcgccga tcgtcgacga gatcgtcgag cacggccgcc
     3481 gctccgcgcc gccgcgccct tggctcggca tgctcgtgca cgacgaccag cacgacctga
     3541 ccgtcgttgg cgtctatcgg aactgccccg ccgacaaggc cgggcttagg cccggcgacg
     3601 tcatcgtcgg catcgacgac gagcccgtcg tcgggctcgc gaacatgttc cggcgcgtct
     3661 ggagcctggg cagcgccggc gtcgacgtgc cgctcaatgt gctgcgcaac gccgagcgca
     3721 tgcagctcaa agtacgctcc ggcgaccgcg cgggctttca gcgcaagggc acgctgcagt
     3781 agcccgcaag atcccgggta gaaacaagaa cggcggccct tggccgccgt tcgcgtctag
     3841 aagctgcggt gttgaattta ctcttgccga cgccgatacg gccgatggca accgccgcag
     3901 gtctgaccga cggcttgcac catgcctttc gcggcctcga acccttgcga ttgcgccgtt
     3961 gccgcgagc
//
LOCUS       DATLEE010000413         3614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3225069, whole genome shotgun sequence.
ACCESSION   DATLEE010000413 DATLEE010000000
VERSION     DATLEE010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3225069"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1221
                     /locus_tag="VM692_08855"
     CDS             109..1221
                     /locus_tag="VM692_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HUQ52318.1"
                     /translation="MTDKPGLDYAAAGVDYGTLDASKILAQKAARATAANLAAHGAAE
                     IEESRGESAYLFEVGGMLIASITECLGTKALVADAVRLYSGRSHYDTIAQDTLAMAVN
                     DLVTVGATPLSTHAYWSAGSSDWFADHERVGDLVRGWQGACDVCGMAWGGGETPALGG
                     VVEKERIDLAASCVGIVKGRDRLMLGEQLAVGDAIVLLASSGIHANGISLARKLAEQL
                     PEGYRTRLGDGRSFGEALLDPTVLYAPITEQLFAAGVRPHYAVNVTGHGWRKLMRHPA
                     ELRYVIRSIPPVPPVLQFLRTQGGMSARDAYGTFNMGAGFALFVPRAAVAATLAVAEH
                     AGLAAWHAGDVHSGPKSLVIEPLGLEYSAGDLHLRA"
     gene            complement(1367..3028)
                     /gene="murJ"
                     /locus_tag="VM692_08860"
     CDS             complement(1367..3028)
                     /gene="murJ"
                     /locus_tag="VM692_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HUQ52319.1"
                     /translation="MASADGEAGGSPEAVAPAARSGAAALFVGLGIFLSRVIGLVRER
                     VLATYFGTGLHADVFSAGLRLPNVLQNLLGEGTLSASFIPAYSALLGQGRTAEAGRVA
                     GAAFALLLSVAGAISLLGLALAPFLVSVFTPGFEGVRRELLIVVVRIVFPMTGVLVLS
                     AWALGILNSHRKFFLPYFAPVLWNAAIIGTLIAFAARLDLDALVVAAAWGALAGGALQ
                     FLVQLPAVLRLDREIRLSTGRNEPAFREAVRKAGPAIMGRGVVQLSGYLDLVLASLLA
                     IGAVARLRYAQTLYLLPISLFAMSVAAAALPELARDRDGAAGALRERTVAAVRRVAFF
                     VVPSFVAFVAIGDVLVAGLYRAGEFGAADVEVVWLILVAYSLGLLASTTTRVYQSAFF
                     ALRDTATPARVAFVRVLVALAAGAVLMVQFEPVTVLGRTIPAGMLASYDAAGLPLGPL
                     GLAAGASLGAWIEWALLKRALVARLGGVAVGAAAHARMFAAALAAGGAAYLAHRALAS
                     PQPPLAAALAAGVFGAVYFALAAALGLEQARSLPAALLRRLRRRR"
     gene            complement(3028..>3614)
                     /locus_tag="VM692_08865"
     CDS             complement(3028..>3614)
                     /locus_tag="VM692_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUQ52320.1"
                     /translation="VLWAIGRTPNVVDVGLESAGVKLDAEGFIAVDRFQNTSAAGVYA
                     IGDVTGQAELTPVAIAAGRRLADRVFGGMTDRHLRYDVIPSVVFSHPPIGTVGLSEAE
                     ARQRYPDEAIRIYKSEFVSMFYALMDVKPQTAMKLVCVGADERVVGCHVIGLGADEMI
                     QGFAVAVTMGARKRDFDETIAIHPTSAEELVTMR"
BASE COUNT          626 a         1288 c         1237 g          463 t
ORIGIN      
        1 ccctttccgc ggacgtggat gctccgaccg agcgcgacta ttttcggaga gacgagaaac
       61 cggcgtcaac cggaatacaa tgcgcgccat gagctcgctc tcaccttcat gacggacaag
      121 ccgggcctcg actacgccgc cgccggcgtc gattacggaa cgctcgacgc ctcgaagatc
      181 ctggcacaga aggccgcgcg cgcgacggcc gccaaccttg ccgcgcacgg cgcggcggag
      241 atcgaagagt cgcgtggcga gtcggcctac ctattcgaag tgggcggcat gctgatcgcc
      301 tcgatcaccg agtgcctcgg cacgaaggcg ctcgtcgccg acgccgtgcg cctgtactcg
      361 gggcgcagcc attacgacac gatcgcgcag gatacgctcg cgatggccgt caacgacctc
      421 gtcaccgtcg gcgcgacacc gctctcgacg cacgcgtatt ggtcggccgg cagcagcgac
      481 tggttcgcgg accacgagcg cgttggcgat ctcgtgcgcg gctggcaagg agcgtgcgac
      541 gtgtgcggca tggcgtgggg cggcggcgaa acgccggcgc tcggcggcgt cgtcgagaaa
      601 gagcgcatcg atctggcggc gtcgtgcgtc ggcatcgtga aaggccgcga ccgcctcatg
      661 ctcggggagc agctcgccgt cggcgacgcg atcgtgctgc tcgcttcgag cggcattcac
      721 gcgaacggca tcagcctcgc gcgcaagctt gccgagcagc tgcctgaggg ctatcgcacg
      781 cgcctcggcg acggccgcag cttcggcgaa gcgttgctcg atccgaccgt tttgtacgca
      841 ccgatcacgg agcaattgtt cgcggccggc gtgcgcccgc attatgccgt caacgtcacg
      901 ggccacggct ggcgcaagct gatgcgccat ccggccgagc tgcgctacgt gatccgcagc
      961 atcccccccg tgccgcccgt cctacagttc ctgcgcacgc aaggcggcat gagcgcccgc
     1021 gatgcgtacg gcacgttcaa catgggcgcg ggcttcgcgc tgttcgtgcc gcgcgcggcc
     1081 gttgccgcga cgctcgccgt cgcggagcac gccggcctcg cggcatggca tgccggcgac
     1141 gtgcactcag ggccgaagag tctcgtcatc gagccgctgg gcttggagta ctctgcgggc
     1201 gatctccacc tccgagcctg aggcatgcgc gcctatcggt cggtagtgct gttgctcacc
     1261 gcgctcgcgc agcctcggcc cgatttgccg tacacagcgg caaggtgacc gcgagaacgc
     1321 acgccgttat ctcgaagcgc gctgagtaga gccggcgcgc gactctctat cgacgacgtc
     1381 gcaggcggcg cagcagcgcg gccggaagcg agcgcgcctg ctcgagaccg agggcggcag
     1441 cgagcgcgaa atagactgcg ccgaatacgc cggccgcgag cgcggccgcg agcggcggct
     1501 gcgggcttgc gagcgcacgg tgcgcgagat acgcggcgcc tccggccgcc agtgcggccg
     1561 caaacatgcg cgcgtgcgct gcagcgccga cggcgactcc gccgagccgt gcgacgagcg
     1621 cgcgcttcag cagcgcccat tcgatccacg cgccgagcga cgcgccggcc gcgaggccga
     1681 gcggcccgag cggcaaccct gccgcgtcgt acgacgcgag catgccggcc gggatcgtgc
     1741 ggccgagcac cgtgacgggc tcgaactgca ccatcagcac cgcgcctgcg gcgagcgcga
     1801 cgagcacgcg gacgaacgcg actcgcgccg gcgtcgcggt atcgcgcagc gcaaagaacg
     1861 cggactgata cacgcgcgtc gtcgtggacg ccaacagtcc caaactgtag gcgaccagga
     1921 tcaaccacac gacctcgacg tcggccgcac cgaactcgcc ggcacgatag aggccggcga
     1981 cgagcacgtc gccgatcgcg acgaacgcga cgaagctcgg cacgacgaag aacgcgactc
     2041 gacgcacggc cgcgacggtg cgctcgcgca gcgcgccggc agcgccgtcg cgatcgcgcg
     2101 cgagctcggg cagagccgcg gccgcgacgc tcattgcaaa caggctgatc ggcaacagat
     2161 acagcgtctg cgcgtaccgc agccgcgcga ctgcgccgat cgcgagcagg ctggcgagca
     2221 cgaggtcgag ataaccggac agctgcacga cgccgcgacc catgatcgcg ggcccggctt
     2281 tgcgcaccgc ctccctgaac gcgggctcgt tgcggcccgt gctcaagcgg atctcgcgat
     2341 cgaggcgcag cacagccggc agctgcacga ggaactgcag tgcgccgccg gcgagcgcgc
     2401 cccacgccgc tgccacgacg agcgcgtcga ggtcgagccg cgcggcgaac gcgatcagcg
     2461 tgccgatgat cgccgcgttc caaagcacgg gcgcgaagta cggcaagaag aacttgcggt
     2521 gactgttcaa aatgccgagt gcccacgccg acagcacgag cacgcccgtc atcgggaaca
     2581 cgatgcgcac gacgacgatc aagagctcgc ggcgcacgcc ttcgaagccg ggagtgaaca
     2641 cgctgaccaa gaacggtgcg agcgccaggc cgagcaacga gatggccccg gccacgctga
     2701 gcagcagcgc gaacgccgcc ccggcaacgc gcccggcttc ggccgtccgg ccctgcccca
     2761 gcaacgccga gtacgccgga atgaacgacg ccgacagcgt cccttcgccg agcagatttt
     2821 gcagcacgtt cggcagccgc agacccgcgc tgaacacgtc ggcatgcagg cccgtgccga
     2881 agtaggtcgc gagcacgcgc tcgcgcacca agccgatgac gcggctcaga aagataccga
     2941 gcccgacgaa caacgccgca gcgccggagc gcgcggccgg cgccaccgcc tccggactac
     3001 cgcccgcctc gccgtcggcc gacgccatca gcgcatcgtc acgagctctt cggcgctcgt
     3061 cggatggatc gcaatcgttt cgtcgaagtc gcgcttgcgc gcgcccatcg tcacggccac
     3121 ggcgaaccct tggatcatct cgtcggcgcc gaggccgatg acgtgacagc cgacgacgcg
     3181 ctcgtcggcg ccgacgcaga cgagcttcat cgcggtctgc ggcttgacgt ccatcagcgc
     3241 atagaacatc gagacgaact cggacttgta gatgcgaatc gcctcgtcgg gataacgctg
     3301 ccgcgcctcc gcttccgaca agccgacggt gccgatcggc ggatgcgaga acacgacgga
     3361 cggaatgacg tcgtagcgca gatgccggtc cgtcatgccg ccgaacacac ggtccgcgag
     3421 ccgccgtccg gccgcgatcg cgacgggggt cagctcggct tggcccgtga cgtcgccgat
     3481 ggcgtagacg ccggcggcgc tcgtgttttg gaagcggtcg acggcgatga agccttcggc
     3541 gtcgagcttg acgccggcgc tctcgaggcc gacgtcgacg acgttcggcg tgcggccgat
     3601 cgcccacagc accg
//
LOCUS       DATLEE010000414         3255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7682355, whole genome shotgun sequence.
ACCESSION   DATLEE010000414 DATLEE010000000
VERSION     DATLEE010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7682355"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VM692_08870"
     CDS             complement(<1..552)
                     /locus_tag="VM692_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUQ52321.1"
                     /translation="MSAQQLRKSHTSDDFGHFIKGWIRNPLAMGALAPSSKSLAKIMA
                     RDVGTGSRVLELGAGTGTVTEALLASGVVPEDLYLVERDRHFVKLLQRRFPRCHVVAT
                     DALELDLPSDTEGTFDFVVSGLPLLCFSPDKRYRILQRALQLLRPKGHLHQFTYAGRC
                     PVDKDLRALLRVDSTLLGIAPLNL"
     gene            complement(603..1199)
                     /locus_tag="VM692_08875"
     CDS             complement(603..1199)
                     /locus_tag="VM692_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HUQ52322.1"
                     /translation="MRAKLIVLVAVASICGCGRGADPAADSASPAGTWQTLFDGTSLA
                     GWMPVGDANWQLMDGAVAADSGTGFLLTAASYADFDLDLEFWVTPDANSGVFIRCANP
                     SEIGAATCYEVNIFDQRPDPTYRTGAIVEVAGPKVMLETGDRWNRYEISAHGPHLVVK
                     LNGQTTVDTNDDKLAAGPFALQYGAGRVMFRNVRIRAD"
     gene            complement(1309..2670)
                     /locus_tag="VM692_08880"
     CDS             complement(1309..2670)
                     /locus_tag="VM692_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUQ52323.1"
                     /translation="MANVLLVDDEPDFVAAATEMLQLHGHRVESAERLESARRLLGGA
                     AVPDVLLLDLMLPDGNGLELLEELRASPVKRVVFITGHPGIKSLIKNLSGPSVSYLTK
                     PIGSSELLRTVESDSPAPESTPTIEGSGNLHFGCLIGESPAMLSVYEQIEKVARTPSP
                     VLILGETGTGKELVAESIHRASTRPGQFVAVNCGSLSRELAASELFGHEKGSFTGAVR
                     RHPGFFKRADGGTLFLDELAEMPLELQPHFLRVLETGSVLPVGSEREVPVDARTVAAT
                     NRDPKKAIADKALREDLYYRLSVFPITLPPLRARIQDVPLLVEHFLSQAQTAGAQGRR
                     FSAEDSARLRGYHWPGNVRELRHVVLRAAIMSDPNDSLLRLPERFDSPFNEPNGQQGL
                     AIGRSIRDVERELIMRTLEHLNGDKRAAAETLGISLNTLYNRLNAYEAAGISTPAAPP
                     SLP"
     gene            2954..>3255
                     /locus_tag="VM692_08885"
     CDS             2954..>3255
                     /locus_tag="VM692_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52324.1"
                     /translation="MLELEGTSPRSFAAATVAALLFLVMVAAVTYRVLGLEAASALAA
                     AGGVALILGLLFAASKRLRRADDGLRLGERALEAVPHALFVVDALQPGRPNRYVNPA"
BASE COUNT          585 a         1049 c         1054 g          567 t
ORIGIN      
        1 caaattcaac ggcgcgatgc cgagcaaagt gctatcgacg cgcagtagcg cgcgcagatc
       61 cttatcgacg gggcaacggc cggcgtaagt gaactggtgc aaatggcctt tgggtcgcaa
      121 gagttgcaac gcgcgttgca agatgcggta gcgcttgtcg ggcgaaaagc agagcagcgg
      181 caggccgctg acgacgaagt cgaaggtgcc ttcggtgtcg gacggcaagt cgagctccaa
      241 tgcatcggtg gcaacgacgt ggcagcgcgg gaagcgccgc tgcaacagct tgacgaagtg
      301 ccggtcgcgc tcgacgaggt ataggtcctc gggcaccact ccgcttgcga gcaatgcctc
      361 ggtcacggtg ccggtgcccg cgccgagctc gagcacgcgt gagcccgtgc ccacgtcccg
      421 cgccatgatc ttcgccagcg atttactgct cggggcgagc gcgcccatcg caagcggatt
      481 gcggatccag cctttgatga agtgaccgaa atcgtcggat gtatgggact tcctgagctg
      541 ttgggcagac attcgatcgc ctatcctcgc aaagcggtta gaacgggctg cgaaaccgca
      601 ggctaatcgg cacggatccg cacgttgcgg aacatcacgc gcccggcgcc gtattgcaga
      661 gcgaacgggc cggctgccaa cttgtcgtca ttagtgtcta ctgtggtctg tccgttcaat
      721 ttcacgacga gatgcggacc gtgggccgag atttcgtagc gattccaacg gtctcccgtc
      781 tcgagcatca ctttcggccc cgccacctcc acgatagcgc cagtgcgata cgtgggatcc
      841 gggcgctgat cgaatatatt gacctcatag cacgtcgcag cgccgatctc gctcggattg
      901 gcgcaacgga tgaacacgcc gctgttcgcg tcgggcgtga cccagaactc gaggtcgagg
      961 tcgaagtctg cgtacgacgc cgccgtcaag aggaagcccg tgccggagtc ggcggcaacc
     1021 gcaccgtcca tgagctgcca gttcgcatcg ccgacgggca tccatcccgc gaggctcgtg
     1081 ccgtcgaaca gcgtttgcca cgtaccggcc ggactggccg agtcagccgc cggatccgcg
     1141 ccgcgtccgc agccgcagat actcgcgacc gctacgagga cgatcagctt tgctcgcatg
     1201 taccccccgg ctatacaacg gctgggcgac gcctgcttgg ggcagccggc ctggttgccg
     1261 cggagcttag cagcccacgg cggggaacac tatcgcagct ttccgccctt agggcagcga
     1321 gggcggcgcc gccggcgtcg agataccagc agcctcgtaa gcattgaggc ggttgtaaag
     1381 cgtattcagg ctgatgccga gcgtctccgc tgcagcacgc ttgtcgccgt tcaagtgctc
     1441 gagagtccgc atgatcagct cgcgctcgac gtcgcgaatc gaacgaccga tcgcgagccc
     1501 ttgctgaccg ttcggctcgt tgaacgggct atcgaaacgc tcgggcaaac gcagcagcga
     1561 atcgttcgga tcggacatga tggcggcgcg cagcacgacg tgccgcagct cgcgcacgtt
     1621 gccgggccag tggtacccgc gcaggcgcgc ggaatcttcg gctgagaacc tgcggccttg
     1681 ggcacccgcc gtttgcgctt ggctcaaaaa atgctcgacg agcaacggca cgtcctggat
     1741 gcgagcgcgc agcggcggca gcgtgatcgg aaacacgctg agccggtaat acaagtcttc
     1801 gcgcagggcc ttgtcggcga tcgccttctt cggatcgcgg ttggtcgccg cgactgtgcg
     1861 cgcgtcgacc gggacttcgc gctcgctgcc gacgggtagg accgagccgg tttcgagcac
     1921 gcgcaagaag tgcggctgca gctcgagcgg catctcggcg agctcgtcga ggaacagcgt
     1981 gccgccgtcg gcgcgcttga agaagccggg gtgcctgcga acggcgcccg tgaagctgcc
     2041 cttctcgtgc ccgaacagct cgctggccgc cagctcgcgc gataacgacc cgcagttgac
     2101 ggcgacgaac tgacccggac gcgtgctcgc acgatgaatc gattctgcga cgagctcctt
     2161 gccggtcccg gtctcgccga ggatcagcac gggactcggc gtccgggcga ctttttcgat
     2221 ttgctcgtag accgacagca tcgccggcga ctcgccgatc agacagccga aatgcaaatt
     2281 gccgctgccc tcgatagtcg gcgtggattc gggcgcagga ctgtccgatt caacggttcg
     2341 caagagttcg ctgctgccga tcggcttcgt caggtacgaa accgacgggc cgctcaagtt
     2401 cttgatcaaa gacttgatcc cgggatgccc ggtgatgaac acgacgcgct tgacggggct
     2461 cgcccgcaac tcctcgagca gctcgaggcc attgccgtcg gggagcatta gatcgagcaa
     2521 cagcacatcg ggcacggcgg cgccgccgag tagccgtcgc gcgctctcga gccgctccgc
     2581 actctcgacg cggtgcccgt gtagctgcag catctccgtc gcagccgcaa caaagtccgg
     2641 ttcgtcgtca accaacaaga cgttcgccat gaataccccg caaagatgat tcttcgtcgt
     2701 aggccggaaa ttaccggccg cgctcagggt cactagagcc tgaccgcatt ctacctctat
     2761 aaatcaaccg agccactatc gcagcgtgtc gccgcccgac gacggtaaac gcccctgaac
     2821 gagctgcgcg tagaatgctt agtatgccga cgtcggcgtg cggcgcccgc gcgactggca
     2881 cagtcgtcgc atgggagaag cgagtggttg cacgtcgtct tggcaacata attctttgga
     2941 cggatacgcg acgatgctcg agttagaggg cacatcgccg cgctcgttcg cggccgcgac
     3001 tgtcgcggcg ctgttgttcc tggtcatggt ggcggctgtg acgtatcgcg tgctcgggct
     3061 cgaggccgca agcgcgctgg cggcggccgg cggcgttgcg ctgatcctcg gcctcttgtt
     3121 cgccgcgagc aaacggttgc gccgcgccga cgacggcctc cggctcggcg agcgcgcact
     3181 cgaagccgtc ccgcacgcac tgttcgtcgt cgacgccctg cagccgggcc gaccgaatcg
     3241 ctacgtcaat ccggc
//
LOCUS       DATLEE010000415         2577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7682777, whole genome shotgun sequence.
ACCESSION   DATLEE010000415 DATLEE010000000
VERSION     DATLEE010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7682777"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VM692_08890"
     CDS             complement(<1..212)
                     /locus_tag="VM692_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52325.1"
                     /translation="MRLGSIVTAAGVSLLAAGSARAQAPSAGQQPAYSVASPPITSPL
                     SAPAGKDSGAIRTALPNGVNQGPFNPA"
     gene            311..1150
                     /locus_tag="VM692_08895"
     CDS             311..1150
                     /locus_tag="VM692_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52326.1"
                     /translation="MPFPIHRMRTHASSAALSVVLLCVAAAAHAQWLDYPTPGIPRTA
                     DGKPDLQAPAPRTPDGKPDFSGMWFANVPAKDYCKEKDCIQEERMAREQINLGIELEG
                     GLPYTDWSKAQMATRRANGGREDPHTYCMPPNFPRAWTLPQYIKVVQTPGLMVLLHEF
                     NGAYREIFLDGRPLPEDPNPTWNGYSTAHWDGGTLVIETNGLRDDMWLDIQGSPVTEA
                     ARVTERLTRGNFGLMQVEIAVDDPKAYTRPWAVTVEMAYQADTTMLEEICLDDEQDVE
                     LFK"
     gene            1147..1563
                     /locus_tag="VM692_08900"
     CDS             1147..1563
                     /locus_tag="VM692_08900"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52327.1"
                     /translation="MKRTVRRSLTAACIGLMLAGSTQAHHSQSEFDFDKTVEVEGTVT
                     KLEWKSPHGRLYVDVKNDKGETASWNFELPSPNTLMRRGWKRDALQAGDVVKVTGNPA
                     RNFPTIAIATFIRDASGTPLFTGTTPVFEPEASTQP"
     gene            complement(1564..1776)
                     /locus_tag="VM692_08905"
     CDS             complement(1564..1776)
                     /locus_tag="VM692_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52328.1"
                     /translation="MPKTQNEDDKKHGDKMAPLIERTQRGGGAGKRANDADAAQLQDD
                     DDEDMQNDAPDSGRDVGGGEEIKPVR"
     gene            complement(1805..2110)
                     /locus_tag="VM692_08910"
     CDS             complement(1805..2110)
                     /locus_tag="VM692_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52329.1"
                     /translation="MARRRGKRAERAPNTAQLRGAMRSGRTPSIDSAHEPAAASFDTD
                     DEAAGRPAQTAAIEQALANEGRVPPQVPGGARRSIAAPLWIFAAAMVAAVLAWILLS"
     gene            complement(2112..2552)
                     /locus_tag="VM692_08915"
     CDS             complement(2112..2552)
                     /locus_tag="VM692_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HUQ52330.1"
                     /translation="MNVRTFALIYGLVFIVAGLAGFIPGLVTPHDAVEHQLAVEQGAG
                     DLLGLFPVNLLHNLVHLVFGVWGLAVYRSTSNAIGYARVVAVVYALFVIMGFIPNLST
                     VFGLVPLHGNDIWLHAVLAAGAAYFGFLWHAEPRAASGPAPRGT"
BASE COUNT          464 a          879 c          826 g          408 t
ORIGIN      
        1 gccggattga acggcccttg gttcacgccg ttcggcagcg ccgtgcgaat cgcgccgctg
       61 tctttgccgg ccggcgccga gagcggcgac gtgattggcg gcgacgcgac cgaatatgcc
      121 ggctgctgac ccgcgctagg agcctgcgca cgagcgctgc cggcggcaag aaggctcacg
      181 cccgcggcgg tcacgatgga accgagtctc atgttttccc cctgaagcag ccaagccgat
      241 actacgttcg cgtgtatcat cccgcaaaac aatcgacaat atcgggcgac cacggtcgct
      301 acgagaacca atgcccttcc ccattcaccg catgcggacc cacgcgagct ctgccgcgct
      361 ctccgtggtg ctgctctgcg tagccgccgc agcacacgca cagtggctcg actacccgac
      421 gccgggaata ccgcgcacgg ccgacggcaa gccggatctg caagcgccgg cgccgcggac
      481 gccggacggg aagccggatt tctccggcat gtggttcgcc aatgttcccg cgaaggacta
      541 ctgcaaggag aaggactgca tccaagaaga gcgcatggcg cgcgagcaga tcaatctcgg
      601 catcgagctc gagggcggct tgccctacac ggactggtcc aaagcgcaga tggcgacgcg
      661 gcgcgcgaac ggcggccgcg aggatccaca tacctactgc atgccgccga atttcccgcg
      721 cgcttggacg ctaccgcagt acatcaaggt cgtgcagacg ccgggcctca tggtgctgtt
      781 gcacgagttc aacggggcgt atcgcgaaat atttctcgac gggcgcccgc tgcccgaaga
      841 cccgaacccg acctggaacg gctactcgac ggcgcactgg gacggcggca cgctggtcat
      901 cgagacgaac ggtcttcgcg acgacatgtg gctcgacatt caaggcagcc ccgtgacgga
      961 ggcggcgcgc gtgacggagc ggctgacccg cggcaacttc ggcctcatgc aggtcgagat
     1021 cgcagtcgac gatccaaagg cctatacgcg gccttgggcc gtcacggtcg agatggccta
     1081 ccaggccgac acgacgatgc tcgaggagat ttgcctcgac gacgaacaag acgtggagct
     1141 gttcaaatga aacgcaccgt gcgtcgctcg ctgacggccg cctgcatcgg tctgatgctc
     1201 gctggttcga cgcaggctca ccactcgcaa tcggagtttg atttcgacaa gaccgtcgag
     1261 gtcgaaggca cggtcacgaa gctcgaatgg aagagccccc acgggcggct ctatgtcgac
     1321 gtcaagaacg acaaaggcga gaccgcgagc tggaacttcg agctgccgag cccgaataca
     1381 ctgatgcgcc gcggctggaa gcgcgatgcg ctgcaagccg gcgacgtcgt caaagtgacg
     1441 gggaaccccg cgcgcaattt tccgacgatc gcgatcgcga cgttcatccg cgacgcgagc
     1501 ggcacgccgt tgttcacggg cacgacgccg gtcttcgagc cggaggcgtc gacgcagccc
     1561 tgatcaccgc acgggcttga tttcctcgcc gccgccgacg tcgcggccgc tgtcgggcgc
     1621 gtcgttctgc atgtcctcgt cgtcgtcatc ttgcagttgc gccgcgtcgg cgtcattggc
     1681 gcgtttgccg gcgccgccgc cgcgctgcgt gcgttcgatc aacggcgcca tcttgtcgcc
     1741 gtgcttcttg tcatcctcgt tctgcgtctt cggcatcgtt ctttctcctc ttcagtgcgc
     1801 cacatcagct aagcaaaatc catgcaagca ctgccgcgac catcgcggct gcgaagatcc
     1861 acagcggcgc cgcgatgctc ctgcgcgcgc cgcccggcac ttgcggcggc acgcgcccct
     1921 cattggcgag cgcctgttcg atcgcggccg tctgcgcggg acggccggcg gcctcgtcgt
     1981 cggtgtcgaa cgacgcagcc gccggctcgt gcgccgagtc gatgctgggc gttcgcccgc
     2041 tgcgcatcgc gccgcgcaac tgcgccgtgt tcggcgcgcg ctccgcgcgc ttgccgcgtc
     2101 gccgcgccac gtcaggtgcc ccgcggcgca ggtccgctcg cggctcgcgg ctcggcgtgc
     2161 cagaggaagc cgaagtacgc agcccccgca gcgagcactg catgcagcca aatgtcgttg
     2221 ccgtgcagcg gcacgagccc gaacacggtg ctcaagttcg gaatgaagcc catgatgacg
     2281 aacagcgcgt acacgaccgc gacgacgcgc gcatacccga tcgcgttact cgtgctgcgg
     2341 tacaccgcga gcccccacac accgaaaacg aggtgcacca ggttgtgcag aaggttcacg
     2401 gggaacagcc ccaagagatc gccggcgccc tgctcgaccg cgagctgatg ctcgaccgcg
     2461 tcgtggggcg tgacgagccc cgggatgaag ccggcgaggc ccgcgacaat gaataccagg
     2521 ccatagatca aagcaaaagt tcgtacgttc atatgccctc cttggcaacg aggccgg
//
LOCUS       DATLEE010000416         6198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10670562, whole genome shotgun sequence.
ACCESSION   DATLEE010000416 DATLEE010000000
VERSION     DATLEE010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10670562"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /locus_tag="VM692_08920"
     CDS             complement(<1..1806)
                     /locus_tag="VM692_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUQ52331.1"
                     /translation="MTTRQGFAIGVLTLLLAACGGGGGSEPSGAASESPAASEAGTVV
                     ATTTRYVESLPTYPTEPLPEGLVWETNLTDPIYASPEAKRGGEYRYWLLSFPLTLRLV
                     GPDSNDAFTSYMRANDAALTDIHPNTRKPIPQLATEWAFAPDGKTVYYRLDPSATWSD
                     GLPVTADDYVFTLEFMRSEHIVDPWYNDHYTKDIVAVRKHDDHTISIEGSTAKPRDEL
                     LFFYGLAPTPRQFHKLDAKWVEDYNWRIPPGTGPYQISRIEKGQYIEFKRNPSWWGDK
                     QRYFEHRYNVDTIRLTVIRDQNVAWEYFLRGELDTFNVVFPNFWHDKAQGELFDKGYI
                     GKYKYYTETPMPSYGLWLNMDDPLLGDRNVRLGLHHSMNVDLLLSGLLRGDYERLKQH
                     YEGYGDYTDTSIGPRPFDLAKADEYFKAAGFAMRGPDGIRVKDRQRLSFTISYGNNEL
                     TPRMVVLREEARKAGVELNLQVQDSAATFKQTLEKKHQIAFMAWSPQHIPAFWEHYHS
                     DNAHIPQTNNITATDDAELDKIIIEQRDAIDLPTRIRLSHEIQQRVWEIGAFIPLYKV
                     PYVREVAWRWVRLPETHGTRMTDPYLFDPISQGLRT"
     gene            complement(1833..2873)
                     /locus_tag="VM692_08925"
     CDS             complement(1833..2873)
                     /locus_tag="VM692_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52332.1"
                     /translation="MRLAPQTIKQWKRFRSIKRGYYSLIITLVLLALSLGAELLVNNR
                     ALVVKYEGDFYFPTYAAFIPGRTFGLDYDYETNYRELKAAFAQSDGNWVLLPPVPFNA
                     YETDFRTDAFPPYPPSFATRHYLGTDVTGRDIVARLFYGFRLATAFAVILLVCNYVVG
                     ISIGCAMGFWGGAFDLLFQRLIEIWSNVPFLYVIMIVASVIVPNFWTLVIAMVVFGWM
                     GMTWYMRTVTYKEKAREYVAAARALGASNTRIVFRHILPNTISIIVTFIPFSIAAGVT
                     ALTALDYLGFGLPPPTPSWGELLSQGTANLQAPWIVTSVVTAMTVVLLMVAYTGEAIR
                     EAFDPKQFSYYA"
     gene            complement(2886..3911)
                     /locus_tag="VM692_08930"
     CDS             complement(2886..3911)
                     /locus_tag="VM692_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HUQ52333.1"
                     /translation="MGTTSYFIRRLLLIVPTFIGITLLVFAVTRVVPGGPIERMMNQA
                     LMASGERGGAQRSVAGSTLSEAQLNQLRVYYGFDKPILVSYVDWLGKVLKLDLGRSTR
                     YNEPVWDTIKDRFPVSIFYGLATLVLTYAVCIPLGIAKAIRHHGLFDNVSSGVVFFGY
                     AVPSYVIGIALLVYFASQLGWFPLGGFASENFASLSPLEKVGDILWHSVLPLLSYMAG
                     SFAVTTFMMKNSLMDNLSADYVRTAIAKGLSFRRAVYRHAVRNSLIPIATSFGGNIAL
                     ILTGSFLIERIYNIDGFGLLGFESLVERDYPVVLGILVISSVLFLLGNILSDICVALV
                     DPRVKFG"
     gene            3982..4626
                     /locus_tag="VM692_08935"
     CDS             3982..4626
                     /locus_tag="VM692_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015166315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUQ52334.1"
                     /translation="MRAKLYVGNRNYSSWSIRGSLLVRQSGLDCDEMVIPLDHEDSVE
                     RIAAVSPSGRVPVLHADGIVVWDSLAIAEFLHERHPSAGLWPRDFAARALARSVSAEM
                     HSGFGALRSQMPMDMRARHTVPRTAELVKDIARIDALWIACRAAHGSGGEFLFGAWSA
                     ADALYAPVVSRFRTYGVELSPVARAYANAVWQWPALQALAAEAAAEPWSLGLEI"
     gene            4629..5165
                     /locus_tag="VM692_08940"
     CDS             4629..5165
                     /locus_tag="VM692_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HUQ52335.1"
                     /translation="MARHHATHAELAAGLPDVHKSPRERGSLELIVRRPKSGEREVLE
                     VAELDVEQGLVGDRWALGRRRRVNQLTLINSRLVGLVAKSRDRWALAGDQLYVDFDLS
                     LEHLPPGSRVAVGSAEIEVSPEPHMGCKLFRARYGEDAHKLVNSPDGRAWQLRGINAW
                     VVKGGRVRVGDDVRALFA"
     gene            5453..>6198
                     /locus_tag="VM692_08945"
     CDS             5453..>6198
                     /locus_tag="VM692_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017961629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52336.1"
                     /translation="MERSVDSAAKPSATEVRAQLERILASRCFLQAGRASDFLRYVVE
                     QTLAGGGQRLKGYTIGVEVFGRPPDFDAQSDALVRVEAGRLRRRLVEYYASEGASDPV
                     RIDLPRGSYSVDCNFVRPAAEPEGAQAVPAAAATAPAPVLAPWRRIALGLAALLVMAV
                     GVITWQRSELHDAELSLEVLGEPQQTEWPRIVVVPFENLGADGALDAIAASMTEEIML
                     RLDELDLFVVASQARWYGATHEKRLDAPGTT"
BASE COUNT         1101 a         1913 c         2073 g         1111 t
ORIGIN      
        1 ggtgcgcaaa ccttggctga tcggatcgaa cagatacggg tccgtcatgc gcgtgccgtg
       61 cgtctccggc aagcgcaccc accgccacgc gacctcgcgc acatacggca ccttgtagag
      121 cggaatgaag gcgccgatct cccagacgcg ctgctgaatc tcgtgcgaca agcgaattcg
      181 cgtcggcagg tcgatcgcgt cacgctgctc gatgatgatt ttgtcgagct ccgcgtcgtc
      241 cgttgccgtg atgttgttcg tctgcggaat gtgcgcgttg tccgaatggt agtgctccca
      301 gaacgccgga atatgctgcg gcgaccacgc catgaaggcg atctgatgct tcttctcgag
      361 cgtctgcttg aaggtcgccg ccgagtcttg cacttgcaga ttcagctcga cgccggcctt
      421 gcgcgcctct tcgcgcagca cgaccattcg cggcgtgagc tcgttgttgc cgtaggagat
      481 cgtgaaggag agccgctgcc ggtccttgac gcgaatgccg tccggcccgc gcatcgcgaa
      541 gccggccgct ttgaaatact cgtcggcctt cgcgagatcg aacggccgcg gcccgatgct
      601 cgtgtccgtg taatcgccgt agccttcgta atgctgcttg aggcgctcgt agtcgccgcg
      661 cagcagaccg ctcagcagca ggtcgacgtt catcgagtgg tgcaacccta agcgcacgtt
      721 acgatcgccg agcagcgggt cgtccatgtt cagccacagg ccgtacgacg gcatcggcgt
      781 ctcggtgtag tacttgtact tgccgatata gcccttgtcg aacagctcgc cctgcgcctt
      841 gtcgtgccag aaattcggaa agacgacgtt gaaggtatcg agctcgccgc gcaggaaata
      901 ctcccaggcg acgttttggt cgcgaatgac ggtgagccgg atcgtgtcga cgttgtagcg
      961 atgctcgaag tagcgctgtt tgtcgcccca ccagctcggg ttgcgcttga attcgatgta
     1021 ctggcctttc tcgatgcgtg agatctggta cgggcccgtc cccggcggga ttcgccagtt
     1081 gtagtcctcg acccacttgg cgtcgagctt gtggaactgc cgcggcgtcg gcgccaagcc
     1141 gtagaagaac aacagctcgt cgcgcggctt cgcagtcgag ccttcgatcg agatcgtgtg
     1201 gtcgtcgtgc ttgcgcacgg ccacgatgtc cttcgtgtag tgatcgttgt accaagggtc
     1261 gacgatgtgc tcggagcgca tgaactcgag cgtgaacacg tagtcgtcgg ccgtgacggg
     1321 cagcccgtcc gaccaggtcg ccgacggatc gagccggtag tagaccgtct taccgtcggg
     1381 cgcgaaggcc cattccgtcg cgagctgcgg aatcggcttg cgcgtgttcg ggtgaatgtc
     1441 cgtcagcgcg gcgtcgttgg cgcgcatgta actcgtgaac gcgtcgttcg agtccgggcc
     1501 gacgagacgc agcgtcaacg ggaacgacag cagccagtag cgatactctc cgccgcgctt
     1561 tgcctcgggc gaggcgtaga tcggatccgt gaggttggtc tcccagacaa ggccttcggg
     1621 cagtggctcc gtggggtagg tcggcagact ctcgacgtag cgcgtcgtcg tcgccacgac
     1681 cgtgccggcc tctgacgcgg cgggagattc ggatgccgca ccgctcggct cgctgccgcc
     1741 gcctccgccg cacgcggcga gcaggagcgt gaggacgccg atcgcaaaac cttgtcgtgt
     1801 tgtcatagtg aaaccttggc tcgccgagcc gttcaagcgt agtaggaaaa ctgcttcgga
     1861 tcgaacgcct cgcggatggc ttcgccggtg taggcgacca tcaacaagac gacggtcatc
     1921 gccgttacga ccgatgtgac tatccacggc gcctggaggt tcgccgttcc ctggctcagc
     1981 agctcacccc agctcggcgt cggcggcggc agcccgaagc cgagatagtc gagcgcggtc
     2041 agcgccgtca ctccggccgc gatcgagaaa gggatgaagg tcacgatgat cgagatcgtg
     2101 ttggggagga tgtgccggaa cacgatccgc gtgttcgacg cgccgagcgc gcgtgcggcc
     2161 gcgacgtact cgcgcgcctt ctccttgtag gtgaccgttc gcatgtacca cgtcatgccc
     2221 atccagccga acacgaccat cgcgatcacg agcgtccaga agttcggcac gatgaccgag
     2281 gcgacgatca tgatgacgta caggaacggc acgttggacc agatctcgat gaggcgttgg
     2341 aacagcagat cgaacgcgcc gccccaaaag cccatcgcgc agccgatcga gatgccgacg
     2401 acgtaattgc acacgagcaa gatcacggcg aacgccgtcg cgagacgaaa gccgtagaac
     2461 agccgcgcta cgatgtcgcg gcccgtaacg tcggtgccga ggtaatgcct cgtcgcaaag
     2521 ctcggcggat acggcgggaa cgcgtcggtg cgaaagtcgg tttcgtaagc gttgaacggt
     2581 acgggcggca gcagcaccca attgccgtcg ctctgtgcga acgcggcctt gagctcgcga
     2641 tagttcgtct cgtagtcgta gtcgaggccg aacgtacgcc ccggaatgaa cgcggcgtag
     2701 gtcgggaagt agaagtcgcc ttcgtacttc acgacgagcg cgcgattgtt gacgaggagc
     2761 tccgcgccga ggctgagcgc caatagaacg agcgtgatga tcagcgagta gtagccgcgc
     2821 ttgatcgagc ggaagcgctt ccactgcttg atcgtctgcg gcgccaggcg aattcccagc
     2881 atgagctagc cgaacttgac tcgcggatcg acgagcgcga cgcagatgtc ggacagtatg
     2941 ttgccgagca agaacagcac cgacgagatg acgaggatgc cgagcacgac gggataatcg
     3001 cgctcgacga gcgactcgaa gccgagcagc ccgaagccgt cgatgttgta gatgcgctcg
     3061 atcaggaacg accccgtcag tatcaaggcg atgttgccgc cgaacgacgt cgcgatcggg
     3121 atcaaactgt tgcgcacggc gtgtcggtac acggcgcggc ggaacgacag ccctttggcg
     3181 atggcagtcc gcacgtagtc ggcggacaga ttgtccatca gcgagttctt catcatgaac
     3241 gtcgtgacgg cgaagctgcc ggccatgtac gacagcagcg gcagcaccga atgccaaaga
     3301 atgtcgccga ccttttccaa cgggctcagc gaggcgaagt tctcgctggc aaacccgccg
     3361 agcgggaacc aaccgagctg cgaagcgaaa tagacgagca gtgcgatgcc gatgacgtag
     3421 ctcgggaccg catagccgaa gaacacgact ccggagctca cgttgtcgaa cagtccgtgg
     3481 tgtctgattg ccttagcgat cccgagcggg atgcagaccg cgtacgtgag cacgagcgtc
     3541 gcgaggccgt agaagatcga gaccgggaac cggtccttga tcgtgtccca gaccggctcg
     3601 ttgtagcgcg tcgaccggcc gagatcgagc ttcagcacct tgcccagcca atcgacgtag
     3661 ctgacgagaa tcggcttgtc gaagccgtaa tagacgcgca gctggttgag ttgcgcctcg
     3721 gacagggtgc tgcccgctac gctgcgttgc gcgccgccgc gctccccgct cgccatcagc
     3781 gcctggttca tcatccgctc gatggggccg ccgggaacca ctcgcgtcac ggcaaagacg
     3841 agcaacgtga tgccgatgaa agtcggaacg atcagtaaga gccggcggat gaagtaactg
     3901 gtggttccca cgagacgcgg acggtgtaat tcggccagag tatatacaat gtgtctcgag
     3961 gcagcgttca cgcttcttga gatgcgcgcc aagctctacg tcggcaatcg caattactcg
     4021 tcgtggtcga ttcgtggctc gctgctcgtg cggcagagcg ggctcgattg cgatgagatg
     4081 gtcattccgc tcgatcacga ggattcggtc gagcgcatcg cggccgtgag cccgagcggc
     4141 cgcgtgccgg tcttgcacgc ggacggcatc gtggtctggg actcgctggc gatcgctgaa
     4201 ttcctgcacg agcggcaccc aagcgccggc ctgtggccgc gcgatttcgc ggctcgcgcg
     4261 ctggcgcgca gcgtcagcgc cgagatgcac tccggtttcg gcgcattgcg ttcgcagatg
     4321 cccatggaca tgcgagcgcg gcatacggtg ccgcgcacgg cggagctcgt gaaagacatc
     4381 gcgcgcatcg atgcgctctg gatcgcgtgc cgcgctgcgc acggcagcgg cggcgagttc
     4441 ctattcggcg cgtggtccgc ggccgatgcg ctctacgcgc ccgtcgtcag ccgcttccgc
     4501 acctacggag tcgagctgtc gccggtcgcg cgcgcctacg cgaacgccgt ctggcagtgg
     4561 ccggcgttgc aggcgttggc ggccgaagcc gcggccgagc cgtggtcgct cgggctcgag
     4621 atctgacgat ggcacgacac cacgccacgc atgcagagct cgcggccgga ttgccggacg
     4681 tgcacaagtc gccccgcgag cgcggctctc tcgagctgat cgtgcggcgg ccgaagtccg
     4741 gcgaacgcga ggtgctcgaa gtcgccgagc tcgacgtcga gcaagggctc gtcggcgatc
     4801 gctgggcgct cggcaggcgg cggcgcgtca atcaactgac gctgatcaat tcgcgcctcg
     4861 tcgggttggt cgcgaagagc cgcgatcgtt gggcgctcgc aggcgatcag ctctacgtcg
     4921 acttcgacct gagtctcgag cacctgccac cggggtcgcg cgtcgcagtc ggatcggccg
     4981 agatcgaggt cagccccgag ccgcacatgg ggtgcaagtt gttccgcgcg cgctacggcg
     5041 aagacgcgca caagctcgtc aactcgcccg acggccgagc gtggcagctg cgcggcatca
     5101 atgcatgggt cgtaaagggc ggcagggtgc gcgttggcga cgacgtgcgc gccttgttcg
     5161 cttgacgctc ttcgctcgaa gagttgacgg gctggaacct gctcactacc ggcccttgct
     5221 gcgagcgtct ggtctcaaaa gcccttcgtc gggcaactaa tcgaccagtt gtgtcaagct
     5281 gttgactgtg tgaggtcagg tccagccctt aagctcgagt ctacgcctcg aggatcggaa
     5341 cgtatagcgc tcgcttgcgg tcaccgatgc gtgcgtgcgc gcgctgctga agttgaagat
     5401 ggagtagtgg ttggcgatgt cgtcggatag tcgaacgagc ggccccgaaa gcgtggagcg
     5461 ttccgtcgac agtgcagcga agccgagcgc gaccgaagtg cgcgcgcaac tcgagcgaat
     5521 tctcgccagt cgctgctttc tgcaagccgg ccgagcgtcg gattttctgc gctacgtcgt
     5581 ggagcaaacc ttggctggcg gcggccaacg cctcaagggt tacacgatcg gcgtcgaagt
     5641 tttcggtcgg cccccggatt tcgatgcgca gtccgatgcc ctcgtgcgcg tcgaggcggg
     5701 gcggctgcgc cggcgcttgg tcgagtacta cgcgagcgaa ggcgcgagcg atcccgtgcg
     5761 catcgatctg ccgcgcggca gctactccgt tgactgcaac ttcgtgcgac ccgccgccga
     5821 gccggagggc gcgcaagctg tgccggcggc cgctgcaacg gcgcccgcgc ccgttctcgc
     5881 gccgtggcgg cgcattgccc tcggccttgc ggcgctgctc gtcatggcgg tcggcgtcat
     5941 cacttggcag cggtcggagc tgcacgacgc ggagttgtcg ctcgaggttc tcggcgagcc
     6001 gcagcaaacg gaatggccgc gcatcgtcgt cgtgccgttc gagaatctcg gtgccgacgg
     6061 cgctctcgac gccatcgcgg cgagcatgac cgaagaaatc atgctgcggc tcgacgagct
     6121 ggacctgttc gtcgtcgcga gccaggcccg ttggtacggt gcgacgcacg agaagcgcct
     6181 cgatgccccg gggacgac
//
LOCUS       DATLEE010000417         2466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9405965, whole genome shotgun sequence.
ACCESSION   DATLEE010000417 DATLEE010000000
VERSION     DATLEE010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9405965"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1103)
                     /gene="plsX"
                     /locus_tag="VM692_08950"
     CDS             complement(90..1103)
                     /gene="plsX"
                     /locus_tag="VM692_08950"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HUQ52337.1"
                     /translation="MSRKVTIALDAMGGDRGPESVVPAALATLERDDSVNLTLVGLPN
                     VLEQAKRQAGDRYGSRLTVKTATEVVSMDEPPADALRRKKDSSLRVAIELVKSGEADA
                     CVSSGNTGALMATARYVLKTLPGIDRPAIISAVPGVTAPTYMLDLGANVGCTPEHLLQ
                     FALMGSVVAEDMLEIERPRVGLLNIGLEDIKGNETVREAGELLAKSGLNYVGFVEGDG
                     IFHADVDVVVSDGFVGNVALKTMEGVARLISAFLKEEFRRSLLRGVQGIIAKSALEAL
                     RGRLDPRRYNGASLVGLNGIVLKSHGGADQFAFENAVKAAIVEARKGVPVQIGKLIEN
                     RPH"
     gene            complement(1167..1358)
                     /gene="rpmF"
                     /locus_tag="VM692_08955"
     CDS             complement(1167..1358)
                     /gene="rpmF"
                     /locus_tag="VM692_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HUQ52338.1"
                     /translation="MAVQKSRKTPSRRDMRRSHDALTGPTLSVDATTGVTHRRHHVTA
                     DGFYRGKKVFEIPQETEQE"
     gene            complement(1405..1872)
                     /locus_tag="VM692_08960"
     CDS             complement(1405..1872)
                     /locus_tag="VM692_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HUQ52339.1"
                     /translation="MSGSLHAWYSLRDLESLANREVTLNGELEIDRLTRLKSLLHSGV
                     GSVRATLRFRQRGDGWLGVELDYQGSVDLVCQRCLEPFRHELAESVNVVLADADSAPS
                     PPPAGYEPFELEEGRLQPAQLVEDELIVTLPLVPKHARVEDCGSLARKLAELK"
     gene            1949..>2466
                     /locus_tag="VM692_08965"
     CDS             1949..>2466
                     /locus_tag="VM692_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HUQ52340.1"
                     /translation="MPKIILASTSRYRRELLERLRLPFTTRSPDAPEIARDGEAPPAM
                     AARLALAKARSITEPGALVIGSDQVASLDDRLLRKPGSPEVAVAQLRAAQGKVVLFHT
                     AVAVIATDTGKTLEHVDRTAVRFRRLDDAALERYVGLESPLDCAGSFKAEGLGVALFE
                     QITSEDPTALIG"
BASE COUNT          441 a          836 c          742 g          447 t
ORIGIN      
        1 gtcgttggtc tcgaccatct tctcgagatc cgcgttggtg aggattcgtt ccggtagata
       61 tttgccggtg ccgacgatac gcgagtacat cagtggggcc gattctcgat gagtttgccg
      121 atctgcacgg gcacgccttt ccgagcttcg acgatcgccg ccttgacggc gttctcgaac
      181 gcgaactgat cggcgccgcc gtgactcttc aatacgatac cgttgagccc aacgaggctc
      241 gcgccattgt agcggcgcgg gtccaatcgg ccacgcagcg cttcgagcgc cgacttcgca
      301 atgatgcctt gaaccccgcg caacaagctg cggcggaact cttctttcag gaacgcgctg
      361 atcagccgcg ccacaccttc catggtcttc aacgcgacgt tgccgacgaa cccgtcgctg
      421 acgacgacat cgacgtccgc atgaaaaatc ccgtcgccct ccacgaagcc gacgtaattg
      481 agcccgctct tggccagcaa ctccccggcc tcgcgcacgg tctcgttacc cttgatgtcc
      541 tcgagcccga tgttcagcag gccgacgcgc ggccgctcga tctcgagcat gtcctcggcg
      601 acgacagagc ccatcagtgc aaactgcagc aagtgctcgg gcgtgcagcc aacgttggcg
      661 ccgaggtcga gcatgtaggt cggcgcggtg acgccgggta cggcggatat gatggcgggc
      721 cggtcgattc ccggcagcgt cttcagcacg taccgagccg tcgccatcag cgcgcccgtg
      781 ttgcccgagc tcacgcaagc gtcggcctcg ccgcttttca cgagctcgat ggccacccgc
      841 agcgacgagt ccttcttgcg gcgcagcgcg tcggccggcg gctcgtccat cgagacgact
      901 tccgtggccg tcttgaccgt gagtcgcgaa ccgtagcgat cgccggcctg gcgcttcgcc
      961 tgctcgagca cattcggcaa cccgacgagc gtcagattga cggagtcgtc gcgctcgagc
     1021 gttgcgagcg ccgcaggtac gacggactcg ggtccacggt cgccgcccat ggcgtccaag
     1081 gcaattgtga ccttgcgact cacgcgacct taaaactcga ttgtcttgcg caactgggca
     1141 gtcgccagca acgcgggacc aagcgtctac tcttgctcgg tttcctgcgg aatctcgaag
     1201 accttcttgc cgcggtagaa accgtcggcg gtgacgtggt gacgtcgatg cgttacgccg
     1261 gtggtggcat ccaccgacaa cgtcgggccg gtcaatgcat cgtgcgaacg acgcatatca
     1321 cgccgagagg gcgtcttgcg gctcttttga actgccatga tggaatctcc tagaacaaac
     1381 cttgaccgac cggtttacgg ccggtcactt cagctcggca agctttcgcg ccaagctacc
     1441 acagtcctcg actcgggcgt gtttgggcac cagcgggagc gtgacgatga gctcgtcttc
     1501 gaccaactgt gccggctgca accgaccctc ttccaactcg aacggctcgt accctgccgg
     1561 cggcggcgac ggcgccgaat ctgcatcggc caaaacgacg ttcacgctct cggcgagctc
     1621 gtgacgaaac ggttcgaggc aacgctgaca cacgagatcc accgagccct gatagtcgag
     1681 ctctacgccc agccaaccgt caccgcgctg tcgaaaacgc agtgttgctc ttacactgcc
     1741 gacaccggaa tggagcaggc tcttgaggcg cgtcagtcga tcgatctcga gctcgccgtt
     1801 gagcgtaacc tctcgattag ccaacgattc caggtcgcgc agactgtacc aagcgtgcag
     1861 cgagcccgac ataggccgcg catcatatgt gagcaggttt ctgacgtcaa aaccgtaagt
     1921 tacgcatccc gctagccgac tcgcccccat gccgaagatc atcctcgcat cgacttcccg
     1981 ctatcgccgc gagctgctcg agcggcttcg cttgccgttc actacgcggt cacccgacgc
     2041 gcccgagatc gcccgcgacg gcgaagcccc gcccgcaatg gccgcgcgac tcgcgctcgc
     2101 caaagcgcgg agcatcactg agcccggcgc cctcgtgatc ggctccgatc aagtggcgtc
     2161 gctcgacgac cgcctgctgc gcaagccggg gtcgccggag gttgcggtcg cccagctgcg
     2221 cgcggcgcaa ggcaaggtcg tgctgttcca cacggcagtc gcggtgatcg ccacggacac
     2281 gggcaagacg ctcgagcacg tcgatcgcac ggccgtacgt tttcgccgcc tcgacgacgc
     2341 cgcgctcgaa cgttacgttg ggctcgaaag cccgctcgac tgtgccggca gcttcaaggc
     2401 cgaagggctc ggggtcgcgc tgttcgagca gatcacgtcg gaagacccga cggcgctgat
     2461 cggatt
//
LOCUS       DATLEE010000418         2151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5687983, whole genome shotgun sequence.
ACCESSION   DATLEE010000418 DATLEE010000000
VERSION     DATLEE010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5687983"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VM692_08970"
     CDS             complement(<1..752)
                     /locus_tag="VM692_08970"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HUQ52341.1"
                     /translation="METAFTRHAGAQVPLICGPMYPCSNPELVAAVSEAGGLGVVQPI
                     SLTYVHGHEFRAGLEYIRSLTAKPIGFNALIEASNKAYLDRMRRWIDVALEEGVRFFI
                     TSLGNPKWVCERVHAVGGVVYHDVTERKWAQKGIDGGVDGLIAVNSRAGGHAGGLSPE
                     ALFEQLAPLGKPIVSAGGVGTPAEFARQLHLGYAAVQLGTRFIATPECKASDAYKQAI
                     VDAGEGDIVLTERLTGVPVAVIRNAHVERLGT"
     gene            complement(756..1229)
                     /locus_tag="VM692_08975"
     CDS             complement(756..1229)
                     /locus_tag="VM692_08975"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HUQ52342.1"
                     /translation="MIATGAKLLDLWRRLARLPGGPWLFSKLFGFAVPYSGSVGPRIR
                     VLEPGHAVVEIRDRRANRNHLGSVHAIALMNLAEITSGLAMIVGLPPTVRGIVTTLSM
                     TYHKKARGTIRAVARVAVPSVTEDCDFDVMAECFDHEGALVATGRVRWRLGPVRR"
     gene            complement(1234..>2151)
                     /locus_tag="VM692_08980"
     CDS             complement(1234..>2151)
                     /locus_tag="VM692_08980"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HUQ52343.1"
                     /translation="KLPVIQRAVMAACDRLDGVADGLIENPRVCRFDPSVLVCKGADA
                     ADCLPRAQVEALQQIYEGPRNPRTQERLFVGQPPGTEAVPGGWGTWITPANPAGAIQF
                     MFGNSYYGAAVFEDRNWDFRTLDFDRDVRIGDTKAGPVLNATSPDLRSFRAAGGKIIQ
                     YHGWGDAAIPAPSSIEYYEAVRDFLAEYPDARLTGAPSPEDFYRLFLVPGMAHCAGGS
                     GPNSFGNGGGGADPRNPERDIFTALERWVEQGVAPQRLVGTGRATDDASKPLTRPLCP
                     YPQVAQYRGSGDVNDAASFACAAPASTPR"
BASE COUNT          341 a          729 c          734 g          347 t
ORIGIN      
        1 ttggtgccga gccgctcgac gtgagcgttg cgaatcacgg cgaccggcac gcccgtgagg
       61 cgctccgtca gcacgatgtc gccctcgccg gcgtctacga tcgcctgctt atacgcatcg
      121 ctggccttgc actcgggcgt cgcgatgaag cgcgtgccga gctgcacggc ggcatagccg
      181 agatgcagct gccgcgcgaa ctcggccggc gtgccgacgc cgccggcgct aacgatcggc
      241 ttgcctagag gcgcgagctg ttcgaacagc gcttcgggtg agaggccccc cgcgtgtccg
      301 ccggcgcgcg agttgaccgc gatcaggccg tcgacgccgc cgtcgatgcc tttctgcgcc
      361 cacttgcgct cggtaacgtc gtggtagacg acgccgccga ctgcatgcac gcgctcgcaa
      421 acccacttcg ggttgccgag cgacgtgatg aagaagcgca cgccttcttc gagcgcgacg
      481 tcgatccagc gtcgcatgcg gtcgagataa gccttgttgc tcgcttcgat cagcgcattg
      541 aaaccgatcg gtttcgccgt cagcgaccgg atgtactcga gcccggcacg gaactcgtgg
      601 ccgtgcacgt acgtcagcga gatcggctgc acgacgccga gtccgccggc ttcgctgacg
      661 gccgcgacga gctccgggtt ggagcacggg tacatgggcc cgcagatcag tggcacctgc
      721 gcgccggcgt ggcgcgtgaa cgccgtctcc atcgctcagc gccgcacggg gccgagccgc
      781 cagcgcacgc ggcccgtcgc gacgagcgcg ccttcgtgat cgaagcattc ggccatgacg
      841 tcgaagtcgc aatcttcggt gacgctcggc acggcgacgc gcgcgacggc gcggatcgtg
      901 ccgcgcgcct tcttgtgata cgtcatcgac agcgtcgtga cgatgccgcg caccgtcggc
      961 ggcagcccga cgatcattgc gagcccactc gtgatctcgg ccaggttcat gagcgcgatt
     1021 gcgtgcacgg agcccagatg gttgcggttc gcgcgccggt cgcggatctc gaccacggcg
     1081 tggcccggct ccagcacgcg gatgcgcggg ccgacggagc ccgagtaggg caccgcgaag
     1141 ccgaacagct tcgagaacag ccagggcccg ccgggcaacc gcgcaaggcg acgccaaagg
     1201 tcgagtagct tcgcgccggt cgcgatcatc cagtcatctt ggcgtcgacg ccggcgcggc
     1261 acaggcgaag ctcgccgcat cgttcacgtc gcccgagccg cggtattgcg ccacttgggg
     1321 ataggggcac agcggtcgag tcagcggttt ggacgcgtca tcggtggcgc gtccggtgcc
     1381 gaccaagcgt tgcggcgcga cgccttgctc gacccagcgc tcgagtgcag tgaagatgtc
     1441 gcgctcgggg ttccgcgggt ccgctccacc gcctccattg ccgaagctat ttgggccgga
     1501 gccgcccgcg caatgcgcca ttccgggcac gagaaacagc cggtagaaat cctcgggcga
     1561 tggagcgccg gtcaggcgcg catccgggta ttcagcgaga aagtcgcgga ctgcctcgta
     1621 gtactcgatg gagctcggcg cggggatcgc cgcgtcgccc cagccgtggt actgaatgat
     1681 tttgccgccc gctgcacgga acgaccgcag gtcggggctg gttgcgttga ggaccggccc
     1741 ggccttcgta tccccgatgc gtacgtcgcg atcgaagtcg agcgtccgaa aatcccaatt
     1801 acggtcctcg aataccgccg cgccgtagta gctgttgccg aacatgaatt ggatcgcacc
     1861 ggccggattc gccggcgtga tccaggtgcc ccagcctccg ggcacggctt ccgtcccggg
     1921 cggctgtccg acgaagagac gttcctgcgt gcgcgggttg cgcggcccct cataaatctg
     1981 ctggagcgcc tcgacttgag ctctgggcag acaatcggcc gcgtccgctc ccttgcagac
     2041 gagcacggac ggatcgaacc tgcagacacg cggattttcg atcaagccgt cggccacgcc
     2101 gtcgagccgg tcgcaggccg ccatgacggc gcgctggatg acggggagct t
//
LOCUS       DATLEE010000419         2783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1218127, whole genome shotgun sequence.
ACCESSION   DATLEE010000419 DATLEE010000000
VERSION     DATLEE010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218127"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VM692_08985"
     CDS             <1..1075
                     /locus_tag="VM692_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ52344.1"
                     /translation="DTWKAGINWRPVESWMFRVMRQKAARAPNVDELASPRVSALDNA
                     NLDPCSIANAANITPTLRARCLATGMSNPQVGTVEDIVAGQVNIFEGTDLANLPTIEQ
                     ADTTTVGFVWTPDIGDLSNPVFSLDYYDINIQNPIEEFTPQEILDACYVLGIASECSK
                     IVRVGGTLTLPGAGVELLTRNKVYLQAEGVELGFSFGVGVGRGDLNFSGTVNKYLTHE
                     FQSLAALPVVDCNGYFGTSCEQPRPSLRFIQRTTWDYNNLSLSAQVRHLGSVDAQPPE
                     APAVFPGFRTIDSFDYLDLYASYRLKDRYLLSFGATNVTDETPPVVGNEAGSTDFNSG
                     NTFPGAYETLGRTYTMQVNIRFE"
     gene            1140..2408
                     /locus_tag="VM692_08990"
     CDS             1140..2408
                     /locus_tag="VM692_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HUQ52345.1"
                     /translation="MSPAIGQLVEQATRLASSGRWDDAERIWLEIRTREPRHAQALFS
                     LGVHALRRGDGGAARELLVAARAEAPNDRLVLMTLCAAQRHLGDAAAEREAIEAALTL
                     DPYFLPALLAKSSWFERYGTAASAAAMFSNALKVAPSPHDWPAALRADLEHAREVVAS
                     HRDALDMHLERELGALLRGLPAASVARWREAQSVVAGKSQPYNSQSNQLHVPRLPAIP
                     FFDRTQFPFLDALEAKTAVIRAELEAALEAAREEFVPYIQYAPGQPVNQWQELNHSLR
                     WSTLHLWRNGKPVEDNLARCPETARALAALPLADIGELCPNAMFSALAPKSRIPPHNG
                     ETNARVIAHLPLIVPQGCSYRVGFDWRQWRVGETLIFDDTIEHEARNDSDELRVVLIF
                     DLWNPLLTPAERELVRRLAQAARTFGSAPA"
     gene            complement(2405..>2783)
                     /locus_tag="VM692_08995"
     CDS             complement(2405..>2783)
                     /locus_tag="VM692_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HUQ52346.1"
                     /translation="GGAAERAAFLRWLVFLVANVYPTSTYADDPSRFVEGEEPQRAFK
                     ARVSAYRDKLYAQLEQVAGAPWFLGARFSALDIYVCAMSRWRPGRAWFAANAPKLAAI
                     ATGTEQLPQLAACWRRNAVGASS"
BASE COUNT          469 a          974 c          905 g          435 t
ORIGIN      
        1 cgacacctgg aaagccggca tcaactggcg gcccgtcgag tcgtggatgt tccgcgtgat
       61 gcggcaaaaa gccgcgcgcg cgccgaacgt cgacgagttg gcgagcccgc gggtcagcgc
      121 cctcgataac gcaaacttgg atccgtgctc gatcgcaaac gcggcgaaca tcacaccgac
      181 gctgcgggcg cgttgcctgg cgaccggcat gtcgaatccg caagtcggga cggtcgagga
      241 catcgtcgcc ggtcaggtca acatcttcga aggcacggat ctcgccaatc tgccgacgat
      301 cgagcaagcc gacacgacga cggtcggctt cgtctggacg ccggacatcg gcgacttgag
      361 caacccggtg ttctcgctcg attactacga catcaacatc cagaacccga tcgaagagtt
      421 cacgccgcag gaaatcctgg acgcatgcta cgttctcggg atcgcttcgg agtgctcgaa
      481 gatcgtccgc gtcggcggca cgctgacgtt gccgggcgcc ggtgtcgagc tgctgacgcg
      541 caacaaggtg tatctgcaag ccgaaggcgt cgagctgggc ttctcgttcg gcgtcggcgt
      601 aggccgcggc gatttgaact tctccggaac ggtcaacaag tacttgacgc acgagttcca
      661 gagcctagcg gcgctgcccg tcgtcgactg caacggctac ttcggcacga gctgcgagca
      721 gccgcggccg tcgctgcgct tcattcagcg cacgacgtgg gactacaaca acctgtcgtt
      781 gtcggcccaa gtgcggcatc tcggctcggt cgatgcacag ccccccgaag cgccggcggt
      841 ctttccgggg ttccgcacaa tcgactcgtt cgattatctc gacctgtatg cgagctaccg
      901 cttgaaggac cggtacctgc tgagcttcgg cgcaacgaat gtgaccgacg agactccgcc
      961 cgtggtcggc aacgaagccg gctcgacgga cttcaattcg ggcaacacgt tccccggcgc
     1021 gtacgagacg ctcggccgca cgtatacgat gcaagtcaac attcgcttcg agtgacgctc
     1081 acgcggcgga gggttcccgc cctccgccgc cggcccgccg tcgaaaccaa ctcgtcagca
     1141 tgtctcctgc cattgggcag ctcgtcgagc aggcgacccg gctcgcaagc tcgggccgct
     1201 gggacgacgc cgagcgcatc tggctcgaga tccgcacccg cgagccgcgc cacgcgcaag
     1261 cccttttcag cctcggcgtc cacgctctga ggcgcggcga cggcggagcc gcgcgcgagc
     1321 tcctcgtcgc ggcgcgcgcg gaggcgccga acgatcggct cgtgctcatg accttgtgcg
     1381 cagcgcagcg ccacctcggc gatgccgcgg ccgagcgcga ggcgatcgaa gcagcgctga
     1441 cgctcgatcc gtatttcctg ccggcattgc tcgcgaagag cagttggttc gagcgctacg
     1501 gcaccgcagc cagcgctgcg gccatgttct cgaatgcact caaagtcgcg ccgtcgccgc
     1561 acgactggcc ggccgccctg cgcgccgacc tcgagcacgc gcgtgaggtc gtcgcgtcgc
     1621 acagggacgc gctcgacatg catctcgagc gcgagctcgg cgcactgctg cgcgggctgc
     1681 cggcggcgag cgttgcgcgc tggcgcgagg cgcaatcggt cgtcgccggc aagtcgcaac
     1741 cgtataactc gcagtcgaac cagctgcacg tgccgcgcct gccggcgatt ccgttcttcg
     1801 atcgcaccca gtttccgttc ctcgacgcgc tcgaggcgaa gaccgccgtc atccgcgccg
     1861 agctcgaggc ggcgctcgag gccgcgcgcg aggaattcgt gccatacatt caatatgcgc
     1921 cggggcaacc cgtgaaccag tggcaggagc tcaatcactc gctgcgctgg agcacgctgc
     1981 acttgtggcg caacggcaag cccgtcgagg acaatctcgc gcgctgtccc gagacggcgc
     2041 gcgcgctggc ggcgctgccc cttgccgaca tcggcgagct ctgcccgaac gcgatgttct
     2101 cggcgcttgc gccgaagtcg cgcattccgc cgcacaacgg cgaaaccaac gcgcgcgtca
     2161 tcgcgcactt gccgctgatc gtgccccagg gttgcagcta tcgcgtcggc ttcgattggc
     2221 ggcagtggcg cgtcggcgag acgctaatct tcgacgacac gatcgagcac gaggcgcgca
     2281 acgacagcga cgagctgcgc gtcgtgctga tcttcgacct gtggaacccg ctgctcacgc
     2341 ccgccgagcg cgagctcgtg cgccggctcg cccaggccgc ccgcacgttc ggctcggccc
     2401 ccgcctaaga gctagcgccg accgcgttgc gccgccagca cgccgcgagc tgcggcaatt
     2461 gttccgtgcc tgtcgcgatc gcggccagct tcggcgcatt cgccgcgaac cacgcgcgtc
     2521 cggggcgcca gcggctcatc gcgcagacat agatgtcgag cgccgagaag cgcgcgccca
     2581 agaaccacgg cgcgccggct acttgctcga gctgcgcgta gagcttgtcg cgataggcgg
     2641 agacgcgagc cttgaacgct cgctgcggct cctcgccctc gacgaagcgc gacgggtcgt
     2701 cggcgtacgt ggaggtcgga tagacgttcg ccacgagaaa cacgagccag cgcaagaatg
     2761 ccgcacgctc ggccgcgccg ccg
//
LOCUS       DATLEE010000420         6311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6197487, whole genome shotgun sequence.
ACCESSION   DATLEE010000420 DATLEE010000000
VERSION     DATLEE010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6197487"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VM692_09000"
     CDS             <1..1096
                     /locus_tag="VM692_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HUQ52347.1"
                     /translation="VEDIRRIVRQIGVNRLMDEVIARLTHACDTYDAAKACAPARSGF
                     EYQLPGIGLLEWMPVIEFGRRALIKVVGYHPANPSRRTLPTILSTALTYDVETGHMLA
                     LMDGTFMTALRTGAASAVASRLLAPRQSRVLGLVGAGAQAIAQLHGLSRVLELERVLV
                     FDADARVSASFAERAALLGLDNIDIRVARLETVVTSAHVLCTATSVDVGAGPVFEDSP
                     LLHAGLHINAVGSDFPGKVEVPKSVLRRSLVCPDFLEQALREGECQQIERGDVGPDLV
                     TLVKRATEFEGYRDRPTVFDSTGWAFEDLVAADVLLGHGRDLGCGTEFVIESMSADPR
                     NPYAFACDAASTEVSDSDHGRHRADTAASA"
     gene            1195..1881
                     /locus_tag="VM692_09005"
     CDS             1195..1881
                     /locus_tag="VM692_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HUQ52348.1"
                     /translation="MYPQVVKISLSPAQCRTSALTETLDKQETERAQRFRFDHLRKRF
                     VAAHAALRQILAAHLHCDPKRVEYEPGAFGKPRVASANGSCLCFNMSHSKDLALVAIA
                     FGRDVGIDVESVDREFDHCALARQLFSAAEIERLEGEPPASRPELFFALWTAKEAYLK
                     ARGTGLAMSLDAFEIAESKHRHSWRDVIERNGQSPRWFVRELSPQPGFIASIALEERY
                     ARLDLREWVW"
     gene            complement(1892..3436)
                     /locus_tag="VM692_09010"
     CDS             complement(1892..3436)
                     /locus_tag="VM692_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HUQ52349.1"
                     /translation="MTVNVVARRGFRDTPQCQMHELDKKMARGALWMVLLRISVRSLG
                     LISTVILARLLGTADFGLVAMATSVIALLELATAFSFDIPLIQNRNTERGHFDTAWTL
                     NITLYSGLAVLLIVLAGPAAAFYHEDRLADVIYVLAIGFLAQSFDNVGVVHFRKELNF
                     ARDFWLLFGKKVIAFAVTIPLAFLLRNYWALVAGIVVGNVFGVALTYFVHPFRPRFSL
                     GHATQMLGFSKWLLFNNGIGVLQTRAADFIVGRLAGSSALGVFSMAFEIATLPTTELV
                     SPINRVALPAYSKLAGDRVALRDSYLNLLSLIAFFALPAGFGIAAVAPSLIDLFLGDK
                     WTAAVPLVQLLGVFGAVNALTTNAGALYNGIGKPRTISLLGGRHVVVLLTGCIGLVIS
                     YGVAGIAVAYVGTAILMSPVIFFFVGNELQVGLTGIGAVLWRSLLSATAMYGFVRYLS
                     SVVTAAGIDESLSVLLIVVPAGALLYVACAVALWQLSGRPLTGESRFVTTVLGRLRSL
                     LAAKRA"
     gene            complement(3557..4948)
                     /locus_tag="VM692_09015"
     CDS             complement(3557..4948)
                     /locus_tag="VM692_09015"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HUQ52350.1"
                     /translation="MSSEHLPRVIVVAALCGLLATGAALAQPPGGFGGPRPEPEIEGI
                     PRVPTAVALPSLSAAITGPGAMFDTAPSQARGLDAEHFRYKTTEYFASGTAAGKPYTT
                     RVVVRQPADDGKFSGLVLAESMHSSGAAHMFEFTSGYLMSSGHAAVEILTTSPQQFIA
                     FNAPRYEKLKVENGQQNEIIAQVGALVRSNKGPLGSLRVRKMVMGGTSMSAGTLINYL
                     PAHMVFRTPEMGRIFDGFMPTSNGSTIREIDVPMIHMPTMHEVETNVTRRQDGDEPGK
                     QYRLFEFAAIGHVDSRDNVRLLPNPCTKPLSTFLTQAYFSVSLYHLLRWVDQGIVPPR
                     APRILLDRDESNDGSTMVLDAQGNPVGGIRSPYVDVPTAKYAPVNTAAEPVIANPSEY
                     VRVNGLQGAQIMCRLSAYQEPFSAAKLRELYGSKREYLRKFEARLDELEKQGWSLPMY
                     RDTILADVAKTEF"
     gene            complement(5051..5344)
                     /locus_tag="VM692_09020"
     CDS             complement(5051..5344)
                     /locus_tag="VM692_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HUQ52351.1"
                     /translation="MAKTIPTTLALLVLAAPCALADTLLIDGIDVDKQSVDARPKPGM
                     SMTAVESTYGAPAQRHAAVGGAAVQQPPITRWDYPSFSVYFENDRVIHAVARK"
     gene            5503..>6311
                     /locus_tag="VM692_09025"
     CDS             5503..>6311
                     /locus_tag="VM692_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52352.1"
                     /translation="MFQPLEWFIGLRYLRSRRWRALVSFRTAASLIGLALGVTALIVI
                     LSVLNGLEAETRTRLLALGAHATISAPDGFTDWRKVAERYEGVPGVAAVAPYVRIEGM
                     LAAGASLRPAIVRGILPAEESKASDLPHLVERGALDNLVPGQHRILLGQQLALNLGVE
                     TGGAVRVLIPHVTNGRVVPRLEAFTVGGVFEAGVPDHDTSLALVHLADASDLKGLNGA
                     VEGIALRFDDPLFVREFAAAQRGTLEPGWRYSDWTEEYRNLFTAMRIEKLM"
BASE COUNT         1159 a         1984 c         2052 g         1116 t
ORIGIN      
        1 cgtcgaggac attcggcgga tcgtcaggca gatcggcgtc aatcggctca tggacgaggt
       61 catcgctcgt ctgacgcacg cgtgcgacac gtacgatgcg gccaaggctt gcgccccggc
      121 gcgcagcgga ttcgagtacc aacttcccgg catcggtctg ctcgaatgga tgcccgtgat
      181 cgaattcgga cgccgtgcgc tgatcaaggt cgttggctac catccggcga atccgtcccg
      241 gcgaacgctt ccgacgatcc tgtcgactgc actgacatac gacgtcgaga ccggccacat
      301 gttggcactc atggacggaa cgttcatgac tgcccttcgc acgggcgcag cgtcggctgt
      361 cgccagccga ctactcgcgc cgcgtcagtc tcgcgtgctg ggattggtcg gcgccggcgc
      421 gcaggcgatc gcgcagctgc acgggttatc gcgcgtcctg gagctcgagc gcgttcttgt
      481 cttcgacgcc gacgcgcgtg ttagcgcaag cttcgccgag cgcgccgcgc tgctcgggct
      541 cgacaacatc gacattcgcg tcgcccgtct ggaaacggtc gtgacctccg cgcacgtcct
      601 ttgcacggca acctcggtcg acgtcggcgc tggcccggtt ttcgaggaca gccctctgct
      661 tcatgcagga ctgcacatca atgccgttgg ctcggatttt ccgggcaagg tcgaggtgcc
      721 gaaaagcgtc ttgaggcgta gcttggtttg ccccgatttt ctcgagcaag cgctcagaga
      781 gggcgaatgc cagcagattg aacgtgggga cgtcgggccg gacttggtga cgctcgtcaa
      841 gcgcgcgaca gagttcgaag gctatcgcga ccgaccgacc gtcttcgatt ccacggggtg
      901 ggccttcgag gacctcgtag ccgccgacgt actgctcggt cacggacgcg acctcggctg
      961 cggcaccgag ttcgtcatcg agagtatgtc cgcggatccg aggaatccct acgcgttcgc
     1021 ctgcgatgcg gcctcaacgg aagtatcgga cagcgaccac ggacgccatc gagcggacac
     1081 ggcagcctcg gcatgaaggc atcggagccc gttcttcggt cgcgctgagg gatcgtcgtg
     1141 gaggagacag cttgtcattg gctcccaatg ccggacggcg ggtcacgaca cggcatgtat
     1201 ccgcaagtcg taaagatcag cttgtcgcca gcgcaatgcc gcacgtcagc gctgacagaa
     1261 acgctggaca agcaagagac ggagcgtgcg cagcgcttcc gtttcgatca tctacgaaag
     1321 cggttcgtag ccgcacacgc ggcactgcgc caaatcctcg ctgcccacct ccattgcgac
     1381 ccaaaacgcg tcgaatacga accgggcgct tttggcaagc cgcgtgtcgc atccgctaat
     1441 ggctcgtgtc tctgcttcaa catgtcgcat tccaaagacc ttgcactcgt tgccatcgcg
     1501 ttcggccgcg acgtaggtat cgacgtcgag tctgtggacc gcgagttcga ccattgcgca
     1561 ctcgctcgcc agcttttttc agctgcggag atcgagcgct tggaaggcga gccgccggcg
     1621 agtcggccgg agctcttttt cgcgctctgg accgccaagg aagcgtacct aaaagcgcgc
     1681 ggtaccggct tggcgatgtc gctcgatgcg ttcgagatcg ctgagtcgaa gcatcggcac
     1741 tcatggcgag acgtcatcga gcgaaacggc cagagccctc gctggttcgt acgcgagctg
     1801 tcacctcagc cgggattcat cgcatcgatc gcgctggagg agcgatacgc tcgactcgat
     1861 ttgcgggagt gggtttggtg aaggcaccgc gctaggcgcg ttttgccgcg agcaacgatc
     1921 gtagtcggcc gagcacagtg gtgacaaatc gcgactctcc agtcagcggt cgccccgaga
     1981 gctgccagag tgcaacggcg cacgctacgt agagcaaggc accggccgga acgacgatca
     2041 acagcacaga cagcgactca tcgatgccgg ccgcggtcac tacagatgaa agatagcgga
     2101 cgaagccgta catcgccgtg gccgacagca gagaccgcca cagcacggca ccgattcctg
     2161 tcaatccgac ctgtagctcg ttgccgacga aaaaaaagat gaccggtgac atcagtatcg
     2221 ccgtgccgac gtatgcaact gcaatcccag cgacgccgta agagatgacc agcccgatgc
     2281 atcctgtcag caacacaacg acgtgccttc cgcccagcaa tgaaatcgtc cgcggcttgc
     2341 cgattccgtt gtacagcgct ccggcgttcg ttgtgagcgc gttgacggcg ccaaacacgc
     2401 ccagtagctg aacgagcggc actgccgccg tccatttgtc gccgagaaac aaatcgatta
     2461 acgacggcgc cacggccgcg atcccgaacc ccgccggcaa ggcgaagaac gcgatcagtg
     2521 acagtagatt gaggtagctg tcgcgcagcg ccacgcgatc gcccgcaagc ttcgaatacg
     2581 ccggcagtgc cacccggttg atcggggaca cgagctccgt cgtgggcagc gtcgcaatct
     2641 cgaaggccat cgagaacacg cccagggcgg acgaacctgc aagcctgccg acgatgaagt
     2701 cggcggcccg cgtctgcaaa acaccgatac cgttgttgaa caggagccat ttcgagaagc
     2761 ccagcatctg tgtcgcgtga cccaaggaga accgcggccg gaacgggtgc acgaaatacg
     2821 tgagagcgac gccgaagacg tttcccacca ctattcctgc gacgagggcc cagtagtttc
     2881 gcagcaagaa cgccaacggg atggtgacgg cgaacgcgat taccttcttc ccgaacagca
     2941 gccaaaaatc tctggcgaaa ttcagttcct ttctgaagtg aacgacaccg acgttgtcga
     3001 agctttgagc gaggaagccg atcgcgagga cgtagatgac gtcggcgagg cggtcttcgt
     3061 gatagaaggc tgcagccggg ccggctagca cgatgaggag cacggcaagg cccgaataca
     3121 acgtgatgtt cagcgtccag gcggtgtcga aatgcccgcg ctccgtgttc cgattttgga
     3181 tgagcggaat gtcgaagcta aacgccgtcg cgagctcgag tagcgcgatc accgaggtcg
     3241 ccatcgcgac gagcccaaaa tcggcggtac ccaacaggcg cgccagaatg acggtgctga
     3301 tcaagccgag gctgcgtact gagatgcgga ggagcaccat ccacagtgct ccgcgagcca
     3361 tctttttatc gagctcatgc atttggcatt gcggagtatc acgaaatcca cgacgagcga
     3421 ccacgttgac cgtcacgacg gctcagcggc gtgattcgtc acggaggagg aagctgctgc
     3481 gtcgagctcg actacttcta gtcgcgccgc ctgatcaaca agtacgatgt cgtgaacggg
     3541 gcggtcggcg ccagcgctaa aactccgttt tcgcgacgtc ggccagaatc gtgtcgcggt
     3601 acatcggcag cgaccagcct tgcttctcga gctcgtcgag gcgcgcctcg aacttgcgca
     3661 agtactcgcg cttgctgccg tagagctcgc gcagcttcgc cgccgagaac ggctcttggt
     3721 acgcgctcaa gcggcacatg atctgcgcgc cttgcagtcc gttgacgcgc acgtactcgc
     3781 tgggattcgc gatgacgggc tcggcggccg tgttgacggg cgcgtatttc gccgtcggca
     3841 cgtcgacgta cgggctacgg atgccgccga cgggattgcc ttgcgcgtcg agcaccatcg
     3901 tagaaccgtc gttgctctcg tcgcggtcga gcaggatgcg tggcgcgcgc ggcggcacga
     3961 tgccttggtc gacccaacgc agcaagtgat acagggacac cgagaagtac gcctgcgtca
     4021 ggaacgtcga gagcggcttc gtgcagggat tcggtagcag gcgcacgttg tcgcgcgagt
     4081 cgacgtggcc gatcgctgcg aactcgaaca accggtattg cttgcccggc tcgtcgccgt
     4141 cttggcggcg cgttacgtta gtctcgacct cgtgcatcgt cggcatgtgg atcatcggca
     4201 cgtcgatctc gcggatcgtc gagccgttcg atgtcggcat gaagccgtcg aagatgcggc
     4261 ccatctccgg cgtgcggaac accatgtggg cgggcaaata gttgatcagc gtcccggcgc
     4321 tcatcgacgt tccgcccatg accatcttgc gcacgcgcag cgacccgagt ggacccttgt
     4381 tgctgcgaac gagcgcgccg acttgggcga tgatctcgtt ctgctggccg ttctcgactt
     4441 tgagcttctc gtagcgcggc gcattgaacg cgatgaattg ctgcggcgac gtcgtcagga
     4501 tctcgacggc cgcatggccc gagctcatga ggtagcccga cgtgaactcg aacatgtgag
     4561 cggcgccgct cgagtgcatc gactccgcga gcacgagccc gctgaacttg ccgtcgtcgg
     4621 cgggctggcg cacgacgacg cgcgtcgtat aaggcttgcc tgcggcggtt cccgacgcga
     4681 agtactcggt cgtcttgtag cggaagtgct cggcgtcgag cccgcgcgct tgcgacggcg
     4741 ccgtgtcgaa catcgcgcct gggccggtga tggccgccga cagcgacggc agcgcaacag
     4801 cggtgggaac tcgcgggata ccttcgatct cgggctcggg acgcggcccg ccgaaaccgc
     4861 ccggcggctg cgcaagggct gcgcccgtgg cgagcaagcc gcacaacgcg gcgacgacga
     4921 tgactcgcgg caagtgctct gacgacatgg tgttcccccc aacgagggca caccttaccg
     4981 cacggtgcgc gcaatacaga aggcccggcg ggttcggctc gccgggctca aggcacgtga
     5041 agcgggagta ttacttgcgg gcgacggcgt ggatgacgcg atcgttctcg aaatacaccg
     5101 agaacgaggg gtagtcccac cgcgtgatcg gcggttgctg cacggcggcg ccgccgacgg
     5161 cggcatgacg ctgcgccgga gcgccgtagg tcgactcgac ggccgtcatc gacatgccgg
     5221 gcttgggccg ggcgtcgacg ctctgcttgt ccacgtcgat accgtcgatc agcagcgtgt
     5281 cggcgagcgc gcacggggct gcaagcacca gcaacgccaa cgtcgtcgga atggttttgg
     5341 ccatggccag atcctccctg gaatggccga aatataacac cgcccaaaag gccctcaaat
     5401 tcgtggaagg ccttttgagg cgccggtcac acttcgcttg caggccgcgt cactgccggc
     5461 tccgcgtccg gggcccaagt cgttatggtt taatcgcagc tcatgtttca accgctcgag
     5521 tggttcatcg gcctgcgtta cctgcgctcg cggcgttggc gtgcgctcgt gtcgttccgc
     5581 accgcggcat cgttgattgg cctcgcgttg ggcgtcacgg cgctgatcgt catcctgtcg
     5641 gtgctgaacg ggctcgaggc ggagacgcgc acgcgcctgc tcgcgctggg ggcgcacgcg
     5701 acgatctcgg cgcccgacgg cttcacggat tggcgaaagg tcgccgagcg gtatgagggc
     5761 gtgccgggtg tcgcggccgt cgcgccgtac gtgcgcatcg agggcatgtt ggccgccggc
     5821 gctagcctgc ggcccgcgat cgtgcgcggc atcctgccgg ccgaggagag caaggcgtcg
     5881 gatctgccgc atctcgtcga gcgcggggcg ctcgacaatc tcgtgccggg tcagcaccgg
     5941 atcctgctcg gtcaacagct ggcgctgaac ctcggcgtcg agaccggcgg cgccgtgcgc
     6001 gtgctgatcc cgcatgtgac gaacggccgc gtcgtgccgc gcttggaggc ctttaccgtc
     6061 ggcggcgtgt tcgaggccgg cgtgccggac cacgacacgt cgctcgcgct cgtgcatctc
     6121 gccgatgcca gcgacctcaa aggattgaac ggcgccgtcg aaggcatcgc gctgcggttc
     6181 gacgacccgc tgttcgtgcg cgagttcgcc gcggcgcaac gcggcacgct ggagcccggt
     6241 tggcgctact ccgattggac ggaggagtat cgcaacttgt tcactgcgat gcgcatcgag
     6301 aagctcatga t
//
LOCUS       DATLEE010000421         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9412799, whole genome shotgun sequence.
ACCESSION   DATLEE010000421 DATLEE010000000
VERSION     DATLEE010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9412799"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VM692_09030"
     CDS             complement(<1..314)
                     /locus_tag="VM692_09030"
                     /inference="COORDINATES: protein motif:HMM:NF023792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3653 domain-containing protein"
                     /protein_id="HUQ52353.1"
                     /translation="MFVTDEWIVARACVHPATVRRWRARGEFPPALQRLAELEHYGEL
                     GHIDERWREWRIVAGELVAPNGERYTPGVILALALRLQQLNELERLARAPRRGPLGVF
                     DRL"
     gene            566..>2290
                     /locus_tag="VM692_09035"
     CDS             566..>2290
                     /locus_tag="VM692_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUQ52354.1"
                     /translation="MTFGEQRVLVDADFAIESGERVCLIGRNGAGKSTTLKLIGGSQE
                     PDDGAVEKPARLRWSMLEQKLADESPEIVRDFVARGMADQLQRIAEFQRLTAMGSEDR
                     AALREIEALEREVEAGGGWNIDVHIATVISNLGLPGELRMSELSGGWRRRAALAQALV
                     SNPELLLLDEPTNHLDISTIEWLEATVRSYPGAVLFVTHDRSFVERVATRIVDIDRGK
                     LRSWPGAYNNFLAQKAQAEQDEDRQNALFDKKLADEEIWVRKGVKARQTRNEGRVRAL
                     EELREIRAARIKRPRSARVQINEAAEVSGRKVIELRNVTHGYGGTTLLKDFSLRVMRG
                     DRLGIIGNNGVGKSTLLRILLGELTPDSGSVKLGTNLEVGYFDQLRRELDPSKTVAEI
                     VGEGREYVTIHGREKHVVAYLTDFLFPAKRARTPIAALSGGERNRVILAKLFTRAANL
                     LVLDEPTNDLDVETLEALEERLAEYDGTLLVVSHDRYFLDAIVTSTLVFEADGEIRRH
                     AGGYSDWLERHRELAVTDEPTAPAGRVSRDTREKPAQRKLSYKLQRELDALPEQIERL
                     EQRVVELRA"
BASE COUNT          432 a          740 c          740 g          378 t
ORIGIN      
        1 agccgatcga acacgccgag cgggccgcgc cgcggcgcac gcgcgaggcg ctcgagctcg
       61 ttcaattgtt gcaatctgag tgcaagcgcg agaatcacgc ccggcgtgta gcgctcgcca
      121 ttcggtgcga cgagctcgcc ggcgacgatc cgccactcgc gccagcgctc atcaatgtgc
      181 ccgagctctc cgtagtgctc gagctcggcg agccgttgca gcgccggcgg aaattctccg
      241 cgcgcgcgcc aacgtcgaac ggtcgccggg tgcacgcacg cacgcgcgac gatccactcg
      301 tcagtgacaa acatggaaag aaactccctt cgtgttgttc ttatttgcgg gatgcaacta
      361 tttagccagc ggcggcgtgc gctcgcgcgc cggcgccgac gcggcgcgga tcgcgtcggt
      421 tcgcataatg tatattatgt tagatacagg aggcagcgac gaatgagacg gtttgtgctg
      481 ccgcccgtcg cggctacagt aaaccctccc gttcaaaccc ccgtgcttgg ccatggttcc
      541 tttggtgcgt ttcgacgccg tttcggtgac attcggcgaa cagcgagtcc tcgtcgacgc
      601 cgacttcgca atcgaatccg gcgagcgcgt ctgtttgatc ggccgcaacg gggccggcaa
      661 gtcgacgacg ctgaagctga tcggaggctc ccaggagccc gacgacggcg cggtcgagaa
      721 acccgcgcgc ctccgctgga gcatgctcga gcaaaaactt gccgacgagt ccccggagat
      781 cgttcgagac ttcgttgcgc gcggcatggc cgaccagctg caacggatcg cggagtttca
      841 gcgcctgacg gccatgggtt ccgaggaccg cgccgccttg cgcgagatcg aagcattgga
      901 gcgcgaggtc gaagccggcg gcggttggaa catcgacgtc cacattgcga ccgtgatcag
      961 caatctcggt ttgccgggcg agctacgcat gagcgagttg tcgggcggtt ggcggcgccg
     1021 cgccgctctg gctcaagcgc tggtatcgaa ccccgagctg ctgctgctcg atgagcctac
     1081 caatcatctt gacatcagta ctatcgaatg gctcgaggcg accgtacgat cgtatcccgg
     1141 tgccgttctg ttcgtgactc acgaccgtag cttcgtcgag cgagtcgcta cacgcatcgt
     1201 cgacatcgat cgcggcaagc tgcgcagctg gcccggcgcc tacaacaact tcctggcgca
     1261 aaaagcccag gccgagcagg acgaggatcg gcagaacgcc ctcttcgaca agaagctcgc
     1321 cgacgaagag atctgggtgc gcaaaggcgt caaggcgcgg caaacacgca acgaaggacg
     1381 tgtccgggcc ctcgaggagc tgcgcgagat tcgtgccgca cgcatcaagc ggccgcggtc
     1441 ggcgcgcgtg cagatcaacg aggcggccga ggtatccggc cgcaaggtca tcgagcttcg
     1501 caacgtcacg cacggctatg gcggcacgac tctattgaag gacttctcgt tgcgtgtcat
     1561 gcgcggcgac cgactgggca tcatcggcaa caatggcgtc ggcaagagca cgttgctgcg
     1621 catactgctc ggcgagctga cacccgactc gggatccgtg aagctcggca ccaatctcga
     1681 ggtcggctac ttcgatcagc tgcgccgcga gctcgacccc agcaagaccg tcgccgagat
     1741 cgtcggtgaa ggccgcgagt acgtcacgat tcacggccgc gagaagcacg tcgtcgccta
     1801 tctgacggac tttctctttc ccgcgaaacg ggccaggacg ccgatcgcgg cgttatcggg
     1861 cggcgaacgc aaccgcgtca tcctcgccaa gctgtttacg cgcgcggcga atctactcgt
     1921 gctcgacgag ccgaccaacg acctcgacgt cgagacgctc gaggcgctcg aggagcgcct
     1981 cgccgaatac gacggcacat tgctcgtcgt gagccatgat cggtatttcc tcgacgcgat
     2041 cgtcacgagc acgctcgtgt tcgaggccga cggcgaaata cgccggcacg ccggcggcta
     2101 cagcgactgg ctcgagcgac atcgcgagct tgctgtcacg gacgagccga cggcgccggc
     2161 gggccgcgtc tcgcgagaca cgcgcgagaa gccggcgcag cgcaagctgt cgtacaagct
     2221 gcagcgcgag ctcgacgcgc tgcccgagca aatcgagcga ttggaacaga gggtcgtcga
     2281 gctgcgcgcc
//
LOCUS       DATLEE010000422         8615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_478167, whole genome shotgun sequence.
ACCESSION   DATLEE010000422 DATLEE010000000
VERSION     DATLEE010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478167"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VM692_09040"
     rRNA            complement(<1..804)
                     /locus_tag="VM692_09040"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1230..2666
                     /locus_tag="VM692_09045"
     CDS             1230..2666
                     /locus_tag="VM692_09045"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUQ52355.1"
                     /translation="MKRQTSGVRHDYKDTFVASSSRGRWALFAFGVSLPIIGSVLLLL
                     GTPRQPLAVRPTETLLEPPAPSAAIAPLPAGPIDLGSDVNLDAAPAEAVEPPGIILDL
                     LVKRGDTLEVLFRRNGLSLTDLAAMVALPDASAALKLLKPGDRLEISHRDGQVLSLRR
                     ELDDIKLLSIARQESGFAANTIERAVDIRTTAAHGEIRSSLFEAGADAGISDHTTMDM
                     AGIFEWDIDFIQDPREGDTFTVIYEELWRDGVKLRDGEIVAAEFVNQGKSFRAARFRD
                     ESGRAGYFTPEGRSVRKAFIRAPLNFSRISSNFNPSRRHPVLNTIRAHRGVDYAAPTG
                     TPVRAAGDGKVLFRGVQGGYGNTIILQHGGNITTLYGHLSRFGNARTGGRVNQGDVIG
                     YVGSSGLATGPHLHYEYRVNGAHRNPRTVSLPPADPIPAEQQLAFETATEPLWRQLDG
                     YLRSAGTTVQAEEPAADVEPGSAPVSPN"
     gene            2761..4836
                     /gene="ppk1"
                     /locus_tag="VM692_09050"
     CDS             2761..4836
                     /gene="ppk1"
                     /locus_tag="VM692_09050"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HUQ52356.1"
                     /translation="MDALNLKLPDYYINRELSQLAFNVRVLKQAMDVGVPLLERLKFL
                     CISSTNLDEFFEIRVSGLKHRLAVNAAPAGPDNLSSADVLRTVTEEANALVTEQYRVL
                     NDELIPKLREAGIRFVRRDDWSPAQRVWLEQYFRTEIVPLLSPTTLDPTRPFPRILNK
                     SLNFIVSLDGRDAFGRPCHRAIVQAPRTLPRLIPLPPTLEGTGKTDFVFLSSVIHAFV
                     DQLFAGMEIDGCYQFRVTRNSDLFIDDEDVDDLMTALEGELFESRYGAAMRLETAHDC
                     PEELAAYLLEHFRLTRADLYRVPGPVNLNRLLAVYDLVDRPELKYGPFTPGLPKSLVK
                     TNIFEAIAKQDILLHHPFESFAPVIDFIYRAATDPDVLAIKQTLYRTTPDSPLVESLM
                     SAAKAGKEVTVLIELRARFDEAANIELASKLQEAGAHVVYGVVGYKTHCKMSLVVRRE
                     HGKLRRYVHLGTGNYHPRTARAYTDYGLLSANETLGEDVHQVFMQLTSLMRTPDLNLL
                     SQSPFNLHERLLGLIRRETRNAASGGTGHIIAKLNALVEPEVIRALYEASGAGVYVDL
                     IVRGICCLRPGIPGVSENIRVRSIVGRFLEHSRVYYFHNGGNEELYCASADWMERNFF
                     RRIELMFPIADAPLKARVMTDLDTYLADSVQAWELRKDGHYEQSRPSPDRDATPAQLK
                     LLRQLAEAS"
     gene            complement(4833..6365)
                     /gene="ppx"
                     /locus_tag="VM692_09055"
     CDS             complement(4833..6365)
                     /gene="ppx"
                     /locus_tag="VM692_09055"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HUQ52357.1"
                     /translation="MVVPDPQARLVETAEVMAAVDLGSNSFHMVVARNQHGEPSIVDR
                     LREMVRLASGLDDKGYLDAASQERALACLRRFGQRLRDMRAHQVRVVGTNTLRRAENS
                     DAFLAKAEEALGHPVEVISGIEEARLIYLGVSHHTDSTDGANLVIDIGGGSTELIIGE
                     GYEPQHLESLYVGCVGVSRAHFDDGKLSAKRFERARLAVRLELRPVAAAFRRRGWKRV
                     LGSSGTIRTARDIAQELKLAEGGVSIAAVDAIIEAMIKARRVEDLQLLGLGPERAPVF
                     AGGIAILAEVMSTLKIERLDISTGALREGLLYDMLGRLHDEDARERSIRAMQRRYHVD
                     LEQAARVEATAVALLDQVANDWQLAQQRYRQLLVWAARLHEVGLDIAHARYHHHGGYL
                     IANSDLPGFVRLEQQLVASLVTLHRRKLDDPFLDDLPDSWRPPMLKLVVLLRLATLLH
                     RSRSPSDLPQIELRPGAKSLALEFPRGWLDANPLTAADLEQEEGWLKTRGFDLEIEQP
                     RD"
     gene            complement(6384..6740)
                     /gene="erpA"
                     /locus_tag="VM692_09060"
     CDS             complement(6384..6740)
                     /gene="erpA"
                     /locus_tag="VM692_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HUQ52358.1"
                     /translation="MTDTTPLAEAPPLNFTDAAAHKVGQLIEQEGNAALKLRVYVQGG
                     GCSGFQYGFTFDEEVQEGDTEIENAGVTLLVDPMSVQYLMGAEIDYREDLSGAQFIIR
                     NPNATTTCGCGSSFSV"
     gene            complement(6744..7169)
                     /locus_tag="VM692_09065"
     CDS             complement(6744..7169)
                     /locus_tag="VM692_09065"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HUQ52359.1"
                     /translation="MLGKKSRSQAPIGTLVGAETRVHGDIEFSGGFHVDGYVKGNVRA
                     TKDERSVLSISERGCVEGSVVVAHVVLNGTVKGDVRATERVELGPKARVIGNVQYRLI
                     EMSIGAEVNGKLIHESEHAAAKSATRPQVDATRPLNAGE"
     gene            complement(7187..7891)
                     /locus_tag="VM692_09070"
     CDS             complement(7187..7891)
                     /locus_tag="VM692_09070"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HUQ52360.1"
                     /translation="MRRRTGRPTWMYALVALLGAGSLYLAFELGRYQSGFSILDQRHE
                     REQLDGRLADERGVSDELRRQLAIAQTSEEIDRATYSQVEATLGELQAKIQAQEEELV
                     FYRGIVSPQDGVAGLRIQSLEVLPSEAEGEGRYVLRLLLVQAIVHSRRVSGAVKLQLE
                     GMQDGQTVSFDAAELVAPDEPYDMAYEFRYFQGLETELALPQGFEPLHVAVEIWPNEA
                     KAERISQTFEWPTESD"
     gene            complement(7893..8447)
                     /locus_tag="VM692_09075"
     CDS             complement(7893..8447)
                     /locus_tag="VM692_09075"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HUQ52361.1"
                     /translation="MDFIDEQGFRANVGIILTDGGGRVLIAGRRGRGGWQFPQGGIQP
                     EESSEAAMFRELREEVGLAPTDVELVGSTADWLRYRLPDRYIRREQTPLCIGQKQRWF
                     LLQLTTDSSRVRFDLSPEPEFDRYRWVDYWRPVKEVVYFKRPVYVRALHELGPLAFPN
                     DGPPPPPAWWPRRWQSALSERAPS"
BASE COUNT         1544 a         2876 c         2570 g         1625 t
ORIGIN      
        1 ggcgtggact accagggtat ctaatcctgt ttgctcccca cgctgtcgca cctgagtgtc
       61 agtattgggc caggtagccg ccttcgccac tgatgttcct cccgatatct acgcatttca
      121 ccgctacacc gggaattcca ctaccctctc ccatactcta gctgagtagt atcgactgca
      181 attcccaggt taagcccagg gctttcacaa tcgactgact cagccaccag cgtgcgcttt
      241 acgcccagta attccgatta acgcttgcac ccaccgtatt accccggctg ctagcacgga
      301 gttagccggt gcttcttcta agggtaacgt caagctcgga tgtgttagat ccgatttttt
      361 cttcccctct gaaagtgctt tacaacccgc aggccttctt cacacacgcg gcattgctgg
      421 atcagggttg cccccattgt ccaatattcc ccactgctgc ctcccgtagg agtctgggcc
      481 gtgtctcagt cccagtgtgg ctgatcgtcc tctcagacca gctacggatc gtcgccttgg
      541 taggccgtta ccctaccaac tagctaatcc gacgtaggct catcctttag cgcgaggtct
      601 tgcgatcccc cgctttcctc cgtagagcgt atgcggtatt agcccggctt tcgccgggtt
      661 atcccccact acagggcaga ttcctacgta ttactcaccc gtccgccact cgtcgccagg
      721 gttgcccccg cgttaccgtt cgacttgcat gtgttaggca tgccgccagc gttcaatctg
      781 agccaggatc aaactcttca cttaattact caaaaagaca taaagactgt gagctttttc
      841 gagcctaaac caatgggtgc aagcacccgc tggcgtaggc tctggcacta ttgctcttgc
      901 gagcgcccac acaagttgct tgagacagat tgttaaagag cgatgcctca ccggacgcgg
      961 caggcagact tggaatttta gctccagtct cgcgcgcctt ccaagccctc tttcgaggag
     1021 tgggattcgg aaaggcaggg gcgcgcatta taagcgttga cccgatcgat acaagccccg
     1081 ggtgaaactt ttttcgcgcc gtgcgatgga ttccatcgac gcgatttaag actcggtcgc
     1141 cgcggccgag ttccgcagcg agacctcagt gttttgacca ccctattctc ggccgctaca
     1201 gtacggtgcg ttctccggac tggtgttggg tgaaacggca aaccagtggc gtccgccacg
     1261 actacaaaga caccttcgtc gcttcgtcca gccgcggacg ctgggcgctg ttcgcattcg
     1321 gcgtgtcgct acccatcatc ggttcagtac tactgctgct cggcacgccg cgccagccgc
     1381 tagccgtccg gcccacggaa actttgctcg agcctcccgc cccttcggcg gccatcgcac
     1441 cgttgccggc aggccctata gatctgggct cggacgtcaa cctcgacgca gcgcccgcgg
     1501 aagccgtcga gccgccaggc atcattctcg acttgctcgt gaagcgcggc gatacgctcg
     1561 aggtcttgtt ccgccgcaat ggactgagcc tcaccgacct cgccgcgatg gtcgcgttgc
     1621 ctgacgccag cgccgcgctg aagctgctga agcccggcga ccggctcgag atctcgcatc
     1681 gcgacggcca agtcctgtcg ctgcgacgcg agctcgacga catcaagctg ctgtcgatcg
     1741 cgcggcagga atcgggcttc gccgcgaaca cgatcgagcg tgcagtcgac atccgcacga
     1801 ccgcagcgca tggcgagatc cgcagctcgc tgttcgaagc cggcgcggat gcaggcatca
     1861 gcgatcacac cacgatggac atggcgggca ttttcgaatg ggacatcgac ttcatccagg
     1921 acccccgcga aggtgacacg ttcaccgtga tctacgagga gttgtggcgc gacggtgtga
     1981 agctgcgtga cggcgagatc gttgcggccg aattcgtcaa tcaaggcaaa tcctttcgtg
     2041 ctgcgcgctt tcgcgacgag tcgggacgcg ccggctattt cacaccggaa gggcgaagcg
     2101 tgcgcaaggc attcattcgc gcgccactga acttctcgcg tatcagttcg aacttcaacc
     2161 cgagccgccg tcatcccgtg ctgaacacga ttcgcgcgca tcgcggagtc gattacgccg
     2221 cgccgaccgg cacaccggtt cgcgctgccg gcgatggcaa ggtgctgttc cgcggcgtcc
     2281 aaggcggcta cggcaacacg atcatcctgc agcacggcgg caacatcacg acgctctacg
     2341 gtcacctatc gcgcttcggc aacgcgcgca ccggcggccg cgtgaatcaa ggcgacgtca
     2401 tcggctatgt cggaagcagc ggcctcgcga cggggccaca cctgcactac gagtatcgcg
     2461 tcaatggcgc gcatcgcaat ccgcgcacgg tatcgttgcc gccggccgat ccaattccgg
     2521 ccgaacagca actcgcattt gaaaccgcga ccgagccgct gtggcggcag ctcgacggct
     2581 atctgcggag cgccggcacg acggtgcaag cggaagagcc ggccgccgac gtcgagccgg
     2641 gaagcgctcc cgtaagcccg aactgatcag tgtgacgcga gcccggtctc gcgccgaatc
     2701 aagcgtcgta tactgggttc acggcgcgtt acccttctga aacattcgcg tcgattgcac
     2761 gtggacgcgc tgaatctcaa gcttccggac tactacatca accgtgagtt gtcgcagctc
     2821 gcgttcaacg tgcgcgtgct gaagcaagcg atggacgtcg gtgtcccgct gctcgagcgc
     2881 ttgaagttcc tttgcatctc gtccacgaac ctcgacgagt ttttcgagat tcgagtgtcg
     2941 ggattgaagc atcgactcgc cgtgaatgcg gcgccggcgg ggcccgacaa tttgagctcg
     3001 gccgacgtgt tgcgcaccgt cacggaagaa gccaacgcgc tcgtgacgga gcaatatcgg
     3061 gttctcaacg acgagttgat tccgaagctg cgcgaggctg ggatccgctt cgtgcgccgc
     3121 gacgattggt cgcccgcaca acgcgtttgg ctcgagcagt actttcgcac ggagatcgtg
     3181 ccgctgctga gcccgaccac gctcgatccc actcgaccgt tcccacgcat actgaacaag
     3241 agcctgaatt tcatcgtgag cttggacggg cgcgacgctt tcggccggcc gtgccatcgc
     3301 gccattgtgc aggcgccgcg cacgctgcca cgactcattc cgttaccgcc gacgctcgag
     3361 ggtactggca agacggattt cgtctttctt tcctcggtca tacacgcctt cgtcgaccag
     3421 ctgttcgccg gcatggagat cgacggctgt taccagttcc gcgtcacgcg caacagcgat
     3481 ctcttcatcg acgacgaaga cgtcgacgac ttgatgaccg cgctcgaagg cgagctgttc
     3541 gagagccgtt acggcgccgc gatgcgcctc gagaccgctc acgattgccc cgaggagctc
     3601 gcggcgtatt tgctcgagca cttccggctc acgcgcgcgg atctctatcg ggtccccggc
     3661 cccgtcaact tgaatcgtct cctcgccgtc tacgacttgg tcgatcgacc ggagctcaag
     3721 tacgggccgt ttacgccggg cttaccgaag tcgctcgtga agaccaacat cttcgaggcg
     3781 atcgcgaaac aagacatctt gctgcaccat ccgttcgagt cgttcgcgcc cgtcatcgat
     3841 ttcatttatc gcgcggccac ggatcccgac gtactcgcga tcaagcagac actgtacaga
     3901 accacgcccg actcgccgct cgtcgaaagc ttgatgtccg cggcgaaggc cggcaaggaa
     3961 gtgaccgtgc tcatcgagct gcgcgcgcgc ttcgacgagg cggccaacat cgagctcgcg
     4021 agcaagttgc aggaggccgg cgcgcacgtg gtctacggcg tcgtcggcta caagacacac
     4081 tgcaagatgt cgctcgtcgt gcgccgcgag cacggcaagc tgcggcgcta cgtccacctc
     4141 ggaaccggca actatcatcc gcgcacggcc cgggcgtaca cggactatgg gctcctatcg
     4201 gctaacgaaa cgctcggcga ggacgtgcac caagtgttca tgcagctgac gagcctcatg
     4261 cgcacgccgg acctgaacct gctgtcgcag tcgccgttca atctgcatga gcggctgctc
     4321 ggactgatcc gccgcgagac ccgcaatgcc gcaagcggcg gaacgggtca catcatcgcc
     4381 aagctcaacg cgctcgtgga gcccgaggtc atccgggctc tctacgaggc gtcgggtgca
     4441 ggcgtctacg tcgacttgat cgtgcgcgga atttgctgct tgcggcccgg gatccccgga
     4501 gtatcggaaa acatcagggt ccgctcaatc gtgggccggt tcttggagca cagccgcgtc
     4561 tattactttc acaacggcgg caacgaggag ctctactgcg cgagcgccga ttggatggag
     4621 cgcaactttt tccggcgaat cgagctgatg ttcccgatcg cggacgcgcc gctcaaggca
     4681 cgtgtcatga cggacctcga cacctatctc gccgacagcg tccaagcatg ggagctgcgc
     4741 aaagacggcc actacgagca gtcgcgcccg agccccgacc gcgacgccac accggcacag
     4801 ttgaagctgc tgcggcagct cgccgaagcg tcctaatcgc gcggttgctc gatctcgagg
     4861 tcgaagccgc gcgtcttgag ccaaccctct tcttgctcga gatcggcggc ggtcaagggg
     4921 ttcgcgtcga gccagcctcg cgggaactcg agcgcgagcg atttcgcgcc tggccgtagc
     4981 tcgatctgcg gcaaatccga agggctacgg ctgcgatgca gcagcgtggc cagccgcaac
     5041 aacaccacga gcttcagcat cggcggccgc cacgagtccg gcaggtcatc gaggaacgga
     5101 tcgtcgagct tgcggcggtg cagcgtgacg aggctcgcga ccaattgctg ctcgaggcga
     5161 acgaagcccg gcaaatcgga gttcgcgatc aaatagccgc cgtgatggtg gtaacgggcg
     5221 tgcgcgatgt cgagcccgac ttcgtggagc cgagcagccc acaccagcag ttgtcggtag
     5281 cgttgctgcg caagctgcca gtcgttcgcc acctgatcca gcaacgcgac cgcggtcgct
     5341 tcgacgcggg ccgcctgctc gagatcgacg tgatagcgac gttgcattgc acgaatggag
     5401 cgctcgcgcg cgtcctcgtc gtgcagccga cccaacatgt cgtacagcaa gccctcgcgc
     5461 agcgcacccg tgctgatatc gagccgctcg atcttcaagg tgctcatgac ctcggcgagg
     5521 attgcgatcc cgccggcaaa caccggcgcg cgttcggggc cgagccccaa aagctgcaga
     5581 tcctcgacgc gccgcgcctt gatcattgcc tcgatgatcg catcgacggc cgcgatgctg
     5641 acgccgcctt ccgcgagctt gagctcctgc gcgatgtcac gcgcggtccg gatggtgccc
     5701 gacgagccga gcacgcgctt ccagccgcgg cgccgaaacg cggccgcgac gggccgcaac
     5761 tccaggcgga cggcgaggcg cgcccgctcg aaccgcttcg cgctgagctt cccgtcgtcg
     5821 aagtgcgcgc ggctcacgcc cacgcagccg acgtacagac tctcgaggtg ctgcggctcg
     5881 tagccctctc caatgatcag ctcggtgctg ccgccgccga tgtcgatgac gaggttcgcg
     5941 ccatccgtcg agtcggtgtg gtgcgacaca ccgaggtaga tgagtcgcgc ctcttcgata
     6001 ccggagatga cctcgacggg atgcccgagc gcctcttcgg ccttcgcgag aaatgcgtcg
     6061 ctgttttcgg ctcgccgcag cgtattcgtg ccgacgacgc gaacctggtg agcgcgcatg
     6121 tcgcgcagcc gctgaccgaa gcgccgcagg caggcgagag cacgctcctg actcgcggcg
     6181 tcgagatacc ctttatcgtc caggccggac gcgagccgga ccatctctcg caacctgtcg
     6241 acgatcgacg gctcgccgtg ctggttgcgc gcgacgacca tgtggaagct gttcgagcct
     6301 aggtcgacgg ccgccatgac ctcggcggtt tcgacgagac gcgcctgcgg gtcgggcacc
     6361 accacggccc gtagcgcttt gagctagacg gagaacgacg agccgcaacc gcaggtcgtc
     6421 gttgcgttcg gattgcgaat gatgaattgc gcgccggaaa ggtcctcgcg atagtcgatc
     6481 tcggcaccca tgaggtattg aacgctcatc ggatcgacga gcaacgtgac gcccgcgttc
     6541 tcgatctccg tgtcgccttc ctgcacttct tcgtcgaacg tgaagccgta ttggaagccc
     6601 gagcagccgc cgccttgcac gtagacgcgc aacttcaagg ccgcgttacc ctcttgctcg
     6661 atcagctgcc cgaccttgtg ggccgccgca tcggtgaaat tcagcggcgg cgcttcggcc
     6721 agcggggttg tgtctgtcat cggctactct ccggcgttga gcggccgcgt cgcatcgacc
     6781 tgcggacgcg tcgcggactt cgccgctgca tgctcgctct cgtgaatcag cttaccgtta
     6841 acttccgcgc cgatcgacat ttcgatgagc ctgtattgca cattgccgat gacgcgcgcc
     6901 ttcggcccga gctccacgcg ctccgtcgcg cgcacgtcgc ctttgacggt accgttgagc
     6961 acgacatgcg ccacgacgac ggagccttcg acgcaaccgc gctcgctgat gctgagcacg
     7021 gagcgttcgt ccttggtcgc gcggacgttg cccttgacgt agccgtcgac gtgaaagccg
     7081 ccggaaaact cgatgtcgcc gtggacgcgg gtctctgcgc cgaccaaggt gccgatgggc
     7141 gcctgactgc gcgatttctt acctagcatg tacgctccgg tgtcgttcag tccgactcag
     7201 tgggccactc gaacgtttga ctgatgcgct cggctttggc ctcgttcggc cagatctcga
     7261 ccgcgacgtg cagcggctcg aagccctgcg gcaaggctaa ttccgtctcc aggccctgaa
     7321 aatagcgaaa ttcgtaagcc atatcatagg gctcgtcggg cgccacgagc tcggccgcat
     7381 cgaacgagac cgtttgaccg tcttgcatgc cttcgagttg cagcttgacg gcgccggaca
     7441 cgcgccggct gtgcacgata gcttgaacca gcaacagccg cagcacataa cggccctcgc
     7501 cctcggcctc gctcggcagc acttcgaggc tctgaatgcg cagccccgcg acgccgtcct
     7561 gcggcgagac gatgccgcga taaaaaacga gctcctcttc ctgcgcctgg atcttcgctt
     7621 gcagctcgcc gagcgtcgcc tcgacctgcg agtacgtggc ccggtcgatc tcctcggacg
     7681 tttgcgcgat cgcgagctgc cggcgcagct cgtcgctgac gccgcgctcg tcggccaagc
     7741 gcccatcgag ttgctcccgc tcgtgccgtt gatcgagtat cgagaagccg ctctgatacc
     7801 ggccgagctc gaacgcaagg tacaggctgc ccgcgccgag caacgcgacg agcgcataca
     7861 tccacgtcgg tctgcccgtg cgacgtcgca tgctagctcg gcgctcgctc gctcaaggcg
     7921 ctctgccagc gccgcggcca ccatgccggc ggcggcggcg gtccgtcgtt cggaaatgcg
     7981 agcggcccta gctcatgcag ggcccgcacg tagactggcc gcttgaaata cacgacttcc
     8041 ttgaccgggc gccagtaatc gacccagcgg taacgatcga actccggctc agggctcaaa
     8101 tcgaatcgca cgcggctgct atcggtcgtc aactgcaaca agaaccagcg ttgcttctgt
     8161 ccgatgcaca gcggcgtctg ctcgcggcgg atgtagcggt ccggcaaccg atagcgcagc
     8221 caatccgccg tcgagccgac gagctcgacg tcggtcggcg caagccctac ttcctcgcgc
     8281 aactcgcgga acatggcggc ctcgctgctt tcctcgggct gaatgccgcc ttgcgggaat
     8341 tgccaaccgc cgcgcccgcg tctgccggcg atcaatacgc ggccgcctcc gtcggtcaga
     8401 atgatgccga cgttggcgcg gaagccctgt tcgtcgatga aatccatgtt cgcgccgatt
     8461 ctttcatatc cacccaaccg gttccaaagc gaggcttgcc aaggtgtgac tcacttagcg
     8521 attagccgcc ctcgcgacgg catttcgccc gcctgccttc gcctcataga gcgccgcgtc
     8581 ggccagggca agcaatcgtt cgccgacggc gttca
//
LOCUS       DATLEE010000423         2332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5692857, whole genome shotgun sequence.
ACCESSION   DATLEE010000423 DATLEE010000000
VERSION     DATLEE010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5692857"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VM692_09080"
     CDS             <1..427
                     /locus_tag="VM692_09080"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HUQ52362.1"
                     /translation="GITQSLGSALSIEAERDALVARLRAFTPLVRGTSASHIARYIRA
                     DDAFHRIFLSLLRNQPLFDIYNAMDLPEIMRRVLVVAPISIREIFDDHKALTNALRAG
                     SADETSAAITQHANRVRTALAAFLAESQKAERASSQQVA"
     gene            519..785
                     /locus_tag="VM692_09085"
     CDS             519..785
                     /locus_tag="VM692_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52363.1"
                     /translation="MSNYVKLATDAGDQYLSSLAEAQENFLKATTAFATRFAPATPIE
                     TPPAFATAFPTVQEVTEASFAFAQKLLKQQKSFAEKLLASSVTK"
     gene            930..1289
                     /locus_tag="VM692_09090"
     CDS             930..1289
                     /locus_tag="VM692_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52364.1"
                     /translation="MADDKPNNDAPLIERWLTEQQQWQRTFMTYLDSMVKNDDFLVHL
                     GNAMRGSLLAGKAYPTPPGAAAAAPETPGDDKLDQILFALRQLEGQVQDLRLTLDELR
                     AAKKPLTKKTAPPKASQ"
     gene            1286..>2332
                     /locus_tag="VM692_09095"
     CDS             1286..>2332
                     /locus_tag="VM692_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUQ52365.1"
                     /translation="MNEAEPEHERDQERELEALIELSRRGRAKLERLYELLTTPGHYG
                     TGATPAAVVYRENKLRLLRLLDETGKPREGKPPVLFVPAPVSRYFIMDLLPGRSFAGY
                     VAAANFDVYIADFGTPSREDRFADLSYYVEGLVRRCVRAVSALTGHDAINVVGYCLGG
                     TLALLYTALHPETVRRLVLLTTSVDGAVEGGIPWVASKMGLEGESYDNPRLVPAAEVK
                     SWFEMLAPGSNSVVGRVSDLWDRLDDAPERLREVRTMASWVDDVVPAPGRLLAELYRK
                     LGPGANGLMKGTVAIGDRLVDLASVAMPVLSVSAEKDTIAPAAGVDAIGAIVPHAEIL
                     RLPGGHVGIVAGRAA"
BASE COUNT          412 a          772 c          758 g          390 t
ORIGIN      
        1 cggcatcacg cagtcgctcg ggtcggcgtt gtcgatcgag gccgagcgcg acgcgctcgt
       61 cgcgcggttg cgcgcgttca cgccgctcgt gcgcggcacg tcggcgtcgc acatcgcgcg
      121 ctacattcga gccgacgatg cgttccaccg aatcttcctg agcttgctac gcaatcagcc
      181 gctgttcgac atctataacg cgatggactt gccggagatc atgcgccgcg ttctcgtcgt
      241 cgccccgatc tcgatccgcg agatcttcga cgaccataaa gcgctgacca acgcgctgcg
      301 cgcgggcagc gccgacgaga cgagcgccgc catcacgcag cacgccaacc gcgtccgcac
      361 ggctctggcg gctttcttgg ccgaatctca gaaggccgaa cgagcgtcca gccagcaagt
      421 cgcgtagttc actacgcaga cgcttgacat gcgctgcaga tgcgttgcat actacgcacg
      481 tgttaaaaca aagcccgcga agaccaggag ctaccgagat gtcgaattat gtgaagttgg
      541 cgacggacgc cggcgaccag tatctttcct cgcttgccga agcccaagag aacttcttga
      601 aggccacgac ggcatttgcc acgcgcttcg cgccggcgac cccgatcgag accccgcccg
      661 ccttcgccac ggcgttcccg acggtgcagg aagtcacgga ggcgagcttc gccttcgcgc
      721 agaagctgct gaaacagcag aagagcttcg ccgagaagct gctggccagc tcggtcacga
      781 agtaactcga atcacgcgcc gcggcggcgg cctttctccc ccctaggccg tcgccgcgtt
      841 tttgccgcgt gtgagtcgac ttcagtacca taccgagcga agggactcgc ttaggtctac
      901 agttgcgacg cgagatgcct tggacttgcg tggctgacga caaacccaac aacgacgcgc
      961 cgctgatcga acgctggctg accgagcagc agcagtggca acgaacgttc atgacctatc
     1021 tggattcgat ggtcaagaac gacgatttcc tcgttcattt aggtaacgcg atgcgcggct
     1081 cgctgctggc cggcaaagcg tacccaaccc ctccgggcgc cgcagcggct gctcccgaaa
     1141 cgccgggcga cgacaagctc gaccaaatac tgttcgcttt gcgccagctc gagggacagg
     1201 ttcaagacct gcgcctgacg ctcgacgagc tacgcgccgc aaagaaaccg ctcaccaaga
     1261 agacagcgcc gcccaaggcg tcgcagtgaa cgaagcggag cccgagcacg agcgcgatca
     1321 ggagcgcgag ctcgaggccc tgatcgaact gtcgcgccgc ggccgcgcca agctcgaacg
     1381 tctctatgag ctgctgacga cgcccggcca ctacggcacg ggtgcgacac cagcggccgt
     1441 cgtctaccgc gagaacaagc tgcgcttgct gcggctgctc gacgagaccg gcaagccgcg
     1501 cgaaggcaag ccgcccgtgc tgttcgtgcc ggcgccggtc agccgctact tcatcatgga
     1561 tcttctgccg ggccgctcgt tcgccggcta cgtggccgcg gcgaacttcg acgtctatat
     1621 cgccgacttc ggcaccccga gccgcgagga tcgctttgcg gatctctcgt actacgtcga
     1681 agggctcgtg cggcgctgcg tgcgtgcggt cagcgcgctg acgggccacg acgcgatcaa
     1741 cgtcgtcggc tattgcctgg gcggcacgtt ggcgttgttg tacacggcgc tgcatcccga
     1801 gaccgtgcgc cggctcgtgt tgttgacgac gagcgtcgac ggcgccgtcg aaggcggcat
     1861 tccgtgggtc gccagcaaga tgggcctcga gggcgagagc tacgacaatc cgcggctcgt
     1921 gccggccgcc gaggtcaaga gctggttcga aatgttggcc ccggggtcga acagcgtggt
     1981 cggccgggtc agcgacctct gggaccggct cgacgatgct cccgagcggc tgcgcgaagt
     2041 ccgcacgatg gcgagctggg tcgacgacgt cgtgccggcg ccgggccgcc ttctcgccga
     2101 gctctatcgc aagctcggcc cgggtgccaa tggtttgatg aagggcacag ttgccatcgg
     2161 cgaccggttg gtagacttgg cgtcggtggc gatgcccgtg ctatcggtgt ccgccgagaa
     2221 ggacacgatt gcgcccgcgg ccggagtcga tgcgatcggc gcgatcgtgc cgcacgccga
     2281 gattctgcgg ctgccgggcg ggcatgtcgg gatcgtggcc ggccgtgcgg cc
//
LOCUS       DATLEE010000424         5536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6200351, whole genome shotgun sequence.
ACCESSION   DATLEE010000424 DATLEE010000000
VERSION     DATLEE010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6200351"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VM692_09100"
     CDS             <1..1151
                     /locus_tag="VM692_09100"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HUQ52366.1"
                     /translation="QTLIGVTGTNGKTTVAYLLAQVLSQPQRPCAYVGTLGYGVPPAI
                     TSHGLTTPDTLTLHRELTELDAPRVAMEVSSHALKQDRVAGLTFNTAVFTNLTRDHLD
                     EHGDLASYGDAKRKLFKIPGLKAAVLNCDDPFAATIAADLAPGCTLIRTSLRGSAVEL
                     WGRLKRSDLGGLELDVAGQFGAGRLSSKLIGAFNAENLLSTLGALVAQDMPLSAACAA
                     LGVAKPAPGRMEVLGGPPDRPWVVIDYAHTPDALQRVLTTLEAAVLGELWCVFGCGGD
                     RDRGKRSLMGAVAADLADRIVLTDDNPRSEDPAEIVREIRAGVGDHPRVSVVHDRRTA
                     LKTAVERARPGDVVLIAGKGHEDEQLVGNERRAFSDRAVVAELLGVAP"
     gene            1151..2509
                     /gene="murF"
                     /locus_tag="VM692_09105"
     CDS             1151..2509
                     /gene="murF"
                     /locus_tag="VM692_09105"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HUQ52367.1"
                     /translation="MARTLAAVAREVQGTLVGADQAFGLVTTDTRALGPGSLFVAIPG
                     DRFDGNDFVDQAAAKGAAGALVSRFAAASALPQIEVRDSRRALGAMARAWRASFSIPV
                     VAVTGSSGKTTVKELVAAILSVSRSICVTQGNLNNEIGVPLTLMRLTAEHDALVIELG
                     ANHAGEIDYLARLAQPTVGIITNAAEAHLEGFGSLRGVCEAKGELLDHLPRAGTAVLN
                     ADDQFRKDWVARSRCEFTVTFGYAANADCRVLGEPHFLATGSEFRMHLPDGEEADVWL
                     PLLGRQNVANALAAAAAAQAVGASTEDIVAGLARAAPVRGRLRAVPGRNGATIIDDSY
                     NANPGSVHAALDHLAALSGRRVLVFGNMAELGPTGPALHKEIGEYARGRCDLLFAIGE
                     LAGEAAKAFGAAGRSLPDIEAARSALDPLLASDTTVLVKGSRVMGLDRLVRTLEIETR
                     AD"
     gene            2510..3598
                     /gene="mraY"
                     /locus_tag="VM692_09110"
     CDS             2510..3598
                     /gene="mraY"
                     /locus_tag="VM692_09110"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HUQ52368.1"
                     /translation="MLMYLTEYLKELYSGFNVFSYLTTRAILGALTALVLSFAFGPYL
                     IQKLQMQRIGQQIRQVGPESHFPKAGTPTMGGALILLAIAISTLLWANLSNRFVLLVL
                     GGTLFFGVIGFVDDYKKVILRNSKGLSARAKIFWQTVGALGLAIAFYYTANNPSIEHS
                     LLIPYVPNVSIPLGAVGYILFVTLVIVSASNGVNLTDGLDGLAIMPSVLVGSALGIFA
                     LAAGNVIYSNYLGIPYVAGSGELLVFCAALAGAGLGFLWFNTYPAQVFMGDIGALALG
                     AALGLVAVAVRQELVLFIMGGVFVVESVSVIIQVASYKMTGRRIFRMAPLHHHFELKG
                     WPEPKVIVRFWIITVILVLIGLASLKIR"
     gene            3676..5013
                     /gene="murD"
                     /locus_tag="VM692_09115"
     CDS             3676..5013
                     /gene="murD"
                     /locus_tag="VM692_09115"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HUQ52369.1"
                     /translation="MANAAVKHSLIVGLGATGVAVARYLTAHGERVRVIDSRAAPPGL
                     AELRAAVPNIDVSLETLDERWLEDASRVLLSPGLPYDLPLAVEARRRGIAVIGDIELF
                     ALAVRAPVIAVTGSNGKSTVTTLASKILESQRLRAPAGGNLGPPALDLLSAAEADAYV
                     LEISSFQMETTDSLAPLAATVLNVSADHLDRHGTLELYAELKEKLLKSAEQAVVNVDD
                     PLVAAMGARHARVVAFSVQQELKDGYSIVTRGGERWLARDRKPVLRSAELGIRGTHNE
                     ANALAALALTAELTTDMEAALGVLRTFKGLPHRCQVVAERKGVTFVDDSKGTNVGATL
                     AALNGLAGPLVLIAGGLSKGQDLLPLAAGARGKLRGAVLIGAAADELERALGPIAKTV
                     RATSMPDAVARAANLARRGDTVLLSPACASQDMFRDYRDRGEQFARAAQELPE"
     gene            5019..>5536
                     /locus_tag="VM692_09120"
     CDS             5019..>5536
                     /locus_tag="VM692_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HUQ52370.1"
                     /translation="MVKRPVEVSKGGPNAVAFDAALVCAVVGLIAMGTIMVTSASVSI
                     VADDPLFYLVRHLGAVAIGVAGLALVVLLPTELWYRLSWLLLVVGLGLLVIVLVPAIG
                     HSVNGSRRWLELGPITVQASEPARLCLLLYLASYAVRRSTELGASLNGLVKPLFVVAA
                     AAVLLLAEPDFGA"
BASE COUNT          823 a         1858 c         1887 g          968 t
ORIGIN      
        1 cgcaaacgct catcggcgtc accggaacga acggcaagac gacggtggcg tacttgctcg
       61 cgcaagtgct gagccagccg cagcgcccgt gcgcgtatgt cggcacgctc ggttacgggg
      121 tgccgcccgc catcaccagc cacgggctga ccacacccga caccctgacg ctgcaccgtg
      181 agcttactga gctcgatgcg ccgcgcgtcg cgatggaggt gtcgtcgcac gcgctcaagc
      241 aagaccgcgt cgcgggcctg accttcaata ccgccgtgtt caccaatctc acgcgcgatc
      301 acctcgacga gcatggcgat ctcgcgagct acggcgacgc gaagcgcaag ctattcaaga
      361 tcccggggct caaagcggcc gtgctgaatt gtgacgatcc gttcgcggcg acgatcgccg
      421 cggatctcgc gccaggctgc acgttgatcc gcacgagcct gcgcggctcc gccgtcgagc
      481 tctggggaag gctcaagcgc tccgacctgg gcgggctcga gctcgacgtc gcgggccagt
      541 tcggtgccgg tcgactgagc tcgaagctga tcggcgcctt caacgccgag aacctgttgt
      601 cgacgctcgg cgcgctcgtc gcccaagaca tgccattgtc ggcggcttgt gcggcgctcg
      661 gtgtggcgaa gcccgcgccg ggccgtatgg aggttctcgg cggcccgccc gacaggccgt
      721 gggtcgtcat cgattacgcg catacgcccg acgctttgca acgcgtgctg acgacgctcg
      781 aggccgcagt gctcggcgag ctctggtgcg ttttcggctg cggcggtgat cgcgatcgcg
      841 gcaagcgctc gctgatgggt gccgtcgcgg ccgacctcgc ggatcgcatc gtgttaacag
      901 atgacaatcc gcgctctgaa gacccggccg agatcgtccg cgagattcgc gccggcgtcg
      961 gcgatcatcc gcgcgtcagc gttgttcatg atcggcgcac ggcgcttaag accgccgtcg
     1021 agcgcgcacg gcccggcgat gtcgttctga ttgccggcaa aggtcacgaa gacgagcagc
     1081 tcgtcggcaa cgagcgccgc gcgttcagcg accgtgccgt cgttgccgag ctcttggggg
     1141 tagcgccgtg atggcacgca cgctggccgc cgtcgcgcgc gaggtgcagg gcaccctcgt
     1201 cggcgccgac caagccttcg gcctcgtgac gacggacacg cgcgcgctcg ggcccggctc
     1261 gctgttcgtt gcgattccgg gcgatcgctt cgacggcaac gacttcgtcg atcaagccgc
     1321 agcgaaaggc gccgcgggcg ctttggtatc gcggttcgcg gcggcgtcgg cgttgccgca
     1381 gatcgaagtg cgcgactcgc gccgcgcgtt gggcgccatg gctcgcgctt ggcgcgcctc
     1441 gttctcgatc ccggtcgtcg cggtcaccgg cagcagcggc aagacgacgg tcaaggagct
     1501 cgtcgcggca atcttgagcg tcagccgctc gatctgcgtc acgcaaggca acttgaacaa
     1561 tgagatcggc gtgccgctga cgttgatgcg gctgacggcg gagcacgacg cgctcgtcat
     1621 cgagcttggc gcgaaccacg ctggcgagat cgactacctc gcgcggctcg cgcaaccgac
     1681 ggtcggcatc attacgaacg cggcggaagc gcacttggaa ggcttcggct cgctgcgcgg
     1741 agtctgcgag gccaaggggg agctgctcga tcacttgccg cgcgcgggca ccgcggtgtt
     1801 gaacgccgac gatcaattcc gcaaggactg ggtcgcgcgg tcgcgctgcg agttcacggt
     1861 gacgttcggc tatgccgcga acgccgactg ccgcgtgctc ggcgagccgc acttcctcgc
     1921 gacgggctcg gagttccgca tgcacctgcc cgacggcgag gaagccgacg tgtggctgcc
     1981 gctgctcggc cgccaaaacg ttgcgaacgc gctcgccgcc gccgcggcgg cgcaagccgt
     2041 cggcgcaagc accgaggaca tcgtcgccgg cctcgcgcgc gcggcgccgg tccgcggccg
     2101 gctgcgcgcc gtgccgggcc gcaacggcgc cacgatcatc gacgacagct acaacgcgaa
     2161 tcccggctcg gtgcatgccg cgctggatca tctcgcggcg ttgagcggcc gccgggttct
     2221 cgtgttcggc aacatggccg agctcggccc gacggggccg gccctgcaca aagaaatcgg
     2281 cgagtacgcg cgcgggcgct gcgacctcct gttcgcgatc ggcgagctcg ccggcgaagc
     2341 ggccaaggct ttcggcgccg cgggccgctc gcttcccgac atcgaggccg ctcgttccgc
     2401 gctcgatccc ttgctcgcga gcgacacgac ggtgctcgtc aaaggctcgc gcgtcatggg
     2461 cctcgatcgg ttggttcgca ccctcgagat cgagacgagg gctgactaga tgctgatgta
     2521 cctcaccgag tacctcaaag agctctactc gggcttcaac gtcttcagct atctgacgac
     2581 gcgcgcgatc ttgggcgcgt tgacggcgct cgtgttgtcg ttcgcgttcg ggccgtacct
     2641 gattcagaag ctgcagatgc agcgcatcgg ccagcaaatt cgccaggtcg gtcccgaaag
     2701 ccactttccg aaggccggca cgccgacgat gggcggcgcg ttgatcctgc tcgcgatcgc
     2761 gatcagcacg ctgctctggg ccaacttgag caaccgcttc gtgctgctcg tcctcggcgg
     2821 cacgttgttc ttcggcgtca tcggcttcgt cgacgactac aagaaagtca ttctgcgcaa
     2881 ctcgaagggg ctcagcgcgc gagcgaaaat cttttggcag accgtcggcg cgctgggtct
     2941 cgcgatcgcg ttctattaca cggcgaacaa cccgtcaatc gagcattcgc tgctgattcc
     3001 atacgtcccg aacgtcagca ttccgctcgg ggccgtgggc tacatcttgt tcgtcacgct
     3061 cgtgatcgtc agcgcgagca acggcgtgaa cctcacggac ggtctcgacg gcctcgcgat
     3121 catgccgtcg gtgctcgtcg gatcggcgct cggcatcttc gcgctcgcgg cgggcaacgt
     3181 catctattcg aactatctcg ggatcccgta cgtcgcgggc tccggcgagc tattggtttt
     3241 ctgtgcggcc ctagctggcg ccggcttggg cttcttgtgg ttcaacacct atcccgcgca
     3301 agtcttcatg ggcgacatcg gtgcgttggc actcggtgcc gctctgggac ttgtcgcggt
     3361 tgcggtgcgg caagaactgg tgctgttcat catgggcgga gtcttcgtcg tcgaatccgt
     3421 ctcagtcatc atccaggttg cctcctacaa gatgaccggc cggcgcattt ttcgcatggc
     3481 gccgctacac catcacttcg agctcaaggg ttggcccgag cccaaagtga tcgtgcgctt
     3541 ttggatcatt accgtgattt tggtgttgat cggcttggcg agcttgaaga tccgttgatg
     3601 ttggcgactg cgctcacgct gacgagcttc cgtcgacgag agcgcggcgc gggcctagaa
     3661 caaaaggaac gggcggtggc gaacgccgcg gtaaagcaca gtctcatcgt cggcctcggc
     3721 gcgaccggcg tggccgtcgc gcgctatttg accgcgcacg gcgagcgcgt gcgcgtcatc
     3781 gacagccgtg cggctccgcc gggtcttgcc gagctgcgcg cggcggttcc gaacatcgac
     3841 gtgtcgctcg agacgctcga cgagcgttgg ctcgaggacg cgagccgcgt gctgctctcg
     3901 ccggggctgc cgtacgactt gccgctcgcc gtcgaggctc gccgccgcgg tatcgccgtc
     3961 atcggcgaca tcgagctgtt cgcgcttgcg gtgcgggcgc ccgtcatcgc cgtgacgggc
     4021 agcaacggca agagcaccgt gacgacgttg gcatcgaaga ttctcgaatc gcagcggttg
     4081 cgcgcgccgg ccggcggcaa cttggggccg ccggcgctcg acctcttgag cgcagcggaa
     4141 gccgacgcgt acgtgctcga gatctcgagc ttccagatgg agacgaccga cagcttggcg
     4201 ccgctcgcgg cgaccgtgct caacgtctct gcggatcacc tcgatcgcca cggcacgctc
     4261 gagctgtatg ccgagctcaa agagaagctg ttgaagtcgg ccgagcaagc cgtcgtcaac
     4321 gtcgacgatc cgctcgtcgc cgccatgggc gcgcgccacg cgcgtgtcgt cgccttctcg
     4381 gtccaacaag agctcaaaga cggctactcg atcgtcacgc gcggcggcga gcgttggctc
     4441 gcccgcgatc gcaagccggt gctgcgctcg gcggagctcg gaattcgcgg cactcacaac
     4501 gaggcgaacg cgcttgcagc gctcgcgctg acggccgagc tgaccacgga catggaggcg
     4561 gcgctcggcg tgctgcggac gttcaagggt ctgccgcatc gctgccaggt cgtcgccgag
     4621 cgcaagggcg tgacgttcgt cgacgactcg aaaggcacga acgtcggcgc cacgctggca
     4681 gcgctgaacg ggctcgccgg accgctggtt ctgatcgccg gcggcttgag caagggacag
     4741 gatttgctgc cgctcgcggc cggtgcgcgc ggcaagctgc gcggcgccgt gctgatcgga
     4801 gcggcggccg acgagctcga gcgtgcgctc gggcccatag caaagacggt gcgcgcgacg
     4861 agcatgcccg acgccgtcgc acgcgcggcc aacctcgcgc ggcgcggcga caccgtgctg
     4921 ctgtcgccgg cgtgcgcgag ccaagacatg ttccgcgact accgcgaccg cggcgagcag
     4981 ttcgcgcgcg cggcgcagga gctgcccgag tgagcgccgt ggtaaaacgc ccggtggaag
     5041 tctccaaagg ggggccgaac gccgtcgctt tcgatgctgc gctcgtctgc gccgtcgtcg
     5101 gcctcatcgc gatgggcacg atcatggtga cgtcggcctc cgtgtcgatc gtcgccgacg
     5161 acccgctgtt ctacctcgtg cggcatctcg gcgccgtggc catcggcgtc gccggtctcg
     5221 cgctcgtcgt gctgctgccg acggagcttt ggtatcgctt gagctggctg ctgctcgtcg
     5281 tgggattggg cttgctggtc atcgtgctgg tgccggccat cggccactct gtcaacggca
     5341 gccgccgctg gctcgagctc gggccgatca cggtgcaagc atcggagccc gcgcgcctct
     5401 gcctgctgct ctatctcgcg agctacgccg tgcgtcgctc gacggagctc ggcgccagcc
     5461 tgaacgggct cgtgaagccg ttgtttgtcg tcgccgcggc cgcggtattg ctgctggccg
     5521 agcccgactt cggcgc
//
LOCUS       DATLEE010000425         2046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1226580, whole genome shotgun sequence.
ACCESSION   DATLEE010000425 DATLEE010000000
VERSION     DATLEE010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226580"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="VM692_09125"
     CDS             complement(<1..1822)
                     /locus_tag="VM692_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ52371.1"
                     /translation="MQLLVEAECARIKGARIEAADLYDRAIAAARAQGYINVEALAAE
                     LAARFWLRDGKPEFARIYLDKALQGYEAWGATGKVADLVARHGLGAASGSTVSVTSSV
                     TTPGPAAERSDALDLATLLKASQAIAGEIVLERLLTKLMDIIRENAGAESVVLVLETN
                     GEFLVQGVKTAAAQARVLGGEALRLTAACATGIVNYVLRTSELVVLDDAAQQGKFRGD
                     VYVVNRRPKSVLCAPVAHKGKLIGAVYLENNQVAGAFTPDRLEALNILMSQVAVSIDN
                     ATLYSRQEQQSRTIEAANVTLTKEIAERNRAEGELSRYKDHLEDLVKERTRELENAQG
                     RLVDLSRRAGMAEVASGVLHNVGNVMNSVNVGASVAREAVTALPVEGVSRAVGLIDEN
                     AHRLAEYLAVDPVGRKLPGYLRKLGQALADEKRAILGNIDQLAEHLEHMKKIIAAQQS
                     YAKVNGVTEVCTIEEIAETALAISEGALRNSNIEVVRRYEKLPPVLVDRHQIMQILVN
                     LIGNAKHALEDVVRPDRQLCVSISKVEGGVRIEVRDNGAGITAENLSKIFSHGFTTKK
                     TGHGFGLHNCANVAQQMDGSLTAYSDGPGKGASFVLRMAVE"
     gene            1823..>2046
                     /locus_tag="VM692_09130"
     CDS             1823..>2046
                     /locus_tag="VM692_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52372.1"
                     /translation="MSVILGAQPRPKAHVGANRVERRVKFRTSLGRGIGVQRRHRKRE
                     KHRMLDARREPSAKRLQAGRNLRELVRAGEV"
BASE COUNT          317 a          694 c          660 g          375 t
ORIGIN      
        1 actcgacggc catgcgcagc acgaagctcg cgcccttacc tggcccgtcg ctatacgcgg
       61 tcaggcttcc gtccatctgt tgcgccacat tggcgcagtt gtgcaggccg aagccgtggc
      121 ctgtcttttt cgtcgtgaaa ccgtgactga agatcttcga cagattctcg gccgtgattc
      181 ctgcaccgtt gtcgcggact tcgatgcgca cgccgccctc gaccttgctg atcgagacgc
      241 aaagctgccg atcgggccgg acgacgtcct cgagcgcgtg cttcgcgttg ccgatcaggt
      301 tcacgaggat ctgcatgatc tggtgacggt cgacgagcac cggcggcagc ttctcgtagc
      361 gccggacgac ctcgatgttg ctgttgcgca gcgcgccttc gctaatcgcg agcgcggtct
      421 cggcgatctc ctcgatcgtg catacctccg tgacgccgtt gaccttggcg tacgattgct
      481 gcgccgcgat gatcttcttc atgtgctcga gatgctcggc gagctggtcg atgttgccga
      541 gaatcgcgcg cttctcgtcc gccaacgcct ggccaagctt gcgcaggtaa ccgggcagct
      601 tgcgaccgac gggatccacg gccaaatact cggcgagccg gtgcgcgttc tcgtcgatga
      661 ggccgactgc ccgcgagacg ccctcgacgg gcagcgcagt gacggcctcg cgcgcgacgc
      721 tggcgccgac gttgacgctg ttcatgacgt tgccgacgtt gtgcagcacg cccgacgcga
      781 cctcggccat gccagcgcgc cgcgacaaat cgacgagccg cccttgcgcg ttctcgagct
      841 cgcgcgtgcg ttccttgacg aggtcctcca ggtgatcttt gtagcggctg agctcgccct
      901 ccgcgcggtt gcgttcggcg atctccttcg tcaaggtcac gttcgccgcc tcgatcgtgc
      961 ggctctgttg ttcttgtcgg ctatagagag tcgcgttgtc gatcgacacc gcgacctggg
     1021 acatcaagat gttcagcgcc tcgagcctgt ccggcgtgaa tgcaccggct acctggttgt
     1081 tctcgagata aacggcaccg atgagcttgc ccttgtgcgc gaccggcgcg cagagcaccg
     1141 acttcgggcg gcggttgacg acgtagacgt cgccgcgaaa cttgccttgc tgcgccgcgt
     1201 cgtcgagcac gacgagctcc gacgtgcgca acacgtaatt gacgatgccc gtggcgcacg
     1261 cggccgtgag ccgcagcgcc tcgccgccga gcacgcgcgc ctgcgcggcc gcggtcttga
     1321 cgccttgcac gaggaattcg ccgttcgttt cgagcacgag cacgacggac tccgccccgg
     1381 cgttctcgcg gatgatgtcc atgagcttcg tcagcaggcg ctccagcacg atctcgccgg
     1441 caatggcctg gctggctttc agcagggtcg cgaggtcgag ggcgtcggag cgctccgcgg
     1501 ccggaccggg cgtcgtcacg ctcgaggtaa cggacaccgt cgagccgctc gcggcaccca
     1561 agccgtgcct cgcgacgagg tcggccactt tgcccgtcgc gccccaggcc tcgtagccct
     1621 gcagcgcctt gtcgaggtag atgcgcgcga actcgggctt gccgtcgcgc agccaaaagc
     1681 gggccgcaag ctcggcagcc aaggcctcga cgttgatgta gccttgagca cgcgcggccg
     1741 cgatcgcgcg gtcgtagaga tcggcggcct cgatacgcgc gcccttgatg cgtgcgcact
     1801 cggcctcgac gagcagctgc atgtgagcgt gattctcggc gcacagcccc gcccaaaggc
     1861 gcatgtcggc gcgaatcgcg tcgagcgacg cgtcaaattc cgtacgtcgc tcgggcgtgg
     1921 catcggcgta caacgccgtc atcgcaagcg cgaaaaacat cgcatgctcg acgcgcgtcg
     1981 cgaacccagc gcaaaacggc tgcaggccgg ccgaaatctg cgcgaactcg tacgcgccgg
     2041 cgaagt
//
LOCUS       DATLEE010000426         4138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_483731, whole genome shotgun sequence.
ACCESSION   DATLEE010000426 DATLEE010000000
VERSION     DATLEE010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483731"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VM692_09135"
     CDS             <1..861
                     /locus_tag="VM692_09135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUQ52373.1"
                     /translation="LELGAGSGALAATLLDTFAQLGRNVRYSILEPSADLKQRQQRAL
                     ARFADRVRWLDRLPEIPFSGTVVANEVLDALPVARFVIEEDGPSALGVTAHDSGLAWQ
                     RARALPELVTAVRAIERALGRPLAVGYRSEACLVLPAWLRALGSSLERGSLLFVDYGL
                     VRSDYYHEQRADGTLICHYRQRAHADPFVYPGLQDITAWVDFSACAEAATAAEFAVAG
                     FTTQGQYLLSVLAALPPALAVDLASPQEQSALKTLILPGEMGERFKVMLLRKNVDGPA
                     LPGKDFRYRL"
     gene            944..1825
                     /locus_tag="VM692_09140"
     CDS             944..1825
                     /locus_tag="VM692_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52374.1"
                     /translation="MRRHDSIAWSLATAAALLAASAAAQSPSPDDARGAWMLGLGAEV
                     DEDESESLLATLYVGVGSSTWLTVVAGQSLSPADRADIEADTLAIGVDHRFDKVGFTL
                     EAERWGDSGALETQDFSSSVYFDRQRWRIGFGYETRDIEMPVTFTGPLGNTFRRTIDV
                     SADRLSFDARVAVGDSWQLYMGFAEHDYERKLNVLPRIATLNLLSASTLTLANSFLDH
                     ERYLAFERELGRVTLNVRAATDRSAIDDSKFDTLEAAVLFPLGSRVDLEVNIGNGRSD
                     FFDAGAYGGLLFLVYGR"
     gene            complement(1799..3046)
                     /locus_tag="VM692_09145"
     CDS             complement(1799..3046)
                     /locus_tag="VM692_09145"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HUQ52375.1"
                     /translation="MKVYEVGGAVRDALLGLPVEERDWVVVGASAAELTAQGFRRVGK
                     DFPVFLHPRTGEEYALARTERKTAPGYTGFEFDAAPTVTLEQDLQRRDLTINAIARAE
                     SGELIDPWSGRADLEARLLRHVSPAFSEDPLRVLRVARFAARFAPLGFTVADATMQLM
                     KEIVAAGEMEALRPERVWQETVKALATERPDVYFETLRAANALQRIFPEVDALFGVPQ
                     PAQWHPEIDTGVHTLMALRVAAQLSRSETVRFAVLTHDLGKATTPQDLLPRHHGHEQR
                     SEELLEKLCERFPVPNKFRELALLVARHHGNVHKAAELKPQTVLRMIMAADGLRQPER
                     FEEFLLACEADARGRKGLEDRAYSQADRLRSALRAARGVEAAKVRAERNVDGAALGEA
                     LHDARLAAIKADFRASAAVNQEQ"
     gene            3090..>4138
                     /locus_tag="VM692_09150"
     CDS             3090..>4138
                     /locus_tag="VM692_09150"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HUQ52376.1"
                     /translation="MNSIGRAFALCAVAVLTAGCAAPYYWQAIGGQLELLRKREPIEE
                     VIADPQADPKLKTTLTRITEIRRFAVDELGLPSNDSYTSYVALDRPYVVWNVVATEEF
                     SVDPRRWCFPFAGCVAYRGYFDKAAADRFAANLANDGLDVHSGGSTAYSTLGYFADPV
                     LSTMVNGGDQYVASLLFHELAHQKLYIKDDSEFSESFAMVVEELGTERWLTQHGTPAD
                     LDLYRVRRERRAQFGDLVAAQQRRLRAVYGSGAEPAELRAHKERAFATLQTEYVAMKA
                     GWGGNTDYDAWFAQPLNNATLASVATYTRWLPALRWRVQEVGLTAFYVDAAALEKLDA
                     EQREKELLAWDARART"
BASE COUNT          660 a         1380 c         1381 g          717 t
ORIGIN      
        1 ctggagctcg gtgcgggcag cggcgcgctg gccgcgaccc tgctcgacac gttcgcgcag
       61 ctcggtcgca acgtgcgcta ctcgatcctc gagccgagcg cggacttgaa gcaacgacaa
      121 caacgcgcgc tcgcgcggtt cgccgatcga gtgcggtggc tcgatcgcct cccggagatt
      181 ccgttcagcg gcaccgtcgt cgcgaacgaa gtcctggacg cgctgccggt agcgcggttc
      241 gtcatcgagg aagacggacc gagcgcgctc ggcgtcacgg cgcacgactc aggtctcgca
      301 tggcaacgcg ctcgcgcgct gcccgagctc gtcacagccg tgcgcgcgat cgagcgcgcg
      361 ctcggccggc ctttggcagt gggctaccgc tcggaggcat gcctagtttt gccggcctgg
      421 ttgcgcgccc tcggctcgag cctcgagcgc ggctcgctgt tgttcgtcga ctatgggctc
      481 gtgcgcagcg actactatca cgagcagcgc gcggacggga cgctgatctg ccactaccgc
      541 cagcgcgcgc acgccgaccc gttcgtctat ccggggctgc aagacatcac ggcatgggtg
      601 gacttcagcg catgcgccga ggcggcgacg gctgcggaat tcgccgtcgc cgggttcacg
      661 actcaaggtc agtacttgct cagcgttctc gcggcgctcc cgccggcgct ggcggtcgac
      721 ttggcatcgc cgcaggagca aagcgcgctg aagacgctga tcttgcccgg cgagatgggc
      781 gagcggttca aggtcatgct gctgcgcaag aacgtcgacg ggcccgcgct accgggcaag
      841 gattttcgct atcggctctg agccgctgag gtcgcgccgt ttcacagaac gacgaagcac
      901 gacttgttaa gatcgccgcg tcactaacgc cggagtgccc tccatgcggc gtcacgactc
      961 gatcgcttgg tcgcttgcta ctgcggcagc gctgctagcg gcatcagccg ccgcgcaatc
     1021 tccgtcgccc gacgatgcgc gcggcgcgtg gatgctcggg ctcggtgccg aggtcgacga
     1081 ggacgaaagc gagagcctgc tcgcgacgct gtatgtcggc gtcggctcga gcacgtggtt
     1141 gacggtcgtc gccggccaga gcttgtcgcc cgcggaccgc gccgacatcg aagcggacac
     1201 gctcgcgatc ggcgtcgatc atcgcttcga caaagtcggg ttcacgctcg aagcggagcg
     1261 ctggggcgac tccggggcgc tcgaaacgca ggacttttcg agctccgtct atttcgatcg
     1321 gcaacgctgg cgcatcggct tcggctacga gacgcgcgac atcgagatgc cggtcacgtt
     1381 cacggggccg ctgggcaaca ccttccgccg cacgatcgac gtctcggccg atcgcttgtc
     1441 gttcgacgcg cgcgtcgcag tcggcgatag ctggcagctc tacatggggt tcgcggagca
     1501 cgactacgag cgcaaactca acgtgctacc gcggatcgcg actttgaatc tgctgagtgc
     1561 ctcgacgttg acgcttgcca atagctttct cgaccacgag cgctacctcg cgttcgaacg
     1621 cgagcttggc cgtgtcacat tgaacgtgcg cgccgcgacc gaccgctccg ccatcgacga
     1681 ctcgaagttc gacacgctcg aggcggccgt gctgtttccg ctcggctcgc gcgtcgatct
     1741 cgaggtcaac atcggcaacg gtcggtccga cttcttcgac gccggagcgt atggcggcct
     1801 actgttcttg gtttacggcc gctgacgccc tgaagtcggc tttgatggcg gcgagccgcg
     1861 cgtcgtgtaa agcttcgcca agcgccgcgc cgtcgacgtt gcgctccgct ctgaccttcg
     1921 ctgcctcgac tccgcgggcg gcgcgcaacg ctgaacgcag ccgatcggct tggctgtagg
     1981 ctcggtcctc gaggcctttg cgcccccgag catccgcctc gcaggccagc aggaactcct
     2041 cgaatcgctc cggctgccgc aagccgtcgg cggccatgat catgcgcagc accgtctgtg
     2101 gcttgagctc ggccgccttg tgcacgttgc cgtgatgtcg tgccacgagc agcgcaagct
     2161 cgcgaaactt gttcggcacc gggaagcgct cgcatagctt ctcgagcagc tcttcggagc
     2221 gctgctcgtg gccatggtgt cgcggcaata ggtcttgcgg cgtcgtcgcc ttgccgaggt
     2281 cgtgcgtcaa caccgcgaag cgcacggtct cgctgcgcga caattgcgcc gccacgcgca
     2341 gcgccatcag cgtgtgcacg ccggtgtcga tctcggggtg ccactgcgcg ggctgcggaa
     2401 cgccgaacag cgcatcgact tcggggaaga tgcgctgtag cgcgttggcc gcgcgcagcg
     2461 tctcgaagta aacgtcgggg cgctccgtcg ccagcgcctt gacggtctct tgccagacgc
     2521 gctcgggacg caacgcctcc atctcgcccg ccgctacgat ctccttcatc aactgcatcg
     2581 tcgcgtcggc gaccgtgaag ccgagcgggg cgaagcgcgc cgcgaaacgt gcgacgcgca
     2641 aaactcgcag cggatcctcg ctgaacgccg gcgagacgtg ccgcagcagt cgcgcctcga
     2701 ggtcggcacg gcccgaccac ggatcgatga gctcgccgct ctcggcgcgc gcgatggcgt
     2761 tgatcgtcaa gtcgcgacgc tgcaggtctt gctcgagcgt tacagttggc gccgcgtcga
     2821 actcgaagcc cgtgtagccg ggcgcggttt tgcgctcggt gcgcgccagc gcgtactctt
     2881 cgcccgtgcg cgggtgcagg aagaccggaa agtccttgcc gacgcgccga aagccttggg
     2941 cggtgagctc ggcggccgac gcgccgacga cgacccagtc gcgctcctcg accggcaggc
     3001 cgagcagtgc atcacggact gcgccgccca cctcgtagac tttcatgccc gcatcatacc
     3061 gtagggctgc acatgctaag gtcgccgcca tgaattcgat cggcagagca tttgcgttgt
     3121 gtgcagtagc agtgctgact gcgggatgcg ccgcgccgta ttactggcaa gccatcggcg
     3181 ggcagctcga gctgttgcgc aagcgcgagc cgatcgagga agtcatcgcc gacccgcaag
     3241 ccgatcccaa gctgaaaacc acgttgacgc gcatcacgga gatacgccgg ttcgctgtcg
     3301 acgagctcgg cttgccgagc aacgacagct acacgagcta cgtcgcactc gatcggccgt
     3361 acgtcgtctg gaacgtcgtc gcgacggaag agttctccgt cgatccgcgg cgctggtgtt
     3421 ttccgttcgc gggttgcgtg gcgtatcgcg gctacttcga caaggccgcc gcggatcgct
     3481 tcgctgccaa cctcgccaac gatggcttgg acgtgcacag cggcggctcg acggcgtact
     3541 cgacgctcgg ctacttcgcg gatccagtgt tgagcacgat ggtcaacggc ggcgatcaat
     3601 acgtggcgag cttgctgttt cacgagctcg cgcatcaaaa gctctacatc aaggacgaca
     3661 gcgagttcag cgaatcattt gcgatggtcg tggaggagct cggcacggag cgctggctga
     3721 cgcagcacgg gaccccggcg gatctcgacc tttaccgagt tcggcgcgag cgccgcgcgc
     3781 agttcggcga cctcgttgcc gcgcagcagc gacgcttacg cgcggtctac ggaagcggcg
     3841 ccgagcccgc cgagctacgc gcgcacaagg aacgagcgtt tgcgacgctg cagaccgagt
     3901 acgtcgcgat gaaggcgggt tggggcggca acaccgacta tgacgcgtgg ttcgcccagc
     3961 cgctcaacaa cgccacgctg gcatccgtcg cgacgtacac gcgttggctg ccggcgctgc
     4021 gctggcgtgt tcaagaggtc ggccttacgg cgttctatgt cgacgcggcg gcgctcgaga
     4081 agctcgatgc cgagcagcgc gagaaagagt tgctcgcctg ggatgcgcgt gcgcgcac
//
LOCUS       DATLEE010000427         3035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_485513, whole genome shotgun sequence.
ACCESSION   DATLEE010000427 DATLEE010000000
VERSION     DATLEE010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485513"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VM692_09155"
     CDS             complement(<1..685)
                     /locus_tag="VM692_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HUQ52377.1"
                     /translation="MIIRTRVFAPVLALLALSIAGPVWSQSSLNMPVGVTSTSRDVYD
                     LHMLILWVCVIIGAGVYGAMIAAIVKFRKSKGAVAATWTHNGTVEVIWTVVPAVILVA
                     MAVPAAETLIRMEDTRDSELTIKVTGFQWRWQYDYIDQGVRFFSTLDRESNAARRLDS
                     GIDPDSVDNYLLKVDNELVVPVDTKVRLLITGADVLHAWWVPALAVKKDAVPGFINES
                     WFTADTEGTY"
     gene            complement(788..1678)
                     /locus_tag="VM692_09160"
     CDS             complement(788..1678)
                     /locus_tag="VM692_09160"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUQ52378.1"
                     /translation="MSISLDAALPEKRAARRAFDRARAFDDACFLHDEARSRLLERLE
                     LVRLTPQVAIDLGCATARGANALAVRYPAARVLAVDSSGGMLRSAAGHARSGVSVLGG
                     DAELLPLQSHCADLVLANLVLPWCRPDRLFAESARVLAEGGALLFATLGPDSLQEVRA
                     AFGGVDDHIHVHAAFDMHDLGDLALAAGLAEPVLDVDRLEVTYADVAGLVRDLRGAGA
                     VNVAGGRRRSLTGRERWRRFEARLVPPEGRLAVTVELILGQAWGRGRVDREGGHGPRG
                     GGEVLVPLDRIGRRFPSGNT"
     gene            complement(1703..2752)
                     /gene="bioB"
                     /locus_tag="VM692_09165"
     CDS             complement(1703..2752)
                     /gene="bioB"
                     /locus_tag="VM692_09165"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HUQ52379.1"
                     /translation="MTPEPRSDWTRAEVESLFELPFNDLLYAAQSVHRQNFDPNAVQV
                     STLLSIKTGACPEDCAYCPQSIRFETGVTNHDLMPLDEVRTAARRAKDAGATRFCMGA
                     SYRGPKDSQLEPILAMIEEVKAMGLETCATLGLLRPGQAERLAAAGLDYYNHNLDSSA
                     EFYEKIISTRTYEDRLETLERVRAAGVKVCCGGIVGMGETRADRVGLLHTLATLKPQP
                     ESVPINELVPILGTPLAAAGPLDPFEFVRCIAVARILMPRSHVRLSAGRSDFSDELQA
                     LCFFAGANSIFYGEKLLTTGNPDTAADRRLFERLGLAPEDAGMDARGRATQGAVADAT
                     ADARGEAVAEGPAAH"
BASE COUNT          513 a         1040 c         1006 g          476 t
ORIGIN      
        1 gataagtccc ctccgtatcc gcagtgaacc aggactcgtt gatgaagccg ggcacggcgt
       61 ctttcttgac ggccaacgcc ggcacccacc atgcgtgcag cacgtcggcg cccgtgatca
      121 gcagccggac tttcgtgtcg acgggtacca cgagctcgtt gtcgaccttc agcaaatagt
      181 tatcgacgct gtccgggtcg atacccgagt cgagcctgcg agccgcattg ctctcgcggt
      241 cgagcgtgct gaagaaccgc acgccttggt cgatgtagtc gtattgccag cgccattgga
      301 aacccgtgac cttgatcgtg agctcggagt cgcgcgtgtc ttccatgcga atcagcgtct
      361 ccgccgccgg cactgccatg gcgacgagga tgacggccgg cacgacagtc caaatgacct
      421 cgacggtgcc gttgtgcgtc cacgtggccg cgacggcgcc cttcgactta cggaatttga
      481 cgatggccgc gatcatggcc ccatagacgc cggcgccgat gatcacgcat acccacagga
      541 tcagcatgtg gaggtcgtag acgtcgcggc tcgtcgacgt cacgccgacg ggcatgttga
      601 gcgaggattg agaccagacg ggtccggcga tgctcaaagc gagcagagcc aggacggggg
      661 caaacactcg agtcctgata atcatgcgtc ctgcaattat tgatgaggcg gccgcctgcc
      721 ggcgccgcgc ggcgcgaatt ctagcagaac aaaggcggga gccaggaacg gcgcggcaaa
      781 taggcgccta ggtatttccc gaagggaacc gccgcccgat ccggtcgagc ggcacgagga
      841 cctcgccgcc gccgcgcggg ccgtgtcccc cttcacggtc tacacggccg cgcccccagg
      901 cttggcccaa gatcagctcg acggtgaccg cgagtcggcc ttccggcggc acgaggcgcg
      961 cttcgaatcg tcgccagcgc tcgcggcccg tcagactgcg gcggcggccg ccggccacgt
     1021 tgacggctcc cgcgccccgc aggtcgcgga cgagccccgc gacgtccgcg taagtcacct
     1081 cgagccgatc gacgtcgagg accggctccg cgaggccggc ggccagcgcg aggtcgccca
     1141 aatcgtgcat gtcgaaggct gcgtggacgt ggatatggtc gtcgacaccg ccgaatgccg
     1201 cgcgcacctc ttgcagcgag tccgggccca acgtcgcgaa cagcagggcc ccgccctcgg
     1261 cgagcacccg cgcgctctcg gcgaacaggc gatccggcct acaccacgga aggacgaggt
     1321 tggccaacac gaggtctgcg cagtgcgact ggagcggcaa cagctcggcg tccccgccga
     1381 gcacgctgac gccgctccgc gcgtgcccgg cggccgagcg caacatgccg ccgctcgagt
     1441 cgacggcgag cacacgcgcg gccggatagc ggacggcgag tgcgttggcg ccgcgcgccg
     1501 tggcgcaacc gagatcgatg gcgacttgcg gcgtaaggcg cacgagctcg agccgctcga
     1561 gcaatcggct gcgcgcctcg tcgtgcagga agcaggcgtc gtcgaaggcg cgcgcgcggt
     1621 cgaacgcgcg tcgcgcggcc cgcttctcgg gcaacgcggc atcgaggctg atcgacacga
     1681 gaccttgagc gcgacaccgc gactaatgcg cggcggggcc ctcggcaacc gcttcaccgc
     1741 gggcatcggc ggtcgcgtcg gcaaccgctc cctgcgttgc tctaccgcgg gcatccatgc
     1801 ccgcgtcctc gggagccaag cccaggcgct cgaacaggcg ccggtccgcg gccgtgtcgg
     1861 ggttgcccgt agtcagcagc ttctcgccat agaaaatcga attcgcgccg gcgaagaagc
     1921 acagcgcctg cagctcgtcg ctgaagtcgc tgcggcctgc cgacagccgc acgtgcgagc
     1981 gcggcatcag aatgcgcgcg acggcgatac agcgcacgaa ctcgaacgga tcgagagggc
     2041 ccgccgccgc caacggcgtt cctaaaatcg gtacgagctc gttgatcggc acgctctcgg
     2101 gctgcggctt cagcgtcgcg agcgtgtgca gcaaaccgac gcgatcggcg cgcgtctcgc
     2161 ccatgccgac gatgccgcca caacagactt tcacgcccgc cgcgcgaacg cgttcgagcg
     2221 tctcgagccg gtcttcatac gtgcgagtgc tgatgatctt ctcgtagaac tccgccgacg
     2281 agtcgaggtt gtggttgtag tagtcgaggc cggcagccgc gagacgctcg gcctgacccg
     2341 gcctcagcaa tcccaacgtg gcgcaggtct cgaggcccat ggccttgacc tcttcgatca
     2401 tggcgaggat cggctcgagc tgcgaatcct tgggcccgcg atacgacgcg cccatgcaga
     2461 agcgcgtcgc acccgcgtcc ttcgcgcggc gcgcggccgt gcgaacttcg tcgagcggca
     2521 tcaggtcgtg attcgtcacg ccggtctcga agcggatgct ctgcgggcag tacgcgcagt
     2581 cttcggggca cgcacccgtc ttgatcgaca acagcgtcga gacctgcacg gcatttggat
     2641 cgaagttctg ccgatgcacc gactgcgccg cgtacaacag atcgttgaac ggcagctcga
     2701 acagcgactc tacttctgct cgtgtccagt cgctgcgcgg ctccggcgtc acgcggctcg
     2761 atagtcccgg aacgggcacg ccgcgagccg tccccctttg gggactaccc aggggcattt
     2821 ccttctcgac tgccgacatt cgttgctcgc tctaacggcg ccgtcgcgcg acgctggagc
     2881 atcacaaacg acggcgagcg ctgtcaacca aagggagcgg cattggttta cggttatcta
     2941 cgccggctct tcgtcgagag ctgccctggc tgcattgggc cgtcggaggg cggcttctgc
     3001 gccgtctgcg cggccgcgtt tccgctcgtg cgtga
//
LOCUS       DATLEE010000428         2704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1229188, whole genome shotgun sequence.
ACCESSION   DATLEE010000428 DATLEE010000000
VERSION     DATLEE010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229188"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VM692_09170"
     CDS             <1..667
                     /locus_tag="VM692_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52380.1"
                     /translation="MVIGVLGGAAFVAVYNYMIQYREYAGPLSEYFTGDTLRYYLYTG
                     AANTDQPYIGRFDSIEIALDHISQNPLKLAFGYGAGNVSESFLPAFDGQYAEYYVRFG
                     VGQTQVTQFLWEVGLVGLLAYLFLFYLVARDALALARSSDSGAPLGQLWTVAMIVMTF
                     GLIYKSLLSMNDFGYLFWYFSGVVASRAVAVRQGVRKRAPQLPPSWRLAMDGPSAAMK
                     ARP"
     gene            798..1604
                     /locus_tag="VM692_09175"
     CDS             798..1604
                     /locus_tag="VM692_09175"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUQ52381.1"
                     /translation="MRHNRVQVEKQSVPAGARLQAAEPSAVAAPAAPAAPQRSSIRHA
                     IREVAFALSRRFRARRAKRFRSVFELTEHTRILDLGGWDGEHIRMVLEGSPVRPENVY
                     VADVNEKALERARAHGHRTALLPQDGTVPFPDGFFDVVFCSSVIEHVTVPKSEVWQHR
                     GELFGRKAIAHQRAFADEILRISKSFWVQTPARSFFLETHAWLPFLGWLPRSAQVAIL
                     SCTRHFWLSRHPPDYYLLTRGHMEALFPGARILSERWLGMTKSWIAVRQV"
     gene            1636..>2704
                     /locus_tag="VM692_09180"
     CDS             1636..>2704
                     /locus_tag="VM692_09180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ52382.1"
                     /translation="MTATLLSVNNYYYPRGGAEVVFFRHNGMLQDAGWSVVPFAMNHR
                     MNVGGAERPEFVAELEYGRESDTLATRLRKGLKSVYSFEARGKLGRLIDRSSPDLCHA
                     HNVYHHLSPSILGLVRARGIPLVMTLHDLKLACPAYSMLSHDGVCERCRDGRLYQVVT
                     HRCMKGSFALSALVMLESYVHRLLGSYLRNVDRFLVPSRFYLDKLVDWGFPAERFEYV
                     PNFVAAESYEACYTPGQRFVYFGRLSREKGIATLIRAAAAAGVGLDIIGTGPQEAELR
                     ALAGDHDVRFLGFLTGAKLNAAVGAARAVVVPSEWYENAPLSVLEGAALGKPLIVARI
                     GGLPELVVENESGWSFESRSVE"
BASE COUNT          417 a          897 c          895 g          495 t
ORIGIN      
        1 catggtcatc ggggtcctcg gcggggctgc gttcgtcgcg gtctacaact acatgatcca
       61 gtatcgcgag tatgccgggc cgttgagcga gtacttcacg ggcgacacgc tgcgctacta
      121 cctctacacg ggcgccgcga acaccgacca gccgtacatc ggccgcttcg acagcatcga
      181 gatcgcgctc gaccacatca gccaaaaccc gctgaagctc gcgttcggct acggcgcagg
      241 caacgtgtcg gagtcgttct tgcctgcgtt cgacgggcaa tacgccgagt actacgtacg
      301 gttcggcgtc gggcagacgc aagtcacgca gttcctctgg gaagtcggtt tggtcggcct
      361 gctcgcgtac ctgttcctgt tctacctcgt ggcccgcgac gcactagcgc tggcgcgctc
      421 gagcgactcc ggcgcgccgc tcggccagct ctggaccgtc gcgatgatcg tcatgacgtt
      481 cggcctcatc tacaagagcc tgttgtcgat gaacgacttc ggttacctgt tttggtattt
      541 ctcgggagtc gtggcgagcc gcgcagtcgc cgtccgccag ggcgtgcgca agcgcgctcc
      601 gcaattgccg ccgagctggc ggctcgcgat ggacggccca tctgccgcca tgaaggcgcg
      661 gccgtgacgc gcgccgcacg ccgccattgg cacgattcgt gctcttttct ccgcggcagg
      721 ggttcgttgc agggagcaac agtggccaac aaacaacgcg agcatagcga gcgccaccgc
      781 gggcgcgcgg cttagccatg cgccacaatc gagtgcaggt cgagaagcag agcgtgcctg
      841 cgggcgcacg gctgcaagcc gcggagccga gcgcggtggc cgcgccggcc gcgccggccg
      901 caccgcagcg ttcctccatc aggcatgcga ttcgcgaggt ggcgttcgcg ctgtcgcggc
      961 ggtttcgcgc gcggcgggcc aagcggttcc gttcggtctt cgagctaacc gagcacacgc
     1021 gaatactcga tctcggcggt tgggacggcg agcacattcg catggtgctc gagggctcgc
     1081 ccgtgcgccc cgaaaacgtc tatgtcgcag acgtcaacga gaaagcgttg gaacgtgcca
     1141 gggcccacgg ccatcgcacg gcgctgctgc cgcaggacgg cacggtgccc tttcccgacg
     1201 gtttcttcga cgtcgtgttc tgctcgtccg tgatcgagca cgtcacggtg ccgaagagcg
     1261 aggtctggca gcaccgcggc gagctgttcg gtcgcaaagc gatcgcgcat cagcgcgcgt
     1321 tcgcggatga aattttgcga atctcgaaat cgttctgggt acagacgccg gcgcgatcgt
     1381 tctttctcga aacgcacgcc tggttgccgt tcctgggctg gctgccgaga tccgcgcagg
     1441 tcgcgatctt gagctgcacg cggcatttct ggctgtcacg gcacccgccc gattactacc
     1501 tactgactcg cggtcacatg gaggcgctgt ttccgggcgc tcgcatcctc agcgaacgct
     1561 ggctgggcat gacgaagtcc tggattgccg tgcgccaagt ctgacgcgag gcacggccgc
     1621 ccagagtcag caccgatgac cgcgacgtta ctcagcgtca acaattacta ctacccgcgc
     1681 ggcggagccg aggtcgtctt cttccgccac aacggcatgc tgcaggacgc cggctggtcc
     1741 gtcgtgccgt ttgcgatgaa ccatcgcatg aacgtcggcg gcgccgagcg ccccgagttc
     1801 gtcgccgagc tcgagtatgg tcgcgagtcg gacacgctcg cgacgcgcct tcgcaagggt
     1861 ctgaagtccg tctattcgtt cgaggcgcgc ggcaagctcg ggcggctcat cgatcgctcg
     1921 tcgcccgacc tgtgccacgc gcacaacgtc tatcaccact tgtcgccgtc gatcctcggg
     1981 ctcgtgcgcg cgcgcggcat accgctcgtc atgacgctgc acgacctgaa gctcgcctgc
     2041 cccgcctact cgatgctgtc gcacgacggc gtgtgcgagc gctgccgcga cggccggctc
     2101 tatcaagtcg ttacgcatcg ctgcatgaag ggcagtttcg cgctgtcggc gctcgtgatg
     2161 ctcgagagct acgtgcatcg cctgctcggc agctatttgc gcaacgtcga tcggttcctc
     2221 gtgccgagcc gcttctacct cgacaagctc gtcgactggg gcttcccggc cgaacggttc
     2281 gagtacgtgc cgaactttgt ggcggccgag tcgtatgagg cctgctacac gcccggccag
     2341 cgcttcgtct atttcgggcg cctgtcgcgc gaaaagggaa tcgcgacgct gattcgcgcg
     2401 gcggccgcgg ccggagtcgg gctcgacatc atcggcacgg ggccgcaaga agccgagctc
     2461 cgagcgctgg caggcgacca cgacgtgcgg ttcctcgggt tcttgacggg cgcgaagttg
     2521 aacgcggccg tcggcgcggc ccgcgccgtc gtcgtgccgt cggagtggta cgagaacgcg
     2581 ccgctgtccg tgctcgaagg ggccgcgcta ggcaagccgc tgatcgtcgc gcgcatcggc
     2641 ggtctgccgg agctcgtcgt cgagaacgag tcgggctgga gcttcgagtc gcgctccgtc
     2701 gagg
//
LOCUS       DATLEE010000429         4095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_486529, whole genome shotgun sequence.
ACCESSION   DATLEE010000429 DATLEE010000000
VERSION     DATLEE010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486529"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..2298
                     /locus_tag="VM692_09185"
     CDS             115..2298
                     /locus_tag="VM692_09185"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HUQ52383.1"
                     /translation="MAEYVTHGGIKIAKSIHDLVRDEIAPGTGIAPDTVWTLLDSIVR
                     TLGPRNRALLDKRDALQAKIDAWLAERQGAALEPKATAAFLSEIGYLVPVGPAFEVTT
                     ANVDPEIASLAGPQLVVPVDNPRYALNAANARWGSLYDALYGTNVIAEDGGAEKGSGY
                     NPVRGERVIAKANEFLDQAVPLSSGKWADVTALDSSAGQLACGLRDGRSVGLAEAAQF
                     VGFRGTGKHLTNVLLRHHGLHIDIVIDASHPIGKQHPAGIKDVVLESAITTIMDCEDS
                     VAAVDAADKTVVYRNWSGIMRGTLTASVAKGNGTIERRLNPDREYTAPDGTAFKLPGR
                     SLLLVRHIGAHAVTDAVTDVAGKPIAETFLDCVLTTLAALHDLKGSGALHNSRTGSLY
                     IVKPKQHGPEEVALSVELFATVERAFGLPPNTLKIGIMDEERRTTVNLKECIRVSRDR
                     IIFINTGFLDRTGDELHTHMEAGPMIPKPEIKGARWIAAYEDWNVRVGLETGLPGHGQ
                     IGKGMWAMPDEMRAMIDTKQGHPKAGATTAWVPSPTAATLHAIHYHQIDVAARQREIA
                     QQGGATPELLDGILTPPLLTRQLTSAEVARELDNNAQGILGYVVRWVEQGVGCSKVPD
                     INDVGLMEDRATLRISSQHIANWLRHGIVDRTQVRATFERMAEVVDRQNAGDRNYRNM
                     APDFASSVAFQAALDLVFNGLKEPNGYTEHTLARRRREFKNKNGR"
     gene            complement(2295..3170)
                     /locus_tag="VM692_09190"
     CDS             complement(2295..3170)
                     /locus_tag="VM692_09190"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUQ52384.1"
                     /translation="MNRITAPTTQRRQLIDFLKGCRARLTPTQVGLPDTNRRRTPGLR
                     REDVAALAGVSVTWYTWLEQGRDIQVSADVLERISTTLRMSADEREYLFALVQHRPAP
                     PIATRAEAVSPAMKRMLDALAVPAIVLTSRWDVIAWNPLIKVIRDYGALPLERRNLLR
                     ILLVEDEIYRQNPELYEATARRILAKFRVDYSQAPGDPAFEELIADLSASCPIFDRLW
                     ALAEVVGTHEAVVTHPRAGLTFEHSSYVPEGYPKQRLIIFTPCNEASVAKVREAVAQT
                     PMPTGGARRSSAHVN"
     gene            3396..3782
                     /locus_tag="VM692_09195"
     CDS             3396..3782
                     /locus_tag="VM692_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ52385.1"
                     /translation="MSAQQPLTTASLAAWLGRYEQAWEQRDPAQAAALFTDNAPYHEM
                     PFDAPKAGRAGIRDYWATVTADQRNIDFKSEVVAVNGQTGVARWSASLTSASSGARVE
                     LDGVFILQFDRNGLCSELREWWHVRM"
     gene            3981..>4095
                     /locus_tag="VM692_09200"
     CDS             3981..>4095
                     /locus_tag="VM692_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004015171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HUQ52386.1"
                     /translation="MSFDSLGLAPALLRAVQEQGYTQPTPIQGRVIPVVLEG"
BASE COUNT          751 a         1358 c         1334 g          652 t
ORIGIN      
        1 gcgcggccag taattctcgc tcccaaaacg agattccagc cattatcctc atgggcactt
       61 aaacggcgcg ttacgacgcg accgcaccga aacggtttgt cgagcggagc atcaatggcc
      121 gagtacgtca cccacggcgg gatcaaaatc gccaaatcca tccacgacct cgtgcgtgac
      181 gagatcgcgc cgggcacggg tatcgcaccg gacaccgtgt ggacgctgct cgattcgatc
      241 gtccgcacgc tgggcccgcg caaccgcgcg ttgctcgaca agcgcgacgc gctgcaagcg
      301 aagatcgacg cctggctggc cgagcggcaa ggcgctgcgc tggagccgaa ggcgaccgcg
      361 gcgttcctga gcgagatcgg ctacctcgtg cccgtcggcc ccgcgttcga ggtcacgacg
      421 gcgaacgtcg atcccgagat cgcgagcttg gccggaccgc agctcgtggt gcccgtcgac
      481 aacccgcgct atgcgctgaa tgcggcgaac gcacgctggg gcagtcttta cgacgcgctc
      541 tacggcacca acgtcatcgc cgaggacggg ggcgccgaga aaggtagcgg ctacaacccc
      601 gtgcgcggcg agcgcgtcat cgccaaagcg aacgaatttt tggaccaagc cgtgccgctg
      661 agctcgggca aatgggccga cgtcacggca ctcgactcga gcgccgggca gctggcgtgt
      721 ggcctacgcg acggccgctc ggtcggcctc gccgaggcgg cgcagttcgt cggctttcgc
      781 ggcaccggca agcatctgac gaacgtcctg ctgcgccatc acgggttgca catcgacatc
      841 gtcatcgacg cgtcgcatcc gatcggcaag cagcacccgg ccggcatcaa ggacgtcgtg
      901 ttggaaagcg cgatcacgac gatcatggac tgcgaggatt ccgtcgcggc cgtcgatgcg
      961 gccgacaaga cggtcgtgta ccgcaactgg tccggcatca tgcgcggcac gctgacggct
     1021 tccgtcgcga agggcaacgg cacgatcgag cgtcggctca atcccgatcg cgaatacacc
     1081 gcccccgacg gcacggcgtt caagctaccg ggccgaagcc tgctgttggt gcggcacatc
     1141 ggcgcgcatg cagtgacgga tgccgtcacg gatgttgccg gcaagcccat cgccgaaacg
     1201 ttcctcgatt gcgtgctgac gacgttggcc gcgctgcacg acctcaaggg ctcgggagcg
     1261 ctacacaaca gccgcaccgg cagcctctat atcgtcaagc cgaagcagca cggccccgag
     1321 gaagtcgcgt tgtccgtcga gctgttcgcg accgtcgagc gagcgttcgg cttgccgccg
     1381 aacacgctga agatcggcat catggacgaa gagcgacgca cgacggtcaa cctcaaagag
     1441 tgcatccgcg tctcgcgcga ccgcatcatc ttcatcaaca caggtttcct cgatcgcacc
     1501 ggcgacgagc tgcacacgca catggaagcc gggccgatga tcccgaagcc cgagatcaag
     1561 ggcgcgcgct ggatcgcggc ctacgaggac tggaacgtgc gcgtcggtct cgagacgggc
     1621 ctgcccggcc acggtcagat cggcaaaggc atgtgggcca tgcccgacga gatgcgcgcg
     1681 atgatcgaca ccaagcaggg gcatccgaag gccggcgcga cgacggcgtg ggtgccctcg
     1741 ccgaccgccg cgacattgca cgcgattcac tatcatcaaa ttgacgtcgc ggcgcggcag
     1801 cgcgagatcg cgcagcaagg cggcgcgacg ccggagctgc tcgacggcat tctcacgccg
     1861 ccgctgctga ctcggcagct gacgtccgcc gaggtcgcgc gcgagctcga caacaacgcc
     1921 cagggaattc tcggttacgt cgtgcgctgg gtcgagcaag gcgtcggctg ctcgaaagtc
     1981 cccgacatca acgacgtcgg gctcatggaa gaccgcgcca cgttgcgaat ctcgtcgcaa
     2041 cacatcgcga actggctgcg tcacggcatc gtcgatcgca cgcaagtacg cgcgacgttc
     2101 gagcggatgg cggaagtcgt cgatcggcag aacgccggcg accgcaatta ccgcaacatg
     2161 gcgcccgact tcgcgagcag cgtcgcgttc caggccgcgc tcgacttggt gttcaacggg
     2221 ctcaaggagc cgaacggcta cacggagcac acgctcgcgc ggcgtcggcg cgagttcaag
     2281 aacaagaacg ggcgctagtt gacgtgggcc gatgagcggc gcgcgccgcc cgtgggcatt
     2341 ggcgtctgcg cgacggcttc gcgcaccttc gcgacgctcg cttcattgca cggcgtgaag
     2401 atgatcaggc gttgcttcgg atagccctcg ggcacatacg atgagtgctc gaatgtcagg
     2461 ccggctcgcg ggtgcgtcac gaccgcttcg tgcgtgccga caacctcggc gagggcccac
     2521 aagcggtcga agatcggaca cgacgcggac aagtccgcga tcagctcctc gaacgccgga
     2581 tcgccgggcg cttgactata gtcgacgcgg aacttcgcga ggatgcgccg cgccgtcgcc
     2641 tcgtagagtt ctggattctg ccggtaaatc tcgtcttcca cgagcagaat gcgcagcaag
     2701 ttgcgtcgct cgagcggcag cgcgccgtag tcgcggatga ccttgatcaa cgggttccac
     2761 gcgatgacgt cccagcgcga cgtcaggacg atggccggga cggcgagcgc atcgagcatg
     2821 cgtttcatcg ccgggctcac ggcctcggcg cgcgtcgcga tcggcggcgc gggccggtgc
     2881 tgcacgagcg cgaacaaata ctcgcgctcg tcggccgaca tgcgcagcgt cgtcgagatg
     2941 cgctcgagca cgtcggcgga cacctgaatg tctcgcccct gctcgagcca cgtgtaccac
     3001 gtgacgctga cgccggccaa cgcggccacg tcctcgcgcc gcaggcccgg cgtacggcgc
     3061 cgattcgtgt ccggcagccc cacctgcgtc ggcgtcagac gcgcacggca gcccttcaag
     3121 aaatcgatga gctggcgccg ttgagtcgtt ggcgcggtga tcctgttcaa agtcggttcc
     3181 ttgggcgggc gagctgcgcg gcggggcttt gcgtgatgtt atagcagcat tcgtaccaga
     3241 cccaagcagt tgagccggga aagcacggcg tgccaacctt atacgcaccg tcgccgcccg
     3301 agaggacact gatgcaaggc ccaaccgaaa caacggctaa catgcgcgtt atccgccgct
     3361 gtttgacgct cgcgctgctc tttgcaggag gttccgtgtc cgcacaacaa ccgcttacga
     3421 ccgcttcgct cgcggcttgg ctcggccgtt acgagcaagc ctgggagcag cgcgatcccg
     3481 cgcaagccgc agcgctgttc acggacaacg cgccgtatca cgagatgccg ttcgacgcgc
     3541 cgaaggccgg ccgcgccggc atccgcgact attgggcgac ggtgacggcc gatcaacgca
     3601 atatcgactt caagtcggaa gtcgtcgccg tcaacggcca aaccggcgtt gcgcgttggt
     3661 cggcgagcct gacgtcggcc tcgagcggcg cgcgcgtcga gctcgacggc gtattcattc
     3721 tgcagttcga ccggaacggc ctgtgcagtg agctgcgcga gtggtggcac gttcgcatgt
     3781 gaggacgcgg tggtaacaaa tcacagcgca cgtgccgcgt cggtggtata gtgctgcgcc
     3841 atctgtagcg cacattcggt ctgcgctcga cgcgacacag ccaattccgg ccgtgaccgc
     3901 ttctcaaaat ccgatcccct tggaccgttc ctcggccgcc tcgtgcgcca gggacagggc
     3961 gatcgcggag cttatttaca atgtcttttg attctttggg ccttgcgccc gcgctgctgc
     4021 gtgccgttca agaacagggc tacacgcagc ccaccccgat tcaaggccgc gtcatacccg
     4081 tcgtgctcga agggc
//
LOCUS       DATLEE010000430         4537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6202978, whole genome shotgun sequence.
ACCESSION   DATLEE010000430 DATLEE010000000
VERSION     DATLEE010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6202978"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VM692_09205"
     CDS             complement(<1..1199)
                     /locus_tag="VM692_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HUQ52387.1"
                     /translation="MNTALQGYAGRLETLALEHGLDYYPVQFEEVPSSFMMEIAVYGL
                     PVRMPHWSFGVRYIHQLVQHRMGHSRLFEVVFPGNPGRAYLAGNNSLQENTLVTAHVL
                     GHADFAKNNALFKRSQEQVGYRIVEQAAAHARQIGAAVEQYGQTRVEQVLDVALALEA
                     HIDPFQSLRRDRYPQYVEGPRAPHESGFQKRFDNLPGNATAADAPLERARAAIPPVPE
                     RDLLWFIAEYAPELEEWERDIFRTVREESFYFHPVFACQIMNEGWASYWHARLLREAD
                     FLPHNEYLDALKTHSDVVRPHAAEQQASLVTNPYHLGFSIWESIVAEHGIERAFQIRA
                     EDDDFSFIRNHLTQDLADELKLFRYERAADGSVKVLDHDIDALRETVLAPKYNFGAPA
                     VLSKHLGT"
     gene            complement(1201..2364)
                     /locus_tag="VM692_09210"
     CDS             complement(1201..2364)
                     /locus_tag="VM692_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HUQ52388.1"
                     /translation="MTGSSGFVDPSGSGRQWYDLFSRGARDWLRHNDKVRSAVRDKLP
                     ELIAGSDLLPSADRTVQVPVHFLEHARLRLREAEQAEGVGQGAGKPGDQLVKPSDGEL
                     AKGAGGNDEGGYQFILELKIDDIVDWLWEELKLPNLKVKAGAVQYDDYTREGWSRRGV
                     RSRLDRRRSLKEALKRRSVQSDEAPPFTNDDLRYRQLAMRKRPATEAVVYFAMDVSSS
                     MTENDRKLAKSFFFWVVQGLRRQYTHLEAVFVAHTVEAWEFQEAEFFQVSGSGGTVAS
                     TAFRKVLEIATSRYDPSRFNTYVFYASDGANFHNDRDAAQAALTELGNLANYVGYVET
                     ASIAQQALQSETALLFERLEAETQAAGKHALSSPESVWDAIRAFFSGRADVEV"
     gene            complement(2397..4394)
                     /locus_tag="VM692_09215"
     CDS             complement(2397..4394)
                     /locus_tag="VM692_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HUQ52389.1"
                     /translation="MTTLSESQVHWEIAGPRPAAPRGTFIDDLAVYATQHQAQHWRGT
                     FAEFLQDILPKDPSKLTRTSHQYVYDMLCAFRDAPLPEGAVAPDKAPTTTALFAGELF
                     GIDSALERVIDYFKAAAAGSDVGRRLLLLLGPPSGGKSSLVILLKRGLEEYSHTDDGA
                     LYALQGSPLHESPLNLIPASMRPRFRATYGVDIQGELSPYARARLDDEFRGDFMNFPV
                     ERIFLSEAARVGVGTYAPHDPTTADIADLVGSVDLSKVADIGDEGDPRAWSWSGAVYA
                     ASRGVLEMIEILKVKREFLYLLLTLTQEKNVKVSRFPLIHLDETILAHTNLAEFNKFL
                     QEKENEALLDRMVIVKVPYTLSFRDEARIYRKLVSSAPAFRKVHFDPHVLDLAAVFSI
                     LTRLQKPQREGLDLTKKLKLYANEDVEGFTAADVPRIHTESTDEGLTGVSPRFVINAI
                     SNAITRNNVASLTSMDMLLALKDAIETDARMDAGRKKQWIEFLVLARKDFYNRWVKED
                     VHRALFASFQEEAQQLLEKYLDEIEASLDKREVEDPITGDSRPPDERFLRSVEEKIKI
                     SESGKLSFRQEVVRKAMVAFKAGEKFTLDNHSRLHEAIEQYLFEERRDVLRLVTSKAR
                     PDDEAEQKISAVQERLILEYGYDEHSAKEALNYVTTLLSQE"
BASE COUNT          774 a         1398 c         1466 g          899 t
ORIGIN      
        1 gttccgaggt gcttcgagag caccgccggc gcgccgaaat tgtatttcgg cgcgagcacc
       61 gtctcgcgca gcgcatcgat gtcgtgatcg agcactttga cgctgccgtc ggcggcgcgc
      121 tcgtagcgaa atagtttcag ctcgtcggcg agatcctgcg tcaggtggtt gcgaatgaag
      181 ctgaagtcgt cgtcctcggc gcgaatttgg aatgcccgtt cgatgccatg ctcggccacg
      241 atcgattccc agatcgagaa cccgaggtgg taaggattcg tgaccaacga cgcctgctgc
      301 tcggccgcgt ggggacgcac gacgtcggag tgggttttca gcgcatcgag gtactcgttg
      361 tgcggcagga aatcggcctc gcgtagcagg cgcgcgtgcc aatacgacgc ccagccttcg
      421 ttcatgattt ggcaggcgaa gacgggatgg aagtagaacg actcctcgcg cacggtgcgg
      481 aagatgtcgc gctcccattc ctcgagctcg ggtgcgtact ccgcgatgaa ccacaacaga
      541 tcgcgctcgg gcacaggcgg gatcgcggcg cgcgcgcgct cgagcggggc atccgccgcc
      601 gtggcgttgc ccggcaagtt gtcgaaccgc ttctggaaac cgctctcatg cggcgcccgc
      661 ggtccttcga cgtactgcgg atagcggtcg cggcgcaggc tttggaaggg atcgatgtgc
      721 gcctcgagcg ccaatgcgac atcgagcact tgctcgacgc gtgtctggcc gtactgctcg
      781 acggcggcac cgatctggcg cgcatgcgcg gccgcttgct cgacgatgcg atagccgacc
      841 tgctcttggc tgcgcttgaa caacgcgttg ttcttggcga agtctgcgtg cccgaggacg
      901 tgcgccgtga ccagcgtgtt ctcttgcagc gagttgttgc ccgccaagta ggcgcgcccg
      961 ggattgcccg ggaacacgac ttcgaacagg cgcgaatggc ccatgcggtg ctgcacgagc
     1021 tgatggatgt aacggacgcc gaacgaccag tgcggcatgc ggacgggtaa cccgtacacc
     1081 gcaatctcca tcatgaagct cgatggcact tcctcgaact gcacggggta gtagtcgagg
     1141 ccgtgctcca gcgcgagcgt ctcgagacgg ccggcgtaac cttgcagcgc ggtgttcatg
     1201 ctagacctcg acgtcggcgc ggccgctgaa gaacgcgcgt atcgcgtccc agaccgattc
     1261 cggcgacgac agcgcgtgct tgcccgcggc ttgcgtttcg gcttcgaggc gctcgaacag
     1321 cagcgccgtc tcggactgca gcgcttgctg cgcgatcgag gcggtctcga cgtagccgac
     1381 gtaattcgcc aggttgccga gctccgtcaa cgcggcttgc gccgcgtcgc ggtcgttgtg
     1441 aaagttcgca ccgtccgagg cgtagaagac gtaggtgttg aagcggctcg ggtcgtagcg
     1501 gctggtggcg atctcgagca ccttgcggaa cgccgtcgag gccacggtgc cgccgctgcc
     1561 gctgacttgg aagaactcgg cttcctggaa ttcccacgcc tcgacggtgt gcgcgacgaa
     1621 cacggcttct agatgtgtgt actggcgccg caagccttgc acgacccaga agaagaatga
     1681 cttcgcgagc ttgcggtcgt tctccgtcat gctcgaggag acgtccatcg cgaaatagac
     1741 gacggcttcc gtcgctggcc gcttgcgcat cgcaagctgg cggtagcgca ggtcgtcgtt
     1801 cgtgaacggc ggcgcttcgt cgctctgcac cgatcgacgt ttcaacgctt ccttcagtga
     1861 gcggcgacga tcgagccgcg agcgcacgcc gcggcggctc caaccctcgc gcgtgtagtc
     1921 gtcgtactgc acggcgccgg ctttgacttt gagattcggt agcttcagct cctcccaaag
     1981 ccaatcgacg atgtcgtcga tcttgagctc gaggatgaat tggtaaccgc cctcgtcgtt
     2041 gccgccggca cctttcgcga gctcgccatc actaggcttg acgagctggt cgccgggctt
     2101 gccggcacct tggccgacgc cttcggcctg ctcggcctcg cgcaggcgca accttgcatg
     2161 ctcgagaaaa tggaccggca cctggacggt gcggtcggcg ctcggcaaca aatcgctgcc
     2221 ggcgatgagc tcgggaagtt tgtcgcggac cgctgagcgc accttgtcgt tgtgccgcag
     2281 ccagtcgcgg gcgccgcgcg agaacaggtc gtaccactgc cgcccggagc ccgaaggatc
     2341 cacgaaaccg gacgaccctg tcatgtttac gtgccctcct ttgcctctgt ctcgccctac
     2401 tcctgcgaca acaatgtcgt gacgtaattg agcgcttcct tggcgctgtg ctcgtcatag
     2461 ccgtactcga gaatcaaccg ctcttggacg gccgagatct tctgctccgc ctcatcgtcg
     2521 ggccgtgcct tcgaggtcac gagccgcagc acgtcacgcc gctcctcgaa caagtactgc
     2581 tcgatcgctt cgtgcaggcg cgagtgattg tcgagcgtga atttctcgcc ggccttaaat
     2641 gcaaccatcg ccttgcgcac gacctcttgg cggaacgaga gcttgccgga ctccgagatc
     2701 ttgatcttct cctcgacgga gcgcaggaat cgctcgtcgg gcgggcgcga gtcgccggtg
     2761 atcggatcct cgacttcgcg cttgtcgagc gaagcctcga tctcgtcgag gtacttctcg
     2821 agcagctgct gagcttcctc ttggaacgag gcgaacagcg cgcgatgcac gtcctcctta
     2881 acccagcggt tgtaaaagtc cttgcgcgcg agcacgagga actcgatcca ttgtttcttg
     2941 cggcccgcgt ccatgcgcgc gtcggtctcg atggcgtcct tgagagccag cagcatgtcc
     3001 atgctcgtga ggctcgcgac gttgttgcgc gtgatcgcgt tgctgatcgc gttgatgacg
     3061 aaccgcgggc tcacgcccgt gaggccttcg tcggtcgact cggtgtggat gcgcggcacg
     3121 tcagccgccg tgaagccttc gacatcctcg ttggcataga gcttgagctt tttcgtgagg
     3181 tcgagccctt cgcgttgcgg tttctgcagc cgcgtcagga tcgagaacac ggccgcaagg
     3241 tcgagcacgt gcgggtcgaa atgcaccttg cggaaagccg gggcgctcga cacgagcttg
     3301 cgataaatgc gcgcttcgtc gcggaacgac agcgtgtacg gcaccttgac gatcaccatg
     3361 cgatcgagca gcgcctcgtt ctccttttcc tggaggaact tattgaactc cgcgagattg
     3421 gtgtgcgcga gaatcgtctc gtcgagatga atcagcggga agcgcgaaac cttgacgttc
     3481 ttctcttgcg tcagcgtcaa gagcaaatac aggaattcgc gtttgacctt gagtatctcg
     3541 atcatctcga gcacgccgcg gctcgccgcg tagacggcgc cggaccacga ccacgcgcgc
     3601 ggatcgcctt cgtcgccgat gtcggcgacc ttggacaagt cgacggagcc gacgaggtcg
     3661 gcgatgtcgg ccgtcgtcgg gtcgtgcggt gcgtacgtgc cgacgccgac gcgagcggct
     3721 tcagacagga agatccgctc gacggggaag ttcatgaagt cgccgcggaa ttcgtcgtcg
     3781 aggcgcgcgc gagcataggg cgacagctcg ccctggatgt cgactccata ggtggcacgg
     3841 aaccgcggcc gcatgctagc cgggatcaga ttgagcggcg actcgtgcag cggcgatcct
     3901 tgcaacgcgt acagcgcgcc gtcgtcggtg tggctgtact cttcgaggcc gcgcttcagc
     3961 aggatgacga gggaagactt gccgcccgag ggcggaccca acagcagcaa cagtcgccgg
     4021 ccgacgtcgg agcccgccgc ggccgctttg aagtaatcga tgactcgctc gagcgcgcta
     4081 tcgatgccga atagctcgcc tgcgaacaat gctgtcgtcg tcggcgcctt gtcgggcgcg
     4141 acggcgcctt cgggaagcgg cgcatcgcgg aacgcgcaga gcatgtcgta aacgtactgg
     4201 tgcgacgtgc gcgtcagctt ggaagggtct ttcggaagga tgtcctgcag gaattctgca
     4261 aaggtgccgc gccagtgctg tgcttgatgc tgcgtggcat acacggccag atcgtcgatg
     4321 aaagtccccc ggggggctgc tggccgcgga ccggcaatct cccagtggac ttgagactcg
     4381 gatagcgtcg tcatcaacac tccctacctt gatgttttgc gatgccaggc gctggcttcg
     4441 ttcttgttgt tattggattg tcagacggcc ctccacactt ggttaccgat gagatttaga
     4501 cgaagtaact taatcggcgt tccgttaaat cgctgtc
//
LOCUS       DATLEE010000431         2142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10681290, whole genome shotgun sequence.
ACCESSION   DATLEE010000431 DATLEE010000000
VERSION     DATLEE010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10681290"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VM692_09220"
     CDS             complement(<1..599)
                     /locus_tag="VM692_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52390.1"
                     /translation="MPDCRRVLCLLAALTGAGTAFAADPEWIKLQAPGFGVISQLDED
                     DTRRWAVEFDQFIGAMHSLYNVEEVELPPLTIVLFKQYKDFAPYRVVTQSGQARIAGF
                     FGRKGDWSVIGLSGAVRDAATRHTIYHEAVHWFASASTTRPPLWFSEGLAEVLSTFRS
                     VDGKGRWGEAIEDNVAYLTYTGVMPIEELLVASQDEALHG"
     gene            complement(685..1407)
                     /locus_tag="VM692_09225"
     CDS             complement(685..1407)
                     /locus_tag="VM692_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HUQ52391.1"
                     /translation="MGRIFEVRKHTMFARWDRMAKQFSRISKEIIVAVKAGGADPHSN
                     PALRRAVQNARQINMPKDKVEAAIKRAAGKDATNYEQIIYEGYAPHGVAVVVECATDN
                     PTRTVASVRMIFSKNGGNLGSTGSVAFQFRKMGVFRLNPEGIDHDDLELYLIDYGLDE
                     MGDSTGDKGEPQIVARCPFADFGRMQEALEKRGITPLLATHEYICTAPVELPEAKANE
                     ALAMIDKLEQDDDVQNVYHSLI"
     gene            1438..>2142
                     /locus_tag="VM692_09230"
     CDS             1438..>2142
                     /locus_tag="VM692_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52392.1"
                     /translation="MTIRRAQLVAWFDAQAVATVDELVPLEYSRAGGQGLPEHVRAAS
                     ALRRFNDRLVIVQDDVNALAVRDASGAVQPVLLPPDAEGRRMFDDAHGNKRDKLDLEA
                     CVTLPDGRLVAFGSGSLPKRERLVVWRGGRESPLVVDSQSFYRDLRAAITRGEALLNI
                     EGAVVCANKLRLFHRGNDKRAANRTSANAIAEVDCGEFAAWLDRKAGASPSVASVTTV
                     ELGDIDGIPFGFTDAVA"
BASE COUNT          351 a          701 c          703 g          387 t
ORIGIN      
        1 ccatgcagcg cttcatcctg cgaagcgacg agcagctcct cgattggcat gacaccggtg
       61 taggtaaggt acgccacgtt gtcttcaatc gcttcgcccc aacggccctt gccgtcgacg
      121 ctgcggaacg tcgacaacac ctcagcgagg ccttcggaaa accacaacgg cggccgcgtc
      181 gtcgatgcgc tcgcgaacca atgcaccgcc tcgtgataga tcgtgtggcg cgtcgcggca
      241 tcgcggaccg cgccgctgag cccgatgacg ctccagtcgc ctttgcgccc gaagaagccc
      301 gcgatgcgtg cctgaccgga ctgcgtgacg actcggtacg gagcaaagtc tttatattgt
      361 ttgaacagga cgatcgtcag cggcggcagc tcgacctctt cgacgttgta gagcgaatgc
      421 atcgcgccga tgaactgatc gaactcgacg gcccaccggc gtgtatcgtc ctcgtcgagc
      481 tgcgagatga cgccgaaccc cggcgcttgc agcttgatcc actcggggtc cgcggcgaac
      541 gccgtaccgg cgcccgtgag tgcggcgagc aggcacaaga cgcgtcgaca atcgggcatc
      601 gccgggctcc gcagccatga cgcgcgaatc gtactaggga ctccgtggca gttccacggc
      661 tgggagcttc cggaaggcgc ttcgctagat cagggagtgg tagacgttct ggacgtcgtc
      721 gtcctgctcg agcttgtcga tcatggcgag cgcttcgttg gccttggctt ccggcagctc
      781 gacgggcgcc gtgcagatgt attcgtgtgt tgccaaaagc ggcgtgatgc cgcgcttttc
      841 gagcgcttcc tgcatcctgc cgaagtcggc gaacgggcag cgcgcgacga tctgcggctc
      901 gcctttgtcg ccggtgctgt cgcccatctc gtcgaggccg tagtcgatca gatagagctc
      961 gaggtcgtcg tgatcgatac cctcgggatt caggcggaag acgcccatct tgcggaactg
     1021 aaacgcgacg ctgcccgtgc tgcccaggtt gccgccgttc tttgaaaaga tcatgcggac
     1081 cgacgccacc gtgcgcgtcg ggttgtccgt cgcacactcg accacgaccg cgaccccgtg
     1141 cggcgcatag ccttcgtaga tgatctgctc gtagttcgtc gcgtccttgc cggcggcgcg
     1201 cttgatcgcg gcctcgacct tgtccttcgg catgttgatc tgccgagcgt tctgcacggc
     1261 gcggcgcaac gcgggattgc tgtgcggatc ggcgccgccg gccttgacgg ccacgatgat
     1321 ttccttggaa atgcgcgaga actgcttggc catgcggtcc cagcgcgcaa acatcgtgtg
     1381 ctttcgaact tcgaagatac gtcccatggg cgcaaagttt ggcagatggc gagcgcggtg
     1441 accattcgcc gcgcgcagct cgtggcgtgg ttcgacgcgc aagccgtcgc gaccgtcgac
     1501 gagctcgtgc cgctcgaata ctcgcgcgcc ggcggccaag gtctgcccga gcacgtgcgc
     1561 gcggcttcgg cactgcgccg tttcaacgac cggctcgtca tcgtgcaaga cgacgtcaac
     1621 gcgctggcgg tgcgcgatgc gagcggcgcc gtgcagcccg tcttgctgcc gccggacgcg
     1681 gaggggcggc gcatgttcga cgacgcccac gggaacaaac gcgacaagct cgacctcgaa
     1741 gcctgcgtga ccttacccga cggccgcctc gtggcattcg gctcgggctc gctgcccaaa
     1801 cgcgagcggc tcgtcgtgtg gcgaggcgga agggagtcgc ctttggtcgt cgacagccag
     1861 tcgttctatc gcgacctgcg cgcggcgatc acgcgcggcg aggcgctgct gaacatcgaa
     1921 ggcgccgtcg tctgtgccaa caagctccgg ctttttcatc gcggcaacga caagcgcgcc
     1981 gccaaccgta cgagcgcgaa cgcgatcgcc gaagtcgact gcggcgagtt cgccgcttgg
     2041 ctcgatcgga aagccggcgc gagcccgagc gtcgcgagcg tgacgacggt cgagctcggc
     2101 gacatcgacg gtattccgtt cggcttcacg gacgccgtcg cg
//
LOCUS       DATLEE010000432         2972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3237286, whole genome shotgun sequence.
ACCESSION   DATLEE010000432 DATLEE010000000
VERSION     DATLEE010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3237286"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VM692_09235"
     CDS             <1..231
                     /locus_tag="VM692_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52393.1"
                     /translation="MSSKLSWVNIVQYDNASRSVGINSRIHWIPEAGRELFIVLNHNL
                     EDPDELPFQRIDDDFHSATADLTIKYRHTFRY"
     gene            complement(250..1476)
                     /locus_tag="VM692_09240"
     CDS             complement(250..1476)
                     /locus_tag="VM692_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HUQ52394.1"
                     /translation="MAEDANAALLETLQRLLEIPAADLRAALSEACDAVAAAVHADKV
                     DAFLYDATRDSLVALGTSAQPLSAKQHRHGLDVLQIANGGRTVHVYETGETFVTGHLE
                     DDEQEVRGIRETLAVRSQIGVPLQVAGKRRGVLMIASLQPDAFSRQDVGFAQAVTRWI
                     GSVAHRAELVEEIERNAVEQGRRVVADELITMLAHDLRNFISPMDFRLRAIRRRAELE
                     GRAAEVGDADAALKSVARLGGLIADILDAARIDQGVLEVETRPIALDQLVDEVINALA
                     TAEHPIVCKSAEQLTVDGDPRRIRQCLENVLANAVRHSPSEAPVTIATRRSSQGNGPI
                     ARIEIIDEGPGIAPELLPHVFERFVTGKGRKDGLGLGLYLAKRIATLHNGDLCVESKP
                     GKGARFTLTLPCYIES"
     gene            1584..2264
                     /locus_tag="VM692_09245"
     CDS             1584..2264
                     /locus_tag="VM692_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUQ52395.1"
                     /translation="MNTPAITSPLGDAPDNELARRVAAGEPAAFEALMRRHNRKLFRT
                     ARAILRDDAEAEDALQDAYIQALRAIGGFRGEAKLSTWLAKVVANEALMRRRKRVRRA
                     EIVPLQPGTTESELNEIPDGNMDEQPERSAQRSEMRRLLEAQIDSLPDDFRAVFVLRA
                     VEEMSVEETAEALGIPQATVRTRLFRARSLLREALAAKIDLACEEAFSFAGDRCDRIV
                     AKVMERMR"
     gene            2261..2836
                     /gene="folE"
                     /locus_tag="VM692_09250"
     CDS             2261..2836
                     /gene="folE"
                     /locus_tag="VM692_09250"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HUQ52396.1"
                     /translation="MTALLKSVHVHDAAPDPIAAHVSAIIEDLGEDVARGGLAATPAR
                     YAKALRYLTSGYAGDVESAVGNGLFAAEGGSPVLLRDVQFYSLCEHHLLPFHGRIHVG
                     YLPDRRIIGLSKIPRIVDLFARRFQVQERLTEQVADALVQTLEPKGVIVVSEAQHLCV
                     AMRGVQEHGASTTVCATRGDGERLLTLLQRR"
BASE COUNT          511 a          995 c          971 g          495 t
ORIGIN      
        1 atgtcgtcga agctgtcgtg ggtaaacatc gtgcaatacg acaacgcgtc gagaagcgtc
       61 ggcatcaata gccgaatcca ctggattccc gaggccggcc gcgagctgtt catcgtcctc
      121 aatcacaatc tcgaggaccc cgacgagctg ccgttccagc gcatcgacga cgacttccac
      181 tccgcgaccg cggacctcac gatcaagtac cggcacacgt tccgctacta gtggcgttgc
      241 cgcggtaagc taactctcga tgtagcaggg cagcgtcaac gtgaagcggg cgcctttgcc
      301 cggcttcgac tcgacgcaga ggtcgccgtt gtgcagcgtc gcgatgcgct tcgcgagata
      361 caggccgagc ccgagcccgt ctttgcgccc tttgccggtc acgaagcgct cgaaaacatg
      421 cggcagcagc tccggcgcaa tgccgggccc ctcgtcgatg atctcgatgc gtgcgatggg
      481 gccgttgccc tggctcgagc gccgtgtcgc gatcgtcacg ggcgcttcgc tcggcgagtg
      541 cctcacggcg ttcgccagga cgttctcgag gcattgtcgg atgcggcgcg ggtcgccgtc
      601 gacggtcagc tgctccgccg acttgcacac gattgggtgc tccgccgtcg ccagggcgtt
      661 gatgacttcg tcgacgagct gatcgagcgc gatcggccga gtttcgacct cgagcacacc
      721 ctggtcgatg cgtgccgcat cgagaatgtc tgcgatcagc ccgccgagcc gcgcgacgct
      781 cttcagagcc gcgtccgcgt cgccgacttc ggcagcgcgt ccttcgagct cggcgcggcg
      841 gcgtatcgcg cgcagcctga agtccatcgg cgagatgaaa ttgcgcagat cgtgcgccag
      901 catcgtgatc agctcgtcgg cgacgacgcg ccggccctgt tccacggcat tgcgctcgat
      961 ctcctcgacg agctcggcgc gatgcgccac actgccgatc cagcgcgtca cggcctgcgc
     1021 gaagccgacg tcttgccgcg agaaagcgtc gggttgcagc gacgcgatca tcagcacgcc
     1081 tcgccgtttg ccggcgacct gtagcgggac gccgatttgc gagcgaacgg cgagcgtctc
     1141 gcgtatgccg cgcacctcct gctcgtcatc ctcgagatgc cccgtcacga acgtctcgcc
     1201 ggtttcatag acgtggacgg tgcgcccgcc gtttgcaatc tgcagtacgt cgagaccatg
     1261 tcgatgctgc tttgccgaga gcggctgagc gctcgtgccg agcgcaacga gcgagtcgcg
     1321 cgtggcgtcg tagagaaacg cgtcgacttt gtcggcgtga acggccgccg cgacggcatc
     1381 gcaagcttcc gacagcgccg ctcgcaaatc ggcggccggg atctcgagca accgctgcaa
     1441 cgtttcgagc aacgcggcgt tggcgtcttc tgccatggca cctccggcag cggctctagg
     1501 gcgcgcgcca atggccatcc gcagcaaaaa cggtgccagc agcacgggaa cgcagcggcg
     1561 ggtcgagcca tccaattccc cgtatgaaca cacccgcaat cacgagccca ctgggcgacg
     1621 cgccggacaa cgagctcgcg cgacgcgtgg ccgcgggcga gcccgcggca ttcgaggcgc
     1681 tcatgcgccg gcacaaccgc aagctatttc gcacggcacg cgcgatcttg cgcgatgacg
     1741 ccgaagccga ggacgcgctg caagacgcgt acatccaggc cttgcgcgcg atcggcggtt
     1801 tccgcggcga agcgaagctg tcgacgtggc tcgctaaagt cgtcgcaaac gaggcgttga
     1861 tgcgccgccg caaacgcgtc cggcgcgccg agatcgtgcc gctgcagccc ggcacgacgg
     1921 agtccgagct caacgaaata ccggacggca acatggacga gcagccggaa cgttcggcgc
     1981 agcgcagcga gatgcgccgc ctcctcgaag cacaaatcga ctcgctgccc gacgactttc
     2041 gcgctgtgtt cgtgctgcgc gccgtcgagg agatgtctgt cgaggagacg gccgaagcgc
     2101 tcggcattcc gcaggccacg gttcgcacgc ggttgttccg cgctcgcagc ctgctgcgcg
     2161 aggcccttgc ggccaagatc gatctcgcct gcgaggaggc attcagcttc gccggcgacc
     2221 gttgcgaccg aatcgtagcc aaggtgatgg agaggatgag atgacagcgc tactgaaaag
     2281 cgtccacgtg cacgacgcag caccggatcc gatcgccgcg catgtgagcg caatcatcga
     2341 agatctcggc gaagacgtcg cgcgcggcgg cctcgctgct acgccggcgc gttatgcgaa
     2401 agcgctgcgc tatctaacga gcggttatgc cggcgacgtc gagtccgcgg tcggcaacgg
     2461 cctcttcgcg gccgaaggcg ggagccccgt gctgctgcgc gacgtgcagt tctactcgct
     2521 ctgcgagcac catctgctgc cgtttcacgg ccgtattcac gtcggctatc tgccggaccg
     2581 ccgcatcatc ggcttgtcga agatcccgcg catcgtcgat ttgttcgcac ggcgcttcca
     2641 ggtgcaggag cggctgaccg agcaagtcgc cgatgctctt gtgcagacgc tcgagccgaa
     2701 aggcgtgatc gtcgtcagcg aggcgcagca cctctgcgtg gccatgcgcg gcgtgcagga
     2761 gcacggcgcg tcgacgacgg tgtgcgcgac gcgcggcgac ggcgagcggc tattgacgtt
     2821 gctgcaacgc cgctgactcg cgctactctt cggcggccgc gaacgacgtc aagctttgcg
     2881 ggatgaccgc gtacgcataa ggtacgtaca gcccgagctc gatcgctccg agccggaacc
     2941 attcgacggg tcgatcgatg ccttgcacga cg
//
LOCUS       DATLEE010000433         8814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9935458, whole genome shotgun sequence.
ACCESSION   DATLEE010000433 DATLEE010000000
VERSION     DATLEE010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8814
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9935458"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            635..1735
                     /gene="ald"
                     /locus_tag="VM692_09255"
     CDS             635..1735
                     /gene="ald"
                     /locus_tag="VM692_09255"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HUQ52397.1"
                     /translation="MAIGVPKEIKQKEHRVALLPAAAWQLVQRGERVLIETGAGAGAG
                     YDDGEYRKAGCEILPDAARVFEAADLIVKVKEPQPREITMLGPKHTLFTYLHLAADRA
                     LTEGLMNSGCTAIAYETVEKNRRLPLLEPMSEIAGRMSVMVGAYFLAKHNGGRGVLLG
                     GVPGVLPGKVLIIGGGTAGMNAAQVATGVGADVTILEVDVERMRFLDITMHSAHTLYS
                     NPGALLETLPDVDLIIGAVLVPGAKAPKLITREMLHVMKAGTVFVDIAIDQGGCAETS
                     RPTTHDNPTFVEEDVIHYCVTNMPGAYARTATQALTNVTFPYVQWLAEGGVGGACAKH
                     PELASGVNIQSGRVTNRAVAAAHGLPFADVTV"
     gene            1754..2866
                     /locus_tag="VM692_09260"
     CDS             1754..2866
                     /locus_tag="VM692_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020030905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HUQ52398.1"
                     /translation="MARKRGMTIGIVGAGKIGATIATLLESCTFCDAVALADVRAELK
                     IGGLAKASATRLDAQQRTDLTAFIERCDAIVSAGPYLINREIVETCAQAGVAYFDLTE
                     DVATTKLVRQLARESEGVTFVPQCGLAPGAINIVAGSLTASLATVRSVEMRVGALPQF
                     ASNQMKYYLSWSTAGLINEYCQLGEALHEGRPIATLPLDGVERLTIDGVEYEAFNTSG
                     GVATMCETFAGKVGELNYKTLRYPGHRDLMKFLLQDLNLAHQRELLTQIFDQEVPLTQ
                     HDVVVFYVNAVGTSDGELVQRSYVKKIHGDNVEGRQLSAIQLTTAAGVVAVLELFARG
                     ALARSFVKQESITFEQFLSTRWGGRVYGGDREPAQR"
     gene            2863..4476
                     /locus_tag="VM692_09265"
     CDS             2863..4476
                     /locus_tag="VM692_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013138848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUQ52399.1"
                     /translation="MTAKSRSTSVKKVAAEVLGALQLGADNLGAFAGDWHGSGPALEK
                     RSPIDGSVLARVTTASPSEVAQAIGAAERAFRAWRNVPAPQRGELLRRYAEALRTHKS
                     DLGRLVTLETGKILAEGEGEVQEMIDICDFAVGLSRQLYGLTIASERPAHRLMEQWQP
                     LGVVAIVSAFNFPAAVWSWNSVLATVCGDATIWKPSEKTPLTAIACVKLAERVCRERN
                     VDPAIFSLVCGDGPTVGEPLIADARVALVSATGSTRMGRRVSEVVAQRLGRTLLELGG
                     NNAIIVAPSADLKLALRGVLFAAVGTAGQRCTTTRRLIVHESVYGEFTRMLKRAYAQV
                     AIGNPLEPTTLMGPLIDARAVASYESAIAAAKSQGGRVLYGDPWKSRKGDDPLKSPKG
                     GEPPSAEWPANGSYVSPCIIEADNAWEIVQQETFGPILYVMPYRSLEDAIALQNAVPQ
                     GLSSAIFTNDVREAELFTSVRGSDCGIANVNMGTSGAEIGGAFGGEKQTGGGRESGTD
                     SWRSYMRRQTVTVNYSTDLPLAQGVRFGE"
     gene            4476..5612
                     /locus_tag="VM692_09270"
     CDS             4476..5612
                     /locus_tag="VM692_09270"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HUQ52400.1"
                     /translation="MLLPLATCPSLHFGRRIVVACVGLLLCGVLAQRAFAHPAPFSYL
                     DLRLDPDGARGTLVIHDFDAAHELGLAEPRTLLEPSVAQQRAGELMRLIDERLLISQD
                     GEPATLQWRPGVQVLAEQQSLRFDFTLAPAGSAARVEIDTVLFPYDGTHQTFINLYEG
                     ARLERQAVLNASAHRFEHYVGTIQGRAAVVRRFVALGAEHILIGPDHLLFLLGLLLLG
                     GSLWRLATIVTSFTLGHTITLSLAALDVVRLSPALVEPAIALSIVVVGVDNLLVGRRG
                     GKAAASVDLRPWLAAAFGLIHGFGFAAVLREIGLPPGALGWSLAGFNVGVELGQLAAV
                     LVAALLLAGVRRRDVVLAERVALGGSVAVVAVGLYWFAARIGLA"
     gene            5640..6554
                     /locus_tag="VM692_09275"
     CDS             5640..6554
                     /locus_tag="VM692_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ52401.1"
                     /translation="MRRPFVLSLVIGGGLAAVAVFGQAPQQPAAPANAPQFPPVDSID
                     SLASNLYVVPGQGGNTAVFVRGDGVLLVDTKLANNGQAILDQVRKVTDKPITHIVNTH
                     THGDHTGSNMFFPANVEIVTQANTSANMAKMPAFQEAGGKVGMPDRTFTDKLTLFSGK
                     ESVDLYYFGAGHTNGDAFVVFRDARVMHAGDIFATKGLPLLDVNNGGSGVAIGDTLKK
                     AADGIRNVDRVIPGHSTTMTWQDFVDYGEFNRLFLEHARTSRAAGKTAEQAAADLKLP
                     AKFSGYNLTARGGPANNLGIIYTELGSR"
     gene            6569..7141
                     /locus_tag="VM692_09280"
     CDS             6569..7141
                     /locus_tag="VM692_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52402.1"
                     /translation="MSNGSRWPAVAVLCLAAVSACRQSPAGNAASPSTAPSPPVMTVN
                     QLMHGILFPAANTVFYAQADDPAAVPRDPMPSSSTNSLSGLYGGWQAVENSALVLVES
                     ADLMLTPGRMCENGEVVDVGNEDWIRFVADYREKSLAAYEAALTKNQDTMVDASGYLS
                     EACLACHRVYRREPTPGITTQRCIPGAPTR"
     gene            complement(7138..8175)
                     /locus_tag="VM692_09285"
     CDS             complement(7138..8175)
                     /locus_tag="VM692_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ52403.1"
                     /translation="MSTNRDDPRTATRRTVLAGAAGLAALGWRLPLSAQPPPAANRTF
                     PPTGTRLVMLGTRGGPGVSLDRGETASAVVVDGVPYLVDCGYGTMRALVASGLGYQAV
                     NTIFLTHLHDDHTADLAALLAHQWTGTKTAPTSVYGPYATESTVAGALAFFRANVEIR
                     TVDEGRTSKPEELFFGHDLDVGNDPVRVFADERVVVTAATNTHFPERFVAKMQHRSLG
                     YRFETSTRSICFSGDTAYSANIVKLARGADLFVCEVMDHRNYETNMARAKEEAAAGRP
                     ENVFRHVAETHVTPADAGRMAREAGVKTIVLNHQVRGQAANGFTISSFIDGVRAEFDG
                     EVIVGEDQQVI"
     gene            8313..>8814
                     /locus_tag="VM692_09290"
     CDS             8313..>8814
                     /locus_tag="VM692_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52404.1"
                     /translation="MNEDVIRIRGARQNNLRSVDLDLPLGELIVVTGVSGSGKSSLAF
                     DTVYSEGQRRYVETFSPYARQFLDRMDKPQVDRIEGIPPAIAIDQTNPVRTSRSTVGT
                     MTELNDHVKLLYARAARLYCRNCGRPVHRDDAESIYEDLAHEPALRAAGARALVTFTI
                     NVPENFS"
BASE COUNT         1433 a         2929 c         3033 g         1419 t
ORIGIN      
        1 gtactgacga ccgcgatgcg ccgcgcgttg acgagcgagc aggacgcgtt cggccaggcc
       61 gtttgggacc acacccacgg caagaacgcc ttcgagatca tcgagcgctc ggacgggcgc
      121 ttcgacatct cgagcggccc gtccgcctat ctcgccgaac ccgcgttctg gagcccgcac
      181 gaacgagcag ggctcgagaa gcatgcgcac ggccgcgtgc tcgacatcgg ctgcaacgcc
      241 gggcggcacg cgctgtactt gcaggaacgc gggctcgacg tggtgggcgt cgacgtgtcg
      301 ccgctggcaa tcgaagtggc gcgaaagcgc ggactgaaaa gcgtcttttt gctgtcgatc
      361 gacgagctca cgcccgagct cggcttgttc gacacgatcc tcatgctcgg caacaacttc
      421 gggctgttcg gcaatgccgc aagagcgaag cggttgttgc ggcgctgctc gacggcaccg
      481 gctggcgggt cgcgcagtac ttcgcggtgt cggtgctcgc gcggtctaca cggcgatcat
      541 cgaacggacc gactcacgat agttgaggct cgacggttgc gcagcgcgcc gccgggatca
      601 acaattcggc caggctccct accgacgttc gactatggcc atcggcgtcc ccaaggaaat
      661 caagcagaag gagcaccgcg tggcgttgct gccggcggct gcctggcagc tcgtgcaacg
      721 cggcgagcgc gtcctcatcg agacgggggc cggcgccggc gcgggctacg acgacggcga
      781 gtatcgcaaa gcgggttgcg agatcttgcc cgacgcggcg cgcgtgttcg aagcggcgga
      841 cctcatcgtc aaggtcaagg agccgcagcc gcgcgagatc acgatgctcg gcccgaagca
      901 cacgttgttc acgtatctgc acctcgcggc cgaccgcgcg ctgacggaag ggctcatgaa
      961 cagcggctgc acggcgatcg cctatgagac cgtcgagaag aaccgtcgtc tgccgctgct
     1021 cgagcccatg agcgagatcg cgggccgcat gagcgtcatg gtcggagcgt atttcctcgc
     1081 gaagcacaac ggcggccgcg gcgtgttgct cggcggcgtg ccgggcgtgc tgccgggcaa
     1141 ggtcctgatc atcggcggcg gcacggccgg catgaacgcg gcgcaggtcg cgaccggcgt
     1201 cggcgccgac gtcacgatcc tcgaggtcga cgtcgagcgc atgcggttcc tcgacatcac
     1261 gatgcactcg gcgcacacgc tgtactcgaa ccccggcgcg ttgctcgaga cgttgcccga
     1321 cgtcgacctc atcatcggcg ccgtgctggt ccccggggcc aaggcgccga agctcatcac
     1381 gcgcgagatg ctgcacgtca tgaaggccgg cacggtgttc gtcgacatcg ccatcgatca
     1441 aggcggctgc gctgagacgt cgcggccgac gacgcacgac aacccgacgt tcgtcgagga
     1501 ggacgtcatc cattactgcg tgacgaacat gccgggcgcg tacgcgcgca cggcgacgca
     1561 agcgctgacg aacgtgacgt tcccctacgt gcagtggctg gccgaaggcg gcgtcggcgg
     1621 cgcttgcgcc aagcatcccg agctcgcgag cggcgtcaat atacagagcg gccgcgtcac
     1681 gaatcgcgcc gtggctgccg cgcacggcct gccgttcgcc gacgtgacgg tctgacgccg
     1741 gtcgaggagt ggggtggcac gcaaacgcgg catgaccatc ggcatcgtcg gcgccggcaa
     1801 gatcggcgcg acgattgcga cgctgctcga atcgtgcacg ttttgcgacg ccgtggccct
     1861 cgccgacgtc cgagccgagc tcaagatcgg cgggctcgcg aaggcgtcgg caacgagact
     1921 cgacgctcag cagcgcacgg acttaaccgc gttcatcgaa cgctgcgacg ccatcgtcag
     1981 cgcgggcccg tatctcatca atcgcgagat cgtcgagacg tgcgcgcagg ccggcgtcgc
     2041 gtacttcgac ctcacggagg atgtcgcgac gacgaagctc gtgcggcagc ttgcgcgtga
     2101 gtccgagggc gtcacgttcg tgccgcagtg cggcctcgcg ccgggtgcca tcaacatcgt
     2161 cgccggctcg ctgacggcgt cgctcgcgac cgtccgctcg gtcgagatgc gcgtcggtgc
     2221 gctgccgcaa ttcgcgagca accagatgaa gtattacctc agctggagca cggccgggtt
     2281 gatcaacgag tactgtcagc tcggcgaggc gctgcacgag ggacggccga tcgcgactct
     2341 gccgctcgac ggcgtcgagc ggctcacgat cgacggcgtg gaatacgagg cgttcaacac
     2401 ctcgggcggc gtggcgacga tgtgcgagac gttcgccggc aaggtcggcg agctgaatta
     2461 caagacgttg cgctaccccg gccaccgcga cctgatgaaa tttctcctac aggacttgaa
     2521 cctcgcgcat cagcgcgagc tgctgacgca gatcttcgat caagaggtgc cgttgacgca
     2581 gcacgacgtc gtcgtcttct atgtcaacgc cgtcggcacg agcgacggcg agctcgtgca
     2641 gcgcagctac gtcaagaaga tccatggcga caacgtcgag gggcggcagc tgtcggcgat
     2701 tcaattgacg accgcggccg gcgtcgtcgc ggtgctcgag ctgtttgcgc gcggcgcgct
     2761 cgcgaggtcg ttcgtcaaac aggagtcgat cacgttcgag cagttccttt cgacacgctg
     2821 gggcggccgc gtctacgggg gcgaccgcga gccggcgcaa cgatgacggc gaaatcgcgc
     2881 tcgacgtcgg tcaagaaagt cgcggcagag gtcctcggcg cgcttcagct cggcgccgac
     2941 aacctcggcg cgttcgccgg cgattggcac ggctccggtc ccgcgctcga gaagcgctcg
     3001 ccgatcgacg gcagcgtgct ggcccgcgtg acgacggcgt caccgagcga ggtcgcgcag
     3061 gccatcggcg ctgcggagcg cgcgtttcgc gcctggcgca acgtgccggc gccgcagcgc
     3121 ggcgagctgc tgcgccgcta cgccgaggcg ctgcggacgc acaaaagcga cctcggccgc
     3181 ctcgtgacgc tcgagaccgg caagatcctc gccgagggcg agggcgaagt gcaggagatg
     3241 atcgacatct gcgatttcgc cgtcggcctc tcgcgccagc tgtacgggct cacgatcgcc
     3301 agcgagcggc cggcgcatcg cttgatggag cagtggcagc cgctcggcgt cgttgcgatc
     3361 gtctcggcgt tcaatttccc ggccgcggtt tggtcgtgga acagcgtgct ggccacggtc
     3421 tgcggcgatg cgacgatctg gaagccgtcg gaaaagacgc cgctgacggc gatcgcctgc
     3481 gtcaagctcg ccgagcgcgt ctgccgcgag cgcaacgtcg accccgcgat cttctcgctc
     3541 gtctgcggcg acggcccgac ggtcggcgag ccgctgatcg cggacgcgcg cgtggcgctc
     3601 gtgtcggcga ccggctcgac gcgcatgggt cgccgcgtca gcgaagtcgt cgcgcagcgg
     3661 ctcggccgca cgctgctcga gctcggcggc aacaacgcga tcatcgtggc gccgtcggcg
     3721 gacttgaagc tggcgctgcg cggcgtgctg ttcgcggccg tcggcacggc ggggcagcgc
     3781 tgcacgacga cgcggcgcct catcgttcac gagtccgtct acggcgaatt cactcggatg
     3841 ctgaagcgcg cctatgcgca ggtggcgatc ggcaatccgc tcgagccgac gacgctgatg
     3901 ggcccgctga tcgacgcgcg cgccgtcgcg agctacgaga gcgcgatcgc cgcggcgaaa
     3961 agccaaggcg gcagggtgct ttacggcgac ccttggaagt cccggaaggg cgacgaccct
     4021 ttgaagtccc cgaagggggg cgagccgccg agcgccgagt ggccggcgaa cggctcgtac
     4081 gtgagcccgt gcatcatcga ggccgacaac gcctgggaga tcgtgcagca ggagacgttc
     4141 ggcccgatcc tctacgtaat gccgtaccgt tcgctcgagg acgcgatcgc gttgcaaaac
     4201 gccgtgccgc agggcttaag ctccgcgatc ttcacgaacg acgtgcgcga ggccgagctg
     4261 ttcacgagcg tgcgcggcag cgattgcggc atcgccaacg tcaacatggg cacgagcggc
     4321 gccgagatcg gcggcgcgtt cggcggcgag aagcaaaccg gaggcgggcg cgagagcggc
     4381 accgattcat ggcgcagcta catgcgccgc caaacggtca cggtcaatta ttcgacggac
     4441 ttgccgttgg cacagggagt ccgcttcggc gagtgatgct gttgccgctc gccacctgcc
     4501 cgtcgctgca tttcggccgc cggatcgtcg tcgcctgcgt cggcctcctg ctctgtggcg
     4561 tcctggccca gcgcgccttc gcgcacccgg cgccgttcag ctacctcgat ctgcgcctcg
     4621 acccggacgg cgcgcgcggc acgctcgtga tccacgactt cgacgcggcg cacgagcttg
     4681 ggctagccga gccgcgaacg ctgctcgagc cgagcgtcgc gcagcagcgc gccggcgagc
     4741 tcatgcggct catcgacgag cggctgctca tcagccaaga cggtgagccc gcgacgctgc
     4801 aatggcgacc cggcgtgcag gtgttagccg agcagcagag cctgcgcttc gatttcacgc
     4861 tcgcgccggc gggttcggcg gcccgcgtcg agatcgacac cgtgctcttt ccgtacgacg
     4921 gcacgcatca aacgttcatc aacctctacg agggtgctcg gctcgagcgc caggcggtgc
     4981 tgaacgcgag cgcgcatcgg ttcgagcact acgtcggcac gatccagggc cgcgccgccg
     5041 tcgtgcgcag gttcgtggcg ttgggcgccg agcacattct catcggtccg gaccatctgc
     5101 tgttcctgct cggactgttg ctgctgggcg gctcgctgtg gcggctcgcg acgatcgtca
     5161 cgtccttcac gctggggcac acgatcacgc tgtcgctggc ggcgctcgat gtcgtccgcc
     5221 tatcgccggc gctcgtcgag cccgcgatcg ccttgagcat cgtcgtcgtc ggcgtcgata
     5281 acctgctcgt cggccggcgc ggcggcaagg cggcggcgag cgtcgatctg cggccctggc
     5341 ttgccgcggc gtttgggctg atccatggct tcggcttcgc agcggtgctg cgcgagatcg
     5401 ggttgccgcc gggcgccctc ggctggtcgt tggccgggtt caacgtcggc gtcgagctcg
     5461 ggcagttggc cgccgtgctc gtggctgccc tgctgctggc aggcgtgagg cgacgcgacg
     5521 tagtattggc cgagcgcgtc gcgcttgggg ggtccgtggc agtggtggcg gtgggactct
     5581 actggttcgc ggcgcgcatt gggttagcct aggcgtccag agtggggaga aagtacaaca
     5641 tgcgacgtcc attcgttctt agcctcgtca tcggcggcgg tctcgcggcg gtagccgtgt
     5701 ttgggcaggc tccgcagcaa ccggctgcgc cggccaatgc gccacagttt ccgcccgtcg
     5761 attcgatcga ctcgctcgcc agcaatctgt acgtcgtgcc gggtcaaggc ggcaacacgg
     5821 cggtgttcgt gcgcggcgac ggcgtgttgc tcgtcgatac caagctcgcg aacaacggtc
     5881 aggcgatcct cgatcaagtg cgcaaggtca cggacaaacc gatcacgcac atcgtcaaca
     5941 cgcacacgca cggcgatcac acgggcagca acatgttctt cccggcgaac gtcgagatcg
     6001 tgacgcaggc gaacacgtcg gcgaacatgg ccaagatgcc ggcgttccaa gaggccggcg
     6061 gcaaggtcgg aatgccggat cgcacgttca cggacaagtt gacgctcttc tcgggaaaag
     6121 agtccgtcga cctctactac ttcggcgccg gccacacgaa cggcgacgcg tttgtcgtct
     6181 tccgcgacgc gcgcgtcatg cacgccggcg acatctttgc gaccaagggc ctgccgctgc
     6241 tcgacgtcaa caacggcggc agcggcgttg cgatcggcga cacgctgaaa aaggccgcgg
     6301 acggcatccg caacgtcgac cgcgtgatcc cagggcactc gactacgatg acgtggcaag
     6361 acttcgtcga ctacggcgag ttcaaccggt tgttcctgga gcatgctcgc acgtcgcgcg
     6421 ccgcgggcaa gacggccgag caagcggccg cagacctcaa gctgccggcg aagttcagcg
     6481 gttacaactt gaccgcccgc ggcggtccgg cgaacaacct cggcatcatc tataccgagc
     6541 tcggtagtcg ttaaacgcca cgagtcgtat gtcgaacggt agccgctggc cggccgtcgc
     6601 cgtgctgtgt ctcgccgctg tgagcgcgtg caggcagtcg ccggccggca acgccgcgag
     6661 cccgagcacg gcgccctcgc cgcccgttat gaccgtcaat cagctgatgc acggcatcct
     6721 atttccggcc gccaataccg tgttttacgc ccaagccgac gacccggccg cggtaccgcg
     6781 cgacccgatg ccgtcgtcgt cgacgaattc gttatcggga ctgtacggcg gttggcaggc
     6841 cgtcgagaac agtgcgctcg tgctcgtcga gtccgccgac ctgatgctga ctccggggcg
     6901 catgtgcgag aacggcgagg tcgtcgacgt cggcaacgaa gactggatcc gcttcgtcgc
     6961 cgactaccga gagaagagtc tggccgcgta cgaagcggcg ctcacgaaga accaggacac
     7021 catggtcgac gcgagcggtt acctttcgga agcttgcctc gcgtgtcacc gcgtctatcg
     7081 acgcgagccg acgcccggca tcacgacgca gcgttgcata ccgggtgctc cgacgcgcta
     7141 gatgacctgc tgatcctcgc cgacgatgac ttcgccgtcg aactcggcgc gcacgccgtc
     7201 gatgaacgac gagatcgtga aaccgttggc ggcctggccg cgcacttggt gattcagcac
     7261 gatcgtcttg acgccggctt cgcgcgccat gcggcccgcg tccgcaggcg tcacgtgcgt
     7321 ctccgccacg tggcgaaaga cgttctcggg gcgcccggcc gcggcctctt ccttggcgcg
     7381 cgccatgttg gtttcgtagt tgcggtggtc catgacctcg cagacgaaga ggtcggcgcc
     7441 gcgcgcgagc ttcacgatgt tggccgagta cgccgtgtcg cccgagaagc aaatcgagcg
     7501 cgtgctcgtc tcgaagcgat aaccgagcga gcggtgctgc atcttggcaa cgaagcgctc
     7561 ggggaagtgc gtgttcgtcg ccgccgtcac gacgacgcgc tcgtcggcga acacgcgcac
     7621 cgggtcgttg ccgacgtcga gatcgtggcc aaagaacagc tcctcgggct tgctcgtgcg
     7681 cccctcgtcg accgtgcgga tctcgacgtt ggcgcggaaa aaagccaaag cgcctgccac
     7741 ggtgctctcg gtcgcgtagg ggccgtagac gctcgtcggc gccgtcttgg tgcccgtcca
     7801 ctggtgcgcg agcagcgcgg cgagatcggc cgtgtggtcg tcgtgcaagt gcgtgaggaa
     7861 aatcgtgttg acggcttggt agcccaagcc cgaggcgacg agcgcgcgca tcgtgccgta
     7921 gccgcaatcg acgagatacg ggacgccgtc gacgacgacg gcgctggccg tttcgccgcg
     7981 atcgagcgag acgccggggc cgccgcgtgt gccgagcatc acgagccgcg tgcccgtcgg
     8041 cgggaacgtc cggttcgcgg ccggcggagg ctgcgccgac agcggtagcc gccagccgag
     8101 cgcggcaagc ccggcggcgc ccgcgagcac ggtgcgtcgt gtagcggtcc ttgggtcgtc
     8161 gcggttcgtg ctcatcttaa aggggttttt cgcgcgaggg tggccaactc tagcctgccg
     8221 ttcggccgca gtcatcggcg gctcgcatga gcaccggcaa ttcggcacaa tcgctacccg
     8281 gcccgccacg atcgtgccgg ccaaaatcac cgatgaacga agacgtcatc cgcatccgcg
     8341 gggcgcggca gaacaatttg cgttctgtcg acctcgactt gccgctcggc gagctcatcg
     8401 tcgtgacggg cgtcagcggc tcgggcaagt cgtcgctggc gttcgacacc gtgtactccg
     8461 aagggcagcg gcgctacgtc gagacgttct cgccgtacgc acggcagttc ctcgatcgca
     8521 tggacaagcc gcaggtggac cgcatcgaag gcattccgcc cgcgatcgcg atcgaccaga
     8581 cgaatcccgt ccgcacgtcg cgctcgaccg tcggcacgat gacggagctc aacgatcacg
     8641 tgaagctctt gtacgcccgc gctgcgcggc tctattgccg caactgcggc cggcccgtgc
     8701 accgcgacga cgcggagtcg atctacgagg atctcgccca cgagccggcg ttgcgagccg
     8761 cgggcgcacg cgcgctcgtg acgttcacga tcaacgtccc cgagaatttc agcc
//
LOCUS       DATLEE010000434         2017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3237410, whole genome shotgun sequence.
ACCESSION   DATLEE010000434 DATLEE010000000
VERSION     DATLEE010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3237410"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="VM692_09295"
     CDS             <1..1546
                     /locus_tag="VM692_09295"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HUQ52405.1"
                     /translation="GHSHRAVKLHTAEQAKAAGILIYSDPADDGFKRGETWPRGYWRT
                     EDQIQRGNAKYSWFWHGDPLTPGVAAVADAQRLDPATAPTLPRIPAAVLSWGEARKIL
                     ERLDGPTAPPQFQGAMPITYRVGGDAVRVRLRVQMDDGLRTIRDVVARVPGAQQADRE
                     VILGTHHDAWTFGGVDPGTGTAALLEVARGLGELRRSGWQPKRTITLAFWDAEEFGLI
                     GSTEYAEHRLRELRDGAICYINTDLYMNGPLDAGGTPSLRDFVVEVTKDITDGTGSVY
                     DGWRGNKELKSLGSGADFVPFQDFVGLPTLSLEFNATGGYTYGAYHSNYDTRYFVEHV
                     ADPGFERGAQLVRLLGTTALRLGQSEVLPFRFSHYARRLAEFADTAAGWGQEEDGRPP
                     FAADLTSVKAAIARVAEAAASLERRLDEGLTAGRVSAAKAAPVNDALARLEQRLLDEN
                     QQWYRHVIYGWNIYSLYDGQPFPGLAEAIRLRDTDRTRLETARIVAAIDRLHAGLRAA
                     RGLADW"
     gene            1775..>2017
                     /locus_tag="VM692_09300"
     CDS             1775..>2017
                     /locus_tag="VM692_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HUQ52406.1"
                     /translation="MRRVTRSFALVVALALAASGCARQPPAAPTRFAGAPVVLISIDT
                     LRADHLAAYGYRAGSTPAIDRLAQAGVVFDDAYSQSP"
BASE COUNT          339 a          670 c          686 g          322 t
ORIGIN      
        1 cggacatagc catcgcgcgg tcaaactgca cactgcggag caggcgaagg cggccgggat
       61 tctcatctac agcgatcccg cggacgacgg gttcaagcgc ggcgagactt ggccgcgtgg
      121 ttattggcgg accgaggatc agatccaacg cggcaatgcg aagtacagct ggttctggca
      181 cggcgatccc ttgacgcccg gtgttgccgc cgttgcagat gcgcagcggc tcgaccctgc
      241 caccgcgccg acgctgccgc ggattccggc cgcggtcctc tcgtggggag aggcgcgcaa
      301 gattctcgaa cggctggacg gcccgacagc gccgccgcaa ttccaaggcg ccatgcccat
      361 cacctatcgc gttggcggcg acgcggtccg cgtcaggctg cgcgtgcaga tggacgatgg
      421 cctcaggacc attcgcgacg tcgtcgcgcg cgtgcccggc gcgcagcaag ccgatcgtga
      481 agtgatcctc gggacgcacc atgacgcgtg gacgttcggc ggcgttgatc cgggaaccgg
      541 tacggctgcg ctgctcgagg tggcgcgcgg gctcggcgag ctgcggcgca gcggctggca
      601 gcccaagcgg accatcacgc tcgccttttg ggacgcggag gagttcggtc taatcggctc
      661 gacggagtac gcggaacatc ggctgcgcga gcttcgggac ggcgccatct gctacatcaa
      721 caccgacttg tacatgaacg gaccgctcga cgccggcggc acgccgtcgt tgcgcgactt
      781 cgtcgtcgag gtgacgaagg acatcaccga cggaacagga tcggtgtacg acgggtggcg
      841 cggcaacaag gagctgaagt cgctcggcag cggggccgac ttcgtgccgt ttcaggattt
      901 cgtcgggctg ccgacgctct ctctcgagtt caacgcgacc ggcggctaca cctacggcgc
      961 ttaccattcg aattacgaca cgcgctactt cgtcgagcat gtcgccgatc ccggattcga
     1021 gcgcggcgca cagctcgtgc gtctgctcgg cacgacggcg ctgcgtctcg gccagtccga
     1081 ggtgctaccg tttcgtttct cccactacgc gcggcgcctg gcagagtttg ccgacacggc
     1141 tgcggggtgg gggcaggagg aagatggccg gccgccgttc gcggccgacc tgacgtcggt
     1201 gaaagccgcg atcgcgcgcg tcgccgaggc agccgcgtcg ctggaacgcc gactagacga
     1261 agggctgacc gccggccgag tgagcgcggc gaaggcagcg cccgtcaacg acgcgctcgc
     1321 gcgcctcgag cagcggctgc tcgacgaaaa ccagcagtgg taccggcacg tcatctacgg
     1381 ctggaacatc tactcgctct acgacggcca gccgtttccc ggcctcgcgg aggcaatccg
     1441 gctgcgcgat accgatcgca cgcggctcga gaccgcgagg atcgtggcgg cgatcgatcg
     1501 gctccacgcc gggctgcgcg cggcgcgtgg actcgccgac tggtgagaca ccaggtactt
     1561 tcgaacgcaa agctcgcaac gcccgcagag cacgacactc tccgatctga acgcaaagcc
     1621 cgcaacgcac gtactcttga acgcaaagct cgctaagcac aacactgcga tctgaacgca
     1681 aagcccgcga agcccgcaaa gcacttactc gtcccgactc tgcgtgttgt gcgagttctg
     1741 cgctgaacgt tcgatcgccg tcgacgcggg taaaatgcgg cgagtgaccc gttcgttcgc
     1801 attggtggtt gcgctggcgc ttgcagcgtc cggctgcgcg cggcagccgc cagcggcgcc
     1861 gacgcggttt gcgggcgcgc ccgtcgtcct catttcaatc gacacgctgc gtgccgatca
     1921 cctcgcggcg tacgggtatc gcgcgggctc gacgccggcg atcgatcggc tggcgcaggc
     1981 gggtgtcgtc ttcgacgatg cctatagcca gtcgccg
//
LOCUS       DATLEE010000435         5216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4958474, whole genome shotgun sequence.
ACCESSION   DATLEE010000435 DATLEE010000000
VERSION     DATLEE010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4958474"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1156
                     /locus_tag="VM692_09305"
     CDS             173..1156
                     /locus_tag="VM692_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HUQ52407.1"
                     /translation="MRDAIVVGSSAGGVRALRSLVGELPADLPAAVLVVNHIPAGSRS
                     ALPVILDEAGPLTAKPAEDGERLKHGTVYVAPPDRHLLVEKGRLRLTRGPRENRVRPC
                     IDTLFRSAAVDLGPRVIGVVLSGTLDDGTAGLWAIKDRGGVAIVQRPTEAEYPDMPEN
                     AMKHVDVDHVLPAAEISAKLAELVRERTGSAMPVPEAMAAEAALELHGSALKEGVLSL
                     GKASPNACPECHGSLIEINQGSITRYRCHTGHGFSLQTLMSETEVQIERSLWSAVRAI
                     EERSFLLRSMEEDARETGNPELGERLAAAADRDEENAQGVRSLIVGSSSAR"
     gene            1269..3770
                     /locus_tag="VM692_09310"
     CDS             1269..3770
                     /locus_tag="VM692_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HUQ52408.1"
                     /translation="MSSNANTPPRVPVVGIGSSAGGIDALRQFFTAVSPELGVAYVVI
                     VHLSPDHESELASILGRCTTMSVVEVGDSKALQLTPNCVYVIAPDRKLEITADTIAAA
                     PFADLRERRAAIDVFFRSLAHTHGDGFAIVLSGGGSDGSVGARAVKEAGGVVIVQNPQ
                     EAAHDAMPRAVIAAAVADLVLPVNDIALRLAELVHNRRIIAPLVQQQPRGESGPGDEA
                     ALHRIFDLLRARTGHDFTRYKRPTILRRLARRMQLGHRATFDHYLTLLREQPQEVQAL
                     FDDLLISVTAFFRDPATWEALRVQVVAPLVERSQGEESLRVWVPGCATGEEVYTLAIL
                     FREEMARRDLATELVIFGSDVDQGGLATAREGSYPLAIAADISEARLARYFRAEGEHY
                     RVTNEIRDSVVFAVHSVLRDPPFSKLNLISCRNLLIYLDRELQQQLQNVFRYGLRDDG
                     YLLIGVSETADPELFDPVDKQNRIFRARMRAAGPFVRLPHLSDVPHMQPAVDRARERS
                     VRARRSSAEMHIEALEEVAPPSILIDEHWNVEHLSEAAGRFLQPRGGPPARSLIDLVR
                     PELVEELRAALHRAVELHEPTLSTFVPVRFNGHAKLVGSLVQPRMRVEGHVQQLLVTF
                     LDAGDAGALDPIERGESTDAMVTALRDKLRIAEQRLETMRQEHSLAYEDLRAANEELQ
                     SLNEEYRSTTEELETSKEELQSVNEELQTVNNELKMKLEEVSRANNDLENFMSATDIP
                     MLFLDRGLCIKRYTAPLKHIFNVKRHDQGRPIGDLTHNMVYGDLEQDAARVLVDLAPI
                     ERQVGSRDGGTLLVRIRPYRTSEDKIDGVIVTFVR"
     gene            3841..>5216
                     /locus_tag="VM692_09315"
     CDS             3841..>5216
                     /locus_tag="VM692_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HUQ52409.1"
                     /translation="MAREIEREQVPAEPPAEAAARAEPFVVAIGASAGGLEALRTLFG
                     NMPGDSGATFVVVAHRSPHQDSRLVELLQPYTELPVRAVERTTPLEPDTVFILQPSAG
                     VTAVDTHARLTTAAARSGDRARIDQLFRAVAAGHGGRSIGVLLTGAGSDGAIGLRQIK
                     DAGGLVIVQEPEEAEYDGMPRSAIEAGAADLVLPLREIAQAIPRYCNSPLRLSPTELR
                     DVGAAGEPAPLVELCALLSARVNRDFSVYESSFVADRVAKRMRLRGLDRFPEYLRLIA
                     AEADESIALASDLLQNATQFFGDAERARRLEQDLLPQIFDRKSTEHDAVRVWCVGCGT
                     GENAYSVAMQLLEESMRRRVQPHVQVFASDPVEDLLQRARLGLYPPEIADSVSAVRLA
                     NFFMRDERGYRVRPLLRRAVTFACHDVFSDFPFAHLDLIVCRRRLLHDLKPGVRRSAL
                     HSFHFALEP"
BASE COUNT          857 a         1748 c         1727 g          884 t
ORIGIN      
        1 tgcggctgca gctctcctac cgcctcgccg ctccgacgcg gtagtcgcca gccgcgggcc
       61 gacgagccga acatcgcggc ctctgcctgc tttggcacag gccttgcacc tatcggcccc
      121 tacgcgtcgc gcttgcattc cgccggcgca gggcgtcgaa gcgggtgccg ccatgagaga
      181 tgccatcgtc gtgggttctt cggcgggcgg cgttcgcgcg ctgcgctcgc tcgtcggcga
      241 gctgccggcc gatctgccgg ccgcggtgct cgtggtcaac catattccgg cagggtcgcg
      301 aagcgcctta ccggtcattc tcgacgaagc gggtccgttg accgcgaaac cggccgagga
      361 cggcgagcgc ctgaaacacg gcacggtcta cgtggccccg ccggaccgac atctgttggt
      421 cgaaaagggc cggctgcggc tgacgcgggg gccgcgcgaa aaccgtgtcc ggccctgcat
      481 cgacacgctg ttccggtccg cggccgtcga cctgggcccc cgcgtcatcg gcgtcgtgct
      541 gtcgggcacg ctcgatgacg gcactgcagg actttgggcc atcaaggacc gcggcggcgt
      601 cgcgatcgtc caacggccca ccgaggccga ataccccgac atgccggaaa acgccatgaa
      661 gcatgtcgac gtcgaccatg tcctgcccgc ggccgagatt tcggcgaagc tcgcggagct
      721 cgtgcgcgag cgtaccggtt cggcgatgcc ggttcccgag gcgatggccg cggaggccgc
      781 gctcgagctg cacggcagcg cgctgaagga aggtgtattg agcttgggga aagcctcgcc
      841 gaacgcctgc cccgagtgcc acggctcact gatcgagatc aaccagggca gcatcacgcg
      901 ctatcgctgc catacgggcc acggcttctc gctgcagacg ctgatgtcgg agacggaagt
      961 ccagatcgag cggtcgctct ggagtgcagt gcgcgccatc gaggaacgat cgttcctgct
     1021 gcgctcgatg gaagaggatg cgcgcgagac cggcaacccc gagctcggcg agcggctcgc
     1081 cgccgcagcg gaccgcgacg aggagaacgc gcaaggcgtg cgctcgttga tcgttggatc
     1141 gtcatccgcc cggtaagcgc gcgtacgaaa tgccttgccg ctctgaggag agcggctcag
     1201 aatcgaaccg ccagggactg tttaggacga gtccctctcc ggctagaacc tccaacaatc
     1261 gagcgctaat gagcagtaac gcgaatactc cgccacgcgt gcccgtggtg ggcataggat
     1321 cgtcggcggg cggtatcgac gcattgcgcc aattcttcac ggccgtgtcc cccgagctcg
     1381 gcgtggccta tgtcgtcatc gtccacctct cgcccgatca cgagagcgag ctcgcgtcga
     1441 tcctgggccg ctgcaccacg atgtcggtcg tcgaggtcgg cgacagcaaa gcgctgcagc
     1501 tgacgccgaa ctgcgtctac gtcatcgcgc ccgaccgcaa gctcgagata acggcagata
     1561 cgatcgcagc ggcaccgttc gccgacctgc gtgaacgccg cgccgcgatc gacgtgttct
     1621 tcaggtcgct cgcccacact cacggcgacg gctttgcgat cgtgctgtcg ggcggcggct
     1681 ccgacggctc ggtcggcgcg cgagccgtca aagaagccgg cggcgtcgtg atcgtgcaga
     1741 acccgcagga ggccgcacac gacgcgatgc cgcgcgccgt cattgcggcc gccgttgccg
     1801 acctcgtttt gccggtcaac gacatcgcgc tgcggctcgc ggagctcgtg cacaatcggc
     1861 gcatcattgc gccgctggtc cagcagcagc cacgcggcga gagcgggccc ggcgacgaag
     1921 ccgcgttgca ccgtatcttc gatttgcttc gcgcgcgcac aggccacgac ttcacgcgct
     1981 acaagcggcc gacgatcttg cggcgcctcg cgcgacggat gcagctcggg caccgtgcga
     2041 cgttcgatca ctatctaacg ttgcttcgcg agcagccgca ggaagtccag gcgctgttcg
     2101 acgacctgct gatctccgtc acggcctttt tccgtgaccc tgcgacgtgg gaagcgctgc
     2161 gtgtccaggt cgttgcgccg ctagtcgagc gctcgcaagg cgaagaatcg ctgcgcgtct
     2221 gggtgccggg ctgtgcgacc ggcgaggagg tctacacgct ggcgatcctg tttcgcgagg
     2281 agatggcgcg ccgcgatctg gccaccgagc tcgtcatctt cggctccgac gtcgaccaag
     2341 gcgggctcgc gacggcgcgt gaaggcagct atccgctcgc gatcgcggca gacatctccg
     2401 aggcgcggct cgcgcgctac ttccgcgccg agggcgaaca ctatcgagtg acgaacgaga
     2461 ttcgcgattc ggtcgtcttt gccgttcaca gcgtcctgcg cgacccgccg ttctcgaaac
     2521 tgaacctgat ttcgtgtcgc aatttgttga tctacctcga ccgcgagctg cagcaacaac
     2581 tgcagaacgt gttccgttat ggcttgcgtg acgacggcta tctgttgatc ggcgtatccg
     2641 agacggcgga tcccgagttg ttcgaccccg tcgacaaaca gaaccgcatt ttccgcgcgc
     2701 gcatgcgggc ggccggacct ttcgtgcgct tgccgcacct atcggacgtc ccgcacatgc
     2761 agccggctgt cgaccgtgcc cgcgagcgct cggtgcgcgc tcgccgctcg tctgccgaaa
     2821 tgcacatcga agctctcgag gaagtggcgc ccccgagcat cctgatcgac gagcactgga
     2881 acgtcgagca tttgtcggag gccgccggtc gattcttgca gccgcgcggc ggtccgccgg
     2941 cccgctcgct gatcgacctc gtgcggccgg agctcgtcga agagctgcgc gcggcgctgc
     3001 accgcgccgt cgagctgcac gagccgactc tgtcgacgtt cgtgcccgtg cgcttcaacg
     3061 gccacgcgaa gctcgtgggc tcgctcgtgc agccgcgcat gcgcgtcgag ggccacgtgc
     3121 agcagctgct ggtcacgttt ctcgacgcgg gcgatgccgg tgcgttggac cctatcgagc
     3181 gcggcgagtc gaccgatgca atggtgacgg cgctgcgcga caagctgcgc atcgccgagc
     3241 agcgcctcga gacgatgcgc caggagcaca gcctcgctta cgaggacctg cgcgccgcga
     3301 acgaggagct gcaaagcctc aacgaggaat atcgctcgac cacggaagag ctcgagacca
     3361 gcaaggaaga gctacagagc gtcaacgagg agctgcagac cgtcaacaac gagctgaaga
     3421 tgaagctcga ggaagtgtcg cgagccaaca acgacctcga gaacttcatg tcggcgaccg
     3481 acattccgat gttgtttctg gatcgcggtc tgtgcatcaa gcgctacacg gcgccgctca
     3541 agcacatctt caacgtcaag aggcatgacc agggccgacc gatcggcgac cttacgcaca
     3601 acatggttta cggcgatctc gaacaggatg cggcccgcgt gctcgtcgac cttgcgccca
     3661 tcgagcgcca agtcggttcg cgcgacggcg gcacgctgct cgtgcgtatt cgcccgtacc
     3721 gcacatcaga ggataaaatc gacggcgtga tcgtcacctt cgtcagatga cctgacgatt
     3781 ttcgacgcgg ctatcagggg cgagtggcag gaaccccact gtcgagtaga gggatttgca
     3841 atggcgcgcg agattgagcg agagcaggtg cctgcggaac cgcccgccga ggcagctgcg
     3901 cgcgccgagc cgttcgtcgt cgcgattgga gcctcggccg gcggcctcga ggcgctgcgc
     3961 acgttgttcg gcaatatgcc gggagactcg ggagcgacgt tcgtcgtcgt ggcccatcgc
     4021 tcgcctcacc aggacagccg cctcgtcgag ttgctccagc cctatacgga gctgccggtt
     4081 cgcgccgtgg aaaggacgac gccgctcgag cccgacaccg tattcatcct gcaaccgtcg
     4141 gccggcgtca ccgccgtcga cactcacgcg cggctcacca ccgccgcggc tcgcagcggc
     4201 gaccgtgcgc gtatcgacca actgttccgg gccgtggccg cggggcacgg cggccgcagc
     4261 atcggcgtgc tactgacggg cgcgggctcg gacggtgcga tcgggttacg acaaatcaag
     4321 gatgccggcg gcctggtcat cgttcaggag cccgaggagg ccgagtacga cggcatgccg
     4381 cgcagcgcga tagaggcggg cgccgccgac ctcgtgctgc cgttacgcga gattgcgcag
     4441 gccatcccgc ggtactgcaa ttcaccgcta cggctgtcgc cgaccgagct gcgcgacgtc
     4501 ggcgctgccg gcgagccggc gccgttggtc gagctctgcg ctttgttgag tgctcgagtc
     4561 aaccgtgatt tttcggttta cgaatcgtcg ttcgtcgcgg accgcgttgc caaacgcatg
     4621 cgcttgcgcg gtctcgaccg cttcccggag tatctgcggt tgatcgccgc cgaggccgac
     4681 gagagtattg cgctcgcgag cgatctgctg cagaacgcga cgcagttctt cggggatgcg
     4741 gagcgtgcgc ggcggctgga gcaagacctg ctgccgcaga ttttcgatcg caagtcgacc
     4801 gagcacgacg ccgtgcgcgt ctggtgcgtc ggctgcggga ccggcgagaa cgcctattcg
     4861 gtcgcaatgc agctgctcga ggaatccatg cggcggcggg ttcagccgca cgtgcaggtc
     4921 ttcgccagcg accccgtcga ggacctgttg caacgcgcgc ggctgggcct ctatccaccc
     4981 gaaatcgccg actccgtttc ggccgtcagg ctcgccaatt tcttcatgcg cgatgagcgc
     5041 ggctaccgcg tgcggcccct gctgcgcagg gccgtgacct tcgcctgcca cgacgtattc
     5101 agcgactttc cattcgcgca tctcgacctc atcgtgtgtc gccggcggct attgcacgat
     5161 ctgaagcccg gcgtgcggcg ctcggcgttg cacagcttcc acttcgcgct ggagcc
//
LOCUS       DATLEE010000436         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2724961, whole genome shotgun sequence.
ACCESSION   DATLEE010000436 DATLEE010000000
VERSION     DATLEE010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724961"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VM692_09320"
     CDS             complement(<1..289)
                     /locus_tag="VM692_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HUQ52410.1"
                     /translation="MDPISTTIFSGKAHPALARDIAFHLGLSLGRITVSRFSDGEISV
                     EIMENVRGRDVFIVQPTCPPVNENLMELLIMADACQRASAARVTAVIPYFGY"
     gene            complement(325..399)
                     /locus_tag="VM692_09325"
     tRNA            complement(325..399)
                     /locus_tag="VM692_09325"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(365..367),aa:Gln,seq:ttg)
     gene            complement(432..1046)
                     /gene="lolB"
                     /locus_tag="VM692_09330"
     CDS             complement(432..1046)
                     /gene="lolB"
                     /locus_tag="VM692_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HUQ52411.1"
                     /translation="MTARAVPALLAAAMLGGCASLPTGTDGLSLAQRRDMLESVQAWD
                     MRGRLVVDTGERAVQGRFNWHQDSDTLELTVRNPLGAGILQVEGRPEALTVTARGETR
                     TLTDPEAELSELVGWWLPITSLPHWLLGFPDSMFRATTEAGADGTLAGLEQRLWRVAY
                     PTYGLAPIAATGEQVLVPSHIDLTHGDLTLKLTIDDWQADTEAP"
     gene            complement(1043..>2004)
                     /locus_tag="VM692_09335"
     CDS             complement(1043..>2004)
                     /locus_tag="VM692_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ52412.1"
                     /translation="AFLAMTEQRIDDAKGYFNELRGTPNYRNESFYYLGRLAETERDF
                     LQATRSYARVTEGTHAVEAQLRSARIMYGEQSDRDGAIRHLRDFGEANPRFAADMLVA
                     QAQLFVQMQQPEEAMRVLDEALKASPDDPALQAAHAQLYVFRAQDIIERGQLDEAEAL
                     LNEGLARHPDNSALRYSMALLYEDQGRNRKALDVLAALAKENPDDAAILNAYGYLLTD
                     HFDKHDQARDYIERALALNPDSPAIIDSMGWVLFKLGDYRGAADYLERAYRLEQDPEI
                     AAHLVDARWQLGERDSARELLRQSLEANPDSDHLKEVDERLEP"
BASE COUNT          310 a          646 c          683 g          365 t
ORIGIN      
        1 cataaccgaa gtacggaatc acggccgtca cgcgtgcggc ggaagcacgc tggcacgcgt
       61 cggccatgat caagagctcc atgagatttt cgttcacggg cgggcaagtc ggttgcacga
      121 tgaagacgtc gcgcccgcgc acgttctcca tgatctctac gctgatctcc ccgtcgctga
      181 agcggctcac cgtgatgcgg ccgagcgaga ggcccaagtg gaacgcgatg tcgcgcgcca
      241 aggcagggtg agccttgccg gaaaaaatcg tcgtcgagat ggggtccaac ggcgcggctc
      301 ctggtagtta ttgttctatt cgattggctg gggcggtagg gatcgaacct acgtatgacg
      361 ggatcaaaac ccgttgcctt accgcttggc tacgccccag cctatattgt gctgggcgcg
      421 cgccgtttca ttcaaggcgc ttcagtgtcc gcctgccaat cgtcgatcgt gagcttcagc
      481 gtcagatcgc catgcgtgag atcgatgtgg ctcggcacga gcacttgctc gccggtcgcc
      541 gcgatcggcg caagtccgta cgtcggatac gcgacgcgcc agagtcgctg ctcgaggccg
      601 gcgagcgtgc cgtccgcgcc ggcctcggtc gtcgcacgaa acatgctatc gggaaacccg
      661 agcagccaat gcggcaggct cgtgatcggc agccaccagc cgacgagctc cgaaagctcc
      721 gcctcagggt ccgtcaacgt gcgagtctcg ccgcgcgccg tgacagtcag cgcctcgggt
      781 cggccttcaa cttgcaaaat tccggcgccg agcgggttgc gcaccgtgag ctcgagcgtg
      841 tcgctgtctt ggtgccagtt gaatcggccc tgcacggcgc gctcgcccgt atcgacgacg
      901 aggcggccgc gcatgtccca ggcctgcacg gactcgagca tgtcgcggcg ctgggcgagg
      961 ctcaatccgt ccgtgcccgt cggtagcgag gcgcagccgc cgagcatcgc cgcagcgagc
     1021 agcgctggga cggcgcgcgc cgtcatggct cgaggcgctc gtcgacctct ttcagatgat
     1081 cgctgtcggg gttcgcttcg agcgactggc gcagcagctc gcgggcgctg tcgcgctcgc
     1141 cgagttgcca acgcgcgtcg acgaggtgcg ccgctatttc gggatcctgc tccaggcgat
     1201 acgcgcgctc gaggtaatcg gccgcaccgc ggtagtcgcc gagcttgaac aagacccagc
     1261 ccatgctgtc gatgatcgcc gggctgtcgg gattcagcgc gagcgcgcgc tcgatgtaat
     1321 cgcgcgcttg atcgtgcttg tcgaagtggt ccgtcaaaag gtagccgtag gcgttcaaga
     1381 tcgcggcgtc gtcggggttc tccttggcga gcgccgcgag cacgtcgagc gccttgcggt
     1441 tgcggccctg gtcctcgtag agcagcgcca tcgagtagcg cagcgcgctg ttgtcgggat
     1501 gacgcgcgag cccttcgttg agcagcgctt cggcctcgtc gagctggccg cgctcgatga
     1561 tgtcttgcgc gcggaacacg tagagctgcg cgtgcgcggc ttgcagcgcc gggtcgtccg
     1621 gcgacgcctt cagcgcctcg tcgaggacgc gcatcgcttc ctcaggctgc tgcatctgca
     1681 cgaacagctg cgcctgcgcg acgagcatgt cggccgcgaa gcgcgggttc gcttcgccga
     1741 agtcgcgcag gtggcggatc gcgccgtcgc gatcgctctg ctcgccgtac atgatgcgag
     1801 ccgagcgcag ctgcgcctcg acggcatgcg tgccttccgt gacgcgcgcg tacgagcgcg
     1861 tggcttgcag gaagtcgcgc tcggtctcgg cgagcctgcc caagtagtag aaggactcgt
     1921 tgcgatagtt cggcgtgccg cgcagctcgt tgaagtagcc cttcgcgtcg tcgatgcgtt
     1981 gctccgtcat cgcgaggaac gcga
//
LOCUS       DATLEE010000437         5704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1978158, whole genome shotgun sequence.
ACCESSION   DATLEE010000437 DATLEE010000000
VERSION     DATLEE010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978158"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VM692_09340"
     CDS             <1..398
                     /locus_tag="VM692_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HUQ52413.1"
                     /translation="DLVLESRPVIAAVLAAQSLDPAKALPMLRGIQHAHYERGQHVVR
                     EETLRAIAAEIGLDAAAFDAARKSVPVDEHIAASQELMNNVGAQGFPTFVLQIGNDWF
                     AVPHGRFASAPAKFAEWLDSQLRANSRTH"
     gene            419..1405
                     /locus_tag="VM692_09345"
     CDS             419..1405
                     /locus_tag="VM692_09345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUQ52414.1"
                     /translation="MVPLSILDLAFVPEGATPADALHRSLELARHAEQCGYRRFWLAE
                     HHNMVGVASAATSLVIGYIAAGTRTIRVGAGGIMLPNHSPLVIAEQFGTLESLYPGRI
                     DLGLGRAPGTDMLTLRALRRNHTSADSFPDDVLELQALFGALQPNQAVQAVPGTELEV
                     PLWILGSSLYGAQLAAALGLPYAFASHFAPAELDAALALYRAQFRPSAQLAAPYAMVG
                     INAVVADTDADARRLFTTAQQGFTNLLRGARGRMQPPIDDIEAYWTPAEKARASHMLG
                     CSFVGSPSTVREQLSDFVERTRADELIVAAAIYDQAARLRSYELLAETVAPR"
     gene            complement(1402..1839)
                     /locus_tag="VM692_09350"
     CDS             complement(1402..1839)
                     /locus_tag="VM692_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52415.1"
                     /translation="MSEGQLKRVVQPDGTVRMTAEAAAFQFARPKPDVLVIKITGYDK
                     GQFATAPFDEIGSILRVATPLELFIDARDAVGATVRVSQDWTSFLALHKADLRHVHVL
                     AGSKMVELTVAITRHLSRTGNLLQIYSDPEIFAAQLAAASRRR"
     gene            1944..3110
                     /locus_tag="VM692_09355"
     CDS             1944..3110
                     /locus_tag="VM692_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ52416.1"
                     /translation="MQRLFAALLACVASTAAAQDLVITNAHIVVGNGAVIERGSIVIR
                     GGRIASVAAGAAAPAGLTPIDLRGMTAMPGFIDGHRHVNTGPDEKVEMQALLDAGYTT
                     VLSGGGPAEGNITLRDHIESGQIRGPRIIPSGRLDLAESSTDQVRAETRRLAALGVKW
                     IGEMALTPKPNPTPKELENLRALLDEARKVGIIVQIHAVSPQSMMAAVDAGVKKLVHT
                     PHFGWLTFAEAQRVAAAEVQELSTIGFGVPVFGVFANDNQPRFRDGKPWPESILQGDG
                     RGQEAGYKAVNARTIWDAGVVYGYGTDTNYDPKAGVAHELRALNLMFSMQDVVKLMGP
                     NSAAYIEMSKELGTLEAGKLADIVVLDGNPLEGYWNYLRTKLVLKGGVIVSDQR"
     gene            3181..4557
                     /locus_tag="VM692_09360"
     CDS             3181..4557
                     /locus_tag="VM692_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ52417.1"
                     /translation="MKIEFVNHSSFIVQSGEVSLLCDPWIEGTAFNDGWALISPTRFG
                     YADFERVTHIWFSHEHPDHFAPPNLRKIPESLRRRIRVLYQGTLDRKVVDHCRKLGFG
                     DVQELALDRWLALAPDFEVLCGAYPRGDSWLAVRAGGKVLLNLNDCNIYTPEAARPVR
                     DLVGPVDALATQFSLSAWEGNPEEVERRRAGARTTLDRCVFHAQFFGAKQVIPFASFV
                     WFCHDENFYINAEHNRVDRAFEELRARSPCQPCVMYPGDCLTVGESYNSRLALERHIA
                     DFDALAARPRVQAKRVSEEELLDAARKFCAVLAQQAGRRRLEVGLARAQARRLLRRLS
                     RRVSLGDLGWLARNVLTLRPRAARIYLWDRGQAYLFDLRRGLRPSSLPQDQCEICCSA
                     DSLVYAFRFLWGGLTLQVNGRFHEVYADARASIFDYFHLTDSFNRGERFAQNSAVRRL
                     ARTLRLAS"
     gene            4572..5327
                     /locus_tag="VM692_09365"
     CDS             4572..5327
                     /locus_tag="VM692_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HUQ52418.1"
                     /translation="MDNDFRSRLKRREQLLGTMVTLASAASAEVLASLGFDWLFIDGE
                     HGPLGIRELNEILQAVSHKTACIVRVPEAAEVPIKRALDLGAHGIIVPQVNTAEQAAD
                     VVRWSRYAPEGARGVGLARAQGYGQNFREYLGAANREIAVIVQAEHARAVENIEAIAR
                     VPGVDAVLLGPYDLSASLGRMGEIDHPDVVEAIGRVTDVCNTAGMPLGFFGVTAAAVQ
                     PYAARGYTLLVAGVDTIYLANGAKALLKELRGA"
     gene            complement(5447..>5704)
                     /locus_tag="VM692_09370"
     CDS             complement(5447..>5704)
                     /locus_tag="VM692_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52419.1"
                     /translation="SLILAAGLGLDYALFFEHAADDAAEQARTLHAVLACAASTFMVF
                     ALLATTDLPVLRAIGAPVALGVVTNFVLALLLTRAPRAERT"
BASE COUNT          918 a         1883 c         1933 g          970 t
ORIGIN      
        1 cggacctcgt actcgagtcg cggcccgtga tcgcggccgt gctcgcggcg cagtcgctcg
       61 atcctgctaa agcattgccg atgttgcgcg gcattcagca cgcgcactac gagcgcggcc
      121 aacacgtcgt gcgcgaagag acgctgcgcg cgatcgccgc ggagatcggg ctcgacgccg
      181 ccgcgttcga cgcagcgcgc aaatcggtgc ccgtcgacga gcacatcgcg gcgtcacagg
      241 agctcatgaa taacgtcggc gcgcagggct tcccgacctt cgtgctgcag atcggcaacg
      301 attggttcgc cgtgccgcac ggccggttcg cttccgcgcc ggcgaagttc gcggagtggc
      361 tcgactcgca gctgcgcgcg aattcgcgca cgcactaggc gccgcgtcgg cgttagcaat
      421 ggtcccgctg tcgatcctcg atctcgcatt cgtcccggaa ggagcgacgc cggccgatgc
      481 gctgcaccgc agtctcgagc tcgcgcggca cgccgagcag tgcggttacc ggcggttctg
      541 gctcgccgaa caccacaaca tggtcggagt cgccagcgcg gcgacgtcgc tcgtgatcgg
      601 ctacatcgcg gcggggacgc gcacgatccg cgtcggcgcc ggcggcatca tgctgccgaa
      661 tcattcgccg ctcgtcatcg ccgagcagtt cggcacgctc gagtcgttgt acccgggccg
      721 tatcgatctt ggcctcggcc gcgcgccggg caccgacatg ctgacgttgc gtgcactgcg
      781 ccgcaatcac acgagcgccg actccttccc cgacgacgtg ctcgagctgc aagcgctgtt
      841 cggcgcgttg caaccgaatc aggccgtgca ggccgtgccg ggcacggagc tcgaggtgcc
      901 gctctggatt ctcggctcga gtctgtacgg cgcgcagctc gctgcggcgc tcggtttgcc
      961 gtatgcgttc gcctcgcact tcgcgccagc cgagctcgat gcggcgcttg ccctgtatcg
     1021 cgcgcagttc cggccgtcgg cgcagctcgc cgcgccgtac gcgatggtcg gcatcaacgc
     1081 cgtcgtcgcc gatacggatg ccgacgcgcg ccggctgttt acgacggcgc agcaggggtt
     1141 cacgaaccta ctgcgcggcg cacgcgggcg gatgcagccg ccgatcgacg acatcgaggc
     1201 ctactggaca ccggccgaga aagcgcgcgc atcgcacatg ctgggttgct cgttcgtcgg
     1261 gtcgccgtcg acggtccgcg agcaactgtc ggatttcgtc gaacgtacgc gtgccgacga
     1321 gctgatcgtc gcggcggcca tctacgatca ggccgcgcgg ttgcggtcct acgagctgct
     1381 cgctgagacc gtcgcgcctc gttaacgtct acgcgacgcg gccgccagct gagccgcgaa
     1441 gatctcggga tccgagtaga tctgcagcaa gttcccggtc cgcgacagat ggcgcgtgat
     1501 ggccaccgtc aattcgacca tcttcgagcc ggccagcacg tgcacgtggc gcaaatccgc
     1561 cttgtgaagc gcgaggaagc tcgtccaatc ctgactgacg cggaccgtgg cgccgacggc
     1621 atcgcgcgcg tcgatgaaca gctcgagcgg tgtcgccacg cgcaggatcg agccgatttc
     1681 gtcgaacggc gcggtcgcga actggccttt atcgtagccc gtgatcttga tcaccagcac
     1741 gtcgggcttc gggcgcgcga actggaaggc cgcggcctcg gccgtcatgc gaacagtgcc
     1801 gtccggttgc actacgcgct tgagttgtcc ttcgctcatg gctcgcgaag tatagcggcc
     1861 gccggcgaag caaccgccgc aaatggacct ggcagggttt ctcgtctaag ttctgttcgt
     1921 ctcgcgaacc gttaggggga aacatgcaac ggcttttcgc tgcactgctc gcgtgcgtcg
     1981 cttcgacggc ggcagcgcag gatctcgtca tcacgaacgc gcacatcgtc gtcggcaacg
     2041 gcgccgtgat cgagcgcggc tcgatcgtga ttcgcggcgg ccgcatcgcg tcggtcgcag
     2101 cgggcgcggc agcccccgca gggctcacgc cgatcgacct gcgcggcatg acggcgatgc
     2161 cggggttcat cgacggccac cggcatgtaa acaccggccc cgatgagaag gtcgagatgc
     2221 aggcgttgct cgacgcgggc tacacgacgg tgctttcggg cggcggccct gccgagggca
     2281 acatcacgct gcgcgaccac atcgagtcgg ggcaaattcg cggcccgcgc atcattccct
     2341 cgggccgcct cgatctggcc gagagcagca cggaccaggt gcgcgccgag acgcggcgcc
     2401 tggcggcgct cggcgtgaag tggatcggcg agatggcgct gacgccgaag cctaacccca
     2461 cgccgaagga gctcgagaac ctacgcgcgc tgctcgacga ggcgcgcaag gtcgggatca
     2521 tcgtgcagat ccacgccgtc agcccgcagt cgatgatggc agccgtcgac gcgggcgtga
     2581 agaagctcgt gcacacgccg catttcggct ggctcacgtt cgcggaggcg cagcgtgtcg
     2641 ccgcggccga agtgcaggag ctgtcgacga tcggcttcgg cgtgccggtg ttcggcgtgt
     2701 tcgcgaacga caaccagccg cgctttcgcg atggcaagcc gtggcccgag tcgatcctgc
     2761 aaggcgatgg ccgcggccag gaggcgggct acaaggccgt caacgcgcgc acgatctggg
     2821 atgcgggcgt cgtgtatggc tacggcaccg acacgaacta cgatccgaag gccggggtcg
     2881 cgcacgagct gcgcgctttg aatctcatgt tctcgatgca agacgtcgtc aagctcatgg
     2941 gcccgaactc cgcggcgtac atagagatga gcaaggagtt gggcacgctc gaggcgggca
     3001 agctcgcgga catcgtcgtg ctcgacggca accccctcga aggttactgg aattatttgc
     3061 gcacgaagct cgtgctgaag ggcggcgtga tcgtttcgga tcaacgctga gcgtcgtgaa
     3121 attcgtcgtc gatcgtcgcc gccctcttaa gatgcatccg aggccgcgtt gtaaccgcga
     3181 atgaagatcg agttcgtcaa ccactcgtcg ttcatcgtcc aaagcggcga ggtgagcctg
     3241 ctctgcgatc cttggatcga aggaacggcg ttcaacgacg gctgggcgct catctcgccg
     3301 acgcggtttg gctacgccga tttcgaacga gtgacccaca tctggttctc gcacgagcac
     3361 ccggaccact tcgcgccacc gaacctgcgc aagattcccg agtcgttgcg tcggcggatt
     3421 cgtgttttgt atcagggaac gctcgaccgc aaggtcgtcg accactgccg caagctcggt
     3481 ttcggcgacg tgcaggaatt agccctcgac cgttggctgg ccctcgcccc ggatttcgag
     3541 gtgctgtgcg gcgcctatcc gcgcggcgat tcctggctgg ccgtgcgcgc cggcggcaag
     3601 gtgttgctga acctcaacga ctgcaatatc tacacgccgg aagcggctcg ccccgtgcgc
     3661 gacttggtgg gacccgtgga cgcgctggca acgcagttct cgctctccgc atgggaaggc
     3721 aatccggagg aagtcgagcg ccgtcgcgcc ggcgcgcgca cgacgctcga ccgctgtgtg
     3781 ttccacgcgc agtttttcgg cgccaagcaa gtgatcccgt tcgcgagctt cgtttggttc
     3841 tgtcacgacg agaacttcta catcaacgcc gagcacaacc gagtcgaccg cgccttcgag
     3901 gagttgcggg cccgctcgcc ctgtcagccg tgcgtgatgt accccggcga ttgcctcaca
     3961 gtgggcgaga gctacaactc gcgactcgcg ctcgagcggc acatcgcgga tttcgatgct
     4021 ctcgccgcgc gcccgcgtgt gcaagccaaa cgcgtcagcg aggaggagct gctcgacgcc
     4081 gcccgcaagt tctgcgccgt gcttgcgcag caagccggtc gcaggcggct cgaggtcggc
     4141 ctcgcacgag cgcaggcgcg gcgtctgctg cgacgcctca gtcgtcgcgt cagtctcggc
     4201 gatttgggat ggctcgctcg taacgtgctg acgctgcgcc cgcgcgcggc gcgaatctac
     4261 ttgtgggatc gcggccaggc ttatctgttc gatttgcgcc gcggtctgcg gccgagctcg
     4321 ttaccgcaag accagtgcga gatctgctgt agtgcggatt cgctggtcta tgcgttccgg
     4381 ttcctatggg gcggcctgac gcttcaggtc aacggccgct tccacgaggt ctacgcggat
     4441 gcgcgcgcgt cgatcttcga ctacttccac ctaactgatt cgttcaaccg cggcgagcgt
     4501 tttgcccaaa actcggcggt tcgccgtttg gcgcgcacgc ttcggttggc gagctgacgc
     4561 cgaggctcat catggacaac gactttcggt cgcggctgaa gcgccgcgag caattgctcg
     4621 ggacgatggt cacgctcgcg agcgcagctt cggccgaggt gctcgcgagc ttgggcttcg
     4681 attggctgtt catcgacggc gagcacggcc cgcttggaat ccgcgagctc aacgagatct
     4741 tgcaagccgt gagccacaag acggcctgca tcgtgcgcgt gcccgaagcg gccgaggtgc
     4801 cgatcaagcg agcgctcgat ctcggcgcgc acggcatcat cgtgccgcag gtcaacacgg
     4861 ccgagcaggc cgccgacgtc gtgcgctggt cgcgctacgc gccggaaggc gcgcgcggcg
     4921 tcggcttggc gcgcgcccag ggctacggcc aaaacttccg cgagtacttg ggcgccgcaa
     4981 atcgcgagat tgccgtcatc gtgcaggcag agcacgcgcg cgccgtggaa aacatcgagg
     5041 cgatcgcgcg cgtgccgggc gtcgacgccg tgctgctcgg gccgtacgac ttgtcggcga
     5101 gtctcggcag gatgggcgag atcgatcatc ccgacgtcgt ggaggccatc ggccgcgtga
     5161 cggacgtatg caacacggcc ggcatgccgc tcggcttctt cggcgtcacg gcggcggccg
     5221 tgcagccgta cgcggcgcgc ggttacacgc tgctcgtcgc cggcgtcgac acgatctatc
     5281 tcgcgaacgg cgcgaaggcg ctgctgaagg agctgcgcgg ggcttaggcg ttcgccgtcg
     5341 acggcccgcg gccgaagtgt ccggagtcga tctccggcgt tcgttgcgcc agtgagtaac
     5401 cggcgaacgg ggcgaacgcg ccgcgtggcg cgtcgtagca atcactttag gtgcgttcag
     5461 ctctaggtgc gcgcgtcaac agcagcgcga gcacgaagtt cgtcacgacg ccgagggcga
     5521 cgggcgcgcc gatcgcgcgc aacacgggca gatccgtcgt cgcgagcagc gcgaacacca
     5581 tgaacgtcga cgcggcacaa gcgagcacgg cgtgcagcgt gcgggcttgc tccgcggcgt
     5641 cgtcggccgc gtgctcgaaa aacagtgcgt agtcgagccc caagccggcc gccaagatca
     5701 gcga
//
LOCUS       DATLEE010000438         6796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6955224, whole genome shotgun sequence.
ACCESSION   DATLEE010000438 DATLEE010000000
VERSION     DATLEE010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6955224"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VM692_09375"
     CDS             complement(<1..976)
                     /locus_tag="VM692_09375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUQ52420.1"
                     /translation="MDLQRLDSNSYGSPYAEQLKRGFRGLRFNGLLEKDFREFYIAQN
                     LPRARLSGLIALIVVLSVTCIDLLLGTNTAGTLNTLRLGILCPLLVVLGVAISVPSAQ
                     RYYTEVVAVGVTLIGFVVTYIAHLGALAGSSYVLAGLVLVVLYGCLFLGLLFNVAVTV
                     TGVMIAAHFVTGVAIGLPFDALYYSTAILGGAAVIGGISTYNLEYALRLNFLETRLLN
                     ELAERDGMTSLYNRRIFDDYMERLWRQSRRERTVIAIIFVDIDFFKVYNDLYGHQAGD
                     DCLKRVAKCISRGAKRPFDFAARYGGEEFVLVLYGPPDEYAQSVSEQIR"
     gene            complement(1064..2728)
                     /locus_tag="VM692_09380"
     CDS             complement(1064..2728)
                     /locus_tag="VM692_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HUQ52421.1"
                     /translation="MRGWAKAAATALCAWLALASALAFAQEPAIQATVDRAVVRINES
                     FTYVLRAEGAVRGEPEIAPLAAQFDILNTTSSRRIGVVNARASEVNEWQYQLMPKKAG
                     DFTIPPLRIGDRQSNSVPVRVLEPDPNASAAADIFMELSAEPDVIYVQSQVLFTLRLF
                     VGVSTGRATLTAPETTGVEAIVEKLGEDSQYQTSRGGRDFIVRERRYAVFPQQAGTLT
                     VGPVTFEAMVIPDRGFSRVQRFRSDVLELTVQPAVAPPPALAGAAWLPAQSVKLTEQW
                     SEPGDELAVGIPRTRTIGVEALGLLETQLPDVLLETQAGIRQYADRPELTREITADGL
                     LSRRTVGYAVIAQTPGDVTLAGVHLPWFNVATQRWEVAELAPLPLRITPSTDAEAAEP
                     AAAPAAAPSPVAARDRNPWPWISGVLALAWIATVALWWRNRGRAPRPVATKSPPAATP
                     KPLRKILRDLESACAVNDPEAARQALLKLGETRFTDNPPRSLGALAALLPNGAAREVL
                     ALEAHIYGAAAGAWRGEGLKAALGELENAGTAPAPPAGDPLLPLYR"
     gene            complement(2725..4557)
                     /locus_tag="VM692_09385"
     CDS             complement(2725..4557)
                     /locus_tag="VM692_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUQ52422.1"
                     /translation="MAEFHFLRPYWLLLAPVGAWLIWQLLRGRADGGGWRTVVDASLR
                     PHVLAEPEVLRDSRLALLAALAAWVLAVVGLAGPAWERLPVPAFRSDEALVVALDLSR
                     SMDAADVEPSRLARAKLKLLDLLERRAAGQTALVVFSTHAFTVTPLTTDTRTIASLVS
                     AVDTNIMPTQGGSLAAGMMKAAMLLEQTGLTRGDILLITDSEVAPEDVDLAGDLAGDG
                     YRVSVLAVGTDQGAPIPQPEGGFVTDSNGQVVIPQLDAPSLQRLAATGGGRYAALAPN
                     DRDLDTLFPAASPLDVTLEEAAGEQYEADVWLDRGLVLVVVLLPLLALSFRRGWICVF
                     ALVFLAPLPRAQAFEWQDLWQRPDQRGVRALEAQEAQRAAELFENPEWRSAAQYRAEQ
                     FEASAASLTNIDSAEGHYNRGNALAKAGQLPAAIAAYDRALELNPGHEDARYNRDLVE
                     QFLRDNPEQQQQPQPQEQQGDQNQQGDSDQSQSQSGEQQDGEQSSDEQSQQGQQGESG
                     DSQANDGAQEQQEPSDSEQQADDNAEQDANAGEQDQQQASNAAGPDDVEQWASEQAAE
                     QWLRRVPQDPGGLLRRKFLYQYQRMGVDQDGNRVEGAGAERRPW"
     gene            complement(4550..5596)
                     /locus_tag="VM692_09390"
     CDS             complement(4550..5596)
                     /locus_tag="VM692_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUQ52423.1"
                     /translation="MLTLAWWWMLLALPLPWLVRRFMAPEPLDRDAALKVPVAAEFAD
                     LSGLGAPDAARRWRLAVLAIVWLLAVLAAARPQLVGEPVALPMSGRDLLLSVDLSGSM
                     EEQDFQLNGEWVDRLTALKSVATDFIDRRVGDRVGLILFGREAYLQAPLTFDRATVST
                     LLDESVIGLAGKETAIGDSIGLAIRTLEDAGVEQGRRVLLLLTDGANTAGAVEPLKAA
                     ELAAQRKLVIYTIGIGADSLTVRSLFGLREINPSADLDEETLTAIAEMTGGRYFRARD
                     TTEFAEIYKLLDALEPAESDERGFRPITELFYWPLGAAVVVALAAALVGFAATRWQWS
                     RAALDRWQGGLHHG"
     gene            complement(5600..6133)
                     /locus_tag="VM692_09395"
     CDS             complement(5600..6133)
                     /locus_tag="VM692_09395"
                     /inference="COORDINATES: protein motif:HMM:NF025674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HUQ52424.1"
                     /translation="MNQTPDYSQLPLRDIHLPGAIGWWPPAFGWWLVAALVLAGFAAY
                     AVYYYRGRHKRAALRALTRVRAALEQGAEPVACLQYVSTILRRFAMTSVGAAPVTDAA
                     VDVPGLIGERWLHYLDGRWERTEFSAGLGRQLLVAPYARPNSIERAAAIELTGLCSAW
                     LTAQRATGTSTKRARAA"
     gene            complement(6130..>6796)
                     /locus_tag="VM692_09400"
     CDS             complement(6130..>6796)
                     /locus_tag="VM692_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUQ52425.1"
                     /translation="ERNRPVFVWLDLRRPMLFATRGAYKGVRAAELAALVAWSAVANG
                     DRLGGLVFSETEHHEVRPALGSRAALHLLQTVCTPSFWQPPTTAAALETDAERALQRL
                     TRVARPGSLIFLLSDFRRLGADHERHLRQLAMHCDLLLVHCYDAVEAELPPPGRYRIE
                     SLGRTFSIETAHEAMRRRYQERFAQRRGALEALCRMPGIRLVDCPTDAEPRSVLNQKF
                     RSR"
BASE COUNT         1104 a         2342 c         2322 g         1028 t
ORIGIN      
        1 ggcggatctg ctcggagacg ctctgcgcgt actcgtcggg cggcccgtac aacacgagca
       61 cgaactcttc gccgccgtag cgtgccgcaa aatcgaacgg gcgtttcgcg ccgcgggaga
      121 tgcacttcgc gacgcgcttc aaacagtcgt cgccggcctg gtggccgtaa aggtcgttgt
      181 agaccttgaa gaagtcgata tcgacgaaaa tgatcgcgat gacggtccgc tcgcggcgcg
      241 actggcgcca gagccgctcc atgtagtcgt cgaagatgcg acggttgtag aggctcgtca
      301 tgccgtcgcg ctcggcaagc tcgttcagga ggcgcgtctc caagaagttc aagcgcaacg
      361 cgtactcgag gttgtacgtc gagatgccgc cgatgaccgc ggcgccgccc aagatcgccg
      421 tgctgtagta cagcgcgtcg aacggcaagc cgatcgcgac gccggtgacg aagtgcgcgg
      481 cgatcatgac gcccgtcacg gtgaccgcga cgttgaacaa gagcccgagg aacaagcacc
      541 cgtagagcac gacgagcacg agaccggcaa gcacatacga cgagccggcg agcgcgccga
      601 gatgtgcgat gtacgtgacg acgaagccga tcagcgtgac gccgactgcg acgacctcgg
      661 tgtaatagcg ttgcgccgaa ggcaccgaga tcgcgactcc gagcacgacg agcagcggac
      721 agaggatgcc gagccgcagc gtgttcagcg tgccggcggt gttcgtgccg agcagcaggt
      781 cgatgcacgt cacggacagc acgacgatca gtgcgatgag ccccgacagg cgcgcgcgcg
      841 gcagattctg cgcgatgtag aactcgcgga agtctttctc caacaggcca ttgaagcgca
      901 ggccccgaaa cccgcgcttg agctgctcgg catacggcga cccgtagctg ttcgaatcga
      961 gcctttgtag atccataaaa cggtggcaac actgccggat tcggcagcat gcgctcaagg
     1021 cgcgcgggaa ttcttcaatc tagatcacaa acgccatcgt ttctcatcgg tacagcggta
     1081 gcaaaggatc gccggcgggt ggcgcgggcg ccgtccccgc gttttcgagc tcgcctaggg
     1141 cggctttgag cccctcgccg cgccatgcgc ccgccgccgc gccgtagatg tgcgcttcga
     1201 gcgcgagcac ttcgcgggcc gcgccgttcg gcagcagtgc cgccagcgct ccgaggctcc
     1261 gcggcggatt gtccgtgaac cgcgtctcgc cgagcttcag cagcgcttga cgcgccgcct
     1321 cgggatcgtt gacggcgcac gcgctctcaa ggtcgcgcag gatcttgcgc agcggctttg
     1381 gcgtcgccgc cggcggcgac ttcgtggcga cgggccgcgg cgcgcggccg cggttacgcc
     1441 accagagcgc gacggtcgcg atccaagcca gcgccagaac gccgctgatc cagggccacg
     1501 gattgcggtc gcgggcggca acaggcgacg gcgccgcggc gggcgcggcg gccggctccg
     1561 ctgcctctgc gtcggtgctc ggcgtgatgc gcaacggcag tggcgccaac tcggcgactt
     1621 cccagcgctg cgtcgcgacg ttgaaccacg gcaaatggac gccggccaac gtgacatcgc
     1681 ccggagtctg cgcgatcacg gcgtagccga cggtgcgccg cgacagcaat ccgtcggccg
     1741 tgatctcgcg cgtgagctca ggccgatcgg cgtattggcg aatgccggcc tgagtttcga
     1801 gcagaacgtc cggcagctgc gtctcgagca gacccagcgc ctcgacgccg atcgtgcgcg
     1861 tgcgcggaat gccgaccgcg agctcgtcgc cgggctcgct ccactgctcg gtcagcttga
     1921 cgctttgcgc cggcagccac gcagcacccg cgagcgccgg cggcggcgcg acggccggtt
     1981 gcaccgtgag ctccagcacg tctgagcgaa agcgctgcac gcgagagaag cctcgatccg
     2041 gaatcaccat tgcttcgaac gtcacgggcc cgacggtcag cgttccggcc tgctgcggaa
     2101 acaccgcgta gcgccgctcg cgaacgatga agtcgcggcc gccgcgactc gtctggtact
     2161 ggctgtcttc gccgagcttc tcgacgatcg cttcgacgcc cgtggtttca ggcgccgtca
     2221 gcgtcgcgcg gccagtgctg acgccgacga acaggcgcag cgtgaacagc acttgcgact
     2281 gcacgtagat gacgtcgggc tcggcgctga gctccatgaa gatgtcggcc gccgcggagg
     2341 cgttgggatc gggctccagt acgcgtacgg gcacggagtt cgactggcga tcgccgatgc
     2401 gcagcggcgg aatcgtgaag tcgccggcct tcttcggcat cagctggtat tgccactcgt
     2461 tgacctcgct tgcgcgcgcg ttgacgacgc cgatgcggcg gctgctcgtg gtgttcaaga
     2521 tgtcgaactg cgcggccagc ggcgcgatct cgggctcgcc gcgcacggcg ccttcggcgc
     2581 gcagcacgta cgtgaacgac tcgttgatgc gcaccacggc acgatcgacc gtcgcttgga
     2641 tcgccggctc ttgggcgaaa gccagcgcgc tcgcgagcgc gagccatgcg cagagcgccg
     2701 tcgctgcggc tttagcccat cccctcacca cggccgccgc tccgcgccgg cgccttcgac
     2761 gcgattgccg tcctgatcga cccccatgcg ctggtattga tagaggaatt tgcgccgcaa
     2821 caagccgccc gggtcctgcg gcacgcgccg taaccactgc tcggccgcct gctccgacgc
     2881 ccactgctcg acgtcgtcgg gcccggcggc gttcgacgcc tgctgttggt cctgctcgcc
     2941 ggcgttcgca tcttgctcgg cgttgtcgtc tgcttgctgc tcgctgtcgc tcggctcctg
     3001 ctgctcctgc gcgccgtcgt tggcctggct gtcgccgctc tcgccttgct ggccctgctg
     3061 gctctgctcg tcggaggatt gctcgccgtc ttgctgctca ccgctctgac tctgcgattg
     3121 atcggagtcg ccctgttggt tttggtcgcc ttgttgctct tgcggctgcg gctgttgctg
     3181 ttgctcgggg ttgtcgcgca agaactgctc gacgaggtcg cggttgtagc gtgcgtcttc
     3241 gtgacccggg ttcagctcga gcgcgcgatc gtaggcggcg atcgccgccg gcaactggcc
     3301 cgctttcgcg agggcgttgc cgcggttgta gtggccctcc gccgaatcga tgttcgtgag
     3361 cgaagcggcg ctcgcctcga attgctcggc gcgatactgc gcggcgctgc gccactcggg
     3421 attctcgaac agctccgccg ctcgctgcgc ctcttgcgcc tcgagcgcgc ggacgccgcg
     3481 ctgatcgggc cgctgccaca agtcctgcca ctcgaacgcc tgcgcgcgcg gcagcggcgc
     3541 gaggaacacc agcgcgaaaa cgcagatcca gccgcggcgg aacgagagcg ccagcaatgg
     3601 cagcaacacc acgacgagca cgaggccgcg atcgagccag acgtcggcct cgtattgctc
     3661 gccggcggcc tcctcgagcg tcacgtcgag aggcgacgcg gcaggaaaca acgtgtcgag
     3721 atcgcgatcg ttcggcgcga gcgcggcata gcggccgccg cctgtcgccg caagccgctg
     3781 caagctcggc gcatcgagct gcggaatcac gacttggccg ttcgagtccg tcacgaagcc
     3841 gccctcgggt tgcgggatcg gtgcgccttg gtccgtgccg acggccagca cgctgacgcg
     3901 gtagccgtca ccggcgagat cgcccgcgag atcgacgtct tcgggcgcaa cctccgagtc
     3961 cgtgatcagc aggatgtcgc cacgcgtgag ccctgtctgc tcgagcagca tcgcagcctt
     4021 catcatgccc gccgctaggc tgcccccttg cgttggcatg atgttcgtgt cgacggcgct
     4081 gacgagcgac gcgatcgtgc gcgtgtccgt cgtcaacggc gtgaccgtga acgcgtgcgt
     4141 cgagaacacg acgagcgccg tttgaccggc ggcgcggcgc tcgagcaagt cgagcagctt
     4201 cagcttcgcg cgtgcgagcc gcgacggctc gacgtcggcg gcgtccatcg accgcgacag
     4261 atcgagcgcc acgacgagcg cctcgtcgga gcgaaatgcc ggcacgggca gccgttccca
     4321 cgccggcccc gcgaggccga cgacggcaag cacccatgca gccagcgccg cgagcaaggc
     4381 gagccggctg tcgcgcaaca cttcgggctc ggccagcacg tgcggccgca agctcgcgtc
     4441 gacgacggtg cgccagccgc cgccgtcggc acggccgcgc agcagttgcc agatcagcca
     4501 ggcgccgacg ggcgcgagca gcagccagta agggcgcaag aagtggaact cagccatggt
     4561 ggagcccacc ttgccagcga tcgagcgcag cgcgcgacca ttgccagcgc gtcgccgcga
     4621 agccgacgag cgccgccgca agcgccacca cgacggcggc gccgagcggc cagtagaaca
     4681 gctccgtgat cggccggaag ccgcgctcgt cggactcggc cggctcgagc gcgtccaaga
     4741 gcttgtagat ctccgcgaac tccgtcgtgt cgcgcgcgcg gaaatagcgg ccgcccgtca
     4801 tctcggcgat cgccgtcaac gtctcctcgt cgagatcggc cgagggattg atctcgcgca
     4861 agccgaacaa cgagcgcacg gtcagcgaat cggcgccgat gccgatcgta taaatgacga
     4921 gcttgcgctg cgcggcgagc tcggcggcct tgagcggctc gacggcgccg gccgtgttcg
     4981 cgccgtcggt caagagcagc agcacgcgcc ggccctgctc gacgccggca tcctcgagcg
     5041 tgcgaatcgc gaggccgatc gaatcgccga tcgcggtttc cttgcccgcg aggccgatca
     5101 cggactcgtc gagcaacgtg ctcacggttg cgcgatcgaa ggtcaacggc gcctgtagat
     5161 acgcttcgcg gccgaatagg atgaggccga cgcgatcgcc gacgcgccga tcgatgaaat
     5221 ccgtcgcgac ggatttcaga gccgtcagcc ggtcgaccca ttcgccgttc agctggaagt
     5281 cctgctcctc catgctgccc gagaggtcga cggacagcag caagtcgcgg ccggacatcg
     5341 gcaacgcgac gggctcgccg accaactgcg gccgcgcggc tgcgagcact gcgagcaacc
     5401 agacgatcgc caacacggcg agacgccagc ggcgcgcggc gtccggtgcg cccaagcccg
     5461 agaggtcggc gaactcggca gcgacgggga ccttcagcgc cgcgtcgcgg tcgagcggct
     5521 cgggtgccat gaagcggcgc acgagccatg gcaacggcag cgccaacaac atccaccacc
     5581 acgccaacgt caacatggct tacgccgccc tcgcccgctt cgtgcttgtg cccgtcgcgc
     5641 gctgcgccgt cagccaggcg ctgcagagcc ctgtgagctc gattgccgcg gcgcgctcga
     5701 tgctgttcgg gcgcgcgtac ggcgcgacga gcagttggcg tccgagccct gcgctgaact
     5761 cggttcgctc ccaacggccg tcgagatagt ggagccaacg ctcaccgatg agccccggca
     5821 cgtcgacggc ggcgtccgtg acgggcgccg cgccgaccga cgtcatcgcg aaacgccgca
     5881 ggatcgtcga gacgtattgc agacaggcga ccggctcggc gccctgctcg agagcagcgc
     5941 gcacgcgcgt cagggcgcgc aatgccgcgc gcttgtggcg gccgcggtag taatagacgg
     6001 cgtaagcggc aaagcccgcc agcacgagcg ccgcgacgag ccaccagccg aacgccggcg
     6061 gccaccagcc gatcgcgccc ggcaagtgaa tgtcgcggag cggcagctgc gaatagtcgg
     6121 gcgtctggtt catcgtgacc gaaacttctg gttcaacacg ctgcggggct cggcatcggt
     6181 cgggcagtcg acgaggcgga tgcccggcat tcggcatagc gcctcgagcg cgcctcggcg
     6241 ctgcgcgaat cgctcctggt agcggcgccg cattgcctcg tgcgccgtct cgatgctgaa
     6301 cgttcgcccc aagctctcga ttcgatagcg ccccggcggc ggaagttcgg cctcgactgc
     6361 atcgtagcaa tgcacgagca gcaaatcgca atgcatggcg agctggcgca ggtgccgctc
     6421 gtggtcggcg ccgaggcgac ggaaatcgct gagcaggaag atcaagctgc cgggccgcgc
     6481 gacgcgcgtc aaacgctgca acgcacgttc ggcatccgtc tcgagcgccg cggccgtcgt
     6541 cggcggctgc cagaacgacg gcgtacagac ggtttgcaga agatgcagcg ccgcgcgcga
     6601 gcccaacgcg ggccgcacct cgtggtgctc ggtctccgag aacacgaggc cgccaagtcg
     6661 atcgccgttc gcgacggcgc tccaggcgac gagcgcggcg agctcggcgg cgcgcacacc
     6721 tttgtatgcg ccacgcgtcg cgaacagcat cggccggcgc aggtccagcc agacgaacac
     6781 gggccggttg cgctct
//
LOCUS       DATLEE010000439         2007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3238897, whole genome shotgun sequence.
ACCESSION   DATLEE010000439 DATLEE010000000
VERSION     DATLEE010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3238897"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VM692_09405"
     CDS             <1..1083
                     /locus_tag="VM692_09405"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ52426.1"
                     /translation="LSGLAHLEADTKGDGNGDHPRASAVWLSGVHAWDRTKPGIDVRL
                     ATTADQLAAREIGKGTPVASLELNVDNPTQGSCDSGDCFYVNTVSWRNETTPNLTENH
                     PRVVFERLFGDGGSNAERLARTRKTASLLDSVRGEASRLAASVGHSDRSKLSEYLDSV
                     REIEQRIGAAEAEGHGDVELPERPLGIPDSFDDYAKLMLDLQLLAYRTDTTRVFSLIL
                     AREVSNRPYPQIGVPDQHHAASHHRNDGGLIEKKTKIDAYHISLLGYLADKMRTTSDG
                     DGTLLDQSLLVYGGGMGDGNLHQHVDLPVVLLGKLGGTIKAGQHVAYPAGTPMTNLLV
                     TVLDKVGVAVDTIGDSTGRLDYLTAV"
     gene            1249..>2007
                     /locus_tag="VM692_09410"
     CDS             1249..>2007
                     /locus_tag="VM692_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HUQ52427.1"
                     /translation="MLPSLRLLSLAVVVTLAMNAKADAVAANDARRDVDLVIALDVSG
                     SMEGLIESAKQRLWDVTNELAQARPAPNLRVAILSYGKPGYGEQAGYVHVDQPFTSDL
                     DAVNATLFAFVTDGGDEYVARAIQTSLDVLDWSKDTNALQIVFVAGNESVEQDPRLTV
                     ERATAEAARRGIVVNAIYCGGDGDEDARAWRRLATSTNGMYASIDQNAAAVANVATPF
                     DAPLAALNDEMNKTYIAFGTAGERGRANQAEQDRN"
BASE COUNT          390 a          660 c          636 g          321 t
ORIGIN      
        1 ctgagcggcc tggcacatct cgaagccgac accaaaggag acgggaacgg cgatcatccg
       61 cgcgcgtctg ctgtctggtt gtccggagtt cacgcgtggg atcgaacgaa gccgggtatc
      121 gacgtccgtc tggcgaccac ggcggatcag cttgccgcac gtgagatcgg caaaggcacg
      181 cccgtggcgt cgctggaact gaacgtcgac aatcccacac agggttcgtg cgattccggc
      241 gactgctttt acgtcaacac cgtttcctgg cgcaacgaga cgacgccgaa cctcacggag
      301 aaccatccgc gcgtcgtatt cgagcggctg ttcggcgacg gcggcagcaa cgccgagcgc
      361 ctcgcgcgca cgcgcaagac ggcgagcctg ttggactccg tgcgcggcga agcgagccga
      421 ctcgctgcaa gcgtcgggca cagcgaccgc agcaagctca gcgagtactt ggactccgtg
      481 cgcgagatcg agcagcgcat cggtgcggcc gaggccgaag ggcacggcga cgtcgagctt
      541 ccggagcggc cactcggcat cccggactcg ttcgacgatt atgcgaagct gatgttggac
      601 cttcagttac ttgcctatcg tactgataca acacgtgttt tcagcctgat actcgcgcgc
      661 gaagtcagca atcgcccgta cccgcagatc ggcgttcccg atcagcatca cgcggcttcg
      721 caccatcgca acgatggggg cttgatcgag aagaagacca agatcgatgc gtaccacatc
      781 tcgctgctcg gctacttggc cgacaagatg cgcacgacct ccgatggcga cggcacgctg
      841 ctcgaccaga gcttgctcgt ctacggcggc ggaatgggcg acggcaacct gcaccagcac
      901 gtggatttgc cggttgtcct gctcggcaag ctcggcggca cgatcaaggc gggccagcac
      961 gtcgcctacc ccgccggcac gccaatgacg aatctgctcg tgacggtgct cgacaaagtc
     1021 ggcgtcgccg tcgacaccat cggcgacagc acggggcgac tcgattacct gaccgccgtc
     1081 tgaggacgcc accggctccg ccgcgagctt cgcaccattc gcagcctccg gtgacggtct
     1141 tgtaaagcgc gagtaaaaat cgctcgccat cttttacgtc gcgtgtctgg aagccgtggc
     1201 acaacgccgc gcagcatctg ctcatccttc attcaatagg agacgacgat gcttcccagc
     1261 ctgcgcttgc tctctctcgc cgtggtcgtg acgctcgcca tgaacgccaa ggccgacgcc
     1321 gttgcggcca acgatgcacg gcgcgatgtg gacctcgtca tcgcgctcga cgtcagcggc
     1381 tcgatggaag gcttgatcga gtcggcgaaa caacgcctct gggacgtcac gaacgagctc
     1441 gcgcaagcgc ggccggcgcc gaacctgcgc gtcgcgatct tgagctacgg caagcccggc
     1501 tacggcgagc aagcgggcta cgtgcacgtc gaccagccgt tcacgtcgga cctcgatgcc
     1561 gtcaacgcga cgttgttcgc cttcgtgacg gacggcggcg acgagtacgt cgctcgcgcg
     1621 atccaaacgt cgctcgacgt cctcgattgg agcaaggaca cgaatgcgct gcagatcgtt
     1681 ttcgtcgccg gcaacgaatc cgtggagcaa gacccgcggc ttaccgtcga gcgcgcaacg
     1741 gccgaagcgg ctcggcgcgg catcgtcgtc aacgcgattt attgcggcgg cgacggcgac
     1801 gaagacgcgc gcgcatggcg gcgcctcgca acgagcacga acgggatgta tgcgagcatc
     1861 gaccagaacg cggcggccgt cgcgaacgtg gcaactccgt tcgacgcgcc gctcgccgcc
     1921 ttgaacgacg agatgaacaa gacgtacatc gcgttcggca ccgcgggaga gcgcgggcga
     1981 gccaaccaag ccgagcagga tcgcaat
//
LOCUS       DATLEE010000440         8905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5708221, whole genome shotgun sequence.
ACCESSION   DATLEE010000440 DATLEE010000000
VERSION     DATLEE010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5708221"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3530
                     /locus_tag="VM692_09415"
     CDS             <1..3530
                     /locus_tag="VM692_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HUQ52428.1"
                     /translation="DGTYRLQGAPAQAASAPTSVQVPPDIDVLVRNSRVLYLDAARTV
                     AWDFQDVAASLRRVDEIVTLQASARPPAEFAERIELTAQASVADGDGGAKFTGDWRLS
                     ADVDEADLAVAARLFPPSAVAPQAGRGDVAVWLEWQNGALASGTAELALADVALRSSL
                     GAVDSRFERIALSGTWQHNADDWHFALRDVAVTRAGRAWPEAATVDIDVTRDADGVDH
                     FGLRSSFLRLEDLTPFFAPLPESRLLESWFTLAPRGDLRGVDVALSRNVDERIDYTVA
                     AEFAGLGVAKFGDWPGVTQLTGQVRADSRAGRIELASEGAALDWPSLFRDAIDLPEVR
                     GIVVWRAGQDAVRVVSDDLLVATPDASLRANLELTLPMDDRSPELDLRMGASGFDIAA
                     ARRYLPAGKMPPTVVGWLDSALRGGRATSAELTFVGPVRAFPFDGGEGELHATVNVED
                     AALAFVSDWPLAEDLDGTLEFFNSRFTGRGSGRMLGQRTADVRVSIGDLRQGDLAVQA
                     NTIGGLDQVLAFLNGAPLIARYLGEDFARLEAPSGSGAVSFDLALPLRNREAFRLTAG
                     LDIIDGELVYEGFAPHVTEIHGSLALADGVLTGQNIQAIFLDGPITAKVGAGGVPGYR
                     SRIDAEGEVTIDAVVDAFSLPYGERMAGQTAWQGTLLLPAAAGAQSLPAKISVTSNLS
                     GVALRFPQPFAKPPGEPTNLELNIAFPEGALAMDGYFGATRRFALDFDATPGAAKPFQ
                     FRRAALQFGGALAEYRSERGVTLNGSLPLLEVDDWLALSRTDTSAVPAATGRGANWSG
                     VFAGADLNVAEFSVFGQRLGSTRLSARRRTDDWQFDLDSNAIAGTLLVPLDLSGDPQV
                     VAVMRRLYLNAGDGAGGRGTSGLDPRELPGLQLHADEFGVGQRQIGRLDAEVLADPLG
                     LRLVSFESATDSFTAQGSGGWFVGDEGDTTRFAVSINSTNVGQMLEQIGVARFVEAET
                     AEVTASVFWPGPPSGAWLDHIGGDMALRATKGSLIDVEPGGAGRAAGLLSISALPRRL
                     ALDFRDVFNRGLVFDDVTADFVVVDGNAYTDNLKLTGPVAEVGLIGRTGLRDRDYRQQ
                     AIVTAEPGKVLPTVGALLAGPQVAAALLIFTRIFKKPLGGIGRASYCVTGTWGEPMVE
                     RLTNEQLEEGALCVELPPNGMPPKPAEVG"
     gene            3527..4336
                     /locus_tag="VM692_09420"
     CDS             3527..4336
                     /locus_tag="VM692_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HUQ52429.1"
                     /translation="MKVAAVQMTSTRDVQANLREAGRLVADAAKQGAKLVVLPENFSF
                     LGATDADRVAAVERFGDGPAQRFLAETAEELGLWIVGGTIPIKDAGERASSRSLLVGP
                     DGRIAAHYDKIHLFDVDIPTKTAERYMESATTLAGTRVVAAETPLARIGMTVCYDIRF
                     PALFHRLSVLGTDIVVVPAAFTVPTGEVHWLPLLQARAIESLVYVVAAGQWGEHAGGR
                     KTYGHSLILGPWGERLGELAAGPGVVCADLDMIRLQELRQRFPTVQHRREL"
     gene            4348..5823
                     /gene="tldD"
                     /locus_tag="VM692_09425"
     CDS             4348..5823
                     /gene="tldD"
                     /locus_tag="VM692_09425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HUQ52430.1"
                     /translation="MAIPSQSAVTAADSFAIARELLLEGAGLELAALDRALATAMVRK
                     LDYVDLYLQLTRYETWTVEDGIVKEGAYSIDQGIGVRAISGERTGFAYSDQLDEAALL
                     DAVAAARGIARTQGEGSQRIAARPASHRPLYPSADPLHSMSDSDKVALLADLDRRVRG
                     MDNRVAQVIASLAGVYEVVLVQATDGTLAADVRPLVRLNVSVIMEKDGRREQGYAGGG
                     GRGDYTLLTAAGRPLEIAAEAVRTAAVNMSAVPSPAGNMPVVLGSGWPGILLHEAIGH
                     GLEGDFNRKGTSAFAGRVGERVASEQCTVVDDGTLLGRRGSLTVDDEGTPTRCNTLIE
                     NGVLKGYMFDKMNARLMNQKSTGNGRRESFAHTPMPRMTNTYMLPGTYDPEEIIASVD
                     KGIYAPNFGGGQVDITSGKFVFSASEAYLVENGKKTVPVKGAMLIGNGPEVLTRVSMV
                     GNDLKLDAGLGTCGKDGQSVPVGVGQPTIRIDSLTVGGTQA"
     gene            5861..6091
                     /locus_tag="VM692_09430"
     CDS             5861..6091
                     /locus_tag="VM692_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52431.1"
                     /translation="MNSFTAIDEAAPYAARMTIDFSSTMTEADFKRPLLDAAEARLAL
                     EARGEVDLAVEGLKDSLLSRLVRLVSGGGARS"
     gene            6105..6299
                     /locus_tag="VM692_09435"
     CDS             6105..6299
                     /locus_tag="VM692_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52432.1"
                     /translation="MWLSKPVYEALPYYYGALGVVALLARLYVDYWYWPWICTIVGVG
                     SLAAGVLVWLKRRGHRSRAS"
     gene            complement(6304..7629)
                     /locus_tag="VM692_09440"
     CDS             complement(6304..7629)
                     /locus_tag="VM692_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUQ52433.1"
                     /translation="MYCELFKLTEPPFRLTPDPQFLFASKQHARAKAYMESTIWLADG
                     FVVITGDIGSGKTTLIESFLTDLPPDIILAHISQTQLTPVEFLQALLVEFGFKPFRKR
                     KVELLSMLKDYMVEQYAAGKKILLIIDEAQNLSRKVLEEVRLLSGLEAQKEKLLRIIL
                     AGQPELANKLDSPRLQQLVQRVRLRFHLGALSKRETHEYISHRLKVAGAGDREIFQTT
                     ACDVVFRYTGGVPRLVNVLCDTAMLCAFAEERTVVDEALVKAAVEELQWVEFSERLRA
                     QSRHGDHTGRFGNPADRPMGRLEIMFRDQLVGEHRLGFGKTIIGRTPDNDVQIQSRFI
                     SRHHAQVVSDETQSILEDLNSTNGVFIRAQRVKHQVLADGDVIQLGEHKLLYRDMRGM
                     AAPRVAVEDDDEDEDDDDDRDDDFEDDEDELDDDDADERDEQQVDERRS"
     gene            complement(7705..8805)
                     /locus_tag="VM692_09445"
     CDS             complement(7705..8805)
                     /locus_tag="VM692_09445"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HUQ52434.1"
                     /translation="MTRPRVGIVGAGQLGRMLALAGYPLGIDCTLLDTGDDSPGAQVA
                     DFVAGRLGDPAALAKLADKVDVLTLEIENVAVAALESLHGRIDVFPPPGAVAAAQDRL
                     AEKTLFRSLGIPTAEFVTIDSEADAAAAERLGWPAIVKTRRMGYDGRGQRVVDSTAAL
                     AAAWRELGGVPSIAEAFVEFEREVSLIGVQGARDERVFYALAENVHRNGILASTVAPY
                     DDHDLQQQAEAWLTAIMAKLEYRGVLTVEFFHTTDGLVANEMAPRVHNSGHWTIEGAA
                     TSQFENHLRAVLGWPLGDPSVRGHAAMLNLLGRLPPRDAVLRIAGAHLHDYGKSPRPG
                     RKVGHCTIVDAQRAKLLERLEPLRDLVFRSNQ"
BASE COUNT         1371 a         2968 c         3093 g         1473 t
ORIGIN      
        1 aggacggcac gtaccgcttg cagggcgcgc cggcgcaggc ggcgagcgcg ccgacttccg
       61 tgcaagtgcc gcccgacatc gacgttctcg tgcgcaacag ccgcgtgctg tacctcgacg
      121 ccgcgcgcac ggtggcttgg gacttccaag acgtcgccgc gagcctgcgc cgcgtcgacg
      181 agatcgtgac gctgcaggcc agcgctcgcc cgcccgcgga gttcgcggag cgcatcgagc
      241 tgacggcgca agcgtcggtc gccgacgggg acggcggggc gaagttcacg ggcgactggc
      301 gcctctcggc cgacgtcgac gaggccgatc tcgccgtagc ggcgcgattg ttcccgccat
      361 cggccgtcgc gccgcaagcg ggccgcggcg acgtggccgt ctggctcgag tggcagaacg
      421 gcgcgctcgc gagcggcacc gcggagctcg cgctcgccga cgtggcattg cggagctcgc
      481 tcggcgccgt cgattcgcgt ttcgagcgca tcgccttgtc gggcacctgg cagcacaacg
      541 cggacgactg gcatttcgcg ctgcgcgacg tcgccgttac acgcgccggc cgcgcttggc
      601 ccgaggcagc gacggtcgac atcgacgtca cgcgcgacgc cgatggagtc gaccatttcg
      661 gcctgcgcag cagcttcctg cggctcgagg atctgacgcc gtttttcgcg ccgttgccgg
      721 aatcgcgact gctcgagtcg tggtttacgc tggcgccgcg cggcgatctg cgcggcgtcg
      781 acgttgcgct gtcgcgcaat gtcgacgagc gcatcgacta caccgtcgcc gccgagttcg
      841 cgggtctcgg tgtggcgaaa ttcggtgact ggcccggggt cacgcagttg acgggccagg
      901 tccgagccga ctcgcgggcg ggtcgcatcg agctcgcgag cgagggcgcg gcgctcgatt
      961 ggcccagcct gttccgcgat gccatcgact tgcccgaggt gcgcggcatc gtcgtctggc
     1021 gggcgggtca ggacgcggtg cgcgtcgtca gcgacgattt gctcgtcgca acgccggacg
     1081 cgtcgttgcg cgccaacctc gagctcacgc tgccgatgga cgaccgctcg cccgagctcg
     1141 acctccgcat gggcgcgtcg ggcttcgaca tcgccgcggc gcgccggtac ttgccggcgg
     1201 gcaagatgcc gccgaccgtc gtcggctggc tcgactccgc gctgcgcggg gggcgtgcga
     1261 cgagcgcgga gctcacgttc gtcggtcccg tgcgcgcgtt cccgttcgac ggcggcgagg
     1321 gcgagctcca tgcgaccgtc aacgtcgaag acgccgcgct cgcgttcgtc agcgactggc
     1381 cgctcgcgga ggacctcgac ggcaccctcg aattcttcaa ctcgcggttc acggggcgcg
     1441 gcagcggccg catgctcggg caacggacgg ccgacgtgcg cgtcagtatc ggcgacctgc
     1501 gccaaggcga tctcgcggtg caagcgaaca cgatcggcgg gctcgatcag gtgctcgcgt
     1561 ttctgaacgg cgcgccgttg atcgcgcgct atctcggcga ggacttcgcg cggctcgagg
     1621 cgccgagcgg cagcggcgcc gtgagcttcg atctggccct gccgctgcgc aatcgcgagg
     1681 cgtttcgttt gacggcgggg ctcgacatca tcgacggcga gctcgtctac gaagggttcg
     1741 ccccgcacgt cacggagatc cacggctcgc tcgcgctcgc ggacggcgtg ctgacgggcc
     1801 agaacatcca ggcgatcttc cttgacgggc ccatcacggc gaaggtgggc gccggcggcg
     1861 tgccgggtta tcgctcgcgc atcgacgcgg agggcgaggt cacgatcgac gccgtcgtcg
     1921 acgcgttcag cttgccgtac ggcgaacgca tggccggcca aacggcctgg cagggcacac
     1981 tgttgttgcc ggctgccgcg ggcgcgcaga gcctgccggc gaagatcagc gtgacgtcga
     2041 acctgtcggg cgtcgcgctg cgctttccgc agccgttcgc gaaaccgccc ggcgagccga
     2101 ccaacctcga gctcaacatc gcgttccccg agggcgccct cgcgatggac ggctacttcg
     2161 gcgcgacgcg ccggttcgca ctcgatttcg acgcgacgcc gggcgccgcc aaaccgttcc
     2221 agtttcggcg tgccgctttg cagttcggcg gcgcgctcgc ggagtatcgc tccgagcgcg
     2281 gcgtgacgct gaacggcagc ttgccgctgc tcgaggtcga cgattggctc gcgctgtcgc
     2341 gcaccgacac cagtgccgtg ccggccgcga cgggccgcgg cgcaaactgg agcggcgtgt
     2401 tcgccggggc cgatctcaat gtcgccgaat tttcggtgtt cggccaacgc ctcggcagca
     2461 cccgcttgag cgcgcgccgg cgcaccgacg attggcagtt cgacctcgac agcaacgcca
     2521 tcgcgggcac gctgctcgtg ccgctcgatc tgagcggcga cccgcaagtc gtcgcggtga
     2581 tgcggcggct ctatctgaat gccggtgacg gcgccggggg ccgtggcacg agcggtctcg
     2641 atccgcgcga gctgccgggt ttacagcttc acgccgacga gttcggcgtc gggcagcggc
     2701 agatcgggcg cctcgacgcc gaggttctcg ccgatccgct cgggcttcgc ctcgtgtcgt
     2761 tcgagagcgc caccgacagt ttcacggcgc aaggcagcgg cggttggttc gtcggcgacg
     2821 agggcgacac gacgcgcttc gccgtcagca tcaactcgac caacgtcggc cagatgctcg
     2881 agcagatcgg cgttgcgcgg ttcgtcgagg ccgagacggc cgaggtgacg gccagcgtgt
     2941 tctggccggg gccgccgtcg ggcgcgtggc tcgatcacat cggcggcgac atggcgttgc
     3001 gcgcgaccaa aggcagcctc atcgacgtcg agcccggcgg cgccggccgc gccgcgggac
     3061 tcttgagcat cagcgcgctg ccgcggcgcc tcgcgctcga tttccgcgac gtgttcaacc
     3121 gcgggctcgt gttcgacgac gtcacggccg acttcgtcgt cgtcgacggc aacgcctaca
     3181 ccgacaacct caagctgacg ggacccgtgg ccgaggtcgg tctcatcggc cgcacggggc
     3241 tacgcgatcg cgactaccgc cagcaagcga tcgtcacggc cgagcccggc aaagtgctgc
     3301 cgacggtcgg tgcgttgctc gcgggcccgc aggtcgccgc ggcgctgctg atcttcacgc
     3361 gcatcttcaa gaagccgttg ggcggcatcg gccgcgcgtc gtattgcgtg acgggcacct
     3421 ggggcgagcc gatggtcgag cggctgacca acgagcagct cgaggagggc gcgctgtgcg
     3481 tcgagttgcc gccgaacggc atgccgccga agcctgcgga ggtcggatga aagtagcagc
     3541 cgtgcaaatg acgagcacgc gcgacgtgca agcgaatctg cgcgaggcgg gccgactcgt
     3601 cgccgacgcc gcaaagcagg gcgcgaagct cgtcgtgctg cccgaaaatt tttcgttcct
     3661 cggcgcgacg gacgccgatc gcgtcgccgc ggtcgagcgt ttcggcgacg gtcccgcgca
     3721 gcggttcctc gccgagacgg ccgaggagct cggcctctgg atcgtcggcg gcacgattcc
     3781 gatcaaggac gccggtgagc gtgcgtcgtc gcgctcgctg ttggtcgggc ccgacggccg
     3841 aattgccgcg cactacgaca agatccacct gttcgacgtc gacatcccga cgaagacggc
     3901 agagcgctac atggaatcgg cgacgacact tgcgggcacg cgcgtcgtcg cggcagagac
     3961 gccgctcgcg cgcatcggca tgacggtctg ctacgacatc cggttcccgg cgctgttcca
     4021 tcgcctgagc gtgctaggca cggacattgt tgtcgtgccg gccgcgttca cggtgccgac
     4081 gggcgaggtg cactggctgc cgctgttgca agcgcgcgcg atcgagtcgc tcgtctacgt
     4141 cgtcgcagcc gggcagtggg gcgagcacgc cggcgggcgc aagacctacg gccattcgct
     4201 gattctcggg ccttggggcg agcgcctcgg cgaattggcc gccggtcctg gcgtggtttg
     4261 cgccgatctc gatatgatcc ggcttcagga gctgcgccag cgcttcccca cggttcaaca
     4321 ccgccgcgag ctctaggaga tttaccgatg gcgataccct cgcagtccgc agtgactgcc
     4381 gccgattctt tcgcgattgc acgcgagttg ttgctcgaag gcgcgggcct cgagcttgca
     4441 gcgctcgacc gtgcgttggc gacggccatg gtgcgtaagc tcgactacgt cgacctctac
     4501 ctgcagctga cgcgctacga gacctggacc gtcgaggacg gcatcgtcaa ggaaggcgcc
     4561 tacagcatcg atcagggcat cggcgtgcgc gcgatctcgg gcgagcgcac gggtttcgct
     4621 tattccgatc agctcgacga ggcggcgctg ctcgacgcgg ttgccgcggc gcgcggcatc
     4681 gcgcgtacgc aaggcgaggg ctcgcagagg atcgcggcaa ggcccgcgag ccatcggccg
     4741 ctctatccga gcgcggatcc gctgcacagc atgagcgaca gcgacaaggt tgctttgctc
     4801 gcggacctgg accggcgcgt ccgcggcatg gacaaccgcg tcgcgcaggt catcgcgagc
     4861 ctcgccggcg tttacgaggt cgtgctcgtg caggcgaccg acggcacgct ggccgccgac
     4921 gtgcggcccc tcgtgcggct caacgtctcg gtcatcatgg agaaggacgg ccggcgcgag
     4981 cagggctatg ccggtggcgg cggccgcggc gactatacgc tactgacggc tgccggccgg
     5041 ccgctcgaga tcgcggccga agccgtgcgt accgcggccg tcaacatgtc ggccgtgcct
     5101 tcgccggcgg gcaacatgcc cgtggtcttg ggctcgggct ggcccggcat cttgttgcac
     5161 gaggccatcg gccacggcct cgaaggcgac ttcaatcgca agggcacctc ggcgttcgcc
     5221 ggtcgcgtcg gcgagcgcgt cgcatcggag caatgcacgg tcgtcgacga cggcacgctg
     5281 ctcggccgcc gcggctcgtt gaccgtcgac gacgagggca cgccgacgcg ctgcaacacg
     5341 ctcatcgaga acggcgtgtt gaagggctac atgttcgaca agatgaacgc gcgcctcatg
     5401 aaccagaagt cgacggggaa cggccgccgc gagtcgttcg cgcacacgcc gatgccgcgc
     5461 atgacgaaca cgtacatgct gcccggcacg tacgatcccg aggagatcat cgcgtccgtc
     5521 gacaaaggca tctacgcgcc gaacttcggc ggcggccaag tcgacatcac gagcggcaag
     5581 ttcgtgttct ccgcgagcga ggcttatctc gtcgagaacg gcaagaagac agtgcccgtc
     5641 aaaggcgcga tgctgatcgg caacgggccc gaagttttga cgcgcgtttc gatggtcggc
     5701 aacgacttga agctcgacgc cgggctcggc acctgcggca aagacggcca atcggtgccc
     5761 gtcggcgtag gccaaccgac gattcgcatc gactcgctga cggtcggcgg cacgcaggcc
     5821 taacctttgc gtcggccttg aattaagtca aagtgcggtc gtgaatagct tcactgcaat
     5881 cgacgaggcg gcgccgtatg ctgcgcgcat gacgatcgat ttcagctcga cgatgacgga
     5941 agccgatttc aagcggccgt tgctggacgc ggccgaagcg cggctcgcgc tcgaagctcg
     6001 cggcgaggtc gacttggccg tcgagggatt gaaagactcg ctgctgagcc ggctcgttcg
     6061 gctcgtctcc gggggcggcg cccggagtta gactagacag ccatatgtgg ttgtcgaaac
     6121 ccgtctacga agctctaccg tactactacg gtgcgctcgg cgtcgtcgcg ctgctcgcgc
     6181 ggctctacgt cgactactgg tactggccgt ggatctgcac gatcgtcggc gtgggcagcc
     6241 tcgcggccgg cgtgctcgtc tggttgaaac ggcgcggcca tcggtcgcgc gcgagctgac
     6301 cgctcagctg cggcgctcgt ccacctgttg ctcgtcgcgc tcgtcggcgt cgtcgtcgtc
     6361 gagctcgtcc tcgtcatcct cgaagtcgtc gtcgcggtcg tcatcatcgt cctcgtcctc
     6421 gtcgtcgtct tcgacggcaa cccgcggcgc ggccatgccg cgcatgtcgc gataaaggag
     6481 cttgtgctcg ccgagctgga tgacgtcgcc atcggcaagc acctgatgct tcacacgctg
     6541 tgcgcggatg aacacgccat tcgtgctgtt caagtcctcc aaaatcgact gcgtctcgtc
     6601 ggacacgacc tgcgcgtgat gacgcgagat gaagcggctt tggatctgca cgtcgttgtc
     6661 gggcgtgcgg ccgataattg ttttgccgaa gccgaggcga tgctcgccga cgagctggtc
     6721 gcggaacatg atttcgagcc gccccattgg tcgatccgcc ggattgccga agcggccggt
     6781 gtgatcgccg tggcgactct gggcgcgcaa gcgctccgag aactcgaccc attgcagctc
     6841 ttcgacggcg gccttgacga gagcttcgtc gacgaccgtg cgttcctcgg cgaacgcgca
     6901 cagcatggcc gtgtcgcata gtacgttcac gagccgcggc actccgcccg tgtagcgaaa
     6961 cacgacatcg caagccgtgg tctggaagat ctcgcggtcg cccgcgccgg caaccttcaa
     7021 ccgatgagag atgtactcgt gcgtctcgcg ttttgataac gcgcccaagt gaaagcgcag
     7081 gcgcacgcgc tgcacgagct gctgcaagcg cggcgagtcg agcttgttgg cgagctcggg
     7141 ctggcccgcg aggatgatgc gcagcagctt ctccttctgc gcctcgagcc ccgacagcag
     7201 gcgcacttcc tcgagcacct tgcgcgacaa attctgcgct tcgtcgatga tcagcaggat
     7261 cttcttgccg gccgcgtact gctcgaccat gtagtccttg agcatggaca agagctcgac
     7321 tttgcgcttg cggaacggct tgaagccgaa ctcgacgagc agcgcctgca gaaactcgac
     7381 gggcgtcagc tgggtctggc tgatgtgcgc gaggatgatg tcgggcggca ggtcggtcag
     7441 aaagctctcg atcagcgtcg tcttgccgga gccgatgtcg ccggtgatga cgacgaagcc
     7501 gtcggcgagc caaatcgtcg actccatgta cgctttggcg cgcgcatgct gtttgctggc
     7561 aaacaggaac tgcggatccg gcgtcagccg gaacggcggt tcggtcagtt taaagagctc
     7621 gcaatacatg cggacttcgc gttcggactc gtgggtggaa ggtcggagac taccggaaag
     7681 gtcaaggtgt ttcgagggac ggaatcactg attcgagcga aaaaccaagt cccgcaacgg
     7741 ctccagacgc tcgagtagct tggcgcgctg cgcatcgacg atcgtgcagt gcccgacttt
     7801 gcggcccggc cgcgggctct tgccgtagtc gtgcagatgc gcgcccgcaa tacgcagcac
     7861 ggcgtcacgc ggcggcaagc ggcccagcaa gttcagcatc gcggcatggc ctcgcacgga
     7921 cggatcgccg agcggccaac cgagcaccgc acgcaggtga ttctcgaact ggctagtcgc
     7981 ggcgccttcg atcgtccagt gccccgagtt gtggacgcgc ggcgccatct cgttggcgac
     8041 gagcccatcg gtcgtatgga agaactcgac ggtcaacacg ccgcgatact cgagcttggc
     8101 catgatagcg gtgagccatg cttcggcctg ctgctgtagg tcgtggtcgt cgtacggcgc
     8161 gacggtgctc gccaagatgc cgttgcgatg cacgttctcg gcgagcgcgt agaacacgcg
     8221 ctcgtcgcgc gcgccttgca cgccgatcaa cgaaacttcg cgctcgaact cgacgaatgc
     8281 ctcggcgatc gacggcacgc cgccgagctc acgccatgcg gccgcgagcg ccgccgtgga
     8341 atcgacgacg cgttgaccgc gaccgtcata gcccatgcgc cgcgttttta cgatcgcggg
     8401 ccagccgagt cgctcggcgg cggctgcgtc cgcctccgag tcgatcgtca cgaactcggc
     8461 cgtcggaatg ccgagcgagc ggaacaatgt tttttcggcg agccggtcct gcgcggcggc
     8521 gacggcgccc ggcggcggaa agacgtcgat gcggccgtgc agcgactcga gcgcggcgac
     8581 ggcgacgttc tcgatctcga gcgtcaggac gtcgaccttg tccgcgagct tcgcgagcgc
     8641 ggcgggatcg ccgagccggc ctgcgacaaa gtccgcgacc tgcgcgcccg gcgagtcgtc
     8701 acccgtatcg agcagcgtgc aatcgatgcc gagcggatag cccgccagcg cgagcatgcg
     8761 tccgagctgg cccgcgccga cgataccaac tcggggtcgc gtcatggact cgcgcgccgc
     8821 ccgtcagcga gcgctgcgcg gatccggctg ctcgagcacc tcggccgttt gcttggcgcg
     8881 aaactgctct agcgcggtgg cgacg
//
LOCUS       DATLEE010000441         5939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2728637, whole genome shotgun sequence.
ACCESSION   DATLEE010000441 DATLEE010000000
VERSION     DATLEE010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2728637"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..678)
                     /locus_tag="VM692_09450"
     CDS             complement(94..678)
                     /locus_tag="VM692_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52435.1"
                     /translation="MSQDAELESWRRQWQGAAAPPADAVVQLRQRVLRETRWLKWSLI
                     GPILVTLGVGGGMTLRALRTQQPLDVLFAVETWIFIVVTWIGALWLARGTWRPLADTT
                     AAFVAVSIRRRKANARAAIFALCLYVAQLVFVVLALAAELPGGVARILTSSFMVVVGW
                     IGIPAGLALIYWFHRRRRAELERLRELERQLRTD"
     gene            complement(675..1220)
                     /locus_tag="VM692_09455"
     CDS             complement(675..1220)
                     /locus_tag="VM692_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUQ52436.1"
                     /translation="MTTTTAGGRQVAERAGDSLDERFRRLLEANHAPLTRLAASYAGN
                     RSDRDDLLQEIAIAVWRALPSLRGDCSERTFLFRIAHNRCMTHLSKRRPTVSLDEAQI
                     DVEDPNVSAETLLAEEQQRRRFVQAIRGLPAIHREVLVLFLEGMDYGEIAAVVGISES
                     NVGVRLNRARQRLKELLEEGT"
     gene            1344..2771
                     /locus_tag="VM692_09460"
     CDS             1344..2771
                     /locus_tag="VM692_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ52437.1"
                     /translation="MSRRTTLATPVTAAFALVLTISASSQETTVYHGFTRLDPVTATA
                     TRNAFVVVQGDRIVSVGSGNPPRRDGSHSVDMSGRYALPGFFDTHAHITIGPLKVELK
                     DGAPAFRFEVIPDLHRHNALLALAYGVTTIRNPAGDAEANAHYGAMIASGEWLGPQAV
                     HAGDLMNPGFPHYPRNNQEWDIELQRQRDLGMTYAKLYSGLSEQELARGIETAHAHGL
                     KTIAHLDGVSWTRAMELGIDELTHALPTSADLLVEPNRTAYLESRKAPDAKYFYRWFE
                     LVDYDSEPFQTMLRMLVEKKIHVDLTLIANEIVYFYDRIDKELTYPLELAYAGAGFQQ
                     NWRQAMTASHVGWTEDDYRRAHTVMPKVLELAKRFYDAGVRLSMGTDGTGGGPSFVRE
                     LALHVEAGIPPWEVLRLATSGAAERLGMADRRGALAPGFEADIVFLSDDPLESIDNVR
                     SVDVVVQDGRAYRAANLLELITGPR"
     gene            complement(2797..3309)
                     /locus_tag="VM692_09465"
     CDS             complement(2797..3309)
                     /locus_tag="VM692_09465"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52438.1"
                     /translation="MRTATIRSGRGRALRHASLVLGSVIGILVLAAWTARAQAHHSFS
                     VFDMNTTKEIDGTIVEFQWTNPHTWTWIDVKNADGSTTRWGLEGMSPNFLGRRGWTKS
                     TFEPGAKVHAWLWPLKSGEPGGTLQKATLPDGSEVVNFGGGGGGGGGGGGGGGGGGGG
                     GGRAGGGAPQ"
     gene            complement(3306..4052)
                     /locus_tag="VM692_09470"
     CDS             complement(3306..4052)
                     /locus_tag="VM692_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52439.1"
                     /translation="MLRHAWLIAVIAAAPLTAQKSGPQGETWDSIAKLPDFGGLWEVT
                     FGGGPRGAGEPPALTPPYAAKLAEYQAASRAGEIEDGPAANCVPNGMPSIMTQPYPIE
                     ILFTPGKVTILIEAYAQWRQIFTDGRAHPEDPDLTFNGHSIGHWEGDTLVVDTVGFTT
                     DTFMGGGGMRHSDKMHIVERFKLAQPDRLEVQTTVDDPEALTKPFTRTAAYGRHRDWT
                     LAEYVCQQNNRNFTTDDGKSGIDLTFKERE"
     gene            4263..4943
                     /locus_tag="VM692_09475"
     CDS             4263..4943
                     /locus_tag="VM692_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52440.1"
                     /translation="MSMLHPAESPGPRSWLEGALARFAAGNADLVERTRTLLDAVIAE
                     PQRHARMINTLSLLEHMGSHKIMSTQHSAAIDQATLKHVAEEAQHAYFMKRQAEKTAE
                     RELEYVARDLLAPSSARMYFQRLEGATVRTLGPRRSSRAAYLYMSMIVEFRALWFYGL
                     YDQALKRARHAMSLKRVLGEEQAHLGDMAERLEAGHELDDARTAAVLAAERVLYARLL
                     AAIERAVA"
     gene            5098..5541
                     /locus_tag="VM692_09480"
     CDS             5098..5541
                     /locus_tag="VM692_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HUQ52441.1"
                     /translation="MALVRYEPWGIGQLQSEINRLFSSFGDGESSGVNATWVPPVDIY
                     EHENRFQLYMDLPGVDPASVDITLEAGVLTVTGERMHFGGPQDVNDRGDLRRNERGLG
                     RFYRRFVLPDSVDSERVEATNRHGVLELTIPKQAKALPRRIKVAA"
     gene            5698..>5939
                     /locus_tag="VM692_09485"
     CDS             5698..>5939
                     /locus_tag="VM692_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52442.1"
                     /translation="MGRFVRQAILIAAALAAAAAFVRATHAQEPTGRTTLIELTPHLA
                     VNDAASLIEMQNYADAIDILDTFNANQDQAVPEAYYL"
BASE COUNT         1070 a         2022 c         1870 g          977 t
ORIGIN      
        1 tcgaactccc gaccctcagg ttcgaagcct gatgctctat ccaactgagc tacgggcgcg
       61 tgagggcgat tgtaaaggag cgcgtcggcg ccgtcagtcg gtccggagct ggcgttcgag
      121 ctcgcgcagg cgctcgagct cggcccgccg ccggcgatgg aaccagtaga tcagcgcaag
      181 gccggccggt atgccgatcc agccgacgac gaccatgaag ctcgacgtca atatgcgcgc
      241 gacgccgccc ggcaactctg cggcgagcgc aagcacgacg aacacgagct gcgcgacgta
      301 gagacacaaa gcgaagatcg ccgcgcgcgc attcgctttg cgacggcgga tcgacacggc
      361 gacgaacgcg gccgtcgtgt cggcgagcgg ccgccaggtc ccgcgcgcga gccacagggc
      421 gccgatccac gtcacgacga tgaagatcca ggtttcgacg gcgaacagga cgtcgagagg
      481 ctgctgcgtg cgcagcgcgc gcagcgtcat gccgccaccg acgccgagcg tgacgagaat
      541 cggcccgatg agactccact tgagccaccg cgtctcgcgc agcacgcgct gccgtaactg
      601 cacgaccgca tcggccggcg gcgcggctgc cccttgccat tgccgtcgcc aactctccag
      661 ttctgcgtct tggctcatgt cccctcctcg agaagctctt tgagtctttg ccgcgcgcgg
      721 ttcaggcgca cgccgacgtt gctttcactg atgccgacga ccgccgcgat ctcgccgtaa
      781 tccatgcctt cgagaaacag cacgagcacc tcgcggtgaa ttgcaggcag gccgcgaatc
      841 gcctgaacaa agcggcggcg ctgctgttcc tcggccagca gtgtttccgc ggagacgttt
      901 ggatcctcga cgtcgatctg cgcttcgtcg agcgacaccg tggggcgccg tttcgacagg
      961 tgcgtcatac agcggttgtg cgcgatgcga aacagaaacg tgcgctccga gcaatcgccc
     1021 ctcaagctcg gcaacgcacg ccacaccgcg atggcgatct cttgtagcaa gtcgtcgcga
     1081 tcgctgcggt tgcccgcgta gctcgccgcg agccgcgtca gcggcgcgtg attcgcctcg
     1141 agcaagcgcc gaaagcgctc gtcaagcgag tcaccggctc gctcggcaac ctgccttccg
     1201 cctgccgtcg tggtcgtcac gcgctaatag tcccggccgc gcgcgaatta ttacagccct
     1261 tccgctaagc agtttgtaag aaaacccgcg gcgcctagga cagtagtagt ccgtcaacag
     1321 gaatcacggt aggagcgcaa ggtatgagcc gcagaacgac gctcgccacg cctgtgacgg
     1381 cagcgttcgc cctggtgctt accatcagcg cctcgagcca ggaaactacc gtctatcacg
     1441 gcttcacgcg gctcgacccc gtcacggcga cggcgacgcg gaatgctttc gtcgtcgtgc
     1501 aaggcgatcg catcgtgagc gtcggcagcg gcaatccgcc ccgccgtgac ggctcacact
     1561 ccgtcgacat gtcgggtcgc tacgccctgc ccggcttctt cgacacgcac gctcacatca
     1621 cgatcggccc gttgaaggtc gagctcaaag acggcgcgcc ggcgtttcgc ttcgaggtca
     1681 tccctgatct ccaccgccac aacgcgctgc tcgcgttggc ctacggtgtc acgacgatcc
     1741 gcaacccggc cggcgacgcc gaagccaacg cgcactacgg cgcgatgatc gcgagcggcg
     1801 agtggctggg cccgcaggcc gtgcacgccg gcgatctcat gaaccccggt ttcccccact
     1861 acccgcgcaa caaccaggag tgggatatcg agctgcaacg gcagcgcgac cttggcatga
     1921 cctacgccaa gctttactcg gggttgagcg agcaggagct cgcgcgcggc atcgagacgg
     1981 cgcacgcgca cggactgaaa acgatcgcgc atctcgacgg cgtgagctgg acacgcgcca
     2041 tggagctcgg gatcgacgag ctcacgcacg cgctgccgac gagcgcggat ctattggtcg
     2101 agccgaatcg cacggcgtat ctcgagagcc gcaaagcgcc cgacgcaaag tacttctatc
     2161 gctggttcga gctcgtcgac tacgacagcg agccgttcca gaccatgctg cgcatgctcg
     2221 tcgagaagaa gattcacgtc gacttgacgt tgatcgcgaa cgagatcgtg tatttctacg
     2281 accgaatcga caaagagctc acgtatccgc tcgagctcgc gtatgcgggc gccgggttcc
     2341 aacagaactg gcggcaagcc atgaccgcct cgcacgtcgg ctggacggaa gacgactacc
     2401 ggcgcgcaca caccgtgatg ccgaaggtcc tcgagctcgc gaagcgcttc tacgatgccg
     2461 gcgtgcggtt atcgatgggc acggacggaa cgggcggcgg gccgagcttc gtgcgcgagc
     2521 tcgcgttgca cgtcgaggct gggatcccgc cgtgggaagt gctgcggctc gcaacgagcg
     2581 gcgcggccga gcggctcggc atggcggatc gcaggggagc gctcgcgccc ggattcgagg
     2641 ccgacatcgt gttcttgagc gacgatccgc tcgaaagcat agacaacgtc cgcagcgtcg
     2701 acgtcgtcgt gcaagacggc cgcgcctatc gagccgcgaa ccttctcgag ttgatcacgg
     2761 gaccgcgcta ggtcggcaga aacgaggagc tgatctttac tgcggcgcgc cgccgccggc
     2821 tcgtccgcca ccaccgccgc caccgccacc gccaccgcca ccgccgccgc cgccgccgcc
     2881 gccgccgccg aagttaacga cctcgctgcc gtccggcaac gtggccttct gcagcgtccc
     2941 gccgggctcg ccgctcttca gaggccagag ccaggcgtga acttttgcgc cgggctcgaa
     3001 cgtgctcttg gtccacccgc gtcggcccaa gaaattcggg ctcatgcctt cgaggcccca
     3061 gcgcgtcgtc gagccgtcgg cgttcttgac gtcgatccac gtccacgtgt gcgggttcgt
     3121 ccactgaaac tcgacgatcg tgccgtcgat ctccttcgtc gtgttcatgt cgaacacgct
     3181 gaacgagtga tgcgcctgcg cgcgcgctgt ccaagcggcg agcacgagaa tgccgatgac
     3241 gctgccgagc accaagctcg cgtgacgcag cgcgcgaccg cggccgctac gaatcgtcgc
     3301 cgttctcatt ctcgctcctt gaacgtcagg tcgatgccgg acttgccgtc gtcagtcgtg
     3361 aagttgcggt tgttctgctg gcacacgtac tcggcgagcg tccaatcgcg atgacggccg
     3421 tacgcagcgg tgcgcgtgaa cggcttcgtc aacgcttcgg gatcgtcgac ggtcgtttgc
     3481 acctcgagcc gatcgggctg cgcgagcttg aagcgctcga cgatgtgcat cttgtcgctg
     3541 tggcgcatgc cgccgccgcc catgaacgtg tccgtcgtga agccgacggt gtcgacgacg
     3601 agcgtgtcgc cttcccaatg accgatcgag tgaccgttga acgtgaggtc gggatcttcg
     3661 ggatgtgcgc ggccgtccgt gaagatctgc cgccactgcg cgtacgcctc gatcaggatc
     3721 gtaaccttcc ccggcgtgaa taagatttcg atcgggtacg gctgcgtcat gatcgacggc
     3781 atgccgttcg gcacgcagtt cgccgcaggc ccgtcctcga tttcgccggc tctgctcgcg
     3841 gcctgatact ccgcaagctt cgccgcgtac ggcggcgtca gcgccggcgg ctcgccggcc
     3901 ccgcgcggcc cgccgccgaa cgtaacttcc cacaagccgc cgaagtccgg cagctttgcg
     3961 atcgagtccc aggtttcgcc ttgcggcccg gatttctgcg ccgtgagcgg cgcggcggca
     4021 atgacagcta tcaaccaagc gtggcgcagc atgcgttccc ccaaagccct gcgcgtacac
     4081 tagcagccca cggcgcaggc agtctggaga attttcagtc tcactggcct cgcacctcgg
     4141 gtccaaggat tcaggcggat gccgtcgacg tcgctgccta tatcgcgacg ctgcgcgact
     4201 gacgaggcgc cgctgtcggc gccggttcgc gtacactggg ctccccgacg aaacctctgt
     4261 tcgtgagcat gctgcatccc gcggaatctc cggggccgcg ctcgtggctc gaaggcgcgc
     4321 ttgcgcgctt tgccgccggc aacgccgacc tcgtcgagcg aacacgcacg ctgctcgacg
     4381 ccgttatcgc cgagccgcag cggcacgcgc gcatgatcaa cacgctgtcg ctgctcgagc
     4441 acatgggcag ccacaagatc atgtcgacgc agcattcggc ggcgatcgat caggcgacgc
     4501 tgaaacacgt cgccgaagaa gcgcagcacg cgtatttcat gaagcggcag gccgagaaga
     4561 cggccgagcg cgagctcgaa tacgtcgcaa gggatttgtt ggcgccctct tccgcgcgca
     4621 tgtatttcca gcggctcgaa ggcgcgaccg tgcgaacgct tgggccgcgc cgcagctcgc
     4681 gcgccgccta tctctacatg tcgatgatcg tggagttccg agcgctctgg ttctacggcc
     4741 tctacgacca ggcactgaag cgcgcgcgcc atgcgatgtc tttaaagcgc gtgctcggcg
     4801 aggagcaagc gcatctgggc gacatggccg agcggctcga agccggccac gagctcgacg
     4861 acgcacgaac ggcggcggtg cttgcggccg aacgcgtcct ctacgcgcgc ctgctcgcgg
     4921 cgatcgagcg cgccgtcgcc tgagacatta cgtaaccgta atgcgcgtgc gggcgagacc
     4981 gcgccttgaa aaaaagcccc tccaaccata caaaaaagcg cgttccggcg gttctcgttc
     5041 gactcgaggc tgggccgcgc ggggcaacaa actgaagacc aaggaggatg atgaactatg
     5101 gcacttgtca gatatgaacc gtggggtatc gggcagctgc agagcgaaat caaccgcctg
     5161 ttcagctcgt tcggcgatgg cgaaagcagc ggcgtcaatg cgacgtgggt tccgcccgtc
     5221 gacatctatg agcacgagaa ccgattccag ctgtacatgg atctccccgg cgtcgacccg
     5281 gcgagcgtcg acatcacgct cgaggccggc gtgctgaccg tcacgggcga gcgcatgcac
     5341 ttcggcggtc cgcaggacgt caatgatcgg ggcgacctgc gccgcaatga gcgcggcctc
     5401 ggccgcttct atcgccggtt cgtgctgccg gacagcgtgg actcggagcg cgtcgaagcg
     5461 accaatcggc acggcgtgct cgagctcacg atcccgaagc aagcgaaggc actgccgcgc
     5521 cgcatcaaag tcgcggccta agcgcaaaag cgctatccgt ttgcgcacgg acggttcggg
     5581 gggcctcagc cccccgaacc ccttcttcgc cgcaaccgcg taccgaacgt tacgttcaga
     5641 gccttccgcg cggccgcagc atcggcgtca agtacactaa gcgcgcaaag acacgccatg
     5701 ggcaggttcg tccgacaagc aattctcatt gcagccgcgc tcgcggcagc ggcggcgttc
     5761 gtgcgcgcga cgcacgccca agagcccact ggtcgaacga cgttgatcga gctgacgccg
     5821 cacctcgccg tcaacgacgc cgcgtcgcta atcgagatgc agaactacgc cgacgccatc
     5881 gacattctcg atactttcaa cgccaaccaa gaccaagctg tccccgaggc ctactacct
//
LOCUS       DATLEE010000442         3063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5710929, whole genome shotgun sequence.
ACCESSION   DATLEE010000442 DATLEE010000000
VERSION     DATLEE010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5710929"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VM692_09490"
     CDS             <1..293
                     /locus_tag="VM692_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52443.1"
                     /translation="SSIRARHALIVGMRQVDLVTLATSPTLAPPTSGRAVYGISVGGD
                     IRLFTTFAEFSTELALTIGGGRPALALTASGSYDAATTTLHATHIAVHFAGN"
     gene            complement(349..810)
                     /locus_tag="VM692_09495"
     CDS             complement(349..810)
                     /locus_tag="VM692_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018000685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HUQ52444.1"
                     /translation="MHGLYYDEFEVGRVFKHAIRRTLTETDNILFSTMTHNPAPLHID
                     YEYMRTQSEFRKPLMNSFFTLGLIVGISINDTTLGTTVANLGLESARFPAPLFVGDTV
                     HVETEVVAMRVSESRPRNGILTLEHRGYNQSDVLVAICRRNVLMLRSPNPP"
     gene            complement(820..1635)
                     /gene="proC"
                     /locus_tag="VM692_09500"
     CDS             complement(820..1635)
                     /gene="proC"
                     /locus_tag="VM692_09500"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HUQ52445.1"
                     /translation="MSATETILVIGFGNMGQALVRGWLACGRAASTIVVVEAMQAARS
                     VAKSLGIATQQDLEVVTGSADVIVLAVKPNQLANVLPRCTACAASNAVVLSIAAGKTI
                     AEIGKALGRDAAIVRAMPNTPAAIGKGMTTMVANSAVTPAQRTLCTELLAAVGAVAWL
                     DDEAGMDAVTAISGSGPAYVFLLIECLEQAAIDLGLGAALARQLAVATVEGAGAYAAA
                     TAEAPAELRRHVTSPNGTTQAALDVLMGEPGMRELFIRAARAAAKRGRELASA"
     gene            1772..2929
                     /locus_tag="VM692_09505"
     CDS             1772..2929
                     /locus_tag="VM692_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HUQ52446.1"
                     /translation="MFIAGVGQTPITKDPQSRGRYLGAAAVKAALADSGLEPNRIGAL
                     YVGNMLSGILAQQQQLGGLIADYTGLTGIEAVTIEAACASGGAAMRVAYQAVAGGIHD
                     AVAVCGVERMTHVERDEVTRALATAADWELEGVCGESFLSLNAKLMRAYMDKYAVTAE
                     RFAPFAITAHRNALTNPNALLHKPLDLETYLESRIVTDPIRLFDVSPVCNGAGAVILA
                     APHVAGSLPGGSKVRIAGSALSTAPLALARRADPLELTAVGSSTRQAMQQAGIGHDDI
                     DLFEPHDAYTIMTALALEAAGFARAGAGLDFADCARIGLGGELPLATFGGLKARGHPV
                     GASGCYQIVEAYLQLTQRAGANQVEDAGVALVQNIGGTGATVVTHVLTREN"
BASE COUNT          498 a         1033 c         1007 g          525 t
ORIGIN      
        1 attcgagcat ccgtgctcgc cacgcgctga tcgtcggcat gaggcaggtc gacttggtga
       61 cgttggccac gtcgccgacg ctggcgccgc ctacgtcggg acgcgccgtc tacggcatct
      121 cggtcggcgg cgacattcgt ctgttcacga ccttcgcgga gttcagcacg gagctcgcct
      181 tgacgatcgg cggcgggcgt ccggcgctcg cgctgacggc gagcggcagt tacgacgcgg
      241 cgaccacgac gctgcatgcg acgcacatcg ccgtgcattt cgccggcaac tgaaacttct
      301 tcgacgttcg acgagccccg ttcgcggggc tcgtcttttt ttggagattc agggcggatt
      361 cggcgagcgc agcatcaaca cgttgcgccg acagattgcg acgaggacgt cgctctggtt
      421 gtagccgcga tgttcgagcg tgaggatgcc gttgcgcggc cgtgactcgc tgacgcgcat
      481 ggcgacgacc tccgtctcga cgtggaccgt gtcgccgacg aacagcggcg ccggaaaacg
      541 cgcgctctcg aggcccaagt tcgcgacggt cgtcccgagc gtcgtgtcgt ttatcgagat
      601 gccgacgatg aggcccagcg tgaaaaagct gttcatcagc ggcttgcgga actccgactg
      661 cgtgcgcatg tactcgtaat cgatgtgcag cggcgcgggg ttgtgcgtca tcgtgctgaa
      721 caggatgttg tccgtctcgg tgagcgtgcg gcggatggcg tgcttgaaga cgcgcccgac
      781 ctcgaactcg tcgtagtaga gcccatgcat gtaccgctcc tatgcgctcg cgagctcgcg
      841 gccgcgtttc gccgctgcgc gcgcggcgcg aatgaacagc tcgcgcatgc cgggctcgcc
      901 catcagcacg tcgagcgcgg cttgcgtcgt gccgttcggg ctcgtcacat ggcggcgcag
      961 ctccgcgggc gcctccgccg ttgcggccgc gtatgcgccc gcgccttcga ccgtggcgac
     1021 cgcgagctgc ctcgccaaag ccgctccgag cccgagatcg atcgccgcct gctcgaggca
     1081 ttcgatcaac aggaacacgt aggcagggcc gctgcccgaa atcgccgtca cggcgtccat
     1141 cccggcctcg tcgtcgagcc aagccacagc gccgacggcg gcgagcaact ccgtgcagag
     1201 cgtgcgctgc gccggcgtca ccgcggagtt cgcgaccatc gtggtcattc ccttgccgat
     1261 ggcggccgga gtgttcggca tcgcacggac gattgccgcg tcgcgtccca gcgccttgcc
     1321 gatctccgcg atcgtcttgc ccgctgcgat cgagagcacg acggcattgc tggccgcgca
     1381 agcggtgcag cgcggcagca cattcgcgag ctgattcggt ttgacggcga gaacgatgac
     1441 atccgcgctt ccggtcacga cttccaaatc ttgctgcgtc gcgatgccga gcgacttcgc
     1501 gacggaacgc gccgcctgca ttgcctcgac gaccacgatc gtgctcgcgg cgcggccgca
     1561 ggcgagccag ccgcgcacga gtgcttggcc catgttgccg aacccgatca ccaaaatcgt
     1621 ttcagttgcg ctcaactcga ttcctccaag ccttgctctg catgatagcc gatgcctcga
     1681 ctcgcgcggc tcgcgtccgc cggttggcaa tcgatgcgac cgtttgccat gatgcgcgcc
     1741 ggtcaacggc tcggagcgcc agttgagaga cgtgttcatt gccggtgtgg gtcagacacc
     1801 gatcacgaag gatcctcaaa gtcgcggccg ttatctgggc gcggcagccg tcaaggcggc
     1861 gctggcggac tcgggcctgg aacccaaccg aatcggcgcg ctatacgtcg gcaacatgct
     1921 ctccggtatc ctggcgcaac agcagcagct cggcggcctg atcgccgact acacggggct
     1981 tacgggcatc gaagccgtta cgatcgaagc ggcgtgcgcg tccggcggcg ccgcaatgcg
     2041 cgttgcctat caagccgtcg cgggcggcat tcacgacgcc gttgccgtct gcggagtcga
     2101 acgcatgact cacgtcgagc gagatgaggt gacgcgcgcg ctcgcgacgg ccgccgactg
     2161 ggagctcgag ggcgtctgcg gcgagtcgtt cttgtcgttg aacgccaagc tgatgcgcgc
     2221 gtacatggac aaatatgccg tcacggcaga gcgcttcgcg ccgtttgcga tcacggcaca
     2281 tcggaacgcg ctcacgaatc ccaacgcact gctgcacaag ccgctcgatc tcgagacgta
     2341 tctcgagtcg cgcatcgtca cggatccgat ccgcttgttc gatgtctcgc cggtctgcaa
     2401 tggcgccggc gccgtcattc tcgccgcgcc gcacgtcgcc gggagcctgc ccggcgggtc
     2461 gaaggtccgc atcgcgggct cggcactctc gaccgcgccg ctcgcgctcg cgcggcgcgc
     2521 ggatccgctc gagctcacgg ctgtcggcag ctcgacgcgg caagccatgc agcaagccgg
     2581 catcggccat gacgacatcg acctattcga gccgcacgac gcgtacacga tcatgaccgc
     2641 gttggcgctc gaagccgcgg gcttcgcacg cgccggcgca gggctcgact ttgccgactg
     2701 cgcgcgcatc gggctaggcg gcgagttgcc gctcgcgacg ttcggcgggc tcaaagcgcg
     2761 cggtcacccc gtcggcgcca gcggctgcta tcagatcgtc gaggcgtacc tgcaactcac
     2821 gcagcgcgcc ggcgcaaatc aggtcgagga tgcaggcgtc gcgctcgtgc agaacatcgg
     2881 cggcacgggt gcgacggtcg tcacccacgt cctgacgcgc gagaactaag cccgccagaa
     2941 aatgctagcc tatgcggctt atcgcacagc ggcttgagac gaagaggaat ccgccatggc
     3001 agacgagctc accgatgccg atatcgcaat ggacagcacc ggtctctacc acgaggagac
     3061 ttt
//
LOCUS       DATLEE010000443         5265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4199702, whole genome shotgun sequence.
ACCESSION   DATLEE010000443 DATLEE010000000
VERSION     DATLEE010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4199702"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VM692_09510"
     CDS             complement(<1..294)
                     /locus_tag="VM692_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HUQ52447.1"
                     /translation="MNAYKTEFLELALELGVLKFGEFTLKSGRISPYFFNAGLFNSGY
                     AAAKLGRCYASAIAALDFKFDMLFGPAYKGIPLVVLSAASLASHLDEDYPFAFN"
     gene            358..1134
                     /locus_tag="VM692_09515"
     CDS             358..1134
                     /locus_tag="VM692_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HUQ52448.1"
                     /translation="MRVVTFNANGLRSAQRKGFFSWFARQRADVLCVQELKARDEQIA
                     DDAFHARGFHRACVHAEKAGYSGVAIYSKAEPVEVVRGIGEPEFDREGRYVEARFGGA
                     AVVSAYFPSGSSGPARQEAKLRFLDAFDRRLVELETLGLPFVFCGDFNMAHRPIDLKN
                     WRSLQDYPGFTPVERAWLDSLFTGRGFVDAFRAVNQQPHQYTWWSNRGQAWANNTGWR
                     IDYQVVSAALGATIRSAAIYKRRRFSDHAPLTIDYDWQLT"
     gene            1131..2447
                     /locus_tag="VM692_09520"
     CDS             1131..2447
                     /locus_tag="VM692_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ52449.1"
                     /translation="MTQSGWRLSLPESVRQYTEAAPLAALFLGISSGAPYAMIGATLT
                     TRLAQDGITKSSITAFSLAFLVYNLKWLWAWVVDGVRLPLLGRLGQRVSWLVFAGFLS
                     IAAITNLGLVDPQSSLSATAVAAILVAVAGATFDIVIDAYRIESLEPQQLGVGSGMSQ
                     YGWRIGSVLAASLALLIAARAGWQLAYIACAVFIVPAMAVGLILGEPLRRQSVAAWVG
                     FAAIAQSVVGPFLEFFRRHGALLVLLFVLVHKVGDTLGQLTLRLLLNDLSYTNDEIAF
                     YDVGVGFWTYLAGIFVGASLYARLGVARSVFLSLWLMAISNLSFAALAAVGKSNLGLA
                     GAIGFENFASGLGGVVVVAYFSALCDVRYTAAQFALLTAAMSVVGRIATGAAAGALVE
                     RFGYTTFYLLTTAAAVPGIVLFWLMMRRGLASPPPAQPAAAVEPVH"
     gene            complement(2461..3165)
                     /gene="trmB"
                     /locus_tag="VM692_09525"
     CDS             complement(2461..3165)
                     /gene="trmB"
                     /locus_tag="VM692_09525"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018868174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HUQ52450.1"
                     /translation="METPGEAPAHLRKVRSFVRRPGRATAAQRRAFAELLPRFDANAA
                     EGPLDLVRVFGREAPRVLDIGFGDGEALVTNALNNPTVDYLGIEVHEPGIGHLLLLLE
                     QAASENVRVIARDAAEVVPQLLPTASFDVVDLFFPDPWPKKRHHKRRLVQPPFVAELA
                     RVLKPGGFLHVATDWADYARHTREVLMADARFAEVAGEFLASEPLAARPPTKFERRGM
                     RLGHEVSDLYYRALPR"
     gene            complement(3257..3330)
                     /locus_tag="VM692_09530"
     tRNA            complement(3257..3330)
                     /locus_tag="VM692_09530"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3296..3298),aa:Gly,seq:ccc)
     gene            3585..4790
                     /locus_tag="VM692_09535"
     CDS             3585..4790
                     /locus_tag="VM692_09535"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HUQ52451.1"
                     /translation="MADNVNRLLIVDGDGAVADFVGKIARKVGYAVASAKSGATFVQL
                     LESFQPSLIVMELHLPDTDGVELLRSLAARACAANVVLISAVDERVLSAAHELGVSHG
                     LAMCGTLEKPLPVGELTSKLSAMLHQSPELDADDLRRGIGSNEFVPYYQPKASLVQSG
                     WIIDGVEALARWEHPRLGLVMPNEFIPLAEQTGLIGELTSVMLNAALKQLRAWQEQGV
                     RLTCAVNLPPSLVTDLSFPDRVAALLAENGVDGSQLVLEITETATIQNPTATMAILTR
                     LRVKRVGLSLDDFGTGYSSLTQLYQMPFNEMKIDKSLVMNVPHSREANTMVGSLIELG
                     HNLGLKICAEGVENRAALDMLAIMGCDRCQGYFISRAVPASEIVSFATQWNRDNPASS
                     FTHLPRAVV"
     gene            4810..>5265
                     /locus_tag="VM692_09540"
     CDS             4810..>5265
                     /locus_tag="VM692_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUQ52452.1"
                     /translation="MSDTPPNDNRLMVMDDDVDVGAFFGQVGEDLGFAVRVISDPTLF
                     AQHVLEFSPTVLLLDLQMPGRDGIELLRELSTLERDPKVLIASGLDSRVLTSAAELGV
                     SMGVEIAGAFCKPIPLDELEVLLTRLKSHTKRITANQLREAVHQGQLVVH"
BASE COUNT          850 a         1693 c         1728 g          994 t
ORIGIN      
        1 gttgaaagcg aatgggtagt cttcgtcgag gtggctcgcc agagacgcgg ccgacaacac
       61 gacgagcgga attcctttgt acgcagggcc gaatagcatg tcgaacttga agtcgagcgc
      121 cgcgatggcc gacgcataac aacggccgag cttcgctgcg gcgtaaccgc tgttgaacaa
      181 accggcgttg aagaagtagg ggctgatgcg gccggacttc agcgtgaact cgccgaactt
      241 caggacgccg agctcgagcg cgagctccag gaattcggtt ttgtaagcgt tcatcggtgg
      301 ccggtcgctg aggcgtgcgt atcatatcgc ttccgttttc caaccggccg gtttttcttg
      361 cgagtcgtca ccttcaacgc gaacgggttg cgcagcgcgc agcgcaaggg tttcttctcg
      421 tggttcgcac ggcagcgcgc cgacgtcctc tgcgttcagg agctcaaggc gcgcgacgag
      481 cagatcgccg acgatgcgtt tcacgcgcgc ggctttcatc gcgcctgcgt gcacgcagag
      541 aaggcgggtt acagcggtgt tgcgatctac tcgaaggcgg agcccgtcga ggtcgttcgc
      601 ggcatcggcg agcccgagtt cgatcgcgag ggacgctacg tcgaagcgcg cttcggcggc
      661 gccgcagtcg tcagcgcgta ttttccgtcc ggctcgtcag ggcccgcgag gcaagaagcg
      721 aagctgcggt tcctggacgc gttcgatcgg cgactcgtcg agctcgagac gctcggcctg
      781 ccgttcgtgt tctgcggcga cttcaacatg gcgcatcggc ccatcgatct gaagaactgg
      841 cgctcgttgc aggactatcc cggcttcacg cccgtggagc gcgcgtggct cgactcgctg
      901 ttcacggggc gcggcttcgt cgatgccttt cgcgccgtga atcagcagcc gcatcaatac
      961 acgtggtggt cgaatcgcgg ccaagcgtgg gccaacaaca ccggctggcg aatcgactac
     1021 caagtcgtga gcgccgcgct cggcgcgacg atccgtagcg cggcgattta caagcggcgg
     1081 cggttctcgg accacgctcc gctgacaatc gattacgatt ggcagctgac gtgacgcaat
     1141 ccggctggcg actttccctt cccgaatcgg ttcggcaata caccgaggct gcgccgctcg
     1201 ccgcgttgtt cctcggcata tcgtcgggcg cgccgtacgc gatgattggg gcaacgctga
     1261 cgacgcgtct cgcccaagac ggcatcacga agagctcgat cactgcgttc tcgcttgcgt
     1321 tcttggtcta caacctgaag tggctttggg cgtgggttgt ggacggcgtt cgcctgccgc
     1381 tcctcggccg acttgggcag cgcgtgtcgt ggctggtgtt cgccggcttt ctgtcgatcg
     1441 cggctatcac gaacctcgga ctcgtcgatc cgcagagcag cctttccgcg acggccgtgg
     1501 ccgcaattct tgtggcggtt gcgggcgcga cgttcgacat cgtcatcgac gcgtaccgaa
     1561 tcgagtcgct cgagccgcag cagcttggcg tcgggtctgg catgtcgcag tacggatggc
     1621 gcatcggctc cgtgcttgcg gcttcactgg cgctcttgat cgccgcgcgc gccggttggc
     1681 aactcgccta catcgcgtgc gccgtcttca tcgtgcccgc aatggccgtt ggcctgatac
     1741 tgggcgagcc gctgcgacgc cagtcagtcg ccgcttgggt aggatttgcg gccattgccc
     1801 agtcggtcgt cggcccgttt ctcgagttct tccgtcgcca cggcgcgctg ctcgtactgc
     1861 tgttcgtgct cgtccacaag gtcggcgaca cgctcgggca actgacgttg cggctcttgc
     1921 tgaacgacct ctcctacacg aacgacgaga tcgcgttcta cgacgtcgga gtcggcttct
     1981 ggacgtacct cgcggggatt ttcgtcggtg catcgttgta cgcgcgcctc ggggtcgcgc
     2041 gctccgtgtt cctgagcctt tggctcatgg cgatctcgaa cctgagcttc gcggctctcg
     2101 ctgcagtcgg gaagtcgaat ctcgggttgg ccggagcaat cggtttcgaa aactttgcca
     2161 gcggcctcgg cggcgtcgtc gtcgtcgcct atttctcggc actctgcgac gtgcgttaca
     2221 cggctgcgca gttcgcgctg ctgacggcgg cgatgagtgt cgtcgggaga atcgctacgg
     2281 gtgcggcggc cggcgcgctc gtcgagcgct tcggatacac gaccttctat ctattgacga
     2341 cggcggcggc agttccgggc atcgtgctct tctggctgat gatgcgccgc ggcttggcga
     2401 gcccgcctcc ggctcagccc gcggcggcgg tcgagcccgt gcactgacgc gcggcagcgc
     2461 tcaacgcggc agcgcgcgat agtagaggtc gctgacttcg tgacccagcc tcatgccgcg
     2521 gcgttcgaat ttggtcggcg ggcgcgcggc gagcggctca gacgcgagaa actcacccgc
     2581 cacttctgcg aaacgcgcat cggccatgag tacctcgcgc gtgtgccgcg cgtagtcggc
     2641 ccagtccgta gcgacgtgca agaagccgcc cggcttcagc acgcgcgcaa gctcggcgac
     2701 gaacggcggc tgcacgagcc ggcgcttgtg atgacgtttc ttgggccacg gatccggaaa
     2761 gaaaaggtcg acgacatcga agctcgccgt cggcagcagc tgcggcacga cctcggccgc
     2821 gtcgcgcgcg atgacgcgca cgttctcgct tgccgcctgc tcgagcagca gcagcaagtg
     2881 gccgatgccg ggctcgtgca cctcgatacc gagatagtcg acggtcggat tgttcaacgc
     2941 attcgtgacg agcgcctcgc cgtcgccgaa gccgatgtcg agcacgcgcg gcgcttcgcg
     3001 cccaaacacg cggactagat cgagcggtcc ttcagctgcg tttgcgtcga atcgcggcag
     3061 cagctccgca aacgctcgcc gttgcgcagc cgtcgcgcgg ccgggccggc gcacgaagct
     3121 gcgaactttg cgcaggtggg caggcgcctc gccgggcgtt tccatggcat tcctcgctgg
     3181 tcgaaccgcg gagccgcagg cagacgttgg cgtcgatact acttcggttc ggccaggtca
     3241 gctttaacgt gaaaagtgga gcgggcgatg ggaatcgaac ccacggctct agcttgggaa
     3301 gctagggtat taccattata cgacgcccgc cccgatggcc tcggaatgct accaccggct
     3361 taggcttggc ctctagcgcc ggtcggtcag cggtcggccg gcgccctccg gcggacctgg
     3421 gtcactggcg ctcccgcgaa gctagacggc cgtcacacaa ttcggcgccg ccccgttcgt
     3481 ccggcatcag aaaaacctat tcttggccga cttagcaacg aacgtggaca cggcgtgctc
     3541 gccgggcctg cgccttcaag ttttcgggac tcgagctccg ctcaatggct gacaacgtca
     3601 ataggctgct gatcgtcgac ggggatggtg ccgtcgccga cttcgtcggc aagatcgcac
     3661 gcaaagtcgg ctatgccgta gcctccgcca agtcgggcgc gacgttcgtc caattgctcg
     3721 agtcgtttca accgtcattg atcgtcatgg agctgcatct gcccgacacc gacggcgtcg
     3781 agcttctgcg ctcgctggcc gcgcgcgcct gcgcggccaa cgtcgtgctc atcagcgccg
     3841 tcgacgagcg cgtgctctca gcggcgcacg agctcggcgt cagccatggg ctcgcgatgt
     3901 gcggcacgct cgagaagccc ctgccggtcg gcgagctcac gtcgaagtta tcggcgatgc
     3961 tgcatcagag ccccgagctc gatgccgacg acctgcgccg cggcatcggg agcaacgagt
     4021 tcgttccgta ctaccagccg aaggcctcgc tcgtgcagag cggctggatc atcgacggtg
     4081 tcgaagcgct cgcgcgctgg gagcacccgc gcttggggct cgtgatgcca aacgagttca
     4141 ttccgctggc cgagcagact gggctcatcg gcgagctgac gagcgtgatg ttgaacgccg
     4201 cgctgaagca gctgcgcgcg tggcaggagc agggcgtgcg gctcacgtgt gccgtgaatt
     4261 tgccgccgag cctcgtgacg gacttgagct tccccgatcg cgtcgcggca ctgctcgccg
     4321 agaacggcgt agacggcagt cagctcgtgc tcgagatcac cgagacggcg acgatccaga
     4381 acccgacggc cacgatggcg attctcacgc gcttgcgcgt caagcgcgtc ggcctctcgc
     4441 tcgacgactt cggcacgggt tactcgtcgc tgacgcagct ctatcagatg ccgttcaacg
     4501 agatgaagat cgacaagagt ctcgtcatga acgtgccgca cagccgcgaa gccaacacga
     4561 tggtcggctc gctgatcgag ctcggccaca acctggggct caagatctgc gccgaaggcg
     4621 tcgagaatcg cgccgcgctc gacatgctcg cgatcatggg ctgcgaccgc tgccagggct
     4681 acttcatcag ccgagcggta ccggcgtccg aaatcgtgag cttcgcgacg caatggaacc
     4741 gcgataaccc ggcttcctcg ttcacgcatc tgccgcgcgc ggtcgtgtga caactgctgg
     4801 agcacgaact tgagcgacac acccccgaac gacaatcgcc tcatggtcat ggacgacgac
     4861 gtcgacgtcg gcgcgttctt cggccaagtc ggcgaggact taggcttcgc ggtccgagtc
     4921 atctccgatc cgactctgtt tgcgcagcac gtgctggaat tctcgccgac cgtgctgctg
     4981 ctcgacttgc agatgccggg acgcgacggc atcgagctgt tgcgcgagct gtcgacgctc
     5041 gagcgcgatc cgaaggtgct gatcgcgagc gggctcgata gccgcgtctt gacgtcggcg
     5101 gccgagctcg gcgtgtcgat gggcgtcgag attgcgggcg cgttctgcaa gccaattccg
     5161 ctcgatgagc tcgaggtgct gctgacgcgc ttgaagtcgc acaccaagcg catcacggcc
     5221 aatcagctgc gcgaggccgt gcatcaaggc cagctcgtcg tccac
//
LOCUS       DATLEE010000444         6190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2732802, whole genome shotgun sequence.
ACCESSION   DATLEE010000444 DATLEE010000000
VERSION     DATLEE010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2732802"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VM692_09545"
     CDS             <1..922
                     /locus_tag="VM692_09545"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HUQ52453.1"
                     /translation="AVVAAAARAAAQPPAPGFADALLGRWDLTVEAADGAYPSWLEVR
                     LRTEGSLMGRFVGRVGSARYVDDVTFAAGRVAFSVPVQYESGIDEVRFEGVLHGERIE
                     GTARIDDGATARFTAVRAPALTRSTARELGAPTPLLNGRDLTGWAPRSQQHAGCWRMQ
                     GGVLAATPPCVDLVTTATFDDFRLHAELRYPRGSNSGVYLRGRYEVQIQDDAGKALDP
                     LRIGGLYGFVAPSADAALRAGEWQTLDVELVGRRVTVTLNGTTIIDAQEIPGITGGAL
                     DSDESAPGPIMLQGDHGAIEFRNVTIAPAR"
     gene            complement(933..1928)
                     /locus_tag="VM692_09550"
     CDS             complement(933..1928)
                     /locus_tag="VM692_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUQ52454.1"
                     /translation="MGSMTRCGASLAALLAAAAPAVASAQTLAEQLAWAYAITPGPAP
                     PATPDDGTLHSLPGSTGKFTRTQISNRMGPADWYPGDHPTMPPIVAKGREAAGVWACS
                     LCHYPNGKGRPENAGVAGLPKEYFIQQMYDFKNGARMSADARKPNTPIMAGFAVQMTD
                     EEIRAAADYFGSMKWTPWIQVVETDTVPKTRIAGGMHLKLEGAEAGTEPLGQRIVETP
                     VNAEHTELLRNPRSGFIAYVPKGSVKRGEELARFGNEGVTACTVCHGQDLNGLAMVPS
                     LRGRSPSYIARQLADMKEGKRHGAWTPLMEPVVASLGAEDILNLAAYLTSLEPLP"
     gene            complement(2027..3112)
                     /locus_tag="VM692_09555"
     CDS             complement(2027..3112)
                     /locus_tag="VM692_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52455.1"
                     /translation="MKHPFAIIVAAALLACAIAPSVATAEPYLAVESGLKCGNCHVNP
                     SGGGKRNAFGTLYARNQLSAHAIDLAEGRKPWDGEVVSRWFAVGGDFRGGYSSVDVPG
                     FAQQSDTDVTRATVYAELRAIPNLLSVYVDQKIAPDDSENREAYLKVTPFDGKLTIKA
                     GQMFVPFGLRLQDDNTFVRQATGVNFLTPDDGVEVGLELAKWSTQLAVIEGPSGADDQ
                     VSATAVYVQPKWRVGLSVNTSEDAFGDREMQSVFAGLKTGPISWLAELSLVNDEAPAG
                     EREFYATLLEGNYRLRKGHNLKLGYEYLEPNRDRDEDEQERYSLVWEYNPIQFVQSRV
                     GVRRYNGIPNIAVSNRDEVFAELHVYF"
     gene            complement(3123..4247)
                     /locus_tag="VM692_09560"
     CDS             complement(3123..4247)
                     /locus_tag="VM692_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52456.1"
                     /translation="MLLTACGSGDGFVGSGGASGPLTANFDSIQANIFEPLCSHCHSG
                     ANAPAGLRLDAANSYALLVGTTAVERPNSKRVAPGDPNSSYIINKLEGGPNIVGERMP
                     AGLPALPQADINVIRQWITDGAQPSSSAAGPIRVTSLSPTPNSTVLALPASITVGFDR
                     EVNAPSVTTASFTLTRAGTDGALGTADDVAIVPASVTVPAANPRSAVMSLAGVASVLD
                     RYRITLVGTGGAAILDLNGNALDGEPIRLLPSGDGTAGGDYTATFTVAGTLPTLTSIQ
                     ANVLTPRCSGCHNGSSANLPGAMNLTNEAASRAALVGIASLEQPTLQRVTAGNPNNSY
                     LIRKLEGTPGIVGNRMPLVGPPLPQATIDAIRLWITNGAQ"
     gene            complement(4449..>6190)
                     /locus_tag="VM692_09565"
     CDS             complement(4449..>6190)
                     /locus_tag="VM692_09565"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HUQ52457.1"
                     /translation="PDTGVPGDLMHTRAAARRSTLLALSLVVPFVWQHAANAQAQALT
                     GRVTSSAEGAMEGVLVSLKREGSNKTVTVVSHADGTYAFPRERLEPGRYAVTTRAVKY
                     ALAERGKTVDIAAGNTAKLDLTLRDSNALELALQMTDPEWLASWPLDDRTKWDLFRDC
                     SRCHTLRRPSMSTYDAEELQWVMQRMVYSAGSSPMRYQLPANAVPHWGRAEMGQPSAL
                     QKRQAEAVAQINLRSGMWSYDLKTMPRPKGKETDVIYTTWDLPATARPHDTRIAADGT
                     IFFNHFNDNAIGRLDPKTGEVKEWHWPYRAKEGSFEPTGARTLMGPDQDGRWYIGNQA
                     QSGVVVFDPKTEQFEYHDPPGGGEMIDVSGSHVDGKAWRAVAGPVVGGGVYQIDLKTW
                     EHVEIKGTTEKPLYAYDIAADLHNNVYGSARNAPYVWRVDAKSHAVQYFDIPAEPRGA
                     GSFGTGMRRGITDSQDRLWWGGFDGGFIGLLDPRKPEGQQMKLYAVPFPYFFPYDAHY
                     DEHGYTWTGGIYADRVARMNVESGEWNFYLLPFEANIRDINLQPATRDGLSGLWIGHT
                     HQAMITLVEPLAR"
BASE COUNT          956 a         2038 c         2128 g         1068 t
ORIGIN      
        1 ggccgtcgtc gccgcggccg cgcgcgccgc ggcgcagccg cccgcgcccg ggttcgccga
       61 cgcgttgctc ggccgctggg atctgacggt cgaagcggcc gacggcgcct atccgtcgtg
      121 gctcgaggtg cggctgcgga cggaagggtc gttgatgggc cggttcgtcg gccgcgtcgg
      181 cagcgcgcgc tacgtcgacg acgtcacgtt cgcggcgggc cgcgtcgcat tcagcgtgcc
      241 cgtgcaatac gaaagcggca tcgacgaggt gcgcttcgaa ggtgtgctgc acggcgagcg
      301 catcgaaggc acggcgcgca tcgacgacgg cgcgacggcg cgcttcacgg ccgtgcgcgc
      361 gccggcactg acgcgctcga cggcccgcga gctcggcgcg ccgacgccgc tgttgaacgg
      421 ccgcgatctg acgggctggg cgccgcggag ccaacagcac gcaggctgct ggcggatgca
      481 gggcggcgtg ctcgccgcga cgccgccgtg cgtcgatctc gtgacgacgg cgacgttcga
      541 cgacttcagg ctgcacgcgg agctgcgcta cccgcgaggc agcaacagcg gcgtctatct
      601 acgcggccgc tacgaggtgc agatccaaga cgacgccggg aaagcgctcg accccctgcg
      661 catcggtggt ctgtacggtt tcgtcgcgcc gtcggccgac gccgcgctac gggccggcga
      721 gtggcagacg ctcgacgtcg agctcgtcgg ccgccgtgtg acggtgacgt tgaacggcac
      781 gacgatcatc gacgcgcagg aaatccccgg catcacgggc ggcgcgctcg acagcgacga
      841 gagcgcgccg gggccgatca tgctgcaagg cgatcacggc gcgatcgagt tccgcaacgt
      901 gacgatcgcg cctgcgcgct gacgcgggcg cgctacggca gcggctcgag cgacgtcaga
      961 tacgcggcga gattcaagat gtcttcggca ccgaggctcg cgacgacggg ctccatcagc
     1021 ggcgtccatg cgccgtggcg tttgccttcc ttcatgtcgg cgagctgccg cgcgatgtag
     1081 ctcgggctgc ggccgcgcaa gctcggcacc atcgcgaggc cgttcaagtc ctggccgtgg
     1141 cagacggtgc acgccgtcac gccttcattg ccgaagcgtg ccagctcttc gccgcgcttg
     1201 acggaacctt tcggcacgta ggcgatgaag ccggagcgcg gattgcgcaa cagctctgtg
     1261 tgctcggcat ttacgggcgt ttcgacgatg cgctgcccaa gcggctccgt gccggcctcg
     1321 gcgccttcga gcttcaagtg catgccgccg gcgatgcgcg tcttaggcac cgtgtcggtt
     1381 tcgacgactt gaatccacgg cgtccacttc atcgagccga agtagtcggc ggccgcgcgg
     1441 atctcttcgt cggtcatctg cacggcgaag ccggccatga tcggcgtgtt cggcttgcgc
     1501 gcatcggcgc tcatgcgagc gccgttcttg aagtcgtaca tctgctggat gaaatactct
     1561 ttcggcaaac cggcgacgcc tgcgttctcg ggccggccct tgccgttcgg gtaatggcac
     1621 agcgagcacg cccaaacgcc ggcggcttcg cggcctttcg cgacgatcgg cggcatggtc
     1681 gggtgatcgc cggggtacca atcggccggt cccatgcggt tgctaatttg ggttcgcgtg
     1741 aatttaccgg tgctaccggg cagcgagtgc aacgtgccgt cgtcgggcgt cgccggcggc
     1801 gcgggacccg gcgtaattgc gtaggcccac gcgagttgct cggcgagcgt ctgcgcggac
     1861 gcaaccgcag gcgccgccgc cgccaacaaa gcggccagcg acgcgccgca tcgtgtcatc
     1921 gaccccatgt gcttcccctc gagcgctctc ggcgcctttt tcggccgagt atagaggatc
     1981 gggtcgcgct gcgaagctag agaccgcggg gttcggctgg gcgacgtcag aaatagacgt
     2041 gcagctcggc gaacacctcg tcgcggttgc tgaccgcgat gtttgggatg ccgttgtagc
     2101 gacgcacgcc gacgcgcgac tgcacgaact gaatcgggtt gtactcccag acgaggctat
     2161 agcgctcctg ttcgtcctcg tcgcgatcgc gattgggctc gaggtactcg tagccgagct
     2221 tcaggttgtg gcccttgcgc aggcgatagt tgccctcgag cagcgtcgca tagaactcgc
     2281 gctcgccggc cggcgcctcg tcgttgacga ggctgagctc ggcgagccac gagatcggtc
     2341 cggttttcag acccgcgaac acgctctgca tctcgcggtc gccgaacgcg tcctcgctgg
     2401 tgttgacgct caagccgacg cgccatttcg gctgcacgta gacggccgtc gcgctgacct
     2461 gatcgtcggc gcccgacggg ccctcgatga cggcgagctg cgtcgaccac ttggcgagct
     2521 cgagcccgac ctcgacgccg tcatcgggcg tcaggaaatt cacgcccgtc gcttgccgca
     2581 cgaacgtgtt gtcgtcctgc aggcgcaggc cgaacggcac gaacatctga ccggctttga
     2641 tcgtgagctt gccgtcgaac ggcgtgacct tgaggtacgc ctcgcgattc tccgaatcgt
     2701 cgggcgcgat cttctgatcc acgtacacgg agagcagatt cggtatcgcg cgaagctcgg
     2761 cgtagacggt cgcgcgcgtc acgtcggtgt ccgactgctg cgcgaaaccc ggcacgtcga
     2821 ccgacgagta gccgccgcga aaatcgccgc cgacggcgaa ccagcggctc acgacctcgc
     2881 cgtcccacgg cttgcgccct tcggcgagat cgattgcgtg cgccgagagc tggttgcgcg
     2941 cgtacagcgt gccgaacgca ttgcgcttgc cgccgccgct cgggttcacg tggcaattgc
     3001 cgcacttgag cccgctctcg acggcgagat acggctcggc agttgcgacg ctcggagcga
     3061 tcgcgcaggc gagcagcgct gcggcgacga tgattgcaaa tgggtgcttc atgtcgttcc
     3121 ctctactgag cgccgttcgt gatccacagc cgaatcgcat cgatcgtcgc ctgcggtagc
     3181 ggcgggccga cgagcggcat gcgattgccg acgatgcccg gcgtcccctc gagcttgcgg
     3241 atcagatagc tgttgttggg gttgccggcg gtcacgcgtt gcagcgtcgg ctgctcgagg
     3301 ctcgcgatgc cgacgagcgc ggcacggctc gcggcctcgt tcgtcaagtt catcgcgccg
     3361 ggcagattcg cactgctgcc gttgtgacaa cccgagcagc gcggcgtcag cacgttcgct
     3421 tgaatcgacg tcaacgtcgg caaggtgccg gccaccgtga acgtcgccgt gtagtcgccg
     3481 cccgccgtgc cgtcgccgga cggcagcaag cgaatcggct cgccgtcgag cgcgttgccg
     3541 ttcaagtcga ggatcgccgc tccgcccgtg ccgacgagcg tgatgcgata gcggtcgagc
     3601 acggacgcga caccggcgag gctcatgacg gccgagcgcg ggttcgccgc gggaacggtg
     3661 acggaggccg gaacgatcgc gacgtcgtcc gccgtgccga gcgcgccgtc ggtccccgcg
     3721 cgcgtcagcg tgaacgacgc ggtcgtgacg gacggtgcgt tgacctcgcg gtcgaagccg
     3781 acggtgatgg aagccggtag cgcgagcaca gtcgagttcg gagtaggcga cagcgacgtg
     3841 acccggatcg gtcccgcggc cgacgagctc ggctgcgcgc cgtcggtgat ccattggcgg
     3901 atcacgttga tgtcggcttg cggcaatgcc ggcaagccgg ccggcatgcg ctcgccgacg
     3961 atgttcggtc cgccttcgag cttgttgatg atgtagcttg agttcggatc gccgggcgcg
     4021 acgcgcttcg aattcggacg ctcgacggcc gtcgtgccga cgagcagcgc gtagctgttt
     4081 gcagcgtcga gccgcaagcc cgcgggcgcg ttcgcgcccg agtggcagtg cgagcagagc
     4141 ggctcgaaga tgttcgcttg tatcgagtcg aagttcgccg tcagcggtcc gctcgcgccg
     4201 ccgctgccga cgaagccgtc gccggagccg catgcagtga gcagcacgct ggctccaaaa
     4261 aggaggaggg taggcacaaa gaggcgcgtc gtcgcgcgcc aaagtttggc agcacgcatg
     4321 ttgctttctc gcaatcgtct tggtgctcgt gagtgccgag cacggcgcag aaaggttcac
     4381 gcgcgcaaaa aaagacgagc gcgcgcacgt ggtgtgcgcg cgacattgtg atcagcagga
     4441 aagaatcgtc agcgcgcgag cggctcgacg agcgtgatca tcgcttgatg cgtgtgaccg
     4501 atccacaacc cggataatcc gtcgcgcgta gcgggctgca aattgatgtc gcgtatgttg
     4561 gcttcgaacg gcaacagata aaaattccat tcgccgctct cgacgttcat gcgggcgacg
     4621 cgatcggcgt agatgccgcc cgtccacgtg tagccgtgct cgtcgtagtg tgcgtcgtac
     4681 ggaaagaaat acgggaacgg cacggcgtac agtttcatct gctggccttc gggcttgcgc
     4741 ggatcgagca ggccgatgaa gccgccgtcg aacccgcccc accagagccg atcctgcgag
     4801 tccgtgatgc cgcggcgcat gcccgtgccg aacgagcccg ctccgcgcgg ctcggcggga
     4861 atgtcgaagt actgcacggc gtgcgacttc gcatcgacgc gccagacgta cggcgcgttg
     4921 cgcgcgctgc cgtagacgtt gttgtgcaag tcggcggcga tgtcgtacgc atacagcggc
     4981 ttctcggtcg tgcctttgat ctcgacgtgc tcccacgtct tgagatcgat ctggtacacg
     5041 ccgccgccca cgacggggcc tgcgacggcg cgccacgcct taccgtcgac gtgcgagccc
     5101 gagacgtcga tcatctcgcc gccgcccggc ggatcgtggt actcgaattg ctcagtcttc
     5161 ggatcgaaca cgaccacgcc gctctgcgcc tgattgccga tgtaccaccg gccgtcctga
     5221 tccgggccca tcaacgtgcg cgcgccggtc ggctcgaacg agccttcctt cgcgcggtac
     5281 ggccaatgcc attccttgac ctcgcccgtc ttcggatcga gccggccgat cgcgttgtcg
     5341 ttgaagtggt tgaagaagat cgtgccgtcg gcggcgatgc gcgtgtcgtg cggccgcgcc
     5401 gtcgcgggca agtcccacgt cgtgtagatg acgtcggtct ccttgccctt cggccgcggc
     5461 atcgtcttca agtcgtaact ccacatgccg cttcgcagat tgatctgcgc gacggcttcg
     5521 gcttgacgct tctgcaaggc ggacggctga cccatctcgg cgcgtcccca gtgaggtacc
     5581 gcgttcgccg gcagttgata gcgcatcggc gagctgccgg ccgaatacac catgcgctgc
     5641 atgacccact gcagctcttc ggcgtcgtac gtggacatcg acggacggcg cagcgtgtga
     5701 cagcggctgc aatcgcggaa cagatcccac ttcgtgcggt cgtcgagcgg ccagctcgcg
     5761 agccactcgg gatccgtcat ctgtaacgcg agctcgagcg cattgctgtc gcgcagcgtg
     5821 aggtctagct tcgccgtatt gccggcggcg atgtcgaccg ttttgccgcg ctcggccagc
     5881 gcgtatttca cggcgcgcgt cgtgacggcg tagcggcccg gctcgaggcg ctcgcgcggg
     5941 aacgcgtagg tgccgtcggc gtgactgacg accgtgacgg tcttgttcga gccctcgcgt
     6001 ttcaggctga cgagcacgcc ttccatcgcg ccttcggccg acgacgtgac gcgccccgtc
     6061 aacgcctggg cctgagcgtt cgcggcgtgt tgccaaacga acggcacgac gagcgacaag
     6121 gccagcagag tcgagcgacg cgcggcagcg cgagtgtgca tgagatcccc cggaacgcca
     6181 gtatccggac
//
LOCUS       DATLEE010000445         2296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9941566, whole genome shotgun sequence.
ACCESSION   DATLEE010000445 DATLEE010000000
VERSION     DATLEE010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9941566"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VM692_09570"
     CDS             complement(<1..330)
                     /locus_tag="VM692_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HUQ52458.1"
                     /translation="MQVPLLDLKGQYAPLRTEIEAAIRQVCDEQRFVLGPRVAELEAK
                     VAEYSQTKHGLGLSSGTDALLLALMALDIGVGDEVITSPFTFFATAGVIARLGAQPFF
                     CDIDPATF"
     gene            complement(706..>2296)
                     /locus_tag="VM692_09575"
     CDS             complement(706..>2296)
                     /locus_tag="VM692_09575"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HUQ52459.1"
                     /translation="VAAERATAQGLMGLLSERKVHRLLIKPPALGITRLLLDSAVNRC
                     VRLADVPANAVPLPVAEQPRPPGARHEAAAVPFWMFATAGAALLTGVVVIASVSAGWR
                     SADDVPLAASAPSRAVTLEGAAAAREDVGVDPRDNTELVVVAAASAEPAAHEQADAVV
                     AASYADAEAALLANDYAAARTALAAVRRADPASSRLAFLEAQLERAQRAAVAPRAEMQ
                     PARAAGASTELDSLLTLARASIQRGELLEPRGGSARDYVGRASRLAPQDGTVAAVRTE
                     LAASLLTAAHRALTAGDVSQTSSLVGEARRFGADAKDLTRLESDATRQLAERAARQSS
                     GWLALADARVRQGALLEPVDDSARHYLTLLRDEAAQEEGLAPVWAAFSAALEQRITAA
                     LVAHDWSAAEDGLAALTDAPNAAAAAESLRTQLAAAKLAEHYLAVVAPASELELKERA
                     APVYPLDAARDGVEGWVDVEFIVDETGRPRDFEVVAAEPQGQFEEAALAALAKYRYEP
                     FAVDGRLFARRVGLRIRFTLE"
BASE COUNT          391 a          792 c          768 g          345 t
ORIGIN      
        1 gaacgtcgcg ggatcgatgt cgcagaagaa cggctgcgcg ccgaggcgcg cgatgactcc
       61 agccgtcgcg aaaaacgtaa acgggctcgt gatgacctcg tcgccgacac cgatgtcgag
      121 cgccatcaaa gcgagcagca aagcgtcggt gccggacgac aagccgaggc catgtttcgt
      181 ctgcgagtac tcggctactt tggcctcgag ctccgcgact cgcggcccga gcacgaaacg
      241 ttgctcgtcg cacacctgac gaatcgcggc ctcgatctcg gtgcgcaacg gtgcgtactg
      301 gcccttcaga tcgagtaatg gcacttgcat ggtagcgacg agccccgctg gcgaagaatc
      361 tggccgcaga acaggactgc acgagtgacg gggtcaggct cgttacgtgc ttactgcgtc
      421 gagagagaga gggcgacgct gccatcgctt agcgccccag gaaaccgccg ccgacagacg
      481 ggactaccca tggcttccat cagacattca gcctgacagc gcgccctcaa gaccaaccgg
      541 tgggttgccc tgatgttctc gtggtttgca ggataaccgg gccaacacca gtcgccaaga
      601 ccgtgatgaa atatgtttcc tagtcaatga cttacaatag ccgaccggcg cggcaatcgg
      661 cagcgcccgg cggccggccg aaagcggcct cacggccgcc ggcagtcact cgagagtgaa
      721 ccgaatgcgc agccccacgc ggcgggcgaa caggcgtccg tcgacggcaa acggctcgta
      781 gcgatacttt gcaagcgcag cgagcgctgc ctcctcaaat tgcccttgcg gctcggccgc
      841 gacgacctcg aagtcgcggg gtcgccccgt ttcgtcgact atgaactcga cgtccaccca
      901 gccctcgacg ccgtcgcgcg cggcgtcgag cggatagacc ggcgctgcgc gttctttgag
      961 ctcgagctcg ctcgcaggcg caacgacagc gagatagtgc tccgcgagct tggccgcggc
     1021 gagttgcgtg cgcagcgact cggccgcagc ggcggcgttc ggcgcgtcgg taagcgccgc
     1081 cagcccgtcc tctgccgccg accaatcgtg agcgacgagt gcggccgtta tccgctgctc
     1141 gagcgctgcc gaaaacgccg cccagactgg agcgagcccc tcctcctgcg ccgcctcgtc
     1201 ccgcaacaaa gttagatagt gccgagcgct gtcgtcgacg ggctcgagca gcgcaccttg
     1261 tcgaacccgt gcgtcggcga gtgcgagcca tccggaactt tgtcgtgcgg ctcgctcggc
     1321 aagctggcga gtggcgtcgg actcgagccg cgtgaggtcc ttggcatcgg cgccgaagcg
     1381 acgcgcttcg ccgacgagtg agctcgtttg cgagacgtcg cccgccgtca gcgcgcggtg
     1441 cgcagccgtt agcagcgatg ccgcgagctc cgtgcgtacc gctgcaacgg tcccgtcttg
     1501 cggcgcaagc cggctcgccc gcccaacgta atctcgcgcg cttcccccac gcggctcgag
     1561 cagctcgccg cgctgaatgc tagcgcgcgc tagagtgagg agactgtcga gctccgtgct
     1621 cgcgccagcc gcacgcgcag gctgcatctc agcgcgcgga gcgaccgccg cgcgctgtgc
     1681 gcgctcgagt tgcgcctcga gaaaggccaa gcgactgctc gccgggtcgg cgcggcgcac
     1741 agccgcaagc gccgtccggg ccgcggcgta gtcgtttgca agcagcgccg cttcggcgtc
     1801 ggcgtaactc gcagcgacga cggcgtcggc ttgctcatga gcggctggct cggccgaagc
     1861 cgcggcaacg acgacgagct cggtattgtc ccgcggatcg acgccgacat cttcgcgcgc
     1921 ggctgcggcg ccctcgaggg tgactgcgcg tgagggcgcg cttgctgcga gcggcacgtc
     1981 gtcagccgac ctccaccccg ctgagacgct cgcgatgacg acgacgccag ttagcagcgc
     2041 agcccccgcc gtggcgaaca tccagaacgg cacggccgct gcttcgtgtc gggccccggg
     2101 cggtcgtggc tgctcggcga cgggcagcgg cacggcgttg gcgggcacat ccgcaagcct
     2161 cacgcagcga ttaacggcgg aatcgagcag cagccgcgta attccaagtg ccggcggctt
     2221 gatcaacaaa cggtggacct tgcgctcgga gagaaggccc atgagaccct gcgccgtcgc
     2281 ccgctcggcg gccacc
//
LOCUS       DATLEE010000446         3867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1246163, whole genome shotgun sequence.
ACCESSION   DATLEE010000446 DATLEE010000000
VERSION     DATLEE010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246163"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VM692_09580"
     CDS             <1..1033
                     /locus_tag="VM692_09580"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HUQ52460.1"
                     /translation="FHLTLYEPLAPMLASPTADVDSALAALESAVLEYKLDGARVQIH
                     KGPQGTRVYSRTGREVTASVPEIAAAIAALPAAALILDGEVLALTKAGHPRPFQDTMR
                     RFGRKLAVADMQGALPLSLFCFDCLHLDGEDLIDRPLAERHERLAAAVPAELNVPRLL
                     TRERDAAQAFLAAALKRGHEGIMAKDPASRYEAGSRGAAWLKIKVAHTLDLVVLAAEW
                     GSGRRQGWLSNLHLGARDPNGGFVMLGKTFKGLTDELLEWQTQQLKALALDPAARDYV
                     VAVRPELVVEIAFNELQVSRRYPGGLALRFARVVRYRPDKRPEDADTIETVRAIAAAQ
                     GAVAAEESA"
     gene            complement(1044..1532)
                     /locus_tag="VM692_09585"
     CDS             complement(1044..1532)
                     /locus_tag="VM692_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HUQ52461.1"
                     /translation="MKRSRKAANAKPKAKARTKAAPKAKRPASKSRAKTAAATPAAAQ
                     LIDERIRELAGWRGETLARMRALILEADSAMTEEWKWDVPVWSHGGIVCTGEAYKSVV
                     KLTFARGATVEDPARLFNSSLAGNTRRAIDIREGEQVDAAAFKALVQAAVARNALPAK
                     KP"
     gene            1656..2072
                     /locus_tag="VM692_09590"
     CDS             1656..2072
                     /locus_tag="VM692_09590"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HUQ52462.1"
                     /translation="MKPGLPLIYIAAGLLVGCATTPATTAGDRAAAPANADCLRTSLI
                     RDWDALDERNLIVYESGRRPYHVELTQSCFGLDFSEMLGFYDRRGDGRICGYGLDRVI
                     VERAIPESCSVAAVDELTDEQAVALKERAESARARR"
     gene            complement(2035..2895)
                     /locus_tag="VM692_09595"
     CDS             complement(2035..2895)
                     /locus_tag="VM692_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HUQ52463.1"
                     /translation="MSTTTAEHGGARRQWWDIARRVKDDVAADNVSMIAGGAAFFGLL
                     AMFPALAAGVALYGLFTDPASINTQLDLVGGVLPGEVRTILGEQLQRITSTANSSLGI
                     GAAVALLLALWSAAKGVKSLMVALNVVYHEKERRGFFKLNGTALLLTLTMLLVIPLML
                     AAVAVLPAVVDRLPLPDIAATLARWLRWPLLALVAVGAIGLLYRFGAARTRPRPKWSW
                     PGAIVATVLWLVASALFSWYVSSFGSYNETYGSVAAIAVLMMWFWLSTYAVLLGGEIN
                     AELERSPRAP"
     gene            3023..3442
                     /locus_tag="VM692_09600"
     CDS             3023..3442
                     /locus_tag="VM692_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUQ52464.1"
                     /translation="MKISEVMTPDVSFVMPDTPILEIARKMREADIGSTPVLDNDRLV
                     GMVTDRDIVVRVVAEGRPTQTACARDAMSPGIFFCYEDESIEDVLEKMGDEQIRRLPV
                     VNRAKRLVGVVSLGDLSLTGRRKRAGEALQEISQPSH"
     gene            3468..>3867
                     /locus_tag="VM692_09605"
     CDS             3468..>3867
                     /locus_tag="VM692_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52465.1"
                     /translation="MRSAIVLGVLAAVAIAVAAAMHYGAGRLDGAVASTVERYGAEIT
                     GTDVDVGAVDLALTDGRADLDGITIANPQGYETDYAVRIGHASVSIDVGSLTGDVPVI
                     DELTLDGALINAEQRETASNLTDIQHHATSS"
BASE COUNT          622 a         1333 c         1311 g          601 t
ORIGIN      
        1 tttccacctg acgctgtacg agccgttggc gccgatgctt gcgagcccga ccgcggacgt
       61 cgacagcgcg ctcgcggcgc tcgagtcggc ggtcctcgag tacaagctcg acggcgcgcg
      121 cgtgcaaatt cacaaggggc cgcaaggaac gcgcgtctat tcgcgcacgg gccgcgaagt
      181 gacggccagc gtgccggaga tcgcggcggc gatcgccgca ttgccggccg ctgctttgat
      241 tctcgacggc gaagtgctgg cgctgacgaa ggccggccac ccacggccgt tccaggacac
      301 gatgcgccgc ttcggccgca agctcgccgt cgccgacatg caaggcgcgc tgccgctgtc
      361 gctgttctgc ttcgattgcc tgcacctgga cggcgaggat ctcatcgacc ggccgctcgc
      421 cgagcggcac gagcggctcg ccgccgccgt gccggccgag ctcaacgtgc cgcgcctcct
      481 aacgcgcgag cgcgacgccg cgcaggcgtt cctcgcggcc gcgctgaaac gcggccacga
      541 gggcatcatg gccaaggatc ccgcaagccg ctacgaggcg ggcagccgcg gcgctgcgtg
      601 gctcaagatc aaggtcgcgc atacgctcga tctcgtcgtg ctcgcggccg agtggggtag
      661 cggccgacgg caaggttggc tcagcaatct gcatctcggc gcgcgcgacc cgaatggcgg
      721 cttcgtgatg ctcggcaaga cgttcaaggg tctaaccgac gagctgctcg agtggcagac
      781 gcagcagctg aaggcgctcg cgctcgatcc cgcggcgcgc gactacgtcg tcgcggtgcg
      841 gcccgagctc gtcgtcgaga tcgctttcaa cgagctgcag gtcagccgcc gctacccggg
      901 cgggctcgcg ttgcggttcg cgcgcgtagt ccgttatcgt cccgacaagc ggcccgaaga
      961 cgccgacacg atcgagaccg tgcgcgcgat cgcggcggcg cagggcgccg tcgcggccga
     1021 ggagtcggcg tagtcgaggc gcgttacggc ttcttcgcgg gcagggcgtt gcgcgcaacc
     1081 gccgcctgca cgagcgcttt gaacgcggcc gcgtcgacct gctcaccttc gcggatgtcg
     1141 atcgcccggc gcgtgttgcc tgccaagctc gagttgaaca ggcgcgcagg gtcctcgacc
     1201 gtggcgccgc gagcgaacgt cagcttgacg acgctcttgt aggcttcgcc cgtgcagacg
     1261 atcccgccgt gcgaccagac cggaacgtcc catttccact cctccgtcat cgcggagtcg
     1321 gcctcgagga tcagcgcgcg catgcgcgcg agcgtctcgc cgcgccaacc cgcgagctcg
     1381 cgaatgcgct cgtcgatgag ctgcgccgcc gccggtgtcg ccgcggcggt cttcgcccgc
     1441 gacttcgacg cgggccgctt cgccttgggc gcggctttcg ttctcgcctt ggccttcggt
     1501 ttggcgttgg ctgctttccg gcttcgcttc atagtgtggc tcgcagaggg cgggtgctcg
     1561 gcgaattgtg cccgcggtgc tccgccccgc aaaagatcct aggcggcacg gcctttgcac
     1621 ctgcacagcc ccagccgcga ccgaggagct tcgccatgaa acccggcttg ccactcatct
     1681 atatagcggc cggcctgcta gtcggctgcg cgacgacgcc ggctacgacg gcgggcgacc
     1741 gcgctgcggc gcctgccaac gcggactgcc tgcggacctc gctgatcagg gactgggacg
     1801 cgctcgacga gcgcaatctg atcgtctacg agagcggccg ccggccctat cacgtcgagc
     1861 tgacgcaatc ctgcttcggc ctcgacttct cggagatgct cgggttctac gaccggcgcg
     1921 gcgatgggcg catctgcggt tacgggctcg accgcgtgat cgtcgagcgc gcgatccccg
     1981 agagctgcag cgtggccgcc gtcgacgagc tgaccgatga gcaagccgtt gcgctcaagg
     2041 agcgcgcgga gagcgctcga gctcggcgtt gatctcgccg ccgagcagca cggcgtacgt
     2101 cgacagccag aaccacatca tcagcacggc gatcgccgcg acggacccgt aggtctcgtt
     2161 gtagctgccg aagctcgaca cgtaccacga gaacagcgcc gaggcgacga gccagagcac
     2221 cgtcgcaacg atcgcgcccg gccacgacca tttgggccga gggcgcgtgc gcgccgcgcc
     2281 gaagcggtac agcaggccga tcgcgccgac ggcgaccaac gccagcagcg gccagcgcaa
     2341 ccagcgggcg agggtggctg cgatgtcggg cagcggcagc cggtcgacga cggccggcag
     2401 cacggctacg gccgccagca tcagcggaat gacgagcagc atcgtcagcg tcagcagcaa
     2461 ggccgtgccg ttgagcttga agaagccgcg gcgttccttc tcgtgataga cgacgttcag
     2521 cgcgaccatc agcgacttga cccccttcgc cgcgctccat agcgcgagca gcagcgcgac
     2581 ggcggcgccg atgccgagcg agctgttcgc cgtgctcgtg atgcgctgca attgctcgcc
     2641 gaggatcgtg cgcacctcgc cgggcagcac gccgcccacg aggtcgagct gcgtgttgat
     2701 gctcgcggga tccgtgaaca ggccgtagag cgcgaccccg gccgcgagcg cgggaaacat
     2761 tgcgagcagg ccgaagaacg cggcgccgcc ggcgatcatc gaaacgttgt cggccgctac
     2821 gtcgtccttg acgcgccgcg cgatgtccca ccactggcgc cgtgcgccac catgctctgc
     2881 cgtcgtcgtg ctcatttcga tccatccttg gaacgccgtg tggctgcaag gtgtatgcca
     2941 gccgcgcgtc tgcgcgcagt cgtggcacgc cacttgcacg accattgccc gcccaaaacc
     3001 tactacagcg aggtctagtg caatgaaaat tagcgaggtc atgacgccgg atgtcagctt
     3061 cgtcatgccg gacacaccaa tcctggaaat cgctcgcaaa atgcgcgaag ccgacattgg
     3121 ctcgacgccg gttctcgata acgaccgcct cgtcggcatg gtcacggacc gcgacatcgt
     3181 cgtgcgcgtc gtcgccgagg gtcggccgac gcagacggcg tgtgcgcgcg atgcgatgtc
     3241 gcccggcata tttttttgct acgaggacga gtcgatcgaa gacgtgctcg agaagatggg
     3301 cgacgagcag atcaggcgct tgcccgtcgt caatcgcgcc aagcggctcg tcggcgtcgt
     3361 gtcgctcggc gacctgtcgt tgacgggccg acgcaagcga gccggcgaag cgttgcaaga
     3421 gatctcacag ccgtcgcact gaggctaaca actcgcgagg caccaccatg cgttcagcga
     3481 tcgtgttggg agtcttggcg gccgtcgcga ttgcggtggc tgcggctatg cattacggcg
     3541 ccggccgcct cgacggcgcg gtggcctcga cagtcgagcg ttatggcgcc gagataacgg
     3601 gcacggacgt cgacgtcggc gccgtcgacc tcgcgctcac cgacggccgc gccgatctcg
     3661 acggcatcac gatcgcgaac ccgcagggct acgagaccga ctacgccgtg cgtatcggtc
     3721 acgcgagcgt ctcgatcgac gtcggctcgt tgaccggcga cgtgcccgtc atcgacgagc
     3781 tgacgctcga cggcgcgctg atcaacgccg agcagcgcga gaccgcgagc aatctcacgg
     3841 acatccagca tcacgcgacg tcgtcgt
//
LOCUS       DATLEE010000447         9014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4207053, whole genome shotgun sequence.
ACCESSION   DATLEE010000447 DATLEE010000000
VERSION     DATLEE010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4207053"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VM692_09610"
     CDS             <1..1227
                     /locus_tag="VM692_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52466.1"
                     /translation="EELLVASQDEALHGNNRYYPQAWAFVHYLMFGNGGTQAAKLTTF
                     LREQQRTDLNTAFTTAFDKSYSDFNAEIRRYLQGGRYAYAEVPLRDNSREMEISPASE
                     ASVELALGRLATAGGNQELALAHAERVIALASTSPAGYELKAYGAVDIDDGAAFDAAL
                     ERAIALGSRDSFLYEAKAARLINANQRTNSSVDDLFDADVARTAADLFQRALGLRPRN
                     DTPLRGLVVALLNVETHTDTDQLTLNAGRVMFPTNGLLLVGQAALEKQRGNVSEASRL
                     LQQATVEPFTLTSEDRRMIVALRGNWLGQWVAAQLNELVPAGRFEEVRALVAEQLADE
                     TLPGPLRSMIERVQKDLPEMERLDEAAQAAQNGRRAEAVAILTELADAPDTGERARRV
                     AERMLRDFGVRGAAPN"
     gene            1394..1960
                     /locus_tag="VM692_09615"
     CDS             1394..1960
                     /locus_tag="VM692_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52467.1"
                     /translation="MKRTALFGAVLAAFGPGLAFADMNYTKFEISMLDVEMDSTVNVD
                     GDGFALGGSYELNDKIFLLGEWQDQSLDFGIDGRTLELGAGYTHGMNDKLDFVGTLSY
                     VDAEVKAGNLTADVDGLALGGGIRTRIAQSVELDAGLKYVDFDNAGGDTGFSVGGRYY
                     FNDSMALGGSADFNDNADTLRVSFRWEF"
     gene            complement(2033..2530)
                     /locus_tag="VM692_09620"
     CDS             complement(2033..2530)
                     /locus_tag="VM692_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52468.1"
                     /translation="MQNVVFVLAALAASAALAQEAAAPPLVTGADEVIVRGRAGPKLR
                     VELERVENAVYERFNALNSRDELDIVCMNDAPLGSHMPVRECLPKFALTAERRAAQDI
                     LHRMQGGHFAGSTQIHFMDMEEKGQELAAEMQRLAREDEQLLRELTRLAELKQSLGDR
                     VGKRR"
     gene            complement(2708..3307)
                     /locus_tag="VM692_09625"
     CDS             complement(2708..3307)
                     /locus_tag="VM692_09625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HUQ52469.1"
                     /translation="MAAPVFKDDLVTRREAIRRVSALLGGAALIGQSAWLTGCSEGLS
                     ASRALFPAHDVALLDEIADTILPETKTPGAKAAGVGAFIATMVTDTYDPREQRVFRDG
                     LEELERESKKQNGGGFMASSPAQRLALLERIDREAIEFLRGNFDRPHYFRMIKELTLL
                     GYFTSEIGYTQAQRYVESPGRFDACITYTVGDKAWAPHA"
     gene            complement(3327..5012)
                     /locus_tag="VM692_09630"
     CDS             complement(3327..5012)
                     /locus_tag="VM692_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007106336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HUQ52470.1"
                     /translation="MANENYDAIVVGSGISGGWAAKELTEKGLTVLLLERGKNIEHAK
                     DYVNAHKGPWEYAHRGNRTYAMEEAYQVLSRDYPLNEKNLDWWVNEKDSPYTEVKRFD
                     WYRGYHVGGRSLLWGRQSYRWSDFDFEANAREGIAVDWPVRYAELAPWYDYVERHAGI
                     SGSIEGLAQLPDGQFQPAMPLNCGEELVAGRLRGRFDGKRRLISGRVANLTQPLPGRS
                     PCQYRSACWLGCPYGAYFSTQSSTLPAAMATGRLTLKPFSIATEVLYDKDKKRATGVR
                     VLDAVTEQTTEYTARVVFVNASTLNSAWLLMNSATDVWPGGLGSSSGELGHNLMDHHF
                     RAGAEGFLVDEVLNGQYYFGNRPTGFYIPRYRNLFGEKREYLRGFGYQGSASRQGWQR
                     AVREAGVGGAVKDRAAEPGAWQIGGLAFGEILPRHDNQVSLDKMKKDKWGLPVLAIDC
                     ETGENERKMREDMMNDMAEMLEACGVRSVTTFDRGYAMGMGIHEMGTARMGNDPKTSV
                     LNRWNQVWDAPNVFVTDGSCMVSSSCVNPSLTYMALTARAVDHAVGELSRRNI"
     gene            5140..6300
                     /locus_tag="VM692_09635"
     CDS             5140..6300
                     /locus_tag="VM692_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HUQ52471.1"
                     /translation="MVGGGEGAFIGAVHRSAAGLDNALELVCGAFASDAERSRRSGAN
                     LGLPPERCYADFNSMFAAEAKLPADARMEFVAIVTPNNLHLAVASAAFKHGFHVLSDK
                     PATTTLAECFELRDALKVSGCLYGLTHPYGSYPLIVEAAERVRAGALGTIRKVIVEYT
                     QGWLAEPIERRGQKQAIWRLDPKRAGLSSCMGDIGVHAFHLAEFVTNLRTTELCADLN
                     SIVAGRELDDDGAAFLRFDNGARGVLVASQICTGEENDLRLRVYGDVASLDWRQEEPN
                     SLWLKHAHKPIELIRSGGTNFGAAARAHSRLPAGHPEGYLEAFANVYRNFAAQVRAHG
                     GTNGGPAPRAVPSIKAALRGMAFIETVVAASKSAQKWHAFPNVTDDGLPRAT"
     gene            6278..7153
                     /locus_tag="VM692_09640"
     CDS             6278..7153
                     /locus_tag="VM692_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUQ52472.1"
                     /translation="MGSLERHDNRAVLFADISGSTKLYVEVGDERAREIVARTLELWS
                     ALTVEGGGNVIQLRGDGMLCMFPSVDAALLAVVRMRDLPYQAPLSMHAGLHVGVVLQE
                     SEQLYGDVVNVAARMADVAKKFEVVLTAEAQRQIAQPARWPNLRLIRQVPIKGKPEPM
                     DVYLLANDRRGLTDYQPPLKKATSGQLHLRYAGRLIAVDIQTGACLIGRDDDCRIKIE
                     HPRVSRRHASVECAAGKFFLHDHSTNGTYVAERQTAPVLVHRELLQLKESGVISLGIE
                     PQLNVDHSIVFSFEK"
     gene            complement(7173..7817)
                     /locus_tag="VM692_09645"
     CDS             complement(7173..7817)
                     /locus_tag="VM692_09645"
                     /inference="COORDINATES: protein motif:HMM:NF040152.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HUQ52473.1"
                     /translation="MSTELASAADANEQPAKVDKPDKPGKSDKEKKEKRSERRRGLET
                     LFRVTYRNHISLSQLADQKAGMLISINGLIISVLIAFVAPRAGAWSWTLAPALVLVAG
                     CVSSLAFAVIAARPRVDRTPVTVEEVRNNQANLLFFGHFLSMPLADFQAAIRALGKDR
                     KLLHDSLTRQLYSMGEALTAKYRCLQLAYTLFLIGVATAAVLFAALFVASAASA"
     gene            8103..>9014
                     /locus_tag="VM692_09650"
     CDS             8103..>9014
                     /locus_tag="VM692_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52474.1"
                     /translation="MRCTASSLLAIRSRLYAAAALAAAVTSSASADLFATDEPLALTL
                     SAPFGAVFRATTEDPEYQDATLAYRSADGSAASVPLRVRVRGKSRAAVCDFKPLLLNF
                     RTADLVGTAFEGEDRLKLVTHCKASASYDEYLKLEYLSYRVWSLLSETALRARLIEVA
                     YVDSSRARDLGTRPGILLEDEGRFAERQALTVFEGPKVERAKYDAGALALLDVFQYFV
                     GNTDWSALAGPAGQECCHNVVPYVRADGVMVPVPYDFDATGLVNAPHALPDERLPIRS
                     VRQRLYRGACRTPEELAPSFERFQAQRA"
BASE COUNT         1519 a         3047 c         2934 g         1514 t
ORIGIN      
        1 gaggagctgc tcgtcgcttc gcaggacgaa gcgctgcatg gcaacaacag gtactaccca
       61 caggcgtggg cgttcgttca ctacctgatg ttcggcaacg gcggcacgca agcagcgaaa
      121 ttgacgacct ttctgcgcga gcagcaacga accgatctga acacggcttt cacgactgcg
      181 ttcgacaagt cttattcgga cttcaacgcg gaaatccgcc gttacctgca aggcggccgc
      241 tacgcgtatg cggaagtgcc gctgcgcgac aacagccgcg agatggagat ctcgccggct
      301 tcggaggcga gcgtcgagct cgcgctcgga cgcttggcga cggccggagg aaatcaggag
      361 ctcgcgctcg cacatgccga gcgcgtcatc gcgctcgcct cgacgtcacc tgccggctac
      421 gagctgaaag cctatggcgc agtcgacata gacgatggcg cggcattcga cgcggcgctc
      481 gagcgcgcaa tcgccctcgg ctcgcgcgac tcatttctct acgaggcgaa ggcagcgcgg
      541 ttgatcaatg cgaaccagcg caccaatagc tccgtcgacg acctcttcga cgccgacgtt
      601 gcgcgcacgg ccgcggacct tttccagcgc gcgctgggcc tgcgcccacg caacgacaca
      661 ccgttgcggg gcctggttgt ggcattgctg aacgtcgaaa cgcacacgga cacggaccag
      721 ctcacactga acgcgggccg cgtcatgttt ccgacgaatg gcctgctgct cgtagggcaa
      781 gcggcgttgg agaaacagcg cggcaacgtc agcgaggctt cgcgtctact gcagcaggcg
      841 acggtggagc ccttcacgct gacaagcgag gatcgacgca tgatcgtcgc attgcgaggc
      901 aactggctcg ggcagtgggt tgcggcccag ctcaacgagc tagtgccggc aggacgcttc
      961 gaggaggtcc gggcgctcgt tgccgagcaa ctcgcggacg agacgcttcc gggaccgctg
     1021 cgctcgatga tcgagcgtgt ccagaaagat ctgcccgaga tggaacgtct cgacgaagct
     1081 gcgcaagcgg cccagaacgg ccggcgcgcg gaggccgtag cgatcctcac ggagctcgcg
     1141 gatgccccgg acaccgggga acgcgcgcgg cgcgtcgccg agcgcatgtt gcgcgacttc
     1201 ggcgtgcgcg gcgcggcgcc aaactgagcc aagcttcggg cgtcatctgt taaatcgcga
     1261 ggcgctgcga agcgtcgcga cataacgcgc gtgttgtctt tgcaacaacc gcgcgagctg
     1321 tgacgcgagc ctcagccgag gctcgatcgg ccccaatacg atgccgccgc gcattcactt
     1381 gggagatcgt tttatgaaac gcactgcact ctttggcgcc gtactggcgg ccttcggtcc
     1441 gggccttgca ttcgccgaca tgaactacac gaagttcgag atcagcatgc tcgacgtcga
     1501 gatggactcg accgtgaatg tcgacggcga cgggttcgcg ctcggcggct catacgagct
     1561 gaacgacaag atcttcttgc tcggcgagtg gcaagaccag agcctcgact tcggaatcga
     1621 cgggcgcacg ctcgagctcg gtgccggtta cacgcacggc atgaacgaca agctcgactt
     1681 cgtcggcacc ctgtcgtacg tcgacgcgga agtgaaggcc gggaacctga cggctgacgt
     1741 cgacggcctc gcgctcggcg gcgggatccg cacgcgcatc gcgcagtcgg tcgagctcga
     1801 tgccggcctc aagtacgtcg acttcgacaa cgcgggcggg gacaccggct tctcggtcgg
     1861 cggtcgttac tacttcaacg acagcatggc cttgggcggc agcgccgact tcaatgacaa
     1921 cgccgacacg ttgcgcgtga gcttccgctg ggagttctga gcacgcgtca caaggcgagt
     1981 tagtgggaat ggcggcggcc cgcaacgggc cgccgctttt ttttggggaa ccttagcgtc
     2041 gcttgccgac gcgatcgccg aggctctgct tgagctcggc gaggcgagtc agctcgcgca
     2101 gcagctgctc gtcttcgcgc gcgaggcgct gcatctccgc ggccagctct tggcctttct
     2161 cttccatgtc catgaagtgg atctgcgtgc tgccggcgaa gtgcccgcct tgcatgcggt
     2221 gcaagatgtc ttgcgcggcg cgccgttccg ccgtcagcgc gaacttcggc aggcactcgc
     2281 gcacgggcat gtgcgaccct agcggcgcat cgttcatgca aacgatgtcg agctcgtcgc
     2341 ggctgttcag cgcgttgaaa cgctcgtaga cggcattctc gacgcgctcg agctcgacgc
     2401 gcagcttggg ccccgcgcgg ccacgcacga tcacctcgtc ggcgcccgtg acgagcggcg
     2461 gcgcggcggc ttcttgagcc agcgcggccg acgcggcgag cgcggcgaga acgaacacga
     2521 cgttctgcat gcgtcgaagc ttagcggaac accgcagccg tgacgacgcc ccttggaagt
     2581 ccccgaaggg ggacaaccct tggaagtccc cgaaggggga cgacccttgg tagtccccga
     2641 aagggggacg ccccttgacc gcagcgccgt ctcaagcgtc gccgagccgc cgctgccgcc
     2701 gcagcgatca ggcgtgcggc gcccaggctt tgtcgccgac ggtgtacgtg atgcaggcgt
     2761 cgaatcgccc ggggctctcg acgtaacgct gcgcttgcgt gtagccgatc tcggacgtga
     2821 agtaaccgag cagcgtcagc tctttgatca tccgaaagta gtgcggccga tcgaagttgc
     2881 cgcgcagaaa ctcgatggcc tcgcggtcga ttcgctcgag cagcgccagg cgctgcgccg
     2941 gcgagctggc catgaagccg ccgccgttct gcttcttcga ttcgcgctcg agctcttcga
     3001 gcccgtcgcg aaacacgcgc tgctcgcgcg gatcgtaagt gtccgtgacc atcgtcgcga
     3061 tgaacgcccc gactccggcg gccttcgcgc ccggcgtctt ggtctcgggc aggatcgtgt
     3121 cggcaatctc gtcgagcagc gcgacgtcgt gtgccgggaa caatgcgcgc gaagcgctca
     3181 agccctctga gcaacccgtc agccaggcgc tctgccctat cagcgccgcg ccgccgagca
     3241 gcgcgctgac gcgccggatt gcttcgcgcc gcgttacgag atcgtctttg aataccggtg
     3301 cagccattcg agactccgag actccgttag atattgcgcc gcgacagctc gccgacggcg
     3361 tgatcgacgg cgcgcgccgt caacgccatg tacgtcagcg aggggttcac gcacgacgac
     3421 gacaccatgc acgagccgtc ggtcacgaac acgttcggcg cgtcccagac ctggttccag
     3481 cgattgagca ccgacgtctt cgggtcgttg cccatgcgcg ccgtgcccat ctcgtggatg
     3541 cccatgccca tcgcgtaacc gcggtcgaac gtcgtcacgc tgcggactcc gcaggcttcg
     3601 agcatctcgg ccatgtcgtt catcatgtct tcgcgcatct tccgctcgtt ctcgccggtc
     3661 tcgcagtcga tcgccagcac gggcaagccc cacttgtcct tcttcatctt gtcgagcgac
     3721 acctggttat cgtgccgcgg caagatctcg ccgaacgcga ggccgccgat ctgccaagct
     3781 cccggctccg cggcgcgatc cttgaccgcg ccgccgacgc cggcctcgcg cacggcgcgc
     3841 tgccagccct gccggctcgc gctgccttgg tagccgaagc cgcgcagata ctcgcgcttc
     3901 tcgccgaaca aattgcggta gcgcggaatg tagaagcccg tcggccgatt gccgaagtaa
     3961 tactggccgt tcaacacctc gtcgacgagg aagccttccg ctccggcgcg gaagtggtgg
     4021 tccatcaagt tgtggccgag ctcccccgag ctcgagccca aaccgccggg ccagacgtcc
     4081 gtcgcggagt tcatcagcag ccacgcgctg ttcagcgtcg acgcgttgac gaacacgacg
     4141 cgcgccgtgt actccgttgt ctgctccgtg acggcgtcga gaacgcgcac gcccgtcgcg
     4201 cgcttcttgt ccttgtcgta cagcacctcg gtcgcaatcg agaacggctt cagcgtgagc
     4261 cgccccgtcg ccattgcggc cggcagcgtc gaggactgcg tgctgaagta agcgccgtag
     4321 gggcagccga gccagcaagc gctgcggtac tggcacggcg agcggcccgg caacggctgc
     4381 gtcaggttcg cgacgcggcc cgagatcagg cgccgcttgc cgtcgaaccg gccacgcaac
     4441 cgcccggcga cgagctcttc gccgcagttc agcggcatcg cgggttggaa ctgcccgtcc
     4501 ggcagctgtg ccagaccctc gatcgagccc gaaatgccgg catgccgttc gacatagtcg
     4561 taccacggcg cgagctcggc gtagcgaacc ggccaatcga cggcgatacc ttcgcgcgcg
     4621 ttggcttcga aatcgaaatc gctccagcga tagctctgcc gcccccagag cagcgagcgc
     4681 ccgccgacgt gatagccgcg gtaccaatcg aagcgcttga cctcggtgta cggcgaatcc
     4741 ttctcgttga cccaccagtc gagattcttc tcgttgagcg ggtagtcgcg gctcaacacc
     4801 tggtacgcct cttccatcgc gtaggtgcga ttgccgcggt gcgcgtactc ccacgggccc
     4861 ttgtgggcgt tgacgtagtc cttggcgtgc tcgatgttct tgccgcgctc gagcaacagc
     4921 accgtcaaac ctttttccgt cagctctttg gccgcccagc cgccggatat accggagccg
     4981 acgacgatgg cgtcgtaatt ttcgttggcc actcttctga gaccccccgt gcgtgatcgg
     5041 gcggatgtta ctgcatgcgg tcgggctctg gaaccgacgt tcgcggcgac tgagcggtct
     5101 tacgctagaa tcgcgctcgt gagaaaagtc agattcggca tggtcggcgg cggcgaaggc
     5161 gcattcatcg gcgccgtgca ccgctcggcc gcgggcttgg acaacgctct cgagctcgtc
     5221 tgcggtgcgt tcgccagcga cgcagagcgc agccgccgca gcggtgcgaa cttggggttg
     5281 ccgcccgagc gctgctacgc cgacttcaac tcgatgttcg cggccgaagc caagctgccc
     5341 gccgacgcgc gcatggagtt cgtcgcgatc gtcacgccga acaacttgca cttggccgtc
     5401 gcgagcgccg cgttcaagca cggctttcac gtgctgtcgg acaagcccgc gacgacgacg
     5461 ctcgccgagt gcttcgagct gcgcgacgcg ctgaaagtca gcggctgcct ctacggcctc
     5521 acgcatccgt acgggtcgta cccgttgatc gtcgaagccg cggagcgcgt gcgcgcgggc
     5581 gcgctcggca cgatccgcaa agtcatcgtc gagtacacgc agggctggct cgcggaaccg
     5641 atcgagcggc gcggccagaa gcaagccatc tggcgcctcg acccgaaacg cgcgggcctc
     5701 tcgagctgca tgggcgacat cggcgtccac gcgtttcatc tcgccgagtt cgtcacgaac
     5761 ttgcgcacca cggagctctg cgcggacttg aacagcatcg tcgccggccg cgagctcgac
     5821 gacgacggcg ccgcgttcct gcgcttcgac aacggcgcgc gcggcgtgct cgtcgcgagc
     5881 cagatctgca ccggcgagga gaacgacttg cgcttgcgcg tctacggcga cgtcgcgagc
     5941 ctcgactggc gccaagagga gcccaactcg ctgtggctca agcatgcgca caagccgatc
     6001 gaattgatcc gcagcggcgg cacgaacttc ggcgcggcgg cacgcgcgca ctcgcgcttg
     6061 ccggccggcc accccgaggg ctatctcgag gcgttcgcga acgtctaccg caacttcgcg
     6121 gcgcaagtgc gcgcgcacgg cggcacgaac ggcggccctg cgccgcgggc ggtgccgagc
     6181 atcaaagcgg cgctgcgcgg catggcgttc atcgagacag tcgtcgcggc gagcaagtcc
     6241 gcgcagaaat ggcacgcgtt tccgaacgtc acggacgatg ggctccctcg agcgacatga
     6301 caaccgcgcg gtgctgttcg ccgacatcag cggcagcacg aagctctacg tcgaagtcgg
     6361 cgacgagcgc gcccgcgaga tcgtcgcccg cacgctcgag ctttggtcgg cgctgaccgt
     6421 cgaaggcggc ggcaacgtca tccagctgcg cggcgacggc atgctctgca tgtttccgtc
     6481 cgtcgacgcg gcgctcctcg ccgtcgtgcg catgcgcgat ttgccgtatc aagcgccgct
     6541 gtcgatgcac gcgggcctgc acgtcggcgt cgtgctgcaa gagtcggagc agctctacgg
     6601 cgacgtcgtc aacgtcgcgg cgcgtatggc cgacgtcgcc aagaagttcg aggtcgtgct
     6661 aacggccgag gcgcagcggc agatcgcgca gcccgcgcgc tggccgaatt tgcgtttgat
     6721 ccgccaagtg ccgatcaagg gcaagcccga gcccatggac gtttacctgc tcgcgaacga
     6781 ccgtcgcggt ttgacggatt accagccgcc gctgaagaaa gcgacgtcgg ggcagctgca
     6841 cctgcgctac gcgggccggc tcatcgcggt cgacatccag acgggcgcgt gcctgatcgg
     6901 ccgcgacgac gactgccgca tcaagatcga gcacccgcgc gtctcacggc ggcacgcgtc
     6961 ggtcgaatgc gcggccggca agttctttct gcacgaccac agcacgaacg gcacctacgt
     7021 cgccgagcgg cagaccgcac ccgtgctcgt ccatcgcgag ctgctgcagc tcaaggagtc
     7081 gggtgtcatc tcgctcggca tcgagccgca gctcaatgtc gatcactcga tcgtcttctc
     7141 gttcgagaag tgacgagccg gctcgtcgcg cttcaggccg aggcggccga cgcgacgaac
     7201 aaggcggcga acaaaacggc cgccgtcgca acgccgatca gaaacaacgt gtacgcgagc
     7261 tgcaagcagc ggtacttcgc cgtcagagct tcgcccatcg aatagagctg acgcgtgagg
     7321 ctgtcgtgca gcagtttgcg gtccttgccg agcgcgcgaa tcgcggcttg gaaatccgcg
     7381 agcggcatgc tcaggaaatg gccgaagaac agcagattcg cttggttgtt ccgcacctct
     7441 tcgacggtga ccggtgtgcg atccacgcgc gggcgcgcgg caatcacggc gaacgccagc
     7501 gacgacacgc agccggcaac cagcaccaac gccggcgcca gcgtccacga ccacgcgccc
     7561 gcccgcggcg cgacgaacgc gatgaggacg gagatgatca agccgttgat gctgatcaac
     7621 atgccggctt tctggtccgc aagctggctc agcgagatgt gattgcgata cgtgacgcgg
     7681 aacagcgtct cgaggccgcg tcgacgctcg gagcgcttct cttttttctc tttgtcgctc
     7741 ttgccgggct tgtcgggctt gtcgactttg gctggttgct cgttggcgtc ggcggcggag
     7801 gctagctcgg ttgacactgg cgagaccctc gttgtcgagc gccgggacta taccacgccg
     7861 ctccggagcg ccgaccgaca gcgccgacca gacagcgccg ctgtggtatg cctcgtccgc
     7921 gaggacgaca aggcggttgt cggattcgtg ccgatcgtta agccaccttg tcgaaagtcc
     7981 gaaactcgac aggcgccttg tcggtgccgg cgccttggcc gccacgggct cttgccgagc
     8041 cggcacgtct gcacctacga tcgatgcgcc ctgccggagc gaacaacaac aaggtcggct
     8101 cgatgcgctg caccgcctcg tcactgcttg cgatccgctc gcgcttgtac gctgcggcgg
     8161 cgctcgctgc cgccgtgacg tcgtcggcct cggccgatct gttcgcgacc gacgagccgc
     8221 tcgcattgac gctgtcggct ccgttcggcg ccgtgtttcg cgccaccacc gaggaccccg
     8281 agtaccaaga cgcgacgctc gcgtatcgca gcgccgacgg cagcgcagcg agcgtgccgc
     8341 tgcgtgtgcg cgtacgcggc aaatcgcgtg cggccgtctg cgacttcaag ccgctgttgc
     8401 tgaacttccg cacggccgat ctcgtcggca ccgcgttcga aggcgaggac cgcctgaagc
     8461 tcgtcacgca ttgcaaagcg agcgccagct acgacgaata cctgaagctc gagtacttga
     8521 gctaccgcgt gtggagcctc ctcagcgaga cggccctgcg cgcgcgcttg atcgaagtcg
     8581 cgtacgtcga ttcttcgcgc gcgcgcgacc tcggcacgcg tccaggcatc ctgctcgagg
     8641 acgaagggcg cttcgccgag cggcaagcgc tgacggtctt cgaaggcccg aaagtcgagc
     8701 gcgcaaagta cgacgccggc gcgctcgcgc tgctcgacgt gttccagtat ttcgtcggca
     8761 acacggactg gtccgcgctt gcaggcccgg ccggtcaaga gtgttgtcac aacgtcgtgc
     8821 cgtacgtgcg cgccgacggc gtcatggtgc ccgtgccgta cgacttcgat gcgacgggtc
     8881 tcgtcaacgc accgcacgcg ctgcccgacg agcgcttgcc gatccgcagc gtgcgccagc
     8941 gtctctaccg cggcgcgtgc cgcacacctg aggagctcgc gccgagcttc gagcgcttcc
     9001 aagcgcagcg cgcg
//
LOCUS       DATLEE010000448         3315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1249794, whole genome shotgun sequence.
ACCESSION   DATLEE010000448 DATLEE010000000
VERSION     DATLEE010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249794"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VM692_09655"
     CDS             complement(<1..386)
                     /locus_tag="VM692_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52475.1"
                     /translation="MRRHHAGLLGLGLAGFLAAPLHAQDAANWGLYSASKGATRYSPL
                     AQIDASNVNRLRVAWRHKQADPAILAANPDFALSNRYMVTPIYVDGLLYVPNGFGLTE
                     AIDPKTGVTRWTQKPLIDGPEGLPSL"
     gene            complement(410..910)
                     /locus_tag="VM692_09660"
     CDS             complement(410..910)
                     /locus_tag="VM692_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52476.1"
                     /translation="MLGLTHLGIIHTLLSLVAVAAGAAAFVRFGSISLRTRTGVVYVL
                     ATVLTCVTGFGIFKTGAFGPAHAVGVLTLVVLTFAVLVEKRTVLGSLSLALETVSYTT
                     TYFLHMIPAVNETTTRLPPSAPLAAGPNDPLVLGLVGLAFALYVIGAAFQLLRLKSIA
                     AAVRAS"
     gene            1048..1266
                     /locus_tag="VM692_09665"
     CDS             1048..1266
                     /locus_tag="VM692_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52477.1"
                     /translation="MAEDLAWVSIAERQPDDLQLVYARIKYGQPRKVTFYARPTPRWE
                     GANIVYDLGYFVEWAALDAQRRPPKRSE"
     gene            complement(1298..2323)
                     /locus_tag="VM692_09670"
     CDS             complement(1298..2323)
                     /locus_tag="VM692_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HUQ52478.1"
                     /translation="MRATILVGPGDVRIEQVPDAALVDETDAVVRVLKACICGSDLWP
                     YKEPSRNGTRMGHEAIGVVEDVGADVRTLKTGDVVVMPFAYSDGVCVFCQEGLHTACK
                     QGGFFGIGQVDGAQAEAVRVPLADGTLFALDVDEDDALLPSLLTLADVMGTGHHAAVT
                     ARVARGKRAAVVGDGAVGLCGVIAAKRLGAEQIILLGRHEDRIALARSFGATDIVSER
                     GEEAVERVLELTQGEGAHSVLECVGSEAAMVTAVSIARPGGAIGRVGVPHYEGIPALP
                     AFYANLNVAGGPAPVRAYMDELLPDILEGRIEPGRVFDRDVDLDGVPDGYRAMNERES
                     IKVMVSP"
     gene            complement(2405..3049)
                     /locus_tag="VM692_09675"
     CDS             complement(2405..3049)
                     /locus_tag="VM692_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUQ52479.1"
                     /translation="MTVTITAFEKSPDGGQGLARDTRVRWALEEVAQRYEVRLVSFRA
                     LKEPAHLHLHPFGQIPTYEEGELALFETGAIVLHLANTRAGLLPNDGNARARAIAWMF
                     AAVNTIEPPILELQNAKLLEGDKSWSKERWPLVVDRVRDRLKQLAARVGDAEWLDGAF
                     SAGDLMMVHVLQRLKPSGLLNEFPNLAAYVARGEARPAYKRAFEAQSAVYKANA"
     gene            complement(3097..>3315)
                     /locus_tag="VM692_09680"
     CDS             complement(3097..>3315)
                     /locus_tag="VM692_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUQ52480.1"
                     /translation="HDVKEIELDPRSEGFSAVISGHSHVPKSETREGVLYLNPGSAGP
                     RRFKLPVAVAKIHVSNDGVEAELITLTV"
BASE COUNT          585 a         1125 c         1053 g          552 t
ORIGIN      
        1 atcaacgacg gcagcccctc gggcccgtcg atcaacggct tctgcgtcca gcgcgtcaca
       61 cccgtcttcg ggtcgatggc ctccgtgagc ccgaagccgt tcggcacgta cagcaggccg
      121 tcgacgtaga tcggcgtcac catgtagcga ttcgacagcg cgaagtcggg gttcgccgcg
      181 aggatcgcag gatcggcctg cttgtggcgc cacgcgacac ggagccgatt gacgttcgac
      241 gcatcgattt gcgccagcgg cgagtagcgc gtcgcgcctt tgctcgcgga gtagagcccc
      301 cagttcgcgg cgtcctgcgc gtgcagcggc gcggccaaga accctgcaag acccaacccc
      361 agcaatccgg cgtgatgtcg gcgcattgcg tcgaaccccc ttgttttcgt tagctcgcgc
      421 gcacggccgc ggcgatcgac ttcaatcgca gcaactggaa cgcggcgccg atgacgtaca
      481 gagcgaacgc aaggccgacg agcccgagga ccagcggatc gttggggccg gcggccagcg
      541 gcgcactcgg cggcagacgc gtcgtcgtct cgttgacggc cggaatcatg tgcaggaagt
      601 aggtcgtcgt gtagctgacc gtctcgagcg ccagcgacag cgagcccaac accgtgcgtt
      661 tctcgacgag cacggcgaac gtcaacacga ccagcgtcag cacgccgacg gcgtgggccg
      721 gcccgaacgc gccggtcttg aagatgccga accccgtgac gcacgtgagc accgtcgcga
      781 gcacgtagac gactccggtg cgcgtgcgca acgagatcga gccgaagcgg acgaacgccg
      841 cggcaccggc cgccaccgcg acgaggctca gcaaggtgtg gatgattccg aggtgcgtca
      901 atccgagcat cgccgcctcc cgcggacgac ttggcgcagt gagtatctca gcgtcttcgt
      961 tccttagcca tccgcagcca agcagataat tcgtacgggc ccaggttaag agagtacgct
     1021 ggcaccgttc ccatcacgag aacaattgtg gctgaggacc tcgcctgggt ctcgatcgcg
     1081 gagcgccagc cagacgatct gcagctcgtg tacgcccgca tcaagtatgg gcagccacgc
     1141 aaggtgacct tttacgctcg accgacgcca cgatgggaag gcgccaacat cgtctacgac
     1201 ctcggctatt tcgtcgaatg ggcagcgctc gatgctcagc gtaggccgcc gaagcgctcc
     1261 gaatagccca attcgcgttt ctgcagggcg gccgctgcta tgggctaacc atgactttga
     1321 tcgactcgcg ttcattcatc gctcgatagc cgtcgggcac gccatccaaa tcgacgtcgc
     1381 gatcgaagac gcggccgggc tcgatgcgcc cttccaagat atccggcagc agctcgtcca
     1441 tgtaggcgcg gaccggcgcc ggaccgccgg ccacattgag gttcgcatag aaggccggca
     1501 gcgccggaat gccttcgtag tgcggcacgc cgacgcggcc gatcgcgcct ccggggcgcg
     1561 cgatgctcac ggctgtcacc atcgcggcct cgctgccaac gcactcgagc accgagtgag
     1621 cgccttcgcc ctgcgtgagc tcgagcacgc gctcgacggc ctcttcgccg cgctcgctga
     1681 cgatatcggt cgcgccgaac gaacgcgcca gcgcgattct atcttcgtgg cgtccgagca
     1741 aaatgatctg ctcggcgccg agccgcttcg ccgcgatgac gccgcataag ccgacggcgc
     1801 cgtcgccgac gacggcggcg cgcttgccgc gcgcgactct tgcagtcacc gcggcatgat
     1861 ggcccgttcc catcacgtcg gcgagggtca gcaaagacgg cagcagcgcg tcgtcctcgt
     1921 cgacgtcgag cgcgaacagc gtgccgtctg caagcggcac acggacggct tcggcctgtg
     1981 cgccgtcgac ctggccgata ccgaagaaac cgccctgctt gcatgccgtg tgcaaccctt
     2041 cttggcagaa cacgcagacg ccgtccgagt aagcgaacgg catgacgacg acgtcgccgg
     2101 tcttgagggt gcgcacgtcg gcgccgacgt cctcgacgac gccgatcgct tcatgcccca
     2161 tccgcgtgcc gttgcgcgaa ggttccttgt acggccaaag gtcgctgccg cagatgcacg
     2221 ccttcagcac acgcacgacg gcatcggtct catccacgag cgcggcatcc ggcacctgct
     2281 cgattcgaac gtcgccgggg cccacgagaa tagttgcacg catagctact ccttgctggc
     2341 cgccctctgc gctcgcgcag aagggctgat tgcaagcaag acacgtgccg gacccgaggg
     2401 ctggtcaggc gttcgccttg tagacagccg attgagcctc gaacgcgcgc ttgtaggcgg
     2461 gccgcgcctc gccgcgggcg acataggcgg ccaggttcgg gaattcgttg agaagaccgg
     2521 acggtttgag ccgctgcagc acgtgcacca tcatgagatc gccggcactg aatgcaccgt
     2581 cgagccactc ggcgtcgccc acgcgagcgg caagttgttt tagccggtcg cgcacgcgat
     2641 cgaccacgag gggccaccgc tccttggacc agctcttgtc tccctccagc agcttggcat
     2701 tctgcagctc gaggatcggc ggctcgatcg tgttcacggc ggcgaacatc caagcgatcg
     2761 cccgcgctcg cgcgttgccg tcgttcggaa gcaggcccgc tcgagtgttg gcgaggtgga
     2821 gcacgatcgc gcccgtctcg aacagcgcga gctcgccttc ctcataagtg ggaatctggc
     2881 cgaatggatg caggtgcagg tgcgcgggtt ccttcagcgc gcgaaacgac acgaggcgga
     2941 cctcatagcg ctgcgccact tcctcgagcg cccagcgcac gcgcgtatcg cgcgccagcc
     3001 cctggccgcc gtcgggtgat ttttcgaaag ccgtgatggt aacggtcatg gtgacgctcc
     3061 cttccgtggc aaacagcgct tcgatgtagg cctacgttac accgtcaacg tgatgagctc
     3121 cgcctcgaca ccgtcgttgc tcacgtgaat cttcgcgacg gcaaccggca acttgaaccg
     3181 cctcggcccg gcgctaccgg ggttcaggta cagcacgccc tctcgcgttt cactctttgg
     3241 cacgtgcgag tgacccgaga tcacggcgct gaagccttcg gagcgcggat cgagctcgat
     3301 ctctttgacg tcgtg
//
LOCUS       DATLEE010000449         3251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1249961, whole genome shotgun sequence.
ACCESSION   DATLEE010000449 DATLEE010000000
VERSION     DATLEE010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249961"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1062)
                     /locus_tag="VM692_09685"
     CDS             complement(133..1062)
                     /locus_tag="VM692_09685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUQ52481.1"
                     /translation="MQLILLSKDRGHLAHVRLTSGRVWVGIVVATLIVCAGAFYGGIT
                     AARVFGISNPQAQVSTWREELAQQQSIVDTTRRTLQQNLDALALRIGQMNAHVVRLDA
                     LGTRLTQMAGLKDGEFDFTSAPSLGGPEEPLAATEAMQINGVVGALDVLDEQLADRNR
                     QLTVLEDLLLNRKLRDEVRPEGRPVTQGYVSSQFGSRTDPFTGRRAFHKGVDFAGREG
                     AEVVAVASGVVIWSGERYGYGQLVEINHGNGYVTRYAHNVDNLVTVGDTVRRGQVIAR
                     MGDTGRATGPNLHFEVLLNDKPVDPLSYVQSAD"
     regulatory      complement(1160..1245)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1369..1596
                     /locus_tag="VM692_09690"
     CDS             1369..1596
                     /locus_tag="VM692_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52482.1"
                     /translation="MARILRESARRRTETARVRGLLPAEEAAHLVSATTNENGELVVV
                     MDTPSWAARVRYCVSALPSAAVKIRVLPRGG"
     gene            complement(1593..2501)
                     /gene="lpxC"
                     /locus_tag="VM692_09695"
     CDS             complement(1593..2501)
                     /gene="lpxC"
                     /locus_tag="VM692_09695"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HUQ52483.1"
                     /translation="MRQRTLKESIRATGIGLHTGEKVYMTLRPAPANSGIVFRRLDLP
                     EPVDVPADPLRVVETPLGTTLERDGAKVGTVEHLMSAMAGLGIDNAFVDLTAAEVPIM
                     DGSAAPFVFLLQSAGIEELNAPKRFIRVLKRIEVQDGDKWVRLDPHDGFRVNVEIDFE
                     HPALRKYRQAMTMEFSTQSFLKEISRARTFGFLKDLEGARARERALGANLDNAIVMDD
                     YRVINEDGLRFRDEFVRHKILDALGDLYLLGSSLIGEFSAFKCGHKLNNLLLRALLDA
                     PEAYESVEFDEGEHRSPVSYQLATSH"
     gene            complement(2655..>3251)
                     /locus_tag="VM692_09700"
     CDS             complement(2655..>3251)
                     /locus_tag="VM692_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399586.1"
                     /note="GTPase; similar structure to tubulin; forms
                     ring-shaped polymers at the site of cell division; other
                     proteins such as FtsA, ZipA, and ZapA, interact with and
                     regulate FtsZ function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HUQ52484.1"
                     /translation="DDVLRQAIQGISDLILVPGLINLDFADVKTIMSGMGVAMMGTGT
                     SEGENRAMQAAQKAVSSPLLEDINLSGANGVLVNVTAGVDLSIGEFQEVGDTIKQFAS
                     DDATVVIGTVIDPEMSDEIRVTVVATGLGQAVGMAERPMKVVRRAAPPPPKGREPNYT
                     ELEKPAVARKRAVGDGVTGNDSLEDLLDIPAFLRRQAD"
BASE COUNT          571 a         1047 c         1067 g          566 t
ORIGIN      
        1 ggctgccgaa cacgccgacc atgaagttcg agacggtgct gagcacggcg gggagtcctt
       61 aggcggggca gtaaagcggc ctattctaag gacctcggcc ggttttgggc accggtcgaa
      121 cgttacggag ggttaatccg ccgactggac gtagctcagc ggatcgacgg gcttgtcgtt
      181 caggagcacc tcgaagtgca ggttcggccc cgtcgcccgg cccgtatcgc ccatgcgcgc
      241 gatcacttgg ccgcggcgca cggtatcgcc gacggtgacg aggttgtcca cgttgtgcgc
      301 ataccgagtc acgtaaccgt tgccgtgatt gatctcgacg agctgaccgt agccgtaacg
      361 ctcgccggac cagatcacga cccccgacgc gacggccacg acctcggcgc cttcgcgacc
      421 cgcgaagtcg acgcccttgt ggaaagcgcg gcggcccgtg aacgggtccg tgcggctacc
      481 gaactgcgac gacacgtacc cttgagtgac gggccggccc tcgggacgaa cctcgtcgcg
      541 cagcttgcga ttcaacagca ggtcttcgag gaccgtcagc tgccgattgc ggtccgcgag
      601 ctgctcgtcg agcacgtcga gcgcgccgac gaccccgttg atctgcatgg cctccgtcgc
      661 ggcgagcggc tcttcgggtc cgccgagcga cggagccgac gtgaaatcga attcgccgtc
      721 cttgaggccg gccatctgcg tgaggcgcgt gccgagtgcg tcgaggcgga cgacatgggc
      781 gttcatctgc ccgatgcgga gcgcgagagc gtcgaggttt tgttgcagcg tccgccgcgt
      841 ggtgtcgacg atggattgct gctgcgcgag ctcctcgcgc cacgtggaaa cttgagcttg
      901 cggattgctg atgccgaaca cgcgggccgc ggtgatgccg ccgtagaagg ccccggcgca
      961 aacgatcagg gtggcgacga cgatgccgac ccacacgcga ccggaagtca accggacatg
     1021 ggccaaatgc ccccggtcct ttgacaggag tatcaactgc atgcggtgcc ccagctcctg
     1081 gcttctaatt ctttgatcca gcgccaaggc aggcgccggc atgcaccgcg agtatagagg
     1141 gacgatttgt caaaagtcac tcggcaaccc cgctatcatt ggagaaactc cggtagatcg
     1201 gtggctttgc gtcccgtcct ttcgaacggt ttgccttgat tcgagcaagg gccaggatgc
     1261 cggtaaaacg cgcaccggcc aagtgaattt cccccaaagg tagacgttgg tctcataacg
     1321 atgagcgatg agccgcggca tttgtcagaa ctactggaac aaggtccgat ggcgcggatc
     1381 ttgcgagaat ccgcgcgtcg ccgaacggag acagctcgcg ttcgcggctt gttgccggcc
     1441 gaagaagccg cgcatctcgt cagcgcgacg accaacgaga acggcgagct cgtggtcgtg
     1501 atggacacgc cgagctgggc cgcacgcgtg cgctattgcg tgagcgcgct gccctccgcc
     1561 gcagtcaaga tccgcgtgtt gccgcgcggc ggctagtggc tcgtcgcgag ctggtaggag
     1621 accggcgagc gatgctcgcc ttcgtcgaac tcgacggact cgtaagcctc gggcgcgtcg
     1681 agcaaggcgc gcagcagcag gttgttgagc ttgtggccgc acttgaaggc cgagaactcg
     1741 ccgatcagcg acgagccgag caggtatagg tcgccgagcg cgtcgagaat tttgtgccgc
     1801 acgaactcgt cgcggaaccg caggccgtcc tcattgatta cgcggtagtc gtccatgacg
     1861 atcgcgttgt cgagattcgc gccgagcgca cgctcacgcg cgcgggcgcc ttcgagatcc
     1921 ttcagaaagc cgaacgtgcg cgcgcgactg atctctttca gaaacgactg cgtcgagaac
     1981 tccatggtca tggcttggcg atacttgcgc agcgccggat gctcgaaatc gatttcgacg
     2041 ttgacgcgaa aaccgtcatg cgggtcgagc cgcacccact tgtcgccgtc ttggacctcg
     2101 atgcgcttca gcacccggat gaagcgcttc ggcgcattga gctcctcgat gccggcggac
     2161 tgcagcaaga acacgaacgg cgccgcgctg ccgtccatga tcggtacctc ggccgccgtc
     2221 aggtcgacga acgcgttgtc gatgccgagc ccggccatcg cggacatcag atgctcgacc
     2281 gtgccgacct tcgcgccgtc gcgctcgagc gtcgtgccga gcggcgtctc gacgacgcgc
     2341 aacggatcgg cgggcacgtc gacgggctcc ggcagatcga ggcgccgaaa cacgatgccg
     2401 gaattggccg gcgccggccg caaggtcata tagaccttct cgccggtgtg caggccgatg
     2461 ccggtggcgc gaatcgattc cttgagagtg cgttgtcgca cgctagccgt tccctatggc
     2521 ggtttgctcg ccaatgccca cgaggcgtcg ccggattgcg acacccaaac ggtagacccg
     2581 tcgcggggcc gacgaaccta ccatagcggc atttcgagca gcaaggaaac gccgcttgcg
     2641 gaggcgagcg ggcatcagtc cgcttgccgc cgcaagaacg ccggaatatc cagcaagtcc
     2701 tcgaggctgt cgttgcccgt aacgccgtcg ccgacggcgc gcttgcgggc cacggccggc
     2761 ttctcgagct ccgtgtagtt cggctcgcgg cccttcggcg gcgggggcgc ggcgcggcgc
     2821 acaaccttca tcgggcgctc ggccatgccg acggcctggc cgagaccggt cgcaacgacc
     2881 gtcacgcgga tctcgtcgct catctcgggg tcgatcacgg tgccgatgac gacggtcgcg
     2941 tcgtccgacg cgaattgctt gatcgtgtcg ccgacctctt ggaactcgcc gatcgacaga
     3001 tcgacgccgg ccgtcacgtt gacgagcacg ccgttcgcgc cggagagatt gatgtcctcg
     3061 agcaacgggc tcgagacggc cttctgcgcc gcctgcatcg cacggttctc gccttccgac
     3121 gtgccggtcc ccatcatcgc cacgcccatc cctgacatga tggtcttgac gtccgcaaag
     3181 tcgaggttga tgaggccggg cacgagaatc aggtcggaaa tcccctgtat cgcctggcgc
     3241 aatacgtcgt c
//
LOCUS       DATLEE010000450         4096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1253815, whole genome shotgun sequence.
ACCESSION   DATLEE010000450 DATLEE010000000
VERSION     DATLEE010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253815"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2323)
                     /locus_tag="VM692_09705"
     CDS             complement(<1..2323)
                     /locus_tag="VM692_09705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HUQ52485.1"
                     /translation="MNPPNGAYAELHCLSNFSFLRGASHPGELVQAAAELGYAGLAVT
                     DECSVAGVVRAHTAAKDLPLKLLVGSELTCHDGLKVVALAESRNAYAALCGLISRARR
                     AAPKGEYRLARDDIAECLGAHGLLLWIPSGSRDVDLQAGRWLQDRFAGRLWLTVELLN
                     AGNDRRLQLEARALGRELGIPLVAAGNVHMHCRERRMLQDTLTAIRRKVPIDELGFEL
                     HSNAERSLRPIDELVRRYPADLLRETLAILERVNFSLTELRYEYPNELIPAGENPSSY
                     LRALTERGCRWRWPAGESPKVRKLIEHELQLIGELRYEAYFLTVHDIVSYARSIGILC
                     QGRGSAANSAVCFCLGITEVDPDRMQTLVERFISKERNEPPDIDVDFEHERREEVIQY
                     IYRKYSRERAALAATVITYRGRSAIRDVGKALGVGELQVSALAGSLQWWESGVVDEQR
                     IREAGLDPKSPKVWRWIRLAESLLGFPRHLSQHVGGFVIAERPVHELVPIENAAMPER
                     TVIQWDKDDLEELGLMKVDVLGLGMLSAVRRSFELIEKFSGTRHTLATVPSEDEGVYE
                     MICRADTMGVFQIESRAQMAMLPRLKPKCYYDLVIEVAIVRPGPIQGDMVHPYLRRRE
                     GSEPVTYPSAAVEQVLQRTLGVPIFQEQVMQLAIVAAGFTPGESDQLRRAMAAWRRNG
                     HLEKFEQRLISGMIERNYSEEFAQRVFSQIKGFGEYGFPESHAASFALLVYVSSWLKC
                     YEPAAFCCALLNSQPMGFYAPQQLVRSARDHGVE"
     gene            complement(2347..3849)
                     /locus_tag="VM692_09710"
     CDS             complement(2347..3849)
                     /locus_tag="VM692_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HUQ52486.1"
                     /translation="MPRVVKSLALFPPAPVEAPRFESRPAPSRSQLWIAVCLPSLALE
                     CLSAPDRLARQVVVEAEQGQLHVVASSQAAVDVGIVAGMKLSAAMALEATLQVFERAP
                     LLERASLESLASWAHTLTSQVSLEAPESLLLEVAGSFKLFGSFEAIKAKLSEELARRY
                     RDYRVCAAPTATGALWLARAASDDALQQPQLGGRLAALPLAVTRWPPAVQALLQDLGL
                     RTVGDSARLPRDGFARRVGRRYLVELDRAFGRAHDLRAEFKAPTTWKARAELSEESVD
                     SAVFLEALEQLLDALAVELKRRQVQIGRLEIAFEHLHRPPTVESFDLREPTHDRDRLL
                     DLIEDRLERSVLPVPAVALRIESDAFRPLELEEAGLFETKPLEQREQVLLERLQERFG
                     LGRVYGLRTVAEHRPEKAWDKNLESDGRRVAPPWQHREARPLWLLPAPVEASRDSLEL
                     VSGPERIESGWWDEHDVTRDYYTAKNDQGQTLWVFRDHRTRGWFLHGLFG"
     gene            complement(3859..>4096)
                     /locus_tag="VM692_09715"
     CDS             complement(3859..>4096)
                     /locus_tag="VM692_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52487.1"
                     /translation="ILRRLQLAAEDQGCWVVLFRPVSASQQRSPAALRIRLSQAQAAT
                     RVQIIKCRGGRPNVVELASRTGDAGGLFAEATSR"
BASE COUNT          758 a         1358 c         1258 g          722 t
ORIGIN      
        1 cctcgacgcc atgatcgcgc gccgaacgca cgagctgctg cggcgcatag aaacccatcg
       61 gctgactatt caatagtgcg cagcagaatg ccgcgggctc gtagcacttg agccaggacg
      121 agacgtagac gagcaaagca aagctcgccg catgactttc ggggaagccg tattcgccga
      181 agcctttgat ctgactgaac acccgctgag cgaactcctc gctgtagttg cgctcgatca
      241 tgccgctaat caaccgctgc tcgaactttt cgagatgacc gttgcggcgc catgcggcca
      301 tggcgcggcg cagctgatcc gactcgccgg gcgtgaagcc ggccgcaacg atcgcgagct
      361 gcatgacctg ctcttgaaaa atcggcacgc cgagcgtgcg ctgcagcact tgctcgaccg
      421 ccgcgctcgg ataggtaacg ggctcgctgc cttctcgtcg gcgaagatac ggatgcacca
      481 tgtcgccttg aatcggcccc gggcgaacga tggcgacttc gatcacgagg tcgtagtaac
      541 acttgggttt aagtcgcggc agcatcgcca tctgcgcccg cgactcgatc tgaaacacgc
      601 ccatcgtgtc ggcgcggcag atcatctcgt agacaccttc gtcctcggag ggaacggtcg
      661 cgagcgtgtg gcgggtaccc gaaaactttt cgatgagctc gaagcttcgg cgcacggcgc
      721 tcaacatgcc gaggcccaag acgtcgactt tcatgagccc gagctcctcg aggtcgtctt
      781 tgtcccactg gatgacggtg cgctcgggca tcgcggcatt ttcgatggga acgagctcgt
      841 ggaccggccg ctcggcgatg acgaagccgc ccacgtgctg cgagagatgg cgagggaaac
      901 caagcagcga ctcggccaag cgaatccatc gccacacttt cgggctcttg gggtcgagcc
      961 ctgcctcgcg aatccgctgc tcgtcgacga cgccgctttc ccaccattgc agcgagcccg
     1021 cgagcgcgct gacttgcagc tcgccgactc caagcgcctt gccgacgtcg cggatggcgc
     1081 tgcggccgcg ataggtaatg accgttgccg ccaacgccgc ccgctcgcgc gaatatttgc
     1141 ggtagatata ttgaatgacc tcttcgcgcc gctcgtgctc gaaatccacg tcgatgtccg
     1201 gcggctcgtt gcgctctttc gagatgaacc gctcgacgag cgtctgcatg cgatcgggat
     1261 cgacctccgt aatacctaag cagaagcaca cggcggaatt cgcggccgag cccctgccct
     1321 ggcacaagat gccgatgctg cgggcgtagc tcacgatgtc gtgcacggtc aagaagtacg
     1381 cctcgtagcg cagctcgccg atcagctgaa gctcgtgctc gatgagcttg cggactttgg
     1441 gcgattcccc tgccggccat cgccaccgac aaccgcgctc ggtcagcgca cgcagatagc
     1501 tcgatggatt ttcgccggcc ggaatgagct cgttcggata ctcgtaccgc agctccgtca
     1561 gcgaaaaatt cacgcgctcg agtattgcga gcgtctcgcg cagtaagtcg gcgggataac
     1621 gccgcacgag ctcgtcgata ggccgcagcg agcgctcggc gttgctgtgc agctcgaagc
     1681 cgagctcgtc gatcggcacc ttgcgtcgta tggccgtcag cgtgtcttgc agcatgcggc
     1741 gctcgcggca atgcatgtgc acattgccgg ccgcgaccag cgggattccg agctcgcggc
     1801 cgagagctcg cgcctcgagc tgcaaacgcc ggtcgttgcc ggcgttcagg agctcgacgg
     1861 tcaaccagag acggccggcg aaacgatctt gcagccagcg ccctgcctgc aaatccacat
     1921 cgcgcgagcc gctcgggatc cagagcagca gaccgtgcgc tcccaagcac tccgcgatgt
     1981 cgtcacgcgc gagccggtac tcgcctttgg gagccgcgcg ccgtgccctg ctgatgagcc
     2041 cgcacaacgc cgcatacgcg ttccggctct cggcgagcgc tacgaccttg agcccgtcat
     2101 gacatgtgag ctcgctgccg actagcagct tcaacggcaa atctttcgct gccgtatgcg
     2161 cccgcacgac tccggccacc gagcattcgt ctgtcacagc cagtccggca tacccgagct
     2221 cggccgccgc ttgcacgagc tcgccgggat gcgatgctcc gcgcaagaac gaaaagttgc
     2281 tgagacaatg aagctcggcg taggcgccgt tggggggatt catgacggct tcaatgactt
     2341 ctgggctcaa ccgaataacc cgtgcaagaa ccagccgcga gtgcggtgat cgcgaaacac
     2401 ccacagcgtc tggccctggt cgtttttggc cgtgtagtag tcgcgggtga cgtcgtgctc
     2461 gtcccaccaa ccgctttcga tgcgctcggg gcccgacacg agctcgagtg agtcgcggct
     2521 tgcctcgact ggagccggca acaaccacaa cggtcgcgct tctctgtgct gccacggtgg
     2581 ggccacgcgg cggccgtcgc tctcgagatt tttgtcccag gccttttccg gacggtgctc
     2641 cgcaaccgtg cgtaacccgt agaccctgcc caagccgaat cgctcttgca gccgctcgag
     2701 caatacctgc tctcgctgct cgagaggttt cgtttcgaac aaacccgcct cttcgagctc
     2761 gagcggccga aaagcatcgc tctcgattcg cagcgcgacg gccggcaccg gcaagacgct
     2821 acgctccaat cgatcctcga tgagatcgag caatcgatct cgatcgtggg tgggctcgcg
     2881 caaatcgaag ctttcgaccg tgggcggccg gtgcagatgc tcgaacgcga tctcgagcct
     2941 gccgatttgc acttgccgcc gcttgagctc gacagcgagc gcgtcgagca gctgctcgag
     3001 cgcttcgaga aagacggcgc tgtcgacgct ttcctcgctg agctccgctc tcgctttcca
     3061 agtcgtcggc gccttgaact cggcacgcaa atcgtgcgcg cggccgaacg ctcgatcgag
     3121 ctcgacgaga taacgacgcc ccacgcgacg agcgaagccg tctctcggca ggcgcgcaga
     3181 atcgccgacg gttcgtagcc ccaaatcctg caacaatgcc tgcacggccg gcggccaccg
     3241 cgtcaccgcc aatggcagcg ctgctagacg tcccccgagt tgtggctgct gcaaagcgtc
     3301 gtcgctcgcg gctcgcgcca gccacaaagc tccagtcgcc gtcggcgccg cgcacactcg
     3361 ataatcgcga tagcgccgag cgagctcctc gctgagcttg gctttgatgg cttcgaaaga
     3421 gccgaacaac ttgaagctac ccgccacctc gagcaatagg ctttcgggcg cttcgagact
     3481 gacctgcgaa gtcagcgtgt gcgcccagct cgccaaggat tcgaggctgg cgcgctcgag
     3541 aagcggcgcg cgctcgaaca cttgcagagt agcttcgagc gccatggccg cgctcaattt
     3601 catcccggcg acgattccta cgtcgacggc ggcctggctc gaagcaacga cgtgcagttg
     3661 gccttgctcg gcctcgacga ctacttgacg cgcgagtcga tcaggagccg aaaggcattc
     3721 gagcgccaag ctcggcaggc agaccgcgat ccaaagctgc gaccggctcg gcgccggacg
     3781 gctttcgaag cgcggagctt cgacgggagc cggcgggaat aaggcgagag atttgacgac
     3841 gcgcggcata gaagcggctc accggctcgt ggcctccgcg aagaggccgc cggcgtcgcc
     3901 cgtgcgactg gccagctcta cgacattggg tcgaccgccg cggcatttga tgatttgcac
     3961 gcgcgtcgcc gcttgcgctt gcgacaagcg aattcgtaaa gcggcaggcg atcgctgctg
     4021 actcgcgctc acgggtcgaa acagcacgac ccagcaacct tgatcttcgg cggccaattg
     4081 caggcgccgc aaaatg
//
LOCUS       DATLEE010000451         4937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1997824, whole genome shotgun sequence.
ACCESSION   DATLEE010000451 DATLEE010000000
VERSION     DATLEE010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997824"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VM692_09720"
     CDS             <1..930
                     /locus_tag="VM692_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ52488.1"
                     /translation="ERHQVVWGLGRRHHDYDIVNTPGLAFAPPSRTLELTNVFAQDVI
                     ALGTRFELTAGVKFEENSYSGWSTLPDLRLSWAPNEHALVWGAASRAIRAPTPLDVDV
                     RENVNGALFLTGNADFRPEEVDAYEIGYRSQPNASVSWFVSTFYNDHDDLRTIEPAVG
                     TFTPLRWDNLLEGHTYGVEAWANIQVKPWWRLSPGYRSVTKRLRFSEGASQLLGTAQA
                     GNDPRYSVQLKSAMAFGRFSVDAMLRHIGELPSPANPDYTELSARVSWRASDKLELAL
                     TGFNLLDERHSEYAVPTAREIPRTFYAEARWTF"
     gene            989..1375
                     /locus_tag="VM692_09725"
     CDS             989..1375
                     /locus_tag="VM692_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52489.1"
                     /translation="MRLDTRFKSATVLVTACALAASIAWAQDLTGKWTATAVANNAAV
                     TLDLKVAGNVVTGAILNPNAGPAEIKDGRLDGDKISFHVVRTRDNGTTDKILWQGTVH
                     GAEIHFMRSIEGATTPAMEIVAKRAQ"
     gene            complement(1476..2426)
                     /locus_tag="VM692_09730"
     CDS             complement(1476..2426)
                     /locus_tag="VM692_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52490.1"
                     /translation="MSAIVEPGNAFDDRSGFAGTANPSTVASRSAVSWGAVFAGAAGA
                     AALSLILLLLGTGLGLSSVSPWASEGVDASTFGISTILWITFMQLAASAMGGYLAGRL
                     RTKWVAVHTDEVYFRDTAHGFLAWAVATLGTAALLSSVIGAILGAGVQAGATAVGGAA
                     SAAVSGATVAGAQADPKQNEADGPMGYFVDSLFRASPEERRGDVPPEQTAAATAEVTR
                     IFANAIRTGTLPDADVRYVGQVVAQRTDMSQEQAEQRVRDVFADVQKSLQDAEAAARE
                     AADKAREASAYAALWLFISLLAGAFIASWVATYGGRQRDL"
     gene            complement(2497..2679)
                     /locus_tag="VM692_09735"
     CDS             complement(2497..2679)
                     /locus_tag="VM692_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52491.1"
                     /translation="MLTEALHAMMFGDDLWLFAGAVLGAVIAAAIPWAAMTIVRLVGA
                     LPRILRLRQVGKPDRP"
     gene            complement(2669..2914)
                     /locus_tag="VM692_09740"
     CDS             complement(2669..2914)
                     /locus_tag="VM692_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52492.1"
                     /translation="MQQRSAETWLTDEIEAAIQAYCATLTEMASDAAQGRAVSAEQLE
                     RAAEAGLRLENASTLFELSAWRRRQRPTQLPAPRRAH"
     gene            complement(2992..4344)
                     /locus_tag="VM692_09745"
     CDS             complement(2992..4344)
                     /locus_tag="VM692_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ52493.1"
                     /translation="MPNELDVNQFLDTRRVGRTHMLVVALLILTMMVDGYDIFVMGIV
                     APYLARDLAIPPESLTSVLVITQLGLLLGNFVVGPVADRYGRRFTMLWSLLVFGLLTL
                     ATPLASTVSELFVLRFIAGLFLSGVIPNAIALVSEIMPSHVRATFVSITFAGYTLGTS
                     VIGAPVVKWLVPLGWEYAFVVGGVLPLVLTVLLFFALPESLRFLASRRPHDPRIPRQL
                     KRMDATLALAGNETFTARGDARAASKAPIAALFRDGRWVITLLLWTGFHMAFIVSNLL
                     GSWRNVVLNQNGLTTDQIATIMFLPGIAGVIGTLTSGFVMERLGPTRVLPVYLGAASL
                     STAAVAFFDLTSIWTLLAFIVTGYFSNGGLSGINALASLTYPSQMRATGVSFAHSAGR
                     AGAIVGPFIGGAMIAAQMSAPAVFLVTAIPQMCAALAIFVLWRVQAQRAAGAIALVPR
                     "
     gene            complement(4348..>4937)
                     /locus_tag="VM692_09750"
     CDS             complement(4348..>4937)
                     /locus_tag="VM692_09750"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52494.1"
                     /translation="DSKLWATSRQALASVAAFRELELTPAQADTIERIVVAVPTAYRA
                     MIDRAAPPTQRIESMIAVQHQFALAVFAPQVLDDALRRAVSTPPAFAALMAKIEVRAD
                     EALDAHFPRKWGGRVTVRFGSGEERTREVLDPDGSAARPLDLAAIARKYARVFAASDV
                     REPDGWLRATRERCERLGRSEADASIVAELLASIR"
BASE COUNT          897 a         1633 c         1647 g          760 t
ORIGIN      
        1 gaacggcatc aagtggtctg gggactcggc aggcgacacc acgactacga tatcgtcaat
       61 acgccggggc tcgcgttcgc gccgccgagt cgcacgctcg agctgacgaa cgtctttgcg
      121 caagacgtca tcgcgctcgg cacgcgattc gagctgacgg cgggcgtcaa gttcgaagaa
      181 aattcgtatt cggggtggtc gacgttgccg gacttgcgcc tgtcgtgggc gccgaacgag
      241 cacgcgctcg tttggggcgc cgcgtcacgc gcgatacgtg cgccaacgcc gctcgatgtg
      301 gacgtccgag agaacgtcaa cggcgcattg tttctcaccg gcaatgccga tttccgcccc
      361 gaagaagtcg atgcctacga gatcggctat cgcagccagc cgaacgcgtc ggtgtcgtgg
      421 ttcgtcagca cgttctacaa cgaccacgac gatctacgaa cgatcgagcc cgccgtgggc
      481 acgttcacgc cgttgcggtg ggacaatctg ctggaaggcc acacctatgg cgtcgaggct
      541 tgggcgaaca tccaagtgaa gccgtggtgg cgcttgtcgc cgggctatcg ctcggtgacg
      601 aagcggctgc gcttcagcga gggcgcttcg cagctgctcg gcacggcgca ggccggcaac
      661 gacccgcgct acagcgtcca gctcaagtcg gcgatggcat tcgggcgctt cagtgtcgat
      721 gcgatgctgc gccacatagg cgagctgccc tcgcctgcga atcccgacta cacggagctc
      781 agcgcgcgcg tgtcttggcg cgcctccgac aagctcgagc tcgcgctcac gggcttcaat
      841 cttctcgatg agcggcacag cgaatatgct gtgccgaccg cgcgcgagat tccgcggacg
      901 ttctatgcgg aagctcgatg gacgttttga ccgctgacgc ctacgcttgc gcgatcagcg
      961 ccgcgtggcg agacggagtg aagagagcat gcgattagac acacgattca agtcagcgac
     1021 ggtgctcgtg acggcgtgcg cgctcgcggc gagcatcgcg tgggcgcaag acctcacggg
     1081 caagtggacg gcgacggcgg tcgccaacaa cgccgccgtc acgctcgatc tcaaggtggc
     1141 cggcaacgtc gtcacgggcg cgatcttgaa cccgaacgcc ggtccggcgg agatcaagga
     1201 cggcaggctc gacggcgaca agatctcatt ccacgtcgtg cgcacgagag ataacgggac
     1261 gacggacaag attctttggc aaggcaccgt ccacggcgcg gagattcact tcatgcgcag
     1321 catcgaaggc gcgacgactc ccgcaatgga aatcgtcgcg aagcgtgcgc aatgacagct
     1381 cagtgaaaca gcgccagcaa gatgacgatc gggagcggca ctccgagtat caacaacaag
     1441 atcgagcgca tggctgcctc ctttcggtcg agtggttaaa gatcgcgttg gcgcccgcca
     1501 taggtcgcca cccagctcgc gatgaacgcc ccggccagca gcgagatgaa cagccataac
     1561 gccgcatagg ccgacgcctc tcgcgccttg tcagcggctt cgcgcgccgc ggcctcggcg
     1621 tcctgcaagc ttttctgcac gtcggcaaac acgtcgcgca cgcgctgctc ggcttgctcc
     1681 tgcgacatgt cggttctctg cgcaaccact tgcccaacgt aacgaacgtc cgcatcgggc
     1741 aaagtgcccg tacgaatggc attggcgaag attcgcgtca cctcggcggt cgcagcggcc
     1801 gtctgctccg gcggcacgtc gcctcttcgc tcctcggggc tcgctctgaa cagcgagtcg
     1861 acgaagtaac ccatcgggcc gtcggcttcg ttctgtttcg ggtcggcctg cgctccggcg
     1921 accgtcgcac cgctgacagc ggccgatgca gcgccaccga ccgcggtggc accggcctga
     1981 actccggcgc cgaggatcgc gccgatgacg gacgacagca gcgcagccgt gccgagcgtc
     2041 gcgacggccc acgccaagaa cccgtgcgcg gtatcgcgga agtacacctc gtcggtgtgc
     2101 acggcgaccc atttggtccg cagtcgcccg gcgagataac cgcccatcgc ggaggccgcc
     2161 aattgcatga aagtgatcca cagaatggtg gagatcccga acgtgctcgc gtcgacgcct
     2221 tcgctggccc acggcgagac cgacgacaat ccgagtccgg tcccgagcaa cagcaggatc
     2281 agagacaggg ccgcggcccc tgccgcaccc gcaaaaacgg cgccccatga aacggcgctg
     2341 cgcgaggcta cggtggacgg gttggctgtg ccggcgaagc cgctacggtc gtcgaatgca
     2401 ttaccgggct ctacgatggc gctcatgagg ctgtcctttc gctgctttcg caattacgtt
     2461 gtaactgcaa gaaacgagcc agccagcgcg cagcggtcaa gggcgatcgg gcttgccgac
     2521 ctggcgcagc cgcaggatgc gtggcagcgc gccgacgagc cggacgatcg tcatcgcggc
     2581 ccaggggatc gcggcggcga tgacggcacc gagcacggcg cccgcgaata gccaaagatc
     2641 gtctccgaac atcatcgcgt gcagcgcttc agtgagcacg gcgcggtgcg ggcagctgcg
     2701 taggccgctg gcgccgtcgc caggcggaga gctcgaacag cgtgctggca ttctcgagtc
     2761 gcagaccggc ttcggccgct cgctcgagct gctcagccga tacggctcgg ccttgtgcgg
     2821 cgtcgctcgc catctcggtc aacgtcgcgc aatacgcttg gatggccgct tcgatctcgt
     2881 ccgtgagcca cgtttccgcc gatcgctgct gcatacgccg ctccttggct tgctttctct
     2941 tctgcgcttt ttcttgtgca tctttcgggc caggagggcc cgtgctgaca gttatcgagg
     3001 aacgagcgcg atcgcgccgg cagcgcgttg ggcctgcacg cgccatagga cgaagatcgc
     3061 gagcgcggcg cacatctgcg gaatcgcggt cacgaggaac acggccggcg cgctcatttg
     3121 cgcggcgatc atcgcgccgc caatgaacgg ccccacgatc gcgccggcgc ggcccgcgga
     3181 atgcgcaaag ctcacacccg ttgcgcgcat ttgcgacgga tacgtcagcg acgcgagcgc
     3241 gttgatgccg ctcaggccgc cgttcgagaa gtagcccgtg acgatgaagg ccagcagcgt
     3301 ccagatcgac gtgagatcga agaacgccac ggccgccgtc gacagcgacg ccgcgccgag
     3361 ataaaccggc agcacgcgcg tcggaccgag acgctccatg acgaagccgc tcgtcagtgt
     3421 gccgatcacg ccggcgatgc cgggcaggaa catgatcgtc gcgatctggt cggtcgtcag
     3481 gccgttctga ttcagcacga cgttgcgcca cgacccaagc aggttgctga cgatgaacgc
     3541 catatgaaag ccggtccaca acagcagcgt gatgacccag cggccgtcgc ggaacagcgc
     3601 cgcgatcggc gccttgctcg ccgcgcgagc gtcgcctcgc gccgtgaacg tctcgttgcc
     3661 cgcgagcgcg agcgtcgcgt ccatgcgctt gagttgcctc gggatgcgcg gatcgtgcgg
     3721 cctgcggctc gcgaggaacc gcagcgactc cggcagcgcg aagaacagga ggacggtcag
     3781 cacgagcggc agcacgccgc cgacgacgaa cgcgtactcc caccccaggg gcacgagcca
     3841 cttgaccaca ggtgcaccga tcacggacgt gccgagcgtg tagcccgcga acgtgatcga
     3901 cacgaacgtc gcgcgcacgt gcgacggcat gatctccgac acgagcgcga tcgcgttcgg
     3961 aatgacgccg ctcaggaaca gacccgcgat gaagcgcagg acgaagagct cgctgaccgt
     4021 cgaggctaac ggcgtcgcga gcgtcagcag gccgaagacc agcagagacc aaagcatcgt
     4081 gaagcgtcgg ccgtagcggt cggcgacggg gccgacgacg aagttgccca gcagcaagcc
     4141 gagctgcgtg atgacgagca cgctcgtcag cgactcgggt ggaatcgcga ggtcgcgcgc
     4201 caggtacggc gcgacaatgc ccatgacgaa gatgtcgtag ccgtcgacca tcatcgtgag
     4261 gatcagcagc gccaccacga gcatgtgcgt gcgcccgacg cggcgcgtgt cgaggaactg
     4321 attgacgtcg agctcgttag gcatggctta acggatcgac gccaggagct ccgccacgat
     4381 gctcgcgtcg gcttcgctgc gcccaagccg ctcgcagcgt tcgcgcgttg cgcgcagcca
     4441 gccgtcgggc tcgcgcacat cgctcgccgc gaagacgcgc gcgtattttc gcgcgatcgc
     4501 agcaaggtcg agcgggcgcg cggcgctgcc gtcggggtcg agcacctcgc gcgtgcgctc
     4561 ctcgccggag ccgaagcgca cggtcacgcg ccctccccat ttgcgcggga agtgggcatc
     4621 gagcgcctcg tcggcgcgga cctcgatctt ggccatcaga gccgcgaagg ccggcggcgt
     4681 cgacacggcg cgccgcagcg cgtcgtcgag cacttgtggc gcgaagaccg cgagtgcgaa
     4741 ttggtgctgg acggcgatca tcgactcgat gcgctgcgtc ggcggcgccg cgcggtcgat
     4801 catcgcgcgg taggcagtcg gcacggcgac gacgattcgc tcgatcgtat cggcctgcgc
     4861 cggagtgagc tcgagctcgc ggaatgccgc aacgctggcg agcgcctgcc gcgacgtcgc
     4921 ccagagcttg ctgtcga
//
LOCUS       DATLEE010000452         3833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2000711, whole genome shotgun sequence.
ACCESSION   DATLEE010000452 DATLEE010000000
VERSION     DATLEE010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000711"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VM692_09755"
     CDS             <1..611
                     /locus_tag="VM692_09755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ52495.1"
                     /translation="ASSREHEALARLRKATRSVPNSVMQIGPDQGQLMALLVRLIGAR
                     RCIEVGTYTGYSALAVALALPPQGQLITCDVSAEWTNVGRPFWREAGVEARIDLRLKP
                     ALETLDELLVKGEAGRFDFAFIDADKSNYVAYYERLLQLVRQNGLIAVDNTLALAGAP
                     IFAQESESAKAMRVFNELVHDDERVDIAMLTVGEGLTLLRVR"
     gene            877..1287
                     /locus_tag="VM692_09760"
     CDS             877..1287
                     /locus_tag="VM692_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HUQ52496.1"
                     /translation="MAHCNQRTCRETTKSVFGSQTAKEAFHDNFSSFRSATFRGSVAR
                     WSTASIGAPRVGEIATTTVKVADLDISTASGAETLYKRIVTAARVVCFDTDVKAMHAC
                     RARAVADAVARVGSPLLSAAHRSTIERAEEVVRR"
     gene            complement(1358..2725)
                     /locus_tag="VM692_09765"
     CDS             complement(1358..2725)
                     /locus_tag="VM692_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HUQ52497.1"
                     /translation="MAIQFSPEEILETIRMVQMDNLDIRTITMGISLRNCADASLEAA
                     AKKAYEKICRAAARLVAVGEEIEREYGIPIINKRVSVTPVSLVAEASASDDYVLFAEY
                     LERAAAEVGVNFLGGFSALVHKGTTPGDAALLASIPEALARTERVCASINVATTRAGI
                     NMDAVARMGEIIVATAKRTADRDGIGCAKLVVFCNAVEDNPFMAGAFHGIGEPDCVVN
                     VGVSGPGVVANAVEAEPNADFGALASVIKRTAFKITRMGELVGRAASEKLRVPFGIVD
                     LSLAPTPAPGDSVARVLQAMGLERVGTHGSTAALALLNDAVKKGGAMASSYVGGLSGA
                     FIPVSEDAVMIDAAAAGTLTFDKLEAMTCVCSVGLDMIAVPGDTPATTLAAIIADEAA
                     IGMINRKTTAVRLIPAPGKKVGDVVSFGGLLGRAPVIALHRESSEAFIARGGRIPAPL
                     QALNN"
     gene            2858..>3833
                     /locus_tag="VM692_09770"
     CDS             2858..>3833
                     /locus_tag="VM692_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ52498.1"
                     /translation="MCSLLCLAALFVLGVEVASAQRSGASAAASTAAAEVEVVQLRDN
                     FYVIGGAGGNVLMQIGSEGVILVDSGTAARAADVLAAIRRITPLPIRYIINTSMDADH
                     VGGNEVLSKAGLSILPGAVAAGAGLGDDVLSNFGAASIFAHENVLTRMSSADPPIPSA
                     LWPTKTFFYRLYSMYLNGEGIQIIHTPAAHTDGNVIVFFRRGDVIATGDVIDTTRFPV
                     IDAARGGTLQGELDALNRLMDLSIHNVPLLWYPDRTFLVPGHGHVYDKLDLLEYRDAI
                     TIVRDRVQDLIDQGMTLAQVQAADPTLGYRSQYGRDSGPWTTEMFVAAV"
BASE COUNT          610 a         1275 c         1279 g          669 t
ORIGIN      
        1 aggcctcgtc gcgcgagcac gaggcgcttg cgcgcctgcg caaagcgacg aggtccgtgc
       61 cgaacagcgt gatgcagatc ggccccgatc agggccagct gatggcgctg ctcgttcggc
      121 tcatcggcgc ccggcgctgc atcgaggtcg gcacgtacac gggctattcg gcgctggccg
      181 ttgcgctcgc gctgccgccg caaggtcagc tcattacgtg cgacgtcagc gccgaatgga
      241 cgaacgtcgg tcggccgttt tggcgcgaag ccggcgtcga ggcgcgcatc gatttgcggt
      301 tgaagcccgc gctcgagacg ctcgacgagc tgctcgtgaa gggcgaggcc ggccgtttcg
      361 atttcgcgtt catcgacgcc gacaagtcga actacgtcgc gtactacgaa cgactgctgc
      421 agctcgtccg acagaacggg ctcatcgccg tcgacaacac gctcgcgctc gcgggcgcgc
      481 cgatcttcgc acaggagtcg gagagcgcga aggcgatgcg cgtcttcaac gagctcgtgc
      541 acgacgacga gcgtgtcgac atcgcgatgc tcacggtcgg cgaagggctg acactgctgc
      601 gcgtgcgctg acgcacaccg cacccgcgcg cgtgcaccgg caccgcgcgt gacgctctcg
      661 cgcgcgcctc atcgaggtgc gctgcgccgc aagtttcgtg cggcatttca cgatagaacc
      721 gcgcagttcc tagtgccgag cccgttggca cgcttgctgc ttagcgtcga ttgcgtatgc
      781 ggctctactc agcaaatcga ttttcgctag agcgcagcga cactcgcttg tcacattcgt
      841 tcggtacggt agcgctcatt caagacgttc gttgtgatgg ctcattgcaa ccaacgtaca
      901 tgtagggaaa cgacgaaatc ggttttcggt tcgcaaactg ccaaggaggc attccatgac
      961 aacttcagct cgttccgctc tgctactttt cgcggctctg ttgctcggtg gtctaccgcg
     1021 tcgatcggcg cgccgcgcgt cggtgaaatt gcgaccacga ccgtcaaggt cgcggatctc
     1081 gacatctcca ccgcgagcgg agccgaaacg ctgtacaagc gcatcgtgac ggctgcgcgt
     1141 gtcgtgtgct tcgacacgga tgtgaaagcc atgcacgcct gccgtgcgcg tgccgtcgcc
     1201 gatgccgtgg cgagggtcgg cagtccgctg ctgagcgccg cgcatcggtc gacgatcgag
     1261 cgcgcagagg aggtggtacg ccgctgaagc tcgcgttgcg cgcgcagccg gcaactcccc
     1321 cctgtcggtt gcgcgcgccg ctttttctcg ctggcggtca attgttcagg gcttgcagcg
     1381 gcgccggaat gcgcccgccg cgcgcaatga acgcttcgct cgattcgcga tgcaatgcga
     1441 tgacgggcgc gcgccccagc aagcccccga acgacaccac gtcgccgacc ttcttgcccg
     1501 gcgccggaat caaacgcacc gccgtcgtct tgcgattgat catgccgatc gcggcttcgt
     1561 ccgcgataat cgccgccagc gtcgttgccg gcgtatcgcc gggcacggcg atcatgtcga
     1621 gcccgacgga gcagacgcag gtcatggcct cgagcttgtc gaacgtcagc gtgccggctg
     1681 cggcggcgtc gatcatgacg gcgtcctcgg acaccgggat gaacgcgccc gagaggccgc
     1741 cgacgtagct cgacgccatc gcgccgcctt tcttgacggc gtcgttcaac agcgcgagcg
     1801 cggccgtcga gccgtgcgtg ccgacgcgct cgagccccat ggcctgcagc acgcgagcga
     1861 cgctgtcgcc gggcgccggc gtcggcgcga gcgagaggtc gacgatgccg aacggcacgc
     1921 ggagcttttc cgatgccgcg cggccgacga gctcgcccat gcgcgtgatt ttgaacgcgg
     1981 tgcgcttgat gacgctcgcg agtgcgccga agtcggcgtt cggctccgcc tcgacggcgt
     2041 tggcgacgac gccggggccg ctgacgccga cattgacgac gcagtcgggc tcgccgatgc
     2101 cgtggaatgc gcctgccatg aacgggttgt cttcgacggc gttgcagaac acgacgagct
     2161 tggcgcagcc gatgccgtcg cgatccgcgg tgcgtttcgc ggtggcgacg atgatctcgc
     2221 ccatgcgcgc gacggcgtcc atgttgatgc cggcgcgcgt cgtcgcgacg ttgatcgacg
     2281 cgcagacgcg ctcggtgcgc gcgagcgcct cgggtatcga ggcgagcaac gcggcgtcac
     2341 cgggcgtcgt gcccttgtgc acgagcgccg agaagccgcc caaaaagttg acgccgactt
     2401 cggcggccgc gcgctcgagg tactcggcga acagcacgta gtcgtcgctc gcggaggctt
     2461 cggcgacgag cgagaccggc gtcaccgaga cgcgcttgtt gatgattgga attccgtact
     2521 cacgctcgat ctcctcgccg acggcgacga gccgcgccgc cgcgcggcag atcttctcgt
     2581 aggctttctt ggcggcggcc tcgaggctcg cgtctgcgca attgcgcagg ctgatgccca
     2641 tcgtgatcgt gcggatgtcg agattgtcca tctgcaccat gcggatggtc tcgaggattt
     2701 cctcgggcga gaactggatg gccatgggtt gctacttggt gggacggagc ggattgtgcc
     2761 acgcggccgt gtgctgcttg actgcggcga tgcgcggtaa ccagaatggc cgcagtcaac
     2821 cgggccatca atgaagcgac cgctcgcaca aagacccgtg tgctcactac tctgcctcgc
     2881 cgcgctgttc gtgctcggcg tggaggtcgc gtcggcgcag cgcagtgggg cgagcgccgc
     2941 cgcgtcgact gccgcggccg aggtcgaagt cgtgcagctg cgcgacaact tctacgtcat
     3001 cggcggcgcg ggcggcaacg ttctcatgca gatcggttcc gagggcgtca tcctcgtcga
     3061 ctcgggcacc gcggcgaggg ccgccgacgt gctggccgca atccggcgaa tcacgccgct
     3121 gcccatccgc tacatcatca acaccagcat ggacgccgac catgtcggcg gcaacgaggt
     3181 gctctcgaag gcgggcttga gcatcctgcc gggcgccgtc gcggccggcg ccggcttggg
     3241 cgacgacgtg ctctcgaact tcggagcggc gagcatcttc gcgcacgaga acgtgctgac
     3301 gcggatgtcg tccgcagacc cgccgatccc gtcggcgctt tggccgacga agaccttctt
     3361 ctaccgcttg tactcgatgt acttgaacgg cgagggcatt cagatcatcc acacgccggc
     3421 cgcgcacacc gacggcaacg tcatcgtgtt cttccgccgc ggcgacgtga tcgcgaccgg
     3481 cgacgtcatc gacacgacac gcttcccggt catcgacgcg gcgcgcgggg gcacgctgca
     3541 aggcgagctc gacgcattga accggttgat ggacctgagc atccataatg tgccgctgct
     3601 gtggtacccc gaccgcacgt tcctcgtgcc gggccacggc catgtctacg acaagctcga
     3661 cctgctcgag taccgcgacg cgatcacgat cgtccgcgac cgcgtgcagg atctcatcga
     3721 tcaaggtatg acgctcgcgc aagtgcaagc ggccgatccc acgctgggtt atcgcagcca
     3781 gtacggccgc gattccggcc cctggacgac cgagatgttc gtcgcggccg tct
//
LOCUS       DATLEE010000453         3629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5728093, whole genome shotgun sequence.
ACCESSION   DATLEE010000453 DATLEE010000000
VERSION     DATLEE010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5728093"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VM692_09775"
     CDS             complement(<1..632)
                     /locus_tag="VM692_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HUQ52499.1"
                     /translation="MQSLFEKIWASHVVVPETDDTPAVLYVDLHLLHEVTTPQAFDML
                     RSKGLPVRRTDRCLATLDHSTPTVPVTTLKDLEVVSEPSAAKQVRQMEKNCEDFGIEL
                     RGFSSPDRGIVHVMGPELGATQPGATIVCGDSHTSTHGAFGALAFGIGTTEVGHVLAT
                     QCLLQRKPKTMAINVDGTLPPDVTAKDLILMIIGTIGVGGGTGHVLEYRG"
     gene            complement(642..1601)
                     /locus_tag="VM692_09780"
     CDS             complement(642..1601)
                     /locus_tag="VM692_09780"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HUQ52500.1"
                     /translation="MPLKTSKYIWHEGKLVPWEQATVHVLSHALHYGSSVFEGIRVYK
                     TPTGAAGFRLTDHVRRLYHSAKIYRIKIPFALDALVTACRDVVRENGLLNGAYIRPIA
                     YRGYGEMGVAGNIEQPASVSVAAWEWGSYLGEGGLDQGVDVCVSSWQRVAPNTVPALA
                     KAGGNYLSSALVTLEARRLGFAEGLALNTAGFVSEGAGETLLLIQDGKLYTPPVASSI
                     LSGITRNTVVRLAEANNLEVLEQNIPREMLYIADEVFLTGTAAEITPVRSVDKIPVGE
                     GKRGPITEKLQKQFFGLFDGTTADRWGWLEPIGDQKSRRPVAV"
     gene            complement(1644..2630)
                     /gene="ilvC"
                     /locus_tag="VM692_09785"
     CDS             complement(1644..2630)
                     /gene="ilvC"
                     /locus_tag="VM692_09785"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HUQ52501.1"
                     /translation="MKIYNEADVDAAALRGEKIAVLGYGSQGRAHAMNLRDSGYDVVI
                     GVRPDGNTMPTAQRDGFTVKAPAEAVKGVGLVAILAPDLAQAQLYKDIEPNLKKGAIL
                     LFAHGFNIIYKLVTPRADLDVILIAPKGPGRLVRRQYEEGRGVPCLMAVAQDVSGKAQ
                     ARALAYAHGIGGTRGGVLATTFKEETETDLFGEQAVLCGGATELIVRGFQTLVDAGYQ
                     PEVAYFECMHEMKLIVDLLHEGGLKKMHQYVSDTAKYGDLTRGPRIIDAHVRESMKQV
                     LKEVQDGTFAKQWIEENRTGQKNYKRLMQEDLDQGIEKVGQELRGRMSWLQE"
     gene            3089..3571
                     /locus_tag="VM692_09790"
     CDS             3089..3571
                     /locus_tag="VM692_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HUQ52502.1"
                     /translation="MTIKVGDKMPKGQFTRMGANGPEPVPTDDLFRGKKVVLFSVPGA
                     FTPTCSAKHLPGFVNNAAQLKAKGVDTIACMAVNDVFVMKAWGEHAKAAGKIEMLADG
                     NGEYARALGLELDASKFGMGQRSKRFSVIVDDGVVKTLNIEPPGEFGVSSAEAALKQL
                     "
BASE COUNT          636 a         1190 c         1145 g          658 t
ORIGIN      
        1 tcgccgcggt attcgagcac gtgccccgtg ccgccgccga caccgatcgt gccgatgatc
       61 atgaggatca ggtccttggc cgtgacgtcg ggcggcagcg tgccgtcgac gttgatggcc
      121 atggtcttcg gcttgcgctg cagcaagcac tgcgtcgcga gcacgtggcc gacctcggtc
      181 gtgccgatgc cgaaggcgag ggcgccgaac gcgccgtgcg tgctcgtgtg cgagtcgccg
      241 cagacgatcg tcgcacccgg ctgcgtcgcg ccgagctccg gccccatgac gtgcacgatg
      301 cctcgatcgg ggctcgagaa gccgcgcagc tcgatgccga aatcctcgca gttcttctcc
      361 atctgccgga cttgcttcgc ggcggacggc tcgctgacga cctcgaggtc cttcagcgtc
      421 gtgacgggca ccgtcggcgt cgaatggtcg agcgtcgcga gacagcggtc cgtgcgtcgt
      481 acgggcaggc ccttcgagcg cagcatatcg aacgcctgcg gcgtcgtgac ctcgtgcagc
      541 aggtgcagat cgacgtagag aacggccggc gtgtcgtccg tctcgggcac gacgacgtgc
      601 gaggcccaaa ttttctcgaa cagcgattgc acggccgcct cttagaccgc taccggccga
      661 cgggacttct gatcgccgat cggctcgagc cagccccagc ggtcggccgt cgtgccgtcg
      721 aacaagccga agaattgctt ctgcagcttt tcggtgatcg ggccgcgttt gccttcgccg
      781 acgggaatct tgtcgacgga gcgcacgggc gtgatctcgg ccgcggtgcc cgtcaggaac
      841 acctcgtccg cgatgtacag catctcgcgc gggatgttct gctcgaggac ctcgaggttg
      901 ttggcctccg cgagccgcac gacggtgtta cgcgtgatgc cggacaagat cgacgaggcg
      961 acgggcggcg tgtagagctt gccgtcttga atcaacaaca aggtctcgcc ggcgccttcg
     1021 ctgacgaagc ccgcggtgtt caacgctaac ccttccgcaa agcccaagcg gcgcgcctcg
     1081 agcgtcacga gggcgctcga cagatagttg ccgccggcct tggcgagggc agggaccgtg
     1141 ttcggcgcga cgcgctgcca tgacgacacg cagacgtcga cgccttggtc gagcccgcct
     1201 tcgccgaggt acgagcccca ttcccacgcg gcgacggaga cgctcgcggg ctgctcgatg
     1261 ttgccggcca cgcccatctc gccgtagccg cgatacgcga tcggccggat gtaggcgccg
     1321 ttcagcaaac cgttctcgcg cacgacgtcg cgacacgccg tcacgagcgc gtcgagcgcg
     1381 aacgggatct tgatgcgata gatcttcgcc gagtgataca gccggcggac gtgatccgtc
     1441 agacggaagc ccgcggcgcc ggtcggcgtc ttgtagacgc ggatgccttc gaataccgac
     1501 gagccgtagt gcagcgcatg gctcagcacg tgcaccgtgg cctgttccca cggcaccaat
     1561 tttccttcat gccaaatgta cttgctcgtc ttgaggggca tatccgctcc taggctacgg
     1621 tcttggacag tcgaatcgaa cgcctattcc tgcagccagc tcatgcggcc gcgcagctcc
     1681 tggcctactt tctcgatgcc ctgatcgaga tcctcttgca tcaagcgctt gtagtttttc
     1741 tgacccgtgc ggttttcctc gatccactgc ttcgcgaacg tgccgtcctg aacctccttc
     1801 aggacctgct tcatgctttc gcgcacgtgc gcgtcgatga tgcgcggccc gcgcgtcaga
     1861 tcgccgtact tggccgtgtc gctgacgtac tggtgcattt tcttgaggcc gccttcgtgc
     1921 agcaggtcga cgatgagctt catctcgtgc atgcattcga agtaggcgac ctcgggctgg
     1981 tagcccgcgt cgacgagcgt ctggaagccg cgcacgatca gctcggtcgc gccgccgcag
     2041 agcacggcct gctcgccgaa caaatccgtc tcggtctctt ctttgaacgt cgtcgcaagc
     2101 acgccgcccc gggtgccgcc gatgccatgc gcgtaggcga gcgcgcgggc ttgcgctttg
     2161 ccgctcacat cttgcgcgac ggccatgagg catggcacgc cgcgcccttc ctcgtactgg
     2221 cgacgcacga gacggcccgg gcccttcggc gcgatcaaga tcacgtcgag atcggcgcgc
     2281 ggcgtcacga gcttgtaaat aatgttgaag ccgtgagcga aaaggaggat cgcacccttc
     2341 ttcaagttcg gctcgatgtc cttgtagagc tgcgcttgcg cgaggtccgg cgcgaggatc
     2401 gcgaccaagc cgacgccttt gacggcttcc gccggcgcct tgacggtgaa cccgtcgcgc
     2461 tgcgccgtcg gcatcgtgtt gccgtcgggt cggacgccga tgacgacgtc gtagccggaa
     2521 tcgcggaggt tcatcgcatg ggctcggccc tggctgccgt aaccgagcac tgcaatcttc
     2581 tcgccgcgca gcgccgctgc atcgacatcc gcttcgttat aaatcttcat cgcactctcc
     2641 aaaaaacaac ttcgcccatt caaaactaaa agaggtactc gttgggaagc aaaatcttgc
     2701 accggaaaaa aaaaccccgc aggcctttcg ggtgcggggt ttatctaatg aagcttcgtc
     2761 gtcaagccaa acgcccgcac ctttcgcggt tgcgtactac cgagagcgcg gagcgggtgg
     2821 ttttcatctc agtccgatga agaatcgagc gcgcatgatc ccggaaaaga ccaaacactg
     2881 tcaacgcctt aggcgacata gcaggctcgc cggccctacg gctccgcggc gccggccgcc
     2941 gcttggaccg gaatggccca cccgccgccc tcgagcgcgg ccccgaacgg cacgctatga
     3001 ccaaaaggcg gccgacctat aataccggcc ccgcccgcgg cccccttcgg cagcgggcat
     3061 ccatagtgct tttaaggagc gaacaacaat gaccatcaaa gtcggcgaca agatgccgaa
     3121 aggccagttc acacggatgg gggcgaacgg cccggagccg gtgcccacgg acgatctctt
     3181 ccgcggcaag aaggtcgtgt tgttctccgt ccccggcgcc ttcacgccga cgtgctcggc
     3241 caagcatctg ccgggtttcg tcaacaacgc cgcgcagctg aaagccaagg gcgtcgacac
     3301 gatcgcctgc atggccgtca acgacgtgtt cgtcatgaag gcttggggcg agcacgccaa
     3361 agccgccggc aagatcgaga tgctggccga cggtaacggt gaatacgcgc gtgccttagg
     3421 cctcgagctc gatgcgagca agttcggcat gggccagcgc agcaagcgct tctcggtcat
     3481 cgtcgacgac ggcgtcgtga agacgctgaa catcgagccg cccggcgagt tcggagtgag
     3541 ctcggcggaa gccgcgctga agcaacttta agattggcgc gagccgcgcg cacggagtgc
     3601 gccgatcagc tcgggtatgg cttggaaca
//
LOCUS       DATLEE010000454         6295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4216686, whole genome shotgun sequence.
ACCESSION   DATLEE010000454 DATLEE010000000
VERSION     DATLEE010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4216686"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VM692_09795"
     CDS             complement(<1..566)
                     /locus_tag="VM692_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HUQ52503.1"
                     /translation="MSAKKAAAKKKATASKKKLAKKAPVRSSKAKLKSAHKPSAKTGK
                     SAKPTSNKKTPLKLAVPKAQPKPSGKKTIATAPKKPGSGPLTPVRVASKPAPLPPPKR
                     RSSGSVLSGPVKGFTPYQPKRGEEYMNKDQLEHFRQLLLAWKRELMEEVDRTMLHMKD
                     DAANFPDPNDRATQESDFGLELRTRDRER"
     gene            complement(642..1058)
                     /gene="queF"
                     /locus_tag="VM692_09800"
     CDS             complement(642..1058)
                     /gene="queF"
                     /locus_tag="VM692_09800"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HUQ52504.1"
                     /translation="MATKPAARPSKKSLETFPNPSSDRDYTIRMRIPEFTCLCPKTGQ
                     PDFATLHLEYVPDVTCVELKSLKLYVWSYRNEGAFHEAVTNRILDDLVDLMKPRFIRL
                     TAEFNVRGGIYTEVVAEHRQRGWKAAPAVELPDSAR"
     gene            1191..4694
                     /gene="smc"
                     /locus_tag="VM692_09805"
     CDS             1191..4694
                     /gene="smc"
                     /locus_tag="VM692_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HUQ52505.1"
                     /translation="MRLSKIKLAGFKTFVDSTTIKFPSNLMGIVGPNGCGKSNIIDGI
                     RWVLGEGSAKTLRGDSMVDVIFNGSSTRKPVGIASIELTFDNSDGTITGAFAGYNEVS
                     VRRVVSRDGTSQYYLNNTRCRRKDITHILLGTGVGSHGYSIIEQGMISRLVEAKPEEL
                     RAFLEEAAGISKYKERRRETEHRIQHTRENLERLKDLREEIEKQLTHLQKQAKDAERY
                     KNLKAEQRKVNAELLALRLGALRKEVADQEHALREKQIALEAAVAAQRSAETSIEKLR
                     VELADRNERFSVVQGGYYKVGAEIARLEQSLQHRKDLMQRQTEDLQSTDQQVAEINAH
                     VASDQIELEELERVLGELGPGLEQAYAIQRSSQQTLEHAERAMEQARAEWERLSEDLA
                     SAARSIEVEDTRLEQLTAQGQRIEKEQHRRQEERHALSSAEVEQRLETLVAQEERLAA
                     ACENASRALETVWTQIQQLREQEKRISEHLDQLRESLQTERGRLTSLEALQEGALGKS
                     TEQVNRWLQAQTLDERRRLAQDLIVESGWERAVETVLGPYLQAVGINSIDTVAGSLAD
                     LTEGGLTLIETDGAAAERPTGDALLARMQAPAAVSSLLVGVRVADSVQEAIARRNELA
                     DGESFVTREGVWVGRRWLRMNRSDDPQVGVIARGDEIKRLRESTQTVARRADEVAKAL
                     ADTRFQLERLEEARAQAQAEAARRQELFADTKAKLGASRAELDQARQRAAALDRAIAD
                     LATEQQALITAVDESKARRAAAVSRQNGLAASREGVEQQRREHQERVAAARASAEQHR
                     EEAQAIAIKVESRRSSRESASAALLRVQSQLAHLTKRRQELNAELLAAAEPLRADEQT
                     LVAKLDERLLVEADLGTARQAMDDIDAQLRETEQQRNERQQAVNEIRELADNVRLAVR
                     ESQVRAETVGEQFAATGFILDEITLGLPADATAAVWNETFESIERKIQRLGAINLAAI
                     DEFQEQSERKKYLDAQFTDLTEALQTLENAIRKIDRETRTRFQETFDKANQGLGRLFP
                     RLFGGGHAYLELQGEDVLEAGVTIMARPPGKKNSTIHLLSGGEKALTAVALVFSIFEL
                     NPAPFCLLDEVDAPLDDANVGRFSEIVKEMSEHVQFVLITHNKTTMEAMHQLTGVTMQ
                     EPGVSRMVAVDIDEAVRLAAM"
     gene            4733..5356
                     /locus_tag="VM692_09810"
     CDS             4733..5356
                     /locus_tag="VM692_09810"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HUQ52506.1"
                     /translation="MGLGALVLVGVYLWSRGIVSRDMLPALPRRKQRTEPSIGEPAAP
                     PPPPPPPPETPSLVETVSEEEKARPPPDRIVALRLVPRAGELPTERAVAALRSAGLEH
                     GRYAIFHRQLGTNREGFSVASLTEPGSFDVEHLGNATIAGLSFFVVLPGGGDPVARFD
                     AMVETARRLSVELAADLFDERGSSWSSQRERYLREELIEYRHQFERR"
     gene            5367..>6295
                     /gene="ligA"
                     /locus_tag="VM692_09815"
     CDS             5367..>6295
                     /gene="ligA"
                     /locus_tag="VM692_09815"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HUQ52507.1"
                     /translation="MERSPSSLPKLRSELAELRRTIEAHNHSYHVLDNPTVPDAEYDR
                     LMRRLQEIERQHPELVTPDSPTQRVGATKLEAFREIRHAKPMLSLDNVFNDEELGAFD
                     QRVRDRLGDADIDVERVIYWAEPKLDGAAISLRYENGELVFAATRGDGATGEDVTHNV
                     RTIRSIPLRLRGSKPPAVFEVRGEVFMPRAGFLEFNKRAVERGEKTFVNPRNAAAGTL
                     RQLDPRLAASRPLDAFFYALGEVSDGSLPAKREQSAIVGMLRDFGLRVCPEAELADGV
                     HGCLDYYRRIGRQRSALPYDIDGVVYKVNKLEW"
BASE COUNT         1186 a         1945 c         2092 g         1072 t
ORIGIN      
        1 cgctctctat ctcgagtgcg gagctcgagc ccgaagtccg actcctgggt cgcgcggtcg
       61 ttgggatcgg ggaagttcgc tgcatcgtcc ttcatgtgca gcatcgtgcg atcgacttcc
      121 tccatgagct cgcgcttcca ggcaagcaac agctgccgga aatgctcgag ctgatccttg
      181 ttcatatatt cctcgccgcg tttcggctga taaggcgtaa agcccttcac cggaccgctg
      241 aggacactgc cgctgctgcg ccgtttcggc ggcggcagcg gggcgggttt agacgccacg
      301 cgaacgggcg tcagcgggcc ggagcccggt tttttgggtg cggtagcgat cgtcttcttc
      361 cctgatggtt tgggttgcgc cttcggtacg gccagcttga gcggcgtctt cttgttgctc
      421 gttggcttgg ctgacttccc tgttttggcg gacggcttat gagcggactt gagcttggcc
      481 ttgctggagc ggacgggagc tttcttggcc agcttcttct tgctggcggt cgcctttttc
      541 ttggcagctg ctttttttgc agacatggcc ttggcctcca agaccgttca aaggagcggg
      601 cgattataca gaggcgccga agtagcggaa gggggcgcag ctcagcgtgc ggagtccggc
      661 aactcgaccg ccggcgccgc cttccaacct cgctgccggt gttcggcaac aacctccgtg
      721 taaatgccgc cgcggacgtt gaattcggct gtcaggcgaa tgaatcgcgg cttcatcagg
      781 tccacgagat cgtcgaggat gcgattggtc accgcttcgt ggaacgcgcc ctcattgcga
      841 tacgaccaca cgtagagctt cagcgatttc aactcgacgc aggtgacatc gggcacgtat
      901 tcgagatgca gcgtcgcgaa atccggctga cctgtcttcg gacagagaca ggtgaactcc
      961 ggtatccgca tccgtatcgt gtaatcgcga tcggagctcg ggttgggaaa cgtctcgaga
     1021 gattttttcg acggccgagc cgcgggctta gtcgccacga ttttgtgttc cgattgagcg
     1081 tgtgcagcga cgcgcgcggc cgtcgctttt ggattggttt ttactctaca ctaccgccgt
     1141 cgaggcggcc atgcgccctc tcttacactc gaccatcacg gggtcatcca ttgcgcctga
     1201 gtaagatcaa gctcgcgggc ttcaagactt tcgtcgattc gaccacgatc aaattcccga
     1261 gcaacctgat ggggattgtc gggccgaacg gctgcggcaa atccaacatc atcgacggca
     1321 tccgctgggt gctcggcgaa gggtctgcaa agacgctgcg cggcgactcg atggtcgacg
     1381 tcatcttcaa cggctcgtcg acgcgcaagc ccgtcggcat cgcgagcatc gagctcacgt
     1441 tcgataactc cgatggcacg atcacgggtg cgttcgcggg ctacaacgaa gtgtccgtgc
     1501 gccgcgtcgt ctcgcgcgac ggcacctcgc agtactactt gaacaacacg cgctgccgcc
     1561 gcaaagacat cacgcacata ctgctcggca ccggcgtcgg ctcgcacggc tactcgatca
     1621 tcgagcaggg catgatctcg aggctcgtcg aggcgaagcc cgaggagctg cgggcgttcc
     1681 tcgaggaagc ggctggtatc tcgaagtaca aagagcggcg ccgcgagacc gagcatcgca
     1741 ttcagcacac gcgtgagaac ctcgagcgcc tcaaggacct ccgcgaggag atcgagaaac
     1801 agctcacgca cctgcagaaa caggcgaagg acgcagagcg ctacaagaac ctcaaggccg
     1861 agcaacgcaa ggtcaacgcg gaattgctcg ctttgcgctt gggtgcgcta cgcaaggagg
     1921 tcgccgacca ggagcatgcg ctgcgcgaga agcagatcgc gctcgaagcg gccgtcgcgg
     1981 cccagcgctc ggccgaaacg tcgatcgaga agctgcgcgt cgagctggcc gatcgcaacg
     2041 agcgctttag cgtcgtgcag ggcggctatt acaaggtggg cgccgagatc gctcgcctcg
     2101 agcagtcgct gcagcatcgt aaggacctca tgcagcggca gaccgaggac ctgcagagca
     2161 cggaccagca ggttgccgag atcaacgcgc acgtcgcgag cgatcagatc gagctcgaag
     2221 agctcgagcg cgtgctcggc gagctcgggc cggggctcga gcaggcctac gcgatccagc
     2281 gcagctcgca gcaaacgctc gagcatgcgg agcgggcaat ggagcaggcc cgcgcggagt
     2341 gggagcgcct cagtgaggac ctggcctctg ccgcccggtc gatcgaggtc gaggacaccc
     2401 gcctcgagca attgacggcg cagggtcagc gcatcgagaa ggaacagcac aggcgccagg
     2461 aggaacgtca tgcgttgagc tccgccgagg tcgagcagcg gctggaaacg ctggtcgcgc
     2521 aggaagagcg cctggccgcg gcatgcgaga acgcaagtcg cgcgctggaa accgtgtgga
     2581 cgcaaattca acagctccgc gagcaagaga agcgcatctc ggagcacctc gatcagctgc
     2641 gcgagagcct acagaccgag cgcgggaggc tgacgtctct cgaggcgctg caagagggcg
     2701 ccctcggcaa gtcgacggag caagtcaacc ggtggctcca agcacagacg ctcgacgagc
     2761 gccgccgctt ggctcaggac ctcatcgtcg agtcgggttg ggaacgggcc gtcgagacgg
     2821 tgctcgggcc gtatctgcaa gccgtcggta tcaacagcat cgacacggtc gcgggctcgc
     2881 ttgcggatct cacggaaggc ggtctaacgc tgatcgagac cgacggagcg gctgccgagc
     2941 gaccgacggg tgacgctttg ctcgcgcgca tgcaagcgcc ggcggcggtc tcgtcgctgc
     3001 tcgtcggagt tcgagtcgcc gattccgtcc aagaggcaat tgcacgccgc aacgagctcg
     3061 ccgatggcga gtcgttcgtg acgcgtgagg gcgtttgggt tggacgtcga tggctgcgca
     3121 tgaaccgcag cgacgatccg caagtcggtg tcatcgcacg cggtgacgag atcaaacgcc
     3181 tgcgcgagag cacgcaaacg gtggctcggc gcgccgatga agtggccaag gcgctggccg
     3241 acacgcgttt tcagctcgag cggctcgaag aagcgcgtgc gcaggcccag gccgaagcgg
     3301 ctcgccgaca agagcttttt gccgatacga aggcgaagct tggcgccagt cgcgcggagc
     3361 tcgaccaggc gcgccaacga gccgcggcgc tcgatcgggc gatcgccgat ctcgcgaccg
     3421 agcagcaagc tctgattacg gccgtcgacg agagcaaagc gcgacgcgcc gcagcggtca
     3481 gccgtcagaa cggcttggcc gcatcgcgtg aaggtgtcga gcagcagcgg cgcgagcacc
     3541 aagaacgcgt ggccgcggcg cgcgcgagcg ccgagcagca tcgcgaggaa gcgcaggcga
     3601 tcgccatcaa ggtcgagtcg cggcgcagct cgagggaatc cgcgagcgcg gcgctgcttc
     3661 gggtgcagag ccagctcgcg catctgacga agcggcggca agagctcaat gcggagctgc
     3721 tggcggccgc cgagccgctg cgtgcggacg agcaaacgtt ggtcgccaag ctcgacgaac
     3781 gcctgctcgt cgaggccgat ctcggtacgg cgcggcaagc gatggacgac atcgacgctc
     3841 agttgcgcga gaccgagcag caacgcaacg agcgtcaaca ggccgtcaac gagattcgcg
     3901 agcttgccga caacgtccgg ctcgcggtgc gcgaatcgca ggttcgagcg gagaccgtcg
     3961 gcgagcagtt tgccgcaacg ggtttcatac tcgacgagat cacgctcggt ctgccggccg
     4021 atgcgacagc cgccgtttgg aacgagacct tcgagtcgat cgagcgcaag attcagcgcc
     4081 tcggcgccat caatctggcg gccatcgacg agtttcagga gcaatcggag cgcaaaaaat
     4141 atctcgacgc gcagttcacg gacctcaccg aagcactgca gacgctcgag aacgccattc
     4201 gcaagatcga tcgcgagacg cgcacgcgct tccaagagac gttcgacaag gcgaatcaag
     4261 ggctcggccg gctgtttccg cgcctcttcg gcggtggcca cgcttacctc gagttgcaag
     4321 gtgaggatgt gctcgaggcc ggcgttacga tcatggcgcg cccgccgggc aagaagaaca
     4381 gcacgatcca cttgctgtcg ggcggcgaga aggcgctgac cgccgtggcg ctcgtgttct
     4441 cgatcttcga gctcaatccg gcgccgttct gcctcctcga cgaggtcgac gcaccgctcg
     4501 acgacgcgaa cgtgggccgc ttctcagaga tcgtcaaaga aatgtcggag cacgtgcaat
     4561 tcgtcctgat cacgcacaac aagaccacga tggaggcgat gcatcagctc accggcgtga
     4621 cgatgcagga gcctggcgtg tcgcgcatgg tggcggtcga tatcgacgaa gcggtcagac
     4681 tcgcagcgat gtagccgtat agggcgctat gtgggacttg cgctgggtac tcttggggct
     4741 aggtgccctc gtacttgtcg gagtgtatct ctggagccgc ggcatcgtgt cgcgcgacat
     4801 gctacccgcg ctgccgcgcc gcaagcagcg tactgagccc agcattggag agcccgcggc
     4861 gccgcccccg ccgccgccgc cgccgccgga gacaccttcg ctcgtcgaaa cggtctccga
     4921 ggaagaaaag gcacggccgc ctcctgatcg catcgtcgcg cttcgactgg ttcctcgtgc
     4981 cggcgagctc ccaaccgagc gcgccgtggc cgcgctgcgc agcgcggggc tcgagcacgg
     5041 tcgttacgcg atctttcatc gccagctcgg aacgaatcgt gaggggttca gcgtcgcgag
     5101 cctaaccgag cccggctcgt tcgatgtcga gcatctaggc aatgcgacga tcgccggtct
     5161 cagcttcttc gtcgtgctgc cgggcggcgg cgacccggtt gcgcggttcg atgcaatggt
     5221 ggagacggcg cggaggctat ccgtagagct ggctgcagat cttttcgacg agcgcggcag
     5281 ttcgtggagc agtcagcgtg agcgctacct cagagaagag ctaatcgagt atcgccatca
     5341 gttcgagcga cgctgaggcg tggagcatgg agcgctcgcc ttccagcctc ccgaagttgc
     5401 gatccgagtt ggccgagctg cgccgaacga tcgaagcgca caatcattct taccacgtgc
     5461 tcgacaatcc gacggtgccg gatgccgagt acgaccgcct gatgcggcgg cttcaggaaa
     5521 tcgagcgcca gcatcccgag ctcgtcacgc cggattcccc aacacagcgc gtcggcgcca
     5581 ccaagctcga ggcgtttcgc gagattcggc acgcgaaacc gatgctttcg ctcgacaacg
     5641 tgttcaacga cgaggagctc ggcgcgttcg accaacgtgt gcgcgatcgt ttaggcgacg
     5701 cggacatcga cgtcgagcgt gtgatttatt gggccgagcc caagctcgac ggtgccgcga
     5761 tcagcttgcg ctacgagaat ggcgagctcg tgttcgctgc aacgaggggt gacggcgcga
     5821 ccggcgagga tgtcacgcac aacgtgcgca cgatccgctc gattccgctg cgcttgcgtg
     5881 gcagcaagcc accggcggtc ttcgaggtac gcggcgaggt cttcatgccg cgagcgggct
     5941 tcctggagtt caacaaacgt gcagtggaac gcggcgagaa aactttcgtg aatccgcgca
     6001 acgcggcagc cggcaccctg cggcaactcg acccacgact tgccgcttcc cggcccctcg
     6061 atgccttttt ctacgcgcta ggggaggtca gcgacggctc gctgccggcc aagcgtgagc
     6121 aaagcgccat cgtcggcatg ctccgcgact ttggattgcg ggtttgtccg gaagcggagc
     6181 ttgctgacgg cgtccacggc tgcctcgatt actaccggcg cattgggcgg cagcgctccg
     6241 cgctgccgta cgacatcgac ggggtcgtat ataaggtgaa caagctcgag tggca
//
LOCUS       DATLEE010000455         3567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4982686, whole genome shotgun sequence.
ACCESSION   DATLEE010000455 DATLEE010000000
VERSION     DATLEE010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4982686"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /gene="otsB"
                     /locus_tag="VM692_09820"
     CDS             <1..668
                     /gene="otsB"
                     /locus_tag="VM692_09820"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HUQ52508.1"
                     /translation="PDAVRVTEPLRELLGALLEYNGGAVALVSGRTLADLDQLFAPLA
                     FPAAGLHGIERRSAQGMIHTHVDSAALRAVRDFLSEDSCEGLLVEDKGGAIAIHYRER
                     PDLAMQAQELARRAAQIAGPRWEAVPGKMSIEIRDGNVGKHHALERFMSEAPFSGRIP
                     AFLGDDHTDEDGFEFVNRHGGWSVHIGSGPTCARSNLPDPMAVWRLLRALSPRHAQEH
                     LRA"
     gene            complement(713..2980)
                     /locus_tag="VM692_09825"
     CDS             complement(713..2980)
                     /locus_tag="VM692_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HUQ52509.1"
                     /translation="MSTYLPEFMQKALLQRKAEAAELSRRGFIKLTGLAGGGLVLAMS
                     LSTTARKALAQPSGAASFEANPYVQIQPNGKIVLFAKNPDVGQGVKTSLPMIVAEELD
                     AAWADVEVRQSVIDAALYGPQFAGGSLSIPMNYDTLRRAGATARAMLVAAAAKNWGVP
                     AAELTTENSRVRHAASNRSATYGELAVAASTLAVPDAKTIALKPKSSFRLMGQRVTGV
                     DNAKLVRGEPLFGIDQRVPGMLFATYAKAPAIGARAVSANLDHIKTLRGVKNAFIVAH
                     QGDPIGFNPAAAAVLSGVAIVADSTWNAMQAKKALEVQWDAKEASSDSWTAAVAQAKS
                     LATKPAAQVLGEGGNVESAFAAGKTVEALYTYHYVSHADLEPQNCTAWFKGDSIEIWA
                     PTQTPQNAVDAVAELLKLPKEKVTLHQLRGGGGFGRRLANDSVVEAAIISKQAGGIPV
                     KVQWSREDDMAFDYYRPGGFHAQKASIDAAGKLSAYQNHFITFTADGKAPVSAADLAK
                     NEFPANVLANQRMVQSMIPSKIPTGPWRAPGSNVIAFCVQSFLNECAVAAKRDYVDVL
                     VEVMGAPRETAGGPFGAGLNTGRAVNVIKAVAERSGWGKQLPAGRGLGMAFHFSHQGH
                     FAEIAEVSVDKNNKVTVHKMTVVGDIGPIVNLSGAENQVQGCIVDAISTLGLAVTFEN
                     GRIEQGNFDKYPMGRISVAPPDLDVHFLDTNYPPTGVGEPAFPPAAPAICNAIYAATG
                     KRIRTLPITAEGFSI"
     gene            complement(2977..3462)
                     /locus_tag="VM692_09830"
     CDS             complement(2977..3462)
                     /locus_tag="VM692_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HUQ52510.1"
                     /translation="MLSLTINGRAHTVGDEVDPQTPLLWVLRDTLGLVGTKYGCGVGQ
                     CSACTVHLNGSAIQSCQVPASAAAGQQITTIEGLAPNGAALTAVQQAWIEEDVAQCGY
                     CQAGQMMRATALLARTPAPTDDDIDRDMAANICRCGTYTRIRKAIHNAAGKIAKNGGA
                     R"
BASE COUNT          535 a         1223 c         1180 g          629 t
ORIGIN      
        1 gtcccgacgc cgtgcgtgtg accgaacccc tgcgcgaatt gctcggcgcc ctgctcgagt
       61 acaacggcgg tgccgtcgcg ttggtcagtg gccgaaccct ggccgacctc gaccagctgt
      121 ttgcgccgct cgcgttcccc gcggcgggcc tgcatggaat cgaaaggcgc tctgcccagg
      181 ggatgataca cacgcacgtg gactctgcgg cgctgcgcgc cgtacgcgat ttcctgtcag
      241 aagattcctg cgaagggctg ctcgtcgaag acaagggcgg cgccatcgcg atccactatc
      301 gggagcggcc ggacctcgcg atgcaggcgc aggagctcgc acgacgggcg gcgcagatcg
      361 caggtccgag atgggaagcg gtgcccggca agatgtcgat cgagatacga gacggcaacg
      421 tgggcaagca ccacgcgctc gaaaggttca tgagcgaggc gccgttttcc gggcggatcc
      481 ccgcgttcct cggtgacgat cacaccgacg aggacggctt cgagttcgtc aaccgccacg
      541 gcggctggtc ggtgcacatc ggctcgggcc ccacgtgtgc gcgcagcaac ctgcccgatc
      601 ccatggccgt gtggcgcctg ctgcgcgcgc tcagcccgcg tcacgcccag gaacacctgc
      661 gcgcttgagc gaggtctggg ggagctcccc cagacctgct ctcgtcggga tcctagatgc
      721 tgaaaccctc tgccgtgatc ggcagcgtgc ggatgcgctt gcccgtcgcc gcatagatcg
      781 cgttgcagat cgcaggcgcg gccggcggga acgccggttc gcccacgccc gtcggcgggt
      841 agttcgtatc gaggaagtgc acgtcgagat ccggcggcgc caccgagatg cgacccatgg
      901 ggtacttgtc gaagttgccc tgctcgatgc gaccgttctc gaaagtgacc gcgaggccga
      961 gcgtgctgat cgcatcgacg atgcagccct gcacctggtt ctcggcgccc gacaggttga
     1021 cgatcggacc gatgtcgccg accacggtca tcttgtgcac ggtgaccttg ttgttcttgt
     1081 cgacgctcac ttccgcgatc tcggcgaaat gcccctggtg gctgaagtgg aacgccatgc
     1141 cgagcccacg cccggccggc agctgcttgc cccagcccga acgctcggcg acggccttga
     1201 tgacgttgac cgcgcggccc gtgttgagcc cggcgccgaa cggaccgccc gccgtctcgc
     1261 gcggcgcgcc catgacctcg accaagacgt cgacgtagtc gcgcttcgcg gcgacggcgc
     1321 actcgttcag gaagctctgc acgcagaacg cgatgacgtt gctaccgggc gcgcgccacg
     1381 gccccgtcgg aatcttcgac ggaatcatcg actgcaccat gcgctgattg gccagcacgt
     1441 tcgcgggaaa ctcgttcttc gcgagatccg ccgcggacac cggcgccttg ccgtcggcgg
     1501 tgaacgtgat gaagtggttc tggtacgccg acagcttgcc ggcggcgtcg atcgaagcct
     1561 tctgcgcgtg gaagccgccg ggccggtagt agtcgaacgc catatcgtcc tcgcgcgacc
     1621 actgcacctt cacggggatg ccgccggctt gcttcgagat gatcgccgct tcgacgacgc
     1681 tgtcgttcgc gagccggcgg ccgaagccgc cgccgccgcg caactgatgc agcgtgacct
     1741 tctctttcgg caacttcagc aactcggcca cggcatcgac ggcgttctgc ggcgtctgcg
     1801 tcggcgccca gatctcgatg ctgtcgccct tgaaccaagc cgtgcagttt tgcggctcga
     1861 ggtccgcgtg cgagacgtag tgatacgtgt acagggcctc gacggtcttg cccgccgcga
     1921 acgcggactc gacgttgccg ccttcgccga gcacttgcgc ggccggcttc gtcgccagcg
     1981 acttcgcttg cgcgacggct gccgtccaac tatcgctcga cgcttctttc gcgtcccatt
     2041 gcacttcgag cgctttcttc gcctgcatgg cgttccatgt cgaatcggcg acgatcgcga
     2101 cgcccgacag cacggccgcc gcggccggat tgaagccgat aggatcgcct tgatgcgcga
     2161 cgatgaacgc gttcttgacg ccgcgcagcg tcttgatgtg atcgagattc gcgctgacgg
     2221 cacgcgcgcc gatggccggc gccttcgcgt aagtcgcgaa cagcatgccg ggcacgcgtt
     2281 ggtcgatgcc gaatagcggc tcgccgcgca cgagcttcgc gttgtcgacg cccgtgacgc
     2341 gctgacccat caagcggaag ctcgacttcg gcttcagagc aatcgttttt gcgtcgggca
     2401 ctgccagcgt cgaggcggcc acggcgagct cgccgtaggt cgcgctgcga ttgctcgcgg
     2461 catggcgcac gcggctgttc tcggtcgtca gctcggccgc cggcacgccc cagttcttgg
     2521 ccgcggcggc gacgagcatt gcacgcgccg tcgcgccggc gcggcgcaac gtgtcgtagt
     2581 tcatcgggat cgacaacgag ccgccggcga attgcgggcc gtagagcgcc gcgtcgatga
     2641 cggactgccg cacctcgacg tcggcccaag ccgcgtcgag ctcctcggcg acgatcatcg
     2701 gcagcgatgt cttgacgccc tgcccgacgt cggggttctt cgcgaacagc acgatcttgc
     2761 cgttcggctg gatctgcacg taggggttcg cctcgaacga cgccgcgccg ctcggttgcg
     2821 ccaacgcttt gcgcgccgtc gtcgacagcg acatcgcgag cacgagcccg ccgccggcga
     2881 gtcccgtcag cttgatgaag ccacgacggc tcagctcggc tgcttcggcc ttgcgttgca
     2941 gcagcgcttt ctgcatgaac tccggtagat aggtgctcat cgcgcacctc cgttcttcgc
     3001 gatcttgccc gcggcgttat ggatcgcctt gcggatgcgc gtgtacgtgc cgcagcggca
     3061 gatgttcgcc gccatgtcgc ggtcgatgtc gtcgtccgtc ggcgccggcg tgcgcgcgag
     3121 taacgccgtc gcgcgcatca tctggcccgc ttggcagtag ccgcactgcg cgacgtcttc
     3181 ctcgatccac gcttgctgca cggccgtcag cgccgcaccg ttcggggcga gcccctcgat
     3241 cgtcgtgatt tgctggccgg ccgcagccga cgcgggcact tggcaggatt gaatcgccga
     3301 gccgttcaaa tgcacggtgc acgcgctgca ctgaccgacg ccgcaaccgt acttcgtgcc
     3361 gacgagcccg agcgtgtcgc gcagcaccca tagcagcggc gtctgcgggt cgacttcgtc
     3421 gccgacagtg tgcgcgcggc cgttgatcgt gagactcagc attgggtgcc tccttaaggc
     3481 aagacgacga ggccgcggac gacgcgtccc ggcgcagccg caagtctacc aggtgccggt
     3541 gttacggctt cggtcgagcc gaaagcg
//
LOCUS       DATLEE010000456         4776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8455524, whole genome shotgun sequence.
ACCESSION   DATLEE010000456 DATLEE010000000
VERSION     DATLEE010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8455524"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VM692_09835"
     CDS             <1..368
                     /locus_tag="VM692_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52511.1"
                     /translation="EVGETWQASSAELALNGLDKGALEQTRTVIPGTAIDVVLSDFAL
                     TEAAVPAKPFRWRIQDVGSPQFQLEVQGLDVLLERTAAAGYRFLSIDARPIQRPFGRF
                     VFLRDADDILVEYAEPAAP"
     gene            438..611
                     /locus_tag="VM692_09840"
     CDS             438..611
                     /locus_tag="VM692_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HUQ52512.1"
                     /translation="MAGKIDQVKGKINEGIGRHKEKKGHENGDPLLEDEGTAQKVKGK
                     LQNVKGKVKDAID"
     gene            complement(677..2044)
                     /locus_tag="VM692_09845"
     CDS             complement(677..2044)
                     /locus_tag="VM692_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUQ52513.1"
                     /translation="MGDASKSPKGGAASKSPKAGDASKSPKGGPQHRLHAVAGVDTVV
                     VGAGHSGLAMSRMLAARGVEHVVLERGEVANAWRTERWDSLRLLTPNWQSRLPGYRYS
                     GPDPDGYMTSAEVGDFIAAYAQRSGAPVRTGTTVQSVRAAGDGYVVETDRATWRCRAV
                     VLAHGAHGVPNVPELAARLPRSVRTLTANEYRSPAMLDFGRVLVVGGSATGLQLADEI
                     RRSGRPVTLAVGEHIRMPRLYRGRDVQWWLDAAGVLDERHDEVDDLVRARRVPSPQLL
                     GSPERRTLDLNALREQGVELVGRLAGTDGSRLLFSGSLTNECAMADLKLARLLERFDD
                     WAAERRIGDAVGAPERFAPTRVATSPRGMLDLAKEPIATIIWATGFRPDYSWLHVPVL
                     DARGRLRHEGGVVAAPGLYTLGLNFMRRRKSSYMHGAEADARDLSAHLLAHLRGAHPR
                     EQCVA"
     gene            complement(2044..2604)
                     /locus_tag="VM692_09850"
     CDS             complement(2044..2604)
                     /locus_tag="VM692_09850"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUQ52514.1"
                     /translation="MNVTAIKVDNGVNVEALIGARAALTEAPAAARFKWRATCDWVRG
                     THSRTTVQEFFGLGAEQTRPKAFHYDADHPEVFAAEDNGATPVEYVLVALASCLTAGI
                     ASVAQNRNIQLRSVKATLEAGMDMQGILGIDSDVRNGFDGIKVTFRIDADASRAEIAA
                     LVAQSQRRSAVFDIITNPTNVAVEVA"
     gene            complement(2730..3251)
                     /locus_tag="VM692_09855"
     CDS             complement(2730..3251)
                     /locus_tag="VM692_09855"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HUQ52515.1"
                     /translation="MTVSLVLERTFDEPLTAEDVVDGGRESAQCFDLYRVTWHGSFLA
                     SDGRALICRFSAADMESARLALRDPDVDLSRFWAGTVHQGLKTVTPNVVVARSFASPV
                     RFEDISAIGRSKSWCFETHRVTHSHSFFSGDGKRMLCFYQAPDAEAVRLAQREAAMPA
                     EAVWACAAITPQS"
     gene            complement(3248..>4776)
                     /locus_tag="VM692_09860"
     CDS             complement(3248..>4776)
                     /locus_tag="VM692_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52516.1"
                     /translation="RAELAGESGQSLREVLQERLARFDADGGEVLRWAALLAPRIDAA
                     SLARLTGLDWNAIGEALDTAARHSMLVPAERGLRFSHDLIARSIYSGIPPARRRTMHR
                     RVAELLEQDSVLEPERAADLAHHAAQSGDAALAARAMISAGKLCLRFFANDDAVVLAS
                     KGLQWVEHLPGAAERVCLTLELREVMHKAAPVADWETAAREYAALAEQALDLGQLSHA
                     RRGYFMASVVHWMHGHFAGAREEILQSERIARSGNDEEHIVGMAEAARCLAMLERDLT
                     HADAMLMEAQALAARTGVTHNAIPAALGMLRFHENKLEESVELFKQARTLARARGDRL
                     DEFQATEYLAMIDFERDRPEAARVHCKALVELGDKLREGSERPFAHALDALCHYALAD
                     DSGPLDAALDKLRAADAKQRLAYTLTRAALLDLERRRPDAAIARAGEALAYAEALDRA
                     TEIVLAHAVLARARKAVADTAGYARHLAALKSLERAPAAQWARKRASAVAAESSEVRS
                     "
BASE COUNT          721 a         1665 c         1673 g          717 t
ORIGIN      
        1 tcgaggtcgg cgagacgtgg caagcgtcga gcgccgagct cgcgctgaac gggctcgata
       61 agggagcgct cgagcagaca cggacggtga ttcccggtac tgccatcgac gtcgtcctct
      121 cggacttcgc tttgacggag gccgccgtgc cggccaagcc gtttcgctgg cgcatccagg
      181 acgtcggctc gccgcagttc cagctcgagg ttcaggggct cgacgtattg ctcgagcgca
      241 cggcggccgc ggggtatcgg ttcttgagca tcgatgcccg cccgatccaa cggccgttcg
      301 gccgcttcgt attcttgcgc gacgccgacg atattctcgt cgagtacgcg gagcccgctg
      361 cgccgtagtc tcgccactcc cgaatcgttg gcgcggcgtt tgcatgacag gctcgggcct
      421 caacgatgga gtgattcatg gcaggcaaaa tcgatcaagt gaaagggaag atcaacgaag
      481 gaatcggccg gcacaaggag aagaagggcc acgagaatgg cgacccgctc ctcgaggatg
      541 aaggcacggc ccaaaaggtc aaaggcaagc tccagaacgt aaaaggcaaa gtcaaagacg
      601 ctatcgactg agccggcacg tcacggtgcg gacgggcggc cctgcggccg ccccgtccgc
      661 tgttcactta tccgcttcag gcgacgcact gctcacgggg gtgcgcgccg cgcagatgcg
      721 ccaacagatg cgcgctcaag tcacgcgcgt cggcttcggc gccgtgcatg tagctcgatt
      781 tgcggcgccg catgaaattc aggcctagcg tgtagaggcc cggcgccgca acgacgccgc
      841 cctcgtgacg caaccggccg cgcgcgtcga gcacgggcac gtgcagccac gagtagtcgg
      901 gccggaaccc cgtcgcccaa atgatcgtcg cgatcggctc cttggcgaga tcgagcatgc
      961 cgcgcggcga cgttgccacg cgcgtgggcg cgaatcgctc gggtgcgccg acggcgtcgc
     1021 cgatgcggcg ctcggccgcc cagtcgtcga agcgctcgag caaccgcgcg agcttgagat
     1081 cggccatcgc gcactcgttg gtcaacgagc ccgagaacag caggcggctg ccgtcggtgc
     1141 cggcgaggcg tccgacgagc tcgacgcctt gctcgcgcag cgcgttcagg tcgagcgtgc
     1201 gccgttccgg cgaacccaac agctgcggcg aaggcacgcg gcgcgctcgc acgaggtcgt
     1261 cgacctcgtc gtggcgctcg tcgagcacgc cggcggcgtc gagccaccac tgcacgtcac
     1321 ggccgcggta gagccgcggc atgcggatgt gctcaccgac ggcgagcgtg acgggccggc
     1381 cggaccgccg aatctcgtcg gcgagctgca gccccgtggc agaaccgccg acgacgagca
     1441 cgcgcccgaa gtcgagcatg gccgggctgc ggtactcgtt cgcagtcagc gtgcgcacgc
     1501 ttcgcggcag gcgcgccgcg agctccggca cgttcggcac gccgtgcgcc ccgtgtgcca
     1561 gcacgaccgc tcggcagcgc caggtcgcgc ggtccgtctc gacgacgtag ccgtcgccgg
     1621 ccgcgcgcac ggactgtacg gtagtgcccg ttcgcacggg cgctcccgac cgctgcgcgt
     1681 aggccgcgat gaagtcgccg acctcggccg acgtcatgta gccgtccgga tcggggccag
     1741 agtagcgata ccccggtaag cggctttgcc agttcggcgt caggaggcgc agcgagtccc
     1801 agcgctccgt gcgccacgca ttggcaacct cgccgcgctc gagcaccacg tgctcgacgc
     1861 cgcgcgcggc gagcatgcgg ctcatcgcga gacccgaatg gccggcgccg acgacgacgg
     1921 tgtcgacgcc ggccacggcg tgaaggcgat gctgcgggcc ccccttcggg gacttcgaag
     1981 cgtcgcccgc cttcggggac ttcgaagcgg cgcccccctt cggggacttc gaagcgtcgc
     2041 ccatcaggcg acctcgaccg cgacgttcgt cgggttcgtg atgatgtcga acacggccga
     2101 gcggcgctgc gactgcgcga cgagcgcggc gatttccgcg cgcgaagcgt cggcgtcgat
     2161 gcggaacgtc accttgatgc cgtcgaagcc gttgcggacg tcgctgtcga tgccgaggat
     2221 gccctgcatg tccatgccgg cttcgagcgt cgccttgacg gaacgcagct ggatgttgcg
     2281 gttctgcgcc acggaggcga tgccggccgt caggcacgag gcgagcgcca cgagcacgta
     2341 ttcgacgggc gtcgcgccgt tgtcctcggc cgcgaacacc tccggatggt ccgcgtcgta
     2401 gtggaacgcc ttcgggcggg tttgctcggc gccgaggccg aaaaactcct gcaccgtcgt
     2461 gcggctatgc gtgcctctga cccagtcgca cgtcgcgcgc cacttgaagc gcgcggctgc
     2521 cggcgcttcc gtcagcgccg cgcgcgcgcc gatcagggcc tctacgttga cgccattgtc
     2581 gaccttgatt gctgtcacgt tcatgtcttc gctccccgtt aggtttgatc cggccggcgg
     2641 ccggtacggt gagacgatga acgcccgccg ttagaccgag cgtggaagcg cgcgtggaat
     2701 ttccgccgcg aacgtggaat cggcgttggc tagctctgcg gcgtgatcgc cgcgcacgcc
     2761 cagacggcct cggccggcat cgcggcttcg cgctgcgcca ggcgcacggc ttcggcgtcg
     2821 ggcgcttggt agaagcacag catgcgcttg ccgtcgcccg agaagaacga atgcgagtgc
     2881 gtcacgcggt gcgtctcgaa gcaccacgac ttcgagcgcc cgatcgcgga gatgtcctcg
     2941 aaccgcacgg gactcgcgaa cgagcgtgcg acgacgacgt tcggcgtgac ggtcttcagg
     3001 ccttgatgca cggtgccggc ccagaatcgc gatagatcga cgtcggggtc gcgcagcgcg
     3061 aggcgcgccg attccatgtc cgctgccgag aagcggcaga tcaacgcgcg gccgtcgctc
     3121 gcaagaaagc tgccgtgcca agtcacgcga tagaggtcga agcactgcgc cgattcgcgc
     3181 ccgccgtcga cgacgtcctc ggccgtcaac ggctcgtcga acgttcgttc gagaaccaag
     3241 ctcaccgtca tgaccgcacc tcgctcgact ccgccgcgac cgccgacgcc cgcttgcgcg
     3301 cccactgcgc cgcgggcgcc cgttcgagcg actttagcgc agctaggtgc ctcgcgtagc
     3361 ccgccgtgtc ggcgacggcc ttgcgggcgc gcgccagcac ggcgtgcgcg agcacgatct
     3421 ccgtggcgcg gtcgagggcc tcggcatagg cgagcgcctc gccggcccgc gcgatcgcgg
     3481 cgtcgggccg gcgccgctcg aggtcgagca gcgcggcgcg cgtcaacgtg tacgcaagcc
     3541 gctgcttggc gtctgcggca cgcagcttgt cgagcgcggc gtcgagcggc cccgagtcgt
     3601 cggcgagcgc gtagtggcac agcgcgtcga gcgcgtgcgc gaacggccgc tcgctgccct
     3661 cgcgcaactt gtcgccgagc tcgaccagcg ctttgcaatg cacgcgcgca gcctcgggcc
     3721 ggtcacgctc gaagtcgatc atcgccaagt actcggtcgc ttggaactcg tcgagccgat
     3781 cgccgcgtgc gcgcgccagc gtgcgtgctt gcttgaatag ctcgacggac tcctcgagct
     3841 tgttctcgtg gaagcgcagc atgccgagcg cggccgggat cgcgttgtgc gtcacgcccg
     3901 tgcgtgccgc gagagcctgc gcctccatga gcatggcgtc ggcgtgcgtg aggtcgcgct
     3961 cgagcatcgc gaggcaacgc gccgcctcgg ccatgccgac gatgtgctcc tcgtcgttgc
     4021 cgctgcgtgc gatgcgctcc gattgcagga tctcctcgcg cgcgccggcg aagtggccgt
     4081 gcatccaatg aacgacgctc gccatgaagt agccgcggcg cgcatgcgac agctggccca
     4141 ggtcgagcgc ttgctcggcg agcgctgcgt actcgcgcgc cgcggtctcc cagtctgcaa
     4201 cgggcgccgc cttgtgcatg acctcgcgca gctcgagggt caggcagacg cgctcggccg
     4261 cgcccggcaa gtgctcgacc cattgcaacc ctttgctggc gagcacgacc gcgtcgtcgt
     4321 tggcgaagaa gcgcaggcac aactttcccg ccgagatcat ggcgcgtgcc gcgagcgcgg
     4381 cgtcgccgct ctgcgccgcg tggtgcgcga ggtccgctgc gcgctcgggc tcgagcacgc
     4441 tgtcctgctc aaggagctcg gcgacgcggc ggtgcatcgt gcgccgccgt gcgggcggga
     4501 tgccggaata gatgctgcgg gcgatcaaat cgtgcgagaa gcgcaagccg cgctcggccg
     4561 gcacgagcat cgaatgccga gccgccgtgt cgagcgcctc gccgatggcg ttccaatcga
     4621 gccccgtgag ccgcgccagc gaggcggcat cgatccgcgg cgcgagcaag gccgcccagc
     4681 gcagcacctc cccgccgtcg gcgtcgaagc gcgcgaggcg ctcctgcagc acctcgcgca
     4741 gggactggcc gctctccccg gcgagctcgg cgcgcg
//
LOCUS       DATLEE010000457         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_520562, whole genome shotgun sequence.
ACCESSION   DATLEE010000457 DATLEE010000000
VERSION     DATLEE010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520562"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VM692_09865"
     CDS             complement(<1..821)
                     /locus_tag="VM692_09865"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HUQ52517.1"
                     /translation="MTQATGAGGSILSELAQLLASLDPAALDAVTLDAARARIFDTLG
                     ALVVGLQTPEGRLLRSYAERASGEGSALDFMERCRLHVGATRATEIDDIDVASCTTVG
                     AVVVPVALALAARQPRANAGRLLAAVVAGYEAMQRLGRAIGGATLIYRGVWPTYVTAP
                     FAAAATAAKLLGLDAGATRRALELAVTRTAPVPAAALARFGFRYYSLGCAAVDGASAA
                     FAAAAGVDADADALAKFAERAGAALDANELARAAGAPWLLREIDSKLWATSRQALA"
     gene            complement(833..1966)
                     /locus_tag="VM692_09870"
     CDS             complement(833..1966)
                     /locus_tag="VM692_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HUQ52518.1"
                     /translation="MISMEPVLKRGYSSWDRDVLPDDEYALRVEAVRAQLRERGLAAL
                     VVVNYSLLGVMVDYADMAYLSGLQSGGALLIPSDGEPAYVGFGGGRELKFMRTLTWLD
                     HVISGGGPKAFEVVREQLRARGITSGPVAIAGAADLPAGAAARVQQALADYTLQPFDA
                     ELRALRAVKRPREILTMRLTLGLVEDAVYAGLAKFAGGGDNAAAMLEAERVARARKAR
                     DVRVLANMGGPELRPFEGRLDGRHGPLRLWVAAQYQGYWAEAAATSPAPKTSAASKAV
                     AAMLGAARTGATTGEVATAAVAALSPVEAQAALEYGLGGTIGLAHDEGVRIRPGGTER
                     LVEGSVLALRAHVAAGAEPSLDAAVASVGASGATRLQALKIAG"
     gene            complement(1977..>2573)
                     /locus_tag="VM692_09875"
     CDS             complement(1977..>2573)
                     /locus_tag="VM692_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HUQ52519.1"
                     /translation="MREYELAANLEVFMRSLGGEDNFQLMSASQHNTAGHFPTDRVLQ
                     EGDLVLAEITPAVEGEFIQICRSAVLGGPSQLQLEKFALLTHALEAGMRAAKPGVSVT
                     EIVDVINKPIAAAGYEQYTKPPYMRTRGHSMGLGSMEPEVAPGYEHYMQEGMVFVMHP
                     NQYIPETGYIMCGEPVIITKDGAKNLTSRMGSLGTIAL"
BASE COUNT          365 a          970 c          871 g          367 t
ORIGIN      
        1 gcgagcgcct gccgcgacgt cgcccaaagc ttgctgtcga tctcgcgcag cagccacggc
       61 gcgccggccg cgcgcgcgag ctcgttcgcg tcgagcgcgg caccggcgcg ctccgcgaac
      121 ttcgcgagcg cgtcggcatc cgcgtctacg ccggcggccg cggcgaaggc tgcacttgcg
      181 ccatcgacgg ccgcgcagcc gagcgagtaa tagcggaaac caaagcgcgc cagcgccgcg
      241 gccggcacgg gcgccgtgcg cgtcacggcc agctcgagcg cgcgccgagt cgcgccggcg
      301 tctagtccga gcagcttcgc cgcggtggca gccgccgcga acggcgccgt gacgtacgtc
      361 ggccagacgc cgcgatagat caacgtcgcg ccgccgatcg ctcggccgag ccgctgcatc
      421 gcctcgtagc cggcgacgac ggctgcgagc agcctgccgg cattcgcgcg cggttgacgc
      481 gctgccagcg cgagcgccac gggcactacg acggcgccga ccgtcgtgca cgacgcgacg
      541 tcgatgtcgt cgatctcggt cgcgcgcgtg gcgccgacat ggagccggca gcgctccatg
      601 aagtcgagcg cgctgccctc gccgctcgcg cgttccgcgt agcttcgcag cagccgccct
      661 tcgggcgttt gcaatccgac gaccagcgcg ccgagcgtgt cgaagatgcg tgcacgtgcg
      721 gcgtcgagcg tcacggcgtc gagcgcggcg gggtccagcg aagcgagaag ctgcgcgagc
      781 tcgctgagaa tcgaaccgcc cgcgcccgtg gcttgcgtca ttcgctgcga gatcagccgg
      841 cgatcttcag cgcctgcagc cgcgtggcgc cgctcgcgcc gacgctcgcg acggcggcgt
      901 ccagcgacgg ctcggcgccg gcggcgacgt gcgctcgcaa cgccagcacg ctgccttcga
      961 cgaggcgctc cgtcccgccc ggccgaatcc tgacgccttc gtcgtgcgcg agcccgatcg
     1021 tgccgccgag cccgtactcg agcgcggcct gcgcctcgac cggcgatagc gccgccacgg
     1081 ccgcggtcgc cacctcgccc gtcgtcgcgc ccgtgcgagc tgcgccgagc atcgcggcga
     1141 ccgccttgct cgcggcactc gtcttcggcg ccggcgaagt cgccgccgcc tcggcccagt
     1201 agccttgata ctgcgccgcg acccacagcc gcagcggccc gtgccggccg tcgagccgcc
     1261 cttcgaaagg ccgcagctcc ggcccgccca tgtttgcgag cacgcgcacg tcgcgagctt
     1321 tgcgcgcccg cgcgacgcgc tcggcctcga gcatcgcggc cgcgttgtcg ccgccgccgg
     1381 cgaacttcgc gaggccggcg tagacggcgt cttcgacgag cccgagtgtc aagcgcatcg
     1441 taagaatctc gcgcggccgc ttgacggcgc gcagcgcgcg cagctccgca tcgaacggtt
     1501 gcaacgtgta gtcggcgagc gcctgctgca cgcgcgccgc ggcgccggcc ggcagatccg
     1561 ccgcgcccgc gattgcgacg gggccgctcg tgatgccgcg cgcgcgcaat tgctcgcgca
     1621 cgacctcgaa cgcctttggc cctccgcccg agatcacatg atcgagccac gtcagcgtgc
     1681 gcatgaactt gagctcgcgc ccgccgccga aaccgacgta ggccggctcg ccgtcgctcg
     1741 gaatcagcag cgcgccgccg gactgcagcc ccgagagata cgccatgtcg gcatagtcga
     1801 ccatgacgcc gagcaagctg taattgacga cgacgagcgc cgcgagcccg cgttcgcgga
     1861 gctgcgctcg cacagcctca acgcgcagcg cgtactcgtc gtcgggcagc acgtcgcgat
     1921 cccacgacga gtagccgcgc ttcaaaaccg gttccatgct gatcatcggc ggcccctcaa
     1981 agcgcgatcg tgccgagcga gcccatgcgg ctcgtcagat tcttggcgcc gtccttcgtg
     2041 atgatgacgg gctcgccgca catgatgtag cccgtctccg ggatgtactg gttcgggtgc
     2101 atgacgaaca ccatgccctc ttgcatgtag tgctcgtacc ccggagcgac ctcgggctcc
     2161 atcgagccca agcccatgct gtggccgcgc gtgcgcatgt acggcggctt cgtgtactgc
     2221 tcgtagcccg cggccgcgat cggcttgttg atgacgtcga cgatctcggt caccgagacg
     2281 ccgggcttcg cggcccgcat gccggcctcg agcgcgtgcg tcagcagcgc gaacttctcg
     2341 agctgcagct gcgacgggcc gccgagcacg gcgctgcggc agatctgaat gaattcgccc
     2401 tcgacggccg gtgtgatctc cgcgagcacg agatcgccct cctgcaacac gcgatccgtc
     2461 gggaaatgcc cggccgtgtt gtgctgcgac gccgacatca gctggaagtt gtcctcgccg
     2521 cccaagctgc gcatgaacac ctcgaggttc gccgcgagct cgtactcgcg cat
//
LOCUS       DATLEE010000458         5869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2006036, whole genome shotgun sequence.
ACCESSION   DATLEE010000458 DATLEE010000000
VERSION     DATLEE010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006036"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VM692_09880"
     CDS             complement(<1..193)
                     /locus_tag="VM692_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HUQ52520.1"
                     /translation="MARFDLVIFDCDGVVVDSERIVFEVFDVFVRSQGVDLTETETRE
                     RFLGRTLAACVAIIEKLSGK"
     gene            complement(193..621)
                     /gene="arfB"
                     /locus_tag="VM692_09885"
     CDS             complement(193..621)
                     /gene="arfB"
                     /locus_tag="VM692_09885"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HUQ52521.1"
                     /translation="MAEGGLEINGRVTIPEAELEFAAIRSQGAGGQNVNKVASAVQLR
                     FDAARSSALPAGARERLLAQRDHRISDDGVVVIKAQQYRSQEQNKAAALERLAELVRR
                     ALVVPKVRRATKPTRASKMRRLDDKKKHSRLKQRRRTLDD"
     gene            complement(631..1869)
                     /locus_tag="VM692_09890"
     CDS             complement(631..1869)
                     /locus_tag="VM692_09890"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ52522.1"
                     /translation="MLSRQLVSCVAAAAFVLGVSPLAAQQQPQQRAPAAGNAVGATPR
                     PPTGIVVPPLGAGPFGYQTAEEKIRVVVHTRGLKRPWSLVWLPSGEMLVTERGGQLRI
                     VRNGELDPQPVAGLPEIRAQGLSGLYDVALHPQFATNKLVYLSYQKPIAERQNALAVA
                     RGVFNGRALTDVKEIFATTDSSSVSRLAFGRDGKLYVSTFGNAGDGSGAQDPMSDAGK
                     VLRLNDDGSVPSDNPFSGKPGYKPEIYTLGHRSTLALIVHPVTGELWENENGPNGGDE
                     INVLKPGANYGWPLVSTGRSYPGPWQSKGFSRDGFEDPLVYWVPSIAVSGLAIYTGDK
                     FPRWKGDAFVGGMRMGEIPGTGHMQRIHFNNNNEELRREQMLVDLRQRIRDVRQGPDE
                     LLYLLTDEDNGAILRIEPAN"
     gene            2031..2459
                     /locus_tag="VM692_09895"
     CDS             2031..2459
                     /locus_tag="VM692_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUQ52523.1"
                     /translation="MPTAHDILRFKGSAIHCVRPDDTVLAALGVMAEHDIGAVLVLDG
                     DELVGILSERDYARKVVLVGRTSKDSPVRAIMTADVVCVPPERSIEDCMALMTQHRMR
                     HLPVLDNGQVIGLVSIGDLVKATIDEQEFTINQLKNYIAG"
     gene            2557..3129
                     /locus_tag="VM692_09900"
     CDS             2557..3129
                     /locus_tag="VM692_09900"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HUQ52524.1"
                     /translation="MKKLFLAGLASVAVALPVAAALTKGDKAPTFTAQASLAGKEFKY
                     SMADALKKGAVVVYFYPSAFTQGCNIQAHTFAENMDAFTAAKATVIGVSLDSIQRLND
                     FSADPAYCAGKLAVASDADGSIAKSYGVNVREGRAGMQDSRGVAIDHGFADRLTFIVA
                     KDGTVAATVGGQGVSPEQNVEQALQAVKAL"
     gene            3226..4125
                     /locus_tag="VM692_09905"
     CDS             3226..4125
                     /locus_tag="VM692_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HUQ52525.1"
                     /translation="MPTRTPLLVTGAAGNLGRQVVEQLLETNAGPIIATTRTPEKLAD
                     FAKRGVDVRAADFNDLQSLVDAFRGAERLLLISTGDLFPEGARLRQHRAAVQAAVDAG
                     VKHVVYTSGPAPHPTPKGALINDHYWTEQALAASPLEWTILRHHIYTDFLVGTLAQAL
                     KFGVLSGSAGDGGNNYVTRADCARADAAALAADFSGRRILDVTGPAPVTPGELVNIAS
                     ALAGKPLRYQHLTRAEHEKMLLGFNLPPFLVDALASFDEAQSQGHLAIRSPAVFNLTG
                     KAPTSVQEYLTAQRPALLAAASS"
     gene            4256..4927
                     /locus_tag="VM692_09910"
     CDS             4256..4927
                     /locus_tag="VM692_09910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ52526.1"
                     /translation="MRSIALAVLLGALCAAAVDPAAAAVERAGAAVQALLDSARQELS
                     AGRAQQASTLLERALRIEPQNPTVWHYLGRARLELGDYAQAAAMATKSHSLTGNDRTL
                     RVGNAELLSSALQSSGRAATDIERQVLAAPSTFDWGVERARAYVGTTEEPSEDQARRG
                     WSLPETARQTWRDAQASTWRQAQASAQRAQAAAERAEAAARRAEEAYRRYYESNDGVR
                     RRRQY"
     gene            complement(4924..5805)
                     /locus_tag="VM692_09915"
     CDS             complement(4924..5805)
                     /locus_tag="VM692_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HUQ52527.1"
                     /translation="MLDNRLLDGRAVRDRILDAVAARVHAGTAKHKLGRLVSISIGEH
                     KEVAVYVRGQAGAAKKVGIPFEDQTWPATLTQDECKARLRAMNDDSTVLGVILQRPIP
                     KHLHGRSLASAIHPLKDIEGMNPASIGDIVYNDAALAPCTAAASVELIKATGLNLHGL
                     EVVMIGHSEIVGKPAAFMLMAEGATVTVCHHLTRSVAAHSRRADAVLVAVGKPRFLRA
                     DMVKPGAAVIDIGINQIAGPNGETLIVGDVDTENVKEVASWVTPVPGGVGPVTVAMFM
                     RNAITAHEKQFAAGWLR"
BASE COUNT          966 a         2008 c         1896 g          999 t
ORIGIN      
        1 gcttgccgct cagcttctcg atgatcgcga cgcacgcggc cagcgtgcgg ccgagaaaac
       61 gctcgcgtgt ttctgtttcg gtcagatcga cgccttgcga gcgaacgaat acatcgaaca
      121 cctcgaagac gatgcgctcg ctgtctacga cgacgccgtc gcagtcgaaa atgacgaggt
      181 cgaagcgtgc catcagtcgt cgagcgtgcg tcgccgctgt ttgaggcgcg aatgtttctt
      241 cttgtcgtcg aggcgcctca tcttcgacgc gcgcgtcggc ttcgtcgcgc gccgcacttt
      301 cggcacgacc agcgcgcggc gcacgagctc ggccaggcgc tcgagcgccg cggccttgtt
      361 ctgctcttgg ctgcgatatt gctgagcctt gatcacgacg acgccgtcgt cggagatgcg
      421 atggtcgcgt tgcgcgagca accgctcgcg agcgccggcc ggcagcgcgc tcgagcgcgc
      481 cgcgtcgaag cgcagctgca cggcgctcgc gaccttgttg acgttctgcc cgcccgcacc
      541 ctgcgaccta atcgcggcga actcgagctc ggcctcgggg atcgtcacgc ggccgttgat
      601 ctcgagcccg ccttcagcca tgccgcatgc tcagttcgcg ggctcgatgc gcaagatcgc
      661 gccgttgtcc tcgtcggtca gcagatacaa gagctcgtcg gggccttgcc gcacgtcgcg
      721 tatgcgctga cgcagatcga cgagcatttg ctcgcggcgc agctcctcgt tgttgttgtt
      781 gaagtggatg cgctgcatgt ggcccgtgcc cggtatctcg cccatgcgca tgccgccgac
      841 gaacgcgtcg cccttccagc gcgggaactt gtcgccggta taaatcgcaa gaccggacac
      901 ggcgatcgac ggcacccaat agacgagggg atcctcgaag ccgtcgcgtg agaagccttt
      961 cgactgccac ggccccggat agctgcggcc cgtgctgacg agcggccacc cgtagttggc
     1021 tccgggcttc agcacgttga tctcgtcgcc gccgttcggg ccgttctcgt tctcccagag
     1081 ctcgcccgtc acgggatgca cgatcaacgc cagcgtgctg cgatggccca gcgtgtagat
     1141 ctcgggcttg tagccgggct tgcccgagaa cggattgtcg ctcggcacgc tgccgtcgtc
     1201 gttcaagcgc agcaccttgc cggcgtcgct catggggtct tgcgcgccgc tgccgtcgcc
     1261 ggcgttgccg aacgtgctga cgtagagctt gccgtcgcgg ccgaacgcga gccgcgagac
     1321 gctgctcgag tccgtcgtgg cgaaaatctc tttcacgtcg gtcagcgcgc gaccgttgaa
     1381 gacgccgcgc gcgacggcca aagcgttttg ccgctcggca atcggctttt gatagctgag
     1441 atagacgagc ttgttggtcg cgaactgcgg atgcagcgcg acgtcgtata gccccgacaa
     1501 cccttgcgca cgaatctcgg gcagccccgc gaccggctgc ggatcgagct cgccgttgcg
     1561 cacgatgcgc agctgaccgc cgcgttcggt cacgagcatc tcgccactcg gcagccagac
     1621 gaggctccac gggcgcttga ggccgcgcgt gtgcacgacg acgcgaattt tttcctcggc
     1681 cgtctggtat ccgaacggcc ccgctccgag cggcggcacg acgatacccg tcggcggccg
     1741 cggcgttgct cccacggcat tgccggccgc cggcgcgcgc tgttgtggtt gctgttgcgc
     1801 cgcgagcggc gacacgccca gaacgaacgc cgctgcggcg acgcaactga cgagctgacg
     1861 gctaagcatg ctctcccccc gagacgtagc gcctgaaaga cagggcatgg taacgcgcag
     1921 cgctacgact tgtccgtagg cgccgggctc gcgccgctcg cacgcagcgc gtaatcgggg
     1981 cacacttggg tctcggtgat cgactcgttc aactttccgg agaacactcg atgcctaccg
     2041 ctcatgacat tctgcgcttc aagggcagcg ccattcactg cgttcgcccc gacgacaccg
     2101 tgctcgccgc gttaggcgtc atggccgagc acgatatcgg ggcagtgctc gtcctcgacg
     2161 gcgacgagct cgtcggcatc ctcagcgagc gcgactacgc acgtaaggtg gtgctcgtag
     2221 gccggacgtc gaaggactcg ccggtcaggg ccatcatgac agccgacgtc gtttgcgtgc
     2281 cgccggagcg ctcgatcgag gactgcatgg cgctgatgac gcagcaccgt atgcggcact
     2341 tgcccgtgct cgacaacggc caggtgattg gcctggtttc gatcggcgac ctcgtcaaag
     2401 cgacgatcga cgagcaggaa ttcaccatta atcagctcaa gaactacata gccggctgac
     2461 taggccgcct cgcgcccctt gcgcgcagcc gtaagccttg tcacgatttc gtcaggcctt
     2521 cggccgaatc cacacatacc ttcgttgggg gagcccatga aaaaactatt tctcgctggc
     2581 ttggcatccg tcgctgtcgc actgcccgtc gccgccgctc tgacgaaggg cgacaaagcg
     2641 cccacgttca cggcgcaagc gtcgctggcc ggtaaggagt tcaagtactc gatggccgac
     2701 gcgctcaaaa aaggcgccgt cgtcgtctac ttctatccgt cggctttcac gcaaggctgc
     2761 aacatccagg cgcacacgtt tgcggagaac atggacgcct tcacggcggc caaggccacc
     2821 gtcatcggcg tgtcgctcga cagcattcag cgcttgaacg atttctcggc cgacccggcc
     2881 tactgcgcgg gcaagctcgc cgtcgcatcg gacgccgacg gctcgatcgc gaagtcgtat
     2941 ggagtcaacg tgcgtgaagg ccgcgccggc atgcaggatt cgcgcggcgt cgctatcgac
     3001 cacggcttcg ccgaccgctt gacgtttatc gtcgcgaagg acggcacggt cgcggccacg
     3061 gtcggcggcc agggcgtctc gcccgaacag aacgtcgagc aggcgttgca agcagtgaaa
     3121 gcgctctaac agcgctccga tcttcgttcc accaacggag cccgttgcgc agccgcgcaa
     3181 cgggctccgt ctttttgctg actatcgaac ccgcgagact tcacgatgcc aacccgcacg
     3241 cctctgttgg tgaccggcgc cgccggtaat ctcggccgcc aagtcgtcga gcaactgctc
     3301 gagaccaacg ccgggccgat catcgccacg actcgcacgc ccgagaagct cgcggatttc
     3361 gcgaagcgcg gcgtcgacgt gcgagcggcc gacttcaatg acttgcagag cctcgtcgac
     3421 gcgttccgcg gcgccgagcg cttactgttg atcagcacgg gcgacctgtt tcccgaaggc
     3481 gcgcggctgc gccagcaccg cgccgcggtg caagcggccg tcgacgccgg cgtcaagcac
     3541 gtcgtctaca cgtcgggccc ggcgccgcat ccgacaccta aaggcgcgtt gatcaacgac
     3601 cattactgga ccgagcaagc gctcgccgcg agcccgctcg agtggacgat tctgcgccat
     3661 cacatctaca cggactttct cgtcggcacg ctcgcgcaag cgttgaagtt cggcgtgctg
     3721 tccgggtcgg ccggcgacgg cggcaacaac tacgtgacgc gtgcggattg cgcgcgcgcc
     3781 gacgcggcgg cgctcgcggc ggacttcagc ggccggcgca ttctcgacgt gacagggccc
     3841 gcgcccgtca cgccgggcga gctcgtcaac atcgcgagcg cgctcgccgg caagccgctg
     3901 cgctaccagc atctgactcg ggccgagcac gagaagatgc tgctcggctt caacctgccg
     3961 ccgttcctcg tcgacgctct ggcctcgttc gacgaagcgc aatcgcaggg gcacctcgcg
     4021 attcgctcgc cggccgtgtt caacctcacg ggcaaggcgc cgacgtccgt gcaggagtac
     4081 ctgacggcac aacgcccggc gttgctggcc gcagcctcga gctagcgaag gcggcgcgcg
     4141 atgcgccggc acgccaaatc gtcgcaacga ttggtgcttg ctgccgggtc gagggcggat
     4201 taaggtggtt tctcaggcgc tctgcctaac cccacggaac gcctcacgga gctcgatgag
     4261 atccattgcc ctagccgtgc ttctcggcgc gctttgcgcg gccgcggtgg accccgcggc
     4321 ggcggctgtc gagcgcgccg gcgcagccgt gcaagcgctg ctcgacagcg cccggcaaga
     4381 gctcagcgcg gggcgggcgc aacaagcctc gacgctgctc gagcgcgcgc tgcgcatcga
     4441 gccgcaaaac cccaccgtct ggcactacct tgggcgggcg cgactcgagc tcggcgacta
     4501 cgcccaagcg gccgccatgg ctaccaaatc gcacagcttg accggcaacg atcgcacgct
     4561 gcgagtcggc aacgccgagc tgctgtcgtc ggcgctgcaa tcgtcggggc gcgccgcgac
     4621 cgacatcgag cgccaggtcc tggctgcgcc gagcacgttc gactggggcg tcgagcgtgc
     4681 gcgcgcgtat gtgggcacga ccgaagagcc gagcgaagat caggcgcgcc gaggctggtc
     4741 gctgcccgag accgcgcggc aaacgtggcg cgatgcgcaa gcgtcgacct ggcgacaagc
     4801 ccaagcgtcg gcgcaacgag cgcaggctgc cgccgagcga gccgaggccg ccgcaaggcg
     4861 cgccgaagag gcgtaccggc gctattacga atcgaacgac ggcgtgcgcc gccggcggca
     4921 gtactagcgc agccagcccg ccgcgaactg cttttcgtgc gccgtgattg cgttacgcat
     4981 gaacatcgcg acggtcacgg gccctactcc gccgggcacg ggcgttaccc agcttgcgac
     5041 ctctttcacg ttctcggtgt cgacgtcacc cacgatcagc gtttccccgt tcggacccgc
     5101 gatctgattg atgccgatgt cgatgacggc ggcgcccggc ttcaccatgt cggcgcgcag
     5161 gaagcgtggc tttccgaccg cgaccagcac agcgtcggca cggcgcgagt gcgcggcgac
     5221 cgagcgcgtc aggtgatgac agacggtcac cgtcgccccc tcggccatca acatgaacgc
     5281 cgcgggctta ccgacgatct ccgagtgccc gatcatgacg acctcgagcc cgtgcaagtt
     5341 caacccggtt gccttgatga gctcgacgga tgccgccgcc gtgcacggcg ccagtgctgc
     5401 gtcgttgtag acgatgtcgc cgatcgaggc ggggttcatg ccttcgatgt ctttcagcgg
     5461 atgaattgcc gacgccaacg atcggccatg taaatgcttc gggatcggcc gttgcagaat
     5521 gacgccgagc accgtcgagt cgtcgttcat cgcgcgcagc cgcgctttgc actcgtcctg
     5581 cgtcagcgtc gcgggccacg tctgatcctc gaacggaatg ccgactttct tcgcggcgcc
     5641 cgcttgcccg cgcacgtaaa cggcgacctc cttgtgctcg ccaatgctga tcgacacgag
     5701 ccggccgagc ttgtgcttgg ccgtgccggc atgcacgcgc gcggcgacgg cgtccagaat
     5761 acggtcgcga acggccctgc cgtccagaag gcggttgtcg agcatggatg cgttccggta
     5821 tggactaatt aaaggtgttg cacgcggact gattgccgcc gcgaagacc
//
LOCUS       DATLEE010000459         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1262926, whole genome shotgun sequence.
ACCESSION   DATLEE010000459 DATLEE010000000
VERSION     DATLEE010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262926"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VM692_09920"
     CDS             complement(<1..199)
                     /locus_tag="VM692_09920"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HUQ52528.1"
                     /translation="MDRSGTQRLRLRVELAAQTLTLHGGGRPLKTYAVSTSKHGAGEI
                     SGSFKTPRGRHVVRAKIGAGAP"
     gene            complement(172..657)
                     /locus_tag="VM692_09925"
     CDS             complement(172..657)
                     /locus_tag="VM692_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HUQ52529.1"
                     /translation="MPREIERKFLLQNDDWRALAQRRVRMSQGYLASGGRVSVRIRIA
                     GDVAHLNMKAGGLVASRAEYEYPVPVADARELLALADGPLIDKTRHFVEHGGFEWEVD
                     EFHGDNSGLIVAELELAREDEPFPRPPWLAKEVTQLERYYNVRLVKHPYRAWTEAERS
                     A"
     gene            complement(685..1986)
                     /gene="rlmD"
                     /locus_tag="VM692_09930"
     CDS             complement(685..1986)
                     /gene="rlmD"
                     /locus_tag="VM692_09930"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HUQ52530.1"
                     /translation="MRVDGAAVVATIVDLTHDGVGVADLDGRKVFVADALPGERVELV
                     LRKRRRKLQEADLVRVLEPSPARVQPACEYFGRCGGCALQHLAHAEQVTFKQRVVAET
                     LKRIGRVEPEEWLPAVVSEPWHYRRRARLGVKYVAGKGRVLVGFRERAGPYIMDMRHC
                     PVLKPPIGGLLAELAELIEHSSLKQQLPQIEAAVADDAVALVLRVLADPSPADVEAFR
                     AFGARHDIDLYWQRGGPGTVVPLDRARPLRYRLPELDLTYEFLPTDFVQVNAGINAEL
                     VATALRLAAVEPTDRVLDLYCGLGNFSLPFARRAAELTGIEGDAGLVARAVRNAAANG
                     INNARFLTADLMAVGWSFYREHWDIVVLDPPRTGAETPVAELTGLGPRRIVYVSCHPA
                     TLARDAQVLVERHGFKLRAARVFDMFPHTHHIEALALFTRD"
     gene            complement(1976..>2195)
                     /locus_tag="VM692_09935"
     CDS             complement(1976..>2195)
                     /locus_tag="VM692_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HUQ52531.1"
                     /translation="FRRGEVAAIRDYCEIDVLNTYLIYLRFELTRGELDAATYQKELE
                     LVEAKLAQSDRAHLKQYLELWQTSRHAG"
BASE COUNT          368 a          745 c          749 g          333 t
ORIGIN      
        1 acggcgcgcc ggcgccgatc tttgcgcgga cgacgtgccg gccgcgcggc gtcttgaagc
       61 tgccgctaat ctcgccggcg ccgtgcttcg acgtcgacac ggcgtaagtc ttcagcggcc
      121 gaccgccgcc atgaagcgtc aacgtctgtg ccgccagctc gacgcgcaac ctcaggcgct
      181 gcgttccgct tcggtccatg cgcggtacgg atgcttgacg agacggacgt tgtaatagcg
      241 ctcgagctga gtgacttcct ttgctagcca cggcgggcgc gggaacggct cgtcctcgcg
      301 agccagctcg agctcggcca cgatcaaccc tgagttatcg ccgtgaaact cgtcgacctc
      361 ccactcgaaa ccgccgtgct cgacgaagtg gcgcgtcttg tcgatcaacg gaccgtcggc
      421 gagggcgagc agctcgcgcg cgtcggcaac gggcacggga tactcgtact cggcgcgaga
      481 agcgacgaga ccgccggcct tcatgttgag gtgcgccacg tcgccggcga tgcggatgcg
      541 cacggacacg cggccgccgc tcgcgagata accctggctc atgcgcacac ggcgttgggc
      601 gagcgcgcgc cagtcgtcgt tctgcaagag gaatttgcgc tcgatttccc gcggcatggc
      661 gcgatgatag ccgcgatctc gacgtcagtc gcgcgtgaac agcgccagcg cttcgatgtg
      721 atgagtgtgc gggaacatgt cgaataccct cgcggcccgc agcttgaaac catgccgctc
      781 gacgagcact tgcgcatcgc gcgcgagcgt cgccggatgg cacgacacgt agacgatgcg
      841 gcgcggcccg aggcccgtga gctcggctac gggcgtctcg gcgcccgtgc gcggcggatc
      901 gagcacgacg atgtcccagt gctcgcggta gaagctccaa cccacggcca tgaggtcggc
      961 cgtcaaaaaa cgcgcgttgt tgatgccgtt ggcggccgcg ttgcggacgg cgcgcgcgac
     1021 gaggccggcg tcgccctcga tgcccgtgag ctcggccgcg cgccgcgcga acgggaggct
     1081 gaaattgccg agcccgcaat acaaatcgag gacgcgatcc gtcggctcga ccgcagccaa
     1141 acgcagcgcc gtcgcaacga gctcggcatt gatgccggca ttgacctgca cgaaatcggt
     1201 cggcaggaac tcgtaggtga gatcgagctc gggtagccga tagcgcagcg gccgcgcgcg
     1261 atcgagcggc actacggtgc cggggccgcc gcgctgccag tagaggtcga tgtcgtgccg
     1321 cgcgccgaac gcgcggaacg cctcgacgtc ggcggggctg gggtcagcca gcacgcgcag
     1381 cacgagcgcc acggcatcgt cggccacggc cgcttcgatc tgcggcagct gctgcttgag
     1441 actgctgtgc tcgatgagct ccgcgagctc ggcgagcaag ccgccgatcg gcggcttcaa
     1501 cacgggacag tggcgcatgt ccatgatgta cgggcccgcg cgctcgcgaa agccgacgag
     1561 cacgcggccc ttgccggcca cgtatttgac gccgaggcgc gcgcggcgcc gatagtgcca
     1621 cggctcgctg acgacggcgg gcaaccactc ctcgggctcg acgcgcccga tgcgtttcag
     1681 cgtttcggcg acgacgcgct gcttgaacgt gacctgctcc gcgtgcgcga gatgctgcag
     1741 cgcgcagccg ccgcagcggc cgaaatattc gcacgccggc tgcacgcgcg ccggcgacgg
     1801 ctcgagcacg cggacgagat cggcttcctg cagcttgcgg cggcgcttgc gcagcacgag
     1861 ctcgacgcgc tcgcccggca gcgcgtccgc gacgaagact ttgcggccgt cgagatcggc
     1921 gacgcccacg ccgtcatgcg tcagatcgac gatcgtggca acgactgcgg caccgtcaac
     1981 ccgcatgccg cgaagtctgc caaagctcaa gatactgctt cagatgcgcc cggtccgact
     2041 gcgcgagctt cgcttcgacg agctcgagct ccttttgata cgtggccgca tcgagctcgc
     2101 cgcgcgtgag ctcgaagcgc aagtagatca agtaggtgtt caggacatcg atctcgcagt
     2161 agtcgcgaat cgcggctacc tcaccgcggc gaaac
//
LOCUS       DATLEE010000460         2193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_523784, whole genome shotgun sequence.
ACCESSION   DATLEE010000460 DATLEE010000000
VERSION     DATLEE010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523784"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2193
                     /locus_tag="VM692_09940"
     CDS             <1..>2193
                     /locus_tag="VM692_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ52532.1"
                     /translation="VREVERLREDLHNLLSSGEGADRIAVNGRLSSFVDISASMNRLL
                     DRSDESARLAAQAPEPPRAGERNELFEALAATLPEVALIHTNTILYANRAAGELFGVA
                     PEALVGKPITDLVRPAYRAVMRKHVGPDADDPLTPFEVQLISNDDQGMWAELHSRRLE
                     FGGEHALLTVARDITHRKSLETSLGRGKLQARITLESIGEGVITTDRNGTIDYMNEAA
                     EQLTGGTRSAGIGKRLLDLLSLVDEIDRSSLGDPVAKCLSERRRVNLGRRAFLLSKQA
                     EREFSTELTASPIRGPDGQVAGCVVIFHDVSELRGLAREMSYQASHDALTGLVNRAEF
                     ERRLEAALDSARGEGVGHVVCYLDLDRFKLVNDTGGHIAGDNLLREIAALLKQRVRDS
                     DTVARVGGDEFAMLLAGCPLDKARQIADDVCQSVAGHQFAWQDSIFDVGVSVGLVEVG
                     KESGSAESVLSAADSACYIAKQQGRGRTHVYSTRDEVMARERGEIQWLQRLQRALKEN
                     GFELYVQPIVAIGGRNRAGPAAEVLLRMRDESGSVILPVHFLASAERYQLMSHIDRWV
                     VQTTLAAIAGGAPHLPAGRTCNINLSAQTLGDDDFLEFVVDVLDHTGVSPDRICFEVR
                     ESAVVSQLDQAQRFINVLHGIGCRFALDNFGSGIGSFANLKNLSLDYLKIDGTYTRNL
                     ETDGVNREMVAAMVKLARTLDFQVVAEQVEDQSSFEALRALGVDFVQGF"
BASE COUNT          367 a          732 c          749 g          345 t
ORIGIN      
        1 gtgcgcgagg tcgagcggct gcgcgaagac ttgcacaatc ttttgtcctc cggcgaaggc
       61 gccgaccgta tcgcagtcaa cggccggctc agctcattcg tcgacatctc ggcgtcgatg
      121 aaccgcttgc tcgatcgcag cgacgagtcg gcgcgcttgg ccgcgcaagc gccggagccg
      181 ccgcgcgccg gcgagcgcaa cgagctgttc gaagcgctgg ccgcgacgct acccgaggtc
      241 gccctgattc acacgaacac gatcttgtac gccaatcgcg cggccggcga gctgttcggc
      301 gtcgcgcccg aggcgctggt cggcaagccg atcacggatc tcgtgcggcc cgcgtatcgt
      361 gccgtgatgc gcaagcatgt cggccccgac gccgacgatc cgctgacgcc gttcgaggtg
      421 cagctcatca gcaacgacga tcaaggcatg tgggccgagc tccatagccg gcgcctcgag
      481 ttcggcggcg agcatgcgct cctgacggtc gcgcgcgaca tcacgcatcg caagagcctc
      541 gagacgtcgc tcggccgcgg gaagctgcag gcgcgcatca cgctcgaatc gatcggcgag
      601 ggcgtcatca cgacggatcg caacggcacg atcgactaca tgaacgaggc ggccgagcag
      661 ctgaccggcg gcacgcgcag cgccggcatc ggcaagcggc tgctcgatct cctgtcgctc
      721 gtcgacgaga tcgatcgcag ctcgctcggc gaccccgtcg cgaagtgctt gagcgagcgg
      781 cggcgcgtga acttgggcag gcgagcgttc ctgttgagca agcaagccga gcgcgagttc
      841 tcgacggagc tgacggcgtc gccgatccgc gggcccgacg gccaagtcgc gggctgcgtc
      901 gtgatcttcc acgacgtcag cgagctgcgc gggttggcgc gcgagatgtc gtaccaggcg
      961 agccacgacg cgctgacggg gctcgtcaac cgcgcggagt tcgagcggcg cctcgaggca
     1021 gcactcgact ccgcgcgcgg cgaaggggtc gggcacgtcg tctgctacct cgacctcgat
     1081 cgattcaagc tcgtcaacga tacgggcggg cacatcgccg gcgataactt gctgcgcgag
     1141 atcgccgcgc tgctgaagca acgcgttcgc gattcggata ccgtcgcgcg cgtcggcggc
     1201 gacgagttcg caatgctgct cgcgggctgc cctctcgaca aggcacggca gatcgccgac
     1261 gacgtttgcc agtcggtggc gggccatcaa ttcgcgtggc aggacagcat cttcgacgtc
     1321 ggcgtcagcg tcggtctcgt cgaggtcggc aaggagagcg gctcggccga aagcgtcctg
     1381 agcgccgccg actccgcgtg ttacatcgcg aagcagcagg gcagaggccg cacgcacgtc
     1441 tactcgacac gcgacgaggt catggcgcgc gagcgcggcg agatccagtg gctgcagcgg
     1501 ctgcagcgcg cgctcaagga aaacggcttc gagctctacg tgcagccgat cgtcgcgatc
     1561 ggcgggcgca accgcgcggg cccggcggcc gaagtgctgc tgcgcatgcg cgacgagtcc
     1621 ggtagcgtga tcctgcccgt gcacttcctg gcttcggccg agcgttatca gctgatgtcg
     1681 cacatcgacc gctgggtcgt gcaaacgacg ctggccgcga tcgcgggcgg cgcaccgcac
     1741 ctgccggcgg gccgcacttg caacatcaac ctgtcggcgc agacgctcgg cgacgacgac
     1801 ttcctcgagt tcgtcgtcga cgtgctcgac catacggggg tcagcccgga ccgcatctgc
     1861 ttcgaggtgc gtgagtcggc cgtcgtcagt cagctcgacc aggcgcagcg cttcatcaac
     1921 gtgctgcacg gcatcggttg ccgcttcgcg ctcgacaact ttggcagcgg catcggctcg
     1981 ttcgccaacc tgaaaaatct ctcgctcgac tatttgaaga tcgacggcac ctatacgcgc
     2041 aacctcgaaa ccgacggcgt caaccgcgag atggtggcgg cgatggtcaa gctggctcgc
     2101 acgctcgact ttcaagtcgt ggcggagcaa gtcgaggacc agagctcgtt cgaggcgctg
     2161 cgcgcgctcg gcgtcgactt cgtccagggc ttc
//
LOCUS       DATLEE010000461         3052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2756096, whole genome shotgun sequence.
ACCESSION   DATLEE010000461 DATLEE010000000
VERSION     DATLEE010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2756096"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VM692_09945"
     CDS             complement(<1..453)
                     /locus_tag="VM692_09945"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HUQ52533.1"
                     /translation="MAVKIVLELDDKDLDYYRGVMDSIWKNAQKRSEKELMEGSRRLL
                     KQATKAGAPEYVRSRLAELEALCAMLDDSEWPLEDDDRNRIRAALGYFALAKDMIPDK
                     IPGLGFLDDALMADLVTRELKLELEGYEAFCEYRDNETKLRGKAKVSRL"
     gene            complement(648..890)
                     /locus_tag="VM692_09950"
     CDS             complement(648..890)
                     /locus_tag="VM692_09950"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ52534.1"
                     /translation="MLLLPALAEFNRRYLQAINDGDIETLASLTTEGHMMISSGGPPL
                     TGKKALVDAMTRAFATTDFDESWAPEETVVSGDLAY"
     gene            1311..1853
                     /locus_tag="VM692_09955"
     CDS             1311..1853
                     /locus_tag="VM692_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUQ52535.1"
                     /translation="MADEPESAAVEDPRAAFVTGVFLRFQHSLLRYLRDLLSRREDAE
                     DVAQETYLKLVHAGGLEHSEVRVRAFMFKVATNLAYDRFRQRRSRGRQDDSELVALPD
                     DAPAADRVVALEQGLSIVERTLLGLPARCRQVFLLRVAHEWSYEAIAERLEVSKRTVE
                     REMQTALEACERNHKKGELP"
     gene            1850..2728
                     /locus_tag="VM692_09960"
     CDS             1850..2728
                     /locus_tag="VM692_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52536.1"
                     /translation="MNRTDATEANDEAAEAARRFVRADSGSEPANDAELASWLAARPA
                     NERAMQRIDLAVELGRRLAAEPSSALHAEAQLAARARPRPYRTPLLRTLAWGGALAAL
                     LLVALFVVRDDVVPPEPIVIQAARTVASAAPSSAVAVLPSGVLVDASAVAVLPFAGAR
                     DAALAAGLEHDLVQALRTVPGLYVIADAAVQPYARTELDAAELGGLLGARGLVDGSVE
                     LAGGRVRVSARLREAATGATLWQAELDRPVDELRAVRYEIAEQVATAMFDSGARAALA
                     PPERDDETFSGKPFQQ"
     gene            2770..>3052
                     /locus_tag="VM692_09965"
     CDS             2770..>3052
                     /locus_tag="VM692_09965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ52537.1"
                     /translation="MRIAHIEAGRHLYGGAAQVRYLIEGLTGRGVENLLFCPPGSDLG
                     AALPAQQVVELPMHGELDVTLLPRLARTLKRTQPDLVHVHSRRGADLYGG"
BASE COUNT          490 a          990 c         1057 g          515 t
ORIGIN      
        1 gaggcggctg accttcgcct tgccgcgcag cttggtctcg ttgtcgcggt actcgcagaa
       61 cgcttcatag ccttcgagct caagcttgag ctcgcgtgtc acgagatcgg ccatcagcgc
      121 gtcgtcgagg aagcccaagc cggggatctt gtcgggaatc atgtccttcg cgagcgcgaa
      181 gtagccgagc gcagcgcgaa tgcgattgcg atcgtcgtcc tcgagcggcc actccgagtc
      241 gtcgagcatc gcgcatagtg cctcgagctc cgccaagcgg ctgcgcacgt actcgggggc
      301 tcccgccttc gtcgcctgct tcagcagccg tcgcgaacct tccatgagct ctttttcaga
      361 tcgcttctgg gcgttcttcc agatcgagtc catgacgccg cggtagtagt cgaggtcttt
      421 gtcgtcgagc tcaagaacga ttttcacggc cattgttgtt atcccccgct tgagcgaacg
      481 cagactaccg atcacgactg cagatgccaa gtcccgcgaa cgaggttcgc caccgccatg
      541 gtcgagcggc gagcgccgac cttgcggtag atccgcaaga agttgcccgt gacacgcgat
      601 tcggcgccgc cggcctttgg cttcgcgacg acgacgaacg agccgcgtta atacgcgagg
      661 tcgccggaga cgacggtctc ctcgggcgcc caagactcgt cgaagtccgt cgtcgcgaac
      721 gcgcgcgtca tggcgtcgac gagggccttc ttgcccgtca gcggcgggcc gcccgacgag
      781 atcatcatgt gcccctcggt cgtcaagctc gccagcgtct cgatgtcgcc gtcgttgatg
      841 gcttgcaagt agcgacgatt gaactcggcg agcgccggca acagtagcac gcgaccgggc
      901 gagcgaccgc agcggttcga tcgaagagca gagcgcgagc tacttcttcg cgcgatggcg
      961 caggatcgca atcacgagta gcccgatgcc gattaggaac agcgccgaag gctcaggcat
     1021 gcgcggctgc tccgatcgtg gtctttgagg ggcgaaacat agctcgtgcg gtcctggtaa
     1081 aaactgggcc aatacacgac gcatgccagc caaatagaat caatggcttg gtgattgtcc
     1141 tttgccgatt atccgagtgg ctgtaaaggg cgccgacggc cgctcggcca ctcgggtttc
     1201 taggctgaca gcgcaagcgt cgtctcgttg gctggcggaa agatcgggct cggccgtatt
     1261 aggagagtgt gaaaaccgag gctgcacgtc cgaacgaggt acccgcgtcg atggccgacg
     1321 agcccgagtc cgcggcggtc gaagatccgc gcgcggcgtt cgtgacgggc gtcttcctgc
     1381 gctttcagca ctcgctgctg cgctatctgc gcgatctctt gtcgaggcgg gaggacgccg
     1441 aggacgtcgc gcaggaaacg tatctcaagc tcgtgcatgc cggcgggctc gagcattcgg
     1501 aagtgcgcgt gcgggccttc atgttcaagg tcgcgacgaa cctcgcttac gaccgattcc
     1561 gtcagcgccg ctcgcgcggc cgacaggacg acagcgagct cgtggcgttg cccgatgacg
     1621 ccccggctgc ggaccgcgtc gtcgcattgg agcaggggct ttcgatcgtc gagcgcacgt
     1681 tgctcggctt gccggcgcgc tgtcggcagg tgttcctgct gcgggtggcg cacgagtgga
     1741 gctacgaggc gatcgccgag cgactggaag tcagcaagcg aaccgtggaa cgcgagatgc
     1801 aaacggcgct cgaagcctgc gagcggaacc acaaaaaggg tgagctgcca tgaaccgaac
     1861 ggatgcgacc gaagcgaacg acgaggcggc ggaggccgcg cgccgattcg tgcgcgccga
     1921 ttccggcagc gagccggcta acgatgccga gctcgcgagt tggctcgcgg cgcggccggc
     1981 caacgagcgt gccatgcagc gcatcgatct cgccgtcgag ctcggccgcc gcctcgcggc
     2041 cgagccgagc agcgcgctcc atgccgaagc gcaactcgct gcgcgcgctc gcccgcggcc
     2101 gtatcgaacg ccgttgctgc gcacgctcgc ctggggcggc gcgctggcgg cgctgttgct
     2161 cgtcgcgctg ttcgtcgtgc gcgacgacgt cgtgccgccg gagccgatcg tgatccaggc
     2221 ggcaaggacc gtcgcaagtg cggcgccgag cagcgccgtc gcggtgctgc cgagcggtgt
     2281 cctcgtcgat gcgagcgcgg tcgccgtgtt gccgttcgcg ggagcacgcg acgcggcgct
     2341 cgcggccggt ctggagcacg acctcgtgca agcactacgc acggtgcccg gcctctacgt
     2401 gatcgccgat gcggccgtgc agccgtacgc gcggaccgag ctcgacgctg ccgagctcgg
     2461 cggcttgctc ggcgcgcgcg ggctcgtcga cggctcggtc gagctcgccg gcggccgcgt
     2521 gcgcgtcagc gcgcgcctgc gcgaagcggc gacgggggcg acgttgtggc aggcggagct
     2581 cgatcggccc gtcgacgagt tgcgcgccgt gcgatacgag atagccgagc aagtcgccac
     2641 ggcgatgttc gattccggcg ctcgcgccgc gctcgcgccg cccgagcgcg acgacgagac
     2701 gttttccggc aagccgtttc aacagtgagg cgaacgacct gagggcgtgg cgccgcgtat
     2761 gatcgtgcga tgcgaatcgc gcatatcgag gccggcagac atttatatgg cggcgcggcc
     2821 caggtccgtt atttgatcga ggggctgacg ggccgcggcg tcgaaaattt gttgttctgc
     2881 ccgccaggca gcgaccttgg cgccgcgcta ccggcccagc aggtcgtcga gctgccgatg
     2941 cacggcgagc tcgacgtcac gttgctgccg cggctcgcgc gcactctgaa gcgcacgcag
     3001 ccggatctcg tccacgtgca ttcgcggcgc ggcgccgacc tctacggcgg ct
//
LOCUS       DATLEE010000462         3425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5737736, whole genome shotgun sequence.
ACCESSION   DATLEE010000462 DATLEE010000000
VERSION     DATLEE010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5737736"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VM692_09970"
     CDS             <1..193
                     /locus_tag="VM692_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52538.1"
                     /translation="GSEALFSIDNGRTFGRPEELTVPTANGGTRPADAADYTHVRWIL
                     SAPLDVGASGVARFRAVPR"
     gene            190..2142
                     /locus_tag="VM692_09975"
     CDS             190..2142
                     /locus_tag="VM692_09975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HUQ52539.1"
                     /translation="MNLDDVFGADGALAERLPGFTYRAAQQQMAELVVTALESGRHAV
                     IEAGTGIGKTFAYLLPVLLLERRAIISTGTHTLQDQLFARDLPLLGAVVGRPMEVAVL
                     KGRGNYLCWHRVDAALRDGTRDAATVAALEALDAWGQTSASGDLTELEDLAEDHALRA
                     QVTSTVDNCLGRECAFVDRCFVVEARRRAQAADVVIVNHHLLLADLSLKDSGFGELLP
                     GTDAVIVDEAHQLPDVAQQFFGWSVSTRELESLSRDVFAEARTAGALTRVDESLSALG
                     RAIVGLRVAVTRPDGRTPWIAAPAALRDGLPELAAALEALSGDLESLAESSAGLRNCF
                     ERCTACATRLREITVADASEGLRWFDLTAGGVAVHWTPLDVGAALAARIEAQNGVWVF
                     ASATLAVGEDFSHFLGRIGLHAPLTGVLPSPFDYERNARMYLPQGLPDPSDDSYVDAL
                     LATIWPLVEAAGGGAFLLFTSYRALQRAERWLEGRAAPGRVLVQGRGSRSDLLNQFRA
                     DGNAILLGTGSFWQGVDVRGRALRFVMIDKLPFAVPSDPLVQARAEAIRRAGGDAFNE
                     LQLPQAVLALKQGVGRLIRDFDDRGLIVLGDPRLRTRNYGAMFLASLPAMPVIDDFAA
                     ALEFATTLGHDEVAEPAAARVAGAAS"
     gene            2139..2816
                     /gene="tsaB"
                     /locus_tag="VM692_09980"
     CDS             2139..2816
                     /gene="tsaB"
                     /locus_tag="VM692_09980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HUQ52540.1"
                     /translation="MNLLGFETSSSVGSVALETPGGVVVRELATPREQTEQIIALTDE
                     LLRGAGLELDGLDGIAFGRGPGSFTGLRVSVAVAQGLAAVNGTPLLPVSSLLCLAQRV
                     SREHGWQRALVCIDAHMGEVYWAQAHERAGIVEVVGDERLGAPAEVGPLPAAPYGAVG
                     SGFAAHAEALAPLARGATEVLPAVTPSAVDLFPQAKRDLAAGRVAAASAALPVYLREH
                     TAWRRSS"
     gene            2875..>3425
                     /locus_tag="VM692_09985"
     CDS             2875..>3425
                     /locus_tag="VM692_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUQ52541.1"
                     /translation="MTQRRILVVEDERAIREMVSFNLARAGYQVISAGDAREARAAIA
                     DQRPDLVLMDWMLPDVSGLELTRQLKRDAETKEIPVIMLTARSEEDDRVAGLDGGADD
                     YIVKPFAPRELLARIRAALRRHETAEGEVVNAGTLKLDSASHQVSVDGAEVALGPTEY
                     RLLEFFMRHPDRVYSRAQLLDRV"
BASE COUNT          481 a         1146 c         1228 g          570 t
ORIGIN      
        1 cggcagtgag gcgctgttct ccatcgacaa cggccgcacg ttcggccgac ccgaggagtt
       61 gacagtgccg acggcgaatg gcggcacgcg gccggcggac gccgccgact acacgcacgt
      121 gcgctggatc ttgagcgccc cgctcgacgt gggtgcctcc ggtgtggcgc gctttcgggc
      181 ggtgccgcgt tgaatctcga cgacgtgttc ggcgccgatg gtgcgctcgc cgagcgcctg
      241 ccgggcttca cttatcgcgc ggcgcagcaa cagatggctg agctcgtcgt cacggcgctc
      301 gaatcagggc gccatgccgt catcgaggcc ggcaccggaa tcggcaagac gttcgcttac
      361 ctattgccgg tgttgctgct cgaacggcgc gcgatcattt cgacgggaac gcatacgctg
      421 caggaccaat tgttcgcccg tgacttgccg ctgctcggcg ccgtcgtcgg gcggcccatg
      481 gaggtcgcgg tgctcaaggg ccgcggcaac tacttgtgct ggcatcgcgt cgacgccgca
      541 ttacgcgacg gtacgcgcga tgcggcgacg gtcgcggcgc tcgaggcgct cgacgcgtgg
      601 ggccagacga gcgcgagcgg cgacctgacg gagctcgagg atctcgccga ggatcacgcg
      661 ctgcgcgcac aggtcacgtc gacggtagac aattgcctgg gccgcgaatg cgcattcgtc
      721 gaccgctgct tcgtcgtaga agcgcgacgc cgcgcgcaag ccgcggacgt cgtcatcgtc
      781 aatcaccatc tgttgctcgc cgatctctcg ttgaaggaca gcggcttcgg cgagctgttg
      841 ccgggcaccg acgccgtgat cgtcgacgag gcgcatcagt tgcccgacgt cgcgcagcaa
      901 ttcttcggct ggtccgtcag tacgcgcgag ctcgagtcgt tgtcacgcga cgtcttcgcg
      961 gaggcgcgga cggccggcgc gcttacgcgc gtcgacgaat cgttgagcgc cctcgggcgc
     1021 gcgatcgtcg gcttgcgcgt cgcggtgacg cgccccgatg ggcgcacgcc ttggatcgcg
     1081 gcgccggccg cgctgcgcga tgggctgccc gagctggccg ccgcgctcga ggccttgagc
     1141 ggcgacctcg agtcgctcgc ggagtcgagc gccggcttgc gcaattgctt cgagcgttgc
     1201 acggcgtgcg cgacgcggct gcgcgagatc acggtcgccg acgccagcga agggctgcgc
     1261 tggttcgact tgacggcggg cggcgtggcc gtccactgga cgccgctcga cgtcggtgcc
     1321 gcgctggccg cgcgcatcga agcgcaaaac ggcgtttggg tattcgcgtc cgcgacgctc
     1381 gccgtcggtg aggacttttc gcacttcttg ggccgaatcg gtttgcatgc gccgttgacg
     1441 ggcgttctgc cgagcccgtt cgactacgag cgcaacgcgc gcatgtatct gccgcaaggc
     1501 ttgccggacc ccagcgacga ttcgtacgtc gacgcgctgc tcgcgacgat atggccgctc
     1561 gtcgaagcgg ccggtggcgg agcattcttg ttgttcacga gctatcgcgc gttgcagcgc
     1621 gcggagcgct ggctcgaagg ccgtgcggcg cccggccgcg tgctcgtgca gggccgcggc
     1681 tcgcgcagcg acctcttgaa tcagtttcgc gccgacggca acgcgatcct gctcggcacg
     1741 ggcagcttct ggcagggcgt cgacgtgcgc ggacgcgcgc tgcgcttcgt gatgatcgac
     1801 aagttgccgt ttgccgtgcc cagcgatccg ctcgtccagg cgcgcgcgga agcgattcgc
     1861 agagccggcg gcgacgcgtt caacgagctg cagctgccgc aagccgtact agcgctgaaa
     1921 cagggcgtcg gtcggctcat ccgcgatttc gacgaccgcg ggctcatcgt gctgggcgat
     1981 ccgcgcttgc gcacgcggaa ctacggcgcg atgtttctcg cgagcttacc ggcgatgccc
     2041 gtcatcgacg acttcgccgc cgctctcgaa ttcgcgacga cgctcgggca cgacgaagtg
     2101 gccgagcccg ccgcggcccg agtcgcgggg gccgcgtcgt gaatctgctc ggtttcgaaa
     2161 cgtcgagctc cgtcggctcg gtcgcgctcg agacgccggg cggcgtcgtc gtgcgcgagc
     2221 tcgcgacgcc gcgcgagcag acggagcaga tcatcgcgtt gaccgacgag ctactgcgcg
     2281 gcgcggggct cgagctcgac gggctcgacg gcatcgcgtt cgggcgaggg cccggctcgt
     2341 ttacgggcct gcgcgtcagc gtcgccgtgg cgcaggggct cgccgccgtc aacggcacgc
     2401 cgttgctgcc ggtgtcgagc ttgctgtgcc tcgcgcagcg cgtctcgcgc gagcacggtt
     2461 ggcagcgagc gctcgtctgc atcgacgcgc acatgggcga ggtctattgg gcgcaagcgc
     2521 acgagcgcgc cggcatcgtc gaggtcgtcg gcgacgagcg gctcggcgcg cccgccgaag
     2581 tcgggccgtt gccggccgcg ccgtacggcg ccgtcggcag cggcttcgct gcccatgccg
     2641 aggcgctggc gccgctcgcg cgcggcgcca ccgaagtgct gccggctgta acgccatcgg
     2701 ccgtcgacct attcccgcag gccaagcgcg accttgcggc cggccgcgtc gccgccgcgt
     2761 ccgcggcatt acccgtttat ctgcgcgagc acacggcctg gagacgcagt tcttaacctg
     2821 cctgtaatat ttccgcggtc caatggtcgc gataaaagtg gccgcgagtc gacgatgacg
     2881 cagaggagaa tcctcgttgt agaggatgag cgcgcgatcc gcgagatggt gtcattcaat
     2941 ctcgcccgcg cggggtatca ggtgatctcg gcgggcgatg cgcgcgaggc gcgcgccgcg
     3001 atcgccgacc agcggccgga tctcgtcttg atggattgga tgttgcccga cgtcagtggc
     3061 ctcgagttga cgcgccaact gaagcgcgac gccgagacga aggagatccc cgtcatcatg
     3121 ctgactgcgc gcagcgagga ggacgaccgc gtcgcgggtc tcgacggcgg cgccgacgac
     3181 tacatcgtca agccgttcgc gccgcgcgag ctgctggcgc gtattcgcgc ggcgctgcgc
     3241 cgtcacgaaa cggccgaggg cgaggtcgtc aacgccggca cgttgaagct cgactcggcg
     3301 agccatcagg tcagtgtcga cggtgcggag gtcgcactgg gacccacgga atatcggctg
     3361 ctcgagttct tcatgcggca tccggatcgc gtctacagcc gcgcgcaatt gctcgatcgc
     3421 gtctg
//
LOCUS       DATLEE010000463         3099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9952863, whole genome shotgun sequence.
ACCESSION   DATLEE010000463 DATLEE010000000
VERSION     DATLEE010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9952863"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VM692_09990"
     CDS             complement(<1..422)
                     /locus_tag="VM692_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HUQ52542.1"
                     /translation="MQRSDFHYDLPPELIAQAPLGERRASRLLVLDGATGSLADRTFV
                     ELPQLLAPNDLLVLNDTRVLPARVLGRKPTGGRVEILVERVLGPRHALVHLRASHKPR
                     QGGDIDLPGDARARVGARLGELVEIELDRDLVPYLEGHG"
     gene            complement(473..558)
                     /locus_tag="VM692_09995"
     tRNA            complement(473..558)
                     /locus_tag="VM692_09995"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(522..524),aa:Leu,seq:caa)
     gene            1025..1417
                     /locus_tag="VM692_10000"
     CDS             1025..1417
                     /locus_tag="VM692_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUQ52543.1"
                     /translation="MLMIYNDKSLLDALPQAELDSRMRHCLAHADDLRDEGKLLDAQI
                     LAAPTAAKSVRIRNGRTTTTDGPFTETKEMIAGFNVVEADSIDDAVRMAAEFPWAKTG
                     CVEVRPIVDWADVREAVGAPQRASTARE"
     gene            complement(1400..1801)
                     /locus_tag="VM692_10005"
     CDS             complement(1400..1801)
                     /locus_tag="VM692_10005"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HUQ52544.1"
                     /translation="MTFGVAYLLARILSCGIWVAAGAHKATHYQTNIAEMGHLGVPLP
                     RLVLPLVLLLEMVGAVLMVVDRYVWLVALLWIVFMIPATWLYHVKFMMKDGTIVFPQF
                     VTGWKNVSIAGGLLALILLDASRPVWLFPGG"
     gene            complement(1834..2574)
                     /locus_tag="VM692_10010"
     CDS             complement(1834..2574)
                     /locus_tag="VM692_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006955445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HUQ52545.1"
                     /translation="MEWLTSPEAWIALTTLTLLEIVLGVDNIIFISILVGRLPEAQRQ
                     RARLLGLGFAMGTRILLLLLLTWIMRLQAALFSVGGVDISGRDLILIAGGLFLLAKST
                     LEIHESLEGAQHESHDAKRAGFVSVIIQIGLIDIVFSLDSVITAVGLADDVGVMIVAI
                     VIAVGVMMIAAGAIGRFVDDHPTVKMLALSFLVLIGVALIAEGVDVHIPKGYIYFAMA
                     FSVVVEMLNIRIRRGRGRKPLQLKKKDV"
     gene            complement(2604..>3099)
                     /locus_tag="VM692_10015"
     CDS             complement(2604..>3099)
                     /locus_tag="VM692_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HUQ52546.1"
                     /translation="NVSEETRERARVQMLEGVDPASMIVFKPASGTPTKHKITIFTDV
                     DCGYCRQFHREIDKVTALGIEVHYLFFPRTGPNTESWTKADEVWCAPDHNAALTRAKL
                     GGEIPKAPSCNTPVQAHWQLGRDVGVRGTPAIFSETGELIGGYLPPATLAKLLDDPNA
                     VVEE"
BASE COUNT          599 a          995 c          993 g          512 t
ORIGIN      
        1 ccatgccctt cgagatacgg cacgaggtcg cgatcgagct cgatctcgac gagctcaccc
       61 aagcgcgcgc ccacgcgtgc ccgagcatca ccgggcaaat cgatgtcgcc gccttgccgc
      121 ggcttatggc tcgcgcgcaa gtgcacgagc gcgtgccgtg gacccagtac gcgctcgacg
      181 agtatttcga cgcggccgcc cgtcggcttg cgtcccagca cgcgcgccgg cagcacgcgc
      241 gtgtcgttga gcacgagcag atcgttcggc gcgagcagct gcggcagctc cacgaacgtg
      301 cgatccgcga gcgagcccgt ggcgccgtcg agcacgagca gccggctcgc gcgccgctcg
      361 ccgagcggcg cttgcgcgat cagctcgggc ggcaagtcgt agtggaaatc gctgcgctgc
      421 atcggagcgc gattctagcg gcctaaagac cgaatgcgcg gtcgagtaaa agtggtgccc
      481 gaggtcggag tcgaaccgac acgctgttta ggcggcggat tttgagtccg ctgcgtctac
      541 caattccgcc actcgggctt cgagtggttc aaatttgagt cggggagtat agaggcggct
      601 aagggtgcgc gtcacgcgtc ggcactgcgc cgacgcgcac tctcgcgatg cgcgcagcag
      661 cgggggatcg cgaccaattg agctcgccgt cgatgacgcg caactaggtc aacagcgaga
      721 tggcgttcgc agaccggtgg ggcgagcccg tcacggcgat gcgccgaccc accggtagat
      781 cggcggcgtc gatgccctgt tgtagcaagc tcgcggagcc gacccattcg atcctgtata
      841 ccgtcgaatc gtccgctttg acggtgagcc agacgtgagg ccccgtccac gtggcatcct
      901 ggatttcgcc ttcgatcgac tcgctgatat agctgtggtg aactctatgg acacttcgac
      961 cgacggccgc gagaatcgag acgccccgcg aacgtaagtg ccgaggatct gccccgtgaa
     1021 gttcatgctg atgatctaca acgacaagtc gttgctggac gctctgccgc aagcggagct
     1081 cgactcgcgg atgcggcatt gcctcgcgca cgcggacgac ctgcgcgacg aaggcaagct
     1141 gctcgacgcg cagattctcg cggcgccgac ggccgcgaaa tcggtgcgca ttcgcaacgg
     1201 gcgaacgacg acgaccgacg gcccgtttac ggagacgaaa gagatgatcg cgggcttcaa
     1261 cgtcgtcgaa gccgacagca tcgacgacgc cgtgcgtatg gcggccgagt tcccgtgggc
     1321 aaagaccggc tgcgtcgagg tgcggcctat cgtcgactgg gccgacgttc gcgaagccgt
     1381 cggcgcaccg cagcgcgcat caaccgcccg ggaatagcca caccggtcgg ctcgcgtcga
     1441 gcaggatcaa cgccagcaaa ccgccggcaa tcgacacgtt tttccagccc gtcacgaact
     1501 gcggaaagac gatcgtgccg tccttcatca tgaacttgac gtgatagagc cacgtggccg
     1561 ggatcatgaa cacgatccac agcagcgcca cgagccagac gtagcggtcg acgaccatca
     1621 gcactgcgcc gaccatctcg agcaacagca cgagcggtag cacgagccgc ggcagcggaa
     1681 caccgaggtg ccccatctcg gcgatgttcg tttggtaatg cgtggcctta tgcgcgccgg
     1741 cggcgaccca gatgccgcaa ctcaagatcc gcgccaatag gtacgcgact ccaaaagtca
     1801 tgactccccc tcgcccgcgc aaacgcagcg gtcttagacg tctttctttt tcagctgcag
     1861 cggcttgcgg ccgcggccgc ggcggatacg aatgttcaac atctcgacga cgaccgaaaa
     1921 cgccatcgcg aagtagatgt accccttcgg gatgtggacg tcgacgccct cggcgatcag
     1981 cgcgacgccg atcaacacca agaagctcaa ggcgagcatc ttcacggtcg ggtgatcgtc
     2041 gacgaagcgg ccgatcgcgc cggccgcgat catcattacg ccgacagcga tgacgatcgc
     2101 gacgatcatg acgcctacgt cgtcggccag gccgacggcc gtgatgacgg aatcgagcga
     2161 gaagacgatg tcgatcaagc cgatttggat gatgacgctg acgaagcctg cacgcttggc
     2221 gtcatggctt tcgtgttgcg cgccttccaa gctttcatgg atctcgagcg tcgatttggc
     2281 aagcaagaac agaccgccgg cgatcagaat cagatcgcgg cccgagatgt cgacgccccc
     2341 cactgaaaac aacgccgctt gcagccgcat gatccacgtc agcagcaaca gcagcaggat
     2401 gcgcgtgccc atcgcgaacc cgagcccgag cagccgcgcg cgttgccgct gcgcctcggg
     2461 caggcggccg accagaatgc tgatgaagat gatgttgtcg acgccgagga cgatctcgag
     2521 cagcgtcaga gtcgtgagag caatccacgc ctcgggcgaa gtcagccact ccatcgggca
     2581 acgcccctac agcccggagg ggcttattct tcgactacgg cgttcggatc gtcgagcagc
     2641 ttggcgagcg tcgccggcgg caggtagccg ccgatcagct caccggtctc cgaaaagatc
     2701 gcaggcgtgc cgcgcacgcc gacgtcgcga ccgagctgcc agtgcgcttg gaccggagta
     2761 ttgcaagacg gagccttcgg gatttcgccg ccgagcttgg cgcgcgtcaa cgcagcgttg
     2821 tgatccggcg cgcaccaaac ctcgtcggct ttcgtccacg actcggtgtt ggggcccgtt
     2881 cgcgggaaga acaaatagtg gacctcgatc ccgagcgcgg tgaccttgtc gatctcgcga
     2941 tggaattggc ggcagtagcc gcaatcgacg tccgtgaaga tcgtgatctt gtgcttggtc
     3001 ggcgtgccgc ttgccggctt gaagacgatc atgctggccg ggtcgacgcc ttcgagcatt
     3061 tgcacgcggg cgcgctcacg cgtctcttcg ctgacgttg
//
LOCUS       DATLEE010000464         2800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4992269, whole genome shotgun sequence.
ACCESSION   DATLEE010000464 DATLEE010000000
VERSION     DATLEE010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4992269"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="VM692_10020"
     CDS             complement(<1..1763)
                     /locus_tag="VM692_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ52547.1"
                     /translation="MAQASVPLIVLSGREDDVELVNRSLRGGGHAVRCHWVTKVDALA
                     GAIDDHSPELLCFFPDSLPAPIREVAKIKQQAAGTVPLVVVSKAAQETDIAEALLAGA
                     QDLVSLGQTERLRSVAERELRSFRLERALNETLFSANQYKKQLKAFMAGSVDAIAHVQ
                     EGILVDANQAWADLLGHASAEGLLAAPLMDMFDAGSQAALKGALVACGRGQWAGGSIK
                     VVATTAGGEKLPIELNLEATSVDGDPAVKLSVPRPTVETREPEDIIEQAVHKDPMTGF
                     YHRRRFLEVLTDRLDAGSSRGVRVLAYIRPDKFREIEEQIGPLASEDVLVQLAEQLRT
                     LAQPQDLYGRFGGQVFTMFVERGTLRDVEAWAQNALARIAERVFESAHNTLSVTCTIG
                     LAEVGPSTERLDALLAEARRSSQLGRDQGGNKVVLQQTSDESTRVQRFDEIWVQQIKA
                     ALMENRFRLAHLPIASLSGEQQVMYDTVLRMIDAQGDEVGPAEFMPAAFRNRLMRAID
                     RWVIGASLAFCSKTPLDCVFVKLSYESLIDKTLLDWLAKAVQSSGVTPRTLCFQVSEE
                     NATQYLTQTQELADHLRKMGHR"
     gene            complement(1851..>2800)
                     /gene="epmB"
                     /locus_tag="VM692_10025"
     CDS             complement(1851..>2800)
                     /gene="epmB"
                     /locus_tag="VM692_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HUQ52548.1"
                     /translation="ALQAAVRDPAELLRLLDLPPDALGAPADVAFPLLVPRGFVARMR
                     KGDPHDPLLRQVWPHAAESAAVQGFSADPVREQGLAAQGVIQKYPGRVLLIASGACPL
                     HCRYCFRRDFPYQSQLAARGDWTAALAELANVPDAREAILSGGDPLSLSNRRLGELIA
                     RLADTQLATVRIHTRFPIALPERVDDGLLRVLRGTRLKTVLVVHANHANELDTSVERA
                     LSALRPALTALLNQSVLLRGVNDDAQALAGLSERLFACGVLPYYLHLLDRVAGAAHFD
                     VDETTAKKIVAALRTRLPGYLVPRLVRETPKELSKTPVA"
BASE COUNT          449 a          937 c          942 g          472 t
ORIGIN      
        1 cggtgtccca tcttgcgcaa gtggtccgct agctcctgag tctgcgtcag gtattgcgtc
       61 gcgttttcct cgctgacctg gaagcacagg gtccgtggcg tcacgcccga gctctgcacg
      121 gctttcgcga gccagtcgag caacgtcttg tcgatcaggg attcgtacga aagcttgacg
      181 aacacgcagt cgagcggcgt cttcgagcag aacgcgaggc tcgcgccgat gacccaacgg
      241 tcgatcgcgc gcatgaggcg gtttcggaag gcggccggca tgaactcggc gggcccgacc
      301 tcgtcgccct gggcgtcgat catgcgcagc acggtgtcgt acatgacctg ctgctctccg
      361 ctcaggctcg cgatcggcag atgcgcgagc cggaaccggt tctccatcaa cgcggctttg
      421 atctgctgaa cccagatctc gtcgaagcgc tgcacgcgcg tgctttcgtc cgacgtctgt
      481 tgcagcacga ccttgttgcc gccttgatcg cggccgagtt gactcgaacg gcgcgcttcg
      541 gcgagcagcg catcgagccg ctcggtgctg ggcccgacct cggcgaggcc gatcgtgcac
      601 gtcaccgaca acgtgttgtg cgcgctctcg aagactcgct ccgcgatgcg cgcgagcgcg
      661 ttctgcgccc acgcttcgac gtcgcgcagg gtgccgcgct cgacaaacat cgtgaatact
      721 tggccgccga accggccgta aagatcttgc ggctgcgcga gcgtgcgcag ttgctcggcg
      781 agctgcacga ggacgtcctc gcttgcgagc ggaccgatct gctcttcgat ctcgcgaaac
      841 ttgtcggggc gaatgtaggc gagcacgcgc acgccgcggc tgctgccggc atcgagccgg
      901 tcggtcaaga cctcgaggaa gcggcgccgg tggtagaacc ccgtcatggg atctttgtgc
      961 acggcttgct cgatgatgtc ctccggctcg cgcgtctcga cggtcgggcg cggcacgctg
     1021 agcttaacgg ccgggtcgcc gtccacggac gtggcttcga ggttgagctc gatcggcagc
     1081 ttctcgccgc cggccgtcgt ggccacgacc ttgatcgagc cgccggccca ttggccgcgg
     1141 ccgcacgcga cgagcgcgcc cttcagcgcg gcctggctgc ccgcgtcgaa catgtccatc
     1201 agcggcgcgg ccagcagtcc ttctgcgctc gcgtgcccga gcagatccgc ccacgcctga
     1261 ttcgcgtcga cgaggatgcc ctcctgcacg tgcgcgatgg cgtcgactga gcccgccatg
     1321 aacgccttca actgcttctt gtattgattc gcggagaaca acgtctcgtt cagcgcgcgc
     1381 tcgagcctga aggagcgcag ctcgcgctcg gcgacgctgc gcagccgttc cgtctggccg
     1441 agcgaaacga ggtcttgcgc gcccgcgagc agcgcctcgg caatgtcggt ctcttgcgcg
     1501 gccttgctga cgacgacgag cggcaccgtg cccgcggcct gttgcttgat cttggcaacc
     1561 tcgcggattg gcgccggcaa gctatcgggg aagaagcaca acagctccgg cgagtgatcg
     1621 tcgatcgcgc cggcgagcgc gtcgaccttc gtgacccaat ggcaacgcac ggcgtggccg
     1681 ccaccgcgaa gcgaacggtt gacgagctcg acgtcgtctt ctcgaccgct gagcacgatt
     1741 agcgggaccg aagcttgggc catgccgtag ttattctcga ggcgagagct gcagtgtaag
     1801 gacggccgct tgcgctcaga gggacgcgag tcacaatcga agcacgcgcc tcaggcgacc
     1861 ggcgttttac ttaactcctt cggcgtttcg cgaacgagcc gcggcacgag atagccgggc
     1921 agtcgcgtcc gtaacgccgc gacgattttc ttcgccgtcg tctcgtcgac atcgaagtgg
     1981 gctgcgcctg cgacgcggtc cagcaagtgc aggtagtaag gcaaaacgcc gcacgcgaac
     2041 agccgctcgc tcaagccggc cagcgcttgc gcgtcgtcgt tgacgccgcg cagcaacacc
     2101 gattgattca gcaatgccgt cagcgcgggt cgcaacgccg agagcgcgcg ctcgacgctc
     2161 gtgtcgagct cgttcgcatg gttggcgtgc acgacgagga ccgttttcag ccgcgtgccg
     2221 cgcagcacgc gcagcaagcc gtcgtcgacg cgctcgggaa gcgcgatcgg aaaacgcgtg
     2281 tggatgcgca cggtcgcgag ctgcgtgtcg gcgagccgcg ctatgagctc gccgagccgc
     2341 cggttcgaca gtgacagggg gtcgccgccg ctcaagatcg cctcgcgcgc atcgggcacg
     2401 ttcgcgagct cggcgagcgc ggccgtccaa tcgccgcgcg ccgcgagctg cgattgatac
     2461 ggaaagtcgc ggcggaaaca atagcggcag tgcagcgggc atgcgccgct cgcaatcagc
     2521 agtacgcggc ccgggtactt ttgaatgacg ccctgtgccg cgagcccctg ctcgcgcacg
     2581 gggtccgcag agaacccttg gacggctgcg ctctcggcgg cgtgcggcca gacctgccgc
     2641 agcaacggat cgtgcggatc gcctttgcgc atgcgcgcca cgaatccacg cggcacgagc
     2701 aacgggaacg cgacgtcggc cggcgcgccg agcgcatccg gcggcaggtc gagcaatctc
     2761 agaagctcgg ccgggtcgcg aaccgccgct tgtaaggcgg
//
LOCUS       DATLEE010000465         4011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_527143, whole genome shotgun sequence.
ACCESSION   DATLEE010000465 DATLEE010000000
VERSION     DATLEE010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527143"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1047)
                     /locus_tag="VM692_10030"
     CDS             complement(67..1047)
                     /locus_tag="VM692_10030"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HUQ52549.1"
                     /translation="MSNERDSEQRSERALTQLFQQAAPRPMPPAEDAEAIRRAVYAEW
                     EALTGRQHGLRRAGFAAVAAVVLAAIVWVGTGLVPSTPPAAVARVERVQGIIDTDLGM
                     RLEVGTTLVAGDSVSTRTGQVALRLASGGSLRVGPRTDLVLSSADAAELTSGSLYFDS
                     ESRRAGVPFSVTTPRGTVSDVGTQFVVRLDGERAPLDVGVRDGRVLLMTKSASGMAEA
                     GERLIAAVDTTAIRRETMVTFGADWDWADELAPPFDIDGRTVNEFLSWFSRQTGRSVV
                     FGSDAAERLARETRLSGSIDLAPLQKLSAVLTLTDLTYALEDERVVIETR"
     gene            complement(1044..1658)
                     /locus_tag="VM692_10035"
     CDS             complement(1044..1658)
                     /locus_tag="VM692_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUQ52550.1"
                     /translation="MVKRLLAHDREAFNAFFDGYFPRLYRFARARLGGDPDTTKEIVQ
                     VTLTKAIRKLASYRGEAAFFTWLCTICRHEIADHRERVGRERQHLVLTEDTPEIRAAV
                     EAIAAPECDEPEDNFRRLETTRLIQVALDQLPARYGNALEWKYVYGFSVDEIAAKLGI
                     GMEAAQSLRARAKRGFQEVYGALTSELKREALKKDGGKTTAEIP"
     gene            1824..3800
                     /locus_tag="VM692_10040"
     CDS             1824..3800
                     /locus_tag="VM692_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HUQ52551.1"
                     /translation="MTLRNHCVNSLAYLRPLLVYALVVIATLSLARVGLVLFELDRVL
                     AAKIILPVFVQGLRFDLIVVGFLLALPVALLPMFATSRRMARHGQRLLRIYVAVTFAA
                     LLFLELATPSFINQYDARPNQIFIEYLVYPKEVASTLFAAYRYQFIASLVGVALATVA
                     LWRSLPVQRPSEPMHVVGALLLTPALLVLCVLAMRSSFDHRAVNPSMVALSNDPMVND
                     LALNSTYSVLYALAEIRHEPEGGFRYGSMQESEALARVRAEMRVPATAFFSNDTPTLH
                     RQEATALRSRPKNLVIVLEESLGAEFVGALGGRPLTPNLDALAKQGWWFANLYATGTR
                     SVRGLEALITGFTPTPAPSVVKLGKSQRDFFSIARVLRDAGYDTSFIYGGEGQFDNMR
                     RFFMNNGFDFVVDEDDFSKPVFHGSWGVSDEDLFARADEEFAKARDVPFFSLVFTTTN
                     HSPYEYPAGRIDPYDADPHTVNNAVKYADHALGAFFERARSSAYWADTVFLVVADHNS
                     RVLGADLVPVEHFHIPALVLGGGIEPAVYEPVASQIDLPVTLLSLIGVSATHPMIGHD
                     LTRPEFASWPGRAIMQYDDTQAYMQGREVAILRRHLPAATFVYTEGSGLEPAPEDPEL
                     VATAIAHATWTSDTYEHLRYRPAEPERHTKLAPP"
BASE COUNT          682 a         1335 c         1237 g          757 t
ORIGIN      
        1 caggttctgc ggtccgattg tacgcaatga cccgcgcagg cgccgacgcg aggggccgcg
       61 agctcgctag cgcgtctcga ttacgacgcg ttcatcctcg agggcgtacg tcagatcggt
      121 tagcgtcaat acggccgaca gtttttgcag cggcgcaagg tcgatcgagc cgctaagtcg
      181 cgtttcgcgg gccaagcgtt cggccgcgtc gctgccgaac acgacgctgc gtcccgtttg
      241 gcgcgagaac caggaaagaa actcattgac cgtgcggccg tcgatgtcga acggcggcgc
      301 aagctcgtca gcccaatccc agtcggcgcc aaacgtcacc attgtctcgc gccgaatcgc
      361 agtcgtatcg acagcagcga tgagacgctc gccggcctcg gccatacccg acgcgctctt
      421 cgtcatcagc agcactcgac cgtcgcgcac gccgacgtcg agcggcgccc gctctccgtc
      481 gaggcgcacc acgaattggg ttccgacgtc gctgaccgtg ccgcgcggcg tcgtgacgct
      541 gaacggcaca ccggcacgcc ggctctcgga gtcgaagtac agtgagccgc tagtaagctc
      601 ggcggcgtcg gcgctcgaca gcacgaggtc cgtccgcggg ccgactcgca gcgatccgcc
      661 gctcgcaagc cgcaacgcaa cttgaccggt gcgcgtcgat accgagtcgc cggcgacaag
      721 cgtcgtgccg acttcgagtc gcatgcccaa atccgtatcg atgatgcctt gcacgcgctc
      781 gacgcgtgcg accgcagccg gcggcgtgct cggcaccaac ccggtgccaa cccagacgat
      841 ggcggccaac acgacagcag ctaccgccgc gaaacctgcg cggcgcagcc cgtgctgccg
      901 ccccgtcagg gcctcccatt cggcgtacac ggcgcgccga atggcctccg cgtcctcggc
      961 gggaggcatg ggccgaggcg cagcttgctg gaacagctgc gtcaatgcgc gttccgatcg
     1021 ctgttcgctg tcccgttcgt tgctcatggt atttccgctg tggtcttgcc gccgtccttc
     1081 ttcagagctt cacgcttgag ctcgctcgtg agcgcaccgt aaacctcttg aaagcctctt
     1141 ttcgcgcgcg cccgcaacga ctgtgcggct tccatcccaa ttccgagctt cgcggcaatc
     1201 tcgtcgacgc tgaacccgta gacgtatttc cattcgagcg cattgccgta acgggccggc
     1261 aattggtcga gcgccacctg gatcagccgc gtggtctcga ggcgccggaa attgtcctcg
     1321 ggctcgtcgc actcgggggc ggcaatcgcc tcgacggctg cgcgaatttc cggcgtgtcc
     1381 tcggtcaaca cgagatgctg acgctcgcgc ccgacacgct cccgatgatc ggcaatctcg
     1441 tggcggcaga tcgtgcataa ccacgtgaaa aaggccgcct cgccccggta cgaggcgagc
     1501 ttgcgaatcg ccttcgtcag agtcacctgt acgatctcct tggtggtgtc cgggtcgccg
     1561 ccaaggcgcg cccgtgcaaa ccggtatagc cgcgggaagt agccgtcgaa gaacgcgttg
     1621 aacgcctcgc ggtcgtgcgc gagcaggcgt tttaccaagc gtcggtcttc gagctcgtcc
     1681 gtcatctcaa aatcccaagt tcacgctcgt ttagacaggc gggggcggga aaaacggtcg
     1741 atcgctgtcc gttgcggcaa gaccgaccgg cttcgccgtg ctgcgcggtc caacagcgtg
     1801 cgttgaccac cacggtgagc accatgacct tacgtaatca ttgcgtcaat tccttggcgt
     1861 acctgcgtcc gctgctcgtc tacgccctag tcgtgatagc cacgttgagc ctcgcgcgcg
     1921 tcggcttggt gttgttcgag ctggaccgcg tgcttgccgc taaaataatt ctcccggttt
     1981 tcgtgcaggg cctgcgcttc gacctcatcg tcgtcgggtt cctactcgcc ctccccgtcg
     2041 cgttgctgcc gatgtttgcc acctcgcgcc gcatggcgcg ccacggccag cgactgctgc
     2101 ggatctacgt cgcggtcact tttgctgcgt tgctatttct cgagctggcc accccgtcct
     2161 tcatcaatca atacgacgcc aggccgaatc aaatcttcat cgagtacctc gtctatccga
     2221 aggaggtcgc gagcacattg tttgccgcct atcggtacca attcatcgcg tcgctcgtcg
     2281 gcgtggcgct cgcaacagtc gcgctgtggc ggtctctgcc cgtgcaacgg ccaagcgaac
     2341 cgatgcacgt cgtcggcgct ctgctgctga caccggcgtt gctggttttg tgcgttctcg
     2401 ccatgaggtc gagcttcgac caccgcgcgg tgaacccgag catggtggcg ctctcgaacg
     2461 accccatggt caacgacctg gcgctgaatt ccacttactc cgtgctctac gcgctcgccg
     2521 agattcgcca tgagccggaa ggtgggtttc gctacggctc gatgcaggag tccgaggcgc
     2581 tcgcccgtgt gcgcgcagag atgcgggttc cggcgacggc gttcttcagc aacgacactc
     2641 cgacgctgca ccgtcaagag gcgacggcac tgcgttcgcg gccgaaaaat ctcgtcatcg
     2701 tgctcgagga gagcttgggg gcagagttcg taggagcact cggcggcagg ccgttgacgc
     2761 cgaacctaga cgctttggct aagcaaggat ggtggttcgc gaatctgtat gccacgggca
     2821 cgcggtcggt gcgcggtctc gaggcgctga tcacgggctt tacgcccacg ccggcaccga
     2881 gcgtagtgaa actcggcaag tcgcagcgag acttttttag catcgcccgc gtcttgcgcg
     2941 atgcggggta cgacacgagc ttcatctacg gcggcgaggg gcaattcgac aacatgcgcc
     3001 ggttcttcat gaacaacggc ttcgacttcg tcgtcgatga ggacgacttc tcgaagcccg
     3061 tgttccacgg atcgtggggc gtctccgacg aggatttatt cgcacgcgcc gacgaggaat
     3121 ttgcgaaagc gcgcgacgtc ccgttctttt ctttggtctt cacgacgacg aaccattcgc
     3181 cgtacgagta tccggcggga cgcatcgatc catacgacgc cgatccgcac accgtcaaca
     3241 acgccgtcaa gtacgccgac catgcgctcg gcgctttttt cgagcgggcg cgctcgagcg
     3301 cttactgggc ggacactgtt ttccttgtcg ttgccgacca caactcgcgc gtgctcggcg
     3361 cggacttggt gccggtcgaa cattttcaca tccccgccct cgtcctaggt ggagggatcg
     3421 agcccgccgt ctatgagccc gtggcaagtc agatcgacct ccccgtgacg ttgttgtcgc
     3481 tgatcggcgt gtctgcgacg cacccgatga tcggtcacga tctgacacga ccggagtttg
     3541 ccagctggcc aggccgagcc atcatgcagt acgacgacac gcaggcttac atgcagggac
     3601 gcgaagtcgc aattcttcgc aggcatctgc ccgccgcgac tttcgtctac accgagggtt
     3661 cggggctcga gccggcgccg gaggatcccg agctggtggc gacggccatc gcgcacgcga
     3721 cctggacgtc cgacacgtac gagcacttac gctatcggcc agctgagccg gagcgccaca
     3781 ctaagttggc gccaccgtag atccgtcgcg aacgcgatgc ttgcgggccg ggttgctcac
     3841 gcggtcaggc cgtggcaaac gctgaacccg acgaggaagc ccacgacgtt cgccgccagg
     3901 ggcgcaacgc ccagcggcac gagctcggcg accacgacca agttgaggca cgccgcggcc
     3961 gtgccgacca gcgtgaacaa ggcgaactcg cgagctcgga tcatcgttcg g
//
LOCUS       DATLEE010000466         6003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5740003, whole genome shotgun sequence.
ACCESSION   DATLEE010000466 DATLEE010000000
VERSION     DATLEE010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5740003"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1100)
                     /locus_tag="VM692_10045"
     CDS             complement(42..1100)
                     /locus_tag="VM692_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HUQ52552.1"
                     /translation="MAVVRYEFDSFSLDPLERRLFAGEALVELNSRYFDALLLLLQHP
                     GTLLSKERFLQEVWKGIPVTDEVLTQCIKTLRKQLRDSATHPRLIETVPKHGYRFIAA
                     VSHVAAGDAARAETLAPRVATTSAYDWRQCWLTLGAGTTGGGVAGLLGGLAFGFGAAS
                     QTQQSGIGALSTLLVLVALATLLGLIGAAGVAGGIAAARLVARNFAFPALVGGAVGGL
                     LVGAFGEMIGHDAFLLLFGHAPGDITGAVEGLLLGAAVGLADDLGRRFAAGSSTQRKL
                     LLAAAIGAVAGVLIAVVGGQLLGGSLAQLADSFPDARLHLDPLGALLGPFAAGATNAL
                     EGAWFSLCLVGALILARR"
     gene            1184..1657
                     /locus_tag="VM692_10050"
     CDS             1184..1657
                     /locus_tag="VM692_10050"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HUQ52553.1"
                     /translation="MTTERDAPKRWSNWTIEIKVWKILAALIVLPILVLWFRVVHQDD
                     EILALARSDVLTNAGQRTWAGSFVNTNERPLRDVAVTVDFLDDQNRPVGKAEAEADEL
                     AFGMRLDLQAPLPRDAVRLRIDSVQWRMDRRGVLMGPFREPWEFGYLMVEPVKIE"
     gene            1654..2568
                     /locus_tag="VM692_10055"
     CDS             1654..2568
                     /locus_tag="VM692_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52554.1"
                     /translation="MSAPSVPGSDVDALFKLPLDEFTSARNALAAQLKRDGKQTEASE
                     AKALVKPSVSAWVVNQLYWRHRESFDRLIEAGDRLRHVQGAQLTSDSARDAMNARREA
                     VTALAARAADLLSDGQSAATRDLLRRVTSTLEALSSYGSLPGAPAAGRLTDDVEPPGF
                     EALAGLVPSRAVARTQPSRASSSTKPAPRADARETVRRRKDAERKQQLAAAKATVRQA
                     EGALRAARKQAERAAANLDSAATRANDVEQQRAELEVQLARAAKEAKAARERADEAAA
                     NASEAAAAAEGAERALEGALRRLQQLSD"
     gene            complement(2592..3605)
                     /locus_tag="VM692_10060"
     CDS             complement(2592..3605)
                     /locus_tag="VM692_10060"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52555.1"
                     /translation="MRRLPTALYALAATLPFAAPAFAHHSQSMFDTTKEILVEGKVAR
                     FDWVNPHMYLVVETTGPDGKPALVEGEGVGITQALVDGLDRDALKPGTPVVMRANPNR
                     GGWGKQVRIMDVTTQDGEVHPFYAANTRTRVLTPAKTLEGHWAPSRAALGAGFGAMAR
                     WPVTPEGRAAQAKLLADGLCYVEPVPFLSVLDELRTIELGDKEVVMRFDNTGDHVERI
                     IHMTATHPADLKPSLHGHAIGRWEGETLVIDTIGFEPNPSGLGMNVPSSPGKHVVERL
                     TLTPDKVRIRYEVTVEDPTYLTGPGTITQQWDHRPDLEFSQDTGGCDPEVASRYKASV
                     PQQ"
     gene            complement(3747..3929)
                     /locus_tag="VM692_10065"
     CDS             complement(3747..3929)
                     /locus_tag="VM692_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52556.1"
                     /translation="MTPDLDGVPLRNLIRASGVSFFTIRVLQRWCRGEITTDAAVELI
                     QSDEAERIIGTWTNRT"
     gene            complement(4044..4181)
                     /locus_tag="VM692_10070"
     CDS             complement(4044..4181)
                     /locus_tag="VM692_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52557.1"
                     /translation="MTAIGAIPLRGRERKRLGHLTHSQVERLLIAAELRKKAIEGERD
                     E"
     gene            4302..4517
                     /locus_tag="VM692_10075"
     CDS             4302..4517
                     /locus_tag="VM692_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52558.1"
                     /translation="MSAQSRTTDTARKLRRAPPGFVLLKTAERKLREGNVCDAVRLFR
                     AIVHRYPPSLERLAAVSYLRAAHPMFV"
     gene            4689..>6003
                     /locus_tag="VM692_10080"
     CDS             4689..>6003
                     /locus_tag="VM692_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ52559.1"
                     /translation="MSAGPAGAQDCPGERDLVLTNGTILTLDAADRTVSTVRIRANRI
                     LTVGETVDRRERCVDVVDLGGRTVIPGLIDNHTHFVRTAQAPGPFVAGLESAASIREL
                     QDAFAAAATKAEPGEWITAIGGFTPRQFAERRLPTREELTAAVAEHPVYLQVGYSTRG
                     IVNDAGRRALASAGISTSEQGDVAPDGNGLTFVLRSASEERMKRRFPEYMRYAVSLGL
                     TTVVDHACCDWLGAHLTAADRPNLKIAEQFWRAGQWPLRLRIQYDHRDTRDQSNVRSA
                     TARIENATYGLGDDMFKAVRFGEQVIAGGATDSEVLDVYQRIAAAGWPLSQHTIREAE
                     IERYLRIMEQVAEKTPLTPLRWSLEHVFEITPQQIARLKAIGVGVRVQDHDYIRNDFS
                     NWNAGPPFRTLLESGIMMGAGTDAGVVGPLNPWLAIYFMVTGKDAS"
BASE COUNT         1030 a         2008 c         2032 g          933 t
ORIGIN      
        1 gccccaggcg cgggcaagct ctgcagtaga cgctcttgag ctcaacgccg cgcgagaatc
       61 agcgcgccca cgagacacag actgaaccac gctccttcga gcgcgttcgt cgcgccggcc
      121 gcaaacggcc cgagcaaggc accgagcggg tcaagatgca ggcgcgcgtc gggaaaactg
      181 tcggccagct gcgcgaggct gccgccgagc agctgccctc ctaccacggc gatcagcacc
      241 ccggccactg cgccaatggc ggcggcgagc agcaattttc gctgcgtgct cgagcctgcg
      301 gcgaagcgtc ggccgagatc gtccgcgagg cctaccgccg cgccgagcaa caaaccttcg
      361 actgcgcccg tgatatcgcc gggcgcgtgg ccgaacagca gcaggaacgc gtcgtggccg
      421 atcatctcgc cgaacgcgcc cacgagcaga ccgccgacgg cgccgccgac gagcgccgga
      481 aaagcgaagt tgcgggcaac gagtcgcgcg gcggcgatac cgcccgcgac gccggccgcg
      541 ccgatgaggc cgagcagtgt tgccaatgcg acgagcacca gcagcgtcga caaggccccg
      601 atgccgctct gctgcgtctg cgaggcggcg ccgaagccga atgcgagacc gccgagcaga
      661 ccggctacgc cgccgccggt cgtgccggcg ccgagcgtca gccagcactg acgccagtcg
      721 taagcgctcg tagtcgctac tcgtggcgca agcgtttcgg cacgcgcagc gtcgccggcc
      781 gctacatggc tcacggctgc gatgaagcga taaccgtgct tgggaacggt ttcgatcaga
      841 cgcgggtgcg tggcgctatc gcggagttgc ttgcgcaagg ttttgatgca ctgcgtcagg
      901 acttcgtctg tgacggggat gcccttccaa acttcctgca agaaccgctc cttgctgagc
      961 agcgtgccgg ggtgttgcag cagcaacagc agtgcgtcga agtaacggct gttgagctcc
     1021 acgagcgctt ctccggcaaa cagccggcgc tcgagcgggt cgaggctgaa actgtcaaat
     1081 tcgtagcgaa caacagccat tcagcccttc ctcagggttt gctcatgtga ttcagcgcgg
     1141 cgggcgccag cctgagcact cccatcgcaa ccgaggaacg agcatgacga ccgaacgaga
     1201 cgcgcccaaa cgatggagca actggaccat cgaaatcaag gtatggaaga tcctcgctgc
     1261 attgatcgtc ctgccgatcc tggtgctgtg gtttagggtc gtgcaccagg acgacgagat
     1321 tctggctctg gcccgcagcg acgtgctgac gaacgcgggc cagcgcacct gggccggcag
     1381 cttcgtcaac accaatgaac gacccttgcg cgacgtggcc gtgaccgtgg actttctcga
     1441 cgaccaaaac cgccccgtgg gaaaggccga agcggaggcc gacgagctcg cgttcggcat
     1501 gcgactggac ctgcaggcgc cgctgccgcg cgacgccgtc cggctgcgca tcgattcggt
     1561 acagtggcgc atggaccgca gaggtgtgct gatgggtccg ttccgcgaac cctgggagtt
     1621 cggttacctg atggtcgagc ccgtgaagat agaatgagcg cgccctcagt ccccggcagc
     1681 gacgtcgacg cgctgttcaa gttgccgctc gacgagttca cgtccgcgcg caacgcgctg
     1741 gccgcgcagc tgaagagaga cggcaagcaa accgaggcga gcgaggccaa ggcgctcgtg
     1801 aagccgtcgg tctcggcttg ggtcgtcaat cagctctatt ggcgtcatcg cgagtcgttc
     1861 gatcggctca tcgaggccgg cgaccggctg cgtcatgtcc aaggcgcgca actgacgagc
     1921 gactcggcgc gggacgccat gaatgcgcga cgcgaagcgg tgacggcgct cgctgcgcgc
     1981 gctgcggact tactcagcga cggtcaatcc gccgcgaccc gcgatctgct gcgtcgcgtc
     2041 acgagcaccc tcgaggcgtt gtcgagctac gggtcgctgc cgggcgcccc ggccgccggg
     2101 cggctcaccg acgacgtcga gccaccgggg ttcgaggcgc tggcggggct cgtgccgagc
     2161 agggcggttg ctcgcacgca gccgtcgcgc gcctcgtctt cgaccaaacc tgcgccgcgc
     2221 gccgacgcgc gcgaaaccgt gcggcggcgc aaagacgccg agcgaaagca acagctggcc
     2281 gccgcgaagg ctaccgtgcg acaggctgaa ggcgcgctgc gagctgcgcg caagcaagcg
     2341 gagcgcgccg cggccaatct cgactccgcc gcgacgcgcg cgaacgacgt cgagcagcag
     2401 cgtgccgagc tcgaagtgca gctggctcga gccgccaaag aggcgaaagc agcacgcgag
     2461 cgcgccgatg aagccgccgc caacgccagc gaggcggcgg cggcagccga gggcgcggag
     2521 cgcgccctcg agggtgcact ccgccggtta cagcagttgt cggattgagc gcaaccgctc
     2581 gagccgcagc cttactgctg cggcacgctc gccttgtacc ggctcgcgac ctcgggatcg
     2641 cagccaccgg tgtcctgcga gaactcgagg tcggggcggt gatcccactg ctgcgtgatc
     2701 gtgcccgggc ccgtcagata cgtcgggtct tcgaccgtga cctcataacg gatgcgtacc
     2761 ttgtccggag tcagcgtcag ccgctcgacg acatgcttgc ccgggctcga cggcacgttc
     2821 atgccgagcc cggacgggtt cggctcgaaa ccgatcgtat cgatcacgag cgtctcgcct
     2881 tcccagcggc cgatcgcgtg cccgtgcagc gagggcttca agtcggcggg atgcgttgca
     2941 gtcatgtgga tgatgcgctc gacgtgatcg cccgtgttat cgaaccgcat cacgacctct
     3001 ttgtcgccga gctcgatcgt gcgcagctcg tcgagcacgg agaggaacgg aacgggctcg
     3061 acgtagcaaa gcccatcggc gaggagcttc gcctgcgctg cacggccttc cggcgtgacg
     3121 ggccagcgcg ccatggcgcc gaagcccgcg ccgagcgcag cgcgtgaggg cgcccagtga
     3181 ccctcgaggg tcttggccgg cgtcagcaca cgcgtccgcg tgttggccgc gtagaacgga
     3241 tggacctcgc cgtcctgagt cgtgacgtcc atgatgcgga cctgtttgcc ccacccgccg
     3301 cgattcggat tggctcgcat gacgacggga gtgcccggct tcagcgcgtc gcgatcgagg
     3361 ccgtcgacga gcgcctgcgt gatgccgacg ccttcacctt cgaccagcgc cggcttgccg
     3421 tccgggcccg tagtctcgac gacgaggtac atgtgcgggt tcacccaatc gaaccgcgcg
     3481 accttgcctt cgaccagaat ctccttcgtc gtgtcgaaca tcgactggct atggtgcgcg
     3541 aaagccggcg ctgcgaaagg cagcgtcgcg gcgagggcgt agagggccgt gggcagacgt
     3601 cgcatggcga gacctcgggt caacaacata gcggaatgat aatgagatca acggcgacga
     3661 accacttggg ccgacgagcg tctggtgtgg cgcaattcgc cgcgtcgagc cgaacgcagc
     3721 ggcctacgag tgagcggcgg ccgacgctaa gtgcggttgg tccaagtccc gataatgcgc
     3781 tcggcctcgt cggactgaat cagctcgacg gccgcatcgg tcgtgatctc gccacggcac
     3841 cagcgctgca gtactcgaat cgtgaagaag ctgacacccg atgccctgat caggttgcgg
     3901 agggggacgc cgtcgaggtc cggtgtcatg tcgatacgga tgcacgacgc gtgccagtga
     3961 aagcgccggc cacacgccct atagggcact cggacgtcgg caaccgtgga ttgccgaagg
     4021 ttgcaagcgc gaaaggccgg aggttattcg tcgcgttcgc cttcgatcgc ttttttgcgc
     4081 agctcggctg cgatgagcag gcgttcgact tgtgagtgcg tcagatggcc gaggcgcttc
     4141 cgctctcgtc ctcgcaacgg aatcgcgccg atggccgtca cgctttgtcg ccgccgtccg
     4201 ctagcgcaga tgttattggt ttgtcaccgt gatgagactg ctctggcacc tcgcgctggt
     4261 tagcatgaaa ttgcaggtca tggccagcga cggtggacga gatgtcagcg cagtcacgga
     4321 cgacggatac ggctaggaag ctgcggcgcg cgccgccggg tttcgtgttg ctgaagaccg
     4381 ctgaacgcaa gctccgcgag ggcaacgtct gcgacgccgt gaggttgttc cgcgcgatcg
     4441 tgcatcgcta cccgccgtcg ctcgagagat tggccgccgt gtcgtatctg cgagccgcgc
     4501 acccgatgtt cgtgtaaccg ccgatcgcga ttccgcctcg agacgctcgg gcggtctgca
     4561 cggtctaccg ccccgacccc cgcttggctt aacctcgtcg cgtgccgaca cgagcgaaga
     4621 cccttttacc gcgccgagtc gcaggctccg cctccctcgt cgcggggctc gcggcgctcg
     4681 gattgctctt gtccgcaggc cccgccggcg cgcaggactg tcccggcgag cgcgatctcg
     4741 tcctcacgaa cggcacgatc ctcacgctcg atgccgcgga caggaccgtg agcacggtac
     4801 gcatccgcgc gaatcgcatc ctgacggtcg gcgaaactgt cgatcggcgc gagcggtgcg
     4861 tcgacgtcgt cgacctcggc ggccgcaccg tgatcccggg actcatcgac aaccacacgc
     4921 acttcgtgcg caccgcgcag gcgccgggcc cgttcgtcgc ggggctcgag tcggctgcat
     4981 cgattcgcga gctgcaagac gcgtttgctg ccgctgcgac gaaagccgag cccggcgagt
     5041 ggatcacggc gatcggcggg ttcacgccgc gccagttcgc cgagcgccgg ctgccgacgc
     5101 gcgaggagct gaccgcggcc gttgccgagc accccgtcta cctgcaggtc ggctactcga
     5161 cgcgcggcat cgtcaacgac gcgggccggc gcgcgcttgc atcagcgggc atctcgacgt
     5221 ccgagcaggg tgacgtcgcg cccgacggca acggcctcac gttcgtgctg cgctcggcga
     5281 gcgaagagcg catgaagcgg cgcttccccg aatacatgcg ctacgccgtc tcgctcgggc
     5341 tcacgacggt cgtcgaccat gcgtgctgcg attggctcgg cgcacatttg acggcggccg
     5401 atcggccgaa tctcaagatc gccgagcagt tctggcgcgc cgggcagtgg ccgctgcggc
     5461 tgcgcatcca atacgaccat cgcgacacgc gcgaccagag caatgtgcgg tccgccacgg
     5521 cgcgcatcga gaacgcgact tacgggctcg gcgacgacat gttcaaagcc gtgcgcttcg
     5581 gcgagcaggt catcgccggc ggcgcgacgg actcggaggt actcgacgtc taccagcgca
     5641 tcgcagcggc gggctggccc ctgtcgcagc acacgattcg cgaagccgag atcgagcgtt
     5701 atctgcgcat catggagcag gtggcggaga agacgccgct cacgccgctg cgctggtcgc
     5761 tcgagcacgt gttcgagatc acgccgcagc agatcgcgcg tctcaaagcc atcggcgtcg
     5821 gcgtgcgcgt gcaggatcac gactacatcc gcaacgactt ctcgaactgg aacgccggcc
     5881 cgccgtttcg cacgctactc gagagcggca tcatgatggg ggccggcacc gacgccggcg
     5941 tcgtgggtcc gctgaatccg tggctcgcga tctacttcat ggtgaccggc aaggacgcga
     6001 gcg
//
LOCUS       DATLEE010000467         4694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9463185, whole genome shotgun sequence.
ACCESSION   DATLEE010000467 DATLEE010000000
VERSION     DATLEE010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9463185"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1633
                     /locus_tag="VM692_10085"
     CDS             152..1633
                     /locus_tag="VM692_10085"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HUQ52560.1"
                     /translation="MHPRTGLWVLPALAALAACGRAPEPAAPQAARDVAQPAGWADDL
                     AMPIPQDLNPDPNVLEIDLEARISEVEIIPGFKTPAWTYNGAVPGPMIRAKVGDRLIV
                     HFTNSLPEPTTIHWHGLRVPNDMDGAPGATQEPVPTGGAFRYEFELRDAGTYWYHPHT
                     DSSAQVGRGLYGAILVEDPADPKAFGDDLVLLLSDMGLNEQGELLPADSGGNFGDLFG
                     REGAVLLVNGKVLPTLKVRNGKQQRWRIINATRARYYNIRLRNHRFMRLGGDNGLAAR
                     AEDIYNLIVTPGERADAVFTPADAPSTTNVLRWVPTERGYGSTFNRASEQMLKIETVA
                     DAPVTPEPIPTQLRTIEPLDVTNAQQRTLNLTIAITSTVEMGINGVPHWNAKPLDIEL
                     GATEVWRIVNDTDFSHPFHLHGYFFQVLDDTRVPEWKDTVNVPTKSELTVAVRFDDRP
                     GAWMFHCHILDHADAGMMGHLIVRDPKAPPADAPPAPPAPHHH"
     gene            complement(1627..2043)
                     /locus_tag="VM692_10090"
     CDS             complement(1627..2043)
                     /locus_tag="VM692_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ52561.1"
                     /translation="MPLLADHIDHIVLTVADIEATTRFYEQALGFTRETFRGPDGQQR
                     FALTFGRQKINLQDKSTVTPTKAQRPTLGSGDFCLIAAAPLDEVLAHLRRLEIAIEAG
                     PVPRRGAVGALRSIYFRDPDGNLVEVAEYVEARAPQ"
     gene            complement(2055..2378)
                     /locus_tag="VM692_10095"
     CDS             complement(2055..2378)
                     /locus_tag="VM692_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52562.1"
                     /translation="MSYEILLEEREPIDGWTVCTVCLSVDRTASVLRRRWRCADCGVD
                     TSNNSQRPLREYLTENGAAVIEKNLQEWSNVKGVRAAYKMLRSARYKCLLVLERRLRD
                     APPVG"
     gene            complement(2385..2864)
                     /locus_tag="VM692_10100"
     CDS             complement(2385..2864)
                     /locus_tag="VM692_10100"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HUQ52563.1"
                     /translation="MVRSIMLAVAACTLGASLATAEPKKDIGYDARADAFAQLDSAVA
                     AAKAEDKLVLLISGGDWCIWCHYLADFLERETAVDEALHDVFVVQKVYVGDENLNKEF
                     FARLPKAAGAPHFWVLSATGEVLKSQDTLPFEDGKKSYVPSKLMAFVDEWRKRPAAH"
     gene            3011..4168
                     /locus_tag="VM692_10105"
     CDS             3011..4168
                     /locus_tag="VM692_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HUQ52564.1"
                     /translation="MKLSTDRILTTHVGSLPRGAAVVDWLYKKDNGEPYDRAAFDAAV
                     TAGVADAVAKQLAAGVDVVSDGETSKVGYATYIKDRLSGFAGHHTRPPHLDLAPYPEL
                     RQALGRMLGPQSFKRAACVAPIELVDRAAMQQDLANLSAALATHGGAGEAFMNAASPG
                     VISSFQSNRYYATHEAYVDAIAAAMKHEYDAIVAAGFVLQLDCPDLAMARHTGFQELS
                     ESEFLARAAYQVEALNHAVRDIPADRMRMHVCWGTYEGPHDHDIALEKILDLVLRAKP
                     AGILFEAANPRHRHEWAVWREARLPEDKVLIPGCISSTSNYVEHPRLIAEQLCQYAQI
                     VGRERVIAGADCGFGSFAGYSRVDPAICYKKLRALADGAALATDRLWRSQR"
     gene            4150..>4694
                     /locus_tag="VM692_10110"
     CDS             4150..>4694
                     /locus_tag="VM692_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52565.1"
                     /translation="MAKPTLDTPLPAGPEPTAAEVRAALERILRSTCFEHAGRASDFL
                     RFVVNQTLAGDGERLKGYTIAVEVFGRPPDFDAQSDPLVRVEALRLRQRLTEYYAGEG
                     AADPVKLDLPRGGYVVKAAYASSEPASAAEPSGSSAEQPRAHRSWLAANGRVASAAAA
                     LLLIVTAAVVALQWQTPVAELR"
BASE COUNT          805 a         1620 c         1515 g          754 t
ORIGIN      
        1 tgccggaaca acccctcgga gcccggagtc tacggcattg ccagcggccg ccgcggcggc
       61 agcgtcagta attgtaccga tggcgtccac cgccgggtcc cggcaaactg gccatattcg
      121 aagttttctc cacaggaata acgagttgca cttgcacccg cgaaccgggc tgtgggtttt
      181 gccggcgctg gccgctctgg cagcgtgcgg gcgcgcgccc gagcctgcgg cgccgcaagc
      241 ggctcgcgac gtggctcagc cggccggctg ggccgacgac ctcgcaatgc cgattccaca
      301 ggacttgaac cctgatccaa acgtgctcga gatcgacctc gaagcgcgca tcagcgaagt
      361 ggaaatcatc cccggcttca agacgccggc atggacctac aacggcgcgg tgccgggccc
      421 gatgattcgc gccaaggtcg gggaccgcct catcgtccac ttcacgaatt cgctacccga
      481 gccgacgacg atccactggc acggactgcg tgttccgaac gacatggacg gcgcgcccgg
      541 ggccacgcag gaaccggttc cgaccggcgg cgcgttccgc tacgagttcg agctgcgcga
      601 cgccggcacg tattggtacc acccgcacac ggactcgtcg gcgcaagtcg gccgcggtct
      661 gtacggcgcg atcctcgtcg aggacccggc cgatccgaag gcgttcggcg acgacctcgt
      721 cttgctgctg tccgacatgg ggctcaacga gcaaggcgag ctgctgccgg ccgacagcgg
      781 cggcaacttc ggcgacttgt tcggccgcga gggcgccgtg ctgctcgtca acggcaaggt
      841 gctgccgacg ctgaaggtgc gcaacggcaa gcagcaacgc tggcgcatca tcaacgcgac
      901 gcgcgcgcgc tactacaaca ttcgtctgcg caaccaccgc ttcatgcgac tcggcggcga
      961 caacggcctc gcggcccgtg ccgaggatat ctacaacttg atcgtgacgc ccggcgagcg
     1021 cgccgacgcc gtgttcacgc cggccgacgc gccgagcacg acgaacgtcc tacgttgggt
     1081 cccgaccgag cgcggctacg gcagcacgtt caaccgcgct tcggagcaga tgctgaagat
     1141 cgagaccgtc gccgacgcgc ccgtgacgcc ggagccgatc cccacgcagc tacggacgat
     1201 cgagccgctc gacgtcacga acgcgcagca gcgcacgctg aatctcacga tcgcgatcac
     1261 gtcgacggtc gaaatgggga tcaacggcgt gccgcattgg aacgcgaagc cgctcgacat
     1321 cgagctcggc gccaccgaag tctggcgcat cgtcaacgac acggattttt cgcatccttt
     1381 ccacttgcac ggttatttct tccaggtgct cgacgacacg cgtgttcccg agtggaagga
     1441 cacggtcaac gtgccgacga aatcggagct gacggttgcg gtgcggttcg acgataggcc
     1501 cggcgcctgg atgtttcact gtcacatcct cgatcacgcc gatgccggca tgatgggcca
     1561 cctcatcgtg cgcgacccga aggcgccgcc ggccgacgca ccgccggcac cgccggcacc
     1621 gcaccatcac tgaggcgcgc gagcttcgac gtattcggcg acctcgacga ggttgccgtc
     1681 gggatcgcga aaataaatag agcgcaacgc gccgacggcg cctcgccgcg gcacgggtcc
     1741 ggcctcgatc gcaatctcga gcctgcgcag gtgcgcgagc acctcgtcga gcggcgcggc
     1801 cgcgatcaag cagaaatcgc cggagcccaa cgtcggccgc tgcgccttcg tcggagtgac
     1861 ggtgctcttg tcctgcaggt tgatcttctg ccgtccgaac gtgagcgcga accgctgctg
     1921 gccgtcgggc ccgcggaacg tttcgcgcgt gaagccgagc gcttgctcgt agaaccgcgt
     1981 cgtggcctcg atgtcggcga cggtcaggac gatgtgatcg atatggtcgg cgagtagcgg
     2041 cacgaacgca ctcttcaacc gaccggcggc gcgtcgcgca accgccgctc gagcacgagc
     2101 aagcacttgt aacgcgcgga gcgcagcatc ttgtacgcgg cgcgcacgcc tttcacgttg
     2161 ctccactcct gcaggttctt ctcgatcacc gcagcgccgt tctcggtcaa gtattcgcgt
     2221 agcgggcgct gactgttgtt cgacgtgtcg acgccgcagt ccgcgcagcg ccagcgccgc
     2281 cgcaagaccg atgccgtgcg gtcgacgctc aagcagaccg tgcagacggt ccagccgtcg
     2341 atcggctccc gttcctccag gagaatttcg tagctcatgc gtgactagtg cgcggccggg
     2401 cgcttgcgcc actcgtcgac gaatgccatg agcttgctcg gaacgtaact ttttttgccg
     2461 tcctcgaacg gcaacgtgtc ctgcgacttc agcacttcgc cggtcgcgga cagaacccag
     2521 aagtgcggag ctccggccgc cttcggcagg cgcgcgaaga actccttgtt caggttctcg
     2581 tcgccgacgt agaccttctg cacgacgaac acgtcgtgca acgcctcgtc aacggccgtc
     2641 tcgcgctcca ggaagtcggc caaataatgg caccaaatgc accagtcacc gccggagatc
     2701 agcagcacga gcttgtcctc ggccttggcc gctgcgacgg ccgagtcgag ctgcgcgaaa
     2761 gcgtcggccc gcgcgtcgta gccgatgtcc ttcttcggct ccgccgttgc cagtgacgcg
     2821 ccgagcgtgc acgccgcaac tgcgagcatg atcgatctca ccatggattt tctccgccct
     2881 cgacccaata gaccatggcg ttcgcgccgc agtcaatcag cgacgcggcc cgcatggagc
     2941 caggattttt gtggtgccgt aagacacaat gtgcgccgcc tcgaaccgcc tcgaggccac
     3001 gacgctcccg atgaagctca gcaccgatcg aatcctcacg actcacgtcg gcagcctccc
     3061 gcgcggtgct gccgtcgtcg attggctcta caagaaggat aacggcgagc cgtacgatcg
     3121 cgccgccttc gacgcggccg tgacggccgg cgtcgccgac gccgtcgcga agcagcttgc
     3181 cgccggtgtc gacgtcgtca gcgacggcga gacgagcaag gtcggttacg cgacgtacat
     3241 caaggacagg ttgtccgggt tcgccgggca ccatacgcgc cccccgcatc tcgatctcgc
     3301 gccgtacccc gagctccgcc aagcgctcgg gcgcatgctg gggccgcagt cgttcaagcg
     3361 cgcggcctgc gtcgcgccaa tcgagctcgt cgatcgcgcg gcgatgcagc aagacctcgc
     3421 gaatctcagt gcggcgctcg ccacgcacgg cggcgccggc gaggcgttca tgaacgcggc
     3481 ttcgccgggc gtgatctcgt cgtttcaatc gaatcgctac tacgcgactc acgaggccta
     3541 cgtcgatgcg atcgccgcgg cgatgaagca cgaatacgac gcgatcgtcg cggcggggtt
     3601 cgtgctgcag ctcgattgcc ccgatctcgc gatggcgcgg cacacgggct ttcaggagct
     3661 cagtgaaagc gagttccttg cgcgcgccgc ctatcaagtc gaggcactga accacgccgt
     3721 gcgcgacatt ccggccgacc gcatgcgcat gcacgtctgc tggggcactt acgaaggccc
     3781 gcacgatcac gacatcgcgc tcgagaagat tctcgacctc gtgttgcgag cgaagccggc
     3841 cggcattctg ttcgaggcgg cgaatccgcg gcaccgacac gaatgggccg tatggcgcga
     3901 agcgcggctg cccgaggaca aggtcttgat cccgggctgc atcagctcga cgtcgaatta
     3961 tgtcgagcat ccgcggctga tcgccgagca gctctgccaa tacgcgcaga tcgtcggccg
     4021 cgagcgcgtc atcgcgggcg ccgattgtgg tttcgggtcg ttcgcaggct atagtcgcgt
     4081 ggatcccgca atctgttaca agaagctaag agcgctcgcc gacggcgcag cgctcgcgac
     4141 ggacaggcta tggcgaagcc aacgctagac accccgctgc cggccggccc cgagccgacg
     4201 gccgccgaag tgcgcgccgc actcgagcgc attttgcgca gcacgtgctt cgagcacgcg
     4261 gggcgagcgt cggacttcct gcgcttcgtc gtcaaccaga ctttggccgg cgacggcgag
     4321 cgcctcaagg gctacacgat cgcggtcgag gtgttcggcc ggccgccgga tttcgacgcg
     4381 cagtccgacc cgctcgtgcg tgtcgaggcg ctgcggctgc gccaacggct cacggagtat
     4441 tacgccggcg aaggcgccgc ggaccccgtg aagctcgact tgccgcgcgg cggctacgtc
     4501 gtcaaagcgg cgtatgcgag cagcgagccg gcgagcgccg cggagccctc gggctcgtcc
     4561 gcagagcagc cgcgcgccca ccgcagctgg ctcgcggcga acggccgcgt cgcgtcggcc
     4621 gcggccgcgt tgttgctgat cgttaccgcc gcggtcgtcg ccttgcagtg gcagacaccg
     4681 gtcgccgagc tacg
//
LOCUS       DATLEE010000468         3995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4227760, whole genome shotgun sequence.
ACCESSION   DATLEE010000468 DATLEE010000000
VERSION     DATLEE010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4227760"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VM692_10115"
     CDS             <1..600
                     /locus_tag="VM692_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52566.1"
                     /translation="TWNLNAAISASGVAWVVFDAQVGTRAEELFLAKLDGEPGSLARL
                     SADDGVRSKYPDLALEGERAAITWFDERDGNNEVYMAAGPVADLAQSIDKHARRITIT
                     PGASIGAYVAWNAGQIGLAWSEDSGDNYEVLFQPFDATASPLAAARWLTETRANSMIP
                     AIKPCGDGFVLAWDEVTPGEGGDAHDPSTRAEILFAIVR"
     gene            complement(604..987)
                     /locus_tag="VM692_10120"
     CDS             complement(604..987)
                     /locus_tag="VM692_10120"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52567.1"
                     /translation="MKFAPLGIAATALLAVTSALAHHSPIVFDRTRTVTLTGTVTEFR
                     WGNPHSWIHIDVADKDGKVANWGIEMDPASHLARNGWKSTLVKPGDKVTVVVYPLRND
                     EKGGQYRSITLSDGKQLGQGPEGVI"
     gene            complement(999..1739)
                     /locus_tag="VM692_10125"
     CDS             complement(999..1739)
                     /locus_tag="VM692_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52568.1"
                     /translation="MAGMAQSADAPAQLGALSPENIAKPRPKAPFDLTGTWQHELRGP
                     TSWKFVPQTFELTPEAQVEYDAGRKAAAENKVYRDDIGQCWPAGMPLIMTRVHPWVFL
                     QQPTAIFMIQNFMNSLRIIYTDGRPHSDPDVVVPSWNGESIGRWEGETLVVDTKYFPG
                     HHHWLDQGGASIPASDQLHIVERIRMIDNGETLEFVTTMTDPKTFKGEWATTKRYNRL
                     HDVDIYEVECTPDLNENLPATSSESLVR"
     gene            1974..2291
                     /locus_tag="VM692_10130"
     CDS             1974..2291
                     /locus_tag="VM692_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52569.1"
                     /translation="MNRPILALASVPLLALAAAAYAHNAICECFDNGDDTITCEGGFS
                     DGGKAVGVPVRVFDQSGKVLIDGKMSAQSDYSFPKPKVDFRVEFDAGQGHVVTIDGRD
                     IEK"
     gene            2281..3120
                     /locus_tag="VM692_10135"
     CDS             2281..3120
                     /locus_tag="VM692_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HUQ52570.1"
                     /translation="MKSKPQATALVLGGLLGGLAATAAEAHFQLLYTPEAALNESAAI
                     ELAVVFSHPFDNGFTMNMGAPEELYVVSQRGEGDPRKTDLMQYLEPIKWSGGESQGDA
                     YLVHPPRNVTRSLGDYTFVLKPAPYYEEQEDKYIQQITKTVVNIGGLPGAWDEPLGLP
                     VEIVPLDKPYANWVGGVFRAVVLANGKPVPHAEIEVEFLNHEPQIDERRFDPAGKVTA
                     PQESFSTLSVRADAAGQIIIGLPKAGWWGICALNLDGEEAKHEGKELSLDAVLWVKAA
                     DMK"
     gene            3128..3862
                     /locus_tag="VM692_10140"
     CDS             3128..3862
                     /locus_tag="VM692_10140"
                     /inference="COORDINATES: protein motif:HMM:NF039675.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6162 family protein"
                     /protein_id="HUQ52571.1"
                     /translation="MTAATTTYEVKPAGGRLEGWMLAAASLAVVGTLALYVRLQGSEN
                     EPPKLYDWQISAFDGLAGADQAIYNSLYTVKDEIPLIYDDINMFNEPGVKFRWPNLQD
                     FEDFVLPPFVKDTSWVQQGSLTWTLFEPLAEGEMQGSTMYLGTDGMLPNQGSFLLIIG
                     HVHAGFTNNNAIVIWWNAKNHVEMPESGYRDKLILQGWREVVAYSGDLEVKRLFGDTE
                     PGADDANGEQTTDDVEDVIEGALGDK"
BASE COUNT          718 a         1252 c         1311 g          714 t
ORIGIN      
        1 acctggaact tgaatgccgc gatttcggct tcgggcgttg cctgggtcgt gttcgacgcg
       61 caagtgggaa cgcgcgcaga ggagctgttt ctcgccaagc tcgacggcga gcccggaagc
      121 ctcgcgcggc tcagtgccga cgatggcgtt cgctcgaagt atcccgacct cgcactcgaa
      181 ggcgagcgcg ccgccatcac ctggttcgac gagcgcgacg ggaacaacga ggtttatatg
      241 gccgcgggcc ccgtcgcgga cctggcgcag tcgatcgaca agcacgcccg tcgcatcacg
      301 atcacgccgg gcgcgtcgat cggtgcttac gttgcatgga acgcgggcca gatcgggctc
      361 gcgtggtccg aggacagcgg cgacaactac gaggtgctct ttcagccgtt cgacgcaacg
      421 gccagtccgc tcgccgccgc acgctggctg accgagacgc gcgcgaactc catgatcccg
      481 gccatcaagc cttgcggtga cggcttcgta ttggcgtggg acgaggtcac gccgggcgaa
      541 ggcggcgacg cgcacgaccc gagcactcga gccgaaatcc tgttcgcgat cgttcgttga
      601 tgcttagatc accccttcag ggccttggcc gagctgcttg ccgtcggaca acgtgatcga
      661 gcggtactgg ccgcctttct cgtcgttgcg caacggatag acgacgaccg tgaccttgtc
      721 gccgggcttc accagcgtcg acttccagcc gttgcgcgcc aggtggctcg cggggtccat
      781 ctcgatgccc cagttcgcga ctttgccgtc cttgtctgcg acgtcgatgt gaatccacga
      841 gtgcgggttg ccccagcgga attccgtgac ggtgcccgtc agcgtcaccg tgcgcgtgcg
      901 gtcgaagacg atcggcgagt ggtgcgctag cgccgaggtg acggccagca gggcagtggc
      961 ggcgatcccg agcggtgcaa atttcatggc agtctccgtt agcgaaccag cgactcggac
     1021 gacgtcgcgg gcaagttttc gttcaagtcc ggcgtgcact cgacctcgta gatatcgacg
     1081 tcgtgcagcc ggttgtaccg cttcgtcgtc gcccattcgc ccttgaatgt tttcgggtcg
     1141 gtcatcgtgg ttacgaattc gagcgtctcg ccgttatcga tcatgcggat ccgctcgacg
     1201 atatgcagct ggtcgctcgc cggaatgctg gcgccgccct ggtcgagcca gtggtggtgc
     1261 cccgggaagt acttcgtatc gacgaccagc gtttcgcctt cccagcggcc gatcgactcg
     1321 ccgttccagc tcggcacgac gacgtcgggg tcgctgtggg gccgcccgtc ggtatagatg
     1381 atccgcaggc tattcatgaa gttctggatc atgaagatgg cggtcggctg ttgcagaaag
     1441 acccaggggt ggacccgagt catgatcagc ggcatgccgg ccggccagca ctggccgatg
     1501 tcatcgcggt agaccttgtt ctcggccgcc gctttgcggc cggcgtcgta ctcgacctga
     1561 gcctcgggcg tgagctcgaa ggtctgcggc acgaacttcc aggacgtcgg cccgcgcagc
     1621 tcgtgctgcc atgtccccgt caggtcgaag ggtgccttcg gccgtggctt cgcgatattt
     1681 tccggcgaga gggcgccgag ctgcgccggc gcgtcggcgg actgggccat tccggccatc
     1741 gacgttacgg tagcaagcac cgccgccgcc aagaccctgc atgctcgaac ggtcataaca
     1801 cccccttaag acaggcactt agcgtacccg cggaaccgcc ggctgtgaac cgacgctcgt
     1861 tcaccccgcg ttaagcacgt taacggttag ctaaccgttg atgacttacc gacggtcctg
     1921 ctcttagcga gaatgccacc acttggctgc gactacccca cgaggaatcc accatgaata
     1981 ggccaatttt ggcgctagcc tccgtaccgc tgctcgccct ggcggcggcg gcttatgccc
     2041 ataacgcgat ctgcgagtgc ttcgacaacg gcgacgacac gatcacctgc gagggcggtt
     2101 tcagcgacgg cggcaaggcg gtcggcgtcc cggtgcgcgt gttcgaccag tccggcaagg
     2161 ttctgatcga cggcaagatg agcgcgcaga gcgactatag ttttcccaag ccgaaggtcg
     2221 acttccgcgt cgagttcgat gccgggcagg ggcacgtcgt cacgatcgac ggccgcgaca
     2281 ttgaaaagta agccgcaagc gacggcgctg gtcctcggcg ggctgctcgg cgggttggcc
     2341 gcgacggccg ccgaggcgca cttccagctg ctctatacgc ccgaggccgc cctcaacgag
     2401 tcggcggcaa tcgagctcgc cgtcgtgttc tcgcacccgt tcgacaacgg cttcacgatg
     2461 aacatgggcg cgcccgagga gctctacgtc gtgagccagc gcggcgaggg cgacccgcgc
     2521 aagaccgacc tcatgcagta cctcgagccg atcaaatggt cgggcggcga gagccagggc
     2581 gacgcctacc tcgtgcatcc gccgcgcaac gtcacgcgct cgctcggcga ctacacgttc
     2641 gtgctgaagc ccgcgccgta ctacgaggag caggaagaca agtacatcca gcagatcacg
     2701 aagaccgtcg tgaacatcgg cggcctgccg ggcgcttggg atgagccgct cggcctgccc
     2761 gtcgagatcg tgccgctcga caagccgtac gcgaattggg tcggcggcgt gtttcgcgcc
     2821 gtcgtgctcg cgaacggcaa gccggtgccg cacgcggaga tcgaggtcga gttcttgaac
     2881 cacgagccgc agatcgatga gcggcgcttc gaccccgccg gcaaggtcac ggcgcctcaa
     2941 gaatcctttt cgacgctgag cgtccgcgca gacgcggcgg gtcaaatcat catcggttta
     3001 ccgaaggcag gctggtgggg catttgcgcg ctgaacttgg acggcgaaga ggccaagcac
     3061 gagggcaagg agctctcgct cgacgcggtc ctgtgggtaa aagcggcgga catgaagtga
     3121 gagctcagtg acggcggcga cgacaacgta cgaggtcaag cccgcgggcg gccggctcga
     3181 gggctggatg ctggccgcgg cgtcgctggc cgtcgtcggc acgctggcgc tctacgtgcg
     3241 cctgcaaggc agcgagaacg agccgccgaa gctctacgac tggcagatca gtgcgttcga
     3301 cggcttggcc ggcgccgatc aggctatcta caactcgctg tatacggtca aggacgagat
     3361 cccgctgatc tacgacgaca tcaacatgtt caacgagccc ggcgtgaaat tccgttggcc
     3421 gaatctgcag gacttcgagg atttcgttct gccgccgttc gtcaaggata cgagctgggt
     3481 gcagcaaggc tcgctgacgt ggacgttgtt cgagccgctc gccgaaggcg agatgcaagg
     3541 ttcgacgatg tatctcggca cggacggcat gctgccgaat caaggttcgt tcctgctcat
     3601 catcggccac gtgcacgccg gcttcacgaa caacaacgcg atcgtgatct ggtggaacgc
     3661 caagaaccac gtcgagatgc cggaaagcgg ctaccgcgac aagttgatcc tgcaaggttg
     3721 gcgcgaagtc gtcgcctaca gcggcgattt ggaggtcaag cggctgttcg gcgacacgga
     3781 gccgggcgcc gatgacgcga acggcgagca gactacggac gacgtcgaag atgtgatcga
     3841 aggcgctctc ggagacaagt gatggtgcgg tcaggacgaa cactcgttaa gttgcttttc
     3901 ctcgcgtttg cgacgttcgg tgcgcaaagc gctgccgcga aaatgctgaa ggtcggcatc
     3961 acgctgcacc cgtactacag cttcgtcgcc aacat
//
LOCUS       DATLEE010000469         3109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1270631, whole genome shotgun sequence.
ACCESSION   DATLEE010000469 DATLEE010000000
VERSION     DATLEE010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270631"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VM692_10145"
     CDS             <1..229
                     /locus_tag="VM692_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016390259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HUQ52572.1"
                     /translation="IIGQGGIQTARDALEFVLAGASTVGLGTALFYDPLAPMKVNAGI
                     EEYLRTHRFTSITDLVGGIDPAKALNQAASG"
     gene            255..1043
                     /gene="lpxA"
                     /locus_tag="VM692_10150"
     CDS             255..1043
                     /gene="lpxA"
                     /locus_tag="VM692_10150"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HUQ52573.1"
                     /translation="MTIHPSVILSERAKISAEAEIGPYCIIDGEVEIGPGTIVESHAR
                     IGSRYGRVTIGAKNRIQHGAVLGGPPQDLGYEGGSYTELTIGSHNRIGEYVTINLGTA
                     KGGGVTRVGDHNFIMAYTHIGHDCHIADHVIIVNGAQFAGHVTVEHHANVSGLTGFTQ
                     FIRIGAYAYIAAGAFANKDIVPYTIAEGHWATPRAVNRVGLKRAGFDAGERRNIDTAL
                     RLVLDRSLTIEEVTARIASDCAASPQIEHLLAFIRNSERGIARG"
     gene            1040..1978
                     /locus_tag="VM692_10155"
     CDS             1040..1978
                     /locus_tag="VM692_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HUQ52574.1"
                     /translation="MSKLRTAVVGVGYLGRFHAQKHRLVDGVELVAVCDRDAERGRSV
                     AAEVATNALQDYRELVGRVDAVTIAATTAEHFTLAKFFLENGVHVLVEKPMTRTSAEA
                     AILTKLAAERRLKLQVGHVERFNPALVAARETLTTVRFIECHRLAPFKGRGADVNVIL
                     DVMIHDLDVILSLVDSKPVSASAVGIPVLMDDVDIANARIEFANGAIANVTASRVSTT
                     PQRRFRVFQPNQYVSIDFGNGEVRRVTKDGEWREGASPLREETWNLEKGDALLAETKA
                     FVDSVVNDKPCVVSGTDGLASLELAEMIGADIARRR"
     gene            complement(2416..>3109)
                     /locus_tag="VM692_10160"
     CDS             complement(2416..>3109)
                     /locus_tag="VM692_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUQ52575.1"
                     /translation="LDEIGDMSLALQTRLLRVLAESEFFRVGGQTAIRVDVRVIAATH
                     QDLAHGAAAGSFREDLYHRLNVMRIEVPPLRQRREDIRELSRFYLDQAANELGVAPKT
                     LSADAMEALQAYDWPGNVRELVHVCRRLTVTAAGREIVGQDLPAELGGVASQAGADWT
                     AGLSQWAEAQLAIAAPPPLLIDALPAFERTLILTALRRARGKRLEAAKLLGWGRNTLT
                     RKIKELGLEGRV"
BASE COUNT          531 a         1070 c         1029 g          479 t
ORIGIN      
        1 gatcatcggc cagggcggca tccagaccgc acgcgacgcg ctcgagttcg tgctcgccgg
       61 cgcgtcgacc gtcgggctcg gcaccgcgct cttctacgat ccgctcgcgc cgatgaaggt
      121 gaacgccggc atcgaagagt atttgcgtac gcatcggttc acgagcatca cggacctcgt
      181 cggcggcatc gatccggcca aagcactgaa ccaagccgcg agcggctgag acgcgccgca
      241 gcgtaggacc gtcgttgacc attcacccga gcgtcatctt gagcgagcgc gccaagattt
      301 ccgccgaggc ggagatcggc ccgtattgca tcatcgacgg cgaggtcgaa atcggccccg
      361 gaacgatcgt cgagtcgcat gcgcgcatcg gtagccgcta cggccgcgtg acgatcggcg
      421 ccaagaaccg catccagcat ggcgccgtgc tcggtgggcc gccccaagac ctgggctatg
      481 aaggtggctc gtacaccgag ctgacgatcg gctcccataa ccggatcggc gagtacgtca
      541 cgatcaacct tggcacggcg aagggcggcg gcgtcacgcg cgtcggcgat cacaacttca
      601 tcatggccta cacgcacatc ggccacgatt gccacatcgc cgatcacgtc atcatcgtca
      661 acggcgcgca attcgcgggc catgtcaccg tcgagcacca cgccaacgtg agcggtctga
      721 ccggcttcac gcagttcatt cggatcggcg cgtacgcgta catcgccgcc ggcgcgttcg
      781 cgaacaaaga catcgtgccg tacacgatcg ccgagggtca ctgggcaacg ccgcgcgccg
      841 tcaatcgcgt gggtctcaag cgtgcgggat tcgacgccgg cgaacgccgc aacatcgaca
      901 ccgcgctccg gctcgtcctc gatcgctcac tgacgatcga ggaagtgacg gctcgcatcg
      961 cgagcgactg cgccgcaagc ccgcagatcg agcacctgct cgcgttcatc cgcaactccg
     1021 agcgcgggat cgcacgcggg tgagcaagct gcgcaccgcc gtcgtcggcg tcggttatct
     1081 cggccggttt cacgcgcaga agcaccgact cgtcgacggc gtcgagctcg tcgcggtttg
     1141 cgatcgcgac gccgagcgcg gccgcagcgt cgcggccgag gtcgcgacga atgcgttgca
     1201 ggactaccgc gagctcgtag gcagagtcga cgcagtgacg atcgccgcga cgacggccga
     1261 gcacttcacg ctcgcgaagt tcttcctcga gaacggtgtg cacgtgctgg tggagaaacc
     1321 gatgacgcgc acgagcgcgg aagccgcgat tctcacgaag ctcgccgccg agcggcggct
     1381 caagctgcag gttggccacg tcgagcgctt caatcctgcc ctcgtcgcgg ctcgcgagac
     1441 gttgacgacg gtgcgcttca tcgaatgtca tcgtctggcg cccttcaaag gtcgcggcgc
     1501 cgacgtcaac gtcatcctcg acgtcatgat tcatgatctc gacgtgattc tgtcgctcgt
     1561 cgactcgaag cccgtcagcg cctcggcggt cggtatcccc gtcctcatgg acgacgtcga
     1621 catcgctaat gcgcgcatcg agttcgcgaa cggcgcgatc gcgaacgtca cggcgtcgcg
     1681 cgtgtcgacg accccgcagc ggcgttttcg cgtgttccaa ccgaatcagt atgtttcaat
     1741 cgacttcggc aacggcgaag tgcggcgcgt gacgaaggac ggcgaatggc gcgagggcgc
     1801 gtcgccgctg cgcgaggaaa cctggaacct cgagaaaggc gacgcgttgc tcgccgaaac
     1861 caaagccttc gtcgactcgg tcgtcaacga caagccatgc gtggtgtcgg gtaccgacgg
     1921 cctcgcttcc ctcgagctcg ccgagatgat cggcgccgac atcgcccgcc gccgctaacg
     1981 accgggggcg gctatcgact gccgccgcta gcgctgccgc cgaagtcacg agctatgcct
     2041 cacatggccg ctgctgcttg cggtggaaca gcgcacgcac cgagcgaggc gcaagcattt
     2101 cgcgcggcga ggcgcgtttc gtcgtgcgag cgggctgcgc tgcgggtgag tgcggccgcg
     2161 tgaggcgtgt ttcgtgactc ggctcggagg ccgcgcgcaa gtgggccgag aacactcgat
     2221 gcgtcttccg aatggcgagc agtcccgggc cccgatgcaa agctttgaat ggcacgcgcg
     2281 gcccacgccg ctcgcaagag cgccggtgac aaaccggcct tcgagccgag ccgcgagaca
     2341 cgcctcgcgc ggccgagccc accgcgcgag gagccgagcg cacacgacgc gcgatctgcc
     2401 tgcggacgaa gcgctctaga cgcggccttc gaggccgagc tctttgatct tgcgcgtcag
     2461 cgtgttgcgg ccccagccga gcagcttagc ggcctcgagt cgcttgccgc gggcgcggcg
     2521 caacgccgtc aagatcagcg tccgctcgaa agccggcagc gcatcgatca acagcggcgg
     2581 cggcgcggcg atcgcgagct gcgcctcggc ccattgcgag aggccggccg tccagtcggc
     2641 gccggcctgg cttgcgacac cgccgagctc ggccggcaag tcttggccga cgatctcgcg
     2701 gcccgcggcc gtcacggtca accgccggca gacatggacg agctcgcgca cgttgccggg
     2761 ccagtcgtac gcttgtagcg cctccatcgc gtcggcgctc agcgtcttcg gcgccacgcc
     2821 gagctcgttg gcggcctggt cgagatagaa gcgcgacagc tcgcggatgt cttcgcgccg
     2881 ctgccgcagc ggcggcacct cgatgcgcat gacgttcagg cggtgataca gatcctcgcg
     2941 gaaacttccc gccgcggcgc cgtgcgcgag gtcctggtgc gtggccgcga tcacgcgcac
     3001 gtcgacgcgg atcgcggtct gaccgccgac gcggaagaac tcgctctccg cgagcacgcg
     3061 caacaaccgc gtctgcagcg ccagcgacat gtcgccgatc tcgtcgagg
//
LOCUS       DATLEE010000470         4295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2015174, whole genome shotgun sequence.
ACCESSION   DATLEE010000470 DATLEE010000000
VERSION     DATLEE010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015174"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1882
                     /locus_tag="VM692_10165"
     CDS             194..1882
                     /locus_tag="VM692_10165"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ52576.1"
                     /translation="MSTKIISLLVPFALAVSSPSLAQPAPQPPSQPCPAVRDVLLTNG
                     KIHTMNTSNAVVDSVRIVGDRFAEVGTNRLERSRCTRTIDLEGRTAVPGLIDNHNHIV
                     LLGLRPGHDTRLENAKSIDDVLGVLRAKAATLPNGEWITSIGGFDINQFVPPPGMPRF
                     PTLAELDSVTPNHPVYIQMSFSGPSVTNTLGRQFFTQQGIEVGADGAIAGGFQLPNPT
                     TRALHELRQRQTLADQKRGTQDAMRYAASLGLTTHLDEGGFPATGTATDAAAHMDVYR
                     AYDALLALYAEGAMTQRIRINFLHMETDIATPQLAARLNNVFPRYGADMLKVLGIGEF
                     TAGASPILAEASDVWRAGTKRVAEAGWRNENHSLTPGDFKVIIDGWAQIDASLPAPGI
                     KQSRWVVAHAPFITQEYIDKLKALGGGVSVLGGWRYISGTAQQNGPPFKLLADSGIPI
                     GMSSDGMQISPMNPWLGLYYVVTGKNARGDVINAGQTLPRADALRLYTAANGWFLNEE
                     SLLGSIEPGKYADLAVLSADYFDTRAVSDEAIKDVRSMLTLVGGQIVHGDADKL"
     gene            complement(1909..3084)
                     /locus_tag="VM692_10170"
     CDS             complement(1909..3084)
                     /locus_tag="VM692_10170"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ52577.1"
                     /translation="MRIYLGLASALLLAGQPAQAQDSRASPPLPSLPQVYETADLKIR
                     VVQVADGLANPWSLAWLPNGDLLITERAGRLRIVRNGVLDREPIAGVPEVRITALGGL
                     LDVALHPNFATNRLLYLSYTKGGEGNSATTAITRARLDGMKLVDANEIFVADNWSNSP
                     TNFGGRMVFGRDGMLYTAIGERQEQERAQNTMLHGGKVLRLRDDGTAPADNPFVGKPG
                     YLPEIYSVGHRSPQGLVFHPVTGDLYENEHGPLGGDEINIVRAGKNYGWPLVTYGKNY
                     DGTMVTEYQTRDGYESPFMFWVPSIAISGLSFYTGDKLAPWKGNAFVGSMMQGRTRAT
                     GHVQRITFAENGQPIQREPILKELRQRIRDVRPGPDGLLYVLTDQNPGVLLRIEPAE"
     gene            3154..3531
                     /locus_tag="VM692_10175"
     CDS             3154..3531
                     /locus_tag="VM692_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52578.1"
                     /translation="MVSKPWAWLTGVAWLFAALTNAHGHIHFCFDGKEPPAAVHLTDS
                     SDHDHELPGHDASGDHDDLDVDLPNQALAKACKHDLAVIAPVLVWTAGFVTPSTTTPI
                     AADPAPPVPDTLYYRPPSRAPPR"
     gene            3628..>4295
                     /locus_tag="VM692_10180"
     CDS             3628..>4295
                     /locus_tag="VM692_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUQ52579.1"
                     /translation="MIVSARHASIVACAAIGIGLTSASLAAPLTLAEAIERTIANNPE
                     LRVYAPRLRAAEQHANAAALRPPFELITEAQDVFGTGRASGFDSTESTIELSQVIELG
                     NKRGRRVDAAMAGSALIDAERAVAELDVLAEVTRRLIHVAADQEHLAVTQRATALADE
                     TVAAATARVAAARAPDVELRRARVTRARAGVEQEHAEHELLTSRRQLAAMWGDSEPAF
                     DDVSA"
BASE COUNT          717 a         1509 c         1403 g          666 t
ORIGIN      
        1 ctcgagcgac agcatcgggc cgtcgagctg cgccacggcc gttgctgcgc acagcaacgg
       61 caagaaggca aaaccggtgg aaatcagccg aaccattgcg cctcgagctc tcgccggctt
      121 tcgcggccgc gtgaggatag cctaaagtac ctaccaagcg tctcgatcga agaccattca
      181 ggggggcctg gtcgtgtcga ccaaaataat ttcgctgctc gtgccgttcg cgctcgccgt
      241 ctcgtcgccg tcgctcgcgc agccagcgcc gcaaccgccg tcgcagccct gccccgccgt
      301 gcgcgacgtg ctgctcacga acggcaaaat tcacacgatg aacacgagca acgcggtcgt
      361 cgattcggtg cgcatcgtcg gcgatcgctt cgcggaggtc ggcacgaacc ggctcgaacg
      421 gtcgcgttgc acgcggacga tcgacctcga aggccgcacg gccgtgccgg ggctcatcga
      481 caatcacaat cacatcgtct tgctcggctt gcgacccggt cacgacacgc ggctcgagaa
      541 cgccaagtcg atcgacgacg tgctcggcgt cctgcgtgca aaggccgcga cgttgccgaa
      601 cggcgagtgg atcacgtcga tcggcggctt cgacatcaat cagttcgtgc cgccgcccgg
      661 catgccgcgt ttcccgacgc tcgccgagct cgactcggtg acgccgaacc atcccgtcta
      721 cattcagatg tcgttctcgg gcccgtccgt gacgaacacg ctcggccggc agttcttcac
      781 gcagcaaggc atcgaggtcg gagccgacgg cgcgatcgcg ggcggctttc agttgccgaa
      841 cccgacgacg cgcgcgctgc acgagctgcg ccagcggcag acgctcgccg accaaaagcg
      901 cggcacgcaa gatgccatgc gctacgcggc cagcttgggg ctcacgacgc acttggacga
      961 gggcggcttc ccggccacgg gcacggcgac cgacgcggcg gctcatatgg atgtctaccg
     1021 cgcctacgat gcgctgctcg cgctgtacgc cgaaggcgca atgacgcagc gcattcgcat
     1081 caacttcctg cacatggaga ccgacatcgc gacgccgcag ctcgcggcgc ggctcaacaa
     1141 tgtgtttccg cgctacggcg ccgacatgct caaggtgctc ggcatcggcg agttcacggc
     1201 gggcgcttcg ccgatcctcg cggaagcgag cgacgtgtgg cgcgccggca cgaaacgcgt
     1261 cgccgaagcc ggctggcgca acgagaatca ctcgctgacg ccgggcgact tcaaagtcat
     1321 catcgacggt tgggcgcaaa tcgacgcgtc actgccggcg cccggaatca agcagagccg
     1381 ctgggtcgtc gcgcacgcgc cgttcatcac gcaggagtac atcgacaagc tgaaagcgct
     1441 cggcggcggc gtcagcgtgc tcggcggctg gcgctacatc agcggcacgg cacagcagaa
     1501 cggcccgccg ttcaagctgc tcgccgacag tggcatcccc atcggcatga gctccgacgg
     1561 catgcagatc tcgccgatga atccgtggct cggcctctac tacgtcgtga cgggcaagaa
     1621 cgcgcgtggc gacgtcatca acgcgggcca gacgctgccg cgcgccgacg cgctgcgcct
     1681 gtacactgcg gccaacggct ggttcttgaa cgaagagagc ctgctcggca gcatcgagcc
     1741 cggcaagtac gcggacctcg ccgtgctatc ggccgactac ttcgacacgc gcgccgtctc
     1801 cgacgaggcg atcaaagacg tacgttcgat gttgacgctc gtcggcgggc agatcgtgca
     1861 cggcgacgcg gacaagctgt agttgccggg cgccgtgcag ggacgcgact actccgccgg
     1921 ctcgatgcgc agtagcacgc cggggttctg atccgtcagc acgtacagca agccgtcggg
     1981 gccgggccgc acgtcgcgga tgcgctggcg cagctccttg agtatcggct cgcgctggat
     2041 cggttggccg ttctcggcga acgtgatgcg ctgcacgtgg cccgtcgcgc gcgtgcggcc
     2101 ttgcatcatc gagccgacga acgcgttgcc cttccacggc gcgagcttat cgcccgtgta
     2161 gaagctcaag cccgagatcg caatcgacgg cacccagaac atgaacggcg attcgtaacc
     2221 gtcgcgcgtc tgatactcgg tcaccatcgt gccgtcgtag ttcttgccgt acgtcacgag
     2281 cggccaaccg tagttcttgc cggcgcgcac gatgttgatc tcgtcgccgc cgagcgggcc
     2341 gtgctcgttc tcgtaaagat cgccggtcac ggggtgaaag acgagacctt gcgggctgcg
     2401 atggccgacg gaataaattt ccggcagata ccctggcttg ccgacgaacg gattatcggc
     2461 cggcgccgtt ccgtcgtcgc gcagccgcag caccttgccg ccgtgcagca tcgtgttctg
     2521 cgcccgctcc tgctcctgcc gctcgccgat cgcggtgtac agcatgccgt cgcggccgaa
     2581 taccatgcgg ccgccgaagt tcgtcggcga gttgctccag ttatccgcga cgaagatttc
     2641 gttcgcgtcg acgagtttca tgccgtcaag gcgcgcgcgc gtgatcgccg tcgtcgcact
     2701 gttgccttcg ccgcctttcg tgtagctcaa atagaggagt cggttcgtcg caaaattcgg
     2761 gtgcaacgcg acgtcgagca aaccgccgag cgccgtgatg cgcacctcgg gcacgccggc
     2821 gatcggctcg cgatcgagca cgccgttgcg cacgatgcgc aggcgaccgg cacgctccgt
     2881 gatcagcaga tcgccgttcg gtaaccacgc gaggctccac gggttcgcga gaccgtcggc
     2941 gacttgcacg acgcggatct tgaggtcggc cgtctcgtag acctgcggca gcgacggcaa
     3001 cggcggcgag gctcgggagt cctgggcctg cgcgggctgc ccggccaaca gcagcgcgct
     3061 cgccaagccg agatagatcc gcatcgcccg ctcccgtcac gccgatggcc ggcaaggata
     3121 gccactgtag ccgcaagccg ctatcctcgg cgcgtggtct cgaaaccttg ggcttggctg
     3181 accggcgtcg cgtggctgtt cgccgcgctg accaacgcgc acggccacat ccatttctgt
     3241 ttcgacggca aagagccgcc ggcggccgtc cacctgacgg acagctccga ccacgatcac
     3301 gagcttccgg gtcacgacgc atccggcgac cacgacgacc tcgatgtcga tttgccgaat
     3361 caagccctcg cgaaagcgtg caagcacgat ctggccgtca tcgcgcccgt gctcgtgtgg
     3421 accgcgggct tcgtgacacc gagcacgacc acgccaatcg cggcggaccc ggccccgccg
     3481 gtgcccgaca cgctctacta ccgcccaccc tcgcgcgcgc cgccgcgctg atcctcgacg
     3541 ctccgtcgcc gctgcgctgc gcgcagccgt tcgattgccc gcgggcggcg cttcggcgcc
     3601 gcgccgctca gccttctggg aggctgcatg atcgtttcag cgaggcacgc atcgatcgtc
     3661 gcgtgcgccg ccatcggcat tggcctgacg agcgcctcgc tcgcggcccc attgacgttg
     3721 gccgaagcca tcgagcgcac gatcgccaac aatcccgagc tgcgcgtcta tgcgccgcgc
     3781 ttgcgcgccg ccgagcaaca cgcgaacgcg gcggcgctgc ggccgccatt cgagctcatc
     3841 accgaagcgc aggacgtgtt cggcacgggc cgcgcgagcg gcttcgatag caccgagtcg
     3901 acgatcgagt tgtcgcaggt catcgagctc ggcaacaagc gcggccgccg agtcgacgcg
     3961 gccatggccg gctcggcgct gatcgatgcc gagcgcgccg tcgccgagct cgacgtgctt
     4021 gccgaggtca cgcggcggct gatccacgtc gcggccgatc aggagcacct cgcggtcacg
     4081 caacgcgcca cggcgctcgc ggacgagacg gtcgcggcgg cgacggcgcg cgtcgcggcc
     4141 gcacgcgcgc ccgacgtcga gctgcggcgc gcacgcgtca cacgcgcacg cgccggcgtc
     4201 gagcaggagc acgccgagca cgagctgctc acgtcgagac ggcagctcgc ggcgatgtgg
     4261 ggcgacagcg agcctgcgtt cgacgacgtc agcgc
//
LOCUS       DATLEE010000471         2362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8462809, whole genome shotgun sequence.
ACCESSION   DATLEE010000471 DATLEE010000000
VERSION     DATLEE010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8462809"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1661)
                     /gene="serA"
                     /locus_tag="VM692_10185"
     CDS             complement(75..1661)
                     /gene="serA"
                     /locus_tag="VM692_10185"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HUQ52580.1"
                     /translation="MARILVSDKLDKQGLEILLAAPGLEVDNNPGLPADELKKIIGNY
                     EGLVIRSGTKVTADLLSAADNLKIVGRAGIGVDNVDVEAASKRGVIVSNTPGGNNVTT
                     GEHAISMMLACARDIPQANATLRAGQWKRNDFIGVEVTGKTVGVIGLGNIGAIVADRA
                     KGLRMNVIGYDPFVTPEAAARLGIDCVTLDELYARADFISIHTPLTNDTRGLIGEAVI
                     AKMKKGVRIVNCARGGIVDDKALLAALNSGQVAGAALDVFGSEPPAKDDALVGHPKVV
                     ATPHLGASTGEAQLNVAIAVAEQIRDFFVDGTIRNAINVPSLSAEAAGAVAPFIALGE
                     KIGNMHAQLSNCAPNEIVIAYNGDVAGLDIRPVNAAVLKGLLSSVLDVPLNAVNAPFL
                     AKDRGVKIVEVKNRDTKSFTNSISVTFKRGSESRVIEGAVFGSKHARIVRFGDFFLEA
                     EPAGNILILHNRDVPGVVGRLGTYLAQLNINIAGLSLGRETQGGQAVAFYHVDSALDA
                     AQLEQVRALQDVTGAQMVRL"
     gene            complement(1674..>2362)
                     /locus_tag="VM692_10190"
     CDS             complement(1674..>2362)
                     /locus_tag="VM692_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HUQ52581.1"
                     /translation="DTDTLLICDGITSVGVLDCPMDATGIDVQVTGSQKALMLPPGLG
                     LIALSEKAWDASKRSKLPRFYFDLAKAKKALDEDTSAYTPAVSLLFGLDAVYRSVEQN
                     GGWSEVYRRHRILAAAARAGIQAIGLRLLAPTAPSPATTGIWVPDGVDGGKLTKYLRD
                     VLGVTIAGGQGKLKGKILRLAHIGYADSFDVVVGLSAIEMALRHFGHDAAFGKGPGAA
                     GEALLEMYPN"
BASE COUNT          381 a          765 c          768 g          448 t
ORIGIN      
        1 ggaagtacgc cttcgctgcc gggcccgaaa tccgccttgc agctggaccc ttgcgcgcgg
       61 tctgatgaag acttctacag ccgaaccatt tgcgcgccgg tcacgtcctg taacgcgcgc
      121 acctgttcga gttgcgcggc gtcaagcgcc gaatccacat gatagaacgc aacggcctgt
      181 ccaccctgtg tctcgcggcc gagcgaaagg ccggcaatgt tgatgttgag ctgcgcgaga
      241 taggtgccga gacggcccac tactcccggc acgtcgcggt tgtgcaggat gaggatgttc
      301 cctgccggct ccgcttcgag gaagaagtcg ccaaaacgga cgatcctcgc gtgcttgctg
      361 ccgaagacgg cgccctcgat tacgcgcgac tcgctgccgc gcttgaatgt caccgagatg
      421 gaattcgtga acgacttggt gtcgcggttc ttcacctcga cgatcttgac gccgcggtcc
      481 ttggcaagaa acggcgcgtt gactgcgttc agcggcacgt cgagcacgga cgagagcagg
      541 cccttcagca ccgccgcatt gacggggcgg atgtcgaggc cggcgacgtc gccgttgtaa
      601 gcgatcacga tctcgttcgg cgcgcagttc gaaagctgcg cgtgcatgtt gccgatcttc
      661 tcgccgagcg cgatgaacgg cgcgacggcg ccggccgctt cggccgacag cgaaggcacg
      721 ttgatcgcgt tgcggatcgt tccgtcgacg aagaagtcgc ggatctgctc ggccaccgcg
      781 atcgccacat tcaactgggc ctcgccggtc gatgcgccga gatgaggcgt ggcgacgact
      841 ttcgggtgtc ccacgagcgc gtcgtccttg gcgggcggct cgctgccgaa cacgtcgaga
      901 gctgcgccgg cgacctgtcc ggagttgagc gccgccagca gcgccttgtc atccacgatg
      961 ccgccgcgag cgcagttcac gatgcgcacg cccttcttca tctttgcgat caccgcttcg
     1021 ccgatgaggc cgcgcgtgtc gttggtaaga ggcgtgtgga tcgagatgaa gtcggcgcgt
     1081 gcgtagagct cgtcgagcgt gacgcagtcg atgccgaggc gcgcggcggc ttccggcgtc
     1141 acgaatggat cgtagccgat cacgttcatg cgaaggcctt tggcacgatc ggcgacgatg
     1201 gcgccaatgt tgccgagccc gatcacgccg accgttttgc cggtgacttc cacgccgatg
     1261 aagtcgttgc gcttccactg gcccgcgcga agcgtcgcgt tggcctgggg aatgtcgcgg
     1321 gcgcacgcga gcatcatcga gattgcgtgc tcgcccgtcg tcacgttgtt gccgcccggc
     1381 gtgttcgaga cgatgacgcc gcgcttgctc gccgcttcga cgtcgacgtt gtcgacaccg
     1441 ataccggcgc gaccgacgat cttcagattg tcggcggccg acagcagatc ggccgttacc
     1501 ttcgtgccgc tgcggatcac gaggccttcg taattgccga tgatcttctt cagctcatcg
     1561 gccggaagac ccgggttgtt gtccacttcg agcccggggg ccgccagaag tatttcgagg
     1621 ccctgcttgt cgagcttgtc ggaaaccaga attcgagcca cgttcgccct ctctcagttg
     1681 ggatacatct cgagcagcgc ttcgcctgcc gcgccgggtc ccttcccgaa cgccgcgtcg
     1741 tgtccgaagt gccgtagtgc catttcgatg gccgaaagcc cgaccacgac gtcgaagctg
     1801 tcggcgtaac cgatgtgcgc aaggcgaagg atctttccct tcagcttgcc ctgaccgcct
     1861 gcgatggtga cgccaagaac gtcgcgcagg tatttcgtga gcttgccgcc atcgacgccg
     1921 tcgggaaccc agattcccgt cgtcgccgga cttggtgccg tcggcgcgag aagccgaagc
     1981 cctatcgcct ggatgccggc gcgcgctgcc gccgcgagga tgcggtggcg gcggtacacc
     2041 tcgctccatc cgccgttctg ttcgacgctg cggtaaaccg cgtcgagccc gaacaggagc
     2101 gacacggcgg gcgtgtacgc cgacgtgtct tcatcgagcg ccttcttcgc cttcgcaagg
     2161 tcgaagtaga agcgcggcag cttcgagcgc ttgctcgcgt cccacgcctt ttcgctgagc
     2221 gcgatgaggc cgaggcccgg cggcagcatg agcgccttct gcgagcccgt cacctggacg
     2281 tcgatgccgg tggcgtccat cggacagtcg agcacgccga ccgacgtgat gccgtcgcag
     2341 atgagcaacg tgtcggtgtc ct
//
LOCUS       DATLEE010000472         5714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2765382, whole genome shotgun sequence.
ACCESSION   DATLEE010000472 DATLEE010000000
VERSION     DATLEE010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2765382"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VM692_10195"
     CDS             <1..736
                     /locus_tag="VM692_10195"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUQ52582.1"
                     /translation="IAGHSRELPGFPNTGVFASNKAWDAGVRTMADMKGRVIGLTTIG
                     STNHYAVARIGEKYGFDVNENHLVPAQGLGNLAAMLMGNQVDVASGAGTAFMGLIVKG
                     QAHVIGWAGDEISWQSTGLASHPRVIKEKRAALQAIVDTYERGAAEYSAVLLDKNGDG
                     TYKDPAKAEELLALISRWTSVPPAAIAQSLAYIDSRLDVGSIYEMVDWYRGQRMIEKE
                     TDPAKFIDLTFVEGHVNLPAAMRAGH"
     gene            740..1516
                     /locus_tag="VM692_10200"
     CDS             740..1516
                     /locus_tag="VM692_10200"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ52583.1"
                     /translation="MEIALRHITHRFGDLTALDDVSLHAHEGEIVAIIGPSGCGKSTL
                     LGIAGGILKPTAGEVVLRGVPAADTLNEITFIFQDFSLLPWQSVRDNIAFVLKHHKLG
                     AAEMERRVANALERTGLSDFATALPKQLSGGMRQRVGIARALAVRPAVFLMDEPLSAL
                     DAQTRELLLDDILALWERDHPTILYVTHNFQEAIRIADRVCVLSRRPGRVREIIDIPI
                     PHSQRLQPQHQAKVAELGQKLWALIKEEAIVADRELVAHV"
     gene            1509..2336
                     /locus_tag="VM692_10205"
     CDS             1509..2336
                     /locus_tag="VM692_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52584.1"
                     /translation="MSETSAAAAGSDAPARDRKLTFRGGGFVIEQRTWISVVAFLALV
                     GIWQLATGSGLVSPIFLPSPMRVLRALYDLAITGDLWRHMSASLVRIGYGWLIGSLCG
                     IALGLTIGLFTVARAAGLPLVSALFPIPKIALLPLLILWLGIGEGSKVATIALSVFFP
                     TAIATFSGVDSVPRNLIRMAQSFNVPLWRIVSQVLIPGALPGMLAGFRISIAAALLVL
                     VAAEMIGAQYGIGAFLILTGNMMQTDRLMAGVVVVSVLGLAMSMLISAVERRLLRWR"
     gene            complement(2460..3035)
                     /locus_tag="VM692_10210"
     CDS             complement(2460..3035)
                     /locus_tag="VM692_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HUQ52585.1"
                     /translation="MKRFALLAPLALFSLAPLSANALLITPSTPICGSATCLLMTGNQ
                     TSQAVINDVIGDVLGSSIEIYKQNVGGPEVGLFAGSYTTSFYNTAADPKDATIVYNGG
                     SAISQATHLLVKDGNHSPAWYLFKLSWNGTETITLNDFWPGSGAISHVTIYGGTAGTT
                     TTTTQVPEPATMGLLGAGLLAFGFMRRRKAA"
     gene            complement(3276..4949)
                     /gene="ilvD"
                     /locus_tag="VM692_10215"
     CDS             complement(3276..4949)
                     /gene="ilvD"
                     /locus_tag="VM692_10215"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HUQ52586.1"
                     /translation="MTRKLRSNLEEGSVPWSGRRAQWRALGLTDDDMQKPKIAIVNSS
                     SELSSCFSHLDGLADELKVAIRAAGGVPFEIRTAAPSDFITSAGRRGAYILPTRDLIA
                     ADIEVQVEGALLDGMVCLASCDKTTPGQLMAAGRLNIPTIVVVCGYQKAGVYRGRHTD
                     IEDVFLKTGYLTSGAVTIEEVKGMADVAIQSPGVCQGMGTANSMHVVCEALGMTLPGS
                     APVAAGSAKMHAHLRAAGARIVEMVWQDVKPRDIMTAGAVRNAVQMVLAVSGSINCVK
                     HLQAIATEAEADVDVYQAFADHAERVPLLAAVKPNGDGHIEDFEAAGGARAILKRLER
                     QLDLDARSVSGETLREILAATTVTDERTIATLDKPFSRRPAIVIVRGSLLPGGGIVRL
                     GGTGERMLEFRGKANIFHSRDEALAGIKAGHVKPGDVVVLRGLGVIGGPGMAMTSAVI
                     FALDGAGLLDRVAAITEGQLSGLVNNGLVVGEASPEAAAGGPLAYVENGDVISIDVRR
                     RAVDLEVSEDVLVERRKTPRSYGAQDQRGWLGVYQRTVAPVHEGAVLKR"
     gene            5012..>5714
                     /locus_tag="VM692_10220"
     CDS             5012..>5714
                     /locus_tag="VM692_10220"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52587.1"
                     /translation="MRKPGRLNLKKIAESAQPRRTRADVKALLDAAPEVVFARCRSPR
                     YWPIWLFAFWLRLTAALPWRVAIRSHKLLGRMLGRLSGRSRRVVRRNLELCFPELGSA
                     EIRRIIKANCANVGAFFAELAFAWYGDPERRSRLFRFEGLEHLEAAIAKGKGALLYAG
                     HFTPIEICAPMLKSRIPLYAFMFKPRRNPLMNALQNRGRGRYAHVSVTNDDVRELLAL
                     LARNAVIWYSPDQARV"
BASE COUNT          933 a         1860 c         1878 g         1043 t
ORIGIN      
        1 gatcgcgggg cacagccgcg agttgccggg gtttccgaac acgggcgtgt tcgcgtcgaa
       61 caaagcgtgg gacgccggcg tgcgtacgat ggccgacatg aagggccgcg tgatcggcct
      121 tacgacgatc gggtcgacga atcactacgc cgtcgcgcgc atcggcgaga agtacggctt
      181 cgacgtcaac gagaaccacc tcgtgcccgc gcaagggcta ggcaatctcg ccgcgatgct
      241 gatgggcaac caggtcgacg tcgcgtcggg cgccggcacc gcgttcatgg gtctcatcgt
      301 caaaggccag gcgcacgtca tcggctgggc cggcgacgag atctcgtggc aatcgactgg
      361 gcttgcgtcg cacccgcgcg tcatcaagga gaagcgcgcg gcgctgcaag cgatcgtcga
      421 cacctacgag cgcggtgccg ctgaatactc ggccgtgcta ctcgacaaga acggcgacgg
      481 tacctacaag gacccggcca aagcggaaga gctgctcgcg ctgatctcgc gctggacgtc
      541 cgtgccgcca gccgccatcg cgcagtcgct ggcgtacatc gacagccgcc tcgacgtcgg
      601 cagcatctac gagatggtcg actggtaccg agggcaacgc atgatcgaga aagagaccga
      661 tccggccaag ttcatcgacc tgaccttcgt cgagggacac gtcaacctcc ccgcggccat
      721 gagggcgggg cactagcgca tggaaatcgc gctaaggcac atcacgcatc ggttcggtga
      781 cctgacggca ctcgacgacg tttcgctgca cgcgcacgaa ggcgagatcg tcgcgatcat
      841 cggcccgtcg ggctgcggca aatcgacgct gctcggcatc gccggcggca tcttgaagcc
      901 gacggccggc gaggtcgtgc tgcgcggcgt gccggcggcc gacacgctga acgagatcac
      961 cttcatcttc caggatttct cgctgctgcc gtggcagagc gtgcgcgaca acatcgcgtt
     1021 cgtattgaaa caccacaagc tcggcgcggc cgagatggag cggcgcgttg ccaacgcgct
     1081 cgagcgcacg ggcctgagcg acttcgccac cgccctgccg aagcagctat cgggcggcat
     1141 gcggcagcgc gtcggaattg cgcgcgcgct cgcggtccgg ccggccgtgt ttctaatgga
     1201 cgagccgctg tcggcgctcg acgcgcaaac acgcgagctg ctgctcgacg acatcctcgc
     1261 gctctgggag cgcgatcatc cgacgatctt gtacgtcaca cacaacttcc aggaagcgat
     1321 tcgcatcgcc gatcgcgttt gcgtgctgtc gcggcggccc ggccgcgtgc gcgagatcat
     1381 cgatattccg attccgcact cgcagcgctt gcaaccgcag catcaagcca aggtggccga
     1441 gctcggtcag aagctttggg cgctgatcaa agaggaggcg atcgtggccg accgcgagct
     1501 cgttgcccat gtctgagacg agcgcggcag ccgcagggag cgacgcgccg gcgcgcgatc
     1561 gcaagctgac gttccgcggc ggcggcttcg tcatcgagca gcgcacctgg atcagcgtgg
     1621 tggcattcct cgcgctcgtc gggatttggc agctggccac cggtagcggc ctcgtcagcc
     1681 cgatattttt gccgtcgccg atgcgcgtgc tgcgtgcact ctacgatctc gcgatcacgg
     1741 gcgatctgtg gcggcacatg tcggcctcgc tcgtccgtat cggttacggt tggttgatcg
     1801 gctcgctgtg cggcatcgcc ctcggcttga cgatcggcct gttcacggtc gcgcgcgccg
     1861 ccggcttgcc gctcgtctcg gcgctgttcc caattccgaa gatcgcgctg ctgccgctgc
     1921 tgatcctctg gctcgggatc ggcgagggct cgaaggtcgc gacgatcgcg ctcagcgttt
     1981 tcttcccgac ggcgattgcg accttcagcg gcgtcgactc cgtgccgcgc aacttaattc
     2041 gcatggcaca gagcttcaat gtgccgttgt ggcgcatcgt ctcgcaggtg ctgatccccg
     2101 gcgcgctgcc cggcatgctc gccggcttcc gcatctcgat cgccgcggca ttgctcgtgc
     2161 tcgtcgccgc cgagatgatc ggcgctcaat acggcatcgg cgcctttctg atcctgacgg
     2221 gcaacatgat gcagacggac cggctcatgg ccggcgtcgt ggtggtctcc gtgctcggcc
     2281 tcgcaatgag catgctgatc tcggccgtcg agcggcgttt gctgcgctgg cgctagcgcc
     2341 tgagcttgga gcccaaagcg cgctcgcgtc catgtcgtcc gcaagatgtc ctgcgtcagc
     2401 gaaagaaaaa ggccggcgac tttcgtcggc cggccctgag acggtgtcgt tgttcgggac
     2461 taggcggcct tgcggcggcg catgaacccg aacgcgagca gcccggcgcc gagcaagccc
     2521 atcgtggcgg gctccggcac ttgagtcgtc gtcgtcgtcg tgccggcggt gccgccgtag
     2581 atcgtcacat gcgagatcgc gccgctgccg ggccaaaagt cattcagcgt gatcgtctcg
     2641 gtgccgttcc agctgagctt gaataggtac caagccggcg agtggttgcc gtccttgacg
     2701 agcaggtgag tggcttggct gatagccgag ccgccgttat agacgatcgt cgcgtccttg
     2761 gggtccgccg ccgtgttgta gaacgaagtc gtataagagc cggcgaagag cccgacctcg
     2821 gggccgccga cgttctgctt gtagatttcg atcgacgacc ctagcacgtc gccgatgacg
     2881 tcgttgatca cggcttgtga cgtctgattg ccggtcatca gtaggcacgt cgcgctgccg
     2941 cagatgggtg tcgatggagt gatcagcagg gcattggccg acagcggagc tagcgagaag
     3001 agcgcgagtg gcgcgagtag ggcaaagcgt ttcatggtcc tttgagcgtc cttggttcgt
     3061 aacggggccc aaatttagtt gggcgtctga tgctctagga gcaagagtcg ggccaagaac
     3121 ccaaacgatt gattttcaaa ttgtttttgt ttgagacact ggtgcgcgcc agcagaagcg
     3181 taagccgcgc cgacacttct gtcgcgcccg aatgagacgc gcgtcacgct tcgggcgccc
     3241 gttgcggagc gccgcgccag cgtggtccct atgcgtcagc gtttgaggac tgcgccttcg
     3301 tgcacgggtg cgacggtacg ctgatacacg ccgagccagc cgcgttggtc ttgggcaccg
     3361 tagctgcgcg gcgtcttgcg gcgctcgacg agcacgtctt cggagacttc gagatcaacg
     3421 gctctgcggc gcacgtcgat cgagatgacg tcgccgttct cgacataggc gagcggaccg
     3481 ccggccgccg cttcgggcga cgcttcaccg acgacgagac cgttgttgac gagccccgag
     3541 agctggcctt cggtgattgc ggcgacgcgg tcgaggagtc ccgcgccgtc gagcgcgaag
     3601 atgacggctg acgtcatcgc catgccgggg ccgccgatca cgccgaggcc gcgcagcacg
     3661 acaacgtcgc ccggtttgac gtgtccggct ttgatgccgg ccagcgcctc gtcgcgcgaa
     3721 tgaaaaatgt tcgccttgcc gcgaaactcg agcatgcgtt caccggtgcc gccgagacgc
     3781 acgatgccgc cgccgggtag caagctgccg cgcacgatga cgatcgccgg acgccgcgag
     3841 aacggtttgt ccaaggttgc gatcgtgcgc tcgtccgtga cggtcgtcgc cgcaagaatc
     3901 tcgcgcagcg tttcccccga caccgagcgc gcgtcgaggt cgagctgccg ctcgagcctc
     3961 ttcaatatgg cgcgcgcgcc gccggccgcc tcgaaatcct cgatgtgacc gtcgccgttc
     4021 ggcttgacgg ccgcgagcaa cggtactcgc tccgcgtgat cggcgaacgc ctgatagacg
     4081 tcgacgtcgg cctcggcctc ggtcgcgatc gcttgcagat gcttcacgca gttgatcgag
     4141 ccgctgacgg ccagcaccat ctgcacggcg ttgcgcacgg cacccgccgt catgatgtcg
     4201 cgcggcttca cgtcctgcca caccatttcg acgatgcgcg cgcccgcagc gcgcaaatgc
     4261 gcgtgcatct tcgcactgcc ggctgcgacg ggcgcgctgc cgggcagcgt catgccgagc
     4321 gcttcgcaga cgacgtgcat cgagttcgcg gtccccatgc cttggcagac gccgggactt
     4381 tgaatcgcga cgtcggccat gcctttgacc tcttcgatcg tcacggcgcc ggacgtcaga
     4441 taacccgtct tcaggaacac gtcctcgatg tcagtgtgtc ggccgcggta cacgcccgct
     4501 ttctgataac cgcagacgac gacgatggtc ggaatgttga gccggcccgc cgccatgagc
     4561 tgccccggtg tcgtcttgtc gcacgatgcg aggcagacca tgccgtcgag caaggcgcct
     4621 tcgacctgca cctcgatgtc ggccgcgatc aggtcgcggg tcggaaggat gtaggcgccg
     4681 cgacgccccg cgctcgtgat gaagtcgctc ggcgcagccg tgcggatctc gaagggaacg
     4741 ccgccggccg cccgaatcgc gactttgagc tcgtcagcca agccgtcgag atggctgaag
     4801 cagctcgaga gctcgctcga ggaattcacg atcgcgatct tcggcttctg catgtcgtcg
     4861 tccgtgagcc ccaaagcgcg ccattgcgcg cggcgaccgg accatggcac cgagccttct
     4921 tcgaggttgc tgcgcagctt gcgtgtcatt cgtccgctcc gagagtcgtg tcgtgcggtc
     4981 cacgataact cgggcatact ccggtcggaa gatgcgcaaa cccggccggc tcaatctaaa
     5041 aaagatcgcg gaatccgctc agccgcgccg aacacgcgcc gacgtcaaag cgttgctcga
     5101 cgccgcgccg gaagtcgtct tcgcccgctg ccgctcgccg cgctattggc cgatttggct
     5161 gtttgctttc tggctgcggc tgacggccgc tttgccgtgg cgcgtcgcca tccgcagcca
     5221 caagttgctg gggcgaatgc tcggccgcct gtcgggccgc agtcgacgcg tcgtgcgacg
     5281 caacctcgag ctctgtttcc cggagctcgg ctccgccgag attcggcgaa tcatcaaggc
     5341 gaactgcgcg aacgtcggcg cgttcttcgc cgagctcgcg ttcgcttggt acggcgaccc
     5401 cgaacggcgc tcacgactgt ttcggttcga gggcctcgag catctcgagg ctgcgatcgc
     5461 gaaaggcaaa ggagcgctgc tgtatgcagg gcatttcacg ccgatcgaga tttgcgcgcc
     5521 gatgctcaag tcgcgcattc cgttgtacgc gttcatgttc aagccgcgcc gcaatccgct
     5581 gatgaatgca ttgcagaacc gcggccgcgg ccgctacgcg cacgtgtcgg tgaccaacga
     5641 tgacgtacgc gagttgctgg ccttgctcgc tcgcaacgcc gtcatttggt attcgccgga
     5701 tcaagcgcgc gtcg
//
LOCUS       DATLEE010000473         2713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_538779, whole genome shotgun sequence.
ACCESSION   DATLEE010000473 DATLEE010000000
VERSION     DATLEE010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538779"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1417
                     /locus_tag="VM692_10225"
     CDS             137..1417
                     /locus_tag="VM692_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ52588.1"
                     /translation="MTQTSAAATPVLPWYRTITAEQWRVLAAAKVGWMLDAMDFMLYA
                     MALGSLRAYFEFDDATAGFLGTATLVMSGVGGLIFGYVADRFGRARALMATIVIFALA
                     SLGAATSQSVLQLLFWRAVLGIGMGGEWASGAVLVSETWPPQHRNKAISIMQSGWAIG
                     YIAAAVLAALILSQPSLGDEAWRWLFVVGVAPALFAIWIRRNVRESEAWVARVKPKRE
                     AAENPFKVIFGRALVGRTLAIIALGGAVQFAYWGLFFWLPPFLSRPIEQGGAGMGVVG
                     SLQWIVLMQVGAYLGYLTFGFIADKLGRRRTFILFMLCAATIVPVYGQMARNPTVLLI
                     LSPLLGYFGHGYFSLFGSLVAEQFPTAVRATGQGTSYNAGRMAGAIAPFTIGAVATLP
                     GVGIGLALGLTSAFFLAAALLVFTLRDRSGEALD"
     gene            complement(1461..1787)
                     /locus_tag="VM692_10230"
     CDS             complement(1461..1787)
                     /locus_tag="VM692_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006755200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52589.1"
                     /translation="MADSTLDPDNLPARRGRKTQKGHDVRSLGPSDSSDSGSDLAGTR
                     GRDDTSDSGGTGERITAEPDESAGLGNDIDADRVVDADDAGLGYGLDEAELAEEDSKR
                     PPEDEA"
     gene            1933..2412
                     /locus_tag="VM692_10235"
     CDS             1933..2412
                     /locus_tag="VM692_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HUQ52590.1"
                     /translation="MPLPLVLKTDAFEDGGIVPPRYGCFGDNVQPAFAFNNVPENAGS
                     LALVFHDIDVALDGTPEDGVHWTVWNIPPDAGGVPEGGLPVGAVLGRNVENRTAYLGP
                     GAPPGPRYHHYVFELFALDRELDLPATAGRAELLRAMDGHVLDKAAYVGRFRRERVN"
     gene            complement(2477..>2713)
                     /locus_tag="VM692_10240"
     CDS             complement(2477..>2713)
                     /locus_tag="VM692_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014875866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52591.1"
                     /translation="PLQHDILTGTNEDGTSSGLTCNNWTDGADATATLGHADRLGRNA
                     GVNSWNAIHPSQGCSMEKLQPTGGAGLFYCFATN"
BASE COUNT          364 a          880 c          938 g          531 t
ORIGIN      
        1 tcggagttgc cgttgacgaa agtcatccgc gcggcactac cgtggctcgt cgtgctgctc
       61 gcggcgctga tcatcgtgac gtacgtgccg tgggtgtcgc tcgtattgcc gcgagcactc
      121 gactaggctc gtgcgcatga cgcaaacctc cgccgccgca acgcctgtgc tgccgtggta
      181 ccgcacgatc accgccgagc aatggcgtgt gctcgcagcc gcgaaagtcg gctggatgct
      241 cgacgcgatg gacttcatgt tgtacgcgat ggcgctcggc tcgctgcgcg cgtacttcga
      301 gttcgacgat gcgacggcgg gttttctcgg cacggcgact cttgtgatgt cgggcgtcgg
      361 cgggctgatc ttcggctacg tcgcagatcg cttcgggcgt gcccgcgcgt tgatggcgac
      421 gatcgtcatc ttcgcattgg cctcgctcgg agcagcgacg tcgcagagcg tgctgcagct
      481 cctgttctgg cgcgccgtgc tcggcatcgg catgggcggc gaatgggcct cgggcgccgt
      541 gctcgtgagc gagacatggc cgccgcagca tcgcaacaag gcgatcagca tcatgcagtc
      601 cggctgggcg atcggctaca tcgcggccgc ggtgctcgct gcgctgatcc tgagccagcc
      661 gtcgctcggc gacgaggcgt ggcgttggct gttcgtcgtc ggcgtcgcgc cggcgctgtt
      721 cgcgatttgg attcgccgca acgtgcgcga atcggaggcc tgggtcgcgc gcgtgaaacc
      781 gaagcgcgag gcagccgaga atccgttcaa ggtcatcttc ggccgagcgc tcgtcggccg
      841 cacgctggcc atcatcgcgc tcggcggcgc cgtgcagttc gcgtactggg gcctgttctt
      901 ctggctgccg ccgttcctct cgcggccaat cgagcagggc ggcgccggca tgggcgtcgt
      961 cggctcgctg cagtggatcg tgctgatgca ggtcggcgcg tatttaggct atctcacgtt
     1021 cggcttcatc gcggacaagc tcggccgacg gcgcacgttc attctattca tgctgtgcgc
     1081 ggccacgatt gtgcccgtgt acggccagat ggcgcgcaat ccgacggtgc tgctgatttt
     1141 gagcccgctg ctcggctatt tcggccacgg ctacttcagc ctattcggca gcctcgtcgc
     1201 ggagcagttt ccgacggccg tgcgcgcgac ggggcagggt acgagctaca acgccgggcg
     1261 catggccggt gccatcgcgc cgttcacgat cggcgctgtc gcgaccttgc ccggcgtcgg
     1321 catcggcctc gcgcttgggc tgacgtcggc gttctttctc gcggccgccc tgctcgtgtt
     1381 cacgttgcgg gaccgcagcg gcgaggcgct cgactagcta ttgtcttgcg gcgcgcacac
     1441 tgcgcgagct tgtcgcttac tcacgcttcg tcttcgggcg gccgtttcga gtcctcttcc
     1501 gcgagctcgg cttcgtcgag gccgtagcct aagcccgcgt catcggcatc gacgacgcga
     1561 tcggcgtcga tgtcgttgcc gaggccggcg ctctcgtcgg gctccgcggt gatgcgctcg
     1621 ccggtgccgc cgctatcgct cgtgtcatct ctgccgcggg tgccggcgag gtccgagccg
     1681 gagtccgagc tgtcgctcgg ccccagcgag cggacgtcgt ggcctttctg ggttttgcgc
     1741 ccgcgacgcg cgggaagatt gtcgggatcg agcgtgctat ctgccatggg tcgttgctcc
     1801 taagtaatgg acgaacgggg cggcgatcgt tgcaagctcc gttccagcgc ctagcctcga
     1861 tggcgcggcc cggcacgtgt cgtggtcccg aacttgcatc gcagttaggc ccgaggctag
     1921 agaacggaac ggatgcccct gcccttagtt ctcaagacgg acgcattcga ggatggcggt
     1981 atcgttccgc cgcgttacgg ctgcttcggc gacaacgtgc aaccggcttt tgcgttcaac
     2041 aacgttcccg agaacgccgg cagcctcgca ctcgtgtttc acgacatcga tgtcgcgttg
     2101 gatggcacgc ccgaggacgg agtgcactgg acggtgtgga acattccgcc ggacgccggc
     2161 ggcgtgccgg aaggtgggtt gccggtaggc gccgttctcg gccgcaacgt ggagaaccgc
     2221 accgcgtatc tcgggcccgg cgcaccgccg gggccgcgct atcaccacta tgtgttcgag
     2281 ctgtttgcgc tcgatcggga gctcgaccta cccgcgacgg cggggcgagc cgagctgttg
     2341 cgagcgatgg acggccacgt gctcgacaag gccgcctacg tcgggcgctt tcgccgcgaa
     2401 cgcgtgaatt gaactcggtc ggtgggagag ggcgtgccgc ccgttgccga acggcacgcc
     2461 cgatgctaaa cggcgatcag ttcgtcgcga agcagtagaa caagcccgcg ccgcccgtcg
     2521 gctgcagctt ttccatcgag cagccctgcg acggatggat ggcgttccag gaattcacgc
     2581 cggcattgcg gccgagccga tccgcatggc ccagcgtcgc cgtcgcgtcc gcgccatcgg
     2641 tccagttgtt gcacgtgagg cccgacgaag tgccgtcctc gttcgtgccg gtcaggatgt
     2701 cgtgctgcag cgg
//
LOCUS       DATLEE010000474         2186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5750036, whole genome shotgun sequence.
ACCESSION   DATLEE010000474 DATLEE010000000
VERSION     DATLEE010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5750036"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1344
                     /locus_tag="VM692_10245"
     CDS             190..1344
                     /locus_tag="VM692_10245"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HUQ52592.1"
                     /translation="MRKGLRRTLLVLGIVAAVAIAARLAAPLLVERYVNRALADMGEY
                     HGSVEQINLGLIRGGYELRNLQIVKNDATAETPPFAEIPSMDLSLQWRALFKGSAVGQ
                     LVIDQPKLNLVQSEANKEKQLGTGVNWPQEIRDLFPFTLNVVEVRNGLVTFRAPGIST
                     NDSLTVRDFNMQLLNLTNVQDVAEPAFADLDVHGFIMGNAPLSLTGSIDPNEMVPTFD
                     IDLSIENAKLVDVNPWLREFLKVDAEMGTFAMYSELAAAEGRFEGYVRPILEDPKFIS
                     KNEDEGPFRKAWEGLVNLAAKILENKQEDQVATQIPLRGELADPSVDLVSTIVNLLRN
                     AFVAAFSHSLEGSINLRDVAGDVRCLGGEPPAGDQPDKEQSRRERRRDRC"
     gene            complement(1420..>2186)
                     /locus_tag="VM692_10250"
     CDS             complement(1420..>2186)
                     /locus_tag="VM692_10250"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HUQ52593.1"
                     /translation="SQFRPGPAPAVTSERYTRDYNEVKEKGGTLSHPAAGGCPAPAGT
                     DMARFWSGNFGLQWNQAARDMAVDRQLGLGDTARLLALANMAGGDAAIAVWDSKVHYY
                     VWRPITAINLGKDNGNNKTEGDPAWVPFIGGAHFPAGSQTPPYPDYVSGANGLTGAFT
                     AMLQLFFHTDNVPFEIYKAVPASVAICTNPRSFRRISDAAEEVVDARILLGIHFRFAD
                     TVARTLGSRVAWHVFANELRPLRRDWDNDDGHDHDD"
BASE COUNT          405 a          737 c          698 g          346 t
ORIGIN      
        1 acgagtaata gcggcgccat cgacaacagc gagtagtacg cgaggccggc cgcgagcggc
       61 agggcgtcgt cctcggccat ccgctggata gagtcccaaa tcacgccgcc ggccaccctc
      121 agcatgccca tcctgagcaa actctgtgcc acggcccgcc ctggcacgcg ctttgcaacc
      181 gactacggta tgcgtaaggg actacgccgc acgctcctcg tcctcggcat cgtcgccgcc
      241 gtcgccatcg cggcccgcct cgcggcaccg ctgctcgtcg agcgctacgt gaaccgggcg
      301 ctcgcggaca tgggcgagta ccacggcagt gtcgagcaaa tcaatttggg cctgattcgc
      361 ggcggctacg agctgcgcaa tctgcaaatc gtgaagaacg atgcaacggc cgagacgccg
      421 ccgttcgccg agattccgtc gatggacttg tcgctgcagt ggcgtgcgct cttcaaagga
      481 agtgccgtcg gccagctcgt catcgaccag ccgaagctca acctcgtcca gtccgaggcg
      541 aacaaggaga agcagctcgg cacgggcgtc aattggccgc aggagatccg cgatctcttt
      601 ccgttcacgt tgaacgtcgt cgaagtgcgc aacggcctcg tcacgtttcg cgcgcccggc
      661 atcagcacga acgactcgct gacggtccgc gacttcaaca tgcagctcct gaacctcacg
      721 aacgtgcagg acgtcgccga gcccgcgttc gcggatctcg acgtgcacgg cttcatcatg
      781 ggcaacgcgc cgctctcgtt gaccggcagc atcgatccga acgagatggt gccgacgttc
      841 gacatcgatc tatcgatcga gaacgcgaag ctcgtcgacg tgaacccgtg gctgcgcgag
      901 ttcctgaaag tggacgccga gatgggcacg ttcgcgatgt actcggagct cgcggccgcc
      961 gaggggcgct tcgagggcta cgtgcggccg atcctcgagg acccgaaatt catcagcaag
     1021 aacgaagacg aagggccgtt ccgcaaggca tgggaagggc tcgtgaacct cgcggccaag
     1081 atcttggaga acaaacagga agaccaggtc gcgacgcaga tcccgctacg cggcgagctc
     1141 gcggacccga gcgtcgacct cgtcagcacg atcgtgaacc tgctgcgcaa cgcattcgtc
     1201 gccgcgttca gccactcgct cgaaggctcg atcaacctgc gcgacgtcgc gggcgacgtg
     1261 cgctgcttgg gcggcgagcc gccggccggc gaccagccgg acaaagaaca atcgcggcgc
     1321 gagcgccgcc gcgaccgctg ctgagcaaac gctacataaa gagagtcggc tcgatcgagc
     1381 ggcgttgggg tgcctctcga tcgagccgaa gccgacgcat cagtcgtcgt gatcgtgacc
     1441 gtcgtcattg tcccagtcgc ggcgcagcgg ccgcagctcg ttcgcgaaca cgtgccacgc
     1501 gacccgcgag ccgagcgtgc gtgcgaccgt atccgcgaag cggaagtgga tgccgagcag
     1561 aatacgcgcg tcgacgactt cctcggccgc atcggagatg cgccgaaagc tccgcgggtt
     1621 cgtgcaaatc gccacggacg ccggcacagc cttgtagatc tcgaacggca cgttgtccgt
     1681 gtggaaaaac agctgcagca tcgccgtgaa cgctccggtc agcccgttcg cgcccgacac
     1741 gtagtcggga tacggcggcg tctgcgagcc ggccgggaag tgcgcgccgc cgatgaacgg
     1801 tacccacgcc ggatcgcctt ccgtcttgtt attgccattg tccttgccga ggttgatcgc
     1861 ggtgatcggg cgccacacat agtagtggac cttgctgtcc cagacggcga tcgccgcgtc
     1921 gccgcccgcc atattcgcga gcgccagcaa gcgcgccgtg tcgccgagac cgagctgccg
     1981 gtcgaccgcc atgtcgcgcg cagcctggtt ccactgtagg ccgaagttgc cggaccagaa
     2041 gcgagccatg tcggtgccgg ccggtgccgg gcaaccgcca gccgccggat gcgacaacgt
     2101 gccgcccttc tccttcactt cgttgtagtc gcgcgtgtaa cgttcgctcg tcaccgccgg
     2161 cgccgggccc ggacgaaact gcgacg
//
LOCUS       DATLEE010000475         4329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4239463, whole genome shotgun sequence.
ACCESSION   DATLEE010000475 DATLEE010000000
VERSION     DATLEE010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4239463"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VM692_10255"
     CDS             complement(<1..386)
                     /locus_tag="VM692_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUQ52594.1"
                     /translation="MNTSVASPKGRSMPAGPLLALALLALLWGYNWVVMKVAMQYVGP
                     LDFAAMRGVFGTVLLFAVLLSVGAPLKPQHVGKTILLGLFQTAGFVGLISWALKSGSA
                     GKSAVLGYTMPFWVIVFGWPFLPDRLR"
     gene            620..1444
                     /locus_tag="VM692_10260"
     CDS             620..1444
                     /locus_tag="VM692_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ52595.1"
                     /translation="MGVALKGKTALVTGSTDGLGKEVARQLGALGARVLVHGRNQERG
                     DEVVRVIRAAGGEATFYRADLASLASVDSLASEIRERHDRLELLINNAAAVSGPERQT
                     SADGHELAFAVNYLAHFMLTYRLLPLLEAGAPARIVNVSSLGQTPLDFSDLMMERSYD
                     PGDAYRRSKLAQIMFTIDLAAELDAAKVTVNSLHPARSMNTARVINGGFQPLSTVEEG
                     AEATMQLAVSPELAGRTGLYFNGLNEARANAQAYDEAARAQLRALSLELAGLTGRS"
     gene            complement(1449..2006)
                     /locus_tag="VM692_10265"
     CDS             complement(1449..2006)
                     /locus_tag="VM692_10265"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HUQ52596.1"
                     /translation="MERASRIAGISVLVALLVAGPTAAQPPAERTPPLSPLIADSLAP
                     LLAMLITAERDNAKAQGVEPIPAAIRAALQGYVPEETLDRVRWREGGTVLSLPQNVIR
                     FGHVPAMTLDDVIVFQERRAALEDPKLWAHELTHVVQFARWGVSGFATQYLRDYEAVE
                     REAAEFRWQFMKEKGLVPAVAPPRE"
     gene            complement(2020..2412)
                     /locus_tag="VM692_10270"
     CDS             complement(2020..2412)
                     /locus_tag="VM692_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HUQ52597.1"
                     /translation="MARKNFSSGSKWEPIIGYSRAVRVGAYVHVSGTTAGGADGQIVG
                     VGDLAAQTKQCLRNIESALEKAGAKLSDVVRTRMFLVNIGEWELAGKAHGEYFSSIRP
                     VTTMVEVSKLISPEMLIEIEADAYVGSD"
     gene            complement(2430..3377)
                     /locus_tag="VM692_10275"
     CDS             complement(2430..3377)
                     /locus_tag="VM692_10275"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HUQ52598.1"
                     /translation="MKQLVLWLALLVCPMALAQDRIIVSGASGQLGSLVIDELLARKV
                     SPENLILVSRTPNSDHLQAYAARGASVRFGDFNEPESLDAAYRGGTRMLLISINGGGG
                     NRRELHKGASDAAARVGVKQIAYTSYVNADRYEDSTIAVDHRRTELILEESGVAWTML
                     RNQIYANGLLDQAVQIVRDGRYVTHTPDARVAYVTREDCAAAAAAVLATPGHENKAYN
                     ITGPDAIGPRELVALASEISGKPVELVVLSEADYRAQLTASGVPEAAINGTISFAAEL
                     ESQYLQEPSTAVADLTGRPATSVRALLLQNRERLRDAAN"
     gene            complement(3374..4132)
                     /locus_tag="VM692_10280"
     CDS             complement(3374..4132)
                     /locus_tag="VM692_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HUQ52599.1"
                     /translation="MPERIHKVLANAGIGSRRSIERWVAEGRITVDGRVAKLGEQLQG
                     RERVCFDGKPIRLTAAKLARPSSDFLAYYKPGATRGTGEDKAALELPRPRQGRWIDVS
                     PLDRNTSGLSVLTTDGELASRLMHPSTVIEREYAVRLLGTPSDAQLRQLEQGIELDTG
                     LAKVETVEARRGTGSNAWHHLTLHEARHRDLRPMLAAVGLAVSRVIRVRYDVIALGEL
                     YRGASRPLAPAETKSLYALAGLAQGRREQRKDQA"
BASE COUNT          733 a         1471 c         1387 g          738 t
ORIGIN      
        1 cgcaagcggt ccggcaagaa cggccagccg aacacgatga cccagaacgg catcgtgtag
       61 ccgagcacgg cgctcttccc cgcgctgccc gatttcaagg cccaggaaat caggccgacg
      121 aatcccgccg tttggaacaa gccgagcaag atcgtcttgc cgacgtgctg cggtttcaat
      181 ggcgcgccga cactcaagag gaccgcgaac aacaacaccg tgccgaagac tccgcgcatc
      241 gcggcgaaat cgagcgggcc gacgtactgc atcgcgacct tcatcacgac ccagttgtag
      301 ccccagagca aagccagcag cgcgagcgcc agcaaggggc ccgccggcat gctgcgacct
      361 ttcggcgaag cgacagatgt gttcatcgat gcggctactt tacctgcttc gcgctcgttc
      421 gcgtcggttg ccagccgact gcgccgccga ctatcgttaa gtcttcacgg agaacgagac
      481 cattcggatg aacatgatgc attcgcgccg ctcgactttg ctcgcgggcg tcgggctcgc
      541 agtgggcgcg gcgctgccgg cgaccgcgca accgcaagcc gagcggcgca caccgcagag
      601 cgaagcgcgt tgggcacgca tgggcgtcgc attgaagggt aagacggcgc tcgtgacggg
      661 ttcgacggat ggcctcggca aggaagtcgc gagacagctc ggcgcgctgg gcgcgcgcgt
      721 cctcgtgcac ggccgcaacc aggagcgagg cgacgaagtc gtccgcgtca tccgcgcggc
      781 cggcggcgag gcgacgttct accgcgcgga cctcgcgtcg ctcgccagcg tcgactcgtt
      841 ggcgtcggag attcgcgagc ggcacgaccg gctcgagttg ctgatcaaca acgcggcggc
      901 agtcagcggg cccgagcgcc agacgagcgc cgacggtcac gagctcgcgt tcgccgtgaa
      961 ctatctcgcc cacttcatgc ttacctatcg cctcttgccg ctcctcgagg caggcgcccc
     1021 cgcgcgcatc gtcaacgtct cgtcgctcgg ccaaacgccg ctcgacttca gcgacctcat
     1081 gatggagcgc agctacgacc ccggcgacgc ttatcgccgc agcaagctcg cacagatcat
     1141 gttcacgatc gacctcgctg ccgagctcga tgccgcgaag gtcacggtca atagcttgca
     1201 tcccgcccgc tcgatgaaca cggcgcgcgt catcaacggg ggatttcagc cgttgagcac
     1261 cgtcgaagaa ggcgccgaag cgacgatgca gctcgcggtc tcgccggagc tcgcgggtcg
     1321 cacgggactc tacttcaacg ggctcaacga ggcgcgtgcg aacgctcaag cctatgacga
     1381 agcggcgcgt gcgcagctgc gtgcattgag cctcgagctc gcggggctga cgggaagaag
     1441 ctgaccgatt actcgcgcgg aggggccacc gcgggaacga ggcctttctc cttcatgaac
     1501 tgccaacgaa actccgcggc ttcgcgctcg acggcttcat agtcacgcag atactgcgtc
     1561 gcaaatccgc tcacgcccca tctcgcgaat tgcacgacgt gcgtaagctc gtgcgcccag
     1621 agcttgggat cttcgagagc cgcgcgccgc tcctgaaaaa cgatgacgtc gtcgagcgtc
     1681 atggccggca cgtgcccgaa gcggatcacg ttttgcggca gcgacagcac cgtaccacct
     1741 tctcgccagc gcactcggtc gagcgtctct tcgggcacat agccctgcag cgccgcgcgg
     1801 atcgcggcag ggatcggctc gacgccttgc gccttggcgt tgtcgcgctc ggccgtgatc
     1861 aacattgcca gcagcggggc taaagaatcg gcgatcagcg ggctcaacgg cggtgtccgc
     1921 tcggcgggcg gctgcgcggc cgtcggcccg gccacgagga gagcgaccaa gacagaaata
     1981 ccggcaatac gggaagctcg ctccatgagc tcggttgcct cagtccgagc cgacgtaagc
     2041 gtcggcttcg atctcgatca gcatctcggg tgagatgagc ttcgacactt cgaccatcgt
     2101 cgtcacaggg cggatcgagc tgaagtactc gccatgtgct ttgccggcca gctcccactc
     2161 gccaatgttg acgaggaaca tgcgtgtccg gacgacgtcg ctgagcttgg ctccggcttt
     2221 ctcgagcgcg ctctcgatgt tgcgcaagca ctgcttcgtc tgggcggcca aatcgccgac
     2281 gccgacgatc tgcccgtcgg caccgcccgc cgtcgtgcct gaaacgtgga catacgcccc
     2341 gacgcgcacg gcgcgcgagt agccgatgat tggttcccac ttcgagcccg acgaaaaatt
     2401 tttccttgcc atccgtttgc tccccgtgac taattggccg cgtcgcgcag ccgctcgcga
     2461 ttctgcagca ataatgcgcg gacgctcgtc gccggccgcc ccgtcaggtc cgctacggca
     2521 gtgctgggct cttgcaagta ttgtgactcg agctcggcgg cgaacgaaat cgtgccgtta
     2581 attgccgcct ccggcacgcc ggaagcggtg agctgtgccc ggtaatccgc ttcgctgagc
     2641 accacgagct cgacaggctt gccgctgatc tcgctggcca atgcgacgag ctcgcgagga
     2701 ccgatggcgt cgggtccggt gatgttgtac gccttgttct cgtgccccgg tgtcgcgagc
     2761 acggccgctg ccgccgccgc gcaatcctca cgcgtgacat aggcgacgcg cgcgtcgggc
     2821 gtatgcgtca cgtagcgtcc gtcgcgaacg atctgcacgg cctggtcgag gagaccgttc
     2881 gcgtagattt ggttgcgcag catcgtccac gccaccccgc tctcctcgag gatgagctcc
     2941 gtgcggcggt ggtcgacggc gatcgtcgag tcctcgtacc gatcggcgtt gacgtacgac
     3001 gtataagcga tctgcttgac cccgacgcga gcggccgcgt cgctggcgcc cttgtgcagc
     3061 tcgcgccgat tgccaccgcc gccgttgatg ctgatcaaaa gcatgcgcgt gccgccgcga
     3121 tacgccgcat cgagcgactc cggctcgttg aagtcgccga accgcacgct cgcgccgcgc
     3181 gccgcgtaag cctgcaagtg atcgctgttc ggagttcgac tgacgagaat cagattctcc
     3241 ggcgacactt tgcgcgcgag cagctcatcg atgacgaggc taccgagctg ccccgaggct
     3301 cccgaaacga tgatcctgtc ttgggcgagc gccatcggac acacgagcag cgccagccat
     3361 agcacgagct gtttcatgct tgatccttgc gctgctcgcg gcggccctgc gccaaacccg
     3421 caagcgcgta cagcgatttc gtctcggccg gggcaagcgg ccggctcgcg ccgcgataga
     3481 gctcgccgag cgcaatcaca tcgtagcgca cccggatcac gcggctgacc gcgagcccga
     3541 cggcggccag catcgggcgc agatcgcggt ggcgagcctc gtgcagcgtc aggtgatgcc
     3601 acgcgttgct accggtgccg cggcgcgcct cgactgtttc cactttggct agaccggtgt
     3661 cgagctcgat gccttgctcg agttgccgca gttgtgcgtc cgagggagtg ccaagcaaac
     3721 gcacggcgta ctcgcgctcg atgaccgtgc tcgggtgcat cagacggctc gcgagctcac
     3781 cgtccgtggt cagaaccgac agccctgagg tgttgcggtc gagagggctc acgtcgatcc
     3841 agcggccctg gcgcggccga ggtagctcga gcgcggcctt gtcctcgccg gttccgcgcg
     3901 tcgctcccgg cttgtagtac gccagaaagt ccgagctcgg ccgcgcgagc ttggcggcgg
     3961 tcaaccggat cggcttgccg tcaaagcaga cgcgttcgcg accttgcaac tgctcgccga
     4021 gcttcgcgac tctaccgtcg acggtaatcc gaccctcggc cacccagcgc tcgatcgagc
     4081 gtcgcgagcc gatgccggca ttggccagaa ccttgtggat tcgctcgggc acggctcacc
     4141 gtcaactcga gctactaatt gtcgtcgtcg gcggcgcgca gcggaatcac gtccgcggat
     4201 tgcacggctg cgtcttcggc ttcctcggcg gcaccgtcga ccgcacccgg cgtgtcgtcg
     4261 tcggcctcct cgccgatcag gtcggggccg gtcagatcgg actcgtcgtc ggcggagtca
     4321 tcgtcgtcg
//
LOCUS       DATLEE010000476         6856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8466681, whole genome shotgun sequence.
ACCESSION   DATLEE010000476 DATLEE010000000
VERSION     DATLEE010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8466681"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VM692_10285"
     CDS             complement(<1..1323)
                     /locus_tag="VM692_10285"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HUQ52600.1"
                     /translation="MTPSRLILTGAGVLAAAAVGTAVVVHTDLLGPSAEEQWTLVQTY
                     CVDCHNAAEAAGGVVFEGKGPDAVAHDPQLFEAAVRKLRGRLMPPPGNPQPEQPRVDS
                     LVAWLERTIDRDAAMPHAGHVPIQRLNRTEYAAAVRDLVGVEIDAAEFLPAEIEVDGL
                     DKIAAALSISPAFLDQYIAAARHVAHLAVGETVPKVTSVSFPAPPNEEDQDEYVDGMP
                     LGTRGGMRFTHNFLADGEYRITIADLDVGQYARSLESENTVVMLLDRNEVFRSKLGGR
                     DELMFYNRDGAPAKAEIMKRFTKIPVQITAGVHELAVTFIERSRAATEDHIFGFQPYG
                     GFSYTGKMRVPKIIGTGVQVEGPFGVTSVSRTPSRDKIFVCTPGATADERACAERIAA
                     SLATRAFRRPAGADDVERLLPFYDAGRAGGSFDTGVEQLVTAVLASPDF"
     gene            1476..1868
                     /locus_tag="VM692_10290"
     CDS             1476..1868
                     /locus_tag="VM692_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52601.1"
                     /translation="MQRQIRTALAAALVFGSSGALAQDGTLAGVTMRVLDDLQDVDAV
                     VLELDAGRGETEDGAPSPEPREGAAAEGAGRSRDTAAREVAAEGERRAERRERDALHD
                     VDEDERAEGRLEDRDVERSTQPPPPAAP"
     gene            complement(1865..2599)
                     /locus_tag="VM692_10295"
     CDS             complement(1865..2599)
                     /locus_tag="VM692_10295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HUQ52602.1"
                     /translation="MLKASLKRFGLPAAVIFVTAGSIVLSVAISATIHALIGMPMRGS
                     AWAVTVLCPAIIGPVMSWWTFDLVLKVERAHEQLRLQSNIDHLTGIFNRRYFMDRLRE
                     EIDRCQRHGTSFAVAFIDVDNFKRINDEHGHLSGDEILCQLTQICTKQVRDIDTLARI
                     GGEEFALLLPQTTAAEAAQLVERLRASVAATRAKVGDGWLDVTVSIGLTGSSRDRLDV
                     NGILRRADEALYAAKRQGKNRLAALA"
     gene            complement(2715..3629)
                     /locus_tag="VM692_10300"
     CDS             complement(2715..3629)
                     /locus_tag="VM692_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HUQ52603.1"
                     /translation="MTMNRRSFLTRSSAAAFVSLVGFEALAQRGCAGPPAGVQLYTVR
                     DALGRDPRAALTQLREIGIVEAELYGLNGAANATLFGMPASDLKKACEANGIRLPATH
                     IGGDLTNHAEIAEIARALGVEAVIVALPSEFSGQVDGRFAMVPAKSRAQLDTLAEKLN
                     RAGREYRDRGLTFGYHNHHIEFMPVDNVVPYDYLLSNTDPGLVRIELDIGWLATAGAD
                     PVAYLRKHAGRVLSCHLKDYNPTIVSDVPQRKVVAPGAGRVDFGAVLAAMRETGVAHG
                     YIEVDVSDDPFGDVRRGHAHLRKLQGCA"
     gene            complement(3646..4488)
                     /locus_tag="VM692_10305"
     CDS             complement(3646..4488)
                     /locus_tag="VM692_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009048224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HUQ52604.1"
                     /translation="MASRRFNTVDVFTAKPLYGNALAVVHDADDLSDAAMQKFANWTN
                     LSETTFLLPPTDSQADYRVRIFTPDRELPFAGHPTLGSCHAWLAAGGKPKRRDEIVQQ
                     CGVGLVRVRPDGERLAFAAPPLIRTGAPDAATLASIARSLRIASGSIRAAQWISNGPG
                     WVGVLVGSRDELLALKPDFAAMEPRDIGVVTPLPPKHEAQFEVRAFIGGVGVGPNEDP
                     VTGSLQAGLAQWLIGAGLAPDRYVAIQGTAVGRDGRVYVEKIGKDIWIGGDCVTLVEG
                     EVEL"
     gene            complement(4481..5725)
                     /locus_tag="VM692_10310"
     CDS             complement(4481..5725)
                     /locus_tag="VM692_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HUQ52605.1"
                     /translation="MSTGIYTRLSVMMFLEFFIWGAWFVTMGSYLTGGLQFSDPESAL
                     AYSTQSWGAIIAPFIIGLIADKYVNAERLLGVIHLIGAVLMYLLANATEFSAFYPYLL
                     VYMILFMPTLALVNAISFRQLDDPSRRFGLIRVWGTLGWIIAGLLISYAFDWDSQTAL
                     LRNTFTMAAAASAVLGIFSFTLPATPPQKPGGGGLREALGLDALALLRDRNFLTFFVA
                     SILVCIPLAFYYQHANQFLTEIQVANAAGIQTLGQVSELGFMVLLPFFLKRFGMKLTL
                     LVGMLAWAARYVLFAYGDPGELVFMLIAGIALHGVCYDFFFVSGQVFTDSKAGLRYKS
                     AAQGLITLATYGVGMLIGFWVAGQLTSAFAVDAGGHDWRSIWLYPAAFAGLVAVLFAL
                     LFRNEVVAYKPAPTGTPARDHG"
     gene            complement(5739..6788)
                     /locus_tag="VM692_10315"
     CDS             complement(5739..6788)
                     /locus_tag="VM692_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HUQ52606.1"
                     /translation="MKGPAIFLAQFAGDKPPFDKLESMAQWAARLGYVGVQVPTDPKL
                     FDLEQAANSQTYCDELAGELADADIVISELSTHLQGQLVAVHPAYAPQFAAFAPAHLR
                     DKPSAWSAWATDQLKHAAKASERLKLKAHATFSGALLWHTMYPWPQRPAGLVEAGFKE
                     LARRWRPILDAFDAAGVDVAFEIHPGEDLHDGATFERFLDAVGGHERANILYDPSHFL
                     LQQLDYAAFIDLYHERIKAFHVKDAEFRPNGRSGVYGGYQGWVDRPGRFRSPGDGQID
                     FKRIFSKFAQYGYDGWAVLEWECALKHPEDGAREGAPFIRSQMIRKTPQAFDDFAAGS
                     ADAKTHARILGLDQQ"
BASE COUNT         1149 a         2270 c         2364 g         1073 t
ORIGIN      
        1 gaagtcgggg ctcgcgagca cggccgtcac gagctgctcg acgcccgtat cgaagctacc
       61 gccggcacgg cccgcgtcgt aaaacggcag caagcgctcg acgtcgtcag cgccagccgg
      121 gcggcggaag gcgcgcgtgg cgagcgaggc tgcgatgcgc tctgcgcacg cgcgctcgtc
      181 ggccgtggcc cccggggtgc agacgaagat cttgtcgcgg ctcggcgtgc gcgacacgct
      241 cgtcacgccg aacggccctt cgacctgaac gcccgtgccg atgattttcg gcacgcgcat
      301 cttgcccgtg tagctgaaac cgccgtaggg ctggaagccg aagatgtggt cctcggtcgc
      361 ggcgcgcgag cgctcgatga acgtgacggc gagctcgtgc acgccggccg tgatctgcac
      421 gggaatcttc gtgaaccgct tcatgatctc ggctttcgcc ggcgcgccgt cgcggttgta
      481 gaacatgagc tcgtcgcggc cgccgagctt gctgcggaac acctcgttgc ggtcgagcag
      541 catgacgacc gtgttctcgc tctcgagcga acgcgcgtat tgcccgacgt cgaggtccgc
      601 gatcgtgatg cggtactcgc cgtcggcgag gaaattgtgc gtgaagcgca tgccgccgcg
      661 cgtgccgagc ggcatgccgt cgacgtactc gtcctggtct tcttcgttcg gcggcgccgg
      721 gaacgacacg ctcgtgacct tcggcaccgt ctcgccgacg gcgaggtgcg cgacatgacg
      781 cgcggccgcg atgtactgat cgaggaacgc cggcgagatg ctgagcgctg ccgcaatctt
      841 gtcgaggccg tcgacctcga tctcggccgg caggaattcg gccgcatcga tctcgacgcc
      901 gacgaggtcg cggaccgccg ctgcgtactc ggtgcggttc aagcgctgga tcggcacgtg
      961 gcccgcgtgc ggcatcgcgg cgtcacggtc gatcgtccgc tcgagccagg cgaccagcga
     1021 gtcgacgcgc ggctgctcgg gctgcgggtt gccaggcggc ggcatcaggc ggccgcgcag
     1081 cttgcgaacc gccgcttcga acaactgggg gtcgtgcgct accgcatcgg ggcctttacc
     1141 ctcgaacacg acgccgcccg cggcttcggc ggcgttgtga caatcgacgc aataggtctg
     1201 aaccagcgtc cactgctcct cggcgctcgg gccgagcaga tcagtgtgca caacgacggc
     1261 cgtgccgacg gcggccgcgg ccagcacgcc ggcgccggtc agaatgagac gggaaggggt
     1321 cataagagac tgtttataaa ggagtgcctt agggccgtcc agccgccgtg gctaatctag
     1381 agatagcggc cgagtgtgct ccgcatcaac gtcggtcccg agcggagctg tacgatggca
     1441 gcacgtctca tagaagcgtt cttggagtca gcctcatgca acggcaaata cggacggcac
     1501 tcgcggcggc gctggtattc ggctcgagcg gcgcgctggc gcaagacggc acccttgccg
     1561 gcgtgacgat gcgcgtgctc gacgacttgc aagatgtcga tgccgtcgtg ctcgagctcg
     1621 acgccggccg cggcgaaacc gaggacggcg caccgagccc ggagccgcgc gaaggcgcgg
     1681 cagccgaagg cgcagggcgc agccgtgaca cggccgctcg cgaagtcgcg gccgaaggcg
     1741 agcggcgcgc cgagcgccgc gagcgcgatg cgctgcacga cgtcgacgaa gacgagcgcg
     1801 ccgaaggccg gctagaggat cgcgacgtcg aacgatcgac gcagccgccg ccgcccgcgg
     1861 cgccctaagc gagcgcggcg agccggttct ttccctgccg cttggcggcg tagagcgcct
     1921 cgtcggcgcg ccgcaagatt ccgttgacgt cgagcctgtc gcggctcgag cccgtcaggc
     1981 cgatgctcac ggtgacgtcg agccagccgt cgccgacctt ggcgcgcgtg gccgcgacgc
     2041 tcgcgcgcaa gcgctcgacg agttgcgcgg cctccgcggc cgtcgtctgc ggcaacagca
     2101 gcgcgaactc ttcgccgccg atgcgtgcca gcgtgtcgat gtcgcgcact tgcttggtgc
     2161 agatttgcgt gagctggcag aggatctcgt cgccgctcaa gtggccgtgc tcgtcgttga
     2221 tgcgtttgaa attgtcgacg tcgatgaatg cgaccgcgaa cgacgtgccg tgccgctggc
     2281 aacggtcgat ctcctcgcgc agccggtcca tgaagtagcg gcggttgaag atgccggtca
     2341 ggtgatcgat gttgctctgc agccgcagct gctcgtgcgc gcgctcgacc ttcagcacga
     2401 gatcgaacgt ccaccacgac atgacgggtc cgatgatcgc agggcacaag acggtgaccg
     2461 cccaggcaga gccgcgcatc ggcatgccga tcagcgcgtg gatcgttgcc gaaatagcga
     2521 ccgacagcac gatcgagccg gccgtgacga aaatgacggc ggccggcaga ccgaagcgtt
     2581 tcaatgacgc cttgagcatt gcgttgtgtc cgcggtccct gcgagtcggt tcagctgcga
     2641 gcgatcggaa ccccgcgccg actctacgct cagcgcggcg aaaacgcgac gacgcagctg
     2701 ttgagtgctg tcggctacgc gcagccctga agcttccgca gatgcgcgtg gccgcggcgc
     2761 acgtcgccga acggatcgtc ggagacgtcg acctcgatgt agccgtgcgc aacgcccgtc
     2821 tcgcgcatgg cggcgagcac ggcgccgaag tcgacgcggc cggcgccggg cgcgacgacc
     2881 ttgcgctgcg gcacgtcgct gacgatcgtg gggttgtagt ccttcaagtg gcacgacagc
     2941 acgcggcctg cgtgcttgcg tagataggca acgggatcgg cgcccgcggt cgcgagccag
     3001 ccgatgtcga gctcgattct cacgagcccg ggatccgtgt tgctcagcag gtagtcgtac
     3061 ggcacgacgt tgtcgacggg catgaattcg atgtgatggt tgtgatagcc gaacgtgagg
     3121 ccgcggtcgc gatactcgcg gccggcgcgg ttcaatttct cggcgagcgt gtcgagctgc
     3181 gcgcggctct tggccggcac catcgcaaac cggccgtcga cttggcccga gaactcgctc
     3241 ggcaacgcga cgatgacggc ctcgacgccg agcgcgcggg cgatctccgc gatctcggcg
     3301 tggttcgtca agtcgccgcc gatgtgcgtt gcgggcaagc gtatgccgtt cgcctcgcat
     3361 gccttcttta gatcgctcgc cggcatgccg aacagcgtcg cgttggccgc gccgttcaag
     3421 ccgtagagct cggcctcgac gatgccgatc tcgcgcaatt gcgtgagcgc ggcgcgcggg
     3481 tcgcggccga gcgcgtcgcg caccgtgtag agctgcacgc cggccggtgg accggcgcag
     3541 ccgcgctgtg cgagcgcttc gaagccaacg agcgagacaa aggcggccgc gctgctgcgc
     3601 gtcaggaaac tccggcggtt catcgtcatg gtgcgctccg cgtcctcata gctcgacttc
     3661 gccttcgacg agcgtcacgc agtcgccgcc gatccagatg tccttgccga tcttctcgac
     3721 gtagacgcgc ccgtcgcgcc cgacggccgt gccttgaatc gcgacgtagc ggtccggcgc
     3781 gagcccggcg ccgatcagcc attgcgcgag gcccgcctgc aggctgcccg tgacggggtc
     3841 ctcgttcggc ccgacgccga cgccgccgat gaaggcgcgc acctcgaatt gcgcttcgtg
     3901 cttcggcggc agcggcgtca cgacgccgat gtcgcgcggt tccatcgccg cgaagtcggg
     3961 cttcagcgcg agcagctcgt cgcgcgagcc gacgagcacg ccgacccaac ccgggccgtt
     4021 gctgatccac tgcgccgcgc gaatcgagcc cgacgcgatg cgcagactgc gcgcgatcga
     4081 cgcgagcgtc gcggcatcgg gcgcgccggt gcggatcagc ggcggcgccg cgaacgcgag
     4141 ccgctcgccg tcgggccgca cgcgcacgag gccgacgccg cattgctgca cgatctcgtc
     4201 gcgcctcttc ggcttgccgc cggccgcgag ccacgcgtgg caggagccga gcgtcgggtg
     4261 gcccgcgaac ggcagctcgc ggtccggcgt gaagattcgg acgcgatagt cggcttgcga
     4321 atcggtcggc ggcagcagga acgtcgtctc gctcaagttc gtccagttcg cgaacttctg
     4381 catcgcggcg tcgctcaagt catcggcgtc gtggacgacc gcgagcgcat tgccgtagag
     4441 cggcttcgcc gtaaagacgt cgaccgtgtt gaaacgacgg ctagccatgg tcgcgtgccg
     4501 gtgtgccggt cggggctggc ttgtacgcga cgacttcgtt gcggaacagc agcgcaaaca
     4561 acacggcgac caggccggca aacgcggccg gatagagcca gatcgagcgc caatcgtggc
     4621 cgccggcgtc gacggcgaag gcgctcgtca gttgtcccgc gacccaaaag ccgatcagca
     4681 tgccgacgcc gtacgtcgcg agcgtgatca ggccctgcgc ggcgctcttg taacgcagcc
     4741 cggccttcga atccgtgaag acctggcccg agacgaagaa gaagtcgtag cagacgccgt
     4801 gcaacgcgat gcccgcgatc aacatgaaca cgagctcgcc gggatcgccg tacgcaaaca
     4861 gcacgtaacg cgcggcccaa gccagcatgc cgaccaatag cgtcagcttc atgccgaagc
     4921 gcttcaagaa gaacggcagc agcaccatga agccgagctc ggacacctgg ccgagcgtct
     4981 ggatgccggc ggcgttcgca acttggattt cggtcaggaa ctgattcgcg tgctggtagt
     5041 agaacgcgag cgggatgcag accaggatcg aagcgacgaa gaaggtcagg aagttgcggt
     5101 cgcgcaagag cgcgagcgcg tcgagcccca acgcttcgcg caagccgccg ccgccgggtt
     5161 tctgcggcgg cgtggccggc agcgtgaagc tgaagatgcc gagcacggcc gacgcggcgg
     5221 ctgccatcgt gaacgtgttg cgcagcaacg ccgtctgcga gtcccaatcg aacgcgtagc
     5281 tgatcaatag gcccgcgatg atccagccga gcgtgcccca aacgcggatc aggccgaagc
     5341 gccgcgacgg gtcgtcgagc tgccgaaacg agatcgcgtt gacgagcgcc agcgtcggca
     5401 tgaacaagat catgtagacg agcagatacg gatagaacgc cgaaaactcc gtcgcgttcg
     5461 caagcaagta catcagcacc gcgccgatca ggtggatgac gccgagcagc cgctcggcat
     5521 tcacgtactt gtcggcgatg agcccgatga tgaacggcgc gatgatcgcg ccccacgact
     5581 gcgtgctgta cgcgagcgcg ctttccgggt cgctgaactg cagcccgccc gtcaggtagc
     5641 tgcccatcgt cacgaaccac gcgccccaaa tgaagaactc gagaaacatc atgacgctca
     5701 agcgcgtgta gattccggtg ctcaacgttc gctcctcgct attgttggtc caggccgaga
     5761 atgcgcgcgt gcgttttcgc atcggccgag ccggcggcga aatcgtcgaa cgcttgcggc
     5821 gtcttgcgaa tcatctgtga gcgaatgaac ggcgcgcctt cgcgcgcgcc gtcctcggga
     5881 tgcttcaacg cgcattccca ctcgagcacg gcccagccgt cgtaaccgta ctgcgcgaat
     5941 ttgctgaaga tgcgtttgaa gtcgatctgg ccgtcgcccg gcgagcggaa ccggcccggc
     6001 cggtcgaccc agccctggta gccgccgtac acccccgagc ggccattggg ccgaaactcg
     6061 gcgtccttta cgtgaaacgc cttgatgcgc tcgtgataga gatcgatgaa cgccgcgtag
     6121 tcgagctgct gcagcaggaa gtggctcggg tcgtagagga tgttcgcgcg ctcgtgaccg
     6181 ccgacggcat caaggaagcg ttcgaacgtc gcgccgtcgt gcagatcctc cccgggatgg
     6241 atctcgaacg cgacgtcgac gccggccgcg tcgaacgcgt cgaggatggg ccgccagcgt
     6301 cgcgcgagct ccttgaagcc cgcctcgacg agcccggcgg gccgctgcgg ccacggatac
     6361 atcgtgtgcc acagcagcgc gccgctgaac gttgcgtgcg ctttgagctt aagccgctcg
     6421 ctggccttgg ccgcgtgctt cagttggtcc gtcgcccacg cgctccaagc gctcggcttg
     6481 tcgcgcagat gggcgggcgc gaatgccgcg aactgcggcg cgtaggccgg gtgcacggcg
     6541 acgagctggc cctgcaagtg cgtcgagagc tcgctgatga cgatgtcggc gtcggcgagc
     6601 tcgccggcga gctcgtcgca gtacgtctgg ctgtttgccg cttgctcgag gtcgaacagc
     6661 ttggggtccg tcggcacctg cacgccgacg tagccgagac gggcggccca ttgcgccatc
     6721 gactcgagct tgtcgaacgg cggcttgtcg cccgcaaact gcgccagaaa aatcgcgggg
     6781 cctttcatcg cagacggacc tcgagcgcgc gtagccaagg agcacgccga gtatacccgg
     6841 ccggtacgcg gcaccc
//
LOCUS       DATLEE010000477         2216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_542718, whole genome shotgun sequence.
ACCESSION   DATLEE010000477 DATLEE010000000
VERSION     DATLEE010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542718"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VM692_10320"
     CDS             <1..245
                     /locus_tag="VM692_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52607.1"
                     /translation="DVHAGFRRELPDRVALLHRLTPYHGTAGIVPEAHPEKFLTSQMD
                     KWEFFQDAAKLWRWRCTSADGHRVFDSALRLDNDAP"
     gene            258..497
                     /locus_tag="VM692_10325"
     CDS             258..497
                     /locus_tag="VM692_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52608.1"
                     /translation="MAKVTEHELTALKAKYLAAHSDYDRCVATLSQAGLGSAALLPEE
                     ILSRLAKAFADLQLARHEYRAALFDVAFAADEALS"
     gene            604..1068
                     /locus_tag="VM692_10330"
     CDS             604..1068
                     /locus_tag="VM692_10330"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52609.1"
                     /translation="MKPGYVVLLAASTLLMALPSAAHHSVAINFERGEPIEIRGTVKE
                     VHMRNPHSQYVVSVPAADGTATDWFVEWSDRNSLVRRGVDLELIKPGDTVTLTLWPSQ
                     RLPNVGYFVQAVLADGSVYRDCGFVQYREAVVKQSKFRCEEGTRSAPQREAQ"
     gene            1065..1745
                     /locus_tag="VM692_10335"
     CDS             1065..1745
                     /locus_tag="VM692_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52610.1"
                     /translation="MKIGAATAAALLAVIATAASAAGTRSDLDGVWLFYASQVPDDPG
                     LTPAGRAKLEQYDPLRDDADTDCKPVTFTNIMHTPSPPIEIRLHEADVEINYEFMDVH
                     RRVPLNGTLATAPLTVARHQHMGRSLGRYEGDELVVETVDVEAGYLDTLGFAGLPQSA
                     QMRTEERFIPDGDRLKVVVTHHDPVYYTSPMVMTYDFVRVDTKIMEWGCTLEGANYLE
                     RLKDAEAK"
     gene            complement(1755..>2216)
                     /locus_tag="VM692_10340"
     CDS             complement(1755..>2216)
                     /locus_tag="VM692_10340"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HUQ52611.1"
                     /translation="VMAKPTRTNTAAVFNLLDTDLVCVLAQLRMAACLGTPLRHFEAA
                     TVLHYAEGEEITEHFDFVDPELPSHAQEIAERGQREVTFLVYLNDDYEGGETAFPRLG
                     ISHKGQRGEGLFFVNALTDGSADVRTLHAGRTPRHGEKWIVSQFVRNRPLL"
BASE COUNT          392 a          737 c          714 g          373 t
ORIGIN      
        1 tcgacgttca cgccggcttc cgtcgcgagt tgcccgatcg tgtagctctg ctgcaccgct
       61 tgactccgta ccatggtacg gccggtatcg taccggaagc tcacccggaa aaatttctaa
      121 ccagtcagat ggataagtgg gagttctttc aggacgccgc aaagctttgg cggtggcgct
      181 gcacgtccgc cgacggccac cgcgtattcg actcggcgct gcgtctcgac aatgacgcgc
      241 cgtaggcgcc gtcgcgcatg gcgaaggtca cggaacacga gctgacggcc ctgaaggcga
      301 aatacctcgc ggcgcactcc gactacgacc gctgcgttgc gacgctctcg caagccggct
      361 tgggctccgc ggctttgctg cccgaggaga tcctgagtcg cttggccaaa gcctttgccg
      421 acttgcagct ggcgcgccac gagtaccgcg ccgcactgtt cgacgtcgcg ttcgccgccg
      481 atgaagcgct gagctgaccg ggtctgtact tcgagcggcc cgtccgtccg tgcgaagacg
      541 gctcggcttc gcggtccggc acgggacggc gtacgcttgg acaacagtca cgaggaagcc
      601 accgtgaaac cgggatacgt cgtactgctc gctgcgagca ccctgctgat ggcgttgccg
      661 tcggccgcgc atcactccgt cgcgatcaac ttcgagcgcg gcgagccgat cgagatccgc
      721 ggcaccgtca aagaggtgca catgcgcaat cctcactcgc agtacgtcgt tagcgtgccg
      781 gccgcggacg gcacggccac cgattggttc gtcgagtggt cggaccggaa ctcgctcgtc
      841 cggcgcggcg tcgatctaga gctcatcaag cccggcgaca cggtgacgtt gacgctgtgg
      901 ccgagccagc gcctgccgaa tgtcggctat ttcgtgcaag ccgtgctggc cgacggctcg
      961 gtctaccgcg attgcggctt cgtccagtac cgggaggccg tggtcaagca gagcaagttc
     1021 cgctgcgaag aaggcacgcg cagcgccccg cagcgagagg cgcaatgaaa atcggcgcag
     1081 cgacggccgc cgcgttactc gcggtcattg cgacggcggc gtcggcggcc ggcacccgca
     1141 gcgacctcga cggcgtgtgg ttgttttacg cgtcgcaagt ccccgacgac cccggcttaa
     1201 cgccggccgg tcgggcgaag ctcgagcagt acgatccgct acgcgacgat gccgacacgg
     1261 actgcaagcc cgtgacgttc acgaacatca tgcacacgcc gtcgccgccg atcgaaatcc
     1321 ggctgcacga ggccgacgtc gagatcaact acgagttcat ggacgtgcac cggcgcgtgc
     1381 cgctcaacgg gaccttggcg acggcgccgc tgaccgtcgc acggcatcag cacatggggc
     1441 gctcgctggg gcgctacgaa ggcgacgagc tcgtcgtcga gaccgtcgac gtcgaggccg
     1501 gttatctcga tacgctcggc ttcgccggct taccgcaatc ggcgcagatg cgcaccgagg
     1561 agcgattcat ccccgacggt gaccgcctca aggtcgtcgt gacgcaccac gaccccgtct
     1621 actacacgtc gccgatggtc atgacctacg acttcgtgcg tgtcgacacc aagatcatgg
     1681 aatggggttg cacgctcgaa ggcgcaaact acctcgaacg gctcaaggac gccgaagcga
     1741 aatagcggcg ccggtcagag aagcggacgg ttgcgcacga actgcgagac gatccatttc
     1801 tcgccgtgcc gcggcgttcg ccccgcgtgc agcgtgcgca cgtccgcgct gccgtcggta
     1861 agcgcgttca cgaagaacaa tccctcgccg cgttgcccct tgtgactgat gcccagccgc
     1921 ggaaacgcgg tttctccgcc ttcgtagtcg tcgttgagat agacgaggaa cgtcacctcg
     1981 cgctgcccgc gctccgcgat ctcctgcgcg tggctcggca gctcggggtc cacgaagtcg
     2041 aagtgctcgg taatctcctc gccctctgca tagtgcagca ccgtggcggc ctcgaaatgg
     2101 cgcagcggcg tgccaaggca agcggccatg cgcagctgcg cgagcacgca aaccaaatcc
     2161 gtgtcgagca gattgaaaac ggcggcggtg ttcgtgcgcg tgggtttcgc catgac
//
LOCUS       DATLEE010000478         5833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1284026, whole genome shotgun sequence.
ACCESSION   DATLEE010000478 DATLEE010000000
VERSION     DATLEE010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284026"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VM692_10345"
     CDS             <1..185
                     /locus_tag="VM692_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52612.1"
                     /translation="STKADLSAYRARAQRFADTLQQLVSQGKSRADIEKVVRGQFDWE
                     DFHVMAALDGLINEFR"
     gene            192..1160
                     /locus_tag="VM692_10350"
     CDS             192..1160
                     /locus_tag="VM692_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUQ52613.1"
                     /translation="MRSYWVVQGERGLTLELRNVAQPEPKEGQLLVKVQASSFNRGEL
                     LTGHGAYDPKGKAAGIECAGEIARLGSGVTRFELGERVMGRCVGGFADYSVMDAREAM
                     RIPQRLSWEQAAAIPLSFMVAHDMLVTHGNLQRGEWLLITGVSSGVGVASLLAGKALG
                     ANVIGTSGSAQKLDRLRELGLDVGVATRSGGFHADVMTATGNKGVDLVVNNVGGTVFA
                     ECVRSLAYQGRLATVGYLDRTLKAEIDLEALHARRLHLFGVSNKLRPAAERAVTVRAF
                     ERDFVPLFADGRLTPVVDRVFSLDDVSAAHAYFESNAMVGKVVVRR"
     gene            complement(1157..2476)
                     /locus_tag="VM692_10355"
     CDS             complement(1157..2476)
                     /locus_tag="VM692_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUQ52614.1"
                     /translation="MLERVSVCALAALALVAASPLAAQDDEAFAERERALARELAEQQ
                     EAAEQRDPEAARELAQQQEESAQRERELTREYEAQQREFESQQRELQAQQAQQQREMA
                     REYEAQQREFASQARELAQQQEQSARELARELANARSELQVSAAEIARLSAELSGKAM
                     GDAVRSVQNDQRVMIQGRGSMLGINIQDTDGGVRVNGVSPNGPAATAGVAVGDTIVAI
                     DGVELDDGAGTAGFLSRMGDVEPGQDVELRIRRGSNGRVVVVRAAENTGRLFQYSPFF
                     RALPAAPAAPAASAAPTAPFRIGPGEFVYAFGGPWNDVELLALTAGLGEYFGTEEGLL
                     VARAGSMAELGLRDGDVILEVGGRQPQSPEHATRIFASFQPGESVPLSIMRQRRRETV
                     TITVPNEPAAWRSGGPWIVRPERAERPERPERPERAEQPEAPERPEL"
     gene            complement(2506..2982)
                     /locus_tag="VM692_10360"
     CDS             complement(2506..2982)
                     /locus_tag="VM692_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52615.1"
                     /translation="MHDSTVDKFKLRELPELSPPADVWDRVLEAERARRGARRFWVRT
                     AAMAAAAAVVAVGVTAYFVNTPDAPQTDAAPYQALLDESARLEQLLAEMPAPRPLMAG
                     STASTIVGLENRIAFVDEQLSYAAADKSASPYRQALWGERVELMNALVYVRFAQAL"
     gene            complement(2964..3530)
                     /locus_tag="VM692_10365"
     CDS             complement(2964..3530)
                     /locus_tag="VM692_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUQ52616.1"
                     /translation="MPNPLPPVAVDTLARARRGDRDAHAELYTRFAGPVFTLARRMLA
                     SKALAEDVLQETFVEVICKIDGFRGEAEVGTWIKRIAINKCLMHVRSSWVSRRVASDE
                     EPVGEEGTSGLEQVVLERALDQVPDVARAVVWLHDVEGYTHAEIGRLMGRTASFSKSQ
                     LARAHERLRSLLEGPEVGSETDLCTTVL"
     gene            complement(3649..4359)
                     /locus_tag="VM692_10370"
     CDS             complement(3649..4359)
                     /locus_tag="VM692_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HUQ52617.1"
                     /translation="MTLVDVSRALSKRVGSLRFAEPVCCVYNPLDYARAPHELYLSRY
                     GSEPKEVLLLGMNPGPFGMAQTGVPFGDVTLVREWLGIVAPVDKPRDEHPKRPVLGFE
                     CRRAEVSGTRLWGWARDRFGTPQRFFERFFVANYCPLAFMESSGANRTPDKLPAAEQQ
                     ALLAVCDEALREVVRALQPRLVIGIGGFAERRVRAALGAANVAIGTILHPSPASPLAN
                     RGWAPTVERQLRELGVAL"
     gene            complement(4397..5641)
                     /locus_tag="VM692_10375"
     CDS             complement(4397..5641)
                     /locus_tag="VM692_10375"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ52618.1"
                     /translation="MFELKLRRLGMGLAVSLALAGAAQAQQQPDLGPRAVTVKDAPYT
                     FDTAELHGIKVSVVARGLAHPFSLAFLPNGDALVSIRSGGLRLVRNAASAKGGAATLV
                     QQPISGTPEAYTQRTSGLHDIVLHPQFAQNGLVYFSYNKLGEPVPDANPPRRQSAITV
                     QRGKLDGMALTNVQEIFTGEWSTGSSGSRLAFGKDGLLYVSTGAPFGMTAAQDPGSVY
                     GKILRLRDDGKPAADNPFAKQAGARPEVFTMGHRDQLGLTVHPNGSVLAAEHGPNGGD
                     EVNLILPGKNYGWPTYTYGRNYDGTRLSAMPVVDGIEQPLVLWVPSIAPTGITVYTGD
                     KIPAWKNNLFVGSARIGEIPRTGGLERVVLNDKLEEIRRERLLGDLHQRVRDVRQGPD
                     GLLYVITDEDDGALLRIEPTAL"
BASE COUNT          891 a         2035 c         1973 g          934 t
ORIGIN      
        1 tctcgacgaa agccgatctc tcggcctatc gcgctcgcgc gcagcgcttc gcggacacgc
       61 tgcagcagct cgtgagccag ggcaagtcgc gcgccgacat cgagaaggtc gtgcgcggcc
      121 agttcgactg ggaagatttc catgtcatgg cggcgctcga cgggctcatc aacgagttcc
      181 gctgaagcgc gatgagatcg tattgggtcg tgcagggcga gcgcggcctc acgctcgagc
      241 tgcgcaacgt cgcgcagccg gagccgaaag agggccagct cctcgtcaag gtgcaggcgt
      301 cgtcgttcaa tcgcggcgag ctgctgacgg gccatggcgc ctacgatccg aaaggtaagg
      361 ccgccggcat cgagtgcgcc ggcgagatcg cacgcttggg cagcggcgtc accaggttcg
      421 agctcggcga gcgcgtcatg ggccgctgcg tcggcggctt cgccgactac tccgtcatgg
      481 acgcgcgcga ggcgatgcgc atcccgcagc gcttgtcgtg ggagcaagcc gcggcaatcc
      541 cactctcgtt catggtcgcg cacgacatgc tcgtcacgca cggcaacctg caacgcggtg
      601 agtggctgtt gatcacgggc gtgtcgtccg gcgtcggcgt ggcctcactc ttggccggca
      661 aggcgctcgg cgccaacgtc atcggcacgt cgggctctgc gcaaaagctc gacagactgc
      721 gcgagctcgg gctcgacgtc ggcgtcgcga cgcgcagcgg cggcttccac gcggacgtca
      781 tgacggccac cggcaacaag ggtgtcgacc tcgtcgtcaa caacgtcggc gggaccgtct
      841 ttgccgagtg cgtccgatcg ctcgcgtacc agggtcggct cgcgaccgtc ggctatctcg
      901 atcgcacctt gaaagccgag atcgatctcg aagcgctgca cgcgaggcgg ctgcatctgt
      961 tcggcgtcag caacaagctg cgtcctgcgg cggagcgtgc cgtgacggtg cgcgccttcg
     1021 agcgcgattt cgtgccgctg ttcgcagacg gccggttgac gccggtcgtc gatcgcgtgt
     1081 tctcgctcga cgacgtctca gccgcacacg cctacttcga gtcgaacgcg atggtcggca
     1141 aggtcgtcgt gcggcgttaa agctccgggc gctcgggagc ttcgggctgc tccgcgcgct
     1201 cgggccgctc cgggcgctcg ggccgctccg cgcgctccgg ccggacgatc catggcccgc
     1261 cggaacgcca ggcggccggc tcgttgggaa cggtgatcgt cacggtctct cggcggcgct
     1321 gccgcatgat cgacagcggc accgactcgc cgggttgaaa gctcgcgaag atccgcgtgg
     1381 cgtgctcggg gctctgcggc tgccggccgc cgacttcgag tatgacgtcg ccgtcgcgca
     1441 agccgagctc ggccatgctg ccggcgcgcg cgacgagcag cccctcctcc gtcccgaagt
     1501 actcgccgag ccccgccgtc aacgccagga gctcgacgtc gttccacgga ccgccgaagg
     1561 cgtagacgaa ttcccccggg ccgattcgga acggagctgt cggcgcggcc gaagccgcgg
     1621 gcgcggcggg cgccgccggt aacgcccgaa agaatggcga gtactggaag agccggcccg
     1681 tgttctcggc cgctctaacg acgacgacgc gcccattgct gccgcgacga atgcgcagct
     1741 cgacgtcttg gccgggttcg acgtcaccca tgcgggacag gaacccggcc gtccctgctc
     1801 cgtcgtcgag ctcgacgccg tcgatcgcga cgatcgtatc gccgacggcc acgccggctg
     1861 tggcagccgg cccgttgggg ctgacgccgt tgacgcgcac tccgccatcg gtgtcctgga
     1921 tgttgatccc gagcattgag ccgcggccct ggatcatgac gcgctggtcg ttttgcacgc
     1981 tgcgcacggc gtcgcccatc gccttgccgg aaagctccgc cgaaaggcgc gcgatctccg
     2041 cggcggagac ttgcagctcc gacctcgcgt tcgcaagctc gcgtgcgagc tcgcgcgccg
     2101 actgctcttg ttgctgcgcg agctcgcgcg cttgggacgc gaactcgcgc tgttgcgcct
     2161 cgtactcgcg cgccatctcg cgttgctgct gcgcttgttg cgcttgcagc tcgcgctgct
     2221 gcgattcgaa ctcgcgttgc tgcgcctcgt actcgcgggt cagctcgcgc tctcgctgcg
     2281 cggactcctc ttgctgctgt gccagctcgc gcgcggcttc tgggtcccgt tgctcggcag
     2341 cctcctgttg ctcggcaagc tcgcgcgcga gcgcgcgctc gcgctccgcg aacgcctcgt
     2401 cgtcctgcgc ggccagcggc gacgctgcaa ccagcgcaag cgcagccaga gcgcacaccg
     2461 atactctctc caacatggtc aatctccgtt gaatctcttt cgatctcaca gcgcctgcgc
     2521 gaagcgcacg tagacgagcg cgttcatgag ctcgacacgc tcaccccaca gcgcctgacg
     2581 gtagggcgac gcgctcttgt cggccgctgc gtacgagagc tgctcgtcga cgaacgcgat
     2641 gcggttctcg agcccgacga tcgtgctcgc cgtcgagccc gccatcagcg gccgaggcgc
     2701 cggcatttcc gcgagcagtt gctcgaggcg ggccgactcg tcgaggagcg cttgataagg
     2761 cgccgcatcg gtctgcggcg catcaggcgt gttgacgaag taagccgtga cgcccacggc
     2821 gacgacggcg gcggcagcgg ccatggccgc cgtgcgaacc cagaaacggc gggcgccgcg
     2881 tcgagcacgc tcggcctcga gcacacggtc ccaaacatcg gctggcggcg agagctcggg
     2941 cagctcgcgc agcttgaatt tgtctacagt actgtcgtgc ataaatccgt ctcgcttcct
     3001 acttccggac cctcgagcaa cgagcgcagc cgctcgtgcg ctcgcgcgag ctgcgacttc
     3061 gagaagctcg ccgtgcggcc catcaaccgg ccgatctcgg cgtgcgtata accttcgacg
     3121 tcgtggagcc agacgacggc gcgcgcgacg tcgggaacct gatcgagcgc gcgctcgagc
     3181 acgacctgct ccaagccgct cgtgccctcc tcaccgacgg gctcctcgtc ggacgcgacg
     3241 cgccgcgaaa cccagctcga ccgcacgtgc atgaggcact tgttgatcgc aatgcgcttg
     3301 atccaagtgc cgacctcggc ctcgccgcga aacccgtcga tcttgcagat gacctcgacg
     3361 aaggtctcct gcagcacgtc ctcggccagc gctttcgacg ccagcatgcg gcgcgccagc
     3421 gtgaaaaccg gcccggcgaa gcgcgtgtag agctcggcat gggcgtcgcg atcgccgcgg
     3481 cgggcgcggg caagggtgtc gacggcgact ggcggcagcg gattcggcat ttgggctccg
     3541 actgcttaga tgccggaggg gcgtaaagcg tcgcaagtcg cgcacgacgt cgatcgcagc
     3601 gagggctatc cctccgtgga gggattgtgc acccgagaga ggcaggcgtc acagcgccac
     3661 gccgagctcc cttagctgcc gctcgaccgt cggcgcccag ccgcgattgg cgagcgggct
     3721 cgcagggctc gggtgcagta tcgtcccgat cgctacgttg gctgcgccga gtgcggcgcg
     3781 cacgcggcgc tcggcgaagc cgccgatgcc gatgacgagc cgcggctgca gcgcccggac
     3841 gacctcgcgc aacgcctcgt cgcacacggc gagcagcgct tgctgctcgg cggccggcag
     3901 cttgtcgggc gtgcggttcg cgccgctcga ctccatgaac gcgagcggac agtaattcgc
     3961 gacgaagaag cgctcgaaga agcgctgcgg cgtgccgaag cgatcgcgcg cccaacccca
     4021 gagccgcgtg ccgctgactt cggcgcgccg acattcgaag ccgagcacgg gccgcttcgg
     4081 gtgctcgtcg cgcggcttgt cgacgggcgc aacgatgccg agccactcgc gcacgagcgt
     4141 cacgtcgccg aacggcacac cggtctgcgc catgccgaac gggcccggat tcatgccgag
     4201 cagcagcact tctttgggtt ccgaaccgta gcggctcaag tacagctcgt gcggagcacg
     4261 agcgtagtcg agcgggttgt agacgcagca aacgggttct gcaaaacgca gagagccgac
     4321 gcgtttcgac aacgcacggc tcacgtcgac gagcgtcatc ggcggcctct gcaccgcgcc
     4381 gatgaagcag ctcgcactac agcgcggtcg gctcgatgcg cagcagcgcg ccgtcatcct
     4441 cgtcggtgat gacatagagc aagccgtcag ggccttgccg cacgtcgcgg acgcgctggt
     4501 gcaggtcgcc gagcaagcgc tcgcgccgga tctcctcgag cttgtcgttg agcacgacgc
     4561 gctcgagacc gccggtgcgc gggatctcgc cgatgcgcgc gctgccgacg aacaggttgt
     4621 tcttccacgc cggaattttg tcgccggtgt agacggtgat gcccgtcggc gcgatcgacg
     4681 gcacccagag cacgagcggc tgctcgatgc cgtcgacgac gggcatcgcc gagaggcgcg
     4741 tgccgtcgta gttgcggccg tacgtatagg tgggccagcc gtagttcttg cccggcagaa
     4801 tcagattgac ctcgtcgccg ccgttcgggc cgtgctcggc cgcgagcacg ctgccgttcg
     4861 ggtgcacggt caacccgagc tgatcgcgat gccccatcgt gaacacctcg ggccgggcgc
     4921 cggcttgctt cgcgaacgga ttgtcggcgg ccggcttgcc gtcgtcgcgc agccgcaaga
     4981 tcttgccgta gacgctgccc ggatcttgcg ccgccgtcat gccgaacggc gcgcccgtcg
     5041 acacgtacaa caagccgtcc ttgccgaacg cgagccgcga accgctcgac cccgtcgacc
     5101 actcgcccgt gaagatctcc tggacgttcg tcaatgccat gccgtcgagc ttgccgcgct
     5161 gcaccgtgat cgcggattgg cggcgcggcg ggttcgcgtc ggggaccggc tcgccgagct
     5221 tgttatagga gaagtacacg agcccgttct gcgcgaactg cgggtgcagc acgatgtcgt
     5281 gcaagccgct cgtgcgctgc gtgtaagcct cgggcgtgcc ggagatcggc tgctgcacca
     5341 gcgtcgccgc cccgcccttc gcgctggcgg cgttgcgcac gagacgtagg ccaccgctgc
     5401 gaatcgatac caacgcgtcg ccgttcggca ggaacgcgag gctaaacggg tgcgccaagc
     5461 cgcgcgccac gacggagact ttgatgccgt gcagctcggc cgtatcgaac gtgtacggcg
     5521 cgtccttgac ggtgacggct cgagggccga gatccggctg ctgttgggcc tgcgcagcgc
     5581 ccgccagcgc cagcgatacc gcgagaccca tacccaaccg ccgcagcttc agctcgaaca
     5641 tcgaatactc cccgccctag tgatgcgaat ggagcgcgag catcgcatcg cgcgcagcgg
     5701 ttgccaagcg ccgcgctttc ggctgtagcc gcgcgccgct aagatgcgcc atggccaccg
     5761 accctgtcgc agggcaccga accgtcgtcc gcgcggcctg cccgcacgat tgccccgaca
     5821 cctgcgcgat gct
//
LOCUS       DATLEE010000479         2802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5012257, whole genome shotgun sequence.
ACCESSION   DATLEE010000479 DATLEE010000000
VERSION     DATLEE010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5012257"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1162
                     /locus_tag="VM692_10380"
     CDS             203..1162
                     /locus_tag="VM692_10380"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HUQ52619.1"
                     /translation="MTKRSGWQRWVRTPQLLGWRRLLVQVHLWVGLGLGLYLVVISVT
                     GSLSVIRPDVHRWFVPRAVATEGTKLTGDALQEAVRRAYPLHEVTQVFERRRADAPVM
                     ITLQKDGATEERLFDPYALRDLGLTYPPITEAIEWVVDLHDNLLADTTGRFVNGIGAL
                     LFVALTITGAIIWWPGVGRVRHNLVPGKPAKSARFARRLHNTLGIWVLALVFIWAVTA
                     VYFSFPDPFEATIDYFDKDLTDVQRPGDWFVRLLVNIHFGRSWGMTVKVLWMVLGLVP
                     AALFITGFVTWWARVVRRRVAEAALPTGGGDPDADLAEEGASS"
     gene            complement(1191..1550)
                     /locus_tag="VM692_10385"
     CDS             complement(1191..1550)
                     /locus_tag="VM692_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52620.1"
                     /translation="MAAGDDTRRVREGAPKKRAASGESAGASDVDSERFRLLSITATR
                     TPAGCSGHDWLVYRIAQGENIITGYRQGDLKTASAEVDAIVTSLNERRRSTKTKPGRK
                     AGGAAAAAQPKEQEPPE"
     gene            complement(1627..2445)
                     /locus_tag="VM692_10390"
     CDS             complement(1627..2445)
                     /locus_tag="VM692_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUQ52621.1"
                     /translation="MSGPLLTRKVHDVLSSAARSGVANVECSLDLDRTKVVVQVAADG
                     WTVAGRRFPYLPRCKDRTIYYWTGAEFEPASRFTTSLIKLVPTQWGAPTFEIDGIKML
                     PSARVSPYADAEGKVRLIEPRGKRILDTCGGLGYFAAWCLQGGAAQITSYEKSADVLW
                     LRSLNPWSPSGAAELTLNHADVASQIDALPPGSFGAALHDPPRFGIAGELYSQRFYAA
                     LARVLLPGAPLFHYTGSPNKLTSGRDVANEVSRRLRSAGFASRLVGDGVLAHKR"
     gene            complement(2469..>2802)
                     /locus_tag="VM692_10395"
     CDS             complement(2469..>2802)
                     /locus_tag="VM692_10395"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ52622.1"
                     /translation="AKLDAAGAKLVLGADTGVEDHLFGLAEHLELEAMVDAGMTPAAA
                     LVAATSRAAEYLHLADRGSLAAGKRADLLVLDASPLDDIANTRRIARVYIGGVEVDRA
                     ALRAQLED"
BASE COUNT          426 a          950 c          939 g          487 t
ORIGIN      
        1 ctgttcagcc gcatcctcga gattcggcgt cggccgatca acgtggtggt tcttgcgaaa
       61 gacggttatc aataacgtcg cgccgatcat tgcacgcctt caatgaaagc gatcgcccaa
      121 cgcgcgctct aaaggtttgc atcgagactc ggcgcgggcg ttgtcgtacc atctcgccgc
      181 tccttcgaca cggaacgcga cgatgaccaa acgatccggc tggcagaggt gggttcggac
      241 gccgcaactc ctcggttggc gccgtttgct cgtgcaagtg cacctctggg tcgggctcgg
      301 tctagggctt tacctcgtgg tcatcagcgt caccggcagc ttgagcgtga tccgcccgga
      361 cgtgcaccgc tggttcgtgc cgcgcgccgt cgcgacggaa ggcacgaagc tgacgggcga
      421 cgccctgcaa gaagcggttc gccgcgctta tccgctccac gaggtaaccc aggtcttcga
      481 gcggcgcaga gcggacgcgc cggtcatgat cacgctgcag aaagacggcg cgaccgagga
      541 gcggctgttc gatccttacg cgcttcgcga cctcgggctc acgtatccgc cgatcaccga
      601 agccatcgag tgggtcgtgg acttgcacga caacttgctt gcggacacga cggggcggtt
      661 cgtcaatggc atcggcgcgc tgttgttcgt cgcgctgacg atcacgggcg cgatcatctg
      721 gtggcccggc gtcggccgcg tccgtcacaa cctcgttccc ggcaaaccgg cgaagtccgc
      781 gcgctttgcg cgccgactgc acaacacgct cggcatctgg gtgctggcgc tggtcttcat
      841 ctgggccgtc acggccgtct acttctcgtt ccccgatccg ttcgaggcga cgatcgatta
      901 cttcgacaag gacctcacgg acgtccaacg gcccggtgac tggtttgtgc gtctgctcgt
      961 gaacatccat ttcggccgct cgtggggcat gaccgtcaag gtgctctgga tggtgctcgg
     1021 cctcgtgccg gcagcgctgt tcatcactgg cttcgtcacg tggtgggcgc gcgtcgtgcg
     1081 ccggcgcgtc gccgaggcgg cgctgccgac cggcggcggc gatcccgacg ccgacctcgc
     1141 cgaagagggc gcttcgagct aagtcctcgt ctcgtacgcc ggcgcgcggc tcattctggc
     1201 ggttcttgtt ccttgggctg cgccgcggcc gctgccccgc ccgccttgcg gccgggcttg
     1261 gtcttcgtcg accggcgccg ctcgttgagc gacgtcacga ttgcatcgac ctcggcactg
     1321 gcggtcttca agtcgccttg ccgatacccc gtgatgatgt tctcgccctg cgcgatccgg
     1381 tacacgagcc agtcgtgacc cgagcagccg gcaggcgtgc gggtcgcggt gatcgacaag
     1441 aggcgaaaac gctcgctatc tacgtcggaa gcgccggcgg actcgccgct cgcggcgcgc
     1501 tttttcggtg cgccttcgcg cacgcgccgt gtgtcatcac cggctgccat ggggcctcac
     1561 gcacattttc gggtgcctta gcgggggaac gccaggaatc tggcggcgac agtgtacagg
     1621 aaacccctag cgcttgtggg ccaggacccc gtcgccgacg agccgcgagg cgaatccggc
     1681 gctgcgcaac cgcctgctga cctcgttcgc gacgtcccgg ccgctcgtga gcttgttcgg
     1741 cgaccccgtg taatgaaaca gcggcgcgcc gggcaatagc actcgagcga gggccgcgta
     1801 gaaccgctgc gagtagagct cgccggcgat tccgaagcgc ggcggatcgt gcagcgcggc
     1861 gccgaacgac ccgggcggca gcgcgtcgat ctggctcgcc acgtcggcgt gattcaacgt
     1921 cagctcggcg gcgccgctcg gcgaccacgg gttcaagctg cgcaaccaca acacgtcggc
     1981 gctcttctcg taggacgtga tttgggcggc cccgccttgc aggcaccagg cggcgaagta
     2041 gccaaggccg ccacaggtgt cgagaattct cttgccacgc ggctcgatca gccggacctt
     2101 gccctccgcg tccgcgtacg gcgacacgcg cgcgctcggc agcatcttga tgccgtcgat
     2161 ctcgaaggtc ggcgcgcccc actgcgtcgg cacgagcttg atcaacgacg tcgtgaagcg
     2221 agacgccggc tcgaactccg cgcctgtcca gtagtagata gttcggtcct tgcagcgcgg
     2281 cagatagggg aaccgccggc cggcgaccgt ccaaccgtcg gctgcgacct gcacgaccac
     2341 cttcgtacgg tcgaggtcga gcgagcattc gacgttagcc acgccggagc gcgcggcgct
     2401 cgagagaacg tcgtgcacct tgcgtgtcag cagcggtccc gacatcgcga acgcgagcag
     2461 cgccgcgctc agtcttcgag ctgcgcgcgc aacgcggctc gatcgacctc gacgccgccg
     2521 atgtagacgc gcgcaatgcg gcgagtattg gcgatgtcgt cgagcgggct cgcgtcgagc
     2581 acgagcaagt cggcgcgctt gccggccgcg aggctgccgc ggtcggcgag gtgtagatac
     2641 tcggcggcgc ggctcgtcgc ggcgacgagc gcagccgcag gcgtcatgcc cgcatcgacc
     2701 atcgcctcga gctcgaggtg ctcggcaaga ccgaacaggt gatcctcgac gcccgtgtcg
     2761 gcgccgagca cgagcttcgc gcccgcggcg tcgagcttgg cc
//
LOCUS       DATLEE010000480         2930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10711856, whole genome shotgun sequence.
ACCESSION   DATLEE010000480 DATLEE010000000
VERSION     DATLEE010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10711856"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VM692_10400"
     CDS             complement(<1..702)
                     /locus_tag="VM692_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUQ52623.1"
                     /translation="MTKQVDIAQLKAFSPIDSLKKDNLAALVKKTKVRDAQPGEALFR
                     EGDAEKRTVYILSGSVELREGAQSVGKVTGGTDDARNPLSPKLPRRHTAVATTGVEYI
                     TIDSDLLDVMLTWDQTGSYEVSELRGENGSGDDWMTTLLQTKAFHKIPPANIQAIFMR
                     LQQVNFKAGDVVIKQGDEGDYFYVITRGKCVVTRETPLNKEGIRLAELQVGDTFGEEA
                     LISEAKRNATVAMETD"
     gene            complement(793..2832)
                     /locus_tag="VM692_10405"
     CDS             complement(793..2832)
                     /locus_tag="VM692_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HUQ52624.1"
                     /translation="MSTSGNPSKHWRRSVDADGVCWLTLDKAGAAANTLSAEVLQELA
                     GELDAVRSASVRGVVFESGKRSGFILGADVKEFGALQDASHATAMAARGQALMDRIAS
                     LDAPTVAAIDGFALGGGLELALACNYRVAVDSYERTLGLPEVQLGIHPGFGGSIRSVQ
                     ILGAPLALDLMLTGRSLSPHEALKHGLVDRVVPRDALRAAAKELIARRPALKRAPWHL
                     SLLNLKPVRGFVAARVRADARKRAKREHYPAPYAIVDLWERYGAHGQQAFRAEAESIG
                     RLLVTDTCRNLVHVFELRERLRNLAPKDSRVRRVHVVGAGVMGGDIAAWCALRGLEVT
                     LQDRAQQYVDPALARARELFAKRLRAPGEADAAGSRLRVDLAAAEAGSADLVLEAIVE
                     KAEAKRGLFADLEPRMSPTALIATNTSSIKLETLASALQHPERFVGLHFFNPVASLPL
                     VEVIRGEQTSDATMMAAMSFVIKIGKLPLPCRSAPGFLVNRLLTPYMLEALYAHVDGH
                     SLESIDAAAKNFGMPMGPVELADRVGLDVALHVAEILSGLLATQPPELLKAKVAKGEL
                     GVKTGRGFYVYDTRGKPIKDRSRAAPDAELADRLLLPLLNEAVACLDEGVVADADLVD
                     AGVVFGAGFAPFTGGPIRYARRRGVAEIVEKLTRFAERFGTRFTPHAGWEKLRNA"
BASE COUNT          497 a          977 c          933 g          523 t
ORIGIN      
        1 gtcggtttcc atggcgaccg tcgcgttgcg cttggcttcc gagatcaacg cttcctcgcc
       61 gaaggtgtcg ccgacctgca gctcggccaa gcgaatgcct tccttgttga gcggcgtctc
      121 gcgcgtcacg acgcacttgc cgcgcgtgat gacgtagaag tagtcgcctt cgtcgccttg
      181 cttgatgacg acgtcgccgg ctttgaagtt cacctgctgc aagcgcatga atatcgcttg
      241 gatgttcgcc ggcggaatct tgtgaaacgc cttcgtctgc agcagcgtcg tcatccagtc
      301 gtcgccgctg ccgttttcgc cgcgaagctc cgagacctcg tacgagcccg tttgatccca
      361 cgtcaacatc acgtcgagca agtcgctatc gatcgtgatg tactccacgc cggtggtcgc
      421 gacggccgta tggcggcgcg gcagcttcgg cgatagagga ttgcgtgcgt cgtccgtgcc
      481 gcccgtaacc ttgccgacgg actgcgcgcc ctcgcgcagc tcgacgctgc ccgagaggat
      541 gtagacggtc cgcttctcgg cgtccccctc gcggaacaac gcctcgcccg gctgtgcgtc
      601 ccgcaccttg gtcttcttga ccagcgcggc caagttgtcc tttttcagac tgtcaatcgg
      661 cgaaaacgcc ttgagctgag cgatgtcgac ttgcttcgtc atcaggggct tagttgcacg
      721 gtctaaatag cggcggcgcc gactagcggc cgaagtctag cacaggggta tcgcgcccca
      781 gtgccgacgg tttcacgcgt tgcgaagctt ttcccacccc gcgtgaggtg tgaaccgcgt
      841 cccaaaacgt tcggcgaaac gggtcagttt ctcgacgatt tccgcgacgc cgcgtcgccg
      901 cgcgtagcga atcggtccgc ccgtgaacgg tgcaaagccg gcgccgaaga cgacaccggc
      961 gtcgacgaga tcggcgtctg cgacgacgcc ttcatccaag cacgcgaccg cctcgttcag
     1021 cagcggcagc agcaaccgat cggccagctc ggcgtcaggc gcggcgcgac tgcgatcctt
     1081 gatcggcttg ccgcgcgtgt cgtagacata gaagccgcga cccgttttga cgccgagctc
     1141 gcctttcgcg actttggcct tcaacagctc gggcggctgc gttgcgagca ggccgctcaa
     1201 tatctcggcg acgtgtaaag cgacgtcgag cccgacacgg tccgcgagct cgacgggccc
     1261 catcggcatg ccgaaatttt tcgccgccgc atcgatgctt tcgagactat gcccgtcgac
     1321 gtgcgcatac agcgcttcga gcatgtacgg cgtcagcagc cgattgacga gaaatcccgg
     1381 cgcgctgcgg cagggcagcg gcagcttgcc gatctttatc acgaacgaca tcgccgccat
     1441 catcgtcgcg tcggacgttt gctcgccgcg gatgacctcg acgagcggca ggctcgcgac
     1501 ggggttgaag aagtgcaacc caacgaaacg ctcggggtgt tgcaatgcgc tcgccaatgt
     1561 ctcgagcttg atgctcgacg tattcgtggc aatcaacgcc gtgggcgaca tgcgaggctc
     1621 gaggtccgcg aacaaaccgc gttttgcttc cgctttttcg acgatcgcct cgagaaccaa
     1681 gtcggcggag ccggcttcgg ccgccgcgag atcgactcgc agccgcgagc ctgcggcgtc
     1741 ggcttcgccg ggcgcgcgca aacgtttggc gaatagctcg cgtgctcgtg ccagtgccgg
     1801 gtcgacgtac tgttgcgcgc ggtcctgcag cgtcacttcg agaccgcgca atgcgcacca
     1861 ggccgcgatg tcgccgccca tcacgccggc gccgacgacg tgcacgcgcc gcacgcgact
     1921 gtccttcggt gcgaggttgc gcaaccgctc gcgcagctcg aacacgtgca cgagattgcg
     1981 gcacgtgtcg gtcacgagca gccggccgat cgattcggcc tctgcgcgga acgcctgctg
     2041 accgtgcgcg ccgtagcgct cccagagatc gacgatcgcg tacggcgcgg gatagtgctc
     2101 gcgcttggcg cgcttgcgtg cgtcggcacg gactcgtgcg gcgacgaagc cgcgaacggg
     2161 cttgagattc agcagcgata gatgccacgg cgcgcgcttg agagcagggc gccgcgcgat
     2221 cagctccttc gccgcagcac gcagggcgtc gcgcggcacg acgcgatcga cgagcccgtg
     2281 cttgagggct tcgtgcggcg agagcgaccg gcccgtcagc atgaggtcga gcgccaacgg
     2341 cgctcccaat atctgcacgg aacgaatcga gccgccgaag ccggggtgaa tgccgagctg
     2401 cacctcgggc agcccgagcg tccgttcgta cgaatcgacg gcgacgcgat agttgcaggc
     2461 aagcgccagc tcgagtccgc cgccgagggc gaaaccgtcg atggcagcca cggtgggcgc
     2521 gtcgagcgac gcgatgcgat ccatcagtgc ttgcccgcgc gccgccatcg ccgtggcgtg
     2581 gctcgcatcc tgtaacgccc cgaactcctt gacgtctgcg ccgagaatga acccgctgcg
     2641 cttgcctgac tcgaagacca cgccgcgcac gctcgccgag cgcactgcgt ccagctcgcc
     2701 ggccagctcc tgcagcactt ccgcggacaa cgtattggcc gctgcgccgg ctttgtcgag
     2761 cgtcaaccaa caaacgccgt cggcgtcgac agagcgacgc cagtgcttcg aagggttgcc
     2821 tgacgtgctc atgctgcaac tcgactctgt ttgtcgatct cgaaatcgca gatcagcggc
     2881 aagtgatctg aaaaccgcac ggcgggtatc gagaaacctg tgacagatcg
//
LOCUS       DATLEE010000481         2313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3266975, whole genome shotgun sequence.
ACCESSION   DATLEE010000481 DATLEE010000000
VERSION     DATLEE010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3266975"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VM692_10410"
     CDS             <1..439
                     /locus_tag="VM692_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUQ52625.1"
                     /translation="VVTQDALAATFDTIFNMITAGLAAIAAISLVVAGVLIMNVMLVA
                     VSQRTAEIGLLKAVGARNRQVLALFLTEAACLSLLGAIVGIVLGAGGIWLMKILFPVL
                     DFAAPPWASLSAVAVAIASGLVFGILPARRAAALDPVNALMRR"
     gene            436..1641
                     /locus_tag="VM692_10415"
     CDS             436..1641
                     /locus_tag="VM692_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52626.1"
                     /translation="MTVRQHDFVRLIGQSVAAHRMRSLLTALGIGIGVTAVVLLTSIG
                     EGLNTYMVEQFTQFGTTTLQVQPGKTSTFGFSPGVLNSVRPLSLGDAEAMTRAPHVLH
                     TVPMVAGSGSVEARGRERSVTVYAVGPDFDKAFKFAVSVGSFLPHDELERARNVAVLG
                     ATLYKELYAGANALGSSIRVGGQRYRVIGVMEPKGDMVGIDLDDTVYIPAARGLELFN
                     RPGLLEIDVLYAEDAPVDEVVAGIKRLLAARHGAEDFTVVTQQQMLDVLGSIVDVLTL
                     GVAALGGISLLVGGVGIFTIMTIAVRERTQEIGLLRAIGARRSVVARLFVGEAMLLSA
                     IGGAGGLALGLSLVLFAQLALPSMPATISPLYVALSLALAVVIGLLAGVLPARSAAQL
                     EPLDALRAE"
     gene            1651..2043
                     /locus_tag="VM692_10420"
     CDS             1651..2043
                     /locus_tag="VM692_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ52627.1"
                     /translation="MSARLNHTIVWCRDQKKSAAFLTEVLGLPPPRRFMHFLVVDLAN
                     DVSVDYYEEKDEPIALQHLAFLVGEDEFDAALVRVRERTAEIWADPAKREPGRINHHF
                     GGRGVYFEDPDGHLLEIITTPYGGASKL"
     gene            complement(2185..>2313)
                     /locus_tag="VM692_10425"
     CDS             complement(2185..>2313)
                     /locus_tag="VM692_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ52628.1"
                     /translation="LHLELGFTYAGTIHHAGFKFGRWLDLAFYELELRTPAMPVDG"
BASE COUNT          341 a          783 c          790 g          399 t
ORIGIN      
        1 ggtcgtgacg caggacgcgc tggctgcaac gttcgacacc atcttcaaca tgatcacggc
       61 cgggctcgcg gccatcgccg cgatcagcct cgtcgtggcc ggcgtgctga tcatgaacgt
      121 catgctcgtc gccgtgagcc agcgcacggc cgagatcggc ttgctcaaag ccgtcggcgc
      181 acgcaatcgc caagtcttgg ctctgtttct cacggaagcc gcgtgtttgt ctctgttggg
      241 cgcgatcgtc ggcatcgtgc tcggcgccgg cggcatttgg ctcatgaaga tcctgttccc
      301 ggtgctcgac ttcgcggcgc cgccttgggc gtccctgtcg gccgtcgccg tggcgatcgc
      361 gagcggcctc gtgttcggca tcctgccggc ccggcgcgcc gcagccctcg atcccgtcaa
      421 cgcgttgatg aggcggtgac agtgcggcag cacgacttcg tacggctgat cggccaatcc
      481 gtcgcggcgc atcgcatgcg cagcctgctg acggcgctcg ggatcggcat cggcgtcacg
      541 gccgtcgttc tgctgacgtc gatcggcgaa ggcttgaaca cctacatggt cgagcagttc
      601 acgcagttcg gcacgacgac gctgcaggtc cagcccggca agaccagcac gttcggcttc
      661 tcgccgggcg tgctgaactc ggtccggccg ttgtcgctcg gcgacgccga ggccatgact
      721 cgcgcgccgc acgtgctgca cacggtgccg atggttgccg gcagcggctc cgtcgaggcg
      781 cgaggccgcg agcgcagcgt cacggtctat gcggtcgggc cggacttcga caaggcgttc
      841 aagttcgccg tgtccgtggg ctcgttcctg ccgcacgacg agctcgagcg cgcgcgcaac
      901 gtggccgtgc tcggcgccac gctgtacaag gagctctacg ccggcgcgaa tgcgctcggc
      961 agcagcatcc gcgttggcgg ccagcgctat cgcgtgatcg gcgtcatgga gccgaagggc
     1021 gacatggtcg gtatcgacct cgacgacacg gtctacattc cggcggcgcg cggcctggag
     1081 ctgttcaatc gccccggcct gctcgagatc gacgtgctgt acgccgagga cgcgcccgtc
     1141 gacgaggtcg tcgccggcat caagagactg cttgcggcgc gccacggcgc cgaggacttc
     1201 acggtcgtga cgcagcagca gatgctcgac gtgctgggct cgatcgtcga cgtgttgacg
     1261 ctcggcgtcg cggcactcgg cggcatctcg ctgctcgtcg gtggtgtcgg catcttcacg
     1321 atcatgacga tcgccgtgcg cgagcggaca caggaaatcg gcctgctgcg cgcgatcggc
     1381 gcgcgccggt ccgtcgtggc gcggctattc gtcggcgaag ccatgctgct gtcggccatc
     1441 ggcggcgccg gcgggctcgc attgggattg agcctcgtgc tgttcgcaca gctggcgttg
     1501 ccgtcgatgc cggccacgat atcgccgctg tacgtcgcgt tgtcgctggc gctggccgtc
     1561 gtaatcggct tgctcgccgg cgtgctgccg gcgcgcagcg cggcgcaact cgagccgctc
     1621 gacgcgctgc gggccgagta gactgacgca atgagcgcac gactcaatca cacgatcgtc
     1681 tggtgccgcg accagaagaa gtcggctgcc ttcctgaccg aggtgctggg cctgccgccg
     1741 ccgcggcgct tcatgcactt cctcgtcgtc gacttggcca acgacgtctc ggtcgactac
     1801 tacgaagaga aagacgagcc gatcgcattg cagcacctcg ccttcttggt cggcgaggac
     1861 gagttcgacg cggccctcgt gcgcgtgcgc gagcgcacgg ccgaaatctg ggccgatccc
     1921 gcgaaaagag agccgggccg catcaaccat catttcggcg gccgcggcgt ctacttcgag
     1981 gaccccgacg gccatctact cgagatcatc acgacgccgt acggcggcgc ctcgaagctc
     2041 tagcccccgc tgtcgccgcg cccgcgacgg caaccgccga cctgcagtgc tcgttcacgc
     2101 cgacggggcg cgattcggat ggcggcacgc tgtcgttcgc catcgtcaac gatccggtga
     2161 cgcggctcag cgccgcacgc gagctcaacc gtcgacgggc atagccggcg tgcgcagctc
     2221 gagctcgtag aaggcaaggt cgagccagcg gccgaatttg aagccggcgt ggtgaatcgt
     2281 gccggcgtaa gtgaaaccga gctcgaggtg cag
//
LOCUS       DATLEE010000482         2537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9489226, whole genome shotgun sequence.
ACCESSION   DATLEE010000482 DATLEE010000000
VERSION     DATLEE010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9489226"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VM692_10430"
     CDS             complement(<1..1026)
                     /locus_tag="VM692_10430"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HUQ52629.1"
                     /translation="MHTLSHVWLPYRQMKTAPPPLAVERTDGVRLYLADGRELIDGIA
                     SWWTACHGYNHPYIRAAVAAQLERLPHVMLGGLVHAPATELAARLAALLPGDLEHVFF
                     SESGSVAVEVAMKIALQYRFNRGEQRRTRFVSFLGGYHGDTFAAMSVCDPEEGMHRSF
                     GAVLPQQLVVPLPTDEASQRDFEQLLEAHAHEVAAVIVEPLVQAAGGMRFHSAETLAR
                     IAAAARRFDLLLILDEIATGFGRTGTMFACEQAGVVPDLITLSKALTGGTLPLAATVA
                     SSRVYEAFFSDDPRHALMHGPTFAGNALACAAANASLDLFEREPRLEQVAAIAAQLEH
                     GLAACRGL"
     gene            1114..2475
                     /locus_tag="VM692_10435"
     CDS             1114..2475
                     /locus_tag="VM692_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009024915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HUQ52630.1"
                     /translation="MKKITCFKAYDVRGRVPEELDDDIAYRIGRAYAAFVKPRTVAVG
                     RDIRNSSPALTAALVKGLTDSGVDVLDIGVGGTELSYFATFSRKLDGGIMVTASHNPP
                     NYNGMKFVREESRPISADTGLQEIRALAERDEFGPPVAARGKVRAVDIMADYIEHLLS
                     YVDVKSLAPLKIVVNAGNGGAGPIVDLLEPHLPFEFIKLYHEPDGSFPNGVPNPMIEE
                     NRVVTSKRLSAERADLAIAWDGDYDRCFLFDELGQFIEGYYIVGLLAQTFLGKSAGER
                     IVHDPRLTWNTLDLVAQGRGKAVQSKSGHAFIKQRMREVDAVYGGEMSAHHYFRGFAY
                     ADSGMIPWLLITQIMSHRKAKLSALVGERMRSFPASGEINRKVKDAPAVIAKVEAEYT
                     PRALAVDRTDGLSCEFASWRFNLRSSNTEPLLRLNVESRGDEALMRAKTAELLALLGG
                     EPG"
BASE COUNT          471 a          851 c          817 g          398 t
ORIGIN      
        1 gagaccgcgg cacgctgcga gcccgtgctc gagctgcgcg gcgatcgccg cgacctgctc
       61 gagccgcggc tcgcgctcga agagatccag cgaggcattc gcggcggcac acgcgagcgc
      121 gttgccggcg aacgtcggcc cgtgcatcag cgcgtgcctg ggatcatcgc tgaagaacgc
      181 ctcgtagacg cgcgagctcg ccaccgtcgc ggcgagcggc aacgtgccgc ccgtcaaggc
      241 cttcgacaac gtgatgagat ccggcacgac accggcctgc tcgcacgcga acatcgttcc
      301 ggtccgaccg aagcccgtgg cgatctcgtc gagtatcagc aatagatcga agcggcgcgc
      361 cgccgcggcg atgcgcgcga gcgtctctgc ggaatgaaat ctcatcccgc ctgccgcctg
      421 cacgagcggc tccacgatca cggccgctac ctcgtgcgcg tgcgcttcta gcagctgctc
      481 gaagtcgcgc tgcgatgctt cgtcggtcgg cagcggcacg acgagctgct gcggcaacac
      541 cgcgccgaac gaccgatgca ttccttcctc gggatcgcag acggacatcg cggcgaacgt
      601 gtcgccgtga tagccgccga ggaagctcac gaagcgcgtg cgtcgctgct cgccgcgatt
      661 gaagcggtac tgcagcgcga tcttcatcgc cacctcgacg gcaaccgagc ccgactccga
      721 gaagaatacg tgctcgaggt cgccgggcag caaagccgca aggcgcgccg ccaattcggt
      781 tgccggcgcg tgcacgagac cgccgagcat cacgtgcggc aagcgctcga gctgcgccgc
      841 cacggcggca cgaatgtacg gatggttata gccgtggcac gcggtccacc acgacgcgat
      901 accgtcgatg agctcgcggc cgtcggcgag atacaagcgg acgccgtcgg tgcgctcgac
      961 ggccaatggc ggcggcgccg tcttcatttg ccgatacggc aaccagacgt gactcaacgt
     1021 atgcacgctc gaacaagcta aactggcatc attgcatagt caggttcgag ttgagcttgc
     1081 ttcggggcgc gaccccttcg gttggatcaa acaatgaaga agatcacttg cttcaaagcg
     1141 tatgacgtcc gcggtcgcgt gcctgaagag ctcgacgacg acatcgccta ccgcatcggc
     1201 cgcgcctacg cggctttcgt caaaccgcgc acggtcgccg tcggacgcga cattcgcaac
     1261 tcgagcccgg cgttgaccgc agcgctcgtc aaaggcctca ccgactccgg cgtcgacgtg
     1321 ctcgacatcg gcgtcggcgg cacagagctg tcgtactttg cgacgttcag ccgcaagctc
     1381 gacggcggaa tcatggtcac ggcaagccac aatccgccga actacaacgg catgaagttc
     1441 gtgcgcgaag agtcgcgccc gatcagcgcg gacacgggcc tacaggagat acgagccttg
     1501 gccgagcgcg acgagttcgg gccgccggtt gcagcgcgcg gcaaggtacg cgcagtcgac
     1561 atcatggccg actacatcga gcacctgctg tcgtacgtcg acgtgaagtc gctagcgccg
     1621 ctcaagatcg tcgtcaacgc cggcaacggc ggtgcgggcc cgatcgtcga tctgctcgag
     1681 ccgcacctgc cgttcgaatt catcaagctc tatcacgagc ccgacggcag cttcccgaac
     1741 ggcgtgccga acccgatgat cgaggagaac cgcgtcgtca cgagcaaacg cctctcggcc
     1801 gagcgcgccg atctcgcgat tgcgtgggac ggcgactacg accgctgttt tctgttcgac
     1861 gagctcgggc agttcatcga gggctactac atcgtcggcc tgctggcgca gacgttcctc
     1921 ggtaagtcgg ccggcgagcg catcgtgcac gacccgcgtt tgacctggaa cacgctcgat
     1981 ctcgtcgctc agggccgcgg caaagccgtg cagagcaaat cgggccacgc attcatcaag
     2041 cagcgcatgc gcgaggtcga tgccgtctac ggcggcgaga tgagcgccca ccactacttc
     2101 cgcgggttcg cgtacgccga tagcggcatg attccgtggc tgttgatcac gcagataatg
     2161 agccaccgca aagcgaagtt gtcggcgctc gtcggcgagc gcatgcgctc ttttccggcc
     2221 agcggcgaga tcaatcgcaa agtcaaagac gcgccggccg tcatcgccaa agtcgaagcc
     2281 gagtacacgc cgcgcgcgct tgccgtggat cgcaccgacg gcttgagttg tgagttcgcg
     2341 agctggcggt tcaatctgcg ctcctcaaac acggagccgc tgctgcgctt gaacgtcgaa
     2401 agccgcggcg atgaggcatt gatgcgcgcg aagactgcgg agctgctggc gctgctcggt
     2461 ggcgagccgg gctagcgcga cgtggcccgc cgtcgcggac tcgcgcgcaa gcgcacccgc
     2521 cgcgcgcact cgacgac
//
LOCUS       DATLEE010000483         4769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9492869, whole genome shotgun sequence.
ACCESSION   DATLEE010000483 DATLEE010000000
VERSION     DATLEE010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9492869"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VM692_10440"
     CDS             <1..789
                     /locus_tag="VM692_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011642290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUQ52631.1"
                     /translation="LAIGQENAFPWIERDLGLTFDTYDVPVVDPQTYQSTRPGVFFGG
                     DAAFGPKNIIWAVQHGHEAAISIHKHCERTSLTERLPRGVTLTTAKMGIHEWSYSNSY
                     EPAARRMMPHVDLKNRFKKLNIEVELGFTAEQFATEVERCLNCDLQTVFTPNLCIECD
                     ACIDICPVDCLAIAPNGDELELSSRLKAARLKPDQPLFVSAPLKQTARVMLKDENLCV
                     HCGLCAERCPTGAWDMQKSTVTVPYARDEARLGEEEWEDNRKAG"
     gene            762..2582
                     /locus_tag="VM692_10445"
     CDS             762..2582
                     /locus_tag="VM692_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HUQ52632.1"
                     /translation="MGRQQKSRINDFVLKIANVNGTGSASANSLLMKTVFRMGIPVSG
                     KNFFPSNIQGLPTWYEIRVSKDGYLARSGAVDIMVAMNAQTYARDLQEVAPGGVLIYD
                     STWPRDRLLSRTDIEIIGVPLARMCNERFANARTRILMKNMAYVGVLAALLELDRAII
                     VELVKETFAKKPALIESNQQAIDLGFEYARANLDCPLPLRLKSLDKTAGHIMIDGNTA
                     AALGAVYAGATVGAWYPITPSTSLMDAFKSFCERYRKDAQSGERRYCVVQAEDELAAI
                     GIVLGATWNGARAFTPTSGPGLSLMSEFLGFAYFTELPAVLFDVQRVGPSTGMPTRTQ
                     QADLLSAAYASHGDTKHVLLFPRDPRECFELAVTAFDLADRLQTPVIVMSDLDIGMND
                     WMCADLDWDPAYRPDRGKVLSAAELEQIKKFHRYVDVDGDGIPQRTLPGVHPRGAYFV
                     RGSGHNQYGGYTEDSVEYQEVVDRLTKKWQTAAKLVPEPVIRRSRQGAKVGVVAVGSC
                     DSAIEEALDLLAEKGIYADYCRIRAFPFGKKVRQFLEQHEHVFVVEQNRDAQLKTLLM
                     AETSVPSTRLQSILHYGGLPMDHRCVTEALTQHAARGVAA"
     gene            2579..3634
                     /locus_tag="VM692_10450"
     CDS             2579..3634
                     /locus_tag="VM692_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HUQ52633.1"
                     /translation="MTSIPKPQIVHQGLKRNALGLTIRDYEGGMSTLCAGCGHDSITA
                     AIVQAFFELDIPPHGVAKMSGIGCSSKTPAYFLSASHGFNSVHGRMPSIATGASAANR
                     NLYVLGVSGDGDSLSIGLGQFMHALRRNLDMVYIIENNGVYGLTKGQFSASADVGSKA
                     KRGEVNKQPPIDPVQMALALGGSFVARSFSGDKEQLVPLIKAGVRHRGFALIDVLSPC
                     VTFNDHEDSTKSYAYAREFNHRAIHADFVPPATEIRAAYDEGDVLPVELHDGSRVLLR
                     KVASDYDARNRGAAIEYIRARQRGGEVVTGLLYIDESHPDMHAVNGTVSAPLSQLPYE
                     QLCPGRDALVALQKRFR"
     gene            3736..4602
                     /locus_tag="VM692_10455"
     CDS             3736..4602
                     /locus_tag="VM692_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52634.1"
                     /translation="MRDRLTGLLADWGAELALVLKELEEKRARVVELEGGAAGRKSEL
                     ESLQQRIDGQNTLIEALRGDAEEASKLRAEVRNKDLEFERVSSELESKKELVRALRRD
                     GDGADRLKSESKQKDRELEELKSKLQRAETKASEATAALAALREATEGKVSEEQAEID
                     ALRSELEARKTVIKSLRADQERVSALQASLEEKRDIIEQLEASINRHSNTIAELRRSV
                     DTWKRKYHAVKGDSPTATTSVNLPSLSDTDVRVIEQIEKASGGKTDATIAIDMRRSLL
                     EARRTASQSNEK"
BASE COUNT          920 a         1494 c         1562 g          793 t
ORIGIN      
        1 ctcgcgatcg gccaggaaaa cgcctttccg tggatcgagc gcgatctcgg ccttacgttc
       61 gacacgtacg acgttcccgt cgtcgatcca cagacgtatc aatcgacgcg gccgggcgtg
      121 ttcttcggcg gcgacgcggc attcggcccc aagaacatca tctgggccgt gcagcacggc
      181 cacgaggcgg cgatctcgat ccataagcac tgcgaacgca cgagcctgac cgagcgccta
      241 ccccgtggcg tgacgttgac gacggcgaag atgggcatcc acgagtggag ctacagcaat
      301 agctacgagc ccgcggcgcg gcgcatgatg ccgcacgtcg acctcaagaa ccggttcaag
      361 aaactgaaca tcgaggtcga gctcggcttc accgcggagc agttcgcgac cgaggtcgag
      421 cgctgcttga attgcgatct gcagacggta ttcacgccga atctgtgtat cgagtgcgac
      481 gcgtgcatcg acatttgtcc cgtcgattgc ctcgcgattg caccgaacgg cgacgaactt
      541 gaactatcgt cgcgtctgaa ggccgcgcgg ctgaagccgg atcagccgct gttcgtgtcg
      601 gcgccgttga aacaaaccgc gcgcgtgatg ctcaaagatg aaaatttgtg tgtgcactgc
      661 gggttgtgcg cggagcgctg cccgaccggt gcgtgggaca tgcagaaatc gaccgtgacg
      721 gttccgtacg cgcgcgacga agcacggctt ggcgaggaag agtgggaaga caacagaaaa
      781 gccggataaa cgacttcgtc ctcaagattg cgaacgtcaa cggtaccggc tcggcgagcg
      841 ccaactcgct gctgatgaag accgtgtttc gcatggggat ccccgtcagc ggcaaaaatt
      901 tcttcccgtc gaacatccag gggttgccga cctggtacga gatcagggtc agcaaggacg
      961 gctatctcgc gcgctcgggc gccgtcgaca tcatggttgc gatgaatgcg caaacctacg
     1021 cgcgcgacct gcaagaagta gcgccgggcg gcgtgctgat ctacgactcc acgtggccgc
     1081 gcgaccggct cttgtcgcgc acggacatcg agatcatcgg cgtgccgctc gcacgcatgt
     1141 gcaacgagcg gttcgcgaac gcgcgcacgc gcatcctcat gaagaacatg gcctacgtcg
     1201 gcgtgttggc cgcgttgctc gagctcgatc gcgcaatcat cgtcgagctc gtcaaggaaa
     1261 cgttcgcgaa gaagccggcg ctgatcgagt cgaaccagca agcgatcgat ctcggcttcg
     1321 agtatgcgcg tgcgaacctc gactgcccgc tgccgctgcg tttgaaatcg ctcgacaaga
     1381 cggccgggca catcatgatc gacggcaata ccgcggccgc gctgggcgct gtctacgccg
     1441 gcgcgacggt cggcgcttgg tatccgatca ccccgtcgac gtcgttgatg gacgccttca
     1501 agtcgttctg cgagcggtac cgcaaggacg cgcaatccgg cgagcggcgg tactgcgtcg
     1561 tgcaggccga agacgagctt gcggcgatcg gcatcgtgct cggcgcgacg tggaacggcg
     1621 cgcgcgcctt cacgccgacg agcggtcccg gcttgtcgct gatgagcgag ttcttgggct
     1681 tcgcttactt cacggaattg ccggccgtgc tgttcgacgt gcagcgtgtc gggccgtcga
     1741 cgggcatgcc gacgcggacg cagcaggccg atctcctgtc cgcggcctac gcatcgcacg
     1801 gcgacacgaa gcatgtgctg ctgtttccgc gcgacccacg cgagtgcttc gagctcgcgg
     1861 tcacggcgtt cgacttggcc gaccggttgc agacgcccgt catcgtgatg tccgatctcg
     1921 acatcggcat gaacgactgg atgtgcgccg atctcgactg ggaccccgcg tatcgccccg
     1981 atcgcggcaa ggttctgtcg gccgccgagc tcgagcagat caagaagttt catcgctacg
     2041 tcgacgtcga cggcgacggc attccgcagc gcacgctccc gggcgtgcac ccgcgcggtg
     2101 cgtacttcgt acgcggctcc ggccacaacc aatacggtgg ctacaccgag gactccgtcg
     2161 agtaccaaga agtcgtcgat cggctgacga agaaatggca aacggccgcg aagctcgtgc
     2221 cggagcccgt gatccgccgc tcgcggcaag gcgccaaggt cggtgtcgtg gccgtgggca
     2281 gctgcgactc ggcgatcgag gaggcgctgg atctactcgc ggagaagggc atctacgccg
     2341 actattgccg catccgcgcg tttccgttcg gcaagaaggt ccggcagttc ctcgagcaac
     2401 acgagcacgt gttcgtcgtc gagcagaacc gcgacgcgca gctcaagacg ctgctgatgg
     2461 cagagacgtc ggttccgagc actcggctgc aatcgatact gcactatggc ggcttgccga
     2521 tggaccatcg ctgcgtcacc gaggcgctca cgcagcacgc cgcacgcgga gtcgcggcat
     2581 gacgtcgatc cccaagccgc aaatcgtgca tcagggcctc aagcgcaacg cgctcggcct
     2641 caccattcgc gactacgaag gcggcatgtc cacgttgtgc gccggttgcg ggcacgactc
     2701 gatcacggcc gcgatcgtgc aggctttctt cgagctcgac attccgccgc acggcgtggc
     2761 caaaatgagc ggcatcggtt gctcgtcgaa gacgccggcg tattttttga gcgcgtcgca
     2821 cggcttcaac tccgtgcacg gacgcatgcc gtcgatcgcg acgggagcga gcgcggcgaa
     2881 ccgcaatctg tacgtgctcg gcgtgtcggg cgacggcgac tcgctgtcga tcgggctcgg
     2941 ccagttcatg cacgcactac gccgcaatct cgacatggtc tatatcatcg agaacaacgg
     3001 cgtgtacggt ctgacgaagg gccagttctc ggcgtcggcc gacgtcggct cgaaggccaa
     3061 gcgcggcgag gtcaacaagc agccgccgat cgatccagtg cagatggcgt tggcgctcgg
     3121 cggctcgttc gttgcacgca gcttctcggg ggacaaagag cagctcgtgc cgctgatcaa
     3181 ggccggcgtg cgacaccgcg gtttcgcact gatcgacgtg ctctcgccgt gtgtgacgtt
     3241 caacgatcac gaagactcca cgaagagcta tgcgtacgcg cgcgagttca atcaccgcgc
     3301 gatccacgcc gatttcgtgc cgccggcaac cgagatcaga gccgcgtacg acgagggcga
     3361 cgtgctgccc gtcgagctgc acgacggcag ccgcgtgctg ctccgcaagg tggcgtccga
     3421 ctacgacgcc cgcaatcgcg gcgccgcgat cgagtacatc cgcgcgcgcc aacgcggcgg
     3481 cgaggtcgtc accgggttgc tgtacatcga cgagtcgcat cccgacatgc atgccgtcaa
     3541 cggcacggtc tccgcgccgc tgtcccagct gccgtacgag cagctctgcc cgggccggga
     3601 cgccctggtg gccctgcaga agcgcttccg gtgattgtga cgtaattcac cgtaatctgg
     3661 ccgccaacac ggcagcgtag ccgtcattcc acgtggtgca tggacatgac ggaacccaca
     3721 gcaacgatcc agggtatgcg agatcgcctg accggcctgc tggccgattg gggtgccgag
     3781 ctcgcgttgg tgctgaagga gctcgaggaa aagcgcgccc gcgtggtaga gctcgaaggc
     3841 ggcgccgcgg ggcgcaagag cgagctcgag tcgctgcagc aacgcatcga cggccaaaac
     3901 accttgatcg aagccttgcg aggcgacgcc gaggaagcct cgaagttgcg tgccgaggtt
     3961 cgcaacaagg acctcgagtt cgagcgcgtg agctcggagc tcgagagcaa gaaagagctc
     4021 gtgcgtgcgc tgcgccgcga cggcgacggc gccgaccggc tgaagagcga atcgaagcaa
     4081 aaggatcgcg agctcgagga gctcaagtcg aagctgcagc gcgccgagac caaggcgagc
     4141 gaggcgacgg cggcgctggc agcgctgcgc gaagcgacgg aaggtaaggt cagcgaagag
     4201 caggccgaaa tcgacgcgct acggtcggag ctcgaagctc gcaagaccgt catcaagagc
     4261 ttgcgcgcgg atcaagagcg cgtcagcgcg ttgcaagcga gcctcgaaga gaagcgcgac
     4321 atcatcgagc agctcgaggc gtccatcaat cgtcactcga acacgatcgc cgagctgcgc
     4381 cgcagcgtcg acacgtggaa gcgcaagtat cacgcggtga agggcgacag cccgacggcg
     4441 acgacgtccg tgaatctgcc gtcgttgagc gacaccgacg tgcgcgtcat cgagcagatc
     4501 gagaaggcgt cgggcggcaa gaccgacgcg acgatcgcga tcgacatgcg ccgctcgttg
     4561 ctcgaggcgc ggcggactgc gtcgcagagt aacgagaagt aacactttcg tacgcccgcg
     4621 tgcggcgcgc tcgcgccgcg tctttgcatt tcttttacta agcgctacgc cgaatgaggc
     4681 gtggcccaac aattgcttct cttgaggcat ggagatgctt ggagcaatct ccgcggctag
     4741 ttatgttcaa cgtttccgat atttgtgac
//
LOCUS       DATLEE010000484         2411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6236425, whole genome shotgun sequence.
ACCESSION   DATLEE010000484 DATLEE010000000
VERSION     DATLEE010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6236425"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /gene="sucB"
                     /locus_tag="VM692_10460"
     CDS             <1..767
                     /gene="sucB"
                     /locus_tag="VM692_10460"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HUQ52635.1"
                     /translation="EHDVLSRAGVSQPEPQPAGGVSRNEHRVAMTRLRARIAERLLQA
                     QTQAAMLTTFNEVDMTGVMALRQRYQEPFTAKHGIKLGFMSFFVKACIEALRQFPAIN
                     ASVDGNDIIYHEFFDIGVAVSTDRGLVVPVLRNAEAMGLGPIESAIRDFGERARKGAL
                     QMEELTGGTFTITNGGIFGSMMSTPILNPPQSGILGMHKIQQRPMVVDGKIEARPMMY
                     LALTYDHRIVDGREAVQFLVHVKDVIEDPARLLLEI"
     gene            790..2217
                     /gene="lpdA"
                     /locus_tag="VM692_10465"
     CDS             790..2217
                     /gene="lpdA"
                     /locus_tag="VM692_10465"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007984586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HUQ52636.1"
                     /translation="MSEKNFDVVVIGGGPAGYPAALRAAQLGLSAACIDDWKNEEGQR
                     LFGGTCLNVGCIPSKALLESSELYHRAHTEFQAHGIHVAGLGVDVEAMQVRKRGIVKQ
                     LTSGIEAMFKAGGVTGYKGRGKLLANNRVEVTTDAGVEILQAQHVVLATGSTPIELPI
                     AKFDGKRIVDSTGALAFAEVPKRLGVIGAGVIGLELGSVWKRLGAEVVILEALDNFLP
                     MADQQIAKDALRHLKKQGLDIRLGAKVTGAAVQGNAVKVRYEAAGAAQDLDVDALVVA
                     VGRRPVTTGLFGADVKVPVDKRGFIEVDEKCRTAVPNVWAVGDVVRGPMLAHKGMEEG
                     VMVAELIAGHVAEMNYKTIPSVIYTAPEIAWVGQTEEEVKAAGRAYKVGTFSFAANGR
                     AKAMQQTAGLVKLIADQEHDDVLGVHIVGPLAGELIAEAVLAMEFSASAEDIQRTIHG
                     HPTLSEALHEASLSADKRAIHGINR"
BASE COUNT          429 a          761 c          814 g          407 t
ORIGIN      
        1 tcgagcacga cgtgttgagc cgcgccggcg tctcgcagcc cgagccgcag cccgccggcg
       61 gcgtctcgcg caacgagcac cgcgtggcca tgacccggct acgtgcgcgc atcgccgagc
      121 gcttgctgca agcgcaaacg caagccgcga tgctgacgac gttcaacgaa gtcgacatga
      181 cgggtgtcat ggcgctgcgg caacgttacc aagagccgtt cacggcgaaa cacggcatca
      241 agctcggctt catgtcgttc ttcgtgaaag cgtgcatcga agcgctgcgc cagtttcctg
      301 cgatcaacgc gtcggtcgac ggcaacgaca tcatctatca cgagttcttc gacatcggcg
      361 tcgccgtctc gacggatcgc ggcctcgtgg tgcccgtgct gcgcaacgcc gaggcgatgg
      421 gcttggggcc gatcgagagc gcgattcgcg atttcggcga gcgtgctcgc aagggcgcgt
      481 tgcaaatgga agagctgacg ggcggcacgt tcacgatcac gaacggcggc atcttcggct
      541 cgatgatgtc gacgccgatc ttgaatccgc cgcaaagcgg catcctcggc atgcacaaga
      601 ttcagcagcg cccgatggta gtcgatggca agatcgaggc gcggccgatg atgtacctcg
      661 ccctgacgta cgatcatcgc atcgtcgatg gtcgcgaggc cgtacaattc ctcgtgcacg
      721 tcaaagacgt gatcgaggat ccggcgcgcc tcctgctcga aatctaatca gccccaataa
      781 agtcaaacaa tgagcgaaaa gaacttcgac gtcgtcgtca tcggcggcgg tccggccggc
      841 tatcccgcgg cgttgcgtgc cgcgcagctc ggcctctcgg ccgcttgcat cgacgactgg
      901 aagaacgagg agggccagcg cctcttcggc ggcacgtgtc tgaacgtcgg ctgcattccg
      961 tcgaaggcct tgctcgagtc gtcggagctc tatcaccgcg cgcacacgga gttccaggcg
     1021 cacggcatcc atgtcgcggg tcttggtgtc gacgtcgagg cgatgcaggt gcgcaagcgc
     1081 ggcatcgtca agcagctgac gtcgggcatc gaagcgatgt tcaaggccgg cggcgtcacg
     1141 ggttacaagg gccgcggcaa gctgctcgcg aataatcgcg tcgaggtgac gacggacgcc
     1201 ggagtcgaaa ttctgcaagc gcagcacgtc gtgctcgcga cgggctcgac gccgatcgag
     1261 ctaccgatcg ccaagttcga cggcaagcgc atcgtcgatt cgacgggcgc gctcgcgttc
     1321 gccgaggtgc cgaagcgctt gggcgtcatc ggcgccggcg tcatcggact cgagctgggg
     1381 tcggtgtgga agcgcctcgg cgccgaggtc gtcatcctcg aggcgctcga taactttctg
     1441 ccgatggccg accagcaaat cgccaaggac gcgctgcggc atctgaagaa gcaggggctc
     1501 gacattcgtc tcggcgcgaa agtcacgggc gccgccgtgc agggcaacgc cgtgaaggtt
     1561 cgctacgagg ccgccggcgc ggcgcaggac ctcgacgtcg acgcgctcgt cgtggccgtc
     1621 ggccgtcgcc ccgtgacgac gggcttgttc ggcgccgacg tcaaagttcc ggtcgacaag
     1681 cgcggcttca tcgaggtcga cgagaagtgc cgcacggccg tgccgaacgt ctgggccgtc
     1741 ggcgacgtcg tccgcgggcc gatgctcgcg cacaagggca tggaagaggg cgtcatggtc
     1801 gcggagctca tcgcgggcca cgtcgccgag atgaactaca agactattcc ttccgtcatc
     1861 tacaccgcgc ccgagatcgc ctgggtcggt cagaccgagg aagaggtcaa agccgcgggt
     1921 cgcgcgtaca aagtcggaac gttcagcttt gcggccaacg gccgcgccaa agccatgcag
     1981 caaacggcgg gcctcgtgaa gctgatcgcg gaccaggagc acgacgacgt cctcggcgtg
     2041 cacatcgtcg gtccgttggc cggcgagctc atcgccgagg ccgtgctcgc gatggagttc
     2101 tctgcgtcgg ccgaggacat tcagcgcacg atccacggtc atccgacact cagcgaggcc
     2161 ttgcacgagg cgtcgctgtc cgccgacaag cgcgccattc acggcatcaa ccgctaacgc
     2221 tcgcccgacg tctgccggta tcattggccg cgggtggctt tgggggccgc cgtcggagta
     2281 gttggagttt gcggtgagtc agcggctggc ccggcgtggt cggtggcgct gtattggtgc
     2341 gacatgcatg tcgtcgaatc cgtgcggatt ctcggaatcg accccgggtc gcagatcacg
     2401 ggtttcggcg t
//
LOCUS       DATLEE010000485         6396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1300424, whole genome shotgun sequence.
ACCESSION   DATLEE010000485 DATLEE010000000
VERSION     DATLEE010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300424"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VM692_10470"
     CDS             <1..765
                     /locus_tag="VM692_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HUQ52637.1"
                     /translation="APRCIIAMERDKPQAMEAIEAAAKVIEDPRLKLAEVPTIYPAGG
                     ERQLVELLTGEEVPSGRYPGEFGYVCQNVGTAYAVQRLHAAREPLTSRIVTITGGGVR
                     TPQNVDAPLGTPIAELVALCGGYSDDVVRLIAGGSMMGFSLPSDDVPTTKATNCVIAA
                     TSTEVRLDPHEWACIRCGDCALVCPSRLLPQDLLVAATSSDYGALATLGLQDCIECGC
                     CDVICPSHIMLTERFRIAKRAVAAHEPIPVTTPGLD"
     gene            769..1803
                     /locus_tag="VM692_10475"
     CDS             769..1803
                     /locus_tag="VM692_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HUQ52638.1"
                     /translation="MRFDQPTAPHARPVVSVPIIMRRVLFALIPAALCHTYFFGSGLL
                     VNFALTATAALLTEAAVLRLRGRNTRYALRDGSALVTAALLSFALPPFVPFWIPLVGG
                     AIAITLAKQLYGGLGKNLFNPAMVGYVFLLLSFPVEMTQWLPPRMGDIDYQRLSLGQH
                     VNYVFSGRLPAGVEIDALTRATPLDLAKEGLRAGRPFAEIRSGSLFGDFGGRGWEWVA
                     NLIALGGLYLLYTGTIRWHIPTAFFAGLLAPATIFYLIDPNGYGAPGFHLFSGASMLG
                     AFFIATEPVSAAGTVRGRLIYGAGIGLLVYTLRTWGGYPDGVAFSVLLMNGAVPLINR
                     YTRPRIYGQR"
     gene            1790..2452
                     /locus_tag="VM692_10480"
     CDS             1790..2452
                     /locus_tag="VM692_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HUQ52639.1"
                     /translation="MANAEGSRLVTVLLAAAIVVAAGIAVTSAYEWSSGRIAANERAR
                     VVARLNSVLDSSLRGRDLTTTRLDITDAELLGSDDPVDVFVVSEAGEPVAVLFASVAP
                     HGYNAAINLLIGVSPAGVVTGVRAVRHRETSGLGDAIESAKSAWIEQFSGKTLASPDA
                     ILWAVQQDDGAFDAITGATVTSRAVVAAVKNTLLYFDRHRSELYAAAAAAAAADDDGR
                     PE"
     gene            2457..3119
                     /gene="rsxE"
                     /locus_tag="VM692_10485"
     CDS             2457..3119
                     /gene="rsxE"
                     /locus_tag="VM692_10485"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HUQ52640.1"
                     /translation="MREATAGLWRNNMTLAQLLGLCPLLAVSTSLVNGLALGVLTTLV
                     LVIANPAMSLMRRVLVPAARIPLYLLLVTALVTSLDMLTHAVLVDLHETLGLFIPLIV
                     VNCGLLAHAETVASRRSVVFVAVSAMATGFGFMFALMALGALREVLGHGTLLAGTGLL
                     RGEDGSGSGLQLPFDGMLVAVLPPGAFFGMGLLLALRNRLLAGSSAKAQAAAPALPEA
                     PR"
     gene            3116..3757
                     /gene="nth"
                     /locus_tag="VM692_10490"
     CDS             3116..3757
                     /gene="nth"
                     /locus_tag="VM692_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HUQ52641.1"
                     /translation="MNAARRRKIYERFARFNPAPATELSYTTPFELLVAVVLSAQATD
                     VSVNKATAKLFPVANTPAALLELGEEGLKIYIKTIGLFNSKARNLMKTCAAIVTQHGG
                     NVPRDRDALEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRVSNRTGLAPGGDVRAVE
                     DELVRVTPDEFKRNAHHWLILHGRYVCKARRPECPRCLIVDLCEYPDKTRELP"
     gene            complement(3842..5623)
                     /gene="lpdA"
                     /locus_tag="VM692_10495"
     CDS             complement(3842..5623)
                     /gene="lpdA"
                     /locus_tag="VM692_10495"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HUQ52642.1"
                     /translation="MSQVEVRIPELGDFPAVDVIEIHVKAGDKIAAEDPLVTLETDKA
                     TMDVPAPQAGTVLSVAIKKGDKVAAGALVLTLEAAGEPAAKAAPPPSAKPAVASPPRA
                     SGAAPARLSAASAAEPKPTAPPAGKRLAVIGAGPGGYTAAFRAADLGLEVTLVERWPT
                     LGGVCLNVGCIPSKALLHAARVIEEAHDMAARGVSFGEVKIDAAKLVGWKASVVRQLT
                     NGLDALAKQRRVRVVRGTARFASANHLEVEREGAAEKIDFDQCIIAAGSEPAALPGIP
                     HDPRIIDSTGALDLKTLPERMLVIGGGIIGLEMATVYDALGVKVSVVELTKTLIPGCD
                     RDLVKPLEKRIAPRYEAIMLGTRFTKMEAGPKGITVHFEGDQAPKEPQTYGLVLVAVG
                     RSANGRKIAADKAGIEVSERGLIHVDKQQRTNVPHIFAIGDIVGQPMLAHKASHEGKV
                     AAEVAAGEKSFFDARVVPAVAYTDPEIAWVGLTEDEAKARGIAYEKGSFPWVASARSL
                     TLGRSEGLTKLLFDAGTKRLLGAGIVGVNAGDLIAEAALAIEMGSDAADIGLTVHPHP
                     TLSETLAFSAEAAEGTITDLYVPKKRH"
     gene            complement(5620..>6396)
                     /locus_tag="VM692_10500"
     CDS             complement(5620..>6396)
                     /locus_tag="VM692_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HUQ52643.1"
                     /translation="EVMHGGAGAASALPSVPSVDFAKYGRVEAEPLSRIQKISGPRLH
                     ASWVNVPHVTQHDEADITELEEKRAALKEQASERGIKLTPLAFIVRACALALAEMPHF
                     KSSLSADGESLVVKHYTHIGFAADTPQGLVVPVIRDADKKDLYEIAKSLATLSERART
                     GKLQSDDMQGGVFTISSLGGIGGKFFTPIINAPEVAILGVSRSAWQPVYRNLAFVPRL
                     TLPLSLSYDHRVIDGAKAVRFTTRLGEILANPGQLVEAIP"
BASE COUNT          981 a         2192 c         2103 g         1120 t
ORIGIN      
        1 gcgccgcgct gcatcatcgc gatggagcgc gacaagccac aggcaatgga agccatcgag
       61 gccgcggcga aagtcatcga agacccgcgc ttgaagctcg ccgaggtgcc gacgatctat
      121 cccgccggcg gtgaacgcca gctcgtcgag ctcttgaccg gcgaggaggt gccgagcggc
      181 cgctatcccg gcgagttcgg ctacgtctgc cagaacgtcg gcactgcgta cgccgtgcag
      241 cgcctgcatg cggcacgcga gcctctgacg tcgcgcatcg tcacgatcac gggcggcgga
      301 gtgcgcacgc cgcagaacgt cgacgcgccg ctcggcacgc cgatcgcgga gctcgtcgct
      361 ctctgcggcg gctacagcga cgacgtcgtc cgcttgattg ccggcggcag catgatgggc
      421 ttttcgttgc ccagcgacga cgtgccgacg accaaagcca cgaactgcgt catcgcagcc
      481 acctcgacgg aagtcaggct cgacccccac gaatgggcat gcatccgctg cggcgattgc
      541 gcgcttgtct gtccctcgcg gctgttgccg caggacctgc tcgtcgccgc gacgagctcc
      601 gactacggcg ccctggcgac gctcggcctg caagactgca tcgagtgcgg ctgctgcgac
      661 gtcatctgcc cgtcgcacat catgttgacg gaacggttcc gcatcgcgaa gcgagccgtg
      721 gcggcgcacg agccgattcc cgtgacgacg ccggggctgg actaatcgat gcgcttcgac
      781 caacccacgg cgccgcacgc tcggccggtc gtcagcgtgc cgatcatcat gcggcgcgtg
      841 ctgttcgcgc tgattccggc tgcgctctgc cacacctact tcttcggctc cgggctgctc
      901 gtcaacttcg cgctgacggc gacggccgcg ctgttgacgg aggccgcggt gttgcgactg
      961 cgcgggcgca acacgcgcta cgcgctgcgc gacggcagcg cgctcgtgac cgccgcgctg
     1021 ttgtcgttcg cgctgccgcc gttcgtgccg ttctggattc cactggtcgg cggcgcgatc
     1081 gcgatcacgc tcgcaaagca gctctacggc gggctcggca agaacctgtt caatccagcc
     1141 atggtcggct acgtgttctt gttgttgtcg ttccccgtcg agatgacgca atggctgccg
     1201 ccgcgcatgg gcgacatcga ttaccagcgg ttgagcctgg gccagcacgt caactacgtg
     1261 ttctcgggaa ggctgccggc cggcgtcgag atcgatgcgc tgacgcgcgc gacgccgctc
     1321 gatctcgcca aggaagggct gcgcgccggg cggccgttcg ccgagattcg cagcggctcg
     1381 ttgttcggcg acttcggcgg gcgcggctgg gagtgggtcg ccaatctcat cgcactcggc
     1441 gggctctatc ttctgtatac cggcaccatt cgctggcata tcccgacggc tttcttcgcc
     1501 gggctcctcg cgcccgccac gatcttctat ctaatcgatc cgaacggtta tggcgcgccg
     1561 ggcttccatc tgttcagcgg cgccagcatg ctcggcgcct tcttcattgc gaccgagccc
     1621 gtctccgctg ccggcaccgt gcgcgggcgg ctgatctacg gcgccggcat cggcctgctc
     1681 gtctacacgt tgcgcacgtg gggcggctat cccgacggag tcgcgttctc ggtcttgctg
     1741 atgaacggcg cagtgccgtt aatcaaccgc tacacgcggc cgcgcatcta tggccaacgc
     1801 tgaaggcagt cggctcgtca cggtgctgct cgccgcggcg atcgtcgtcg cggcgggcat
     1861 cgcagtcacg agcgcctacg aatggagcag cggccgaatc gccgccaacg agcgcgcgcg
     1921 tgtagtcgca cgtttgaaca gcgtgctcga ttcgagcttg cgcggccgcg atctcacgac
     1981 gacgcggctc gatatcaccg acgccgagct gctcggcagc gacgatcccg tcgacgtgtt
     2041 cgtggtgagc gaagcggggg agcccgtcgc cgtgctgttc gcgagcgtcg caccgcacgg
     2101 ctacaacgca gccatcaact tgctgatcgg cgtgtcgccg gccggcgtcg tcacgggagt
     2161 gcgcgccgtc cgccatcgcg agacctcggg cctcggcgac gcgatcgagt ccgcgaaaag
     2221 cgcttggatc gagcagttta gcgggaagac gctcgcgagc cccgacgcga tactgtgggc
     2281 cgtgcagcag gacgacggcg cattcgacgc gatcacgggc gccacggtga cgtcgcgcgc
     2341 cgtcgtcgcc gccgtaaaaa acacgttgct atacttcgac cgacaccgta gcgagcttta
     2401 tgctgctgcg gccgcagcag cggccgccga cgacgatgga cgccccgagt aattccttgc
     2461 gcgaggccac ggccgggctg tggcgcaaca acatgacgct cgcgcagctg ctgggcctct
     2521 gcccgctgct ggccgtcagc acctcgttag tgaacgggct cgcccttgga gtgttgacca
     2581 cgctcgtgct cgtcatcgcg aaccctgcaa tgtcgctgat gcgtcgcgtg ctcgtgccgg
     2641 cggcacggat accgctgtat ctgctgctcg tcacggcgct cgtgacgtcg ctcgacatgc
     2701 tgacgcacgc cgtgctggtc gatctgcacg aaacgctcgg cctcttcatc ccgttgatcg
     2761 tcgtcaactg cggcttgctg gctcatgcgg agaccgtcgc gagccgacga tccgtcgtct
     2821 tcgtcgccgt ctcggccatg gcgacggggt tcggtttcat gttcgcgctg atggcgctcg
     2881 gcgcgctgcg cgaagtgctc ggtcacggca cattgcttgc gggcacaggg ctgctgcgtg
     2941 gcgaagacgg ttcgggaagc ggccttcagc tgccattcga cggcatgctc gtcgcagtcc
     3001 tgccgcccgg cgcttttttc ggcatggggc tgctgctcgc attacgcaac cggctgctgg
     3061 ccgggtcctc ggcgaaggca caagccgccg cgcctgcgct gcccgaggcg ccgcggtgaa
     3121 cgcggcgcgc cgccgcaaaa tctacgaacg tttcgctcgc ttcaacccgg cgccggcgac
     3181 cgagctcagc tacacgacgc cgttcgagct gctcgtggcc gtcgtgttat cggcgcaagc
     3241 gaccgacgtc agcgtcaaca aggcgaccgc gaagctgttt cccgttgcga acacgccggc
     3301 cgcgctgctc gagctcggcg aagaagggct caagatttac atcaagacga tcggcctgtt
     3361 caacagcaag gccagaaatc tcatgaagac gtgcgccgcg atcgtcacgc agcacggcgg
     3421 caacgtgccg cgcgaccgcg acgcgctcga agcgctgccc ggcgtcggcc gcaagacggc
     3481 gaacgtcgtg ttgaacacgg cgttcggcga gccgacgatc gccgtcgaca cgcatatctt
     3541 ccgcgtctcg aaccgcacgg ggctcgcgcc gggcggcgac gttcgcgccg tcgaggacga
     3601 gctcgtgcgc gtgacgcccg acgagttcaa gcgtaatgca caccactggc tgatcctgca
     3661 cggacgctat gtctgcaagg cacgccgacc cgagtgcccg cgctgcctca tcgtcgatct
     3721 ctgcgagtat cccgacaaga cgcgcgaatt gccgtagcac agccgtgcgc cgcgcggggc
     3781 agcgaggtcg gtcgactggg gcggatcggt cgacggcgcc gaccaggagc tgctcgaccg
     3841 gctaatgccg tttcttcggg acgtagagat ccgtgatcgt gccctcggca gcctcggccg
     3901 agaacgccaa cgtctcggac aacgtcgggt gcggatgcac ggtgagacca atgtccgcgg
     3961 cgtcgctgcc catttcgatc gcgagcgccg cttcggcgat caagtcgccg gcgttgacgc
     4021 cgacgatgcc cgcaccgagc aagcgcttcg tgccggcgtc gaacaatagc ttcgtcaacc
     4081 cttccgagcg gccgagcgtc agcgaacgcg cgctcgcgac ccacgggaag ctgcctttct
     4141 cgtaggcgat accgcgagcc ttcgcttcgt cctcggtcaa gccgacccac gcaatttccg
     4201 gatccgtgta cgccacagcc ggcacgacgc gtgcgtcgaa gaagctcttc tcacccgccg
     4261 cgacctcggc cgcgaccttg ccctcgtggc tcgctttgtg cgcgagcatc ggttgcccga
     4321 cgatgtcgcc gatcgcgaaa atgtgcggca cgttcgtgcg ctgctgcttg tcgacgtgga
     4381 tgagtccgcg ctcgctgact tcgatgccgg ctttgtcggc ggcgatcttg cgtccgttcg
     4441 cgctgcggcc gacggcgacg agcacgagac cgtaagtctg cggctccttg ggcgcctgat
     4501 ctccttcgaa atgtaccgtg atgccctttg gcccggcttc catcttcgta aagcgcgtgc
     4561 cgagcatgat ggcctcgtac cgcggcgcga tgcgcttttc gagcggcttg acgagatcgc
     4621 gatcgcaacc cggtatcaat gttttcgtga gctcgacgac ggacaccttg acgccgagag
     4681 cgtcgtacac ggtagccatc tcgagcccga tgatgccgcc gccgatgacg agcatgcgct
     4741 cgggcagcgt cttcaaatcg agggcgcccg tcgagtcgat gatgcgcgga tcgtgcggta
     4801 ttccaggcaa cgccgcgggc tcgctgcccg cagcgatgat gcactgatcg aagtcgatct
     4861 tctcggctgc accttcgcgc tcgacctcga ggtgattcgc ggacgcgaac cgtgccgtgc
     4921 cgcgcacgac gcgcaccctg cgctgcttcg cgagcgcgtc gagtccgttc gtcaactgcc
     4981 gcacgacgct ggctttccag ccgacgagct tcgccgcgtc gatcttcacc tcgccgaacg
     5041 agacgccgcg cgccgccatg tcgtgcgctt cctcgatgac gcgcgcggcg tgcaacagcg
     5101 ctttcgacgg gatgcagccg acgttcaagc agacgccgcc gagcgttggc cagcgctcga
     5161 cgagcgtgac ctcgagcccc aagtccgcgg cgcggaacgc ggcggtgtag cctccaggcc
     5221 ccgcgccgat gacggcaagg cgcttacccg cgggcggcgc tgtcggcttg ggttctgcgg
     5281 ccgacgccgc gctcagacga gccggcgcag cgccgctcgc ccgcggcggg gatgcaacag
     5341 ccggttttgc gctcggaggc ggcgccgctt tggcagccgg ctcgccggcg gcctcgagcg
     5401 tcaacacgag agcgccggcc gcgactttgt cgcccttctt gattgcaacc gacagcaccg
     5461 tgccggcctg cggcgccggc acgtccatcg tcgctttgtc ggtctcgaga gtgacgagcg
     5521 ggtcttcggc ggcgatcttg tcgccggcct tcacgtgaat ctcgatgacg tcgacggctg
     5581 gaaaatcccc gagctcgggg atccgcactt cgacttggct cacggaatcg cctcgacgag
     5641 ctgaccaggg ttcgcgagga tctcgccgag ccgagtcgtg aaccgcacgg ctttggcgcc
     5701 gtcgatgacg cggtgatcgt aagacaacga caacggcagc gtgaggcggg gcacgaacgc
     5761 gaggttccgg tagacgggct gccaggcgct acgcgagaca ccgaggatcg caacctcggg
     5821 tgcattgatg atcggcgtaa aaaacttgcc gccgataccg cccaagctcg agatcgtgaa
     5881 gacgccgcct tgcatgtcgt cggactgcag cttgccggtg cgcgcccgct cgctcaacgt
     5941 cgccaacgac ttcgcgatct cgtaaagatc cttcttgtcg gcgtcgcgga tcacgggcac
     6001 gacgagcccc tgtggcgtat cggccgcgaa cccgatgtgc gtgtagtgct tgacgacaag
     6061 gctttcgccg tcggccgaca gcgacgattt gaagtgcggc atttcggcga gcgcgagcgc
     6121 acaggcgcgc acgatgaacg cgagcggcgt cagcttgatg ccgcgctcgc tggcctgctc
     6181 cttcagcgcc gcgcgcttct cctcgagctc cgtgatgtcg gcttcatcgt gctgcgtgac
     6241 gtgcggcacg ttgacccagc tcgcgtgcag ccgcgggccc gaaatcttct gaatccgcga
     6301 cagcggctcc gcctcgacgc ggccgtattt ggcgaagtcg acgctcggca ccgacggcag
     6361 cgcactggcc gcgccggcgc cgccgtgcat gacctc
//
LOCUS       DATLEE010000486         5403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4260049, whole genome shotgun sequence.
ACCESSION   DATLEE010000486 DATLEE010000000
VERSION     DATLEE010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4260049"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VM692_10505"
     CDS             complement(<1..1218)
                     /locus_tag="VM692_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52644.1"
                     /translation="MNQLKHALRQLKLRPGLSIVVIVMLALGIGATTAMFSLYHQILV
                     RPLPVPEPERLVNLAAPGPGKFGGGYGDLGIGDREAQFSYAMFRELESRQTGFTGLAS
                     HTDFVSNLSYREQPSYSRGGTLVSGRYFEVLNLRPALGRLITPADEPRVGESAVVVLG
                     HDYWQRRFSGDPSVLGQLLTVNGTDLEIIGVAPEGFTGTILGVRADVFVPLSMRWAMV
                     PTLGQYNETNPFRFFNYWTYVFGRLAPGVTLEQANAQLNSLYSGILNDVEAPLIPAQV
                     PQETKDQFRERKIAFSSGTVGWGPLRARAERPLELLIGVTALVLLIVCVNIANLLLAR
                     GAARSGEMAIRASLGAGRFRLARVLLSESLVLIVIGGLTSLFVAALIVRLISGVVPSN
                     QYLGLSSLSPMARL"
     gene            complement(1353..1577)
                     /locus_tag="VM692_10510"
     CDS             complement(1353..1577)
                     /locus_tag="VM692_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52645.1"
                     /translation="MRHGFDVVMRVVTTFVGLIMIMSGCVWMMQGLGVGPAAIMQGFM
                     VNDIHWTFYGAILAIVGVAEIAWAWGFAKA"
     gene            complement(1591..2724)
                     /locus_tag="VM692_10515"
     CDS             complement(1591..2724)
                     /locus_tag="VM692_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52646.1"
                     /translation="MPTGLCNRTILALAVGGLSAAAVAQETASVQVSALRRVPAETTA
                     AAYVDKNWKAPRTSWGHPSFEGAWSTDDFRGVPVTRPREQGTRTSLTQDEFAKRAQEI
                     ESGRDFSVNVGTFLRHEFGLRTFGYTSMIVDPPDGQLPALTPSADAAARAQTRGTFGP
                     GPFDDLDDFSLYDRCITRGVVGSLLPVIYGNGVRITQNPESVAISYEMIHDTRIIPLD
                     GRPKLDSGIRQWMGSARGHFEGDTLVIETTNFTDRTSVGSNGGGVPNTEQLKLTERLT
                     RVDPAMIEYVATIDDPGAFTAPWTLRVMITSRPGYEVLEYSCHEGNGAVGHSLSGERV
                     YERQVAEAKAKGLPIPERAVNHEQIRNGVPGEGQRYFDINAGE"
     gene            2798..3529
                     /locus_tag="VM692_10520"
     CDS             2798..3529
                     /locus_tag="VM692_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006519881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HUQ52647.1"
                     /translation="MRIAVVQTQPVACAIDVNVKAHETWVKKAAAHGADMVFFPELSL
                     TGYEPKHAQRLVLRGDEARLDVFQMLSHALRITIGVGAPTLSLDGVRISMLVFRPDAA
                     RIVYSKQHLHADERAYFVAGDGQVTLASGTDRVAPAICYESLLDEHCAQAVALGANLY
                     VASVAKSAKGVAKAAAHYPAIARRHSLPVLMANCVGACDGFEAAGGSAVWSAAGEMLG
                     CLPATGEGALLFDSTARTAAPVPAS"
     gene            3790..>5403
                     /locus_tag="VM692_10525"
     CDS             3790..>5403
                     /locus_tag="VM692_10525"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HUQ52648.1"
                     /translation="MSKNHSLRSAVRLALATGYGKSTFTATAARRVAQCAILTGAAAG
                     VCLPAYSQDDVDDTIVVTGTRIVQPGVVSSSPIYSIGEQELQRQQEPEVEKILRLLPI
                     TVPGDNQNVNNGTQGASTVNLRGLGSQRNLVLLNGKRAVPFNDDGEVDTSMIPIALIE
                     RIDIVTGGASAVYGSDAISGALNFILRDDFEGVDIQANTSQTAEKDGQDHYFAVTLGT
                     NAADGAANIVLGLNYQERDAVLLGSRPLGQLGIVTADGSGYSDFLAGRAPAAPVAGCG
                     GPGSVAVGGSTTTLPTRVAIAGGPGLGQFRDDGSLQANCGVFNFNPFNFYQTPLERYN
                     ATVLANLDVSDNIEIYSMFNYGKSKVKQQVAHSGIFGNTYFVPLANPLIGAQARTAMI
                     AAANAGRLAGTVNVDGVPDPANPGDFLFHNWIDVNSNGVVDPADELNLSIRRRTVELG
                     TRSEDYNSELFQITMGLRGDLTDSWSYDASVQYGESNRILLRQGYTNLTNVGNALRTT
                     DGVTCENGDPTCVPLNVFGGFGSITPEMAR"
BASE COUNT          951 a         1811 c         1751 g          890 t
ORIGIN      
        1 caaccgcgcc atcgggctca aggacgagag gccgagatac tggttcgacg gcacgacccc
       61 cgagatcaac cgcacgatca gcgcggcaac gaacaggctc gtgagcccgc cgatgacgat
      121 cagaacgagc gattcggaga gcagcaccct ggcgagccgg aagcgcccgg cgccgagcga
      181 cgcgcgaatc gccatctcgc cgcttcgcgc ggcgccgcgc gcgagcaaca ggttcgcgat
      241 gttgacgcag acgatcagca gcacgagcgc cgtgacgccg atcagcagct cgagcggacg
      301 ctccgcgcgc gcgcgcagcg ggccccaacc gacggtgccc gacgagaacg cgatcttgcg
      361 ctcgcgaaat tgatccttgg tttcctgcgg cacttgcgcc ggaatcagcg gcgcttcgac
      421 gtcgttgaga atgccgctgt acaggctgtt gagctgcgcg ttcgcctgct cgagcgtcac
      481 gccgggcgcg agccgcccga agacgtacgt ccagtagttg aagaaccgaa acggattggt
      541 ctcgttgtac tggcccagcg taggaaccat tgcccaacgc atcgacagcg gcacgaacac
      601 gtcggcgcgc acgccgagaa tcgtgcccgt gaagccttcc ggtgccacgc cgatgatctc
      661 gaggtccgtg ccgttgacgg tcaatagctg gccgaggacg ctcgggtcgc cggagaagcg
      721 gcgctgccag tagtcgtgac cgagcacgac gacggccgat tcgccgacgc gcggctcgtc
      781 ggccggcgtg atgagcctgc ccaacgccgg ccgcaggttc agcacttcga agtagcggcc
      841 cgacaccagc gtgccgccgc ggctatagct cggctgctcg cgatagctca ggttcgagac
      901 gaaatccgtg tgggacgcga gacccgtgaa cccggtctgg cgcgattcga gctcgcggaa
      961 catcgcgtag ctgaactgcg cttcgcgatc gccgatgccg aggtcgccgt aaccgccgcc
     1021 gaacttgccg gggcccggcg cggccaggtt cacgagccgc tccggctccg gaaccggcaa
     1081 cggccgcacg agaatctggt ggtacagcga gaacatcgcc gtcgtcgcgc cgatgccgag
     1141 cgcgagcatg acgatgacga cgatcgaaag cccaggacgt agcttgagct gacgcaacgc
     1201 gtgcttgagc tgattcacga tttcccccct cgggtccgcc ggcgaaccgg cacgcgccca
     1261 acccaggcgc caaagtacgc ctgcctactg agatgcgcga cgcacggttt tggttacgcc
     1321 tcccgccgaa tcatcgcgaa aggtgatgcg cgtcaggcct tggcgaagcc ccaagcccag
     1381 gcgatctcgg caacgccgac aatggcgaga atcgcgccgt agaacgtcca gtggatgtcg
     1441 ttgaccatga agccctgcat gatcgcggcg gggccgacgc ccagtccctg catcatccaa
     1501 acgcagccgc tcatgatcat gatcagaccc acgaacgtcg tgacgactcg catgacgaca
     1561 tcgaatccgt ggcgcatgac ttaggggcgc ctactcgccg gcgttgatgt cgaagtaacg
     1621 ctggccttcg ccgggcacac cgttgcggat ctgctcgtga ttgacggccc gctccggtat
     1681 cggcaagccc ttggccttcg cctccgcgac ttggcgctcg tagacgcgct cgccggagag
     1741 cgaatgaccc acggcgccgt tgccctcgtg gcacgagtat tcgagcacct cgtagccggg
     1801 ccgcgacgtg atcatgacgc gcagcgtcca cggcgccgtg aatgcaccgg gatcgtcgat
     1861 cgtcgcgaca tactcgatca tcgccggatc gacgcgcgtg aggcgctccg taagcttcag
     1921 ctgctccgtg ttcggtactc cgccgccgtt cgagcccacg ctcgttctgt cggtgaagtt
     1981 cgtcgtctcg atgacgagcg tgtctccttc gaaatgcccg cgcgcgctgc ccatccactg
     2041 acggatgccg ctgtcgagct tggggcggcc gtcgagcggg atgatgcgag tgtcgtggat
     2101 catctcgtag ctgatcgcca ccgactcggg gttctgtgtg atgcgcacgc cgttgccgta
     2161 gatcacgggc agcagcgagc cgacgacgcc gcgcgtgatg cagcggtcgt acagcgagaa
     2221 gtcgtcgaga tcgtcgaacg gcccgggccc gaacgtgccg cgcgtttgcg cgcgcgcggc
     2281 agcgtcggcg ctcggcgtca acgccggcag ctggccgtcc ggcgggtcga cgatcatcga
     2341 cgtgtagccg aacgtgcgta ggccgaactc gtggcgcaag aacgtgccca cgttgaccga
     2401 gaagtcgcgc ccgctctcga tctcctgcgc gcgcttcgcg aactcgtctt gtgtcagcga
     2461 agtgcgggtg ccttgctcgc gcggccgagt cacgggcacg ccgcggaagt cgtcggtgct
     2521 ccacgcgccc tcgaagctcg gatggcccca cgacgtgcgg ggcgccttcc aattcttgtc
     2581 gacgtacgcg gcggccgtcg tctccgcggg cacgcggcgc aacgccgaga cttgcaccga
     2641 ggcggtttcc tgggcgacgg cggctgccga gaggccgccg acggccagcg cgaggatggt
     2701 tctattgcag aggccggttg gcatgacgca ccccctagcg agacgtcttg cctgactata
     2761 atcgacgcgc gatctcgaga gccaactaaa gacagccatg cgcatcgccg tcgttcaaac
     2821 ccaacccgtc gcgtgtgcga tcgacgtcaa cgtcaaagcg cacgagacgt gggtcaagaa
     2881 ggccgcggcg cacggcgccg acatggtgtt ctttccggag ctctccctga cgggctacga
     2941 gccgaagcac gcacagcgcc tcgtgctgcg cggcgacgaa gcgcggctcg acgtgttcca
     3001 gatgttgagc cacgcgctgc gcatcacgat cggcgtcggc gcgccgacgt tgtcgctcga
     3061 cggcgtgcgc atcagcatgc tcgtgtttcg ccccgacgcc gcgcgcatcg tctattcgaa
     3121 gcagcacttg cacgccgacg agcgggcgta tttcgtcgcc ggcgacggcc aagtgacgtt
     3181 ggcatcggga accgacaggg tcgcgccggc gatctgctac gagtcgctgc tcgacgagca
     3241 ctgcgcgcaa gccgtcgcgc tcggcgccaa cctctacgtc gcgagcgtcg cgaagtcggc
     3301 caaaggcgtc gcgaaggccg ctgcgcacta ccccgcaatc gcccggcggc actcgttgcc
     3361 tgtgctgatg gccaattgcg tcggcgcatg cgacggcttc gaggctgccg gcggctcggc
     3421 cgtctggagc gccgcgggcg aaatgttggg ctgcctgccg gcgaccggcg aaggcgcgtt
     3481 gctgttcgat tcgacggctc gcacggccgc gccagtaccc gcttcgtgag gcccgaagcg
     3541 gctttgcggg tcgaacgtcg cgatttccca acaagcatct gagcgtccgg taagcagcaa
     3601 accagccgtt tttcgcgcga aatccgctgc tgccgaaccg tacggtctgc ccgtgtgttt
     3661 cctgcgacag acagtttctt gtatcctcaa accgtctcgt tgtcggttat ctcgtgggat
     3721 ccggcacagg ggaagttggg tgccttcggg ccgacgacgc taactatggg tcgttgagga
     3781 ggcactctaa tgtccaaaaa tcactcactt cgctcggccg tacggctcgc gcttgcgact
     3841 ggttacggca agtcgacgtt cacggcgacg gccgctcgta gagtggcgca gtgcgcgatc
     3901 ctgaccggag cggcggccgg agtttgctta cctgcctatt cgcaggatga cgtcgacgac
     3961 accatcgtcg tgacgggcac gcgcatcgtg cagcccggcg tcgtctcgtc gagtccgatc
     4021 tacagcatcg gggaacagga gctgcaacgt cagcaagagc cggaggtcga aaagatcctg
     4081 cgcttgctgc cgattaccgt tccgggcgac aaccaaaacg tcaacaacgg cacgcaaggc
     4141 gcatcgacgg tcaacctgcg ggggctcggc tcgcagcgca acctggtcct cctgaacggc
     4201 aagcgcgccg tgccattcaa cgacgacggc gaagtcgaca cgtcgatgat tccgatcgcg
     4261 ctcatcgagc gcatcgacat cgtcacgggc ggcgcctccg cggtctacgg ctccgacgcc
     4321 atctctggcg cactgaactt cattctcaga gatgacttcg aaggcgtcga catccaagcg
     4381 aacacgtctc agaccgccga gaaggatgga caggaccact acttcgccgt cacgctcggc
     4441 accaacgccg ccgacggcgc cgcgaacatc gtgcttggcc tgaattatca ggagcgcgac
     4501 gccgtgctgc tgggctcgcg gcccctcggc cagctcggca tcgtgactgc ggacggcagc
     4561 ggctactccg acttcctcgc cggccgagcg cccgctgcgc cggtggccgg ctgcggcggc
     4621 cccggctcgg tcgcagtggg cgggtccact acgaccctgc cgacgcgcgt cgcgatcgcg
     4681 ggcggtcccg gcctcggcca gttccgcgat gacggctcgc tgcaggcgaa ctgcggcgtc
     4741 ttcaacttca atccgttcaa cttctatcag acgccgctcg agcgctacaa cgctacggtg
     4801 ctcgcgaacc tcgacgtcag cgacaacatc gagatctact cgatgttcaa ctacggcaag
     4861 agcaaggtga agcagcaggt cgcccactcc ggcatcttcg gcaacaccta cttcgtcccg
     4921 cttgcgaatc ctctgatcgg ggcgcaggcc cgtacggcga tgatcgccgc ggcgaatgcg
     4981 gggcgtttgg ccggcacggt gaacgtggac ggcgtgccgg atccggcgaa tcccggcgac
     5041 ttcctgttcc acaattggat cgacgtcaac agcaacggcg tcgtcgatcc ggccgacgag
     5101 ctgaacctat cgatccgccg ccgtaccgtc gagctgggca cgcgttccga ggactacaac
     5161 agcgagctgt ttcagatcac gatgggcttg cgcggcgacc tgaccgacag ctggtcgtac
     5221 gacgcctccg tgcaatatgg cgagtcgaac cgcattctgc tgcggcaggg ctacacgaac
     5281 ctgacgaacg tcggcaacgc gctgcgcacc acggacggcg tcacgtgcga gaacggcgat
     5341 ccgacttgcg tgccgctgaa cgtattcggc ggcttcggca gcatcacacc ggagatggcg
     5401 cgc
//
LOCUS       DATLEE010000487         3169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7727996, whole genome shotgun sequence.
ACCESSION   DATLEE010000487 DATLEE010000000
VERSION     DATLEE010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7727996"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..777
                     /locus_tag="VM692_10530"
     CDS             160..777
                     /locus_tag="VM692_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HUQ52649.1"
                     /translation="MAERRETAIAQRNLKRRRVLQACCGLALCSRAARGDETEDAVAA
                     PQAGDSLVFAYGDRAGQVIAPADVVLGAKQLLAYPKASGTGRIRDGSRLNQLVVVRLS
                     PDKLSAETRMRAADGVVAYSGVCTHTGCDVTDWYGDSLRFKCPCHESEFDPSDGARVV
                     GGPAPWQLAALPLKLVDGALAVAGAFEGRVGFQPPGLDPFGAGPQ"
     gene            796..1629
                     /locus_tag="VM692_10535"
     CDS             796..1629
                     /locus_tag="VM692_10535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ52650.1"
                     /translation="MIVERYLSAQRFLDEAGPWLAVDEAANNLIFALAQSLAGNDHPF
                     HEPIYLAAVKERGQIVGCAVRPPPDHLDLTPMPPGAAALIAADAVAHCPDLEAIGGQA
                     AAASEFAAAWVRLRGGQWRIAHNWSWLVLREVCAPQRSPGALRLAEQEDWGLISAWAP
                     LYMRDTGAAGSVQRFLERRLRTRSLFVWDDDGPKCMASISGFTPNGARISAVFTPEEQ
                     RRKGYASNTVAALSAQALAGGRNHCVLFAESHHEATVRVYENIGYRHLHGTVLVELGI
                     A"
     gene            1710..2741
                     /locus_tag="VM692_10540"
     CDS             1710..2741
                     /locus_tag="VM692_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HUQ52651.1"
                     /translation="MHFTDLGNGKTWAIVLAAGDGTRLRALTANRDGVAVPKQYCSLR
                     AGPSLLREALRRAFRLVPRARTCAVVAAQHSRWWNGRELAASHFVVQPENRGTANGIL
                     LALVRILARDPEARVVVLPADHHVADERVLAQSMSSAVAALVDERADIVMLGMTPERV
                     DPGLGYIEPDGLMKQQLVRIGGFVEKPTRVHATKLIEAGALWNTFIVAANGRKLLEVL
                     RAREPSVVSDMQRCAADVRAVAPSAALRELYRELPVVDFSRDVAQENPTAFHALRVPA
                     CGWSDLGTVDRVREALQRGGRSASAPDAVVDTVSLARALWSERLPAHAVCDLTDELGV
                     RTVEVVPAR"
     gene            complement(2667..>3169)
                     /locus_tag="VM692_10545"
     CDS             complement(2667..>3169)
                     /locus_tag="VM692_10545"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HUQ52652.1"
                     /translation="GLAARGIPVVGLDSLRYFWNARTPQGFAADLDRVLRFYAQHWAK
                     RRAVVIGYSQGADVLPFALPLVSAEAHALIARAVLVAPTRTAAFEVHLTNWFGGNDGE
                     LPVGPEIEKLDPERVVCIYGVDDDEESPCATLATAVRVRPLPGGHHFNRAYAELVREI
                     TDGMSW"
BASE COUNT          470 a         1111 c         1084 g          504 t
ORIGIN      
        1 gccatccgcc ccccttcgcg cgaacgccgt ccgctcacga taatcggtga gccgccgttt
       61 gtcgtggttc tcgtgcgggg ccgcggttca cggcgttgtg ctcccctcta ccggcgccgc
      121 acaatgccca tcggcgacag caattccagg gaagtctgca tggcggaaag gcgtgagacg
      181 gccattgcgc agcgcaatct gaagcggcgc cgtgtgttgc aggcctgttg cggccttgcc
      241 ctgtgcagtc gcgccgcccg cggcgacgaa accgaggacg cagttgcggc gccgcaggcc
      301 ggcgactcgc tcgtgttcgc gtatggcgat cgcgcgggcc aagtcatcgc gccggccgac
      361 gtcgttctcg gggccaagca gctgctcgcc tacccgaagg cttccggcac cgggcgcatc
      421 cgcgacggct cgcgcttgaa ccagctcgtc gtcgtccggc tctcgcccga taagctcagc
      481 gccgagaccc gcatgcgggc ggccgacggc gtcgtggcgt attcgggcgt ctgcacgcat
      541 acgggctgcg acgtcacgga ttggtacggc gactcgctgc gcttcaagtg cccctgccat
      601 gaatccgagt tcgatccgag cgacggcgca cgcgtcgtcg gcggcccggc gccatggcag
      661 ctcgccgcgc tgccgctaaa gctcgtcgat ggagcgcttg cggtggccgg cgccttcgaa
      721 gggcgcgtcg gcttccaacc gccggggctc gatccgttcg gcgccggccc tcagtagcct
      781 cgtgcgggcg gcggcttgat cgtcgagcgt tacctgagcg cacaacgttt cctcgacgag
      841 gccggcccgt ggctcgcggt cgacgaggcc gcgaacaact tgatcttcgc gctagcccag
      901 tcgctggccg gcaacgatca tccgtttcac gagccgatct atctcgccgc cgtcaaagag
      961 cgcggccaaa tcgtcggctg cgcggtgcgg ccgccacccg accacctcga tctgacgccg
     1021 atgccgcccg gggcggccgc gctgatcgcc gccgatgccg tcgcgcattg cccggacctc
     1081 gaggcaatcg gagggcaagc cgcagcggcc agcgagttcg cagccgcgtg ggttcggctg
     1141 cgcggcggcc agtggcgcat cgcgcataac tggagctggc tcgtgctgcg cgaggtatgc
     1201 gcaccgcagc gctcgcccgg cgcactgcgg ctcgcagagc aggaggactg ggggctgatc
     1261 agcgcttggg caccgctcta catgcgcgac accggtgcgg ccggcagcgt gcagaggttc
     1321 ctcgagcggc gcctccgcac gcgttcgttg ttcgtctggg acgacgacgg cccgaagtgc
     1381 atggcgtcga tctcgggatt cacgccgaac ggggcgcgaa tctcggccgt gttcacgccc
     1441 gaagaacagc gccgcaaggg ctatgccagc aataccgtcg ccgcgctgtc ggcgcaggcg
     1501 ctcgccggcg ggcgcaacca ttgcgtgctg ttcgccgaga gccatcacga ggccacggtg
     1561 cgcgtctacg aaaacatcgg ctatcgtcat ctgcacggca ccgtgctcgt cgagctcggc
     1621 atcgcctgat ttgctgcgca gggccaaccg tcagcatgct gtcagtgttc gtaggcagga
     1681 tgccttcacc gtcacggtgg aggatcgcca tgcatttcac ggacctcggc aacggtaaga
     1741 cctgggccat cgtgctcgcg gccggcgacg gtacgcggct cagggcgctc acggctaacc
     1801 gcgacggcgt cgcggtgccg aagcaatact gctcgctgcg cgccggccct tcgttgctgc
     1861 gcgaagcgct gcggcgcgcg ttccgcctcg tgccgcgtgc acgcacgtgc gccgtcgtcg
     1921 ccgcgcagca ttcgcgttgg tggaacgggc gcgagctcgc ggccagccac ttcgtcgtgc
     1981 aacccgagaa ccgcggcacg gccaacggca ttctgctcgc gctcgtgcgc atcctcgcac
     2041 gcgatcccga ggcgcgcgtc gtcgtgctgc ccgccgatca ccacgtcgcc gacgaacgtg
     2101 tgttggccca gtcgatgtcg agcgcggtcg ccgcgctggt cgacgagcgt gccgacatcg
     2161 tcatgctcgg catgaccccg gaacgcgtgg atcccgggct cggctacatc gagcccgacg
     2221 ggctcatgaa gcagcagctg gtacgcatcg gcgggttcgt cgagaaaccg acgcgtgtgc
     2281 acgccacgaa gctcatcgag gccggcgcac tgtggaacac gttcatcgtt gcggccaacg
     2341 gccgcaagct gctcgaggtg ctgcgcgcgc gcgaaccgtc ggtggtatcg gacatgcagc
     2401 gctgcgccgc cgatgtccgc gccgtcgcgc cttcggcggc gctgcgcgag ctctatcgag
     2461 agctacccgt cgtcgacttc tcgcgcgacg tcgcgcaaga aaacccgacg gcgtttcacg
     2521 cgctgcgggt gccggcctgc ggctggagcg atcttggaac cgtcgaccgc gtgcgggaag
     2581 ctctgcaacg cggcggtcgc agcgcttcgg caccggacgc cgtcgtcgac accgtgagcc
     2641 ttgcgcgcgc cttatggtcg gaacggctac cagctcatgc cgtctgtgat ctcacggacg
     2701 agctcggcgt acgcacggtt gaagtggtgc ccgcccggta gcggccgcac gcgcacggcc
     2761 gtagcgagcg tcgcgcacgg cgactcctcg tcgtcgtcga cgccgtagat gcagacgact
     2821 cgctccggat cgagcttctc gatctcaggc ccgacgggca gctctccatc attgccgccg
     2881 aaccagttcg tcaagtggac ctcgaacgca gcggtgcgcg tcggcgcaac cagcacggct
     2941 cgtgcaatga gcgcatgtgc ctcggcggag accaagggca gcgcgaacgg tagtacgtcg
     3001 gcgccttgcg aatagccgat cacgacggca cgccgcttgg cccagtgctg cgcgtagaac
     3061 cgcagcactc ggtcgaggtc cgcggcgaac ccttgcggcg tgcgcgcgtt ccagaaatag
     3121 cgcagcgagt cgagcccgac gaccgggatc ccgcgcgcgg caagccccg
//
LOCUS       DATLEE010000488         2657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9498596, whole genome shotgun sequence.
ACCESSION   DATLEE010000488 DATLEE010000000
VERSION     DATLEE010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9498596"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="VM692_10550"
     CDS             <1..1485
                     /locus_tag="VM692_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52653.1"
                     /translation="GENFTLARCTDGSVVAWGYDGHGQCSLPSLPAGSTLTRLFVGGH
                     HVAALRNDGRIEAWGWNKFGQCNVPRLPAGERCVELATGEAHTLARGSAGSLVAWGDD
                     SAGQCRVPVLAAGVSFVQVAAAKRHSAALCSDGRIVAWGDDAQGQCQVAALPPATSYL
                     EVAAAGNFTLARRSDGELVAWGDNGAGQTNVPARPAGRSFVQLAAGAVHGVARLDDGS
                     LIAWGANASGQCNVPVLPAGTNFVELAGGGANTRARRSDGSVVVFGDDTFGQCAVPAL
                     PAGLRYSGLAASGSSSIVHVLAQRDDGSVVAWGRDDFGECVVPALPVGTSFAELTAGQ
                     RFSVARLEPCPAAFTFCTAKVNSLGCMPTISSTGNSSAAASSGFVVRATDVRNAKPGF
                     LLYSVHGRAAQPFQGGTLCLAPPIKRTPLSSSGGSPNTANDCTGAYSIDLNAFAAGVL
                     GGTPLPALALPGTVVDCQWYGRDNGFAPPDNCSLSDALEYQVCL"
     gene            1566..>2657
                     /locus_tag="VM692_10555"
     CDS             1566..>2657
                     /locus_tag="VM692_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52654.1"
                     /translation="MNTLLVPRKALISVAFALAVSFSSAAAQDRVIGWGSQVFDSAWP
                     AEAFVEIAAGGSHTAALRADGTVATWGDNAFGQCMTPTPPPGERFVALTAGWYHTLAR
                     VSDGSVMAWGNDFYGQCNLPVPQPGETCVGIAAGLYHSLLLDSDGTIAAIGYNNHGQC
                     SVPPLPTGTTYVEIAAGIYHSVARLSDGSIVAWGWNNWQQCDVPALPDSVTYVEVVAG
                     EYHNLARRSDGVVVAWGYNSDGQCDVPVLPAGLSYTALSAGGYTSAARLSDGSYILFG
                     DDSYGQASLPVPAAGSSFVQVKPGWRHTAGLRSDGRVIAWGRNFELQCSVPVPEPSLS
                     FTQATAGFGSNAALQQDGSMLCFGRNDHGQ"
BASE COUNT          396 a          922 c          931 g          408 t
ORIGIN      
        1 ggcgagaatt tcacgctggc gcgctgcacg gacggttccg tcgtcgcgtg ggggtacgac
       61 gggcacggac agtgcagcct tccgtcgctg ccggccggct cgacgctgac gcgcctcttc
      121 gtcggcggcc atcacgtcgc cgcgttgcgc aacgacggcc gcatcgaagc ctggggctgg
      181 aacaaattcg gccagtgcaa cgtgccgcgt ctccccgcgg gcgagcgctg cgtcgagctg
      241 gcgacgggcg aagctcacac gctcgcgcgc ggaagcgccg gctcgctcgt cgcgtggggt
      301 gacgattcgg ccggccaatg ccgtgtgccc gtgctcgcgg cgggcgtgag cttcgtccaa
      361 gtcgccgcgg cgaagcgaca cagcgcggcg ctctgcagcg acggcaggat cgtcgcgtgg
      421 ggcgacgacg cgcaaggcca atgccaggtg gccgctttgc cgccggcgac gagctacctc
      481 gaagtggccg cggccggcaa tttcacgctc gcgcgaagga gcgacggcga actcgtcgcg
      541 tggggcgaca acggcgcggg acaaacgaac gtcccggcgc gacccgcggg ccggagcttc
      601 gtccagctcg ccgccggagc agttcacggc gtcgcgcgtc tcgacgacgg ctcgctgatc
      661 gcgtggggcg cgaatgcgag cggacagtgc aacgtgcccg tgctcccggc cggcacgaac
      721 ttcgtcgagc tcgctggcgg cggcgcgaac acgcgcgcgc gacgcagcga cggcagcgtc
      781 gtcgtcttcg gcgacgacac gttcggccag tgcgccgtgc ccgcgcttcc cgccgggctg
      841 cggtactccg ggttggcggc gagcggcagc tcgagcatcg tgcacgtcct cgcgcagcgc
      901 gacgacggct cggtggtcgc gtgggggcgc gacgacttcg gcgagtgtgt cgtgccggcg
      961 ctgcccgtgg ggacgagttt cgcggagctc accgccggtc agcgcttcag cgtcgcgcgc
     1021 ctcgaaccgt gtcccgcggc gttcaccttt tgcactgcga aagtgaactc gttgggctgc
     1081 atgccgacca tcagctcgac gggcaactcc agcgcggcgg cgagttccgg tttcgtcgtc
     1141 cgcgccacgg acgtgcgcaa cgcgaagccc ggattcctgc tctacagcgt ccatggccgc
     1201 gccgcgcaac cgttccaggg cggcacgctc tgtctcgcgc cgccgatcaa gcgcactccg
     1261 ctttccagct caggcggctc gccgaacacg gcgaacgatt gcacgggcgc gtattcgatc
     1321 gacctcaacg cgttcgcggc cggagtgctc ggcgggacgc cgctgcccgc gctcgccctc
     1381 cccggcaccg tggtcgactg ccaatggtat gggcgcgaca acggcttcgc gccgccggac
     1441 aactgctcgc tctcggatgc gctcgagtac caagtgtgcc tctgacgggc ccctggacgc
     1501 tcgagagcag caggtcgaat cccgaattca ggtgaggatt cgcccggaag cgtacaaata
     1561 cccgcatgaa cacactcctg gtcccgagga aggcgttgat ttccgtcgcg ttcgcactgg
     1621 cggtgagctt ctcgtccgcc gcggcccaag accgcgtgat cggctggggc tcgcaagtct
     1681 tcgacagcgc gtggccagcc gaggcgttcg tcgaaatcgc cgccggcgga tcgcacacgg
     1741 ccgcgctccg cgccgacgga acggtcgcga cgtggggcga caacgccttc ggccagtgca
     1801 tgacgcccac gccgccgcca ggggagcgct tcgttgcgtt gaccgcgggc tggtaccaca
     1861 cgctcgcgcg cgtcagcgac ggttcggtga tggcgtgggg caacgatttc tacggtcaat
     1921 gcaatctgcc cgtgccgcag ccgggggaga cttgcgtcgg catcgcggcg ggcctctacc
     1981 acagtctgct gctggacagc gacggcacga tcgcagcgat cggctacaac aatcacggcc
     2041 agtgcagcgt gcccccgctg ccgacaggca cgacgtacgt cgagatcgcc gcgggcatct
     2101 accacagcgt cgcgcgcctc agcgacggct cgatcgtcgc gtggggttgg aacaactggc
     2161 agcagtgcga cgtgcccgcc ttgccggaca gcgtgacgta cgtcgaagtc gtcgccggcg
     2221 aataccacaa cctcgcgcgg cgcagtgatg gcgtcgtcgt cgcctggggc tacaactccg
     2281 acggtcaatg cgacgtgcct gtgctgccgg cgggattgag ctacaccgcg ctctccgcgg
     2341 gcggatacac gagcgcggct cggctcagcg acggctccta cattttgttc ggtgacgatt
     2401 cgtacggcca ggcgagcttg cccgtgcccg cagcgggctc gagcttcgtc caggtcaagc
     2461 ccggctggcg acacaccgcc ggcctgcgca gcgacggacg cgtgatcgcc tgggggcgca
     2521 atttcgagct gcagtgcagc gtccccgtgc ccgagccgag cttgagcttc acgcaggcga
     2581 ccgctggttt cgggagcaat gccgcgctgc agcaagacgg ctcgatgctc tgcttcggac
     2641 gcaacgatca cggccaa
//
LOCUS       DATLEE010000489         3605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2048049, whole genome shotgun sequence.
ACCESSION   DATLEE010000489 DATLEE010000000
VERSION     DATLEE010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048049"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..>3605
                     /locus_tag="VM692_10560"
     CDS             178..>3605
                     /locus_tag="VM692_10560"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HUQ52655.1"
                     /translation="MSKLLPRTPKLRTRKPHGSALWAATTIAAASAVALPAAAQPHLD
                     GVWSPVYNWPLIAVHAAMTPEGRVLTYGTKSDGTQTGFFSYDLWDPTAGPSGGHTTFN
                     NGTGTDIFCSSQVIMPQSGDILIAGGDNWTGTGTTNTGNNNSTVFDTGDYTLTRSANM
                     NRARWYSSSTVLVNGEVYIQGGSGGADFPEVRQDNGTFRLLSTAGTTGYDSSFPRNFL
                     APDGRIFGYDTTGKMYFVNAAGTGSFTPMGQLPSANAGWTSSAAMFRPGKILQMGGNS
                     NSATVIDINGPTPIATPTQSMSTMRKWVSATVLPDGKVLATGGSEVENQLTGVNTRAE
                     IWNPATGTWRLGPPAVKARLYHSGALLLPDATVLISGGGAPGPQVNTNAEIYYPPYLF
                     ASDGSFADRPRITSWPTTAEAGDSLALEVDSTVARFAIIKTGSTTHSVNMDQRFVELP
                     FTQSGTAVWAQLPARASDTPPGFYLMFALNTAGVPSTAKMLRINIDSTPNTAVDYTAT
                     IGGAGGAAFNLACAADETLVGVHGRYGTNYVNQVGAQCVKVDQFGRWIGDPVKRALTG
                     TTTTGTLFDKVCARDSAVSGFRGASSQYVNQIELQCRALTPSGGLTGTGTFLGSSGGA
                     GGTAQALQACGTENPGYALYGRSGGMVDSFGMLCRTGVITPISTNAMPVVANPGAQTW
                     VAGQAVTLQIMATDGDGDVLTYSASGLPANLAIDAASGVITGTPAAAGAGQAIVNVTD
                     GEETGLAAFNWTVSGTAPLAVQPMPPQVSKLAGSTVGYAATATGGVNVTYKWQFGDGS
                     PETAESASPSIDHVFSAPGIYFVTLTVSDDVVAAPFIQQFVQTIHLPLTAAAARSSTN
                     IAYESRTTGNSRVWVVNQDNNSVSVFDAITYTRLAELAVGLAPRSIAIAPDGRAWIVN
                     RDAATISIVSPTTLTVVQTLTLPYGSQPFGIVFSPAANRALVVLAGSGQLLALDGASG
                     AQLGMLAVGPNPRHVAIDGTGANAYVSRFVTAPQAGEVTAIVQVQGAGAEVLVIDPSA
                     MSLTQTIVLQHSTKIDAENQGAGVPNYLGAVAIAPDGLTAIVPSKQDNIGRGTLRSTA
                     NLNFQNTVRAISSRVDLVGKVEDYSRRIDHDDSGVASAAVYDRYGIYLFVALET"
BASE COUNT          628 a         1214 c         1196 g          567 t
ORIGIN      
        1 agcgtcgaaa ctctttactc agcggcttat gttgcattgg taagtcattg aagcgatatg
       61 cgattagttc agtggctttg ttcttgcttg gtggcggtcg gcgtgcccgg cgcgacctct
      121 gtctatgccg aagcacgcga cgaaaaacac caagaacaac gatcgacaag gcctttcatg
      181 agcaagctcc tcccgcgtac gccgaagctc cggacccgta agccgcacgg gagtgcgctc
      241 tgggcggcaa cgacgatcgc cgcggccagc gccgtggcgt tgccggcggc cgcgcagccg
      301 cacctcgatg gcgtgtggtc gccggtctac aactggccgc tgatcgccgt gcacgcggcg
      361 atgactccgg aagggcgcgt gctgacctat ggcacgaaga gcgacggcac gcagacgggc
      421 ttcttctcgt acgacctgtg ggatccgacg gcggggccga gcggcggcca cacgacgttc
      481 aacaacggca ccggcacgga catcttctgc agctcgcaag tcatcatgcc gcaatccggc
      541 gacatcttga tcgcaggcgg cgacaactgg accggcacgg ggacgaccaa caccggcaac
      601 aacaacagca ccgtcttcga caccggtgac tacacgctga cgcgctcggc gaacatgaat
      661 cgcgcgcgtt ggtattcgtc gtcgacggtg ctcgtcaacg gcgaggttta cattcaaggc
      721 ggcagcggcg gcgccgactt ccccgaggtg cgccaggaca acggcacgtt ccgtttgctg
      781 tcgacggcgg gcacgacagg ctacgactcg agttttccgc gcaacttctt ggcgcccgac
      841 ggccgcatct tcggttacga cacgacgggc aagatgtatt tcgtaaacgc ggccggcacc
      901 ggctcgttca cgccgatggg gcagctgccg tcggcgaacg cgggttggac gtcgagcgcg
      961 gcgatgttcc gccccggcaa gattctgcag atgggcggca actcgaacag cgcgaccgtc
     1021 atcgacatca acggcccgac gcccatcgcg acgccgacgc aatcgatgtc gacgatgcgc
     1081 aagtgggtca gtgccacggt gttgcccgac ggtaaggttc ttgcgaccgg cggcagcgag
     1141 gtcgagaacc agctgacggg cgtcaacact cgcgccgaga tctggaatcc cgcgaccggc
     1201 acctggcggc tcggaccccc cgcagtcaag gcgcgtctct atcattcggg tgcactgttg
     1261 ctgccggatg cgaccgtgct gatctcgggc ggcggcgctc cggggccgca ggtcaacacg
     1321 aacgccgaga tctactatcc gccgtacctc ttcgcgagcg acggctcttt cgcggatcgg
     1381 ccgcgcatca cgagctggcc gacgacggcc gaggccggcg actcgctggc gctcgaagtc
     1441 gacagcacgg ttgcacgctt cgcgatcatc aagacgggct cgacgacgca cagcgtcaac
     1501 atggaccagc ggttcgtcga gctgccgttc acgcagtccg gcacggccgt ctgggcgcag
     1561 ttgccggcgc gcgcgtccga cacgcccccg ggtttctacc tgatgttcgc gctgaatacg
     1621 gccggcgtgc cgtcgactgc aaagatgctg cgcatcaaca tcgattccac gccgaacacg
     1681 gccgtcgatt acacggcgac gatcggcggc gccggcggcg ccgcgttcaa cctcgcgtgc
     1741 gcggcggacg agacgctcgt cggggtgcac ggccgctacg gtacgaacta cgtgaaccaa
     1801 gtcggcgcgc agtgcgtgaa ggtcgatcaa ttcggccgct ggatcggcga tcccgtcaag
     1861 cgcgcgctca cgggcacgac gacgacgggc acgctgttcg acaaagtctg tgcgcgcgac
     1921 tccgcggtca gcggtttccg cggcgcctcg agccagtacg tcaaccagat cgaattgcag
     1981 tgccgcgcgc tgacgccgag cggcggcctc acggggacgg gcacgttctt gggtagcagc
     2041 ggcggtgccg gcggcactgc gcaggcgctg caggcctgcg gcaccgaaaa tcccggctac
     2101 gcgctgtacg gccgctccgg cggcatggtc gacagcttcg gcatgttgtg ccgcacgggc
     2161 gtcatcacgc cgatcagcac gaatgcgatg ccggtcgtcg cgaatcccgg cgcgcagacg
     2221 tgggtcgccg gccaggccgt cacgctgcaa atcatggcga ccgacggcga cggcgatgtg
     2281 ctgacttaca gcgcctccgg cctgccggcg aatcttgcga tcgacgccgc atcgggcgtc
     2341 atcacgggca cgcccgcggc cgcgggcgcg gggcaggcca tcgtcaacgt gacggacggc
     2401 gaggaaacgg gcctcgctgc tttcaattgg acggtctccg gcacggcgcc gctcgcggtt
     2461 cagccgatgc cgccgcaagt ctcgaagctc gcgggcagca cggtcggcta cgccgcgacg
     2521 gcgacgggcg gcgtcaacgt gacctacaag tggcagttcg gcgacggctc gccggagacg
     2581 gcggaatcgg cgtcgccgtc gatcgaccac gtcttctcgg ctcccggcat ctacttcgtg
     2641 acgctgaccg tcagcgacga cgtcgtcgcg gcgccgttca tccaacagtt cgtgcagacg
     2701 atccatctac cgttgaccgc ggccgccgcg cgctcgtcga cgaacatcgc gtacgagagc
     2761 cgcacgacgg gcaactcgcg cgtttgggtc gtcaatcagg acaacaactc ggtcagcgtg
     2821 ttcgacgcca tcacgtacac gcggctcgcg gagctcgccg tcggcttggc gccgcgcagc
     2881 atcgccatcg caccggacgg ccgagcgtgg atcgtgaatc gcgatgcggc gacgatcagc
     2941 atcgtgagcc cgacgacatt gaccgtcgtc cagactctga cgttgcccta cggctcgcag
     3001 ccgttcggga tcgtcttctc gccggcggcg aatcgcgcac tcgtcgtgct cgcgggcagc
     3061 ggccaattgc tcgcgctcga cggcgcgagc ggcgcgcagc ttggcatgct cgccgtcgga
     3121 ccgaatccgc ggcatgtcgc gatcgacggc actggcgcga acgcatacgt ctcgcgcttc
     3181 gtgactgcgc cgcaggccgg cgaggtgacg gcaatcgtgc aggtgcaagg cgccggcgcc
     3241 gaagtgctcg tcatcgatcc gagcgcgatg tcgctgacgc aaacgatcgt gctgcagcac
     3301 agcacgaaga tcgacgccga gaaccaaggc gccggcgtgc cgaactatct cggcgccgtc
     3361 gcgatcgcgc ccgacggtct gacagccatc gtgccgtcga agcaggacaa catcggccgc
     3421 ggcacgttgc gaagcacggc gaacctgaat ttccagaaca ccgtgcgcgc gatcagctcg
     3481 cgcgtcgatc tcgtcggcaa ggtcgaagac tattcgcggc gcatcgacca cgacgactcc
     3541 ggggtggcca gcgccgccgt ctacgatcgc tacggcatct acctgttcgt cgcgctcgag
     3601 acgag
//
LOCUS       DATLEE010000490         6662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2049785, whole genome shotgun sequence.
ACCESSION   DATLEE010000490 DATLEE010000000
VERSION     DATLEE010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049785"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VM692_10565"
     CDS             complement(<1..1061)
                     /locus_tag="VM692_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HUQ52656.1"
                     /translation="MRARARWGITPLLAAPAGAWALGLGDIELQSALNQPLRAEIALS
                     ATADELQGLKVMLAAPDMFERRGIDRPDFLNRLDVRVVTDRSGRSVVQVSSRESIAEP
                     FVTLLIEATWPRGRNIKEYTVLLDPPVFLPAPAAPPAVQPAETRTSGNNAPGGQINRP
                     TPAAPSAERAPAPAPAPAPAVREPATEPPVSRAAPPAPRAPVAATGGSYGPVQRAETL
                     WAIADRLRPDGVTINQMMIAIYQTNPAAFGGNINVLRAGSTLRLPAAADFDALVATVA
                     NAEVQRQTDEWQNRAPGGQLRLLPPTETAVARAPTPAPAPAITPSTTTRPAEASRPAA
                     NAGAADPAVAAGVEESRRLL"
     gene            complement(1388..2488)
                     /gene="aroC"
                     /locus_tag="VM692_10570"
     CDS             complement(1388..2488)
                     /gene="aroC"
                     /locus_tag="VM692_10570"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007147050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HUQ52657.1"
                     /translation="MAGDTYGRLFTVTTFGESHGPALGCVVDGCPPGLGLTEADIQKD
                     LDRRRPGRSRHVTQRQEPDQVKILSGVFEGRTTGTAIGLLIENVDQRSGDYSKIKDSF
                     RPGHADYTYQQKYGIRDYRGGGRASARETAMRVAAGAIARKYLAEQLGLQIRGYLGEI
                     GPFKLRALDLEHVDDNAFFCADPDKIPQLEVFMDELRRAGDSIGARINLIASGVPVGL
                     GEPVFDKLEGEIAKGLMSINAVKGVEFGDGFECVRQRGSEHRDELTPDGFKKNSSGGT
                     LGGISSGQDILVSIALKPTSSIQVPGATVDKEGRATEIVTTGRHDPCVGLRATPIAEA
                     MLALTLMDHYLRHRGQNADVHSSVPVLAAKQS"
     gene            complement(2562..3467)
                     /gene="prmB"
                     /locus_tag="VM692_10575"
     CDS             complement(2562..3467)
                     /gene="prmB"
                     /locus_tag="VM692_10575"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:TIGR03533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HUQ52658.1"
                     /translation="MSAGDLERRLRAARDCDAWIAVLADYFDAHRLVFGHGTDNAADE
                     AFWLLRHLQDWRDVDFGAPPAAELVPRALAIAERRVDERQPLAYLINEAWFAGLKFFV
                     DERVLVPRSPLAEVLEREFAPWTALRDGDRVLDIGTGSGCIAIAAAHYCAGVTVDATD
                     ISAAALEVAARNVAQHGLGSRVRLFEADLFPPAAERYRVIVSNPPYVPEAEVAALPAE
                     YRHEPELGLASGPTGFAAAERILRGALDRLTADGVLFLELGAGAERFAAANARLPLIA
                     LAFERGGDGVLVTTAAELRQFLHAS"
     gene            3597..4223
                     /locus_tag="VM692_10580"
     CDS             3597..4223
                     /locus_tag="VM692_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HUQ52659.1"
                     /translation="MLRILVVALVVLVAAMFLLPRAQRGEAPQNATVLPEPRALADVR
                     FVDKTGREAHLSDFKGDFTLLFFGFTNCPDVCPLTLSLLKEVRADVAKRAPRLTPRVL
                     FVSVDPKRDTPERIAAYLNGFDPEFLGVTASDEELAPLLQTLGVAVEKHDHGGADYNV
                     VHNSAIYVLDPNADWIAVSTGPHDPQVVASDYLRIRQRRSGGPASPGA"
     gene            4331..5068
                     /gene="asd"
                     /locus_tag="VM692_10585"
     CDS             4331..5068
                     /gene="asd"
                     /locus_tag="VM692_10585"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HUQ52660.1"
                     /translation="MTRWFAKTYGVDLTEAANADLDSYATFNEFFTRELKAGARPIAS
                     EAGTLVAPADGVLTEHGAVDGGRVYQAKGSAYTLRELLAESGAAVSQLEGGRYFTIYL
                     APHNYHRVHAPLAARLTRTRYIPGARFSVSRATAATIPKIFCRNERVVCWFETDHGPM
                     AVVLVGALNVSSISTFAHGEISSGAPREWHEPQPRPVERGGEIGRFNMGSTVVVLLGS
                     SAFEFVGTACDGLAVRMGQALGAFTRA"
     gene            5073..6035
                     /gene="epmA"
                     /locus_tag="VM692_10590"
     CDS             5073..6035
                     /gene="epmA"
                     /locus_tag="VM692_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HUQ52661.1"
                     /translation="MSYDLGASLATLRHRAAMLAAIRSFFAARAVLEVETPALSPAGV
                     TDPAIESIVAEARCLVPARHYLQTSPEFAMKRLLADGSGDIYQLCRVYRDDELGRWHQ
                     PEFTLLEWYRVGWDEQRLMTEVAELIAAACAAAGSAFRSTVRLTYAQALEAALGVGPE
                     APAGELARRLAGKGVDVPPGLAHDAVLDLAFGTVVPASFDARTLTFVYDYPASQAALA
                     RLKLTTPRVAARFEAFSAGIELANGFHELADAAEQRRRFVAERERRVANGQHAPPLDE
                     QLLEALAKLPDCAGVALGFDRLVALATGHDNVAAVMSFAHGRDS"
     gene            complement(6040..6609)
                     /gene="efp"
                     /locus_tag="VM692_10595"
     CDS             complement(6040..6609)
                     /gene="efp"
                     /locus_tag="VM692_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HUQ52662.1"
                     /translation="MAVYSTNEFKSGLKILLDGDPYAILENEYVKPGKGQAFNRVKIR
                     NLKTGRVVERTFKSGDTAEGADVVDTDMQYLYTDGQFWYFMVPDTFEQIAAGEAAVAD
                     AKPWMKEQEMVIVTLWDGVPLYIATPNHVDLKIVETDPGVRGDTATGGQKPAKLETGA
                     TVRVPLFINEGEVIKVDTRSGTYISRVKQ"
BASE COUNT         1022 a         2267 c         2255 g         1118 t
ORIGIN      
        1 agcagccggc ggctctcctc gacacccgcg gcgacggcgg ggtcggccgc accggcgttc
       61 gctgccgggc gcgacgcctc ggcgggccgc gtcgtcgtcg acggtgtgat cgcaggcgcg
      121 ggcgccgggg ttggggcacg agcgactgcg gtctcggtcg gcggcagcag acgcagctga
      181 ccgcccggcg cgcgattctg ccattcgtcg gtttggcgct ggacttcggc gttcgcgacc
      241 gtcgcgacca acgcgtcgaa gtccgcggct gcgggcaagc gcaacgtcga gcccgcgcgc
      301 agcacgttga tgttgccgcc gaaggcggcg ggattcgttt gatagatcgc gatcatcatt
      361 tgattgatcg tgacgccgtc aggccgcaag cggtccgcga tcgcccacag cgtctccgcg
      421 cgttgcacgg gaccgtagct gccgcccgtc gccgccaccg gcgcgcgagg tgcgggcggc
      481 gcagcgcggg acaccggagg ctcggtcgca ggctcgcgca cggccggcgc cggcgcgggt
      541 gcgggcgcag gtgcgcgctc cgccgacggt gccgccggag tcggccgatt gatctggccg
      601 ccgggcgcat tgttacccga ggtgcgagtt tcggccggtt gcacggccgg cggtgcggcc
      661 ggggccggca agaacaccgg cgggtcgagc aggacggtgt attccttgat gttgcggccg
      721 cgcggccaag tcgcctcgat gagcagcgtc acgaacggct cggcgatcga ttcgcgagac
      781 gagacctgca cgacgctacg gccgctgcgg tccgtcacga cgcggacgtc gagacgattc
      841 agaaagtcag ggcggtcgat gccgcggcgc tcgaacatgt cgggagcggc caacatgacc
      901 ttgagccctt gcagctcatc ggccgtcgcc gacaaagcga tttcggctcg gagcggctga
      961 ttgagcgcgg actgaagctc gatgtcgccg agcccaagcg cccatgcccc agcgggagcg
     1021 gccagcagtg gcgtgatgcc ccaacgtgcc cgtgctctca tgcaatctcc ctagctactc
     1081 ggccgtccca tggcggcctc acccgtggcg gggtgggacc gttcgtaacg gaccgaacga
     1141 taccttacgt aaaatggatt taccaaagtc agcttgcctc gcgagcgacg cggttcgcag
     1201 cgcgaaaccg cggcgtgccg agagacatcc ggcgcggaat ttaacaattt gcgggcccag
     1261 cggactgtga ttctcgttcg actttcgaaa cggcgcgcct gacgcggacg gcgactgttg
     1321 tacttaccat aatactcgcg cccgccgggc ggtggcgagc ttcgcgaacc ggccgcttgg
     1381 acgcgagcta gctttgcttg gccgcgagca cggggacgct ggaatgcacg tcggcgttct
     1441 ggccgcgatg ccttaaataa tgatccatca gcgtcaacgc gagcatggct tccgcgatcg
     1501 gcgtggctcg caggccgacg cacgggtcgt gccggcccgt cgtcacgatc tccgtcgcac
     1561 ggccttcctt atctacggtc gctccaggga cttggatgct cgacgtcggc ttcagcgcga
     1621 tgctgacgag aatgtcttgg ccgctcgaga tgccgcccaa ggtgccgccc gacgagttct
     1681 tcttgaagcc gtcgggcgtc agctcgtcgc gatgctcgct gccgcgttgc cgcacgcact
     1741 cgaagccgtc gccgaactcg actcctttca cggcgttgat gctcatgagg cctttggcga
     1801 tctcgccctc gagcttgtcg aacacgggct cgcccaagcc gacgggtaca cccgaggcga
     1861 tcaagttgat gcgtgcgccg atcgagtccc ccgccctgcg cagctcgtcc atgaatacct
     1921 cgagctgcgg aatcttgtcg gggtccgcac agaagaacgc attgtcgtcg acgtgctcga
     1981 gatcgagcgc gcgcagcttg aacggcccga tctcgccgag gtagccgcgg atctgcaaac
     2041 cgagctgctc ggcaagatac ttgcgcgcaa tcgcgccggc cgccacgcgc atcgccgttt
     2101 cacgggccga tgctcggccg ccgccgcgat aatcgcggat gccgtatttc tgctgatacg
     2161 tgtaatcggc gtgccccgga cggaagctgt ccttgatctt cgagtagtcg ccgctgcgct
     2221 ggtcgacgtt ctcgatcagc aggccgatgg ccgtgcctgt cgtacggccc tcgaacacgc
     2281 ccgagaggat cttcacttga tcgggctctt gtcgctgcgt gacgtgccgc gaacgtcccg
     2341 ggcgacggcg atcgaggtcc ttttggatgt cggcttcggt cagcccgagg cccggcgggc
     2401 agccgtcgac gacgcagccc agtgcggggc cgtgactctc gccgaacgtc gtgaccgtga
     2461 acaatcgccc gtaggtatcg cctgccattg tgttcctgct ccagggcggc ccgggctcgc
     2521 atgcccgtgc gccgcgcgcc aagtgggcca atgatgcgcg cttagctcgc gtgaaggaat
     2581 tgccggagct cggctgccgt cgtgactagc acgccgtcgc caccgcgctc gaacgcgaga
     2641 gcgatcagcg gcaaacgcgc attggccgcc gcgaaacgct ccgcgccggc gccgagctcg
     2701 agaaaaagca cgccgtccgc agtcagccgg tcgagcgcac cgcgaaggat gcgctcggct
     2761 gcggcaaagc cagtcgggcc gctcgcgagc ccgagctcgg gctcgtgccg atactcggcc
     2821 ggtagcgcgg cgacttcggc ttcgggcacg tacggcggat tcgacacgat gacgcgatag
     2881 cgctcggcgg ccggcggaaa caggtcggcc tcgaacagcc ggacgcgcga gccgagcccg
     2941 tgctgcgcga cgttgcgcgc ggccacctcg agcgctgcgg ccgagatgtc cgtcgcgtcg
     3001 accgtgaccc cggcgcaata gtgagccgcg gcgatcgcga tgcagccgct gcccgtgccg
     3061 atgtcgagaa cgcggtcacc gtcgcgaagc gccgtccacg gcgcgaactc gcgctcgagg
     3121 acctcggcga gcggcgagcg cggcacgagg acgcgctcgt cgacgaagaa cttgaggccg
     3181 gcgaaccacg cttcgttgat cagatacgcg agcggctggc gctcgtcgac gcgccgctcg
     3241 gcgatcgcga gcgctcgcgg cacgagctcg gccgccggcg gcgcaccgaa gtcgacgtcg
     3301 cgccagtcct gcaggtgccg cagcagccag aacgcttcgt cggcggcatt gtccgtgccg
     3361 tgaccgaaca cgagccggtg cgcgtcgaag tagtcggcga gcacggcgat ccacgcgtca
     3421 cagtcgcgcg ccgctcgcag ccgtcgttcc aggtcgcccg cgctcatgcg gcgcgcatga
     3481 taacccgtcg ccaattgctc cctgcattgc tttacagccg gcgtccctag gcaatgcctc
     3541 cggcggacgt ccatgcccgc gcgcaggcgt gcgataatcc ccgcgtgctg ggtggaatgc
     3601 tgcgaattct cgtcgtcgct ttggtcgtgc tcgtcgcggc catgttccta ttgccgcgcg
     3661 cacagcgcgg cgaagcaccg caaaacgcga cggtcctacc ggagccgagg gcgctcgccg
     3721 atgttcgctt cgtcgacaag accggccgcg aagcacactt gagcgatttc aaaggcgact
     3781 tcacgttgct gttcttcggc ttcacgaatt gccccgacgt ttgcccgttg acgctgagcc
     3841 tgctcaagga agtgcgagcc gatgtggcga aacgcgctcc gcggctgacg ccgcgcgtcc
     3901 tgttcgtcag cgtcgacccg aagcgcgaca ctccggaacg catcgcggcg tacttgaacg
     3961 gcttcgatcc cgagttcctc ggcgtgacgg cttccgacga ggagctcgca ccgttgctgc
     4021 aaacgctcgg cgtcgccgtc gagaagcacg accacggcgg cgctgactac aacgtcgtgc
     4081 acaactcggc catttacgtg ctcgacccga acgcggactg gatcgccgtc tcgacgggac
     4141 cgcacgaccc gcaggtcgtg gcctccgatt atctgcgcat ccgccagcgg cgctcgggcg
     4201 gcccggcctc gcccggcgca tgacgtcgcg cgcctgggtc tggctgtcgc aacacctgat
     4261 cccgaaaaac ctctcgtcgg ctgtggtcta tcgagccacg cggtcgcggc gcgcgtggct
     4321 caggcgaccg ttgacgcgtt ggttcgcgaa gacctacggc gtcgacctga cggaagccgc
     4381 gaacgccgac ctcgacagtt acgcgacgtt caacgagttc ttcacgcgcg agctcaaagc
     4441 cggcgcgcgg cccatcgcct ccgaggcggg aacattggtc gcgccggcgg acggcgtgct
     4501 gacggagcac ggcgccgtcg acggcggccg cgtctatcag gctaagggca gcgcttacac
     4561 gctgcgcgag ctgctcgccg agagcggcgc cgccgtcagc cagctcgagg gcggccgcta
     4621 cttcacgatc tacttggcgc cgcacaacta ccaccgcgtg catgcgccgc tcgcggcgcg
     4681 cttgacgcgc acgcgctata ttcccggcgc gcgcttcagc gtcagccgag ccacggccgc
     4741 gacgatcccc aagatctttt gccgcaatga gcgcgtcgtc tgctggttcg agaccgacca
     4801 cgggccgatg gccgtcgtgc tcgtcggcgc actcaacgtc tcgagcatca gcaccttcgc
     4861 acacggcgag atttcgagcg gcgcgccgcg cgaatggcat gaaccgcaac cgcgtcccgt
     4921 cgagcgaggc ggagaaatcg ggcgcttcaa catggggtcg acggtcgtcg tgctgctcgg
     4981 ctcgagcgcg ttcgagttcg tcggcacggc ttgcgacggt ctcgccgtgc gcatggggca
     5041 agcgctcggc gcgttcacgc gggcttgatg ccgtgagcta cgacttaggc gcgtcgctcg
     5101 ccacgctgcg gcaccgcgct gcgatgctgg ccgcgatccg ctcgttcttc gccgcgcgcg
     5161 cggtgctcga ggtcgagacg ccggcgctgt cgccggcagg cgtcacggac cccgcgatcg
     5221 agagcatcgt cgccgaggcc cgctgcctcg ttccggcgcg acactacctg caaacgtcgc
     5281 ccgagttcgc gatgaagcgc ttgctagccg acggcagcgg cgacatctac cagctgtgcc
     5341 gcgtctatcg cgacgacgag ctcggccggt ggcatcagcc cgagttcacg ctgctcgagt
     5401 ggtaccgcgt cggttgggac gagcagcgcc tgatgacgga ggtcgcggag ctcatcgcgg
     5461 ccgcgtgcgc ggccgcgggc agtgcgttcc gatcgacggt gcgtttgacc tatgcgcagg
     5521 cgctcgaggc cgcgctcggc gtgggccccg aagcgccggc cggggagctc gcgcgcaggt
     5581 tggcgggcaa aggcgtcgac gtgccgccgg ggctcgcgca cgacgccgtg ctcgacctcg
     5641 cgttcggcac cgtcgtgccg gccagcttcg acgctcgcac tctgaccttc gtctacgact
     5701 atcctgcgag ccaagcggcc ttggcgcggc tcaagctcac gacgccgcgt gtcgcggcgc
     5761 ggttcgaagc gttcagcgct ggaatcgagc ttgccaacgg cttccacgag ctcgccgacg
     5821 ccgcggagca acgccggcgt ttcgtcgcgg aacgggagcg gcgcgtcgcg aacggccaac
     5881 acgcgccgcc gctcgacgag cagctactcg aggcgctcgc caagctgccg gattgcgccg
     5941 gcgtcgcgct cggcttcgat cgactcgtgg cgctcgcgac gggtcatgac aacgtggcgg
     6001 ccgtaatgag cttcgctcac ggccgcgatt cgtagtcagc tactgcttga cccgcgagat
     6061 gtaggtgccg gagcgagtgt cgactttgat gacctcgcct tcgttgatga acagcggcac
     6121 gcgtacggtc gcgcccgtct cgagcttcgc gggcttctga ccgcccgtcg cggtgtcgcc
     6181 gcggactccg ggatccgttt cgacgatctt gaggtcgacg tgattcggag tggcgatgta
     6241 cagcggcaca ccatcccaca acgtcacgat gaccatttcc tgctctttca tccacggctt
     6301 ggcatcggcc accgccgctt cgccggcggc gatctgctcg aacgtgtcgg gcaccatgaa
     6361 gtaccagaac tggccgtccg tgtagaggta ttgcatgtcg gtgtcgacga cgtcggcgcc
     6421 ttcggccgtg tcgccggact tgaaggtgcg ctcgacgacg cggcccgtct tcaggttgcg
     6481 gatcttgacc ctgttgaagg cctgcccttt gccgggcttc acgtactcgt tctcgagaat
     6541 ggcgtacgga tcgccatcga gcaggatctt gagcccgctc ttgaattcgt tggtgctgta
     6601 aactgccatg cgttggtcat caaaaaacag agcgcgggat cataccctta agctctacgg
     6661 aa
//
LOCUS       DATLEE010000491         3676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6990435, whole genome shotgun sequence.
ACCESSION   DATLEE010000491 DATLEE010000000
VERSION     DATLEE010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6990435"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..415
                     /locus_tag="VM692_10600"
     CDS             77..415
                     /locus_tag="VM692_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HUQ52663.1"
                     /translation="MKLITAVVKPFRLDDVRNALAEVGVQGMTVTEVKGFGRQRGHTE
                     LYRGAEYVVDFLPKVKVEVAVTDELVERVIEAIIGAAKTGKVGDGKIFVTELTAVYRI
                     RTGETGDQAI"
     gene            436..1728
                     /locus_tag="VM692_10605"
     CDS             436..1728
                     /locus_tag="VM692_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HUQ52664.1"
                     /translation="MKRILSASVALAAVLCPLLAGAQTIDTGSTAWMLTSTALVLLMT
                     LPGLALFYGGLVRTRNVLSVLMQCFALCCVISLIWVLFGYSLAFGEGNGWIGGLSKLW
                     MRTVEVGSTHSLAPAIPETVFSMFQLTFAIITPALIVGGFAERTRFAAMLLFSILWLI
                     VVYFPVAHWVWAPDGWLFMRGFRDFAGGAVVHVNAGIASLVAALVIGNRSGFPTTAMP
                     PHNLTMTVSGASLLWVGWFGFNAGSAVASGGPAGMAMLVTHIGAAAGATAWMSLEWFR
                     FGKPSVLGIVTGMVAGLGTITPASGFVGPMGAVVIGLVAGVICFFATQFIKRKLKIDD
                     SLDVFPVHGVGGFTGLLLTAVFSATAFDGLGLPEGVSIGQQLLTQLIGAVATVVWSGV
                     ASFVILKLVNLVSPLRVTTEQENEGLDLVLHEERGYSL"
     gene            complement(1730..3073)
                     /locus_tag="VM692_10610"
     CDS             complement(1730..3073)
                     /locus_tag="VM692_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ52665.1"
                     /translation="MSLFWKIFISFMIAMTVTSVGAIYVTYWIVSRPLTQTDFQGRPE
                     IISEVSAALARGGEYGLKSWLNDNPRPAPGIVLLVTNERGDELLGRAMPRELRALLRT
                     KTPFRLPNRPANFQPVQLTPTITGPDGAVYELRFETAPITIFGILVWPGTQVAVLALA
                     IFAAFVVSLALARYVSSPIVRLQKASRALAAGALETRVGTPFTRRRDEVGTLARDFDT
                     MAERIQELVTAKETLLRDVSHEFRSPLARIRMALALAERRAGEASRPDLARIEREAER
                     LDALVGQVMTLTRLRTTDAPPSDIVRLDAVIGEVVDDARFEYPDAHVEYAPKREVTLR
                     GDADGLKSAIENVVRNALTYGDRTKPIVVRLDVAADTATVRVIDSGPGVPEPELKRIF
                     EPFYRTDNSRDHRQDGQGIGLAITARVTELHGGTVTAANRAEGGLEIALELPLAA"
     gene            complement(3078..>3676)
                     /locus_tag="VM692_10615"
     CDS             complement(3078..>3676)
                     /locus_tag="VM692_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUQ52666.1"
                     /translation="SGIQGLRSAQRENYDLIILDVMLPGLSGFDVLKQLREAGSKTPV
                     LMLTARGDDVDRIVGLDLGADDYLPKPFNPRELLARVKAILRRTTEPEGDEPAELVVG
                     DLRVNLRRREAWLGEQPLKLTNAEFVILVTLMRSPGDVVSREALTRTALGRQLLPDDR
                     SLDTHISNLRRKLGSDADADVLIRSIRGSGYVLVPPEQ"
BASE COUNT          591 a         1156 c         1251 g          678 t
ORIGIN      
        1 aacaatacac aacagtagac ccaagacagg tcgcattctc gaagcaagcg gactttcaac
       61 gtttttaggg ggactcatga agctgataac ggcagtcgtc aaaccctttc gtctcgatga
      121 cgttcgcaac gcattggccg aggtcggcgt acagggtatg accgtcacgg aggtcaaagg
      181 gttcggccgc caacggggcc acaccgagct ctatcgcggc gcggaatacg tcgtcgattt
      241 cttgccaaaa gtaaaggttg aggtcgccgt tacggatgag ctcgtcgagc gcgtcatcga
      301 agcgatcatc ggggccgcga agaccggcaa ggtcggtgac ggcaagatat tcgtgaccga
      361 gctgacggcg gtctaccgaa ttcgcaccgg cgaaaccggc gatcaagcga tctaagaaaa
      421 cccggggggg ttcaaatgaa aagaatattg agtgcttcgg tcgcgctcgc ggccgtcctg
      481 tgcccgctgt tggcgggtgc gcagaccatc gacactggct ccaccgcgtg gatgctgacg
      541 tcgacggcat tggtgttgct gatgacgttg ccggggctcg cgctgttcta cggcggcctc
      601 gtccgaacgc gcaacgtgct ctcggtgctg atgcagtgct tcgcgttgtg ctgcgtcatc
      661 tcgctgatct gggtgctgtt cggctactcg cttgcgttcg gcgaaggtaa cggctggatc
      721 ggcggtctat cgaagctatg gatgcggacg gtcgaggtgg gcagcacgca tagcctcgct
      781 ccggcgattc ccgagactgt cttctcgatg ttccagctca cgttcgcgat catcacgccg
      841 gcgctgatcg taggcgggtt cgcagagcgg acgcggttcg ccgccatgct gctgttctcg
      901 attctctggc tcatcgtcgt ctacttcccg gtggcgcatt gggtgtgggc gcccgacggt
      961 tggttgttca tgcgcggctt ccgcgacttc gcgggcggcg ccgtcgtgca cgtcaacgcg
     1021 ggtatcgcgt ccctcgtcgc ggcgctcgtc atcggcaacc gtagcggctt cccgacgacg
     1081 gccatgccac cgcacaacct gacgatgacg gtcagcggcg cgtcgctgct ctgggtcggt
     1141 tggttcggct tcaacgcggg tagcgcggtc gcgtcgggcg gacccgccgg catggccatg
     1201 ctcgtgacgc acatcggcgc cgcggcaggc gcgacggcgt ggatgagcct cgagtggttc
     1261 aggttcggca agccgagcgt gctcggcatc gttacgggca tggtcgcggg cttgggcacg
     1321 atcacgccgg catcgggctt cgtcggcccg atgggtgcgg tcgtcatcgg tctcgtggcc
     1381 ggcgtcatct gcttcttcgc gacgcagttc atcaagcgca agctgaagat cgacgactct
     1441 ctcgacgtgt ttccggtcca cggcgtcggc ggcttcacgg ggctgttgtt gacggcggtg
     1501 ttctccgcga ccgcattcga cgggctcggt ctgcccgaag gcgtctcgat cggccaacag
     1561 ctgctgacgc agctgatcgg cgccgtcgcg acggtcgtct ggagcggcgt cgcgtcgttc
     1621 gtaatcctga agctcgtgaa cttggtctcg ccgctgcgcg ttacgacgga gcaggagaac
     1681 gaaggcctag acctcgtgct gcacgaggag cggggctaca gcctctgagt cacgcggcca
     1741 gggggagctc gagagcgatc tcgagccctc cctcggcccg attcgccgcc gtcactgtgc
     1801 cgccgtgcag ctccgtgacg cgtgccgtga tcgcgaggcc gatgccttgg ccgtcttgcc
     1861 ggtggtcgcg gctgttatcg gtgcgataga acggctcgaa aatccgcttg agctcgggtt
     1921 ccggaacgcc gggcccgcta tcgatgacgc gaaccgttgc ggtgtcggcg gcgacgtcga
     1981 gccgtacgac aatcggtttg gtgcgatcgc catacgtcaa cgcgttgcgc acgacgttct
     2041 cgatcgcgct cttcaagccg tcggcgtcgc cgcgcagcgt aacttcgcgc ttcggcgcgt
     2101 actcgacgtg agcgtcggga tattcgaatc gcgcgtcgtc gacgacttcg ccgatcaccg
     2161 cgtcgagccg cacgatgtcg ctaggcggcg catcggtcgt ccgcaagcga gtcaacgtca
     2221 tgacctgacc gaccaacgcg tcgaggcgct cggcttcgcg ttcgatgcgc gcgagatcgg
     2281 gccgcgacgc ctcaccggcg cgccgctccg cgagcgccaa ggccatgcgg atgcgtgcga
     2341 gcggcgaccg gaactcgtgc gacacgtcgc gcaacagcgt ttccttcgcc gtcaccagct
     2401 cctgaatgcg ctcggccatc gtgtcgaagt cacgcgcgag cgtcccgacc tcgtcgcggc
     2461 ggcgcgtgaa cggggtgccg acgcgagtct cgagggcgcc ggcggccagt gcacggctgg
     2521 ccttttgcag ccgcacgatt ggcgacgata cgtagcgcgc gagcgcgagt gacactacga
     2581 acgcggcgaa gatcgccagt gctagcacgg cgacttgcgt gccgggccac acgaggatgc
     2641 cgaagatcgt aatgggagcg gtctcgaatc tgagctcata cacggcgccg tcggggcctg
     2701 tgatcgtcgg cgtcaactgc actggctgaa aattggccgg acggttaggg agacgaaagg
     2761 gcgtcttagt tcgaagaagc gcacgcagct cgcgcggcat ggcgcggccg agcagctcgt
     2821 cgccgcgctc gttcgtcacg agcagcacga tgcccggtgc cggccgcgga ttgtcgttca
     2881 gccacgactt gagcccgtac tcgccgccgc gcgcgagcgc cgccgaaact tcgctgatga
     2941 tctcggggcg gccttggaaa tccgtctgcg tcagcggccg gctgacgatc cagtacgtta
     3001 cgtagatcgc gccgaccgac gtcacggtca tcgcgatcat gaacgagatg aagattttcc
     3061 agaacaggct cacggcgtca ttgctcgggc ggcaccagca cgtagccgct gccgcggatg
     3121 ctgcggatca acacgtcggc gtcggcgtcg ctgccgagct tgcgccgcag attgctgatg
     3181 tgcgtatcga gactgcggtc gtcgggcaac agctgccgac cgagtgccgt gcgggtcaac
     3241 gcctcgcgcg acacgacgtc gccgggcgag cgcatcagcg tcacgaggat gacgaactct
     3301 gcgttcgtga gcttcagcgg ctgctcgccg agccacgctt cgcgccggcg cagattgacg
     3361 cgcaggtcgc cgacgacgag ctcggcaggc tcgtcgcctt cgggctccgt agtgcggcgc
     3421 aagatggctt ttacgcgcgc gagcagctcg cgcggattga acggcttcgg caggtaatcg
     3481 tcggcgccga gatcgaggcc gacgattcgg tcgacgtcgt cgccgcgcgc cgtcagcatc
     3541 agcacgggcg tcttcgagcc ggcctcgcgc agctgcttca gcacgtcgaa gcccgacagg
     3601 ccgggcagca tcacgtcgag gatgatgagg tcgtagttct cccgctgcgc gctgcgcaac
     3661 ccctggatgc cggacg
//
LOCUS       DATLEE010000492         5450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6240806, whole genome shotgun sequence.
ACCESSION   DATLEE010000492 DATLEE010000000
VERSION     DATLEE010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6240806"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..467)
                     /locus_tag="VM692_10620"
     CDS             complement(72..467)
                     /locus_tag="VM692_10620"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HUQ52667.1"
                     /translation="MRDSLLFVYGTLRPCVDIRMSRWLRRNARYLGGATTAGRLYDLG
                     PYPAMRAAGVCHDRVIGDVYRVVQRRVLSVLDRYEAGEVRRRPRFVRERCIVKLARGA
                     NRTAWTYRYRYSVAGAPRIVGGDYREHRR"
     gene            528..1922
                     /locus_tag="VM692_10625"
     CDS             528..1922
                     /locus_tag="VM692_10625"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HUQ52668.1"
                     /translation="MRLCVLLLALFAATVQARGVSPYLPVNLSPEIERKIERVLILAE
                     QPLLKRPIAAATVLDALPRACERDRSLCEDVRRYLTSLTRTAGISYGSVSIGGGTGAD
                     TPLPNRHGMSSQSNYEAAAALYWQPGDHLLVTAGALAYDGDTNPTGSVVSIGSEYLQI
                     DVGYRDRWWSPSLDNAMLLSTQAQTLPSVTISNYTPFTRAKLRYEAFLGELSESANIA
                     FGAGTTTGKPRIAGLQLSVEPLEGWSIGVSRIMQYGGGERDDSLRDLFDAFFNPSERD
                     NTGPASTGDFGNQAASFTSQFVLGDPLPIAVYFEYAGEDTSRSNNFRLGNSALSAGIT
                     FPQLGANFAATFEVTEWQNGWYAHHIYRDGLRNDGRVLGHWGGDWRALVDEVGAHSLF
                     ARVTWTPAFGTLEASYRQLDNEQYGNRIYETARQIDARYSRPWDQVFVGAELTLGRDV
                     FGESYSRLGAFIRF"
     gene            1934..2467
                     /locus_tag="VM692_10630"
     CDS             1934..2467
                     /locus_tag="VM692_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52669.1"
                     /translation="MRQPLFASITAITAALSVANARADAFVDIGIHASHLEADVATFA
                     RVTTTETGVHLGAGLRRDLARGSIGARLEFDNVDGDLLLAVRAFDWSRNLSDRFAVTA
                     FAGAARLDLATPAYGYYYGAGMRLKELVAGWDLGIDLRYGDKLARDNLLPTDPQGGKN
                     DNFYDLSSVAVYLSRRF"
     gene            complement(2446..3516)
                     /locus_tag="VM692_10635"
     CDS             complement(2446..3516)
                     /locus_tag="VM692_10635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUQ52670.1"
                     /translation="MTVVLYRPSLDARSGAGQLLEMQWRGLVAAGVPALLGCERGALR
                     FWLRTGVRAHRRSAQQLERLRERGELVMDHGLALPSAEIVFVHNLASEAQRHVPHAGS
                     AQAVQHEQQYFRALNPSATVVANSTLVAEALHAHFALARERIHVLYPGFRSQRYSQRR
                     AVELRVAARRAFGLAPQTPLVGLITSGDFGKRGLDLFLESAARIASARPDVRFLVVGS
                     KELPEHARANALVRGGVVLYRPKGRRPEASFAALDLFLYPARFEEYGMVVAEAQAMGV
                     PIVTSRLVGASECLPEAYAPWIVERPEPAVLAENALALLADTELRRGLASVAATTVAA
                     FDERAYVEGTLRLLALQNRRLR"
     gene            complement(3541..4755)
                     /locus_tag="VM692_10640"
     CDS             complement(3541..4755)
                     /locus_tag="VM692_10640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ52671.1"
                     /translation="MPVITHLVSLQQAAGVEAHFSELVRQARASDPKFSHGWLNAAGP
                     MHAFVADRVAGQLAHCIDAKRWNGLRLPAKPRALRTWHCRRALAAAHTDVLVIWNRTA
                     KAQFAVDAIGERRCIHWEHGAAWDAGRDAERRAYLQRVPLAIANSTASARVLQLLWDY
                     RGEVRVCRNALRPSLVPAAPKAKRFPRERIKLGAAARLYPVKGLALLLHGVAALHRLR
                     PSLDVELHVAGEGPELTRLRALAAELGVADRVTFHGVVSDMGAFYERIDCLVHAPLTE
                     AFGLVAIEAAAHGCPVVTAHIDGLAEAVAVGVTGRTIEPTLPLERYVALGGRLEGLPQ
                     CIYDPASDALAEPRAVDPEQLAAAVAELFDDAAAYEQLSACASAHVLAQPTFAAHVRD
                     VLAVIDGFIRRS"
     gene            complement(4798..4873)
                     /locus_tag="VM692_10645"
     tRNA            complement(4798..4873)
                     /locus_tag="VM692_10645"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4838..4840),aa:Lys,seq:ctt)
     gene            complement(4887..4962)
                     /locus_tag="VM692_10650"
     tRNA            complement(4887..4962)
                     /locus_tag="VM692_10650"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4927..4929),aa:His,seq:gtg)
     gene            complement(4993..5068)
                     /locus_tag="VM692_10655"
     tRNA            complement(4993..5068)
                     /locus_tag="VM692_10655"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5033..5035),aa:Arg,seq:tct)
     gene            complement(5091..5167)
                     /locus_tag="VM692_10660"
     tRNA            complement(5091..5167)
                     /locus_tag="VM692_10660"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5131..5133),aa:Pro,seq:tgg)
BASE COUNT          977 a         1841 c         1767 g          865 t
ORIGIN      
        1 gacaggggcg cggcgaattg cgcgtcacgt aatgcaacgt cgcgattgcg cgaccttcga
       61 cataacgcgt gctagcgacg atgctcgcgg taatcgccgc cgacgatgcg cggcgcgccg
      121 gcgacgctgt aacgatagcg atatgtccac gccgttctgt tcgcgccgcg cgccaacttc
      181 acgatgcagc gctcacggac gaaccgcggt ctgcgacgca cctcgccggc ttcgtagcga
      241 tcgagcacgc tcaacactcg tcgctgcacg actcgataca cgtcgccgat gacgcggtca
      301 tggcagacgc cagccgcacg catagcggga tacggcccga ggtcgtagag ccgacccgcg
      361 gtggtcgcac cgcccaaata gcgagcgttg cgtcgcaacc agcgcgacat ccgaatgtcg
      421 acgcagggtc gcaacgttcc atagacgaac agcaacgaat cgcgcactgc gagccttgac
      481 gatatcatgc tggcctcgcg cttcatggta acggccggga gattgtcgtg cgactctgcg
      541 ttttgctgct ggcgttgttt gcggcgaccg ttcaggcgcg cggcgtcagt ccctacctgc
      601 ccgtcaattt gtcgccggag atcgagcgca agatcgagcg cgtgctgatc ctcgccgagc
      661 agccgctgct gaagcgtccg atcgcggccg cgaccgtgct cgatgcgctg ccgcgtgcgt
      721 gcgagcgcga ccgctcgctc tgcgaggacg tgcgccgcta tctgacgagt ctcacgcgca
      781 cggccggcat cagctatgga tcagtgagta tcggcggcgg cacgggtgca gacaccccgc
      841 tgccgaatcg tcacggcatg agctcgcaaa gcaactacga ggccgccgcg gctctctact
      901 ggcaacctgg agatcacttg ctcgtgaccg caggagcgct ggcgtacgac ggcgacacga
      961 acccgacggg cagcgtcgtg agcatcggca gcgagtatct gcaaatcgac gtcggctatc
     1021 gcgaccggtg gtggtcgccg tcgctcgaca acgcaatgct gctcagcacg caggcgcaga
     1081 cgttgccctc ggtgaccatc tccaactaca cgccgttcac gcgtgcgaag ctacgttacg
     1141 aagcgtttct cggcgagctc agcgagtcgg cgaacattgc gttcggcgcc ggtacgacta
     1201 cgggcaaacc gcgcatagca ggcctgcaac tctccgtcga gccgctcgag ggttggtcga
     1261 tcggcgtgag ccgcatcatg caatacggcg gcggcgagcg tgacgattcg ctgcgcgacc
     1321 tcttcgacgc gttcttcaac ccgtcggagc gtgacaacac gggccccgca tcgacgggcg
     1381 acttcggcaa tcaagccgcg tcgttcacgt cgcagttcgt gctcggcgat ccactgccga
     1441 tcgcggtcta cttcgagtac gccggcgagg acacgtcgag gtcgaacaac tttcggctcg
     1501 gcaattcggc cttgtcggcc ggcatcacgt ttccgcagct cggagcgaat tttgcggcga
     1561 cgtttgaagt tacggagtgg cagaacggtt ggtacgctca ccacatctac cgcgacggcc
     1621 tccgcaacga cggccgcgtg cttggccatt ggggcggcga ctggcgagcg cttgtcgacg
     1681 aggtcggcgc gcacagcctg ttcgcgcgcg tgacgtggac cccggcattc ggcacgctcg
     1741 aagcctcgta tcggcagctc gataacgagc agtacggcaa ccgtatatat gagacggcgc
     1801 gacaaattga tgcgcgctac agccggccat gggaccaggt gttcgtgggc gccgagctca
     1861 cgctcggccg cgacgtgttc ggcgagtcgt actcgcgcct cggcgcattc atccggttct
     1921 agacgagcgt tacatgagac agccattgtt cgcgagcatc acggcaatca ccgccgccct
     1981 ttcggtcgcg aatgcacgcg ccgacgcctt cgtcgacatc ggaatccacg cctcgcacct
     2041 cgaggccgac gtcgcgacgt tcgcgcgagt cacgaccacg gagacgggcg tgcacctcgg
     2101 cgcgggcctg cgacgcgacc tggcgcgtgg cagcatcggc gcgcgcctcg agttcgacaa
     2161 cgtcgacggc gacttgctgc tggccgtgcg cgcgttcgac tggagcagaa acctgtccga
     2221 tcgatttgcc gtgacggcat tcgccggagc agcgcgtctc gatcttgcaa cgccggccta
     2281 cggctactac tacggcgccg gcatgcggct caaggagctg gtggcgggct gggatctcgg
     2341 catcgacctg cgctatggcg acaagctcgc acgcgacaac ttgttgccga cggatccgca
     2401 aggcggcaag aacgacaact tctacgatct atccagcgtc gcggtctacc tgagccgccg
     2461 gttctgaagc gcgagtaagc gcagcgtccc ttcgacgtac gcgcgctcgt cgaatgccgc
     2521 gacggtcgtc gccgccacag acgcgagccc acgacgcagc tccgtgtccg cgagcaacgc
     2581 cagcgcgttt tccgccagca cggcgggttc cggtcgctcg acgatccacg gcgcatacgc
     2641 ctccggcaag cattcgctag cgccgacgag ccgcgaagtc acgatcggca cacccatcgc
     2701 ctgcgcttcg gcgacgacca tgccgtattc ctcgaaccgg gccgggtaca agaacaggtc
     2761 gagggccgcg aacgaagctt ccggccggcg gcccttcggc cgatacagca cgacgccgcc
     2821 gcgcacgagc gcattggccc gcgcgtgctc gggcaattcc ttcgaaccga cgacgaggaa
     2881 tcgaacgtcc ggccgcgcgc tcgcgattcg cgccgccgac tcgaggaaca ggtcgagccc
     2941 acgcttgccg aagtcgcccg acgtgatcag cccgacgagc ggcgtttgcg gcgcaagccc
     3001 aaacgctcgc cgcgcagcga cgcgcaactc gacggcgcgc cgttgtgagt agcgctgcga
     3061 tcgaaagccg ggatagagaa cgtggatgcg ctcacgtgcg agagcgaagt gggcgtgcaa
     3121 cgcttccgca acgagcgtcg aattagcaac gacggttgcc gacggattca gcgcgcggaa
     3181 gtactgctgt tcgtgctgca ccgcttgcgc cgagcccgcg tgcggcacgt ggcgctgcgc
     3241 ttcgctggcg agattgtgca cgaagacgat ctcggcgctc ggcagagcaa gcccgtgatc
     3301 catgacgagc tcgcctcgtt cgcgcaaccg ctcgagctgc tgcgcggaac ggcgatgcgc
     3361 acgcacgccc gtgcgaagcc agaagcgcag cgcgccgcgc tcgcagccga gcagcgccgg
     3421 aacgcccgct gcgacgagac cgcgccattg catctccagt aactgtccgg cgccgctgcg
     3481 agcgtcgagg ctcggacgat agagcacgac tgtcatgcga cgacgccgct ccgcacgcag
     3541 ctaggagcgg cgaatgaagc catcgatgac tgccagcacg tcgcgaacgt gcgcggcgaa
     3601 cgtcggttgt gcgagcacgt gcgcgctcgc gcatgcgctc aactgctcgt acgcggccgc
     3661 gtcgtcgaac agctcggcga cggcagcagc caactgctcg gggtcgacgg cgcgcggctc
     3721 ggcgagcgcg tccgaggccg gatcgtagat gcattgcggc agaccctcga gcctgccgcc
     3781 gagcgcgacg tagcgctcga gcggcaacgt cggctcgatc gttcgcccag tgacgccgac
     3841 ggccacggcc tcggccaagc cgtcgatgtg cgccgtcacc acgggacagc cgtgcgcggc
     3901 ggcctcgatc gcgacgagcc cgaacgcttc ggtgagcggc gcgtgcacga ggcaatcgat
     3961 gcgctcgtag aaagcgccca tgtcgctgac gacgccgtga aacgtcacgc ggtcggcaac
     4021 gccgagttcc gccgccaacg cacgcaaacg cgtcagctcg ggcccttcgc cggccacgtg
     4081 cagctccacg tcgagcgacg gacgcagccg gtgcagcgct gcgacgccat gcaaaagaag
     4141 cgcaaggcct ttcacgggat acaagcgcgc cgcggccccg agtttgatgc gttctcgcgg
     4201 aaatcgtttt gccttgggcg cggccgggac gaggctgggc cgcaacgcgt tgcggcagac
     4261 gcgcacctcg ccgcggtagt cccagagaag ctgtaggacg cgcgccgacg ccgtcgagtt
     4321 cgcgatcgcg agcggcacgc gctgcaaata ggcgcggcgc tccgcgtcgc gcccggcgtc
     4381 ccatgccgcg ccgtgttccc aatgtatgca acgacgctcg ccgatcgcgt cgacggcgaa
     4441 ctgcgctttg gcggttcgat tccagatgac gagcacgtcg gtgtgcgcgg cagcgagcgc
     4501 gcgtcgacag tgccacgtgc gcaaggcgcg cggcttggcg ggcaagcgca atccgttcca
     4561 acgcttcgcg tcgatgcaat gcgccagctg accggcgacg cggtccgcga cgaaggcatg
     4621 catcggcccc gccgcattga gccaaccgtg cgagaacttc ggatcgctgg cacgcgcctg
     4681 ccgcacgagc tcgctgaagt gagcttcgac tccggcagcc tgctgcaagc tcacgaggtg
     4741 cgtgattacc ggcacgcgct gccgctcatc gcgaaacgac cgcaggcggg catgaggtgg
     4801 tgggccgtgt aggactcgaa cctacaacca atggattaag agtccactgc tctaccaatt
     4861 gagctaacgg cccggctacg ggtaattggg gtggacgatg ggactcgaac ccacgacggc
     4921 cggaatcaca atccggggct ctaccaactg agctacgtcc accagcgtgt tgtcattcta
     4981 tcgcatgagt cctggcgcgc ccggcaggat tcgaacctgc aacccccggc ttagaaggcc
     5041 ggtgctctat cccttgagct acgggcgcaa tcttcagcaa ggtttgttct tggtcggggc
     5101 agcaggattt gaacctgcga ccctctgctc ccaaagcaga tgcgctacca gactgcgcca
     5161 tgccccgttc gcttttcagc gtggcatctt atgcttccta gccttgagca agcaaaccag
     5221 cggccggcgc tcggccgcga attgcttctg cattcgcggc acgccgccat ccatggcggc
     5281 gccttgagcc gccaaagaag ctcgtgcgag aatccgcgtc cccgcgcagc taggaatcca
     5341 agtgaccgcc acgctcctcg acggtaaagc cgttgcccag caaatccgtg acgagctcac
     5401 gacccgggtc actgacgttc aagagaagct cggacgcccg ccggcgctcg
//
LOCUS       DATLEE010000493        12553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2798103, whole genome shotgun sequence.
ACCESSION   DATLEE010000493 DATLEE010000000
VERSION     DATLEE010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 12553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12553
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2798103"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VM692_10665"
     CDS             <1..379
                     /locus_tag="VM692_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52672.1"
                     /translation="GAKSASSWRVQFAAAAVVVLALGAVLAAVLLRVERPAEVPLAAE
                     APQAQPLVASAFQPLLNAPGLSPSLSYNVVRVRIPLSALALVPGTQEGGTIEADLLVG
                     EDGLARGIRFAGADTMLVSVAAQ"
     gene            394..1470
                     /locus_tag="VM692_10670"
     CDS             394..1470
                     /locus_tag="VM692_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52673.1"
                     /translation="MSSKHGLTSLVASVCGAWATLASAQGPEPLPPLPPEDTLFFLQN
                     APPLPALRGRVDVIRGEGSVMGPVVEGKPYSARSITESTQVLADGNRIMQRNESAFYR
                     DSAGRTRREQTLGGVGPWQTGEPVTIINIHDPVADKTYMLDPVGRIAREIKPFTMAFA
                     HTQQLEGAEEARAALERAEVQLRTVYSEAVPAPAPVPGGGARGVVTVIRGSEGVVGSS
                     TAFAPIAAGVDINAEIGAYEPGEDLGEQVLEGLLVHGTRMKDTIPAGAMGNERPIEIV
                     TERWFSKDIDAMVLERFSDPRVGETVYRLVNVVRGDPSPALFEVPQDYSLAADEGPRG
                     GVRVGAGGAVQWERRIEPGTPAPR"
     gene            complement(1479..2075)
                     /locus_tag="VM692_10675"
     CDS             complement(1479..2075)
                     /locus_tag="VM692_10675"
                     /inference="COORDINATES: protein motif:HMM:NF028494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HUQ52674.1"
                     /translation="MPATLAGVYAGELPCSNCAAIEATLWLRPDGRFFLRQSFRDDAT
                     GFAAAQAPSRTYGLGRWSWDEAAAETVLRGAGPDRRLTVVDEQHLELRTAPAGQHVLA
                     RDAAAPPFDDRLTLDGESSVSENGATFTECLTGLKWAVADAGAYRELRRQHRRLNPRG
                     KIAVTTIEGHLVTTHKGATTSERLVVDRFITMKPGQGC"
     gene            2323..3072
                     /locus_tag="VM692_10680"
     CDS             2323..3072
                     /locus_tag="VM692_10680"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HUQ52675.1"
                     /translation="MNARVPPVLHRASPRFFVLFLALTVWAFWPSYFSRLFEQPSVLH
                     HAHGIVLASWLVMLVAQAQLIRTQRRSLHRQLGKLSYVLAPAVVVVTTAFVHQRMAPG
                     LAGYATLPVPALLSLALMLGSLVVFAVFYGFAIAKRRDSQTHARWMVCTVFPMFTPVT
                     DRLIAVHWQTLIGVLPRIDGNPVLPVAGFALADVLLVALSWWDWRANRRLDVFPVALG
                     VLLVYHVATLTLHLVPAWNAFGAWFLSLPLS"
     gene            complement(3074..3727)
                     /locus_tag="VM692_10685"
     CDS             complement(3074..3727)
                     /locus_tag="VM692_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HUQ52676.1"
                     /translation="MRSTALALLVAVASSASAHAQGQAVATPPFEAGKHYTVLTPPQP
                     TSSDAGKVEVAEVFMFGCPGCYGLEPHLQEWLGRKADYIEFVRIPAPWNGPAVVHARA
                     YYTAEALGKAEAIDADFFNEIHMNRNLLETEEKLADFFAKHGVDAATFTNTFNSFAVD
                     AKVKRADELIQRYRAGSTPTVVVNGKYTTMGRQAGSYEAWFAIIDDLAAREHAAAAK"
     gene            3934..4524
                     /locus_tag="VM692_10690"
     CDS             3934..4524
                     /locus_tag="VM692_10690"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HUQ52677.1"
                     /translation="MKKNLLALLSLGVMAGFAASAVADDDHDGHGRQQRVFRAKLIGF
                     NEVPSVSSAAEGQFYAVVNKEGTSFTYWLSYSGLAFDAAQSHIHFGQHHVNGGISVWL
                     CQGTVPAPAAVAAQTPTCATRATTTPITGTIDASDVIGPAGQGISATEFAELLAAMRG
                     GAAYANVHSGVAGNPTAMPPTPTVGFPGGEIRGQVN"
     gene            4696..5937
                     /locus_tag="VM692_10695"
     CDS             4696..5937
                     /locus_tag="VM692_10695"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52678.1"
                     /translation="MATVRAVSLAAAVLCATNAFAHHGIANFDLNKDIAISGTVSKLA
                     FVNPHSWLYVNVVGADGKTAEWQCEMRAATVLRRSGWSEAMFTQGTAVSITGSPDRNA
                     PNTCYLGTITFADGTSMDRYGQRQKTEVAVAANRPERLPNGDPNINGDWAGEQRVMTD
                     RRGISGALVPISVADTLAPGQVPDGGRAFPGARGTPESLSQDAIRAAWTRPSPLPLTD
                     AGRQAAEGFDPSSDDNPRLRCEPTNILFDWGFETFTNRITQDENSIRIQYGAPGIDRT
                     VDLNETAHPASIEPSVAGHSIGHWENDVLIVDTVGFKPGVLSADAYMMHSAGLHIVER
                     FSLDRSTGALRREYVAEDPAYFVGQYRGADTVFPADLPYETPSCNDLSYKTGEGTAAI
                     EQPTTAPAAVPASKPWWMFWK"
     gene            5980..6675
                     /gene="lepB"
                     /locus_tag="VM692_10700"
     CDS             5980..6675
                     /gene="lepB"
                     /locus_tag="VM692_10700"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014509427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HUQ52679.1"
                     /translation="MSEPDSKTSAEPAAVSKAQSSRAKWWISELISLAIVLAAVTAAR
                     SSLADHYFVPSGSMEYSLMPGDRVIVNKMSYGVRIPLTKIDIFGSSTPERGEIAVFDS
                     PQDGTTRLIKRIVAIGGDRVSVVDGQLSINGQALGDRMVEHFGKRDALLNLTSQPFVN
                     HGGGPNFEKVVPPGMVLAMGDNRGNSNDGRAFGFIDERELFGRAVAVYYRSSNCTWYD
                     LPCVFSGFTWKPL"
     gene            complement(6715..8331)
                     /locus_tag="VM692_10705"
     CDS             complement(6715..8331)
                     /locus_tag="VM692_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HUQ52680.1"
                     /translation="MSCPSNLTHSMYADGELSARESTLLELHAATCAACGARIEALRR
                     ERAVLRLALREVEDEAPIPRFVPPPRPRDFVVLVLAVVLIGGFSSAFWNTVSAAVPDA
                     LRWLNPFESGELFERAVSVVTFIVYEGSAMWTAALNFIGAALIVAFVAWLSSGAVRRR
                     GFAAVAVSLLAVVIALPSTGHALEVRHEKNLITVPAGETIDDSVIAAATKITIDGNIN
                     GDLLAFGQEVTVRGNVTGNLVTGAQRVTIEGTIGGTVIGGGENVSFARARVGRDLYGF
                     GSDVDIASDAEVSGNATAFGKTVAVNGRVGRDLHSFGEAVRISGNVGDDVVAGGAHIA
                     LLPTARVTGDVSARVDSADDLEIDSAAVVGGNVDKQVVEREQRRNHYATFGYYFSQVV
                     RLGAMFLAGLALLWVFPVLRSVLLRDSVAVVRSFGIGLAAAVTLPVAAIMLCLTVVGI
                     PIGILTMFLGAVALYFSKIVVAQIIGSAVLSAPQEPRHVAATLVTGLVIVIVAINLPW
                     IGGIANLIMTLVGLGLIVTLLFERFNRAPA"
     gene            complement(8328..8873)
                     /locus_tag="VM692_10710"
     CDS             complement(8328..8873)
                     /locus_tag="VM692_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUQ52681.1"
                     /translation="MKEAAFADAYTAHFRQVRGLCRQLLGSTDRGEDAAQEAFIRAYR
                     ALASYDPAQPFGAWIMRIARNHCLDLLRRRSTEKALFGSEADEAALAEAPDLGGLATV
                     LSVERASAVNAAVAKLPERYRVPLALAYYADADYNEIASQLGLTRTHVGVLLCRAKHL
                     LRESLAEAGMTTHGAVAEVSQ"
     gene            8983..9369
                     /locus_tag="VM692_10715"
     CDS             8983..9369
                     /locus_tag="VM692_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52682.1"
                     /translation="MAEAEAAWSLAAGLARLTDGLEQLADVIEAGNKAEARTAGNLAA
                     SYADRFYGRVRDKLQRDAQVPEPELEHCFKVVLAFDQVQSALPASAAELKIGVVEALI
                     DRYYEGHPPEKKRAALQQLTELKRAR"
     gene            complement(9553..11151)
                     /locus_tag="VM692_10720"
     CDS             complement(9553..11151)
                     /locus_tag="VM692_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52683.1"
                     /translation="MSPKLLYDRLSLGPVVGRHQPTLHLAALLIVFVLLDRAVERVTG
                     LSESSYFQTSLVIELARKLGWLGCVSLAAAAVLLTRYGKLGSAWGALECGAALRTFVV
                     VLIGALAWCLSTYGYNYYFDQGYYVERVLLVALVPLTWWRPVFALPFLALAYTFLWQL
                     AEPALGGSIFPHKLQILRAVSLFAAAFLVYAVTGYRRIAPLLVLLCSFVAAAYWVAAV
                     AKYNLSWLSDARLLTMPVSAYAHGWLAFLDPATVVAVSTAIGRYDPVLRALVLTLEGA
                     CLFFLWRRSLSMWLLGLLILFHCAIFALYGFFFWTWMLLDGALMLLLWRAGGSADTGV
                     YNVRNAVLSVGLILAGSWWARPPQLGWFDTPLTYTYRIQALDAEGRRSTVHPYFLTPY
                     EDVFTMTAFSYLATDHPRLVGAYGATADADLARELFDARTPADVFAIENEREREPDRA
                     RAARFYRFLEQYFATRNRRGERLCYLRYLRPPQQFWSFRGDWPDVEHDRVAQLAVVEV
                     TTFFNGKGLEVIRESELARIKIPH"
     gene            complement(11141..>12553)
                     /locus_tag="VM692_10725"
     CDS             complement(11141..>12553)
                     /locus_tag="VM692_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HUQ52684.1"
                     /translation="DLPIQHWFFERIHIDPQHWNQSLLLRVATRVDRVLFERAVQRLM
                     NHHDALRARFVPVAGAVRQEIAPVVVEPPVDWVEIADSTEEEILRHIEQRADRAHRAF
                     ELDRGPLVRFLYFSIPSALGDRLLVLAHHLVVDVVSWQILIEDLESMLAALEGTAGVA
                     QPERTTSLGRWAARLAEYAGTAEVAAQAEHWLGLDSEVEVPTDFSADKTENTVETAFS
                     VSLDLDVAETSRLLTEVPKALRSQANEALLAALATTLTRWTGRSVCRIDLEGHGREPL
                     FDDVDLSRTVGWLTTVFPVELKVGGDRSAAAAVRAIQAQLRAIPARGIGYGLLRYLSP
                     TASSGLSGLPRSLVCFNYLGRIDALGSHGRRLSPVLWNVGTVRSPTGRRPYLIELDVH
                     VEAGRLIAVWTASQRFHRRETIAGLAEQFRAMLRDIVAAAETGSVTATPDDFPLADLD
                     AGDLESLSRMIETSERQREP"
BASE COUNT         2265 a         4107 c         4111 g         2070 t
ORIGIN      
        1 gggcgcaaag agcgcgagct cgtggcgcgt tcagttcgca gcggcggccg tcgtcgtgtt
       61 ggccctcggt gcggttctcg cggcggtgct gttgcgcgtc gagcgcccgg cggaagtgcc
      121 gctcgcggcc gaggcgccgc aggcgcagcc gctcgtcgcc agcgcgtttc agccgttgct
      181 gaacgcaccg ggcttgtcgc cgtcgctgtc gtacaacgtc gtgcgcgtgc gcatcccgct
      241 ttctgcgctc gcgctcgtgc cgggcacgca agagggcggc acgatcgagg ccgatttgtt
      301 ggtgggcgag gacggcctcg cgcgcggaat tcgtttcgcc ggggccgaca cgatgctcgt
      361 gtcggtcgcc gcgcaatgac ctggagaatc gcaatgagct cgaaacatgg actgacttct
      421 ctcgtcgcga gtgtctgcgg tgcctgggcg acgctcgcga gcgcgcaggg gcccgagccg
      481 ctaccgccgc tgccaccgga ggacacgttg ttcttcttgc agaacgcgcc gccgttgccc
      541 gcgctccgtg gccgcgtcga cgtgatccgc ggcgagggca gcgtcatggg cccggtcgtc
      601 gaaggtaagc catacagcgc gcggtcgatc accgagtcga cgcaggtgct cgcggacggc
      661 aaccgcatca tgcaacgcaa cgagagtgcg ttctatcgcg actcggcggg gcgcacgcgg
      721 cgcgagcaga cgctcggtgg cgtcggccca tggcagaccg gcgagcccgt cacgatcatc
      781 aacattcacg atcccgtcgc cgacaagacc tacatgctcg accccgtcgg gcgcatcgcg
      841 cgcgagatca agccgttcac gatggcgttc gcgcatacgc aacaactcga aggcgccgag
      901 gaggccagag cggcgctcga acgcgcggag gtccagctgc gaacggtata cagcgaggcg
      961 gtgcccgcac ctgcgcctgt gcccggcggc ggcgctcgcg gcgtcgtgac cgtgatccgc
     1021 ggcagtgaag gcgtggtcgg gagcagcaca gcgttcgcgc cgatcgccgc gggcgtcgac
     1081 atcaatgccg agatcggcgc ttacgagccc ggcgaggatc tcggcgagca agtgctcgaa
     1141 ggcttgctcg tgcacggcac gcgcatgaag gacacgatcc cggccggcgc gatgggcaac
     1201 gagcggccga tcgagatcgt gacggaacgc tggttctcga aggacatcga cgcgatggtg
     1261 ctcgagcgct tctccgatcc gcgcgtcggc gagaccgtct accggctcgt gaacgtcgtg
     1321 cgcggcgacc cgtcgccggc gttgttcgaa gtgccacagg attactcgct ggcggccgat
     1381 gagggcccgc gtggcggagt cagagtgggg gcaggcggcg ccgttcagtg ggagcgacgc
     1441 atcgaacccg gtacgccggc acctcgctga cgctgcagtc agcagccctg accgggcttc
     1501 atcgtgatga atcgatcgac gacgagccgc tcgctcgtcg tcgctccctt gtgcgtcgtg
     1561 acgaggtggc cttcgatcgt cgtcaccgcg atcttgccgc gcggattcag gcgccggtgt
     1621 tgacggcgca gctcgcgata cgcgccggca tcggctaccg cccacttcaa tcccgtcaag
     1681 cattccgtga acgttgcgcc gttctcggat acggagctct cgccatcgag cgtcaagcga
     1741 tcgtcgaacg gcggcgccgc ggcgtcgcgc gccaagacat gctggccggc tggcgccgtg
     1801 cgcaactcta ggtgctgctc gtcgacaacc gtcagccgcc gatcggggcc cgcgccgcgc
     1861 agcacggtct ccgctgcagc ctcgtcccaa gaccagcggc ccagcccgta ggtccgcgac
     1921 ggtgcctgcg ccgccgcgaa gcccgtggcg tcgtcgcgga agctctgacg cagaaagaac
     1981 cgaccgtccg ggcgcagcca caacgtggct tcgatcgccg cgcaattgct gcacggtagc
     2041 tcgccggcgt agacgccggc gagcgtcgcg ggcatcggtg ttcgcgtttc gacgtctccg
     2101 ttggagccgc cgctgcagcc gcagagcaac gcggcgagcg cgagaatggc tggtgaaagg
     2161 cgagacatgc cgccatcttc cgccgctgcc gccgcgcgac tagtaaatgg atcacgttcg
     2221 catggccgtc tttgcgcctc tgtgaccgcc cgcgcagcgc ctggacccca tcgggcgcct
     2281 tcctttagag tctcccggcc tttgccgccg gagaaaccgc tcgtgaacgc ccgcgtgccg
     2341 cctgtcttgc accgcgcaag cccgcgcttc tttgtcctct ttttggcgtt gacggtctgg
     2401 gcgttctggc ccagctattt ctcacggctg ttcgagcagc cgagcgttct acaccacgcg
     2461 cacggcatcg tattggcgtc gtggctcgtc atgctcgtcg cgcaagcgca gctgatccgc
     2521 actcagcgtc gctcgttgca ccgccagctc ggcaagctgt cgtacgttct ggcgccggcc
     2581 gtcgtcgtcg tgaccacggc gttcgtgcat cagcgcatgg ctccggggct cgcggggtat
     2641 gcaacgctac cggtgcccgc gttgctttcg ttggcgctga tgctgggctc gctcgtggtg
     2701 ttcgcggtgt tctacggttt cgcgatagcg aagcgccgcg actcgcagac gcacgcgcgc
     2761 tggatggtgt gcacggtctt cccgatgttc acgcccgtga cagatcggct gatcgccgtg
     2821 cactggcaaa cgctgatcgg tgtgttgccg cgcatcgacg gcaaccccgt tctccccgtc
     2881 gcgggattcg cgctggccga cgtgttgctc gtcgcgctgt cgtggtggga ctggcgcgcg
     2941 aatcggcggc tcgatgtctt tcccgtcgcg ttaggcgtgc tgctggtcta tcacgtcgcg
     3001 acgttgacgc tgcatctcgt gccggcgtgg aacgcgttcg gcgcgtggtt cttgagcctg
     3061 ccgctgtcct agctcacttc gctgcggcgg cgtgctcgcg tgcagcgaga tcgtcgatga
     3121 tcgcgaacca cgcttcgtag ctgcccgcct gccggcccat cgtcgtgtac ttgccgttga
     3181 cgacgacggt cggcgtgctg ccggcgcgat agcgttggat gagctcgtct gcgcgtttca
     3241 cttttgcgtc gaccgcgaac gagttgaacg tgttcgtgaa cgttgccgcg tcgacaccgt
     3301 gcttcgcaaa aaagtccgca agcttctctt cggtctcgag caggttgcga ttcatgtgga
     3361 tctcgttgaa gaagtcggcg tcaatggcct cggccttacc gagcgcctcg gcggtgtaat
     3421 aagcgcgcgc gtgcacgacc gccggcccgt tccacggcgc cgggatcctc acgaattcga
     3481 tgtagtcggc tttccgccct agccattcct gcaaatgggg ctcgaggcca taacagcctg
     3541 ggcagccgaa catgaagacc tcggcgacct cgaccttgcc ggcatcgctg ctcgtgggct
     3601 gagggggagt caacacggtg tagtgcttgc cggcttcgaa cggcggcgtg gcgacggcct
     3661 gcccttgggc gtgcgccgac gccgaggacg ctacggcaac gagcagggcc agcgctgtcg
     3721 atctcacgtt cccactcctt gaatcagagg cttatcgccc ggttttcgcg caaaagctta
     3781 gccgccggtc gggccgggcg gcaacgcgcc gctgctcgtc ccgagaaatc cattgtttcg
     3841 tgcgcgtcat gaacctcgcg gtgcttggcg cggactcacg acgcccaaag tgtcttcacg
     3901 agggcgtctg ctcacacaaa accggagggg gagatgaaaa agaacttgct ggcattactg
     3961 tcgctcggcg tcatggcggg tttcgcggcg tcggccgtcg cggacgatga tcacgatggc
     4021 cacggcaggc agcagcgcgt gtttcgcgcg aagttgatcg gtttcaacga agtgccgtca
     4081 gtatcgagcg cggcggaagg ccagttctat gccgtcgtga ataaagaggg gacgtcgttc
     4141 acctactggc tttcgtattc gggtctcgcc ttcgacgctg cccaatcgca catccacttc
     4201 ggccagcatc atgtgaacgg cggtatcagc gtctggctgt gccaaggcac ggtacctgcg
     4261 ccggccgccg tggccgctca aacgccgacg tgtgcaacgc gagcgacgac gacgccgatc
     4321 acgggcacca tcgacgcgag cgacgtcatc ggcccggcag gccagggcat cagcgcgacg
     4381 gagttcgcgg agctgctggc tgccatgcgc ggcggagccg cgtacgcgaa tgtgcactcc
     4441 ggtgtagccg gtaacccgac cgccatgccg ccgacaccaa cggtggggtt ccccggcgga
     4501 gaaattcgcg gccaggtcaa ctagccggct gccgaccttt ccggtttgcc ccgtgggcgc
     4561 cccgcgcccg cggggctccg ccgcgcctgc agaacgttgc cgcagcgttc gcggcatgct
     4621 ggacgagccc gctatttgcc gagccggggg tcgaaatgtc acagtgcgag tcgaagaaag
     4681 cgaaggagct cgccgatggc gaccgtgcgt gctgtgtcgt tggccgccgc cgttttgtgc
     4741 gctacgaacg cgttcgcaca tcacggcata gccaatttcg acctgaacaa ggacatcgcg
     4801 atcagcggca cggtctcgaa actcgcattc gttaacccgc attcgtggct gtacgtcaac
     4861 gtcgtcggcg ccgatggcaa gacggccgag tggcagtgcg agatgcgcgc ggcgacggtg
     4921 ctgcgccgct ccggctggag cgaggcgatg ttcacgcaag gcacggccgt cagcatcacg
     4981 ggctcaccgg atcgtaacgc ccccaacacc tgctacttgg gaacgatcac gttcgccgac
     5041 ggcacgagca tggatcgcta tggccagcgc caaaaaaccg aggtggccgt cgccgcgaac
     5101 cggcccgagc gcctgccgaa cggggacccg aacatcaacg gcgattgggc cggtgagcag
     5161 cgcgtgatga ccgatcggcg cggcatcagc ggggcgctcg tgccaatcag cgtcgcggac
     5221 acgctcgcgc cgggtcaggt cccggacggc ggacgcgcat ttcccggtgc gcgcggcact
     5281 cccgagtcgt tgtcccaaga cgccatccgc gccgcctgga cgcggccgag cccgttgccg
     5341 ctcacggacg ccggacggca agcggccgaa ggcttcgatc cgtcgtcgga cgacaatccg
     5401 cggctgcgct gcgagccgac gaacatcttg ttcgactggg gattcgagac gttcacgaac
     5461 cgcatcacgc aggacgagaa ctcgatccgc atccagtacg gcgcgcccgg catcgatcgc
     5521 acggttgatc tgaacgagac ggcgcatccg gcgagcatcg agccgagcgt cgcagggcat
     5581 tcgatcggcc actgggagaa cgacgtgctg atcgtcgaca ccgtcggctt caagcccggg
     5641 gtgttgtcgg cggacgcgta catgatgcac agcgccggtc tgcacatcgt cgagcggttc
     5701 tcgttggatc gctcgacggg cgcgctgcgg cgcgagtatg tcgccgagga tccggcgtat
     5761 ttcgtcggcc agtatcgcgg cgccgacacc gtgttcccgg ccgacttgcc gtacgagacg
     5821 ccgagctgca acgacctcag ctacaagacc ggcgagggaa cggcggccat cgagcagccg
     5881 accacggcgc ctgcggcggt gcctgcctcg aagccctggt ggatgttctg gaagtgagcc
     5941 gctaggaacc agttcgcccc gcaacttgtc ggagttcccg tgtccgagcc agacagtaag
     6001 accagcgccg aaccagccgc cgtttctaaa gcccaatcga gccgtgccaa gtggtggatc
     6061 tccgagctga tctcgctggc gatcgtgctc gccgccgtta cggctgcgcg ttcgtcgctc
     6121 gccgatcact acttcgtccc gtcgggctcc atggaatact cgttgatgcc gggcgaccga
     6181 gtcatcgtca acaagatgtc gtacggcgtg cggattccgc tgacgaagat cgacatcttc
     6241 ggctcgtcga cgccggagcg cggtgagatc gccgtgttcg attcgccgca ggacggcacg
     6301 acgcggctca tcaagcgcat cgtcgcgatc ggcggcgacc gcgtatcggt cgtcgacggc
     6361 cagctctcga tcaacggtca ggcgctcggc gaccgcatgg tcgagcactt cggcaagcga
     6421 gacgcgctgc tgaacctcac gtcgcagcca ttcgtcaacc acggcggcgg accgaatttc
     6481 gagaaagtcg tgccgcccgg catggtgctt gcgatgggcg acaatcgcgg caacagcaac
     6541 gacggtcgcg ctttcggctt catcgacgag cgcgagctgt tcggtcgcgc cgtcgcggtc
     6601 tactaccgca gcagcaactg cacgtggtac gacctgccgt gcgtgttcag cggcttcacg
     6661 tggaagccgt tgtgacggca gccgcgcggc agtagtacgt cagatcgcgg gttgtcaggc
     6721 cggtgcgcgg ttgaagcgct cgaacagcag cgtcacgatg agcccgagcc cgaccaaagt
     6781 catgatgagg ttggcgatcc cgccgatcca cggcagattg atcgcgacga tgacgatcac
     6841 gagcccggtc accagcgtcg ccgccacgtg ccgcggctcc tgcggggcac tcaacaccgc
     6901 gctgccgatg atctgcgcga ccacgatctt cgaaaaatag agcgcgacgg cgccgagaaa
     6961 catcgtcagg atgccgatcg ggatccctac gaccgtgagg cacagcatga tcgccgcaac
     7021 cggcagcgtc acggccgccg cgaggccgat gccgaacgag cgcacgacgg cgacagagtc
     7081 gcgaagcaat acgctgcgca gcacggggaa tacccacagc agtgcgaggc ctgccaggaa
     7141 catcgcgccg agacgcacga cttggctgaa gtagtagccg aaggtcgcat agtggttgcg
     7201 gcgctgttcg cgctcgacga cttgcttgtc gacgttgccg ccgacgacgg cggccgagtc
     7261 gatctcgaga tcgtccgcgc tgtcgacgcg cgccgagacg tcgcccgtga cgcgggccgt
     7321 cggcagcagc gcgatgtgcg ccccgccggc gacgacatcg tcgccgacgt tgccgctgat
     7381 gcgcacggct tcgccgaagc tgtgcaggtc gcggcctacg cggccgttga ccgcgacggt
     7441 cttaccgaac gcagtcgcat tgcccgagac ttcggcgtcg gacgcgatgt cgacgtcact
     7501 gccgaaacca tagagatcgc ggccgactcg cgcacgtgcg aaggacacgt tttctccgcc
     7561 gccgatgacg gtgccgccga tcgtgccttc gatcgtgacc ctctgtgcgc ccgtcacgag
     7621 attgccggtg acgttgccgc gcaccgtgac ttcctggcca aacgcgagca ggtccccgtt
     7681 gatgttgccg tcgatggtga tcttcgtggc agccgcgatg acagagtcgt cgatcgtttc
     7741 gccggccggc accgtgatga ggttcttttc gtgccgcact tcgagcgcgt ggccggtcga
     7801 cggcaacgcg atcacgaccg cgagcagaga gaccgcgact gcggcaaatc cgcgtcgacg
     7861 cacggccccg gacgagagcc aagcgacgaa cgcgacgatc agcgcagcgc cgatgaagtt
     7921 cagagcagcg gtccacattg cagatccctc gtaaacgatg aaggtcacga cactgacggc
     7981 gcgctcgaag agctcgcccg attcaaatgg gttcaaccaa cgcagcgcgt cgggtaccgc
     8041 ggccgaaacc gtgttccaga acgcgctgct gaagccgccg atcagaacga ctgcgagcac
     8101 gagcacgacg aagtcgcgcg ggcgcggcgg tggcacgaag cgcggaatcg gcgcctcgtc
     8161 ttcgacttcc cgcagcgcga gtcggagcac ggcgcgttcg cggcgtagcg cttcgatgcg
     8221 cgcgccgcag gcagcgcagg ttgccgcgtg cagctcgagt agagtcgatt cgcgcgccga
     8281 cagctcgccg tcggcataca tcgaatgagt cagattggaa gggcagctca ttgggatacc
     8341 tcggcaactg ctccatgcgt tgtcatgccc gcctcggcga gcgattctcg taatagatgt
     8401 ttggcgcggc acagcagcac gccgacgtgc gtgcgtgtca ggccaagctg gcttgcgatc
     8461 tcgttgtaat cggcgtcggc gtagtaggcg agtgcgagcg gtactcgata gcgctcgggt
     8521 agcttcgcga cggcggcgtt gacggccgaa gcgcgttcga cgctgagcac ggtcgcgagg
     8581 ccgccgaggt ccggggcttc ggccagcgcc gcttcgtcag cctcggagcc gaacagcgcc
     8641 ttttccgtgc tgcgccggcg caggaggtcg agacaatggt tgcgcgcgat cctcatgatc
     8701 caagcgccga acggctgcgc cgggtcgtag ctcgccaacg cgcggtaagc gcggatgaac
     8761 gcttcctgcg ccgcatcctc gccgcggtcc gtcgagccga gcaactgccg gcacaacccc
     8821 cgcacctgac ggaaatgcgc tgtgtaagcg tcggcaaacg cggcctcctt catcatccct
     8881 tgtcctggct tcgcctctca gtgggtaata cgcgcgagcc ctcgaattca ttacagtaag
     8941 cgcatggcgg aatcacgtga gctaggcgcg gcgcgagttt gcatggccga ggccgaagcg
     9001 gcctggtcgt tggccgctgg cttggcccgg ctaaccgacg ggctcgagca gctagccgac
     9061 gtcatcgagg caggcaacaa ggccgaggcc agaaccgccg gcaacttggc agccagctac
     9121 gccgaccgct tctacggccg cgtgcgcgac aagctgcagc gcgacgctca ggtgccggag
     9181 cccgagctcg agcactgctt caaggtcgtg ctggcattcg accaggttca gagcgcgctg
     9241 ccggcatcgg cagccgagct caagatcggc gtcgtcgagg cgctgatcga tcgctactac
     9301 gaaggtcatc cgccggagaa gaagcgcgcc gcgctccagc aattgacgga gctcaagcgt
     9361 gcccgttgaa ggccgactga ggcgcgcgcg taacccaagc agactgccga cgcgacaatc
     9421 gccgcgaccc accgagtata ttgccggcta atgtgccgta tgccgccgca ttgagaaacg
     9481 gtgcgagtcc cagggcaagc cgaaacgggg ctagtgcgcg taagtcgcag gcaaaaattt
     9541 cgatccactt cgtcagtgcg ggatctttat ccgtgcgagc tcggactcac ggatgacttc
     9601 caaccccttg ccattgaaaa aagtcgtgac ttcgacgacg gcgagctggg cgactctatc
     9661 gtgctcgacg tccggccaat cgccgcgaaa tgaccagaac tgctgcggcg gacgcagata
     9721 ccgcaggtag cagaggcgct cgccgcgcct gttacgggtc gcgaaatatt gctccaagaa
     9781 gcgatagaaa cgcgcggcac gggcccgatc cggctcgcgc tcgcgttcgt tctcaatcgc
     9841 gaagacgtcc gcaggcgtgc gggcatcgaa caactcgcgc gcgagatcgg cgtcggcggt
     9901 cgctccatag gcaccgacga gccgcggatg gtccgtcgcc agatacgaga aagccgtcat
     9961 cgtaaacaca tcctcgtagg gcgtgaggaa gtagggatga acggtgctgc gccggccttc
    10021 tgcgtcgagg gcctgaatcc tgtaggtgta ggtcagcggc gtgtcgaacc atcccagttg
    10081 cggcggcctg gcccaccagc ttccggcgag aatcaacccg acggacagca ccgcgttgcg
    10141 cacgttgtaa acgccagtgt cagccgagcc gccagcgcgc cacagcaaca gcatcaaagc
    10201 gccgtccagc aacatccagg tccagaaaaa gaaaccgtag agggcgaaaa ttgcgcaatg
    10261 aaaaaggatc agcaagccta ggagccacat cgatagggag cgacgccaca gaaaaaacag
    10321 gcacgcgcct tcgagcgtca gcaccaacgc acgcagaacc gggtcgtacc gaccgatcgc
    10381 cgtggacacg gctacgacgg ttgccggatc gaggaacgcc agccaaccgt gtgcataggc
    10441 gctgacaggc atggttaaaa ggcgtgcgtc ggacagccac gacaggttgt acttggcgac
    10501 ggccgctacc caataggcag cggccacaaa cgaacaaagc agcaccagca gcggcgcgat
    10561 ccgccgatat ccagtaacgg cgtacacgag aaaggccgcg gcgaacaaac tgaccgctcg
    10621 cagaatctgc agcttgtgcg gaaagatcga tccccccaac gcgggctccg cgagctgcca
    10681 taaaaacgtg taggccaacg ctaagaaagg cagcgcaaac accggtcgcc accacgtcaa
    10741 cggcactaac gccaccaaga gaacacgctc gacgtaatag ccttggtcga agtaatagtt
    10801 gtatccatac gtcgagaggc accaggccaa ggcgccgatc aagaccacga caaaggtacg
    10861 cagggccgca ccgcactcca gcgcccccca tgccgagcca agcttgccgt agcgcgtcaa
    10921 caggaccgcc gcggcggcta gactcacaca cccgagccaa cccagcttgc gcgctagctc
    10981 gatgacgagc gacgtttgga agtagctgga ttccgacagg cccgtcacgc gttcaacggc
    11041 cctgtccaac agcacgaaaa cgatcaggag cgccgccagg tgaagggtcg gctggtggcg
    11101 gccgacgacc gggccgagcg acagccggtc atacagcaat ttagggctca cgctggcgct
    11161 cggaggtctc gatcattcgc gacaagctct cgagatctcc ggcatcgagg tcggcaagcg
    11221 gaaagtcatc gggcgtcgcc gtgacgctgc ccgtttccgc cgcagcgaca atgtcgcgga
    11281 gcatcgcccg aaactgctcc gctaggccgg cgatcgtctc gcgccggtga aagcgctgac
    11341 tggcagtcca aaccgcgatc agacgaccgg cttcgacgtg cacgtcgagc tcgatgaggt
    11401 aaggccttct gccggtcggg ctgcgtacgg tgccgacgtt ccaaagcacc ggcgagagac
    11461 gccggccgtg gctgccgagc gcatcgatac ggccaagata attgaagcac acgagcgatc
    11521 gaggcaatcc cgagagtccc gagctagccg taggcgatag atagcgcagc agcccatagc
    11581 cgatgccccg cgccggaatc gctcgcaact gcgcctggat cgccctcaca gcggcagcgg
    11641 ccgagcggtc cccaccgacc ttgagctcca ctgggaaaac cgtggtcagc cacccgaccg
    11701 ttcgcgacaa atccacgtca tcgaacaatg gttcgcgccc atggccttcg agatcgatgc
    11761 ggcacacgct gcgcccggtc caacgcgtca acgtagtcgc caatgccgcg agcagggcct
    11821 cgttggcctg ggaacggagc gcctttggca cctccgtgag caagcgggac gtctccgcga
    11881 catcgagatc cagcgacacg gagaaggccg tctcgaccgt gttttcagtc ttgtctgccg
    11941 aaaaatccgt gggaacttcg acttcgctgt cgaggcccag ccagtgctcg gcctgtgccg
    12001 caacctccgc agtgcccgcg tactccgcca gtcgggccgc ccaccgcccg agcgacgtcg
    12061 tcctttccgg ttgtgcgaca ccggcagtgc cttcgagcgc agccaacatg ctttcgagat
    12121 cctcgatgag gatttgccag gacacgacgt caacgacgag gtgatgcgcc agcaccagca
    12181 accgatcgcc gagtgcgctc ggtatcgaga agtataggaa tcggaccagc ggtccacggt
    12241 ccaactcgaa ggctcgatgg gcgcgatcgg cgcgttgctc gatgtgccgc aggatttcct
    12301 cttcggtaga gtctgcgatc tccacccagt cgacgggcgg ctcgacgacc accggcgcga
    12361 tctcctgccg aacggccccg gccaccggta cgaatcgcgc cctcaacgca tcgtgatgat
    12421 tcatcaggcg ctgcacggca cgctcgaaca gcacacgatc gacgcgcgtc gcgacgcgta
    12481 gcaacaggga ttggttccaa tgttgtggat ctatgtggat acgttcgaaa aaccaatgtt
    12541 ggatgggaag atc
//
LOCUS       DATLEE010000494         2782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6991823, whole genome shotgun sequence.
ACCESSION   DATLEE010000494 DATLEE010000000
VERSION     DATLEE010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6991823"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="VM692_10730"
     CDS             <1..1594
                     /locus_tag="VM692_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HUQ52685.1"
                     /translation="PGDGAAMMFRLADVYFAPGEIGNELDVVAIDGLEAVARMEQEVL
                     SLSGPGATLNLDVGAVADFEFSQAFGPLAETALLTRWNNIDGIFSEQLGVQINVATTQ
                     IFTTSNDPFTTSAASALLDELANYRGATTAQDAQGITHLFTGRDLDGSTAGIAYFGAV
                     CAHRGSFDGRSFGAGLSEARRGAVTDSLVAAHEIGHLFGAPHDGESTGACSATPTTFL
                     MAPNINGSDQFSACSIRQIQAEIATARCLTPIGPANVKVLLTQPAQNALAGASFSQTA
                     TVRNDGADLATNTTFAATTEQGLEIVAVDAGGAACTVAANAANCSLGTLSGGTSRTVT
                     LTLRAQSPGTLDLTATVAADADDVATDNSDTVTVTAIPAVDLVVSGTAPGIALNAQAT
                     INAVLDNAADFAATAVVVSAVASAGLRADQATLAGTACTIAAQAITCPARALAARANV
                     TLVVTATGIVAGSQQLTVAATADEAERAPANNQLAIAVSVSAPQQGGGGGALSWWVVA
                     ALLASQCLAANGRRRQRVPTPS"
     gene            1647..1997
                     /locus_tag="VM692_10735"
     CDS             1647..1997
                     /locus_tag="VM692_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52686.1"
                     /translation="MTAAVERAVNCLAASIEPAVDAIAARIEPLGRDIVAASLGALRG
                     SIQTVIDTIALAIEPLLGAITAVVEPPLDAIAAGIRALGRRLRQRLHGARQQPQTEPY
                     CTAFHLRLPSQQCG"
     gene            2067..>2782
                     /locus_tag="VM692_10740"
     CDS             2067..>2782
                     /locus_tag="VM692_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUQ52687.1"
                     /translation="MDGKIKGRGALSNQQGRFARYERDPDLEALAAAAAAEEDVAPAK
                     LATNLHVDRARTIIARNNSPDISFSQSINPYRGCEHGCIYCYARATHSYLDLSPGRDF
                     ETEIFYKPNAVELLRAELAAPRYAVSPIAFGTNTDPYQPVERKLAVMRGLLELLLELR
                     HPLTIVTKGALILRDLDLLHALAQLNLVAVHVSVTTLDNELKRRMEPRTAGPQQRLDM
                     IRQLADAGVPTGVMAAPMIPA"
BASE COUNT          471 a          979 c          908 g          424 t
ORIGIN      
        1 tccgggcgac ggcgccgcca tgatgttccg tttggccgat gtttacttcg cgcccggcga
       61 gatcggtaac gagctcgacg tcgtcgcgat cgacggcctc gaagccgtcg caagaatgga
      121 gcaggaagtt ctgtcgctgt cggggccagg cgcaaccctg aatctcgacg tcggcgccgt
      181 cgccgatttc gagttctcgc aagcattcgg tccgttggcc gaaaccgcgt tgctgacgcg
      241 ctggaacaac atcgacggca tcttcagcga gcagctcggt gtgcagatca acgtcgccac
      301 gacgcaaatc ttcacgacga gcaacgatcc gttcacgacg tcggctgcca gcgcgttgct
      361 cgacgagctc gcgaattatc gcggcgcgac taccgcgcag gacgcgcagg gaatcactca
      421 cctattcacg ggccgtgacc tcgacggctc gacggccggc atcgcgtact tcggcgccgt
      481 ttgtgctcac cgcggctcgt tcgacgggcg cagcttcggc gccggcttga gcgaggcgcg
      541 ccgcggcgcc gtcacggaca gcctcgtggc ggcgcatgag atcggccact tgttcggcgc
      601 gccgcacgac ggcgaatcga cgggagcgtg cagcgctacg ccgacgacgt tcctgatggc
      661 gccgaacatc aacggctccg accagttctc tgcgtgcagc atccggcaaa tacaggccga
      721 gatcgcgacg gcccgctgtc tcacgccgat cggccccgcc aacgtcaagg tgcttctgac
      781 gcaaccggca cagaacgcgc tcgcgggcgc ttcgttcagt cagacggcga ccgtcaggaa
      841 cgacggcgcg gacttagcga cgaacaccac gtttgcggcg acgaccgagc aaggcttgga
      901 gatcgtggcc gtggacgccg gcggcgcagc ctgcaccgtc gcggcgaatg ccgcgaattg
      961 ctcgctgggc acgctgagcg gcggcacgtc gcgcaccgtg acgttgacgt tgcgcgcgca
     1021 gagccccggc actctcgacc tgacggcgac cgtcgcggct gacgccgacg acgtcgcaac
     1081 cgacaacagc gatacggtga cggtgaccgc catcccggcc gtcgatctcg tcgtatcggg
     1141 caccgcgccc gggatcgcgc tcaacgcgca ggcgacgatc aacgcggtgc tcgacaacgc
     1201 cgccgacttc gcggcgacgg ccgtcgtcgt gtcggcagta gcgagcgcag gcttgcgcgc
     1261 cgaccaagcg acgctggccg gcacggcctg cacgatcgcg gcgcaagcga tcacgtgccc
     1321 cgcgcgcgcg ttggccgcgc gggccaacgt cacactcgtc gtgacggcca cgggcatcgt
     1381 ggccggcagt cagcaattga cggtcgccgc gaccgccgat gaagcggaac gcgcgcccgc
     1441 gaacaaccag ctcgcaatcg cggtcagcgt cagtgcgccg caacagggag gcggcggggg
     1501 cgcgttgtcc tggtgggtcg tcgcggcgtt gctggcatcg caatgcctcg ccgcgaacgg
     1561 acggcgccgg cagcgcgtgc cgacgccgtc ctgaagcgca atccgcggcg ccgttcgccg
     1621 cgccgatcca ggcgacgatc gatgcgttga ccgcggcggt cgagcgtgcg gtcaattgtc
     1681 tcgccgcgtc gatcgagccg gcggtcgacg cgatcgccgc gcgcatcgag cctctcggcc
     1741 gcgacatcgt ggccgcgtcg ctcggcgcgc tccgaggctc gatccagacg gttatcgaca
     1801 cgatcgccct tgcgatcgag ccgctcctcg gcgcgatcac cgcggttgtc gagccgcccc
     1861 tcgatgcgat cgccgcgggc atccgcgcgc tcggccggcg actgcgccaa cgcctgcatg
     1921 gcgcacgcca gcaaccccaa actgagccat actgcactgc gtttcatctt cgcctcccta
     1981 gccaacaatg cgggtagtcg ctgctaacaa cgggcctccc cgggcggcgg ttgacggcgc
     2041 accatcgcag ccaccgggct tatgtaatgg acggcaaaat caaaggtcgt ggcgctttat
     2101 cgaaccagca aggccggttc gcgcgctacg aacgcgatcc cgatctcgag gctctcgctg
     2161 ccgcggcagc ggccgaagaa gacgtcgcgc ccgcaaagct ggcgacgaat ctgcacgtcg
     2221 atcgcgcccg caccatcatc gcgcgtaaca actcgcccga catctcgttc agtcagtcga
     2281 tcaatccata tcgcggctgc gagcacggct gcatctactg ctacgcgcga gcgacgcact
     2341 cgtacttgga tctttcgcca ggccgcgact tcgagacgga aatcttctac aagccgaacg
     2401 ccgtcgagct gctgcgcgcg gagctcgcgg cgccgcgcta cgccgtaagc cccatcgcgt
     2461 tcggaacgaa tacggacccg tatcagcccg tcgaacgcaa gctcgcggtg atgcgcggct
     2521 tgctcgagct gttgctcgag ttacgccatc cgttgacgat cgtgacgaag ggcgcgctga
     2581 ttctgcgcga cctcgactta ctgcacgcgc tcgcgcagct gaatctcgtc gccgtgcacg
     2641 tcagcgtcac gacgctcgac aacgagctca agcgccgcat ggagccgcgc acggcggggc
     2701 cgcaacagcg gctcgacatg atccggcaac tggcagacgc cggggtcccg accggcgtca
     2761 tggccgcgcc gatgattccg gc
//
LOCUS       DATLEE010000495         3749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2052919, whole genome shotgun sequence.
ACCESSION   DATLEE010000495 DATLEE010000000
VERSION     DATLEE010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052919"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /gene="dcd"
                     /locus_tag="VM692_10745"
     CDS             <1..458
                     /gene="dcd"
                     /locus_tag="VM692_10745"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HUQ52688.1"
                     /translation="YGTSSYGYDVRCSDEFKVFTNVYSTVVDPKNFDEKSFVDVHADV
                     CIIPPNSFALARTVEYFRIPRSTLVVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFS
                     NTTTLPAKIYANEGVAQMIFFESDEVCETSYADRGGKYQGQRGVTLPRT"
     gene            complement(470..1198)
                     /locus_tag="VM692_10750"
     CDS             complement(470..1198)
                     /locus_tag="VM692_10750"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HUQ52689.1"
                     /translation="MIRWSMLAVLALAAHTAYGADASVATYAATYRVIYKGKDAGTAE
                     FNVRYLADQDIYEFRSSVMAKGMLKLARPNPAVERSQFRVDAAGVRPQGFWYEDGSRS
                     GEDNFHIDFDWQRNVATVSNAEARREFAVPEAVQDRGSLQVALMRDLAATGAVKSFKL
                     ADEDGVIEYEYAENGTATMPVGKGPAVATRILTQQRVGSSRITSLWVAPSLRFLPVRM
                     EQRRDGEVQTAFELISVTGLSGAQ"
     gene            complement(1195..1857)
                     /gene="purN"
                     /locus_tag="VM692_10755"
     CDS             complement(1195..1857)
                     /gene="purN"
                     /locus_tag="VM692_10755"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005320176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HUQ52690.1"
                     /translation="MTLKTTRVAVLISGEGSNLQALIDAAGAGRLGAEIVAVVSNRSA
                     ARGLERARTAGIAALHLGAVRGQERAEYDAALAALLAPYAPELLVLAGFMRILGPAFI
                     ERFAGRMLNIHPSLLPKYPGLDTHRRVLEAQDQWHGATVHFVTQELDAGPAILQYRVR
                     VRAGDTTESLAQRVHVGEHIILPRAVSWFAAGRLRLGAGSVMLDGRALQGPVSLEEGE
                     SQ"
     gene            1885..2997
                     /locus_tag="VM692_10760"
     CDS             1885..2997
                     /locus_tag="VM692_10760"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HUQ52691.1"
                     /translation="MAAIISDLVSQWALSMGLLSLLRGSIVVALAVVAAPSGAATFAD
                     LYTVKIAPDPAATDREKTATQAAMARLLIRVTGSRNAPLEPALQPLIAAPEDFLTTRG
                     SDREGRSIVGFRRGQVERVLTERGMRVWGPERPLTLLWIAVDDGAGGRALLGANEAAQ
                     FGEPATPQMTQLLATIREEVVAAADERGLPIAWPLLDLQDLNAVTFIDVSGRFDDRIV
                     AASARYRADAVLIGNVRPGILGNEVDWLFVNGTERQALPVTAVRDGLDAAADRYAAEL
                     STVGGASVAQLTVRDVRTPADYGRVMSYLERQSVLESVDVESYGDGTLSLRVAARGDA
                     RVLERVLALGGVLRPAGGGGAPGSLTFDVVGAGSAP"
     gene            2994..3554
                     /locus_tag="VM692_10765"
     CDS             2994..3554
                     /locus_tag="VM692_10765"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUQ52692.1"
                     /translation="MSLRFVPNAICIARMLLVAPLVSWIVHGRFAAALLVLVVAGLSD
                     GLDGYLAKRFDWRTRLGGLLDPAADKLLLVSTFAALSYVGLIPVELTIVVIGRDVVIV
                     LGAICYQWFIAPVQGEPAAISKLNTACQLGMLFFTLTNAAFAWPPPVSLLVLGAAVVF
                     TSIVSGLTYVLRWSARAWRVAHGATA"
     gene            3563..>3749
                     /locus_tag="VM692_10770"
     CDS             3563..>3749
                     /locus_tag="VM692_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="controls initiation of DNA replication by
                     inhibiting re-initiation of replication, promotes
                     hydrolysis of DnaA-bound ATP; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HUQ52693.1"
                     /translation="MGQLPLALALADYASFATFVSGSNVAAVEHVRSLAAGRGDTVWL
                     FGAAGAGKSHLLQAACRA"
BASE COUNT          559 a         1239 c         1296 g          655 t
ORIGIN      
        1 cctatggaac ctcgagctac ggctacgacg tgcgctgctc ggatgaattc aaggtattca
       61 ccaacgttta cagcaccgtg gtcgatccga agaacttcga cgagaagagt ttcgtcgacg
      121 tgcacgcgga cgtctgcatc atcccgccga attccttcgc gctcgcgcgc acggtggaat
      181 atttccgcat cccgcgatcg actctcgtcg tgtgcctcgg aaagtccacg tacgcacgct
      241 gcggcatcat cgtgaacgtg acaccgctcg agccggagtg ggaagggcac gtgacgctcg
      301 agttctcgaa cacgacgacg ttgccggcga agatctacgc gaacgagggc gtcgcacaga
      361 tgatcttctt cgagtcggac gaagtttgcg agacgagcta cgcggatcgc ggcggcaaat
      421 accagggcca gcgcggcgtc acgctgccac ggacctgagc cacagtcggc tactgcgcgc
      481 cgctcaagcc cgtgacgctg atgagctcga aggccgtctg cacttcaccg tcgcgccgct
      541 gctccatgcg cacgggcaag aatcgcaggc tgggcgcaac ccatagcgag gtgatgcgcg
      601 acgagccgac acgctgctgc gtcaggatgc gcgttgcgac ggcgggtccc ttgccgacgg
      661 gcatcgtcgc cgtgccgttc tccgcgtact cgtattcgat gacgccgtct tcgtcggcga
      721 gcttaaagct tttgacggcg cccgttgccg cgaggtcgcg catgagcgct acttgtaggc
      781 tgccgcgatc ttgcaccgct tccggtactg cgaactcgcg ccgtgcttcg gcgttgctga
      841 ccgtcgcgac gttgcgctgc cagtcgaagt cgatgtggaa gttgtcctcg ccggagcggc
      901 tgccgtcctc gtaccagaag ccttgcggtc gtacgcccgc ggcgtcgacg cgaaactggc
      961 tgcgctcgac ggcggggttc gggcgagcga gcttcagcat gcctttcgcc atcacgctcg
     1021 agcggaactc gtagatgtct tggtcggcga gatagcggac gttgaactca gccgtcccgg
     1081 cgtctttgcc tttataaatg acgcggtacg ttgccgcata cgtcgcgacg cttgcgtcgg
     1141 cgccgtacgc cgtgtgtgcc gccagagcga gtacggcgag catcgaccat cggatcattg
     1201 gctctcacct tcttcgagcg acacgggccc ttgcagcgcc cgcccatcca gcatgacgga
     1261 gccggccccg agcctcaagc gcccggccgc aaaccaagac acggcgcggg gcaggatgat
     1321 gtgctcgccg acgtggacgc gctgcgccaa ggattccgtc gtatcgccgg cgcggacgcg
     1381 cacgcggtat tgcaggatcg ccggccccgc gtcgagctcc tgcgtcacga agtgcaccgt
     1441 tgcgccgtgc cattgatcct gcgcctcgag cacgcggcgg tgcgtgtcga ggcccggata
     1501 cttcggcagc aacgacggat gaatgttcag catgcggccg gcaaaccgct cgatgaaagc
     1561 cgggcccaaa atgcgcatga accccgccag cacgagcagc tccggcgcgt acggagcgag
     1621 caatgccgca agcgccgcgt cgtactcggc gcgctcctgc ccgcgcacgg cgccgagatg
     1681 cagcgctgcg atgccggccg tgcgtgcgcg ctcgaggccg cgtgccgcgc tgcggttgct
     1741 gacgacggcg acgatctcgg cgccgagccg gccagcgccg gccgcgtcga tcaaagcttg
     1801 tagattgctg ccttcgccgg agatcaagac ggctacgcgc gtggtcttca gtgtcatgag
     1861 acttcgcaac gggaaggcgg gaagttggcg gctataataa gtgacctcgt ctcgcagtgg
     1921 gcacttagca tggggttatt gtcgttgctg cgcggctcga tcgtcgtcgc gctggccgtc
     1981 gtggcggcgc cgagcggcgc ggccacgttc gcggacctct acaccgtcaa gattgcaccg
     2041 gatcccgccg ccaccgatcg cgagaagacc gcgacgcagg ctgcgatggc gcggctgttg
     2101 atccgcgtga cgggcagccg caatgcgccg ctcgagcccg cattgcagcc gctgatcgcg
     2161 gcgccggaag atttcctgac gacgcgcggc tccgatcgcg aaggccgctc gatcgtcggt
     2221 tttcgccgcg gccaagtcga gagggtgttg acggagcgcg gcatgcgcgt gtggggcccc
     2281 gagcggccgc tgacgttgtt gtggatcgcc gtcgacgacg gcgccggcgg gcgcgcgttg
     2341 cttggcgcga acgaggcggc ccagttcggg gagccggcca cgccgcaaat gacgcagttg
     2401 ctggcaacga tacgcgagga agtggttgcc gcggcggacg agcgcggatt gccgatcgca
     2461 tggccgctgc tcgacctgca agacctgaac gccgtgacgt tcatcgacgt ctcggggcgg
     2521 ttcgacgacc gcatcgtcgc cgcctcggcg cgttaccgtg ccgacgcagt gctgatcggc
     2581 aacgtgcggc ccggcatcct cggcaacgag gtggactggt tgttcgtgaa cggcaccgag
     2641 cggcaagcgc tgccggtgac cgccgtgcgc gacgggctcg acgccgcggc cgatcgctac
     2701 gccgccgagc tcagcaccgt cggcggcgcg agcgtcgccc agttgaccgt gcgcgacgtg
     2761 cgaacgccgg ccgactacgg ccgcgtcatg agctacctcg agcgacagag cgtgctcgaa
     2821 agcgtcgacg tcgagtcgta cggggacggg actctaagct tgcgcgtcgc ggcgcgcggc
     2881 gacgcgcgcg tgctcgagcg cgtgctcgcg ctcggcggcg tgctgcgtcc ggccggcggc
     2941 ggcggtgcgc cggggtcgtt gacgttcgac gtcgtgggag ccggtagcgc gccatgagct
     3001 tgaggttcgt gccgaacgcc atttgcatcg cgcgcatgct gctcgtcgcg ccgctcgtgt
     3061 cgtggatcgt ccatggccgg ttcgccgccg cgctgctcgt gctcgtcgtc gcgggcttgt
     3121 cggacggcct cgacggctat ttggcgaagc gcttcgattg gcgcacgcgc ctcggcggcc
     3181 tgctcgatcc ggcggccgac aagctgctgc tcgtcagcac ttttgcggcg ttgagctacg
     3241 tcggcctgat tcccgtggag ctgacgatcg tcgtgatcgg ccgcgacgtc gtcatcgtgc
     3301 tgggcgccat ctgttatcag tggttcatcg cgcccgtgca aggcgaacct gcggcgatca
     3361 gcaagctgaa caccgcgtgc cagctcggca tgctgttctt cacgctgacg aacgcggcgt
     3421 tcgcatggcc gccgccggtg tcgctgctcg tgctcggcgc ggccgtcgtg ttcacgagca
     3481 tcgtcagcgg cctgacttac gtgctgcgct ggagcgcccg cgcctggcgc gttgcgcacg
     3541 gcgccacggc ctaggagctt cgttgggtca gctaccgctc gcgttggcgc tcgctgacta
     3601 cgcgagcttc gctaccttcg tcagcggcag caatgtggcc gccgtcgagc acgtgcgcag
     3661 tttggcggcg ggccgcggcg acacggtctg gctgttcggc gctgccggcg ccggcaagag
     3721 tcacttgctg caggccgcct gccgcgccg
//
LOCUS       DATLEE010000496         6702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_569568, whole genome shotgun sequence.
ACCESSION   DATLEE010000496 DATLEE010000000
VERSION     DATLEE010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569568"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VM692_10775"
     CDS             complement(<1..590)
                     /locus_tag="VM692_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUQ52694.1"
                     /translation="MSVDLLALARQRFGFKSFRPGQEPAIRALVDGHDVLAVLPTGAG
                     KSAIYQIAGGVLPGPTVVVSPLIALQQDQAQKLETPRSGGAAIVNSAVAQTAQHDTFV
                     DLAGDQLEFVFLAPEQLARADVLEKLNAARPSLLVIDEAHCISEWGHDFRPDYMRIGA
                     VIAALGRPRVLALTATAPPDVRKEIIARLALESPHVV"
     gene            921..1625
                     /locus_tag="VM692_10780"
     CDS             921..1625
                     /locus_tag="VM692_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52695.1"
                     /translation="MGDIGSAFAEAARLIAAADATLFGIVLLSLEVSLTAVAAATAIG
                     LPLGAALAIRRFPGRQILLVTLNALMGLPPVVVGLVVYILLSRAGPLGALGLLFTPPA
                     MVIAQAILVTPIIAGLSRQVLEDAWHEYEEQLRSLGATSSTAAATLVWDARYSLVTVV
                     LAGFGRAAAEVGAVMIVGGNIDGVTRVMTTAISLETSKGDLPLALGLGMILVAVVFLI
                     NAAAQVIKSAAVRRYG"
     gene            1576..2340
                     /locus_tag="VM692_10785"
     CDS             1576..2340
                     /locus_tag="VM692_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HUQ52696.1"
                     /translation="MRRPKSSSRRPSAATASAVLPLKLEDVSFVAGGRAIIDRVSCEL
                     AAGPRTVILGANGSGKSVLMRLCHGLLTPTSGRIAWRGSNDPRRPRAQAMVFQRPVML
                     RRSALGNVTYGLKLARVGKGERDARAREVLDAVGLSLIANRPARVLSGGEQQKLALAR
                     AWALRPEVLFLDEPTANLDPGATREVERIVDQIHASGTKIVMTTHNLGQARRLADEVL
                     FMSAGRVVDHAPVERFFAAPSTAEAAAFIKGELPWS"
     gene            2331..3164
                     /locus_tag="VM692_10790"
     CDS             2331..3164
                     /locus_tag="VM692_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUQ52697.1"
                     /translation="MELRSNVLRTAILLFAIACAPSALAQRFITVASTTSTEQSGLFK
                     HLLPIFERQTGIEVRVVALGTGQALDLARRGDADVVFVHAKAAEEEFLREGHGVRRYP
                     VMYNDFVIVGPRADPARIAGGADTLDALRKIKSAAAPFVSRGDRSGTHIAEVNLWTMA
                     GVDIAREKGPWYRETGQGMGPALNTASSMNAYLLSDRATWLAFMNRGELAILVEGDKR
                     LMNQYGVMLVNPEKHSNVKRADGQAFIDWVVSPEGQAAIADYKIAGEQLFFPNAAEPG
                     A"
     gene            complement(3174..3785)
                     /locus_tag="VM692_10795"
     CDS             complement(3174..3785)
                     /locus_tag="VM692_10795"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HUQ52698.1"
                     /translation="MSAGSGLVLGLVLAGGRSVRFGGEKAIALLEGKPLLAWAAERLR
                     TVCATVGVNVRPGTEAEAVAKAAGLPTLYDDAGDATGPLAGVKVGLIWAEKHGARALA
                     VSPCDAPLLPQDLYVRLLERAGEGAAMAETRDGWQPLCAIWPVGALAAVRAALAGGAH
                     PPTWQVLESIGAQKVLFDDSAAFANVNTRADLAAVESARSGRA"
     gene            complement(3782..4282)
                     /gene="mobB"
                     /locus_tag="VM692_10800"
     CDS             complement(3782..4282)
                     /gene="mobB"
                     /locus_tag="VM692_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HUQ52699.1"
                     /translation="MRLIGLAGWSGSGKTTLLVNLLPVLIARGLTVSTLKHAHHAFDV
                     DEPGKDSYSHRLAGAHEVLISSARRWALMHELRAAAEPTLAELVPHLSPVDLVIVEGF
                     KAETHAKLEVHRGVVGKPLLYPNDANIVALASDERPPSTHLPFAALDDIEAIAALVVE
                     LARPYP"
     gene            complement(4285..>6702)
                     /gene="fdhF"
                     /locus_tag="VM692_10805"
     CDS             complement(4285..>6702)
                     /gene="fdhF"
                     /locus_tag="VM692_10805"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HUQ52700.1"
                     /translation="DEHGHQHEEAALDLTNPAIAVNLDACISCNACMRACREVQVNDV
                     IGMGLRGSHAVPVFDIHDPMGLSTCVTCGECVQACPTGALYEKSLMDKTSTKRAVQKF
                     DKVVNTLCPFCGVGCQTSVAVKGNRIVQVDGRNGYANENRLCVKGRFGYDYVSSPERL
                     TKPLVRRDDAPKSDADVDKPFADRFSVFREATWEEALTRAAAGLKQILDERGGKALSG
                     FGSAKGSNEEAYLFQKLVRTGFETNNVDHCTRLCHASSVAALMEGIGSGAVSAPFNAA
                     MDADCVIVIGARPTVNHPVAATFFKQAAKRGTKLIVMDPRGQDLMRHATHALKFKPGS
                     DVALLNSLLHTIIEEKLYDETYIREHVSGFESLKAKIKDFSPEAMAEVVGVDANTLRD
                     VARIYATSKASIIFWGMGISQHTHGTDNARCLIALALITGQIGRPGTGLHPLRGQNNV
                     QGASDAGLIPMYFPDYRSVEKLDIRGWYENFWGQTLDPKRGLTVVEIMDAVHEGEITG
                     MYIMGENPAMSDPDQDHARAALAKLEHLVVQDIFLTETAWYADVVLPASAHAEKWGTF
                     TNTNRQVQMGRPALALPGDARQDWELIVELARRIGLDWKYDHVSDVYDEMAETMPSLK
                     NISWERVDREDSVIYPAKAPDVPGDKIAFTDGFPTADGRGKIVPADLLPPDELPDKEF
                     PLVLTTGRLLEHWHTGSMTRRAGVLDAIEPVGIAAMNPRDMQKRGLQQGDRITVETRR
                     GVLEAILRPDREVAEGMVFMPFCFAESPANRLTNPKLDPFGKIPEFKYCAARVERAEQ
                     AATAATG"
BASE COUNT         1047 a         2278 c         2242 g         1135 t
ORIGIN      
        1 acgacgtgcg gcgactcgag ggcgaggcgc gcgatgatct cttttcgcac gtccggcggc
       61 gccgtcgcag tgagtgccaa gacgcgcggc cggccgagcg ccgcaatcac ggcgccgatg
      121 cgcatgtagt cggggcggaa gtcgtggccc cattcgctga tgcagtgcgc ttcgtctatg
      181 acaagcagcg aaggccgcgc cgcgttcaac ttttcgagca catccgctcg cgcgagctgc
      241 tccggcgcca ggaaaacgaa ttcgagctgg tcgccggcga ggtcgacgaa ggtgtcgtgc
      301 tgcgccgtct gggcgacggc cgagttcacg atagcggcgc ctccggagcg cggggtttcg
      361 agcttttgtg cttgatcttg ctgcaacgca atcagcggcg acacgaccac ggtcggcccc
      421 ggcagcacac cgcccgcgat ctggtagatc gcggatttgc cggcgcccgt tggcagcacg
      481 gccagcacgt cgtgcccgtc gacgagcgcg cggatcgcgg gctcctggcc cgggcgaaat
      541 gacttgaagc cgaagcgttg gcgcgcgagc gcgagcaagt ccacgctcac tcgacacccc
      601 tccggcgccg caccgcagcg aatcgccctc gcaggaacag gaccttcgaa tgatcgtgaa
      661 tgcgcacgcg aagcgccgaa gtgcaagcgc cgtgccacgg ctcgcgacgc gcttttcagc
      721 cgttcgcgcg ttgcagtttc caagggcgat cccaaaggtt gaggagtagg aagcaatcgc
      781 tcgagtccca aacgacgctc gcaccaccgg cgacaagcgc tgaccgcgcg cgggtatctc
      841 ctgtatcgtg cccgcctggc accccgccgc ggctgcgcag ctagagtctc tatcctgtcc
      901 atgcaatcga agcacaccga atgggcgaca tagggtcggc attcgccgaa gccgcgcgtc
      961 tgatcgcggc cgcggacgcg acgctattcg gcatcgtgct attgagcctc gaagtgagcc
     1021 tcacggccgt cgccgcggcg acggcgatcg gcttgccgct cggcgccgcc ctcgcgattc
     1081 gccgctttcc gggccgccaa atattgctcg tgacattgaa cgcgctcatg ggtctgccgc
     1141 ccgtggtcgt cggcttggtc gtgtatatat tgctgtcgcg ggccggaccg ctgggcgctc
     1201 tggggctgtt gttcacgccg ccggcgatgg tcatcgcgca ggccatactc gtgacaccga
     1261 tcatcgcggg gctgtctcgc caagtgctcg aagacgcctg gcacgaatac gaagagcagc
     1321 tgcgatcgtt gggggcaacg tcctcgactg cggcggcgac gttggtgtgg gacgcacgct
     1381 actcgctcgt gaccgtcgtc ctcgcgggtt tcggccgcgc cgccgccgag gtcggcgccg
     1441 tcatgatcgt cggcggcaac atcgacggcg tgacacgcgt gatgacgacg gccatctcgc
     1501 tcgagacgag caagggggat ctgccgttgg cgctcgggct cggcatgatc ctcgtcgccg
     1561 tcgtgtttct aatcaatgcg gcggcccaag tcatcaagtc ggcggccgtc cgccgctacg
     1621 gctagcgccg tgctgccgct caagctcgag gacgtctcgt tcgtcgccgg cggccgcgcg
     1681 atcatcgatc gcgtatcgtg cgagctcgcc gcggggccgc gaacggtgat cctcggcgcg
     1741 aacggctccg gcaagagcgt cttgatgcgc ctctgtcacg gcttgctgac gccgacctcg
     1801 ggccgaatcg cctggcgcgg cagcaacgat ccgcgtcggc cgcgcgctca agcgatggtg
     1861 ttccagcgtc ccgtcatgct gcgccgctcg gcactcggca acgtcaccta tgggctcaag
     1921 ctggcccgcg tcggcaaagg cgagcgcgac gcgcgagcgc gcgaggtgct cgacgccgtc
     1981 ggcttaagcc tcatcgcgaa tcgcccggcc cgcgtgctat cgggcggcga gcagcaaaag
     2041 ctcgcgctgg cgcgcgcgtg ggcgcttcgt cccgaggtct tgtttctcga cgagccgacg
     2101 gcgaacctcg atcccggcgc cacgcgcgag gtcgagcgca tcgtcgacca gattcacgcg
     2161 agcggcacca agatcgtcat gacgacgcac aacctcggcc aagctcggcg gctcgccgac
     2221 gaggttttgt tcatgagtgc cgggcgagtc gtggatcacg cacccgtcga gcgtttcttc
     2281 gccgcgccgt cgacagcgga agcggcggcg ttcatcaaag gagagctgcc atggagttga
     2341 ggagcaacgt cctgcgcacc gcgatcctgc tcttcgccat cgcttgcgcg ccgagcgcgc
     2401 tggcgcagcg gttcatcacg gtcgcgtcga cgacgtccac ggagcagtcc gggctgttca
     2461 agcacctgct gccgatcttc gagcggcaaa cgggcatcga ggtgcgcgtt gttgcgctcg
     2521 gcaccgggca agcgctcgat ctcgctcggc gcggcgacgc cgacgtcgtc ttcgtgcatg
     2581 cgaaggccgc cgaggaagag tttctgcgag aaggccatgg cgtgcggcgc taccccgtca
     2641 tgtacaacga cttcgtcatc gttgggccgc gcgcggatcc cgcccggatc gcgggcggcg
     2701 ccgacacgct cgacgcgctg cgcaagatca agagcgcggc cgcgccgttc gtgtcgcgcg
     2761 gcgaccgcag cggcacgcac atcgccgagg tcaacctttg gaccatggcc ggcgtcgaca
     2821 tcgcccgcga gaagggcccg tggtatcgcg agaccggcca aggcatgggg ccggcgctga
     2881 acacggcgtc ctcgatgaac gcttatttgt tgtccgatcg cgcgacgtgg ctcgcgttca
     2941 tgaatcgcgg ggagctcgcg attctcgtcg aaggcgacaa gcgcctgatg aatcagtacg
     3001 gcgtgatgct cgtcaatccc gagaagcact cgaacgtgaa gcgcgccgac ggtcaggcgt
     3061 tcatcgattg ggtcgtctcg ccggaggggc aagccgccat tgccgactac aagatcgcag
     3121 gcgagcagct gttctttccg aacgccgccg agccgggcgc gtagcgtcgc gccctaggcg
     3181 cgccccgagc gcgccgactc gacggcggcg aggtctgcgc gcgtattgac gttcgcgaac
     3241 gccgcagagt cgtcgaacaa aaccttctgc gcgccgatgc tctcgagcac ttgccacgtc
     3301 ggcggatgag cgccgccggc caacgccgcg cgaaccgcgg ccagcgcgcc gacgggccag
     3361 atcgcacaca gtggctgcca cccgtcgcga gtctcggcca tcgcggcacc ctcgccggca
     3421 cgctcgagca accgcacgta gagatcctgc ggcagcagcg gcgcgtcgca tgggctcacg
     3481 gccaacgcgc gcgcgccgtg cttttcggcc cagatgaggc cgaccttgac accggcgagc
     3541 ggccccgtcg cgtcgccggc gtcgtcgtag agcgtcggca gcccggcggc tttcgcgacg
     3601 gcttcggcct cggtccccgg tcgcacgttc acgccgacgg tcgcgcagac cgtgcgcaac
     3661 cgttccgccg cccacgcgag cagcggcttt ccctcgagca gcgcgattgc tttctcgccg
     3721 ccgaagcgca cggagcgccc gcccgcaagc acgagaccca gcaccaagcc ggaaccggcg
     3781 ctcatggata cggccgcgcg agctcgacta cgagagcggc gatcgcttcg atgtcgtcga
     3841 gcgccgcgaa cggcaggtgt gtactcggcg ggcgttcgtc actcgcgagc gcgacgatgt
     3901 tcgcgtcgtt cgggtacagc agcggcttgc cgaccacgcc gcggtgcacc tcgagcttcg
     3961 cgtgcgtctc ggctttgaaa ccttcgacga tgacgaggtc gacgggcgag aggtgcggca
     4021 cgagctcggc cagcgtcggc tccgccgcag cgcgcagctc gtgcatcagt gcccaacgac
     4081 gcgcgctcga aatcagcacc tcgtgcgcgc ccgcgagccg atggctgtac gaatccttgc
     4141 cgggctcgtc gacgtcgaac gcgtgatgcg cgtgcttcaa cgtcgaaacc gtgaggccgc
     4201 gcgcgatcag cacgggaagg aggttcacga gcagcgtcgt tttgccgctg ccgctccaac
     4261 ccgcgagccc gatcaggcgc acgatcagcc cgtggccgcc gtcgccgcct gctcggcgcg
     4321 ctccacgcgt gccgcgcagt acttgaactc ggggatcttg ccgaacgggt cgagcttcgg
     4381 attcgtcaag cgattcgccg ggctctccgc gaagcagaac ggcatgaaca ccatgccctc
     4441 ggcgacctca cgatcggggc gcaagatcgc ctcgagcacg ccgcgccgcg tctcgacggt
     4501 gatgcggtcg ccttgctgca gcccgcgctt ctgcatgtcg cgcggattca tcgcggcgat
     4561 gccgaccggc tcgatcgcgt cgagcacgcc tgcgcggcgc gtcatcgagc ccgtgtgcca
     4621 gtgctcgagc agccggcccg tcgtcagcac gagcgggaat tccttgtccg gcaactcgtc
     4681 gggaggcagg aggtcggcgg gcacgatctt gccgcggccg tccgccgtcg ggaaaccgtc
     4741 cgtgaacgcg atcttgtcgc cgggcacgtc gggcgccttg gcgggataga tgaccgagtc
     4801 ttcgcggtcg acgcgttccc aggagatgtt cttcagcgac ggcatcgtct cggccatctc
     4861 gtcgtagacg tcggaaacgt ggtcgtactt ccaatcgagg ccgatgcgcc gcgcaagctc
     4921 gacgatgagc tcccagtctt ggcgcgcgtc gccgggcaac gcgagcgccg gccgacccat
     4981 ctgcacctgg cggttcgtgt tcgtgaacgt gccccatttt tcggcgtgcg ccgaagccgg
     5041 cagcacgacg tcggcatacc acgccgtctc cgtcaggaag atgtcttgca cgacgagatg
     5101 ctcgagcttc gcgagcgccg cccgcgcgtg gtcctgatcg gggtccgaca tcgcggggtt
     5161 ctcgcccatg atgtacatgc ccgtgatctc gccttcgtgc acggcatcca tgatctcgac
     5221 gacggtcagg ccgcgcttcg ggtcgagcgt ctgaccccag aagttctcgt accaaccgcg
     5281 gatatcgagc ttctcgacgg accggtagtc cgggaagtac atcgggatca gccccgcgtc
     5341 cgatgcgccc tgcacgttgt tttggccgcg cagcggatgc aggcccgtgc cggggcgtcc
     5401 gatctggccc gtgatcaacg cgagcgcgat caagcagcgc gcattgtcgg tgccgtgcgt
     5461 gtgctgcgag atgcccatgc cccagaaaat gatcgacgcc ttcgacgtcg cgtaaattcg
     5521 cgcgacgtcg cgcaaggtgt tggcgtcgac gcctacgacc tcggccattg cttccggcga
     5581 aaagtctttg atcttggcct tcagcgactc gaagcccgag acgtgctcgc gaatgtacgt
     5641 ctcgtcgtac agcttctctt cgatgatcgt atgcagcagc gagttcagca gcgcgacgtc
     5701 gctgccgggc ttgaacttca gcgcgtgcgt cgcgtgccgc atgaggtcct ggccgcgcgg
     5761 gtccatgacg atgagcttcg tgccgcgctt cgccgcttgt ttgaagaacg tcgccgcgac
     5821 cgggtgattc acggtcggtc gcgcgccgat cacgatgacg cagtcggcgt ccatggccgc
     5881 gttgaacggc gccgagacgg cgcccgagcc gatgccttcc atgagcgcgg cgaccgacga
     5941 cgcatggcag aggcgcgtgc agtgatcgac gttgttggtc tcgaagcccg tgcgcacgag
     6001 cttctggaat agataagcct cctcgttgga gcctttcgcg ctgccgaagc ccgataacgc
     6061 cttgccgccg cgctcgtcga ggatttgctt gaggccggcg gcggcgcgcg ttaacgcctc
     6121 ttcccacgtc gcttcgcgga acacgctgaa gcggtcggcg aacggcttgt cgacgtcggc
     6181 gtcgctcttc ggcgcgtcgt cgcggcgcac gagcggcttc gtcagacgct cgggcgacga
     6241 aacgtagtcg tagccgaagc ggcctttgac gcaaagccgg ttctcgttcg cgtagccgtt
     6301 gcggccgtcg acctgcacga tgcgattgcc cttcacggcg accgaggttt ggcagccgac
     6361 gccgcagaac ggacacagcg tgttgaccac cttgtcgaac ttctgcacgg cgcgcttcgt
     6421 ggacgtcttg tccatcagcg acttctcgta caacgcgccc gtcgggcacg cctgcacgca
     6481 ttcgccgcac gtcacgcacg tcgaaagacc catcgggtcg tggatgtcga acaccggcac
     6541 ggcgtggctg ccgcgcagcc ccatgccgat gacgtcgttg acctgcacct cgcggcacgc
     6601 gcgcatgcac gcgttgcacg agatgcacgc gtcgagattc acggcgatgg caggattcgt
     6661 gaggtcgagc gccgcttctt cgtgctgatg cccgtgctcg tc
//
LOCUS       DATLEE010000497         2074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5036185, whole genome shotgun sequence.
ACCESSION   DATLEE010000497 DATLEE010000000
VERSION     DATLEE010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5036185"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VM692_10810"
     CDS             <1..473
                     /locus_tag="VM692_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52701.1"
                     /translation="QVAVVLHADGERVRVDDDATGATATLRATPLDLLRLVNAEGVSG
                     VKRTHAELKGDLQVAERYAQLLKLARPDLEEEASKWLGDVPAHALGEVARGVGSWLER
                     AGAALRMNTAEYLQEESRAMPAPLEAHAFYADVERLRDDVERAAARLALLERKR"
     gene            479..>2074
                     /gene="ubiB"
                     /locus_tag="VM692_10815"
     CDS             479..>2074
                     /gene="ubiB"
                     /locus_tag="VM692_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HUQ52702.1"
                     /translation="MRLRQLGRMLAIQRVLVRHGLDEIVWRTHLFRPLAWLGKLLSLG
                     RRRAPLGVRLRTALEELGPIFVKFGQALSVRPDLLPPDVAAELAKLQDQVPPFAGDDA
                     AAALALAFGRPAEEVFAEFDRTPLAAASIAQVHAARLPTGEAVVVKILRPNVATLILR
                     DVEVLYAIAGLAERYWPEAHRLHPVEIVAEFERTLAVELDLMREAANAAQLKRNFAGS
                     DLLYVPEIYWDFCRSNVLTIERIAGIQVDDIDALRAAGTDIRRLAENGVEIFFTQVFR
                     HNFFHADMHPGNIFVDVTDPARPQYVAVDFGIVGTLDERDQHYLAENFLAFFRRDYRR
                     VAALHVDSAWVPAGTRVDELEAAVRAVCEPIFNKPLKDISFGLVLMRLFQTARQFNME
                     IQPQLVLLQKTLLAIEGLGRQLYPELDLWQTAKPVLEDWMRERHDPRTRLKQLVAAWP
                     EISEDLAMLPRVLHRALRRIDAEDAAVRRQQRAPVIIAERRRPTARIEQLVFGGALLI
                     AGVLWTGLASPPWVGWAGAAVGLA"
BASE COUNT          294 a          738 c          716 g          326 t
ORIGIN      
        1 tccaagtagc cgtcgtgctg catgcggacg gcgagcgcgt gcgcgtcgac gacgacgcga
       61 ccggcgcgac ggcgacgttg cgcgccacgc cgctcgatct gctgcggctc gtgaacgccg
      121 aaggcgtcag cggcgtgaag cgcacgcacg ccgagctcaa gggcgatctg caagtcgccg
      181 agcggtacgc gcagctgctg aagctcgcgc gtcccgacct cgaagaggaa gcgtccaagt
      241 ggctcggcga cgtgccggcg catgcgctcg gcgaggtcgc gcgcggcgtc ggctcatggc
      301 tcgagcgcgc cggcgccgcg ctgcgcatga acacggccga gtacttgcag gaggaaagcc
      361 gcgcgatgcc cgcgccgctc gaggcgcacg cgttctacgc cgacgtcgag cgcctgcgcg
      421 acgacgtcga gcgcgccgcg gcacgtctcg cgctgctcga gcgcaaacga tgacgcgtat
      481 gcggctgcgg cagctcgggc gcatgctggc gatccagcgc gtgctcgtgc ggcacggcct
      541 cgacgagatc gtttggcgca cgcatttgtt tcggccgctg gcgtggctcg gcaagctgct
      601 ctcgctgggc cgcaggcgag cgccgctcgg cgtgcgcctc cgcacggcgc tcgaggagct
      661 gggcccgatc ttcgtgaagt tcggccaagc cctttccgtg cgcccggact tattgccgcc
      721 cgacgtcgcg gccgagctcg cgaagctgca ggatcaagtg ccgccgtttg ccggcgacga
      781 cgcggccgcg gcgctcgcgc tcgcgttcgg ccggcccgcc gaggaagtgt tcgccgagtt
      841 cgaccgcacg ccgttggccg cggcctcgat cgcgcaggtg cacgccgctc gcttgcccac
      901 aggcgaggcc gtcgtcgtca aaatcctgcg gccgaacgtc gcgacgctga tcctgcgcga
      961 cgtcgaggtg ctatacgcga tcgccggctt ggcggagcgg tattggcccg aagcgcaccg
     1021 tctgcacccc gtcgagatcg tcgccgagtt cgagcgcacc ttggccgtcg agctcgacct
     1081 catgcgcgaa gcggcgaacg ccgcgcagct gaagcgcaac ttcgcgggct cggacctgtt
     1141 gtacgtgccc gagatctact gggacttctg ccggtcgaac gtgctgacga tcgagcgcat
     1201 cgccggcatc caggtcgacg acatcgacgc gttgcgcgcc gcgggcacgg acatccggcg
     1261 gctcgccgag aacggcgtcg aaattttttt cacgcaggtg ttccgccaca atttctttca
     1321 cgccgacatg caccccggca acatcttcgt cgacgtgacg gaccccgccc ggccgcagta
     1381 cgtggccgtc gacttcggca tcgtcggcac actcgacgag cgcgaccagc actacctcgc
     1441 cgaaaatttc ctcgcgttct tccgccgcga ctaccgccgc gtcgccgcgc tgcacgtcga
     1501 ctcggcctgg gtcccggccg gcactcgcgt cgacgagctc gaggctgccg tgcgggccgt
     1561 ctgcgagccg atcttcaaca agccgctgaa ggacatctcg ttcggcctcg tgctgatgcg
     1621 cctgttccag accgcgcggc agttcaacat ggagatccag ccgcagctcg tgctgctgca
     1681 aaagacgctg ctcgcgatcg agggcttggg ccgccagctc taccccgagc tcgatctttg
     1741 gcagaccgcg aagcccgtgc tcgaggactg gatgcgcgag cgtcacgacc cgcgcacgcg
     1801 cttgaagcag ctcgtcgctg cgtggcccga gatcagcgag gatctcgcga tgttgccgcg
     1861 cgtgctgcac cgcgcgctgc ggcgcatcga cgccgaggat gcggccgtgc gccgccagca
     1921 gcgcgcaccg gtcatcatcg ccgagcgtcg ccgaccgacg gcgcggatcg agcagctcgt
     1981 cttcggcggc gcgctgctca tcgccggagt cctttggacg gggctcgctt cgccgccctg
     2041 ggtcggctgg gcgggcgcgg cggtcggctt ggcc
//
LOCUS       DATLEE010000498         2375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_570894, whole genome shotgun sequence.
ACCESSION   DATLEE010000498 DATLEE010000000
VERSION     DATLEE010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570894"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1737
                     /locus_tag="VM692_10820"
     CDS             157..1737
                     /locus_tag="VM692_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ52703.1"
                     /translation="MAIVGAGEATAQTAAELMAEGNALVRSGVYRTALLRYREAAAAG
                     FDTPLLHFNVGVVHYKLGNFADAAAEFAAAAGEPALTALASYNRGLALRAAGDGAAAT
                     AAFTAAAESADARDLRRLAANAAQSRPAAEPEQARVTRRFEPDAAAADKRIGELFLTA
                     AARLGQDDNVYRTPSSAYVDLANPLAPTVAPVVHSASFMPAELHAAYLLGNEAGDTEF
                     MFRYDLDGAFYDAEFSNANEIDQRFSMGADIVLGEREKRRRTVDTEFFVSTHDETNYD
                     PDNGLERDIDLISGVVEDISDRFSYKGSGVRGEFEQTLGRITWGFELRFERDEYGRTE
                     AVANFDHDYFYTGVDVDYAFSDAMTLRLGLRGYRTLFDTRPARDLNGELLDTNPAQEY
                     GHLGVQLGVTRQLGAAVELAADYLRLQRTDEFLGYYDYTQDVLRLGAAFRPTPRFDIE
                     LAAVARSYDYSHAFAFHVAAGGPRELEEVGLTLDAEFRVTRRLTLKAALDSLDVTSTD
                     ARAEYLRTQTMLGVEWRK"
     gene            complement(1760..>2375)
                     /locus_tag="VM692_10825"
     CDS             complement(1760..>2375)
                     /locus_tag="VM692_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52704.1"
                     /translation="GTVDTPRVTAAEPRNVFDAAALAAVARRRYPAAEGRDPQVVKER
                     IEFAPPRVAREAAVAAAAGPRNQCVRPDAEYNYGEMVDVGLVNACNEALMVFGCAQGA
                     GRDLGRWVCSTSEQRGDILVPAGDQRLGRRYAAGNAADVRTYTYRDSYSLTRAPNSQI
                     WWVACAEADAACRSDARLWTRAVGGQDATVDPQARSTIAVASSN"
BASE COUNT          352 a          824 c          812 g          387 t
ORIGIN      
        1 ttttgtcgtc gacctgtccg tcgcgccgcc ttaaggactg cctccgggcc ctgtgacggg
       61 cgtcctgttc gggccccgcg gccgcgccta gagtggcgca aacccggccg cgaggcttcc
      121 gtgctgcttc acaagcgatt gatatctctc gccattttgg ccattgtcgg cgccggcgag
      181 gccaccgccc agacggccgc cgagctcatg gcggagggca acgctttggt gcgctccggc
      241 gtctaccgaa cggccctgtt gcgctaccgc gaggcggcgg ccgccggttt cgacacgccg
      301 ctgctgcact tcaacgtcgg cgtcgtgcac tacaagctcg gcaacttcgc cgacgcagcg
      361 gcggagttcg cggctgccgc cggtgagccc gcgctgacgg cgctggccag ctacaaccgc
      421 ggcttggcgc tgcgcgcggc cggcgacggt gccgctgcca cggccgcgtt cactgccgcg
      481 gccgagagcg ccgacgctcg cgatctgcgc cggctcgccg cgaacgccgc gcagtcgcgg
      541 ccggctgccg agcccgagca agcgcgcgtg acgcggcggt tcgaacccga cgccgctgcg
      601 gcggacaagc gcatcggcga gctgttcctg acggcagcgg cgcgcctcgg tcaagacgac
      661 aacgtctatc gcacgccatc gagcgcttac gtcgacctcg ccaatccgtt ggcgccgacg
      721 gtcgcgccgg tcgtgcactc ggcgagcttc atgccggccg agctgcatgc ggcctacctg
      781 ctcggcaacg aggcgggcga tacggaattc atgttccgct acgatctcga tggcgcgttc
      841 tacgacgccg agttcagcaa tgccaacgag atcgatcagc gcttctcgat gggtgccgac
      901 atcgtgctcg gcgagcggga gaagcggcgc cgcacggtcg ataccgaatt tttcgtcagc
      961 actcatgacg agacgaacta cgaccccgac aacggcttgg agcgcgacat cgaccttatc
     1021 agtggcgtcg tagaggacat cagcgaccga ttctcgtaca agggttccgg cgtgcgcggc
     1081 gagttcgagc aaacgctcgg ccggatcacg tggggcttcg agctccgatt cgagcgcgac
     1141 gagtacgggc gcacggaggc cgttgcgaac ttcgaccacg attactttta tacgggtgtc
     1201 gacgtcgatt acgcgttcag cgacgccatg accctgcggc tcgggctgcg cggctaccgc
     1261 acactgttcg acacgcggcc cgcgcgcgac ttgaacggcg agctgctcga tacgaacccg
     1321 gcgcaggagt acggccatct cggcgtgcag ctcggcgtca cacgtcagct gggcgccgcc
     1381 gtggaactag cggccgatta tttgcgcttg cagcgcaccg acgagttcct cggttactac
     1441 gactacacgc aggatgtgct acgcctcggt gccgcgttcc ggccgacgcc gcgtttcgac
     1501 atcgagcttg ccgctgttgc gcgcagctac gactacagcc acgcgttcgc atttcacgtc
     1561 gcggctggcg gcccgcgcga gctcgaggag gtcggattga cgctcgacgc ggagtttcga
     1621 gtcacgcggc ggctcacgct gaaggccgcg ctcgactcgc tcgacgtgac gtcgacggac
     1681 gcccgcgccg aatacctgcg cactcagacg atgctgggcg tcgagtggcg caagtagtct
     1741 tcggcagccg ccgaagcggc tagttggagc tcgcgacggc gatcgtgctg cgcgcctgcg
     1801 gatcgacggt ggcgtcttgg ccgccgacgg cgcgcgtcca aaggcgcgcg tcggaccggc
     1861 aagccgcgtc cgcttcggcg caggccaccc accaaatttg cgagttcggc gcgcgcgtca
     1921 gcgagtagct gtcgcggtag gtgtacgtgc ggacatcggc ggcattgccg gccgcgtagc
     1981 ggcgcccgag ccgttggtcg ccggccggca cgaggatgtc gccgcgctgc tcggacgtgc
     2041 tgcataccca gcgccccaag tctcggccgg cgccttgagc gcaaccgaac accatcagtg
     2101 cctcgttgca cgcgttgacg aggccgacgt cgaccatttc gccgtagttg tactcggcat
     2161 ccggccgcac gcattgattg cgcggccccg ccgcagcggc gacggcggcc tcgcgcgcga
     2221 cgcgcggcgg cgcgaattcg atgcgctcct tgacgacctg cgggtcgcgt ccttcggccg
     2281 ccggatatcg gcggcgagcg acggccgcga gcgccgcggc gtcgaagacg ttgcgtggct
     2341 ccgccgccgt gacgcgcggc gtatcgacgg tgccc
//
LOCUS       DATLEE010000499         3386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4272618, whole genome shotgun sequence.
ACCESSION   DATLEE010000499 DATLEE010000000
VERSION     DATLEE010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4272618"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VM692_10830"
     CDS             <1..526
                     /locus_tag="VM692_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUQ52705.1"
                     /translation="FVRTRGASQAVGSGTINEAIESLGVPLRRVVVTPMAQVARDPFW
                     RQQLFSTWFTAFGAAALTLAAIGLYGVLAFVVGQRRREIGIRMAVGARRRQVLALVLR
                     QGAALAGIGIAVGLVAAYALARSLQSLLFGVDAFEWSVFFAVAATLGAVAIAASFAPA
                     LRAARVDPNTLFKS"
     gene            complement(537..1943)
                     /locus_tag="VM692_10835"
     CDS             complement(537..1943)
                     /locus_tag="VM692_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUQ52706.1"
                     /translation="MPRIPVRAAVALTLVLPPLLAARAQPHAQAEAQALALAQETIAL
                     RSVQGPGNRTIDVARVYARALREAGWPERDLEIVPVDDTAFFIATWPGSDASLAPVVL
                     SAHMDVVEARREDWERDPFTPVVENGFLYGRGAADTKFDASLAIASAVELRRQGFRPK
                     RSIVIAYSGDEETTMKTSKLIADRLADAHVVLNIDSPSGVLSEQTGEPLYWQWQGAEK
                     TYIDFELEVTNPGGHSAQPRADNAIAQMSQALERISAYHFTPEQNDITRSYFAKAAAI
                     QPNASVAAAMRAFAANVNDAQALEVLRADSVMIGRTGTTCVPTMVSGGHAVNALPQRV
                     TAIVNCRVFPGHSSAEIKAELERVAATPEVRITDVTGDTAIASDASPLRAEFVEAFEK
                     SITAAWGKVPVYPTQASDASDSMWYRAAGVPSYGASATFIKESDDFMHGLNERIPLLN
                     VRPGVTYYLSVLTELSSR"
     gene            complement(1943..2434)
                     /locus_tag="VM692_10840"
     CDS             complement(1943..2434)
                     /locus_tag="VM692_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HUQ52707.1"
                     /translation="MAADFDLTRYQDPLTIQRVLLNTKTVAIVGLSRNTLRASHFVGF
                     YLRRHGYRVIAVNPREKEIFGEPSYATLKDVPERIDMVNVFRSPDALPEIARETVAVG
                     AKSLWCQFNVVNEEGARIAEDSGVTVIMDRCIKVEHARYVGRMHWLGFNTQRITSVRG
                     GLQ"
     gene            complement(2437..>3386)
                     /locus_tag="VM692_10845"
     CDS             complement(2437..>3386)
                     /locus_tag="VM692_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HUQ52708.1"
                     /translation="LLRKMNVELTWVDPYDPDAWRSAVRENTKAFFAETIGNPGGNVL
                     DIEALAGIAHEHGVPLLVDNTFATPYLCRPIEWGADIVLHSATKFLGGHGTSLGGVVV
                     DSGTFNWSNGRFPVVAEPSPAYHGLAFHETFGTYGYLMKLRAETLRDLGAALSPFNAF
                     LFLQGIETLSLRMDRHVANALAIARWLEKHEQASNITYSGLSSSRYRPLVAKYLPRGT
                     GSVFSFDIEGGRDAGQEFIRAVSLWSHLANVGDAKSLVIHPASTTHRQLSEEELEAAG
                     VRAGTIRLAVGIENVDDLIWDLEQGFARARGAQPAKKGA"
BASE COUNT          516 a         1113 c         1161 g          596 t
ORIGIN      
        1 cttcgtgcga acgagaggcg cgtcgcaagc ggtcggctca gggacaatca acgaagcaat
       61 cgagtcgctg ggcgttccgc tgcggcgcgt cgtcgtcacg ccgatggcgc aagtcgcgcg
      121 cgatccgttc tggcggcagc aattgttcag cacctggttc acggcgttcg gcgcggcggc
      181 gctgactctc gcggcgatcg gcctgtacgg cgtgctcgcg ttcgtcgtcg gtcagcggcg
      241 cagggagatc ggcattcgca tggccgtcgg cgctcgccgc cgacaagtgt tggcgctcgt
      301 tcttcggcag ggcgcggcgc tcgccggcat cggcatcgcc gtcggtctcg tcgcggcgta
      361 cgccctcgcg cgctcgctgc agagcttgct gttcggcgtc gacgcgttcg agtggagcgt
      421 gttcttcgcg gtggccgcga cgctcggtgc cgtcgcaatc gcggccagct tcgcgccggc
      481 gctgcgagcg gcgcgcgtcg atccgaacac gctattcaag agctgagcgg ccgccgctat
      541 ctcgaagaca gctcggtcag cacgctgaga tagtaagtga ctcccggccg cacgttcagc
      601 agcgggatgc gctcgttgag gccgtgcatg aagtcgtcgg attccttgat gaacgttgcg
      661 ctcgcaccgt agctcgggac gccggcggcg cgataccaca tcgaatccga tgcgtccgaa
      721 gcttgagtcg gataaacggg aaccttgccc caggccgcgg tgatgctttt ctcgaacgcc
      781 tcgacgaact ccgcccgcag cggcgaggca tcggaggcga tggcggtgtc gcccgtcacg
      841 tcggtgatgc ggacctcggg cgtcgcggcg acgcgctcga gctcggcctt gatttccgcg
      901 ctcgaatggc cggggaatac acggcaattg acgatcgccg tgacgcgctg cggcagcgca
      961 ttcacagcgt gaccgcccga gaccatcgtc ggcacgcagg ttgtccccgt gcggccgatc
     1021 atgacggagt cggcgcgcaa tacttcgagg gcttgcgcgt cgttgacgtt cgcggcgaac
     1081 gcccgcatgg cggcggcaac tgacgcgttc ggctgaatcg ccgcggcctt cgcgaaatag
     1141 ctgcgcgtga tgtcgttctg ctcgggcgtg aagtggtacg cgctgatgcg ttcgagcgcc
     1201 tgcgacatct gggcgatcgc gttgtcggcg cgcggctgcg cactgtgccc gccggggttc
     1261 gtcacctcga gctcgaagtc gatgtaagtc ttctcggcgc cctgccactg ccagtacagc
     1321 ggttcgccgg tctgctccga cagcacgccc gatgggctgt cgatgttcag cacgacgtgc
     1381 gcgtcggcaa ggcggtcggc gatgagcttc gaggtcttca tcgtcgtctc ttcatcgccc
     1441 gagtaggcga tgacgatgct gcgcttcggc cgaaagccct gccgcctgag ctccacggcc
     1501 gaggcgatcg ccaacgacgc atcgaatttc gtgtcggcgg ctcctcggcc gtacaagaag
     1561 ccgttctcga cgaccggcgt gaacggatcg cgctcccagt cttcgcgtcg cgcctcgacg
     1621 acgtccatgt gagccgaaag cacgaccggc gctagtgacg cgtcgctgcc aggccacgtc
     1681 gcgatgaaga aggcggtgtc gtcgacgggg acgatctcga ggtcgcgctc gggccagccg
     1741 gcttcgcgca gcgccctcgc gtagacgcgc gcaacgtcga tcgtgcgatt gcccgggccc
     1801 tgaaccgagc gcagcgcgat cgtctcttgc gccaacgcga gcgcttgtgc ctcggcttgc
     1861 gcgtgcggct gcgctcgcgc cgccaacagc ggcgggagaa cgagcgttag ggcgacggcg
     1921 gcgcgcacgg gaatgcgcgg cactactgca acccgccccg cacggacgtg atgcgctgcg
     1981 tgttgaaccc gagccagtgc atgcggccga cgtagcgcgc gtgctcgacc ttgatgcagc
     2041 ggtccatgat gaccgtcacg ccgctgtcct cggcgatgcg cgcgccttct tcgttgacga
     2101 cgttgaactg gcaccagagg ctcttcgcgc cgacggcgac ggtttcgcgc gcgatctccg
     2161 gcagcgcgtc cggcgagcgg aacacgttga ccatgtcgat gcgctccggc acgtccttca
     2221 aggtcgcgta gctcggctcg ccgaagatct ctttctcgcg cgggttaacg gcgatcacgc
     2281 gatagccgtg gcggcgaaga tagaacccga cgaaatggct cgcgcgcagt gtgttcctcg
     2341 acaggccgac gatcgcgacg gttttcgtgt tcagcagcac gcgctggatc gtcagaggat
     2401 cttggtagcg cgtgaggtcg aagtccgcgg ccatcgtcat gctcctttct tggcgggctg
     2461 ggcgccgcgc gctcgcgcga agccttgctc gagatcccag atcaggtcgt cgacgttctc
     2521 gatgcccacg gcgagccgga tggtccccgc gcgcacgccg gcggcctcga gctcttcctc
     2581 cgagagctgc cggtgcgtcg tgctcgcggg atggatcacg aggctcttcg catcgccgac
     2641 gttcgcgaga tgcgaccaca gcgagacggc gcgaatgaac tcctggcccg cgtcgcggcc
     2701 gccctcgatg tcgaacgaga acaccgagcc cgtgccgcgc ggcaagtatt ttgcgacgag
     2761 cggccgatag cggctgctgc tcaggcccga ataggtgatg ttcgacgcct gctcgtgctt
     2821 ctcgagccag cgcgcaatcg cgagcgcatt cgcgacgtgc cggtccatgc gcaacgacag
     2881 cgtctcgata ccttgcagga acaagaacgc gttgaacggc gacagcgccg cgcccaagtc
     2941 gcgcagcgtc tccgcgcgca gcttcatgag gtagccgtac gtgccgaacg tctcgtggaa
     3001 cgcgaggccg tgataggccg gcgacggctc cgcgacgacg gggaagcggc cgttcgacca
     3061 gttgaacgtg cccgagtcga cgacgactcc gccgaggctc gtgccgtggc cgccgaggaa
     3121 cttcgtcgcc gagtgcagca cgatgtcggc gccccattcg atcggccggc atagataagg
     3181 agtcgcgaac gtgttgtcga cgagcagcgg cacgccgtgc tcgtgcgcta tgcctgccag
     3241 cgcttcgatg tcgagcacat tgccgccggg gttgccgatg gtctcggcga agaacgcctt
     3301 cgtgttctcc cggaccgcgg agcgccacgc gtccgggtcg taagggtcga cccaggtcag
     3361 ttcgacgttc atcttgcgca gcaggt
//
LOCUS       DATLEE010000500         2808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2806080, whole genome shotgun sequence.
ACCESSION   DATLEE010000500 DATLEE010000000
VERSION     DATLEE010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806080"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VM692_10850"
     CDS             <1..246
                     /locus_tag="VM692_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUQ52709.1"
                     /translation="VVAKGKNLTEQFYCVSCHGATLAGQQHVPRLAGQQRQYLATQLR
                     GFKAGTRFDMDGNMTAAMQAVPDAEIEVLAEYLSSLK"
     gene            374..2389
                     /locus_tag="VM692_10855"
     CDS             374..2389
                     /locus_tag="VM692_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ52710.1"
                     /translation="MTVTATRIERDPLDLPVSIDVVGGRTIREDRPQVNLSEALNAVP
                     GIVVQNRYNYAQDLQISSRGFGARASFGVRGIRLIADGIPATMPDGQGQAATFNLDTA
                     ERIEVLRGPFSSMYGNSSGGVIQIFTADAPRDPTVTTSYYRGTFDTTKTNTQYGAQHG
                     PLNANVAYSLFETGGFRDHSAARREHGHAKLKFDGGSAGTLTVLADALHQPDTQDPLG
                     LTRAQVQQDRRQAGTNALAFNTRKSVAQSQVGLVHDLKLTSHDTLQTRFYVGDRQVTQ
                     YLGIPIATQAAPTHSGGVVDLDRGYWGGGLRWSRSNTLLGGPFDVHVGADYERMAEGR
                     RGYINSFGNQGNLKRDEHDTVYTADFYLQSEWRFHERWLLMAGVRRSNVHFNSRDFFV
                     TAANGDDSGGIDFSKTLPVAGVTFDVAPRVKLYANLGKGFETPTFAELAYRPDGTTGL
                     NFALRPSVSTNREIGSKFELTPTSRATVALFRIDVADEIVVASNTGGRTTFKNAAHTR
                     REGVELGLQGRWLDQFEASVAYTRIRARFTDAFTTSTGTPATIVTVNPGSRLPGVPQS
                     TLYGELVWRHARSGFHAAAEVRHNGSVYVDDQNSDAASSYTIGNVRFGFEQQGRGWRL
                     SEFVRVDNVTNREYVGSVIVAEANGRFFEPAPGRTWLVGVSASLTFR"
     gene            2403..2606
                     /gene="thiS"
                     /locus_tag="VM692_10860"
     CDS             2403..2606
                     /gene="thiS"
                     /locus_tag="VM692_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HUQ52711.1"
                     /translation="MLTVTINGESRQLPAAMPVQALIEELGLTGKRIAVERNGEIVPR
                     SEFAARSILDGDKLEIVVAVGGG"
     gene            2606..>2808
                     /locus_tag="VM692_10865"
     CDS             2606..>2808
                     /locus_tag="VM692_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HUQ52712.1"
                     /translation="MGILMKAFSAAALTVMFAAAAAAQEFPSRPVRFVVPLTAASGAD
                     IAGRIVGRQLQEMWKQTVLVDNRP"
BASE COUNT          498 a          960 c          896 g          454 t
ORIGIN      
        1 gtcgtcgcga aaggcaaaaa cctgaccgag cagttctatt gcgtctcatg tcatggcgcg
       61 acgctcgccg gccagcagca cgtgccgcgc ctcgccggac agcaacggca atatctcgca
      121 acgcagctgc gcgggttcaa ggcgggaacg cggttcgaca tggacggcaa catgaccgct
      181 gcgatgcagg ctgtgcccga tgctgaaatc gaagtcctcg ccgagtacct ttcgagcctc
      241 aagtagcagc gtgccgctga ggcctttccg tttttgcggg tgtgtgtcag ccgcgctcgc
      301 ctgcgccggc agtgcgtccg tcttcgcggc cgacgctgcg ccagcgacag cggagccggc
      361 cgttctgccg agcgtgaccg ttacggccac gcgcatcgag cgcgatcctc tcgatctccc
      421 ggtatcgatc gacgtcgtcg gcggacgcac gatccgcgaa gaccgcccgc aggtgaatct
      481 ctccgaggcg ctgaatgccg tgcccgggat cgtcgtgcag aaccgctaca actacgcgca
      541 ggacctgcag atctcatcgc gtggattcgg cgcgcgcgcg agcttcggcg tgcgcggcat
      601 ccggctcatt gccgacggca tcccggcgac gatgcccgac ggccagggac aggccgccac
      661 ttttaacctc gacaccgcgg agcgcatcga agtccttcgc gggcccttct cctcgatgta
      721 cgggaattcg tccggcggcg tgattcagat attcaccgcc gacgcgccgc gcgatccgac
      781 agtgacgacg agctattacc gcggcacgtt cgacacgacc aaaacgaata cgcagtacgg
      841 cgcgcagcac gggccgctca atgcgaacgt cgcgtacagc ctcttcgaga ccggcggctt
      901 ccgcgatcac agcgcggcgc ggcgtgagca cgggcacgcg aagctcaagt tcgacggcgg
      961 cagcgccggc acgctgacgg tgctcgccga tgcgctccat cagccggaca cgcaggaccc
     1021 gctcggcctc acgcgcgcgc aggttcagca ggatcggcgg caggccggga ccaacgcgct
     1081 tgctttcaac acgagaaaaa gcgtcgcaca gagccaggtc ggcctcgtgc acgatttgaa
     1141 gctcacgagc cacgacacac tgcagacgcg cttctacgtc ggcgatcgcc aggtcacgca
     1201 gtaccttggg attcccatcg cgacgcaggc ggcgccgacg cattcgggcg gcgtcgtcga
     1261 cctggatcgc ggctactggg gcggcggctt gcgctggagc cgcagcaaca cgcttctcgg
     1321 cgggccgttc gacgtgcacg tcggcgccga ttacgagcgc atggccgagg ggcgccgcgg
     1381 ttacatcaac agcttcggca accagggcaa tctgaagcgc gacgagcacg acaccgtcta
     1441 caccgccgac ttctatttgc agagcgaatg gcgctttcac gagcgctggc tgctcatggc
     1501 gggcgtccgc cgcagcaacg tgcatttcaa ctcgcgcgac ttcttcgtca ccgccgccaa
     1561 cggcgacgac agcggcggca tcgacttttc gaagacgctg ccggtcgcgg gcgtcacgtt
     1621 cgatgtcgcg ccgcgcgtga agctctacgc gaacctcggc aaaggtttcg agacgccgac
     1681 gttcgccgag ctcgcttatc gccccgacgg caccacaggc ctcaacttcg cgctgcggcc
     1741 ttcggtcagc accaatcgcg agatcggcag caagttcgag ctgactccga cgagccgcgc
     1801 aacggtcgcg ttgttccgga tcgacgtcgc cgacgagatc gtcgtggcga gtaacaccgg
     1861 cggacgaacg acgttcaaga acgcggcgca cacgcggcgc gaaggcgtcg agctcggctt
     1921 gcagggccgg tggctcgatc aattcgaagc gtcggtcgcc tacacgcgca tccgcgcgcg
     1981 gtttaccgac gcattcacga cctccaccgg cacgcctgca accatcgtca ccgtgaatcc
     2041 cggcagccgc ctccccggcg ttccgcagag cacgctttat ggagagctcg tgtggcgtca
     2101 cgcgcgaagc ggcttccatg ccgcggcgga ggtgcgccac aacgggagcg tgtacgtcga
     2161 cgaccagaac agcgacgccg cgtcctccta cacgatcggc aacgtgcgct tcggcttcga
     2221 gcagcaggga cgcggctggc gcctctccga gttcgtgcgg gtcgacaatg tcacgaaccg
     2281 cgaatacgtc ggctcggtga tcgtcgccga ggctaacgga cgtttcttcg agccggcgcc
     2341 aggccgcaca tggctcgtgg gcgtaagcgc gtcactcacc ttccgttaaa atcgacggtc
     2401 tcatgctgac cgtcaccatc aacggcgaaa gccgccagtt gcctgccgcc atgcccgtcc
     2461 aagccctgat cgaagagctc ggcctcacgg gcaagcgcat cgctgtcgag cgcaacggcg
     2521 aaatcgttcc gcgcagcgag ttcgccgcgc gttccattct cgacggcgac aagctcgaga
     2581 tcgtcgtcgc agtaggaggc ggctgatggg aattctgatg aaagcgttca gcgccgcggc
     2641 gctgacggtt atgtttgcgg ccgccgcagc cgcgcaggag ttcccgagcc gtccggtacg
     2701 cttcgtcgtt ccgctcacgg ctgcctccgg tgcggacatc gccggccgca tcgtcggccg
     2761 gcagcttcag gagatgtgga agcagacggt cctcgtcgac aatcgccc
//
LOCUS       DATLEE010000501         2656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5784646, whole genome shotgun sequence.
ACCESSION   DATLEE010000501 DATLEE010000000
VERSION     DATLEE010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5784646"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..2264)
                     /locus_tag="VM692_10870"
     CDS             complement(42..2264)
                     /locus_tag="VM692_10870"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HUQ52713.1"
                     /translation="MNNAKIIYTLTDEAPALATYSLLPIVQAFTKAAGCTVETRDISL
                     AGRILAQFPSSLKKDQVVGDDLTELGEIAKTPEANIIKLPNISASVPQLQSAIKELQQ
                     HGYAIPDYPEEPRTEAEKEIKAKYAKVLGSAVNPVLREGNSDRRVAASVKQYAKKHPH
                     SMGRWSKESKTHVATMAGGDFYSSEQSAVVQRAGKLKIELTSNRGEKTALKDGISVKE
                     GDVLAASSMNRRALRDFYAKQIADAKAKGLLLSLHLKATMMKVSDPIMFGHAVEVYYQ
                     DVLTKHADTLKKLGVEPNNGIGDLYTKIKSLPDAERSVIETDLAAVYQARPELAMVDS
                     AKGITNLHVPSDVIIDASMPAAIRASGQMWGPDGKLHDTKAMIPDRCYSGIYQEVIDD
                     CRKNGAFDVPTMGNVSNVGLMAQKAEEYGSHDKTFEIATSGTVRVLDADGKALMEHRV
                     EQGDIWRMCQTRDLAVRDWVKLAVGRARATGQPAIFWLDENRAYDRNVIDKVRRYLRD
                     HETSGLDIRIMSPVNAIRATLKRVRDGVDTISVTGNVLRDYLTDLFPILELGTSAKML
                     SIVPLLAGGGLYETGAGGSAPKHVHQFVQENYLRWDSLGEFLALAVSIEDYGTKTGKT
                     AAIVMAEALDAANGRFLESDKSPSRAVGGIDNRGSHFYLAMYWARALAEQTKDAALRA
                     RFVKVADELESNEQKIVGELNAVQGKPVDIGGYYHPNPQLTAKAMRPSDTLNAILARL
                     "
     gene            complement(2361..>2656)
                     /locus_tag="VM692_10875"
     CDS             complement(2361..>2656)
                     /locus_tag="VM692_10875"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642116.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HUQ52714.1"
                     /translation="KQLDALKPDQRVMLKLTIPDKDNLYADLIKHKNVLRVVALSGGY
                     SRDDANKRLARNNGMTASFSRALAEGLTAQQSEKEFNAALDASVESIYRASIT"
BASE COUNT          390 a          852 c          869 g          545 t
ORIGIN      
        1 accgtttcgc aaaattactg cgtagtcgaa ccgctgcgca gtcagagccg cgccaaaatc
       61 gcgttcagcg tgtcgctcgg gcgcatggcc ttcgccgtca actgcgggtt cggatgatag
      121 taaccgccga tgtcgacggg cttcccctgt accgcgttga gctcgccgac gatcttttgt
      181 tcgttgctct cgagctcgtc ggcgaccttg acgaaccgcg cgcgcagcgc cgcgtctttc
      241 gtctgctcgg ccaaggctcg cgcccagtac atcgccagat aaaaatggct gccgcggttg
      301 tcgatgccgc cgacggcgcg cgacggcgat ttgtcgctct cgaggaaccg gccgttggcg
      361 gcgtcgagag cctcggccat gacgatggcc gccgtcttgc cggtcttcgt cccgtagtcc
      421 tcgatcgaga ccgccagcgc caggaactcg cccagcgaat cccagcgcag gtagttctct
      481 tgaacgaact ggtggacgtg cttcggagcg gagccgccgg cgcccgtctc gtacagcccg
      541 ccgcccgcga gcagcggcac gatcgacagc atcttggcgc tcgtgccgag ctccaggatc
      601 gggaacaggt cggtcaggta gtcgcgcagc acgttgccgg tcacggagat ggtatcgacg
      661 ccgtcgcgga cgcgcttcag cgtcgcgcgg atcgcgttga cgggcgacat gatccggatg
      721 tcgagcccgc tcgtctcatg gtcgcgcagg tagcggcgga ccttgtcgat gacgttgcgg
      781 tcgtaggcgc ggttctcgtc gagccagaag atcgcgggct gacccgtggc gcgggcccgg
      841 cccacggcca gcttgaccca atcgcgtacg gctaagtccc tcgtctggca catgcgccag
      901 atgtcgccct gctcgacgcg gtgctccatg agcgctttgc cgtcggcgtc gagcacgcgc
      961 acggtgccgc tcgtcgcgat ctcgaacgtc ttgtcgtgcg agccgtattc ctccgccttt
     1021 tgcgccatca ggccgacgtt cgagacgttg cccatcgtcg ggacgtcgaa cgcgccgttc
     1081 ttgcggcaat cgtcgatgac ttcttggtag atgccggagt agcagcggtc cgggatcatg
     1141 gccttggtgt cgtgcagctt gccgtccggg ccccacatct ggcccgaggc gcggatcgcg
     1201 gccggcatgg aggcgtcgat gatgacgtcg ctcggcacgt gcaggttcgt gatgcccttc
     1261 gccgagtcga ccatcgcgag ctccggccga gcctgataaa cggccgcgag atcggtttcg
     1321 atgacggaac gctcggcatc cggcaacgac ttgatcttcg tgtacagatc gccgatgccg
     1381 ttgttgggct cgacgccgag cttcttcagc gtgtcggcgt gcttcgtgag cacgtcctgg
     1441 taatagacct cgacggcgtg gccgaacatg atcggatcgg acaccttcat catcgtggct
     1501 ttgaggtgca gcgacagcag caggcctttg gccttggcgt ccgcgatctg cttcgcataa
     1561 aagtcgcgca gcgcgcgccg attcatcgac gaggccgcca gcacgtcgcc ttccttgacg
     1621 ctgatgccgt ccttgagggc ggtcttctcg ccgcgattgc tagtgagctc gatcttgagc
     1681 ttgccggccc tctggacgac ggccgactgc tcgctcgagt agaagtcgcc gccggccatc
     1741 gtggcgacgt gggtcttcga ttccttgctc cagcgcccca tcgagtgcgg gtgcttcttc
     1801 gcgtactgct tgaccgaggc cgccacgcgg cggtccgagt tgccttcgcg cagcacggga
     1861 ttgacggcgc tgccgagcac cttcgcgtac ttcgccttga tctccttctc ggcctccgtg
     1921 cgcggctcct cgggatagtc gggaatcgca tagccatgct gctgcagctc cttgatcgca
     1981 ctttgcagct gtggcacgga cgcgctgatg ttcggcagct tgatgatgtt cgcctcgggc
     2041 gtcttggcga tctcgccgag ctcggtcaga tcgtcgccga cgacctggtc ctttttgagg
     2101 ctgctcggga actgcgcgag gatgcgcccc gcaagcgaga tgtcgcgcgt ctcgacagtg
     2161 cagccggccg ctttcgtgaa cgcttgcacg atcggcagca gcgaataggt cgcgagcgcc
     2221 ggcgcctcgt ccgtcagcgt gtagatgatc ttggcgttgt tcatgggtcc ttacatccga
     2281 aaggcgcggc cacctacgcg gaggcgccgc ggaattgcgc ctcgccgctc ctgacgaggc
     2341 cgaagcgagc gcggcagcgc tcaggtgatc gatgcgcggt agatgctctc gaccgaggca
     2401 tcgagcgctg cgttgaactc cttctccgac tgctgggccg tgaggccttc tgcgagggcg
     2461 cgcgagaagc tcgccgtcat gccgttgttt cgcgcaagac gcttgttggc gtcatcgcgc
     2521 gaatatccgc ccgaaagggc cacgacgcgc agcacgttct tgtgcttgat gaggtcggcg
     2581 tagaggttgt ctttgtcggg aatcgtgagc ttcagcatca cgcgttggtc gggcttcagc
     2641 gcgtccagct gcttcg
//
LOCUS       DATLEE010000502         3895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2807601, whole genome shotgun sequence.
ACCESSION   DATLEE010000502 DATLEE010000000
VERSION     DATLEE010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2807601"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(439..1593)
                     /locus_tag="VM692_10880"
     CDS             complement(439..1593)
                     /locus_tag="VM692_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52715.1"
                     /translation="MKNNKRDCLGAVAGVALLLGVDSASAGLVHIQVQQRAGMEFGAG
                     YTVGRGSDCFVVTPFHVVQFASADSITITDAKGSSAKARLLKGSEEFDAGLLQVAVPH
                     TLDCPADWSDGANASTAIGAAPFLVARKVDDNGRVMQTRFFASSTSREQVELQPFGPN
                     DELREGDSGSTLYAGDQVVGLVVSVDTESRVALALTQSQIHGLFGGDVLPGARRSALM
                     QPFKFRNAEEPYATVAARTYLTTAAGLDLVNAAADGSAPPGAAFVVVGQIVDLRATRK
                     ANPDYKPPEKAPTDESLGRQLFRNLERRVSSEVDEALERNSEAQYLQVFNVDVQVEIT
                     KVADNSKVVNLERRMYEMPETGVTQPDMQKTAVESAVRETLELTLQKYPL"
     gene            complement(1574..2671)
                     /locus_tag="VM692_10885"
     CDS             complement(1574..2671)
                     /locus_tag="VM692_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52716.1"
                     /translation="MADTPPSPEPAAAAPLQDPPPKPAVVGNRRRAAARFAQRSFDAL
                     LNWPGGRWPVPTVTSLFVISLLVVAAVEWLPKELSDVTLSVRAQTEVLELELQPARTY
                     VWWLPGGSYSLLTANGETDCEHRSRFDVVCAYAAPTAITVKNGATVRFELTTAEGGAM
                     PRFLLALTPRADSEFEVRSAADEVLVATSELVTFESQPVAQWRIPLIVERVQIGESLS
                     DSVAASDALGTIARQPIMMAGDVRMFARAFGFGADERYQIKEERFDPADVVQIPADAD
                     EKGLLLGLLSLDAAQQREFDLTLHSDLAEVFVRRLGAEHRIGVSMWKIVSQLPMWLAL
                     WVVWVSLIVVANYYSDRLGELRGHKSEEQQT"
     gene            complement(2678..3448)
                     /locus_tag="VM692_10890"
     CDS             complement(2678..3448)
                     /locus_tag="VM692_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52717.1"
                     /translation="MNVVRFRRDGKEVPSAAWLLCCAAALSASSAAAQVARLAPVGKP
                     VEVVDSSERISGNAAAGVVLVQEGDAIGSDRLWGYLSEPVPAQLKLRISSIDGRYYAE
                     VDYTTASQQAGWVALDLPLSQFSFLEKNYKDPLSEIAALLAAADEPRFYPLRWGSPHR
                     AAAAPPQPPKDTDPLRVYVNTERAQAFVVVDGGPAYCRDASAVSGFKFNAICDVALGD
                     VRNPVADAGASVVSGIEVFRRSGVRTLAPLALDVLIRY"
     gene            complement(3578..>3895)
                     /locus_tag="VM692_10895"
     CDS             complement(3578..>3895)
                     /locus_tag="VM692_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52718.1"
                     /translation="TNAQANAGVTWLVDGVAGGNATVGTITPNGSYIAPAAVPSPAAV
                     TVTATSLEDPTKTATAELTITAPQPPPAASSGGGGGAGLDLLLLLCALTLAWPAQKHR
                     RAD"
BASE COUNT          642 a         1307 c         1320 g          626 t
ORIGIN      
        1 gacggcaatc cgctcgacgt cggccgcaaa caacgcacgg tgtcgacgtc gctaaagcgc
       61 gctctctatg cgcgcgaccg cggctgcacg ttccccggtt gcgatcgcac gcgatatctc
      121 gacggccatc acctgaggca ctggctcaac ggtggggaga cgaagcccga caacatgacg
      181 ttgctgtgca cgcatcacca tgggctgctg caccgtggcg cgttcgacat cgtgaaggac
      241 gccgacggca tgctgcgatt cgtgacggcc gagggccgca cgattccacg cgacggctac
      301 cggctagagg acttcgtcga tgacgacagc ggctacgccg atgacgcgcc acgtggcgcg
      361 ttttactcca cgtccccact cggagaaggc gaacgcggag gtgcgcgagc ccgctgcggt
      421 ctaccgtttg ttcgctccct agagcgggta cttctgcagc gtcagctcga gcgtttcgcg
      481 caccgcgctc tcgacggccg tcttctgcat atcgggctgc gtcacgccgg tctccggcat
      541 ctcgtacatg cgccgctcga gattgacgac tttgctgttg tcggcgacct tcgtgatctc
      601 gacttgcacg tcgacgttga agacttggag gtactgcgct tcgctgttgc gctcgagcgc
      661 ttcgtcgacc tcgctcgaga cgcgccgctc gagattgcgg aacagctggc ggccgaggct
      721 ctcgtcggtt ggcgccttct cgggcggctt gtagtcaggg ttcgctttgc gcgtcgctcg
      781 cagatcgacg atttggccga cgacgacgaa cgcggcgccg ggcggcgcgc tgccgtcggc
      841 ggcggcgttc acgaggtcga gcccggctgc agtcgtgagg tacgtccgag cggcaaccgt
      901 cgcgtacggc tcctcggcgt tgcggaactt gaacggctgc atgagagccg agcggcgcgc
      961 gcccggcagc acgtcgccgc cgaacaggcc gtgaatctgg ctttgcgtga gcgcaagtgc
     1021 gacgcgcgac tcggtgtcga cggacacgac aagaccgacc acctgatcgc cggcatagag
     1081 cgtgctgccg ctgtcgcctt cgcgcagctc gtcgttgggg ccgaacggtt gcagctcgac
     1141 ctgctcgcgc gacgtgctcg aagcgaagaa ccgcgtctgc atgacgcggc cgttgtcgtc
     1201 gaccttgcgc gccactagaa acggcgcggc gccgatcgcc gtgctcgcgt tcgcgccgtc
     1261 ggaccaatcc gcgggacagt cgagcgtgtg cggcacggcg acttgcagca gtccggcgtc
     1321 gaactcctcg ctgcccttca gcaaccgcgc cttggcgctg ctgcctttcg cgtcggtgat
     1381 cgtgatggaa tcggccgagg cgaactgcac gacgtgaaac ggcgtgacga cgaagcagtc
     1441 ggagccgcgg ccgacggtat atccggcgcc gaactccatg ccggcgcgtt gctgcacttg
     1501 gatgtggacg aggccggccg acgccgagtc gacgccgagc agcagagcga cgcctgcgac
     1561 agcgccgaga cagtcacgtt tgttgttctt cactcttgtg accccgcagt tcaccgagac
     1621 gatcggaata gtaattcgcc acgacgatca gcgacaccca gacgacccac agagcgagcc
     1681 acatgggcag ctgcgagacg attttccaca tcgagacgcc gatgcggtgc tcggccccga
     1741 gccgccgtac gaacacctcg gcaagatcgg agtgcagcgt caggtcgaac tcgcgctgct
     1801 gcgctgcgtc gagcgacagc aacccgagca acaacccctt ctcgtccgca tcggccggaa
     1861 tttgcacgac gtcggccgga tcgaagcgct cctcttttat ctggtagcgc tcgtcggcgc
     1921 cgaagccaaa ggcgcgcgcg aacatccgca cgtcgccggc catcatgatc ggctggcgcg
     1981 cgatcgtgcc gagcgcgtcc gaggccgcca cgctgtctga caacgattcg ccgatctgca
     2041 cgcgttcgac gatcaacgga atgcgccatt gcgccacggg ctgcgactcg aacgtcacga
     2101 gctcggaggt cgccacgagc acctcgtcgg cggcgctgcg cacctcgaac tccgagtcgg
     2161 cgcgtggcgt caacgcgagc aagaagcgcg gcatcgcacc gccctcggcc gtcgtcagct
     2221 cgaagcgaac cgtggcgccg ttcttgaccg tgatggccgt cggcgccgcg taggcgcaaa
     2281 cgacgtcgaa gcggctgcgg tgctcgcaat ccgtctcgcc gttcgccgtg agcaatgagt
     2341 agctgccgcc gggcagccac cagacgtaag tgcgcgcggg ttggagctcg agctcgagca
     2401 cttccgtttg cgcgcgcacg ctgagcgtca cgtcgctcaa ctctttcggc agccactcga
     2461 cggccgcaac gacgagcagc gaaatgacga acaagctcgt gacagtgggc acgggccagc
     2521 gaccgccggg ccaattcaac agcgcgtcga acgatcgttg cgcgaaacgc gccgcagctc
     2581 gacggcggtt gccgacgaca gccggcttag gcggcgggtc ctgcagcggc gcagcggctg
     2641 cgggctcggg gctcggcggc gtgtcggcca tgaggtgtca gtagcggatc agcacatcca
     2701 acgcgagtgg tgccaacgtg cgcacgccgg agcggcggaa cacctcgatg ccgctgacga
     2761 cgctggcgcc ggcatcggcc acggggttac gcacgtcgcc gagcgccaca tcgcagatcg
     2821 cgttgaattt gaaacccgac acggccgagg cgtcgcggca gtaggccggt ccgccgtcga
     2881 cgacgacgaa tgcctgcgcg cgctcagtgt tgacgtagac gcgcagcggg tccgtgtcct
     2941 tcggcggctg cggcggcgcg gccgccgcgc gatgcggcga gccccaacgc agcggataga
     3001 agcgcggctc gtcggcggcg gcgagcagcg ccgcgatctc ggacagcggg tccttgtaat
     3061 tcttctcgag gaacgagaac tgactcagcg gcaaatcgag cgcgacccag ccggcctgtt
     3121 gcgacgctgt cgtgtagtcg acctcggcgt agtagcgccc gtcgatgctg ctgatccgca
     3181 gcttgagctg cgcgggcacg ggctcggaca gataacccca cagccggtcg ctgccgattg
     3241 cgtcgccttc ctgcacgagc acgacaccgg cggcggcatt gccgctgatg cgctcggagg
     3301 aatcgacaac ctcgacgggc ttgcctacgg gagcgaggcg tgcaacttgc gcagccgccg
     3361 acgacgccga tagcgccgcg gcgcagcaga gcagccacgc agcggagggg acttccttgc
     3421 cgtcgcggcg gaatcgcacg acgttcatcg gttcactctt attgtcggtg ttatggcaac
     3481 cgttcgctgc tacgcgtcgg cgcagcggca cgatcatagc accgcgtccg gagagacgag
     3541 cggccggtcg cggcacggcg ccgaagttgc gagggagcta gtcggcgcgc cggtgcttct
     3601 gcgccggcca cgcgagcgtc agcgcgcata acagcaaaag caggtcgagt cccgcgccgc
     3661 cgcccccgcc cgatgacgcc gccggcggcg gttgtggcgc cgtgatcgtg agctccgccg
     3721 ttgcggtttt ggtcggatcc tcgagggacg tcgccgttac cgtcactgcc gcgggcgatg
     3781 gcaccgcggc cggcgcgatg tacgaaccgt tgggcgtgat cgtgccgacg gtggcgttgc
     3841 caccggcaac cccgtcgacg agccaggtca cacccgcatt cgcttgcgcg ttcgt
//
LOCUS       DATLEE010000503         2709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6996275, whole genome shotgun sequence.
ACCESSION   DATLEE010000503 DATLEE010000000
VERSION     DATLEE010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6996275"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VM692_10900"
     CDS             <1..433
                     /locus_tag="VM692_10900"
                     /inference="COORDINATES: protein motif:HMM:NF022467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HUQ52719.1"
                     /translation="DHHELFVVDAQGRERAFNMVDMDIAVREGNTVSVIWVVPQDVES
                     GPHVQVVNHNTGDTTTVSPIRVITWFFRKKQMWIATIAGTILGFIIFWPIGLAMMIAP
                     YLYFKRRAINAIKGMFTTREFTQLQSQLAQVKPMSSPAAAA"
     gene            524..1111
                     /locus_tag="VM692_10905"
     CDS             524..1111
                     /locus_tag="VM692_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52720.1"
                     /translation="MPKFRDAVVAVGGMVLGTLVGTGVQQVLARTGVLGPGVDSLIAE
                     QQANFVEVNSKLDTLRQVSNDPAAQRALTELRTLLTQQGALATQTEQQLKLLSGEVAA
                     SKERELAERGMSGGADFWLKLGESMNLRSPENVFALRGYAQGLAIVNLGGAAAKRLAV
                     GDAIDFKVGEDACKVFYKQATPRADGRIGFDVDCK"
     gene            complement(1115..1672)
                     /locus_tag="VM692_10910"
     CDS             complement(1115..1672)
                     /locus_tag="VM692_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HUQ52721.1"
                     /translation="MSTRTSRIVGICLSWAAAMSIQHSVAGQENAAAAPAPKLVFAFE
                     LRAQVGPPAELGEVTHGRRRIVPILGGTVKGPELNAKVVPGGADWQMIQADGFSELDT
                     RYTLETDRGELVYVQNAGIRHAAPDVMKKLLAGEIVDPKLVYFRTVPKFETSAPNLQW
                     LARSVFVGVGERFPSEVVVRFYRLE"
     gene            complement(1767..>2709)
                     /locus_tag="VM692_10915"
     CDS             complement(1767..>2709)
                     /locus_tag="VM692_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ52722.1"
                     /translation="FNLQGGGGAVFDPLLPVTAPQVQITVTSGGNPSLSPEEADTKTA
                     GFVYQKGGLQLSVDWYDIQLSEAVGQVGAQNIVNGCYQQGRQNLCAFVQRDANGVIGN
                     VRDVFQNINEARVRGIDYELLLNAEPNFAKNQSEALTFRFLAGRLLEESTTLLGGPTP
                     DFRDVAGRWYEPDLKLLASVRYSIGAWGVSFQQRYLPETRLDGGLNPVQATGVDGPNW
                     VEWQPGMTIGALPTGAFTIDNNTVQSKSYTDVVLAYDGEMRSGHTWEASLAITNLLDE
                     DPVVIPSFDTRFSSQLTTGNNFDIYGRRYLASFRYRF"
BASE COUNT          473 a          881 c          887 g          468 t
ORIGIN      
        1 cgatcaccac gagctgttcg tcgtcgacgc acagggccgc gagcgcgcgt tcaacatggt
       61 cgacatggac atcgccgtac gcgaaggcaa caccgtgagc gtcatctggg tggtgcccca
      121 ggatgtcgag tcgggcccgc acgtgcaggt cgtcaatcac aacaccggcg ataccacgac
      181 ggtgagcccg attcgcgtca tcacctggtt cttccgcaag aagcagatgt ggattgcgac
      241 gatcgccggc acgatcctgg gcttcattat cttctggccc atagggctcg cgatgatgat
      301 cgcgccgtat ctctacttca aacgccgagc aatcaacgcg atcaaaggca tgttcacgac
      361 gcgcgagttt acgcagctgc agtcgcagct cgcgcaggtg aagccgatgt cttcgcccgc
      421 cgctgcggcc taaggcagcg acgtgaccac cgtggaaggg ccggccgcaa gtccggccca
      481 cccaccttta ccggccgcgc cacgttttaa agcgccgttc aagttgccga agtttcgcga
      541 cgccgtagtc gcggtcggcg gcatggtgct cggcacgctc gtcggcaccg gcgtgcagca
      601 ggtgctggcg cgcacgggtg tgctcggccc cggcgtcgac tcattgatcg ccgagcagca
      661 agcgaacttc gtcgaggtca actcgaagct cgacacgttg cggcaagtgt cgaacgaccc
      721 cgccgcgcag cgcgcgttga cggagctgcg cacgttgctg acgcagcagg gcgcgctagc
      781 cacgcagacg gagcagcaac tgaagctgtt gtccggcgag gtcgccgcga gcaaggaacg
      841 cgagctcgcc gagcgcggca tgtccggcgg cgcggatttc tggctcaagc tcggcgagag
      901 catgaatctg cgttcgcccg agaacgtttt tgcgttgcgc gggtatgcgc agggtctcgc
      961 catcgtcaac ctgggcggcg cggcggcgaa acgcctcgcc gtcggcgatg cgatcgactt
     1021 caaggtcggc gaggacgcgt gcaaggtctt ctacaagcag gcgacgccgc gcgcggacgg
     1081 ccgaatcggc ttcgacgtcg actgtaagta gccgctattc gaggcggtaa aaccgcacga
     1141 cgacctcgct cggaaagcgc tcgccgacgc cgacgaacac gcttcgggcc agccattgca
     1201 gattcggcgc ggacgtctcg aacttcggaa ccgtgcggaa gtagacgagc ttgggatcga
     1261 cgatctcgcc ggccagtagc ttcttcatga cgtcgggcgc cgcgtggcga atgccggcgt
     1321 tttgcacgta gacgagctcg ccgcgatcgg tctcgagcgt gtagcgcgtg tctagctcgg
     1381 aaaacccgtc ggcttgaatc atctgccagt ccgcgccgcc cggcacgacc ttggcgttca
     1441 gctcggggcc tttcacggtg ccgccgagaa tgggcacgat gcgccgccgg ccgtgggtga
     1501 cctcgccgag ctcggcgggc ggcccgacct gcgcgcgcag ctcgaaggca aacacgagct
     1561 tcggcgccgg cgcagccgcc gcgttctctt gtccggcaac ggaatgctga atgctcatgg
     1621 cggcggccca actcaagcag ataccgacga tacgtgacgt tcgcgtgctc atgcgcgcga
     1681 tgctagcgcg attgcagcac gcgtgcagca gaaggtgtca gcgcggcgcg cgcatcggcg
     1741 gggcctgagc cccgccgacc gcgacgctag aagcgatagc ggaagctggc gagataacga
     1801 cgaccgtaga tgtcgaagtt gttgccggtc gtcagctgag agctgaaccg cgtgtcgaac
     1861 gacggaatca cgacggggtc ctcgtcgagc aggttcgtga tcgcgagcga cgcttcccag
     1921 gtgtgaccgc tgcgcatctc gccgtcgtac gccaacacga cgtccgtgta cgacttcgac
     1981 tgcacggtgt tgttgtcgat cgtgaacgca cccgtcggta atgcaccgat ggtcatgccg
     2041 ggctgccact cgacccagtt cggcccgtcg acaccggttg cctgcacggg gttcaacccg
     2101 ccgtcgagac gcgtctcggg caagtagcgc tgctggaagc tcacgcccca cgcaccgatc
     2161 gagtaccgga cgctcgccag cagcttgagg tcgggctcgt accaacggcc cgcgacgtcg
     2221 cggaagtcgg gtgtcggccc gccgagcaac gtcgtgctct cctcgagcaa gcggcccgcg
     2281 aggaagcgga acgtcagcgc ttcgctttga ttcttggcga agttcggctc cgcgttcagc
     2341 aacagctcgt aatcgatccc tctgacgcgc gcctcgttaa tgttctggaa cacgtcgcgc
     2401 acgttgccga tcacgccgtt cgcatcgcgc tgcacgaacg cgcagaggtt ttgtcggccc
     2461 tgctggtagc agccgttgac gatgttctgc gcgccgactt ggccgaccgc ttcgctcaac
     2521 tggatgtcgt accagtcgac ggacagctgc aagccgccct tctgatagac gaagcccgcc
     2581 gtcttcgtgt cggcttcctc ggggctcaag ctcgggttgc cgccgctcgt gaccgtgatc
     2641 tgcacttgcg gggccgtgac cggcaatagc gggtcgaaaa ccgcgccgcc gccgccctgg
     2701 aggttgaac
//
LOCUS       DATLEE010000504         2400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3281112, whole genome shotgun sequence.
ACCESSION   DATLEE010000504 DATLEE010000000
VERSION     DATLEE010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3281112"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /gene="map"
                     /locus_tag="VM692_10920"
     CDS             <1..506
                     /gene="map"
                     /locus_tag="VM692_10920"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HUQ52723.1"
                     /translation="GDIINMDITVIHDGYHGDTSAMFHVGTPQPHAERLVNITKECLY
                     RGIAEVRPGARLGDIGHAIQHHAEANYYSVVREYCGHGIGRVFHEDPQVLHYGQPGSR
                     EELVPGMTITVEPMVNAGKREVKLLSDGWTVVTKDHSLSAQWEHTVLVTPTGFEVLTL
                     SPADRGT"
     gene            512..>2400
                     /gene="glnD"
                     /locus_tag="VM692_10925"
     CDS             512..>2400
                     /gene="glnD"
                     /locus_tag="VM692_10925"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HUQ52724.1"
                     /translation="MRNPEDTSAPLIDRGALEQALRGDVRAVKAFRDALKTANDQLGE
                     RFRRNEAIETLVAGRAQVVDEVILASWVHFAQKVLESADLVAVGGYGRGELHPQSDID
                     LLVLLESASTTTDDAIGRFLTFLWDIGLEVGHSVRTLDDCEAQAKSDVTVLTTLMETR
                     LLHGPGTLFAELPGRIGTDRMWNSAEFFHAKLAEQKARHARYHDTAYNLEPNVKGSPG
                     GLRDIQMIGWLAQRHLGTRTLAELVPHKYLTEGQLRILTQGRAFLWRVRFGLHLLTGR
                     REDRLLFDHQVKLSQMLGYEDASFTLAVEQLMQRYYRTVMELSRLNEMLLQLFEEAIL
                     LDKDAPPSPLGPRFQSRNGYLEVTDDQVFAREPSALLEMFLLLEQNLDLRGVNARTIA
                     LVKQHLWLIDEEFRQNPRHHRLFLDILRAPQGVTRTLKRMNTYGVLGLYIPAFGRVVG
                     RMQYDLFHAYTVDAHTLFVVENLRRFALPRFDKEFPSCSAIMQRLEKPEVAYLAGLFH
                     DIAKGRGGDHSELGSVDAESFCLEHGMSRYDARLVAWLVKQHLLLSLTAQKKDLNDPN
                     VVNEFARTVGDQTHLDYLYVLTVADVRGTNPKIWNSWKESLFAELYQSTRRALRRGVS
                     NPIDK"
BASE COUNT          443 a          809 c          752 g          396 t
ORIGIN      
        1 acggcgacat catcaatatg gatatcaccg tgatccacga cggctatcac ggcgatacca
       61 gcgccatgtt tcatgtcggc actccgcagc cgcatgccga gcggctggtc aacatcacca
      121 aggaatgcct gtatcgcggc atcgccgaag tgcgacccgg cgcgcggctt ggcgatatcg
      181 gccacgccat ccaacaccac gccgaagcca attattattc ggtagtgcgc gagtattgcg
      241 gccacggcat cggccgcgta ttccacgagg acccgcaagt gctgcactac ggccaacccg
      301 gcagccgcga ggagctcgtg cccggcatga cgatcaccgt cgagccgatg gtcaacgccg
      361 gcaagcgcga agtgaagctg ctgtcggacg gctggacggt cgtcacgaag gaccactcgt
      421 tgtccgcgca atgggagcac acggtgctcg tcacgcccac gggcttcgag gtgctgacct
      481 tgagccctgc cgatcgcggc acctgacgag catgcgcaat cccgaggaca catcggcgcc
      541 gctgatcgac cgcggcgcgc tcgaacaagc gctgcgcggc gacgtcaggg ccgtcaaagc
      601 gttccgcgac gcgctgaaga ccgccaacga ccagctcggc gagcgctttc ggcgcaacga
      661 agccatcgaa acgctcgtgg cgggccgcgc ccaggtcgtc gacgaggtca tcctagcgag
      721 ctgggtccac ttcgcgcaga aagtcctcga gtcggccgat ctcgtggccg tcggcggcta
      781 cggccgcggc gagctgcatc cgcagtccga catcgatctg ctcgtgctgc tcgaaagcgc
      841 gtcgactacg accgacgacg cgatcggccg gttcctgact ttcctctggg acatcggcct
      901 cgaggtcggg cacagcgtcc gcacgctcga cgactgtgaa gcgcaagcga agagcgacgt
      961 caccgtgctg acgacgctga tggagacgcg gttgctgcac ggccccggca ctctgttcgc
     1021 ggagttgccc gggcgcatcg gcacggaccg aatgtggaac agcgcggagt tcttccacgc
     1081 gaagctcgcg gagcagaagg cgcggcacgc gcgctatcac gacacggctt acaacctcga
     1141 gcctaacgtc aaaggcagcc ccggcggctt gcgcgacatc cagatgatcg gctggctcgc
     1201 gcagcggcac ttgggcacgc gcacgttggc ggagctcgtc ccgcacaagt acctgaccga
     1261 ggggcagctg cgcattctga cccagggccg cgcgttcctt tggcgcgtcc gcttcgggct
     1321 gcacttgctg acgggtcggc gcgaggatcg cctgctgttc gatcatcagg tcaagctctc
     1381 gcagatgctc ggttacgagg acgcgagctt caccctcgcc gtcgagcagc tgatgcagcg
     1441 gtattaccgc accgtcatgg agctgagccg gctgaacgag atgctgctgc aactgttcga
     1501 ggaagcgatc ctgctcgaca aggacgcgcc tccgagccct ctcgggccgc gcttccagag
     1561 ccgcaacggc tatctcgagg tcacggacga tcaagtgttc gcgcgggaac cgtccgcgct
     1621 gctcgagatg ttcctgttgc tcgagcagaa tctcgacttg cgcggcgtca acgcgcgcac
     1681 gatcgcgctc gtcaagcagc acttgtggct catcgacgag gagttccggc agaacccgcg
     1741 ccaccaccgc ctgtttctcg acatcttgcg cgcgccgcaa ggcgttacgc gcacgctgaa
     1801 gcgcatgaac acgtacggcg tactgggcct gtacattccg gcgttcggcc gcgtcgtcgg
     1861 ccgcatgcaa tacgatctgt ttcacgccta cacggtcgac gcgcacacgc tgttcgtcgt
     1921 cgagaacctg cgaaggttcg cgctaccgcg cttcgacaag gagttcccgt cgtgcagcgc
     1981 catcatgcag cggctcgaga aacccgaggt cgcgtatctc gcggggctgt ttcacgacat
     2041 cgccaaaggc cgcggcggcg atcactcgga gctcggctcc gtcgacgccg agtcgttctg
     2101 cctcgagcac ggcatgagcc gctacgacgc gcgcctcgtc gcctggctcg tgaagcagca
     2161 cttgttgctg tcgctgacgg cgcagaaaaa ggacttgaac gacccgaacg tcgtcaacga
     2221 gttcgcacgc accgtcggcg accagacgca cctcgactat ctctacgtgt tgaccgtcgc
     2281 cgacgtgcgc ggcacgaacc cgaagatctg gaactcgtgg aaagaatccc tgttcgccga
     2341 gctctaccag tccacgcgcc gcgcgctgcg acgcggcgtg tccaacccga tcgacaagga
//
LOCUS       DATLEE010000505         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5787126, whole genome shotgun sequence.
ACCESSION   DATLEE010000505 DATLEE010000000
VERSION     DATLEE010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5787126"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1963
                     /locus_tag="VM692_10930"
     CDS             221..1963
                     /locus_tag="VM692_10930"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HUQ52725.1"
                     /translation="MTTRLKSLRDWVAQVAQLTSPDRIHWCDGTDQEYRKLLDEMLDN
                     GTLVELNQESYPGCYLHRSDPEDVARVEHLTYVCTEKPEDAGPNNNWKAPAEAKALME
                     SCFAGCMRGRTMYVIPYCMGPIDSPYARCGVEITDSAYVVANMYLMTRIGKPALKRIE
                     KDKTFVKGLHSTGDLDPARRYIVHLPEELTIMSFGSGYGGNALLGKKCHALRIASYQA
                     RTEGWLAEHMLIVGVQSPAGDTHYLACAFPSACGKTNLAMLIPPASQQGWKVWTLGDD
                     IAWMHLDSNGQLRAINPEAGYFGVVPGTNPQTNRNAYDMIGKNTIFTNVGVTANNDPW
                     WEEKGSGEPTIDWKGHAYKKANGPAAHPNARFTVSAKQNPSYSKLAEAPEGVPISALV
                     FGGRRRELAPLVYQAKDWAHGVLVGASVASETTAAATGKVGVVRRDPMAMKPFCGYNY
                     GDYWAHWLSFAKRSTSLPKIFHVNWFRRDASGKFLWPGFGDNLRVLRWIIDRCEGRVD
                     AVETPIGFLPRPNDIDLQGLDVSSSTLEQLLAVDPEQWRKEMASIGEYFGEFGERLPA
                     ELKAEHQKVVRALG"
BASE COUNT          413 a          675 c          657 g          330 t
ORIGIN      
        1 cagctacgcc ggcaagcccc gccaacagca cgccatagcg gccgcggcgc gagcttggcg
       61 acgctaagtg cgggagctcg aagtggcgcg gcggtttcgc ggcaaccccc cgacatgctt
      121 ggagttttcg tccgtgtagc ggtgacaccg ttcgcccttt ccgaggccgt gcaggctata
      181 ctagcccgct tccaacagtt cggaactcaa aaccaattcc atgacgaccc gcctcaagag
      241 cctccgtgac tgggttgctc aagtagccca gttgaccagc cccgaccgga ttcattggtg
      301 cgacggcacc gaccaggagt atcgcaagct gctcgacgaa atgctcgaca acggcaccct
      361 cgtcgagctg aatcaggaga gttatccggg ctgctacctc caccggtcgg accccgagga
      421 cgtcgcacga gtcgagcacc tcacgtacgt ttgcaccgaa aaacccgagg acgccggccc
      481 gaacaacaac tggaaggcgc cagcggaagc caaggcgctc atggagtcgt gctttgcggg
      541 ctgcatgcgc ggccgcacga tgtacgtcat cccgtactgc atgggtccga tcgattcgcc
      601 gtacgcacgc tgcggcgtcg agatcacgga cagcgcgtac gtcgtcgcga acatgtacct
      661 gatgacgcgc atcggcaaac cggcgctaaa gcgcatcgag aaggacaaga ccttcgtcaa
      721 aggactgcac tcgacgggcg acctcgatcc ggcgcgccgt tacatcgtgc acctccccga
      781 ggagctcacg atcatgagct tcggctcggg ctacggcggc aacgcattgc tcggcaagaa
      841 atgtcatgcg ctgcgcatcg cgagctatca agcacgaacc gagggctggc ttgccgagca
      901 catgctgatc gtcggcgtgc agagccccgc cggcgatacg cattacctcg cctgcgcgtt
      961 cccctccgcc tgcggcaaga cgaatctcgc gatgctgatc ccgcccgcga gccagcaggg
     1021 ttggaaagtt tggacgctcg gcgacgacat cgcgtggatg cacctcgatt cgaacgggca
     1081 gctgcgcgcg atcaatcccg aggccggcta cttcggcgtc gtaccgggca cgaacccgca
     1141 gacgaaccgc aacgcctacg acatgatcgg caagaacacg atcttcacga acgtcggcgt
     1201 gacggcgaac aacgatccgt ggtgggaaga aaaaggcagc ggcgagccga cgatcgactg
     1261 gaaaggtcac gcctataaaa aagcgaacgg ccccgctgcg cacccgaacg cgcggttcac
     1321 ggtttccgcg aagcagaatc cgagctactc gaagctggcc gaagcccccg agggcgtgcc
     1381 gatctcggcg ctggtattcg gcggtcggcg ccgcgagctc gcgccgctcg tctatcaggc
     1441 gaaggactgg gcgcacggcg tgctagtcgg cgccagcgtg gcctccgaga cgacggctgc
     1501 ggcgacgggc aaggtcggcg tcgtgcgccg tgatccgatg gcgatgaagc cgttctgtgg
     1561 ctataactac ggcgattact gggcgcactg gttgagcttc gcgaaacgct cgacgagctt
     1621 gccgaagata ttccacgtca actggttccg ccgcgacgcg agcggcaaat tcctctggcc
     1681 cgggttcggc gacaacctgc gcgtgctgcg ctggatcatc gaccgctgcg aaggtcgcgt
     1741 cgacgccgtc gaaacgccga tcggcttcct gccaagacct aacgacatcg atttgcaggg
     1801 cctcgacgtg tcgagctcga cgctcgagca attgctcgcc gtcgatcccg agcagtggcg
     1861 caaggaaatg gcgagtatcg gcgaatactt cggggagttt ggcgagcgcc tacccgcgga
     1921 gctgaaggcc gagcatcaga aggtcgtgcg cgcgctcggc tgaacctatt cttcgacgac
     1981 gaagtggacg gcgagcgccg tcccgggctg cgcgactcgc ccgagcccga tgacgccctc
     2041 gcccgtgagc tgcgtgctgt cgcgccgcac gaaga
//
LOCUS       DATLEE010000506         2193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5788658, whole genome shotgun sequence.
ACCESSION   DATLEE010000506 DATLEE010000000
VERSION     DATLEE010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5788658"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VM692_10935"
     CDS             <1..927
                     /locus_tag="VM692_10935"
                     /inference="COORDINATES: protein motif:HMM:NF020876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase domain-containing
                     protein"
                     /protein_id="HUQ52726.1"
                     /translation="NILTRNLIIFGQGAIRCHPFVRAEMDAARNPDREAGLVQFDRLL
                     FKHLGYGLSNAARSLVMAVTFARFSDVPVAGPTRRYYQHINRYSASFALITDAAMFTL
                     GGELKRRELISARLGDILSYLYLASMVLKHYQDQGEPPEDLPLVEWSCRTLLYKTQEQ
                     FHGLLRNFPNRWVAALLRFFIFPRGRTYFAPSDVLGQEIVELIINPTATRERLSEGIY
                     KTVEPGNQIGLLQQAMEMAEQVKPLERRIFDARRAGDIKADDIPGQIDEAEQKGVLTP
                     AEAEAVRAFDRRMLDVTGVDDFDPADLRRHGA"
     gene            941..2041
                     /locus_tag="VM692_10940"
     CDS             941..2041
                     /locus_tag="VM692_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52727.1"
                     /translation="MYPRTAASVFALGTVLTAASFAGVARAQSNAIAAIPRVAAATQS
                     RAASCNRACLEGWVDRYFAAVIADDPSAVPLAPNVRFTENGQVLPVGEGLWKSMKSAG
                     NYRLPVADPEAGQIALITTVVEDHADPARGVGALLALRLKIVDGRIAEIEQIVERNEM
                     TAQRVEALGTPRAAFSTPIPANQRMSRADLITTANKYFSGMQRNDGKGDYPFGDRCDR
                     IENGGRSTNAPTPAGQTRPDPKTATGYSGQWSCREQFESGLLHFVSRIRDRRYVAVDQ
                     ERGVVAAFAFFDHMAGSTRNFTRPDGQPTTAGPAQPWTWEILELFKIENGQLAEIEAF
                     LTRPPYGMLSGWSTWEDGMSDKIQEATGYLER"
BASE COUNT          386 a          756 c          704 g          347 t
ORIGIN      
        1 aacattctga cgcgcaactt gatcatcttc gggcagggcg cgattcgctg ccatccgttc
       61 gtgcgcgccg agatggacgc cgcgcgcaat ccggatcgcg aagcggggtt ggtccagttc
      121 gatcgcctct tgttcaagca tttgggctac ggcctatcga acgcggcgcg ctcgctcgtc
      181 atggccgtga cgttcgcgcg tttcagcgac gtacccgttg ccgggccgac gcgccgctac
      241 tatcagcaca tcaaccgcta cagcgcgtcg ttcgcgttga tcacggacgc cgcgatgttc
      301 acgctcggcg gcgagctcaa gcgccgcgag ctgatctcgg cgaggcttgg cgacatcctg
      361 agctacttgt acttggcatc gatggtgctc aagcactatc aggaccaagg cgagccgccc
      421 gaggacttgc ctctggtcga gtggtcgtgc cgcacgctgc tctacaagac gcaagagcag
      481 ttccacgggc ttctgcgcaa cttcccgaat cggtgggtcg ccgcactcct gcgcttcttc
      541 atcttcccgc gcggccgcac ctatttcgcg ccgtccgacg tgctcggcca ggagatcgtc
      601 gagctcatca tcaatccgac ggcgacgcgc gagcgccttt ccgagggcat ttacaagacc
      661 gtcgagccgg gcaatcagat cggcttgttg cagcaagcga tggagatggc cgagcaggtc
      721 aagccgctcg agcggcgcat tttcgacgcg cgccgcgccg gcgacatcaa agccgacgac
      781 attccgggcc aaatcgacga ggccgagcag aaaggcgtgt tgaccccggc cgaggcggaa
      841 gccgtccgcg cgttcgaccg ccgcatgctc gacgtcacgg gtgtcgacga cttcgatccg
      901 gctgacctgc gccgccacgg cgcgtaacag ggaggctccg atgtacccga gaaccgctgc
      961 gagcgtgttc gcgctcggca ccgtattgac cgcggcaagc ttcgccggcg tcgcgcgcgc
     1021 gcagagcaac gccatcgccg ccattccgcg agtcgccgcc gcgacgcaat cgcgcgcggc
     1081 cagctgcaac cgcgcgtgcc tcgaaggctg ggtcgaccgt tacttcgcgg ccgtcatcgc
     1141 cgacgacccg agcgccgtgc cgctcgcgcc gaacgtgcgc ttcaccgaga acggccaagt
     1201 gctgcccgtc ggcgagggcc tctggaagag catgaagtcg gcaggcaact accgtcttcc
     1261 cgtggcggac cccgaagcgg gccagatcgc attgatcacg accgtcgtcg aggaccacgc
     1321 cgatcccgcg cgcggcgtcg gagcgctctt ggcgttgcgg ctcaagatcg tcgacggccg
     1381 catcgcggag atcgagcaga tcgtcgagcg caacgagatg acggcgcagc gcgtggaagc
     1441 gctgggcacg ccgcgcgcgg cgttctcgac gccgattccc gcgaaccagc gcatgtcgcg
     1501 cgccgacttg atcacgaccg cgaacaagta cttctcggga atgcaacgca acgacggcaa
     1561 gggcgactac ccgttcggcg atcgctgcga ccgcatcgag aacggtggcc gctcgacgaa
     1621 tgcgccgacg ccggccggcc agacgcgccc cgatcccaaa acggcgacgg gctactcggg
     1681 ccaatggtcg tgccgcgagc aattcgagag cggactgctc cacttcgtct cgcgcatccg
     1741 cgaccgccgc tacgtcgccg tcgatcagga gcgcggcgtc gtcgcggcat tcgcgttctt
     1801 cgaccacatg gccggcagca cccgcaactt cacccgcccc gacggtcagc cgacgacggc
     1861 cgggcccgcg cagccgtgga cttgggaaat cctggagctc ttcaagatcg agaacggcca
     1921 gctcgcggag atcgaggcgt tcttgacgcg gccgccgtac gggatgctgt ccgggtggag
     1981 cacgtgggaa gacggcatgt cggacaagat ccaagaggcc acggggtatc tcgagcgcta
     2041 agtcgcgcgc cgaagcgccg aaaacccctg tgctagagtg gccgaaaaca agatcccgga
     2101 agccccgccc atgctgaaac acggcgcatt gctcatcgtt tgcggactgc tccacttcgt
     2161 ctcgcgcatc cgcgaccgcc gctacgtcgc cgt
//
LOCUS       DATLEE010000507         4732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9516085, whole genome shotgun sequence.
ACCESSION   DATLEE010000507 DATLEE010000000
VERSION     DATLEE010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9516085"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..828
                     /locus_tag="VM692_10945"
     CDS             238..828
                     /locus_tag="VM692_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUQ52728.1"
                     /translation="MFALNRILVPTNLGDPSRAAINYGVAFARQFGAKLFLLHVLEAK
                     QYDAVLETERVLEVLSPDEAERRASEPTPQEIARNAARHDLGQLLSQEDELATGAEYL
                     LRASGVAGAGEAIVACARELDVELIVMGKHRLGFVEHLLAGSVAEKVMRHAPCPVLIV
                     QHPEGAGAQAHERQFVVADAPPVGDFQGSPAARAMP"
     gene            825..2588
                     /locus_tag="VM692_10950"
     CDS             825..2588
                     /locus_tag="VM692_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HUQ52729.1"
                     /translation="MMWRALLLAGIATMTLGSPAAVKAADEDFQRAFGDDFFDRYWEL
                     SPGVAISYGFYKYADRLIVPDDAARKTSLAQLDRWLAQLARIDPADLSPGVRADRVML
                     ENQLRGERWALTDLRAWQWDPSIYNVAEPFALLLSLDYAPLDERLRAFSKRLTNVPAY
                     YAAAKASVAGPTREHTQLAIEQNRGALEVFGPELARTLQSSSLTPAERETFMQRLAAA
                     RTAIEGYGAWLESLEKQLASGAAPARSFRLGRELYAPKFEYDIQSGGTAEALYERAVA
                     ERARLIERMSLLSDLLWPKYFPNDPVPADELVKIGQVIAKLSEQHVPREQLVATVKEQ
                     LPELERWVREHDLLELDASKPLMVRETPLHKRGIAGASIDAPGPYDPDAPTYYNVTPL
                     DDLSADRAESWLREYNQWMLPVLNIHEAVPGHYVQLVYANRSPSRIKSIFGNGAMIEG
                     WAVYAERLMLESGYGDDKAESWLIYSKWNLRSVTNTILDYGVHVRGMTEDEAMDLLTR
                     QAFQTEQEAREKWRRVTLTSVQLTSYFSGYSAIVDLREQLKREHAGSFDLKRFHEQFL
                     SYGNAPVAVIRELMWPRASSR"
     gene            complement(2525..4519)
                     /locus_tag="VM692_10955"
     CDS             complement(2525..4519)
                     /locus_tag="VM692_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ52730.1"
                     /translation="MPLSDHEQSILDGVAAGLIVVERNRAIARWNSWMVSASRRAAAD
                     VTGKTLAEVFPDAKIDRILRAIESALAGGASTLLTNALHPELLPLQTRTGRPLLHDVV
                     VAPLPGDVPAGCLVHVIDVTESTRRERYLRDRHNARYYALVESAPDVILNIDGEGIIR
                     LANAAASRHFGYSADELVGQTAARLFEPESAWLDIGHAFRSGSASTSPVEVRVRLKDG
                     RLRYFELSAAAWKDGSRTFTTAILRDVEDRRAAEAALRDSERQSRANERALAELNEAL
                     QESTRQLRAMDRRKNEFLATLAHELRNPLAPIANGLQLLKITKDDPVALERTRHMMDL
                     QLRQMVRLIDDLLDVGRISNDRLTLNKELTTLDRVIRQAIETSSPLIYAQQHTLSIDL
                     PPHELALEADAVRLTQVFANLLNNAAKYTPARGNIVIKGEERDGLAIVRVIDNGIGIP
                     RDFLPQVFEMFVQVDKSLERTQGGLGIGLSLVKRLVEMHGGTVEAVSRGPNLGSEFIV
                     RLPLTKARAPAADATAEPKPAPGHNRRRILVVDDNKDAAVTLALLLGMLGHDTRTAYD
                     GLEAIEVAREFRPEVVLLDIGMPRLNGYDTARRLRGEPWGREMLLVALSGWGQDADRL
                     KSSSAGFDIHLVKPVDVAEIQRLLARGHMSSRITATGALP"
     gene            complement(4524..>4732)
                     /locus_tag="VM692_10960"
     CDS             complement(4524..>4732)
                     /locus_tag="VM692_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HUQ52731.1"
                     /translation="VLRGESTRFFTLPPPPEAGGTVLFIYINFALQKRDVNGYIAMMM
                     DMPSLAALQQLLRNLIRRTAGTSI"
BASE COUNT          775 a         1584 c         1583 g          790 t
ORIGIN      
        1 tccacgcgct gctcgcgaga accgtcgtaa gcggcggcga tcgagtggtg cgcctcgacc
       61 gccgaagccg ccgcgagcgc cgcgacgatc gcaaggccgg cccgctgcat ccgccgctcg
      121 cggtctcgtg tgctcatcgg cctatcctag ctcgcggcgc aggcctcggc acgtgatgga
      181 cgccggccga agcgcggcgt aggttagctc caaggacact cgtgaggggg gagcaccgtg
      241 ttcgcgctca atcgaattct cgtcccgacg aatctcggcg acccgtcgcg cgcagcgatc
      301 aactacggcg tcgcgttcgc gcgccaattc ggcgcgaagc tgttcctgct gcacgtgctc
      361 gaggccaagc aatacgacgc ggtgctcgag accgagcgag tgctcgaggt cctgtcgccc
      421 gacgaggccg agcgccgggc gagcgagccg acgccgcagg aaatcgcacg taacgcggcg
      481 cggcatgatc tcggccagct gttgtcgcag gaggacgagc tcgcgacggg cgcggagtat
      541 ctgctgcgcg cgtccggcgt cgcgggcgcc ggcgaagcca tcgtcgcctg cgcacgcgag
      601 cttgacgtcg agctgatcgt catgggcaag caccggctcg gcttcgtcga gcacctgctc
      661 gccggcagcg tcgccgagaa ggtcatgcgc cacgcgccgt gccccgtgct gatcgtgcag
      721 caccccgagg gcgcgggtgc gcaagcgcac gagcgccagt tcgtcgtcgc cgacgcgccc
      781 cccgtcgggg acttccaagg gtcgccggcg gcgcgcgcca tgccatgatg tggcgagccc
      841 tgttgcttgc gggtatcgca accatgacgc ttggctcccc ggccgccgtg aaggcagccg
      901 acgaagattt tcaacgtgcg ttcggcgacg atttcttcga tcgctactgg gagctgtcgc
      961 cgggcgtcgc gatctcctac gggttctaca agtacgccga ccggctcatc gtgcccgacg
     1021 atgccgcgcg caagacgtcg ctcgcgcagc tcgaccgctg gctggcgcag ctcgcgcgca
     1081 tcgaccccgc cgatctgagc cccggtgtcc gggcggatcg cgtcatgctc gagaaccaat
     1141 tgcgcggcga acgttgggcg ctgaccgact tgcgcgcctg gcagtgggac ccgtcgatct
     1201 acaacgtcgc ggagccgttc gcgctgctgc tgagcttgga ctacgcgccg ctcgacgagc
     1261 gcttgcgcgc gttctcgaag cgcctaacga acgtgccggc atactacgct gcggccaagg
     1321 cgagcgtcgc cgggccaacg cgcgagcaca cgcagctcgc gatcgagcag aaccgcggcg
     1381 ccctcgaggt attcggcccg gagctggcgc gtacgctgca gagctcgagc ctgactccgg
     1441 ccgagcgcga gacgttcatg cagcgcctgg ccgcggcgcg tacggcgatc gagggctacg
     1501 gtgcctggct cgagagcctc gagaagcagc tcgcgagcgg cgccgcgccg gcccgctcgt
     1561 tccgcctcgg ccgcgagctg tacgcgccga agttcgagta cgacatccag tcgggcggca
     1621 cggccgaggc gctctacgag cgcgccgtcg cggagcgcgc ccggctcatc gagcgcatgt
     1681 cgctgctgtc ggacttgctg tggccgaagt acttcccgaa cgaccccgtc cctgccgacg
     1741 agctcgtgaa gatcggccag gttatcgcga agctgtcgga gcagcacgtg ccgcgcgagc
     1801 agctcgtggc gacggtgaaa gagcagctcc ccgagctcga gcgctgggtt cgcgagcacg
     1861 atctgctcga gctcgacgcg tcgaagccgt tgatggtccg cgaaacgccg ctgcacaagc
     1921 gcggcatcgc aggcgccagc atcgatgcgc cggggccgta cgaccccgat gcgccgacct
     1981 actacaacgt gacgccgctc gacgatctat cggccgaccg cgccgagagc tggctgcgcg
     2041 agtacaacca gtggatgctg cccgtgctga acattcacga ggccgttccc gggcattacg
     2101 tccagctcgt ctacgccaac cgcagcccga gccgcatcaa atcgatcttc ggcaacggcg
     2161 cgatgatcga gggctgggcc gtctacgccg agcgcttgat gctcgagtcg ggctacggcg
     2221 acgacaaggc ggagtcttgg ctcatctatt cgaagtggaa cctgcgcagt gtcacgaata
     2281 cgatcttgga ctacggcgtg cacgtgcgcg gcatgaccga ggacgaggcc atggatctct
     2341 tgacgcgcca agcgtttcag acggagcaag aagcgcgcga gaagtggcgc cgagtcacgc
     2401 tgacgagcgt gcagctcacg agctatttct cgggctattc ggcgatcgtc gacttacgcg
     2461 agcagctgaa gcgcgagcac gccggcagct tcgatttgaa gcgcttccac gagcaattct
     2521 tgagctacgg caatgcgccc gtcgccgtga tacgcgagct catgtggccg cgcgcgagca
     2581 gccgctgaat ctccgcgaca tcgactggct tgacgagatg gatgtcgaag ccggcgctgc
     2641 tcgatttcaa tcgatcggca tcttggcccc agccgctcag cgctacgagc agcatctccc
     2701 tgccccaggg ctcgcctcgt agtcggcgcg ccgtgtcgta accgttgagc ctcggcatgc
     2761 cgatgtcgag cagaacgacc tcgggacgaa attctctcgc gacctcgatg gcctcgagac
     2821 cgtcgtaggc cgtgcgcgtg tcgtgcccca gcattccgag caacagcgcc aacgtgacgg
     2881 ccgcgtcctt gttgtcatcg acgacgagga tgcggcgccg gttgtgcccc ggcgccggtt
     2941 tcggctcggc cgtggcgtcg gcggccgggg cgcgcgcctt cgtcagcggc aaccgcacga
     3001 tgaactcgct gccgagattc gggccgcggc tgacggcttc gacggtgccg ccgtgcatct
     3061 cgacgaggcg cttgacgagc gacagcccga tacccaaccc gccttgcgtg cgctcgagcg
     3121 acttgtcgac ttgcacgaac atctcgaaaa cctgcggtag gaaatcccgt gggatcccga
     3181 tgccgttgtc gatgactcgg acgatggcca agccgtcgcg ctcttcgcct ttgatcacaa
     3241 tgttgccgcg cgccggcgtg tacttcgcgg cgttgttcaa cagattggca aacacctgag
     3301 tgagccgaac cgcatcggcc tcgagcgcga gctcgtgcgg cggaagatcg atcgacagcg
     3361 tgtgctgttg ggcgtagatc agcgggctac tcgtctcgat agcttggcgg atcacgcggt
     3421 cgagcgtcgt caactccttg ttcagcgtta gccgatcgtt gctgatgcgg ccgacgtcga
     3481 gcaggtcgtc gatgagccgg accatctggc gcaactgcag atccatcatg tgtcgcgttc
     3541 gctcgagcgc taccggatcg tccttcgtga tcttcagcag ctgcaaaccg ttggcgatcg
     3601 gcgccaaggg attgcggagc tcgtgcgcca gcgtcgcgag aaactcgttc ttgcgcctat
     3661 ccatcgcgcg cagctgcctc gtgctttctt gcaacgcctc gttgagctcg gcgagggccc
     3721 tctcgttggc ccgcgattgg cgctcgctgt cgcgcagcgc ggcctccgcc gcgcgcctgt
     3781 cctcgacgtc gcgcagaatg gccgtggtga acgtgcggct gccgtccttc caggccgcgg
     3841 ccgaaagctc gaaatagcgc agccggccgt ccttgaggcg aacgcggacc tcgacggggc
     3901 tcgtcgacgc cgagccgctg cggaaggcgt gccctatgtc gagccaggca ctttcgggct
     3961 cgaacaagcg agcggccgtc tggccgacga gctcgtcggc cgaatagccg aaatggcgcg
     4021 acgcggccgc gttcgcgagc cggatgatgc cttcgccgtc gatgttgaga atcacgtcgg
     4081 gcgcgctttc gacgagcgcg tagtagcgcg cgttgtgacg atcgcgaaga taacgctcgc
     4141 ggcgcgtcga ctccgtgacg tcgatgacgt gcacgaggca gccggccggc acgtcgcccg
     4201 ggagcggcgc aacgacgacg tcgtgcagca gcggccgtcc cgtgcgcgtc tgcagcggca
     4261 acagctcggg atgcagcgcg ttcgtcagca gcgtcgaagc accgcccgcc aacgctgatt
     4321 cgatggcacg cagaatcctg tcgatcttcg cgtcgggaaa aacttcggca agcgtcttgc
     4381 ccgtgacgtc ggctgccgca cgccggctcg ccgacaccat ccacgagttc caccgggcga
     4441 tggccctgtt tcgctcgaca acgatcagcc cggcggcgac gccgtccaag atcgactgtt
     4501 cgtgatcgct cagcggcatc gttctagatc gatgtgccgg ccgtgcggcg gatcaggttg
     4561 cgcaacaact gctgcaaggc cgcgagcgaa ggcatgtcca tcatcatggc gatgtagccg
     4621 ttgacgtcgc gcttttgcaa cgcaaaattg atgtagatga acagcaccgt gccgccggcc
     4681 tccggcggcg ggggcagggt gaaaaaccgc gtgctttcgc cgcgcagcac ct
//
LOCUS       DATLEE010000508         2650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5793875, whole genome shotgun sequence.
ACCESSION   DATLEE010000508 DATLEE010000000
VERSION     DATLEE010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5793875"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VM692_10965"
     CDS             complement(<1..697)
                     /locus_tag="VM692_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ52732.1"
                     /translation="MPETVLRVRDLRKAFQDVVAVNGLDLEVQAGECFGLLGPNGAGK
                     TTTIEICEGLTARDSGEVQVLGLKWEADASELKQRLGIQLQETQLSEKLTVDETIRLF
                     RSFFHKGPTPEHVIGLVQLEEKRRARVGTLSGGQKQRLALACALVGDPDLLFLDEPTT
                     GLDPQARRQLWELIEEFKRAGRTILLTTHYMDEAERLCERVAIMDHGKIIATGSPREL
                     VASIGVEHVVEFSV"
     gene            859..>2650
                     /locus_tag="VM692_10970"
     CDS             859..>2650
                     /locus_tag="VM692_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUQ52733.1"
                     /translation="MKAWRLAIRNFARGVRAGRLTVLMIALTVAVAAITSIGFFIDRV
                     RASVAQEAAGLLAADLRETGSDPLKPADLAEAQSRGLRTAEVTSFPTVVVAGEMSTLA
                     SLYAVSDDYPLRGQVRLADAVGAASYRVSGAPPAGSVWLEDGLLARLQADVGDRVSIG
                     AREFLVERVLEHRPDQSLGFDTFAPSLMMNLGDLASTELIRPGARATYAQLFAGDADD
                     VAAFKQWFESGPERRLQTAGRSEERVDRAINRAEQFLSLASMIALLLAATAVAVSARR
                     YAAQQVEGVALLKCLGASQALVLAATLLELAVIGVATGAAGGALGYVAQLGLGTLVED
                     LMELELPAPSLAPGVSGVFVAMAVLAGFAMPAMLSLRTVPPLRVLRQDAVPKTVPTFV
                     SYGAAAGAVLGLLAWLVGTSEMLVVSVVGLGAGAVLLYIAAHALVRLIGPLRSGAGVA
                     WRYGLANIARRRADSVAQVMAFGFGLTVLLLLSGVRTGLLDTWYASLPTDAPNNFMIN
                     IQREEADGLRSLFMEYGQEPPELVPMVRGRLTAVDGEDVMSRRMRGEGGGGWLFREAN
                     LTWAEELDATNELVAGEWWAPGTERAEISVE"
BASE COUNT          373 a          917 c          926 g          434 t
ORIGIN      
        1 caaccgaaaa ctcgacgacg tgctcgacac cgatagaggc gacgagctcg cgcgggctcc
       61 ccgtggcgat gatcttgccg tggtccatga tcgcgacgcg ctcgcagagc cgctcggctt
      121 cgtccatgta gtgcgtcgtc agcaagatcg tgcgtcccgc gcgcttgaat tcctcgatga
      181 gctcccaaag ctggcggcgc gcctgcggat cgaggcccgt cgtcggctcg tcgaggaaca
      241 gcaggtcggg atcgccgacg agcgcgcacg ccagcgcgag ccgctgcttc tgaccgcccg
      301 acagcgtgcc gacgcgcgcg cgccgcttct cctcgagctg cacgagcccg atgacgtgct
      361 cgggcgtcgg tcctttgtga aaaaagctgc ggaacagccg gatggtctcg tcgaccgtga
      421 gcttctccga cagctgcgtc tcttgcagct ggatgccgag ccgctgcttc agctcactcg
      481 cgtcggcctc ccacttgagg ccgagcacct gaacttcgcc ggagtctcgc gcggtgagcc
      541 cctcgcaaat ctcgatcgtc gtcgtcttgc cggcgccgtt cgggccgagc agcccgaagc
      601 actcgccggc ctgcacctcg aggtcgaggc cgttgacggc gacgacgtct tggaacgcct
      661 tgcgaagatc gcggacgcgg aggaccgtct cgggcatgga cgtgaaggtt tccgtggcgg
      721 caattgggat cggctgcgca ttatcgcgtc gattgagcgc gccgtcagct caattgtggt
      781 caggctctaa gaacgagcgc tgtgcccgaa acgttcgcgt agactggctt tcgtcgcgac
      841 acggacatca gccgaacgat gaaagcctgg cgcctcgcca tccgcaattt cgcccgcggc
      901 gtccgcgcgg gacggttgac ggtactcatg attgcgctga cggtcgcggt cgcggccatc
      961 acgagcatcg gcttcttcat cgaccgcgtg cgcgcgagcg ttgcgcagga agcggccggc
     1021 ttgctcgccg cggatctgcg cgagaccggc agcgacccgc tcaaacccgc cgatctcgcc
     1081 gaagcgcaaa gccgcgggct gcgcacggcg gaggtcacgt cgttcccgac ggtcgtcgtc
     1141 gccggcgaga tgagcacgct cgcgagcctg tacgccgtca gcgacgacta tccgctgcgc
     1201 ggccaagtgc ggctcgccga tgccgtcggc gccgcgagct atcgcgtcag cggcgcgccg
     1261 ccggccggca gcgtctggct cgaggacgga ctgctcgcgc gcctgcaagc cgacgtcggc
     1321 gatcgcgtct cgatcggcgc gcgcgagttt ctcgtcgagc gcgtgctcga gcatcggccc
     1381 gatcagtcgc tcggcttcga cacgttcgcg ccctccttga tgatgaatct cggcgacctc
     1441 gcgtcgaccg agctcataag acccggcgcc cgcgccacct acgcgcagct gttcgccggc
     1501 gacgccgacg acgtcgcggc cttcaaacaa tggttcgagt cgggccccga gcggcgcctg
     1561 cagacggcgg gccgctccga ggagcgcgtc gaccgcgcca tcaatcgcgc cgagcagttc
     1621 ctgagcttgg ccagcatgat cgcgctgctg ctcgcggcga cggccgtcgc ggtctcggca
     1681 agacgctacg cggcgcagca agtcgagggc gtcgcgctcc tgaaatgtct cggcgcgtcc
     1741 caggcgctcg tgctcgccgc gacgttgctc gagctcgccg tcatcggcgt cgcaaccggc
     1801 gccgcgggcg gcgcgctcgg ctacgtcgcg cagctcggcc tcggcacgct cgtcgaggac
     1861 ctcatggagc tcgagctgcc ggcgccgagc ttggcgcccg gcgtcagcgg cgtcttcgtc
     1921 gcgatggcgg tgctcgcggg gttcgcgatg ccggcgatgc tgagcttgcg cacggtgcca
     1981 ccgctgcgcg tgctgcggca ggacgcggtg ccgaagaccg tgccgacgtt cgtgtcgtac
     2041 ggcgccgcgg caggcgccgt gctcggcctg ctcgcgtggc tcgtcggcac gagcgagatg
     2101 ctcgtcgtgt ccgtcgtcgg attgggtgcc ggtgccgtac tcctttatat agcggcacac
     2161 gcgctcgtgc ggctgatcgg gccgctgcgc agcggcgcag gcgtcgcctg gcgctacggg
     2221 ctcgcgaaca tcgcgcgccg tcgcgccgac agcgtcgcgc aggtcatggc gttcggcttc
     2281 gggctcacgg tgctgctgct cctgtcgggc gtgcggaccg gcctgctcga cacgtggtac
     2341 gcgagcctgc cgaccgacgc gccgaataac ttcatgatca acatccagcg cgaggaagcc
     2401 gacggcttgc gctccttatt tatggagtac ggccaagagc cgcccgagct cgtgccgatg
     2461 gtgcgcggcc ggctcacggc cgtcgacggc gaggacgtca tgagccgccg catgcgcggc
     2521 gaaggcggcg gcggttggct gttccgcgag gcgaacctga cgtgggccga ggagctcgac
     2581 gcgacgaacg agctcgttgc cggcgagtgg tgggcgcccg gcaccgagcg cgccgagatc
     2641 tcggtcgaac
//
LOCUS       DATLEE010000509         4495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8489715, whole genome shotgun sequence.
ACCESSION   DATLEE010000509 DATLEE010000000
VERSION     DATLEE010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8489715"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VM692_10975"
     CDS             complement(<1..1065)
                     /locus_tag="VM692_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUQ52734.1"
                     /translation="MNTRAMKRVAHALVAAALTCATAQAQLLGDPPRGEATERHLPGE
                     IKPTQWAHDPNLLSTENGDRFEMREVAAQELETIKLTNLVPPIHFAVGVAEIPDSTVA
                     ELRKILGGMQERRNVRLHLVGHADAQPLSPALAAVFGDNEGLSRERAGEVAELLQRTL
                     GLPAEGISYEWAGDTKPVASNASEAGRAQNRRVEVEVWYDEVKQATALDEVLVAEQFR
                     QVKVCRMETVCRLRYVDGNERRTRVQNVVAPLRFGEGAVEVAPAYVEQIRQAVANLSD
                     RHNVLVKFVGYTDDAPLTERNERIYGNHVGLARAQARRVALAVQQELKLATSAVDSDG
                     RGSARPLGSNATAQGRALNRR"
     gene            complement(1062..>4495)
                     /locus_tag="VM692_10980"
     CDS             complement(1062..>4495)
                     /locus_tag="VM692_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52735.1"
                     /translation="MSFLNNGSPVSEFITREGGTINSSGLVGQFTLPNVPPGTPVLTL
                     DKSGPATMNPAEPGDFALSVQNIGVADAFNVTLLDRLPDLPTGGMCDTTPQILSARVF
                     AADGVTPIAGKGPLTQGTDYSLAYNGALCELTLTTLSAASVIGVGGQRLIVTYRTQLD
                     SPFTYGGALTNVAGATQWYNGPSNDPMRVGYTRVLTNGTVGTVDHEDAHTVTVVPRLY
                     ADKAAVLQVDAMSPGIVDPGDVLRYTIRVYNNGSVPVTQSMLRDQVPANTTYVADTMT
                     LNGQPVGRPDGGVSPLVAGIAVNSPAAAAGTVAAGEVGIVTFDLRVNDGVPPGTVISN
                     QAVVDTSELPDLLTDGDGNPATGPEPTVVVVGNLQEVRITKSVSVVGGGPALAGATLE
                     YVVQATNVGTLPVIDVVVRDDIAVPQPGYLTFAAGSWSLNNSVNGVTVAGTLLTADYS
                     TTYGPLPPGRTVTVRFRAVLDPSLAIGTRVTNTGTVYWNNPVQTASASVSIDVGGTPG
                     VGLVNGRVWHDADFDRVYDTNEIALAGWDVELLLNGSVAHVARTSADGIYRLSSVEPN
                     YNTADTYELRFRRPGAGARTALLGRADSEFTDDLQRITDIVVPSGANLQNLNLPIDPN
                     GIVYDSISRAPIAGATVSLVTPVGGTPLPSACFYDANQQGQVTLADGYYKFDLNFADP
                     ACPSGSAYLLSVAPPSSTRYLAEVSAIIPPLSSATTAAFSVPSCPASIDDAVPATTQH
                     CEATSSEIQPPPSATPGTTGTRYYLHVTFDDSAVPGSSQIYNNHIPLDLNLDESINIT
                     KTTPLVHVSRGQLVPYIITVTNGIDVTLPDVTIVDRFPAGFRYVEGSARLDGIPREPT
                     MTGRELSWSGLTVTPDNRHELKLLLAVGSGVGEGEFVNRAQAMSGITGGPASTEAQAR
                     VRIIPDPALDCTDVIGKVFDDENRNSLQDDGERGIPGVRLATARGLLATTDQFGRYHI
                     TCAITPHEGRGSNFVVKLDDRTLPSGYRGSTESLQVQRATRGKMLEFNFGASIHRVIG
                     LDLADAVFEPGSIEIRELWMPRLDLLLEELRAAPAVLRLSYLADLEDPQLVDARVKTL
                     RRYIENAWLEGAGDAGPSYELTIEPEVFWRRDEPTDARAHLGSPRRGERRRGNRE"
BASE COUNT          652 a         1504 c         1597 g          742 t
ORIGIN      
        1 acggcgattc agcgctctgc cctgcgccgt ggcgttcgag cccagcgggc gcgccgagcc
       61 gcggccgtcg ctgtcgacgg ccgacgtcgc gagcttcagc tcctgctgga cggccagcgc
      121 gacgcgacgc gcctgcgcgc gcgcgaggcc gacgtggttg ccgtagatgc gctcgttgcg
      181 ctcggtcaac ggcgcgtcgt cggtgtagcc gacgaacttg acgagcacgt tgtggcggtc
      241 gctcaagttc gcgacggctt ggcggatctg ctcgacgtag gccggcgcga cctcgacggc
      301 accctcgccg aagcgcaagg gggcgacgac gttctgcacg cgcgtgcgcc gttcgttgcc
      361 gtcgacgtag cgtaggcggc agacggtctc catgcggcag accttgacct ggcggaactg
      421 ctcggcgacg aggacctcgt cgagcgccgt ggcctgcttg acctcgtcgt accagacctc
      481 gacctcgacg cggcgattct gcgcgcggcc ggcttcgctc gcgttcgacg cgacgggctt
      541 cgtgtcgccg gcccactcgt acgagatacc ttcggccggc aggccgagcg tgcgctgcaa
      601 cagctcggcg acctcgccgg cgcgctcgcg cgacaaacct tcgttgtcgc cgaacacggc
      661 cgcaagcgcc ggcgacagcg gctgcgcgtc ggcgtggccg acgaggtgca gccgcacgtt
      721 gcggcgctcc tgcatgccgc ccaagatctt gcgcagctcg gcgaccgtgc tgtcgggaat
      781 ctcggcgacg ccgacggcga aatggatcgg cggcacgagg ttcgtcaact tgatcgtctc
      841 gagctcctgg gccgcgacct cgcgcatctc gaagcggtcg ccgttctcgg tgctcaagag
      901 attcgggtcg tgcgcccact gcgtcggctt gatctcgccg ggcaagtggc gctccgtggc
      961 ctcgccgcgc ggcggatcgc ccagcaattg cgcctgcgcc gtcgcgcaag tcagcgcggc
     1021 ggcgacgagc gcgtgcgcta cgcgcttcat ggctcgcgta ttcattcgcg attccccctg
     1081 cgccgctccc cccttcgggg actcccaagg tgcgcgcgtg cgtcggtcgg ctcgtcacgc
     1141 cgccagaaga cctcgggctc gatcgtgagc tcgtagctcg ggcccgcatc gccggcgcct
     1201 tccagccacg cgttctcgat gtagcggcgc agcgttttca cgcgcgcgtc gacgagctgc
     1261 gggtcctcga gatcggcgag atacgacagg cgcagcacgg ccggcgccgc gcgcaactct
     1321 tcgagcagca ggtcgagccg cggcatccag agctcgcgga tctcgatcga gcccggctcg
     1381 aacacagcgt ccgcgagatc gaggccgatc acgcgatgga tcgacgcgcc gaaattgaac
     1441 tcgagcatct tgccgcgcgt cgcgcgctgc acctgcaacg actcggtcga gccgcggtag
     1501 ccgctcggca gcgtccggtc gtcaagcttc acgacgaagt tgctgccgcg gccttcatgc
     1561 ggcgtgatcg cgcacgtgat gtgatagcgg ccgaattgat ccgtcgtcgc gagcaggccg
     1621 cgcgccgtcg cgagccgcac gccgggaatg ccgcgctcgc cgtcgtcctg caggctgttg
     1681 cggttctcgt cgtcgaatac cttgccgatg acgtccgtgc agtcgagcgc ggggtccgga
     1741 atgatgcgca cgcgcgcttg cgcctcggtc gacgcggggc cgcccgtgat gccgctcatg
     1801 gcctgcgcgc ggttgacgaa ctcgccttcg cccacgccag agccgacggc gagcagcagc
     1861 ttcagctcgt ggcgattgtc gggcgtgacg gtcaagccgc tccacgagag ctcgcggccc
     1921 gtcatcgtcg gctcgcgtgg aatcccgtcg agccgcgccg agccttcgac gtagcggaag
     1981 ccggccggga agcggtccac gatcgtgacg tccggcagcg tcacgtcgat gccgttcgtg
     2041 accgtaatga tgtacggcac gagctggccg cgcgacacgt gcacgagcgg cgtcgtcttc
     2101 gtgatgttga tcgactcgtc gaggttgagg tcgagcggaa tgtggttgtt gtagatctgg
     2161 ctcgagcctg ggaccgcgct gtcgtcgaac gtcacgtgca agtagtaacg agtgccggtc
     2221 gtgcccggcg ttgccgaggg aggcggctga atttccgacg acgtcgcctc gcagtgctgc
     2281 gtggttgcgg gcaccgcgtc gtcgatggac gccgggcacg acggcacgga gaacgcggcc
     2341 gtcgtcgccg acgacagcgg cggaatgatc gccgacacct cagccaggta gcgcgtcgac
     2401 gagggcggcg cgacgctgag cagatacgcg ctgccgctcg ggcacgccgg gtccgcgaag
     2461 ttcagatcga acttgtagta gccgtcggcg agcgtgactt ggccctgctg gttcgcgtcg
     2521 tagaagcacg ccgacggtag cggcgtgccg ccgaccgggg tcacgaggct gaccgtagcg
     2581 cccgcgatcg gcgcccggct gatcgagtcg tagacgatgc cgttcgggtc gatcggcagg
     2641 ttcaggttct gcaggttggc gcccgacggc acgacgatgt cggtgatgcg ctgcaagtcg
     2701 tcggtgaact ccgagtccgc gcggccgagt agcgccgtgc gcgcgccggc gccgggcctg
     2761 cggaaccgca gctcgtaggt gtccgccgtg ttgtagttcg gctcgacgct gctcaagcgg
     2821 tagatgccgt cggccgacgt gcgcgcgacg tgcgcaaccg aaccgttcaa caagagctcg
     2881 acgtcccagc ccgcgagcgc gatctcgttc gtgtcgtaga cgcggtcgaa gtcggcgtcg
     2941 tgccagacgc gcccgttgac gagcccgacg cccggcgtgc cgccgacgtc gatcgagacg
     3001 ctggcgcttg ccgtctgcac ggggttgttc caatagacgg tgccggtgtt cgtgacgcgc
     3061 gtgccgatgg cgagactcgg atcgagcacg gcgcggaagc gcacggtgac cgtgcggccc
     3121 ggcggcagag gaccatacgt cgtcgagtag tcggcggtca gcaaagtgcc ggcgacggtg
     3181 acgccgttca ccgagttgtt caacgaccac gaccctgcgg cgaacgtcag gtagcccggc
     3241 tgcggcaccg cgatgtcatc gcggacgacg acgtcgatga cgggcagcgt gccgacgttc
     3301 gtggcctgca cgacgtactc gagcgtcgcg cccgcgagcg cgggcccgcc gccgacgacg
     3361 ctgacgctct tcgtgatgcg cacttcctgc aagttgccga cgacgacgac ggtcggctcg
     3421 ggtcccgtcg ccgggttacc gtcaccgtcg gtcaagagat ccggcagctc ggaggtatcg
     3481 acgacggcct ggttcgagat cacggtgcca ggcggcacgc cgtcgttgac gcgcaaatcg
     3541 aaggtaacga ttccgacctc gcctgcggcc accgtgccgg cagcggcggc cggcgaatta
     3601 acagcgattc ccgcgacgag cggcgacacg ccgccgtcgg gccggccgac gggttggccg
     3661 ttcaatgtca tcgtgtcggc gacgtaggtc gtgttcgccg gcacttggtc gcgcagcatc
     3721 gattgcgtga ccggcacgct gccgttgttg tagaccctga tcgtgtagcg cagcacgtcg
     3781 ccgggatcga cgatgccggg cgacatcgca tcgacttgca gcaccgcggc tttgtcggcg
     3841 tagaggcgcg gcacgacggt cacggtgtgc gcgtcctcgt ggtcgacggt gccgaccgtg
     3901 ccgttcgtca gcacgcgcgt gtagccgacg cgcatcgggt cgttgcttgg gccgttgtac
     3961 cactgcgtcg cgccggcgac gttcgtcaac gcgccgccgt acgtgaacgg cgaatcgagc
     4021 tgcgtgcgat acgtgacgat caggcgttgg ccgccgacgc cgatcacgga agcggcgctc
     4081 aacgtcgtca acgtgagctc gcagagggcg ccgttgtagg ccagcgagta gtcggtccct
     4141 tgggtcaggg gtcctttgcc cgcgatcggg gtcacgccgt ccgcggcgaa aacccgcgcg
     4201 ctgaggatct gcggcgtcgt gtcgcacatg ccgcccgtcg gcaggtccgg cagccgatcg
     4261 agcagcgtga cgttgaacgc gtcggcgacg ccgatgttct gcacgctcaa ggcgaaatcg
     4321 ccgggctccg cgggattcat cgtcgccggg cccgacttgt ccagggtcaa caccggcgtg
     4381 ccgggcggca cgttcggcaa cgtgaactgg ccgacgaggc ccgagctgtt gatcgtgccg
     4441 ccttcccgcg tgatgaactc cgacacgggg ctgccgttgt tcaggaagct catcg
//
LOCUS       DATLEE010000510         7381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4289209, whole genome shotgun sequence.
ACCESSION   DATLEE010000510 DATLEE010000000
VERSION     DATLEE010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4289209"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1812)
                     /locus_tag="VM692_10985"
     CDS             complement(163..1812)
                     /locus_tag="VM692_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ52736.1"
                     /translation="MRTIVAAAAVLCAMRAFAQPFPPGYVDPAPLLAAAAREIGEGEL
                     RCITFSGTGYAGAVGQTFENAVNIDWPRIDSLANYTRTINWETGTGVETFDRKPGVSP
                     ASWKYGLGWQGGTPTQMATRQTHIVNDRYAWHIDGDGEPVAVPPELAELYQLDLWLNP
                     AGFIKAARLPGANPVAFWRWEQLEKGRDGNVVAPEKVKVVAITMLGKYRVDATINSRN
                     VITRIKTTVGEPALGDFNIEHESTNFVAVGNVKWPTAWHSHHGWDDNWQFYRQSTGHN
                     GYGGQFANVRPNDCGATVPVPPSVAAATFPVTVTVDELADGVYRLGGGPANSYLVEFD
                     DFVAVFEAPGNEQRSLAVIDTIAKLAPDKPIRFVVSSHPHFDHIGGLRTYLHIGATVV
                     AHAKNIPFLNSDVLSYEPRTVAPDIVSRWPPTEVSEGYNYEAVQENYVITDNRRILRI
                     YYVQPLQHVEGMLMAYLPAERIAFEADLFDTHEPHTGPPKPAMTSFWNQVRRMKLDVG
                     TIAPVHGRPVPWSEFQNAMGGAANLCETVGAGGSVAWVPCK"
     gene            complement(1912..2331)
                     /locus_tag="VM692_10990"
     CDS             complement(1912..2331)
                     /locus_tag="VM692_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52737.1"
                     /translation="MKPHAIAALASALLFCAATPALAHHSFAAEFDANQPVTLKGTIV
                     KMEWINPHSWLHIDVKNPDGTTTPWMIEGATPNTLLRRGFTREAVKAGTEITIVGYRA
                     KNGANRANGRDLVLPDGSRLFMSSSGTGAPEEPPAEQ"
     gene            complement(2328..3320)
                     /locus_tag="VM692_10995"
     CDS             complement(2328..3320)
                     /locus_tag="VM692_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52738.1"
                     /translation="MRMRTTALSAAVFVALAALSTSSQGQSAAAAAEPYTVPRTSWGH
                     PDLQGVWDYRTITPLERRPELGDREFYTEEEIAQLEGRATRRMTGPPDPNAPANLVHA
                     EYLTDPGRMLDESHRTSLIVDPPNGRMPPLTPEAQERQAQGRPPAGAPARVGGRADSY
                     TDRSMLERCITRGAAGAPMPTLYNNNVRITQGPNSVALVHEMVHETRIVPLDDTPFGN
                     VRTYQGESRGHWEGDTLVVETRNFNGKAPFRGAGTNLKIVERYTRTAPDKLDFKVTFE
                     DDTQWTQPWTAAFTMNTGEGELYEYACHEGNYGLRNILENARDEERKAAAEKRR"
     gene            3493..5808
                     /locus_tag="VM692_11000"
     CDS             3493..5808
                     /locus_tag="VM692_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ52739.1"
                     /translation="MTSTAARVGVRSVARGALQAGAWLGAATLASGVHQAVAQPEHGE
                     SNLTGDVVEVFGERLEQGLGTRYTAPLLETPQTITLVPRELIDQQNLLTMREILSTLP
                     GITFTAGEGGGGYGDGVNLRGYTATNDISTDGVRDSAQYSRTDPFNLEQLELVSGANS
                     VYTGAGSVGGSINLVTKTPRGMAGTVLEVAGGGDSYGRLTLDSESQVGDVADVRLNVM
                     SHRNDVAGRDVERFERWGIAPSVELGGDSNTRVSVALLHQEDDNIPQYGVPYALNAFN
                     DGPLRGVPTSAFYGYSNLDKQEIEVDAFTVIMDHEFADNLSLRNLTRWQQVDQLTFAN
                     QPQGAWCVETGVNPWTGAACAAPGTFVPSRSGTVRDTTNEIVVNQTDFTFDFATGKVE
                     HTLVAGLSFSHETYRRANGNGLRNPLGATPNPVLPAMSIANPDHVYTGPVNFIVAQRA
                     DGEVDNEAAYVFDRIQLTERLEVNAGLRFEHNDAFSTLGNVAVPYPAPPAQPLVTETA
                     PADNVDDLGSYRVGLVYKPGDNSSIYIAHGNSETPSQASVNGTCDILTTCSVAPEEAQ
                     TLEIGGKVDLNGQLSLTAAVFRNERSNFRVPSGDPTIPEQQLDGNSRVDGVALGAAGA
                     IGTKWSVFANYTYLDSEVVQSVSDRSLLGGTIDIVAGDPLPNTPERSASVWATYRISD
                     AFTVGYGLTYQGEYTFARASATADLYYTPDYVVHRAMATYEINDNLGLQLNVDNVTDE
                     VYYERIRNNATNGWATPGAARSGVLSVTWKL"
     gene            5840..6523
                     /locus_tag="VM692_11005"
     CDS             5840..6523
                     /locus_tag="VM692_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HUQ52740.1"
                     /translation="MMVQIPAVLTAAQVAEVRRLVDSADWVDGNATSGAQAALTKRNR
                     QLPEDSPPGKRAGEIVLDALGRNSLFMAAALPAKVWPPLFNRYGGGEQFGVHVDNAVR
                     LKRGGVERLRSDLSATLFLSDPETYEGGELTVEDTYGAHSVKLAAGDLILYPASSLHH
                     VTPITAGERLASFFWVQSMIRDDAQRRVLFDLDVAVQRLAVELGQAHASVVALTGTYH
                     NLLRMWAEV"
     gene            6604..>7381
                     /locus_tag="VM692_11010"
     CDS             6604..>7381
                     /locus_tag="VM692_11010"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52741.1"
                     /translation="MSKTTQIATLGALVLAAPALAHHGFGRFDPTKMVMLEGTVTGID
                     FVNPHAYLYFDAKAADGNVTKMRCEMRAATVLRRSGWSPEMFTPGKHVAVEGNPHRDD
                     PASCYIETLTLGDEKLERYQQLTSATQNTNRPARAASGEPNIYGDWAQEQYLIARPPE
                     GRGGLVPKSMVAGIESGQIKIADVPGAGWGASTVTFTPAGQAAADALRARPPDQNERA
                     RCEITSVLFDWVFDGPVNRITKGADSVTMEYGRGLKRTIYT"
BASE COUNT         1206 a         2389 c         2489 g         1297 t
ORIGIN      
        1 tggaaggagc cgccatcaca cgtcggctcc tccccaggtc ctcgagcacg cctccgcgct
       61 gcgcccggcg aaccgcgtgt caacgtccga tgcgtgcgac cagcgcccag agagtgattg
      121 ggatccattg acgtggaacg gctgccgaac acatgtgtcg cgctacttac aaggtaccca
      181 agccactgaa ccgccggcgc ctactgtttc gcagagattg gccgcgccgc ccatcgcatt
      241 ctgaaactcg gaccacggca cgggacgacc gtgcaccggt gcgatggtcc ccacgtctag
      301 cttcatacgc ctgacctgat tccagaagct ggtcatcgcc ggcttcggcg gtccggtgtg
      361 cggttcatgg gtgtcgaaca ggtctgcttc gaaggcgatg cgctcggcgg gcagatacgc
      421 catcaacatg ccctcgacgt gctgcaacgg ctgcacgtag tagatgcgca ggatgcgccg
      481 gttatccgtg atgacgtagt tttcctgcac ggcttcgtag ttgtagcctt cggagacttc
      541 ggtgggcggc cagcgcgaga cgatgtcggg cgcgaccgtg cgcggctcgt agctcaacac
      601 gtcgctgttc aggaacggta tgttcttcgc gtgagcgacg acggtcgcgc cgatgtgcag
      661 ataggtgcgt aagcccccga tgtggtcgaa atgcgggtgc gagctcacga cgaaccgaat
      721 cggcttgtcg ggcgccagct tcgcaatcgt gtcgatgacg gccaggctgc gctgctcatt
      781 gccgggcgcc tcgaacacgg ctacgaagtc gtcgaactcg accaaatagc tgttcgcagg
      841 gccgccgccg agccggtaca cgccgtcggc cagctcgtcg acggtgacgg tcaccggaaa
      901 cgtcgcggct gcaaccgacg gcggcacggg cacggtcgcg ccgcagtcgt tcggccgcac
      961 gttcgcgaat tggccgccat agccgttgtg gccggtgctc tggcggtaga actgccagtt
     1021 gtcgtcccag ccgtgatgcg agtgccacgc cgtgggccac ttgacgttgc cgacggcgac
     1081 gaaattcgtc gattcgtgct cgatgttgaa gtcgccgagc gccggctcgc caaccgtcgt
     1141 cttgatgcga gtaatgacgt tgcgcgagtt gatcgtcgcg tcgacgcggt acttgccgag
     1201 catcgtgatg gcgacgacct tgactttctc cggcgccacg acgttgccgt cgcggccctt
     1261 ttcgagctgc tcccagcgcc aaaatgcaac ggggttcgct ccgggcagcc gtgccgcctt
     1321 gatgaaaccg gccggattca gccagaggtc gagctgatag agctcggcga gctcgggcgg
     1381 cacggcgacg ggctcgccat cgccgtcgat gtgccacgcg tagcggtcgt tgacgatgtg
     1441 tgtttggcgc gtggccattt gcgtcggcgt gccgccttgc catcccaatc cgtatttcca
     1501 cgacgccgga ctgacgccgg gcttgcggtc gaacgtctcg acgcccgtgc ccgtctccca
     1561 gttgatcgtg cgcgtgtagt tcgcgagcga gtcaatgcgc ggccaatcga tgttcacggc
     1621 gttctcgaac gtctggccga cggcgcctgc gtagccggtg ccggagaacg tgatgcagcg
     1681 cagctcgcct tcgccgatct cgcgcgccgc cgccgcgagc aatggggcag ggtccacgta
     1741 gccgggcgga aaaggctggg cgaaagctcg catggcgcac agcaccgcgg ccgccgcgac
     1801 gatcgttctc atcgtaggtc ccctctatct cccgcagcga gcccgtgccg accagcacgg
     1861 cgagacgatc gcggctgcga tcgctagcgg cttgcgcacg cggcggcgct gttactgctc
     1921 agcaggcggt tcttccggtg cgccggtgcc ggaagacgac atgaacagcc gcgagccgtc
     1981 gggcagcacg agatcgcggc cgttggctcg gttcgcgccg ttcttcgctc gatagccgac
     2041 gatcgtgatc tccgttccgg ccttgacggc ttcacgcgtg aacccgcggc gcagcaacgt
     2101 gttcggcgtc gcgccttcga tcatccacgg ggtcgttgtg ccgtcggggt tcttcacgtc
     2161 gatgtgcagc cacgagtgcg gattgatcca ctccatcttg acgatcgtgc ctttcaaggt
     2221 cacgggctga ttcgcgtcga actccgccgc gaacgagtga tgcgccaacg ccggcgtagc
     2281 ggcgcagaag agcagggcgc tcgcgagggc cgcgattgcg tgcggtttca tcggcgtttc
     2341 tccgcggcgg ctttgcgctc ctcgtcgcgc gcgttctcga ggatattgcg cagcccatag
     2401 ttgccttcgt ggcacgcgta ctcgtagagc tcgccttcgc ccgtgttcat cgtgaacgcg
     2461 gccgtccacg gctgcgtcca ttgcgtgtcg tcctcgaacg tgaccttgaa gtcgagcttg
     2521 tcgggcgcgg ttcgcgtgta gcgctcgacg attttcaagt tcgtgcccgc cccgcggaac
     2581 ggagccttgc cgttgaagtt gcgcgtctcg acgaccagcg tgtcgccctc ccaatggccg
     2641 cgcgactcgc cctgataggt gcgcacgttg ccgaacggag tgtcgtcgag cggcacgata
     2701 cgagtctcgt gcaccatctc gtgcacgagc gcgacgctat tcggaccctg cgtgatgcgc
     2761 acgttgttgt tgtagagcgt cggcatcggg gcgccggcgg cgccgcgcgt gatgcaacgc
     2821 tcgagcatgc tgcgatccgt gtacgagtcg gcgcggccgc cgacgcgcgc cggcgcgccg
     2881 gcaggcggac gtccctgagc ttggcgctct tgcgcttcag gcgtcagcgg cggcatacgg
     2941 ccgttcggcg ggtcgacgat caatgacgtg cgatgcgact cgtcgagcat gcggcccggg
     3001 tccgtgaggt actcggcgtg cacgagattc gccggcgcgt tcgggtccgg cggtccggtc
     3061 atgcggcgcg tggctcggcc ttcgagctgg gcgatctctt cctcggtgta gaactcgcga
     3121 tcgccgagct cggggcgccg ctcgagcggc gtgatcgtgc ggtagtccca gacgccctgc
     3181 agatcggggt gcccccacga cgtgcgcggg acggtgtacg gctcggctgc ggcagccgcg
     3241 gactggcctt gcgagctcgt cgatagtgct gcgagcgcca caaacaccgc tgccgagagc
     3301 gccgtcgttc gcattctcat cacgttcccc agatttctga gcacctgccg gatcgtagca
     3361 ccggccatgt tggtgccgta gagcgccgac atcgagaaac gtcttgttca gcgagatgcg
     3421 aatgagttgc gtttgagtca ggcgctggcg ataatgcgaa ctaatcgcat ttgcatctaa
     3481 gggagagggg cggtgacttc gacagcggct cgagtcggtg tgcgcagcgt ggcgcgcggt
     3541 gcgttgcagg ccggggcatg gttgggagcg gcgacgctgg cgagcggcgt gcatcaggcg
     3601 gtggcgcagc ccgagcatgg cgagtcgaac ctgacgggcg acgtcgtcga ggtgttcggc
     3661 gagcgattgg aacagggttt gggcacgcgc tacacggcgc cgctgctcga gactccgcag
     3721 acgatcacgc tcgtgccgcg cgagctcatc gaccaacaaa acttgctgac gatgcgcgag
     3781 atcctctcga ccttgcccgg catcacgttc acggccggcg agggcggcgg cggttacggc
     3841 gacggggtga acctgcgcgg ttacaccgcg acgaacgaca tctcgaccga cggtgtgcgc
     3901 gacagcgcgc aatacagccg caccgatccg ttcaatctcg agcagctcga gctcgtctcg
     3961 ggcgcgaact ccgtctacac gggcgcgggc tcggtcggcg gctcgatcaa tctcgtgacg
     4021 aagacaccgc gcggcatggc cggcacggtg ctcgaggtcg cgggcggcgg cgactcgtac
     4081 ggccggctca cgctcgacag cgagtcgcag gtcggggacg ttgccgacgt gcgcttgaac
     4141 gtgatgtcgc atcgcaacga cgtcgcaggc cgcgacgtcg agcgtttcga gcgctggggt
     4201 atcgcgccgt ccgtcgagct cggcggcgat tcgaacacgc gtgtctcggt cgcgttgctg
     4261 catcaagaag acgacaacat tccgcaatac ggcgtgccgt acgcgctcaa tgcattcaac
     4321 gatgggccgc tgcggggtgt gccgacgagc gccttctacg gctactcgaa cctcgacaag
     4381 caggaaatcg aagtcgacgc gttcacggtg atcatggatc acgagttcgc cgacaacttg
     4441 tcgctgcgca atctcacgcg ctggcagcaa gtcgatcaat tgactttcgc gaaccagccg
     4501 caaggtgctt ggtgcgtcga gaccggcgtc aatccgtgga cgggcgccgc ctgtgccgca
     4561 ccgggcacct tcgtgccgag ccgcagcggc accgtgcgcg acacgacgaa cgagatcgtg
     4621 gtcaatcaga cggactttac gttcgacttc gccaccggca aggtcgagca cacactcgtc
     4681 gcgggtttgt cgttctcgca cgagacttac cgacgcgcga acggcaacgg gctgcgcaat
     4741 ccgctcggcg caacgccgaa ccccgtgctg cccgccatga gcatcgcgaa ccccgatcac
     4801 gtctacacgg gccccgtgaa cttcatcgtc gctcagcgcg ccgacggcga ggtcgataac
     4861 gaggcggcct acgtcttcga tcgcatccag ctgacggagc ggctcgaggt caacgcgggc
     4921 ctgcgcttcg agcacaacga tgctttcagc acgctcggca acgtcgccgt gccgtatccg
     4981 gctccgccgg cgcagcccct cgtcacggaa accgcgcctg ccgacaacgt cgacgatctc
     5041 ggctcgtatc gcgtgggctt ggtctacaag cccggcgaca attcgagcat ttacatcgcg
     5101 cacggcaatt cggagacgcc gtcgcaagct tccgtcaacg ggacgtgcga catcttgacg
     5161 acttgtagcg tcgcgcccga ggaggcgcag acgctcgaga tcggcggcaa ggtcgacttg
     5221 aatggccagc tctcgctgac ggctgccgtg ttccgcaacg agcgcagcaa cttccgcgtg
     5281 ccgtcgggcg atccgacgat ccccgagcag cagctcgacg gcaactcgcg cgtcgacggc
     5341 gtcgccctcg gcgcggccgg cgcgatcggg acgaagtggt cggtgtttgc gaactacacc
     5401 tatctcgaca gcgaagtcgt gcagagcgtg tcggatcggt cgctgctcgg cggcacgatc
     5461 gacatcgttg ccggcgaccc gctgccgaac acgccagagc gctcagcgag cgtgtgggcg
     5521 acgtatcgga tctccgatgc gttcacggtc ggctacggcc tgacgtatca aggcgagtac
     5581 acgttcgcgc gcgcgagcgc gacggcggat ctgtactaca cgccggacta cgtcgtgcac
     5641 cgcgcgatgg cgacgtacga gatcaatgac aacctcggcc tgcagctgaa cgtggacaat
     5701 gtcacggacg aggtctacta cgagcgcatc cgcaacaacg cgaccaacgg ctgggcgacg
     5761 ccgggcgccg cgcgctccgg cgtgttgagc gtcacttgga agctctgagc cacggcgccc
     5821 gtcgcctggc gctctgcaaa tgatggttca gatccccgcc gtgctgacgg ctgcgcaagt
     5881 ggcggaggtg cggcgcctcg tcgattcggc ggactgggtc gacggtaatg cgacgagcgg
     5941 cgcgcaggcc gcgctcacga agcgcaaccg ccagttgccg gaggacagcc cgcccggtaa
     6001 gcgggcgggc gagatcgtgc tcgacgcgtt gggccgcaac tcgctgttca tggcggcggc
     6061 gttgccggcc aaggtctggc cgccgttgtt caaccgttat ggcggcggcg agcagttcgg
     6121 cgttcatgtc gacaacgccg tgcgcctgaa gcgtggcgga gtggaacgct tgcgcagcga
     6181 tctgtcggcg acgctgttcc tgtcggatcc agagacctat gaaggcggcg agttgaccgt
     6241 cgaggacacg tacggcgcgc attcggtcaa gctcgcggcc ggcgacttga ttctctatcc
     6301 cgcctcgagc ttgcaccacg tgacgccgat caccgccggc gagcggctcg cgtcgttctt
     6361 ctgggtgcaa agcatgatcc gcgacgacgc gcagcgccgc gtgctgttcg acctcgatgt
     6421 cgccgtgcag cggctcgccg tcgagctcgg gcaggcgcat gcgtcggtcg tggcgttgac
     6481 gggcacgtat cacaacctct tgcgcatgtg ggccgaagtt taatcggccg cggctgcttt
     6541 cccgtcccgg gcgctatact cgttgtaccg gccatcgccg tgaacgagag ttcgggggag
     6601 ctcatgtcca aaacgacgca gattgctacg ctcggcgcgc tcgtgctcgc cgcgcccgcg
     6661 ctggcgcatc acggtttcgg gcggttcgat ccgaccaaaa tggtcatgct cgaaggaacg
     6721 gtgaccggca tcgacttcgt gaacccccac gcttacctgt atttcgacgc gaaggccgcg
     6781 gacggcaacg tcacgaagat gcgctgcgag atgcgcgccg cgacggtgtt gcgccgctcg
     6841 ggctggtcgc ccgagatgtt cacgcccggc aagcacgtcg ccgtcgaggg caatccgcat
     6901 cgcgacgatc ctgcgtcctg ctacatcgag acgctgacgc tcggcgacga gaagctcgag
     6961 cgctatcagc agttgacctc ggcgacacag aacacgaatc ggcccgcccg cgcggcgagc
     7021 ggcgagccga acatctatgg cgattgggcc caagagcagt acttgatcgc gcgcccgccg
     7081 gagggccgcg gcggtctcgt cccgaagagc atggtcgccg gcatcgagtc cgggcagatc
     7141 aagatcgccg acgtgccggg cgccggctgg ggtgcgagca ctgtgacgtt cacgccggcg
     7201 ggccaagcgg ccgccgacgc gctgcgcgcg cgaccgccgg atcagaacga gcgcgcgcgt
     7261 tgcgagatca cgagcgtgct attcgactgg gtgttcgacg gccccgtcaa tcgcatcacg
     7321 aagggcgccg atagcgtcac gatggaatac ggccgcggtc tcaagcgcac gatctacacg
     7381 a
//
LOCUS       DATLEE010000511         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5800054, whole genome shotgun sequence.
ACCESSION   DATLEE010000511 DATLEE010000000
VERSION     DATLEE010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5800054"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VM692_11015"
     CDS             <1..381
                     /locus_tag="VM692_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HUQ52742.1"
                     /translation="RRTLTSDRGIEMASHERFSLATQMQVYFCYPYSPRQRGSNENTN
                     GLLRQYFPKRADLSVHSQEHLDMIAARLNGRPRQTLNWHCPAEALDATVVASTGRKRT
                     CAPTVWEGLLRRRPRNCDAGRPTE"
     gene            complement(401..>2129)
                     /locus_tag="VM692_11020"
     CDS             complement(401..>2129)
                     /locus_tag="VM692_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HUQ52743.1"
                     /translation="SGVVVEPLLTDQWYVRAKPLAEPAIAAVEDGRVKFVPENWSKTY
                     FEWMHNIQDWCISRQLWWGHRIPAWYDDAGNIYVGRDEADVRRKHGLTASIALRQDED
                     VLDTWFSSALWPFSTLGWPDETPELKAFYPTNVLVTSFDIIFFWVARMLMMGLKFIGD
                     VPFREVYIHALVRDHEGNKMSKSKGNILDPLDLIDGVDLETLLKKRTDGLMQTHLQAG
                     IEKVTRKEFPNGIEGVGTDALRFTFASLATTGRDARFDLARAEGYHRFCNKLWNASAY
                     VFSQLEGVDDGPAELGVADRWIRSRLSATIASVHESFATYRLDLIAQTLYDFAWHELC
                     DWYLELTKAVLTDPAADPARRRGAQRTLVDVLGSVLKLLHPLMPFVTEELWLEVAKRR
                     QYQSSTIMLERFPQASDFPTDTAAEDEIGWLKGFVGGIRQIRGEANLPRSATLQVRLA
                     DATDSDRARVERHASHLRKLSGLDRIELLEPGASVKGSATALLGAMRILVPLAGLIDV
                     GAERERLGKQLAKTHDDLGKVRRKLENQSFVANAPPDIVAKEQARIAELEQRAAQLDQ
                     QIARLAELT"
BASE COUNT          351 a          692 c          706 g          380 t
ORIGIN      
        1 cgtcgcacgc tcacctcgga tcgaggcatc gagatggctt ctcacgagcg cttcagtctg
       61 gcgacacaga tgcaggtcta cttctgctat ccctacagtc cgcggcagcg ggggtcgaac
      121 gagaacacga atgggctgct acggcagtac ttccccaagc gcgccgatct gtcggttcat
      181 tcacaagagc atctcgatat gattgccgct cggttgaacg gccggcctcg ccaaaccctg
      241 aactggcact gcccagccga agcactggat gcgaccgttg ttgcatcgac tggtcgaaaa
      301 cgcacctgtg cgccgacagt ctgggaaggc cttctgcgac gtcggccccg gaactgcgat
      361 gccggtcgcc caacggaatg agctcggctc gggtgacagc tcaggtgagc tcggcgaggc
      421 gggcgatttg ttggtcgagt tgggccgcgc gttgctcgag ctcggcgatg cgggcttgct
      481 ctttcgcgac gatgtcgggc ggcgcgttcg cgacgaagct ctggttctcc agcttgcggc
      541 ggactttgcc gaggtcgtcg tgcgtcttcg cgagctgctt gccgagccgc tcgcgctccg
      601 cgcccacgtc gatgagcccg gcgagcggca cgaggatccg catcgcgccg agcagcgccg
      661 tcgccgagcc tttgacgctc gcgcccggct cgaggagctc gatgcggtcg aggcccgaca
      721 gcttgcgcaa gtggctcgcg tggcgctcga cgcgtgcgcg gtccgagtcc gtcgcgtcgg
      781 ccaagcgcac ctgcagcgtc gcggaccgcg gcaagttcgc ttcgccgcgg atctgccgaa
      841 tgccgccgac gaatcccttg agccagccga tctcgtcctc ggccgcggtg tcggtcggaa
      901 aatcactcgc ttgcgggaac cgctcgagca tgatcgtcga gctttgatac tggcgccgct
      961 tggcgacctc gagccagagc tcttcggtga cgaacggcat caacggatgc agcaacttga
     1021 gcacgctgcc gaggacgtcg acgagcgtgc gttgcgctcc gcggcgccgc gcgggatccg
     1081 cggccgggtc ggtcagcacg gctttcgtca gctcgaggta ccagtcgcag agctcgtgcc
     1141 aggcgaagtc gtacagcgtt tgcgcgatca ggtcgaggcg gtaagtcgcg aagctctcgt
     1201 gcaccgacgc gatcgtcgcg gagagacgcg accgaatcca gcgatcggcg acgccaagct
     1261 cggccgggcc gtcgtcgacg ccctcgagct ggctgaacac gtacgcagac gcgttccaca
     1321 gcttgttgca gaagcggtga tagccctccg cgcgtgcaag atcgaagcgc gcatcgcggc
     1381 ccgtcgtcgc tagcgacgcg aacgtgaagc gcaaggcatc cgtgccgacg ccttcgatgc
     1441 cgttcggaaa ctccttgcgc gtgaccttct cgatgccggc ctgcagatgc gtctgcatga
     1501 ggccgtcggt gcgcttcttc agcagcgtct cgaggtcgac gccgtcgatg aggtcgagcg
     1561 ggtccaagat gttgcctttc gacttcgaca tcttgttgcc ctcgtggtcg cgcacgagcg
     1621 catggatgta cacctcgcgg aacggcacgt cgccgatgaa cttgagcccc atcatcagca
     1681 tgcgcgcgac ccagaagaag atgatgtcga agctcgtcac gagcacgttg gtcgggtaaa
     1741 aggccttcag ctccggcgtc tcgtccggcc agcccagcgt cgagaagggc cagagcgccg
     1801 acgagaacca cgtgtcgagc acgtcttcgt cttggcgcaa ggcgatgctc gccgtgaggc
     1861 cgtgcttgcg ccgcacgtcg gcttcgtcgc gcccgacgta gatgttgccg gcgtcgtcgt
     1921 accaggccgg gatgcgatga ccccaccaga gctggcgcga gatgcaccaa tcctggatgt
     1981 tgtgcatcca ctcgaaatag gtcttcgacc agttctcggg cacgaatttg acgcgcccgt
     2041 cctcgacggc cgcgatcgca ggctcggcga gcggcttggc gcgcacgtac cactggtcgg
     2101 tgagcagcgg ctcgacgacg acgccgctg
//
LOCUS       DATLEE010000512         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2077987, whole genome shotgun sequence.
ACCESSION   DATLEE010000512 DATLEE010000000
VERSION     DATLEE010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077987"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VM692_11025"
     CDS             complement(<1..496)
                     /locus_tag="VM692_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52744.1"
                     /translation="MTRRITTAVVAALLCWPLLVVAAPHAVETAAPVPIERPDKPTGP
                     IVVEHRLLARPAVGVPLRIAVTARVEGDVGRLSIEANATSPSSVVVSPAVPVASTDGV
                     YAWEITVVPLTPDAGYLSVIVSGSIDGVAQARSVTVALRSAVPTEPAAVTVVEGETLI
                     ALPVQ"
     gene            complement(493..2223)
                     /locus_tag="VM692_11030"
     CDS             complement(493..2223)
                     /locus_tag="VM692_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52745.1"
                     /translation="MARWLLLALLATALAGCGGGSSSADPAPAPATGPVSVSGQVSFD
                     IVPAVEGQGLSYAATVARPARGVTVEMLQNGAVMASTATDATGNYSFANVAANTEIAL
                     RVRAELLRIGAPSWDFRVVDNVNADALYTLAGTVFNTGTTNVTRNLHAASGWGGSSYT
                     ATRSAAPFAILDLVYDAVQLVLSAAPDTAFPALRLHWSPANVPVVGTAAGEIGSSRYR
                     PSSGIYLVGAANQDTDEYDRHVIAHEFGHYLEDRFSRSDSFGGAHALTDQLDMRVAFG
                     EAWGSAFSAMVTGQEVYTDTHGPGQARWFSFNLEQSSSRVGPNANPNPGWFNEESLQS
                     LIYDLYDNGRDVPPGTLTVDDLALGFGPIWTVLTNEQRSTRALTSVFPFINALRTVRP
                     ADGPLIDQLTASQRIANVRDDYGSGQTNFGVPTQRTPAEVSADFHSIYDPLVVGGTLS
                     DVCSLNDYKSASTDAENKLASRRFVRFTVVNPGVHVITARAKAPLKSAADPDMLLHFG
                     DGQTLESSDAPTSTCTTATPGECVERFSPTLETGDYVLEVYEWTNTNRSDDATYPPIG
                     RTCFDVTVTR"
     gene            2426..>2590
                     /locus_tag="VM692_11035"
     CDS             2426..>2590
                     /locus_tag="VM692_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUQ52746.1"
                     /translation="MTHKTILVTGGAGYIGSHVVRQLGEAGEKVVVLDDLSTGFRQAV
                     LHGDLVVGNTG"
BASE COUNT          458 a          830 c          862 g          440 t
ORIGIN      
        1 cctgcacggg cagtgcgatc agcgtctcgc cttcgacaac cgtcacagcc gcgggctccg
       61 tggggacggc gctgcgcaag gcgacggtca cgctgcgcgc ctgggcgact ccgtcgatcg
      121 agcccgagac gatgacactc aagtatccgg catccggcgt cagcggcacg accgtgatct
      181 cccacgcgta gacgccgtcg gtcgatgcga ccggcacggc cggagacacg acgaccgagc
      241 tcggcgacgt tgcgttcgcc tcgatgctga ggcgaccgac gtcgccctcg acgcgcgcag
      301 tgacggcgat tcggagcggc acgccgacgg ccggccgcgc cagcaagcgg tgctcgacga
      361 cgatcgggcc cgtcggctta tcgggccgct cgatgggcac cggcgctgcc gtctcgacgg
      421 cgtgcggcgc ggccacgacc agcagcggcc aacagagcaa tgcggcgacg acggccgtag
      481 tgattcgcct cgtcatcgcg tcaccgtcac gtcgaagcat gtgcggccga tcggcggata
      541 cgtcgcgtcg tctgagcggt tcgtgttggt ccactcgtag acctcgagca cgtagtcgcc
      601 agtctcgagc gtcggcgaga acctctcgac gcattcgccg ggcgtcgccg tcgtgcaggt
      661 cgaggtcggc gcgtcgctcg actcgagcgt ctggccatcg ccgaagtgca gcagcatgtc
      721 gggatcggcc gcgcttttca gcggtgcctt cgcgcgcgcc gtgatgacgt gcaccccggg
      781 gttcacgacc gtgaagcgaa cgaagcggcg cgaggcaagc ttgttctcgg catcggtgct
      841 cgcactcttg tagtcgttca agctgcagac gtcggacaac gtcccaccga cgaccagcgg
      901 gtcatagatc gaatgaaaat ccgcagagac ctccgccggc gtacgttgcg tcggaacgcc
      961 gaaattagtc tggccggagc cgtaatcgtc tcggacattc gcgatacgtt gggatgccgt
     1021 caattggtcg atcaacggcc catccgccgg gcgcaccgtg cgaagagcgt tgatgaacgg
     1081 gaacacactc gtcagcgcac gcgttgagcg ctgctcgttc gtgagaactg tccaaattgg
     1141 cccgaaaccg agcgcaaggt cgtcgactgt caacgtgccg ggcgggacgt cgcgcccgtt
     1201 gtcataaagg tcatagatca atgactgaag tgattcttcg ttgaaccaac ccgggttggg
     1261 gttagcgttt ggccctacgc gactcgaact ttgttcaagg ttgaagctga accatcgtgc
     1321 ctgtcccggg ccgtgcgtat ccgtgtaaac ctcttgccct gtgaccatcg ccgaaaaagc
     1381 gctgccccag gcttcgccga acgcaacgcg catgtcgagc tgatccgtca acgcgtgtgc
     1441 gccgccgaaa ctgtcggagc gcgagaaccg atcctcgagg tagtgcccga actcgtgggc
     1501 gattacgtgc cggtcatact cgtcggtgtc ttgattggcg gcgccgacaa ggtagatccc
     1561 actgctcggc cgatagcggc tcgaaccgat ctcgccggct gcggtgccga cgacgggcac
     1621 attggccggg ctccagtgca agcgcaacgc cgggaacgcg gtatccggtg cggccgagag
     1681 gacgagctgc acggcgtcgt agacgagatc cagaatcgcg aacggcgccg cggagcgagt
     1741 cgccgtgtag gagctcccgc cccaacccga cgctgcatgc agattgcgcg tcacgttcgt
     1801 ggtgcccgtg ttgaacaccg tgccggcgag cgtgtagagc gcgtcggcgt tgacgttgtc
     1861 gacaacgcga aagtcccagc tcggcgcccc gatgcgtaac aactcggccc gcactcgcaa
     1921 cgcgatctcg gtgttcgcgg cgacgttggc gaaggagtaa ttgccggtcg catcggtggc
     1981 ggtgctcgcc atgacggcgc cgttttgcag catctccacc gtgacgccgc gcgcggggcg
     2041 tgcgaccgta gcggcgtagc tcaagccctg cccctcgacg gcagggacga tatcgaagct
     2101 gacttggccg ctcacgctaa cggggcccgt cgcgggagcg ggagccggat cggcactgct
     2161 gctgccgcca ccgcacccgg cgagcgctgt cgcgagcaaa gccaagagca accaacgagc
     2221 catggtccgt gatcctaacc gcgttacgac gagctgacag tgtctgtgtc tcgcgacgag
     2281 gagacgtcgg caaccgtctg cggccagcat tcgacgcttc cgtgaagcgg gagccgacgt
     2341 gtcgccaaat tgcgaacagt ttccgggctc ccgcgggggg agctgttaaa ctcagggccc
     2401 aagattatgt cgcgcattta agcctatgac gcacaaaaca attctcgtca cggggggagc
     2461 cggctacatc ggctcgcacg tcgttcgcca gctcggcgag gccggcgaga aggtggtcgt
     2521 gctcgacgat ttgtcgacgg gctttcgtca ggccgtgctg catggcgacc tcgtcgtcgg
     2581 caatacgggc
//
LOCUS       DATLEE010000513         5485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10734358, whole genome shotgun sequence.
ACCESSION   DATLEE010000513 DATLEE010000000
VERSION     DATLEE010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10734358"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VM692_11040"
     CDS             complement(<1..402)
                     /locus_tag="VM692_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HUQ52747.1"
                     /translation="MRAAAGNDVILEELELDALTELVNLGVSRAAANLAVMVQEEVLL
                     TVPKVELLTRDAAIRTLGEREARSLVAVHQTFEGAIVGRALLIFPEANSLELVRAIVG
                     SELSNEDIVELEQEALAETGNIILNSCLATIA"
     gene            complement(399..782)
                     /locus_tag="VM692_11045"
     CDS             complement(399..782)
                     /locus_tag="VM692_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUQ52748.1"
                     /translation="MVYKLLIVDDSKLARMAVVKALQSCHPDWQRVEAGNADDALRTA
                     QRDPPDIALIDFNMPNKNGLDLAADLRALSPDMPIGIISANHQQQVIDRARSLGAVFL
                     PKPLTEKALREFLATASQQLQGVPS"
     gene            complement(1071..3248)
                     /locus_tag="VM692_11050"
     CDS             complement(1071..3248)
                     /locus_tag="VM692_11050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HUQ52749.1"
                     /translation="MDELLKQFVIESRELVDKAVEDLLALEKAPNDSHRFEDVFRAFH
                     TLKGGAGIVEFGAMQQALHGAEDALAAARAESRPVSAPDVGNCLSCLDQVVEWLDAIE
                     RGGAIPIDADSADIVALFARAGAAKRAAGTVPSTARNGWQAELLDAHRAVAARARTAL
                     RYTPATDSFFRQHDPLAQIAELQGLLAVGLALREPSPQLEDLDPFSCNLTITALVAAS
                     PPDVTAALADEARHCEIAEVSKQPRAPGGESVARKGRELLEAQLRLLETTKERRQQGR
                     VASAGLVATNVLRHLGRRAEADAVATAAAVAIAEDRLEPLAEAIGAALNPVPSAGAAE
                     PRRAQGSQTIRVSSERIDALVRLTGELIVAKNALQHSVKLADQATAFGVTLKNQHAAL
                     ERLVNELQRAVVGIRVLPLRAAFQRFPRLVREMSAELQKPATLVIEGEDTEADKAIVE
                     ALVEPLVHVLRNAVDHGVEDAAARAEAGKPAVATIRLTAARKGEHVVIEVTDDGRGID
                     VARVKQVARERELVAPEAVVAMSDEEALDLIFLPGFSTADTVTQLSGRGVGMDAVRTA
                     VSRLGGQVEVRTALGTGTTVRVTLPFSVLVTEVMTVEAGGQVFGIPLDAVVETLRVED
                     GGIFPIGAAHAIVVRDRTIPLIRLADLLGGAPARGKGSGPIVIAQLEGELGAVQVDRI
                     GERMDIILKPLDGLLTGSRGLAGSALLGDGSVLLVLDLGEFVQ"
     gene            complement(3241..3633)
                     /locus_tag="VM692_11055"
     CDS             complement(3241..3633)
                     /locus_tag="VM692_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUQ52750.1"
                     /translation="MADDAPKRVLVVDDANLVRMYYRSALENAGFEVAEALNGLEALE
                     QLLTGHVDLVVVDVNMPRMDGLSFLQTLRAKSLPLSSIPALVTSTEAAPQRIAAAQLA
                     GANFYIVKPLRAETLVEYASLLCGVGHG"
     gene            complement(3637..4461)
                     /locus_tag="VM692_11060"
     CDS             complement(3637..4461)
                     /locus_tag="VM692_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HUQ52751.1"
                     /translation="MTQDEFRRLCEFLYRRTGMIFAEPKRYYVERRVQDRMLSTQSDS
                     FESYFSRLRADVHGEIEQFVNAFTVNETYFFREEHQLLCLTSDLLGARQQHKPRGASI
                     RIWSVPCSTGEEPYSIAIWLLEHWPHVDEYDIEIVGSDIDTAAVAMAREGVYGRRALT
                     RLPPRLIDKYFTADASGTSWQILDDLRQSVRFTAANIVNRAETRAHGQFDVIFCRNVL
                     IYFDDAARRTAAENLFENLLPGGFICLGHTESMSRISPLFEVARFADAIVYQRPME"
     gene            complement(4487..5107)
                     /locus_tag="VM692_11065"
     CDS             complement(4487..5107)
                     /locus_tag="VM692_11065"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUQ52752.1"
                     /translation="MPLIRKPPSEPPAAAARAPDAAAGARALQHGTDDERWSAARAAA
                     ELEIGVPTLVEALTRERVPAVREALFSALVSIASPQSVAAILPFLRSEDATVRTEASD
                     AVLAVKDAAWPHVPALLRDENPDVRILACGFARDMPRQAAVSLYCDLLDADPEANVCA
                     AVAEALAEIGDAAALPALLRCAERFRATPFVAYSLGVAIDRIRSQA"
     gene            complement(5091..>5485)
                     /locus_tag="VM692_11070"
     CDS             complement(5091..>5485)
                     /locus_tag="VM692_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HUQ52753.1"
                     /translation="VEAGNVYIGRGDADLIVTRRRAGVLAMSAPAERGYPWHPSANRL
                     VTSALEHVPPGQLIGVLMTGMGNDGAAAMTRLHELGGATIAESKDTAVVWGMPGELVE
                     AGGADWTEPVQKIAARLLKLVPTHAADS"
BASE COUNT          910 a         1894 c         1769 g          912 t
ORIGIN      
        1 cgcgatcgtc gcgagacagc tgttcagaat gatgttgccc gtttcggcca gcgcctcttg
       61 ctcgagctcg acgatgtcct cgttcgacag ctcgctgccg acgatcgccc ggacgagctc
      121 gaggctgttc gcctcgggaa agatcagcaa cgcccggccg acgatggcgc cctcgaacgt
      181 ctgatggacg gccaccaagc tgcgcgcttc gcgctcgccg agcgttcgga tcgcggcgtc
      241 gcgcgtcagc agctcgacct tcggaaccgt caacaagact tcttcctgaa ccatcactgc
      301 gaggttcgcg gccgcacggc taacgccgag gttcacgagc tccgtcagag cgtcgagctc
      361 gagctcctcc aaaatgacgt cgttgccggc agccgccctc acgacggcac gccttgcaat
      421 tgctgcgacg cagtggccaa gaattcgcgc agcgcttttt ccgtcaacgg cttgggcagg
      481 aacaccgcgc cgagtgagcg cgcgcgatcg atgacctgtt gttgatggtt cgccgagatg
      541 atgccgatcg gcatatcggg gcttagagcg cgcaaatccg cggccaggtc caaaccgttc
      601 ttgttcggca tgttgaaatc gatgagcgcg atgtccggcg gatcgcgttg ggccgtccgc
      661 agtgcgtcgt cggcattgcc ggcctctacg cgctgccagt cggggtggca gctctgcagc
      721 gctttgacca ccgccatgcg cgccagcttg ctgtcgtcga cgatcaggag cttgtagacc
      781 atcctgtatt tctccttcag agcgcaccac ggcgcgcgct aaccactatt ttctccgccc
      841 catcaacaac ggcgctatat gagcactcag aaacccgtcc ttgggtgctc cttggagacg
      901 aggttcgccc gccagcagta cttggacgac cgcggcatgg aggtactcgc agtccgtcca
      961 ttctacgggc gagccgggcc ctgccaagag gtgacgctgt aatacctcgg cctcctcgac
     1021 gccgcaccgg cccgtgagct cgatggtgcc tgtctgcgtg acacgtatgg tcattgcacg
     1081 aactcaccca ggtcgagcac cagtaacacg ctgccgtcgc cgagcaacgc ggagccggca
     1141 agcccgcgag aaccggtcaa caagccgtcg agcggcttca agatgatatc catgcgttcg
     1201 ccgatcctgt cgacctgcac ggcaccgagc tcgccttcga gctgcgcgat cacgatcggc
     1261 cccgacccct tgccgcgagc cggcgcgccg cccaggagat cggcgaggcg tatcaacgga
     1321 atcgtgcgat cgcgcacgac gatcgcgtgc gcggcgccga tgggaaagat gccgccgtct
     1381 tcgacgcgca gcgtttccac gacggcgtcg agcggaatgc cgaacacctg gccgcccgcc
     1441 tcgaccgtca tgacctcggt caccagcacg ctgaacggca aggtcaccct cactgtggtg
     1501 cccgtgccga gggcggtacg cacctcgacc tggcctccga gccggctcac ggccgtgcgc
     1561 acggcgtcca ttcccacgcc gcgccccgag agctgtgtga cggtgtccgc tgtcgagaag
     1621 cccggcagga agatcaggtc gagcgcttct tcgtcgctca tggcaacgac ggcttcgggc
     1681 gccacgagct cccgctctct cgctacctgc ttcactcgtg cgacgtcgat cccgcggccg
     1741 tcgtcggtca cctcgatgac gacgtgctcg cctttgcgag ctgccgtcaa ccggatcgtg
     1801 gcgacggcag gcttgccggc ctcggccctc gcagcggcat cttcgacgcc gtggtcgacg
     1861 gcgttgcgca gcacgtgcac gagcggctcg acgagcgctt cgacgatcgc cttgtcggcc
     1921 tccgtgtcct cgccctcgat tacgagcgtt gcgggcttct gcagctcggc cgacatctcc
     1981 cgtaccaggc gcgggaaccg ctggaacgct gcgcgcaacg gcagcacgcg gatgccgacc
     2041 acggcacgtt gcagctcgtt cacgagccgt tcgagcgcgg cgtgctgatt cttcaaggtg
     2101 acgccgaaag cggtcgcttg atcggccagc ttcaccgaat gctgtagcgc gttcttggcg
     2161 acgatgagct cgccggtcag gcgcacgagc gcatcgattc gttccgagct gacgcggatc
     2221 gtctgcgagc cctgcgcgcg acgcggctcg gcggcgccag ccgacggaac aggattcagt
     2281 gccgcgccga tggcctccgc gagcggctcg aggcgatctt cggcgatcgc cacggcggcc
     2341 gcggttgcga cggcgtcggc ctcggccctg cgaccgaggt gccgcagcac gttcgtcgcg
     2401 acgaggccgg ccgacgcaac tcgaccttgt tggcggcgct ccttcgtcgt ctcgagcaag
     2461 cgcagctgtg cctcgaggag ctcgcgcccc ttgcgcgcaa cggattcgcc gccgggcgcg
     2521 cgcggctgct tcgacacctc cgcgatctcg cagtgccgcg cctcgtccgc gagcgctgcc
     2581 gtcacgtcgg gcggcgacgc agcgaccaat gccgtgatcg tcagattgca cgaaaacggg
     2641 tcgaggtcct cgagctgcgg cgacggctcg cgcagcgcga ggccgacggc cagcaagccc
     2701 tgcagctcgg caatctgtgc gagcggatcg tgctgccgaa agaagctgtc cgtcgcgggc
     2761 gtgtagcgca gcgccgttcg tgctcgggca gcgacggcgc gatgcgcgtc gagcaactcc
     2821 gcctgccaac cgttgcgcgc agtcgacggc accgtgccgg cggcgcgctt tgccgctccg
     2881 gctcgcgcga acagcgcgac gatatcggct gaatccgcgt cgatggggat tgcgccgccc
     2941 ctctcgatcg cgtcgagcca ctcgaccact tggtcgaggc agctcaagca attgccgacg
     3001 tcgggcgcgg agacgggccg cgactcggcg cgcgcggccg cgagggcatc ctcggcgccg
     3061 tgcagcgcct gctgcatcgc gccgaactcg acgatgccgg caccgccttt caacgtatgg
     3121 aaagcgcgaa acacatcctc gaagcggtgc gaatcgttgg gcgccttctc gagcgcgagt
     3181 aaatcctcga cggccttgtc gacgagctcg cgtgactcga tcacgaattg cttcaacagc
     3241 tcatccatga ccgacaccgc acagtaacga cgcatactcg acgagcgtct cggcgcgcag
     3301 cggcttgacg atgtagaagt tcgccccggc cagctgcgct gcggcgatgc gctgcggcgc
     3361 cgcctctgtg ctcgtcacca acgccgggat cgacgacaac ggtaaacttt tcgccctcag
     3421 cgtctgcaag aagctgagcc cgtccatgcg cggcatgttg acgtcgacga cgaccagatc
     3481 gacatggccg gtgagcagct gctcgagcgc ttcgagcccg ttcagcgcct ccgcgacctc
     3541 gaaccccgcg ttttcgagcg ccgagcggta atacatgcgc acgagattcg cgtcatcgac
     3601 gaccaacact cgtttcggcg cgtcgtcagc cacgtgtcac tccatcggcc tctgatacac
     3661 gatcgcgtcg gcgaaacgcg ccacttcgaa gagcggcgag atgcggctca tcgactccgt
     3721 atgcccgagg cagatgaacc ctccgggcaa caggttctcg aacagattct cggccgcggt
     3781 tcgccgagcc gcgtcgtcga aataaatcag gacgtttctg cagaagatga cgtcgaactg
     3841 cccgtgggct cgggtctcgg cacggttgac gatgttcgcc gcagtgaaac gcaccgactg
     3901 gcgcaagtcg tcgagaatct gccaactcgt gccggaagcg tcggccgtga agtacttgtc
     3961 gatgagccgc ggcggcaacc gcgtcaacgc gcgtcgaccg tagacgccct cgcgcgccat
     4021 cgcgaccgcc gcggtgtcga tgtccgagcc gacgatctcg atgtcgtatt cgtcgacgtg
     4081 cggccaatgc tcgagcagcc agatcgcgat cgagtaaggc tcttcgcccg tggagcacgg
     4141 cacggaccag atgcggatcg acgcgccgcg cggcttgtgc tgctgtcgcg cgcccagcag
     4201 gtcggacgtc aggcaaagaa gttgatgctc ttcgcgaaag aagtacgttt cgttgaccgt
     4261 gaacgcgttg acgaactgct cgatctcgcc atggacatcg gcgcgcaagc gcgaaaagta
     4321 gctttcgaac gagtcggact gcgtgctcag cattcggtct tgcacgcgcc gctcgacgta
     4381 atagcgtttc ggttcggcaa agatcattcc cgttcggcgg tagaggaact cgcacaacct
     4441 gcgaaattcg tcctgagtca gtgccgcgag ctgagccacg ggggtcctaa gcttgcgagc
     4501 ggatacggtc gatcgcgacg cccagcgaat acgcgacaaa cggcgtcgca cggaatcgct
     4561 cggcgcagcg cagcagagcc ggcagcgccg cggcgtcgcc gatttcggcc agcgcttcgg
     4621 cgaccgcagc gcatacgttg gcttccgggt cggcatcgag caggtcgcaa tacaggctca
     4681 cggctgcctg ccgcggcatg tcgcgcgcga acccgcacgc gagaatccgg acgtccgggt
     4741 tctcatcgcg caacaaagcc ggcacgtgcg gccacgccgc gtctttcacc gcgagcacgg
     4801 cgtccgacgc ctcggtgcgg acggtggcat cctcggagcg caggaacggc aggatcgctg
     4861 cgacgctctg cgggcttgcg atgctcacga gcgccgagaa cagcgcttcg cggacggccg
     4921 gcacgcgctc tcgcgtcaaa gcctcgacga gcgtcggcac gccgatctcg agctccgccg
     4981 cagcgcgtgc ggccgaccag cgctcgtcat cggtaccgtg ctgcagcgcg cgagcaccgg
     5041 cggccgcatc cggggcccgc gcagccgcgg ccggcggttc cgacggcggt ttacgaatca
     5101 gcggcatgag tcggcaccag cttcagcaac cgagcggcga tcttttgcac gggctccgtc
     5161 caatcggccc cacccgcctc gacgagctcg ccgggcatgc cccatacgac ggcagtgtcc
     5221 ttggactctg cgatggttgc gccgccgagc tcgtgcaggc gcgtcatcgc ggccgcgccg
     5281 tcgttgccca tgcccgtcat cagcacgcca atgagttggc cgggcggcac gtgctcgagc
     5341 gccgaggtga cgagccggtt cgcgctcggg tgccatggat agccgcgctc cgccggagca
     5401 ctcatcgcca gcacgcccgc gcggcggcgc gtcacgatca ggtccgcatc gccgcggccg
     5461 atgtagacgt tgccggcctc gaccg
//
LOCUS       DATLEE010000514         3549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5060079, whole genome shotgun sequence.
ACCESSION   DATLEE010000514 DATLEE010000000
VERSION     DATLEE010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5060079"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VM692_11075"
     CDS             complement(<1..265)
                     /locus_tag="VM692_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ52754.1"
                     /translation="MFGVSVRSLTKVTAALVAAAAFAAPVAAADDLASIAARVRVLED
                     REAIRALILAYGQAHDHRDYRTFASLFASNGEWVGGLGSAKGPE"
     gene            complement(292..1761)
                     /locus_tag="VM692_11080"
     CDS             complement(292..1761)
                     /locus_tag="VM692_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HUQ52755.1"
                     /translation="MSAVPQTNIEPSIKAPHSFFIGGKWQKPASNGKLSVISPVTEEV
                     VLTFAEATPQDVDRAVAAAREAFDRGPWPRMTAQERGAALLKVSELLKARQPELAQIW
                     TTQVGAAISFTRYVSGQPAELFEYYGKLIQSYPLVDERRRDNGGLVRVVKEPVGVVAA
                     ITPWNAPLVLLCYKVAAGLAAGCTIVAKPAPETPADAYILAECIEAAGLPPGVFNVIP
                     AGRETGDYLVRHPDIDKISFTGSTVAGRHIAAVAAERFVRTSFELGGKSAAIVLDDAD
                     VGQTLQSLVPYSMPITGQVCFSLTRVLVPEKRKQEFLDAYVAAVKTVKVGDPQDPATQ
                     MGPLAMKRQLERVQGYIGKGKAEGAKLVLGGGRPKGLDRGYFVEPTVFADVDQSMTIA
                     REEIFGPVVSFISYNGVDDALAKANATMYGLHGAVYTPNAERGYEVARRVRSGSVTVN
                     GMIVDPKMPFGGFKQSGWGREGGLEGLEPYFELKTVYFA"
     gene            complement(1820..2959)
                     /locus_tag="VM692_11085"
     CDS             complement(1820..2959)
                     /locus_tag="VM692_11085"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52756.1"
                     /translation="MRTSACLAVVAAALVVPVFPVLAHHSNSAYQVDQIVTLTGTVKE
                     WRWMNPHTWLLLTVQGEDGKTQEWAVEGRPPGILGRAGWNSTILVPGERVTVHASPAK
                     NGDPEAIIARVTKADGTVLANQPNFNREAASAAARPGAAAPVAAAGSRPSFAGVYYPA
                     GGEGGPAQAAARPANQPLPPPTRSSPTADGSQGRGPDAPKLTPPYLAKWNVLAKSRIS
                     GSYEYDNIANCLPPGVPAMMSAAYGMEIMQDEQKITIFSEHQDALRRIFLDGRKPSAQ
                     VLADPTYAGYSTGRWEGDTLVVDTVALTTKSYIDGSSPHSDKMTVQERLRFVEPGVLE
                     DQIVVTDPEALTEPWRTTRRYRKAAYPNDELREFACAEGLRDAPH"
     gene            3073..>3549
                     /locus_tag="VM692_11090"
     CDS             3073..>3549
                     /locus_tag="VM692_11090"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HUQ52757.1"
                     /translation="MADEQSTKKPPFEPMVAASNFLSAAEIDRLIEEHTPLLAEGKLG
                     AGATNAAIRRSRVVFLRMEERYRWLYERLWIAAQDCNRSFFGVDITTVEANVQLARYD
                     SSDKGFYDWHTDFAEFRPLRKLSFSVQLSASEDYEGGDLQLWVGNEPRAAVRERGAL"
BASE COUNT          543 a         1195 c         1199 g          612 t
ORIGIN      
        1 cttcggggcc cttcgccgat ccgagtccgc cgacccattc gccgttgctg gcgaacaggc
       61 tcgcgaacgt gcggtagtcg cggtggtcgt gcgcttgacc gtacgcgagg atcagcgcgc
      121 ggatcgcctc gcgatcctcg agcacgcgga cgcgtgcggc gatggacgcg agatcgtcgg
      181 cggcggcgac gggcgctgcg aatgcagcgg ccgcgacgag cgcggccgtg accttggtca
      241 gcgagcgaac agaaactccg aacatggtat ccccctgttg ccggctcgct gctaggcgaa
      301 gtacacggtc ttcagctcga agtacggctc gagaccctcg aggccgccct ctcgccccca
      361 acccgattgc ttgaagccgc cgaacggcat cttcgggtcg acgatcatgc cgttgaccgt
      421 aacgctgccg gaccggacgc gccgcgcgac ctcgtagccg cgctcggcgt tcggcgtgta
      481 gacggcgccg tgcaacccgt acatcgtcgc gttcgccttc gcgagcgcgt cgtcgacgcc
      541 gttgtacgag atgaacgata cgacgggccc gaaaatttcc tcgcgcgcaa tcgtcatcga
      601 ctgatcgaca tcggcgaaca cggtcggctc gacgaagtag ccgcgatcga gccctttcgg
      661 ccggccgccg ccgagcacga gcttcgcgcc ttcggcctta cccttgccga tgtagccttg
      721 cacgcgctcg agctgccgct tcatcgcgag cggccccatc tgcgtcgcgg ggtcctgtgg
      781 gtcgccgacc ttgacggtct tgacggcggc gacgtaagcg tcgagaaact cctgcttgcg
      841 cttttcgggc acgagcacgc gcgtcaacga gaagcagact tggcccgtaa tcggcatcga
      901 gtacggcacg aggctctgca gcgtctggcc gacgtcggcg tcgtcgagca cgatcgcggc
      961 cgacttgccg ccgagctcga agctcgtgcg cacgaagcgc tccgcggcaa ccgccgcgat
     1021 gtgccggccg gcgacggtcg agcccgtgaa gctgatcttg tcgatgtcgg ggtggcgaac
     1081 cagataatcg cccgtctcgc gcccggccgg gatcacgttg aagaccccgg gcggcaaccc
     1141 ggcggcctcg atgcactcgg cgaggatgta ggcgtcggcc ggcgtctcgg gcgcgggctt
     1201 cgcgacgatc gtgcaaccgg ccgcgaggcc ggccgcgacc ttgtagcaga gcagcacgag
     1261 cggcgcgttc catggcgtga tcgccgcgac cacgccgacg ggctccttga cgacgcgcac
     1321 gaggccgcca ttgtcgcggc ggcgctcgtc gacgagcggg tacgactgga tcaacttgcc
     1381 gtagtactcg aacagctcgg cgggctgccc cgatacgtag cgcgtgaagc tgatcgccgc
     1441 tccgacctgc gtcgtccaaa tctgcgcgag ctccggctgc cgcgctttca ggagctcgga
     1501 tactttcagc agcgcggcgc cgcgctcctg cgccgtcatg cgcggccacg gcccgcgatc
     1561 gaacgcttcg cgtgcggccg cgacggcgcg gtcgacgtcc tgcggggtcg cttcggcgaa
     1621 cgtcagcacg acctcttcgg tgaccggcga gatgacgctc aacttgccgt tgctcgcggg
     1681 cttctgccat ttgccgccga tgaagaaact atgcggcgct ttgatcgagg gctcgatgtt
     1741 cgtttgcggc acggcgctca tggacgtctc cttacgtttg tgtcgggcaa tgatagcgct
     1801 gtttgctgcg cagccccggc tagtgcggcg cgtcgcgcag gccttcggcg cacgcaaact
     1861 cgcgcagctc gtcgttcgga taagcggcct tgcggtagcg gcgcgtcgtg cgccatggct
     1921 ccgtcagagc ctcggggtcc gtgacgacga tctggtcctc gagcacgccg ggctcgacga
     1981 agcgcaagcg ctcctgcacc gtcatcttgt cgctgtgcgg cgatgagccg tcgatatacg
     2041 acttcgtcgt cagtgcgacc gtatcgacga cgagcgtgtc gccctcccaa cggcccgtcg
     2101 agtagccggc gtaggtcgga tcggccagca cttgcgccga cggcttgcgg ccatcgagaa
     2161 agattcggcg cagcgcgtcc tggtgctcgc tgaagatcgt gatcttctgc tcgtcctgca
     2221 tgatctccat gccgtacgcc gcgctcatca tcgcgggcac gccgggcggc aagcagttcg
     2281 cgatgttgtc gtactcgtac gagccgctga tgcgcgactt cgcgagcacg ttccacttcg
     2341 cgaggtacgg cggcgtcagc ttcggcgcat cggggccgcg gccttgcgag ccgtcggccg
     2401 tcggcgacga gcgagtcggg ggcggcaatg gttgattggc cggacgagcc gctgcctgcg
     2461 ccggtccgcc ctctccgccc gccgggtaat agacgcccgc gaagctcggc cgactcccgg
     2521 cagctgcaac cggcgcagcc gcccccgggc gtgcggcggc cgacgctgcc tcgcgattga
     2581 agttcggctg gttggcgagc acggtgccgt cggccttcgt gacgcgcgcg atgatcgctt
     2641 cggggtcgcc gttcttggcc gggctcgcgt gcaccgtcac acgctcgccg ggcacgagga
     2701 tcgtgctgtt ccagccggcg cggccgagga taccgggcgg ccgaccttcg acggcccact
     2761 cctgcgtttt gccgtcctca ccttgcacgg tgagcagtag ccacgtgtgc ggattcatcc
     2821 agcgccactc cttgacggtg cccgtcaggg tgacgatttg atcgacttga taagccgagt
     2881 tgctgtggtg cgccagcacg gggaatacgg ggacgacgag cgcggcggca accaccgcga
     2941 gacaggccga cgttcgcatg agatctcctg ttcgagtcga gccaaagctc cggctatcgt
     3001 ccgcgcggct cgcgtagagc gcaagcgttg cgccgtcggc tatattgcgg cgaaccgcaa
     3061 acgcacctag gcatggccga cgagcaaagt accaagaagc cgcctttcga gccgatggtg
     3121 gccgcctcga acttcctgag cgcagccgag atcgaccgct tgatcgagga gcacacgccc
     3181 ctcctcgcgg aaggcaagct cggtgccgga gccacgaatg ccgcgatacg gcgctcgcgg
     3241 gtcgtcttcc tccgaatgga ggagcgttac cgctggctct acgagcggct gtggatcgca
     3301 gcgcaagact gcaatcgcag cttcttcggc gtcgatatca cgaccgtcga agcgaacgtg
     3361 cagctcgcgc gctacgacag ctcggacaaa ggtttctacg actggcatac ggacttcgcg
     3421 gaatttcgac cgctgcgcaa gctgtcgttc tccgtgcagc tgtccgcgtc cgaggactac
     3481 gagggcggcg atctgcagct ctgggtcggc aacgagccgc gcgcggccgt gcgcgagcgc
     3541 ggagcgttg
//
LOCUS       DATLEE010000515         7325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2079578, whole genome shotgun sequence.
ACCESSION   DATLEE010000515 DATLEE010000000
VERSION     DATLEE010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079578"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1021
                     /locus_tag="VM692_11095"
     CDS             194..1021
                     /locus_tag="VM692_11095"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HUQ52758.1"
                     /translation="MSAVGERVQLFLGEHEREQADRSLPHGEVCVRSLRSPDKKTDNE
                     DSAAIIQLGDDSLVLAVADGVGGSVAGREASNAAVRTLSRLLTKLPEATTQLRPAILD
                     AVEAANKAVLNIARGAATTLVVAQLDATKLRSYHVGDSELLAVGQRGRIKQRVVPHSP
                     TGFAVEAGLLDEDEAVQHEQRHVLFNVIGSAEMRVEVGPALQLAARDTVLLASDGLFD
                     NLYIDEIVDTIRSGPLTEAADKLVARVQARMHGEDKASEPCKPDDLTVVLFRPHKPR"
     gene            complement(1065..2447)
                     /gene="tagH"
                     /locus_tag="VM692_11100"
     CDS             complement(1065..2447)
                     /gene="tagH"
                     /locus_tag="VM692_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HUQ52759.1"
                     /translation="MGLRLQIISRHRQGLGDRGSMEFGHNGGTIGRSLESDWVLPDGQ
                     RYLSSRHASIDFRSGSYYVVDTSTNGVYVNDAEQPVGRGNPQRLFTGDRLRIGEYEMS
                     VEIQELEDTRETLANDKHVDPVSRAQRVPPPDPTRADMVAPHEITAVGIEMLIDEDAE
                     IRQIQRHNKANVASLRLEEDVAPPTPKVAPPQPPPAAKQTEPEPVAAAPKRPEPPPVS
                     AAARVVHRPAAPASTPKPHINLSAPAKPAQPPAPPTPAAPAPSLEAFFRGAGLPAQRL
                     DDKQAEQVLHRLGQLMREVVLGVTENLHLRAEQKNVLRVPTTTIQPQNNNPLKFSASV
                     EEALNNLLFRDSAEYLSAVDAVRETFADIKQHQQHLLSALRTAVVDYVGRLDPDELEN
                     KISNGKHGLLNAANKLKYWDLFKDLYQVVSTHQPGQFPPQFLEELARAYELEESRTAS
                     PAASRQTKLG"
     gene            2616..2963
                     /locus_tag="VM692_11105"
     CDS             2616..2963
                     /locus_tag="VM692_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HUQ52760.1"
                     /translation="MAAKPIYRVVFLNQGKVYEVYARQVSQTGSLFGFVEIEELVFDA
                     RKGVVVDPSEERLRIEFAGVKRTYLPMHYVLRIDEVDKQGVSKITAAEGGNVTQFPMP
                     VYTPQGKGGPQER"
     gene            2991..3752
                     /locus_tag="VM692_11110"
     CDS             2991..3752
                     /locus_tag="VM692_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007236248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ52761.1"
                     /translation="MTLRFLALGSGSRGNAALVEFGSTLLMIDCGLPCKVVEERLQAV
                     DRQPRDITAILVTHEHTDHAQGVATFSKRHNTPVWMTVGTASAMPTITRVRHLSAHRS
                     LTIGSIDVQPYPVPHDAREPCQYTFAAAGRRLGMLTDAGHVTPHMVERLTDCDALALE
                     CNHDLESLQRGPYPESLKARVASKFGHLNNHQTTELLRRVDATRVQWLLGLHLSERNN
                     SAEHVRTTLQPALANARYPLHLASQDSPTAWLAVE"
     gene            4181..>7325
                     /gene="purL"
                     /locus_tag="VM692_11115"
                     /gene_synonym="purI"
     CDS             4181..>7325
                     /gene="purL"
                     /locus_tag="VM692_11115"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HUQ52762.1"
                     /translation="MLQLAGPPAATAFRIEKLRAELRSIAPTVTDVGVHFDHFVSLER
                     PLTTAEQRVLEALLDYGGGAVGRPSGRQKLYVVPRLGTISPWASKATDIAKICSLPVL
                     RIERGRVYELAVGGVLTDVQRKRIAPLVHDRMTETLLMQRPLEELLFAQHEPRPVRTI
                     DVLGGGRDAMMRANAEIGLALSADEIDYLVAQFSGLQRNPTDVELMMFAQANSEHCRH
                     KIFNADWIIDGQRAPKSLFAMIKNTHARSPAGVLSAYKDNAAVVVGADTAWLWPDAAT
                     GVYGYANEPAHLVMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLCGF
                     TVSHLELPGWRQPWERASPGKPDRIASPLAIMLDGPIGAASFNNEFGRPNLAGYFRTT
                     LLEANGVWRGYHKPIMIAGGVGNVRGANVVKGVPGPGAKLVVLGGPAMLIGLGGGAAS
                     SQGSGAGEAELDFASVQRGNAEMQRRAQEVIDACWALGDLNPIAAIHDIGAGGLSNAV
                     PEIVDHSNCGAEIDLRAVPNDEPGMSPMELWCNESQERYMLTIEAHDVERFAAICVRE
                     RAPYAVIGELTAKRELVVRDSQLGNTPVAMPMEVLFGKPPKMLRQAASSEPKAPEWRR
                     EQMSFAAAVDRVLAFPAVADKSFLIHIGDRTVGGLCSRDQLVGPWQVPVSDVAVTASG
                     YNGYTGEALAMGERTPVAIHDGPASARLAVAEAVLNIAAADVAALGDIRLSANWMAAA
                     GHADDDYALYAMVRAVGEELCPALGLAVPVGKDSLSMRTVWQDGGRERAVTAPVSLIV
                     SAFAPVVDIRRTLTPELDRDVDSVLVFVDLVGGAQRLGGSSLAQTFGQFGGAAPDVDD
                     QQRLVRWFAAQRALRAADLLLAYHDRSDGGLFTTLVEMAFAANVGLDVELDGGVADAI
                     AYLFNEELGAVIQVPSAQLSRVEQVLAEAQLEHRTIARIARHDDVVVRRAATELYRAP
                     RVELRRKWSELTYRMQALRDDPQCAREAFDAVLDAKDPGLNAKLTFPLPNERRAAARA
                     GRPKVAVLREQGVNGQRE"
BASE COUNT         1131 a         2428 c         2560 g         1206 t
ORIGIN      
        1 tgccgcgggc cgctgatgcc gttcatgcgc tactgtccat ggtgccgtgc caagaccaaa
       61 cgtccatgga agctcccggg cagcacgacg cgctgctcgc gctgccgttg gggcgtcgac
      121 actaattttt ggcactactg cccgtggtgc acgaaggcgc tcgaaatatg agtgccacgc
      181 aaggccgagc tccatgagtg cggtcggcga gcgcgtgcag ttgttcctcg gtgagcacga
      241 gcgcgagcaa gccgaccgat cgttaccgca cggcgaagtc tgcgtccgca gcctgcgctc
      301 gcccgacaag aagaccgaca acgaggactc cgctgcgatc atccagctcg gcgacgactc
      361 gctggtgctc gccgtcgccg acggcgtcgg cggctccgtt gcggggcgcg aggcgtcgaa
      421 cgcagcggtg cgcacgttgt cgcggctact gacgaagcta ccggaagcga cgacgcagct
      481 gcggcccgcg atcctcgatg ccgtcgaggc ggcgaacaag gcggtgctga acatcgcacg
      541 cggcgccgcg acgacgctcg tcgtcgcgca gctcgatgcg acgaagctgc gcagctacca
      601 cgtcggcgac tcggagctgt tggccgtggg ccagcgcggg cgaatcaagc agcgcgtcgt
      661 gccgcattcg ccgacgggct tcgccgtcga ggccggcttg ctcgacgagg acgaggccgt
      721 gcagcacgag cagcgccacg tgctgttcaa cgtcatcggc tcggcggaga tgcgcgtcga
      781 ggtcgggcct gcgctgcagc tcgcggcgcg cgacacggtg ctgctcgcga gtgacgggct
      841 gttcgacaac ctctacatcg acgagatcgt cgacacgatt cgcagcgggc cgctgaccga
      901 ggccgccgac aagctcgtcg cacgtgtgca ggcccgcatg cacggcgagg acaaagcgag
      961 cgagccgtgc aagcccgacg acctgacggt cgtgctgttc cggccgcaca agccgcgctg
     1021 acgcgcggcg cccgacgtca ctgcatgcgc ctaacgcgga ggcgtcagcc gagcttggtc
     1081 tgccgcgagg cggcgggcga ggcggtgcgg gactcctcga gctcgtaggc gcgcgcgagc
     1141 tcctcgagga attgcggcgg gaactgcccc ggctgatgag tcgagacgac ctgatagagg
     1201 tccttgaaca ggtcccagta cttgagcttg ttcgctgcgt tcagcagtcc gtgcttaccg
     1261 ttgctgattt tgttctcgag ctcgtcggga tcgagccggc cgacgtagtc gacgacagcc
     1321 gtgcgcagcg ccgagaggag gtgctgctga tgttgcttga tgtcggcgaa cgtctcgcgc
     1381 acggcgtcga cggccgagag gtactccgcg gaatcgcgga acagcaagtt gttcaacgct
     1441 tcctcgacgc tcgccgagaa cttcagcggg ttgttgttct gcggctggat cgtcgtcgtc
     1501 ggcacgcgca gcacgttctt ctgctcggcg cgcaagtgca ggttctcggt gacgccgagc
     1561 acgacttcac gcatcagttg gccgaggcgg tgcaagacct gctcggcctg cttgtcgtcg
     1621 agccgctggg ccggcaagcc ggcaccgcgg aagaacgcct cgagcgaggg cgccggcgcc
     1681 gccggcgtgg gtggagcggg aggctgcgcc ggttttgccg gcgccgagag gttgatgtgc
     1741 ggcttcgggg tcgaggccgg cgcggccggg cggtgcacga cgcgcgccgc cgcgctgacg
     1801 ggcggcggct caggtcgttt cggcgcggcg gctacgggct cgggctccgt ttgcttggcg
     1861 gccggcggcg gctgcggcgg cgcaaccttc ggcgtcgggg gagccacgtc ctcctccaga
     1921 cggaggctcg cgacgttggc cttgttgtgg cgctgaatct ggcggatctc ggcgtcctca
     1981 tcgatcagca tctcgatgcc gacggccgtg atttcgtgcg gtgcaaccat gtcggcccgc
     2041 gtcgggtccg gcggcgggac gcgctgcgct cgcgaaacgg gatcgacgtg cttgtcgttg
     2101 gcgagcgtct cgcgcgtgtc ctcgagctct tggatctcga cgctcatctc gtattcgccg
     2161 atacgaaggc ggtcgcccgt gaacaagcgc tgcggattgc cgcggccgac gggctgctcc
     2221 gcgtcgttga cgtagacgcc gttcgtcgaa gtgtcgacga cgtagtagct gccggagcgg
     2281 aagtcgatgc tcgcgtggcg gctcgataga taacgctgac cgtccgggag cacccagtcg
     2341 ctctcgagcg agcggccgat cgtgccgccg ttgtgcccga attccatcga gcctcggtcg
     2401 ccgaggcctt gacgatgacg actgatgatc tgcaggcgta ggcccatatc gatccctaaa
     2461 gaaccgggcc gcgcccggtc gacgtccgta tctccccccg cgcgcgagtc tagctccgaa
     2521 cctccgcaga atccgagagt tgcatgcgat tactgtgatc gggctctcac cagatcgtgt
     2581 gctaagctcc gcttgccctt tctggatgga cccgcatggc agcgaagccg atctaccgcg
     2641 tcgtttttct caatcaaggc aaggtctacg aggtctacgc gcggcaagtg agccagaccg
     2701 gctcgctgtt cggcttcgtc gagatcgaag agctcgtctt cgacgcgcgc aagggcgtcg
     2761 tcgtcgatcc ttccgaggaa cggttgcgca tcgagtttgc cggcgtcaag cgcacctatc
     2821 tgccgatgca ctacgtgctc cgcatcgacg aggtcgacaa gcagggcgtc agcaagatca
     2881 ccgcggcgga gggcggcaac gtcacgcagt tcccgatgcc cgtctacacg ccgcaaggca
     2941 agggcggacc gcaagagcgc tagatcgttg cgagccagcg gaacatcctc gtgaccctgc
     3001 gtttcctcgc gctcggcagc ggcagccgcg gcaatgcggc attggtcgag ttcggctcga
     3061 ccttgttgat gatcgactgc ggcttgccgt gcaaggtcgt cgaggagcga ctacaagccg
     3121 tcgatcgtca gccgcgcgac atcacggcca tcttggtcac tcacgagcac acggaccatg
     3181 cgcaaggcgt cgccacgttc tcgaagcgcc acaacactcc ggtctggatg acggtgggca
     3241 cggcgagcgc tatgccgacg atcacgcgcg tccgccattt gagcgcgcat cgatcgctga
     3301 cgatcggcag catcgacgtg cagccgtacc ccgtgccgca tgacgcacgc gagccgtgcc
     3361 aatacacgtt tgcggccgcg ggccggcgcc tcggcatgtt gaccgacgcg gggcacgtga
     3421 ctccgcacat ggtcgagcgg ctcacggact gcgatgcgct cgcgctcgag tgcaatcacg
     3481 acctcgagtc gctgcagcgc ggcccgtacc ccgagtcgct gaaggcgcgc gtcgcgtcga
     3541 agttcggtca cctgaacaat catcagacga cggagctgct gcgccgcgtc gacgcgactc
     3601 gcgtgcaatg gttgctcggg ttgcacttga gcgagcgcaa caactcggcg gagcacgtcc
     3661 gcacgacgct gcagcccgca ctagcgaacg cgcgttaccc gctgcatctc gcaagtcagg
     3721 attcgccgac ggcgtggctc gccgtcgagt agcagacgca gctcgcgtgc ccgtgcgagc
     3781 tcgagcacgc gctcgacgcg catcgccgga ccggccactg cagccgcgcc ctccgcgacc
     3841 ccggcgatac gctccaggtt ctcgttagtt tgccgttgcg cttctgcggc ctgcgcgatc
     3901 gcggcagcct gttgcgctgc cgccgccgct cgatcggcac ccggaccgac gcccgtggcg
     3961 aggccgcgca tcgcctggcc gaaacgggga cgtgacggcg ccgtctgtta ctcagcggcc
     4021 tgctgagctt cctgcaccgt cgcgaggtct cgacagcgac gtccgtggac gcgcccgtct
     4081 cggcgcgtac caagtggcag ggccgatggg cgcaagagta gaatgctcgc gccgcagagc
     4141 tcgccttaga gctcctctga cgtgagatga acccatgacg atgttgcagt tggccggtcc
     4201 cccggcagcg actgcgtttc gaatcgaaaa gcttcgtgcc gaattgcgct cgatcgctcc
     4261 gacggtgacc gacgtcggcg tgcatttcga tcacttcgtg agcctcgagc ggccgcttac
     4321 gacggcggag cagcgtgtgc tcgaggcatt gctcgactac ggcggcgggg cagtcgggcg
     4381 gccgagcggc cggcagaagc tctatgtcgt tccgcggctc gggacgatct cgccgtgggc
     4441 ttcgaaggcg accgacatcg caaagatctg ctcgctgccg gtgcttcgaa tcgagcgcgg
     4501 ccgcgtttac gagcttgccg tcggcggcgt gctgacggac gtccagcgca agcgcatcgc
     4561 gccgctggtg cacgatcgca tgacggagac gctgctgatg cagcggccgc ttgaggagct
     4621 tttgttcgcg cagcacgagc cgcggcccgt gcgcacgatc gacgtgctcg gcggcggccg
     4681 cgacgccatg atgcgcgcca acgccgagat cggcctcgcg ctgtcggcgg acgagatcga
     4741 ctatctcgtc gcacagttca gcggcctgca gcgcaatccg accgacgtcg agctcatgat
     4801 gttcgcgcaa gcgaactccg agcactgccg gcacaagatc ttcaacgccg actggatcat
     4861 cgacggccag cgcgcgccga agagcctgtt cgcaatgatc aagaacacgc acgcgcgctc
     4921 gccggccggc gtgttgtcgg cttacaagga caacgctgct gtcgtcgtcg gcgcggacac
     4981 tgcgtggctg tggcccgatg ccgcgaccgg cgtctacggc tacgcgaatg agccggcgca
     5041 cctcgtcatg aaggtcgaga cgcacaacca tccgacggcg atctcgccgt tccccggcgc
     5101 cgcgacgggc tcgggcggcg agatccgcga cgaaggtgcg acgggccgcg gtgcgaagcc
     5161 gaaagcgggg ctctgcggct tcacggtttc gcacctcgag ctgccgggtt ggcgccagcc
     5221 gtgggagcgg gcgtcgccgg gtaagccgga tcgcatcgcg tcgccgctcg cgatcatgct
     5281 cgacgggccg atcggcgcgg cgagcttcaa caacgagttc ggccggccta acctcgccgg
     5341 ttatttccgc acgacgttgc tcgaagcgaa cggcgtttgg cgcggctatc acaagccgat
     5401 catgatcgcc ggcggcgtcg gcaacgtccg cggcgccaac gtcgtcaaag gtgtgccggg
     5461 ccccggagcc aagctcgtcg tgcttggcgg gcccgcgatg ttgatcggcc tcggtggcgg
     5521 cgcggcctcg tcgcagggca gcggcgccgg tgaagccgag ctcgatttcg cgtccgtgca
     5581 gcgcggcaac gccgaaatgc agcggcgtgc gcaggaagtc atcgacgcgt gttgggcgct
     5641 tggggacttg aacccgatcg ccgcgatcca tgacatcggc gccggcggcc tgtcgaacgc
     5701 cgtgcccgag atcgtcgacc acagcaactg cggcgcggag atcgatctgc gcgccgtgcc
     5761 gaacgacgag cccggcatga gcccgatgga gctctggtgc aacgagtcgc aagagcgcta
     5821 catgctgacg atcgaggcgc acgacgtcga gcgcttcgcg gcaatctgcg ttcgcgagcg
     5881 agcgccgtac gccgtcatcg gtgagctcac ggcgaagcgc gagctcgtcg tgcgtgattc
     5941 acagctcggc aacacacctg ttgcgatgcc gatggaagtg ctgttcggca agccgccgaa
     6001 gatgctgcgg caggctgcga gctcggagcc aaaggcgccc gagtggcggc gcgagcaaat
     6061 gagcttcgcg gcggccgtcg atcgagtgct ggcgtttccg gccgtcgccg acaagtcgtt
     6121 cctgatccat atcggcgacc gcacggtcgg cggcctttgc tctcgcgacc agctcgtcgg
     6181 cccgtggcag gtccccgtca gcgacgtggc cgtcacggcg tcgggctata acggctacac
     6241 gggcgaggcg ctcgcgatgg gcgagcggac tccggtcgcg atccacgacg gcccggcgtc
     6301 ggcgcggctg gcggtggccg aggcggtgct gaacatcgcg gctgcggacg tggccgcgct
     6361 cggcgacatc cgcttgtccg caaactggat ggcggcggcc ggccacgccg acgacgacta
     6421 cgcgctgtac gcgatggtgc gcgccgtcgg cgaagagctg tgcccggcct tgggcctcgc
     6481 ggtgcccgtc ggcaaggact ccttatctat gcgcaccgtc tggcaggacg gcgggcgcga
     6541 gcgcgccgtc acggcgccgg tctcgttgat agtctcggcg ttcgcgccag tcgtcgacat
     6601 ccgccgcacg ctgacgccgg agctcgaccg cgacgtcgac tcggtgctcg tgttcgtcga
     6661 cttggtcggc ggcgcgcagc ggctcggcgg ctcttctctg gcgcagacgt tcggccaatt
     6721 cggcggcgct gcgccggacg tcgacgacca gcagcgtctc gtgcgttggt ttgccgcgca
     6781 gcgtgcgttg cgcgcggccg atctgttgct cgcctatcac gaccgttccg acggcggctt
     6841 gttcacgacg ctcgtcgaga tggccttcgc ggccaacgtc ggcctcgacg tcgagctcga
     6901 cggtggcgtc gccgacgcga tcgcctactt gttcaacgag gagctcggcg ccgtgatcca
     6961 ggtgccctcc gcgcagctgt cgcgcgtcga gcaggttctc gccgaagcgc aactcgagca
     7021 ccgaacgatc gcgcgcatcg cgcggcatga cgacgtcgtc gtgcggcgtg ccgcgaccga
     7081 gctgtaccgt gcgccgcgcg tcgagctgcg gcgtaaatgg tccgagctga cctataggat
     7141 gcaggcgttg cgcgacgacc cgcagtgcgc gcgcgaggcc ttcgacgccg tgctcgacgc
     7201 gaaggatccg ggtctcaacg ccaagctgac gttcccgctg ccgaacgagc gccgggcggc
     7261 ggcgcgcgcg ggtcggccga aggtcgccgt gctgcgcgag cagggcgtca acgggcagcg
     7321 ggaga
//
LOCUS       DATLEE010000516         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3291030, whole genome shotgun sequence.
ACCESSION   DATLEE010000516 DATLEE010000000
VERSION     DATLEE010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3291030"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1613
                     /gene="gspD"
                     /locus_tag="VM692_11120"
     CDS             <1..1613
                     /gene="gspD"
                     /locus_tag="VM692_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HUQ52763.1"
                     /translation="YVTQAIILDNIGAAQLVPILRPLLPQSAHLAAHQPSNALIVADR
                     PQNMRRMLELIKRMDQAGTQEVEVIALENASADEVVRMLGALNQAAQAAGGAPPIQVI
                     ADARTNSVLLSGSGTVRLQMRALIAHLDTPSAQGGASNVRYLNYADAEDLATKLQAQF
                     GGSGGGGATTPAPAEGGAPPALAGPVSIWADAGTNALVINAPERIREDMDAIINQIDI
                     PRYQVQVDAIIVELSEERSAQLGVTWLRAGEDFLGLTNFSNAGGGGIVGLAGATQGDT
                     PALSALAEGISVGLGKIGDSNSWAALLNALRGDGETNVIATPHIVTLDNEEAEIRVGQ
                     EVPFLTGQFTNTGANQGSVNPFQTIQREEVGTRLKITPQINEGSGVKLEIEQETSSLS
                     ATVAGAADLVTNKRNITTSVFVDDGEVLVLGGLIDDQLRETDRRVPGLGKIPGLGWLF
                     RARKTDRKKTNLMVFIRPSILRDAADARFQTNTKYRYIQDLQRQMADDPVRLMRDVDR
                     PELPPLPEPRPETIPPINIPPRDGSEPR"
     gene            1594..>2403
                     /locus_tag="VM692_11125"
     CDS             1594..>2403
                     /locus_tag="VM692_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HUQ52764.1"
                     /translation="MAASPDSTERVEVPSAAPTQRTLPFAFAKRHGVLIQELRDGAAQ
                     AIYKTGAAAQSIAEVRRFAGVPVRFAEVDPETFDARLQAAYESGAAMTMVEGLNDDTD
                     LLAVAQQLPEPSDLIDSDDEAPIIRLINALLTQAVKDNASDIHIEPYENRMTVRFRID
                     GVLREVLQSKRAVAPLVVSRVKVMSKLDIAEKRLPQDGRISLRIAGRAVDVRVSTIPS
                     GHGERVVMRLLDKQAGRLTLTNLGMSDEDQAVMHELVHRPHGILLVTGPTGS"
BASE COUNT          462 a          808 c          778 g          355 t
ORIGIN      
        1 actacgtcac gcaagcgatc atcctcgaca acataggcgc ggcgcagctc gtgccgatcc
       61 tgcggccgct gttgccgcag tccgcgcatc tggccgcgca ccagccgtcg aacgctctga
      121 tcgtcgccga tcgcccgcag aacatgcggc gcatgctcga gctcatcaag cgcatggatc
      181 aagccggcac ccaagaagtc gaggtgatcg cgctcgagaa tgcttcggcc gacgaggtcg
      241 tgcgtatgct cggcgcattg aaccaagcgg cacaggctgc cggcggcgcg cccccgatcc
      301 aggtcatcgc cgatgcgcga accaacagcg tgttgctgtc gggatcgggc accgtccggc
      361 tgcaaatgcg cgcgctgatt gcgcacctcg acacgccgtc cgcgcaaggc ggcgcatcga
      421 acgttcgata tctcaactac gccgatgccg aggatctcgc aacgaagctg caagcgcagt
      481 tcggcggctc cggcggcggt ggggccacga cgcccgcgcc ggccgagggc ggtgcgccgc
      541 cggcgctcgc gggcccggtg tccatctggg cggatgcggg cacgaacgcg ctcgtcatca
      601 acgcgcccga gcgcatccgc gaggacatgg acgcgatcat caatcagatt gacattccac
      661 gctatcaggt gcaggtcgac gcaatcatcg tagagctcag cgaggagaga tcggcgcagc
      721 tcggcgttac gtggctgcgc gcaggggaag acttcctcgg cctcacgaac ttttcgaacg
      781 ccggcggcgg cggcatcgtc gggctcgcag gtgcgacgca gggcgacacg ccggcccttt
      841 cggcgctcgc cgagggaatt tcggtcgggc tcgggaagat cggcgattcg aacagttggg
      901 ccgcgttgct gaatgccttg cgcggcgacg gcgaaacgaa cgtcatcgcg acgccgcaca
      961 tcgtgacgct cgacaacgaa gaagccgaga tccgtgtcgg ccaagaggtc ccgttcctta
     1021 cgggccagtt cactaacacg ggcgcgaacc agggctcggt gaatccgttt cagaccatcc
     1081 agcgcgaaga agtcggcacg cggctcaaga ttacgcccca aatcaacgaa ggcagcggcg
     1141 tgaagctcga gatcgagcag gagacctcga gcttgagcgc tacggttgcg ggggcggccg
     1201 acctcgtgac gaacaagcgc aacatcacga cctcggtgtt cgtcgacgac ggcgaggtac
     1261 tcgtgctcgg cggcctcatc gacgatcagc tgcgggagac ggatcggcga gtgccggggc
     1321 tcggaaaaat tccgggcctg ggctggctgt ttcgcgcccg caagacggat cgcaagaaga
     1381 ccaacttgat ggtgttcatc cgaccgagca ttctgcgcga cgcggcagac gctcgcttcc
     1441 agacgaatac caagtaccgc tacattcagg atctccagcg ccagatggca gacgatcccg
     1501 tccgcctcat gcgcgacgta gaccggcccg aactgccgcc gctcccggag ccgcggcccg
     1561 aaacgatccc accgatcaac ataccgccgc gcgatggcag cgagccccga tagcaccgaa
     1621 cgggtcgagg tgccgagcgc ggcgccgacc caacggactc tgccgttcgc attcgcgaaa
     1681 cggcatggcg tgctgatcca ggagctgcgc gacggcgcgg cccaagccat ctacaagacc
     1741 ggcgcggccg cccagagcat cgccgaggta cgacgcttcg ccggcgtgcc ggtgcgcttc
     1801 gcggaagtcg atcccgaaac attcgacgcg cggctgcaag cggcctacga aagcggcgcc
     1861 gcgatgacga tggtcgaagg gctcaacgac gacaccgatc tgctcgccgt cgcgcaacag
     1921 ctgcccgagc cgtcggactt gatcgacagc gacgacgaag cgccgatcat ccgcctcatc
     1981 aatgcgctgc tgactcaggc cgtcaaggac aacgcgtcgg acattcacat cgagccgtac
     2041 gagaaccgca tgacggtgcg gttccgcatc gacggagtgc tgcgcgaagt tctgcaatcg
     2101 aaacgcgccg tggcgccgct cgtcgtatca cgcgtcaaag tcatgtcgaa gctcgacatc
     2161 gccgagaagc gcctgccgca agacggccgc atctcgttgc gcatcgctgg gcgcgccgtc
     2221 gatgtccgcg tctcgacgat cccgtcgggg cacggcgagc gcgtcgtcat gcgcttgctc
     2281 gacaagcagg ccggccgcct cacactcacg aaccttggca tgtccgacga ggaccaggcg
     2341 gtcatgcacg agctcgttca ccggccgcac gggatcttgc tcgtgaccgg gcccaccggg
     2401 tcc
//
LOCUS       DATLEE010000517         7255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2828193, whole genome shotgun sequence.
ACCESSION   DATLEE010000517 DATLEE010000000
VERSION     DATLEE010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2828193"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /locus_tag="VM692_11130"
     CDS             complement(<1..1851)
                     /locus_tag="VM692_11130"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HUQ52765.1"
                     /translation="MNAPQQTRRGFIATLGGIVLSFSLDTRHALGQEPARLPGSLQNN
                     RTLDAWLRINADGSVTVFTGKVELGQGIVTALAQIAAEELDVPLARIAMISGDTGRTP
                     NEGQTAGSQSIEQSGTALRMASAEVRALLIELASKKLGVAANALTVTDGVIHAPDGRK
                     VGYGELAAAASLKREATAKVPPKPAAQHRIVGESVPRRDIPAKMTGGAAFVQDLRLPG
                     MVHGRVVRPPRYGARLDGFDEAKVRAMAGVIAVVRDGSFLGVVARREEQAITARLVLS
                     GSAEWSGGSELPDPAKMYVELMSLRSEDRVIGEKDAPVPAGAKVIEATYHRPYQAHAA
                     IAPSCAVAEHKDGKLTVWTHSQGVFPLRATLARALGLEPSDIRCIHAEGAGCYGHNGA
                     DDVAFDAAMLARAVDGRPVRLQWMRDDEFKWEPYGPAMTMKVKGAVAGGRVVDWAYDV
                     WSQTHNMRPGDPDGINLLGSWYLADAKQPGPARHAAQPNGAGDRNALTLYDFPRQRTT
                     HHLIMENPVRTSAMRALGAYANVFAIESFMDELAAAAGVDPVAFRFAHIKDERERAVI
                     EAVARAAAWQPGQQGDGRRGRGIGYARYKTVATYAAVIAEVEVNRTTGIVR"
     gene            complement(1854..2321)
                     /locus_tag="VM692_11135"
     CDS             complement(1854..2321)
                     /locus_tag="VM692_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HUQ52766.1"
                     /translation="MAISLKVNGATHSVDAEPDTPLLYVLRNDLELNGAKYGCAMTQC
                     GACTVLINGAATRSCVTPVGSIEGNEVTTLEGLGTLDKPHALQKAFIDEQAAQCGYCA
                     SGMIMAAADLLNRNPQPQEADVRAALAANLCRCGTHNRIVRAVLRAARQNVGS"
     gene            complement(2334..3044)
                     /locus_tag="VM692_11140"
     CDS             complement(2334..3044)
                     /locus_tag="VM692_11140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUQ52767.1"
                     /translation="MPKRFLAVSIALAVLLVGCQQDSEQAATTPSPASGAAATPPAGT
                     AKLPPLPGKEGAAPAITQVTRVRLATTGGDVVIEVYPEAAPNAAKRFVELVTSGFYDD
                     TPVSRVVPGFVAQFGVNWREPHKSWKTREFVDDPGYFALERGTLAFAKAGQPNTASTQ
                     VFINYRDNNRLTEPSPSLSFWVFGKVVEGMDVVDKFAQVGDPSGGLDQNRLWADGGAY
                     LESLPQKPTMIEKATVEQ"
     gene            3155..4456
                     /locus_tag="VM692_11145"
     CDS             3155..4456
                     /locus_tag="VM692_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ52768.1"
                     /translation="MQATATLSRSDWRLILLASLGGALEFYDFVVYSQFAQYIGRNFF
                     PNTDAMVSLIVSFSVFAVGYFARPLGGIFFSHIGDRIGRRRVLIITILAMSAATFGIG
                     LLPTYAQWGIAASILLVVLRIVQGFCLGGELPGAISYVVETAPRRSGFSVGVVFFCVN
                     TGVALAALLNLVVHETLTVEQIGDWGWRIGFLVGGVLGLVSFVLRLSLEESREFARIR
                     ATARAAAVPMAEVIRTYPAAVVVGVGILAVTAGYNGLLFAMPAFLPQAMGYEPVRAIE
                     AQNAALIIMSFGLLFVAWLTDRVSRKALALLGTALLIVLSWPFFSAAEAKSVSLLALF
                     SLGGIVGSVCAGTVIAISADLFPTRIRFSGVALSYNIAFSVFSGLAPVIAALLARNTG
                     VAATAAYYMIGCAALSFAAALVMHRYDGRIVADLAPETPRG"
     gene            complement(4475..5482)
                     /locus_tag="VM692_11150"
     CDS             complement(4475..5482)
                     /locus_tag="VM692_11150"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52769.1"
                     /translation="MSKHGLAIAVASAVSTPAVWAHHSTALVYDRDGALLDVEGVVTE
                     VAWVNPHVRLKLRGAGPDGVERVWDIESNSVSNVSRFGLTAELVAAGTRVKVAGNGGR
                     QLENVLWLTNMLLPNGKEILFGQAVKPRWSQQTIGSDVRGAVTADTNGLGLYRVWTHA
                     TNPAVFWGGALPLTAGAAAARAAFNPLTDEPTKNCAPKGMPFIMEQPYPMEIVAAADE
                     ILIKMEEYDTVRHVAMKPGVAVRPEAESPLGVSFGRWEGAALVVFTQDIGYRWLNGTG
                     IPLGPDASTEERFDLNADGSRLEYTMKVTDPATFTAPVTFRKAWEWRPGEQVRPYACR
                     G"
     gene            complement(5479..6252)
                     /locus_tag="VM692_11155"
     CDS             complement(5479..6252)
                     /locus_tag="VM692_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUQ52770.1"
                     /translation="MSARSLRAAAALLVALASIGLGSAQPSGQSAPVRALSSNGVRAA
                     LEQVVPQCERTVGRKLDVQFGTSASIKQRIEGGEAIDVAFITSEVVAELAKGGHLAAS
                     SITPLGRAGIGVGIRIGARTYDIGTADAIKQALLTASSVTYAQDGASRVHIESMFEKL
                     GIAQQMKAKTMLEQGSTRAAAKVVDGEAELLLTLVSEILPVADMDLLGPLPKEFQSYI
                     SFDAAVGAHPADLAAARAFVACVASPQGASVFAEKGIER"
     gene            complement(6249..6707)
                     /locus_tag="VM692_11160"
     CDS             complement(6249..6707)
                     /locus_tag="VM692_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUQ52771.1"
                     /translation="MKGLARLFTAALGLATLAVPAISMSDIDRSAVDFTAPADIKWVR
                     NSAGTSEQAVLFGDPSKPGPYVIRIRWLPGNMSRPHFHSTDRFFIVLEGTWYLGTGTK
                     FDPDSTVGVKPGTYVYHKAGGIHYDGAKDEPAVIQVWGTGPMTTTQAEQP"
     gene            complement(6892..>7255)
                     /locus_tag="VM692_11165"
     CDS             complement(6892..>7255)
                     /locus_tag="VM692_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52772.1"
                     /translation="QVGATLQFCAFPYKSAEELSSRWPGVDFSWRSKAATDGSPPRVA
                     GHVMVTADGTKVFWEPHGLLVECVRSSTEPRESWLAFLRSNPRAWCEQRRRPEDWSTV
                     ASRELAAELEAAIGTPCE"
BASE COUNT         1046 a         2496 c         2432 g         1281 t
ORIGIN      
        1 cctgacgatg ccggtggtgc gattcacttc gacctcggct atcaccgccg cgtaggtggc
       61 cacggtcttg tagcgcgcat agccgatgcc gcggccgcgt cggccgtcgc cctgctggcc
      121 cggttgccac gcggcggccc tggccacggc ctcgatcacg gcgcgctcgc gctcgtcctt
      181 gatgtgcgcg aaacggaacg ccacggggtc gacgccggcg gcggccgcga gctcatccat
      241 gaacgactcg atcgcaaaca cgttcgcata ggcgccgagc gcgcgcatcg ccgaggtgcg
      301 caccggattc tccatgatca ggtggtgcgt cgtgcgctgg cgcgggaaat cgtagagcgt
      361 gagagcgttg cggtcgcccg cgccgttcgg ctgtgccgca tggcgcgccg gcccgggttg
      421 cttcgcatcg gcgagatacc aactgccgag caggttgatc ccgtccggat cgccgggccg
      481 catgttgtgg gtctggctcc agacgtcgta ggcccagtcg acgactcggc cgccggcgac
      541 ggcgcccttg accttcatgg tcatggcagg gccgtagggc tcccacttga actcgtcgtc
      601 acgcatccat tgcagccgca ccggccgccc atcgacggcg cgcgcgagca tggcggcgtc
      661 gaaggccacg tcgtcggcgc cgttgtggcc gtagcagccg gcgccttcgg cgtggatgca
      721 gcggatgtcg ctcggctcga gaccgagcgc cctcgcgagg gtcgcgcgca gcgggaagac
      781 gccctggcta tgggtccaga ccgtgagctt gccgtccttg tgctcggcca ccgcgcacga
      841 cggcgcgatg gcggcatgcg cctggtacgg ccgatggtag gtcgcctcga tgaccttggc
      901 gccggcgggc acgggcgcgt ccttctcgcc gatcaccctg tcctcgctgc gcagcgacat
      961 gagctcaacg tacatcttcg ccggatcggg cagctccgag ccgcccgacc actcggcgga
     1021 accgctcagc acgagccgcg ccgtgatcgc ctgctcctcg cgccgcgcga ccacgccgag
     1081 aaagctgccg tcacgcacca cggcgatgac tcccgccatc gcccggactt tggcctcgtc
     1141 gaagccgtcg agcctggcgc catagcgcgg cggccgcacc acgcggccat gcaccatgcc
     1201 gggaagccgc aggtcctgca cgaaggcggc gccgcccgtc atcttggccg ggatgtcgcg
     1261 gcgcggcacg gactcgccga cgatcctgtg ctgtgcggcc ggcttcggcg gcaccttggc
     1321 ggtggcctcc cgcttcaagc tcgctgcggc ggcaagctcg ccgtagccga ccttgcggcc
     1381 gtcaggcgca tggatgacgc catccgtgac cgtgagtgcg ttagcagcga cgccgagctt
     1441 cttcgaggcg agctcgatga gcagtgcgcg cacttcggcg ctcgccatac gtagcgcggt
     1501 gccgctctgc tcgatcgact ggctgccggc ggtctggcct tcattcgggg tccggccggt
     1561 gtcgcctgag atcatcgcga tgcgcgccag cggaacgtcg agctcctcgg cagcgatctg
     1621 ggcgagcgcc gtcacgatcc cctgcccgag ctcgaccttg ccggtgaata cggtgacgct
     1681 cccatcggca ttgatgcgga gccacgcatc aagagtgcgg ttgttctgca ggctgcccgg
     1741 gagccgcgcc ggctcctgcc cgagcgcgtg tctcgtgtcg aggctgaacg agaggacgat
     1801 gccgcccagg gtggcgatga agccgcgacg cgtctgctga ggcgcgttca tcgtcagctc
     1861 cccacgttct gtcgcgcggc gcgcaggacg gcgcggacga tccggttgtg ggtgccgcag
     1921 cggcagagat tggcggcgag cgccgcccgc acgtccgcct cctgcggttg cggattgcgg
     1981 ttcagcaggt cggccgccgc catgatcatg ccgctcgcgc aatagccgca ctgagccgcc
     2041 tgttcatcga tgaaagcctt ctgcagcgcg tgcggcttgt cgagcgtgcc gagcccttcg
     2101 agcgtcgtca cctcgttgcc ctcgatcgaa ccgaccggcg tcacgcagga gcgtgtcgcc
     2161 gcgccgttga tgagcaccgt gcaggcgccg cattgcgtca tggcgcagcc gtacttggcg
     2221 ccattcagct ctagatcgtt gcgcagcaca tagagcagcg gcgtgtccgg ctcggcatcg
     2281 accgagtgcg ttgccccgtt gaccttcagg ctgatggcca ttgtgggtct cctctactgc
     2341 tcgacggtcg ccttctcgat catcgtcggc ttctggggca gcgactcgag atacgcgccg
     2401 ccgtcggccc agagcctgtt ctgatcgagc ccgccgctcg gatcgccgac ctgcgcgaac
     2461 ttgtcgacga cgtccatacc ttcgacgact ttgccgaaga cccagaagct caagctcggc
     2521 gacggctcgg tgagccggtt gttgtcgcgg tagttgatga agacttgcgt cgacgccgtg
     2581 ttcggctgcc ctgccttcgc gaaagccagc gtgccgcgct cgagcgcgaa gtagcccggg
     2641 tcgtcgacga actctcgggt cttccacgac ttgtgcggct cgcgccagtt gacgccgaac
     2701 tgcgcgacga agcccggcac gacacgcgag accggcgtgt cgtcatagaa gccggaggtc
     2761 acgagctcga cgaagcgctt cgccgcgttc ggcgccgctt ctgggtagac ctcgatcacg
     2821 acgtcgccgc ccgtcgtggc gagacgcacg cgcgtgacct gcgttatcgc gggagcggcc
     2881 ccctctttcc cgggcagcgg cggcagcttc gcagtgcccg caggcggagt cgccgccgct
     2941 cccgaagccg gactcggcgt cgtggcggcc tgttcggaat cttgctgaca accgacgagc
     3001 aatacggcca gcgcgatcga gactgctagg aaccgcttcg gcatgacgac ttctccggca
     3061 acgaccaagg gactctaacg cgcgccaatt gcgcgcggca atcgcggcct ggaatactgc
     3121 ggcacgtcga acggccataa ggcgggggag cttcatgcag gcgacggcca cactgtctcg
     3181 ctcggactgg cgcttgatcc tgctcgcgag cttgggcggc gcgctcgagt tctacgattt
     3241 cgtcgtctat agccagttcg cgcaatacat cggccgcaat ttttttccca acaccgacgc
     3301 gatggtgtcg ttgatcgtat cgttcagcgt attcgccgtc ggctacttcg cgcggccgct
     3361 cggcggcatc ttcttcagcc acatcggcga tcgcatcggc cgccgccgag ttttgatcat
     3421 cacgatcctc gcaatgtcgg ccgcgacgtt cggcatcggc ttgctgccga cgtatgcgca
     3481 gtggggcatc gcggcgtcga tcctgctcgt cgtcttacgc atcgtgcagg gcttctgctt
     3541 gggcggggaa ttgcccggcg cgatttcgta cgtcgtcgag acggcgccgc gccgctcggg
     3601 gttctcggtc ggcgtcgtgt tcttctgcgt caatacgggc gtcgcactcg cggcgctgct
     3661 gaatctcgtc gtgcacgaga cgttgaccgt cgagcagatc ggcgactggg gctggcgcat
     3721 cggcttcctc gtcggcggcg tgctcggcct cgtcagcttc gtgctgcgct tgtcgctcga
     3781 ggagagccgc gagttcgcgc gcattcgcgc gacggcgaga gcggccgccg tgccgatggc
     3841 cgaagtgatt cgcacgtatc ccgccgccgt cgtcgtcggc gtcgggatcc tcgccgtgac
     3901 ggccggctac aacggcctgt tgttcgcgat gccggcgttc ctgccgcagg cgatgggcta
     3961 cgagcccgtc cgagccatcg aggcgcagaa cgcggcgctg atcatcatgt cgttcggcct
     4021 gctgttcgtc gcatggctca cggaccgcgt ctcgcgcaaa gcgctggcgc tgctcggcac
     4081 ggcgctgctg atcgtgctgt cgtggccgtt cttcagcgcc gccgaggcga agagcgtcag
     4141 cttgctcgcg ctgttctcgc tcggcggcat cgtgggctcc gtctgcgccg gcacggtcat
     4201 cgcgatctcg gccgatttgt tcccgacgcg catccgcttc tcgggtgtgg cgctgtcgta
     4261 caacatcgcg ttctcggtct tcagcgggct ggcgcccgtc atcgcggcgc tgctcgcgcg
     4321 caataccggc gtcgctgcga cggcggcgta ctacatgatc gggtgcgccg ccctatcgtt
     4381 tgccgcggcg ctggtgatgc accgctacga cgggcgcatc gtggcggacc ttgcgcccga
     4441 gacgcctcga ggctaacgct ctaggtctcg gcgctcagcc gcggcacgcg tatgggcgta
     4501 cttgctcgcc ggggcgccat tcccaggctt tgcggaacgt gacgggggcg gtgaaggtcg
     4561 cgggatcggt gaccttcatc gtgtactcga ggcggctgcc gtcggcgttg aggtcgaagc
     4621 gctcttcggt cgacgcgtcg gggccgagcg ggataccggt gccgttcagc cagcgatagc
     4681 cgatgtcttg cgtgaagacg acgagcgccg cgccttccca gcggccgaac gacactccga
     4741 gcggtgattc cgcttcgggc cgcactgcga cgccgggctt catggcgaca tgccgcacgg
     4801 tgtcgtactc ctccatcttg atgaggatct cgtcggccgc cgcgacgatc tccatgggat
     4861 acggctgctc catgatgaac ggcatgcctt tcggcgcgca gttcttcgtc ggctcgtcgg
     4921 tcagcggatt gaaggcggcc cgagccgccg cggcgcccgc ggtcagcggc agggcgccgc
     4981 cccagaacac ggccggattc gttgcatggg tccagacgcg gtacagcccg aggccgtttg
     5041 tgtccgccgt gacggcgccg cgcacgtcgc tgccgatcgt ctgctgcgac cagcgcggct
     5101 tgacggcctg cccgaacaag atctccttgc cgttcggcag cagcatgttc gtgagccaca
     5161 ggacgttctc gagttggcgg ccgccgttgc cggcgacttt gacgcgtgtg ccggcggcca
     5221 cgagctcggc cgtcaaaccg aagcggctca cgttactgac ggagttcgac tcgatgtccc
     5281 agacgcgctc gacaccgtcg ggccccgcgc cgcgaagctt caagcgcacg tgcggattga
     5341 cccacgccac ttccgtaacg acgccctcga cgtcgagcaa cgcgccatcg cggtcataga
     5401 cgagcgccgt cgaatgatgt gcccacacgg ccggcgtcga cacggccgac gcgacggcga
     5461 tcgctagccc gtgcttgctc atcgctcgat gcccttctcc gcgaacaccg acgctccctg
     5521 aggcgacgca acgcacgcga cgaacgcacg cgctgcggca aggtcggccg ggtgcgcgcc
     5581 gactgcggcg tcgaacgaaa tgtagctttg aaactccttc ggcaacgggc cgagcaggtc
     5641 catgtcggcc acgggcaaga tctcgctgac gagcgtgagc agcagctccg cctcgccgtc
     5701 gacgaccttg gcggccgcac gcgtcgagcc ctgctccagc atcgtcttcg ctttcatttg
     5761 ctgcgcgatg ccgagcttct cgaacatgct ctcgatgtgc acgcggctcg cgccgtcctg
     5821 cgcatacgtg accgagctcg ccgtcaacaa cgcctgcttt atcgcatccg ccgtgccgat
     5881 gtcgtaggtg cgagcgccga tgcgaatacc gacgccgatg ccggcgcgcc ccagcggcgt
     5941 gatcgagctc gcggccaagt gcccgccctt cgcgagctcg gcgacgacct cgctcgtgat
     6001 gaacgcgacg tcgatggctt caccgccctc gatgcgttgc ttgatcgatg ccgaggtgcc
     6061 gaactgtacg tcgagcttgc gcccgaccgt gcgttcgcat tgcggcacca cttgctcgag
     6121 cgccgcgcgc acgccgttcg acgacagcgc gcggaccggc gcgctctgcc ccgaaggttg
     6181 cgcggacccg agtccgatcg atgcaagcgc aacgagcaac gcggccgcgg cgcgcaggct
     6241 tcgcgcgctc atggctgttc ggcctgcgtc gtcgtcatgg ggcccgtgcc ccacacttgg
     6301 atcacggccg gctcgtcctt cgcgccgtcg taatgaatgc cgccggcctt gtgataaacg
     6361 tacgtgccgg gcttcactcc gacggtgctg tccggatcga acttggtgcc ggtgccgaga
     6421 taccacgtgc cctcgagcac gatgaaaaac cgatccgtcg agtggaagtg cggccggctc
     6481 atgttgccgg gcagccagcg gatccgaatc acgtaagggc ccggcttcga cggatcgccg
     6541 aacagcacgg cttgctcgct cgtgccggcg gagttgcgta cccacttgat gtcggcgggc
     6601 gccgtgaagt cgacggcgct gcggtcgatg tcgctcatcg agatcgccgg cacggccagc
     6661 gtcgctaacc cgagagccgc cgtgaagagg cgagcgagac ctttcatggt gcgtccttct
     6721 tcgagttggg atgcaccgat ggtaggccca aatacgtgaa ggcgcgagcc tggcgcgcgc
     6781 tactgcgtcg agttgcgcgg caagcctgcg aggccagaaa taggccgaca atccctcgca
     6841 gctaagcgct gcagtgaacg ctacgttgcg ggctcggcta cgcggcccgg gctactcgca
     6901 aggagtgccg atggcggcct cgagctccgc tgccagctcg cgcgacgcca cggtcgacca
     6961 gtcctccgga cgccgccgct gctcgcacca ggcccgcggg ttcgacctca agaaagctag
     7021 ccatgactct cgcggctccg tcgagctgcg gacgcattcg acgaggagcc cgtgcggttc
     7081 ccagaacacc ttggtgccgt cggccgtgac catgacgtgc ccggcaacgc gcggcggcga
     7141 gccgtccgtg gccgccttcg atcgccacga aaaatcgacg ccgggccatc ggctcgacag
     7201 ctcttccgcg gatttgtacg gaaaggcaca gaactgcagc gtggcgccga cttgc
//
LOCUS       DATLEE010000518         3374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2085100, whole genome shotgun sequence.
ACCESSION   DATLEE010000518 DATLEE010000000
VERSION     DATLEE010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2085100"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VM692_11170"
     CDS             <1..632
                     /locus_tag="VM692_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52773.1"
                     /translation="VVVFAALSIVLLTATVYTYDRLTAETLIAELRFDATGERQYVAH
                     LRTGDRCAERTFPVLGDQWRVDAEFLKWKYWALLLGLDSQYRLDRLEGRYRSVADQNS
                     QANIAHDLSERTAVDLVALAESLGSWNFLLDATYGSSTYQDIDVANVYYVYRTTTGII
                     TRYEPRPVQPVGEPLNVKIDGACGEPSMWRRFTAWTDDGVVSALDLVTP"
     gene            657..1238
                     /locus_tag="VM692_11175"
     CDS             657..1238
                     /locus_tag="VM692_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ52774.1"
                     /translation="MGMRIRSLIAVALAVAIGFVMARVGDGGSAAAQPSALSALDYQE
                     ITQLINRYAYGIDTCADNGYDYADVFTTDGVFIDRNSDAGFAAGGRVLAQGREALATL
                     VGGGSRGCATKLVWTDWSHLMLNHEITPSAEGATGRVYLVQLGMDGPGSVARHGGYED
                     VYVRTPEGWRIKSRTHVRNKAWHSEKLRTPDLN"
     gene            complement(1235..1777)
                     /locus_tag="VM692_11180"
     CDS             complement(1235..1777)
                     /locus_tag="VM692_11180"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HUQ52775.1"
                     /translation="MPTYVALLRAVNVAGHARVPTAKLVQLLAYAGALDVASFGHAGN
                     LRFTARSGAVGIVARFRDALERRHGERPVVVLRTAADFAALTSAQPFADCGAAQSDKL
                     YVTFLARKPRVQPSLPLVSAAERLTVLECRGRDVLLKSVRKPNGFYGLPNAFVETAFG
                     VPATTRNWSTVTRLAKLLAS"
     gene            1828..2334
                     /locus_tag="VM692_11185"
     CDS             1828..2334
                     /locus_tag="VM692_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HUQ52776.1"
                     /translation="MEPVTIVLWIAASVLIVIGAVGLVLPVLPGAPLIFIGALLAAWA
                     EEFRYLGMWSLIVLGALAVLAVVVDFVASAFGVKRFGASGRAVTGATLGAIVGLFFGG
                     PVGVLVGPFIGAVVGELTVRRSLAAAGRAGVGAALGLALGTAGKLAIAFAMIGLIVFM
                     RLLSAPTV"
     gene            2432..2857
                     /locus_tag="VM692_11190"
     CDS             2432..2857
                     /locus_tag="VM692_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HUQ52777.1"
                     /translation="MVIRVRDPFAALLGLQRAMDSVMGSDWFGSRTSGTGAFPLINVF
                     NDGEDFVLVAELPGVKKEDLDVQVRGDTLRIQGKKTVAYDESASAHRRERAAGQFDRT
                     LTLPAEVDAAKVAAEYRDGVLTLRLPRAESAKPRTVTIN"
     gene            2888..3283
                     /locus_tag="VM692_11195"
     CDS             2888..3283
                     /locus_tag="VM692_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HUQ52778.1"
                     /translation="MVTQEVAPREKREVAPAEERTEAGKFFSPYTDIHETERSVVVAM
                     EMPGVDKSSIDIRLDKGVLTVKGNVDGSKYESWRPIYSEYNVGNFVRTFTVSPKIDSA
                     AISATVADGVLTIELPKAAEAVAKRISVR"
BASE COUNT          565 a         1083 c         1153 g          573 t
ORIGIN      
        1 ccgtcgtggt gtttgcggct ctgtcgatcg tgctgctgac ggcgacggtc tacacctacg
       61 acaggctcac ggccgagacg ctgatcgccg agctccgctt cgacgcaacc ggcgagcggc
      121 agtacgtcgc gcacttgagg accggcgatc gctgcgccga gcgcacgttc cccgtgctcg
      181 gcgatcaatg gcgcgtcgac gcggagttct tgaagtggaa gtactgggcc ctgctgctcg
      241 gtctcgattc gcagtatcgg ctcgatcgcc tcgaagggcg ctaccgctcg gtcgcggacc
      301 agaactcgca ggcgaacatc gcacacgatc tcagcgagcg cacggccgtc gatctcgtcg
      361 cgttggcaga gtcgctcggc tcgtggaatt tcctgctcga tgcgacgtac ggcagctcga
      421 cgtatcaaga catcgacgtt gccaacgtct actacgtgta tcgcacgacg accggcatca
      481 tcacgcgcta cgagccgcgg cccgtgcagc cagtaggaga gccgttgaac gtcaaaatcg
      541 acggcgcatg cggcgagccg tcgatgtggc gccgcttcac ggcctggacc gatgacggcg
      601 tcgtgtcggc cctcgatctc gtgacaccgt agaacaagcg cactggggag aacggcatgg
      661 gtatgcgtat tcgctcgctg atcgccgtgg cattggccgt cgcgatcggc ttcgtcatgg
      721 cgcgagtcgg cgacggcggc agcgccgccg cacaaccgag tgccttgtcg gcgctcgatt
      781 accaggaaat cacacagctc atcaatcgct acgcctacgg catcgacacg tgcgccgaca
      841 acggctacga ctacgccgac gtgttcacga ccgacggcgt cttcatcgac cgcaactcgg
      901 acgccggctt cgctgcgggc ggtcgcgtgc ttgcacaggg gcgcgaggcg ctcgcgacgc
      961 tcgtcggtgg cggcagccgc ggctgcgcga cgaagctcgt ctggacggac tggagccact
     1021 tgatgctgaa ccacgagatc acgccgtcgg ccgaaggcgc gacgggtcgc gtctatctcg
     1081 tgcagctcgg catggacggc cccggcagcg tcgcgcggca tggcggctac gaggacgtct
     1141 acgtgcggac gcccgaaggc tggcgcatca agtcgcgcac gcacgtgcgc aacaaagctt
     1201 ggcacagcga gaagctgcgg acgcccgatc tgaactaaga cgcgagcagc ttcgcgaggc
     1261 gcgtgacggt cgaccagttg cgcgtcgtgg cgggcacgcc gaacgcggtt tcgacgaacg
     1321 cgttcggcaa gccgtagaag ccgttcggct tgcgcacgct cttcagtagc acgtcgcggc
     1381 cgcggcactc gagcaccgtg agccgctcgg ccgccgacac gagcggtagg ctcggctgta
     1441 ctcgcggttt gcgcgccagg aacgtcacgt agagcttgtc gctttgcgct gcgccgcagt
     1501 cggcgaacgg ttgagcgctc gtcagcgcgg caaagtcggc ggcggttcgc agcacgacga
     1561 cgggacgctc gccgtgccgt cgctcgagcg cgtcgcggaa gcgcgcgacg atgccgactg
     1621 caccgctgcg cgcggtgaac cggagattgc cggcgtggcc gaacgacgca acatcgagcg
     1681 ctccggcgta cgcgagcagt tgcacgagct ttgcggtcgg cacgcgcgcg tggcccgcta
     1741 cgttcacggc ccgcaataac gcgacgtacg tcggcatgat cggctagatt agagccgccg
     1801 ccgaaaggcc gtcaaagacg cccgccgttg gaaccggtca cgatcgtgct ttggatcgca
     1861 gcgtccgtgc tgatcgtcat cggcgcagtc gggctcgtgt tgcccgtgtt gccgggcgcg
     1921 ccgctgatct tcatcggcgc gctgctcgcc gcctgggcgg aggagttccg gtatctcggc
     1981 atgtggtcgc tcatcgtcct cggcgctttg gcggtgctcg ccgtcgtcgt cgacttcgta
     2041 gcgagcgcgt tcggcgtcaa acgcttcgga gcctcgggcc gcgccgtcac gggcgccacg
     2101 ctcggcgcca tcgtcgggct gttcttcggc ggccccgtcg gcgtgctcgt cgggccgttc
     2161 atcggcgcgg tcgtcggcga gctgacggta cgccgcagct tagcggccgc ggggcgcgcc
     2221 ggtgtcggcg cggcgctcgg gctcgcgctc ggcacggcgg gcaagctcgc catcgcgttc
     2281 gcgatgatcg gcctgatcgt cttcatgcgc ctcctcagcg ctcccacggt ctaacccgta
     2341 gtttccgtaa tcttgcgccg gccttgaatc gccgcggcga cgtccccacc cagtgggcca
     2401 gtggtagttc actgaagcag gaggccatcc gatggtcatt cgagtgcggg atccgttcgc
     2461 tgctttgctc ggcttgcagc gggcgatgga cagcgtcatg gggagcgatt ggttcggtag
     2521 ccgcacgtcg ggtaccgggg cgtttccgct gatcaacgtc ttcaacgacg gcgaggactt
     2581 cgtgctcgtt gcggagttgc cgggcgtcaa gaaggaagac ctcgacgtcc aagtgcgcgg
     2641 cgacacgttg cgcatccaag gcaagaagac ggtcgcctac gacgagagcg cgagcgcaca
     2701 ccgccgcgag cgtgcggccg ggcagttcga tcgcaccttg acattaccgg ccgaggtcga
     2761 cgccgcgaag gtcgccgcgg agtaccgcga cggcgtgttg acgctgcgct tgccgcgcgc
     2821 cgagagcgct aagccgcgca ccgtcacgat caattaaacg acgattgaac gatagaggtg
     2881 atgccaaatg gttacgcagg aagtagcacc gcgcgaaaaa cgcgaggtcg cgccggccga
     2941 agagcgcacg gaagccggca agttcttcag cccgtacacg gacatccatg agacggagcg
     3001 atccgtcgtc gtcgcgatgg agatgccggg cgtagacaaa agctcgatcg acattcgcct
     3061 cgacaaaggc gtgctgacgg tcaagggcaa cgtcgacggg tcgaagtacg agtcgtggcg
     3121 tccgatctac tccgagtaca acgtcgggaa tttcgtgcgc acgtttaccg tgtcgccgaa
     3181 gatcgatagc gccgcgattt ccgcgaccgt cgccgacggc gtgctgacga tcgagttgcc
     3241 gaaggccgcg gaggccgtcg ccaaacgtat ctcggtccgc tagcgagtga tgatggtgcc
     3301 ggtgtttcgg tctcggttgg tacgggcggt ctatcggccg acaggttaac gaaccgaaac
     3361 cgaaacaccg gcac
//
LOCUS       DATLEE010000519         4672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2832545, whole genome shotgun sequence.
ACCESSION   DATLEE010000519 DATLEE010000000
VERSION     DATLEE010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2832545"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VM692_11200"
     CDS             <1..434
                     /locus_tag="VM692_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HUQ52779.1"
                     /translation="KSMLMGVVAGVGVATAGGVLGYQFLGKPGAEVAVEEQAPAHAAP
                     ATRTAAAPAAPKEECWDEQVAVTAGPKDEHRIAGTAIGAVVGGAVGKDVGDRDITTAV
                     GAAAGALIGRKIQERVQENNADKRTTTTVERRCGPPGSAPR"
     gene            580..1758
                     /locus_tag="VM692_11205"
     CDS             580..1758
                     /locus_tag="VM692_11205"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HUQ52780.1"
                     /translation="MIRSTIFWIHLACGVVAGLVVLMMSVTGVLLTYERQIVAWAERS
                     TYPAPAPGAERLTLEELVAAAKLRRPEFTATTVMLRNEPDAPVVLGAGRSGSLLVDPY
                     SGDVREPGPQALRSFFAAVTGWHRWFNATGESRATARAITGGSNLAFLFLVLSGIYLW
                     LPRMWKWALFKTRLFFNPKAGSAKARDFNWHHVFGIWSAIPLAVVVATAAVFSYPWAN
                     DLVYRIAGEEPPRQGGGGPPRGAGGLAEAAVAPSGTAIDRLGYDALLARAAAHGDWRT
                     LTLNIPANPDASSVRVGIDEGNGGQPYLRHMLTLDASTGAVESWAPFSSQTTGQKARS
                     WIRFLHTGEALGVVGQTIAGLVSLTSVLMVWTGLALAWRRLVQPLLRRKAVTSTPEAA
                     "
     gene            complement(1733..2176)
                     /locus_tag="VM692_11210"
     CDS             complement(1733..2176)
                     /locus_tag="VM692_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52781.1"
                     /translation="MTRTTRKPGSRTPRIDTPIDAFDDEGVRLAILAALEAVYECFPH
                     ITLPQYLITLEVRKAERDGHPHTLASIVKKLRMPFSTASRVVWSLTEEGGDIGIIRYL
                     NHPTDRRKKLLVINERNEPNEIPRAISRAMLDYYGEAVKQLRGST"
     gene            2343..2525
                     /locus_tag="VM692_11215"
     CDS             2343..2525
                     /locus_tag="VM692_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52782.1"
                     /translation="MAAKKTRRDEREVPALPFDVTQEIDPSLAEQLRRTSEPTLTQVD
                     FEDITVVLPPKPKPRP"
     gene            2623..3882
                     /locus_tag="VM692_11220"
     CDS             2623..3882
                     /locus_tag="VM692_11220"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUQ52783.1"
                     /translation="MSTFPMRPHLTRPSFLRVVVVLLALAAAPWAEAQNRLASSAEID
                     SYLTSVVRDTRIPGIVALVVDADRILYTGAFGRQDVAGGVPMANDTIFRIASMTKPVT
                     SVAVMMLVQEGDIGLDDPVSDYLPAFENQRVIENFDPADKSYTTRPAATPMTIRHLLT
                     HTSGLGYAFSNHTLAALVGADPGASVTSLPLLHDPGTRWTYGESTRVLGTLVEEVSGQ
                     PLDEFMSERIFVPLGMADTFYTVPPQKARRVVTVHRTTADGLVETPNTEPITAPVYGD
                     GGLHSTAADYAKFIQLFLNNGRAPNGMRLLSEKTVALMGENHIGAVVVEQQPGALPAL
                     SEPFPLGAGRDKFGLGFQVTGAHSEPFTRSAGSMSWAGIFNTEFWIDPARGVGGVLLM
                     QYLPFYDGAAIATLQGFEHRVYEGLAD"
     gene            3863..>4672
                     /locus_tag="VM692_11225"
     CDS             3863..>4672
                     /locus_tag="VM692_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUQ52784.1"
                     /translation="MKGSPISEPSASTSSPLAGAAFGALAALIWAGFPAITKLSMASN
                     ALDAWDVTALRFGVAGLLLLPLFARRRLGTLGSLRAFFLACGAGAPYVLLTAGGLAFA
                     PGGHMGVITPSCMLLCSTLGSRLILKEPLTGSRIAGALAIMAGLVALGWDGLSNHGDL
                     TWLGDSMFALGGLLWASYTIGARVWRVEPLHATAIVGVLSMLLYIPIYAWLGGAHLAS
                     APWREIVIQAVFQGVLSAIVALLLYTRAVAILGAARGAVFAALVPTFSLLLA"
BASE COUNT          739 a         1556 c         1524 g          853 t
ORIGIN      
        1 acaagtcgat gttgatgggt gtcgtagcgg gcgtcggcgt cgccaccgcg ggtggcgtgc
       61 tcggctacca gttcctcggc aaaccgggcg cagaagtcgc cgtcgaagaa caggccccgg
      121 cccacgcggc gccagcgact cgcacggcgg cagcgccggc ggcccccaag gaggagtgtt
      181 gggacgagca agtcgccgtg acggccggcc cgaaagacga gcatcgcatc gcgggcaccg
      241 cgatcggcgc cgtcgtcggc ggtgcggtcg gtaaagacgt cggcgatcgc gacatcacga
      301 cggcggtggg tgctgccgcc ggcgctctga tcggccgcaa gatccaggag cgcgtccagg
      361 agaacaacgc ggataaacgc acgacgacga ccgtcgagcg ccgctgcggc ccgccgggta
      421 gcgccccccg ctaacccgcg gatcggagtc gggaggccgg accggcaacg gtccggcctc
      481 ttttttttgg cgcccacgcc aaccgttctt aaatgcgaac gatttgcgac accattcgtg
      541 tttgggtagg ctggaggtcc actcacaggc tgggcctcga tgattcgctc gacgatcttt
      601 tggattcacc tcgcctgcgg cgtcgtcgcg ggcctcgtcg tcctcatgat gtcggtcacg
      661 ggcgtcctgt tgacctatga gcggcagatc gtcgcctggg ccgaacgctc gacgtacccc
      721 gctcccgcgc cgggcgccga gcgcctgaca ctcgaggagc tcgtcgcggc cgccaagctg
      781 cgccggcccg agttcactgc aacgaccgtc atgctgcgca acgagcctga tgcccccgtg
      841 gtgctgggcg ccggccgcag cggttcgctg ctcgtcgatc cttattcggg cgacgtccgg
      901 gagcccggac cgcaagcgtt gcggagcttc ttcgccgccg tcacggggtg gcatcgctgg
      961 ttcaatgcaa ccggtgagag ccgcgccacg gcacgcgcga tcacgggcgg cagcaacctc
     1021 gcgttcctgt tcctcgtgct gagcggcatc tacctctggc tgccgcgcat gtggaagtgg
     1081 gcgctattca agacgcgtct gttcttcaac ccgaaggccg gctcggccaa ggcccgcgac
     1141 ttcaattggc atcacgtgtt cgggatctgg tcggcgattc cgctcgcagt cgtcgtcgcg
     1201 accgcggcgg tcttctcgta tccgtgggcc aacgacctcg tttatcgcat cgccggcgaa
     1261 gagccgcctc gccaaggcgg cggcgggccg ccgcgcgggg ccggtggcct agccgaagcc
     1321 gccgtcgcgc cgagcggcac ggccatcgat cgcttgggct acgacgcgtt gctcgcgcgc
     1381 gccgccgcgc acggtgactg gcggacgttg acgctgaaca ttcccgccaa cccagacgcg
     1441 tcctcggtgc gcgtcggcat cgacgagggg aacggcggtc agccgtatct gcgccacatg
     1501 ctgacgctcg atgcgtcgac gggcgcagtc gaaagctggg cgccgttctc gagtcagacg
     1561 acgggccaga aggcgcgctc gtggatccgt ttcctgcaca cgggcgaggc gctcggagtc
     1621 gtcggccaaa cgattgccgg gctcgtgtcg ctcacgagcg tgctgatggt gtggacaggg
     1681 ctcgcgctcg cctggcggcg gctcgttcag ccgctgctgc gccgcaaagc cgttacgtcg
     1741 acccccgaag ctgcttaacg gcttctccgt agtagtccag catcgcgcgc gagatggcgc
     1801 gcgggatctc gttcggctcg ttgcgctcgt tgatgacgag caatttcttg cgccggtcag
     1861 tcggatggtt caggtagcga atgatgccga tgtcgccgcc ttcttccgtc agcgaccaca
     1921 ccactcgcga cgccgtcgag aacggcattc tgagcttctt gacgatcgaa gcgagcgtat
     1981 gagggtgccc gtcacgctcg gccttccgaa cctcgagcgt gatcaagtac tgcggcaacg
     2041 tgatgtgtgg aaaacattcg tagacggctt cgagtgccgc gagaatcgca aggcgcacgc
     2101 cttcgtcgtc gaatgcatcg atcggcgtat cgatccgagg ggtgcgggac cctggctttc
     2161 ttgttgttcg tgtcatcagg gtttctccac gttcgaatct caaaaccatg ctagctctcc
     2221 agccgatcct gtcaatcgga actacccact ttgtgcaggt gacacggtta cgtgacacgg
     2281 ttaacgtcgt gaacatgtgc gcgaaatgta ggcttccatc ggtgcaacag agagaccgct
     2341 gaatggccgc caaaaaaact cggcgtgacg agcgcgaagt tccggctctt ccattcgacg
     2401 taacgcaaga aatcgacccc tcgctggccg aacagctgcg acgtacgagc gagccgacgc
     2461 tgactcaagt cgacttcgag gacatcacgg tcgtcttgcc gccgaagccg aagccgcgac
     2521 cctgaacgcg gctgcgtgct agccgccgac ggagcgctct tgcgcgctcg acggccgatt
     2581 cgctagtgtt gcgcaccgac gagccaaccc ggccgtcggt tcgtgagcac gttcccaatg
     2641 cgcccccatc tgacccgacc ttcgttcctc cgcgtcgtcg tcgtcttgct ggcgctggcc
     2701 gcagcacctt gggccgaggc gcagaaccga ctcgcgagca gtgccgagat cgattcttat
     2761 ctaacgagcg tcgtccgtga cacgcgaatt ccgggcatcg tcgcgctcgt cgtcgacgcc
     2821 gaccgcatcc tttatacagg cgcgttcggg cggcaagacg tagcgggcgg cgtgccgatg
     2881 gcaaacgaca cgatcttccg catcgcctcg atgacgaaac ccgtgacgtc cgtcgccgtc
     2941 atgatgctcg ttcaggaagg cgacatcggc ctcgacgatc ccgtctccga ctacttgccg
     3001 gcgttcgaga atcaacgtgt catcgaaaac ttcgatccgg ccgataaaag ctacacgact
     3061 cggcccgccg cgactccgat gacgattcgt catctgctga cgcacacgtc gggattgggc
     3121 tacgcgttct cgaatcacac gctcgccgcg ctcgtcggcg ccgatccagg cgccagtgtc
     3181 acgagcttgc cgctgctgca cgaccccggc acgcgctgga cctacggcga gagcacgcgc
     3241 gtacttggca cattagtgga agaagtgtcg ggccaaccgc tcgacgaatt catgtcggag
     3301 cgcatcttcg ttccgctcgg catggcagac accttctata cggtgccgcc gcaaaaggcc
     3361 cgccgtgtcg tgacggtcca tcgcacgacg gccgacggtc tcgtcgaaac gccgaacacc
     3421 gagccgatca cggcgcccgt gtacggcgat ggcggcctgc actccacggc cgccgactac
     3481 gcgaagttca ttcaactatt cctgaacaat ggccgcgcac cgaacggcat gcggctcttg
     3541 agcgaaaaga ccgtcgcgct catgggcgag aatcacatcg gcgccgtggt cgtcgagcag
     3601 cagccgggtg cactgcccgc gctcagcgag ccgttcccgc tcggcgccgg ccgcgacaag
     3661 ttcggcctgg gatttcaagt cacgggagcg cacagcgagc cgttcacgcg ttcggcgggc
     3721 agcatgagct gggccggcat cttcaacacg gagttttgga tcgatcccgc gcgcggtgtc
     3781 ggcggcgtgc tgctgatgca gtacttgccg ttctacgacg gagccgccat cgcaacgctg
     3841 cagggcttcg aacaccgcgt ctatgaaggg ctcgccgatt agcgagccat cggcctcgac
     3901 ctcctcgccg ctcgcgggcg ccgcgttcgg cgcattggcc gcgctgatct gggcgggatt
     3961 tccggccatc acgaaattgt ccatggcgag caacgcgctc gacgcatggg acgtgaccgc
     4021 gctgcgcttc ggggtcgcgg gcttgttgct gttgccgctg ttcgcacgac gccggctcgg
     4081 aactctgggc tctttgcgcg cgttcttcct cgcgtgcggc gcaggagctc cgtacgtgct
     4141 gttgaccgcc ggcggtctcg cgttcgcgcc cggcggccac atgggcgtca tcacgccgag
     4201 ctgcatgctg ctgtgctcga cgctcggtag ccgcctgatc ctcaaggagc cgttgacggg
     4261 cagccgcatc gcgggcgcgc tcgcgatcat ggccggcctc gtcgcgctcg gttgggacgg
     4321 cctctcgaat cacggcgatc tcacgtggct cggcgattcg atgtttgcgc tcggcggctt
     4381 gctatgggcg agctacacga tcggcgcgcg cgtgtggcga gtcgagccgc tacacgcgac
     4441 ggcgatcgtc ggcgtgttgt cgatgctgtt gtacattccg atctatgcgt ggctcggcgg
     4501 cgcgcatctc gcctcggcgc cttggcgcga gatcgtcatt caggccgtct tccagggcgt
     4561 gctgtcggcc atcgtcgcgc tgctgctcta cacgcgagcc gtcgcaatct tgggggcggc
     4621 gcgcggcgcc gtgttcgccg cgctcgtgcc gacgttctcg ctgctgctcg ca
//
LOCUS       DATLEE010000520         3280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7747370, whole genome shotgun sequence.
ACCESSION   DATLEE010000520 DATLEE010000000
VERSION     DATLEE010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7747370"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..758)
                     /locus_tag="VM692_11230"
     CDS             complement(177..758)
                     /locus_tag="VM692_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HUQ52785.1"
                     /translation="MRNTAKTTVLMMSAFVAWAAPVASVLADPPAHAPAHGWRRKHDP
                     YYVGYQGQQWERDFDISSGSCNRQEIATVIGGVAGGVIANRVADEHKPLATIVGAIAG
                     AVIGNRVGRALDEADRGCFGHALEIGMAGQRITWTNDKSGLRYELSPGADRPSNGKAC
                     REYTLVTAVGRNDRSSHTGLACRSAVGVWQVAQ"
     gene            1049..1573
                     /locus_tag="VM692_11235"
     CDS             1049..1573
                     /locus_tag="VM692_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52786.1"
                     /translation="MTTTNVFRFGGILCAVCAVGAARAEEPASAAEMRSLDEQVQEIK
                     TDVLGIAAELSRLEEKLLYPSGTQIAIFVSIAEGETFRLDSMQIDIDGALATHYLYGF
                     KELDALQHGGVQRVYTGNVPTGAHAIAVSINGKTNGGDEFNSTKEFSFDKGVEPKLLG
                     IALNGPAAIELGEW"
     gene            1608..>3280
                     /locus_tag="VM692_11240"
     CDS             1608..>3280
                     /locus_tag="VM692_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ52787.1"
                     /translation="MPMRVNSLLGLFACGFAAVAVAGPPPFGDFQGSRPFGDFQGSPP
                     DDEEAASRARDWYFGEALYYAQQGHYFEALERLDAELGQHRAVDEPALDSLYRDLREA
                     EFSVGDFELNYRMHHRAGRAITAVLEGAVDEEVRNDAAYRLARIHFQKGQLEDALQAL
                     DRITGRVPEGIVDEIEFLRANVYTGLGRHAEAAEVLRRLQNADGLKGFAAYNLGIALL
                     EDEHRQDALAQLDRAGQIASGDEPVLAIRDKSNFVLGTLLVEAQDFDSAKRYFDRVRL
                     DGPFSNAALLASGWAATAANDYERAVVPWSLLAQREVTDAAVQEALIALPYAYGKLDV
                     HGRAALLYAHALESFGHEIQRLDDSLTSIRDGKFLKVLVREEVRQDKDWVIRLRSLPE
                     SPETYYLMELLASHDFQTALQNYLDLEDLRRKLASWDVSFDSFDDLVGLRRGYYEPQL
                     PGVDADFRELDSRMRLRLEQHELLAKRLQAQLTAPRPELLATGEERMTATRLDELAAA
                     LEDDTSPTADALRQRIARLRGTLSFTLRTEYHERLDVFAKHLRDLGKAI"
BASE COUNT          557 a         1100 c         1092 g          531 t
ORIGIN      
        1 tgggccaaag ctctgcgcag gtcccgccaa gggcacggct ctaatggatc actacaaagc
       61 ttcgaaggca ctcccggccc acgccggcga tacaagcgct aacaacaaag gccgggccgg
      121 cggcgagagg cgcgacctat ccgtcgcccg gccgccacca ctcagcgcaa acggtcttac
      181 tgcgcgactt gccagacacc gactgcggag cggcacgcga gacccgtgtg cgagctgcgg
      241 tcgttgcgcc cgacggccgt cactagcgtg tactcgcggc aagccttgcc gttgctcggg
      301 cgatcggcgc ccggcgacag ctcgtagcgc agcccgctct tgtcgttcgt ccacgtgatg
      361 cgctggccgg ccatgccgat ctcgagcgcg tggccgaagc agccgcggtc agcctcgtcg
      421 agcgcgcggc cgacgcggtt gccgatcacg gcgccggcga tcgcgcccac gatcgtcgcg
      481 agcggtttgt gctcgtcggc gacgcgattc gcgatgacgc cgccggcgac gccgccgatc
      541 acggtcgcga tctcttggcg gttgcagctg cccgacgaga tgtcgaagtc gcgctcccac
      601 tgctgacctt ggtagccgac gtagtacgga tcgtgcttgc ggcgccagcc gtgggccggc
      661 gcgtgcgccg gcggatcggc gagcacgctc gcgaccggag ccgcccaggc gacgaacgcc
      721 gacatcatca atacggtggt cttcgcagtg ttgcgcattc gaaatgtcct ccccgaattc
      781 ggccattgtc cgatcgcagc gtgcagcttg tgtgcgaccg cgatcacaga ctcgcggccg
      841 agctggccgc cgttcgctat atatagaagg agtcgtgcgc agagcagcgc agcaagattc
      901 acgcagattt cgtgcgccgc gtccacgtgt ctccaaaggc cgacgtcacc gttttgcgtc
      961 agtaattgtg ggctggttca cacaagcgtc aggttaattt cacggacact tgcgccgcat
     1021 catcaagaca cagggaaggg tgttgaagat gacgaccacg aacgtgtttc ggttcggcgg
     1081 gatcttgtgc gcggtatgtg cggtgggggc cgcgcgtgcc gaggagccgg cgagcgccgc
     1141 ggagatgcgg agcctcgacg agcaagtaca agagatcaag accgacgtgc tggggatcgc
     1201 ggcggagctc tcccgtctcg aggagaagct gctctatccc tcgggcacgc aaatcgcgat
     1261 cttcgtgtcg atcgccgagg gcgagacgtt ccgtctcgac tcgatgcaga tcgacatcga
     1321 cggcgcgctc gcgacgcatt acctctacgg cttcaaggag ctcgatgcct tgcagcacgg
     1381 aggggtgcag cgcgtctaca cgggcaacgt tccgaccggc gcgcatgcga ttgccgtctc
     1441 gatcaacggc aagaccaacg gcggcgacga attcaacagc accaaagagt tctcgttcga
     1501 caagggagtc gagccgaagc ttctcggtat cgcgctgaac ggccccgcgg caatcgagct
     1561 cggtgagtgg taacggcgca ctcgtaagcc acgagcgccg acccaccatg cccatgcgcg
     1621 tcaactcgct actcggccta ttcgcgtgcg gttttgccgc ggtcgccgtc gctggcccgc
     1681 cccccttcgg ggacttccaa gggtcgcgcc ccttcgggga cttccaaggg tcgccccccg
     1741 acgacgagga ggcggcgtcc cgagcccgcg actggtattt cggcgaggcg ctctactacg
     1801 cgcagcaggg ccactacttc gaagcgctcg agcggctcga cgcggagctc ggccaacatc
     1861 gcgccgtcga cgagcccgcg ctcgactcgc tgtaccgcga tttgcgcgag gcggaattct
     1921 cggtcggcga cttcgagctc aactaccgca tgcatcatcg cgcgggccgt gcaatcacgg
     1981 ccgtgctcga gggcgccgtc gacgaggaag tgcgcaacga cgccgcgtat cgcttggccc
     2041 gcatccactt tcagaagggt cagctcgagg acgccttgca ggcgctcgac cgcatcacgg
     2101 ggcgcgtgcc cgagggcatc gtcgacgaga tcgaattctt gcgcgccaac gtttacacgg
     2161 gcctcgggcg gcacgccgaa gcggcggaag tgttgcgccg cctgcagaac gccgacgggc
     2221 tcaagggctt cgcggcctac aacttaggaa tcgcgctgct cgaggacgag caccgccagg
     2281 acgcgctcgc gcagctcgac cgcgccggcc agattgcgag cggcgacgag cccgtgctcg
     2341 cgatccgcga caagtcgaac ttcgtgctcg gcacgttgct cgtcgaggcg caggacttcg
     2401 attccgcgaa acgctacttc gaccgcgtcc ggctcgacgg tccgttctcg aacgcggcgt
     2461 tgctggcgtc gggctgggcc gcaacggccg ccaacgacta tgagcgcgcc gtcgtgccgt
     2521 ggtcgctgct cgcgcagcgc gaggtcacgg acgcggccgt gcaggaggcg ttgatcgcgc
     2581 tgccgtacgc gtacggcaag ctcgacgtcc acggccgcgc cgcgctgctc tacgcccacg
     2641 ccctcgagtc gttcgggcac gagatccaaa ggctggacga ttcgctgacg agcatccgcg
     2701 acggcaagtt cctgaaggtg ctcgtgcgcg aagaggtccg gcaggacaag gactgggtca
     2761 tccgcttgcg ctcgctgccg gagtcgccgg agacctacta cttgatggag ctgctcgcgt
     2821 cgcacgattt ccagacggcg ctgcagaact acctcgacct cgaagacctg cggcgcaagc
     2881 tcgcgtcgtg ggacgtgagc ttcgactcgt tcgacgacct cgtcggcctg cgccgcggct
     2941 actacgagcc gcagctgccc ggcgtcgacg ccgatttccg cgagctcgac tcgcgcatgc
     3001 ggctgcggct cgagcagcac gagctgctcg cgaagcgctt gcaagctcag ttgacggcgc
     3061 cgcggccaga gctgcttgcg acgggcgaag agcggatgac ggccacgcgg ctcgacgagc
     3121 tcgcggcggc gctcgaggac gatacgagcc cgacggcgga cgccttacgg cagcgcatcg
     3181 cgcgcttgcg aggcacgctg tcgttcacgc tgcgcaccga gtatcacgag cggctcgacg
     3241 tgttcgcgaa gcatcttcgc gacctcggta aagccatcga
//
LOCUS       DATLEE010000521         4648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5068937, whole genome shotgun sequence.
ACCESSION   DATLEE010000521 DATLEE010000000
VERSION     DATLEE010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5068937"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1651)
                     /locus_tag="VM692_11245"
     CDS             complement(47..1651)
                     /locus_tag="VM692_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HUQ52788.1"
                     /translation="MIAVWIAGALLLGLAVSRIGLPPLVGFLASGFVFAALGMERTPM
                     LEDLAHAGVLLLLFAVGLKLRIKTLLRFEVWGTAIAHLGVTGLAGIAILSSGTGLPVG
                     LAFALAVAFGFSSTVLAAKVLEHNKELRAVHGRVAIGILIVQDVVAVVLLAFMSLETP
                     SPYALLLLFLPLARPAISWLLERCGHGELLVLFGAALAIGGGEGFAALGLSAELGALL
                     LGTMLADHKRSQELTTVLWGLKELFLVGFFLNIGFSGPPTWQALQDAIWLIVLLPLQG
                     VIFFLLLIGLGLRARTSFLTAVSLTTYSEFALIVVDVGVNNGLLGADWVVITALAVAL
                     SFVGAAPFNAFAHELYQWASPWLERLERDKRHPDDEPLSLGRAEILVVGMGRVGSGAY
                     DYLRQLNENIVGIDSDPAKIESNVRAGRRVAYADAEDPSFWQLLNVDRLRAIMFAVPD
                     LQAKVSAARELRKRGYRGLLSATHLYPEERDPIIEAGCDVTYNYYTEAGVGFARHTYD
                     TLRPPSAAAGGAIVKTEQPEKVRQFQ"
     gene            1847..2677
                     /locus_tag="VM692_11250"
     CDS             1847..2677
                     /locus_tag="VM692_11250"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52789.1"
                     /translation="MDRLQVWWDQLITVFSETFSSVITYLPVVLSAIAVLLIGWLLAH
                     LLRGLVRRALESMDWLFARLMPRSAGRSETLTRATSRAISTVVFWIILLVFAATALRI
                     LGGSLFERWTENLLAYLPQAIGGVIIIVIGFTGGTLARHILEQASVGLGVGQSSLLGR
                     LAQAVIVISCIVIGIDQLGVNVNFLIQLTTVTAAAVFGGIALVFALGTRQHLANLIGA
                     HYARKHYAPGDHVKIGGFQGRIVEIADGCVFIETDVGDVSVPGQHFSAEPFVKVRKGA
                     "
     gene            2674..3501
                     /locus_tag="VM692_11255"
     CDS             2674..3501
                     /locus_tag="VM692_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUQ52790.1"
                     /translation="MSGREELVAAYLRLHPESAARLLESMPTDEANAVLNAVDVATAA
                     PVVGHMLPTYAAQCFETQPPSDSALLLERLGSQESVAVLRHLRSEHREAVLNALGPQW
                     LMAFKLLLSYPTNTVGAWVEPRVLTLADDCTVGEARDRIARGEQVTQARIYVLDRARR
                     LRGAVRGLALLQVPNRKKLASILEPADALWARDALATAQEHSVWERHTEAPVVNRHEE
                     FIGVISYADLRRAFRQVNHVAGPQSDREIAEVTELIAIGAGSLWRSLGDLIGTDRRR"
     gene            3506..>4648
                     /locus_tag="VM692_11260"
     CDS             3506..>4648
                     /locus_tag="VM692_11260"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HUQ52791.1"
                     /translation="MEELGRNLALDRVSRQFLLEHPAQAADVIDGFGRAVAVELLAEH
                     QADTLATIWRHLSPARIDEVVPALPEKLLKQALHVLDPGVLALSLQRISDESRNRCLA
                     LLPKRAEAEVRRLMDYPVGSAGRVMDARILSFRGEQPAAMTLEQLRQSKPKDLRQLFI
                     VDAERRLTGVVDIQDLLIAEPTDLLKEIARPTSATVSPFDDRDEVAEKLRDFQLDVLP
                     VLDIHGRLIGAIRHEALLKTLEEAASVDIQMMVGAGREERALSEPQFAVRKRLPWLQI
                     NLATAFLAAAVVGLFESTIAPFTALAVLLPVVAGQSGNAGAQALAVTMRGLALREIST
                     RHWFRVLFKEMRVGVVNGLAIAATTGIGVLLWSGNSGLALVICIAMV"
BASE COUNT          777 a         1565 c         1550 g          756 t
ORIGIN      
        1 agcggccgcg ccgacacctt atatatgcgc tgccaggcgc atcaggtcac tgaaactgcc
       61 ggaccttctc gggttgttcc gttttgacga ttgcgccgcc cgccgcggcg gacggcggcc
      121 gcagcgtgtc gtaggtgtgg cgcgcgaagc cgaccccggc ctcggtgtag tagttgtagg
      181 tcacgtcgca ccccgcctcg atgatcgggt cgcgttcttc gggatataag tgcgtcgcgc
      241 tcagcagccc gcggtagccg cgcttgcgca gctcgcgcgc cgcggagact ttcgcctgca
      301 agtcgggcac cgcgaacatg atcgcgcgta gccgatcgac gttcagcagc tgccagaaac
      361 tcggatcttc ggcgtcggcg taggcgacgc ggcgccctgc acggacattc gactcgatct
      421 tggccgggtc gctgtcgatg ccgacgatgt tttcgttgag ctgccgcaga taatcgtagg
      481 cccccgagcc gacgcgtccc atgccgacga cgaggatttc ggctcgaccc agcgagagcg
      541 gctcgtcgtc gggatgccgc ttgtcgcgct cgagccgctc gagccacggg ctcgcccatt
      601 gatagagctc gtgcgcgaac gcgttgaatg gcgcggcgcc gacgaacgac agcgcgacgg
      661 cgagcgccgt gatgacgacc cagtcggcac cgagcaagcc gttgttgacg ccgacgtcga
      721 cgacgatcag cgcgaactcg ctgtacgtcg tcaggctgac agccgtcaga aagctcgtgc
      781 gcgctctcag gcccaagccg atcagcagca ggaaaaagat caccccttgt agcggcagca
      841 gcacgatgag ccagatggcg tcctggagcg cttgccacgt cggcgggccg ctgaacccga
      901 tgttcaagaa gaagccgacg aggaacagct ccttcaagcc ccagagcacc gtcgtgagct
      961 cctgcgagcg cttgtgatcg gcgagcatcg tgccgagcaa cagcgcgccg agctcggcgc
     1021 tcaacccgag cgccgcaaac ccttcgccgc caccgatcgc gagggcggcg ccgaacagca
     1081 cgagcagctc gccatggccg cagcgctcga gcagccacga gatggccggc cgcgcgagcg
     1141 gtaggaacag cagcaacaac gcgtacgggg acggcgtctc gaggctcatg aacgcgagca
     1201 ggacgacggc gacgacgtcc tgcacgatca aaatgccgat cgcgactcgg ccatggacgg
     1261 cccgcagctc cttgttgtgt tcgagcacct tggcggcgag cacggtgctc gagaagccga
     1321 atgcgaccgc gagcgcgaac gccagcccga cgggcaaccc cgttcccgac gagagtatcg
     1381 cgatgccggc gaggcccgtt acgcccaaat gcgcgatcgc cgtgccccag acctcgaagc
     1441 gcagcagcgt cttgatgcgc aacttcaagc ccaccgcgaa cagcagcagc agcacgccgg
     1501 cgtgtgcgag atcctcgagc atcggcgtgc gctccatgcc gagcgccgcg aatacgaagc
     1561 ccgatgccag aaacccgacg agaggaggta ggccgatgcg cgaaaccgcg aggcccagca
     1621 gtagtgctcc ggcaatccaa acggcgatca tgccgattcc ccctcgctcc cattatgcgg
     1681 gcggccaaga gcgagtgctc gtgtttgacg cgggcaggga gcacgcagta gagaaacgca
     1741 ggatgcagtt tccgagaagt ccgtctcccg agccgcgcgt gagtcaagta cgatggcgcc
     1801 gtcggctgcc gtcggcgcag cggcggctta acgtctcgga gctcgcatgg atcgactgca
     1861 ggtatggtgg gaccagctga tcactgtgtt cagcgagacg ttttcgagcg tcattaccta
     1921 cctgcccgtc gtcctgtcgg ccatcgcggt gctgctgatt ggctggctgc tcgcgcacct
     1981 gctgcgcggc ctcgtgcgcc gcgcgctcga gagcatggac tggctcttcg cccgcctcat
     2041 gccgcgctcg gcgggacgca gcgagacttt gacgcgcgcg acgagccgcg cgatctcgac
     2101 tgtggtgttc tggatcatcc tgctcgtgtt cgccgcgacg gcgctgcgaa tcctcggcgg
     2161 gtcgttgttc gagcgttgga ccgagaacct gctcgcgtac ctgccgcagg cgattggcgg
     2221 cgtcatcatc atcgtcatcg gcttcacggg cggcacgctg gcgcgccaca tcttggagca
     2281 agcgtccgtc gggctcggcg tcggccaaag cagcttgctg ggccggctcg cgcaagccgt
     2341 gatcgtcatc agctgcatcg tcatcggcat cgatcagctc ggcgtcaacg tcaatttttt
     2401 gattcagctg acgacggtca cggcggcagc cgtgttcggc ggcatcgcgc tggtgttcgc
     2461 gctcggcact cgccagcacc tcgcaaatct catcggcgcg cactacgcgc gcaagcacta
     2521 cgctcccggt gaccacgtga aaatcggcgg cttccagggc cgcatcgtcg agatcgcgga
     2581 cggctgcgtg ttcatcgaga ccgacgtcgg cgatgtctcc gtgccgggcc agcacttcag
     2641 cgccgagccg ttcgtgaaag tcaggaaggg cgcgtgagcg gtcgcgaaga gctcgtcgcc
     2701 gcgtatctgc gcctgcatcc cgagagcgcg gcgcgcttgc tcgagtcgat gccgaccgat
     2761 gaagcgaatg ccgtgctgaa cgccgtcgac gtggcgaccg ccgcacccgt ggtcggtcac
     2821 atgctgccga cctacgccgc gcaatgcttc gagactcagc ccccgtccga cagcgcgctg
     2881 ctgctcgagc ggctcggcag ccaggaatcg gtggccgtgt tgcggcactt gcgctccgag
     2941 caccgcgaag ccgtgctgaa tgcgctcggc ccgcaatggc tcatggcgtt caagctgttg
     3001 ctgagctatc cgaccaacac ggtcggcgcg tgggtagagc cgcgcgtgtt gacgctggcc
     3061 gacgactgca cggtcggcga ggcccgcgac cgcatcgcgc gcggcgagca ggtcacgcaa
     3121 gcccgcatct atgtgctcga tcgcgcgcgc cggctgcgcg gcgccgtgcg cgggctcgcg
     3181 ctgttgcaag tgccgaaccg caagaaactc gcttcgatcc tcgagcctgc ggacgcgtta
     3241 tgggcgcgcg acgcgttggc aacggcgcag gagcacagcg tctgggaacg ccacacggaa
     3301 gcgcctgtcg tcaaccgcca cgaggagttc atcggcgtga tctcgtacgc ggatctgcgc
     3361 cgcgcgtttc gccaggtcaa ccacgtcgcc ggaccgcaga gtgatcgcga gattgccgag
     3421 gtcaccgagc tcatcgcgat cggcgccggc agcttgtggc gcagcctcgg cgacttgatc
     3481 ggcaccgatc ggcgacgctg acgtcatgga agagctaggc cgcaacttag ccctcgaccg
     3541 agtgagccgg cagttcctgc tcgagcaccc cgcgcaagcc gccgacgtca tcgacggctt
     3601 cggccgtgcc gtcgccgtcg agctgctcgc cgagcaccag gccgacacgc tggctacgat
     3661 ctggcggcac ctgtcgccgg cgcgcatcga cgaggtcgtg cccgcgttac ccgagaagct
     3721 gctgaagcag gcgctgcacg tgctcgatcc cggcgtgctc gccctgtcgc tgcagcgcat
     3781 ctccgacgaa tcgcgcaatc gctgcctcgc actgttgccg aagcgcgccg aggccgaggt
     3841 gcgccggctc atggattacc cggtcggctc ggccggccgt gtcatggatg cgcgaatcct
     3901 gagctttcgc ggcgagcagc ccgctgccat gacgctcgag caactgcggc agagcaagcc
     3961 gaaggatctg cgccaactgt tcatcgtcga cgccgagcgc cggctcacgg gcgtcgtcga
     4021 catccaggat ctcctgatcg ccgagccgac ggacctcttg aaggagatcg caaggcccac
     4081 gagcgcgaca gtgtcgccgt tcgacgatcg cgacgaggtg gccgagaagc tgcgcgactt
     4141 ccagctcgac gtgctgcccg tcctcgacat tcacggccgg ttgatcggcg ccatacgcca
     4201 cgaggcgttg ctgaagaccc tcgaggaagc ggcgtccgtc gacatccaga tgatggtcgg
     4261 cgccggccgc gaggagcgcg cactctcgga gccgcagttc gccgtgcgca agcggttgcc
     4321 ctggttgcag atcaacctcg cgactgcgtt tctcgcggcc gccgtcgtcg ggctgttcga
     4381 atcgacgatc gcgccgttca cggcgctcgc cgtgctcttg ccggtcgtag cagggcaatc
     4441 gggcaacgcg ggagcccaag ccctcgcggt cacgatgcgc gggctcgcgc tgcgcgagat
     4501 ctcgacgcgg cattggtttc gggtgctgtt caaggagatg cgcgtcggcg tcgtcaacgg
     4561 gctcgcgatc gcggcaacga ccggcatcgg cgtgctgctc tggagcggga actccggcct
     4621 cgcgctcgtg atctgtattg ccatggtc
//
LOCUS       DATLEE010000522         2520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7747824, whole genome shotgun sequence.
ACCESSION   DATLEE010000522 DATLEE010000000
VERSION     DATLEE010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7747824"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VM692_11265"
     CDS             <1..554
                     /locus_tag="VM692_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HUQ52792.1"
                     /translation="DLRPYIDSVADAGVKTFISIGGQRRNMPYEQAADNAVAFLNRIK
                     GQLEDRGVTIAIENMNNRRTDANFGRVDQTFGRWAWGVGVCERVNSPNVKLVCDLYHL
                     QIMDGDIAQNLRDTIQWIAHFHTAGVPSRNEIDFSQEMNYRYIAEVIADLPFTGYVSH
                     EWRPTPGRDPLRSIAEALEIMDV"
     gene            602..>2520
                     /locus_tag="VM692_11270"
     CDS             602..>2520
                     /locus_tag="VM692_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUQ52793.1"
                     /translation="MNSSSLGRGSRFAALVTALAASVVWCAAGAQEARRLAEGFIGGV
                     VTSTRGPEAGVWVIAETDELKTPFIKIVVTDDAGRYVLPQLPTATYNVWVRGYGLADS
                     DRVEGRPGDTALNLTAKVASSPAEAAKVYPGNYWLSLLQPPAPDQFPGTGESGNGIPP
                     AMATQAHWIFNIKSGCNFCHQLGNQLTRSLGHMDHLGFKSPEEAWIYRTQLGVRGSSM
                     AGTMAQWGVNAGARVLADWTTRIANGELPSAPPRPRPGPERNVVVTMWDWGVDSSFMH
                     DEISTDKNDPTVNANGPVYAVSAGHGKLTVVDPIDNDSYELTIPTREDARQVQSRFPP
                     PAMPSNFWGMQHIWGLENPADPHNPMMDRKGRVWMTSKIRNEEPGWCKEGSSHKFAQW
                     YPLTFSNRQASFYDPATATFTLIDTCFATHHLQFDNDPDQTIYFNELLGPIVGWVNTR
                     EYDRTGDEQATQGWCPQVVDSNGDGKITKPWNVPGQPADPRRDTEVRLNLYSIIPSPV
                     DNSVWGASEETPGYIVRVDLGDNPPQTCMTEVYKVPEPGIDPRGIDVDSNGVVWTALA
                     ASSHLASFDRTKCRVFNGPRVVEGTQCEAGWKLYLSDGPKFKGTNIPSDFHYYNWVDQ
                     HDIAGFGANTPIATGS"
BASE COUNT          480 a          832 c          804 g          404 t
ORIGIN      
        1 aggaccttcg cccgtacatc gattccgtcg cggacgccgg cgtgaaaacg ttcatctcga
       61 tcggcggtca gcgccgcaac atgccgtacg agcaggctgc cgacaacgcg gtcgcgttcc
      121 tgaatcgcat caaaggtcag ctcgaggatc gcggcgtcac gattgcgatc gagaacatga
      181 acaaccgccg gacggatgcc aacttcgggc gcgtcgatca gaccttcggc cgctgggcgt
      241 ggggcgtcgg cgtctgcgag cgcgtcaact cgccgaacgt caagctcgtc tgcgatctct
      301 accacttgca aatcatggac ggcgatatcg cgcagaactt gcgcgacacg attcagtgga
      361 tcgcgcattt ccatacggcc ggcgtgccgt cgcgcaacga gatcgacttc agccaggaga
      421 tgaactaccg ctacatcgcg gaagtcatcg ccgacttgcc gttcacgggt tacgtgagcc
      481 acgagtggcg gccgacgccg ggccgcgatc cgctgcgcag cattgcggaa gcgctcgaga
      541 tcatggacgt ttagaatcgc gctgctcgcg caacttgatc gcataacttc aggggacttg
      601 catgaatagc tctagcctcg gtcgcggcag ccgtttcgct gcgctggtta cggctctcgc
      661 cgcgtcagtc gtgtggtgcg ccgcaggcgc gcaagaagcc aggcggctag ccgagggatt
      721 catcggcggt gtcgtcacga gcacgcgcgg ccccgaggcc ggcgtctggg tcatcgccga
      781 gaccgacgag ctcaagacgc cgttcatcaa gatcgtcgtg accgacgatg ccggccgcta
      841 cgtgctgccg cagttgccca cggcgaccta taacgtctgg gtgcgcggct acgggctcgc
      901 ggattccgat cgcgtcgaag ggcgccccgg cgatacggcc ctgaacctca cggcgaaggt
      961 cgcgagctcg ccggccgaag cggccaaggt gtatcccggg aactactggc tgtcgctgct
     1021 ccagccgccc gcgccggacc agttccccgg caccggcgag agcggcaacg gtattccgcc
     1081 ggccatggcg acgcaggcgc actggatctt caacatcaag agcggctgca acttttgtca
     1141 ccagctcggc aatcagctga cgcgctcgct cggtcacatg gatcacctcg gcttcaagtc
     1201 gcccgaggaa gcctggattt atcgcacgca gcttggcgtg cgcggcagct cgatggccgg
     1261 cacgatggcg cagtggggcg tgaatgccgg cgcacgcgtg ctcgccgact ggacgacgcg
     1321 catcgcgaac ggcgagctgc cgtccgcgcc gccgcgaccc cggccgggtc ccgagcgcaa
     1381 cgtcgtcgtc acgatgtggg attggggcgt cgactcgtcg ttcatgcacg acgagatttc
     1441 gaccgacaag aacgacccga ccgtaaacgc caacggcccc gtgtacgccg tgtcggccgg
     1501 gcacggcaag ctcacggtcg tggacccgat cgacaacgac tcttacgagc tgacgatccc
     1561 gacgcgcgag gatgcgcggc aagtgcagtc gcggttcccg ccaccggcga tgccgtcgaa
     1621 tttctggggc atgcagcaca tctgggggct cgagaatccg gccgacccgc acaatccgat
     1681 gatggaccgc aagggccgcg tctggatgac gtccaagatc cgcaacgagg aacccggctg
     1741 gtgcaaggaa ggctcgagcc acaaattcgc gcagtggtat ccgctgacct tcagcaatcg
     1801 ccaggcgtcg ttctacgatc ccgctacggc tacgttcacg ctgatcgaca cgtgcttcgc
     1861 gacgcatcac ctgcaattcg ataacgaccc cgatcagacg atctacttca acgagctgct
     1921 cgggccgatc gtcggctggg tcaacacgcg cgagtacgac cgcacgggcg acgagcaggc
     1981 gacgcagggc tggtgcccgc aagtcgtcga cagcaacggc gacggcaaga tcacgaagcc
     2041 gtggaacgta cccggccagc ccgcggatcc gcggcgcgac acggaagtgc ggttgaacct
     2101 ctactcgata attccgagcc ctgtcgacaa ctcggtctgg ggcgcgtccg aggagacgcc
     2161 gggctacatc gtgcgcgtcg atctcggcga caacccgccg caaacgtgca tgaccgaggt
     2221 ctacaaggtt cccgagcccg gcatcgatcc gcgcggcatc gacgtcgact cgaacggcgt
     2281 cgtgtggacg gcgctcgcgg cgagcagcca cctcgcgagc ttcgatcgca cgaagtgccg
     2341 tgtcttcaac ggcccgcgcg tcgtcgaagg cacgcagtgc gaagcaggct ggaagctcta
     2401 tctatcggac ggcccgaagt tcaagggcac gaacattccg tccgacttcc actattacaa
     2461 ctgggtcgat caacacgaca tcgccgggtt cggcgcgaac acaccgatcg cgacgggctc
//
LOCUS       DATLEE010000523         2150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5071767, whole genome shotgun sequence.
ACCESSION   DATLEE010000523 DATLEE010000000
VERSION     DATLEE010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5071767"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VM692_11275"
     CDS             <1..762
                     /locus_tag="VM692_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52794.1"
                     /translation="KPDLTASWRSDGNKFFIWRYGNRRCAPTQLDGCTPAWNQTLDFE
                     FEAASRFGPNRPLYKPEHWDEVIYKDMWTNREDPIMTCQPMGIPRQGPPGRIFATTDD
                     VTLIYGRGGDGGGGYSDFRVIATDGRPFDERAILQTKYTGHSVGHWDGDTLVIESVGL
                     TPETWLARGGFFHSDQMKVFERFTRQGDQLLYEVTVEDPGVLLQPWTLKPMLMTAAAS
                     GPPTGGPGGNASLIGSERGACQQEELDDIVLQIRH"
     gene            complement(889..1140)
                     /locus_tag="VM692_11280"
     CDS             complement(889..1140)
                     /locus_tag="VM692_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52795.1"
                     /translation="MSKLERSLACVALVLMASATSAVAQDISDAADLTAVIALNGKPC
                     GKVVNAAKRGDNDYVATCEDGNRYHVYVKDGRVVVDKTS"
     gene            complement(1144..1581)
                     /locus_tag="VM692_11285"
     CDS             complement(1144..1581)
                     /locus_tag="VM692_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52796.1"
                     /translation="MANEPRAPRAAPATPPPSVAAAPPAPAESAPVAAAPPAPAAGTL
                     DFASLGTRLRETKAIGVLTKLAVKNQADDLLEKFRAYHKRQSAVTLTDLRSSYDLLVL
                     KILSLVQDGDPPLAREIDRSRAAIWDILTDQRKFVESNLMSGA"
BASE COUNT          354 a          710 c          730 g          356 t
ORIGIN      
        1 aagcccgacc tgacggcgag ctggcgcagc gacggcaaca agttcttcat ctggcgctac
       61 ggcaatcgcc gctgcgcacc gacgcagctc gacggctgca cgccagcctg gaatcagacg
      121 ctcgatttcg agttcgaagc ggcctcgcgc ttcgggccga atcgcccgct gtacaagccc
      181 gagcactggg atgaagtcat ctataaggac atgtggacga accgcgaaga tccgatcatg
      241 acctgtcagc cgatgggcat tccgcgtcag ggaccgcccg ggcgcatctt cgccacgacg
      301 gacgacgtca cgttgatcta cggccgcggc ggcgacggcg gcggcggtta ttccgacttc
      361 cgcgtgatcg cgaccgacgg ccgtccgttc gacgaacgcg cgattctgca gaccaagtac
      421 acggggcact cggtcggcca ctgggacggc gacacgctcg tcatcgaatc cgtcggcttg
      481 acgccggaga cgtggctggc gcgcggcggc ttcttccact ccgatcagat gaaggtcttc
      541 gagcgcttca cgcggcaggg cgatcagctg ctctacgagg tcaccgtcga ggatccgggc
      601 gtgttgctgc agccgtggac gctgaagccg atgctgatga ccgcggccgc tagcggtcca
      661 ccgacaggcg gtcccggcgg taacgcctca ttgattggtt cggaacgcgg cgcctgtcag
      721 caagaagagc tcgacgacat cgtgctgcag atccgccact agccatctgc gcaagcgagc
      781 gccgggcgtc caacaggcgt ccggcgctcc gatcctgcac ccagcgactt acaagccggc
      841 caccgcagtc cgcccgacgg cgcgccgaaa gtgcgctcgt cgctccagct agctcgtctt
      901 gtcgacgacg acgcgaccgt ccttcacgta cacgtgataa cgattgccgt cctcgcacgt
      961 cgcgacgtag tcgttgtcgc cgcgcttcgc cgcgttgacg actttgccgc acggtttacc
     1021 gttcagcgca atgacggccg tcagatccgc agcgtcgctg atgtcctgtg cgaccgcgga
     1081 cgtcgcgctc gccatcagca cgagagctac gcaagccagc gatcgttcga gtttgctcat
     1141 cgatcaagct cctgacatca agttcgattc tacgaatttg cgctggtccg tgaggatgtc
     1201 ccagatcgcc gcacgcgacc gatcgatctc acgcgccaac ggcggatcgc cgtcctgaac
     1261 cagcgacaag atcttcagga cgagtaagtc gtacgagctg cgcagatcgg taagcgtcac
     1321 cgcgctctgt cgcttgtgat acgcgcgaaa cttctcgagc agatcgtcgg cttggttctt
     1381 caccgcgagc ttcgtcagca cgccgatggc tttcgtctca cgaagccgtg tgcccagcga
     1441 cgcgaagtcg agcgtgcccg ccgccggcgc gggcggtgca gccgcgacgg gcgcgctctc
     1501 cgccggcgcg ggcggtgcag ccgcgacgga cggaggcgga gttgctggcg ccgcgcgcgg
     1561 cgctcgcggc tcgttcgcca cgctcggcgc agcgggcgcg cttggcgctg cagcgcgcgc
     1621 cggcggcgcg ggtggtgggc gattcgcagc ggctggcggg gccggcggtt gcggcgcggc
     1681 gggcgcagcg cgagtcggcg ctgcgggtgc cgctggtgac ggcgttgcgg ccggcgacgg
     1741 ctccgaattc gcggctagcg gcggcgcggg tgccgcagaa ggtgcagccc gcgccggtgc
     1801 cggttcaaca ggtacggttg cggcggttgc cgtacgcgac gcaggtgcgg gtgtgtcgga
     1861 ctgcgtcgaa gcggtgtgca gccccgagca acccgtcgcg ccgatgcaca gcacagcgat
     1921 cgcgcattgc gacagcgagt tgcgaggcat catgcgtcct cgctccgctc gatgaccctc
     1981 gccgacaaaa agcaatgagc tcgcatacga ctagaggata gctctccgcg cgagagcggt
     2041 ggagaaaata tctctcgacg agacggttgg cgttcagcgt ggattcactc gggtcgtgcg
     2101 gcgagcgctc acaaaagcgt gctgtgtgcc cgccgccgct ccggaggtcg
//
LOCUS       DATLEE010000524         3827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2838355, whole genome shotgun sequence.
ACCESSION   DATLEE010000524 DATLEE010000000
VERSION     DATLEE010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2838355"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..154
                     /locus_tag="VM692_11290"
     CDS             17..154
                     /locus_tag="VM692_11290"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HUQ52797.1"
                     /translation="MSEIFDAEAPYAARGCVAQAWSVAELLRCAVYAARDREALANAD
                     D"
     gene            complement(151..1734)
                     /locus_tag="VM692_11295"
     CDS             complement(151..1734)
                     /locus_tag="VM692_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HUQ52798.1"
                     /translation="MSRAPWWQHGAIYQLLVPSFLDTDNDGLGDLDGIAARLDYLEWL
                     GVNAVWLSPCYPSPLRELGYDVSDYRNIEPRFGSLATFDRLLDEAHQRDLHVILDWVG
                     NHTSAQHPWFRESRSSRNNARRNWYLWRDARPDGSPPNNWISVFGGSVWQWDSTTEQY
                     YLHTFLDSQPDLNWREPDVRVAMFDALKFWLDRGVDGFRLDAAPLFFKDTEWRDNPVN
                     PDYQSGELPDSSLLPKYTRNQPGLHELFVELRAFVDRYPGDRVLLGEFYTPIEELIGF
                     YGTGAPELHLPLNLTLTYTKWQPSAVGRTIAEYQGRVSGRGWPTTTLDTHDQERIVVR
                     AGLTQARVAALLLLTQRGTPTIYYGDEIGMRGVAIPPEQAVDPQGRRTGRNRDPTRTP
                     MQWSSENYAGFSSVEPWLPVGDDRISANVASQSRDAGSLLTLYRRLLELRAAEPALRD
                     GVHEPLAAGPALVAYRRRNATRRLLVVLNLVHEPTSYALGDDGPGRVLLSSVLDREGE
                     RVVDHVMVRADEGLLIALD"
     gene            1835..2320
                     /locus_tag="VM692_11300"
     CDS             1835..2320
                     /locus_tag="VM692_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUQ52799.1"
                     /translation="MTIEALMSRHVHYCRPDHTLDYAASQMWDHDCGCVPVCVDDGEP
                     RVIGMLTDRDICMAALFQGRPLYELKVADAMSRDIHVCEVDARPEDVELTMRELRVRR
                     LPVTDVGGGLVGIVSLADLARAAVGGAGADGFDGLSESNVGHTLAAICAPGEPIATPP
                     D"
     gene            2495..3010
                     /locus_tag="VM692_11305"
     CDS             2495..3010
                     /locus_tag="VM692_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUQ52800.1"
                     /translation="MERVERSVEVDCPVRTVYNQWTQFEEFPRFMSGVKEVTQIDDTH
                     VHWHAEVWGKDKEWDAEITEQVPDQRISWRSTSGEENAGTVRFEPLPGNRTLVRLAMG
                     YEPKGAVENAGDTLGLFTSHVETTMKDFKHYIEERQVADGAWRGEVHGGRRQPGASDR
                     RPSDAPRRSGQ"
     gene            3022..3258
                     /locus_tag="VM692_11310"
     CDS             3022..3258
                     /locus_tag="VM692_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52801.1"
                     /translation="MKHIRTPDGVCLQCFNSQQRFAFPFLVYCEHSESLALVRSAEES
                     TTFHCKPAQLAGVLKKISSVRGGADAIPPARDRL"
     gene            3227..>3827
                     /locus_tag="VM692_11315"
     CDS             3227..>3827
                     /locus_tag="VM692_11315"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HUQ52802.1"
                     /translation="MRYHLLATDYDGTLATDGHVDAATLDALNGILATGRRLVLVTGR
                     NLDELLAVHPSLEIFERVVAENGALLYRPATKERRKLAEPPPPAFIAALRQRGVAPLD
                     VGEVIVATREPHEAVVLETIRDLGLELQVIFNKGAVMVLPTGVNKATGLTAALEELKI
                     SPHNVVGVGDAENDHSFLKLCERSAAVGGALPALRDTADL"
BASE COUNT          679 a         1280 c         1256 g          612 t
ORIGIN      
        1 cgggtgcatc ggcagcatca gcgagatctt cgacgccgag gcaccgtacg cggcccgtgg
       61 ctgcgtcgcc caggcctgga gcgtggccga gctgctgcgc tgcgccgttt acgccgcgcg
      121 cgaccgcgaa gcgctcgcca acgccgacga ctagtcgagt gcgatcagca gcccctcgtc
      181 ggcgcgcacc atgacgtgat cgaccacgcg ctcgccttcg cgatcgagca cgctcgacag
      241 cagcacccgc cccggtccgt cgtcgcccag cgcgtagctc gtcggctcgt gcacgaggtt
      301 cagcacgacg agcaagcgtc gagtcgcatt gcgccgccga taagccacga gtgcggggcc
      361 ggccgcgagc ggctcgtgca ccccgtcgcg caacgcgggc tcagccgccc gcagctcgag
      421 cagccgccga tagagcgtca gcaatgagcc cgcatcgcga ctctgacttg cgacgttggc
      481 gctgatccga tcgtcgccga ccggcaacca cggctccacg ctcgagaacc cggcgtaatt
      541 ctcgctgctc cattgcatcg gcgtgcgcgt cggatcgcgg ttgcgcccgg tgcggcgccc
      601 ttgtggatcg acggcttgct cgggaggaat cgcgacgccg cgcatgccaa tctcgtcgcc
      661 gtagtaaatc gtcggcgtgc cgcgctgagt cagcagcaac agcgcggcga cgcgcgcttg
      721 agttagaccc gcgcgcacga cgatgcgctc ctgatcgtgc gtatcgagcg tcgtcgtcgg
      781 ccaaccgcgt cccgatacac gcccttggta ctccgcgatc gtgcggccga cggcgctcgg
      841 ctgccatttc gtatacgtca gcgtcagatt cagcggtagg tggagctccg gcgcgcccgt
      901 cccgtagaag ccgatgagct cctcgatcgg cgtgtagaac tcgccgagca gcacgcgatc
      961 gccgggatac cgatcgacga aagcccggag ctcgacgaac agctcgtgca gtccgggctg
     1021 attgcgcgtg tatttcggca gtaacgagct gtcgggcagc tcgccggatt gatagtcagg
     1081 attgaccgga ttgtcgcgcc actcggtgtc tttgaagaat aacggcgccg catcgagcct
     1141 gaaaccgtcg acgccgcggt cgagccagaa tttcaacgca tcgaacatgg cgacgcgcac
     1201 gtccggctcg cgccaattca aatcgggctg cgagtccaaa aacgtgtgca agtagtactg
     1261 ctccgtggtg ctgtcccact gccacacgct gccgccaaac acgctgatcc agttgttcgg
     1321 cggcgagccg tcgggtcgcg cgtcgcgcca tagataccaa ttgcgccgcg cattgttgcg
     1381 actcgagcgc gactcgcgaa accacgggtg ttgcgccgac gtgtgattac cgacccaatc
     1441 gagtatgacg tgcaggtcgc gctggtgcgc ctcgtcgagc agccggtcga acgtcgcgag
     1501 cgagccgaac cgcggctcga tgttgcggta atcgctgacg tcgtagccga gctccctcag
     1561 cggcgacgga taacacggcg acagccagac ggcattgacg ccgagccatt cgaggtagtc
     1621 gagacgcgcc gcgatgccgt ccagatcccc aagaccgtcg ttgtccgtgt cgagaaacga
     1681 cggcacgagc agctgataga tggccccgtg ctgccaccac ggcgctcggc tcacgcgctg
     1741 tccctcgacg cctgcattcg agctacggca gcaatacgca tgccagcccc gcgggcacga
     1801 ggcttgcaac gtcggccccc cgaggaggat cgccatgacg atcgaagcgc tcatgtcgcg
     1861 gcacgtgcac tactgccgcc ccgaccacac gctcgactat gcagcgtcgc agatgtggga
     1921 ccacgactgc ggctgcgtgc cggtctgcgt cgacgacggc gagccgcgcg tcatcggcat
     1981 gttgacggac cgcgatatct gtatggcggc gctgttccaa ggccggccgc tgtacgagct
     2041 caaggtcgcg gacgccatga gccgcgacat ccatgtctgc gaagtggacg cccgccccga
     2101 ggacgtcgag ctgaccatgc gcgagctcag ggttcgccgg ctgcccgtca ccgacgtggg
     2161 cggcgggctc gtaggtatcg tctcgttagc cgacctcgcg cgcgcggctg tcggcggcgc
     2221 cggtgccgac ggcttcgacg ggctctcgga gagcaacgtc ggtcatacgc tggcggccat
     2281 ctgcgctccc ggcgagccca tcgcgacacc cccggattag gccacttcgg ccccaccgca
     2341 ctcctttata tatagccgtt cggcctgtcg caattttccg ggccctcctt ccaattgctg
     2401 gaagcggctc ggcagcgaag gcgcccccgc tcccggaacg gttcttgcaa ttctttgcca
     2461 tccgaagtcg taccaacact caaggagaga ggaagtggaa cgagtagaac ggagcgtgga
     2521 ggtggattgc ccggttcgga cggtctacaa ccaatggacg cagttcgagg aattcccgag
     2581 gttcatgtcg ggagtcaagg aagtcaccca gatcgacgac acgcacgtgc actggcacgc
     2641 cgaggtttgg ggcaaggaca aggaatggga cgccgagatc acggagcaag tgcccgacca
     2701 acgtatctcc tggcgcagca cgagcggcga agagaacgcc ggcaccgtgc gcttcgagcc
     2761 gttgcccggc aatcgcacgc tggttcgcct cgccatgggc tacgagccga agggcgccgt
     2821 cgagaacgcc ggcgacacgc tcggcttgtt cacgtcgcat gtcgaaacca cgatgaagga
     2881 cttcaagcac tacatcgagg aacgccaagt tgcggacggt gcctggcgcg gcgaagtgca
     2941 cggcggccga cggcagcccg gagcgagcga ccggcgacca agcgacgcgc cgcgccgctc
     3001 gggccagtga aagtcgcgcg cgtgaagcac attcggacgc cggacggcgt ctgcttgcag
     3061 tgcttcaact cgcagcagcg gttcgctttt ccgttcctcg tttattgcga gcactcggag
     3121 tccctggcgc tcgtgcgctc cgcggaagaa agcacgacgt tccattgcaa gccggcacag
     3181 ctcgccggcg tgctgaaaaa gatttcgagc gttcgaggcg gtgcggatgc gataccacct
     3241 gctcgcgacc gactatgacg gcacgctggc gaccgacggc cacgtagacg cggcgacgct
     3301 cgacgcgctg aacgggattc tcgccacggg acgccggctc gtcctcgtca cgggccgcaa
     3361 cctggacgaa ttgctggcgg tgcacccgag cctcgaaatt ttcgagcgcg tcgtcgcgga
     3421 gaacggcgcg ctgctctacc gtccggcaac gaaagagcgc cgcaagctgg cggagcctcc
     3481 gccgccggct ttcatagcgg cgttgcgaca gcgcggcgtc gcaccgctcg acgtcggcga
     3541 ggtcatcgtc gcgacgcgcg agccgcacga agccgtagtg ctagagacga ttcgcgacct
     3601 cggcctcgag ctgcaggtca tcttcaacaa gggcgccgtg atggtgctgc cgaccggcgt
     3661 caacaaagca acggggctca cggccgcgct cgaggagctc aagatctcgc cgcacaacgt
     3721 cgtcggcgtc ggcgacgcgg agaacgacca ttcgtttttg aagctatgcg agcggtcggc
     3781 cgccgtgggc ggggcgttgc ccgcgctgcg cgacacggcg gacttgg
//
LOCUS       DATLEE010000525         4649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4306844, whole genome shotgun sequence.
ACCESSION   DATLEE010000525 DATLEE010000000
VERSION     DATLEE010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4306844"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2780
                     /locus_tag="VM692_11320"
     CDS             <1..2780
                     /locus_tag="VM692_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HUQ52803.1"
                     /translation="VVVPRTADEAVRAAELAAEAGVPLLPRGAGTSQCGQTVGAALVI
                     DSSKHLNRLIEVDAASASAWVEPGIVLDDLNARLRKEGLWYPVDVSTSAQATIGGMTA
                     NNSCGARSLRYGNMVHNVLQVETWLSTGETLTFGAVGSGPPSGPARYRELVEKIRALH
                     ARERDEIEARVPKLQRRVAGYNLDLASADAFNAAQLLVGSEGTLGLFRKIRLKLAPLP
                     TAKALGVVHFPTFYQAMDLTRHIVGLEPTAVELVDRTMIELAREIAAFRATMDKTIRG
                     EPDAILLVEFAGDEQAPQVAKLKRLSELMADLGLPGSVVEIGDASWQRDVWEVRKAGL
                     NIMMSMKGDGKPVSFIEDCAVPLEHLADYTDRLTRVFEKHGTRGTFYAHASVGCLHVR
                     PVLNLRTEGGARQMRAIAEEACALVKEYKGSYSGEHGDGLVRSEWIEPIYGARLTRAF
                     AELKQAFDPRGLMNPGKIVAATKMDDRSLFRFKPDYRTAPLATGLDWSEHEVGPENRG
                     RGFAAAVEMCNNNGHCRKFDAGTMCPSYRVTRDEQHVTRGRANSLRLALSGQLGDDAL
                     ASQQLHDTLALCVSCKGCKRECPTGVDMAAMKIEFLHHYHRRHPRSLRDRLVAHLPRY
                     ARTAARLAPLMNLRDRIPGVAKLSEALVGLAAKRTLPKWRRDYFRASEVPAPSAANDG
                     RDVVLFGDTFNTYFEPENLRAAAAVLTAGGYSVRVATAPDGRALCCGRTYLATGMLDE
                     ARAEARRLLDALAPLAARGVPIVGLEPSCILGLRDELKMLCPGTASRDVARAALTFEE
                     FLVRERAAGRWQRAFKRAPFKRALVHGHCHQKAFGAMPAVVAALQLVPDLTVEVVASS
                     CCGMAGAFGYEAEHLDVSLRMAEAALLPAVRNAGDDTVVVADGTSCRHQIHDGAQRNA
                     EHVARVLASVL"
     gene            2913..3200
                     /locus_tag="VM692_11325"
     CDS             2913..3200
                     /locus_tag="VM692_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52804.1"
                     /translation="MGNQNTALATTAAPPSTSLTALAEQLRDGPLHDLIQLQLKATEL
                     ADRLAASPADRIEDLERLVRLSLTAMEQFHAFTREFAAVLRELTDARRHPH"
     gene            3178..3618
                     /locus_tag="VM692_11330"
     CDS             3178..3618
                     /locus_tag="VM692_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ52805.1"
                     /translation="MPAATRTELYTRLVELCQEFRAHGDLECEVALDEHHTKFDEELS
                     EVVFRTVRELLTNVRQHAKATRVLVSSIGRRDGSIGILVADDGVGLPAHRRRGNPFDE
                     SGGIGLWSIDQRLRAFDAVLDVEAADGKGTRAMVILPGKLLVAD"
     gene            complement(3615..>4649)
                     /locus_tag="VM692_11335"
     CDS             complement(3615..>4649)
                     /locus_tag="VM692_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52806.1"
                     /translation="DAPGTTGYLLTGSVRGTADHARITVRLIEAGSGMQLWTAAYDES
                     LEIEQLPVLQEQVARDVAAIAAPYGPIFEAELARARRSVQAPKLRDCLVTYYDYRRRK
                     APEVHRDALLCFQNVSVLQPEVAQVWSGLAMLYVDNYAATFGRNGENELSAARESTAK
                     ALLLDPDDFLGNLALTRVQFFDGDAAFQQTIERTIALRPGNAQALAQGGFLLTVRGES
                     ERGLALLQRAKALSKTPPALYNLAYAVTSLRQHRYAEALSFAQKVDTQNWVMAQAIVA
                     AAAAHGGRDEIAAKAVRRLRELYPEFEAEALTNFERWRFDRDYYDVLVSGLKAAGLEL
                     REQAVPISGG"
BASE COUNT          760 a         1587 c         1578 g          724 t
ORIGIN      
        1 gcgtggtggt gccgcgcacg gccgacgagg ccgtccgggc ggcagagctc gcggccgaag
       61 caggcgtccc cctactgccg cgcggcgccg gcacgtcgca gtgtggccag accgtcggcg
      121 cggcgctcgt catcgactcg agcaaacacc tgaatcggct catcgaagtc gacgccgcga
      181 gcgcgagcgc gtgggtcgag cccggcatcg tgctcgacga tctgaacgca cgtttgagaa
      241 aagagggcct ctggtatccc gtcgacgtgt ccacgtcggc gcaagcgacg atcggcggca
      301 tgacggccaa caacagttgc ggcgcgcgct ccttgcgcta cggcaacatg gtgcacaacg
      361 tgctccaggt cgagacgtgg ctctcgaccg gcgagacgtt gacgttcggc gccgtcggct
      421 ccggcccgcc gtcggggccc gcgcgctatc gcgagctcgt cgagaaaatc cgcgcgttgc
      481 acgcacgcga gcgcgacgag atcgaagcgc gagtgccgaa gttgcagcgc cgcgttgcgg
      541 gctacaacct cgacctcgcg tccgcggacg cgttcaacgc cgctcagctg ctcgtcggct
      601 ccgaagggac gctggggctg ttccgcaaga tcaggctcaa gctcgcgccg ctgccgacgg
      661 cgaaggcgct cggcgtcgtg catttcccga ctttctatca ggcgatggac ctgactcggc
      721 acatcgtcgg gctggagccg acggccgtcg agctcgtcga tcgcacgatg atcgagctgg
      781 cacgcgagat cgcggcattc cgcgcgacga tggacaagac gatccgcggc gagccggacg
      841 cgattctgct cgtcgagttc gccggcgacg agcaggcgcc gcaggttgcg aagctgaagc
      901 gcttgagcga gctcatggcc gacctcgggc ttccgggcag cgtagtagag attggcgacg
      961 cgtcgtggca gcgcgacgtt tgggaggttc gcaaagcggg cctcaacatc atgatgtcga
     1021 tgaaaggcga cggcaaaccc gtgtcgttca tcgaggactg cgccgtgccg ctcgagcacc
     1081 tcgccgacta cacggaccgg ctgacgcggg tgttcgagaa gcacggcacg cgcggcacgt
     1141 tctacgcgca cgcctcggtc ggctgcctgc acgtgcgtcc cgtgctgaac ctgcgcaccg
     1201 aaggcggcgc acggcagatg cgcgcgatcg ccgaggaggc ctgcgcgctc gtcaaggaat
     1261 acaaaggctc gtactccggc gagcacggcg acgggctcgt gcggtccgag tggatcgagc
     1321 cgatctacgg cgcgcgactg acgcgcgcgt tcgccgagct caagcaagcg ttcgatccaa
     1381 ggggactcat gaatcccggc aagatcgtcg cggcgacgaa gatggacgat cggtcgctgt
     1441 tccgtttcaa gccggactac cgcacggcgc cgttggcgac ggggctcgac tggtccgagc
     1501 acgaagtcgg gccggagaac cgcggccgcg ggttcgccgc cgcggtcgag atgtgcaaca
     1561 acaacggcca ctgccgcaag ttcgatgccg ggacgatgtg cccctcgtat cgcgtgacgc
     1621 gcgacgagca gcacgtgacg cgcggccgcg ccaactcgct acgtctcgcg ttatcgggcc
     1681 aactcggcga cgacgcgctc gcatcgcaac aactgcacga cacgcttgcg ctctgcgtct
     1741 cgtgcaaggg ctgcaagcgc gaatgcccga ccggcgtgga catggccgcg atgaagatcg
     1801 agttcttgca tcactaccat cggcggcacc cgcgctcgtt gcgcgaccgg ctcgtggcgc
     1861 atctgccgcg ctacgcgcgg acggccgcgc ggctcgcgcc gctgatgaat ctgcgcgatc
     1921 gcatcccggg cgtcgccaag ctgtccgagg cgctcgtggg ccttgccgcg aagcgcacgc
     1981 tgccgaagtg gcggcgcgac tacttccgcg cgagcgaggt gcccgcgccg agcgccgcga
     2041 acgacggccg cgacgtggtc ctgttcggcg acacgttcaa cacttatttc gagcccgaga
     2101 acctacgcgc cgcggccgcc gtgctgacgg ccggcgggta ctcggtccga gtcgcgacgg
     2161 cgccggacgg gcgcgcgttg tgctgcggcc gcacctacct tgcgacgggc atgctcgacg
     2221 aggcgcgcgc agaagcgcgg cgcttgctcg acgcgctcgc gccgctcgcg gcgcgcggcg
     2281 tgccgatcgt gggactcgag ccgtcctgca ttctcgggct ccgcgacgag ctcaagatgc
     2341 tgtgccccgg aacggcgagc cgcgacgtcg cacgcgcggc tctgacgttc gaggaattcc
     2401 tcgtgcgaga gcgcgccgcg gggcgttggc agcgcgcgtt caaacgcgct ccgttcaagc
     2461 gcgcgctcgt gcacggccat tgccatcaaa aagcattcgg cgccatgccg gcagtggtgg
     2521 cagcgttgca actagtaccc gatctaacag tcgaggtcgt cgcatcgagt tgctgcggca
     2581 tggccggcgc gttcggctac gaagccgagc acctcgacgt gtcgctgcgc atggccgagg
     2641 cggcgttgct accggccgtg cgcaacgccg gcgacgatac cgtcgtcgtc gccgacggca
     2701 cgagttgccg ccatcagatc cacgacggcg cgcagcgcaa cgcggagcac gtcgcgcgcg
     2761 tgctggcgtc ggtactttaa cggttactcc gatccgctcg gttcggcccg cgagcgcttg
     2821 agccgcgaca gctgtaggcg acaccgtgtc atacgtcaca accacgccga cggctggtga
     2881 acttacggtt ccagtattgt gccggaaccg gaatgggtaa tcagaacact gcccttgcga
     2941 cgaccgctgc acccccgtcg acgtccctga ccgctcttgc cgagcaactt cgcgacggac
     3001 cgctgcacga cctgatccag ctacagctga aagccaccga gctcgcagac cgtctcgcgg
     3061 cgagccctgc tgatcgcatc gaggatctcg agcgcctggt ccggctctcg ttgactgcca
     3121 tggagcagtt tcatgccttt acgcgcgaat ttgcggcggt tcttcgggag ctcaccgatg
     3181 cccgccgcca cccgcactga gctatacacg cggctcgtgg agctctgcca agagttccga
     3241 gcacacggcg acctcgaatg cgaggtcgcc ttggacgagc accacaccaa gttcgacgag
     3301 gagctcagcg aagtcgtctt ccgaacggta cgggagctgc tgacgaacgt ccggcagcac
     3361 gccaaagcga cgcgagtcct ggtctcgagc atcgggcgcc gcgacggctc gatcggcatc
     3421 ctcgtcgccg acgacggcgt cggcctaccc gcgcaccggc gccgcggcaa cccgttcgac
     3481 gaaagcggcg gcatcggctt gtggagcatc gatcagcgct tgcgcgcctt cgacgcggtc
     3541 ctcgacgtcg aggctgccga cggcaagggc acgcgggcga tggtcattct gcccggcaag
     3601 ctgctcgtcg ccgattagcc gcccgagatc ggcacggcct gctcgcgcag ctctaggccg
     3661 gcggccttca aaccgctgac gagcacgtcg tagtagtcgc gatcgaagcg ccagcgctcg
     3721 aaattcgtca gcgcctccgc ctcgaactcg ggatacagct cgcgcagccg ccgcacggcc
     3781 tttgccgcga tttcgtcgcg gccgccatgc gccgcggccg ccgcgacgat ggcttgcgcc
     3841 atgacccagt tttgcgtgtc gaccttctgc gcgaacgaga gggcctcggc gtagcggtgc
     3901 tgccgcagcg aggtcacggc atacgccaag ttgtacagcg ccggcggcgt cttcgataaa
     3961 gccttggctc tctgcaggag cgcgagaccg cgctcggact cgccgcggac cgtcagcaag
     4021 aacccgcctt gcgcgagcgc ctgagcgttg ccggggcgca gcgcgatcgt gcgttcgatc
     4081 gtctgctgaa atgccgcgtc gccgtcgaag aattgcacgc gcgtcaacgc gagattgccg
     4141 aggaagtcgt cgggatcgag cagcagcgcc ttggccgtcg attcgcgcgc ggcggacagc
     4201 tcgttctcgc cgttgcgacc gaacgtggcc gcgtagttgt cgacgtagag catcgcgaga
     4261 ccggaccaca cctgcgcaac ctcgggctgc aaaacgctga cgttctgaaa gcacagcaag
     4321 gcgtcgcgat gcacctcggg cgctttgcgg cggcggtagt cgtagtaagt cacgaggcaa
     4381 tcgcgcagct tcggcgcctg cacggagcgg cgcgcccgcg cgagctccgc ttcgaagatc
     4441 gggccatacg gggcggcgat cgccgcgacg tcgcgcgcga cctgctcttg cagcaccggc
     4501 aactgctcga tctcgagcga ctcgtcgtag gccgcggtcc aaagctgcat tccgctgccg
     4561 gcctcgatga gccgcactgt gatgcgagcg tggtccgccg tgccccgtac gctgcccgtc
     4621 agcaggtagc cggtcgtccc cggggcatc
//
LOCUS       DATLEE010000526         7745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4311685, whole genome shotgun sequence.
ACCESSION   DATLEE010000526 DATLEE010000000
VERSION     DATLEE010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4311685"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VM692_11340"
     CDS             <1..642
                     /locus_tag="VM692_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ52807.1"
                     /translation="GASNPTYTLNDPGGWCKLITRDAVGGGRSRVEAPFFNTGVLETV
                     GVDAAVNWSKEFGPGEFGINTVATFLDKFETQDNPADKIFDAAGTLDQGGQYDYRLVT
                     TFNYTFGGGSSLGLQWRHYPSIKDDDAARDPTTRILPVPSYNVFNLFARHQISEKIEF
                     RGGIDNLLDEIPPIVGADPGTAALPRDDNLGDTNAGFYDVLGRRFFVGLQMNF"
     gene            complement(726..1787)
                     /locus_tag="VM692_11345"
     CDS             complement(726..1787)
                     /locus_tag="VM692_11345"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HUQ52808.1"
                     /translation="MANGLELAAQHDAAGRHEDAINALAVATKSGDIDAMTELSKRLI
                     VGDRAPYLPAEGASLLNDAARQGSSSAQLCLACLTALGAHVPQSWGAALGLLVEAAER
                     GSRSAADQLRLLARADDPPRSGASASDYRGLARAIDVDGWFAPCAGITLHDDPLVRQL
                     PDLLPPELCRWLIAKARGRLRPALIYSGESSNDIADEMRTNSVAVWNLGCMDLVNVVL
                     QYRIAAACGVPIANLEAPTALHYKVGEQITNHYDFLNPSSPSYRTEIATRGERVITFL
                     TYLNDDYAGGETDFPRIGVRHKGQRGCGLFFRNAHPTAGADARSLHAGRPPTSGEKWL
                     VSQFIRSRAVFNTQAENVG"
     gene            complement(1792..2967)
                     /locus_tag="VM692_11350"
     CDS             complement(1792..2967)
                     /locus_tag="VM692_11350"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HUQ52809.1"
                     /translation="MHLDRGELEEAEREYARAVALDPQDVAAQIGLAKLRYMCGDASF
                     ARDLVAAVAAHRSNGFLQVHFANMLRQIGDLAGAEVMLRDLIARQGPVPELQASLAAV
                     LHSAGRLEEAVVEARAAAEARVDDSAVILNFVAVLLSLGRADEAMPRIRAQRAKTPFH
                     PSWIAYEATAARLLDAPAYAELYDYARLVRVYDLPAPRGWSSTAEFNTALAADLAARH
                     TLRQQPFDQTLRFGTQTMGDLARDANPIMQTALAAFDEAIADYRASVGQAADHPLTAQ
                     NVGTARYVGCWSVRLRQNGFHVNHLHPEGWLSSAYYVAVPAESADPAQQSGWLKFGEP
                     ALPVPGAGPAYSVKPQPGRLVLFPSYMWHGTTAIRGTETRLTMAFDITTNPRPPGRQ"
     gene            3206..3571
                     /locus_tag="VM692_11355"
     CDS             3206..3571
                     /locus_tag="VM692_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52810.1"
                     /translation="MRYLALLVLVPLLGGCAGQDVVQPLGFESSSSATRVADTLVADT
                     PDAESLGDPAAGRICKTTKRPGSNIADTVCYTREEQLARQEASKDKVAELQREQRWRD
                     QVIQEAQMRNRYPAGLGPR"
     gene            complement(3544..4815)
                     /locus_tag="VM692_11360"
     CDS             complement(3544..4815)
                     /locus_tag="VM692_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52811.1"
                     /translation="MVSFEVRTVCIVACHASALVAATSWQALDAQPADADGNIIAQIE
                     SAQAEGGPYSADLLDPLKNLGFLYEERGEYQLAAAALERARQVVRANYGLTSLDQAPL
                     LEQQIRSEEALGRFREVWDLEQSLLSLAKAHPEDLRTVPILRDVGDRRMDVLTRYLAG
                     DRPPQIVLGCFYAPEPRARADGGRCDAGSRSVLIQSIVSDAQTSYLDAIRVMLRHELY
                     SSAELRELEMRLVASSYEHDRYDAGRQSLRRLLAYSLATPDPRSSQIEALVHLADWEL
                     LFELDQVAALESYARAFELLSQDDTTRASIERIFAPEIPAVLPVFAPNPLASTPSATS
                     AGYVDVAFDITRFGRSRNIEILSTSTISATATRESLVQLIARSRFRPRAVNGELARSA
                     PIVLRYYVDEPLPEATGPGEEPRQRGPKPAG"
     gene            complement(4932..>7745)
                     /locus_tag="VM692_11365"
     CDS             complement(4932..>7745)
                     /locus_tag="VM692_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HUQ52812.1"
                     /translation="IPGAQRPGDDPHAYLPPPAERGGTPESSEEHAVADAPEARPGVT
                     PRSASPKTTSSASSKVIEIRGAREHNLRNIDLKIPRDKLTVITGVSGSGKSTVAFDIL
                     FAEGQRRYLESLNAYARQFVEPAGRADVDAVYGIPPTVAIEQRTSRGGRKSTVATLTE
                     IYHFLRLLFVKLGVQHCPDCGVPIDAQTLDAIHAKVMKRYRGKRVALLAPLVIGRKGY
                     YTELAEWAAGRGYSALRVDGVSTPTDHWPRLDRFKEHDIELPVAELDVKPAAGAELAA
                     ALAQAVKLGRNMVRVRPLEVPEGGLPIDDSGKAAGELFSTERACPKCSRSFEPLDPRL
                     FSYNSRYGWCPECYGTGIELTGFDAEQSGEEDAWTEDGDADPAACASCGGRRLKPEAL
                     AVRFHGETIAELTARSIAASLASFDKLKLAPREREIARDVVAELLSRLSFLELVGLGY
                     LALDRAAPTLSGGEAQRIRLAAQLGSNLRGVCYILDEPTIGLHPRDNALLLDTIGKLA
                     AKGNTVVVVEHDEDTIRRAEHVVDLGPGAGRNGGEVVVNGSLHDLMQNERSITGRMLA
                     APPRHPLLERRKTRKNGGETAWLELAGVTRHNLRKLDVALPLARLVCVTGVSGSGKST
                     LIRDVLEDNLTRLVPLRGLPRGVTGRGHAPKLAHLASLQGWQGLGRVLEVDQTPIGKT
                     PRSCPATYVGIFDEIRKLYAQTTEARLRGYEASRFSFNAAGGRCEGCEGQGMKRIEMS
                     FLPDVRVNCERCAGKRFNAETLAVLFRDKSIGDVLAMTVDGAHAFFAAQPKIHHPLEL
                     LRDVGLGYLALGQPSPTLSGGEAQRVKLVTELAKVRPESLRSRPAAPTLYVLDEPTIG
                     LHIADVEKLVRVLHRLVDAGNSVIVIEHNLDVLAEADWIVDLGPEGGAGGGRVVAQGT
                     PEAVARGRSHTARALQAFLARRR"
BASE COUNT         1211 a         2587 c         2663 g         1284 t
ORIGIN      
        1 ggcgccagca acccgaccta cacgctcaac gatccgggcg gttggtgcaa gctgatcact
       61 cgcgacgccg tgggcggcgg gcgctcgcgc gtcgaggcgc cgttcttcaa cacgggcgtg
      121 ctcgagacgg tcggcgtcga cgcggccgtc aactggagca aggagtttgg gcccggcgag
      181 ttcggcatca acacggtcgc aactttcctc gataaattcg agacgcagga caacccggcc
      241 gacaaaatct tcgatgcggc cggcacgctg gaccaaggcg gccaatacga ttaccgcctc
      301 gtgacgacct tcaactacac gttcggcggc ggctcgagtc tcggcttgca gtggcgacac
      361 tatccgtcga tcaaggacga tgacgccgcg cgcgatccga cgacgcgcat tttgccggtg
      421 ccttcgtaca acgtcttcaa tctgttcgcc cggcaccaaa tcagcgagaa gatcgagttc
      481 cgcggcggta tcgacaacct gctcgacgag atcccgccga tcgtcggcgc ggatccgggc
      541 acggcagcgc taccgcgcga cgacaatctc ggcgatacca acgccgggtt ctacgacgtc
      601 ctggggcggc gcttcttcgt cggcttgcag atgaatttct agtacgttcg gacggtccac
      661 agcgaagggc gagcccactc gggctcgcct tttgtttttc tgcggcgtcg aacggcgcct
      721 tttgctcagc cgacgttctc ggcctgcgtg ttgaaaacgg ctcgactgcg aatgaactgg
      781 ctgacgagcc acttctcgcc gctcgtcggc ggccggccgg catgcagcga gcgggcgtcg
      841 gcaccggcgg tcggatgcgc gttgcggaag aacaggccgc agccgcgctg acccttgtgt
      901 cgaactccga tgcgcggaaa gtccgtctcg ccgccggcgt aatcgtcgtt gagatacgtc
      961 aggaacgtga tgacacgctc gccgcgcgtc gcaatctcgg tgcgatagct cgggctgctg
     1021 ggattcaaga agtcgtagtg attcgtgatc tgctcgccga ctttgtagtg cagcgccgtc
     1081 ggcgcttcga ggttggcgat cggcacgccg caggctgctg cgatgcgata ttgcagaacc
     1141 acgttgacga ggtccatgca gccgaggttc cagaccgcga cgctgttggt gcgcatttcg
     1201 tcggcgatgt cgttcgagct ttcgccgcta tagatgagcg ccgggcgcag ccggccgcgc
     1261 gctttggcga tcagccagcg gcagagctcg ggcggcagaa gatccggaag ctggcgcacg
     1321 agcggatcgt cgtgcagcgt gatgcccgcg catggcgcga accaaccgtc aacgtcgatc
     1381 gcgcgcgcga gcccgcgata atcgctggcg ctggctccgc tccgaggggg gtcgtccgcg
     1441 cgcgccaaca ggcgcagctg gtctgcggca cttcgcgagc cgcgttccgc ggcttcgacg
     1501 aggagaccga gcgctgcccc ccagctctga ggcacgtgtg ccccgagcgc ggtgaggcaa
     1561 gccaagcaca gctgtgcgct gctgctgcct tgtcgggcgg catcgttgag caagctcgcg
     1621 ccttcggcgg gcagataagg cgcccggtcg ccgacgatga ggcgcttgct gagctccgtc
     1681 atcgcgtcga tgtcgccgct tttcgtagcc accgcgagcg cgttgatggc gtcttcgtgg
     1741 cggcccgcag cgtcatgctg agccgcgagc tcgagtccgt tggccatact cttattggcg
     1801 cccaggtgga cgggggttcg tcgtgatgtc gaacgccatc gtaaggcgcg tctcggtgcc
     1861 gcggatggcg gtcgttccgt gccacatata agaaggaaag agcaccaacc gacccggctg
     1921 cggtttgacg ctgtaggcgg gcccggcacc gggtacgggc agcgccggct caccgaactt
     1981 cagccagccc gattgttggg ccggatcggc ggattcggct gggacggcaa cgtagtacgc
     2041 agagctcagc cagccttccg gatgaagatg attgacgtgg aagccgtttt gccgcaaccg
     2101 cacggaccaa cagccgacgt agcgcgcggt gccgacgttt tgcgccgtca gaggatgatc
     2161 ggcggcctgg ccgacgctgg cgcgatagtc ggcgatcgcc tcgtcgaacg ccgcgagcgc
     2221 cgtttgcatg atggggttgg cgtcgcgggc cagatcgccc atcgtttgcg tcccgaagcg
     2281 cagggtttga tcgaacggct gctgccgcag cgtgtgacgc gcagcgaggt cagcggctag
     2341 cgccgtgttg aactcggccg tgctcgacca accgcgcggc gccggcaagt cgtagacgcg
     2401 tacgagccgc gcatagtcgt acagctcggc gtaggccggc gcgtcgagca ggcgtgccgc
     2461 agtcgcctcg taggcgatcc aggaaggatg gaaaggtgtc ttggctcgct gcgcgcgaat
     2521 gcgcggcatc gcttcatcgg cgcggccgag ggacagcagc acggcgacga agttgagtat
     2581 tacggccgag tcgtcgacgc gcgcttcggc cgcggctcga gcttcgacga ccgcctcttc
     2641 gagtcgcccg gcggaatgca gcacggccgc gagcgacgct tggagctcgg gaacgggccc
     2701 ttggcgtgcg atgaggtcgc gcaacatgac ctctgcgccg gcgagatcgc cgatctgccg
     2761 caacatgttg gcgaagtgca cctgcaaaaa gccgttgctg cgatgggcgg cgacggccgc
     2821 gacgaggtcg cgcgcgaacg acgcatcgcc gcacatgtaa cgcagcttcg ctaggccgat
     2881 ctgcgcagcc acgtcttgcg ggtcgagggc gactgctcgc gcgtactcgc gctccgcctc
     2941 ctcgagctcg ccgcggtcga gatgcaaagc cgctcggtgc gccggcgtcg ctccggcgtt
     3001 ctctgccgct cccgcacgcc cttttgctgg ttggctagcc attcgtcgtt cgcttcagag
     3061 gagcgatttc aacatgggcg gcgaaaatgc gtccaccccc gaacccctgg gctcgctccg
     3121 cgaggcgaaa accgtgcggt gcggtaggtg ccgagcgggc ggctgtgcta gcgtttcgcc
     3181 tgagacgcac cgctcggagg ttcccatgag gtatctcgcc ctactcgtgt tggtgccgct
     3241 gctcggcggc tgtgcgggtc aagacgtcgt gcagccgctc ggcttcgagt cgagcagctc
     3301 cgccacgcgg gtcgccgaca cgctggtcgc cgacacgccg gacgcggagt cgctcggaga
     3361 tccggccgcc gggcggatct gcaagacgac gaaacgcccc gggtccaaca ttgccgacac
     3421 cgtctgctac acgcgcgagg aacagcttgc gcggcaggag gcgagcaaag acaaggtcgc
     3481 ggaactgcag cgcgagcaac gttggcgcga ccaggtcatc caggaagcgc agatgcggaa
     3541 ccgttacccc gcgggcttgg gcccacgctg acgcggctcc tcgccggggc ccgtcgcttc
     3601 cggcaacggt tcgtcgacgt aatagcgcag cacgatcggc gcgctgcgcg cgagctcgcc
     3661 gttcaccgct cgcgggcgga atcgactgcg cgcaatcaac tgcaccaagc tctcgcgcgt
     3721 cgcagtggcc gagatcgtcg aggtcgaaag aatctcgatg ttgcggctcc tcccgaaccg
     3781 tgtgatgtcg aacgccacat cgacgtagcc ggcggacgtc gcgctcggcg tcgaggccag
     3841 cgggttcggc gcaaaaacgg gcagcaccgc cggaatctcc ggggcgaaga tgcgctcgat
     3901 cgaggcacgc gtcgtgtcgt cttggctcag gagctcgaag gctcgcgcgt acgactcgag
     3961 cgccgctact tggtcgagct cgaagagcaa ctcccagtcg gcgagatgca cgagggcctc
     4021 gatctggctc gagcgcggat ccggggtcgc caggctatac gccagtaagc ggcgcagact
     4081 ctgacggccg gcgtcgtaac ggtcgtgctc gtagctgctt gcgacgagcc gcatctccag
     4141 ctcgcggagc tcggcgctcg aatacagctc gtgccgcagc atgacgcgga tggcgtcgag
     4201 gtaactcgtc tgcgcatcgg agacgatcga ctgaatcagc acgctgcggc tgcccgcgtc
     4261 gcagcgcccg ccgtccgcgc gggcgcgcgg ctccggtgcg tagaagcagc ccagcacgat
     4321 ctgtggcggg cgatcgccgg caagatacct cgtcagcacg tccatgcgcc ggtcgccgac
     4381 gtcgcgcagg ataggcacgg tgcgtagatc ctcggggtgc gccttcgcga gactgagtag
     4441 gctctgctcg aggtcccata cctcgcggaa tcggccgagc gcttcttcgc tgcgaatctg
     4501 ctgctcgagc agcggcgctt gatcgagcga agtcagacca tagttggcgc gcaccacttg
     4561 gcgggcacgc tcgagcgcgg cggcggcgag ctggtactcg ccgcgttcct catacaggaa
     4621 tcccaggttc ttgaggggat cgagcagatc ggccgaatac gggccgccct ccgcctgcgc
     4681 gctctcgatc tgcgcgatga tattgccgtc ggcgtccgcc ggttgcgcgt ccaacgcctg
     4741 ccacgaagtg gcggcgacga gcgccgaggc gtgacacgcg acgatgcaga cggtccgcac
     4801 ttcgaatgag accaccgggc ccctccgagc acacctgtca tcggccgtcc gctcgggcca
     4861 cccggcggtt cggcctccga tggaagtcta gcactcgctc gagtgtggac gcggcgcgtc
     4921 ccgcgggtca gctaacggcg ccgcgcgaga aacgcttgca gcgcacgagc cgtgtgcgaa
     4981 cggccgcgtg cgacggcctc cggcgtcccc tgcgccacga cgcggccgcc gccggcgccg
     5041 ccttcgggcc cgaggtcgac gatccaatcc gcttcggcca gcacgtcgag gttgtgctcg
     5101 atgacgatga cgctgttgcc ggcgtcgacg agccggtgca gcacgcgcac gagcttctcg
     5161 acgtcggcga tgtgcagccc gatcgtcggc tcgtcgagca cgtacagggt cggcgccgcc
     5221 gggcgcgagc gcaggctctc cgggcgcact tttgcgagct ccgtcacgag cttcacgcgc
     5281 tgcgcctcgc cgccggacaa cgtcgggctc ggctggccga gcgcgagata gcccaagccg
     5341 acgtcgcgta ggagctcgag cgggtgatgg atcttcggct gagccgcgaa gaacgcgtgc
     5401 gctccgtcga ccgtcatcgc gagcacgtcg ccgatgctct tgtcgcgaaa cagcacggcc
     5461 agcgtctcgg cgttgaagcg cttaccggcg cagcgctcgc aattgacgcg cacgtcgggc
     5521 aggaagctca tctcgatgcg cttcatgccc tgcccttcgc agccttcgca gcggcctccc
     5581 gccgcgttga acgagaagcg gcttgcctcg tagccgcgca gccgcgcttc agtcgtctgc
     5641 gcgtacagct tgcggatctc gtcgaagatg ccgacgtagg tcgcggggca cgagcgcggc
     5701 gtcttgccga tcggcgtctg atcgacctcg agcacgcggc cgaggccctg ccaaccctgc
     5761 aagctcgcta gatgcgcgag cttcggcgcg tgaccgcgcc cggtcacgcc ccttggtagt
     5821 ccccgaaggg ggacgagccg cgtcagattg tcctcgagca cgtcgcggat caacgtgctc
     5881 ttgccgctgc cgctgacgcc cgtcacgcag acgagccgcg ccagcggcag cgcgacgtcg
     5941 agcttgcgca gattgtggcg cgtgactccc gcgagctcga gccatgccgt ctcgccgcca
     6001 ttcttgcgcg tcttgcgccg ctcgagcagc ggatggcgcg gcggcgcggc cagcatgcgc
     6061 cccgtgatcg agcgctcgtt ctgcatgagg tcgtgcagtg agccgttgac gacgacctca
     6121 ccgccgttgc gcccggcgcc cgggccgaga tcgacgacat gctcggcgcg acgaatcgtg
     6181 tcctcgtcgt gctcgacgac gacgaccgta ttgcccttcg ccgcgagctt gccgatcgtg
     6241 tcgagcagca gcgcgttgtc gcgcggatgc aagccgatcg tcggctcgtc gaggatgtag
     6301 cagacgccgc gcaggttcga gccgagctgc gcggcgagcc tgatgcgttg cgcttcgccg
     6361 cccgacagcg tcggcgctgc gcggtccagc gcgaggtagc ccaggccgac gagctccaga
     6421 aagctcagcc ggctcaggag ctccgcgacg acgtcgcggg cgatctcgcg ctcgcgcggc
     6481 gcgagcttga gcttgtcgaa cgacgctagc gacgccgcga tcgagcgcgc cgtcagctcg
     6541 gcgatcgtct cgccgtggaa gcgcacggcc agcgcctcgg gcttgagccg ccgccccccg
     6601 catgacgcgc atgccgccgg gtccgcgtcg ccgtcctcgg tccaggcgtc ttcctcgccg
     6661 ctctgctcgg cgtcgaagcc cgtcagctcg atgccggtgc cgtagcactc ggggcaccaa
     6721 ccgtaacggg agttgtacga gaacagccgc gggtcgagcg gctcgaagct acggctgcac
     6781 ttcgggcacg cgcgttccgt cgagaacagc tcgccggccg ctttcccgga atcgtcgatc
     6841 ggcagccccc cttcggggac ttccaagggt cgcacgcgca ccatgttgcg gccgagcttg
     6901 acggcttggg ccagcgccgc cgcgagctct gcgccggcgg ccggcttcac gtcgagctcg
     6961 gcgacgggca gctcgatgtc gtgctccttg aaccgatcga ggcgcggcca gtggtcggta
     7021 ggcgtcgaga caccgtcgac gcgcaacgcg gaatagccgc gacctgccgc ccactcggcg
     7081 agctcggtgt aataaccctt gcggccgatc acgagcggcg ccagcaacgc cacgcgtttg
     7141 ccgcgatagc gcttcatgac cttcgcgtga atcgcgtcga gggtctgcgc gtcgatcggc
     7201 acgccgcaat cggggcagtg ctgcacgccg agcttgacga acagcaggcg caggaaatga
     7261 taaatctcgg tcagcgtcgc gacggtgctc ttgcggccgc cgcggctcgt gcgctgctcg
     7321 atcgcaacgg tcggcggaat gccgtagacg gcgtcgacgt cggcgcggcc ggcaggctcg
     7381 acgaactgcc gcgcgtacgc attcagcgac tcgagatagc ggcgttggcc ttcggcgaac
     7441 aagatgtcga acgcgacggt gctcttgccg ctgccgctga cgcccgtgat gaccgtcagt
     7501 ttgtcgcgcg gaatcttcag atcgatgttg cgcaagttgt gctctcttgc gcctcttatc
     7561 tcgatcacct tagaagaggc gctcgaggtg gtcttgggcg aggccgagcg cggcgtcacg
     7621 cccggcctcg cttcaggcgc atcggccacc gcgtgctctt cactcgattc gggtgtgcca
     7681 ccgcgctctg cgggcggagg caggtatgcg tgagggtcat cgccggggcg ctgcgcgcca
     7741 gggat
//
LOCUS       DATLEE010000527         2291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6262925, whole genome shotgun sequence.
ACCESSION   DATLEE010000527 DATLEE010000000
VERSION     DATLEE010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6262925"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VM692_11370"
     CDS             complement(<1..759)
                     /locus_tag="VM692_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ52813.1"
                     /translation="MSTRTILSFCGTALALLATHAHAQFGSEPAKLEVIKVRDDIYVI
                     SNVAVPGLTTALITNDGVLLVDDKFEIDHASIMAALKTVTSEPVKYVINTHYHGDHSG
                     GNAKLQALGTLAVASIEARASMVKANQSGQPNITVGDRAEIIIGGKRVEIHKVGRAHT
                     DGDVVVLFPEQRVLASGDIFANGPGTSAQLVDYPGGGSAKLWPLAVDQALELQFDTVI
                     PGHGLVSTKADLTAYRARAQRFADTLQQLVSQGKS"
     gene            985..2145
                     /locus_tag="VM692_11375"
     CDS             985..2145
                     /locus_tag="VM692_11375"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011646121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HUQ52814.1"
                     /translation="MRPLEGLRILALEVWGAGPYGSQLLAALGAEVIKIENPATGGDP
                     ARAGGPHFFGERESLYFQAWNTSKRSVALDLKTDSGRGQFAELVRTTDAVVNNLRGDQ
                     PAKLGLDYATLGELNPAIVCAHISAYGRDNERTSWPGYDFLMQAEAGLMSLTGEPDGP
                     PARFGPSIIDFMTGTTAMVGLLSCLWNAQRTGKGCDVDVSLFDVALHQLNYSATWYLN
                     AGHAPTRLERSSHFAVAPVQTFPTADGWVFVMCMSDKFWLELAAALELRELARDPRFA
                     SGNKRHEHRRELTTALDPAFRKRTTAEWLRRLSGVLPIGPVYGVAEALDSPFVAATGM
                     IQTVPHRGKQDLRVLTSPIKIDGERAAPAPAPELGEGNDELLGHGKHGAKQP"
     gene            2142..>2291
                     /locus_tag="VM692_11380"
     CDS             2142..>2291
                     /locus_tag="VM692_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HUQ52815.1"
                     /translation="MKLRGLRVVDLSQFLPGPYLTLALADHGAEVIKIEPPDGDPGRK
                     IGVADG"
BASE COUNT          387 a          744 c          779 g          381 t
ORIGIN      
        1 cgacttgccc tggctcacga gttgctgcag cgtgtccgcg aagcgctggg cgcgagcgcg
       61 ataggccgtg agatcggctt tcgtcgagac caggccgtgg cccggaatga cggtgtcgaa
      121 ctgcaactcg agggcttgat cgacggcgag cggccagagc ttggcgctgc cgccgcccgg
      181 ataatcgacg agctgtgccg acgtgccggg cccgttcgcg aagatgtcgc ccgacgcgag
      241 cacgcgctgc tcggggaaca agacgacgac gtcgccatcc gtatgagcgc gcccgacctt
      301 gtggatctcg acgcgcttgc cgccgatgat gatttcggcg cggtcgccga cggtgatgtt
      361 gggttgcccc gattgattcg ccttgaccat gctcgcgcgc gcttctatcg acgcgacggc
      421 cagtgtgccg agcgcctgga gcttcgcatt accgccgctg tgatcgccgt ggtagtgcgt
      481 gttgatcacg tacttcacgg gctcactggt gaccgtcttc agcgcggcca tgatgctggc
      541 gtgatcgatc tcgaacttgt cgtcgacgag cagcacgccg tcgttcgtga tcaacgccgt
      601 cgtcaggccc ggcacggcga cgttgctgat gacgtagatg tcgtcgcgga ccttgatgac
      661 ctcgagcttc gcgggctcgc tgccaaactg cgcgtgcgca tgggtcgcga gcagcgccaa
      721 agcggtgccg cagaagctca aaatcgttct cgtgctcatg agtggtcgag tctccgaaga
      781 aaaaggtcgg gttgcggctc gcagtttagt cgaagagcga agatgagtcc cgccggcagc
      841 ccgtcttcgc gcggcgcccg ccggcaggct tgtcaagaca acatcgagga acttagccta
      901 cgtccgcgcg cgtatagctg aggagataga agagttgtac ggacagatca accggttgca
      961 tcgggcgcag cattgctggc tcgcatgagg ccgctcgaag ggctccgaat actggcgctc
     1021 gaggtctggg gcgccggacc ctacggctcg cagctgctcg ccgcgctcgg cgcggaagtc
     1081 atcaagatcg agaaccccgc gacgggcggc gatccagcgc gggcgggcgg cccacatttc
     1141 ttcggcgagc gtgagagcct gtactttcag gcttggaaca cgagcaagcg cagcgtcgcg
     1201 ctcgatctga agaccgacag cgggcgcggt caattcgcgg agctcgtccg gacgaccgac
     1261 gcggtcgtca acaacttgcg cggcgaccag ccggcgaagc tcgggctcga ctacgcgacg
     1321 ctcggcgagc tgaatcctgc gatcgtctgc gcgcacatct cggcgtacgg ccgcgacaac
     1381 gagcgcacga gctggcccgg ctacgatttc ctgatgcaag cagaagcggg cctcatgagc
     1441 ttgaccggtg agccggacgg cccgcccgcg cggttcgggc cgtcgatcat cgatttcatg
     1501 acgggcacga cggccatggt cggtttgctg tcgtgcttgt ggaacgccca gcgcaccggc
     1561 aaaggctgcg acgtcgatgt ctcgctgttc gacgtcgcgc tgcaccagct caactactcg
     1621 gcgacgtggt acctgaacgc aggccatgcg ccgacgcggc tcgagcgcag cagtcacttc
     1681 gcagtcgcgc cggtgcagac gttcccgacg gccgacggct gggttttcgt catgtgcatg
     1741 agcgacaagt tctggctcga gctcgccgcc gcgctcgagc tccgggagct cgcacgcgat
     1801 ccgcgcttcg ccagcggcaa caagcggcac gagcatcgac gcgagctaac gaccgccctc
     1861 gatcccgcgt tccgcaaacg cacgaccgcc gagtggctca ggcggctctc gggtgtgctg
     1921 ccgatcggcc ccgtctacgg cgtcgcggag gcgctcgata gcccgttcgt cgcggctacg
     1981 ggcatgattc agaccgtgcc gcaccgcggg aagcaggatc tgcgcgtgct gacgagcccg
     2041 atcaagatcg acggcgagcg agcggcgccg gcgcccgcgc ccgagctcgg ggagggcaac
     2101 gacgagctgc tcggccacgg caagcacgga gccaagcaac catgaagctc cgcgggctgc
     2161 gcgtcgtcga tctgtcgcaa tttctgccag gcccgtatct gacgctcgcg ctggccgacc
     2221 acggcgccga ggtcatcaag atcgagccgc ccgacgggga ccccggccgt aagatcggtg
     2281 tcgccgacgg g
//
LOCUS       DATLEE010000528         6731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9549532, whole genome shotgun sequence.
ACCESSION   DATLEE010000528 DATLEE010000000
VERSION     DATLEE010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9549532"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VM692_11385"
     CDS             complement(<1..461)
                     /locus_tag="VM692_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HUQ52816.1"
                     /translation="MATPKEVRFSDDARQRMLKGVNVLANAVKVTLGPKGRNVVLEKS
                     FGAPTVTKDGVSVAKEIELEDKFENMGVQMVKEVASQTSDAAGDGTTTATVLAQAILR
                     EGLKSVSAGFNPMDLKRGIDKAAIAITEELKKLSQPCKDDKSIAQVGTISA"
     gene            complement(507..797)
                     /gene="groES"
                     /locus_tag="VM692_11390"
     CDS             complement(507..797)
                     /gene="groES"
                     /locus_tag="VM692_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HUQ52817.1"
                     /translation="MKLRPLHDRVVIRRTEEERKSPGGIVIPDTATEKPSKGEVLSVG
                     KGKILENGEVRPLDLKKGDRVLFGKYSGTEVKIDGEDLLVMREDDVMAVIEK"
     gene            989..1321
                     /gene="cutA"
                     /locus_tag="VM692_11395"
     CDS             989..1321
                     /gene="cutA"
                     /locus_tag="VM692_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HUQ52818.1"
                     /translation="MSEETMVVLTTCGNDDDAQALARVLVERRLAACVNAISKVASTY
                     RWKGEVQQDQETLLIIKTTAPRLAALERAIREHSKYEVPELLALNVEAGGADYLAWVR
                     ESVAELKD"
     gene            1329..3542
                     /gene="dsbD"
                     /locus_tag="VM692_11400"
     CDS             1329..3542
                     /gene="dsbD"
                     /locus_tag="VM692_11400"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HUQ52819.1"
                     /translation="MSSLQFAARLLTACFALSAGSAVLAQQDQPPPAEEVFLYKASAD
                     AERVYLDFDIRDGYYAYRTRFGFDSGTNGVALGAARFPNGEIHSDEFFGEQQVYRHQF
                     QVAIPYRRSAARESFELKLELQGCADFGLCYLPQEWTANVTLPPPAFLGAGSIDTTAT
                     GDLLPVDDAFTMNARFDKPNELTVAWQIAPGYYLYRDKLTTTVNGPIELGAANLPKGV
                     AHTDDNFGDVEVFFDYVEVKVPFARASPDAIDVELTAGFQGCKEGSICYPPGEQLMSL
                     VLPATSEFPAPAAAGGDELVSEQDQWAARIVNGTWPELLGWFYLGGLLLSFTPCVLPM
                     VPILSSIIAGQGTVSTSRGFALSVSYVLGMAFTYTILGAASALAGQQLQAALQEPWIL
                     SAFAGVFVVLALGLFGAFQFQMPTTIQTRLSNLANQQKAGTFAGTAVIGALTSLIMTT
                     CVAPVLVGALVVIGQTGDVTRGAGALFAMSIGMGSPLLLVGASAGYLLPKVGPWMDTV
                     KAGFGVIMLGFAIYLLGRFLPPTPTLFLWAVLVFFTGVFIGAFDTLPPSPAAWRRVAK
                     GIGVLVCVYGILLFIGASLGGGNLLQPIPRAALTSGSTGGALASLDDEEALEFRSIAT
                     VAQLDAALAEARAANRPVMLDFTAEWCISCKEMEEYTFPDSGVIGALEPFLLLRADVT
                     DNDDDDKALLQRFKSFGPPTIAFFDRSGFERSNFKLVGFVPAEKFSEHVQSLAAL"
     gene            3581..4192
                     /locus_tag="VM692_11405"
     CDS             3581..4192
                     /locus_tag="VM692_11405"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HUQ52820.1"
                     /translation="MARSRPVLLTVALSAAAVLGFVAYRTFMPTPATEGADEGPATSA
                     ALTVETLPDFSLGNLAGEQQSIQSWPGKPLLINFWATWCGPCLREIPMLKELQTARPD
                     LQIVGIAIDKRDLVVGFAANTQFNYPILIGQAEAWAAAAALGVNIYALPFTVFTAADG
                     SILGVHTGELHAEHLEAFRTIADDLKAGTISVAEARGRIAAGI"
     gene            4283..4732
                     /gene="aroQ"
                     /locus_tag="VM692_11410"
     CDS             4283..4732
                     /gene="aroQ"
                     /locus_tag="VM692_11410"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HUQ52821.1"
                     /translation="MARLLLLNGPNLGQLGRREPGIYGTATLADIESLLRTEATAAGH
                     DFDAFQSDAEAELIRRIHAARDQGVAVILFNPAAFTHTSVALRDALLAAQIPFIELHL
                     SNVAAREAFRHHSYFADIALGTIAGFGVTSYELALTAAIRHLKKGGK"
     gene            4732..5193
                     /gene="accB"
                     /locus_tag="VM692_11415"
     CDS             4732..5193
                     /gene="accB"
                     /locus_tag="VM692_11415"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015848145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HUQ52822.1"
                     /translation="MDIRKVKKLIELLEESGISEIEISEGEESVRISRYPKPGAITMT
                     QAPAAAPAAPAPATAPAAAPTAADAAPPAIRGQQVTAPMVGTFYTGPAPGSKPFVEVG
                     TEVKPGDTLCVIEAMKMMNQIESEYAGRVVSVLVENGSPVEFGQPLFVIEE"
     gene            5229..6572
                     /gene="accC"
                     /locus_tag="VM692_11420"
     CDS             5229..6572
                     /gene="accC"
                     /locus_tag="VM692_11420"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HUQ52823.1"
                     /translation="MLDKVLIANRGEIALRIFRACRELGIKTVAVHSTADTTLKHVLL
                     ADETVCIGPPRSQQSYLNMPAIIAAAEVTDAVAIHPGYGFLSENADFAERVEESGFIF
                     IGPKPETIRLMGDKVSAIKTMRAAGVPCVPGSDGPLGDDPEVNARLAHEIGYPVIIKA
                     AAGGGGRGMRVVHSDAALFNAVSITRAEALSAFGSDQVYMEKFLEHPRHVEFQVLADS
                     HGNAVHLGERDCSMQRRHQKVIEESPAPGISEEERRKMGQRCVEACVKIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELVTGIDIVKTQLAIAAGERLPFSQEDIRWRGHA
                     IECRVNAEDPKTFVPSPGTVSLWHPPGGPGIRVDSHMYSGYAVPPYYDSLIAKVIAHG
                     EHRRAAIARMTTALQEIVVDGISTNIPLHQEIFQHGAFKAGGTDIHYLERRLGLK"
BASE COUNT         1189 a         2190 c         2120 g         1232 t
ORIGIN      
        1 ttggccgaga tcgtgccgac ttgcgcgatc gacttgtcgt ccttgcacgg ctgcgacagc
       61 ttcttgagct cttcggtaat cgcaatggcc gctttgtcga taccgcgctt caggtccatg
      121 gggttgaacc cggccgacac cgacttcaag ccctcgcgca ggatcgcttg cgcgagcacc
      181 gtggccgtcg tcgtgccgtc gccggcggcg tcggaagtct gcgaagcgac ttctttgacc
      241 atctgcacgc ccatgttctc gaacttgtcc tcgagctcga tctctttcgc gaccgagacg
      301 ccgtctttcg tgacggtcgg ggcgccgaag ctcttctcga gcacgacgtt gcggcctttc
      361 gggcccagcg tgactttgac ggcgtttgcg agaacattga cgcccttcag catgcgctga
      421 cgagcatcat cggaaaaacg aacttctttg ggagtcgcca tgactctgtc tcctggaatg
      481 gtttgaacgc ttgagtggta gccgggttac ttttcgatga cggccatgac gtcgtcttcg
      541 cgcatgacga gcaggtcttc gccgtcgatc ttgacctcgg tacccgaata tttgccgaag
      601 aggacgcggt cgcccttctt caggtcgagg gggcggactt cgccgttctc gagaatcttg
      661 cctttaccga ccgagagcac ttcgcccttg ctcggctttt cggtggccgt atcggggatc
      721 acgatcccgc cgggggactt gcgctcttcc tccgtgcgcc ggatcacaac gcgatcatgt
      781 aggggacgaa gtttcataaa tgctctccct tgtgtaagca cttgattttc ggatggattc
      841 ccgaccggcg cgattttgtt agcactcggt caaagtgagt gctaatcata tggatcgaaa
      901 agcggattgc aaggcctgag agccgagtca tgacattggt tgaaaccggc cgttatgctg
      961 gcggccgtcc cactgacggt aggccgacat gagcgaagag acgatggtcg tcttgacgac
     1021 atgcggcaac gacgacgacg cgcaagcatt ggcgcgcgtg ctcgtcgagc gacggctcgc
     1081 cgcctgcgtc aacgcgatca gcaaggtcgc ttcgacgtac cgctggaagg gcgaggtcca
     1141 acaggatcag gagactctgt tgatcatcaa gacgaccgcg ccgcggctcg ctgcgctcga
     1201 acgcgcgatt cgcgagcatt cgaagtacga ggtgcccgag ctgctcgcct tgaatgtcga
     1261 ggccggtggc gccgactatt tagcctgggt gcgggaatcg gtcgccgagt tgaaggacta
     1321 acgctgtcat gagctcactg cagttcgccg ctcgcctgtt gaccgcgtgc ttcgccttga
     1381 gcgccggcag cgccgtgctc gcgcaacagg accagccgcc gccggccgag gaagtcttcc
     1441 tttataaggc gagcgccgac gccgagcgcg tctacctcga cttcgacatc cgcgacggct
     1501 actacgcgta ccggacccgc ttcggcttcg acagcgggac gaacggcgtc gcgctcggcg
     1561 cggctcgttt tccgaacggc gaaatccaca gcgacgagtt cttcggcgag cagcaggtct
     1621 atcgacatca gttccaagta gcgatcccgt atcgccgcag cgcggcacgc gaaagcttcg
     1681 agctcaagct cgagctacaa ggttgcgccg acttcggcct gtgctatctg ccgcaggagt
     1741 ggacggcgaa cgtgactctg ccgccgccgg ctttcctcgg cgccggctcc atcgacacca
     1801 cggccacggg cgatctgctg cccgtcgacg atgccttcac gatgaatgcg cgcttcgaca
     1861 agccgaacga gctcaccgtc gcctggcaga tcgcgccggg ctactacctc taccgcgaca
     1921 agcttacgac gaccgtcaac ggccccatcg agctgggggc tgccaacttg ccgaagggcg
     1981 tcgcgcacac ggacgacaat ttcggcgacg tcgaagtgtt cttcgattac gtcgaggtca
     2041 aagtgccgtt cgcgcgcgca agccccgacg cgatcgacgt cgagctgacg gcgggcttcc
     2101 agggctgcaa agaaggcagc atctgttatc cgccgggtga gcagctgatg tcgctcgtgt
     2161 tgcccgcgac cagcgagttt cccgcgcccg ctgcagccgg cggcgatgag ctcgtctccg
     2221 agcaggatca atgggcggcc cggatcgtga acgggacgtg gccggagctc ctcggctggt
     2281 tctacctggg cggtctgttg ctctcgttca caccgtgcgt gttgccgatg gtgccgatct
     2341 tgtcgagcat catcgccggt caaggcaccg tctcgacgag ccgcggattc gcgctgtcag
     2401 tgagctatgt cctcggcatg gccttcacgt acacgatcct cggtgcggcg tcggctttgg
     2461 cagggcagca gctgcaggct gcattacagg aaccgtggat cctttcggcg ttcgcgggcg
     2521 tattcgtcgt gctggcgctc ggcctgttcg gcgcattcca gttccagatg cccaccacaa
     2581 ttcagactcg gctgtcgaac ctcgccaatc agcagaaggc ggggaccttc gcgggcaccg
     2641 cagtcatcgg cgctctgaca tcgctgatca tgacgacctg cgtcgccccg gtgctcgtgg
     2701 gagctctcgt cgtcatcggc caaacggggg acgtcacgcg cggcgccggt gctctgttcg
     2761 cgatgagcat cggaatggga tcgccgctgc ttctcgtcgg cgcctcggcc ggctacctct
     2821 tgccgaaggt cggcccgtgg atggacaccg tcaaagccgg attcggcgtg atcatgctcg
     2881 gcttcgcgat ctacttgctc ggacgcttcc tgccgccgac gcccacactg ttcctctggg
     2941 ccgtgctcgt gttcttcacc ggcgtcttca tcggcgcgtt cgacacgctt ccgccgagcc
     3001 cagccgcgtg gcgccgcgtg gccaaaggca tcggcgtatt ggtgtgcgtt tacgggatcc
     3061 tgctgttcat cggcgcgtcc ttgggcggtg ggaacctgct gcaacccatc ccgcgggcgg
     3121 ccctgacgtc cggctcgacg ggcggcgcgc tcgcctcgct ggacgacgag gaagcgctcg
     3181 aatttcgctc gatcgcaacc gtggcgcagc tcgatgccgc gctcgccgaa gcgcgggccg
     3241 cgaaccgacc cgtgatgcta gacttcacgg cggagtggtg tatttcctgc aaggaaatgg
     3301 aggaatacac gttcccggac tcgggggtca tcggcgcgct cgaaccgttc ttgctgttgc
     3361 gcgccgacgt aacggacaac gacgacgatg acaaagcctt gctacaacgc ttcaagagct
     3421 tcggtccacc gacgatcgcc ttcttcgatc gcagcggttt cgagcgcagc aacttcaagc
     3481 tcgtcggctt cgtgccggcc gaaaagttca gcgagcacgt gcaaagtctc gccgcgcttt
     3541 agggagtctt ccttcgggga ttaccaaggg gcgcccctcc atggctaggt cgcggccggt
     3601 gctgctgacg gtggcgctga gcgcggctgc cgttctcggg ttcgtcgcct atcgcacctt
     3661 catgccgacg cctgcgaccg agggcgccga cgaggggccg gcgacgtccg ccgcgctcac
     3721 cgtcgaaaca ttgccggatt tctcgttggg caacctcgcc ggcgaacagc aatcgatcca
     3781 aagctggccc ggtaagccgt tgctcatcaa cttctgggcg acgtggtgcg ggccgtgcct
     3841 gcgcgaaatc ccgatgctaa aagagctgca aaccgcgcgg cccgatctac aaatcgtcgg
     3901 catcgctatc gacaagcgcg acctagtggt cgggttcgca gcgaacacgc aattcaacta
     3961 tccgatcctg atcgggcaag ccgaggcctg ggccgctgcc gccgcgctcg gcgtcaacat
     4021 ctacgcgctg ccgttcacgg tttttacggc tgccgacggc agcatcctgg gggtccacac
     4081 gggcgagttg cacgccgagc acctcgaggc gtttcgcacc atcgcggacg acctcaaggc
     4141 cggcacaatc agcgtcgccg aggcccgcgg tcggatcgca gccggcatct agagccgttc
     4201 tagccctaaa acgcggcata gtggcgccaa ctgcgccgaa aaacgggtaa actcgcgcga
     4261 tccaatttga aggttcgcgc ggatggcccg tctcctgctg ctcaacggcc cgaacttggg
     4321 gcagctcggc cggcgtgagc caggcatcta cggtacggcc acgctggccg acatcgagtc
     4381 gctgctgcgt acggaagcca cggcagccgg ccacgatttc gatgccttcc agagcgacgc
     4441 cgaggccgag ctgatccgac gcattcatgc cgctcgcgac caaggcgtcg ccgtcattct
     4501 gttcaatccc gccgccttca cgcacacgag cgtcgcgctg cgcgacgcgc tgctggcggc
     4561 gcagattccg ttcatcgagc tgcatctatc gaacgtcgcg gcacgcgagg cgttccgcca
     4621 ccactcgtat ttcgccgata tcgcactcgg gacgatcgcc ggcttcgggg tcacgagcta
     4681 cgagctcgcg ttgacggccg cgattcgcca cctgaagaaa ggggggaagt gatggacata
     4741 cgcaaagtaa aaaaactcat cgagctactc gaggaatcgg ggatttccga aatcgagatc
     4801 tccgaaggcg aggagtccgt tcgcatcagc cgttacccga agccaggtgc gatcacgatg
     4861 actcaggccc ctgcggccgc ccccgcggcc ccggctccag caaccgcacc cgccgccgcc
     4921 ccgaccgccg cagacgccgc cccgccggcc atacgcggtc agcaagtcac cgcgccgatg
     4981 gtcggtacgt tttatacggg cccggcgccg ggttcgaagc ccttcgtgga ggtcggcaca
     5041 gaagtgaagc ccggtgacac gctctgcgtc atcgaagcca tgaagatgat gaatcagatc
     5101 gagtcggagt acgccggccg cgtcgtcagc gtcctcgtcg agaacggcag cccggtcgag
     5161 ttcggccaac cgctgttcgt aatcgaagag tagtcaacga aaagtaaccg caagcggcgc
     5221 aaaaaacgat gctggacaaa gttctgatcg ccaaccgcgg cgagatcgca cttcgcatct
     5281 ttcgcgcttg ccgcgagctc ggtatcaaaa ccgtcgccgt gcactcgacg gccgacacca
     5341 cgctgaagca cgtcctgctc gccgacgaga cggtgtgcat cggcccaccg cgatcgcagc
     5401 agagctacct gaacatgccg gccatcatcg ccgcggcaga agttaccgat gccgtggcca
     5461 tccatcccgg ctacggcttc ctgtccgaga atgcggactt tgccgagcgc gtcgaggaaa
     5521 gcggattcat cttcatcggc cctaagcccg agacgatccg gctcatgggc gacaaggtct
     5581 ccgcgatcaa gacgatgcgg gcggccggtg tgccgtgcgt ccccggttcc gatgggccgc
     5641 tcggcgacga tccggaggtc aacgcgcgac ttgcccacga gatcggttac cccgtcatca
     5701 tcaaagcggc cgccggcggc ggcggacgcg gcatgcgggt cgtgcacagc gacgctgcgc
     5761 tgttcaacgc cgtgtcgatc acgcgcgccg aagcgttgtc ggccttcggc agcgaccagg
     5821 tctacatgga aaagtttctc gagcatccgc gccacgtcga gttccaggtg ctggccgata
     5881 gccacggcaa cgccgtgcac ttgggcgaac gcgattgctc gatgcagcgg cggcatcaga
     5941 aagtcatcga ggaatcgccg gcgccgggga tctcggagga agagcgccgg aagatgggcc
     6001 agcgctgcgt cgaggcctgc gtaaagattg gctaccgcgg cgcgggcacg ttcgagtttc
     6061 tgtacgagga cggcgagttc ttcttcatcg agatgaacac gcgcgtgcag gtcgagcatc
     6121 ccgtcacgga gctcgtgacg ggcatcgaca tcgtgaagac gcagctcgcg atcgcggccg
     6181 gcgagcggct gccgttctcg caggaagaca tccggtggcg cggccatgcg atcgagtgcc
     6241 gcgtcaacgc cgaggatccg aaaacgttcg tgccgtcgcc cggcaccgtg agcctttggc
     6301 atccgccggg cggccccggc attcgcgtcg acagccacat gtacagcggc tatgcggtgc
     6361 cgccctacta cgattcgttg atcgcgaagg tcatcgcgca cggcgagcac cgccgcgccg
     6421 cgatcgcgcg catgacgacg gcgctgcaag aaatcgtcgt cgacggcatc agcacgaaca
     6481 ttccgctgca ccaagagatc ttccagcacg gcgcgttcaa ggccggcggc acggacattc
     6541 actacctcga gcggcgactc ggtctgaagt aacggccgcc gtggtttggc aacgcttgac
     6601 gctccaaatc gcggcagccg acgtgccgag ggccgaggca ttgctgacgc tagccggagc
     6661 ggcagcgatc gcgctgcgcg acgcggccga ccagcccgtc ttcgagcccg agccgagcac
     6721 agcgccgctc t
//
LOCUS       DATLEE010000529         8436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7015328, whole genome shotgun sequence.
ACCESSION   DATLEE010000529 DATLEE010000000
VERSION     DATLEE010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7015328"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VM692_11425"
     CDS             complement(<1..502)
                     /locus_tag="VM692_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52824.1"
                     /translation="MSAVDAVDTQRAKSTQRPATSSVTTDFAWRVLILLNLFRLSVGA
                     VFLAVYYLVDEPRIVGETDATLAWRALIGLLVAGCVFLVLLQRRSPGTRPQTYLQFAA
                     DLIAVALLIHASGGISSGLGGVLIVSVGTLALLLPTERAFLLAAVSTLALLGQQTYAQ
                     LQGITTG"
     gene            614..1779
                     /gene="sucC"
                     /locus_tag="VM692_11430"
                     /pseudo
     CDS             614..1779
                     /gene="sucC"
                     /locus_tag="VM692_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779632.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
     gene            1779..2654
                     /gene="sucD"
                     /locus_tag="VM692_11435"
     CDS             1779..2654
                     /gene="sucD"
                     /locus_tag="VM692_11435"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HUQ52825.1"
                     /translation="MSILVDKNTKVICQGLTGKQGTFHTQQCLDYGTKVVAGVTPGRG
                     GEKHLNLPVFDTVAEAKKATGATVSMIYVPAPFAADAILEAADAGLELVVCITEGVPV
                     LDMVKVKAVLKSTNVRLIGPNCPGIITPGACKIGIMPGSIHKPGSIGIVSRSGTLTYE
                     AVNQTTLRGLGQSTCVGIGGDPVRGMNFIDCLALFEKDPATTGIIMVGEIGGSDEEAA
                     AEYIAKHVTKPVVGYIAGVTAPPGKRMGHAGAIISGGKGTAKEKFAALEKAGVRTVRS
                     LADLGEAMANELPRR"
     gene            2656..4260
                     /locus_tag="VM692_11440"
     CDS             2656..4260
                     /locus_tag="VM692_11440"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HUQ52826.1"
                     /translation="MKIGLAQINTRVGAIDANTRKVLDYSAAAREQGCDLVLFPELTL
                     CGYPPEDLLLHRGLRSRVEAAFARVRDEVRGIAVYLGYPEYTGDAIYNSAALLRDGEL
                     LANHRKVALPNYAVFDEKRYFQPGDGATVASLSGSKFGLIICEDVWLAAPCRAPAEAG
                     AEMILVINGSPFHRAQQQARERVLALRAQENSLPLIYVNMVGGQDELVFDGGSVVVDA
                     KGDVRFRAPLFEEGLHVVELERRAGRLEPKPATVTPVPPLAERVYRALVLGTRDYVEK
                     NGFAGVILGLSGGVDSALTLTIAVDALGADRVHAVMMPSRFTSQMSRDDAELQAKHLG
                     VHYDVLSIESMFETTLAVLQDVFAGLPPDTTEENIQARCRGVLLMAISNKTGKMLLTT
                     GNKSEMAVGYATLYGDMAGGFAPLKDCTKTLVYQLASYRNGLSQVIPGRVITRPPTAE
                     LRDNQKDSDSLPPYDVLDAILEAFIEKDYSVDQITALGFDRATVVRVLQMVKRAEYKR
                     RQAPPGVRVSSRAFGRDWRYPITSGY"
     gene            4332..6167
                     /locus_tag="VM692_11445"
     CDS             4332..6167
                     /locus_tag="VM692_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52827.1"
                     /translation="MSVPIVSSGQESASSERAWRLRSLWDVAAWAVASLIAMFLARLV
                     RDDAALGDLYLPSGNDSFYHARRILDAAFGQGLRQFDARLHAPDGDWVAWPWAYDYLL
                     AKATQLAVWLQPSLDPMAFLTYVPVAWIAVNAALLLAVCRAIRLSPEMRLIAMLSFAI
                     SPLTQLLHAAGMIDHHYVEHTFVLLAIWLGSRWFQQPGDRRRALALGIALGIAPGFHN
                     GLFILQLLPLAGVLALWLRGTAPSGPALRAFAVALVVTTQVVLLPSEPYRRFMFEFGL
                     LSWFHFYVALCTAAAMVYMGWQRLSMRSFAGLAALCAVLAAPLIGQVLVGAGFIAGDF
                     SILSDIREAQSPLEIMRTFGAREVLGYYSWMLVLVPLLLAYYVVQAFRETTPDRIYYA
                     LAAVCGLAMLLSQQRLQYFGFFAFVTSGLLIVDALRRQHRWHRGVVFAAVLALTVLAY
                     QPALRERLFVVYAPGADSEYASAFAIFLDLQSQCAADPGVVLASPDDGNAILFHSACS
                     VIANNFILRPEDAEHIGEVQRLMRLSPAEIRRERPDVKYVFVRAADFSILADGVARLA
                     TEIPIAKELLGDQQPPAGYYIVKTVRWSLGDEDRIYARLYKISGE"
     gene            6177..7214
                     /locus_tag="VM692_11450"
     CDS             6177..7214
                     /locus_tag="VM692_11450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ52828.1"
                     /translation="MASTIDIKTSAGRSVGSRDSGEVLPTALEGLHAPRIAVLIPCYN
                     EAASIAEVVGDFREALPDATIYVYDNNSTDGTRGRAAAAGAVVRGEPLQGKGNVIRRM
                     FADNEAEGYVLVDGDGTYAAGSAAAMVDQLLKNSLDMVNGARVAVTGAAYRRGHAFGN
                     KLLTATVAAIFGSRIRDMLSGYRVMSRRFVKSFPALATGFETETELTVHALELRLPVA
                     EVDTPYRSRSEGSDSKLRTYRDGTRILWTILKLAKAERPLQFFGVVAAALALSATVLA
                     WPLLTVYLETGLVPRLPTAVLVTGMMLLAFVSLVTGLILDAITHGRAEAKRLYYLSLQ
                     GPTVRERRRTP"
     gene            complement(7252..7992)
                     /locus_tag="VM692_11455"
     CDS             complement(7252..7992)
                     /locus_tag="VM692_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HUQ52829.1"
                     /translation="MLLGVILAALAGCGRENVVEDQGPELLYQKGYQAMEASNFAGAI
                     QYFNALEARYPFSPETRQAQLDLIYVYYRSLQPEQAIDAAEEFERENPTHERIDYCLY
                     MRGLAYFDQAPNIIERLMRVDLSLRPPKDTMKSFSTFQELIRRFPDSEYVPDARQRMV
                     FLRNRLAEYENHVADYYVRRGAYVAAVNRAKYALEHYPEAPQLEKTLQILVAAYEQLG
                     MADLAADARRVLRQSFGDDAATAQLLKP"
     gene            8124..>8436
                     /locus_tag="VM692_11460"
     CDS             8124..>8436
                     /locus_tag="VM692_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HUQ52830.1"
                     /translation="MSRIVRRLAVPPDLAGRRLDQAAAALLPEFSRSRLRSWIDAGEL
                     TVGGRAAKARLKLKGGEELALATELEAAVEPKPEPIPLVVLHADEALLVIDKPVGLVV
                     HP"
BASE COUNT         1462 a         2654 c         2816 g         1504 t
ORIGIN      
        1 cgcccgtggt gattccttgc agctgcgcgt aggtttgctg cccgagcaac gccagcgtgg
       61 agacggcggc gagcaggaag gcgcgctcgg tgggcaacag cagcgccagt gtgccgaccg
      121 agacgatgag gacgccgccg aggccgctcg agatgccgcc gctcgcatgg atcaggaggg
      181 ctacggcgat gagatcggcc gcgaattgca gatacgtctg cgggcgcgtg cctggagatc
      241 gtcgctgcag cagcacgagg aaaacgcagc ccgcgacgag caggccgatc agcgcgcgcc
      301 atgccagcgt cgcgtcggtt tcgccgacga tgcgcggctc gtctacgagg taatacacgg
      361 ccagaaacac ggcgccaacg gacaggcgga acaaattgag aaggatcaga actcgccagg
      421 cgaagtcggt cgtcaccgaa ctggttgcag ggcgctgcgt ggatttggcg cgttgcgtgt
      481 cgacggcatc gacggcggac atggcgcgga attctacctg atcggcgcgc gccgaccggg
      541 cgccgtgctt tgcggggttc gagggtttcg tggagaatac cgccgccccg ccagttgctg
      601 aagacgcttt cccatgaatc tgcacgagta tcaatcgaag gtattattcg ccgagtacgg
      661 catccccgtg ccgaaaggcc aggttgccac gacgccgcag gaggcgaagg cggcggccac
      721 cgcgctgggc ggcaagctgt gggtcgtgaa ggcgcaggtc catgcgggcg gccgtggtaa
      781 ggccggcggc gtcaggctcg ccaagtcggt ggccgaagtc gagcagtacg ccaaagcgat
      841 gctcggcacc cggctcgtga ctcatcagag cggccccgaa gggctgcccg tcgattacgt
      901 ctacgtcgag acgggatcgt ccatcgagcg agagctctac ttgagcttgg tcgtggatcg
      961 cagctccgag cgcatcgcgg taatggcctc ggccgccggc ggcatggaca tcgaagaggt
     1021 cgcgcacaag acgcctgaga agattctcac cctgacgctg catccggccg ccggcttcga
     1081 ggactaccaa acgcgtgagc tcggcttcgg tctcggtttg agcgccgagc agcaaggcga
     1141 gctgcgcgac atcctgcgca agctgttcaa gctattccgc gagtgcgacg cgagcctcgt
     1201 cgagatcaac ccgctgatcg tgacgaagga cggccacgtc gtcgcgctcg acgcgaagat
     1261 caacatcgag gacaacgcgc tgttccgcca aaagcggctc tcagggatgc gcgacaaggc
     1321 gcaggaggac gagcgcgagc gcgtggcgca cgagaacgac ctcaactacg tctcgctcga
     1381 cggcaacatc gcgtgcatgg tcaacggcgc gggcctcgcg atggcgacga tggacttgat
     1441 caagctgcac ggcggcgagc cggcgaattt cctcgacgtc ggcggcggcg cgacggccga
     1501 gcgcgtgacg caggctttca agatcatcct gacgaacaag aacgtcaaag cgatcctcgt
     1561 gaacattttc ggcggcatcg tgcgttgcga cacgatcgcc gaaggcgtca tcacggcggt
     1621 caagcaagtg cacgtcggcg tgcccgtcgt cgtgcgcctc gagggaacga acgtcgagag
     1681 ggacgcgaga tgctcgccgc cagcggcctc gaggtcgtgg cggccaccgg cctcaccgat
     1741 gcggcgtcga aagtcgtgca attggccgga gccgcctgat gagcattttg gtcgacaaga
     1801 ataccaaggt catttgccaa gggctgacgg gtaagcaagg tacgtttcac acgcagcagt
     1861 gcctcgacta cggcacgaag gtcgtcgccg gtgtcactcc gggtcgcggc ggcgagaagc
     1921 atttgaatct gccggtcttc gacacggtcg ctgaagcgaa gaaggcgacg ggcgcaaccg
     1981 tcagcatgat ctacgtgcca gcgccgttcg cggccgatgc gatcctcgag gcggccgatg
     2041 ccggtctcga gctcgtcgtc tgcatcaccg aaggcgtgcc agtgctcgac atggtcaagg
     2101 tcaaagctgt gctgaagagc acgaacgtgc gcctgatcgg tccgaactgc cccggcatca
     2161 tcacgccggg cgcctgcaag atcggcatca tgccgggctc gattcacaag ccgggctcga
     2221 tcggcatcgt gtcgcggtcc ggcacgttga cgtacgaggc cgtgaaccag acgacgcttc
     2281 gcggcttggg ccagagcacc tgcgtcggca tcggcggcga ccccgtgcgc ggcatgaact
     2341 tcatcgactg cctcgcgctg ttcgagaagg accccgcgac gacgggcatc attatggtcg
     2401 gcgagatcgg cggctccgac gaggaagccg cggccgagta catcgccaag cacgtgacga
     2461 agcccgtcgt cggctacatt gcaggcgtta cggcgccgcc cggcaaacgc atgggccacg
     2521 caggcgcgat catctcgggc ggcaagggca cggccaagga gaaattcgcg gcgctcgaga
     2581 aggccggcgt gcgcaccgtg cgctcgctcg cggacctcgg cgaggccatg gctaacgagt
     2641 tgccacggcg ttgaagtgaa gatcgggctc gcccagatca acacgcgcgt cggcgccatc
     2701 gacgcgaata cccgcaaagt tctcgactac tccgccgccg cgcgcgagca gggctgcgat
     2761 ctcgtgttgt ttccggagct gacgctctgc ggctatccac ccgaggactt gctgctgcat
     2821 cgtggcttgc gcagccgcgt cgaggcggcg ttcgcgcgag tccgcgacga ggttcgcggc
     2881 atcgcggtct acttgggcta ccccgagtac accggcgatg cgatctacaa cagcgcggcg
     2941 ctgttgcgcg acggcgagtt attggccaac caccgcaaag tcgcgctgcc gaactacgcc
     3001 gtgttcgacg agaagcgcta cttccagccg ggcgacggcg cgaccgttgc gagcttgagc
     3061 ggcagcaagt tcggcttgat catctgcgag gacgtctggc tggcggcgcc gtgccgtgcg
     3121 ccggccgaag caggagccga gatgatcctc gtcatcaacg gctcgccgtt ccaccgggcg
     3181 caacagcaag cgcgagagcg agtgctcgcg ctgcgcgcgc aagaaaactc gctgccgttg
     3241 atctacgtca acatggtcgg cgggcaggac gagctcgtgt tcgacggcgg ctccgtggtc
     3301 gtcgacgcca aaggcgacgt gcgatttcga gcgccgctgt tcgaggaagg cttgcacgtc
     3361 gtcgagctcg agcgccgcgc cggccgcctc gagccgaagc cggcgacggt cacgcccgtc
     3421 ccgccgctgg ccgagcgcgt gtatcgcgcg ctcgtgctcg gcacgcgcga ctacgtggag
     3481 aagaacggct ttgcgggcgt gatcctcggc ctctcgggcg gtgtcgactc ggcgttgacg
     3541 ctgacgatcg ccgtcgatgc gctcggagcg gaccgagtgc acgccgtcat gatgccgtcg
     3601 cggttcacgt cgcagatgag ccgcgatgac gccgagttgc aggccaaaca cctcggcgtg
     3661 cactacgacg tgctgtcgat cgagtcgatg ttcgagacga cgctcgcggt gttgcaagac
     3721 gtgttcgccg ggttgccgcc cgacaccact gaggaaaaca tccaggcgcg ttgccgcggc
     3781 gtgctgctga tggcgatctc taacaagacg ggcaagatgc tgctgacgac gggcaataaa
     3841 agcgagatgg ccgtcggcta tgccacgctc tacggcgaca tggcgggcgg cttcgcaccg
     3901 ctgaaagact gcacgaagac gctcgtctat cagctggcga gctatcgcaa cggcctctcg
     3961 caggtcattc cgggccgcgt catcactcgc ccgccgaccg cggagctgcg cgacaatcag
     4021 aaagacagcg actcgctgcc gccctacgac gtgctcgacg cgattctcga ggccttcatc
     4081 gagaaggatt actcggtcga ccaaatcacg gcgctcggct tcgaccgagc cacggtcgtg
     4141 cgcgtgctac agatggtgaa acgcgcggaa tacaaacgcc ggcaggcgcc gccgggcgtg
     4201 cgcgtcagca gccgcgcctt cggccgcgac tggcgctacc caatcacgtc cgggtattga
     4261 gccgacggaa cctgggtgga agcgggcgtg gatcacgccg acagttcgcc ccgactgcta
     4321 gtgtccaagg gatgagcgtg cccatcgtga gctccggcca agaatcggcg tcttccgagc
     4381 gtgcctggcg cctccgctcg ctgtgggatg tagcagcgtg ggctgtcgcg tcgttgatcg
     4441 ccatgtttct tgcgcggctc gtacgcgacg atgcggctct cggcgacttg tatctgccga
     4501 gcggtaacga cagcttctat catgcgcgtc gcattctcga cgccgcgttt gggcagggtc
     4561 tgcgtcaatt cgacgcgcga ctccatgcac ccgacggcga ctgggtcgcg tggccttggg
     4621 cctacgacta cctgctcgcg aaagccacgc agctcgctgt gtggctccag ccgtcgctcg
     4681 acccgatggc cttcctcacc tacgtgcccg tagcttggat cgcagtcaac gcggcgctgt
     4741 tactggccgt gtgccgggca atccggctgt cccccgagat gcggctgatc gcgatgctca
     4801 gcttcgcaat ctcgccgttg acgcagctgt tgcatgcggc gggcatgatc gatcaccact
     4861 acgtcgagca cacgttcgtg ttgctcgcga tctggctcgg cagccgctgg ttccagcagc
     4921 ccggcgatcg tcgccgcgcg ctcgcgttgg ggatcgcgct cggtatcgcg cccggttttc
     4981 acaacggcct cttcattctc caattgctgc cgctagcggg cgtgctcgct ttgtggttgc
     5041 gcggtacggc gcccagcggg ccggcgcttc gagcgtttgc cgtcgcgctc gtcgttacga
     5101 cacaggtcgt cctgctgccc tccgagccgt accgcaggtt catgttcgag ttcggcttgc
     5161 tctcgtggtt tcatttctac gttgcgctct gcacagcggc cgcaatggtg tacatgggct
     5221 ggcaacgact gtcgatgcgc agcttcgcgg gccttgccgc gctctgtgcc gtgctggccg
     5281 caccgctgat cgggcaagtg ctcgtcggcg ccgggttcat cgcgggcgac ttctcgattc
     5341 tcagcgacat ccgcgaagca cagagcccac tcgaaatcat gcgaaccttt ggtgctcgcg
     5401 aggtgcttgg ctattacagc tggatgctcg tgttggttcc gctgctgctt gcctattacg
     5461 tcgtgcaagc gttccgcgaa acaacgccgg acagaatcta ttacgcgctt gctgccgtct
     5521 gcggcttggc aatgctgctg agccaacagc ggctccaata cttcggcttt tttgcgttcg
     5581 taacttcggg tttgctgatc gtcgacgcgc tgcggaggca acaccgctgg catcgcggcg
     5641 tggtgttcgc agcggtgttg gcccttactg tcctggccta ccagcctgcg ctgcgcgagc
     5701 gattgttcgt cgtatatgct cccggagcgg attccgagta cgcaagcgca ttcgccatct
     5761 tcctcgatct ccagtcgcaa tgtgctgcag acccaggagt cgtgctcgca agtcccgacg
     5821 acggcaacgc gatactgttc cacagcgcct gctcggtgat cgcgaacaat tttattctgc
     5881 ggcccgagga tgcggagcac atcggcgagg ttcagcgcct gatgcgtctc tcgccggcgg
     5941 agatccgccg cgaacgaccc gacgtcaaat acgtgttcgt tcgcgcggcg gactttagta
     6001 ttctcgccga cggcgtggcg cggttggcaa ccgagattcc aattgcgaag gagttgctcg
     6061 gcgaccagca gccgcccgcg gggtattaca tcgtcaagac agtacgttgg tcgctcggcg
     6121 atgaagaccg catttacgcc cgcctctata agatctcagg cgagtagcga acggcgatgg
     6181 cttcgacgat cgacatcaaa acctcagcgg gccgatcggt tggctcgcgc gattccggcg
     6241 aggtgctgcc gaccgcgctc gagggcttgc acgcaccacg catcgccgtg ctgattccct
     6301 gctacaacga agcggctagc atcgccgagg tcgtcggcga ctttcgcgag gccttgcccg
     6361 acgcgactat ttatgtgtac gacaacaact cgaccgatgg cactcgcggg cgcgcggccg
     6421 cggcgggagc ggtcgtgcgc ggcgaaccgc tgcagggcaa aggcaacgtc attcgcagga
     6481 tgtttgccga caatgaagcc gaggggtacg tgcttgtcga cggcgacggt acctacgctg
     6541 cgggctccgc agcggcgatg gtcgaccaat tgctcaaaaa ttcgctcgac atggtcaacg
     6601 gtgctcgcgt cgcggttacg ggcgcggcct atcgccgcgg gcacgccttc gggaacaagc
     6661 tgctgacggc aaccgttgca gcaatcttcg gcagccgcat acgcgacatg ttgtcgggtt
     6721 atcgcgtgat gtcgcggcgg ttcgtgaagt cgttcccggc gttggcgaca ggtttcgaga
     6781 cggagaccga gcttacggta cacgctctcg agcttcgttt gcctgtcgcg gaggtcgaca
     6841 cgccgtatcg aagccggtcc gaaggttcgg acagcaagct gcggacgtac cgcgacggaa
     6901 cgcgcatctt gtggacgatt ctcaagctcg ccaaagcgga gcggccgctg cagttcttcg
     6961 gcgtcgtggc cgccgcgctc gcgctctcgg caacggtgct cgcgtggcct ttactgaccg
     7021 tctatctgga gacgggcctg gtgccgcggc tgccgacggc ggtgctcgtc acgggaatga
     7081 tgctgctcgc attcgtaagc ctcgtaacgg ggctcatcct cgatgccatc acgcatggcc
     7141 gggccgaggc gaaacggctg tattacttgt cgttgcaagg gccgacggtc cgcgagcgcc
     7201 ggcgcacgcc gtagcctgat cgagcagcgc gctccagcgc ggcaggacta gtcagggctt
     7261 gaggagctgg gccgtcgcgg cgtcgtcgcc gaagctctgt cgcagcacgc gccgcgcgtc
     7321 ggccgcgaga tccgccatgc cgagctgctc gtaggcggcg accaagatct gcagcgtttt
     7381 ctcgagctgc ggcgcctcgg ggtagtgctc gagcgcgtac ttggcacgat tgacggcagc
     7441 gacgtatgcg ccgcgccgta cgtagtagtc ggccacatgg ttctcgtact cggccagacg
     7501 gttgcgcagg aaaaccatgc gctggcgcgc gtcgggcacg tactcgctgt ccgggaaccg
     7561 gcgaatcagc tcctggaacg tcgagaacga cttcatcgtg tccttcggcg gacgcaacga
     7621 caggtcgacg cgcatcaggc gctcgatgat gttcggcgcc tgatcgaaat aggcgaggcc
     7681 gcgcatataa aggcagtaat cgatgcgctc gtgcgtcgga ttctcacgct cgaattcctc
     7741 ggcggcatcg atcgcctgct cgggttgcag gctgcggtaa tagacataga tgagatcgag
     7801 ctgcgcctgg cgcgtctcgg ggctgaacgg atagcgggct tcgagcgcat tgaaatattg
     7861 aatggcgccc gcgaaattag acgcttccat cgcctggtag cccttttggt acaggagctc
     7921 ggggccttga tcctcgacga cgttctcgcg cccgcagccc gcgagggcag ccaagataac
     7981 gcccaacagc aagacacttt tgccgactcg acgcacgtgc tggtccaaaa tgggtggtgg
     8041 cgggcaataa tgccgcgggt tttcgttggc tagttcgacg atgcagtata gcggaaagtt
     8101 tccatggccc taggacgggc cccgtgagcc ggatcgtgag gcgcctggcc gtgcccccgg
     8161 atctcgcggg ccgccgtctc gatcaggccg cggcggccct gctacccgag ttctcacgga
     8221 gccgattgcg gagctggatc gatgccggcg agctgacggt cggcggtcgt gcggccaaag
     8281 cccgcctcaa gctcaaaggc ggagaggagc tggcgctcgc gacggagctc gaggcggccg
     8341 tcgagcccaa gcccgagccg attccgctcg tcgtgctgca cgccgacgag gcgctgctcg
     8401 tcatcgataa acccgtgggt ctggtcgtcc acccgg
//
LOCUS       DATLEE010000530         5858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3300551, whole genome shotgun sequence.
ACCESSION   DATLEE010000530 DATLEE010000000
VERSION     DATLEE010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3300551"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VM692_11465"
     CDS             <1..783
                     /locus_tag="VM692_11465"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ52831.1"
                     /translation="EAYYLLGLAHYQLRDHLKALPAARRAVELAPDAPASWLELLTAV
                     LKQLGDHRAAIPWLEKLVELAPNNKTYWTELSLAYEQVGDYENSLATMRLAHGAELLT
                     DDADYRRLSDLLVHQGVPVAAAEVLEQGIAQRIVQPDEAAYTKVGTAWFMAGEPDKAV
                     PPLENAARAAGSGDAYVRLANVHITRQDWPAAIAALNAGLGRGQLTDEAHADLLMGVA
                     LYAEGKYAEAKTYLTTAAESERHRTIARSYLDAIAARTARAP"
     gene            complement(819..1514)
                     /locus_tag="VM692_11470"
     CDS             complement(819..1514)
                     /locus_tag="VM692_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ52832.1"
                     /translation="MRKETAVVVGVGPGLGTALVRTFANDGYTVYAAARSASALPGLS
                     EPGRVVTIDCDATDEADVESVLGAASAAGSLAVAVFNAGAFVRGSILDTDPEEFERCW
                     RVGCFAGFLLGRAAARRMLERGAGTILFTGATASLRGGASFVNLASPKFGLRAVAQSM
                     ARELGPKGIHVAHVIIDGQIHSERYAHLQAERGPDSLLEPDAIAAQYLALHRQPRSAW
                     TQELDLRPWSEKF"
     gene            complement(1520..1858)
                     /gene="trxA"
                     /locus_tag="VM692_11475"
     CDS             complement(1520..1858)
                     /gene="trxA"
                     /locus_tag="VM692_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009716492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HUQ52833.1"
                     /translation="MENPKTALLSVGEGDFERLVLDSAQPAVVDFSAAWCGPCRSIAP
                     VIEGLANDYADRVTVATVDIDDNPRLAAKYGVRSVPTVMLFAGGDVKRVLVGARSAQE
                     YRSSVDELLH"
     gene            complement(1872..3266)
                     /locus_tag="VM692_11480"
     CDS             complement(1872..3266)
                     /locus_tag="VM692_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUQ52834.1"
                     /translation="MRTTSVIAVTALLAACAVGPDYRAPETAATPFVATPEGVAQQPF
                     EAAWWQQFRDPSLDSLIERSLASDLDLAVATARVQEARAYLGTARRDRWPGASAELGR
                     SEGHQQQPGFTTSRTEVESYDLGIAALWELDLFGRVRRGVEAAGADADAAVAGLRDAQ
                     VLVAAEVARNYVELRGAQKRLGVARANLGYQQETLDLTRVRLELGRGTELDVASAAAR
                     LAATEATIPPLEAAETIAANRLAVLLGERPGALAAELAPREIAPHLTTIAVDSPAALL
                     QRRPDIRAAERELAAATARIGVAKADLFPRLTLSGFIGFVAGDADELGESTSRAWSLS
                     PVLSWAGFERGTYSNVVVAEARTAAALATYELTVLRALEETENAFVTYGSHRQRLEAV
                     VEQAAASRNAAELARVQYREGAVDFLRLLDAERTLLQAEDEVAAAETDLNSSVVLIYK
                     ALGGGWEAAPVPTI"
     gene            complement(3285..>5858)
                     /locus_tag="VM692_11485"
     CDS             complement(3285..>5858)
                     /locus_tag="VM692_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUQ52835.1"
                     /translation="IRTQNVQVAAGQLGSPPASSAAQFQLLVDTQGRLVTEEEFESIV
                     IKTGERGQITRLKDVARVELGASTYALRSLLDNEPAVAIPVRQQPGSSNAIDISNKVR
                     AKMAELKQDFPEGVDYRIVYDPTVFVRDSIAAVVQTLVEALLLVVLVVVLFLRTWRAS
                     VIPLAAVPVSLVGTLAAMHLLGFSLNALSLFGLVLAIGIVVDDAIVVVENVERNIAKG
                     LRPIEAAKQAMSEVTSPIIAISAVLIAVFVPTAFITGLSGQFYEQFALTIAISTVISA
                     INSLTLSPALAALLLKPHGEAPDRFQRGIDRAFGWAIGPFNRFFEWLSVHYVGLTGRT
                     LKRAGGAAVIYVALVGVAWLGFKSVPEGFIPQQDKEYLVAFAQLPEAATLDRTEDVIR
                     RMTDIMLAEPGVAHSVAFPGLSVNGFVNAPNVGIAFVALDDWEDPSFKKTRTANEVAA
                     ALNQKFAAIQDAYIAIFQPPPIQGLGTIGGFKLQIEDRAGLGYQALYEETQKIVGAGW
                     QTPGLTGLFSSYQVNVPQVRADVDRDKAMAQGVALNEIFDTMQVYLGSLYVNDFNRFG
                     RTYRVNAQADMPFRLEPEDIGQLKVRNAQGGMVPLGSFVALEQAAGPDRVMHYNGYPS
                     AEINGGPAPGFSSGEAEALIAGLAKASLPNGMHVEWTELAYQKQLAGNAGLWVFPISV
                     VLVFLVLAALYESLTLPLVVILIVPMTLLSAIAGVWLVGGDNNIFTQIGLIVLVGLAA
                     KNSILIVEFARERELAGASTWDAIIDAARLRLRPILMTSIAFTAGVVPLVLAHGAGAE
                     MRHAMGVAVFAGMIGVTIFGLVLTPVFYWLVQRLGTGARSRAERRSINDMNAAAAALI
                     P"
BASE COUNT          990 a         2053 c         1954 g          861 t
ORIGIN      
        1 gaggcctact acctgctcgg cctcgcgcat taccagctca gggaccactt gaaggcgctg
       61 ccagccgcgc gccgcgccgt ggagctcgcg ccggatgcac cggctagctg gctcgagctg
      121 ctgacggccg tgctgaagca actcggcgat catcgcgccg cgattccatg gctcgagaag
      181 ctcgtcgagc tcgcgcccaa caataagacg tattggacgg agctgtcgct cgcgtacgag
      241 caggtcggcg actacgaaaa ctcgctcgcg acgatgcggc tcgcgcacgg cgccgagctg
      301 ctgacggacg atgccgacta tcggcgactg tcggacttgc tcgtgcacca gggcgtgccc
      361 gtcgcggcgg ccgaagtgct cgagcagggg atcgcgcagc gcatcgtgca gccggacgag
      421 gctgcgtaca cgaaggtcgg cacggcgtgg ttcatggccg gcgagcccga caaagccgtg
      481 ccgccgctcg agaacgccgc ccgcgccgcc ggcagcggcg atgcctacgt gcggctcgcg
      541 aacgtgcaca tcacgcggca ggattggccc gctgcaatcg ccgcactgaa cgcgggcctc
      601 ggccgcggcc agctgaccga tgaagcgcac gccgatctgc tgatgggcgt cgcgctgtat
      661 gccgagggca agtacgccga agcgaagacg tatctgacga cggccgccga atccgagcgg
      721 catcgcacga tcgcgcgcag ttacctcgac gcgatcgccg cgcgcacggc gcgcgcgccc
      781 taggcgtcac tcggtcgagc cgcccgcgct cgacgccgtt aaaacttctc gctccacggc
      841 cgcaagtcga gctcctgcgt ccacgcgcta cgcggctgcc gatgcagcgc gagatattgc
      901 gccgcgatcg cgtcgggctc gagcagcgag tccggcccgc gctcggcttg caagtgcgcg
      961 tagcgctccg aatgaatctg gccgtcgatg atgacgtgcg cgacgtgaat gcctttcggg
     1021 cccagctcgc gagccatgct ttgcgccacc gcgcgcaaac cgaacttcgg cgacgcgaga
     1081 ttgacgaagc tggcgccgcc gcgcagcgac gccgtggcgc ccgtgaagag gatcgtgccg
     1141 gcaccgcgct cgagcatgcg gcgcgcggcc gcacgtccta gcaagaatcc cgcgaagcag
     1201 ccgacgcgcc agcagcgctc gaactcctcg gggtccgtgt cgagaatgct gccgcgaacg
     1261 aatgcgccgg cgttgaagac cgcgactgcc agtgagcctg ccgccgaggc cgcgccgagc
     1321 acactctcga cgtcggcttc gtcagtggca tcgcagtcga tcgtcacgac gcgcccgggc
     1381 tcgctcaagc caggcagcgc tgaggcggaa cgcgcggcgg catagaccgt gtagccatcg
     1441 ttcgcgaacg tgcgcacgag cgcggtgccg agccccggcc cgacgccgac cacgaccgcg
     1501 gtttccttgc gcatcgccgt caatgtagca gctcgtcgac gctcgagcgg tactcctgcg
     1561 ccgagcgcgc accgacgagc acgcgcttga cgtcgccgcc ggcgaacagc atcacggtcg
     1621 gcacggagcg cacgccgtac ttggcggcaa gccgcgggtt gtcgtcgata tcgacggtcg
     1681 cgaccgtgac gcggtcggcg tagtcgttcg cgagcccttc gatgacgggc gcgatcgacc
     1741 ggcacggccc gcaccacgcg gccgagaagt cgacgacggc gggctgtgcc gagtcgagca
     1801 ccaagcgctc gaagtcgccc tcgcccacgc tcaacaacgc tgtcttcgga ttttccataa
     1861 cagtcactcc tttatatggt cggcaccggc gcggcttccc aaccgccgcc gagggccttg
     1921 tagatcagca cgaccgacga gttgagatcc gtctcggcgg ccgcgacctc gtcctcggct
     1981 tgcagcaagg tgcgctcggc gtcgagcagc cgcaggaaat ccacggcacc ctcgcggtat
     2041 tgaacgcgcg cgagctcggc cgcgttgcgc gacgccgcgg cctgctcgac gacggcttcg
     2101 agccgctgcc ggtgcgaccc gtacgtcacg aacgcgttct cggtctcttc gagcgcgcgc
     2161 agcaccgtga gctcgtaagt cgcgagcgct gcagccgtgc gcgcctcggc tacgacgacg
     2221 ttcgaatacg tgccgcgctc gaacccggcc cacgacaaga cgggcgaaag gctccacgcc
     2281 cggctcgtcg actcgccgag ctcgtcggca tcacccgcca cgaacccgat gaagcccgac
     2341 agcgtgaggc gcgggaacag atcggccttc gcgacaccga tgcgcgccgt cgcggctgcg
     2401 agctcacgtt cggctgcgcg aatgtcgggc cggcgttgca gcagcgccgc cggcgagtcg
     2461 acggcgatcg tcgtcaggtg cggcgcgatc tcccgcggcg cgagctccgc cgccagcgcg
     2521 ccggggcgct cgccgagcaa cacggcgagc cggttcgccg ctatcgtttc ggcggcctcg
     2581 agcggcggaa tcgtcgcctc ggtcgcggcg agccgcgcgg cggcgctcgc gacgtcgagc
     2641 tccgtgccgc gccccagctc gagccgcaca cgcgtcaggt cgagcgtctc ttgttgataa
     2701 cccaagttcg cgcgcgcgac gcccaagcgc ttctgcgcac cgcgcagctc gacgtagttg
     2761 cgcgcgacct cggccgctac gagcacctgc gcgtcgcgca agccggccac ggccgcatcg
     2821 gcgtcggcgc ccgcggcttc gacgccgcgc cgcacgcggc cgaacaagtc gagctcccac
     2881 agcgccgcga tacccaagtc atagctctcg acctccgtgc gactcgtcgt gaagcccggt
     2941 tgctgctgat ggccttccga gcggccgagc tcggccgacg cgcccggcca acgatcgcgc
     3001 cgagccgtgc cgaggtaagc gcgcgcttcc tgcacgcgtg ccgtcgcgac ggcgagatcg
     3061 aggtctgacg cgagcgagcg ctcgatcaag ctgtcgagcg agggatcgcg aaactgctgc
     3121 caccacgcgg cctcgaacgg ttgctgcgcg acgccctccg gtgtcgcgac gaacggcgtc
     3181 gcggcggtct cgggcgcgcg atagtcgggg ccgacggcgc acgccgcgag cagcgccgtg
     3241 accgcaatga cggatgtagt tctcatgaat ctctccctac gcctctacgg aatcaaagcg
     3301 gcggcggccg cgttcatgtc gttgatcgac cggcgctccg cgcgcgaacg cgcgccggtg
     3361 ccgagccgct ggacgagcca atagaacacc ggcgtcagca cgaggccgaa gatcgtgacc
     3421 ccgatcattc cggcgaagac ggcgacgccc atcgcgtggc gcatctcggc accggcgccg
     3481 tgcgcgagca cgagcggcac gacgccggcc gtgaacgcga tcgacgtcat caaaatcggc
     3541 cggaggcgca accgcgcggc gtcgatgatc gcgtcccagg tgctcgcgcc ggccagctca
     3601 cgctcacgcg cgaactcgac gatcaagatc gagttcttgg ccgcgaggcc gacgagcacg
     3661 atgagcccga tctgcgtgaa gatgttgttg tcgccgccca cgagccagac gcccgcgatg
     3721 gccgacaaca acgtcatcgg cacgatcagg atcacgacga gcggcagcgt caggctctcg
     3781 tagagcgccg cgagcacgag gaacacgagc acgacggaga tcggaaacac ccataagccg
     3841 gcgttgccgg ccagctgctt ctgatacgcg agctcggtcc actcgacgtg catgccgttc
     3901 ggcaacgacg ccttcgcgag ccccgcaatc agcgcctcgg cctcacccga cgagaagccc
     3961 ggcgccgggc cgccgttgat ctcggcactt ggatacccgt tgtagtgcat gacgcggtcc
     4021 gggccggccg cctgctcgag cgcgacgaac gagccgagcg gcaccatgcc gccctgcgca
     4081 ttgcgcacct tcagctggcc gatgtcctcg ggctcgagcc ggaacggcat gtcggcctgc
     4141 gcgttgacgc ggtacgtccg gccgaagcga ttgaagtcgt tgacgtacag cgagcccagg
     4201 tagacctgca tcgtgtcgaa gatctcgttc agcgcgacgc cctgtgccat cgccttgtcg
     4261 cgatcgacgt cggcgcgcac ctgcggcacg ttgacctgat agctcgagaa caagcccgtg
     4321 agccccgggg tttgccagcc ggcgccgacg atcttctgcg tctcctcgta cagggcctgg
     4381 taacccaagc cggcccgatc ctcgatctgc agcttgaaac cgccgatcgt gccgagacct
     4441 tgaatcggcg gcggctggaa gatcgcgatg tacgcgtctt gaatcgccgc gaatttctgg
     4501 ttcaacgcgg ccgcgacttc gttcgcagtg cgcgtcttct tgaagctcgg gtcctcccag
     4561 tcgtcaagcg caacgaaggc gatgccgacg ttcggcgcgt tgacgaagcc gttgacggag
     4621 agccccggga acgcaaccga atgcgcaacg ccgggctctg ccaacatgat gtccgtcatg
     4681 cggcggatga cgtcctcggt tcgatcgagc gttgcggctt cgggcaactg cgcgaacgcc
     4741 acgaggtact ctttgtcctg ctgcgggatg aaaccctccg gcacactctt gaagccgagc
     4801 caggcgacgc cgacgagcgc gacgtagatg actgccgccc cgcccgcacg cttcagcgtc
     4861 cggcccgtga gcccgacgta gtgcaccgac agccactcga agaagcggtt gaacggcccg
     4921 atggcccagc cgaacgcacg atcgatgccg cgttgaaaac ggtccggcgc ttcgccgtgc
     4981 ggcttcaaca gcaatgccgc gagcgccggc gacaacgtca gcgagttgat cgcggagatg
     5041 accgtgctga tggcgatggt cagcgcgaac tgctcgtaga attggccgct caagcccgtg
     5101 atgaacgccg tcggcacgaa cacggcaatc agcacggcgg agatcgcgat gatcgggctc
     5161 gtgacctcgc tcatggcctg cttcgcggct tcgatcggcc gcaggccctt ggcgatattg
     5221 cgctcgacgt tctcgacgac gacgatcgcg tcgtcgacga cgatgccgat cgcgagcacg
     5281 agaccgaaca gcgacagcgc gttcagcgaa aagccgagca gatgcatggc cgccagcgtg
     5341 ccgacgagcg acacgggcac ggccgcgagc gggatgaccg acgcccgcca ggtgcgtaag
     5401 aacagcacga cgacgagcac gacgagcagc agcgcctcga ccagcgtctg cacgacggcg
     5461 gcaatcgagt cgcgcacgaa cacggtcggg tcgtagacga tgcggtagtc gacgccctcg
     5521 gggaaatcct gcttgagctc cgccattttg gcgcggacct tgttcgagat gtcgatcgcg
     5581 ttgctgctgc cgggctgttg acgcaccggg attgcaaccg cgggctcgtt gtcgagcagc
     5641 gaccgcagcg catacgtgct cgcgccgagc tcgacgcgcg cgacgtcctt caagcgcgtg
     5701 atttggccgc gctcgcccgt tttgatgacg atgctctcga actcttcctc ggtcacgagg
     5761 cggccttgcg tgtcgacgag cagctggaac tgcgcggcgc tcgacgccgg cggactgccg
     5821 agctggccgg ccgcgacctg cacgttctgc gtccgaat
//
LOCUS       DATLEE010000531         3701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_615621, whole genome shotgun sequence.
ACCESSION   DATLEE010000531 DATLEE010000000
VERSION     DATLEE010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615621"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..744
                     /gene="nthB"
                     /locus_tag="VM692_11490"
     CDS             91..744
                     /gene="nthB"
                     /locus_tag="VM692_11490"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR03888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HUQ52836.1"
                     /translation="MNGVHDMGGMQGLGELHYARDDRAFHESWELRVHALTVAMAAHG
                     KWRVLRPKIEAIPAAEQLRMQYYERWLAALLEQTVDSGLATRAELERGRADSSAPRAS
                     PALTGAAAGEVPLRAPRTELNLDVAPRFAIGQTVRGRNLHPTTHTRMPRYTRGRVGVV
                     ERDRGVFALPDTDVYFLDPKPQHVYLVRFAARELWGEDAPARDSVYIDLWEDYLEPT"
     gene            731..1114
                     /locus_tag="VM692_11495"
     CDS             731..1114
                     /locus_tag="VM692_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HUQ52837.1"
                     /translation="MSRPDEARVRVELESLPRLPRDDRGPKFREPWEAQAFALAVKLH
                     AAGHFTWTEWCAELGAELKAAADRGAPDDGSRYFEHWLAALERLATAKKLVAGDELAQ
                     RKEAWTDAYRHTPHGRPVELAERRS"
     gene            complement(1111..1806)
                     /locus_tag="VM692_11500"
     CDS             complement(1111..1806)
                     /locus_tag="VM692_11500"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HUQ52838.1"
                     /translation="MKRLLVSCEHGANSVPKEFARQFVGAKDVLATHRGYDLGAADVA
                     RAFGRRLGVEPFIATTTRLVVDLNRSPGNRNVFSAYTRSLTPAQRGAALAAHYWPYRS
                     AVEDAVASAVARSGNVLHVSAHSFTPELRGEVRNCDVGFLYDPRRPGEVRFIEAWYAA
                     LEAAAPQLVLRRNYPYRGVSDALVTHLRRRYGMRGYLGMELELNQKHVGSRGWRRLVA
                     VLGATLVTACDAV"
     gene            complement(1803..3107)
                     /locus_tag="VM692_11505"
     CDS             complement(1803..3107)
                     /locus_tag="VM692_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HUQ52839.1"
                     /translation="MPASNSNPPPDDGDAPARPLRLFEAYGLELEYMLVDGESLDIAP
                     AADQLLEAAAGELTDEFFNGEIAWNNELALHVIEFKCNGPRPSLAGLGAAFAANVKLA
                     NDKLGREGLRLMPTAMHPWMDPNDVELWPHGTRVIYDTFDRIFSCKGHGWANLQSQQI
                     NLPFAGDEEFAQLHAAIRFLLPILPGLAASSPVVDGERNGILDNRLVVYRSNCARIPS
                     ITGDVVPEPVGSIGEYHERVLEPIYRDLAPHYPDGVLRNEWVNARGAIARFDRMAIEI
                     RVLDVQETPVVDVAYAALIVEILKLLAAEQWLDRDGMNSWRTAELGKLFTLASRQGEG
                     AGIGDKRFLAAFGLRSSGVELKGLWEHLIETVSARGSLDAQTGRLLEHYLRHGTLATR
                     IGSSLGLLPTRAKLARVYEELCEALADSRPFTPPAPRREPLT"
     gene            complement(3076..>3701)
                     /locus_tag="VM692_11510"
     CDS             complement(3076..>3701)
                     /locus_tag="VM692_11510"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HUQ52840.1"
                     /translation="VYLVELAKRLELAIPPTMVVGSSTEVDDIIAELGLPCVLKQPDS
                     AFSQGVFRADTRAALAEGLERLLSRSDLVVVQSFMPTEFDWRVGVFDRQPLFVCRYFM
                     AEGHWQIYHHGEKRTTEGESETIAVQHAPREVIQLALKTANAIGDGFYGVDIKTVNGR
                     HVLMEVNDNPNVDRGVEDAILGDALYDRIMKGLRARVDAGKQFKPAA"
BASE COUNT          584 a         1263 c         1299 g          555 t
ORIGIN      
        1 gcacgagctc acggaagccg agctcgcgga gctcgtcacg cgcgacgcga tggtcggcgt
       61 cgcgcgcgct ctcgcgcctg aagtgcgcag atgaacggcg tccatgacat gggcggcatg
      121 cagggcctgg gcgagctgca ctacgcgcgc gacgaccgcg cgttccacga gtcatgggag
      181 ctgcgagtgc acgcgctgac ggtcgccatg gcggcccatg gcaaatggcg cgtgctgcgc
      241 cccaagatcg aagcgatccc cgcggcagag cagctgcgaa tgcaatacta cgagcgttgg
      301 ctcgcggcgc tcctggagca gacggtcgac agcggcctcg cgacgcgcgc cgagctggag
      361 cgcggccgtg ccgactcgag cgcgccgcgt gcctccccgg ccctcacggg cgcagcggcc
      421 ggcgaagttc cgctgcgcgc gccgcgtacg gagctcaacc tcgacgtcgc gccgcggttc
      481 gccatcggcc aaaccgttcg cggccgcaat ctgcatccga cgacgcacac gcggatgccg
      541 cgctacacgc gcggccgagt cggcgtcgtc gagcgcgacc gcggcgtatt cgcattgccg
      601 gacaccgacg tgtacttcct cgacccgaag ccgcagcacg tctatctggt ccgcttcgcg
      661 gcgcgcgagc tctggggaga agacgcgccg gcacgcgact ccgtctacat cgatctctgg
      721 gaagactacc ttgagccgac ctgacgaagc ccgcgtccgc gtcgagctcg agtcgctgcc
      781 gcgcctgccg cgcgacgatc gcggccccaa gttccgcgag ccgtgggagg cgcaggcctt
      841 cgcgctggcc gtgaagctgc acgcggcagg ccacttcacg tggacagagt ggtgcgccga
      901 gctcggagcc gagctcaaag cggcggcgga ccgcggcgcc cccgacgacg gttcgcgcta
      961 cttcgagcat tggctcgccg cgctcgagcg cttggcgacg gcgaagaagc tcgtcgcggg
     1021 cgacgagctg gcgcagcgca aggaagcttg gaccgacgcc tatcgacaca cgccgcacgg
     1081 ccgacccgtc gagctcgccg agcggcgcag ctagacggcg tcgcaggccg tcacgagcgt
     1141 ggcgccgagc acggccacga gccggcgcca gccgcgactg ccgacgtgct tctgattgag
     1201 ctcgagctcc atgccgagat aaccgcgcat gccgtagcgc cgccgcaagt gggtcacgag
     1261 cgcgtccgag accccgcgat acgggtagtt gcgccgcagc acgagctggg gcgccgcggc
     1321 ctcgagagcc gcataccacg cctcgatgaa gcggacctcg ccgggccggc gcgggtcgta
     1381 gaggaaaccg acgtcgcagt tgcggacctc gccgcgcagc tccggcgtga agctgtgcgc
     1441 ggaaacgtgc agcacattcc cgctgcgtgc aaccgcgctg gcgacggcgt cctcgacggc
     1501 gctgcgataa ggccagtagt gggcagcgag cgcggcgccg cgctgcgccg gcgtcagcga
     1561 gcgcgtgtac gccgagaaca cgttgcgatt gcccggcgag cggttcaagt cgacgacgag
     1621 ccgtgtcgtc gtcgcgatga acggctcgac gccgaggcgt cggccgaacg cgcgagcgac
     1681 gtcggccgca cccaagtcgt agccgcggtg cgtcgcgagc acgtccttgg cgccgacgaa
     1741 ctgccgcgcg aattccttcg ggacgctgtt ggcgccgtgc tcgcagctga cgaggaggcg
     1801 tttcatgtca gcggctcgcg ccgcggcgcg ggcggagtaa acggccggct gtcggccagc
     1861 gcctcgcaga gctcttcgta gacgcgtgcg agcttcgcgc gcgtcggcag gagccctaga
     1921 ctcgagccga tgcgcgtcgc gagcgtgccg tggcgcagat agtgctcgag caggcggccc
     1981 gtctgggcgt caagcgagcc acgggccgag acggtctcga tcaagtgctc ccagagacct
     2041 ttgagctcga cgccgctgct gcgcagaccg aacgccgcca ggaaccgttt gtcgccgatg
     2101 ccggcgccct cgccttgccg cgacgccagc gtgaacagct tgccgagctc ggcggtgcgc
     2161 cagctattca tgccgtcgcg gtcgagccat tgctcggccg cgagcagctt cagaatctct
     2221 acgatcagcg ccgcgtatgc gacgtcgacg acaggcgttt cctgcacgtc gagcacgcgg
     2281 atctcgatcg ccatgcggtc gaagcgcgcg atcgcaccgc gcgcgttgac ccactcgttg
     2341 cgcagcacgc cgtcgggata gtgcggcgcg agatcacggt aaatgggctc gagcacgcgc
     2401 tcgtggtatt cgccgatcga gcccaccggc tcgggcacga cgtcgccggt gatcgacgga
     2461 atccgcgcgc aattgctgcg gtagacgacg aggcgattgt cgaggatgcc gttgcgctcg
     2521 ccgtcgacga cgggcgagct cgcggcgagg cccggcagga tcggcaacag gaagcgaatc
     2581 gcggcgtgca gctgcgcgaa ctcctcgtcg ccggcaaacg gcaggttgat ctgctggctt
     2641 tgtagattgg cccagccgtg gcctttgcac gagaagatgc ggtcgaacgt gtcgtagatg
     2701 acacgcgtgc cgtgcggcca cagctcgacg tcgttcggat ccatccacgg atgcatcgcc
     2761 gtcggcatca ggcgcaagcc ctcgcggccg agcttgtcgt tcgcgagctt gacgttggcg
     2821 gcgaatgccg cgccgaggcc ggccagcgac ggccgcggcc cgttgcactt gaattcgatg
     2881 acgtgcagcg cgagctcgtt gttccaggcg atttcgccgt taaaaaattc gtcggtcagc
     2941 tcgccggcgg cggcctcgag cagctgatcc gcggccggcg cgatgtcgag gctctcgccg
     3001 tcgacgagca tgtactcgag ctcgagcccg tacgcctcga acaggcgcag cggccgcgcg
     3061 ggcgcgtcgc cgtcgtcagg cggcgggttt gaattgcttg ccggcatcga cgcgtgcgcg
     3121 cagccctttc atgatgcgat cgtagagcgc gtcgccgagg atcgcgtcct cgacgccgcg
     3181 gtcgacgttc gggttgtcgt tgacctccat gaggacatgg cggccgttga cggtcttgat
     3241 gtcgacgccg tagaagccgt cgccgatcgc gttggccgtc ttcaacgcga gctggatgac
     3301 ctcgcgcggc gcgtgctgca cggcgatcgt ctcggactcg ccttcggtcg tgcgcttctc
     3361 gccgtggtga tagatctgcc agtgcccctc ggccatgaaa tagcggcaga cgaacagcgg
     3421 ttggcggtcg aacacgccga cgcgccagtc gaactccgtc ggcatgaacg actgcacgac
     3481 gaccaagtcg ctgcgcgaca gcagtcgctc gagcccttcg gcaagcgcgg cgcgcgtgtc
     3541 ggcgcggaac actccttgcg agaacgcgct gtcgggctgc ttcagcacgc acggcaggcc
     3601 aagctccgcg atgatgtcgt cgacctccgt gctgctgccg acgaccatcg tcggcggaat
     3661 cgcgagctcg agccgcttcg cgagctcgac gagatagacc t
//
LOCUS       DATLEE010000532         2792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4316571, whole genome shotgun sequence.
ACCESSION   DATLEE010000532 DATLEE010000000
VERSION     DATLEE010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4316571"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VM692_11515"
     CDS             <1..712
                     /locus_tag="VM692_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ52841.1"
                     /translation="DDRGPLIMPPAVYEALIVALDRAGIQVHVHSIGDRGARVTLDAF
                     AAARERNGADRPMHQMAHLQLVHPADIARFAALRVAANIQGLWAYHDEETIELVDPAI
                     GPERGGRLYPYGSLERAGATLVGGSDWSVTSMNPLPAIEIAVTRRGARQPAGPAWLPN
                     EVVSLDAMLLAYTINGARLQLHDAEVGSITVGKAADLVVLDKDLRSIPVVDINTARVL
                     YTFVDGVQVHPPRDGRPQ"
     gene            885..2534
                     /locus_tag="VM692_11520"
     CDS             885..2534
                     /locus_tag="VM692_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HUQ52842.1"
                     /translation="MAARNGIGLDRRSFLGATVAAGASLAFSGLTLSAAAQDRRLTTV
                     GGTAKTQYGSVRGLLKDGVQQFWCVPYGASTAGANRFMPPQKPASWSGVKDHFEITWA
                     APMDPTGEEPSPVVTALNRKTPQSEDCLTVNVFTPGVDNKARPVMVWMHGGGFSAGSG
                     NYLLYDGTNLAKKEDVVVVSVNHRLNIFGYLHLAGLGGGEKWARSTNVGVQDLVAALQ
                     WVKDNIENFGGDPSRVTIFGQSGGGGKTTTVMAMPSARGLFHRSIAQSGSAIRGQSAD
                     DAADGAERFLAKLGIQKNQLERVQQVDWHDIQKAFYSEPRIARLGNGPVIDGTIIPRH
                     QWDPTAPSYSANVPFMAGSVENEDGWVGPPPYELPDDEMLELFTTRLASNDAAEGQKL
                     LALYKRRHPETRNRMLWLMADSDDTRRWNAQLLCRLKYEQGTAPSYLYFFNWQSPVHN
                     NRMGAYHTLDIPFVFYNMDIGASMTGSAQSRYQLGHVMSAAWAAFARTGNPNHADMPN
                     WPTFEPNTLPTMVFGEKVAVVNDPNKEERLALAELRKKRPS"
     gene            2554..>2792
                     /locus_tag="VM692_11525"
     CDS             2554..>2792
                     /locus_tag="VM692_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52843.1"
                     /translation="MRSSRTLLALAVAAAFTAALSGAQAQNRQTGGTVEDQFVLPVPE
                     VEKPPREFKTAEEHYNYLLERAHGGTKHTMQTIPV"
BASE COUNT          500 a          924 c          928 g          440 t
ORIGIN      
        1 cgacgaccgg ggtccgttga tcatgccgcc cgccgtttac gaagcgttga tcgtggcgct
       61 cgatcgtgcg ggaatccagg tccacgtgca ctcgatcggc gatcgcggcg cgcgcgtcac
      121 gctggatgcg ttcgccgcgg cacgcgagcg caacggcgct gacaggccga tgcatcagat
      181 ggcgcatctg caactcgtcc accctgccga catcgcacgc ttcgccgcgc tgcgcgtcgc
      241 tgcgaacatt cagggcctct gggcttatca cgacgaggag acgatcgagc tcgtcgaccc
      301 ggccatcggt cccgagcgcg gcgggcgcct atatccttac gggagtctcg agcgcgccgg
      361 cgccacgctc gtcggcggca gcgactggtc tgtgacgtcg atgaacccgc tgccggcgat
      421 cgagattgcc gtcacgcgcc gcggcgcgcg ccagccggcc gggccggctt ggttaccgaa
      481 cgaagtcgta agcctcgacg cgatgctgct ggcctatacg atcaatggcg cgcgcctgca
      541 gctgcacgac gcagaagtcg gttcgatcac cgtggggaag gcggccgacc tcgtcgtgct
      601 cgacaaggac ttgcgctcga ttcccgtcgt cgacatcaac accgcacggg tgctctacac
      661 gttcgtcgac ggcgtccaag tgcatccgcc acgcgacggt cggccgcagt gattcgatgc
      721 ttcgagcgta cgacgcgctg ggccgcataa cgttgaacag gccggcggcg ccacgtcgaa
      781 ctcccaagtt ttgccgagcc gaaccgcgcg gtagcgcgct tcgccgggcg gttgtcgtat
      841 agtcggccga agccaactgg acaatcagaa attaggggga tgcgatggct gcacggaacg
      901 gcatcggact ggatcgccgg agcttcttgg gcgcgactgt cgcggcgggc gcgagcctcg
      961 ccttcagcgg tttgacgctt agcgccgcgg cgcaggaccg gcggctcacg accgtcggcg
     1021 gcacggcgaa gactcagtac ggcagcgtgc gcggcctcct caaggacggc gtgcagcaat
     1081 tctggtgcgt gccgtacggc gcgtcgacgg ccggtgcgaa ccgcttcatg ccgccgcaga
     1141 agccggcgtc gtggtcgggc gtcaaagacc acttcgagat tacctgggcc gcgccgatgg
     1201 atccgaccgg cgaggagccg tcgcccgtcg tcacggcgct gaaccgcaag acgccgcaga
     1261 gcgaggattg cctcaccgtc aacgtcttca cacccggcgt cgacaacaaa gcgcgccccg
     1321 tcatggtctg gatgcacggc ggcggcttct cggccggctc cggcaactac ttgctctacg
     1381 acggcacgaa tctcgcgaag aaggaagacg tcgtcgtcgt ttcggtcaat caccgcttga
     1441 acatcttcgg ctacctgcac ctcgcgggcc tgggcggcgg cgagaaatgg gcgcggtcga
     1501 cgaacgtcgg cgtgcaagat ctcgtcgctg cgctgcagtg ggtcaaggac aacatcgaaa
     1561 acttcggcgg cgaccccagc cgcgtgacga tcttcggtca gtccggcggc ggcggtaaga
     1621 cgacaaccgt catggcgatg ccgtcggcgc gcggtctgtt ccatcgctcg atcgcgcaga
     1681 gcggctcggc gattcgcggc cagtcggccg acgatgcggc cgacggcgcc gagcgtttcc
     1741 tcgcgaagct cggcatccag aagaatcagc tcgagcgcgt gcagcaggtc gattggcacg
     1801 acatccagaa ggcgttctac agcgagccgc gcatcgcgcg tctcggcaac ggtcccgtca
     1861 tcgacggcac gatcattccg cgccaccagt gggacccgac ggcgccgtcg tactcggcga
     1921 acgtgccgtt catggcgggc tccgtcgaga acgaggacgg ctgggtcggg ccgccgccgt
     1981 acgagctgcc cgacgacgag atgcttgagc tcttcacgac gcggctagca agcaatgacg
     2041 cggccgaagg ccagaagctg ctagcgctct ataagcgcag gcacccagag acgcgcaacc
     2101 gcatgctgtg gctgatggcc gattccgacg acacgcgccg ctggaacgcg cagctgctgt
     2161 gccgcttgaa atacgagcaa ggcacggcgc caagctatct ctacttcttc aattggcaat
     2221 cgcccgtgca caacaaccgt atgggcgcgt accacacgct ggacattccg ttcgtgttct
     2281 acaacatgga catcggcgcc tcgatgaccg gctcggcgca gtcgcgctat cagctcggcc
     2341 acgtcatgag cgcggcgtgg gccgcgtttg cgcgcacggg caatccgaac catgccgaca
     2401 tgccgaattg gccgacgttc gagccgaaca ccctgccgac aatggtgttc ggcgagaagg
     2461 tcgccgtcgt caacgatccg aacaaggaag agcggctcgc gctcgcggag cttcgcaaga
     2521 agcggccgtc gtaacaatcc gagggggtgc gtcatgcgca gcagcagaac tctcctggct
     2581 ttggccgtcg ccgccgcgtt cacggcggcg ctgagcggcg cgcaagcgca gaaccggcag
     2641 acgggcggta ccgtcgagga tcagttcgtc ttgccggtgc ccgaggtcga gaagccgccg
     2701 cgcgagttca agaccgccga agagcactac aactatctgc tcgagcgcgc gcacggcggc
     2761 acgaagcaca cgatgcagac gatccccgtg tg
//
LOCUS       DATLEE010000533         6814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3302198, whole genome shotgun sequence.
ACCESSION   DATLEE010000533 DATLEE010000000
VERSION     DATLEE010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3302198"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="VM692_11530"
     CDS             <1..1532
                     /locus_tag="VM692_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52844.1"
                     /translation="VPAGAINASTGAGTGANGTGRVNVTAIAVPANGTVSVVFDARIG
                     AGTAPMTPLNNTATIANPGGPGASPAAPTLLVSPSALPSSGAKPLYLRSAPAVALSRN
                     PPGADAEVTVVFGAPIAWTLAPTLTLPTTLSAGNIAVPLWLRRNNNTGTNSRTVQVTL
                     ANTVTGTIGSATQTLTTLPSAGSPALVNFVLPNPAAATFPAGSAFRVTITQTAPNVAG
                     RTTLVYPVGATAGSYSRAVLNSATVVNVDSVQTFNAAYAGGVAQGSFNVGNTVYVRAV
                     ISDPFGSFDISAARISITDAASVVRLASAALPQVADSGAATRTYEYAYVVPAGAPPGG
                     WTVQVTGVEGTEATVTDTRVGGFVVLPNLPALRVTKTVDVLSDPVNGAVNPRQIPGSV
                     QRYRVAVANSGVGAVDASTLVIADIIPANAELVVSGGNAVQFFDGAVSSNLTFNYATH
                     VTFSNQPGGAPPYTYVPVANGNGVDPAVTALRVAPSGAMAGASGANQPSFSVEFRVRV
                     R"
     gene            1578..6191
                     /locus_tag="VM692_11535"
     CDS             1578..6191
                     /locus_tag="VM692_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUQ52845.1"
                     /translation="MRTFHPAVDKWFHAVFAEATAPQKRGWPPIARGESTLILAPTGS
                     GKTLTAFLWSLNRLMFEPVPPPAERCRVLYISPLKALAVDVERNLRAPLAGISNVAAS
                     QGVAFHAPAIAVRTGDTPAAERARFQKTPADILITTPESLFLLLTSNAHESLRSGETV
                     IIDEIHAVVATKRGAHLALSLERLERIARSQPLQRIGLSATQRPLDEVARYLGGATAS
                     TDSAKAAPRTASTCGSEERAPDDFAASVHAEFEDDHAVQYRPVTIVEASAPTPLAISV
                     QTPVEDMAQIGKPVEQPSGPASRGPVAQSIWSSIHPKLVELIRSHRATLIFVNSRRLA
                     ERLAGALNELAGEALVRAHHGSLARSQRNEIEDLLKSGHLRALVATSSLELGIDMGAI
                     DLVIQLEAPPSVASGLQRIGRAGHSIGQVSEGVIFPKYRGDLLACAAVTAAMRSGKVE
                     SSRYPRNPLDVLAQQLVAMASMELWSVDELYAAVRSAAPYAELSRPMFEGVLDMLSGR
                     YPSDEFAELRPRVTWDRVNNTVVARQGAKRVAIANAGTIPDRGLYGVFLSGAEKDKAR
                     VGELDEEMVFEARAGETFLLGASTWRIDQITHDRVLVSPAPGEPGKMPFWKGEGPGRP
                     LELGMAIGKLVRDLRKQTPAEAVATLAQGHGLDALASANLLRYLADQSDATGEIPDDR
                     TAVIELSRDELGDWRVAVLTPFGSRIHAPWAMAVTARVRDETGLDVEVMWSDDGFVVR
                     YPDGERPPDTSLLLIDPDEVERLVVRQLGSTAMFAARFREAAGRALLLPRRRPGARAP
                     LWQLRKRAADLLAVAARYGSFPIILETYRECLRDIFDLPALTDLLRRIRTRSMRVVTV
                     ESRTPSPFAASLLFGYVANYLYDGDAPLAERRAQALSVDQSQLRDLIGDTELRDLLDQ
                     EVLENLENELQHLPPRFHARSIDAVHDLLLRLGDLSPNEIAARSEAGVAARAIEELVR
                     ARRAVRVRIAAEERLIAAEDSARYRDAIGVPLPTGLPLSLLTPVADPVTDLVRRFGRT
                     HGPFTVKDVGARLGLGSAVVDAALARLRANGRLLDGEFRPGGRGREWCDAEVLRIVRQ
                     RSLARLRQDVEPVEAQALGRFLVRWHSLARRRHGLDGLLDAIEQLQGVPLAASVLESE
                     VLTARVQEYAPAMLDTLLGAGEVTWIGIEALGERDGRVALYLTDHVAKLLPPGAPGPK
                     PDDELSTRETAIVAYLGEHGASFFQQIHDAIGGGFPPQTVDAIWNLVWRGLLTNDTLH
                     ALRAYAAAPERSRRQARGGAFRSRRLVPRSAEGRWTLVVAPRESATTWATSMANQLLA
                     RHGVVTRDVAAIERLPGGFAALYPVLRRLEETGRIRRGYFVAGLGAAQFAQPGAVDLL
                     RDAREDGDEVVATVISATDPANPYGVTVPWPAPASEDTRGATRTAGARVVLVNGMMAA
                     WISRGDRQLIVCLPDDEPERSQIGRALARELKAIAERASGRRRGWLIEDINGVQSVRH
                     PASRFLIEAGFAATAKGLQLRVPRGRSIEESAAAPDPRLRAGG"
     gene            6237..>6814
                     /locus_tag="VM692_11540"
     CDS             6237..>6814
                     /locus_tag="VM692_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HUQ52846.1"
                     /translation="MHRVLAGNVVTRFETAYAHLDRVDVDTPLVGRTIERVESAGKHH
                     LIVFSGGLVLRTHMRMNGSWHLYRHGEKWWRSRQAMRVRIDTDDWVAVAFDVPIAEFV
                     TPKQLATTDPVAKLGPDLLGREFDVGEAARRLIESRDRPIAMSLLDQRVVAGLGNVYK
                     SEVLFLSGLNPFTPTCDVPRETLVRMLELARR"
BASE COUNT         1046 a         2315 c         2353 g         1100 t
ORIGIN      
        1 gcgtgccggc cggagcgatc aatgcttcga cgggcgcggg cacgggcgcg aacggcacgg
       61 gccgcgtcaa cgtgacggct atcgccgtgc cggcgaacgg taccgtgtcg gtcgtgttcg
      121 acgcgcgcat cggcgccggc actgcgccga tgacgccgct caacaacacg gcgacgatcg
      181 cgaatcccgg cgggcccggt gcttcgccgg cggcgccgac gctgctcgtg tcgccgtcgg
      241 cgctgccgtc gtcgggcgcg aagccgctgt atcttcgcag cgcaccggcc gtcgcgctgt
      301 cgcgtaatcc gccgggcgcg gatgccgaag tgacagtggt gttcggcgcg ccgatcgcat
      361 ggacgttggc gccgacgctg acgctcccga cgacgctgtc ggccggcaac atcgccgtgc
      421 cgctctggct gcgccgcaac aacaataccg gcaccaacag ccgcaccgtc caagtgacgc
      481 tggccaacac ggtcacgggc acgatcggca gcgcgacgca gacgctgacg acgttgccgt
      541 ccgccggctc gccggcgctc gtgaacttcg tactaccgaa tccagccgcg gcgacgttcc
      601 ccgcggggtc ggcgttccgc gtcacgatca cccagaccgc gccgaacgtc gcgggccgca
      661 cgacgctcgt ctatcccgtc ggcgcgacgg cgggcagcta cagccgcgcc gtgctgaaca
      721 gcgcgacggt cgtcaacgtc gacagcgtgc agacgttcaa tgccgcgtac gccggcggtg
      781 tcgcgcaggg cagcttcaac gtcggcaaca ccgtctacgt gcgcgccgtc atcagcgatc
      841 cgttcggcag cttcgacatt tcggcggcgc gcatttcgat caccgacgcg gcgtccgtcg
      901 tccgcctcgc cagcgcggcg ctgccgcagg tcgccgactc gggcgctgcg acgcgaactt
      961 acgaatacgc ctacgtcgtg ccggccggcg cgccgccggg cggctggacc gtgcaagtga
     1021 cgggcgtcga aggcacggaa gccacggtca cggatacgcg cgtcggcggc ttcgtcgtgt
     1081 tgccgaacct acccgcgttg cgcgtcacga agaccgtcga cgtgctctcg gatccggtca
     1141 acggcgccgt caatccgcgg caaatcccgg gcagcgtgca gcgttaccgc gtcgccgtcg
     1201 cgaacagcgg cgtcggtgcc gtcgatgcca gcacgctcgt gatcgccgac atcattcccg
     1261 cgaacgccga gctcgtcgtg tcgggaggca atgcagtcca gttcttcgac ggcgccgtgt
     1321 cgagcaacct gacgttcaac tatgcaacgc acgtgacgtt ctcgaaccag cccggcggtg
     1381 cgccgcctta cacctacgtg cccgtcgcga acggcaacgg cgtcgatccc gcggtcacgg
     1441 cgctgcgcgt cgcgccgagc ggcgcgatgg ccggcgcgtc gggcgccaat cagccgagct
     1501 tcagcgtcga gttccgcgtc cgcgtgcgtt gaaggtccgc gctcggcctg tggcatacct
     1561 tgtgcacgca aagcggcgtg cgcacgttcc atccggccgt cgacaagtgg ttccatgccg
     1621 tattcgcgga agcgacagcg ccgcaaaagc gcggctggcc gcccattgcg cgcggcgagt
     1681 cgacgttgat tcttgcgccg acgggtagcg gcaaaacgct gacggccttc ctgtggtcgc
     1741 tgaatcggct gatgttcgag cccgtgccgc cgcccgcgga acggtgtcgc gtcctctaca
     1801 tctcgccgct gaaggcgctg gccgtcgacg tcgagcgcaa cctgcgtgcg ccgctcgccg
     1861 gcatttcgaa cgtcgccgcg agccaaggcg ttgcgtttca cgcgccggcg atcgccgtgc
     1921 ggacgggcga cacgccggca gccgagcgcg cgcgattcca aaagacgccc gccgacatct
     1981 tgatcaccac gccggagtcg ctattcctgt tgctgacgtc gaacgcgcac gagagcttac
     2041 ggtccggcga gaccgtgatc atcgacgaga tccacgccgt cgttgccacc aagcgcggcg
     2101 cgcaccttgc gctgtccctc gagcgtctcg agcgtattgc gcgctcgcag ccgctgcagc
     2161 gcataggact gtccgcgacg cagcggcccc tcgacgaggt cgcgcgctac ctcggaggcg
     2221 ctacggcgtc gacggactca gccaaagcgg ctccgcgcac agcgtcgaca tgcgggagcg
     2281 aagagcgcgc gcccgacgat ttcgccgcaa gcgttcacgc agagttcgaa gacgatcatg
     2341 ccgtgcagta tcgacccgtc acgatcgtcg aggcgagcgc gccgacgccg cttgcgatca
     2401 gcgtgcaaac gccggtcgag gacatggcgc agatcggcaa gcccgtggag caaccgagcg
     2461 gtccggcgtc acgcggaccc gtcgcgcaat cgatctggag ctcgatccat ccgaagctcg
     2521 tcgagctgat ccgttcgcat cgcgcgacgc tgatcttcgt caacagccgc aggttggcgg
     2581 agcggctcgc aggcgctttg aacgagcttg ccggcgaagc gctggtacga gcgcaccacg
     2641 ggtcgcttgc gcgctcgcag cgcaacgaga tcgaggatct cttgaagagc ggacacctgc
     2701 gagcgctcgt ggccacttcg tcgctggagc tcgggatcga catgggcgcg atcgatctag
     2761 tgatccaact cgaggcgccg ccgtcggtcg cgagcggcct gcagcgcatc ggccgggccg
     2821 gtcacagcat cgggcaagtc agcgaaggcg tgatctttcc caagtatcgc ggcgacctgc
     2881 tggcgtgtgc cgcggtgacc gcggcgatgc gcagcggcaa ggtcgagtca tcgcgctatc
     2941 cacgcaatcc gctcgacgtg ctcgcgcagc agctggtggc gatggcgtcg atggagctct
     3001 ggagcgtcga cgagctctat gccgcggttc ggtcggcagc gccgtacgcc gagttgagcc
     3061 ggccgatgtt cgaaggcgtg ctcgacatgc tctcgggacg ttatccgtcg gatgaattcg
     3121 cggagctcag gccgcgcgtc acgtgggacc gcgtcaacaa cacggtcgta gctcgacagg
     3181 gtgcgaagcg cgtggcgatc gccaacgccg gtacgattcc ggatcgcggc ctgtacggcg
     3241 tgttcctgtc gggcgccgaa aaggacaagg ccagagtcgg cgagctcgac gaagagatgg
     3301 tattcgaggc cagggccggc gaaaccttcc tgctcggcgc ctcgacctgg cgcatcgatc
     3361 agatcacgca cgatcgagtg ctcgtttcgc cggctcccgg cgagcccggc aagatgccgt
     3421 tctggaaagg cgaagggccc ggccggccgc tcgagctcgg catggcgatc ggcaagctcg
     3481 tgcgcgactt gcgcaagcag acgccggccg aggccgtcgc gacgctcgcg caaggccacg
     3541 gtctggacgc gctcgcaagc gcgaatctgc tccgctacct cgccgaccag agcgacgcta
     3601 cgggcgagat ccccgacgac cggaccgcgg tgatcgagct atcgcgcgac gagctcggcg
     3661 actggcgcgt cgcggtgctg acgccgttcg gtagccgcat ccacgcgccg tgggcgatgg
     3721 ccgtgacggc gcgcgtacgc gacgaaacag gcctcgacgt cgaggtcatg tggtcggacg
     3781 acggcttcgt cgtgcggtat cccgatggcg agcggccacc cgatacgtcg ttgctgctga
     3841 tcgaccccga tgaggtcgag cgcttggtcg tgcgccagct cggctcgacg gcaatgttcg
     3901 cggcccgatt tcgcgaggcc gccggccgcg cgttgctgct gccgcggcgc cggcccggcg
     3961 cgcgagcgcc gctgtggcaa ttgcgcaagc gcgcggcgga cttgctcgcg gttgcggctc
     4021 gctacggatc gtttccgatc atcctcgaaa cctaccgcga atgtctgcgc gacatcttcg
     4081 atctgccggc gctgaccgac ttgctgcgac gcatacgcac ccgcagcatg cgcgtcgtca
     4141 ccgtcgagtc ccgtacaccg tcgccgttcg cggcgtcgct gttgttcggc tacgtggcca
     4201 actacctcta cgatggcgac gcaccgctcg cggaacgccg cgcgcaggct ttgtccgtcg
     4261 accagtcgca gttgcgcgac ctgatcggcg acaccgagct gcgcgatctg ctcgatcagg
     4321 aggtcctcga gaacctcgag aacgagctgc agcacctgcc gccgcgtttc catgcgcgct
     4381 cgatcgacgc cgtgcacgac ctgctgctgc ggctcggcga tctttcgcct aacgagatcg
     4441 cagcgcgcag cgaggccggt gttgcggccc gcgccatcga agagctcgta cgagcgcggc
     4501 gtgccgtccg cgtgcgcatt gcagccgagg agcgcttgat cgccgccgag gactctgcgc
     4561 gctatcgcga cgcgatcggc gtgccgctgc cgaccggttt gccgctgtca ctgctcacgc
     4621 ccgtggcgga cccggtcacc gatctcgtgc gtcgcttcgg ccgcactcac ggcccgttca
     4681 ccgtcaagga cgtcggcgcg cggctcgggc tcggcagcgc cgtcgtcgac gcggcgctcg
     4741 cgcgcttgcg cgccaacggc cggttgctcg acggtgaatt tcgccccggc gggcgaggcc
     4801 gagagtggtg cgacgccgaa gtgctgcgaa tcgtgcggca gcgttcgctc gctcgtttgc
     4861 gccaggacgt cgaaccggtc gaggcgcagg cgctcggccg ttttctcgtg cgctggcaca
     4921 gcttggcgcg ccgtcgccac gggctcgacg gactgctcga cgcgatcgag caactgcaag
     4981 gcgtcccgct cgcggcgtcg gtgctcgaaa gcgaagtgct gacggcgcgc gtgcaggagt
     5041 acgcgccggc catgctcgac acgttgctcg gcgccggcga agtgacttgg atcggtatcg
     5101 aggcgctcgg cgagcgcgat ggccgagtcg cgctgtattt gacggatcac gtcgcgaagc
     5161 tgttaccgcc gggtgcgccc gggccaaagc cggacgacga gttgtccacg cgcgaaaccg
     5221 ccatcgtggc ctacctgggc gagcatggtg cttcgttctt ccagcagatt cacgacgcga
     5281 tcggcggcgg atttccgccg cagacggtcg acgccatctg gaatctcgtt tggcgcggtt
     5341 tgctaaccaa cgacaccctg catgccttgc gcgcgtatgc agcggcgccc gagcggtcgc
     5401 gccgccaagc gcgcggcgga gcttttcgct cgcgccggct cgtgccgcgc tcggccgaag
     5461 gccgttggac gctcgtcgtc gcgccgcggg agtccgcgac gacctgggca acctcgatgg
     5521 caaatcaact gctcgctcgg catggcgtgg tgacgcgcga cgtggcggcg atcgagcgat
     5581 tgccgggcgg ctttgcggcg ctgtatcccg tgctacggcg tctcgaagag acggggcgca
     5641 tccggcgcgg ctatttcgtc gctggcttag gtgcggcgca gttcgcacag ccgggtgccg
     5701 tcgatctcct gcgcgatgcg cgcgaggacg gcgacgaagt cgtcgcaacc gtcatttccg
     5761 ccaccgatcc tgcgaacccg tacggtgtga cggtcccatg gccggcaccg gcatccgagg
     5821 acacgcgcgg tgcaacgcga acggccggtg cgcgcgtcgt gctcgtcaac ggcatgatgg
     5881 ccgcgtggat cagccgtggc gatcggcagc tgatcgtgtg tctgcccgac gacgagccgg
     5941 agcggtcaca gatcggccgt gcgctcgcgc gggagctcaa agcgattgca gaacgggcat
     6001 cggggcggcg gcgcggctgg ttgatcgaag acatcaacgg cgtacagtcg gtccgccatc
     6061 ccgcgagccg ctttctgatc gaggcgggtt ttgcggcgac ggcgaagggc ttgcagctgc
     6121 gcgtgccgcg cggccgctct atcgaggaaa gcgccgcggc gcccgacccg aggctccggg
     6181 ccggcggata acgcgccatg ccggaaggcg acacgatatt tcgcgctgcg cggtcgatgc
     6241 accgagtctt ggccggtaac gtagtgacgc gattcgagac ggcgtatgcg catctcgatc
     6301 gagtcgacgt ggacacgccg ctcgtgggcc gcacgatcga gcgcgtcgag tcggccggca
     6361 agcatcactt gatcgttttc agcggcggct tggtgttgcg tacacacatg cgcatgaacg
     6421 gctcgtggca tttgtatcgg cacggcgaaa agtggtggcg aagccggcaa gccatgcgcg
     6481 tccgtatcga taccgacgac tgggtggcag ttgccttcga cgtgcccatc gcggaattcg
     6541 tgacccctaa gcagctcgcg acgacggatc cggtcgcgaa gctcgggccg gatctgcttg
     6601 gccgcgagtt cgacgtcggc gaagccgcgc gacggctgat cgagagccgt gatcgaccga
     6661 ttgcgatgtc gttactcgat cagcgcgtcg tagccggcct cggcaacgtg tacaaatccg
     6721 aggtgttgtt cttgagcggg ctcaatccgt tcacacccac ctgcgacgtg ccgcgcgaaa
     6781 cgctcgtgcg catgctcgag ctggcacgcc ggtt
//
LOCUS       DATLEE010000534         3555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_621103, whole genome shotgun sequence.
ACCESSION   DATLEE010000534 DATLEE010000000
VERSION     DATLEE010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621103"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VM692_11545"
     CDS             <1..970
                     /locus_tag="VM692_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ52847.1"
                     /translation="YRELLARKGSSDIGFNSLMGYYLTNPSERTRAVSERLQDLGARR
                     IGDMDATGIDLQILSLTSPGVQIFDAATGTALARATNDELAAAIAAHPARYAGLAAIA
                     PQNAAAAAQELERGVRKLNFKGAIVNSHTQGEYLDDEKFWDIFAAAEALDVPVYIHPN
                     TPSNDMIKPFMARGLDGAVFGFAVETGLHALRLVVSGVFDRFPRLKIVLGHLGEGLPF
                     WLFRIDFMHGGSVRSGRYASQPKLKKQASEYLRENFYYTTSGMAWTPPIRYVQEVMGV
                     DRLMYAMDYPYQFVPEEVNVTDELRISYEDHKAFYQTNAERVFKLA"
     gene            complement(980..3232)
                     /locus_tag="VM692_11550"
     CDS             complement(980..3232)
                     /locus_tag="VM692_11550"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HUQ52848.1"
                     /translation="MDRMFVAALAAVLIALATGAAAQQEPAVAAGRVGNLAIPHSAAA
                     IQVDGTLDDAIWKEALALPLTIETYPRENQRPDVETTAYLVENGDQLLIAFDARDPDP
                     SSIRAYLRDRDSAFNDDFVGIVLDTFNDQRRAFEFFVNPFGVQMDLIQDDVSRNESSS
                     WNAIWDSAGEINDRGFTVEIAIPFSQLRFPNVAGEQTWGIDVLRFRPRAQRARISNNP
                     QDRNRNCYLCQFGKFTGFSNAEPGKALEVVPTLTATRTDLRPTMVGPFESGDFESEAG
                     LGLRWGITPDLTLDLALNPDFSQVEADFAQLEENSTFALFYPETRPFFLEGEDYYSSP
                     LQAVFTRTVADPDVGTKFTGRLGTNNTVGVFATNDTVTNLLLPGPFGSQTTSLAQDND
                     AFVGRYTRGFGNTSTIGALVTSREGDGYSNEVAGFDGRYFINDQSTLRFQYLDSRTEY
                     PSTTAAAFGQDEQMDGDAWRMEYRYGSRNWFAQYWHQDLDPTFRADSGFVSRVGIVQD
                     RFEMNRTFYGDQDTWYTDFRLGMQGGRIEGSDGQLINRNLQPFITFQGPMQSFGRLGV
                     GVRKEFWNGQMYDLQGGFMFGQVRPRSGLNFNMEVQRGETIDFTNSRLADQRRVSPQI
                     DWNATQHLLVRLRYTSDRLSAKDGPTVFDAKLMDLRLTWQFNVRSFVRLTLQDQDVER
                     NVDLYANRLLTDPSTSSLATQLLYSYKLNPQTVLFAGYSDNSLEDDTTRELEKTGRTL
                     FFKLSYAWTP"
BASE COUNT          663 a         1128 c         1144 g          620 t
ORIGIN      
        1 gtatcgcgag ctgctcgcgc gcaagggctc gagcgacatc ggcttcaaca gcctcatggg
       61 ctattacctg acgaacccga gcgagcgcac gcgtgcggtc agcgaacggc tgcaagactt
      121 gggtgcgcgg cgcatcggcg acatggacgc gacgggtatc gatctgcaga tcttgtcgct
      181 gacgtcgccc ggcgtgcaga tcttcgacgc cgcgactgga accgcgctcg cacgcgcgac
      241 gaacgacgag ctggcggcgg cgatcgcggc gcacccggct cgctacgcgg gacttgcggc
      301 gatcgcgccg cagaatgcag ctgcggccgc gcaagagctc gagcgcggag tgcgcaagct
      361 gaatttcaaa ggcgcgatcg tcaattcgca tacgcaaggc gagtacctcg acgacgaaaa
      421 attctgggac atcttcgccg cggccgaggc gctcgacgtg cccgtctaca ttcacccgaa
      481 cacgccgtcg aacgacatga tcaagccgtt catggcccgc gggctcgacg gcgccgtgtt
      541 cggcttcgcc gtggaaaccg ggctgcacgc gctgcggctc gtcgtcagcg gcgtgttcga
      601 ccgctttcca cggctcaaaa tcgtgctcgg ccatctcggc gaagggttgc cgttctggct
      661 gttccgcatc gacttcatgc acggcggcag cgtgcgctcg ggccgctacg cgagccagcc
      721 gaagctcaag aagcaagcga gcgagtactt gcgagaaaac ttctactaca cgaccagcgg
      781 catggcgtgg acgccgccga tccgctacgt gcaagaagtc atgggcgtcg atcggctcat
      841 gtacgcgatg gactatccgt atcagttcgt gcccgaggaa gtgaacgtca cggacgagct
      901 gcgcatttcc tacgaggacc acaaggcgtt ctaccagacg aacgccgagc gcgtgttcaa
      961 gctcgcgtag cgccgccggc tacggcgtcc aggcgtagct gagcttgaag aacagcgtgc
     1021 gccccgtctt ctcgagctcg cgcgtggtgt cgtcctcgag gctgttgtcg gagtagccgg
     1081 cgaacagcac cgtctgcggg ttcagcttgt aggagtacag cagctgagtc gccaacgacg
     1141 acgtgctcgg gtccgtgagc aggcgattgg cgtagagatc gacgttgcgc tcgacgtcct
     1201 ggtcctgcag cgtcaagcgc acgaagctac gcacgttgaa ttgccacgtc aggcgcagat
     1261 ccatgagctt cgcgtcgaac accgtcgggc cgtccttggc gctcaaccga tcggacgtgt
     1321 agcgcagccg cacgagcaag tgctgcgtcg cgttccaatc gatctgcggc gagacgcgcc
     1381 gctgatccgc cagccgcgag ttcgtgaaat cgatcgtctc gccgcgttga acctccatgt
     1441 tgaagttcag gccgctgcgg ggccgcacct ggccgaacat gaagccgcct tgcaagtcgt
     1501 acatctgccc gttccagaac tccttgcgga cgccgacgcc gagccgcccg aacgactgca
     1561 tcggcccttg aaacgtgatg aacggctgca ggttgcgatt gatcagttgt ccgtccgaac
     1621 cctcgatacg gccgccctgc atgccgagcc gaaagtccgt gtaccacgtg tcttggtcgc
     1681 cgtaaaaagt acggttcatc tcgaatcggt cttggacgat cccgacgcgc gaaacgaagc
     1741 ccgagtcggc gcggaaggtg ggatcgagat cctggtgcca gtactgggcg aaccagttgc
     1801 gcgacccata gcgatactcc atccgccacg cgtcgccgtc catctgctcg tcctgaccga
     1861 aagccgctgc cgtcgtcgac ggatactccg tccgcgagtc gaggtactgg aagcgcagtg
     1921 tgctctgatc gttgatgaaa tagcggccgt cgaagcccgc gacctcgttg ctgtagccgt
     1981 cgccttcgcg gctcgtcacg agcgcgccga tcgtcgaggt gttgccgaag ccgcgcgtgt
     2041 agcggccgac gaacgcatcg ttgtcctgag caagcgaagt cgtctgcgag ccgaacggcc
     2101 caggcaatag gaggttcgtc acggtgtcgt tggtggcgaa cacgccgacg gtgttgttcg
     2161 tgccgagtcg gcccgtgaat ttcgtcccga cgtcggggtc tgcgacggtg cgcgtgaaca
     2221 cggcttgcag cgggctcgag tagtaatcct cgccctcgag gaaaaacggc cgcgtctccg
     2281 gataaaacag agcgaaggtc gagttctcct cgagctgcgc gaagtcggcc tcgacttgcg
     2341 agaagtcggg attcagcgcg aggtccaggg tcaagtcggg cgtgatgccc catcgcaaac
     2401 cgaggccggc ctcgctctcg aagtcgccgc tctcgaacgg gccgaccatc gtcggccgca
     2461 ggtccgtgcg cgtggccgtc agcgtcggga caacctcgag cgccttgccc ggctccgcgt
     2521 tggagaagcc cgtgaatttg ccgaactggc acagataaca gttgcgattg cggtcttgcg
     2581 ggttgttcga gattcgtgcg cgctgcgccc ggggccggaa acgcagcacg tcgatgcccc
     2641 acgtctgctc gcctgcgacg ttggggaagc gcagctgact gaagggaatc gcaatctcga
     2701 ccgtgaagcc gcggtcgttg atctcgccag ccgagtccca gatcgcattc cacgacgagc
     2761 tctcgttgcg gctgacgtcg tcttggatca aatccatctg cacgccgaac ggattcacga
     2821 agaactcgaa cgcgcgccgt tgatcgttga aggtgtcgag cacgatgccg acgaagtcgt
     2881 cgttgaacgc ggagtcgcgg tcgcgcagat aagcgcgaat agagctcggg tcggggtcgc
     2941 gagcgtcgaa agcgatcagg agttgatcgc cgttctcgac gagatacgcc gtcgtctcga
     3001 cgtcgggccg ctggttctcg cgtggatagg tctcgatcgt cagcggcaac gcgagcgctt
     3061 ctttccagat ggcgtcgtcg agggtgccgt cgacttgaat ggccgccgcc gagtgcggaa
     3121 tcgccagatt gcccacgcga cccgcagcca ccgcgggctc ctgttgagcg gctgctcccg
     3181 ttgccagagc aatcaacacg gctgccaacg ccgccacgaa catacggtcc atgcgctcga
     3241 acgcctcctt ttaccgaccg gacgcgcgcg cccgcgggcc gactgcagcg tcgatgccaa
     3301 cgtcgagcac ggacgtaagc cgctgatttg gtatcgcttt gaggagatcg gaacaacgct
     3361 ccgagtcgcg caacgcagca acaactgccg gtttgacccg cgccttggcg taaaggcgcg
     3421 tgcgatgtac cgcgcgccac gacaaccgag attcggccgt agccaagaga ccccccgaga
     3481 gaaccgcccg gcgtaacacc gccgaaccca gccgttagat gcgaccgccg caaaatagtt
     3541 acacgagcgc gggcg
//
LOCUS       DATLEE010000535         3405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2106554, whole genome shotgun sequence.
ACCESSION   DATLEE010000535 DATLEE010000000
VERSION     DATLEE010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2106554"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VM692_11555"
     CDS             complement(<1..195)
                     /locus_tag="VM692_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52849.1"
                     /translation="MNLVGLWTIVRKESRRVVRIWIQTIVPPAITMTLYFIIFGSLVG
                     GRIGIMDGVSYTTFIAPGLIM"
     gene            complement(246..1172)
                     /locus_tag="VM692_11560"
     CDS             complement(246..1172)
                     /locus_tag="VM692_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ52850.1"
                     /translation="MDALVIKDLRKVYKNGVEALKGIDLTVREGDFYALLGPNGAGKT
                     TAIGIVTSLIRKSGGSVSVFGHDLERDREAAKTCIGVVPQEINLNMFERNDYTLVNQA
                     GYYGVPKNVAEARTEKYLKALHLWDKRTGIARTLSGGMKRRLMIARALVHEPRLLILD
                     EPTAGVDIEIRRSMWDFMREINAKGTTIILTTHYLEEAESLCRNIAIIDEGRIIENDS
                     MANVIGKLQLEVFVLSLLEPLAAAPKLNGYVVRLRNERELEVEVAKGQGLNDVFAQLS
                     AQGLHVSSMRTKTNRLEELFIRLVDKQERRTA"
     gene            complement(1250..1651)
                     /locus_tag="VM692_11565"
     CDS             complement(1250..1651)
                     /locus_tag="VM692_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52851.1"
                     /translation="MTSFPRVLFQLHIGRRWERTDPVAELDEAERELCLLALHAARHA
                     SRQSAYQARLLFGLDAVDVLELGGAPLAALQQLAAVPGTLLCAFRERRWFWEGLFTAT
                     RPEVRRQLTLMALQPAIALAWPPRRPPHASA"
     gene            1929..2504
                     /locus_tag="VM692_11570"
     CDS             1929..2504
                     /locus_tag="VM692_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52852.1"
                     /translation="MRATDDRYRGEQVKFDLARRMIRHEARTGTIRYFTGLNDDRIRK
                     LYTSYFKYGEAPVRRQRGRSPTRIGPLVRTPQRALESGVFANLLLANGLLSVERPPGP
                     PLQNNIELGHRLCECFETFDELVPRSSLSFEWGWNLLTSMRRGDELGIGRCDSCSICY
                     VYDILSLPPSACPACSLFQQRGADAPFAAAG"
     gene            2514..3242
                     /locus_tag="VM692_11575"
     CDS             2514..3242
                     /locus_tag="VM692_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HUQ52853.1"
                     /translation="MCLIVVGVGASARYPLLVAANRDEQHARPTQAAAWWPDAPHVLG
                     GRDLSAGGTWLAIDARGRFAAVTNIREPNRPVGLRSRGSLVADFLTGRESAARYAERA
                     AADGAAFGAFNLLLFDGRELHFASNRSPAANLSTGLHAYSNAPPGTEWPKTASALAAA
                     EPLVSHAAPIAPLFALLAMRDDEGPPESRYQRAHFVVGATYGTRCSTVVLIGTDGRLT
                     FNERTFDAAGTLVGEVRESFALAR"
BASE COUNT          569 a         1108 c         1124 g          604 t
ORIGIN      
        1 catgatgaga cccggcgcga tgaacgtcgt gtacgacacg ccgtccatga tgccgatgcg
       61 cccgccaacg aggctgccga agatgatgaa atacagtgtc atcgtgatcg cgggcggcac
      121 gatcgtctga atccaaatgc gcacgacgcg ccgcgactcc ttgcggacga tcgtccagag
      181 gcccaccaag ttgatgcgcc agagattcgg ctgcgtcacc gtcgcgcgct tcaaagccaa
      241 ctcgttcatg ccgtgcgtcg ctcctgcttg tcgacgagcc gaatgaacag ctcctcgagt
      301 cgattcgtct tcgtcctcat gctcgataca tgcaagccct gcgccgaaag ctgcgcgaac
      361 acgtcgttca atccctggcc tttcgcgacc tcgacttcga gctcgcgctc gttgcgcagg
      421 cggacgacgt agccgttcaa cttcggtgcg gcggcaaggg gctcgagcaa gctcagcacg
      481 aagacctcga gttgcagctt gccgatgacg ttggccatgc tgtcgttctc gatgatgcgg
      541 ccttcatcga tgatcgcgat gttgcggcag aggctctcgg cctcctcgag gtagtgtgtc
      601 gtcaagatga tcgtggtgcc cttcgcgttg atctcgcgca tgaagtccca catcgaccgg
      661 cggatctcga tgtcgacacc ggccgttggc tcatcgagga tcaagaggcg cggctcgtgc
      721 acgagcgctc gcgcgatcat gaggcggcgc ttcatgccgc cggacaacgt tcgcgcgatg
      781 ccggtgcgct tatcccacag gtgcagcgcc ttcaagtatt tctcggtgcg cgcctcggcc
      841 acgttcttcg gcacgccgta gtaaccggct tgattgacga gcgtgtaatc gttgcgctcg
      901 aacatgttca agttgatctc ttgcggcacg acgccgatgc aagtcttcgc cgcctcgcgg
      961 tcgcgctcga gatcgtggcc gaacacgctg accgagccgc cggacttgcg aatcagtgac
     1021 gtgacgatgc cgatggccgt cgtcttgccg gcgccgtttg ggcccaacag cgcatagaaa
     1081 tcgccttcgc gcaccgtgag gtcgatgccc ttcagcgcct cgacaccgtt cttgtagacc
     1141 ttgcgcagat ccttgatgac taacgcgtcc atgaatgccc gtatgacttc cgccggttcc
     1201 gagggcggct attgtacggg ccgcggcgcg cgcgaaaaaa tgcgggtgct taagcgctcg
     1261 cgtgcggtgg tctgcgaggc ggccacgcga gtgcgatagc cggttgcagc gccatcaacg
     1321 tcaactgccg acgcacttcc ggccgagtcg ccgtgaacaa accttcccag aaccaccggc
     1381 gctcccgaaa tgcgcacagc agcgtgccgg gcaccgccgc gagttgctgc agcgcggcaa
     1441 gcggcgcgcc accgagctca aggacgtcga cggcatcgag cccgaatagc agccgcgcct
     1501 ggtaggcact ctgccggctc gcgtggcgcg ccgcgtgcag cgccaagaga cacagctcgc
     1561 gctcggcctc gtcgagctcg gcgaccgggt cggtgcgctc ccagcgccgg ccgatgtgaa
     1621 gttgaaaaag cacgcgtgga aacgaggtca tcgcctcgat cgaggccggc ccggcggtcg
     1681 caagcagcga ctgagcggcg ggcgacagac ccaggcatgg accgcgtcgt tggaccctgg
     1741 tctgcagcaa gccaaggaac gcgcggttca gctcttggac ttggggaagt tggtctaagg
     1801 cgcccggtgt cagcatagcg gtacgacctc ttttcgggtt gtatgtgcca tatatgggcg
     1861 taaatgtcca ctaatggctt ttgtaggata aagtctcgtt tgtggtcgaa gcgtgaggaa
     1921 aggggagcat gagggcgact gacgaccgat atcggggtga gcaggtcaag ttcgacctgg
     1981 ccagacgaat gatccgccac gaggccagga ccggaacgat ccgctatttc acgggcttga
     2041 acgacgaccg tatccgcaag ctctatacga gctatttcaa gtacggcgaa gcgcctgtgc
     2101 gcaggcaacg ggggcgatcg ccgacgcgca ttgggccgct cgtgcggacg ccgcagcgcg
     2161 cgctcgagtc gggagtgttc gcgaatttac tgctcgcgaa cggcttgctg tccgtcgagc
     2221 ggccgcctgg gccaccgctg cagaacaaca tcgagttggg gcaccgcctt tgcgagtgct
     2281 tcgagacgtt cgacgagctc gtcccgcgca gctcgctgtc gttcgaatgg ggttggaacc
     2341 tgttgaccag catgcggcgc ggcgacgagc tcggtatcgg gcgctgcgac tcgtgctcga
     2401 tctgctacgt gtacgacatc ctgtcgttgc cgccgtcggc ttgtcccgcg tgctcgctat
     2461 tccagcagcg cggcgcggac gcgccgttcg cggcagccgg gtagcatggc cgcatgtgct
     2521 tgatcgtcgt cggggttggc gcgtccgcgc gctatccgct gctcgtcgcc gccaatcgcg
     2581 acgagcagca cgcgcggccg acgcaagcgg cggcgtggtg gcccgacgcg ccgcacgtgc
     2641 tcggcggccg cgatctgtcg gcggggggca cctggctcgc gatcgacgct cgcggccggt
     2701 tcgccgctgt cacgaatatt cgcgagccga accggcccgt gggtctgcgc tcgcgcggct
     2761 cgttagtcgc ggactttttg accggccgcg agtccgccgc gcgctatgcc gagcgtgcag
     2821 ccgccgacgg cgcggcgttc ggcgccttca acctgctgct gttcgacggc cgcgagctgc
     2881 acttcgccag taatcgctcg ccggcagcga atctctcaac gggcttgcat gcttacagca
     2941 acgcgccacc gggcacggag tggccgaaga ccgcgagcgc gctggccgcc gccgagccgc
     3001 tggtgtcgca cgccgcgccc atcgcaccgc tgttcgcgct gctcgccatg cgcgatgacg
     3061 aagggccgcc ggagtcgcgt taccaacgcg cgcacttcgt cgtcggcgcg acgtacggca
     3121 cgcgttgctc gacggtcgtg ctcatcggca cggacggccg cctcacgttc aacgagcgca
     3181 cattcgacgc agcggggacg ttggtcggcg aagtgcgcga gagcttcgcg ctcgcgcgct
     3241 agcgaccggc gcccggcaac gcggcgagcc aggcgccgat cgcactgacc tcctcgcgac
     3301 acacggagtg cgccatcgaa taggcgcgcc aatcgacgac gtagcccagc gcctcgagcg
     3361 cgcgtcggga ggtctcgccg agcgcgagcg gcaaaacgtt gtcgg
//
LOCUS       DATLEE010000536         2841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2107281, whole genome shotgun sequence.
ACCESSION   DATLEE010000536 DATLEE010000000
VERSION     DATLEE010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107281"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1007
                     /locus_tag="VM692_11580"
     CDS             126..1007
                     /locus_tag="VM692_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ52854.1"
                     /translation="MIRSKLLPVGLMSVAASGVWSGAAAAPTVQADIQYATTPTGEPL
                     RLDLYTPQSGSGAPLVVWVHGGAWENGNKSAMPLAPVIERGFAVASLDFSPASKAPFP
                     GQVHEIKAAIRFLRAQAKHYGYDASRIAIAGASSGAHLADVVGTSNGVRELEGTLGDH
                     DSESSAVHAIVSYFAATNLTTILAQSTPFGLGVREPALKRLLGAAPKDAEQLARLASP
                     VFQVDRSDPPLLLLHGDQDPQMPINQSHELEGAYEKLGLDAKLIVVHGAAHGGDAFYS
                     PENVQRVVEFLSAQLDR"
     gene            complement(1010..1459)
                     /locus_tag="VM692_11585"
     CDS             complement(1010..1459)
                     /locus_tag="VM692_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ52855.1"
                     /translation="MQTRPPLPPFTAETATIKVKAAEDAWNSRDSERVAGAYTEDSVW
                     RNRDEFVHGRAAIKAFLERKWARELDYRLRKSLWSFTQRRIGVRFQYEWHDAARQWYR
                     SYGNELWEFDDNGLMRRREASINDVAIAENERRFVGASWQTDELPLR"
     gene            complement(1463..1696)
                     /locus_tag="VM692_11590"
     CDS             complement(1463..1696)
                     /locus_tag="VM692_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52856.1"
                     /translation="MSLRDQVDRLLPGWRSWYPSLFDAAADLGLIRARVCPPGSLLLS
                     RRHADIQRAAEEAHREQWGGGPPEDPPDRSHSR"
     gene            complement(1795..2697)
                     /locus_tag="VM692_11595"
     CDS             complement(1795..2697)
                     /locus_tag="VM692_11595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUQ52857.1"
                     /translation="MLALVPLISAGPAVSSIAMVPYAPQYPHQAYGYELASTRLQHNT
                     PAREWLAAADSALLQPEAIALPFAKQAASVASATPLGYAFNVPVGRRVAVTVTSDAEA
                     KGELFVDLFRVTPLRLERVTGALPGRRPQPFVVDVVEGGDYILRVQPPLGIRANIDVG
                     VKTNPLLAFPVEGVGRSAIWSAFGAERDGGRRAHRGVDIFAARGTPVLAATDGWVTRV
                     ETTPVGGNVVWMQPLFGNMRVYYAHLNEQWVEPGEFVLTGQTLGAVGNTGNAITTPPH
                     LHFGVYVRQPGVRGGARDPAEFLR"
BASE COUNT          485 a          962 c          930 g          464 t
ORIGIN      
        1 cagcagctaa ttccaaattt tggaaggggg ctgtgtacag ctgacccccc gaaccacgca
       61 gtacgcgctg cgtgcggccg cttccatgac gtatagtttc ggcggagacc ggggggatcg
      121 ccacaatgat tcgttctaag cttctgcccg ttgggctgat gtccgtagct gcgtccgggg
      181 tgtggtcggg agccgccgcc gcacctacgg tccaagccga cattcaatac gcgacgacgc
      241 cgaccggcga gccgctacgg ctcgacctct acactccgca gtccggctcc ggggcaccgc
      301 tcgtcgtgtg ggtccacggc ggcgcttggg aaaacggcaa caaatcggcg atgccgctcg
      361 cacccgtcat cgaacgaggg ttcgcagtcg cgagcctcga tttcagcccg gcatcgaaag
      421 cgccgtttcc gggccaggtg cacgagatca aggcggccat ccgcttccta cgcgcccagg
      481 cgaagcacta cggctacgac gcgagccgca tcgcgatcgc aggcgcgtcg tcgggcgccc
      541 atctcgccga tgtcgtcggc acgagcaacg gtgttcgcga gctcgaaggg acgctgggcg
      601 accacgactc cgagtcctcg gccgtgcatg ccatcgtgtc gtacttcgcc gccacgaatc
      661 tgacgacgat cctcgcgcag tcgacgccct tcgggctcgg cgttcgagag ccggcgctga
      721 agcgcttgtt gggcgcggcg cccaaggacg cggagcagct cgcgcggctg gcgagccccg
      781 tctttcaagt cgatcgcagc gatccgccct tgttgctgtt gcatggcgac caggatccgc
      841 agatgccaat caatcaatcg cacgagctcg agggcgcgta cgagaaactc gggctcgatg
      901 ccaagctgat cgtcgttcat ggggcggcgc atggcggcga cgcgttctat tctccggaga
      961 acgtgcagcg agtcgtggag ttcttgagcg ctcagctcga ccgttgagct cagcgtaacg
     1021 gcaactcgtc ggtctgccag gaggcgccga caaagcgccg ctcgttctcg gcgatcgcga
     1081 cgtcgttgat gctggcctca cggcgccgca tcaagccgtt atcgtcgaac tcccagagct
     1141 cgttgccgta cgagcgatac cactgcctcg ctgcgtcgtg ccactcgtac tggaagcgca
     1201 caccgatgcg gcgctgcgtg aacgaccaca agctcttgcg taagcggtag tcgagctcgc
     1261 gcgcccactt tcgctcgagg aacgctttga tcgcggcgcg gccatgcacg aactcgtcgc
     1321 gattgcgcca gacggagtct tccgtgtacg cgccggcgac gcgctcggaa tcgcgcgagt
     1381 tccaggcatc ctcggcggcc ttgaccttga tcgtggccgt ctccgcggta aacggcggca
     1441 acggcgggcg agtctgcatc cgtcagcggc tatgcgaacg gtcgggaggg tcctcgggcg
     1501 ggccgccgcc ccactgttcg cgatgagcct cttcggcggc gcgttgaata tccgcatgcc
     1561 gacgactcaa gagcaagctg ccgggcgggc aaacgcgcgc gcgaatcagg cctaagtctg
     1621 cggcggcatc gaacaagctc ggataccacg acctccagcc gggtaggagc cgatcgactt
     1681 gatcacgcaa gctcatggtc ccgagcctag cggacgtcgg cgaagctcgc aacgacgcgt
     1741 gcacggctgc acgcgataga gcaatgcacg agcaattgcc gaacggactg ctcgttaccg
     1801 caagaactcg gcgggatctc gcgccccgcc gcggacgccg ggctggcgca catagacgcc
     1861 gaagtgcaga tgcggcggcg tcgtgatggc attgcccgtg ttgccgacgg ccccgagcgt
     1921 ttggcccgtg agcacgaact cgccgggctc gacccattgc tcgttcaggt gcgcgtagta
     1981 cacgcgcatg ttgccgaaca gcggctgcat ccagacgacg ttgccgccca cgggcgtcgt
     2041 ctcgactcgc gtcacccagc cgtccgtcgc cgcgagcacg ggcgtgccgc gcgcggcgaa
     2101 gatgtctacg ccgcgatgcg cacgccgccc gccgtcgcgc tcggcgccga acgcgctcca
     2161 aatggcgctg cgtccgacgc cctcgaccgg aaacgcgagc agcggattcg ttttgacgcc
     2221 gacgtcgatg tttgcgcgga tcccgagcgg cggttgcaca cgcaggatgt aatcgccacc
     2281 ttcgacgacg tccaccacga acggctgcgg gcggcgcccc ggcaacgctc ccgtgacgcg
     2341 ctcgaggcgc agcggcgtca cgcgaaacag gtctacgaac agctcgccct tcgcctcggc
     2401 gtcgctcgtg acggtcacgg caactctgcg cccgacgggc acgttgaacg catagccgag
     2461 tggcgtcgcg ctcgcaacgc tcgccgcctg cttcgcaaac ggcaaggcta ttgcctcggg
     2521 ttgtagcaat gcggagtcgg ccgcggcgag ccattcgcgc gccggggtgt tgtgctgcaa
     2581 gcgcgtgctc gccagctcgt aaccgtaagc ctggtgcggg tattgcggtg cgtacggcac
     2641 catcgcgatc gagctcacgg cgggcccggc ggagatcagc ggcaccagag ccagcacgcc
     2701 accggtcgcc aaagcgaaaa agttgcgcag cgagcagcag cgccagattg cactcgcggc
     2761 tcgacgcaga acgctttgcc tgcccgctgc actcaccgaa tcgatggtac gcggcgcgct
     2821 tcttcagtgt cgagcgcgcc a
//
LOCUS       DATLEE010000537         2185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2858671, whole genome shotgun sequence.
ACCESSION   DATLEE010000537 DATLEE010000000
VERSION     DATLEE010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2858671"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..929
                     /locus_tag="VM692_11600"
     CDS             96..929
                     /locus_tag="VM692_11600"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ52858.1"
                     /translation="MARRLLKAGISLTVYDTSKEATDDLAKSGAQVASSALEVANNTE
                     VAFLSLPTPDIVQKVCSGLTGAKKMNIVVDCSTTGPGVARIAHAELAKAGIIYMDAPV
                     SGGMAGARDGTLAVMVSGKRDVYDRIEPAIKQFGKLFYCGDGAGLAQVMKLANNIIAA
                     GVIVMSAEVIAMGTKAGLNPRQMCDIINAGSGRNSATQDKFPRSVLPGTFDFGFATGL
                     SYKDVRLCLEEAHAQGVPMPVGAEVFQVLGITKAKYGADSDFTCIAKVYEEWAGVEMR
                     G"
     gene            947..>2185
                     /locus_tag="VM692_11605"
     CDS             947..>2185
                     /locus_tag="VM692_11605"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HUQ52859.1"
                     /translation="MATADRSVAARSAPLAVRMARTLVGYSYARLSPEVVAKAKLCVY
                     DLLSSALAAGELPWSRQSAAIARASSGGAARGAAIIGTSDVVSVHDAAFANGVRSHGL
                     VRDDMHVGSVSHLGTVLVPTLLALTETKNATGKDFLTALVAGYEVGGKVGRMIMDVEV
                     SKIFRPTGTVGPIAAAAAGAKLLGLNEEQTTMALALAANMAAGYNEWAGTGGSEMFFH
                     NGLAARGAVTAVQLAAEGAYASRTALDGDAGMLAAFRRPRPPQVPELFADRPEILAVF
                     FKPVPACNFAQTPAQAAHALAQRHRLRAADIEAVTARVTKAAALYPGCDASGPFEHIL
                     QAKMSIHYNVAAALAHGDFAERNYVPQANPDVQSLAKRVTVEIDAGFTSAFPAKQGAA
                     ILVRTRSGQTFEQSMDNVEPT"
BASE COUNT          378 a          727 c          748 g          332 t
ORIGIN      
        1 gtgccgatcg agatcaattt tcgcaattga acggagtagc aagagtgaag accacggact
       61 acggattcat cggcgtcgga cgcatgggtg cgcacatggc gcgccgattg ttgaaggcgg
      121 gcatcagcct cacggtgtac gacacgtcga aggaagcgac ggacgatctc gccaagagcg
      181 gcgcgcaagt cgcgagctcg gcgctcgagg tcgcgaacaa caccgaggtc gcgttcttga
      241 gcctgccgac gcccgacatc gtgcagaagg tctgctcggg actgacgggg gcgaagaaaa
      301 tgaacatcgt cgtggactgt tcgacgacgg gccccggcgt cgcgcgcatc gcgcacgccg
      361 agctcgcaaa ggccggcatc atttatatgg acgcgcccgt cagcggcggc atggccggcg
      421 cgcgcgacgg cacgctggcc gtcatggtgt ccggtaagcg cgacgtctac gaccgcatcg
      481 agcccgcaat caagcagttc ggcaagctgt tctattgcgg tgacggcgcg ggtctcgcgc
      541 aagtcatgaa gctcgccaac aacatcatcg cggccggcgt catcgtgatg tcggccgagg
      601 tcatcgcgat gggcacgaag gccgggctca acccgcggca aatgtgcgac atcatcaacg
      661 caggcagcgg ccgcaacagc gcgacccagg acaagttccc gcggtccgtg ctgcccggca
      721 cgttcgactt cggcttcgcg acgggcctct cgtataagga tgtccggctg tgcctcgagg
      781 aagcccacgc ccagggcgtg ccgatgcccg tcggcgccga ggtctttcag gtgctcggca
      841 tcacgaaagc gaagtacggc gccgactcgg acttcacgtg tatcgccaag gtctacgagg
      901 aatgggccgg cgtcgagatg cgcggctaat tagaggagta cgccgcgtgg ccaccgccga
      961 tcgatccgtc gccgcccgct ccgcgccgct cgccgtgcgc atggcgcgca cgctcgtggg
     1021 ctattcgtac gcgcgcctgt cgcccgaggt cgtcgcgaag gcgaagctct gcgtttacga
     1081 cttgctgtcg tcggcactcg cggcgggcga gttgccgtgg agccgtcagt cggccgcgat
     1141 tgcgcgggcg agctcgggcg gagcggcgcg cggcgcggcc atcatcggca cgagcgacgt
     1201 cgtgtcggtt cacgatgccg cgtttgcgaa cggcgttcgc agccatggtc tcgtgcgcga
     1261 cgacatgcac gtcggcagcg tcagccattt gggcacggtc ctggtcccga cgttgctggc
     1321 cctgaccgaa accaagaacg cgaccggcaa ggacttcctg acagcgctcg tcgccggcta
     1381 cgaggtcggc ggcaaggtcg gccgcatgat catggacgtc gaggtctcga agattttccg
     1441 gccgacaggc accgtgggcc cgatcgcggc cgccgctgcc ggcgccaagc tgctcggctt
     1501 gaacgaggag cagacgacga tggcgctcgc gctcgccgcg aacatggccg ccggctacaa
     1561 cgaatgggcc ggcaccggtg gcagcgagat gttcttccac aacggcttgg ccgcgcgcgg
     1621 cgccgtgacg gccgtgcagc ttgcggccga aggcgcgtat gcctcgcgca cggcgctcga
     1681 cggcgacgcc ggcatgctcg cggcgttccg ccggccgcgg ccgccgcagg taccggagct
     1741 gttcgcggac cgccccgaaa ttctcgccgt gttcttcaag cccgtgccgg cctgcaactt
     1801 cgcgcagacg ccggcgcagg cggcgcacgc gctcgcgcag cgccatcgcc tgcgcgcggc
     1861 cgacatcgaa gccgtaaccg cgcgcgtgac gaaggccgcc gcactctacc caggctgcga
     1921 cgcgagtggg ccgttcgagc acatcctgca agcgaagatg agcattcact acaacgtcgc
     1981 cgcggcgctc gctcatggcg acttcgccga acgcaactat gtgccgcagg caaaccccga
     2041 cgtgcagtcg cttgcgaagc gcgtgaccgt cgagatcgac gccggcttca cgagcgcatt
     2101 tccggccaag caaggtgcgg ccatcctcgt gcgcacgcgc agcggtcaga cgttcgagca
     2161 gagcatggac aatgtcgagc cgacg
//
LOCUS       DATLEE010000538         4261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8509639, whole genome shotgun sequence.
ACCESSION   DATLEE010000538 DATLEE010000000
VERSION     DATLEE010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8509639"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VM692_11610"
     CDS             complement(<1..386)
                     /locus_tag="VM692_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HUQ52860.1"
                     /translation="MAVAKRIRSAFLTLLAVLGILLWLVALLSFTRVTDPSDDFAQRE
                     TWILLFNSVGIAVLLSLIVINLWQLVRDYRRHVPGSRLRARMVSVLVMVAITPLVGVY
                     IFSVEFINRGIDNWLNVDVEKGLGDAL"
     gene            complement(365..955)
                     /locus_tag="VM692_11615"
     CDS             complement(365..955)
                     /locus_tag="VM692_11615"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HUQ52861.1"
                     /translation="MLCVIAATLPSIGNAQPLVRPEPPVVVDEQNDAGFFEVRSAVAE
                     LRDNVYFLNAVISYRLSTEARDALHSGVPLGIRLDVEIIHPRRWWFDNENAALRQAYQ
                     LEYHALSERYIVLNVNSDDQASFGTLSAALDYLGRVERLPLLDTAVIDDGRGYYVRIR
                     AVLDEEQFPGPLRLLAFWRRDWSIASEWYRWPLRSE"
     gene            complement(1002..2333)
                     /gene="rsmB"
                     /locus_tag="VM692_11620"
     CDS             complement(1002..2333)
                     /gene="rsmB"
                     /locus_tag="VM692_11620"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HUQ52862.1"
                     /translation="MSPEPARAGAKLRAVAAQLVARVLDERVAADELLPTADVVARDR
                     ALLAALVFGALRWHYRLEWQAAQLLKRPLEPAQSALAALLRLGLLQLQELRIPEHAAV
                     SATVDATALLGLRSARGLVNAVLRRFQREREPLARAAQQDEQARFAHPRWLIEAVRAD
                     RPADWQAVLAANNALAPMWLRVNALRTTRTAYLEQLERAGIAATADPDLACAVRLAAP
                     APVDALPGFAAGEVSVQDLSAQRAAELLELAPGQRVLDACAAPGGKTGHILEMLAGRG
                     EVWAVDRDAARLGRVRDNLARLGLEATLVTGDGTSPADWWDGKPFDRILIDAPCSATG
                     VIRRHPDIKVLRRPADVDRAVALQARLLRALWPLLAAGGRLVYATCSVLRPENEAQIA
                     AFRAEEPTIEPAQGVASIQLLPEEARGDGFYYAWLRKTSPLRTPSGLQPQP"
     gene            complement(2323..3261)
                     /gene="fmt"
                     /locus_tag="VM692_11625"
     CDS             complement(2323..3261)
                     /gene="fmt"
                     /locus_tag="VM692_11625"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HUQ52863.1"
                     /translation="MRAPARIAFAGTPDFAVPSLRALVASGATVPLVLTQPDRPAGRG
                     RRLAASPVKQLAVELGLPLAQPVTLRDPSALPPASERPDLMVVIAYGLLLPRTWLEWP
                     RLGCINLHASLLPRWRGAAPIQRALLAGDETTGISVMQMDSGLDTGAVHLTRSTPIRA
                     AETGGNLHDRLAALAAESLLAALPALLAGTSVAVPQRNELATVAPKISKAEGLLDWRA
                     TAVQLERRVRAFNPWPVAEARLDDDRRLRIFEAAAVAGAGEPPGTVLAATRAGIDVAT
                     GAGLLRLKRIQPPSGRVMDVAAYLAAHWLAGAAFVS"
     gene            complement(3279..3827)
                     /gene="def"
                     /locus_tag="VM692_11630"
     CDS             complement(3279..3827)
                     /gene="def"
                     /locus_tag="VM692_11630"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HUQ52864.1"
                     /translation="MARLPVLEYPDPRLRIRAEPVTKFDVELKTLVAAMFETMYAAPG
                     IGLAATQVNVHKRIVVCDIGVDSKQPYCLINPEIIHAEGKTNAEEGCLSVPEFYDFID
                     RAAVIRVRAFDPDGRPFELDAEGMLAVCIQHEMDHLEGKLFVDYLSELKRERLKKKAS
                     KKAKRAAEEAADARARQSVPVI"
     gene            4021..>4261
                     /locus_tag="VM692_11635"
     CDS             4021..>4261
                     /locus_tag="VM692_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HUQ52865.1"
                     /translation="MFFARKPLIRSTTLLAAAAASFILLSSSGAQERDARLQLAAVGQ
                     QPPSPVLNPRHPDSYVVQRGDTLWGIASMFLRDPWY"
BASE COUNT          726 a         1418 c         1469 g          648 t
ORIGIN      
        1 agcgcgtcgc ccaggccctt ctcgacgtcg acgttcagcc agttgtcgat accgcgattg
       61 atgaactcca cggaaaaaat gtagacgccg acgagcggcg tgatcgccac catgacgagc
      121 accgacacca tgcgcgcccg caagcgcgat cccggcacgt gacggcggta gtcgcgcacg
      181 agctgccaca ggttgatgac gatcagcgac agcaacaccg cgatgccgac ggagttgaac
      241 agcaggatcc aggtttcccg ctgcgcgaag tcgtccgagg ggtccgtcac gcgcgtgaac
      301 gagagcagcg caacgagcca cagcaagatc ccgaggacgg cgagcagcgt gaggaatgcg
      361 cttcttattc gcttcgcaac ggccatcgat accactcgct cgcgatcgac caatcgcgcc
      421 gccagaaggc gagcaaccgc aagggcccgg ggaattgttc ttcgtcgagc acggcacgga
      481 tgcgcacgta gtagccgcgg ccgtcgtcga tgacggcggt atcgaggagc ggcaaacgtt
      541 cgacgcggcc gagataatcg agcgcagcac tcagcgtgcc gaacgaggct tgatcgtcgc
      601 tgttgacgtt caacacgatg tagcgctcgc tgagggcgtg gtactcgagc tgatacgcct
      661 ggcgcagcgc ggcgttctcg ttgtcgaacc accagcggcg tgggtgaatg atttccacat
      721 cgagccgaat gccgagcggc acacccgaat gcagcgcgtc gcgcgcctcg gtcgagagcc
      781 ggtaggagat gacggcattc aggaagtaaa cgttgtcgcg caactcggcg acggccgagc
      841 ggacctcgaa gaatcccgcg tcgttttgct cgtcgacgac gacgggcggc tccggtcgaa
      901 cgagcggttg ggcgttgccg atggacggga gcgtggcggc gatgacacaa agcaaggcct
      961 gggcacacga gcgcagctgc gcaagagccg ttccgacttg gctaaggttg cggctggagt
     1021 ccactgggcg ttcgcaacgg agacgtcttc ctcaaccaag cataatagaa gccatcgccg
     1081 cgcgcctcct ccggcaggag ttgtatggac gcgacgccct gcgcgggctc gatcgtcggc
     1141 tcttcggcgc gaaatgcggc gatctgggcc tcgttctccg gtcggaggac cgagcaggtc
     1201 gcatagacca ggcggccgcc cgcggcgagc agcggccaca atgcgcgcaa cagcctcgct
     1261 tggagggcca cggcccggtc gacgtcggct ggccggcgca gcaccttgat gtccggatgg
     1321 cggcgaatga cgcccgtggc actgcacggc gcgtcgatca aaatgcgatc gaacggcttg
     1381 ccgtcccacc aatcggccgg tgacgtgcca tcgccggtta cgagcgtcgc ctcgagaccg
     1441 agccgcgcga ggttgtcgcg cacgcgcccc aagcgagcgg cgtcgcgatc gacggcccag
     1501 acctcgccgc gacccgccag catttcgagg atgtgccccg tcttgccccc gggcgcggcg
     1561 caagcgtcga gcacgcgttg gcccggcgcg agctccagca actcagcggc gcgctgcgcc
     1621 gaaaggtctt gcacggagac ttcgccggcc gcaaagccgg gcagcgcgtc gacgggggcc
     1681 ggcgccgcga gccgcaccgc gcacgcgagg tcgggatcgg ccgtcgccgc gatgccggcg
     1741 cgctcgagct gctcgagata cgccgtacgc gtcgtgcgca gcgcattgac gcgcagccac
     1801 atcggcgcaa gcgcattgtt cgccgcgagc acagcttgcc aatcggcggg acggtcggcg
     1861 cgcacggctt cgatcagcca acgcggatgc gcgaaccgcg cctgctcgtc ttgctgcgcc
     1921 gcacgcgcga gcggctcgcg ttcgcgctga aagcgccgca gcacggcgtt gacgagccca
     1981 cgagcgctgc gcaagcccag caacgccgtg gcatcgacgg tcgcggagac ggcggcgtgc
     2041 tcgggaattc gcagctcctg cagctgcagc aaaccaaggc gcagcagcgc ggccagcgcg
     2101 ctttgcgccg gctccagcgg tctcttgagc agctgcgccg cttgccattc gagccggtag
     2161 tgccaacgca gagcgccgaa caccagcgcc gcgagcaagg ctcggtcgcg cgcgacgacg
     2221 tcggccgtcg gcaagagctc gtcggcggcc acgcgctcgt cgagcacccg tgcgacgagc
     2281 tgggcggcaa cggctcgtag cttcgcgccg gctcgagccg gctcaggaga caaacgccgc
     2341 tcccgccagc cagtgcgccg cgagataggc cgcgacgtcc atgactcgcc ccgacggcgg
     2401 ctgaatccgc ttcaggcgca acagtcccgc gcccgtcgcg acgtcgatgc ctgcgcgcgt
     2461 cgcggccaac accgtacccg gcggctcgcc cgcgccggcg acggccgccg cttcgaagat
     2521 gcgcaggcgc cggtcgtcgt cgaggcgcgc ttcggcgacg ggccacggat tgaacgcgcg
     2581 gactcgccgc tcgagctgca cggccgtcgc gcgccagtcg agcagcccct cggccttgga
     2641 gatcttcgga gcgaccgtcg cgagctcgtt gcgctgcggc acggcgacgg atgtgcccgc
     2701 gagcagcgcg ggcagcgctg cgagcaacga ttctgcggcg agtgcggcaa gccggtcgtg
     2761 cagattgccg ccggtctcgg ccgcgcgaat cggcgtcgag cgcgtgaggt gcacggcgcc
     2821 cgtgtcgagg cccgaatcca tctgcatgac gctgatgccg gtcgtctcgt cgccggcgag
     2881 caaggcgcgt tggatcggcg ccgcgccgcg ccagcggggc agcaacgacg cgtgcagatt
     2941 gatgcacccg aggcgcggcc actcgagcca agtgcgcggc aacagcaaac cataggcgat
     3001 gacgaccatg aggtcgggcc gctcgcttgc gggcggcaac gcgctcgggt cgcgcagcgt
     3061 gaccggctgc gccaacggca gaccaagctc gacggcaagc tgcttcacgg gcgacgccgc
     3121 gaggcgccga ccgcgacccg cgggccggtc aggctgcgtg aggacgagcg gcaccgttgc
     3181 cccgctcgcg acgagggcgc gcaggctcgg gacggcgaag tcgggagttc cggcgaacgc
     3241 gatccgggca ggggcgcgca ccgacgccgg ctctgcggtc agatcacggg cactgattgg
     3301 cgggcgcgtg cgtctgcggc ttcctcggcg gcgcgcttcg ctttcttcga cgccttcttc
     3361 ttcaggcgct cgcgcttgag ctccgagagg tagtcgacga acagcttgcc ctcgaggtgg
     3421 tccatctcgt gctggatgca gaccgccagc attccttcgg cgtcgagctc gaagggcctg
     3481 ccgtcggggt cgaacgcgcg cacgcgaatg acggccgccc gatcgatgaa gtcgtagaac
     3541 tcgggcacag aaagacaacc ttcctcggcg ttagtcttgc cctcggcgtg gatgatttcc
     3601 gggttgatca ggcaataagg ctgcttgctg tcgacgccga tatcgcagac aacaattcgt
     3661 ttgtggacgt tgacctgggt cgcggcgagg ccgatgccgg gcgcggcata catggtctcg
     3721 aacatggcgg cgacgagcgt tttgagctcg acatcgaatt ttgtgaccgg ctccgcgcga
     3781 attcggagtc gagggtccgg atactccagt actgggaggc gtgccattgt ggttcagcct
     3841 tggtttcgtg tataacgcgt caaagttagc ttaacccact gactttgaag ggatagtaag
     3901 gatcgggcct acgccggttt tagccccaaa ggcgcgctcg acgactcggg attatagcag
     3961 tcgagcccag aagggcggtc cttgccactt cgactctttc gggccatagg gagctcacca
     4021 atgttttttg cgcgcaagcc gttgattcga tcgaccactc tgctcgccgc agccgcggcc
     4081 agcttcatct tgttgtcgag ctcgggcgcg caagagcgcg atgcgcggct gcagctcgcg
     4141 gccgtcggcc aacagccacc ttcgccggtc ctgaacccgc gccatcccga cagctatgtc
     4201 gtgcagcgcg gcgacacgct gtggggcatc gcctcgatgt tcctgcgcga tccgtggtac
     4261 t
//
LOCUS       DATLEE010000539         2474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6271250, whole genome shotgun sequence.
ACCESSION   DATLEE010000539 DATLEE010000000
VERSION     DATLEE010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6271250"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VM692_11640"
     CDS             complement(<1..1380)
                     /locus_tag="VM692_11640"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HUQ52866.1"
                     /translation="MHLVMFDIDGTLVDSTGFDSELYATAVDAELGRAVDRDWGSYEH
                     VSDSGILEQLLRDTRSGKERAAIAARVQRRFVELVRDYLARQPGFVREIAGAKRLVER
                     LAALPNVRVAVATGGWEPTARRALRARDLFRRWAVGSTCERRARLRLHRRRRRRPPRR
                     RVRRPARHGGRSGESRARYFLRRARLTRSSSSAKLRLMKTHSRSAFAAVLLAGLLAGT
                     ATAQAPAAPTAQIADAAKALLATLSDAQRDDLTFAFDDEAQRKRWSNLPTGPFTRAGL
                     RMGDLTETQRGAVRALLMAALSSEGYAKVMAIVEADEQLKNSTGRAMFGRDEYYVSFV
                     GEPSATEPWLIQFGGHHLALNITLVGAQGTLVPSHTAAQPAVYELEGKTVRPLGREAE
                     KAFALLQSLDDKQREKAVLGFEIRDLVLGPGRDGQTIQPEGIKGSDLSAAQREQLLGL
                     AQEWTGIVHD"
     gene            complement(1382..>2474)
                     /locus_tag="VM692_11645"
     CDS             complement(1382..>2474)
                     /locus_tag="VM692_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52867.1"
                     /translation="VERARDGGAADVIVDVLGAATELTLVERPANADVEQEKAWLNEQ
                     APGAAKHLALIVVHANAVEPSPGTSNLGSFDLYVPPKLDNRVEIAVQGVIRDAIINAR
                     VDVRGLNREELTRLTQVPRVRSVTVGAEGERDTVLGLNIIVPIAFMSLLIIGVMSAGQ
                     GMLTTTVEEKSNRVIEVLLSAVSPMQLMGGKLVGHMGISLVAMSLYLAVGLIALASFS
                     LFGLLDVSLLFYLLIFFLIAFFTIGSLMMAVGSAVNDMREAQSMMMPLMMTMIVPWIL
                     WMPIVRDPNSTLALVVSFVPPLSSFGMLLRLASSSPPPAWQVWLSIAIGVAGVFGCVW
                     VTAKIFRIGILMFGKPPDLKTLVKWVRAA"
BASE COUNT          426 a          857 c          812 g          379 t
ORIGIN      
        1 gtcgtgcacg atgccggtcc attcctgcgc gaggccgagc agttgctcgc gctgcgccgc
       61 gctcaagtcc gagcccttga ttccctcggg ctgaattgtc tggccgtcgc ggccggggcc
      121 gagcacgagg tcgcggattt cgaacccgag gacggccttc tcgcgttgct tgtcgtcgag
      181 cgactgcagc agtgcgaacg ccttctcggc ttcgcggccg agcggccgca cggtcttgcc
      241 ctcgagctcg tagacggcgg gttgcgccgc cgtatggctg gggaccagcg tgccctgggc
      301 gcccacgagc gtgatgttca gcgccaagtg gtgcccgccg aactggatca accacggctc
      361 agtcgcagac ggttcgccga cgaacgacac gtagtattcg tcgcgcccga acatcgcgcg
      421 gcccgtcgag ttcttcagct gctcgtcggc ctcgacgatc gccatgacct tggcgtagcc
      481 ctcgctgctc aacgcggcca tcagcagcgc gcgcacggcg ccgcgctgcg tttccgtgag
      541 gtcgcccatg cgtaaacccg cacgcgtgaa cgggcctgtc ggcagattcg accagcgctt
      601 gcgctgcgct tcgtcgtcga acgcgaaagt caggtcgtcg cgctgagcgt cgctgagcgt
      661 ggcgagcagc gccttggcgg cgtcggcgat ctgcgccgtg ggagccgccg gcgcttgcgc
      721 ggtcgcagtg cccgcgagca gtcccgcgag caacactgcc gcgaacgcgg aacgcgaatg
      781 cgttttcatg agcctgagct tggcgcttga gctcgagcgc gtcaatcgcg cgcggcggag
      841 gaagtaccga gctcgagact cgccagaacg gcctccgtgt cgcgcaggtc tgcgaacgcg
      901 acgtcgtggc ggacggcgcc gccgacggcg atgaagccgt agccgagctc ggcgctcgca
      961 cgttgatccc acggcccatc gccgaaatag gtcgcgcgca cgcagcgcgc gccgcgccgt
     1021 cggctcccac ccgcccgtcg ccacggccac gcggacgttc ggcagcgccg ccaatcgctc
     1081 gacgagcctt ttcgcgccgg caatctcgcg aacgaaaccc ggctggcgcg ccaggtagtc
     1141 gcgcacgagc tcgacgaaac ggcgctgcac gcgcgcggcg atcgcggctc gctccttgcc
     1201 gctgcgcgtg tcgcgcagta gctgctcgag gatgccgctg tcgctgacgt gctcgtacga
     1261 gccccaatcg cgatcgacag cgcgcccgag ctcggcgtcc acggccgtgg catagagctc
     1321 cgagtcgaag ccggtcgaat cgacgagcgt gccgtcgatg tcgaacatca cgagatgcac
     1381 gctaagccgc tcgcacccac ttcacgagcg ttttcaaatc cggcggcttg ccgaacatca
     1441 agatgccgat gcggaagatc tttgccgtga cccagacgca gccgaacact ccggccacgc
     1501 cgatcgcaat cgacagccag acctgccaag ccggcggcgg gctcgacgac gcgagccgca
     1561 gcagcatgcc gaacgacgag agcggcggca cgaagctcac gacgagcgcg agcgtcgagt
     1621 tcgggtcgcg gacgatcggc atccacaaaa tccacggcac gatcatcgtc atcatcagcg
     1681 gcatcatcat cgattgcgct tcgcgcatgt cgttgacggc cgacccgacg gccatcatca
     1741 gcgagccgat cgtgaaaaac gcgatcaaga aaaagatcag gagatagaac agcagcgata
     1801 cgtcgagcaa gccgaacaac gagaacgacg cgagcgcgat gagcccgacc gccagataca
     1861 ggctcatggc aacgaggctg attcccatgt ggccgacgag cttgccgccc atgagctgca
     1921 tcggcgacac ggccgacagc agcacctcga tgacgcgatt cgacttctcc tcgaccgtcg
     1981 tcgtcagcat gccctgcccc gcggacatca cgccgatgat cagcaagctc atgaacgcga
     2041 tcggcacgat gatgttgagc ccgagcacgg tgtcgcgctc gccttcggcc ccgaccgtga
     2101 ccgagcgcac gcgcggcacc tgcgtgaggc gcgtgagctc ctcgcggttc aggccgcgca
     2161 cgtcgacgcg ggcattgatg atggcgtcgc gaatcacgcc ttgcacggcg atctcgacac
     2221 gattgtcgag cttcggcggc acatagagat cgaagctacc gaggttcgag gtgcccggcg
     2281 acggctcgac ggcgttcgca tgcacgacga tcagcgcgag atgcttggcg gcgccgggcg
     2341 cctgctcgtt gagccacgcc ttctcttgct cgacgtcggc gtttgcgggc cgctcgacga
     2401 gcgtcagctc cgtggccgcg ccgagcacgt cgacgatgac gtccgcggcg ccgccgtcgc
     2461 gcgcgcgctc gacg
//
LOCUS       DATLEE010000540         3226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10752808, whole genome shotgun sequence.
ACCESSION   DATLEE010000540 DATLEE010000000
VERSION     DATLEE010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10752808"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VM692_11650"
     CDS             complement(<1..358)
                     /locus_tag="VM692_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUQ52868.1"
                     /translation="MQRRKLGNLEVSALGLGCMGLSFGYGPAWDRNESIKLLRAAVER
                     GVTFFDTAEIYGPFTNEELVGEALEPFKGRVVIATKVGGQIGPGKGPMDLDSRPEHIK
                     EVAEASLKRLRVDAIDL"
     gene            442..2043
                     /locus_tag="VM692_11655"
     CDS             442..2043
                     /locus_tag="VM692_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HUQ52869.1"
                     /translation="MSLLTAADWLVIAVYFIVLYAVVLRHSKTNRQNPTEFFLAGRNA
                     GWFIVGASIFATNIGSEHLVGLAGSGASGQFPAAQFELIAAFALLLLGWLFVPFYLRS
                     GVFTMPEFLERRYSSGVRFYLAAISIVGYVLTKISVTIAAGAIVFEALMGIDFWTGAV
                     IVVIATGVYTVIGGMRAVLVSDMFQMFVMIGGAIVVTVVGLNAVGGWNELRAAAPPEF
                     FALWRATSDPDFPWTGILFGAPILAIWYWCTDQYIVQRTLAAKSISEARRSTLFAGLL
                     KQLPLLIFVVPGIIAYVLAQRGQLPLADPDSALPTLIGSLLPAGLRGIVVAGLLAALM
                     SSLSSVFNSCATLVTMDVYQRLAPHHSERRLVLVGQLATVVMVLFGLAWIPLMDVISG
                     QLYVYLQSVQAYISPPIAAVFLIGVLWPRANARGALAALLVGLVLGVGRLVLEVDEVV
                     FTGWLGSLVEMNFLHFAVLLFVVCSAVLVLVSLSTQPPDAARVAPLTVHHGRDPALRD
                     PSRATDVALSIALVALVAAAWLYFS"
     gene            2235..>3226
                     /locus_tag="VM692_11660"
     CDS             2235..>3226
                     /locus_tag="VM692_11660"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HUQ52870.1"
                     /translation="MRLDQGQQRERMVERQLARRGIRDPGVMRAMRKVPRELFVPADL
                     AEFAYDDTPLPIAANQTISQPYIVALMVDALALEPSDKALEIGTGSGYAAAVLAELAR
                     EVFTIERQLTLCTLARSRLQRLGYRNIEVRCADGTTGWPEQAPFDAIAVTAGGPVVPQ
                     TLRAQLAVGGRLVIPVGDEGVQRLLRVWRTDDTTFEQEDLGAVRFVPLIGVEGWDDDA
                     LGERRPTNGSRRRLPARPSGAAATDRAAARPRTIPEIVARAAEPFEHPRDVELAPLLA
                     RIGRARVMLLGEATPGTAEFYDLRARITLELMRADPDVRIVAVEADWPDARQIHR"
BASE COUNT          459 a         1111 c         1047 g          609 t
ORIGIN      
        1 acagatcgat cgcgtcgacg cgcagccgct tcagcgatgc ctcggcgact tccttgatgt
       61 gctcgggccg gctgtcgaga tccataggcc ctttgccggg gccgatctgg ccgccgactt
      121 tcgtcgcgat gacgacccgc cctttgaacg gctcgagggc ctcgccgacg agctcctcgt
      181 tcgtgaacgg gccgtagatc tcggccgtgt cgaagaacgt gacgccgcgc tcgacggccg
      241 cgcgcagcag cttgatgctt tcgttgcgat cccacgcggg cccgtagccg aacgacaggc
      301 ccatgcagcc caaacccagc gcggagactt ccaagttgcc gagcttacgt cgttgcatct
      361 gccgtcctcg ttgcggcgcg ccgcatccgc ggtcacgata gaccgcagct agcgccttag
      421 tcgagcaagg aaggaataat aatgagcctg ctcacggccg ccgactggct cgtcatcgcg
      481 gtttacttca tcgtgctgta tgccgtcgtg ctgcgccaca gcaaaacgaa ccggcagaac
      541 ccgacggagt tcttcctcgc gggccgcaac gccggctggt tcatcgtcgg cgcctcgatc
      601 ttcgcgacca acatcggttc cgagcacctc gtcggcctcg cgggctcggg cgcgtcggga
      661 caattcccgg ccgcacagtt cgagctgatc gccgcattcg ccctgctgct gctgggctgg
      721 ctgttcgtgc cgttctactt gcgctcgggc gtcttcacga tgccagagtt cctcgagcgg
      781 cgttactcgt cgggcgtgcg cttctacctc gccgcgatct cgatcgtcgg ctacgtgctg
      841 acgaagatct ccgtgacgat cgcggccggc gccatcgtgt tcgaggcgct gatgggcatc
      901 gacttctgga ccggagccgt catcgtcgtg atcgcgaccg gcgtctacac ggtcatcggc
      961 ggcatgcgcg ccgtgctcgt gtccgacatg ttccaaatgt tcgtcatgat tggcggcgca
     1021 atcgtcgtga ccgtcgtcgg attgaacgcg gtcggcggct ggaacgagtt gcgcgccgca
     1081 gcgccacccg agttcttcgc tctttggcgc gcgacgtcgg accccgactt cccgtggacc
     1141 ggcattctgt tcggcgcgcc gatcctcgca atctggtact ggtgcacgga ccaatacatc
     1201 gtgcagcgca cgctggccgc gaagtcgatc agcgaggctc gccgctcgac cttgttcgcc
     1261 ggcctcttga agcagctgcc gttgctgatc ttcgtcgtgc ccggaatcat tgcctacgtg
     1321 cttgcgcagc gcggccagtt gccgctcgcg gaccccgaca gcgccttgcc gaccttgatc
     1381 ggctcgctac taccggcagg gttgcgcggc atcgtcgtcg cgggtctgct cgccgcgctg
     1441 atgagctcgt tgtcgtcggt cttcaactcg tgcgcgacgc tcgtgacgat ggacgtctac
     1501 cagcggcttg cgccgcacca ctccgaacgg cggctcgtgc ttgttggcca gctggctacg
     1561 gtggtcatgg tgctgttcgg cttggcctgg atccctctga tggacgtgat ctcgggccag
     1621 ctctatgtct atctgcaaag cgtgcaggcc tacatctcgc cgccgatcgc ggccgtcttc
     1681 ctgatcggcg tgctgtggcc gcgcgccaac gcgcgcggtg cgctcgcggc gctgctcgtg
     1741 gggctcgtgc tcggcgtcgg caggctcgtg ctcgaggtcg acgaggtggt gttcaccggc
     1801 tggctcggct cgctcgtcga gatgaacttc ttgcacttcg ccgtgctgct gttcgtcgtc
     1861 tgttccgccg tgctcgtgct cgtaagcttg tcgacgcagc cgccggacgc ggcgcgcgtt
     1921 gcaccgctga cggttcacca tggccgggac cccgcgttgc gggacccgtc gcgcgcaacc
     1981 gacgttgcgc tcagcatcgc gctcgtggcg ctcgtcgcag ccgcgtggct ctatttctcc
     2041 tgaccgctgc cgcaactgac gagcgcatta caaaattttc aaaggtgctt gcgccgatga
     2101 gttagcgcaa ctcctcgctc gcctgcgcag aattgcgcgg gtgccggccg cggagttcca
     2161 tggcatgcga cttgcttgcg cagaaccttt cgatcaccag gacatcggcg gccagaccgc
     2221 agctaggcga agggatgcgc ttagaccagg gacaacagcg cgaacgaatg gtcgagcgcc
     2281 agctcgcgcg gcgcggcata cgcgaccccg gcgtgatgcg cgccatgcgc aaggtaccgc
     2341 gagagctgtt cgtgcccgcg gaccttgcgg agtttgctta cgacgacact ccgttgccga
     2401 tcgcggcgaa tcagacgatc tcccagccgt acatcgtcgc gctcatggtc gacgcactcg
     2461 cgctcgagcc cagcgacaaa gcgctcgaga tcggcacggg ctccggttac gcggcggccg
     2521 tgctcgcaga gctcgcccgc gaagtgttca cgatcgaacg gcagctcacg ctctgcacgt
     2581 tggcccgctc gcgcctgcag aggctcggct atcggaacat cgaggtccgc tgcgccgacg
     2641 gcacgacggg ctggcccgag caagcgccgt tcgacgcgat cgccgtgaca gccggcggcc
     2701 cggtagtgcc ccaaacgctg cgcgcgcaac ttgccgtcgg gggccggctc gtgattcccg
     2761 tcggtgacga gggcgtgcaa cgcctgttgc gggtctggcg caccgacgac acgaccttcg
     2821 agcaagaaga cctgggcgcc gtccgcttcg tgccgctgat cggcgtcgaa ggctgggacg
     2881 acgacgcact cggcgagcgt cgcccgacga acggctctcg gcgtcgcctg cccgcgcggc
     2941 cgtcgggcgc tgccgcaacg gatcgcgcgg ccgctcgtcc gcgcacgatt ccggagatcg
     3001 tcgcgcgcgc ggccgagccg ttcgagcatc cgcgcgacgt cgagctcgct ccattgctcg
     3061 cgcgcatcgg ccgcgcccgc gtgatgctgc taggtgaagc cacgcccggc actgccgagt
     3121 tctacgatct gcgcgcgcgc atcacgctcg agctcatgag agccgacccg gacgttcgca
     3181 tcgttgccgt cgaggccgat tggcccgacg cccggcagat ccatcg
//
LOCUS       DATLEE010000541         3329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10003712, whole genome shotgun sequence.
ACCESSION   DATLEE010000541 DATLEE010000000
VERSION     DATLEE010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10003712"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VM692_11665"
     CDS             <1..360
                     /locus_tag="VM692_11665"
                     /inference="COORDINATES: protein motif:HMM:NF028211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HUQ52871.1"
                     /translation="VLLMVLVMHLDYRKLREPWLVYALLLGSLALLVAVLFAAPLLQS
                     EVLTAELVYALTVEWAQSLEDVLQRRCMAGLAADFGLRTAPAAAAALEQLGIWDAARA
                     ARELADYRELAARHGAR"
     gene            complement(357..584)
                     /locus_tag="VM692_11670"
     CDS             complement(357..584)
                     /locus_tag="VM692_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52872.1"
                     /translation="MQIRSLAIASLALLAASAALAGESAPKVAQPPAAPRQTLVAEVT
                     AGMRELLRAVTPEISLPALEVKLPTLDAQRR"
     gene            complement(705..953)
                     /locus_tag="VM692_11675"
     CDS             complement(705..953)
                     /locus_tag="VM692_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52873.1"
                     /translation="MDICHDHNLNKPLDVPKPYGVRVSIKPSDTFARLLGTGWQRHHW
                     YATREERDAALADMASEHLYSRQGDKPTLTYEAVEQKA"
     gene            complement(1014..1709)
                     /locus_tag="VM692_11680"
     CDS             complement(1014..1709)
                     /locus_tag="VM692_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HUQ52874.1"
                     /translation="MTTRNLQSDALGTTRLVQAGDGLVIERDTRTAHALVRWLGRRLA
                     AREAAALVALAGVEHVPRLLEFDGAVVRRTFLPGVPLHEAPPRSRAYFVRALRLLRLV
                     HGAGIAHNDLAKEANWLVAPGEHCAIVDFQLATLGTPRRGTTLGSPRRGTRSAARTAG
                     FRRRAYEDLRHLLKHKRTYQPQALTARQRRLLARPTAPTRVWRALVKPLYLFTTRTLL
                     GWPDRAGPAERRS"
     gene            1797..>3329
                     /locus_tag="VM692_11685"
     CDS             1797..>3329
                     /locus_tag="VM692_11685"
                     /inference="COORDINATES: protein motif:HMM:NF025869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HUQ52875.1"
                     /translation="MQPERPCYVERAADRRLDEALRAKRLCCVLGAHGIGKSSLLLRA
                     ARSLRAAGTLVAKVDLRRMAELGEGDTEGDWLRRVAQRVAEQLELGVDAGQWWDAHDV
                     LGENRLVSFFWEVVLTNTTAPVVVLVDDVDVVLERSLGADFLEAIGECYERRERERDF
                     ARLSFALAGCASQRALAGASGDTPFAKAEVIEPADFTAEQSYRLAVAFGGQQELAQAL
                     MDRICVWTGGQPYLTQRVARSVARKGGRLEDVERVVREQLLAPGAADRDPLLSQVRVW
                     LGEPSRPARRATRLLQKLSAGGKVAQPAEPAVWERLWLAGVASVDDANQLRVRNRVIK
                     ELVAARWLNPASSGWRWAAAAAVLLAALAAGGYWYTQRLPVGDIDTLTSATAEPSAVE
                     DAYRRLRNLPGFAERADELWLEALGRQSRAATTLSSAMAADTRMRELPEQDTAADGLL
                     GEFWLRRAREQAHAEQRGAALLLAQRAAQLPAAEPAAAAYLSELVGDDYARLERSLRI
                     AGT"
BASE COUNT          461 a         1111 c         1230 g          527 t
ORIGIN      
        1 gtgctgctga tggtgctggt gatgcacctc gactaccgca agctgcgcga gccctggctg
       61 gtctacgcgc tgctgctcgg ctcgctggcg ctgctcgtcg ccgtgctctt cgcggcgccg
      121 ctgctgcaga gcgaagtgtt aacggccgag ctcgtctacg ccctgacggt cgagtgggcg
      181 cagtcgctcg aagacgtgct gcagcggcgt tgcatggccg ggctcgccgc cgacttcggg
      241 ttgcggaccg cgccggccgc ggctgcggcg ctcgagcagc tcggcatttg ggatgccgcc
      301 cgcgccgcgc gcgagctcgc cgactatcgc gagctcgctg cgcgccacgg cgcgcgttag
      361 cgccgctgcg cgtcgagcgt cggtagcttc acttcgagcg ccggcagcga aatctccggt
      421 gtaacggcgc gcaacagctc gcgcattcca gccgtgactt cggcgaccag tgtctgccgc
      481 ggcgccgccg gcggctgtgc gaccttcggc gcagactcgc cggcgagcgc cgccgaggct
      541 gcgagcaacg cgaggcttgc gatggcgagg gagcgaatct gcatgaccgt aactccgttt
      601 gggggggtct cacggagtaa taacgaacga gcgcggctgc gattacagcc ggcacgtcaa
      661 gaacggtcga gaaccgcgaa gatggctgta gcgcgcctgg acggtcaggc cttctgctcg
      721 acggcttcgt acgtcagcgt cggcttgtcg ccttggcgcg aatagagatg ctcgctggcc
      781 atgtcggcca gggccgcatc gcgctcctcg cgcgtcgcgt accagtggtg ccgttgccag
      841 cccgtgccga gcaagcgcgc gaacgtgtcg ctgggcttga tgctgacgcg gacgccgtaa
      901 ggcttcggca cgtcgagcgg cttgttcaag ttgtggtcgt ggcagatgtc catgcgatca
      961 gtgttgcgag cgagagggcg gcattttagc cgacgcgccg cacggcggcg accttagctg
     1021 cgtcgctcgg ccggcccggc tcggtcgggc cacccgagca gcgtgcgcgt cgtgaacaga
     1081 tagagaggtt tgacgagcgc gcgccagacg cgcgtcggcg ccgtcggccg cgccagcaag
     1141 cggcgctggc gagccgtcaa cgcctgcggc tgatacgtgc gcttgtgctt cagcagatgg
     1201 cgcaggtctt catacgcgcg cctgcgaaag ccggccgtcc gggccgcaga ccgcgtcccc
     1261 cttcggggac ttccaagggt cgtccccctt cggggagttc caagcgtcgc gagttggaag
     1321 tcgacgatcg cacagtgctc gccgggcgcg acgagccagt tcgcttcctt cgctagatcg
     1381 ttgtgcgcga tgccggcgcc atggacgagg cgtagcaggc gcagcgcgcg aacgaagtag
     1441 gcgcgcgagc gtggcggcgc ttcgtgcagc ggcacgcccg gcaagaaggt gcggcgcacg
     1501 acggcgccgt cgaactcgag cagccgcggt acgtgctcga cgccggcgag cgcgaccaaa
     1561 gctgccgcct cgcgagcggc caagcgccgg ccgagccaac gaaccaacgc gtgtgccgtc
     1621 cgcgtgtcgc gttctatgac caagccgtcg ccggcctgca cgagccgcgt ggtgcctaaa
     1681 gcgtcgctct ggagattcct tgtcgtcatg cgtcggacgg atcgtgcggc gctgtacaat
     1741 cgcgccgcat gcctagtgtg acccagcccg cggagttttt cgtggtcgga ggcccggtgc
     1801 aaccggagcg cccgtgctac gtggaacgcg cggcggatcg ccggctcgac gaggcgctgc
     1861 gcgccaagcg tttgtgctgc gtgctgggtg cgcacggtat cggcaagtcg agcctgttgc
     1921 tgcgcgctgc gcggagcttg cgcgccgccg gaacgctcgt cgccaaggtc gaccttcgtc
     1981 gcatggccga gctcggcgaa ggcgataccg agggcgattg gttgcggcgt gtcgcgcagc
     2041 gtgtcgccga gcagctcgag ctcggcgtcg acgccggcca atggtgggat gcgcacgacg
     2101 tgctcggcga gaaccgactc gtctcgttct tctgggaggt cgtgctcacg aacacgacgg
     2161 cgcccgtcgt cgtgctagtc gacgacgtcg acgtggtgct cgagcgtagt ctcggggccg
     2221 actttctcga ggccatcggc gagtgttacg agcgccgcga gcgcgagcgc gatttcgcgc
     2281 gcttgagctt cgcactcgca ggttgcgcgt cgcagcgcgc gctcgccggc gcaagcggcg
     2341 atactccgtt cgcgaaagcc gaggtgatcg agcccgccga cttcacggcc gagcagtcgt
     2401 atcgcctcgc tgtcgcattc ggcggtcagc aagagcttgc tcaagcgctg atggaccgca
     2461 tctgcgtgtg gaccggcggt cagccgtacc tgacgcagcg cgtcgcgcgc agcgtcgcgc
     2521 gcaaaggcgg acggctcgag gatgtcgagc gcgtcgttcg cgagcagctg ttggcgcccg
     2581 gcgcggccga ccgcgatccg ctgctgagcc aggtgcgcgt ctggctcggc gagccgtcgc
     2641 ggcctgcacg gcgcgcaacg aggctgctgc agaagttgag cgccggcggc aaggtcgcgc
     2701 agccggcgga gcccgccgtc tgggagcggc tgtggctggc gggcgtcgcg agcgtcgacg
     2761 acgcgaacca gctgcgggtg cgcaatcgcg tcatcaagga gctcgtggcc gcgcgctggc
     2821 tgaaccccgc gagcagcggc tggcgctggg ccgcagcggc cgcggtcctg ctcgccgcgc
     2881 tcgcggccgg cggctactgg tacacgcaac gcctgcccgt aggcgatatc gatacgttga
     2941 cgagcgcgac cgccgagccg agcgccgtcg aggatgccta tcgtcgcctg cgtaacttgc
     3001 cgggtttcgc tgagcgcgcg gacgagctct ggctcgaggc gctcggcagg cagagccgcg
     3061 ccgctacgac gctgtcgtcc gccatggcgg ccgacactcg catgcgcgag ctgccggagc
     3121 aagacacggc ggcggacgga ctgctcggcg agttctggtt gcgccgcgcg cgcgagcagg
     3181 cgcacgcgga gcagcgcggc gcggcgctgc tgctcgcgca gcgtgccgca cagttgccgg
     3241 cggcagagcc tgccgccgcg gcgtatctct ccgagctcgt cggcgacgac tacgcgcggc
     3301 tcgagcgttc gctgcgcatc gcgggcacg
//
LOCUS       DATLEE010000542         3326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8511693, whole genome shotgun sequence.
ACCESSION   DATLEE010000542 DATLEE010000000
VERSION     DATLEE010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8511693"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VM692_11690"
     CDS             complement(<1..978)
                     /locus_tag="VM692_11690"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ52876.1"
                     /translation="MKASFRTIGLAAAALATVGVLAPAAAQQGRPQGAAPAAGAQAAP
                     AGGQAARPAPPALGDGPWDLQTETGMVHVSVLTKDLQSPWGLAFLPDGGMLVTERPGR
                     LRVVRDGKLDPKPIDGLPDLVATGISGLFGIALHPDFARNRLVYLAYPKPQGADRDSL
                     TLAVARARWDGGPALADVKDIFVAKDYYSTAMSRENNRCCGQGPSNGSFGARLEFDKQ
                     NRLYITSGDRNWGEKSQDPQSHIGKIVRVNDDGSVPKDNPFVGKKDYLPEIYTMGHRN
                     QTGLRFDPTTGTLWSTEFGPAGGDELNRIEAGKNYGWLLITHGNHYNNEP"
     gene            1128..2135
                     /locus_tag="VM692_11695"
     CDS             1128..2135
                     /locus_tag="VM692_11695"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ52877.1"
                     /translation="MRLIAAVPLVAALAASSAPAFAHHSVSMFDTSQEILIEGTVARF
                     DWVNPHMYLVVETKGPDGKPALVEGEGLGITQALVDGLDRDALKPGTRVVVRANPNRG
                     GWGKQVRIQDVTTEDGEIHPFYAANRRTRVLTPAESIEGHWAPARAALGAAFGAMAQW
                     PVTPAGRAAQAGLAADGLCFTEPTPVLSILDELRTVEIGDQEVVLRFDNTGDHVDRIV
                     HMGAQHPANVQPSRHGHSIGRWEGKTLVIDTIGFEPSGSGIALNVPSSASKHTVERLT
                     LTEDRLRLRYEVTVEDPTYLSAPATLTQQWDHRPDLEFSQDTGTCDPEVANRYKDSVP
                     K"
     gene            2168..3283
                     /locus_tag="VM692_11700"
     CDS             2168..3283
                     /locus_tag="VM692_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52878.1"
                     /translation="MATRLHGSLGSIAALIGATVLGSYPAASQQAGAAPPLVITAFGE
                     QPAPAYTVGRTSWGEPVLQGVWSSDDTAGIPLQRQEQFGDRRYLSEQEYAERSGQVER
                     GVERAENEATSSFRQDFARRAFWQTSLIVDPPDGRMPAYTAEGMKRPMPRGTYGNGPL
                     DSYTDFSTYERCITRGIAGSSLRVVYGNGMRIVQAPGVVAISYEMIHDTRLLYTDGRP
                     HLSQAIRQYLGDSRAHWEGDELVVETTNLTDRTAIGLNGNGNRHSSEMKLTERFKRVA
                     DDVIQYQTTVDDPVTYERPFTMSYPLTPLEGGKLLPYDCHEGNYALLQSLGAERAEDR
                     ALAADKAKGINRPRRPVQDGLGVGGQPISEGAGIPTR"
BASE COUNT          570 a         1131 c         1080 g          545 t
ORIGIN      
        1 cggctcgttg ttgtaatggt tgccatgcgt gatcaaaagc cagccgtagt tcttgccggc
       61 ttcgatgcga ttgagctcgt cgccgccggc cggaccgaac tccgtcgacc acagcgtgcc
      121 ggtcgtggga tcgaagcgca gccccgtctg gttgcgatgc cccatcgtgt agatctcggg
      181 cagataatcc ttcttaccga cgaacggatt gtctttggga accgagccgt catcgttcac
      241 gcgcacgatc ttgccgatgt gcgactgggg atcctgcgat ttctcgcccc agttgcggtc
      301 gcccgacgtg atgtagagcc gattctgctt gtcgaactcg aggcgcgcgc cgaagctgcc
      361 gttcgacggc ccctggccgc agcagcggtt gttctcgcgg ctcatcgccg tgctgtagta
      421 gtccttcgcc acgaagatgt ctttgacgtc ggcgagcgcg gggccgccgt cccagcgcgc
      481 gcgtgcgacc gccagcgtca acgaatcgcg gtcggcccct tgcggcttcg gatacgcaag
      541 gtagacgagc cggttgcgcg cgaaatcggg gtgcagcgcg atcccgaaca aaccgctgat
      601 gcccgtggcg acgaggtccg gcagcccgtc gatcggcttt ggatcgagct taccgtcgcg
      661 gaccacgcgc agccgtccag ggcgctcggt cacgagcatc ccgccgtcgg gcaggaaggc
      721 gagaccccac ggcgactgca ggtccttggt cagcaccgat acgtggacca tgcccgtctc
      781 ggtctgcaaa tcccaaggtc cgtcgcccaa cgcaggtggt gcggggcgtg ctgcctgccc
      841 tcccgcggga gcggcttgtg cacccgcagc cggagcggcc ccctgcggcc gtccttgttg
      901 agccgcggcc ggagccaaaa cgccgacggt cgcgagcgct gcagccgcaa ggccgatcgt
      961 gcggaacgat gctttcacgg acgcttctcc ttattggttt gcgcgatact agcgccaacc
     1021 atccctcgag gtaacgggcg gcgggcggcc gttatcaatt cataaggtgc agtccgcccg
     1081 gcgatctctc tatcattgtc cccactcgtt ccccctgagg ttcgcaaatg agactgatcg
     1141 ctgctgttcc gctcgtcgcc gcgttggccg cctcgagcgc ccccgcgttt gcccaccaca
     1201 gcgtgtccat gttcgacacg tcgcaagaga tcctcatcga gggcacggtc gcgcggttcg
     1261 actgggtcaa cccgcacatg tacctggtcg tcgagaccaa gggaccggac ggtaagccgg
     1321 cgctcgtcga aggcgaaggc ctcgggatta cgcaagcgct cgtcgacggc ctcgaccgcg
     1381 acgccctgaa gcccggcacc cgcgtcgtcg ttcgcgccaa tcccaatcgc ggcggctggg
     1441 gcaagcaggt ccgtattcaa gacgtcacga cggaggacgg cgaaatccat ccgttctatg
     1501 ccgcgaatcg tcggactcgc gtgctgacgc cggccgaaag catcgagggc cattgggccc
     1561 cggcgcgtgc tgcgctcggt gcggctttcg gcgccatggc gcagtggccg gttacgccgg
     1621 cggggcgtgc cgctcaagcg gggctcgcgg ccgatgggct ctgcttcacg gagcccacgc
     1681 cggtcctgtc gatactcgac gagctgcgca cggttgagat cggcgaccaa gaagtcgtgc
     1741 tgcggttcga caatacgggc gaccatgtcg atcgcatcgt ccatatgggc gcgcagcacc
     1801 ccgccaacgt tcaaccgtcg cggcacgggc actccattgg ccgctgggag ggcaagacgc
     1861 tcgtgatcga tacgatcggc ttcgagccaa gcggatccgg catcgcactg aacgtgcctt
     1921 cgagcgcgag caagcacacc gtcgagcggc tgacgctcac ggaggatcgc ctgcggctgc
     1981 gctacgaggt caccgtcgaa gacccgacgt atctctcggc gccggccact ctgacgcagc
     2041 agtgggacca ccgtcccgac ctcgaattct cgcaggacac cggcacctgc gatcccgagg
     2101 tcgcgaaccg gtataaggat tccgtgccga agtaacacgt tctccatcaa cgacgaggaa
     2161 tccggcgatg gccacacgac tacacggttc ccttggctcg attgcggcgc tcatcggcgc
     2221 gactgtcctc ggctcgtacc cggccgcgag ccagcaggcc ggtgccgctc cgccgctcgt
     2281 gatcacggcg ttcggcgaac agcctgcacc ggcttatacc gtcggccgca cgtcgtgggg
     2341 cgaacctgtc ttgcagggcg tctggtcgag cgacgacacg gccggcattc cgctgcaacg
     2401 gcaggagcag ttcggcgatc ggcgctatct cagcgagcag gagtacgcgg agcgcagcgg
     2461 ccaagtcgag cgcggcgtcg agcgcgccga gaacgaggcg accagcagct tccgtcaaga
     2521 cttcgcgcgc cgcgcgttct ggcagacgtc gttgatcgtc gatccgcccg acgggcgcat
     2581 gcccgcctac acggccgaag gcatgaaacg gccgatgccg cgcggcacgt acggcaacgg
     2641 accgctcgac agctacacgg acttctcgac ctacgagcgc tgcatcacgc gcggcatcgc
     2701 aggctcgtcg ctgcgcgtcg tctacggcaa cggcatgcgc atcgtgcagg cgcccggcgt
     2761 cgtggcgatc tcgtacgaga tgattcacga cacgcgcctc ctctataccg acggccggcc
     2821 gcacttgagc caagcgatcc gccaatacct cggcgattcg cgcgcgcact gggaaggcga
     2881 cgagctcgtc gtcgaaacca cgaatctcac ggaccggacg gcgatcggct tgaacggcaa
     2941 cggcaatcgt cacagctctg agatgaagct caccgagcgt ttcaagcgcg tagccgacga
     3001 cgtcatccag taccagacga ccgtggacga tcccgtcacg tacgaacgac cgttcacgat
     3061 gtcgtacccg ttgacgccgc tcgagggcgg caagctgctg ccgtacgact gccacgaagg
     3121 caactacgcg ctgctgcagt cactcggggc cgagcgcgcc gaggaccgcg cgctcgcggc
     3181 ggacaaagcg aaggggatca atcgaccgcg gcgtcccgtg caggacggcc tcggcgtcgg
     3241 cggtcagccg atctccgagg gcgcaggaat accgactcgc taaccagcga acggtttcga
     3301 cggagcgcgc gcggccgctg cgcggc
//
LOCUS       DATLEE010000543         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4332872, whole genome shotgun sequence.
ACCESSION   DATLEE010000543 DATLEE010000000
VERSION     DATLEE010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4332872"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2170)
                     /locus_tag="VM692_11705"
     CDS             complement(<1..>2170)
                     /locus_tag="VM692_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52879.1"
                     /translation="VGDEPRDIVFAGSSNRWAFITAAHRGQNAPFSPQLTTAGVGRAD
                     VWVFDANNLGTTFGGDEETIVTLFGDTPRPLAVSADGSRVYAGVFMSGNETTTLGEGM
                     VPNQTQANGGLPNPKTNYANVVAPEVGLIVKYNRNSSQWEDELGRDWSAKVKFSLPDY
                     DVFAIDATQNPPVQVAGNAGRFAHVGTTLFNMAINPVSGKLFVTNLEAFNEVRFEGPG
                     VFANSTVRGHLVESRITVIDQTTQTVDTRHLNKHIDYDSPFDPTPNAANDKSLAFPLE
                     MAFNAAGTKMYVAALGSSKVGVFTPSEIEANTFTPDEADQIEVTGGGPTGLVVSEANS
                     VLYVLTRFDNSISVVNTALNVESDHIALHNPEPVKVVEGRKFLYDARFTSARGDQACA
                     SCHIFGDFDSLAWDLGNPDDDVVNNPQDFTIGPLFLDPDFHPMKGPMTTQSLRGMDNH
                     GAMHWRGDRTGGNDEPSSQPNKGNYSEQRAFKKFNGAFTGLIGRSAQLTDGEMQKFTD
                     FILEVMYPPNPVRQLNDELTVEQAAASNIYFNRTIDTLFTCNGCHVLNRDGNPTVTRP
                     GFFGSDGRSSFENETQVFKIAHLRNLYQKVGMFGMPSEPFVNPGDNGDKGEQIRGFGF
                     LHDGSIDTVFRFFQATVFNHKATNPGMNPGFTLDPAGDAERRAMESLVLAFDTNHRPI
                     VGQQATLSTTSGADADARVNLILDRADAGDCDVVVKGVIGG"
BASE COUNT          353 a          649 c          723 g          445 t
ORIGIN      
        1 gccgccgatc acgcccttga cgacaacgtc gcagtcgccg gcatccgcgc ggtcaagaat
       61 caaattcacg cgagcatcgg catcggcgcc gctcgtggtc gacagcgttg cctgctggcc
      121 gacgatcggc cgatggttcg tgtcgaacgc gagaaccagg ctctccattg cgcgcctttc
      181 ggcgtcgccc gccggatcga gcgtaaagcc cggattcatg ccggggttgg ttgccttgtg
      241 gttgaacacg gtggcctgga agaaacggaa cacggtgtcg atgctgccgt cgtgcaggaa
      301 gccgaagccg cgaatctgct cgcctttgtc gccgttgtcg ccggggttga cgaacggctc
      361 ggacggcatg ccgaacatgc cgaccttctg gtagaggtta cgaaggtgcg cgatcttgaa
      421 cacctgcgtt tcgttctcga agctcgaacg gccgtcggag ccgaagaagc caggccgcgt
      481 cacggtggga ttgccgtcac ggttcagcac gtgacaaccg ttgcacgtga acagcgtgtc
      541 gatcgtacgg ttgaagtaga tgttcgacgc tgccgcctgt tcgacggtca gctcgtcgtt
      601 cagctggcgc accggattcg gcggatacat cacctcgagg atgaagtcgg tgaacttctg
      661 catctccccg tcggtgagct gtgcgctgcg gccgatcagg ccggtgaatg cgccgttgaa
      721 cttcttgaac gccctctgct cgctgtagtt gcccttgttg ggctgtgagc tcggctcgtc
      781 gttgccgccg gtgcgatcgc cgcgccagtg cattgcgccg tggttgtcca tgccgcgcag
      841 gctctgcgtc gtcatggggc ccttcatcgg atggaagtcc gggtcgagga agagaggacc
      901 gatggtgaaa tcctgcgggt tgttgacgac gtcgtcgtcc gggttgccga ggtcccacgc
      961 aagcgaatcg aaatcgccga agatgtggca cgacgcgcat gcctgatcgc cgcgcgccga
     1021 cgtgaagcgc gcgtcgtaga ggaacttgcg gccctcgacg actttgaccg gctccggatt
     1081 gtgcagcgcg atgtggtccg actcgacgtt gagcgcggtg ttcacgacgg agatcgaatt
     1141 gtcgaagcgg gtcagcacgt aaaggaccga gttcgcctcg ctgacgacga ggcccgtcgg
     1201 tccgccgccc gtcacttcga tctggtcggc ttcgtccggc gtgaacgtgt tggcctcgat
     1261 ctcggatggc gtgaagacgc cgactttgct cgagccgagc gcagcgacgt acatcttggt
     1321 gccggccgcg ttgaacgcca tctccagcgg gaacgcgaga ctcttgtcgt tggccgcgtt
     1381 cggcgtcgga tcgaacggcg agtcgtaatc gatgtgcttg ttcagatgac gcgtatcgac
     1441 tgtctgcgtg gtctggtcga tgacggtgat ccggctttcg acgagatggc cgcgcacggt
     1501 gctgttcgcg aacacgcccg ggccttcgaa gcgaacttcg ttgaacgctt cgaggttggt
     1561 gacgaacagc ttcccgctga ccgggttgat tgccatgttg aacagcgtcg tgccgacgtg
     1621 cgcgaagcgg ccggcgttgc ctgcaacctg aaccggagga ttctgcgtcg catcgatcgc
     1681 gaacacgtcg tagtcgggca ggctgaactt gaccttggcc gaccagtcgc ggccgagctc
     1741 gtcttcccac tgcgaagagt tgcggttgta cttgacgatt aggccgactt cgggcgctac
     1801 gacgttcgca tagttcgtct tcgggttcgg caggccgccg ttggcctgcg tctggttcgg
     1861 aaccattcct tcgccgagtg tcgtcgtctc gttgcccgac atgaacacgc cggcgtacac
     1921 gcgactgccg tcggcgctga ctgcgagcgg gcgcggcgtg tcgccgaaca gcgttacgat
     1981 ggtttcctcg tcgccgccga aggtcgtgcc gagattgttc gcgtcgaaca cccacacgtc
     2041 ggcgcgtccg acaccggccg tcgtgagttg cggactaaac ggcgcgtttt gaccgcgatg
     2101 cgcggcggtg atgaatgccc agcgattgct cgagccagcg aacacgatgt cgcgaggctc
     2161 atcgccgacg
//
LOCUS       DATLEE010000544         3120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_637598, whole genome shotgun sequence.
ACCESSION   DATLEE010000544 DATLEE010000000
VERSION     DATLEE010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637598"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2328)
                     /locus_tag="VM692_11710"
     CDS             complement(<1..2328)
                     /locus_tag="VM692_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin domain-containing protein"
                     /protein_id="HUQ52880.1"
                     /translation="MRAELVSLSGNGSNLSFSVIGEGDVVIDLKNPSGLSLNVTGATV
                     KSLVGEILTLNIGAIGPHDVSVTLGSVATNRAPTITSNGGGDTATIGVAENTTAVATV
                     TATDPDAGQTRSFSIVGGADQALFTISAGGVLSFVAPRNFESPADSDRNNSYVVQVRV
                     ADNGSPILSDTQTITVNVADVAEATPNQAPTYTVSDGKVTTMIGSPWNFANALAIQPD
                     GNILVAGDATIGSSREFALARFNSNGSLDTTFDGDGKVTTSFGAGESFGNSVALRDGK
                     IIVAGYSFSTAGTAEDFAVARYNANGTLDTTFDGDGRVTTDFGGNTDVGRSVAIQADG
                     KILVAGESRIGSNYDFSVARYNANGTLDTTFDGDGRVRTDFGSDGDIAWTLAVQGDGK
                     IVVGGWGGGGFTLARYNANGSLDTTFDGDGRVATIVGGPNTGFLRSIVIQPDGKILAA
                     GEAFNGRADTDIVVLRYNANGTLDTSFGTGGIVFTNTPTVSDAANDLLLQPDGRIIVA
                     GHVGAAAGSDFFLVRINADGTIDGAVTTDFGNVSEAAKAIERQADGKLVVAGFSYDSF
                     AVARYNADGSLDPTFDPTKVNTLNGTPAYTAGGAPVVLDTDVRAYDPDLAASGNYSGA
                     TLTLARSAGANAQDVFSATGDLATLTQGAALVYDGVTVGTVTTNSAGRLLLTFNSSAT
                     QGYVDDVLQSIAYSNSSATPPASVQINWTFSDSNAGAQGTGGALMATGSTTVTISTPA
                     PNQPPVITSNGGSDATSIAVTENSTAVTTVTATDPN"
     gene            2516..2710
                     /locus_tag="VM692_11715"
     CDS             2516..2710
                     /locus_tag="VM692_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52881.1"
                     /translation="MAVTVLPETLVVIAELWNHSIRCARSASVTNSAPALYAREMKSV
                     NICVVYGEGTLELSLHSVAE"
     gene            2746..>3120
                     /locus_tag="VM692_11720"
     CDS             2746..>3120
                     /locus_tag="VM692_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52882.1"
                     /translation="MSRELPPLARPFRRRLRGGHVLELDERKIELHVWREIHLVLRGE
                     RRRQEADGARKARADPGEAAGQVVVVLEDLLRCGEVLVRAGHRGALDGERPSEDLRHL
                     LLDDLLRALELELDLCRCQLIHV"
BASE COUNT          497 a          982 c         1020 g          621 t
ORIGIN      
        1 gtttgggtct gtcgcagtga cggtagtcac ggccgtggaa ttttccgtca ccgcgatgct
       61 tgtcgcgtcg ctgccgccgt ttgaagtaat cacagggggc tggttcgggg ccggggtcga
      121 gatcgtcacg gtggtgctgc cggtcgccat gagcgctccg ccggtgccct gcgctccggc
      181 gttgctatct gagaacgtcc agttgatctg caccgacgcc ggcggcgttg cgctgctgtt
      241 cgagtatgca atcgactgca gcacgtcgtc gacatagccc tgcgtcgcac tgctgttgaa
      301 ggtgagcagg aggcgaccgg ccgagttcgt cgtgaccgtg ccgaccgtaa cgccgtcgta
      361 cacgagcgca gcgccttgcg tcagcgtcgc gaggtcaccg gtcgcggaga acacatcctg
      421 cgcattcgcg ccggcgctgc gcgcgagcgt gagcgtcgcg ccgctgtaat tgccgctcgc
      481 ggcgaggtcg ggatcgtacg cgcgcacgtc ggtgtccagc acgaccggtg cgccgccggc
      541 cgtatatgcg ggcgtgccgt tcagcgtgtt tactttcgtg ggatcgaagg tcgggtcgag
      601 actgccgtcg gcgttgtaac gcgcaaccgc gaagctgtcg tagctgaaac cggcaacgac
      661 gagcttgccg tcggcctgcc gttctatcgc ttttgcggct tccgacacgt tgccgaagtc
      721 ggttgtgacc gcgccgtcta tcgttccatc tgcgttgata cgaacaagga agaaatcgct
      781 gcctgccgcg gcgcccacgt ggccggcgac gatgatcctg ccgtcgggct gcagcagcag
      841 gtcgttcgcc gcgtcactta cggtcggcgt gttcgtaaac acgatgccgc cggtgccaaa
      901 actcgtgtcc agcgtgccgt tcgcgttgta acgcagcacg acgatgtcgg tgtcagcacg
      961 gccgttgaaa gcctctcccg cggcgaggat cttgccatcg ggctgaatga ctatgctgcg
     1021 caggaaaccc gtgttcggcc cgccgacgat cgtggcgact ctgccgtcgc cgtcgaacgt
     1081 cgtgtcgagc gaaccattcg cgttgtagcg ggcaagcgtg aagccgccgc caccccaccc
     1141 gccgacgacg atctttccgt cgccctgcac ggcgagcgtc cacgcgatgt cgccgtcgct
     1201 gccgaagtcg gtcctgaccc ggccgtcacc gtcaaacgtc gtgtcgagtg ttccgttggc
     1261 gttatagcgc gcgacgctga agtcatagtt ggagccaatg cgcgattcgc cggctacgag
     1321 gatcttgccg tcggcctgaa tcgcaacgct gcggccgacg tcagtgttgc cgccgaagtc
     1381 ggtcgtaact ctgccgtcgc cgtcgaacgt ggtgtcgagc gtgccgttgg cgttgtatcg
     1441 cgcgaccgca aagtcttcgg cggtgccggc ggtgctgaag ctatagcccg cgacgatgat
     1501 cttgccgtcc cgcagcgcga cgctgtttcc aaaggactcg cccgcaccaa aagaggtcgt
     1561 gaccttgcca tcgccgtcga acgtcgtgtc gaggctgcca ttgctgttga agcgcgccag
     1621 cgcgaactcg cggctgctgc cgatggtcgc gtcgccggcg acgagaatat tgccgtccgg
     1681 ctggatcgcg agggcgttcg cgaaattcca cgggctgcca atcatcgtcg ttactttgcc
     1741 gtcgctgacc gtgtacgtcg gcgcctggtt aggcgtggct tcagcaacgt cggcgacgtt
     1801 cacggtaatc gtctgcgtgt ccgaaaggat ggggctgccg ttgtccgcaa cccgcacctg
     1861 gacgacgtag ctgttgtttc ggtcgctatc ggccggcgat tcgaagttcc gcggcgcgac
     1921 gaagctgaga acgccaccgg cgctgatcgt gaacagcgcc tggtcggcgc cacccacgat
     1981 cgaaaacgag cgtgtctgcc ccgcgtccgg atcagtcgcc gtgacagtcg ctactgcagt
     2041 cgtgttttcc gccacgccga tcgttgctgt atctcccccc ccgttcgatg tgatggtggg
     2101 cgcccggttg gtggcgaccg agcccagcgt taccgagacg tcatgcgggc cgatcgcgcc
     2161 gatgttcagc gtcaatattt cgcccacgag gctcttcaca gtggcgccgg tgacgttcag
     2221 cgacaagccc gaagggttct tcagatcgat cacgacgtcg ccttcgccaa tgaccgagaa
     2281 gctcaggttg ctgccattcc cgctcaacga gacgagctcg gcgcgcatcg ggagtcgcgt
     2341 gatgtgcgtt acgtcgtcgg ccgttacgcc gaggttgatc gtgtaggagc cgccgttgcg
     2401 cggcaaaaac acgctgtcgg tatcgtaggc gtaccacccc gtgacattct tgatgacctg
     2461 gcttcctacg ttatccaggt caagcgcgaa cttacccgca tcgggcgagg cgacagtggc
     2521 ggtgaccgtg ctgccggaga cgctcgtcgt tatcgccgag ttatggaacc actcgattcg
     2581 ctgcgcgagg tcggcgagcg tcacgaactc cgcgccggcg ttgtacgcgc gagagatgaa
     2641 atccgtgaac atctgcgtcg tatacggcga agggacgctc gagttgtccc tccactcggt
     2701 ggccgagtag tcgtgccacg gccagacgat cacgggcacg tcggtgtgag ccgtgagctc
     2761 cctccactcg cgcgcccatt ccgccgacgc ctgcgcggcg gtcatgttct ggaactcgac
     2821 gagcgaaaaa tcgaacttca cgtttggcgc gagatacacc ttgttctgcg cggcgagcgc
     2881 cggcgtcagg aagccgatgg cgcccggaaa gcccgcgccg accccggaga agccgccgga
     2941 caggtagtcg tagtactgga ggatctgctg cgctgtggcg aggttctcgt tcgtgccggg
     3001 caccgcggcg ccctcgacgg tgaacggccg tccgaggatc tgcgacatct gctgctcgat
     3061 gatctgcttc gagctctgga actcgaactg gatctgtgcc ggtgtcagct gattcacgtt
//
LOCUS       DATLEE010000545         4578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6276212, whole genome shotgun sequence.
ACCESSION   DATLEE010000545 DATLEE010000000
VERSION     DATLEE010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6276212"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VM692_11725"
     CDS             complement(<1..248)
                     /locus_tag="VM692_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52883.1"
                     /translation="MRELNSLVTKVTPAESRVTEVLALAQPIAFGRVYRLRTVANAIG
                     AQLKQCDASEPLPLLEKVGAGTQLTEAERTQDVVAAQGV"
     gene            307..888
                     /locus_tag="VM692_11730"
     CDS             307..888
                     /locus_tag="VM692_11730"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HUQ52884.1"
                     /translation="MTNPVRQRRLQVSAALAVAVVCWLAAAGNALAQDAAQAAAAHSV
                     KAAYLFKLPAYVDWPAQRFDSPDAALTIGVLGADEVADALAALTSGRTVNGHPVAVRR
                     LQSDEGIDGVHVLFFGSTAAERAGRIAADAAALNILTVTDLQAAFDDSVIDFVTVNGR
                     VRFEVRLMAAERNGLRLHAGLLDVAARVHGARR"
     gene            885..2363
                     /locus_tag="VM692_11735"
     CDS             885..2363
                     /locus_tag="VM692_11735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ52885.1"
                     /translation="MTIEMRKGSVRSKLLLAVLATTGMALLMTGAAVAYFDLRTFRQS
                     SIDDLTAIGDLLGLAATPALEFDDPQTAQEYLSLLRAKPAVTAAAIYAPNGALFARYA
                     AGEGGDFPELPGQDGYEIGGNELTLFKRVIADHEILGTVYVRAHYDVTSRIARYLGIL
                     AAVLLPSLAAAALMAHRLHRGITTPIRGVIDVARNVLEKRDYSARAPKGHDDELGMLV
                     DAFNNMLDEVGQRTAVLEHAEEKLRTLNVELEQRVGARTAELEAAKKELEGFSYSVSH
                     DLRAPLRAIGGFAELLREDHAEQLDAEARRKLEVIRGEASRMGSLIDDLLAFSRLGRK
                     SLTPGDLDMTELVNSVFGRLRAEPGGERVELKLGRLPRACGDRALLEQVWVNLLSNAV
                     KFSSKKDAPVVDVGAITQEREHVFYVRDNGAGFDPRYAAKLFGVFQRLHDAAEFPGTG
                     VGLALVQRIVVRHGGRVWADSKPGEGATFHFTLPKESGHAGV"
     gene            2350..2793
                     /locus_tag="VM692_11740"
     CDS             2350..2793
                     /locus_tag="VM692_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUQ52886.1"
                     /translation="MQEFEHVEILLAEDNPHDAELAIRGLEKQKLANRITWVKNGEEA
                     LDYVFRRGAFSGRTDAGPRLILLDLKMPKVDGIEVTRAVKADERTRHIPIVIMTSSQE
                     EKDVANSYSCGANSYIVKPLDFKSLVDVASQVGFYWLAINRGQPT"
     gene            2861..3661
                     /locus_tag="VM692_11745"
     CDS             2861..3661
                     /locus_tag="VM692_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HUQ52887.1"
                     /translation="MTEPISSAQYFPWDGGAVFVGTAGEFPAHAHQAIQICLLFEGRV
                     RLRTTNDEPWADYDLAIVPSRHSHAMDGAKVHYGATLFVEPETREGRILTERFLQSGI
                     AAVDRRPADAALLELRAAALQRRGQRTIVDRARAVVQSLTEHTEPSISSDERILRAVK
                     YINEHLSAPITLAQVARVAYLSPSRFRHLFAEQTGMRLREYVLWRRFVNVWELRMRGV
                     SLSDAAHAAGFADSAHLARTSRRMFGIPPSLMDLSAAAEPGTLAATSH"
     gene            complement(3940..>4578)
                     /locus_tag="VM692_11750"
     CDS             complement(3940..>4578)
                     /locus_tag="VM692_11750"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HUQ52888.1"
                     /translation="GEAANSRFEALAASEGFLVATLQAGTEGNRWNVPITAERPDDVR
                     YVSDVIDHVAARACTDAARVYATGFSGGARMSSLLACKLNDRIAAIAPMSGLRWPGPC
                     DGRAIPVLTFHGLADPQNTYDGHASNRGGEWEESVPDALAGWAKHNGCDGAAKLDDPP
                     GPLSTLAYSGCDAEVRLVRVDGLVHRWAHDEVDATAEAWKFFRAQRLPSVTH"
BASE COUNT          746 a         1549 c         1562 g          721 t
ORIGIN      
        1 acgccctgcg ccgccacgac atcctgcgtg cgctcggcct ccgtgagctg cgtgcccgcg
       61 ccgaccttct cgagcagcgg cagcggctcc gacgcgtcgc actgcttgag ctgggcgcca
      121 attgcgttgg cgacggtacg aagacggtag acgcggccga acgcgatcgg ctgcgcaagc
      181 gcgagcacct ccgtgacacg cgactcggca ggcgtgacct tcgtcacgag actatttagc
      241 tcgcgcacat aaactcgatc cgctgtgccg caccgagcgc ggcgcctttg cttggcacta
      301 gggccgatga cgaatcccgt ccgacaacgc cgcttgcagg tctccgctgc cctcgccgtc
      361 gccgtcgtgt gctggctcgc ggcggcgggg aacgcgctcg cgcaggacgc ggcacaggcg
      421 gccgcagcgc acagcgtcaa agccgcctat ctcttcaagt tgccggccta tgtggattgg
      481 ccggcccaac gcttcgatag cccggacgcg gcactgacga tcggcgtgtt gggcgccgac
      541 gaggtcgccg acgcgctcgc cgcgctgacg tcgggccgca ccgtgaacgg ccacccagta
      601 gccgtgcgac ggctgcagtc ggacgagggc atcgacggcg tgcacgtgct gtttttcggc
      661 agcaccgccg ctgagcgagc cggccgcatc gccgcggacg cggccgcgct gaacattctt
      721 acggtcacgg atttgcaagc ggcgtttgac gacagcgtca tcgacttcgt gaccgtcaac
      781 ggcagagtcc gcttcgaggt ccggctgatg gccgccgagc gcaacggcct ccggctgcac
      841 gcgggcttgc tcgacgtcgc ggcgcgcgtg catggagcgc ggcggtgacg atagagatgc
      901 gcaagggctc cgtgcgcagc aagctgctgc tcgccgtgct cgcgacgacg ggcatggcgt
      961 tgctgatgac gggcgccgcc gtcgcgtatt tcgatctgcg gaccttccgt cagagctcga
     1021 tcgacgatct gacggcgatc ggcgatctgc tcggcctcgc tgcgacgccg gcgctcgagt
     1081 tcgacgatcc gcagacggct caggaatatc tctccctgtt gcgcgccaag ccggccgtca
     1141 cggctgccgc gatctatgcg ccgaacggcg cactgttcgc gcgctacgcg gccggcgagg
     1201 gaggagactt tcccgagctg ccgggccagg acggttacga gatcggcggc aacgagctga
     1261 cgctgttcaa gcgcgtcatc gccgaccatg aaattctggg cacggtctac gttcgtgcgc
     1321 attacgatgt gacgagccgc atcgctcgct acttgggcat cctcgcggcg gtgctgctgc
     1381 cgagccttgc ggccgcggcg ttgatggcgc atcggctgca ccgcggcatc acgactccaa
     1441 tccgcggcgt catcgacgtc gcgcgcaacg tgctcgagaa acgcgactac tcggcgcgcg
     1501 cgcccaaggg ccacgacgac gagctcggca tgctcgtcga tgcattcaac aacatgctcg
     1561 acgaggtggg gcaacgcacg gccgtcctcg agcacgccga ggagaagctg cgcacgctga
     1621 acgtcgagct cgagcaacgc gtcggcgcgc gcacggcgga gctcgaggcc gcgaaaaagg
     1681 agctcgaggg cttcagctat tcggtgtcgc acgacctgcg cgcgccgctg cgcgcaatcg
     1741 gcggcttcgc cgagctgctg cgcgaagacc acgccgaaca gctcgacgcc gaagcgcgac
     1801 gcaagctcga ggtcatccgc ggcgaggcgt cgcgcatggg ttcgttgatc gacgacctac
     1861 tggcattttc gcggctcggc cgcaagtcgc tgacccccgg cgacctcgac atgaccgagc
     1921 tcgtaaacag cgtattcggc cggctgcgcg ccgagcccgg cggcgagcgc gtcgagctga
     1981 agctcgggcg tttgccgcgc gcctgcggcg accgcgccct gctcgagcag gtgtgggtca
     2041 acctgctgtc gaatgccgtg aagttctcgt cgaagaagga cgctcccgtc gtcgacgtcg
     2101 gcgcgatcac gcaagagcgc gagcacgtgt tctacgtccg cgacaacggt gcgggcttcg
     2161 atcctcggta tgcggcgaag ctgttcggag tgtttcaacg attgcacgac gccgcggagt
     2221 tcccgggcac aggcgtcgga ctcgcgctcg tgcaacgcat cgtcgtgcgc cacggcggcc
     2281 gcgtctgggc cgacagtaag cccggcgagg gcgcgacgtt ccatttcacg ctgccgaagg
     2341 agagcggcca tgcaggagtt tgaacatgtc gagattttgc tcgcggaaga caacccgcac
     2401 gatgccgagc tggcgattcg ggggctcgag aagcaaaagc tcgctaacag gatcacatgg
     2461 gtcaagaacg gcgaggaagc gctcgactat gtatttcgtc gcggggcgtt ctcggggcga
     2521 accgacgccg ggccgcgctt gatcctgctc gacctcaaga tgccgaaggt cgacggtatc
     2581 gaggtcacgc gggccgtcaa agccgacgag cgcacgcgcc acatcccgat cgtcatcatg
     2641 acctcgtcgc aagaagagaa ggacgtcgcg aacagctaca gctgcggcgc gaacagctac
     2701 atcgtgaagc cgctcgactt caagtcgctc gtcgacgtgg cctcgcaagt aggcttctac
     2761 tggctcgcga tcaaccgcgg tcaaccgacc tgatcgcgaa gccggcggcg cgggctcgaa
     2821 cgaaccggcg gtcgtaggat gccggtagac tgagggcccg atgaccgagc cgatcagcag
     2881 cgcgcagtac tttccgtggg acggcggcgc cgtcttcgtc ggcacggccg gcgagttccc
     2941 cgcccacgcg catcaagcaa tccaaatctg cctgctattc gaagggcgcg ttcggctgcg
     3001 cacgacgaat gacgagccgt gggccgacta cgacctcgcg atcgtgccgt cgcggcactc
     3061 gcacgccatg gacggcgcga aggttcacta cggcgcgacg ctgttcgtcg agcccgaaac
     3121 ccgtgaaggg cgaattctca cggaacgctt cctgcagagc ggcatcgccg cggtcgatcg
     3181 gcggcccgcc gacgccgctc tgctcgagct gcgtgccgct gcattgcaac ggcgcgggca
     3241 gcgcacgatc gtcgaccgtg cacgcgccgt cgtgcagtcc ttgacggagc acacggaacc
     3301 gtcgatcagc agcgacgaac gcatcctgcg cgcggtgaag tacatcaacg agcacctgtc
     3361 cgcgccgatc acgctcgcgc aggtcgcgcg cgtcgcctat ctgtcgccga gccgcttccg
     3421 ccatctgttc gccgagcaga cgggcatgcg gttgcgcgag tacgtgctct ggcggcgctt
     3481 cgtcaacgtt tgggagctca ggatgcgcgg cgtttcattg tcggacgccg cgcatgcggc
     3541 ggggtttgcc gactccgcgc acctcgcacg cacgagccgc cgcatgttcg ggattccgcc
     3601 gtcgttgatg gacctgtcgg cggcagccga gcctggcact ctcgcggcaa catcacatta
     3661 gccgtttcgt tcaaggcgcc tgagcgggta gctcgtacag tgcatccgca cttcgccaca
     3721 gcaaccccat aggagaaacg tcatgaacga agcgaaccag aatgtttgtg cctgcaaccc
     3781 gtgcgtcggc tcgagctgca cctgcggctg ccagagcacc gtcgcgcaag cgacatgcga
     3841 gtgcggcgtc gactgccgct gcgggggtgc ctgcggccag gcgtgagcgg cgagcgaggc
     3901 gcggcagcac gcgctgccgc gctggcccgt tggcggttgt cagtgcgtga cgctcggcag
     3961 gcgctgcgcg cggaagaact tccacgcctc ggccgtcgca tcgacctcgt cgtgcgccca
     4021 gcggtgcacg agcccgtcga cgcgcacgag ccgaacctcg gcgtcgcagc cggagtaagc
     4081 cagcgtcgac agcggccccg gcgggtcgtc gagcttggcc gcgccgtcgc agccgttgtg
     4141 cttggcccag cccgcgagcg cgtccggcac gctttcttcc cactcgccgc cgcggttcga
     4201 ggcgtggccg tcgtaggtgt tctgcggatc ggcgagccca tgaaacgtca gcaccggaat
     4261 cgcccggccg tcgcacggcc ccggccaacg caggccggac atcggcgcga tcgcggcgat
     4321 gcgatcgttg agcttgcagg ccagcagcga cgacatgcgt gcgccgcccg agaagcccgt
     4381 cgcatagacg cgcgccgcgt ccgtgcaggc gcgcgcagcg acgtggtcga tgacgtcgga
     4441 gacgtagcgc acgtcgtcgg ggcgctcggc ggtgatcggc acgttccagc ggttgccttc
     4501 cgtgccggcc tgcagagtgg caacgaggaa gccttcgctg gccgcgagcg cctcgaagcg
     4561 cgagttggcg gcttcgcc
//
LOCUS       DATLEE010000546         3906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2125387, whole genome shotgun sequence.
ACCESSION   DATLEE010000546 DATLEE010000000
VERSION     DATLEE010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125387"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VM692_11755"
     CDS             <1..761
                     /locus_tag="VM692_11755"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUQ52889.1"
                     /translation="ALFDGEQADVATNAETQALRQSVDHPELRTILTVSEGFYRLVAR
                     KSAGISTVADLRGKRITTAPATSSAYYLHKLLRTAGLTTADVTIVTVNPLSNMPAALL
                     NREVDAVTIWEPEVENAAAAIGADAIEFQPRPIIYRELFGLHTTVAKLADPAKRREIV
                     AFVRAVVRASERIRANPAEAWPLVAQRTGFERALIEKVWQHEGYPGAIVPDLLDVMVE
                     EDPFLAPERNRMPRTRAQLAVMIDSSVLREALAE"
     gene            770..1795
                     /locus_tag="VM692_11760"
     CDS             770..1795
                     /locus_tag="VM692_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUQ52890.1"
                     /translation="MHCRARRPALFALAALVALPVLATAQSSPLTIEKQGAFAIGGTV
                     LGDADSSSLRCDHGVVEFQIPPNARSVNLLLWHSASAVAWQQRWDGGEGFQTIFVRRG
                     FPVYLWDGPRVGRANWGCATTTYEPGVGRDQQNFVAWRFGAAFPDWFDGVQFPSNDAE
                     AWNQAMRARYQEFDTIENAQLETDAAAVLADKIGPTVALTNSAGGLRALLTAMKSDNI
                     VGIVAYENVGYVYPTGEAPDVEPGPFGPVEVPLEQFRKLTRIPMQMVWGDNTDKSDRY
                     RPTVEESRRWVELVNKHGGKAEILLLPDAGLHGNTHIPFADLNNVAVADLLSKYLAAH
                     GLDKRGR"
     gene            complement(1796..2233)
                     /locus_tag="VM692_11765"
     CDS             complement(1796..2233)
                     /locus_tag="VM692_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HUQ52891.1"
                     /translation="MDKINPLFTATATASGGRNGRSESDDGIVKVDLSVPKAMGGPGR
                     PGTATPEHLFATGYAACFGGALDFVGKQHKKDASKAKVTCAVSIGPRAGGGFGLAVKL
                     RVEDKSLPQAELTTLAREAHEKICPYSHATRGNVNVQLDVVGA"
     gene            complement(2292..2759)
                     /locus_tag="VM692_11770"
     CDS             complement(2292..2759)
                     /locus_tag="VM692_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUQ52892.1"
                     /translation="MRRPQLRGAAALLAAIVVVAIDAAAAQTARKPAATSWDGVYTTE
                     QAQRGQVAYTGPCDRCHGYKLDGASEDPDMLPAPPVAGPKFLRKWNGRSLAVLLEYLR
                     TTMPSNNPGYLSDGEVADVVAYMLATSGVPAGSEPLRPELDVLAAVVIASPPQ"
     gene            complement(2761..3378)
                     /locus_tag="VM692_11775"
     CDS             complement(2761..3378)
                     /locus_tag="VM692_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52893.1"
                     /translation="MEAHATHIVVWDAPAAVERAAPLRFKVGVKCAAECSSAGRRVAI
                     RDAQGRELASAVVGDVAWPGTAALNYAEFELPAPDADGLHAWVAALEADAAAAPDHAP
                     HAAATSQFQVRVVPAPECTVKVVAVDAASRTPVGGARVVVHPYRAVTDSQGIAELRVP
                     KGAYRLFVSGRDYFPFRSDGDVRADVTIEAELYADRGPSDAELWS"
     gene            complement(3384..>3906)
                     /locus_tag="VM692_11780"
     CDS             complement(3384..>3906)
                     /locus_tag="VM692_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HUQ52894.1"
                     /translation="NEGSQAPHYHSGPIWAIFDQDSVERAEWELRFPYVGDNGYYFKA
                     DTIEELGAKIHKGHEFQRVPLKHLAATVAQWNGYVDAGKDPDFEREGDAPMHKIVKPP
                     FHALAIMVVWHDSYGGLRVNGKQQVVDMRGQVIPGLYAGGEAVGGFQKHGLGKGHVHG
                     FIAGTNAAAEKPA"
BASE COUNT          604 a         1353 c         1323 g          626 t
ORIGIN      
        1 ccgctctatt cgacggcgag caggccgacg tcgcgacgaa cgccgagacg caggcgctac
       61 gccaatccgt cgaccatccg gagctgcgca ccatcctgac ggtgtccgag ggcttctacc
      121 gtctcgtcgc ccgcaagtcg gcgggcatct cgacggtcgc ggatctgcgc ggcaagcgca
      181 tcacgacggc gccggccacc tcgtcggcct attacctgca caagttgctc cgcacggccg
      241 gcctgacgac ggccgacgtg acgatcgtga cggtgaaccc gctgtcaaac atgccggcgg
      301 cgttgctgaa ccgcgaggtc gacgccgtga cgatctggga gcccgaggtc gagaacgcgg
      361 cggcggcgat cggcgccgac gccatcgagt tccagccgcg gccgatcatc taccgcgagc
      421 tattcggctt gcacactacg gtcgcgaagc ttgcggatcc ggccaagcgc cgcgagatcg
      481 tcgcgttcgt gcgcgccgtc gtccgcgcca gcgagcgcat tcgcgcgaac ccggccgaag
      541 cctggccgct cgtcgcgcaa cgcaccggct tcgagcgcgc gctgatcgag aaagtttggc
      601 agcacgaggg ctacccgggc gcgatcgtgc ccgacctgct cgacgtcatg gtcgaggagg
      661 acccgttcct cgcgccggaa cgcaaccgca tgccgcgcac acgcgcgcag ctcgccgtga
      721 tgatcgattc gagcgtgctg cgcgaagcgc tcgccgagtg agcgcaccta tgcattgccg
      781 tgcacgccgg cccgccctgt tcgccctcgc cgcgctcgtc gcgctccccg tgctggcgac
      841 ggcgcaaagc tcgccgctga cgatcgagaa acagggcgcg ttcgcgatcg gcggcacggt
      901 cctcggcgac gccgactcgt cgtcgctgcg ctgcgatcat ggcgtcgtcg agtttcaaat
      961 cccgccgaac gcgcgcagcg tcaatctact gctgtggcac agcgcgagcg ccgtcgcgtg
     1021 gcagcagcgc tgggacggcg gcgaaggctt ccagacgatc ttcgtgcggc gcggcttccc
     1081 cgtgtatctg tgggatgggc cgcgcgtcgg ccgtgcgaat tggggctgcg cgaccacgac
     1141 ctatgagccc ggtgtcggcc gcgatcagca gaacttcgtg gcgtggcgct tcggcgctgc
     1201 gttccccgat tggttcgatg gcgtgcaatt tccgagcaac gatgccgagg cgtggaacca
     1261 ggccatgcgc gcgcgctatc aggagttcga cacgatcgag aacgcgcagc tcgagacgga
     1321 cgccgcggcc gtgctcgcgg acaagatcgg cccgacggtc gcgctgacga attcggcggg
     1381 cggcttgcgc gccctgctga ccgcgatgaa gagcgacaac atcgtcggca tcgtcgccta
     1441 cgagaacgtg ggctacgtct atccgaccgg tgaggcgccc gacgtcgagc cgggcccgtt
     1501 cggccccgtc gaagtgccgc tcgagcagtt tcgcaagctg acgcgcatcc cgatgcagat
     1561 ggtctggggc gacaacaccg acaagtcgga ccgctatcga ccgacggtcg aggagtcgcg
     1621 gcgctgggtc gagctcgtca acaagcacgg cggcaaagcc gaaatcttgc tgctgccgga
     1681 cgcggggctg cacggcaaca cgcacattcc gtttgcagac ctcaacaacg tcgcagttgc
     1741 cgacctactg tcgaagtatc tcgccgcgca cggcttggac aaacgcgggc gctagttacg
     1801 cgcccacgac gtcgagctgc acgttgacgt tgccgcgtgt cgcgtgcgag tacgggcaaa
     1861 tcttctcgtg cgcttcgcgc gcgagcgtcg tgagctcggc ttgcggcagc gacttgtcct
     1921 cgacgcgcag cttcacggcc aacccgaagc cgccgccggc gcgcgggccg atcgaaacgg
     1981 cgcacgtgac cttggccttc gacgcgtcct tcttgtgctg cttgccgacg aaatcgagcg
     2041 cgccgccgaa acaggcggcg tagccggtcg caaacaagtg ctccggcgtc gccgtgcccg
     2101 gcctgccggg gccgcccatc gctttcggga cggacagatc gactttgacg atgccgtcgt
     2161 cggattcgga gcggccgtta cgtccgccgc tcgcggtcgc cgtcgccgtg aagagcggat
     2221 tgatcttgtc catggtgggc gtcctcgttg cgtggcggaa gccacagact aaccgattcc
     2281 gccggcgcgc ttcactgcgg cggcgacgcg atgacgactg cggcgagcac atcgagctcg
     2341 ggccgcagcg gctcgctgcc ggccggcacg ccgctcgtcg ccagcatgta cgcgacgacg
     2401 tcggcgactt cgccgtcgct caggtagccc gggttgttcg acggcatcgt cgtgcgcaaa
     2461 tactcgagca gcacggccag cgagcggccg ttccacttgc ggaggaactt ggggccggcg
     2521 accggcggcg ccggcaacat gtccgggtct tcggacgcgc cgtcgagctt gtagccgtgg
     2581 cagcggtcgc aaggtcccgt atacgcgact tggccgcgtt gcgcctgctc cgtggtgtag
     2641 acgccgtccc agctcgtcgc ggccggcttc ctcgcggtct gtgcagcggc tgcatcgatc
     2701 gcgacgacga cgattgcggc caacagcgcg gcggctccgc gcagctgcgg gcgcctcatc
     2761 ctacgaccag agctccgcgt cgctcggccc gcgatcggcg tagagctcgg cctcgatcgt
     2821 aacgtcggcg cgcacgtcgc cgtcgctgcg aaacggaaaa tagtcgcggc ccgagacgaa
     2881 cagccgatac gcgcctttcg gcacacgcag ctccgcaatg ccctgcgaat cggtgacagc
     2941 ccgataggga tgcacgacga ctcgggcgcc gcccacgggc gtgcggctcg ccgcatcgac
     3001 ggcaacgact ttgacggtgc actcgggagc cggcacgacg cgcacctgga actgcgaggt
     3061 cgcggcagcg tgtggcgcat gatcgggcgc agcggcagcg tcggcttcga gagcggccac
     3121 ccaggcatgc aagccgtcgg cgtcgggcgc gggcaactcg aactcggcgt agttcagcgc
     3181 ggcggtgcct ggccacgcga cgtcgccgac gaccgcggat gccagctcac gcccttgcgc
     3241 atcgcggatc gcgacgcgcc ggcccgcgct gctgcattcg gccgcgcact tgacgccgac
     3301 cttgaaacgc aacggcgcag cgcgctcgac cgccgccggc gcgtcccaga cgacgatgtg
     3361 cgtcgcgtgc gcctccatcg gcgctacgcg ggtttttcgg ccgcggcgtt cgtgcccgcg
     3421 atgaagccgt gcacgtggcc tttgccgaga ccgtgcttct ggaagccccc gacggcctca
     3481 ccgcccgcgt acaggccagg gatgacttgg ccgcgcatat cgacgacttg ctgcttgccg
     3541 ttgacgcgca gcccgccgta cgagtcgtgc cagacgacca tgatcgccag cgcgtggaac
     3601 ggcggcttca cgattttgtg catcggcgcg tcgccttcgc gctcgaagtc gggatccttg
     3661 cccgcgtcga cgtagccgtt ccattgcgca acggtcgcgg cgagatgctt cagcgggaca
     3721 cgctggaact cgtggccctt gtgaatcttc gcgccgagct cttcgatcgt gtcggccttg
     3781 aagtagtagc cgttgtcgcc gacgtacggg aaccgcagct cccattcggc gcgctcgacg
     3841 gagtcttgat cgaagatcgc ccagatcggt cccgagtgat aatgcggcgc ctgcgaccct
     3901 tcgttg
//
LOCUS       DATLEE010000547         3932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2873372, whole genome shotgun sequence.
ACCESSION   DATLEE010000547 DATLEE010000000
VERSION     DATLEE010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2873372"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VM692_11785"
     CDS             <1..791
                     /locus_tag="VM692_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52895.1"
                     /translation="TRMGIEKSMLETGTTIKVRGNVGRRNAHALWVSNILLPNGTELL
                     AAPGATPHWGSKTVGDATEFFVAGDLKLPAGSERSFFRVWSPLISAFPRPRGMPALTE
                     AGQRAQARYETGEQAVGDCEVPGMPYAMMSPYPIEIVQQGNRLLIRGEAYDLVRTVDL
                     AAPATTPAPTPLGSSVAKLSGDELVIETSRIDYHSYGDLGPAQSNQSHVVERFKLSAD
                     GTKLNYEITVTDPVMLREPWLWGGSFVYREGAEIKPWRCGADRA"
     gene            complement(810..1244)
                     /locus_tag="VM692_11790"
     CDS             complement(810..1244)
                     /locus_tag="VM692_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52896.1"
                     /translation="MNPVIQHNVLPEIRLWRVANAIHANISRSVVRQPVILEHRPEEN
                     TSRPTRRERLDLALNILNLFVTPQRGALDEREPVKCFRGYCSAFAARHHEQFMSEFVD
                     DLAETGGTIESARIRAWIDTRHASSAAVFADKIDPERLLESA"
     gene            complement(1329..1796)
                     /locus_tag="VM692_11795"
     CDS             complement(1329..1796)
                     /locus_tag="VM692_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52897.1"
                     /translation="MIVEGLETYARVSGPWLQAWNAANAMNVSATRIAIGQVELAALT
                     TRFMNDRLAAYASFDGHVEPLVRRLDRLTEQFADNYATQVRTIYSSWADVLRDDRPLT
                     DAVGRAAERTDERFEGARPQDAAKREEATKQEGAKRESRREERPPQGRDTAAH"
     gene            2177..>3932
                     /locus_tag="VM692_11800"
     CDS             2177..>3932
                     /locus_tag="VM692_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52898.1"
                     /translation="MPGELVVEPATLINLGFEWWIEGDDNRNASVAVSFRKRGESAWQ
                     PALPMLRLHGERVYSESRVDVVAPNMFAGSILDLDPNTEYEARFVLSDPDGVRGEAER
                     TSSVRTRAEPTRPTGGRTFHVYPHGFTGRKVEPAFEGLMCAYNEWCAGTDWATSGRPR
                     VRAGDTILVHGGTYQYNRYEYTNDAAVNRTTPLDGTYYFTADGTAERPIAIVAAGDGD
                     VIFDGNGNYALFDVRAADYTYFEGITFRNAEVGILAGTQFIAGSKGLTVKNSRFERVG
                     AGIFTNYSGSSGFYIADNYFVGRNDPRHLIGWAGDMWIKFRGVDGQIFPPAMASYVAV
                     KLYGPGHVVAYNYIADFHDGINVETYGNPDGSVASGPGIADGPKYPPREFFDRRPVAI
                     DFYNNYITNSHDNPIEADGSMHNIRVLRNMFINHASHAFCNQPTLGGPVYWIRNIAYH
                     LPAGSTRLTNGAAGVLFYNNTILSETVAAGTSNTHWRNNLILGENSAPEIFNVTTFTQ
                     YTSSDYNGFRPNPGAAASFRWSAPRAGVAADYNSFGHMAELTAREFATLEQYAAATGQ
                     DKNSVLVDYDVFMNVPQLD"
BASE COUNT          694 a         1310 c         1247 g          681 t
ORIGIN      
        1 taacgcgcat gggcatcgag aagagcatgc tcgaaaccgg cacgacgatt aaggtgcgcg
       61 gcaatgtcgg ccgccgcaat gcgcacgcgc tgtgggtctc gaacatcctg ctgccgaacg
      121 gcacggagct gctcgcggcg cccggcgcca caccgcattg gggctcgaag accgtcggcg
      181 atgcgacgga gttcttcgtc gccggcgacc tgaagctgcc ggccggcagc gagcgctcgt
      241 tcttccgcgt ctggtcgccg ctgatctcgg cgttcccgcg cccgcgcggc atgccggcgc
      301 tgaccgaggc ggggcaacgc gcgcaagctc gctacgagac gggcgagcaa gccgtcggcg
      361 actgcgaagt gcccggcatg ccgtacgcga tgatgagccc gtacccgatc gagatcgtgc
      421 aacaaggcaa ccggctgctg attcgcggcg aggcctacga cctcgtgcgc acggtggatc
      481 tcgccgcgcc ggcgaccacg cccgcgccga cgccgctcgg ctcgtcggtc gcgaaactgt
      541 caggcgacga gctcgtcatc gagacgagcc gcatcgacta tcacagctat ggcgatctcg
      601 ggcccgcgca gagcaaccaa agccacgtcg tggagcgctt caagctgtcg gcggacggta
      661 cgaagctcaa ttacgagatc acggtcaccg accccgtgat gctgcgtgag ccgtggctct
      721 ggggcggctc gttcgtctat cgcgaagggg ccgagatcaa gccgtggcgc tgcggcgccg
      781 accgcgcgta gcgccacgac cttccgcgat cacgccgact cgagcagccg ctcggggtcg
      841 atcttgtcgg cgaacacggc agcgctcgaa gcgtggcgcg tgtcgatcca ggcgcgaatg
      901 cgcgcgcttt caatcgtgcc gcccgtctcc gctaggtcgt cgacgaactc actcatgaac
      961 tgctcgtggt gccgcgccgc aaacgccgag cagtaaccgc ggaaacactt gacgggctcg
     1021 cgctcgtcga gcgcgccgcg ctgcggcgtg acgaacaaat tcaaaatgtt cagcgcgaga
     1081 tcgagacgct cgcgtcgcgt ggggcgtgac gtgttctctt cgggccgatg ctcgagaatg
     1141 accggctgac gaacgaccga ccgcgagatg ttggcatgta tggcgttcgc cacacgccat
     1201 agccggattt ccggaagtac gttgtgctga atgactgggt tcacggtaat ttccccatcg
     1261 ttgtttaccg aatgaaacaa accggcgtgc ctcgaggcgc gccgtctcat gccgtaacca
     1321 ggcggcgctc agtgggcggc ggtatcgcgg ccctggggcg gccgttcctc gcggcggctc
     1381 tcgcgtttcg ctccctcctg tttcgtggcc tcctcgcgct ttgccgcgtc ctgcggtctc
     1441 gcgccttcga agcgttcgtc ggtgcgctcg gcggcgcgcc ccacggcgtc tgtcaacggc
     1501 cgatcgtcac gcaagacgtc ggcccacgac gagtaaatcg tgcgaacctg ggtggcgtag
     1561 ttgtcggcga actgctccgt gagcctgtcg agccggcgca ccagcggctc gacatggccg
     1621 tcgaagctcg cgtaggccgc caagcggtcg ttcatgaagc gagtcgtcag ggccgccaac
     1681 tccacctgcc cgatcgcgat gcgcgttgcg ctgacgttca ttgcattcgc ggcgttccac
     1741 gcttgcagcc acggaccgct cactcgcgcg taggtttcca atccttcaac aatcattgtt
     1801 gccacctcca tggtgtgtct ttgagtgtta ctgcttggtc acgtgtcgga gactgccatg
     1861 catttcttac gctggcgttg cagtcgacac gcgcgacgtc cagcaaggtc tatgcccggg
     1921 tgtccttcgc ttctcgtcac gagcctcctc agagaggatc gaggagcgcg agttcaatat
     1981 tttgggacgg cacgttccaa atcttgtgcg gcatcgtgga gcgcttgtgg cgcgcagggg
     2041 cgggcgatcg ccgctgtggc acaattgcac tatgtcgacg atgtcgcttc gcctcggcgg
     2101 gactgcactg gccctcgttt gtgcggtgac tgcggtagcg caggcgccac aatcgagcgc
     2161 acgaagcaac gccgtcgtgc ccggcgagct cgtcgtcgag ccggcgacgc tgatcaacct
     2221 cggcttcgaa tggtggatcg agggcgatga caaccgcaac gcgagtgtcg ccgtctcgtt
     2281 tcgcaaacgc ggcgagagcg catggcagcc cgcgctgcca atgctgcggt tgcatggcga
     2341 gcgcgtttac agcgaatcga gagtcgacgt cgtcgcgccg aacatgttcg ccggcagcat
     2401 cctcgatctc gacccgaaca ccgagtatga agcgcggttc gtgctctcgg accccgacgg
     2461 cgtacgcggc gaagccgaac gcacgtcgag cgtgcgcacg cgcgcggagc cgacgcggcc
     2521 cacgggcggc cgcacattcc acgtctatcc gcatggcttc acgggacgca aagtcgagcc
     2581 cgcgttcgaa gggctgatgt gcgcttacaa cgagtggtgc gcgggcaccg actgggccac
     2641 gtcgggccgg ccgcgcgtgc gggccggcga cacgattctc gtgcacggcg gcacatatca
     2701 gtacaaccgc tacgagtaca cgaacgacgc cgccgtgaac cgcacgacgc cgctcgacgg
     2761 cacgtattac ttcacggcgg acggaacggc cgagcggccg atcgcaatcg tcgcggccgg
     2821 cgacggcgac gtcatcttcg atggcaacgg caattacgcg ctgttcgacg tacgcgcggc
     2881 cgactacacg tatttcgaag gcatcacgtt ccgcaacgcc gaggtcggga tcctcgccgg
     2941 cacgcaattc atcgcgggat cgaaaggcct aaccgtcaag aacagccgct tcgagcgcgt
     3001 cggcgccggc atcttcacga actactcggg ctcgagcggc ttctacatcg ccgacaacta
     3061 cttcgtcggc cgcaacgacc cgcggcatct gatcggctgg gccggcgaca tgtggatcaa
     3121 atttcgcggc gtcgacgggc agatcttccc gcccgcgatg gcgtcgtacg tcgccgtcaa
     3181 gctgtacggc ccgggccacg tcgtcgccta taactacatc gccgattttc acgacggcat
     3241 caacgtcgaa acgtacggca atcccgacgg ctcggtcgcg tcggggcccg gcattgctga
     3301 cggcccgaag tatccgccgc gtgaattttt cgaccgcagg cccgtagcga tcgacttcta
     3361 caacaactac atcacgaaca gccacgacaa cccgatcgaa gccgacggca gcatgcataa
     3421 catccgcgtg ctgcggaaca tgttcatcaa tcacgcgtcg cacgcgttct gcaatcagcc
     3481 gaccctcggc ggccccgtct attggatccg caacatcgcg tatcacttgc ctgccggctc
     3541 gacgcgtttg acgaacggcg ccgccggcgt gctgttctac aacaacacga tcctctcgga
     3601 gaccgtggcc gccggcacgt cgaacacgca ttggcgcaac aatttgatac tcggcgagaa
     3661 ctcggcgccc gagatcttca acgtcacgac gttcacgcag tacacgtcgt ccgactacaa
     3721 cggtttccga ccgaacccgg gcgcggcggc gtcgttccgc tggagcgcgc cgcgcgccgg
     3781 cgtcgccgcc gactacaaca gcttcgggca catggccgag ctcacggcgc gcgagttcgc
     3841 gacgctcgag caatatgccg ctgcgacggg tcaggacaag aacagcgtgc tcgtcgacta
     3901 cgacgtcttt atgaacgtgc cgcagctaga cg
//
LOCUS       DATLEE010000548         3845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_642807, whole genome shotgun sequence.
ACCESSION   DATLEE010000548 DATLEE010000000
VERSION     DATLEE010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642807"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VM692_11805"
     CDS             complement(<1..201)
                     /locus_tag="VM692_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ52899.1"
                     /translation="MRALLTVCRKELLETLRERRVLISLLLGPLFGPVIFTVMINMTV
                     SRNLSSADEVLEIPMLGAEQAPN"
     gene            complement(198..932)
                     /locus_tag="VM692_11810"
     CDS             complement(198..932)
                     /locus_tag="VM692_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUQ52900.1"
                     /translation="MIEVDNISKRFGAVQAVEGVSFAAQDGRITGLLGPNGAGKSTCL
                     RILSTVLKPDTGAARVGGIDLAAEPLAAGRTLGVLPHSSGLYQQLTGRENIEYYGRLH
                     GIAEAPLATRVDALIARLDLGAIADRKAKGFSQGERTKIALARALVHDPQHLLLDEPT
                     SGLDVMATRHLRDWLRELRAHGRCVLLSSHVMQEVEALVDDLVIIAAGRVTLTGTPAE
                     LAQRFPGRSLEEIFVEAVSGAERTAA"
     gene            complement(929..2251)
                     /locus_tag="VM692_11815"
     CDS             complement(929..2251)
                     /locus_tag="VM692_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ52901.1"
                     /translation="MPAVYARCGTLEVPLDPAMPDGAKIGLFVARLGAFSPEPKPDPL
                     LLINGGPGGSTVDLYLQLRGAFELARRDRDIILVDQRGTGRSAEGFACAVPDDLSLDT
                     AGPDELNRAIDACVAELDHDPRFYTTSVAVQDLDHVRTALGIEQWNVYGVSYGTRVAQ
                     HYLRRFPDRVRSVVLDGVVPPPLALGPDVAREAQRALEQIFARCGAEPQCGARFAALP
                     QLFAEVLTRLDAGASDETDPPPISGLELRTLIRFMSYNAATVALLPVLISEAHAGNYG
                     PLAGQARTLLRELPESLSFPMSNSVMCAEDAPYVTDSATGGLDATYLGTGIMDGINLI
                     CGRWPVGALDADFKAPVVSTTPVLLLSGEYDPITPPSYAERVKTEGLANSVHLIGRGQ
                     GHGMVGVGCVPRLLRSFLEKPVPAELDATCLEREPPTPFFLSLLGPAP"
     gene            2473..>3845
                     /gene="mnmG"
                     /locus_tag="VM692_11820"
     CDS             2473..>3845
                     /gene="mnmG"
                     /locus_tag="VM692_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HUQ52902.1"
                     /translation="MDYPTRFGCIVVGGGHAGTEAALAAARMGVRTLLVTQNLETLGQ
                     MSCNPAIGGIGKGHLTKEIDALGGAMARAIDRAGIHFRTLNGSKGPAVRATRAQADRA
                     LYKRAVRELLDAEPNLTLLQQNVDGLWLDGARVRGLVTQAGMRIEAETVVLTVGTFLD
                     GKIHVGPVSYSGGRAGDAASIGLARGLRDLGLAVGRLKTGTPPRIDGRTIDYTNLAEQ
                     PGDTPTPVFSFLGRRAEHPRQISCHITHTTERTHEIIRKGLDRSPLYTGAIEGVGPRY
                     CPSIEDKVVRFADRTRHQIFIEPEGLSTFEVYPNGISTSLPFDVQLELVHSIPGFENA
                     HLTRPGYAIEYDFFDPRDLQPWLETKAIANLFFAGQINGTTGYEEAAAQGIVAGINAG
                     LRVREREPWYPRRGDAYIGVLIDDLVSKGVTEPYRMFTSRAEYRLQLREDNADLRLTE
                     DGRRLGVV"
BASE COUNT          634 a         1315 c         1323 g          573 t
ORIGIN      
        1 gtttggggcc tgctcggcgc cgagcatcgg tatctcgagc acctcgtcgg ccgacgaaag
       61 attgcgcgag accgtcatgt tgatcatgac cgtgaagatc acggggccga acaacggccc
      121 gagcagcagc gagatcaaga cccgccgttc gcgcagcgtc tcgagcagct ccttgcggca
      181 cacggtcagc agcgccctca tgcggccgta cgctccgcgc cgctgacggc ttcgacgaaa
      241 atttcctcga ggctgcggcc cgggaagcgc tgcgcgagct ccgccggtgt gccggtcagc
      301 gtgacgcgcc cggcagcgat gatgacgaga tcgtccacga gtgcctcgac ctcctgcatg
      361 acatggctcg acaacagcac gcagcgtccg tgagcgcgca gctcgcgcag ccagtcgcgc
      421 aagtggcgcg tcgccatgac gtcgaggcca ctcgtcggct cgtcgagcag caggtgttgc
      481 gggtcgtgaa ccaaagcacg ggccagcgcg atcttcgtgc gctcgccttg cgagaagcct
      541 ttggctttgc ggtcggcgat tgcgccgaga tcgagccggg cgatgagcgc gtcgacgcgc
      601 gtcgcgagcg gcgcttcggc gatgccgtgc aagcggccgt agtactcgat gttctcgcgc
      661 cccgtcagct gttgatacag ccccgagctg tgcggtagga cgccgagcgt gcgccccgcc
      721 gcgagcggct cggccgcgag gtcgatgccg ccgacgcgtg ccgcgcccgt gtcgggcttc
      781 aacaccgtcg acaagatgcg caggcacgtc gatttgccgg cgccgttggg cccgagcaga
      841 cccgtgatgc ggccgtcttg cgccgcgaaa ctcacgccct cgacggcctg cacggcgccg
      901 aagcgcttgc tgatgttgtc gacctcgatc acggcgcggg cccgagcagg ctcaagaaaa
      961 acggcgtcgg cggctcgcgc tcgaggcagg ttgcgtcgag ctcggccggc accggcttct
     1021 cgaggaacga gcgcagcaga cgcggcacgc agccgacgcc gaccatgccg tggccttggc
     1081 cgcggccgat caggtgcacg ctgttggcga gcccttcggt tttgacgcgc tccgcataac
     1141 tcggcggagt gatcgggtcg tactcgccgg acagcagcag cacgggcgtc gtgctgacga
     1201 ccggcgcctt gaagtcggca tccagcgcgc cgacgggcca gcgcccgcag atcaggttga
     1261 tgccgtccat gatgccggtg cccaagtacg tggcgtcgag cccgcccgtc gccgagtccg
     1321 tcacgtacgg agcgtcctcg gcacacatca cggagttgct catcgggaag ctcagcgact
     1381 ccggcagctc gcgcaacagc gtccgggctt ggccggcgag cggcccgtaa ttgccagcgt
     1441 gcgcctcgct gatcagcacg ggcaacagcg cgacggttgc cgcgttgtag ctcatgaagc
     1501 gaatcaacgt gcgcagctcg agcccgctga tcggcggcgg atccgtctcg tccgacgcgc
     1561 cggcgtcgag gcgcgtgagc acctcggcga acagctgcgg cagcgcggca aagcgtgcgc
     1621 cgcactgcgg ctcggcgccg caacgcgcga agatctgctc gagcgcgcgc tgcgcctcgc
     1681 gtgcgacgtc ggggccgagc gccaacggcg gcggcacgac gccgtcgagc acgacggagc
     1741 gcacgcggtc ggggaaccgc cgcagatagt gctgcgcgac gcgcgtgccg tacgacacgc
     1801 cgtaaacgtt ccactgctcg atgccgagcg ccgtgcgaac gtggtcgaga tcctgcacgg
     1861 caacggaggt cgtatagaac cgcgggtcgt gatcgagctc cgcgacgcag gcgtcgatgg
     1921 cgcgattcag ctcgtcgggc cctgccgtgt cgagcgacag atcgtcgggc acggcacacg
     1981 cgaacccttc ggccgagcgg cccgtgccgc gctgatcgac gaggatgatg tcgcgatcgc
     2041 ggcgcgcgag ctcgaacgcg ccgcgcagct gcaagtacag atcgacggtg ctgccgccgg
     2101 ggccgccatt gatcaacagc agcgggtccg gtttcggctc gggactgaac gccccgagcc
     2161 gcgcgacgaa cagcccgatc ttcgctccgt ccggcatggc cgggtcgagc ggaacctcga
     2221 gcgtgccgca gcgtgcataa acggccggca acgaatcagt ggtgagccga cagcgatcgt
     2281 ccggaagcca cctcgaatct tccgcaacgc tgggttgcgc ggcggcgaaa gcgaggaggg
     2341 cgaccggcag caacggcagg cgaatgagca tagcggcgca tattcgcgca actaaccgtg
     2401 cccttgccag cggcaggact tggcgattct tggtggtcag ggccgcgtta cgccgcctag
     2461 aatcacgcct caatggacta tccgacccgc ttcggttgca tcgtcgtcgg cggcgggcac
     2521 gccggcaccg aggccgcgct cgccgcggcg cgcatgggcg tgcgcacgct actcgtcacg
     2581 caaaacctcg aaacgctggg tcaaatgagc tgcaacccgg cgatcggcgg tatcggcaaa
     2641 gggcatctga cgaaggagat cgacgcgctg ggcggagcca tggcgcgcgc gatcgatcgc
     2701 gccggcatcc acttccggac tttgaacggc agcaagggac cggccgtgcg cgcgacgcgc
     2761 gcgcaggccg atcgcgcgct ctacaagcgt gccgtgcgcg agctgctcga cgccgagccg
     2821 aatctcacgt tgctgcagca gaacgtcgac ggcttgtggc tcgacggcgc gcgcgtgcgc
     2881 ggcctcgtca cgcaggccgg catgcgcatc gaggccgaga ccgtcgtcct cactgtcggc
     2941 acgttcttgg acggcaagat ccacgtcggc cccgtcagct actccggcgg gcgcgccggc
     3001 gatgccgcgt cgatcggcct cgcgcgcggc ttgcgcgacc tcggcctcgc ggtcgggcgc
     3061 ctgaagaccg gcacgccgcc gcgcatcgac ggccgcacga tcgactacac gaatctcgcc
     3121 gagcagcccg gtgatacgcc gacgccggtg ttctcgttcc tcgggcgccg cgccgagcac
     3181 ccgcgccaga tcagctgtca catcacgcac acgaccgagc gcacgcacga gatcattcgc
     3241 aagggtctcg accgctcgcc gctgtacacc ggcgccatcg agggtgtcgg gccgcgctat
     3301 tgcccgtcga tcgaggacaa ggtcgtgcgg ttcgcggacc gcactcggca tcagatcttc
     3361 atcgagcccg aaggcctctc gacgttcgag gtctatccga acggcatctc gacgtcgttg
     3421 ccgttcgacg tgcagctcga gctcgtacat tcgatcccgg gtttcgagaa cgcgcacctg
     3481 acgcgccccg gctatgcgat cgagtacgac ttcttcgatc cgcgcgactt gcagccatgg
     3541 ctcgagacga aggcgatcgc gaatctgttc ttcgcgggtc agatcaacgg cacgacgggt
     3601 tacgaggaag ccgcggcaca aggcatcgtc gccggcatca acgccggttt gcgcgtgcgc
     3661 gaacgcgagc cgtggtaccc gcggcgcggg gacgcctaca tcggcgtgct catcgacgac
     3721 ctcgtctcca agggcgtgac cgagccgtat cgcatgttca cgagccgggc cgagtaccgg
     3781 ctgcagctgc gcgaggacaa cgctgatctg cgcttgaccg aggacggtcg tcgactcggc
     3841 gtggt
//
LOCUS       DATLEE010000549         5906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5110148, whole genome shotgun sequence.
ACCESSION   DATLEE010000549 DATLEE010000000
VERSION     DATLEE010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5110148"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VM692_11825"
     CDS             <1..361
                     /locus_tag="VM692_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUQ52903.1"
                     /translation="TIVPSEIKYEKNPAGSGPDLAVIAGDPTKEGFYIIRARFAPGVM
                     SMPHSHPGDRHVTVISGTWWAGKGPSGDPNATTPLGPGSYMLHPAGEVHYDGAKDVEA
                     IVEIKGMGPAPSIPYTR"
     gene            384..1649
                     /gene="soxC"
                     /locus_tag="VM692_11830"
     CDS             384..1649
                     /gene="soxC"
                     /locus_tag="VM692_11830"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HUQ52904.1"
                     /translation="MTNRTTLDLLEPVAGNGLLHRRLFLTGSAAAAGLTFLTARAAPP
                     DVPASMKTPGAGMRAYGERSPYEAKVQRTVGPTPGTVGTGGSRTPLEAVEGIITPSSL
                     HFERHHNGVPDINPDEHRVLIHGLVERPLIFSIDALARYPLVSRIQFLECSGNSAANN
                     NAEAPQQSAGGIHGLVSCSDWTGVPLAILLEEAGVKPEGKWLLAEGADAAAMSRSVPM
                     AKAMDDAVLALYQNGERLRPEQGYPVRLFLPGYEGNMNVKWLRRIKVTAEPTMTKDET
                     SKYSDLQPNGRSLLFTFPMEVKSVITSPSGTQKMQGPGLYQISGVAWSGFGRVRRVEV
                     SADGGETWAQAALDGAVLPRALTRFRMPWRWNGSPSVLQSRAIDETGAVQPSRPALMA
                     ARGASFRYHYNAIQSWNVAAAGEVSNVYA"
     gene            1642..2178
                     /locus_tag="VM692_11835"
     CDS             1642..2178
                     /locus_tag="VM692_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUQ52905.1"
                     /translation="MRNRTVAVAVAFAGAWLTCATAQEGPNLGVPATPEQVAGWDDSI
                     GPDGAGLPRGSGTAAAGKGVYEAKCLACHGVDGAGRPNDQLVGGQGTMRDAAPIRTIG
                     SYWPYATTVFDYIRRAMPLVAPHSLTADETYAVTAYLLALNGVIEQNAVMDATTLPKV
                     VMPNRANFDSAYREPRRR"
     gene            complement(2188..2544)
                     /locus_tag="VM692_11840"
     CDS             complement(2188..2544)
                     /locus_tag="VM692_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HUQ52906.1"
                     /translation="MRSIGLTILLLGLSNIFMTFAWYAHLKELNNRPWIVAALVSWGI
                     AFFEYMLQVPGNRIGYTVLSVGQLKLMQEVITLAVFVPFAVYYLKEPLKLDYLWAALC
                     ILGAVYFVFRGRLAGA"
     gene            2622..4436
                     /locus_tag="VM692_11845"
     CDS             2622..4436
                     /locus_tag="VM692_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018632887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HUQ52907.1"
                     /translation="MAAIAPDRPKGKSLKPLRALGPFLKPHWRVLAFALTALLVAAVA
                     QLALPIALRFLIDDGLAVRDAATINRYFVLFLIAAVVFGGFAALRYYLVTWLGERVVA
                     DMRNAVYANVIRMDPTFFEVTRTGEVLSRLTADTTLVQSISGVGISITLRSTLTLLGS
                     LVLLIATSPMLTLIIVVLLPLIVGPLIMLGRRLRGLSRDSQDRVADASALAGETINAV
                     QTVQAFNLEPLQARRFRDAVEHSFAVATRRNRLQGWLTALGTTFVFGAITFVLWIGAR
                     QVLAGTLTFGQLSQFLVYAGYLGLAGASLSEMWNQVQRAAGAMERLIELAEAEPTIKA
                     GPQPLDLPTASRGRIEFQHVRFRYPSRPESAALDDFTLTIEPGETVAFVGPSGAGKST
                     TFQLLLRFYDPAEGRVLIDGIDIARVNPEHVRKRIGLVPQDTVLFGASARENIRYGRP
                     DATDAEIEAAAAAAEADEFLRALPQGYDTFLGERGTRLSGGQRQRLAIARAILKDPPI
                     LLLDEATSSLDAASELLVQQALEKLMQKRTTIIIAHRLATVQKADRIVVIDRGRIVAS
                     GTHVELLAANPLYARLAELQFGAAGAAPEEKPRAALVR"
     gene            4417..5289
                     /locus_tag="VM692_11850"
     CDS             4417..5289
                     /locus_tag="VM692_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ52908.1"
                     /translation="MRLWSVEGNRQRLDGGAMFGNAPRNLWEKWLTPDDENRLEFACR
                     ALLVEDLDGRTVLFETGIGAFFSPKLKARYGVLEDEDVLLRSLAARGVAPEDVDVVVL
                     SHLHFDHAGGLLTAYEEGRAPELLFPRARFIVGKRAWERARAPHPRDRASYIPELPEL
                     LVRSARLEVIDRPQSTMLGPKVRFDFSDGHTPGLTLAEIVGKSGDGGVAFCSDLIPGR
                     PWVHLPVTMGYDRYPELLIDEKRRFLEDKIARNVRLFFTHDLECAVATPTRGADDRFG
                     VTAEQGALEGETLE"
     gene            5390..>5906
                     /locus_tag="VM692_11855"
     CDS             5390..>5906
                     /locus_tag="VM692_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52909.1"
                     /translation="MLHLKSLAVVVVLSVAVGARAQITENPIKEPIVKRGLAVEVRDV
                     VRLPETRNLRPVDQDVTPTGWARVSFVRDLPDGRRFANDSRGFLYLVEGDKTSLYADV
                     AAAFPRAWYARLESGFIGFDFHPDFANNGLWYSVHLERGKDNPAKPDFIPPGYDADDV
                     TFHNVITEWKAN"
BASE COUNT          962 a         1951 c         1990 g         1003 t
ORIGIN      
        1 caccatcgtg ccgagcgaga tcaagtacga gaagaatccg gccggcagcg gtccggacct
       61 cgccgtgatt gccggcgacc cgacgaagga aggcttctac atcatccgcg ctcggttcgc
      121 gccgggcgtg atgtcgatgc cgcactcgca ccccggcgat cggcacgtca ccgtcatttc
      181 cggcacttgg tgggccggca aagggccctc gggcgatccg aacgccacga cgccgctcgg
      241 ccccggcagc tacatgctgc atccggccgg cgaagtgcac tacgacggcg cgaaggacgt
      301 cgaggccatc gtcgagatca aagggatggg accggcgccg tccattccgt atacccgctg
      361 aagcgcccgc aaggactccg tcgatgacga atcgaactac gctcgacttg ctcgagcccg
      421 tggccggcaa cggtctgctg catcgccgct tgttcctgac gggaagcgcc gcagcggcag
      481 ggctcacgtt cctgaccgcg cgcgcggcac cgcccgacgt gcccgcatcg atgaaaactc
      541 ccggcgcagg catgcgcgcg tacggcgagc gctcgccgta tgaggcgaag gtgcagcgca
      601 cggtcggccc gacgcccggc acggtcggca cgggcggctc gcggacgccg ctcgaagccg
      661 tcgaaggcat catcactccg agctcgctgc atttcgagcg gcaccacaac ggcgtccccg
      721 acatcaaccc cgacgagcat cgagtgttga tccacggact cgtcgagcgg ccattgattt
      781 tcagcatcga cgcgcttgcg cgttatccgc tcgtgtctcg catccagttc ctcgagtgct
      841 cgggcaacag cgctgccaac aacaacgcgg aagcgccgca acaatcggcc ggcggtattc
      901 acggcctcgt ctcgtgcagc gattggaccg gcgtgccgct tgcgattctg ctcgaggagg
      961 ctggcgtcaa gccggaaggc aagtggctgc tcgcggaggg cgccgatgcg gccgcgatga
     1021 gtcgcagcgt gccgatggcg aaagccatgg acgacgcggt gctcgcgctc tatcagaacg
     1081 gcgagcgttt gcggccggag caaggctatc ccgtgcgcct gttcctgccg gggtatgagg
     1141 gcaacatgaa cgtgaagtgg ctgcgccgca tcaaggtgac ggccgagccg acgatgacga
     1201 aggacgagac gtcgaaatac tccgacttgc agccgaacgg ccgctcgctg ctgttcacgt
     1261 tcccgatgga agtgaaatcc gtgatcacgt cgccgtcggg cacgcagaag atgcaggggc
     1321 ccgggctcta tcagatctca ggcgtcgctt ggtccgggtt cggccgtgtt cgccgcgtcg
     1381 aggtgtctgc cgacggcggc gagacgtggg cgcaggccgc gctcgacggg gcggtgctgc
     1441 cgcgcgctct tacgcgcttc cgcatgccat ggcgctggaa tggcagcccg agcgtgctgc
     1501 agagccgagc gatcgacgag accggtgccg tgcagccgtc gcgccccgcg ttgatggccg
     1561 cgcgcggtgc atcgttccgt taccactaca acgcgattca aagctggaac gtcgcggccg
     1621 ccggcgaggt cagcaacgtc tatgcgtaac cgaactgttg cagtcgcggt tgcgttcgcc
     1681 ggcgcctggc tcacgtgcgc cacggcgcag gaagggccga atctcggcgt gcccgcgacg
     1741 cccgagcaag tcgccggttg ggacgacagc atcgggcccg acggcgccgg cttgccccgc
     1801 gggtcgggca ccgcggccgc gggcaagggc gtgtacgaag cgaagtgctt ggcgtgccac
     1861 ggtgtcgacg gcgcgggccg gcccaacgat cagctcgtgg gcgggcaggg cacgatgcgc
     1921 gacgctgcgc cgattcgcac gatcggcagc tactggccgt acgcgacgac ggtgttcgac
     1981 tacatccgcc gcgcgatgcc gttggttgcg ccgcactcgt tgacggccga cgagacgtac
     2041 gcggtcacgg cgtatctgct cgccttgaac ggcgtcatcg agcagaacgc cgtcatggac
     2101 gcgacgacgc tgccgaaggt cgtcatgccg aatcgcgcca acttcgactc ggcgtatcgc
     2161 gagcctcgcc ggcgctgagc cggagcgtca cgcgccggcc aagcggccgc ggaatacgaa
     2221 gtagacggcg ccgagaatgc acagcgccgc ccacaagtag tcgagcttca gcggctcctt
     2281 caagtaatag accgcgaacg gcacgaacac cgcgagcgtg atgacctctt gcatgagctt
     2341 cagctgaccg acggacagca cggtgtagcc gatgcgattg cccggcacct gcagcatgta
     2401 ctcgaagaac gcgattcccc agctcacgag cgccgccacg atccacggcc tgttgttgag
     2461 ctctttcaaa tgcgcgtacc acgcgaacgt catgaagatg ttgctcaagc cgagcaagag
     2521 gatcgtcagg ccgatactgc gcatcgtgaa atttccgtaa tccgcgacgg ccgcgagtat
     2581 cgcacgcgtc tgcggccgtc tcattcgcgt taagcttggt catggccgcc atcgctcccg
     2641 accgccccaa aggtaaatcg ttgaagccgc tgcgcgcgtt ggggccgttc ctcaagccgc
     2701 attggcgcgt gctcgcgttc gcgttgacgg cgctgctcgt cgcggccgtc gcgcagctcg
     2761 ctttgccgat cgcgctgcgc ttcttgatcg acgacggtct cgccgtccgc gatgccgcga
     2821 cgatcaaccg ctatttcgtt ctgttcctca tcgcggccgt cgtgttcggc gggttcgcgg
     2881 cgttgcgcta ctacctcgtc acgtggctcg gcgagcgcgt cgtcgccgac atgcgcaacg
     2941 ccgtgtacgc gaacgtgatc cggatggatc caacgttctt cgaggtcacg cggaccggcg
     3001 aggtgctgtc gcggctcacg gccgatacga cgctcgtgca atcgatctcg ggcgtcggca
     3061 tctcgatcac gctgcgctcg acgctgacgc tgctcggctc gctcgtgctg ctgattgcca
     3121 cgagcccgat gctgacgttg ataatcgtcg tgctgctgcc gctgatcgtc gggccgttga
     3181 tcatgctcgg taggcgtttg cgcgggctgt cgcgcgactc gcaggaccgc gtggcagacg
     3241 cgagcgcgct cgccggcgaa acgatcaacg ccgtgcagac cgtgcaggcg ttcaatttgg
     3301 agccgttgca agcgcggcgg tttcgcgacg ccgtcgagca ctcgttcgcc gtcgcgacgc
     3361 ggcgcaatcg tctacagggc tggctgacgg cgctcgggac gacgttcgtg ttcggcgcca
     3421 tcacgttcgt gttgtggatc ggcgcgcgtc aggtgttggc cggcacgctg acgttcgggc
     3481 agctcagcca attcctcgtc tacgcgggct acttggggct cgccggcgct tcgctgtcgg
     3541 agatgtggaa ccaagtgcag cgcgcggccg gcgccatgga gcggctcatc gagctcgccg
     3601 aggccgagcc gacgatcaag gcagggccgc agccgctcga cctgccgacc gcgagccgcg
     3661 gccgaatcga gttccagcac gtgcgcttcc gttatccgtc gcgccccgag agcgcggcgc
     3721 tcgacgactt cacgttgacg atagagcccg gcgagaccgt ggcgttcgtc ggcccttccg
     3781 gtgccggcaa gagcacgacg ttccaactgc tgctgcgctt ctacgatccc gcagaggggc
     3841 gcgtgttgat cgacggtatc gacatcgcac gcgtgaaccc ggagcacgtc cgcaagcgga
     3901 tcggcctcgt gccgcaggac acggtgctgt tcggcgcgtc ggcgcgcgag aacattcgct
     3961 acgggcgccc cgacgccacg gacgcggaga tcgaagccgc cgccgcggct gccgaggccg
     4021 atgagttcct gcgtgctttg ccgcagggct acgacacgtt cctcggcgag cgcggcacgc
     4081 gtctgtcggg tggacagcgg cagcggcttg cgatcgcgcg cgcgatcctc aaggatccgc
     4141 cgattctgct gctcgacgaa gcgacgagct cgctcgatgc ggccagcgag ctgctcgtgc
     4201 agcaggcgct cgagaagctc atgcaaaagc gcacgacgat catcattgcg caccgtctcg
     4261 cgaccgtgca gaaggccgat cgcatcgtcg tcatcgatcg cgggcggatc gtcgcgagcg
     4321 gcacgcacgt ggagctgctc gccgcaaatc ccctgtacgc gcggctcgcc gagctgcaat
     4381 tcggtgcggc cggcgccgct ccggaggaga agccgcgtgc ggctctggtc cgttgaaggc
     4441 aatcgccagc gtctcgatgg cggcgcgatg ttcggcaatg cgccgcgcaa cctgtgggaa
     4501 aagtggctga cgcccgacga cgagaatcgg ctcgagtttg cctgccgcgc attgctcgtc
     4561 gaggatctcg acggccgcac ggtgctgttc gagaccggca tcggcgcatt cttcagcccg
     4621 aagctcaaag cgcgctacgg cgtgctcgag gacgaggacg tcttgctgcg ctcgcttgcg
     4681 gcgcgcggtg tcgcgccgga ggacgtcgac gtcgtcgtgc tgagccacct gcacttcgat
     4741 cacgcgggcg ggttgctgac ggcgtacgaa gaagggcgcg cgcccgagct gttatttccg
     4801 cgcgcgcgtt tcatcgtcgg caaacgtgcc tgggagcgcg ctcgcgcgcc gcatccgcgc
     4861 gaccgtgcgt cgtacatccc ggagctgccg gagctgctgg tacgaagtgc gcgtctcgag
     4921 gtcatcgacc ggccgcagtc gacgatgctg ggccccaaag tgcgcttcga cttcagcgac
     4981 ggccatacgc cgggcctcac gcttgcggag atcgtcggaa agagcggcga cggcggcgtc
     5041 gcgttctgct ccgatctgat cccggggcgg ccgtgggtgc atctgcccgt cacgatgggt
     5101 tacgaccgct atcccgagct cttgatcgac gagaagcgcc gcttcctcga ggacaagatc
     5161 gcgcgcaatg ttcgcttgtt cttcacgcac gatctcgagt gcgccgttgc gacgccgacg
     5221 cgcggcgccg acgaccgctt cggcgtcacg gccgagcagg gcgccctcga gggcgagaca
     5281 ttggaatgat cacgcgcgag ctcgccgagc actatcaata gcggcttgac cgtgcttggc
     5341 agcgcgggcg attcttcagg tcggcgcgca atccaattct gggggaaata tgcttcatct
     5401 gaagtcgttg gccgtcgtcg tcgtgctgtc ggtcgcggtc ggtgctcgcg ctcagatcac
     5461 cgagaacccg atcaaggagc caatcgtcaa gcgcggcctt gccgtcgaag tgcgggacgt
     5521 cgtgcggctt cccgagacgc gcaacttgcg gcccgtcgat caggacgtca cgccgacggg
     5581 ttgggcgcgc gtcagcttcg tgcgcgatct gccggacggc cggcgcttcg ccaacgactc
     5641 acgcggcttc ctttatctcg tcgagggcga caagacctcg ttgtacgcgg acgtcgcagc
     5701 ggcgttcccg cgggcctggt atgcgcgtct cgagagcggc ttcatcggct tcgatttcca
     5761 cccggacttc gcgaacaacg gcttgtggta cagcgtccat ctcgagcgcg gcaaagacaa
     5821 tcccgcgaaa ccggatttca ttccgccggg ttacgacgct gacgacgtga cgtttcacaa
     5881 cgtcatcacg gagtggaaag ccaacg
//
LOCUS       DATLEE010000550         3543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10759521, whole genome shotgun sequence.
ACCESSION   DATLEE010000550 DATLEE010000000
VERSION     DATLEE010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10759521"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VM692_11860"
     CDS             complement(<1..1115)
                     /locus_tag="VM692_11860"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HUQ52910.1"
                     /translation="MPGAGAARLSSRLTRVKPSSTLAMTSKVLELQQRGVNVIGFAAG
                     EPDFPTWRHVCEAAHDAIRAGEFRYTAVGGTPKLKQAIRTKLERDNGLVYADSEVVAS
                     CGGKHSLYNAMQAVLDEGDEVVIPGPFWVSYPDMAALSGGVAKIVSASEHNGFLLTDG
                     ELEAAITPRTRLVILNSPSNPTGATYSEQQIAALGRVLARHSCWVIVDDVYERIRYDG
                     TQPRHLLALEPSLRDRTIICNSVSKTYAMTGWRIGYTAAPPAVIKAITTLQSQSTSNP
                     SSIAQAAAAAALAGPQEELEPMVAEFRRRRDYICERVNAIDGLSVGVPGGAFYVFING
                     AGVFDKAGVTDGDALAMKLLEGAEVGVVGGNDFGSND"
     gene            complement(1117..1611)
                     /gene="coaD"
                     /locus_tag="VM692_11865"
     CDS             complement(1117..1611)
                     /gene="coaD"
                     /locus_tag="VM692_11865"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HUQ52911.1"
                     /translation="MNSRFAVYPGSFDPMTNGHLDILRRALTVFDEVRIAVAYNQDKP
                     SGLFTPLERVTIIKEVVSEFGSRVSADAFHGLLTDYCRRIGAGTIIRGLRAVADFEYE
                     FQMTMMNRHLTADVETVFLMAGEQHFYTSSRLVKEVASLGGDVHGLVPDAVEPHLLTR
                     VGRA"
     gene            complement(1644..2375)
                     /gene="rsmD"
                     /locus_tag="VM692_11870"
     CDS             complement(1644..2375)
                     /gene="rsmD"
                     /locus_tag="VM692_11870"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HUQ52912.1"
                     /translation="MRVIGGTFGGRRLRAVEGHGTRPTSDRVREALFSRIEARYGLSG
                     SRVLDMFAGTGALAIEALSRGAARAVCIESERRALETLRENVRELALAGRVRVIGDDF
                     RRVLARLKSFDGVFIDPPYGKGLALDALGAVAELALVRVGGWVTVEVGRREQMPEVVE
                     NVRDVPADAANDDAAEVAQAAATEIVADPAVVLRRIREDSYGDTKLVLYEAEGPARHD
                     RPTVAGLAPAAGGVDETGTRFAPAI"
     gene            2478..3146
                     /gene="cofC"
                     /locus_tag="VM692_11875"
     CDS             2478..3146
                     /gene="cofC"
                     /locus_tag="VM692_11875"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HUQ52913.1"
                     /translation="MNAALIPVRSIAGAKQRLAGCLDDARREELALAMLEDMLAALSA
                     ATRVDRIVVVSSDADLLRHARRCGAEALDEGPSRGLNGAVAFAAAELEAQGVTRLLVI
                     PGDVPLLDPFEIDALFEIDAARYPVVAVPSSSATGTNALVTSPPTAITFRFEGASLDA
                     YRRECRDRKLEMLILCLEGFAIDIDTPADLAALPSRGGYRTCELVDEWRDSFDNTSEA
                     GTGS"
     gene            3169..>3543
                     /locus_tag="VM692_11880"
     CDS             3169..>3543
                     /locus_tag="VM692_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52914.1"
                     /translation="MTSRLRALTLFLACAIPILALWLSVGWRAPEPYEIQWTDLPWYF
                     TPTFAVLHRALVEGFFPLWNPWQLAGWPFLAAFQTQVLYAPAWAVAWLPPHLAVTAYN
                     AIHWSWAFAGMSAFAAGIGLSAP"
BASE COUNT          582 a         1225 c         1111 g          625 t
ORIGIN      
        1 tgatcgttgc tgccgaagtc gttgccgccg acgacgccca cttccgcgcc ttcgagcagc
       61 ttcatcgcaa gcgcgtcgcc gtccgtgacg ccggccttgt cgaacacgcc ggcgccgttg
      121 atgaacacgt agaacgcgcc gcccggcacg ccgaccgaaa gcccgtcgat cgcattgacg
      181 cgctcgcaga tgtagtcgcg ccgcctgcga aattccgcga ccatcggctc gagctcttcc
      241 tgcggcccgg caagcgctgc agcggccgcc gcctgtgcga tcgagctcgg attggacgtg
      301 gactggctct gcagtgtcgt gatcgccttg atgacggccg gcggcgccgc ggtgtagccg
      361 atccgccagc cggtcatcgc gtacgtcttg gacaccgagt tgcagatgat cgtgcgatcg
      421 cgcagcgacg gctcgagcgc aagaagatgc cgcggctgcg tgccgtcgta acggatgcgc
      481 tcgtagacgt cgtccacgat gacccagcac gaatgccgcg cgagcacgcg gccgagcgcg
      541 gcgatctgct gctccgaata cgtcgcgccg gtcggattgg acgggctgtt caggatgacg
      601 agccgcgtgc gcggtgtgat ggcagcttcg agctcgccgt cggtaagaag gaaaccgttg
      661 tgctcgctcg cgctgacgat ctttgcgacg ccgcccgaaa gggcggccat gtcgggatag
      721 ctgacccaga acggaccggg gatgacgacc tcgtcgcctt cgtcgaggac ggcctgcatc
      781 gcgttgtaca gcgaatgctt tccgccgcag ctcgcgacaa cttcgctgtc tgcgtagacg
      841 agcccgttgt cgcgctcgag cttggtgcgt atcgcctgct tcagcttcgg cgtcccgccg
      901 acggccgtgt agcggaattc gccggcgcga atcgcgtcgt gcgcggcttc gcagacgtga
      961 cgccatgtcg gaaaatccgg ttcgcccgca gcaaacccga tcacgttgac gccgcgctgc
     1021 tgcagctcga gaacttttga cgtcatcgcg agcgtggatg acggcttgac gcgcgtcagg
     1081 cggctcgaca gacgtgcggc gcccgcgcct ggcatgctac gcgcgtccga cacgggtcag
     1141 cagatgcggc tcgaccgcat cgggaacgag cccgtgcacg tcgccgccaa gggacgcaac
     1201 ttccttgacc aggcgcgagc tggtgtagaa gtgctgctcg cccgccatca gaaacaccgt
     1261 ctcgacgtcg gccgtcaggt gacggttcat catcgtcatc tggaactcgt actcgaagtc
     1321 cgccactgcg cgcaggccgc gaatgatcgt gccggcaccg atgcggcggc aatagtccgt
     1381 cagcagaccg tggaacgcgt ccgccgaaac acgcgatccg aattcgctga caacttcctt
     1441 tataattgtg acgcgctcga gcggtgtgaa cagaccggac ggcttgtcct ggttgtaggc
     1501 gacggcgatg cgaacttcgt caaagaccgt cagcgccctg cgcaggatgt cgagatgacc
     1561 gttggtcatc ggatcgaacg agcccggata aacggcaaag cgtgaattca tccttcgatc
     1621 tcccacacca ctggccttac ggcctatatg gccggcgcga acctggtgcc ggtctcgtct
     1681 acgccgccgg ccgccggagc gagaccggca accgttggcc ggtcgtgcct ggccggaccc
     1741 tctgcctcat acaacaccag cttcgtatcg ccgtacgagt cctcgcgaat ccgtcgcagc
     1801 acgaccgccg gatcggcaac gatctcggtc gcggccgcct gcgcaacttc tgccgcgtcg
     1861 tcgtttgcag cgtccgcggg aacgtcgcgg acgttctcga cgacttccgg catctgctca
     1921 cgccggccga cctccaccgt cacccagccg ccaacccgga ccagcgcaag ctcggccacc
     1981 gcaccgagcg cgtccagtgc aagccccttt ccgtacggcg gatcgatgaa aacgccgtcg
     2041 aaacttttca gcctcgcaag cacgcgtcga aaatcgtcgc cgatcacgcg aacacggcct
     2101 gccagcgcca gctcgcgaac attttcacgc agggtctcga gtgcacggcg ctcgctttcg
     2161 atgcacaccg cccgtgccgc tccgcggctg agcgcttcaa tcgccagcgc gcccgtaccg
     2221 gcaaacatgt cgagcacgcg cgaccccgac aacccgtagc gagcttcgat gcggctgaac
     2281 aacgcctccc gaacgcgatc gctggtgggc cttgtcccat gcccttcgac cgcccgcaga
     2341 cggcggccgc cgaaggttcc accgatgact cgcattcacg tctctgccgg agagcggcgc
     2401 gttatagccg atcgatggca ggcgggcaaa aaagtccggc ctttgcctgc catcggggtc
     2461 tcacctacgg tcgctcgatg aacgccgctc tcatcccggt gcgctcgatt gccggggcca
     2521 aacagcgact ggccggctgt ctcgacgacg cgcgccgtga agagctcgcg ctcgcgatgc
     2581 tcgaagacat gctcgccgcg ctgtcggcgg cgacgcgcgt cgatcgcatt gtcgtcgtca
     2641 gctcggacgc cgatctgctg cgccatgcgc gccgctgcgg cgccgaagcg ctcgacgaag
     2701 gtccgtcgcg cgggctcaac ggcgcagtcg cgttcgccgc ggccgagctc gaagcacagg
     2761 gtgtgacacg ccttctcgtc attcccggcg acgtgccgct gctcgatccg tttgaaatcg
     2821 acgcgctgtt cgagatcgat gcggcgcgct acccggtcgt tgcagtgccg tcgtcgtcgg
     2881 caacaggcac caacgcgctc gtcacgagcc cgccgacggc gatcacgttt cgcttcgaag
     2941 gcgcaagcct cgacgcgtac cgccgcgagt gccgcgaccg caagctcgag atgctgatcc
     3001 tgtgcctcga aggtttcgcc atcgatatcg acacgcccgc cgatctcgcg gcgctgccga
     3061 gccgcggcgg ctaccgcacc tgcgagctcg tcgacgagtg gcgcgactcg ttcgacaaca
     3121 cctcggaggc cggcacgggc tcctgaaccg caacgcactc gtcccatcat gacatcccga
     3181 cttcgtgcgc tgacgctttt tctcgcctgc gcgatcccga tccttgcgct gtggctgagc
     3241 gtcgggtggc gtgcgccgga gccgtacgaa atccaatgga cggatctgcc gtggtacttc
     3301 acgccgacgt tcgcggtgct tcaccgcgcg ctcgtcgaag gcttctttcc gctgtggaat
     3361 ccgtggcagc ttgcaggctg gccgtttctc gccgcgtttc agacgcaggt gctgtacgcg
     3421 ccggcgtggg cagttgcgtg gcttccgccg cacctcgcgg taacggcgta caacgcgatc
     3481 cactggtcat gggcatttgc aggaatgtct gcgttcgcag caggcatcgg tctttctgca
     3541 ccc
//
LOCUS       DATLEE010000551         6317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10759846, whole genome shotgun sequence.
ACCESSION   DATLEE010000551 DATLEE010000000
VERSION     DATLEE010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10759846"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VM692_11885"
     CDS             complement(<1..536)
                     /locus_tag="VM692_11885"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HUQ52915.1"
                     /translation="MGTRLPQAKVTPRAPQDEILTGLVGRHIGASRSPWLHEREAQAQ
                     GLALTYKLLDFAVLGRDESDLCAQLDAAAEQGYAGLNVTYPYKQAVIRCLDELAPSAA
                     RVGAVNTVKFAAGKRIGHNTDVIGFAQSMRDGLPNAGLRCVLQIGAGGGGAATAQALL
                     ESGVATLFVYDRDRARAA"
     gene            complement(611..3499)
                     /locus_tag="VM692_11890"
     CDS             complement(611..3499)
                     /locus_tag="VM692_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ52916.1"
                     /translation="MSSTVGLTLRSSIIAALGVSATAGAQNAAGQAPNVEEIVVTGSS
                     LRGVAPVGSNLVTVGRGEIENTGAQTVQQILKTVPSVVGLQSAGQAAYGSFDGAGTNA
                     PTIHGLGASASNSTLILLNGHRMPVSGINHVLADPNIVAPLALERVEVLADGASSVYG
                     SDAVAGVVNFITRRNVDGIEASVQTGAADGYGTVNAGVLGGTTWSGGSLLVSYNYSDR
                     DNLDAVDRDYVRANQTARGGRDFTPNRCGPASITVAGQTYYTPYTAAGAIAGDCDPSL
                     HADLLASERRHSVFATIEQDVTARLTLTGDLIYSQRENDQAVPRGNASATMFGPGAAN
                     AAQINPFFRLPTGVAAGTSSIAVNFSGDALLGPGAHIESDVDTVYGRFDADYELTDTW
                     SFNAGFLIGLDSSRQQNFGQLCGSCFNLAINGTTNGGGSTTAASIPGTTTVVLNTPLT
                     AANALDPFANGTSDAVRAALMDSGQIQVGDQVIKNLYFKFDGDLFDMRGGAAQLAVGG
                     ELIDYELDQDIVRPNNTGPASTGSAQLFIPYARDVTSAYAELYLPFVGPEQDVPGVNS
                     FAVNLSTRYDDYSDVGSTTNPKIAFNWGIVEALMLRANYAEAFVAPALTSRGANQFGL
                     TGESGFSGIAGQGLAGGPPTIATASFPSAIGIPGCPAGSATCSLNNVTGLLLTGGSGT
                     LQPQTGKSWSLGVDITPPNVQGLRVSLTKWTNELRGGITAPVPSLALGSADLSSLIQF
                     FPTGATAAQIAAAAAGLPQTGALNASTFFFYNFQQRNVLNLDVAGLDVAVSYEFDAGG
                     GSMNVGAALTHKTKFDQFFGANGTKFSVLGTAGFNTTFPSVEDEGRLSFGYEREMFSI
                     NAYYNYLGSYRNWSGTAAIQPTRVAGLPSGGGGDPVDSFATVDVNVAFQLEKMELFLD
                     ALNLFDEEPPQYNTFAIGGNNGVGVAGYDPINASPLGRVLTVGLRARF"
     gene            complement(3627..4898)
                     /locus_tag="VM692_11895"
     CDS             complement(3627..4898)
                     /locus_tag="VM692_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ52917.1"
                     /translation="MTRTRARFGVLTLITLATMLNYVDRSVMGVAQPSLVRELAIGPE
                     VMGMIFSAFSWTYALAQIPGGLVLDKLGARLTYGLSLASWSLATVMHGLMSSVAGLFS
                     ARLALGIAEAPCFPANSRILSTWFPQAERARANGVYSVGQYVGLGFLIPVLTYIVALF
                     GWRALFFIFGGVGIVYAFAWYWLYREPHESASVNQAELDYIAAGGGLASGAGGFALSW
                     VAVRQLLRQRQIVGASIGQFCGNSTLVFFLTWFPSYLATERGMDWIRSGFAASLPYIA
                     ASVGVLVGGAISDGIIRRTGSATLGRKLPIITGLLLTSTMVLANFTPSNSLVIAIMSV
                     AFFGQGMVNLGWTLIADVAPKHLMGLTGGVFNFCANLAGIATPLVIGFVLGATGSFYG
                     ALAYIAALGLIGAAAYVFIVGDVRRVEIAAG"
     gene            complement(4895..5350)
                     /locus_tag="VM692_11900"
     CDS             complement(4895..5350)
                     /locus_tag="VM692_11900"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011537685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HUQ52918.1"
                     /translation="MRTTVYVFNGPNLNLLGQREPEIYGRDTLAEVEARCRALTDARG
                     LKLEFRQTNAEHVMVDWFQEARLGAVGIAFNPAAFTYAGYPILDAIKMVDCPVVEVHI
                     SNIHRREAEWRSKSIMTQVVTGIISGLGVHGYELAVQHLLQRHAAGTRA"
     gene            complement(5384..>6317)
                     /locus_tag="VM692_11905"
     CDS             complement(5384..>6317)
                     /locus_tag="VM692_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HUQ52919.1"
                     /translation="LLQASPPARGLFHKAVGMSGSPFSEPLTAVTLEQGEAAGLELQR
                     AVGAQSIEQLRDVGGDRIATAPGAREPIVLDGHYVIGAAKAFESKQQSDVPIMLGFTR
                     DESFRSLGRVTSVAELETAVRRVFPSTASAVLAAYPAKDASSAARAAADLGRDSSVGA
                     QMANWARTQAKFSRSPVYAYFFTRRQPYAPGITFIDHDPATAGAYHSGEIPYFLRTRD
                     SLNLFRQTRNWEEVDVKLEKDMAALLVSFARDGKPRSARVHDWPVFEPGRPRVVSLGT
                     EITVVDWPNYASLDLLQAPAAERPPAPPGNRPRD"
BASE COUNT         1142 a         2085 c         2045 g         1045 t
ORIGIN      
        1 atcgccgctc gagcacgatc gcggtcgtag acgaacagcg tcgcgacgcc ggactcgagc
       61 agcgcttgag ccgtcgcggc gccgccgccg ccggcgccga tctgcagcac gcaccgcagg
      121 ccagcgttcg gcaaaccgtc gcgcatgctc tgcgcgaacc cgatgacgtc cgtgttgtgc
      181 ccgatacgct taccggcagc gaacttgacc gtgttgacgg cgccgacgcg cgcggcgctt
      241 ggcgccaact cgtcgaggca gcggatcacg gcttgcttgt acggataggt cacgtttagc
      301 ccggcgtagc cttgctccgc cgccgcgtcg agttgcgcgc acaagtcgga ctcgtcgcgg
      361 ccgagcacgg cgaaatcgag aagtttgtag gtcagcgcga ggccctgagc ttgcgcctct
      421 cgttcgtgca gccacggcga gcgggacgcg ccgatgtggc gtcccacgag ccccgtgagt
      481 atctcgtcct gcggagctcg tggcgttacc ttggcctgcg gcaatctagt tcccaagttc
      541 acgtgcggcg acgaccttag ctaggcctgc gcgggtcgat ggggaccggc gcacttcgtt
      601 cctatcgatg ctagaaccgt gcacgcagtc caacggtgag cacgcggccc aacgggctag
      661 cattgatcgg atcgtagcct gccacgccga caccgttgtt gccgccgatc gcaaaagtgt
      721 tgtactgcgg cggctcctcg tcgaacaggt tcaacgcgtc gagaaacagc tccatcttct
      781 caagttggaa cgcgacgttg acgtccacgg tggcgaacga atcgacgggg tcgccgccgc
      841 cgccactcgg caggccggcc acgcgcgtcg gctgaatagc cgcggtgccg ctccaattgc
      901 ggtagctccc gagataattg tagtacgcgt tgatgctgaa catctcgcgc tcataaccga
      961 agctcagtcg gccttcgtcc tcgaccgacg ggaaagtcgt gttgaacccg gccgtgccga
     1021 gcacgctgaa cttcgtgccg ttcgcgccga agaactgatc gaacttggtc ttgtgcgtca
     1081 gagccgcacc aacgttcatc gagccgccgc cggcgtcgaa ctcgtagctg acggcaacgt
     1141 cgagccctgc aacgtcgagg ttcagcacgt tgcgctgttg gaagttgtag aagaagaagg
     1201 tgctggcgtt cagcgcgccc gtttgcggca agccggcggc agcggcggcg atctgcgcgg
     1261 cggtcgcgcc ggtcgggaag aactggatca agctactcaa gtcagccgag ccgagcgcca
     1321 gcgatggcac cggcgccgtg atgccgccgc gtagctcgtt ggtccacttc gtgaggctga
     1381 cccgcaagcc ctgcacgttg ggcggcgtga tgtcgacgcc gaggctccag ctcttgcccg
     1441 tctgcggctg tagcgtcccg ctgccgcccg tgagcaagag tcccgtcacg ttgttcagcg
     1501 agcacgttgc cgagcctgcc gggcatcccg ggattccgat agcggagggg aagctcgccg
     1561 tcgcgatcgt cggcgggccg cctgccagtc cttggcccgc gatgccgctg aaacccgatt
     1621 cgcccgtaag gccgaattgg ttcgcgccgc gactcgtcag cgcgggcgct acgaacgctt
     1681 ccgcgtagtt ggcccgcagc atcagcgcct cgacgattcc ccagttgaat gcaatcttcg
     1741 gattggtcgt cgagcccacg tcgctatagt cgtcgtagcg cgtcgacagg ttgactgcga
     1801 agctgttgac tcccggcaca tcttgctcgg gaccgacgaa cggtaggtac aactccgcgt
     1861 acgccgacgt gacgtcgcga gcgtagggaa tgaacagctg cgccgagccc gtcgaggccg
     1921 ggcccgtgtt gttggggcgc acgatgtctt gatcgagctc gtaatcgatg agctcgccgc
     1981 cgacggccag ctgcgcagca ccaccgcgca tatcgaacag gtcgccgtcg aatttgaagt
     2041 acaggttctt gatgacctga tcgcccacct ggatttgccc cgaatccatc agtgccgcgc
     2101 gcacggcgtc cgacgtgccg ttggcgaatg gatcgagcgc atttgccgcg gtcaacggcg
     2161 tgttcaacac gaccgtggtc gtgccgggga tcgacgccgc cgtggtgctg ccgccgccgt
     2221 tcgtcgtgcc gttgattgca aggttgaagc aagagccgca cagctgcccg aagttctgtt
     2281 gtcgcgacga gtcgagcccg atgaggaagc ccgcgttgaa agaccaggtg tcggtcagct
     2341 cgtagtcggc gtcgaaacgc ccgtagaccg tgtcgacgtc gctctcgatg tgcgcaccgg
     2401 ggccgagcaa agcgtcgccg ctgaagttga cggcaatcga cgacgtgccg gccgcaacgc
     2461 ccgtcggcaa ccggaagaac gggttgatct gcgccgcgtt cgcggcaccg gggccgaaca
     2521 tcgtcgcgct cgcattgccg cgcggcacgg cttggtcgtt ctcgcgctgc gagtagatca
     2581 agtcgcccgt caacgtcagc cgcgcggtta cgtcttgctc aatcgtcgcg aagacgctgt
     2641 gccggcgttc gctcgcaagc agatcggcgt gcagcgaggg gtcgcagtcg ccggcgatgg
     2701 ctccagccgc tgtgtacggc gtgtagtagg tctgccctgc caccgtgatg ctcgcggggc
     2761 cgcagcgatt cggcgtgaaa tcgcggccgc cgcgcgccgt ctgattggcc cgcacgtagt
     2821 cgcgatccac ggcatcgagg ttgtcgcgat ccgagtagtt gtaggacacg agcagcgacc
     2881 cgccgctcca agtcgtcccg ccgagcacgc ccgcgttgac cgtgccgtag ccgtcggcgg
     2941 cacccgtttg cacggacgcc tcgatgccgt cgacgttgcg ccgcgtgatg aagttgacga
     3001 cgccggcaac ggcgtcggaa ccgtagaccg acgaggcacc gtcggcgagc acctcgacac
     3061 gctcgagcgc aagaggcgcg actatgttgg gatccgcgag cacgtggttg attcccgaca
     3121 ccggcatgcg gtgcccattc agcaagatca acgtgctgtt cgacgccgag gccccgaggc
     3181 cgtggatcgt tggcgcgttg gtgccggcgc cgtcgaagct gccgtaggcc gcttgcccgg
     3241 cgctttgtag gccgacgacg gacgggacgg tcttcaagat ctgttgcacg gtttgcgcgc
     3301 cggtgttttc aatttcgccg cgcccgaccg tgacgagatt cgagccgacg ggtgccacgc
     3361 cgcgcaggct cgagccagta acgacgatct cctcgacatt cggcgcttgg cccgcggcat
     3421 tctgcgcgcc cgccgtagcg ctgactccca aagccgcaat gatcgatgaa cgaagcgtaa
     3481 gaccaacggt cgagctcatt tttgattccc cccaaaaagg ctccggagtt gcaaacgagg
     3541 cgttgtgtac gaactggtta tttacatgta ccagttggta catgtcaacg aaaattaggg
     3601 aacgagcaga cgaccgcacg cttccgctag ccggccgcga tctcgacgcg gcggacgtcg
     3661 ccgacgatga acacgtaggc agcggctcca atgaggccga gagccgcgat gtaggccaac
     3721 gcgccgtaaa agctgcccgt agcaccgagc acgaagccga tcacgagcgg cgtcgcaatc
     3781 ccggccagat ttgcgcagaa gttgaagacg ccgcccgtca agcccatgag gtgcttcggc
     3841 gccacgtcgg cgatcagcgt ccagccgaga ttcaccatgc cttggccgaa gaacgccacc
     3901 gacatgatcg cgatgacgag cgagttgctc ggcgtgaaat tggccaacac catcgtcgac
     3961 gtcagcaaca ggccggtgat gatcggcagc ttgcggccga gcgtcgccga gccggtgcgg
     4021 cgaatgatgc cgtcggagat cgcgccgccg acgagcacgc cgacggaggc cgcaatgtac
     4081 ggcagcgagg ccgcgaaccc ggagcgaatc caatccatgc cgcgctccgt cgcgagatag
     4141 cttgggaacc acgtcagaaa gaacaccaac gtgctgttgc cgcagaactg accgatcgac
     4201 gctccgacta tctggcgctg tcgcagcaac tggcgcacgg ccacccagct caacgcgaac
     4261 ccgccggccc cgctcgcaag cccaccgccg gccgcgatgt agtcgagctc cgcctgattg
     4321 acgctcgcac tctcgtgcgg ctcccgatac agccagtacc acgcgaatgc gtagacgatg
     4381 ccgacgccgc cgaagatgaa gaacagcgcg cgccaaccga acagcgcgac gatgtacgtc
     4441 aagacgggaa tcagaaaacc gaggccgacg tactggccga ccgagtagac gccgttcgcc
     4501 ctcgcccgct cggcttgcgg gaaccaggtc gagagaatcc ggctgttggc gggaaagcac
     4561 ggcgcttccg caatgccgag agcgagccgc gccgagaaca atcccgcgac gctcgacatc
     4621 agcccgtgca tcaccgtcgc cagcgaccaa gaggcgagcg atagaccgta cgtcagccgc
     4681 gcgccgagct tgtcgagcac gagcccgcct ggaatttgcg cgagcgcgta cgtccacgag
     4741 aacgccgaga agatcatgcc catgacctcg ggcccgatcg cgagctcgcg caccagagac
     4801 ggctgcgcga cgcccatgac ggagcggtcg acgtaattca gcatcgtagc gagcgtgatc
     4861 agcgtgagga cgccgaagcg cgcacgcgtt cgcgtcacgc tcgcgtcccc gccgcgtgcc
     4921 gctgcaacag gtgctgcacg gccagctcgt agccatgcac gcccagcccg gaaatgatgc
     4981 ccgtgacaac ctgcgtcatg atcgacttcg agcgccactc cgcctcacgc cgatgaatgt
     5041 tcgagatatg cacctcgacc acggggcaat cgaccatctt gatcgcgtcg agaattggat
     5101 agcccgcgta cgtgaatgca gccgggttga acgcgatgcc gaccgcgccg agacgcgctt
     5161 cctggaacca atcgaccatg acgtgctcgg cgttcgtttg ccgaaactcg agctttaacc
     5221 cgcgcgcgtc cgtcagcgcg cgacagcgcg cctcgacctc ggccaacgtg tcacggccgt
     5281 agatctccgg ctcccgctgg ccgagcagat tcaaattggg accgttgaac acgtagaccg
     5341 tcgtgcgcat tgcgccctcc tatagcgaca gcaggctatg gcgtcaatcg cgcgggcgat
     5401 tccccggcgg cgccggcggg cgctccgctg cgggcgcttg caagaggtcc aaggacgcgt
     5461 agttcggcca gtcgacgacg gtgatctccg tgccgagcga cacgacacgc ggccgccccg
     5521 gctcgaacac gggccaatcg tgcactcgcg cggacctcgg cttgccgtcg cgcgcgaatg
     5581 agacgagtaa cgctgccatg tccttttcaa gctttacgtc gacctcttcc caattgcgcg
     5641 tctgacggaa taagttcaac gagtcgcgcg tgcgtaagaa gtacgggatc tcgccggaat
     5701 ggtacgcacc ggccgtcgcg ggatcgtgat cgatgaaggt gattcccggc gcgtacggct
     5761 gccggcgcgt gaagaagtac gcgtacacag gcgagcggct gaacttggct tgagtgcgcg
     5821 cccagttcgc catctgcgcg ccgacggacg agtcgcggcc gagatcggcc gcagcacgcg
     5881 cggcgctcga cgcgtccttg gcgggatacg cggcaagcac cgcgctcgcg gtgctcggaa
     5941 atacccgccg cacagcggtc tcgagctcgg caaccgacgt gacgcgcccg agggaacgga
     6001 acgactcgtc tcgagtaaag ccgagcatga tcggcacgtc gctctgctgc ttcgattcga
     6061 acgccttggc ggcaccgatg acgtaatgac cgtcgagaac gatcggctcc cgggcgccgg
     6121 gcgcagtcgc gatgcgatcg ccgccgacgt cgcgcagctg ctcgatcgac tgtgcgccga
     6181 ccgctcgttg cagctccagg cccgcagctt cgccttgctc gagcgtcaca gccgtcagcg
     6241 gctcgctgaa cgggctgccg ctcattccaa cggctttgtg aaacaggccg cgtgccggcg
     6301 ggctcgcttg cagcagc
//
LOCUS       DATLEE010000552         6356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_645421, whole genome shotgun sequence.
ACCESSION   DATLEE010000552 DATLEE010000000
VERSION     DATLEE010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645421"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VM692_11910"
     CDS             complement(<1..561)
                     /locus_tag="VM692_11910"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HUQ52920.1"
                     /translation="MWLKPQSSFKDLREFLAHLEARGDLKTIEAPVSPALELTEISRR
                     VLAKRGPALLFARASPRGIRVLSNLFGTEERVAAAIGHDSREALTELGRLLAALRAPQ
                     PPRSVGEAWRSLPLLKEILNMAPKTVRNPPCQEVVVEGEAVDLADWPIQTCWPDDVAP
                     LITWGLTTTRGPAHERQNLGIYRQQVV"
     gene            655..1479
                     /gene="dapF"
                     /locus_tag="VM692_11915"
     CDS             655..1479
                     /gene="dapF"
                     /locus_tag="VM692_11915"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019627700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HUQ52921.1"
                     /translation="MKLAFTKMNGAGNDVMVVPWPSKHVPPSADVVRRWGDRRRGVGF
                     DQLLLVDSEHHTDGADATYRIFNADGSEVEQCGNGVRCLASFLAPQLGTELKLVSPAG
                     VIAARVESPARVSVDLGVPNFKPAELPFVTPAERERYRLDLAGRSVEFGAVSMGNPHA
                     VIAVDSVAAAPVEELGPALQRHASFPRSVNVGFMERLDSQRIRLRVYERGVGETLACG
                     TGAAAAVAVGRRWGVLGEKVAVELPGGTLEVNWPGPGAPLWQTGPTAAVYEGYIEL"
     gene            1476..2186
                     /locus_tag="VM692_11920"
     CDS             1476..2186
                     /locus_tag="VM692_11920"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HUQ52922.1"
                     /translation="MSQREIVAAVGAPLTDTVSEDSIADYLKSHPDFFERHAALMLGL
                     KLPHRAGGASISLVERQVSMLRQRNAQLERQLKDLVAVAKQNDVLVEKIHQLCLKLMR
                     APTLTARLEQLETGLREDFAAERAVLVLFPDAPAGGVREGFVRRLDPDDADVRPFATF
                     LRAAKPRCGPLRDRQKNIFDRDADSVSSAALVPLGPESKLGFLIIGNHDPDHFHPGKR
                     MDFLARLGEVLAVAIRNA"
     gene            2200..3099
                     /gene="xerC"
                     /locus_tag="VM692_11925"
     CDS             2200..3099
                     /gene="xerC"
                     /locus_tag="VM692_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HUQ52923.1"
                     /translation="MGRDELAAFLKRLATERRLSPHTVEAYERDLHALLDFCARGQIA
                     TFAALDSFNVRRFAAESHRRGLGARSVARRLSAVRTFLNYLIETGVLKSNAGVHVQAP
                     KAPKRLPATLDADQVASLLAISGDDPVTLRDRAILELFYSSGLRLAELVGLNLGDVDA
                     ADRTVRVLGKGSKARVVPVGKQALAALNDWLAVRSGLARAGELAIFVGRRGERVSPRT
                     VQKRVNEWAKRQGAPTGVHPHMLRHSFATHVLESSRDLRAVQELLGHASLSTTQIYTH
                     LDFQHLASVYDQAHPRARKRPSE"
     gene            3141..3683
                     /gene="hslV"
                     /locus_tag="VM692_11930"
     CDS             3141..3683
                     /gene="hslV"
                     /locus_tag="VM692_11930"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HUQ52924.1"
                     /translation="MSAPHFDVHGTTIVCVRRNGRVAIGGDGQVTLGNQVMKGNARKV
                     RRLHEGKVLAGFAGGAADAFTLFEYFETKLQKHGNLMRAAIEMAKDWRTDRMLRRLEA
                     LLIVADSQNSLLLSGTGDVIEPEHDMLAIGSGGAYAQAAAQALYENTELEARAIVEKA
                     LTIAGKICIYTNTSITVEEL"
     gene            3688..4992
                     /gene="hslU"
                     /locus_tag="VM692_11935"
     CDS             3688..4992
                     /gene="hslU"
                     /locus_tag="VM692_11935"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HUQ52925.1"
                     /translation="MSMTPREIVEELDKHIVGQHDAKRAVAIALRNRWRRAQIKEPLR
                     SEITPKNILMIGPTGVGKTEIARRLARLAHAPFIKVEATKFTEVGYVGRDVDSIVRDL
                     VDMAVKMTREEAVTRVADRAADAAEDRLLDSLLPGSRDDDGRDTRQRFRKMLREGDLD
                     DREVELDVAAGMPGVEIMAPPGMEEMTNQLQSLFQNLHQGRSKRRKLKVKDALGLLKE
                     EEAGKLINQEDVKLQAVKAVEENGIVFIDEIDKIARRAEAHGADVSREGVQRDLLPLV
                     EGCTVSTRYGMVRTDHVLFIASGAFSVAKPSDLIPELQGRFPIRVELTALGVDEFVRI
                     LTEPSASLTEQYKSLLATERFDIQFEPSGVRRLAEVAFQVNDRSENIGARRLHTVLER
                     LLESVSFDAADKGGQSVVIDAAYVDAHLQALVQDQDLSRYIL"
     gene            4989..5375
                     /locus_tag="VM692_11940"
     CDS             4989..5375
                     /locus_tag="VM692_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HUQ52926.1"
                     /translation="MSELQTPPPTDLKFHRRSRELEVRFADGLNARLSAELLRVHSPS
                     AEVKGHAAGEGMLVTGKEGVNIASIEPVGRYAVRIVFDDGHNTGLYTWAVLYELCAER
                     DRKWARYLERLAQAGQPRHPEPKWAE"
     gene            5448..6215
                     /gene="ubiE"
                     /locus_tag="VM692_11945"
     CDS             5448..6215
                     /gene="ubiE"
                     /locus_tag="VM692_11945"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HUQ52927.1"
                     /translation="MTAADPDSNDRVADFGYEQVPWGEKKARVRGVFDSVASRYDLMN
                     DVMSGGLHRLWKRFTIAKTGLRPGQQALDVAAGSGDLAVGLAGKVGRTGRVVVTDINA
                     AMLDEGRTRLLNAGLAGNIDYVLADAEALPFPQHSFHCITIGFGLRNVTDKDRALASM
                     YGALKPGGRLLVLEFSHAELGPLAPLYELYSFQLLPRFGEWLAGDSASYRYLAESIRR
                     HPDQETLKGMMERAGFERCEYFNLTAGIVALHVGYRL"
BASE COUNT         1050 a         2161 c         2141 g         1004 t
ORIGIN      
        1 gacgacctgc tgccgataga tcccgaggtt ctgccgctcg tgcgcgggcc cgcgggtcgt
       61 cgtgaggccc caggtaatca gcggcgcgac gtcgtcgggc cagcaggttt ggatcggcca
      121 gtcggcgagg tcgacggcct cgccctcgac gacgacttcc tggcacggcg ggttgcgcac
      181 ggtcttcggc gccatgttaa ggatttcttt cagcagcggc agcgagcgcc acgcttcacc
      241 gacgctgcgc ggcggttgcg gcgcgcgcag cgccgccaac aaccgaccga gctccgtcag
      301 cgcctcgcgg ctgtcgtggc cgatggccgc cgcgacgcgc tcctcggtgc cgaacaagtt
      361 gctgagcacg cgaatgccgc gcgggctcgc tcgtgcgaac aagagcgccg ggccgcgctt
      421 cgcaagcacg cgccgcgaga tctcggtgag ctccagcgcg gggctcaccg gagcctcgat
      481 ggttttcaag tcgccgcgag cttccagatg cgcgaggaac tcgcgcagat ctttgaagga
      541 gctctgcggt ttcagccaga tggaatccac ggccacttgc ctcttggttt tttggcctaa
      601 gatacgtgct ttccgcggcg ctggcccggc cgattcacgt ctccgcgacc cccgatgaag
      661 ctcgctttca ccaagatgaa cggcgcaggc aacgacgtca tggtcgtgcc gtggccgagc
      721 aaacacgtgc cgccctcggc cgacgtcgtg cggcgttggg gcgatcggcg gcgcggcgtc
      781 ggcttcgatc aactgctgct ggtcgacagc gagcaccaca cggacggcgc cgacgcgacc
      841 tatcgcatct tcaacgcgga cgggagcgag gtcgagcagt gcggtaacgg cgtgcgctgc
      901 ctcgcaagct tcctggcacc gcagttggga acggagctca agctcgtcag ccccgccggc
      961 gtcatcgccg cgcgcgtcga gtcacccgcc cgcgtcagcg tcgacctcgg cgttccgaac
     1021 ttcaagccgg cggagttgcc gttcgtgacg ccggccgagc gcgagcgcta ccggctcgac
     1081 ctggccggcc gctcggtcga gttcggcgcg gtctcgatgg gcaatccgca cgccgtgatc
     1141 gccgtcgact ccgtggccgc ggcgcccgtc gaagagctcg gccccgcgtt gcagcggcac
     1201 gcaagctttc cgcgcagcgt gaacgtcggt ttcatggagc ggctcgactc gcagcgcatt
     1261 cggctgcgcg tctacgagcg cggcgtcggc gagacgctgg cttgcggcac gggggcggcg
     1321 gcggccgtcg ccgtaggccg tcgctggggc gtgctcggcg aaaaagttgc cgtcgagtta
     1381 cccggcggca ctctcgaagt gaattggcct ggacccgggg cgccgctgtg gcaaactggg
     1441 cccacggcag cggtgtacga gggctacatc gaactatgag tcagcgtgag atcgtggcag
     1501 cagtcggcgc tcctttgacc gacaccgttt cggaagactc gatcgccgac tatctcaaaa
     1561 gccaccccga cttcttcgag cgccatgccg cactgatgct cgggctcaag ctcccgcatc
     1621 gcgccggcgg cgcctcgatc tcgctcgtcg agcggcaggt ctcgatgctg cggcagcgca
     1681 atgcgcaact cgagcgccag ctcaaggatc tcgtcgccgt cgcgaagcaa aacgacgtgc
     1741 tcgtcgagaa gattcaccag ctgtgcctga aactcatgcg cgcgccgacg ttgacggctc
     1801 ggctcgagca gctcgagaca ggtctgcgcg aggacttcgc cgcggagcgc gccgtgctcg
     1861 tgctgtttcc cgatgcgccg gccggtgggg tgcgagaagg gttcgtgcgc cgcttggacc
     1921 ccgacgacgc cgatgtccgg ccgttcgcga cgttcctgcg cgcggccaag ccacgctgcg
     1981 gcccgttgcg cgaccggcaa aagaacattt tcgatcgcga cgccgactcg gtcagctcgg
     2041 cggcgctcgt gccgctgggg cccgagtcga agctcggctt cctgatcatc ggcaaccacg
     2101 atcccgacca ctttcacccg ggtaagcgta tggacttctt agcgcgcctc ggcgaagtcc
     2161 tcgcggtcgc gattcgtaac gcctgagcaa cgagtagtcg tgggtcgcga cgagctcgca
     2221 gcattcctga agcgattggc gacggagagg cgcctctcgc cgcacaccgt cgaggcttac
     2281 gagcgcgatt tgcacgcgtt gctcgacttc tgtgcgcgcg ggcagatcgc aacgttcgcc
     2341 gcgctggact cgttcaacgt ccgccgcttc gcggccgaga gtcatcgccg cggcttgggc
     2401 gcgcgcagcg tggcgcgacg cctgtcggcc gtacgcacgt tcttgaacta cctcatcgag
     2461 accggtgtgc tgaagagcaa tgccggtgtg cacgtgcagg caccgaaagc cccgaagcgt
     2521 ttgcccgcga cgctcgacgc cgaccaggtc gcgagcctgc tcgcgatcag cggcgacgat
     2581 cccgtcacgc tgcgcgatcg ggcgattctc gagctgttct actcgtcggg cctgcggctc
     2641 gcggagctcg tcggcttgaa cctcggtgac gtcgatgcag ccgaccgcac cgtgcgcgtg
     2701 ctcggcaaag ggtccaaggc ccgcgtcgta ccggtcggca agcaggcgct cgcggcactg
     2761 aacgactggc tcgcggtgcg ctcaggccta gcgcgcgccg gcgagctcgc gatcttcgtc
     2821 ggccgccgcg gcgagcgcgt gagcccgcgg acggtgcaga agcgcgtcaa cgagtgggcg
     2881 aagcggcagg gcgctccgac cggcgtccac ccgcacatgc tgcggcattc gttcgcgact
     2941 cacgtgctcg agtcgagccg cgacctgcgc gccgtgcaag agctgctcgg ccacgcgagc
     3001 ctctcgacga cgcagatcta cacgcacctc gacttccagc acctcgcgag cgtctacgac
     3061 caggcccacc cgcgcgcgcg caagcgcccg tccgagtagc ccgagcggcg ctccgcgcgc
     3121 cgctggctta aaatccccgc atgagcgccc cgcatttcga cgttcacggc acgactatcg
     3181 tttgcgtgcg ccgcaacggc cgcgtcgcca tcggcggcga cggccaagtc acgctcggca
     3241 accaggtcat gaaaggcaac gcgcgcaagg tgcggcgttt gcacgaaggc aaggtgctcg
     3301 ccggcttcgc gggcggcgcg gccgacgcct tcacgctgtt cgagtacttc gagacgaagc
     3361 tgcagaagca cggcaacctc atgcgcgccg cgatcgagat ggccaaggac tggcgcacgg
     3421 accgcatgct gcgccggctc gaggcgctgc tgatcgtcgc cgactcgcaa aactcgctgt
     3481 tgctttcggg caccggcgac gtcatcgagc ccgagcacga catgctcgcg atcggctccg
     3541 gcggcgccta cgcgcaagcc gcggcgcagg cgctctacga gaacaccgag ctcgaggcgc
     3601 gcgcgatcgt cgagaaggcg ctgacgatcg cgggcaagat ctgcatctac accaatactt
     3661 cgatcacggt cgaagagctc tagcgccatg tcgatgacac cgcgcgaaat cgtcgaggag
     3721 ctcgacaaac acatcgtcgg tcagcacgac gcgaagcgcg ccgtggcgat cgctctgcgc
     3781 aaccgctggc ggcgcgcgca gatcaaggag ccgctgcgca gcgagatcac gccgaagaat
     3841 atcttgatga tcggccctac gggcgtgggt aagaccgaga tcgcacggcg gctcgcgagg
     3901 cttgcacacg cgccgttcat caaggtcgag gcgacgaagt tcaccgaggt cggctatgtc
     3961 ggccgcgacg tcgattcgat cgtccgcgac ctcgtcgaca tggccgtcaa gatgacgcgc
     4021 gaggaggccg tcacgcgggt cgcggatcgc gccgccgacg cggccgagga ccgcttgctc
     4081 gattcgctgc tgcctggctc acgcgacgac gacggccgcg acacgcggca acggttccgc
     4141 aagatgctgc gcgaaggcga cctcgacgac cgcgaggtcg agctcgatgt cgcggcgggg
     4201 atgcccggcg tcgagatcat ggcgccgccc ggcatggagg agatgacgaa tcagctccag
     4261 agcctatttc agaatctgca ccagggccgc tcgaagcgcc gcaagctcaa ggtcaaggac
     4321 gcgctgggcc tgctgaagga agaggaggcc ggcaagctca tcaaccaaga agacgtgaag
     4381 ctgcaggccg tcaaggccgt cgaagagaac ggcatcgtgt tcatcgacga gatcgacaag
     4441 atcgctcggc gcgccgaggc tcatggcgcc gatgtgtcgc gcgaaggcgt gcagcgcgat
     4501 ttgctgccgc tcgtcgaagg ctgcacggtg agcacgcgct acggaatggt gcgcacggat
     4561 cacgtgctgt tcatcgcgtc gggcgcgttc agcgtcgcca agccgtcgga cctgatcccg
     4621 gagctgcaag gccgcttccc gatccgcgtc gagctgacgg cgctcggcgt cgatgagttc
     4681 gtgcgcatcc tcacggagcc gagcgcgtcg ctgacggagc aatacaagag cctgctcgcg
     4741 accgaacgct tcgacattca gttcgagccg agcggcgtgc gtcggctcgc cgaggtcgcg
     4801 ttccaagtca acgatcgcag cgagaacatc ggcgcgcggc gcctgcacac cgtcctcgag
     4861 cgcttgctcg aaagcgtgtc gttcgacgcg gccgacaagg gcggccagag cgtcgtcatc
     4921 gatgccgcgt acgtcgacgc gcatttgcag gcgctcgtcc aagatcagga tttgagccgc
     4981 tacatcctat gagcgagctc cagaccccgc cgccgacgga cctcaagttc caccgcaggt
     5041 cgcgcgagct cgaggtccgg ttcgccgacg gcctgaacgc acggctatcc gccgagctgt
     5101 tgcgcgtgca ctcgccatcg gccgaggtca aaggccacgc ggccggcgaa ggcatgctcg
     5161 tgacgggcaa ggaaggcgtg aacatcgcga gcatcgagcc cgtcggccgc tacgccgtgc
     5221 gaatcgtttt cgacgacggc cacaacacgg ggctctacac ctgggccgtg ctctacgagc
     5281 tctgcgccga gcgcgatcgc aaatgggcgc gctatctgga gcggctcgca caagccggcc
     5341 aaccgcggca ccccgagccg aagtgggcgg aatgagcgtc acgtcggcgg cgtaataccg
     5401 agctcgtccc cccgccgcgt tccgcgggtg gcctatactt cacggccatg acggccgccg
     5461 atcccgactc gaacgaccgc gtcgccgact tcggctacga gcaggtcccg tggggcgaga
     5521 agaaggcccg cgtgcgcggc gtcttcgact ccgtcgcgtc gcgttacgac ctgatgaacg
     5581 acgtcatgtc gggcgggctg catcggctct ggaagcgctt cacgatcgcc aaaacggggc
     5641 tccgccccgg gcaacaggcg ctcgacgtcg ccgcgggcag cggcgatctt gccgtcgggc
     5701 tcgcgggcaa ggtcggccgc acgggccgtg tcgtggtcac cgacatcaac gccgcgatgc
     5761 tcgacgaagg ccgaacgcgg ctcttgaacg cgggcctggc cggcaacatc gactacgtgc
     5821 tcgccgacgc ggaggctctg ccgttcccgc agcacagctt tcactgcatc acgatcggct
     5881 tcggcttgcg caacgtcacg gacaaagacc gcgcgctcgc gagcatgtac ggcgcgctga
     5941 agcccggcgg ccgactgctc gtgctcgagt tctcgcacgc cgagctcggg cccctcgcgc
     6001 ctctgtacga gctgtattcg ttccaacttc tgccgcgttt cggcgagtgg cttgccgggg
     6061 attccgcgag ctatcgctat ctcgcggagt cgattcgccg ccatcccgat caggagacgc
     6121 tgaagggcat gatggagcgc gccggcttcg agcgctgcga gtacttcaac ctgacggccg
     6181 gcatcgtcgc gctgcacgtg ggttatcgcc tttgacgttc atcgagcgag agatcaatcg
     6241 ccacattggc gagtcgacgg ccgcgcgcga gctgctcgcg cggctcgcgg gtacgagctt
     6301 cgccgtgcac gtcgaaggcc tgagcctcaa cgtcgtgctg catgcggacg gcgagc
//
LOCUS       DATLEE010000553         5915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3316639, whole genome shotgun sequence.
ACCESSION   DATLEE010000553 DATLEE010000000
VERSION     DATLEE010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3316639"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VM692_11950"
     CDS             <1..285
                     /locus_tag="VM692_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52928.1"
                     /translation="YFVSATAFPGYLWQPTDAKLRQLAEVCVFLYVGEHDEYRWHDEM
                     RREADALSAGGTVARYTLERGQGHRLETLADAGAARLFDGFEEATRGCKR"
     gene            376..1971
                     /locus_tag="VM692_11955"
     CDS             376..1971
                     /locus_tag="VM692_11955"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HUQ52929.1"
                     /translation="MRRPLLAAAVAAVVQSSLLLSAANAQPFANAKASRAQYLGSTAR
                     ASGQCEQLNVRGIADAVEVSARNVAAEGNTPQHCRVSGVLSPEIAFEVALPAMWNGRL
                     YMIGNGGHAGDALDNPGRVAQVRQGLENGFAVAQTNTGHYASKEPGATFVLSNPQKAI
                     DYAYRAVHLTAVTAKEIANRYYAQPVAKAYWNSCSNGGRQGLLEAQRYPEDFDGIVAN
                     APWVDQTGFTIGAIWNQRAFAETHVSADKLALVGSRVMEVCDAVDGLRDGLIDDPRQC
                     KFNVARDVPSCSGSNAAGMCLTPAEAAAVQKVYDGPQSGGRQIFPGFMVGSEAVLPRP
                     NGPATSGWVGLIAPAEAGQRPADFGLAEATMRYLVLRPPQAEWDFMKFDFSRDPSSLA
                     RWGEQANATDTDLRDFRARGGKLLMTYGWADAILQPLMGVNYYEKAVAANGANGTDFL
                     RLFMIPGMAHCGGGLGPDQYDAVTAVVDWVEKGVAPDSMIASKIVNGTVTRSRPLCPY
                     PQVARYKGQGSIDEAATFECKAP"
     gene            1988..2914
                     /locus_tag="VM692_11960"
     CDS             1988..2914
                     /locus_tag="VM692_11960"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HUQ52930.1"
                     /translation="MTTTRTRRLVTWALVATVASAAALGQRDRSPQAKAEPGTAFTEC
                     RNCPAMVVVPAGRFTMGSPADEPERRDNEPQLEITFARPFALSRTAVTWDQWEACVRD
                     RWCDGAAVDIALRTREEDGQPNPDYKDWGRGARPVVGVSWFDAQNFVGWLNWKTRKDD
                     AYRLPSEAEWEYAARAGTTTAYPWGPKLDHNYGNFGIAGPGLGGKAEGRDVWTDTTAP
                     VASFPPNAFGLYDMHGNTFEWTEDCYEADRAHAPRDGSANKEGNCANRVFRNGTFMSN
                     PYMQRSARRGAPYPATRRGRNYLSFRVAMTID"
     gene            3022..4884
                     /locus_tag="VM692_11965"
     CDS             3022..4884
                     /locus_tag="VM692_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HUQ52931.1"
                     /translation="MRTSIATVSISGSLEGKLRAVAEAGFDGVEIFENDLLGFPGTPR
                     EVGRIIRELGMQCALFQPFRDLEGMPPEQLARAFQRMERKFDVMAELGTDLILLCSNC
                     SPLAVADRPRLVDDLAALGERAAKRGMRVGYEALAWGRHTYDHRDAWSLIRDVDHPSI
                     GLILDSFHSLARGIPSASIGDIRVEKLFFVQIADAPRLTMDYLSWSRHFRNMPGQGDL
                     PLAEFAEAIHRLGYEGFWSLEIFNDRFRAGSASGVAVDGYRSLQLLHDEVASRPRAPI
                     KSAMPPRVDVKGVEFIEFAASDEEAQALGSMLSALGFVPTAKHRRKAVTRWQQGAINI
                     VMNCEPEGLAHSFDAVHGASVCAIGLRVANVPAALVRAEQLAIPRFAQAVGPDELQIP
                     SVKGVGGSLIYFIEDGTEERVWQHEFTHPVVQAQAARSLNLERTDHIAQTMQYEEFLS
                     WLLYYVVLFDVAKTPQIEIADTLGLVQSQAVESRDRALRITLNGSLGAQTLSSRFLQH
                     YMGAGVQHLAFETGDIFAAAEAARANGLATLEIPHNYYDDIEAKFGLERELVERLAKL
                     HILYDRDGSAEYLQFYSRAFAKRVFFEIVERRGYEAYGAANASIRLAAQARFKA"
     gene            4976..5599
                     /locus_tag="VM692_11970"
     CDS             4976..5599
                     /locus_tag="VM692_11970"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUQ52932.1"
                     /translation="MRDRNSEAEQVRLNILDIAAQEFADKGLAGARVDEIADKINSSK
                     RMIYYYFGGKDELYRAVLEQAYAKIRTREAAANYEAMRAEEALKQYVGHTFDYHAEHP
                     EFVRLVMNENIHYGEHIGAVPGLKERNHSVIATLQAILDKGVKEGRFRQGLDPVELHM
                     LVSSLCFYNVSNRYTFAVNFGVDMASPRVARRRRELVIEAIARFCLK"
     gene            5679..>5915
                     /locus_tag="VM692_11975"
     CDS             5679..>5915
                     /locus_tag="VM692_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52933.1"
                     /translation="MSIRALVFALSIAAAQGVSLAEPRDFPVAAVASQNDVYPERVTH
                     FPGGVTSLADVTYSVLPGFRPLVLDLYLPSGDAPR"
BASE COUNT         1065 a         1909 c         1942 g          999 t
ORIGIN      
        1 tatttcgtct cggcgacggc tttccccggc tacctgtggc aaccgacgga cgccaagctg
       61 cggcagctcg ccgaggtgtg cgtgttcctc tacgtcggcg agcacgacga ataccgctgg
      121 cacgacgaga tgcggcgcga ggcggacgcc ttgagcgcag gcggcaccgt ggcgcgctac
      181 acgttggagc gcgggcaagg gcatcggctc gagacattgg cagatgccgg cgcggcgcgg
      241 ctgttcgacg gcttcgagga agcgacccgc ggctgcaagc gctagccgcc ggcgtcggcg
      301 gcgaattgac aggcgccctc gggcgcgcgt tcaatcgtcc cgttcccggg ggatcgccaa
      361 ccaaaggatc gtcagatgcg caggcctcta ctcgcggctg cggtggccgc cgtcgtccaa
      421 tcgagcttgt tgttgtcggc agcgaatgct cagccgttcg cgaatgccaa agcttcgcgg
      481 gcgcagtact tgggtagcac ggcgcgtgcg tcgggacagt gcgagcagct caacgtgcgc
      541 ggcattgccg acgccgtcga agtctccgcg cgcaacgtcg ccgcagaagg caacactccg
      601 cagcattgcc gcgtcagcgg cgtgctctca cccgagatcg cgttcgaagt cgctctgccg
      661 gcgatgtgga acgggcgtct gtacatgatc ggcaacggcg gtcacgccgg cgatgcgctc
      721 gacaatcccg gccgcgtggc gcaggtgcgg caagggctcg agaacggctt tgccgtggcg
      781 caaacgaaca cgggccacta cgcgagcaag gagccggggg cgacgttcgt tctgtcgaac
      841 ccgcagaagg ccatcgacta cgcgtatcgc gccgtgcatc tgacggccgt cactgccaaa
      901 gagatcgcga accgctacta cgcgcagccg gtcgccaaag cctactggaa ctcgtgctcg
      961 aacggcggcc ggcaaggcct gctcgaagcg cagcgctatc ccgaggactt cgacggcatc
     1021 gtcgccaacg ccccatgggt cgaccaaacc ggcttcacga tcggtgcgat ctggaaccaa
     1081 cgcgccttcg cggagacgca cgtctcggcc gataagctcg cgctcgtcgg cagccgcgtc
     1141 atggaagttt gcgatgccgt cgacggtttg cgcgacggtc tcatcgacga cccgcgccag
     1201 tgcaaattca atgttgcacg cgacgtgccg agctgctccg gtagcaatgc agcgggcatg
     1261 tgcctgacgc cggccgaggc ggctgccgtg cagaaggtct acgacggccc gcagagcggc
     1321 ggccgccaaa tcttcccggg cttcatggtc ggcagcgaag ccgtgctgcc gcggccgaac
     1381 ggccctgcga cgagcggctg ggtcggcttg atcgctccag ccgaggccgg tcaacgcccc
     1441 gcggatttcg gcctcgccga agcgacgatg cgctatctcg tgcttcgacc gccgcaggcc
     1501 gaatgggact tcatgaaatt cgatttcagc cgtgacccgt cgtcgctggc gcgctggggc
     1561 gagcaagcga atgccaccga taccgatttg cgcgacttcc gcgcgcgcgg cggcaagctg
     1621 ctgatgacgt acggctgggc cgacgcgatc ttgcagccgc tgatgggcgt caactattac
     1681 gagaaggccg tcgcggccaa cggcgccaac ggcacggatt ttctgcgcct cttcatgatt
     1741 cccggcatgg cgcactgcgg tggcgggctc ggccccgatc aatacgacgc tgtcacggcc
     1801 gtcgtcgatt gggtcgaaaa aggcgtcgcg ccggactcga tgatcgcgag caagatcgtc
     1861 aatggcaccg tcacgcgctc gcggccgctc tgcccgtatc cgcaggttgc gcgctacaaa
     1921 gggcagggca gcatcgacga ggccgcgacc ttcgagtgca aggctccgta acaggctcgg
     1981 caatcgaatg accacgacga gaacgcgccg gctcgtgacg tgggcgctcg ttgcgaccgt
     2041 ggcgagtgcc gcggcgctcg ggcaacgcga ccgcagtccg caagcgaagg cggagcccgg
     2101 cacggcgttc acggagtgcc gcaactgtcc tgccatggtc gtggtgcctg ccggccgctt
     2161 cacgatgggc tcgccggccg acgagcccga acggcgcgac aacgagccgc agctcgagat
     2221 cacgttcgcg cggccgttcg cgttgtcgcg cacggccgtg acgtgggatc agtgggaagc
     2281 ctgcgtgcgc gatcgatggt gcgacggcgc agccgtcgac atcgcgctgc ggactcgcga
     2341 ggaagacggc cagccgaatc ccgattacaa ggactggggc cgcggcgcgc ggcccgtcgt
     2401 cggcgtcagc tggttcgatg cgcagaactt cgtcggctgg ctaaattgga aaacccggaa
     2461 ggacgacgcg tatcggctgc cgtcggaagc cgaatgggag tacgcggccc gcgccggaac
     2521 gacgacggcg tatccatggg gaccgaagct cgatcacaac tacggcaact tcggcatcgc
     2581 gggcccaggc ttgggcggca aagccgaagg ccgcgacgtc tggaccgaca cgacggcgcc
     2641 cgtcgcgtcc tttccgccga acgcgttcgg cttatacgac atgcacggca ataccttcga
     2701 atggacggag gattgctacg aggccgaccg agcccacgct ccgcgggacg gctcggcgaa
     2761 caaagaaggc aactgcgcga accgcgtgtt tcgcaacggc accttcatga gcaacccata
     2821 catgcagcgc tcggcacggc gcggcgcgcc gtacccggcg acgcgccgcg gacgcaacta
     2881 cctgagcttc cgcgtcgcga tgacgatcga ttgatcttcg ctgggccgtg tcccggaggc
     2941 taacctcgtc gacacctcgc gcagcttttc acgagcgcgg ctcggttgcc ttcgtgtacg
     3001 aactagttca taacaatcga gatgagaacc tcgatcgcga ccgtctcgat cagcggctcg
     3061 ctcgaaggga agctgcgtgc tgtggccgag gccggcttcg acggcgtgga aatattcgaa
     3121 aacgatctgt tgggcttccc cggcacgcca cgtgaggtcg gccgcatcat ccgcgagctc
     3181 ggcatgcagt gcgcgctgtt tcagccgttt cgcgatctcg agggaatgcc gcccgagcag
     3241 ctcgcacgcg ccttccagcg catggagcgc aagttcgacg tcatggcgga gctcggcacg
     3301 gacttgatcc tgctgtgctc gaactgctcg ccgctcgccg tcgcggacag gccgcggctt
     3361 gtcgacgatc tggccgcgct cggcgaacgc gccgcgaaac gcggcatgcg ggtgggatat
     3421 gaggcgctcg catggggccg gcatacgtac gaccaccgcg acgcgtggag cttgattcgc
     3481 gacgtcgacc atccgtcgat cgggctgatc ctcgatagct ttcattcgtt ggcgcgcggc
     3541 atccccagcg cgagcattgg cgacattcga gtcgagaagt tgtttttcgt acagatcgcg
     3601 gacgcaccgc ggctgacgat ggactatttg agctggagcc ggcacttccg caacatgccg
     3661 gggcagggcg acttgccgct cgcagagttc gcggaagcga ttcatcggct gggctacgaa
     3721 gggttttggt cgctcgaaat tttcaacgac cggtttcgcg caggctccgc gagcggcgtt
     3781 gcagtcgacg gctatcgctc gttgcagtta ctgcacgacg aggtcgcatc gcggccgcgc
     3841 gcgccgatca agtcggcgat gccgccgcgc gtcgacgtca agggtgttga gttcatcgag
     3901 ttcgctgcca gtgacgagga ggcccaggcg ctcggtagca tgctttcggc gctcggcttc
     3961 gtgcctacgg ccaagcaccg ccgcaaggcc gtcactcgct ggcagcaagg cgcgatcaac
     4021 atcgtcatga actgtgagcc cgagggtctt gcgcatagct tcgacgccgt gcacggagca
     4081 tccgtgtgcg cgatcgggct acgcgttgcg aacgtgccgg ccgcgctcgt ccgtgcggag
     4141 cagctggcga tcccacgttt cgcgcaagca gtcggtcccg acgagcttca aatcccgtcg
     4201 gtcaaaggtg tcggcggcag tctcatctac ttcatcgagg acggcaccga agagcgcgtg
     4261 tggcagcacg agttcacgca cccggtcgtg caggcgcagg ccgcgcgctc gttgaacctc
     4321 gagcgtacgg accacatcgc gcagacgatg cagtacgagg aattcttgtc gtggcttctc
     4381 tactacgtag tgctgttcga cgtggcgaag acgccgcaga tcgagatagc cgacacactg
     4441 ggtctagtgc agagccaagc cgtcgagtcg cgagatcgcg cgctgcgaat caccttgaac
     4501 ggctcgctgg gcgcgcaaac gctctcgtcc cgcttcctgc agcattacat gggcgcagga
     4561 gtgcaacacc tggcgttcga gacgggcgat atcttcgcgg ccgccgaggc tgcgcgcgcg
     4621 aacggcttgg cgacgctcga gatcccgcac aattactacg acgacatcga agcgaagttc
     4681 gggctcgagc gtgagctcgt cgagcgtttg gccaagctcc acatcctcta cgaccgcgac
     4741 ggcagcgccg agtatttgca gttctacagt cgcgctttcg cgaagcgcgt gttcttcgag
     4801 atcgtcgagc ggcgcggcta cgaagcctac ggcgccgcca atgcctcgat ccgtctggca
     4861 gcgcaagccc gtttcaaggc ctaaagcgct ggattgcgcg tcgcaatcgc acgaaaatgg
     4921 cgccacccaa ccgctgccgg agtaatggcc cttggctcga gcaagaaaac cgcgcgtgcg
     4981 agatcgcaac agcgaagccg agcaggtgcg gctcaatatt ctcgacatag cagcacagga
     5041 attcgcagac aagggcctcg ccggggcgcg cgtcgatgag atcgccgaca agatcaattc
     5101 gtcgaagcgc atgatctact actacttcgg cggcaaggat gagctgtacc gcgccgtcct
     5161 cgagcaggct tacgcgaaga tccggacgcg cgaggccgct gccaactacg aggccatgcg
     5221 cgccgaagaa gcgctgaagc aatacgtcgg tcatacattc gactaccacg cagagcatcc
     5281 cgagttcgtg cggctcgtca tgaacgagaa catccactac ggcgagcaca taggcgccgt
     5341 gcccggcctc aaggaacgca atcacagcgt catcgccaca ctgcaggcaa ttctcgacaa
     5401 aggcgtgaag gaggggaggt ttcgccaagg cctcgacccc gtcgagctgc acatgctcgt
     5461 gagctcgctg tgtttctata acgtctcgaa ccgctacacg ttcgctgtga atttcggcgt
     5521 cgacatggcg tcgccaagag tcgcacgccg ccggcgggag cttgtcatcg aggcgatcgc
     5581 acgcttctgt ctcaagtaga gctcggccca tggcccgtcc gcagcgactt gcggtacagt
     5641 gtgtacgaaa tggtccatac acacggttgg agcttgccat gagcattcgc gccctcgtgt
     5701 ttgcactgtc gatcgctgcc gcccagggtg tctcgttggc ggagccgcgc gacttcccgg
     5761 ttgccgccgt cgcgagccag aacgacgtct atcccgagcg ggtcacgcat tttcccggcg
     5821 gcgtaaccag cctcgccgac gtcacttact ccgtgttgcc cggtttccgg ccactcgtgc
     5881 tcgatctgta cttgccgagc ggcgacgcgc cgcgg
//
LOCUS       DATLEE010000554         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5855914, whole genome shotgun sequence.
ACCESSION   DATLEE010000554 DATLEE010000000
VERSION     DATLEE010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5855914"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..761
                     /locus_tag="VM692_11980"
     CDS             279..761
                     /locus_tag="VM692_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52934.1"
                     /translation="MLKLVVAVLALVLASTASAARWKDLRVDASSEAAFERSLAAFKD
                     ELSAARRYVLGEALKDIWVKGTLEAEAAQREYTADDYYKQLHGLGYEQIVTLTDPSGD
                     TAKARHRTMLATLAPAPLPRRMPSVGPRPAGGWGSTPESLAQGMQQCHCGAPNGEQGN
                     "
     gene            complement(880..1704)
                     /gene="dapD"
                     /locus_tag="VM692_11985"
     CDS             complement(880..1704)
                     /gene="dapD"
                     /locus_tag="VM692_11985"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HUQ52935.1"
                     /translation="MSTLQPVIERAWERRTELGPTRADLEIREAVEECIAGLDSGELR
                     VAEQRDGRWVVNQWLKQAVLLSFRINDNRVVEAGHTRYFDKVPQKFTAFDTEQFRGGG
                     MRVVPGAVVRRGAYVAPDVVLMPSFVNIGAYVDKGTMVDTWATVGSCAQIGKRVHLSG
                     GVGIGGVLEPLQAAPTIIEDDCFIGARSEVVEGVVVERGSVLGMGVYIGQSTRVYDRE
                     TGEIHYGRVPAGSVVVAGNLPSKDGKYSLYCAVIVKKVDEKTRAKTSLNELLRTAD"
     gene            complement(1723..>2403)
                     /gene="glnD"
                     /locus_tag="VM692_11990"
     CDS             complement(1723..>2403)
                     /gene="glnD"
                     /locus_tag="VM692_11990"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the uridylylation or deuridylylation of
                     the PII nitrogen regulatory protein; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HUQ52936.1"
                     /translation="GFTEEYFLRHRPEEIAWHTRVLVERDSPGAITVDVNDVVVRGLA
                     AVAVYSPEETRSFMRATAVLDELGLTIVDARIVPLAKSSLDTYCVLESDGSTITERRR
                     LDEIRHRLLKALTAGSDQPLKVTRRAPRQVRMFSTPVQVALSRDGKNARTIVELVAGD
                     RPGLLFQIAKIFDQERVALLNAKILTVGERAEDVYFVTNADRQPLDDASAERLDKALR
                     EGLADSRN"
BASE COUNT          391 a          794 c          813 g          405 t
ORIGIN      
        1 catgtgccaa tcaaagtagg gcagcgtcgc ggaacgcgct tgccatccgg aagaaccgcc
       61 gcagtcgtct cggcccgctt gcagggccgc tcgagcgcgg gaacaacacc ctcgctcgca
      121 cgcgcttcgt gccaggctga cggcgctgag tctcgtcggc gcagaacggc cgcataggct
      181 ttacagccgt tcgtgtaggc gcggtgcggg aatgatcggc gagcgttatg ctgcgaggcg
      241 tgtcgctctt ttcgtggccg ccggctggag gtgctgtcat gttgaagctc gtcgttgccg
      301 tcttggcgct cgtgctcgct agcacggcca gcgccgcgcg ctggaaagat ttacgcgtcg
      361 acgccagcag cgaggccgcg ttcgagcgat cgttggccgc gttcaaggac gagctgtcgg
      421 cggcgcgtcg ctacgtgttg ggagaggcgc tcaaagacat ttgggtcaag ggcacgctcg
      481 aggccgaagc ggcgcagcgc gagtacacgg cggacgacta ctacaagcaa cttcatggcc
      541 tcggctacga gcaaatcgtc accttgaccg atccgagcgg tgacaccgcg aaggcccgtc
      601 accggacgat gttggcgact ctcgctccgg ccccgctccc gcgacggatg ccctcggtgg
      661 gtccgcggcc ggcgggcgga tggggctcga ctcccgagag tctcgcgcag ggcatgcagc
      721 agtgccactg cggtgccccg aacggcgagc aaggcaactg acgcttcgcc tcggggcgag
      781 tgcagcgacg agtgatctgc aacatgggcg gcccgggcgg tagcgagagg aatgaccgag
      841 cccgcgacgc agagccgcgg ccggtcgaca ggcacgagat cagtcggcgg tgcgcagcaa
      901 ctcgttcaac gacgtcttcg ctcgcgtctt ctcgtcgacc ttcttgacga tgaccgcgca
      961 atagagcgag tacttgccgt ccttcgacgg tagattgccg gcgacgacga cggagcccgc
     1021 gggcacgcgg ccgtagtgaa tctcgcccgt ctcgcggtcg tagacgcgcg tgctctggcc
     1081 gatgtagacg cccatgccga gcacgctgcc gcgctcgacg acgacgcctt cgacgacctc
     1141 cgagcgtgcg ccgatgaagc agtcgtcctc gatgatcgtc ggcgcggctt gcagcggctc
     1201 gagcactccg ccgatgccga cgccgcccga caagtgcacg cgcttgccga tctgcgcgca
     1261 gctgccgacg gtcgcccacg tgtcgaccat cgttcccttg tcgacgtaag cgccgatgtt
     1321 cacgaatgac ggcatcagca cgacgtcggg cgcgacgtag gcgccgcggc gcacgacggc
     1381 gccgggcacg acgcgcatgc cgccgccgcg aaattgctcg gtatcgaagg ccgtgaattt
     1441 ctgcggcacc ttatcgaagt aacgcgtgtg accggcctcg acgacgcggt tgtcgttgat
     1501 gcggaacgac agcagcacgg cttgcttcag ccactgattc acgacccagc gcccgtcgcg
     1561 ctgctccgca acacgcagct caccggagtc gaggcccgcg atgcattcct cgacggcttc
     1621 gcggatttcg aggtctgccc gagtcggtcc gagctcggtg cggcgctccc aggcgcgctc
     1681 gatgacgggt tgcagtgtgc tcaattgacg atcctcgagg gattagttgc gcgagtcggc
     1741 gaggccttcg cgcagcgcct tgtcgagccg ctcggcactc gcgtcgtcga gcggctggcg
     1801 gtcggcgttc gtcacgaaat agacgtcctc cgcgcgctca ccgacggtca aaatcttggc
     1861 gttcagcagc gctacgcgct cttgatcgaa gatcttcgcg atctggaaca gcaggccggg
     1921 ccggtccccc gcgacgagct cgacgatcgt gcgagcgttc ttgccgtcgc gcgacagcgc
     1981 gacctgcacg ggcgtcgaga acattcgcac ctggcgcggc gcacggcgcg ttaccttcaa
     2041 aggttgatcg ctaccggccg tcaatgcctt cagcaggcga tgtcggatct cgtcgagccg
     2101 gcggcgctcg gtgatcgtgc tgccgtcgct ctcgagcacg cagtaggtgt cgaggctcga
     2161 cttcgcgagc ggcacgatac gggcgtcgac gatcgtgagg ccgagctcgt cgagcacggc
     2221 cgtcgcgcgc atgaacgagc gtgtctcctc gggagagtag acggcgacgg ccgcgaggcc
     2281 ccgcaccacg acgtcgttga cgtccactgt gatcgcgccg ggactgtcgc gctcgacgag
     2341 cactcgcgtg tgccacgcga tctcctcggg cctatggcgc aagaagtact cttccgtgaa
     2401 ccc
//
LOCUS       DATLEE010000555         4297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_651817, whole genome shotgun sequence.
ACCESSION   DATLEE010000555 DATLEE010000000
VERSION     DATLEE010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651817"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VM692_11995"
     CDS             complement(<1..338)
                     /locus_tag="VM692_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUQ52937.1"
                     /translation="MRALLFALVAAIVALPWQLEAQTADEPSGAAVYAQRCAACHDQV
                     DARIPPRAALTEMSPARVLRTLDFGLMMSIAYPLTRGERAAVATFLGKGRDETTPLPS
                     ALCGPETRIMA"
     gene            complement(466..1980)
                     /locus_tag="VM692_12000"
     CDS             complement(466..1980)
                     /locus_tag="VM692_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUQ52938.1"
                     /translation="MTACVQPRSAWMDVAPRAFAPLERNSTADVCVVGAGIAGLSTAY
                     ALACEGRSVVVLDDGAIGGGMTQRTTAHLSNAVDDRYFEIERMHGEDGARVVAASHTA
                     AIDRIEQTAAGEGIDCDFERVDGFLFVPPDESHEILEREIAAAHRAGLTGVEWLELQP
                     LGAQFLEKCLRFPRQAQFHPLKYLNGVAEAVARRGGQIFCGTHAASVEGRKALRVETT
                     SGHKVSAGAVVVATNTPINDVVAIHTKQAPYSTYVVALRIPALGANHALYWDTLDPYH
                     YVRLQRYSDYELLIVGGEDHKSGQANDGEERFARLERWAREHFPIAEAVELRWSGQVM
                     EPADGVAFIGRNPGDSDGVYIATGDSGMGMTHGTIAGILLTDLIMGRASPWTSLYDPS
                     RKMVHGVVEFAKENVNVAAQYLKDYASGGERSSADELAPGEGAILRRGLAKIAAFRDE
                     DGTLHELSATCPHLGCVVSFDAVEKTWNCPCHGSRFDCHGHVVVGPANRDLQRA"
     gene            complement(2057..3196)
                     /locus_tag="VM692_12005"
     CDS             complement(2057..3196)
                     /locus_tag="VM692_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-independent formaldehyde
                     dehydrogenase"
                     /protein_id="HUQ52939.1"
                     /translation="MKAVVYNGPRDIVVKDMPDAQIAYPTDVLVRVTSTNICGSDLHM
                     YEGRTSLEKGRIIGHENLGEIVEVGAAVRRVEVGDRVCVPFNCSCGYCKNCERGLTSA
                     CLTMNPGHAGAGYGYAEMGPYQGGQAEYLVVPYGDFNCLRLPEDAEEKESDYVMLADI
                     WPTGYHAVELSGLQAGESIVIYGGGPVGLMAALSARIKGASQIMVVDRHPDRLKLAEE
                     IGAIGIDDSDGSPVEQVLELTDGEGADRGAECVGWQAHDHGGREHPEMVLNDLVASVR
                     PTGGLGIIGVYVPEDPKGPNKLAKEGKIPFDFGEFWTRGQSMGTGQAPVKRYNRYLMN
                     LIHAGRATPSWIVSHELPLDQAPSAYRHFDARDDGWTKVVLKPAA"
     gene            3372..3887
                     /locus_tag="VM692_12010"
     CDS             3372..3887
                     /locus_tag="VM692_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52940.1"
                     /translation="MRTQRVIAIGGSRPSREAYAPSDPLAGLLPAGNPRYLRDPWGAE
                     DLSGIGGSHRVLVLGAGLAAVGVVLELDDQGHHAPIRVLSSPALLPHSPRLAPAVAGR
                     LAALLAAGRLELFAGEVRGAAAYGDTFVVDVLPHGRALHSSERYDWIVNCSPASVARP
                     HAPTITEHRWS"
     gene            3937..>4297
                     /locus_tag="VM692_12015"
     CDS             3937..>4297
                     /locus_tag="VM692_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUQ52941.1"
                     /translation="MYSSPHAPLEFPQLPLADFVLARAGQRGKRAALICAVTGRTIAY
                     EELPGLVDGTAAGLGALGIRKGDVCAVFAPNSPDYVIAMLALARLGAVTTTASPAYTK
                     DDLKKQLEDSRARVLLTS"
BASE COUNT          665 a         1445 c         1448 g          739 t
ORIGIN      
        1 gccatgatgc gcgtctcggg cccacatagc gcgctcggca gcggcgtcgt ctcgtcacgc
       61 cccttgccca agaacgttgc gacggctgcg cgctcgccgc gcgtcagggg atacgcgatg
      121 ctcatcatca agccgaagtc gagcgtgcgc agcacgcgcg ccggcgacat ctcggtcaac
      181 gccgcacgcg gcggaatgcg cgcgtcgact tggtcgtggc atgcggcgca gcgctgcgcg
      241 tagaccgctg cgcccgacgg ctcgtcggca gtctgcgcct cgagttgcca cggcagcgcc
      301 acgattgcag cgacgagcgc gaagagtagg gcgcgcatag cgggaagctc ctggtacgcg
      361 aaggctcctg tcgagagtcg caacgactag agcacggcgc tggaataggg gtcaaggcgg
      421 gccggcaccc cgctcgtggg ggcgacgcgg cgtcggcgcg gccgatcagg cgcgttgcaa
      481 gtcgcgattg gcagggccga cgacgacgtg cccgtggcaa tcgaaccgag agccgtggca
      541 cgggcagttc cacgtcttct cgacggcgtc gaagctcacg acgcaaccga gatgcgggca
      601 ggtggccgag agctcgtgca gcgtgccgtc ctcgtcgcgg aacgccgcga tcttcgcgag
      661 gccgcggcgc aggatcgcgc cttctccggg tgcgagctcg tcggccgagc tgcgttcgcc
      721 gccgctcgcg tagtccttga ggtactgcgc cgcgacgttg acgttctcct tcgcgaactc
      781 gacaacgccg tgcaccattt tccgcgacgg gtcgtagagc gacgtccacg gggacgcgcg
      841 gcccattatc agatcggtca gaagaattcc ggcgatcgtg ccgtgcgtca tgcccatgcc
      901 ggaatcgccc gtcgcgatgt acacgccgtc ggagtcgccg gggttgcggc cgatgaacgc
      961 cacgccgtcg gccggctcca tgacctggcc ggaccagcgc agctcgaccg cctctgcaat
     1021 cggaaagtgc tcgcgcgccc agcgttcgag acgcgcaaaa cgttcttcac cgtcgtttgc
     1081 ctgtcccgac ttgtgatcct cgccgccgac gatgagcagc tcgtagtcgg agtaacgctg
     1141 caatcgcacg tagtgatacg gatcgagcgt gtcccagtac agcgcatggt tggcgccgag
     1201 cgcggggatg cgcagcgcca cgacgtaggt gctgtaaggc gcttgcttgg tatgaatcgc
     1261 cacgacgtcg ttgatcggcg tgttggtcgc gacgacgacg gcgccggcgc tgaccttatg
     1321 gccgctcgtc gtctcgacgc gcagtgcctt gcggccctcg acgctggccg cgtgtgtgcc
     1381 gcagaagatt tggccgccgc gtcgtgctac ggcttcagcg accccgttca agtacttcaa
     1441 gggatgaaac tgcgcttggc gcggaaagcg caagcacttc tcgagaaact gcgcgccgag
     1501 cggttgcagc tcgagccatt cgacgccggt gagcccggcg cggtgcgcgg ccgcgatctc
     1561 acgctcgagt atctcgtgcg actcgtccgg cgggacgaac aggaaaccgt cgacgcgctc
     1621 gaagtcgcaa tcgatgcctt cacccgcggc cgtctgctcg atgcggtcga tcgcggccgt
     1681 gtgacttgcg gcgacgacgc gcgcgccgtc ttcgccgtgc atccgctcga tctcgaagta
     1741 acgatcgtcg acagcgttcg acaagtgagc cgtcgtgcgc tgcgtcatcc cgccgccgat
     1801 cgccccatcg tcgagcacga ccacggaccg gccttcgcag gcgagcgcat aagccgtcga
     1861 cagacccgcg atccctgcgc cgacgacgca cacgtcggcc gtggaattgc gttcgagcgg
     1921 cgcaaacgcg cgcggggcga cgtccatcca agcagagcgg ggctgcacgc aagccgtcat
     1981 ggtggcctcc tttcgcgcgt ggccgcgcaa tcagggcccc gcgctgcacg tttcgtgccc
     2041 cgaagccgcc gcattctcag gcagccggct tcagcacgac cttggtccac ccgtcgtcgc
     2101 gagcgtcgaa gtgccggtaa gcgctcggcg cttggtcgag cggcagctcg tgcgacacga
     2161 tccacgacgg cgtcgctcgg cccgcgtgga tcagattcat caggtagcgg ttgtagcgct
     2221 tcacgggcgc ctggcccgtg cccatgctct ggccgcgcgt ccagaactcg ccgaagtcga
     2281 atggaatctt gccttccttc gcgagcttgt tcgggccttt cgggtcctcg ggcacgtaga
     2341 cgccgatgat gccgagaccg cccgtcggcc gaaccgacgc gacgagatcg ttcagcacca
     2401 tctccgggtg ttcccttccg ccgtgatcgt gcgcctgcca gccgacgcat tcggcgccgc
     2461 gatcggcgcc ttcgccgtcc gtgagctcga gcacttgctc gacgggcgag ccgtcgctgt
     2521 cgtcgatgcc gattgcgccg atttcttcgg cgagcttcag ccgatccggg tgccggtcga
     2581 cgaccatgat ctgcgacgcg cctttgatgc gtgccgacag cgcggccatc aggcctacgg
     2641 gtccgccgcc gtaaattacg atcgactcgc cggcttgcag acccgagagc tcgacggcgt
     2701 ggtagcccgt gggccagatg tcggccagca tcacgtagtc ggactctttc tcctcggcgt
     2761 cctcgggcag ccgcaggcaa ttgaagtcgc cgtacggcac gacgaggtat tcggcttggc
     2821 cgccttgata cgggcccatc tcggcgtagc cgtagccggc gccggcgtgg ccggggttca
     2881 tcgtgagaca cgcgctcgtg aggccgcgct cgcagttttt gcaatagccg cagctgcaat
     2941 tgaacggtac acacacgcgg tcgccaactt cgacgcggcg cacggctgcg ccgacctcga
     3001 cgatttcgcc gaggttctcg tggccgatga tgcggccttt ttcgaggctc gtgcgccctt
     3061 cgtacatgtg cagatcggag ccgcaaatgt tagtgctcgt gacccgcacg agaacgtcgg
     3121 tcggataggc gatctgcgcg tcgggcatgt ccttgacgac gatgtcgcgg ggtccgttgt
     3181 agacgacggc tttcatgggc tctcctttcc ttgaatgcgg ctgccgaagg ggcgaaggtt
     3241 ccagcgtgca agatcgatgc ccgcgcagcc gccgcgaagt aaccgaagta agacctcgat
     3301 gagactgacg tgcgactcgc gggccgctag tttgacggcg tcgcaaaagc attttctcga
     3361 ggagcgaaag gatgcgcaca caacgagtaa tcgccattgg cggttcgcga ccgagtcgcg
     3421 aagcgtacgc accgtcggat ccgttggccg ggctgctgcc ggccggcaat ccgcgttatc
     3481 tgagggaccc gtggggagcc gaggatcttt ccggcatcgg cggttcccac cgcgtgctcg
     3541 tgctcggcgc ggggcttgcg gcggtcggcg tcgtgctcga gctcgacgat caaggccatc
     3601 acgcaccgat ccgggtcctc tcgagcccgg cattgctacc gcactcgccg cggctcgcgc
     3661 ctgccgtcgc cgggcggctc gcggcgctgc tcgcggccgg ccggctcgag ctgttcgccg
     3721 gcgaggtgcg aggggcggct gcctacggcg acaccttcgt cgtcgacgtt ctgccgcacg
     3781 ggcgagcgct gcattcgagc gagcgttacg actggatcgt gaactgctcg ccggcgagtg
     3841 tcgcgcgtcc gcacgcgccc acaatcacgg agcatcgctg gtcgtgacag ggtattctcg
     3901 cgtgcaccgc agggcacaga agccttccgg agcaccatgt actcaagccc acacgcgccg
     3961 ctcgagtttc cacagctacc gctcgccgat ttcgttctgg cgcgtgcagg ccagcgaggc
     4021 aagcgcgctg cgctgatttg cgcggtcacg ggccgcacga tcgcgtacga ggagctgccc
     4081 ggcctcgtcg acggcaccgc ggccgggctc ggcgcgctcg gcattcgcaa gggcgacgtt
     4141 tgcgccgtgt tcgcgccgaa ctcgcccgac tacgtgatcg cgatgctcgc gctggcgcgc
     4201 ttgggcgccg tcacgacgac ggcgagcccc gcgtacacga aggacgatct caaaaagcag
     4261 ctcgaggatt cacgcgcgcg cgtgctgctg acgtcgg
//
LOCUS       DATLEE010000556         3137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5121309, whole genome shotgun sequence.
ACCESSION   DATLEE010000556 DATLEE010000000
VERSION     DATLEE010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5121309"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VM692_12020"
     CDS             <1..364
                     /locus_tag="VM692_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUQ52942.1"
                     /translation="FGVVSLTLAAVGTYGLVSFSVNQRFNEIAVRSAFGAERGTLLKM
                     FMKAALRLAGIGVVVGVAGAIAMRQVVASQLYETSALDPWVLVLVPLTMLAVTALASY
                     LPARRASRIDVTQALREN"
     gene            complement(361..1308)
                     /locus_tag="VM692_12025"
     CDS             complement(361..1308)
                     /locus_tag="VM692_12025"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HUQ52943.1"
                     /translation="MSTRPHPSFLHFIRTVVAVAAALAVTVPGSRSIHAQAAADKIIV
                     SGASGNLGGLTVDELLARGVPANQLILVSRTPNELGKYKDMGASVRFGDFTKPESLPT
                     AYAGGTKMLLISVGGSGEVTAAELLTRAIDAAKAAGVKHIAYTSYVGITRGDTAGRAA
                     DHQATEEALRASGIAWTMLRNSIYMHGLIGQAARMVADGRAVIPPNESTIGYVTREDC
                     ARAAAAVLSTPGHENMAYDITGPELLGVRETAAVASAVTGKPIEVVQGGADVRPGFGR
                     PAMSFTTSHFKDLTGREPTSLRQLFAASKDALLTPPAAR"
     gene            1452..2873
                     /locus_tag="VM692_12030"
     CDS             1452..2873
                     /locus_tag="VM692_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52944.1"
                     /translation="MRGSLKKAAVFTAAALVASYAGAQQIANTGFKSVGRGWPLAADL
                     RDYELTGPAIPVVFENGAVSRGSWDKFIGSAHHGAAPKGVEPLPVDIYTSKDFYQDRA
                     LWTDQRYFRCNSPAGIEEAWGANTTATIGDNPPGSAAWGYCDRDYPREAIVSPYPFKT
                     AQEHYTALLAETTKRGGAKHPTAEQLEDWTGRYAHPGNTTDAQADWTGRNKSNNPQSY
                     WYRGRHSQIPTYLSLLTPEYQTRFVQEAYHAGNTNAPQWPSQYCWPEGFMRRWHEFAV
                     WEWHIIAAPSVVQIVTGVARNFITTVHVDREMKTDGSVPRLGQDVPRWYGETVGFWDG
                     DTLVTWTSNIQGWKVHSAFEYSNKMQTIEIYTPNRDPSGKFLGLNHEAIFYDPEALVE
                     PIRIIRNYVKTSGYDKGDPYVFIDCVQTIFPIEGRSTPVSPGTVIEYEVPDMYGRPWA
                     HYWEKYFEQGMKKPEGEDIFSFD"
     gene            2923..>3137
                     /locus_tag="VM692_12035"
     CDS             2923..>3137
                     /locus_tag="VM692_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52945.1"
                     /translation="MDIKRTTCGLVAATLTAATVVAVPVYAHHSFSIFDAEQTKTFTG
                     VVTRVNPDANHLQIFFAPMNDERKNVLR"
BASE COUNT          542 a         1013 c         1039 g          543 t
ORIGIN      
        1 gttcggcgtc gtgtcgctga ctcttgcggc cgtcggcacg tacggactcg tgtcgttctc
       61 ggtgaatcag cggttcaacg agatcgccgt gcgctcggcg ttcggcgccg agcgcggcac
      121 gctgctgaaa atgttcatga aggcagcgtt gcggctggca ggtatcggcg tcgtcgtcgg
      181 cgtagccggc gcgatcgcga tgcggcaagt cgtcgcgagc cagctctatg aaaccagcgc
      241 actcgatccg tgggtgctcg tgctggttcc gctgacgatg ctcgccgtga cggcgctcgc
      301 gtcgtacctg ccggcgcgcc gcgcgtcacg catcgacgtg acccaggcgc tgcgcgagaa
      361 ctagcgcgca gcgggcggcg tcagcagggc gtctttgctc gcggcgaaca gctggcgcag
      421 gctcgtcggc tcgcgacccg tcaggtcttt gaagtggctc gtcgtgaagc tcatcgccgg
      481 ccgcccgaag cccggccgca cgtcggcgcc gccttgcacg acctcgatcg gcttgcccgt
      541 gacggccgac gccacggccg ccgtctcgcg cacgccgagc agctcggggc ccgtgatgtc
      601 gtaggccatg ttctcgtgcc ccggcgtgct aagcaccgcc gccgccgcgc gcgcgcaatc
      661 ctcgcgcgtg acgtagccga tcgtgctctc gttcggcgga atcacggcgc ggccgtctgc
      721 gaccatgcgt gcggcttgcc cgatcaggcc gtgcatgtag atcgagttgc gcagcatcgt
      781 ccaggcgatg ccgctcgccc tcagtgcttc ctcggtcgct tggtgatcgg cggcgcggcc
      841 ggcggtgtcg ccgcgcgtga tcccgacgta cgacgtgtag gcgatgtgtt tgacgccggc
      901 ggccttggcc gcgtcgatcg cgcgcgtcag cagctccgcg gccgtcacct cgccgctgcc
      961 gccgacgctg atcaagagca tcttcgtgcc gccggcatac gcggtcggca gcgattccgg
     1021 tttcgtgaag tcgccgaacc ggacgctggc tcccatgtct ttgtacttgc cgagctcgtt
     1081 gggtgtgcgg ctcacgagga tcaattggtt cgcgggcacg ccgcgcgcga gcagctcgtc
     1141 gaccgtgagg ccgcccaagt tgccggaggc gcccgaaacg atgatcttgt cggcagcagc
     1201 ttgggcgtgg atcgagcggc tgccgggcac cgtcaccgct agcgcggcgg ccactgccac
     1261 gacggtgcgg atgaagtgca ggaacgaggg gtgcggacga gtgctcatga gcgctaaccg
     1321 tggtttccaa aggccgcggt actttaccga actgcccgcg cgagcggggc acgaacgggg
     1381 attcttaagt cgctgtaact cgcgcccgcg ggcctttcag ttaagctttg cattactgac
     1441 tgggggatta gatgcgcggc agcctcaaaa aggcggcggt tttcacggcg gcggccctag
     1501 tcgccagcta cgcgggcgcg cagcaaatcg ccaacacagg cttcaagagc gtcggtcgag
     1561 gctggccgct ggctgcggac ttgcgcgact acgagctcac gggcccggcc atccccgtcg
     1621 tcttcgagaa cggcgccgtg tcgcgcggct cgtgggacaa gttcatcggc tccgcgcacc
     1681 acggcgcggc gccgaagggc gtcgagccgt tgcccgtcga catctatacc tcgaaagatt
     1741 tttaccaaga ccgcgcgctc tggaccgacc agcgttactt ccgctgcaac agcccggccg
     1801 gcatcgaaga ggcatggggc gcgaacacga ccgcgacgat cggcgacaac ccgccgggct
     1861 cggctgcctg gggctattgc gaccgcgact atccgcgcga agcgatcgta agcccgtatc
     1921 cgttcaagac ggcgcaagag cattacacgg cgctgctcgc cgagacgacg aagcgcggcg
     1981 gcgcgaagca cccgacggcg gagcaactcg aggactggac gggccgttac gcgcatcccg
     2041 gcaatacgac ggacgcgcag gcggactgga cgggccgcaa caagagcaac aacccgcagt
     2101 cgtactggta tcgcgggcgg cacagccaga tcccgacgta cttgtcgctg ttgacgccgg
     2161 aatatcaaac gcgcttcgtg caagaggcct atcacgccgg caacacgaac gcgccgcagt
     2221 ggccgtcgca gtactgctgg cccgaaggct tcatgcgccg ctggcacgag ttcgcggtct
     2281 gggagtggca catcattgcg gcgccgtcgg tcgtgcagat cgtgacgggc gtcgcgcgca
     2341 acttcatcac gacggtccac gtcgatcgcg agatgaagac ggacggctcg gtgccgcgtt
     2401 tgggtcagga cgtgccgcgt tggtacggcg aaaccgtcgg cttctgggac ggcgacacgc
     2461 tggtcacgtg gacgtcgaac attcaaggct ggaaggtgca cagcgcgttc gagtactcga
     2521 acaagatgca gacgatcgag atctacacac cgaaccgcga cccgtccggt aagttcttgg
     2581 gtctcaatca cgaagcgatc ttctacgacc ccgaagccct cgtggagccg atccgcatca
     2641 tccgcaacta cgtcaagacg agcggctacg acaagggcga cccctacgtc ttcatcgatt
     2701 gtgtgcagac gatcttcccg atcgagggcc ggtcgacgcc ggtctcgccc gggaccgtaa
     2761 ttgaatacga agttcccgat atgtacggcc ggccctgggc gcattattgg gagaagtact
     2821 tcgagcaggg catgaagaag ccggaaggcg aggatatctt cagcttcgat taagctagct
     2881 tcctataaag tacccgctat tgagacagag gagtctcgga ttatggacat caagcgcaca
     2941 acctgcggcc tcgttgccgc caccctcacg gccgccacgg tggtcgccgt gcccgtgtat
     3001 gcgcatcact cattctcgat tttcgacgcg gagcagacga agaccttcac gggtgtcgtc
     3061 acgcgcgtga atcctgatgc gaaccacctg cagatcttct tcgcgccgat gaacgacgag
     3121 cgcaagaacg tgctgcg
//
LOCUS       DATLEE010000557         5938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7773200, whole genome shotgun sequence.
ACCESSION   DATLEE010000557 DATLEE010000000
VERSION     DATLEE010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7773200"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /gene="pabB"
                     /locus_tag="VM692_12040"
     CDS             complement(<1..671)
                     /gene="pabB"
                     /locus_tag="VM692_12040"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="para-aminobenzoate synthase component 1; catalyzes
                     the formation of 4-amino-4-deoxychorismate from chorismate
                     and L-glutamine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HUQ52946.1"
                     /translation="MSTPRIIELAHWDEVARRFRTLAALPWATWLDSAAGNAGDRFDI
                     LVADPYVTLRTRGATTEVTKRDGARSRSQRSPFKLVREQLGEVSSGAEGLPFAGGAVG
                     YFGYDLGRRLERIPSIAAADIAMPDLAIGLYDWAVVVDHAARRTWLVGNGRDARTFAQ
                     WPELIARLSAEPPPQPQAFRVLQPPRPNLDRGQYAAAFRAVQDHIRRGDCYQVNLTQR
                     FEAQA"
     gene            complement(735..1520)
                     /locus_tag="VM692_12045"
     CDS             complement(735..1520)
                     /locus_tag="VM692_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52947.1"
                     /translation="MLRRLLINALAASVLSGAAATADAAMHATQEFESGTTRPRTIAF
                     LPPQAMLIKKKVVQTEQQIEESGELANYLGASVLAAFKEQGYDVRVLTPNDVSADPEL
                     QELVLDASRRYNEMLTQLRLRLPRQIGKRRYEAGDEMRALAAKLGVDAVGFAEIQIYA
                     AAAGASAVSILTGFGSSGSTTLISVSVIDGATANIEAFFVPPVMRRGSIAGYDAIMEN
                     PAARIGELTEATLRDLPKVDAAARSASESDEDVLSDVEGLLEK"
     gene            complement(1498..2790)
                     /locus_tag="VM692_12050"
     CDS             complement(1498..2790)
                     /locus_tag="VM692_12050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HUQ52948.1"
                     /translation="MRALLTAAAALSAATALADGVYEPVPLKDQEQRLIDESRELHGY
                     FERQSLLYNDPRVVELVASVGRRLAPAVKDDYVQYRFFVLRDPSPNAFALPNGDIYIH
                     TGMLARLTDTAQLAAILGHEVNHVAGHHGILSFRSANKKIVASMVLTGVFGGLGSVIS
                     SGLAASMYGFSRELEQEADDHAVDLLLASPYDAKALPEIYGVLARDYEGITPRIPTIW
                     STHPQLEARAERTREQVAGAPDGRRDTEQFDAVVFPVRTMTIRDYVQDDYPRTAMALA
                     ENLAARYPEHPELVQLTGDAWAAMGALTQFDEEELSNRERARNANRRVMRTRQEREAQ
                     LLKTPEGQAALRENLGQARASYERALALDANFAPAYRGLGEVEERLGDPRAAAAAYVE
                     YLRKAPNASDRTVVVGRLRTLRDQLRTEETGNAATTSN"
     gene            2870..3640
                     /locus_tag="VM692_12055"
     CDS             2870..3640
                     /locus_tag="VM692_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ52949.1"
                     /translation="MTHFTRRTLRVNGIDTVVLEAGSGPPLVYLHGAGTVTGFDFAEA
                     WSRTFRVVIPYHPGFGLSADDPAVDEIHDYVLHYLELFDTLELRQFRLVGQSMGGFIA
                     TKFAIEHGERIEKLVLVCPIGLPVPPGMQTVNFLAVPPEELPALLAYDPQTVIKHLPA
                     GAPSSEFVAERIKESKTAGRVLGDGKRTFDEKLPRYLHRLSMPTLLVWGKEDKLTPTA
                     QHQTWAKALPTATVRLFDKAGHLVLDEAPAAVDAIASF"
     gene            3656..4459
                     /locus_tag="VM692_12060"
     CDS             3656..4459
                     /locus_tag="VM692_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ52950.1"
                     /translation="MIVRRVLAVLLIAYVAICAAVFLGQRRLQYFPDPLPVNAAAAGL
                     SEAESLTLATEDGERLVVWWVAPRDGQPVYLYLPGNGANLGNRGTRFARLVAGGAGLY
                     ALSWRGYGGSTGSPTEAGLMADARAAYADLTQRVAADRVVLYGESLGTTVAVMLAAEQ
                     PVRALLLDSSFDSALDVARWRFPWLPTTLLLRDTYRADLAAQRVAAPVLQVHCADDPI
                     TPLTSAERLHERFPHGQPLYRVDKRCHVVPLADYDAARDTFIRAVFTGG"
     gene            complement(4468..>5938)
                     /locus_tag="VM692_12065"
     CDS             complement(4468..>5938)
                     /locus_tag="VM692_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HUQ52951.1"
                     /translation="QVFFHKLGTPQSADTLVFEDAKNPQRFHTTSTTEDERFLILEVS
                     DRGKGLDGNALYVRDLKDANGKFAPLVPEVTNDTFYVVDNVGDQVFVATNHGAPNWRV
                     VQIDPRNIAEASWKTILAERPEPIDSVNMAGGKLFATYLKDVATRAYVFGLDGKLENE
                     IALPGPGTAAGFDGPNDAKEIYYTFNSLSVPTTIYRYDIATRASSVFRAPRVPGYDAS
                     QFETKQVFYPSKDGTRIPMFLVYRKGLKLDGNNPTLLYGYGGFNIVVSPTFSAARIAI
                     LEQGFVYASANLRGGGEYGETWHQQGMKLAKQNVFDDFIAAAEWLIEEKYTSSAKLAI
                     QGGSNGGLLVGAVMNQRPELFRVGIPQVGVMDMLRFHKFTIGWNWIADYGSSDDAQEF
                     KALYAYSPLHNIRAGVKYPATLITTADHDDRVVPAHSFKYAATLQQLASPDNPVLIRI
                     ETKSGHGASSLTKQLETTADIYAFTMHNLGVTPSGPLPR"
BASE COUNT          946 a         1984 c         1952 g         1056 t
ORIGIN      
        1 tcggcttgag cctcgaagcg ctgtgtgaga ttcacctgat agcaatcgcc gcggcgaatg
       61 tgatcttgca ccgctcggaa cgcggccgcg tactggccgc gatcgagatt cggccgcggc
      121 ggctgcaaca cgcgaaaggc ctgcggctgt ggcggcggct cggccgacaa gcgcgcgatg
      181 agctcgggcc actgcgcgaa cgtgcgcgcg tcgcggccgt tgccgacgag ccaggtgcgg
      241 cgcgcagcat gatcgacgac gacggcccag tcgtagagcc cgatcgcgag atccggcatc
      301 gcaatgtcgg ccgctgcgat cgacggaatg cgctcgagcc ttctgcccaa gtcgtagccg
      361 aagtaaccga ccgcgccgcc cgcgaacggc aagccctccg ctccgctgct gacttcgccg
      421 agctgctcgc gcacgagctt gaacggtgaa cgctgcgagc ggctcctcgc accgtcgcgc
      481 ttcgttacct ccgtggtcgc gccgcgcgtt cgcagcgtga cgtacggatc ggcgacgagg
      541 atgtcgaacc gatcgccggc gttgcccgcg gcgctatcga gccacgttgc ccatggcaac
      601 gcggccagcg tgcggaaacg ccgcgcgacc tcgtcccaat gagcaagctc gatgattctt
      661 ggcgtcgaca acgaactgaa cctctcccgc ggctcggcgg gagggggaca ttctagtgca
      721 gcgagcgtcg agcgctactt ttcgagcaag ccctcgacgt cggacaagac gtcctcgtcg
      781 ctctcgctcg ccgagcgggc cgccgcgtcg accttcggca ggtcgcgcaa cgtcgcctcc
      841 gtaagctcgc cgatgcgagc ggccgggttc tccatgatgg cgtcgtaacc ggcaatggaa
      901 ccgcgccgca tgacgggcgg cacgaagaac gcctcgatgt tcgccgtcgc accgtcgatg
      961 acgctgaccg agatcaacgt ggtcgagccc gacgagccga accccgtgag gatcgagacg
     1021 gccgaggcac cggcagcagc ggcataaatt tgaatttccg cgaaaccgac ggcgtcgacg
     1081 ccgagcttcg ccgccaacgc gcgcatctcg tcgccggcct cgtagcggcg cttgccgatc
     1141 tgtcgcggta aacgcaggcg cagctgcgtc agcatctcgt tgtaccgccg gcttgcgtcg
     1201 agcacgagct cttggagctc gggatcggcg ctgacgtcgt tcggtgtcag cacgcgaacg
     1261 tcgtagcctt gctccttgaa cgcggccagg acactcgcgc ccaggtaatt cgcgagctcg
     1321 ccgctctcct cgatctgctg ctcggtctgg acgaccttct tcttgatcag catggcctgc
     1381 ggcggcagaa atgcgatcgt tcgcgggcgc gtggtgcccg actcgaactc ttgtgtcgcg
     1441 tgcatcgcgg cgtcggccgt cgcggcagcg ccgctcagca cgctcgcagc gagcgcgtta
     1501 attagaagtc gtcgcagcat tgccagtctc ctcggtacgt aattgatcgc gcaacgtacg
     1561 cagccgcccc acgacgaccg tacgatcgga cgcgttgggc gccttgcgca ggtactcgac
     1621 gtaggcagcg gcagcggcgc gcggatcgcc gagccgctcc tcgacttcgc cgagcccgcg
     1681 atacgcgggc gcgaaattgg cgtcgagcgc gagcgcacgc tcgtacgacg cacgcgcctg
     1741 cccaagattc tcacgcagcg cggcctgccc ttcgggcgtc ttcaatagct gcgcttcgcg
     1801 ctcctgccgc gtccgcatga ctctgcggtt ggcgttgcgc gctcgctcgc ggttgctgag
     1861 ctcttcttcg tcgaactgcg tcagcgcgcc catggcggcc catgcgtcgc ccgtcagctg
     1921 cacgagctcc ggatgctcgg ggtagcgcgc cgcaagattc tcggcgagcg ccatcgccgt
     1981 gcgcggatag tcgtcctgga cgtaatcgcg aatcgtcatc gtgcggaccg ggaagacgac
     2041 cgcgtcgaac tgctcggtat cacgacggcc gtccggtgcg ccggcgactt gctcgcgcgt
     2101 gcgctcggcg cgcgcctcga gctgcgggtg cgtgctccag attgtcggga tgcgcggcgt
     2161 gatgccttcg taatcgcgcg cgaggacgcc gtagatctcc ggcaacgcct tcgcgtcgta
     2221 agggctcgca agcaacaggt cgacagcatg atcgtcggct tcttgctcga gctcgcggct
     2281 gaagccgtac atcgatgcgg caagaccgct cgagatgacg ctgccaagcc cgccgaacac
     2341 gcccgtcaac accatgctgg cgacgatttt tttgttcgcg gagcgaaagc tgagaatgcc
     2401 gtggtggcct gcgacgtgat tgacctcgtg ccccaagatc gcggcgagct gcgccgtgtc
     2461 cgtcagccgc gcgagcatgc cggtgtgtat gtagatgtcg ccgttcggca gcgcgaacgc
     2521 attcggcgac gggtcgcgca acacgaagaa tcgatattgg acgtagtcgt ccttgaccgc
     2581 cggcgcgagg cggcggccga cgctcgcgac gagctcgacg acacgcgggt cgttgtagag
     2641 cagcgactgg cgctcgaagt agccgtgcag ctcgcgcgat tcgtcgatga gccgctgctc
     2701 ctgatccttc aacggcactg gctcgtagac gccgtccgca agagcggtgg cggccgagag
     2761 tgcagccgca gctgtcagca gagctcgcat caggtatcgg ggcttccgag tcatgggcaa
     2821 cgcgtgtacg gtgactggcc tattagaaag caaacaaggg gcgcaaattg tgactcactt
     2881 cactcggcgc acgttacggg tcaatgggat cgacacggtc gtgctcgaag ccgggagcgg
     2941 cccgccactc gtgtacttgc acggcgcggg cactgtgacg ggcttcgact ttgccgaagc
     3001 ctggagccgc acgttccgcg tcgtcatccc gtaccacccc ggcttcggcc tgtcggccga
     3061 cgacccggcc gtcgacgaga ttcacgacta cgtgctgcac tacctggagc tgttcgacac
     3121 gctcgagctg cgacagtttc ggctcgtcgg ccagtccatg ggcgggttca tcgcgacgaa
     3181 gttcgccatc gagcacggcg agcgcatcga aaagctcgtg ctcgtctgcc cgatcgggct
     3241 accggtgccg cccggcatgc agaccgtgaa cttcctcgcc gtgccgcccg aggagctgcc
     3301 ggcactgctc gcatacgacc cgcagaccgt catcaagcat ctaccggccg gagcgccgag
     3361 cagcgagttc gtcgccgagc gaatcaaaga atcgaagacg gccgggcgcg tgctcggcga
     3421 cggcaagcgc acgttcgacg agaagctgcc gcgctacttg caccgcttgt cgatgccgac
     3481 gctgctcgtc tggggcaaag aggacaagct cacgccgacg gcgcagcatc aaacgtgggc
     3541 caaggcgctg ccgacggcca ccgtgcgcct gttcgacaaa gccggccacc tcgtgctcga
     3601 cgaagcgccg gccgccgtcg acgcgatcgc aagcttttaa cccagaagcg ggtcgatgat
     3661 cgtacggcgc gtgctcgcgg tactgctcat tgcctacgtc gcgatatgcg ccgcggtgtt
     3721 cctcgggcaa cgccgactgc agtacttccc cgatccactc cccgtgaatg cggcggccgc
     3781 cggtctgtcg gaagcggaat cgttgacgct cgccaccgag gacggcgaac ggctcgtcgt
     3841 ttggtgggtg gcgccgcgtg acgggcagcc cgtttacctc tacttgcccg gtaacggcgc
     3901 taacctgggc aaccgcggga cgcgcttcgc gcgattggtc gcgggcggtg ccgggctcta
     3961 cgccttgagc tggcggggtt acggcggctc gacgggttcg ccgaccgagg cgggactcat
     4021 ggccgatgcg cgtgccgcct atgcagacct cacgcaacgc gtcgccgccg accgcgtcgt
     4081 gctgtacggc gagtcgctcg gcacgaccgt cgccgtcatg ctcgcggccg agcaaccggt
     4141 gcgcgctctg ctgctcgatt cgtcgttcga ttcggcgctc gacgtcgcgc gctggcgttt
     4201 cccgtggttg ccgacaacgc tactgctgcg cgatacctac cgcgccgatc tcgcggcgca
     4261 gcgcgtcgcg gccccggtgc tccaagtgca ttgcgccgac gatccgatca cgcctttgac
     4321 ttcggctgag cggctgcacg aacgctttcc gcacgggcag ccgttgtacc gcgtcgacaa
     4381 gcgctgccat gtcgtgccgc tcgccgacta tgacgccgcg cgcgacacct tcatccgcgc
     4441 cgtgttcacg ggcggctgac gaatccttca gcgcggcagc ggcccgctcg gcgtcacgcc
     4501 gaggttgtgc atcgtgaacg cgtagatgtc ggccgtggtc tccagctgct tcgtgaggct
     4561 gctcgcgcca tggcccgatt tcgtctcgat gcggatcagc accgggttat cggggctcgc
     4621 aagttgctgc agtgtcgcgg cgtacttgaa cgagtgcgcc ggcacgacgc ggtcgtcgtg
     4681 atccgcggtc gtgatcagcg tcgcgggata cttcacgccg gcgcggatgt tgtgcagcgg
     4741 cgagtacgcg tacagcgcct tgaactcctg cgcgtcgtcg ctcgagccgt aatccgcgat
     4801 ccagttccag ccgatcgtga acttgtggaa gcgcagcatg tccatgacgc cgacctgcgg
     4861 aatgccgacg cggaacagct cgggccgctg attcatcaca gcgccgacga gtagcccgcc
     4921 gttcgaaccg ccttggatcg cgagcttcgc gctcgacgtg tacttctctt cgatcagcca
     4981 ctcggcggcc gcgatgaagt cgtcgaagac gttttgcttc gcgagcttca tgccctgctg
     5041 gtgccacgtc tcgccatact cgccgccgcc gcgcaagttc gccgacgcgt agacgaagcc
     5101 ttgctcgagt atcgcgatgc gcgccgcgct gaacgtcggc gacacgacga tgttgaagcc
     5161 gccgtagccg tacagcagcg tcgggttgtt gccgtcgagc ttcaggcctt tgcggtagac
     5221 gaggaacatc gggatccgcg tgccgtcctt gctcggatag aagacctgct tcgtctcgaa
     5281 ctgcgaggcg tcgtagccgg gcacgcgcgg tgcgcgaaac acgctgctcg cgcgggtcgc
     5341 gatgtcgtag cggtaaatcg tcgtcggcac gctcagcgaa ttgaacgtgt agtagatctc
     5401 cttcgcgtcg ttgggcccgt cgaacccggc ggccgtgccg ggccccggca gcgcgatctc
     5461 gttctcgagc ttgccgtcga gcccgaacac gtatgcgcgc gtcgcgacgt ccttcaaata
     5521 ggtcgcgaac agcttacctc ccgccatgtt cacgctgtcg atcggctccg ggcgctccgc
     5581 gagaatcgtt ttccagctcg cctcggcgat gttgcgcgga tcgatctgca cgacacgcca
     5641 gttcggcgcg ccgtgattcg tcgcgacgaa gacctggtcg ccgacgttgt cgacgacgta
     5701 gaacgtgtcg ttcgtgacct cgggcacgag cggcgcgaac ttgccgttgg catccttgag
     5761 atcgcgcacg taaagcgcgt tgccgtcgag gcccttgccg cgatccgaca cctcgaggat
     5821 caggaagcgc tcgtcctcgg tcgtcgacgt cgtgtggaaa cgctgcggat tcttggcatc
     5881 ctcgaagacg agcgtgtcgg ccgactgcgg cgtgccgagc ttgtgaaaga agacctga
//
LOCUS       DATLEE010000558         4172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1397534, whole genome shotgun sequence.
ACCESSION   DATLEE010000558 DATLEE010000000
VERSION     DATLEE010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397534"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..577
                     /gene="pilV"
                     /locus_tag="VM692_12070"
     CDS             113..577
                     /gene="pilV"
                     /locus_tag="VM692_12070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HUQ52952.1"
                     /translation="MTTHITIRKSHAGFGLIESLVALIVISVGMIGIAALYGQGLRAS
                     GTALYRTMAVNLASDMADRIRVNRRGNSSYGAAAANGNCDPGGNTDCTVAAMARHDLF
                     VWQAQVAAQLPNGAGVVQFAGTTPPTYTIRVTWQEVGAGALTHQLVVRIPNL"
     gene            586..1575
                     /locus_tag="VM692_12075"
     CDS             586..1575
                     /locus_tag="VM692_12075"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HUQ52953.1"
                     /translation="MRAHKEHGMTLIELMVSLAIGSFLMLGAITVFMQSRTTFRVTES
                     LARMQENARFALDVLEPEIRMAHFWGLHTTTSYVFGAAAPTAANGPGPDTCGQNWTIN
                     LFEAVQGSNNSYGFGCAGTAPVETNADTLIVRRAAEDVETPPLTGAATLRIMSRRAPA
                     LSPIFTGTALPAGYSAATSEIHRMIANGYYVSRTSALTNGANLVPSLRVWTLLNTGVM
                     QNQEVIAGVEDFQVQFGVDTDLVDTADRGSVDRWVNVNDPMITPGAGGFNPDAVVLAV
                     RVWVRVRAERPENGFVDNVNYVYADQNVGPFNDNFRRLVVSKTIYLRNARPIS"
     gene            1587..2027
                     /locus_tag="VM692_12080"
     CDS             1587..2027
                     /locus_tag="VM692_12080"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HUQ52954.1"
                     /translation="MKTRHSFPQTRPALRSAQTGATLIVGLVLLLVLTVVGVSGMNTA
                     TMEITMAANTQFQQDSFQQAEDAIDIALATRAYSTDAPRTVNWLGNPDYDRRAVTTYR
                     MNTPAPGNSFDYEAFHFDIQSVGRGPRNALSQHTQSFYVVGRAL"
     gene            2047..2403
                     /locus_tag="VM692_12085"
     CDS             2047..2403
                     /locus_tag="VM692_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52955.1"
                     /translation="MARKLLVTLGLLLLTHSAAAVRVIEQVERPVELTLAELNLPAAG
                     GTTISFSACPTCSTSTHRLLDTTVLKVNGQTVPLQEFIRLADEIGDKPNGAENAIAVV
                     FLDLATERITRIEVRE"
     gene            2540..>4172
                     /locus_tag="VM692_12090"
     CDS             2540..>4172
                     /locus_tag="VM692_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUQ52956.1"
                     /translation="MADDTELFIGTTLNSTAKPNILFIIDNSGSMATLVLTQETYNGG
                     TTYPALGGCDATRVYWRTGTGNPPACTTNNYFNGAALKCDRAAQAFLTAGYYTDNMAQ
                     YDPSNGSGGKRWETIAAAQKTRVVECQDDRGVHGDGVNMTNLYARNGGTTAGYWGTSG
                     QEIAWGAAPANETYTLYSGNYLNWMAGPTAFSTRLEIVQDVATDLLDSINGVNVGLAY
                     FNRNTDNSNNGGRIAYAVEDIGTARAPIQAAVNALAPDGNTPLSETLYEAAQYFSGRR
                     VVYGDPNRSVAASREPTDATLYNSPMNYDCQKNFIVLLTDGEPTQDSAADAAIRGMTD
                     GNGGSFSSLVGGTCDTEVYPVGFNPSGGQCLDDLAELLHDGDFSAMTGQQGVTTYTVG
                     FTIDLPILAETAQRGGGRYYTADDTATLAGALTSIVTAILTEDTTFTAPTVAVNSFNR
                     TQNLSDLFISVFRPSGRTHWPGNLKKYRIRASDATIVDANGNPAIDPATGFFSQTARS
                     YWSSVVDGRVADVGGAADLVPGSTSRVVYTQLSGND"
BASE COUNT          815 a         1395 c         1276 g          686 t
ORIGIN      
        1 cgcgtcgtgc gcatcgatcg ccccggtcgc gggatggtat tgcaggagaa gacggccatc
       61 gatcccctca tcgctggcgt cggagctacg tgcccataag aggcaacggt aaatgacaac
      121 gcacatcacg attagaaaat cccacgccgg cttcgggttg atcgagtcgc tcgtcgcgct
      181 gatcgtcatc tcggtcggca tgatcgggat cgcggcgctc tacggccagg gtctacgcgc
      241 gagcggcacc gcgctctacc gaaccatggc cgtcaacctc gcttccgaca tggcggaccg
      301 gattcgcgtc aatcgccgcg gcaactcgag ctacggcgcg gccgcggcga acggcaactg
      361 cgatcccggc ggcaatacgg attgcacggt cgcggcgatg gcaagacacg atttgttcgt
      421 gtggcaggcg caagtcgcgg cgcagctgcc gaacggggcc ggcgtcgtgc agttcgccgg
      481 cacgactccg ccgacctaca cgatccgcgt gacatggcag gaggtcggcg ccggcgcgct
      541 gacgcaccag ctcgtcgtgc gcatcccgaa cctgtgagcc gaacgatgag agcacacaaa
      601 gaacacggta tgaccctgat cgagctgatg gtgtcgctcg ccatcggctc gtttctcatg
      661 ctcggcgcca taacggtctt catgcagagc cgcacgacgt ttcgagtgac ggaatccctc
      721 gcgcggatgc aagaaaacgc gcgcttcgcg ctcgacgtgc tcgagcccga gattcgcatg
      781 gcgcatttct gggggcttca cacaaccacg tcatatgtct ttggcgccgc tgcgccgacg
      841 gccgccaacg gcccgggacc ggatacttgc ggccagaatt ggacgatcaa tctgtttgag
      901 gccgtacagg gcagtaacaa cagctacggc ttcggttgcg cgggcacggc gcctgttgaa
      961 accaacgccg atacactgat cgtccgccgc gccgccgagg acgtcgagac gccgccgctg
     1021 acgggtgctg caacgctgcg aatcatgagc aggcgcgctc cggccctgtc gccgattttc
     1081 accggcacgg cactccccgc tggttatagc gcggcgacga gcgagatcca ccgcatgatt
     1141 gccaacggct actacgtcag ccgcacttcg gcgctgacca acggggcgaa cttggttccc
     1201 tcgctgcgcg tgtggacgct actgaacacc ggcgtgatgc aaaaccaaga ggtcatcgcc
     1261 ggcgtcgaag actttcaggt gcagttcgga gtcgatacgg atttagtcga caccgccgat
     1321 cgcggctcgg tcgaccgctg ggtcaacgtc aacgacccga tgatcacgcc cggagccggc
     1381 ggctttaacc ccgacgccgt agttctcgcg gtccgcgtct gggtccgcgt gcgcgcggag
     1441 cggcccgaga acgggttcgt cgacaacgtc aactacgttt acgcggacca gaacgtcggc
     1501 cccttcaacg acaatttccg ccgtctcgtc gtctcgaaaa cgatctacct gcgtaacgcc
     1561 cggccgatct cgtgaggatt acaacaatga agacgcgtca ctcgttcccg cagactcgcc
     1621 ccgcgctgcg cagcgcgcag acgggcgcca cgctgatcgt cggcctcgtg ctgttgctcg
     1681 tgctgacggt cgtcggcgtg tccggcatga ataccgccac gatggaaatc accatggcgg
     1741 ccaatacgca attccagcag gactcgttcc agcaagccga ggacgcgatc gacatcgcgc
     1801 tcgccacgcg tgcgtactcg acagatgcgc cgcgcaccgt caactggctc ggcaatcccg
     1861 attacgaccg ccgcgcggtc acgacatatc gcatgaacac tcccgcgccg ggcaacagct
     1921 tcgattacga agcctttcat ttcgacatcc agtccgttgg ccgcggcccg cgcaatgcac
     1981 tgtcacaaca tacgcaaagt ttctacgtcg tcggacgcgc cctctaacgg gcttcggaga
     2041 actcacatgg ctcgcaaact actcgtcacg ctcggtctct tgttgctgac tcactcggcc
     2101 gcggccgtgc gcgtcatcga gcaagtcgaa cggccggtcg agcttacgct cgccgagctg
     2161 aacttgccgg cggcgggcgg caccaccatc agctttagcg cgtgcccgac gtgcagcacc
     2221 agcacccatc ggctattgga cacgacggta ttgaaggtga acggccagac cgtgccgctc
     2281 caggaattca tccgcctcgc cgacgaaatc ggcgataagc cgaacggcgc cgagaacgcg
     2341 atcgccgtgg tgtttctcga tctcgcaacg gagcggatca cgcgcatcga agtgcgcgag
     2401 taatacgcga cagagtctcc atcgctacgc cgggacggcg caccgcaacc tgaaggacat
     2461 cgatatgaat ctcagcaaga cacgcatgag ttggctcgga ctgggcgccc tgtgggcggt
     2521 tgcttcggcg cttcccgcaa tggccgacga caccgagctc ttcatcggga cgacgctgaa
     2581 ctcgacggcg aagccgaaca ttctgttcat cattgacaac tcgggcagca tggccacgct
     2641 cgtgctgacc caagagactt acaacggtgg gacgacctac ccggcgctcg gcggctgcga
     2701 cgccacgcgc gtttactggc gcaccggcac gggcaatccg ccggcgtgca cgaccaataa
     2761 ctatttcaat ggtgccgcgc tgaaatgcga ccgggctgct caggcgttcc tgacggccgg
     2821 ctattacacg gacaacatgg cgcagtacga cccgagcaac ggcagcggcg gcaaacgttg
     2881 ggaaaccatc gccgccgcgc agaagacccg cgtggtcgag tgtcaagacg accgcggcgt
     2941 tcatggcgat ggcgtcaaca tgaccaacct ctacgcgcgc aacggcggaa cgacggcggg
     3001 ttactggggc acgtccgggc aagaaatcgc ctggggcgca gctccggcga acgagaccta
     3061 cacgctctat agcggcaact atctgaactg gatggccggt ccgactgcgt tcagcacgcg
     3121 tctcgagatc gtccaagacg ttgcaacgga tttgctcgac tcgatcaacg gcgtgaacgt
     3181 cggcctcgct tatttcaacc gcaacacgga caactcgaac aacggcgggc gtatcgccta
     3241 cgcggtcgag gacatcggga cggcgcgcgc tccgatccag gccgcagtca acgcgctggc
     3301 gccggacggc aacacgccgc tctcggagac tctctacgag gccgcgcaat acttttcggg
     3361 ccgccgtgtc gtctacggcg atccgaacag gagcgtggcc gcttcacgcg aaccgacgga
     3421 cgcgacgctc tataactcgc cgatgaacta tgactgtcag aagaacttca tcgtgctcct
     3481 gaccgacggc gagccgacgc aagacagcgc cgccgatgcg gcgatccgcg gcatgacgga
     3541 cggcaatggc ggcagcttct cgagcctcgt cggagggacc tgcgataccg aggtttaccc
     3601 cgtcggcttc aacccgtccg gcggacagtg cctcgacgat ctcgccgagc tcctacacga
     3661 cggcgacttc tcggcgatga cgggtcagca gggcgtcacc acctacaccg tcggcttcac
     3721 gatcgacctg ccaatccttg cggaaacggc gcagcgcggc ggcggccgtt actacacggc
     3781 cgatgacacg gcgacgctgg ccggcgcgtt gacgagcatc gtcaccgcga tcttgacgga
     3841 agatacgacg ttcacggcgc cgaccgtcgc ggtgaactcg ttcaaccgca cgcaaaacct
     3901 gagcgacctc ttcatcagcg tgttccgccc atcgggccgc acgcactggc ccggcaactt
     3961 gaagaagtac cgcatccgcg cgagcgacgc gacgatcgtc gacgccaacg gcaacccggc
     4021 catcgacccg gcgacgggct tcttctcgca gaccgcgagg agctactggt cgtccgtcgt
     4081 cgacggccgc gtggccgacg tgggtggcgc cgccgatctc gtgccgggct cgacgagccg
     4141 agtcgtctac acgcagctga gcggcaacga ct
//
LOCUS       DATLEE010000559         2330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3322369, whole genome shotgun sequence.
ACCESSION   DATLEE010000559 DATLEE010000000
VERSION     DATLEE010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3322369"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VM692_12095"
     CDS             <1..950
                     /locus_tag="VM692_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005190612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HUQ52957.1"
                     /translation="ATPSANRITLLRDSNRDGEPELRTTFVDGLNSPFGMTLVGDDFY
                     VANTDALVRFAYTSGTTRLAPAGERVAELPGGEINHHWTKNVIANRDGTQLYVTVGSN
                     SNVAERGLEAEQQRAAILEIDAATGNTRVYASGLRNPNGLAWEPRTGALWTVVNERDE
                     IGSDLVPDYLTSVVRDGFYGWPYSYFGGNVDRRVEPGNPELVAKARVPDYALGPHTAS
                     LGLLFYRGEALADYRGGAFIAQHGSWNRKPRSGYRVIFVPFTDGRPQGEAREVLTGFL
                     TDEGEAKGRPVGLAVDATGALLVADDVGNTVWRVAPIAR"
     gene            1096..>2330
                     /locus_tag="VM692_12100"
     CDS             1096..>2330
                     /locus_tag="VM692_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HUQ52958.1"
                     /translation="MLLTLIVLLPFAGSLCTAFLPAHARNAAAWLAELVAVACVALTI
                     VHYGAVADGGVMRATLAWLPQYGLDFYLRMDGFAWLFALLVSFIGALVVLYARYYMSR
                     KDPVPRFFSFLLAFMGAMLGTVLSGNLIQLVVFWELTSLTSFMLISYWYHRADARRGA
                     RMAAIVTVGGGLCLLLGVLMLGRIVGSYDLDRVLAAGDVIRAHPWYAPTLVLVMLGVA
                     TKSAQFPFHFWLPQAMAAPTPVSAYLHSATMVKAGVFLLARLWPALAGTDLWFGLVCG
                     AGGMSLLLGTFSATYQRDMKGVLAYSTIGHLGLITLLLGLNTELALVAAVFHMLNHAT
                     FKASLFMATGVVDHETGTRDLKRLHGLRHAMPITAVLATVAAAAMAGVPLLNGFLSKE
                     MFFEETIVAGRGIWSYGLP"
BASE COUNT          345 a          764 c          774 g          447 t
ORIGIN      
        1 cggcgacgcc aagcgcgaac cgcatcacgc tgctgcgcga cagcaaccgc gacggcgagc
       61 ccgagctgcg cacgacgttc gtcgacgggt tgaactcacc gttcggcatg acactcgtcg
      121 gcgacgattt ttacgtcgcg aacacggacg cgctcgtgcg cttcgcgtac acgagcggta
      181 cgacgaggct cgctccggcc ggcgagcgcg tcgccgagtt gccgggcggc gagatcaatc
      241 accactggac gaagaacgtc atcgcgaacc gcgacggcac gcagctctat gtgaccgtcg
      301 gctccaacag caacgtcgcc gagcgcggcc ttgaagcgga gcaacagcgc gcggcgatcc
      361 tcgagatcga cgcggcgacg ggcaacaccc gcgtctatgc atcggggttg cgcaatccga
      421 acggcctcgc ttgggagccg cgcacgggtg cgttgtggac cgtcgtcaac gagcgcgacg
      481 agatcggcag cgatctcgtg ccggactatc taacgtccgt cgtgcgcgac ggcttctacg
      541 gttggcccta cagctatttc ggcgggaacg tcgataggcg cgtcgagccc ggtaaccccg
      601 agctcgtcgc caaggcgcgc gtccctgact acgcgctcgg gccgcatacg gcatcgctgg
      661 gccttctgtt ctaccgcggc gaggcgctcg ccgactatcg cggcggcgca ttcatcgcgc
      721 agcacggctc gtggaatcgc aagccgcggt caggctatcg cgtcatcttc gtgccgttca
      781 cggacggccg gccgcaaggc gaggcccgtg aggtgctgac gggctttctg acggacgaag
      841 gcgaggcgaa gggccggccc gtcggtctcg ccgtcgacgc cacgggtgcg ctgctcgtcg
      901 ccgacgacgt cggcaacacc gtttggcgag tcgcgccgat cgcgcgctga gcaagcggct
      961 ttcaataggg cctgagcgcc gcctgcagta attcttgtta agctcgcccg atcgcacgcg
     1021 cagtcgtcgc gatacccaca gtgacgagcg accgatgaag agaccagcgt tcgatccgac
     1081 gcggcggggc gctcgatgct cctgaccctc atcgtacttc tgccgtttgc cggcagcttg
     1141 tgcacggcgt ttctgccggc tcacgcccgc aatgcggcgg cctggctcgc ggagctcgtc
     1201 gcggtcgcct gcgtcgcgct gacgatcgtg cactacggcg ccgtcgcgga cggcggcgtc
     1261 atgcgtgcaa cgcttgcgtg gctgccgcag tacggcctcg atttttacct gcgcatggac
     1321 ggtttcgcgt ggctgttcgc gctgctcgta tcgttcatcg gcgcactcgt cgtgctctac
     1381 gcgcgctatt acatgtcgcg aaaggacccg gtcccgcggt tcttctcgtt tctcttggct
     1441 ttcatgggcg cgatgttggg caccgtgctc tccggcaatc tgattcagct cgtcgtcttc
     1501 tgggagctca cgagcctgac ctcgttcatg ctgatctcgt attggtacca tcgcgccgac
     1561 gcgcgacgtg gcgcacgaat ggccgccatc gtgaccgtcg gcggtgggct ctgccttttg
     1621 ctcggcgtgc tcatgctcgg caggatcgtc ggcagctacg acctcgacag agtgctggcg
     1681 gcaggcgacg tcatacgggc gcatccgtgg tatgcgccga cgctcgtgct cgtgatgctg
     1741 ggcgtcgcaa cgaagagcgc gcagttcccg ttccatttct ggctgccgca ggcgatggcg
     1801 gcgccgacgc ccgtctcggc gtacctgcat tccgccacga tggtcaaggc gggcgtgttc
     1861 ttgttggcgc gtttgtggcc ggctctcgcg ggcaccgacc tgtggttcgg tctcgtctgc
     1921 ggtgcaggcg gcatgtcgtt gctgctcggc acgttcagtg ccacttatca gcgcgacatg
     1981 aagggcgtgc tcgcatactc cacgatcggc cacctcggcc tgatcactct gttgctcggc
     2041 ctgaataccg agctcgcctt ggtagccgcc gtcttccaca tgctgaacca cgcgacgttc
     2101 aaagcgtcgc tgttcatggc cacgggcgtc gtggatcacg aaaccggaac gcgcgatctg
     2161 aagcgcctac acgggttacg gcatgcgatg ccgatcacgg ctgtgctcgc gaccgttgcg
     2221 gcggccgcga tggccggcgt gccgctgctg aacgggttct tgtcgaagga gatgttcttc
     2281 gaggagacga tcgtggccgg caggggcatc tggtcgtacg gcctgccgtt
//
LOCUS       DATLEE010000560         2683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10017884, whole genome shotgun sequence.
ACCESSION   DATLEE010000560 DATLEE010000000
VERSION     DATLEE010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10017884"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VM692_12105"
     CDS             complement(<1..428)
                     /locus_tag="VM692_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HUQ52959.1"
                     /translation="MIIRILIAALLVLVLPHAALAQSLPAVTVDQLANGGQSYSLSIQ
                     VLALMTALTVLPALVLSMTAFTRIVIVLAILRQALGTPQTPPNQVLIGLALFLTLFVM
                     TPVMTRAYDEAVVPYMAGEQDAMTAARNGLVPLREFMLDQV"
     gene            complement(425..805)
                     /gene="fliO"
                     /locus_tag="VM692_12110"
     CDS             complement(425..805)
                     /gene="fliO"
                     /locus_tag="VM692_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HUQ52960.1"
                     /translation="MSAGRTLAVALPSVLAAATAHAATPTVGMGDLSGVILSLVLVVG
                     FILAAAWVVRRMPLGLGRGNGPLKVLAALPLGPRERLVLVEARGEELLIAVSPAGVFN
                     VGAAQGKPTAARAPEPTFVLPDPT"
     gene            complement(802..1065)
                     /gene="fliN"
                     /locus_tag="VM692_12115"
                     /pseudo
     CDS             complement(802..1065)
                     /gene="fliN"
                     /locus_tag="VM692_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018879075.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
     gene            complement(1137..2141)
                     /locus_tag="VM692_12120"
     CDS             complement(1137..2141)
                     /locus_tag="VM692_12120"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HUQ52961.1"
                     /translation="MAADSPKAAAAPRADITGAEVDALLVKDSAAAAPAGTPQPYDLV
                     ARDRIVRGRMPVLDRLNERWVTDFERKLGELIRQPLEVTLQEVQLAPYGNWLAALPVP
                     TSFNLYTVKPWPRNALVAVDGKLLFALVNSYYGGTRAKPNAARETLTPTEQRLNKIVI
                     EMLVALFRSALSPVATLEFQHVQSDVNASYLNMATPSETVVVTRVEVTLKEQGGSIAL
                     LLPLSSFDPVRDKLAEGLKTVSAETLKRWRDGLRAQLEHTELDLTSVFLETEVSMREL
                     LQMKPGDILPIEMPKTALLRSGSRPLLRGKFGLSRGYNAVSVLEAVKSPSPLSQEKPR
                     "
     gene            complement(2163..>2683)
                     /locus_tag="VM692_12125"
     CDS             complement(2163..>2683)
                     /locus_tag="VM692_12125"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HUQ52962.1"
                     /translation="LVVIGAVALFVALVGAQVAGPLINGMIAGASHAATADAETTDEA
                     AAEEELVLADANVEEEEEHEPLEPAQYVALDPPFVVSFEDDGGSRYLQLTLQAMARSE
                     ETIEAIKLHAPAIRNSILFRMSGYELAVLTNQAGKEQLRHDLLDAANEILVKNEVEGS
                     IEDLYFTSLVIQ"
BASE COUNT          446 a          899 c          892 g          446 t
ORIGIN      
        1 acttgatcga gcatgaactc gcgcagcgga acgagcccgt ttcttgcggc cgtcatcgcg
       61 tcttgctcgc cggccatata cggcacgacg gcctcgtcgt aggcgcgcgt catgacgggc
      121 gtcatgacga acagcgtcag aaacagagcg agaccgatca gcacttgatt cggcggcgtc
      181 tgcggcgtgc cgagcgcttg gcgcagaatc gcgagcacaa tgacgatgcg cgtgaacgcc
      241 gtcatcgaca gcacgagcgc cggcagcacc gtcagcgccg tcatcagcgc gagcacttgg
      301 atgctcagcg aatagctctg tccgccgttg gcgagctgat cgaccgtgac ggccggcagc
      361 gattgcgcga gcgcagcgtg cggcagcacc agcacgagca gcgcggcgat gagaatgcga
      421 atgatcatgt gggatccggt aacacgaacg tcggctccgg ggcccgcgca gccgtcggtt
      481 tgccttgcgc cgcgccgacg ttgaagacgc cggccgggct cacggcgatc agcagctcct
      541 cgccgcgcgc ctcgacgagc accaagcgct cgcgggggcc gagcggcaga gcagcgagga
      601 ccttcagggg gccgttgcca cgccccaagc cgagcggcat gcgccgcacg acccaggccg
      661 ccgcaagaat gaagccgacg acgagcacga ggctcaagat cacgcccgac aggtcgccca
      721 tcccgacagt cggcgtcgcg gcgtgcgccg tcgccgcggc gagcacgctc ggcaacgcga
      781 cagcaagtgt gcgcccggcg ctcattcctt cagcgactcc acgcgctttt cggggctcac
      841 gacgtcgagc aagcgaatcc cgaacttgtc gttgacgacg acgacctcgc cgtgcgccac
      901 gagcgtcccg ttgacgagca cgtcgagcgg ctcgccggcg agcttgtcga gctcgacgac
      961 ggaaccctgc gtcagcgaga gcaggttgcg tacgctgacg cgagtgcgtc cgacctcgag
     1021 cgtcagcgtc agccgcacgt ccatgatcag gtcgagattc agttggcctg ctgcgccttg
     1081 cgcggtgcgc ggcaactccg cgaactcggc cgcagcaacg ccggcttgat cagcgctcat
     1141 cgaggtttct cctgggaaag ggggcttggg ctcttgacgg cttcgagcac gctcacggcg
     1201 ttgtagccgc gggaaaggcc gaacttgcca cgcaacagcg gtcgagaccc cgagcgcagc
     1261 agtgcggtct tcggcatttc gatcggcaga atgtcgccgg gcttcatctg caacagctcg
     1321 cgcatgctga cctcggtctc gaggaacacg ctcgtcagat cgagctccgt atgctcgagc
     1381 tgcgcgcgca agccgtcacg ccagcgcttg agcgtttcgg cggaaacggt tttgagtccc
     1441 tcggcgagct tgtcgcgcac gggatcgaac gacgacagcg gcagcagcag cgcgatcgac
     1501 ccgccctgct ccttcagcgt cacctcgacg cgcgtcacca caaccgtctc gctcggcgtc
     1561 gccatgttca gatagctcgc attgacgtcg ctctgcacgt gctggaactc gagcgtcgcg
     1621 acgggcgaca gcgcgctgcg aaacagcgcg acgagcatct cgatgacgat cttgttgagc
     1681 cgctgctccg tcggcgtcag cgtctcgcgt gcggcgttcg gcttcgcgcg cgtgccgccg
     1741 tagtagctgt tgacgagcgc gaacaagagc ttgccgtcga cggccacgag cgcgttgcgc
     1801 ggccacggct tgaccgtgta cagattgaag ctcgtcggca ccggcagcgc cgcgagccaa
     1861 ttgccgtacg gcgcgagctg cacttcttgc agcgtcacct cgagcggctg acgaatcagc
     1921 tcgccgagct tgcgttcgaa atccgtgacc caacgctcgt tgagccggtc gagtaccggc
     1981 atgcgcccgc gcacgatgcg atcgcgcgcg accaggtcgt acggttgcgg cgtgccggcc
     2041 ggggcggcgg ctgcggaatc tttgaccaac agcgcgtcga cctcggcacc ggtgatgtcg
     2101 gcgcgaggcg cggctgctgc cttcggggag tcggctgcca tgtcgttgct tcggtgagtc
     2161 gattactgga tgacgaggct cgtgaagtac aggtcctcga tgctgccctc gacctcgttc
     2221 ttgacgagga tctcgttggc cgcatcgagc aggtcgtgcc gcagctgctc tttgccggcc
     2281 tggttcgtca gcacggcgag ctcgtagccg gacatcctga acaggatgga attgcggatc
     2341 gccggcgcgt gcagcttgat cgcttcgatc gtctcctcgc tgcgggccat cgcttgcagc
     2401 gtcagctgca gatagcgcga gccgccgtcg tcctcgaagc tgacgacgaa cggcgggtcc
     2461 agcgcgacat actgcgcggg ctcgagcggc tcgtgctctt cctcttcctc gacgttggcg
     2521 tccgcaagca cgagctcctc ttcggcagcc gcctcgtcag tcgtctcggc atcggccgtc
     2581 gcggcatgcg aggctcccgc gatcatgccg ttgatcagcg gtcccgcgac ctgcgcgccg
     2641 acgagcgcga cgaacagcgc gacggcgccg atgacgacga gct
//
LOCUS       DATLEE010000561         4376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3324126, whole genome shotgun sequence.
ACCESSION   DATLEE010000561 DATLEE010000000
VERSION     DATLEE010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3324126"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VM692_12130"
     CDS             <1..471
                     /locus_tag="VM692_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUQ52963.1"
                     /translation="NASNFYPTPLGTITGEQWQDLMATNLKAPLFLSQALLPALRAAR
                     GVIINIVDVHSQRPLRDHPVYGAAKAGLAMLTRSLAKDLGPHVRVNGVSPGAILWPDE
                     GMSDALRAGIIKQTALKRSGEPEDIANTVLFLVRDAPYITGQIIAVDGGRSVGW"
     gene            complement(478..978)
                     /gene="folK"
                     /locus_tag="VM692_12135"
     CDS             complement(478..978)
                     /gene="folK"
                     /locus_tag="VM692_12135"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HUQ52964.1"
                     /translation="MPEVFVGAGSNFGPERALRRAGAELERRFGAVRYSRVYRSAAVG
                     ASAAPLGSPRADYLNLVVAFSTDRDADSVHDELRAIETLAGRTRIDRAVCELDLDLLL
                     YGASVDAARRLPRPGLFTAPFILGPLAELAPTLVHPVTAERCGVAWQAAKHDALRDVG
                     PFSALR"
     gene            complement(971..1333)
                     /locus_tag="VM692_12140"
     CDS             complement(971..1333)
                     /locus_tag="VM692_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HUQ52965.1"
                     /translation="MDKIFLRELRIETIIGFWEWERRIKQVVSIDLEIATDARVAAAS
                     DAIAGTLNYEQLAKRLIEFVGESQYQMVEALATAIGKIVIEEFGAPWVRVSVAKPGAI
                     SAARDVGIVIERTAADYA"
     gene            complement(1347..2372)
                     /gene="tsaD"
                     /locus_tag="VM692_12145"
     CDS             complement(1347..2372)
                     /gene="tsaD"
                     /locus_tag="VM692_12145"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041600021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HUQ52966.1"
                     /translation="MRVLGVETSCDETGVALYESGAGIRAERLASQIAIHAAYGGVVP
                     ELASRDHIAKLLPMVEDVLGEAGLTLRDLDAIAYTAGPGLIGALMVGGSLASSLAYGL
                     KIPAIPVHHLEGHLLACMLEESAPEFPFLALLVSGGHSQLIEVSGVGRYHIVGETLDD
                     AAGEAFDKVARLLGLPYPGGPQLAALAERGDSQRFSLPRPMKNQGLDMSFSGLKTAVR
                     LTIDSCRDSAGRLADRERADLAASFQAAVVDTLTGKCRQALEATGLQRLVIAGGVGAN
                     KALRASLAELGGRVGARVYYPRPALCTDNGAMIAYAGWCRRTTAGNGLPRIVAKPRWP
                     LGELTPP"
     gene            2482..2700
                     /gene="rpsU"
                     /locus_tag="VM692_12150"
     CDS             2482..2700
                     /gene="rpsU"
                     /locus_tag="VM692_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HUQ52967.1"
                     /translation="MPGVRIRESEHFDAALRRFKRACEKAGVLTELRRREFYEKPTQE
                     RKRKKAAAVKRHMKRNSRTPTTRRRRLY"
     gene            2719..3168
                     /locus_tag="VM692_12155"
     CDS             2719..3168
                     /locus_tag="VM692_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HUQ52968.1"
                     /translation="MSLKDRLTQDMQAAMKSGDKARLSVLRMALAGVKQREVDTRRPL
                     DDAGVQTVIERMIKQGKDALQQFRDGGRADLVAKEEGELKVLQTYLPQQLSDAELDTF
                     LAECIDAAGATSVKDLGKVMGVIKTRAAGRVDMSTVSARVRTLLTPE"
     gene            3232..>4376
                     /gene="dnaG"
                     /locus_tag="VM692_12160"
     CDS             3232..>4376
                     /gene="dnaG"
                     /locus_tag="VM692_12160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HUQ52969.1"
                     /translation="MAGRIPQAFIDELVARADIVEVVGARVTLKRAGSNYKGLCPFHG
                     EKTPSFTVSPSKGFYHCFGCSAHGTAIGFLMAYDNLEFPQAVEALAEMMGLEVPRENA
                     GQPEHRDENEELYALLREADQIYRAALRSSDTAIAYLKKRGIDGATAGRFGIGYAPNA
                     WDTVLRQLGTSDARVAKLLQAGLVVANESGRRYDRFRDRIMFPIRSARGHVIGFGGRV
                     LDSGEPKYLNSPETPIFRKGQELYGLYEARQNPGKPKSIIVVEGYLDVASLVQFGVEN
                     VVATLGTAMTAENMKRLTRLTDRVIFCFDGDRAGRAAAWRALETALPYGGGTFELKFL
                     LLPESDDPDSFVRSKGPDAFRALAETAAPLSDFLVKELTARVDLNSV"
BASE COUNT          795 a         1448 c         1437 g          696 t
ORIGIN      
        1 aacgcgtcaa atttttaccc gacgccgctc ggcacgatca cgggcgagca atggcaggac
       61 ctcatggcga cgaacttgaa ggcgccgcta tttctttcgc aggcgctgct gccggcgctg
      121 cgcgccgcgc gcggcgtcat catcaacatc gtcgacgtgc attcgcagcg gccgctgcgc
      181 gaccatcccg tctacggcgc cgcgaaggcc gggctcgcga tgctgacgcg gtcgctcgcc
      241 aaggatttgg ggccgcacgt gcgcgtcaac ggcgtctcgc cgggcgccat tctgtggccg
      301 gacgaaggaa tgtcggacgc gctgcgcgcc ggcatcatca agcaaacggc gttgaaacgc
      361 agcggcgagc ccgaggacat cgcgaacacc gtgttgttct tggtgcgcga tgcgccgtac
      421 atcacaggcc agatcatcgc cgtcgacggc gggcgcagcg tcggctggtg acgccgctca
      481 gcgcaacgcg ctgaacggac cgacgtcgcg caaggcgtcg tgcttggccg cctgccaagc
      541 aacgccgcac cgttcagccg tgacggggtg cacgagcgtt ggagcgagct cggcgagcgg
      601 acccaagatg aacggcgccg tgaacaatcc cggtcgaggc aaccgccgcg ccgcatcgac
      661 gctcgcgccg tacagcaaca gatccaagtc gagctcgcaa acggcacggt caatccgcgt
      721 gcggcccgcg agcgtttcga tcgcgcgcag ctcgtcgtgc acggagtcgg cgtcgcggtc
      781 ggtcgaaaag gccacgacca gattcaggta atcggcccgg ggactaccga ggggcgccgc
      841 cgaagcgccc acagccgcgc tgcgatatac gcgtgagtag cgaacggcgc cgaaccggcg
      901 ctcgagctcc gcgccggcgc ggcgtagcgc tcgctcggga ccgaagttac tgccggcccc
      961 gacgaaaacc tcaggcatag tcggcggcgg tgcgctctat gacgatgccg acatcgcgtg
     1021 cggcggagat cgcgccgggc ttcgcgaccg atacccttac ccatggcgcg ccgaattctt
     1081 caatgacgat cttgccgatc gccgtcgcga gcgcttcgac catttgatat tgagactcgc
     1141 cgacgaactc gatgaggcgt ttcgcgagct gctcgtagtt cagcgtcccg gcgatggcgt
     1201 cgctggccgc ggcgacgcgc gcgtcggtgg cgatctcgag gtcgatgctg acgacctgct
     1261 tgatgcgccg ctcccactcc cagaagccga tgatcgtttc gatgcgcagc tcgcgcagga
     1321 agatcttgtc cactgtcgtc ctttatttat gggggcgtca gctcgcccaa cggccagcgc
     1381 ggtttcgcca cgatgcgcgg cagcccgttg cccgccgtcg ttcggcggca ccaccccgcg
     1441 taggcaatca tggcgccgtt gtccgtgcaa agcgccggcc gcggataata cacgcgcgcc
     1501 ccgacacgcc cgccgagctc cgcaaggctc gcgcgcagcg ctttattggc gccgacgccg
     1561 ccggcgatga cgagtctttg gagaccggtc gcctcgaggg cctgccggca cttgccggtc
     1621 agcgtgtcga cgacggcggc ctggaacgac gcagcaaggt cggcgcgctc gcgatccgcg
     1681 aggcggccgg cagagtcgcg gcacgagtcg atcgtcaggc gcacggccgt cttcaagccg
     1741 ctgaagctca tgtcgaggcc ctggttcttc atcggccgcg gcagcgagaa gcgctgcgaa
     1801 tcgccgcgct cggccaaggc cgcgagctgc gggccgcccg ggtacgggag gccgagcaaa
     1861 cgggccacct tgtcgaaagc ctcgccggcc gcgtcgtcga gcgtctcgcc gacgatgtgg
     1921 tagcgtccta cgccgctgac ctcgatcagc tgcgaatgac cgcccgagac gagcaaggcg
     1981 aggaacggga actccggcgc cgactcctcg agcatgcagg ccagcagatg cccttcgagg
     2041 tggtgcacgg ggatggcggg aatcttcagt ccataggcca acgacgacgc gagcgaaccc
     2101 ccgaccatca gcgcgccgat gaggccgggg ccggcggtat aggcgatggc gtcgaggtcc
     2161 cgaagcgtga ggccggcctc gccgagcacg tcttcgacca tcggcaatag cttcgcgatg
     2221 tggtcgcgcg acgcgagctc cggcacgacg ccgccatagg ccgcatggat cgcgatctgg
     2281 ctcgccagtc gctcggcgcg gattccggcg ccggattcgt agagggcgac gccggtctcg
     2341 tcgcagctgg tttcgacccc aaggacacgc ataaacaggc tttacagctt acgggacgcg
     2401 cggctaatat gccgctcttc agccggcttc gccggccaat ttttctgtac cgatcaacta
     2461 cggccattac ctaggatttc gatgccgggc gtcagaataa gagaaagcga gcacttcgat
     2521 gcagcgttgc gccgcttcaa gcgtgcttgt gaaaaagccg gtgtcctgac cgagctgcgt
     2581 cgccgagagt tctacgagaa accgactcag gaacgcaagc gcaagaaagc cgctgccgtg
     2641 aagcgccata tgaagcgcaa ctcgcgtacc cccaccacaa ggcgtcgccg actctactga
     2701 tcctctggcc tcgcggtcat gtcgctcaag gaccgtttaa ctcaagacat gcaggccgcc
     2761 atgaaaagcg gcgacaaggc acggttgagc gtgctgcgca tggcgcttgc cggtgtcaaa
     2821 cagcgcgaag tcgatactcg caggccgcta gacgacgccg gagtgcagac tgtcatcgag
     2881 cgcatgatca agcagggcaa ggacgcgctg cagcaattcc gcgacggcgg tcgcgcggac
     2941 ctcgtagcca aggaagaagg cgagctcaag gtgctccaga cctaccttcc gcaacagcta
     3001 tcggacgccg agctcgacac attcctcgcc gaatgcatcg acgccgccgg cgcgacgagc
     3061 gtcaaggatc tcggcaaggt catgggcgta atcaagacga gagcggcggg acgtgtcgac
     3121 atgagcaccg ttagcgcccg cgttcgtacg ctgctgacgc ccgagtaact cccgccgtcg
     3181 cggcggattc cgccgttgta gtcacgccga aggctcgcat aatggcgcgg catggccggc
     3241 cgaattcccc aagctttcat cgacgagctc gtcgcccgcg ctgacatagt cgaagtcgtc
     3301 ggcgcacgcg tcacgctgaa gcgcgccggc agcaactaca aaggcctctg cccgtttcac
     3361 ggcgagaaaa ccccgtcgtt cacggtgagc ccgtcgaagg gtttctatca ctgcttcggc
     3421 tgcagcgctc acggcacggc aatcggtttc ctgatggcgt acgacaacct cgagttcccg
     3481 caagccgtcg aagcgctcgc ggagatgatg gggctcgagg tgccgcgcga gaacgccggc
     3541 cagcccgagc acagggacga aaacgaggag ctgtacgcgc tgctgcgcga agcggatcag
     3601 atctatcgcg cggcgctgcg cagcagcgac actgcgatcg cctacttgaa gaagcgcggc
     3661 atcgacggcg cgacggccgg gcgcttcggt atcggctacg cgccgaacgc ctgggacacc
     3721 gtgctgcgcc agctcggcac gtcggacgct cgcgttgcaa agctgctgca agcggggctc
     3781 gtcgtcgcga acgagtcggg acgccgctac gaccgttttc gcgatcgcat catgtttccg
     3841 atccgcagcg cgcgcggcca cgtcattggc ttcggcggcc gcgtgctcga ctcgggcgag
     3901 ccgaagtatc tgaactcgcc tgagacgcct atcttccgca agggccaaga gctctacggg
     3961 ctctacgagg ctcggcagaa tcccggcaag ccgaagtcga tcatcgtcgt cgagggttac
     4021 ctcgacgtcg cgagcctcgt gcagttcggc gtcgagaacg tcgtcgcgac tctcggcacc
     4081 gcgatgacgg ccgaaaacat gaagcgcctt acacggctca cggaccgcgt gatcttttgc
     4141 ttcgacggcg accgcgcggg acgtgcggcc gcatggcgcg cgctcgagac cgcgttaccg
     4201 tacggcggcg gcacgttcga gctgaagttt ctattactgc ccgagagcga cgatcccgac
     4261 tcgttcgtgc gcagcaaagg gcccgatgcc tttcgcgcgt tggccgagac cgcggcgcca
     4321 ttgtcggact ttctcgtcaa ggagctgacg gcccgcgtgg atttgaattc cgtaga
//
LOCUS       DATLEE010000562         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_662437, whole genome shotgun sequence.
ACCESSION   DATLEE010000562 DATLEE010000000
VERSION     DATLEE010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662437"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VM692_12165"
     CDS             complement(<1..335)
                     /locus_tag="VM692_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HUQ52970.1"
                     /translation="MAAATQLPESTALAHVGLRLSDWFEKWFPDAFALALAVVAIVFI
                     ASLSVGSSLLDTAQWFGTGFWGLVAFTMQMSMIVVTGYAVATAPPVYRVVRLLASVPK
                     TGRGAAAYVG"
     gene            487..948
                     /locus_tag="VM692_12170"
     CDS             487..948
                     /locus_tag="VM692_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52971.1"
                     /translation="MGFLTWLEATGLAEWVRSSLPGYPSMIALHALGMAIMVGLSLLL
                     DLRLLGWFSGIPLAALQRFFGLAWVGFGINFISGSALFSAQATSYIVDWVFMTKMTLV
                     LLGAITAAILQPAVVKAGPSNEVSGGTKAVAGIAIVIWVVAIVMGRLTAYL"
     gene            961..1452
                     /locus_tag="VM692_12175"
     CDS             961..1452
                     /locus_tag="VM692_12175"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HUQ52972.1"
                     /translation="MDALQDWMVTTSISQAVLGYAWSWPTLESLHFLALCTLMGSLLI
                     MDLRLIGFQRIIPLQAVHSLMPVAIVSFAINLITGLGFLFGDPRTYTANWAFWAKMIL
                     IVLAGLNFLVYFTRVEPMILKLGPNEATPPQAKMVGVFSLLFWFGVLSFGRLLPYLGT
                     GGG"
     gene            1759..2571
                     /locus_tag="VM692_12180"
     CDS             1759..2571
                     /locus_tag="VM692_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52973.1"
                     /translation="MTDPATQAHAVVADEIVAALSPRLYRVTQFTTSDAAALGALSSR
                     AVTIVAVGDAAVRVAREALPAKPLVFCQVPDHASALKTGGPIWGVQTLPPLALQLKNW
                     RAVDPSLRTVALIVGTSQTDLADEARRAASALATDLLVETSASDRETLYLFRRVATSV
                     DGLWLLPDNEALSPQVLRELLSYASTRGIGVLTFNEALLSRGALLTAEAVPADVAATV
                     QHVVEQVVAGRTAGLPAMTPLSAATFSINSTVATSLGLPPTREQSWVVREPD"
     gene            2558..>2742
                     /locus_tag="VM692_12185"
     CDS             2558..>2742
                     /locus_tag="VM692_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52974.1"
                     /translation="MSPTEAAAPRERSRSLVQEVLGVQLAIAAIGGLIAVAGLAWTSG
                     TVVRDNLSHWAAQWASEL"
BASE COUNT          423 a          933 c          887 g          499 t
ORIGIN      
        1 ccgacgtagg ccgcggcgcc gcgccccgtc ttcggcaccg acgcgagcaa ccgcacgacg
       61 cgatacacgg gcggcgccgt cgcgacggca taacccgtca ccacgatcat cgacatctgc
      121 atcgtgaacg cgacgagccc ccagaacccc gtgccgaacc attgggcagt gtcgagcagg
      181 ctgctgccga cggagaggct tgcgatgaag acgatcgcga cgacggcgag cgccagcgcg
      241 aacgcgtcgg ggaaccactt ctcgaaccag tcggaaagcc gcaaaccaac gtgcgcgagt
      301 gctgtgcttt cgggcaactg cgtggcggcg gccattggcg ggcgactata gggggcgctg
      361 cgcgcgcttg gcaacaatgc aaaacgcccg catccgcctt gggtttctta agtggacagc
      421 cgcattgcct gcggctacgc tgccgccaga attattccta tagcgttttc ttagggggcg
      481 tcttccgtgg gattcttgac ttggctcgag gccaccgggc ttgccgagtg ggtgcgttcg
      541 tcgcttccgg gctacccgtc gatgatcgcg ctgcatgccc tcggcatggc aatcatggtc
      601 ggcctatcgc tgctgctcga cctgcgtctg ctcggttggt tcagcggcat cccgctggcc
      661 gcgctgcagc gcttcttcgg cctcgcgtgg gtcggcttcg gcatcaactt catctcgggc
      721 tcggcgctgt tctcggcgca ggcaacgagc tacatcgtcg actgggtgtt catgactaaa
      781 atgacgctcg tgctgctcgg cgcgatcaca gctgcgatac tacagcctgc cgtcgtcaaa
      841 gcagggcctt cgaacgaggt ctcgggcggc acgaaagccg tcgcggggat cgccatcgtg
      901 atttgggtcg tcgcgatcgt catgggacgc ctcacggcgt atctatagga ggatctgaac
      961 atggacgcgt tacaggattg gatggtcacg acgtcgatca gccaggccgt gctcggctac
     1021 gcctggagct ggccgacgct cgagtcgctg cacttcctcg cgttgtgcac gctgatgggc
     1081 tcgttgctga tcatggacct gcgcctgatc ggttttcagc gaatcattcc gctacaggcc
     1141 gtgcatagcc tgatgccggt cgcgatcgtc tcgttcgcga tcaacctgat cacgggcctg
     1201 gggttcttgt tcggcgaccc gcggacctac acggcgaact gggcgttctg ggccaagatg
     1261 atcctgatcg tgctcgccgg cctcaatttc ctcgtctatt tcacgcgagt cgagccgatg
     1321 atcttgaagc tcggccccaa cgaggcgacg ccgccgcagg ccaagatggt cggcgtgttc
     1381 tcgctgctct tctggttcgg cgtgctctcg ttcggccgct tactgccgta cttgggaaca
     1441 ggcggcggct aaacgctccg caccgcggcg cgccgggcgt cgccttattt gtgaagccca
     1501 acacgccgtg ttggccggcc tcccccgcac actggcagtt ccatgcggaa cgcctaccga
     1561 cgacggtctg gactgccgcc ggcggcggtc atgctctcgc tgcttgcggg ctgcgcggcc
     1621 ccaggcccgc cccccgttga gccggcgctc gagcccatcg tcgtagaggc gtcgccgcta
     1681 ccggtaccca cgcgcaccgt cgaacccatt cgcccaacac gcccggcgcc gcagcgggcc
     1741 ctcgccgaga tcgcggtcgt gaccgacccc gccacgcagg cgcacgcggt cgtcgccgac
     1801 gaaatcgtgg cggccctgtc gccgcggctc tatcgcgtga cgcagttcac gacgtcggac
     1861 gccgccgccc ttggcgcgct gagcagccga gccgtcacca tcgtggccgt cggcgacgcc
     1921 gccgttcgcg ttgcgcgcga agcactcccc gcaaaaccac tcgtattctg tcaggtgccg
     1981 gatcacgcct cggcactcaa aacgggggga ccgatctggg gcgtgcaaac gctgccgccg
     2041 ctggctctgc agctcaagaa ctggcgagcc gtcgatccgt cgctgcggac cgttgccttg
     2101 atcgtcggca catcccaaac cgacctcgcc gacgaagcac ggcgcgctgc gagcgcactc
     2161 gcaacggacc tgctcgtcga aacgtcggcg tcagatcgcg aaacgctgta cctgtttcgc
     2221 cgcgtcgcaa cctcggtcga cggcctctgg ttgttgccgg acaacgaggc gttgagccca
     2281 caggtgctgc gcgagctgct gagctatgcg tcgactcgcg gcattggcgt gttgacgttc
     2341 aacgaggcgc tgttgagccg cggcgcactg ctgactgccg aggccgtgcc ggccgacgtg
     2401 gccgctaccg tgcagcacgt ggtcgagcaa gtcgttgccg gccgcacggc gggattgccg
     2461 gcgatgacac cgttgtcggc cgcgacgttc tcgatcaact cgaccgttgc aaccagcctc
     2521 ggtctgccgc ccacacgcga gcaaagctgg gtggttcgtg agcccgactg aggcggcagc
     2581 accgcgcgaa cgcagccgca gcctcgtgca agaggtgctc ggcgtgcagc tcgcgatcgc
     2641 tgcgatcggc ggcttgatcg ccgtcgcggg cctcgcatgg acctcgggta ccgtcgtccg
     2701 cgacaacctc tcgcactggg cggcgcaatg ggcctcggag ct
//
LOCUS       DATLEE010000563         5048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7776312, whole genome shotgun sequence.
ACCESSION   DATLEE010000563 DATLEE010000000
VERSION     DATLEE010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7776312"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VM692_12190"
     CDS             complement(<1..426)
                     /locus_tag="VM692_12190"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HUQ52975.1"
                     /translation="MQWFGKGIGGLIGAAIAGPVGSLLGALVGHQFDQSLGGRRGSVR
                     EISRLFFETTFEVMGQVAKIDGRVSEDEVRVARRIMQGMRLTEEQVQSAIEYFTRGKR
                     ADYPLEARLAALAEQLGGRGDLARAFVQIQLQSAIGAGNV"
     gene            534..1430
                     /locus_tag="VM692_12195"
     CDS             534..1430
                     /locus_tag="VM692_12195"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HUQ52976.1"
                     /translation="MAESLFESNLVSLKLTARGKVRDIYNVDAERLLIVATDRLSAFD
                     VVLPDPIPGKGVVLTSISNFWFKKLETVVPNHLTGIAPEDVLSNPADHATVKNRAVVV
                     RKLKPLPIEAVVRGYLIGSGWKDYCKSGRVSGVDLPPGLELAQRLREPIFTPSTKAAA
                     GLHDENISMEQVKSLIGPELTNQVREASLQLYTRATEYARQRGIIIADTKFEFGLDAS
                     GSLRVMDEVLTPDSSRFWPADTYRAGSSPPSFDKQYVRDYLETLDWNKQAPGPKLPRV
                     VIERTRAKYVEALQRLTSDKST"
     gene            1463..2785
                     /locus_tag="VM692_12200"
     CDS             1463..2785
                     /locus_tag="VM692_12200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HUQ52977.1"
                     /translation="MEFITRPIERRLWPRGKRTEPVPRAIIVAQFVYALVRDFMHGDL
                     SMRAMSLVYTTMIAIVPLLAFVFSFFKGLGLHRDLEPILQGFLAPLGPRASELSSQVI
                     GFVDNVSGFALGAISIAILLYSALSMAQKVESSFNYVWRVDRPRSFARRFAEYLSVML
                     VGPLIMLAAMGFTATLSSTAAMTRLREIGVIGDMFEALSWFTPYALIIASFSSLYVFV
                     PNTRVQLLPAAIGGVFAGILWAGGGSTFASFVVSVSRYEPIYAGFAIVIVAMIWLYLS
                     WLILLLGAQLAFYVQHPEYLPLGQRTLTASNRTRERLALSTMLLVGRDFEKPGHGWRI
                     ESAAAQLRVPRHLLEPVAGALMDAGLLTRTNENRLIPARDLRRIGVVAILAAVRSERD
                     SHHQGDDDWTPTVAAVADDVEAAIRSTLGERTLADLVDADSRSTATPS"
     gene            complement(2790..4124)
                     /gene="pmbA"
                     /locus_tag="VM692_12205"
     CDS             complement(2790..4124)
                     /gene="pmbA"
                     /locus_tag="VM692_12205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HUQ52978.1"
                     /translation="MDQHAVGESLQAVAAQAVALARKFGSDQAEVGVSYEEGLSVSVR
                     MGELESVERQRDRGLAITVYRNQCKGSASTTDFSATSIEETVRKAISIGSFTTADEYA
                     GLADAELMAKNPQDLELYFPWGVDVDQATDLALRSENAARSLDPRIANSEGASVSSGV
                     GQRVYANSHGFVGSYPTSTHSMSCSVLAKAGESLERDYWYTVSRHPDDMESPEEVGQE
                     AARRAVQRLEARTLSTRKVPVLYPAELAKGLFGHLIAAVRGTAQYRRASFLLDAAGKP
                     VLPSFIDIEEDPWIPRALASAPFDAEGVQTRRRKIVEQGVLQGYVLSSYSARRLGLTT
                     TGNAGGVHNLLVKPTAGTLEDLIKDCDEAFVVGELLGQGVNTVTGDYSRGAAGFWVER
                     GAIVHPVHEVTIAGNLKDLFREIQAVGSDIDSRGTVRCGAVRVDGLTLAGGG"
     gene            4156..4644
                     /gene="purE"
                     /locus_tag="VM692_12210"
     CDS             4156..4644
                     /gene="purE"
                     /locus_tag="VM692_12210"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HUQ52979.1"
                     /translation="MKPLVGVIMGSRSDWSTMLHATETLTALGVTFETRVVSAHRTPD
                     LLYEYAGSAVARGLEVLIAGAGGAAHLPGMTAAKTRLPVIGVPVRSRALQGIDSLLSI
                     VQMPRGIPVATVAIGKAGAVNAALLAAAIVARTDEGVRERLARYRAEQTESVLANPDP
                     AK"
     gene            4698..>5048
                     /locus_tag="VM692_12215"
     CDS             4698..>5048
                     /locus_tag="VM692_12215"
                     /EC_number="4.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716533.1"
                     /note="With PurE catalyzes the conversion of
                     aminoimidazole ribonucleotide to carboxyaminoimidazole
                     ribonucleotide in the de novo purine nucleotide
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HUQ52980.1"
                     /translation="MLALAGIPLGLSFRFLDPASDAPAGEVGELIVGVYDDPELLDRL
                     ADGAEAVTYEFENVPVEAARRVGAVPGAPALEASQDRLVEKQLFRRLGIPTPRIDSEV
                     DTFPALLKTRRLGYD"
BASE COUNT          796 a         1738 c         1630 g          884 t
ORIGIN      
        1 gacgttgccg gccccgatcg cggactggag ctgaatctgc acgaacgcac gcgccaagtc
       61 gccgcgcccg ccgagctgct ccgcaagcgc cgcgagcctc gcctctagcg gataatcggc
      121 gcgcttgccg cgcgtgaagt attcgatcgc gctttgcacc tgctcttccg tcaggcgcat
      181 gccctgcatg atgcggcgcg cgacgcgcac ctcgtcctcg gagacgcggc cgtcgatctt
      241 ggcgacctgc cccatgactt cgaacgtcgt ctcgaagaac agccgactga tttcgcgtac
      301 cgacccgcgg cggccgccga ggctctgatc gaattggtga ccgacgagcg cgccgagcag
      361 cgagccgacg ggtccggcaa tcgccgctcc gatcaaccca ccgatcccct tgccgaacca
      421 ctgcatcgga ggctccgcaa tctcgctgtc aggcgccgga gtcaagttgc cgaacggcgc
      481 tacgagggct cattatcgtg gctttgaacg cctaagcaac gagacccatc gccgtggccg
      541 agtcgctttt cgagtccaac ctcgtgagct tgaagctcac cgcgcgcggc aaggtccgcg
      601 acatctacaa cgtcgacgcc gaacggctgc tgatcgtcgc gaccgatcgg ctctcggcat
      661 tcgacgtcgt gctgcccgat ccgattcccg ggaagggcgt cgttctgacg tcgatctcga
      721 acttctggtt caagaagctc gagaccgtcg tgccgaatca cctgaccggc atcgcacccg
      781 aagacgtgct gtcgaatcct gcggatcacg cgacggtcaa gaatcgcgcc gtcgtcgtgc
      841 gcaagctgaa gccgctgccg atcgaggccg tcgtgcgcgg ctatctgatc gggtcgggtt
      901 ggaaggacta ctgcaagtcc ggccgcgttt cgggcgtcga cctgccgccc ggcctcgagc
      961 tcgcgcagcg gctgcgcgag ccgatcttca cgccgagcac gaaagcagcg gcaggcctgc
     1021 acgacgagaa catctcgatg gaacaggtca agagcttgat cggccccgag ctgacgaacc
     1081 aagtccgcga ggcttcgctg cagctctaca cacgcgcgac agagtacgcg cggcagcgcg
     1141 gcatcatcat tgcggacacc aagttcgagt ttggtctcga cgcgagcggc tcgctgcgcg
     1201 tcatggacga ggtgctgacg ccggactcgt cgcgcttctg gcccgcggac acctaccgcg
     1261 ccgggtcctc gccgccgagc ttcgacaagc agtacgtgcg cgactacttg gagacgctcg
     1321 attggaacaa gcaggcgccc ggccctaagt tgccgcgcgt cgtcatcgag cgcacccgcg
     1381 ccaaatacgt cgaagccctg caacggctca cgagcgacaa gtcgacatga cgtcggctcg
     1441 ttcgaggcga gcggtccgct gaatggagtt catcactcgg ccgatcgagc gtcgtctctg
     1501 gccgaggggc aagcgcacgg aacccgtgcc gcgcgcgatc atcgtcgctc agttcgtcta
     1561 cgcgctcgtc cgcgatttca tgcacggcga cttgagcatg cgtgcgatga gcctcgtcta
     1621 cacgacgatg atcgccatcg tgcccctgct cgcgttcgtg ttctcgtttt tcaaagggct
     1681 tgggctgcat cgcgatctcg agccgattct gcaaggattc ctcgcgccgc tcgggccgcg
     1741 cgcgtctgag ctgtcgagcc aggtgatcgg cttcgtcgac aacgtcagcg gcttcgcgct
     1801 cggcgccatc agcatcgcga tcctgctgta cagcgccctg tcgatggcgc aaaaagtcga
     1861 gagcagcttc aactacgtct ggcgcgtcga tcgcccgcgc agctttgcac gccgcttcgc
     1921 cgaatacttg agcgtcatgc tcgtcggccc gttgatcatg ctggccgcga tgggcttcac
     1981 ggccacgttg tctagcaccg cggcgatgac gcgcctgcgc gaaatcggcg tcatcggcga
     2041 catgttcgag gcgttgagct ggttcacgcc gtatgcgctg atcatcgcga gcttctcgtc
     2101 cctctatgtg ttcgtgccga acacgcgcgt gcaactcttg cccgcggcca tcggcggcgt
     2161 gttcgccggc atcctgtggg ccggtggcgg cagcacgttc gcgagcttcg tcgtttccgt
     2221 cagccggtac gagccgattt acgcgggttt cgcgatcgtg atcgtcgcga tgatctggct
     2281 gtatctgtcg tggctgatcc tgctgctcgg tgcccagctc gcgttctacg tgcagcatcc
     2341 cgagtatctg ccgctcggcc aacgaacgct gacggcgtcg aaccgcacgc gcgagcggct
     2401 cgcgttgtcg acgatgctgc tcgtcggccg cgatttcgag aagccgggcc atggctggcg
     2461 catcgaaagc gccgcggcgc agctgcgcgt gccgcgtcac ttgctcgagc ccgtggcggg
     2521 cgcattgatg gatgcggggc tattgacgcg gacgaacgag aaccgcttga tccccgcgcg
     2581 cgacttgcgg cgcatcggcg tcgtcgccat cttggccgcg gtgcgcagcg agcgcgactc
     2641 gcatcatcaa ggcgacgacg actggacccc gacggtcgcc gccgtggccg acgacgtcga
     2701 ggcggcgatc cgcagcacgc tcggcgagcg caccctcgcc gacctcgtcg acgccgactc
     2761 gcgctcgacg gccacgccga gctgagagct cagcctccgc cggccaacgt caaaccgtcg
     2821 acgcgaacgg cgccgcaccg caccgtgccg cgcgaatcga tgtcggagcc gacggcctgg
     2881 atctcgcgga acaagtcctt gaggttgccg gcaatcgtga cctcgtggac ggggtgcacg
     2941 atcgcgccgc gctcgaccca gaatccggcg gcgccgcgcg agtaatcgcc cgtcacggtg
     3001 ttgacgccct ggccgagcag ctcgccgacg acgaacgcct cgtcgcagtc cttgatgaga
     3061 tcctcgagcg tgccggccgt cggcttgacg agcaggttgt gcactccgcc ggcattgccg
     3121 gtcgtcgtga gcccgagccg gcgcgccgag tagctcgaca gcacgtagcc ttgcagcacg
     3181 ccctgctcga cgatcttgcg ccgccgcgtc tgcacgcctt cggcgtcgaa cggcgcgctc
     3241 gcgagcgcgc gcggaatcca cgggtcctcc tcgatatcga tgaacgacgg caaaaccggc
     3301 ttgccggccg cgtcgagaag gaacgacgcg cggcgatact gcgcggtgcc gcgcacggcc
     3361 gcgatcaagt gaccgaacaa gcccttcgcg agctcggccg gatagagcac gggcaccttg
     3421 cgcgtcgaca acgttcgcgc ttcgagccgc tgcacggcgc ggcgcgccgc ctcctgtccg
     3481 acctcctccg gcgactccat gtcgtcgggg tgccggctta ccgtgtacca gtaatcgcgc
     3541 tccagcgact ccccggcctt tgcgagcacg ctacagctca tcgagtgcgt gctcgtcgga
     3601 taggagccga cgaagccgtg cgagttcgca taaacgcgct ggccgacgcc gctcgacacc
     3661 gatgcgccct cggagttcgc gatgcggggg tcgagcgacc gcgccgcgtt ctcgctgcgc
     3721 agcgcgaggt ccgtcgcctg atcgacgtcg accccccacg gaaaatacag ctctagatcc
     3781 tgcggattct tcgccatgag ctcggcgtcc gcgaggccgg catattcgtc ggccgtcgta
     3841 aagctaccga tgctgatcgc cttgcgaacg gtctcttcga tgctcgtcgc cgaaaagtcc
     3901 gtcgtgctcg cgctgccctt gcactgattg cgataaaccg tgatcgcgag cccgcggtcg
     3961 cgttgccgct cgacggactc gagctcgccc atgcgcacgc tcacggacaa tccttcctcg
     4021 tagctgacgc cgacctcggc ttgatcggag ccgaacttgc gcgcgagtgc gacagcctgc
     4081 gctgcgacgg cctgcagcga ttctccaact gcgtgttgat ccattgccac attctaactg
     4141 ctgtacaatg cgccgatgaa accattggtc ggcgtcatca tggggtcccg ctcggactgg
     4201 tccacgatgt tgcacgccac cgaaactctg acggcgctcg gcgttacatt cgaaacgcgc
     4261 gtcgtttccg ctcaccgcac tccggatctg ctctatgaat acgcgggcag cgccgtcgct
     4321 cgcgggctcg aggttctgat tgccggggcc ggcggtgccg cgcacttgcc cggcatgacg
     4381 gctgcgaaga cgcgcttgcc cgtcatcggc gtaccggtcc gctcgcgcgc gctgcagggc
     4441 atcgactcgc tgctgtcgat cgtgcagatg ccgcgcggca ttcccgtcgc caccgtagcg
     4501 atcggcaaag ccggcgccgt caacgcggcg ctgctggcgg cggcgatcgt ggcgcgcacg
     4561 gacgagggcg tccgcgagcg cctcgcccgg taccgagcgg agcagacaga gtcggtgctg
     4621 gcgaaccccg acccggccaa gtaacagtct gttacttggc gacggtcggc gtcatcggag
     4681 gaggtcagct cgggcggatg ctcgcgctcg cggggatccc gctcgggctc tcgttccgct
     4741 tcctcgatcc ggcctccgac gcgccggccg gtgaagtggg ggagctgatc gtgggcgtct
     4801 acgacgatcc tgagctgctc gaccggcttg ccgacggggc cgaggcggtt acgtacgagt
     4861 tcgagaacgt cccggtcgag gctgcgcgtc gtgttggtgc ggtcccgggg gcccccgcgc
     4921 tcgaggcttc gcaggaccgg ctcgtcgaga agcagctgtt ccgccgcctc gggatcccga
     4981 cgccccggat cgacagcgag gtggacacgt ttccggcgct cctgaagaca agacgcctcg
     5041 gctatgac
//
LOCUS       DATLEE010000564         6937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5868604, whole genome shotgun sequence.
ACCESSION   DATLEE010000564 DATLEE010000000
VERSION     DATLEE010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5868604"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VM692_12220"
     CDS             <1..192
                     /locus_tag="VM692_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HUQ52981.1"
                     /translation="GMSGYDVALQLRERTREWGTGIRLIAITGYGATADRARAADVGF
                     SAHLVKPVDPEVLKPLLAS"
     gene            291..2492
                     /locus_tag="VM692_12225"
     CDS             291..2492
                     /locus_tag="VM692_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ52982.1"
                     /translation="MTDETTKNKGLRAASSPEAAEWEDTRARLAAIVDSSEDAIVSKT
                     LEGIVTSWNAGAERVFGWTAEEMIGQPILKLIPTELQHEEGEILAKLRAGERIERYET
                     VRQHKNGSRLDISLTVSPIRDSRGVVSGAAKIAHDITARRQAERQAQEEARALEMLNR
                     VGRAVAAQLDLERIVQIVTDAATDVSHAAFGAFFYNVTERGKESYWLYALSGAPRAAF
                     ANFPMPRATEVFAPTFHGSGAVRSDDIRKDARYGKMAPHFGMPEGHLPVCSYLAAPVV
                     SHTGTVLGGLFFGHPDAGVFTERAERLVVALASQAAIAIDNAKLIQELREREAELSKV
                     AAERAQLLDSERAARTEAERLSRIKDEFLATLSHELRTPLHAIQGWTEILMQSPRSDQ
                     DKQALQTIDRNVRAQVRIISDLLDMSRIVAGKLHLEVQPLYLHETVEQALDSVRQSAD
                     AKHLRINTLLDSEIGLVRGDANRLQQVFWNLFSNAVKFTPAGGRISVVLERVNSHVEI
                     VVEDTGIGIRSDFLPYVFDRFRQADPALTRRHSGLGLGLSIVKNLVEMHGGSVRVKSP
                     GENQGSTFIVALPVSHVRATDQHPPTTTVDRLDSIELPRLTDVRVLVVDDEGDGRELI
                     ARILEDCGARPVCASNAPQALECLSRQDFDVLLSDIGMPDVDGYELIRRVRAREGSAR
                     DGRLAAVAITAYARPEDRQRSLLAGYQMHLAKPIEARELIAGIASLLHLSR"
     gene            2575..4476
                     /locus_tag="VM692_12230"
     CDS             2575..4476
                     /locus_tag="VM692_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HUQ52983.1"
                     /translation="MLKRLAAVSLAVTLTASAQAQDPAAQPASPLDNRVVMTGEQVVR
                     ILDETADWYRMLGVQQQSATQPSDLLILYANRQSADRVVALAFELARANAELLSSQAE
                     LQQQADAASSPPALQRELSELDSRKDALEAEIATTRRELTGAAADERNMLQARVAELQ
                     SELDLVSARRNLVNNMSELAHETDANGSGVAALKERINVIEMSLPATSGLMPELGGAQ
                     EGAAAVANTRVADQLGARRFGIWDLAANVLRLQNKLSTIDDVDRRTEQLQQTFTQIRA
                     MPEAQIAMLSARGDELGARPAGTNPAEIRAARGEYDTIAWLFDQTSSILVPLTKAGVL
                     LEQYRNNLDSWRVATRAQFFSALKTLAARVLLLGVLLAAVFAGGEVWKRAVFNYVQET
                     RRRHQLLLVRRIVVWAVALIIVAIAFATEASSFATFAGLLSAGVAVAMQSVLVSIVGY
                     FFLIGKYGLRVGDHIQIGAVSGEVIDLGLVRLHLMELGPQYAPTGRVVALPNSVVFQV
                     TGGIFKQIPGVSLAWRELSFTLPADADYAAIKERLLAAAARVLERYREELERQTRELH
                     KTSSGGIGEQAQAQVQLRFSANSIEAIVRYPVPLQRAGEVEERMSRRLLEAVRGGTDG
                     RAQLSTQPA"
     gene            complement(4597..5784)
                     /locus_tag="VM692_12235"
     CDS             complement(4597..5784)
                     /locus_tag="VM692_12235"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HUQ52984.1"
                     /translation="MRIINQSVLTLASAALFCGVALGQDASSAHSSSETQLKSGDVRA
                     THLIGAMVKRLTGDSVGEVKDLIVSADKDVRLAVISVGGVLGVGSKTIALPFNQFQVA
                     PDGATLFLTLSEEELSARPAFDLENVTADSPSTDNDFAAPAQQTLPDASSPGFVPSNT
                     THESFAQDTGQPAEAESAQPSFGSAQPSSFEAAQPSFEAAESSQPSAFESQPAAPEIV
                     VPAEQVQVTADAQVVEEAQLAERQAHEAEQAELAEQAQPEQQAQEAELARQAEQAHEA
                     ALAQLSEQAQLDQQARQAQAASKAKASNQAASALIGKDVVDPQNTAIGKVNDVVVTAE
                     PPQVQVIVQLSEELGSGAKLVAVPLPELTISQGGSEDARQIDRVETELTVAQLEALPQ
                     FQY"
     gene            6007..>6937
                     /locus_tag="VM692_12240"
     CDS             6007..>6937
                     /locus_tag="VM692_12240"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HUQ52985.1"
                     /translation="MRIRLTCIAAAVASLANSGESAAQQQGLDTIVVTAERIQADLEA
                     ERAVTPGAVTNLDGAKFYERRVSQLSDMLRYVPGVYAESYNGNDDVFYSSRGSNLDAT
                     DYDKNGVKFLQDGLPVTAADGNNHNRALDPLGARYATVAHGANALAYGASTLGGAVDF
                     TTPTAHSTMPSVATSAGSFGEWNLRATMGAVFSDELDGLITAEVQQRDGYREHSAHDR
                     KGLYANLGWSVSENVSTRFYATHIDYDSQLPRELTPAQYAQDPNQARADAILGNHNKQ
                     VAASRLAFKTTFAEVADGTLELGVSYENQELYHP"
BASE COUNT         1204 a         2264 c         2280 g         1189 t
ORIGIN      
        1 ggcatgagcg ggtatgacgt ggcgctgcag ttgcgcgaac gaacacgaga gtggggcacg
       61 ggtatccgtc tcatcgcaat cacgggctac ggggctacgg cggatcgcgc ccgtgccgcc
      121 gatgtgggct tctcggcgca cttggtgaag cccgtggacc cagaggtttt gaagccgctg
      181 ctggcttcgt agccggttcg gcgcggcgcg ccgtttcgcg ttgtagtcgg cacgaaaggt
      241 gcagcgctct atcttaatgc gcggaagact gcgcagtgga gactgtgcag atgaccgatg
      301 aaacgacgaa gaacaagggc ttgcgcgccg cgagtagccc ggaagcggcg gaatgggagg
      361 acacccgagc ccggctcgcc gctatcgtcg attcatccga ggacgccatc gtcagcaaga
      421 cgctcgaggg catcgtgacc tcgtggaacg ccggtgccga gcgagtcttc ggctggaccg
      481 ccgaggaaat gatcggtcag ccgatcctca agctgattcc aaccgagctg caacacgaag
      541 agggtgaaat cctcgccaag ctgcgcgccg gcgagcggat tgagcgctac gagacggtcc
      601 gccaacacaa gaacgggagt cgtctcgaca tttcgctgac ggtgtcgccg attcgcgata
      661 gccggggcgt cgtctcgggc gcggcgaaaa tcgcgcacga catcacggcc cggcggcagg
      721 ccgagcgtca ggcgcaagaa gaggcgcgag cgctcgaaat gctcaatcgc gtcggccgag
      781 ccgttgccgc gcaattggac ctggagcgca tcgtgcaaat cgtcacggat gccgcgaccg
      841 acgtctcgca tgcggccttc ggcgcgttct tctacaacgt cacggagcga gggaaggagt
      901 cttactggct gtacgcgctg tcgggcgcgc cgcgtgcggc gttcgcaaat ttcccgatgc
      961 ctcgcgcgac cgaagtgttt gccccgacgt ttcacggcag cggtgccgtg cgctccgacg
     1021 acattcgcaa ggatgcgcgc tacggcaaga tggcgccgca cttcggcatg cccgaagggc
     1081 acctaccggt ctgtagctac ctcgcagcgc cggtggtgtc gcatacgggc accgtgctcg
     1141 gcgggctctt ttttggccat cccgacgcgg gagtgttcac cgagcgtgcg gagcgtctcg
     1201 tggtcgcact cgcctcgcag gcggcgatcg cgatcgacaa tgccaagctc attcaggagc
     1261 tgcgcgagcg cgaggccgag ctgagcaagg tggcggccga gcgcgcacaa cttctcgact
     1321 cggagcgagc cgcgcgcacg gaggccgagc gcctcagtcg catcaaagac gagttcctcg
     1381 ccacgctatc gcacgagctg cgaacgccgt tacatgctat tcaaggctgg accgaaatct
     1441 tgatgcagag tccgcgctcc gaccaagaca agcaggcgtt acagacgatc gatcgcaacg
     1501 ttcgagcaca ggtgcgcatc atcagcgatc tgctcgacat gagccgcatc gtggcgggga
     1561 agctccacct cgaggttcaa ccgctctatt tgcacgagac ggtcgagcaa gcgctcgatt
     1621 ccgtgcgtca atccgccgac gccaagcacc ttcggatcaa cacgctgttg gattccgaaa
     1681 tcggcctcgt acgcggcgat gcgaaccgtt tgcagcaagt gttctggaac ttgttttcga
     1741 acgcggtgaa gttcacgccg gcgggcggcc gtatctccgt ggtactcgag cgcgtgaact
     1801 cgcacgtcga gatcgtcgtc gaagacaccg gcatcggcat ccgcagcgac ttcctgccgt
     1861 acgtcttcga tcggtttcgc caagcggatc ccgcgttgac gcgccgccat agcggcctcg
     1921 ggctcggact gtcgatcgtc aagaatttgg tcgagatgca cggcggatcc gttcgcgtga
     1981 agagccccgg tgagaaccag gggagcacgt tcatagtcgc gctcccggtg tcccacgttc
     2041 gggcgaccga tcaacacccg ccgacgacga ccgtcgatcg cttggattcg atcgagttgc
     2101 cgcgtctgac cgacgtacgc gtgctggtgg tcgacgacga gggtgacggt cgcgagctga
     2161 tcgcgcgcat tctcgaggac tgcggtgctc ggccggtgtg tgcatcgaat gcgccgcagg
     2221 ccctcgagtg cctctcacgc caagatttcg acgttctctt gagcgacatc ggcatgcccg
     2281 acgtcgatgg ctacgagctg atacgtcggg tacgggcacg cgaaggttcc gcccgcgacg
     2341 ggcggctcgc cgccgttgcg atcacggcct acgctcggcc cgaggaccgg cagcgctcgc
     2401 tgttggccgg ttatcaaatg catctcgcca agccgatcga ggcgcgcgag ctcatcgccg
     2461 gcatcgccag cttgctgcac ttgagccgct gagcgccgcc ctggcgcagt cggtggcgac
     2521 cgggcatgcg gttagcaggt accattgctc gctcacatca agcgctacga agcaatgctg
     2581 aagagactcg ctgccgtatc tctcgccgtg acgttgacgg cctccgctca agcgcaggat
     2641 cccgccgcgc agcccgcgtc gccgctcgac aatcgggtgg taatgacggg cgagcaggtc
     2701 gttcgcatcc tcgacgagac ggcggactgg tatcgcatgc tcggcgtgca gcagcaatcg
     2761 gccacgcagc ccagcgactt gctgattctc tacgccaacc ggcagtcggc ggaccgcgtc
     2821 gtcgcgctcg cgttcgagct cgcgcgggcg aacgccgagc tcttgagcag tcaggcggag
     2881 ctgcagcagc aggcggacgc cgccagctcg ccaccggcgc tacagcgaga gctcagcgag
     2941 ctcgattctc gcaaggacgc gctcgaggcg gagatcgcga cgacgcggcg cgagctcact
     3001 ggggcggcgg ccgacgagcg caacatgctg caagcgcgcg tcgcggagct acaaagcgag
     3061 ctcgacctcg tcagtgcgcg ccgcaatctc gtgaacaaca tgagcgagct cgcgcacgag
     3121 acggatgcca acggctcggg cgtggccgct ctgaaggagc ggatcaacgt catcgagatg
     3181 tcgcttcccg caacctcagg gctcatgccg gagctcggtg gggcgcaaga gggcgctgcc
     3241 gcagttgcga acacgcgggt ggcagaccag ctcggggctc gacggttcgg catttgggat
     3301 ctcgctgcca acgtgctgag gctgcagaac aagctttcga cgatcgacga cgtcgaccgc
     3361 cgcacggagc agctgcagca gacgttcacg cagatccgtg caatgccgga ggcgcagatc
     3421 gcgatgcttt cggcacgcgg cgacgagctc ggggcgcggc cggcgggaac caatccggcc
     3481 gagatcagag ccgcgcgcgg cgagtacgac acgattgcgt ggctcttcga ccaaacgtcc
     3541 tcgattctcg tcccgctgac gaaggccggc gtgctcctcg agcagtaccg caacaatctc
     3601 gacagctggc gcgtagccac gcgagctcag ttcttctcgg cgctcaagac gctggcggcg
     3661 cgcgtgctgc tactgggcgt gctgctcgcg gccgtgttcg cgggcggcga agtttggaag
     3721 cgggcagtgt tcaactacgt tcaggagacg cgccggcggc atcagctgct gctcgtgcgc
     3781 cggatcgtgg tctgggccgt cgcgctcatc atcgtcgcca tcgcattcgc cacggaggcg
     3841 agctcgttcg cgacgttcgc cggcttgctg agcgccggcg tcgcggtcgc gatgcagagc
     3901 gtgctcgtgt cgatcgtcgg ctactttttc ctgatcggca aatacggcct gcgagtcggc
     3961 gatcacattc agatcggcgc ggtctccggc gaagtcatcg atctcggcct cgtacgtttg
     4021 cacctcatgg agctcggccc gcaatacgct ccgacgggcc gcgtcgtggc gctgccgaac
     4081 tccgtggtgt tccaggtgac tggaggcatc ttcaagcaga tccccggcgt cagcctcgcc
     4141 tggcgcgagc tgagcttcac tttgccggcc gacgccgact acgcggcgat caaggagcgg
     4201 ctgctagcgg ctgcagctcg cgtgctcgag cgctaccgcg aggagctcga gcgccaaacg
     4261 cgcgagctgc acaagacgtc gtcgggcggc atcggcgaac aagcacaagc gcaagtgcaa
     4321 ctgcggttct cggccaactc gatcgaggcg atcgttcgct accccgtgcc gctgcagcgg
     4381 gcgggcgaag tcgaagagcg tatgtcgcgc aggctgctcg aggccgtccg cggcggcacg
     4441 gacgggcggg cgcagctcag cacgcagccg gcgtagcaag caactccgcg tacaagcgtg
     4501 ctgcgatagc ctcgtcagct cgccactggc gcgcgcgcgt gctcaggcaa ggcggggggt
     4561 tagagcacgc gcgcgactag cgactaaatt ttgatatcaa tattgaaatt gcggcaaagc
     4621 ctctagctga gcgacggtaa gctcggtttc gacccgatcg atctggcgcg cgtcctcgct
     4681 gccgccttgg ctgatcgtca gctcgggaag cggcacggcc acgagcttcg cgccgctgcc
     4741 gagctcctcg ctaagctgaa caatgacttg cacttgcggc ggctccgccg tcacgacgac
     4801 gtcgttgacc ttgccgatcg cggtgttctg agggtcgacc acgtccttgc cgatcagagc
     4861 gctcgccgct tggttcgacg ccttcgcctt gctcgccgct tgcgcttgcc gcgcttgctg
     4921 gtccagctgc gcctgctccg acagctgggc cagcgccgcc tcgtgcgctt gctctgcctg
     4981 ccgcgccagc tcggcctctt gggcttgctg ctccggctga gcttgctcgg cgagctccgc
     5041 ttgctcggcc tcgtgcgcct gccgctcggc cagttgcgcc tcttctacca cctgcgcgtc
     5101 cgctgtcacc tgcacttgct ctgccggcac gacgatctca ggcgccgcgg gctgcgactc
     5161 gaacgccgac ggctgcgacg actccgccgc ctcgaaagac ggctgcgccg cctcgaacga
     5221 cgacggctgc gccgacccaa acgacggctg cgccgactca gcctcggcag gctggccggt
     5281 gtcctgcgca aagctctcgt gcgtcgtgtt gctgggcacg aagcccgggc tgcttgcgtc
     5341 cgggagcgtc tgttgcgccg gcgccgcgaa gtcgttgtcc gtcgacgggg aatcggccgt
     5401 gacgttttcg agatcgaagg cgggccgcgc cgagagctcc tcttccgaca gcgtcagaaa
     5461 gagcgtggcg ccatcgggcg caacttggaa ctgattgaac ggcaacgcga tcgttttgct
     5521 gccaacaccg aggacgccgc ccacggagat cacggcgagt cgaacgtctt tgtcggcgga
     5581 gacgatcaaa tccttgacct cgccgaccga gtcgcccgtc aggcgcttga ccatggctcc
     5641 gatcagatgt gtcgcccgca cgtcgccgga tttgagctga gtttccgacg atgaatgcgc
     5701 ggatgaggcg tcttgcccta gggcgacacc gcagaacaac gcggccgacg ccagggtgag
     5761 taccgattga ttaattattc gcatcgtatc gctccttgat acttacgaga gagagtgggg
     5821 atgctgccaa agcaagtgtc gtgccacttg gcgagacaga cgggcggcat cgcgcgcgat
     5881 ttctcttcgc gccaggctct ttaggagtcc ttcttggccg gcgcctgcga caatccgtcg
     5941 catcctcgaa ggccgctgcc gtttctatac tttctacatc gaccttgcac cgcctcgggg
     6001 atctccatgc gcattcgctt gacctgcatc gcggccgccg tggcgtcgct cgcgaatagc
     6061 ggcgagtcgg cggcgcagca gcaggggctc gataccatcg tcgtgacggc ggaacgcatt
     6121 caggcggatc tcgaggccga gcgcgccgtc acgcccggcg ccgtcacgaa tctcgacggg
     6181 gcgaagttct acgaacgcag ggtctcgcag ctgtccgaca tgttgcgcta cgtgccgggc
     6241 gtttatgccg agagctacaa cggcaacgac gacgtgttct actcgagccg cggctcgaac
     6301 ttggatgcca ccgactacga caagaacggc gtcaagttct tgcaggacgg cctgccggtc
     6361 acggcggccg acggcaacaa ccacaaccgc gcgctcgatc cgctcggcgc gcgctacgcg
     6421 accgtcgcgc acggcgcgaa cgcgctggcg tacggtgcga gcacgctcgg cggcgccgtc
     6481 gatttcacga cgccgaccgc ccactcgacg atgccgagcg tcgctacgtc cgcgggcagc
     6541 ttcggcgagt ggaacttacg cgccacgatg ggcgcagttt tctcggacga gctcgacgga
     6601 ttgatcacgg ccgaagtgca gcagcgcgac ggctatcgcg agcacagcgc gcacgaccgc
     6661 aagggactct atgcgaacct cggctggtcg gtgtcggaga acgtgtcgac gcgtttctac
     6721 gccacgcata tcgactacga ctcgcagctg ccacgcgagc tcactccggc gcagtacgcg
     6781 caagacccta accaggcgcg agccgacgcg atactcggca atcacaacaa gcaagtcgca
     6841 gcgtcgcgcc tcgcgttcaa gaccacgttc gcggaagtcg cggacggcac gctcgagctc
     6901 ggcgtctcct acgagaacca agagctctac catccca
//
LOCUS       DATLEE010000565         4544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5868921, whole genome shotgun sequence.
ACCESSION   DATLEE010000565 DATLEE010000000
VERSION     DATLEE010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5868921"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3804
                     /locus_tag="VM692_12245"
     CDS             <1..3804
                     /locus_tag="VM692_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52986.1"
                     /translation="EIAAYEAAGSTGLAILQGIEGLEGLAGDVLGAFGVDNSPGPSEQ
                     LLKTITAGLDKICTAARSDCAYMALPWAGLAAAPGNSGGFRLGARMFLPTDVAVLGDG
                     KFLIADSGNERIKTVGIDFSGTEPKDAPYIVANTDQLQDYPFAVAAMGNDTAVAATSA
                     DSTLSKVTLRDGSSVLSRFAGVTKAFRCTRAEGDVRQPLGVPMGIATGESGTFVADPY
                     CATIWQLDANGEAHDIRGDLKLPLNPLPPCSDGPLAFATFGAPMDVAVQSDGSIWVAD
                     SWCHSIRVIRDRLAGVEDTAAEAAKWVDGITGWFGEEHATDLRARLDNATLGALDPAR
                     WWVTTIAGSPDGEPGFRDGAASEALFNAPAAIALAEDDDGKAYLFVSDVGNRRIRRID
                     LNARSETTRVPPEVLEAPPFRLPDADDEPLSPLDAVNFLIAWVYNRNAAQAAADATDA
                     ILALDDRYLAKASDVTNGVAWEMSLRFGDVTPDVSLSSPPGFTSASRDGFAMAAPLVG
                     TWRAGFSANLHGKARASSGGVSLFSWTPEVFDFGLGVRDLRVTANVDLDAAPAVPQLQ
                     SATVHAQALLYGTGVLEFNRPIDLTAQPRDGALELRGQLADERLDLEGLSARLDADLV
                     LTIRPSPNEIEVVVDPVEGDALVDERIPTTRVTVAIKGTLKTELDGVGEVGVPLAAEF
                     DVSMPSTDTLDVALRGLERPIPRRWGEGSPRFEIDPSKTPSAATFASVATEVESAFRA
                     DHAPLGVVMSRLRPPLSAPALVDTLGSVSTEAVPQGQTRGTGAQSVQAAAQTTAAVRP
                     SAPLAQQVGTAPQQAISFPDPKWMGEEDSAIWTAHYLAAESYRYAAQPSADALAGVQL
                     ALGGIEALFDVTEGAVAVKKGTSAPVRATGILSRTLMSGEPEFQRLNFAEPLNERDCY
                     YVKTGASRAERSAATPAPAGGVRYGYGCSDVQPVSRDQYIGVLMGLTVANRLVDDAAI
                     KQRTERLLERAIAFLVDNEWNIHLPPEERIRTNFIGNFEKQLAILRIGKTLDPDKYAV
                     LYDYVARASELAWLPAWVSTIDIRQQYYKFNLLQSGLVTALLLEDDPALRANYLAVMD
                     LAWGAVGHHRNAYFGLLRALARSPTERNAVLDGPSAYNVQNRITLRQEIPAVLGEWVA
                     RRSIPQVRSPAGLMFNRVPDVGFLLSLWPSDVGEYVTLEGNDVVLAANALPVDKRPSR
                     ESMDFLWQRQPFEVAMRNDIGRTDTPTEQEILDKGERGGDYWREGPGVDYLIAYWLAV
                     YLKVI"
     gene            complement(3797..4390)
                     /locus_tag="VM692_12250"
     CDS             complement(3797..4390)
                     /locus_tag="VM692_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HUQ52987.1"
                     /translation="MAVRQPELFSNQPGLPAGFRYEEDFLSPAAEAALLSDLERLAFA
                     NARYKEWTAKRRIVSYGGRYDFSTSELLPASPVPPFLVDLRAAAAGFAGATAAELTHA
                     LVAEYPAATQLGWHRDVPEFELVVGISLKGAARMRLRRYPHVAGSRERSLAVELAPRS
                     IYCLQGEARWGWQHAISPTPGLRYSITFRSLRRADAR"
BASE COUNT          773 a         1536 c         1527 g          708 t
ORIGIN      
        1 gagatcgcgg cctacgaggc cgcgggatcg acgggcctcg cgatcctgca agggatcgaa
       61 gggctcgagg gactggccgg cgacgtgctc ggcgcgttcg gcgtcgacaa ttccccaggc
      121 ccgagcgagc agctgctgaa aacgatcacg gcggggctcg acaagatctg cacggcggcg
      181 cgcagcgatt gcgcgtacat ggcgctgccc tgggccggac tagccgccgc accgggcaac
      241 tcgggcggct tcaggctcgg tgctcgcatg ttcttgccga ccgacgttgc ggtcctcggc
      301 gacggcaagt tcctgatcgc ggactccggc aacgaacgca tcaagacggt cggcatcgat
      361 ttttcgggca ccgagccgaa ggatgccccc tacatcgtcg cgaacaccga tcaactgcaa
      421 gactatccgt tcgcagtcgc ggcaatgggc aacgacacgg cggtcgcggc aacctcggcg
      481 gattcgacgc tctcgaaggt cacgctgcgt gacggcagct cggtgctgag ccgcttcgcc
      541 ggcgtcacca aagcctttcg ttgcacgcgt gccgagggcg acgtgcgtca gccgctcggc
      601 gtgcccatgg gcatcgcgac tggcgagtcg ggcaccttcg tcgccgaccc ctactgcgcg
      661 acgatctggc agctcgatgc caatggcgaa gcgcacgaca tacgcggcga tctgaagttg
      721 ccgctcaatc cgctaccgcc atgctcggac gggccgctcg cgttcgcgac attcggcgcg
      781 ccaatggacg tcgcagtgca atccgacggc agcatctggg tcgccgattc gtggtgccac
      841 tcgatccgcg tgattcgcga tcggctggcc ggcgtcgagg acacggccgc cgaagcggcc
      901 aaatgggtcg acggaatcac gggttggttc ggcgaggagc acgccacgga cctccgcgct
      961 cgactcgaca acgcgaccct gggcgcgctc gaccccgcgc gctggtgggt aacgacgatc
     1021 gccggctcgc ccgatggcga gcccggtttt cgcgacggcg cggccagcga agcgctgttc
     1081 aacgcgccgg ccgcgatcgc gctcgccgag gacgacgacg gcaaggcgta cttgttcgtc
     1141 tcggacgtcg gcaaccgacg catccgccgc atcgacctga acgcgcggtc cgagacgacg
     1201 cgcgtgccgc ccgaggtgct cgaggcgccg ccgttccgct tgcccgacgc cgacgacgag
     1261 ccgttgtcgc cgctcgacgc ggtgaacttc ttgatcgcgt gggtctacaa ccgcaacgcc
     1321 gcgcaagccg ctgccgacgc gaccgacgca atccttgcgc ttgacgatcg ctacctcgcg
     1381 aaggccagcg acgttacgaa cggcgtcgcg tgggagatga gcctgcgatt cggcgacgtc
     1441 acgcccgacg tgagcttgag ctcgccgccg gggttcacga gcgcctcgcg cgacggcttc
     1501 gcgatggcag cgccgctcgt cggcacctgg cgggcgggct tcagcgccaa tctgcacggc
     1561 aaagccagag cgagctcggg cggcgtgagc ttgttttcgt ggactcccga ggtgttcgat
     1621 ttcgggctcg gtgtccgcga cctgcgcgtg accgcgaacg tcgatctcga cgcggcgccg
     1681 gcggtgccgc agctccagtc cgcgacggtg cacgcacaag cgctgctcta tggcaccggc
     1741 gtgctcgagt tcaaccgccc gatcgacttg accgcgcagc cgcgcgacgg tgcgctcgag
     1801 ctgcgcgggc aattggccga cgagcgtctc gatctcgaag ggctgagcgc aaggctcgac
     1861 gccgacctcg tgttgacgat ccgcccgtcg ccgaacgaga tcgaggtcgt cgtcgatcct
     1921 gtcgaaggcg acgcgctcgt cgacgagcga attccgacga cgcgcgtgac ggtcgcgatc
     1981 aaaggcacgc tgaagacgga gctcgacggc gtcggcgagg tcggcgtgcc gttggccgcg
     2041 gagttcgacg tttcgatgcc gtcgaccgac acactcgacg tcgcgctgcg cggcctcgag
     2101 cggccgatcc cgcgccgctg gggcgaaggc tcgccgcgct tcgagatcga cccctcgaag
     2161 acaccttcgg cggcgacgtt cgcaagcgtc gccaccgagg ttgaaagcgc attccgcgcc
     2221 gaccacgcgc cgctcggcgt cgtgatgtcg cgcttgcggc caccgctgtc ggcgcccgcg
     2281 ttggtcgaca cgctcggctc cgtgagcacc gaagcggtgc cgcaaggcca gacgcgaggg
     2341 acgggcgcgc agtcagtgca agccgccgcg caaacaaccg ccgccgtgcg gccgtcggcg
     2401 ccgctcgcgc agcaggttgg tacagcgccg caacaggcca tttcgtttcc ggatcccaaa
     2461 tggatgggcg aagaggactc cgcgatttgg acggcgcact acctcgccgc cgagtcgtac
     2521 cgctatgccg cgcaaccgag cgccgacgcg ctcgccggag tgcagttggc actcggcggc
     2581 atcgaagcat tgttcgacgt caccgaagga gccgtggcgg tcaagaaagg aacctccgcg
     2641 ccggttaggg ccacggggat cttgtcgcgc acgttgatga gcggcgagcc cgagttccag
     2701 cggcttaact tcgcggagcc gctcaacgag cgcgactgct actacgtgaa gaccggcgcg
     2761 agccgcgccg agcggtcggc cgcaaccccc gcgcctgccg gcggcgtccg ctacggctat
     2821 ggttgcagcg acgtgcaacc cgtcagccgc gaccaataca tcggtgtgct gatggggctg
     2881 acggtcgcga accggctcgt cgacgacgcg gccatcaagc agcggaccga gcgcctcctc
     2941 gagcgcgcaa tcgcgtttct cgtcgacaac gaatggaaca tccatctgcc gcccgaggaa
     3001 cgcattcgaa cgaacttcat cggcaacttc gagaagcagc tcgcgatcct gcgaatcggc
     3061 aagacgctcg atcccgacaa gtacgccgtg ttgtacgact acgtcgcgcg agcgtcggag
     3121 ctcgcgtggt tgcctgcctg ggtcagcacg atcgacattc ggcagcagta ctacaaattc
     3181 aatctgctgc aatcggggct agtgaccgcg ctgctgctgg aggacgatcc ggcgctgcgc
     3241 gccaattacc tcgcggtcat ggatctcgcg tggggcgccg tcggccatca tcgcaacgcc
     3301 tattttggct tattgcgtgc gctcgctcgt tcgccgaccg aaaggaacgc cgtgctcgac
     3361 gggccttccg cctacaacgt gcaaaaccgg atcacgttgc ggcaggagat tccggccgtg
     3421 ctcggtgagt gggtcgcgcg ccgctcgatt ccgcaagtcc gctcgccagc cgggctcatg
     3481 ttcaatcggg ttcccgacgt cgggttcctc ttgagcctct ggccgagcga cgtcggcgag
     3541 tacgtcacgc tcgaaggtaa cgacgtcgtc ctcgccgcca acgcgctacc cgtggacaag
     3601 cggccgagcc gcgagagcat ggattttctg tggcagcgcc aaccgttcga ggtggccatg
     3661 aggaacgaca tcggccgaac cgatacgcca accgaacaag aaatcctcga caaaggcgag
     3721 cgcggcggcg actactggcg cgaagggccc ggcgtcgact acttgattgc gtattggctc
     3781 gccgtttacc tgaaggtcat ctagcgtcgg cacggcgcaa gctgcggaac gtgatcgagt
     3841 agcgcaggcc cggcgtcggc gagatcgcgt gctgccaacc ccagcgcgcc tcgccttgca
     3901 agcagtagat cgagcgcggc gccaactcga cggcgagcga ccgctcgcgg ctacccgcca
     3961 cgtgcggata gcggcgcaac cgcatgcgcg ccgccccctt cagcgagatg ccgacgacga
     4021 gctcgaactc cggcacgtcg cgatgccagc cgagctgcgt cgcggcggga tattcggcca
     4081 cgagcgcatg cgtgagctcg gcggccgtcg cgcccgcgaa gccggctgcc gcggcgcgca
     4141 agtcgactaa aaacggcgga acggggctcg cgggcaagag ctcgctcgtg ctgaagtcgt
     4201 agcggccgcc atagctgacg atgcggcgct tggccgtcca ctccttgtag cgcgcgttgg
     4261 cgaacgccag ccgctcgagg tcgctcagca gcgcggcttc tgcggcgggc gacaaaaagt
     4321 cttcctcgta gcggaaacct gccggcaaac cgggctggtt cgagaagagc tcgggctgtc
     4381 gaacggccat gggcacggtt ggagcgcctc gccggcgtta acgttccccg cgtgcgctaa
     4441 agtgttgttt cagcgccgcc gagagaccaa cgtcgatgtt cgtcgtagag ctcatttaca
     4501 aagccgacct cgccgagatc gacggcaaaa tgcgcgctca catg
//
LOCUS       DATLEE010000566         3006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10020542, whole genome shotgun sequence.
ACCESSION   DATLEE010000566 DATLEE010000000
VERSION     DATLEE010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10020542"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VM692_12255"
     CDS             <1..544
                     /locus_tag="VM692_12255"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ52988.1"
                     /translation="AVPDAPVGIPADVDEQINLMFDLLALAYQADLTRVATLRIAAEQ
                     TVRTYTNLGISDAFHPISHHGHRHENYAKLIKIQTYHSQVVARFLARLDGFTEADGSS
                     VLDNSVLLYGSGMSDSDLHNSSPLPLAVFGRGGGRIKGNQHLAYAEDTPYNNFLLTLL
                     ERAAVPAESVGNSTGDLAEV"
     gene            547..2205
                     /locus_tag="VM692_12260"
     CDS             547..2205
                     /locus_tag="VM692_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ52989.1"
                     /translation="MKKRVAFAAALLVGAGGAASADALIDAVRAGNGEEAVRLIENGA
                     DANAADSLGTTPLMWAARYGDAAVVKRLIRAGAKPAAENVFGVTPMSEAALIGSEPVI
                     RELLAAGVDPDSPNPEGETALMLAVRTGQLEAAKALIDAGADVNARERWAGQTALMWA
                     GAQLQPQMVKLLLAHGAEVNARSTVREWTRKVSSEPRPKELAQGGLTPLMFAARTGCI
                     DCAELLLAAGADINLTDPSGVTPLIVATLNLQNDFAKYLVEKGADINEWDLYGRTALY
                     VAIDMMDYPPPRVSGGPAEPMTGLRLAEFLLDRGANPNAQLKQWRPPFVRLARGQDNT
                     LATGATPLLRAAHSSDVAAVKLLLAHKALVDLPNASGITPLMAAAGVGVSQNTSRAKK
                     KTEAASIEVARLLLEAGADVNRVTHDPRRIRQDPLVREAMYGFIFQMAFDYAYLPPSG
                     RTALHGAAQKGWNDMVRLLVEHGAKVQPVDATGRTPRNMAMGNYELAITEARPDPLTE
                     TVALLEQLCREDGDCDPKAGCDLDCSTFAVPPVESQLNQTVRLN"
     gene            2220..2885
                     /locus_tag="VM692_12265"
     CDS             2220..2885
                     /locus_tag="VM692_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HUQ52990.1"
                     /translation="MLTVSRRILIAIGAVSSAAVAIVSYRYLWPSLPVSGLVATNAFG
                     VPWLTVHAAAAATALLVGPLQFRSQLRARRPKLHRVLGRVYVTGCVVGGATGLLLALG
                     TTAGPVATAGFGSLAVVWLVATARAWRLAVRRVLAQHREWMVRSFALTFAAVTLRIYL
                     PISQTLPVELDDAYRAISFLCWVPNLLVAELYLRTRRGRGSSVDQRVASPIKMTPTKI
                     INQ"
BASE COUNT          481 a          980 c         1028 g          517 t
ORIGIN      
        1 cgccgtgccg gatgcgcccg tgggcattcc ggccgacgtc gacgagcaga tcaacctcat
       61 gttcgatttg ctggctctgg cttatcaggc cgatctgacg cgcgtggcta cgctgcgaat
      121 cgccgccgag caaaccgtca ggacctacac gaacctcggc atctcggacg cgttccatcc
      181 gatctcgcat cacggccatc ggcacgagaa ctacgcgaag ctgatcaaga tccagacgta
      241 ccacagtcag gtcgtcgcgc gcttcctcgc gaggctcgac ggcttcaccg aggccgacgg
      301 ctcgtcggtg ctcgacaact ccgtgctgtt gtacggcagc ggcatgagcg acagcgacct
      361 gcacaactcg agcccgttgc cgctcgctgt ttttgggcga ggcggcggcc gcatcaaggg
      421 caatcagcat ctcgcgtacg cggaagacac gccgtacaac aactttttgc tgacgctgct
      481 cgagcgcgcg gcagtgcccg cggagtccgt cggcaacagc accggcgatc tggcggaggt
      541 atagcgatga agaagcgagt cgcgttcgct gcggcgttac tggttggcgc cggcggcgct
      601 gcgtcggccg acgcgctcat cgatgccgtt cgtgccggca acggcgaaga ggccgttcgc
      661 ctcatcgaga acggggcgga cgcgaacgcg gcggattcgc tgggcactac gccgctgatg
      721 tgggcggcgc gttatggcga cgccgccgtc gtcaagcggt tgatccgggc gggggccaag
      781 cctgcggccg agaatgtctt cggcgtgacg ccgatgtcgg aagccgcgtt gatcggctcg
      841 gagcccgtga tccgcgagct gttagcggcc ggtgtcgatc ccgattcgcc gaaccccgaa
      901 ggtgaaacgg cgttgatgct cgcggtacgc acagggcagc tcgaagcagc caaggcgctg
      961 atcgatgccg gcgccgacgt caatgccaga gagcgctggg caggtcagac ggcgttgatg
     1021 tgggccggcg cgcaattgca gccgcagatg gtgaagctgt tgctcgcgca cggcgcggag
     1081 gtcaatgcgc gttcgaccgt gcgcgagtgg acccgcaagg tcagtagcga gccgcggccg
     1141 aaggaattgg cgcagggcgg tctcacgccg ctgatgttcg cggcgcgcac cggctgcatc
     1201 gattgcgccg agctgctgct cgccgccggc gcggacatca atctgacgga cccgtccggc
     1261 gtcacgccgt tgatcgtcgc cacgctgaac ctgcaaaacg atttcgccaa gtacctggtc
     1321 gagaagggcg ccgacatcaa cgagtgggat ctctacggtc gcaccgcgct gtatgtcgcg
     1381 atcgacatga tggactatcc gccgccacgc gtatcgggcg gcccagccga gccgatgacg
     1441 ggcttgcggc tcgccgagtt tctgctcgac cgcggcgcca acccgaacgc ccagctcaag
     1501 cagtggcgcc cgccgttcgt gcggttggcg cgcggccagg acaacacgtt ggcgacgggc
     1561 gcgacgccgc tgctgcgggc tgcgcactcg agcgatgtcg ccgcggtgaa actgctgctc
     1621 gcgcacaaag cactggtcga tctgccgaac gcttcgggca tcactccgct gatggccgcc
     1681 gcgggcgtcg gtgtcagcca gaacacgagc cgtgccaaga agaaaaccga agccgcctcg
     1741 atcgaggtgg cacggctact gctcgaggcc ggtgccgacg tcaaccgagt cacgcacgat
     1801 ccgcgccgga tccgccaaga tccgctcgtt cgcgaggcga tgtacggctt catctttcag
     1861 atggccttcg attacgcgta cctcccgccg agcggccgga cggcgttgca cggcgccgcg
     1921 cagaaaggct ggaacgacat ggtgcgcctg ctcgtcgagc acggcgcgaa ggtgcagccc
     1981 gtggacgcga cgggccgaac tccgcgcaac atggccatgg gcaactacga gctcgcgatc
     2041 accgaggcgc gtccggatcc gctgacggag accgtagcgc tgctcgagca actctgtcgt
     2101 gaggacggcg actgcgatcc aaaggccggc tgcgacctcg attgcagcac gttcgcggtt
     2161 ccacccgtcg agtcgcagct caatcaaacc gtccgcctga actaagcttg tccgttgccg
     2221 tgttgacggt ttcgcgccgg atcctcatcg ctatcggcgc cgtatcgagc gccgctgtgg
     2281 ccatcgtctc gtaccgctat ctctggccga gcctgccggt ttccgggctc gttgcgacga
     2341 acgctttcgg cgtgccttgg ctcaccgtgc acgcggccgc ggcggctacg gcgctgctcg
     2401 tcgggccgct acagtttcgg tcgcagcttc gtgcgcggcg gccgaagctg caccgcgtgc
     2461 tgggccgcgt gtacgtgacg ggttgcgtgg tcggaggtgc gacgggtttg ctgctggcgc
     2521 ttggcacgac ggcagggccg gtggcgacgg ccggtttcgg ctcgctggca gtcgtttggc
     2581 tcgttgcgac ggctcgcgcg tggcggctcg ccgtgcggcg cgtgctcgcg cagcatcgcg
     2641 agtggatggt ccgctccttc gcgctgacgt ttgcggcggt gacgctgcgt atctatctgc
     2701 cgatttcgca gacgctgccc gtcgagctcg acgacgcgta ccgcgcgatt tcgttcttgt
     2761 gctgggtgcc gaacctgctc gtagcggagc tctacctgcg cacgcgtcgc ggtcgcggct
     2821 cgtcggtcga tcagcgcgtg gcttcgccga tcaagatgac gccgacgaag atcatcaacc
     2881 aatagatgat cctgtaaaag cgctcgccgt cgatgcggcg cacgagccag acgcccgcga
     2941 acgtcgacac gatcgcgagc ggcagcagcg ccgcggccgt cgacatgttg gtcgcgttga
     3001 attggc
//
LOCUS       DATLEE010000567         4175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1405723, whole genome shotgun sequence.
ACCESSION   DATLEE010000567 DATLEE010000000
VERSION     DATLEE010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405723"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VM692_12270"
     CDS             <1..266
                     /locus_tag="VM692_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52991.1"
                     /translation="FVLLLADVDTAEAEAVVARMQATIESWFRESRFRCGISIGFAPA
                     SAADWDLEQVLKSVDRLLYQSKALRNSGDAATRPVGDSSSDPA"
     gene            298..1584
                     /locus_tag="VM692_12275"
     CDS             298..1584
                     /locus_tag="VM692_12275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ52992.1"
                     /translation="MGAAIGIEASGTSATPWTLRRVVTAGVIGNVLEWYDFAVYGFFA
                     PILAAKFFPAEDPRVSLLAAFAAFAVGFLMRPVGAAVFGHIGDRYGRARALLFSIAMM
                     AVPTMLMGLLPTYDSIGIAASVLIVALRMCQGIAVGGEFTASIVFLAENAPAPRRGFF
                     ASFSMFGATTGTMLGSAVGGLLTSVLSADALASWGWRAAFVSGIAVAAVGIVIRRGMF
                     DSPSKATVASPLAVAFRDHRWQVLRVLGLNVASAATYYTLFVYAATWVSQTTSLERAV
                     ALNITTVTILTFLVVLPCAAWLSDRFGRKLMLVIGMSACLLAAYPLLTAMHSGALATT
                     APAQMAFGGLLALQMASIPAAMTEMFPHGVRVSAVSVGYGLAYALFGGTAPAVATWLI
                     TRTGNDTVFAWYLMALTAISLVLALSITDRRGQPLS"
     gene            complement(1612..2214)
                     /locus_tag="VM692_12280"
     CDS             complement(1612..2214)
                     /locus_tag="VM692_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUQ52993.1"
                     /translation="MTRMLGAVGVVLALIGCTPADEPPPRADAAAATSGTPAPALEGQ
                     GWTGLTDPDEVIEARRALMDEAERLMKPIDEYSIGLPHDAESLHSAATTLERLLLALP
                     HLFPPTTNQYDPDAHDPLTIALPAIWQRFAAFQSFADSAEHAAAALATAEDGEPLRAA
                     STRMRAACDTCHAAFMKPYTPPQVSDEDREFDFDSVLPPN"
     gene            2353..3171
                     /locus_tag="VM692_12285"
     CDS             2353..3171
                     /locus_tag="VM692_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ52994.1"
                     /translation="MRTSPRHFRTSFATLFALAALLAACSGEAQPPVHPLVMTSVSPA
                     HCVQALTSGGPADLIRCPAPLRDALAEAMAVCKDAGGTLVGIPEGDVWSLDVNGDGRQ
                     ELLFEIDSNVGCEGAFSIFSCGSLGCPKALYELRNGAWTVVGSLSAVAPENVTLARTA
                     ASDGHRALEVCAQQNCTELWTYEWRDSMYETTRLKVRGAGVDFAGSIHGLYPLAAATT
                     VRATPAQRGAEVGNYDARTEVAIIGTAEGADYYYVSPCNACESGFVPRSAITVP"
     gene            3183..4061
                     /locus_tag="VM692_12290"
     CDS             3183..4061
                     /locus_tag="VM692_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HUQ52995.1"
                     /translation="MTSTRSARFRELLKKPPFVCMGAHDAVTAKLAEHAGAPAIYVSG
                     FVASAIVAGAPDFGVLTQTEMFEHIRRICRVTSVPVFADADTGYGGVLDAQRTIQLWE
                     EAGASVLHLEDQAMPKKCGHFAGKQLISAEEMQQKLRAMVAARRDPDFFIVARTDAMG
                     VTDLDDAIRRLEMYAAVGADGLYVDAPRSVEQLKEIARRLKPLGLPLLFNMVRSGKSP
                     YLTLKEVHALGFDYALCPVEPMLAMHKAVKEMMTTFMREGCSTNAIADRLTPFHEFNE
                     FIGLDEIVKREKQFAP"
BASE COUNT          647 a         1401 c         1387 g          740 t
ORIGIN      
        1 agtttgtcct gctcctcgcc gatgtcgaca ccgcggaagc ggaggccgtc gtcgcgcgca
       61 tgcaagccac gatcgaatcg tggttccgcg agagccgatt tcgctgcggc atcagcatcg
      121 gcttcgcccc ggcgtcagcg gcggactggg atttggagca agtgctgaag tccgtcgatc
      181 ggttgctgta tcaatccaag gccttgcgca attcgggcga cgcagcaacg cggccggtcg
      241 gtgatagcag cagcgatccc gcataggggt tgctcagaac aggtacactc gcgtcgcatg
      301 ggggcagcga taggcatcga ggcgagcggg accagcgcga cgccgtggac actgcggcgc
      361 gtcgtcacgg ccggcgtgat cggcaacgtg ctcgagtggt acgacttcgc cgtgtacgga
      421 ttcttcgcgc cgatcctcgc cgccaagttc ttcccggcag aggacccgcg cgtatcgctg
      481 ctggccgcgt tcgcggcgtt cgcggttggt ttcttgatgc ggcccgtagg agctgcggtc
      541 ttcgggcaca tcggcgaccg ttacggccgc gcccgcgcgc tgctcttctc catcgcgatg
      601 atggccgtgc cgacgatgct gatggggctg ctgccgactt acgactcgat cggcatcgca
      661 gcgtcggtgc tgatcgttgc actgcgcatg tgccagggca tcgccgtcgg cggcgagttc
      721 acggcctcga tcgtgttcct cgccgagaac gcaccggcgc ctcgccgcgg gttcttcgcg
      781 agcttctcga tgttcggcgc gacgaccggc acgatgctcg gctccgccgt cggcggcctg
      841 ctgacgagcg tgctgagcgc cgacgcgctc gcaagctggg gttggcgcgc ggcgttcgtt
      901 tccggaatcg ccgtggccgc ggtcggcatc gtcattcgcc gcggcatgtt cgatagcccg
      961 agcaaggcga ccgtcgcctc gccgctcgcc gtcgcgtttc gcgaccatcg ttggcaagtg
     1021 ctacgcgtcc tcgggctcaa cgtcgcgtcg gcggccactt actacacgct gttcgtctac
     1081 gccgcgacct gggtgtcgca aaccacttcg ctcgagcgcg ccgtcgcgct gaacatcacg
     1141 acggtcacga tcctgacctt cctcgtcgtg ctgccgtgcg cagcctggtt gtcggaccgg
     1201 ttcggccgca agctcatgct cgtcatcggc atgagcgcgt gcctgctcgc ggcctatccc
     1261 ctgttgacgg cgatgcactc gggcgcgctc gcgaccacgg cgcccgcgca aatggcgttc
     1321 ggtgggctgc tcgcgctgca aatggcgtcg atcccggccg cgatgaccga gatgtttccg
     1381 cacggcgtgc gcgtcagcgc cgtcagcgtc gggtacgggc tcgcgtacgc gctgttcggt
     1441 ggcacggcac cggccgtcgc gacctggcta atcacgcgga ccggcaacga cacggtgttc
     1501 gcgtggtatt tgatggcgct gaccgcgata tcgctcgtgc tggctctgag catcaccgac
     1561 cggcgcgggc agccgttgtc ctgatgcgcg ggttccacgc ctaagcttgc ttcagttcgg
     1621 cggcaacacc gagtcgaaat cgaactcgcg atcttcgtcg ctgacttgcg gcggcgtgta
     1681 cggcttcatg aacgccgcat ggcacgtatc gcacgccgcg cgcatccgcg tgctggccgc
     1741 gcgcaagggc tcgccatcct cggccgtcgc gagcgccgcg gccgcgtgct ctgcggaatc
     1801 ggcaaagctt tgaaaggccg cgaagcgttg ccagatcgcc ggcagcgcga tcgtcagcgg
     1861 gtcgtgcgcg tcggggtcgt attgattcgt cgtcggtgga aatagatgcg gcagcgcaag
     1921 cagtaaccgc tcgagcgtcg tcgcggccga gtgcaacgac tccgcgtcgt gcggcaaccc
     1981 gatcgaatat tcgtcgatcg gcttcatgag tcgttcggcc tcgtccatca gcgcgcggcg
     2041 cgcctcgatg acctcgtcgg gatcggtcag ccccgtccag ccctgcccct cgagcgccgg
     2101 cgccggcgtt ccgctcgtcg cagctgccgc gtcggcgcgc ggcggtggtt cgtcggccgg
     2161 cgtgcaacca atcagggcca acacgactcc aactgcaccg agcatgcgcg tcatgggcct
     2221 ctccttctac gagctcatcg tcgagccgaa cccgtcgggt tgcaataaac gggccttgcg
     2281 gaaacccgcg attgacgcga gcgcgctcgg cgatgccggc ctcagctaga atgaagaaaa
     2341 agggatgtag cgatgcgcac gagccctcgc cactttcgca cgagcttcgc aaccctgttc
     2401 gctttggcag cgctgctcgc ggcatgtagc ggcgaagcac agccgcccgt gcatccgctc
     2461 gtcatgacga gcgtctcccc cgcgcactgc gtgcaagcgc tgacgagcgg cggccccgcc
     2521 gatctcattc gctgccccgc gccattgcgc gacgcgctcg cggaagcgat ggcggtctgc
     2581 aaggacgccg gcggcacgct cgtcgggata ccggaaggcg acgtttggtc gctcgacgtc
     2641 aacggcgacg gccgccagga gctcctgttc gagatcgaca gcaacgtcgg gtgcgaaggc
     2701 gcgttcagca ttttctcgtg cggcagtctc ggctgcccga aggctctcta cgagctgcgc
     2761 aacggcgcct ggaccgtcgt cggcagcctc tcggcggtcg cgccggaaaa cgtgacgttg
     2821 gctcgcacgg ccgcgagcga tggtcaccgt gcactcgagg tctgcgcaca acaaaactgc
     2881 acggagcttt ggacctacga atggcgcgac agtatgtacg agacgacgcg cctgaaagtt
     2941 cgcggagcag gcgttgactt tgcgggctcg atacacggcc tctatccgct tgcggcagcc
     3001 actaccgtgc gcgcgacgcc ggcgcagaga ggcgctgagg tcggtaacta cgatgcgcgc
     3061 acggaggtcg cgatcatcgg cacggccgag ggcgcggact attattacgt ttcgccgtgc
     3121 aacgcctgcg agagcggctt cgtgccgcgc tctgccatca ccgtcccgta aggagtccag
     3181 cgttgacctc gacccgttcc gctcgctttc gcgagctgct caagaaacca ccgttcgtct
     3241 gcatgggcgc gcatgacgcc gtcacggcga agctggccga gcacgccggc gcgcccgcta
     3301 tctacgtcag cggcttcgtc gcctcggcga tcgttgccgg cgcgccggat ttcggcgtgc
     3361 tgacgcaaac cgaaatgttc gagcacatcc gccgcatctg ccgagtcacg agcgtgcccg
     3421 tgttcgccga tgccgacacg ggttacggcg gcgtgctcga cgcgcaacgt acgattcagc
     3481 tctgggagga agccggcgcg tcggtgctgc acctcgaaga ccaggcgatg ccgaagaagt
     3541 gcgggcactt cgccggcaag cagctgatca gcgccgagga gatgcaacag aagctgcgcg
     3601 cgatggtcgc ggcgcgccgc gatcccgatt tcttcatcgt cgcgcgcacc gacgcgatgg
     3661 gcgtcacgga tctcgacgac gcgatccgcc ggctcgagat gtacgcggcc gtcggcgccg
     3721 acgggctcta tgtcgacgcg ccgcgcagcg tcgagcagct aaaggagatc gcgcgccgct
     3781 tgaagccgct cggcctgccg ctgctgttca acatggtgcg ctccggcaag agcccgtatc
     3841 tgacgctcaa ggaagtgcac gcgctcggct tcgattacgc gctctgcccc gtcgagccga
     3901 tgcttgcgat gcacaaggcc gtcaaggaga tgatgacgac gttcatgcgc gagggttgct
     3961 cgacgaacgc gatcgccgac cggctcacgc cgtttcacga gttcaacgag ttcatcggcc
     4021 tcgacgagat cgtcaagcgc gagaagcagt tcgcgccttg acgatgccgc agctactcgt
     4081 cacgcggcgc gagcaggtgc tcgtgctcac gctgaaccgg cccgaggccg gcaatcgtct
     4141 cacgacctcg cttgctaccg acgttgcgca agcgc
//
LOCUS       DATLEE010000568         8414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2898568, whole genome shotgun sequence.
ACCESSION   DATLEE010000568 DATLEE010000000
VERSION     DATLEE010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2898568"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VM692_12295"
     CDS             complement(<1..767)
                     /locus_tag="VM692_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008559161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUQ52996.1"
                     /translation="MDSHKLKIAPGRFAQLLLLSGALVLAATCRAQDVSVCLVTKTDS
                     NPFFVKMREGAQAKAAELGVQLTALAGREEGDNEGQIAAMESCIARGVDGILLVPTDS
                     SAIVATVENARAEGVLVIALDTPLEPIDAADATFATDNFKAGELIGAWARARLGAAAA
                     TAKIAYLNLLPSQPSVDVLRNQGFMQGFGIALADPRRIGDERDPRNACHDVTYGNPEG
                     GRRAMENCLLREPNIRVVYAFNEPAAAGAHEALRALG"
     gene            974..2563
                     /locus_tag="VM692_12300"
     CDS             974..2563
                     /locus_tag="VM692_12300"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HUQ52997.1"
                     /translation="MRTQSLNRTSATLKAAPFVAVSGLVMAALAAPTAVQAQTRDEQA
                     CAALTSRSAGPEDLTITEARFYANRSVAARPGAETTLPPHCHVAGSFERRTGVDGKEY
                     AIGFAVNMPADWNGRFMFQGGGGLNGAIREPVGSQAAGERSALERGFAVVATDSGHQG
                     GGFDGSFMADQQALLNFQFQANAKTAEVALPIVEAYYGKAPHHKYFVGCSTGGREGMI
                     MAQRYPYLFDGIVSGAPAMRTTLSNLALRWISNQYVKAKVETPRDPFTPEEEKLVMSS
                     LMQRCDALDGQADGLIFNRTSCHFDPTELACSASSRAPQCLAANKADALARAMAGPVN
                     AAGLPVYVSYPYDSGIDDAGGLPGLLLAGGSPPIGPNGADLQDQNVDAEFLAAIAANE
                     ALGSTASYYNLTSFIGHGGKQIFYHGEADPWFSANDTVRYFGEIGTANAAVQKVDEYG
                     RLYLVPGMAHCQGGELTPDSFDLVTPLVEWVENDAAPGAVTATGRSMQGRSRPLCPYP
                     SYAHYTRGDAADARSYECRTP"
     gene            complement(2560..3816)
                     /locus_tag="VM692_12305"
     CDS             complement(2560..3816)
                     /locus_tag="VM692_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HUQ52998.1"
                     /translation="MLLLLAQLCAAQTLNDDAAHWVSTWGSAQQLAAEALPPWVVPPP
                     RAPGEPPPPALLPPYPTSFRDQTVRMIVRTSIGGDGVRLTLSNALGMDTVRFGAVHVA
                     LRERDSAIVAATDRAVTFGGRESVSVEPGALVVSDTIALAVPALTELAVSLYLPNETR
                     GVTTHEFALNTTYVAAGNLASAAALPNAATNLTYFWLTGVEVLAPAQAGAIVAFGDSI
                     TDGVTTTPGTQRAWPALLAAKLQSGRATSRWAVINAGISGNRVRRDVIGRSALGRFDR
                     DVLARAGVKWLVLFEGINDVTFSALPDAPQNQRATADDLIAALEQLVDRAHARGIKVM
                     GGTLMPMGGLWLHNAETEAMRQAVNAWIRSSGKLDAVADFDAATRDPLEPARLEPELD
                     SGDHIHPNDAGNAAMAGAIDTMTFAR"
     gene            complement(3853..4944)
                     /locus_tag="VM692_12310"
     CDS             complement(3853..4944)
                     /locus_tag="VM692_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HUQ52999.1"
                     /translation="MQRRSTSMRLVAMGVLGLLLGTAARPQPTPPRQVLVEADGTVHM
                     PAQSVPVSGFLSPEGKAYLAEHLRNLQRPELLVQEDGVPPLLAGFIARQRELFPVERE
                     DVTIGGVHAYVYTPRNRIAPENRDRVLIQLHGGGFSGCWPACAELESLPIAAIGRIKV
                     VSLDYRQGPQHRHPAASEDVAAAYRELTKTYRPENIGLYGCSAGGMLTAMSVAWFQRH
                     ALPRPGAVGILCSGAASVDAGAFGGDASYFTLPLGEGRLLPPGGSNPVPMEYFASTDY
                     DDPLVAPVSSPEVLARFPPTLIVTGTRSFELSNAVYTHAQLVKHGVDADLHVWEGMFH
                     GFFYNPDVPESRECYDVVVRFFADRLGKG"
     gene            5193..6479
                     /locus_tag="VM692_12315"
     CDS             5193..6479
                     /locus_tag="VM692_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HUQ53000.1"
                     /translation="MADLPVLIAGGGIGGLSTALTLHQLGVPCLVLEAVTSLKPLGVG
                     INLQPNAVRELYELGIGPAQLNAIGVQAREWALVGLNGKDVYSEPRGLLAGYRWPQYA
                     VHRGALQMLLHRTAVERLGPSAVRTGHRVAGYRSDADGVTALIKTATGAQRELRGKLV
                     IGADGLHSAVRAQMHPRQPPIHWGGAIMWRGMTPGVPIRSGASFVGLGTHRHRVVFSP
                     ISHADPATGLAMINWIAEITVDSSRGLRDGDWNKKVAIDSFIHHFDGWHYDWLDVPAM
                     LRGAEEVFEYPMIDRDPVPTWVDGRVALLGDAAHVMYPTGSNGASQAIVDARVLGAAM
                     VEHGVTAEALQAYDRKLCAEISAVVLRNRGAGPFGLLNLLDERCGGVFDEIDAVISPP
                     EREEFMSRYRAAAGFAKDRLNAAPPTIAPGARVAAT"
     gene            complement(6517..7344)
                     /locus_tag="VM692_12320"
     CDS             complement(6517..7344)
                     /locus_tag="VM692_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUQ53001.1"
                     /translation="MQRCLLVLTLAWLGIAAVAAHAAPPTGDQHRHYAFAPTGTELPY
                     RLYVPSTWDGERSLPIVLFLHGAGANESTYLDVADGLLRRLAEQHGYVVVSPLGFTPL
                     GAYGNPLRLPAVFGESATAAAQRAAVTPQRRRELALSELEVITVLEIVTEEYGADRSR
                     TFLAGHSMGSGGVWHLAARYPERWRAVAPMSGPGIDKESYPFDRIRKLPVFVTEGTGA
                     TPSLAGSRALAEFMRRGDFDFDYLEVDGDHGGMVPMVWPAVFDFFERVSARGAEIRQ"
     gene            complement(7358..>8414)
                     /locus_tag="VM692_12325"
     CDS             complement(7358..>8414)
                     /locus_tag="VM692_12325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ53002.1"
                     /translation="FTQLGFFTGLYGLLALLLSVPAGLAAKRFGEKRVLAAGLVGVAI
                     GSVLLARAWSFESAVAFRGLTIFGYRFAFVSVLIAVAMTAPPQLRGRTMGVLGATSAM
                     ASVIGAPLGGALVGELGWRTAILGYAAMALLGAAVFFLFYRPSAAPESEPEVATDGAG
                     QPSAFRSPVVWLLALVVGLGGFGQFTVTYFVPSVAASVYGLDAAAAGIIISTGYIAAI
                     VLNLVIGTLTDRFDKLVVLAAIVAVLATASASLALENELTFRIATAVVIGFGFSAANQ
                     LYGLAGSLLPRRETGNAMGIVSLGAGLFGYFGPQMLGILRDATGSFSAGFYMVAVADL
                     LTLGLIVLLYRMTRRAT"
BASE COUNT         1444 a         2811 c         2843 g         1316 t
ORIGIN      
        1 ttgccgaggg cgcgcagcgc ctcgtgcgcg ccggccgccg cgggctcgtt gaacgcatag
       61 accacgcgaa tgttcggctc acgcagcagg caattctcca tcgcgcggcg gccgccctcg
      121 ggattgccgt aggtcacgtc atgacacgca ttgcgcgggt cgcgctcatc gccgatgcgg
      181 cgggggtccg cgagtgcaat gccgaagccc tgcatgaaac cttggttgcg cagcacgtcg
      241 acgctcggct ggctcggcag caggttcaag taggcgatct tcgccgtcgc ggccgcggct
      301 ccgagccgcg cacgggccca cgcgccgatg agttcgccgg ccttgaaatt gtcggtcgcg
      361 aacgtcgcgt ctgctgcatc gatcggctcg agcggcgtgt cgagcgcgat cacgagcacg
      421 ccctcggcgc gcgcattctc gaccgtagcg acgatggcgc tgctgtcggt cggaacgagc
      481 aggatcccgt cgacgccgcg cgcgatgcag gactccatcg ccgcgatctg cccttcgttg
      541 tcgccttcct cgcggcccgc cagcgccgtg agctgaaccc cgagctctgc ggctttcgcc
      601 tgcgcacctt ctctcatctt cacgaagaac ggattcgagt ccgtcttcgt gaccaggcac
      661 acactgacgt cttgagcgcg gcacgttgcc gccagcacga gcgcaccgct cagcaagagc
      721 agctgcgcga accttccggg cgcaatcttc agcttgtgcg agtccatgcc tttagggagc
      781 aagcggggcg tgtccgccgc atgatagtcg ctcatgaggg gtttgcaatc gattgcagac
      841 caagcattcc gcgctatgct gactcgtacg cgcagctgtc cggcgcgcga tcgatgcaag
      901 ccgtgggggc gtgctatgtc gcttgacggt gccgtcgccg gctcgtcatc atcgatcaag
      961 gggggttatt acgatgcgaa cacagtcgtt gaacagaacc agcgcgacgc tgaaggcggc
     1021 gccgttcgtt gcagtgagcg gacttgtaat ggcggcgctc gccgcgccga cggctgtgca
     1081 ggcgcagacg cgcgacgaac aggcctgtgc cgcgctgacg agccggtccg ccggccccga
     1141 ggatctgacg atcaccgagg cgcgcttcta tgcaaaccgc agtgtcgccg ctcggcccgg
     1201 cgccgagacg acgctgccgc cgcactgcca cgtcgccggc agcttcgagc ggcgaacggg
     1261 cgtcgacggc aaggaatacg cgatcggttt cgcagtcaat atgccggccg actggaacgg
     1321 gcggttcatg tttcagggcg gcggcgggtt gaacggcgcg atccgcgagc cggtcggatc
     1381 gcaggcggcg ggcgagcgca gcgccctcga gcgcggcttc gccgtcgtgg ccacggacag
     1441 cggtcatcaa ggcggcggct tcgacggcag cttcatggcc gatcagcagg cgctgttgaa
     1501 ttttcaattt caagcgaacg cgaagacggc tgaggtcgcg ctgccgatcg tcgaggcgta
     1561 ctacggcaag gcgccgcatc acaagtactt cgtcggctgc tcgacgggtg gccgtgaagg
     1621 catgatcatg gcgcaacgct atccgtacct gttcgacggc atcgtttccg gcgctcctgc
     1681 gatgcgaacg acgctcagca acctcgcgtt gcgctggatc agcaatcagt acgtcaaggc
     1741 gaaggtcgag accccgcgcg atccgttcac gcccgaagag gagaagctcg tcatgagctc
     1801 gctgatgcag cgctgcgatg cgctcgacgg tcaggcggac gggctcatct tcaaccgcac
     1861 gtcctgccac ttcgacccga ccgagctcgc gtgctcagct tcgtcgcgag ctccgcagtg
     1921 cttggccgcc aacaaggcgg atgcgcttgc gcgagccatg gccggccccg tgaatgcagc
     1981 cggccttccg gtatacgtct cgtatccgta cgactccggc atcgacgacg ccggcggcct
     2041 gcccgggctg ctgctcgcgg gcggcagccc gccgatcggt cccaacggcg ccgacctgca
     2101 ggaccagaac gtcgatgcgg agtttctcgc ggcgatcgcc gcgaacgagg cgctcggcag
     2161 cacggcgtcg tactacaact tgacttcgtt catcggccac ggcggcaagc agatttttta
     2221 tcacggcgaa gcagacccgt ggttctccgc gaacgacacc gttcgctact tcggcgagat
     2281 cggcactgcc aacgcagcgg tgcagaaggt cgacgagtat ggacgcttgt acctcgttcc
     2341 cggcatggcc cattgccagg gaggcgagct gacgcccgat tcgttcgact tggtgacgcc
     2401 tctcgtcgag tgggtcgaga acgacgcagc gcccggcgcc gtgacggcga cgggccgatc
     2461 gatgcagggg cgcagccggc cgttatgtcc gtatccgagc tacgcgcact acacgcgcgg
     2521 cgacgcggcg gacgcccgca gctacgaatg ccgcacgccc tagcgggcaa acgtcatggt
     2581 atcgatggcc ccggccatcg ccgcgttgcc cgcgtcgttg gggtggatgt gatcgccgct
     2641 gtcgagctct ggctcgaggc gcgcaggttc gagcgggtca cgcgtggcgg cgtcgaaatc
     2701 ggccacggca tcgagcttgc cgctcgagcg aatccatgcg ttgacggcct ggcgcatcgc
     2761 ttccgtctcg gcgttgtgca gccatagccc gcccatcggc atcagcgtac cgcccatgac
     2821 cttgatgccg cgggcatgag cgcggtcgac gagctgctcg agcgcggcga tgaggtcgtc
     2881 ggccgtcgct cgttgattct gtggtgcgtc gggtagtgcc gagaaggtga cgtcgttgat
     2941 gccctcgaac aggacgagcc acttcacgcc ggcacgcgcg agcacgtcac gatcgaaacg
     3001 cccgagcgcg ctgcggccga tgacgtcgcg ccgcactcgg ttgcccgaga taccggcatt
     3061 gatcacggcc cagcggctcg ttgcacggcc cgattgcagc ttcgcggcca gcagcgccgg
     3121 ccacgctcgc tgcgtaccag gcgtcgtcgt gacgccatcg gtaatcgaat cgccgaacgc
     3181 gacgattgcg ccggcttggg cgggggcgag cacctcgacc ccggtcagcc aaaagtacgt
     3241 cagattcgtc gctgcgttgg gcagcgcggc cgcactagcg aggttgcctg cggcgacgta
     3301 agtcgtgttt aacgcaaact cgtgtgtcgt cacgccgcgc gtttcgttgg gcaggtacag
     3361 cgagaccgcc agctccgtca gcgcgggcac cgcgagcgcg atcgtgtcgc tgacgacgag
     3421 cgcgccgggt tccacggaca ccgactctct gccgccgaac gtgacggcac gatccgtcgc
     3481 cgcgacgatc gccgaatcgc gctcgcgtag cgcgacgtgc acggcaccga agcgcaccgt
     3541 atccatgccg agcgcgttcg agagcgtcaa gcgcacgccg tcgccgccga tgctcgtgcg
     3601 cacgatcatg cgaaccgttt ggtcgcggaa cgacgtggga tagggcggaa gcaaagcagg
     3661 cggcggcggc tcgccgggcg cgcgcggcgg cggcacgacc catggcggca acgcctctgc
     3721 ggcaagctgc tgcgccgagc cccacgtgct gacccaatgc gcagcgtcgt cgttcaaggt
     3781 ttgcgcagcg cagagctggg caagcagtag aagcacgaac gctccaagac gttgctgcac
     3841 gtgacctccc gctcagccct tgccgagtcg gtcggcgaag aaacgcacga ccacgtcgta
     3901 acactcgcgc gactcgggga catcgggatt gtagaagaag ccgtgaaaca tgccttccca
     3961 tacatgcaga tcggcatcga ctccgtgctt gacgagctgt gcgtgcgtgt acacggcgtt
     4021 gctgagctcg aacgaccgtg ttccggtgac gatcaaagtc ggcgggaaac gcgcgagcac
     4081 ctcgggtgag ctgaccggcg cgacgagcgg atcgtcgtag tcggtgctcg cgaagtactc
     4141 catcggaacc ggattgctgc cgccgggcgg aagcagccgg ccttcgccga gcggtagcgt
     4201 gaagtagctc gcgtcgccgc cgaacgcgcc tgcgtcgacg ctggcggccc ccgagcacag
     4261 aatcccgacc gcgccgggcc gcggcagcgc gtggcgctga aaccatgcga ccgacatggc
     4321 cgtgagcatc ccgccggccg agcagccgta gaggccgatg ttctcgggcc ggtaggtctt
     4381 cgtgagctca cggtacgccg cggcgacgtc ctcgctggcc gccgggtggc gatgctgcgg
     4441 accctggcgg tagtcgaggc tcacgacctt gatccgcccg atcgccgcaa tcggcagcga
     4501 ctcgagctcc gcacaggcgg gccagcaccc gctaaagccg ccgccgtgca attggatcaa
     4561 cacgcgatcg cgattctcgg gcgcgatcct gtttcgcggc gtgtagacgt acgcgtgcac
     4621 gccgccgatc gttacgtctt cgcgctcgac cgggaacagc tcgcgctggc gcgcgatgaa
     4681 gccggcgagc agcggcggca cgccgtcttc ttgcacgaga agctccggcc gctgcaagtt
     4741 gcgcagatgc tcggcgagat acgccttgcc ttccggagac aggaagccgg aaaccggcac
     4801 gctctgggca ggcatgtgca cggtgccgtc ggcttcgacg agcacttgcc gcggcggggt
     4861 cggttgcggc cgtgctgctg tgcccagcag caggccgaga acgcccatcg ccactagacg
     4921 catggacgtc gagcgccgtt gcattgcgaa tctccagggg cgcgtcgagc ccgctacggt
     4981 cgcgaaaagt acctctgcct gcaatcgatt gcaaaccaac ccgcggctac tatgatcggg
     5041 taacgccatc gcgctcgacg tcggcattgc cgacacgctg cgcgccgaca acgtagaccc
     5101 cgccggggcc cgtaggctga aggtcttgcg cgctggccgc gagtcgggga ttctcttgcg
     5161 cccgagtcga agtcccaggc gccgcaggtt cgatggccga tttgcccgta ctcattgccg
     5221 gtggcggaat cggtggactc agcacggcgc tcacgttgca tcagctcggc gtgccgtgcc
     5281 tcgtcctcga ggcagtcacc agcctgaagc ccctaggtgt cggaatcaat ctacagccga
     5341 acgcggtgcg cgagctgtac gagctcggca tcggtcccgc ccaattgaac gcgatcggcg
     5401 tgcaggcgcg agagtgggcg ctcgtcggat tgaacggcaa agacgtgtat tccgagccgc
     5461 gcggtctgct cgccggttac cgttggccgc agtacgccgt gcatcgtggg gccttgcaga
     5521 tgctgctgca ccgcacggcg gtcgagcgcc tcggccccag cgcagtgcgc accgggcacc
     5581 gcgtcgccgg ttatcgtagc gacgcggacg gcgtcacggc gctgatcaaa actgcaaccg
     5641 gagcccagcg cgagcttcgg gggaagctgg tgatcggggc cgatggcctg cactcggctg
     5701 tgcgcgccca gatgcacccg cggcagccgc cgattcactg gggcggcgcc atcatgtggc
     5761 gcggtatgac cccgggcgta ccgatccgca gcggtgcatc gttcgtcggc ctcggcacgc
     5821 accgtcatcg cgtcgtgttc tctccgatct cacacgctga tccggcgacc gggcttgcga
     5881 tgatcaactg gatcgccgag atcaccgtcg acagctcgcg gggcctccgc gacggcgatt
     5941 ggaacaagaa ggtcgcgatc gactccttca ttcatcactt cgacggctgg cactacgact
     6001 ggctcgacgt gcccgcgatg ctgcgcggcg ccgaagaagt gttcgaatac ccgatgatcg
     6061 atcgcgaccc ggtgccgacc tgggtcgacg gccgtgtcgc gctgctcggc gacgcggccc
     6121 acgtgatgta cccgacgggc tcgaacggcg cgagccaagc gatcgtcgac gcgcgcgtgc
     6181 tcggtgcggc gatggtcgag cacggcgtta ccgccgaggc gctgcaggcg tacgaccgga
     6241 aactgtgcgc ggaaatttct gccgtcgtct tgcgtaaccg cggcgccggc cccttcgggc
     6301 tgctgaatct gctcgacgag cgctgcggtg gagttttcga cgagatcgat gccgtaatct
     6361 ccccgccgga gcgcgaggag ttcatgagcc gctaccgcgc cgcagccggt ttcgccaagg
     6421 accggctgaa cgccgcgccg ccgaccatcg cacccggcgc gagagtcgct gctacttaat
     6481 ccgccgcgca gccgaggatc gctgcgagac gcgcgctcac tgtctaattt ctgcgccgcg
     6541 cgcggacacg cgttcgaaga agtcgaagac cgccggccaa accatcggca ccatgccgcc
     6601 gtggtcgccg tcgacctcga ggtaatcgaa gtcgaagtcg cctctgcgca tgaactcggc
     6661 gagcgcccgg ctgccggcaa gggaaggcgt ggcgcccgtc ccttcggtca cgaagactgg
     6721 cagcttgcgg attcggtcga acgggtacga ctccttgtct ataccggggc ccgacatcgg
     6781 agccacggcg cgccagcgct ccggatagcg cgcggcgagg tgccagactc cgccgctgcc
     6841 catcgaatgt cctgcgagaa acgtgcgcga ccggtcggcg ccgtactctt cggtgacgat
     6901 ctctagcacg gtgatgacct cgagctcgct caaggcgagc tcacggcgac gttgcggcgt
     6961 gacggccgcg cgctgcgccg ctgcggttgc cgactcgccg aacacggcag gcagccgcag
     7021 cggattgccg taagcgccga gcggcgtgaa tccgagcggg gagacgacga catagccgtg
     7081 ctgctcggcg agcctgcgca gcaggccgtc cgctacgtcg agatacgtgc tctcgttcgc
     7141 gccggcgcca tgcaggaaca atacgatcgg cagcgagcgc tcgccgtccc atgtgctcgg
     7201 cacgtaaagg cgatagggca gctcggtgcc ggtcggcgca aatgcatagt ggcgatgctg
     7261 gtcgcccgta ggcggggcgg cgtgcgccgc gacggcggcg atgccgagcc acgcgagtgt
     7321 caagacgagc aagcagcgtt gcatcgacgt ctcctcgtta cgttgcgcgc cgcgtcatgc
     7381 gatacagcag cacgatcaag ccgagcgtca gcagatctgc gaccgcgacc atgtagaaac
     7441 ccgcggagaa cgagcccgta gcgtcgcgca agatgccgag catctgcggg ccgaaatagc
     7501 cgaacagccc ggcccccaag cttacgatgc ccatcgcgtt accggtttcg cggcgcggca
     7561 acaagctgcc tgcgaggccg taaagctggt tggcggcgga aaagccgaag ccgatcacga
     7621 ccgccgtcgc gatgcggaac gtgagctcgt tctcgagcgc caacgacgcc gacgccgtcg
     7681 cgagcacggc gacgatggcg gcgagcacga cgagcttgtc gaagcggtcg gtcagcgtgc
     7741 cgatcacgag attcaacacg atcgcagcaa tatagcccgt gctgatgatg atgccggccg
     7801 cggcagcgtc gagcccgtat acggaagcgg caacgctcgg cacgaagtaa gtaaccgtga
     7861 attggccgaa accgccaagt ccgacgacca gggcgagcag ccagacgacg ggcgagcgaa
     7921 acgcgctggg ttggccagct ccgtcggtcg cgacctcggg ctccgactcc ggcgctgcgc
     7981 tcgggcgata gaagaggaag aacacggccg cgccaagcaa cgccatggcc gcataaccca
     8041 agatcgcggt gcgccagccg agctcgccga cgagcgcgcc gccgagcggg gcgccgatga
     8101 cggacgccat cgctgacgtc gcgccgagca ctcccatcgt tcgcccgcgc agttgcggcg
     8161 gcgcagtcat cgccacggca atcagcacgc tgacgaaagc gaaccgatag ccgaagatcg
     8221 tcaggccgcg gaaggcgacc gccgactcga agctccacgc acgagcgagc aacacgctgc
     8281 caatcgcgac tccgacgagt cccgccgcga gcacgcgctt ctcgccgaag cgtttggccg
     8341 ccaagccggc gggtacgctc agcagcagcg cgagcaagcc gtaaagaccc gtgaagaagc
     8401 cgagctgtgt gaag
//
LOCUS       DATLEE010000569         3733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1408484, whole genome shotgun sequence.
ACCESSION   DATLEE010000569 DATLEE010000000
VERSION     DATLEE010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408484"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VM692_12330"
     CDS             <1..304
                     /locus_tag="VM692_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HUQ53003.1"
                     /translation="LEALDARIVDLHPPTRRHYYHPAMQGSWSIKAVLPTVAPDLSYG
                     ALGEVRDGLAAQSAYLEAIKPGTTDARRAALRRALLDYCRQDTLALVKLVEFFGRD"
     gene            340..1443
                     /locus_tag="VM692_12335"
     CDS             340..1443
                     /locus_tag="VM692_12335"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53004.1"
                     /translation="MVRSSALQVLAALAAIAVTPTGHAHHAFATEFDADLTGEVKGEV
                     TRVWWQNPHIRYDVAMKMPDGTTQSWALLPPGNLPTYRRENWSEQTVQVGYQVTATGN
                     LGRDGAKKLYATCINIGTGPEKGRQLGRCATAATVTQTTADPNVDYTVHANKYEVDIS
                     GYWDNRYKFQVTVDDFQPKPMPMTPATAALYKGRTYGDDHVLRCLPAGLPRIFGSPYP
                     MQIVDAGSHYLMVFMQDNTPRRVFMDGRAPPAEQPLTSMGFSTGKWEGRTLVIETTML
                     LPGWLDGSGYPMTGGADTRIVERWTPAADGLTMERVMTVYDTAYTQPLVRTRGSQRGD
                     ASVGLIESEPCDARPFLNELLERGELEQRLKPQ"
     gene            complement(1450..1806)
                     /locus_tag="VM692_12340"
     CDS             complement(1450..1806)
                     /locus_tag="VM692_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HUQ53005.1"
                     /translation="MSEALRALQQRLAAFAAARDWDQFHSPKNLAMALSVEAAELVEE
                     FQWLTEEQSRTLDPERHERVRLELADVLLYLLRLADKLGVDLMRAADDKIALNERKYP
                     AERVRGSARKYDEYES"
     gene            1819..>3733
                     /locus_tag="VM692_12345"
     CDS             1819..>3733
                     /locus_tag="VM692_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HUQ53006.1"
                     /translation="MFARVSHTQHGIYHRAMRLVAELLQLSATDLANHLGCVHLSQLD
                     LAVAERRAERPRRKDPIVELLSERGRQHEAAYLRHLRGQKLVVAEIRTAPGADSVEAT
                     VAAMRDGADVIYQAPLADERWHGIADFLRKVARPSKLGNWSYEVTDAKLATETRAGTI
                     LQLCVYSNLLARLQGAQPEYAHVVAPHHRLELDQFEPEPHRLADYEAYYRLVKRRLET
                     ALAVRGATTYPEPAQHCDVCNWWVQCNARRRSDDHLTFVAGISRLQIKELRTRLDVDT
                     LERLGGLAEVPKPTRGSREAMTRTRDQAAIQLKARRLQTRQHEVLPLGPEHGFLRLPK
                     PARGDLFLDLEGDRLALEGGREYLLGISDTRGGYTPLWATNPAEEKRAFEQIVDRILA
                     AFANDRSMHVYHFGAYEPTAFKRLSGRYATRETELDTILRAELFVDLHTIVKHSLRAS
                     VETYSLKDLEQFYGLVRQQDLRIATASRRALEWAIEMREDLGLTAAAPVASPQAKPQL
                     ELGLDSGPAPSRFAEHVAIVERYNRDDCVSTAALRDWLEQLRAEAERAAGVELPRPEL
                     NSGEASEQVAEAAEETQRVMAALLHEVPVDAAERSEQQQTRWLMAQLLEWHRREEKAA
                     WWEYFRLLDLPLED"
BASE COUNT          651 a         1282 c         1233 g          567 t
ORIGIN      
        1 gctcgaagcg ctggacgcgc gcatcgtcga tctgcatccg ccgacgcggc gccactatta
       61 ccatcccgcc atgcagggct cgtggtcgat caaggccgtc ctgccgacgg tcgcgccgga
      121 cttgagctac ggcgcgctcg gcgaagtgcg cgacgggctc gcggctcaaa gcgcctacct
      181 cgaggccatc aagcccggca cgacggacgc gcgccgcgcg gcgctgcgcc gggcgctgct
      241 cgactattgc cggcaagaca ccttggcgct cgtgaagctc gtggagttct tcggccggga
      301 ttagacttag cggtagaaca cgaccgctcg aggtttgcca tggttcgctc ctccgcgctg
      361 caagtcctcg ccgccctcgc ggccatcgcc gtaactccaa ccggtcacgc gcatcacgcc
      421 ttcgcgaccg agttcgacgc cgacctcacg ggcgaagtga agggcgaagt cacacgcgtc
      481 tggtggcaaa acccgcacat ccgctacgac gtcgcgatga agatgcctga cggcacgacc
      541 cagagctggg cgctgctgcc gcccggcaat ctgccgacct atcggcgcga aaactggagc
      601 gagcagaccg tgcaagtcgg ctatcaggtc acggcgaccg gcaatctcgg ccgcgacggc
      661 gcgaagaagc tttatgccac ttgcatcaac atcggcaccg gtcccgagaa aggccgccag
      721 ctcggccgct gtgcgactgc cgccacggtg acgcaaacga ccgcggatcc caacgtcgac
      781 tacacggtgc acgccaataa gtacgaggtc gacatctcgg gatactggga caaccgctac
      841 aagttccaag tcaccgtcga cgatttccag ccgaagccga tgccgatgac gccggccacg
      901 gccgcgctct acaaaggccg cacgtacggc gacgatcacg tgctgcgctg tctgccggcc
      961 gggctgccgc gcatcttcgg ctcgccgtac ccgatgcaga tcgtcgacgc gggctcgcac
     1021 tacttgatgg tgttcatgca ggacaacacg ccgcgccgcg tcttcatgga tggccgcgcg
     1081 ccgccggccg agcagccgct gacgtcgatg ggcttctcga cgggcaaatg ggaaggccgc
     1141 acgctcgtca tcgagacgac gatgctgctg cccggttggc tcgacgggtc cggttacccg
     1201 atgacgggcg gcgccgacac gcgcatcgtc gagcgttgga cacccgccgc ggacggcctg
     1261 acgatggaac gtgtcatgac cgtctacgac acggcgtata cgcaaccgct ggtccgcacg
     1321 cgcggctcgc aacgcggcga cgcctcggtc gggctcatcg agagcgagcc gtgcgacgcg
     1381 cggccgttct tgaacgagct gctcgagcgc ggcgagctcg agcagcgact gaagccgcag
     1441 tagccgtctc taggattcgt actcgtcgta cttacgcgcg ctgccgcgca cgcgctcggc
     1501 gggatacttg cgctcgttca gcgcgatctt gtcgtcggcc gcgcgcatga ggtcgacgcc
     1561 gagcttgtcg gcgagccgta gcaagtagag gagcacgtca gcgagctcga gccgcacgcg
     1621 ctcgtgccgc tcggggtcga gcgtacggct ctgctcctcg gtcagccact gaaattcctc
     1681 gacgagctcg gcggcctcta cggacagcgc catcgcgaga ttcttcggcg aatgaaattg
     1741 gtcccagtcg cgagccgccg cgaacgcggc aaggcgttgc tgcaacgcgc gtagcgcttc
     1801 cgacatggcg ctctcctcgt gttcgcgcga gtatcacaca cgcagcacgg catttatcat
     1861 cgcgccatgc gtctcgtcgc cgaattgctg cagctgtccg ccaccgatct agcaaatcac
     1921 ttgggctgcg tccacctgag ccagctcgac ctcgccgtgg ccgagagacg cgccgaacgg
     1981 ccgcggcgca aggatccgat cgtcgagctc ctgagcgaac gcggccggca gcacgaggcc
     2041 gcgtacttgc ggcatctgcg cgggcagaag ctcgtagtgg cggagattcg cacggcgccc
     2101 ggtgccgaca gcgtcgaggc gaccgtcgcc gcgatgcgcg acggcgcgga tgtcatctac
     2161 caagcgccgt tggcggacga gcgctggcat ggcattgcgg atttcctacg caaggtagcg
     2221 cggcccagca agctcggaaa ctggtcgtac gaggtcacgg acgcgaagct ggcgaccgag
     2281 acgcgcgctg gcacgattct tcagctctgc gtgtattcga acctgctcgc tcgcttgcaa
     2341 ggcgcgcagc ccgagtatgc gcacgtcgtc gccccgcatc atcggctcga gttggatcag
     2401 ttcgagccgg aaccgcaccg gctcgcggac tacgaagcgt actaccggct cgtgaagcgt
     2461 cggctcgaaa cggcgctcgc cgtgcggggc gccacgacgt atcccgagcc cgcgcagcac
     2521 tgcgacgtct gcaactggtg ggtgcagtgc aacgcgcggc ggcgctccga cgatcacctc
     2581 accttcgtgg ccggcatcag ccggctgcag atcaaagagc tgcgcacgcg cctcgacgtc
     2641 gacacgctcg agcggctcgg cgggctcgca gaggtgccga agccgacgcg cggctcgcgc
     2701 gaagccatga cgcgcacgcg cgaccaagcc gcgatccaac tgaaagcgcg gcgactgcag
     2761 acgcgtcagc acgaagtgct gccgctgggc cccgagcacg gctttttgcg gctgccaaag
     2821 cccgcgcgcg gcgatctgtt cctcgatctc gaaggcgacc ggctggcgct cgagggcgga
     2881 cgcgagtact tgctcggcat cagcgacact cgcggcggct atacgccgct gtgggctacg
     2941 aaccccgccg aggagaagcg cgcgttcgag cagatcgtcg atcgcatcct cgcggcgttc
     3001 gcgaacgacc ggtcgatgca cgtttatcac ttcggcgcct acgagccgac ggcgttcaag
     3061 cgcctgtccg gacgctacgc gacgcgcgag accgagctcg acacgatcct gcgcgccgag
     3121 ctgttcgtcg atctgcacac gatcgtgaag cactcgctgc gcgcgagcgt ggagacgtac
     3181 tcgctgaagg acctcgagca gttctacggc ctcgttcgcc agcaggactt gcgcatcgca
     3241 actgccagcc gccgcgccct cgagtgggca atcgagatgc gcgaggactt aggtctaacc
     3301 gccgcggcgc ctgttgcatc gccgcaggcg aagccgcagc tcgagctcgg gctcgatagc
     3361 ggtccagcgc cgagcaggtt cgcggagcac gtcgccatcg tcgaacgcta caaccgcgac
     3421 gattgcgtct cgaccgccgc gttgcgcgac tggctcgagc agctgcgcgc cgaagcggaa
     3481 cgggctgccg gcgtcgagct gccgcggccc gagctcaatt ccggcgaagc cagcgagcaa
     3541 gtcgccgagg cggccgagga gacgcagcgc gtgatggcgg cactgctgca cgaggtaccg
     3601 gtcgacgccg ccgagcgcag cgagcaacaa cagacgcgat ggctcatggc gcaactgctc
     3661 gagtggcatc gccgcgagga gaaggccgcc tggtgggagt acttccgcct gctcgacctg
     3721 ccgctcgagg act
//
LOCUS       DATLEE010000570         2710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_1409797, whole genome shotgun sequence.
ACCESSION   DATLEE010000570 DATLEE010000000
VERSION     DATLEE010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409797"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VM692_12350"
     CDS             <1..1194
                     /locus_tag="VM692_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HUQ53007.1"
                     /translation="NALAARHSPQGQILRDWYGRRPSDTARRAFLFGALAHILEMDDL
                     HRESVTHPGCAVVPAAWAIAVQQGSGGHDFLRAVLAGYEAVCRIGNAVGRAHYAVWHN
                     TSTCGPYGAAVAAAMLLELREEPTVWAMGNAGTQSAGLWQFLEEGAMSKHLHAGRAAE
                     AGVLAAELAARGFTGPAEILEGRKGFFVAACPDAAPERVLAEPGAAWQLRLTSIKPWP
                     SCRHTHPVVDAALELHGRVEADAIAEVRIETYQAALALCDAPAPTSEYEAKFSLQHCA
                     AAALSDGALTLNSFDGSARTRLAALAARSTPVAVSHYESAYPDRWGARVTLRTAAGET
                     LSADRPACKGDPESPLTPNELRAKANTLLAHGGFTKERATSLIDAVLALPHGGELPDV
                     AQLVS"
     gene            complement(1199..2524)
                     /locus_tag="VM692_12355"
     CDS             complement(1199..2524)
                     /locus_tag="VM692_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53008.1"
                     /translation="MPMQTRRAHLASLLGAGVGAALPGSLRAAVGDDIPFEGVGVNIR
                     HLSYSDQGGRPDGVQVMLHRQHLYVGHMFSDGVTVLDARDPRELKPLHFFSTAPNTRT
                     HHLQVAEGMMLLANGANIVRMQSYDSTRDYFENTLADSLTKRQPFRAGLSIFDLDRNP
                     ARPREIAFLEMPGIGINRLWWAGGRYAYVSAHFDGFTDHILCIVDLQNVTKPEIVSRW
                     WLPGMHRAGGETPTAPQGKRYALHHMIVSGDRGYAAWRDGGLTIHDVSDAAQPRLLSH
                     VNWSPPYPGGTHTALPLPQRKLVVVADESNADYCAKGLFYTWVVDARAEENPVPIATL
                     PTPTGLDFCAMGNFGPHNLHENRPGTFVSEETIFATYHNAGVRVFDIRDQYRPKEIAS
                     WLPPMPTKMLDPRPRVARNALTCDCYVTTNGVIYATDWNAGLHVLEYRG"
     gene            complement(2530..>2710)
                     /locus_tag="VM692_12360"
     CDS             complement(2530..>2710)
                     /locus_tag="VM692_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53009.1"
                     /translation="GGQPGRLGCSHGISTDQLGNLYLADCFYGRIQKFEPLPDADPDK
                     IAGQILRTWDTWKPN"
BASE COUNT          426 a          915 c          940 g          429 t
ORIGIN      
        1 aacgcgctgg ccgcacgcca ttcgccgcaa ggccagatcc tgcgcgactg gtacggccgc
       61 cgtccttccg acacggcgcg gcgcgcgttc ttgttcggcg ccctcgccca catcctcgag
      121 atggacgact tgcaccggga atccgtgacg catcccggct gcgccgtcgt gccggccgcc
      181 tgggccatcg ccgtgcagca aggaagcggt ggccatgatt tcttacgcgc cgtgctcgcg
      241 ggctacgagg cggtttgccg catcggcaat gccgtcggcc gcgcgcacta cgccgtctgg
      301 cacaacacgt cgacgtgcgg gccctacggc gcagccgtcg cggccgccat gttgctcgag
      361 ctgcgcgagg agccgacggt ctgggccatg gggaacgccg gcacgcagtc ggcaggtctt
      421 tggcagttcc tcgaggaagg cgccatgagc aagcacttgc acgcgggccg tgccgccgaa
      481 gccggggtgc tcgccgcgga gctcgcggcg cgcggcttca cggggcctgc ggaaatcctc
      541 gaaggccgca aaggcttctt cgtcgcggcc tgccccgatg ccgcgcccga gcgcgtgctg
      601 gcagagccgg gagccgcgtg gcagctccgg ctcacgtcga tcaagccgtg gccgtcgtgc
      661 cgccacacgc atccggtcgt cgatgctgcg ctcgagctgc acggccgcgt cgaggccgat
      721 gcaattgccg aagtccgcat cgagacgtac caagccgcgc ttgcgctctg cgacgcgccc
      781 gcgccgacgt cggaatacga ggcgaagttc tcgttgcagc attgcgcggc ggcggcgctc
      841 agcgacggcg ctttgacgct aaactccttc gacggctcgg cgcgcacacg gctcgcggcg
      901 ctcgcagcgc gcagcacgcc tgtcgccgtc tcgcactacg agtctgccta tcccgatcgc
      961 tggggcgcgc gcgtgacgct gcgcacggcg gccggggaga ccctgagcgc ggatagaccc
     1021 gcgtgcaaag gcgatcccga aagtcccctg acgccgaacg agctacgcgc caaagcgaac
     1081 acgctgctcg cgcacggcgg gttcacgaag gagcgcgcga cgagcctgat cgatgccgtg
     1141 ctcgcactac cgcacggcgg cgagctgccc gacgtcgcac agctcgtgag ctgacgcgtc
     1201 agccgcggta ttcgagcacg tgcagaccgg cgttccagtc ggtcgcgtag atgaccccgt
     1261 tcgtcgtcac gtagcaatcg caggtcaacg cgttgcgcgc gacgcgcggc cgcgggtcga
     1321 gcatcttcgt cggcatcggc ggcagccatg acgcgatctc tttgggccgg tactgatcgc
     1381 ggatgtcgaa cacgcgcacg ccggcattgt gatacgtcgc gaagatcgtc tcctcgctga
     1441 cgaacgtgcc ggggcggttc tcgtgcagat tgtgcgggcc gaagttgccc atggcgcaga
     1501 agtcgagccc tgtcggcgtc ggcagcgtcg cgatcggcac gggattctct tctgcgcgcg
     1561 cgtcgacgac ccacgtgtag aacaaaccct tcgcgcagta atcggcgttc gactcatcgg
     1621 cgacgacaac gagcttgcgt tgcggcaacg gcaacgccgt gtgcgtgccg ccgggatacg
     1681 gcggcgacca gttgacatgc gagagcagcc gcggctgcgc ggcgtcgctg acgtcgtgaa
     1741 tcgtgagccc gccgtcgcgc cacgcggcgt agccgcggtc gcccgacacg atcatgtgat
     1801 gcagcgcgta gcgtttgccc tgcggcgccg tcggcgtctc accgccggcg cgatgcatgc
     1861 cgggcagcca ccagcgcgac acgatttccg gtttggtcac gttctgcaaa tcgacgatgc
     1921 agagaatgtg gtccgtgaag ccgtcgaagt gcgccgatac gtaggcgtag cggccgccgg
     1981 cccaccagag gcgattgatg ccgatacccg gcatctcgag aaacgcaatc tcgcgcggcc
     2041 gggcggggtt tcggtcgagg tcgaagatcg acaagccggc gcgaaacggc tgccgcttcg
     2101 tcaacgaatc ggccagcgtg ttctcgaagt agtcgcgcgt gctgtcgtag gactgcatcc
     2161 gcacgatgtt cgcgccgttc gcgagcagca tcatgccttc ggcgacctgc aggtgatgcg
     2221 tgcgcgtgtt cggcgccgtc gagaagaagt gcaacggttt gagctcgcgc gggtcgcgtg
     2281 catcgagcac cgtgacgccg tcgctgaaca tgtggccgac gtagagatgc tgccggtgca
     2341 gcatgacctg cacgccgtcg gggcggccgc cctgatcgct gtacgacaaa tgacggatgt
     2401 tgacgccgac gccctcgaac gggatgtcgt cgccgacggc ggcacgcagc gaacctggca
     2461 gcgcggcgcc gacgccggcg ccgagcagcg aggccagatg cgcgcgccgc gtttgcatcg
     2521 gcatgcgcgt cagttcggct tccaggtgtc ccaggtccgc aagatctggc ccgcgatctt
     2581 gtcgggatcg gcgtccggca gcggctcgaa cttttgaatg cggccgtaga agcaatcggc
     2641 caggtacagg ttgccgagct ggtccgtcga gataccgtgc gagcagccga gccggcccgg
     2701 ctggccgccc
//
LOCUS       DATLEE010000571         5187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8531403, whole genome shotgun sequence.
ACCESSION   DATLEE010000571 DATLEE010000000
VERSION     DATLEE010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8531403"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VM692_12365"
     CDS             <1..246
                     /locus_tag="VM692_12365"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HUQ53010.1"
                     /translation="HSLLGFNLGVEIGQLAIVCALFPILYLVRRQWVYPKVILRYGAA
                     LLIVVSMYWFIERGFAVDLPAGAVFNAVRDRVIETVT"
     gene            268..1416
                     /locus_tag="VM692_12370"
     CDS             268..1416
                     /locus_tag="VM692_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006519928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUQ53011.1"
                     /translation="MDFDWSEEQIARKRRVIEFARANLSKDVARRDRDGEFPIDDWRR
                     CADFGILSYPVPSAYSGRDGVDFLSAMLAMEGLGYACADNGLTFALNAQMWTVQLPIV
                     QFGTDQQKRRYLPGLCGGTMIGAHAITEPDSGSDVFSMKASAKRVPGGYLLNGTKKFV
                     SLGPVADVALVFATLNADLGRWGVTAFLVDRTTPGYTAGPVAEKMGLRTVPIGELEFK
                     DCLIPEECRLGPEGAGTSLSTSSLEWERCCILASQLGSMERQLEEGVRYARDRRQFGQ
                     PIGKFQSVANRLVDMKLRLETSRLLLYKVAWLKQSGRSAMLEAALLKLHLAEAFVASS
                     LDAIRLHGGHGYMTVNEVERDLRDAVGGVIYAGTSDIQRNIVAALLGL"
     gene            1420..2988
                     /locus_tag="VM692_12375"
     CDS             1420..2988
                     /locus_tag="VM692_12375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HUQ53012.1"
                     /translation="MAPELLTELVDCNAEADRQAVGYLDETLSYAQLARRSSQLANVL
                     IDQGLKKGDRVGIYMNKGIDTAVAMYAAMKAGGAYVPIDPAAPTERLVSILKDCGLRH
                     LVSSSDRRRQLRALVGHDTEVECVIGVDAGDDLPFRCWSWAEVAKAEATPPRGAVRAD
                     DLAYIIYTSGSTGRPKGIMHSHRSGLSFAKWAASEYGLERDDRLANHAPLHFDLSIFD
                     YFAGAVAGATTVLVPEAYTKLPASYSQLLSDQRITVLFTVPFALIQLLTHGALDERDL
                     SRLRWIIFGGEPFPTRQLKSLMRKLRGSRFDNMYGPAEINGVTHFSPATPEDIGDTVP
                     IGPLSTLARSLVVDDDDRPLESGAAGELLVHTPTMMQGYWGRPDLDARAFYVHPDSGD
                     VYYRTGDLVERRPDGVFEFLGRKDRQIKVRGYRIELDEVEAILAAHEGVEEAAAFSLP
                     DGGGSQAVSAVVTLAKSIAKAPHSSEILAHAKAYLPWYALPSDLRIRESLPRTTTGKI
                     DRRLLREQAMRGGE"
     gene            2985..3251
                     /locus_tag="VM692_12380"
     CDS             2985..3251
                     /locus_tag="VM692_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HUQ53013.1"
                     /translation="MSVSDPVKEKLLGFVVDELLNGEPLASDDNLLGDGVVSSLGMMR
                     LVGFIEESYAIKVPPEHFIIENFQTVDAISTYLTARSSAVAKDG"
     gene            3244..>5187
                     /locus_tag="VM692_12385"
     CDS             3244..>5187
                     /locus_tag="VM692_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HUQ53014.1"
                     /translation="MDDTSRNEGDLLPLTRAQRLLWTGQQLAPRDPLYNMVLAFRIRG
                     PLEVPRFVAAFGALIARCDALRVVIEERDGVPRQRFLQQLRYELPVVDFADQRDPERA
                     RRDWIDVHRGRQFSTGSPMFESALLKAGDGDFVWYLNQHHVITDAWSVSVIYHKMRVL
                     YRAVAEDGVAAVPDMPSFRDYVLAENARAASPRRAKAAAYWQRRLGAPPAASAFYRAR
                     PRQPGGRTQRVPVAADSSRSARIEALLELAPFRAFTRDLGKFQIMATALFAYLFRATG
                     NRELTIGTPSHGRTNARSKELAGLLVELFPLSVEVDDDETFVSLYRKVAARTQELLMN
                     AEPGITSVENNRSFDVVLNYITASFGDFAGLRMESEWVHSGFGDRNHLLRMQVHDFDE
                     TAAINVDLDLNEDAFVGEERNWAARHLRILLDALLDDPGQAIELPELTDQDARSRRID
                     SVNGPAGEARVEVTVVEMLARQAAATPDAIALVCGDDTLTYRELDRCASQVAARLVPL
                     GAGPGVTIALAMRRSVAAVVAMLGVLKAGAAYVPVDPDYPQQRIDFIVEDSGALLVLA
                     VANDHVPPRAGAVQTLEIDDWVAPGAYRNADVTLRRVGPSDVAYVIYTSGSTGQPKGV
                     LVTHGGLANYVAWSRAYYLRGEVL"
BASE COUNT          902 a         1609 c         1711 g          965 t
ORIGIN      
        1 cactcgctgc tcggcttcaa tctcggcgtc gaaatcggtc agctcgccat cgtgtgcgcg
       61 ctgtttccga ttctctatct cgtccgacgc cagtgggtct acccgaaggt catcttgcgc
      121 tacggcgcag cgctgctgat cgtcgtctcg atgtactggt tcatcgagcg aggattcgcc
      181 gtggacttgc cagcgggcgc cgtattcaac gcagtgcgcg atcgtgtcat cgagaccgta
      241 acgtagacgg cgccacgggg actgctaatg gacttcgact ggtcggaaga gcagatcgcg
      301 cgcaagcggc gcgtcatcga gtttgcacgg gccaatctga gcaaagacgt agcgcgtcgc
      361 gaccgtgacg gcgaattccc gatcgacgac tggcgccgct gcgccgactt cggaatcctc
      421 agctaccccg tgccgtcggc atacagtggg cgcgacggcg tcgatttcct gagcgcgatg
      481 ctcgcgatgg aaggtctggg ctacgcgtgc gccgataacg gcttgacctt cgctttgaac
      541 gcgcaaatgt ggacagtcca actgccgatc gtgcagttcg gcacggacca gcagaagcgg
      601 cgttatctgc ccggcctctg tggcggcacg atgatcggcg cgcatgcgat caccgagccg
      661 gactccggct cagatgtctt tagcatgaag gcgtccgcga agcgcgtgcc gggtggctat
      721 ctcctgaacg ggacgaagaa gttcgtttcc ctggggccgg tcgccgacgt tgcgttggtc
      781 tttgccactc tgaacgccga tctcggacgc tggggtgtta cggctttcct cgtcgaccgc
      841 accacacccg gctatacggc cggtcccgtc gcggagaaga tgggcctgcg caccgtgccc
      901 attggcgagc tcgaattcaa agactgcttg attcccgagg agtgcaggct cggccccgag
      961 ggcgccggca cgagcctgtc gacgagctcg ctcgagtggg agcgatgctg catcctggcc
     1021 agccagctcg gctcgatgga gcggcaactc gaggagggag tacggtacgc gcgtgaccgc
     1081 cggcagttcg gtcagccgat cggcaagttt cagtccgtcg ccaatcgttt ggtggacatg
     1141 aagctgcggc tcgagacgtc gcggctgctg ctgtacaagg tcgcgtggct caagcaatcc
     1201 ggccggtcgg ccatgctcga ggccgccctg ctgaagctac accttgccga ggccttcgtt
     1261 gcctctagcc tcgatgcgat ccgcctgcac ggcggacacg gctatatgac cgtcaacgag
     1321 gtcgagcgcg atctgcgcga tgccgtgggc ggcgtcatct acgccggtac ctccgacatc
     1381 cagcgcaaca tagtcgcggc attgctcggg ctctgaatga tggcgccgga gctactcacc
     1441 gagctggttg actgcaacgc ggaggcggat cgccaggccg ttggctatct cgacgagacg
     1501 ctttcatacg cgcagctcgc gcgtcgctcg agccaactcg ccaatgtgtt gatcgatcag
     1561 gggctgaaga aaggagatcg cgtcggcatc tacatgaata agggaatcga cacggcggtc
     1621 gcaatgtatg cggccatgaa ggccggcggc gcgtatgtgc cgatcgatcc ggcggcgccg
     1681 acagagcgat tggtatcgat cctcaaggat tgcggcttgc gacatctcgt ctcgtcttcc
     1741 gacaggcgtc ggcagctgcg ggcgctcgtc gggcatgaca ccgaggtcga gtgcgtcatc
     1801 ggcgtcgatg ccggcgacga cctgccgttt cgttgttggt catgggctga agtcgcgaag
     1861 gccgaagcga cgccgccgcg gggagccgtc cgcgccgacg atctggccta catcatctac
     1921 acgtccggct cgacgggccg cccgaagggc atcatgcatt cacatcgcag cgggctcagt
     1981 ttcgcgaaat gggccgcgtc cgaatatggt cttgagcgcg acgatcggct ggcgaatcat
     2041 gcgccgctgc acttcgatct cagcatcttc gactacttcg cgggcgccgt ggccggggcg
     2101 actacggtgc tcgttccaga ggcgtacacg aagctgccgg caagttattc gcaactcctt
     2161 tcggaccaac gaatcaccgt cctcttcacc gtacccttcg cgctcattca actgctgact
     2221 cacggggcgc tggacgagcg cgacctcagc cggctgcggt ggatcatttt cggcggcgag
     2281 ccctttccga cgcggcagct caagtctctg atgcgcaagc tgcgggggtc gcgcttcgac
     2341 aacatgtacg ggccggccga gatcaacggt gtcacgcact tctctccggc gactcccgag
     2401 gacatcggcg ataccgtgcc gattgggccc ctcagcaccc ttgcgcggtc gctcgtggtc
     2461 gacgacgacg accgaccgct ggaatccggt gcagccggcg agctgctggt ccacacaccg
     2521 acgatgatgc agggctattg gggtcggccc gatctcgacg cgcgcgcgtt ttatgtgcat
     2581 ccagattcgg gcgacgtcta ctaccgcacc ggcgatctcg tcgagcgccg accggacggc
     2641 gtgttcgaat tcctggggcg caaagatcgg cagataaaag ttcgcgggta tcgaatcgag
     2701 ctcgacgaag tcgaagcgat cttggccgcg cacgaaggcg tcgaggaagc agcagcgttc
     2761 tcgcttcccg acggcggggg tagccaagcg gtcagcgcgg tggtgacgct cgcgaaatcg
     2821 atagcaaagg cgccgcattc cagcgagatc ctcgctcatg ccaaggccta cctgccgtgg
     2881 tatgcgctgc cgagcgactt gcgtattcga gaaagtctgc ctcgaacgac cacggggaag
     2941 atcgatcggc ggctgttgcg cgaacaagcg atgcgaggcg gcgaatgagc gtgtcggatc
     3001 ccgtcaagga gaagcttctc ggcttcgtcg tggacgagct gttgaacggg gagccattgg
     3061 cctccgacga caacctgctt ggcgacggcg tcgtgagctc gctcgggatg atgcgcctcg
     3121 tcggtttcat cgaggagagc tacgcgatca aggttccgcc ggagcacttc atcatcgaaa
     3181 actttcagac ggtcgatgcc attagtacct atctaacagc gcgatcgagc gcggtggcga
     3241 aggatggatg acaccagccg caacgaaggc gatctgctgc ctttgacgcg tgctcagcgc
     3301 ttgctgtgga cgggacagca gctcgccccg agagatcccc tctacaacat ggtcttggcc
     3361 ttccgcattc gaggcccgct cgaggtccca agattcgtgg ccgcgttcgg ggcgttgatc
     3421 gcccggtgcg atgcgcttcg agtcgtgatc gaagagaggg atggcgtgcc gcggcaacgc
     3481 tttctgcaac agttacgcta cgagcttccc gtcgtcgatt tcgccgacca gcgggatccc
     3541 gaacgggctc ggcgtgactg gatcgacgtc caccgcggca ggcagttcag cacgggtagc
     3601 ccgatgttcg agtcggccct gctcaaggcc ggcgacggcg acttcgtctg gtatctgaat
     3661 cagcatcatg tgatcacgga tgcgtggtcc gtgtcggtca tctaccacaa gatgcgcgtg
     3721 ctctatcggg cggtggccga agacggtgtc gccgcggtgc ccgacatgcc gagcttccgc
     3781 gactacgtgc tggctgaaaa cgcacgcgct gcatcgccga ggcgcgccaa ggcggcggct
     3841 tattggcagc gccggctcgg cgcgccgccg gcggcgagcg cgttctatcg ggcacggcct
     3901 cggcagccgg gcggccgaac gcagcgggtc cccgtagcgg ccgactccag tcggtcggcc
     3961 cgtatcgagg cgctcctcga actggcaccg tttcgcgcgt tcacacggga tctcggcaag
     4021 ttccagatca tggctacggc gctgttcgcg tatctgttcc gggctacggg caaccgcgag
     4081 ctcacgatcg gaacaccatc gcatggtagg acgaacgcgc gctccaaaga gctggcaggg
     4141 ttgttggtgg agctctttcc gctgagcgtg gaggtcgatg acgatgagac gttcgtgtcg
     4201 ctctatcgga aggtcgccgc gaggacgcag gagctattga tgaacgccga gcccggcatc
     4261 acgagcgtgg agaacaatcg ctcgtttgac gtcgtgctca actacatcac ggcgtccttc
     4321 ggcgatttcg ccgggttacg catggagtcg gagtgggtgc actccggttt cggcgaccgc
     4381 aatcatctgc tccggatgca ggtgcacgac ttcgacgaga cggctgcgat caacgtcgat
     4441 ctggatctca acgaggatgc tttcgtcggc gaagagcgca actgggcggc tcgccacctg
     4501 cgaatcctgc tcgacgcgct gctcgacgat cccgggcagg ccatcgagct gcccgagctc
     4561 accgaccaag atgcgcgttc gcggcggatc gatagcgtga acggccctgc cggcgaggca
     4621 cgtgtcgagg tgacggtagt cgagatgctg gcgcggcaag cggccgcgac gcccgacgct
     4681 attgcgctcg tgtgcggcga cgacacgctc acctaccgcg agctcgatcg gtgcgcgtcg
     4741 caagtcgccg cgcggctcgt gccactcggc gcagggcccg gagttacgat agcgctcgcg
     4801 atgcgccgaa gcgtcgccgc cgtcgtcgcg atgctcggcg ttctcaaagc cggtgccgcc
     4861 tacgtgccgg tagatcccga ttaccctcag caacgcatcg atttcatagt cgaggattca
     4921 ggcgcgctgc tcgtgctggc ggtcgcgaac gaccatgtcc cgccgcgcgc gggcgcagtg
     4981 cagacgctcg aaatcgacga ttgggtggca cccggtgcgt accgcaacgc ggacgtaacg
     5041 ttgcggcgcg tgggcccgtc ggatgttgcg tacgtgatct acacctcagg ctcgacgggc
     5101 caaccgaaag gtgtcttggt cacccacgga ggattggcga actatgtcgc atggagtcga
     5161 gcgtactacc tgcgcggcga ggtgctc
//
LOCUS       DATLEE010000572         3215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5875743, whole genome shotgun sequence.
ACCESSION   DATLEE010000572 DATLEE010000000
VERSION     DATLEE010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5875743"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VM692_12390"
     CDS             complement(<1..298)
                     /locus_tag="VM692_12390"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUQ53015.1"
                     /translation="MQFMMMHKTDAYYEGGGKPSKELIAGVGKMVGDMARAGKLKAGD
                     GLAPSSQGVRLKFAGGKRTLTPGPFVGGNELVAGFAVMRVRSLDEAVDWASKYAA"
     gene            complement(304..1083)
                     /locus_tag="VM692_12395"
     CDS             complement(304..1083)
                     /locus_tag="VM692_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ53016.1"
                     /translation="MSHVISKDGTRIAYERRGSGPALLLIDGAMCSRAFGPMCKIAEL
                     LEQDFTVYLYDRRGRGESGDTQPYAKAREVEDIEALIAAAGGSVYAVGLSSGAALALE
                     AAASGAPITKLAVYEPPFMVDDRELADVDHAGNLRALISSGERGAAVKYFMRLVRVPA
                     PIIFMMTLMRGMWRKMQAVAHTLPYDMTIMGNWQIPTRLAALRTPTLAMYGSKTQQRL
                     QRAIEELVKVLPDVRQQVLPGQTHNVGAAVLVPALVSYFKE"
     gene            complement(1095..1475)
                     /locus_tag="VM692_12400"
     CDS             complement(1095..1475)
                     /locus_tag="VM692_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HUQ53017.1"
                     /translation="MSNKTVIIGRTLSALAIVFMVFDVGIKLAQLPIVAQTMADLGYA
                     PDLGLTIGIVELVCLVLYVVPRTAVLGAILLTAVFGGAIATHVRVDSPLFSHVLFGVY
                     LGLLVWGGLYLRNPRLRGLIPAAA"
     gene            complement(1472..1888)
                     /locus_tag="VM692_12405"
     CDS             complement(1472..1888)
                     /locus_tag="VM692_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUQ53018.1"
                     /translation="MRFMIMVRATNDIEAGKSAPESLFAEMAAFHEQLAKAGVLLDAA
                     GLQPSAKGWRIRYVGDQRTLVDGPFAEAKELIAGYTLIETKTREEALEWARRYPNPAW
                     DRGVAEIEVRQLFDLEDFEPSPAIERFRDIGMGAQS"
     gene            complement(2036..2341)
                     /locus_tag="VM692_12410"
     CDS             complement(2036..2341)
                     /locus_tag="VM692_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUQ53019.1"
                     /translation="MAKPKHYNRLAKRYPEFTAAVEKLGDTVKQAGPLEPKVAELIQL
                     AAAAAVGSEGSVHSHTRRALRAGATADEINHAILLLTSTIGFPAVAAALSWVDDSEA"
     gene            complement(2358..>3215)
                     /locus_tag="VM692_12415"
     CDS             complement(2358..>3215)
                     /locus_tag="VM692_12415"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HUQ53020.1"
                     /translation="VDLFGPDSDVPGPDVNTGPQVMAWMLDTYVMHHRDFLPGVITGK
                     PIELGGSLGRQSATSRGLTVCVHKAAARLGVKLQGATAAVQGFGNVGSYAAKFLVQSG
                     VKVVAISDVDVAYRKSTGIDIDAAIAHVMRQGSLVGFKGAAPLRRKSQLLELPVDILV
                     PAALENQITTQNAPRVRARIIAEGANGPTTPGADAILERQGTFVIPDILCNAGGVTVS
                     YLEWVQNRMGYYWPEARVNEDLQRIMESAFDEVYATAQKRRVSMRIAAFMVAIRRVTE
                     ASELRGLYA"
BASE COUNT          521 a         1093 c         1053 g          548 t
ORIGIN      
        1 cggcagcgta cttcgacgcc cagtcgacgg cttcgtcgag cgagcgcacg cgcatgacgg
       61 cgaaacccgc gacgagctcg ttgccgccga caaacggccc cggcgtcaac gtgcgcttgc
      121 cgccggcgaa cttcaggcgc acgccttgtg agctcggcgc gaggccgtcg ccggccttga
      181 gcttcccggc gcgcgccatg tcgccgacca tcttgccgac gccggcaatc agctccttgc
      241 tcggcttgcc gccgccttcg tagtaagcgt cggtcttgtg catcatcatg aactgcatgt
      301 tcgctactcc ttgaaatagc tgacgagcgc cggcacgagc acggcggcgc cgacgttgtg
      361 cgtctggccc ggcagcacct gctgtcgcac gtcaggcaag actttgacga gctcctcgat
      421 cgcgcgctgc aaacgctgct gcgtcttgct gccgtacatc gcgagtgtcg gcgtccgtag
      481 cgccgcgagg cgcgtcggga tttgccagtt gcccatgatc gtcatgtcgt acggcagcgt
      541 gtgcgcgacg gcttgcatct tgcgccacat gccgcgcatt agcgtcatca tgaagatgat
      601 cggcgccggc actcgcacga gccgcatgaa gtacttgacc gccgcgccgc gctcgccgga
      661 cgagatcagc gcacgcaaat tgccggcgtg atcgacgtcg gcgagctcgc ggtcgtcgac
      721 catgaacggc ggctcgtaga cggcaagctt cgttatgggc gcgccgctcg cggccgcctc
      781 gagcgcgagc gccgcgcccg acgacaggcc gacggcgtag acgctgccgc cggccgccgc
      841 gatcaacgct tcgatgtcct cgacctcgcg cgccttcgca tacggctgcg tatcgccgct
      901 ctcaccacgg ccgcgtcgat cgtagagata gacggtgaag tcctgctcga gcagctcggc
      961 gatcttgcac atcggcccga acgcgcggct gcacattgca ccgtcgatca gcagcagcgc
     1021 cgggccgctg ccgcggcgtt catacgcgat gcgcgtgcca tccttcgaaa tcacgtggct
     1081 catgtcgctc tccttcaagc cgccgcgggg atcaggccgc gcaagcgtgg gttgcgcaga
     1141 tagagcccgc cccaaacgag aagcccgaga tagacgccga acaagacgtg gctgaacagc
     1201 ggcgagtcga cgcggacgtg ggtcgcgatg gcgccgccga acacggccgt cagcaagatc
     1261 gcgccgagca cggccgtgcg cggcacgacg tacagcacga ggcacacgag ctcgacgatg
     1321 ccgatcgtca accccaagtc cggcgcgtag ccgaggtccg ccatggtctg tgcaacgatc
     1381 ggcagctgcg cgagcttgat gccgacgtcg aacaccatga acacgatcgc cagcgcgctc
     1441 aacgtgcgcc cgatgatgac ggtcttgttg ctcatgactg cgcccccatg ccgatgtcgc
     1501 ggaaccgctc gatggccggg ctcggctcga agtcctcgag atcgaacagc tgacgcacct
     1561 cgatctcggc cacgccgcga tcccaagccg ggttcgggta gcgccgtgcc cactcgagcg
     1621 cttcctcgcg cgtcttcgtt tcaatcagcg tgtagccggc gatcagctcc ttcgcctcgg
     1681 cgaacgggcc gtcgacgagc gtccgctggt cgcccacgta gcggatgcgc cagcctttgg
     1741 cgctcggctg caacccggcg gcgtcgagca gcacgccggc tttcgccagc tgctcgtgaa
     1801 aggctgccat ctcggcgaac aggctctcgg gggccgattt gccggcctcg atgtcgttgg
     1861 tagctcgaac catgatcatg aatcgcatcg aaatctcctc gttctcgcga gccggtgggc
     1921 cggtaaggcc ctatctcgat cggctctata gacacgtcga acgaggaaac gcgaaatcga
     1981 cagcggctac gcgattcttg cgaccgatga cggcgacggc ccgtcggcag gtgaatcagg
     2041 cttcgctgtc gtcgacccag ctcaaggccg cggccacggc cggaaagccg atcgtgctcg
     2101 tcagcagcag gatggcgtgg ttgatctcgt cggccgtcgc gccggcgcgc aaagcgcgac
     2161 gcgtatggct gtgcacggaa ccctcggagc caaccgcggc cgcagccgcg agctgaatga
     2221 gctcggcgac cttcggctcg agcggcccgg cttgcttgac cgtgtcgccg agcttctcga
     2281 cggcggccgt gaattcggga tagcgcttcg cgaggcggtt gtagtgtttc ggtttggcca
     2341 cgaacgtctc ctagttgtca ggcgtagagg ccgcgcagct cggaggcctc cgtcacgcgg
     2401 cgaatcgcga ccatgaacgc cgcgatgcgc atgctgacgc ggcgcttctg cgccgtcgcg
     2461 tagacctcgt cgaatgcgga ttccatgatg cgctgcaaat cctcgttcac gcgcgcctcg
     2521 ggccagtagt agcccatgcg gttctgcacc cactcgagat acgacacggt gacgccgccg
     2581 gcattgcaga ggatgtcggg aatcacgaac gtgccctgcc gctcgagaat tgcgtcggcg
     2641 cccggcgtgg tcgggccgtt ggctccttcg gcgatgatgc gcgcgcgaac gcgcggcgcg
     2701 ttctgcgtcg tgatctggtt ctcgagcgcg gccggcacga ggatgtcgac cggcaactcg
     2761 agcaattgcg atttgcgccg cagcggcgcc gcgcctttga acccgacgag cgatccttgc
     2821 cgcatgacgt gagcgatcgc ggcgtcgatg tcgatgccgg tcgacttgcg gtaggccacg
     2881 tcgacgtcgc tgattgcgac gaccttgacg ccgctctgca ccaggaactt cgcagcatag
     2941 ctgccgacgt tgccgaatcc ctgcacggcc gccgttgcgc cttgcagctt gactccgagc
     3001 cgcgccgcgg ctttgtgcac gcagacagtg agcccgcgcg acgtcgcgct ctgccggccg
     3061 agcgagccgc cgagctcgat cggcttgccc gtgatgacgc cgggcaggaa gtcgcggtgg
     3121 tgcatgacgt aggtgtcgag catccaggcc atgacctgcg gccccgtatt gacgtcgggc
     3181 ccgggcacgt cgctgtcggg cccgaacaag tcgac
//
LOCUS       DATLEE010000573         8573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_673087, whole genome shotgun sequence.
ACCESSION   DATLEE010000573 DATLEE010000000
VERSION     DATLEE010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673087"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /gene="gidA"
                     /locus_tag="VM692_12420"
     CDS             <1..472
                     /gene="gidA"
                     /locus_tag="VM692_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402386.1"
                     /note="GidA; glucose-inhibited cell division protein A;
                     involved in the 5-carboxymethylaminomethyl modification
                     (mnm(5)s(2)U) of the wobble uridine base in some tRNAs;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HUQ53021.1"
                     /translation="HDVIVRPSDVPTGAFAEPLTRDAPAYALLRRPGIEYAQVTALDR
                     VGRSPELAALDAELVEQWTQSLSIEAHYAGYVERQSTEIERQKREAGTRLPQDFDYGV
                     VNGLSNELREKLTRIRPNDIGQAARISGMTPAAIGLLLVELKKRNRPRLAGAGR"
     gene            488..2191
                     /locus_tag="VM692_12425"
     CDS             488..2191
                     /locus_tag="VM692_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HUQ53022.1"
                     /translation="MAERAGVNRDPMSRAAARQLVWLAGVAIVCLVALLAALGWAATL
                     TGGGAAASQALDYESRTVTTSVRNEPPNLDTTRSIDVESYNIQHTVMEGLLRYDVNSN
                     LEPGVAERWELDGTRATFWLRSNSVWSDGKPVTAHDFVFAWRTAVDPKTASQYAFLIY
                     PVKNGAAINRGELPPEALGVRAAGDRVLEVELENPIAYFHKLTAAQTYYPIREDFYKS
                     RNGRYGADAGDLLYNGPFRITRWVHGASLRMEKNELYWDKDSIWLNVINIGYITPDAV
                     ARLNLYQDGRVADVDYLPGEALDQVLQQGWPLRKYNDGSMWFIELNFREGHLTRNFHL
                     RRALQLANDPSELVYKVLKTPSFTVAESLFPASIKGEHGLFRQEHPPPHVTTDLAAAR
                     AELELAKQELGLDTLPPLVLLADDTPGAITHSEYLQEYLRRNLGLEIRIDRQNFRQRL
                     EKQNNGEFDLTIKGWSADYDDPLSFADLFASWNLNNNGRYVNPALDEQVRIAQESLDQ
                     AERLRAFAEVQRILIEDAVIIMAYERGVLYVQNPRLKNVARRVIGPPTDYARAYITEE
                     P"
     gene            2198..3130
                     /locus_tag="VM692_12430"
     CDS             2198..3130
                     /locus_tag="VM692_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ53023.1"
                     /translation="MLAYILKRLAQGAVTVWFIATATFVAMHAVPGDPLSGEKLANAQ
                     IRANLEARYGLDQPLLDQYVTYLGGMLRGDFGLSYVQQNRRVNDIIREHFPVSATLGI
                     LAMAFATVGGVLWGALTAIYRNRLPDVVIMFLVIVGISVPSFVVAAVSQYALVNLNSS
                     FGTSLLPVAGWGTVWHMLVPALVLGLSTMAYLTRLMRSSMLDITSSDFVRTARAKGLP
                     PTRIFLRHQLRNAILPVITVLGPQIALITTGGFVVELVFAIPGLGRYFVEAVHQLDYT
                     VIMGTTTFYGAFLVFMVIVVDVLYSFIDPRVALE"
     gene            3127..4527
                     /locus_tag="VM692_12435"
     CDS             3127..4527
                     /locus_tag="VM692_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ53024.1"
                     /translation="MSAEIWQPTAEDFAKAEARGDAQRIVRPSISYWQDAWQRLKRNP
                     RALVSLAIVAALALFTIAGPWVWRVNPAQQDVDQISRAPGARGTGRIVEPYGPWAGVT
                     VDALPTSGSAVATAIRLAEPATTQAVRLVWQPAVNAAGYHVYRNISNPSAGRALGLPL
                     GDILDPHRVSFEDRFDLEAVPYWYSIVALDAAGLEADDYAVFEVEPALVISADEAVAR
                     GWIADASAARIGDPVELPYHPLGTDYLGRDMLSRLMHGARVSLFIGVVAPLVFVFFGI
                     VYGSAAGFAGGRTDQLLMRIADFVVALPFLLFMILFRIAFGVGPGESGVLPMLVALVL
                     LSWPSTARLVRGQVLQIRNEGYIGASRLLGAKTPYLIFRHMVPNTIGVILVTLTFEVP
                     RVIFIEAFLSFIGMGVAPPTPSWGSMSNEGIKTMLAHPHELLFPAVFISVAVLAFNLL
                     GDGLRDALDVRLRGRE"
     gene            4524..5570
                     /locus_tag="VM692_12440"
     CDS             4524..5570
                     /locus_tag="VM692_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ53025.1"
                     /translation="MSAHPVSQLAEPVLEVNDLAVEFRTHGGVVQAVRGASFSVAAGK
                     TLAIVGESGCGKSVTVQSIMGLIPMPPGRITRGTALFRGIDILKRKVIDGRDVRGSRI
                     GMIFQDPMSSLNPTMKVGDQIAETLIVHRGYSRKSAAAHAVELLQSVNIPEAERRAQQ
                     YPFAFSGGMLQRAMIAMAIACEPAVLIADEPTTALDVTIQAQILDLLKELQRDVGMAI
                     VLITHDLGVVARMADEVAVMYAGEIVERGTADDIFYSPGHPYTIGLRAAMPSRDTARA
                     GLKPIEGSPPDLFAPPPGCPYFARCPYAMQVCEPHHPPAFELSATQSARCWLHHENAP
                     PRAPLQRGGKQWHR"
     gene            5558..6538
                     /locus_tag="VM692_12445"
     CDS             5558..6538
                     /locus_tag="VM692_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ53026.1"
                     /translation="MAPLVEARGVTKHFPLARGQVVHAVDNVSLTIAEREVVGLVGES
                     GSGKSTFGRALVGLHTKTAGEVLFRGVRLPDQYGPKDFRRYATEIQMIFQDPYSSLNP
                     RMTVGEIIGEGLKLNGRGDAAQVRDQVGDWLARVGLERSHASRYPHEFSGGQRQRVGI
                     ARALILKPKFIVCDEPISALDVSVQAQIVRLLGELKASLGLTMLFIAHDLSMVRYVSD
                     RMAVMYLGSLVEIGPADEVYFRPKHPYTEILVASNPQADPRTERHRRAISIRGEIASP
                     INVHAGCRFAARCPKVMEVCRSVTPQLRTVADGDTRREVACHLFEPPKSA"
     gene            6752..7312
                     /locus_tag="VM692_12450"
     CDS             6752..7312
                     /locus_tag="VM692_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUQ53027.1"
                     /translation="MPITLEGNAERGAVVGETCKGCHGIPGYYNASPAYHVPKLGGQN
                     ADYLEVALQGYRRGTRGHATMQAQASSLTDQDIADVAAYFSTFEGSAQAGRTTASAAT
                     IEAGRAKAATCVACHGQEGVAAAPQWPNLAGQHENYLLQALGQYKNGGRVDMVMNPLI
                     APLDEQSIEELAAFFSSRPHLHDTAR"
     gene            7364..8002
                     /gene="rsmG"
                     /locus_tag="VM692_12455"
     CDS             7364..8002
                     /gene="rsmG"
                     /locus_tag="VM692_12455"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HUQ53028.1"
                     /translation="MPPADSAVSSTELRRRLGALGFAMTAAQGAALAAFVELLLKWNK
                     VYNLTGVRGADEVVDRHLVESFALRALLKGTNVADVGSGGGLPGVPLAIVEPERQFTL
                     IESRAKRVHFLRHVVAELKLANTDVAHGRAEDLPVSRPFDTVLARAVAPPAELLTICR
                     HLTASGSILLLLTATHLQDAFRGLAPDFLAQPVAKDGPKLRSSIVLLERINA"
     gene            8028..>8573
                     /locus_tag="VM692_12460"
     CDS             8028..>8573
                     /locus_tag="VM692_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUQ53029.1"
                     /translation="MGAVICVANQKGGVGKTTTCVNLAASLAATQRRVLLIDLDPQSN
                     ATMGCGIDKNALEYTICDVLLDDMAAADVLQPAPEGGFTVLPSNQDLTSAEVELVQKP
                     DREFRLRKALEPVASHYDYILIDCPPALNMLTVNALSAADFVLIPMQCEYFALEGLSA
                     LVATIDRIRATANPKLEIHGIL"
BASE COUNT         1374 a         2933 c         2818 g         1448 t
ORIGIN      
        1 gcacgacgtc atcgtccggc cgagcgacgt gccgaccggc gcgttcgcag agccgctgac
       61 gcgcgacgcg ccggcatacg cgctgttgcg ccgccccggt atcgagtacg cgcaggtgac
      121 ggcgctcgac cgcgtgggcc gctccccgga gctcgccgcg ctcgacgccg agctcgtgga
      181 gcagtggacc caaagcctgt cgatcgaggc gcactatgcg ggttacgtcg agcgccaaag
      241 cacggagatc gagcggcaga agcgcgaagc gggcacgcgc ttgccgcagg atttcgatta
      301 cggcgtcgtc aacgggctct cgaacgagct gcgcgagaag ctcacgcgca ttcgtcccaa
      361 tgacatcggc caggccgcgc gcatctcggg catgacgccg gccgcgatcg gcctactgtt
      421 ggtcgagctc aagaagcgca atcggccccg cctcgccggc gcgggccggt aggcgaggcg
      481 tcgcgacatg gcagagagag ctggtgtcaa ccgcgacccg atgagccgag ccgccgcgcg
      541 gcaactggtg tggctcgccg gcgtcgcgat cgtctgtctc gtcgcgctgc tcgccgcgct
      601 cggttgggcc gcgacgctga cgggcggcgg cgctgcggcc tcgcaagcgc tcgactacga
      661 gtcgcgcacc gtgacgacgt ccgtgcgcaa cgagccgccg aatctcgaca cgacgcgctc
      721 catcgacgtc gagagctaca acatccagca caccgtcatg gaagggttgc tgcgctacga
      781 cgtcaactcg aatctcgagc ctggcgtcgc cgagcgctgg gagctcgacg gcacgcgcgc
      841 gacgttctgg ctgcggtcga actccgtgtg gagcgacggc aagccggtca cggcccacga
      901 cttcgtgttc gcgtggcgca cggccgtcga tccgaagacg gcgtcgcagt acgcgtttct
      961 gatttatccc gtcaagaacg gcgccgcgat caaccgcggc gagctgccgc ccgaggcgct
     1021 cggcgtgcgc gcggccggcg atcgcgtgct cgaggtcgag ctcgagaatc caatcgcgta
     1081 cttccacaag ctgacggcag cacagacgta ttacccaatt cgcgaagact tctacaagag
     1141 ccgcaacggc cgttacggcg cggacgccgg cgacctgctc tacaacggcc cgttccgcat
     1201 cacgcgctgg gtgcacggcg cgagcctgcg catggagaag aacgagcttt attgggacaa
     1261 ggactcgatc tggctcaacg tgatcaacat cggctacatc acgcccgatg ccgtcgcgcg
     1321 gctgaatttg taccaggacg gccgagtggc cgacgtcgac tacttgcccg gcgaagcgct
     1381 cgatcaagtg ctgcagcagg gttggccgct acgcaagtac aacgacggca gcatgtggtt
     1441 catcgagctc aacttccgcg aaggtcattt gacgcgcaac ttccatttgc ggcgcgcgtt
     1501 gcagctcgcg aacgacccgt ccgagctcgt ctacaaggtg ctgaagacgc cgagcttcac
     1561 ggtagctgag tcgctgttcc ctgcgtcgat caaaggcgag cacgggctgt tccggcagga
     1621 acacccgccg ccgcacgtca cgacggacct cgctgcggca cgcgccgagc tcgagctcgc
     1681 caagcaagag ctcggtctcg acacgttacc gccgctcgtg ctgctcgccg acgacacgcc
     1741 gggcgcgatc acgcattcgg aatacctgca ggagtatttg cggcgcaatc tcggtctcga
     1801 gatccgcatc gatcggcaaa atttccgtca gcggctcgag aagcagaaca acggcgagtt
     1861 cgatctcacg atcaagggtt ggagcgccga ctacgacgac ccgctgtcgt tcgcggacct
     1921 gttcgcgtcg tggaatctca acaacaacgg ccgctacgtg aatccggcgc tcgacgagca
     1981 agtacgcatc gcgcaggaat cgctcgatca agccgagcgg ctgcgtgcgt tcgcggaggt
     2041 ccagcggatc ctgatcgagg atgcggtcat catcatggcc tacgagcgag gggtgctcta
     2101 tgtgcagaac ccgcgcttga agaatgtggc gcggcgagtc atcggtcccc cgacggacta
     2161 cgccagggcc tacatcaccg aggaacccta agccagcgtg ctcgcgtaca tcctcaaacg
     2221 gctcgcacag ggagccgtga ccgtttggtt catcgcgacc gcgacgttcg tcgcgatgca
     2281 cgccgttccc ggcgatccgt tgagcggtga gaagctcgcc aacgcgcaga ttcgcgccaa
     2341 cctcgaggcg cgctacggcc tcgaccagcc gctgctcgac cagtacgtga cttacctcgg
     2401 cggcatgctg cgcggcgact tcggactgtc gtacgtgcag cagaatcgcc gcgtcaacga
     2461 catcattcgc gagcacttcc ccgtctccgc gacgctcggc atcctcgcga tggcgttcgc
     2521 gacggtcggg ggcgtgctgt ggggcgcact gacggcgatc taccgcaacc gcttgccgga
     2581 cgtcgtgatc atgttcctcg tgatcgtcgg catctccgtg ccgagcttcg tcgtcgcggc
     2641 cgtgagccag tacgcgctcg tcaacttgaa ctcgtcgttc ggcacgtcgt tgttgccggt
     2701 cgcagggtgg gggaccgtgt ggcatatgct cgtgccggcg ctcgtgctcg gcttgagcac
     2761 gatggcgtac ctgacgcggc tcatgcgctc gtcgatgctc gacatcacgt cgtcggattt
     2821 cgtgcgcacg gcgcgcgcga agggcttacc gccgacgcgc attttcttgc ggcatcaact
     2881 gcgcaacgcc atcttgcccg tcatcacggt gctcgggccg cagatcgcgc tgatcacgac
     2941 gggcgggttc gtcgtcgagc tcgtgttcgc gatccctggc ctcggccgct acttcgtcga
     3001 ggccgtccac cagctcgatt acacggtcat catgggcacg acgacgttct acggcgcgtt
     3061 cctcgtgttc atggtcatcg tcgtcgacgt tctctatagc ttcatcgatc cgcgggtggc
     3121 cctcgaatga gcgctgaaat ctggcaaccg acggccgagg atttcgcgaa ggccgaggca
     3181 cgcggcgacg cgcagcgcat cgttcggccg tcgatctcgt actggcagga cgcttggcag
     3241 cgcttgaagc gcaatccgcg cgcgctggta tcgcttgcga tcgtggccgc cctggcgctg
     3301 ttcacgatcg cggggccgtg ggtctggcgc gtcaacccgg cacagcaaga cgtcgatcag
     3361 atcagccgcg cgccgggtgc gcgaggcacc ggccgcatcg tcgagccgta tgggccgtgg
     3421 gccggtgtga cggtcgacgc gctgccgact tcgggttccg cggtagcgac ggcgatccga
     3481 ctcgcggagc cggcgacgac gcaggccgtg cggctcgtct ggcagcccgc cgtgaacgcc
     3541 gccggctacc acgtctaccg caacatctcg aacccgtcgg ccggccgagc gctcgggctg
     3601 ccgctcggcg acatcctgga tccgcatcgc gtcagcttcg aggaccgctt cgacctagag
     3661 gccgtgccgt attggtattc gatcgtcgcg ctcgacgcgg cgggtctaga agccgacgac
     3721 tacgccgtgt tcgaggtcga gcccgcgctc gtcatcagcg ccgacgaagc cgtcgcgcgc
     3781 ggctggatcg ccgacgcgag cgccgcgcgc atcggcgacc ccgtggagct gccttaccac
     3841 ccgctcggca ccgactacct cgggcgcgac atgctctcgc gcttgatgca cggcgcgcgc
     3901 gtctcgctgt tcatcggcgt cgtcgcgccg ctcgtgttcg tgttcttcgg catcgtctac
     3961 ggcagcgccg cgggcttcgc cggcgggcgc acggaccagc tgctgatgcg catcgcggac
     4021 ttcgtcgtcg cgttgccgtt cctgttgttc atgatcctgt tccgcatcgc gttcggcgtc
     4081 ggccccggcg agagcggcgt gttgccgatg ctcgtcgcgc tcgtgttgct gagctggccg
     4141 agcacggcgc ggctcgtgcg cggccaggtg ctgcaaatcc gcaacgaagg ctacatcggc
     4201 gcgtcgcgtt tgcttggcgc caaaacgccc tacctaatat tccgccacat ggttccgaac
     4261 acgatcggcg tgatcctcgt gacgctgacg ttcgaggtgc cgcgcgtcat cttcatcgag
     4321 gcctttttgt cgttcatcgg catgggcgtc gcgccgccga cgccgtcgtg gggcagcatg
     4381 agcaacgagg gcatcaagac gatgctcgcg cacccgcacg agctcttgtt ccctgcggtg
     4441 ttcatcagcg tcgcggtgct cgcgttcaac ttgctaggcg acggcttgcg cgacgcgctc
     4501 gacgtgcgcc tcaggggccg cgaatgagcg cgcacccggt ttcacaactg gccgagcccg
     4561 tgctcgaggt caacgacctc gccgtcgagt tccgcacgca cggcggcgtc gtgcaggccg
     4621 tgcgcggcgc gagcttctcc gtcgcggccg gcaagacgct cgcgatcgtc ggcgagtcgg
     4681 gctgcggcaa atccgtaacc gtgcagagca tcatgggatt gattccgatg ccgccggggc
     4741 gcatcacgcg cggcacggcg ctgtttcgcg gcatcgacat cttgaagcgc aaggtcatcg
     4801 acggccgcga cgtgcgcggc agccgcatcg gcatgatttt ccaagacccg atgagctcgc
     4861 tgaatcccac gatgaaggtc ggcgaccaaa tcgcggaaac actcatcgtg caccgcggtt
     4921 actcgcgcaa gtcggccgcc gcgcacgccg tcgagctgct gcagtccgtg aacatccccg
     4981 aagccgagcg ccgcgcgcag cagtatccgt tcgcgttctc gggcggcatg ctgcagcgcg
     5041 ccatgatcgc gatggcgatc gcctgcgagc ccgcggtcct gatcgccgac gagccgacga
     5101 cggcgctcga cgtcacgatc caggcgcaga tcctcgatct cttgaaggag ctgcagcgcg
     5161 acgtcggcat ggcgatcgtg ctgatcacgc acgatctcgg cgtcgtcgcg cgcatggccg
     5221 acgaggtcgc cgtcatgtac gccggcgaga tcgtcgagcg cggaacggcc gacgacattt
     5281 tttattcgcc ggggcatccg tatacgatcg ggctcagggc cgcgatgccg agcagagaca
     5341 cggcgcgcgc cggcttgaag ccgatcgaag gctcgccgcc cgatctattc gcgccgccgc
     5401 cggggtgccc gtacttcgcg cgctgcccgt acgcgatgca ggtctgcgag ccgcaccacc
     5461 cgccggcctt cgagctatcg gctactcaaa gcgcacgctg ctggctgcat cacgaaaacg
     5521 cgccgccgcg ggcgccgctg cagcgaggcg gtaagcaatg gcaccgctag tcgaagcgcg
     5581 gggcgtcacg aagcatttcc cgcttgcgcg cgggcaggtc gtgcatgccg tcgacaacgt
     5641 ctcgctgacg atcgccgagc gcgaggtcgt cggcctcgtc ggcgaaagcg gctccggcaa
     5701 gtcgacgttc ggccgtgcgc tcgtcggcct gcacaccaag acggccggcg aggtgctgtt
     5761 ccgcggcgta cggttgccgg accaatacgg ccccaaggat ttccgccgct acgcgacgga
     5821 gatccagatg atcttccagg atccgtactc gagcctgaac ccgcgcatga cggtcggcga
     5881 gatcatcggc gaaggcttga aattgaacgg ccgcggcgat gcggcgcagg ttcgcgacca
     5941 ggtcggcgat tggctcgcac gcgtcgggct cgagcgcagt cacgcctcgc gctacccgca
     6001 cgaattctcg ggcggccaac gccagcgcgt cggcatcgcg cgcgcgctga tcctcaagcc
     6061 caagttcatc gtctgcgacg agccgatctc ggcgctcgac gtgtccgtgc aggcgcagat
     6121 cgtgcgcttg ctcggcgagc tcaaggcgtc gctggggctg acgatgctgt tcatcgctca
     6181 cgacctgtcg atggtgcggt acgtctccga tcgcatggcc gtcatgtact tggggtcgct
     6241 cgtcgagatc ggcccggccg acgaggtcta tttccggccg aagcacccgt acacggagat
     6301 tctcgtcgcg tcgaacccgc aagccgatcc acgcacggag cgccatcgtc gcgcgatctc
     6361 gatcaggggc gagattgcgt cgccgatcaa cgtgcacgcc ggttgccgct tcgcggcgcg
     6421 ctgcccgaaa gtcatggagg tatgccgcag cgtcacgccg caactgcgca cggtcgcgga
     6481 cggcgacact cggcgcgaag tggcctgcca cctgttcgag ccgccgaagt cggcctaagt
     6541 cgtcggtata agtccctata cgcccccggg cccattcggc tgccatcgcg gccacgtttt
     6601 ggctaagatg cgcgccgccg agaagggaat ctccgaggag ctcctgcccg tgtttcgtcc
     6661 gaccgccgtt gccgccgccg ttttgacctg cgctcttggc gccgccgcct tcgcgcaaag
     6721 cagcgccccg ccggctccgg ctgccccgct gatgccgatt acgctcgagg gcaatgccga
     6781 gcgcggcgcc gtggtcggcg agacctgcaa gggttgccac ggcattcccg gctattacaa
     6841 cgcgagcccg gcctaccacg tgccgaagct cggcgggcaa aacgccgatt acctcgaggt
     6901 cgcgctgcaa ggctatcgtc gcggcactcg cggccacgcc accatgcagg cgcaagcctc
     6961 gtcgctgacg gaccaggaca tcgccgacgt cgcggcctat ttctcgacgt tcgaaggctc
     7021 ggcccaagcc ggccgcacga cggcgtcggc cgcgacgatc gaggccggcc gcgccaaagc
     7081 ggcgacctgc gtcgcgtgcc acggtcagga aggcgtcgcc gccgcgccgc agtggccgaa
     7141 tcttgccggt cagcacgaga actacctgct gcaggcgctc ggccagtaca agaacggcgg
     7201 ccgcgtcgac atggtcatga atccgctgat cgcgccgctc gacgagcaga gcatcgaaga
     7261 gctcgcggcg tttttctcgt cgcgcccgca cctgcacgac accgcccgct gaccgcggcg
     7321 ccgatcgggc gccgccggct cgccgcgagg catactgccg cgcatgccgc ccgccgactc
     7381 cgccgtttcg agcaccgagc tgcgccgccg cctcggcgcg ctcggctttg ccatgacggc
     7441 cgcgcagggc gccgcgctcg cggcgttcgt cgagctgctg ctgaagtgga acaaggtcta
     7501 taacttgacg ggcgtacgcg gcgccgacga ggtcgtcgac cgccatctcg tcgagagctt
     7561 cgctttgcgc gcgctgttga aagggacgaa cgtcgccgac gtcggcagcg gcggcgggtt
     7621 gcccggcgtg ccgctcgcga tcgtcgagcc cgagcgccaa ttcacgctga tcgagagccg
     7681 cgcgaagcgc gtgcacttcc tgcgccacgt cgtcgccgag ctcaagctcg cgaacaccga
     7741 cgtcgcgcac ggccgcgccg aggacttacc tgtatcgcgc ccgtttgata ccgtgctggc
     7801 ccgcgccgtg gcgccgccgg ccgagttgct cacgatttgc cggcacctca cggcctcggg
     7861 cagcatcctg ctgttgttga cggccactca tttgcaggac gcgtttcgcg ggctcgcgcc
     7921 cgatttcctc gcgcagccgg tggccaagga cgggcctaaa ttgcggagct cgatcgtgtt
     7981 gttggagcgg atcaatgctt aaggccgcgg gcgtgacgag atagcgcatg ggtgccgtca
     8041 tctgcgtcgc gaatcagaag ggcggggtcg gcaagaccac gacctgcgtc aatctcgcgg
     8101 cctcgctggc cgcgacgcag cgccgcgtgc tcctgatcga cctggacccg cagtcgaacg
     8161 cgacgatggg ctgcggcatc gacaagaacg cgctcgagta caccatctgc gacgtgctgc
     8221 tcgacgacat ggccgcagcc gacgtgctcc agccggcgcc cgaaggcggg ttcacggtct
     8281 tgccgagcaa tcaagacctg acctcggccg aggtcgagct cgtgcagaag cccgatcgag
     8341 agtttcgttt gcgcaaggcg ctcgagcccg tcgcttcgca ctatgactac atcctcattg
     8401 attgcccgcc ggcgctgaac atgctgaccg tcaacgcgct gtcggccgcc gacttcgtgc
     8461 tgattccgat gcagtgcgag tacttcgcgc tcgaaggcct gagcgcgctc gtcgcgacga
     8521 tcgaccgcat ccgcgcgacg gcgaatccga agctcgagat ccacggaatt ttg
//
LOCUS       DATLEE010000574         4326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4370761, whole genome shotgun sequence.
ACCESSION   DATLEE010000574 DATLEE010000000
VERSION     DATLEE010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4370761"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VM692_12465"
     CDS             complement(<1..497)
                     /locus_tag="VM692_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53030.1"
                     /translation="MSWSKSSSAVSAWVLLVLSAAAAAQPPGPQPPRTPRASAQVDVT
                     GNWVAQITEDWRWRMITPPKGDFASVPLNALGRQAADAWDPAKDVTAGEQCRAFGAGG
                     LLRLPTRLRIAWADDATLKMESDAGEQERLLHFDRNAPADGAATWQGHSVAEWTGGPA
                     PGAFA"
     gene            642..1610
                     /locus_tag="VM692_12470"
     CDS             642..1610
                     /locus_tag="VM692_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ53031.1"
                     /translation="MIERQFAVALAAGVATSWVLAQAPEPKRDDAGTTAIVERARAAA
                     GNDWTAAVDFFCGTAGRANRADDPEIAPTRVFDNLYAVGRTGTVVWAMTTPAGILLID
                     AGYPDQLESMLLPQLRKAGLDPNDVKYVLVGHGHADHFGGASYFQQRGARVALSAADW
                     ELIESPPPAAPGAPPAAGLPPPKRDVVVADGEPIVFGGVTITPVLIPGHTPGALGFVF
                     EVADGGVRHTAALFGGSILLSNRISDDGLRQYAQSLERFAAVTSRLGADVEIQNHPLY
                     DGFEAKLARLAARRPGDAHPFVVGRDGYQRFLTVMRECANAQLARR"
     gene            complement(1612..2541)
                     /locus_tag="VM692_12475"
     CDS             complement(1612..2541)
                     /locus_tag="VM692_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53032.1"
                     /translation="MRSTSLSLTAAVATLLASAAFAQPPPGTAGGPGYPKVYDSSMPF
                     EPHDIAGIWTPNVVGFGGGGRCRDCGDRGFSLEFPMFTPAGQAAFDMNTPSYGRALDS
                     DDAKAHPEEHIGRRRAQPPALGNDLYQTCNPMGIPRAILYPDPEEFIVLPDRILQHFQ
                     WGYGLRTIWLDGRALPKPIEVDIPRWWGYAVGRWEGNTLVVTSTGYDERTWIDHFGYP
                     HSDEMVLEERYTRINFDTLELKMTLTDPKYYGEPWVAQTKRMHLIPRDFIQSSNWSGL
                     LEDLCAPVDEVDTFNRLIRDPAGTGRPAAPPTR"
     gene            complement(2549..2968)
                     /locus_tag="VM692_12480"
     CDS             complement(2549..2968)
                     /locus_tag="VM692_12480"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53033.1"
                     /translation="MKHSSIAGVIGALAAFVVLPAVAHHGTLISYDRDQQWTREVVVT
                     SFHYANPHPQLFFELTGDDGKAVAWSGEMLPNPAGLLRVGWTKARSLEALAPGKKVTV
                     TVAPARAGGPVGLVMKITNQEGADIVSDSVPFPQPAQ"
     gene            complement(3081..3605)
                     /locus_tag="VM692_12485"
                     /pseudo
     CDS             complement(3081..3605)
                     /locus_tag="VM692_12485"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(3621..3983)
                     /locus_tag="VM692_12490"
     CDS             complement(3621..3983)
                     /locus_tag="VM692_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HUQ53034.1"
                     /translation="MNLRQFDLNLLVALDVLLTERNVTRAGERLFLSQPAMSGILARL
                     RHAFQDELLVRVGRNLELTALAAELARPVRECVQQVDDLLNLRRPFVPESSPWSFRIA
                     ASDYVVFLMLGPLLKALT"
     gene            complement(4037..>4326)
                     /locus_tag="VM692_12495"
     CDS             complement(4037..>4326)
                     /locus_tag="VM692_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HUQ53035.1"
                     /translation="AHFEGQYDLKITRRWILQPQLELNWYGQADRARRLGSGLSSVEA
                     GLRLRYELRREVAPYVGVVRARRAGGTAASARAAGLDPDDSSLVAGIRLQF"
BASE COUNT          694 a         1488 c         1480 g          664 t
ORIGIN      
        1 aacgcgaacg cgccgggcgc cggtcctccg gtccactcgg cgacggaatg cccttgccac
       61 gtcgcggcac cgtcggccgg cgcgttgcgg tcgaagtgca gcagccgctc ctgctcgccg
      121 gcgtcgctct ccatcttcag agtggcatca tcggcccagg cgatccgcag ccgcgtcggc
      181 agccgcagca agccgccggc gccgaacgca cggcactgct caccggccgt tacgtccttc
      241 gcgggatccc aggcgtcggc ggcttgccgg ccgagcgcgt tcaacggcac ggacgcgaag
      301 tcgcctttcg gcggcgtgat catgcgccaa cgccagtctt cggtgatctg cgcgacccaa
      361 ttgcccgtga cgtcgacttg cgccgaggcg cgtggggttc gcggcggctg cgggcccggg
      421 ggctgcgcgg cggccgcggc cgacaacacg agcaacaccc acgcgctgac cgcgctcgag
      481 ctcttggacc acgacatcgc tcgcccctcc ggcatgctgg cgaggcgaac gctacgctgc
      541 gccgggacga cgctgcaatc acatctgcca ataggtgcaa tcggcgcgcc ggattttcgt
      601 tcggcgccga tttgtggtct cgtggcacgg ggaggatcgc catgatcgaa cggcaattcg
      661 cggtcgcgct cgctgccggc gtcgcgacgt catgggtgct cgcacaagcg ccggagccga
      721 agcgcgacga tgcgggcacg acggccatcg tcgagcgcgc ccgcgcggcc gccggtaatg
      781 actggaccgc ggcggtcgac ttcttctgcg gcaccgcggg ccgcgcgaat cgtgccgacg
      841 atcccgagat cgcgccaacg cgcgtgttcg acaacttgta tgcggtcggc cggaccggaa
      901 ccgtcgtctg ggccatgacg acaccggccg gaatactgct catcgacgcc ggctacccgg
      961 atcagctcga gtccatgttg ttgccgcagc tgcgcaaggc agggctcgat cccaacgacg
     1021 tcaagtacgt gctcgtcggc cacggccacg cggatcactt cggcggcgcg tcgtacttcc
     1081 agcagcgcgg agcgcgagtc gcgctgtcgg ccgccgattg ggagctcatc gagagcccgc
     1141 cgccagccgc gccgggcgcg ccgccggccg cagggctgcc gccgccgaaa cgcgacgtcg
     1201 tcgtcgccga cggcgagccg atcgtgttcg gcggcgtcac gatcacgccc gtgttgattc
     1261 cggggcacac tccgggcgcg ctcgggttcg tgttcgaagt cgctgacggg ggcgtgcgcc
     1321 acacggcggc gttgttcggc gggtcgatcc tgctatcgaa ccgcatctcc gacgacggct
     1381 tgcgccagta cgcgcaatcg ctcgaacgct tcgcagccgt gacgagccgg ctcggcgccg
     1441 acgtcgagat ccaaaaccat ccgctgtacg acgggttcga ggcgaagctc gcccggctcg
     1501 cagcgcggcg ccccggcgac gcacatccgt tcgtcgtcgg ccgcgacggc taccaacggt
     1561 tcctgaccgt gatgcgcgag tgcgccaacg cgcagctggc gcgccgctga gctaacgcgt
     1621 cggcggcgcc gcgggtcggc ccgtgcccgc cggatcgcga atcagccgat tgaacgtgtc
     1681 gacttcgtcg acgggcgcgc acagatcctc gagcaagccg ctccagttcg acgactgaat
     1741 gaagtcgcgc gggatcaggt gcatgcgctt cgtctgcgcg acccacggct ctccgtagta
     1801 cttcggatcc gtcagcgtca tcttgagctc gagcgtgtcg aagttgatgc gcgtgtagcg
     1861 ctcctcgagc accatctcgt cgctgtgcgg gtagccgaag tggtcgatcc acgtgcgttc
     1921 gtcgtagccc gtcgacgtca cgacgagcgt gttgccttcc cagcggccga cggcgtagcc
     1981 ccaccagcgc gggatgtcga cctcgatcgg cttcggcagc gcgcggccgt ccagccaaat
     2041 ggtccgcaaa ccatagcccc attggaaatg ctgcaggata cgatccggca gcacgatgaa
     2101 ttcctcgggg tccggataca ggatcgcgcg cggaatcccc atcgggttgc aggtttgata
     2161 gagatcgttg ccgagcgcgg gcggctgcgc gcgcctgcgg ccgatgtgct cctcgggatg
     2221 cgccttcgca tcgtcgctgt cgagcgcgcg gccgtacgac ggcgtgttca tgtcgaacgc
     2281 ggcctggccg gccggggtga acatgggaaa ttcgaggctg aagccgcgat cgccgcagtc
     2341 gcggcagcgt cctccgccgc cgaagccgac gacgttcggc gtccagatgc cggcgatgtc
     2401 gtgcggctcg aacggcatgc tcgagtcgta gaccttcggg tagccgggcc cgccggccgt
     2461 gcccggcggc ggctgcgcga acgcggcgct tgcgagcagg gttgcgaccg cggcggtcag
     2521 cgacagcgaa gtcgagcgca tggttcccct actgcgccgg ctgcggaaac ggcacggagt
     2581 ccgagacgat gtcggcgccc tcttgattcg tgatcttcat gacgaggccg accggtccgc
     2641 ccgcgcgcgc gggcgcgacc gtgaccgtga ctttcttgcc tggcgcgagc gcctcgagcg
     2701 accgggcttt cgtccagccg acgcgcagta ggcctgcggg attcggcagc atctccccgg
     2761 accatgcgac cgctttgccg tcgtcgcccg tgagctcgaa gaacagctgc ggatgcggat
     2821 tcgcgtagtg aaagctcgtg acgacgacct cccgcgtcca ttgctgatcg cgatcgtacg
     2881 agatcaaggt gccgtgatgc gcgaccgccg gcagcacgac gaaggccgcc aaagcaccga
     2941 tgaccccagc tattgacgag tgtttcacgg cggctccgag cacgaactat cgcgccatcg
     3001 ttcttcgcgc gagcggcggt tgggaaatgg tatcgaccga tacccggtat cggcgctcgc
     3061 gatacctatg cggcgtcgcg tcagagcgtc gccgcgacct cgacgaaccg cgtacgcagc
     3121 cacgaatgcg cggcgctggc cgtatagcgc gggttccaga tcagcttttc gcgcagcggc
     3181 ggcacgtcga acggcggctc gacgagccgg atgttcgcgg cttgctgcaa ccgctcaccg
     3241 agcctgcgcg gcaccagcga gacgagcgaa gtctcgcgca gcaagaacgg cgccgtcgcg
     3301 aagctctccg tggacgcgac gatgcggcgc tcgcacccga cttgcgcgag gtagttctcg
     3361 gcgaccgagc cgtgatcggg acccgcgagc cgaaagctca gatgcgggag cgcggagaac
     3421 tcgtcgagcg tgaagcgctc actgcgatgc gggtgtccgg accacaccgc acagacccag
     3481 ccgtcctcga acaacggcac ggacggcatg ttttgctcga cctcggtcgg catcaccgcg
     3541 aaatcgagct cccccgccgc gagccgttcg ccggccgtgg gctcgagcgc gaagaaccgc
     3601 acggaaacgt tcggcgcgag ctacgtgagc gccttcaaca gcggccccag catcaagaag
     3661 acgacgtagt cgctcgccgc gatgcggaac gaccaaggcg agctctcggg cacgaacggc
     3721 ctgcgcaggt tcagcaggtc gtcgacctgc tgcacgcact cgcgcacggg tcgcgcgagc
     3781 tccgcggcaa gcgccgtcag ctcgaggttg cggccgacgc gcacgagcag ctcgtcctga
     3841 aacgcgtggc gtagccgcgc caagattccg ctcatcgcgg gctggctcag gaatagccgt
     3901 tcaccggcgc gagtgacgtt gcgctcggtc agcagcacgt cgagcgcgac gagcaggttc
     3961 aagtcgaact ggcgcaggtt catggcgcac acgatagcac tcgccgagaa gtgccggccg
     4021 ggtggctcgg gcttggtcag aattgcagtc ggataccggc gaccaagctc gaatcgtcgg
     4081 ggtcgagccc agccgcgcgc gctgaggcgg ccgtgccgcc ggcccgccgc gcgcgaacca
     4141 cgccgacata gggcgcgact tcacgccgca gctcgtaacg caaacgcaag cccgcttcga
     4201 cgctcgacaa gccgctgccc aagcggcgcg cgcgatcggc ttgaccgtac caattcagct
     4261 cgagttgcgg ctgaaggatc cagcggcggg tgatcttgag gtcgtattgc ccctcgaagt
     4321 gcgcgg
//
LOCUS       DATLEE010000575         2193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5143545, whole genome shotgun sequence.
ACCESSION   DATLEE010000575 DATLEE010000000
VERSION     DATLEE010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5143545"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VM692_12500"
     CDS             <1..318
                     /locus_tag="VM692_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53036.1"
                     /translation="ARLGAIEATGQWLAPTPPAIARFDLQVALEAARQTIAAGGGNAI
                     DACFDSATYRASSTAERVFHAGCAPATSGGVPRFATFAFWDGIHPTAAAHAAIGAALG
                     ALF"
     gene            364..648
                     /locus_tag="VM692_12505"
     CDS             364..648
                     /locus_tag="VM692_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53037.1"
                     /translation="MLAPKPKARRFLEMTQFVLTLASACAVVDASRIQTAAPDRPGYH
                     TSVPQPRQAARDRSLGDLARVERKRREAEQHASRRGLELDHALEQRQLPA"
     gene            complement(622..1065)
                     /locus_tag="VM692_12510"
     CDS             complement(622..1065)
                     /locus_tag="VM692_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HUQ53038.1"
                     /translation="MESTQRQLQRSIAAAALSHVHDDLGAAAGGTALYTLADPRDLRA
                     PRYVGQTRAPRRRFAQHVRTARLWLRDETPWWVRSPKYRPLYEWIRALHRDEGRLPFM
                     WVVDWLEPTADARMAERAAIMRLLAQGAALLNVEARLLGGQLPLL"
     gene            1212..1676
                     /locus_tag="VM692_12515"
     CDS             1212..1676
                     /locus_tag="VM692_12515"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53039.1"
                     /translation="MKRTVLATLVGLGLAATAATAQQPPGNDRTNQVTFSQADKDGDG
                     TVNREEGNAINGFDFSRADTNSDRTLTRQEFDAAMARSTARGDGKEGPLTGDRTEHVT
                     FEQADKNKDGKVDRDEAEDIDGFNFSAADADDSRWVSPEEFRTAMAGSRPRG"
     gene            complement(1736..2002)
                     /locus_tag="VM692_12520"
     CDS             complement(1736..2002)
                     /locus_tag="VM692_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HUQ53040.1"
                     /translation="MATGWAREGAVQEQIDDTVKDGVARAKSRLPHGPSATHCDECGE
                     PIPEARRAALAGVRLCVACQQKADAEVQHFSGYNRRGSKDSQLR"
BASE COUNT          378 a          743 c          717 g          355 t
ORIGIN      
        1 gccagactcg gtgcgatcga ggctactggc cagtggctgg ccccgacgcc gccggcgatt
       61 gcgaggttcg acttacaggt ggcactcgag gccgcgcgac aaacgatcgc ggccggcggc
      121 ggcaatgcga tcgacgcgtg cttcgatagc gcgacctacc gagcgtcgtc gacggcggaa
      181 cgggtctttc acgccggctg cgcgccggcc acttccggcg gtgtgcctcg cttcgcgacg
      241 ttcgcgttct gggacggcat tcacccgacg gcggccgcgc atgccgcgat cggcgcggca
      301 ctgggcgcgc tcttttgacg ccgattcagg ttcgattcag gtcgcgagcc ctaggcttga
      361 tccatgctcg ctccgaaacc gaaagcacgg cgttttctcg aaatgaccca gttcgtgctg
      421 acccttgcga gcgcttgcgc cgtcgtggat gcgagccgta ttcagactgc tgcacccgat
      481 cgtcccggct accacacgag cgttccgcag ccgcggcagg cggcgcgcga ccgcagcctc
      541 ggcgacctcg cgcgcgtaga acgcaaacgc cgtgaggccg agcaacacgc gagccgccgc
      601 ggactcgagc tcgatcacgc cctagagcag cggcaactgc ccgcctaaga ggcgcgcttc
      661 gacgttcagc agcgccgctc cctgcgcaag cagtcgcatg atcgcggcgc gctcggccat
      721 gcgcgcgtcg gccgtcggct ccagccaatc cacgacccac atgaacggca agcgaccttc
      781 gtcgcgatgc aacgcgcgga tccattcgta gaggggccgg tacttcggcg aacgcaccca
      841 ccacggcgtc tcgtcgcgga gccacaagcg agccgtgcgc acgtgctgcg caaaacgccg
      901 ccgcggcgca cgcgtctgac ccacgtagcg cggcgcgcgt aagtctcgcg gatccgccag
      961 cgtgtacagg gccgtaccgc ccgctgccgc gccgagatcg tcatggacgt gcgacagcgc
     1021 cgcagccgcg atcgatctct gcaattggcg ttgcgtgctt tccatcgttt gggtcgagca
     1081 tcccggttct tcgtcgacta ctgtgcatcg atacagcggt ttagcgccac ctttgctggg
     1141 ctcggacgga ccgcgtgctg gcatatgtct tgcaaaacca ggctgtcaat cacggaagac
     1201 ggaggtctcc aatgaaacgg acagtactcg cgacgctcgt aggtctgggt ctcgccgcaa
     1261 cggcagcgac ggcgcaacag ccgcccggca acgatcgcac gaatcaagtc acgttttcgc
     1321 aagcagacaa ggatggggac ggcacggtta accgagagga aggcaacgcc atcaacgggt
     1381 tcgacttctc gcgtgccgac accaacagcg atcgcacact gacgcggcag gagttcgacg
     1441 cggccatggc acgctcgacg gcgcgcggtg acggcaaaga gggtccgctg acgggcgacc
     1501 gcaccgagca cgttacgttc gagcaagccg acaagaacaa ggacggcaag gtcgatcgtg
     1561 acgaggccga agacatcgac ggcttcaact tctcggccgc cgacgccgat gacagccgct
     1621 gggtcagccc tgaggaattc cgcacggcga tggccggctc ccggccgcgc ggctgatttt
     1681 ctttaggccg taacaaagag gaagccggcg ttggccggct tcctctcgtc gccattcaac
     1741 gaagctgact gtccttgctg ccgcggcggt tgtaaccgct gaaatgctgc acttcggcgt
     1801 cggctttttg ctgacacgcg acgcatagcc gcacgccggc gagagccgcg cgccgcgctt
     1861 cgggaatcgg ctcaccgcat tcgtcgcagt gcgtcgcgct cggcccgtgc ggcaggcggc
     1921 tctttgcacg cgcgacgccg tctttcacgg tgtcgtcgat ttgttcctgt acggcgcctt
     1981 cgcgcgccca acccgttgcc atggcagtgt cgatgaattg ggggtgtcca gctcagagac
     2041 acgttgcccg aaatggttcc cggtcgccag tcggccaaac cgacgccgcc cggctgcacc
     2101 gacgtgcgag gcgagcggcg cggcgcgagc tcagccgggg atctcgggct cggcgagctg
     2161 ggcgagcagc gtcagcgatt cctgccagcc gag
//
LOCUS       DATLEE010000576         2223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4386298, whole genome shotgun sequence.
ACCESSION   DATLEE010000576 DATLEE010000000
VERSION     DATLEE010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4386298"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2200)
                     /locus_tag="VM692_12525"
     CDS             complement(<1..2200)
                     /locus_tag="VM692_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUQ53041.1"
                     /translation="MADTLQNQSHRPWLELYRGVRPELSPTSDSALAMFRAMLARDGN
                     APLVHYFDKSVTAADCDAMSDALAVGLQQRGVETGDRVAIYLQNVPQVVIAVLAIWKC
                     GAVVVPCNPMLRDRELGKILRSSGCLALICQEDLYVDVARAALPTTAVQHTITTSPLE
                     FLDSGKPLPTMLAGVRRNPSPDTADMFEVIARHKGERPRAVDLTGDDVAFMVHTSGTT
                     GDAKGAMNTHRNVVFATSVYEAWIGLSRKDVILGLAPLFHVTGLIGHVTLAMLTGSPL
                     VLFYRFDVNEACRLAALHRATFTVSAITAYIALLNSDALGKYDLKSLTKLYTGGAPTP
                     ASVLEDWHGRTGVRIQTMYGLTEATSPTHMTPHGAIPPIDPHTGAMAVGVPVCNTHVK
                     VITDAGHEAAPREIGEFVIAGPQIVPGYWQKPEETAKSLTADGLRTGDVGFMDEQGWF
                     YLVDRSKDMIVASGFKVWPREVEEVLYQHPAVREAAVVGIPDAYRGETIKAVISLKPG
                     QKVSPDEIRAFARERMAAYKYPRVVEIIDDLPKTTSGKIMRRLLQPTAHATALPVQAE
                     IVDVSYPQLRVALEARAVLEAGSVWLRLSRGTLPLAKTATLYDRLRAMLGQLRDGRTF
                     VDRTAFLTANEAYHSAVIDLAENEHLSMGFRRLRLRDLFGAALKNTDVVAENAVYFHE
                     YLTDSLAAGDAANAIKAIMSWGKHSATGVRRALDLHGDVATQRDELRPGGI"
BASE COUNT          361 a          747 c          743 g          372 t
ORIGIN      
        1 cgatgccgcc cggccgcagc tcgtcgcgct gcgtcgcgac gtcgccgtgc agatcgagcg
       61 cgcggcgcac acccgtcgcc gaatgcttgc cccacgacat gatcgctttg atcgcgttcg
      121 cggcgtcgcc ggccgcgagc gagtccgtca gatactcgtg gaagtagacg gcgttctcgg
      181 caacgacgtc ggtgttcttc aacgccgcgc cgaacaggtc gcgcagccgc aagcgtcgga
      241 agcccatcga caggtgctcg ttctccgcga gatcgatgac ggccgagtga tacgcctcgt
      301 tggcagtgag gaacgcggtg cgatcgacga acgtccgccc gtcgcgcagc tgcccgagca
      361 tggcgcgcag gcgatcgtag agcgtcgcgg tcttcgcgag cggcaacgtg ccgcggctca
      421 ggcgcagcca cacggagccc gcttcgagca cggcgcgcgc ttcgagcgcc acgcgcagct
      481 gcgggtacga gacgtcgacg atttcggctt gcaccggcaa cgccgtcgcg tgcgccgtcg
      541 gctgcagcag ccgccgcatg atcttgccgc tcgtcgtttt cggcaggtcg tcgatgattt
      601 cgacgacgcg cggatacttg tatgcggcca tgcgctcgcg cgcgaacgcg cggatctcgt
      661 cgggcgatac cttctggccg ggcttcaggc tgatgacggc cttgatcgtc tcgccgcggt
      721 acgcatccgg aatgccgacg acggccgcct cgcgcacggc tggatgctga tacagcactt
      781 cctcgacctc gcgcggccaa accttgaacc ccgacgccac gatcatgtcc ttcgagcggt
      841 cgacgagata gaaccacccc tgctcgtcca tgaaaccgac gtcgccggtc cggagcccgt
      901 cggccgtcag cgacttcgcc gtctcctcgg gcttctgcca atagccgggg acgatttgcg
      961 ggccggcgat gacgaactcg ccgatctcgc gcggcgcggc ctcgtggccg gcgtccgtga
     1021 tgaccttgac gtgcgtgttg cagaccggga caccgactgc catcgcgccc gtgtgcggat
     1081 cgatcggcgg gattgcgccg tgcggcgtca tgtgcgtcgg cgaagtcgct tcggtcaggc
     1141 cgtacatcgt ctggatgcgc acgcccgtgc ggccgtgcca gtcctcgagc acgctggccg
     1201 gcgtcggcgc gccgcccgtg tagagtttcg tcagtgactt gagatcgtat ttgccgagcg
     1261 cgtcgctgtt cagcagcgcg atgtaggccg tgatcgcgga gaccgtgaaa gtcgcgcggt
     1321 gcaaggcggc caaacggcac gcctcgttga cgtcgaaccg gtaaaacaaa accagcgggc
     1381 tgcccgtcag catcgcgagc gtgacgtggc cgatgaggcc cgtgacgtgg aacagcggcg
     1441 ccaacccgag gatgacgtcc ttgcgcgaca agccgatcca cgcctcgtac accgaggtcg
     1501 cgaacacgac gttgcgatgt gtgttcatcg cgcctttcgc gtcacccgtc gtgccggacg
     1561 tgtggaccat gaacgcgacg tcgtcgcccg tgaggtcgac ggcgcgcggc cgctcgccct
     1621 tgtgccgagc gatgacctcg aacatgtcgg ccgtgtccgg cgacggattg cggcgcacac
     1681 cggccagcat cgtcggcagc ggcttaccgg aatcgaggaa ctcgagcggc gatgtcgtga
     1741 tcgtgtgctg cacggccgtc gtcggcagcg ccgcgcgagc gacgtcgacg taaagatcct
     1801 cttggcagat caacgcgaga cagcccgagc tgcgcaggat cttgccgagc tcgcgatcgc
     1861 gcagcatcgg gttgcacggt acgacgacgg cgccacactt ccagatcgcg agcacggcaa
     1921 tgacgacctg cggaacattc tgcagataaa tcgccacgcg atcgccggtc tcgacgccgc
     1981 gctgctgcaa cccgacggcc agcgcgtccg acatggcgtc gcagtcggcg gccgtcaccg
     2041 acttatcgaa gtagtggacc aacggtgcgt tgccgtcgcg agcgagcatc gcgcgaaaca
     2101 tggcgagcgc cgaatcgctc gtcgggctca gctccggacg cacgcctcgg tacagctcga
     2161 gccaagggcg atgcgattga ttctgtaggg tgtctgccat tgctcgcagt gcgctcagtc
     2221 ctc
//
LOCUS       DATLEE010000577         6412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6302033, whole genome shotgun sequence.
ACCESSION   DATLEE010000577 DATLEE010000000
VERSION     DATLEE010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6302033"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1702)
                     /locus_tag="VM692_12530"
     CDS             complement(230..1702)
                     /locus_tag="VM692_12530"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HUQ53042.1"
                     /translation="MLFNSVTFVAFYVTVFAAYWLLHARRPQNLLLLGASWIFYGAWS
                     WKFLLLLLASTVLDYVCGLLIDGAASQRRKRLVLIVSVTSNLLFLATFKYLGFFAAEL
                     AALLERVGFDASLPVLNIVLPVGISFYTFQTIGYVVDVYRGKVRAERDFFDYALYVAF
                     FPQLVAGPIERAGHMLPQYKAPRVWSAPKFESGLQLAFWGLFKKVVIADKLAPYVDVV
                     YGNPADFSGVALVTATVFFAFQIYCDFSGYTDTARGVARMLGFELMPNFAFPYISKSP
                     VEFWQRWHISLSKWFQDYLYYPLAIRYMRKGGWGSKYKAHIVAMALIGIWHGANWTFL
                     VFGLYWGCVIALYLFLQERAANAEPGGLAERIAGWTAPVRDFGSVALMFMLVSIGWVY
                     FRAESIHDAWYVLTHVFSASGAPDVMRPEIVDTAVLWTLIAGLCCAEWLYRHSDLVRR
                     RLEANQLAAIAGRYALLAAIVVASGASQVDGARPFIYFQF"
     gene            complement(1709..2809)
                     /locus_tag="VM692_12535"
     CDS             complement(1709..2809)
                     /locus_tag="VM692_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HUQ53043.1"
                     /translation="MSEPVAVRVPQINPNDEHAIVVRWHVDKGTHVHAGQSVVTLETT
                     KTTFDVDAPQDGYLFFEHEPHSEVAVGAPVAWIADTAEPPQLRKEEPPRRADVPAAAP
                     SGRFTRKALKLMKERHLTAADFPGDERIDADAVERAAAARGTHGTHGVAQRPLDAEPL
                     ELRPSKLTEIQTLSRVHEQVVPSSVTVAVDCAALDERLRRLSEAHGPVSLLELCIYET
                     AQLLPEWPELNGFYADRKAWRYRSAAIGFAVNAGRGLRVPVIKGAGKLALHEVARAAR
                     ELSLRYMRDELAIDDVTGGTFTITDLSGEGVVHFMPVLNERQSSILGLTAERSVARTR
                     DLVLAFDHRLSDGMQAGRFLAALRDRLEGAEG"
     gene            complement(2802..4727)
                     /locus_tag="VM692_12540"
     CDS             complement(2802..4727)
                     /locus_tag="VM692_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUQ53044.1"
                     /translation="MNVERHIFDKAYLVRTVEEQLLELFAAGKLSGTTHTCIGQEMSA
                     VALAAALDRRKDVIVSNHRCHGHYLAWTDDPDGLVAEVMGKSTGVCAGLGGSQHLSAE
                     GFMSNGIQGGIVPVGAGLAFAKKLAGDGGIVAVCIGDGTLGEGVVYETFNMAAKWRLP
                     LLVVLENNRYAQSTSQAETLAGDICARAAAFGMRTLHGETWRPYVLAAQLREAAAAVR
                     HDGGPLFVRVDTYRLAAHSKGDDDRDPKEVAAFARRDPLNVFLAQQQVDADVGLAEIR
                     ERVARAIERAAAAPVLSAHESADVRLSIEWRAAALEPGTQLVAINRALQEWLEDDARV
                     LLLGEDIRSPYGGAFKATRGLSDAHPERVINTPISEAGIVGIANGLALAGRRPVVEIM
                     FGDFLGLCFDQLLNHAAKFRGMYNGRVSSAIVVRTPMGGGRGYGPTHSQNIEKHFAGM
                     PGLRVAVLHGRASIAALYAALRDSVEPTLIIENKLLYRESAAAALPPGYEAFETTGPW
                     PTTVLRPAGAPDLTIVAFGRMSLVAEQVAAELRAEEIEVEILLPLSVSPFDARPVVES
                     VARTRKLVVIEEGAAHFDLASEVIAAAAEACRGGGLHARRVAARPRPIPSALKLELEV
                     LPSVAALRSACLELYDE"
     gene            complement(4724..4963)
                     /locus_tag="VM692_12545"
     CDS             complement(4724..4963)
                     /locus_tag="VM692_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HUQ53045.1"
                     /translation="MSTTDIAARVETVFGRVFGKRMPFAPQLSRLDEPRWNSMKHMEF
                     LLGLEQEFGIRFDGADATDMVSMPDVVARVGSKLQ"
     gene            complement(4960..>6412)
                     /locus_tag="VM692_12550"
     CDS             complement(4960..>6412)
                     /locus_tag="VM692_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53046.1"
                     /translation="SFFLDRSTTCNMLSFSAKVDEPTTVRLWRLLVMVAGAGALAAAV
                     YRVPAWLAIWTPGFVAATVAAAAALLVLAFAVALQGSARAVERVALEIVFLTFALMAG
                     EAILLGRSPELWSDDSLAQRVIARERAALRQGVDFDERLRPDVIDELRARGLDAVSGV
                     AQTAGTTGVVASAVLERGLLPLSNASNAYVVECNEGNGYFQFRSDDLGFNNPPGLAHG
                     PIDIAVVGESFALGHCVAPSRSAVELLRARHPRTANFGVAGSRVLSQLGVMREYVQPL
                     RPPLVLWFVNTNFAEPRQEADQPLLTSYLDDPAYSQHLRERAHEVDAFIREVLVPLNR
                     SGAEELREELDVASRFPLERLFKMKELRSLLDFGPAARRAPPAPDLSHFQRALEQASS
                     SVQAWGGRLVVVILPSYEISTGLKLNNARYEAVRNIVAASTAGVVDGVALFAAQPDYA
                     SLYTLRIDNHPNAQGHALIAAALLTEVEHEEGK"
BASE COUNT         1160 a         2178 c         2087 g          987 t
ORIGIN      
        1 cccgtcgcga cgacgcgtga tgtgaaagtt tcgggcgggc tcgccgatac ggcgcgcggg
       61 ttgcgcggcg cggcggccgc cgtttgcgcg ccggccggcg aagcgccgaa cagaaccgcg
      121 ccgatgccga cagcaacgat cgctttggcg agaaccggtg tacgcatttg aggtcgccct
      181 tcgcaagccg cgtcgcgagc gattatgcga caaccggaaa tcggcggcct cagaattgga
      241 agtagataaa cggtcgcgcg ccgtcgacct gtgatgcgcc gctcgctacc acgatcgcgg
      301 ccagcagcgc atagcggccc gcgatcgccg ccagctggtt ggcctccagc cggcgacgca
      361 cgaggtccga gtgccggtag agccattcgg cgcaacacaa accggcgatc agcgtccata
      421 gcaccgctgt gtcgacgatc tccggccgca tcacgtccgg cgcgccgctc gccgagaaca
      481 catgcgtgag cacgtaccaa gcgtcgtgga tcgactccgc ccggaaatag acccagccga
      541 tcgagaccaa catgaacatc agcgccacgc tgccgaagtc gcgcacgggg gcagtccagc
      601 cggcgatgcg ctccgcgaga ccaccgggct ccgcgttcgc cgcgcgctcc tgaagaaaga
      661 gatacagcgc gatgacgcag ccccaataga ggccgaaaac gaggaacgtc cagttcgcgc
      721 catgccagat tccgatgagc gccatggcaa cgatgtgggc tttgtacttg ctgccccagc
      781 cgcctttgcg catgtaacgg atcgccagcg ggtagtacag gtaatcctgg aaccacttcg
      841 acaggctgat gtgccagcgc tgccagaact cgacgggact cttcgagatg tacgggaaag
      901 cgaagttcgg catcagctcg aagccgagca tgcgtgcaac gcctcgcgcc gtatccgtgt
      961 agcccgagaa atcgcaatag atctggaacg cgaagaacac cgtcgcggtg acgagcgcga
     1021 cgccggagaa gtccgccggg ttgccataga cgacgtcgac gtagggcgca agcttgtcgg
     1081 caatgacgac cttcttgaac aagccccaaa acgcgagctg caagcccgac tcgaacttcg
     1141 gcgcgctcca aacgcgcggc gccttatatt gcggcagcat gtgtcccgcg cgttcgatgg
     1201 gccccgccac gagctgcggg aagaacgcca cgtacagcgc gtaatcgaaa aaatcgcgct
     1261 cggcacgcac cttgccgcgg tagacgtcga cgacgtagcc gatcgtctgg aacgtgtaga
     1321 acgagatgcc gacgggcaga acgatgttga gcacgggcag gctcgcgtcg aagccgacgc
     1381 gctcgagcag cgccgcaagc tcggctgcga agaagccgag atacttgaac gtcgcgagaa
     1441 acagcaggtt gctggtgacg ctgacgatca gcacgaggcg cttgcggcgc tggctcgccg
     1501 cgccgtcgat cagcagaccg cagacgtaat cgagcaccgt gctcgcgagc agcagcaaca
     1561 ggaacttcca gctccacgca ccgtagaaga tccagctcgc cccgagcagc aataagtttt
     1621 gcggtctgcg cgcgtgcagc agccaataag ccgcgaaaac cgtgacatag aacgcgacga
     1681 atgtgaccga gttgaacagc attagccgtt acccctcggc gccttcgagc cgatcgcgca
     1741 gcgccgcgag gaatcgcccg gcctgcatgc cgtccgacag ccggtgatcg aacgcgagca
     1801 cgagatcgcg cgtgcgtgcg acgctgcgct ccgccgttag gccgagaatg gacgactggc
     1861 gctcgttgag caccggcatg aagtgcacga cgccttcgcc ggacaggtcc gtgatcgtga
     1921 acgtgccgcc cgtgacgtcg tcgatcgcaa gctcgtcgcg catgtagcgc agcgacagct
     1981 cgcgcgcggc gcgcgccact tcgtgcaacg ccagtttgcc cgcgcctttg atgaccggaa
     2041 cacgcaggcc gcgcccggca ttgaccgcga agccgatcgc cgcggatcga tagcgccacg
     2101 ccttgcggtc ggcatagaag ccgttgagct ccggccattc gggcagcagt tgcgccgtct
     2161 cgtaaatgca gagctcgagc aagctgacgg ggccgtgcgc ctccgagagg cggcgcagac
     2221 gttcatcgag agccgcgcaa tcgactgcga cggtgaccga gctcggcacg acttgctcat
     2281 ggacgcggct tagcgtttgg atctccgtga gcttcgacgg ccgcagctcg agcggctccg
     2341 cgtcgagcgg ccgctgcgcg acgccatgcg tgccgtgcgt gccgcgcgcc gccgcggctc
     2401 gctcgacggc gtcggcgtcg atgcgctcgt cgcccgggaa gtcggccgcc gtcaggtggc
     2461 gctccttcat gagcttcagc gctttgcgcg tgaaccggcc cgacggcgcg gccgccggta
     2521 cgtcggctcg gcgcggcggc tcctctttgc ggagctgcgg cggctcggcc gtgtccgcga
     2581 tccaggccac gggcgcgccg acggcgactt cgctgtgcgg ctcgtgctcg aaaaacaggt
     2641 agccgtcctg cggcgcgtcg acgtcaaacg tggtcttcgt cgtctcaagc gtcacgacgg
     2701 actgaccggc gtgcacgtgc gttcccttgt cgacgtgcca gcggacgacg atcgcgtgct
     2761 cgtcgttcgg attgatctgc ggcactctga cggcgacggg ctcactcatc gtagagctcc
     2821 aagcaggccg aacgcagcgc cgcgacgctc ggcagcacct cgagctcgag cttcagagcg
     2881 ctcgggatcg gccttggccg tgcggcgacg cgccgcgcgt gcagcccgcc accgcgacac
     2941 gcttccgcgg cggccgcgat gacctcgctc gccaagtcga aatgcgcggc accctcctcg
     3001 atgacgacga gcttccgcgt ccgggccacc gactcgacca cggggcgggc atcgaacggc
     3061 gacacgctga gcggcagcag tatctccacc tcgatctcct cggcgcgcag ctctgcagcc
     3121 acttgctccg cgacgaggct catgcggccg aacgccacga tcgtcaggtc cggagcgccc
     3181 gccggacgaa gcaccgtggt cggccacggg cccgtcgtct cgaacgcttc gtagcccggc
     3241 ggcaacgcgg ccgccgcgct ctcgcgatac agcagcttgt tctcgatgat cagcgtcggt
     3301 tccacgctgt cgcgcagtgc ggcgtaaagc gccgcgatcg acgcgcggcc gtgcagcacc
     3361 gcgacacgca gccccggcat gcccgcgaag tgcttctcga tgttctgcga atgcgtcggg
     3421 ccgtagccgc ggccgccgcc catcggcgtg cggacgacga ttgcgctcga cactcggccg
     3481 ttgtacatgc cgcgaaactt cgccgcatgg ttcagcaact gatcgaagca cagtcccaag
     3541 aaatcgccga acatgatctc gacgacgggg cgccggccgg cgagcgcaag cccattggcg
     3601 atgccaacga tcccggcttc gctgatcggt gtgttgatga cgcgctcggg gtgagcgtcc
     3661 gacaggcctc gagtcgcctt gaacgcgccg ccgtacggcg agcgaatgtc ctcgccgagc
     3721 agcaacacgc gggcgtcgtc ctcgagccac tcttgcaaag cgcgattgat cgcgacgagt
     3781 tgcgtaccgg gctcgagcgc cgcggctcgc cactcgatgc ttaagcgcac gtcggcggat
     3841 tcgtgtgcac taagcactgg cgctgccgcg gctcgctcga tcgcgcgcgc gacgcgctcg
     3901 cgaatctccg ctaggcccac gtccgcgtcg acttgctgct gcgccagaaa aacgttcagc
     3961 gggtcgcggc gcgcaaacgc cgcgacttct ttgggatcgc gatcgtcgtc gcctttcgaa
     4021 tgcgcggcga gccggtaggt gtcgacgcgc acgaacaacg ggccgccatc gtgcctgacg
     4081 gccgccgccg cttcgcgcaa ctgcgcggcc agcacgtagg ggcgccatgt ttcgccgtgc
     4141 agcgtgcgca tgccgaatgc cgccgctcgc gcgcagatgt cgccggccag cgtctcggcc
     4201 tgtgacgtcg actgcgcgta acgattgttc tcgagcacga cgagcagcgg cagccgccat
     4261 ttcgcggcca tgttgaacgt ctcgtagacg acgccttcac cgagcgttcc gtcgccgatg
     4321 cagaccgcga cgatgccgcc gtcgcctgcc agcttcttcg cgaacgcgag gcccgcgcct
     4381 acagggacga tgccgccctg aatgccgttg ctcatgaaac cttcggcgct caagtgctga
     4441 ctaccgccca agcccgcgca aacgccggtc gacttgccca tgacttcggc gaccagaccg
     4501 tcgggatcgt cggtccaggc caggtaatgc ccgtgacagc gatggttcga gacgatgacg
     4561 tccttacgcc gatcgagcgc cgccgccaac gcgacggccg acatctcctg gccgatgcac
     4621 gtgtgcgtcg tgcccgagag cttgccggca gcgaacagct cgagcagctg ctcttcgacg
     4681 gtgcgcacga ggtacgcctt gtcgaagatg tgccgctcga cgttcattgg agcttgctac
     4741 cgacgcgtgc gacgacatcc ggcatgctga ccatgtccgt ggcgtccgcg ccgtcgaaac
     4801 ggatcccgaa ctcctgctcc aggcccagca agaactccat gtgcttcatg gaattccagc
     4861 gcggctcgtc gagccgcgat agctgcggcg cgaagggcat tcgcttgccg aagacgcggc
     4921 cgaagaccgt ctcgacgcgc gcagcaatgt ccgtcgtgct cattttccct cctcgtgctc
     4981 gacctcggtg agaagcgcag cggcgatcaa tgcatgacct tgggcattcg gatgattatc
     5041 gatgcgcaac gtgtagaggc tcgcgtagtc gggctgcgcg gcgaacaggg ccacgccgtc
     5101 gacgacgccg gcggtcgagg cagccacgat gttgcgcacg gcctcgtagc gcgcattgtt
     5161 caatttaaga cccgtcgaga tctcatagct cggcaggatg acgacgacga gccgccctcc
     5221 ccacgcctgc accgagctcg aggcctgctc gagtgcgcgc tggaaatgcg acaagtccgg
     5281 cgccggcggc gcgcgccgag cggccgggcc gaagtcgagc agactgcgca actccttcat
     5341 cttgaagagc cgctcgagcg ggaaccgact cgcgacatcg agctcttcgc gtagctcttc
     5401 ggcgccgctt cgattgagcg gcaccaacac ttcgcggatg aacgcgtcga cttcgtgggc
     5461 ccgctcacgt agatgctgtg aataggcggg atcgtccaag tagctcgtca gcaacggttg
     5521 gtcagcctct tgcctgggct ccgcgaagtt ggtgttcacg aaccacagca cgagcggcgg
     5581 gcgcagcggc tgcacgtact cgcgcatgac gccgagctgc gacagcacac gcgatcccgc
     5641 gacgccgaaa ttcgccgtgc gcggatgccg ggcgcgcagc aattcgacgg cgctgcgcga
     5701 cggcgcgacg cagtggccga gggcgaacga ttcgccaacg actgcgatgt cgatcggacc
     5761 gtgcgcgaga cccggcggat tgttgaaccc gaggtcgtcg gatcgaaatt gaaagtagcc
     5821 gttgccctcg ttgcactcca cgacgtaggc attgctggcg ttcgaaagcg gcagaaggcc
     5881 gcgctcgagg accgcgctcg cgacgacgcc ggtcgtcccc gcggtctgcg cgacgccgga
     5941 gacggcatcg agcccacgtg cgcgaagctc gtcgatgacg tcgggccgca gccgctcgtc
     6001 gaaatcgact ccttgccgca gcgccgcgcg ttcccgcgcg atcacgcgtt gcgcgagcga
     6061 gtcatcggac cacagctccg gcgagcggcc gagcaagatc gcctcgcccg ccatcagagc
     6121 gaaagtaaga aaaacgatct cgagcgcgac acgctcgacg gctcgcgcgc ttccctgcag
     6181 cgcaactgcg aacgcgagca cgagcagcgc tgctgcagcc gccacggtcg ccgccacgaa
     6241 gcctggcgtc caaatcgcga gccatgccgg cacgcgataa acggcggccg cgagcgcgcc
     6301 tgcgccggcc accatgacga gcagccgcca cagtcggacg gtcgtcggtt cgtctacctt
     6361 cgccgaaaaa gaaagcatgt tgcacgtcgt cgatcgatca aggaaaaagg ag
//
LOCUS       DATLEE010000578         2404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7791807, whole genome shotgun sequence.
ACCESSION   DATLEE010000578 DATLEE010000000
VERSION     DATLEE010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7791807"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VM692_12555"
     CDS             complement(<1..309)
                     /locus_tag="VM692_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53047.1"
                     /translation="MLRQHEKIIAAVLAFGALAVSAVDAQEAPTYSATDSRASTSPVL
                     WQPSMNVFRRFDVPAERMYEFYGGVLGFKQLSTLNVGNGGGVARFQAGAQELKLTRRV
                     G"
     gene            380..1741
                     /locus_tag="VM692_12560"
     CDS             380..1741
                     /locus_tag="VM692_12560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUQ53048.1"
                     /translation="MWKILVVLLVLGVGAAALWVNAGQAEGPAIEIAGPGVIGQTGEV
                     AVNVTAPGGTLTGLTVALVQGETKTPLFDLSPETAAALTTNGDEVSLTRPAGKKELPE
                     LKAGEAQVTVTAVRPVLFGLRQATATATRTLEVRLTPPQLAVLSQFHYINHGGSEMVV
                     YRVTPPDTESGVRVGDREYRGFPAGGSDPALRVAFFALLWDQGLNTPITVFARDSVGN
                     EGSGSFDFRVFPKQFRSSTISLDDRFLARVVPPILQNSTELKVDDPSDYLAGYLAINR
                     ELRRMNNETITKLALETAPEILWQGPFKQLINTAVEAGFADQRTYVYNGKDVDHQVHL
                     GFDLASTAAAPVRAANRGRVVHAGWLGIYGNCVILDHGMGLQSLYAHLSSISVARGDL
                     VDSEAELGRSGSTGLAGGDHLHFTMLLGGNAITPIDWWSAQWVQDRVLRKFTDAGLTP
                     PGS"
     gene            complement(1751..>2404)
                     /locus_tag="VM692_12565"
     CDS             complement(1751..>2404)
                     /locus_tag="VM692_12565"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HUQ53049.1"
                     /translation="AIMTPRTEVVWLDINAAPADIVAQLDARRLSRFPVCRETIDHPV
                     GVVHMKDLARVALAGQSVVLADILLQPLVVLDGTPVLKVLEGFRREGMHMAIVVDEYG
                     ATQGVVTLADIIEAVAGTLPETGNDLVSSLVKRSDGSWLVDGSVGIDEFEDRLGVSGL
                     RGSRTFDTVAGYALYRLGRLPVVGDTFTDRTGAYEIVDMDGRRIDKLIYRPRVDEEG"
BASE COUNT          420 a          799 c          793 g          392 t
ORIGIN      
        1 gccgacgcgg cgagtgagct tgagctcctg cgctccggcc tggaagcgtg cgacgccgcc
       61 gccgttgccg acgttgagcg tcgagagttg cttgaagcca agcacgccgc cgtagaactc
      121 gtacatgcgc tccgccggaa cgtcgaagcg gcggaacaca ttcatcgacg gttgccagag
      181 caccggcgac gtcgacgcgc gcgagtccgt cgcggagtac gtcggcgctt cttgcgcatc
      241 gacggcgctc accgcgagcg caccgaacgc gagcactgcc gcaatgatct tctcgtgctg
      301 tcgcagcatt gcgacctcct tcgcactacg cgccggaagc gagtgtgaat cacgcggcct
      361 gtcggtacaa tcgcgggcca tgtggaaaat actcgtcgtc ctgctggtcc tcggagtcgg
      421 cgctgcggcg ctgtgggtaa acgccggaca ggccgagggg cctgccatcg aaatcgcggg
      481 tcctggcgtc atcggccaga ccggtgaggt cgccgtcaac gtcactgcgc cgggcggcac
      541 gctgacgggt ctgaccgtgg cgctggtgca aggcgagacg aagacaccgc tgttcgactt
      601 gtcgccggag accgctgcgg cgctcacgac gaacggcgac gaggtcagcc tgacgcgacc
      661 ggccggcaaa aaagaattgc ccgagctcaa ggccggggaa gcgcaagtca cggtgactgc
      721 cgtgcggccg gtgctgttcg gactgcggca agctacggcc acggcaaccc gcacgctcga
      781 ggtgcggctg acgccgccgc agctggcggt gctatcgcag ttccactaca tcaaccacgg
      841 cggctcggag atggtcgtct atcgcgtgac cccgcccgat accgagtcgg gcgtgcgcgt
      901 cggcgaccgc gagtatcgcg gcttcccggc cggcggctcc gatcccgcgc tgcgcgtcgc
      961 gttctttgcg ctgctgtggg accaaggcct caacacgccg atcacggtgt ttgcgcgcga
     1021 ctccgtcggt aacgaaggca gcggcagctt cgacttccgc gtgttcccga aacagttccg
     1081 ctccagcacg atcagtctcg acgaccggtt tctcgcgcgt gtcgtgccgc cgatcttgca
     1141 gaactcgacg gagctgaagg tcgacgaccc gagcgactac cttgcgggct acctcgcgat
     1201 caaccgcgag ttgcgccgca tgaacaacga gacgatcacg aagctcgcgc tagagacggc
     1261 gccggagatc ctctggcaag gcccgttcaa gcaattgatc aacacggccg tcgaagccgg
     1321 gttcgccgac cagcggacct atgtctacaa cggcaaggac gtcgatcatc aagtgcatct
     1381 cggcttcgat ctggcgtcga cggctgcggc gcctgttcgc gcggcgaatc gtggccgagt
     1441 cgtgcacgca ggctggctcg gtatctacgg caattgcgtg atcctcgatc acgggatggg
     1501 cctgcaatcg ttgtacgcgc acttgtcctc gatcagcgtg gcgcgcggcg acctcgtcga
     1561 cagcgaggcc gagctcggtc gcagcggctc gacgggcctc gcgggcggcg accacttgca
     1621 tttcacgatg ctgctcggcg gcaacgccat cacgccgatc gattggtgga gcgcgcagtg
     1681 ggttcaggat cgagtgttgc gaaagttcac cgacgcggga ctgacgccgc cgggctcctg
     1741 acgccgaagg tcacccttcc tcgtcgacgc gcggccggta gatgagcttg tcgatgcggc
     1801 ggccgtccat atcgacgatt tcataggcgc ccgtgcggtc cgtgaacgtg tccccgacga
     1861 ccggcaatcg cccgagccga tacagcgcat aaccggccac ggtgtcgaac gtccgcgacc
     1921 cgcgcaggcc cgacactccg agtcgatcct cgaactcgtc gatgccgaca ctaccgtcga
     1981 cgagccacga gccgtcgcta cgcttgacga gcgacgacac gaggtcgttg ccggtctcgg
     2041 gcaacgtacc ggcgacggct tcaatgatgt ccgcgagcgt cacgacgccc tgcgtcgcgc
     2101 cgtactcgtc gacgacgatc gccatgtgca taccttcgcg cctgaagccc tcgagcacct
     2161 tgagcaccgg tgtgccgtcg agcaccacga gcggctgcaa caggatgtcg gcgagcacga
     2221 cgctttggcc ggccaacgcg acgcgcgcga ggtccttcat gtggacgacg cccacagggt
     2281 ggtcgatcgt ctcgcgacaa acaggaaagc gtgacaagcg acgcgcatcg agctgcgcaa
     2341 cgatgtccgc aggcgccgcg ttgatgtcga gccagacgac ctcggtgcgc ggcgtcatga
     2401 tggc
//
LOCUS       DATLEE010000579         2583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6306031, whole genome shotgun sequence.
ACCESSION   DATLEE010000579 DATLEE010000000
VERSION     DATLEE010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6306031"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="VM692_12570"
     CDS             complement(<1..1542)
                     /locus_tag="VM692_12570"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ53050.1"
                     /translation="MPSSPRLAQWFGVATVAALGAIPVLSVVETERGGHSLTVAVVGE
                     YCNDCHDAISQSGGVVLEAAAFDDVGAHAELLERVVRKLKARTMPPPENPRPTAAAYE
                     RVTTYLETALDASAAAAPNPGTLPQLHRLTRTEYRNTIRDLLAIENLPAELDYELLLP
                     ADNSSSGFDNISDLLFVSPAIMERYLEAARKLSRLAVGDPELPVMVNIHRMPLHLPQD
                     DAGAGLPLGTRGGLATDSYFPLDGEYSFKVQLERAPREQHELEITVDGARVASATIGL
                     TNLANPPQRVFEFRVPIDAGPHRVGATFVERTGALDERTLRPQRRSRGTLPAMEIVTI
                     SGPYAPSGPGDTPSRERLFVCRPQTVAEQTACAHEILSTLARRAYRRPVEAADVDELW
                     PFYEAGVAERGFERGIQRALERVLVSPQFLYRIERIPPGAAPGTTFAVNDLELASRLS
                     YFLWASMPDDALLDVAAAGELTRPQVLSREVARMLADPRADALVENFAAQWLFLRDVA
                     GRNPDP"
     gene            1607..>2583
                     /locus_tag="VM692_12575"
     CDS             1607..>2583
                     /locus_tag="VM692_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ53051.1"
                     /translation="MLRVKIYPEIRLVALTGCALAGAVASGAEPALIAAVRSQNEARV
                     AAVLSENPDVNAVAADGSTALLAAVELDDVALAERLLAAGARVDIANRNNISPLAVAA
                     MNGNAAIVERLLARGANVDELSGEGQTALMLAAQNGRPEAVALLLEKGANVQAIEPFR
                     GQTALMWAAGAGNADATALLLAHGARVEDKSKAGFTALLFAVRNAQLDALRVLLERGA
                     NANAVAPDGTSALGMAVVNAYYEVASVLLEHGADPNLPDPRGSALHTIAWLRRPGASG
                     TAAVGGEAEAVPVPVGKVTSLELAKQLLEHGANPNVRIDWKEQRFGKEGG"
BASE COUNT          393 a          867 c          943 g          380 t
ORIGIN      
        1 cggatcgggg ttgcggccgg ccacgtcgcg caggaacagc cattgcgctg cgaagttctc
       61 gacgagcgcg tcggcacgcg ggtctgcgag catgcgcgcg acctcgcgcg aaaggacttg
      121 cggccgcgtc agctcgccgg ccgcggcgac gtcgagcaac gcgtcgtcgg gcatgctggc
      181 ccaaaggaaa tacgacaggc gcgacgcgag ctcgagatcg ttgacggcga acgtcgtgcc
      241 gggcgcggcg ccgggaggga ttcgctcgat tcgatacagg aactgcgggc tcacgagcac
      301 gcgctcgagc gcgcgctgga tgccgcgctc gaaaccgcgc tcggcgacgc cggcttcgta
      361 gaacggccag agctcgtcga cgtcggcggc ctcgacgggc cgccggtaag cgcggcgggc
      421 gagagtcgac aggatctcgt gcgcgcacgc cgtttgctcg gcaaccgtct gcggccggca
      481 gacgaatagg cgctcgcggc tcggcgtgtc gccgggcccg ctcggcgcgt agggcccgct
      541 gatcgtcacg atctccatgg cgggcagcgt gccgcggctg cggcgctgcg ggcgcaacgt
      601 gcgctcgtcg agcgcgcccg tgcgctcgac gaacgtcgcg ccgacgcgat gcgggccggc
      661 gtcgatcggc acgcggaact cgaacacgcg ctgcggcggg ttcgcgaggt tcgtcaggcc
      721 gatcgtcgcg gacgcgacgc gcgcgccgtc gacggtgatc tcgagctcgt gctgctcgcg
      781 cggcgcacgc tcgagctgca ccttgaacga atattcgccg tcgagcggga agtagctgtc
      841 ggtcgcgaga ccgccgcgcg tgccgagcgg caggcctgcc cccgcatcgt cctgcggcag
      901 atgcagcggc atccggtgga tgttgaccat gacgggcagc tcgggatcgc cgacggcgag
      961 gcggctgagc ttgcgtgccg cctcgaggta gcgctccatg attgccggtg acacgaacag
     1021 caggtcgctg atgttgtcga agccgctgct cgagttgtcc gccggcaaga gcagctcgta
     1081 gtcgagctcg gccggcaggt tctcgatcgc aagcaagtcg cggatcgtgt tgcggtattc
     1141 ggtgcgcgtg aggcggtgca gttgcggcaa cgtgccgggg ttcggcgcgg ccgcggcgct
     1201 cgcgtcgagc gccgtctcga gatacgtcgt cacgcgctcg taagccgcgg cggtcggccg
     1261 cgggttctcg ggcggcggca tcgtccgcgc tttgagcttg cgtacgacgc gctcgaggag
     1321 ctcggcatgc gcgccgacgt cgtcgaaggc ggcggcctcg agcacgacgc cgcccgattg
     1381 cgagatcgca tcgtgacaat cgttgcagta ctcgccgacg acggcgaccg tcagcgagtg
     1441 cccgccgcgc tcggtctcga cgacgctcaa gaccgggatt gcacccaagg cggcaaccgt
     1501 cgcgacgccg aaccactgcg ctaaccgcgg cgaagaaggc aagtcagctc ccccgaacag
     1561 gatagtcatt gtatattgcg gtcgaactcg agtcgcagga acacccatgc tccgagtcaa
     1621 gatttaccca gagattcgcc tcgtcgctct aacgggatgc gcgctggccg gcgccgtcgc
     1681 gtccggggcc gagcccgcat tgatcgcagc cgtgcgcagc cagaacgaag cgcgcgtcgc
     1741 ggccgtgctc agcgaaaacc ccgacgtcaa cgccgtcgct gccgacggct cgacggcgct
     1801 gctcgcggcc gtcgagctcg acgacgtcgc gctcgccgag cggctgctcg ctgccggcgc
     1861 gcgcgtcgac atcgcgaatc gcaacaacat ctcgccgttg gccgtcgcgg ccatgaacgg
     1921 caatgcagcg atcgtcgagc gcttgctcgc gcgaggcgcc aacgtcgacg agctttcggg
     1981 cgagggtcag acggcgctga tgcttgcagc gcagaacggc cgtcccgagg ccgtcgcctt
     2041 gttgctcgag aagggcgcga acgtgcaagc catcgagccg ttccgcggtc agacggcatt
     2101 gatgtgggcc gccggcgccg gcaacgccga cgcgacagcg ctgctgctcg cgcacggcgc
     2161 gcgcgtcgag gacaagtcga aggccggctt cacggcgttg ctgttcgccg tgcgcaatgc
     2221 gcagctcgac gcgttgcgcg tgctgctcga gcgcggcgcc aatgcgaacg ccgtggcgcc
     2281 cgacggcacg agcgctctcg gcatggccgt cgtcaacgct tactacgagg ttgcgtcggt
     2341 gctgctcgag cacggcgccg atccgaactt gcccgacccg cgcggctcgg cgctgcatac
     2401 gatcgcgtgg ttgcgcaggc ccggtgcgtc cggcaccgcg gccgtcggcg gcgaggccga
     2461 ggccgtgccg gtgcccgtcg gcaaggtcac gagcctcgag cttgcgaaac aattgctcga
     2521 gcacggcgcg aatccgaatg tgcgcatcga ttggaaggag cagcgcttcg gcaaggaggg
     2581 cgg
//
LOCUS       DATLEE010000580         4823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3344039, whole genome shotgun sequence.
ACCESSION   DATLEE010000580 DATLEE010000000
VERSION     DATLEE010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3344039"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VM692_12580"
     CDS             <1..994
                     /locus_tag="VM692_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ53052.1"
                     /translation="LPALALLAAASLAAQAQQPAPPGEVIGVGNFAHIVADLDKTLGF
                     YRDAIGLEITVQQGFAPNDAIAKLGATEGGQSRIAVLDVPGLELGVELIEYKDIQRNV
                     QRPRFYDPGAANLALRMRDIDAVYERVAKFPGAKVITAGGKPVTIKTPNGELHAVFVQ
                     DPDGFVVELAEIGGAIAADAPAGNIVGGAFEPTVANSEASIKFYNELLGFNFPAPAAW
                     NDNQQMAGTAGAPGASFRQSRTNIPGTSVAFTFIEWRNIDGKMLTGRTQDPGTTVLQL
                     IVRDVAALTAKLKAAGVPIVTTGGAPVEVVPGLNISIVRDPSNVLLELVQRSPR"
     gene            1094..2383
                     /locus_tag="VM692_12585"
     CDS             1094..2383
                     /locus_tag="VM692_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53053.1"
                     /translation="MLPKGHWLLHTFAAAALCAGHAAACAQSAALPDETANEQRSSLP
                     APELTQPPAIEPPAVEQPAAEPSAVEPPAVESPVVEPPPAEPLASEHAGGEAPPVGIG
                     NGDVLAMVEAQFSDATIVSIIGANDARFDLSPRALVGLKGAGVSEDVIAAMIAAAAEK
                     RDVAEPELAATAETQASLDYAKLTQMIEQLAAKQEAAAAERRDPTPPASRADPAPHVW
                     LVGPAEKAAIAPTIAQVAFAGGGRRNNERMKNLQGIAGQALAFVNPAVSGIATTLGSL
                     FRPEEQRTAVWALAATSSTRVLDFDPVFEMDFAHIPGVDPDRYQPTIVQLVATNDNYR
                     LVAAAETDGSNTGALPSGPIIEESVATEATRLGRGQYRVTPRAALPPGEYALVLRPIA
                     ESKRARRREAEASLGELLGGSTSQILYLTWEFSIAAR"
     gene            complement(2388..3128)
                     /locus_tag="VM692_12590"
     CDS             complement(2388..3128)
                     /locus_tag="VM692_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HUQ53054.1"
                     /translation="MTYCIGIRLDAGIVFLSDSRTNAGVDQVRTFRKTHRFERPGERI
                     IVVQTAGNLALSQSVVSELRTAFRADAPGVTLWNAKTMFDAASVVGEAVRSVHARDAV
                     TMQSFGVEFNLSLIVGGQIRGEQPRLFNVYNVGNFIESTDETPYFQLGEAKYGKPILD
                     RVITADLSLHEAAKCALVSMDSTMKSNLSVGLPLDLVCYTTDSFRIDQHKLIDDQDAY
                     FSSIRRSWGQQLRKVFDELPNPQWWSNG"
     gene            complement(3261..3644)
                     /locus_tag="VM692_12595"
     CDS             complement(3261..3644)
                     /locus_tag="VM692_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ53055.1"
                     /translation="MAIFTHVTVGTNDLARARNFYDKVLGALGYKRLTDLGDNGSIWG
                     ESTPEFFVLKPANGLPANWGNGVTVSFVAPSRAAVAKFHKEAIALGAKDEGAVGPRSW
                     HPTAYAGYLRDLDGNKICAYCFSSE"
     gene            3790..4650
                     /locus_tag="VM692_12600"
     CDS             3790..4650
                     /locus_tag="VM692_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015886898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HUQ53056.1"
                     /translation="MREINYKPPSSSSVFRLGASLLVGLVAIYVLFASWYIVDQGERA
                     VVLRLGRTVAEAGPGPHLKLPWVDNVRKITVQNQNKRYQNLEAYSRDQQPANLTVSVT
                     FVVSNASEIYSQYGDLEVAVTRLIDPRLTAGVKTIFGQYDAVRAIQERAALNSDFASA
                     VTSAISGPINIISVQIENIDFSEAYEQSVEQRMLAQVEIQRREQNLRTTEVEAQIART
                     RAEGEAEAIRLRGEAEASAIRARADALRANGELVQLQAVEKWDGKLPATMVPNSAMPF
                     INLQPTSRND"
BASE COUNT          859 a         1587 c         1616 g          761 t
ORIGIN      
        1 tttgccggct ttggcgctgc tcgcggctgc gtcgctcgcg gcgcaggctc agcagccggc
       61 ccctccgggg gaggtcatcg gcgtcggcaa tttcgcgcac atcgtcgcgg acctcgacaa
      121 gacgctcggg ttctaccgcg acgcgatcgg tcttgaaatc accgtgcagc agggcttcgc
      181 gccgaacgat gcgattgcga agctcggggc gacggaaggg ggccaatcgc ggatcgccgt
      241 gcttgacgtc ccggggctcg agctcggcgt cgagctcatc gagtacaagg acattcagcg
      301 caacgtgcag cgcccgcggt tctacgatcc cggtgcggcg aatctcgcgc tgcgcatgcg
      361 cgacatcgac gcggtctacg agcgcgttgc gaagtttcca ggcgccaagg tcatcacggc
      421 cggcggcaag cccgtgacga tcaagacgcc gaacggcgag ctgcacgccg tgttcgtgca
      481 agaccccgac ggtttcgtcg tggagctggc cgagatcggc ggcgcgatcg cggccgatgc
      541 gccggcgggc aacatcgtag gcggcgcgtt cgagccgacg gttgcgaaca gcgaagcgag
      601 catcaagttc tacaacgagc tgctcggttt caattttccc gcgcccgctg cgtggaacga
      661 caatcagcaa atggccggta ccgcaggcgc gccgggcgca agctttcgac agagccgcac
      721 gaacattccc ggcacctcgg tggcgttcac gttcatcgag tggcggaaca tcgacggcaa
      781 gatgctgacg ggccgcacgc aggacccggg cacgaccgtg ttgcagctca tcgtgcgcga
      841 cgtcgcggcg ctgacggcga agctcaaagc cgccggcgtg ccgatcgtga cgacgggcgg
      901 agcgcccgtc gaggtcgtgc cgggcctcaa catctcgatc gttcgcgacc cgagcaacgt
      961 gctgctcgag ctcgtgcagc gctcgccgcg ttgaagcgcg tcgccgatcg gcgacgcctg
     1021 aatggtcaaa aggttgcggc cggcgcgcgc aggccgatag cattggcgct tcacgccgct
     1081 cagggataga gacgtgctgc caaagggaca ctggcttctg catacatttg ccgctgccgc
     1141 gctgtgcgcg ggacatgccg ccgcgtgcgc gcagtctgcg gcgttgcccg acgagaccgc
     1201 gaacgagcag cgaagctcct tgcctgcgcc ggagctcacg cagccgccgg cgatcgagcc
     1261 gccggccgtc gagcagccgg ccgccgagcc gtcggccgtc gagccgccgg cggtcgagtc
     1321 gccggtcgtc gagccgccgc ccgcagagcc gcttgcctcc gagcacgccg gtggcgaagc
     1381 gccgcccgtc ggcatcggca atggcgacgt cctcgcgatg gtcgaggcgc agttcagcga
     1441 cgcgacgatc gtctcgatca tcggtgcgaa cgacgcgcgg ttcgatctat cgccgcgcgc
     1501 gctggtcggc ctcaagggcg caggagtttc ggaggacgtc atcgcagcca tgatcgctgc
     1561 cgcggccgag aagagagacg tcgcggagcc cgagctcgcc gccacggccg agacgcaggc
     1621 ttcgctcgac tacgcgaagc tcacgcagat gatcgagcaa ctcgccgcca agcaggaagc
     1681 ggcagcggcc gagcgtcgcg accccacgcc tccggcctcg cgcgccgatc ccgctccgca
     1741 cgtatggctc gtcggtccgg cggagaaagc ggcgatcgct ccgacgatcg cccaagtggc
     1801 ctttgccggc ggcggccgac gcaacaacga gcgcatgaag aacctgcaag gcatcgcagg
     1861 gcaggcgctt gcgttcgtga acccggccgt cagcggtatc gcgacgacgc tcggcagctt
     1921 gttccggccc gaggagcagc gcacggccgt ctgggcgctg gccgcgacgt cgagcacgcg
     1981 cgtgctcgac ttcgatccgg tgttcgagat ggatttcgcg cacattcccg gcgtcgatcc
     2041 ggaccgctat cagcccacga tcgtccagct cgtcgcgacg aacgacaact atcgtctcgt
     2101 ggccgctgcc gaaaccgacg gctcgaatac cggcgctctg ccgagcgggc cgatcatcga
     2161 ggaaagcgtc gcaacggaag ctacgcggct cggccggggg caatatcgcg ttacgccgcg
     2221 agcggccctg ccgcccggcg agtacgcgct cgtgctgcgc ccaatcgcgg agagcaaacg
     2281 cgcgcgccgc cgggaagccg aagcgtcgct cggcgagctg ctcggcggca gcacgagtca
     2341 gatcctgtac ctgacgtggg aattctcgat cgcggcgcgc tgatgcgtca gccgttgctc
     2401 caccactggg ggttcggcag ctcgtcaaaa accttgcgca actgttggcc ccacgagcgg
     2461 cggatcgagc tgaaatacgc gtcctgatca tcgatgagct tgtgctggtc gatcctgaag
     2521 ctgtccgtcg tgtagcagac gaggtcgagc ggcaagccga cggataggtt cgacttcatc
     2581 gtcgagtcca tcgagacgag cgcgcatttc gccgcttcgt gcagcgacaa atccgccgtg
     2641 atgacgcggt cgagaatcgg cttgccgtac ttggcctcgc cgagctggaa gtagggcgtc
     2701 tcgtcggtcg actcgatgaa attgccgacg ttgtagacgt tgaacagacg cggctgctcg
     2761 ccgcgaattt gcccgccgac gatcaagctc aggttgaact cgacgccgaa gctctgcatg
     2821 gtgaccgcat cgcgcgcgtg cacactgcgc acggcctcgc cgacgacgct cgcggcgtcg
     2881 aacatcgttt tcgcgttcca caacgtgacg cccggtgcat cggcccgaaa tgccgtgcgc
     2941 agctcggaca ccaccgactg agacaacgcg agattgccgg ccgtctgcac gacgatgatg
     3001 cgctcgccgg gacgctcgaa gcggtgcgtc ttgcgaaacg tgcgcacttg atcgacgccg
     3061 gcgttggtcc gcgagtcgga aaggaacacg atgccggcgt cgagccgaat gcctatgcaa
     3121 tacgtcatgt cgtcatggta ccgccgccgc cggcgccgag tcgcgccgcc gagcgcggct
     3181 cgatcgtcga cgaccacgaa gggggacgac cctttgaagt cgccgaaggc gaccgagccg
     3241 agctgcggcg tgcagcgcga ttactcgctc gagaagcaat aggcgcagat tttgttgccg
     3301 tcgagatccc gcaagtagcc cgcgtaagcc gtcggatgcc agctgcgtgg cccgaccgcg
     3361 ccttcgtcct tggcgccgag cgcgatggct tccttgtgga acttcgcgac ggcggcgcgg
     3421 ctcggcgcaa cgaagctcac ggttacgccg ttgccccagt tcgccggcag gccgttcgcg
     3481 ggcttcagca cgaaaaactc cggagtcgac tcgccccaga tcgagccgtt gtcgccgagg
     3541 tccgtgaggc gcttgtagcc cagcgctccg aggaccttgt cgtagaagtt gcgggcgcgt
     3601 gccaagtcgt tcgtgccgac ggtcacgtgc gtgaagattg ccatgctctt ttccttttct
     3661 gtggccaggt gggaacgaga aaacgctgca tagcataagg aggagcgctg cagaaagctg
     3721 ctatgatttc gcgcgcttcg gcctccgacg aaatctccag cagccgcgtt gccaacaagg
     3781 gggaatgccg tgagggaaat caactacaag ccaccgagct cgagcagcgt gtttcgtttg
     3841 ggcgcgtcgt tactcgtcgg gctcgtcgcc atctatgtgt tgttcgcgtc gtggtacatc
     3901 gtcgatcaag gcgagcgcgc cgtcgtgctg cggctcggcc gcacggtcgc cgaggcgggc
     3961 ccgggcccgc atctgaagct gccgtgggtc gacaacgttc gcaagatcac cgtgcagaat
     4021 cagaacaagc ggtatcaaaa cctcgaggcg tattcgcgcg accagcagcc agcgaatctc
     4081 acggtcagcg tcacgttcgt cgtgtcgaac gcgagcgaaa tctactcgca gtacggcgac
     4141 ctcgaagtcg ccgtcacgcg gctcatcgat ccgcgcctga cggccggagt caagaccatt
     4201 ttcggccagt acgacgccgt gcgcgcgatt caggaacgcg ccgcgttgaa cagcgacttc
     4261 gcgtccgctg tcactagcgc gatcagtggc ccgatcaaca tcatcagcgt gcagatcgag
     4321 aacatcgatt tctccgaggc ctacgagcag agcgtcgagc aacgcatgct cgcgcaggtc
     4381 gagattcagc gccgcgagca aaacttgcgc acgaccgagg tcgaagcgca aatcgcgcgc
     4441 acgcgcgccg aaggcgaagc cgaagcgatc cggttgcgcg gcgaagccga agcctcggcg
     4501 atccgcgctc gcgctgacgc actgcgcgcc aacggcgagc tcgtgcagct gcaggccgtc
     4561 gagaagtggg acggcaagct gccggcgacg atggtgccga actcggcgat gccgttcatc
     4621 aacctgcagc cgacgagccg gaacgactag cgaaagcgca cggctccgta ggagcgcggg
     4681 ctcatcgcgg cgctgccttc gtaggcagcg agcgccagcg cgccgggtcg tgcagtccca
     4741 gagcgaacag catccccgcc gtgacgaacg tgacgccgac gagctgcaac gccgtcggcg
     4801 tctcgtgcag caacggaatt gcg
//
LOCUS       DATLEE010000581         3845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9640303, whole genome shotgun sequence.
ACCESSION   DATLEE010000581 DATLEE010000000
VERSION     DATLEE010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9640303"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VM692_12605"
     CDS             complement(<1..576)
                     /locus_tag="VM692_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53057.1"
                     /translation="MHALVIALFSSVLVVEYLVEQRHLLHPYFVLLPELFSGIAMLVV
                     LLKIMNGTRVHFDWRYGLFIFVLLFTIVFAYSVEDVPTGAMLAGVRSYIKFLPFFLLP
                     AVHRFTPRQLQTQLVFLLVIAVLETPLAFYQRFVEFAASMHTGDPVRGTFATSSGLSL
                     FMVCVIAGIVIAHLRGHVRLATMMLLSAWLFS"
     gene            complement(591..1892)
                     /locus_tag="VM692_12610"
     CDS             complement(591..1892)
                     /locus_tag="VM692_12610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ53058.1"
                     /translation="MHEQAALAPPRRADDKLDILFVTSDLYPPFRPAAKAVFAEGLAA
                     RGHRIDWLMQAAAPTDGSGPQRFKGGTAYVAPTNAGASRMARLRRHLADIRNDLRVFG
                     LLKRHRYSLVQIKDKYIGALFAIAAAKLHRVPVFYWLAYPHGEASLYAAESGVARYEV
                     LYRLRGMLQRWLLYRVIMPSCTHVFVQSEQMRRDVASEGIPLESMTAVPSSLNLRDLD
                     SAAPSTAPPAASPAKPTIVYLGTLLRERQLDFLVRAHALVVAEVPHAQLLFVGSGWMP
                     DDEQLLQREAQRLGIERNVTITGWLPMPDAWQHVRQSALCVSPYLPVPILRSTSPTKL
                     IEYMALGKPVVANDHPEQADVLQQSGAGLVCSWDEREFAKAIVELLKDPARCRKMGAA
                     GRSFVAEHRTHWAMVELVAGRYRKHLTEPPKQPKPRMFARV"
     gene            complement(1885..2481)
                     /locus_tag="VM692_12615"
     CDS             complement(1885..2481)
                     /locus_tag="VM692_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53059.1"
                     /translation="MPSFAASSWGHDDELDGALLLAGLREPAMEGRHCFQTTYVRDRY
                     REYFAHDGFRLVWIVREPRAAVRSLLGDGQRSLPRRTALGLAGKSSGGQLVSRLEKAC
                     AAYVESMRQTLELKERLGERMAVVDYDGLVADRVRLLPALCRFASVPCDSRVLRHLHG
                     KSVRKGGLASWEGSIVDDLAMPTYRRARLVATLGAAHA"
     gene            2875..>3845
                     /locus_tag="VM692_12620"
     CDS             2875..>3845
                     /locus_tag="VM692_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HUQ53060.1"
                     /translation="MRWWLNTLPALALIVAGSSEAATYYVRNGGSDSADGRSHSTAWA
                     TLSKVNAYSFAAGDVVLLHEGDRFVGQVTVDWAGNTTARSVLGAYYLDASSNAVRGYR
                     SARPTIDGEDRLPATRYDAMVTVRANRVRVENLRVQNSEARGIATIESQDTEIVGCAV
                     YNVYNAGVHFLKSLRSLAENNFVTGAGIGNQQDGSPWGASIESVASHDTVVRNNTISE
                     SYGEGINTHTGSQRTLIERNYLFGVRAVGIYIDGSPDATVRRNIVVGTTTSAYWRGGS
                     SVGSGIVLNNESYHYPVGGGSLSTSVQAKRAKIYGNLVSFTSTGIAF"
BASE COUNT          713 a         1214 c         1307 g          611 t
ORIGIN      
        1 gctgaacagc cacgccgaca gcagcatcat cgtcgcgagc cgcacgtggc cgcgaaggtg
       61 cgcaatcacg atgccggcaa tcacgcaaac catgaacaag ctgaggccgc tcgacgtcgc
      121 gaacgtgccg cgcacggggt cgccggtgtg catcgacgcg gcgaactcga cgaagcgctg
      181 gtaaaacgcg agcggtgtct cgagcactgc gatcacgagc aggaagacga gttgcgtttg
      241 cagctggcgc ggcgtgaacc gatggaccgc cggcaacagg aagaatggca ggaacttgat
      301 gtacgagcgc acgcccgcga gcatcgcgcc cgtcggcacg tcttcgacgg agtaggcgaa
      361 gacgatcgtg aacaacagca cgaagatgaa cagcccgtag cgccaatcga agtggacgcg
      421 cgtgccgttc atgatcttca gcagcacgac gagcatcgcg atgcccgaga acagctcggg
      481 caacagcacg aagtacgggt gcaagaggtg ccgctgctcg acgagatact cgacgacgag
      541 aaccgaggag aacagtgcta tgacgagcgc gtgcatgtcc ggcgcctgcg ttaaacgcgc
      601 gcgaacatcc tgggcttcgg ctgcttcggc ggctcggtca ggtgcttgcg atagcgccct
      661 gccacgagct cgaccatggc ccagtgcgtg cggtgctccg cgacgaagct gcgcccggcg
      721 gcgcccattt tccggcagcg cgcgggatcc ttcagcagct cgacgatggc tttggcgaac
      781 tcacgctcgt cccacgagca gacgagaccc gcgccactct gctgcaggac gtcggcttgc
      841 tcggggtggt cgttcgcgac gacgggcttg ccgagtgcca tgtactcgat gagcttcgtc
      901 ggcgacgtcg agcgcaggat cggcaccggt aggtaaggcg acacgcagag cgccgattgc
      961 cgcacgtgtt gccacgcatc gggcatgggc agccaacccg tgatcgtgac gttgcgctcg
     1021 atgccgagcc gctgcgcttc acgctgcaac agctgctcgt cgtcgggcat ccaaccgctg
     1081 ccgacgaaca gcagctgcgc gtggggcacc tcggcgacga ccagcgcgtg cgcgcgcacg
     1141 aggaagtcga gctgccgttc gcgcagcagg gttcccaaat acacgatcgt gggcttggcg
     1201 ggacttgccg ccggcggcgc cgtgctgggg gccgcggaat cgaggtcgcg cagattcagc
     1261 gagctcggca cggccgtcat ggactcgagc ggaatccctt cgctcgcgac gtcgcggcgc
     1321 atttgctcgc tctgcacgaa aacgtgcgtg caggacggca tgatgacgcg gtacaacagc
     1381 caacgctgca gcatgccgcg cagccggtac agcacctcgt agcgcgcgac gccgctctcg
     1441 gcggcgtaca gcgaagcctc gccgtgcggg taggcgagcc agtagaacac gggcacgcga
     1501 tgcagcttcg cagcggcgat cgcgaacagc gcgccgatgt acttgtcctt gatctgcacg
     1561 agcgaatagc gatggcgctt caggagcccg aacactcgca ggtcgttacg aatgtcggcc
     1621 aggtgacggc gtagccgggc catgcggctg gcgccggcat tcgtgggcgc cacgtaggcg
     1681 gttccccctt tgaagcgctg ggggccgctg ccgtcggtcg gcgccgcagc ctgcatgagc
     1741 cagtcgatgc ggtgcccacg cgccgccagg ccttcggcga acacggcttt ggccgcggga
     1801 cgaaacggcg gatagagatc cgaggttacg aacaaaatgt cgagcttgtc gtcggcgcgg
     1861 cgaggcgggg ccagcgcagc ttgctcatgc atgggcggcg cccagcgtgg cgacgagccg
     1921 cgcccgtcgg tacgtaggca tcgcgagatc gtcgacgatc gagccctccc agcttgcaag
     1981 cccgcctttg cggacgctct tgccgtgcag atggcgcaac acccggctat cgcacggcac
     2041 cgaagcgaat cggcatagtg ccggcaacag ccggacccgg tccgcaacga gcccgtcgta
     2101 gtcgacgacg gccatgcgct ccccgaggcg ctccttgagc tccaacgtct gccgcatgct
     2161 ctcgacatag gccgcgcaag ccttctcgag ccgggaaacc agctggccgc cgctcgattt
     2221 gcccgcgagc ccgagcgcgg tccggcgggg cagcgacctt tgcccgtcgc cgagcagtga
     2281 gcgcacggcg gcgcgcggct cgcggacgat ccagacgagc ctgaagccgt cgtgagcgaa
     2341 gtactcgcgg tagcggtcgc ggacgtaggt cgtttgaaaa cagtgccggc cttccatcgc
     2401 cggctcccgg aggccggcga gcagtagggc gccgtcgagc tcgtcgtcgt ggccccatga
     2461 cgaagccgcg aacgaaggca accgcttgaa gaggctcgta atagcagtcg ccgccgagcc
     2521 gtcgcagccg gcgatcagca gcgcgtccgg aaaagcgtcg aggcggctca gtaggcgacg
     2581 ctcgcccgtg gccgcgtaga aatccgtcca agaggcaacg tgctgctctc cgcgtcgctc
     2641 tacgagtgag gtgaaatgtg ctcggcgttt atgccgagcg agtcgaagcg acgcgtcggc
     2701 agcgatggtc accacgatgt gtacctaagg ggttactgaa tccatattaa taatgcaatt
     2761 cccatgccga agggtccgcc ggggccctcc gttgtcgcgg tcccgcgacg ttttggaggg
     2821 cggtcgactg actggcaggc tgcgcgcagt ttttggttag agtaggagaa ccgaatgcgg
     2881 tggtggctca acactttgcc ggcgctcgcc ttgatcgttg cgggatcgag cgaagcggca
     2941 acgtactacg tgcgtaacgg cggcagcgat tccgccgacg gacgttcaca ttcgacggca
     3001 tgggcgaccc tgagcaaggt caacgcctac tcgtttgcgg cgggcgacgt cgtgctgttg
     3061 cacgaaggcg atcgcttcgt cggtcaggtc accgtcgatt gggccggtaa caccacggcg
     3121 cgctcggttt tgggcgccta ctatctcgac gcttcgagca acgccgtgcg cggctatcgc
     3181 tcggctcggc cgacgatcga cggcgaggat cgcctgccgg ctacgcgcta cgacgcgatg
     3241 gttacggtgc gcgcaaatcg cgtgcgcgtc gagaacttgc gcgttcagaa ttccgaagca
     3301 cgcggcatcg cgacgatcga atcgcaggac accgagatcg tcggttgcgc cgtttacaat
     3361 gtctacaacg cgggcgtgca tttcttgaaa tcgctgcgtt cgctggcaga aaacaatttc
     3421 gtgaccggcg cgggcatcgg caatcagcag gacggatcgc cgtggggggc gtcgatcgaa
     3481 tccgtggcga gccacgacac ggtcgtgcgt aacaacacga tcagcgaatc gtacggcgaa
     3541 gggatcaaca cgcacaccgg ctcgcagcgc acgctgatcg agcgcaacta tttgttcggc
     3601 gtgcgcgccg tcggtatcta tatcgacggt tcgcccgacg cgaccgtgcg gcgcaacatc
     3661 gtcgtcggca cgacgacgag cgcgtattgg cgcggcggca gcagcgtcgg atccggcatc
     3721 gtgctgaaca acgagtcgta tcactatccc gtcggcggcg gctcgctgag cacgagcgtg
     3781 caggcgaagc gggcgaaaat ctacggcaac ctggtgtcgt tcacgagcac gggcatcgcc
     3841 ttctg
//
LOCUS       DATLEE010000582         6225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3345833, whole genome shotgun sequence.
ACCESSION   DATLEE010000582 DATLEE010000000
VERSION     DATLEE010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3345833"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VM692_12625"
     CDS             <1..388
                     /locus_tag="VM692_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53061.1"
                     /translation="PARNPTSAATPPPAAPATATEPRAERRVRETEARVAPSAPSAPS
                     AGRSDADDEEETIIPIVVVNTRTIPNRETTFTTKDGKTWVQTDTQRLVNLPETPFEAE
                     IKSGAMGSYFLVAKDRPRAVRVRQVR"
     gene            complement(392..1489)
                     /locus_tag="VM692_12630"
     CDS             complement(392..1489)
                     /locus_tag="VM692_12630"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53062.1"
                     /translation="MSERVAVVFSMVCMALLATPAVAHHSFSAEFEADKTAELSGTID
                     QVWWNNPHIRYRIAVMGEGGATESWELQAASITAMQQLGWKQDTLKVGDALKVAGQVG
                     RNGAKKLYVRSIERPDGTRLVTGRGDATGPDPNKVNATPGKTYGYGAARNNYPVDITG
                     PWRNRYKWHVTVDDLEPKPTPFTAEGRALFAATEHYQDPALRCLALGLPRLFGAPYNM
                     DIVDAGTHYVQLYVEHNTPRRIWMDGRKPAPDTQPSSLGFSVGHWEGTTLVVETTNLL
                     PGWLDGSGLPMKGEGTRIVERYELSADKLSMDRIMTIYDPYYTQPLVRRRGSARDDAG
                     VDIAEQASCDPDSYYRDLLELGKLEEHLNGQ"
     gene            1932..3221
                     /locus_tag="VM692_12635"
     CDS             1932..3221
                     /locus_tag="VM692_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUQ53063.1"
                     /translation="MTAPFVQEALARHDLQLAAGSAWSLDQLTLAAWLLRTDLAVARS
                     EVAAARAATGVDTERPNPTVSTTTENIVDRGVEDPWVIGAALALTFELGHKRDIRAQR
                     AFAHEQALEWQFGQALWAARAEVRAALLDLAFAEQLAAFDTEEVRLTRAFLAWVETRL
                     ERGAATTSERLAALQAVNESESRRQIDNAAIAKASATLAATLGVAPQELARSPAALPP
                     LATLPTIAVEDVNAARALALVNRLDVRRALAEYEIAEQDLRAAVASQYPDLTLAPGYL
                     VDQADHKITLGLDLPVPLFHNASATIQGAIAGRTLAAAKFDDTQSSVLAAIDIGFAQY
                     QSSRSALAAAEQAERDAADAAASLERQLAAGAANRGELLAREIARTSLSRSALAARRA
                     LIDALTALENGVQRPLFPSSSLEPAAVIRELLAESSR"
     gene            3218..4183
                     /locus_tag="VM692_12640"
     CDS             3218..4183
                     /locus_tag="VM692_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53064.1"
                     /translation="MTAHRGRWVLCAVISLALAACNREPSSDTPLDSAEPEAAQLGLV
                     LSAEDREKLGVELDEVASATFQPTIDGPAYVVDAQAVVGAMAELGKAEADARTSQAAL
                     KRTRDLFNNDTAVSAEALEAAERAAATDDAQLRIARARATLAFGATAPWLDANRRDPL
                     LAALSGGSMLLISASFPSGLGDVRPDAITLRRVGNTGAPSWTATEIWAGPSDANVPGP
                     TLLALLDPPADLSYGERLRASAPIGATLSGVTVPVSAVVLAGGEAWCYVESADEELVR
                     RRVDLGRPVDFGYFHDQGFASAERIVTAGAGLLLARELGGGAEED"
     gene            4190..>6225
                     /locus_tag="VM692_12645"
     CDS             4190..>6225
                     /locus_tag="VM692_12645"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUQ53065.1"
                     /translation="MLQVISWCVRRRGVVAILAVAFLVLGSLAARRTPIDVFPEFVPA
                     QVSIQTEAPGLTPDQVETLITRPIEVAVNGVPALAALRSESIPGLSVVALDFRSGADP
                     QRAHQDVAERLASVASSLPAGTGPPKLSPLTSSTMDVLKIGLVSDTLDGFALRDLADW
                     SLKPQLLAVPGVARVNVFGGDVRQIEVTPNLAKLEAYGLGAADLAEAARAALVLRGAG
                     FVDLPAQRVLIESPVPAPDPAALAAAVVTVRNGVPLTLGDLASVETASALRAGDALVQ
                     GRAGVLLTISSQFGANTMDVTRALETALADIVPQLASDGITVYPALHRPANFIERALG
                     NLRDGLAIAAVLVLVVLFAFLRDVRAALISFITIPLSLIAATLVLGYFGFTLNTMTLG
                     GFAVSVGVLVDDAIIDIENILRRLRANAALATPLPRLEVVRNASLEIRSPVVFATLAV
                     VVLFVPVYFISGVQGLFIGPLALAFIAAVLASLVVAMTVTPALSALLLARGNVRPEAQ
                     WIDAIKRAQAKAAVWISERFALVAAVLLAAFAATLLWLPSLGGQFMPDFEEGHFVVQA
                     SSALPGTSFEEMLGVGRRISAELLALPYVATVEQQIGRAELGEDTWGPNRSEFHVELE
                     AEPGIDQSAAQAAIRDIVESYPGLRTEVVTFLGDRFSESLTGETAAVVVNVFGE"
BASE COUNT          960 a         2127 c         2082 g         1056 t
ORIGIN      
        1 gcccgcgcgc aacccgacgt cggccgccac gccgccgccg gccgcgcccg caactgcaac
       61 ggagccgcga gccgagcggc gcgtgcgtga gaccgaggcg cgcgtcgcac cgagtgcgcc
      121 gtcggcaccc tcggccggaa gaagcgatgc cgacgatgag gaagaaacga tcattcccat
      181 cgtcgtcgtc aacacgcgca cgattccgaa tcgcgagacg acgttcacga cgaaggacgg
      241 caagacgtgg gtccagacgg acacgcagcg actcgtcaac ttgcccgaga cgccgttcga
      301 ggccgagatc aaatccggcg cgatgggcag ctacttcctc gtcgcgaagg accggccgcg
      361 cgcggtccgc gtgcggcagg ttcgctgagc gctactgccc gttcaagtgc tcttctagct
      421 tgccgagctc gagcaaatcg cggtagtagc tgtcgggatc gcagctcgcc tgctcggcga
      481 tgtcgacgcc cgcgtcgtcg cgagccgagc cgcggcgccg cacgagcggt tgcgtgtagt
      541 acgggtcgta gatcgtcatg atgcgatcca tcgacagctt gtcggcgctg agctcgtaac
      601 gctcgacgat gcgcgtgccc tcgcccttca tcggcaggcc cgagccgtcg agccaacccg
      661 gcagcaaatt cgtcgtctcg acgaccagcg tagtgccttc ccaatgcccg accgagaaac
      721 cgagcgacga cggctgcgtg tcgggtgccg gtttccggcc gtccatccag atgcgccgcg
      781 gcgtgttgtg ctcgacatag agctgcacgt agtgcgtgcc ggcgtcgacg atgtccatgt
      841 tgtacggcgc gccgaacagc cgcggtaaac cgagcgcgag gcagcgcagc gcgggatcct
      901 gatagtgctc cgtcgccgcg aacagcgcgc ggccttcggc cgtgaacggc gtcggcttag
      961 gctcgagatc gtcgacggtc acgtgccact tgtaacgatt gcgccagggt cccgtgatgt
     1021 cgacgggata attgttgcgc gcggcgccgt agccgtaggt cttgccgggc gtcgcgttga
     1081 ccttgttggg gtccgggccc gtcgcgtcgc cgcggcccgt gacgagccgc gtcccgtcgg
     1141 gccgctcgat gctgcgcacg tacagcttct tggcaccgtt gcggcccact tggcccgcca
     1201 ccttgagcgc gtcgccgacc ttcaaggtgt cctgtttcca gccgagctgc tgcatggccg
     1261 tgatgctcgc cgcctgcagc tcccagctct cggtcgcacc gccctcgccc atgaccgcga
     1321 tgcgatagcg gatgtgcggg ttgttccacc agacctggtc gatcgtgccg gacagctcgg
     1381 cggtcttgtc ggcttcgaac tcggccgaga acgaatgatg cgcgactgcg ggcgttgcca
     1441 gcagcgccat gcagaccatc gaaaacacta cggcgacccg ctcgctcatg accaccccct
     1501 atctcgcaag agaaaaggat acagcggtcc cggcgccgcc tccatgcgag atcgatgcgg
     1561 ccggcaattt acggccggaa cgctgaccgt cggatgcgaa gccgaacccg cgcgaacggc
     1621 ccgtcgagaa cctcgggcgc tcgctgcgcg gtgccatgtc attagacggc gcgggcgcga
     1681 aaattggtcg ctcctgtgtg cttctctcgc agaactccgc gcacgcgtcg tgtctacgcg
     1741 gttgcgttgt atgatccgcg agaccattac acagcgtcgc gctgacacct tcgcgcatga
     1801 atcgacgaac gctacgtggg ctgcgccgga agatcggcac gttgtggctg gtagggcttg
     1861 tcgcttgccg gcacgttccg ccggcgccgc tcgacgccgc ggccaacgct gaacgacttg
     1921 ccgctcgctc tttgaccgcc cccttcgtgc aggaggcgct cgcgcgtcac gatttgcagc
     1981 tcgctgcggg cagcgcttgg tccctcgacc agctgacgct cgcagcatgg ttgctgcgca
     2041 cggacctcgc cgtcgcgcgc tccgaagtgg ctgcagcacg cgcagcaacc ggcgtcgata
     2101 ccgagcgccc gaatccgacc gtttcgacga ccaccgagaa catcgtcgat cgcggcgtcg
     2161 aagacccatg ggtaatcggc gcggccctcg cgctgacttt cgagctcggg cacaaacgcg
     2221 acattcgcgc gcagcgcgca ttcgcgcacg agcaggccct cgagtggcag tttggccagg
     2281 cactctgggc ggcgcgcgcc gaagttcgcg ccgcgctgct cgatttggca ttcgcggagc
     2341 agctcgcggc gttcgacacg gaggaagtcc gtctcacgcg cgcgtttctc gcctgggtag
     2401 agacgcgact cgagcgcggc gccgccacga cttcggagcg ccttgcggcc ttacaggccg
     2461 tgaacgaaag cgagagccgc cgccagatcg acaacgctgc aattgccaaa gcatccgcaa
     2521 cgcttgccgc cacgctcggt gtggcgccgc aggagctagc gagatcgccc gctgccctgc
     2581 cgccgttggc aaccttaccg acgatcgccg tcgaggacgt gaacgccgct cgcgcactcg
     2641 cgctcgtcaa ccgcctcgac gtgcgacgcg cgctcgcgga atacgagatc gccgagcagg
     2701 atctgcgcgc ggcagttgcc tcgcagtatc ccgacctcac gctggcaccg ggatacttgg
     2761 tcgaccaagc cgatcataag atcacgctcg gtctcgatct tcccgtaccg ctgtttcaca
     2821 acgcgagcgc gacgatccag ggtgccatcg ccgggcgcac gctggccgcg gccaagttcg
     2881 acgacacgca gtcgagcgtg ctcgcagcga tcgacatagg cttcgctcaa tatcagtcgt
     2941 cgcgcagcgc gctcgccgcc gccgagcaag cagagcgcga cgcggccgac gcggcagcgt
     3001 cgctcgagcg gcaactcgca gccggagccg cgaatcgcgg cgagctgctc gcgcgcgaga
     3061 tcgctcgcac gagcctgagc cgcagcgcac tcgctgcgcg tcgcgcactg atcgacgcgc
     3121 tgacggcgct cgagaacggc gttcagcgcc cgctattccc gtcatcgtcg ctcgagcccg
     3181 cggccgtgat ccgcgagctg ctcgccgagt cttcgcgatg acggcgcacc gcgggcgttg
     3241 ggtgctgtgc gcggtcattt cgttggcgct cgccgcatgc aatcgcgagc cgtcgtcgga
     3301 cacgcccctc gacagcgccg agccggaggc cgcgcagctc gggctcgttt tgagcgccga
     3361 ggaccgcgag aaactcggtg tggagctaga cgaagtcgcg agtgcaacgt ttcagccgac
     3421 catcgacggg cctgcttacg tggtcgacgc acaagcggtg gttggggcga tggccgagct
     3481 cggtaaagcc gaggcggatg ccaggacgag ccaagccgcg ttgaaacgca cgcgcgacct
     3541 cttcaacaac gatacggccg tctctgcgga ggctctggag gcggcggagc gtgcagcggc
     3601 gacggacgat gcgcagcttc gcatagcgcg cgcgcgcgcc acgctcgctt ttggcgcgac
     3661 ggcaccatgg cttgacgcga accgtcgcga cccgttgctc gcggcgctgt cgggcggctc
     3721 gatgctgctc ataagcgcaa gcttcccgtc cggactcggc gacgtgcggc ctgacgcgat
     3781 cacgctacgc agggtcggca acaccggcgc gccgagctgg accgcgacag aaatctgggc
     3841 cggaccctcc gatgccaacg tcccggggcc gacgttgctc gcgttgctcg acccaccggc
     3901 cgacctgagc tacggcgaac gcctgagagc gagcgcgccg atcggcgcta cgttgtccgg
     3961 cgtgaccgtg ccggtcagcg ccgtggtgct cgcgggcgga gaagcatggt gctatgtcga
     4021 gtcggccgac gaggagctcg tgcgccgacg cgtcgatctc ggccggccgg tcgacttcgg
     4081 atacttccac gatcaaggct ttgcgtccgc cgagcgcatc gttacggctg gggctgggct
     4141 cttgctcgct cgcgagctcg gcggcggcgc cgaggaagac tgagcgctga tgctgcaggt
     4201 catttcctgg tgcgtgcggc ggcgcggtgt cgtcgcaatt cttgccgtcg ctttcctagt
     4261 gctcggctct ttggccgcac gacgaacgcc gatcgacgta ttcccggaat tcgtcccggc
     4321 gcaggtctcg atccagaccg aggcgcccgg gctcacgccc gaccaagtcg agacgctgat
     4381 cacgcggccc atcgaggtgg cagtcaacgg agtgccggcg ctcgcggcgc tgcgctccga
     4441 atcgatcccg ggcctgtccg tcgtcgcgct cgattttcgg tcgggcgccg acccgcaacg
     4501 cgcacatcag gacgttgccg aacggctcgc gagcgtcgca tcgtcgctac ctgccgggac
     4561 gggtcccccg aagctttcgc cgctcacgtc gagcacgatg gacgtgctga aaatcggtct
     4621 cgtctccgac actctcgacg gcttcgcgct gcgcgacctc gccgactgga gcttgaaacc
     4681 gcagctgctc gccgtgcccg gcgtcgcacg cgtcaacgta ttcggcggcg acgtgcgcca
     4741 gatcgaggtc acaccgaatc tcgccaagct cgaagcctac ggtctcggcg cggccgatct
     4801 cgctgaggcg gcccgcgcag cgctcgtgtt gcgcggcgcg ggattcgtcg acctgcccgc
     4861 acagcgcgtc ttgatcgaat cgcccgtgcc ggcgcccgac cccgcggcac tcgccgcggc
     4921 cgtcgttacg gtgcgcaacg gcgtgccgct cacgctcggc gacctcgcca gcgtcgaaac
     4981 ggcatccgct ctgcgtgcag gcgatgcgct cgttcaaggc cgcgccggcg tgcttctgac
     5041 gatctcgagc cagttcggcg cgaacaccat ggacgtgacg cgcgcgctcg aaacggcgct
     5101 tgcggacatc gtgccgcaat tggcaagcga cggcatcacg gtgtatcccg cattgcatcg
     5161 cccggccaac ttcatcgagc gcgcgctcgg taatctgcgt gacgggctcg cgatcgcagc
     5221 ggtgctggtg ctggtcgtgc tgttcgcgtt cctgcgcgac gtgcgcgcag cgctcatttc
     5281 gttcatcacg attccgctgt cgctgatcgc agccaccctc gtgctcggct acttcgggtt
     5341 tacgttgaac acgatgacgc tcggcggttt cgccgtgtcc gtgggcgtgc tcgtcgacga
     5401 cgcgatcatc gacatcgaga acatcctgcg gcgtctgcgc gcgaacgcgg cgcttgcgac
     5461 gcctttgccg cggctcgagg tggtacgcaa cgcgtcactc gagatccgct ctcccgtcgt
     5521 gttcgcaacg ctcgcggtcg tcgtgctgtt cgtgcccgtg tatttcatct cgggcgtgca
     5581 gggcttgttc atcggcccgc tggcgctggc attcatcgca gccgtcctcg catcgctcgt
     5641 cgtggctatg acggtgacgc cggcgctgtc ggctctgctg cttgcgcggg gcaatgtccg
     5701 cccggaggct caatggatcg atgcgatcaa gcgtgcgcag gcgaaggccg cagtgtggat
     5761 cagcgagcgt ttcgcactcg tggcggcagt gctccttgca gccttcgctg cgacgctgct
     5821 atggctgccg tcgctgggcg gccagttcat gcccgacttc gaggaaggcc atttcgtggt
     5881 gcaggcgagc agcgcgttgc ccggcacgtc gttcgaagag atgctcggtg tcgggcggcg
     5941 gatcagcgcg gagcttttgg cactgccgta cgtggcgacc gtagagcaac agatcgggcg
     6001 ggccgagctc ggcgaggaca cctggggtcc gaaccgcagc gagtttcacg tcgagctcga
     6061 agcggagccc ggcatcgatc aaagcgccgc gcaggcggcg atccgcgaca tcgtcgaaag
     6121 ttaccccggg ctccgcacgg aggtcgtgac gttcctcggc gaccgattca gcgagagctt
     6181 gacgggcgag accgcggcgg tcgtcgttaa cgtgtttggt gaaga
//
LOCUS       DATLEE010000583         3612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9641844, whole genome shotgun sequence.
ACCESSION   DATLEE010000583 DATLEE010000000
VERSION     DATLEE010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9641844"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VM692_12650"
     CDS             <1..338
                     /locus_tag="VM692_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53066.1"
                     /translation="LACFGVPGKTPAGRDERRETRGDRETALQLERRREALARQETGR
                     DQLFADAPSTDALSVERRAEVGVRDLARRDENLAESQLDALRGVAAPRDSSECRVLHR
                     ARHRFTRSF"
     gene            546..>3612
                     /locus_tag="VM692_12655"
     CDS             546..>3612
                     /locus_tag="VM692_12655"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HUQ53067.1"
                     /translation="MAIKHGKQGNDTIVGTAQHDKLFGQHGDDILWGAGGDDDLDGGH
                     GFDIARYSGSFFDYRITADKHGDYTVADLRAGRPDGVDSLRKVEALQFSDRIIYIDGR
                     NNAPVTVNDSASGSEDIAVRIAASTLLANDRDFDGDALALVSVGGATHGSVSLANGIV
                     TFTPAASYNGAAAFNYTVRDARGAQSTGTVNLSIQAVNDAPVNTVPGAKSVDEDTTLT
                     IGGISVSDVDAATVRVTLSAAHGALSLSRTSGLTFTSGDGVSDATMTFSGTQANVNAA
                     LGNLSYRPTANYNGADAIRLTTTDLGGTGTGGALSDTDTINVTVRPVNDAPVAATDAR
                     SVNEDTRLTAASVLANDSDVDGNTLTARLVSGPAHGSLTLNADGTFVYTPVANFNGAD
                     SFTYRAFDGTTTSNPATVSIAVKSVNDAPTFFGSHGRVIVDFGQGQETGRAIAIQPNG
                     KIVIAGEAAGGMGGARFNTDGSADSTFDGDGQTLVAFPLFDGVPFGMALQANGRIAIA
                     GYGSNSNEIDFALARLNANGSLDATFDGDGRVMTGFTPGSVDIAHAVAVQADGKLVAA
                     GGSSFYDFSVVRYNANGSLDTTFSGDGRAITALSADPDYANAVAVQSNGKIVAAGFRN
                     NGAQDDFALVRYNADGSLDTTFDGDGIVITDFSGGNDVITGIAVQSDGRILVSGYSES
                     TPGQTVFAAACYNANGTLDTAFDGDGKLTTGFDGLNSAANAVALQANGKFVLAGAAAS
                     NIAIARYNADGSLDTSFDGDGRVISDAGSVANAVALQADGGIVIGGALHAQMVVARFN
                     ANGSLDTTFGRGNTLDASPTFIEDQAPVVLDSDVLVFDAELAAQGHYAGARVTLVRHG
                     GANAEDVYSNAGTLGLLAQGSALVYDGVNVGTVTTNSGGTLVLAFNSGATQDRVNGVL
                     QAIAYSNSSDTPPASVQIDWSFSDGNTGAQGAGGALSATGSTTVAILSSLDRPIAAND
                     SYATDEGVPLLGSSVLANDHDPDGDTLTAVLASGPSHGSLVLNDDGTFTY"
BASE COUNT          660 a         1221 c         1185 g          546 t
ORIGIN      
        1 gtctggcctg cttcggcgtg cccggaaaaa ccccggctgg tcgggatgag cgccgcgaga
       61 cacgcggcga tcgcgagacg gcgctgcagc ttgagcgcag gcgcgaggcg ctcgcgcggc
      121 aggagaccgg ccgcgaccag ctcttcgccg atgcgccttc cactgacgcg ctttcggtcg
      181 agcgccgcgc cgaggtcgga gtccgagatc tcgcccgtcg cgacgagaat ctcgccgagt
      241 cgcagcttga cgctctccgg ggcgtggccg cgccgcgcga ttctagcgaa tgtcgcgtgc
      301 tccaccgcgc tcgtcatcga tttacccgtt ccttttgagc tcgcgtcccg gcgcgtgcaa
      361 taagtcacgc attgactcaa tacctgtcca ccattaaaca ggacgtagag ggataacggc
      421 agcgcttgcg agatcgagca gggctctcat cgcgcaaacc tcacggaacg tgagatttgt
      481 cgcggatgca catcactgca taagctcgcc gccgacgctg atcacaacta gatacagggg
      541 gaatcgtggc catcaaacac ggcaagcagg gcaacgacac gatcgtcggc accgcgcagc
      601 atgacaagct cttcgggcag cacggcgacg acattctgtg gggcgccgga ggtgacgacg
      661 acctcgacgg cggccacggc ttcgacatcg cgcgctacag cggctcgttc ttcgattacc
      721 gcatcaccgc ggacaagcac ggcgactaca cggtcgcaga tcttcgcgcc ggccgccccg
      781 acggcgtcga ttcgctccgc aaggtggaag cgctccagtt ctccgatcgc atcatctaca
      841 tcgacgggcg caacaatgcg ccggtgactg tgaacgattc ggcttcgggc agcgaagaca
      901 tcgccgtcag gatcgctgca tcgacgctgc ttgcgaacga ccgcgatttc gacggcgacg
      961 cgctcgcgct ggtgtcggtc ggcggcgcga cgcacggttc ggtgagcctc gcgaacggca
     1021 tcgtcacctt cacgccggcc gcgagctaca acggcgcggc agccttcaac tacaccgtgc
     1081 gcgatgcgcg cggcgcgcag agcaccggca cggtgaatct ctcgatacag gccgtgaacg
     1141 atgcgcccgt caacacggtg ccgggcgcga agagcgtcga cgaagacacg acgctcacga
     1201 tcggcggcat cagtgtttcg gacgtcgacg ccgcgacggt tcgagtcacg ctctcggccg
     1261 cacatggcgc gctgagcctt tcgcggacga gcggtctcac gtttacgtcg ggcgacggcg
     1321 tctcggacgc gacgatgacg ttcagcggca cgcaggcgaa cgtcaatgcg gcgctcggca
     1381 atctcagcta ccgcccgacg gcgaattaca acggcgcgga cgcgatccgg ctcacgacga
     1441 ccgatctcgg cggcaccggc acgggcggcg cgctgagcga caccgacacg atcaacgtca
     1501 cggtgcgccc ggtcaacgac gcaccggtcg cggcgaccga cgcacgcagc gtgaacgagg
     1561 acacgcggct caccgcggcg agcgtcctcg cgaacgacag cgacgtcgac ggcaacacgc
     1621 tgaccgcgag gctcgtctcg ggtcccgcgc acggttcgct caccctcaac gcggacggca
     1681 cgttcgtcta tacgcccgtc gcgaacttca acggcgccga cagcttcacc tatcgtgctt
     1741 tcgacggcac gaccacgagc aatcccgcga cggtgtcgat cgcagtcaag tccgtcaacg
     1801 atgcaccgac gttcttcggc agccatggca gggtgatcgt cgacttcggc caggggcagg
     1861 agacaggccg cgcgatagcg atccagccga acggcaagat cgtgatcgcg ggggaagcgg
     1921 cgggcgggat gggtggcgcg cgcttcaaca ccgacggcag tgcggactcg acgttcgacg
     1981 gtgacggcca gacgctcgtc gcgttcccgc tgttcgacgg cgtgccgttc ggcatggcgc
     2041 tccaggcgaa cggcaggatc gccatcgccg gctacgggtc aaacagcaac gaaatcgatt
     2101 tcgcgctcgc tcggctgaac gcgaacggca gtctcgacgc cacgttcgac ggcgacgggc
     2161 gcgtcatgac cggattcacg ccgggcagcg tcgacatcgc gcacgccgtc gcggtgcagg
     2221 ccgacggcaa gctcgtcgca gccggcggca gctcgttcta cgacttcagc gtcgtgcgct
     2281 acaacgcgaa cggcagtttg gacacgacgt tcagcggcga cggcagggcc atcacggcgc
     2341 tcagcgccga ccccgattac gcaaacgcgg tcgccgtgca gtcgaacggc aagatcgtcg
     2401 cggcaggttt tcgcaacaac ggcgcccagg acgatttcgc cctcgtgcgc tacaacgccg
     2461 acggcagcct cgatacgacc ttcgacggcg acggcatcgt catcaccgat ttctcgggcg
     2521 gcaacgatgt catcacaggc atcgccgtgc agtcggacgg caggatcctg gtctcaggct
     2581 actcggagag cacgccgggc cagacggtct tcgcggctgc gtgttacaac gccaacggca
     2641 cgcttgacac ggcgttcgac ggcgacggca agctgaccac cggtttcgat ggcttgaaca
     2701 gcgcggcgaa tgccgttgcg cttcaggcga acggcaagtt cgtgctcgcc ggcgccgcgg
     2761 cgtcgaacat cgcgatcgca cgttacaacg ccgatggaag tctcgacacg agcttcgacg
     2821 gggacggcag agtgatcagc gacgccggca gcgtcgcgaa cgcggtcgca ctgcaggcgg
     2881 acggcggcat cgtcatcgga ggtgcgctgc acgcgcagat ggtcgtcgcc cgcttcaatg
     2941 cgaacggcag cctcgacacc acgttcggcc gcggcaacac tctcgacgct tcgccgacct
     3001 tcatcgaaga ccaggcgccg gtcgtgctcg acagcgacgt gctggtgttc gacgcggagc
     3061 tcgcggcgca ggggcactat gccggagcgc gagtcacgct cgtgcgtcat ggcggcgcga
     3121 atgcggaaga cgtgtattcg aatgcgggca cgctcggcct gctcgcgcaa ggctcggcgc
     3181 tcgtctacga cggtgtcaac gtcggcacgg tgacgacgaa ttccggcggc acgctcgttc
     3241 tcgcgttcaa ctccggcgcg acgcaggacc gagtgaatgg agtgcttcag gcgatcgcat
     3301 actcgaacag cagcgacacg ccgcctgcgt cggtgcagat cgactggagc ttctccgacg
     3361 gcaacaccgg cgcgcaaggc gcggggggcg cactctcggc aacgggcagc accacggtcg
     3421 ccatcctttc gtcactcgat cgcccgatcg ccgccaacga ttcgtacgcg accgacgagg
     3481 gcgtcccgct cctgggctcg agcgtgctcg cgaacgacca cgacccggac ggcgacacgc
     3541 tgacggcggt gcttgcgtca ggcccttcgc acggctccct cgtgctaaac gacgacggca
     3601 cgtttaccta tg
//
LOCUS       DATLEE010000584         6363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6310030, whole genome shotgun sequence.
ACCESSION   DATLEE010000584 DATLEE010000000
VERSION     DATLEE010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6310030"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VM692_12660"
     CDS             <1..684
                     /locus_tag="VM692_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUQ53068.1"
                     /translation="YVDGAPGTMSFDDLQVESPVGREGFQEQHVVLRRGEHTLICGAP
                     GEGKTPLFRALSGLWPWGSGRIEKPRDESVMYLPRGTPYLPRGTLREVLAYPLLTDRF
                     GDGDYVRALERTGLGRLVKSLDATERWDRALREDEQMALAFARLVLHTPDWVIFDDTF
                     SAMEDEALERVIDLFADDLARTTIIHIGRSTQAHLPLFSRVLHLKRLQSPTAEQQQWA
                     AERLLRRRR"
     gene            681..2948
                     /locus_tag="VM692_12665"
     CDS             681..2948
                     /locus_tag="VM692_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HUQ53069.1"
                     /translation="MNARVCTMAALLVTAIAARPFVVYAQDFGFEVPAAPTDSSLPAA
                     LRDLAERVLPGYEEDDPDRYLANLAALQMTIEDPTAAHATRLKLRERRESEPATRPAG
                     RAAVYDIYVHARAIEASDSVSFTAAYRQAFEEAMSRLDDVDAYELEPWFSTPAGAFEE
                     DLQRLLDSRREASSVTLGEALELVQASFAFDAYRSFGAEVRPLLAQDSARRYAVEEVV
                     IPVAPDATLAATLVRPRKALEAGEALPTLLEFTLDRAGSDAREAAAHGYASLLALARI
                     AGNAKSRPRAPFESDGDDARAVIDWIVQQSWTDGRVAMQGNRYGGFVAWSAAKRRPAA
                     LQAIATSDPLAPGIDVPSPNRIFHNSAYRWVQNLLTAAGGAAAREDSRSPAADDDWYR
                     TGRSYRELPPSSGRAASIFRSWLNHPSYDRFWQKWLPFGPEFDAIDIPVLTITGYYSA
                     GETAALYYFTQHRQHDEAAEHALLIGPFDEHSVVSGAAASLRDLDIDVVARIDAADAR
                     YAWLAHVLRGAERPELVSAAVNYELSGANEWRHESSLTALESNPLRFYLEAMPTGAAH
                     RLVAEQPAAPLALTTTVDLRDRDDFEWRPTRALVLDGLHAENGAIFATEPFGEDVDIA
                     GRFGGVLDFTVNKYDVDFVMTLYELNAAGVYVKLFDPGYAFRASYARNRTQRRLLVNG
                     VRQQVSFQSERMFGRRLAAGSRLVMALGVNKRADQQINYGAGGDVSEESLEDAGAPVR
                     VRWHEGTFIEVPAQR"
     gene            complement(2957..3169)
                     /locus_tag="VM692_12670"
     CDS             complement(2957..3169)
                     /locus_tag="VM692_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53070.1"
                     /translation="MNKADSFVRPDETSPRLDGDEITTECYEGVNLAPSTVASLVTWD
                     VSGLARHEQERLLAASRAPKDIQGGY"
     gene            3422..4945
                     /locus_tag="VM692_12675"
     CDS             3422..4945
                     /locus_tag="VM692_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HUQ53071.1"
                     /translation="MVCSLLAARTARALLAITLVASAPVALAFDFDDVATLARRKAAA
                     PYQAPDRAQPKELELLGYDRYRDIRFRPDRAIWRAEGLPFDLMFFHRAQSNPRVRMNA
                     VVDDKARHLPYDSDDFDFGKNTLSPEEWGELDYAGLRVHYHLNGPQYKDELIVFLGAS
                     YFRALAAGTRYGLSARGLAVDTIGGQGEEFPYFPEFWIVKPEPDARTLRMFALLESER
                     VSGAFQFDVTPGDETVVEVHSRLYLRGSINTLGIAPLTSMYQFGENQPHRVDFRPEVH
                     DSDGLMVATGDGEWLWRPLINPNSALTTSFSMREVRGFGLMQRDRSFTSYEDPETRYD
                     LRPSAWVEPLGSWGAGRVELVQLHTPNETNDNIVAYWVPERLPAPGEPVDFSYRLHWQ
                     GARLAQQPPGGRVIQSRVGRGYRELADDQQQFMVDFMGPTLAALPPDAPVKAVVSAPV
                     NGEILETNAYHVEATGAWRMMVLVKQLDTTQALELRGYLQNGADVLTETWSNVVPPR"
     gene            5081..6139
                     /locus_tag="VM692_12680"
     CDS             5081..6139
                     /locus_tag="VM692_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HUQ53072.1"
                     /translation="MFVRGLGTAHPARRYTKAECLTAFQASSWFDKLDRRARTIAEVV
                     LLGDNGINARHLALDSLEDVFAIDPDTLHARFLAHAPQLAADAGAKALLDANIEPGEI
                     DAIVVSTCTGYLCPGLSGYVVERLGLRANLAAFDLVGHGCAAALPNWQLADALLASRR
                     CQNVLSVCVEVSSAALYLDNDPGVLVSACLFGDGAGATVLSNEANPDKRSIECKTIGS
                     LLNTGERAALRFEQRSGMLRNVLTRPVPKLAAAHAQRVLDDVLGRSGIGRDDIAAWIW
                     HAGGRDVLLALRSQLGLTEEQTRYSAATLREFGNLSSAFVYFVLEAALADRARGGWWW
                     MSSFGAGFSCHGALLNVA"
     gene            6136..>6363
                     /locus_tag="VM692_12685"
     CDS             6136..>6363
                     /locus_tag="VM692_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53073.1"
                     /translation="MSTKVATADAAPRWPRRVLPELLDELAPNDPRARRSRRDLRLVN
                     RIMGHPLLLARALDAVVTRAPLRLVELGAGDG"
BASE COUNT         1029 a         2173 c         2135 g         1026 t
ORIGIN      
        1 tacgtcgacg gggcgcccgg caccatgtcg ttcgacgacc tgcaagtcga atcgcccgtc
       61 gggcgcgagg ggttccagga gcaacacgtc gtgctgcgcc gcggcgagca cacattgatc
      121 tgcggcgcac ccggcgaggg caagacgccg ctgtttcgcg cgctgtcggg gctttggccg
      181 tggggcagcg gccgcatcga gaagccgcgc gacgagtccg tgatgtatct gccgcgcggc
      241 accccgtatc tgccgcgcgg cacgctgcgc gaagtgctcg cttatccgct gctcacggac
      301 cgcttcggcg acggcgacta cgtgcgtgcg ctcgagcgca cgggtctcgg gcggctcgtg
      361 aagagcctcg acgcaacgga acgctgggac cgcgcgctgc gcgaggacga gcagatggcg
      421 ttggcattcg cgcgcctcgt gctgcacaca ccggactggg tcatattcga cgacacgttc
      481 tcggcgatgg aggacgaggc gctcgaacgc gtcatcgatc tcttcgcaga cgacctcgcg
      541 cggacgacga tcattcacat cggccgaagc acccaggcgc acctgccgct gttctcgcgc
      601 gtgctgcact tgaagaggct gcagagcccg accgccgagc aacaacagtg ggcggccgag
      661 cggctgctac ggcggcggcg atgaacgccc gcgtgtgcac gatggccgcg ctgctcgtaa
      721 cggcgatcgc tgcgcggccg ttcgtcgtgt atgcgcagga cttcggcttc gaggtgccag
      781 cggcgcccac ggactcgtcg ctgccggccg cgttacgcga tctcgccgag cgtgtcctgc
      841 ctgggtacga ggaggacgat cccgatcgct accttgcgaa cctcgcggcg ctgcagatga
      901 cgatcgagga tccgaccgcg gcacatgcga cgcggctgaa gctgcgagag cgccgcgaga
      961 gcgagccggc cacgcggccg gccggtcgtg cggcggtcta tgacatctac gtgcacgcac
     1021 gggccatcga agcgagcgac agcgtgtctt tcacggccgc ctaccggcag gcgttcgaag
     1081 aagcaatgag tcgcctcgac gacgtcgatg cctacgagct cgagccctgg ttcagcacac
     1141 cggccggcgc tttcgaggag gacttgcagc gcctgctcga cagtcggcgc gaagcgagct
     1201 ccgtgacgct cggcgaggca ctcgagctcg tgcaagcctc gttcgcgttc gatgcctatc
     1261 gcagcttcgg cgcggaggtg cggccgttgc tagcccagga ttcggcgcgc cggtacgccg
     1321 tcgaagaagt cgtgattccc gttgcgcctg atgcgacgct cgcggcgacc ttggttcggc
     1381 cgcgcaaggc tctcgaagcc ggcgaggcgc tgccgacgct gctcgagttc acgctcgatc
     1441 gagccggctc ggatgcgcgc gaggccgcgg cgcacggcta cgcgagcctg ctcgcgctcg
     1501 cgcgcattgc cggcaacgcc aagtcgcggc cgcgcgcgcc gttcgaatcc gacggcgacg
     1561 atgcgcgcgc cgtcatcgat tggatcgtgc aacagtcgtg gaccgacggc cgcgtcgcca
     1621 tgcaaggcaa ccgctacggg gggttcgtgg catggtcggc cgcgaagcgc cgcccggcag
     1681 cgctgcaagc gatcgcgacc tccgacccgc tcgcgcccgg tatcgacgtg ccgagcccca
     1741 acaggatctt ccacaactcc gcatatcggt gggtgcagaa cctcctgaca gcggccggcg
     1801 gcgcagccgc gcgcgaggat agtcgctcgc ctgccgccga cgacgactgg tacagaacgg
     1861 gccgcagcta ccgcgagctg ccgccgtcgt cgggccgtgc ggcctcgatc tttcgcagct
     1921 ggctgaatca cccgagttac gacaggttct ggcagaaatg gctgccattc ggccccgagt
     1981 ttgacgcgat cgacataccg gtgctgacga tcacgggcta ttactccgcc ggcgagacag
     2041 cagcgctgta ctacttcacg cagcatcggc aacacgacga agccgccgag cacgcactgc
     2101 ttatcggccc gttcgacgag cacagcgtcg tgagcggcgc cgcagcctcg ctgcgcgacc
     2161 tcgacatcga cgttgtcgcg cggatcgacg cggccgacgc gcgctacgcg tggctcgcgc
     2221 acgtgctgcg cggcgcggag cggcccgagc tcgtgagcgc cgccgtcaac tacgagctct
     2281 ccggcgccaa cgagtggcgc cacgagtcgt cgctgacggc gctcgaaagc aacccgctgc
     2341 gcttctacct cgaggcgatg ccgaccggcg cagcgcacag gctcgtcgcc gagcagcccg
     2401 cagcaccgct ggctctgacg acgactgtgg atctacgcga ccgcgacgac ttcgagtggc
     2461 ggccgacgcg cgccctcgtt ctcgatggat tgcacgccga gaatggcgcg atattcgcga
     2521 cggaaccgtt cggcgaggac gtcgatatcg caggccggtt cggcggcgtg ctcgatttca
     2581 cggtcaacaa gtacgacgtc gacttcgtca tgacgttgta cgagctcaac gcggccggcg
     2641 tgtacgtgaa gctgttcgat cccggctatg ctttccgcgc gagctacgcg cgcaaccgca
     2701 cgcaacgccg cttgctcgtc aacggtgtgc ggcagcaggt ctcgtttcag agcgaacgca
     2761 tgttcggccg cagattggcg gccggcagcc gtttggtcat ggcgctcgga gtcaacaagc
     2821 gcgccgatca acagatcaac tacggtgccg gcggcgacgt cagcgaggaa tcgctcgagg
     2881 acgccggcgc tccggttcgc gtccgctggc acgaaggaac cttcatcgag gtccccgcgc
     2941 agcgctgacc ggtgcgctag tacccgccct gaatgtcttt cggggcccga gaggccgcaa
     3001 gcagccgctc ttgctcgtgc cgcgcgaggc cggagacgtc ccacgtgacg aggctcgcga
     3061 ccgtgctcgg cgcgagatta acgccttcgt aacactccgt cgtgatctcg tcgccgtcga
     3121 gtcgtggcga ggtctcgtca ggtctcacga aagagtccgc tttgttcatc gtcagcctcg
     3181 ggggttttga attgttagcg acgcagacgg gcgtgcagta actgtgccac gtctgcacac
     3241 ggcgcgattg ctgcgcggcg acgaagccga cgcgcccgtt cgctggaacg aaattccaag
     3301 atttgatgaa gcgaagccgc tcggcggcca aacgcgaagt tctatcggcc gctatagccc
     3361 ggaccgcggt cagcgggcta tgttctacca ggaacaaagc gcacgaggca gattcaggat
     3421 catggtttgt tcgctcctcg cggcacgcac cgcgcgcgcc ttgctcgcaa tcacgttggt
     3481 ggcgagcgcg cccgtagcgc tcgcgttcga tttcgacgat gtcgccacac tcgcgcggcg
     3541 caaggcagcg gcgccgtacc aagcgccgga tcgcgcgcag cccaaagagc tcgagctctt
     3601 gggctacgat cgttaccgcg acattcgctt ccgccccgat cgcgcgatct ggcgagccga
     3661 aggtttgccg ttcgatctca tgttcttcca tcgcgcgcag tcgaacccgc gcgtgcgcat
     3721 gaatgccgtc gtcgacgaca aggcgcggca cctgccttac gacagcgacg acttcgactt
     3781 cggcaagaac acgctgtcac ccgaggagtg gggcgagctc gactacgcgg ggctgcgcgt
     3841 gcactaccat ttgaatgggc cgcagtacaa agacgagctg atcgtcttct tgggcgcgag
     3901 ctacttccgc gcgctcgcgg ccggcacgcg ctacggcttg tcggcgcgcg gcctcgccgt
     3961 cgacacgatc ggcggccaag gcgaggagtt cccgtacttt cccgagttct ggatcgtgaa
     4021 acccgagccg gatgcacgca cgctgcgcat gttcgcgctg ctcgaatccg agcgcgtcag
     4081 cggcgccttt cagttcgacg tcacaccggg cgacgagacc gtcgttgaag tgcattcacg
     4141 cctgtatctg cgcggctcga tcaacacgct cggcatcgcg ccgctgacga gcatgtatca
     4201 gttcggcgag aaccaaccgc atcgcgtcga cttcaggccc gaggtccacg actccgacgg
     4261 cctcatggtg gcgaccggcg acggcgagtg gctctggcgg ccgctgatca acccgaattc
     4321 ggccttgacg acctcgttct cgatgcgcga ggtcagaggg ttcgggctga tgcagcgcga
     4381 ccgcagcttc acgagctacg aggaccccga gacgcgctac gacttgcgac cgagcgcgtg
     4441 ggtcgagccg ctcggctcgt ggggcgcggg gcgcgtcgag ctcgtgcagc tgcacacgcc
     4501 gaacgagacc aacgacaaca tcgtcgcgta ctgggtgccc gaacggctgc cggcgcccgg
     4561 tgagccagtc gacttttctt accgcctgca ctggcagggc gcgcgactcg cacagcagcc
     4621 gcccggaggc cgtgtcatac agtcacgcgt gggccgcggt tatcgcgagc tcgccgacga
     4681 ccagcagcag ttcatggtcg acttcatggg cccgacgctc gcagcgctac cgccggacgc
     4741 gcccgtcaaa gccgtcgtct cggcgcccgt caacggcgag atcctcgaga cgaatgccta
     4801 ccacgtcgag gccacgggcg cttggcgcat gatggtgctc gtcaagcagc tcgacacgac
     4861 gcaagcgctg gagctgcgcg gctatctaca gaacggcgcc gacgtgttga cggagacctg
     4921 gagcaacgtc gtgccgccgc gctgattcga ggcacgcgtc gtcaagcagt gaccgtgaac
     4981 cgccggcgcg gtccggcggc actcatgtag tcgaccggga gcggcgcccg gcgctggcac
     5041 gatttcggca taccgacccg tgaccactgg aggacgcgct atgttcgtac gtgggttggg
     5101 cacggcgcat ccggcacggc gttacaccaa agccgagtgc ctcacggcgt ttcaggcgtc
     5161 gtcctggttc gacaagctcg atcggcgcgc gcgcacgatc gcggaggtcg tgctgctcgg
     5221 agacaacggc atcaatgcgc gccacctggc gctcgattcg ctcgaggacg tcttcgcgat
     5281 cgatcccgac acgctgcacg cgcgttttct cgcgcatgcg ccgcagctcg cggccgatgc
     5341 cggcgcgaag gcactgctcg acgcgaacat cgagcccggc gagatcgacg cgatcgtcgt
     5401 gagcacgtgc acgggctatc tgtgccccgg cttgagcggc tacgtcgtcg agcggctcgg
     5461 cctgcgcgca aacctcgcag cgttcgatct cgtcggccac ggctgcgccg cggcactacc
     5521 gaactggcag ctcgcggacg cgctgctcgc ttcgcgccgc tgccaaaacg tgctgtccgt
     5581 atgcgtcgag gtcagcagcg cggcgctgta tctcgacaac gaccccggcg ttctcgtcag
     5641 cgcctgcttg ttcggcgacg gcgccggcgc gacggtgctc tcgaacgaag ccaaccccga
     5701 caaacgctcg atcgagtgca aaacgatcgg ctcgctgttg aacacgggcg agcgtgcagc
     5761 gctgcgcttc gagcagcgca gcgggatgct gcgcaacgtc ctgactcggc ccgtaccgaa
     5821 gctcgccgcg gctcacgctc agcgcgtgct cgacgacgtg ctcgggcgct ccggcatcgg
     5881 ccgcgacgac atcgccgcgt ggatctggca cgccggcggc cgcgacgtgc tgctagcgct
     5941 gcggagtcag ctcggtctca ccgaagagca gactcgctac agcgcggcaa cgctgcgcga
     6001 gttcggcaac ttgagcagcg cgttcgtcta tttcgtgctt gaagctgcgc tcgcggatcg
     6061 cgcgcgcggc ggctggtggt ggatgtcgtc gttcggcgcc ggcttcagct gtcatggcgc
     6121 actgctgaac gtcgcatgag cacaaaggtc gctaccgccg acgcggcgcc tcgctggccc
     6181 cggcgcgtcc tcccggagct gctcgacgag ctcgcgccga acgacccgcg cgcgcgccgc
     6241 tcgcgacgcg acctgcgcct cgtcaatcga atcatgggcc atccgctgct gctggcgcgg
     6301 gcgctcgacg cagtcgtcac gagggcgccg ctgcgcctcg tcgagctcgg cgccggcgac
     6361 ggc
//
LOCUS       DATLEE010000585         3049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2941073, whole genome shotgun sequence.
ACCESSION   DATLEE010000585 DATLEE010000000
VERSION     DATLEE010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2941073"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1459)
                     /gene="hisS"
                     /locus_tag="VM692_12690"
     CDS             complement(185..1459)
                     /gene="hisS"
                     /locus_tag="VM692_12690"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HUQ53074.1"
                     /translation="MAELIQPVKGMNDVLADQSPWWDRLDAVAAELFASYGYQRIRLP
                     LLERTELFKRSIGELTDIVSKEMFTFEDYGDSLTLRPEGTAGAVRAALSNGLLHNQQQ
                     KLWLAGPMFRREKPQKGRYRQFNQMSVEALGFAEPEIDAELIAMSARLWRELGLTSAK
                     LELNSIGTAESRQVYKQVLVEHFSRHRELLDEDSLRRLDRNPLRILDSKNPALRELID
                     AAPLLSSYLDDESRAHFDRVQALLGDIGVEFTLNPRLVRGLDYYSRTVFEWLTDRLGS
                     QSAICSGGRYDGLVAQLGGRETPAVGWALGIERVVELLQAEGIGAVESAPDVALVAAG
                     DAERRFAFKVAEQLRDRLPGLKLAIGAPAAGFKAQLRRADKSGARFALIVGETERQAG
                     KISLKSLREDQPQQLLTVEEGVDWLRLRSSSA"
     gene            complement(1466..2449)
                     /locus_tag="VM692_12695"
     CDS             complement(1466..2449)
                     /locus_tag="VM692_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HUQ53075.1"
                     /translation="MADSDPPITTAAPPPPPPPQTLGEMLRGARLAHDLTVEQLSTEL
                     RIEARQLNALEENRFEQIGVPVFVKGYLKQYGQRLGLDVRELLALYYKQTTLADVQVQ
                     PSRTIKLHDDRQITTWVLAAVVLLLVAVGLAVWWWNGGSFSALPLTRSAPAPAVSATP
                     SSAEAAAPQRRSVEQPAAPTPTSTPPPAAPTSTPPAAAAALAETPAPAADAAAASDVD
                     SAAESEPAFVAPSDDGDEEPTADVDAIPLELTFDQESWAEVTDARGERLLYGLSAAGR
                     RVAVGGVPPFAIVLGNASVVRLTVDGAPYPIPTTRREGDPSARFSVDVAAD"
     gene            complement(2439..>3049)
                     /gene="pilW"
                     /locus_tag="VM692_12700"
     CDS             complement(2439..>3049)
                     /gene="pilW"
                     /locus_tag="VM692_12700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HUQ53076.1"
                     /translation="AIERLQRALAQNPRLVLAHSTLAVAYDQIGSVEEAETHYLRATQ
                     LAPNDGGAANAYAVFLCRTNRYDDAAPYFRRAADDPNYGTPEVALTNAGVCARDAGKL
                     EAATENFRAALVRNANYGEALRNMMELSYQQRNFLQTRAFVQRYLTAQQPTAPVLWIC
                     FNVERELQDAAAADRCAAQLRSGFRGSNELQQLEEQQRRDGR"
BASE COUNT          481 a         1032 c         1032 g          504 t
ORIGIN      
        1 tccgactgcc tgtcctggaa cgcgaaatac tggcgataac cgtagatagc caacaggccg
       61 attgccacgc cgccgatcaa gaaccagccg ttctcgcgcc accattggcg caagcgctcg
      121 acttgttctt tatctgacaa atagtcgtcc acacgatcct ccggcgggca atgagcgggc
      181 tattctaagc cgagcttgag cgaaggcgga gccagtcgac gccttcttcg accgtcaaaa
      241 gctgctgagg ttggtcctca cgcagcgatt tgaggctgat tttgccggcc tgccgctcgg
      301 tctcgccgac gatcagggcg aagcgggccc cgctcttgtc ggccctgcgc agctgggctt
      361 tgaagccggc ggccggggcg ccgatcgcga gcttgaggcc cggcaggcgg tcgcgaagct
      421 gttcggcgac cttgaacgcg aagcgacgct cggcatcccc ggccgcgacc agcgccacgt
      481 ccggggccga ctctacggcg ccgatgcctt cggcttgcaa cagctcgacg acgcgctcga
      541 tgccgagcgc ccagccgacg gccggcgtct cgcgcccgcc gagctgcgca acgagcccgt
      601 cgtagcgccc gccggagcag atagcgctct gcgagccgag ccgatccgtg agccactcga
      661 atacggtccg cgagtagtag tcgagaccgc ggacgagacg cgggttcagc gtgaactcga
      721 cgccgatgtc gccgagcagt gcctgcacgc ggtcgaagtg cgctcgcgac tcgtcgtcca
      781 agtacgacga cagcagcggc gccgcgtcga tgagctcgcg cagcgccggg ttcttgctat
      841 cgaggatccg cagcggattg cggtcgagcc gccgcaagct gtcctcgtcg agcaactcgc
      901 ggtggcgcga gaagtgctcg acgagcacct gcttgtaaac ctggcgcgac tcggccgtgc
      961 cgatcgagtt gagctcgagc ttcgcgctcg tgaggccgag ctcgcgccag agacgcgccg
     1021 acatcgcgat cagctccgcg tcgatctcgg gctccgcgaa gccgagcgcc tctacgctca
     1081 tctggttgaa ctgccgatag cggcctttct gcggtttctc gcggcggaac ataggcccgg
     1141 cgagccaaag cttctgctgc tggttgtgca gcaagccgtt gctcaaagcg gcgcgcacgg
     1201 ctccggccgt accttcgggc cgcagcgtga ggctgtcgcc gtaatcttcg aacgtaaaca
     1261 tctctttcga gacgatatcg gtcagctcgc cgatcgagcg tttgaaaagc tccgtgcgct
     1321 cgagcagcgg caggcgaatg cgctgataac cgtagctcgc gaacagctcg gccgcgacgg
     1381 cgtcgagccg atcccaccac gggctctgat cggcgagcac gtcgttcatt cccttgacgg
     1441 gttgaatgag ctccgccacg gcgcgtcagt ccgcggcgac gtcgacggag aatcgggcgg
     1501 agggatcccc ttcgcgccgc gtcgtcggga tcgggtaggg cgcgccgtcg accgtcagcc
     1561 gcacgacgct ggcattgccg agcacgatgg cgaacggcgg cacgccgccc acggcgacgc
     1621 ggcgcccggc ggcgctgaga ccgtacagca ggcgctcgcc gcgcgcgtcc gtgacctcgg
     1681 cccagctctc ctgatcgaac gtcagctcga gcggtatcgc gtccacgtcc gcagtcggct
     1741 cctcgtcacc gtcatcggac ggcgctacga acgccggctc ggattccgct gcagagtcga
     1801 cgtcgctcgc ggctgcggcg tcggccgcgg gtgccggcgt ctcggcgagg gcggcagcgg
     1861 ccgccggtgg cgtcgacgtc ggcgcggctg gcggcggcgt cgaggtcggc gtcggcgcgg
     1921 ccggctgctc gacggagcgg cgctgcggcg cggcggcctc agcgctggaa ggcgtcgcgc
     1981 tgacggccgg cgcgggagcg ctgcgcgtca acggcagcgc actgaaacta ccgccgttcc
     2041 accaccaaac cgcaagcccg acggcgacga gcaacagcac gacggccgcg agcacccagg
     2101 tcgtgatctg ccgatcgtcg tgcagcttga tggtgcggct cggctgcact tggacgtcgg
     2161 cgagcgtcgt ctgtttgtag taaagcgcca gcagctcgcg cacgtcgagg ccgagccgct
     2221 ggccgtactg cttcaagtag cctttgacga acaccggcac gccgatctgc tcgaagcggt
     2281 tctcttcgag cgcgttcagc tgccgcgctt cgatccgcag ctccgtcgag agctgctcga
     2341 ccgtcagatc gtgcgcgagc cgggcaccgc gcagcatctc cccaagtgtt tgcggaggcg
     2401 gaggcggcgg cggtgcggcc gtcgtgatcg gtggatcgct atcggccatc gcgcctctgt
     2461 tgctcttcga gctgctgcag ctcgttcgag ccgcggaaac cgctacgcaa ctgcgcggcg
     2521 caccgatcgg cagccgccgc atcttgcagc tcgcgctcga cgttgaagca tatccagagc
     2581 accggcgccg tcggctgctg agctgtaagg taacgctgca cgaacgcgcg cgtttgcaga
     2641 aagttacgct gctggtacga cagctccatc atgttccgca gcgcctcgcc gtagttcgca
     2701 ttgcgaacga gcgcggcgcg gaagttctcc gtggccgcct cgagcttgcc tgcgtcgcgc
     2761 gcacagacgc cggcgttcgt cagcgcgacc tcaggtgtcc catagttcgg atcgtcagcc
     2821 gcgcgacgga agtatggcgc ggcgtcgtcg taacgattcg tccggcagag gaacacggcg
     2881 tacgcgttgg ctgcgccgcc gtcgttgggc gcgagctggg tcgcgcgcag gtaatgagtc
     2941 tcggcctctt cgacactgcc gatctgatcg taagcgaccg cgagcgtcga gtgagccagt
     3001 acgagccgcg gattctgcgc caatgctcgc tgcagccgtt cgatcgcca
//
LOCUS       DATLEE010000586         5007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5914594, whole genome shotgun sequence.
ACCESSION   DATLEE010000586 DATLEE010000000
VERSION     DATLEE010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5914594"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1933
                     /locus_tag="VM692_12705"
     CDS             <1..1933
                     /locus_tag="VM692_12705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HUQ53077.1"
                     /translation="GSDVRLDGASFRVIGVMPQDFGFPQRDIDVYVPFAFTPQQMSDA
                     ARGNQFSTSVGRLRDGATIEGLERELAAIVQRNVAEGRLEGDAVTIAGFMGRAEPLRD
                     LQVGNLRDMLLILQAVVLVVLLIACANLANLQLTRLAGRRKELAVRSALGAAGERIVG
                     MVFVEALLLALAGGALGLAIATGGLELVRALGLDRSNDGFSFGLDATTLAYTLGAAVL
                     AGIASGLPPVLSLLREDLTGAVREAGRQGGGGRRAQRLRSGLVVVQLALSVMLLVGAG
                     LLTKSFYGLLGEGPGFNSGSVWTARVALAGPRYSQQDSWPRFQRQALAELRALPGVGA
                     AGLTSVLPFGGNNSQGTTIIDGYVLPPGGSPPHAQFRSIDEGYLATLGIPIVAGRNFA
                     PHESERVVIIDEILAAKYWPGASPLGQRLRGALDSPDGWYTIVGIVPAVKQGSLAETT
                     IKETVYWHYEQSPSSAAVFALRTVVPPNQLASAVTAAIAAIDPDVAVSDARSLDDRIE
                     SSLGPQRTPMVLTIVFAGAAFVLAVIGIYGVLAWSVTQRVGEIGVRMALGARAADIGR
                     MILSQGGRLIALGLVIGVVGAVALGRVLASQLDRVVSFDLGVLALTVVGLGGAALFAS
                     WLPARRAARVDPLTALRAE"
     gene            1949..4426
                     /locus_tag="VM692_12710"
     CDS             1949..4426
                     /locus_tag="VM692_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ53078.1"
                     /translation="METLRKDIKHAWRLFRENKLFTATAVAALTLGIGVNIAIFSVVN
                     AVLLKPVSFPDPDTLVQVVNANNGVAGGPASSPAKYMHWRAQTDVLDGVAAGRNLNLN
                     LAQGELLESITAGQTTAEYFSVLRPPLVMGRWFTAEEDLPNAGYTVVLSYNFWTQRLN
                     SDPNVLGKSITLSGNPYTVVGVVGREFDVRDLGGAPAEIWVPFQLDPNTTDQGHYFQN
                     VARLKPGVTLEQAQAALMASAAAYRERYTAAAMPETAGFSVITLQESLVRQARPTLFV
                     ALGAVAFVLLIACANVANLLLIRATGRRRELAVRAALGAGRWRIMRQLLTESLMLSFA
                     GGALGLIAGYAGMRWLLTVNTAGLPRLGQGGSLFGMDWRIVAFTVLLSIVTGIVFGLI
                     PALVASRADLNAIIKDSSSRSGSGFRQNKTRSVLVLVEVSLAVVLLVGASLLIRTSLE
                     LGRVDPGYTTENVLIMRTSLTGQQYQRAEVVDETGRLALERIRAIPGVAEGSMTCCVP
                     LQGGLGLPFNIIGRTNEGPFTGGGGIHFAVPGYFDTFKIPVIRGRAFNESDTASAPPV
                     IIINEAMAKQFWKDGADPLADQILVGGGRANMKELTTEPVRQVIGVVGDVRAGSLTQD
                     PGPMMYMPIAQMPDPLNAMLQGMGPMGWVVRTDAEPGSVSQQIQDVIRETTGVPVTNV
                     QNMDDIVSVSTSRQRLNMLLMTIFGGSALVLAAIGIYGLMAYSVQHRTQEIGIRLALG
                     AQADRVRWSIVGQGMTLVGIGVVVGIVTAYFLATFLAAVLYGVQARDVMVFVGVPAVL
                     AATAFGAVWIAARRASGIDPLHALRYE"
     gene            complement(4417..>5007)
                     /locus_tag="VM692_12715"
     CDS             complement(4417..>5007)
                     /locus_tag="VM692_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HUQ53079.1"
                     /translation="EAGIGIWSWASTDDGREPDVVLACAGDVPTLETLAAAELLIELF
                     PDLSLRVVNVVDLMTLQPPSEHPHGLPDRDFDAIFTRDKPIIFAYHGYPWLIHRLTYR
                     RTNHGQLHVRGYKEEGTTTTPFDMVVRNDLDRFHLVGDVAQRVPRLAPRAAYARQLMR
                     DRLAEHREYIREHGEDLPQIRDWTWTGRTEEAAHYS"
BASE COUNT          734 a         1649 c         1753 g          871 t
ORIGIN      
        1 cggcagcgac gtgcggctcg acggtgcgtc gtttcgcgtc atcggtgtga tgccgcagga
       61 tttcggcttt ccgcagcgcg acatcgacgt ctacgtgcca tttgcgttca cgcctcagca
      121 aatgagcgac gccgcgcgcg gcaaccaatt ctcgacgagc gtcggccgac tccgcgacgg
      181 tgcgacgatc gaggggctcg agcgtgagct ggccgcgatc gtgcagcgca acgttgcgga
      241 agggcggctg gagggcgacg ccgtcacgat cgcgggcttc atgggccgcg ctgaaccgct
      301 acgcgacctg caggtcggca acctgcgcga catgctgttg atcctgcaag ccgtcgtgct
      361 cgtcgttttg ctgatcgcct gcgccaatct cgcgaacttg cagctgacga ggttggcggg
      421 gcgccgcaaa gagctcgccg tgcgctcggc gctcggtgcc gcgggtgagc gcatcgtcgg
      481 gatggtgttc gtcgaagcgc tgctgctcgc gctcgcgggc ggcgctctcg ggctcgccat
      541 cgccacgggc ggtctagagc tcgtgcgcgc gctcggtctc gaccgctcca acgacggttt
      601 ctcgttcggg ctcgatgcga ccacgcttgc gtatacgctc ggcgccgccg tgctcgcggg
      661 aatcgcgtcg ggcttgccgc ccgtgttgtc cctgctgcgc gaggacctca cgggcgccgt
      721 gcgcgaagcg gggcggcagg gcggcggcgg gcgccgcgcc cagcgactgc gcagcggtct
      781 cgtcgtcgta cagcttgcgc tcagcgtcat gctgctcgtt ggcgcgggct tgctgacgaa
      841 aagcttctac ggcttgctcg gtgaaggccc cggcttcaac tcgggcagcg tgtggacggc
      901 gcgcgtcgca ctggcggggc cgcggtactc gcaacaggac tcgtggccgc gcttccaacg
      961 ccaagcgctc gcggagctgc gcgcgttgcc gggcgtcggt gctgcgggtc tgacctcggt
     1021 cttgccgttc ggcggcaaca acagtcaagg cacgacgatc atcgacggct atgtgctgcc
     1081 gccgggcggc tcgccgccgc acgcgcagtt tcgctcgatc gatgaaggct acctcgcgac
     1141 gcttgggatt ccgatcgtcg cgggccgcaa tttcgcgccg cacgagtcgg aacgcgtcgt
     1201 catcatcgat gaaatcctgg cagccaagta ttggcccggc gcgagtccgc tcggccagcg
     1261 gttgcgcggg gcgctcgatt cgccggacgg ttggtacacg atcgtcggca tcgtgccggc
     1321 cgtgaagcag ggtagcctcg ccgagacgac gatcaaagag accgtctatt ggcactacga
     1381 gcaaagcccg tcgtcggctg ccgtgttcgc gttgcgcacg gtggtgccgc cgaatcagct
     1441 cgcaagcgca gtgacggcgg caatcgccgc gatcgatccc gacgtggccg tctccgatgc
     1501 ccgatcgctc gacgatcgca tcgaaagctc gctcggccca cagcgaacac cgatggtgtt
     1561 gacgatcgtg ttcgccggcg cggcgttcgt gctggccgtc atcggcatct acggcgtgct
     1621 cgcgtggtcc gtgacgcagc gcgtcggcga gatcggcgtg cgcatggcgc tcggcgcgcg
     1681 tgccgccgac atcggccgca tgatcctgag ccagggcggc cggctcattg cgctcgggct
     1741 cgtcatcggt gtcgtcggcg ccgtggcgct gggccgtgtg ctggcgtcgc agctcgacag
     1801 agtcgtgtcg ttcgatctcg gggtgctcgc gctgaccgtc gtcgggctcg gcggcgcggc
     1861 gctcttcgca agttggctgc cggcgcgtcg cgccgcgcgc gtcgacccgc tgaccgcgtt
     1921 gcgggctgaa taactaagga gcaagtcggt ggaaactctg cgcaaagaca tcaagcacgc
     1981 gtggcggctg ttccgcgaga acaagctgtt cacggcgacg gcagtggcgg cgctgacgct
     2041 cggcatcggc gtgaacatcg cgatcttttc cgtcgtcaac gccgtgctgc tgaagcccgt
     2101 ttcgttcccg gaccccgaca cgctcgtgca ggtcgtcaac gcgaacaacg gcgtcgcggg
     2161 cggccctgcg tcgtcgccgg cgaagtacat gcactggcgc gcccagaccg acgtgctcga
     2221 cggcgtcgcg gcggggcgca acctcaatct gaacctcgcg cagggcgagc tgctcgagtc
     2281 catcacggcg gggcaaacga cggcggagta cttcagcgtg ctgcggccgc cgctcgtcat
     2341 gggccggtgg tttacggccg aggaagactt gcccaacgcg ggctacaccg tcgttctgag
     2401 ctacaacttc tggacgcagc ggttgaacag cgaccccaac gtcctcggca agagcatcac
     2461 gctgtccggc aacccctaca cggtagtcgg cgtcgtcggc cgcgagttcg acgtccgcga
     2521 tctcggtggg gcacccgcgg aaatctgggt gccgttccaa ctcgatccga ataccacgga
     2581 ccaaggtcat tacttccaga acgtcgcgcg cttgaagccg ggtgtcacgc tcgagcaagc
     2641 gcaggcggcg ctgatggcgt cggccgcggc gtaccgcgag cgctacacgg ccgcggcgat
     2701 gccggagacg gccggcttca gcgtgatcac gctgcaggag tcgctcgtgc gccaagcgcg
     2761 cccgacgctg ttcgtcgcgc tcggcgcggt cgcattcgta ctgctgatcg cgtgcgccaa
     2821 cgtcgcgaac ctgctgttga ttcgcgcgac gggacggcgc cgcgagctcg ccgtgcgcgc
     2881 ggcgctcggc gcggggcgct ggcgcatcat gcggcagctc ctgaccgaga gcctgatgct
     2941 gtcgttcgcg ggcggcgcac tcgggctcat cgcgggctat gccggcatgc gctggctgct
     3001 gaccgtcaac acggccggcc tgccgcgact cggccagggc ggctcgttgt tcggcatgga
     3061 ctggcgcatc gtcgcgttca cggtcctgtt gtcgatcgtc acgggcatcg tcttcggact
     3121 gattcccgcg ctcgtcgcgt cgcgcgccga cttgaacgcc atcatcaagg attcgagcag
     3181 ccggtcggga agcggcttcc gccaaaacaa gacgcgctcg gtgctcgtgc tcgtcgaggt
     3241 cagtcttgcc gtcgtgttgc tcgtgggcgc ttcgctgttg attcgcacgt cgctcgagct
     3301 cggcagggtc gatcccggat acacgaccga gaacgtgctg atcatgcgca cgtcgctgac
     3361 ggggcagcag tatcagcgcg ccgaggtcgt cgacgagacg ggccgtttgg cgctcgagcg
     3421 catccgcgcg attcccggcg tcgcagaggg ctcgatgacg tgctgcgtgc cgctgcaagg
     3481 cggtctcgga ttgccgttca acatcatcgg ccgcacgaac gaggggccgt tcacgggcgg
     3541 cggcggcatc cacttcgccg ttccgggcta tttcgacacg ttcaagatcc ccgtcatccg
     3601 cggccgcgcg ttcaatgaga gcgacacggc gtcggcgccc ccggtcatca tcatcaacga
     3661 ggcgatggcg aagcaatttt ggaaggacgg ggccgatccg ctcgcggacc agatcctggt
     3721 cggcggcggc cgagccaaca tgaaggagct cacgaccgag cccgtgcgcc aagtcatcgg
     3781 cgtcgtcggc gacgtgcgtg ccggcagcct cacgcaggat ccggggccga tgatgtacat
     3841 gccgatcgcg cagatgccgg acccgttgaa cgcgatgctg cagggcatgg ggccgatggg
     3901 ctgggtcgtc cgcaccgacg cggagcccgg cagcgtctcg cagcagatcc aagacgtcat
     3961 tcgcgagacg accggcgtgc ccgtgacgaa cgtgcagaac atggacgaca tcgtctcggt
     4021 cagcacctcg cgccagcgtt tgaacatgct gctgatgacg atcttcggcg ggtcggcgct
     4081 cgtgctggcc gcgatcggca tctacggctt gatggcgtac tcggtgcagc atcgcacgca
     4141 agagatcggc attcgcctcg cgctcggcgc gcaggccgac cgcgtgcgct ggagcatcgt
     4201 cgggcagggc atgacgctcg tcggcatcgg cgtcgtcgtc ggcatcgtga ctgcgtactt
     4261 cttggccacg ttcctcgcgg ccgtgctcta cggcgtgcag gcgcgcgacg tcatggtatt
     4321 cgtgggcgtg ccggccgtgc tggccgcgac ggcgttcggc gccgtgtgga tcgcggcgcg
     4381 gcgcgcgagc ggcatcgatc cgctgcacgc gttgcgctac gagtagtgag cagcctcttc
     4441 ggtacgccct gtccaagtcc agtcgcgaat ctgcggcaga tcctcgccgt gctcgcgaat
     4501 gtactcgcga tgctcggcga ggcggtcgcg catgagttgg cgcgcgtagg cggcgcgcgg
     4561 cgcgagccgc ggcacgcgct gcgcgacgtc gccgaccaag tgaaaccggt cgagatcgtt
     4621 gcgcacgacc atgtcgaacg gcgtcgtggt cgtgccttct tctttgtagc cccgcacgtg
     4681 cagctggccg tggttcgtgc gtcgatacgt caagcgatgg atcagccacg gatagccgtg
     4741 atacgcaaaa atgatcggct tatcgcgcgt gaagatcgcg tcgaaatcgc ggtccggcaa
     4801 gccgtgcggg tgctcgctcg ggggctgcag cgtcatcaag tcgacgacgt tgacgacgcg
     4861 cagcgacaag tccggaaaca gctcgatcag cagctccgcc gcggccaacg tctcgagcgt
     4921 cggcacgtcg ccggcgcatg cgaggacgac gtcgggctcg cggccgtcat ccgtgctcgc
     4981 ccatgaccag atgccgatgc cggcttc
//
LOCUS       DATLEE010000587         2255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2196211, whole genome shotgun sequence.
ACCESSION   DATLEE010000587 DATLEE010000000
VERSION     DATLEE010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196211"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VM692_12720"
     CDS             <1..224
                     /locus_tag="VM692_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HUQ53080.1"
                     /translation="GQRAVHALVWLERESHKSIVIGKQGQVLKQAGTEARQELRHLLG
                     ERVHLELWVKVREHWADSERELQRLGFEV"
     gene            224..952
                     /gene="recO"
                     /locus_tag="VM692_12725"
     CDS             224..952
                     /gene="recO"
                     /locus_tag="VM692_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HUQ53081.1"
                     /translation="MAEREQVLLERGYVLHQRPYRDSSQLLECMTATRGRVGLVARGS
                     RSSPTRQRALLQPFVPLKLSWVRRGDLGRLTHVEAEGLSHDLTGQRLLAGYYANELLL
                     RLLARGDPNGEVFSCYSRCLAQLGETASVARTLRVFELELLRALGYGLELDGDSATGE
                     PLREQLRYAYELEQGLRRAEPRDADADLYSGRDLIALRDLTLDDDASLRTAQRLLGRV
                     LREHLGERPLQSRLVLQDIVSRGL"
     gene            963..1691
                     /gene="pdxJ"
                     /locus_tag="VM692_12730"
     CDS             963..1691
                     /gene="pdxJ"
                     /locus_tag="VM692_12730"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HUQ53082.1"
                     /translation="MGLIHLGVNVDHVATLRQARHTRYPDPVAAALIAEQSGADSITV
                     HLREDRRHIQARDVEVLLRTVQTRVNLEMAVTESMLDFAVKHRPSDCCFVPERREELT
                     TEGGLDVAGQKQRVAAACRTLQQHGVRVSLFIDPEIAQVEASAEAGAPVVELHTGAYA
                     EAGGAAAAHELARIETAAQRAQALGLTVHAGHGLNYHNVTAVAARPEIVELNIGHAIV
                     AQAVFDGIAEAVAKMKALMVAARA"
     gene            1688..2083
                     /gene="acpS"
                     /locus_tag="VM692_12735"
     CDS             1688..2083
                     /gene="acpS"
                     /locus_tag="VM692_12735"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HUQ53083.1"
                     /translation="MIFGIGTDVLQVERVDKTWQRFGEHFARRLLLDEERELFAKAKR
                     PVRFLAMRFAAKEAIVKALGTGFANGIWVRDIGMVPNKLGQPQVIYSERGRALCRKLG
                     VGEGHLTLSDEAGLIVAVAVLMRAAPEKS"
BASE COUNT          384 a          715 c          786 g          370 t
ORIGIN      
        1 aagggcagcg tgccgtccac gcattggtgt ggctcgagcg agagagccac aagagcatcg
       61 tcatcggcaa gcagggtcag gttctaaagc aggccggcac cgaggcgcgc caagagctgc
      121 gtcatttgct cggcgagcgc gtgcacctcg agctctgggt caaggttcgc gagcactggg
      181 ccgacagcga acgcgagctg cagcggctcg ggttcgaggt gtaatggccg agcgcgaaca
      241 ggtattgctc gagcgcggct acgtgctgca ccagcgaccc tatcgcgact ccagtcagtt
      301 gctcgaatgc atgacggcga cgcgcggccg cgtgggactc gtggcgcgcg gttcgcgttc
      361 gagcccgacg cgccagcgag cgttgctgca accgttcgtg ccgctgaagc tgtcgtgggt
      421 cagacgcggc gatctcggac gcttgacaca tgtcgaggca gaggggctga gccacgatct
      481 gacgggtcag cgtctgctcg cgggttacta cgcgaacgaa ttattgctgc gattgctcgc
      541 gcggggcgat ccgaacggcg aagttttttc ctgctatagt cgctgcctcg cgcagcttgg
      601 cgaaacggcg agcgtggcgc gcaccctgcg cgttttcgag ctcgagctgc tgcgtgcgct
      661 cggttacggg ctcgagctcg acggcgattc ggcgaccggc gagccgttgc gcgagcagct
      721 gcgctacgcc tacgagctcg agcaagggct gcgacgcgcg gagccacggg atgccgacgc
      781 ggatctgtac tcgggccgag acttgattgc gctgcgggac cttacgctcg acgacgacgc
      841 cagtcttcgc acggcacagc ggctgctcgg gcgtgtgctg cgcgagcatc tcggcgagcg
      901 gccgctgcaa agccggctcg tgctgcagga catcgtgagc cgcggattgt agtgacacgc
      961 acatgggact cattcatctc ggcgtcaatg tcgatcacgt tgcaaccctg cggcaagccc
     1021 gccacacgcg ttatcccgac cccgtcgcgg ccgcgctgat tgccgagcag tccggcgccg
     1081 acagcatcac cgtgcatctg cgcgaggacc gtcgccatat tcaggcgcgc gacgtcgaag
     1141 tcttgctgcg caccgtgcag acccgcgtga atctcgagat ggccgtcacg gagtcgatgc
     1201 tcgacttcgc cgtcaagcac cggccgtccg actgctgctt cgtgccggaa cggcgcgaag
     1261 agctgacgac cgaaggtggc ctcgacgttg ccgggcagaa gcaacgcgtt gcggcggctt
     1321 gccgcacgct ccagcaacac ggtgtgcgcg tgtcgttgtt catcgatccc gagattgcgc
     1381 aagtcgaagc gagcgccgaa gcgggcgctc cggtcgtcga gctgcacacg ggtgcctatg
     1441 ccgaggccgg cggtgcagcc gcggcacacg agcttgcgcg catcgaaacg gcggcgcagc
     1501 gcgcgcaagc gctcgggctc acggtgcatg ccgggcacgg gttgaactat cacaacgtga
     1561 cggccgtcgc ggctagaccc gagatcgtgg agctcaacat cggtcacgcg atcgtcgcgc
     1621 aagccgtgtt cgacggcatc gccgaagccg tcgccaagat gaaagcgctc atggtcgctg
     1681 cacgagcgtg atcttcggta tcggcacgga cgtcctgcag gtggagcgcg tcgacaagac
     1741 gtggcagcgc ttcggcgagc acttcgcacg ccgtctgctg ctcgacgagg agcgcgagct
     1801 gttcgcgaaa gcgaagcggc ccgtgcggtt tctcgcgatg cggttcgccg ccaaggaagc
     1861 aatcgtcaaa gcgctcggca ccgggttcgc gaacggcatt tgggtgcgcg acatcggcat
     1921 ggtgccgaac aagctcggcc agccgcaggt catctactcg gagcgcggcc gcgcgctgtg
     1981 ccgcaagctg ggggtcggcg agggacattt gacgctcagc gacgaggccg ggctcatcgt
     2041 ggcggtcgcc gtattgatgc gagcggcgcc ggagaaatcg tgaacacgct ggtattcgat
     2101 atcgagaccg tgcccgacgt cgagctcggc cggcggctct acggcctgca ggacctcgac
     2161 gacgcggccg tcgcgcaagc gatgttcgcg aagcaacgcg ggctgcgtgc gaacgacttc
     2221 ctgccgccgc cgcagcagcg catcgtcgca atctc
//
LOCUS       DATLEE010000588         2506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3349886, whole genome shotgun sequence.
ACCESSION   DATLEE010000588 DATLEE010000000
VERSION     DATLEE010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3349886"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VM692_12740"
     CDS             <1..318
                     /locus_tag="VM692_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53084.1"
                     /translation="GQSNGRWDGDSLVVETTGFNGMTHLDRAGNHHSSALKVTERFTP
                     VGENHLQYSATLDDANTYSRPWTISMPLYRHIEPNAQILEFKCVPFVEELLYKDLELP
                     QPQ"
     gene            431..847
                     /locus_tag="VM692_12745"
     CDS             431..847
                     /locus_tag="VM692_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53085.1"
                     /translation="MRSKLNGLAASVGLSLTAAVPAVAHHSFAAEFDANKPVTLEGTV
                     KEFRWVNPHSWLHINVTKPDGTIEEWAVEGGAPSALLRRGWTRDTLPPGTKVTVQGFM
                     SKDGALRANARDITFPDGKQLFIGSSGIGAPDERKE"
     gene            complement(927..1610)
                     /locus_tag="VM692_12750"
     CDS             complement(927..1610)
                     /locus_tag="VM692_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HUQ53086.1"
                     /translation="MIDPRQLLEQVLGGNLPGAGRSLQSQLDRQSGSRGFAGGAMAGG
                     LLGMLLGGGRRRGFGGGGIGTAVLGALAMQAYSTYQQRQQGLRSAPPPPANFDSQALP
                     HARPAADGSPFELTLVRAMVGAAKADGHIDAAEQRRLFAEVERLGLDSESKAYVFDLL
                     TGDIDVASLVAAVDTPEQGAELYLAARLAIDPDEPTERAYLDTLAERLRLPAELRVTL
                     DAAPQKATA"
     gene            complement(1612..>2506)
                     /locus_tag="VM692_12755"
     CDS             complement(1612..>2506)
                     /locus_tag="VM692_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008030464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HUQ53087.1"
                     /translation="PLRALLALAPRAKGGRRLAPAADTPRAARVEPARKRVALVTGCV
                     QTVIAPEINAATIRVLERCGVEVVTVDGCCGAIVHHLGKERGANALAAALVERLSAEL
                     DGAGLDAIVANASGCGTHVKDYGYVLRHDAELAAKAAAVSAKARDVTELLAEIGLPAV
                     APSAPKLTVAYHSACSMQHGQKVEREPRALLTAAGFEVREIAEGHLCCGSAGTYNILQ
                     PELATRLRSRKLGNIARTGAEVVATGNIGCMTQLAAAARVPVVHTVELLDFATGGPLP
                     PALANLPNTAASNAARANGGN"
BASE COUNT          428 a          866 c          831 g          381 t
ORIGIN      
        1 ggccagtcga acgggcgctg ggacggcgac tcgctcgtcg tcgagacgac gggcttcaac
       61 ggcatgacgc acctcgatcg cgccggcaat caccactcga gcgcgctgaa ggttacggaa
      121 cgcttcacac cggtcggcga gaaccatttg caatactcgg ccacgctcga cgacgcgaac
      181 acctactccc ggccgtggac gatcagcatg ccgctgtacc ggcatatcga gccgaacgcg
      241 cagatcctcg aattcaagtg cgtgccgttc gtcgaagagc tgttgtacaa agacctcgag
      301 ttgccgcagc cgcaataggc ggaattccca gaaatcccca agcggttgta ccagcggcgg
      361 aaggccccga gatcgagata aactagacac ctgagctcgg tacaacccga gagcctgtag
      421 gagagtagcg atgcgtagca aactcaatgg cctggccgcg agcgtcggcc tgtcgctgac
      481 ggcagcggta ccggcggtcg cgcatcactc cttcgccgcg gaattcgacg ccaacaagcc
      541 cgtcacgctc gagggcaccg tcaaggaatt ccgctgggta aatccgcact cgtggctgca
      601 catcaatgtc acgaagcccg atgggacgat cgaagagtgg gccgtcgaag gcggcgcgcc
      661 gagcgcgctg ctgcgccgcg gctggactcg tgacacgttg ccgcccggca cgaaggtcac
      721 ggtgcaaggg ttcatgtcga aggacggtgc gctgcgcgcg aacgcgcgcg atatcacgtt
      781 ccccgacggc aagcagttgt tcatcggctc gtcgggcatc ggcgcgccgg atgagcgcaa
      841 ggaatagccc gccgcgctaa cgccgttcga gcgtgccggt gttacggttt cggctcaacc
      901 gaaccgtaac accggcacct tcgtttttag gcggtggctt tctgcggcgc ggcgtcgaga
      961 gtcacgcgca gctccgccgg caagcgcaaa cgctcggcga gcgtgtccag ataggcgcgc
     1021 tcggtcggct cgtcgggatc gattgcgagc cgcgccgcta agtacagctc ggcgccctgc
     1081 tccggcgtgt cgacggccgc gacgagactc gcaacatcga tgtcgcctgt cagcaggtcg
     1141 aagacgtacg ctttcgattc ggagtccaag ccgagccgtt cgacctctgc gaacaaacgg
     1201 cgctgctccg ccgcgtcgat gtgaccgtcc gccttcgcgg cgccgaccat cgcacgaacg
     1261 agcgtgagct cgaatggact gccgtcagcg gccggccgcg cgtgcggcag cgcttgcgaa
     1321 tcgaaattcg ccggcggcgg cggagcgctg cgcagaccct gctgccgctg ctgatacgtg
     1381 ctgtacgcct gcatcgccag cgcacccagc acggccgtgc cgatgccgcc gccgccgaac
     1441 ccgcgccgtc ggccgccgcc gagcagcatg ccgagtagcc cgcccgccat ggcgccgccc
     1501 gcgaacccgc gcgagccgct ctgccgatcg agctgcgatt gaagactgcg gcccgcgccg
     1561 ggcaaattgc ccccgagaac ctgctcgaga agttgcctcg gatcgatcac gtcagttacc
     1621 tccgttagcc cgtgcagcat tcgatgccgc ggtgtttggt aagttcgcga gcgccggcgg
     1681 caacggcccg ccggtcgcaa agtccagcag ctcgaccgtg tggactaccg gcactcgagc
     1741 cgcggccgcg agctgcgtca tgcagccgat gttaccggta gccacgacct cggcgcccgt
     1801 gcgcgcaatg ttcccaagct tacgcgagcg cagccgtgtc gcgagctcgg gctgcaaaat
     1861 gttgtacgtg ccggccgagc cgcagcagag atgcccttcg gcgatctccc ggacttcgaa
     1921 accggccgcc gtcagcagcg cgcgtggctc ccgctccacc ttctggccgt gctgcataga
     1981 gcatgccgag tgataggcaa cggtcagttt gggcgcgctc ggcgcgacgg ccggcaaccc
     2041 gatctcggcc aagagctccg tcacgtcgcg cgccttcgcg ctcacggcgg ccgctttggc
     2101 cgcgagctcg gcatcgtggc gaagcacgta accgtagtcc ttgacgtgcg tgccgcagcc
     2161 ggaggcattg gccacgatcg catcgaggcc cgcgccgtcg agctcggcgc tgagccgctc
     2221 gacgagagcc gccgcaagcg cgttggcgcc gcgctccttg ccgagatggt gcacgatcgc
     2281 gccgcagcag ccgtcgaccg taacgacctc gacgccgcag cgctcgagca cgcggatcgt
     2341 cgcggcgttg atctcgggag cgatgacggt ctgcacgcag cccgtcacga gcgcgacgcg
     2401 cttgcgcgca ggttcgacgc gcgccgcgcg cggtgtgtcg gcggcgggag caaggcggcg
     2461 cccccctttg gctcgcggtg ccagcgcgag cagcgcgcgc aacggc
//
LOCUS       DATLEE010000589         3653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_712902, whole genome shotgun sequence.
ACCESSION   DATLEE010000589 DATLEE010000000
VERSION     DATLEE010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712902"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /gene="gshA"
                     /locus_tag="VM692_12760"
     CDS             <1..1623
                     /gene="gshA"
                     /locus_tag="VM692_12760"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HUQ53088.1"
                     /translation="GEHFRVLCCVSVSPSSRSPASGGPFAARLEWLRDGGRADLIRHG
                     LRGVEKESLRVSLDGTLSRRPHPRAFGAALTHPYITTDYSEALPELVTPPQRSQWETL
                     QFLCDVHAFIGRRLDGELLWPASMPCELPPDDEIPIAFYGPSNLGRMKTVYRRGLGYR
                     YGRAMQAIAGVHFNYSLPLKFWPEYQVRSGHAGSLQEFRSTELMGLVRNYRRAAWLVT
                     FLFGASPALNRSFRPDGHELLTELDAATWFAPYATSLRMSDLGYQNKTQARLSIRANS
                     LAEYMAGMRSAVTTEEPRYAAIGVVVDGDYRQLNANILQIENEYYSTIRPKPSKANKH
                     RPLEALRETGIDYVEVRTLDLNSADPVGMNQSELRFIEALLIYCLLSESPPISAAEQD
                     EIDARSLTVAREGRRPALTFVDGGRERRLRDRGLEMLEGIRAVAELLDSDAQGYVAAV
                     DAAADALRDPERTPSALLLAALKSEQASFFEYTLGLARSHAAYFRDFALAEEREELLL
                     ETARRSHEEADALVAKDPRSFEAYLRDYFAEA"
     gene            1724..2389
                     /locus_tag="VM692_12765"
     CDS             1724..2389
                     /locus_tag="VM692_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53089.1"
                     /translation="MKTLFLAAIVCGFAGTLAAAHYVPFLQHERLPSHTSVVANGGRS
                     EQFLIRLPADRIAATDSEAGGLRSTGVDGAMLLPAQFVGEPLLVEHFKIRDSADTVVG
                     LAARHWHGSGAAATTTWSILIPSRGSLVLNAFGEPRGALESALRTNGYGAGKAWDGKV
                     TVDMIPQGQRGSVATGTGEFAALSGSYTETWTVAAIDENGRLSGTVELNTITSLPESS
                     VPE"
     gene            2386..3144
                     /locus_tag="VM692_12770"
     CDS             2386..3144
                     /locus_tag="VM692_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53090.1"
                     /translation="MKYLLVAIVGLVLGAAAAGVVLYFNPLADKSAAQPNATDRVLHY
                     SLPDQVLEFAVGEDARMFGQDAGHDSLWEGTINRTAVLGLVLNDAANQPAAIASRLIA
                     PSADTDLLLRGVLLDDLWLVTLPGEGTLFVSAESNAWPFLKKTLVPVWYLARPWKGPA
                     DYWPTVGPGTDNSGVVLGLTGALRGSEGSVVEHYEIAALDRERDVALAKAELHLNMPG
                     PRVAAQPPQVAAQPPRVATQPPGVATQPAKGAAQ"
     gene            complement(3148..>3653)
                     /locus_tag="VM692_12775"
     CDS             complement(3148..>3653)
                     /locus_tag="VM692_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HUQ53091.1"
                     /translation="GIVPNVELAAAAGLPCSNGIVVDEFARTSDPHIFAAGDCTNHPL
                     PLLGRRVRLESVPNAIHQAKVAAASLLEAPVAYSEVPWFWSDQYDLKLQIAGLSTGFD
                     EVVLRGDPATRSFAAFYLAAGQVLAVDAINSPKEFIAGKKLVANRARIAPDVLRDPGV
                     DLTPLAG"
BASE COUNT          585 a         1205 c         1232 g          631 t
ORIGIN      
        1 ggtgagcatt ttcgagttct ctgctgcgtg agcgtaagcc cgtcatctcg atctcctgct
       61 tccggcgggc cctttgcggc gcgcctcgaa tggctgcgtg acggcggccg cgcggacctg
      121 atccggcatg gcttgcgcgg cgtagagaaa gagagcctgc gcgtgagtct cgacggcacg
      181 ttgtcgcggc ggccgcatcc gcgcgccttc ggcgctgcac tgacgcatcc gtacatcacg
      241 accgattatt ccgaagcttt gcctgagctc gtcactccgc cgcagcgctc gcagtgggag
      301 acgctgcagt tcctgtgcga cgtgcacgcg ttcatcggtc ggcgtctcga cggcgagctg
      361 ctctggccgg ccagcatgcc gtgcgagctg ccgccggacg acgagatacc gatcgcgttc
      421 tacggcccgt cgaatctcgg ccgcatgaaa accgtctatc gccgcgggct cggctatcgc
      481 tacggtcgcg cgatgcaagc aatcgcgggc gtgcacttca actattcgtt gccgctgaag
      541 ttttggccgg agtatcaggt gcgctcgggt cacgcgggat cgctgcagga gttccgctcc
      601 accgagctca tggggctcgt ccgcaattac cgtcgcgcgg cttggctcgt gacgttcctg
      661 ttcggagcgt cgcctgcgct caatcgctcg ttccggccgg acggtcacga gctcttgacc
      721 gagctcgacg ccgcgacttg gttcgcgccg tacgcgacgt cgctgcgcat gagcgatctc
      781 ggctatcaaa acaagacgca agcccgattg agcatccgcg cgaattcgtt ggccgagtac
      841 atggcgggga tgcgcagcgc ggtgacgacc gaggaaccgc ggtacgcggc gatcggtgtc
      901 gtcgtcgacg gcgactaccg gcagctcaac gcgaacattt tgcagatcga gaacgagtac
      961 tacagcacca ttcgcccgaa gccgagcaag gccaacaagc atcggccgct cgaggcgctg
     1021 cgcgaaaccg gcatcgacta tgtcgaagtg cgcacgctcg atctgaacag cgccgatccc
     1081 gtcggcatga accagagcga gctgcgcttc atcgaagcat tgctgattta ttgtttgctc
     1141 agcgagagcc cgccgatctc ggctgcggag caggacgaaa tcgacgcgcg ctcgttgacg
     1201 gtggcgcgcg aaggccgtcg gccggcactg acgttcgtgg acggcggccg cgagcgacgg
     1261 ctccgcgatc gcggcctcga gatgctcgaa ggtatccgcg ccgtggccga gctgctcgac
     1321 tccgatgcgc agggttacgt cgcggccgtc gacgccgccg cagacgcgct gcgcgacccc
     1381 gagcgcacgc cgtcggccct cctgctcgcg gcactgaaga gtgagcaagc gagctttttc
     1441 gagtacacgt tggggctcgc ccgcagccac gcggcgtatt tccgcgattt cgccttggcc
     1501 gaggagcgcg aggagctgct gctcgagacc gcccgtcgct cgcacgagga ggccgacgcg
     1561 ctcgtggcca aggacccgcg gtcgttcgag gcttatctgc gggactattt cgcggaggct
     1621 tgagccgggc cccgtcgacg caggtcgttt tttgtgattc cgttaaccgc cgcgcttggg
     1681 ccaacgcgcg gatactgcga cccttgatct atccgggacg ggcatgaaga cactgtttct
     1741 ggcggcgatc gtttgcggct tcgcgggcac gctcgccgcg gcgcactacg ttccgttctt
     1801 gcagcacgaa cgcttgccgt cgcacacgtc cgtcgtcgcc aatggcggcc gttcggagca
     1861 gttcttgatt cgcttgcccg ccgaccggat cgccgccacg gactcggaag ccggcggtct
     1921 gcgaagcacc ggcgtcgacg gcgcgatgtt gttgcctgct cagttcgtcg gggagcccct
     1981 gctcgtcgag catttcaaga ttcgcgactc cgcggatacc gtcgtcggtc ttgcggcgcg
     2041 tcattggcac ggcagcggcg cggcggcgac gacgacgtgg tcgatcctga taccaagccg
     2101 cggctcgctg gtgctgaacg cgttcggcga gccacgcggc gcgctcgagt ctgcgctgcg
     2161 cacgaacggc tacggcgccg gcaaagcgtg ggacggcaaa gtcaccgtgg acatgattcc
     2221 gcagggccag cgcggcagcg tggccacggg caccggcgag ttcgcggcac tgtccggcag
     2281 ctacaccgaa acctggaccg tcgcggccat cgacgagaac ggccgcttga gcggtaccgt
     2341 cgagctcaac acgatcacga gcttgcccga atcgagcgtg cccgaatgaa gtatctactc
     2401 gtcgcgatcg tcggtctcgt gctcggtgcg gccgcggcgg gcgtagtgct gtacttcaat
     2461 ccgctggccg acaagtcggc ggcgcagccg aatgccacgg atcgcgtgct gcattactcg
     2521 ttgcccgatc aagtgctcga gttcgccgtc ggcgaggatg cgcgcatgtt cggacaagac
     2581 gccggtcatg acagcctgtg ggaaggtacg atcaaccgca ccgcagtgct cgggctcgtg
     2641 ttgaacgacg cagcgaatca gcccgccgcg atcgcgagcc gtctcatcgc gccttcggcc
     2701 gacacggact tgctgctgcg cggcgtgctt ctcgacgatc tctggctcgt tacgctgccc
     2761 ggcgaaggga cgctgttcgt cagcgccgag tcgaacgctt ggccgttttt gaagaagacg
     2821 ctggttcccg tctggtatct ggctcggccg tggaagggcc cagcggacta ttggccgacc
     2881 gtcggtcccg ggacggacaa cagcggcgtc gtgctcggcc tgacgggcgc gttgcgcggc
     2941 agtgaaggca gcgtcgtcga gcactacgag atcgcggctc tcgatcgcga gcgcgacgtc
     3001 gcgctcgcaa aggccgagct gcacctgaac atgccagggc cgcgcgtcgc ggcacaaccg
     3061 ccgcaagtcg cggcacaacc gccgcgcgtc gcgacccaac cgccgggcgt cgcgacccaa
     3121 cccgccaaag gcgccgcgca gtaactctca gcccgcgagc ggcgtcaggt cgacgccggg
     3181 atcgcgcagc acgtcgggcg cgatgcgcgc gcggttcgcc acgagcttct tgccggcgat
     3241 gaactctttc ggactgttga tcgcgtcgac ggcgagcact tggcccgccg cgagataaaa
     3301 cgcggcgaag ctgcgagtcg cagggtcgcc gcgtagcacg acctcgtcga agcccgtcga
     3361 cagccccgcg atctgtagct tgaggtcgta ctggtccgac cagaaccaag gcacctccga
     3421 gtaggccacg ggcgcctcga gcaagctggc tgcggccact ttggcttggt gtatggcatt
     3481 cggtaccgac tcgagccgca cgcgccgccc gagcaacggt agcgggtggt tcgtgcagtc
     3541 gccggcggcg aagatgtgcg gatccgacgt gcgcgcgaac tcgtcgacga cgatgccgtt
     3601 gctgcacggc aagccggccg cggcggcgag ctcgacgttc ggcacgatgc cga
//
LOCUS       DATLEE010000590         2609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4410002, whole genome shotgun sequence.
ACCESSION   DATLEE010000590 DATLEE010000000
VERSION     DATLEE010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4410002"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VM692_12780"
     CDS             <1..448
                     /locus_tag="VM692_12780"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HUQ53092.1"
                     /translation="GRAGRADRPGEVVIQTHYPDHPLLRCLLEDDYAAFATLALVERR
                     EAHWPPFTHLAVWHAEATRREAAVAFLEQVRDAAAAEQERRVAVLGPAALPMERKDGR
                     YRAQLLFRCADRAPLHRLVERTLLAVRNSPAARRARWSIDIDPLEV"
     gene            512..1108
                     /locus_tag="VM692_12785"
     CDS             512..1108
                     /locus_tag="VM692_12785"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HUQ53093.1"
                     /translation="MARRNHNSRRSDAAPGWVWMLFGLGLGLIVAIGVYLRTPTATRS
                     PEQRTVAVAQPATPKPTSTTQRRSEAAEPRTTAAENKENRFDFYEILPQFEVVVPEDD
                     APPATTTASRPRPAAPPAPGSFLLQAGSFGAAADADRLKASLALLGFESHVQRVTIEN
                     DTFNRVRIGPIADLDTAKRIQRQLRAAGIDPLLMKVPN"
     gene            complement(1109..2446)
                     /locus_tag="VM692_12790"
     CDS             complement(1109..2446)
                     /locus_tag="VM692_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUQ53094.1"
                     /translation="MSARILVVDDEANIRALIDEILSEEGYDVTTAADAKEARGARKR
                     SEFDLILLDIWMPDTDGISLLREWSEGGATGTVVMMSGHGTVDTAVEATRLGAVDFIE
                     KPVSLAKLLRTVERALAVRRSKEQRRGQTTQNSVPAGKSQPMRALREQIARVAQHSAH
                     TLFTGEPGSGRELFARFLASQSAQARAAFVAVMGGDLNDDNAQRELLGDGTEPGAFER
                     AEGGILFIQELGELGSQAQRILLSALEQGTYRIPGQTTDQPFNSRILSSAFQGFERSD
                     TLRPELLSHLSVVVIRVPPLREYPEDVPELLRHHVDLLVDTESLPFRRFSVAAQNRLR
                     NYPWPRNVRELRNMVKRLLILGGPEEISLTEVEAQLATDTADREPLVKQDLLAMPLRE
                     AREHFERAYLQQQLLLCGGKVGQLAKRVGMERTHLYRKLRSLGVDFRQSLTDD"
     gene            complement(2506..2609)
                     /locus_tag="VM692_12795"
                     /pseudo
     CDS             complement(2506..2609)
                     /locus_tag="VM692_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          422 a          903 c          839 g          445 t
ORIGIN      
        1 gggccgcgcg ggccgggccg atcgccccgg cgaggtcgtc attcaaacgc actaccccga
       61 ccatccgctg ttgcgctgcc tgctcgagga cgactacgcg gctttcgcga cgctcgccct
      121 cgtcgagcgg cgcgaagcgc attggccgcc gttcacgcat ctcgcggtgt ggcacgccga
      181 agcgactcgc cgcgaggcgg ccgtcgcatt cctcgagcaa gttcgcgacg cggcggccgc
      241 cgagcaagaa cgacgcgtgg ccgtgctcgg gcccgcggcg ttgccgatgg agcgcaagga
      301 cgggcgctat cgcgcgcagt tgttgtttcg ctgtgcggac cgcgcaccgc tgcatcggct
      361 cgtcgaacgc acgctgctcg cagtgcgcaa ctcgccagcg gcgcggcgcg cgcgctggag
      421 catcgacatc gatccgctcg aggtctaacg cgcccgacgc ggcaacgagc actcggttag
      481 aattgccgcg gtttcacgct cggtctcgaa gatggcccgc cgtaatcaca actctcgccg
      541 cagcgacgcc gcgcccggtt gggtgtggat gttgtttggc ttgggtcttg gcctcatcgt
      601 cgcgatcggc gtctatctgc gcaccccgac cgcgacgcgc tcgcccgagc agcgcaccgt
      661 cgccgtcgcg cagccggcga cgcccaaacc gacgtcgaca acgcagcggc gcagcgaagc
      721 agccgagccg cggacgacag ccgccgagaa caaagaaaat cgtttcgatt tctacgagat
      781 cttgccgcag ttcgaggtcg tggtccccga ggacgacgcg ccgcccgcca ccacaacggc
      841 gtcacggccg cgtccggccg cgcctcccgc gccgggcagc ttcctgctgc aagccgggtc
      901 gttcggtgcc gcggccgacg ccgaccggct caaagcgagc ctcgcgctgc tcgggttcga
      961 atcgcacgtg cagcgagtga cgatcgagaa cgacaccttc aaccgcgtgc gcatcggccc
     1021 catcgccgat ctcgacacgg ctaagcgcat ccagcggcag ctgcgcgccg ccggcatcga
     1081 tcctctgctg atgaaggttc cgaactaact agtcgtcggt cagcgactgg cgaaagtcca
     1141 cgcccaacga gcgaagcttg cggtacagat gcgtgcgctc catgccgacg cgcttcgcga
     1201 gctggccgac tttgccgccg cacaacagca gttgctgctg aaggtatgcg cgctcgaagt
     1261 gctcgcgcgc ttcgcgtagc ggcatcgcga gcaagtcctg cttgacgagc ggctcgcgat
     1321 cggccgtatc ggtcgcaagc tgcgcctcga cctccgtcag cgagatttcc tcgggcccgc
     1381 cgaggatcag caaccgcttg accatgttgc gcagctcgcg gacgttgcgc ggccatggat
     1441 agttgcgcag ccgattctgc gccgcgacgc tgaagcgccg gaacggcaaa ctttcggtgt
     1501 cgacgagcaa atcgacgtga tgacgcagca actccggcac gtcctccggg tactcccgca
     1561 acggcggaac gcggatgacg acgacgctca agtgcgagag caattcgggc cgaagcgtat
     1621 cgctgcgctc gaacccttgg aacgcgctac tcaaaatccg gctgttgaac ggttgatcgg
     1681 tggtttgtcc cgggatgcga taggtgcctt gctcgagcgc gctcaggaga attcgttgcg
     1741 cttggctgcc gagctcgccg agctcctgga tgaagagaat accgccctcg gcgcgctcga
     1801 atgcgccggg ctccgtgccg tcgccgagca gctcgcgctg cgcgttgtcg tcgttcagat
     1861 cgccgcccat gaccgcaacg aacgcggcgc gcgcctgagc gctctggctc gccaggaaac
     1921 gcgcgaacag ctcgcgcccg ctgccgggct cgccggtaaa taacgtgtga gcgctgtgct
     1981 gcgcgacgcg ggcgatctgc tcgcgcagcg cgcgcatcgg ctggctcttg cccgccggaa
     2041 ccgagttctg cgtcgtctgc ccgcggcgtt gctctttgct gcgccgcacc gcgagcgccc
     2101 gctcgacggt gcgtaacagc ttcgcgagcg agacgggttt ctcgatgaaa tcgactgcgc
     2161 cgaggcgcgt cgcctcgacg gccgtgtcga cggtaccgtg acccgacatc atcacgaccg
     2221 tgccggtggc accgccctcg gaccactcgc gcagcagcga gatgccatcc gtgtcaggca
     2281 tccaaatgtc gagcaggatg agatcgaact ctgagcgctt gcgggctccg cgcgcttcct
     2341 tggcgtcggc tgccgtggtg acgtcgtaac cttcttcgct caggatctca tcgatcaaag
     2401 cccgaatatt cgcttcgtcg tcgacaacca gaattcgcgc actcatgcgc gttctctcct
     2461 gtggtcgtgg cgccgagtga gcgactggcc gcccacgtcg acggatattg gaatgagtat
     2521 actgatctcc gccccaccct gctcgcggtt gcgcgcgttg atctgaccgc cgtgctcctc
     2581 gatgagcttc ttcacgatcg cgagaccga
//
LOCUS       DATLEE010000591         3963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5918678, whole genome shotgun sequence.
ACCESSION   DATLEE010000591 DATLEE010000000
VERSION     DATLEE010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5918678"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..807
                     /locus_tag="VM692_12800"
     CDS             166..807
                     /locus_tag="VM692_12800"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HUQ53095.1"
                     /translation="MRRAILVVCLTLLGSGVAHAQQAGQRYVVDQAQSDFHWLVYKAG
                     SLSRLGHNHTIATGDLTGSVTVNEQDRNASQFELSFNVANLVVDDPMLRATLGADFES
                     VPTADDIAGTRKNMLTDKVLDGEKYPSIRILGTGPVGSAGKQELKVKVELLGRTVDLT
                     VPTEVTIAGNEVRAKGEFELTHADLGMKPFSVMLGALQVGENLSFTYDIRARR"
     gene            1021..1905
                     /gene="motA"
                     /locus_tag="VM692_12805"
     CDS             1021..1905
                     /gene="motA"
                     /locus_tag="VM692_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HUQ53096.1"
                     /translation="MLVFVGYAVVVGSVFGGFALAGGHLGAMYQPLELLMIGGAAVGA
                     FVVGNSFKTIKATFKAVPSLFQGSKYTKATYMELMALLYEVLDKTRKEGLLAIERDIE
                     SPKDSALFKKYPRVLKDHHMVEFITDYLRLMVSGKLNAIEIEALMDREIDTHHHEGAE
                     PAHALQKIADGLPAFGIVAAVMGVVHTMESVGLPPQELGILIAHALVGTFLGILLSYG
                     FVAPLVGVLEKKGHEASKALECIKVTLLASLNDAVPTVAVEFGRKVLYSTERPGFSEL
                     EKHVKGAAKGGAKPAAKK"
     gene            1912..2964
                     /gene="motB"
                     /locus_tag="VM692_12810"
     CDS             1912..2964
                     /gene="motB"
                     /locus_tag="VM692_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HUQ53097.1"
                     /translation="MNDETRAPIIIKRVKKVAGGHHGGAWKIAYADFVTAMMAFFLLM
                     WLLGSTAEGDRKGIAEYFQTPLRVAMAGGSGSGDASSVIQGGGEDLTRSAGQVRRADE
                     PAKRQTINLQAARAELARREAERLEGLKQRFEQAIQSNEKLSQFKDQIRLAITAEGLQ
                     VTVVDEQNRAMFDTGGAALKDYTREVMRAIGGLLNDVENAISLAGHTDAVQYAGGERG
                     YSNWELSADRANASRRELVAGGMRENKILRVVGLGSSLPLDAADPLNPMNRRISLVVL
                     NDKTERQIRGLPEEPMEEIQNEAEAAQILGLGEPGDAPADGAEVATPDESPATLPDGA
                     PAGDRLAQPEPNARVL"
     gene            complement(2968..3150)
                     /locus_tag="VM692_12815"
     CDS             complement(2968..3150)
                     /locus_tag="VM692_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HUQ53098.1"
                     /translation="MRQTVRRIWAVLRELTGDDAYERYLAHWHARHAADGAPLDRATF
                     CREEQRRKWDGVRRCC"
     gene            complement(3154..>3963)
                     /locus_tag="VM692_12820"
     CDS             complement(3154..>3963)
                     /locus_tag="VM692_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HUQ53099.1"
                     /translation="PVTVDQMSVLAREMGESTLFARTGGAPSLAVGMASIFSNAFGDT
                     LLSVWYHFAIMFEALFILTTVDAGTRVARFMLQDMLGNIAPPLGRTSWYPSVLICSGL
                     VVAAWGYFLYFGTLDPLGGINSLWPLFGIANQMLAAIALCVATTVIVKSGRIRFAWVT
                     ALPLVWLVAVTTAAAWEKLFSPDLRVGFLSHADDLAAQLASGSLTGAAATQAPTLIFN
                     DRLDAALTFFFLVTVWVLVVETVRISHASLSGRRCPPLAETAHVQTRLAEG"
BASE COUNT          770 a         1253 c         1310 g          630 t
ORIGIN      
        1 gatccaagtg caatccctcg cgccgcggcc cgaactcgcg tccaggccga gcaatagtta
       61 cgcaacggtt atcggccgct ggctacgata cttaagggat cggccgtcag gcgccgttca
      121 ggcgccgtgg agtaagattc agcaatgaaa ctcagggtgt cggccatgcg aagagcgatt
      181 ctggtcgtct gtttgacttt gctcggcagc ggcgtggctc acgcgcagca ggccggccag
      241 cggtacgtcg tcgaccaggc gcagtcggat tttcattggc tcgtctacaa ggccggctca
      301 ctctctcgcc ttggtcacaa ccacacgatt gccacgggcg atctgacagg tagcgtgacg
      361 gtcaacgagc aggaccggaa cgcctcgcaa ttcgagctgt cgttcaacgt cgcgaacctc
      421 gtcgtcgacg acccgatgct gcgtgcgaca ctcggcgcgg acttcgagag cgtgccgacg
      481 gccgacgaca tcgcgggcac gcgcaagaac atgctgacgg acaaggtgct cgacggcgaa
      541 aaatacccga gcattcgcat cctcggcacg gggcccgtcg ggagcgccgg caaacaagag
      601 ctgaaggtca aagtcgagct gctcggccgc acggtcgatc tgacggtgcc gacggaagtc
      661 acgatcgccg gcaacgaagt gcgcgccaaa ggcgagttcg agctgactca cgccgatctc
      721 ggcatgaagc cgttcagcgt catgctgggc gcattgcagg tcggcgagaa cctgtcgttc
      781 acctacgaca tccgcgctcg gcgctagtca gcgcccacca ggcgagggcc actcacggcg
      841 ccgcactcgc gcgccgtctc cgcaactgcg agcgctaagc caaacctgtg cggcgctgcc
      901 cgctgtcgcc gcctcgacgc tgtgattcac agcccacttc gacgctcaag tatcgctgcc
      961 gcgggccgaa aacgggttcg tgaggcgggc atcgcagacc tgccgttgtc aggagtctgg
     1021 atgctagttt ttgtcggcta cgcggtggtg gtcgggtcgg tgttcggggg gttcgccctc
     1081 gccggcggcc atctcggcgc aatgtatcag ccgctcgagc tcctcatgat cggtggcgcc
     1141 gcggttggcg cgttcgttgt cggcaactcg ttcaagacga tcaaagcaac cttcaaagcc
     1201 gtgccgagcc tgtttcaggg ctcgaagtac accaaagcga cctacatgga gctgatggca
     1261 ctgctctatg aggttctcga caaaacgcgc aaggaaggcc tactcgcgat cgagcgcgac
     1321 atcgagtcgc cgaaagacag cgctctcttc aagaagtacc cgagggttct caaggaccac
     1381 cacatggtcg agttcatcac cgactatctg cgactgatgg tcagcggtaa gttgaacgcg
     1441 atcgagatcg aagccttgat ggatcgcgag atcgacacgc atcaccacga gggtgccgag
     1501 cctgcgcacg cgctgcagaa aatcgccgac ggcctgccgg cgttcggcat cgttgcggcc
     1561 gtcatgggcg tcgtgcacac gatggagtcg gtcggcctgc cgccgcagga gctcggaatc
     1621 ctgatcgcgc acgcgctcgt cggcacgttc ctcggcatct tgctgtcgta cggcttcgtt
     1681 gcaccgttgg tcggcgttct ggaaaagaaa gggcacgaag ccagcaaggc gctcgagtgc
     1741 atcaaagtta cgttgctagc gagcttgaac gatgcggtgc cgaccgtcgc cgtggagttc
     1801 gggcgcaagg tactgtactc gacggaacgc ccggggttct cggagctcga aaagcacgtc
     1861 aaaggcgccg cgaaaggcgg cgcgaagccc gcagccaaga agtagtcggg gatgaacgac
     1921 gaaacccgcg cgccgatcat catcaagcgc gtcaagaagg tcgcaggcgg ccaccatggc
     1981 ggcgcgtgga aaatcgctta cgccgatttc gtcacggcga tgatggcgtt cttcctgttg
     2041 atgtggctgt tgggctccac ggccgaaggc gaccgcaagg gcatcgcgga gtacttccag
     2101 acgccgctgc gagttgcgat ggcgggcggc tccggctccg gcgacgcgtc gagcgtcatt
     2161 caaggcggcg gcgaggacct gacgcgcagc gccggccaag tgcgccgcgc cgacgagcct
     2221 gcaaagcggc aaaccatcaa cctgcaagcg gcgcgcgccg agctcgcgcg ccgtgaagcc
     2281 gagcggctcg aaggcttgaa gcagcgcttc gagcaggcaa ttcagagcaa cgagaagctg
     2341 tcgcagttca aggaccagat ccggctcgcg atcacggccg agggtctgca ggtcaccgtc
     2401 gtcgacgagc agaatcgcgc gatgttcgac acgggcggcg cggcgctgaa ggactacacg
     2461 cgcgaggtca tgcgggcgat cggcgggttg ttgaacgacg tcgaaaacgc gatctcgctc
     2521 gcgggtcata ccgacgcagt gcagtacgcg ggtggcgagc gcggttactc gaactgggaa
     2581 ctgtcggccg accgagccaa tgcgtcgcgt cgcgagctcg tcgcgggcgg catgcgcgag
     2641 aacaagattt tgcgcgtcgt cggcttggga tcgtcgttgc cgctcgatgc cgcggatccg
     2701 ctgaacccca tgaatcggcg catcagtctc gtcgtgctga acgacaagac ggagcggcag
     2761 attcgcggct tacccgagga gccgatggag gaaattcaga acgaagccga ggcggcgcag
     2821 attttgggcc tcggcgagcc cggagatgcg cccgccgacg gtgctgaagt tgctaccccg
     2881 gacgagtcgc cggccacgtt gcccgatggc gcccccgccg gcgatcggct cgcccagccc
     2941 gagcctaacg cgcgggtgct ctagcgctca gcaacagcgc cggacaccgt cccatttgcg
     3001 acgctgctct tcgcggcaaa acgtcgcgcg gtcgagcggt gcgccgtcgg ccgcatgtct
     3061 ggcatgccag tgcgcgaggt agcgctcgta ggcgtcgtcg cccgtcagct cgcgcagcac
     3121 cgcccaaatt cgtcgcaccg tctgcctcat tcgtcagccc tcggcgaggc gcgtctgaac
     3181 gtgcgcagtc tcggctagcg gtgggcagcg ccgccccgac agcgacgcgt gggagattcg
     3241 cacggtctct acgacaagca cccagacggt gaccaagaag aagaaggtga gcgccgcatc
     3301 cagccgatcg ttgaaaatca acgtgggcgc ctgggtagcc gcggcccctg tcaacgagcc
     3361 cgacgcgagc tgggccgcga gatcgtcggc gtgcgagaga aagccgacgc gcagatccgg
     3421 gctgaacagc ttttcccaag ccgccgccgt cgtaacggcg acgagccaga cgagcggcag
     3481 cgccgtgacc catgcgaatc gaatgcgccc ggacttcacg atcacggtcg tcgcgacgca
     3541 caaggcgatc gccgcgagca tttgattcgc gatgccgaac aacggccaca ggctgttgat
     3601 gccgccgagc ggatcgagcg tgccgaaata caggaagtaa ccccaggcgg cgacgacgag
     3661 cccgctgcag atcagcacgc tcgggtacca actcgttcgg ccgagcggcg gcgcgatgtt
     3721 gccgagcatg tcttgcagca tgaaacgcgc gacgcgcgtg ccggcgtcga ccgtcgtcaa
     3781 gatgaacagc gcctcgaaca tgatcgcgaa gtggtaccaa accgacagca gcgtgtcgcc
     3841 gaaagcattc gagaagatgc tcgccatgcc gacagccaac gacggcgcgc cgcccgtgcg
     3901 cgcgaacagc gtgctctcgc ccatctcgcg ggcgagcacg ctcatctggt cgacggtaac
     3961 ggg
//
LOCUS       DATLEE010000592         2230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9653028, whole genome shotgun sequence.
ACCESSION   DATLEE010000592 DATLEE010000000
VERSION     DATLEE010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9653028"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VM692_12825"
     CDS             <1..902
                     /locus_tag="VM692_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53100.1"
                     /translation="YAEGPAAGPSSASETTTAEPSTAAAPQADGDVVRLKNGGLLRGK
                     VSELIPGDSVTIVTLTGKVREFPMSEVEYAGPAAQDPKRSAPSVDVSTAQKQPESVSA
                     DIAKPYVTVHGREARVKLVSDTDGVTFYRQTGSAFAVGAGGSAFATGYERICAAPCEV
                     SLPAGTETLALGRGDKAPRAAEPVTLPAGVSEVRGTFQSRAGVRVGGWIILVGGAIVG
                     SVLMATAMDSEQSCDLGVCYETTEFDGARFLAGTLILGAGVGIGLGMGLMRDKALISV
                     HPQRSSAERSLVSARGASFYGSF"
     gene            complement(909..>2230)
                     /locus_tag="VM692_12830"
     CDS             complement(909..>2230)
                     /locus_tag="VM692_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ53101.1"
                     /translation="LLAQVLEVDPENHDALLLWEQLATGSGPYTEEVDDGRTRVRSAS
                     DMLGVASSPPDGFDPNEPLPSYDLEEASVEQAMHSEPGRSLGKRSLSAMDDPFGGPGG
                     DEPLPSFPLSSQADDDLGIGAEQTTDITELARYEDDEDEQEISIVEDFIPPPAPAAAA
                     EPSYEGLEDVLDEAEFFAARGLYDDAKNILLEQLGRTPNHKLVLERLSEIDAQLASSG
                     ESQTKERSQLSDHGNGQGYDVAQSLEALDALEPPSESFAAESARQSLSSTQDIDVDQV
                     FEKFKAGVKAQVADNDSATHYDLGVAYKEMGLLPDAVGEFETAARDAARECMCFAMIG
                     MIYLEQNQLDRAAEAYVRALSATQKTVEQEMSLYYDLGNVYEMKGKNQDALYYFQKIA
                     RRDPGYRDVADRIQQLSPKPAATSHNSARAANDDEFDRVFDDLFESK"
BASE COUNT          335 a          700 c          775 g          420 t
ORIGIN      
        1 cgtacgccga ggggcctgct gcaggcccga gcagcgcctc ggagacgaca acggcggaac
       61 cgagcacggc ggccgcgcca caagcggatg gcgacgtggt gcgtctcaag aacggcgggc
      121 tgctgcgcgg aaaggtcagc gagctgatac ccggcgactc cgtcacgatc gtgaccctca
      181 ccggcaaggt gcgcgagttt ccgatgagcg aagtcgaata cgccggcccc gctgctcagg
      241 atcccaaacg gtcggctcct tccgtcgacg tcagcacggc gcagaagcag ccagaatccg
      301 tcagcgccga tatcgcaaag ccctatgtca cggtgcatgg gcgcgaggcg cgcgtgaagc
      361 tcgtgagcga cacagacgga gtcacctttt accgccagac cgggtccgct ttcgccgtag
      421 gggctggcgg ctcggcattc gcgacgggct acgagcgcat ctgcgcggcg ccgtgcgaag
      481 tctcgctgcc cgcgggtacg gagacgttag ccttgggtcg cggcgacaag gcgccgagag
      541 cggctgagcc ggttacgcta ccggccggcg tatcggaggt gcgaggtact tttcagagcc
      601 gagcgggcgt tcgcgtcggt ggctggatta tcctcgtcgg cggggcaatc gtcggctcgg
      661 tgctcatggc taccgcgatg gacagcgagc agagttgcga tctcggagtg tgctacgaga
      721 cgaccgagtt cgacggggcg aggttcttgg ccggcacgct gatccttggc gccggtgtcg
      781 gcatcggact cgggatgggc ttgatgcgcg acaaggcgct gatcagcgtg catccccagc
      841 gcagctcggc cgagcggtcg ttggtgtcgg ctcgcggcgc ctctttctac ggcagcttct
      901 gagcgacctt acttcgattc gaacaagtca tcgaacacgc gatcgaactc gtcgtcgttg
      961 gcagcgcggg cgctgttgtg tgacgtggcg gcgggcttgg gcgacagttg ctgaatgcga
     1021 tcggcgacgt cgcggtagcc gggatcgcgg cgcgcgattt tctggaagta gtacagggcg
     1081 tcttggttct tgcccttcat ctcgtacacg ttgccgagat cgtagtagag gctcatctct
     1141 tgctcgacgg tcttttgcgt cgccgagagc gcgcgcacgt aggcttcggc ggcgcggtcg
     1201 agctggtttt gctcgaggta gatcatgccg atcatcgcga agcacatgca ttcacgggcg
     1261 gcgtcgcggg ccgcggtctc gaactcgccg acggcgtcgg gaagcaggcc catttctttg
     1321 taggcgacgc ctagatcgta gtgcgtggcg ctgtcgttgt cggcgacctg cgccttgacg
     1381 cccgccttga acttctcgaa tacctgatcg acgtcgatgt cctgcgtcga cgacagcgac
     1441 tgccgcgcac tctcggccgc gaagctctcg gacgggggct ccagcgcgtc gagcgcttcg
     1501 agcgactgcg ccacgtcgta accttggccg ttgccgtgat cgctgagctg cgagcgttct
     1561 ttggtttgac tctcgccgga gctggcgagc tgcgcgtcaa tctcgctcag ccgctcgagc
     1621 acgagcttgt gattgggggt tcgcccgagc tgctcgagca agatgttctt ggcgtcgtcg
     1681 tacagcccgc gcgccgcgaa gaactcggct tcgtccagca cgtcctccag cccctcgtag
     1741 ctgggctcgg cggcggcggc cggcgccggc ggcgggatga aatcctcgac gatggagatc
     1801 tcttgctcgt cttcgtcatc ttcgtatcga gcgagctccg tgatgtcggt ggtctgctcg
     1861 gcgccgatgc ccaggtcgtc gtccgcctgc gacgagagcg ggaagctggg tagtggttcg
     1921 tcaccgccgg gcccgccgaa cggatcgtcc atggccgaca gcgagcgctt gcccaggcta
     1981 cggcccggct cgctgtgcat ggcctgctcc accgacgctt cttcgagatc gtacgagggc
     2041 agcggctcgt tgggatcgaa gccgtccggc ggcgagctcg ccacgcccaa catgtcgctc
     2101 gcgctgcgca cgcgcgtgcg gccgtcgtcg acctcttcgg tgtacgggcc ggagccggtc
     2161 gcgagctgtt cccacaaaag cagcgcgtcg tggttttcgg gatcgacctc gagcacctgc
     2221 gccagcagca
//
LOCUS       DATLEE010000593         4321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7803793, whole genome shotgun sequence.
ACCESSION   DATLEE010000593 DATLEE010000000
VERSION     DATLEE010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7803793"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VM692_12835"
     CDS             complement(<1..634)
                     /locus_tag="VM692_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ53102.1"
                     /translation="MLRDLSRDLRYAARSLARTPGFTVVAVAVLALGIGATSAIFSLV
                     SAVWLKPLPFADEARLVSLWVDLSSLGGPGRLEAPPANYYAWRERAHSFEDMAAVVPD
                     SMNLTGAGEPERLAVVRTTPNLFVTVGLTPILGRTYGPDDGADEPVVISSSFWMRRLG
                     GDPAVVGGTITLDGSTHAIVGVVPPDFRFPYGEKDVYIATLFAPEMQAEQG"
     gene            complement(647..3127)
                     /locus_tag="VM692_12840"
     CDS             complement(647..3127)
                     /locus_tag="VM692_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ53103.1"
                     /translation="MAHRLQCAAMSLLADLLRDFRYAARALARTPGFTVVALTVLALG
                     IGANSAIFSLVSAVWLRPLPFADAERLVTLWTDLTAVGGPPRVEISPGNYSDWQQRAR
                     SFESMAPLEVATFSLTGDGAEPERLTGVRTSSNLFATIGQAPLLGRTFVPEDGTGEPV
                     AVVSEGFWLRRLSGDPAAVGRTITLDGSPHTVVGVVPRDFRFPGESDVFVPTTWSPEV
                     LARDASYYWYLVAKLRPDVSLEAARAELATISAALRDESPNTGRGVAVTVLPLRESLA
                     RGIGFAGPFADSSGTLFALLGAVALVLLIGCANVANLILTRATARQKELAIRKALGAA
                     RGRVLRQLLAESILLAGASVVVGLAIAVACFGYLSRLLPGTLPASATMALDSRVLALT
                     IAAAIVTVLLFGVGPAFAAASRDFSAAFGRAVGAHGKQARRLRTTLVVAEIALTVVLL
                     TGAGLLLRSYTNVLAVDPGVDADGVLVAETVLPEAIASNAAQRAAFYRRVLEELRALP
                     GVESAGYTSFAPLLFKGGRSVVFIDGRPRPERAEMLQHTATNRSASPGYFEALGVPLV
                     SGRFIDERDVRGSQRVVVINESLARAYWPEGDPIGQRLSMGGGEMMTVIGVVGDVRQL
                     GLDVPADRELFMPIDQIDGAFMAPRQLVVRTNGDPLALAAAVRRAVWAVDANQPVSNV
                     RAMSEVLDAELANRDTQLTLLGAFAAIALVLAAVGLYGVLSYTVAQSTNEIGLRMALG
                     AQQRTVVGTVVRSALGAALTGVGVGLVVAYALTRTIASFLYGVSPTDPMTAVAVAGVL
                     LLVAGLAAFVPALRAARVNPLTALRAEA"
     gene            3260..>4321
                     /locus_tag="VM692_12845"
     CDS             3260..>4321
                     /locus_tag="VM692_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUQ53104.1"
                     /translation="MDLDLDPEALFSSDRMELVLRSAQVGVWYCPLPFDRLIWDQRVK
                     EHFHLPPTAEVTIDTFYDRLHPRRPRTNRAAIAHSIDARTQYDIDYRTVSPDGKTTKW
                     IRAVGRTFYDDGGKPIQFDGVTVDVTERKRSEEALHRHVATLANLNATNLALASSTET
                     ETIVQTATDAATQATRAQFGAFFYNVTKPSGEQYMLYTLSGVPRSAFAKFPMPRNTAV
                     FAPTFRGESVVRSDDITRDPRYGQNTPRKGMPDGHLPVRSYLATPVKSRSGEVIGGLF
                     FGHPEPGVFSDRSEQLMSGLAGQAAIGLDNARLFQAAQFANANLEQRVEERTAELQKA
                     EEALRQSQKMEAVGQLTGGL"
BASE COUNT          803 a         1495 c         1428 g          595 t
ORIGIN      
        1 agccctgttc agcctgcatc tcgggcgcga aaagcgtggc gatgtacacg tccttctcgc
       61 cgtagggaaa ccgaaaatcc ggcggcacga cgccgacgat cgcgtgcgtg gagccatcga
      121 gcgtgatcgt tccaccgacg accgcaggat cgccgccgag ccggcgcatc caaaagctcg
      181 agctgatgac gacgggctcg tccgcgccat cgtccgggcc gtacgtgcgc cccaggatcg
      241 gcgtcagccc gaccgtgacg aacaagttgg gtgttgtgcg cacgaccgca agccgctccg
      301 gctctcctgc gcccgtcaag ttcatgctgt cggggacgac cgcggccatg tcctcgaacg
      361 agtgcgcacg ctcgcgccag gcatagtagt tggcgggcgg cgcctcgaga cgcccggggc
      421 cgcctagcga cgacagatcg acccatagcg acacgagccg agcctcgtcg gcgaacggca
      481 gcggcttcag ccacaccgcg ctaaccaagc tgaagatcgc agacgtggcg ccgatgccga
      541 gcgcgagcac ggcgacggcg acgacggtga agccgggagt gcgggccagc gagcgcgcgg
      601 cgtaacgcaa atcgcgtgag agatcgcgga gcatgagcgg actcccctac gcttcggcgc
      661 gcaacgccgt cagcggattc acgcgcgctg ctcgcagcgc gggcacgaac gctgcgagcc
      721 ccgcgacgag cagcagcaca ccggcgaccg cgactgcggt catgggatcc gtcgggctca
      781 caccgtacaa gaacgacgca atcgtgcgcg tcaacgcata cgccacgacg agccccacgc
      841 cgacaccggt taacgccgcg ccgagtgccg agcggacgac ggttccgacg accgttcgct
      901 gttgcgcgcc gagcgccatg cgcaggccga tctcgttggt gctctgcgcc acggtatagc
      961 tcaacacgcc gtagagaccc acggccgcta gcaccagcgc aatcgcagcg aaggcgccga
     1021 gcagcgtcag ctgcgtgtcg cgattcgcaa gctcggcgtc gagcacctcg ctcatggcac
     1081 gcacgttcga caccggctga tttgcgtcga cggcccagac cgcacggcgc accgcagcgg
     1141 ccaacgcgag cggatcgccg ttcgtgcgga cgacgagctg ccgcggcgcc atgaaggccc
     1201 cgtcgatctg gtcgatcggc atgaacagct cgcgatcggc gggcacgtcg agccccagct
     1261 gacgaacgtc tccgactacg ccaatcaccg tcatcatttc gccgccgccc atgctgagcc
     1321 gctggccgat cgggtcgcct tccggccaat aggcgcgcgc aaggctctcg ttgatgacga
     1381 cgacgcgctg cgagccgcgc acgtcgcgct cgtcgatgaa ccggccgctc acgagcggca
     1441 cgccgagcgc ctcgaagtaa ccagggctcg cgctgcggtt cgtcgcggta tgttgcagca
     1501 tctcggcgcg ctcgggccgc gggcgcccgt cgatgaatac gaccgagcgg ccgcctttga
     1561 acaacagcgg cgcgaagctc gtgtaacccg cactctcgac gccgggcagc gcgcgtagct
     1621 cctcgagcac gcggcgatag aacgccgcgc gctgcgcggc gttcgacgcg atagcctcgg
     1681 gcagcacggt ctccgcgacg agcaccccgt cggcgtcgac accagggtcg acggccagca
     1741 cgttcgtgta gctgcgcagc agcaacccgg ctccggttag caggacgacc gtcagcgcga
     1801 tctcggcgac gacgagcgtc gtgcgcaggc ggcgagcttg cttgccatgc gcgccgacgg
     1861 ctctgccgaa tgcggcgctg aagtcgcggc tcgcggctgc gaaggccggc cccacgccga
     1921 acaacagcac ggtgacgatc gccgctgcga tcgtcagcgc gagcacgcgc gagtcgagcg
     1981 ccatggtcgc gctcgcaggc agcgtgccgg gcagcaggcg cgacaagtaa ccgaagcagg
     2041 ccaccgcgat cgcgaggccg acgacgacgc tggcgcccgc cagcaggatg ctctcggcca
     2101 atagctgccg cagcacgcgg ccgcgcgcgg caccgagcgc tttgcggatc gcgagctcct
     2161 tctgccgcgc cgtggcgcgc gtgaggatca agttcgcgac gttggcgcag ccgatcagca
     2221 acacgagtgc gacggcgccg agcaacgcga acagcgtgcc ggagctatcg gcgaacggtc
     2281 ctgcgaaacc gatgccgcgc gcaagcgact cccgcaaggg aagcacggtg acggcgactc
     2341 cgcggcccgt gttcgggctc tcgtcccgta gggcggcgga gattgtggcc agttccgcgc
     2401 gcgcggcctc cagcgagacg tcgggccgca gcttcgcgac gagataccag tagtacgacg
     2461 cgtcgcgcgc cagtacctcg ggcgaccacg tcgtcggaac gaacacgtcg ctctcccccg
     2521 gaaaacgaaa atcccgcggc acgacaccga cgaccgtatg gggggagcca tctagtgtga
     2581 tcgtgcggcc gacggccgcg gggtcgccac ttaagcgacg taaccagaat ccttcgctga
     2641 cgacggcaac gggctcaccc gtgccgtcct cggggacgaa cgtgcggccg agcaacggcg
     2701 cttgaccgat cgttgcgaac aggttcgacg acgtgcgcac gcccgtgagc cgctcgggct
     2761 cggcgccgtc gcccgtcaag ctgaaagtcg cgacctcgag tggcgccatc gactcgaacg
     2821 atcgcgcgcg ctgctgccag tcggagtagt tgccgggcga gatctccacg cgcggcggtc
     2881 caccgaccgc cgtgaggtcg gtccacaacg taacgaggcg ctcggcatcg gcgaacggca
     2941 gcggtctcag ccacaccgcg ctgacgaggc tgaagatcgc cgagttggca ccgatgccga
     3001 gcgcgagaac ggtgagcgct acgaccgtga accccggcgt tcgcgcgagc gcacgcgccg
     3061 catagcggaa gtcgcgcagc aggtcggcca ataggctcat ggccgcgcac tgcaatcgat
     3121 gtgccatgac gcaaatcgtg aagaatcgcg ccgttgcaca cgaaggcaca tagcgtttgt
     3181 cccgatagcg agcggcgtga tcccgcttcc gggaccggcg ccgcgcccct atgatgctcg
     3241 gccaatggag acctcgaaga tggacctcga cctagatccc gaagccttgt tcagcagcga
     3301 ccgcatggag ctcgtgctcc gcagcgcgca ggtcggcgtc tggtactgcc cgctgccgtt
     3361 cgaccggctg atttgggatc agcgcgtcaa ggagcacttt cacctgccgc caacggccga
     3421 agtgacgatc gacacgttct atgatcgttt gcacccccga cgaccgcgaa cgaaccgcgc
     3481 cgcgatcgcg cacagcatcg acgcgcgcac gcagtacgac atcgattacc gcaccgtgtc
     3541 gcctgacggt aagaccacga agtggattcg cgcggtcggc aggacttttt atgacgacgg
     3601 cggcaagccg atccaattcg acggcgtcac cgtcgacgtc acggagcgca agagatcgga
     3661 ggaggcgtta caccggcacg tggcgacgct ggccaacctc aacgcgacga accttgcctt
     3721 ggcgtcgtcg accgagaccg agacgatcgt gcagaccgcc accgacgcgg caacgcaggc
     3781 gacgcgagcg caattcggcg cgttcttcta caacgtcacc aagccgagcg gcgaacagta
     3841 catgctgtac acgctatcgg gcgtgccgcg ctcggcgttc gccaagtttc cgatgccgcg
     3901 taacacggcc gtgttcgcgc cgacgttccg cggcgagagc gtcgtccgct cggacgacat
     3961 cactcgcgat ccgcgctacg gtcaaaacac cccgcgcaag ggcatgcccg acgggcacct
     4021 acccgtccgc agctacctgg ccacgcccgt gaaatcgcgc tcgggcgaag tcatcggcgg
     4081 cttgtttttc ggccacccag aaccgggcgt attcagcgat cgctccgaac agcttatgag
     4141 tggcctagct gggcaggccg ccatcggcct cgacaacgct cgcctttttc aagcggcaca
     4201 attcgccaat gcaaacctag agcagcgggt ggaggagcga acagcggagt tgcagaaggc
     4261 cgaagaagcc ttgcgtcagt ctcaaaagat ggaagccgtt ggccagctca cgggcgggct
     4321 g
//
LOCUS       DATLEE010000594         5497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2203795, whole genome shotgun sequence.
ACCESSION   DATLEE010000594 DATLEE010000000
VERSION     DATLEE010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203795"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1140)
                     /locus_tag="VM692_12850"
     CDS             complement(10..1140)
                     /locus_tag="VM692_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53105.1"
                     /translation="MHRRIALGFSAALFCTVNASAQNAANAPAAVPAEQTAAAATNKN
                     WRAPKFSWGHPNLEGTFTSRDMGGIPLQRPDEFGTRQFLTQEEFQARVTAGPSGFAAF
                     VNQQSEDASRLELSALDSTETGTRVFGYTSYVIEPLDGKVPAFTEEGRQRQARVRPTN
                     GPYHTTADFSYYDRCITRGVTGSIMPSLYGDAMRIIQSPTEVAISYEMLHDTRVVPLD
                     GRAQASEGVRQYMGSSVGHFEGNTLVVETTNFTDELSVGRMPHSEEMKLTERFTRIDP
                     DMLDYVVTVDDPRTYTAPWTFRLTLTTQPGYEVLEYSCHEGNNFIRTALRAEQQYQQR
                     VAAARDKGEPIPERPRARNTGGGLGIFAPPSETEAVEIGTAQ"
     gene            1329..2114
                     /locus_tag="VM692_12855"
     CDS             1329..2114
                     /locus_tag="VM692_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53106.1"
                     /translation="MSILAQPVLGQQRGRPERIGGKPNLNGIWQAANEAYWNLEGHSA
                     QALDEFSKKMGAVAAIPAGQSVVVGGSIPYLPAALAKRDQNRAGWPASDPEAKCYMPG
                     IPRATYMPYPFQIVQGEGDQILFSYTFANANRPVFMRDHQRAPVDLWMGRSNGAWDGD
                     TLVIEVNSNIENTWFDRAGNHHSAAMIVTERYTLVDEHMLQYEATIEDPETFSRPWTI
                     RMPLYRRAEDNARLLEFNCVKFAEPLLYGDFTSRDETQPADRR"
     gene            2157..2567
                     /locus_tag="VM692_12860"
     CDS             2157..2567
                     /locus_tag="VM692_12860"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53107.1"
                     /translation="MSRHSLACAALVAALAALAAPALAHHSFAAEFDLNQPVELKGEV
                     IRMVFTNPHSRIFLKVTTATGEVQEWSIEGGAPNALIRRGWNRDSLPPGTVVTVLGYR
                     ARDGSLSASGGSVKLPDGSHLDATSGGIGAPSAE"
     gene            2663..3304
                     /locus_tag="VM692_12865"
     CDS             2663..3304
                     /locus_tag="VM692_12865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HUQ53108.1"
                     /translation="MEVLRLFPTPIVVATIPNAEALNAELRSIILARERANASIERSN
                     QGGWQSSWDMHEWGGAPMQKVVAHARAVVDDATVDRAGNRPKIAWRINSWANVSRLGH
                     GNQFHTHPGALWSVSYYVDDGGIGNDPSLGGEFEIRDPRGVAPIMYAPQLTFPGADFV
                     ALGESQRLAPRAGVLIVFPSWLSHGVCPYRGNAERISIAINFSIANIQANVPG"
     gene            3370..4356
                     /locus_tag="VM692_12870"
     CDS             3370..4356
                     /locus_tag="VM692_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53109.1"
                     /translation="MQLRSTLVIALGLVLGSAAVPARAQTAPAPDAQAQAAERQRQQD
                     AMPDTRGTGHWAALKEIDPGLAEHVIYRPANLDALGATKLGVYVFGNGGCTDDGASAR
                     LHLLEVASHGYLAIAPGRIYNGPNKTQRPAQRPAPAGPGGPAATVAKELREALDWALA
                     ENARRDSRYFGRIDPRAVAISGYSCGGIQALANAKDPRVATVIIMNSGLLNTGPTRMG
                     GVEAGKELLADLHTPTLYILGGPTDIAYANGMDDFARIAHVPVAAANIDKGHGGTYWE
                     PNGGAAAQVAVQWLDWRLRSDATAGRMFVGTQCGLCTDAKWKLETKGFDASR"
     gene            4410..5183
                     /locus_tag="VM692_12875"
     CDS             4410..5183
                     /locus_tag="VM692_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUQ53110.1"
                     /translation="MVLLSAVCNAGLAQPAQGQGGTVLITGSNRGLGLELAKQYAAAG
                     WRVIATARDPREAAELQALAAGDADVRVEELDVLDASELKALVAKLDGVPIDVLINNA
                     GVLGSQAGQTLGSLDYAEFERVMGVNVYAPLAIADALRDNVAASRDKKIVAITSRSGI
                     ISQPGFGGPPFYRASKIALNMTMHVLAAQLRERGVIVALVAAPPTDTDMLRELIGRDN
                     AARMTRPADAVAGLIRVIGGLTLDNSSLPLYYDGTAMAW"
     gene            complement(5198..>5497)
                     /locus_tag="VM692_12880"
     CDS             complement(5198..>5497)
                     /locus_tag="VM692_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HUQ53111.1"
                     /translation="PLGGYELTVGPITDPNLPWVLLGRALLHAKLVAERNHSVREGLV
                     VDAQAGSHLANAIDAAPRRKLDLAFRRLRGGGGIEAEEREALVAELAPLIDPRAG"
BASE COUNT          891 a         1851 c         1867 g          888 t
ORIGIN      
        1 ggtagcggcc tattgagccg tgccgatctc gacggcctcg gtctcggacg gcggcgcgaa
       61 gatgccgaga cccccgcccg tgttgcgggc gcgcgggcgc tcgggtatcg gctcgccttt
      121 gtcgcgtgca gcggcgacgc gctgctgata ctgctgttcc gcgcgcagcg ccgtgcgaat
      181 gaagttgtta ccctcgtggc acgagtactc gagcacctcg tagccgggct gcgtcgtcag
      241 cgtcaggcgg aatgtccagg gcgccgtgta ggttcgcggg tcgtcgacgg tcacgacgta
      301 gtcgagcatg tcggggtcga tgcgcgtgaa gcgctccgta agcttcatct cctcgctgtg
      361 cggcatgcgg ccgacgctga gctcgtccgt gaagttggtg gtctcgacga cgagcgtgtt
      421 gccttcgaag tggccgaccg acgatcccat gtattggcgg acgccttcgc tcgcctgcgc
      481 acgcccgtcg agcggaacga cgcgcgtgtc gtgcagcatc tcgtagctga tggcgacctc
      541 ggtcggcgac tgaatgatgc gcatcgcgtc accgtagagc gacggcatga tcgagcccgt
      601 aacgccgcgc gtgatgcagc ggtcgtagta cgagaaatcc gccgtcgtgt ggtacggccc
      661 gttcgtcggc cgcacgcgcg cttgccgctg ccgtccttct tcggtgaaag ccggcacctt
      721 gccgtccaaa ggctcgatga cgtacgacgt gtagccgaac acgcgggtgc cggtctcggt
      781 cgagtcgagc gcgctcagct cgagccggct cgcgtcttcg gactgttggt tgacgaaggc
      841 cgcgaagccg ctcgggccgg cggtcacgcg cgcttgaaac tcttcttgtg tcaggaactg
      901 acgcgttccg aactcgtcgg ggcgctgcag cgggatgccg cccatgtcgc ggctcgtgaa
      961 cgtcccctcg agattcgggt ggccccacga gaacttgggc gcgcgccagt ttttattggt
     1021 cgcagcggcc gcggtttgct ccgcggggac cgcggcgggc gcgttagccg cgttctgcgc
     1081 gctcgcgttc acggtgcaga agagggcggc cgaaaagccg agcgcgatgc gcctgtgcat
     1141 gtgttctcca gctgcgactt tcaggtagag taatttgaat gcgccgaaaa attccagcaa
     1201 tcccccttcg gggacttcca agggtcgtcc cccttcgggg atttccaagg gtcatccccc
     1261 ttcgcggact tccaagtgtc gtcccccttc ggggatttcc aagggtcata gccgccttca
     1321 tcctgtcctt gtccattctc gcgcaacccg tgctcgggca gcagcgcggc cggccggagc
     1381 gcatcggcgg caagccgaac ttgaacggca tctggcaagc cgccaacgag gcgtactgga
     1441 atctcgaggg ccattcggcg caagcgctcg acgagttctc gaagaaaatg ggcgctgtcg
     1501 cggcgattcc ggccgggcag agcgtcgtcg tcggcggctc gatcccctat ctgccggcgg
     1561 cgctcgccaa acgcgaccag aaccgcgccg gctggccggc ctccgacccc gaggccaagt
     1621 gctacatgcc gggcatccca cgcgcgactt acatgccgta cccgttccag atcgtgcagg
     1681 gcgagggcga ccagatcctg ttctcgtaca cgtttgcgaa cgcgaaccga ccggtgttca
     1741 tgcgcgacca tcaacgagcg cccgtcgacc tctggatggg gcggtcgaac ggcgcctggg
     1801 acggcgacac gctcgtcatc gaggtcaact cgaacatcga gaacacctgg ttcgatcgcg
     1861 cgggcaacca ccacagcgcg gccatgatcg tgaccgagcg ctacacgctc gtcgacgagc
     1921 acatgctgca gtacgaggcc acgatcgagg atccggaaac cttcagccgg ccgtggacga
     1981 tccgtatgcc gctgtatcgg cgcgccgagg acaatgcgcg cttgctcgaa ttcaactgcg
     2041 tgaagttcgc tgagccgctg ctctacggcg acttcacgag ccgggatgag acgcagccgg
     2101 ccgaccgtcg ctagcttgcg agcagaatgc caaggtcatc attgggaggc gattcgatgt
     2161 cacgacactc tctggcttgc gccgcgctcg tcgccgcgct cgcggctctg gcggcgcccg
     2221 cgcttgcaca tcactcgttt gcggccgagt tcgacctcaa tcagcccgtc gagctcaagg
     2281 gcgaggtcat ccgcatggtc ttcacgaacc cgcattcgcg gatttttctg aaggtcacga
     2341 ccgcgaccgg cgaggtccag gagtggtcga tcgagggcgg agcgccgaac gcgctgattc
     2401 gacgcggctg gaaccgcgac tcgctgcctc cgggcaccgt cgtcacggtg ctcggctatc
     2461 gcgcgcgcga cgggtcgctc tcggccagcg gcggctcggt caagctgccc gacggctcgc
     2521 acctcgacgc tacgtccggc ggtatcggcg ccccgagcgc ggagtagcca ctgccatcgc
     2581 tcgcgcgccg tcgcgcgcga acgtttttca cgcccgaggt tcgcggtcgc acttcgctcg
     2641 cgcgataatc gagccgatgc aagtggaagt gctcaggctg ttcccgacgc cgatcgtcgt
     2701 cgcgaccatt ccgaacgccg aggcgctgaa tgccgagctc aggagcatca tcctcgcgcg
     2761 tgagcgcgcg aacgcatcca tcgaacgctc gaaccagggc ggctggcagt ccagctggga
     2821 catgcacgag tggggcggtg ctccgatgca gaaggtcgtg gctcatgccc gagccgtcgt
     2881 cgacgacgcc accgtggacc gtgccggcaa ccgcccgaag atcgcgtggc gcatcaactc
     2941 ttgggcgaac gtcagccgcc tcgggcacgg caatcagttt catacgcatc ctggcgcgct
     3001 ttggtcggtc agctattacg tcgacgatgg cggcatcggc aacgatccgt cgctcggcgg
     3061 cgagttcgag attcgggacc cgcgcggcgt ggcgccgatc atgtacgcgc cgcagctgac
     3121 gttccccggg gcggatttcg tcgcgctcgg cgagtcgcag aggctcgctc cgcgcgccgg
     3181 cgtgctgatc gtgttcccga gttggctgtc gcacggcgta tgtccttatc gcggcaacgc
     3241 cgagaggatc tcgatcgcca tcaacttcag catcgccaat atccaagcaa acgtgccggg
     3301 atgacggccg ataggcgcgc gacgtgccgc ggcggtacag ttacgcgcag ctcagcgaag
     3361 ggggacgcca tgcaacttcg atccactctc gtcatagcgc tcggcctcgt gctcggcagc
     3421 gccgcggtgc ccgcgagggc gcagacagcg cctgcgccgg acgctcaggc acaagccgcg
     3481 gaacggcagc gccagcagga cgcgatgccc gatacgcggg gcacggggca ttgggccgcg
     3541 ctcaaggaaa tcgatcccgg cttggcggag cacgtgatct accggccggc gaacctcgac
     3601 gcgctcggcg ccacgaagct cggcgtctac gtgttcggca acggcggttg caccgacgac
     3661 ggcgccagcg cgcgcttgca cctgctcgag gtcgcgtcgc acggctatct cgcgattgcg
     3721 ccaggccgca tctacaacgg tcccaacaaa acgcaacggc ccgcgcagcg tcccgcaccg
     3781 gcgggccccg gtggccccgc ggcgacggtc gccaaggagc tgcgcgaggc gctcgactgg
     3841 gcgctcgcgg aaaacgcgcg ccgcgacagc cgctacttcg gccgcatcga cccgcgagcc
     3901 gttgcaattt ccgggtacag ctgcggtgga atccaggcgc tcgccaacgc caaggatccg
     3961 cgcgtggcga cggtgatcat catgaacagc ggcctgctca acacggggcc gacgcgcatg
     4021 ggcggcgtcg aggccggcaa ggagctcctc gccgacctgc atacgccgac gctgtacatc
     4081 ctgggcggcc ccacggacat tgcgtacgcg aacggcatgg acgacttcgc gcgcatcgcg
     4141 cacgttcccg tcgcggcggc gaacatcgac aagggacacg gcggtacgta ttgggagccg
     4201 aacggcggcg ccgccgcaca ggtcgccgtg cagtggctcg actggcgctt gcgcagcgac
     4261 gcgaccgcgg gccgcatgtt cgtcggcacg cagtgcggcc tctgcaccga cgcgaagtgg
     4321 aagctcgaga caaaggggtt cgacgcgtcg cgctgagctc gggacgaagg ggagagcgag
     4381 cgatgcgaag cggctgggtt gcggtttgcg tggtcttgct ttcggcggtc tgcaacgcgg
     4441 gcctcgcgca gccggcgcaa gggcaaggcg gcaccgtgct gatcacgggc tcgaaccgcg
     4501 ggctcggcct cgagctcgcg aagcagtacg ccgcggcagg ttggcgcgtg atcgcgacgg
     4561 cgcgcgatcc ccgcgaagcg gccgagctgc aggcgctcgc ggccggcgac gccgacgtgc
     4621 gcgtcgagga gctcgacgtg ctcgacgcaa gcgagctgaa ggcgctcgtc gcgaagctcg
     4681 acggcgtgcc catcgacgtg ctgatcaaca acgccggcgt gctcggttcg caggccggcc
     4741 agacgctcgg ctcgctcgac tacgcggagt tcgagcgcgt gatgggcgtg aacgtctacg
     4801 cgccgctcgc gatcgcggac gcgttgcgcg acaacgtcgc cgcgagccgc gacaagaaga
     4861 tcgtcgccat cacgagccgc tccggcatca tttcgcagcc gggtttcggc ggcccaccgt
     4921 tctatcgagc gagcaagatc gcgctgaaca tgacgatgca cgtgctggcg gcgcagctac
     4981 gggaacgcgg cgtgatcgtc gcgctcgtcg cggcgccgcc gaccgacaca gacatgctgc
     5041 gcgagctgat cgggcgcgac aacgccgcgc gcatgacgcg gcccgccgat gccgtcgcgg
     5101 gtctcatccg ggtcatcggc ggcctgacgc tcgacaacag ctcgctgccg ctctactacg
     5161 acggcacggc catggcctgg tgaacgagcg actcggctca gcccgctcgc ggatcgatca
     5221 acggagcgag ctccgcgacc agcgcctcgc gttcttctgc ttcgatgcct ccgcctccgc
     5281 gcaagcgccg aaacgcgaga tcgagcttac gccgcggcgc ggcgtcgatc gcgttagcca
     5341 agtggctgcc ggcttgcgcg tcgacgacga gcccttcgcg tacagagtgg ttgcgctcgg
     5401 caacgagctt cgcatgcagc agcgcgcggc cgagcaacac ccacggcaaa ttcggatccg
     5461 tgatcgggcc gaccgtcagc tcataaccgc cgagcgg
//
LOCUS       DATLEE010000595         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5186261, whole genome shotgun sequence.
ACCESSION   DATLEE010000595 DATLEE010000000
VERSION     DATLEE010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5186261"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VM692_12885"
     CDS             <1..1157
                     /locus_tag="VM692_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUQ53112.1"
                     /translation="LVLRGDAGAGVQELAADLVDNDLGASRSPDSQRLKFGIDESGAR
                     VALEPYGGCILICGASASGKSTVARRIVESLQQLTYQLCIVDPEGDYEQQPGAVVIGK
                     PDAEPRREEVMQLLEDHGVNAVVSMTGVPISDRPPYFAALLSDLLQLRVRYGRPHWLV
                     IDEAHHLFPAEWQIPNGLLPASLTNVLLVTVHPELLSTELHKRVTQLVAVGPTAPELL
                     RQFAALRGLTLTERALAAAMAAPVAVGEVLLWSPADETCRRVRLEPPSAEQRRHTRKY
                     AVGQLPPDRSFYFRGAAGKLNLRAQNLLQFLEIAAGVDTETWAHHFRQGDYSRWFRDV
                     IKDTELAAAAAAVERNRFASREDALAELRAAVESRYTLPAEAPLPVPGAS"
     gene            complement(1167..1289)
                     /locus_tag="VM692_12890"
     CDS             complement(1167..1289)
                     /locus_tag="VM692_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53113.1"
                     /translation="MALTGYGQPDDRDEARRAGFDDHLVKPADLQSVDAILAES"
     gene            1309..1587
                     /locus_tag="VM692_12895"
     CDS             1309..1587
                     /locus_tag="VM692_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53114.1"
                     /translation="MSLAGEPPRDLVAVHLRQPDVDERDLGVQALRHLERGAAAVCRG
                     DLVAFQLEQHRERVRCVGAVVDDENAARPAAAANGSLASALRCAASGS"
     gene            complement(1648..2313)
                     /locus_tag="VM692_12900"
     CDS             complement(1648..2313)
                     /locus_tag="VM692_12900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HUQ53115.1"
                     /translation="MRGDDWSRFVHPADRDEYLNAFQRAFATQTRFTAEFRFRRHDGE
                     YRWMRSDATPRIGDNGALEGYVGATVDITERRNAEDALRIADRQKDEFLAMLAHELRN
                     PLAPIRNASEVLALRYAGDPQAAGPIAMLRRQTDHLARLLDDLLDVTRIAQSRVLLKR
                     EPLEIGAVIDQAAETVAPLASAKSQTLRVDRDAAPLYVNGDRTRLVQSLTNVASACRL
                     SSA"
     gene            complement(2353..>2729)
                     /locus_tag="VM692_12905"
     CDS             complement(2353..>2729)
                     /locus_tag="VM692_12905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HUQ53116.1"
                     /translation="FGFTAEQPATRAALLERVLPSDRARLEAAVAAAADVAGTRRYSV
                     EYRIVRGGDGAVRWIANTGFAVFENDRLVRIVGTARDITDHHRVETALRESEQRFATL
                     AEGTSVLLWVNGHDGNEFVNRA"
BASE COUNT          414 a          952 c          916 g          447 t
ORIGIN      
        1 acttggtgct gcgcggcgac gccggcgccg gtgtacagga gctcgcagcc gacctcgtcg
       61 acaacgatct aggcgcgagc cgctcgcccg atagtcaacg cctgaagttc ggcatcgacg
      121 aaagcggcgc gcgcgttgcg ctcgaaccct acggcggctg catcctgatc tgcggcgcgt
      181 cggcgagcgg caaatccacg gtcgcgcggc gcatagtcga gtcgctgcag caactgacgt
      241 atcagctctg catcgtcgac cccgagggcg actatgagca gcaaccgggc gccgtcgtca
      301 tcggcaagcc cgatgccgag ccgcggcgcg aagaagtcat gcagctgctc gaggaccatg
      361 gcgtcaacgc cgtcgtgtcg atgacgggcg tgccgatctc cgatcgaccg ccctacttcg
      421 cggcgctgct gtcggacttg ctacagctgc gcgtgcggta cggccggccg cattggctcg
      481 tcatagacga ggcgcatcac ttgttcccgg ccgagtggca aatcccgaac ggcttgctgc
      541 cggcgtcctt gacgaacgtg ctgctcgtca ccgtccaccc ggagctgctg tcgaccgagc
      601 tgcacaagcg agtgacgcag ctcgtcgccg tcggacccac ggcgcccgag ctgctgcggc
      661 aattcgccgc gcttcgcggc ctgacgttga ccgagcgtgc cctcgcggcg gcaatggcgg
      721 cccccgtcgc agtcggcgag gtcttgctct ggtcgcccgc cgacgaaacc tgtcgacgcg
      781 tgcgcttgga gccgccgagc gccgagcaac gccgccacac gcgcaagtat gccgtcggcc
      841 agttgcctcc cgatcgcagc ttctactttc gcggcgccgc cggaaagctg aatctgcgcg
      901 cgcaaaatct gctgcagttt ctcgaaatcg ccgccggcgt cgacaccgag acctgggctc
      961 atcacttccg gcaaggcgat tactcccgct ggtttcgcga cgtcatcaag gacaccgagc
     1021 tcgctgcggc cgcggcagcc gtcgagcgca atcggtttgc atcacgcgag gacgccttag
     1081 ctgagctgcg agcggccgtc gagagccgct acacgttacc ggccgaggcg ccgctgccgg
     1141 ttcccggcgc gagctgacgc gcgcgctcag gactctgcca ggatcgcgtc gacggactgc
     1201 agatccgcgg gcttgacgag atgatcatcg aagccggcgc ggcgggcttc gtcgcgatcg
     1261 tcgggttggc cgtagcccgt cagggccaca agtcgaatcg cgcgccccgt gtcgctggcc
     1321 ggggagccgc cgcgcgacct cgtagccgtc catttgcggc aacccgatgt cgatgaacgc
     1381 gacctcgggg tgcaggcgct ccgccacctc gagcgcggcg ctgccgctgt atgccgtggc
     1441 gacctcgtgg ccttccagct cgagcagcat cgcgagcgag tccgctgcgt cggcgctgtc
     1501 gtcgacgacg agaacgcggc gcgcccggcc gcggcggcga acggctcgct tgcgtcggcg
     1561 cttcgatgcg cggcaagcgg atcgtgaccg tcgtgccgcg cccatgaccc tcgctttcga
     1621 cgccgactgc tccgccgtgc atttcgatca ggcgcttgac aaccgacaag ccgatgcgac
     1681 gttcgtgagg ctctgcacga gccgcgtgcg gtcgccgttg acgtacagcg gcgccgcgtc
     1741 gcggtcgaca cgcagtgtct gcgacttggc gctcgcgagc ggtgcgacgg tctcggcggc
     1801 ttgatcgatg acggcgccga tctcgagcgg ctcgcgcttc agcagcacgc ggctctgagc
     1861 gatgcgcgtg acgtcgagca ggtcgtcgag cagccgcgcg aggtgatcgg tctgccttcg
     1921 caacatcgcg atcggcccgg cggcctgcgg atcgcccgcg tagcgcagcg ccagcacctc
     1981 gctggcgttg cgtatcggcg ccagcggatt ccgcagctcg tgcgcgagca tcgcgagaaa
     2041 ctcgtctttt tgccggtcag cgattcgtag ggcgtcctcg gcgttgcgcc gctccgtgat
     2101 gtcgaccgtg gcgccgacgt agccctcgag cgcgccgttg tcgccgatgc gtggcgtcgc
     2161 gtcggagcgc atccaccggt attcgccgtc gtggcggcga aagcgaaact cggccgtgaa
     2221 gcgcgtctgc gtcgcaaaag cgcgctgaaa ggcattcagg tactcgtcgc gatcggccgg
     2281 atgcacgaag cggctccaat cgtccccgcg cacgtccctg tcggtgtcgg acccgacgcc
     2341 aacgaagtcg agttaggcgc ggttgacgaa ctcgttgccg tcgtgcccat tgacccagag
     2401 caacaccgaa gttccttctg ccagcgtcgc aaagcgctgc tcgctctcgc gcagcgcggt
     2461 ttccacgcgg tgatgatccg tgatgtcgcg cgcagttccg acgatgcgca cgagccgatc
     2521 gttctcgaag acggcaaacc ccgtgttggc tatccaacgc acggcgccgt cgccgccgcg
     2581 cacgatgcga tactccacgg agtagcgccg cgtgcccgcg acatcggctg ccgcggcaac
     2641 ggccgcttcg agacgcgcgc gatcgctcgg aagcacgcgc tccagcaacg cggcgcgcgt
     2701 cgccggctgc tcggctgtga agccgaata
//
LOCUS       DATLEE010000596         2638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4418753, whole genome shotgun sequence.
ACCESSION   DATLEE010000596 DATLEE010000000
VERSION     DATLEE010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4418753"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..2563
                     /locus_tag="VM692_12910"
     CDS             284..2563
                     /locus_tag="VM692_12910"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HUQ53117.1"
                     /translation="MPSPERLTSLRTTLVAVVTLLIGAPALGQELSIPPVVAPPTMAD
                     FVGMNPSEDVRSRYAYVTGFTQRTPQNGAPSAQRTDVYLGYDSRNLYAVFLAFDDDPA
                     SVRANLAPRENIDNDDNLGLVIDTFDDQRTGYGFYSSPLGVQWDGRWSDVTRGGWDTS
                     YEAVWQTDAQLTDGGYVVVMTIPFRAMRFPETSEQRWRVQFERIIPRLSEESQWPAWS
                     QAIDGRLNQTAVVTGVRDVSPGRNVQLIPFAFVRSYDVLDGGRAGGPGFTKDTEDDFG
                     LDAKVVLKDSFVLDFTYNPDFSQVESDQPQVTVNERFEVQFPERRPFFLENADYFAAE
                     TPLLFTRRIVDPKVGLKFTGRQGPWGIGTMLINDEAPGQRAPPTDPLYGEDADISVLR
                     VFRDFGDQSRAGFMHTERELGNTYSRVSAADTYIKLTDNWLTELLMVNTENRLADGRV
                     ITGRETNWRVNRNSRNFNAHYHYTAQSSNFAVPLGILSRNYTPDAEGLHGFMEYRFWP
                     EDTWIDRIGPRVFFQHQEDRSGTRLYSEFSPQLQIAWAGASFFAVGTNDIRERLRPRD
                     FVGLTTTRDYAQTRWFTNISSDPLQTFGFSFNYDQGTVINFVPPLGAEPELADRDYIE
                     GSIRWRPIDRLRIDTTLLSTTLDDRNGRGKIFDDRILRTRVNYQFSKEMSLRVILQHE
                     ETTPTALSRLTNDENLNLDLLFRYVLNPWSALYVGYNNNESNLQLVDTPQGTQLLRSE
                     DLARDGQQFFVKFSYLLQP"
BASE COUNT          478 a          880 c          816 g          464 t
ORIGIN      
        1 agcgacgccg ggccgctgta tctgacgacg gtgctcgcgc gggcggcggg cgagggcaag
       61 ctcatggcca ttcacacgcg cgtcgagtat tcgccgaccg tgatcccggg cttcacgtcg
      121 cgtcccgaga tgtatctcgg gacatgcacg atcgcgagct agtagttttt ctactcgccg
      181 cgcgacgcga caacccgaag ttcggcgagc ctcgccgcat gccggcaacc ctgcgactct
      241 cgcgtgctat tctccgccgc tctttgcccg tcgcggactc tcaatgccga gccctgagcg
      301 cctgacctcg ttgcgcacga cactcgtcgc tgtcgtcact ctgttgatcg gcgctccggc
      361 gctaggccaa gagctcagca ttccgccggt cgtggcaccg cccacgatgg ccgacttcgt
      421 aggcatgaat cctagcgagg acgttcgtag ccgctacgca tacgtcacgg ggttcacgca
      481 gcgcacgccg cagaacggcg cgccgtcggc gcagcggacc gacgtctatc tcggttatga
      541 ctcgcgcaat ctgtatgcgg tgttcctcgc gttcgacgac gacccggcca gcgtgcgcgc
      601 gaatcttgcg ccgcgcgaga acatcgacaa cgacgacaac ctcggcttgg tcatcgatac
      661 gttcgacgat cagcgtacgg gttatggttt ttactcgagc ccgctcggcg tgcagtggga
      721 cggccgctgg tcggacgtaa cgcgcggcgg ttgggacacg tcgtacgagg ccgtgtggca
      781 aaccgacgcg cagctcacgg acggcggcta cgtcgtcgtc atgacgattc cgttccgcgc
      841 gatgcggttt cccgagacga gcgagcagcg gtggcgcgtg cagttcgagc gcatcattcc
      901 gcggctcagc gaggagtcgc agtggcccgc ttggtcgcaa gccatcgacg gccgcctgaa
      961 tcagacggct gtcgtgacgg gcgtgcgcga cgtttcaccg ggccgcaacg tccaactgat
     1021 cccgttcgcg ttcgtgcgca gctacgacgt gctggacggc ggccgcgcgg gcggccccgg
     1081 gttcacgaag gacaccgagg acgacttcgg cctcgacgcg aaggtcgtgc tgaaagacag
     1141 cttcgtgctc gacttcacct acaacccgga cttcagccaa gtcgagtcgg accagccgca
     1201 ggtcactgtc aacgagcgct tcgaggtgca gttccccgag cgccggccgt tcttcctcga
     1261 gaacgccgac tacttcgcgg cggagacgcc gctcctgttc acgcgccgca tcgtcgatcc
     1321 caaggtcggg ctcaaattca cgggccggca aggtccgtgg ggcatcggca cgatgctgat
     1381 caacgacgaa gcgccaggac agcgcgcccc gccgaccgat ccgctgtacg gcgaggatgc
     1441 cgacatcagc gtgctgcgcg tgtttcgcga tttcggcgac cagtcgcgcg cgggtttcat
     1501 gcacacggag cgcgagctcg gcaacaccta tagccgcgtc tcggcggccg atacctacat
     1561 caagctcacc gacaactggc tgaccgaatt gctcatggtg aacaccgaga accggctcgc
     1621 cgacggtcgt gtcatcacgg gccgcgagac gaactggcgg gtgaaccgca acagccgcaa
     1681 cttcaacgcc cactatcact acaccgcaca gagcagcaat ttcgcagtgc cgctcggcat
     1741 attgagccgc aactacacgc ccgatgccga aggtctgcac ggcttcatgg agtaccgttt
     1801 ctggccggaa gacacgtgga tcgaccgcat cgggccgcgc gtgtttttcc agcaccagga
     1861 agaccggtcg ggcacgcgcc tttactcgga gttctcgccg cagctgcaga tcgcgtgggc
     1921 cggcgcaagc ttctttgccg tcggcacgaa cgacattcgc gaacgcttgc ggccgcgcga
     1981 tttcgtgggc ctcacgacga cgcgcgatta cgcgcagacg cgctggttca cgaacatctc
     2041 gtcggacccg ttgcaaacgt tcggcttcag ctttaactac gaccaaggca cggtgatcaa
     2101 tttcgtgccg ccgctcggcg ccgagccgga gctcgcggac cgcgattaca tcgaaggatc
     2161 gattcgttgg cggccgatcg accgcctgcg catcgacacg acgcttttgt cgacgacgct
     2221 cgacgaccgc aacggccgcg gcaagatctt cgacgaccgc atactccgga cgcgcgtcaa
     2281 ctatcagttc tcgaaggaga tgtcgctgcg cgtgatcttg cagcacgaag agacgacccc
     2341 gacggcgctg tcccggctca cgaacgacga gaacttgaat ctcgatctat tgttccgcta
     2401 cgtcttgaac ccgtggtccg cgctgtacgt cggctacaac aacaacgaga gcaacttaca
     2461 gctcgtcgat acgccgcaag gaacgcagct cctgcgcagc gaagacctgg cgcgcgacgg
     2521 ccagcagttc ttcgtgaagt tctcgtatct gttgcagccg tagccaaccg cgctattcgt
     2581 agcgcagcgc gacgagcggg ttcaggttcg ccgcgcgccg cgccggcaat gcgcaggc
//
LOCUS       DATLEE010000597         4711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4419036, whole genome shotgun sequence.
ACCESSION   DATLEE010000597 DATLEE010000000
VERSION     DATLEE010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4419036"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1447
                     /locus_tag="VM692_12915"
     CDS             341..1447
                     /locus_tag="VM692_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53118.1"
                     /translation="MATAPAAEWGAPGVAPRFEVDPLWPKPLPNHWLLGSTIGVSVDS
                     RDHVWIIHRQQSLNTDTEASAGVNSPTGTCCMAAPNVIELDAAGNVVSSWGGPGQGYD
                     WPTSNHGITVDHMDNVWIGGNDVKDAHILKFSRTGQFLLQLGKPEQNQGSNDAVNFWR
                     VAKISIDAEANEAYIADGYGNKRVVVIDMATGARKRYWGAYGNRPDDTDLGNYDPAAA
                     PAQQFRNPVHCAEPSRDGFVYACDRVNDRIQVFRKDGTYVSELRVAPETRGAGSVWDI
                     AFSHDPGQTYIYLADGLNERVYIIDRKSMQILTSFGDGGRQPGQFFGVHSIAVDSQGN
                     IYTTETYEGKRVQKFVFRGLGPIPNQSQGAVWPQ"
     gene            1435..2613
                     /locus_tag="VM692_12920"
     CDS             1435..2613
                     /locus_tag="VM692_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53119.1"
                     /translation="MAAIARSAWALLLAASAAAAQPSAAPVDPVVVNFRAYRAALERN
                     DLAAAETAAAAALAASEAAQGRRTAVLALNLANVRLALGDRYDALSPAQTAHTLATSS
                     ADSDVDPRVAALTLGRAEIAADERSGSRRLVEAIAAAEADTALASEVYDAAVALGRWA
                     FDEQEYATAQQAWATAKKLAATTADPAYSRASALIGEGASIFLRNANPREVQTGSRMT
                     KISTPDAQSASDAFVTAQALLLPAAYAATPPQATLTAGQSAFAQAMAWQGALYARIET
                     LNEPLPLPPSTLGMQDIPSYDRRGECSMRASNAGPEIEYPTEALFRYGVGAVVAHFAL
                     DTDGTIRARTIAASIPPGPLAEAVEASLDQWRIEKLPSSSASCCVPPSYYAMVRFVLQ
                     "
     gene            2675..3643
                     /locus_tag="VM692_12925"
     CDS             2675..3643
                     /locus_tag="VM692_12925"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HUQ53120.1"
                     /translation="MKKTLLWLIALLPFGLAAQNRQAGPWWPSAEWGPMDQAGASNRI
                     TPEKIVAAFRLVQTGRVYELGQVYERGMPLGGTRDFALRLVPAIEPTGPNRVLYNDEF
                     LAAEVGQVGTQFDGLGHIGGEIRYADGSLHRVFYNGYTTDEMNAGTGLRELGIEHIKP
                     LLTRGVLVDLPAYKNLQRLAGGYEVTLADVRGALQRQGIAESSIMPGDAVLFRYGWAQ
                     LWSSPTEFMAAQLPGIGLEVARWLVERKVTVTGSDTSVTEVSPNPTGQTIPVHQELMM
                     KNGIFNIENMTFEELAADRAYEFLFVATPIRFEGATGSPLRPLAIR"
     gene            3842..4432
                     /locus_tag="VM692_12930"
     CDS             3842..4432
                     /locus_tag="VM692_12930"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HUQ53121.1"
                     /translation="MADQPTTTPSRLLRVAVWALFAAAFAFLYYNYSGAWGTEIGGLA
                     QSSVVLAYVIYIVLGAARGFALIPVTNLVVLAIPIFPPLPLFVLTLIGILISSASIYA
                     FAGSLRLGEYFERKHAEKTERVRTALKRNPTAIVTAWSFLPIVPTDLICYVCGVMKIS
                     FARFMTGVLVGEGAICAIYIFAGSSLFELGKRWLGA"
     gene            4437..>4711
                     /locus_tag="VM692_12935"
     CDS             4437..>4711
                     /locus_tag="VM692_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53122.1"
                     /translation="MTIRAWDRSRRWLASRAFTCVALAYLAPQAALAQPAPNGELVYG
                     EHCAMCHEQVDERIPHRTALHQLPAARIVRALDAGEMLSIAMTMNRD"
BASE COUNT          795 a         1586 c         1543 g          787 t
ORIGIN      
        1 cggccgcgcg acggatgcca gcgctgcgcc cgccaccgcc accagcagaa tcaagcgccg
       61 caccgtggcc gacctcttcg cgctctcgtg gaggcgccaa tttccgctat tatccgagcc
      121 aagaacaagc cctggtccaa cagggcacac cgctgacgga tgggcgcggg ctggatgccc
      181 gcggtagcaa cgcagggagc agttgtcggg gggaaccatg actcgtcgag ccggactcct
      241 gcttgtcatt gaaacggctg cagtgctcgc ggcctgcgac aattctccgt cgccgggcgc
      301 cgctgcgagc aatgcttcga gcgcgtcttc tgcagccgcc gtggccaccg cgccggccgc
      361 cgaatggggc gcgccaggcg tcgcgccgcg cttcgaggtc gatccgcttt ggccgaagcc
      421 gttgccgaac cactggctgc ttggctcgac gatcggcgtg tcggtcgact cgcgcgatca
      481 cgtctggatc atccaccgcc agcaatcgct gaacacggac accgaagcga gcgccggcgt
      541 gaattcgccg accggcacgt gctgcatggc ggcgccgaac gtcatcgagc tcgacgcggc
      601 cggcaatgtc gtgagctcgt ggggcggccc cggccaaggc tacgactggc cgacgtcgaa
      661 ccacggcatc accgtcgatc acatggacaa cgtttggatc ggcggcaacg acgtcaaaga
      721 cgcgcacatc ttgaagttct cgcgtacggg ccagttcctg ctgcaactcg gcaagccgga
      781 gcagaatcaa ggcagcaacg acgccgtgaa tttctggcgc gtcgcgaaga tctcgatcga
      841 cgccgaagcc aacgaggctt acatcgcgga cggctacggc aacaagcgcg tcgtcgtcat
      901 cgacatggcg acgggcgcgc gcaagcgtta ctggggcgcc tacggcaatc gcccagacga
      961 tacggatctc ggcaattacg atcccgccgc ggccccggcg cagcagttcc gcaaccccgt
     1021 gcactgcgcg gaaccctcgc gcgacggctt cgtttacgcc tgcgatcgcg tcaacgatcg
     1081 cattcaggtg tttcgcaagg acggcacgta cgtcagcgag ctgcgcgtgg cgcccgagac
     1141 gcgcggtgcg ggctcggttt gggacattgc gttctcgcac gacccgggcc aaacctacat
     1201 ttatctcgcc gacggcctca acgagcgcgt ctacatcatc gatcgcaagt cgatgcagat
     1261 tctgacgagc ttcggcgacg gcggccggca gcccggccaa ttcttcggcg tccacagcat
     1321 tgccgtcgac tcgcaaggca acatatacac gaccgagact tacgaaggta agcgcgtcca
     1381 gaaattcgtg ttccgcggct tggggccgat acccaaccag tcgcaaggcg cggtgtggcc
     1441 gcaatagctc gcagcgcgtg ggcgttgctc cttgcggcat cggccgcggc ggcgcagccg
     1501 agcgccgcgc ccgtcgatcc ggtcgtcgtg aactttcgcg cgtatcgtgc cgcgctcgag
     1561 cgcaacgatc tcgcggccgc tgagaccgcg gccgccgcgg cgctcgcggc atccgaagcg
     1621 gcacaaggcc ggcgcacggc cgtgctggcg ttgaacctcg ccaacgtgcg gctcgcgctc
     1681 ggtgaccgct atgacgcgct gtcgcccgcg cagaccgcac acacgctcgc gacttcgtcg
     1741 gctgattccg acgtggaccc gcgcgtcgca gcattgacgc tcggccgcgc ggagatcgcg
     1801 gcggacgagc gaagcggaag ccgccggctc gttgaggcga tcgcggccgc cgaagcagac
     1861 accgccctcg cgagcgaggt ctacgatgcc gccgtcgcgc tcggccgatg ggcgttcgat
     1921 gagcaggaat acgcgacggc gcagcaagcg tgggcgacgg cgaaaaaact ggccgcaacc
     1981 accgcggacc cggcgtactc gcgggcaagc gcgctgatcg gcgaaggcgc gtcgattttc
     2041 ttgcgcaacg ccaatccgcg cgaggtgcag accggcagtc gcatgacgaa gatctcgacg
     2101 ccggacgcgc agtccgcaag cgatgccttc gtgaccgcgc aagcgctgct gctgccggcc
     2161 gcgtacgccg cgacgccgcc gcaagcgacg ctgacggccg ggcaaagcgc gttcgcccaa
     2221 gccatggcgt ggcaaggcgc gctgtacgct cgcatcgaga cgttgaacga gcccttgccg
     2281 ctgccgccgt cgacgctcgg catgcaggac atcccgagct acgaccgcag aggcgagtgc
     2341 agcatgcgcg cctcaaacgc gggccccgag atcgagtatc cgaccgaagc gctgttccga
     2401 tacggtgtcg gcgcggtcgt tgcccacttc gcactggata ccgacgggac cattcgcgct
     2461 cgcacgatcg cggcgtcgat tccgccgggc ccgctggccg aagccgtcga agcgtcgctc
     2521 gaccagtggc gaatcgagaa actcccgagc tcctcggcga gctgctgtgt tccgccgtcg
     2581 tactacgcga tggtgcgctt cgtcctgcag taacctctcg gccgcgctcg ctcggcgcac
     2641 cgtgcttctt aattcccgtt caacgaggtg acacgtgaaa aagacgttgt tgtggctgat
     2701 cgcgctgctg ccgttcggat tagcggctca gaatcgccaa gcaggaccct ggtggccgag
     2761 cgccgagtgg ggtccgatgg atcaggcggg cgcctcgaat cgcatcacgc cggagaaaat
     2821 cgtcgcggcc tttcgcctcg tgcagacagg ccgcgtctac gagctcggcc aagtctacga
     2881 gcgcggcatg cccttagggg gaacgcgcga cttcgcgctg cggctcgtgc cggcgatcga
     2941 gccgacgggc ccgaatcgcg tgctctacaa cgacgagttc ctcgcggccg aggtcggcca
     3001 agtcgggacg cagttcgacg gtctcggcca catcggcggc gaaattcgct acgccgacgg
     3061 ctcgctgcac cgcgtgttct acaacggcta cacgacggac gagatgaacg ccggcacggg
     3121 cttgcgcgag ctgggcatcg agcacatcaa gccactgttg acgcgcggcg tgctcgtcga
     3181 cttgcctgct tacaagaacc tgcagcgcct cgcgggcggc tatgaggtca cgctcgccga
     3241 cgtgcggggc gcgctgcagc gccaaggcat cgccgagagc tcgatcatgc ccggagacgc
     3301 cgtgttgttt cggtacggat gggcgcaact ctggagctcg ccgaccgagt tcatggcggc
     3361 gcaattgccc ggcatcggtc tcgaggtcgc gcgttggctc gtcgagcgca aagtcaccgt
     3421 gacgggtagc gacacgtcgg tgacggaagt gtcgccgaac cctacggggc aaacgattcc
     3481 cgtgcaccaa gagctcatga tgaagaacgg catcttcaac atcgagaaca tgaccttcga
     3541 ggagctcgcg gcggatcgcg cttatgagtt cctgttcgtc gcgacgccga ttcgcttcga
     3601 gggcgcgacg ggctcgccac tgcggccgct cgcgattcgc tgagcggtcg cgccgcaatt
     3661 agtgcagcgg atcgcccgcg acgcccgcgg cgctttgcac gttggggaac acggctgcgg
     3721 catggctcgg ctccacggtt ttgctgcttt ggatcttcga acggcacgag gattgtgcgg
     3781 tagatcgcag ccgcgagacg cgtttcgcgc cgagacgcgg taaactgtcg cgctcgaacc
     3841 tatggccgat caaccgacca ccactccgtc gcgcctgctt cgcgtcgccg tttgggcgct
     3901 gttcgcggcc gcgttcgcgt tcctctatta caactactcc ggcgcttggg gcacggagat
     3961 cggcgggctc gcgcagtctt cggtcgtgct cgcgtacgtg atttacatcg tgctcggcgc
     4021 cgcgcgcggc tttgcactga tacccgtcac gaacttggtc gtgctcgcga tcccgatctt
     4081 tccgccgcta ccgctgttcg tgctgacgct gatcggcatt ttgatctcgt cggcgagcat
     4141 ctacgcgttc gcaggctcgc tgcggctcgg cgagtacttc gagcgcaagc atgcggaaaa
     4201 aaccgagcgc gtccggaccg cattgaagcg caaccccacg gcgatcgtca cggcctggag
     4261 tttcttgccg atcgtgccga cggatctcat ttgctacgtc tgcggcgtca tgaagatctc
     4321 gttcgcccgc ttcatgaccg gagtgctggt cggcgaaggc gcgatctgcg cgatctatat
     4381 cttcgccggc agcagcctgt tcgagctcgg caagcgctgg ctcggcgcct aagggcatga
     4441 ccatacgagc ttgggatcgc tcgcgtcgct ggctcgcgag ccgcgcgttc acgtgcgtcg
     4501 ctctcgccta cctcgcgcct caggccgcgc tcgcgcaacc ggcaccgaac ggcgagctcg
     4561 tgtacggcga gcattgcgcg atgtgccacg agcaagtcga cgagcgcatt ccgcatcgca
     4621 cggctctgca tcagctgccg gccgcgcgca tcgtgcgtgc gctcgacgcc ggcgagatgc
     4681 tctcgatcgc gatgacgatg aatcgcgacg a
//
LOCUS       DATLEE010000598         3871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_724685, whole genome shotgun sequence.
ACCESSION   DATLEE010000598 DATLEE010000000
VERSION     DATLEE010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724685"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..860)
                     /locus_tag="VM692_12940"
     CDS             complement(126..860)
                     /locus_tag="VM692_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HUQ53123.1"
                     /translation="MTPGYKTAAAVGLPPPDELVLQHGELVKRIAYHVVSRLPAHIEV
                     DDLIQAGMIGLLNAAQNFAPTKGANFETYAGIRIRGAMLDEARRSNWTPRSTFRNAKQ
                     VSLAIRSIENRTGRDAKAQEIAGTLGVSLEEYHRMVESAASSRILSYEQLAGDPERAS
                     MLPDAPEDGPETTLEEDQFRDAMAAAIAGLPERERLVLSLYYDQELNLREIGEVLEVS
                     ESRVCQIHGQAVLRVRARLDKWFNDE"
     gene            complement(857..1738)
                     /locus_tag="VM692_12945"
     CDS             complement(857..1738)
                     /locus_tag="VM692_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HUQ53124.1"
                     /translation="MPETYFDQAAGLRRLSAPRSVKVVAITGGKGGVGKTLTAVNLGA
                     ALAQLGRSTMLLDADFGLANVDVLLGMKARLNLEHVMSGTCALEDVILTSTSGLRVVP
                     ATSGSVSMATLSRVQHAGLIGAFSQLLEPLDVLLVDTAAGLTDGVITFSEAAQRVVVL
                     VCDEPASLTDAYGLIKVLSRRQPSCRFEVVANMVDSPVQGRELYEKLMRVCHRFLGIT
                     PAYFGYVPNDDYVRQAIRRQATVVEAFPSSPAARAFQRLARVVDEWDVPAQARGGVEF
                     FMERLVRADRECVAGVQ"
     gene            complement(1731..3008)
                     /gene="flhF"
                     /locus_tag="VM692_12950"
     CDS             complement(1731..3008)
                     /gene="flhF"
                     /locus_tag="VM692_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HUQ53125.1"
                     /translation="MNIRRYVANDMREALASIRTDLGPDAVMLSSRKLPNGVEVIAAV
                     DYDDSLLGNAAAAATAQADGASTAQLDEYEQVARRLAGVDNVAPVAAATPAPAPAPIP
                     APVAAPVADATADTSVALEIKDLRRLLETQLASLAWSDLNRQAPVRARLLRELAKLGV
                     DSTLAAELADEVPAEVNYQEAMRLVVRRFGERLPLVSWDMAEGGGVFAIVGPTGVGKT
                     TSLAKIAARFVLRHSVEQLGLVSTDTYRIGARQQLNNFARILRAPMQVAENAADLRRV
                     LDGFANKKLVLIDTAGMSQRDVRLANQFTTLKVEGHSVRTVLALSAGADRGCLAEAAK
                     VFKAANPEALIVTKLDEAATLGGVLSLSISSSLPVAYLSDGQRVPEDLHRAAPKRFWL
                     LHNALKLSANGATAPDEHELADRFGHMELAANA"
     gene            complement(3026..>3871)
                     /locus_tag="VM692_12955"
     CDS             complement(3026..>3871)
                     /locus_tag="VM692_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HUQ53126.1"
                     /translation="EGEIYVERDLAINPGGVAAQIVGVRTKDPAFGLDAYWIEPAAKE
                     HAQALGFTVVDAATVVATHLSELLKRHAYQLLGHEEVQHLLDRLAKTAPKLVENLTPG
                     ALTLGVVVKVMQELLVDRVPVRNIRTIAETLAEHGGRTQDPLVLLGHVREALGGSIVQ
                     SIYGLRNELPVITLDTSLEQLLRDGFKGGDGSPTFEPGLADRLHGALLDATQQQELKG
                     EPPVLLVPTQLRPWLARFTRHGAPNLAVLAYGEIPHSKSLRVVASVGSASAAGRAAPA
                     RAQAA"
BASE COUNT          617 a         1304 c         1307 g          643 t
ORIGIN      
        1 ccggagccga tgtttcggcc gacgggcggc ggcaacgttg gaaccggtgg agcaatacgt
       61 tcgacgtcag ccatagggac aactccgcgt tatggtcagt gctcccgcgc aaacgggcgc
      121 tcgcctcact catcgttgaa ccacttgtcg agccgggcgc gaacgcgcag cacggcttgg
      181 ccgtggatct gacaaacacg cgattcgctg acctcgagca cctcgccgat ctcgcgcaag
      241 ttgagctctt ggtcgtagta gagcgacagg accaagcgct cgcgctcggg cagacccgcg
      301 atggcagccg ccatggcgtc gcgaaattgg tcttcctcga gcgtcgtctc ggggccgtcc
      361 tcgggtgcgt cgggcagcat cgacgcccgc tcgggatcgc cggcgagctg ctcgtagctc
      421 aagatccggc tcgatgccgc gctctcgacc atgcggtggt actcctcgag gctcacgccg
      481 agcgtgccgg cgatctcttg cgccttggcg tcgcgacccg tgcgattttc gatcgagcgg
      541 atcgcgagcg agacctgctt ggcgttacga aacgtcgagc gcggcgtcca attcgagcgc
      601 cgcgcttcgt cgagcatcgc gccgcgaatt cggatgccgg cataggtctc gaaatttgcg
      661 cctttcgtcg gcgcgaaatt ctgcgcagca ttcaacaggc cgatcatgcc ggcttggatc
      721 aggtcgtcga cctcgatgtg ggccggcaac cggctcacga cgtggtaggc gatgcgcttg
      781 acgagctcgc cgtgctgcag cacgagctcg tcggggggcg gcagacctac cgccgcggcc
      841 gtcttgtatc caggcgtcat tgcacccccg ctacgcattc cctatccgcc cgaaccaacc
      901 gctccatgaa gaattcgacg ccgccgcgcg cctgcgccgg cacgtcccac tcgtcgacga
      961 cgcgtgcgag gcgctgaaaa gctcgcgccg caggactgct cggaaacgct tcgacgacgg
     1021 tcgcctggcg gcgaatggcc tggcgcacgt agtcgtcatt cggcacgtag ccgaaatagg
     1081 cgggcgtgat gccgaggaac cgatgacaaa cccgcatcag cttctcgtag agctcgcggc
     1141 cctggacggg cgaatcgacc atgttggcca cgacctcgaa ccggcagctc ggctggcgcc
     1201 gactcaaaac cttgatgagg ccataagcgt cggttagcga cgcgggctcg tcgcagacga
     1261 gcacgacgac gcgctgcgcg gcctcgctga acgtaatgac gccgtcggtc agtcccgccg
     1321 ccgtgtcgac gagcaacacg tcgagcggct cgagcagctg gctgaaggcg ccgatgaggc
     1381 cggcgtgctg aacgcggctc aacgtcgcca tgctgacgct gcccgacgtc gcgggtacga
     1441 ctcgcaagcc cgaggtcgac gtcaggatca cgtcctcgag cgcgcatgtc ccgctcatga
     1501 cgtgctcgag attcaggcgc gctttcatgc cgagcagcac gtcgacgttc gcgaggccga
     1561 agtcggcgtc gagcagcatc gtgctgcgcc cgagttgcgc cagcgccgcg ccgagattca
     1621 cggcggtcag tgtcttaccg acaccgcctt tgccgcccgt gatggccacg actttgacgc
     1681 tgcgcggagc gctgaggcgc cgtaggccgg ccgcttgatc gaaataggtc tcaggcattg
     1741 gcggctagct ccatgtggcc aaaacggtcc gcgagctcgt gctcgtcggg cgccgtagcg
     1801 ccgttggccg agagcttcag tgcgttgtgc aacaaccaaa aacgctttgg cgctgcgcga
     1861 tgcagatcct ccggaacgcg ctggccgtcg gagaggtagg caacaggcaa gctactgctg
     1921 atcgagagcg acaagacgcc gccgagcgtc gccgcttcgt cgagcttcgt gacgatcaac
     1981 gcctcggggt tggcagcctt gaataccttc gccgcttcgg caagacaccc gcgatccgcg
     2041 ccggccgaga gcgccagcac cgttcgaacc gaatgccctt cgactttcag cgtcgtaaat
     2101 tgattcgcga gccgcacgtc gcgctggctc atgccagccg tgtcgatcag cacgagcttc
     2161 ttgttggcga agccgtcgag gacgcggcgc aggtcggcgg cgttctcggc gacttgcatc
     2221 ggcgcgcgca ggatgcgcgc gaagttgttc agctgctgcc gagcaccgat tcggtaggtg
     2281 tccgtgctga cgaggccgag ctgctcgacg ctgtggcgca agacgaagcg cgcggcgatc
     2341 ttcgcgaggc tcgtcgtctt gccgacgccc gtcggcccga cgatcgcgaa cacgccgccg
     2401 ccctcggcca tgtcccagct cacgagcggc aggcgctcgc cgaaccggcg cacgacgagg
     2461 cgcatggctt cctgataatt gacctcggcc ggcacctcgt cggcgagctc cgccgccaac
     2521 gtcgagtcga cgccgagctt cgcgagctcg cgcagcaggc gcgcgcgcac gggtgcttgg
     2581 cgattcagat cgctccaggc caggctcgcg agctgcgttt cgagcaagcg gcgcaggtct
     2641 ttgatctcga gagcgacgga cgtatcggcc gtcgcgtcgg caaccggcgc ggcgaccgga
     2701 gcaggaatcg gggcgggggc gggcgccgga gtcgcggccg cgaccggggc gacgttgtcc
     2761 acgcccgcga gccgccgcgc gacctgctcg tactcgtcga gctgcgccgt gcttgcaccg
     2821 tccgcttgcg ccgtggccgc agcggcggcg ttgccgagca ggctgtcgtc gtagtcgacg
     2881 gccgcgatga cctcgacgcc attcggcaac ttgcggctcg acagcatcac ggcgtcgggg
     2941 cctagatccg tgcggatcga ggccagcgcc tcgcgcatgt cgttggcaac gtatcgtctg
     3001 atattcactc gttctccttc gcgatctagg cagcctgggc acgcgcgggc gcggcgcggc
     3061 cggcagcgct cgcgctaccg acgctggcca cgactcgcag cgatttgctg tgcggaatct
     3121 cgccgtaggc gagcaccgcg agattcggcg caccgtggcg cgtgaagcgc gcgagccacg
     3181 gccgcaattg cgtcggcacg agcagcacgg gcggctcgcc tttcagctct tgctgctgcg
     3241 tcgcgtcgag cagcgcgccg tgcaggcggt cggcgaggcc cggctcgaac gtcgggctgc
     3301 cgtcgccgcc cttgaagccg tcgcgcaaca attgctcgag cgaagtgtcg agcgtgatga
     3361 cgggcagctc gttgcgcagg ccgtaaatcg actgcacgat cgagccgccg agcgcctcgc
     3421 gcacgtgacc cagcagcacg agcgggtcct gcgtgcgccc gccgtgctcg gcgagagttt
     3481 ctgcgatcgt gcggatgttg cgcaccggca cgcggtcgac gagcagctcc tgcatgacct
     3541 tgacgacgac gccgagcgtc aacgcgcccg gagtcaggtt ctcgacgagc ttcggcgccg
     3601 tcttggcgag gcggtcgagc agatgctgca cttcctcgtg gccgagcagc tgatacgcgt
     3661 ggcgcttcag gagctcggag aggtgcgtcg cgacgaccgt cgccgcgtcg acgaccgtga
     3721 agccgagtgc ctgcgcatgc tcctttgcgg ccggctcgat ccagtacgcg tcgagaccga
     3781 acgccgggtc cttcgtgcgc acgccgacga tttgcgccgc cacgccgcca ggattgatcg
     3841 cgagatcgcg ctcgacgtaa atctcgcctt c
//
LOCUS       DATLEE010000599         2405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7071137, whole genome shotgun sequence.
ACCESSION   DATLEE010000599 DATLEE010000000
VERSION     DATLEE010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7071137"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VM692_12960"
     CDS             <1..1193
                     /locus_tag="VM692_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53127.1"
                     /translation="QRACFSLTCLQADHASLCVQAASTLETCLAFLQRLEAARRHSSS
                     AGVALLLGETLTGLARKDVPPQAKERYLERARAAFKDVVRLQPLSASGYLGLAEVAGT
                     GDERVEWLRGAVQAEFQPAHMELLSRALSDVGGNAAELEAARVLDDAYMLESSETQKW
                     RYAASSLQKHTVALERYPLAENGRSVDNVVLRFEDDIDYVLLQRMLLEPESYLAYLSN
                     ALATMCEKSVAAIVTLDECMAGLELSVVTAEGSPSAGERRLLAEAVLIGMRTIAGESL
                     PRSPITQKKFRDWIDRLLTTTLEPVQVSVDLLEARADYTANLLDRAQTLLAAIELVPN
                     QGDLRLKLGATYVSLWFWPEALEELRVARYFLPEDEHERVDRLVATADKGYQARFSPP
                     SDAN"
     gene            1298..1894
                     /locus_tag="VM692_12965"
     CDS             1298..1894
                     /locus_tag="VM692_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53128.1"
                     /translation="MRHVRYGLAAAALIGAPLAMAQLEVGGSALDNCSKIDALRKAGN
                     YTEARDKAQACLDALEQEVTGSVGKLFSTNVAGWARANLQQDKVLGFTNVTATYKKGE
                     HEATVALTGTGGGGGGGLGGLLGGFARAGLAGSGQQVRVGGLPASVQPDGTITVTLED
                     GSFLTFSSSSFTSQETALAGLGDLVNGFPVAEINKALQ"
     gene            complement(1940..>2405)
                     /locus_tag="VM692_12970"
     CDS             complement(1940..>2405)
                     /locus_tag="VM692_12970"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ53129.1"
                     /translation="VLAFKADVTRVATMMFSRDLSAKVYAESGVPDAYHALSHHQDDP
                     VRMERYAKLNAYHVSIVGYFLDKLRNTPEGGSNLLDNSLVMFGSPMGNSNMHDHNDLP
                     ILVAGTAAGRYQTNRHLEQPKGTPIANFFMTLAAKEDIELPSFGDSTGTMDV"
BASE COUNT          407 a          742 c          829 g          427 t
ORIGIN      
        1 cgcagcgtgc gtgcttcagc ctgacgtgcc tgcaagcgga ccatgcatcc ctgtgcgtgc
       61 aggccgcttc gacgctcgag acttgcctcg cgtttctgca gcggctcgag gcggcgcggc
      121 gacactcgtc gtccgccggc gtggcgctgc tgctgggcga aacgctgacc ggcttagcgc
      181 gtaaagacgt acctccgcaa gcgaaagagc gttacctcga gcgggcgcgt gccgccttca
      241 aggacgtcgt gcgcttgcag ccgctgagcg cgtcgggcta cttggggctc gccgaggtcg
      301 cgggcacggg cgacgagcga gtcgaatggc tgcggggcgc cgtgcaagcg gagttccagc
      361 cagcgcacat ggagttgctg tcacgcgcgt tgtccgacgt cggcggtaat gctgcggagc
      421 tcgaagccgc ccgcgtgtta gatgacgcgt atatgctcga gtcgagcgag acgcagaagt
      481 ggcgctacgc ggcgtcgtcg ttgcagaagc acaccgtcgc gttggagcgt tacccgttgg
      541 cggaaaacgg caggtccgtc gacaacgtcg tgctgcgctt cgaagacgac atcgactacg
      601 tgctgctgca gcgcatgttg ctcgagcccg aatcgtatct tgcgtacctg tccaatgctc
      661 tggcgacgat gtgcgagaag agcgtcgcag cgatcgtgac gctcgacgag tgcatggccg
      721 gcctcgagct gtccgtggtt acggcggagg ggtcgccgtc cgccggtgag cggcgcttgc
      781 tcgccgaagc cgtgctgatt gggatgcgaa ccatcgctgg agagtcgctg cctcgctcgc
      841 cgatcacgca gaaaaaattc cgcgactgga tcgatcggtt gttgacgacg acgctcgagc
      901 cggtccaggt ctcggtcgac ttgctcgagg cgcgggcgga ttacacggcc aacctgctcg
      961 acagggcgca gacgttgctc gccgcgatcg agctcgtccc gaatcagggc gatcttcgct
     1021 tgaagctcgg cgccacgtac gtgagtctct ggttttggcc ggaagcgctc gaggagctac
     1081 gcgtcgcccg atatttcttg cccgaggatg agcacgagcg cgtggaccgg ctcgtcgcaa
     1141 cggctgacaa gggctatcag gcgcgcttct cgccgccgag cgacgccaac taacgcgctg
     1201 gacccgctcc gcatccgcgg attaatctct gcttgcgtgg ccgcgacaca tgatgtgtct
     1261 ggccgcgcca aggacgccaa caaccatgga ggggaaaatg agacacgtac gatacggcct
     1321 cgcggccgcc gctttgatcg gcgcaccgct cgcgatggca caactcgagg tcggcggcag
     1381 cgccctcgac aactgctcga agatcgacgc gctgcgcaaa gccggcaact acaccgaggc
     1441 acgtgacaag gcgcaggctt gcctcgacgc gctcgagcaa gaagtcacgg gctcggtcgg
     1501 caaattgttc tccacgaacg tcgccggctg ggcacgcgcg aacctacagc aagacaaggt
     1561 tctcgggttt accaacgtga ctgcgactta taagaagggc gagcacgagg ccacggtggc
     1621 gctgacgggc acgggcggcg gcggtggcgg cgggctcggc gggttgctag gcgggttcgc
     1681 gcgtgccggt ttggccggat cgggtcaaca ggtccgcgtg ggcggcctgc cggcttcagt
     1741 gcagccggat gggacgatca cggtcacgct cgaggacggc tcgttcctca cgttctcgtc
     1801 gtcgagcttc acgtcgcagg aaacagcgct cgccgggttg ggagatctcg tcaacggatt
     1861 ccccgtcgcc gagatcaaca aggcgctgca gtagcacgca tcgcgtgccg gcgccggcct
     1921 cggcgccggg cgcgagacgt tagacgtcca tcgtgcccgt gctgtcgccg aagctcggca
     1981 gctcgatgtc ttccttggcc gccaacgtca tgaagaaatt ggcaatcggc gtgcccttcg
     2041 gctgctcgag gtgccgattc gtctggtagc ggcccgcagc cgtgccggcg acgaggatcg
     2101 gcagatcgtt gtgatcgtgc atgttcgagt tgcccatcgg gctgccgaac atgacgagcg
     2161 agttgtcgag caggttcgag ccgccttccg gcgtgttgcg cagcttgtcg aggaagtacc
     2221 cgacgatcga cacgtgatac gcgttgagct tggcgtagcg ctccatgcgg accgggtcgt
     2281 cttggtggtg cgacaacgcg tgataggcat cgggtacgcc gctctcggcg taaaccttcg
     2341 cgctcaagtc gcgcgagaac atcatcgtcg cgacgcgcgt gacgtcggcc ttgaacgcga
     2401 ggact
//
LOCUS       DATLEE010000600         3469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10800780, whole genome shotgun sequence.
ACCESSION   DATLEE010000600 DATLEE010000000
VERSION     DATLEE010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10800780"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1711
                     /locus_tag="VM692_12975"
     CDS             179..1711
                     /locus_tag="VM692_12975"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HUQ53130.1"
                     /translation="MTLRAGCRASAAVLAALLSFTASAADLFDFGLATSGARIDAVAV
                     AARSADAPTVLVIGGLHGEDETTAAVRRAVSDFERLRARDRDVNLLAVPLANPDGAPL
                     EFPPSGVAYREHSESHALWRWIGTQAPDLVLVADSADFGLAAALGEHAAADMGRIPAR
                     RWSGPEELRALRAARVPKSDARRELESRRLRTPRQVADELAMRYGRDFDQPWYIAAVA
                     LLAQLELGHLDDVRRLAEPYVDGTKDSLAQPNSLVMAGHIVFTELARRTGDARYVAAV
                     RKVADLGFDASGAMKESMPYHNEFSDSVFMGTVIAAQAGALTGERKYFDMADRHLRFM
                     EALDLRPDGLYRHQPATDAAWGRGNGFAAIGLALTLSELPRDHEGYAHALSSYRSLMA
                     ALAPQQNRDGLWRNVVDHPGAYAELSATAMIGFAMLRGLRSGWLAGDDYERAVERAWI
                     AVSSRSSAAGTFIDVCESTARMTSLEQYLRRTAILGEDPRAGAMVMLFATELGKPRAA
                     PR"
     gene            1941..2651
                     /locus_tag="VM692_12980"
     CDS             1941..2651
                     /locus_tag="VM692_12980"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUQ53131.1"
                     /translation="MVLLALAGAPALAQAPAQEAPKNLQVLPKDWTRAQVVNVMQNIN
                     AALGVGCDYCHVQNQGAPPDFAADDKPEKKAARAMMKLTGELNTDLVAALERPAAEVT
                     RVGCITCHRGVPEPKQLSEILAKTSADKGFPAAKAQYDELKAKYYGAQAYDFSDAGLI
                     ATAQPLVQGRTDEAIQFLRMNLDLFPRSARTYVALSQAQVAKQDRAGAIASLEKALEI
                     EPNNAAAKRSLDQLRAQR"
     gene            2794..>3469
                     /locus_tag="VM692_12985"
     CDS             2794..>3469
                     /locus_tag="VM692_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HUQ53132.1"
                     /translation="MAKNLEEAIRQAGGPVNLLWESKAPPAVVPRVVQEFTNWRDEQL
                     AWRRTAVIFDQSHHMADLNINGPDALKLISKLAVNSVAKFPVDMAKQFVAVNYDGYII
                     GDNILFHLEEDEYQAVGIPPSINWLHYHATTGDYDVRVWRDDNSLYRKGDPVLYRYQV
                     QGPGAMNVIREATGEEPPKLRFFHMTRLKIAGKQVRALRHGMAGEPGLEMWGPWADND
                     AVHAALL"
BASE COUNT          591 a         1152 c         1160 g          566 t
ORIGIN      
        1 aacgccgtcg caaacgccgg ggctttgagc cacgacgcgc caacggtggc cgactaccgg
       61 ctcatcctgc gcggcagcaa agggccgctc gggaatctcg agcccaagga gctctacgcg
      121 cgcgcctgcg agttgggatg gccgagcgcg tgcacaccgc gatgacggga cgccgcgaat
      181 gacgttgcgg gccggctgcc gtgctagcgc ggctgtgctc gcggcgctgt tgtccttcac
      241 ggcgtccgcc gcggacctct tcgatttcgg tctcgcaacg agcggcgctc gcatcgatgc
      301 cgtcgccgtc gccgcacgct cggccgatgc accgaccgtc ctcgtgatcg gcggcttgca
      361 cggcgaggac gagacgacgg ccgcggtacg tcgcgcagtg tcggacttcg agcgcctgcg
      421 cgcgcgcgat cgagacgtga atctgctcgc cgtgccactc gcgaatcccg acggcgcgcc
      481 gctcgagttt ccgccgtcgg gtgttgcgta tcgcgagcac tccgagtcgc acgcgctgtg
      541 gcgctggatc ggcacgcaag cgcccgacct cgtgttggtc gcggacagcg cagacttcgg
      601 cttggccgcc gcgctcggcg agcacgcggc cgccgacatg ggtcgcattc cggcgcgtcg
      661 ctggagcggg cccgaggagc ttcgcgcgct gcgcgctgcg cgcgtgccga agtccgacgc
      721 gcggcgcgag ctcgagagtc gtcgcctgcg aacaccccgg caagtggctg acgagctcgc
      781 gatgcgctac ggccgcgatt tcgaccagcc ctggtacata gcggccgtcg cgttgctcgc
      841 gcagctcgag ctcggtcacc tcgacgacgt ccgtcgcctg gccgagccgt acgtagacgg
      901 cacgaaggac agtctcgcgc agccgaactc gctcgtgatg gccgggcaca tcgtgtttac
      961 ggagcttgcg cggcgcacgg gcgatgcgcg ttatgtcgcg gccgtgcgca aagtggcgga
     1021 cttgggcttc gatgcttccg gtgcgatgaa ggagtcgatg ccgtatcaca acgagttcag
     1081 cgactcggtc ttcatgggca ccgtcatcgc cgcgcaggcg ggcgccctca cgggcgagcg
     1141 gaagtacttc gacatggccg accgccatct gcgtttcatg gaggcgctcg atcttcggcc
     1201 cgacggcttg taccgtcatc aaccggccac cgacgcggct tggggccgcg gcaacggctt
     1261 cgcggccatc ggtctcgcgc tgacgttgtc ggagctgccg cgcgatcacg aaggctacgc
     1321 gcatgcgctg tcgagctatc gctccctgat ggccgcgctc gccccgcagc agaatcgcga
     1381 cggcctctgg cgcaacgtcg tcgatcatcc cggcgcctat gcggagctct cggccactgc
     1441 gatgatcggc tttgcgatgc tgcgcggctt gcgcagcggt tggcttgcag gcgacgacta
     1501 cgagcgcgcc gtcgagcgcg catggatcgc ggtgagctca cgctcgtcgg cggcgggcac
     1561 gttcatcgac gtctgcgagt ccacggcacg gatgacgtcg ctcgagcagt acctgcggcg
     1621 cacggccatc ttgggcgagg acccgcgcgc cggtgcgatg gtcatgctat tcgcgacgga
     1681 gctcggcaag ccgcgcgccg cgccgcgctg agctgtcgcg ctgcaccgct actcggggcg
     1741 cgcacgggcg aacgttccaa ggtggcagcg acggcaatag cgtcggcgtc gcctgagact
     1801 cgttctcgtt tccgtcgctc gttgaaaact gctcgagaag agcggagcat cgcactccgt
     1861 ctgcgcacga aaccaacaat gaattcgagg gggttatgac gatgagccgt cccgcttcac
     1921 tgctttccgg atcgctgtct gtggttctcc tggcactcgc gggggctccc gcgcttgcgc
     1981 aagcgcctgc ccaggaagca ccgaaaaacc ttcaggtcct accgaaggat tggacgcgcg
     2041 cccaagtcgt caacgtcatg caaaacatca atgcggcgct gggcgtgggc tgcgactact
     2101 gccacgtcca aaaccaaggc gctccgcccg atttcgccgc cgacgacaag ccggagaaga
     2161 aggcggctcg cgcgatgatg aagctgaccg gcgagctcaa caccgatctc gtggcagcgc
     2221 tcgagcggcc ggcggccgaa gtgactcgcg tcgggtgcat cacgtgccat cgcggcgtgc
     2281 cggaaccgaa gcagctctcc gagatcctcg cgaagacgtc cgccgataag ggcttcccgg
     2341 ccgccaaagc gcaatacgac gagctgaagg ccaaatacta cggagcgcaa gcttacgact
     2401 tcagcgatgc cggcctgatc gcgacggcgc agccgctcgt gcaaggccgc acggacgagg
     2461 cgattcaatt cctgcgcatg aacctcgacc tgttcccgcg atcggcgcga acctatgttg
     2521 cgctgtcgca agcgcaagtc gcgaagcagg atcgggcggg cgcgatcgcg agcctcgaga
     2581 aagccctcga gatcgagccg aacaacgcgg cggcgaagcg ctcgctggac cagctgcgcg
     2641 cgcaacgcta gagcagcgcg ctcgctttga cgcggtatcc gcggcgctcg cccactaggg
     2701 cgagcgcctt ctcgcggaga ctcatgaagt agaatttcac cgtgtcgcga cggagctcgc
     2761 gtcaccgggt cattcgttaa tggagtggag aacgtggcga agaatttaga agaggcgatc
     2821 cggcaagccg gcggccccgt caaccttctc tgggaatcga aagccccgcc ggccgtcgtg
     2881 ccgcgcgtcg tgcaggagtt caccaactgg cgcgacgagc aactggcctg gcgccgcacg
     2941 gcggtcatct tcgatcagtc gcatcacatg gcagacctca acatcaacgg tcccgatgcg
     3001 ctcaagctca tcagcaagct cgccgtcaac agcgttgcga agttccccgt cgacatggcg
     3061 aagcagttcg tcgcggtgaa ctacgacggt tacatcatcg gcgacaacat cctgtttcat
     3121 ctagaggaag acgagtacca agccgtcggc atcccgccgt cgattaactg gctgcactat
     3181 cacgccacga cgggcgacta cgacgttcgc gtttggcgcg acgacaactc gctctatcgc
     3241 aagggcgacc cggttctcta ccgctatcag gtgcaggggc cgggcgccat gaacgtcatt
     3301 cgcgaggcga cgggggagga gccgccgaag ctgcgcttct ttcatatgac gcgcttgaag
     3361 atcgcgggta agcaagtgag ggccttgcgc catggcatgg ccggcgagcc gggcctcgag
     3421 atgtgggggc cgtgggccga caacgacgcc gtccatgcgg cgctgctcg
//
LOCUS       DATLEE010000601        13478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_726255, whole genome shotgun sequence.
ACCESSION   DATLEE010000601 DATLEE010000000
VERSION     DATLEE010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 13478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13478
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726255"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VM692_12990"
     CDS             <1..214
                     /locus_tag="VM692_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53133.1"
                     /translation="PYSAQQQRAFAVALLLGTASSGGIKLELGPGDVEEPAPLAPLFM
                     AWALSNETTFRRTAGGQGWTWRRLGA"
     gene            204..1001
                     /locus_tag="VM692_12995"
     CDS             204..1001
                     /locus_tag="VM692_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUQ53134.1"
                     /translation="MGRSWRLFATALAFALFGIAAVVVNVLYFVPVRLLTRGAARRRL
                     ARSGIRVSYKAFLQLLALLGVVRFDLDRAGLAALGGAGTVIVANHPTLLDVIVLLAYV
                     EQAGCVVKKTLWRNPFVSVAITAAGYIPSNDPEHLLRDCDEALKRNESLIVFPEATRT
                     VPGEPLRLHRGAAAIALESGAELQIVHLSCEPVLLPKGAAWYRIPDVRPCLAARVGAS
                     LRARDFQADGANRSVAARHLTLALQHELSRGIRLDARAGTGAQTTTH"
     gene            961..1221
                     /locus_tag="VM692_13000"
     CDS             961..1221
                     /locus_tag="VM692_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017024907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HUQ53135.1"
                     /translation="MQELERELKQLLIEVLNLEDISVEQIDSEAPLFGEGLGLDSIDA
                     LELGVAIRKKYDVQMDADSETTRKHFASIANLARFISSSHAV"
     gene            1211..1471
                     /locus_tag="VM692_13005"
     CDS             1211..1471
                     /locus_tag="VM692_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HUQ53136.1"
                     /translation="MRFSVLSREQIFSELKRELCALFELTPEQITPKARLYDDLDLDS
                     IDAVDLAVRLQNLTKKRIKPEQFKAVRTVEDVVDAVEQMLQA"
     gene            1499..2089
                     /locus_tag="VM692_13010"
     CDS             1499..2089
                     /locus_tag="VM692_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53137.1"
                     /translation="MRLFPTVGTLVSVAVVLALAAYPLVVYTTIGRFGPTGVAALLAA
                     VCLARLVILKLRAPASFGGGYLTAVCVGGLLLAAASFWLDSTGAVLYYPVLVNAALLV
                     VFAASLVSPPSAIERIARLRDPDLPPAAVVYTRRVTIVWAVFFALNGAAALYTALCTT
                     LETWALYNGLIAYVLIGALFAAELAIRTAVKSRLQR"
     gene            2086..3426
                     /locus_tag="VM692_13015"
     CDS             2086..3426
                     /locus_tag="VM692_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HUQ53138.1"
                     /translation="MTPSRLAALVASATDHLVCFERDGTERSWRELVAAAASVAAQLR
                     SVDGERWALNLGGGFEAAAALLGCWAAGRTPVIAPATLLTTLDSAALDGVIELAGDAT
                     PAPARIVWQDLTPTRDPLPTISARAALVLYTSGSTGVPKAARRRLFNIESELGALEAV
                     FGQRLGRVPVFATASHRHVYGLLFRTLWPLLERRPFATFDYEYPEQLLGTASRGNVLI
                     SSPAMLKRIGHLPAGSGQWRAIFSSGGFLPPEAARDVERVLGVEPVEVLGSTETSGVA
                     WRTPSTSGFELLPSVEVRAAADDLLEVRSPFAGADGWQSIGDRVAFRGDGRFDLLGRA
                     DRIAKIEDKRVSLSEIERRLLEHAYVKDAVAVALEDDARQYVGAVIELTQIGREAIAA
                     HGKRAVGSALRTSLRGRIDAVALPRAFRYPSAMPVDAQGKRQIAALARLFGRTP"
     gene            3423..3764
                     /locus_tag="VM692_13020"
     CDS             3423..3764
                     /locus_tag="VM692_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53139.1"
                     /translation="MTTEPIVVSQRVAPPDAELHLTIPADLDYFRGHFDGAPVVPGVV
                     QVKWAVEAARRHLGAAGEVSGMENLKFQKVLVPGAAATLTLKWVAADRKLYFSYHRDA
                     DRFSSGRLLFR"
     gene            3770..5428
                     /locus_tag="VM692_13025"
     CDS             3770..5428
                     /locus_tag="VM692_13025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010633044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ53140.1"
                     /translation="MKVAIVVPVYNHGEPLRATAARLAEFGLPVLIVDDGSDEATKCA
                     IAEVAARYRYEAVTLPRNGGKGQAVVAGLRAAAAHGYTHAVQVDADGQHDLADLPKLL
                     AAAKENPAALICGEPRFDASVPKARLYGRKLTAFWVAIETVSLRMPDTMCGYRVYPLA
                     ATLAIIDSVTIGRRMDFDIEIVVRLYWHNVPIVKVPTAVIYPEGGTSNFRTLADNALI
                     SKLHTKLTFGMLWRLPMLVTRRVGHALHWADLGERGGVAGMHVLFGVYRFFGRRAFTA
                     LLYPVVGYFSVAARSPRRASREYLAAIRARLVELGRPVPPQLSVFHHVLEFGNGVLDK
                     VAMWADAMPNRGITQEDFEMLERFRKESRGGALFIGSHLGNLEMLRAVGDVQKMTVNA
                     LVFTRHSPKFNRVLAAVSPKTLERMIQVDSLGPEAVIMLRDKLASGEHVAIVGDRVSV
                     RHKERSVRAPFMGRPAPFPEGPFILASLLECPVYLLFCLKIEGRYRVFMEPFADPLVL
                     PRGGRQQALERAVERYAARLEAHCLLAPTQWFNFFDFWGQAGGH"
     gene            5465..5908
                     /locus_tag="VM692_13030"
     CDS             5465..5908
                     /locus_tag="VM692_13030"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HUQ53141.1"
                     /translation="MLQSEIELEVPFHDCDPMGVTWHGCYVAYLELARSALLNRIGYN
                     FRQMTASGFAWPVVDLKVKYVKTTTFGQRLVVSAALEEYENRLKIAYTIADRQSGDIV
                     TKASTTQVAVDQARGEMCFVSPQTFIDKVREALTAAPRAGGQRVL"
     gene            5905..7083
                     /locus_tag="VM692_13035"
     CDS             5905..7083
                     /locus_tag="VM692_13035"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005499796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HUQ53142.1"
                     /translation="MKLYLNDMGVIGPLGAGKREVLSNLLAGDTGGMVPYSELLTGRA
                     TTIGRVRREPEAVPRELAELDCRNNRLLQHALDEIRVSASALGAAVGPGRVAVVLGTS
                     TSGIASGEEAAQALARDGRMPPHFHYKQQELGTAAEFAARYLGLDGLRYTISTACSSS
                     AKAFASAARLFAADLCDAAIVGGVDSLCLLTLNGFDALESLAAERCNPFSANRRGINI
                     GEGACVFLVSRDESAIRLYGVGESSDGYHISAPDPSGRGAEAAIRAALADAGLAAGDV
                     GYVNLHGTATPKNDQMESEVMARVFGLDVPCSSTKSLIGHALGAAGAQELGLCWLLLS
                     DANGGRRLPGHVWDGVRDASLPAINLVGTDARFERDVFVSNSFAFGGSNAAIAIGRAR
                     "
     gene            7080..7541
                     /locus_tag="VM692_13040"
     CDS             7080..7541
                     /locus_tag="VM692_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxylacyl-ACP dehydratase"
                     /protein_id="HUQ53143.1"
                     /translation="MNSAAIPIGELLPHGPEMTLIERLVEYSAQRSVATVTITPRSRF
                     VEPGGVPSWVGIEYMAQTIAAHAGYEARLRGEPPAIGFLLGTRAYRSDVAAFALGAKL
                     TITVEPLVTDAKLAAFQCSIATQRVVATAVVNTYQPAAGELDAMRRGTVAP"
     gene            7538..8263
                     /locus_tag="VM692_13045"
     CDS             7538..8263
                     /locus_tag="VM692_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008218191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HUQ53144.1"
                     /translation="MTRSVLVTGASKGLGAAIALALGRQGFTIAVHYRSDAEGAARTA
                     EGIRGAGGECRVLQFDVTDRAACRTALEADIAAHGAYYGVVNNAGVHRDNAFPLLTER
                     DWDEVIRTDLDGFYNVLQPCVMPMIQARRGGRIVTLSSVSGVLGNRGQVNYSAAKAGV
                     IGATKALALELAKRKITVNCVAPGLIDNVELGDEDKERILEMIPVRRLGTPEEVAALV
                     AYLMSDLAAYVTRQVISIDGGLS"
     gene            8260..9489
                     /locus_tag="VM692_13050"
     CDS             8260..9489
                     /locus_tag="VM692_13050"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010439211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HUQ53145.1"
                     /translation="MTARVAVTGMAGLTSLGQDYDAVIGAMDAGRSGIRFMEEWSRYD
                     GLNTHLGGPIDDFALPEHYPRKKIRSMGRVASLAVVATERALGAAALASSDIVTNGEC
                     GVAYGSSSGSPDAIADFGAMLNEHSTRMINSTSYIRMMSHTAAVNTSLFFNIRGRLVS
                     TSTACTSGSQAIGYAYEAVKHGAQRVMVAGGAEELSATQAAVFDTLFATSCKNDAPTT
                     TPRPWDRDRDGLVIGEGAATLILEDWDSAVARGARIFAEIVGFGTNCDARHVTQPTSE
                     TMEEAMRRALRDSGLDAAAIGYVSGHGTATELGDIAESHATLAVFGEGTPISCMKSYF
                     GHTLGACGAQEAWLAIEMMNRDHFAPTLNLDHVDPRCASLDYIVGAPRTLSTEYVMSN
                     NFAFGGINTSLIFRRVS"
     gene            9486..10238
                     /locus_tag="VM692_13055"
     CDS             9486..10238
                     /locus_tag="VM692_13055"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HUQ53146.1"
                     /translation="MTGDVRDASKSTGVVHVYYADRRALPAAAVEALVTAEDRQRVTG
                     TMHPRRTAEYLASRALLRHSLARHTARSAAELRIETSADGKPSCVGGPEVSVSHSGDI
                     VMCALAAAGAVGVDVETAGPRSVAAVAERFFTAAEARWVAAQPEPRFRQLWVLKEAYL
                     KALGVGLAGGLSSLECRVELPSIVARVAGAPLRGLPGGEGGAPQLALLQGKGCSVGVA
                     SLAATPLELVLEHVALDGSADAVGPLALLART"
     gene            complement(10286..10885)
                     /locus_tag="VM692_13060"
     CDS             complement(10286..10885)
                     /locus_tag="VM692_13060"
                     /inference="COORDINATES: protein motif:HMM:NF043005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCE4755 family polysaccharide monooxygenase-like
                     protein"
                     /protein_id="HUQ53147.1"
                     /translation="MKKWLLAFPVAGIATLGVAPAVYAHFKLESPAAWLEQDERGDPQ
                     KLAPCGGTLADPGEPTTAMTAVTGGSKLKVVVNETIFHPGHFRISLARKRNLLPLDPA
                     VTMHETERGLRSQSAFIMTTPMPPILVDNLWPHTEKPEKNWETEITIPNITCEGCQLQ
                     VIQVMLEHPGVREGGFSYHHCAVLNITADPAKPNEDANW"
     gene            11043..11918
                     /locus_tag="VM692_13065"
     CDS             11043..11918
                     /locus_tag="VM692_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HUQ53148.1"
                     /translation="MTAKTIKRAHPAMRDDIADLTTRRPVPGQDVEQIDPFLFLNHHG
                     PQVYAPRNRGLPFGPHPHRGFETVTFILTGELAHRDSAGHESIIKEGGIQWMTAGSGL
                     VHAEISPEEFMRTGGPLEILQLWVNLPPRLKMTQPRYIGLQRDEIPAVPTPDGKGVVN
                     LVSGEYLGRKGAMQPLLDIFMSTVELEAGARVEFSGVAQRNVFLYVVRGSIKVAGEPA
                     PHWHLLELSDEGDTVAVEADTEAVLLFGHAEPIGAPVVAYGPFVMNTRDEIMQAIQDY
                     QAGKFNAIPAGRATA"
     gene            11915..12529
                     /locus_tag="VM692_13070"
     CDS             11915..12529
                     /locus_tag="VM692_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HUQ53149.1"
                     /translation="MKPRKPLRNVIFDFGGVLVTWRPQEIIDSFYEEPALREALRTHA
                     FQHDDWLDMDRGTLDEAAVALRCAARMARPEAELKALFDHVRAALQPIDATVAFLREL
                     RERADLKLYGLSNMSATIFAYLAGRHDFFELFDGIVVSAHIKLLKPEPEIYVHLRDRF
                     DLDFAESVFIDDMARNVESARAVGLPSIQFLSTDQVRRELAALL"
     gene            12587..13351
                     /locus_tag="VM692_13075"
     CDS             12587..13351
                     /locus_tag="VM692_13075"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HUQ53150.1"
                     /translation="MRAKSLLLGIAVCLLGGLASAQFGRRAPEPGGAPPEAEFHMARL
                     AYYARGCAGSRGYCNPWWAIDYPYAEAHFLPAVERMTRIEVAPDSRHITIDDEYLFDY
                     PWLFLQQPGQGNWHPQGEELERLREYLVRGGFLVVDDFHNEYEWQAVRDAMADLLPGR
                     PIVDIPDDDALHHILFDLDGRTQIPGERHLYRGMEGPPHWRGIYDDRGRLMVALNHNS
                     DMGDAWEHADDAHYPTPMTATAYRFGVNYVIYAMTH"
     gene            13353..>13478
                     /locus_tag="VM692_13080"
     CDS             13353..>13478
                     /locus_tag="VM692_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HUQ53151.1"
                     /translation="MPAKLYLDDLAVGRKFVSSETAVTLEACKAFAVQFDPQPFHL"
BASE COUNT         2099 a         4466 c         4571 g         2342 t
ORIGIN      
        1 cccgtacagc gcgcaacaac agcgtgcttt tgcggttgcg ttgctgctcg gcacggcatc
       61 gagcggcggg atcaagctgg agctggggcc gggcgacgtc gaggagccgg caccgcttgc
      121 gccgttgttc atggcttggg cgctgtcgaa cgagacgacg ttcaggcgca cggccggtgg
      181 ccaggggtgg acctggaggc ggcttggggc gtagctggcg cttgtttgcc accgcgctgg
      241 cgttcgcact tttcggcatc gccgccgtcg tcgtcaacgt gctgtacttc gtgccggtgc
      301 gcttgttgac gcgcggagcg gctcgccggc gtcttgcgcg ctccggcatt cgcgtgagct
      361 acaaggcctt cttgcagctg ctggcactgc tcggtgtcgt tcggttcgat ctcgatcgcg
      421 caggcctcgc ggcgctcggc ggggcgggca ccgtgatcgt cgccaatcac ccgacgctgc
      481 tcgacgtcat cgtgttgctc gcgtacgtgg agcaagccgg ctgcgtcgtc aaaaagactc
      541 tgtggcgcaa tccgttcgtg tccgtggcga tcaccgccgc aggctacatt cccagcaacg
      601 atccggagca cctgctgcgc gattgcgacg aggcgctcaa acgcaacgag agcctgatcg
      661 tattccccga agcgacgcgc acggtgcccg gtgaaccgct gcgcctgcat cgcggcgcgg
      721 cggcgatcgc cctcgagtcc ggcgcagagc tccaaatcgt gcatttgagc tgcgagcccg
      781 tgctgctgcc gaagggcgcg gcgtggtatc gcattccgga cgtgcgtcct tgccttgcgg
      841 cgagggtggg tgctagctta cgcgcgcgcg atttccaggc ggacggcgcc aaccgtagcg
      901 tcgccgcaag acatctcacg ctcgcgctgc agcacgagct atcgaggggg attcgactag
      961 atgcaagagc tggaacggga gctcaaacaa ctactcattg aagtcttgaa cctcgaggac
     1021 atcagcgtcg agcaaataga cagcgaggca cctctattcg gtgaaggatt gggactcgat
     1081 tccatcgacg ccctggagct cggcgtcgcg atacggaaga aatacgacgt gcagatggat
     1141 gccgattccg agactacccg caagcatttc gcttcgatcg ccaacctggc ccgcttcatc
     1201 agctcgagcc atgcggttta gcgttctgtc gcgggagcag atcttcagcg agctcaagcg
     1261 cgagctttgt gctttgttcg agctcacgcc cgagcagatc acgccgaagg cgcgcctgta
     1321 cgacgacctc gacctcgaca gcatcgatgc cgtcgatctc gccgtgcgct tgcagaacct
     1381 gacgaagaag cgcatcaagc cggagcagtt caaggctgtt cgcaccgtcg aagacgtcgt
     1441 cgatgccgtc gagcagatgc tgcaggccta gcggccgcgg ccttgcaccg gcggcaccgt
     1501 gaggctcttc cccacggtcg gtacgctggt ctcggttgcc gtggtcctcg ctctcgcggc
     1561 ttatccgttg gtcgtctata ccaccatcgg ccgtttcggg ccgaccggcg tcgccgcgct
     1621 gctcgcggcc gtttgcctgg cccgcctagt gatattgaaa ctgcgcgcgc cggcgtcgtt
     1681 cggcggcggt tacctgacgg ccgtctgtgt gggcggcttg ctgctcgccg ccgcaagctt
     1741 ttggctcgat agcacgggag ccgttcttta ttacccggtg ctcgtcaatg ctgcgctgct
     1801 cgtggtgttc gcggcgagcc tcgtgagccc gccgagcgcc atcgagcgca tcgcacggct
     1861 gcgcgatccc gatctgccgc cggcggccgt cgtttacacg cggcgcgtca cgatcgtctg
     1921 ggccgtgttc ttcgcgctga acggcgcggc cgcactgtac acggcgctgt gcacgacgct
     1981 cgagacctgg gccctctaca acgggttgat cgcgtacgtg ctgattggag ctttgttcgc
     2041 cgccgagctc gcgatccgca cggccgtgaa gtcgaggctc cagcgatgac gcctagccga
     2101 ctcgccgctc tggtcgcgag cgcgaccgat cacctcgtgt gcttcgagcg cgacggcacg
     2161 gagcgcagtt ggcgcgagct cgttgccgct gccgcgagcg tcgccgcgca gctgcgctcc
     2221 gtcgacggcg agcgctgggc cctgaattta ggcggcgggt tcgaggccgc cgccgcgctg
     2281 ctcggctgtt gggcagccgg caggacgccc gtcattgcgc cggcaacgtt gctgacgacg
     2341 ctcgacagtg ccgcgctcga cggtgtcatc gagctcgccg gcgatgcgac gccggcaccg
     2401 gctcgcatcg tctggcaaga cctgacgccg acgcgtgacc cgctgccgac gatctcggcg
     2461 cgagccgccc tcgtgctgta cacgtccggc tccaccggcg tgccaaaagc ggcgcggcgc
     2521 aggctcttca acatcgaaag cgagctcggc gccctcgagg cggtgttcgg tcaaagactc
     2581 gggcgcgtcc ccgtcttcgc aacggcctcg caccgccacg tctatgggtt gttgttcaga
     2641 accttgtggc cgctgctcga gcgacgacca ttcgcgacct tcgattatga gtatcccgag
     2701 caactgctgg gcacggccag ccgcggcaac gtactgatct cgagtccggc gatgctgaag
     2761 cggatcggcc acctgcctgc gggctcgggt caatggcgcg ctatcttcag ctccggcggc
     2821 ttcttaccgc ccgaggcggc gcgcgacgtc gagcgcgtgc tcggcgtcga gcccgtcgag
     2881 gtgctcggca gcacggagac gagcggcgtc gcgtggcgca cgccgtcgac gtcgggcttc
     2941 gagctcttgc cctcggtcga ggtccgcgcg gctgcggacg acctgctcga ggttcgctcg
     3001 ccgttcgcgg gcgcggacgg ctggcaatcg atcggcgacc gcgtcgcgtt tcgtggcgac
     3061 ggccgattcg atcttttggg ccgcgccgac cgaatcgcga agatcgagga caaacgcgtc
     3121 tcgttgtcgg agatcgagcg gcgactgctc gagcacgcgt acgtcaagga cgccgtcgcc
     3181 gtcgcgctcg aggacgacgc gcgacagtac gtcggcgcag tgatcgagct gacacagatc
     3241 ggccgcgagg caatcgcagc gcacggaaag cgcgcagtcg gcagcgcgct gcgtacgtcg
     3301 ctgcgcgggc gcatcgacgc cgtagcgctg ccgcgcgcat tccgctatcc gtcggcgatg
     3361 cctgtcgacg cgcagggcaa gcggcagata gcggcgcttg ctcgcctatt cggccgcacg
     3421 ccgtgaccac ggagcccatc gtcgtcagtc aacgtgtggc gccgcccgac gcggagctgc
     3481 acctcacgat tccggccgat ctcgattatt tccgcggcca cttcgatggc gcgcccgtcg
     3541 tgccgggcgt cgttcaggtc aaatgggccg tggaggccgc tcgccgccac ctcggtgctg
     3601 ccggcgaggt ctcagggatg gagaatctga agttccaaaa ggtgctggtg ccgggcgcgg
     3661 cggcgacatt gacgctgaag tgggttgccg ccgaccgcaa gctgtacttc tcgtatcacc
     3721 gcgatgccga ccggttcagc agcggccggc tgttgtttcg atgagcgccg tgaaggtcgc
     3781 gatcgtcgtt cccgtctaca accatggcga gccgctgcgc gcgacggccg ctcgactcgc
     3841 ggagtttggc ctgcccgtgc tgatcgtcga cgacggcagc gacgaggcga cgaagtgcgc
     3901 gatcgccgag gtcgccgcgc gttatcgata cgaagcagtg acgctgccgc gcaacggcgg
     3961 caaaggccaa gccgtcgtgg cgggtctgcg cgccgcggca gcgcacggct atacgcacgc
     4021 ggttcaggtc gacgccgacg gccagcacga tctcgctgat ctgccgaagt tgctggcggc
     4081 cgccaaagaa aacccggccg cgttgatctg cggcgagccg cgcttcgacg cgtcggtgcc
     4141 gaaagcgcgc ctgtatggcc gcaagctcac ggcgttttgg gtggcgatcg agacggtgtc
     4201 gctgcgcatg cccgacacga tgtgcggcta tcgtgtctat ccgctggccg cgacgctcgc
     4261 gatcatcgat tccgtgacga tcggccggcg catggatttc gatatcgaga tcgtggtgcg
     4321 tctctactgg cacaacgtcc cgatcgtcaa ggtgccgacg gccgtcatct atcccgaagg
     4381 cggcacgtcg aacttccgca cgctcgccga caacgcgctg atctcgaagc tgcacacaaa
     4441 gctgacgttc ggcatgttgt ggcgcttgcc gatgctcgtc acgcggcgcg tcggccatgc
     4501 tttgcactgg gccgatctcg gagagcgcgg cggcgtcgcc ggcatgcacg tgctgttcgg
     4561 cgtctatcga tttttcggcc gtcgtgcgtt cacggcgctg ctgtatccgg tcgtgggcta
     4621 tttttccgtt gcggcgcgca gcccgcggcg cgcgtcgcgc gagtacctcg ccgccatccg
     4681 cgcgcggctc gtcgagctcg gccgcccagt gccgccgcag ctatccgtct ttcaccacgt
     4741 cctcgagttc ggcaacggag tgctcgacaa ggtcgccatg tgggccgacg cgatgcccaa
     4801 tcgcgggatc acgcaggagg acttcgagat gctcgagcgg ttccgcaagg agtcgcgcgg
     4861 cggcgcgctg ttcatcggct cgcacctcgg caatctcgag atgctgcgcg cggtcggcga
     4921 cgtacagaag atgacggtga acgcactcgt gttcacgcgt cactcgccga agttcaaccg
     4981 cgtgctggcg gcggtcagcc cgaagacact agagcgcatg atccaggtcg attcgctggg
     5041 tcccgaggcc gtcatcatgc tccgcgacaa gctcgcgtcg ggcgagcacg tcgcgatcgt
     5101 cggcgatcgc gtttcggtgc ggcacaagga gcgctcggtg cgcgctccat tcatgggccg
     5161 gcccgcgccg ttcccggagg gtccgttcat tctcgcgagc ttgctcgagt gccccgtgta
     5221 tctactgttc tgcttgaaga tagaggggcg ttaccgcgtg ttcatggagc cgttcgcgga
     5281 cccgctcgtg ctaccgcgcg gcggacgaca gcaagcgctc gagcgcgccg tcgagcgtta
     5341 cgcggcgcgg ctcgaagcgc attgcttgct ggcccctacg caatggttta attttttcga
     5401 cttctggggg caagccggcg gccactgagc ggccaggggc tcaccgcttg ggggaacaaa
     5461 tcgcgtgttg cagtcggaaa tcgagctcga ggtgccgttc cacgactgcg acccgatggg
     5521 cgttacgtgg cacggttgct acgtggccta cctcgagctg gcccgctcgg cgctgctgaa
     5581 tcgcattggc tacaacttcc gacagatgac cgcttccggc ttcgcctggc cggtcgtcga
     5641 cctcaaggtc aaatacgtca agacgacgac cttcggtcag cggctcgtcg tcagcgcggc
     5701 gctcgaggag tacgagaatc gcctcaagat cgcctacacg atcgccgatc ggcagtcggg
     5761 cgacatcgtg accaaagcga gcacgacgca ggtggccgtc gatcaggctc gcggcgagat
     5821 gtgcttcgtg tcgccgcaaa cattcatcga caaagtgcgc gaggctctca cggcggcacc
     5881 gcgcgccggc ggtcagcgag ttttgtgaaa ctgtacttga acgacatggg cgtcatcggc
     5941 ccgcttggcg ccggtaaacg cgaggtgctg tcgaacctgc tcgccggcga caccggcggc
     6001 atggtgccgt acagcgagtt gctgacgggg cgagcgacga cgatcggccg cgtccgcagg
     6061 gagcccgagg ccgtgccccg cgagctggcc gagctcgact gccgcaacaa ccgtctgctc
     6121 cagcatgcgc tcgacgagat ccgcgtgtcc gcgtccgcgc tcggtgctgc cgtcgggccg
     6181 gggcgcgtcg cggtggtgct cggtacgagc acctcgggga tcgcgtcagg agaagaggcc
     6241 gcgcaagctt tggcacgtga cggtcgaatg ccgcctcact tccactacaa gcagcaagag
     6301 ctcggaacgg cggccgagtt cgctgcccgt tatctcggtc tcgacggcct gcgttacacg
     6361 atctccactg catgctcctc gtcggcgaag gcgttcgctt ctgccgcgag gttgttcgcc
     6421 gccgatcttt gcgacgctgc tatcgtcggc ggcgtcgata gcttgtgtct gctgacgttg
     6481 aacggtttcg acgcgctcga gtcgctcgcg gccgagcggt gcaatccgtt cagcgccaac
     6541 cgccgcggca tcaacatcgg cgaaggcgcc tgcgtgttcc tcgtctcgcg agacgagagc
     6601 gcgattcgtc tctatggcgt cggcgagtcg tccgatggtt atcacatctc ggcgcccgac
     6661 ccaagcgggc gcggcgccga agccgcaatc cgcgcggcgc tcgcggacgc gggcctggcg
     6721 gccggcgacg tgggttacgt caatctgcac ggcactgcca cgcccaagaa cgaccagatg
     6781 gagagcgagg tcatggcgcg cgtgttcggc ctcgacgtgc cgtgcagctc gacgaaatct
     6841 ctgatcggtc acgccttggg ggccgcgggc gcccaggagc tcggcctctg ttggctgttg
     6901 ctcagcgacg cgaacggcgg ccggcgtttg ccggggcacg tgtgggacgg cgtgcgcgac
     6961 gcgagcttgc cggcgatcaa tctcgtcggc acggacgcgc gtttcgagcg cgacgtgttc
     7021 gtctcgaact cgttcgcgtt cggcggcagc aacgcggcga ttgcgatagg cagagctcga
     7081 tgaacagcgc tgcgattcca atcggcgagc tgctgccgca cggtcccgag atgacgctga
     7141 tcgagcggct cgtcgagtac tctgcgcagc gctccgtcgc gacagtcacg atcacgccgc
     7201 ggagccgttt cgtcgagccg ggcggcgtgc cctcgtgggt cggcatcgag tacatggcgc
     7261 aaacgatcgc cgctcacgcg ggttacgagg cgcggctccg cggcgagccg ccggccatcg
     7321 gcttcctgct cggcacgcgc gcctatcgca gcgacgtcgc agcgttcgcg ctcggcgcga
     7381 agctgacgat caccgtcgag ccgctcgtga ccgacgcgaa gctcgcggcg ttccaatgct
     7441 cgatcgcgac gcagcgcgtc gtggcgacgg ccgtcgtcaa cacctatcaa ccggccgccg
     7501 gcgagctcga cgccatgaga cgcggaacgg tagcgccatg acacgctcgg tgctcgtcac
     7561 cggagcgagc aagggtctcg gcgccgcgat cgcgctcgcg ctcggccgcc agggtttcac
     7621 gatcgccgtg cattaccgca gcgacgcaga gggcgccgcg cgcacggccg agggcattcg
     7681 cggcgcgggc ggcgagtgcc gcgtgctcca gttcgatgtc actgatcgcg ccgcttgccg
     7741 caccgcgctc gaggcggaca tcgcggcgca cggcgcttat tacggcgtcg tcaacaacgc
     7801 cggcgtccac cgcgacaatg cgtttccgct gttgacggag cgggattggg acgaagtcat
     7861 acgcacggat ctggacgggt tctacaatgt gctccagccg tgcgtgatgc cgatgatcca
     7921 agcgcgccgc ggcggccgga tcgtgacgct atcgtccgtg tccggcgtgc tcggcaatcg
     7981 cggtcaagtc aactacagtg ccgcgaaagc cggcgtcatc ggtgcgacga aggcgctcgc
     8041 gctggagctc gcgaagcgca aaatcaccgt caattgcgtc gcgccgggat tgatcgacaa
     8101 cgtcgagctc ggtgacgaag acaaggaacg tatcttggag atgatcccag tccgccggtt
     8161 ggggacgccc gaagaggtcg ccgcgctcgt cgcctacctg atgtccgatc tcgccgcgta
     8221 cgtcacgcgc caggtcatct cgatcgacgg cgggttatcg tgacggcacg cgtcgccgtg
     8281 acgggcatgg cgggtctcac gtcgctcggc caagattacg acgccgtcat cggcgccatg
     8341 gacgcgggcc gcagcggtat ccgcttcatg gaggagtggt cgcgctacga cgggttgaac
     8401 acgcatctcg gcgggccgat cgacgatttc gccttgcccg agcattatcc gcgcaagaag
     8461 atccgctcga tggggcgcgt ggcgagcttg gccgtcgtcg cgacggagcg cgcgctcggc
     8521 gccgccgcgc tggcctcgtc cgacatcgtc acgaacggcg agtgcggggt cgcctacggc
     8581 tcgtcgtcgg gcagccccga tgcaatcgct gatttcggag cgatgctgaa cgagcacagc
     8641 acgcggatga tcaactcgac gagctacatc cgcatgatga gccatacggc ggccgtcaac
     8701 acgagcttgt tcttcaacat tcgcggccgc ttggtgtcga cgagcacggc ctgcacgtcc
     8761 ggcagccagg cgattggtta cgcctacgaa gccgtcaagc acggcgcgca gcgcgtcatg
     8821 gtcgcgggcg gcgccgagga gctgtcggcg acgcaggcgg ccgtgttcga cacgttgttc
     8881 gcgacgagct gcaagaacga tgcaccgacg acgacgccgc ggccttggga ccgcgaccgc
     8941 gacgggctcg tgatcggcga gggcgccgcg acgttgatcc tcgaggattg ggactccgcc
     9001 gtcgcgcgcg gcgcgcgcat cttcgccgag atcgtcgggt tcggcacgaa ctgcgacgcg
     9061 cgtcatgtga ctcagccgac gtccgagacg atggaggagg cgatgcgccg cgcgctgcgc
     9121 gactcggggc tcgacgcggc ggcgatcggc tacgtcagcg gtcacggcac ggcgacggag
     9181 ctgggcgaca tcgcagagag ccatgcgacg ctcgcggtgt tcggcgaggg aaccccgatc
     9241 agctgcatga agagttactt cggtcacacg ctcggcgcgt gcggagccca ggaagcgtgg
     9301 ctcgcgatcg agatgatgaa ccgcgaccat ttcgcgccga cgctgaatct cgaccacgtc
     9361 gacccgcgct gcgcgagcct cgactacatc gtcggtgcgc cgcgcacgct ctccaccgaa
     9421 tacgtcatga gcaacaactt cgcgttcggc ggcatcaata cctcgctgat cttccgccga
     9481 gtctcgtgac gggcgacgtc cgcgacgctt cgaagtccac gggcgtcgtc cacgtctatt
     9541 acgccgaccg gcgcgcgttg cctgccgccg ccgtcgaagc gctcgtgaca gccgaagaca
     9601 ggcagcgcgt gacgggcacc atgcatccgc gtcgcaccgc cgagtacctc gcgagccgcg
     9661 cgctgctgcg gcactcgttg gcgcggcaca cggcgcgcag cgccgcagaa ttgcgcatcg
     9721 aaacgtcggc cgacggcaaa ccttcgtgcg tcggcggccc cgaggtcagc gtgtcccaca
     9781 gcggtgacat cgtcatgtgc gcgctcgcgg ctgcgggcgc ggttggcgtc gacgtcgaga
     9841 ccgcggggcc gcggtccgtc gcggcagtcg ccgagcgctt cttcacggcc gcggaagcgc
     9901 gctgggtcgc ggcgcagccc gagccgcggt tccgccagct ctgggtcctc aaagaagcgt
     9961 acttgaaggc gctcggcgtc ggtctcgcgg gcggcttgag ctcgctcgag tgccgcgtcg
    10021 agctgccgtc gatcgtcgca cgagtggcgg gcgcccccct tcgcggactt ccagggggcg
    10081 agggcggtgc accgcagctc gcgttgctgc aaggcaaggg ctgctccgtc ggagtcgcgt
    10141 cgttggccgc gactccgctc gagctggttc tcgagcatgt ggccctcgac ggcagcgccg
    10201 acgccgtcgg accgctcgcg ctgctcgcta ggacgtgagc gtgcgccgat gaacggcgag
    10261 cgtcatcgaa ggcgttggcg gctcgctacc agttggcgtc ctcgttcggc ttcgctggat
    10321 ccgccgtgat gttcagcacc gcgcagtggt gatacgagaa gccgccctcg cgcacgccgg
    10381 ggtgctcgag catgacctga atcacttgca gctgacagcc ttcgcacgtg atgttcggga
    10441 tcgtgatctc ggtctcccag ttcttctcgg gcttctcggt gtgcggccag agattgtcga
    10501 ccagaatcgg cggcatcggc gtggtcatga tgaacgccga ctgcgaacgc aggccgcgtt
    10561 ccgtctcgtg catcgtgacg gccgggtcga gcggcagcag gttgcgcttg cgcgctagcg
    10621 agatgcggaa gtggcccggg tgaaagatcg tttcgttgac gacgaccttg agcttgctgc
    10681 cgccggtgac ggccgtcatt gcagtggtcg gctcgccggg atcggcgaga gtgccgccgc
    10741 acggcgccag cttctgcgga tcgccgcgct cgtcctgctc gagccacgcc gcgggcgact
    10801 cgagcttgaa atgcgcgtag acggccggag ctacgcccaa tgttgcgatg ccggcgacgg
    10861 gaaacgcgag cagccacttc ttcaagacac acccccgctt cgagttgttg atcgcgcgca
    10921 ttctagccgc gccgcggggc ggttgcgctg aagaattgtc gcaagtggcg tgttccttgc
    10981 agccggcaac cggtatgctg gcgcccgcgc cgagcgcgcc gagcacgcaa cggagtgcaa
    11041 cgatgacagc gaagaccatc aaacgcgcgc atcccgcgat gcgggacgac atcgccgatc
    11101 tgacgacgcg gcgccccgtg ccggggcagg acgtcgagca gatcgacccg ttcttgttct
    11161 tgaatcatca cggcccgcag gtctacgcgc cgcgcaatcg cggcctgccg ttcggcccgc
    11221 atccgcatcg cggtttcgag accgtcacgt ttatcctgac cggcgagctc gcgcatcgcg
    11281 acagcgccgg ccacgagagc atcatcaagg aaggcggcat tcagtggatg acggccggca
    11341 gcggtctcgt gcacgccgag atctcgcccg aggagttcat gcgcacgggc ggcccgctcg
    11401 agatcctgca gctctgggtc aatctgccgc cgcggctcaa gatgacgcag ccgcgctata
    11461 tcggcttgca gcgcgacgag atcccggcgg tcccgacgcc ggacggcaaa ggcgtcgtga
    11521 atctcgtctc gggcgagtac ctcggccgca agggcgccat gcagccgctg ctcgacatct
    11581 tcatgtcgac ggtcgagctc gaagccggcg cgcgcgtcga gttctcgggc gtcgcgcaac
    11641 gcaacgtatt tctctacgtc gtgcgcggct cgatcaaggt cgcgggcgag ccggcgccgc
    11701 actggcactt gctcgagctc agcgatgaag gcgacaccgt cgcggtcgag gccgataccg
    11761 aagccgtgct gcttttcggc catgccgagc cgatcggcgc gcccgtcgtc gcgtacggcc
    11821 cgttcgtcat gaacacgcgc gacgagatca tgcaggcgat tcaggattac caggccggca
    11881 aattcaacgc tattccggcc ggacgcgcca cggcgtgaag ccgcgcaaac cgctgcgcaa
    11941 cgtcattttc gacttcggcg gcgtcctcgt gacgtggcgg ccgcaggaga tcatcgactc
    12001 gttctacgag gagccggcgc tgcgtgaagc gctgcgcacg cacgcgttcc agcacgacga
    12061 ttggctcgac atggatcgcg gcacgctcga cgaagcggcc gtggcgctgc gctgcgccgc
    12121 gcgcatggca aggcctgaag ccgagctcaa ggcgctgttc gatcacgttc gcgcggcgct
    12181 gcagccgatc gatgcgacgg tcgctttttt gcgcgagctg cgcgagcggg ccgacctgaa
    12241 gctctatggc ttgtcgaaca tgtcggcgac gattttcgcg taccttgcgg gccggcacga
    12301 cttcttcgag ctgttcgacg gcatcgtggt ctctgcccac atcaaactgc tgaagcccga
    12361 gcccgagatc tacgtgcacc tgcgcgaccg gttcgacctc gactttgccg aatccgtgtt
    12421 catcgacgac atggcgcgca acgtcgaaag cgcgcgcgcc gtgggcttgc cctcgatcca
    12481 gttcttgagc acggaccagg tccgccgcga gctagccgcg ttgctttgag cgcactacgc
    12541 tgttgccgtg gaagtccccg ggcggcgatg gcagagtacc gctcagatgc gcgcgaagtc
    12601 tctgcttctc ggaattgcgg tctgcctgct gggcgggctc gcgagcgcgc agttcggccg
    12661 ccgggcgccc gagccgggcg gcgcgccgcc cgaagccgaa tttcacatgg cgcggctcgc
    12721 gtactacgcc cgcggctgcg ccggctcgcg cggctactgc aatccgtggt gggccatcga
    12781 ctatccgtac gccgaggcgc acttcctgcc ggccgtcgag cgcatgaccc gaatcgaagt
    12841 ggctcccgac agccgccata tcacgatcga cgacgaatac ctgttcgact acccgtggtt
    12901 attcctgcag cagcccggcc agggcaactg gcatccgcaa ggcgaggagc tcgagcggct
    12961 gcgcgagtat ctcgtgcgcg gcggcttcct cgtcgtcgac gattttcaca acgagtacga
    13021 atggcaggcg gtacgcgatg ccatggccga cctgttgccg gggcgcccga tcgtcgacat
    13081 tcccgacgac gacgcgctgc accacatcct gttcgatctc gacgggcgca cgcagatccc
    13141 cggcgagcgg catctgtacc gcggcatgga agggccgccg cactggcgcg gcatctatga
    13201 cgatcgcggc cggctcatgg tcgcgctgaa ccacaacagc gacatgggcg acgcgtggga
    13261 gcacgccgac gacgcgcact acccgacgcc gatgacggcg acggcctatc ggttcggagt
    13321 caactacgtc atctacgcga tgacgcattg agatgccggc gaagctctat ctcgacgacc
    13381 ttgccgtcgg ccgcaagttc gtgagcagcg agaccgcggt cacgctcgag gcgtgcaagg
    13441 cattcgccgt gcaattcgat ccgcagccgt tccacctc
//
LOCUS       DATLEE010000602         5195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4424961, whole genome shotgun sequence.
ACCESSION   DATLEE010000602 DATLEE010000000
VERSION     DATLEE010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4424961"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VM692_13085"
     CDS             <1..197
                     /locus_tag="VM692_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53152.1"
                     /translation="AAPPVAEGAEPAATPPAATPPAADPSAEAARINGKVGGWRYKIA
                     GFQYDQLTRHMADLLKAPAA"
     gene            285..1832
                     /gene="ilvA"
                     /locus_tag="VM692_13090"
     CDS             285..1832
                     /gene="ilvA"
                     /locus_tag="VM692_13090"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF006674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HUQ53153.1"
                     /translation="MKTPQKSYPIAASYAPLIARARVYDVAIESPLDRAPRLSARLGH
                     DVLLKREDLQAVFSFKLRGSYNKISQTPDEKLANGVICSSAGNHAQGVALAARRRGLR
                     AVIVMPTTTPSIKVDAVVALGGEAVLHGATYDDASAHARELEAEQGLCFVHPFNDPDV
                     IAGQGTVGAELERQWPKVPDVVFAPVGGGGLISGIGSYLKHRYPGIKIVGVEPVESAS
                     MYESLRAGRIVTLERVGTFADGVAVKRVGDETFRVCSEVVDEIILVNTDEICAAIKDI
                     FEDVRAVAEPAGALAVAGMKRYAQRTSARGQTFIAVNSGANMNFGRLRHVTERAEIGE
                     QREALLAVEIPEQPGAFLHFCETIGDRNITEFNYRYAPGARARIFVGVSLQRGLAEAR
                     EIVALLERQAYPVLDLSGNELAKLHIRHLVGGQVPGLKNELLYRFEFPERPGALHAFL
                     QAIGDLWNISLFHYRNHGSDYGRVLAGVQVPEADRAEFARHLAKLGYTHVNETENPAY
                     RLFLQAQ"
     gene            complement(1905..2537)
                     /locus_tag="VM692_13095"
     CDS             complement(1905..2537)
                     /locus_tag="VM692_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53154.1"
                     /translation="MLLPKLEAMLHELYALEVGYAVSDFLITDALLAGSLDAGGRQSD
                     EKLLIAESNGEADVALYLEQELLDRLARRDPLLRLDSENLADFWSALEGVSHFIYYAW
                     NAARDKPVSLFELELQAEVDKFVTTAILLRAQTGREPSGLHGWLFDSPTLAAELDDEE
                     RERYRRANRYAARYCSRLSPDRSDAEAVQSELRRFYRLSQASKLAHIDSE"
     gene            complement(2541..3164)
                     /locus_tag="VM692_13100"
     CDS             complement(2541..3164)
                     /locus_tag="VM692_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53155.1"
                     /translation="MRAPADRVHAVASLQEFFKDSVAAAMAKQGVAADDHTAYYVVNL
                     LTLFGRHETLYDQGKPGPGLQPLAVLLAAAADSADRETRNALLRRVGDTSLFVAGFLG
                     DGFARKLIDVDYYIDMGGAAYGRLHDNVRGTREGRAFGSVFAELATKFGEFVDVLAEI
                     RDSGKAAAIDVLRLYELWLRTGSRRAGRLLREHGLEPNATLNVATRH"
     gene            complement(3267..3701)
                     /locus_tag="VM692_13105"
     CDS             complement(3267..3701)
                     /locus_tag="VM692_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53156.1"
                     /translation="MLKQRHEAQLKCALTEAFSALVDVAGKGRWGGASLALGAALPQT
                     GCRYEQQRGKVLRRGKVLACLRPVSITLQETLFDPPCCVQLRLHWRLEPTEGGACVLL
                     DARYSLNGAAALRRRHWYERIHGHCARMLCALESRVVQPEET"
     gene            complement(3798..4769)
                     /locus_tag="VM692_13110"
     CDS             complement(3798..4769)
                     /locus_tag="VM692_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HUQ53157.1"
                     /translation="MTRAILFDSFGGPEVLQYREVELAAPGEGQARIAHRAIGVNFID
                     VYHRTGLYPLKLPSGLGGEGAGVVTAVGPGVTTVVPGDRVAYTAPAPLGSYADERLVD
                     AKWLVKLPPNVGEQTGAAMMLKGLTAWYLLKRSYRVAAGDWVLLYAAAGGVGLIAAQW
                     ARELGVRVIGVVGTEAKRELALRAGCEHVLLDGDDIPRRVREISGGGVPVVYDSVGRT
                     TFYQSLDCLRPHGLLVSFGNSSGKVEPFALQELMKRGSLYVTRPTLQDFIRDPRDLES
                     GAAELLGLVERGRVKIAVNQTYALRDAAQAHRDLEARRTTGCTVLIP"
     gene            4834..>5195
                     /locus_tag="VM692_13115"
     CDS             4834..>5195
                     /locus_tag="VM692_13115"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUQ53158.1"
                     /translation="MSEHLVIVGGGQAAAQAIQSLRQQSFAGPITLLSDEPYPPYQRP
                     PLSKKYLAGELPRERLFLRPAAFYTEKGVTLEQNARVEELDAVGRRMRLRDGRTLSYD
                     RLLLATGSRVRTLDVPGAS"
BASE COUNT          892 a         1799 c         1700 g          804 t
ORIGIN      
        1 cggccgcgcc gcctgtcgcg gagggcgcag agccggcggc gacgccaccg gccgcgacgc
       61 ctccagccgc ggacccgagc gcggaagcgg cgcgaatcaa tggcaaggtc ggcggctggc
      121 gctacaagat cgccggcttc cagtacgacc agctgacgcg ccacatggcg gacctgctca
      181 aagcacccgc ggcctagccg ctccacgacc tcgcccgacc gctgtgcgcg cgctgcgcgc
      241 ggggctcgcg cccggtacac tgcgtgccgt ttgcgccaac ccctatgaaa acgccgcaga
      301 agtcataccc gatcgccgcg agctacgcgc cgctgatcgc gcgcgcgcgc gtctatgacg
      361 tagcgatcga gtcgccgctc gaccgcgcgc cgcggctgtc ggcgcggctt ggccacgacg
      421 tgctgctcaa gcgtgaagac ctgcaagccg tgttctcgtt caagttgcgc ggctcgtaca
      481 acaagatctc gcaaacaccc gacgagaagc tcgcgaacgg cgtgatctgc agctcggccg
      541 gcaatcacgc gcaaggcgtc gcactggccg cccgcaggcg cgggctgcgt gccgtcatcg
      601 tcatgccgac cacgacgccg tcgatcaaag tcgacgccgt ggtcgcattg ggcggcgaag
      661 ccgtgctgca cggcgccact tacgatgacg cgagcgcgca tgcgcgcgag ctcgaagccg
      721 agcaaggtct ttgcttcgtg catccgttca acgatcccga cgtgatcgcc ggtcaaggca
      781 cggtcggcgc cgagctcgag cgtcagtggc cgaaagtgcc cgacgtcgtg tttgcgcccg
      841 tcggcggcgg cggtctcatc agcggcatcg gctcgtatct caagcatcgc tatcccggca
      901 tcaagatcgt cggcgtcgag cccgtcgagt cggcgagcat gtacgagtcg ttgcgtgcgg
      961 ggcgcatcgt cacgctcgag cgcgtcggca cgtttgccga cggcgtcgcc gtgaagcgcg
     1021 tcggtgacga gacgtttcgc gtttgtagcg aggtcgtcga cgagatcatc ctcgtcaaca
     1081 cggacgagat ctgcgcggcg atcaaggaca tcttcgagga cgtccgcgcc gtcgcggaac
     1141 cggccggcgc gctggccgtg gccggcatga agcgctacgc gcagcgaaca agcgcgcgcg
     1201 ggcaaacgtt catcgccgtc aatagcggcg ccaacatgaa cttcggccgg ctacgccacg
     1261 tgacggagcg cgccgagatc ggcgagcagc gcgaggcgct gctggccgtc gagatccccg
     1321 agcagcccgg cgcgttcttg cacttctgcg agacgatcgg cgaccgcaac atcacggagt
     1381 tcaactatcg ctacgctccg ggcgccagag ccagaatctt cgtcggcgtg tcgctgcagc
     1441 gcggcctcgc cgaggcccgc gagatcgtgg cgttgctcga gcggcaggcg tatcccgtgc
     1501 tcgatctgag cggcaacgag ctcgcgaagc tgcacattcg ccatctcgtc ggcggtcaag
     1561 tgccgggcct caagaacgag ctgctgtatc gcttcgagtt ccccgagcgc cctggcgcgc
     1621 tgcacgcgtt cctgcaggcg atcggcgact tgtggaacat cagcctgttt cattaccgca
     1681 atcacggctc ggactacggc cgcgtgctgg ccggagtgca agttcccgag gcggaccgcg
     1741 cggaattcgc gcgccacctc gcgaagctcg gctacacgca cgtcaacgag accgagaacc
     1801 cggcttaccg gctgttcttg caggcacagt aggcgtgccc tgacccaaca cgcgcgagcg
     1861 accttacggc gtcgcccagt gccgcggccg ccggcgagcg tcaactactc ggaatcgatg
     1921 tgggcgagct tggaggcctg cgacaaccga tagaagcggc gcagctcgct ctggacagcc
     1981 tcggcatccg acctatccgg cgacaaacgc gagcagtacc tcgcggcgta gcggtttgcg
     2041 cgtcgataac gctcgcgttc ctcgtcgtcg agctcggcgg ccagcgtcgg cgagtcgaac
     2101 agccagccgt gcaacccgct cggctcgcgc cccgtttgcg cgcgcagcag gatggccgtg
     2161 gtcacgaact tgtcgacctc ggcttgcagc tcgagctcga acagcgacac gggcttgtca
     2221 cgcgccgcgt tccacgcgta gtagataaag tgactgacgc cttcgagcgc agaccaaaag
     2281 tcggccagat tctcactgtc gagccgcagc agcggatcgc ggcgcgcgag ccgatcgagc
     2341 agctcctgct cgagatacaa cgcgacgtcg gcctcgccgt tcgactcggc gatcaacagc
     2401 ttctcgtcgc tctggcggcc acccgcatcg agcgagccag cgagcagcgc gtcggtgatc
     2461 aggaaatcgc tgacggcgta gccgacctcg agcgcgtaca gctcgtgcag catcgcctcg
     2521 agcttcggca gcagcacgcg tcagtgccgc gtcgcgacgt tcaacgtcgc gttcggctcg
     2581 agcccgtgct cgcgcaacag acgcccggca cgccgactac ccgtgcgtaa ccagagctcg
     2641 tagagacgca gcacgtcgat ggctgcggct ttacccgagt cgcggatttc ggccagcacg
     2701 tcgacaaact cgccgaattt ggtcgcgagc tccgcgaaca ccgagccgaa cgcgcgacct
     2761 tcgcgcgtgc cgcgcacgtt gtcgtgcagc cggccataag ccgcgccgcc catgtcgatg
     2821 tagtaatcga cgtcgatgag cttgcgtgcg aagccgtcgc cgaggaagcc tgcgacgaac
     2881 agcgaggtgt cgccgacgcg ccgcagcaac gcgttgcgag tctcacgatc ggcgctgtcg
     2941 gccgcggccg cgagcaggac cgccagcggt tgcaggccgg gccccggctt gccttggtcg
     3001 tataacgtct cgtgacgccc aaacagcgtc agcagattga cgacgtagta ggccgtgtgg
     3061 tcgtcggccg cgacgccttg tttcgccatg gcagcggcga cggagtcctt gaagaactcc
     3121 tgcagactcg caactgcatg cactcgatcg gccggtgcgc gcaacaagct ctccctgcaa
     3181 ctcaggctca gcactgccgc acatacggca aagcgtgccg ttgctatcga gtatagagtg
     3241 ccgccgcgtc gaatctgcaa acggcttcac gtttcttcgg gttgcaccac gcgggattcc
     3301 agcgcgcaca acattcgcgc gcaatgaccg tgaatccgtt cataccaatg gcggcggcgc
     3361 agcgcggccg cgccattcag cgagtagcgt gcatcgagca gcacgcaggc gccgccctcg
     3421 gttggctcga ggcgccagtg caagcgcaac tgcacgcagc aaggcggatc gaacagcgtc
     3481 tcttgcagtg tgatcgacac agggcgcagg caggcgagca ccttgccgcg gcgcagcacc
     3541 ttgccgcgct gctgctcgta gcggcaaccg gtctgcggca gggccgcgcc cagcgcgagc
     3601 gaagcgccgc cccagcggcc tttgcccgcg acatcgacca gcgcggagaa agcttccgtc
     3661 aacgcacact tgagctgcgc ctcgtggcgt tgcttcagca tggcggtcct ccttgaccag
     3721 cggaagagca acgctagctc cgcgcgcggc gtcgggcgac gcgcaaattc cggcgaagtg
     3781 cggagaaact tcgcgcgcta cggaatcagc acggtgcagc ccgtcgtgcg acgtgcctcg
     3841 agatcgcgat gcgcttgggc ggcatcgcgc aacgcgtagg tctggttcac ggcgatcttg
     3901 acccggccgc gctcgacgag ccccaatagc tccgccgccc ctgattcgag gtcgcgcgga
     3961 tcgcgaatga aatcttggag cgtcggcctc gtcacgtaca acgagccgcg tttcatcagc
     4021 tcttgcagcg cgaagggctc gaccttgccg gacgagttgc cgaagctcac gagcaggccg
     4081 tgcggccgca aacaatcgag cgactggtag aacgtcgtgc ggccgacgga gtcgtaaacg
     4141 acgggaactc cgccgcccga gatctcgcgc acgcgccgcg ggatgtcgtc accgtcgagc
     4201 agcacgtgct cgcagcccgc tcgaagcgcg agctcgcgct tcgcctcggt tccgacgacg
     4261 ccgatgacgc gcacgccgag ctcgcgcgcc cattgtgcgg caatgagccc gacgccgccg
     4321 gccgccgcgt acagcagcac ccagtcgccg gccgcaacgc ggtaactgcg cttcaagagg
     4381 taccaggccg tcaggccctt cagcatcatc gccgcgccgg tttgttctcc gacgttcggc
     4441 ggcagcttca cgagccactt cgcgtcgacg agccgctcgt cggcatacga gccgagcggc
     4501 gccggcgccg tgtacgcaac acgatccccg ggcaccacgg tcgtgacacc gggcccgacg
     4561 gccgtgacga cgccggctcc ctctcctccg agcccgctcg gcagcttcag cggatagagg
     4621 ccggttcgat gatagacgtc gatgaaattc acgccgatcg ctcgatgcgc gatgcgggct
     4681 tgaccttcgc cgggggcggc gagctcgact tcgcggtatt gcaagacctc gggaccaccg
     4741 aacgagtcga agaggatggc gcgtgtcatt ggatctgctc ggccctcggc cgctgctaga
     4801 atcgcggccc cctagcccaa aatatagcac tcgatgagcg agcatctcgt gatcgtcggc
     4861 ggcggccaag ccgcggccca ggccatccaa tctctgcggc agcagagctt cgctgggccg
     4921 atcacgctgc tgtccgacga gccgtatccg ccgtatcagc ggccgccgct gtcgaagaag
     4981 tatctcgccg gcgagctgcc gcgcgagcgc ctgttcctgc ggccggccgc tttctacacg
     5041 gagaaaggcg tgacgctcga gcagaacgct cgcgtcgagg agctcgatgc cgtcggccgt
     5101 cgcatgcgcc tgcgcgatgg gcgcacgctg agctacgacc ggctgttgct cgcgaccggc
     5161 agccgcgtgc gcacgctcga cgtgccgggc gcgtc
//
LOCUS       DATLEE010000603         2384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10051192, whole genome shotgun sequence.
ACCESSION   DATLEE010000603 DATLEE010000000
VERSION     DATLEE010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10051192"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="VM692_13120"
     CDS             complement(<1..2020)
                     /locus_tag="VM692_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HUQ53159.1"
                     /translation="MGKLKTLWRNAVSQRVWEELEVWIGVLLALGAAAVLYATLTAQA
                     AYADEFPTDVGAPTLLFQTADGGYLAAAPLQTDLRLSVAGVVARVRVSQRFRNSGANY
                     IEAVYALPLPDDAAVDRLSMQIGDRIVEGEIQERQRAERIYGEARAAGQRASLVRQTS
                     ANLFTTAVANIGPGETIDITIEYLQTARYDAGEFSLRVPLTLTPRYGASDTPEGSGAL
                     DSAVTAEAAGLESPTAAGLRSAPWHEAYVHAVLAPGMPLEWIGSPSHELTAVHERGPA
                     WSAARESSKVENGAAVAGVLVQPPPAELYVLDTVAPRVPMDRDLVVAWRPQTGVAPGV
                     AALTETVGDTTYALLMILPPAAAHTARAQAREQIYVVDTSGSMAGQSMVQAKAALKDA
                     LGRLNSGDRFNVIQFNSTTSTLYLQPVQFSAGTYKQALAYVAGLTANGGTEIAAAIDT
                     ALSQPTVPGSLRQVIFMTDGAVNSETSLYAAIEQRLGDARLFTIGIGSAPNSQFMRKA
                     AQFGRGTFTHIGNVNDVAEKMTALFDKIEHVALSNVVVDWPEAVELYPHEIPDLYAGE
                     PLVVAASFPARPDKPLLTTAYGQAGGLPWRQPVAGMPSELPGIATLWARRKIETLLDS
                     RVAGTDEALIRKLVVATALEHHLVSPYTSLVAVDKTPARAAVAALERRT"
     gene            complement(2150..>2384)
                     /locus_tag="VM692_13125"
     CDS             complement(2150..>2384)
                     /locus_tag="VM692_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HUQ53160.1"
                     /translation="ILSTSVDAFLREGDFADALTEIQRKYPAVEIGSYPFNRDGRLGA
                     TLVARGTDRALLTRAVAEIVAAIAALGGETHVP"
BASE COUNT          340 a          817 c          838 g          389 t
ORIGIN      
        1 tggtgcggcg ctcgagcgcc gcgaccgcgg cgcgcgccgg cgtcttgtcg acggcgacga
       61 ggctcgtgta cgggctcacg aggtgatgct cgagcgccgt tgcgacgacg agcttgcgga
      121 tcaacgcttc gtcggtgccg gccacgcggc tgtcgagcaa cgtctcgatc ttgcgccgcg
      181 cccatagcgt cgcgatgccg ggcagctcgc ttggcattcc ggcaacgggt tggcgccagg
      241 gcaaaccgcc ggcctgaccg taggccgtcg tcagcagcgg cttgtcgggc cgcgccggaa
      301 agctcgccgc gacgaccagc ggctcgccgg cgtaaagatc gggtatctcg tgcggataga
      361 gctcgacggc ctccggccag tcgacgacga cgttgctgag cgcgacgtgc tcgatcttgt
      421 cgaacagcgc cgtcatcttc tcggcgacgt cgttcacgtt gccgatgtgc gtgaacgtgc
      481 cgcggccgaa ttgggcagcc ttgcgcatga actgcgagtt cggcgccgag ccgatcccga
      541 tcgtgaacaa gcgtgcgtcg ccgaggcgct gctcgatcgc ggcgtacaac gaagtttccg
      601 agttgacggc gccgtcggtc atgaagatga cctgccgcag cgaccccggc accgtcggtt
      661 gggagagcgc cgtgtcgatc gcggcagcga tctccgtgcc gccgttggcg gtgaggccgg
      721 cgacgtacgc aagcgcctgc ttgtacgtcc ccgccgagaa ttgcacgggc tggaggtaga
      781 gcgtcgacgt cgtcgagttg aactgaatca cattgaagcg atcgccgctg ttcaagcggc
      841 cgagcgcgtc tttgagcgcg gccttcgctt ggaccatcga ctgcccggcc atcgagcccg
      901 acgtgtcgac cacgtaaatc tgctcgcgcg cttgtgcgcg cgccgtatgc gcggccgccg
      961 gcggcaggat catcagcagc gcgtaggtcg tgtcgccgac ggtctccgtc agcgcggcga
     1021 cgcccggcgc gacgcccgtt tgcggccgcc aggcgacgac gaggtcgcgg tccatcggca
     1081 cgcgcggcgc cacggtgtcg agcacgtaga gctcggctgg gggcggctgc acgagcacgc
     1141 cggccactgc cgcaccgttc tcgaccttgc tgctctcgcg cgcggcgctc caggcaggcc
     1201 cgcgctcgtg gacggccgtg agctcgtggc tggggctgcc gatccactcg agtggcatgc
     1261 cgggcgcgag caccgcgtgt acgtacgctt cgtgccacgg ggcgctacgc aacccggccg
     1321 ccgtcggcga ttcgagcccc gccgcttcgg ccgtcacggc cgagtcgagc gcgccggagc
     1381 cttccggcgt atccgacgcg ccgtagcgcg gcgtcagcgt cagcggcacg cgcaggctga
     1441 attcgccggc gtcgtagcgc gccgtctgta ggtactcgat cgtaatgtcg atggtctcgc
     1501 cgggcccgat gttcgccacg gcggtcgtga acagattggc gctcgtttgc cgcacgaggc
     1561 tcgcgcgttg tccggctgcg cgcgcttcgc cgtagatgcg ctcggctcgc tgacgctcct
     1621 gaatctcgcc ttcgacgatt cgatcgccga tttgcatcga caaccggtcg acggccgcgt
     1681 cgtcgggcag cggcagcgcg tacacggcct cgatgtagtt cgcgccgctg ttgcggaagc
     1741 gttgcgagac gcgcacgcgg gcgacgacgc cggcgaccga gagccgcaag tccgtctgca
     1801 gcggcgccgc ggccaagtag ccgccgtcgg ccgtttgaaa caacagcgtc ggcgcgccga
     1861 cgtcggttgg aaactcgtcg gcgtacgcgg cctgcgccgt cagcgtcgcg tacaacaccg
     1921 ccgcggcgcc gagcgccagc agcacgccga tccagacctc gagctcttcc cagacacgct
     1981 gcgagaccgc gttacgccac agggttttca gcttgcccat cgaacgactc ccagccgctt
     2041 ctctgggaat cattgagcgc cgcgttaggg cgttttcggc ggcgaatcgt ggccgcgcgg
     2101 cggcgaattg tgatcaattc tggccggcga cgcgccgcag gccgacgcgc tagggaacgt
     2161 gcgtctcgcc gccgagcgcc gcgatcgccg cgacgatctc ggcgaccgcg cgcgttagca
     2221 gcgcgcgatc cgtgccgcgc gcgacgagcg tcgcgccgag gcggccgtcg cggttgaacg
     2281 gatagctgcc gatctcgacg gccggatatt tgcgctggat ctccgtcagc gcgtcggcga
     2341 aatcgccttc gcgcaagaac gcgtcgacgc tcgtggacag tatc
//
LOCUS       DATLEE010000604        11405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3358356, whole genome shotgun sequence.
ACCESSION   DATLEE010000604 DATLEE010000000
VERSION     DATLEE010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11405
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3358356"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /gene="msrA"
                     /locus_tag="VM692_13130"
     CDS             <1..470
                     /gene="msrA"
                     /locus_tag="VM692_13130"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HUQ53161.1"
                     /translation="QDLIRKMAGVVSTRVGYSGGDVANATYRRHGTHAEAIEVVFDPQ
                     RLTYRKLLEFFFQIHDPTTKNRQGNDMGTSYRSGIYYLTDEQREIAEDTIADVDASKL
                     WPGLVVTEVKPAGPFWEAEPEHQDYLLRYPGGYTCHFPRPNWVLPRRAAQRTA"
     gene            475..957
                     /gene="msrB"
                     /locus_tag="VM692_13135"
     CDS             475..957
                     /gene="msrB"
                     /locus_tag="VM692_13135"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HUQ53162.1"
                     /translation="MSTLEAPEPSSLRWKSVVEIARAGNEPPPRRVEKSDAEWRQQLS
                     AEQYHVARGKGTERPFSSEMCALFEPGRYHCICCDTPLFDSTTKFDSGTGWPSFTQPL
                     TRGVVAYHLDTSYGMQRVEALCNVCDAHLGHVFPDGPPPSGLRFCINAVSLRRAKESK
                     "
     gene            complement(1034..2779)
                     /locus_tag="VM692_13140"
     CDS             complement(1034..2779)
                     /locus_tag="VM692_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HUQ53163.1"
                     /translation="MKLRSLTIPLAAAVLLAAGCGRSDGGGSDGVPTPAAAPAASATW
                     DAFRDRFIEGFFELNPSYAVYQGRHEFDGRISDWSTASLQRQMDFLRAAIADAEAFDT
                     TQLSAAQSFERDYLASVARGELFWLEDADQPHANVSWYFDNGLDPGVYTTRPYADAAT
                     RLRAFVAHARAVPVALAQIRANLRTPLPLSFVEFGVAGFNGYAEYYRGDGTAAFASVG
                     DTALQAELAAAAETAATAMTAAAAWLETQRAMATQDFALGAERFARMLAATEDVTVPL
                     DALEALGRADLRRNQDALREACATFAPGATLGDCMAKMAATKSPLGPVAEARTQLPAL
                     KQFLLDTDLVSIPGSEEALVEEAPPYNRQNAAYIDIPGPYEKGLPSVYYIAPPDPSWT
                     AEEQLEYVMGSKDLLFTSVHEVWPGHFLNFLHSNRASLPIARVFYSYAAAEGWGHYAE
                     ELVWEAGFEAGNAESHIGQLSNALLRNCRYVSAIGLHTRGMTQQQSYELFRNECFQDE
                     GNSRQQAARGTYDPAYLNYTLGKLMIRKLRDDWTASRGGRQAWKSFHDTFLSYGGPPI
                     PLVRRAMLGDDSPPL"
     gene            2825..4201
                     /locus_tag="VM692_13145"
     CDS             2825..4201
                     /locus_tag="VM692_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HUQ53164.1"
                     /translation="MAVSPPAAPTLSRTLRSRHVALISIGGIIGAGLFVGSSANIASV
                     GPAVLVSYCVAGGLMFLVMRMLAEMAAANPAALSFADHARAGLGAWAGFACGWLYWYF
                     WVVVVGIEAIAGAAILQTWIPAQVWQIGLVLMVLLTATNLLSTRSYGEFEFWFASIKV
                     AAIVAFILIGGSYALGFSSPTGATFGNLVEHGGFAPFGAAAVLAGVTGIVFSLTGAEI
                     VTVVAAESSEPSQAIAALTTSLIVRILLFYVGSMLVILAVVPWTEIAPGSSPFALALE
                     RMEIPFAATTMNLIVLVAVLSCLNSGLYVTSRMLFGLAAQGDAPRSLVALDARGVPTR
                     AILIGTVFGYLAVLASVASPQRLFTFLVNASGAIMLVIYLLIALAQIRRRRVMERDAP
                     ARLVVRMWLYPWLSYVTVAGIGLVLASMLFRTAERAQLAASALSVAVVLAIFWWKRRR
                     ASSNSEQE"
     gene            4208..6250
                     /locus_tag="VM692_13150"
     CDS             4208..6250
                     /locus_tag="VM692_13150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HUQ53165.1"
                     /translation="MRTSLSALVLVFAATAASAAPPEAPARQLTGLDLFNLQVATDPQ
                     IRPDGAVVAYTRVSYDVMTDSARPSIWLIDVATGQQTPLVTGSGSHSTPRWAPAGKRL
                     AYVSTADEGRPQLFVRWLEVGQTTRLAELVEAPGALAWSNDGGSIAFTMFAPDEGAKL
                     GEAPPKPEGAEWAKPLEVITDITYRTDEGGYLKAGYTHVYVVAADGGAPRQLTFGAYN
                     EAGPLAWSADGRFVYAVGNRTPNWRREPVDTELYRITLADGSVTAITSRVGPDAAPDV
                     SPDGTKIAYLGYDDELLGFENGLVYVADPDGRNSRALTTALDRNVDAVAWADNRTLVI
                     KYDDLGSAKLARLDLTGRIEPLAEGLTGAGLDRPYTGGEFSVASNGNVAFTSGTAQRP
                     ADVAAVVRGRRVQLTHLNDAFLGGKSLGAVRSLPVTSSFDGRPVGAWLVTPPDFEAGK
                     KYPLILEIHGGPYAAYGPTFATDMQLYASAGYVVLYTNPRGSTSYGEEFANLIQHNYP
                     SQDYDDLMSAVDAAIATGYVDADKLYVTGGSGGGILTAWIVGKTNRFKAAAAQKPVIN
                     WASTVLTTDVATYMPKYWFAKPPWEDPDSYWSRSPLSLVGSVTTPTLVVVGDRDFRTP
                     VSDSEQYYQALQLKGVPTALVKVPDASHGGLAARPSQSASRVSAILAWFGQHTAAH"
     gene            6260..6886
                     /locus_tag="VM692_13155"
     CDS             6260..6886
                     /locus_tag="VM692_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HUQ53166.1"
                     /translation="MSSMRPQRIWPVAAFTGLYLVAATVGALAIGNREFLFYIVVMVL
                     LVGGVWLVHRGVELSTATLWALSIWGLAHMAGGLIAVPESWPINGDIRVLYSLWLIPQ
                     RLKYDQVVHAYGFGVATWVCWQGLRAAIGQRGGDGAPTFGLMVLAATAGMGLGALNEV
                     VEFAATLLVPETNVGGYLNTGWDLVANATGATLAALLIWLRGSRYAEG"
     gene            complement(6892..8262)
                     /locus_tag="VM692_13160"
     CDS             complement(6892..8262)
                     /locus_tag="VM692_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HUQ53167.1"
                     /translation="MSYENLEQALAAAKSPVEMLRNSQIGPYAFPVVPSEFTNWRDEQ
                     RSWRETCALFDQSHHMTDLYLHGPDALKLITALGVNTFNNFRVDTAKQFVACNHDGYV
                     IGDAILFHLAPDSFVFVGRPPALNWVQYNLETGNYNAVAERDERSFVNKTGRRVYRYQ
                     VQGPNAKKVMEKVTGKSVPDIKFFNMDVFDIAGCKVRALRHGMVGQPGWEIFGPWADG
                     DRVRDAIVEAGREFGIRKVGARTYPTSTLESGWIPSPLPAVYSGEPMKPYRQWLKGTS
                     YEATASLGGSFVSKNIESYYLTPYDMGYGGFVRFDHEFVGRAALEKIAKKPKKQKVTL
                     VWNGDDVDKVFSRLFHAGKFSKYIDLPLANYSTLPFDKIVKGGKTVGVSTYTGYSFNE
                     RAMLSLACVDVKYAKPGTKVSVVWGEDGGGTKKPTVERHNQATIRATVQPAPYGETAR
                     VAYRPK"
     gene            complement(8362..9765)
                     /locus_tag="VM692_13165"
     CDS             complement(8362..9765)
                     /locus_tag="VM692_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010139847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HUQ53168.1"
                     /translation="MAAHNLSAVLKAHGNAVKLLRNSKIGMYIYPVVAPEFSNWRNEQ
                     VAWRKSAVLFDQSHHMDELIVEGPDSAAFLEYVGINSFANFDLNRAKHFVPVTPAGYV
                     IGDMIIFREREDKFVLVGRAPTANWVRFQQAVGKWKVRLTHDPRSDSRPDGRAIYRTH
                     YRFQIPGPEANKIFDKINGGPVPDVKFFHVDWINIGSRRVQALRHGMAGAPGLEVWGP
                     YADKHYIQSTILQAARDVGVDLHLVGSRAYSTNTLESGWIPSPLPAIYTGGGMLQDYR
                     DWLGADSYEAVGSIGGSLETDNIEDYYVNPFELGYGFYIGWKTDFIGKDALTKMKDQK
                     NRKKVTLEWNRDDLLKVVASSFEEGVPYKWIDFPQPNYASTNADVLKNGNKQAGISMF
                     NGYSFNERCMLSLGVVNDDVEIGDQLTLIWGEPQTTGKTSTEPHRQAEIRVRVSPTPY
                     AREARENYAASWRTKSV"
     gene            complement(9854..11254)
                     /locus_tag="VM692_13170"
     CDS             complement(9854..11254)
                     /locus_tag="VM692_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HUQ53169.1"
                     /translation="MAHKNLEDVLKAAKNPVDMLRNSRIGAYVYPVVPGEFSNWRDEQ
                     AAWRKSCVLFDQSHHMVDIYIKGPDAIKLLSDLAINSFANFPVDRAKQFIPCSYSGHV
                     IGDGILFHLEKNNLVFVGRAPSANWIQYHAETGKYRVKIKKDDRSPGNPKGKAVNRIS
                     YRFQIQGPNAWQLIEKLNKKKVPELKFFHMSTMKIGGQKVRTLRHGMAGAPGLEIWGP
                     YKDREKIRETIIQAGEEFGLYQVGSRAYATNTLESGWIPSPLPAVYTDERMKKYRKWL
                     PANSYEATGSLAGSFVSDNIEDYYVTPYEIGYGPFVKFDHEFIGREALEKMADKPHRR
                     KVTFAWNGDDVTKVFQSMVMGEPDHYKYIDLPLSNYASSSFDKILDKNGNVVGASMFA
                     GYSYNEKSMLSLGIVDPKVELGDEVTLVWGEPNGGTRKTTVERHKQLNIRATVSAVPY
                     SKFVRETYASGWRTGT"
BASE COUNT         1790 a         3715 c         3753 g         2147 t
ORIGIN      
        1 tgcaggactt gatccgcaag atggccggcg tcgtttcgac gcgcgtcggc tacagcggcg
       61 gcgacgtcgc caacgcgacc tatcgccgtc acggcacgca cgccgaggcc atcgaggtcg
      121 tgttcgatcc gcagcgcctc acgtaccgca aactgctcga gtttttcttt cagatccacg
      181 atccgacgac gaagaatcgt cagggcaacg acatgggcac gtcgtatcgc tccggcatct
      241 actacctgac cgacgagcag cgcgagatcg ccgaggacac gatcgccgac gtcgatgcgt
      301 cgaagctctg gcccggcctc gtcgtgaccg aggtcaagcc ggccgggccg ttctgggagg
      361 ccgagcccga gcaccaggac tatttgctgc gttatccggg cggctacaca tgccacttcc
      421 cgcggccgaa ttgggtgttg ccgcggcgcg ccgcgcagcg gacggcgtga cggcatgagc
      481 acgcttgaag cacccgagcc gagctcgctg cgctggaaga gcgtcgtcga aatcgcgcgc
      541 gccggtaacg agccgccgcc gcgccgcgtc gagaagagcg acgccgaatg gcgccagcag
      601 ctttcggccg agcaatacca cgtcgcgcgc ggcaaaggca cggagcggcc gttcagctcg
      661 gagatgtgcg cgctgttcga gccggggcgc tatcactgca tctgttgcga cacgccgctg
      721 ttcgactcga cgacgaagtt cgacagcggc acgggctggc cgagcttcac gcaaccgctg
      781 acgcgcggcg tcgttgccta ccacctcgat acgagctacg gcatgcagcg cgtcgaggcg
      841 ctctgcaacg tctgcgacgc gcacttgggg cacgtgttcc cggacgggcc gccgccgagc
      901 ggcctgcggt tctgcatcaa cgccgtgtcg ctgcgcaggg ccaaagagtc gaagtagctc
      961 gtcacgccgg cccgcgcagc cgagcgcggt gctcgcggag caatactcgc gcggcgcagc
     1021 ctagcgcggc gcgtcagagc ggcggcgagt cgtcgccgag catcgcgcgg cgcacgagcg
     1081 ggatcggcgg gccgccatag ctcaagaacg tgtcgtgaaa tgatttccac gcctgtcgtc
     1141 cgccacggct ggcggtccag tcgtcgcgca gcttgcggat catgagcttg ccgagcgtgt
     1201 agttcagata cgccgggtcg taagtcccgc gtgccgcttg ctggcgcgag ttgccttcgt
     1261 cctgaaagca ctcgttgcga aagagctcgt acgactgctg ctgcgtcatg ccgcgcgtgt
     1321 gcaagccgat cgccgacaca taacggcagt tgcgcagcag cgcattcgac agctggccga
     1381 tgtgcgactc cgcgttgccg gcctcgaagc ccgcttccca aacgagctcc tcggcgtaat
     1441 ggccccagcc ttcggcggcc gcgtagctgt agaacacacg agcgatgggc aacgacgcgc
     1501 gattcgagtg caggaaattc aggaagtggc ccggccagac ctcgtgcacg gaggtgaaca
     1561 gcaggtcctt gctgcccatg acgtattcga gttgctcctc ggccgtccag ctcggatcgg
     1621 gcggcgcgat gtagtacacg gacggcagtc ccttctcgta ggggcccgga atgtcgatat
     1681 aggcggcgtt ttgccggttg tatggcggcg cttcttcgac gagcgcttcc tcgcttcccg
     1741 gtatcgacac gaggtccgtg tctaacagga actgcttcag tgccggcagc tgcgtgcgcg
     1801 cttcggcgac ggggccgagc ggcgatttcg tggcggccat cttcgccatg cagtcgccga
     1861 gcgtcgcccc gggagcgaac gtcgcacagg cttcgcgcag cgcgtcttgg ttgcgacgca
     1921 gatcggcccg gcccaacgcc tcgagcgcgt cgagcggcac cgtcacgtcc tcggtcgccg
     1981 cgagcatgcg cgcgaagcgt tcggcaccga gcgcgaaatc ctgcgttgcc atggcgcgtt
     2041 gggtttccag ccacgccgct gcggccgtca tcgcggttgc agcggtttcg gcggcggccg
     2101 cgagctcggc ctgcagtgcc gtgtcgccga cggaggcgaa cgcggctgtg ccgtcgccgc
     2161 ggtagtactc ggcgtagccg ttgaatccgg ctacgccgaa ctcgacgaac gacagcggca
     2221 gcggcgtgcg caagttcgcg cgaatttgcg cgagcgcgac cggcaccgcg cgcgcgtggg
     2281 cgacgaaggc gcgcagcctc gtcgctgcgt cggcataggg gcgcgtcgtg tagacgccgg
     2341 ggtcgagccc attgtcgaag taccagctca cgttggcatg aggctggtcg gcgtcctcga
     2401 gccaaaacag ctcgccgcgc gcgacgctcg cgagatagtc gcgctcgaag ctctgcgccg
     2461 cggagagctg cgtcgtgtcg aacgcttcgg cgtccgcgat cgcggcccgc aggaagtcca
     2521 tctgccgctg caagcttgcg gtgctccaat cgctgatccg gccgtcgaac tcgtgccgcc
     2581 cctgatagac ggcatagctc gggttcagct cgaaaaagcc ctcgatgaag cggtcgcgaa
     2641 aggcatccca agtggccgaa gccgccggcg cggccgccgg cgtaggaacg ccatcggagc
     2701 ccccgccatc ggagcgcccg caacccgccg cgagcaaaac cgctgccgcg agcgggatag
     2761 tcagcgaacg tagtttcatg gcgcgatcac agcacggccc ggtgtttgcg taaactgctc
     2821 tccgatggcc gtttcgccgc ccgccgcccc gactctgagc cgcacgctgc gctcgcggca
     2881 cgtcgcgctg atctcgatcg gcggcatcat cggcgcgggc ttgttcgtcg gcagcagcgc
     2941 caacatcgcg tccgtcggcc ccgccgtgct cgtgagctat tgcgtcgccg gcgggctgat
     3001 gttcctcgtc atgcgcatgc tggccgagat ggcggccgcg aacccggcgg cgctgtcgtt
     3061 cgccgaccac gcgcgtgccg ggctcggcgc ttgggccggg ttcgcctgcg gctggctcta
     3121 ttggtatttc tgggtcgtcg tcgtcggcat cgaggccatc gccggtgccg cgattctgca
     3181 aacgtggatc cccgcgcaag tttggcagat cggtctagtc ctcatggtgt tgctgacggc
     3241 gacgaacttg ctctcgacgc gctcgtacgg cgagttcgaa ttctggtttg cgtcgatcaa
     3301 ggtcgcggcg atcgtcgcgt tcatcctgat cggcggtagc tacgcgctcg gtttcagctc
     3361 gcccacgggc gcgacgttcg gcaatctcgt cgagcacggc ggcttcgcgc cgttcggcgc
     3421 cgcggccgtg ctcgcgggag tcacgggcat cgtgttctcg ctgacgggcg ccgagatcgt
     3481 gaccgtagtc gccgccgagt cgtccgagcc gtcgcaggcc atcgcagcgc tgacgacttc
     3541 gctgatcgtg cggattctcc tgttctacgt aggctccatg ctcgtcattc tcgctgtcgt
     3601 gccgtggacc gagattgccc ccgggtcttc gccgttcgct ctggcgctcg agcgcatgga
     3661 gattccgttc gcagcgacga cgatgaactt gatcgtgctc gtggccgtgc tgtcgtgctt
     3721 gaactcgggc ctctacgtca catcgcgcat gctgttcggc ctcgcggcgc agggcgacgc
     3781 gccgcgctcg ttggtcgcgc tcgatgcgcg cggcgtgccg acgcgcgcga ttctgatcgg
     3841 gacggtgttc ggctatctcg cggtattggc gtcggtcgcg tcgccgcagc gcctgttcac
     3901 gttcctcgtc aacgcttccg gcgccatcat gctcgtgatc tatctgctga tcgcgttggc
     3961 gcagattcgc cgccgtcgcg tcatggagcg cgatgcgcca gctcggctcg tggttcgcat
     4021 gtggctctat ccgtggctca gctacgtcac ggtcgccggg atcgggctcg tgctggcatc
     4081 gatgctgttt cgcacagccg agcgcgcgca gctcgccgcg agcgctctgt cggttgccgt
     4141 cgtgctggcg attttctggt ggaagcgccg ccgcgcttcg tcgaacagcg aacaggagta
     4201 gcgtccgatg cgaacgtctc tgtcagcgct cgtgctcgtc ttcgcggcga ccgcggcgag
     4261 cgcagcgccg cccgaagcgc cggcgcgtca gctcacgggc ctcgatctgt tcaatctgca
     4321 ggtggctacg gatccgcaga ttcgccccga cggtgccgtc gtcgcttaca cgcgcgtctc
     4381 gtacgacgtg atgaccgaca gcgctaggcc gtcgatctgg ctcatcgacg tcgcgacggg
     4441 acagcagacg ccgctcgtca ccggctccgg ctcgcactcg acgccgcgct gggcgcccgc
     4501 cggcaagcgc ctcgcctacg tttcgacggc cgacgaggga cggccgcagc tgttcgtgcg
     4561 ttggctcgag gtggggcaaa cgacgcgctt ggccgagctc gtcgaggcgc ccggcgcgct
     4621 cgcgtggtcg aacgacggag gctcgatcgc cttcacgatg ttcgcgcccg acgagggcgc
     4681 caagctcggc gaagcgccgc cgaagcccga gggcgccgag tgggcgaagc cgctcgaggt
     4741 catcaccgac atcacctatc gaaccgacga aggcggctat ttgaaggccg gctacacgca
     4801 cgtctatgtc gtcgcggccg atggcggcgc gccgcggcag ctgacgttcg gcgcttacaa
     4861 cgaagccggg ccgcttgcgt ggtccgccga cggccggttc gtctatgccg tcggcaatcg
     4921 cacgccgaat tggcgccgcg agcccgtcga caccgagctc tatcgcatta cgctcgccga
     4981 cgggtccgtg acggccataa cgagccgcgt cggtcccgac gcggcgcccg acgtgtcccc
     5041 cgacggaacg aaaatcgcgt atctcggtta cgacgacgag ctgctcggtt tcgagaacgg
     5101 tctcgtctac gtcgcggatc ccgacgggcg caattcgcgc gcgctcacga ccgcactcga
     5161 tcgcaacgtc gatgccgtcg cgtgggccga caatcgcacg ctcgtcatca aatacgacga
     5221 cttaggatca gcgaagctcg cgcggctcga tctgacaggc cgcatcgagc cgctggccga
     5281 gggtttgacg ggcgcgggtc tcgatcggcc gtacacgggc ggcgagttct cggtcgcgag
     5341 caacggcaac gtcgcgttca cgagcggcac ggcgcagcgg ccggcggacg tcgcggccgt
     5401 cgtgcgcggg cgccgcgtgc agctcacgca cctcaacgac gcgttcttgg gcggtaagtc
     5461 gctcggcgcg gtgcgaagcc tgcccgtcac gtcgagcttc gacggtcgcc ccgtcggcgc
     5521 ctggctcgtc acgccgcccg acttcgaggc cggcaagaag tatccattga tcctcgagat
     5581 ccacggcggg ccttatgccg cctatggccc gacgtttgcg accgacatgc agctctacgc
     5641 gagcgccggc tacgtcgtgt tgtacacgaa cccgcgaggc tcgacgtcgt acggcgaaga
     5701 gttcgcgaac ctgatccagc acaactatcc gagccaggac tacgacgacc tcatgagcgc
     5761 cgtggatgcg gcgattgcga cgggttacgt cgacgccgac aagctgtacg tcacgggagg
     5821 gtccggcggc ggaatcttga cggcgtggat cgtcggcaag acgaaccgct tcaaagctgc
     5881 ggcggcgcag aagcccgtca tcaattgggc cagcacggtg ctgacgaccg acgtcgccac
     5941 gtacatgccg aaatactggt tcgcgaagcc gccgtgggaa gaccccgaca gctactggag
     6001 ccgctcgccg ttgtcgctcg tgggctcggt gacgacgccg acgctcgtcg tcgtcggcga
     6061 ccgcgacttc cgcacgcccg tcagcgattc cgagcagtac taccaggcgt tgcagctaaa
     6121 gggcgtgccg acggcgctcg tcaaagtgcc cgacgcttcg cacggcggct tggccgcgcg
     6181 cccgtcgcag tcggcgtcgc gcgtcagcgc gatcctagcc tggttcggcc agcacacggc
     6241 cgcgcactga gccgagcaga tgtcgagcat gcggccgcag cgcatctggc ccgtcgccgc
     6301 gttcacgggg ctgtatctcg tagccgcaac cgtcggcgca cttgcgatcg gtaatcggga
     6361 attcctcttc tacatcgttg tcatggtgct gctggtcggc ggcgtctggc tcgtgcatcg
     6421 cggcgtcgag ctttcgacgg cgacgctgtg ggcgctcagc atctggggtc tcgcgcacat
     6481 ggcgggcggc ttgatcgccg tgccggagtc gtggccgatc aacggcgaca tccgcgtgct
     6541 gtactcgctc tggctgattc cgcagcgcct gaagtacgac caagtcgtgc atgcctacgg
     6601 gttcggcgtc gcgacctggg tctgctggca ggggttgcgc gccgccattg gacagcgcgg
     6661 cggcgatggc gcgccgacgt tcggcctcat ggtgctcgca gccaccgccg gcatggggct
     6721 cggcgcgttg aacgaggtcg tagagttcgc cgcgacgttg ctcgtgccgg agacgaacgt
     6781 aggcggttat ctgaacaccg gttgggattt ggtcgccaac gccacaggcg cgacgcttgc
     6841 cgcgctgctc atctggctgc gcggcagtcg ctacgccgaa ggttgatacg cctatttcgg
     6901 tcggtaggcg acccgcgccg tctcgccgta cggcgccggt tgcacggtcg cgcggatcgt
     6961 cgcctgattg tggcgctcga ccgtcggctt cttagtgccg ccgccgtctt cgccccagac
     7021 gaccgacacc ttcgtgccgg gcttggcgta cttcacgtcg acgcaggcca aagacagcat
     7081 ggcgcgctcg ttgaagctgt agcccgtgta cgtcgatacg ccgacggtct tgccgccctt
     7141 gacgatcttg tcgaacggca gcgtcgagta gttcgcgagc ggcaagtcga tgtacttcga
     7201 gaacttaccc gcgtggaaca gccggctgaa aaccttgtcg acgtcgtcgc cgttccagac
     7261 gagggtcacc ttctgcttct tcggcttctt ggcgattttc tcgagcgccg cgcggccgac
     7321 gaactcgtga tcgaaacgaa cgaagccgcc gtaacccatg tcgtacggcg tcaagtagta
     7381 gctctcgatg ttcttcgaga cgaagctgcc gccgagcgag gccgtcgcct cgtagctcgt
     7441 gcccttgagc cactggcgat acggcttcat gggctcgccg gagtagacgg ccggcaacgg
     7501 tgacgggatc caaccggact cgagcgtgct cgtcgggtag gtgcgggcgc cgactttacg
     7561 gatgccgaac tcgcgcccgg cctcgacgat cgcgtcgcgc actctgtcgc cgtcggccca
     7621 ggggccgaag atctcccagc cgggttggcc gaccatgccg tggcgcagcg cgcggacctt
     7681 gcagccggcg atgtcgaaca cgtccatgtt gaagaacttg atgtcgggca cggacttgcc
     7741 cgtgaccttc tccatgacct tcttcgcgtt cgggccctgg acttgatagc gataaacgcg
     7801 ccggcccgtc ttgttgacga acgaacgctc gtcgcgctcg gcaacggcgt tgtagttgcc
     7861 cgtctcgagg ttgtattgaa cccaattgag cgccggcggc cggccgacga acacgaagct
     7921 gtccggcgca agatggaaca ggatcgcgtc gccgatgacg tagccgtcgt ggttgcaagc
     7981 gacgaattgc ttcgccgtgt ccacgcgaaa gttgttgaag gtgttgacgc cgagcgccgt
     8041 gatcagcttc agcgcatcgg gaccgtgcag gtacaggtcc gtcatgtgat gcgactgatc
     8101 gaacagcgcg caggtctcgc gccacgaccg ctgctcgtcg cgccagttcg tgaactcgct
     8161 cgggaccacg gggaacgcgt acggaccgat ctgggaattg cgcagcatct ctacggggct
     8221 cttcgcggcc gccaatgctt gttctaagtt ctcgtagctc atagaccctg tttaagcccc
     8281 cttacagacc tagtgcttcc caacctgtta ggccgggcac cctaaagcaa caatcgtctc
     8341 aacgctactt cgcgctcgct ctcagacgct cttcgtgcgc cagctcgcgg cgtagttctc
     8401 ccgagcttcc cgcgcatacg gcgtcggcga cacgcgaacg cggatttcgg cctgccggtg
     8461 cggctcggtc gatgttttac ccgtcgtctg cggctcgccc cagatcagcg tcagctggtc
     8521 gccgatctca acgtcgtcgt tgacgacgcc gagcgagagc atgcagcgct cgttgaacga
     8581 atagccgttg aacatcgaga tgccggcttg cttgttgccg ttcttgagca cgtccgcgtt
     8641 cgtcgacgcg tagttcggct gcgggaagtc gatccacttg tacggcacgc cttcctcgaa
     8701 cgaggaggcc acgaccttca agaggtcgtc gcgattccac tcgagcgtga ctttcttgcg
     8761 gttcttctgg tccttcatct tcgtcagcgc gtctttgccg atgaagtcgg tcttccaacc
     8821 gatatagaag ccgtagccga gctcgaacgg attgacgtag taatcctcga tgttgtcggt
     8881 ctcgaggctg ccgccgatcg agccgacggc ctcatagctg tcggcgccga gccagtcgcg
     8941 gtagtcctgc agcatgccgc cgccggtgta gatcgcgggc agaggcgagg ggatccaacc
     9001 cgactcgagc gtgttcgtcg agtacgcgcg gctgccgacg aggtgcaggt ccacgccgac
     9061 gtcgcgcgcg gcctgcaaga tcgtgctctg aatgtagtgc ttgtcggcat agggtcccca
     9121 gacctcgagg cccggagcgc cggccatgcc gtggcgcagc gcctgcacgc gcctcgaccc
     9181 gatgttaatc cagtcgacgt ggaagaactt gacgtcgggg acggggccgc cgttgatctt
     9241 gtcgaagatc ttattcgcct cgggacctgg aatttggaac cggtagtgcg tgcggtagat
     9301 cgcccggccg tcgggccggc tgtcggagcg cgggtcgtgc gtgagccgca ccttccattt
     9361 gccgacggct tgctggaagc gcacccagtt cgccgtcggc gcacggccga cgagcacgaa
     9421 cttatcctcg cgctcgcgga aaatgatcat gtcgccgatg acgtagccgg ccggcgtcac
     9481 aggcacgaag tgcttggcgc ggttcaggtc gaagttcgcg aagctgttga tgccgacgta
     9541 ctcgaggaac gctgcggagt ccggcccttc gacgatcagc tcgtccatat gatggctctg
     9601 atcgaacagc accgccgact tacgccacgc aacctgctcg ttgcgccaat tcgagaactc
     9661 cggcgcgacg accggataga tgtacatgcc gatcttcgag ttgcggagca gcttgacggc
     9721 gttgccgtgc gccttcagca cagcgctcaa gttgtgtgcg gccatggttt ccctcgtgtc
     9781 cttcaggaaa aagggcgagc gtcgttgccg acggctcgcc caattcgagc ggagcccgaa
     9841 gctccgctct ccgttaggtg ccggtgcgcc agcccgacgc gtacgtctcg cgaacgaact
     9901 tcgagtacgg caccgcgctg acggtcgcgc gaatgttgag ctgcttgtgg cgctcgaccg
     9961 tcgtcttgcg tgtgccgccg ttgggctcgc cccaaacgag cgtcacttcg tcgccgagct
    10021 cgaccttagg gtcgacgatg ccgagcgaca gcatcgactt ctcgttgtag ctgtagcccg
    10081 cgaacatcga tgcgccgacg acgttgccgt tcttgtcgag gatcttgtcg aacgacgacg
    10141 acgcgtagtt cgacaacggc aggtcgatgt acttgtagtg atcgggctcg cccatgacca
    10201 tcgactggaa caccttggtg acgtcgtcgc cgttccacgc gaacgtcacc ttgcggcgat
    10261 gcggcttgtc ggccattttt tcgagcgcct cgcgcccgat gaactcgtga tcgaacttca
    10321 cgaacgggcc gtagccgatc tcgtacggag tgacgtagta gtcctcgatg ttgtccgaga
    10381 cgaagctgcc ggcgaggctg cccgtcgcct cgtagctgtt ggccggcagc cacttgcggt
    10441 acttcttcat gcgctcgtcc gtgtagacgg ccggcaacgg cgacggaatc cagcccgact
    10501 cgagcgtgtt cgtcgcgtaa gcgcgcgagc cgacttgata caggccgaac tcttcgccgg
    10561 cctggatgat cgtctcgcgg atcttttcgc ggtctttgta cgggccccag atctcgagac
    10621 ccggggcgcc ggccatgccg tggcgcagcg tgcgcacctt ctggccgccg atcttcatcg
    10681 tgctcatgtg gaagaacttg agctcgggga ctttcttctt gttcagcttc tcgatcagct
    10741 gccacgcgtt cgggccttgg atctggaagc gatacgagat gcggttgacg gccttgccct
    10801 tcgggttgcc gggcgagcgg tcgtccttct tgatcttgac gcggtacttg cccgtctcgg
    10861 cgtggtactg aatccagttc gcgctcggtg cgcgaccgac gaacacgagg ttgttcttct
    10921 cgaggtggaa cagaatgccg tcgccgatga cgtggccgct gtagctgcac ggaatgaact
    10981 gcttcgcgcg gtcgacgggg aagttagcaa agctattgat cgcgagatcc gacagcagct
    11041 tgatggcgtc cggacccttg atgtagatgt cgaccatgtg gtgcgactga tcgaaaagca
    11101 cgcagctctt gcgccacgca gcctgctcgt cgcgccagtt cgagaactcg ccgggcacca
    11161 cggggtagac gtaagccccg atgcgcgaat tgcggagcat gtcgacgggg ttcttggcgg
    11221 ctttcagcac gtcctcaaga tttttatgag ccatggcttc gggtgccctc ctaaggcgaa
    11281 aagctctatc cggctttttc gagggcggct agtctagcac cagaaccccg tggttcggct
    11341 atggtgctcg gctttagttg ctcgccactc cgcgaaacta ccgtttcgca aaattactgc
    11401 gtagt
//
LOCUS       DATLEE010000605         2575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4426968, whole genome shotgun sequence.
ACCESSION   DATLEE010000605 DATLEE010000000
VERSION     DATLEE010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4426968"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VM692_13175"
     CDS             <1..743
                     /locus_tag="VM692_13175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HUQ53170.1"
                     /translation="AQRLEVDLAIVTPTGPNDTITSKDVERVAKILKEVGPLEMLRGV
                     RRTMARSMAQAHAEVAPVTVVDDADLHAWWGGKDTLMLRLIRAIAAGVKAEPALNAWY
                     DSRAIGRRLLKSIHLGVAVDSPDGLFVPVLRDVGARTPEDLHEGLERLRRDVRARTIP
                     PEELRGYTFILSNFGTFGGRYANPIVQPPTVAILGAGRVRKEVVAHDGQPAVHPVLPL
                     SLTFDHRAVTGGEATRFVAQVIADLELG"
     gene            755..1933
                     /locus_tag="VM692_13180"
     CDS             755..1933
                     /locus_tag="VM692_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUQ53171.1"
                     /translation="MVNLDFDFGLGEDLALLREQARRFAMERVAPLAARVDETDQFPR
                     ELWPEIGELGLFGIAVDEALGGAGLGYLAEVVAVEELSRASAAVGLSYGVHAQLCVNQ
                     IARRGTPEQHARFLPKLLSGEHVGALAMSEVNAGSDVLGMRTTAVPDGDGYILDGTKM
                     WITNGPQADVLIVYARLADGQKLNGHGPTIKAFIVERTLPGFAADPKLDKLGMRGSET
                     SGLVFNQCRIPASHVLPGDGKEILMSGLDYERVVLAGGALGIMRACLDVTLPYIHARK
                     QFDRPIGAFELMQGKLADMYTMMNSSRAFVYAVAAACDRGQRTRFDAAAAILKASEGA
                     TWMAGQTIQALGGNGYTTEYPAGRLGRDAKLYEIGAGTNEIRRMLIGRELFAATAEAH
                     "
     gene            1933..>2575
                     /locus_tag="VM692_13185"
     CDS             1933..>2575
                     /locus_tag="VM692_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HUQ53172.1"
                     /translation="MGVFESKIDSSSEEFRKNAAANRALAERLRKLAAEVETGGSERA
                     RARHRERGKLPVRARVAKLLDPNTPWLEIGKFAAHEVYDSALPAAGLVCGIGIVRGTR
                     CMVVANDATVKGGTYYPLTVKKHLRAQEIALENRLPCVYLVDSGGAFLPMQDEIFPDR
                     EHFGRIFFNQAQMSARGIPQIAVVMGSCTAGGAYVPAMSDETVIVRGTGTIFIG"
BASE COUNT          420 a          877 c          875 g          403 t
ORIGIN      
        1 tcgcgcaacg cctcgaggtc gatctcgcga tcgtcacgcc gacgggccca aacgacacga
       61 tcacgtcgaa ggacgtggag cgcgtcgcga agattttgaa agaagtcggc ccgctcgaaa
      121 tgctgcgcgg cgtacgccgc acgatggcgc gcagcatggc gcaagcgcac gccgaggtcg
      181 cgcccgtcac cgtcgtcgac gacgccgacc tgcacgcctg gtggggcggc aaggacacgc
      241 tgatgctgcg cttgatccgc gcgatcgcgg ccggcgtcaa agccgagccg gcgttgaacg
      301 cgtggtacga cagccgcgcg atcggccggc gtctcttgaa aagcatccat ctcggcgtcg
      361 ccgtcgacag ccccgacggc ctattcgtgc cggtgctgcg cgacgtcggc gcgcgcacac
      421 cggaagattt gcacgagggt ctcgagcgcc tgcggcgcga tgttcgtgcg cgcacgattc
      481 ctcccgagga gctgcgcggc tacacgttca tcctgtcgaa cttcggcaca ttcggcggcc
      541 gctatgcgaa cccgatcgtg cagccgccaa cggtcgcgat cctcggcgcc ggccgcgtgc
      601 gcaaggaggt cgtcgctcac gacggccaac ccgccgtgca tcccgtcttg ccgttgtcgc
      661 tgacgttcga tcatcgcgcc gtcacgggcg gcgaagcgac gcggttcgtg gctcaagtca
      721 tcgccgacct cgagctcggc taacgaggcg acctatggtc aatctcgact tcgatttcgg
      781 tcttggggag gatctcgcgc tgctgcgcga gcaggcgcgg cgcttcgcga tggaacgcgt
      841 cgcgccgctg gccgcccggg tcgacgaaac ggaccagttc cctcgcgagc tctggccgga
      901 gatcggcgag ctcggcttgt tcggcattgc cgtcgacgaa gcgctcggcg gagcgggtct
      961 cggctacctc gccgaggtcg tcgcggtcga ggaattgagc cgtgcctccg cggccgtggg
     1021 gctctcgtac ggcgtgcacg cgcagctctg cgtgaaccag atcgcgcggc gcggcacacc
     1081 cgagcaacac gcgcgcttcc tgccgaagct cttgagcggc gagcatgtcg gcgcgctcgc
     1141 gatgagcgag gtcaacgccg gctccgacgt gcttggcatg cgcacgaccg ccgtgcccga
     1201 cggcgacggc tacatcctcg acggcacgaa gatgtggatc acgaacggcc cgcaagcgga
     1261 cgtgctgatc gtctacgcgc gcctcgccga tggccagaag ctgaacggcc acggccccac
     1321 aatcaaagcg ttcatcgtcg agcggacgct gccgggcttc gccgccgatc cgaagctcga
     1381 caagctcggc atgcgcggct ccgagacatc gggcctcgtg ttcaaccagt gccgaattcc
     1441 ggcaagccac gtcttgccgg gcgacggcaa agagatcctg atgtcggggc tcgactacga
     1501 gcgcgtcgtg ctcgccgggg gagcgctggg catcatgcgc gcctgcctcg acgtcacgct
     1561 gccgtacatc catgcgcgca agcagttcga tcggccgatc ggcgcgttcg agctcatgca
     1621 aggcaagctc gcggacatgt acacgatgat gaattcgtcg cgcgcgttcg tgtacgccgt
     1681 cgccgcggct tgcgaccgcg gccaacgcac gcgcttcgac gccgccgctg cgatcctcaa
     1741 agcgtccgag ggcgcgacat ggatggccgg ccagacgatt caagcgctcg gcggcaacgg
     1801 ctacacgacc gagtatccgg ccggccgcct cgggcgcgac gcgaagctct atgagatcgg
     1861 cgccggcacg aacgagatcc gccggatgct gatcggccgg gagctgttcg cggcgaccgc
     1921 cgaggcgcac tgatgggtgt cttcgaaagc aagatcgact cgagtagcga ggagttccgc
     1981 aagaacgccg cggcgaaccg cgcgctcgcc gagcggctgc gcaagctcgc ggccgaggtc
     2041 gagaccggcg gctccgagcg tgcccgcgcg cgccaccgcg agcgcggcaa gctgcccgtg
     2101 cgggctcgcg tcgcgaagct gctcgacccg aacacgccgt ggctcgagat cggcaaattc
     2161 gccgcgcacg aggtctacga ctccgcgctg ccggccgcgg gcttggtctg cggcatcggc
     2221 atcgtgcgcg gcacgcgctg catggttgtc gcgaatgacg cgaccgtcaa aggcggcacg
     2281 tactatccgt tgacggtcaa gaagcacctg cgtgcccaag agatcgcgct cgagaatcgc
     2341 ctgccgtgcg tctatctcgt tgattcgggc ggcgccttct tgccgatgca ggacgagatc
     2401 ttccccgacc gcgagcactt cgggcggatc tttttcaacc aagcgcagat gtcggcgcgc
     2461 ggcattccgc agatcgccgt cgtcatgggc tcgtgcacag cgggcggcgc ctacgttccc
     2521 gcgatgtcgg acgagacggt catcgtccgt gggaccggaa cgatcttcat cggcg
//
LOCUS       DATLEE010000606         2356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5196428, whole genome shotgun sequence.
ACCESSION   DATLEE010000606 DATLEE010000000
VERSION     DATLEE010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5196428"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VM692_13190"
     CDS             <1..1338
                     /locus_tag="VM692_13190"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ53173.1"
                     /translation="KKHLSRRTLLKGAGVALGLPLLDSMFPAATAFAQAAAAPPRRFF
                     AGFVPHGAAPGYWVPEKEGALPTKLPFMWEPLEPVRKSVTILTGLHSTSSEPPPGETG
                     ADHWVAAAFLCADKPKKTAGADVRAGHTIDQMIAEKIGRETLLPSVEMSVEDPGSGSS
                     NCGEGYSCVYTNTIAWASPTTPLPMELNPQVVFERMFGSGSTPEQRVARRERNQSILD
                     SINGKIAGLRQEISVPDRTRLDSFTQNVREIERRLEIAAKATTAAPEDFAVPPGIPQS
                     FDEHIKLMFDLLALAYQADLTRVGTLLFARDLTGRRYPESAAPNAGFHGVSHHGEDPA
                     LIKELSKINQYHVKMVAYFADKLSKIEEADGTLLDHSLLLYGSNMGNPNQHLHYDVPH
                     VLIGGNHGRMKGNRHLAYPTKTISTGNLLLSLLDQFDIEQDSIGDSTGRLTNL"
     gene            1335..>2356
                     /locus_tag="VM692_13195"
     CDS             1335..>2356
                     /locus_tag="VM692_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ53174.1"
                     /translation="MSRVSAIVQAIALALAVACASTALAQTPVADAAMRRDGAEVRRL
                     VQSGADVKAAQADGATALHWAAYYGDASLAALLLEKGADVAAVNRNGSTPLWLASSQG
                     DAAMIEALLKGGADANEALPLGRRPLMLAARSGRVDAVNALLAGGADVNAKEEARGTT
                     ALMQAADQGHADVLAVLLEHGADVAAVSSAVMRDGRTAALGKSDDPRSAVRRQVIAIL
                     CDQPTPDLGKLQELTGETEDAVLAEAAGGAGGNPCNLQRDNLGFVIAASGGRRGRAPR
                     EADGGELTALIYASRTGSIEAARVLLDAGADVNQVSRYGWSPLLAATQNRNYRLGKFL
                     IERGADV"
BASE COUNT          404 a          797 c          804 g          351 t
ORIGIN      
        1 aagaagcatc tttcgcgacg cactctgctg aagggcgcgg gcgtagcgct cggcttgccg
       61 ctgctcgact cgatgtttcc ggctgcgacg gcgttcgcgc aggcagccgc ggccccgccg
      121 cgcagattct tcgcaggctt cgtgccgcac ggcgcggcgc cgggctactg ggtgcccgag
      181 aaagagggcg cactgcccac gaagctgccg ttcatgtggg aaccgctcga gcccgtgcgc
      241 aagagcgtca cgattttgac gggcctgcac tcgacgtcgt cggagccgcc gcccggcgag
      301 acgggcgccg accactgggt cgccgcggcc ttcctctgcg ccgacaagcc gaagaagacc
      361 gccggcgccg acgtgcgcgc cggtcacacg atcgatcaga tgatcgccga gaagatcggc
      421 cgcgagacgc tgctgccttc cgtcgagatg tccgtcgaag atcccggctc cggctcgagc
      481 aactgcggcg agggttacag ctgcgtctac acgaacacga tcgcgtgggc gtcgccgacg
      541 acgccgctgc cgatggagtt gaacccgcag gtcgtgttcg agcgcatgtt cggcagcggc
      601 agcacgcccg agcaacgcgt cgcgcggcgc gagcgcaacc aaagcatcct cgactcgatc
      661 aacggcaaga tcgccggctt acgccaagag atcagcgtgc ccgatcggac gcggctcgac
      721 agcttcacgc agaacgtgcg cgagatcgag cgtcgtctcg agatcgcggc caaggccacg
      781 acggcggcgc ccgaggattt cgccgttccg cccggcattc cgcagtcgtt cgacgagcac
      841 atcaagctga tgttcgatct gctcgcgttg gcgtaccaag ccgacctcac gcgcgtcggc
      901 acactgttgt tcgcgcgcga tctcacgggc cggcgctatc ccgagagcgc ggcgccgaat
      961 gcgggcttcc acggcgtgtc gcatcacggc gaggacccgg cgttgatcaa ggagctttcg
     1021 aagatcaacc agtatcacgt caagatggtc gcgtactttg ccgacaagct ctcgaagatc
     1081 gaagaggccg acggcacgct gctcgatcac tcgctgctgc tttacggcag caacatgggt
     1141 aacccgaatc agcacttgca ctacgacgtg ccgcacgtgc tgatcggcgg caaccacggc
     1201 agaatgaagg gcaaccgcca tctggcgtac ccgacgaaaa cgatctcgac cggcaatttg
     1261 ctgctgagct tgctcgacca gttcgacatc gaacaagaca gcatcggcga cagcacggga
     1321 cgactgacga acctatgagc cgcgtctcgg ccattgtgca ggcaatcgcg ctggcgctcg
     1381 ccgttgcttg cgcgagcacg gcgctcgcgc agacgccggt ggccgatgcc gcgatgcgcc
     1441 gcgacggcgc cgaagtgcgc cggctcgtgc agagcggcgc cgacgtgaaa gccgcgcaag
     1501 ccgacggcgc cacggcgctg cactgggccg cttattacgg cgacgcgagc ttggctgcgc
     1561 tgttgctcga gaagggcgcc gacgtcgcgg ccgtgaaccg caacggctcg acgccgctgt
     1621 ggctcgcctc gagccaaggc gacgctgcga tgatcgaggc cctgctgaag ggcggcgccg
     1681 atgcgaacga agctctgccg ctcggccgca ggccgttgat gcttgcggcc cggtcgggac
     1741 gagtcgatgc cgtgaacgcg ctgctcgcgg gcggtgccga cgtgaatgcg aaagaggaag
     1801 cgcgcggcac gacggcgttg atgcaagcgg ccgaccaagg ccatgccgac gtgctcgcgg
     1861 tgctgctcga gcacggtgcc gacgtcgcgg cagtgtcttc tgcggtcatg cgcgacggcc
     1921 gcacggccgc gctcggcaag tccgacgatc cgcgctcggc tgtgcgccgg caggtcatcg
     1981 ccattctctg cgaccagccg acgcccgacc tcggcaagct gcaggagctc accggcgaga
     2041 ccgaggacgc cgtactggcg gaggctgcgg gcggcgccgg tggcaatccg tgcaacctgc
     2101 agcgcgacaa cttggggttc gtcatcgccg ccagcggcgg tcgacgtggg cgcgctccgc
     2161 gcgaggccga cggcggcgag cttacggcgc tgatctatgc gtcgcgcacg ggcagcatcg
     2221 aggccgcacg cgtgctgctc gatgccggcg ccgacgtcaa tcaagtttcc cgttacggct
     2281 ggagcccgct gctggccgcg acgcagaacc gcaactatcg cctcgggaag ttcctcatcg
     2341 agcgcggcgc cgacgt
//
LOCUS       DATLEE010000607         4588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8561537, whole genome shotgun sequence.
ACCESSION   DATLEE010000607 DATLEE010000000
VERSION     DATLEE010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8561537"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..677)
                     /locus_tag="VM692_13200"
     CDS             complement(114..677)
                     /locus_tag="VM692_13200"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HUQ53175.1"
                     /translation="MRTSVILVAALLAAPPVLAQDDPNTEGLYLGVGLGDFSTGIEDI
                     NDVDEANLDFDPDDNARKLFAGWRFNRFVALQLDFVDFQRSRDARNQLNVVSTQSDGI
                     APSIVGTLPLGPVELFARAGVLWYDLEINRGNTAVVDNSDRDPVFGAGLGVTVAKRLN
                     LRAEYEVVEIDGLDDSNAVWLSAAWRF"
     gene            complement(805..1713)
                     /locus_tag="VM692_13205"
     CDS             complement(805..1713)
                     /locus_tag="VM692_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUQ53176.1"
                     /translation="MSLRVIIAAPVALLIIAGCNSLPDRVDTVEQARESIAALEREPL
                     AGRVASREINAARDALAEADDAYEEGQSLEIIEHNAYIAQRYADISRELVAEASAREE
                     ITRGEAERNRIIAEARERDAQAAEREAEAANRELEVQSRAAEEQGRAAQAAEERSREL
                     ERELEDLEARNTEDRGLVLTLGDVLFDTGTATLKPGAATTVDRLAQFMRDYPERSVRI
                     EGHTDAAGSDETNQALSERRAQAVRDALVMRGLPAERIGTMGYGEARPIAGNDSPGGR
                     QQNRRVEIVVSDAQGAFAEDSRRKLL"
     gene            complement(1743..2159)
                     /locus_tag="VM692_13210"
     CDS             complement(1743..2159)
                     /locus_tag="VM692_13210"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HUQ53177.1"
                     /translation="MNRNQRVVMLFGLLLIGAVMLGCASSNRVETAASASYEDDLDRA
                     RDSLADAERAGAAEYGNAELALAREKLREAERAIAEGETARAQRLAVEADLDADVAVA
                     VNRNRETQQLVTEARSNLRTLEEELRRGQRDDSSAR"
     gene            2392..3246
                     /locus_tag="VM692_13215"
     CDS             2392..3246
                     /locus_tag="VM692_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53178.1"
                     /translation="MADPTENEGALKDAALKDAKNLAAAARDRGMEQLEGAKGQLAEG
                     AERVASAVERTANELDGDGDGAISGYGHSLANLMRQLAGGLRERDIEQFASELGSLAR
                     RNPGMFLAGSVALGFGVARFFKARAPQRRADDTYGGYAGGSGGNEWRDRSAQDGYDRG
                     EFDADESLDLSGNPTRVQRPDDDEANGAIGDSSTEQWARTETPASSTQDSSAARGDER
                     QAKSRSGKNKAKTQRASAGANPKPTEGPSSPLGDSSATDGSLTSGDGDSAFTGGTGGG
                     GLRGGKES"
     gene            3243..3698
                     /locus_tag="VM692_13220"
     CDS             3243..3698
                     /locus_tag="VM692_13220"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HUQ53179.1"
                     /translation="MSTRTSDMGSRERTGPRNIEARDYESTPTLLRRLLNDVGTLFAQ
                     EVALLKAETTESIGDLKAATVSMAMGGAVAFMGAFFLLLSAVYGLSNVVDPWLAALIV
                     GGVVTLIGVIMLVTGSKKLEPRAVAPRRTAAALRRDTEMVKGVAQHEHE"
     gene            3706..4560
                     /locus_tag="VM692_13225"
     CDS             3706..4560
                     /locus_tag="VM692_13225"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HUQ53180.1"
                     /translation="MKPTLDADSTRYAEHSDSDDRDPAALERDIDTTRADVRATLAAL
                     ERRLSFDRLVEMTVGRIRERGGEFAGNLTDTATQNPMPVLLTSIGLGWMMLMSRRGNG
                     ASQPSRARARAAEAADSVSGAAERFGERFSGAKDRFGEQVSGAAHGASERMHEAGSRL
                     HEAVESSRQTLGATAESMRDNASRMASATREQMSYATDRIDQLLHEQPLLLGALGLAA
                     GALIGALLPTSEAEGRLLGEARDKAVKNVAQKSRTLYETARDNAAQFAAPGGRDEDAS
                     SERPSRPH"
BASE COUNT          776 a         1480 c         1587 g          745 t
ORIGIN      
        1 aatcggcggc gtcgatgagg acggcggcgc gcgcagcgtg cgcgcgccgc caacacgtct
       61 cgccttcgat cagcagcgtg tcggctgcag tttcccggtc ggccgtggct gcgctagaac
      121 cgccaagctg ccgagagcca gaccgcgttg ctgtcatcga gcccgtcgat ttcgacgact
      181 tcatactccg ctcgcaggtt caggcgtttg gcaacggtga cgccgagacc ggccccgaac
      241 accgggtcgc ggtcgctgtt gtcgacaacg gccgtattgc cgcggttgat ctcgaggtcg
      301 taccaaagga cgccagcgcg cgcgaataac tcgacggggc cgagcggcag cgtgccgacg
      361 atgctgggcg cgatgccgtc ggactgcgtc gagacgacgt tgagctgatt gcgtgcgtcg
      421 cgcgaacgct ggaagtcgac gaagtcgagc tgcagggcga cgaaacggtt gaaccgccaa
      481 cctgcgaaca gcttgcgagc gttgtcatca ggatcgaagt cgagattcgc ttcgtcgacg
      541 tcgttgatgt cttcgatgcc cgtcgagaag tcgccgaggc cgacgccgag ataaagccct
      601 tcggtattgg ggtcgtcttg cgcgagaacc ggcggcgcgg cgagcagcgc cgcaacaaga
      661 atgactgacg ttcgcatgac tgactccttg tcgaaagccg gcccatccag ggccgcacag
      721 cgtcggctcg aaactccgaa ggaccggccc tgagctctag gggggaaaga gccgttgagg
      781 tgggccgatc ctccggaaag cacttcacag cagtttccgg cggctgtcct cggcgaaggc
      841 tccctgcgca tcggacacga cgatctcgac gcgccggttc tgctgccggc cgccggggct
      901 gtcgttgccg gcgatcggac gggcctcgcc gtagcccatc gtgccgatgc gctcggccgg
      961 cagtccgcgc ataacgagcg cgtcgcggac ggcttgcgcg cgccgctccg acagagcttg
     1021 attcgtctcg tcgctgccgg cggcatccgt gtgaccctcg atgcgaaccg accgctcggg
     1081 gtagtcgcgc atgaactgag cgagccggtc gaccgtcgtg gcggcgcctg gcttcagcgt
     1141 cgcggtgccc gtgtcgaaca agacgtcgcc cagcgtcagc accagtccgc gatcctccgt
     1201 gttgcgcgcc tcgaggtctt cgagctcgcg ctcgagctcg cggctgcgct cctcggcggc
     1261 ttgtgctgca cgtccctgct cttcggcggc gcgtgactgg acctcgagct cgcgattcgc
     1321 tgcctcggcc tcgcgttccg cggcttgtgc atcgcgctcg cgcgcttcgg cgatgatgcg
     1381 gttgcgctcg gcttcgccgc gcgtgatctc ctcgcgtgca cttgcctcgg caacgagctc
     1441 gcgcgaaatg tcggcgtagc gctgtgcgat gtaagcgttg tgctcgatga tctcgagcga
     1501 ctggccttct tcatacgcgt cgtcggcttc ggccaatgcg tcgcgcgccg cgttgatttc
     1561 gcgcgaggcg acgcggccgg cgagcggctc gcgttcgagg gcggcgatcg actcgcgcgc
     1621 ttgctcgacg gtatcgacgc ggtcgggcag gctgttgcaa cccgcgatga tcagcagcgc
     1681 gacaggcgct gcaataatta ctcgtagact catgactgaa ctctcctccg tgtgcttctc
     1741 cgctaacgcg cggaggaatc gtctctctgg ccgcggcgca gctcttcctc gagcgtccgc
     1801 aagttggacc gcgcttccgt tacgagctgt tgagtttcgc gattgcgatt gaccgcgacc
     1861 gcaacgtcgg catcgagatc ggcttcgacg gcgaggcgct gtgctcgcgc cgtctcgcct
     1921 tctgcaatgg cacgttccgc ctcacgcagc ttttcgcgcg cgagggcgag ctcggcgttg
     1981 ccgtactcgg ctgctcccgc gcgctcggcg tcggcgagac tgtcacgcgc gcggtccaag
     2041 tcgtcctcat atgaggctga cgccgccgtt tcgacgcggt tcgagctcgc gcatccgagc
     2101 atgacggcgc cgatcagcag caatccgaac agcatcacga ctcgttgatt tcgattcatg
     2161 tcggcatcct cgttggcttg ttcggcgcga tgcagattcc atgccactcg agtgccgcgc
     2221 gagtggcgca cacgtccagg tgaccatcgc gccgcgcgcg ctgcaccgat atcaattaca
     2281 gaatcgggtc gcaagggcgc caaccggcgc tcggccggga acagctggaa gcctggcacg
     2341 ggccttgcac tctgttcgca cccgattcaa tcgcgaaaag gagattcgcc gatggccgac
     2401 cctactgaaa atgaaggcgc cctgaaggac gccgccttga aagacgccaa aaatctcgcc
     2461 gctgcggcgc gcgaccgagg catggagcag ctcgagggcg caaagggcca gctggccgag
     2521 ggcgccgagc gagtcgcgtc agcggtcgag cgcacggcca acgagctcga tggcgacggc
     2581 gacggcgcga tctcgggcta cggccacagc ctcgcgaatt tgatgcgtca gctggccggc
     2641 ggattgcgtg agcgcgacat cgagcaattt gcgagcgagc tcgggtcctt ggcgcggcgc
     2701 aaccccggca tgttcttggc cggctccgtg gctctcggct tcggcgtcgc gcgcttcttc
     2761 aaagcgcgcg ctccgcagcg ccgcgccgac gacacgtacg gcggctacgc cggcggcagc
     2821 ggcggtaacg agtggcgcga tcgaagcgcg caggacggct atgaccgcgg cgagttcgac
     2881 gccgacgaga gtcttgatct ctcgggtaat ccaacgcgcg tccagcgccc cgatgacgac
     2941 gaggccaacg gcgcgatcgg cgacagcagt accgagcagt gggcgagaac cgagacgccg
     3001 gcaagcagca cgcaggattc gagcgccgcg cgcggcgacg agcggcaagc gaagtcacgt
     3061 tctggcaaga acaaggcgaa gacgcaacgc gcatcagccg gtgccaatcc gaaaccgacg
     3121 gaaggaccgt cgtcaccgct gggcgatagc tcggcgaccg acggctcgct gacgtcaggc
     3181 gacggagaca gcgctttcac gggcggtacg ggtggcggcg ggctgcgagg aggtaaggag
     3241 tcatgagcac tagaacgagc gatatgggtt cgcgtgagcg gaccgggccg cgcaacatcg
     3301 aggctcgcga ctacgagtcg acgccgacac tgctgcggcg gctcctcaac gacgtcggca
     3361 cgttgttcgc gcaagaggtg gcgttgctga aggccgagac gacggaatcg atcggcgacc
     3421 tcaaagccgc gacggtcagc atggcaatgg gcggcgccgt cgcattcatg ggcgcgttct
     3481 tcctgttgtt gtcggccgtc tacggcttga gcaacgtcgt cgatccatgg cttgcggcat
     3541 tgatcgtcgg cggcgtcgta acgctgatcg gcgtgatcat gctggtgaca ggcagcaaga
     3601 agctcgagcc tcgcgccgtc gcgccacgcc gcacggctgc ggctcttcgc agggacactg
     3661 agatggtcaa gggagttgcc caacatgagc acgagtgaga tggacatgaa acccacactg
     3721 gacgccgaca gcacgcggta cgccgagcac agcgattccg acgatcgtga cccggcggcg
     3781 ctcgagcgcg acatcgatac gacgcgcgcc gacgtgcgcg cgaccttggc cgcgctcgag
     3841 cggcgactgt cgttcgatcg gctcgtagag atgacggtag ggcggatccg cgagcgcggc
     3901 ggcgagttcg ccggcaactt gaccgacacg gcaacgcaga acccgatgcc ggtgctgctg
     3961 acgtcgatcg ggcttggctg gatgatgctc atgagccggc gcggcaatgg cgcctcgcag
     4021 ccgagccgcg cgcgagcgcg ggcagccgaa gcggccgaca gcgtttcggg cgcagccgaa
     4081 aggtttggcg agcggttctc gggcgcaaag gaccggttcg gcgagcaagt gtccggcgcg
     4141 gcccatggtg ccagcgagcg catgcacgaa gccggctctc gtttgcacga ggcagtcgag
     4201 tcgtcgcggc agacactcgg cgcaaccgcc gagtcgatgc gcgacaacgc gagccgtatg
     4261 gcgtccgcaa cgcgcgagca gatgagctat gccaccgacc gcatcgacca actgttgcac
     4321 gagcagccgt tgctgctggg cgcgcttggg ctcgcggcgg gcgcgttgat cggcgcgttg
     4381 ttgccgacgt cggaagccga aggtcgcttg ctcggcgaag cgcgcgacaa ggccgtcaag
     4441 aacgtggcgc aaaagagccg cacgctgtac gagacggcgc gcgacaacgc agcgcagttt
     4501 gcggctccgg gcggacgcga cgaagacgcg agcagcgagc gcccgtcacg cccgcactga
     4561 gctagtcggc cggcagatcc tcggcgtc
//
LOCUS       DATLEE010000608         3465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3359348, whole genome shotgun sequence.
ACCESSION   DATLEE010000608 DATLEE010000000
VERSION     DATLEE010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3359348"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VM692_13230"
     CDS             <1..336
                     /locus_tag="VM692_13230"
                     /inference="COORDINATES: protein motif:HMM:NF015867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HUQ53181.1"
                     /translation="LAPYITVLPRGTKLNVNTASDVVLASLSDDLDLGTAMGLVEERG
                     TAEFVDIDVTFEGLVDADTLQEIDGVSEHFLLTATVTLGTNQLTMRSVLQRDRSGITR
                     ALFRSLGVE"
     gene            338..1561
                     /gene="gspL"
                     /locus_tag="VM692_13235"
     CDS             338..1561
                     /gene="gspL"
                     /locus_tag="VM692_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HUQ53182.1"
                     /translation="MAETFFVRLSDSGTATWGAFDPTGGLVGGLGRGTLQSVQPALAG
                     RRCVALVNAVDVLTTDAALPAASQARLRQIVPFSLEESLADDLDQMVFAIGARLASGT
                     TQVAAVAKDRMDAWLGELRAAGIVPNAVYSEADGIPDIPSTLVLIIEGDRIAGRKPGQ
                     APFVFDGLDLRQVLALVRQRKPDEAELHHVRVFTDAAGRARFASELAALAGEFSSADV
                     KILNDGVFPHFAATLAQRGGTNLLQGPYAPKSNWLALVKPWRLAAYLVVASMLLALVL
                     QGAQLWQLERADAALTEAVASLCQRVVGESSTSACQREVAQRLGATAGSATEDFLSTL
                     AAIAAVRAPELRIEALSYRNRVMDLQLMAPSVTALDEFARAVEQTRRFDVEIEAQNPV
                     DTGTEGRLRIMGTNP"
     gene            1561..2037
                     /gene="gspM"
                     /locus_tag="VM692_13240"
     CDS             1561..2037
                     /gene="gspM"
                     /locus_tag="VM692_13240"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HUQ53183.1"
                     /translation="MARLKSWFFGLEERERWIVGVGAVVAAAIIIWGLGVRPMRAELA
                     RLRTAVDTKQRLLVEVARVEVAQPASVAGNRQGANQTINVILDTTARSYGIGQQRMRN
                     NGPSTVDVTLQGVPFDALVAWLVALHGTYGIDVETASFSSGRETGLVNGQVSLHRL"
     gene            2042..2797
                     /locus_tag="VM692_13245"
     CDS             2042..2797
                     /locus_tag="VM692_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HUQ53184.1"
                     /translation="MSRWSWWAVGFGAYVAFTLATFPAGTALHWFAPAELTAAGVTGT
                     LWSGSAASCSIAGFTAEALRWRVRPTSLLLGRIRAEVEARIPDGFVSGLVTASSSAVR
                     FNDLRGATSLPALASVLPLKGMRGQASVALSELVLDNGWPATIVGELKLAGLETVPLI
                     PDGSGSLLPLGDYTITFQPAPAGALAAMFVDNGGPLEVSGSFNMDDTRVYAIDALVEP
                     RADAPETLVEGLKIMTGDPDAEGRRRLSLTGSL"
     gene            complement(2775..>3465)
                     /locus_tag="VM692_13250"
     CDS             complement(2775..>3465)
                     /locus_tag="VM692_13250"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HUQ53185.1"
                     /translation="RGDAPTAPQRSEPTAIVPVLRRLVTTAAQPDAAPTAAVSATRPE
                     FVWAGQAAAHVGTVVHRHLQRIADQGLEDWDERRVTALQPMFARELELLGVEPAERAT
                     AAARVAAALTRALADPRGRWVLGPQAEARSELRLTLRAGSALEHVRLDRTFVADGRRW
                     IVDFKTSQHEGGSLSAFLASEVERHAPQLERYAQAVAVTDPRPIELGLYFPLLGELRS
                     WPSVATASRSG"
BASE COUNT          521 a         1150 c         1209 g          585 t
ORIGIN      
        1 ctcgctcctt atatcaccgt gttgccgcga ggcacgaaat tgaacgtcaa cacggcctcc
       61 gacgtggtgc tcgcatcgtt gtcggacgac ctcgacttgg gaacggccat gggcctcgtc
      121 gaggagcgcg gcacggcgga gttcgtcgac atcgatgtga cgttcgaagg gctcgtcgac
      181 gcggacacct tgcaagagat cgatggcgtc agcgagcatt ttttgttgac cgcgacggtg
      241 acgctgggta caaatcagct cacgatgcgc tccgtgctac agcgtgatcg cagtggcatc
      301 acgcgagcgt tgttccgcag tctcggagtc gagtagcttg gccgaaacgt tcttcgttcg
      361 tttgtccgac agcgggactg cgacctgggg tgcgttcgac cccacgggcg gcctcgtggg
      421 cggcctcggc cgtggcacct tgcaatccgt tcagccggcg ctcgccggtc gccgctgcgt
      481 agcactcgtc aatgccgtgg acgttctgac cacggacgcg gcgctgccgg cggcgagtca
      541 ggcgcgactg cggcagatcg tgccgttttc gctcgaggag tccttggcgg acgacctcga
      601 ccagatggtg ttcgcgatcg gagcgcgact cgcgtcgggc acgacgcagg tcgccgccgt
      661 cgcgaaggac cgcatggatg cgtggctcgg cgagctgcgc gccgcgggaa tagtgccaaa
      721 cgccgtctac tcggaagccg acggaattcc ggacatcccc tcgacgctcg tgctcatcat
      781 cgaaggcgac cgcatagcgg gacgcaaacc cgggcaagcg ccgtttgtat tcgacggcct
      841 cgacctgcgg caagtattgg cgctcgtgag gcagcgcaag cccgacgagg ccgagctgca
      901 ccacgtgcgc gtcttcaccg acgcggcggg ccgcgcacgg ttcgcttcgg agctcgccgc
      961 gcttgccggc gagttttcga gcgccgacgt gaagatcttg aacgacggcg tgttcccgca
     1021 cttcgccgcg actcttgcgc agcgcggcgg caccaatttg ctgcagggcc cctatgcgcc
     1081 gaaatcgaat tggcttgcgc tggtcaagcc ttggcgcttg gccgcgtatc tcgtcgtcgc
     1141 gagcatgctg ctggcgctcg tgttgcaggg tgcccagctt tggcagctcg agcgcgccga
     1201 cgccgcgctg accgaagccg tcgcgagctt gtgccagcgc gtcgtcggtg agtcgagcac
     1261 gtcggcgtgc cagcgtgagg tggcgcagcg gctcggcgcc actgcgggct cggcgaccga
     1321 ggactttctg agcacgcttg ccgcgatagc ggcggttcga gctccggagc tgcgcatcga
     1381 ggccttgagc tatcgcaatc gcgtcatgga tttgcagctc atggcgccga gcgtcacggc
     1441 gctcgacgag ttcgcacgcg ctgtcgagca aacgcgccgc ttcgacgtcg agatcgaggc
     1501 gcagaatccc gtcgacaccg gcaccgaagg gcgtctgcgg atcatgggga cgaacccatg
     1561 atggcgcggt tgaagagctg gtttttcggt ttggaggagc gcgagcgctg gatcgtcggc
     1621 gtcggcgccg tagtcgccgc cgcgatcatc atctgggggc tcggggtgcg ccccatgcgc
     1681 gccgagctcg cgaggttgcg cacggccgtc gacacgaagc agcgcttgct cgtcgaggtc
     1741 gcgcgcgtcg aagtcgcgca accggcgagc gtggccggca atcgtcaagg agccaaccag
     1801 acgatcaacg tcatcctcga taccaccgca cgctcgtacg gcatcggcca gcagcgcatg
     1861 cgcaacaacg ggccgagcac cgtcgacgtc acgctgcagg gcgtgccgtt cgacgcgctc
     1921 gtcgcctggc ttgtggcgtt gcacggcacg tacggcatcg acgtcgagac ggcgtcgttc
     1981 agcagcgggc gcgagaccgg cttggtgaac ggtcaagtgt cgctgcacag gctttgaagc
     2041 gatgagccgt tggtcatggt gggccgttgg tttcggcgca tacgtcgcgt tcacgttggc
     2101 gacatttccg gccggcaccg ccttgcactg gttcgcgcct gcagaactca cggcggccgg
     2161 cgtcacgggc acattgtggt ccggtagcgc cgcgagctgc tcgattgccg ggttcacggc
     2221 cgaagcgttg cgctggcgcg ttcgcccgac ttcgctgttg ctcgggcgga ttcgcgcgga
     2281 agtcgaggcg aggattcccg acgggttcgt gagcggcctc gttaccgcgt cttcttcggc
     2341 cgtgcgtttc aacgacctgc gcggcgccac gtcgttgccg gcgcttgcga gcgtgctgcc
     2401 gttgaaaggc atgcgcggcc aagccagcgt ggcgctttcg gagctcgtgc tcgacaacgg
     2461 ttggcccgcg acgatcgtcg gcgagctgaa gctggccggg ctcgaaacgg taccgctgat
     2521 tcccgacggc agcggctcgt tgctgccgct gggcgactac acgatcacgt tccaaccggc
     2581 tcctgccgga gcgctcgccg cgatgttcgt cgacaacggc gggccgctcg aggtctcggg
     2641 tagcttcaac atggacgaca cgcgcgtcta tgccatcgat gcgctggtcg agccgcgtgc
     2701 cgacgcgccg gaaacgctcg tcgaagggct gaaaatcatg acaggcgatc ccgacgccga
     2761 aggccgccgg cgcctcagcc tgaccggctc gctgtagcga cggacggcca cgagcgaagc
     2821 tcgccgagca acgggaaata caagccgagc tcgatcggtc gcggatccgt gactgcgaca
     2881 gcctgcgcgt agcgctcgag ctgcggcgca tgccgctcga cttccgatgc gaggaaggcg
     2941 ctcaagctgc cgccttcgtg ctggctcgtc ttgaagtcga cgatccagcg gcggccgtcc
     3001 gcgacgaaag tccgatcgag gcgcacgtgc tcgagcgcgc tgccggcgcg cagcgtcagc
     3061 ctgagctccg agcgcgcctc cgcctgcggg ccgagcaccc atcgcccgcg cggatccgcg
     3121 agcgcgcgcg tcagcgcggc cgccacacgc gcggccgcgg tggcgcgttc cgcgggctca
     3181 accccgagca actcgagctc gcgggcgaac atcggctgca gcgcggtcac acgccgctcg
     3241 tcccaatcct cgagcccctg atctgcgatg cgttgcaagt gacggtgcac gacggtgccg
     3301 acgtgcgccg cggcttgccc ggcccagacg aactcgggcc gcgtcgcgga aacggcggct
     3361 gtcggcgccg cgtcgggctg cgccgccgtc gtcacgaggc gacgcagcac cggcacgatc
     3421 gccgtcggct cgctgcgttg cggcgccgtc ggcgcgtcgc cgcgc
//
LOCUS       DATLEE010000609         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7810047, whole genome shotgun sequence.
ACCESSION   DATLEE010000609 DATLEE010000000
VERSION     DATLEE010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7810047"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VM692_13255"
     CDS             <1..274
                     /locus_tag="VM692_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53186.1"
                     /translation="NLSMVTARRVPIVDEEAQVVLGLGVFIRRPGSSTPRNVFSEWFV
                     IDNARIRTIYTSMFYPPPELAVPNWPPYDGNWPLPATTVPELTPRP"
     gene            complement(281..1270)
                     /locus_tag="VM692_13260"
     CDS             complement(281..1270)
                     /locus_tag="VM692_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006069430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HUQ53187.1"
                     /translation="MDPFSHALLGASCGQARARREQLVAAGGVALVAAIAPDLDALIQ
                     SSSDPLLVLEYHRQFTHSLPFAPIGALLCAAACWWFVRSRLSFRHAYVLALLGYTSHL
                     LLDACTTYGTQLLWPFADTRFAWNFIAVIDPLFTLPVLVLVIAALLARRVGLARAAIA
                     WAVAYVALGALQHSRATAAAMELAASRGHQPETLVVTPALFSLVLWKTVYEHAGTFHV
                     DAVRAGFVTSTLPGETIAKLDAARDFPWLAPTSQQAIDVERFARVAHGLVAVDETAAD
                     RVIDLRYSLVPNEIAGFWAIVLNPEAGPSEHVDYVVTREQAPEQARRLLAMLF"
     gene            1333..>2062
                     /locus_tag="VM692_13265"
     CDS             1333..>2062
                     /locus_tag="VM692_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53188.1"
                     /translation="MRRNTALALLTVMLSPAAAQTPPAAAAAPLVFPEGLPEWAFNIP
                     DAVQPPTPPIAGIVHGPGSAREYDAAAIAGNNNPPDWFPEEHPPAPRVVAGPAPNACG
                     SCHLMSGRGHPESADVAGLPVAYFIRQMHDFKTGARREVNRMEPIARALSDEDVRLAA
                     EYFAALAPTPFVDVIETATPPKTYVSANARHRVLSPDGATEPMGRRIVQIPKDPFRTN
                     LRDPKSGFIAYVPPGSLRRGEELVT"
BASE COUNT          361 a          716 c          680 g          305 t
ORIGIN      
        1 caatctctcg atggtgacgg ctcgccgcgt tccgatcgtc gacgaggagg cgcaggtcgt
       61 gctcggcctc ggagtgttca tccgccggcc cggctcgtcc acgccgcgca atgtcttcag
      121 cgaatggttc gtgatcgaca atgcgaggat ccgcacgatt tacacgtcga tgttttaccc
      181 gccgcccgag ctcgccgtgc cgaactggcc gccgtacgac ggcaactggc cgttgccggc
      241 gacgaccgtg ccggagctca cgccgcgccc gtagcgctgc tcaaaagagc atcgcgagga
      301 ggcgccgcgc ctgctccggt gcctgctcgc gcgttacgac atagtcgacg tgctccgaag
      361 gtcccgcctc cggattgagg acgatcgccc aaaatcctgc gatctcgttc ggcacgagcg
      421 agtagcgcag gtcgatgacg cggtccgccg ctgtctcgtc gacggcgacg aggccgtgcg
      481 ccacgcgtgc gaaacgctcg acgtcgatcg cttgctggct cgtgggcgcc agccacggga
      541 agtcgcgtgc agcatcgagc ttggcgatcg tctcaccggg cagcgtgctc gtcacgaagc
      601 cggcgcgcac ggcatccacg tggaaagtgc cggcgtgctc gtagacggtc ttccagagca
      661 ccaggctgaa tagcgccggc gtcacgacga gcgtctcggg ctgatgccct cggctcgccg
      721 caagctccat tgcggccgcg gtcgcgcgcg agtgctgcaa ggcgcccagt gcgacgtacg
      781 caacggccca cgcgattgcc gcgcgcgcga ggccgacgcg tcgtgcaagc agcgcggcga
      841 tcacgagcac gagcacgggc agcgtgaaca agggatcgat gaccgcgatg aagttccaag
      901 cgaagcgcgt gtccgcgaac ggccaaagta gttgagtgcc atacgtcgtg cacgcgtcga
      961 gcaacaagtg gctggtgtag ccaagcaacg ccagcacgta cgcgtgccga aacgataggc
     1021 gcgagcggac gaaccaccag cacgccgccg cgcacagcag cgctccgatc ggcgcgaacg
     1081 ggagcgagtg cgtgaattgc cggtggtact cgagcaccag cagagggtcc gacgacgatt
     1141 ggatcaacgc gtcgagatcc ggggcgatcg cggccacgag cgcgaccccg cccgccgcga
     1201 cgagttgctc gcggcgtgcg cgcgcctgac cgcagcttgc accgagcaac gcatgactga
     1261 acggatccat gccgcattct atccgccacg tgcgacagcg gtgccggcat gccttcggat
     1321 agactgctcg acatgaggcg caacaccgca ttggcgctgc tgaccgtcat gttgtcgccg
     1381 gcggccgcac agacgccgcc cgcggcggca gcagcgccgt tggtcttccc cgaaggcctg
     1441 cccgaatggg cgttcaacat tcccgacgcc gtccaaccgc cgacgccgcc gatcgcgggg
     1501 atcgtgcatg gaccgggcag cgcgcgcgaa tacgacgcgg ccgcgattgc cggcaacaac
     1561 aatccacccg actggtttcc cgaggagcat ccgccggcgc cgcgagtcgt cgcgggtccg
     1621 gcgccgaatg cctgcggctc gtgccatctg atgtcggggc ggggccaccc ggagtctgct
     1681 gacgtcgcgg gcctaccggt cgcgtatttc attcggcaga tgcacgactt caagaccggc
     1741 gcgcgccgcg aggtcaatcg catggagccc atcgcacggg cgctgtcgga cgaggacgtc
     1801 cgtctcgccg ccgagtactt cgcagcactg gcgccgacgc cgttcgtcga tgtcatcgaa
     1861 acagccacgc cgccgaaaac ctacgtcagc gctaatgccc ggcaccgcgt gctatcgccc
     1921 gacggcgcca ccgagccgat gggccgccgc atcgtgcaga taccgaagga tccgtttcgc
     1981 accaacctcc gggatccgaa atccggtttc atcgcctatg tcccgcccgg cagcctccgc
     2041 cgcggcgagg agctcgtaac aa
//
LOCUS       DATLEE010000610         3079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_735827, whole genome shotgun sequence.
ACCESSION   DATLEE010000610 DATLEE010000000
VERSION     DATLEE010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735827"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1207
                     /locus_tag="VM692_13270"
     CDS             335..1207
                     /locus_tag="VM692_13270"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HUQ53189.1"
                     /translation="MSSASAVKSVPIATLITDACAKLLSNQTSLPSMPAVAARIHDAM
                     ASSNWSMRTIAAIIKSDVGTTTYLLHVANSPLYAGATPVRQVEQAIARLGINSTRHLV
                     MAHAVRSTFVTRSPLLTSLMQRTWASSTRLAALCGVLAKHCSSFRPESATLAGLLQDI
                     GVLPILNVLNRYQDQLSSEAPVLSAIDKFAPPVGTILLSRWGFEADMVEVARSRGNWL
                     RHPQPAADLADLVLVARLHANITSGNREHSPRIDAVPAFAKLPLGSLEPDGSIALLHD
                     EEATIDELMGSLAA"
     gene            complement(1209..2489)
                     /locus_tag="VM692_13275"
     CDS             complement(1209..2489)
                     /locus_tag="VM692_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53190.1"
                     /translation="MPHPAIARARQATISSPSGNGQLSLGNADGDTIVQAGMTVDGLG
                     VARAGPAMCGPPGWAPTPRVAVRDSRQEGALTRAMLGSVELRGRRTMMSIYQEKSMNR
                     TKCAGLVVVGLAATISQALAAPPAEIVIPGERLATESLTSTRDGTVYIGSVGTGQIFR
                     ARPGAANAEPFIAAKTNGIGAVFGVFADEKAGTLWACSGSFPPGPPPAGAPAPPPSAL
                     YAFDLKSGAAKGKWNMPTAGAFCNDIAVGGDGTAYASDTQNMQVARLKPGAQSLDVWA
                     GADGAFGPSGGVLDGIAVLGNTLLVNALRTNKIFSVAIGSDGKAGKPAEVKLDSAVEG
                     PDGMRSFGANALLVAEGGGSGSVTKIVLNDAKTEGRRTVLKSGFPDGPVAVTVVEESV
                     YVLEGQLAALFAPPGSPPPPVKPYKATAVPVGKP"
     gene            2488..>3079
                     /locus_tag="VM692_13280"
     CDS             2488..>3079
                     /locus_tag="VM692_13280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUQ53191.1"
                     /translation="MRTLLCAIAALAGHALVAQERSTANQSPAEIVATAPAADWVRIA
                     PSGLLVMELAPSRDGTPRRVTIQLIAPPFSEPWVDNIRKLAASHWWDGLSVNRVQDNY
                     VVQWGDPDAEDPTKARALPPALAMPDENAYLAPRRQLTSAALGGAALARDSYATSTGF
                     TATGWPYASDDDAAWPVHCYGMVGVGRGLSPDTGTGA"
BASE COUNT          491 a         1075 c         1004 g          509 t
ORIGIN      
        1 ggcgctttca tccacggcgg aacgtctaac ggctccgcgc cgacgggcct agcggacaac
       61 agggtgaggc cgccggcgag cgcagcgcct tgtgcgagaa acagccgccg gtgcagcaga
      121 ccattgcccg cgacgggttc cagcccggtc gattcgtgtt tcttcatgag caatctccca
      181 acggcgtcgt cgctgccaat gtaggcggcg caagcacgcc tgtaaacgag tggcatgagt
      241 cccactaatc ggaaccgttg cacgccacag tctcaacctt tccgccgagc ggccgactaa
      301 gcgagcagct cgcaaaccat ggagatcgtt gaccgtgagc tcagcgtcgg cagtgaagtc
      361 cgttccgatt gcgacactca tcacggacgc ttgcgccaag ctgctgagca atcagaccag
      421 cctgcccagc atgccggcgg tcgccgcgcg catccacgac gcgatggcga gctccaactg
      481 gagcatgcgc acgatcgcgg cgatcatcaa aagcgacgtc ggcacgacga cctatctgct
      541 gcacgtcgcc aacagcccgc tctatgcagg agcgacgccg gtgcgccagg tcgagcaagc
      601 gatcgcgcgt ctcggcatca acagcacgcg ccatctcgtg atggcccacg ccgtgcgctc
      661 gacgttcgtg acccgctcgc cgttgctgac gtcgctgatg caacgcactt gggcgtccag
      721 cacgcggctt gccgcgctgt gcggcgtgct cgcgaaacat tgctcgagtt ttcgcccgga
      781 gagcgcgacg ctggccgggt tgctgcagga catcggcgtg ctgccgattc ttaacgtgct
      841 caaccgctac caggaccagc tgtcgagcga agcccccgtc ttgagtgcaa tcgacaaatt
      901 cgcgcctccg gtcggcacga tcttgctctc acggtggggg ttcgaggccg atatggtcga
      961 ggttgcgcgg agccgcggaa actggctgcg gcatccgcaa cctgctgcgg atctcgcgga
     1021 cttggtgctg gtcgcgcgcc tgcacgcaaa catcacctcg ggcaaccgcg agcactcgcc
     1081 gcgcatcgac gccgtgccgg cgtttgcgaa gttgccgctc ggcagtctcg agccggacgg
     1141 cagcatcgcc ttgctgcacg acgaagaagc cacgatcgac gagctgatgg gctcgctcgc
     1201 cgcttgattc agggcttgcc gacgggcacg gctgtcgcct tatacggctt cacgggcgga
     1261 ggcggcgagc ccggcggcgc gaacaacgcc gcgagttggc cctcgagcac gtacacgctc
     1321 tcctccacga cggtcacggc gacggggccg tcgggaaagc cggacttgag cacggtccgt
     1381 ctgccttcgg tcttcgcgtc gttcagcacg atcttggtca cgctgccgct tcccccgcct
     1441 tccgcgacga gcaacgcatt cgcgccgaac gagcgcatgc cgtcggggcc ttcgacggcg
     1501 gagtcgagct tcacctcagc aggcttgccg gccttgccgt cgctgccgat ggcgacgctg
     1561 aagatcttgt tcgttcgcag cgcgttgacg agcagcgtgt tgccgagcac ggcgataccg
     1621 tcgagcacgc cgccgctcgg accgaacgcg ccgtcggcgc cggcccacac gtcgagcgac
     1681 tgtgcgccgg gcttgagtcg cgcaacctgc atgttctgtg tgtcgctcgc atacgcggtg
     1741 ccgtcgccgc ccactgcaat gtcgttacag aacgcgcccg ccgtcggcat gttccacttg
     1801 cctttcgcgg caccgctctt gagatcgaac gcgtagagcg cggacggcgg cggcgccgga
     1861 gccccggccg gcggcggtcc gggcgggaat gagcccgagc aggcccacag cgtgccggcc
     1921 ttctcgtcgg cgaacacgcc gaagaccgcg ccgatgccgt tcgtcttcgc cgcgatgaac
     1981 ggctcggcgt tagcggcgcc tggccgcgcg cggaagatct ggccggtgcc gacgctgccg
     2041 atgtaaacgg tgccgtccct tgtcgaggtg aggctttcgg tcgcgaggcg ctcgcctgga
     2101 atgacgatct ccgcaggtgg ggctgcgagc gcctgcgaga tcgtcgcggc gagcccgact
     2161 acgacgagac cggcgcactt cgttctgttc attgatttct cctggtagat cgacatcatg
     2221 gtgcgcctgc cgcgcagctc gacgctacct agcatagccc gggtcagcgc tccttcttgc
     2281 cgagaatcgc gaacggcaac ccttggagtt ggagcccagc ccggcgggcc gcacatcgct
     2341 gggcccgcgc gcgcgacgcc gagcccgtcg acggtcatac cggcctgcac gatcgtgtcg
     2401 ccgtccgcgt tgccgagcga caactggccg ttgccgctcg gcgacgagat cgttgcttgc
     2461 cgcgcgcgcg caatggcagg atgcggcatg cgcaccctcc tctgcgccat cgccgcgctt
     2521 gccggccacg cgcttgtcgc gcaggagcgc agcaccgcta accaatcacc ggccgaaatc
     2581 gtcgcgacgg cccccgccgc cgattgggta cgaatcgcgc cgtccggtct gctcgtgatg
     2641 gagcttgcgc cgagccgcga cggcacgccg cgccgcgtaa ccattcagct catcgcaccg
     2701 ccgttctccg agccgtgggt cgacaatatt cgcaagctcg ccgcgtcaca ctggtgggat
     2761 gggctcagcg tgaaccgcgt gcaggacaac tatgtcgtgc agtggggcga tcccgatgcg
     2821 gaagatccca cgaaggcacg cgctctgccg cccgcgctcg ccatgccgga cgagaacgcc
     2881 tacctcgctc cgagacggca actgacatcg gctgcgctcg gcggagccgc gctcgcgcgc
     2941 gacagctatg cgacctctac gggattcacg gcgaccggtt ggccgtatgc gagcgacgat
     3001 gacgccgctt ggcctgtcca ttgctacggc atggtcggcg tcggccgcgg cctctcgccc
     3061 gacaccggca ccggcgcag
//
LOCUS       DATLEE010000611         6658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8564518, whole genome shotgun sequence.
ACCESSION   DATLEE010000611 DATLEE010000000
VERSION     DATLEE010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8564518"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VM692_13285"
     CDS             complement(<1..834)
                     /locus_tag="VM692_13285"
                     /inference="COORDINATES: protein motif:HMM:NF017709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUQ53192.1"
                     /translation="MPIAPRAVAWLAAAFLTPAAAQVTHVQPTQPVQLLGPLQPVKGG
                     SAVYIVKLRTPGAASYKRSPTDFSAAKPSELARSARSAAAEAYAKQVEQTHDRLLGSI
                     GAASSKLYSYRYSVNGFAAKLTAAQVARLAQSPEVERIWQDTDRYLQTNNSANFLGLQ
                     DPMGGLRADLGLRGENIVVGIIDSGIAPQHPSLRDTEDRTPRACRSDWSRTSWLGIWL
                     CTSYRRNPPTVVVYDPPVGFTGTCQAGDGFAATDCNNKVVGARFYLDGFLARHELDRG
                     EF"
     gene            complement(914..1468)
                     /locus_tag="VM692_13290"
     CDS             complement(914..1468)
                     /locus_tag="VM692_13290"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HUQ53193.1"
                     /translation="MGLLALRHAARIVRSGGVIAYPTEAVFGLGCLPRDRAAVARVLA
                     IKRRSWRKGLILIGADVAQLEPYVELPPAPLRSQVLGSWPGPHTWVLDARPDAPRLIT
                     GGRSSVAVRVTAHPLARDLCLAVGEALVSTSANVSRRPPHRRLLRLRRDLGGQVDYVL
                     AGALGGLAEPTVIRDGRTGGVLRG"
     gene            complement(1468..3945)
                     /locus_tag="VM692_13295"
     CDS             complement(1468..3945)
                     /locus_tag="VM692_13295"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HUQ53194.1"
                     /translation="MGKNLVIVESPAKARTIEKYLGSDFEVLASYGHVRDLVPKEGAV
                     DPENGFAMKYQLIDKNEKHVDKIVKAAKKASAVYLATDPDREGEAISWHVYEVLKERK
                     VLDSKSVQRVVFHEVTKRAIQEAIDQPRDLSEQLVNAQQARRALDYLVGFNLSPLLWK
                     KVRRGLSAGRVQSPALRLIVEREDEITAFKAQEYWTIEAVAEQASQAFPARLAVFDRE
                     KVEQFTFTGEAQVRSAEQKIRAKAAGVLTVAKVDKKQRRRNPAAPFTTSTLQQEASRK
                     LGFSAQRTMTVAQRLYEGIDIGEGSVGLISYMRTDSVTLADEALVEIRDVIKERFGAE
                     NLPEEPRRYTTKAKNAQEAHEAVRPTSAARHPEDLRRFLDPNQYKLYELIWKRTVASQ
                     MNPAVYDTVALDLAAGESPDGEISFRATGSVLVSPGFIAVYQEGQDDTLADEQDRILP
                     AVAEGDRLSLKDVQSAQHFTEPPPRYSEATLVKALEEFGIGRPSTYASIISTLRNREY
                     VEMENRRFIATDVGRVVNRFLTRFFSQYVDYEFTARMEDSLDAVSRGEREWVPLLDEF
                     WKPFTKLVEHTDMNVTREQASQSRVLGTDPLSGRPVSVRMGRYGAFVQIGTKEDVEKP
                     KFAGLRPGQKMDTIELPAALDLFKLPRELGATPEGEPVSANIGRFGPYVRYANKFVSI
                     KGDDPYTITLERALELVAEKKITDANRLILDFPDAGIQVLNGRYGPYITNKLKNAKIP
                     KDKTPKELTLDECKALLAAAPERRGRGMKRKPAPAAAAEAPAETAAPAEKAAAPKKKA
                     KKKKKKAATRKQAAAAKAVDAGEPPAE"
     gene            complement(4111..4578)
                     /locus_tag="VM692_13300"
     CDS             complement(4111..4578)
                     /locus_tag="VM692_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HUQ53195.1"
                     /translation="MKESVLDVLMYLFESYVESQDEPEPNRHDLKRELGRAGFHDGEI
                     ERALDWLDGLNCSGATSAPQGTAFRVFDAAEQERLDATSRGYLLQLEQIGILQPAQRE
                     IAIDRLLALDNEEIDVEQTKWVVMMVLFSQPGQQEAYAQMEDLIYADDSAWVH"
     gene            complement(4578..5699)
                     /gene="dprA"
                     /locus_tag="VM692_13305"
     CDS             complement(4578..5699)
                     /gene="dprA"
                     /locus_tag="VM692_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HUQ53196.1"
                     /translation="MGLGAWLTLLAAAQQVSPRPWLDALKRCGSIEALCRSPRLLREC
                     GVAEDAIAKLDTPDAKLIERCREWLARPRRTLITFGSDGYPSRLAQLPDAPLALWVEG
                     SELSLLKSPQLAMVGSRSPTTGGRETAEQFARYLSERGLTITSGLATGIDGASHRGAL
                     PALGSTVAVLGCGIDVVFPRAHAQLAAEIAAKGLIVSEYAPGVEPQKFFFPARNRIIA
                     GLSLGTLVVEATRRSGSLITAKRAMEYGREVFAIPGSIHNPLARGCHLLIREGAKLVE
                     EAADILIELAPQLTAEGIELKSVESTPMGPDSTSLIDEPSYQNLLKKMEFSPVAITEL
                     GARAHLTTAEVSSMLLVLELEGLVEALPGGWYSRLGKKK"
     gene            complement(5787..>6658)
                     /locus_tag="VM692_13310"
     CDS             complement(5787..>6658)
                     /locus_tag="VM692_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HUQ53197.1"
                     /translation="QINPQVENPHLIFPGDILSLAYLGDGRPVVQLERGPQNVQAGSG
                     VTRLSPRVRSEPLEEAINTIPYDTIAAFLSRPRVIEEDEVDELPYIMAHREGLIGSAG
                     RDVYVRGTDAPTGSVFNVVERGAELVDPDTKDVLGYQGIYVGQGRLDRTGDPSTVRML
                     ETEREAVVGNLLMSEEDVQPMNFLPRSPDREVEGRIISVMSGVSLIGQYAVVVINRGS
                     EAGLEPGHVLRVYQTGRTIRDTQRGAFGKKVRLPDEPAGTMMVFRTSERLSYALIMEA
                     TTPLALLDTVRNP"
BASE COUNT         1022 a         2182 c         2240 g         1214 t
ORIGIN      
        1 gaactcgccg cgatcgagct cgtggcgcgc caagaacccg tcgagataaa agcgcgcgcc
       61 gacgaccttg ttgttgcagt ccgtcgccgc gaatccgtcg ccggcttggc acgtgcccgt
      121 gaacccgacc ggcgggtcgt agacgaccac ggttggcgga ttgcggcggt acgacgtaca
      181 gagccagatg ccgagccacg acgtgcgcga ccagtcgctg cggcacgcgc gcggcgtgcg
      241 gtcttcggtg tcgcgcagcg acgggtgctg cggggcgatg ccgctgtcga tgatgccgac
      301 gacgatgttt tcgccgcgca acccgagatc cgcgcgcagc ccgcccatcg ggtcctgcag
      361 accgaggaag ttcgcgctgt tgttcgtctg cagatagcgg tcggtgtctt gccagatccg
      421 ctcgacctcg gggctttgcg ccaggcgcgc gacttgggcc gcggtcagtt tcgccgcaaa
      481 accgttgacc gagtagcgat agctatagag cttcgagctc gcggcgccga tgctgccgag
      541 caaccggtcg tgcgtctgct ctacctgctt cgcgtaggcc tcggctgccg cgctgcgagc
      601 gctcctcgcg agctcgctgg gcttcgcggc cgagaagtcc gtcggcgagc gcttgtagct
      661 cgcggctccc ggtgtccgca gcttgacgat gtagacggcc gagccgccct tgacgggctg
      721 cagcggtccc aagagctgca ccggctgagt gggctgcacg tgcgttactt gcgcggccgc
      781 gggcgtcaga aatgcggccg cgagccacgc tacggctcgg ggggcgatgg gcattttcgt
      841 tctaattcgg gccttgcagc agagtgccgc agacgttagc acagccgaac ggcgcgaacg
      901 ttacggccgg cgctcagccg cgcagcacgc cgcccgtgcg accgtcgcga atgaccgtgg
      961 gctcggcgag gccgccgagc gcgcccgcga gcacgtagtc gacttgaccc ccgaggtcgc
     1021 ggcgcagccg cagcaggcgg cgatgcggcg gccggcggct gacgttggcg ctcgtggaaa
     1081 ccagagcctc gccgacggcg aggcacaggt cgcgcgccag cgggtgcgcg gtcactcgca
     1141 ccgcgacgct cgagcgcccg cccgtgatca agcgtggggc gtcgggccgc gcgtcgagca
     1201 cccaggtatg cggaccaggc cagctgccga gcacttggct gcgcagcggc gccggcggca
     1261 attccacgta gggttcgagc tgcgcgacat cggcgccgat gaggatcaag cctttgcgcc
     1321 atgagcgtcg cttgatggcc agcacgcgcg cgacggcggc gcggtcacgc ggcaggcaac
     1381 ccaagccgaa tacggcttcg gtcgggtaag cgatcacgcc gccgctgcgc acgatgcggg
     1441 cggcgtgacg cagcgcgagg agccccatca ctccgccggc ggctcgccgg cgtctacggc
     1501 tttggcagcg gcggcttgtt tgcgcgtcgc ggccttcttc tttttcttct tggctttttt
     1561 cttcggcgcg gctgcttttt cggcgggcgc ggcggtctct gccggggcct cggccgcagc
     1621 ggccggcgcg ggtttgcgtt tcatgccgcg gccgcgccgc tcgggcgccg ccgccagcaa
     1681 cgccttgcat tcgtcgagcg tcagctcctt cggcgtcttg tccttcggga tcttggcgtt
     1741 cttgagcttg ttcgtgatgt acgggccgta gcggccgttc aacacctgga tgccggcgtc
     1801 cgggaaatcg aggatcagac gattggcgtc cgtgatcttc ttctcggcga cgagctcgag
     1861 cgcgcgctcg agcgtgatcg tgtacggatc gtcgcccttg atcgacacga atttgttcgc
     1921 gtagcgcacg tacggcccga accggccgat gttcgccgag acgggctcgc cttcgggcgt
     1981 cgcgccgagc tcgcgcggca acttgaacaa gtcgagcgcc gcgggcagct cgatcgtgtc
     2041 catcttttgg ccggggcgca ggcccgcgaa cttcggcttc tcgacgtcct ctttcgtgcc
     2101 gatctgcacg aaagcgccgt agcggcccat gcgcacgctg acaggacggc cgctgagggg
     2161 gtcggtgccg agcactcgcg attgcgacgc ctgctcgcgg gtcacgttca tgtcggtgtg
     2221 ctcgacgagc ttcgtgaacg gtttccagaa ctcgtcgagc agcggcaccc actcgcgctc
     2281 gccgcgcgac accgcgtcga gcgagtcctc catgcgcgcc gtgaactcgt agtcgacgta
     2341 ctgcgagaag aaccgcgtca ggaagcgatt gacgacgcgg ccgacgtcgg tcgcaatgaa
     2401 gcgccgattc tccatttcga cgtactcgcg gttgcgcagc gtcgagatga tcgacgcgta
     2461 cgtcgatggc cggccgatgc cgaactcttc gagcgctttg acgagcgtcg cttccgagta
     2521 ccgcggcggc ggctccgtga agtgctgcgc gctctgcacg tccttcaggc tcaagcggtc
     2581 gccttcggcc acagccggaa ggatgcggtc ctgctcgtcg gcgagggtgt cgtcctggcc
     2641 ttcctggtac acggcgatga agccggggct cacgagcacg gagcccgtcg cgcggaacga
     2701 gatctcgccg tccggcgact cgccggccgc gaggtcgagc gcgaccgtgt cgtagacggc
     2761 cgggttcatc tgcgacgcaa cggtgcgttt ccagatgagc tcgtagagct tgtactgatt
     2821 cggatcgagg aatcggcgca ggtcctcggg atgacgcgcg gccgacgtcg gccggacagc
     2881 ttcgtgcgct tcctgcgcgt tcttggcctt cgtcgtgtag cgccgcggct cctcgggcag
     2941 gttctcagcg ccgaagcgtt ccttgatgac gtcgcggatc tcgacgagcg cttcgtcggc
     3001 cagcgtcacg gagtccgtgc gcatgtagct gatgaggccg accgagccct cgccgatgtc
     3061 gataccttca tataggcgct gcgcgacggt catcgtgcgc tgcgcgctga agccgagctt
     3121 gcgcgatgct tcctgctgta gcgtcgacgt cgtgaacggt gcggcgggat ttcggcgccg
     3181 ctgcttcttg tcgaccttgg cgacggtcag gacgccggcc gccttggcgc gaatcttctg
     3241 ctcggcgctg cgcacctgcg cttcgcccgt gaacgtgaat tgctcgacct tctcgcggtc
     3301 gaagacggcc agccgcgccg ggaacgcttg gctcgcttgc tcggcgacgg cctcgatcgt
     3361 ccagtattcc tgcgctttga acgccgtgat ctcgtcctcg cgctcgacga tcaagcgcaa
     3421 cgcggggctc tgcacgcggc ccgcggacaa cccgcgccgc actttcttcc agagcagcgg
     3481 cgacaggttg aagccgacga gatagtcgag cgcacgtcgc gcctgctgcg cgttgacgag
     3541 ctgctcggac agatcgcgcg gctgatcgat ggcttcctga atggcccgtt tagtgacctc
     3601 gtgaaacacg acccgctgca ccgacttcga gtcgagcacc ttgcgttctt tcagcacctc
     3661 gtagacgtgc cacgagatgg cttcgccctc gcggtccggg tccgtcgcga ggtagacggc
     3721 cgacgccttc ttcgcggcct tgacgatctt gtcgacgtgc ttctcgttct tgtcgatgag
     3781 ctgatacttc atcgcgaagc cgttctcggg atcgactgcg ccttccttcg gcaccaagtc
     3841 gcgcacgtgg ccgtaggagg cgagcacctc gaagtcgctg ccgagatatt tctcgatagt
     3901 gcgcgccttg gccggcgact cgacgatgac gaggttcttg cccatgactc agcggcggct
     3961 gcggtcagct cgaggtgatc tcagttgcgg aggcatgaaa tgcagacagg cctcatctcg
     4021 tggggtgggc gtttgcaaag gaccggcagg atagccgatt cgcgcggcgg cgttaaagac
     4081 ggcttcgtga gactgagcgc gggcgctgtg ctagtggacc caggccgagt cgtccgcgta
     4141 aatgaggtcc tccatctgcg cgtatgcctc ttgctggccg ggctgactga acaacaccat
     4201 catgaccacc cacttggtct gctcgacgtc gatctcctcg ttgtcgagcg ccaacagccg
     4261 atcgatggcg atctcgcgtt gggcgggttg caaaattccg atttgctcga gctgcagcag
     4321 atagccgcgc gacgtggcgt cgagccgctc ttgctcggcc gcatcgaaaa cgcggaacgc
     4381 cgtcccttgg ggcgcgctgg tcgcacccga gcaattcagg ccatcgagcc agtcgagcgc
     4441 gcgctcgatt tcgccatcgt gaaaaccggc gcgaccgagc tctcgcttga ggtcgtgtct
     4501 gttcggctcc ggctcatctt gggattcgac gtaggactcg aacaggtaca tcagtacgtc
     4561 gaggacggac tctttcatca tttcttcttg cccagacgcg aataccaacc gccgggcaac
     4621 gcctcgacca acccttcgag ctccagcacc aggagcatgg aggaaacctc cgccgtcgtc
     4681 aaatgcgcgc gggctccaag ctcggtaatg gcaacgggcg aaaactccat ttttttcaac
     4741 aagttttggt agctcggctc gtcgatgagc gacgtcgaat cgggccccat tggggtgctc
     4801 tcgacgctct tgagctcgat gccttcggcc gtcagttggg gcgcgagctc gatgagaata
     4861 tcggcggcct cttcgacgag cttggcgcct tcacggatca agagatggca gccgcgcgcg
     4921 agcggattgt ggatcgaccc cgggatcgcg aaaacctcgc gcccatactc catcgcgcgc
     4981 ttggccgtga tcagcgagcc gctgcgccgc gtcgcctcga cgacgagcgt cccgagcgac
     5041 agacccgcga tgatgcgatt gcgcgccgga aagaaaaatt tctgcggctc gacgccgggc
     5101 gcgtactccg aaacgatcag gcccttcgcc gcgatctccg ccgcgagctg cgcgtgcgcg
     5161 cgcgggaaca cgacgtcgat cccgcagccg agcactgcca ccgtgctgcc gagcgccggc
     5221 agggcgccgc ggtggctcgc gccgtcgatg ccggtcgcga ggccgctcgt gatcgtgagg
     5281 ccccgctcgc tgagatagcg cgcgaattgc tcggctgtct cgcgcccgcc cgttgtcggg
     5341 ctgcggctgc cgaccatcgc gagttgtggg gacttgagta acgagagctc ggagccctcg
     5401 acccatagcg ccagcggcgc gtcgggcagc tgcgcgaggc gggacggata gccgtcgcta
     5461 ccgaatgtaa ttagcgtacg gcgcggccgc gcgagccact cgcggcagcg ctcgatgagc
     5521 ttggcgtcgg gtgtgtcgag cttggcgatg gcgtcttcgg ccacgccgca ctcgcgcagc
     5581 agtcgcggcg agcggcacag ggcttcgatc gagccgcagc gcttcagcgc gtcgagccaa
     5641 ggtcgcgggg agacttgctg cgctgccgcc agcagcgtca gccaggcccc taggcccaag
     5701 gccgcgtccc ccgtcgggga cttccaaggg tcgtccccgt tcggggacgt ccaagagttg
     5761 tcgcctgcgg gcgagagaaa ttcgggctac gggttacgaa ccgtatcgag cagtgccaac
     5821 ggcgtcgtgg cttccatgat gagggcatag ctcaagcgtt ccgacgtgcg gaacaccatc
     5881 atcgtgccgg ccggctcgtc cggcaatcgc actttcttgc cgaaagcacc acgttgcgtg
     5941 tcgcggatcg tgcggcccgt ctgatacacg cgcagcacgt gaccgggctc gagcccagcc
     6001 tccgagccgc ggttgatcac gacgactgcg tattggccga tcagcgaaac gccagacatc
     6061 accgaaatga tgcggccttc gacctcacgg tccggcgacc gcggcagaaa gttcatcggt
     6121 tgaacgtcct cttccgacat cagcaggttg ccgacgacgg cttcgcgctc ggtctcgagc
     6181 atgcgtaccg tgctcggatc gccggttcga tcgaggcggc cttggccgac gtagatgcct
     6241 tgatagccga gcacgtcctt ggtgtcggga tcgacgagct ctgcgccgcg ctcgacgacg
     6301 ttgaacaccg acccggtcgg cgcgtcggtg ccgcggacgt agacgtcgcg accggcgctg
     6361 ccgatcaaac cttcgcggtg cgccatgatg tacggcagct cgtcgacttc gtcctcttca
     6421 atgacgcgcg gccgcgacaa gaacgccgcg atcgtgtcgt acggaatcgt gttgattgct
     6481 tcctcgagcg gttcggagcg gacgcgaggc gacagtcgcg tcacgccgct accggcctgc
     6541 acgttttgcg gcccgcgctc gagctgcacg acgggccgcc cgtcgccgag gtacgcgagc
     6601 gacaagatat cgccgggaaa aatcaagtgc ggattctcga cctgcggatt gatctgcc
//
LOCUS       DATLEE010000612         6503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10807091, whole genome shotgun sequence.
ACCESSION   DATLEE010000612 DATLEE010000000
VERSION     DATLEE010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10807091"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1127
                     /gene="prfB"
                     /locus_tag="VM692_13315"
     CDS             join(32..103,105..1127)
                     /gene="prfB"
                     /locus_tag="VM692_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933612.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HUQ53198.1"
                     /translation="MEPNQIAPAIVALKERSAALRRYLDYDAKRERLEEVNRELENPA
                     VWSDSERAQNLGRERAALEAVVATLNRVEKSLDEAGELHELAAEEQDASTIDDVIGQV
                     KSVTKELEVLEFRRMFAGKMDSHNAFLDVQAGQGGTEAQDWAAMLLRMYLKWAERHGY
                     AVDVLEESAGEVAGIKSATIHVRGEYAYGWLRTELGVHRLVRKSPFDSGNRRHTSFAA
                     VFVSPEIEDDIEVELNPADLRVDVFRASGAGGQHVNKTESAIRITHIPTGIVVQCQNE
                     RSQHKNRATAMNQMRAKLYELEEQKRREQAQIVEDSKADIGWGNQIRSYVLDQSRVKD
                     LRTNFESARPQHVLDGDLDDFLQESLKQGL"
     gene            1138..2628
                     /gene="lysS"
                     /locus_tag="VM692_13320"
     CDS             1138..2628
                     /gene="lysS"
                     /locus_tag="VM692_13320"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HUQ53199.1"
                     /translation="MANDDEPENRLIAERRAKLARLREGGQAYPNDFRRDALADELLA
                     AYESHSAEQLAAAPLTVHVAGRLMAKRIGGGISFVRLQDRSGQIQLMIKRDRLGEAFY
                     GEFKKWDLGDVIGAVGQVVKSKTGELSVDVVGLKLLAKSLRPLPEKWHGITDAEMKLR
                     QRYVDLIMSAETRETFRRRSVLTRFIRSFLDALDFTEVETPMMQPIPGGATARPFVTH
                     HNALDATLYLRIAPELYLKRLLVGGFERIYELNRVFRNEGLSTRHNPEFTMLELYQAY
                     ANYRDLMSLTENLLRGAARALTGDTTVEYQGERFDLGAKFAEITVEAALERANPELAG
                     RVRDRAALASMCAARGIEVRPEHGAGKLQYDLFEKTVETTLKDPVFVTRYPAEVSPLA
                     RCNDEDPFVTDRFELFIGGRELANGFSELNDPEDQAARFRAQVEAKDAGDEEAMFFDA
                     DYIRALEYGMPPAAGLGIGIDRLAMLLTNSPSIRDVLLFPQLKPET"
     gene            complement(2625..3518)
                     /locus_tag="VM692_13325"
     CDS             complement(2625..3518)
                     /locus_tag="VM692_13325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HUQ53200.1"
                     /translation="MVLQHAALRDTVCLEVRGADAAPFLQAQLTRSVADLDPASAPLA
                     AWADPRGRLRALVRVCPLPDRWLLITPRDGADELLKRLRVFVLRAKVTLARADDVHVA
                     ALLGSADDWLVARGVAAADVAPNRLVRRGDVTLIRVGPAYWQVLGTSSAAHAFATELG
                     AAAEATEAELAEIALGIPAITLTLADRFVAQMLNLDALDAVSFDKGCYPGQEVIARVH
                     NLGGVKRRARRYAAPAEPPTVGTPVLAAAREVGEVVRSAPARTGCELLAIVEHAAVGS
                     PLTCAGAPLAELPLPFEVPRD"
     gene            3614..3814
                     /locus_tag="VM692_13330"
     CDS             3614..3814
                     /locus_tag="VM692_13330"
                     /inference="COORDINATES: protein motif:HMM:NF019511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HUQ53201.1"
                     /translation="MPDESGQRPVADAAPVTRPRAVDPQAPPGSRRGQPQVSQSVEIG
                     GPPGPEPTRFGDWERAGRCIDF"
     gene            3834..4211
                     /gene="sdhC"
                     /locus_tag="VM692_13335"
     CDS             3834..4211
                     /gene="sdhC"
                     /locus_tag="VM692_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HUQ53202.1"
                     /translation="MHGVQRPLSPYWIYRWEITMWLSSLHRITGLLLSLGAVVLAAWL
                     VALASGPEAFANVASIVGAPWFKVPLVGWTFCFFYHLANGVRHLAWDAGYGFDPRHIR
                     ASGRFVIIVTVLATAAFAYAAIL"
     gene            4222..4599
                     /gene="sdhD"
                     /locus_tag="VM692_13340"
     CDS             4222..4599
                     /gene="sdhD"
                     /locus_tag="VM692_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HUQ53203.1"
                     /translation="MSLMTPLNRVLGLGTAKGAGEHWWLQRVTAVALLPLGLWFAYGL
                     LTLPGFDYASVAAWVQRPVTSISLILLVVAVGYHSALGVQVVIEDYVTGGSKAATLIV
                     STLAHVGLTIAAVFAVLKIALGS"
     gene            4603..6390
                     /gene="sdhA"
                     /locus_tag="VM692_13345"
     CDS             4603..6390
                     /gene="sdhA"
                     /locus_tag="VM692_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HUQ53204.1"
                     /translation="MSKDYEFIDHTYDVVVVGAGGAGLRATLGLAEQGLSTACLSKVF
                     PTRSHTVAAQGGISAALGNMGPDDWRWHMYDTVKGSDWLGDQDAIEYMCKEAPASVIE
                     LEHYGVPFSRTEDGRIYQRPFGGMTTEFGKGIAQRTCAAADRTGHAMLHTLYQQSLKH
                     AAEFFIEYFAIDLIMENGACRGVTALDMATGRLHRYRAQMVILATGGYGRAYFSCTSA
                     HICSGDGNAMVLRAGLPLEDLEFVQFHPTGIYGAGCLITEGSRGEGGYLTNSAGERFM
                     ERYAPNAKDLASRDVVSRSITIEIREGRGVGKHKDHVHLHLEHLGRDVIDERLPGIRE
                     TAKIFAGVDVLKAPIPVLPTVHYHMGGVPTNYHGEAFTKDAKGNEQIVPGLMAIGEAA
                     CVSVHGANRLGSNSLIDLVVFGRAAAERCGKLIERGSRPRPLGAGAGDEAIARLDKLR
                     NAKGKTPTAAIRLEMQRVMQAHAAVFRTGESLQEGIDQLEQVFASFGDVAVSDRSLIW
                     NTDLVETLELDNLLLQAMGTIKSAANRTESRGAQAREDYPERDDVNWMKHTLCWVDAR
                     GAVRFDYKPVTMSTLTDEVKAIPPKARTY"
BASE COUNT         1148 a         2106 c         2207 g         1042 t
ORIGIN      
        1 tctgcgcccg aaaagcgatc gcctcgtgca aatggaaccg aaccaaatcg cgcccgccat
       61 cgtcgcgctc aaagagcgtt ccgccgcgct gcggaggtat ctttgactat gacgccaagc
      121 gcgagcggct cgaggaagtc aaccgcgagc tagaaaatcc cgccgtctgg tccgactccg
      181 agcgcgcgca aaacttgggc cgcgagcgcg cggcgctcga ggccgtcgtc gcgacgctga
      241 atcgcgtcga gaagtcgctc gacgaggccg gcgagctgca cgagcttgcc gcggaagagc
      301 aagacgcgag cacgatcgac gacgtcatag gccaagtgaa gagcgtgacg aaggagctcg
      361 aggtgctcga gttccgccgc atgttcgccg gcaagatgga ctcgcacaac gcgtttctcg
      421 acgtccaggc cggccagggc ggcaccgaag cccaggactg ggccgcgatg ttgttgcgca
      481 tgtatttgaa gtgggccgag cggcacggct acgcggtcga cgtgctcgag gagtcggccg
      541 gcgaggtcgc cggcattaag agcgcgacga tccacgtccg cggcgagtac gcctacggtt
      601 ggttgcggac ggagctcggc gtgcaccggc tcgtgcgcaa gtcgccgttc gattcgggca
      661 atcgacggca tacgtcgttc gcggccgtgt tcgtgtcgcc ggagatcgaa gacgacatcg
      721 aggtcgagct gaatcccgcc gacctgcgcg tcgacgtgtt tcgcgccagc ggcgccggcg
      781 gtcagcacgt caacaagacg gagtcggcga tccgcatcac gcacattccg accggcatcg
      841 tcgtgcagtg ccagaacgag cgctcgcagc acaagaaccg cgcgacggcg atgaaccaga
      901 tgcgcgcgaa gctctacgag ctcgaggaac agaagcgcag ggagcaggcg cagatcgtcg
      961 aggacagcaa ggccgacatc gggtggggca accagatccg gtcctacgtt ctcgatcagt
     1021 cacgcgtgaa ggatttacgc acgaacttcg agagcgctcg gccgcagcac gttctcgacg
     1081 gcgacctcga cgactttctg caggaaagct tgaagcaggg cttgtaagag acgagcgatg
     1141 gcaaacgacg acgaaccgga aaatcggctc attgcggagc ggcgcgcgaa gctcgcgcgg
     1201 ctgcgcgagg gcggccaggc atatccgaac gactttcgcc gcgacgcgct cgccgacgag
     1261 ctgctcgcgg cgtacgagtc gcacagcgcc gagcagttgg cggcggcgcc gctgaccgtg
     1321 cacgtcgccg ggcggctcat ggccaagcgc atcggcggcg gcatcagctt cgtgcggctc
     1381 caggatcgct cgggccagat tcagctcatg atcaagcgcg accgtctcgg cgaggcgttc
     1441 tacggcgagt tcaagaagtg ggacttgggc gacgtcatcg gcgctgtcgg tcaagtcgtc
     1501 aagagcaaga ccggcgagct gtccgtcgac gtggtcgggc tcaagctgct cgcgaagtcg
     1561 ctgcggccgc tgcccgagaa gtggcacggc atcacggatg ccgagatgaa gctgcgccaa
     1621 cgctacgtcg atctcatcat gagcgcggag acgcgcgaga cgttccgccg ccgcagcgtg
     1681 ctcacgcgct tcatccgcag cttcttggac gcgctcgact tcacggaggt cgagacgccg
     1741 atgatgcagc cgatccccgg cggcgcgacg gcacggccgt tcgtgacgca ccacaacgcg
     1801 ctcgacgcga cgctctattt gcgcatcgcg cccgagctgt acttgaagcg gttgctcgtc
     1861 ggcggcttcg agcgcatcta cgagctgaac cgcgtgttcc gcaacgaggg cttgtcgacg
     1921 cgccacaacc ccgagttcac gatgctcgag ctctatcagg cttacgcgaa ctatcgcgat
     1981 ttgatgtcgt tgaccgaaaa cctgctgcgc ggagcggcgc gggcgctgac gggtgacacg
     2041 acggtcgagt accagggcga gcgtttcgac ctcggcgcca agttcgccga gatcactgtc
     2101 gaagccgcgc tcgagcgcgc gaacccggag ctcgcgggac gcgttcgcga tcgcgccgcg
     2161 cttgcgagca tgtgcgccgc ccgcggtatc gaagttcggc ccgagcacgg cgccggcaag
     2221 ctgcaatacg acttgttcga gaaaacggtc gagacgacgc tcaaggatcc cgtgttcgtg
     2281 acgcggtatc cggccgaggt ctcgccgctc gcgcgttgca acgacgaaga tccgttcgtg
     2341 acggatcggt tcgagctgtt catcggcggc cgcgagctcg cgaacgggtt ctccgagctc
     2401 aacgatcccg aggaccaagc ggcgcggttt cgcgcacagg tggaagccaa ggacgccggc
     2461 gacgaggagg cgatgttctt cgacgccgac tacatccgcg cgctcgagta cggcatgccg
     2521 ccggccgctg ggttgggtat cggcattgac cggctcgcga tgctgctgac gaactcgccg
     2581 tcgatccgcg acgtcttgct gttcccgcag ctgaagcccg agacttagtc gcgcgggacc
     2641 tcgaagggta gcggcagctc cgcgagcggc gcgccggcgc acgtcaacgg cgaacctacg
     2701 gccgcgtgct cgacgatggc cagcaactcg caacccgttc gcgccggcgc gctgcgaacg
     2761 acttcgccga cttccctggc ggccgcgagc acgggcgtgc cgacggtcgg aggctcggcc
     2821 ggcgcggcgt agcgccgcgc tcggcgtttg acgccgccca agttgtgcac gcgcgcgatg
     2881 acttcctgcc ccggatagca gcccttgtcg aacgacacgg catcgagcgc gtcgaggttc
     2941 agcatttggg cgacgaaacg gtcggcgagc gtcagcgtga tcgcgggaat gccaagcgca
     3001 atttccgcga gctcggcctc ggttgcttcc gccgccgcgc cgagctccgt cgcgaacgcg
     3061 tgcgcagcgc tcgacgtgcc gagcacctgc cagtaggcag gaccgacgcg aatcaacgtc
     3121 acgtcgccgc ggcgcaccag tcggttcggt gctacgtcgg ccgccgcgac gccgcgcgca
     3181 acgagccagt cgtccgccga gccgagcagc gctgcgacgt ggacgtcgtc ggctcgcgcg
     3241 agcgttacct ttgcgcgcag cacgaacaca cgcagcctct tcaagagctc gtcggcgccg
     3301 tcgcgcggcg tgatcagcag ccagcggtcg ggtaggggac agactcgaac cagcgcgcgt
     3361 aagcgcccgc gcggatcggc ccaagccgcg agcggtgcgg aagcggggtc gaggtccgcg
     3421 accgagcggg tcagctgtgc ctgtagaaaa ggggctgcat cggcgccgcg cacctctaag
     3481 cagacggtgt cgcggagcgc cgcgtgctgc aaaaccatcg tttcgtccgt cgtgagcttt
     3541 catcaccgcg gaatgtcgcg gtgcacaacc gtgtcgtagt gcggagtcta ggggtttctg
     3601 gcaaaatttg ccgatgcctg atgagagcgg tcaacggccg gtcgccgacg cggcaccggt
     3661 aacacggcca cgcgccgttg atccgcaagc gccgccaggc agccggcgcg gccaacctca
     3721 agttagccag agtgtcgaga tcggcggccc gccggggccc gagccgacgc gcttcggcga
     3781 ttgggagcgc gcggggcgct gcatcgattt ctaacgtgag ggagcagggc gaaatgcacg
     3841 gggttcagcg gccgctgtcg ccgtactgga tttatcgttg ggagatcacg atgtggctgt
     3901 cgagcctgca tcggatcacg gggctgttgc tatcgctcgg cgccgtcgtg cttgcggcat
     3961 ggttggtcgc gctcgcaagc ggtcccgaag cgttcgccaa cgttgcatcg atcgttggcg
     4021 cgccttggtt caaagtgccg ctggtcggct ggacattttg tttcttctat cacctcgcga
     4081 acggcgtgcg tcaccttgcg tgggacgcag gttacggctt cgatccgcgg catattcgcg
     4141 cgagcggtcg gttcgtcatc atcgtgacgg tcctcgcgac ggcggcgttc gcctacgccg
     4201 cgattctcta gggctgcgcc aatgagcttg atgaccccgc tgaatcgcgt cctcggcctc
     4261 ggcacggcca aaggtgccgg cgagcactgg tggttgcagc gcgtgaccgc tgtcgcgctg
     4321 ctgccgctcg gcttgtggtt cgcctacggg ttgctgacgc tgcccggctt cgactacgcg
     4381 tcggtcgcgg cctgggtgca acgaccggtc acgagcatct cgctgatcct gctcgtcgtg
     4441 gccgtcggtt atcactcggc actcggcgtg caagtcgtca tcgaagacta cgtgacgggc
     4501 ggctcgaaag ccgcgacgct gatcgtgtcg acgctcgcgc acgtcggtct gacgatcgcg
     4561 gccgtgttcg ccgtgctgaa aatcgcgctt ggatcctagg caatgagcaa agactacgaa
     4621 ttcatcgatc acacctacga cgtggtcgtc gtcggcgcgg gcggcgccgg attgcgcgcg
     4681 acgctcggcc tcgccgagca agggctctcg acggcgtgct tgagcaaagt gttcccgacg
     4741 cgcagtcaca ccgtcgcggc gcaaggcggc atcagcgccg cgctcggcaa catgggcccc
     4801 gacgactggc gttggcacat gtacgacacg gtcaagggct ccgactggct cggcgaccag
     4861 gacgcgatcg agtacatgtg caaggaagcg cccgcgtccg tcatcgagct cgagcactac
     4921 ggcgtgccgt tttcgcgcac cgaggacggc cgcatctatc agcgcccgtt cggcggcatg
     4981 acgacggagt tcggtaaggg catcgcccag cgcacctgcg ccgctgccga tcgcacgggc
     5041 cacgcgatgc tgcacacgct gtatcagcaa tcgcttaagc acgcggccga atttttcatc
     5101 gagtacttcg cgatcgacct catcatggag aacggcgcgt gccgcggcgt caccgcgctc
     5161 gacatggcga cggggcggtt gcaccgctat cgcgcgcaga tggtcatcct cgcgacgggc
     5221 ggctacggcc gcgcctactt ctcgtgcacg tcagctcaca tctgctcggg cgacggcaac
     5281 gcgatggtgc tgcgagccgg cctgccgctc gaggacctcg agttcgtgca gttccacccg
     5341 accggcatct acggcgccgg ttgtcttatc acggaaggct cgcgcggcga gggcggctat
     5401 ctcacgaact cggcaggcga gcgcttcatg gagcgctacg cgccgaacgc gaaggacctc
     5461 gcatcgcgcg acgtcgtgag ccgctcgatc acgatcgaga ttcgcgaagg gcgcggcgtc
     5521 ggcaaacaca aagaccacgt gcacctgcac ctcgagcatc tcggccgcga cgtcatcgac
     5581 gagcgcctgc cgggcatccg cgaaaccgcc aagatttttg ccggtgtgga cgttttgaag
     5641 gcgccgatcc ccgtcttgcc gaccgtgcac taccacatgg gcggcgtgcc gacgaactat
     5701 cacggcgaag cgtttacgaa ggatgcgaaa ggcaacgagc agatcgtgcc gggcctcatg
     5761 gcgatcggcg aggccgcgtg cgtctccgtg cacggcgcga accggcttgg ctcgaactcg
     5821 ctgatcgacc tcgtcgtctt cgggcgcgcg gcggcagaac gttgcggcaa gctcatcgaa
     5881 cgcggctcgc ggccgcggcc gctcggcgcg ggtgccggcg atgaagcgat cgcacggctc
     5941 gacaagctgc gcaacgccaa aggcaagacg ccgacggccg cgatccgtct cgagatgcag
     6001 cgcgtcatgc aggcccacgc ggccgtcttc cgcacgggcg agtcgctcca ggaaggcatc
     6061 gatcagctcg agcaagtatt cgcgtcgttc ggcgacgtgg ccgtcagcga tcgctcgctg
     6121 atctggaaca cggatctcgt cgaaacgctc gagctcgata acctgcttct gcaagcgatg
     6181 ggcacgatca agtccgcggc gaaccgcacg gagagccgcg gcgctcaggc gcgcgaggat
     6241 tatcccgagc gcgacgacgt caattggatg aagcacacgc tgtgttgggt ggacgcgcgc
     6301 ggtgccgtgc gcttcgacta caagcccgtg acgatgagca cgctcacgga cgaggtcaaa
     6361 gcgattccgc cgaaggcccg cacgtactga tcgcgcacga cgaaggatga aatagggaat
     6421 cacgatggcg cagttcacgc ttccagcgaa ctccaaggtc aagcccggca agaaccatgg
     6481 cggcgccgca ggcgctgcca acg
//
LOCUS       DATLEE010000613         2685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7077833, whole genome shotgun sequence.
ACCESSION   DATLEE010000613 DATLEE010000000
VERSION     DATLEE010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7077833"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VM692_13350"
     CDS             <1..950
                     /locus_tag="VM692_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53205.1"
                     /translation="ARLQNVARELLAYFTRKRGVTSQIGVFATPDGVAAAQVTPRANG
                     KPRLDRCVYEKDAAPDAFTRALDRLPSRRAPTVTVLDPAAYRLLLVEAPEVPADELRA
                     AVRWRVKDLIDFHIDDAVIDVFEMPQHARGGTRRMMYAVTAKADVVKQAIERIEGAGL
                     ELEVVDIPELSLRNVATLLEAEQRGAALLYLGERRSTLLLVRQGILYLARHMETGVAT
                     LAEAGELRADLVAGLALEVRRSLDHFESHYEQTSIQQLHVSGLEAADRDAIARELALN
                     VREVDLRALFETDDELSPELQRLALPAIGAALRKDAVAL"
     gene            954..1580
                     /locus_tag="VM692_13355"
     CDS             954..1580
                     /locus_tag="VM692_13355"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HUQ53206.1"
                     /translation="MYQQINLYQPIFRKQRQIFSAVTMLQAVGVVAAALLAIYGYGLA
                     KVGTLEAEVVQLEGREKALTTQLMRIDPKMSANRRAELETELERLNATLLDQQRLIDV
                     LRDRPLGDSAGFSGYLAALARQRTPELWLTEIGINGGTAAIELAGRSLDPKLVPEYMQ
                     RLGTEPALAGQQFDRFEIALDDETHESTFRATSRAALRAADNGSGRRP"
     gene            1577..2269
                     /locus_tag="VM692_13360"
     CDS             1577..2269
                     /locus_tag="VM692_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53207.1"
                     /translation="MRERLSNLQQSVDRLSLRERLLVLGAALFIVGGLWEAVLAAPLA
                     AREQIANDKVSALQSRLVELDAALSAAAAGLSEGMPDQLARLQALRASVQERDDEMRV
                     FTTDLVDPAQMRVMLEELLRRQGGLTLVSAVNLPARAVLDDDGEDAPTADVASGDAAE
                     RSGAPDLYRHSFVLKLRGDYLDCLRYLEDVERLPWHIYWSRLELRTDEYPVNDIVIEL
                     ATLSLDEEWIGV"
     gene            2262..2618
                     /locus_tag="VM692_13365"
     CDS             2262..2618
                     /locus_tag="VM692_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53208.1"
                     /translation="MSKAAPFVVALLAIAAARAEDTPPVRDPMRPFGSVAAAAAAAAK
                     APRFAMTAVLIAPSRRVAIVNGKPYAQGATVDGAEIVAIEHDVVRLRERGAELVIHLG
                     RPGDGRPQSVQGETVP"
BASE COUNT          432 a          900 c          932 g          421 t
ORIGIN      
        1 tagcgagatt acagaacgtg gctagagaat tgttggcgta tttcacgcga aagcgggggg
       61 taaccagcca gatcggcgtc ttcgcgacgc cggacggtgt ggcggctgct caagttacgc
      121 cgcgcgccaa cggcaagccg cggctcgatc gctgcgtcta cgagaaggac gccgcgccgg
      181 acgcattcac gcgcgcgctc gaccgattgc cgagccgccg cgcgccgacg gtcacggtgc
      241 tcgatcccgc cgcgtaccgt ttgctgctcg tcgaggcgcc cgaggtgccg gccgacgagc
      301 tgcgcgccgc cgttcgctgg cgcgtcaagg atctcatcga cttccacatc gacgacgccg
      361 tcatcgacgt gttcgagatg ccgcagcacg cacgcggcgg cacgcgccgc atgatgtacg
      421 ccgtcacggc gaaggccgac gtcgtcaagc aagcgatcga gcgcatcgaa ggcgcggggc
      481 tcgagctcga ggtcgtcgac attcccgagc taagcctgcg caacgtcgcg acgctgctcg
      541 aggccgagca acgcggcgcg gcactgctgt acctcggcga gcggcgcagc acgttgctgc
      601 tcgtgcgcca gggcatcctg tacttggccc gccacatgga gaccggcgtc gcgacgctgg
      661 ccgaggccgg cgagctgcga gccgatctcg tcgcggggtt ggcgctcgaa gtgcggcgct
      721 cgctcgacca tttcgagagc cactacgagc agacctcgat tcagcagctg catgtgagcg
      781 gcctcgaggc cgccgatcgc gacgcgatcg cgcgtgagct cgctttgaac gtacgtgagg
      841 tcgacttgcg ggcgctgttc gagaccgacg acgagctgtc gcccgagctc caacggctgg
      901 cgttgcccgc gatcggtgcg gcgctgcgca aagacgccgt cgcactctag gccatgtacc
      961 agcaaatcaa cctctatcag ccgatcttcc gtaagcagcg gcagatcttt tcggccgtca
     1021 cgatgctgca ggccgtcggc gtcgtcgccg cggcgctgct cgcgatctac ggctatgggc
     1081 tcgcgaaggt cggcacgctc gaggccgagg tcgtgcagct cgaaggccgc gagaaagcgt
     1141 tgacgacgca gctcatgcgc atcgatccga agatgagcgc gaatcggcgc gccgagctcg
     1201 aaacggagct cgagcgcttg aatgccacgc tgctcgatca gcaacggctc atcgacgtgc
     1261 tgcgcgaccg gccgcttggc gactcagcgg gtttctcggg ttatctcgcg gcgctcgcgc
     1321 gccagcgcac gccggagctc tggctcacgg agatcggcat caacggcggc acggccgcga
     1381 tcgagctcgc cggcagaagc ctcgatccca agctcgtgcc cgagtacatg cagcggctcg
     1441 gcaccgagcc cgcgctggcg ggccagcagt tcgaccgctt cgaaatcgcg ctcgacgacg
     1501 agacgcacga gtcgacgttc cgcgcgacta gccgcgcggc gctgcgagcg gccgacaacg
     1561 gcagcggacg gcggccatga gggagcgcct ctcgaacctt cagcaaagcg tcgaccgctt
     1621 gagcttgcgc gagcggctac tcgtcctcgg cgcggccttg ttcatcgtcg gcgggctctg
     1681 ggaagccgtg ctggctgcgc cgttggcggc gcgcgagcag atcgcgaacg acaaggtctc
     1741 ggcgctgcag tcgcgcttgg tcgagctcga cgcggcgttg agcgcggcag ccgccggtct
     1801 cagcgaaggc atgccggacc agctcgcacg gttgcaggcg ctgcgcgcga gcgtgcaaga
     1861 gcgcgacgac gagatgcgcg tcttcacgac cgacctggtc gatccggcac aaatgcgtgt
     1921 aatgctcgag gagctgttac gccggcaagg cggcctcacg ctcgtcagcg ccgtcaacct
     1981 gccggctcgg gcggtgctcg atgacgacgg cgaagacgcg ccgaccgccg atgtcgcgag
     2041 cggcgacgcg gccgagcggt cgggcgcccc cgatctgtat cgccattcgt tcgtgctgaa
     2101 gctgcgcggc gattacctcg actgcctgcg ctacctcgag gacgtcgagc gtttgccgtg
     2161 gcacatctac tggtcgcggc tcgagcttcg gaccgacgag taccccgtca acgacatcgt
     2221 catcgagctc gcgacgttga gtttggacga ggagtggatc ggtgtctaag gcggccccct
     2281 tcgtagttgc gttgctcgca atcgccgccg cgcgcgctga ggacacgccg cccgtacgcg
     2341 acccgatgcg gccgttcggc agcgtcgccg cagcggccgc agccgcggcg aaggccccgc
     2401 ggttcgcgat gacggccgtg ctgatcgcgc ccagtcgacg cgtcgcgatc gtcaacggca
     2461 agccgtacgc ccaaggcgcg accgtcgacg gtgccgagat cgtggcgatc gagcatgacg
     2521 tcgtgcgttt acgcgagcgc ggcgctgagc tcgtgattca tcttggcagg ccgggcgatg
     2581 gacggccgca gagcgtgcaa ggagagacgg ttccatgact ttaaaactcg caagccccgc
     2641 ggtgctcgcg ctggccgccg gcctgtgtct cgcggctggt tgcgc
//
LOCUS       DATLEE010000614         2728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10809200, whole genome shotgun sequence.
ACCESSION   DATLEE010000614 DATLEE010000000
VERSION     DATLEE010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10809200"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..754
                     /locus_tag="VM692_13370"
     CDS             44..754
                     /locus_tag="VM692_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53209.1"
                     /translation="MHKLAIAALATATVGGAATVVVVAASRNDAHADTHTTVSRATAH
                     PESAPPHASHGAHGGALCDVMEKLHGHKSAASRSPLAALTATADANDCSAVGEHLADL
                     QADATHGPNSRPDETSFETCSSTYAAMCESEHWSEERRACTLGAGDLINAHLCAGHVA
                     TSEQPPAAVPANLTCSVLGPQIASTIQAAGMHDDVTDLGEQIAAACDVGNWTVELRTC
                     FSTASTVDALKTCIGHEE"
     gene            complement(758..1525)
                     /locus_tag="VM692_13375"
     CDS             complement(758..1525)
                     /locus_tag="VM692_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53210.1"
                     /translation="MSRTNGHLLLCCSLAVTIAGTAHADVYPRPVIARPLTLPANVAS
                     LGADAGGNHDLSAVSAAPIAGYGITDKLEVQVPYAFAVHDLEARGSVAVDAGYAVVRG
                     AFDGKLEVIERIRVGYDALASSAMPLQIGLHAQYNVKPWLAIISGTPGSEQLRISLTN
                     NVDMMKPIDIGLPLGVGVQPSETVYLQLDTKLVQVGLSESETLVVVRDIVPLALTVVW
                     NVLPVLDVQAAVATDLRNAPADALAFLVGARFYAGRL"
     gene            1691..>2728
                     /locus_tag="VM692_13380"
     CDS             1691..>2728
                     /locus_tag="VM692_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HUQ53211.1"
                     /translation="MLFGLVGAAVADDAASAPSVEQRLANLEAYVTNGVPDPHGGALA
                     ATPGPGHNGWMMISTALVLFMTLPGLGLFYGGLVRRKNVLSVLAQCFGCAGLVTILWW
                     AIGYSLVFARGNAVIGGFDFAMFNGVTAAPNADYGAWVSQNVFAMFQMMFAIITPALI
                     VGAIAERMKYSAVIAFITLWMFVVYFPLAHMVWGIDGLMNGVWNAKASIKSIDFAGGT
                     VVHMSSGWSALVLCLILGKRTGFGKRAFLPHSLVLTVVGTGMLWVGWYGFNAGSAVAA
                     DGIAANAFVTTTLAAAVAGAVWPTLEWATRGKPSVLGFCSGVVAGLVVVTPAAGYVSA
                     SSAILIGLLAGV"
BASE COUNT          450 a          920 c          895 g          463 t
ORIGIN      
        1 ttcgcatgtc gatgcctcac ccgctgctgc gaaaggatcg atcatgcaca agctcgccat
       61 tgccgctctc gccactgcca ccgtcggagg tgctgcgact gtcgtcgtcg tcgccgcatc
      121 gcgcaacgat gcacacgccg acacgcacac gactgtctcg cgcgcgaccg cacacccgga
      181 gtccgcgccg ccacacgcct ctcacggcgc gcacggtgga gcgctctgcg atgtgatgga
      241 gaagctgcac ggccacaagt ctgccgcgtc gaggtcaccg ctcgccgcgc tcaccgcgac
      301 ggccgacgcg aacgactgca gcgccgtcgg cgagcacctc gccgatctgc aggcggacgc
      361 gacgcacggc ccgaacagcc gccccgacga aacgagcttc gagacctgca gctccaccta
      421 cgcagcgatg tgcgaatcgg agcactggtc ggaagaacgc cgcgcgtgca cgctcggcgc
      481 gggcgacctg atcaatgcgc atctgtgcgc cggtcacgtc gcgacgtccg agcagccgcc
      541 cgccgccgtt ccggcaaacc tcacgtgcag cgtgctcggc ccgcagatcg catcgacgat
      601 ccaggcggcc ggcatgcacg acgacgtgac cgacctcggc gagcaaatcg ctgctgcatg
      661 cgacgtcggg aactggacgg tcgagctgcg cacatgtttt tcgacggcga gcaccgtcga
      721 cgcgctcaag acctgtatcg gccacgagga gtagccgtca cagccgtccc gcgtagaagc
      781 gcgcaccgac gagaaaggcg agcgcgtccg caggtgcgtt gcgcagatcg gtcgcgacgg
      841 ccgcctgcac gtcgagcacg ggaagcacgt tccacacgac cgtcagcgcg agcggcacga
      901 tgtcgcgaac gaccacgagc gtctcggact ccgagagccc gacctggacg agcttcgtgt
      961 cgagctgcaa gtagacggtc tccgatggct gcacgccgac gccgagcggg agaccgatgt
     1021 cgatcggctt catcatgtcg acgttgttcg tgagcgagat gcgcagttgc tcgcttcccg
     1081 gtgtgcccga gatgatcgcg agccacggct tcacgttgta ctgcgcgtgc agaccgatct
     1141 gcagcggcat cgcagagctc gcgagcgcgt catagccaac gcgaatgcgc tcgatcacct
     1201 cgagcttgcc gtcgaaggcg ccgcgcacga ccgcgtatcc ggcgtcgacg gcgacgctgc
     1261 cgcgcgcttc cagatcgtgc acggcgaacg cgtacggcac ctggacctcg agcttgtccg
     1321 tgatgccgta gccggcgatc ggagccgcgc tcaccgcgct cagatcgtga ttgccgccgg
     1381 catcggctcc gagcgacgcg acgttcgccg gcaaggtcag cggacgcgcg atcaccggac
     1441 gcggatacac gtccgcgtgc gcagtccccg cgatcgtgac ggcgagagaa caacaaagaa
     1501 gaagatggcc gttggttctc gacatggacc cccagcctga cgggtccacg tttcgtgaac
     1561 gttgctccaa cgtttcgtta acgcgacgcg caaaggttta gcgagcgcga tacgcgttgg
     1621 aaacaatcac ctcgctaccg tctggtggat gacacgccat cggatcgcag gagcaatcgt
     1681 tctcgggttg atgctgtttg gcctcgtcgg tgcggcggtt gcggacgatg cagcgtcggc
     1741 gccgagcgtc gagcagaggc tcgcgaacct cgaagcgtac gtcaccaacg gcgtccccga
     1801 tccccacggc ggggcgctcg cggcgacgcc ggggcctggc cacaacggct ggatgatgat
     1861 ctcgaccgcg ctcgtgctgt tcatgacgtt gccggggctt ggcctcttct atggcgggct
     1921 cgtgcgtcgc aagaacgtgc tgtcggtcct agcgcagtgc ttcggctgcg ccgggctcgt
     1981 cacgatcctg tggtgggcga tcggctacag cctcgtgttc gcgagaggta acgcggtcat
     2041 cggcggcttc gacttcgcga tgttcaacgg cgtcacggcc gcacccaacg ctgactacgg
     2101 cgcgtgggtg tcacagaacg tgttcgcgat gttccagatg atgtttgcga tcatcacgcc
     2161 cgcgctgatc gtcggtgcga tcgcggagcg catgaagtac tcggccgtga tcgcgttcat
     2221 cacgctctgg atgttcgtcg tctacttccc gctcgcgcac atggtgtggg gcatcgatgg
     2281 gctcatgaac ggcgtctgga atgccaaggc atccatcaag tcgatcgact tcgcaggcgg
     2341 cacggtcgtg cacatgtcgt caggctggtc ggcgctcgtg ctctgtctca tccttggcaa
     2401 gcgcaccggc ttcggcaagc gcgcattcct cccgcacagt ctcgtgctca ccgtcgtcgg
     2461 caccggcatg ctctgggtcg gctggtacgg cttcaacgca ggcagcgcgg tggccgccga
     2521 cggcattgcc gccaacgcgt ttgtcacgac gactctcgcc gcagcagtcg ccggcgcggt
     2581 gtggccgacg ctcgagtggg cgacgcgcgg caagcccagc gtgctcggat tctgctcggg
     2641 cgtcgtcgct ggtctcgtcg tcgtcacgcc cgctgcgggt tacgtgtcgg cgagcagcgc
     2701 gatcctgatc ggcctcctgg ccggcgtc
//
LOCUS       DATLEE010000615         5851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2225474, whole genome shotgun sequence.
ACCESSION   DATLEE010000615 DATLEE010000000
VERSION     DATLEE010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225474"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2340
                     /locus_tag="VM692_13385"
     CDS             <1..2340
                     /locus_tag="VM692_13385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ53212.1"
                     /translation="ALGIGVNTAIFTIVNSVIFKGMPFDNPAEIAYISSNRGAISYPD
                     FVDFRAQSRSFKGLAAFTNLGADLSDGDAAAERVSGARVTANAFSLLGAEPLIGRDFT
                     TEDEQRGADPVALLSYGLWQARYNGDANILGRTIRINLADYTVIGVMRPGEAFPNDTR
                     LWIPYIPDAAAQRRDARGLLGFARLGDGVSYERAGSELLTLTQGLAQAYPDTNAEIET
                     RIAPYTDRGTTGPIRVILYSLLGAVCFVLLIACANVANLLLSRAIQRTRETSIRNALG
                     ASRWRVVRQLLVESVMMSVLGGVLGLGVGLLAVRWIDVATQPTGRPYWLDFSMDYRVF
                     FYFAAVSVVTGILFGLAPALQISKSNVSENLKEGGRSGFSGRRAGKMTDALLVGQIGL
                     TIVLLVGAGLMIRSFLITQQFDIGVNTENLVTVQVQPPNARYPQPADRLAFQERLAER
                     LEALPGVDSLTIVSQPPAGGAQMRTLKIEGREMTDANNRLPLVGRLVIGPDYFDSLEL
                     TIRRGRGFTDNDGASGAEAVIVNETFVTRYFPDTDPIGSRLRLGGDLDRGTEDVTAPW
                     LTIVGVSPPVFQQSPNQDLSVQPTAYVPFRQEPPVAFTVLARSRVAGDSVVTAIRNEL
                     RQIDPDLPLYNIRTLDEILAQRNWPYRIFGTLFGTFAVIALLISSVGIYAATAHGIGQ
                     RRQELGVRMALGAGRRDILWLVLRQGIARIAIGVVLGVLAAVGVSRVLVSVLVNTTAT
                     DPTTFVAICLLLAAVTLLACFLPARRATRLDPVDALRTE"
     gene            2342..3868
                     /locus_tag="VM692_13390"
     CDS             2342..3868
                     /locus_tag="VM692_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUQ53213.1"
                     /translation="MTRLAMSLAALAACGSAAGGVLHEQAERAAQRNFGEYLDALRIP
                     NVADKPADIARNAAFFSRAFERRGFDVRLVTNAAGRPVVLAELPARAGLPTVLLYIHY
                     DGQPIVPAEWAQADPFMPVVRRRNAAGAWQDVETNALQAQPLDPELRVFARSAADDKA
                     PIMMLLTAFDVLATQAEAPAYNVKVMLDGEEEMGSPSLAATVASERARFAADALVILD
                     GPLHASGRPTVVFGNRGIAQATLTVFGPRAPLHSGHYGNYAPNPALRLAALLASMKDD
                     DGRVLIPGYYDGVRLTEADRITLGDVGDDEAALRARIGIARAESVGANYQESLQYPSL
                     NVRGIAAGGVGAVATNSVPSQAVAELDMRTTPETDGRRLYELVRAHIERAGYHLIDAE
                     PTDAERARFDKLARFTLGSTQAAMRMPLDSTVGRWANMALRAPSAPQPGEAPVQIRMM
                     GGTVPTDVLIAALQLPFVLLPLVNDDNNQHAPNENLRIGNFVTGTEALYSLLVTPYPR
                     "
     gene            4051..5478
                     /locus_tag="VM692_13395"
     CDS             4051..5478
                     /locus_tag="VM692_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53214.1"
                     /translation="MRTIQSLALAASFVAAAPVFAQKMPDIGFKSVGRGAPLAASVYD
                     YKQEVGPNWIRTQGQQAGPDVKKPFPLNGYLPSELPKDYKPLPRDIYTSDDFYKDKEL
                     WSDPRYFRCNSPQATEYQRGVLQRPGVNTSDKIEDAPWGHCDISLPREAIVSPYGFKT
                     AHEHYEALKAETTKRGGPNVYTYENFPNAEWNGVYQRPGGANGAGAANQNNWYWGRHT
                     QIPTVLSVLTPKYQEYMVQEAYHQVRGNALWPSTFCWPEGFMRRWYPAAVSEHYIIAT
                     PDLVHVRTGVARNFIQDTYIGREFNMEDVPKGGVPRLGPAVPRWYGDTIGFWDKDTLI
                     TWTSNIQGWKSHSEFEFSSQMQSIEIYTPIREGGKFIGLNHETILYDSEALATPVRIV
                     RNLHKINNFTDTNEVPYAFIECVQTIFNVNGSNTPLAPGAKLEYEMPDMYGRPWAKIW
                     KESFEQNMSGPADTSEDIFNFEDNK"
     gene            5525..>5851
                     /locus_tag="VM692_13400"
     CDS             5525..>5851
                     /locus_tag="VM692_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53215.1"
                     /translation="MATNNGFGLAAGAVAVAAALCTTALAQQSPQIDFKSVGRGAPVM
                     VDAAALPVVGATYQGGGGPGGNGQPQTPRTFVGSAEPGQSPKGIKPLPRDMFTSDDFY
                     KDKDLWS"
BASE COUNT         1010 a         1955 c         1936 g          950 t
ORIGIN      
        1 gcgctcggca tcggcgtgaa tacggcgatc ttcacgatcg tcaattccgt gatcttcaag
       61 gggatgccgt tcgacaatcc cgccgagatc gcttacatct cgtcgaatcg cggcgccatt
      121 tcgtaccctg atttcgtcga ctttcgcgcg cagagccgct cgttcaaagg gcttgccgca
      181 ttcacgaatc tcggcgccga tctgagcgac ggcgacgccg ccgccgaacg cgtgagcggc
      241 gcgcgcgtca cggcgaacgc tttctcgctg ctcggcgccg agcctttgat cggcagagat
      301 ttcacaactg aggacgagca gcgaggcgcc gatcccgtgg cgctgttgag ctacggtctt
      361 tggcaagcgc gctacaacgg tgacgcgaac atcctcggcc ggacgatccg catcaatctc
      421 gcggactaca ccgtgatcgg cgtcatgcgg cccggcgagg cgtttccgaa cgacacgcgc
      481 ctgtggattc cgtacattcc cgacgcggca gcgcaacgcc gcgacgcgcg cgggctcctg
      541 gggttcgcac gcctcggcga cggtgtctcg tatgagcgcg cgggaagcga gctgttgacg
      601 ctgacgcaag ggttggcgca agcgtatccc gacacgaacg cggagatcga gacgcgcatt
      661 gcgccgtaca ccgatcgcgg cacgacgggg ccgatacgcg tgattctcta ttcgctgctc
      721 ggtgccgtct gcttcgtgct cctcattgcg tgcgccaacg tcgctaactt gctgctgtcg
      781 cgcgcgattc aacgcacgcg cgagacgtcg attcgcaacg cgctcggtgc gagccgctgg
      841 cgcgtcgtgc ggcagctgct cgtcgagagc gtgatgatga gcgtgctcgg cggagtgctc
      901 gggttgggcg tcggcttgct cgcggtgcgc tggattgacg tcgcgacgca gccgaccggc
      961 aggccttact ggctcgattt ttcgatggac tatcgcgtgt tcttctactt cgcggccgtc
     1021 tcggtcgtga cgggcatctt gtttggcctc gctccggcct tgcagatctc gaagtcgaac
     1081 gtcagcgaga acttgaagga aggcggccgc agcggcttca gcggcaggcg ggcagggaag
     1141 atgaccgatg cattgctcgt cggccagatc ggtctgacga tcgtgctgct tgtcggagcg
     1201 ggcttgatga tccggagctt cctgatcacg cagcagttcg acatcggcgt gaacacggag
     1261 aacctcgtga ccgtgcaggt ccagccgccg aacgcccgtt atccgcagcc ggccgatcga
     1321 ctcgcgttcc aagagcgtct tgcggagcgg ctcgaggcgc tgcccggcgt cgacagcctg
     1381 acgatcgtct cgcagccgcc ggccggcggc gcgcagatgc gcaccctgaa gatcgagggg
     1441 cgggaaatga cggacgcgaa caaccgcctg ccgcttgtcg ggcgcctcgt catcggcccc
     1501 gactatttcg actcgctcga gctgacgatc cgccgcggtc gcggcttcac ggacaacgac
     1561 ggcgcttccg gcgccgaagc cgtcatcgtc aacgagacgt tcgtgacgcg ctattttccg
     1621 gacacggacc ccatcggcag tcggctacgt ctcggcgggg acctcgaccg tggcacggag
     1681 gacgttacgg cgccgtggct cacgatcgtc ggggtgagcc cgccggtctt tcagcagagc
     1741 ccgaaccaag atctgagcgt ccagccgacg gcctacgtgc cgtttcgcca ggagccgccg
     1801 gtcgctttca cggtcctcgc acgcagccgg gtcgccggcg actccgtcgt cacggcgatc
     1861 cgcaacgagc tgcggcagat cgatcctgat ctgccgctct acaacatccg cacgctggac
     1921 gagattcttg cgcagcgaaa ctggccgtat cggatcttcg gcacactgtt cggcacgttc
     1981 gccgtgatcg cgctgctgat ctcgtcggtt ggcatctacg ccgcgacggc gcacggcatc
     2041 ggccaacgaa ggcaagagct cggcgtgcgc atggccctcg gcgcagggcg gcgcgacatc
     2101 ctgtggctcg ttctgcggca gggcatcgcg cgcatcgcca tcggcgtcgt gctaggcgtg
     2161 ctcgcggccg ttggcgtcag ccgcgtgctc gtctcggtgc tcgtgaacac cacggcgacg
     2221 gacccgacga cgttcgtcgc gatctgtctg ctgctcgccg ccgtgacgct gctcgcgtgc
     2281 ttccttccgg ccaggcgcgc cacacggctc gatcccgtcg atgcgcttcg gaccgagtaa
     2341 cgtgacgcgt ctcgctatga gcctcgcggc actcgcggct tgcggcagtg ccgcgggcgg
     2401 cgtgctgcac gagcaggccg agcgcgcggc gcagcgcaac ttcggcgagt atctcgacgc
     2461 gctgcgtatt ccgaacgtcg cggacaagcc ggccgacata gcgcgcaacg ccgcgttctt
     2521 ctcgcgcgcg ttcgagcggc gcggcttcga cgttaggctc gtgacgaacg ccgccggccg
     2581 gcccgtcgtg cttgccgagc ttcctgcgcg ggccgggttg ccgacggtgc tgctctacat
     2641 ccactacgac ggccagccca tagtgcccgc ggagtgggcg caagccgatc cgttcatgcc
     2701 ggtcgtgcgg cgccgcaacg cggcgggcgc gtggcaagac gtcgaaacga acgcgctgca
     2761 agcgcagccg ctcgatccgg agctccgcgt gttcgcacgc tcggcggccg acgacaaagc
     2821 gccgatcatg atgttgctga cggcgttcga cgtgctggcc acgcaagcgg aagcgcccgc
     2881 gtacaacgtc aaagtcatgc tcgacggcga agaggagatg ggctccccga gcctcgcggc
     2941 taccgtcgcg tcggagcgtg cgcggttcgc ggccgacgcg ctcgtgattc tcgacggacc
     3001 gctgcacgcg tctgggcggc cgaccgtcgt gttcggcaat cgcggcatcg cgcaagccac
     3061 gctaaccgtg ttcgggccgc gcgcgccgct gcacagcggc cactacggca actacgcacc
     3121 gaacccggcg ctgcggctcg ccgctctgct cgcctcgatg aaggacgacg acgggcgtgt
     3181 gttgatcccg ggctactacg acggcgttcg cctgaccgaa gcggatcgca tcacgctcgg
     3241 cgacgtcggc gacgacgagg cggcgctgcg cgcgcgcatc ggcatcgcgc gcgccgaaag
     3301 cgtcggcgcg aactatcagg aatcgctgca gtatccatcg ttgaacgttc gcggcatcgc
     3361 ggccggcggc gtcggcgccg tggcgacgaa ctccgtgccg agccaggccg tcgcggagct
     3421 cgatatgcgc acgacgcccg agaccgacgg acgccgtctt tacgagctcg tgcgcgcgca
     3481 catcgagcga gccggttatc acttgatcga cgccgagccg accgacgccg agcgcgcgcg
     3541 gttcgacaag ctcgcccgct ttacgctcgg ctcgacgcag gccgcgatgc ggatgccgtt
     3601 ggactccacg gtcggccgct gggcaaacat ggcgttgcgc gcgccgtcgg cgccgcagcc
     3661 cggcgaggcg cccgtgcaaa tccgcatgat gggcggcacg gtgccgaccg acgtgctgat
     3721 cgccgcgttg cagctgccgt tcgtgctgtt gccgctcgtc aacgacgaca acaaccagca
     3781 cgcgccgaac gagaatctgc gaatcggcaa tttcgtgacg ggcaccgagg cgctctacag
     3841 cctgctggtc acgccgtacc cgcgctgacg acgaggagag cgatacgctg gcgcgcatgg
     3901 agcggctgac ccatagcggc cgcgcgtatc aaggggatac gtcgagcacc cgcctcccga
     3961 gagaaaactt ttgacacctc ggcacgcgct catcgactca aggccacggc gaggccggtt
     4021 aaagtagcgg tccggaacca gggaagatct atgagaacca tccagtcgtt ggcgctcgcc
     4081 gccagttttg tcgccgccgc tcccgtcttc gcccagaaga tgccggacat cggcttcaag
     4141 agcgtcggcc gcggtgcgcc gcttgccgcc agcgtctacg actacaagca agaagtcggc
     4201 ccgaactgga ttcgcacgca gggtcaacag gccggccccg acgtgaaaaa gccgttcccg
     4261 ctgaacggct acctcccgag cgagctgccg aaggactaca agccgctgcc gcgcgacatc
     4321 tacacgagcg acgacttcta taaagacaag gagctctgga gcgacccgcg ctacttccgc
     4381 tgcaacagtc cgcaagcgac cgagtatcag cgcggcgtgc tgcagcgccc cggcgtgaac
     4441 acgtccgaca agatcgagga cgcaccgtgg gggcactgcg acatcagcct gccgcgcgag
     4501 gccatcgtga gcccgtacgg gttcaagacc gcgcacgagc actacgaagc gctgaaggcc
     4561 gagacgacga agcgcggcgg cccgaacgtc tacacgtacg aaaacttccc gaacgccgag
     4621 tggaacggcg tctatcagcg tcccggcggc gcgaacggtg ccggtgccgc caaccagaac
     4681 aactggtact ggggtaggca cacgcagatc ccgacggtcc tgtccgtgct gacgccgaag
     4741 taccaggagt acatggtcca ggaggcctat caccaggtgc gcggcaacgc gctctggccg
     4801 tcgacgttct gttggcccga aggcttcatg cggcgctggt acccggccgc cgtgtcggag
     4861 cactacatca tcgcgacgcc ggatctcgtg cacgtgcgca ccggtgtcgc gcgcaacttc
     4921 attcaagaca cgtacatcgg ccgcgaattc aacatggaag acgtgccgaa gggcggcgtg
     4981 ccgcgtctcg gtcccgcggt gccgcggtgg tacggcgata cgattggctt ctgggacaag
     5041 gacacgctga tcacctggac gagcaacatc cagggctgga agtcgcactc ggagttcgag
     5101 ttttcgagcc agatgcagtc gatcgagatc tacacgccga ttcgcgaagg tggcaagttc
     5161 atcggcctga accacgagac gatcctctac gactccgagg cgttggccac gccggtgcgc
     5221 atcgtgcgca acctgcacaa gatcaacaat ttcaccgaca ccaacgaggt gccgtacgcg
     5281 ttcatcgagt gcgtgcagac gatcttcaac gtcaacggct cgaacactcc gctcgcgcca
     5341 ggcgcgaagc tcgagtacga gatgcccgac atgtacgggc ggccgtgggc gaagatctgg
     5401 aaagagagct tcgagcagaa catgagtggg cccgcggaca cgtccgagga catcttcaat
     5461 ttcgaggaca acaaataaca gtcgtgcgcc ggcggtcggc cggccaagtg acgacggggg
     5521 actgatggcg acgaacaacg gtttcggatt ggcggcaggc gccgtggcgg ttgcggccgc
     5581 gctctgcacg acggcgctcg cgcagcagag cccgcagatc gacttcaaga gcgtcgggcg
     5641 cggcgcgccc gtcatggtcg acgccgccgc gttgcccgtc gtcggcgcga cttaccaagg
     5701 cggcggcggt cccggcggca acggccagcc ccagacgccg cgcacgttcg tcggctcggc
     5761 ggagccaggc cagtcgccga aaggtatcaa gccgctgccg cgcgacatgt tcacgtcgga
     5821 cgacttctac aaggacaagg acctctggtc c
//
LOCUS       DATLEE010000616         2329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10810416, whole genome shotgun sequence.
ACCESSION   DATLEE010000616 DATLEE010000000
VERSION     DATLEE010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10810416"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /gene="acnA"
                     /locus_tag="VM692_13405"
     CDS             <1..1855
                     /gene="acnA"
                     /locus_tag="VM692_13405"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HUQ53216.1"
                     /translation="LRKRGVVGKFVEFYGDGLDHLPLADRATIGNMSPEFGSTCAVFP
                     IDAETLRYLELSGRAREQIDLVEAYAKAQGLWRSSKVARYSDVLTLDMGAVEPSISGP
                     KRPQDRIALKGAGAKVAELLKTAGRGTKAPVTADKQSFDLEDGAVVIAAITSCTNTSN
                     PAVMVGAGLLARNAAAKGLTRKPWVKTSLAPGSKVVTDYLKACNLLGALDNVGFNLVG
                     YGCTTCIGNSGPLPDEIANALSKSDVVACSVLSGNRNFEGRIHPQVKMNFLASPPLVV
                     AYAIAGNMKMDLYQDPLGHGKDGKPVYLRDIWPSTKEIHDLISKHVTSTQFKSSYADV
                     FQGDKNWQAVATPKGENFTWSDESTYVKHPPYFEGMTLTTSTPQNITGARVLAMLGDS
                     VTTDHISPAGNIAATSPAAKYLESLGVTKKDFNSYGARRGNHEVMVRGTFANIRLKNL
                     LVPGTEGGITVHIPSGQQLSIYDAAMKYQAEKTPLIILAGQEYGAGSSRDWAAKGTAL
                     LGVKAVIAESYERIHRSNLIGMGVVPLQFYAGDTPLKLGLTGKESFDITGLAGLKRTR
                     EVTVTATDVGGHKKTFQVVVRIDTPKEQDYFIHGGILQYVLRQLASGKQAA"
     gene            1869..>2329
                     /locus_tag="VM692_13410"
     CDS             1869..>2329
                     /locus_tag="VM692_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HUQ53217.1"
                     /translation="MKLGLPSWLRRGAQTAEAPEAAPPQPPSDSPLNRPRRRRWPWIA
                     AVGVLVVALAWGWSFYWSREPAALWVVAETPAGERAVVGYSTVHTLIDVTNWLLEKPG
                     GYLTNDVLPPGLWLDNMPNFEFGVLTQCRDLARVLRNYHSRSQSQSIEDDDL"
BASE COUNT          443 a          767 c          761 g          358 t
ORIGIN      
        1 gctgcgcaag cgcggcgtcg tcggtaagtt cgtcgagttc tacggcgacg gcctcgatca
       61 cctgccgctt gccgaccgcg cgacgatcgg caacatgtcg ccggagttcg gcagcacgtg
      121 cgccgtgttc ccgatcgacg ccgagacgct gcgctacctc gagctgtcgg ggcgcgcgcg
      181 cgagcaaatc gacctcgtcg aggcgtacgc gaaggcgcaa gggttgtggc gcagctcgaa
      241 ggtcgcgcgc tacagcgacg tgctgacgct cgacatgggc gccgtcgagc cgagcatctc
      301 gggcccgaag cgcccgcaag accgcatcgc actgaagggc gccggcgcca aggtcgccga
      361 gctgctgaag acggccggca gaggcacgaa agcgccggtc accgccgaca agcagagctt
      421 cgatctcgag gacggcgccg tcgtcatcgc cgcgatcacg agctgcacga acacgtcgaa
      481 cccggccgtc atggtcggcg cgggcttgct cgcgcgcaac gccgcagcca aaggcctcac
      541 gcgcaaaccg tgggtcaaga cgagcctggc gcccggctcg aaggtcgtca ccgattatct
      601 gaaggcctgc aatctgctcg gcgccctcga caatgtcggc ttcaacttgg tcggctacgg
      661 ctgcacgacc tgtatcggta actcgggccc gctacccgac gagatcgcga acgcgctgtc
      721 gaagagcgac gtcgtcgcat gctccgtgct gtcgggcaac cgcaacttcg agggccgcat
      781 ccacccgcag gtgaagatga atttcctcgc gtcgccgccg ctcgtcgttg cgtacgcgat
      841 cgccggcaac atgaaaatgg acctctacca agatccgctt gggcacggca aggacggcaa
      901 gcccgtgtac ctccgcgaca tctggccgtc gacgaaggag attcacgatc tgatctcgaa
      961 gcacgtaacc tcgacgcagt tcaagtcgag ctacgccgac gtgtttcaag gcgacaagaa
     1021 ctggcaggcc gtcgcgacgc cgaaaggcga aaacttcacg tggtccgacg agtcgactta
     1081 cgtcaagcac ccgccctact tcgaaggcat gaccttgacg acgagcacgc cgcagaacat
     1141 caccggcgcg cgcgtgctcg cgatgctcgg cgattccgtg acgacggatc acatctcgcc
     1201 ggccggcaac atcgcggcga cgagccccgc ggcgaagtac ctcgagagcc tcggcgtgac
     1261 gaagaaggac ttcaactcgt acggcgcgcg ccgcggcaat cacgaggtca tggtgcgcgg
     1321 cacgttcgcg aacattcgct tgaagaactt gctcgtgccg ggcacggaag gcggcatcac
     1381 ggtccacatc ccgagcggcc agcagctgtc gatctacgac gccgcgatga agtatcaagc
     1441 cgaaaagacg ccgttgatca tccttgcggg tcaggagtat ggcgcgggct cgtcgcgcga
     1501 ctgggcggcc aagggcactg cgctgctcgg cgtcaaagcc gtgatcgcgg aaagctacga
     1561 gcgcatccat cgctcgaact tgatcggcat gggcgttgtg ccgctgcagt tctacgccgg
     1621 cgacacgccg ctgaagttgg gtctgacggg caaagagtcg ttcgacatca ccggcctggc
     1681 cggcctcaag cgcacgaggg aagtcacggt gacggcgacc gacgtcggcg ggcacaagaa
     1741 aacgttccag gtggtcgtgc gcatcgacac gccgaaagag caagactact tcatccacgg
     1801 cggcatcctg cagtacgtgc tacggcaact cgcgtcgggc aagcaagcgg cctgaagcgc
     1861 tagggcacat gaagctcggc ctgccctcct ggctccgtcg cggcgcgcaa acggccgaag
     1921 cgccggaggc agcgccgccg caaccgccga gcgactcgcc gctgaaccgg ccgcgacgtc
     1981 ggcgctggcc ctggatcgca gcggtcggcg tgcttgtcgt cgcgctcgcc tggggatgga
     2041 gcttctattg gtcgcgtgaa ccggcggcgc tctgggtggt ggccgagacg cccgccggcg
     2101 agcgggccgt cgtcggttat tcgacggtgc atacgctgat cgacgtcacg aattggctgc
     2161 tcgagaaacc cggcggctat ctgaccaacg atgtgctgcc gccggggctc tggctcgaca
     2221 acatgccaaa cttcgagttc ggcgtgttga cgcaatgtcg cgacctcgcg cgcgtgctgc
     2281 gcaactacca cagccgctca cagtcgcagt cgatcgagga cgacgatct
//
LOCUS       DATLEE010000617         5977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_9681534, whole genome shotgun sequence.
ACCESSION   DATLEE010000617 DATLEE010000000
VERSION     DATLEE010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9681534"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VM692_13415"
     CDS             complement(<1..683)
                     /locus_tag="VM692_13415"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HUQ53218.1"
                     /translation="MHVEVTDVGKVRDHNEDALGSDPEIGLWVLADGMGGYNAGEVAS
                     GIAVKTIIELVTKACKTEKRGDIESGTGYMRQTIVLRDAILRANKVINQTAQSQPQCE
                     GMGTTLVASLFYDNRVSIAHVGDSRMYRLRGNRFEQITMDHSLLQELVDRGFYSQEEA
                     QRSTNRNYVTRALGVEANVEVEVQEIDVQKGDYFLMCSDGLPDMVEDEDIHLTISTFS
                     NDVRTVGEQL"
     gene            complement(735..1553)
                     /locus_tag="VM692_13420"
     CDS             complement(735..1553)
                     /locus_tag="VM692_13420"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUQ53219.1"
                     /translation="MPASIGRYQLLEPIGRGAMGNVYLGRDPLINRILALKAIDLNVG
                     YDNAELESTAESFLREATIAGNLSHPNIVTIFDVGQNDGLAYIAMEYVRGHHLSEFAA
                     ADRLLPIDTVLDLVGRAADALDYAHRRNVVHRDIKPANIMYDSLSNNLKITDFGIAKL
                     IDANRTRTGIVLGTPAFMSPEQLEGKNVNGHTDLFALGVSLYQLLTGLLPFRGVSMAN
                     LMFVIANEPHQSVTSVRNDLPKWLDEVVDRALAKAPADRFQSAADMAAALRRAA"
     gene            1584..1724
                     /locus_tag="VM692_13425"
     CDS             1584..1724
                     /locus_tag="VM692_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53220.1"
                     /translation="MIGTHESVRYVAELTVDLRDPVKEVRGFVRSAVRRKTAGKIVDL
                     GK"
     gene            2044..3399
                     /gene="radA"
                     /locus_tag="VM692_13430"
     CDS             2044..3399
                     /gene="radA"
                     /locus_tag="VM692_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HUQ53221.1"
                     /translation="MAKARAIYTCGNCGGQQPKWQGQCPDCGAWNTLTESVAVAKRSE
                     RAQYTGQVEFTRLDRVDVGTVARLTTGLGEFDRVLGGGLVPGGVVLIGGDPGIGKSTL
                     LLRALANLAPNLPTCYVTGEESLEQIGLRAQRLGVGEAPLDMLAETCVEAVLARIAAR
                     DKLGVLAIDSIQTLFSEELQSAPGSVSQVRECAAQLTRFAKSRNVATLLIGHVTKEGT
                     LAGPRVLEHMVDTVLYFESDVGSRFRIVRAVKNRFGAANEMGFFVMSEEGFREVKNPS
                     AIFLARHAAPVAGSVTLVTREGTRPVLVEVQALVDRAHGQSPRRLAQGFDTQRLGLLL
                     AILHRHCGFASGDSDVFANVVGGLHVGETAADLAVALAVVSSLTERPVSQDLVVFGEL
                     GLTGEIRPVPFGDERLAEAQKHGFKRAVVPRANAPRRALGVEVVAVERLSEALAVVHA
                     S"
     gene            complement(3408..4667)
                     /locus_tag="VM692_13435"
     CDS             complement(3408..4667)
                     /locus_tag="VM692_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HUQ53222.1"
                     /translation="MVILIIVLLVLSAFFSGTETALLSLNRYRLRHQARSGHRGARLA
                     EWLLQRPDRLIGLILLGNTAANFTTAALVTVLAQRVGGQGAVLIATFLSTIVVLVFCE
                     VAPKTIAALNPPRIALPAALIYYPLLKITYPLVWFVNLFANGLLRLLGVRADQIASHS
                     LSAEELRTVVAEAGVMVPRRHQRMLLSILDLDAVTVEDIMVPRQEIVGLDLDRTWEQN
                     LAIIQTSAHNRLPVYREYIDNIIGVTRVRDLLPELARGELTQALLLERIREPYFVPEG
                     TPLNKQLLNFQQHRRSSAFVVDEYGDVQGLITTQDIVNELVGELDRDSSAEHLGVTKE
                     SDRSYVVDASANVRQLNRVMNWNLPTDGPKTLNGLIVEQLETIPQSGTGVTVAEYPIE
                     ILDTSEHGIKRVRVFAPGSAPPPKAAA"
     gene            4875..>5977
                     /locus_tag="VM692_13440"
     CDS             4875..>5977
                     /locus_tag="VM692_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011704629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HUQ53223.1"
                     /translation="MFDQLTARLTAAIQNVTGRGRITEDNVRDTMRTIRMALLEADVA
                     LPVAKALVDRVRDRALGEEVARSLNPGQAFVKIVHDELLAVLGGESSPLSPQGHPAVV
                     MLVGLQGAGKTTTAAKLAKHLTTTRGGAVLLVSTDVYRPAARDQLATLASDLGVGFHK
                     SASDSPSEIAREALAAAQRGGQRWLILDTAGRLHVDAEMMDEARELHALVSPSETLFV
                     VDSMAGQDAVNSARAFHEALPLTGVILTKADGDARGGVALSVREVTSLPIKLVGTGEK
                     IDALEPFVPARFASRILGMGDVVGLVEQVQKTVDREEMEKLAGKVKQGKGISLEDYRE
                     QLKQMVNMGGIEQILDKLPGLKPEQLAAAKFDP"
BASE COUNT         1022 a         1877 c         2011 g         1067 t
ORIGIN      
        1 agctgctcgc cgactgtgcg aacgtcgttg ctgaacgtgc tgatcgtcag atggatgtcc
       61 tcgtcctcga ccatgtcggg taggccgtcc gagcacatca agaagtagtc gcccttttga
      121 acgtcgatct cttgaacctc gacctcgacg ttcgcttcga cgccgagcgc gcgcgtcacg
      181 taattgcgat tcgtcgagcg ctgcgcttcc tcttgggaat agaagccgcg gtcgacgagc
      241 tcttgcagca gcgagtgatc catcgtgatc tgctcgaagc gattgccgcg caaccgatac
      301 atacgcgagt cgccgacatg cgcgatggac acgcgattgt cgtagaacaa gctggcgacg
      361 agcgtcgtac ccatgccctc gcattgcggt tgcgattgcg cggtctgatt gatgactttg
      421 ttggcgcgga ggatcgcgtc ccgcagcacg atcgtctggc gcatgtagcc cgttccggac
      481 tcgatgtcgc cgcgcttctc cgtcttgcag gctttcgtca cgagctcgat gatcgtcttg
      541 acggcgatgc cgctggcaac ttcgccggcg ttgtagccac ccatgccatc cgcgaggacc
      601 cataagccga tctcggggtc cgagccgagc gcgtcttcgt tgtgatcgcg caccttgccg
      661 acgtccgtga cctcgacgtg caccagtttc cctttgagac tcatgcgttc tgcggcgcct
      721 ttcggtcgcg cgtctcaagc cgcgcgccgc aaggcggctg ccatgtcggc agcggattgg
      781 aaccggtcgg ccggggcctt ggccaaagca cgatccacga cttcgtcgag ccacttgggc
      841 aagtcgttgc gaaccgaagt gacggactga tgcggttcgt ttgcgatcac gaacatgagg
      901 ttggccatgg agactccgcg gaagggcaag agaccggtca gtagctgata caggctaaca
      961 ccgagcgcga ataagtcagt gtgcccgttc acgtttttcc cttcgagttg ctccggcgac
     1021 atgaaggcag gagtccctag cacgatgccc gtccgggtcc ggttggcgtc gatcaacttg
     1081 gcgattccga agtccgtaat cttcaggttg ttcgagaggg aatcatacat aatattggcc
     1141 ggctttatat ccctgtgaac cacgtttcgc cggtgcgcgt agtcgagcgc atcggccgca
     1201 cgacccacga gatcgagcac ggtgtcgata ggcaacagtc ggtcggcggc cgcgaattcg
     1261 ctgaggtgat ggccccgcac gtactccatc gcgatgtagg cgaggccgtc gttctgcccg
     1321 acgtcgaaga tcgtcacgat gttcggatgg ctcaggtttc ccgctatcgt cgcttcgcgc
     1381 aggaaacttt cggcggtgct ctcgagctcg gcgttgtcat agcccacgtt caagtcgatg
     1441 gccttcagcg caaggatgcg gttgattagc gggtcgcgcc cgagatagac gttgcccatg
     1501 gcgccgcggc cgatcggctc gagcagctga tagcgtccta tcgaagccgg catgcgcgcg
     1561 cgctcgtcgt cctcggcggg cgcgtgatcg gcacgcacga gtcggtgcgc tacgtcgcgg
     1621 aactcaccgt cgacttgcgc gacccggtga aagaggtccg cggcttcgtc aggagcgccg
     1681 tgcgtcgcaa gaccgcaggc aagatcgtag atctcgggaa gtagggctac ggtcgccggc
     1741 aacagccgat acagtgccca agcttctttg agtcgtccgc cgtcgactgc gcgtctcgca
     1801 cgttcgagca agtcgccgtc gctggccggc gtggatgctt gcggcttcgg tgggcgtagg
     1861 gacttgaaga agacgctcgt caagagcgca acggctgcga aaagcttgcg cgccagattg
     1921 cggaaccggt ccatggtttg cgtgcccctc ggccgcgcgg agtatcgcac gcgccaccgg
     1981 cagctccgtg tgatgcgcta ccgctttcgc ggtccgtgac gcagttcagc tatcctccgc
     2041 gccatggcga aggcgcgagc gatctacacc tgcggcaact gcggcggcca gcagccgaaa
     2101 tggcaaggcc agtgccccga ttgcggcgcg tggaacacgt tgactgaatc cgtcgccgtg
     2161 gcgaagcgct ccgagcgcgc gcaatacacg gggcaagtcg agttcacgcg gctcgaccgc
     2221 gtcgacgtcg gtactgtggc gcgcttgacg accgggctcg gcgagttcga ccgagtcctc
     2281 ggcggcggac tcgtgcccgg cggagtcgtg ctgatcggcg gcgacccggg aatcggcaag
     2341 tcgacgctgc tgctgcgcgc gttggccaat ctcgcgccga acctgccgac ctgctacgtc
     2401 accggcgaag agtcgctcga gcagatcggg ctccgagcgc agcgtctcgg cgtcggcgaa
     2461 gcgccgctcg acatgctcgc cgagacgtgc gtcgaagccg tgctcgcgcg catagctgct
     2521 cgcgacaagc tcggcgtgct ggcgatcgat tcgattcaaa cgctgttcag cgaggagctg
     2581 cagtccgcgc cgggctccgt gagccaagtg cgcgaatgcg ccgcgcagct cacgcggttc
     2641 gcgaagtcgc gcaacgtcgc gacgctgctg atcggtcacg tcaccaagga gggcacgctc
     2701 gccggcccgc gcgtgctcga gcacatggtc gacaccgtgc tctatttcga gagcgacgtc
     2761 ggcagccgct tccggatcgt gcgcgccgtc aagaaccggt tcggcgctgc caacgagatg
     2821 ggttttttcg tcatgagcga ggagggcttc cgcgaggtca agaacccgtc cgcgattttc
     2881 ttggcgcggc atgctgcgcc cgttgccggc agcgtcacgc tcgtgacgcg cgaaggaacg
     2941 cggcccgtgc tcgtcgaggt tcaggcgctc gtggatcgcg cgcacggtca gtcgccgcgg
     3001 cggctcgcgc aaggcttcga cacgcaacgt ctggggttat tgctcgcgat cctgcaccgg
     3061 cattgcggct ttgcgtcggg cgacagcgac gtgttcgcga acgtcgttgg cggcctgcac
     3121 gtcggcgaga ctgcggccga tctcgccgtc gcgcttgccg tcgtctcgag cctcacggaa
     3181 cgcccggtat cgcaggacct cgtcgtcttc ggcgagctcg gtttgacggg cgagattcgt
     3241 cccgtcccat tcggcgacga gcggctcgca gaggctcaga agcatggctt caagcgcgcc
     3301 gtcgttccac gcgccaatgc tccgagacgc gcgctcggcg tcgaagtcgt agccgtcgaa
     3361 cggttgagtg aggcgctcgc ggtcgtgcac gcgagctgag cgcgccgcta agcggcggct
     3421 ttgggcggcg gagcggagcc cggcgcgaaa acgcgcacgc gtttgatgcc gtgctcgctc
     3481 gtgtcgagga tctcgatcgg atactcggcg accgtgacgc cggtccccga ctggggaatg
     3541 gtctcgagct gctcgacgat gagcccgttc agcgttttcg ggccgtccgt cggtagattc
     3601 caattcatga cgcgattcag ctgacgcacg ttcgcgctcg cgtcgacgac gtagctgcgg
     3661 tcgctctcct tggtgacgcc gaggtgctcg gcgctcgagt cgcgatcgag ctcgccgacg
     3721 agctcgttga cgatgtcctg ggtcgtgatc agcccctgca cgtcgccgta ctcgtcgacg
     3781 acgaaggccg agctgcggcg atgctgctga aagttcagga gctgcttgtt gagcggcgtg
     3841 ccttcgggga cgaagtacgg ctcgcggatt cgctcgagca gcagcgcttg cgtcagctcg
     3901 ccgcgcgcga gctcgggcaa tagatcgcgg acgcgcgtga cgccgatgat gttgtcgatg
     3961 tactcgcggt acacgggcaa gcggttgtgc gcgctcgtct ggatgatcgc taggttctgt
     4021 tcccaggtcc gatcgagatc gagccccacg atctcctggc gcggcaccat gatgtcttcg
     4081 accgtcacgg cgtcgagatc gaggatgctg agcagcatgc gctggtggcg ccgcggcacc
     4141 atgacgccgg cctcggccac gaccgtgcgc agctcctcgg cgctcaacga gtgcgatgcg
     4201 atctgatcgg cgcgcacgcc gagcaaccgc agtaagccgt tggcgaacag gtttacgaac
     4261 cagacgagcg ggtaggtaat tttcagcagc gggtagtaga tcaaggctgc cggcagcgcg
     4321 atccgcggcg gattcaatgc cgcgatcgtc ttcggtgcca cctcgcagaa gacgaggacg
     4381 acgatcgtcg acaaaaacgt ggcaatcaag accgcccctt ggccgccgac ccgctgggcc
     4441 aaaacggtga caagggccgc cgtggtgaag ttcgcggcgg tattgccgag caagatgagg
     4501 ccgatgagcc gatcggggcg ttgcagcagc cattcggcaa gtcgcgcgcc gcggtggccc
     4561 gagcgggcct gatgccgtag ccgatagcga ttgaggctca gcagcgccgt ctccgtgccc
     4621 gagaaaaacg cggaaagcac gagcagcacg atgatcaaaa tgaccaacaa gccaacggga
     4681 gcctggtcac tcaaagcttg tggagcctcc gaacgggctg acgccgaatt tttagagtgg
     4741 aggaatgcgg aatagggtgt caaggaaggt gcggcggggg ctgcaccagc gctcattggg
     4801 gcacggcggg gtgtgaccga ttgcgcatgc taggctgtcg ccctccctga ccgagactat
     4861 cccaagagtt acgcatgttc gatcaactga cagctcgcct gaccgcggcc atccagaatg
     4921 tgaccggtcg cggccgcatt accgaggaca acgttcgcga caccatgcgc acgattcgca
     4981 tggcgttact agaggccgac gtcgcgttgc ccgtggcgaa ggccctcgtc gaccgggtcc
     5041 gcgaccgagc gctcggcgaa gaagtggcgc gcagcctcaa tcccggccaa gcgttcgtca
     5101 agattgtcca cgacgagctc ctcgcagtgc tcggcggcga gtcgagcccg ctgagcccgc
     5161 agggccatcc ggccgtcgtg atgctcgtgg gactgcaagg cgccggcaag acgacgacgg
     5221 ccgcgaagct cgcgaagcac ttgacgacta cgcgcggggg cgccgtgctg ctcgtctcca
     5281 ccgacgtcta tcggcccgcg gcgcgcgatc agctcgcgac gttggcgtcc gacttgggcg
     5341 tcggcttcca caagagcgcg agcgactcgc cgtccgagat cgcgcgcgag gcgctcgcgg
     5401 cggcgcaacg cggcggccag cgctggctga ttctcgatac ggcgggccgc ctgcacgtcg
     5461 acgccgagat gatggacgag gcacgcgagc tgcacgccct cgtgagcccg agcgagacgc
     5521 tattcgtcgt cgactcgatg gcgggccagg acgccgtgaa ttccgcacgt gccttccacg
     5581 aggcgctgcc cctgacgggc gtcatcctga cgaaggccga cggcgatgcc cgcggcggcg
     5641 tcgctctttc ggtgcgcgag gtgacgagcc tgccgatcaa gctcgtcggc accggcgaga
     5701 agatcgatgc gctcgagccg ttcgtaccgg cgcgcttcgc gtcgcgcatc ttgggcatgg
     5761 gcgacgtcgt gggcctcgtc gagcaggttc agaagaccgt cgaccgcgag gaaatggaaa
     5821 agctcgccgg caaggtcaag caggggaagg ggatcagcct cgaggactat cgcgagcagc
     5881 tcaagcagat ggtcaacatg ggcggcatcg agcaaattct cgacaagctg cccggcctca
     5941 agcccgagca gctcgcggcg gcgaagttcg atccgaa
//
LOCUS       DATLEE010000618         4429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2977148, whole genome shotgun sequence.
ACCESSION   DATLEE010000618 DATLEE010000000
VERSION     DATLEE010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2977148"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VM692_13445"
     CDS             complement(<1..676)
                     /locus_tag="VM692_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53224.1"
                     /translation="MRAQLAALAALVVAHSALAQQAPRASTTPAGSGGSSNAALINAA
                     PEGPPPKLPDGTPDLSGVWVGSGPIGDISMGLVPGEELVLLPAAKTLMESRQAADDPE
                     ARCLPTGVPRIAPYPWRIVQSPAFGKATHLFFLYEGNIHSFRLIFMDGREHPDDLEES
                     WYGHSVGHWEGDTLVIETVGFNDLFWFDFVGHPHTTQLRTVERYTRTKMNELEVVTTV
                     YDAGAYA"
     gene            complement(680..1072)
                     /locus_tag="VM692_13450"
     CDS             complement(680..1072)
                     /locus_tag="VM692_13450"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53225.1"
                     /translation="MKVVCSVLAAFGVALSAMPVDAHHSFAAQYDASKPVTLKGTVTK
                     VEWTNPHARFYINVPGADGKVVNWNLELASPNYLKRAGWSSGSLKEGDEVTVEGAMAR
                     SGANMANASTVTFPDGTRVFARSAADNR"
     gene            1172..1645
                     /locus_tag="VM692_13455"
     CDS             1172..1645
                     /locus_tag="VM692_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ53226.1"
                     /translation="MRVLPLFVAAVAFEAAAVAQLPVEAHRRQQELLPSADVQLAANK
                     KLVFDFWREVLQARHVDRAPQYLSESYIQHNPNVPTGRAAFVEFFGRSAPEPIKDTID
                     DLVRIVAEGDVVVLAFRRELPDLAREGQTYTTTWFDMFRIADGKIVEHWDYGTKE"
     gene            complement(1671..2498)
                     /locus_tag="VM692_13460"
     CDS             complement(1671..2498)
                     /locus_tag="VM692_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007625325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HUQ53227.1"
                     /translation="MASPCEVHVAAGDRALAERILAVAAGEAARIEAKFSRYRRGNVI
                     DAINTAQGRPVVVDDETARLLDYAAQLFELSDGKFDVTSGVLRRAWRFDGSDRLPSRE
                     SVAALLPIVGWHKVRWRAPELTLPAGMELDLGGIGKEYAVDRAAALVRPLSNRCLVNF
                     GGDLLALGPQADGQPWRVGIESLADSAVAARHIELAVGALATSGDARRYLLQDGVRYG
                     HILDPTTGWPVLGAPRSITVAADSCTQAGMLATFALLQGAEAEAFLTTQGVQFWALR"
     gene            complement(2578..3894)
                     /locus_tag="VM692_13465"
     CDS             complement(2578..3894)
                     /locus_tag="VM692_13465"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HUQ53228.1"
                     /translation="MQLKGRRALRDIGTRLAAATCALLGPAALPGKVSAQELMPWDID
                     TSLLIYSESDSRVRDTSLNLRARKELREEKFLNLTFALDSLTGASPSGAVPASTVQTF
                     TNPSGNARYTVQPREQALDTSFLDTRTAITASWEMPVTRLALLSVGASLSDEYDYTHT
                     GIDARLARDFNNRNTTLSFGLALANDTIDPVGGSPVPLAPMLGLGNQANKRGDQSKDV
                     TDFLLGVSQVLNRHTIIQVNYSLSQSDGYLTDPYKILSVVDPVTGNPVAGPAGSGLNR
                     YLFESRPDTRDKQSVYALLKRDFDGDVLEASYRYMTDDWGVDSHTLELRYRFGFGESK
                     YLQPHVRFYQQTAADFYRTVLFSNAALPQFATADHRVGEFDGLTVGLKYGQATPHGGE
                     WSARIEYYTQTGDPSPGSAVGALAGFDLYPDLNALIAQFSYNFGGR"
     gene            complement(3885..4103)
                     /locus_tag="VM692_13470"
     CDS             complement(3885..4103)
                     /locus_tag="VM692_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009836399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HUQ53229.1"
                     /translation="MKLASRLALAASATTALAACAGVEPWDRDVLARPDMQIVSDPIE
                     AATDDHIYFSKEASSGGQGFGGGGCGCN"
     gene            complement(4135..>4429)
                     /locus_tag="VM692_13475"
     CDS             complement(4135..>4429)
                     /locus_tag="VM692_13475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HUQ53230.1"
                     /translation="NSMQEKYGPQGLQIVAVNLDKERALADAFLKEVPATFELRFDPA
                     GELAKQFKVQTMPSSFLLDPDGNVLAEHFGFRTADSAEYENGIKDALAKRPAR"
BASE COUNT          729 a         1491 c         1448 g          761 t
ORIGIN      
        1 tcgcgtacgc gccggcgtcg tagacggtcg tcacgacctc gagctcgttc atcttcgtgc
       61 gcgtgtagcg ctcgaccgtg cgcagctgcg tcgtgtgcgg gtggcccacg aagtcgaacc
      121 agaacagatc gttgaagccg accgtctcga tgacgagcgt gtcgccttcc cagtgcccga
      181 ccgagtggcc gtaccagctt tcctcgagat cgtcgggatg ctcgcggccg tccatgaaga
      241 tcagacggaa gctgtggatg ttgccttcat acaagaagaa caaatgagtc gccttgccga
      301 aggcgggact ctgcacgatg cgccacggat agggcgcgat gcgcggcacg ccggtcggca
      361 agcagcgcgc ttcgggatcg tcggccgctt gccgcgactc catcagcgtc ttcgcggcgg
      421 gcagcagcac gagctcctcg ccgggcacga ggcccatgct gatgtcgccg atcgggcccg
      481 agccgaccca cacgcccgag aggtccggcg tgccgtcggg cagcttcggc ggcgggcctt
      541 cgggcgccgc gttgatcagc gccgcgttgc tcgagccgcc gctgccggca ggcgtcgtgg
      601 aagctctcgg cgcttgttgc gcgagcgccg agtgcgcgac gacgagcgcg gccaatgccg
      661 ccagttgagc gcgcatgagc tagcggttgt ccgctgctga acgcgcgaag actcgagtgc
      721 cgtcgggaaa cgtgacggtg ctcgcgttgg ccatgttggc gccgctacgc gccatcgcgc
      781 cttcgacggt cacctcgtcg ccctctttca gcgacccgct cgaccagccc gcacgcttca
      841 aatagttcgg gctcgcgagc tcgaggttcc aattgacgac cttgccgtcg gcgcccggca
      901 cattgatata gaaacgcgcg tgtgggttcg tccactcgac tttcgtgacc gtgcctttca
      961 gcgtcacggg cttgctggcg tcgtactgcg ccgcgaacga gtgatgtgcg tcgaccggca
     1021 tggcggacaa cgccacgccg aacgccgcga gaacgctgca aacgactttc atcgctgtct
     1081 ccccctcttc gatgctcaaa ctctagccaa gacgccgcca cggtgtcgag tgttatagtc
     1141 gattgagctg cttaagattt ccgagagcac tatgcgcgtg ttgccgttgt tcgtcgccgc
     1201 cgtcgccttc gaggccgcgg ccgtcgcgca gctacccgtc gaggctcacc gccgccagca
     1261 agagctgctg ccgagcgcag acgtgcagct cgcagccaac aagaagctcg tgttcgactt
     1321 ttggcgcgag gtgctgcaag cgcggcacgt cgaccgcgcg cctcaatacc tctccgagag
     1381 ctacatccag cacaacccca atgtgccgac agggcgagcc gcgttcgtgg aattcttcgg
     1441 gcgcagcgcg ccggagccca tcaaagacac gatcgacgat ctcgtccgca tcgtcgccga
     1501 gggcgacgtc gtcgtgctgg ccttccgccg cgagctgccg gacctcgcgc gtgagggcca
     1561 aacttacacg acgacgtggt tcgacatgtt ccgcatcgct gacggcaaga tcgtcgagca
     1621 ttgggactac ggcacgaagg aatagcgccg agcccgcgct cagcgcagcg tcagcgcaac
     1681 gcccagaact gaacgccttg cgtggtcaga aacgcttcgg cttctgctcc ctgtaacaac
     1741 gcgaacgtcg ccagcatgcc ggcttgcgtg cagctatcgg cggccaccgt gatcgagcgc
     1801 ggcgcgccga ggacgggcca gcccgtcgtc gggtcgagaa tgtggccgta gcgcacgccg
     1861 tcttgcagca gataccgccg tgcgtcgccg ctcgtcgcaa gcgcgccgac cgcgagctcg
     1921 atgtgccgcg ccgcgaccgc cgagtcggcg agcgattcga tgccgacgcg ccacggttgg
     1981 ccgtcggctt gcgggccgag cgcgagcaag tcgccgccga aattcacgag acaccggttc
     2041 gacaggggcc gcacgagtgc agccgcgcga tcgacggcgt actccttgcc gatcccgccg
     2101 agatcgagct ccatacccgc gggcagcgtg agctccggcg cgcgccaacg aaccttgtgc
     2161 caaccgacga tcggcaacag cgccgcgacc gactcgcggc tcggcaaccg atcgctgccg
     2221 tcgaagcgcc acgcacgccg cagcacgccc gacgtgacgt cgaacttgcc gtcgctgagc
     2281 tcgaagagtt gcgcggcgta gtcgagcagc cgcgccgtct cgtcgtcgac gacgacgggc
     2341 cgcccctgtg ccgtgttgat cgcgtcaatg acgttgccgc gccggtaacg gctgaacttc
     2401 gcctcgatgc gggcggcctc gcccgccgct acggccagaa tccgttcggc gagcgcacgg
     2461 tcaccggcgg cgacgtgcac ctcgcatggg ctcgccatcg cttggaagac gccgagccac
     2521 cccgtgcccc gcggcgaaag cgacgctgcc gccgcgggtg ccggcgctgc gggggtgtca
     2581 gcgcccgccg aagttgtagc tgaactgggc aatcaacgcg ttcaagtcgg gatagagatc
     2641 gaaaccggcc aacgcgccga cggccgagcc cggcgacgga tcgcccgtct gcgtgtagta
     2701 ctcgatgcgc gccgaccact cgccgccgtg cggcgtcgcc tggccgtact tcaatccgac
     2761 ggtgaggccg tcgaactcgc cgacgcggtg gtccgccgtc gcgaactgcg gcagcgccgc
     2821 gttcgagaac agtacggtgc gataaaaatc cgcggccgtc tgttggtaga agcggacgtg
     2881 cggctgcaga tacttgctct ccccgaagcc gaagcgatac cgcagctcga gcgtgtgcga
     2941 gtcgacgccc cagtcgtcgg tcatgtagcg gtacgacgcc tcgagcacgt cgccgtcgaa
     3001 atcgcgcttc agcaacgcgt agacgctctg cttgtcgcgc gtatcgggcc ggctctcgaa
     3061 caggtagcga ttgagcccac tcccagccgg gcctgcgacg gggttgcccg taacggggtc
     3121 gacaacggac agaatcttgt agggatcggt cagataaccg tcggactgac tcaacgaata
     3181 attgacttgg atgatcgtat ggcgattcag cacctgcgaa acgccgagca gaaaatccgt
     3241 cacgtccttc gactggtcgc cgcgcttgtt cgcctggttc ccgagcccca gcatcggcgc
     3301 cagcggcacg ggactgccgc cgacggggtc gatcgtgtcg ttggcgagcg ccaagccgaa
     3361 cgacaacgtc gtgttgcgat tgttgaagtc gcgcgcgagc cgcgcgtcga ttccggtatg
     3421 cgtgtagtcg tactcgtccg acaggcttgc gccgacggac agcagcgcca ggcgagtcac
     3481 gggcatttcc cagctcgccg tgatcgccgt gcgcgtgtcc aagaacgacg tgtcgagcgc
     3541 ctgctcgcgc ggctgcacgg tgtagcgcgc gttgccggac gggttcgtga aggtctgtac
     3601 ggtgctcgca ggcaccgcgc cgctcggcga ggcgcccgtc agcgagtcga gcgcgaacgt
     3661 caggttcaag aacttttcct cgcgcagctc cttgcgcgcg cgcaagttca agctcgtgtc
     3721 gcgaactcgg ctgtcggact cgctatagat cagcagcgac gtatcgatgt cccacggcat
     3781 gagctcttgc gcgctgacct tgccgggcag agcggccggc ccgagcaatg cgcaagtcgc
     3841 cgcagcgagc cgggtgccga tatcgcgcag cgcgcggcga cctttcagtt gcatccgcag
     3901 ccgcctccgc cgaatccttg cccgccgctc gaggcttcct tgctgaagta gatgtgatcg
     3961 tcggtggcgg cctcgatcgg gtcgctgacg atctgcatgt cggggcgcgc caagacgtcg
     4021 cggtcccacg gctcaactcc tgcgcacgcc gcgagcgcgg ttgtcgcgct cgccgcaagt
     4081 gccagcctcg aagctagctt catcgcggtc acagtcattc tcctttactt gtcgttagcg
     4141 ggcggggcgc ttcgccagcg cgtctttgat tccgttctcg tactcggcgg aatcggccgt
     4201 tctgaagccg aaatgctccg cgagcacgtt gccgtccgga tcgagcagga acgagctcgg
     4261 catcgtttgc accttgaatt gcttcgcgag ctcgccggcg gggtcgaagc gcagctcgaa
     4321 cgtggccggg acctccttca agaaggcgtc ggccaacgcg cgctccttgt cgaggttgac
     4381 cgcgacgatc tgcaagccct gcgggccgta tttttcctgc atcgaattg
//
LOCUS       DATLEE010000619         3102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_4444197, whole genome shotgun sequence.
ACCESSION   DATLEE010000619 DATLEE010000000
VERSION     DATLEE010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4444197"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /gene="prmA"
                     /locus_tag="VM692_13480"
     CDS             <1..615
                     /gene="prmA"
                     /locus_tag="VM692_13480"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HUQ53231.1"
                     /translation="AWQPKLEQAVKARPIGARLWLAPPDDEHVPADRSIVRINMGLAF
                     GTGEHPTTALCLDWLERHVAAGSTLLDYGCGSGILALAALALGASNAIAVDNDGQALT
                     ATHANAELNGVGERLLVAAPEAMPAVSVDALVANILAGPLVALASTFARLVRPGGWLI
                     LSGILERQASEVAAAYAPYVGALEQTARGGWVLLTGRRDSIMRA"
     gene            653..1759
                     /locus_tag="VM692_13485"
     CDS             653..1759
                     /locus_tag="VM692_13485"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HUQ53232.1"
                     /translation="MLFTRCPECDTTFRVTDETLKKANGQVRCGRCASVFNAYAELQD
                     PTAKNFEAEAPKRERLTEPSVAATVAATPTAIPPTAMPAPAVPPTNEPPTPISEAAPA
                     AAPVSAAPPSTLTSSDAAASSIGAESVAGVVAEANLASADQEQEAAFGAAATAFDADL
                     SATDVERVLTSNAGPPVPQSAYVWPATAGTAERRPASRWWSIAAALALLALGAQALHY
                     FRAELASNPTFGPLLARAYAALGAEIAPSWDVHQYQIIDWVATAEPNSRGVGSLKITA
                     RIQNRGPQRQPYPAVQLRLKDRYEDAVGSRMFTPAEYLPRDTPRGRLMSPGETARAEI
                     EVVDPGPDAYGFELDVCIEVEASLVTCGTDEVFL"
     gene            1984..2304
                     /locus_tag="VM692_13490"
     CDS             1984..2304
                     /locus_tag="VM692_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007022200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUQ53233.1"
                     /translation="MTAMTSTKRTSRKKTKRVPAHHAVHVGSRNKLLSNLTDECLNSY
                     FESLNGHKPGDLYQLVIGEVEKPLFRAVLTYTNGNQSEAAEILGINRGTLRKKLKDYK
                     LQGR"
     gene            2376..>3102
                     /gene="purH"
                     /locus_tag="VM692_13495"
     CDS             2376..>3102
                     /gene="purH"
                     /locus_tag="VM692_13495"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006915364.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HUQ53234.1"
                     /translation="MRRRALLSVADKQGLTPFAAALESLGFEILSTGGTAKALRAAGV
                     AVTDVAAVTGFPEIMDGRVKTLHPHIHGGLLARRGVDDAVLETHGIGVIDLLVVNLYP
                     FEATTARADCTDAEAIENIDVGGPAMLRAAAKNHEHIAVVVDHGDYEAVLAALRDGGV
                     PAAMRRELAIKAFSHTARYDLGISHYLRTHSASPDDWPSPLLKSWELLQPLRYGENPH
                     QRAALYRTAERVPGSVAHAQQLQG"
BASE COUNT          576 a         1044 c         1006 g          476 t
ORIGIN      
        1 gcgtggcagc cgaagctcga gcaagccgtg aaggcacgtc ccatcggcgc ccggctttgg
       61 ctggcgcctc cagacgacga gcacgtgccc gcggaccgca gcattgtgcg catcaacatg
      121 gggctcgcgt ttggcaccgg cgagcatccc acgacggcgc tgtgcctcga ctggctggag
      181 cgccacgtcg ccgcgggctc cacgctgctc gactacggct gcgggtcggg gattctcgca
      241 ctggccgcgc tcgccctcgg cgcgtcgaac gcgatcgccg tcgataacga cggtcaagcg
      301 ctgacggcga cgcacgccaa tgccgagctc aacggtgtcg gcgagcggct gctggtcgca
      361 gcgcccgaag caatgccggc cgtcagcgtc gacgcgctcg tggccaacat cctcgcaggc
      421 ccgctcgttg cgctcgcgtc tacgttcgcg aggctcgtac ggcccggcgg ctggctgatc
      481 ttgagcggca tcctcgagcg gcaagcgtcc gaggtcgcgg ctgcctatgc accgtacgtc
      541 ggcgcgctcg agcaaaccgc aagaggcgga tgggtgctgc tcacgggccg ccgcgattcg
      601 atcatgcgag cttagacccg ctagaatcgc cgcgtcacga gtcaggcgag ccatgctgtt
      661 cacgcgctgc cccgaatgcg acacgacctt ccgcgtcacc gatgagacgc tgaagaaggc
      721 caacggtcag gtccgatgcg gtcgttgtgc cagcgtgttc aacgcgtacg ccgagctgca
      781 ggatccgacg gcgaagaact tcgaggccga agcaccgaag cgagagcgac tgaccgagcc
      841 tagcgtcgcc gcgacagtcg ccgctacgcc gactgcgatt ccgccgactg cgatgccggc
      901 gcctgcggtc ccgccaacga atgagccgcc gacgcccata tcggaggccg cgccggccgc
      961 cgcccccgtg tcggcggcgc cgccgagcac gctgacatca agcgatgcgg cggcgtcgag
     1021 catcggcgcc gaatcggtcg ctggcgtcgt ggctgaagcg aatctcgcgt ctgccgacca
     1081 agagcaggaa gcggccttcg gcgcggccgc cacggcgttc gacgccgact tgagcgcgac
     1141 ggacgtcgag cgcgtgttga cgtcgaatgc cggaccaccc gttccgcagt cggcctacgt
     1201 gtggcccgcg acggccggca cggccgagcg gcgtcctgca agccgctggt ggtccattgc
     1261 ggcggcgttg gcgctgctcg cgctcggcgc gcaagcgctt cactattttc gcgcggagct
     1321 cgcgagcaac ccgacgttcg gtccattgct cgcgcgagcg tacgcggcgc tcggtgcaga
     1381 gatcgcgccg agctgggacg tgcatcagta tcaaatcatc gattgggtcg cgacggccga
     1441 gccgaactca cgcggcgtcg gtagcttgaa gatcacagcg cgcattcaga atcgcgggcc
     1501 gcaacgtcag ccatatcccg ccgtgcagct gcgtctcaaa gatcgctacg aagacgccgt
     1561 cggcagtcgc atgttcacac ccgcggagta tctgccgcgc gacacgccgc gcgggcgact
     1621 catgtcgccc ggtgaaacag cgcgagccga aatcgaagtc gtcgatccag gccccgatgc
     1681 gtacggattc gagctcgatg tttgcatcga agtcgaagca tctttagtaa cctgcggtac
     1741 cgacgaagta tttctttgac acaaaaattt cgcgcgcgcg tgaatcaaaa ttcgcgcgct
     1801 gcgtgctatg ctgcccgcac cctgggtcgg tacttctact gcaagaaacg gggttagcgt
     1861 caaacgaacg gatacctggc cacagccggg acctggccgc tttttcgcac tcgcggccgc
     1921 acgcgcgacg ctacagagga gtaccgagcg ggcactcgca acgttctagg gggagagaac
     1981 gtgatgacgg ctatgacgag cacgaaaaga acatcgcgca aaaaaacgaa acgtgtccct
     2041 gctcaccatg cggtgcacgt cggctcgcgc aacaagttgc tgagcaactt gaccgacgaa
     2101 tgcctgaaca gttatttcga aagcttgaac ggccataaac ccggcgatct ctaccagctc
     2161 gtcatcggcg aagtcgaaaa gccgttgttc cgtgccgtgc tgacgtatac caacggcaac
     2221 caaagcgagg ccgccgagat tctcggcatc aatcgcggca cgctgcgcaa gaagctcaaa
     2281 gactacaagc tccagggtcg atgagcccga cccgcaagac acgacgaccg cgcacagaac
     2341 gtccgtcggg ttcgagcgag ccgaaacacg gccctttgcg gcgccgagcc ctgcttagcg
     2401 ttgcagacaa gcaaggtctg acgccgttcg cggcggcgct cgaatcgctc gggttcgaga
     2461 tcctgtcgac gggcggcacg gccaaagccc tgcgcgcggc cggcgtcgcc gtgacagacg
     2521 tcgccgcggt cacgggtttc cccgagatca tggacggacg cgtcaagacg ctgcatccgc
     2581 acatccacgg cggcctgctg gcacgccgcg gcgtcgacga cgccgtgctc gagacccatg
     2641 gaatcggcgt catcgacctg ctagtcgtca acttgtatcc attcgaggcg acgacggcgc
     2701 gcgccgattg caccgacgcc gaggccatcg agaacatcga cgtcggcggg cctgcaatgc
     2761 tgcgcgccgc cgccaagaat cacgagcaca tcgctgtcgt cgtcgaccat ggcgactacg
     2821 aggccgtgct cgccgcactc cgggacggcg gcgtcccagc cgccatgcgg cgggagctcg
     2881 cgatcaaggc attcagccac acggcgcgct acgacttagg catcagtcac tatctgcgca
     2941 cgcatagcgc gtcgccggac gactggccga gcccgctgct gaagagctgg gagctcttgc
     3001 agccgctgcg ctacggcgag aacccgcatc agcgcgccgc gttgtaccgg actgcggagc
     3061 gcgtgccggg ctcggtagcg catgcgcagc agctgcaagg ca
//
LOCUS       DATLEE010000620         6678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2978906, whole genome shotgun sequence.
ACCESSION   DATLEE010000620 DATLEE010000000
VERSION     DATLEE010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2978906"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VM692_13500"
     CDS             <1..817
                     /locus_tag="VM692_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HUQ53235.1"
                     /translation="DRAAVERFFAAERPEHVFMAAAKVGGIVANDAYPADFIRDNLEI
                     QTNVIDAAYRNGTRKLCFLGSSCIYPRLAPQPLEESSLLTGPLEPTNQWYAVAKIAGI
                     KMCQAYARQYGFNAISVMPTNLYGPGDNFDLQTSHVLPALLRKLHAAKQNGDAEVTVW
                     GSGTPRREFLYVDDLADALLFLMERYESPEIINVGCGEDVTIAELARLVAEIVGFRGS
                     LVFDRSKPDGTPRKLLDVSKTRALGWQPKTSLADGIRATYDWYLANGSQARAG"
     gene            complement(828..1730)
                     /locus_tag="VM692_13505"
     CDS             complement(828..1730)
                     /locus_tag="VM692_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53236.1"
                     /translation="MVINDLVISTETGGLGNRLKSWVSSMRLAADARVHWPVTKNMPA
                     AFGELFVNECAIDAVPSGAAVYGSWRLAVLPQDEPHLPAGFATVGAGAHPLVRGLGKA
                     WWSLTGERTDRYRFMVFPKQHSRRSTRADARHIDLEYERIPHYFRGVYGELFRRIAVR
                     PEIAAAVAHWAAANLDDDVVGVQVRTWRDDARRHRKYHLPAVRRLTGLLDGAGAGARF
                     LVVSDSDDIAPMLSERYGAARVLCYPRTTSRADSWLAPRGITEDLIDMLLLARTRRLF
                     ASYLSTFSEVAWWLGGTAARVSVF"
     gene            1905..4316
                     /locus_tag="VM692_13510"
     CDS             1905..4316
                     /locus_tag="VM692_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HUQ53237.1"
                     /translation="MTALSTRHGIAGAALAVCLVFATAPLHGQPDFGDNTSQWANDGE
                     CDDPRFEGAGSADTLLDEDRGHDATDCRTLFTAGRITLRSAAASNNGNVNFGNDSSRW
                     ARDGECDDPRFEGEGSADTLLDEDNGHDAADCRSLFQAGRIALRGAASSVDFGDDSSE
                     WARDGECDDPRFEGDGSAETLLEADAYHDATDCRTLLSQNRISLRGDAAATDLNRGRL
                     EKGDDTLSSGEYMDAYTFTGSTGQRAVVDLRSGDFDPYVFVRAPSGKQFDNDDFEGDA
                     NRSLLALDLTESGEYKVTVTTYKKDETGGYTLSIDVGSSSSIASRIDRNGRLENGDVT
                     LTSGEFVDTYEFEGSPGQHVAIDLRSSAFDTYLILRDPAGEQTENDDATESTDVGHSS
                     IEADLTEAGTYKVLVTSYETGQSGAYSLTIDPAAQPRGAPATRDVTTLTVGRTVSGDL
                     GSSDPTYEAGEYHDTYVFDGDEGETVRIELTSTDFDTYLGLITPSGEEIANDDFEGDS
                     ARSVIALTLSEAGRYRVQATSYGAAETGRYGLELTTSTASIPIERRSQGRVYGLFTGI
                     SDYPGSESDLNYTAEDATRIRDALVRGGGMRAEDAVTLVDGDVTVGNMTAAIRDIGRR
                     MQPQDTFVMFYSGHGGQVRRSAPTSTDPDGLDETLALYDGALLDDDLASLFDEIHNGT
                     VLLWLDSCFSGGFAKDIVSAPGRMGIFSSEEDITSNVASKFRAGGYLSAFLDEAIAQG
                     LADDDKDSSITAIELSQYLHERYRADVKSARPVEVVRTEMTLGYQHLVVDRGSIGAYD
                     VLFER"
     gene            complement(4365..5609)
                     /locus_tag="VM692_13515"
     CDS             complement(4365..5609)
                     /locus_tag="VM692_13515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HUQ53238.1"
                     /translation="MADTVTRATSGAAGRVTPPWYLIFFFGALAALGPLSIDAYLPGI
                     PAMARDFGVSVVSLQNTLNVFLIGYAIGQFFGGALSDQIGRKRIGYSGLAVYVVTSMA
                     IAFSASVGQMLTLRFFQAIGGGFATVICLATVRDIYPVEQLGRRFATVTMVLLVAPLV
                     APALGAAVLPLGWETIFVLKAVYGAVLLGAYSVLVHETRPGRLASLSLRSVFRQCGEV
                     VRRRVDSRYLPIRYAMAMAFSASCMMIFVTNSSFIYMQYFGVTPARFSMLFATSVLGF
                     MSMNLFSMWRLKGHNAGTFFRVGLTVQVLVVTGLLTVIATGHAALITVVPFIVLAVAT
                     LGLVGPAGSSRYMGFFRELAGSASSVYTTMMFLLGGTFGWLSGMLNDGTLLPVAAMML
                     GASLAANAISWHLPRRLELAPH"
     gene            complement(5609..6175)
                     /locus_tag="VM692_13520"
     CDS             complement(5609..6175)
                     /locus_tag="VM692_13520"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HUQ53239.1"
                     /translation="MRQLPPTTVCAWRELERRTVAECRIFNVERSVAASPVDDKPRTF
                     HRIESVDWAQIVPVTADNQVVLVRQYRHGNRRVTLEIPGGLVDPGEDPAVSALRECLE
                     ETGYRARTALPLGVVAPNPALFGNRLHAFYAVDVVPEQAVQNTGTEITEVVLVPVTDL
                     EELLVAGEIDHALVAGTLWRYLRLHVPR"
     gene            complement(6172..>6678)
                     /locus_tag="VM692_13525"
     CDS             complement(6172..>6678)
                     /locus_tag="VM692_13525"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HUQ53240.1"
                     /translation="QARAAAQAEATASLTGDALDARLADIDNVAGANHLEQSWLGRVG
                     HWVEPVFRPLGWDWRVSAGVIAGFPAREVVVAVLGTIYAVGGEAEEETLAGRLRAAQH
                     VDGTPVFTLPMVLGLLLFYAWCLQCAATLAVIRRETNSWRWPLFAWSYMTALGYGGAL
                     LIYQLGTA"
BASE COUNT         1250 a         2270 c         2202 g          956 t
ORIGIN      
        1 cgaccgcgct gctgtcgagc gcttcttcgc cgccgagcgc cccgagcatg tcttcatggc
       61 cgccgcgaag gtcggcggca tcgtcgcgaa cgacgcctat ccggccgact tcatccgcga
      121 caacctcgag atccaaacca acgtcatcga cgcggcgtac cgcaacggca cgcgcaagct
      181 ctgcttcttg ggctcgagct gcatctaccc gcgccttgcg ccgcaaccgc tcgaagaatc
      241 gtcgctgttg acggggccgc tcgagccgac gaatcagtgg tatgccgtcg ccaagatcgc
      301 gggcatcaag atgtgccaag cgtatgcgcg gcaatacggc ttcaacgcga tctccgtgat
      361 gccgacgaat ctttacggcc ccggcgacaa cttcgatttg caaacctcgc acgtgctgcc
      421 ggccctgctg cgcaagctgc acgctgcgaa gcaaaacggc gacgccgagg tcaccgtctg
      481 gggctcgggc acgccgcgcc gcgagttcct ctacgtcgac gatttggccg acgcgctgct
      541 gttcctcatg gagcgctacg agtcgccgga gatcatcaac gtcggctgcg gcgaggacgt
      601 cacgatcgcc gagctcgcgc ggctggtcgc cgagatcgtc ggcttccgcg gttcactggt
      661 tttcgatcgc agcaagcctg acggcacgcc gcgcaagctg ctcgacgtca gcaaaactcg
      721 cgcactgggc tggcaaccga agacgtcgct cgcggacggc attcgggcga cgtacgactg
      781 gtatctcgcg aacggcagcc aagcccgcgc cggataactg cgagctctca gaacacgctg
      841 acgcgcgccg cggtgccgcc gagccaccac gcgacctcgc tgaacgtgct caagtagctc
      901 gcgaacagcc gacgcgtgcg cgccaacagc agcatgtcga tgaggtcctc ggtgatgccg
      961 cgcggcgcga gccagctgtc ggctcgtgac gtcgtgcgcg gataacagag cacgcgcgct
     1021 gcgccgtaac gctcggacaa catcggcgca atgtcgtcgc tgtcgctgac gacgagaaaa
     1081 cgcgcgccgg ccccggcacc gtcgagcaag cccgtcaggc ggcgcacggc cggcaagtga
     1141 tatttgcggt ggcggcgagc atcgtcacgc cacgtgcgca cttgcacgcc gacgacgtcg
     1201 tcgtcgaggt tcgcggctgc ccagtgcgct acggccgcgg cgatctcggg acgcacggcg
     1261 attcgccgaa acagctcacc gtagacgccg cggaagtagt gtgggatgcg ctcgtactcg
     1321 aggtcgatgt gccgcgcgtc ggcgcgcgtc gagcggcgcg agtgctgctt cggaaacacc
     1381 atgaagcgat agcgatccgt gcgctcgccc gtcaggctcc accacgcctt accgaggccg
     1441 cgaacgagcg ggtgagcacc ggcgccgacc gtcgcaaagc cggccggcaa gtgcggctcg
     1501 tcttgcggca ggacggcgag ccgccacgag ccgtagacgg ccgccccgct cggcaccgcg
     1561 tcgatcgcac actcgttcac gaacaactcg ccgaacgccg cgggcatgtt cttcgtcacg
     1621 ggccaatgca cgcgggcgtc ggccgcgagc cgcatgctcg agacccatga tttcaagcga
     1681 ttgccgaggc cgcccgtttc cgtggagatg acgagatcgt taatgaccat gaacggcgag
     1741 cgcgactcgg gaagatggtc ggcgatctta ccaagcggca gccgacgcgt tcacgccgca
     1801 gcgcgttcga acggtgaact gtgcataccc gggcgcgacg cgcctgtggc actatcttgc
     1861 cggcgccgcc gcgcctaatc ggggttccta cgaggttgcc caccatgacc gccctttcga
     1921 ctcggcacgg catcgccggc gccgcgcttg ccgtttgcct tgtattcgcg acggcgccgc
     1981 tgcacgggca gccggacttc ggcgacaaca cgagtcagtg ggccaacgac ggcgaatgcg
     2041 acgacccgcg tttcgaaggt gccggttccg ccgacacgtt actcgacgag gatcgcggtc
     2101 acgacgcaac cgattgccgc acgttgttca cggccggccg catcacgctg cgcagcgcag
     2161 cggcgagcaa caacggcaac gtgaatttcg gcaacgactc gagccgctgg gcgcgggacg
     2221 gcgaatgcga cgacccacgc ttcgaaggcg aaggcagcgc cgacacgttg ctcgacgagg
     2281 acaacggtca cgacgcggcc gattgccggt ctctgtttca agccggccgc atcgcgctac
     2341 gcggcgccgc gagctcggtc gacttcggcg acgactcgag cgaatgggcg cgcgacggcg
     2401 agtgcgacga tccgcgcttt gagggcgacg gcagtgcgga gacgctgctc gaagccgacg
     2461 cctatcacga cgcgaccgat tgccgaacgc tgctgagcca aaatcgcatc tcgctacgtg
     2521 gcgacgccgc ggctacggac ctgaaccgcg gccggctcga gaaaggcgac gacacgttga
     2581 gctccggcga gtacatggac gcgtacacgt tcacgggcag cacgggccag cgcgccgtcg
     2641 tcgacctgcg ctccggcgac ttcgacccgt acgtcttcgt ccgcgcgccg agcggcaagc
     2701 agttcgacaa cgacgacttc gaaggcgacg cgaaccgctc gctgctcgcg ctcgatctga
     2761 ccgagagcgg tgagtacaaa gtcaccgtca cgacctacaa gaaggacgag acgggcggct
     2821 atacgctgtc gatcgacgtc ggctcgagct cgagtatcgc ttcccgtatc gatcgcaacg
     2881 gccgactcga aaacggcgac gtcacgctga cgagcggcga gttcgtcgac acgtacgagt
     2941 tcgaaggctc gccggggcaa cacgtcgcga tcgacttgcg ctcttcggcc tttgacacgt
     3001 atctgatctt gagggacccg gccggcgagc aaacggagaa cgacgacgcg accgagagca
     3061 ccgacgtcgg ccacagcagc atcgaagccg acttgaccga ggccggcacg tacaaggtgc
     3121 tcgtgacgag ctacgagacc ggtcagagcg gcgcctattc gttgacgatc gatcccgcgg
     3181 cgcagccgcg cggcgccccg gccacgcggg acgtcacgac gttgacggtc ggccgcacgg
     3241 tctccgggga cctaggcagc agcgatccga cgtacgaagc cggcgagtat cacgatacct
     3301 acgtcttcga cggcgacgaa ggcgaaaccg tgcgcatcga gctgacgtcg acggacttcg
     3361 acacctatct cggcctcatt acgccgtccg gcgaagaaat cgccaacgac gatttcgaag
     3421 gcgactcggc gcgctccgtg atcgcgctga cgctgtccga ggccggtcgc tatcgcgtgc
     3481 aagcgacgtc gtacggagcg gccgagacgg gccgctacgg cctcgagctc acgacgagca
     3541 cggccagcat cccgatcgag cgccggtcgc aaggccgcgt ctacggcttg ttcaccggta
     3601 tcagcgacta cccggggtcg gagagcgact tgaattacac ggcagaggac gcgacgcgaa
     3661 tccgcgacgc gctcgtgcgc ggcggcggaa tgcgcgccga ggacgccgtg acgctcgtcg
     3721 atggcgacgt cacggtcggc aacatgacgg ccgcgattcg cgacatcggg cggcgcatgc
     3781 agccccagga cacgttcgtg atgttctact cgggacacgg tggccaggtg cgacgctcgg
     3841 ccccgacgtc gacggacccc gacggcttgg acgagacgct cgcgctttac gacggggcgc
     3901 tgctcgacga cgaccttgcg tcgctattcg acgagatcca caacggcacg gtgctgctct
     3961 ggctcgactc gtgcttcagc ggcggcttcg cgaaggacat cgtgtcggcg ccggggcgca
     4021 tgggcatctt ctcgtcggag gaagacatca cgtcgaacgt cgcgagcaaa ttccgcgccg
     4081 gcggctatct gtcggcgttc ctcgacgagg ccattgcgca aggcctcgcc gacgacgaca
     4141 aggacagctc gatcacggcg atcgagctca gccagtacct gcacgagcgg tatcgcgccg
     4201 acgtcaagag cgcgcgcccc gtcgaggtcg tccgtaccga gatgacgctc ggctatcagc
     4261 acctcgtcgt ggaccgcggc agcatcggcg cctacgacgt gttgttcgag cgctaggcgc
     4321 gccgcaagcc gggagtagca agcgctgcgc cttggcgcag cgcctcaatg cggcgcgagc
     4381 tcgagacgcc gcggcaagtg ccagctgatc gcattcgccg ccaacgacgc gccgagcatc
     4441 atcgcggcga ccggcagcag cgtgccgtcg ttgagcatgc cgctgagcca gccgaacgtg
     4501 ccgccgagca ggaacatcat cgtcgtgtaa accgacgacg cgctgccggc cagctcgcgg
     4561 aaaaaaccca tgtagcgcga cgaacccgca gggccgacga ggcctagcgt cgcgacggcg
     4621 aggacgatga acggcacgac cgtgatcagc gcggcgtgcc ccgtcgcgat caccgtcagc
     4681 aggccggtga cgaccagcac ttggaccgtg agcccgacgc ggaagaacgt ccctgcgttg
     4741 tggcctttga ggcgccacat gctgaacaag ttcatcgaca tgaaaccgag cacgctcgtc
     4801 gcgaacagca tcgagaagcg cgccggagtg actccgaagt actgcatgta gatgaacgac
     4861 gagttcgtga cgaagatcat catgcaggac gccgagaacg ccatcgccat cgcgtagcga
     4921 atcggcaagt accgtgagtc cacgcgccgc cgcacgacct cgccgcattg ccgaaacacg
     4981 gagcgcagcg acaggctggc gaggcggccg ggccgagtct cgtgcacgag cacggagtac
     5041 gcgccgagca gcacggcgcc gtagaccgcc ttcagcacga agatcgtctc ccagccgagc
     5101 ggcagcaccg cggcgccgag cgccggcgcc acgagcggcg cgacgagcaa caccatcgta
     5161 acggtcgcga agcgccgacc cagctgctcg acgggataga tgtcgcgcac cgtggccaag
     5221 cagatcacgg tcgcaaagcc gccgccgatg gcctgaaaga atcgcagcgt cagcatttgc
     5281 ccgacgctcg ccgagaacgc gatcgccatc gacgtcacga cataaacggc gagaccggag
     5341 tagccgatgc gtttgcggcc gatctgatcg gacagcgcgc cgccgaagaa ttggccgatc
     5401 gcatagccga tcaggaacac gttcagcgtg ttctgcaggc tcacgacgct gacgccgaag
     5461 tctcgagcca tcgccggtat gcccggcaga tacgcgtcga tgctgagcgg ccccagcgcc
     5521 gcgagcgcgc cgaaaaagaa aatcagatac cagggcggag ttacccgtcc ggcggcgccg
     5581 gatgtggcgc gcgttacggt gtcggccatc agcgcggcac gtgcagcctg agataacgcc
     5641 acagcgtgcc ggcgaccaac gcgtgatcga tctcaccggc gacgagcagc tcttcgagat
     5701 ccgtgacggg cacgaggacg acctccgtga tttccgtccc ggtattttgc actgcctgct
     5761 cgggcacgac atcgactgca tagaacgcgt gtaggcggtt gccgaacagc gcgggattcg
     5821 gagcgacgac gccgagcggc aaggccgtgc gcgcgcgata gcccgtctcc tcgagacact
     5881 cgcgcagggc cgacaccgcc gggtcttcgc cgggatcgac caagccgccc ggaatctcga
     5941 gcgttactcg ccggttgcca tggcggtact ggcgcacgag caccacctgg ttgtccgccg
     6001 tgacgggcac gatctgggcc cagtcgacgg actcgatgcg atgaaatgtg cgcggcttgt
     6061 cgtcgacggg cgatgcggcg acggaacgtt cgacgttgaa gatacggcac tcggcgacgg
     6121 tcctgcgctc gagctcgcgc caggcgcaca cggtcgtcgg cggaagctgc ctcatgcggt
     6181 gcctaactga tagatcaaca gcgcgccgcc gtagccgagc gcggtcatgt agctccaggc
     6241 gaacagcggc caacgccagc tgttggtctc gcggcgaatc acggccaacg tcgccgcgca
     6301 ttgcaagcac caggcgtaga acagcagcag cccgagcacc atcggcaacg tgaacacggg
     6361 cgtgccgtcc acgtgctgcg cggcgcgcag acgcccggcc agagtctcct cctccgcttc
     6421 gccgccgacc gcgtagatcg tgcccagcac ggcaacgacg acctcgcgtg cggggaagcc
     6481 ggcgatgacg cccgccgaca cgcgccagtc ccagccgagt gggcggaaca cgggctcgac
     6541 ccagtgcccg acgcgcccga gccagctctg ctccaagtga ttggcccccg ccacgttgtc
     6601 gatgtcggcg agccgcgcgt cgagggcgtc acccgtcaac gatgccgtgg cctcggcctg
     6661 cgcagcggcg cgcgcctg
//
LOCUS       DATLEE010000621         6154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5949515, whole genome shotgun sequence.
ACCESSION   DATLEE010000621 DATLEE010000000
VERSION     DATLEE010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5949515"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VM692_13530"
     CDS             <1..287
                     /locus_tag="VM692_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HUQ53241.1"
                     /translation="LAAFGWTVAQPAVQALMSRSVPANEQGLLQGALASMTNLTSIAG
                     PPIWTGLFGYFVSEAAPVVVPGAAFFAAAGVFLVALLVAKRWFAASRTAG"
     gene            659..1765
                     /locus_tag="VM692_13535"
     CDS             659..1765
                     /locus_tag="VM692_13535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUQ53242.1"
                     /translation="MALAACGEPPPAAPGAFPPVDVGVVEVHPRALPLPLEYPAQLKG
                     VREVEVRARVSGILLERRYDEGAHVAAGDVLFRIDPDQFRAAVARARAEVAVQEASLG
                     QARRERDRIVPLYDQKLASLRERDTSLATFESAEAAVAAAQAALRTAELDLSYTEVRA
                     PIAGLTSREVRSEGSLVTAGAESSLLTYIVQADRLYVDLAVADDDAELVRDALANDAA
                     GVGVQVADAQGLAIGAMGKIEFVSPRIDDATGTVAIRAVLDNPGGIVPGRIVRAHIEG
                     VSVPGALVIPKRAVMHGAQGAYVWTVDASGQPAPAPVVLGALAGNDVVVTSGLMAGAR
                     VVVDGLLKIRPGQPVNPVPITESTAQNSESSRVP"
     gene            1762..4923
                     /locus_tag="VM692_13540"
     CDS             1762..4923
                     /locus_tag="VM692_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HUQ53243.1"
                     /translation="MISNFFIDRPLFATVVSAFIVIAGLAAFRELPVAMYPNVSPPQI
                     SVSTVYPGASAEVIAETVAAPLEQSLNGVEDMLYMLSGNSSTASSLSIALTFAVGTDP
                     DIALINVQNRVQSAIQLLPEEVRRQGVSIQKALPSFLMILTFDSPDGRYDDLFVSNYA
                     TLNIIDELRRIPGVGNVQVFGARDYSIRIWLRPDKLTELGLTPADVAAAVREQNAQSA
                     AGRLGEEPIHERVDLTLSVTTQSRLSDPEQFERIVLRAGEGGEVVRLKDVARVELGAR
                     DYGFALSRQGRPVIGAALQLAPDANALEAAAAIYAKMAELEPAFPEGVEWSIPYDTSN
                     YVRTSMREVALTFFEALILVFFVVWLFLYNLRATAIPSVAVPVSLVGTFAGMYLLGFS
                     INSLTLFGMVLAIGIVVDDAIVVLENVERHMRDEHSDPREATRRAMAEVSRPVIAIVL
                     VLNAVFLPVAFLGGLVGEMYRQFAITIAVSVTISGFVALTLTPAMCAKLLKSGGEEPK
                     GLLARFDKAFLRLTHAYTSGVRWTLRHGVVAVAAMAAMAAAIWWLDRATPSALAPSED
                     QGYVLALAALPPAASMQRTKAALDQLDQAAFAHPAFFDNITVTGFDVQTGGQRSNTGV
                     SFVLLKDWAERRKPELAADAVAGALMGAGAAIRDAFVFSLSPPAVEGMSNVGGLEGFV
                     QSRSNDYFALEQVAQTLVAAASQRPEFKNRDGSFNVATTYSAQVPRINVGVDIEKAKL
                     LGVSLDDINMTLQSTFGALYVNDFNREGRVYRVQMQSDAQYRAHPEDLRDVAVRSASG
                     AMIPLTALAAINQVTGPDLIERFNLFPSARLSGEPARGYSSGQALAAMEEVAAQVLPD
                     GYTLAWSGQSYQEKTSGQNTLAIFGLAVLMVFLILAAQYERITLPFAVILAVPFAVFG
                     AFFAVWLRGLYNDLYLQIGLVTLVGLAAKNAILIVEFAAHMHKEGMGLKEAAIEAARL
                     RFRPIVMTSLAFILGVLPLALSTGAGAASRHSIGTGVIGGMLAATFIAVLFIPQFFVW
                     TMRAGARVRRAFGLPETPQAIPE"
     gene            5198..>6154
                     /locus_tag="VM692_13545"
     CDS             5198..>6154
                     /locus_tag="VM692_13545"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ53244.1"
                     /translation="MIGGRPYTDRTTLIKGGYVLTLDAELGDLPLTDVLIDGDKIAAI
                     GRNLEVSPNANVIDARNRIVMPGFVDTHRHTWQTPVRGVLPSCTLDQYFSGMLDNIGI
                     QYRAEDVYIANLMGALECLNGGITTLLDWSHVNNTPEHADAAIAGLSEAGIRAVYAHG
                     PPVGASKWWAYSSAEHPEDVRRIRKQYFNSDDQLLTLALAARAPGNTTPDVAKHDWLL
                     ARDLGIRISVHVGMRLTGVHVNHVKTLNDLGLMGPDTTYIHCTDSTDEELDLIAKSGG
                     STSVAPYVEMLMGHGVPPTGRLVARGVRPSLSIDVVSSVPGDM"
BASE COUNT          945 a         2072 c         2068 g         1069 t
ORIGIN      
        1 cgctcgcggc tttcggctgg acagtcgcgc agccggccgt gcaagcactg atgtcgcgct
       61 cggtacccgc aaacgagcag ggcttgctgc agggcgcgct cgcgagcatg acgaacctga
      121 cgtcgatcgc ggggccgccg atctggaccg ggctgttcgg ctatttcgtg tcggaggccg
      181 cccccgtcgt cgttcccggt gcggcgtttt tcgccgccgc cggcgtgttt ctcgtggcgc
      241 tcctcgtcgc caagcggtgg tttgccgcgt cgcgcactgc gggctgacag tctcacgggc
      301 ctgccgaatc gccgctcgct agaagcgttc gagcgccggc gtcgccgaca cgacgatggc
      361 ccgcgcgttc gagcagcacg tgcgtatcgc ggacgaggcg ttgtatctcg cgaaacctga
      421 caatagctgt cacggatcgg ccccatgctg gcgcctgccg gctcgaaagc agggtgccga
      481 aaccgttaca attggatatt gccgacgcgt gctcggtgcc gcgttccgaa agccggccgc
      541 tcggccgccg ccctagatct ccgcgccgtc ctgcctgaag ggagtctgtt acgtgtcaat
      601 ccggtcaagt cgccgcaacg cgcggtgccg tagcggcttt ttcgcattgt tccccgcctt
      661 ggcgcttgcc gcgtgcggcg agccgccgcc tgccgcgccg ggtgcctttc cgcctgtcga
      721 cgtcggcgtg gtcgaggtac acccgcgcgc gttgccgttg ccgctcgaat acccggcgca
      781 gctgaaaggc gtgcgcgagg tcgaggttcg cgcgcgcgtc tcgggcatct tgctagagcg
      841 ccgctacgac gagggcgcgc acgtggcagc cggcgacgtg ctgttccgca tcgatcctga
      901 tcagttccgc gcggccgtag cgcgcgctcg ggccgaagtc gccgtgcaag aggcgagctt
      961 gggtcaggcg cgccgcgagc gcgatcgcat cgtgccgctc tacgatcaaa agctcgcgag
     1021 cctgcgggag cgcgacacgt cgctggcgac gttcgagagc gccgaagcgg ccgtcgccgc
     1081 cgcgcaagca gcactgcgca cggccgagct cgacttgtcg tacacggaag tgcgcgcgcc
     1141 gatcgcgggg ctcacgagcc gcgaagtgcg ctccgaaggc agcctcgtca cggcgggcgc
     1201 cgagtcgagc ttgctgacct acatcgtgca agcggatcga ctctacgtcg atctcgccgt
     1261 cgccgacgac gacgccgagc tcgtgcgcga cgcgctcgca aacgacgcgg cgggcgtcgg
     1321 cgttcaggtc gcggacgcgc aaggccttgc gatcggcgcc atgggcaaga tcgaattcgt
     1381 ctcgccgcgc atcgatgacg cgacgggtac cgtcgcgatc cgtgccgtgc tcgacaatcc
     1441 cggcggcatc gtgccgggca gaatcgtgcg cgcgcacatc gaaggcgtca gcgttccggg
     1501 cgcgctcgtg atcccgaagc gcgcggtcat gcacggcgcg cagggcgcgt acgtttggac
     1561 ggtcgacgcg agtggccagc ccgcgccggc gcccgtcgtg ctcggcgcgc tcgccggcaa
     1621 cgacgtcgtg gtcacgtcag gtctcatggc gggtgcgcgc gtcgtcgtcg acggtctgct
     1681 gaagattcgt cccggtcagc ccgtcaatcc ggtcccgatc accgaaagca cagcacagaa
     1741 cagcgagtcg agccgcgtac cgtgatttcg aacttcttca tcgaccggcc gttgttcgcg
     1801 acggtcgtct cggcgttcat cgtcattgcg gggctcgcgg cgtttcgcga gctgcccgtc
     1861 gcgatgtatc cgaacgtttc gccgccgcag atcagcgtct cgacggttta tccgggcgct
     1921 agcgcggaag tcatcgccga gaccgtcgcc gcgccgctcg agcagtcgct gaacggcgtc
     1981 gaagacatgc tctacatgct ctcgggcaac tcgtcgacgg cgagctcgct gtcgatcgcg
     2041 ttgacgttcg ccgtcggaac ggatcccgac atcgcgctca tcaatgtcca gaaccgcgtg
     2101 caaagcgcga tccagctctt gcccgaggaa gtgcgaaggc aaggcgtctc gattcagaag
     2161 gcgctgccga gcttcctgat gatcctgacg ttcgactcgc cggacggccg ttacgacgat
     2221 ctgttcgtct cgaattacgc gacgctgaac atcatcgacg agctcaggcg aattcccggc
     2281 gtcggcaacg tgcaagtctt cggcgcgcgc gattactcga tccgcatctg gctccggccg
     2341 gacaagctga cggagctcgg tctcacgccg gccgacgtcg ccgccgccgt acgcgagcaa
     2401 aatgctcagt ccgcggccgg ccggctcggt gaggagccga tccacgagcg cgtcgatctg
     2461 acgctctccg tcaccacgca atcgcggctc tcggaccccg agcagttcga gcgcatcgtg
     2521 ctacgcgccg gcgaaggtgg cgaggtcgtg cgcctcaagg atgtcgcgcg tgtcgagctc
     2581 ggtgcgcgcg actacggctt cgcgctctcg cgccaggggc gccccgtgat cggcgccgct
     2641 ctgcagctcg caccggacgc gaacgcgctc gaggccgcgg cggccatcta cgccaagatg
     2701 gccgagctcg agcctgcctt cccggaggga gtcgagtggt cgattccgta cgacacgagc
     2761 aactacgtgc gtacttcgat gcgcgaggtc gcgctgacgt tcttcgaggc cctgatcctc
     2821 gtcttcttcg tcgtctggct gtttctctac aatctgcgcg ccaccgcgat cccgtccgtc
     2881 gccgtgcccg tgtcgttggt cggcacgttc gcgggcatgt acctgctcgg tttctcgatc
     2941 aactctctga cgctgttcgg catggtgctc gcgatcggga ttgtcgttga tgacgctatt
     3001 gttgtgctcg agaacgtcga gcggcacatg cgcgacgagc actccgatcc gcgcgaagcc
     3061 acgcggcgcg cgatggcgga ggtcagccgc cccgtcattg cgatcgtgct cgtgctgaac
     3121 gccgtgtttc tgcccgtcgc gttcttgggc gggctcgtcg gcgagatgta tcggcaattc
     3181 gcgatcacga ttgccgtgtc cgtcacgatc tcgggcttcg tcgcgctgac gctgacgccg
     3241 gcgatgtgcg cgaagttact gaagagcggc ggcgaagagc cgaaagggct gttggcgcgc
     3301 ttcgacaagg cattcctgcg cctgacccac gcctacacga gcggagtgcg ttggacgctg
     3361 cgtcacggcg tcgtcgccgt ggcggcaatg gccgcgatgg cggcggcgat ctggtggctc
     3421 gatcgggcga cgccgagcgc gctggcgccg agcgaggacc agggctacgt gctcgcgctc
     3481 gcggcgctgc cgcctgcagc atcgatgcag cgaaccaaag ccgcgctcga tcagctcgac
     3541 caagccgcgt tcgcgcatcc ggcgttcttc gacaacatca ccgtcacggg tttcgacgtg
     3601 cagacgggcg gtcagcgcag caacacgggc gtcagcttcg tgctgctgaa ggactgggcc
     3661 gagcggcgca agcccgagct tgcggccgac gccgtcgcag gggcgctgat gggcgccggg
     3721 gcggcgatcc gcgacgcgtt cgttttctcg ctgtccccgc ccgccgtcga aggcatgtcg
     3781 aacgtcggcg ggttggaagg gttcgtgcag tcgcgctcga acgactactt cgcgctcgag
     3841 caagtcgcgc agacgctcgt cgccgcggcc tcgcagcgtc ccgagttcaa gaaccgcgac
     3901 ggcagcttca acgtcgcaac gacctactcg gcgcaggtgc cgcgtatcaa cgtcggcgtg
     3961 gacatcgaga aagcgaagtt gctgggcgtg agcctcgacg acatcaacat gaccctgcag
     4021 agcacgttcg gcgccctgta cgtcaacgac ttcaaccgcg aaggccgcgt ctaccgcgtg
     4081 cagatgcagt cggacgccca gtaccgcgcg catcccgagg atctgcgcga cgtcgccgtg
     4141 cgctccgcga gcggcgcgat gatcccgctg acggcactcg cggcgatcaa tcaggtcacg
     4201 ggcccggatc ttatcgagcg tttcaatcta ttcccgtcgg cgcggctctc gggcgagccg
     4261 gcgcgcggct atagctcggg ccaggcgctt gcggcgatgg aggaagttgc ggcgcaggtg
     4321 ctgcccgacg gctacacgct tgcgtggtcg gggcagtcgt atcaagagaa gacgagcggc
     4381 cagaacacgc tcgcgatctt cggtctcgcc gtgctgatgg tgttcctgat cctcgcggcc
     4441 cagtacgagc gcatcacgct gccgttcgcc gtgatcctcg cggtgccgtt cgccgtgttc
     4501 ggcgcttttt tcgcggtctg gctgcgcggc ctctacaacg atctgtattt gcaaatcggt
     4561 ctcgtgacgc tcgtgggcct cgcggccaag aacgcaattc tgatcgtcga gttcgcggcg
     4621 cacatgcaca aggaaggcat ggggctcaag gaagccgcca tcgaagcggc gcgcctgcgc
     4681 ttccggccga tcgtcatgac gtcgctggcc ttcatcttgg gggtgctgcc gctggcgctc
     4741 tcgacgggtg ccggtgccgc gagccgccac tcgatcggca ccggcgtcat cggcggtatg
     4801 ttggcggcga ctttcatcgc cgtgctgttc atcccgcagt tcttcgtctg gacgatgcgc
     4861 gccggcgcgc gcgtgcgtcg agcattcggt ttgccggaaa cgccgcaggc gattcccgag
     4921 taacggcgac tggtcacgac ccgagcggcc gcagtatcgt ggccgcatgc cggctcgctg
     4981 gctccatgtc gctttgcttg cggctgcaag tcccgcgggc ctcagcgcgt cgcagatcac
     5041 ggaccgcagc gcgcgccgcg atcgaggcgg cggcgcgcag aatcgccgtg ctagcatcga
     5101 ccgagagccg acggtccgct cgcgagctcg agcggtgact cgcgcgaaca acagcaataa
     5161 cgcaacggcg cgagccagtt agcgccagga ggcaacgatg atcggcggac gcccttacac
     5221 ggatcgtacg actctgatca aaggcggtta tgtcctgacg ctcgacgccg agctcggcga
     5281 tctgccgctg accgacgtgc tgatcgacgg cgacaagatc gcggccatcg ggcgcaatct
     5341 cgaagtcagc ccgaatgcca acgtcatcga cgcgcgcaac cgcatcgtca tgccgggctt
     5401 cgtcgacacg caccgccaca cgtggcagac gccggtccgc ggcgtgttgc cgagctgtac
     5461 gctcgaccag tacttctcgg gcatgctcga caacatcggc atccagtacc gcgccgagga
     5521 cgtctacatc gcgaacctga tgggcgcgct ggagtgcttg aacggcggca tcacgacgct
     5581 gctcgactgg tcccacgtca acaacacgcc cgagcacgcc gatgcggcga tcgcgggctt
     5641 atcggaagcg ggcattcgcg ccgtctacgc gcacggccca cccgtcggcg cgtcgaagtg
     5701 gtgggcgtac agctcggcgg agcaccccga ggacgtgcgg cgcattcgca agcagtactt
     5761 caactcggac gaccagctct tgacgttggc gctcgcggcg cgcgctcccg gcaacacgac
     5821 gcccgacgtc gccaagcacg actggctgct cgcgcgcgac ctcggcattc gtatcagcgt
     5881 gcacgtcggc atgcggttga cgggcgtgca cgtgaatcac gtcaagacgt tgaacgacct
     5941 cggcctcatg gggcccgaca ccacgtacat ccattgcacg gactcgaccg acgaggagct
     6001 cgacctcatt gcgaaatcgg gcggcagcac ttcggtcgcg ccgtacgtcg agatgctgat
     6061 gggccatggc gtgccgccga cgggccggct cgtcgcgcgc ggcgtgcggc cgtcgctgtc
     6121 gatcgacgtc gtgtcgagcg tccccggcga catg
//
LOCUS       DATLEE010000622         5447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2979149, whole genome shotgun sequence.
ACCESSION   DATLEE010000622 DATLEE010000000
VERSION     DATLEE010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2979149"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3448
                     /locus_tag="VM692_13550"
     CDS             <1..3448
                     /locus_tag="VM692_13550"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HUQ53245.1"
                     /translation="NDSGGNESNYSAVLVSTTTNGALLLGGGGGFFYLPNLNFTGNDS
                     FTYYVIDGTSQSNAATVTITVAPSAGNTPPVASNDSYPGAEDAPLDVNAATGVLANDT
                     DAQPGPLTAVLLSGASNGTVTLRADGSFTYTPNAGFVGDDTFQYRARDGSGAVSAPAA
                     VTLHINQSNDPPVAADDSYTTNEDQTLNVNANNGVLGNDTDPERDALTAVLVTGAASG
                     TLTLQPNGSFSFAPAAGFSGTVTFTYQADDSTVRGNTATVTITVTAVNDPPTAVADTY
                     TTTEEAALAVTAADGVLKNDTDSDAGTTLTAALIGNVANGTLTLRPDGSFTFTPASNF
                     SGPTTFTYQARDGAASSATTTVTINVTAVNDAPFISNAPPTTATEGVTYRYTLTASDP
                     DGNALQITAPTLPAWLRFTPPATIAGTPAQADAGVHDVTMEVSDGIAPAVVQRFQITV
                     QAVDNAPTISAVPAQTATEGAAFDFDLAKFVTDPDTAAGSLTYALTGGAPPGVTLSSA
                     GRLSGTPQLGASVGNYSVAFTVSDSTNHVSGQVAMTVIAAGRIDLAVTLSAAPSPVPL
                     DGPATWTISIANRAANVTAQGISLAASFNGDVPFRFDSPTTAGCTATPAGNQMGLSCT
                     LGPLAPGATTTITLTGSGGFAGDVFGHATVAATGGAIDEVPGNDRATASLSIAQRVSS
                     APEQRIVGVNARAVAAADINGDGFDDLAVATGSSQGLVLLPNIVDPTNSARRVLTPTP
                     QSLGGEALGNDVAFADLDRDGDLDIVVAAAAGAPDRAFLAASGAFTTAALGAPTLDSR
                     AVAVGDVNGDAFVDLVFANPGGSSLLVNSGSGGVFRAGPRIGAGDARDVLLVDLIGDA
                     LPELVIANGDGDATVYGNSGGVFTLARTLTTGPTSTVATGDFNGDSRADLVFGRDTAA
                     PPAIPTALVWLNGPANGGQLFVSDELGAATTSAVLVKDVDLEGSNDVLALNSAGVRIF
                     TNAAAANGTFVLHPQQLATPGARGFAAGRFSNDDRVDLAVVGADVTVFINDGKANFGS
                     GDSKAPTLTLRGEPTINITIDTPFVDPGATATDESDGDLTPRIVVAGKVDNALLGTYT
                     LTYSVTDLSGNAATPLTRTVNVQAQPQADEGGGGGAVGAELLLMLLASLVAQLRRTRA
                     VR"
     gene            complement(3445..4161)
                     /locus_tag="VM692_13555"
     CDS             complement(3445..4161)
                     /locus_tag="VM692_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53246.1"
                     /translation="MSNTLPFAVAIALGAVGAHAQEARSAHTQPDTSMGLYQPKQHEL
                     YDGRFVIRGTRVYQAGKLNDKSPWDHMGDDGSNLRAVDGEILLDVNELENTGTFRAEL
                     QLPEGRYVVELDRVHEFNPCQDGGIAAYLFEHGNSGCGDMNWPKSLLFVAGWGYGHAT
                     LNGQPLYQDYEIHFMVTQGMRDRESRKVMLAPSSGEAGAVNPATQQLDFYIRSPEQNS
                     ANHPQRAVFDHFFAMEVTWR"
     gene            complement(4172..5002)
                     /locus_tag="VM692_13560"
     CDS             complement(4172..5002)
                     /locus_tag="VM692_13560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ53247.1"
                     /translation="MTALRVAGKCIPGDYARTFLYLNLVYKPRVALRRLINTFYRMDH
                     VYAVMKEFKRAYKGPFSILEFGVADGYAFAKKAFATRYLDMEDSVVCHGFDSFEGLPA
                     TADRRDLGSVAGGDWKAGQYRGSYEELNDYLADRYRNCRLHRGMFDQTLTPAFLATLR
                     THKPILVWIDCDYYTSARVVLERLIPYLPSGCVIYFDEYEFNFGSRFTGESRLVHEIN
                     RGEFGDGIELVLDRELSWDLSRCYRFVSQTAPYAFERKTELRRVVQWRKHTGNDSPLP
                     "
     gene            complement(5185..>5447)
                     /locus_tag="VM692_13565"
     CDS             complement(5185..>5447)
                     /locus_tag="VM692_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53248.1"
                     /translation="AYDEPPAWFYTVRQSLGAALLRGGDGVAAERVFREALDTHPRDG
                     RLLFGLWQSLLKQGRGNEAVLVQEQFARAWAGATAQLTLDDL"
BASE COUNT          955 a         1893 c         1764 g          835 t
ORIGIN      
        1 taacgatagc ggcggtaacg agagcaacta cagcgccgtg ctcgtttcga cgacaacgaa
       61 cggcgcgctg ctgttgggtg gcggcggcgg cttcttttat ctgccgaatc tcaacttcac
      121 gggcaacgat tcgttcacgt actacgtcat cgatgggacg tcgcaaagca acgccgcaac
      181 ggtgacgatc acggtcgctc cgagcgccgg caacacgcca ccggtcgcca gcaacgactc
      241 ctatccgggc gccgaggacg cgccgctcga cgtcaacgcc gccaccggtg tgctcgcgaa
      301 cgacaccgat gcgcagcccg ggccgctgac ggccgtgctc ctctccggcg ccagcaacgg
      361 aaccgtgacg ctacgtgcgg acggctcctt tacttacacg cccaacgccg gcttcgtggg
      421 cgacgacacg ttccaatatc gcgcgcgcga cggcagcggt gcggtcagcg cgccggcggc
      481 cgtgaccttg cacatcaacc agtcgaacga tccgccggta gccgcggacg acagctacac
      541 cacgaacgaa gaccagacgt tgaacgtcaa cgccaacaac ggcgtgctcg gcaacgacac
      601 cgaccccgag cgcgacgcgt tgacggccgt gctcgtaacc ggtgccgcga gcggcaccct
      661 gacactgcag ccgaacggct cattcagctt cgcgccggcg gcgggcttca gcggcacggt
      721 gacgttcacc tatcaagccg acgacagcac cgtgcgcggc aacacggcga ccgtgacgat
      781 cacggtcacg gccgtcaacg atccgccgac ggctgttgcg gacacttaca cgacgaccga
      841 agaagctgcg cttgccgtca ccgccgccga cggcgtgctg aagaacgaca cggattccga
      901 cgccggcacg acgctgacgg ccgcgctcat cggcaacgtc gcaaacggga ctttgacgct
      961 gcggcccgac ggctcattta ccttcacgcc ggcctcgaac ttcagcggcc cgacgacgtt
     1021 cacgtatcaa gcccgcgacg gcgctgcgtc gagcgccacg acgacggtga cgatcaacgt
     1081 cactgccgtc aatgacgcgc cgttcatcag caacgccccg ccgacgacgg ccacggaagg
     1141 cgtcacctac cgctacacgc tgacggcatc cgatcccgac ggcaacgcgc tgcagatcac
     1201 ggcacctacg ctgcccgcgt ggctgagatt caccccgccg gctacgatcg cgggcacgcc
     1261 cgcgcaggcg gacgccgggg tccacgacgt cacgatggaa gtcagcgacg gcatcgcacc
     1321 agcggtcgtc cagcggttcc agatcaccgt gcaagccgtc gacaacgcgc cgacgatctc
     1381 ggccgttccg gcgcagacgg cgaccgaagg cgcggcgttc gacttcgacc tcgcaaaatt
     1441 cgtcacggat cccgacacgg ccgcgggcag tctgacctat gcgctgaccg gcggcgcgcc
     1501 gcccggcgta acgctgagca gcgcgggcag attgagcggc acgccccaac tcggcgcgag
     1561 cgtcggcaac tattcggtcg ccttcacggt gtccgactcg acgaatcatg tgtcggggca
     1621 agttgcgatg accgtcatag cggcgggccg catcgatctc gccgtcacgc tgagcgccgc
     1681 gccgagcccg gtcccgctcg acggtcccgc cacttggacg atttcgatcg cgaaccgcgc
     1741 ggccaacgtg acggcgcaag gaatttcgct cgcggcctcg ttcaacggcg acgtgccgtt
     1801 ccgcttcgac tcgcccacga cggccggctg caccgcgacg cccgcaggca accagatggg
     1861 cttgagctgc acgctagggc cgctggcccc tggcgcgacg acgacgatca cgctgacggg
     1921 cagcggcggc ttcgccggcg acgtgttcgg gcacgcgacc gtggccgcga cgggcggcgc
     1981 gatcgacgag gtgcccggca acgaccgagc gacggcgtcg ttgagcatcg cgcaacgagt
     2041 cagcagcgcg cccgagcagc gcatcgtcgg agtcaacgcg cgggcggtgg cagcggccga
     2101 tatcaacggt gacggcttcg acgacctcgc cgtagcgacc ggttcgtcgc aaggcctcgt
     2161 cctgttgccg aatatcgtcg acccgacgaa ctcggcgcga cgcgtgctca caccgacccc
     2221 gcaatcgctc ggcggcgaag cgctcggcaa cgacgtcgcg ttcgcggacc tcgatcgcga
     2281 cggcgatctc gacatcgtgg tcgccgccgc agccggcgca cccgaccgcg ccttcctcgc
     2341 ggcaagcggc gcgttcacga ccgcggcgct cggcgcgccg actctcgaca gccgcgccgt
     2401 cgccgtcggc gacgtcaacg gcgacgcatt cgtcgacttg gtgttcgcga accccggcgg
     2461 cagctcgctg ctcgtcaaca gcggctccgg cggcgtcttc agggccgggc cgcgcatcgg
     2521 cgccggcgac gcgcgcgacg tgctgctcgt cgacctgatc ggcgacgcgc tgccggagct
     2581 cgtcatcgcg aacggcgacg gcgacgcgac cgtctacggc aacagcggcg gcgtgttcac
     2641 gctggcgcgc acgctgacga cagggccgac gagcaccgtc gcgacgggcg acttcaacgg
     2701 cgacagccgc gccgatctcg tgttcggccg tgacacggcc gcgccgccgg cgataccgac
     2761 cgcgctcgtc tggctcaacg gcccggcgaa cggcggtcag ctgttcgttt ccgacgagct
     2821 cggtgccgcc acgacgagcg ccgtgctcgt caaggacgtc gacctcgagg gcagcaacga
     2881 cgtgctcgca ctgaattcgg ccggcgtgcg catcttcacg aatgcagccg ccgccaacgg
     2941 cacgttcgtg ttgcacccgc agcagctcgc aacgccgggc gcgcgcggct tcgcggccgg
     3001 cagattcagc aacgacgacc gcgtcgacct ggcggtcgtc ggcgccgacg tcaccgtctt
     3061 catcaacgac ggcaaggcca atttcggctc cggcgactcg aaggcgccga cgctgacact
     3121 gcgcggcgag ccgacgatca acatcacgat cgatacaccg ttcgtggacc ccggcgcgac
     3181 ggccacggac gagtccgacg gcgacctgac gccgcgcatc gtcgttgccg gcaaagtcga
     3241 caacgcgttg ctcggcacct acacgctgac gtattcggta acggatctat cgggcaacgc
     3301 cgcgacgccg ctgacgcgca ccgtcaacgt ccaagcgcaa ccgcaggccg atgaaggcgg
     3361 tggcggtggc gccgtcggcg ccgagctgtt gctgatgctg ctcgcgagcc tggtcgcgca
     3421 gctgcgtcga acgcgcgccg tgcgctaacg ccaggtcact tccatcgcga agaaatggtc
     3481 gaacaccgcg cgttgcggat ggttcgcgct gttttgctcg gggctgcgga tgtagaagtc
     3541 gagttgctgc gtcgcgggat tgacggcccc ggcctcaccg gacgacggcg ccagcatgac
     3601 cttgcgtgat tcgcgatcgc gcatgccctg cgtcaccatg aagtgaatct cgtagtcctg
     3661 atagagcggc tgaccgttca acgtcgcgtg gccgtagccc cagcctgcga cgaacaacag
     3721 gctcttcggc caattcatgt cgccgcaacc cgagttgccg tgctcgaaca gatacgccgc
     3781 gatgccaccg tcctggcacg ggttgaactc gtgcacgcga tcgagctcga cgacgtatcg
     3841 gccttccggc agctgtagct cggcgcggaa cgtgccggtg ttctcgagct cgttgacgtc
     3901 gagcagaatc tccccgtcca cggcgcgcag attgctcccg tcgtcgccca tgtgatccca
     3961 cggcgacttg tcgttcagct tgccggcttg atacacgcgt gtgccgcgga tgacgaatcg
     4021 gccgtcgtag agctcgtgct gcttcggctg atagaggccc atcgaggtgt cgggctgcgt
     4081 gtgcgcgctg cgcgcttcct gcgcgtgcgc gccgactgcg ccgagcgcaa tcgcaacggc
     4141 gaagggcaat gtgttgctca tggcaggtac ctcagggaag cggcgaatcg ttacccgtat
     4201 gcttgcgcca ttgcaccacg cggcgcaact cggtcttgcg ctcgaacgcg tacggcgcag
     4261 tttgactgac gaagcggtag cagcggctca aatcccacga tagctcgcgg tcgagcacga
     4321 gctcgatgcc gtcgccgaat tccccgcggt tgatctcgtg cacgagccgc gattcaccgg
     4381 tgaatctgct accgaagttg aactcgtact cgtcaaaata gatgacgcag ccgctcggca
     4441 agtacgggat gagccgctcg agcacgacgc gcgccgacgt gtagtaatcg cagtcgatcc
     4501 acacgaggat cggcttgtgg gtgcgcaggg tcgccaaaaa cgcgggcgtc agcgtctggt
     4561 cgaacatgcc gcgatgcagg cggcagttgc ggtagcgatc ggcgaggtag tcgttgagct
     4621 cctcgtagct cccgcgatac tggccggcct tccagtcgcc gccggcgacg ctgccgagat
     4681 cgcggcggtc ggccgtcgcc ggcaagcctt cgaagctgtc gaagccgtgg cagacgacgc
     4741 tgtcttccat gtcgaggtag cgcgtcgcga aggccttctt cgcgaacgca taaccgtcgg
     4801 cgacaccgaa ttctaaaatc gaaaacgggc ctttgtaggc gcgcttgaac tccttcatga
     4861 ccgcgtagac atggtccatg cgatagaacg tgttgatgag ccggcgcagc gcgacccgcg
     4921 gtttgtagac gaggttcagg tacaggaacg tgcgcgcgta gtccccggga atacacttgc
     4981 ccgccacgcg taaggcggtc atcaataagg acttatctcc gaaccggttc gccccggcct
     5041 ggccgtcgtg tgctcgcatt gttcccagca aatcttgcaa ggtccgggcc agacgcgagc
     5101 caaacggcct caatttttgt agcggccgcg cagatgaggt agcgcagctg acaataacgc
     5161 gagcggctcg ggccgcacac gtcgctacag atcgtcgagc gtgagttgcg ccgtcgcgcc
     5221 ggcccaggca cgcgcgaact gctcctgcac gagcacggct tcgttgccgc gcccctgttt
     5281 cagcaacgat tgccagagcc cgaacaataa gcggccgtcg cgcgggtggg tgtcgagcgc
     5341 ctcgcggaac acgcgctcgg ccgcgacgcc gtcgccgccg cgcagcaagg ccgcgcccaa
     5401 gctttgacgc accgtgtaga accaggccgg cggctcgtcg tacgcga
//
LOCUS       DATLEE010000623         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6331673, whole genome shotgun sequence.
ACCESSION   DATLEE010000623 DATLEE010000000
VERSION     DATLEE010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6331673"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VM692_13570"
     CDS             complement(<1..452)
                     /locus_tag="VM692_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53249.1"
                     /translation="MRQFVWFAGVAAIAGFDGSALAQQAAAIFPGQKGGQDTFGHYAV
                     VADWPKPLAQLPGHDAWTYGAAQSVFAASPDRVFVLLRGELPVIERPATRRLRDVAPS
                     LEFPVFRLPIRDATVASPPAGGAAGAMPPDGLEAWVEQGGVLGVDARW"
     gene            600..1727
                     /locus_tag="VM692_13575"
     CDS             600..1727
                     /locus_tag="VM692_13575"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HUQ53250.1"
                     /translation="MIRLGAALVWLIVAGAALGQRAANAPAVADAAVAKAMTAAARTL
                     LESVRGDPEFSERLRSYSMEDKLLLTLDAPEREKWAYWPTERSGLSFDLMHAKHRTLT
                     HELLWLLLSNRGYHKLLNIMQLENVLQPTSGTGFPRGIEDYSLTLFGEPSDTVPWAWR
                     FEGHHISLSITVVPGQGFTVTPTFLGSDPAEVTFGPLAGLRVLRVEEDLGLQLANSLT
                     AAQLRTAVVPGTPDWNAQGGFAYPGDVPWDIVAATILRDRSDWNTWKTELQPDGIAFK
                     DLNGAQQAALLGILDEIFTTYRPEIADTYRESLDLDGLRFAWIGGLKRSEPHYYRVQT
                     RDFLFEFDNVQGNANHIHEVWRSRAGDFGEDLLRRHRAAEH"
     gene            1806..>2175
                     /locus_tag="VM692_13580"
     CDS             1806..>2175
                     /locus_tag="VM692_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53251.1"
                     /translation="MAAQLVRPFFIAACLLWSCFAAAQELPDRGDALVTLGKTFAFYS
                     DRVTNLHDFLVWNARSREPVEPSPDCLAGLPAEQRAAFDHAREHYKVFATPAGTRLLL
                     ALRYRLAGFGDSGIVDGGAVD"
BASE COUNT          347 a          733 c          733 g          362 t
ORIGIN      
        1 tcccagcgcg cgtcgacgcc gagcactccg ccttgctcga cccaagcctc gagcccgtcg
       61 ggcggcatgg caccggccgc gccgccggcc ggcgggctcg cgaccgtcgc gtcgcggatc
      121 ggcaagcgga agaccggaaa ctcgaggctc ggcgcgacat cgcgcagccg ccgcgtcgcg
      181 ggccgctcga tgaccggcag ctcgccgcgc agcaacacga acacgcggtc cgggctcgcg
      241 gcgaacacgc tctgcgccgc gccgtaggtc caagcgtcgt gacccggcaa ttgcgcgagc
      301 ggcttcggcc agtccgcgac gacggcatag tggccgaacg tgtcctggcc gcctttttgg
      361 ccggggaaga tggctgcagc ttgctgcgcg agcgcgctgc cgtcaaagcc ggcgatagcc
      421 gcgacgccgg cgaaccaaac gaactggcgc atgcgtaaac cccctgggtg ccggtgttac
      481 ggtttcggtt cagccgaacc gtaacaccgg caccgtgccg atcctaacgc ggctcgatct
      541 cacggaatac acacgcgtgg tgtatcttcg agcgcggcag cagaggaagg ggagtggaca
      601 tgattcggct cggcgcggcg ctcgtgtggc tcatcgtggc gggagcggcg ctcgggcagc
      661 gtgcggcgaa cgcgccggcg gtcgcggatg ccgcggtcgc gaaggcgatg acggcggccg
      721 cgcgcacgct gctcgaatcc gtgcgcggcg atcccgagtt ctcggagcgg ctgcgctcgt
      781 actcgatgga ggacaagctc ctgctgactc tcgacgcgcc cgagcgcgag aagtgggcct
      841 attggccgac cgagcgctcc ggtctcagct tcgacctcat gcacgcgaag caccgcacgt
      901 tgacgcacga gctcttgtgg ctgctcttga gcaaccgcgg ttatcacaag ctgctcaaca
      961 tcatgcagct cgagaacgtg ctgcagccga cgtcgggcac gggcttcccg cgcggcatcg
     1021 aagattacag cctcacgctg ttcggcgagc cgagcgacac cgtgccgtgg gcttggcgtt
     1081 tcgaaggcca ccatatttcg ctgtcgatca cggtcgtgcc agggcagggc ttcaccgtga
     1141 cgccgacgtt cttgggctcc gacccggccg aggtcacgtt cggcccgctc gcgggcttac
     1201 gcgtgttgcg cgtcgaggag gacctcggct tgcagctcgc aaactcgttg acggccgcgc
     1261 agctgcgcac ggctgtcgtg cccggcactc ccgattggaa cgcccaaggc ggatttgcct
     1321 atcctggcga cgtgccgtgg gacatcgtcg ccgccacgat cttgcgcgac cgcagcgact
     1381 ggaacacgtg gaagacggag ctccagcccg acggcatcgc gttcaaggac ttgaacggcg
     1441 cccagcaggc ggcgttgctg ggcattctcg acgagatctt cacgacgtat cgccccgaga
     1501 tagccgacac gtaccgcgag tcgctggacc tcgacggctt gcgcttcgcc tggatcggcg
     1561 ggttgaagcg aagcgagccg cactactacc gcgtgcaaac gcgcgacttc ctgttcgagt
     1621 tcgacaacgt gcaaggcaac gcgaaccaca ttcacgaagt ttggcgcagc cgcgccggcg
     1681 acttcggcga ggatttattg cggcgccatc gcgccgccga gcactagcgc gaatgacggc
     1741 gcgacttcgc gcgacggccg cgcggttttc tcgtgcacca ggcgacctgt tgcggaggat
     1801 catgcatggc tgcgcagctg gttcggcctt tcttcatcgc ggcgtgcctg ctttggtctt
     1861 gcttcgcggc agcgcaagag ttgccggatc gcggcgacgc gctcgtcacc ttgggcaaga
     1921 cgttcgcgtt ctacagcgac cgcgtaacga atctgcacga cttcttggtc tggaacgcgc
     1981 gttcccgaga accggtggaa ccctcgccgg attgtctcgc cgggctaccc gcggagcagc
     2041 gcgcggcgtt cgaccacgcg cgcgagcact acaaggtatt cgcaacgccg gccggcactc
     2101 gcttgctgtt agcgctgcgc taccgtctcg ctggcttcgg cgactccggt atcgtcgatg
     2161 gcggcgcggt cgaca
//
LOCUS       DATLEE010000624         3172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_2233184, whole genome shotgun sequence.
ACCESSION   DATLEE010000624 DATLEE010000000
VERSION     DATLEE010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2233184"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VM692_13585"
     CDS             complement(<1..1333)
                     /locus_tag="VM692_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUQ53252.1"
                     /translation="MKSIQIRGARTHNLKNIDLDLPRDQLVVLTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIYDYLRLLYARAGIPRCPDHHEPLEAQNVSQMVDTVMALPQGTKIMLLAPVVQDRKG
                     EYKEKLDELKTQGFLRARIDGEVIELDDPPKLDKRKKHDIEVVIDRLAVKPDIQIRLA
                     ESFETALNLADGVALVAFIDDKKKPALAFSNKFACPICGYSIAELEPRLFSFNNPQGA
                     CPDCDGLGLRMFFDAARVVTHPNLSLAGGAIRGWDSNSTFYWMMISALAKHYKFETSV
                     PWEKLPEKVHKLVLYGNDGEKIQYKYENKKKGNKWAWQHAWEGIIPNMQRRFNETQST
                     AVREELGKYQSTQPCQSCGGTRLNKAARNVFVQERPLNEIANLSVTSALEFF"
     gene            complement(2061..>3172)
                     /locus_tag="VM692_13590"
     CDS             complement(2061..>3172)
                     /locus_tag="VM692_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53253.1"
                     /translation="PGQTNPRAWMLLLDDDAEQARIVATHARFTSAVGHDLETWLRLA
                     AARGEFAAIAADRLAANPHDIATLYVEQLLAPAEQRAAICERHTALSRTNPTDGDYRF
                     LADRCLPDFNERGAAFLKSYDFLPQSGWLAFAVGWEHALRARWQDALPPLELAYSRVP
                     PLAETIAPILARLRRMTTAGPPDLTDLVRGSAGLRFFVSIEAGRPTLPQEQAYADLYS
                     GRLVEAAAPGRSIEANDLARIVRLAAASDGAPPELVQRALRLPQDSVDPTTIFAVIGL
                     LLRERDGTDGIAALARRTVPPMAAALMDIVDAVDARDFRRVDRLLEVADPAMRGQVYA
                     MATVALGQATPRAWRDSAMRLLFVPERPYFAASSE"
BASE COUNT          512 a         1024 c         1046 g          590 t
ORIGIN      
        1 cgaagaactc gagcgcgctc gtgacgctca agttcgcaat ctcgttcagc ggccgctctt
       61 gcacgaagac attgcgcgcc gccttgttca agcgcgtgcc gccgcagctt tgacacggct
      121 gcgtgctctg gtacttaccg agctcctcgc gcacggccgt gctctgcgtc tcgttgaagc
      181 ggcgctgcat gttcgggatg atcccttccc acgcgtgctg ccacgcccac ttgttacctt
      241 tcttcttgtt ctcgtacttg tactggatct tctcgccgtc gttgccgtac agcacgagct
      301 tgtgcacttt ctcgggtagc ttctcccacg gcacgctcgt ctcgaacttg tagtgcttcg
      361 cgagcgccga gatcatcatc cagtagaacg tgctgttcga gtcccagccg cggatcgcgc
      421 cgccggcgag cgacaggttc ggatgcgtga cgacgcgcgc ggcatcgaag aacatgcgta
      481 gacctaagcc gtcgcagtcc gggcaggcgc cctgcggatt gttgaacgag aacaggcgcg
      541 gctcgagctc ggcgatgctg tagccgcaga tcgggcacgc gaacttgttc gagaacgcga
      601 gcgccggttt cttcttgtcg tcgatgaacg cgacgagcgc gacgccgtcg gctaggttga
      661 gcgccgtctc gaagctttcg gccagtctta tttgaatgtc gggcttcacg gcgagccgat
      721 cgatgacgac ctcgatgtcg tgcttcttgc gcttgtcgag cttcggcgga tcgtcgagct
      781 cgatgacttc accgtcgatg cgcgcgcgta gaaaaccctg cgtcttcagc tcgtcgagct
      841 tttctttgta ttcgcctttg cggtcctgca cgaccggcgc cagcagcatg atcttcgtgc
      901 cttgcggcag cgccatgacg gtgtcgacca tctggctgac gttttgcgct tcgagcggct
      961 cgtggtgatc gggacagcgc ggaatgccgg cgcgcgcgta tagcagtcgc aggtagtcgt
     1021 agatctcggt aaccgtgccg acggtgctgc gcgggttgtg gctcgtgctc ttctgctcga
     1081 tcgagatcgc cgggctcaag ccctcgatgt ggtcgacgtc gggcttctcc atcatcgaca
     1141 ggaactggcg cgcgtacgcg ctcaagctct cgacgtagcg ccgctggccc tcggcataga
     1201 gggtgtcgaa ggcaaggctc gacttgcccg acccggacag gcccgtcaga accacgagtt
     1261 ggtcgcgcgg caggtcgagg tcgatgttct tcaggttgtg ggtgcgggcc ccacggatct
     1321 ggatgctctt catatattta ggagtcgccg gttatgtcag gtccggccag gaccaagcac
     1381 tataagcccc ccgcaaagtc cggcgcaaaa cgcgggagtg cctgaagtat cgcgggcgaa
     1441 cacgccacgc tatgtcatga actcgagccg accggctcgc ggtcgtcggc cgcctcgcat
     1501 ttgaactcgc ggccgccgtc ggggaacgcg ccaaattgtg atcgtgcaat tacgttcgtg
     1561 gcgcagacgt cttctgttgc gctcgcacga cgatcgcatg ttgacagcga tcacgcgccc
     1621 ggcctacttt tcctctgaag tgcgaaacaa atggggcggc ggtgttgcgt aaatcgcaac
     1681 aaccccccgc gtgccgcgag cccctgagag tcggtcgtaa cacagctagg gagtgttccg
     1741 gaggggacga caatgttgga gctttcccgc agctcgatcc tactcggccg cgccggcgtc
     1801 aacgagttca acacggcgtc gacgctgtac gacttgctcg gccgcaacct ggtcgtggtg
     1861 cgcgcttgac cttttaaggg gggaggggcc acgccgcggc ggccgaccag ccgccgcggc
     1921 gcatcgccca aggagcgggc ggccaggagg ccgcccgact tcttattcga gcgaccctcg
     1981 cgtcacgcag cacacgagct ggccgcccgt aacggccgag ctcatcgccg ctctgcgtcc
     2041 atctctaccg gctcagtcgg tcactctgag ctcgcggcga aatacggccg ctccggcacg
     2101 aatagcaggc gcatcgcgct gtcccgccac gcgcgcgggg ttgcttgacc gagcgcgacc
     2161 gtcgccatcg cgtagacctg gccgcgcatg gcagggtcgg ccacttcgag caggcggtcg
     2221 acgcgccgaa aatcccgcgc gtcgaccgcg tcgacaatgt ccatgagcgc cgcggccatg
     2281 ggcggaacgg tccttcgcgc gagcgcggcg attccgtcgg tgccgtcgcg ttcgcgtagc
     2341 agcagtccga tgacggcgaa aatcgtcgtc ggatcaacgc tgtcctgcgg caatcgcagc
     2401 gcccgctgca cgagctccgg cggcgcgccg tcggacgcag cggccaagcg cacgatgcgc
     2461 gcgagatcgt tcgcttcgat cgaccggcca ggggcggcgg cctcgacgag ccgcccgcta
     2521 tagagatcgg cgtaggcttg ctcttgcggt agtgtcggcc gcccggcctc gatgctcacg
     2581 aaaaagcgca gccccgccga gccgcgaacg aggtcggtga ggtccggtgg cccagccgtg
     2641 gtcatgcgcc gcaggcgcgc gaggatcggt gcaatggtct cggccagcgg cggcacgcgg
     2701 ctgtacgcaa gctcgagcgg cggcaacgcg tcttgccaac gcgcgcgtag tgcgtgctcc
     2761 cagccgacgg cgaacgcgag ccaaccgctc tgcggaagga agtcgtagct tttgagaaaa
     2821 gcggcgccgc gctcgttgaa gtccggcaag cagcggtcgg ccaggaaacg atagtcgccg
     2881 tcggtggggt tcgtacgtga cagcgccgta tgtcgctcgc agatggcggc gcgctgctcc
     2941 gccggcgcaa gcaactgctc gacatagagc gtcgcgatgt catgcggatt ggcggcgagc
     3001 cggtccgctg cgatcgcggc aaattcgccg cgagccgccg ctagcctcag ccacgtctcg
     3061 agatcgtggc cgacggcgct ggtgaagcga gcgtgcgtgg cgacgatgcg cgcttgctcg
     3121 gcgtcgtcgt cgagcagcaa catccacgcg cgcggattcg tttgacccgg ac
//
LOCUS       DATLEE010000625         3343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6331706, whole genome shotgun sequence.
ACCESSION   DATLEE010000625 DATLEE010000000
VERSION     DATLEE010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6331706"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..758)
                     /locus_tag="VM692_13595"
     CDS             complement(237..758)
                     /locus_tag="VM692_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HUQ53254.1"
                     /translation="MPTRFVATALALALLAAPAFAVEQGDVAPPFKAVDFDGRSVEFP
                     AVAGGKPAIVVFWATWCGYCKAFMPYLKGIAADYAAQGVKVVAINAKEDARGGDPRAY
                     VRGLGFAPIAVANGDSIAAAYGVQYIPGLMIVDGKGNVAYRRPWTDLPAGRTVAELWD
                     QQVRAALDLLARR"
     gene            925..1725
                     /locus_tag="VM692_13600"
     CDS             925..1725
                     /locus_tag="VM692_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53255.1"
                     /translation="MSKEPRTRRALMLTVAALACGAAVSVVEAQQPDFTGLYESYRGA
                     PGERASGFGGPRAALPLTEEGKRRVEEYQKLAGPTRLNGATFCADYGVPTMMSLPGSY
                     PIEFIQKPNQLTIIFEVNNETRRIYIGDRQLPPEQRLPSRAGYSAGHWEGNVLVVKTT
                     DLLDGQDQTGSPFSDQATIDERFSLEPGANGAQVLTYKAKITDPVYYTAPVEIERRYQ
                     AYDGFIIPYGCQDEIWYELLDLRRQQLEAGKPVEARMSDVYKVREDRE"
     gene            1773..2201
                     /locus_tag="VM692_13605"
     CDS             1773..2201
                     /locus_tag="VM692_13605"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53256.1"
                     /translation="MSATIGKFFLSALALALALCGAVATAHHSAAQFDFAQRVTIEGT
                     VKEFNVTNPHTWAVVEITDDKRGTRDAQYEGHSASHFYRAGYTRDLAKPGDKIAILIA
                     PRRDGEDGGFIQAFTVNGKTIGFGGLSPETGKRTEGGGAN"
     gene            2311..3183
                     /locus_tag="VM692_13610"
     CDS             2311..3183
                     /locus_tag="VM692_13610"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HUQ53257.1"
                     /translation="MMLRSVLFVPGNKPSMLEKALGLRPDAFTPDMEDSVPAAEKANA
                     RETITKFVPRLAALGVPVVPRVNSLETEWLEADLAAVVGPQIFGISIGKVRTAADISA
                     ISHLVGTLERRAGMRIGTLKLLPWIETAEAIVNVSSICRASDRIVAVAFGGEDFTNDM
                     GVERLEDESQIAYARQALCVAARAAHVPALDTPYFKLRDLDGLRESSLRAKSIGFKGR
                     FAIHPEQLDAVNECFSPSAQEIAHAERVVAAYEEAEARGRASTSLDGWVIDVPVVKRA
                     RALLELARRARAER"
BASE COUNT          599 a         1131 c         1077 g          536 t
ORIGIN      
        1 agaatgttgc ggtcgttgag caccttcgtg cgcttgatgc tcgggtgatg aatacaggat
       61 tgagccaccg gagccggctc gtccgcgagc aacggcgagg ctgcgactac cgtggcgacg
      121 agcgtgaaca gaactctagc cggcgatgca ttcatggcgg ctgctccgaa cccgattggt
      181 tcttatatac gccgagcaag accgaagcga aagccaactc gagcggcgcg actccgctag
      241 cgacgcgcga gcaagtcgag cgccgcgcgt acctgctggt cccagagctc ggcgacagtt
      301 cgaccggccg gcaggtccgt ccacggacgt cggtatgcca cgttgccttt gccgtccacg
      361 atcatgagcc ccgggatgta ctgcacgccg taagcggctg cgatcgaatc gccgtttgcg
      421 acggcgatcg gtgcgaagcc caagccgcgc acgtaggcgc gcggatcgcc gccgcgcgcg
      481 tcttctttgg cgttgatcgc gacgaccttg acgccttggg ctgcgtagtc agcggcgatg
      541 cccttcaagt acggcatgaa ggctttgcag tagccgcacc acgtcgccca gaaaaccacg
      601 attgcgggct tgccgccggc gaccgcagga aactcgacgg agcgaccgtc gaaatcgacg
      661 gctttgaacg gcggcgcgac gtcgccttgc tcgacggcga atgccggggc ggcgagcagc
      721 gcgagcgcga gcgcggtggc gacaaatctc gttggcatgc tccctcttcg atcggctcgc
      781 gtcgagcggc ggtttatctt tcgccgggac tcgcgctgcg gtcaagcggc cgggccggtt
      841 gaaacatgag aaacgtgagg acgttttccc aagacacttg agaggcgcta cattgcgtgt
      901 cggagttccg aggaaaccct tgcaatgtcg aaagaaccgc gcacgcgccg cgccctgatg
      961 ctgacagtcg cggccctcgc ctgcggagcc gccgtctcgg tagtcgaggc acaacagccc
     1021 gatttcacgg gactctacga gtcttatcgc ggcgctcccg gcgagcgcgc atccggcttc
     1081 ggcggcccgc gcgcggcctt gccgctgacg gaagaaggca agcgccgcgt cgaggagtat
     1141 caaaagctcg ccgggccgac gcgactcaac ggcgccacgt tctgcgccga ttacggcgtg
     1201 ccgaccatga tgagcctgcc cgggtcgtac ccgatcgagt tcatccaaaa gcccaatcag
     1261 ctcacgatca tcttcgaggt caataacgag acgcggcgca tctacatcgg cgaccgacaa
     1321 ttgccgccgg agcagcgtct cccgagccgt gccggctact ccgcgggaca ttgggaaggc
     1381 aacgtgctcg tggtcaagac gaccgatctc ttggacggcc aggaccagac cggcagcccg
     1441 ttctccgacc aggccacgat cgacgagcgc ttctcgctcg agccaggcgc caacggcgcg
     1501 caagtgctga cctacaaggc caagatcacg gatcccgtct actacacggc gccggtcgag
     1561 atcgagcgcc gctatcaagc ctacgacggc ttcatcattc catacggctg ccaggacgag
     1621 atctggtacg agctgctcga tctacgccgt cagcaactcg aggccggcaa gcccgtcgag
     1681 gcgcgcatga gcgacgtgta caaggttcgc gaggaccgcg aatagtcaac cgtgtcgcgg
     1741 gcgttcgccc gcgcgtgatc gaggagatcg ttatgtcggc aaccatcggt aaatttttcc
     1801 tctcggcgct ggcgctggcg ctcgcgctct gcggagccgt cgcgacggct caccattcgg
     1861 cggcacagtt cgacttcgcg cagcgcgtca cgatcgaagg cacggtcaaa gaattcaacg
     1921 tcacgaaccc gcacacgtgg gccgtggtcg agatcaccga cgataaacgc ggtacgcgcg
     1981 acgcgcagta cgaaggccac agcgcgagcc atttctatcg cgccggctac acgcgtgatc
     2041 tcgcaaagcc cggcgacaaa atcgcgatcc tgatcgcacc gcgccgagac ggcgaggacg
     2101 gcggcttcat ccaagccttc acggtcaacg gcaaaacgat cggcttcggc ggtctgtcgc
     2161 cggagaccgg caaacgtacg gaaggcggcg gcgcgaacta gcgcgcgcgt tccgccggcc
     2221 gtaaccgttc agcgacggca ccgtcggtgc cgcgagcagc acgccgcagt gttaacctct
     2281 gcggcgtcgc tcttcgcgcg tgattgcccc atgatgcttc gcagcgtgct gttcgtgccc
     2341 ggtaacaaac cgagcatgct cgagaaagcg ctcggcttac ggcccgatgc gttcactccc
     2401 gacatggagg actccgtgcc ggccgccgag aaggccaacg cacgcgagac catcaccaag
     2461 ttcgtgccgc gcctcgcagc cctcggtgtg cctgtcgttc cgcgcgtcaa ctcgctcgag
     2521 accgaatggc tcgaagcgga tctggccgcc gtggtcggtc cgcagatctt cggcatttcc
     2581 atcggcaagg tgcgcacggc cgccgacatc agcgcgatct cgcatttggt cggcactctc
     2641 gagcggcgcg ccggcatgag gatcggcacg ctgaagctgc tcccgtggat cgagacggcc
     2701 gaggccatcg tcaacgtcag ttcgatctgc cgcgcgagcg accgcatcgt cgcagtggct
     2761 ttcggcggtg aggatttcac gaacgacatg ggtgtcgagc ggctcgagga cgaatcgcag
     2821 attgcgtatg cgcgccaggc gctctgcgtc gccgcgcgcg cggcgcacgt gcctgcactc
     2881 gacacgccgt acttcaagct gcgcgacctc gacggcttgc gcgagagctc gctgcgcgcg
     2941 aagagcatcg ggttcaaagg ccggttcgcg atccaccccg agcagctcga cgcggtcaac
     3001 gaatgttttt cgccgtcggc gcaagaaatc gcgcacgccg agcgcgtcgt cgccgcatac
     3061 gaggaagcgg aagcccgcgg ccgcgcttcg acatcgctcg acggctgggt catcgacgtg
     3121 ccggtcgtca agcgcgcccg cgcgctgctc gagctcgcgc gccgcgcgcg cgccgagcgc
     3181 tagcccgccg ccattcgcct cccgccgcgc atgcaatccg gcctgccgat cgacgaggcg
     3241 ctcgccccac tcgccgcggc actgcgctcg ggccgcaacg ccgtcgtcgt ggcgccgccc
     3301 ggcgccggca agagcaccgt cgtgcctctg gccctgctcg acg
//
LOCUS       DATLEE010000626         3988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8575073, whole genome shotgun sequence.
ACCESSION   DATLEE010000626 DATLEE010000000
VERSION     DATLEE010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8575073"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VM692_13615"
     CDS             <1..284
                     /locus_tag="VM692_13615"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HUQ53258.1"
                     /translation="FFAKMYPQLTDVAAKCKSLQDLLGTHQDACTATARLRRYAAARR
                     KQGEPAALPPALVELRKNQLAAARRARRTFRAEWAAFVAAIDATRQALA"
     gene            306..1136
                     /locus_tag="VM692_13620"
     CDS             306..1136
                     /locus_tag="VM692_13620"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HUQ53259.1"
                     /translation="MRLFAIGDIQGCAAPFDALLRKIAFRPSRDHLWLVGDLVNRGPE
                     SLAVLRRVMGLGPSVTCVLGNHDLHLLATVAGRRELSPADTFEDVLEAPDLNSIVDWL
                     RHRPLLHHDRDAKRLLVHAGIPAPWSAARARAEAREVETQLRGRKWRYALREMYGGEP
                     SKWSPKLRGVDRRRYTINALTRMRYCDRRGRLDLSYSGAPGTQPKGLIPWFDVPGRRA
                     AGTHIVFGHWAALGLYRRADVTALDTGCVWGNHLTAVRLDRPARAVRVSASVAATGRR
                     "
     gene            1184..1687
                     /locus_tag="VM692_13625"
     CDS             1184..1687
                     /locus_tag="VM692_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53260.1"
                     /translation="MSSATIARTFAVGAVTVAVALVLWTRSRAPGGDAPAISFADTDV
                     TPAAVATSPPDSPPSDAREATVAAKVAELDAMSETYRNTTFLIAIRDGGFVCNELLRV
                     YGALDDAPKWLVTCSEMLSYTVGVAGNGALHVEPMMQYFDGPVQTIQQVEPGPRPSQP
                     QVQPRLR"
     gene            complement(1684..2574)
                     /locus_tag="VM692_13630"
     CDS             complement(1684..2574)
                     /locus_tag="VM692_13630"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HUQ53261.1"
                     /translation="MSNLKTKIKAGQFVITAEIVPPLSAAGTKLLHEAGQLKGKVDAI
                     NLTDAAAGKTSISSFAAAALLVANGHEPILQVTCRDRNRIALAGDLIGAAAQGVENLL
                     ILRGDDPKGGDQPDAKPVFDFESIDLLKVARDMRDKGELPSARKIDSPPKFFIGAADV
                     PKRPDAKWSPVPLQKKIEAGADFIQTQFGFDLDVVRAYVERLSSEGITQKLGVIIGVG
                     PIRSAKSARWMNENLFGVTVPEPIIARLEGAADQAAEGRKVCAELIEGLREIKGVAGA
                     HVMAPAQGTDAIATVLDSLG"
     gene            complement(2586..3764)
                     /gene="pobA"
                     /locus_tag="VM692_13635"
     CDS             complement(2586..3764)
                     /gene="pobA"
                     /locus_tag="VM692_13635"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HUQ53262.1"
                     /translation="MGNQRTQVGIIGAGPSGLLLAQLLDRSGIDSVVLERRSRDYVLS
                     RVRAGVIEQGTVAILREAGVGTRLEREGLKHHGVEIATGEQRYRIDFAAQNASLTVYG
                     QTELTKDLMDARDASGKPPVFEVDDVVPEDVAGDRPRLRYRQTGAQRVLECDFIAGCD
                     GFHGVSRAAIPAHVLRTHERVYPFGWLGVLADAPPVSPELIYLSHERGFALCSMRSAA
                     RSRCYLQCELDTDVAAWSDERFWDELEARLPAPLARQLVRGPSIEKSVAPLRSFVAEP
                     LRHGRLFLAGDAAHIVPPTGAKGLNLAVRDVHQLAAALVTHYREKRDTLLDGYSASAL
                     RRIWRAERFSAWLTSVLHRFPGRTSFDRGMQIAELDQLVSSAAARAAFVEAYTGSQGE
                     "
     gene            complement(3769..>3988)
                     /locus_tag="VM692_13640"
     CDS             complement(3769..>3988)
                     /locus_tag="VM692_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016929667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HUQ53263.1"
                     /translation="IGATNDRRFRAFDARTGAELWVTELPLSAHAVPITYGGADGRQY
                     VAIVAAGQLAIDDLGAGDAQSLIAYALP"
BASE COUNT          641 a         1378 c         1322 g          647 t
ORIGIN      
        1 agttcttcgc aaagatgtat ccgcaactca ctgacgtcgc agctaaatgt aagtcgctgc
       61 aggatctcct cggcacgcac caagacgcct gcacggcgac ggctcgcttg cgccgctacg
      121 ccgcggcgcg gcgcaagcaa ggcgagccgg ctgcgctgcc accggcgctc gtcgagctcc
      181 ggaagaacca gctggcggcc gcgcggcgcg cgcgccggac gttcagagcc gagtgggccg
      241 cgttcgttgc ggcgatcgac gccacgcggc aggcccttgc ctagcgttac gcgccgtaac
      301 tgcacatgag gctattcgcg attggcgaca ttcagggctg cgctgcgccc ttcgacgcat
      361 tgttgcgcaa gatcgcgttc cgcccgtcac gcgaccacct ttggctcgtc ggcgatctcg
      421 tcaaccgcgg ccccgagtcg ctggccgtgc tgcgccgcgt catgggactc ggccctagcg
      481 tcacgtgcgt gctcggcaat cacgatttgc acctgctcgc gaccgtcgcg ggccgccgcg
      541 agctctcgcc ggccgacaca ttcgaggacg tgctcgaagc tccggacctg aattcgatcg
      601 tcgactggct ccggcatcgg ccgctgctgc atcacgatcg cgacgccaaa cgcttgctcg
      661 tgcacgcggg catcccggct ccgtggagcg cagcgcgagc gcgagccgag gcgcgcgagg
      721 tcgagacgca gctgcgcggc cggaagtggc gctatgcgct gcgcgagatg tacggcgggg
      781 agccttcgaa gtggagcccg aagctgcgcg gagtcgatcg gcgccgctac acgatcaacg
      841 cgctgacacg catgcgttat tgcgaccggc gcggccgact cgatttatcg tactcgggcg
      901 ctccgggcac tcagccgaaa ggcctgattc cgtggttcga cgtcccggga cgacgggcgg
      961 ccggcacgca tatcgtcttc ggccactggg ccgcgctcgg cttatatcgg cgcgccgacg
     1021 tcaccgcgct cgacacgggc tgcgtctggg gcaatcatct gacggccgta cgactcgatc
     1081 ggccagcgcg agccgttcgc gtcagcgcct ccgtcgcggc gacggggcgg cggtaaacgc
     1141 gcggcacgga gcaaaacccc tttcgggact gtcgaagcgc agcatgagct cggcaacgat
     1201 tgctcggact ttcgccgttg gcgcggtgac ggtcgccgtc gcgctcgtgc tctggactcg
     1261 cagccgcgca ccgggcggcg acgcgccggc aatctcgttc gccgacacgg acgtgacgcc
     1321 cgcagccgtc gcaacgagcc cgccggactc gccgccgagt gacgcgcgcg aggctacggt
     1381 cgccgcgaaa gtggccgagc tcgacgcgat gtcggagacc taccgcaata cgacgttttt
     1441 gatcgcgatt cgcgacggcg gcttcgtctg caacgagctg ctacgcgtct acggtgcgct
     1501 cgacgatgcg ccgaaatggt tggtcacctg cagcgagatg ctctcttaca ccgtcggcgt
     1561 cgcgggcaac ggcgccctgc acgtcgagcc gatgatgcag tacttcgacg gtccggtgca
     1621 gacgattcag caggtcgagc ccggcccgcg cccgtcgcag ccccaagtgc aaccgcgcct
     1681 gcgctagccg agcgagtcga gcaccgtggc gatcgcgtcc gtgccctgcg ccggcgccat
     1741 gacgtgcgcc ccggccacgc ccttgatctc tcgcaagcct tcgatcagct cggcgcaaac
     1801 cttgcgccct tccgccgcct gatcggccgc gccctcgagc cgcgcgatga tcggctcggg
     1861 caccgtgacg ccgaatagat tctcgttcat ccagcgcgcc gacttcgccg agcgaatcgg
     1921 accgacgccg atgatgacgc cgagcttctg cgtgatgcct tccgagctca gccgctcgac
     1981 gtaagcgcgc acgacatcca agtcgaagcc gaactgggtc tgaatgaaat cggcgccggc
     2041 ttcgatcttc ttctgcagcg gcacgggcga ccacttcgca tcgggccgtt taggcacgtc
     2101 ggcggcgccg atgaaaaact tcggcggcga atcgattttg cgcgccgacg gcagctcgcc
     2161 cttgtcgcgc atatcgcgcg cgaccttcag caaatcgatc gactcgaaat cgaacaccgg
     2221 cttcgcatcg ggctgatcgc cgcctttcgg gtcgtcgccg cgcagaatca gcagattctc
     2281 gacgccttgc gccgctgcgc cgatcaggtc gcccgcgagc gcgatgcgat tgcgatcgcg
     2341 gcaagtcact tgcaggatcg gctcgtggcc gttggcgacg agcagcgcgg cggccgcgaa
     2401 gctgctaatc gaggttttgc cggcggccgc gtccgtgagg ttgatcgcgt cgaccttgcc
     2461 cttgagctgt ccggcttcgt gcagcagctt cgtgccggcc gccgacaacg gcggcacgat
     2521 ctcggccgtg atgacgaact ggccggcttt gatcttggtc ttgagattcg acatgtcctg
     2581 ctccgtcact cgccttgaga ccccgtgtag gcttcgacga atgcggcacg cgcggccgcc
     2641 gagctcacga gctgatcgag ctccgcgatt tgcatgccgc ggtcgaacga cgttcgaccg
     2701 gggaagcgat gcagcaccga cgtgagccat gcggaaaaac gctccgcacg ccaaattcta
     2761 cgcaatgcgg aggccgagta gccatcgagc agcgtgtcgc gtttctctcg gtaatgcgta
     2821 acaagcgccg cggcgagctg atgaacgtct cgcaccgcga gattcaagcc tttcgctccg
     2881 gtcgggggaa cgatgtgcgc ggcatcgccc gcgaggaaca agcggccgtg tcgcaacggc
     2941 tccgccacga agctacgcaa cggcgcgacg cttttctcga tcgacggccc gcgcacgagc
     3001 tgccgcgcga gcggcgccgg cagacgcgcc tcgagctcgt cccagaagcg ctcgtcgctc
     3061 caagccgcga cgtcggtgtc gagctcgcac tgcaagtagc aacgactgcg cgccgccgag
     3121 cgcatgctgc aaagcgcgaa gccgcgctcg tggctcaaat agatgagctc cggagacacg
     3181 ggtggcgcgt cggcgagcac gccgagccag ccgaacggat aaacacgctc gtgcgtccgc
     3241 aacacgtgcg cagggatagc ggcgcggctc acgccatgga aaccgtcgca acccgcgatg
     3301 aagtcgcact cgagcacgcg ctgtgcgccg gtctgacggt agcgcagccg cggccggtcg
     3361 ccggcgacat cctcgggcac gacgtcgtcg acttcgaaca cgggcggctt gccgctcgcg
     3421 tcgcgtgcgt ccatgagatc cttcgtgagc tcggtttgac cgtagacggt caggctcgcg
     3481 ttctgcgccg cgaagtcgat gcgatagcgc tgctcgccgg ttgcgatctc gacgccgtga
     3541 tgcttgagac cttcgcgctc gagccgcgtg ccgacgccgg cttcgcgcaa gatcgcgacg
     3601 gtgccctgct cgatgacgcc ggcgcgcacg cggctgagca catagtcccg gctgcgccgc
     3661 tcgagcacga cgctgtcgat gccggagcgg tcgagcaatt gcgcgagcag caagcccgaa
     3721 ggccccgcgc cgatgatccc gacttgcgtg cgctggttgc ccatttggct acggcagtgc
     3781 gtaagcgatc agcgactgtg catcgccggc gcccaagtcg tctatcgcga gctgcccggc
     3841 ggcgacgatc gcgacgtact gccgcccgtc ggcgccgccg taggtgatcg gcacggcatg
     3901 cgccgacagc ggcagctccg tgacccaaag ctcggcgccg gtgcgtgcat cgaaggcgcg
     3961 gaaccgccgg tcgttcgtcg cgccgatg
//
LOCUS       DATLEE010000627         7891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6333606, whole genome shotgun sequence.
ACCESSION   DATLEE010000627 DATLEE010000000
VERSION     DATLEE010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6333606"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VM692_13645"
     CDS             complement(<1..227)
                     /locus_tag="VM692_13645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640290.1"
                     /note="catalyzes the transcription of DNA into RNA using
                     the four ribonucleoside triphosphates as substrates.
                     Dimerization of the alpha subunit is the first step in the
                     sequential assembly of subunits to form the holoenzyme;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HUQ53264.1"
                     /translation="MSAATEFLRPRAVKVQPLGTNRARVTVEPFERGFGHTLGNALRR
                     VLLSSMPGAAVVEAEIEGVLHEYTSIEGVQEA"
     gene            complement(247..867)
                     /gene="rpsD"
                     /locus_tag="VM692_13650"
     CDS             complement(247..867)
                     /gene="rpsD"
                     /locus_tag="VM692_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HUQ53265.1"
                     /translation="MARYRGPTCKLARREGTDLFLKSGLKPLESKCKLEVPPGGAGQR
                     RTRLSDYGVQLREKQKLRRMYGVLERQFRNYYKKATKVKGATGENLLRMLEARLDNVV
                     YRMGFASTRAEARQLVGHRGIKVNEQIVNIPSYQLKAGDKIAVRDRAKEQLRIKSALS
                     IAAQVGFPEWVEVDDKNMQGTLKNLPERDEILPDINENLVVELYSK"
     gene            complement(880..1290)
                     /gene="rpsK"
                     /locus_tag="VM692_13655"
     CDS             complement(880..1290)
                     /gene="rpsK"
                     /locus_tag="VM692_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HUQ53266.1"
                     /translation="MAATPPAAAAAAAKVRKKAKRQILDAVAHVHASFNNTIISITDR
                     QGNTLAWATAGNCGFKGSRKSTPFAAQVAAEKAGNQAKEYGVKSLEVRVKGPGPGRES
                     AVRALNAVGFRITEIEDVTPIPHNGCRPPKKRRV"
     gene            complement(1297..1662)
                     /gene="rpsM"
                     /locus_tag="VM692_13660"
     CDS             complement(1297..1662)
                     /gene="rpsM"
                     /locus_tag="VM692_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004154500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HUQ53267.1"
                     /translation="MARIAGVNIPTNKHVGIALTHIFGIGRNRALSVCKAAGVESTTA
                     VKDLTEPEIARIRDVIAKFPVEGDLRREVAMNIKRLMDLGCYRGIRHRRGLPLRGQRT
                     RTNARTRKGPRRGVKKMGG"
     gene            complement(1678..1791)
                     /gene="rpmJ"
                     /locus_tag="VM692_13665"
     CDS             complement(1678..1791)
                     /gene="rpmJ"
                     /locus_tag="VM692_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HUQ53268.1"
                     /translation="MKVRTSVRKICRKCKIVRRRGVVRVICEDPRHKQRQG"
     gene            complement(1799..3133)
                     /gene="secY"
                     /locus_tag="VM692_13670"
     CDS             complement(1799..3133)
                     /gene="secY"
                     /locus_tag="VM692_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HUQ53269.1"
                     /translation="MAKAGAVNPAALLGDATRSADLRHRLLFLLGALVVYRMGTFVPV
                     PGIDVAQFQQLFEQQGTILSMFNMFSGGALRRLSIFALGIMPYISASIILQMGTHVIP
                     ALQQLRKEGESGRRKITQYTRYGTVVLASFQAVAAASALTTQGVVLSPGPAFIMTAAI
                     TLLTGTMFLMWLGEQITERGIGNGISMLILSSILSGLPAAVGGTLELVNTGGMNQAMP
                     FLLGFLVLGVTGLVVYMERAQRRIAVNYAKRQQGRRMYAGQTSHLPFKINMSGVIPPI
                     FASSLILFPATIASWFGTSQSVSWLQRIAASLSPGQPVYILMYASLIMFFCFFYTALV
                     FNSRETAENLRRSGAFIPGIRPGQQTAEYIDKVLTRLTFWGALYITAVCLIPEFLILE
                     FNVPFYFGGTSLLIIVVVTMDFMAQLQAHMMTHQYEGLLKKANLRGYGKNEK"
     gene            complement(3141..3575)
                     /gene="rplO"
                     /locus_tag="VM692_13675"
     CDS             complement(3141..3575)
                     /gene="rplO"
                     /locus_tag="VM692_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HUQ53270.1"
                     /translation="MRLNDLHPAKGSKFAHKRRGRGGGSGLGKTGGRGVKGQRARAGG
                     YHKVGFEGGQMPLQRRLPKIGFVSRTRDDKAELRVHELAQFAGKTADMAAIKAAHLVP
                     KRVLTVKVIASGKLEQAVTVSGLKVTAGARKIIEAAGGKVVE"
     gene            complement(3577..3774)
                     /gene="rpmD"
                     /locus_tag="VM692_13680"
     CDS             complement(3577..3774)
                     /gene="rpmD"
                     /locus_tag="VM692_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HUQ53271.1"
                     /translation="MAKGTGTKNIRLTLLKSRFGRTPGHAQCAQGLGLRNRHQSVEVK
                     DTPENRGMIDKIRYMLRVEEA"
     gene            complement(3786..4274)
                     /gene="rpsE"
                     /locus_tag="VM692_13685"
     CDS             complement(3786..4274)
                     /gene="rpsE"
                     /locus_tag="VM692_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HUQ53272.1"
                     /translation="MANAEEFQEKLVAVNRVAKVVKGGRQFGFTALTVVGDGNGRVGY
                     GYGKARELPLAIQKAMQAARKHLTTVSLRKDTLQYELHGSHGATRVFMQPASDGTGVI
                     AGGAMRAVLECAGVRNVLAKSYGSRNPINVVRATMNALSAMRSPAAIAAKRGKTVEEI
                     LG"
     gene            complement(4287..4640)
                     /gene="rplR"
                     /locus_tag="VM692_13690"
     CDS             complement(4287..4640)
                     /gene="rplR"
                     /locus_tag="VM692_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HUQ53273.1"
                     /translation="MDKKAKRQRRARRTRVRISLQGVHRLTVHRTPQHIYAQIIAPEG
                     DRVVASASTLTKELRGSLKGTGNIAAAAAVGKEIAVRAKQAGIEKVAFDRSGFKYHGR
                     IKALADAAREAGLNV"
     gene            complement(4649..5182)
                     /gene="rplF"
                     /locus_tag="VM692_13695"
     CDS             complement(4649..5182)
                     /gene="rplF"
                     /locus_tag="VM692_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HUQ53274.1"
                     /translation="MSRVGKQPIELSKDLSAEVKGRSVTIKGKKGSLTLETSSEVEVT
                     VDGNIVKVVPRSKSTFANAMSGTTRAHLANMITGVTKGFEKKLELVGVGYRAAVQGKK
                     LNLTLGFSHPVEYPIPQGITIETPSQNEIIVKGMDRQQVGQVAAVLRHYRPPEPYKGK
                     GVKYSNERIELKEAKKK"
     gene            complement(5198..5593)
                     /gene="rpsH"
                     /locus_tag="VM692_13700"
     CDS             complement(5198..5593)
                     /gene="rpsH"
                     /locus_tag="VM692_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HUQ53275.1"
                     /translation="MSMTDPISDMLTRIRNAQTARKPSVTLPASKQKLAVAKVLEQEG
                     YLASVTSAEEDGKATLTLELKYYQDRPVIERIQRVSRPGLRIFKRVDELPVVQGGLGV
                     AIVSTSQGVMTNVQAKAQGHGGEVLCIVL"
     gene            complement(5605..5910)
                     /gene="rpsN"
                     /locus_tag="VM692_13705"
     CDS             complement(5605..5910)
                     /gene="rpsN"
                     /locus_tag="VM692_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HUQ53276.1"
                     /translation="MAKKSMLMRQRHREAAVKRYAAKRAELKELIRDPKTSDDSKAEA
                     QKKLQKLPRDANPIRLRNRCALTGRPHGYYRKFGLARTKLRETTMRGEIPGLRKASW"
     gene            complement(5913..6464)
                     /gene="rplE"
                     /locus_tag="VM692_13710"
     CDS             complement(5913..6464)
                     /gene="rplE"
                     /locus_tag="VM692_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HUQ53277.1"
                     /translation="MAAPRLQEFYRKEVVPALMKQLKVTNPMAVPRITKITLNMGVGE
                     ALADRKVLESAMGDLAKISGQKPVACKAKKSVASFKVRDNVAIGCKVTLREARMYEFL
                     DRLISVAMPRIRDFRGVSRRAFDGRGNYNLGVKEQIIFPEVTFDQVDAIRGLDISITT
                     TARTDDAGRALLEAFKFPLRGDR"
     gene            complement(6466..6780)
                     /gene="rplX"
                     /locus_tag="VM692_13715"
     CDS             complement(6466..6780)
                     /gene="rplX"
                     /locus_tag="VM692_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HUQ53278.1"
                     /translation="MQRIKKGDQVIVVAGKDKGRRGTVLRLVAGRVVVENLNVAKKHQ
                     RPNPQKGVAGGIVEQEKSLDASNVMLFNPTTQKADRVGYRRLSDGRKVRFFKGNGEVL
                     DT"
     gene            complement(6801..7169)
                     /gene="rplN"
                     /locus_tag="VM692_13720"
     CDS             complement(6801..7169)
                     /gene="rplN"
                     /locus_tag="VM692_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004145357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HUQ53279.1"
                     /translation="MIQTQTLLNAADNSGARSLMCIRVFGGSKRRYARIGDVIKVTIK
                     DAIPRGRVKKGEVYHAVVVRTRHGLRRPDGSLIRFDGNAAVLLTNKLEPIGTRIFGPV
                     TRELRTTAFMKIISLAPEVL"
     gene            complement(7179..7466)
                     /gene="rpsQ"
                     /locus_tag="VM692_13725"
     CDS             complement(7179..7466)
                     /gene="rpsQ"
                     /locus_tag="VM692_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HUQ53280.1"
                     /translation="MSAAETQGKVTHRPTVGRVVSAKMQKSVTVSIERLVKHPAYGKF
                     IRRTTKVMAHDEEGTCREGDTVAIVECRPISKRKAWRVVEVVQRAPVENAG"
     gene            complement(7478..7681)
                     /gene="rpmC"
                     /locus_tag="VM692_13730"
     CDS             complement(7478..7681)
                     /gene="rpmC"
                     /locus_tag="VM692_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HUQ53281.1"
                     /translation="MKVKEQRKAFRDKSDAELGEELLKLRREQFNLRMQAATGQGAKP
                     DQHGKVRKSIARLKTLQRERVKK"
     gene            complement(7681..>7891)
                     /gene="rplP"
                     /locus_tag="VM692_13735"
     CDS             complement(7681..>7891)
                     /gene="rplP"
                     /locus_tag="VM692_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019613682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HUQ53282.1"
                     /translation="FPDKPITKKPLEVRQGKGKGNVEYWVAQIKPGKLLYEIAGVTEA
                     VAREAFTLAAAKLPLSTTIVHRQGG"
BASE COUNT         1231 a         2592 c         2441 g         1627 t
ORIGIN      
        1 gcttcttgga cgccctcgat gctcgtgtac tcatgcaaca cgccttcgat ctcggcctcg
       61 acgacggcgg cgccgggcat cgaggacaac agcacgcgcc gcagcgcgtt gccaagcgtg
      121 tggccaaaac cgcgctcgaa cggctcgacc gtcacacgcg cgcgattcgt gccgaggggc
      181 tgaaccttga cggcccgcgg ccgtaggaat tccgttgctg cactcattaa aaggtttcct
      241 cctcggttac ttcgagtaga gctcgacgac caggttctcg ttgatgtccg ggagaatctc
      301 gtcgcgctcg ggcaggtttt tcagtgtccc ctgcatgttc ttgtcgtcga cctcaaccca
      361 ctccgggaag cccacctgtg cggcgatcga caacgccgac ttgatgcgca gctgttcctt
      421 cgcgcggtcg cggacggcga tcttgtcgcc cgccttcaac tgatacgacg ggatgttgac
      481 gatctgctcg ttgaccttga tgccgcgatg gccgacgagc tggcgcgctt cggcgcgcgt
      541 ggacgcgaag cccatgcggt acacgacgtt gtcgagccgc gcttccagca tgcgcagcag
      601 gttctcgccc gtcgcgcctt tgaccttcgt ggccttcttg taatagttgc ggaactgccg
      661 ctcgagcacg ccgtacatac ggcgcagctt ctgcttctcg cgcagttgca cgccgtagtc
      721 cgacaagcgc gtacgccgtt ggccggcgcc gccggggggt acttcgagct tgcacttcga
      781 ctcgagaggc ttgagcccgc tcttcaagaa caggtcagtg ccttcgcgac gcgccagctt
      841 acaagttggg cctctgtatc tcgccataat ttcttccgct taaacccgcc gcttcttcgg
      901 cggacgacag ccgttgtgag gaatcggagt gacgtcttcg atctcggtga tccggaaccc
      961 gaccgcgttc aaagcacgca cggccgactc gcgcccggga cccggaccct tgacgcggac
     1021 ctcgaggctc ttgacgccgt attctttcgc ctggttgccg gccttctcgg cggcgacctg
     1081 cgcggcgaac ggcgtgctct tacgcgagcc tttgaagccg cagttgccgg ccgtcgccca
     1141 cgcgagcgtg ttgccctgcc gatcggtgat gctgatgatc gtgttgttga aggacgcatg
     1201 gacgtgcgcc acggcgtcga ggatttgccg tttcgctttc ttgcgaacct tcgctgcggc
     1261 tgctgcggcg gcgggcggtg tagctgccat ttaaatctag cctcccatct tcttgacgcc
     1321 gcgacgcggg cccttgcgcg tgcgcgcatt ggtccgcgtg cgttggccgc gcagcggcaa
     1381 gccgcggcga tgccgaatac cccggtagca accgagatcc attaggcgct tgatgttcat
     1441 cgcgacctcg cggcgcaagt cgccctcgac ggggaacttc gcgatgacgt cgcggatgcg
     1501 cgcgatctcg ggctccgtca agtccttgac tgccgtggtc gactcgacgc ctgccgcctt
     1561 gcagacgctc agcgcgcggt tgcggccaat gccgaagatg tgggtcagcg caatcccaac
     1621 gtgcttgttg gtggggatgt tgacgcccgc tatacgtgcc atcgtgtgga tcctctatta
     1681 accctggcgc tgcttatggc gcgggtcttc gcaaatcacg cgcaccaccc cgcggcggcg
     1741 cacgatcttg cacttcctac aaatcttgcg aactgacgtt ctgactttca tctctcgcct
     1801 acttctcgtt tttgccgtag ccgcggaggt tggctttctt cagcagccct tcgtattggt
     1861 gcgtcatcat gtgagcctgg agctgcgcca tgaagtccat cgtcacgacg acgatgatca
     1921 gcagcgatgt gccgccgaag tagaacggca cgttgaactc caagatcagg aactcgggaa
     1981 tcaagcacac cgccgtgatg tacagcgcgc cccagaacgt gaggcgcgtc aacaccttgt
     2041 cgatgtactc ggccgtttgc tggccggggc ggatgccggg gatgaacgcg cccgagcgcc
     2101 gcaagttctc ggccgtttcg cgcgaattga acacgagcgc cgtgtagaag aagcagaaga
     2161 acatgatcag cgacgcgtac atcaagatgt aaaccggctg acccggcgac aggctcgccg
     2221 cgatgcgctg cagccacgac acggactggc tcgtgccgaa ccagctcgcg atcgtcgcgg
     2281 ggaacaggat caagctcgac gcgaagatcg gcgggatgac gcccgacatg ttgatcttga
     2341 acggcaggtg gctcgtctgg cccgcgtaca tgcggcggcc ttgctgacgc ttcgcgtaat
     2401 tgaccgcgat gcgccgctgc gcgcgctcca tgtagacgac gaggcccgtc acgccgagca
     2461 cgaggaagcc gagcaggaac ggcatcgcct gattcatgcc gccggtgttg acgagctcga
     2521 gcgtgccgcc gacggcggcc ggcaagccgg acagaatgct cgacaggatc agcatcgaga
     2581 tgccgttgcc gataccgcgc tccgtgatct gctcgccgag ccacatcagg aacatggtgc
     2641 cggtcagcag cgtgatcgcg gccgtcatga tgaacgccgg gcccggactc agcacgacgc
     2701 cttgcgtcgt cagcgcgctg gcggcggcca cggcttggaa cgatgccagc acgacggtgc
     2761 cgtagcgcgt gtactgcgtg atcttgcggc gacccgattc gccttccttg cgcagctgct
     2821 gcagcgccgg gatgacgtgc gtgcccatct gcagaatgat cgacgccgag atgtacggca
     2881 tgattccgag cgcgaagatc gacagccggc gcagcgcccc gcccgagaac atgttgaaca
     2941 tgctcaggat cgtgccctgc tgctcgaaca gctgctgaaa ttgcgcgacg tcgatgccgg
     3001 gcacggggac gaacgtgccc atgcggtaca cgaccaaggc gcccagcaaa aacagcaggc
     3061 gatggcggag atcggcggaa cgcgtcgcgt cgccgagcaa ggctgctgga ttgaccgcgc
     3121 ctgctttcgc catctctaac ctactccact accttgccgc cggcggcttc gatgatcttg
     3181 cgtgcgccgg ccgtgacctt gagcccgctg accgtgacgg cctgctcgag cttgcccgac
     3241 gcgatgacct tgaccgtcaa gacgcgcttc ggcaccaaat gcgcggcttt gatcgcggcc
     3301 atgtcggccg tcttgcccgc gaactgcgcc agctcatgga cgcgcagctc ggccttgtca
     3361 tcgcgcgtgc gcgacacgaa gccgatcttc ggcaggcgcc gttgcagcgg catctggccg
     3421 ccctcgaagc cgaccttgtg gtaaccgccg gcgcgcgcgc gctggccttt gacgccgcgg
     3481 ccgccggtct tgccgaggcc cgagccgccg ccgcgcccac gacgcttgtg agcgaacttg
     3541 ctgccctttg cgggatggag gtcgttcaag cgcatatcaa gcctcctcga cgcgcagcat
     3601 gtagcgaatc ttgtcgatca tgccgcgatt ctcgggcgtg tccttgacct cgacgctctg
     3661 atgccggttc cgcagcccga gcccctgcgc gcactgggcg tgacccggcg tgcggccgaa
     3721 gcggctcttc agcagcgtca agcggatgtt cttggtgcct gtgccttttg ccacgtcgaa
     3781 atcctttagc ccaagatctc ttcgaccgtc ttgccgcgct tcgcggcgat ggccgccggc
     3841 gaacgcatgg ccgacagcgc attcatcgtc gcgcgcacga cgttgatcgg attgcgcgag
     3901 ccgtagctct tcgcgagcac gttgcgcacg ccggcgcact cgagcaccgc gcgcattgca
     3961 ccgcccgcga tgacgcccgt accgtcggaa gccggctgca tgaacacgcg cgtcgcgccg
     4021 tggctgccgt gcaattcgta ctgcagcgtg tcctttctga ggctcacggt ggtcaggtgc
     4081 ttgcgcgccg cctgcatggc cttctggatc gcgagcggca gctcgcgcgc cttgccgtag
     4141 ccgtacccga cgcggccgtt gccgtcgccg acgaccgtca gcgccgtgaa gccgaattgc
     4201 cggccgcctt tgacgacctt cgcaacgcgg ttcaccgcaa ccaacttctc ttgaaactct
     4261 tcagcgtttg ccatgtgtca gctcgattaa acgttgaggc ccgcttcacg cgccgcgtcc
     4321 gcgagcgctt tgatgcggcc gtgatacttg aaccccgagc ggtcgaacgc gaccttctcg
     4381 atgccggcct gcttggcgcg gacggcaatt tccttgccga cggcggctgc ggccgcgatg
     4441 ttgcccgtgc ccttcaagct gccgcgcagc tctttcgtga gcgtcgacgc gctcgcaacg
     4501 acgcgatcgc cttcgggcgc gataatctgc gcatagatgt gctgcggcgt acggtgcacg
     4561 gtcagccgat ggacgccctg cagcgagatg cgcacgcgtg tacggcgggc gcgacgctga
     4621 cgctttgcct ttttgtcgat cgccatggct acttcttctt cgcttctttg agctcgattc
     4681 gctcgttcga gtacttgacg cccttaccct tatacggctc cggcggtcgg taatggcgca
     4741 gcacggcggc gacttgcccg acctgctgtc ggtccattcc cttcacgatg atttcgttct
     4801 ggctcggcgt ctcgatcgtg atgccctgcg gaatcgggta ctcgacggga tgcgagaaac
     4861 cgagcgtcag gttcagcttc ttgccctgca cggccgcgcg ataaccgacg ccgacgagct
     4921 cgagcttctt ctcgaaccct ttcgtgaccc cagtgatcat gttcgcaaga tgcgcgcgcg
     4981 tcgtgccgct catcgcgttc gcgaaggtgc tcttcgagcg cggcaccacc ttgacgatgt
     5041 tgccgtcgac cgtgacctcg acctcggagc tcgtctcgag cgtcaggctg cccttcttgc
     5101 ccttgatcgt gacggaccgg ccctttactt cggcgctcag gtctttgctg agctcgatgg
     5161 gctgtttacc tactcgcgac atcgtggtga ctcgtgatta aaggacgatg cagagaactt
     5221 cgccgccatg gccctgagcc ttggcttgca cgttcgtcat gacgccctgc gacgtggaga
     5281 cgattgcgac gccgagaccg ccctgcacga cgggcagctc gtcgacgcgc ttgaagatgc
     5341 gcagtccggg acggctgaca cgctggatgc gctcgatgac gggccggtcc tggtagtact
     5401 tgagctcgag cgtcagcgtc gccttgccgt cttcctcggc cgacgtcacg gatgcgagat
     5461 agccctcttg ctcgagcact ttggccacgg ccagcttctg cttcgacgcc ggcaacgtga
     5521 ccgacggctt gcgggccgtt tgcgcattac ggatgcgcgt cagcatgtcg gaaatcgggt
     5581 cggtcatact catgacggca tcctttacca gctggccttg cgcaagcccg ggatctcgcc
     5641 gcgcatcgtg gtctcgcgca gcttggtgcg tgcgagcccg aacttgcggt agtagccgtg
     5701 cgggcgtccc gtcaacgcgc agcgattgcg caagcggatc ggattcgcat cgcgcggcag
     5761 cttctgcagc ttcttctgcg cctcggcctt gctgtcgtcc gaagtcttcg ggtcgcggat
     5821 gagctccttg agctcggcac gcttggcggc gtaacgcttg accgccgctt cgcgatggcg
     5881 ctggcgcatc agcatggatt tcttggccat gattaacgat cccctcgcag cgggaacttg
     5941 aaagcctcga gcaacgcgcg gcccgcatcg tcagtgcgcg cggtcgtcgt gatcgagatg
     6001 tcgaggccgc ggatggcgtc gacttggtcg aaagtgactt ccggaaaaat gatctgctct
     6061 ttgacgccga ggttgtagtt acctcgaccg tcgaacgcgc gccgcgacac accgcggaag
     6121 tcgcggatac gcggcatggc gacgctgatg agccgatcca agaactcgta catccgggct
     6181 tcgcgcagcg tgaccttgca gccgatcgcg acgttgtcgc ggactttgaa gctcgcgacg
     6241 gacttcttgg ctttgcacgc gacgggcttc tggccgctga tcttcgcgag gtcgcccatg
     6301 gcgctctcga gcactttgcg gtctgccagc gcttcgccga cacccatgtt cagcgtgatc
     6361 ttcgtgatcc gcggaaccgc catcggattc gtgactttca gctgcttcat gagcgccggc
     6421 acgacttcct tgcggtagaa ctcttgtagt cttggtgctg ccatatcagg tatccaacac
     6481 ttcgccgttg cccttgaaga accgcacctt gcggccgtcc gacaaacggc ggtaaccgac
     6541 gcgatccgcc ttttgcgtcg tcgggttgaa caacatcacg ttcgacgcgt cgagcgactt
     6601 ctcctgctcc acgatgccgc ccgcgacgcc cttctgcgga ttggggcgtt gatgcttctt
     6661 cgcgacgttc aagttctcga cgacgactcg acccgcgacg agacgcagca ccgtgccgcg
     6721 ccggcccttg tccttaccgg cgactacgat gacttggtcg cctttcttga tgcgctgcat
     6781 gttcgttgct ccccgattgc ctacagcact tccggcgcca gcgagatgat cttcatgaac
     6841 gccgtcgtgc gcagctcgcg cgtgaccggc ccgaagatgc gcgtgccgat cggctcgagc
     6901 ttgttcgtga gcagcacggc ggcattgccg tcgaaacgga tcagcgagcc gtcggggcgg
     6961 cgcagaccgt gccgggtgcg gacgacgacg gcgtgataca cctcgccctt cttgacgcgg
     7021 ccgcgcggaa tcgcgtcctt gatcgtgacc ttgatgacgt cgccaatccg cgcatagcgg
     7081 cgcttcgagc cgccgaatac gcgaatgcac atcaggctgc gcgcgccgct gttgtcggcg
     7141 gcattcaata gggtttgagt ttgaatcatg gcggcacctt aaccggcgtt ctccaccggc
     7201 gcgcgctgga cgacctcgac gacgcgccag gctttacgct tgctgattgg gcggcattcg
     7261 acgatcgcga ccgtgtcgcc ttcgcggcac gtgccttcct cgtcgtgggc catgaccttc
     7321 gtcgtgcggc gaatgaactt gccgtacgcc gggtgcttga ccagccgttc gatggacacg
     7381 gtgacgctct tttgcatctt cgcgctgacg acgcggccga ccgtcggtcg gtgcgtgact
     7441 ttgccttgag tctctgctgc gctcatgtct cttctcgcta tttcttcacg cgctcacgtt
     7501 gcaaggtctt caagcgcgca atcgatttac gcaccttgcc gtgctggtcg ggcttcgcgc
     7561 cctggccggt cgccgcctgc atgcgcaggt tgaattgctc gcggcgcagc ttcagcagct
     7621 cctcgccaag ctccgcgtcc gacttgtctc ggaacgcctt tctctgttct ttgactttca
     7681 tcagccgccc tgccggtgga cgatcgtcgt gctcaagggc agcttcgccg cggcgagcgt
     7741 gaacgcctcg cgggcgaccg cttcggtcac gccggcgatc tcgtagagca acttgccggg
     7801 cttgatctgg gcgacccagt actcgacgtt gcccttgccc ttgccttgcc gcacttcgag
     7861 cggcttcttg gtgatcggct tgtcggggaa c
//
LOCUS       DATLEE010000628         4685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8576182, whole genome shotgun sequence.
ACCESSION   DATLEE010000628 DATLEE010000000
VERSION     DATLEE010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8576182"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..457
                     /locus_tag="VM692_13740"
     CDS             29..457
                     /locus_tag="VM692_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53283.1"
                     /translation="MSFREKSAWITLITLLLVSVLWSLHFPWGRPFTLAPGSDSFVFH
                     ALVLCTISFVVIVIVAHVVVAIRAPREASARADEREQLIGLKATRLAAYVYAALSMSS
                     VFLIHLGANEIGLAYFLVASLVIAESISCIARIVYHRRGV"
     gene            461..697
                     /locus_tag="VM692_13745"
     CDS             461..697
                     /locus_tag="VM692_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUQ53284.1"
                     /translation="MSQTTISNTIRRLRFERNEMTQQDLAERVGVTRQTINAIEAGKY
                     SPSLEVAFRIAGALGVRLEDVFSYDAGARPGEKP"
     gene            739..1371
                     /locus_tag="VM692_13750"
     CDS             739..1371
                     /locus_tag="VM692_13750"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HUQ53285.1"
                     /translation="MPPQWSAARIATIAAAHDEAVAAADPSDVGGGGTSLRVWDFVNR
                     GAVFDEIYLLVPVLAACRLVIGAQFKLSTMHSRAIKPHVRADTLHVDFAREATDAARG
                     AWPMLGFIVMLDDFTAANGATRFVPCSQHAAERPPCGEPVPACGPAGSVIVYNGSVWH
                     GHGANTTDRPRRSIQGAYIRRDAVGFAIAARMRPETLDRIGPLARQLLAV"
     gene            1406..1969
                     /locus_tag="VM692_13755"
     CDS             1406..1969
                     /locus_tag="VM692_13755"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HUQ53286.1"
                     /translation="MTVYVSMLRAVNVGGTSRIKMDALRAVYESIGLKDVRTLLQSGN
                     VVFRSAITDRAQLVKRIEQEIERQLELQVDVIVRTLAEIASIVERGPALSPKADMAKL
                     LVMFLAGVPDAAGQAALVQWHKERKGPEMLELRGPEIYLYYPDGVGRSKLSGAVIENK
                     LNTSGTARNWHTLQKLLETGRALEAQA"
     gene            complement(1966..2904)
                     /locus_tag="VM692_13760"
     CDS             complement(1966..2904)
                     /locus_tag="VM692_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53287.1"
                     /translation="MSVKDKLRGLGRHRGVRYAVSLPERLVRSASAIAAGTVREVAAV
                     ALPIGFRRGRLYRSLVDVTLQFLIEDVGGVQGGTPSDEKLGEDFLLRRSVGNGIELMG
                     VIAFRASPVWVLAALADVAGFGRQLIPEIADSLKKEGLLAQDGSFATMEQLLTGLEQS
                     SGQLAENVNAPPLDVKGLRAEWAKFVAGAKQLPAPQLPSPSSVTRLWQDLRATAERER
                     RSVFEVSSLLAVSAIGELPEKARVLSKAAAVVLRHGGAAVSNALLDHYRQSLQQMREV
                     GYLHYGVRQLTPYTRAAIGAFHPQRATLTDKLLDRL"
     gene            complement(3159..4394)
                     /locus_tag="VM692_13765"
     CDS             complement(3159..4394)
                     /locus_tag="VM692_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HUQ53288.1"
                     /translation="MEVTASPELAALATLAIVLGAGVVAASVAKRLGLPDIVLYLLAG
                     VLLGPVLGWLDVAPGSTLNQFILTAGAAYILFDGGTTLRFRVLQEIWLLLVVLATIGV
                     AITGAVVALAAHWLLGLPVLVAALLGATLASTDPATLVPVFRHVKVKERVAQLVMSES
                     AMNDATGAILTFTVLALLEAASRGGGVSLSGALVELLIAAGVGLVTGAACGYAAAALV
                     AGGRSPLPRELRGLITLLVVAGAYSLAVSFGGSGFMAVFTAGVMLMNYESFGHVLDGG
                     ERHQLELEIGNLSLLFRVLIFVLLGSQVDFALIERYLLPGMGIVAIFMLVARPATVFL
                     CALVDRRAGWTRDELLFMSWTRETGVIPAALVGLLAGRGAPNIDAIGAVTFIAILATI
                     LIQASTTRWWAARLGLLEP"
     gene            complement(4423..>4685)
                     /locus_tag="VM692_13770"
     CDS             complement(4423..>4685)
                     /locus_tag="VM692_13770"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HUQ53289.1"
                     /translation="EAAARFAFLLAVPVMTAAGLAEVAGYSGEPAAGPLDTRAIVIGL
                     VISAVTGFACIHYFMKWLTRFGMLPYVVYRLVLGAVLLWLLL"
BASE COUNT          836 a         1601 c         1539 g          709 t
ORIGIN      
        1 gaagtacaca cgccgcggag accttgctat gtcattcaga gaaaagagcg cgtggattac
       61 gctgatcaca ctgttactcg tctcggtcct ttggtcattg cattttccgt ggggtcggcc
      121 attcacgctg gcgccgggct ccgactcgtt cgttttccac gcgctggtgc tgtgcacgat
      181 cagcttcgtc gtcatcgtca tcgtcgcgca cgtcgtcgtg gccatccgcg cgccgcgtga
      241 agccagcgcg cgagccgacg agcgcgagca actgatcggg ctcaaagcga cgcggctcgc
      301 cgcgtacgtg tacgcggcgt tgagcatgag ctcggtgttt ctgattcacc tcggcgccaa
      361 tgagatcggc ttggcgtact tcctcgtcgc ctcgctggtg atcgccgaga gcatcagttg
      421 catcgcgcgc atcgtctatc accgccgagg cgtctgagcc atgagccaga cgacgatcag
      481 caacacgatc cgcaggttac ggttcgagcg caacgagatg acgcagcagg atcttgccga
      541 gcgcgtcggc gtcacgcgtc agacgatcaa tgcgatcgag gcgggcaagt actcgccgtc
      601 gctcgaggtc gcgtttcgca tcgccggcgc gctcggcgtg cggctcgaag acgtgttctc
      661 ctacgacgcc ggcgcacggc ccggagaaaa gccctgacgc cggccgagac gcagctcgtc
      721 acgcaaggtt ttgcgatctt gccgccacaa tggtcggcgg cgcgaatcgc gaccattgcg
      781 gcggcgcacg acgaggccgt cgcggcggcg gacccaagcg acgtcggagg aggcggcacg
      841 agcctccggg tctgggactt cgtcaatcga ggcgccgtct tcgacgagat ctatttgctc
      901 gtgcccgtgc tcgcggcctg ccggctcgtg atcggcgcgc agttcaagct cagcacgatg
      961 cattcgcgtg cgatcaaacc gcacgttcgt gccgacacgt tgcacgtaga ctttgcgcgc
     1021 gaagcgaccg acgcggcgcg cggcgcgtgg ccgatgctgg ggttcatcgt gatgctcgac
     1081 gatttcaccg cggcgaacgg cgcgacgcgg ttcgtgccgt gctcgcagca cgcggccgaa
     1141 cgcccgcctt gcggcgagcc cgtgcctgca tgcggtcccg cgggctcggt catcgtctac
     1201 aacggctccg tgtggcacgg gcacggcgcc aacacgaccg atcggccgcg ccgctccatt
     1261 caaggcgcgt acatccgccg cgacgcagtg ggattcgcaa tcgcggcgcg catgcgcccc
     1321 gagacactcg accgcatcgg gccgctcgcg agacaactgc tcgccgtttg aatcgcccgc
     1381 gcgacgaacg tagtgtacga aggcgatgac cgtgtacgtc tccatgctgc gcgccgtgaa
     1441 tgtcggcggc acgagccgca tcaagatgga cgcgctgcgc gccgtctacg aatcgatcgg
     1501 cctcaaggac gtgcgcacgt tgctgcagag cggcaacgtc gtgtttcgca gcgccattac
     1561 tgaccgcgcg cagctcgtga agcgcatcga gcaggagatc gagcggcagc tcgagctgca
     1621 agtcgacgtg atcgtgcgca cgctcgccga gatcgcgagc atcgtcgaac gcggccctgc
     1681 gctgtcgccg aaggccgata tggccaagtt gctcgtgatg tttttggccg gcgtccccga
     1741 cgccgcgggg caagcggcgc tcgtgcaatg gcacaaggag cgcaaagggc cggagatgct
     1801 cgagctgcgt ggccccgaga tctatcttta ttaccccgac ggcgtcggcc ggtcgaagct
     1861 gtcgggagcc gtcatcgaga acaagctcaa cacgtccggc acggcgcgca actggcacac
     1921 tctgcagaaa ctgctcgaga cgggacgcgc gctcgaagcg caggcctaga gacggtcgag
     1981 cagcttgtcc gtcagcgttg cacgctgggg atggaacgcg ccgatcgcgg cgcgcgtata
     2041 cggcgtgagt tggcgaacac cgtagtgcag atagcccacc tcgcgcattt gctgcagcga
     2101 ctgcctgtaa tgatcgagca gggcgttcga cacggcggcg ccgccatggc gcagcaccac
     2161 ggcagcggcc ttcgacaaca ctcgcgcttt ctcgggcagc tccccgatgg cggagaccgc
     2221 gagcagcgac gacacctcga acacggagcg tcgctcgcgc tccgccgtcg cgcgcaagtc
     2281 ctgccacagc cgcgtgaccg agctcggcga cggcagctgc ggcgccggca attgcttggc
     2341 gcccgctacg aacttggccc actcggcgcg taaccccttg acgtcgagcg gtggcgcgtt
     2401 gacgttctcc gccagttgcc cggagctctg ctcgagcccc gtaaggagct gctccatcgt
     2461 cgcgaacgag ccgtcttgcg cgagcaagcc ctccttcttg aggctgtccg cgatctcggg
     2521 gatcagctga cggccgaacc ccgcgacgtc ggcaagcgcc gcgagcaccc acactggtga
     2581 cgcgcgaaac gcgatgacgc ccatgagctc gatgccgttg ccgaccgagc gccgcagcag
     2641 aaagtcttcg ccgagcttct cgtcgctcgg tgtgccgcct tgcacgccgc cgacatcctc
     2701 gatcaggaac tgcagcgtca cgtcgacgag actgcggtag agccggcccc gccgaaaacc
     2761 gatcggcagc gcgacggccg ccacttcgcg gactgtgccc gctgcgatgg cggacgccga
     2821 gcgtacgagg cgctccggca gcgacacggc gtagcgaacg ccgcgatgcc ggccgagacc
     2881 tcgcagcttg tctttcacgc tcacggcgtg aaccgtatca cgacggcggc gggcggcact
     2941 cttgcgtttg gcccggcaaa tttgcgaatc ttacggccag gttcaacaac ccaaaggagg
     3001 tgatcccatg tctagtgaga ttcttggggt ttcagagcgt tgtgagtggt cctgccaagg
     3061 gcgaggcgct tagcaacgcg tacctttttg ggttcaagag accccatctc acggagggcc
     3121 gcgcaagcgg ccctctttgt tttttggagc cgtaacgcct acggctcgag cagcccgagc
     3181 ctggcggccc accagcgagt cgtcgacgcc tgaatcagga tcgtcgcgag gatcgcgatg
     3241 aacgtcacgg cgccgatcgc gtcgatgttc ggcgcgccgc ggcccgccag cagccccacg
     3301 agcgccgcgg ggatcacgcc ggtctcgcgg gtccacgaca tgaacaacag ctcgtcacgc
     3361 gtccaaccgg cgcgccggtc gacgagcgcg cagaggaaga ccgtcgcggg ccgggccacc
     3421 agcatgaaga tcgcgacgat gcccatgccg ggcagcagat accgctcgat cagcgcgaaa
     3481 tcgacttgac tcccgagtag cacgaaaatc aacacgcgaa acagcaacga caggttgccg
     3541 atctcgagct cgagctggtg gcgctcgccg ccgtcgagca cgtgaccgaa cgactcgtag
     3601 ttcatcagca tcacgccggc cgtgaacacg gccatgaagc cgctgccgcc gaaggagacg
     3661 gccaagctat aggctccggc gacgacgagc agcgtgatca ggccccggag ctcgcgcggc
     3721 agcggcgagc ggccgccggc tacgagcgcc gccgccgcat agccgcacgc cgcgccggtc
     3781 acgagaccga cgccggccgc aatcaacagc tccacgagcg cgccgctcag cgagacgccg
     3841 ccgccgcggc tcgccgcctc gagcagcgcg agcaccgtaa aggtcaggat cgcacccgtc
     3901 gcgtcgttca tcgccgattc gctcatgacg agctgcgcaa cgcgctcctt gaccttgacg
     3961 tgccggaaaa cgggcacgag cgtcgcgggg tcggtcgagg cgagcgtggc gccaagcagc
     4021 gcggccacga gcacgggtaa accgagcagc cagtgcgccg ccaacgccac gactgcgccc
     4081 gtgatcgcta cgccgatcgt cgcgagcacc acgagcagca gccaaatctc ttgcagcacg
     4141 cggaagcgca gcgtcgtgcc gccgtcgaac aggatgtacg ccgcccccgc ggtcaggatg
     4201 aattggttca gagtcgagcc cggagcgacg tcgagccacc cgagcaccgg cccgagcagc
     4261 acgccggcca gcagatacag gacgatgtcg gggaggccga gccgcttcgc gacgctcgcc
     4321 gcgacgacgc cggcgccgag cacgatcgcg agcgtcgcga gcgcggccag ctccgggctc
     4381 gccgtcactt ccatcgtctg cgccctccgc gcctccgcgg tatcacagca gcagccataa
     4441 cagcactgcg ccgagcacga ggcgatagac gacgtacggc agcatgccga aacgcgtgag
     4501 ccacttcatg aagtagtgga tgcatgcgaa acccgtgacg gccgagatca cgaggccgat
     4561 cacgatcgct cgcgtgtcga gcgggcctgc cgccggctcg ccgctgtagc ccgcgacctc
     4621 cgcgagcccg gccgcggtca tgacggggac cgccagcaga aacgcgaagc gtgctgcggc
     4681 ttccc
//
LOCUS       DATLEE010000629         3066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5958184, whole genome shotgun sequence.
ACCESSION   DATLEE010000629 DATLEE010000000
VERSION     DATLEE010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5958184"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="VM692_13775"
     CDS             complement(<1..1439)
                     /locus_tag="VM692_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HUQ53290.1"
                     /translation="MALVKKSKISATASKSTEPADLPTPAVKAPPARLAATKPQTVVE
                     RVAAAAEQLAAGLVEAAAATKELDLSMQQIAAGAEEAAGAAQEQSAAIKLIVSNLVTA
                     RGEVDQTARRADTLIATLAEATVQIAASVRAVERNAERQLASVKVIAELELRAKDISA
                     ITQTVGRISDQTNLLALNAAIEAARAGEQGRGFAVVADEVRALAETSDKNAQEVRDLA
                     AAMGDDVQRSVTALRTAAERAAKEAASAASVIEALQGRRDDVGAIAAASRQTLTSTVE
                     AERAATEVEAGANQIASAAEEQSAGAGEAQSAVRQQAKSLDQAQVSAQVLAKLAEQAR
                     RGKTGDDAGEQIGSAAEELSATIQQLSSAATEIMAAVEQIERASRLQAAATQQTSAAL
                     EQIEKTAKLAEANVRTADSRVREVDAALKTGRTAVDGLVSGMRANLADMRASVATVTQ
                     LGTLGRKVEKIVDAIALITVQTGMLAVSGS"
     gene            complement(1702..2622)
                     /locus_tag="VM692_13780"
     CDS             complement(1702..2622)
                     /locus_tag="VM692_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HUQ53291.1"
                     /translation="MTAARPPSATTKGRPLKVGLVQMAMGPDPAPNLAKAMDGVRDAA
                     KRGAQLIVLPELFRSRYFCQSEDAAQFGLAEAIPGPSTDALGTLAAELEVTLVASLFE
                     KRAAGLYHNTAAVLDGERGYLGKYRKMHIPDDPRYYEKYYFAPGDLGFKVFHTKPAEL
                     GVLVCWDQWYPEAARLTAMQGAELLVYPTAIGWHPEEKAEFGERQHGAWETVQRGHAV
                     ANGCFVVAVNRTGFEPDPLGKGGIEFWGQSFIAGTDGRIIVRAPVDREAVIVEELDLA
                     EIDAVRAGWPFFRDRRIDSYGDITKRFGND"
     gene            complement(2619..>3066)
                     /locus_tag="VM692_13785"
     CDS             complement(2619..>3066)
                     /locus_tag="VM692_13785"
                     /inference="COORDINATES: protein motif:HMM:NF037681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HUQ53292.1"
                     /translation="VDLTCDSDGKVTHYVSTSDNRFLPVHPTVNGTPYYLGFFLMGAY
                     EDIIGDAHNLFGRVTEAHIYADPDEDGDFWIEKIIHGTAVQDMLAQVQYFPNDLHRRM
                     SELVRAKIQQNIVRPTQGMEILDQYMACFPQTTYCDTRDIEREHQR"
BASE COUNT          432 a         1033 c         1034 g          567 t
ORIGIN      
        1 gagccgctga cggccagcat gccggtttgc accgtgatca aagcgatggc atcgacgatc
       61 ttttcgacct tccggccgag ggtgccgagc tgcgtgacgg tcgcgacgct cgcccgcata
      121 tccgcaagat tcgcgcgcat cccgctgacc aagccgtcga cggccgtccg gcccgtcttc
      181 agtgccgcgt cgacctcgcg tacgcgactg tctgcggtcc gaacgttcgc ctcggcgagc
      241 ttcgccgtct tttcgatctg ctccaaagcg gccgacgtct gctgcgtggc cgccgcttgc
      301 aggcgcgacg cgcgctcgat ctgctcgacg gccgccatga tctcagtggc cgcactcgag
      361 agctgttgaa tcgtcgcgga caattcctcg gcagccgagc cgatctgctc gccggcatcg
      421 tcgccggtct tgccgcggcg cgcttgctct gcaagcttgg cgagtacctg cgccgacact
      481 tgcgcctgat cgagcgactt ggcttgttgg cgcaccgccg attgcgcttc gccggcgccg
      541 gccgattgtt cctcggcggc gctcgcgatc tggttcgctc ctgcttcgac ttccgtggcc
      601 gcgcgctcgg cttcgacggt cgacgtcaaa gtctggcggc tggcggcggc gattgcgccg
      661 acgtcgtcgc gccgcccctg caaggcctcg atgacactcg cggccgaagc ggcctccttg
      721 gcggccctct cggccgcggt ccggagcgcc gtgacgctgc gctgcacgtc gtcgcccatc
      781 gcggccgcga ggtcgcggac ttcctgggca ttcttatccg acgtttcggc caaggcccgg
      841 acctcgtctg cgacgacggc gaacccgcgg ccctgctcac cggcgcgcgc ggcctcgatc
      901 gcggcattca gcgccagcag attggtctga tccgagattc ggcccacggt ctgcgtgatc
      961 gccgagatgt ccttggcgcg cagctcgagc tcggcgatga ctttcacgga tgcgagttgc
     1021 cgctcggcgt tgcgctcgac ggcgcgaacc gaggccgcga tctgaacggt ggcctccgcc
     1081 aacgtggcga tcaaggtgtc tgcgcggcgc gctgtctggt cgacctcgcc tcgcgccgtg
     1141 acgaggttac tgacgatgag cttgatggct gcggactgct cctgcgcggc ccccgcggct
     1201 tcctcggcgc ctgcggcaat ctgctgcatg gagaggtcaa gctctttggt cgcggccgcc
     1261 gcctcgacga ggccggccgc caattgctcg gcggcggcgg cgacgcgctc gacgaccgtt
     1321 tgcggcttgg tggcggccag acgcgcgggc ggggccttta cggcgggcgt cggcaaatcg
     1381 gcgggctcgg tggacttcga agcggtggct gaaatcttcg actttttgac tagtgccata
     1441 ggccgtcggg taccttcgac tttagagcgg gttgaaacgc gggacggcgt acggccggaa
     1501 ggccactgcg ccgctcgtta tttttggaaa gagtaacctt attttgtaac gaacagccgt
     1561 aagcctcatg cgggggcggc ctgttccaag ctcggagcct cgcacttgcc aatcgatgcg
     1621 tcttcgcagc gagagctatg caccgaacgt gcctggcggc tgagggatcg agcggcggac
     1681 ccgcaggagg cgacggcggc gtcagtcgtt gccgaagcgc ttcgtgatgt cgccgtagga
     1741 gtcgatgcgc cggtcgcgga aaaacggcca cccggcccgg accgcatcga tctcggcgag
     1801 gtcgagctcc tcgacgatga cggcctcgcg gtcgaccggt gcccgcacga tgatgcggcc
     1861 gtccgtgccg gcgatgaagc tctggcccca gaactcgatg ccgcccttgc ccagcgggtc
     1921 cggctcgaag cccgtgcggt tcacggcgac gacgaagcag ccgttcgcga cggcgtgacc
     1981 gcgttgcacg gtttcccacg cgccgtgttg ccgctcgccg aactcggcct tctcttcggg
     2041 atgccagccg atggctgtcg ggtagaccag cagctcggct ccttgcatcg cagtgagccg
     2101 cgcggcctcg ggataccact ggtcccagca gacgagcacg ccgagctcgg ccggcttggt
     2161 atggaacact ttgaaaccga ggtcgcccgg cgcgaagtaa tacttttcgt agtaccgcgg
     2221 atcgtcgggg atgtgcatct tgcggtactt gccgagatag ccgcgctcgc cgtcgagcac
     2281 ggcggccgtg ttgtgataga gccctgccgc gcgcttctcg aacaagctcg cgacgagcgt
     2341 gacctcgagc tccgccgcca gcgtgccgag cgcgtccgtg ctcggccccg gaatcgcctc
     2401 tgcgaggccg aactgcgccg cgtcctcgct ctggcaaaag tagcgcgagc gaaacagctc
     2461 cggcagcacg atgagctgcg cgccgcgctt cgcggcgtcg cgcacgccgt ccatggcctt
     2521 cgcgaggttc ggcgcggggt ccggccccat cgccatctgc acgagcccga ccttcagggg
     2581 gcgccccttg gtcgtcgccg aaggggggcg cgccgcggtc atcgctggtg ctcgcgctcg
     2641 atgtcgcgcg tgtcgcagta cgtcgtctgc gggaagcacg ccatgtactg gtcgaggatc
     2701 tccatgcctt gcgtcggccg cacgatgttc tgttgaatct tcgcgcgcac gagctcgctc
     2761 atgcgccggt gcaggtcgtt cgggaagtac tgcacctgcg ccagcatgtc ctgcacggcc
     2821 gtgccgtgga tgatcttctc gatccagaag tcgccgtcct cgtcggggtc ggcgtagatg
     2881 tgcgcctcgg tcacgcggcc gaacaggttg tgcgcgtcgc cgatgatgtc ctcgtacgcg
     2941 cccatcaaga agaagcccaa atagtacggc gtaccgttca cggtcggatg caccggcagg
     3001 aaacggttgt cgctcgtaga cacgtagtgc gtgaccttac cgtcggagtc gcaagtgaga
     3061 tcgacg
//
LOCUS       DATLEE010000630         2125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_5958242, whole genome shotgun sequence.
ACCESSION   DATLEE010000630 DATLEE010000000
VERSION     DATLEE010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5958242"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VM692_13790"
     CDS             <1..402
                     /locus_tag="VM692_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ53293.1"
                     /translation="NIVRAGRDYGWPEVSYGVQYDARQADGRKNVAVGTGATQMAGVE
                     QPIYYWVPSIAPSGMAFYTGNLFPEWRGNLFVGAMVGKHLVRLVLDGERVVAEEKLLE
                     ELDLRVREVRQGPDGALYVFGGDSLLKITPP"
     gene            479..772
                     /locus_tag="VM692_13795"
     CDS             479..772
                     /locus_tag="VM692_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HUQ53294.1"
                     /translation="MARKYSKKASKKVEKVMRERKRGTLRSGRSGKKVTSRKQAIAIG
                     LSEARREGDKVPARRKKRCFATRGAAKRRTSKRRTGRRSARRSTRRNTRKRAA"
     gene            829..1338
                     /locus_tag="VM692_13800"
     CDS             829..1338
                     /locus_tag="VM692_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53295.1"
                     /translation="MIEVLEALERNALSVWIREFPSVFGFPTILFLHTLGLAMVAGVS
                     IAIDLWILRGATLGAPLRIAGLARTMWLGFGINALSGVALLLAYPAKALTNWVFYLKM
                     ALVLLGVWLAARINRELEPATGARVTFDVGFDARRWATVSLLVWAAAIVTGRLLAYTH
                     SVLFASELP"
     gene            1335..1823
                     /locus_tag="VM692_13805"
     CDS             1335..1823
                     /locus_tag="VM692_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53296.1"
                     /translation="MTLPQLIYWLTHTPINHLVMDYKWTWPISESLHFCALALMVGTV
                     GTFDLRLLGLGKGIPPAVLHRSIRFGLVGFAVSAITGALFIFGQPDQYFYNNAFKVKV
                     VCLALLGLNVCAFYALEARQVLAIRAGEDASARAKVFAVISLTLLVAIMCAGRMLTFY
                     RP"
BASE COUNT          355 a          699 c          679 g          392 t
ORIGIN      
        1 aacatcgtgc gtgcgggccg cgactacggc tggcccgagg tctcgtacgg cgtgcagtac
       61 gacgcgcgtc aggccgacgg ccgcaagaac gtcgctgtcg gcacgggtgc gacgcagatg
      121 gcaggcgtcg agcaaccgat ctattactgg gtgccgtcga tcgcgccgtc gggcatggcg
      181 ttctacacgg gcaacctgtt ccctgagtgg cgcggcaatc tgttcgttgg cgcgatggtc
      241 ggcaagcatc tcgtgcgcct cgtgctcgac ggcgagcgcg tcgtcgccga ggagaagctg
      301 ctcgaagagc tcgacctacg cgtgcgcgaa gttcggcaag gaccggacgg cgcgctgtac
      361 gtgttcggcg gcgactcgct gctcaaaatc acgccgccgt agcggcgccg cccatagccg
      421 aagctggcac gcttcatgct gctctttccg cacgacacgg tagtcggacg gagcactcat
      481 ggcacggaag tacagcaaga aggcatcgaa gaaggtcgag aaggtgatgc gcgagcgcaa
      541 gcgcggcacg ttgcgcagcg gccgctcggg caagaaggtc acgagccgca agcaagcgat
      601 cgcgatcggc ctctccgaag cacgccggga aggcgacaag gttccggcgc gccgcaagaa
      661 gcggtgtttc gcgacgcgcg gagctgccaa gcgtcgcacg agcaagcgcc ggaccggccg
      721 ccgcagcgca cggcgcagca cgcgacgcaa cacgcgcaag cgagccgcct gatcacgcct
      781 ctgcgatgac gcagcggcgc agcgcggcgg tctgctacgc tgcgccgcat gatcgaggta
      841 ctcgaagcgc tcgaacgcaa cgcgttgagc gtatggattc gtgaattccc gtcggtcttc
      901 ggcttcccca cgattctctt cttgcacacg ttgggcctcg caatggtggc cggcgtcagc
      961 attgccatcg atctgtggat cctgcgcggc gcgacgttgg gtgcgccgct gcgcatcgca
     1021 ggccttgcgc ggacgatgtg gctcggcttc ggcatcaacg cactctccgg cgtcgctctg
     1081 ctgctggcct acccagccaa ggcgctgacg aattgggtct tctatttgaa gatggcgctc
     1141 gtgctgctcg gcgtctggct tgcggcccgc atcaatcgcg agctcgagcc ggccaccggc
     1201 gctcgcgtca cgttcgacgt tgggttcgac gcacggcgtt gggcgacggt gtcgttgctc
     1261 gtctgggcgg ccgcgatcgt cacgggccga ttgctggcct acacgcatag cgtgctgttc
     1321 gcgagcgagc tgccatgacg ctgccgcaac tcatctattg gctgacgcac acgccgatca
     1381 accacctcgt catggattac aagtggactt ggcccatcag cgaaagcctg cacttctgcg
     1441 cccttgcttt gatggtcggc accgtcggca cgttcgacct gcggctgctc ggtctcggca
     1501 agggcatccc gccggccgta ctgcatcgct cgatccgctt cgggctcgtc ggcttcgccg
     1561 tgtccgcgat caccggcgcg ttgttcatct tcgggcagcc ggaccagtac ttctataaca
     1621 acgcgttcaa ggtcaaagtc gtctgcctcg cgttgctcgg tctcaacgtc tgcgcgttct
     1681 acgcgctcga agcgcgccaa gtgctcgcga tccgcgccgg cgaggatgcg tcggcgcgcg
     1741 cgaaggtctt cgccgtcatc tcgctgacgt tgctcgtcgc catcatgtgc gccggccgca
     1801 tgctcacgtt ctaccgcccc tagcgcagcc ggcttatgtc gagcgcgttt acagatacgc
     1861 gccatgcatc gcgtgtagtt cgcgcacatc cgcgcgcaac tcgcgcatga ataaggacct
     1921 cgtgttttct ggcacacgtt ttggatcttg cgcccttcaa gcatccacac tgggcaaaag
     1981 cacgacatgg cgaacaacaa gagcaacgcg attcgtttgg caacgatcat cctcgctgcc
     2041 gctgcagccc atacggcttc ggcggtaccg atcgcgatca acggcttcgc cgtattccgc
     2101 gcggaccgaa ccgcgaatcc gatcg
//
LOCUS       DATLEE010000631         3330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3377303, whole genome shotgun sequence.
ACCESSION   DATLEE010000631 DATLEE010000000
VERSION     DATLEE010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3377303"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1741)
                     /locus_tag="VM692_13810"
     CDS             complement(137..1741)
                     /locus_tag="VM692_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53297.1"
                     /translation="MTVRIICICIATALPCGAIGGTKPMPASHFVTSAQCIACHSSLT
                     APSGEDVSIGFDWRATIMANSARDPYWHAAVRRETLDNPAAAAAIEDKCATCHMPMAR
                     FDARSSGAAGEVFANLDSAAPQHALAADGVSCTLCHQIAAAGLGEQASFDGGFEIEPR
                     ADGRVIFGPHDVDAGRRSVMRSAGTYAPSTGSHLQQSSLCATCHTLYTAALDESGRSI
                     GELPEQVPYKEWLHSDYRDSQSCQSCHMPEVAGAAPISSVLPQPREHVSQHTFLGGNA
                     FMLAMLNRYRDDLGVKAPARELDAAAEETKRFLGTRAAALAVTAAAQTSTGVDFTLEL
                     TSTTGHKLPTAYPARRAWLHVIVTDAAGRTVFESGAVRPDGSIVGNDNDAEAARFEPH
                     YESVTTPDEVQIYEAVMVDRDNAVTTALLRGVRFAKDNRLLPRGFDKGTAEPDVAVQG
                     AAFADADFTAGGDRVRYRIAVDANVARPFVVSAELLYQSIGYRWAANLRSYDAPETAR
                     FARYYADSAAGSAVRVASASATVAAR"
     gene            1820..2914
                     /locus_tag="VM692_13815"
     CDS             1820..2914
                     /locus_tag="VM692_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUQ53298.1"
                     /translation="MRIHYGIVLHATLALGMAAGARAQSPVPRDVYTRACAACHGADG
                     RGRGASEVGFDVPLPNFTDCDFAAREPDADWLAIAHRGGPVRGFDTLMPAFGEALTAD
                     ELAAAVAHIRTFCRDARWPRGELNLPRALFTEKAFPEDEAVITTTLVGEGLDSISHEF
                     VWEQRFGPVNQMELKLPITRADLGPAGWASGTGDLGIGVKHTLRHDLERGAILSVGGE
                     LVLPTGDETKGFGAGTTVLETSLLYGKALPRDSFLHVQGIVELPNDSALENELVVHAA
                     VGRTWTADAPFGRSWTPMLEVLAERDLESGAETEWDVAPQLQVSLSKRQHVLAAVGLR
                     QPITDRGNRPTQLVFYLMWDWYDGGVLEGW"
     gene            3011..>3330
                     /locus_tag="VM692_13820"
     CDS             3011..>3330
                     /locus_tag="VM692_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HUQ53299.1"
                     /translation="MNTRAHRIAARTATALSPRVWLALIVLAVGAGIAISRPAAAQQE
                     QDVTIVSADVLDDIVGPIALYPDDLVAIVLPASTYPLQIVQAARFLEDRKRDSSLQPD
                     EEWD"
BASE COUNT          520 a         1148 c         1132 g          530 t
ORIGIN      
        1 gaggccggcg cggcccctgt catgacaacg agcgcaacgg cgagaccgat gatgaaacgc
       61 acgattcctc cttgtgccaa tacgggccat cgcgccgttg gacggccgcg gcgcgccgga
      121 tcggtcccga cgagcatcag cgagccgcca cagtggcgct cgcgcttgcg acccgcacgg
      181 ccgagcccgc ggcgctgtcg gcgtaatagc gcgcgaatct cgccgtttcc ggcgcgtcgt
      241 aactgcgcag gttcgccgcc cagcgatagc cgatcgactg atacagcaac tctgctgaga
      301 ccacgaacgg gcgcgctacg ttcgcgtcga cggcgatgcg gtagcgcact cggtcgccgc
      361 cggccgtgaa gtcggcgtcg gcaaaggcgg cgccttgcac ggcgacgtcg ggctccgcgg
      421 tgcccttgtc gaacccacgc ggcagcagcc ggttatcctt cgcgaagcga acgccgcgca
      481 gcagcgccgt ggtcacggcg ttgtcgcggt cgaccatgac cgcttcgtaa atctgcacct
      541 cgtccggcgt cgtgacggac tcgtaatgcg gctcgaagcg cgcggcctcc gcatcgttgt
      601 cgttgccgac gatgctgccg tcgggccgca cggcgcccga ctcgaacacg gtgcggcccg
      661 ccgcatcggt cacgatgacg tggagccagg cgcgacgcgc gggataggcg gtcggcagct
      721 tgtgacccgt cgtgctcgtc agctcgagcg tgaaatcgac acccgtgctc gtctgcgccg
      781 cggccgtgac ggcaagcgcc gccgcgcgcg tgcccaagaa gcgcttcgtc tcctcggccg
      841 ccgcatcgag ctcccgcgca ggcgccttga cgcccaagtc gtcgcgataa cgattcagca
      901 tggcgagcat gaaggcgttg ccgccgagaa acgtgtgctg cgatacatgc tcgcgcggct
      961 gcggcagcac ggacgagatc ggcgctgcac cggcgacttc cggcatgtgg cacgactggc
     1021 aactctggct gtctcggtag tcgctgtgca gccactcctt atagggcacc tgctccggca
     1081 gctcgccgat gctccggccc gactcgtcga gcgctgcggt gtagagcgtg tggcaggttg
     1141 cgcagagcga cgattgctgc aagtgactgc ccgtgctcgg cgcgtacgtg ccggccgaac
     1201 gcatcacgga ccggcgtccc gcatcgacgt cgtgcgggcc gaagatgacg cggccgtccg
     1261 cgcgcggctc gatctcgaac ccgccgtcga agctcgcctg ctcgccgagc ccggcggccg
     1321 cgatctgatg gcagagcgtg cacgacacgc cgtcggcggc cagcgcgtgc tgcggcgccg
     1381 cgctgtcgag attcgcgaac acctcgcccg ccgcgcccga gcttcgggcg tcgaagcgag
     1441 ccatcggcat gtggcaggtc gcgcacttgt cctcgatggc cgccgccgcc gcggggttgt
     1501 cgagcgtctc gcggcgcact gcggcgtgcc aatacgggtc gcgcgccgag ttcgccatga
     1561 tcgtcgcgcg ccagtcgaag ccaatcgaga cgtcttcgcc tgacggcgct gtcagcgagc
     1621 tatgacaggc gatgcactgc gccgaggtga cgaagtgact cgccggcatc ggcttcgtgc
     1681 cgccgatcgc gccgcacggc agagcggtcg cgatgcaaat gcaaatgatt cgcacagtca
     1741 tgattttgct gatcttttga gcggcggtcg gcgtcactat actgcggcgc atcgtttgca
     1801 caacgctcga gagcagcgct tgcgtattca ctacggcatc gttttgcacg ccacgcttgc
     1861 gctcgggatg gcggccggcg cccgcgcgca gtcgcccgta ccgcgcgacg tctacacgcg
     1921 agcttgcgcg gcgtgtcatg gcgccgacgg tcgcggccgc ggcgcgagcg aggtcggctt
     1981 cgacgtgccg ttgccgaact tcacggactg cgacttcgcc gcgcgcgagc cggacgcgga
     2041 ttggctcgcg atcgcgcatc gcggcggccc cgtgcgcggc ttcgatacgc tgatgccggc
     2101 gttcggcgaa gcgttgaccg ccgacgagct cgccgccgcc gtcgcgcata tccgcacgtt
     2161 ctgccgcgac gcccgctggc cgcgcggcga gctcaatctg ccgcgcgcgt tgtttactga
     2221 aaaagcgttt cccgaagacg aagccgtcat cacgacgacg ctcgtcggcg aagggctcga
     2281 ctcgatcagt cacgagttcg tctgggaaca gcgcttcggc cccgtgaatc agatggagct
     2341 caagttgccg atcacgcgcg cggatctcgg ccctgccggc tgggcgagcg gcaccggcga
     2401 tctcggcatc ggcgtcaagc acacgctgcg ccacgacctc gagcgcggtg cgatcctgag
     2461 cgtcggcggc gagctcgtgc tgccgacggg cgacgagacc aaaggcttcg gggccggcac
     2521 gaccgtgctc gaaacgtccc tgctctacgg caaggcgctg ccgcgcgact cgttcctcca
     2581 cgtgcaaggc atcgtcgagc tcccgaacga ctccgcgctc gagaacgagc tcgtcgtcca
     2641 tgcggccgtc ggtagaacct ggacggccga cgcgccgttc ggccgcagct ggacaccgat
     2701 gctcgaggtg ctcgcggagc gcgacctcga atcgggcgcc gagaccgagt gggacgtggc
     2761 gccgcagctg caagtttcct tgagcaagcg ccagcacgtc ctcgcggccg tcggcctgcg
     2821 gcagcccatc acggaccgcg ggaaccggcc gacgcagctc gtgttctatt taatgtggga
     2881 ttggtacgac ggcggcgtcc tggaaggctg gtagtaggga atcggccatt aaggcgccgt
     2941 tcagctgccg tccgctaggg taaacgccat gctggggcgc aaagcgcccg caggtgaacg
     3001 gagaccatcg atgaacacga gagctcacag aatcgctgcc cgaacggcca cggcgctgtc
     3061 gccgcgcgtt tggttggccc tcatagtgct cgcggtcggc gcggggatcg caatctcgcg
     3121 tcccgccgcg gcgcaacaag agcaggacgt cacgatcgtc tccgccgatg tgctcgacga
     3181 catcgtgggc ccgattgcgc tctaccccga cgacctcgtc gcgatcgtgc tgccggcctc
     3241 gacctatccg ctgcagatcg ttcaagccgc gcgcttcctc gaggaccgta aacgcgattc
     3301 gagcctccag ccagacgagg agtgggacga
//
LOCUS       DATLEE010000632         2762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3379126, whole genome shotgun sequence.
ACCESSION   DATLEE010000632 DATLEE010000000
VERSION     DATLEE010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3379126"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VM692_13825"
     CDS             <1..880
                     /locus_tag="VM692_13825"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53300.1"
                     /translation="WRNPHVYFELEVRDSDGKANVQQIEAGPASSFVAMGFNADAIRK
                     GDRLKVQAKPNRAGPGRVVLGWQLTKVDGTVIPLHVRAVTTSVAPTAEATGLAGVWLP
                     QATGFSSLAVAARDWPLTDKARALAAATRDARAAQRAACVPYGPPALMAVPSAATVEV
                     GTSEVTFTIDAMNVRRVVHLDQPSHPVPLEPTVLGHSIGHWEGGTLVVDTAGFTAHPE
                     GYAFDLPSSAAKHVVERFSLSADRKHLEYEATVDDSEYFTAPVKHRSQWDYRPELKPS
                     NLPCDPEAASRFTTDE"
     gene            complement(889..1350)
                     /locus_tag="VM692_13830"
     CDS             complement(889..1350)
                     /locus_tag="VM692_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018977795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4385 domain-containing protein"
                     /protein_id="HUQ53301.1"
                     /translation="MKEFDYTLDYANLDLRQRPELYRIGRGEQGVLLVEPYKSEILPH
                     WRFKTATEARRSAAAIYRLYRRYLRARDFVGMDMARKFLQMGFTRARRYANHRGGRKY
                     ARGTKRLLPRSKPDPEKAAAAEIFRQQWERVRNDAAYLAARAAHRKRFEQP"
     gene            1471..2199
                     /locus_tag="VM692_13835"
     CDS             1471..2199
                     /locus_tag="VM692_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018073340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HUQ53302.1"
                     /translation="MIDLYYWPTPNGKKVAILLEETQLPYRVVPVDIGKGDQFTESYL
                     ALNPNHRMPVIVDREPLGGGKPISVFESGAILMYIAEKAGRFWPQTPREKYEVAQWVF
                     WQMANQGPKFGESGHFRRAAQKPENGDLKYPLRRFDDEVNRLLGVMNNRLYDRRYLGG
                     NDYSIADIVSYPWSLNWQTLGQNIDEFKHVKRWLADVGGRPAVRRGMEVGKELAVDFS
                     KLSKEELDRRMKILSNQRALPAPG"
     gene            complement(2131..>2762)
                     /locus_tag="VM692_13840"
     CDS             complement(2131..>2762)
                     /locus_tag="VM692_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53303.1"
                     /translation="AASRIGATRSIACTSAIQLDLGRVTRRPLDRALMLRKGIDDIRL
                     LRSADPRIAAQMLDLDAYRPVSSQPAIQRDMSIAVATEITPEVLADRVRDVVGARADA
                     LESVEVISQTAYSELPSAARDRLGIAAAQKNVLLRLVIRDLTRTLTSEEANAAARRGV
                     RRAAPGLGEDVGPRRLNSLQAQRPAGALTGRRQRALVGQDLHAAIELFL"
BASE COUNT          481 a          882 c          920 g          479 t
ORIGIN      
        1 gtggcgcaat ccgcacgtct acttcgagct cgaggtccgc gattcggacg gcaaagcgaa
       61 cgtgcagcag atcgaggcgg ggccggcctc gtcgttcgtt gcgatgggct tcaacgcgga
      121 cgcgatccgc aagggtgacc gcctgaaggt tcaggccaag ccgaaccgcg ccgggcccgg
      181 ccgtgtcgtg ctcggttggc aattgacgaa ggtcgacggg acggtaattc cgttgcacgt
      241 gcgcgccgtg acgacgagcg tcgcacccac cgcggaggcg acgggcctcg ccggagtatg
      301 gctcccgcaa gcaaccggct tctcgagctt ggccgtcgcg gcccgcgatt ggcccttgac
      361 cgacaaagca cgcgcgctag ccgcggccac gcgcgatgcg cgcgccgccc aacgcgccgc
      421 gtgcgtgccg tacggtccgc cggcgctgat ggccgtgccg tcggccgcga cagtcgaagt
      481 cggaacgtcc gaggtcacgt tcacgatcga cgcgatgaac gtgcggcgcg tcgtgcatct
      541 cgatcaaccg agtcatccgg ttccgctcga gccgacggtg ctcgggcatt cgatcggtca
      601 ttgggagggc ggcacgctcg tcgtcgatac ggccggtttc acggcgcatc ccgagggtta
      661 cgcgttcgac ctgccgtcga gcgcagccaa gcacgtcgtc gagcggttct ctctgagcgc
      721 ggaccgcaag cacctcgagt acgaggcaac agtcgacgac tccgagtatt tcactgcgcc
      781 cgtcaagcac cgttcgcaat gggactatcg gccggagctg aagccgtcga atctgccgtg
      841 cgatcccgag gccgcgagtc gcttcacgac cgacgagtga tgctgcagct atggctgctc
      901 gaatcgcttg cgatgcgcag cacgcgctgc aaggtacgcg gcatcgttgc gcacgcgctc
      961 ccactgctgg cggaagattt cggcggcggc cgccttctct gggtcgggct tcgatcgcgg
     1021 caataaacgc ttcgtgccgc gcgcgtattt acgcccgccg cggtggttcg cgtaacggcg
     1081 tgcgcgcgtg aagcccatct gcaggaactt gcgcgccatg tccatgccga cgaaatcgcg
     1141 cgcgcgcagg taacggcgat agagccggta gatcgcagcg gccgatctgc gggcttcggt
     1201 ggccgtcttg aagcgccagt gcggcaggat ttcgctcttg tacggctcga cgagcaggac
     1261 gccttgctcg ccgcgcccga tgcggtagag ctccggccgt tgacgaaggt cgagattcgc
     1321 gtagtccagg gtgtagtcga actctttcat ctgtcatttg cgagttgcgc ctgagcgcag
     1381 ctgccgagtt gcagtttgtg tgccacgccc gggacgagat tagactctgg tcatggtctc
     1441 gagcagagag tcggcaccga ggagacgcct gtgatcgatc tttactattg gcccacgccg
     1501 aacggcaaga aagtcgcgat cttgctcgag gaaacgcagc tgccgtatcg agtcgtgccc
     1561 gtcgatattg gcaagggcga tcagttcacc gagagctatc tcgcactgaa cccgaaccat
     1621 cgcatgcccg tcatcgtgga ccgcgagccg ctgggcggcg gcaagccgat ctcggtgttc
     1681 gaatccggcg cgatcctgat gtacatcgcc gagaaggccg gacgcttttg gccgcagacg
     1741 ccgcgcgaaa aatacgaagt cgcgcaatgg gtgttttggc agatggccaa ccaaggcccg
     1801 aagttcggcg agtccggtca cttccggcgt gccgcgcaga aacccgagaa cggcgacctc
     1861 aagtatccgc tgcgacgttt cgacgacgag gtcaatcgtc tgctcggcgt catgaacaac
     1921 cggctgtacg accggcgtta cctcggcggc aacgactact cgatcgccga catcgtgagc
     1981 tatccgtggt cgttgaattg gcaaacgctg ggtcagaaca tcgacgagtt caagcacgtc
     2041 aaacgctggc tggccgacgt cggcggccgc ccggccgtgc ggcgcggcat ggaagtcggc
     2101 aaggagcttg ccgtcgactt ctcgaagctg tcaaaggaag agctcgatcg ccgcatgaag
     2161 atcctgtcca accagcgcgc gttgccggcg cccggttagc gcgccagccg ggcgttgcgc
     2221 ctgcaacgaa ttcagccttc gcgggcccac gtcttcaccg agccccggtg cagcgcggcg
     2281 tacacctcgt cgcgcagcgg cgttcgcttc ctcggacgtc agggttcgcg tgaggtcgcg
     2341 aatgactagc cgcaacagca cgttcttctg agccgcagcg ataccgagtc ggtcccgcgc
     2401 ggcgctcggt agctcggagt aggcggtctg cgatatcacc tcgaccgatt cgagcgcgtc
     2461 ggcacgcgca ccgacgacat cgcgcacgcg atctgcgagc acttccggcg tgatctcagt
     2521 ggccactgca atcgacatgt cacgctggat ggcgggttgc gacgacacgg gccgataagc
     2581 gtcgaggtcg agcatctgcg ctgcgatgcg cggatcggcg gaacgcagca gccgtatgtc
     2641 gtcgatgcct ttgcgcagca tcagcgcgcg gtcgagcggc cgccgcgtca cgcgcccgag
     2701 atcgagctgg atcgccgacg tgcaggcgat cgatcgagtc gcgccgatac gtgaggccgc
     2761 ga
//
LOCUS       DATLEE010000633         4016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7099044, whole genome shotgun sequence.
ACCESSION   DATLEE010000633 DATLEE010000000
VERSION     DATLEE010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7099044"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VM692_13845"
     CDS             complement(<1..242)
                     /locus_tag="VM692_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53304.1"
                     /translation="MIPWRPVCVFVAIAVGATTGIAVLCAALAWSVSSPEWAMVAPFA
                     MWAPAFARFVTRSTVDRSFESTLPLNRWGDGGALVIL"
     gene            complement(247..1017)
                     /locus_tag="VM692_13850"
     CDS             complement(247..1017)
                     /locus_tag="VM692_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53305.1"
                     /translation="MRVAVVIAAAVVAAAQASAQPARDLSGHWDRSSGIESFANTPGG
                     TRDNAQPGAREAPFTPAGRAAFDANKPGYGPRRAMARNDPMGRCEPLGLVRNLVTEIV
                     APHSTFELVQMPTRIVQFFEYRHDWREIWLDGRALPEGDDLYPKWNGFSVGRWQGDEL
                     VVESVGFDARTWLDKLGYPHSENMRVEERYRRVDGETLELVITVTDPEYYTEPWRSDV
                     KRFRANAAKAKRWDEQIHCVPAEEMTYQDLVGTGNRID"
     gene            complement(1019..1408)
                     /locus_tag="VM692_13855"
     CDS             complement(1019..1408)
                     /locus_tag="VM692_13855"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53306.1"
                     /translation="MLRSKKWRRAYAATAALGLLGLVPAAAHHGGDVEWRNQAVGPIT
                     GTATKFAFQFPHVFFEMNVEDKGVTSAWTITTRWTPTILRDHGWTRESIKPGDKITVT
                     YLPHVEKQHIGQMQTIDVNGKSLPLSF"
     gene            1542..3488
                     /locus_tag="VM692_13860"
     CDS             1542..3488
                     /locus_tag="VM692_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUQ53307.1"
                     /translation="MTQLNTPYPATAYLTGFLRAHGAELDLTVSQADASLLLFLRLFS
                     ARRVAALAETLEGRARAARRTPPMPPSIAHFVAQAARYVATVEPAIRFLQGRDPSLAL
                     RIVGREFLPEGPRFAPLASTPEDPLAWAFGSLGTTDQARYLASLFVDDLADVWRLGLD
                     PKFELARYGEKLAASAPTFEPLAAALDSDATLVDEALAEITRQLVATHRPDVVGMSAP
                     FPGNVYGAFRMAQTIRAAAPAAKLVLGGGWVNTELRVLREPRVFDYFDYVTLDDGERP
                     LLNLLARFAGKQAPLVRTYVRKGDTVVLETNAAQHDFLQRDTGIPTYDGLPLADYVSV
                     MEMLNPMHRLWSDGRWNKITLAHGCYWKKCSFCDVSLDYIGRYDRPSADIVLERIRAL
                     IAETSQTGFHLVDEAAPPAGMRALAKRLLAENLSITWWGNIRFEKTFTPELCGLLAEA
                     GCVAVSGGLEVASDRLLGLMKKGVTVEQVARVTRAFTDAGIMVHAYLMYGFPSETVQE
                     TVDALERVRQLFEAGCIQSAYWHRFSATAHSPVGLEPEQYGITLHTPPAITFAHNDLA
                     FDDPVGADHDLLGLGLRKALYNYMLGIGLDADVRTWFEAPRRAERPARGRRMRRRAAG
                     APLRGLHVPATTVAPDLIAGYLAR"
     gene            3489..>4016
                     /locus_tag="VM692_13865"
     CDS             3489..>4016
                     /locus_tag="VM692_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HUQ53308.1"
                     /translation="MAVLIALNKPYGVVCKFSPEPSKRTPLGSPRRGTLADYVAVRDV
                     YPAGRLDTDSEGLLLLTDDGALQARIANPRHKLAKIYWAQVEGVPTEEALATLRRGVD
                     LGDFTTKPAGARLIDEPQNLWPRDPPIRYRAKIPTTWLELTLREGKNRQVRRMTARVG
                     FPTLRLVRAAIGRIDL"
BASE COUNT          639 a         1427 c         1304 g          646 t
ORIGIN      
        1 aggatcacga gcgcgccgcc gtcgccccaa cgattcagag gcagtgtcga ctcgaagctc
       61 cgatccacgg tgctgcgggt gacgaagcga gcgaacgccg gcgcccacat cgcgaacggc
      121 gccaccatcg cccattccgg cgagctcacg gaccacgcca gtgccgcgca gagaacggcg
      181 atgcccgtcg tcgcgccgac cgcgatcgcg acgaacacgc atacggggcg ccaaggaatc
      241 atcgcgtcag tcgatgcggt tgccggtgcc gacgaggtcc tggtacgtca tttcctcggc
      301 cgggacgcaa tggatctgct catcccagcg cttcgccttc gcggcgttcg cccgaaaacg
      361 cttcacgtcg ctgcgccagg gctccgtgta gtactcgggg tccgtgacgg tgatgacgag
      421 ctcgagcgtt tcgccgtcga cgcgtcggta gcgttcctcg acgcgcatgt tctccgaatg
      481 cggatagcca agcttgtcga gccacgtccg cgcgtcgaag ccgaccgatt cgacgacgag
      541 ctcgtcgccc tgccagcggc cgacggagaa gccgttccac ttcggataca agtcatcgcc
      601 ttcgggcagc gcgcggccgt cgagccagat ctcgcgccaa tcatggcggt actcgaagaa
      661 ctgcacgatg cgcgtcggca tttgcacgag ctcgaacgtc gaatgtggcg cgacgatctc
      721 ggtgacgagg ttgcgcacga gacccagcgg ctcgcagcgg cccatcgggt cgttgcgcgc
      781 catcgcgcgg cgcgggccgt agcccggctt gttggcgtcg aacgcggcgc gaccggcagg
      841 ggtgaacggc gcttcacgag cacccggctg cgcgttgtcg cgcgtgccgc ccggcgtgtt
      901 cgcgaacgac tcgatgccgg agctgcgatc ccaatgaccc gacagatcgc gcgcaggctg
      961 cgcgcttgcc tgcgctgccg cgacgacggc ggccgcgatg acgacggcga cgcgcatgtc
     1021 agaacgacaa cggcaacgac ttgccgttga cgtcgatcgt ctgcatctgg ccgatgtgct
     1081 gcttctcgac gtgaggcagg tacgtgacgg tgatcttgtc acccggcttg atcgactcgc
     1141 gcgtccaacc gtgatcgcgc aagatcgtcg gcgtccaccg cgtcgtgatc gtccacgcgc
     1201 tcgtcacacc tttgtcctcg acgttcatct cgaagaacac gtgcgggaac tggaacgcga
     1261 acttcgtcgc ggtgcccgtg atcgggccga cggcctggtt gcgccattcg acgtcgccgc
     1321 cgtgatgcgc ggcggccggc acgagcccga gcaagcccag agccgccgtc gcggcatacg
     1381 cacggcgcca cttcttcgat ctgagcatga tcaccccctc gcttgcagag gcccacgtta
     1441 cgaccaaagg tcggcggttt tcaaacgcct ggccgatgac cgccacagcc caactgggct
     1501 aacctcgtcg gcccatgcgc gtcttgctcg ttacaccgcc gatgacccag ctcaatacgc
     1561 cgtaccctgc gacggcctat ttgacggggt ttctgcgtgc gcacggtgcg gagctcgacc
     1621 tgacggtctc gcaagccgat gcgtcgctgc ttctgttcct gcggctgttc tccgcacgcc
     1681 gcgtcgcggc gctcgccgag acgctcgaag gccgcgcccg cgccgcccgc cgcacaccgc
     1741 cgatgccgcc gtcgatcgcg cacttcgtcg cgcaggcggc gcgctacgtc gcgaccgtcg
     1801 agccggcgat ccgcttcctg caaggccgcg atccgagcct cgcgctgcga atcgtcggcc
     1861 gcgagttcct gcccgagggt ccgcgcttcg cgccgctcgc gagcacgccg gaagacccgc
     1921 tcgcgtgggc cttcggcagc ctcggcacga cggatcaggc gcgctatctc gcgagcctgt
     1981 tcgtcgacga cctcgccgac gtctggcgct tggggttaga tcccaagttc gagctcgcgc
     2041 gctacggcga gaagctcgcc gcgagcgcgc cgacgttcga gccgctagct gccgcgctcg
     2101 acagcgacgc aaccctcgtc gacgaagcgc tcgccgagat cacgcgccag ctcgtcgcca
     2161 ctcaccggcc cgacgtcgtc gggatgtcgg caccgttccc cggcaacgtc tacggcgcgt
     2221 tccgcatggc gcaaacgatc cgtgccgcag cgcccgccgc gaagctcgtg cttggcggtg
     2281 gctgggtcaa cacggagctg cgcgtgctgc gcgagccgcg cgtgttcgac tatttcgact
     2341 atgtgacgct cgacgacggc gagcggccgc tcctgaacct gctcgcgcgc ttcgccggta
     2401 agcaagcgcc gctcgtgcgc acttacgtgc gcaagggcga caccgtcgtg ctcgagacga
     2461 acgcggcgca gcacgatttc ctgcagcgcg acaccggcat cccgacctac gacggcttgc
     2521 cgctcgccga ctacgtctcg gtcatggaga tgctgaatcc gatgcaccgg ctctggtccg
     2581 acggccgctg gaacaagatc acgctggcgc acggctgcta ctggaagaag tgcagcttct
     2641 gcgacgtgtc gctcgactac atcgggcgtt acgaccggcc gagcgccgac atcgtgctcg
     2701 agcgtattcg cgcgctgatc gcggagacga gccagaccgg ctttcacctc gtcgacgagg
     2761 ccgcgccgcc ggccggcatg cgcgcgctcg cgaagcggct gctcgccgag aatctcagca
     2821 ttacgtggtg gggcaacatc cgcttcgaga agacctttac gccggagctc tgcggtttgc
     2881 tcgccgaagc cggctgcgtc gccgtcagcg gcggcctcga ggtcgcgtct gatcgactgc
     2941 tcgggctcat gaagaaaggc gtcacagtcg agcaagtcgc gcgcgtcacg cgcgcgttca
     3001 cggacgccgg catcatggtg cacgcctatt tgatgtacgg ctttcccagc gagaccgtgc
     3061 aagagaccgt cgacgctctc gagcgcgtgc gtcagctgtt cgaagccggc tgcatccagt
     3121 ccgcgtactg gcaccggttc agcgcgacgg cgcactcgcc agtcggcttg gaacccgagc
     3181 agtacggcat cacgctgcac acgccgccgg cgattacctt cgcgcacaac gacctcgcgt
     3241 tcgacgatcc cgtcggcgcc gatcatgacc tcttgggatt gggcctgcgc aaagcgctct
     3301 acaactacat gctgggtatc ggcctcgatg ccgacgtgcg gacgtggttc gaggcgccga
     3361 gacgggccga gcgcccggcg cgcggccggc gcatgcgtcg ccgcgccgcc ggggctccgc
     3421 tccgcgggct acacgtgccg gcgacgacgg tcgcacccga cctgatcgcc ggctatctcg
     3481 cgcggtagct ggcagtgctg atcgcgctca acaagccgta cggcgtcgtc tgcaaattca
     3541 gcccagagcc cagcaagcgc acgccccttg gtagtccccg aagggggacg ctggccgact
     3601 atgtcgcggt gcgcgacgtc tacccggccg gtcggctcga taccgatagc gagggattgc
     3661 tgctgctcac ggacgacggc gcgctgcagg cgcgaatcgc gaacccgcgc cacaagctcg
     3721 cgaaaatcta ctgggcgcaa gtcgaaggcg tgccgaccga ggaagcgctg gccacgctgc
     3781 gccgcggcgt cgatctcggc gacttcacga cgaagcccgc cggcgcgcgt ctcatcgacg
     3841 agccgcaaaa tctttggccg cgcgacccgc cgatccgcta ccgcgcgaag atccctacga
     3901 catggctcga gctgacgctg cgcgaaggca agaaccgcca ggtccgccgc atgacggccc
     3961 gcgtgggctt cccgaccttg cgcctcgtgc gtgccgccat cggccgcatc gatctc
//
LOCUS       DATLEE010000634         2389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7828875, whole genome shotgun sequence.
ACCESSION   DATLEE010000634 DATLEE010000000
VERSION     DATLEE010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7828875"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VM692_13870"
     CDS             complement(<1..189)
                     /locus_tag="VM692_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HUQ53309.1"
                     /translation="MAELSEKQRDNLDDDQFAFPKERKEPLNNAGHVRNAIARFNQVE
                     GVSDEERDAAWRRIKKAAD"
     gene            complement(449..1000)
                     /locus_tag="VM692_13875"
     CDS             complement(449..1000)
                     /locus_tag="VM692_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011750394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HUQ53310.1"
                     /translation="MTILHIDSSISGDQSASRTLTRSIVDQLTATQPGQRVIYRDLAA
                     DPLPHLTLDAFADTSVLDEFLSADTVVVGAPMYNFTLPTQLKAWIDRLAIAGTTFRYT
                     ENGPEGLVKGKRVIIALARGGFYGEGTPAAALEHLETYLRGVFNFIGVEPEFVAADGL
                     AIGPAQREESISQALGETLRLAA"
     gene            1147..1524
                     /locus_tag="VM692_13880"
     CDS             1147..1524
                     /locus_tag="VM692_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUQ53311.1"
                     /translation="MKDPSNPVCRTISTLLSRIGDKWTVLVVTSLGSGSRRFNELRRE
                     IPSVSQRMLTLTLRNLERDGLVRRTVTPTIPPRVDYELTEMGHSLLKPVTALSNWALN
                     HVDEIHGAQSRFDAEHDARSEAA"
     gene            1521..>2389
                     /locus_tag="VM692_13885"
     CDS             1521..>2389
                     /locus_tag="VM692_13885"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HUQ53312.1"
                     /translation="MSAEQPGTNLAALLDRADEVATRGDARAAVSFYQAALLQARSAR
                     GLDAGMVLRLRRGQEFIQLRTKEFERTLDQSLPTDPPADAAAALRLNHAIDIVKGTRE
                     LYPQQPSMFYYPYLPQRQFFERQEFAWTVALEAATREIVHELRALLSEGADFRPYVER
                     DPERPPRDFHGLTENRSWTALYIWKHGRFVDEIAERCPATVRALEGVPLSRIGSRTPS
                     VFFSRLEPGAHIPPHTGMLNSRLICHLPLIVPAGCTLRVGNETRAWEEGELLIFDDSI
                     EHEARNPTGEVRIV"
BASE COUNT          426 a          738 c          775 g          450 t
ORIGIN      
        1 gtcggcggcc ttcttgatcc gccgccacgc cgcatcgcgc tcctcgtcgc tgacgccttc
       61 aacctggttg aaccgggcga tcgcattgcg cacgtggccg gcgttattga gcggctcctt
      121 gcgctccttg ggaaacgcga attggtcgtc gtccagattg tcgcgctgct tctcgctgag
      181 ttcggccatg gctgctcctt tcgaccagca aaacgcgcaa gccgccatgt cgttcgcgtg
      241 ggcgataaaa agccccggcg ccccacggga ggggagaggg cgccggggct ggatgcgacg
      301 gccggtgcat gaaccggttt agcaggatgg ggcaatcctg tgtcgtcgct gatcatctat
      361 cgtcgcatgg agcgtcttta gccgcccagt cttgcctcga tccgttcgcg ggatgacatt
      421 tgcgtcatga aactcgttgg ggggttgctc aggccgccag gcgcagcgtt tcgcccagtg
      481 cctggctgat cgactcctcc cgctgcgcgg gcccgatggc gaggccgtcg gcggcaacga
      541 actccggctc gacaccgatg aagttgaaca cgccgcggag gtaggtctcg agatgctcga
      601 gcgcggccgc gggcgtgcct tcaccgtaga agccgccgcg ggcgagcgcg atgatcacgc
      661 gcttgccctt gaccaaccct tccgggccgt tctcggtgta gcggaatgtc gtcccggcga
      721 tcgcgagccg gtcgatccag gccttgagct gcgtcggcag cgtgaagttg tacatcggcg
      781 cgccgacaac caccgtgtcg gcactgagga actcgtcgag caccgacgtg tcggcgaagg
      841 cgtcgagcgt cagatgcggc agtggatcgg cggcaaggtc gcgatagata acgcgctgcc
      901 cgggctgcgt agccgtgagc tggtcgacga tcgagcgcgt aagcgtccgc gacgcgctct
      961 ggtcgccgct gatgctactg tcgatatgaa ggatagtcac taatgcctcc ttggtattga
     1021 ttcgtaaccg aggcacgcta gataactacc ctcgaatcgc cggcaagaac gcactttttt
     1081 gtgggtgacg aacaagtttg tgcgcgggaa catggctgtg gccggtcgcg agaaacggag
     1141 agaatcatga aggatccaag caatccggta tgccgcacga tcagcacctt gctgtcgcgg
     1201 atcggcgaca aatggacggt gctagtcgtc acctcgctgg gcagcggttc gcggcgcttc
     1261 aacgaattga ggcgcgagat cccgagcgtg tcgcaacgga tgctgacgct gacgctacgc
     1321 aacctcgagc gcgacgggct ggtccgccgc accgtgaccc caacgatccc gccgcgagtc
     1381 gactatgagc tgaccgagat gggccactca ctgctcaagc cggtcaccgc cttgtcgaat
     1441 tgggcgctca atcacgtcga cgagattcac ggtgcccagt cacgcttcga cgccgaacac
     1501 gatgcgaggt cggaagcggc atgagtgcgg agcaacccgg cacgaacctc gccgcactcc
     1561 ttgatcgagc cgacgaggtg gcaacgaggg gcgacgcgcg agcagcggta tccttctacc
     1621 aggcagcgct gttgcaggcg cgatcagccc gcgggctgga cgccgggatg gtcttacggt
     1681 tgcgacgtgg tcaggagttc atccagctac gcaccaagga attcgagcga acgcttgacc
     1741 aaagtttgcc cactgatcct ccagcggatg cggcggcggc gcttcggctg aaccacgcga
     1801 tcgacatcgt gaaggggacg cgcgaactgt acccgcagca gccttccatg ttttattacc
     1861 cgtatcttcc gcagcggcag ttcttcgagc gacaggaatt tgcgtggacg gttgcactgg
     1921 aggcggccac ccgggaaatc gttcacgagc ttcgcgctct actctccgag ggcgcggact
     1981 ttcgcccgta tgtcgaacgc gacccggagc ggccgccccg cgacttccac ggcctgaccg
     2041 aaaatcgctc gtggacggcg ctttatatct ggaagcatgg ccggttcgtg gacgagattg
     2101 ccgagcgctg cccggcaacc gttcgagcgc ttgaaggagt tccgctctcg cggatcggga
     2161 gtcgcacgcc gtcggtcttt ttttcgcgcc ttgagcctgg cgcgcacatc ccgccgcata
     2221 ccggcatgtt gaacagtcgg ctgatttgcc acctgccgtt gatcgttcct gcgggctgta
     2281 ctctgcgtgt gggaaacgag acgcgtgcct gggaggaagg cgagctactc attttcgacg
     2341 acagcatcga acatgaggca cgcaacccca ccggcgaggt tcgcatcgt
//
LOCUS       DATLEE010000635         2628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7116496, whole genome shotgun sequence.
ACCESSION   DATLEE010000635 DATLEE010000000
VERSION     DATLEE010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7116496"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VM692_13890"
     CDS             complement(<1..362)
                     /locus_tag="VM692_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ53313.1"
                     /translation="MRCHPLSLAVAAFALATTAVSVAQPRTPPREITQVKGDLYRARN
                     GNWYTVFLVTPAGIILGDPINETFSPWLKGELDMRFPGKPVRYVVYSHSHFDHAAGGA
                     VFADTATFVGHENMLRNM"
     gene            complement(454..1200)
                     /locus_tag="VM692_13895"
     CDS             complement(454..1200)
                     /locus_tag="VM692_13895"
                     /inference="COORDINATES: protein motif:HMM:NF038116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor H family protein"
                     /protein_id="HUQ53314.1"
                     /translation="MNARLLPSLAIASLMLGASVSAAQTAAGTEERVTRSAEGGAAEH
                     AADELRDPTTFEVIRRAPADIAVKPRSAAGGGKGLDTLKATFGDSWVYEATTDAFADR
                     DADGYFRYLRVRFDVDSLYNPSWVYAEIYLSADGNAWEHLYSTKDFEVFGSDPDDDYE
                     VETELVTGYAAGLYDVLIEIYDADTNELVDEFGPNESSDFSMLPLEDSTRDGVVVELP
                     EDHGGGGAVSWLGLGGLLGALSLRRRRNAA"
     gene            complement(1337..1699)
                     /locus_tag="VM692_13900"
     CDS             complement(1337..1699)
                     /locus_tag="VM692_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53315.1"
                     /translation="MRLTTIISSGLIMGIAGSALACDLPKLAVIPPKDQVAGKEAAIR
                     ASANAYFTAMQAYTACIQSELSAAGGDSAPDVLKRVLVSRNNTAVAEAEFMMKLFTDN
                     VGPADVPAPAAAPPASGR"
     gene            1832..>2628
                     /locus_tag="VM692_13905"
     CDS             1832..>2628
                     /locus_tag="VM692_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53316.1"
                     /translation="MRCWQLTAACAALALGVLGDSDAQVATQPADAASKWRTSWGHPD
                     LQGTWSSDDVRGIPLQRPEELGTKYELTDAELAERQQGNDSQVARLREGGTAFLAERG
                     VRTFRFTSLVVDPPNGRIPALTAEGQKRNDETQARRRSRPSSWEDRSLYDRCLTRGVV
                     GSILPVIYGNGLEIHQTPNDVVIRYEMIHETRIIPLDAKPRLGDAIKMYMGDARGHFE
                     GETLVVETQNMIGRAAVGNNGNGPPLTPSAQLVERFTRVADDRIDYSV"
BASE COUNT          464 a          853 c          875 g          436 t
ORIGIN      
        1 tccatgttac gcaacatgtt ctcgtggccg acgaacgtcg cagtgtccgc aaacacggcg
       61 ccgccggccg cgtgatcgaa gtggctgtgg ctgtagacca catagcgaac gggcttgccc
      121 gggaagcgca tgtcgagctc gcccttgagc caaggcgaga acgtctcgtt aattgggtcg
      181 ccgaggatga ttccggccgg cgtcacgagg aacaccgtgt accagttgcc gttacgcgcg
      241 cggtagaggt cgcctttgac ttgtgtgatc tcgcgcggcg gcgtgcgcgg ttgcgcaacc
      301 gaaaccgcgg tcgtggctaa agcgaacgcg gcgacagcga gactgagagg gtgacagcgc
      361 acgcgagcac ctgacgacga gtagaacggt gccccggagt atagagcagc ggcggcaagc
      421 agtgcccgcc gccgcttcat tggaagctgc cgactacgcc gcgttgcgac gacgccgtag
      481 cgagagcgcg ccgagcaatc cgccgaggcc gagccacgag acggcgccgc cgccgccgtg
      541 gtcttccggc aactcgacga ccacgccgtc gcgagtcgag tcttcgagcg gcagcatcga
      601 gaagtccgac gactcgttcg gcccgaactc gtcgacgagc tcgttggtgt cggcatcgta
      661 gatctcgatc agcacgtcgt agaggccggc cgcgtagccc gtgacgagct cggtctcgac
      721 ctcgtagtcg tcatcgggat cggagccgaa cacctcgaag tccttcgtcg agtacaagtg
      781 ctcccacgcg ttgccgtcgg cgctcaagta gatctcggca tagacccacg aagggttgta
      841 gaggctgtcg acgtcgaagc gcacgcgcag gtagcggaag tagccgtccg cgtcgcggtc
      901 cgcgaacgcg tccgtcgtcg cttcgtagac ccacgaatcg ccgaaagtcg ccttcaacgt
      961 atcgaggccc ttgccgccgc cggccgcgct gcgcggtttg actgcgatgt cggccggtgc
     1021 ccgccggatg acttcgaagg tcgtcgggtc gcgcagctcg tcggccgcat gctcggcggc
     1081 gccgccttcg gcgctgcgcg tgacgcgctc ttccgtgcct gccgcggttt gggcggcaga
     1141 cacactggcg cccagcatta ggctcgcgat cgcgagtgac ggcagcaagc gtgcgttcat
     1201 gttcagccct ccgggcacag tagtgccgtt agggcctatt tagcgacgcg tatgctgaac
     1261 ggcagctgag gccgccgaag ggcgtcgaac tatggcaaat ggccggaccg ggaccgcccc
     1321 ggccaccgtc ttcctgctag cgcccggacg cgggcggtgc cgctgccggc gcggggacgt
     1381 cggcagggcc gacattgtcc gtgaacagct tcatcatgaa ctcggcctcg gcgacggccg
     1441 tattgttccg cgacacgagc acgcgcttga ggacgtcggg ggccgaatcg ccgcccgcgg
     1501 cgctgagctc gctctgaatg caggccgtat acgcttgcat cgccgtgaag tatgcgttgg
     1561 ccgacgcacg aatcgccgct tccttgccgg cgacctggtc cttcggcggg atcaccgcga
     1621 gcttcggcaa gtcgcacgcc aaggcgctgc cggcaatacc catgatgagg ccgctcgaga
     1681 tgatcgtcgt caatcgcatg gtctagtccc ccgaagatgc ccgtacggct cgttgtgatt
     1741 tctctagtct acacaaatgc ctttgcgttg cggcagcgtc gactgcagtc ccacaatcga
     1801 cgacagccaa gcgtgggcac cggggggagt catgagatgt tggcagctga cggccgcgtg
     1861 cgcggcgctc gcactcggcg ttcttggcga cagcgacgct caagtcgcga cgcagccggc
     1921 cgacgcagcg tcgaagtggc gcacatcatg gggccatccc gatttgcaag gcacgtggtc
     1981 cagcgacgac gttcgcggca ttccgctaca gcggcccgag gagctcggca cgaagtacga
     2041 gctgaccgac gcggagctcg ccgagcgaca gcaaggtaac gactcacaag tcgctcgctt
     2101 acgcgaaggc ggcacggctt ttctggcaga gcgcggcgtg cgcacgttcc gcttcacgtc
     2161 gctcgtcgtc gatccgccga acggacgcat tcctgcgttg acggccgaag gtcagaagcg
     2221 caacgacgag actcaggcgc ggcgcagatc gcggccgagc tcgtgggaag atcgcagcct
     2281 ttacgatcgc tgcctcacgc gcggcgtcgt cggctcgatt ctgccggtca tctacggcaa
     2341 cggcctcgag attcaccaaa ccccgaacga cgtcgtgatt cgctacgaaa tgattcacga
     2401 gacgcggatc attccgctcg acgcgaagcc gcgcctcggc gacgcgatca agatgtacat
     2461 gggcgatgcg cgcggccact tcgagggcga aacgctcgtc gtcgagacgc agaacatgat
     2521 cggccgcgcg gccgtcggta acaacggcaa cgggccgccg ttgacgccgt cggcgcaact
     2581 cgtcgagcgc ttcacgcgcg tggcggacga tcgcatcgac tactcggt
//
LOCUS       DATLEE010000636         8309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3408609, whole genome shotgun sequence.
ACCESSION   DATLEE010000636 DATLEE010000000
VERSION     DATLEE010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3408609"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..2074)
                     /locus_tag="VM692_13910"
     CDS             complement(74..2074)
                     /locus_tag="VM692_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HUQ53317.1"
                     /translation="MRRIAVRGTAEQRTALLASEWLVTNGLGGYASGTIGGAPSRRYH
                     GVLVAALPPPHGRRVLLTDLHEELYVAHERVGLLTARAAEDDEHVPLTEFRLELGLPV
                     WTYDLGGIVVEKRLLMPHGQNTVYVLYELKEGAATARLDVSIATHHRSHDASVATPID
                     LRRPLERTSFGHELALAELPLLKLMVDAARVEYTERQASWPERRYSIEESRGYMDTAA
                     AWTPGLFSLELVRGRPAAIVASVEPWEVVTALSATAALAAERERRRECIDAAAPAARD
                     GLAAELVLAADQFIVTPVGRAAEAARVRARGDDERSVIAGYHWFTDWGRDTMISLEGL
                     TLLTGRAAEAKGILRTFTSSIRDGLVPNLFPEGESQGLYHTADATLWLFHALDRYLAA
                     TSDRALLLALLPQLLDVVERHMAGTRFGIGVDNRDGLLRQGAPGFQLTWMDAKVGDWV
                     VTPRRGKAVELNALWYNALCLMQGWTEQYGGAENVPFADHARRARASFNERFWYEDGG
                     YLYDVIDGERGNEACCRPNQVFAIALPHPILDRERWDPVMKVVRERLLTPVGLRSLAP
                     DDPDYKARYYGDLRARDAAYHQGTVWAWLIGPYIDAELKLPGADRAKLRELIAGFDQH
                     LDEACVGQISEVFDAEAPFLPRGCVAQAWSVAEVLRCWLKLA"
     gene            complement(2128..2415)
                     /locus_tag="VM692_13915"
     CDS             complement(2128..2415)
                     /locus_tag="VM692_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53318.1"
                     /translation="MSKSKRTTDHAEIRKWAEARQGTPARVQGTGESDDAGLLRIDFP
                     ERESEQDDDDELEEITWDEFFEKFDEKRLAFVYQEQTAAGQQSRFNRFVSR"
     gene            complement(2503..3375)
                     /locus_tag="VM692_13920"
     CDS             complement(2503..3375)
                     /locus_tag="VM692_13920"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HUQ53319.1"
                     /translation="MNVSQSFKQPKLAVIAAIVAAAFAGVSAAQQQEPTRQNANRPQT
                     GQSTQVARSDAGLQTVEQFLQSKTLRVSKLVGLDLQTRSGDSLGEVKDIIPSAAAGRG
                     MQLIVSTGGVGADQKLIAVPFNEVQINADGDELYTNRTKEQLASAPAVTLDARTSDTA
                     AAANRSAAPQPGAGADTRTPPPAGAAAGRGATSTQQPVADLVGADVYGSGDDKVGEVD
                     DIVISTAGADSIRAVLQVGGVAGIGEKRISLPLSQLAVERSGNDEPKLRVALEREALD
                     RMPEFKYEEQTSAL"
     gene            3532..3696
                     /locus_tag="VM692_13925"
     CDS             3532..3696
                     /locus_tag="VM692_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018948711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HUQ53320.1"
                     /translation="MIYYALVFLVVALIAGVLGFSGIAGTAANIAWILFVVGLVLAVV
                     FFVRGRGSSI"
     gene            3700..3876
                     /locus_tag="VM692_13930"
     CDS             3700..3876
                     /locus_tag="VM692_13930"
                     /inference="COORDINATES: protein motif:HMM:NF014160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallothionein"
                     /protein_id="HUQ53321.1"
                     /translation="MAGQGRCSHHGCSCTVEEPHAVLQRGKAYCSDGCAAGRGCEHGD
                     CDCRPHDAAPARSD"
     gene            3986..6124
                     /locus_tag="VM692_13935"
     CDS             3986..6124
                     /locus_tag="VM692_13935"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HUQ53322.1"
                     /translation="MATPRKTKNVTTETAERPVTARYGDQSVELGSGGETHQRAVDDH
                     ELTTQLGTPIADDQNSLRIGPRGPALLEDFHFREKLFHFDHERIPERAAYARGFGAHG
                     FFETYESLADVTTADLFQRAGEMTPAFVRFSTLIGSSGSFDLARDVRGFAVKLYTQRG
                     NWDLVGNSMPVFFIQDPIKFPDLVHAVKPAPDRGFPQAQSAHDNFWDFISLMPESMHM
                     IMWLMSDRALPRSFRFMEGFGVHTFRLVNEAGRSTFVKFHWTPKLGLQSVVWNEAVKI
                     NGADPDFHRRDLWDAIEAGDFPEWELGMQLFDAETEALLPFDPLDPTKLITEELVPVR
                     RVGRLVLDRNVKNFFAETEQVAFGTQNIVPGIEFSNDPLLQGRNFSYLDTQLKRLGGA
                     NFAQLPINAPQCPFRHFQQDGHMAMFNPSGRVNYAPNSWQAGRMAGESTNGGFESYRD
                     EVAGTKSRARPESFADHYNQARQFYLSQTPLERQHIAAALVFELSKVETRAIRSRVVA
                     HLLNVDDKLAMNVAEGLRLDALPPPATRIVPPRADLPESPGLSIMRKRPQTFTGRRLG
                     VLVTDGVDGSFLQQLEAEFNEVGATVELIAPAIGGVKTEDGSWVEAHKTLATAPSVLF
                     DAVALVVSPQASKEIASSLLACAFVSDAFAHCKFIAYTGDARPLLERALAGTPLDAGC
                     KQVETTMAAAAFAARCAELRFWAREPLLGL"
     gene            6121..7077
                     /locus_tag="VM692_13940"
     CDS             6121..7077
                     /locus_tag="VM692_13940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUQ53323.1"
                     /translation="MSGGTRHSLLAGAGCGLLLIGSSSPPSSLPLDRAGQGEARVSGA
                     DATVTPGEQWFKAMRTALDEPLRVDIPFAERTQADGRATAFSFKAAAGQTLEITVASG
                     ADAADDGIYVEVFRVVDVLGEGLHERLAALRPTATSLRTRLPSDGTYHVLVQGGSASA
                     SPRRFTVELGAALPFPVVGAHEDAIRSLFGASRDSGERNHLGVDIFVQRLTPVLAVAA
                     GRAMPRKDALGGNTVWLNTPGTSYYYAHLDRVAVREEQQVKVGDVLGYVGNTGNARHV
                     SSHLHFGVYRWGKEPIDPLPLIVGQRFDKRTAAAALAAGAGG"
     gene            complement(7074..7229)
                     /locus_tag="VM692_13945"
     CDS             complement(7074..7229)
                     /locus_tag="VM692_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53324.1"
                     /translation="MRIYDIEFDIDSFLDEMEEMQRARRQRQQQIHRSSEMGGSVGEG
                     PRDRDSD"
     gene            complement(7574..7732)
                     /locus_tag="VM692_13950"
     CDS             complement(7574..7732)
                     /locus_tag="VM692_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53325.1"
                     /translation="MAEDKRNRGDQQQGGNQSQDRQRGNKDRKPGGSMESPDSSRTPQ
                     QDDEEQAE"
     gene            complement(7803..>8309)
                     /locus_tag="VM692_13955"
     CDS             complement(7803..>8309)
                     /locus_tag="VM692_13955"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUQ53326.1"
                     /translation="IEPGSPSEQAGIAAGDVIVAIDEDAIDSSADLRNEVGLVRAGEQ
                     VAVTFVRDGERRTVRATVAAETESDPPAARAADPASVSLLAGAALMELPSDHPAYGRV
                     RGVWVSAVAPGSAAARFGLHPDDLITGVNRQSVTSLADLTARLRDAKTPVALQVQREG
                     RSLFLLVR"
BASE COUNT         1298 a         2740 c         2800 g         1471 t
ORIGIN      
        1 cgcggtcgtg gcgcgcgttc cacgcggtgg tctccaccct gcgcgccccg tttgcccaac
       61 gccgcgactg acgttatgcg agtttgagcc agcagcggag cacttccgcg acgctccacg
      121 cctgcgcgac gcagcctcgc ggcaggaacg gcgcttcggc atcgaacacc tcgctgatct
      181 ggccgacgca ggcttcgtcg agatgctgat cgaagccggc aatcaactcg cgtaatttcg
      241 cgcgatccgc ccccggcagc ttcaactccg catcgatgta gggaccgatc agccacgccc
      301 acactgtgcc ctggtggtag gccgcgtcac gcgcgcgcag gtcgccgtag taacgcgcct
      361 tgtagtcggg atcgtctggc gccagcgagc gaagaccgac cggcgtgagc agccgttcgc
      421 ggacgacttt catcaccggg tcccatcgct cgcgatcgag gatcggatgc ggcagcgcga
      481 tcgcgaacac ctggttcggc cggcagcagg cctcgttgcc gcgttcaccg tcgatcacgt
      541 cgtagaggta cccgccgtct tcgtaccaga agcgctcatt gaatgacgcg cgggcgcgcc
      601 gcgcatgatc ggcgaacggc acgttttccg caccgccgta ctgctcggtc cacccctgca
      661 tcaggcacag ggcgttgtac cagagcgcgt tcagctccac cgccttgccg cgccgcggcg
      721 tcacgaccca gtcgccgacc ttggcgtcca tccaagtcag ctggaatccc ggcgcacctt
      781 gccgcagcaa cccgtcgcgg ttgtcgacgc cgatgccgaa gcgcgtgccg gccatgtgac
      841 gttccacgac gtcgaggagt tgcggcagca gcgccagcaa cagcgcgcga tcgctcgttg
      901 ccgcgaggta gcgatcaagc gcgtggaaca gccacaacgt cgcgtcggcc gtgtggtaga
      961 ggccttggga ttcgccttcg gggaacaggt tcggcacgag accgtcgcgg atcgagctcg
     1021 tgaacgtgcg caaaatgccc ttggcctcgg ccgcgcgtcc cgtcagcagc gtcagccctt
     1081 cgaggctgat catcgtgtcg cggccccaat cagtgaacca gtgatagccg gcgatcacgg
     1141 atcgctcgtc gtcgccgcgc gcgcgtacgc gcgccgcttc cgcggcgcgg ccgactggcg
     1201 tcacgatgaa ctgatcggcc gccagcacga gctcggcggc aagcccgtcg cgcgcggccg
     1261 gagccgccgc gtcaatgcat tcgcgacggc gctcgcgctc cgccgcgagt gccgccgttg
     1321 cgctgagcgc cgtcacgact tcccacggct caacggacgc gacgatcgcc gcgggtcggc
     1381 cgcgaacgag ctcgagcgag aagaggccgg gcgtccacgc cgccgctgta tccatatagc
     1441 cgcgcgattc ttcgatcgag tagcgacgct cgggccagct cgcttgccgc tccgtgtact
     1501 cgacacgcgc cgcatcgacc atgagcttca acaacggcag ctccgcgagc gcgagctcgt
     1561 ggccgaagct cgtgcgctcg agcggccggc gtaagtcgat cggcgtcgcc accgacgcgt
     1621 cgtgcgaacg gtggtgcgtg gcgatcgaga cgtccaggcg cgccgtagcg gcgccctctt
     1681 tgagctcgta gagcacgtag accgtgttct ggccatgcgg catcaacagc cgcttctcga
     1741 cgacgatgcc gccgaggtcg tacgtccaga ccggcaagcc gagctcgagc cgaaattcgg
     1801 tgaggggcac gtgctcgtcg tcttctgcgg cgcgcgctgt caggaggccg acgcgctcgt
     1861 gggcgacgta tagctcctcg tgaaggtcgg tcaggagcac acggcggccg tgcggcggcg
     1921 gcagcgccgc gaccagcacg ccgtgataac ggcgcgacgg tgcaccgccg atcgtccccg
     1981 acgcatagcc gcccaggcca ttggtaacga gccactcgct cgccaacagc gcggtgcgtt
     2041 gctcggccgt gccgcgcacc gcgattctgc gcacgggccc ggcggccgcc gggagcgcag
     2101 ccaggcgatg ctggctcgcc gcgcgcatca gcggctgacg aatctgttga agcggctctg
     2161 ctgcccggcc gccgtctgtt cttgatagac gaaggcgagg cgcttctcgt cgaacttctc
     2221 gaagaactcg tcccaggtga tttcctcgag ctcgtcgtcg tcgtcttgtt cgctttccct
     2281 ttccgggaag tcgatgcgca acaagccagc gtcgtccgat tcgcccgtgc cttgcacacg
     2341 tgccggcgta ccctggcggg cctccgccca tttgcgaatc tcggcgtgat ccgtcgttcg
     2401 cttcgatttg ctcactggtg ctccccgcgg ctcgacgcga agaccggccg cggttgcccg
     2461 cgaccggtct cgagtcagcc tgtcattgac gttggcgccg tatcagagcg ccgacgtctg
     2521 ctcttcgtac ttgaactcag gcatgcggtc gagcgcctcg cgctccagcg cgacgcgcag
     2581 cttcggctcg tcgttgccgg aacgctcgac ggccaactgg ctcaacggca gcgagatgcg
     2641 cttctcgcca atgcctgcga cgccgccgac ctgcagcacc gcccggatgc tgtcggcgcc
     2701 ggcggtcgag ataacgatat cgtcgacctc gccaaccttg tcgtcgccgc tgccgtaaac
     2761 gtctgcgccg acgagatcgg cgacgggttg ttgcgtcgaa gtcgcgcctc gccctgcggc
     2821 cgcaccggcc gggggcggcg tgcgcgtgtc ggcgccggcg ccgggttgcg gcgctgcgct
     2881 gcgattggct gctgcggcag tgtcgctcgt gcgcgcgtcg agcgtcacgg caggcgccga
     2941 cgcgagctgt tccttcgtgc ggttcgtgta gagctcgtcg ccgtcggcgt tgatctggac
     3001 ctcgttgaac gggaccgcga tcagtttctg gtcggctccg acgccgcccg tcgagacgat
     3061 caactgcatg ccgcggcccg cagccgcgct cggaatgatg tccttgacct cgcccaagct
     3121 gtcgccgctg cgcgtttgca ggtccaagcc cacgagcttg ctgacgcgca gcgtcttgct
     3181 ctgaagaaac tgctcgacgg tctgcagacc cgcgtcgctg cgcgcgacct gcgtcgattg
     3241 ccccgtctgc gggcggttcg cattctgacg cgtcggctct tgctgctgag ccgcgctcac
     3301 gcccgcgaaa gccgccgcga caatggccgc gatgacggcg agcttcggtt gtttaaacga
     3361 ttgggaaacg ttcatgtcat tcacctcgct tatgtcactg ctgtgcaggg gttgcggccc
     3421 gttaggccaa gatcgcggcc gataggtgca agacatatgc cagcgggagc gagcagtggc
     3481 acagagcttg caacttgtgg gtcgcaggtt gggcaatagg agagaggatc catgatttat
     3541 tacgcgctcg tgttcttggt ggtagcgctg attgccggcg tgctggggtt ttccggcatc
     3601 gcgggtacgg ccgccaacat cgcttggatc ttgttcgtag tcggcttggt actcgcggtc
     3661 gtgttcttcg tacgtggccg cggctcgagt atctagctaa tggccggtca ggggcggtgc
     3721 tcacaccatg ggtgctcctg cacggtcgaa gagccgcacg ccgtgctgca gcgtggcaaa
     3781 gcgtactgca gcgacggttg tgcggccggg cgagggtgcg agcacggcga ctgcgactgc
     3841 aggccgcacg atgccgcgcc ggcgcgcagc gattgatggt gccggaagca ggcgtgcggg
     3901 ccgcggcgct acgagcggcg cacgcctccg cgttcccgcg cgcgcgggat gacgtgcgcg
     3961 cgggaacgcg aggggccaac ctggaatggc gacaccgagg aagacgaaaa atgtaacgac
     4021 ggagacggcc gagcgccccg taacagcgcg ctacggcgac caatccgtcg agctcggctc
     4081 gggcggtgaa acgcatcaac gcgccgtcga cgaccacgag ctcacgacgc aactcggcac
     4141 gccgatcgcc gatgatcaaa acagcttgcg catcggcccg cgcgggcctg cattgctcga
     4201 agattttcac tttcgcgaaa agcttttcca tttcgaccac gagcgaatac cggagcgcgc
     4261 tgcgtatgcg cgtggctttg gcgcgcacgg tttcttcgaa acctacgagt cgctggccga
     4321 cgttacgacg gccgatctct ttcagcgcgc gggggaaatg acgccggcat tcgtgcgctt
     4381 ctctaccctg atcggcagca gcggctcgtt cgatctcgcg cgcgacgtgc gcggttttgc
     4441 cgtgaagctc tatacgcaac gcggcaactg ggatctcgtc ggcaacagca tgccggtgtt
     4501 cttcatccag gacccgatca agtttccgga tctcgtgcac gccgtgaagc cggcgcccga
     4561 ccgaggattt ccgcaggcgc aatcggctca cgacaacttt tgggacttca tctcgctgat
     4621 gcccgaaagc atgcacatga tcatgtggct gatgtcggac cgcgccctcc cgcgctcgtt
     4681 ccgcttcatg gagggcttcg gcgttcacac gttccggctc gtcaacgaag ccggccgctc
     4741 gacgttcgtc aaattccact ggacgccgaa gctgggactg cagtcggttg tctggaacga
     4801 agcagtcaag atcaacggcg cagatcccga ctttcaccgc cgcgacctat gggatgcgat
     4861 cgaggcgggc gactttcccg agtgggagct cggcatgcag ctgttcgacg ccgagaccga
     4921 agcgctgctg cccttcgatc cgctggatcc gacgaagctg atcacggagg agctcgtgcc
     4981 cgtgcgccgc gtcggcaggc tcgtgctcga ccgcaacgtc aagaacttct tcgccgagac
     5041 ggagcaggtg gcgttcggga cgcagaacat cgtgcccggt atcgagttca gcaacgaccc
     5101 gttgctgcag gggcgcaatt tctcgtatct cgacacgcag ctgaagcggc tcggcggggc
     5161 gaacttcgcg cagctgccga tcaatgcgcc gcagtgcccg ttccgccatt tccagcaaga
     5221 cgggcacatg gcgatgttca atccgtccgg ccgcgtgaat tacgcgccga actcgtggca
     5281 ggcggggcgc atggcaggcg agagcacgaa cggcggcttc gagtcgtatc gtgatgaagt
     5341 ggccggcacg aagagcaggg ctcgacccga gagcttcgcc gatcactaca accaagcgcg
     5401 gcagttctac ttgagccaga cgccgctcga gcgacagcac atcgcggcgg ccctcgtttt
     5461 cgagctcagc aaggtcgaga cgcgagcgat tcggtcgcgt gtcgtcgcgc atctgttgaa
     5521 cgtcgacgac aagctcgcga tgaacgtagc cgaaggcttg cggctcgacg cattgccgcc
     5581 gccggcgacg cggatcgtgc cgccgcgggc cgacctgccg gagtcgccgg ggctcagcat
     5641 catgcgcaag cgcccgcaga cgttcacggg ccgccggctc ggggtgctcg tgaccgacgg
     5701 cgtcgacggc agcttcttgc agcagctcga ggcggagttc aacgaggtcg gcgcgacggt
     5761 cgagctcatc gcaccggcga tcggcggcgt caagaccgag gacggctcct gggtcgaagc
     5821 gcacaagacg ctggccaccg cgccctccgt gttgttcgac gcagtagcgc tcgtcgtctc
     5881 gccgcaggcc agcaaggaga ttgcgagcag cttgctcgcc tgcgccttcg tctcggacgc
     5941 gttcgcgcat tgcaaattca tcgcctatac gggcgacgcg cggccgttgc tcgagcgtgc
     6001 gctcgcggga acgccgctcg acgccggctg caagcaggtc gagacgacga tggcggccgc
     6061 ggcgtttgcg gcccgctgcg ccgagctgcg attctgggcc cgcgagccgt tgctcggcct
     6121 atgagcggcg ggacccgcca ttccctactc gcaggcgcag gttgcgggct gctgctgatc
     6181 ggcagctcga gcccgccgtc gtcgttgccg ctcgatcgcg cggggcaggg cgaggctcgc
     6241 gtcagcgggg ccgatgcgac cgtgaccccc ggcgagcagt ggttcaaagc catgcgtacg
     6301 gcgctcgatg agccgctccg cgtcgacatt ccgttcgcgg agcgaacgca ggccgatggg
     6361 cgtgccacgg cgttctcgtt caaagcggca gcggggcaga cgctcgagat aacggtggcg
     6421 tccggcgccg acgcggccga cgacggaatc tatgtcgagg tgtttcgcgt cgtcgacgtc
     6481 ttgggcgagg gactgcacga gcggctggcg gcgttgcggc cgacggccac gagtctgcga
     6541 acgcggttgc cgagcgacgg cacttatcac gtgctcgtgc aagggggcag cgcgagcgca
     6601 agtccgcgcc gcttcaccgt ggagctcggg gcggcgctgc cgttccccgt cgtgggagcg
     6661 cacgaggacg cgatccgcag cctatttggt gcgtcgcgcg actcggggga gcgcaatcac
     6721 cttggggtcg atatcttcgt gcagcggctg acgccggtgc tggccgtggc ggcgggacgc
     6781 gcgatgccgc gcaaggacgc gctgggcggg aatacggtgt ggctgaatac gccgggaacg
     6841 tcgtactact acgctcattt ggatcgcgtc gccgtgcgcg aggagcagca ggtcaaggtc
     6901 ggggacgtgt tgggctacgt gggcaatacc ggtaatgcgc ggcatgtctc gtcgcatctg
     6961 cacttcggtg tctatcgctg gggtaaggag cccatcgatc cgttgccgct gatcgtgggc
     7021 cagcgcttcg acaagcgaac agcggcggcc gcgttggcgg ccggcgcggg gggctaatcg
     7081 ctgtcgcggt ctctagggcc ttcgcctacc gagccgccca tttcgctgga ccgatgaatc
     7141 tgctgctggc gctgacggcg cgctcgctgc atctcttcca tctcgtcgag aaagctgtcg
     7201 atgtcgaatt cgatgtcgta gatccgcatg gccgcgctcg tcctctatgc agtgttatag
     7261 ggcgggtcat ctccccggtc cgagatataa aagcaaactc cgtgccagcc gcaccgctcg
     7321 cgcgcagtgg cgatgacgcg acgcacgccg ctgcaagcgc atcgacgctg cgtgcttttc
     7381 gatacaaaga gtgctgggcg cgaaatagcg ggggttgcaa actttagtgc gtgccccgaa
     7441 cggcagggtt gcaaactttg caagctcttt ccggcttgcg tcgcgcccgt ttggggtcgt
     7501 gttcttgttg acagcggatc gtgtggctcg aggcacgtac gggcagcggc tgagccgccg
     7561 cccgtacgtt tggctactct gcctgttcct cgtcgtcctg ctgcggagtg cggctgctgt
     7621 ccggggactc catgctgccg cccggtttgc ggtctttgtt accgcgctgc cggtcctggc
     7681 tctggttgcc gccctgttgt tgatccccgc gattacgctt gtcttcggcc atgagattcc
     7741 ctcttgctgg ttgcggtcga atgaaccgca caggcaaaag tgcaactccc gtgccagtgg
     7801 ttttagcgga cgagcaagaa cagcgagcgc ccttcgcgct gcacctgcag cgccaccggg
     7861 gtcttcgcgt cgcgcagcct cgcggttaga tcggccagcg aggtgacaga ctggcggttg
     7921 acgccggtga tcaaatcgtc ggggtgtaag ccgaaacgcg ccgccgccga gcccggcgcg
     7981 accgcgctga cccagacgcc gcgcacgcgg ccataggccg ggtgatcgct cggcagctcc
     8041 atgagggccg cgcccgccaa caacgaaacc gacgctgggt ctgcagcgcg agcagcgggc
     8101 gggtcgcttt cggtttccgc ggcgaccgtc gcgcgcacgg ttcgccgttc accatcgcgg
     8161 acgaacgtca cggcgacttg ctcgccggca cgcacgagac cgacttcgtt gcgcaggtcg
     8221 gccgagctat cgattgcgtc ctcgtcgatc gcaacgatga cgtcgccggc tgcgatgccg
     8281 gcttgctcgg aaggggagcc cggctcgat
//
LOCUS       DATLEE010000637         4396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10082171, whole genome shotgun sequence.
ACCESSION   DATLEE010000637 DATLEE010000000
VERSION     DATLEE010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10082171"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..763
                     /gene="folE"
                     /locus_tag="VM692_13960"
     CDS             164..763
                     /gene="folE"
                     /locus_tag="VM692_13960"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HUQ53327.1"
                     /translation="MPAKRIAKPTRAKAEAAVRTLLEWIGDDPDREGLLGTPGRVVRA
                     YEDWFSGYNDDPLEFLRRTFREVDGYDEMIVLRNISFESHCEHHMAAIIGSVHIGYLP
                     DRKVVGISKLARVVETFARRLQVQESLTAQIAHCIQDVLKPRGVGVVVEGVHQCMTTR
                     GVRKHGVSMITSQLLGHFRSDPRTRAEFLSMLGRAGERA"
     gene            953..1477
                     /locus_tag="VM692_13965"
     CDS             953..1477
                     /locus_tag="VM692_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HUQ53328.1"
                     /translation="MKNLLTLERRAAGFTLIELMIVVAIIGILAAIAIPAYQTYTIRA
                     QVAEGLNMATHSKAPVADSFFNRGEAPATRAEAGLSANATDTVGKYVQSVNIVNGVLV
                     ATFGNEANALITGLTVTTTPYETADRSIVWRCGTAPIPTGIVGPLGASGSGNQAVYIA
                     PTVPEQYLPSSCRP"
     gene            complement(1482..2081)
                     /locus_tag="VM692_13970"
     CDS             complement(1482..2081)
                     /locus_tag="VM692_13970"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HUQ53329.1"
                     /translation="MRESVAKRRIIVAMTGATGAVYGKRLLEELRRVGGVEIHVVISK
                     AGVVSAAAELDVGRRELEALADVAHAEKDIGASIASGSFKTDGMIIAPCSMRTLAAIA
                     TGVTDNLVTRAADVVLKERRRLVLLARETPLTLVHLRNMVTITEMGGVVFPPVPAFYA
                     KLDSIDAMIDQTIGRVLDLFDIDSPLVRRWSGVGAPADD"
     gene            2182..2859
                     /gene="modB"
                     /locus_tag="VM692_13975"
     CDS             2182..2859
                     /gene="modB"
                     /locus_tag="VM692_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HUQ53330.1"
                     /translation="MPEVELGPVWLSLQLATVTVIVLLIVCTPLAWWLAFTRSRARTV
                     IEAVVALPLVLPPTVLGFYLLVLMGPAGLVGGAWLRLTDTTLTFSFAGLVVASVLYSF
                     PFAVQPLQGAFQSVGRAPLEAAATLGAGPFDRFLHVASPLALRGYVTAIVLSFAHTLG
                     EFGVVLMVGGNIPSKTRVISIAIYEHVETLDYAQAHVLAAGLLVFSFLVLLFVYSLTR
                     RAPVVRV"
     gene            2864..3937
                     /gene="modC"
                     /locus_tag="VM692_13980"
     CDS             2864..3937
                     /gene="modC"
                     /locus_tag="VM692_13980"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019407256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HUQ53331.1"
                     /translation="MSTLALNVALERTSFTLQIDHELALEGITALFGPSGAGKTTLLR
                     IIAGLEHDVRGRVTFDGEAWQTDAASVPPHRRGIGYVFQDGRLFPHLTVEQNLRFAVR
                     RNSRRSIELSAAVEALDLGDLLERRTPSLSGGEQQRVAIARALLTSPRLLLMDEPLSS
                     LDVARKKDIVPHIEKLPALFGIPVLYVTHNVDEVARLAQDVVLLVAGKVAAHGSVAEV
                     FERTDLGAFTGGLEAGVVLRTEVTAYEGGVATLRVGLQQLRVPMAAAHVGATRPIRIH
                     ARDVAIATVRPEKLSIRNVLSARILRIETGANLNVELLLDVDGEHLRARITRDAHDEL
                     ELATGREVFALVKSVALESSLNA"
     gene            3948..>4396
                     /locus_tag="VM692_13985"
     CDS             3948..>4396
                     /locus_tag="VM692_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ53332.1"
                     /translation="MNVTNLDSVVFGINDLAACRRFWTDFGLAESRAPDGATVFSCKN
                     GSTVVLRNADDPKLPQPIEPGATQREAVFGVKSAADLTAIANELERDRPVRIDADGSV
                     HAADPLGFGIGFRVAQRKPITAPELKFNTPGRPDRINTRGQFFDSAAP"
BASE COUNT          755 a         1470 c         1419 g          752 t
ORIGIN      
        1 agatgacgag ctcccatgca tgctctcgtg cgcgctctgt tgatcgatcg cgcgtcaata
       61 tgccagcggc caacgtcggt tgcaccaccc ggcgagccag aattagactg cggacctccc
      121 accgcaaacg agacggagcg ccgtggcccg acgcaataag actttgcccg ccaagcgcat
      181 agccaaacct acgcgcgcca aagcagaagc ggccgttcgc acgctgctcg aatggatagg
      241 ggacgacccg gaccgcgagg ggctcctcgg cactccgggc cgcgtcgtgc gcgcgtacga
      301 ggactggttc agcggctaca acgacgaccc gctcgagttc ttgcggcgca ccttccgtga
      361 agtcgacggt tacgacgaga tgatcgtgct gcgcaacatc agcttcgaat cgcactgcga
      421 gcaccacatg gccgcgatca tcggctccgt gcacatcggg tatctgccgg accggaaggt
      481 cgtcggcatc agcaagctcg cgcgcgtcgt cgagacgttc gcgcgccggc ttcaagtgca
      541 ggagtcgctg acggcgcaaa tcgctcattg catccaagac gtgctgaaac ctcgcggggt
      601 gggcgtcgtc gtcgaaggcg ttcatcagtg catgaccacg cgcggcgtgc gcaagcacgg
      661 tgtgtcgatg atcacgagcc agttgctggg gcacttccgc tccgatccgc gcacgcgcgc
      721 cgaattcctg tcgatgctgg gcagagccgg cgagcgggct tgaacgtcgg ctcgcgacat
      781 aaccgcggcg gcttggtcgc gaagtgacgc gcatcgtcag cttgggtgaa cataaaacag
      841 gttggcgccc cgccgagccg cactgcaccg tgcctggcat acgtcttgct gatcctcttg
      901 cacagtgcac gcgtgtaacg caacacgctg cgggaacacg accgacattg cgatgaaaaa
      961 cctactcaca ctcgagcgcc gcgccgccgg cttcacgctg atcgagctga tgatcgtcgt
     1021 ggcgatcatc ggaattcttg ccgccattgc gattccggcc taccagactt atacgatccg
     1081 cgcgcaggtt gccgaaggcc tcaacatggc gacgcattcg aaggctccgg tcgcagattc
     1141 gttcttcaac cgcggcgaag cgccggccac tcgtgccgaa gctggcctgt cggctaacgc
     1201 gacggacacg gtcggcaagt acgttcagtc cgtgaacatc gtcaacggcg tgctcgtcgc
     1261 cacgttcggc aacgaggcaa acgcgctgat cacgggccta acggtcacga cgacgccgta
     1321 cgagacggcg gaccggagca tcgtttggcg ctgcggcacg gcaccaatac cgacgggcat
     1381 cgtcgggccg ctcggcgcct cggggagcgg caaccaagcc gtctacatcg caccgaccgt
     1441 gccggaacag tacctgccct cgtcgtgtcg accatagcgg gtcagtcgtc ggcgggcgcg
     1501 ccgactcccg accagcgccg cacgagcggc gagtcgatgt cgaacaaatc gagcactcgg
     1561 ccgatcgtct gatcgatcat cgcgtcgatg ctgtcgagct tggcgtagaa cgccggcacg
     1621 ggcggaaaca cgacgccgcc catctccgtg atggtgacca tgttgcgcag gtgcacgagc
     1681 gtcagcggcg tctcgcgcgc caacaagacg aggcgccgac gctccttcag cacgacgtcg
     1741 gccgcgcgcg tgacgagatt gtccgtcacg ccggtcgcaa tcgccgccaa cgtccgcatg
     1801 gagcacggcg cgatgatcat gccgtcggtc ttgaaggagc cgctcgcgat gctggcaccg
     1861 atgtctttct cggcatgagc gacgtcggcc agcgcttcga gctcgcgccg cccaacgtcg
     1921 agctcggcgg ccgcgctcac gacgccggct ttcgaaatca cgacgtgaat ctcgacgccg
     1981 ccgacgcggc gtagctcttc caatagtcgc ttgccgtaga cggcgcccgt cgcgcccgtc
     2041 atcgcgacga tgatgcgccg cttcgccacg ctctcccgca tcggccgaga ataccacgca
     2101 gctttatcgc gaccggcgcg tacgttgaca atgcggcgcg gtgcgtggct acatggaccg
     2161 ccacgatcgc ggacgattga gatgccggaa gtcgagctcg gacccgtatg gctcagtctg
     2221 cagctcgcaa ccgtcaccgt catcgtgctg ctgatcgtct gcacgccgct cgcgtggtgg
     2281 ctcgcgttca cacgaagtag agcgcgcacg gtcatcgagg ccgtggtcgc tttgccgctc
     2341 gtcctgccgc cgaccgtcct tggcttttat ctgctcgtgc tgatgggtcc ggccggtctc
     2401 gtcggcggcg catggctgcg cctcaccgac acgacactga cgttctcgtt cgcgggcctc
     2461 gtcgtcgctt cggtgcttta ttcgtttccg ttcgcggtgc agccgctgca gggcgcgttt
     2521 caaagcgtag gccgggcgcc gctcgaggcc gccgcgacgc tcggcgcggg ccccttcgat
     2581 cgcttcttgc atgtcgcctc gccgctggca ttgcgcggct acgtcacggc tatcgtgttg
     2641 tcgtttgctc acacgctcgg cgagttcggc gtcgtgctca tggtcggcgg caacatcccc
     2701 agtaaaacga gagtgatctc gatcgcgatc tacgagcacg ttgagacact cgactacgcg
     2761 caggcgcacg tactcgctgc cggcctgctc gtgttctcgt tcctcgtctt gctgttcgtc
     2821 tattctctga cgcgccgcgc gccggtcgtg cgggtctagc atcatgagca cgctagcgct
     2881 gaacgtcgca ctcgagcgca cgagcttcac gctgcagatc gatcacgagc tcgcgctcga
     2941 gggaatcacg gcgctgttcg gaccgagcgg cgcaggcaag acgacgctgc tgcgcatcat
     3001 cgccggtctc gagcacgacg tgcgcggccg cgtgaccttc gacggcgaag cttggcaaac
     3061 ggacgcggcc agcgttccgc cgcatcggcg gggaatcggc tatgtgttcc aagatggccg
     3121 tctgttcccg cacctgacgg tcgagcaaaa tctgcgcttc gccgtaaggc gcaactcgcg
     3181 ccggtccatc gagctgtcag cagcggtcga agcgctcgac ctcggggacc tactcgagcg
     3241 ccgcacgccg tcgctgtccg gcggcgagca gcaacgcgtg gcaatcgcgc gggctctgct
     3301 gacgagcccg cgactgttgc tgatggacga gccgctgtcc tcgctcgacg tcgcgcgcaa
     3361 gaaagacatc gtgccgcaca tcgagaagct gccggcgttg ttcggcatcc cggtcctgta
     3421 cgtcactcac aacgtcgacg aggtcgcgcg gctggcgcag gacgtcgtgc tgctcgtagc
     3481 cggcaaagtc gcggcgcacg gcagcgtggc tgaggtgttc gagagaacgg acctcggagc
     3541 gttcacgggc ggcctcgaag ccggagtcgt gctgcgcacg gaagtgacgg cctacgaggg
     3601 cggcgtcgcg acgctgcgcg tcggcctgca gcagctgcgt gtgccgatgg ccgccgcaca
     3661 cgtcggcgcc acacgcccga taagaatcca cgcgcgcgac gtcgcaatcg cgacggtgcg
     3721 ccccgagaag ctcagcattc gtaacgtgtt gagtgcgcgg atcctgagga tcgagacagg
     3781 cgccaacctg aacgtcgagc tattgctcga cgtcgacggc gagcacttac gtgcgagaat
     3841 tacgcgcgac gcacacgacg agctcgagct cgcgaccggc cgcgaggtct ttgcgttggt
     3901 caagagcgtg gcgctcgaga gctcgctgaa cgcataagga cgtgaacatg aacgtgacga
     3961 atctcgattc cgtggtcttc gggatcaacg atctcgcggc ttgccgccgc ttctggaccg
     4021 acttcggcct cgccgagagc agggcgcccg acggcgccac ggttttcagc tgcaagaacg
     4081 gctccacggt cgtgctgcgc aacgcagacg atccaaagtt gccgcagccg atcgagcccg
     4141 gcgcgacgca gcgcgaggcg gtcttcggcg tcaagtcggc cgcggatctg acggccatcg
     4201 cgaacgagct cgagcgcgat cgccccgtgc gcatcgacgc cgacggcagc gtgcacgcgg
     4261 cggacccgct cgggttcggc atcgggttcc gcgtcgcgca gcgcaagccg atcacggcac
     4321 cggagctcaa gttcaatacg ccggggcgtc ccgatcgcat caatacgcgc gggcagttct
     4381 tcgactcggc ggcgcc
//
LOCUS       DATLEE010000638         3099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7842299, whole genome shotgun sequence.
ACCESSION   DATLEE010000638 DATLEE010000000
VERSION     DATLEE010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7842299"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VM692_13990"
     CDS             <1..528
                     /locus_tag="VM692_13990"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin"
                     /protein_id="HUQ53333.1"
                     /translation="SSVALSGGMLAVGAPGEASSAKGIAGNQNDNSAASSGAAYVFER
                     AAGAWQQRASIKASNAEAGDGFGSAVGLSRNWLAVTAPEEDASATGVGGAQGDNGKAD
                     SGATYLFARDGSGTWTQELFIKSANSDANDRFGRTVAFSPGGLIVGSPFEDSHTMANP
                     ASNTASASGAAYVFE"
     gene            complement(546..1925)
                     /locus_tag="VM692_13995"
     CDS             complement(546..1925)
                     /locus_tag="VM692_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53334.1"
                     /translation="MNARTSTFALAAILSAAPVMAQDMPDIGFKSVGRGRPLAASVHD
                     QKEVGPGWIGNPFQPNANLKLDGFRPNELPANVKPLPVDIFNTTDFYADKELWSDPRY
                     FRCNSPMATEIQRGILSPNPLTNDTKQGPWGHCELGLPREAIVSPYGFKTAQEHYEAL
                     LKETRGRGGPQKYTFQNFPAVEWNGVYERPVAGPRNQQTWYWGAHTQISTLLSLLKPE
                     YQQRTVQEAYHQVRGNAYWPSTFCQPEGIMRRFYPYSVWEHYIIATPDLVQITAGVAR
                     NFITNIHVGREFNMEDVAKGGVPRLGAAVPRWYGETVAFWDGDVLITWTSNVQAWKSH
                     SSFEWSGKLQTVEIYTPIRNGGTFVGLNHEAIIYDEEALVEPIRIVRNLHKINNFTDA
                     KEEPYPFIECVQTIFSVAGHNSPVSPGDTIEYEIPDIYGRPWAQMWEKYFEEGMTKPD
                     PQEDLFNFN"
     gene            complement(1980..>3099)
                     /locus_tag="VM692_14000"
     CDS             complement(1980..>3099)
                     /locus_tag="VM692_14000"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUQ53335.1"
                     /translation="YTATQPLIVADLPIDGAQRRVVMQAPKNGFFYVLDAATGRLLSA
                     DKFAAVNWASGVDLATGRPIENPAARYEETGKPVALQPSSGGAHNWHPMAFSPLTGLV
                     YLSASNNALVYAPDRNFNPNPRVTNLGIDLAAGAQLGPDALADLPRGSFTLAWDPVAK
                     LEVWRAAGPAAGMLATAGGLVFGGKDRRLVAYDAADGRELWQSLDVQTGVVAGPISFE
                     LDGVQHIAVVAGRATGNYYAPVYSRLLVFRRGANLQLPPVREFTPPALNPPASVASAA
                     EIARGQDLYEANCVLCHDVVGNAGGLFRRGLFPDLAYSQALATRETFGAIVLDGARAA
                     NGMAGYAGVLDAETAEAVRAYIVDRANATLATQRGSGR"
BASE COUNT          511 a          994 c         1032 g          562 t
ORIGIN      
        1 agcagcgtcg cgctttccgg cggcatgctg gccgttggcg cacctggcga ggccagcagt
       61 gcgaagggta tcgccggcaa tcagaacgac aacagcgctg cttccagcgg agctgcctac
      121 gtattcgagc gcgcagcggg ggcgtggcaa caacgcgcct ccatcaaggc gtcgaacgcc
      181 gaggccggcg acggttttgg ttccgcggtc ggcctctcgc gcaattggct cgccgtcact
      241 gcgcccgagg aggacgcctc cgcgacggga gtaggcggag cgcagggcga taacggcaaa
      301 gctgacagtg gcgccacgta tctgtttgcc cgagatggct ctggtacctg gacgcaagag
      361 ctcttcatca agtcggcaaa ctccgacgcg aacgatcgat tcggtcgcac ggttgccttc
      421 agcccgggcg gcttgatcgt gggttcgccg ttcgaggata gtcacacgat ggccaatccc
      481 gcgagcaaca ctgcgtcggc cagcggcgcc gcctacgtgt tcgagtaacg ggcctgcgcc
      541 gacgctcagt tgaaattgaa cagatcttcc tgagggtcgg gcttcgtcat tccttcctcg
      601 aagtacttct cccacatctg cgcccacggg cggccgtaga tgtcaggaat ttcgtactcg
      661 atcgtgtcgc ccggcgacac gggcgagttg tggccggcga ccgagaagat cgtctggacg
      721 cactcgatga acgggtaggg ctcctctttc gcatccgtga agttgttgat cttgtgcagg
      781 ttgcgcacga tgcggatcgg ctcgacgagc gcttcctcgt cataaatgat cgcttcgtga
      841 ttgagaccga cgaaggtgcc gccgttgcgg atcggcgtgt agatctcgac ggtctgtagc
      901 tttcccgacc actcgaacga cgaatgtgac ttccatgctt ggacgttgct cgtccacgtg
      961 atcagcacgt cgccgtccca gaaagccacc gtctcgccgt accagcgtgg cacggccgcg
     1021 ccgaggcgcg ggacgccgcc tttcgcgacg tcctccatgt tgaactcgcg cccgacatgg
     1081 atgttcgtga tgaagttacg cgcaacgccc gccgtgatct gcacgaggtc cggcgtcgcg
     1141 atgatgtaat gctcccaaac ggagtacggg tagaagcgcc gcatgatccc ttcgggctga
     1201 cagaacgtcg acggccaata cgcgttgcca cgaacttggt ggtaagcctc ctgcaccgtg
     1261 cgctgctggt actcaggctt cagcaacgag agcagcgtcg agatctgcgt gtgcgcgccc
     1321 cagtaccacg tttgctgatt gcgcgggccc gcgacggggc gctcgtagac gccgttccac
     1381 tcgacggccg ggaaattctg gaacgtgtac ttctgcggac cgccgcggcc gcgcgtttcc
     1441 ttgagcagcg cctcgtagtg ctcttgcgcc gtcttgaagc cgtacgggct gacgatggcc
     1501 tcgcgcggta gcccaagctc gcagtgaccc cacggtccct gtttcgtatc gttcgtcagc
     1561 ggattggggc tcaagattcc gcgctgaatt tcggtggcca tcgggctgtt gcagcggaaa
     1621 tagcgcggat cgctccacag ctccttgtcg gcatagaagt cggtggtgtt gaagatgtcg
     1681 accggcaacg gtttgacgtt cgccggcagc tcgttcgggc ggaagccgtc gagcttcagg
     1741 ttcgcgttcg gctgaaacgg gttgccgatc cagccgggac ccacttcttt ctgatcgtgc
     1801 acgctcgcgg ccaacggccg cccgcggccg acgctcttga agccgatatc cggcatgtcc
     1861 tgcgccatga ccggcgccgc gctcaagatt gcggccagtg caaacgtgct ggttcgtgcg
     1921 ttcatacggt gcaatcctct aaagagattt caagtcgtcg caaagttcac gtgttggcgt
     1981 caacgccccg agcctcgttg cgtcgcaagt gtcgcattgg cgcgatcgac gatgtacgcg
     2041 cgcacggcct cggcagtctc tgcatcgagg acgccagcgt aacccgccat gccgttcgcg
     2101 gcgcgcgcgc cgtcgagcac gatcgcgccg aacgtctcgc gagtcgcgag cgcctgcgaa
     2161 taggcgagat ccgggaacaa gccgcgtcga aacaagccgc ctgcgttgcc gacgacgtcg
     2221 tggcacagca cgcagttcgc ctcatagagg tcttggccgc gcgcgatctc ggccgccgaa
     2281 gcgactgacg ccggcgggtt cagcgcgggc ggcgtgaact cgcgcacggg cggtagttgc
     2341 aggttggcgc cgcggcggaa cacgagcagc cgcgagtaca cgggcgcgta gtaattgccc
     2401 gttgcgcggc cggccacgac cgcgatgtgt tgcacgccgt cgagctcgaa gctgatcggt
     2461 cccgcgacta cgcccgtctg cacgtcgagc gactgccata gctcgcggcc atcggctgcg
     2521 tcgtacgcga cgaggcgccg gtccttgccg ccgaacacga ggccgccggc cgtcgcgagc
     2581 atgccggctg ccggcccagc cgcgcgccag acttcaagct tcgcgacggg gtcccacgcg
     2641 agcgtgaacg agccgcgcgg caggtcggcg agcgcgtccg gcccgagctg cgcgccggcc
     2701 gcgagatcga tgcctagatt cgtgacgcgc gggttcgggt tgaagttgcg atcgggcgca
     2761 tagacgagcg cgttgttgct ggccgacagg tagacgagcc ccgtcagcgg gctgaaggcc
     2821 atcggatgcc agttgtgcgc gccgcccgag gacggttgca gcgcgacggg cttgcccgtt
     2881 tcctcatacc gcgcggcggg gttctcgatc ggccggcccg ttgcaagatc gacgccgctc
     2941 gcccagttca cggccgcgaa cttgtcggcc gacagcaggc ggcccgtggc cgcgtcgagc
     3001 acgtaaaaga agccgttctt cggcgcctgc atgacgacgc ggcgctgtgc gccgtcgatc
     3061 ggcagatccg cgacgatgag cggctgcgtc gccgtgtag
//
LOCUS       DATLEE010000639         6356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3417978, whole genome shotgun sequence.
ACCESSION   DATLEE010000639 DATLEE010000000
VERSION     DATLEE010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3417978"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VM692_14005"
     CDS             <1..577
                     /locus_tag="VM692_14005"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HUQ53336.1"
                     /translation="VVALAAGAQRSSRGTDELVFESESMSFDLQNNLIQARRPRIRQG
                     NLHIVADEAVATGIEVDKASEWRLTGGVRIEVGTAVLEAASAVFTAANEQLSRGELVG
                     APVSFSDVDATQKTRITGRAQRMSYDYVAQTLRMYDASVQKGNTEILGCDLIYDFKAE
                     RVTSGSADCTDPFRARRIPGSEARPAPAAPQ"
     gene            574..1299
                     /gene="lptB"
                     /locus_tag="VM692_14010"
     CDS             574..1299
                     /gene="lptB"
                     /locus_tag="VM692_14010"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HUQ53337.1"
                     /translation="MSRLEALGVAKRYRLREVVKDLSLSIESGEVVGLLGPNGAGKTT
                     AFYMIVGLVRCDQGRIHLAGRDITKLPVHRRAKLGLGYLPQEASVFRKLSVADNIRAI
                     LETRRDLDRAARQTKLEELLEELHIGHVRDTLGVSLSGGERRRVEIARALAVEPKFVL
                     LDEPFAGVDPISVLDIQRIIRHLSQRDIGVLITDHNVRETLGICSRAYILNNGSLMAS
                     GSPDEILSNKSVREVYLGEGFSL"
     gene            1325..2776
                     /locus_tag="VM692_14015"
     CDS             1325..2776
                     /locus_tag="VM692_14015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HUQ53338.1"
                     /translation="MLKPSLQLKLSQQLTMTPQLQQAIRLLQLPVMELQTQIQTALDE
                     NVMLEVEESETAAEDGQAETASPDSDEPEDFSDGGAAEPEPEVAVAAETEWEDTQKTG
                     PSEAPKSADPRTTVEYADRSEETLRDHLLWQLELENLDARTTAIGQAIIDAINDDGYL
                     TDDIETIRATLAPDVLVSIEDIEQVLVKLVQQFDPVGIGARSVSECLQLQLCQLAADT
                     PGLELAKRIAAEHLPLVAEHQYGVLKRLLRVSDGDIEAAIGLVRACQPRPGANVFTAP
                     PEYVVPDVFVRRHEGQWVVELNNSMSPQLRVNQLYAGSLGRGEEYDALKAQLQEARWL
                     IRSLEIRNETLLKVALTIVQRQTEFLEQGEEFMRPMVLRDVAEAIEMHESTVSRVTTN
                     KYMHTPRGVFEFRYFFSSHVAGEEGDQSSTAVRAKIRKLVSAEDPEKPLSDSQIAQML
                     SEGGVTVARRTVAKYREAMKIPSSSDRKRSKSQ"
     gene            2796..3110
                     /gene="raiA"
                     /locus_tag="VM692_14020"
     CDS             2796..3110
                     /gene="raiA"
                     /locus_tag="VM692_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HUQ53339.1"
                     /translation="MQLSVTGHHIEITDSLRNYVGTKLARLERHFDHMTDIHCVLTVE
                     KLEHKAEATIHVGGGTLHAEQIEQNMYAAIDVLVDKLDRQVKKHKEKLTDHHAKEVAR
                     YR"
     gene            3149..3997
                     /gene="rapZ"
                     /locus_tag="VM692_14025"
     CDS             3149..3997
                     /gene="rapZ"
                     /locus_tag="VM692_14025"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HUQ53340.1"
                     /translation="MIISGVSGSGKSVALHLLEDLGFYCIDNIPVPLLRSFVDEIVPR
                     QDPAFENVGIGLDARSRPSDLAEVPALVQKLRGEGLSCEIIFLQTDTKVLLSRFSETR
                     RKHPLSDDKTSLEEAIAKERELLGPIINSAELVIDTTRMSVYALREQIRERVAPRTPG
                     SVSIMIESFGYKNGLPANADFVFDVRCLPNPYWEPQLRPLSGKDEPVKTFLDAAPMVQ
                     SMVDDITAFLEEWIPRYQDFQRSYLTVAIGCTGGMHRSVYVAEAVAKRLAPKHHSIRT
                     HHGEVR"
     gene            complement(3994..5163)
                     /locus_tag="VM692_14030"
     CDS             complement(3994..5163)
                     /locus_tag="VM692_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HUQ53341.1"
                     /translation="MLPGAGARGAYQVGVLKALASLLPERAPNPFSVICGTSAGAINA
                     AVLAGRAANFERAVADMEHVWGNFSASQVYRADDWTMLKTSAHWLAAGTLGGLGVRNP
                     ASLLDNSPLRELLAQNVRFDGIGRAIDQGHLDAVAITASAYATARSVTFFEGRPELAS
                     WTRIRRIGRATKLTVDHLMASSAVPFVFPPVQIGGEFYGDGSMRHRAPLSPAIHLGAD
                     RMLIVGVRDERPDAEPPAGAKPEPPTFAHLGGYMLDTLFMDGLYTDLERLSRTNRILE
                     QLGDKKLTGNLAALRPLHTLVIVPHQDLRSVAAQYVDELPRGVRLLLRGLGAGARSGM
                     QLVSYLLFESGFTRALIDMGYRDAMEMEDELRAFIFDEPMATLYAPAHLKLALDR"
     gene            complement(5184..>6356)
                     /gene="pyk"
                     /locus_tag="VM692_14035"
     CDS             complement(5184..>6356)
                     /gene="pyk"
                     /locus_tag="VM692_14035"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HUQ53342.1"
                     /translation="VAMDPHSGDQNAVGCAYRNLPKDVVAGDTLLLDDGLIVLEVEEV
                     SGTRIRSRVKQGGRLADHKGLNKQGGGLSAPALTDKDRRDIETAARLKLDFMSVSFAR
                     NAEDIEEARRLLRAAGGHAHIVAKIERAEAVTNIRAIIDASDVIMVARGDLAVEVGYA
                     SMTGLQKGLIRLARARNKVCITATQMLESMINSPSPTRAEVSDVANAVMDGTDAVMLS
                     AETAVGKYPVRAVRTMAELCIGAEKHETFRRAANYRLEDVFAHVDEAIAMAVMYVANH
                     LDVTAIIALTESGSTPLWMSRVRSDIPVFAFTRHEATLRRVSMYRGVYAVRFDEVLTD
                     DAVATQGRIFDSLLQGGSVSPGDLVLLTKGQSGVSGRTNSMLILTVPGASPPQASA"
BASE COUNT         1177 a         2066 c         2070 g         1043 t
ORIGIN      
        1 cgtcgtcgcg ctcgcggccg gcgcccagcg cagctcacgc ggcaccgacg agctcgtgtt
       61 cgagtccgag tcgatgagct tcgacttgca gaacaacctg attcaggcgc gccgccctag
      121 gatccggcag ggcaacctgc atatcgtggc cgacgaggcc gtggcgacgg ggatcgaggt
      181 cgacaaagcc agcgagtggc gcctcacggg cggcgtgcgc atcgaagtcg gaacggccgt
      241 tctcgaggct gcctcggcgg tcttcacggc ggccaacgag caattgtcgc gcggcgagct
      301 cgtcggcgcg cccgtctcgt tcagcgacgt cgacgcaacc cagaagacac gcatcacggg
      361 ccgggcgcag cggatgtcgt acgactacgt cgcgcaaacg ctgcggatgt acgacgcgtc
      421 ggtgcagaaa ggcaacaccg aaatcctggg ctgcgacttg atctacgact tcaaggcgga
      481 acgggtgacg tcgggctctg ccgattgcac cgacccgttc cgcgcgaggc gcattcccgg
      541 ttcggaagca cgccccgcgc ccgccgcgcc ccaatgagca ggctcgaagc gctcggagtg
      601 gccaagcgct atcggctgcg cgaggtcgtc aaagacctga gcctgtcgat tgagagcggc
      661 gaggtcgtgg ggttgctcgg cccgaacggc gccggcaaga ccacggcttt ctacatgatc
      721 gtcggcctcg tgcgctgcga ccagggccgg attcacctgg ccggccgcga catcacgaag
      781 ctgcccgtgc accggcgcgc gaagctcggc ctcggctatc tgccgcaaga ggcctcggtg
      841 tttaggaagt tgagcgttgc ggacaatatc cgcgctatcc ttgagacgcg gcgggacctc
      901 gaccgcgcgg ctcggcagac caagctcgaa gagctgctcg aagagctgca catcggccac
      961 gtccgggata cgctcggcgt gagcctctcg ggcggcgagc gccgtcgagt cgagatcgcg
     1021 cgcgctctcg ccgtggagcc gaagttcgtg ctgctcgacg agccgtttgc cggcgtcgat
     1081 ccgatatcgg tgctcgatat ccaacggatc attcggcatt tgagccagcg cgacatcggc
     1141 gtgctgatta cggatcataa tgtgcgcgag acactgggaa tttgcagccg cgcctatatt
     1201 ctgaacaacg gctcgctgat ggcttcaggc tcaccggacg agattctgtc gaataagagc
     1261 gtgcgggaag tgtatttagg agaaggtttc tccctctgag aagcggtccg gttagagtgt
     1321 ttgaatgctg aaaccatctt tgcaactgaa gctgtcgcaa cagctgacca tgacacccca
     1381 actgcagcag gccatccgcc tgctgcaact gcccgtcatg gagttgcaga cgcagataca
     1441 gacggcgctc gacgagaacg tcatgctcga agtcgaggag tccgagaccg ccgccgagga
     1501 cgggcaagcc gagacggcat cacccgattc cgacgagccc gaggacttct ccgatggcgg
     1561 cgcagccgag cccgaaccgg aggtcgcggt cgcagccgag accgagtggg aagacacgca
     1621 aaagaccggc ccatcggaag caccgaagag cgccgatccg cgtacgacgg tcgaatacgc
     1681 cgaccgctcc gaggagacgc tgcgcgatca ccttctgtgg cagctcgagc tcgagaatct
     1741 cgacgcgcgc acgacggcca tcggccaagc gatcatcgac gcgatcaacg acgacggcta
     1801 tttgacggac gacatcgaga cgatccgcgc gacgctcgct cccgacgtgc tggtgtcgat
     1861 cgaggacatc gagcaggtgc tcgtcaagct cgtgcagcag ttcgatcccg tgggcatcgg
     1921 agcgcgctcg gtcagcgagt gcctgcaatt gcagctctgt cagctcgctg ccgatacgcc
     1981 gggactcgag cttgcgaagc gcatcgcggc cgagcacctg ccgctcgttg cggagcacca
     2041 atacggagtg ttgaagcgct tgctgcgcgt ctcggacggc gacatcgaag cggccatcgg
     2101 gctcgtccga gcgtgccagc cgcggcccgg cgccaacgtg ttcacggcgc cgcccgagta
     2161 cgtagtgccc gatgtattcg tgcgccgtca cgagggccaa tgggtcgtcg agctcaacaa
     2221 ctcgatgtcg ccgcagttgc gcgtgaatca gctgtacgcg ggctcgctcg gccgcggcga
     2281 agagtatgat gctctgaagg cccagctcca agaggcgcgg tggttgattc gcagcttgga
     2341 aatccgcaac gagacgctgc tgaaggtcgc gttgacgatc gtccaacgcc agacggaatt
     2401 cctcgagcaa ggagaggaat tcatgcggcc gatggtgcta cgcgacgtag ccgaagccat
     2461 cgagatgcac gagtcgactg tctcgcgcgt cacgaccaac aaatacatgc acacgccgcg
     2521 cggcgtgttc gagtttcggt atttcttttc gagccatgtg gcgggcgaag aaggcgatca
     2581 atcgtcgacg gcggttcgcg ccaagatccg caagctcgtg agcgccgagg atcccgagaa
     2641 accattgagc gacagccaga tcgcgcagat gttgtccgag ggcggcgtca cggtggctcg
     2701 gcgcacggta gccaagtatc gggaggcaat gaagattcca tcatcgagtg atcggaagcg
     2761 gagtaagtcg caataaccag tggaaggagt gcgtcatgca gctgagcgtc acgggacatc
     2821 acatcgaaat caccgattcg ctgcgcaatt acgtcggaac gaagctcgcc aggctagagc
     2881 gccacttcga ccacatgacc gacatccact gcgtcctcac cgtcgagaaa ctcgagcaca
     2941 aagccgaagc cactattcac gtcggcggcg gcacgctcca cgcagagcag atcgagcaaa
     3001 acatgtacgc cgccatcgac gtcctcgtcg acaagctgga ccgccaagtc aagaaacata
     3061 aagagaagct caccgatcac catgccaaag aggtagcgcg ttatcgttga gcgcagtctc
     3121 gctatggagg gagcctcaat tgcggctcgt gatcatcagc ggcgtctcgg gctcgggcaa
     3181 gagcgtggcc ctccacttgc tcgaggacct cggcttctat tgcatcgata acattcccgt
     3241 cccgctgctg cgttcgttcg tcgacgagat cgtgccgcgc caggaccccg cgttcgagaa
     3301 cgtcggcatc ggcctcgacg cccgcagccg cccgagcgac ctcgccgagg tgccggcgct
     3361 cgtgcagaag ctccgaggcg aggggctctc gtgcgagatc atcttcctgc agacggacac
     3421 gaaggtgctg ttgtcgcgct tcagcgagac gcgccgcaag catccgctct ccgacgacaa
     3481 gacgagcctc gaggaagcca ttgccaagga gcgcgagctg ttgggcccga tcatcaactc
     3541 ggccgagctc gtgatcgaca cgacgcgcat gtcggtctac gcgctgcgcg agcaaattcg
     3601 cgagcgcgtc gctccgcgca cgccgggctc cgtgtcgatc atgatcgagt cgttcggcta
     3661 caagaacggg ctgccggcca atgccgactt cgtgttcgac gtgcgctgct taccgaaccc
     3721 gtactgggag ccgcagctgc ggccgctgag cggtaaggac gagccggtca agacgtttct
     3781 cgacgcggcg ccgatggtgc agagcatggt cgacgacatc acggcgtttc tcgaggaatg
     3841 gattccgcgc taccaggact ttcagcgctc gtacctgacc gtggcgatcg gctgcaccgg
     3901 cggcatgcac cgctcggtgt acgtcgccga agccgtcgcg aagcggttgg ccccgaaaca
     3961 ccacagcatc cgcacgcatc acggcgaggt gcgctagcga tcgagcgcaa gcttcagatg
     4021 tgccggcgcg tacagcgtcg ccatcggctc gtcgaagatg aaggcccgca gctcgtcctc
     4081 catttccatc gcatcgcgat agcccatgtc gatcagcgcg cgcgtgaagc cggactcgaa
     4141 cagcagatag ctgacgagct gcatgccgct gcgagcgccg gcgcccaagc cgcgcaacag
     4201 cagacgcacg ccgcgcggca actcgtcgac gtactgcgcc gcgaccgagc gcagatcctg
     4261 gtgcggcacg atgacgagcg tgtgcagcgg ccgcagcgcg gccaagttgc ccgtcagctt
     4321 cttgtcgccg agctgctcga ggatgcggtt ggtccgcgat aggcgctcga gatccgtgta
     4381 gaggccatcc atgaacagcg tgtcgagcat gtagccgccg agatgagcga acgtcggcgg
     4441 ctccggcttc gcgcccgcgg gcggctcggc gtcgggtcgc tcgtcgcgca cgccgacgat
     4501 cagcatgcgg tcggcgccga gatggatcgc cggcgacaac ggcgcgcgat gccgcatcga
     4561 gccgtcgcca tagaactcgc cgccgatctg cacgggcggg aacacgaatg gcacggcgga
     4621 gctcgccatc aagtgatcga ccgtcagctt cgtcgcgcgg ccgatgcggc ggatccgcgt
     4681 ccacgaagcg agctccggcc gcccttcgaa gaacgtcacg gagcgagccg tcgcgtacgc
     4741 cgaggccgtg atcgccacgg cgtcgagatg gccctgatcg atcgcgcggc cgatgccgtc
     4801 gaagcgcacg ttttgggcca gcagctctcg tagcggcgag ttgtcgagca gcgatgccgg
     4861 attgcggacg ccgagcccgc ccagcgtacc ggccgcgagc cagtgcgcgc tcgtcttcaa
     4921 catcgtccag tcgtcggcgc gatagacctg gctcgccgag aaattacccc agacgtgctc
     4981 catgtcggcg acggctcgct cgaaatttgc cgcgcgcccc gcgagcacgg cggcattgat
     5041 tgcaccggcc gacgtgccgc agatgacgct gaacgggttc ggcgctcgct cgggcagtag
     5101 gctcgccagc gctttcagta ctccgacttg ataggcgccg cgggcccccg cgcccggcag
     5161 cacgagtgct ttgcgagtac ttctcaggca ctcgcctgcg gcggcgatgc gcccggcacc
     5221 gtgagaatca gcatcgagtt cgtgcggccc gacactccag actgcccctt cgtcagcaac
     5281 accagatcgc cggggctcac ggagccgccc tgcaacagcg agtcgaagat gcggccctgc
     5341 gtggcgactg cgtcgtccgt caagacctcg tcgaaccgca cggcatagac gccgcggtac
     5401 atgctgacgc ggcgcagcgt cgcttcgtgg cgcgtgaacg cgaacacggg gatgtccgag
     5461 cgcacgcgcg acatccacaa cggcgtcgag cccgactcgg tcaaggcgat gatcgcggtg
     5521 acgtcgagat ggttcgcgac gtacatgacg gccattgcga tcgcttcgtc gacatgcgcg
     5581 aacacgtcct cgagccggta gttcgcggcg cggcggaacg tctcgtgctt ctcggcgccg
     5641 atgcagagct ccgccatcgt gcgcacggcg cgtacgggat acttaccgac ggccgtctcg
     5701 gccgacagca tcacggcgtc cgtgccgtcc atgacggcgt tcgcgacgtc cgacacttcg
     5761 gcgcgcgtcg gcgacggcga gttgatcatc gactcgagca tctgcgtcgc cgtgatgcag
     5821 actttgttac gcgcgcgcgc gaggcggatc aggccttttt gcaaacccgt catcgacgcg
     5881 tagccgacct cgacggcgag atcgccgcgc gcgaccatga tcacgtcgct ggcgtcgatg
     5941 atcgcgcgga tgttggtcac ggcttcggcg cgctcgatct tggcgacgat gtgtgcgtgg
     6001 ccgccggccg cgcgcagcag ccgacgcgct tcttcgatgt cctctgcgtt gcgcgcgaac
     6061 gataccgaca tgaaatcgag cttcagccgc gcggccgttt cgatatcgcg ccggtccttg
     6121 tccgtcagcg ccggggccga gaggccgccg ccctgcttgt tcaaaccttt gtgatcggcc
     6181 agccggccgc cttgcttcac tcgagaccga atgcgcgtgc cgctgacttc ctcgacctcg
     6241 agcacgatga gcccgtcgtc gagcagcagc gtgtcccccg caacgacgtc tttcggcaga
     6301 ttgcggtagg cgcagccgac ggcgttctgg tcgccgctgt gcggatccat cgcgac
//
LOCUS       DATLEE010000640         3722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3418244, whole genome shotgun sequence.
ACCESSION   DATLEE010000640 DATLEE010000000
VERSION     DATLEE010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3418244"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VM692_14040"
     CDS             complement(<1..281)
                     /locus_tag="VM692_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ53343.1"
                     /translation="MRILVIAPQPFYQERGTPIATRLLIEALQTAGHEVDVLTYHVGD
                     DPGLRGVRVFRSPAVPFVRDVPIGFSVRKLLCDLALGWRLLTLTRQRRY"
     gene            complement(286..1014)
                     /locus_tag="VM692_14045"
     CDS             complement(286..1014)
                     /locus_tag="VM692_14045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ53344.1"
                     /translation="MISAAARETPDIETASDSYARRFAGAAGRYLLAEQDAAVRAALG
                     DWRGGTVLDVGGGHAQLTPLLRTVATEVLVFGSDARSLERVRRDFPDCATATGDLLDM
                     PFATRSFDVVVAVRLLPHVRNWQRLLTELCRVARSTVIVDYPRISGFNGLTPLLFPLK
                     KRLEGNTRHYRNFRDAELDDVLRGSGFVPRQRHVQFLLPMVVHRRANGARALRRVEEL
                     AKSLHLTAALGSPVVLRADRAPEV"
     gene            complement(1031..2887)
                     /locus_tag="VM692_14050"
     CDS             complement(1031..2887)
                     /locus_tag="VM692_14050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUQ53345.1"
                     /translation="MRAILEKGIPELPSGMVYLRGGIFSHLMAASASFFGFSEFALRL
                     PAALFGIALIPAAYVFGNALFGRVVGLVTAALLAVSFWDIDLSRYARFYSAFSVLYVL
                     TLYAIWQFRIKRPSAAGGVLCVALAIVTLSLHDLAYSLALAFFYPLVLRGSAAWRSPR
                     EWLWPIAASGVLAAFYVGWREYVLRARALPALADSAPGAAGGTELDELGRSFVALPDM
                     PLLTGMLDRLPLFVPLLVLAAVACTWLVTRRLGSPARERVLLAVIAACAAAQLFNLAL
                     LGTFALAFMKRTGLPALRSREVLLAGAVIAATFVIWVAAALFAQLDLGPLQPLTPRTL
                     VRELLNFPYFYVFWGYPNEWPLATAVASIGALVAFHRAARAKDAAAGFALLALSGPIV
                     GNALFSSPFELFRYNAAFNTLFFVFVALAFVHWRELVPAWRPALPRPLLGRTAVGLGT
                     TLLVLLVTAYDLNPLRGWLAVERQYVNDGVLYRTFGITAYDDFKTTAAFVAQNAAPHD
                     LIVTPDSREYYNYLGRVDYWLRSGRYDDQSYKTDGTLRDLYLGTPLVKTLPELEAALA
                     LPNRTKWVLASSANLKDPRTPVAPEIRNFLRNAEQRVVYVGLDRDRKVYRFD"
     gene            3291..>3722
                     /locus_tag="VM692_14055"
     CDS             3291..>3722
                     /locus_tag="VM692_14055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ53346.1"
                     /translation="MTDVSIVVPLYNEQDNVARVCTATHEALARMRRSYEIVLVDDGS
                     SDGTYAAAAAIARIDPRVRFVVLARNSGQTAAMAAGMANARGKYLVTMDGDLQNDPRD
                     IPLLIDTLEAGYDLVAGWRMKRQDKLVTRKIPSRIANWLIGK"
BASE COUNT          690 a         1233 c         1305 g          494 t
ORIGIN      
        1 tcgtagcgcc gttggcgcgt cagcgtcagc agccgccatc cgagcgcgag gtcgcagagc
       61 agcttacgca ccgagaagcc gatcggcacg tcgcgcacga acggcacggc cggcgagcga
      121 aacacacgca cgccgcgcag gccggggtcg tcgccgacgt gataggtcag cacgtcgacc
      181 tcgtggccgg ccgtctgcag cgcttcgatc agcaagcgcg tcgcgatcgg cgtgccgcgc
      241 tcttgataga aaggctgtgg ggcgatgact agaatccgca tgcgcctaaa cctcgggcgc
      301 gcggtcggcg cgcaatacga cgggggagcc gagcgcggcc gtgaggtgca gcgacttcgc
      361 gagctcctcg acgcggcgca aggcgcgggc gccgttggcg cggcggtgca cgaccatcgg
      421 cagcaggaac tgcacgtggc gttgccgggg cacgaagccg cttccgcgca gcacgtcgtc
      481 gagctcggcg tcgcggaaat tgcggtagtg gcgcgtgttg ccctcgagcc gcttcttcag
      541 cgggaacagg agcggcgtca agccgttgaa ccccgagatg cgcggatagt cgacgatgac
      601 ggtcgagcgc gcgacgcggc agagctccgt cagcagacgt tgccagttgc gcacgtgagg
      661 caacaggcgc accgcgacca cgacgtcgaa gctgcgcgtc gcgaacggca tgtcgagcaa
      721 gtcgccggtc gccgtcgcgc agtccgggaa gtcgcgacgc acgcgctcga ggctgcgcgc
      781 gtcgctgccg aacacgagca cctcggtcgc cacggtgcgc agcagcggcg tcagctgcgc
      841 gtgaccgccg ccgacgtcga gcacggtgcc gccgcgccaa tcgccgagcg cggcgcgcac
      901 ggcggcatcc tgctccgcga gcaggtaccg gccggcggcg ccggcgaagc gccgtgcgta
      961 ggaatccgac gccgtctcga tgtcgggggt ttcgcgggcg gctgcgctga tcatgctggt
     1021 cgggtctccg tcaatcgaag cgatagactt tgcggtcgcg gtcgaggccg acgtacacga
     1081 cgcgctgctc ggcgttgcgc aggaaattgc gaatctcggg cgccaccggc gtgcgcgggt
     1141 ccttgagatt cgcgctcgac gccagcaccc acttcgtgcg gttcggcagg gccagcgcgg
     1201 cttcgagctc cggcaacgtc ttgacgagcg gcgtgccgag atagaggtcg cgcaacgtgc
     1261 cgtcggtttt gtagctctgg tcgtcgtagc ggccgctgcg cagccaataa tcgacgcggc
     1321 cgaggtagtt gtagtactcg cgcgagtccg gcgtgacgat gaggtcgtgc ggtgccgcgt
     1381 tctgcgcgac gaacgccgcg gtcgtcttga agtcgtcgta ggccgtgatg ccgaacgtgc
     1441 ggtagagcac gccgtcgttg acgtactgcc gctcgacggc gagccagccg cgcaacgggt
     1501 ttaagtcgta ggccgtgacg agcagcacga gcagcgtcgt gccgagcccg accgccgtgc
     1561 ggccgagcag cggccgcggc aaggcggggc gccaggccgg cacgagctcg cgccagtgca
     1621 cgaacgcgag cgcgacgaac acgaagaaca gcgtgttgaa cgccgcgttg tagcggaaca
     1681 gctcgaacgg cgacgagaac agcgcgttgc cgacgatcgg gcctgacagc gccagcagcg
     1741 cgaaccccgc ggccgcgtcc ttggcgcgtg ccgcgcggtg aaacgccacg agggcgccga
     1801 tcgacgctac ggccgtcgcg agcggccact cgttgggata gccccagaat acatagaagt
     1861 acgggaagtt caggagctcg cggacgagcg tgcgcggcgt cagcggctgc agcggtccca
     1921 gatcgagctg cgcgaacagt gcggccgcga cccagatcac gaacgtcgcc gcgatcacgg
     1981 cgccggcgag caggacctcg cggctgcgca gcgccggcag tcccgtgcgc ttcatgaacg
     2041 cgagcgcgaa cgtgccgagc agcgcgagat tgaagagctg agccgccgcg cacgccgcga
     2101 tgactgccag caagacgcgc tcgcgcgccg ggctgccgag ccggcgcgtg acgagccaag
     2161 tgcaggcgac agccgcgagc acgagcagcg gcacgaatag cggcaggcgg tcgagcatgc
     2221 cggtcagcag cggcatgtcg ggcagcgcga cgaacgagcg accgagctcg tcgagctctg
     2281 tgccgccggc ggcgccgggc gcgctgtcgg cgagcgcggg cagcgcgcgt gcgcgcagca
     2341 cgtattcgcg ccagcccaca tagaacgcgg cgagcacgcc gctcgctgcg atcggccaca
     2401 gccactcgcg cggcgagcgc cacgcggccg agccgcgcag cacgagcgga tagaaaaacg
     2461 ccaacgccag cgaatacgcg aggtcgtgca gcgacagcgt cacgatcgcc agcgcgacgc
     2521 acaacacgcc gccggcggcg ctcggtctct tgatgcgaaa ctgccagatc gcgtacagcg
     2581 tcagcacgta cagcacgctg aaggccgagt agaagcgcgc gtagcgcgag aggtcgatgt
     2641 cccagaacga gacggcgagc aatgccgctg tcacgagccc gacgacgcgg ccgaacaatg
     2701 cgttcccgaa aacataggcg gcgggaatca gcgcgatgcc gaacagcgcc gccggtaagc
     2761 gcaatgcaaa ttcgctaaag ccgaagaacg acgcgctggc cgccatcaga tgactgaaaa
     2821 tgcccccgcg tagataaacc atgccgctcg gaagctcggg gattcctttc tcgagaatcg
     2881 cgcgcaccgc taaccccgag agatcttcat cccaacgaaa tccgagtgcg cccaaatgcg
     2941 tgagacgtag ataagcacct gtcgcgatga tcgcggcgag cgcccaccac tcgcgcgccc
     3001 aactaacgtg gctcgcgtca acgcgtcgag ctccccaact caacttcact tcgtcacccc
     3061 agcgagatgc cgttcgagcc atcgctcgcc ccggcgttgt tgcttggcat tgcctttgca
     3121 agaaccaggc cgacgaaggc ggtagaggtt gcgcgaagcc cacagccgcg aggcacgcgc
     3181 cgcgagggtg caaggcacgc acgcaaccga tcccgtcctg cgcaagggag gttttgagtt
     3241 ttgcaatacg gaatttgcaa agccggggcg ggctctcgct cgggcaagcc atgaccgacg
     3301 tatcgatcgt cgtcccgctg tacaacgagc aagacaacgt cgcgcgcgtg tgcacggcca
     3361 cgcacgaggc gctcgcgcgc atgcggcgca gctacgagat cgtcctcgtc gacgacggca
     3421 gcagcgacgg cacttatgcc gcggccgccg cgatcgcgag gatcgatccg cgtgtccgat
     3481 tcgtcgtgct cgcgcgcaac tcggggcaga ccgccgcgat ggcggccggc atggcgaacg
     3541 cgcgcggtaa gtatctcgtc acgatggacg gcgacttgca gaacgatccg cgcgacatcc
     3601 cgctgctgat cgacactctc gaggcggggt acgacctcgt cgcgggatgg cgtatgaagc
     3661 ggcaggacaa gctcgtcacg cgcaagattc catcgcgcat cgccaattgg cttatcggca
     3721 ag
//
LOCUS       DATLEE010000641         2676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7127399, whole genome shotgun sequence.
ACCESSION   DATLEE010000641 DATLEE010000000
VERSION     DATLEE010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7127399"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VM692_14060"
     CDS             <1..683
                     /locus_tag="VM692_14060"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HUQ53347.1"
                     /translation="RFYSSSSFGDLVDILMLDGRQYRSSQACQPGQLVVPCPELYAGD
                     RTMLGDAQERWLSTQLGASTARWTLFGQQTLFTHFDQSGDGPLAYWADAWNGYPAARE
                     RFLATLAQRRTSNPVILGGDIHAFVVGDVNAMPENPESPIVATEFVATSISSVGAPQA
                     NFDRWLKENPNVRLGRSDQRGYLRVTVEPEHLRADLMAVDDVTREDSPSHVLASFDVA
                     SGRPGVAR"
     gene            723..2105
                     /locus_tag="VM692_14065"
     CDS             723..2105
                     /locus_tag="VM692_14065"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUQ53348.1"
                     /translation="MTIPVARRPHSRLLAPLFAAAALACAMTPLSFSQESTEVKPIIP
                     YPTQTRTLPNGLTVVVVPMPSDGLVAYWTIVRTGSRDEYEKGRSGFAHFFEHMMFRGT
                     ERFPSDVYNRIITTIGADANAYTTDDHTAYHLSMAAEDLEQVMELESDRFQRLSYAEP
                     AFQTEAGAVYGEYRKTRMEPEFALYEELVATAFEHHPYGHTTMGYERDIAIMPTLYEY
                     SRTFFTRYYRPENTVLFIAGDVTPANVFALVEKHYGGWQRGYVAPQIPVEPEQKAERR
                     IDVKYDGQTLPILQLAYKLPLYDPADRTRVAADLFADLAFGETSDAYRQLVLDEQVVE
                     DLDAGAAYNRDPGLLEISARIKDPAKIDYVLGVIDATIAEFRANEPDAQRLADLQRRL
                     HYGFLMGLQTPDSLAARVAPFLALHGNLQGLDQLYATYAAVTPADVHAAAERYLHEER
                     RTIGVLTSRQ"
     gene            2204..>2676
                     /locus_tag="VM692_14070"
     CDS             2204..>2676
                     /locus_tag="VM692_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HUQ53349.1"
                     /translation="MVLLSVPADPTISLSLQFAVGSQNDPPGKEGLAFLTGEMLSDAA
                     TEARSLDEILAALYPLAASYGMRVDIERSTLTGRVHRDNLAAYLELFTDAFVKPAFDA
                     NDFERVRNDTVNAIENALRYSSDEELGKAALHDFVFRDTPYAHLSEGTVAGLKSIT"
BASE COUNT          476 a          932 c          826 g          442 t
ORIGIN      
        1 gacgctttta ttcgagcagc agcttcggcg acctcgtcga tatcttgatg ctcgacggtc
       61 ggcagtaccg ctcgtcacaa gcgtgccaac caggtcagct cgtggtgccc tgccccgagc
      121 tctatgccgg tgaccgcacg atgctcggcg acgcacagga gcggtggctc tcgacgcagc
      181 tcggtgcgag caccgcgcgc tggacgctgt tcggccagca aacgcttttc acgcacttcg
      241 atcaaagcgg cgacggccct ctggcttatt gggccgacgc gtggaacggc tatcccgcgg
      301 cgagagagcg cttcctcgcg acgctcgccc aacgcaggac gagcaatccc gtgatcctgg
      361 gcggcgacat ccacgcgttc gtcgtcggcg acgtcaatgc gatgcccgaa aacccggagt
      421 ctcccatcgt cgccacggaa ttcgtcgcga cctcgatcag ctcggtcggc gcgccgcagg
      481 ccaatttcga tcgctggctc aaagagaatc cgaacgtgcg gctcgggcgc agcgaccagc
      541 gtgggtatct gcgcgtgacg gtcgagcccg agcacctgcg cgccgacctc atggccgtcg
      601 acgacgtcac gcgcgaagac tcgccgagcc acgtgctcgc gtcgttcgac gtggcgagcg
      661 gcagaccggg ggtcgcgcgc tagccacgcg gcgttgcgta cttcggccgg tataattccg
      721 ggatgacgat tcctgtcgca cgccggccgc acagtcggct gttggcgccg ctgttcgcag
      781 cggcagcgct ggcgtgtgcg atgactccac tgtccttcag ccaagagagc accgaagtga
      841 aacccatcat tccttatccc acgcagacgc ggacgttgcc gaacggcttg accgtcgtcg
      901 tcgtgccaat gccaagcgac ggcctcgtcg cctactggac catcgtgcgt acaggctcgc
      961 gcgacgagta cgagaaaggg cggtcgggct tcgcgcactt cttcgagcac atgatgtttc
     1021 gcggcaccga gcgtttcccg tccgacgtgt acaaccgcat catcactacg atcggcgccg
     1081 acgcgaacgc gtacacgact gacgatcaca cggcatatca cctgtcgatg gcggccgagg
     1141 atctcgagca agtcatggag ctcgagagcg accgcttcca gcgcctgtcg tacgccgagc
     1201 ccgcgttcca aacggaggca ggcgccgtct acggcgagta ccgcaagacg cgcatggagc
     1261 ccgagttcgc gctctacgag gagctcgtag cgacagcgtt cgagcaccat ccgtatggcc
     1321 acacgacgat gggctatgag cgcgacatcg cgatcatgcc gacgctctac gagtattcgc
     1381 gcacgttctt cacccgctac taccggcccg agaacaccgt gctgttcatt gccggcgacg
     1441 tgacgccggc gaacgtgttt gcgctcgtcg agaagcacta cggcggctgg cagcgcggct
     1501 acgtggcgcc acagattccc gtcgagcccg aacagaaagc cgagcgccgc atcgacgtca
     1561 agtacgacgg ccagacgctg ccgatcctgc aactcgccta caagctgccg ctctacgatc
     1621 cggcggaccg cacacgcgtc gccgctgact tgttcgccga tctcgcgttc ggcgagacga
     1681 gcgacgcgta ccgccagctc gtgctcgacg agcaagtcgt cgaggacctc gacgccggcg
     1741 cggcgtacaa ccgcgacccc ggactgctcg agatctcggc gcgcatcaag gatcccgcca
     1801 agatcgacta cgtgctcggc gtcatcgacg cgacgatcgc ggagtttcgc gccaacgagc
     1861 ccgacgcaca gcggctcgcc gatctgcagc ggcggctgca ctacgggttc ttgatggggc
     1921 tgcagacgcc ggactccctc gcggctcgag tcgccccgtt cctggcgctg cacggcaatt
     1981 tgcaagggct cgatcagctc tatgcgacct atgccgccgt cacgccggcc gacgtgcacg
     2041 ccgccgccga acgctatctg cacgaagagc gccgcaccat cggcgtgctg acgtcgcggc
     2101 aatgacgcga gcggcgcgca tcgccgtctt cgttctcgcc gcactgtgcg cgagctgtga
     2161 aagcgcaccg ccctccgccg ccttaaaccc cgaggaaccc aacgtggtac tgctcagcgt
     2221 ccctgccgac ccaacgatct cgctatcgct gcaatttgcc gtcggctcgc agaacgatcc
     2281 gcccggcaag gagggcctcg ctttcctgac tggcgagatg ctgtccgacg ccgccaccga
     2341 ggcgcgctcg ctcgacgaga tccttgcggc cctctacccg ctcgcggcga gctacggcat
     2401 gcgagtcgac atcgagcgct cgacgctgac gggccgcgtg caccgcgaca atctcgcggc
     2461 gtatctcgag ctcttcaccg acgctttcgt gaagccggcg ttcgacgcga acgacttcga
     2521 gcgcgtgcgc aacgacaccg tcaacgcgat cgagaatgcg ctgcgctact cgtccgacga
     2581 ggagctcggc aaggcggcac tgcacgactt cgtctttcgc gacacgccgt acgcgcactt
     2641 gagcgaaggc acggttgcag ggctcaagtc gataac
//
LOCUS       DATLEE010000642         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7844024, whole genome shotgun sequence.
ACCESSION   DATLEE010000642 DATLEE010000000
VERSION     DATLEE010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7844024"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..666
                     /gene="queE"
                     /locus_tag="VM692_14075"
     CDS             1..666
                     /gene="queE"
                     /locus_tag="VM692_14075"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017165275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HUQ53350.1"
                     /translation="MAAAAELESAAPRLKITEIFFSIQGEAAFSGWPTVFVRLTGCPL
                     RCQYCDTAYAFTGGEWRTFAEITTEIRRHETPYVCVTGGEPLAQPRCGALLTELCDAG
                     FDVSLETSGAIDISPVDARVARVVDLKTPGSKEEHRNRWQNLELLTPRDAVKIVICDR
                     SDYEWARDVVAQRSWPCPVFFSPSHEQLPAGQLADWILEDRVPARLQVQLHKVLWGTV
                     PGR"
     gene            671..1339
                     /gene="queC"
                     /locus_tag="VM692_14080"
     CDS             671..1339
                     /gene="queC"
                     /locus_tag="VM692_14080"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HUQ53351.1"
                     /translation="MSRSVVLLSGGLDSATALGIARARGDECHTLAFDYGQRHRSELV
                     ASRRVSASLRAAAHREMRVSLADFGGSALTDRNIAVPEQPTTGIPPTYVPARNTVFLS
                     LAMALAEVIAADRIVIGVNFLDSSGYPDCRPEFIAAFQQLARLATKRGVEGGALTIDA
                     PLINLTKADIIRRGTELGVDYSLTVSCYQADAEGRACGRCDSCRLRREGFAAAQLPDL
                     TRYG"
     gene            complement(1336..>2066)
                     /locus_tag="VM692_14085"
     CDS             complement(1336..>2066)
                     /locus_tag="VM692_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53352.1"
                     /translation="QCQIALTSTSIPVDVPVPTELPATRDDGNEKIIWRLEAAGECPG
                     PDFWSSFELPVFATAETPQPAAMPPAPTGSERPQARALAALGIDYERTPQGGEAWTFR
                     RGRHKGVALAISAFAVVWSGACVALFASEAPLLMAIVFALFDVLAVWWALHLWLTEYR
                     VTLNGNVLTLVRRGFMARAPIEIPRAWLRGVRAKRGMQAGNKLYYDLEVESADGKFTA
                     ASSLADYDVANWLARYWMQGEARS"
BASE COUNT          335 a          700 c          691 g          340 t
ORIGIN      
        1 atggccgcgg cggcggaact ggaatctgca gccccgcggc tgaagatcac ggagatcttc
       61 ttttcgatcc aaggcgaggc cgcgttcagc ggctggccga ccgtgttcgt gcggctgacg
      121 ggttgcccgc tgcgttgtca gtactgcgac accgcgtacg cgttcacggg cggcgagtgg
      181 cgcacgttcg ccgagatcac gaccgagatc cgccgccacg aaacgccgta cgtctgcgtt
      241 acgggcggcg agccgctcgc gcagccgcgc tgcggcgcgt tgctgacgga gctctgcgac
      301 gcaggattcg acgtgtcgct cgaaacgagc ggcgcgatcg acatttcgcc cgtcgacgct
      361 cgcgtcgcgc gcgtcgtcga cctcaagacg ccggggtcga aggaagagca ccgcaatcgc
      421 tggcagaacc tcgagctgct gacgccgcgc gacgccgtca agatcgtgat ttgcgatcgc
      481 tccgactacg agtgggcgcg cgacgtcgtc gctcagcggt cgtggccgtg ccccgtgttc
      541 ttctcgccta gccacgagca gctgccggcg gggcagctcg ccgactggat cctcgaggac
      601 cgtgtgcccg cgcgcttaca agtgcagctg cacaaggtgc tctggggcac cgttccgggg
      661 cggtaattta atgagtcgct ccgtcgtgct gctttccggt ggactcgatt cggcgacggc
      721 gttgggcata gcgcgcgccc gaggcgacga gtgccacacc ctcgcgttcg actacgggca
      781 gcggcatcgc tcggagctcg tagcatcgag gagggtatcg gcttcgctgc gcgccgcggc
      841 acatcgcgag atgcgcgtgt cgctcgccga cttcggcggc tcggcgctca cggacagaaa
      901 catcgcggtc cccgagcagc cgacgacggg catcccccca acctacgtcc cggcacgcaa
      961 caccgtgttt ctaagcttgg cgatggcttt ggccgaggtc atcgcggccg accgcatcgt
     1021 catcggcgtg aatttcctcg acagctccgg gtatccggac tgccgccccg aattcatcgc
     1081 ggcatttcag cagctcgcaa ggctcgcgac gaagcgtggc gtcgagggcg gagcgctgac
     1141 gatcgatgcg ccgctgatca acctgacgaa agccgacatc atccgccgcg gcaccgagct
     1201 cggcgtcgac tactcgctga ccgtgtcgtg ctatcaagcc gacgccgaag gccgcgcctg
     1261 cggccgctgc gattcgtgcc gattgcgccg cgaaggtttc gcggcggcgc aactgccgga
     1321 ccttacgcgc tacggttagc tgcgcgcttc gccctgcatc cagtaacgtg cgagccaatt
     1381 cgcgacatcg tagtccgcga gtgagctcgc ggccgtgaac ttgccgtcgg cggattcgac
     1441 ctcgagatcg tagtaaagct tgttgccggc ctgcattccg cgcttggcgc ggacgccgcg
     1501 cagccaagcg cgcggaatct cgatcggcgc gcgcgccatg aagccgcggc gcacgagcgt
     1561 caacacgttg ccgttcaagg tcacgcggta ttccgtgagc cacaagtgca atgcccacca
     1621 gaccgcgagg acgtcgaaca gcgcgaacac gatcgccatt aacagcggcg cctcggacgc
     1681 aaacagtgcg acgcaggcgc cggaccagac gacggcgaac gcgctgatcg cgagcgcgac
     1741 gcctttgtgc cggccgcgac ggaacgtcca cgcctcgccg ccttgcggcg tgcgctcgta
     1801 gtcgatgccg agcgccgcga gcgctcgcgc ttgcggccgt tcgcttccgg tcggcgcggg
     1861 aggcatcgca gcgggctgcg gcgtctccgc cgtcgcgaag acaggcaact cgaagctgct
     1921 ccagaaatcg gggcccgggc attcgccggc ggcttcgaga cgccaaatga tcttctcgtt
     1981 gccgtcgtcc ctcgtcgccg gcagctcggt cggcacgggg acgtcgacgg gaatcgacgt
     2041 cgacgtcaaa gcgatctgac actgat
//
LOCUS       DATLEE010000643         6086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3421501, whole genome shotgun sequence.
ACCESSION   DATLEE010000643 DATLEE010000000
VERSION     DATLEE010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3421501"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2475
                     /locus_tag="VM692_14090"
     CDS             <1..2475
                     /locus_tag="VM692_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ53353.1"
                     /translation="QAAVDSIFLANPTQYAGYFPCNGWNTETDGVAGITFADCARGAA
                     NAGGGRWAVNRTSEIYFNRDGSPFVLAGAHGYNSGFGSTAIGDGYAGMRLQPNGDLGQ
                     VAFDGQAATPLERRSLFGRATHEINDHLTAFAQATYSGVNVSTTGGYPPAITVWSAPV
                     PLDGRPIPAALQTLLASRVRNVDPDGAGPAVAVNQGPTDPWQLFRVLDFLGGAVTTDV
                     DRDVYQIMAGVQGSFSQRDWTWEAYVSSGQTSESDFFSNMPSLQRYWNLIGAAPNALT
                     LLGAPGNGTWGRGTFTQGRNYAQTCTSGLPIFANSSNLGPGQVSQDCLESIAANTRSI
                     TELSQEIAEFNLQGKIVDMKAGELRFAVGVSSRKNEFSFEPGETNDRESVAEQPMSIF
                     ASNDTAGGTKVQELYGELLVPVTNKFDLEFGVRASDYVDSTVGTTQTSKAMFTFRATD
                     ALTLRGGYQKAERAANTAELFQGVSLLVVPFGPSDPCSFTTTAPWGNVASNPNRLAVQ
                     SLCAAIINNSDGITANDNTSAFGLPGSGTANNFARPGNPFFPLEIELRKGNPAVQPEK
                     GETFTLGLVVQGPGSLENLTASFDWYDIEITDAIAPLNSLFAHQQCFNANGSSNPTLS
                     YTGNPYCALILRNVVSGERSSVDAPFINTGSLKTSGMDITVNWTTDVGDGGSFFINSL
                     LTVLDEYIVQDSPTAIPFDAKGTLDQGGQYDWKMTNTFGYNFSGGKANVGLQWRYLPE
                     IKDETFARTPNTRVFPVDSYQTLNLFAGYSLNEKINLRMGIDNLLDEDPLIVGAQPGD
                     NNAEVTRADYFDILGRRAYVGVKMSF"
     gene            complement(2540..3091)
                     /locus_tag="VM692_14095"
     CDS             complement(2540..3091)
                     /locus_tag="VM692_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53354.1"
                     /translation="MNSRKFNARTAACALLLASAITGGANAQDGKDRDDDEATVAKAC
                     LDHNTIRRTRILGDRNIAFVTKRNEIYNNQLPRQCPSLNRNSLVNYAVANRELCAGSI
                     FQVLWQTGLGNYTPAFTCQLGAFVPITEDELETLAAVNNEDSDRRRPRRRSSQEAVTT
                     EQVELPPRAEAAPEAATPPAPQQ"
     gene            3219..4820
                     /locus_tag="VM692_14100"
     CDS             3219..4820
                     /locus_tag="VM692_14100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUQ53355.1"
                     /translation="MSLPPGVSERDFERAVARFRSVVGADWVFTSDEDTALYRDAYSP
                     FFNEPEEKVASAAVAPETAEHVVEIVRIANELRIPLYPISTGRNLGYGGSAPVLSGSV
                     VLDLKRMNRILEVDERNAYALVEPGVSYFDLYRHIEERGLKLWIDCPDPGWGSVIGNA
                     LDHGVGYTMNPYRDHWGAQCGLEVVTGDGELVRTGMGALPKSRTWQQFKYGFGPYLDG
                     MFSQSNFGVVTKMGVWLMPQPEAYVDCNIDVFGHDDIMPLVDHMNYVMNTGLQNAGTN
                     LQSPIRFAVAGNPELRAALASEDPAEIAKLNAFAREREVPFYSNVFKFYGPEEVVRAQ
                     LEYTRRRLLTIPGARFREGAFYKFPITAEQKANVRDPQDLGIPTLETFSIGARSNFAP
                     ATRGHLGFSPVIPMRGEDVIESQRVFQRVLQEFGTDVALLVFPQSYHPRTFVILCMFY
                     VMPDPAHNDAVRRTFKRLVEVAAEHGWGEYRTHTAFMDDCAAAYSFNDHALKKLQEKI
                     KDAVDPNGVLSAGRYGIWPKHLRKS"
     gene            complement(4869..>6086)
                     /locus_tag="VM692_14105"
     CDS             complement(4869..>6086)
                     /locus_tag="VM692_14105"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ53356.1"
                     /translation="PRFAFLYIANGVIQDQWTPATTGGGYELTPILKPLAAHKADINV
                     LSGLSHLQADTFGDGTGDHPRASAAWLTGVHAYDRTRPGVEVKLATTADQLAAQVIGK
                     DSPVASIELAIDAPLQGSCDSGDCFYVNTVSWRNDTTPNLAELHPRIVFERLFGDGGS
                     SAERLARSRSRGSILDSVSAEAARLAASLGGGDGAKLDEYLDSVREIERRIQSAESRG
                     DSDVVLPERPTDIPETYEEHARLMFDLQVLAFQTDTTRITSMIMSRELSGLSYANIAV
                     PEAHHAISHHRGDPELVAKKARIDTYHVQLLAEFAAKLHATQDGDGSLLDHSLVLYGS
                     GMGDGNLHRHADLPCLMLGKLGGALKTGQHVAYPDDTPMTNLLVTLLDKVGVHVDRLG
                     DSTGPLDPDPFST"
BASE COUNT         1019 a         1971 c         1998 g         1098 t
ORIGIN      
        1 caggccgcgg tcgactcgat cttcttggcc aacccgacgc agtacgccgg ctacttcccc
       61 tgcaacggct ggaacaccga gaccgacggc gtggcaggca tcacgttcgc cgattgcgcc
      121 cgcggagcgg ccaatgccgg cggcggccgt tgggccgtca accggacctc cgagatctac
      181 ttcaaccgtg acggctcgcc gttcgtattg gcgggcgcgc acggctacaa cagcggcttc
      241 ggcagcacgg ccatcggcga cggctacgcc ggcatgcgcc tgcaaccgaa cggcgacttg
      301 ggtcaagtcg cgttcgacgg ccaggcggct acgccgctgg agcgccgctc actgttcggc
      361 cgcgcgacgc acgagatcaa cgaccacctg accgcgttcg cgcaggcgac ttacagcggc
      421 gtcaacgtct cgacgacggg cggctacccg ccggcgatca cggtctggtc ggcgccggtg
      481 cctctcgacg ggcggccgat tccggccgca ttgcagacgc tgctcgcgtc gcgcgtgcgt
      541 aacgtcgatc ccgatggcgc cggcccggcc gttgccgtca accagggccc gaccgatccg
      601 tggcaattgt tccgcgtgct cgacttcttg ggcggcgccg tgacgacgga cgtcgatcgc
      661 gacgtctacc agatcatggc gggcgtccaa ggcagcttca gccaacgcga ctggacctgg
      721 gaggcctatg tctcctcggg tcagacgagc gaaagcgact tcttctcgaa catgccgtcg
      781 ttgcagcgtt attggaacct catcggcgcc gcgccgaacg ccctgacgtt gctcggtgcg
      841 ccgggcaacg gcacgtgggg ccgaggcacg ttcactcaag ggcgcaacta cgctcagacc
      901 tgcacgagcg gtttgccgat cttcgcgaac agctcgaact tgggccctgg ccaagtgtcg
      961 caagactgcc tcgagagcat cgccgcgaac acgcgttcga tcacggagct gagccaagag
     1021 atcgccgagt tcaacctgca gggcaagatc gttgacatga aggccggcga gctgcgcttt
     1081 gccgtcggcg tgagctcgcg caagaacgag ttttcgttcg agcccggcga gacgaacgat
     1141 cgcgagtcgg tggccgagca gccgatgagc atcttcgcgt cgaacgacac ggcaggtggc
     1201 acgaaggtgc aagagctcta cggcgagttg ctcgtccccg tgacgaacaa gttcgacctc
     1261 gagttcggcg tgcgtgcctc ggactatgtc gattcgaccg tcggtacgac gcagacgtcg
     1321 aaggccatgt tcacgttccg cgcgaccgac gccttgaccc tgcgcggcgg ctatcagaag
     1381 gccgagcgcg cggcgaacac ggcggagctg ttccaaggcg tgtcgttgct cgtcgtgccg
     1441 ttcgggccga gcgatccgtg ctcgttcacg accacggctc cgtggggcaa cgtcgcatcc
     1501 aacccgaacc gcttggcagt gcagagcctg tgcgcggcga tcatcaacaa cagcgacggc
     1561 atcacggcca acgacaacac gtcggcgttc ggcttacccg gcagcggcac ggccaacaac
     1621 ttcgcgcggc ccggcaaccc gttcttcccg ctcgagatcg agctgcgcaa gggcaacccc
     1681 gccgtgcagc ccgagaaggg cgagacgttc accttgggcc tcgtcgtcca gggcccgggc
     1741 agcctcgaga acctgacggc gtcgttcgac tggtacgaca tcgagatcac cgacgcgatc
     1801 gcgccgctga actcgctgtt cgcgcatcag cagtgcttca acgccaacgg ctcgagcaac
     1861 ccgaccttgt cttatacggg caatccgtac tgcgccttga tcttgcgcaa cgtcgtgtcg
     1921 ggtgagcgct cgtcggtcga tgcgccgttc atcaataccg gctcgttgaa gacctcgggt
     1981 atggacatca ccgtcaactg gacgacggac gtcggcgacg gcggctcgtt cttcatcaac
     2041 tcgctgctga cggtgctcga cgagtacatc gtgcaggact cgccgacggc gatcccgttc
     2101 gatgccaagg gcacgctcga ccagggcggt cagtacgact ggaagatgac gaacacgttc
     2161 gggtacaact tcagcggcgg caaagccaac gtcggtctcc aatggcgtta tttgccggag
     2221 atcaaggacg agactttcgc gcgcacaccg aacacgcgcg tgttcccggt cgactcgtac
     2281 cagacgctga acttgttcgc gggttactcg ctcaacgaga aaatcaacct gcgcatgggt
     2341 atcgacaatc tgctcgacga agacccgttg atcgttggcg cccagccggg cgacaacaac
     2401 gcggaggtca cgcgcgcgga ttacttcgac atcctcggac gtcgtgcgta cgtcggcgtg
     2461 aagatgagct tctaagacgt ttagatcgcg ttggacggac ggcgggcagc gatgcccgcc
     2521 gtcttttttt tgcggcgagc tattgctgcg gcgccggcgg cgttgccgct tccggggccg
     2581 cctcggcacg cggcggcagc tcgacctgct cggtcgtcac cgcttcctgg ctgctgcgcc
     2641 tgcgcgggcg gcggcgatcg gagtcttcgt tgttgaccgc cgctagcgtt tcgagctcgt
     2701 cttccgtgat cggcacgaat gcaccgagtt ggcaggtgaa cgccggcgtg tagttgccga
     2761 gccccgtctg ccagaggact tggaagatac tcccggcgca cagctcgcgg ttcgcgacgg
     2821 cgtagttgac caagctgttg cggttcaagc ttgggcattg gcgcggcagc tgattgttgt
     2881 agatctcgtt ccgtttcgtg acgaaggcga tgttgcgatc gcccaagatc cgcgtgcgcc
     2941 tgatcgtgtt gtgatcgagg cacgccttgg ccacggtcgc ctcgtcgtcg tcacgatcct
     3001 tgccgtcctg ggcgtttgcg ccgccggtaa tcgccgacgc gagcaacagg gcgcaagccg
     3061 ctgtgcgcgc gttgaatttg cgactgttca tcggagctcc ttctgcggac accgctcgtg
     3121 acgtgtagtc agcgtcagcg ctcgtttggt tcatccgcgc tcttgccatt tgctcctcgt
     3181 cgggcgagtc tcgtcgcttc gttttgagga gtctgtcgat gagcctacca ccgggagtca
     3241 gcgagcggga tttcgagcgg gccgtggccc gtttccgatc cgtggtcggc gcggactggg
     3301 tgttcacgag cgacgaggac acggcgctct accgcgacgc ctattcgccg ttcttcaacg
     3361 agcccgagga gaaggtggcc tcggcggccg tcgcgcccga gacggccgag cacgtcgttg
     3421 agatcgtccg aatcgccaat gagctccgaa ttcctctgta tccaatctcg acgggccgca
     3481 acttgggcta cggcgggtcg gcgcccgtgc tctcgggcag cgtcgtgctc gacttgaagc
     3541 gcatgaatcg gatcctcgag gtcgacgagc gcaacgcgta cgcgctcgtc gagcccggcg
     3601 tcagctactt cgacctctat cggcacatcg aggagcgcgg gctgaagctg tggatcgatt
     3661 gccccgaccc cggttggggc agcgtcatcg gcaacgcgct cgaccacggc gtcggctaca
     3721 cgatgaaccc gtaccgcgat cactggggcg cgcagtgcgg cctcgaggtc gtcacgggcg
     3781 acggcgagct cgtgcgcacc ggcatgggtg ccctgccgaa gtcgcgcacg tggcaacagt
     3841 tcaagtacgg cttcggcccg tatctcgacg gcatgttttc gcagtcgaac ttcggcgtcg
     3901 tgacgaagat gggcgtctgg ctcatgccgc agcccgaggc ctacgtcgac tgcaatatcg
     3961 acgttttcgg ccacgacgac atcatgccgc tcgtcgatca catgaattac gtcatgaaca
     4021 ccggcttgca gaacgcaggc accaacctgc agtcgccgat ccggttcgcg gtcgcgggca
     4081 atccggagct gcgcgcagcg ctcgcgagcg aggacccggc ggagatcgcc aagctcaacg
     4141 cgttcgcgcg cgagcgcgag gtgccgttct actcgaacgt cttcaagttc tacgggcccg
     4201 aagaggtcgt gcgcgcgcag ctcgagtaca cgcgacggcg gttactgacg attcccggcg
     4261 ctcggttccg cgaaggggcg ttctacaagt tcccgatcac ggcggagcag aaagccaacg
     4321 tgcgcgatcc gcaggatctc ggcatcccga cgctcgagac gttctcgatc ggcgcgcgct
     4381 cgaatttcgc gcccgcaacg cgcggccatt tgggcttctc gcccgtgatt ccgatgcgcg
     4441 gcgaggacgt catcgagtcg cagcgcgtgt tccagcgcgt gctgcaggag ttcggcaccg
     4501 acgtcgcatt gctcgtgttc ccgcagtcgt atcatccgcg gacgttcgtg atcctctgca
     4561 tgttctatgt catgccggac cccgcccaca acgacgccgt gcggcgcacg ttcaagcggc
     4621 tcgtcgaggt cgcggccgag cacggctggg gcgagtaccg cacgcacacg gccttcatgg
     4681 acgattgcgc cgcggcgtac tcgttcaacg accacgcgct caagaagctg caagagaaga
     4741 tcaaggacgc cgtcgatccg aacggcgtgc tctccgcggg gcgctacggc atctggccga
     4801 agcacctgcg taagtcatag aaaaacagtg gcttgcgagc gattgttgag cttgaagctg
     4861 atccagcttt aagtcgaaaa cgggtcggga tcgagcggcc ccgtgctgtc gccgagccgg
     4921 tcgacgtgca caccgacttt gtcgagcagc gtgacgagca ggttcgtcat cggcgtgtcg
     4981 tcgggatagg cgacgtgctg gccggtcttc agcgcaccgc cgagcttgcc cagcatgagg
     5041 cacggcaaat cggcatggcg gtgcaggttg ccgtcaccca tgccgctgcc gtacagcacg
     5101 agactgtgat cgagcaggct gccgtcgccg tcttgcgtcg cgtgcagctt cgccgcgaac
     5161 tcggccaaga gctgcacgtg atacgtgtcg atgcgggcct ttttggcgac gagctccgga
     5221 tcgccgcgat ggtgcgagat cgcatggtgc gcttccggta cggcgatgtt cgcgtagctc
     5281 aggcccgaga gctcgcgcga catgatcatg ctcgtgatgc gcgtcgtatc cgtctggaat
     5341 gcgagcacct gcagatcgaa catcagccgc gcgtgctctt cgtaggtctc gggaatgtcg
     5401 gtaggccgct cgggcaagac gacgtcgctg tcgccgcgcg attcggcgct ctggatgcgc
     5461 cgctcgatct cgcgcacgga gtcgaggtac tcgtcgagct tcgcgccgtc gccgccaccc
     5521 aaactggccg cgagccgcgc ggcttcggcg ctgacggagt ccaagatgct gccgcgcgag
     5581 cgtgagcgcg ccaggcgctc ggcgctcgag ccgccgtcgc cgaacaagcg ctcgaagacg
     5641 atgcgcgggt gcagctcggc gagattcggc gtcgtgtcgt tgcgccacga gaccgtgttc
     5701 acatagaagc agtcgccgga atcgcacgag ccttgcagcg gcgcgtcgat cgcgagctcg
     5761 atcgaggcga ccggcgagtc tttgccgatg acctgtgcgg ccaattggtc ggctgtggtc
     5821 gcgagcttga cctcgacgcc gggccgcgtg cggtcgtagg cgtgcacgcc cgtcagccac
     5881 gccgccgacg cgcgcggatg atcgccggtg ccgtcgccga acgtgtccgc ctgcaggtgc
     5941 gacagcccgc tcagcacgtt gatgtcggct ttgtgcgccg ccagcggctt cagaatcggc
     6001 gtgagctcgt aaccgccgcc ggtcgttgcc ggcgtccact ggtcctgaat gacgccgttc
     6061 gcgatgtaga gaaacgcgaa gcgcgg
//
LOCUS       DATLEE010000644         4925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10855640, whole genome shotgun sequence.
ACCESSION   DATLEE010000644 DATLEE010000000
VERSION     DATLEE010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10855640"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VM692_14110"
     CDS             complement(<1..1168)
                     /locus_tag="VM692_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HUQ53357.1"
                     /translation="MTAGFLVVALFVLLFLRVPIAIALGLSSILTMLLFADQSLVALA
                     QRFFSALQVYPLLAIPFFILAGALMTTGGVAKRMIAFANALVGHFRGGLAMAALLACA
                     LFAAVSGSSPATVVAVGSIMIAGMARSGYSREFAAGLICNAGTLGILIPPSIVMVVYG
                     AVTETSIGDLFIAGVVPGIMLTAMLMVAVAIRARQLDLPRQPRADLRTVLKSGRDAIW
                     GLLLVVIILGGIYRGVFTPTEAAAVSAVYAFAIAVFVYRDVRLREVPHVVADAAKVTA
                     MLMFIIGNAYLFAFVLTTEQIPQMMSAWITDLGLPPWAFLVMLNLLLLVAGSFMEATS
                     VVLIFAPIMFPIATSLGIHPIHLGIIMTVNMEIGMITPPVGLNLFVTSGISQLPV"
     gene            complement(1165..1668)
                     /locus_tag="VM692_14115"
     CDS             complement(1165..1668)
                     /locus_tag="VM692_14115"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HUQ53358.1"
                     /translation="MATRTQRALKAADRLEEAFMIAALSFMTLLTFLQVVLRYGFGTG
                     FVWSLEATTYSFAWLVLIGMSYGVRTGAHIAVDLLTSRLTPSGARGVAAVALLFALAY
                     CALMIYGSGQFVDRLMALGTNARDIPLPRWVLTGIMPIAFLLLALRFVQAARPVLWPR
                     AAAGDAQ"
     gene            complement(1681..2703)
                     /locus_tag="VM692_14120"
     CDS             complement(1681..2703)
                     /locus_tag="VM692_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HUQ53359.1"
                     /translation="MTVQARVRAVLLGFSLAALSACGGSDGAGATASATQLYVIKFPH
                     VVATATPKGQMAQRFKELAEERFPGRVRVEVYPSAQLMEDTESIEALAFGEVQMIATS
                     VSLYDRLTQKFQVFDLPFLFPNLAAVERFQASAAGRELLTTLVDQGILGLQFWHNGMK
                     QFTGPRPLIEPDDARGLKFRVMESDILQAEIEAIGGSPQKMAFGEVYQALQTGTVDAQ
                     ENTWSNIYSSKFYEIQPYMTESNHGYIGYFVAVNAKFWQGLPDDLRTGLESTLAEVTQ
                     WGNARSEAINQEDKQRIIDSKRAEITVLTPLQLEAWQAAMRPVWDEFRDRIGGELVDA
                     ALAAAQ"
     gene            2920..3933
                     /locus_tag="VM692_14125"
     CDS             2920..3933
                     /locus_tag="VM692_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HUQ53360.1"
                     /translation="MAGFTARNPQLSISTETRLKLYRTMLECRVLEKRAYDLFMQNLI
                     KGTSHLALGQEATATGFGLAMRPDDYTFCTYRGHNHTLVRGVGMTEILAELMGNSAGL
                     MRGKGGSMHLTSVKHNMMGSYAIIGAQLTIANGAAWSAQYRGTEQVAVCFFGDGTTNI
                     GAFHEALNFAVIWKLPVVFVCENNLYMEYTSIHDVTAVEHPAADRASAYGLEPILIDG
                     NDVDVVYRTAQRALELARKGGGPSLIEAETYRHGGHSRADPGKYRPDEEVKAWLAKDP
                     LPRYRARLLEMGINVADIEKIETETNAEIDRATEIARNAPPPPLEIADKDVWADGGSS
                     WRN"
     gene            3921..4898
                     /locus_tag="VM692_14130"
     CDS             3921..4898
                     /locus_tag="VM692_14130"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HUQ53361.1"
                     /translation="MAQLTYRDAVAAGIAQEMRRDPNVVLLGEDVAAAGGVFKATVGL
                     LDEFGAKRVKDTPISEQAIVGAAMGAAMTGLRPVVDLMFSDFFAVAWDIVANQIAKTR
                     YMTDGQVKLPLVIRSANGAGSRFGAQHSQSVENWSMMIPGLKIVAPAFPGDVKGLLAA
                     AIRSDDPVMFFEQKSLYATKGEVPDGEHFIDLGKANVLRDGKDVTIVALALMVPRALK
                     AADMLKEQHGIDATVVDLRSLVPLDTQTILREVGKTGRLVTVEENPRLCGWGAEIASI
                     VAEELFWDLDGPIVRVTTPHVPLAAANNLEDAQLPTPERLVESVRKLVD"
BASE COUNT          977 a         1641 c         1560 g          747 t
ORIGIN      
        1 tcacgggcag ctgcgaaatg ccggacgtca cgaacaggtt gagcccgacc ggcggcgtga
       61 tcatgccgat ctccatgttg accgtcatga tgatgcccaa gtggattgga tgaatgccga
      121 gcgaagtggc gatcgggaac atgatcggcg caaagatcag gaccaccgac gtcgcttcca
      181 tgaagctgcc cgcgacgagc agcagcagat tcaacatgac gagaaacgcc cacggcggca
      241 ggcccaggtc cgtgatccac gccgacatca tttgcgggat ctgctcggtc gtcagcacga
      301 acgcgaacag atacgcgttg ccgatgatga acatcagcat cgccgtcacc ttcgcggcgt
      361 cggcgaccac gtgcggcacc tcgcgcaagc gcacgtcgcg gtagacgaac acggcgatcg
      421 cgaacgcata gacggcggac acggccgcgg cttccgtcgg cgtgaagacg cctcgataga
      481 tgccgcccaa gatgatgacg accagcagta ggccccagat cgcgtcacgc ccgctcttca
      541 gcaccgtgcg caaatcggcg cgcggctgac gcggcaagtc gagctgtcgc gcgcgtatcg
      601 cgacggcgac catcaacatc gccgtcagca tgatgccggg cacgacgccg gcgatgaaca
      661 agtcgccgat cgacgtttcg gtcaccgcgc cgtagacgac catgacgatc gacggcggaa
      721 tcaaaatgcc gagcgtgccc gcgttgcaga tcaacccggc cgcgaactcg cgcgagtagc
      781 ccgaacgcgc catgccggcg atcatgatcg aaccgacagc gacgacggtg gccggcgacg
      841 acccggacac cgcggcgaac agcgcgcagg caagcagcgc ggccatcgcg aggccgccgc
      901 ggaagtggcc gacgagcgcg ttcgcgaacg cgatcatgcg cttcgcgact ccgcccgtcg
      961 tcatcagcgc gccggccaag atgaagaacg gaatcgcgag cagcgggtaa acctgcagag
     1021 cgctgaagaa gcgctgcgcc aacgcgacga gcgactggtc ggcgaacagc agcatcgtca
     1081 ggatgctcga aagcccaagc gcgatcgcga tcggcacgcg caggaacagc agcacgaaca
     1141 gcgcgacgac gaggaagccg gccgtcactg tgcgtcgccc gccgccgccc gcggccacag
     1201 aaccggacgc gcggcctgca cgaagcgcag tgcgagcaac aagaacgcga tcggcatgat
     1261 gcccgtgagc acccagcgcg gcagggggat gtcgcgtgcg ttcgtgccga gcgccatcag
     1321 cctgtcgacg aactgaccgc tgccgtagat catcagggcg cagtacgcga gggcgaataa
     1381 caacgcgacc gcggcgacgc cgcgcgcgcc gctcggcgtc agtcggctcg tcaggaggtc
     1441 gacggcgatg tgcgccccgg tgcgcacacc gtacgacatg ccgatcaaca cgagccacgc
     1501 gaacgagtac gtcgtggcct cgagcgacca gacgaacccc gtcccgaacc catagcgcag
     1561 cacgacttgc aggaaggtca gcagcgtcat gaacgagagc gccgcgatca tgaacgcctc
     1621 ttcgaggcgg tcagccgcct tcagcgcgcg ctgcgtccgc gtcgccactg cgcgctcgag
     1681 ctactgagcc gctgccaacg ccgcgtcgac gagctcgccg ccgatgcggt cgcgaaactc
     1741 gtcccagacc ggccgcatgg cggcctgcca agcttcgagc tgaagcggcg tcagcaccgt
     1801 gatctcggcg cgtttcgaat cgatgatgcg ctgcttgtct tcttgattga tggcttcgga
     1861 gcgcgcatta ccccattgcg taacttccgc gagcgtcgac tcgagccccg tgcgcaggtc
     1921 gtccggcagc ccttgccaga acttggcgtt caccgcgacg aaataaccga tgtagccgtg
     1981 attgctctcg gtcatgtacg gctggatctc gtagaacttc gacgagtaga tgttcgacca
     2041 cgtgttctct tgcgcgtcga cggtgcccgt ttgcagcgcc tgatagacct cgccgaacgc
     2101 catcttctgc gggctgccgc cgatggcttc gatctccgct tgcaggatgt cggattccat
     2161 gacgcggaat ttcaagccgc gcgcgtcgtc gggctcgatc agggggcgcg gccccgtgaa
     2221 ctgcttcatg ccgttgtgcc agaactgcag cccgagaatt ccctggtcga ccagcgtcgt
     2281 cagcagctcg cgccccgccg cgctcgcctg aaaccgttcg acggcggcga ggttcgggaa
     2341 caggaacggc aagtcgaaca cctgaaactt ctgcgtgagg cggtcgtaca gcgagaccga
     2401 cgtcgcgatc atctgcacct cgccgaacgc aagcgcttcg atcgactccg tgtcctccat
     2461 gagctgcgcc gacggataca cttcgacgcg cacgcgccca ggaaagcgct cttcggcgag
     2521 ctccttgaac cgctgcgcca tttgaccttt cggcgtggcg gtcgcgacca cgtgcggaaa
     2581 cttgatgacg taaagctgcg tggcgctcgc ggtggcaccc gcaccgtccg agccgccgca
     2641 tgccgaaagc gccgctaaag aaaacccgag caaaaccgct cgaacgcgcg cctgcaccgt
     2701 catgcgttta gccccccgta cgacctcatt cggttaagga cagtctggcc gacgtgcggc
     2761 catcttgcta ggctaggcgg cctccaagac aaggaagacc ggtcggtttg ccggcaacgt
     2821 cgccaggacg gctgcaaccc cctctcgcat agaaaaccct catagaacaa acgccccttt
     2881 cctttttgaa aaggcaactt agcagtggaa gcacagacga tggcgggttt caccgcccgt
     2941 aacccgcagc tgtcgatcag taccgaaact cgattgaagc tgtaccgcac gatgctcgag
     3001 tgtcgcgtgc tcgagaagcg cgcgtacgac ttgttcatgc agaacctcat caaaggcacg
     3061 agccatctcg cgctcggcca agaggcgacc gcgaccggct tcggtctcgc gatgcgcccc
     3121 gacgactaca cgttctgcac gtaccgtggc cacaaccaca cgctggtccg cggcgtcggc
     3181 atgacggaga tcttggccga gctcatgggc aactcggccg gcctcatgcg cggcaagggc
     3241 ggctcgatgc acttgaccag cgtcaagcac aacatgatgg gctcgtacgc gatcatcggc
     3301 gcgcagctca cgatcgcgaa cggcgcggcg tggtcggcgc agtatcgcgg cacggagcag
     3361 gtcgcggtct gcttcttcgg cgacggcacg acgaacatcg gcgcgttcca cgaggcgctg
     3421 aacttcgccg tgatctggaa gctgcccgtc gtgttcgtct gcgagaacaa cctctatatg
     3481 gagtacacgt cgatccacga cgtgacggcc gtcgagcatc cggccgccga tcgcgcgagc
     3541 gcgtacggct tggagccgat cctcatcgac ggcaacgacg tcgacgtcgt ctatcgcacg
     3601 gcgcaacgcg cgctcgagct ggcgcgcaaa ggcggcggcc cgagcttgat cgaggccgag
     3661 acgtaccgcc acggcggcca ctcgcgcgcc gaccccggca agtatcgtcc cgacgaggaa
     3721 gttaaagcgt ggctcgccaa agacccgctg ccgcgttacc gcgcacgctt gctcgagatg
     3781 ggaatcaacg tcgccgacat cgagaagatc gagacggaga cgaacgcaga aatcgatcgc
     3841 gccacggaga tcgcacgcaa cgcaccgccg ccgccgctcg aaatcgccga caaggacgtg
     3901 tgggctgacg gaggctcctc atggcgcaac taacgtatcg tgacgcggtc gccgcgggca
     3961 tcgcacaaga gatgcgccgc gacccgaacg tcgtgctgct tggcgaggac gtcgccgcag
     4021 ccggcggcgt gttcaaggcg accgttggcc tgctcgacga gttcggcgcg aaacgcgtca
     4081 aggacacgcc gatctcggag caagcgatcg tcggcgccgc gatgggcgcc gcaatgacgg
     4141 gcctgcgtcc cgtcgtcgac ttgatgttct cggacttctt cgccgtcgcg tgggacatcg
     4201 ttgcgaatca gatcgcgaag acgcgctaca tgacggacgg ccaggtcaag ctgccgctcg
     4261 tgatccgctc ggccaacggc gccggctcgc gcttcggcgc gcagcattcg cagagcgtcg
     4321 agaactggtc gatgatgatt ccgggcctca agatcgtcgc gccggccttc cccggcgacg
     4381 tcaaaggcct gctcgccgcg gccatccgca gcgacgaccc cgtgatgttc ttcgagcaga
     4441 agtcgctgta cgccacgaag ggcgaagtcc ccgacggcga gcacttcatc gatctcggca
     4501 aagctaacgt ccttcgcgac ggcaaggacg tcacgatcgt cgcgttggcg ttgatggtgc
     4561 cgcgcgcgct aaaagctgcg gatatgttga aagagcagca cggcatcgac gcgaccgtcg
     4621 tcgatctgcg ctcgctcgtg ccactcgaca cgcaaacgat cctgcgcgag gtcgggaaga
     4681 cgggccggct cgtcaccgtc gaagaaaacc cgcggctctg cggttggggc gccgagattg
     4741 cctcgatcgt cgccgaggag ctcttctggg acctcgacgg cccgatcgtg cgcgtcacga
     4801 cgccgcacgt tccgctcgcg gccgcgaaca acctcgagga cgcgcaactg ccgacgcccg
     4861 agcgcctcgt cgaaagcgtg cgcaagctcg tcgactaaaa taaagccacg gagagggctc
     4921 gatga
//
LOCUS       DATLEE010000645         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3424651, whole genome shotgun sequence.
ACCESSION   DATLEE010000645 DATLEE010000000
VERSION     DATLEE010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3424651"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VM692_14135"
     CDS             complement(<1..420)
                     /locus_tag="VM692_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HUQ53362.1"
                     /translation="MSNELIVRRDGAVLTVTFNRPQRHNAMTYEMYDGLFAACEAADG
                     DGGVKALVLRGAGGKAFVSGTDIASFRSFSSGGDGVEYEQRIARVVNRLEDVRVPTVA
                     AVEGYCVGGGLAIAAVCDLRIATRSSRFGMPIARTLGN"
     gene            complement(417..1610)
                     /locus_tag="VM692_14140"
     CDS             complement(417..1610)
                     /locus_tag="VM692_14140"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HUQ53363.1"
                     /translation="MSNALDGIRVLEIGVFIAAPFAALQLADLGADVTKVESLDGEPV
                     RASGPFVSGESSPFLRLNRHKRSVALDLKSPGGKRAFLRLAATADVIVENLRPGALRA
                     LQLGYDDIRAVNPRVVYASASGFGQDGPLADRPGLDIMAQARGGLMSITGEAGGAPAK
                     IGVPICDLVAGLYAALGIVAALRERDTSGVGQYLDVSLLESGVSLAVWEAGRFFATGE
                     VGRPLGTAHQSEAPYQAVRSADGWLTLGAITPKTWTALCKALALERLEHDARFATSSL
                     RHAHRHELIPSIEERTMTMSTAANVQRLEALGVPCAPIADYGQVFTDEHLAARNFFWD
                     APHPVAGPVRQLGSPLRLSRSTPRRGAAAPTLGADTRTVLREVGYSDAEIDALVTAGS
                     VGVGK"
     gene            1827..>2182
                     /locus_tag="VM692_14145"
     CDS             1827..>2182
                     /locus_tag="VM692_14145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUQ53364.1"
                     /translation="MKILLVEDDADDADFLRVSLSQRDYSVSVTRTSLLKDAVNALDT
                     DQFDVVLLDLNLPDGRGAECVQKIQETDGLVPIVVLSGHGDEDYAVEILNRGVQDYLV
                     KWEGDGRTILRAIRYA"
BASE COUNT          361 a          746 c          735 g          340 t
ORIGIN      
        1 gttgccgagc gtgcgcgcga tcggcatgcc gaagcggctc gagcgagtcg cgatgcgcaa
       61 atcgcagacc gcggcgatgg ccagcccgcc gccgacgcag tagccctcga cggccgcgac
      121 ggtcggaacg cgaacgtcct cgagccggtt cacgacgcgc gcgatgcgct gctcgtattc
      181 gacgccgtcg ccgccggagc tgaagctgcg aaagctcgcg atgtcggtgc ccgacacgaa
      241 tgctttgccg ccggcgccgc gcagcacgag cgctttgacg ccgccgtcgc cgtcagccgc
      301 ctcgcaggcg gcaaagaggc cgtcgtacat ctcgtacgtc atcgcgttgt gccgctgcgg
      361 ccggttgaac gtcaccgtga gcacggctcc gtcgcggcgc acgatgagct cgttgctcac
      421 ttgccgactc ctaccgagcc ggcggtgacg agggcgtcga tctcggcatc ggaatacccg
      481 acctcacgca gcacggtgcg cgtgtctgcg cccaacgtcg gcgccgccgc gccgcgccgc
      541 ggcgtgctgc gcgaaagacg caacggcgag ccgagctgac gcacgggtcc ggccacggga
      601 tgcggcgcat cccagaagaa gttgcgcgcg gccaggtgct cgtcggtaaa gacctggcca
      661 tagtcggcaa tcggcgcgca cggcacgccg agggcctcga ggcgctgaac attggcggcc
      721 gtggacatcg tcatcgttcg ctcctcgatc gacggaatca gctcgtgccg gtgcgcgtgg
      781 cgcagcgatg aagtcgcgaa ccgggcgtcg tgctcgagac gctcgagcgc gagcgccttg
      841 cacagcgcgg tccaagtctt cggcgtgatg gcgccgagcg tcaaccaccc gtcggcgctg
      901 cgcacggctt ggtaaggcgc ctcgctctgg tgcgcagtgc cgagcggccg cccgacttcg
      961 cccgtcgcaa aaaatcgccc ggcttcccaa accgccagcg aaactccgga ctcgagcagc
     1021 gacacgtcga ggtactgccc gacgccactc gtgtctcgct cgcgcagcgc cgccacgatg
     1081 ccgagggcgg cgtagagacc ggcgacgagg tcgcagatcg gcacgccgat tttcgcgggc
     1141 gcgccgcctg cttcgcccgt gatgctcatc aatccgccgc gcgcttgcgc catgatgtcg
     1201 aggcccggcc ggtcggcgag cggcccgtct tgaccgaacc ccgacgcgga cgcgtagacg
     1261 acgcgcgggt tcacggcgcg gatgtcgtcg tagccgagct gcagagcgcg cagcgcgccg
     1321 ggccgcaaat tttcgacgat cacgtccgcg gttgccgcga ggcgcaggaa cgcgcgcttg
     1381 ccaccgggcg acttgaggtc gagcgcgacg gaccgcttgt ggcggttcag ccgcagaaac
     1441 ggcgagctct cgccgctgac gaacgggccc gacgcacgca cgggctcgcc atcgaggctc
     1501 tcgaccttcg tgacgtcggc gccgagatcg gcgagctgca acgccgcgaa cggcgccgcg
     1561 atgaacacgc cgatctcgag cacgcggata ccgtcgagtg cgttactcat atcgctgcgg
     1621 catggtaccc ggttttcgag gctgcgcccc cgccacgccg cggctcagcg gcattcttgc
     1681 gaacacgtag cccgcaaaaa ctgccacgcc ccagttgctc taaggttcag accggttttt
     1741 tgtgattggc gccctgaccg cgtgcgtttc tcttgctagt ttccgaaccg cagatccggg
     1801 gggaccatgc agcgagcggt tgcgccatga agatactgtt agtcgaggac gacgccgacg
     1861 acgccgattt tctgcgcgta tcgctgtcac agcgcgacta ctcggtctcc gtaacgcgca
     1921 cgagcttgct caaagacgct gtgaacgcgc tggacaccga tcagttcgac gtcgtgctgc
     1981 tcgatctgaa tctgcccgac gggcgcggcg ccgaatgcgt gcagaagatt caggaaaccg
     2041 acggtctcgt gccgatcgtc gtgctgagcg gccatggcga cgaggattac gccgtcgaga
     2101 tcttgaaccg cggcgtgcag gactacctcg tcaagtggga aggcgacggg cgcacgatat
     2161 tgcgcgcgat ccgctacgcc at
//
LOCUS       DATLEE010000646         3509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10089997, whole genome shotgun sequence.
ACCESSION   DATLEE010000646 DATLEE010000000
VERSION     DATLEE010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10089997"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="VM692_14150"
     CDS             <1..1690
                     /locus_tag="VM692_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ53365.1"
                     /translation="DHSWEEMTSAFGTTDRVLILKKPFDTVEVRQLAHALQRRWELAR
                     LASIKVHDLTALIDAQTAELRSANEALKQEAALREEALRRLGESNEQIRALAYQDGLT
                     GLPNRRLFNEHLEKVLARSRRKGTEFAVLFIDIDNFKLINDTIGHQAADGVLRQLADS
                     LALLIRTDDLMGLYREQDTDTTATITVEPIHDSVLSRLGGDEFIVLLPDTRDRFTAGA
                     VARRILEHLEQPIRVEGHEVFVSASIGIATYPEDGLSSEILVRNADTAMYHAKEQGKA
                     AFQYYSAAMNAASVERLTLETGLRRALEDGSLELHYQPQVDVRSGRILGAEALLRWHH
                     PQRGYISPTSFIPIAEDTGLILPLGQWVFDRACAQAAEWQRAGFPKIPIAVNVSGVQF
                     QRQDLANVVRRTLASYRLDPSLLHIEITETAIVSARERAVALLEQLRTLGVGLALDDF
                     GTGYSSLSYLKSFPIDTLKIDRSFVAEMLTDHTTASIIEAIISMTRILGLSVIAEGVE
                     DYSQFAFLKRIGCDAVQGFYVSGAVPAADFEKLLKSFDGVSFLLGNGRRGASAG"
     gene            complement(1687..3450)
                     /locus_tag="VM692_14155"
     CDS             complement(1687..3450)
                     /locus_tag="VM692_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HUQ53366.1"
                     /translation="MTVRNQWSPGPAAWQDDLAPLPAAEWSYDRAAHLLAHAGFGGSP
                     QEIQALAELGVERAVESLVHYERTANPLLAPFVESGLWDPSLATFPDSRPEATDRAEQ
                     HGESMGVSVKPAGNRALQPVSDRFFYWLRATMLETRRLGYWWANRMLATTHPLEEKMA
                     LLWHGHFATHENKVRDYRKMHQQIDLFERYATGNLRELTIAVARNPAMLYFLDAQYNV
                     KGAPNENFAREVMELFTMGVGNYTERDVRECARAFTGWYFDDLAFKVDTAKHDSATKT
                     FLGRSGDFDGVDVLGIIFEQPVTAEYLAAKIYRFLVRDELSSGLRTKLGAVLRDADYE
                     VKPLLTAILRSKDFYSQASYGSHIKGPVEHIVAMMKHLGVRTMPGVPDFNQSTIAMGQ
                     HLLNPPSVAGWAGGKAWITPGLLIARGNVARDVLIPDMTGFSDWNFNAGSDDVLGQRL
                     RDGFDVGAATAVSDPANMSTFDRVALERDEKFNTRISGYIGWEQAARKLIPTPRDAAR
                     FDLGEMVAAAGATTAAAAVDYLLGRLLRVPAANETRDALVAFLTSELGTEDLERAKTY
                     SEDALRMTAHLVMSMPEYQLV"
BASE COUNT          584 a         1151 c         1154 g          620 t
ORIGIN      
        1 ggatcacagc tgggaggaga tgacgtccgc gttcggtacg acagaccgtg tgctgatctt
       61 gaagaagccg ttcgataccg tcgaagtgcg gcagctcgcg cacgcgctgc aacggcggtg
      121 ggagctcgcg cgcttggcgt cgatcaaggt gcacgacctg acggcgttga tcgacgcgca
      181 gacggccgag ctccgatccg cgaacgaagc gctgaagcag gaagccgcat tgcgcgagga
      241 agcgctgcgg cggctcggcg aatcgaacga gcaaatccgg gcgctcgcgt atcaagacgg
      301 cctgacgggc ttaccgaatc ggcggctgtt caacgagcac ctcgagaaag tgctggcacg
      361 ctcgcgccgc aagggcaccg agttcgcggt cctgttcatc gacatcgaca atttcaagct
      421 catcaacgac acgatcggcc atcaggccgc ggacggcgtc ttgcggcagc tcgcggactc
      481 gctcgccctc ttgatccgca ccgacgatct gatggggctg tatcgcgagc aggacaccga
      541 cacgacggcg acgatcaccg tcgagccgat tcacgactcc gtgctctcgc ggctcggcgg
      601 cgacgagttc atcgtgttgc tgcccgacac gcgcgaccgc ttcacggcgg gagccgtggc
      661 gcggcggatc ctcgagcatc tcgagcagcc gatccgcgtg gaaggccatg aggtcttcgt
      721 ctcggcgagc atcggtatcg cgacttatcc cgaggatggg ctgtcgagcg agatcttggt
      781 tcgcaacgcc gatacggcga tgtatcacgc gaaggagcag ggcaaggcgg cgttccagta
      841 ttactcggcg gccatgaatg cggcctccgt cgaacgcttg acgctcgaga ccggtttgcg
      901 ccgtgcgctc gaggacggca gcctcgagct ccattatcag ccgcaagtcg acgtccgatc
      961 ggggcgcatc ctcggcgccg aggcgctgct gcgttggcac cacccgcagc gcggctatat
     1021 ttcgccgacg tcgttcattc cgatcgccga ggacacgggt ctcattttgc cgctcggcca
     1081 atgggtgttc gaccgcgcct gcgcgcaagc cgccgaatgg cagcgcgcgg ggttcccgaa
     1141 gattccaatt gccgtcaacg tttcgggcgt gcagttccag cgtcaggacc tcgccaacgt
     1201 cgtccgccgc acgctcgcga gctatcggct cgacccgtct ctgttgcaca tcgagatcac
     1261 ggaaacggcg atcgtctcgg cgcgcgaacg cgcggtcgcg ttgctcgagc agctgcggac
     1321 tctcggcgtg ggtttggcgc tcgacgactt cggcacgggc tactcgtcgt tgagctatct
     1381 gaagagcttc ccgatcgata cgctgaagat cgaccggtcg ttcgtcgccg agatgctgac
     1441 cgaccacacg acggcctcga tcatcgaagc catcatctcg atgacgcgga tactcggtct
     1501 cagcgtcatc gccgaaggcg tcgaggacta ttcgcagttc gcgttcctga agcgcatcgg
     1561 ctgcgacgcg gtgcaaggct tctacgtcag cggcgcggtg ccggccgcgg acttcgagaa
     1621 gctgctcaag agcttcgacg gggtctcgtt tctgctcggc aacggccggc gcggcgcctc
     1681 ggccggttag accagctgat actccggcat gctcatgacg aggtgcgccg tcatgcgcag
     1741 cgcgtcctcc gaataggtct tcgcccgctc gagatcttcg gtgccgagct cgctcgtcaa
     1801 gaacgcgacc aacgcgtcgc gcgtctcgtt cgcagccggc acgcgcagca agcgcccgag
     1861 caagtagtcg acggcagccg cggccgtcgt cgccccggcg gcggcaacca tctcgccgag
     1921 gtcgaagcgc gcggcatcgc gcggcgtcgg aatcagcttg cgtgcagcct gctcccagcc
     1981 gatgtagccc gagatccgcg tgttgaattt ctcgtcgcgc tccagcgcga cgcgatcgaa
     2041 cgtcgacatg ttcgcgggat cgctgacggc tgtcgctgcg ccgacgtcga agccgtcgcg
     2101 caaccgttgg ccgagcacgt cgtcgctgcc cgcgttgaag ttccagtcgc tgaagcccgt
     2161 catgtcggga atcagcacgt cgcgcgcgac gttgccgcgc gcgatcaaaa gccccggcgt
     2221 gatccaagct ttgccgccgg cccagcccgc gaccgacggc ggattcaaga gatgctggcc
     2281 catcgcgatc gtcgactggt tgaagtcggg cacgccgggc atcgtgcgca cgccgaggtg
     2341 cttcatcatg gcgacgatgt gctcgacggg ccctttgatg tggctgccgt agctggcctg
     2401 gctgtagaag tccttcgagc gcaggatcgc cgtcagcagc ggcttgacct cgtagtctgc
     2461 gtcacgcagc acggctccga gcttcgtccg taatcccgaa gaaagctcgt cgcgcacgag
     2521 gaagcgatag atcttcgccg cgagatactc ggccgttacg ggctgctcga agatgatccc
     2581 gagcacgtcg acgccgtcga agtcgccgct gcgccccagg aaggttttgg tggcggagtc
     2641 gtgtttcgcc gtgtcgactt tgaaagccag gtcgtcgaaa taccaacccg tgaacgcgcg
     2701 cgcgcactcg cggacgtcgc gctccgtgta gttgccgacg cccatcgtga agagctccat
     2761 gacctcgcgc gcgaagttct cgttcggcgc tcccttgacg ttgtactgcg cgtccaaaaa
     2821 atagagcatc gccggattgc gcgcgaccgc gatcgtcagc tcccgcaagt tgccggtcgc
     2881 gtagcgctcg aacaagtcga tctgctggtg catcttgcgg tagtcgcgca ccttgttctc
     2941 gtgcgttgcg aagtggccgt gccagagcag cgccatcttc tcttcgagcg ggtgcgtcgt
     3001 cgcgagcatg cgattcgccc accagtagcc caagcgccgc gtctcgagca tcgtcgcgcg
     3061 cagccagtag aaaaagcggt ccgacacggg ctgcaacgca cggttgccgg ccggtttgac
     3121 ggagacgccc atcgactcgc cgtgctgctc ggcgcgatcc gtggcctcgg gcctgctgtc
     3181 ggggaacgtc gcgagcgacg ggtcccagag tccggactcg acgaacggcg cgagcagcgg
     3241 gttcgcggtg cgctcgtagt gcacgagcga ctcgacggcg cgttcgacgc cgagctcggc
     3301 cagcgcttgg atctcctggg gcgagccgcc gaagcccgcg tgcgcgagca ggtgcgcagc
     3361 gcgatcgtag ctccattccg ctgccggcag cggcgcgaga tcgtcttgcc aagctgcggg
     3421 ccccggggac cactggttgc gcaccgtcat cttgcgtctc cgcgtcaggc cttcagcagg
     3481 cccagggtct cgaaccgttg gccgagcac
//
LOCUS       DATLEE010000647         4558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6360112, whole genome shotgun sequence.
ACCESSION   DATLEE010000647 DATLEE010000000
VERSION     DATLEE010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6360112"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VM692_14160"
     CDS             complement(<1..385)
                     /locus_tag="VM692_14160"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HUQ53367.1"
                     /translation="MRLELRAKRRTLSDAVRRQADHAILAHIRSLPEFRRAQRVALFI
                     AFDGEPSLASLVESVRRRKRLYVPVLRGMTMTFAELEPSAPLAPNFFGILEPQLGPKL
                     DARDLDLVLTPLVGFDDHGVRIGVGR"
     gene            complement(417..601)
                     /gene="ssrS"
                     /locus_tag="VM692_14165"
     ncRNA           complement(417..601)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VM692_14165"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(612..932)
                     /locus_tag="VM692_14170"
     CDS             complement(612..932)
                     /locus_tag="VM692_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009021637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HUQ53368.1"
                     /translation="MSGAGTFAHVTIRILEKEYNVACPVEEKASLLASAELLNVKMRE
                     IRDSGKVVGLDRVAVMAALNLANELLRAKGQDEELKNLVGNRIRAMRDQLDSALGPAK
                     QLSL"
     gene            complement(929..1162)
                     /locus_tag="VM692_14175"
     CDS             complement(929..1162)
                     /locus_tag="VM692_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HUQ53369.1"
                     /translation="MTPDQANHRFDLDLRRLEKRLEELVVVCRQLQEENKSLRTRQDS
                     LMSERASLLQKNEQVRGRVEAMIGRLKGMEQVS"
     gene            1252..1839
                     /locus_tag="VM692_14180"
     CDS             1252..1839
                     /locus_tag="VM692_14180"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HUQ53370.1"
                     /translation="MTPSSALPPLDYDRLRESLANAGAVVALAELHGGVCGALCAGGA
                     PAARRWLEDCLDDQELATSTAAVAEELEELIGASEATLANPDLKFEPLLPDDDAPLDE
                     QVQALALWCQGFLSGVGTTAPRAALPSDEGSSLAEILRDFAEISRAGLSEEEAAGQAQ
                     ADFALAEIHEFVRVSVAVVFEELAALRAAATRDIH"
     gene            1872..3176
                     /locus_tag="VM692_14185"
     CDS             1872..3176
                     /locus_tag="VM692_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HUQ53371.1"
                     /translation="MNPKEFARRRRQLMELIGTSGIAILAAAPERVRSRDTLYVYRQD
                     SDFYYLTGFGEPEAVAVLVPGRPQGEFLMFCRERNPEREQWDGPRAGPEGAVAAYGAD
                     DAFPISDVDDILPGLLERSDRVYYSIGGNEFDQKLLAFIQALNSKRQSGHAPSELVAL
                     DHLLHEMRLFKSRAETGVMRQAARIAVSAHSRAMRACQPGLKEYELEAEFVHEFRRHG
                     AECSYPPIVAAGVNACILHYRSNDAPLLDGDLVLIDAGCEYGMYASDVTRTFPVNGRF
                     SPAQRELYEVVLEANQAATAKAMPGNHWNEPHDAAVGVVTRGLKDLGLLAGRVPQLVK
                     AMAYRQFFMHKTGHWLGLDVHDVGDYKVADQWRLLEPGMVMTIEPGVYVAANDKSVDK
                     RWRGIGIRIEDDVQVTREEPEVLTQGLPTTVAEIERHMGSAA"
     gene            3173..4423
                     /gene="ubiH"
                     /locus_tag="VM692_14190"
                     /gene_synonym="visB"
     CDS             3173..4423
                     /gene="ubiH"
                     /locus_tag="VM692_14190"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF004356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HUQ53372.1"
                     /translation="MNGQGRDCDVLIAGGGAVGSALACALAELPLTVVLVEAQQVRLL
                     EQPSFDARVTALANGSQQILAGLDLWPELKGYAQAIRSIHISERGRFGAARIDAREEH
                     VPALGYTIENLVLGRVLWERLQRAPRLTVLAPAKVTDVARVPEHALVSLQHGAAVETV
                     RAKLVVAADGVRSAVRAALGVETSEHDYAQRAVIFNCSTEAPLEGRAYERFTARGPIA
                     LLPLAGGRAAVIWTLPEGDAQRTAALSADDFRAELQAAFGQRLGRFTRIGERHLYSLA
                     RVASGAIHGERAVLIGDAALRLHPVAGQGFNLALRDVATLAEVIADALRAARTLGSAV
                     DVGAAELLERYAAWRASDRQRVSSFTHGLIQLFGESAPGIGLGRGLGLMAFDLLPGAK
                     ALLARQTMGKAGRLPRLARGLKLS"
BASE COUNT          718 a         1527 c         1567 g          746 t
ORIGIN      
        1 cgcggccgac gccgatcctg acgccgtgat cgtcgaaacc gacgagcggc gtcagcacga
       61 ggtcgagatc gcgggcgtcg agcttcgggc cgagctgcgg ttccaagatg ccgaagaagt
      121 tgggcgcaag cggagcgctc ggctcgagct cggcgaaggt catcgtcatg ccgcgcaaca
      181 cgggaacgta gagacgtttg cgccgacgta ccgactccac gagcgaggcg agcgacggct
      241 cgccgtcgaa cgctatgaac agcgcgactc gctgcgcgcg gcggaactcg ggcaggctgc
      301 gaatatgggc caggatggcg tggtccgctt gcctacggac cgcgtcggat agcgtgcgtc
      361 gcttggcgcg cagctcaagc cgcaactgcc gccgggccgg gtcggttgcg ggagagatgc
      421 tccccgcctg tgccgtcacg aaccgttgct ctcgaaccgg agcaacaagt ggggtacacc
      481 gtcaggactt aaggcttccc ggttggaccg gatttgcaca ccatcgccga cagggcttaa
      541 ccccggagtc atgatttagg gtccgagaga ctttcgtccg cgtcgtacac cgcagggagc
      601 accaaggaca attataggct gagctgcttc gccgggccga gtgcagaatc gagttggtcg
      661 cgcatcgcgc gaatcctatt gcccacgagg ttcttgagct cctcgtcttg acctttggct
      721 cgcagcagct cgttcgcgag attcagcgcc gccatcacgg ccacgcggtc gagcccgacg
      781 accttgccgc tgtcgcggat ctcgcgcatc ttgacgttca agagctcggc cgaggccagc
      841 aacgacgcct tctcctcgac ggggcaggcg acgttgtatt ctttctctag aatgcggatc
      901 gtgacgtgcg cgaacgtgcc ggcgccgctc acgaaacctg ctccatccct ttgagccggc
      961 cgatcatggc ctcgactcgg ccgcggactt gctcgttctt ttgcagcaag gaggcgcgct
     1021 ccgacatcag cgaatcttgc cgtgtgcgca gggatttatt ctcttcttgt agctgccggc
     1081 aaacgacgac gagctcttcg aggcgcttct cgaggcgccg caagtcgagg tcgaagcggt
     1141 gattggcttg gtcgggcgtc atgggcgcga atatagaacc gcggtgattg acggtcaaca
     1201 acacgtcggc ggtgatagca ttccaagcgc gcacactgcg cggaatcgca cgtgacaccg
     1261 agctccgcac ttccccctct cgactacgat cgcttgcgcg agtcgctcgc gaacgcgggc
     1321 gccgtcgtcg cgctcgccga gctgcacggc ggtgtttgcg gcgcgttatg tgcgggcggc
     1381 gctcccgcgg cgcgtcgttg gctcgaggat tgcctcgacg atcaggagct cgcaacctcg
     1441 acggcggccg tcgccgaaga gctcgaggag ctgatcggcg ccagcgaggc cacgcttgcg
     1501 aatcccgatc tgaaattcga gcccttgtta cccgacgacg acgcgccgct cgacgagcag
     1561 gtgcaggcac tcgcgctgtg gtgccaaggc ttcttgagcg gcgtcggcac gacggcgccg
     1621 cgcgccgcgc taccgagcga cgaaggcagc tcgctcgccg agatcctgcg cgacttcgcc
     1681 gagatcagcc gcgccggatt gagcgaggag gaggccgccg gccaggcgca ggccgacttc
     1741 gcgctcgcgg agatccacga attcgtgcgc gtcagcgtgg cagtagtgtt cgaagagctc
     1801 gctgcgctgc gggctgccgc tacgcgcgac atacactagc ggctgagctc gacgccagag
     1861 gtcatagcaa cgtgaatccc aaggaattcg cgcgccgccg caggcagctc atggagctca
     1921 tcggcacgag cggcatcgcc atcctcgctg ccgcgcccga gcgcgttcgc agccgcgaca
     1981 ccttgtacgt gtaccgccaa gacagcgact tctattacct gacggggttc ggcgagcccg
     2041 aagccgtcgc cgtgctcgtg ccgggtcgcc cgcagggcga attcctgatg ttctgccgcg
     2101 agcgcaatcc cgagcgtgag cagtgggacg gcccgcgagc aggccccgaa ggcgctgtcg
     2161 cggcttacgg cgccgacgat gcgttcccga tctcggacgt ggacgacatc ctgccgggcc
     2221 tgctcgagcg cagcgaccgt gtctactatt cgatcggcgg caacgagttc gatcagaagc
     2281 tccttgcgtt catccaggcg ttgaactcga agcggcagag cgggcacgcg ccgagcgagc
     2341 tcgtcgcgct cgatcatcta ttacacgaga tgcggttgtt caagagtcgc gccgagacgg
     2401 gcgtgatgcg ccaagccgcg cgcatcgcag tcagcgcgca cagccgcgcg atgcgcgcct
     2461 gccagccggg gctcaaggag tacgagctcg aggccgagtt cgtgcacgag ttccgcaggc
     2521 acggcgccga atgctcgtac ccgccgatcg tcgcggccgg cgtcaacgcc tgcatcttgc
     2581 actaccgctc gaacgatgcg ccgttgctcg acggcgatct ggtgctcatc gacgccggct
     2641 gcgagtacgg catgtacgcc tccgatgtca cgcgcacgtt ccccgtgaat ggccggttca
     2701 gcccggcgca gcgcgagctc tatgaggtcg tgctcgaggc caaccaagcc gcgaccgcga
     2761 aggcgatgcc cggcaatcac tggaatgagc cgcacgatgc ggccgtcggc gtcgtgacgc
     2821 ggggtttgaa ggacctcggt ttgctcgccg gccgcgtgcc gcagctcgtg aaggccatgg
     2881 cctaccggca attcttcatg cacaagaccg gccactggct cggcctcgac gttcacgacg
     2941 tcggcgacta caaggtcgcc gatcagtggc ggttgctcga gccgggaatg gtcatgacga
     3001 tcgagcccgg cgtctacgtc gccgcgaacg acaagagcgt cgacaagcgc tggcgcggca
     3061 tcggcatccg catcgaggac gacgtgcagg tgacgcgcga ggagcccgag gtgctgaccc
     3121 aggggttgcc gacgacggtc gcggagatcg agcggcacat gggcagcgcc gcgtgaacgg
     3181 tcaaggccgc gactgcgacg tcctcattgc aggaggcggc gcggtcggct cggcgctcgc
     3241 gtgcgcgctc gcggagctgc cgttgacggt cgtgctcgtc gaggcgcagc aggtgcgatt
     3301 gctcgagcag ccgagcttcg acgcgcgcgt cacggcgctc gccaacggct cgcagcagat
     3361 actcgcgggg ctcgatctct ggcccgagct caagggctac gcacaggcga ttcgctcgat
     3421 tcatatctcg gagcgcggcc ggttcggcgc cgcgcgtatc gatgcacgag aagagcatgt
     3481 gccggcgctc ggctacacga tcgagaacct cgtgctcggc cgcgtgctct gggagcggtt
     3541 gcagcgcgcg ccgcggttga ccgtgctggc gcctgcgaag gtcacggacg tcgcgcgcgt
     3601 tcccgagcac gcgctcgtct cgctgcagca cggcgctgcc gtcgagacgg tccgcgccaa
     3661 gctcgtcgtc gcggccgatg gcgtgcgctc ggccgtgcgc gcggcgctcg gcgtcgaaac
     3721 gagcgagcac gactacgctc agcgtgccgt catcttcaat tgcagcaccg aggcgccgct
     3781 cgagggccgc gcgtacgaac ggttcactgc gcgcgggccg atcgcgttgc tgccgctcgc
     3841 gggcggccgc gcggccgtga tctggacgct gcccgaaggc gacgcccagc gcaccgcggc
     3901 gctctccgcc gacgacttcc gcgcggagct gcaagcggcg ttcggccaac gtctcggacg
     3961 ctttacgaga attggcgagc gtcacctcta ttcgctcgcg cgggtcgcca gcggcgccat
     4021 ccacggcgag cgcgccgtgc tgatcggcga cgcggcgttg cggctgcatc ccgtcgcagg
     4081 gcagggcttc aacctcgcgc tgcgcgacgt cgcgacgttg gcggaggtca tcgccgacgc
     4141 gctgcgcgct gcgcgtacgc tgggctcggc cgtcgacgtc ggcgccgcgg agctgctcga
     4201 acgctacgcg gcctggcgtg cctcggaccg gcaacgagtc tcgtcgttca cgcacggctt
     4261 gatccagctg ttcggcgaat cggcgcccgg tatcggcctc ggccgcggct tgggcctcat
     4321 ggccttcgat ttgttgcccg gcgcgaaagc gctgctcgcg cgccagacga tgggcaaggc
     4381 cggacgcctg ccgcgcttgg cgcgcgggct caagcttagc tagagcgcag atggcgaaca
     4441 cacccaccgt gctcgtggcc ggcgcaggcg tcgtcgggct cgcggctgcc gcattgctgg
     4501 ccacgggccg ctgcgccgat cgcctgcgcg tgctcgtgct cgacgcgcgt cccctcct
//
LOCUS       DATLEE010000648         5707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8636166, whole genome shotgun sequence.
ACCESSION   DATLEE010000648 DATLEE010000000
VERSION     DATLEE010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8636166"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /gene="mscL"
                     /locus_tag="VM692_14195"
     CDS             <1..342
                     /gene="mscL"
                     /locus_tag="VM692_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HUQ53373.1"
                     /translation="KIVTSLVNDIVMPPIGLLMGNVDFSNLFVNLTMGATYASVAEAE
                     AAGAPIVKYGMFINTVLDFIIVAFAIFMVIRGLNRLRRKQEAAPPAVPPAPTPDQKLL
                     TEIRDLLKQGR"
     gene            complement(420..1148)
                     /locus_tag="VM692_14200"
     CDS             complement(420..1148)
                     /locus_tag="VM692_14200"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HUQ53374.1"
                     /translation="MTDGSKIVVSGRHVMLVAAERAEAPRPDWFDPAEWRRAGAVAIE
                     TSGRGEVLIVAHGPETWVLRHYRRGGLVARLIDDHYVWFGAESTRAFREWRLLRKLRA
                     AGLPVPNPVAARIYRTGVIYTADIITTYLPATHKLSWYIEQGGAPADCWQRIGAMIRA
                     VHDHGVDHPDLTAHNVLLDETGKTFLVDFDNAQLKPPGDWQRLGLERFNRSLRKVALE
                     TGTDFDAAAWREIEAGYGPLRGGR"
     gene            complement(1145..2197)
                     /gene="waaF"
                     /locus_tag="VM692_14205"
     CDS             complement(1145..2197)
                     /gene="waaF"
                     /locus_tag="VM692_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HUQ53375.1"
                     /translation="MTAPDKVLVVGPSWVGDMVMAQALYKLLKRESPTAEIHVVAPPW
                     SLPVLERMPEVARGVELGVGHGELAFGRRRSLGRRLRAERYTRAIVLPRSAKAALVPW
                     FAKVPLRTGFRGEWRYGLLNDVRGLDARLDQTVKRFVALGQPRGAPPPQALPPELRPQ
                     LRSDAANLARLRAAHSLRSGAPLVALMPGAEYGPAKRWPAAQYGALAAELAVADRDVV
                     VLGSAKERAIGDEVVARAAQPSVHNLCGATSLADVVDLSAAAEVAISNDSGLLHVAAA
                     AGAPVVAIYGSSSPQFTPPLTPSAAIVSLALECSPCFARDCPLRHMRCLNDLPPATVL
                     RAVESVRGQRARSGGR"
     gene            complement(2194..3159)
                     /gene="rfaD"
                     /locus_tag="VM692_14210"
     CDS             complement(2194..3159)
                     /gene="rfaD"
                     /locus_tag="VM692_14210"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017377933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HUQ53376.1"
                     /translation="MIVVTGGAGFIGSNLVRELNRRGHDDVVVVDDLSDGRKFVNLVD
                     CRIADYFDKSEFVARLKDDELGKIEAIFHQGACAVTTEWNGRFMMEANYRYSVDVYEY
                     CVAKRVPLIYASSAAVYGASSTFKESDTAAERPLNVYGYSKLLFDNYVRRGLTKRGRA
                     PRGRAPVVGLRYFNVYGPGEAHKGPMASVAFHLHGQVAATGHARLFEGSGGYAAGEQR
                     RDFIYVGDVVDVNLWFYERRKASGVFNVGTGASATFNDVASAVIAWHGRGAIRYIPFP
                     DDLKSRYQSFTEADITALRKAGYARPFRDVTVGVKDYLDALGARP"
     gene            complement(3176..3397)
                     /locus_tag="VM692_14215"
     CDS             complement(3176..3397)
                     /locus_tag="VM692_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019557721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HUQ53377.1"
                     /translation="MRERHGRVQESLTTANAERVYNVTRAELPLHCPLPSMYLWNSHP
                     KVYLPIEATGEAKCPYCSTEYRLAEDEAR"
     gene            complement(3447..>5707)
                     /gene="glnE"
                     /locus_tag="VM692_14220"
     CDS             complement(3447..>5707)
                     /gene="glnE"
                     /locus_tag="VM692_14220"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HUQ53378.1"
                     /translation="EDGFAYRIDTRLRPFGASGPLAVSLAALEAYLVQHGRDWERYAY
                     VKARLLTGQSFAPEIFDPMLTPFVYRRYLDYGVFDALRQMKRLISQEVARKDMGDNIK
                     LGPGGIREIEFIAQALQIVRGGRRPELRTRSLLEVLPRLARDRQLGDSTVAALATAYR
                     YLRTVENRLQAFDDQQTHVLPTEPEARAVLAYSLGEPTWATFAAHLESQRALVEAEFE
                     RVAWEAQGAAPRDDDPVTSAWAAGDAAAMLANTPLAHEERALELLKDLRHGGLYQRMD
                     EVGRQRLAAVMVRTLSVLAAHPAALKSLERVLPVFRAVGRRSAYLALLNENPAALDRL
                     LKLVVDSAWLARQIAEQPLLLDELLDNRVFETPPSREELAALLERLTEGVGEGDVEAM
                     LDAIRVFQRTAIFRIAVADRSGSLPLMKVSDRLTDTAELVLDYSLRAARRELVAKYGT
                     PRCGPPPPVGDYQGASPVGDYQGALREAGFAVIGYGKLAGLELGYGSDLDLVFLHDSS
                     GAQQETDGSPPVDNERFFGRLVQRLIHFLSVQTSSGRLYEVDTRLRPSGRSGLLVTSL
                     EGFRHYQAEDAWTWEHQALLRSRALAGSEAVCRAFEAIRHDILVAHVDRASLKSEVAK
                     MRGRMRSELSLAKEGSFDIKQDPGGIADVEFLVDYWVLAHSAEYPELIEFPDNIRQLE
                     ALERVGLVPAERCRRLKECYLELRQRVHELALDEGGRVVPEHEFTAVREFVRTVWHEV
                     FGDVEGADSESD"
BASE COUNT          969 a         1952 c         1877 g          909 t
ORIGIN      
        1 aagatcgtaa cgtccctcgt caacgacatc gtcatgccgc cgatcggtct tctcatgggc
       61 aacgtcgatt tcagcaacct gttcgtgaac ctgacgatgg gcgcgactta cgccagcgtc
      121 gcggaagccg aggccgcagg agcgccgatc gtgaagtacg gaatgttcat caacaccgtg
      181 ctcgatttca tcatcgtcgc gttcgcgatc ttcatggtca ttcgcggctt gaacaggctg
      241 cggcgcaagc aggaagctgc gccccccgcc gtgccgccgg ctcccacgcc cgaccaaaag
      301 ctgctgacgg agatccgcga cctattgaag cagggccggt agcagcacgg acgcttcgcg
      361 ggccgcgatt tgcatccttg cgagcacggc acttccgccc tcccccggcg gccggcccct
      421 taccgacccc cgcgaagcgg gccgtagccg gcctcgatct cgcgccatgc agcggcgtcg
      481 aagtcggtgc cggtttcgag cgcgactttg cgcagcgaac ggttgaaccg ctcgagcccc
      541 aagcgctgcc aatcaccggg cggctttaac tgcgcattgt cgaagtcgac gaggaacgtc
      601 ttccccgtct cgtcgagcag cacgttgtgc gccgtcagat cggggtgatc gacgccatga
      661 tcgtgaacgg cgcgaatcat cgcgccgatg cgctgccaac agtctgccgg ggcgccgccc
      721 tgctcgatgt accacgacag cttgtgcgta gcgggtagat aagtcgtgat gatgtcggcc
      781 gtgtagatca ctcccgtgcg atagatacga gccgccacgg ggttcgggac cggcaagcca
      841 gcggcgcgca gcttgcgcag cagccgccat tcacgaaaag ctcgcgtgct ctctgcgccg
      901 aaccagacgt agtgatcgtc gatcaagcgc gcgacgagac cgcctcggcg atagtggcgc
      961 aggacccacg tttccggccc atgcgcgacg atcagcacct cgccccggcc cgacgtttcg
     1021 atcgcgacgg cgccggcacg ccgccactcc gctggatcga accaatctgg ccgcggcgct
     1081 tcggcgcgct cggccgcgac gagcatgacg tgccgtccgc ttacgacgat cttgctgcca
     1141 tccgtcaccg gccgccgctc cgcgcgcgtt gcccgcgcac cgactctacg gcgcgcagca
     1201 cggtcgccgg cggaaggtca ttcaggcagc gcatgtgccg caacgggcag tcgcgtgcga
     1261 agcaagggct gcactcgagc gcgagcgaca cgatggctgc gctcggcgtc aacggcggcg
     1321 tgaactgcgg cgacgacgag ccgtagatcg cgacgacggg ggcaccggca gcggcggcaa
     1381 cgtgcaacaa gccggagtcg ttgctgatcg cgacctccgc cgctgccgag agatctacga
     1441 cgtcggccag cgaagtggca ccgcagaggt tgtgcacgga cggctgcgcg gcgcgagcga
     1501 cgacctcgtc gccgatcgca cgctccttgg ccgagcccag cacgacgacg tcgcgatcgg
     1561 cgaccgcgag ctcggcggcc aacgcgccgt actgcgcagc gggccagcgc ttcgcgggac
     1621 cgtattcggc gccgggcatc agcgcgacta gcggcgcgcc agagcgcagc gagtgcgcgg
     1681 cgcgcaaccg cgcgagattg gccgcatcgc tgcgaagctg cggccgcaat tccggcggca
     1741 gcgcctgcgg gggtggcgca ccgcgcggtt gcccaagggc cacgaagcgt ttgaccgttt
     1801 gatcgaggcg ggcatcgagc cctcgcacgt cgttcagcaa cccgtaacgc cactcgccgc
     1861 gaaagcccgt gcgcaatggc acctttgcga accacgggac gagcgccgcc ttcgcagagc
     1921 gcggcagcac gatcgcgcgc gtatagcgct cggcgcgcag ccgccgcccc agcgagcgcc
     1981 ggcggccgaa agcgagctcg ccgtgaccga cgccgagctc gacgccgcgt gcgacctcgg
     2041 gcattcgctc gagcacgggc agtgaccagg gcggggcgac aacgtgaatc tcggctgtcg
     2101 ggctctcgcg cttcaacaac ttatagagcg cctgcgccat caccatgtcg cccacccacg
     2161 aagggccgac gacgagcact ttgtccggcg ctgtcacggc cgcgcgccga gcgcgtcgag
     2221 atagtccttg accccgacgg tcacgtcgcg gaacggccgc gcgtagccgg ctttgcgcag
     2281 cgcagtgata tcggcttcgg tgaagctctg gtagcgcgac ttcaagtcgt ccggaaacgg
     2341 gatgtaacgg atcgcgcctc tgccgtgcca ggcgatgacc gcgctcgcga cgtcgttgaa
     2401 cgtcgcgctc gcacccgtgc cgacgttgaa cacgccggag gctttgcgcc gctcgtagaa
     2461 ccacaaattg acgtcgacga cgtcgccgac gtagatgaag tcgcgccgct gctcgccggc
     2521 cgcgtaaccg ccgctgcctt cgaacaagcg cgcgtgcccc gtcgcggcaa cctgaccgtg
     2581 caaatgaaac gcgacgctgg ccatcgggcc cttgtgcgcc tcgccgggcc cgtagacgtt
     2641 gaagtaccgc aggccgacga ccggagcacg acctctaggc gctcggccgc gcttggttag
     2701 cccacgccga acgtagttgt cgaacaacag cttcgaatag ccgtagacgt tcaacggccg
     2761 ctccgccgcg gtgtcgctct ctttgaatgt ggagctcgca ccgtagaccg ccgccgacga
     2821 cgcgtagatc agcggcacac gcttcgcaac gcagtactcg tagacgtcga ccgagtagcg
     2881 ataattggct tccatcatga agcgcccgtt ccactccgtg gtgacggcgc aagctccttg
     2941 atgaaatatc gcctcgatct tgcccagctc gtcgtccttg agccgcgcaa cgaactcgga
     3001 tttgtcgaag tagtcggcga ttcggcagtc gacgaggttc acgaacttgc gtccgtcgct
     3061 gagatcgtcg acgacgacga cgtcgtcatg accgcggcga ttcagctcgc ggaccaggtt
     3121 gctaccaatg aaaccggcgc ctccggtgac gacgatcatg gcaacgaaga gcccgctacc
     3181 gcgcctcgtc ttcggccaag cgatattcgg tgctgcagta cgggcatttc gcctcgcccg
     3241 tcgcttcgat cggcagatag acctttggat gcgagttcca caggtacatg ctcggcagcg
     3301 ggcagtgcag cggcagctcg gcacgcgtga cgttgtaaac gcgttcggcg ttggccgtcg
     3361 tcaagctctc ctgcacccta ccgtgacgtt cgcgcactgc ctgctctcct gctccgcgag
     3421 gcggggatta tacccgcggc agctggctag tcgctttcgg agtcggcgcc ttcgacgtcg
     3481 ccgaacactt cgtgccagac ggtgcgtacg aactcgcgaa cggccgtgaa ctcgtgctcc
     3541 ggcacgacgc ggccgccttc gtcgagcgcc agctcgtgaa cgcgttgccg tagctcgaga
     3601 tagcactcct tgagacgccg gcaccgttcc gccggcacga gaccgacgcg ctcgagcgcc
     3661 tcgagctggc gaatgttgtc gggaaactcg atgagctcgg gatactcggc cgaatgcgcc
     3721 agtacccagt agtcgacgag gaactcgacg tcggcgatgc cgccgggatc ttgcttgatg
     3781 tcgaaagagc cttccttcgc cagcgagagc tccgagcgca ttcggccgcg catcttcgcg
     3841 acctcgctct tgaggctcgc ccgatcgaca tgcgcgacga ggatgtcgtg ccgaatcgcc
     3901 tcgaacgcac ggcaaacggc ttcggacccc gcgagcgccc ggctgcgcag cagggcttgg
     3961 tgctcccacg tccaggcgtc ttccgcctgg taatgacgaa atccttcgag gctcgtgacg
     4021 agcaagccgg acctgccgct cggccgcagc cgcgtgtcga cctcgtagag acgtccggag
     4081 ctcgtctgca cggaaagaaa atgaatcagg cgctgcacga gccggccgaa gaagcgctcg
     4141 ttgtcgacgg gcgggctgcc gtccgtttct tgctgggcgc cgctcgagtc gtgcaggaag
     4201 acgaggtcga gatccgagcc gtagccgagc tcgagccccg ccagtttgcc gtaaccgatg
     4261 acggcgaagc cggcttcgcg caacgcccct tggtagtccc cgacggggga cgccccttgg
     4321 tagtccccga ccgggggcgg cgggccgcag cgcggagtgc cgtatttcgc gacgagctcg
     4381 cgtcgcgcgg cgcgcagcga atagtcgagc acgagctcgg cggtatcggt gagccggtcg
     4441 ctgactttca tgagtggcaa cgagcccgac cggtccgcga cggcgatgcg gaagatcgcc
     4501 gtgcgctgaa atacccggat cgcgtcgagc atcgcttcga cgtcgccttc gccgacccct
     4561 tcggtgaggc gctcgagcag cgcggcgagc tcctcgcgcg acggcggcgt ttcgaagacg
     4621 cggttgtcga gcagctcgtc gagtagcagc ggctgctcgg cgatctgtcg cgccagccac
     4681 gcgctatcga cgacgagctt caacaagcgg tcgagcgccg cggggttctc gttgagcagc
     4741 gccaggtacg cgctgcggcg accgacggcg cggaacacgg gcaacacgcg ctcgagcgac
     4801 ttcagcgccg ctgggtgcgc ggccagcacc gagagcgtgc gtaccatgac tgccgcaaga
     4861 cgctgacgcc cgacctcgtc catgcgttga tagaggccgc cgtggcgcag gtccttcaac
     4921 agctcgagcg cgcgttcttc gtgcgcgagc ggagtattcg ccagcatggc cgcggcgtcg
     4981 ccggccgccc aggcgctcgt gacgggatcg tcgtcgcgcg gcgcggcacc ctgcgcctcc
     5041 caggcgacgc gctcgaactc cgcctcgacg agcgcccgct gcgattcgag atgcgccgcg
     5101 aaggtcgccc aagtcggctc gccgagcgag tacgccaaga ctgcgcgcgc ttcgggctcc
     5161 gtgggcaaca cgtgcgtctg ctggtcgtcg aacgcctgca ggcggttctc gaccgtgcgc
     5221 agatagcgat acgccgtcgc gagcgcagcg accgtgctat cgccgagctg tcgatcgcgc
     5281 gcgagccgcg gcaagacctc gagcaacgag cgagtgcgca gctccggccg ccggccgccg
     5341 cgcacgatct gcaatgcttg cgcgatgaac tcgatctcgc ggatgccgcc cggcccgagc
     5401 ttgatgttgt cgcccatgtc cttgcgcgca acttcttggc tgatgagccg cttcatctgt
     5461 cgcagcgcgt cgaatacgcc gtaatcgagg taacgtcggt acacgaacgg cgtcagcatg
     5521 ggatcgaaga tttccggcgc gaacgactgc cccgtcagca accgagcttt gacgtaggcg
     5581 tagcgctccc aatcgcggcc gtgctgcacg aggtacgcct cgagcgccgc gaggctgacc
     5641 gcgagcggac cgctggctcc gaacgggcgt aggcgcgtgt cgattcgata agcgaaaccg
     5701 tcctcgg
//
LOCUS       DATLEE010000649         2002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8636827, whole genome shotgun sequence.
ACCESSION   DATLEE010000649 DATLEE010000000
VERSION     DATLEE010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8636827"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VM692_14225"
     CDS             <1..619
                     /locus_tag="VM692_14225"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUQ53379.1"
                     /translation="WWIVQSGSLKFTVEGREPVVATKGFMVQVPYRTLYQIENVGTEA
                     ALRFEVNVTRARKLYPMDETPTPVPGFDFVPTRVTGGRGTLDADNRPVVDFNEVVAGK
                     ERGGTFVDDDRSFANIIIGNYQRPQPGNKGHFHEEGSEFWLIMLGKIRYNIEGLPEFE
                     AEPGDVVYVPRQTWHLASFGGSDGLESCRLAMNGFPYQAHMFETD"
     gene            635..>2002
                     /locus_tag="VM692_14230"
     CDS             635..>2002
                     /locus_tag="VM692_14230"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUQ53380.1"
                     /translation="MTEKTKITLDRRGFFRGAAGLAVGGFGATTALAQTGATTAGAPA
                     SADAAKQLEEQTQSAVRWAGREPADWVIPRNGADHNVVIVGGGQSGLSIAYGLKRKGV
                     GRVEVIDRAAPGQAGIWRNIARMHQLRTPKTLAGPELGNPALSFRAWYEALHGTAAFD
                     ALDRIPRLAWADYLDWFQKTTGTTVRYGTRLVEIEPQGDLLRLHLEQDGAQRTVTTRK
                     VVLANGYAGAGGPNVPEFIRALPANLWTHTTGNIPFAALKGKVVGVVGAGSSAFDAAA
                     VALESGASEVHLFSRRSYIDYQGQPAPAPGATPPPPPAPPPARGYGNMLELSYELPDV
                     VRWRNFLLGDRRVASVPLDSLQRAVALKGFEIHLDSSLSDVAASGNKVTARVGSKRFR
                     FDHLIAATGYRIDLPLQPELARIQQSISLWGDRFKPAAGEESGAGAAHPYLGAGFEFL
                     PKSGVD"
BASE COUNT          339 a          688 c          674 g          301 t
ORIGIN      
        1 atggtggatc gtgcagtccg gctcgctgaa gttcacggtc gaaggccgcg agcccgttgt
       61 cgcgacgaaa ggcttcatgg tgcaggtgcc gtatcgcacg ctgtatcaga tcgagaacgt
      121 cggcaccgag gctgcgctgc gctttgaggt caacgtgacg cgcgcgcgca agctgtaccc
      181 gatggacgag acgccgacgc cggtgccggg cttcgacttc gtgccgacgc gcgtgacggg
      241 cgggcgcggc acgctcgacg ccgataaccg gcccgtcgtc gacttcaacg aggtcgtcgc
      301 cggcaaagag cgcggcggca ccttcgtcga cgacgaccgc agcttcgcca acatcatcat
      361 cggcaactat cagcgcccgc agccgggcaa taagggccac ttccacgagg aagggtcgga
      421 gttctggctg atcatgctcg gcaagattcg ctacaacatc gaaggcctgc cggagttcga
      481 agccgagccc ggcgacgtcg tctacgtgcc gcgccagacg tggcacctcg cgagcttcgg
      541 cggcagtgac gggctcgagt cctgccgtct cgcgatgaac ggctttccgt atcaggcgca
      601 catgttcgag acggactagt tcgaggggaa cgacatgaca gaaaaaacca agatcacact
      661 cgatcggcgc ggcttcttcc gcggcgcggc cgggctcgcc gtcggcggct tcggcgccac
      721 gacggcgctc gcgcaaacag gggcgacgac cgccggcgcg cctgcaagcg ccgatgctgc
      781 caagcagctc gaggagcaga cgcaaagcgc ggtgcgctgg gccgggcgcg agcccgccga
      841 ttgggtgatc ccgcgcaacg gcgcagacca caacgtcgtc atcgtcggcg gcggccagag
      901 cggcttgtcg atcgcctacg gcctgaagcg caaaggcgtc ggccgcgtcg aggtcatcga
      961 tcgcgccgcg ccgggacaag cgggaatctg gcgcaacatc gcacgcatgc atcaattgcg
     1021 cacgccgaaa acccttgcag gcccagagct cggcaacccc gcgctgagct ttcgcgcctg
     1081 gtacgaagcg ctgcacggca cggccgcgtt cgacgcgctc gatcgcattc cgcgcctcgc
     1141 ctgggccgac tatctcgact ggtttcagaa gacgaccggc acgacggtgc gctatggcac
     1201 gcgactcgtc gaaatcgagc cgcagggcga cctgctgcgc ctgcacctcg agcaagacgg
     1261 cgcgcagcgc acggtgacga cgcgcaaagt cgtgctcgcg aacggctacg ccggtgccgg
     1321 cggccccaac gtgccggagt tcatccgcgc gctgcccgcg aatctctgga cgcacacgac
     1381 gggcaacatt ccgttcgcgg cgctgaaggg caaagtcgtc ggcgtcgtcg gcgcggggtc
     1441 gtcggcgttc gacgcggccg ccgtggcgct cgagtcgggc gcgtcggagg tgcacctctt
     1501 cagccgccgt tcgtatatcg actatcaagg gcaacctgcg ccggcgccgg gtgccacacc
     1561 gccgccgcca ccagcgccgc cgccggcccg cggctacggc aatatgctcg agctttcgta
     1621 cgagctgccc gacgtcgtgc gctggcgcaa cttcttgctc ggcgaccgcc gcgtcgcgtc
     1681 ggtgccgctc gactcgctgc agcgcgccgt cgcgctcaag gggtttgaga tccatctcga
     1741 cagctccttg tcggacgtcg ccgcgagcgg caacaaagtc acggcccgcg tcggcagcaa
     1801 gcgcttccgc ttcgaccacc tcattgccgc cacgggctac cgcatcgatc tgccgttgca
     1861 accggagctc gcgcggattc agcaatcgat ctcactgtgg ggcgaccgct tcaagccggc
     1921 tgccggagag gaaagcggcg cgggcgcggc gcatccgtat ctcggcgcgg gcttcgagtt
     1981 cttgccgaag agcggcgtcg ac
//
LOCUS       DATLEE010000650         5245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3437507, whole genome shotgun sequence.
ACCESSION   DATLEE010000650 DATLEE010000000
VERSION     DATLEE010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3437507"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VM692_14235"
     CDS             <1..1258
                     /locus_tag="VM692_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HUQ53381.1"
                     /translation="QLSNFARSLSVETAFTVLAIARSLKDAGKDVVELEIGDSPFDST
                     AAARAGGIEAIKNNQSHYCPSPGIPEFRAAAAEFVRDEFGVPAEASNIVAGAGAKVFQ
                     QFFCEAFLNPGDGVLVFSPYFPTFVPNIERRGARAVLTPLKQANGFRPTVADIERFLE
                     HDASPRAIFLNSPHNPTGGVTTKDDLTRIADLVRGRDVAVFSDEPYCHMVWRGNHHSI
                     LSEPGMLAQCVGAYTFSKSYSMSGWRLGFAVASVEVAEVIGKMINTTLSCTPPIVQLA
                     GTAALQRDKQERDAVMQKFREKVVLLTEGLNRIEGFRALDPTATFYVFPNVMRVCNDL
                     GVTSHGLALYMLQGADDQFGIACLGGECFGEAGAGFLRFSCAESNERLQRALEFIPKA
                     VARRDRLDAWLAKNPQYRLRTPYPVE"
     gene            complement(1458..2867)
                     /locus_tag="VM692_14240"
     CDS             complement(1458..2867)
                     /locus_tag="VM692_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HUQ53382.1"
                     /translation="MSEIVFAPARDLAARLAARELSAVEVMRAFLAQIERVNPQVNAI
                     VTLRPAEELLADAAAADRKLASGEAVGALHGLPIAIKDLSLTRGLRTTFGSTLYRNFV
                     PTSDDLYVERVRRAGAIVIGKTNTPEFGAGSQTFNALFGATRNPYDVGKTCGGSSGGG
                     AVALACGMLPLADGTDLGGSLRNPASFCNVVGFRPSPGRVPRLHARAGDTLGVHGPMA
                     RDVADLALLLSVVAGPDPRDSQSLPEPGLVFRDVGERDFAGARVAWSENLGRYPVEPA
                     VTAVCNAARSVFAELGCDVRDDSPNLDGVDELFQTQRAHGYAVALARDLERGRSQLKD
                     TVVWNIEQGLKLTPADLERAERLQAALDARVAAFFATHEFLVLPTVQVLPFPVEIDWV
                     RAIDGVAMQTYIDWMASCYAISCLKTPAISVPCGFSPEGLPVGLQIVGGPGRDRRVLQ
                     LARAFEQATQHARRRPASV"
     gene            complement(2893..4575)
                     /locus_tag="VM692_14245"
     CDS             complement(2893..4575)
                     /locus_tag="VM692_14245"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HUQ53383.1"
                     /translation="MTDFDYRKYRAYRPITLSDRQWPSRTIERAPDWCSVDLRDGNQA
                     LVQPMSVAKKLEMFALLVRLGFKEIEIGFPSASQTEYDFARRLIDKHLIPDDVTVQVL
                     TQARRELIERTFESLRGIKRAVVHVYNSTSPVQREQVFKAGKQEIITIAVNGARLLHE
                     GAARYPGVDWTFEYSPESFSGTEPDYAVEVCNAVIDVWQPQRGQKVVINLPATVEMTT
                     SNVFADQVEHFCRNVAQREHIRVSLHTHNDRGCAVAAAELGLLARADRIEGTLFGNGE
                     RTGNMDIVTMAMNLYSQGIDPKLDLADMGRIIEVYKRCTDMPVHPRHPWAGDLVYAAF
                     SGSHQDAIRKSMAFHREQNLPHWGVAYLPIDPHDLGRRYEEVVRINSQSGKGGVALVL
                     ERDHGVTLPKWLHPAVSRIVQAEADATGEEIHSRQVRALFDKHFLSIPDGWRLEGYDL
                     HSEAQRTHGKFRLAMPGGDVVLAGEGQGLIEALSDAVSRRFGISVDIVEFDEHAMSAG
                     TEAKALASVTVEASGQQASACCIDEDTSLASLQATLSAIGRALAAHALRQAI"
     gene            4725..5207
                     /locus_tag="VM692_14250"
     CDS             4725..5207
                     /locus_tag="VM692_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HUQ53384.1"
                     /translation="MTAKIDAFDLKILAELQANGGLSNQELADRVGLSPSPCSRRIHQ
                     LEQAGIIRERVVRLDPRAVGLDLTVLIHIRMDRHTPERFAQFESAVRGYPEVQECFLI
                     TGQEADYQLKISVPNMDEYQRFLLNKITRIDGVIGVHSSFVLRKAVDTTALPLGYARA
                     "
BASE COUNT          876 a         1748 c         1726 g          895 t
ORIGIN      
        1 acaactcagc aatttcgctc gttcgctgtc cgtcgagacg gcgttcaccg tgctcgcaat
       61 cgcgcgctcg ttgaaggacg ccggcaaaga cgtcgtcgag ctcgagattg gcgacagccc
      121 gttcgacagc actgcggcgg cgcgggccgg cggcatcgag gcgatcaaga acaaccaatc
      181 gcactattgc ccgtcgcccg gcattcccga gtttcgcgcg gccgccgccg agttcgtgcg
      241 cgatgagttc ggcgtcccgg ccgaggcgag caatatcgtc gccggagcgg gcgccaaggt
      301 gttccagcaa tttttctgcg aagcattctt gaatcccggc gacggcgtgc tcgtgttcag
      361 cccgtacttt ccgacgttcg tgccgaacat cgagcgccgc ggcgcgcgcg ctgtgctgac
      421 gccgcttaag caagcgaacg gcttccgccc gacggtcgcc gacatcgagc gttttctcga
      481 gcacgacgcg tcaccgcgcg ccattttctt gaattcgccg cacaacccga cgggcggcgt
      541 cacgacgaag gacgacctga cgcgaatcgc ggacctcgtt cgcggccgcg acgtcgccgt
      601 gttcagcgac gagccgtatt gccacatggt ctggcgcggc aaccatcatt cgatcctgag
      661 cgagcccggc atgctcgcgc agtgcgtggg cgcttacacg ttcagcaagt cgtacagcat
      721 gagcggttgg cgcctcggct tcgccgtcgc gtcggtcgaa gtcgccgagg tcatcggcaa
      781 gatgatcaac acgacgttgt cgtgcacgcc gccgatcgtg cagctggcag gcaccgccgc
      841 gttgcaacgc gacaagcaag agcgcgacgc ggtcatgcag aagtttcgcg agaaggtcgt
      901 gctgctgacc gagggcctga accggatcga agggttccgc gcgctcgacc cgacggcgac
      961 gttctacgtg tttccgaacg tcatgcgcgt ttgcaacgac ctcggcgtca cgagccacgg
     1021 cttggcgctc tacatgctgc aaggcgccga cgaccaattc ggcatcgcgt gcttgggcgg
     1081 cgaatgcttc ggcgaggccg gcgccggttt tctgcggttc agctgcgccg aatcgaacga
     1141 gcgcctgcaa cgcgcgctcg aattcattcc gaaggccgtt gcgcgccgcg accggctcga
     1201 tgcctggctc gccaagaacc cgcagtaccg cctgcgaacg ccctaccccg tcgaataaca
     1261 gcgccggcgt cggccgcttc gcttatgtca aacctgtgac gccgttcaca ggctctttcc
     1321 atttgtggat ggatactgac gtccaggttc ggagattgtt gctcctgaac ggtttccccc
     1381 taactcgccc ggccttcccg ccgggctttt ttttgcgcta ggcgcgcgat gaagccgacg
     1441 agcagcaaac ggcgacgcta gacgctcgcg ggccgtcgcc gcgcgtgctg cgttgcctgc
     1501 tcgaaggcgc gagcgagctg caacacgcga cgatcgcggc ccgggccgcc gacgatctgt
     1561 aggccgaccg gcaggccttc aggcgaaaac ccgcacggca cgctgatggc cggcgttttg
     1621 aggcagctga tcgcgtagca gctcgccatc caatcgatat aggtctgcat cgcgacgccg
     1681 tcgatggcgc gcacccagtc gatctcgacg gggaacggca agacctgcac ggtcggcaac
     1741 acgagaaact cgtgcgtggc gaaaaaagca gcgacgcgcg cgtcgagagc ggcttggagt
     1801 cgctccgcgc gctcgagatc ggccggcgtg agcttgaggc cctgctcgat gttccagacg
     1861 acggtgtcct tgagctgcga gcgcccgcgc tcgaggtcgc gcgcgagcgc gacggcatag
     1921 ccgtgcgcgc gctgcgtttg gaacagctcg tcgactccgt ccaggttcgg cgagtcgtca
     1981 cgcacgtcgc agccgagctc ggcgaacacc gagcgcgccg cgttgcagac cgcggtcacg
     2041 gcaggctcga ctggatagcg cccgaggttt tcgctccacg cgacgcgcgc gccggcgaag
     2101 tcgcgttcgc cgacgtcgcg gaacacgagg ccaggctccg gcagcgattg cgagtcgcgc
     2161 ggatcgggcc cggccacgac ggacagcaac agcgcgaggt ccgcgacgtc gcgcgccatc
     2221 ggcccgtgca caccgagcgt gtcgccggcg cgcgcatgca ggcgcggcac gcggcccggc
     2281 gacggacgaa agccgacgac gttgcaaaag ctcgcaggat tgcgtaacga gccgccgagg
     2341 tcggtgccgt cggcgagcgg cagcattccg caagcgagcg cgacggcgcc gccgccgctg
     2401 ctgccgccgc aagtcttgcc aacgtcgtac ggattgcgcg tcgcaccgaa cagcgcgttg
     2461 aacgtttgcg agcccgcgcc gaactccggc gtgttcgtct tgccgatgac gatcgcgccg
     2521 gcgcggcgca cgcgctcgac gtagagatcg tccgacgtcg gcacgaaatt acgatagagc
     2581 gtcgagccga acgtcgtgcg cagaccgcgc gtcagcgaga ggtccttgat tgcgatcggc
     2641 agcccgtgca gtgcgccgac cgcttcgccg gaggcgagtt tgcgatccgc ggcggcagcg
     2701 tcggccagca actcctcggc ggggcgcagc gtcacgatcg cattgacctg cggattgacg
     2761 cgctcgatct gcgccagaaa cgctcgcatg acctcgacgg ccgagagctc gcgcgccgcg
     2821 agccgcgcgg ccaggtcgcg cgccggggcg aacacgatct cgctcacgct cgagcccgct
     2881 ggcaaaacgc cttcagatcg cttgccgcag cgcgtgcgcc gcgagcgcgc ggccgatcgc
     2941 cgacagcgtc gcttgcagcg aggccagcga cgtgtcctcg tcgatgcagc acgcgctcgc
     3001 ctgttggccg ctcgcttcca cggtcaccga cgcgagggcc ttcgcttccg ttccggccga
     3061 catcgcgtgc tcgtcgaact cgacgatgtc gacgctgatg ccgaagcggc ggctcacggc
     3121 gtcgctgagc gcctcgatga ggccctgccc ttcgccggcc aacacgacgt caccaccggg
     3181 catcgcgagg cggaacttgc cgtgcgtgcg ctgtgcttcc gagtgcaagt cgtagccctc
     3241 gagccgccag ccgtcgggga tgctcaggaa atgtttgtcg aacagcgcgc gcacctgccg
     3301 cgagtgaatc tcctcgcccg tcgcatcggc ctctgcctgc acgatgcggc tcacggccgg
     3361 atgcagccac ttcggcagcg tgacgccgtg atcgcgctcg agcacgagcg cgacgccgcc
     3421 cttgcccgac tggctgttga tgcgcacgac ctcctcgtag cgccggccga gatcgtgcgg
     3481 atcgatcggc agataagcga cgccccagtg cggcaggttc tgctcgcgat ggaacgccat
     3541 cgacttgcgg atcgcgtctt gatggctgcc cgagaacgcg gcgtaaacga gatcgccggc
     3601 ccacggatgg cgcggatgca cgggcatgtc cgtgcagcgt ttatagacct cgatgattcg
     3661 gcccatgtcc gctagatcga gcttcgggtc gatgccctgc gagtacaggt tcatcgccat
     3721 cgtgacgatg tccatgttgc cggtgcgctc gccgttgccg aacagcgtgc cttcgatgcg
     3781 gtccgcgcgc gccagcaagc cgagctcggc ggcggcgacg gcgcagccgc gatcgttgtg
     3841 cgtatgcagg ctcacgcgaa tgtgctcgcg ctgcgccacg ttgcgacaga agtgctcgac
     3901 ctgatccgcg aatacgttcg acgtcgtcat ctcgacggtc gcgggcaagt tgatgacgac
     3961 cttctggccg cgctgcggct gccaaacgtc gatgacggcg ttgcagactt cgacggcgta
     4021 gtcgggctcg gtgcccgaga agctctcggg cgaatactcg aacgtccaat cgacgccggg
     4081 gtaacgcgcg gcgccttcat gcagcagccg cgcaccgttg acggcgatcg tgatgatctc
     4141 ctgcttaccg gccttgaaca cctgctcgcg ttgcaccggc gaagtcgagt tgtagacgtg
     4201 cacgacggcg cgcttgatgc cgcgcagcga ctcgaacgtg cgctcgatga gctcgcgtcg
     4261 cgcttgcgtc aacacttgga ccgtgacgtc gtccggaatc aggtgcttgt cgatgagccg
     4321 gcgcgcaaag tcgtattcgg tttgcgatgc ggaagggaaa ccgatctcga tctccttgaa
     4381 gccgagccgg acgagcagcg cgaacatctc gagcttcttc gcgaccgaca tcggctgcac
     4441 gagggcctgg ttgccgtcgc gcaggtcgac cgagcaccaa tcgggcgcgc gctcgatagt
     4501 gcggctcggc cactggcgat cgctgagggt gatggggcgg taggcgcggt atttgcggta
     4561 atcgaagtcg gtcatttggc agcggtcctt cggccaggct cgagtacgta agggcccatt
     4621 ctaagcgctc gtcaggggca gaaatccgct atttctgcgg cgcggatgcg taacagcgca
     4681 atttcttgct gcttccggcg cccgcttaac gatattattg ccgaatgacg gcgaaaatcg
     4741 acgctttcga tctcaaaatc ttggccgagc tgcaggcgaa cggcgggctc agcaaccagg
     4801 agcttgccga tcgcgtcggc ttgtcgccct cgccgtgctc gcgccgaatc catcagctcg
     4861 agcaggccgg catcatccgc gagcgcgtcg tgcggctcga cccccgcgcc gtgggcctcg
     4921 acctcacggt gctgatccat atccgcatgg accgccacac gccggagcgc ttcgcacagt
     4981 tcgagagtgc cgtgcgcggc tatcccgagg tgcaagagtg ctttctgatc acggggcagg
     5041 aagccgacta ccagctcaag atcagcgtgc cgaacatgga cgagtatcag cggtttctgc
     5101 tgaacaagat cacgcgcatc gacggcgtga tcggcgtgca ctcctcgttc gtgctgcgca
     5161 aggccgtcga cacgacggcg ttgccactgg gctatgcgcg cgcctagccg ccgcggcccg
     5221 cgattcacca gtcttctttc aacag
//
LOCUS       DATLEE010000651         3226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10097530, whole genome shotgun sequence.
ACCESSION   DATLEE010000651 DATLEE010000000
VERSION     DATLEE010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10097530"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..707
                     /locus_tag="VM692_14255"
     CDS             102..707
                     /locus_tag="VM692_14255"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HUQ53385.1"
                     /translation="MTEQINDQISAFIDNELSADESALLVRRFERDTEARSRAMRYTL
                     IGSALRGELLEPHPSVLRQRLSAALSGTTTSVARTRERWTERLARPLLGLGIAATVAV
                     VAIGALRSMNDASVVSSVGAPLTTQTLPARDSYVVPVDAEESRVDAPIRLTNYLMHHG
                     EFASGLTRTSVNSNVVGATLTPAVTDAAVAKPVVVKQEGLE"
     gene            711..1670
                     /locus_tag="VM692_14260"
     CDS             711..1670
                     /locus_tag="VM692_14260"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HUQ53386.1"
                     /translation="MRVAHPVAAPSLVLLVLVGGHATAQEARDWLERMSRAVEDLNYR
                     GTFVHVLDGTAETLHIVHRNADGQSGERIISMDGAGREIVRQGDRVQGIFPDRRIVSF
                     ETRNDGSALVSALPSTTSDLEPHYDMKLGGAARVAERGVQMLEIKPRDEFRYGYMLWL
                     DQETAMPLQSQLIDEQGQVVEQILFTEIEIPAEIPAAALEPTIDTTGFTMLRAAESAP
                     LAAEIPWRAAAVPGGFTLSVATQSPMAGSDTPVEHLVYSDGLATVSVFIEDPKTKAEV
                     GEGFFSVGSTNAYSLTLDGRKVTAMGEVPRQTVRKIASSLVAQ"
     gene            1715..2104
                     /locus_tag="VM692_14265"
     CDS             1715..2104
                     /locus_tag="VM692_14265"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HUQ53387.1"
                     /translation="MVRASDREHIAVEFAAPPRCKGCGGACLWYRVPPSEELTLAASP
                     AIPVGATVAVTLPDRYLLLGAVLVYGVPLAALLAGAGTAAALFGTDLAVAAGAVLALA
                     AAVAAASFLRGRLERATLRHLAVRRVS"
     gene            2112..2348
                     /locus_tag="VM692_14270"
     CDS             2112..2348
                     /locus_tag="VM692_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HUQ53388.1"
                     /translation="MLKLTLYSRPECHLCEAFLADLMPLLGADIVVETVDVDSSVALE
                     RRYGLRIPVLAAGDVEISGYPLDQDRVRRYLESL"
     gene            2433..>3226
                     /locus_tag="VM692_14275"
     CDS             2433..>3226
                     /locus_tag="VM692_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HUQ53389.1"
                     /translation="MERIRNFSIIAHVDHGKSTLADRLIQLCGGLTAREMEDQVLDSM
                     DLERERGITIKAQSVALQYRARDGLDYQINLIDTPGHVDFSYEVSRSLAACDGALLVV
                     DAGQGVEAQSVANCYTAIEQGLEVLPVINKIDLPTADPERVIVQIEDIIGIEAKDAVR
                     ISAKTGFGVDELLERIVERIPAPKGSVDAPLQALIIDSWFDSYAGVVSLVRVMNGVIR
                     KGARIVVHSTQRKYEVERLGVFTPRSAPREELKAGEVGFLIAAIRE"
BASE COUNT          516 a         1099 c         1057 g          554 t
ORIGIN      
        1 cgtgctcgcg aagcgatcga caaggccctt gcgccattgt tgtaactgaa actgcgagca
       61 cacccttttc tcacgaccct ctttctcatg acagacagca catgacagag caaatcaacg
      121 accagatctc cgctttcatc gacaacgagc tttcggctga cgaaagtgcg ctgctcgtgc
      181 gccgcttcga gcgggacacg gaagcgcgct ctcgcgcgat gcgctacacg ctgatcggct
      241 ccgcgctccg cggggagctg ctcgagcccc atccttcggt gctgcgccag cgcttgtctg
      301 cggcgctgtc cggaaccacg acgagcgtcg cgagaacgcg cgagcgctgg accgagcgct
      361 tggcccgccc gctgctgggt ctcggcatcg cggccacggt cgccgtcgtc gccatcggcg
      421 cattgcgctc gatgaacgac gcgtccgtcg tgtcgagcgt cggcgcgccg ctgacgacgc
      481 agacgctgcc ggcacgcgat tcctacgtag tccccgtcga cgcggaggag tcgcgcgtgg
      541 acgcgccgat ccgccttacg aattatctga tgcatcacgg tgagttcgcc tcgggcttga
      601 cgcgaacctc ggtcaactcg aacgtcgtgg gcgcgaccct tacgcccgcc gtcaccgacg
      661 ccgccgttgc caagccggtc gtcgtcaagc aggaaggctt ggagtagtcg atgcgagtgg
      721 ctcatccggt cgcggctccg tccttggtgc tcctcgtgct cgtcggcggt cacgccacgg
      781 cgcaggaagc tcgcgactgg ctcgagcgca tgagtcgcgc cgtcgaggac ttgaactacc
      841 gcggcacgtt cgtgcacgtg ctcgacggca ccgccgagac gctgcacatc gtgcatcgca
      901 acgccgacgg tcagagtggc gagcgcatca tttccatgga cggcgcaggt cgcgagatcg
      961 tgcgtcaagg cgatcgcgtt caaggcatct ttccggatcg gcgcatcgta tcgttcgaga
     1021 cgcgtaacga cggcagcgcg ctggtctccg cgctgcccag caccacgagc gatctcgagc
     1081 cgcactacga catgaagctc ggcggtgccg cgcgcgtcgc cgagcgcgga gtgcagatgc
     1141 tcgagatcaa accgcgcgac gagttccgtt acggctacat gctgtggctc gatcaagaga
     1201 cggctatgcc gctgcagtca cagctcatcg atgagcaggg gcaggtcgtc gagcagatcc
     1261 tgttcactga gatcgagatt ccggccgaga tcccagctgc agcgctcgag ccgacgatcg
     1321 acaccacggg cttcacgatg ctgcgggctg ccgaatcggc gccgctcgcg gcggaaattc
     1381 cctggcgcgc ggccgccgtg cccggcggct tcacgctctc ggtcgccacg cagagcccga
     1441 tggcgggctc cgacacgccg gtggagcacc tcgtctattc cgacgggctg gcaacggtct
     1501 cggtgttcat cgaggatccg aagactaaag ccgaggtcgg cgaaggtttc ttcagcgtcg
     1561 gcagcaccaa cgcgtactcg ttgacgctcg acggccgcaa ggtcacggcg atgggcgagg
     1621 ttccgcgtca gaccgtccgc aagatcgctt cgtcgctcgt cgcgcagtaa gcgagggctc
     1681 gagcatttcc ctttccggtt gcagcgccac cggcatcgtc cgagcgtcgg accgcgagca
     1741 catcgccgtc gagttcgccg cgccgccgcg ttgcaaaggt tgcggcggcg cgtgcctctg
     1801 gtatcgcgtg cccccgagcg aggagttgac gctggccgcg agcccggcga tccccgtcgg
     1861 cgccaccgtg gccgtgacac tgccggaccg ctatctgctg ctcggcgccg tgttggtcta
     1921 tggcgtgccg ctggcggcgc tcttggccgg cgccgggacg gccgccgccc tgttcggcac
     1981 cgatctcgcg gttgccgccg gtgcggtgct ggctctcgcg gccgccgtgg ccgccgcatc
     2041 gttcctgcgc gggcgcctcg agcgggcgac gctgcgccac ctcgccgtcc gtcgcgtttc
     2101 ctaacctcgg cgtgctcaag ctcacgctgt atagccggcc cgagtgccat ctttgcgagg
     2161 ctttcctggc ggacctcatg ccactgcttg gcgccgacat cgtcgtcgag acggtcgacg
     2221 tcgacagcag cgtcgcgctg gaacggcgct atgggctgcg aatcccggtg ctcgcggccg
     2281 gcgacgtcga gatctcgggc tacccgctcg accaggaccg cgttcgtcgc tatctcgagt
     2341 cgctctgagc tcgctcgagc gcagtgcttc cgcccaagcg gcggcgatac cgctataatc
     2401 cgcgccgccg ccaggggcgg ccacctaaca ccatggaacg gattcgtaac ttctcgatca
     2461 tcgcgcacgt cgatcacggc aagtccacgc tcgccgatcg gctcattcaa ctgtgcgggg
     2521 gtctcacggc gcgcgagatg gaagaccagg tgctcgactc gatggatctc gagcgcgagc
     2581 gcggcatcac gatcaaggcg cagagtgtcg cgctgcagta tcgcgcccgc gacgggctcg
     2641 actatcagat caacctcatc gacacgccgg ggcacgtcga cttctcgtac gaagtgtcgc
     2701 ggtcgctcgc cgcgtgcgac ggcgcgttgc tcgtcgtcga tgcgggccag ggcgtcgaag
     2761 cgcagagcgt agcgaactgc tacacggcca tcgagcaagg cctcgaggtg ctgcccgtca
     2821 tcaacaaaat cgatctgccg acggccgacc ccgagcgcgt catcgtccag atcgaggaca
     2881 tcatcggcat tgaagccaaa gacgccgtcc gcatcagcgc caagacgggg ttcggcgtcg
     2941 acgagctgct cgagcgcatc gtggagcgga tcccggcacc gaagggcagc gtcgacgcgc
     3001 cgctgcaagc gctgatcatc gactcgtggt tcgacagcta cgccggcgtc gtctcgctcg
     3061 ttcgcgtcat gaacggcgtc atccgcaagg gcgcgcgcat cgtcgtgcac tcgacgcagc
     3121 gcaagtatga ggtcgagcgg ctcggagtgt tcactccgcg tagcgcgccg cgcgaagagt
     3181 tgaaggccgg cgaggtcggc ttcctcattg cggcgatccg cgagat
//
LOCUS       DATLEE010000652         2619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3444919, whole genome shotgun sequence.
ACCESSION   DATLEE010000652 DATLEE010000000
VERSION     DATLEE010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3444919"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /locus_tag="VM692_14280"
     CDS             complement(<1..1858)
                     /locus_tag="VM692_14280"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53390.1"
                     /translation="MLTPAEQLGLAGAALDARVRQAVNFIPDSTLVHVARRLGEDARA
                     SDVVYTQGGTTETIRIMLRPLLVMPEQLGYLHHVCTRIMSALARFPELYARDPDVRRA
                     LPLADDERGWLDEVWPKVAHAEQPLYGRLDAVCDFTSARWQDSLKFMEPNLSGVGGMH
                     LGPLAETLVMRDVVPTLQNYDPLLAIDAPRDQRDLFLQVLLDHARTIGSRARNLALVE
                     PKYVADGPDEQSQLVEYFRSRRGIELVHADPRELRLVGDEVYYEKMRIDVAYRDYEIR
                     DLLALEHEHGTRLDAIRALFLQNRMVSGIGGDLDHKSCWEVLTSEELASRYFSVAERQ
                     LFRRHVLWTRVVRERRTDTPDGPMDLAEYIREYREELVLKPNRGYGGAGVQLGAALSQ
                     SEWETLLGTALAAQDDPHRQWVVQAAATLPVNLFPVLDEQGRTHEEPFYAVMGFAPTD
                     HGIGIIARVSQKQVVNVAQRGGLAAVLVGYRPEDLKSSIRSPIQSELAPQELRAVVQR
                     VRDLDAVIHLLEWDEETYRPRDAADDRASQLAVLGALRHDLLAGDRLGDLLAALAARR
                     DLQPREGLELERLGRLRRAAVALPQSLVAAFAETRSHCLAAWEEARRDEDDAA"
     gene            complement(1872..>2619)
                     /locus_tag="VM692_14285"
     CDS             complement(1872..>2619)
                     /locus_tag="VM692_14285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HUQ53391.1"
                     /translation="SSVTQPSFEEVARAAGERFALQLYTDGDEKWVVDTAKRAAQAGA
                     KALCLTVDVPTFGRRERHIHRRANMAGRAFGAMRTGEAHRGRADWTLVEKLKKAVKLP
                     LMLKGIQTAEDAELCLKYGVDIIYVSNHGGRQLDHGRGAIELLPEVISVARGKAEFII
                     DGGFVRGTDILKGVARGAAMVGVGKLQALSLGAAGERGVMRMLELLETELTVTMKLLG
                     LRAISELDGTYLQPATPVTVPGTLSALPLL"
BASE COUNT          393 a          925 c          891 g          410 t
ORIGIN      
        1 acgcggcatc gtcctcgtcg cgccgcgcct cctcccacgc cgcgagacaa tgcgagcgcg
       61 tctcggcaaa cgccgcgacg aggctctgcg gcaacgcgac ggcggcgcgg cgcaaccggc
      121 cgagccgctc gagctcgaga ccctcgcgcg gctgcaggtc gcggcgcgcc gcaagcgctg
      181 cgagcagatc gccgagccgg tcgccggcca ataggtcgtg gcgcagcgca ccgagcacgg
      241 cgagctgcga ggcacgatcg tcggccgcgt cacgaggccg gtacgtttcc tcgtcccact
      301 cgagcaggtg aatgacggca tcgagatcgc ggacgcgctg cacgacggcg cgcaactcct
      361 gcggcgcgag ctcactctgg atcggcgagc gtatcgagct cttcaagtcc tcgggtcggt
      421 agcccacgag cacggccgca aggccaccgc gctgcgcgac gttgacgact tgcttctgcg
      481 acacgcgcgc gatgatgccg atgccgtgat ccgtcggcgc gaaccccatg acggcgtaga
      541 acggctcctc gtgcgtgcgg ccttgctcgt cgagcacggg aaacaggttg acgggcaacg
      601 tcgcggccgc ctgcacgacc cattgccggt gcggatcgtc ctgcgcggcg agcgccgtgc
      661 cgagcagcgt ttcccattcg ctctgcgaca gcgcggcgcc gagttgcact ccggcgccgc
      721 cgtagccgcg attcggcttc agcacgagct cctcgcggta ctcgcgaatg tactcggcga
      781 ggtccatcgg cccgtccggc gtatccgtgc gccgctcgcg gacgacgcgc gtccatagca
      841 cgtggcggcg gaacagttgc cgctcggcga cgctgaaata gcgcgacgcg agctcctcgc
      901 tcgtcagcac ttcccagcag ctcttgtgat cgaggtcgcc gccgatgccg gagaccatgc
      961 gattctgcag gaacagcgcg cggatcgcgt cgagccgcgt gccgtgctcg tgctcgagcg
     1021 ccagcagatc gcgaatctcg tagtcgcgat acgcgacgtc gatgcgcatc ttctcgtagt
     1081 agacctcgtc gccgacgagc ctgagctcgc gcgggtcggc gtgcacgagc tcgatgccgc
     1141 gccgcgagcg gaagtactcg acgagctgcg actgctcgtc gggaccgtcg gcgacgtact
     1201 tcggctcgac gagcgcgaga ttcctcgcgc ggctgccgat cgtccgcgcg tggtcgagca
     1261 gcacttgcaa gaacagatcg cgctgatcgc gcggcgcgtc gatcgcgagc agcggatcgt
     1321 agttttgcag cgtcggcacg acgtcgcgca tgacgagcgt ttcggccaac gggccgagat
     1381 gcatgccgcc gacgcccgac aggttcggct ccatgaattt cagcgaatcc tgccagcgcg
     1441 cgctcgtgaa gtcgcagacg gcgtcgagcc tgccatacag cggttgctcg gcgtgcgcga
     1501 ccttcggcca cacctcgtcg agccagccgc gctcgtcgtc ggccagcggt agcgcgcggc
     1561 gcacgtcggg atcgcgcgcg tagagctccg gaaagcgcgc gagcgcgctc atgatccgcg
     1621 tgcagacgtg atgcagatag ccgagctgct cgggcattac gagcagcggc cgcagcatga
     1681 tgcggatcgt ctccgtcgtg ccgccttgcg tgtacacgac gtcgctggcg cgcgcgtctt
     1741 cgccgagccg ccgcgccacg tgcacgagcg tcgaatcggg aatgaagttc acggcctgcc
     1801 gcacgcgggc gtcgagcgcg gcgcccgcca agccgagttg ctccgccggc gtcagcacgg
     1861 cgccgcccgc gtcatagcag cggcaacgcg ctcagcgtcc cgggcaccgt gaccggcgtc
     1921 gcgggctgca gataagtgcc gtcgagctcg ctgatcgcgc ggaggcccag cagtttcatc
     1981 gtgaccgtca gctcggtctc gagcagctcg agcatgcgca tgacgccgcg ctcgccggcc
     2041 gcgccgagcg acagcgcctg aagcttgccg acgccgacca tcgcggcgcc gcgcgccacg
     2101 ccttttagaa tgtccgtgcc gcgcacaaag ccgccgtcga taatgaactc tgctttgccg
     2161 cgcgcgacgc tgatgacttc gggcagcagc tcgattgcgc cgcggccgtg atcgagctgc
     2221 cggcccccgt gattcgaaac gtagatgatg tcgacgccgt atttgaggca cagctcggcg
     2281 tcctcggccg tttggatgcc tttcagcatc agcggcagct tgacggcctt cttgagcttc
     2341 tcgacgagcg tccaatcggc gcggccgcga tgcgcctcgc cggtgcgcat cgcgccgaac
     2401 gcgcgacccg ccatgttcgc gcgccggtgg atgtgccgct cgcgccggcc gaaggtcggc
     2461 acgtcgacgg tcaagcacaa cgcctttgcg ccggcttgcg ccgcgcgctt cgcggtgtcg
     2521 acgacccact tctcgtcgcc gtcggtgtag agctgtagcg cgaagcgctc gcctgccgcg
     2581 cgcgcgactt cctcgaacga cggctgcgtg acgctgctg
//
LOCUS       DATLEE010000653         4182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10882711, whole genome shotgun sequence.
ACCESSION   DATLEE010000653 DATLEE010000000
VERSION     DATLEE010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10882711"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VM692_14290"
     CDS             complement(<1..824)
                     /locus_tag="VM692_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53392.1"
                     /translation="MATARLRFATVTLVCALVIAGCGSPPQSAPTASETDAAAKESAA
                     AAALRAAGAVDNLGATRWPSKAPAGASAPSFVVDASWPKPLPNDWRIGQVGGIAVDSH
                     DNIWVYHRPRTLDASSAGALPRVATNAQGVPISALGHARPYADRSTGCCVPAPSVLKF
                     DRAGNLLAAWGGPSDPGFLDEKCREAAGCFWPAREHGIFVDQNDFVWLSGNGEDGGAR
                     QQQNVATYPWAPTYGGDDSHILKFTADGTFVFQIGTAGMDGPNSEKIDGGPNGTPQP"
     gene            complement(893..1825)
                     /locus_tag="VM692_14295"
     CDS             complement(893..1825)
                     /locus_tag="VM692_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ53393.1"
                     /translation="MQPRARLTFAVRCSTVAALAALGLAQHAFAQQPAAPPADVKVTT
                     TLLGGNVYGIDGQGGRAAALIGPEGVFLVDAQFAAVSDKIAAAIKAITPSPIKLLVNT
                     HVHGDHTGGNENFAKLGATIIGRPNLREQLVNPRPAANGQTPAAAPAAALPVLLYDQP
                     LTIRLNGEEAQLIPLQPSHTNGDTAVKFVNADVLMTGDVFRSEGMPNAAAANGGNIVG
                     LLAVMEQFITLAGPNTKVVPGHGPVTNRAGLVAHRDMIVTVRDRVAKLKAEGKTLDEV
                     QAAKPTAEFDAKVGGQPQAITNFVAALYNAAPAR"
     gene            complement(1966..3336)
                     /locus_tag="VM692_14300"
     CDS             complement(1966..3336)
                     /locus_tag="VM692_14300"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUQ53394.1"
                     /translation="MSKSERGRIAVIGGGPAGSLLAILLARRGLESFVIERSPPFSST
                     AQTGGRSINLALAARGIAALRRAGIEADVAKLLIPMRGRMLHDVEGKQRFLPYGQRDT
                     EQIYSVSRAALNTLLYELAAQHLGVEYRFGEQCVGVERDGTPLIETRDGRRRHLAADV
                     VFAADGAGSEVRRALVANGAITASEELLDHGYKELTLAARDGAFALEANALHIWPRGG
                     FMLIGLPNVDRTFTATLFLPHRGEPSFASVGNDAEAFFAREFPDAVPLLPALARDYAS
                     HPVGHLGTVQCKPWSFANRVLLVGDAAHAIVPFHGQGMNAAFEDCVVLDELIGLHRRG
                     EGYDWPAIFAEFEAARAPNVRAIAEMALENYREMRDDVRDPKFQLRADLSFELERRFP
                     DRFIPRYSMVMFHPEISYAEAQRHGAQQARILRELTVTADSLREVDFALADDLVQASL
                     RPPTRR"
     gene            complement(3333..>4182)
                     /gene="kynU"
                     /locus_tag="VM692_14305"
     CDS             complement(3333..>4182)
                     /gene="kynU"
                     /locus_tag="VM692_14305"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HUQ53395.1"
                     /translation="QLRWHGREPRDALLEIGFRPGSFELDRERFEALLAEQGESIALV
                     LLPAVQYLTGERLDLGAYAAAAQRYGCRVGFDLAHAIGNVPLALHDSGADFAAWCSYK
                     YLNAGPGAVGGCFVHRRHGAAENLPRLAGWWGHDRERRFLMEPKFTPLSGAEGWQISN
                     PPIFSLAPLAASLALFDEAGLKRLRRKSLALTRYLEWLLANELGERARLLTPADPERR
                     GCQLAVQLVPPPADAVSFIARLRAAGVVADWRQPNVLRLAPVPLYNRFRDVYSAVQAL
                     RRTLDA"
BASE COUNT          633 a         1413 c         1486 g          650 t
ORIGIN      
        1 ggttgcggcg tcccgttcgg tccgccgtcg atcttctcgc tgttgggccc gtccatgcca
       61 gccgtgccga tctggaagac gaacgtgccg tcggccgtga acttcaagat gtgcgagtcg
      121 tcgccgccgt aggttggcgc ccacggatac gtcgcgacgt tctgttgttg acgcgcgccg
      181 ccgtcctcgc cgttgccgga caaccaaacg aagtcgttct ggtcgacgaa gatcccgtgc
      241 tcgcgcgcgg gccaaaaaca gccggcggct tcacggcact tctcgtcgag gaaacccgga
      301 tcggacggcc cgccccacgc cgcaagcaga ttccccgcgc ggtcgaactt cagcaccgaa
      361 ggcgcaggca cgcagcagcc cgtgctgcgg tcggcgtacg ggcgcgcgtg cccgagcgcg
      421 ctgatcggca caccctgcgc gttcgtcgcg acgcgcggca gcgcgccggc cgagctcgcg
      481 tcgagcgtgc gcggccggtg atagacccag atgttgtcgt gcgagtcgac ggcgatgccg
      541 cccacctggc caatgcgcca gtcgttcggc agcggcttcg gccagctcgc gtcgacgacg
      601 aagctaggcg ccgatgctcc cgcgggagct ttcgaaggcc agcgtgtcgc accgagattg
      661 tcgacggcgc cggctgcgcg caaggccgct gcggcggcac tctctttcgc cgcggcgtca
      721 gtctccgagg cggtcggtgc gctttgcgga gggctgccgc agcccgcgat gacgagcgcg
      781 cagacgagcg tgacggtggc gaatcgcaag cgtgctgttg ccatggctga cccccttcgg
      841 aaacgcgagc ggtcgacgcg gcgcgagccg cgccgaccgc gtgccgcggc gctcagcgcg
      901 ccggtgccgc gttgtacaga gccgcgacga agttcgtgat ggcttgcggc tgaccgccga
      961 ccttcgcgtc gaattcggcc gtcggcttcg cggcctggac ctcgtcgagc gtcttgcctt
     1021 ctgccttgag cttcgcgacg cggtcgcgca cagtgacgat catgtcgcga tgcgcgacga
     1081 gcccggcgcg gttcgtgacc gggccgtgac ccggcacgac cttcgtgttg ggccccgcga
     1141 gcgtgatgaa ctgctccatg acggccagca gcccgacgat gttgccgccg tttgcggccg
     1201 cagcgttcgg catgccttcc gagcggaaca cgtcgcccgt catcagcacg tcggcgttga
     1261 cgaacttcac ggccgtgtcg ccgttcgtgt gcgacggctg cagcgggatc agctgcgctt
     1321 cctcgccgtt caagcggatc gtcagcggtt gatcgtagag cagcacgggc aacgcggctg
     1381 ccggggccgc ggcgggcgtc tggccgttcg cggccgggcg cgggttcacg agctgctcgc
     1441 gcaggttcgg ccggccgatg atcgtggcgc caagcttcgc gaagttctcg ttgccgcccg
     1501 tgtgatcgcc gtgcacgtgg gtgttgacga gcagtttgat cggcgacggc gtgatcgctt
     1561 tgatcgcggc cgcaatcttg tccgagaccg cggcgaactg agcatcgacg aggaagacgc
     1621 cttcggggcc gatgagcgcg gcggcgcgtc cgccttggcc gtcgatgccg tagacgttgc
     1681 cgcccagtag cgtcgtcgtg accttgacgt cggccggcgg tgcggccggc tgctgcgcga
     1741 acgcgtgttg cgcaaggccg agagcggcca gcgcggcgac cgtggaacat cggacggcaa
     1801 aggtaagacg tgctcgtggc tgcatcgcga ttccccttaa gaagatggtg gtgggtattg
     1861 gacggggtag gcgatgacca ctataaggca gcgtcctccg attgttgagg ggccgtcgcc
     1921 gcctactcgc tgctgtgagg cgagttgtcg gtcttggagc gccgattagc gccgcgtcgg
     1981 cgggcgaagc gaggcttgaa ccaggtcgtc ggccagcgcg aaatccacct cgcgcaagga
     2041 gtcggccgtc accgtcaatt cgcggaggat tcgcgcctgc tgagcgccat ggcgctgggc
     2101 ttcggcatac gagatctcgg ggtggaacat caccatcgag tagcgcggga tgaagcgatc
     2161 gggaaaacgg cgctcgagct cgaacgacag gtcggcacgc agctggaatt tcggatcgcg
     2221 gacgtcgtcg cgcatttcgc ggtagttctc gagcgccatc tcggcgatcg cgcgcacgtt
     2281 cggcgcgcgc gcggcctcga actcggcaaa gatcgcgggc cagtcgtacc cttcgccgcg
     2341 ccgatgaagg ccgatgagct cgtcgagcac gacgcagtcc tcgaacgccg cgttcattcc
     2401 ttggccgtgg aacggcacga tggcgtgagc ggcgtcgccg acgagcagca cgcggttcgc
     2461 gaagctccac ggcttgcact gcacggtgcc caagtggccg acgggatggc tcgcgtagtc
     2521 tcgcgcgagc gccggcagca gcggcactgc atccggaaac tcgcgcgcga agaatgcctc
     2581 ggcgtcgttg cccacgctcg cgaagctcgg ctcgccgcgg tgcggcagga acagcgtcgc
     2641 cgtgaaagtg cggtccacgt tcggcagacc gatcagcatg aagccgccgc gcggccagat
     2701 gtgcagcgca ttcgcctcca gagcgaatgc gccgtcgcgc gctgccagcg tcagctcttt
     2761 gtagccatga tcgagcaact cttcgctggc cgtgatcgcg ccgttcgcga cgagcgcgcg
     2821 ccgcacttcg gagccggcgc catcggcagc gaacaccacg tcggccgcca ggtggcgcct
     2881 gcggccgtcg cgcgtttcga tcagcggcgt gccgtcgcgc tcgacgccga cgcactgctc
     2941 gccgaagcgg tattcgacgc cgaggtgctg tgccgcaagc tcgtagagca gagtgttcag
     3001 ggccgcgcgc gataccgagt agatctgttc ggtgtcgcgt tggccgtagg gcaagaaccg
     3061 ctgcttgcct tcgacgtcgt gcaacatgcg cccgcgcatc gggatcagca gcttcgcgac
     3121 gtcggcctcg atgccggcac gccgaagagc ggcgatgccg cgtgcggcca gcgcgagatt
     3181 gatcgagcgc ccgccggtct gtgcggtcga ggagaacggc gggctacgct cgatgacgaa
     3241 cgactcgagc ccgcggcgcg cgagcaagat cgcgagcaag ctgccggcgg ggccgccgcc
     3301 gatgaccgct attcgtcctc tctcggactt gctcatgcgt cgagcgtgcg ccgcagcgct
     3361 tgcacggccg agtagacgtc acgaaagcgg ttgtacagcg gcacgggcgc gagtcgcagc
     3421 acgttcggct ggcgccaatc ggccacgacg ccggccgcgc gcaggcgcgc gatgaacgac
     3481 accgcgtcgg cgggcggcgg cacgagctgc acggcgagct gacagccgcg ccgctcggga
     3541 tcggccggcg tcagcagccg tgcgcgctcg ccgagctcgt tcgcaagcag ccactcgagg
     3601 tagcgcgtca gcgccagcga cttgcgccgc aagcgcttca agccggcctc gtcgaacagg
     3661 gcgagcgagg ccgcgagcgg cgccagcgaa aaaatcggcg gattgctgat ctgccagccc
     3721 tcggcgccgg acagcggcgt gaacttcggc tccatgagga accggcgctc gcggtcgtgg
     3781 ccccaccagc ccgcgagccg cggcaggttc tcggcggcgc cgtggcgccg gtgcacgaaa
     3841 cagccgccga ccgctcccgg ccccgcgttc aagtacttgt agctgcacca cgcggcgaag
     3901 tcggcgcccg agtcgtgcag cgccagcggg acgttgccga tcgcgtgcgc gaggtcgaac
     3961 ccgacgcggc agccgtagcg ctgcgccgcc gcggcatacg cgccgagatc gaggcgctcg
     4021 cccgtcagat actgcaccgc cggcagcagc acgagcgcga tgctttcgcc ttgctctgcg
     4081 agcagcgcct cgaaacgttc gcgatcgagc tcgaagctgc ccgggcgaaa gccgatctcc
     4141 agcaacgcgt cgcgcggctc gcgcccgtgc cagcgcagct gc
//
LOCUS       DATLEE010000654         5608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10892916, whole genome shotgun sequence.
ACCESSION   DATLEE010000654 DATLEE010000000
VERSION     DATLEE010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10892916"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VM692_14310"
     CDS             <1..303
                     /locus_tag="VM692_14310"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HUQ53396.1"
                     /translation="SAYLAWCQRRRAAVDAAALCFASFVRQLKRLQVPARAPSEGPRA
                     YAERAAGSLPHAAARIRGVVDLYLRARYEPDSDGTALAALAAEVAAFRGERTSAAR"
     gene            complement(300..1886)
                     /locus_tag="VM692_14315"
     CDS             complement(300..1886)
                     /locus_tag="VM692_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HUQ53397.1"
                     /translation="MHEHLKRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGAVKGRK
                     ATRHATSDWMRLEQERGISITSSVMQFPYGDALINLLDTPGHEDFSEDTYRTLTAVDS
                     ALMVIDAAKGVEARTIKLMEVCRLRTTPIISFINKLDRDGRPPIELLDDIETVLDIEC
                     TPFTWPIGMGREFRGIYSLREDCAYLYQGRDGSRLPEVKAIHGLDSAELTAAVGETAA
                     KELREGVELVRGATPEFDHAAYLAGKQTPVFFGAAIHNYGIRELLDAFARLAPPPQPR
                     ATSARTVSPEEKTFSGFVFKIQANMDPAHRDRIAFLRICSGAYEPSMRMFHTRLGREV
                     RVGDAITFMAADRQHVEEAYAGDIIGLHNHGTINIGDSFTQGEPLTFTGIPSFAPELF
                     RRALLRDPLKLKALQKGLAQLCEEGATQLFRPQIGNDLILGAVGPLQFEVAAYRLRDE
                     YGVEAAFEGVGVVAARWIRSADPAQLDEFAKKLVANVARDHAGQLVYLAPSTVSLALT
                     QERWPKIQFFTTRESSAAAA"
     gene            complement(1906..2379)
                     /gene="rimI"
                     /locus_tag="VM692_14320"
     CDS             complement(1906..2379)
                     /gene="rimI"
                     /locus_tag="VM692_14320"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HUQ53398.1"
                     /translation="MSAAVHPRTEIRLMQPADLKAVAAVERAAYAYPWSLGIFRDCLL
                     AGYYCLVLDVGASVTGYGIMSIAAGEAHLLNLCVHPNAQRLGYGRRLLSSLMLKASDA
                     DADKIFLEVRPSNAVALRLYASVGFEQIGVRPAYYQAENGREDAVILAATLQPPR"
     gene            complement(2376..3107)
                     /locus_tag="VM692_14325"
     CDS             complement(2376..3107)
                     /locus_tag="VM692_14325"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HUQ53399.1"
                     /translation="MSAARDRYLEEIGIPVWRLRATASAATPLQAQEAPVVSGVAEAP
                     SFSAPATSALQPGADEWSELKAKVSACMLCGLHKSRTQTVFGVGRRDAQLFVIGEAPG
                     ADEDRQGEPFVGRAGQLLNAMLRAIGLPRSEVYIANILKCRPPGNRDPQPDESSSCTP
                     YLAQQIALVQPRVLMAVGRIAAQWLLQTDTPIGRLRGRVVPYGERNTPLVVTYHPAYL
                     LRSPLDKAKAWTDLCKVRELLSQPS"
     gene            complement(3104..3868)
                     /gene="pssA"
                     /locus_tag="VM692_14330"
     CDS             complement(3104..3868)
                     /gene="pssA"
                     /locus_tag="VM692_14330"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HUQ53400.1"
                     /translation="MDVRYPNTRRKGIYLLPNLVTTGGLFAGFYSIVASIDGNFHLAA
                     WAIFAAMLLDGLDGRVARLTSTASEFGKEFDSLADMVSFGLAPAIVVYQWGVERLAEY
                     GAVWGRLGWLAAFLYVAAAAFRLARFNSNVAQDRRFFQGLASPAAAAGVASMIWLATS
                     YDGFDGLAALVAGVTITAVTGLLMMSRFAYVSFKDVNPARRVRFVQLLLIPLVIIVIA
                     LEPPVTIFALFIVYAASGPAAWLWHRRGKRHDSVTP"
     gene            4059..4691
                     /locus_tag="VM692_14335"
     CDS             4059..4691
                     /locus_tag="VM692_14335"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006915073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HUQ53401.1"
                     /translation="MIHAVGSVLPTSVLSRLLPHVLMVLVAVGAATAEAQRRPSDARS
                     GPDPELRKILVAAIADSDSFADRFDAEVWLTDMSRRLARQVPEPQERLRILKTVHSQA
                     TRAGVAPELVLAVIDVESDFDRFAISSATALGLMQVMPFWVSELGYQDKNQLFDIEIN
                     VLLGCRILKYYLDMERGDLVRGLARYNGSVGKRWYSDRVIERLRTKWFQH"
     gene            complement(4695..4973)
                     /locus_tag="VM692_14340"
     CDS             complement(4695..4973)
                     /locus_tag="VM692_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HUQ53402.1"
                     /translation="MTQARAIRCVVAGRVQGVYYRAATAEQATRLQLDGWVKNLADGR
                     VEVVAAGSQPAIAELAGWLWQGPPAARVEAVHLEEWSGEVQRGFVVVK"
     gene            complement(4977..5264)
                     /locus_tag="VM692_14345"
     CDS             complement(4977..5264)
                     /locus_tag="VM692_14345"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HUQ53403.1"
                     /translation="MARALLMLVALVVGGCVSAPPVQEMSDARQAIAAAEQADAGRLA
                     ADTLADARRFLAEAEQQIQEQAYGPARMNALRAKNRAALALRSTRGRDNDN"
     gene            5384..>5608
                     /locus_tag="VM692_14350"
     CDS             5384..>5608
                     /locus_tag="VM692_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HUQ53404.1"
                     /translation="MKAKIYHNPRCSKSRATLALLTEHAVPYDVVDYLATPLSKTKLR
                     ELAHKLGAPALTLVRTEEPEFRDYVGRTLSD"
BASE COUNT          955 a         1902 c         1822 g          929 t
ORIGIN      
        1 agtgcctatt tggcctggtg ccagcggcga cgcgccgccg tcgatgccgc cgccctgtgc
       61 ttcgcgtcgt tcgtccgcca gctgaaacgt ctccaagtgc cggcgcgcgc gccgagcgaa
      121 gggccgcgcg cttacgccga acgagccgcc ggcagcctac cgcacgcggc ggcgcggatt
      181 cgcggcgtcg tcgatctgta cctgcgcgca cgctacgagc ccgattcgga cggcacggcg
      241 ttggccgctc tcgcagccga agtcgcggcg tttcgcggcg aacgcacttc ggcggcgcgt
      301 taagccgccg cggcgctcga ctcgcgcgtc gtgaagaact ggattttcgg ccagcgctcc
      361 tgcgtcagcg ccaggctcac cgtgctcggg gccaaataga cgagttgccc ggcatggtcg
      421 cgcgccacgt ttgcgacgag cttcttcgcg aactcgtcga gctgcgcggg gtcggcggaa
      481 cggatccacc gcgccgccac gacgccgacg ccctcgaacg ccgcctcgac gccgtactcg
      541 tcgcgcaagc gatacgccgc aacttcgaat tgcagcggtc cgacggcgcc gaggatcaag
      601 tcgttgccga tctgcgggcg gaataattgc gtcgcacctt cctcgcagag ctgcgcgaga
      661 cctttctgca gcgccttcaa cttcagggga tcgcgcagca acgcgcgccg gaacagctcc
      721 ggcgcgaagc tcggaattcc cgtgaacgtc agcggctcgc cttgcgtgaa gctgtcgccg
      781 atgttgatcg tgccgtggtt atgcaagccg atgatgtcgc cggcgtaggc ttcttcgacg
      841 tgttggcggt ccgctgccat gaacgtgatg gcgtcgccga cacggacttc gcggcccagg
      901 cgcgtgtgaa acatgcgcat gctgggctcg taggcgccgg agcagatgcg caagaacgcg
      961 atgcgatcgc gatgcgccgg atccatattc gcttggatct tgaacacgaa gccgctgaac
     1021 gtcttttcct cgggcgacac ggtgcgcgcg ctcgtagcgc gcggctgcgg cggcggcgcg
     1081 agccgcgcaa aagcgtcgag cagctcgcga atgccgtagt tgtggatcgc ggcgccgaag
     1141 aagaccggcg tttgcttgcc ggcgagataa gccgcgtgat cgaactccgg cgtggcgccg
     1201 cgcacgagct cgacgccctc gcgcagctcc ttcgcggccg tttcgccgac tgcggccgtg
     1261 agctccgcgc tgtcgagccc gtgaatcgct ttcacctcgg gcaatcggct gccgtcgcgc
     1321 ccctgataga gataggcaca gtcttcgcgc aaggagtaga taccgcgaaa ctcgcggccc
     1381 atgccgatcg gccacgtgaa cggcgtgcac tcgatgtcga gcacggtttc gatgtcgtcg
     1441 aggagctcga tcggcggccg gccgtcgcga tcgagcttgt tgatgaagct gatgatcggc
     1501 gtcgttcgga gccggcagac ttccatgagc ttgatcgtgc gcgcctcgac gcccttggcc
     1561 gcgtcgatga ccatcagtgc gctgtcgact gcggtcaacg tgcggtacgt gtcctcggaa
     1621 aagtcctcgt gacccggcgt gtcgagcaga ttgatcaacg cgtcgccgta cggaaactgc
     1681 atgacggacg acgtgatcga gatgccgcgc tcttgctcga ggcgcatcca gtcagacgta
     1741 gcatgccgcg tcgccttgcg gcccttgacc gcgcccgcca tctggatcgc gccgccgaac
     1801 agcagcagct tctccgtcag ggtggtcttg ccggcgtcgg ggtgcgaaat gatcgcgaac
     1861 gttcgccttt tcaggtgctc gtgcatgggg caacagtgaa ggacgtcagc ggggcggctg
     1921 cagcgtcgcg gccaggatga cggcatcctc gcgcccgttc tcggcctgat agtaggccgg
     1981 gcgaacgccg atctgctcga acccgactga cgcgtagagg cgcagcgcga cggcgttcga
     2041 cggccgcact tcgaggaaga tcttgtcggc atccgcgtcc gacgccttca gcatcagcga
     2101 gctcaacaga cgcctgccgt agcccaggcg ttgggcgttc gggtgcacgc agagattcag
     2161 caggtgcgcc tcgccggccg cgatcgacat gatgccgtag cccgtgacgc tggcgccgac
     2221 atccagcacc aagcagtagt aacccgccag caggcagtcg cgaaagatgc cgaggctcca
     2281 cggatacgcg tacgccgcgc gctcgacggc cgccaccgcc ttcaaatcgg cgggctgcat
     2341 gaggcgtatc tcggtacgcg gatggacggc ggcgctcatg acggttggct caacagttct
     2401 ctgaccttgc aaagatcggt ccacgccttg gctttgtcga gcgggctacg aagaagatac
     2461 gccgggtgat acgtcacgac cagcggcgtg ttgcgctcgc cgtaggggac gacgcggccc
     2521 cggagcctac cgatcggtgt atccgtctgc aataaccatt gtgccgcgat gcggccgacg
     2581 gccatcagca cgcgcggttg gacgagcgcg atctgctgcg caaggtacgg cgtgcacgac
     2641 gacgactcgt cgggttgcgg gtcgcggttg ccgggcggcc gacacttcaa gatgttggcg
     2701 atgtagacct cggagcgcgg caggccgatc gcacgcagca tcgcgttcaa cagctgccct
     2761 gcacgcccga cgaacggctc gccctgccga tcctcgtcgg cgccgggcgc ttcgccgatg
     2821 acgaacagct gcgcgtcgcg gcggccgacg ccaaacacgg tctgggtgcg actcttgtgc
     2881 aacccgcaga gcatgcaggc actgactttc gctttgagct cagaccattc atcggcgcct
     2941 ggctgcaacg cgctcgtcgc cggcgcgctg aagctcggcg cctcggcgac accggaaacg
     3001 accggcgctt cctgggcctg gagcggggtt gcagcgctcg ccgtcgcgcg gagccgccag
     3061 acggggatgc cgatctcttc gagatagcgg tcgcgcgccg cgctcacggc gtcaccgaat
     3121 cgtgccgttt gccgcggcgg tgccacagcc acgcggccgg ccccgaagcg gcatagacga
     3181 tgaacaatgc aaaaatcgtc acgggcggct cgagcgcgat gacgatgatc acgagcggaa
     3241 tcaacagcaa ctgaacgaac cgcacgcggc gtgccgggtt gacgtccttg aagctcacgt
     3301 aggcgaagcg cgacatcatc aggagaccgg tgaccgcggt gatcgtgacg ccggcgacga
     3361 gcgcggccag gccgtcgaac ccgtcatacg atgtcgcgag ccatatcatc gatgcgacgc
     3421 ccgcagccgc cgccgggctc gcgagccctt ggaagaaacg cctatcctgc gcgacgttgc
     3481 tgttgaagcg cgccaagcga aaggccgcgg ccgccacgta gaggaatgcc gcgagccaac
     3541 cgagccgccc ccaaaccgcg ccgtactcgg cgagacgctc cacaccccac tggtagacga
     3601 cgatcgcggg cgccaagccg aacgagacca tgtcggcgag gctgtcgaac tccttaccga
     3661 actcgctggc cgtgctcgtc aggcgagcga cgcggccgtc gagcccgtcg agcagcatcg
     3721 ccgcgaagat tgcccatgca gcgaggtgga agttgccgtc gatcgacgcc acgatggaat
     3781 agaagcccgc gaacaagccg cccgtcgtga cgaggttcgg cagtaagtag attcctttgc
     3841 gacgcgtatt ggggtaccgg acgtccacta acggttctcg agtgcccgga aagttcgggc
     3901 caatgtacca tccctgcccc cgtcggcaac cgctcctgcg ttgctctacc gcgggcatcc
     3961 ctgcccgcat cggcaaccgc tcctgcgttg ctctaccgct agcatccctg cccgcgtcgt
     4021 tgtgatccgc ttcgcgggca agcacaatcg ctgcgcgcat gattcacgcc gtgggtagtg
     4081 tgttgccaac cagcgtcttg tcgcgccttc tgccgcacgt gctcatggtg ttggtcgccg
     4141 tcggcgcggc caccgccgaa gcgcagcgcc gcccgagcga cgcccgctcg ggccctgacc
     4201 ccgagctgcg caagattctc gtggccgcaa tcgccgactc cgacagcttc gccgatcgat
     4261 tcgacgccga ggtatggctg acggacatgt cacgccggct cgcacgtcag gttccggaac
     4321 cgcaggagcg cctgcgcatc ctgaaaaccg ttcacagcca agcgacgcgc gccggcgtcg
     4381 cgcccgagct cgtgctcgcc gtcatcgacg tcgaaagcga tttcgatcgg ttcgcgatct
     4441 cgtcggcaac ggcgctcggc ctcatgcaag tcatgccgtt ctgggtttcg gagctcggct
     4501 accaggacaa gaaccagctg ttcgacatcg agatcaacgt gctgctcggc tgccgcatcc
     4561 tgaagtacta cctcgacatg gagcgtggcg acctcgtgcg cggcctcgcg cgctacaacg
     4621 gcagcgtcgg caagcgctgg tactccgacc gcgtcatcga acgcttgcgg acgaagtggt
     4681 tccagcattg agcgtcactt gacgacgacg aacccgcgct gcacctcgcc gctccactct
     4741 tcgagatgca cggcttcgac gcgcgctgcg ggtgggcctt gccagagcca gccggcgagc
     4801 tccgcgatgg ccggctgcga acccgcggcg acgacctcga cgcggccgtc ggccagattc
     4861 ttgacccagc cgtcgagctg caagcgcgtc gcctgctcgg ccgtcgcggc acggtaatag
     4921 acgccttgca ctcggcccgc gacgacgcag cgaatcgctc gagcttgcgt cacgctttag
     4981 ttgtcgttgt cacggccacg cgtcgagcgc aacgccagcg cagcgcggtt cttcgcgcgc
     5041 agggcgttca tgcgcgccgg accgtacgct tgctcttgga tctgttgctc ggcttccgcg
     5101 agaaagcggc gcgcgtccgc gagcgtatcg gctgcgaggc ggccggcatc ggcctgctcg
     5161 gcagcggcaa tcgcttgacg ggcatcgctc atctcttgaa ccggcggcgc gctaacgcag
     5221 ccaccgacca cgagcgcgac cagcattagc agtgcgcggg ccatcttgta attgttaggt
     5281 cgcaactgac gtacgccgcc ggaaaccgta cgcgccgcca cttacaccgt caaggcgccg
     5341 cgacggttct agtccgaccg ctcaaacctt aaacttcgag ccgatgaaag ccaaaattta
     5401 ccacaatccc cgttgcagca agtcgcgcgc caccttggcg ctgctgacag agcacgcagt
     5461 tccgtacgac gtcgtcgact atctggcgac gccgctgtcg aagacgaagc tacgcgagct
     5521 cgcgcacaag ctcggcgcgc cggcgctcac gctcgtacgc accgaagagc ccgagttccg
     5581 cgactacgtc ggacgcacgc tcagcgac
//
LOCUS       DATLEE010000655         3718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3462032, whole genome shotgun sequence.
ACCESSION   DATLEE010000655 DATLEE010000000
VERSION     DATLEE010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462032"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VM692_14355"
     CDS             complement(<1..840)
                     /locus_tag="VM692_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HUQ53405.1"
                     /translation="MRHSRRVVVSAGIGLAGAALVQRFESRAAEPRFSGDPFTLGIAS
                     GYPKPDAVVLWTRLAPEPLVAGGGMPAVAVDVEWELGADEQLRNVVRRGTVATTLDWA
                     HSVHVEVEGLSPARDYWYRFTSGGYQSPVGRTRTAAARGTTPQRLTLAVASCQHYEQS
                     YYAAYRGVVADSPDLVVHVGDYIYENRGTVRMRTHDQPECYTLDDYRLRYSLYKTDRN
                     LQAAHAAAPWLLVSDDHEVANDYAGEHSLQDDPRDVFLARRAAAYQAWYEHQPLPSQF
                     MPTA"
     gene            930..1838
                     /locus_tag="VM692_14360"
     CDS             930..1838
                     /locus_tag="VM692_14360"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HUQ53406.1"
                     /translation="MAVVATGDMGHAVGGALVRAGYRVVTAGEGRSAASRKLAADAGI
                     EDVRSLDAVVRSAHLVLSIVPPAAAQSFAATAARAMQAAGARPVFADCNAVAPSTVHS
                     IAETIAASGSPFADVGIVGRGPGPGGDRTRFYVSGAARAAVLELATSELELVDLGPDV
                     GTASAMKMAYSALNKGTDALLTAVLLAAERLGVRAPLLRELAGSQAEALKRMHARVPF
                     LGATAKRFAPEMAEIAKAYESVGVTPQFHRGAEWLYALVATTPYAAETRATQPKQRSL
                     DEALAVLTAALERDVRDPWKSPKGGA"
     gene            1835..2818
                     /locus_tag="VM692_14365"
     CDS             1835..2818
                     /locus_tag="VM692_14365"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HUQ53407.1"
                     /translation="MNDAVPYEHLTPDVIIDSVESCGFECTGSILGLASYENRVYQIG
                     VTDGFVVAKFYRPGRWSNEAILEEHDFALELAEQDIPVVAPLVRDGYSLREQEGYRFA
                     VYERRGGRWPELGTSEDREWLGRFLGRIHAVGATRRFKERPTLSREDMGARAAQQVLD
                     GDWLPPHLVASYERVTDQLVDAVERCFDEVGSYRQLRIHGDCHRNNVLWTDLGPHFVD
                     LDDCMNGPAVQDLWLFLAGARDEMAAQLGDILEGYGKFADFDYRELRLIEALRALRLI
                     HYTAWITRRWHDPAFPRAFPWLGEARYWEQHVQALLEQCAALDEEPLAVVV"
     gene            complement(2815..>3718)
                     /locus_tag="VM692_14370"
     CDS             complement(2815..>3718)
                     /locus_tag="VM692_14370"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HUQ53408.1"
                     /translation="QIRNVSPDSVHDARRAWANRSLTAWRDEARGMLRLTVEVPIEDG
                     ELILRALDCAVAGGEVTTDVDPDAIAESKSATWRAQQADALVAVMKSYLDGGQSPEGG
                     STADHYQVVVHTDAKAIAGGTGCADLPIDTLKRLLCDCSVVLVPEDGDGNPLHVGRKQ
                     RTVSTSLKRALYARDRGCTFPGCHRTRYLDGHHLRHWLNGGETNPDNMTLLCTHHHGL
                     LHWGAFSIVRNADGALRFVTADGRTIPRNGYRVEDFVDDDVGGETVDALRDAPRGGFC
                     TMAAESRPLDEVREPAAVYRLDAA"
BASE COUNT          559 a         1263 c         1300 g          596 t
ORIGIN      
        1 ggcggtcggc atgaactgac tcggaagcgg ctggtgctcg taccaggcct ggtacgcggc
       61 cgcgcgccgc gccagaaaca cgtcgcgcgg atcgtcctgc agcgagtgct cgccggcgta
      121 gtcgttggcg acctcgtgat cgtcggagac gagcagccat ggcgccgcgg catgcgccgc
      181 ttggaggttg cggtcggtct tgtagagcga gtagcgcaag cggtaatcgt cgagcgtgta
      241 gcactcgggt tggtcgtgcg tgcgcatgcg caccgtgccg cggttctcat agatgtaatc
      301 gccgacgtgc acgacgagat ccggcgagtc cgcgacgacg ccgcgatacg ccgcgtaata
      361 gctctgctcg tagtgctggc acgacgcgac ggcaagcgtg aggcgttgcg gcgtcgtgcc
      421 gcgcgccgcg gccgtgcgcg tgcggccgac ggggctctgg tagccgccgc tcgtgaagcg
      481 ataccaatag tcgcgtgcgg gcgagagtcc ttcgacttcg acgtggaccg agtgagccca
      541 gtcgagtgtc gtggcgacgg tgccgcggcg cacgacgttg cgcagctgct cgtcggcgcc
      601 aagctcccat tcgacgtcga cggctacagc cggcataccg ccgccagcca ccaacggctc
      661 gggcgcgagc cgcgtccaga gcacgacggc atccggcttg ggatagcccg acgcgatgcc
      721 gagcgtgaac ggatcgccgc tgaagcgcgg ttccgcggca cgagactcga agcgctgtac
      781 gagcgccgca ccggcgagcc cgatgccggc gctgacgacg acgcggcgcg aatgacgcat
      841 ctcgagcccc gctgacggct atgatcggaa gcggattgta cgctcgcaaa catgacgggt
      901 tcacggcgat gacggtaggg caacggacag tggcagtggt cgccacgggc gacatgggtc
      961 atgccgtggg tggcgcgctc gtgcgcgccg gttatcgcgt cgtgacggcc ggtgagggcc
     1021 gaagcgccgc gtctcgcaag ctcgcggccg acgccgggat cgaggacgtc cgatcgctcg
     1081 acgccgtcgt gcgcagcgcc cacctcgtgc tctcgatcgt gccgcctgcc gccgcgcaga
     1141 gcttcgccgc gacggcggcg cgcgcgatgc aagccgccgg cgcacggccc gtgttcgcgg
     1201 attgcaacgc cgtggcgccg tctacggtcc actcgattgc cgagacgatc gctgcgtccg
     1261 gctcgccgtt cgccgacgtc ggcatcgtcg gtcgcggccc cgggcccggc ggtgaccgca
     1321 cgcggttcta cgtttccggc gcggcacgcg ccgctgtgct cgagctcgcg acgtccgagc
     1381 tcgagctcgt cgatctcggc cccgacgtcg gcacggcgtc ggccatgaag atggcgtatt
     1441 cggccctgaa caagggcacc gacgcgctgc tgacggccgt gctgctcgcc gccgagcgcc
     1501 tcggcgtccg cgcgccgctg ctgcgcgagc tcgcgggcag ccaagccgag gcgctgaaac
     1561 gcatgcacgc gcgcgtgccg ttcttgggtg cgacggcgaa gcgcttcgcg cccgagatgg
     1621 cggagatcgc caaggcgtac gagagcgtcg gtgtcacgcc gcaattccat cgcggcgccg
     1681 agtggctcta cgcgctcgtc gcgacgacgc cgtacgcggc cgagactcgc gcgacgcaac
     1741 cgaagcagcg ctcgctcgac gaggcgctcg ccgtcttgac ggccgcgctc gagcgcgatg
     1801 tgcgcgaccc ttggaagtcc ccaaaggggg gcgcgtgaac gacgccgtcc cttacgagca
     1861 cctgacgccg gacgtcatca tcgactccgt cgaaagttgc ggcttcgaat gcacgggcag
     1921 catcctgggg ctcgcgagct atgagaaccg cgtctatcag atcggcgtca ccgacggctt
     1981 cgtcgtcgcg aaattctatc gcccggggcg gtggtcgaac gaagcgatcc tcgaggagca
     2041 tgatttcgcg ctcgagctcg cggaacagga catcccagtc gtggcgccgc tcgttcgtga
     2101 cgggtactcg ctgcgcgagc aagagggtta ccgcttcgcc gtgtacgaaa ggcgcggcgg
     2161 ccgctggccc gagctcggca cgagcgaaga tcgcgagtgg ctcggccggt tccttggaag
     2221 aatccatgcc gtcggcgcga cgcgccgctt caaggagcgt ccgacgttgt cgcgcgaaga
     2281 catgggcgcg cgcgcggcgc aacaggttct cgacggcgac tggctgccgc cgcatctcgt
     2341 cgcgagctac gagcgcgtta ccgaccagct cgtcgacgcc gtcgagcgct gcttcgatga
     2401 agtgggctcc taccgccagc tgcgcatcca cggcgactgc catcgcaaca atgtgctctg
     2461 gaccgacttg gggccgcact tcgtcgacct cgacgactgc atgaacggcc cggccgtgca
     2521 agatctctgg ctgttcctcg ccggcgcgcg cgacgagatg gccgcgcagc tcggcgacat
     2581 cctcgagggc tacggcaagt tcgccgactt cgattaccgc gagctgcgcc tcatcgaagc
     2641 gctacgcgcg ttgcgcttga ttcactatac ggcgtggatc acacgccgct ggcacgaccc
     2701 cgcgttcccg cgcgcatttc cgtggctcgg cgaggcgcgc tactgggagc agcacgtgca
     2761 ggcgctgctc gagcagtgcg cggcgctcga cgaggagccg ctcgccgtcg tcgtctaggc
     2821 ggcgtccaag cgatagaccg ccgcgggctc gcgcacctcg tcgagcggac gcgattccgc
     2881 tgccatggtg cagaatccgc cacgtggcgc gtcgcgcagc gcatcgactg tctcgccgcc
     2941 gacgtcgtcg tcgacaaagt cttcgactcg atagccgttg cgtggaatcg tgcgcccgtc
     3001 ggctgtgacg aaccgcagcg cgccgtcggc attgcgcacg atgctgaacg cgccccagtg
     3061 cagcagccca tggtgatgtg tgcacagcaa cgtcatgttg tcggggttcg tctccccacc
     3121 gttgagccag tgcctcaggt gatggccgtc gagataccgc gtgcgatggc aaccggggaa
     3181 cgtgcagccg cggtcgcgcg catagagagc gcgcttcagc gacgtcgata ccgtgcgttg
     3241 tttgcggccg acgtggagcg gattgccgtc cccgtcctcc gggaccagca ccacgctgca
     3301 atcgcagagc agccgcttca gcgtatcgat cggcagatcg gcgcagcccg tgcctccggc
     3361 gatggctttc gcgtccgtgt gcacgacgac ttggtagtga tctgccgtgc tgccgccctc
     3421 ggggctctgc ccgccgtcga ggtacgactt catgacggcg acgagcgcat ccgcttgctg
     3481 cgcgcgccac gtggcgcttt tggattcggc gatggcgtca ggatcgacgt ccgttgtcac
     3541 ctcgccgccg gcgacggcgc agtcgagtgc ccgcaggatc agctcgccgt cttcgatcgg
     3601 cacctcgacg gtgagccgca gcatcccgcg cgcttcgtcg cgccacgccg tcagcgaccg
     3661 gttcgcccag gcgcggcgag cgtcatgaac ggagtcgggc gagacgttgc gaatctgc
//
LOCUS       DATLEE010000656         3949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10109558, whole genome shotgun sequence.
ACCESSION   DATLEE010000656 DATLEE010000000
VERSION     DATLEE010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10109558"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1494)
                     /locus_tag="VM692_14375"
     CDS             complement(16..1494)
                     /locus_tag="VM692_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HUQ53409.1"
                     /translation="MGPSRRRRARSLARRLAAELAPAAVRRPPAETTKGRPPSETPSG
                     TSKGRPPGPRGWPYFGCLNGLLRNPMKFWSSIANRYGGIARVPLMKGHVAYLVSDPDL
                     LYELLITNRNKYRKNIRYRAAVELFGHGLLLNEGEAWKRQRLMSQPAFKADYIAAQVP
                     WMVQLTAKFLDGWRAFAADGRVHDVDADFLDLSQTLAGYYLMGPGFEPIAERFCRAAI
                     AVKDQWPQPPRSVRQLMRRRGDGWTEGLAAAVRDIDACITEYLVAERRRDFAGAGLVK
                     MLVDGSRAQNDEFDDRSLRDQILTVFFAGHETTATSLSWIHYLLDQHPEALRKLRTEV
                     AEVLGGRAPTSLDLERLVYTEQVINEALRLYSPIHSLSRVALEDDTIGGYHIPAGATI
                     YVSLYATHRLPSLWPQPDRFDPERFTPEQVERRPRFAFIPFAAGHRNCVGASTAIVEL
                     KLAVAMIAQRYELKLQPGHRVEGEAGTTMHPRYGMNMTIRAI"
     gene            1670..3742
                     /locus_tag="VM692_14380"
     CDS             1670..3742
                     /locus_tag="VM692_14380"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ53410.1"
                     /translation="MRSIGGCLALGLLALGLSVSSGARAQRNGAFTPKALTAPPRDGW
                     RTNGGNYYNQRYSPLAQIDRTNVAQLKGVWRTHLNGSGLAPQYSGEAQPIVHDGVIYI
                     STGANDVFALSVDTGAILWQYLAHIDAKITSVCCGWTNRGVGLGDGKVFFGRLDGRLV
                     ALDERTGKEVWDIQAEQWEEGFSLTSAPLYYDGMVITGFAGAEIGVRGRVKAYDADDG
                     KLLWTFYTIPGPGDAGHETWPQDNEVWMHGGATVWQTPAVDPELGLIYFSTGNPGPDF
                     NGAVRAGDNLFAASIVAVEARTGKYRWHFQEVHHDLWDYDAPNPVILFDVNIGGTVRK
                     GLAQVGKTGWAYILDRATGKPLVGIEERPVPQEPRQATAATQPYPLGDAIVPQQVDIA
                     PEGYELVNQGRIFTPFVGDKGMIVSPSLYGGANWPPSSYDPVRQRLFVCASDVTGNFI
                     GGDRDFEIPADGLHYEGGVVGFAPLPRTGIFAAVDVTTNKLAWRYRWADQCYSGSLAT
                     AGGLVFVGRSDGRVTALDSDTGLALWQFQTGAGMNAPTSSFERNGKQYVVGLSAGNVL
                     VGAPRGDSVWLFGLDGTLSPATQRDTPVTTAVPAPASAPAVAAAPAPASAEDGKRVFQ
                     QACVPCHGEDGMGGHGGGAPLDQVKDPELVVAMVNYGRGSMPPLQGALTNEQIRAVAA
                     YVTTELFK"
     gene            3752..>3949
                     /locus_tag="VM692_14385"
     CDS             3752..>3949
                     /locus_tag="VM692_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007105993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HUQ53411.1"
                     /translation="MIIDCHGHYTTAPKELGDYRDQQKAELKKNPQHQAQKGTLTIRD
                     DQLRASVENAQLKLQRERGTDV"
BASE COUNT          672 a         1308 c         1344 g          625 t
ORIGIN      
        1 ggctcgctca tgggtttaga ttgcccggat cgtcatgttc atgccatagc gcggatgcat
       61 cgtcgtgccg gcctcgcctt cgacgcgatg ccccggctgc aacttcagct cgtagcgctg
      121 cgcgatcatc gccacggcga gcttcagctc cacgatggcc gtgctcgcgc cgacgcagtt
      181 gcggtgaccg gccgcgaacg gaatgaacgc gaaccgtggc cgccgctcga cttgctcggg
      241 tgtgaagcgc tcgggatcga aacggtcagg ctggggccac agcgacggca ggcgatgcgt
      301 cgcatagagg gacacgtaga tcgtcgcgcc ggccgggatg tggtagccgc cgatcgtgtc
      361 gtcctcgagc gcgacgcgcg acaacgagtg aatcggcgag tagaggcgca gcgcctcatt
      421 gatgacttgc tccgtgtaga cgagccgctc gaggtccaag ctcgtcggcg cgcggccgcc
      481 gagcacctcg gcgacctcgg tgcgcagctt gcggagcgcc tcgggatgtt gatcgagcag
      541 gtagtgaatc cacgacagcg acgtcgccgt cgtctcgtgc cccgcgaaga acaccgttag
      601 gatctgatcg cgcagcgacc gatcgtcgaa ctcgtcgttc tgggcgcggc tgccgtcgac
      661 gagcatcttg acgaggccgg caccggcgaa gtcgcgtcgg cgctcagcga cgagatactc
      721 ggtgatgcag gcgtcgatgt cgcggaccgc cgcagcgagc ccttcggtcc agccgtcgcc
      781 gcgccgtcgc atgagctgcc gcacgctgcg cggcggctgc ggccattgat ccttcacggc
      841 gatcgccgcg cggcagaacc gctcggcgat gggctcgaag ccggggccca tcagatagta
      901 gcccgcgagc gtctgcgaaa ggtccaggaa atccgcgtcg acgtcatgca cgcgcccgtc
      961 cgcggcgaac gcgcgccagc cgtccaagaa cttcgccgtc agctgcacca tccagggcac
     1021 ttgcgcggcg atgtagtcgg ctttgaacgc cggctggctc atcaggcgtt ggcgtttcca
     1081 cgcttcgccc tcgtttagca acaagccgtg gccaaagagc tccacagcgg cgcggtaccg
     1141 aatgttcttg cggtacttgt tgcggttcgt gatcagcagc tcgtagagca ggtcgggatc
     1201 ggagacgaga taggcgacgt ggcctttcat caacggcacg cgtgcgatgc cgccgtagcg
     1261 gttcgcgatg ctgctccaga atttcatcgg gttgcgcagc aacccgttca agcatccgaa
     1321 gtatggccag ccacgcggcc cgggcggacg ccccttgcta gtccccgaag gagtctccga
     1381 agggggacgc cccttggtag tctccgcagg gggacgacgg acggcggccg gcgcgagctc
     1441 agcggccaag cgtcgtgcga gcgagcgagc gcgacggcgg cgcgatggcc ccaatcatca
     1501 tcagccaatt cccccgaacg aaagccgaac gcttcgtcgt tgtttttcga tcggctcctt
     1561 ttctagtcgg aaggcccggc cagtgtcaag cggccggaag gtatactgcg tcgcctcgag
     1621 gtgagtgagt cgacggatgg cggaacaggc ggtgcaacgg atgcgtgcgg tgcggtcgat
     1681 cggtggatgc ctggcgctcg gactgctcgc gctcggtctc agtgtatcga gcggcgcgcg
     1741 cgcgcaacgc aacggcgcgt tcacgccgaa ggcgttgacg gcgccgccgc gcgacggttg
     1801 gcgcacgaac ggcggcaact actacaacca acgctattcg ccgctcgcgc aaatcgaccg
     1861 gacaaacgtc gcgcagctca agggcgtttg gcgcacgcat ctcaacggtt ccggtctcgc
     1921 gccgcaatac tccggcgaag cgcagccgat cgtgcacgac ggcgtcatct atatcagcac
     1981 gggcgccaac gacgtgttcg cactgtccgt cgacaccggc gcgatccttt ggcagtacct
     2041 cgcgcacatc gacgccaaga tcacgtcggt ctgctgcggt tggacgaacc gcggcgtagg
     2101 cctcggcgac ggcaaggtgt tcttcggccg gctcgacggt cgcctcgtcg cgctcgacga
     2161 gcgcacgggc aaagaggtct gggacattca agccgagcag tgggaagagg ggttctcgct
     2221 gacgagcgcg ccgctctatt acgacggcat ggtcatcacg ggtttcgccg gcgccgagat
     2281 cggcgtgcgc ggccgcgtga aggcgtacga cgccgacgac ggcaagttgc tgtggacgtt
     2341 ctacacgatc ccgggtcccg gcgacgccgg ccacgagacc tggccgcaag acaacgaagt
     2401 gtggatgcac ggcggtgcga ccgtttggca gacgccggcc gtcgatcccg agctcggcct
     2461 gatttatttc tcgactggca accccggccc tgatttcaac ggcgccgtgc gggccggcga
     2521 caatttgttc gcggcgtcga tcgtggccgt cgaggcgcgc acgggcaagt accgctggca
     2581 cttccaagag gtccatcacg acctttggga ttacgacgcg ccgaatcccg tgattttgtt
     2641 cgacgtcaat atcggcggca ccgtgcgcaa ggggctcgcg caagtcggca agacgggttg
     2701 ggcctacatc ctcgaccgcg cgaccggcaa gccgctcgtc ggcatcgagg agcggcccgt
     2761 gccgcaggag ccgcggcaag cgacggcggc gacgcagccg tatccgctcg gcgacgcgat
     2821 cgtgccgcag caagtcgaca tcgcgccgga gggttatgag ctcgtgaatc agggccgcat
     2881 cttcacgccg ttcgtcggcg acaagggcat gatcgtgagc ccgagcctct acggcggcgc
     2941 gaactggccg ccgagctcct acgaccccgt gcggcagcgc ctcttcgtct gcgcgtcgga
     3001 cgtaaccggc aacttcatcg gcggcgaccg cgacttcgag attccggccg acggtttgca
     3061 ctacgagggc ggcgtcgtcg gcttcgcgcc attgccgcgg accggcatct tcgccgccgt
     3121 cgacgtcacg acgaacaagc tcgcctggcg ctatcgctgg gccgaccagt gctacagcgg
     3181 ctcgctcgcg acggccggtg ggctcgtgtt cgtcggccgc agcgacgggc gggtcacggc
     3241 gctcgactcc gacacgggcc tcgcgctctg gcagttccaa acgggcgccg gcatgaacgc
     3301 gccgacgagc tcgttcgaga ggaacggcaa gcagtacgtc gtcggcttat cggccggcaa
     3361 cgtgctcgtc ggcgcgccgc gcggcgacag cgtgtggcta ttcggtctcg acggcacgct
     3421 gtcgccggcc acgcagcgcg acacgccggt cacgacggcc gtgccggctc cggcgtccgc
     3481 gccggccgtc gcagcagccc cggctcccgc ctcggccgag gacggcaagc gcgtcttcca
     3541 gcaagcgtgt gtcccgtgcc acggcgaaga cggcatgggc ggtcatggcg gcggtgcgcc
     3601 gctcgaccag gtcaaagacc ccgagctcgt cgtcgcaatg gtcaactacg gccgcggctc
     3661 gatgccgccg ctgcaaggcg ctttgacgaa cgagcagatc cgcgccgtcg cggcctacgt
     3721 cactaccgag ctgttcaagt aacggacgcc catgatcatc gactgtcacg gccactacac
     3781 cacggcgccg aaagagctcg gcgactatcg cgaccagcag aaagcggagc tgaaaaagaa
     3841 cccgcagcat caagcgcaga agggcacgct gacgatccgt gacgatcagc tgcgggccag
     3901 cgtcgagaac gcgcagctga agctgcagcg cgagcgcggc acggacgtg
//
LOCUS       DATLEE010000657         2636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3471302, whole genome shotgun sequence.
ACCESSION   DATLEE010000657 DATLEE010000000
VERSION     DATLEE010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3471302"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..946)
                     /locus_tag="VM692_14390"
     CDS             complement(176..946)
                     /locus_tag="VM692_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HUQ53412.1"
                     /translation="MRPLSLVSLVLLLAAGPQAHAQDAAAATPPYVVPAAAPANVRRA
                     IASPDRTDEQRARDYHRKPAEILTASGIKDGDKVVEIAGFGQYFTTMLVAAVGPSGRV
                     DVYDLPYTERFAGPASREFDAKHANAVYHQESYNDVTLPQNVDSVWNVLYYHDLQPNG
                     VDTAAFNRKIFTALKPGGIFLVIDHKAEDGSGWRDAGTIHRMGVDTIKKEVLAAGFEL
                     ALESDLLANPGDDRSKMVFAPGTRGTTDQAFFIFRKPK"
     gene            1164..2420
                     /locus_tag="VM692_14395"
     CDS             1164..2420
                     /locus_tag="VM692_14395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUQ53413.1"
                     /translation="MSRRPAVVQSSRSSNTGFSEAGSIDAKTEREVERLRFAPETEAE
                     FQTYFVQRSLQRARWATAIYLALVAVVTAINMRGTMTPLSASVLQPIYLLRLGVACPA
                     LVLILAATVIPELQRHYQWIASTAVIVTGMSVMVISGLAAADGMPQFQMGDVLVVVYA
                     TLFLGLLFRAVMVVAGALLVSFVGIGWHLGVTSEHLIFASSVVFATTLMSVLSALRME
                     RLVRANFIENRLLNDIAERDGLSGLYNRRMFDNLTTRLWLQAQRNQESLQIILVDIDH
                     FKAYNDLYGHQAGDNCIRRVAAIIARAAKRPFDFCARYGGEEFVLVLYAPSVGSDPTA
                     LPEQIRRDTMAMNIPHTNSDAGVLTVSIGSATAEPGTKRSLAGLIQSADEALYRAKQL
                     GRNRAMHVDSADSSTPTGAFKVFAIK"
     gene            2506..>2636
                     /locus_tag="VM692_14400"
     CDS             2506..>2636
                     /locus_tag="VM692_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013464805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HUQ53414.1"
                     /translation="MNKVEETLEHWVFRSRWLLAPFFIGLLLAIIALLIKFVKQLAA"
BASE COUNT          441 a          868 c          845 g          482 t
ORIGIN      
        1 gcgcgcaaac tgcaatacgc gagcggattg cgccagagaa cctgcgtcac ctcgccttcg
       61 agctccacga cctgctgcgt atcgaagcgg ccgaagaagc tgtggtgggc ggccgccccg
      121 cccggcaacg cggcgccggc aagcaagagc aatccgacgc gcatcgcgct gccgttcact
      181 tcggtttgcg gaagatgaaa aacgcctggt ccgtcgtgcc gcgcgtacct ggagcgaaca
      241 ccatcttgct gcggtcgtca cctgggttcg cgagcagatc gctctcgagc gcgagctcga
      301 agccggctgc gagcacctct ttcttgatcg tgtcgacgcc catgcggtga atcgtgccgg
      361 cgtcgcgcca gccggagccg tcctcggcct tgtgatcgat gacgaggaag atgccgcccg
      421 gcttcagcgc agtgaaaatc ttgcgattga acgcagccgt atcgacgccg ttcggctgca
      481 ggtcatggta gtagaggacg ttccagaccg agtcgacgtt ctgcggcagc gtgacgtcgt
      541 tgtagctctc ctggtggtag acggcgttgg cgtgcttcgc gtcgaactcg cgcgacgccg
      601 gacccgcgaa tcgctccgta tacggcaagt cgtacacgtc gacacgtccg ctcgggccga
      661 cggcggccac gagcatcgtc gtgaagtact gcccgaaccc cgcgatttcg acgaccttgt
      721 cgccgtcttt gatgccggac gccgttagga tctcggccgg cttgcggtga tagtcgcgtg
      781 cgcgctgctc gtcggtccgg tccggcgacg cgatcgctcg gcgtacgttg gccggtgccg
      841 cggccggaac gacgtagggc ggcgttgcgg cggcagcatc ctgagcatga gcctgcggcc
      901 cggcggcgag cagcagaacg agcgaaacga gcgatagcgg tcgcatggcg gctccccttc
      961 gatcttcttc tggcgtgatg ccgagccatt ctgcgtgctt tcgcggtgcc agtgaactgc
     1021 cgccggcgcg ggcggcgcga ggccgggccc aaaacacgcg aaaagcgtgt atttgctggc
     1081 gttccaccgt ggctaagcgc gcgagttgcg gaccgttcca caattcgccc cggagccgtg
     1141 tgctaccgtg acgcgtgtcg caaatgtctc ggaggccagc cgtcgtgcag agctcgcgat
     1201 cgagcaacac cgggttcagc gaagccggct cgatcgatgc caagaccgag cgcgaggtcg
     1261 aacgcctgcg cttcgctccg gaaacggagg cggagtttca gacctacttc gtgcagcggt
     1321 cgctacagcg cgcccgctgg gcgaccgcga tctatctcgc gctcgtggcc gtcgtcacgg
     1381 cgatcaacat gcgcggcacg atgacgccgc tgtcggcgtc cgttctgcag ccgatctacc
     1441 tgctgcgtct cggcgtcgcg tgtccggcgc tcgtcctgat tctcgcggcc accgtcattc
     1501 ccgagctgca gcggcactat cagtggatcg ccagcacggc cgtcatcgtc acgggcatga
     1561 gcgtcatggt catcagcggg ctcgctgccg cagacggcat gccgcagttt cagatgggcg
     1621 acgtgctcgt cgtcgtgtat gcgacgttgt tcctcggctt gttgttccgc gccgtgatgg
     1681 tcgtcgccgg cgccctgctc gtgtcgttcg tcgggatcgg gtggcatctc ggagtcacga
     1741 gcgagcactt gatcttcgcg tcgtcggtcg tgttcgccac gacgctgatg tccgtcctgt
     1801 cggcgctgcg aatggagcgc ctcgtgcgcg ccaatttcat cgagaaccgc ctcctcaacg
     1861 acatcgcaga gcgcgacggc ctgagcggcc tttacaaccg acgcatgttc gacaacttga
     1921 cgacccggtt atggctgcag gcgcagcgca accaagaatc gctgcagatc atcctcgtcg
     1981 acatcgatca tttcaaagcc tacaacgacc tgtacggcca ccaggcaggc gacaattgca
     2041 tccgccgggt cgcggcgatc atcgcccgtg ccgcaaagcg gccgttcgat ttctgcgcac
     2101 ggtacggcgg cgaggaattc gttctcgtgc tctacgcgcc gtcggtcggc agcgacccga
     2161 ccgcgttgcc ggagcagatc cgccgcgaca cgatggcgat gaacattccg catacgaact
     2221 cggacgccgg agtgctgacc gtcagcatcg gctccgcgac ggcagagccg ggcaccaagc
     2281 gcagtctcgc cggcctcatc cagagcgccg acgaagcgct gtaccgcgcg aagcagcttg
     2341 gccgtaatcg cgccatgcac gtcgactcgg ccgactcgag cacgccgacc ggcgccttca
     2401 aagtcttcgc catcaaatag cgacgtctcg cctcgatgaa gtatagtcgc cgtcgcgcga
     2461 gcctcggtgc gtacccgcgc gcggccggtc acaacgagac agagcatgaa caaagtcgaa
     2521 gagactctgg agcactgggt atttcgcagc cgctggctgc tcgcgccgtt cttcatcggg
     2581 ctgctactcg ccatcatcgc gctgctcatc aaattcgtga aacagctggc ggcctt
//
LOCUS       DATLEE010000658         4177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7179483, whole genome shotgun sequence.
ACCESSION   DATLEE010000658 DATLEE010000000
VERSION     DATLEE010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7179483"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VM692_14405"
     CDS             complement(<1..784)
                     /locus_tag="VM692_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53415.1"
                     /translation="MSLRLQLLAVSLLTLVLPWMGLRYVQQMETALRAGLEQSLTSSA
                     AAVAAALAEQSATFCTPPDCDEGRTGATIYATALAREPELDGVRDDHWNTTPETALAI
                     GDGHQLWAGTYGRFAYLLLTVADRDLVYRSQPGQTPYGDRIVLATDPGVARWWLLTTA
                     APGAFRAQETAPDRFEPSDVYDARVIGVWRETAGGYTLEVRVPLNLIGEALGVGIIDV
                     DRSGADYVVAALDDNVTWDAATGAAGRFIRQRAELQSLLGQFA"
     gene            complement(797..1270)
                     /locus_tag="VM692_14410"
     CDS             complement(797..1270)
                     /locus_tag="VM692_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53416.1"
                     /translation="MRLVIVLLVALVGALAIGCASAQRGIVAPRITVQTLEPLPSSGG
                     QTRFRVRLLIDNQNTEPLKIRGVDFKLRLADQGILDGNSAVPLTIEALDRQTITLDLG
                     SEILSSLSRLMSFVQGPENALPYEIYGQVHLERRRLQPLQFSASGQVPLVMTGQR"
     gene            complement(1272..1718)
                     /locus_tag="VM692_14415"
     CDS             complement(1272..1718)
                     /locus_tag="VM692_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53417.1"
                     /translation="MKMLSTAAAIAAAVVAGCGTSDPEAEIHARLADAEAAAEARDVG
                     FFGDLVGEGYRDSHGHDRAELLRVLRGLFLANQRVEIVSRIDEVELEGGDAARVVLHA
                     GMLGQRSGEAALAGIDADLYRFELELVNDGGEWQIIGAEWTRGLGD"
     gene            1827..3110
                     /locus_tag="VM692_14420"
     CDS             1827..3110
                     /locus_tag="VM692_14420"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HUQ53418.1"
                     /translation="MRNFEARRVAVIGGVRIPFCRAHTAYARCSNQDMMTAVLQALVK
                     RYALTGERLGDVSLGAVIKHSRDFNLARESTLSSGLAPETPAFDIQRACGTSLSASII
                     IGSKISSGLIEAGIAGGTDSISDVPLVYPDAYRALLLESARGRSFGARLSPWLKLRPK
                     HFKPIFPDVMEPRTKLSMGESMEITAKEWSLSREEQDALALASHQNATRAYAEGFYDD
                     LVVEFMGVKRDNNIRADTSLEQLAKLKPVFDRGPRGTLTAGNSTPLTDGASAVLLSSE
                     EWAQSHGLRAAAYLTHAREAAVEFVAKEGLLMAPAYAVSAMLAEANLTLQDFDFYEIH
                     EAFAAQTLVTLKAWESEKFCRERLGRDRAMGSIDRSKLNIKGGSVAVGHPFAATGTRI
                     VATLTKLLQQRGSGRGLISVCTAGGMGVTAILEAA"
     gene            complement(3111..4001)
                     /locus_tag="VM692_14425"
     CDS             complement(3111..4001)
                     /locus_tag="VM692_14425"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HUQ53419.1"
                     /translation="MIAEVAAVLAPVFVLAAIGYGWRLTGAPFDMPFVTRIIMYVAGP
                     CLVFTSLSQLTLPLSQFWGMVGAMIVVTLVVAVVSAVLLRILKLPLRSFVPALTLGNT
                     GNLGLPLCLFAFGEEGLGLAVAIYVTNSLVQFTFVPLLHTRVSLLRTAFGTPMLWGAV
                     AGLTALLADVQLPQWLARTVESIGDLLIPLMLMALGNTVGGLKANNLPRALGLGAARL
                     IISFVAVVSVSFALGLSGVAQGVLVLQGAMPAAVFSYLFAARYERDADDVAGIVLVST
                     LLGAVTLPLLVSYALWLARS"
BASE COUNT          758 a         1421 c         1364 g          634 t
ORIGIN      
        1 gagcgaactg gccgagcagg ctttgcagct cggcgcgctg ccggatgaac cgcccggccg
       61 cgccggtcgc tgcgtcccac gtcacgttgt cgtcgagcgc cgcgacgacg tagtcagcgc
      121 cgctgcgatc gacgtcgatg atgcctacgc cgagcgcctc gccgatcaga tttagcggca
      181 cgcgcacctc gagcgtgtag ccgccggccg tctcgcgcca gacgccgatc acgcgtgcgt
      241 cgtagacgtc gctcggctcg aagcgatccg gcgcggtttc ctgcgcgcgg aatgcgccgg
      301 gcgccgccgt cgtcaacagc caccagcgcg cgacgccggg atcggtcgcg agcacgatgc
      361 gatcaccgta aggcgtctgg cccggctggc tgcgatagac gaggtcgcga tcggcgaccg
      421 tcagcaacag atatgcgaat cgaccgtagg tacccgccca gagctgatga ccgtcgccga
      481 tcgcgagcgc ggtctcgggc gtcgtgttcc aatgatcgtc gcgcacgcca tcgagctccg
      541 gctcgcgcgc gagcgccgtc gcgtaaatcg tcgcgccggt gcgcccctcg tcgcagtcgg
      601 gcggcgtgca gaacgtcgcg ctctgctctg ccagcgcggc cgccacggcc gcggcgctcg
      661 acgtcagcga ctgctcgagg ccggcccgca gagccgtctc catttgctgc acatagcgca
      721 accccatcca tggcagcacg agcgtcagca agctgacggc gagcaactgc aggcggagcg
      781 acaagggcgt tggccgtcag cgctggcccg tcatcacgag cggcacttgc ccgctcgcgc
      841 tgaactgcag cggttgcagg cggcggcgct cgagatgcac ctgtccgtag atttcatacg
      901 gcagcgcgtt ctcgggccct tgcacgaacg acatgagccg cgacagcgac gacaggatct
      961 cgctgccgag gtcgagcgtt atcgtctgtc gatcgagcgc ctcgatcgtc agcggcacgg
     1021 ccgagttgcc gtcgaggatg ccttggtcgg ccaagcgcag cttgaagtcg acgccacgga
     1081 tcttcagcgg ctcggtattc tgattgtcga tcagtaagcg gactcgaaac cgcgtctgtc
     1141 cgccgctcga cgggagcggc tccagcgtct gcaccgtgat gcgcggcgcg acgatgccgc
     1201 gctgcgcgga cgcgcaaccg atcgcgaggg cgccgaccaa tgccaccaac aacacgatta
     1261 cgagccgcat ctcaatctcc caagcctcga gtccattcgg cgccgatgat ctgccactcg
     1321 ccgccgtcgt tcacgagctc cagctcgaaa cgatagaggt ctgcgtcgat gccggccaga
     1381 gctgcctcgc cggaacgttg gccgagcatg ccggcgtgga gcacgactcg cgcggcatcg
     1441 ccgccttcga gctcgacttc gtcgatgcgg ctgacgatct cgacgcgttg gttcgcgaga
     1501 aacaacccgc gcagcacgcg cagcagctcg gcgcgatcgt gcccgtgcga gtcgcggtag
     1561 ccctcgccga cgaggtcgcc gaagaacccc acgtcacgcg cttctgcggc agcttcggca
     1621 tcggcgagcc gcgcatggat ctcggcctcg ggatcgctgg tgccgcagcc ggcgactacg
     1681 gccgcggcaa tcgcggcagc cgtggagagc attttcatgc ggcgattgta gagtgcggcc
     1741 cggcccaaaa acacgacgcg gtaccgcacc acggcacttc cacgcaccct tatactggtc
     1801 cactagcgtt tgagcggagc gtggcaatgc gcaatttcga ggctcgtcgg gtcgccgtga
     1861 tcggcggcgt tcgcattcct ttttgccgtg cgcacacggc ctacgcgcgc tgctcgaatc
     1921 aagacatgat gaccgccgtg ctgcaagcgc tcgtcaagcg ctacgcgctg accggcgagc
     1981 gcttgggcga cgtctcgttg ggcgccgtca tcaagcattc gcgcgacttc aatctggcac
     2041 gcgaatcgac gctatcgtcg ggtctcgcgc cagagacgcc ggcgttcgac atccagcggg
     2101 cctgcggaac gagcttgtcg gcatcgatca tcatcggcag caagatctcg agcgggctca
     2161 tcgaggccgg catcgctggc gggaccgatt cgatcagcga cgtgccgctc gtctatcccg
     2221 acgcatatcg cgcgctactg ctcgagagcg cgcgcgggcg ctcgttcggc gcccgcctgt
     2281 cgccctggct caagttgcgg ccgaagcact tcaagccgat ctttcccgat gtaatggagc
     2341 cccgcacgaa gctctcgatg ggcgagagca tggagatcac ggccaaggag tggagcttgt
     2401 cgcgcgaaga gcaggatgct cttgccctcg caagccatca gaacgcgacg cgcgcgtatg
     2461 ccgagggttt ctacgacgac ctagtcgtcg agttcatggg cgtcaagcgc gacaacaaca
     2521 tccgcgccga tacgagcctc gagcagctcg cgaagctgaa acccgtgttc gaccgcggcc
     2581 cacgcggaac gctgacggcc ggcaacagca cgccactgac cgacggcgca tccgccgtgc
     2641 tgctgtcatc ggaggaatgg gcgcagagcc atggattgcg cgcggccgcg tacctgacgc
     2701 atgcccgcga agccgccgtc gaattcgtcg ccaaggaagg gctgctgatg gcgcccgcgt
     2761 acgccgtgtc ggcgatgctg gcagaggcca atttgacgct gcaggacttc gacttctacg
     2821 aaattcacga agcgtttgcc gcgcaaacgc tcgtgacctt gaaagcctgg gagtcggaga
     2881 aattttgccg cgagcggctc gggcgcgatc gtgccatggg gtcgatcgat cgcagcaagc
     2941 tcaacatcaa aggcggcagc gtggccgtcg gccatccgtt cgccgcgacc ggcacgcgta
     3001 tcgtcgcgac gcttacgaag ctactacagc agcgcggcag cggccgcggc ttgatctccg
     3061 tctgcacggc cggcggcatg ggcgttaccg cgatcctcga agccgcctga tcaggatcgc
     3121 gcgagccaca gcgcgtacga cacgagcaac ggcaacgtga cggcgccgag caatgtcgac
     3181 acgagcacga tgcccgcgac gtcgtcggca tcgcgctcgt agcgcgccgc gaacaggtag
     3241 ctgaacacgg cggccggcat ggcgccttgc agcacgagca ctccttgcgc gacgccggac
     3301 aggccgagcg caaagctgac gctgaccacg gcgacgaacg aaatgatcag ccgcgccgct
     3361 cccaagccga gcgcgcgcgg caagttattc gccttcaagc cgccgaccgt gttgccgagc
     3421 gccatcagca tcagcggaat cagcaggtcg ccgatcgact cgacggtcct tgcgagccat
     3481 tgcggcagct gcacgtcggc gagcagcgcg gtgagccccg cgaccgcgcc ccacaacatc
     3541 ggcgtcccaa acgccgtccg caacagcgag acgcgcgtgt gcagcagcgg cacgaacgtg
     3601 aactgcacga gcgagttcgt gacgtaaatc gcgacggcaa ggccgagccc ctcttcgccg
     3661 aacgcgaaca agcacagcgg caagcccagg ttgcccgtgt tgccgagcgt caacgccggg
     3721 acgaacgacc gcagcggcag cttgaggatc ctcagcagca cggcggacac aacggcgacg
     3781 accagcgtga cgacgatcat cgcgccgacc atcccccaga actgcgagag cggcagcgtc
     3841 agctgcgaca gactcgtgaa cacgaggcac gggccggcga cgtacatgat gatgcgcgtc
     3901 acgaacggca tgtcgaacgg cgcgccagtg aggcgccagc cgtagccgat cgccgccaag
     3961 acgaacaccg gcgcgaggac ggcggcaact tcggcgatca cgaacctggg tctcggttat
     4021 tcggctggat agcggtcggc gaggcgcggc gaagtctgcc agaatggcgc gcccaagtct
     4081 caggcccgaa ctcaaggcaa gatgctcgga cagttcttgg agtactcgat cgcggcgcag
     4141 ccgctcgcac cctcgttcga gttctttcgc tcgctcg
//
LOCUS       DATLEE010000659         7446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8691064, whole genome shotgun sequence.
ACCESSION   DATLEE010000659 DATLEE010000000
VERSION     DATLEE010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8691064"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VM692_14430"
     CDS             <1..458
                     /locus_tag="VM692_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HUQ53420.1"
                     /translation="QRNGIEAAGLSGDVPQRKRLRMLKDFQAGKLAVLIGTDVASRGL
                     HVPGVSHVFNYDLPQDPEDYVHRIGRTARAGATGDAISFGCETYAISLPDIEAFIGRK
                     IPVASFNHDALMTGKPNAPRTHRPHHGGPGGGQRRGPPRRRRQGDGPRR"
     gene            complement(466..1149)
                     /gene="yrfG"
                     /locus_tag="VM692_14435"
     CDS             complement(466..1149)
                     /gene="yrfG"
                     /locus_tag="VM692_14435"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010321547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HUQ53421.1"
                     /translation="MTGAAKSIVAHPIVDWDGIDTVLVDMDGTLLDLNFDNRFWREIV
                     PQRYARLRGMTVSDAQESLAPRFEAKIGTLEWYCLDHWARDLGMDLKSLKHEHREHIR
                     FLPGAPKFLASVRARGKRLSIVTNAHRDTFAVKAEQTGIDRLVDTVVCSHDFAAPKES
                     HAFWRALENHAPFDVRRTLLIEDSLAVLAAARAYGFERTIAIRRPDSRQPPRVIAEFT
                     AVDGVHELV"
     gene            1173..2003
                     /gene="cysQ"
                     /locus_tag="VM692_14440"
     CDS             1173..2003
                     /gene="cysQ"
                     /locus_tag="VM692_14440"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HUQ53422.1"
                     /translation="MTDLNRRLLDAACEIARDAGRAILEVYGRDDFAVARKSDDSPLT
                     EADQVAHGIITGALAKLDSALPILSEESRPAEHATRRSWPRYWLVDPLDGTKECLKRN
                     GEFTVNIALVDGQRAVLGAVHAPVLDRMYSGVVGLGAWRSDAGAAPRPIAVRGTAATP
                     LRVVGSRSHPSPELAAYLAGLPAHEITDMGSSLKICLVAEGAADVYPRLGPTSEWDTA
                     AAQAILESAGGRMIDLAGRPLRYNSKDDLLNPHFLAFGDHRRDWLAPIQNTRGLQSRN
                     "
     gene            2017..2196
                     /locus_tag="VM692_14445"
     CDS             2017..2196
                     /locus_tag="VM692_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HUQ53423.1"
                     /translation="MERLKQLRVEHRDLDQVIARLVTDPNVDQLMLKRLKKRKLMLKD
                     MITQLESARIPDLNA"
     gene            2216..3151
                     /gene="glk"
                     /locus_tag="VM692_14450"
     CDS             2216..3151
                     /gene="glk"
                     /locus_tag="VM692_14450"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HUQ53424.1"
                     /translation="MADIGATMTRCALVGEKGQELAPETFENADFTGVEGVLRVYLDH
                     RRASDRPSRAALAVAAPIVGDEVQMINIGWRFSQSQLQQALDLKRLQIVNDFAANAWA
                     LPQLEPADIAPIGGGESAPRTTLAVLGPGSGLGVAGLVPTGDGWAVMTGEGGHVSMPA
                     ATREEQDVIALLRDRFDGHCSAERVLSGPGLVNLYVALAELAGRGHPTVNPEDVTNLA
                     RQGEPLARKTLAMFFAMLGTVAANLALTAGARGGIYIAGGIVPRFVEQLRKSEFRARF
                     ENKGRYRSYLAAIPTHVITAPLPAFRGLKYLLGYR"
     gene            complement(3185..3415)
                     /locus_tag="VM692_14455"
     CDS             complement(3185..3415)
                     /locus_tag="VM692_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53425.1"
                     /translation="MRLGAGLWCATALALTLAACGSSEPEERASDSREAAPRRETVFD
                     PLTSTIGRAQSAQQTVDQQAAEQRRRIEEAER"
     gene            3558..5084
                     /locus_tag="VM692_14460"
     CDS             3558..5084
                     /locus_tag="VM692_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ53426.1"
                     /translation="MTSATAPAKASLDPIKVLCWVGIAAYVVVKFLRWQQGATLGELF
                     DFGDPSLWGLMFGACCVGLILRSPKIRRSATLIEANETRAVTWSFLTGFTVMSAYFFL
                     RPVRDSMASDWSDAEISHLWTIQFFLSLGLVMVYGLACSKIRFRYLVPAVYTFYAVTF
                     ALFYLGSKAMTNAVLLDKSFYVWVSLFSLFHLSVFWSFMADTFSSEQSKRLFPFIGVG
                     VSLGAAAAPLVVAVIASDVGNQNLMLVAAVILLIAIPMIFYLQRLKSADLHNEDVRAN
                     LGAATMGGKWWQGFRDFATNPYLLTIGVFILLYTAMQAFIYFETTELLRIYEAREERT
                     TILALRDAVANILTFGLGLFVTSRLVKRLGMPWTLALLPAFCALGFLILATAPVLSVL
                     LAVDIARRGGEYGITRPAREILFTSVDRETRFKTKPVIDVVVYRGGDAWWGGIFAAMS
                     EGIGLGFAAMSLIGSAFAAVWIAASLYLGRAFERRERDVESTAPAPTPPREAVAGHRG
                     "
     gene            complement(5081..>7446)
                     /locus_tag="VM692_14465"
     CDS             complement(5081..>7446)
                     /locus_tag="VM692_14465"
                     /inference="COORDINATES: protein motif:HMM:NF042738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6701 domain-containing protein"
                     /protein_id="HUQ53427.1"
                     /translation="QSGAQRMYINGVLQGATGNFTGTLATNTVPLYIGTDWNFISRAF
                     DGYIDEVHIVADALTQAEIQALRDETHPCANTARFTITHNAFGINCAPETITVNVIDS
                     ITGTPLVNYNAAVQLDTQSSNGTWALVTGSGAFSDGTAGDGAATYTWPLGQSQATFTL
                     YYPQGPPSLDVDVFQISNTGVRDNDAEGALVFSPNGFTVTAAALANPPGAVTQFAANQ
                     TAGTNFTVHLAAYGQTPTDSVCGIIEAYTGAKNLKFWSQYVDPGTGTRNVTINGASAA
                     ASEPASVAQAVTFTNGQASVVAKYKDVGRIRVLMKDDSTVNAELPSGITGATANFVVR
                     PYDFLLSGIANAANTVVNPQAPDATGAVFLAAGAPFRATVTARDAEGSTTPNFGRESV
                     PETVRLTTQLVAPAGGSSPPIGYTTGFGSFTNGVATGTDFTWSEVGIVRAVPGIGDAN
                     YLTAGDVTGTASERIGRFIPSRFVAVLNSPLFATACSSFTYQGQTFGYTTPPVITATA
                     VGVSGSTTTNYTGSFFKLSNTTLTGRSYSSVAGALNVAGLPATAVDPAIASPSGGVAT
                     LTFSSGSGIAFVKGAAQAPFNAQVQLAINVLDADGVAAVGAAPLGNPVTFGAAGGIPF
                     TAGQEIRYGRVRVGTAVGSELVDLPVPVRAEYYASAAAGFVTNAADACTTNVSLSFPA
                     YAENLNAGETCVRDSGSPGASALGCAAAAAAPIRYREPPLAGDFNLNLAAPGAGNQGS
                     VLVRAAVPAWLRFDWDAGAAGDEDPTGQATFGIFGGDRRTIYTREIY"
BASE COUNT         1181 a         2452 c         2500 g         1313 t
ORIGIN      
        1 tccagcgtaa cggcatcgag gccgcagggt tgtcgggcga cgtgccgcag cgcaagcggc
       61 tgcggatgct gaaggacttt caggccggca agctcgccgt gctgatcggc accgacgtcg
      121 cgtcgcgcgg cctgcacgtg ccgggggtta gccacgtgtt caactacgat ctgccgcagg
      181 atcccgagga ctacgtgcat cgcatcggcc gcacggcgcg cgccggcgca acgggcgacg
      241 cgatcagctt cggctgcgag acctacgcga tctcactgcc cgacatcgag gcgttcattg
      301 gccgcaagat tcccgtcgcg tcgttcaatc acgatgcgct gatgaccggg aagccgaacg
      361 cgccgcggac gcatcggccg catcacggcg gccccggcgg agggcaacgc cgcggcccac
      421 cgcgccgcag gcggcaaggg gacggcccgc gtcgctgacg tttgcctaga cgagctcgtg
      481 caccccgtcg acggcagtga attcggcgat gacgcgtgga ggctgacgcg agtcggggcg
      541 ccgaatggca atcgtgcgct cgaaaccgta ggcgcgcgcg gccgccagca cggcgaggct
      601 atcctcgatc aacaacgtgc gccgcacgtc gaacggcgcg tgattctcga gggcacgcca
      661 aaaggcatgg ctctctttgg gtgccgcgaa atcgtgtgag cagacgacgg tgtcgacgag
      721 ccgatcgatc ccggtttgct cggctttgac ggcgaacgtg tcgcgatgcg cgttcgtgac
      781 gatcgacaga cgcttgccgc gagcgcggac gcttgccaaa aatttcggcg cgcccggcaa
      841 gaaccggatg tgctcgcgat gctcgtgctt cagcgacttc agatccatgc cgaggtcgcg
      901 ggcccaatga tcgaggcaat accattcaag cgtgccgatc ttggcctcga agcgcggcgc
      961 gagcgactct tgcgcgtccg acaccgtcat gccgcgcagg cgcgcgtaac gttgcggcac
     1021 gatctcgcgc cagaaccgat tgtcgaagtt gagatcgagc agcgtgccat ccatgtcgac
     1081 gagcacggta tcgatgccgt cccaatcgac gatcggatgg gcgacgatcg acttggctgc
     1141 tcctgtcatg tcgctatgat acaagacgac gtatgacgga tttgaaccga cgcctgctcg
     1201 acgccgcctg cgaaatcgct cgcgatgcgg gccgcgccat cctcgaagtc tatgggcgcg
     1261 acgacttcgc cgtcgcgcgc aagagcgacg actcaccgct gacggaagcc gaccaggtcg
     1321 cgcacggcat catcaccggc gcgctggcaa agctcgacag cgcgctgccg atcctgtcgg
     1381 aagagtcgcg gccggccgag cacgcgacgc ggcgcagctg gccgcgctat tggctcgtcg
     1441 acccgctcga cggcacgaag gagtgcttga agcgcaacgg cgagttcacg gtcaacatcg
     1501 cgctcgtcga cgggcagcgc gccgtgctcg gcgccgtgca cgcgcccgtg ctcgaccgca
     1561 tgtactcggg agtggtcggc ctcggcgcat ggcgtagcga tgccggcgct gcgccgcggc
     1621 cgatcgcggt gcgcggcacg gccgcgacgc cgctgcgcgt cgtcggcagc cgcagtcatc
     1681 cgagtcccga gctcgccgcg tacttggccg gactgcccgc ccacgagatc accgacatgg
     1741 gcagctcgct gaagatttgc ctcgtcgccg aaggcgccgc agacgtttac ccgcggctcg
     1801 gcccgacgtc ggaatgggat accgccgccg cgcaggctat cctcgaaagt gcgggcgggc
     1861 gtatgatcga cctagccggc cggccgctgc ggtacaactc caaagacgac ctgttgaacc
     1921 cacatttcct ggcttttggc gaccaccggc gggactggtt ggcaccgatc cagaatacgc
     1981 gtggactaca aagtagaaac tgagactttc aggaacgtgg agcggctgaa gcaactgcgt
     2041 gttgagcatc gggacctcga ccaggtcatc gccaggctcg taaccgatcc caacgtggac
     2101 cagctgatgt tgaagcgtct gaagaagcgc aaactgatgc tcaaggacat gatcactcag
     2161 ctcgagagcg ccaggatccc ggacctcaac gcctgacggt gccctcgtgg atttgatggc
     2221 cgatatcggc gcaacgatga cccgttgcgc actcgtagga gagaaaggcc aagagctcgc
     2281 gcccgaaact ttcgagaacg ccgatttcac cggcgtggaa ggcgtgctgc gcgtttacct
     2341 cgatcatcga cgcgccagcg atcgtccctc gcgcgcggcg ctggcggttg cggcaccgat
     2401 cgtcggcgac gaagtccaga tgatcaacat cggctggcgc ttctcgcaga gccagctgca
     2461 gcaggcgttg gatctcaaac ggctgcaaat cgtcaacgac ttcgcagcga acgcgtgggc
     2521 gctgccgcag ctcgagccgg ccgacattgc cccgatcggc ggcggtgaga gcgcgccgcg
     2581 cacgacgctc gccgtgctcg gcccgggctc gggactcggc gttgccgggc tcgtgccgac
     2641 gggcgacggt tgggccgtga tgacgggcga aggcggccac gtttcgatgc cggccgcgac
     2701 acgcgaggag caggacgtca tcgcgctgtt acgcgatcgt ttcgacggcc attgctccgc
     2761 ggaaagggtc ttgtcgggac cggggctcgt gaatctctac gtcgcgctgg ccgagctcgc
     2821 ggggcgcggt catccgacgg tgaaccccga ggacgtcacg aatcttgcca ggcagggcga
     2881 gcccctcgcc cgcaagacgc tcgcgatgtt cttcgcgatg ctcggcacgg tggccgcgaa
     2941 cctcgcactg acggccggag cgcgcggcgg catttacatc gcgggcggca tcgtgccgcg
     3001 attcgtcgag cagctgcgaa aatcggagtt ccgcgcgcgg ttcgagaaca aaggccgtta
     3061 tcgttcctat ctcgcggcga ttccgacgca cgtgatcacg gcgccgttgc cggcgtttcg
     3121 cggcctcaag tatttgctcg gctaccgctg acgcggcgcg tcggtgccgc ttcgctcgat
     3181 ctcgctaacg ctccgcctcc tcgatgcggc ggcgctgctc ggccgcttgc tgatcgaccg
     3241 tttgctgcgc gctctgcgcg cgcccgatgg tgctcgtcag cggatcgaac accgtttcgc
     3301 gccgcggggc ggcctcacgg ctatccgacg cgcgctcttc tggctccgag ctgccgcacg
     3361 cggccagcgt cagtgcgagc gcggttgcac accacaagcc tgctccaagt cgcatcgggc
     3421 acctccggga cggcactgga gcttacaagc tagcagctcg ccttaggaaa aaccgagact
     3481 tgggacaccc ccggcgcggt tccgcgcgcc gcggaccgcg gtgtattctc gatctcctcg
     3541 ctacttcggg gcctcgaatg accagcgcca ctgcgcccgc caaggcatcg ctcgacccga
     3601 tcaaagtcct gtgctgggtt ggcatcgcgg cctatgtcgt cgtgaagttt ttgcgctggc
     3661 agcaaggcgc gacgctcggc gagctgttcg acttcggcga tccgtcgctg tgggggttga
     3721 tgttcggcgc ttgctgcgtc gggctcatct tgcgctcgcc gaagatccgc cggtcggcga
     3781 cgttgatcga ggcgaatgag acgcgcgccg tgacctggtc gttcctgacg ggcttcacgg
     3841 tgatgtcggc gtacttcttc ttgcggccgg ttcgcgactc gatggcgagc gattggtccg
     3901 acgccgagat ctctcacctt tggacgattc aattcttcct gagtttgggg ctcgtgatgg
     3961 tctatggcct cgcgtgctcg aagatccgct tccgctacct cgtccccgcc gtctacacgt
     4021 tctacgccgt gacgttcgcg ttgttctact tgggctcgaa ggccatgacc aacgcggtgt
     4081 tgctcgacaa gagcttctac gtctgggtca gcctgttcag cctgtttcat ctgtccgtgt
     4141 tctggagctt catggcggac accttcagct cggagcagtc gaagcgtcta ttcccgttca
     4201 tcggtgtcgg cgtgagcttg ggcgccgcgg ctgcgccgct cgtcgtcgcg gtgattgcca
     4261 gcgacgtcgg caatcaaaat ttgatgctcg tcgcggccgt gatcctgctg atcgcaattc
     4321 cgatgatctt ctatctgcag cggctcaaga gcgccgatct gcacaacgaa gacgtgcgcg
     4381 ccaacttggg cgccgcgacg atgggcggca aatggtggca gggcttccgc gatttcgcga
     4441 cgaacccgta tctgctgacg attggcgtgt tcatcctgct ctatacggcg atgcaggcgt
     4501 tcatctactt cgagacgacg gagctgttgc gcatctacga ggcgcgcgag gagcgcacga
     4561 cgatcttggc gctgcgcgac gccgtcgcca acatcctgac gttcgggctc ggcctcttcg
     4621 tgacgagccg actcgtcaaa cggctcggca tgccgtggac gttggcgtta ttgccggcgt
     4681 tttgcgcgct cggattcctg atcctggcga cggcgcccgt cctgtcggtc ctgctcgctg
     4741 tcgacatcgc gcgccgcggc ggcgagtacg gcatcacgag accggcacgc gagatcctct
     4801 tcacgagcgt tgaccgtgaa acgcgcttca agacgaagcc tgtcatcgac gtcgtcgtct
     4861 atcgaggcgg cgacgcatgg tggggcggca tcttcgctgc catgagcgaa ggcatcgggc
     4921 tcggcttcgc ggcgatgtcg ctgatcggct cggcatttgc tgccgtctgg atcgccgcat
     4981 cgctgtatct cggccgagcg ttcgagcggc gcgagcgtga cgtcgagtcg accgcaccgg
     5041 caccgacgcc gccgcgcgaa gccgtcgcgg gccaccgcgg ctagtagatt tcgcgcgtgt
     5101 agatcgtgcg gcggtcgccg ccgaagatgc cgaacgtggc ctggcccgtc gggtcttcgt
     5161 cgccggcggc acccgcgtcc caatcgaacc tgagccacgc aggcaccgcc gcgcgaacca
     5221 gcacgctgcc ctgattgccg gcgcccggcg cggccaaatt tagattgaaa tcgccggcta
     5281 gcggcggctc gcgataacga atcggcgcgg ccgccgccgc ggcacaaccc agggcgctcg
     5341 cgccgggcga tccactatcc cgcacgcagg tctcgccggc attcaagttc tcggcatagg
     5401 ccgggaacga cagcgagacg ttcgtcgtgc acgcgtcggc tgcgttcgtt acgaaacccg
     5461 cggcggcgct cgcgtaatac tcggctcgca ccggcacggg caagtcgacg agctccgagc
     5521 cgacggcggt gccgacgcgc acgcggccgt aacggatctc ctgccctgcc gtgaacggaa
     5581 tgccgcccgc ggcgccgaac gtcacggggt tgccgagcgg cgcggcgccg acggcggcca
     5641 cgccgtcggc gtcgagcacg ttgatcgcga gctgcacttg cgcgttgaac ggcgcctgcg
     5701 ccgcgccttt gacgaacgcg atgccgctgc cgctgctgaa cgtcagcgtc gcgacgccgc
     5761 cgctcgggct cgcaatcgcc gggtcgacgg ccgtcgcggg caagcctgcg acgttcagag
     5821 caccggcgac gctcgaatag ctgcgccccg tcagcgtcgt gttgctgagc ttgaagaacg
     5881 agcccgtgta attcgtcgtc gtcgagccgg agacgccgac ggccgtcgcc gtgatgacgg
     5941 gcggcgtcgt gtagccgaac gtctggcctt gatacgtgaa ggaagagcac gccgtcgcga
     6001 acagcggcga gttcagcacg gcgacgaaac ggctcggaat gaagcggccg atgcgctcgc
     6061 tggccgtgcc cgtcacgtcg ccggctgtca gataattcgc gtcgccgatg ccgggcaccg
     6121 ctcgcacgat gccgacttcc gaccacgtga aatcggtccc cgtcgccacg ccgttcgtga
     6181 acgagccgaa gcccgtcgtg taaccgatcg gcgggctcga gccgccggcc ggcgccacga
     6241 gctgcgtcgt cagccggacg gtttccggaa ccgactcgcg gccgaagttc ggcgtcgtgc
     6301 tgccttccgc gtcgcgcgcc gtaaccgtcg cccggaacgg ggcaccggcc gcgaggaaca
     6361 cggcaccggt tgcatcgggc gcttgcgggt tgacgaccgt gttggccgcg ttggcgatgc
     6421 cgctcaacaa aaaatcgtac ggccgcacga cgaagttcgc ggttgcgccc gtgatgcccg
     6481 agggcaactc ggcgttcacg gtcgagtcgt ccttcatcag cacgcggatg cggccgacgt
     6541 ccttgtactt cgcgacgacc gacgcttggc cgttcgtgaa cgtcacggct tgcgctactg
     6601 acgcgggttc cgacgccgcc gcgctcgcgc cgttgatcgt gacgttgcgc gtgcccgtgc
     6661 cggggtcgac gtactgcgac cagaacttca gattcttcgc gcccgtatag gcttcgatga
     6721 tgccgcacac cgagtcggtc ggcgtttggc cgtaggccgc gagatgcacc gtgaagttcg
     6781 tgccggccgt ctggttcgct gcgaactgcg tgaccgcgcc cggcgggttc gcgagggctg
     6841 ccgccgtgac cgtgaagccg ttcggcgaga acacgagcgc accttcggca tcgttgtccc
     6901 gaacgcccgt gttgctgatc tggaagacgt cgacgtcgag cgacggcggg ccttgcgggt
     6961 agtacagcgt gaacgtcgct tgcgactggc cgagcggcca cgtgtaggtc gcggcgccgt
     7021 cgccggccgt gccgtcgctg aacgcgcccg agcccgtcac gagggcccag gtgccgttgc
     7081 tgctctgtgt gtcgagctgc acggcggcgt tgtagttcac gagcggcgtg cccgtgatcg
     7141 agtcgatgac gttgaccgta atcgtctcgg gggcgcagtt gatgccaaac gcgttgtgcg
     7201 tgatcgtgaa ccgcgccgtg ttcgcacacg ggtgcgtctc gtcgcgcagc gcctgaatct
     7261 ccgcttgcgt cagcgcgtcg gcgacgatgt gcacttcgtc gatgtagccg tcgaaggcgc
     7321 gcgagatgaa gttccaatcg gttccgatgt acagcggcac ggtgttcgtc gcgagcgtgc
     7381 ccgtgaagtt accggtcgcg ccctgtagca cgccgttgat gtacatgcgc tgcgcgcccg
     7441 attggt
//
LOCUS       DATLEE010000660         4476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10924371, whole genome shotgun sequence.
ACCESSION   DATLEE010000660 DATLEE010000000
VERSION     DATLEE010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10924371"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /gene="rplM"
                     /locus_tag="VM692_14470"
     CDS             <1..296
                     /gene="rplM"
                     /locus_tag="VM692_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014030395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HUQ53428.1"
                     /translation="PHVDTGDFIVVINAEKIRVTGRKLKDKIYYRHTGTIGNMKSQPL
                     EKMLETHPERSIEIAVKGMLPKNSLGRQMFKKLKVVRGEKHPHGAQQPKPLEV"
     gene            300..692
                     /gene="rpsI"
                     /locus_tag="VM692_14475"
     CDS             300..692
                     /gene="rpsI"
                     /locus_tag="VM692_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HUQ53429.1"
                     /translation="MAIEINYGTGRRKTSAARVYLKPGNGDIVVNGRPLDVFFGRETA
                     RMIVRQPLVVAEAIDRFDVTVTVSGGGTTGQAGAIRHGITRALMDYDEGLRKTLRKAG
                     FVTRDAREVERKKVGLRKARRRPQYSKR"
     gene            750..823
                     /locus_tag="VM692_14480"
     tRNA            750..823
                     /locus_tag="VM692_14480"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:782..784,aa:Gln,seq:ctg)
     gene            1009..2637
                     /locus_tag="VM692_14485"
     CDS             1009..2637
                     /locus_tag="VM692_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53430.1"
                     /translation="MSASKSLPARPSLESLRKQAKKLARDVAAGDAAAIACMRAHLPR
                     IEPPLTQRNAQLVVAREYGFAGWQELAAEVGKRLGGGLEWAVAQAERAIHDDDVERLR
                     QLLGEYPSLKAWQRRKTNDNETDSLLGMATSAYGDAGDTERERWFTRASCAEALLDEG
                     ATVTQAVLEGLLQSRASGLLALFDRKGLLPRTLKFLAAVGDRDGVRAALASHAISVEA
                     VNEAFVCACRFDRAAVALALLERSIELDPALGRRIDASMERGAFVRACEKLDLAEVAE
                     LGLWKVFAIEQIQRTVHERNLAAFTDALRQESWLLGAASVRLQAGLLEMLAFQRDPQE
                     FVAALLDLDPAILRSRPPPASRAIDWAFMYDNTQLLPLLTRVWPVPDGVVFAAGLGDL
                     ARVRQWFDAAGVLRNPEQQYPYNDPDARSHLRWNPPTAQHVLDAALAFAVVNRHLDVA
                     DFLIERGANVSTDWGTHEPSSILHTVVFMPDNYGLMRFLIDRGIDMTIRDCRWNATAQ
                     GWALHALRDEKMAQWLAEAERQRELKRGPATESS"
     gene            complement(2621..3277)
                     /gene="rpiA"
                     /locus_tag="VM692_14490"
     CDS             complement(2621..3277)
                     /gene="rpiA"
                     /locus_tag="VM692_14490"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HUQ53431.1"
                     /translation="MTQDTNKHRAATAALSYIKDGMALGVGTGSTVDIFIELLQPLAA
                     KLGSVVSSSERSTRRLAEFGILTADLTSTGELDIYVDGADEATKHRHLVKGGGGALTR
                     EKIVAAASRRFVCIIDETKLVPTLGKFPVPVEVIPMAQSYVARQMVKAGGQPIWREGF
                     VTDNGNHIIDVHNLKIANPLELETRFNQIAGVVTVGIFANRPADVLLIGGASGIKTIQ
                     "
     gene            complement(3629..4096)
                     /locus_tag="VM692_14495"
     CDS             complement(3629..4096)
                     /locus_tag="VM692_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HUQ53432.1"
                     /translation="MSYWLMKSEPDVFGIDDLAKSPRKTTRWDGVRNFQARNFMRDQM
                     RRGDLAFFYHSNTEEPGIAGVVKIVKEGYPDHTAFDAADPHFDAQSDAAQPRWYMVDV
                     QLQRKFRQIVTLATLREHENRALKGLALLKRGNRLSVSPVSAAHWQFILGLAS"
     gene            complement(4113..>4476)
                     /locus_tag="VM692_14500"
     CDS             complement(4113..>4476)
                     /locus_tag="VM692_14500"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HUQ53433.1"
                     /translation="VLYVPVLHGMTMTFAELEADGTLAPNSFGILEPKLGAKIDARQL
                     DVVLTPLVGFDDRGVRIGVGRGYYDRCFRFLRHRAHWRRPKLLGVAYELQRVPPLVPS
                     SWDVPLWGIVTERGVQRF"
BASE COUNT          794 a         1437 c         1412 g          833 t
ORIGIN      
        1 cgccgcacgt cgacacgggc gacttcatcg tcgtaatcaa cgccgagaag atccgcgtca
       61 cggggcgcaa gctcaaggac aagatctact atcgccacac gggcacgatc ggcaacatga
      121 agtcccagcc gctcgagaag atgctcgaga cgcatcccga gcgctcgatc gagatcgccg
      181 tcaaaggcat gttgccgaag aactcgctcg gccggcagat gttcaagaaa ctcaaggtcg
      241 tgcgcggcga gaaacatccg cacggcgcac agcagccgaa accgttggaa gtctaagaca
      301 tggccatcga aatcaattac ggcaccggac gacgcaagac ctcggcggcc cgcgtatacc
      361 taaagcctgg caacggcgac atcgtcgtca acgggcggcc tctcgacgtg ttcttcggcc
      421 gcgagacggc gcgtatgatc gtgcgccagc cgctcgtcgt cgccgaagcc atcgaccgct
      481 tcgacgtcac cgtcacggtc agcggtggcg gcacgacggg ccaagccggc gcgattcgcc
      541 acggcatcac gcgcgcgctg atggactacg acgaaggcct gcgcaagaca ctgcgcaagg
      601 ccggtttcgt gacgcgcgac gcgcgcgagg tcgagcgcaa gaaggtgggc ctccgcaagg
      661 cgcgccgacg cccgcagtac tcgaagcgct agaattcgta tcgcgcagcg cgccggctcg
      721 gccggccgtt cgttctgctg aaatcacact ggggtgtggt gtaacggtag cacagcagac
      781 tctgactctg tttgtcgagg ttcaaatcct tgcaccccag ccagcacttc tcggtgcttt
      841 gactcccgag cgccggtcgc tcgatactgt taggtagcgc gctcacaggg cgccacctac
      901 ccgcggcaag ggaatactcc cacctgtttc gccctcgagt tgcgtgcgat tgcaccttct
      961 ttcagcctgg tctcagcggg gtacaggcac ccatgaaagg aggttgccat gtccgcttcc
     1021 aagtccctgc ctgctcgtcc gtccctcgag tcccttcgta agcaagccaa gaagctcgcg
     1081 cgcgacgtcg ctgccggcga tgccgctgcg atcgcgtgca tgcgtgcgca tctgccgaga
     1141 atcgagccgc cgctgacgca gcgcaatgcc cagctcgtcg tcgcgcgcga gtatggattc
     1201 gccggctggc aggagctcgc ggccgaggtc ggcaaacgcc tcggcggagg gctcgaatgg
     1261 gcggttgcgc aagccgagcg tgcgattcac gacgacgacg tcgagcgctt gcggcagctg
     1321 cttggcgaat atccgtcgct gaaggcgtgg cagcgccgca agaccaacga caacgaaacc
     1381 gacagcctgc tcggcatggc cacgagcgcg tatggcgacg cgggcgacac ggagcgcgaa
     1441 cgctggttca cgcgcgcgtc gtgcgcggaa gcgctgctcg atgaaggcgc cacggtgaca
     1501 caagcggttc tcgagggact gctgcaatcg cgcgcgagcg gtctcttggc gttgttcgac
     1561 cgcaaaggtc tgttgccgcg cacgctgaag ttcttggcgg ccgtcggaga tcgcgacggc
     1621 gttcgcgcgg cgctcgcttc gcacgcgatc tccgtcgagg ccgtcaacga agcgttcgtg
     1681 tgtgcgtgcc gcttcgaccg cgcggcggtc gcgcttgcgt tgctcgaacg gtcgatcgag
     1741 ctcgatcccg ccttgggccg acgtatcgac gcgagcatgg agcgcggcgc gttcgtaaga
     1801 gcttgcgaga agctcgacct cgccgaggtc gccgagctcg ggctgtggaa ggtgttcgcc
     1861 atcgagcaga ttcagcgcac ggtccatgag cggaatctcg cagccttcac cgacgcgctg
     1921 cggcaggagt cgtggctgct cggcgccgcg tcggttcgcc tgcaggcagg gctcctcgag
     1981 atgttggctt ttcagcggga cccgcaggag ttcgtcgcgg cgctactcga cctcgacccg
     2041 gcaatcctac ggtctcggcc gccgcctgcg tcgcgcgcga tcgattgggc tttcatgtac
     2101 gacaacacgc agctgctgcc gctgctgacg cgcgtttggc cggtgcccga cggcgtggtg
     2161 ttcgcggcgg gtttgggcga cctcgcgcgc gtccggcaat ggttcgacgc cgccggcgtg
     2221 ctgcgcaatc ccgagcagca gtatccgtac aacgatcccg acgcgcgcag ccacttgcga
     2281 tggaatccgc cgacggcgca acacgtgctc gacgcggcgc tcgcgtttgc cgtcgtcaac
     2341 cgccatctcg acgtcgccga cttcctcatc gagcgcgggg cgaacgtcag caccgattgg
     2401 ggcacgcatg agccatccag catcctgcac accgtcgttt tcatgccgga caactacggc
     2461 ttgatgcgct tcctcatcga tcgcggcatc gacatgacga tcagagactg ccgctggaat
     2521 gccacggcgc agggttgggc gttgcacgcc ttgcgcgacg agaagatggc ccagtggctc
     2581 gcggaagcgg agcggcagcg tgagctgaag cgcggtccgg ctactgaatc gtcttgatgc
     2641 cgctcgcgcc gccgatcagc agcacgtccg cgggccgatt cgcgaagatc ccgaccgtca
     2701 cgacgccggc gatctgattg aagcgtgtct cgagctcgag cgggttcgcg atcttcaaat
     2761 tgtgcacgtc gatgatgtgg ttgccgttgt cggtcacgaa cccttcgcgc cagatcggct
     2821 gaccgccggc cttgaccatc tggcgcgcga cgtacgactg cgccatcgga atcacttcga
     2881 ctggaaccgg aaacttgccg agcgtcggca cgagcttcgt ctcgtcgatg atgcagacga
     2941 agcggcgcga cgccgcggcc acgatcttct cgcgcgtcag cgcgccgccg ccgcccttca
     3001 ccaaatgcct atgtttcgtg gcctcgtcgg cgccgtcgac gtagatgtcg agctcgcccg
     3061 tagaggtcag atcggccgtg aggatgccga attcagcgag ccgccgcgtc gagcgctcgg
     3121 agctcgacac cacggagccg agcttcgcgg caagcggctg caatagctcg atgaaaatgt
     3181 cgaccgtgct gcccgtaccg acgccgagcg ccattccatc tttgatgtac gacaacgcag
     3241 ccgtcgctgc tcgatgcttg ttcgtgtctt gagtcatcaa agtgcccttc ccaaaaagag
     3301 agtgcccgca acttggcggg cactacactc gtttgagcta ttcgctctta acgttttttg
     3361 cgtcgcgttt tgcgcttcgc tttcttcgcg ccgcgcttcg ccggacgctt cgcagctttg
     3421 cgcttcttac gtgatgcttt cttcctagag gccatgggta gtctccgttg tcgggataga
     3481 cggcgttgag tatatataaa ttaaatagga tttccagcgc gttataaatt tttgcgcatc
     3541 tttgtcgtgc acgtgcacgt gcggagtgtg attggttgca cactcttgca cgtcgttcga
     3601 cgaacgaagt tgtcgcgcgc atcgtgcgtc acgacgcgag accgagaatg aattgccaat
     3661 gcgcggcgct caccggcgac actgacaagc gattgccgcg cttcaatagt gcgagccctt
     3721 tgagcgcgcg attctcgtgc tcgcgcagcg ttgcaagcgt cacgatctgg cggaatttgc
     3781 gctgcaactg cacgtcgacc atgtaccagc gcggctgggc ggcatcgctc tgcgcgtcga
     3841 agtgcggatc agcggcgtcg aatgccgtgt gatcgggata accttccttg acgatcttca
     3901 cgactcccgc gatgccgggt tcttccgtgt tcgagtgata aaagaatgcg aggtcgccgc
     3961 ggcgcatctg gtcgcgcatg aagttgcgcg cctggaagtt gcgaacgccg tcccaacgcg
     4021 tcgtcttgcg cgggctcttg gccagatcgt cgatgccgaa cacgtcgggc tcggacttca
     4081 tcagccaata gctcatcggt ccaagccttc catcaaaacc gctgcacgcc gcgttccgtg
     4141 acgattcccc atagcgggac gtcccagctg ctcggcacga gcggcggcac gcgttgtagc
     4201 tcgtacgcca cacccagcag cttcgggcgc cgccaatggg ccctatgccg caggaaacgg
     4261 aagcagcgat cgtaatagcc gcggccgacg ccgattctca cgccgcgatc gtcgaatccg
     4321 actagcggcg tcaagacgac gtcgagctgg cgcgcgtcaa ttttggcgcc aagcttcggt
     4381 tccaggatgc cgaacgagtt tggcgccagc gtgccgtccg cctcgagctc ggcgaacgtc
     4441 atcgtcatgc cgtgcaacac gggaacgtat agaacc
//
LOCUS       DATLEE010000661         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8692266, whole genome shotgun sequence.
ACCESSION   DATLEE010000661 DATLEE010000000
VERSION     DATLEE010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8692266"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VM692_14505"
     CDS             complement(<1..907)
                     /locus_tag="VM692_14505"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUQ53434.1"
                     /translation="MPIERVTVVVIGAGHTGLAVSRMLTERAIEHVVLERGDVANSWR
                     RERWDSLRLLTPNSQSRLPGYAYSGGDPDGFMTANEVVDFIGGFASAIDAPVRTQTEV
                     IRVQPADDSYRVATAHGSELRARNVVIASGACNVPSVPALRAAVPSSVASFTPFDYRN
                     PSTLPEGGVLVVGASATGVQLADELRRSGRRVLLSVGEHVRLPRTYRGRDVLWWMEAS
                     GIWNQRYDEIDDVERARNLPSPQLVGTPQRATLDLNALSAQGVEIVGRLAAIRDGRAL
                     FSGGLRNNFALADLKMNRLLESFDGW"
     gene            complement(946..1509)
                     /locus_tag="VM692_14510"
     CDS             complement(946..1509)
                     /locus_tag="VM692_14510"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUQ53435.1"
                     /translation="MATAVDNGVNVGALLEAREALRKMPEAAKFTWRATCKWKKGTYS
                     KTRIDNFFGLGQEQKHRTEFTFEADHPEIFASEDCGATPVELVLAGLASCLTAGVAAV
                     AQNRGVQLRSVEAKLEGSMDIQGILGIDSDVRNGYDGIKVTFDIDADASQKDIEAIVA
                     QSQKRSAVYDIVTNPTDVTVEVRKKKR"
     gene            1602..>2363
                     /gene="metX"
                     /locus_tag="VM692_14515"
     CDS             1602..>2363
                     /gene="metX"
                     /locus_tag="VM692_14515"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HUQ53436.1"
                     /translation="MAARAKDDAVGSVGTVETRFLDLPNPLRLDCGRDLHTIRIAYET
                     YGTLAPKRDNVILVCHALSGDAHAAGYSSAPTAAGTRDGFGAEDRDAERGRSLGWWDG
                     MIGPGKAFDTNRFFVVSTNLLGGCRGTTGPSSPNPATRRPYGSDFPVITVADMVRAQR
                     AFLNELGLERLAAIAGGSLGGMQALEWAVQFPDDIDRIVPIASTARLGAQGVAWNAIA
                     RNAIMSDPDWDGGDYEGTGRKPDLGIKVARMVGHVT"
BASE COUNT          368 a          826 c          798 g          371 t
ORIGIN      
        1 cccagccgtc gaacgattcg agcaggcggt tcatttttag gtccgcgagc gcgaagttgt
       61 tacgcaagcc gcccgagaac agcgcgcggc cgtcgcggat cgcggcgagc cgcccgacga
      121 tctcgacgcc ctgcgcgctc agcgcgttga ggtcgagcgt cgcgcgctgc ggcgtgccga
      181 cgagctgcgg cgacggcaga ttgcgcgcgc gctccacgtc gtcgatctcg tcgtagcgct
      241 ggttccaaat gccgctcgcc tccatccacc agagcacgtc gcggccgcgg tacgtgcgcg
      301 gcaagcgcac gtgctcaccg accgagagca gcacgcgccg gccggagcgc cggagctcgt
      361 ctgcgagctg cacgccggtc gccgacgcgc cgacgacgag cacgccgccc tcgggcaacg
      421 tcgaaggatt gcggtagtcg aacggcgtga acgacgccac ggacgacggc acggcggcgc
      481 gcagcgcggg cacactcggc acgttgcagg caccgcttgc gatgacgacg ttgcgcgcgc
      541 gcagctcgct gccgtgcgcg gttgcaacgc gatagctgtc gtcggccggc tgcacgcgta
      601 tgacctcggt ctgcgtgcgg accggcgcgt cgatcgcgga agcgaagccg ccgatgaagt
      661 cgacgacctc gttcgctgtc atgaagccgt cgggatcgcc gcccgagtat gcgtagcccg
      721 gcaaccggct ctgcgagttc ggcgtcagca gccgcaacga atcccagcgc tcgcggcgcc
      781 aggaattcgc gacgtcgccg cgctcgagca cgacgtgctc gatcgcacgc tccgtgagca
      841 tgcggctcac ggccaggccg gtgtggcccg cgccgatgac gactacggtg acgcgttcga
      901 tcggcaaggg ctcgacgatt tccggaaggg cgccgcgcta gaggatcagc gcttcttctt
      961 gcggacctcg accgtgacgt ccgtcgggtt cgtgacgatg tcgtagacgg ccgagcgctt
     1021 ctgcgactgc gcgacgatgg cctcgatgtc cttctgtgac gcgtcggcgt cgatgtcgaa
     1081 cgtgaccttg atgccgtcgt agccgttacg cacgtcgctg tcgatgccga ggatcccctg
     1141 gatgtccatc gaaccttcga gcttcgcctc gacggagcgc agctgcacgc cgcggttctg
     1201 cgcgacggcc gcgacacccg cagtaagaca gctcgcaaga cccgcgagca cgagctcgac
     1261 gggcgtcgcg ccgcaatcct cggacgcgaa gatctcggga tggtccgctt cgaacgtgaa
     1321 ctcggtgcgg tgcttctgct cctgaccgag cccgaagaaa ttgtcgatgc gcgtcttgct
     1381 gtacgtgccc ttcttccatt tgcacgtcgc ccgccacgta aactttgccg cctcgggcat
     1441 cttgcgcagc gcttcgcgcg cctcgagcag cgcacctacg ttcacaccgt tgtctaccgc
     1501 cgtcgccatg ggtgactcct gttcgttatg caaagggtcg agggatgata gccccgcgtc
     1561 gccaccagtg tctaagcgcc cgccgacgta tactgcggcg catggccgcg cgcgcgaagg
     1621 atgacgcagt cgggtccgtc ggcacggtcg agacccgctt cttggacctg ccgaacccgc
     1681 tgcggctcga ctgcggccgc gacctccaca ccattcgcat cgcgtacgaa acctacggca
     1741 cgctcgcgcc gaagcgcgac aacgtcatcc tcgtctgtca tgcgctctcg ggcgacgccc
     1801 acgcagccgg ctactcgagc gcgcccacgg ccgcggggac gcgcgacggc ttcggcgccg
     1861 aggaccgcga cgccgaacgc ggccgcagct tgggctggtg ggacggcatg atcggccccg
     1921 gcaaagcgtt cgacacgaac cgcttcttcg tcgtcagcac gaacttgctc ggcggctgtc
     1981 gcggcacgac ggggccgtcc tcgccgaatc ccgcgacgcg gcggccgtac ggctccgact
     2041 tcccggtgat caccgtcgcc gacatggtgc gcgcgcagcg cgcgttcttg aacgagcttg
     2101 ggctagaacg gctcgcggcc atcgccggcg gctcactcgg cggcatgcag gcgctcgagt
     2161 gggcggtgca gttccccgac gacatcgacc gtatcgtccc gatcgcgtcg acggcgcgcc
     2221 tcggagcgca aggcgtcgcc tggaacgcga tcgcgcgcaa cgcgatcatg tcggaccccg
     2281 actgggacgg cggtgactac gaggggacgg gacgcaagcc cgacctgggc atcaaggtgg
     2341 cgcgcatggt cggacacgtg acg
//
LOCUS       DATLEE010000662         3869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10924796, whole genome shotgun sequence.
ACCESSION   DATLEE010000662 DATLEE010000000
VERSION     DATLEE010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10924796"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1344)
                     /locus_tag="VM692_14520"
     CDS             complement(106..1344)
                     /locus_tag="VM692_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ53437.1"
                     /translation="MDSPPAARSRRDLKLIKALTCTMFFTFAMTTDAVGSIIPTLLEE
                     FALSLRAASAFHYVPMIAIAAGALALGFLADRLGRKSTIVLGLAIYGVGCLLFAFGSG
                     FGFFVVLLGVGGIGVSVFKIGALALIGDISRSTREHTTFMNTVEGFFAVGAIVGPAIV
                     ATLLAAGLSWKYLYVIAAVICAVLIVAAARARYPSTGAQNGGASFVRTLSKMRDAHAL
                     GFSTLIMLYVAVEVAVYVWMPTYLQGYAGPTAWLAAYALTIFFVLRAAGRFLGAWFLR
                     KLSWSTVLVVFAAAIVVCFAGSLYGGPDHAVYLLPLSGLFMSVIYPTLNSKGISCFPR
                     SEHGAVAGVILFFTAFAAAVGPLAMGAVSDALGDIKYGFVLATGFAALLFAGLLVNRV
                     FDPAGARLASRDDSDYAPAH"
     gene            1446..2969
                     /gene="gtfA"
                     /locus_tag="VM692_14525"
     CDS             1446..2969
                     /gene="gtfA"
                     /locus_tag="VM692_14525"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HUQ53438.1"
                     /translation="MKNAVQLITYVDRLSGGGFADLTALLEGPLRGLFAGVHVLPFFD
                     PIDAADAGFDPCDHTRVDPRLGDWSDVAALAAGTDVMADLIVNHISRDSPQFRDFLTQ
                     GSASPFAELFLTRERVFPHGATDAELQAIYRPRPGLPFTAMTVGDGSRRHLWTTFTPS
                     QIDIDVTHARGREYVDAILAKLAASGVRVVRLDAVGYAVKKRGTSCFMIPETYELIAD
                     LSRRVRALGMETVVEIHSHYRQQIEIAGQVDWVYDFALPPLVLHAFAFGTARPLASWL
                     RMRPHNALNVLDTHDGIGIVDIGAERGAVSKPGLVQPGELDALVESIHTASAGMSRLA
                     TGGAASNLDLYQVNCTFYDALARVDRDYLLARAIQFFTPGIPQVYYVGLLAGHNDLEL
                     LRHSGVGRDINRHYYGRAEVTAALQRPVVRNLCALIRLRNAHPAFRGEFSSDVSADTE
                     LHMRWRNGVERAELRIDFRSRHCELALSEAGAMRSLEITELADATPERSPLKAATSA"
     gene            complement(3008..3790)
                     /locus_tag="VM692_14530"
     CDS             complement(3008..3790)
                     /locus_tag="VM692_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53439.1"
                     /translation="MRAVSVLAAFAFSLAAAPIEVSAQAPQGHGIAGFWSMAPGPTPP
                     RRAATPAEAALIAQLRAGAHLLADAGATEFPPGDYGGLNVHASLREAAKDYDPENQTD
                     VSLTCRPPGVIYSMQGPFPIEIFEGRDLVVIKLEYFDLVRIIFMNETQHPDDWPHSQT
                     GHSIGRWEGETLVVDTAKLLAGTLFNNGVDYTEAVHLSERFRLADENTLVVTQQFEDP
                     GSFEGTAARVMSFRRGDDHVYPYDCDPTYGLAIDSRGGPGRE"
BASE COUNT          716 a         1306 c         1257 g          590 t
ORIGIN      
        1 ttcgccgcgc cagcgcgaag cgtcggctga gccgacgcgg aaaagttcgc agctgccgct
       61 gccgcaacca ccacgaacaa aggccaagtc ttcatgagtg gatcctcagt gcgccggggc
      121 gtagtcggaa tcgtcgcggc tcgccaggcg cgcgccggcc gggtcgaaca ctcggttgac
      181 gagcaagccg gcgaacaaca acgccgcgaa gcccgtggcg agcacaaagc cgtacttgat
      241 gtcgccgagg gcgtcgctga cggcgcccat cgcaagcggc ccgaccgcag ccgcgaaagc
      301 cgtgaagaac aagatcacgc cggcgacggc cccatgctcg gaacgcggaa agcagctgat
      361 gcctttcgag ttcaacgtcg ggtagatgac cgacatgaac aagccggaca gcggcagcag
      421 atatacggcg tggtcgggac cgccatagag gctgcccgcg aaacagacca cgatcgcggc
      481 agcgaacacg acgagcacgg tgctccacga cagcttgcga agaaaccagg cgccgagaaa
      541 gcggccggcg gcacgcagga cgaagaaaat cgtcagcgca tacgccgcca gccaagccgt
      601 cgggcctgcg tagccctgca ggtacgtcgg catccagacg tagacggcga cctcgacggc
      661 gacgtacagc atgatcaacg tcgagaagcc gagcgcgtgc gcgtcgcgca tcttgctgag
      721 cgtccgaacg aacgacgcgc cgccgttctg cgcccccgtg gacgggtagc gggccctggc
      781 ggccgccacg atgagcaccg cgcaaatgac ggccgcgatc acgtagagat acttccacga
      841 cagccccgct gcgagcagcg ttgcaacgat cgccgggccg acgatggcgc cgaccgcaaa
      901 aaatccttcg acggtattca tgaacgtcgt gtgctcgcgc gtcgagcgcg agatgtcgcc
      961 gatcaacgcg agcgcgccga tcttgaacac gctgaccccg atgccgccga cgccgagcag
     1021 cacgacgaag aagccgaagc cgctgccgaa agcaaacagc aggcagccga cgccgtagat
     1081 cgcgagaccg aggacgatcg tagacttacg gccgaggcgg tccgcgagaa atcccaaagc
     1141 cagtgcgccg gcggcgatcg cgatcatcgg cacgtagtgg aacgcgctcg ccgcacgcag
     1201 cgacagagcg aactcctcga gcaaagtcgg gatgatgctg ccgacggcgt ccgtcgtcat
     1261 cgcaaacgtg aagaacatcg tgcacgtgag ggccttgatc agcttgaggt cgcggcggct
     1321 acgtgcagca ggcgggctgt ccatcgatcc cccaatccag aacacgagcg agcaaagccc
     1381 ggctttacgc ctattgcaaa cgattgcatc atagcgacgg cccgccgctc gcgtaaacgg
     1441 ttccgatgaa gaacgccgtc cagctgatca cgtacgtgga ccgtctgtcg ggaggcggtt
     1501 ttgccgacct cacagccctg ctcgaggggc cgctacgcgg cctgttcgcc ggcgttcatg
     1561 tgctgccgtt cttcgatccg atcgatgcag ccgacgcggg cttcgacccg tgcgaccaca
     1621 cgcgcgtcga tcccaggctc ggcgactgga gcgacgtcgc ggcgctcgct gccggcaccg
     1681 acgtcatggc cgacttgatc gtcaaccaca tctcgcgcga ctcgccgcaa tttcgcgatt
     1741 tcctgacgca gggatcggcg tcgccgtttg cggagctgtt tctgacgcgc gagcgcgtgt
     1801 ttccgcacgg cgcaaccgac gccgagctgc aggcgatcta tcgcccgcgg ccgggattgc
     1861 cgttcacggc gatgacggtc ggcgacggca gtcgccgcca cctgtggacg acgttcacgc
     1921 cgagccagat cgacatcgac gtcacgcatg cgcgcggccg cgagtacgtc gacgcgatcc
     1981 tcgcgaagct cgccgcaagc ggcgtgcgcg tcgtgcggct cgatgccgtc ggctacgccg
     2041 tcaagaagcg cggcacgagc tgcttcatga tcccggagac ttacgagctc atcgccgacc
     2101 tgtcgcggcg cgttcgcgcg ctcggcatgg agacggtcgt cgaaatccac tcgcactatc
     2161 gccagcagat cgagatcgcg ggccaggtcg actgggtgta cgactttgcg ctgccgccgc
     2221 tcgtgctgca cgcgttcgcg ttcggcacgg cgcgaccgct ggcaagctgg ctgcgcatgc
     2281 gcccgcacaa cgcgctcaat gtgctcgaca ctcacgacgg catcggaatc gtcgacatcg
     2341 gcgcggagcg cggcgccgtg agcaagccgg gcctcgtgca gcccggcgag ctcgacgcgc
     2401 tcgtcgagtc gatccacacg gcgagcgccg gcatgagccg actcgcgacg ggcggcgcgg
     2461 catcgaacct cgatctgtat caagtcaact gcacgttcta cgacgcgctt gcccgagtcg
     2521 accgcgatta cttgctcgcg cgcgcgattc aattcttcac gcccggtata ccgcaggtct
     2581 actacgtcgg tttgctcgcc ggccataacg acctcgagct gctgcggcat tccggtgtcg
     2641 gccgcgacat caaccgccac tattacggac gcgccgaggt gactgcagcg ctgcaacgcc
     2701 cggtcgtccg caatctctgt gcgctgattc ggctgcgcaa cgctcacccg gcctttcgcg
     2761 gcgagttttc gagtgatgtc tcggccgata ccgagcttca catgcggtgg cggaacggcg
     2821 tcgagcgcgc ggagctacgc atcgacttcc gcagccgcca ctgcgagctc gcgctatccg
     2881 aggccggcgc catgcgctcg ctcgagatca cggagctcgc cgacgcgaca ccggagcgca
     2941 gcccgcttaa agcggcgacg agcgcctaga agcaccggct gctgctgcgc cttgcgaacc
     3001 ggccgcccta ctcccggccg ggaccgccgc gcgaatcgat cgcgagaccg taggtgggat
     3061 cgcaatcgta cgggtagacg tgatcgtcac cgcgccgaaa cgacatgact cgcgcggccg
     3121 ttccctcgaa agagcccgga tcctcgaact gctgagtgac gaccaacgtg ttctcgtcgg
     3181 cgaggcgaaa gcgttcggaa agatggacgg cttccgtgta gtcgacgccg ttgttgaaca
     3241 acgtgccggc cagcagcttc gccgtgtcga cgacgagcgt ctcgccttcc cagcggccga
     3301 tcgaatggcc ggtttgcgag tgcggccaat cgtcgggatg ctgcgtttcg ttcatgaaga
     3361 tgatgcgcac gaggtcgaag tactcgagct tgatgacgac gaggtcgcgg ccttcgaaga
     3421 tttcgatggg gaatgggccc tgcatcgaat agatcacgcc gggcggccgg cacgtcagcg
     3481 acacgtcggt ctggttctcg ggatcgtaat cctttgcggc ttcgcgcaag ctcgcgtgca
     3541 cgttcaagcc gccgtagtcg ccgggcggaa actccgtcgc gcccgcgtcg gcgagcaagt
     3601 gagcgcccgc gcggagctgc gcgatcagcg cggcctccgc cggtgtcgcg gcacgccgcg
     3661 gcggcgtcgg ccccggagcc atggaccaga aacccgcgat gccgtggcct tgcggcgcct
     3721 gcgcgctcac ctcgatcggc gctgccgcga gactgaaggc gaacgcggcc aagacactca
     3781 ccgcgcgcat cagttgttcg ttcccggcgg cacgccgatc tccgttccgt cttcgcgcca
     3841 tgcgcgcgtg atgcgcatca gataactgc
//
LOCUS       DATLEE010000663         3635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6390539, whole genome shotgun sequence.
ACCESSION   DATLEE010000663 DATLEE010000000
VERSION     DATLEE010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6390539"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VM692_14535"
     CDS             complement(<1..860)
                     /locus_tag="VM692_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HUQ53440.1"
                     /translation="MKKFVNDPAKFVPEMLEGIFLANEGKLKYEPKYNLIYRADRPSK
                     NHVSVIQGSGSGHEPAHVMTVGRGMLDAASPGNVFAAPPMEYCLECIKLMNSPKGVLV
                     LINNYQGDRMNWDMAIEMAEAEGINVKSFIIDDDVAVQNSSYTIGRRGVAGNFFVMKA
                     CGAAAEKGATLEQLLEIAKKVNANVRSMGIALTSCTPPAKGKPIFELGDDEMEVGVGI
                     HGEPGRRRDKMRPADAIVDELFDAVAKDLPFKSGDRVALMINGLGGTPPSELYVLYRQ
                     AALRSKTAGL"
     gene            complement(886..1551)
                     /gene="dhaL"
                     /locus_tag="VM692_14540"
     CDS             complement(886..1551)
                     /gene="dhaL"
                     /locus_tag="VM692_14540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HUQ53441.1"
                     /translation="MKEITLADVETAVKSIATTALKNEQYFCELDSYAGDADFGVSLA
                     TGFRAIEQEWSALDRSSIGSFLLKISVVISKHVGGCSGPIWGTAFMRAGMQSKDRTTI
                     TLDDLDKMLTASIEGIQKRGGAQLGDKTLLDALIPIRDIVHESAGKPDANAREVLQRA
                     TAVANEAIERTKGLVAKRGRQQFVGDRSIGTPDPGIVAIATMMNDLCTQFGVAPPASQ
                     SKN"
     gene            complement(1573..2289)
                     /locus_tag="VM692_14545"
     CDS             complement(1573..2289)
                     /locus_tag="VM692_14545"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUQ53442.1"
                     /translation="MKALIFDCDGVLVDTERDGHRVAFNRAFAEAGIRAEWDVKLYGE
                     LLKIAGGKERMKHYFDIKGWPPGKTAETLIPELHKKKTAIFTALIAGGSLPLRPGIAR
                     IVDEAHAAGIRLGVCTTSDPKSIDGVLNLLGAKRKSWFEIVLAGDIVQKKKPDPEIYV
                     LAKQRLGLDSPDCVVVEDSRNGLLASLGAGMPTLITTSTYTKGEDFKGAARVVPELGD
                     VPKVLVTLEDLKAIAAQCAA"
     gene            complement(2312..3181)
                     /locus_tag="VM692_14550"
     CDS             complement(2312..3181)
                     /locus_tag="VM692_14550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HUQ53443.1"
                     /translation="MPLVNLKPVLREAKAKGYAVAAFNPVDYASMKAMVKAAEELNAP
                     VIIQSSAKTIKYYGHKALIGWMREIADDSPVPVVLHLDHGKDVEMIKKCVEMGWTSVM
                     IDWSDKPFDVNLEQTKKVVDLATRAGIGVEAEIGEIHGVEDDMVVDAVHYTDPNEAER
                     FCRELDLAAFAAAIGTAHGYYKGEPDVKFELIGEINRRTNTPMALHGGTGLNDEIIQK
                     CIQLGCAKINISTNLKHVFIDSFIDYHQKNKKYEPLNVLGAQYEALKQLFKEKIAQFG
                     GKNRGSEVLSKVA"
     gene            complement(3302..>3635)
                     /locus_tag="VM692_14555"
     CDS             complement(3302..>3635)
                     /locus_tag="VM692_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015132458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HUQ53444.1"
                     /translation="YVHRIGRTGRAGNEGLAISLVAHDERPLLKQIERLLGYSLPTLT
                     PQGYVPGPPRADERPQRHHEHRQQPGRNNERRGNRGSQPRHEGHRHGGRNQRRGSRPP
                     GRGQGGGR"
BASE COUNT          570 a         1167 c         1134 g          764 t
ORIGIN      
        1 ttgagtcccg ccgtcttcga gcgcagcgcc gcctggcggt acagcacgta gagctcgctc
       61 ggcggcgtgc cgcccaagcc gttgatcatc agcgcgacgc gatcgccgct cttgaacggc
      121 agatctttgg cgacggcatc gaacagctcg tcgacgatcg catccgcggg tctcatcttg
      181 tcgcggcgac ggcccggctc gccgtgaatg ccgacgccga cttccatctc gtcgtcgccg
      241 agctcgaaga tcggcttgcc cttggccggc ggcgtgcacg aagtcagcgc aatgcccatc
      301 gaacgcacgt tcgcgttgac cttcttggca atctcgagca actgctcgag ggttgcccct
      361 ttctcggcgg ccgcgccgca agccttcatg acgaagaaat tgccggcgac gccgcggcgg
      421 ccgatcgtgt aagagctgtt ctgcacggcg acgtcgtcgt cgatgatgaa cgacttgacg
      481 ttgatgccct cggcctcggc catctcgatg gccatgtccc agttcatgcg atcgccctga
      541 tagttgttga tcaagacgag cacgcctttc ggcgaattca tgagcttgat gcactcgagg
      601 caatactcca tcgggggcgc ggcgaacacg ttgcccggcg acgccgcgtc gagcatgccg
      661 cggccgactg tcatgacgtg ggccggctcg tgaccgctgc ccgagccttg aatcacggac
      721 acgtgattct tgctcggacg gtcggcgcga tagatgagat tgtatttcgg ctcgtacttg
      781 agcttgccct cgttggccag aaaaatgccc tcgagcatct ccggaacgaa cttggccgga
      841 tcgttgacga acttcttcat gatggttccc ccaatcgttg acgggtcagt tcttgctctg
      901 gctcgccggc ggtgcaacgc cgaactgcgt gcacaagtcg ttcatcatcg tcgcgatggc
      961 gacgatgccg gggtccggcg taccgatgct gcgatcgccg acaaattgct ggcggccgcg
     1021 ctttgcgacg agacccttcg tgcgctcgat ggcttcgttg gcgacggcgg tggcgcgttg
     1081 caggacttca cgcgcgttgg catcgggctt ccctgcgctc tcgtgcacga tgtcgcggat
     1141 cggaatcagc gcgtcgagca gcgtcttgtc gccgagctgc gcgccgccgc gcttctgaat
     1201 gccctcgatg ctggccgtca gcattttgtc gaggtcgtcg agcgtgatcg tcgtgcggtc
     1261 cttggactgc ataccggcgc gcatgaaggc cgtgccccag atcgggcccg agcagccgcc
     1321 gacatgcttc gagatgacga cgctgatctt gagcaagaag ctgccgatcg agctgcgatc
     1381 gagcgcgctc cattcctgct cgatcgcgcg gaagcctgtg gccagcgaca cgccgaaatc
     1441 ggcgtcgccg gcgtatgagt cgagctcgca gaagtattgc tcgttcttga gcgccgtcgt
     1501 cgcgatcgat ttcaccgccg tttcgacgtc cgccagggtg atctctttca tcgtctctcc
     1561 tctggcacca ggtcatgcgg cgcattgtgc tgcgatcgcc ttcaagtcct cgagcgtgac
     1621 gagcaccttc ggaacgtcgc cgagctctgg cacgactcgc gcggcgcctt tgaagtcttc
     1681 gcccttcgtg tacgtgctcg tcgtgatcag cgtcggcatg ccggcgccga gcgaagcgag
     1741 gaggccgttg cggctatcct cgacgacgac gcagtccggg ctgtccaagc cgagccgctg
     1801 tttcgcgaga acgtaaatct cggggtccgg cttcttcttc tgcacgatgt cgccggcaag
     1861 cacgatctcg aaccaagact tgcgtttggc acccaataga ttgagcacgc cgtcgatcga
     1921 cttcggatcg gaagtcgtac agacgccgag tctgatgccc gccgcgtgcg cctcgtcgac
     1981 gatgcgcgcg atgccggggc gcagcggcag gctgccgccg gcgatcaacg ccgtgaagat
     2041 cgccgtcttc ttcttgtgca gctccggtat cagcgtctcg gccgtcttgc cgggcggcca
     2101 acccttgatg tcgaagtagt gcttcatgcg ctctttgccg ccggcgatct tcaacagctc
     2161 gccgtacagc ttgacgtccc actcggcgcg gatgccggct tcggcgaacg cacgattgaa
     2221 agcgacgcga tggccgtcgc gctcggtgtc gacgagcaca ccgtcgcagt cgaagatcaa
     2281 agccttcatg gaccgagcgt cgacgtgcgg ctcaggcgac cttcgacagc acttcgctgc
     2341 cgcgattctt gccgccgaac tgcgcgatct tttccttgaa gagctgcttc agcgcttcgt
     2401 actgcgcgcc gagcacgttg agcggctcgt atttcttgtt cttttggtgg tagtcgatga
     2461 agctgtcgat gaacacgtgc ttcaggttcg tcgagatgtt gatcttcgcg cagccgagct
     2521 ggatgcactt ctgaatgatc tcgtcgttca agcccgtgcc gccgtgcagc gccatcggcg
     2581 tgttggtgcg gcggttgatc tcgccgatca gctcgaactt cacgtcaggc tcgcctttgt
     2641 agtagccgtg cgcggtgccg atcgcggccg cgaacgccgc gaggtcgagc tcgcggcaga
     2701 accgctcggc ttcgttcggg tccgtgtagt gaaccgcgtc gacaaccatg tcgtcctcga
     2761 cgccgtggat ctcgccgatc tcggcttcga cgccgatgcc ggccctcgtc gccaggtcga
     2821 cgaccttctt cgtctgctcg aggttgacgt caaacggctt gtcggaccag tcgatcatga
     2881 ccgacgtcca gcccatctcg acgcacttct tgatcatctc gacgtctttg ccgtgatcga
     2941 gatgcagcac gacgggcacg ggcgagtcgt cggcaatctc gcgcatccaa ccgatcagag
     3001 ccttgtggcc gtagtacttg atcgtcttgg ccgagctttg aatgatgacg ggtgcgttga
     3061 gctcttcggc cgctttgacc atcgctttca tcgacgcgta gtcgacggga ttgaacgccg
     3121 ctaccgcata acccttcgct tttgcctctc gcaagacggg tttcaagttc accaacggca
     3181 tggcgttctc ctcgaaatca ggcagcggta tagctcggta atatacgtca ggcctcgaac
     3241 catgtcgccc atgagactgg cgggccgaag gcgccggagc ctcattacac cgcgcgcttt
     3301 cttaacgccc gccgccctgc ccgcggcccg gcgggcgcga gccgcgccgt tgattgcggc
     3361 cgccgtgacg atgcccttcg tgacgcggtt gcgagccgcg attgccgcga cgctcgttgt
     3421 tgcgcccggg ttgctgacgg tgctcgtggt ggcgctgcgg acgctcgtcg gcgcgcggcg
     3481 gcccaggcac gtagccttgc ggcgtgagcg tcggcagcga gtagccgagc aaacgctcga
     3541 tctgtttcag cagcggccgc tcgtcgtgcg ccacgagcga gattgcgaga ccttcgttgc
     3601 cggcacgacc cgtgcgcccg atgcgatgga cgtac
//
LOCUS       DATLEE010000664         4839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10927396, whole genome shotgun sequence.
ACCESSION   DATLEE010000664 DATLEE010000000
VERSION     DATLEE010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10927396"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VM692_14560"
     CDS             complement(<1..601)
                     /locus_tag="VM692_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HUQ53445.1"
                     /translation="MTTEHDYDALLVVSFGGPEGPADVDSFLDNVFRGLRVPPETKAH
                     IAKRYEQVGGVSPINAHTRAFIKALQLELDAHGPALPIYWGNRNWHPLLSDTVAQMAH
                     AGVKRALAFVTSTFSSYSGCRRYREDLFEAARSIPNAPLFDKLRVPYNHPGFIEAMSD
                     RVIEALDTLPAEKRTSALVLFTAHSLPLTMARGCDYEAQL"
     gene            complement(598..1329)
                     /locus_tag="VM692_14565"
     CDS             complement(598..1329)
                     /locus_tag="VM692_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HUQ53446.1"
                     /translation="MSQTTATDAAAADDTKPLDLAEKGRGAVLDRRLFMKFTAFGGCA
                     DTAAASAALAEDGVDGALYVDANDPQGIGVLVAAEDPDYFVTTLRALFNREPFASYTH
                     KPEYDMLGRTYSIGYEPDLVDTLFTKPRSKILNPAHRWAVWYPLQRAKKFETLPPDHQ
                     RRIFAEHASLGKRYGAGGHANDVRLACHGLDKNDNDFIVGLLGPNLHPLSAVVQEMRR
                     TEQTSQYLDSLGPFFVGRAVWQAKP"
     gene            complement(1326..2351)
                     /gene="hemE"
                     /locus_tag="VM692_14570"
     CDS             complement(1326..2351)
                     /gene="hemE"
                     /locus_tag="VM692_14570"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HUQ53447.1"
                     /translation="MPNDNDKLLLKAIRREAVPRRPLWIMRQAGRYLPEYREFRVKHS
                     FKELAGEAELAAEVTLMPLKRYPLDAAIVFADIMSPMPALGIEFDFDPGPVVANPIRD
                     AAAIDRLRRPAVEEIAPEVIQALKLTRAALPPSAALLGFCGAPWTLAAYLVEGKGAKD
                     FPTLRAFAAAQPALLDKLLGRLSNLMGEYLLQQAAAGADAVQIFDSWAGLLSRQSWER
                     LIRPHLTTLLEAVGKAGVPRILFLQNAPHLMDAYASLPAEVLGVDWRIDLPDLKRRYP
                     QRAVQGNLEPAILTAGAEATRTAARELLAQTPARGHIVNLGHGILPQTPLDSVAALVD
                     VVHSEEL"
     gene            complement(2361..3077)
                     /locus_tag="VM692_14575"
     CDS             complement(2361..3077)
                     /locus_tag="VM692_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUQ53448.1"
                     /translation="MKTPAHILVVDDEAHLATGIRENLEAEGYRAEVAHDGAAGLQRL
                     RAEPFDLVVLDVMMPNMDGLELCSQLRRDGIQTPVLFLTVKGAAEDRVRGFEAGGDDY
                     LAKPFHLKELLLRVAAILRRSSWYQSSASAALQFGGNRIDFKTYEARAWDGSQHSLTH
                     KEAMILRVLADQPGNIVTREEVLDRVWGYEVFPSTRTIDNFIVRLRKRFERNPEAPAH
                     FHTVRGVGYRFTAEPTEQSD"
     gene            complement(3074..4036)
                     /locus_tag="VM692_14580"
     CDS             complement(3074..4036)
                     /locus_tag="VM692_14580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUQ53449.1"
                     /translation="MTVRRDPTKAMQIGFLALLLISAAQVGWWMYDQVRYARSVEERF
                     ASSDPVVAEDSASRINRLLWEGGFFFVVLIGGMAVLTRTLRHDAELRRRQQNFLAAVS
                     HEFKSPLASIQLAAETLVLRSRDDDSKRLGTRILEDGERLLRMIDNLLDTTRLEEGRQ
                     RLTPQLTSLGASSQLAIAAIRERARASGVTVSLAVADDLVVHVDPVVVETGLRNLLDN
                     ALKSCVAAKSTSITVRGTREAGSVTLAVSDDGLGFPPEDAAMIFEKFHRLGDELRRTT
                     PGTGLGLYIVKRLVELSGGTVHAASDGPGRGATVSMRWPAPEAR"
     gene            complement(4057..>4839)
                     /locus_tag="VM692_14585"
     CDS             complement(4057..>4839)
                     /locus_tag="VM692_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUQ53450.1"
                     /translation="LAALTRVLPLDDAAAAEKLFAAEGDRIAAVIIEPVPANNGLLLQ
                     RPEFLKLLRSLCTKHGALLIFDEVISGFRLGPGGAAAHYAIKPDLATFGKVIGGGMPV
                     GGFAGPRKIMKHLSPEGGVYQAGTLSGNPVAMTAGLTTLRVLQKEDGWKRLEALGAYL
                     EQTLTNALAESPVPARLVRLGSLFWIAWHATEAPRSAEALDANAAKVYARVFHSLLDQ
                     GIALAPSAFEIAFLSLAHTRRDIDRLAEGLRAALSAVAAESD"
BASE COUNT          773 a         1598 c         1655 g          813 t
ORIGIN      
        1 cgagctgcgc ctcgtagtcg cagccgcgcg ccatcgtcaa cggtaaactg tgcgccgtga
       61 acaacacgag cgcagaggtg cgcttttccg ccggcagtgt gtcgagcgcc tcgatcacgc
      121 ggtcgctcat ggcttcgatg aaacccgggt ggttgtacgg cactcgcaac ttgtcgaaca
      181 gcggcgcgtt cggaatgctg cgcgcagcct cgaacaggtc ttcgcggtag cggcggcagc
      241 cgctgtagga gctgaacgtg ctcgtcacga acgcgagcgc gcgcttgacg ccggcgtgcg
      301 ccatctgcgc gacggtatcc gacagcaacg ggtgccagtt gcggttgccc caatagatcg
      361 gcagagcggg gccgtgcgcg tcgagctcga gctgcagcgc cttgatgaac gcgcgcgtgt
      421 gggcgttgat cgggctgacg ccgccgactt gctcgtagcg cttcgcgatg tgcgctttcg
      481 tctcgggcgg cacgcgcaag ccgcgaaaca cgttgtcgag aaacgagtcg acatccgcgg
      541 gaccttcggg cccgccgaac gatacgacga gcagcgcgtc gtaatcgtgc tcggtcgtca
      601 cggcttggcc tgccagacgg ctcggccgac gaagaacggc ccgagactat cgaggtactg
      661 cgacgtctgc tccgtccgcc gcatctcctg cacgacggcc gacagcgggt gcaggttcgg
      721 gccgagtagc ccgacgataa agtcgttgtc gttcttgtcg aggccgtggc aggcgaggcg
      781 cacgtcgttg gcgtgaccgc cggcgccgta gcgtttgccg agcgacgcgt gctcggcaaa
      841 gatgcgccgc tgatggtcgg gcggcagcgt ctcgaacttc ttcgcgcgct gcagcggata
      901 ccaaacggcc cagcggtgcg ccgggttcaa gatcttcgag cgcggcttcg tgaacagcgt
      961 gtcgacgaga tccggctcgt agccgatcga gtacgtgcgg ccgagcatgt cgtactcggg
     1021 cttgtgcgtg tagctcgcaa acggctcgcg gttgaacagc gctcgcagcg tcgtcacgaa
     1081 gtagtcgggg tcttccgccg cgacgagcac gccgatgccc tgcgggtcgt tggcgtcgac
     1141 atacaacgcg ccgtcgacgc cgtcttcggc cagtgccgcg ctcgcggcag cggtgtccgc
     1201 gcagccgccg aacgccgtga acttcatgaa gaggcgccgg tcgagcacgg cgccgcggcc
     1261 tttttccgcg agatccagcg gcttcgtgtc gtcggccgct gctgcatcgg tagcggttgt
     1321 ctggctcata gctcctccga atgcacgacg tcgacaagcg ccgcgacgct gtcgagcgga
     1381 gtctgcggca agatgccgtg ccccaagttg acgatgtgac cgcgcgccgg cgtttgcgcc
     1441 agcagctcgc gtgcggccgt ccgcgtcgcc tcggctccag ccgtcaggat cgcaggctcg
     1501 aggttgcctt gcacggcccg ttgcgggtag cggcgcttga gatccggcag atcgatgcgc
     1561 caatcgacgc cgagcacctc ggcggggagg ctcgcgtagg cgtccatgag gtgcggcgcg
     1621 ttctgcagga acaagatgcg cggcacgccg gccttgccga cggcttcgag cagcgtggtc
     1681 agatgcgggc gaatcagcct ttcccagctc tgccgcgaca ggaggccggc ccatgaatcg
     1741 aagatctgca cggcgtcggc gcctgcggcg gcttgttgca gcaagtactc gcccatgaga
     1801 ttcgagagcc ggccgagcag cttgtcgagc agcgcgggtt gtgcggctgc gaacgcgcgc
     1861 agcgtcggga agtccttcgc acctttgccc tcgacgaggt aggcagcgag cgtccacggt
     1921 gcgccgcaga atcccagcaa tgcagcgctt ggaggcagcg ccgcgcgcgt gagcttgaga
     1981 gcctgaatga cctcgggcgc gatctcctcg acggcgggcc ggcgcagccg gtcgatcgcg
     2041 gccgcgtcgc gaatcgggtt cgcgacgacg ggcccgggat cgaagtcgaa ctcgatgccg
     2101 agcgccggca tcgggctcat gatgtcggca aagacaatgg cagcgtcgag cggataacgc
     2161 ttcagcggca tcagcgtgac ctcggccgcg agctccgctt cgccggcgag ctccttgaag
     2221 ctgtgcttca cgcgaaactc gcggtactcg ggcagatagc gtccggcctg ccgcatgatc
     2281 catagtggcc ggcgcggcac cgcctcgcgg cggatcgcct tcagcaataa cttgtcgttg
     2341 tcgttcggca tccgttccct ttagtcgctc tgctcggtcg gctcggcggt gaagcgatag
     2401 ccgacgccgc gcaccgtgtg aaaatgcgcc ggcgcttcgg ggttgcgctc gaagcgtttg
     2461 cgtaggcgga cgatgaagtt gtcgatcgtg cgcgtggacg gaaacacttc gtaaccccag
     2521 acccggtcga ggacctcctc gcgcgtcacg atgttgccgg gctgatctgc gagcacgcgc
     2581 aggatcatcg cttccttgtg cgtcagcgaa tgttgcgagc cgtcccaggc gcgcgcctca
     2641 taggtcttga agtcgatgcg attgccgccg aattgcagcg ccgccgacgc gctcgattga
     2701 taccacgagc tgcggcgcag gatcgccgcg acgcgcagca gcagctcctt caagtgaaac
     2761 ggcttcgcga gatagtcgtc gccaccggcc tcgaaaccgc gcacgcggtc ctcggcagcg
     2821 cctttgacgg tcaggaacag cacgggcgtc tgaatgccgt cgcggcgcaa ttgcgagcac
     2881 agctcgaggc cgtccatgtt cggcatcatg acgtcgagca cgacgaggtc gaacggctcg
     2941 gcgcgtagac gctggaggcc tgccgcgccg tcgtgcgcga cctcggcccg atagccctcg
     3001 gcttcgaggt tctcgcggat gccggtcgcg agatgcgcct cgtcgtcgac gacgagaatg
     3061 tgcgccggcg tcttcatcga gcctccggcg ccggccagcg catggagacc gtagcgccgc
     3121 gccctgggcc gtcgctcgcg gcgtgcacgg tgccgcccga cagctcgacg aggcgcttca
     3181 cgatgtagag cccgagcccc gtccccggcg tcgtgcgccg caactcgtcg ccgagccgat
     3241 gaaacttctc gaagatcatc gcagcgtcct cgggcgggaa gccgagcccg tcgtcgctga
     3301 cggcgagcgt gacgctgccg gcctcgcgag tccctcggac ggtgatcgaa gtgctcttcg
     3361 cggcaacaca agatttcagc gcgttgtcga gcaagttgcg gagcccagtc tcgacgacga
     3421 cgggatcgac gtgcacgacg aggtcgtcgg cgacggccag cgagaccgtg acgccgctcg
     3481 cgcgcgcgcg ctcgcggatc gccgcgatcg cgagctggct cgacgcgccc aagctcgtga
     3541 gctgcggagt cagccgttgg cggccttctt cgagccgtgt cgtgtcgagc aggttgtcga
     3601 tcatgcgcaa gaggcgctcg ccgtcctcga ggatgcgtgt tccgagccgt ttgctgtcgt
     3661 cgtcgcgcga tcgcagcacg agcgtctcgg ccgcaagctg aatgctcgcg agcgggctct
     3721 tgaactcgtg cgatacggcg gccaggaaat tctgctgtct gcgccgcagc tccgcgtcgt
     3781 ggcgcagcgt gcgcgtcagc acggccatgc cgccgatcag cacgacgaag aaaaagccgc
     3841 cttcccagag caggcggttg atgcgggatg cgctgtcctc tgcgacgacg ggatcgctgc
     3901 tcgcgaaccg ctcctcgacg ctgcgcgcgt agcgcacctg gtcgtacatc caccagccga
     3961 cttgcgccgc cgagatcaac agcaacgcga ggaagccgat ttgcatcgct ttggtcggat
     4021 cgcgccgtac ggtcatgact cgtgctccgc ctcgcgtcag tcggactcgg cggccacggc
     4081 gctcagcgcc gcgcgcaagc cttcggccag cctatcgatg tcgcgccgcg tatgggccag
     4141 cgacaggaat gcaatctcga acgccgacgg cgcgagcgca atgccctgat ccagtaacga
     4201 gtgaaataca cgcgcataga ctttggcggc gttcgcatcg agcgcttcgg ccgagcgcgg
     4261 cgcctcggtg gcgtgccacg cgatccaaaa cagcgagccc aagcgcacga gccgcgcggg
     4321 cacgggcgac tctgcgagcg cgttcgtcaa cgtctgctcg agatacgcgc cgagcgcctc
     4381 gagccgtttc cagccgtctt ctttctgcag cacgcgcagc gtcgtgaggc ccgccgtcat
     4441 cgcgacggga tttcccgata acgtgcccgc ctgatacacc ccgccctcgg gcgacaagtg
     4501 cttcatgatt ttgcgcggtc ccgcgaagcc gccgacgggc atgccgccgc cgatgacttt
     4561 gccgaacgtc gcgaggtccg gcttgatcgc gtaatgcgcg gcggcgccgc cgggtccgag
     4621 ccgaaaaccg ctgatgactt cgtcgaagat caagagcgcg ccgtgcttcg tgcagagcga
     4681 gcgcagtagc ttcaggaact ccggccgctg cagcagtaag ccgttattgg ccgggacagg
     4741 ctcgatgatg acggccgcga ttcggtcgcc ttcggccgcg aacagcttct cggccgcggc
     4801 cgcgtcgtcg agcggcagca cgcgcgtcag tgccgcgag
//
LOCUS       DATLEE010000665         4195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3496075, whole genome shotgun sequence.
ACCESSION   DATLEE010000665 DATLEE010000000
VERSION     DATLEE010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3496075"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VM692_14590"
     CDS             complement(<1..311)
                     /locus_tag="VM692_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HUQ53451.1"
                     /translation="MRRLANGAAALTALAGALCGAAQAQPPDIVLLDGKIVTVDSQTT
                     VREALAIRDGKIAAVGTTAEIRGLVGPQTRVVELDGRTVIPGLIDSHLHAVRAALSFS
                     T"
     gene            443..3427
                     /locus_tag="VM692_14595"
     CDS             443..3427
                     /locus_tag="VM692_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ53452.1"
                     /translation="MPKRNCSTLASAVSSICSRRRRVTIDDLGLAAIAAVSLWGTAAN
                     AQNPTPSADAPEEITITGSRLRRDIGMSTPTPTTALDIGEMRAMAPTLLMDSLDQLPQ
                     FRDNALSQSGSIFTSGGGSQAVNLRGIGTNRTLTLLNGRRLVPGQQSGTPDVAILPTA
                     LIQRVEVVTGGASAAYGSDAISGVTNFILDTNFEGAKANLNTGMTSRHDHQNWQAEFA
                     AGSAIGEKGHIIGSVDYFDADGVIGYEGRDWGADGWAMFTRATNPLRFYAPNGRSRVI
                     TSGGLIPSGPLAFTQFVDGDPVPLGNGQVIGTAMIGGGGPDLNQEWANLRPDDKRHSA
                     FLHYKHDFSDTKTGYIQLMQGVHENTTLPSPTGMAPGWGMTIFAENPYIPASLRTRMG
                     TMASFPFNRVFEDLSPSRSIEDTATTMTIGFDGEIGNDLFLSAYVQQGKNEEHADYSE
                     NGLLVRTDRLYRALDSVIDPATGRIVCRANLFITPAQEAQISKINVQGKRVWADPESN
                     RGCIPFDPFATHLPREAIDYVTGGIHHDQELTQDVLEATLQTDIGEDRRQGSISVGGG
                     VSYREEKLFQEAFGNAGDPRRLQDFGVFNEVDGVPIRGLPGFVRDRGVFVTGNPNSQG
                     PIEGAFDVWEVFGESIIPIIGDRGGDSGVELHVAARYADYAGSGGVWASKLGADWRIN
                     PAVRFRATVSRDTRAGTLSERFDTQGAGANIALGQDPLLPTQTYIAGLTTGGNPEIRP
                     ELADTTTMGVVWQPEWADNFSFSADLYNIEIQDAIAQLGTQEILDRCYLQGAQEICAL
                     ISRNDTGTPFIRQILNVFINIAETKTSGVDLETSWRKDMRIFGGGDEEVSVRFFANYL
                     DEVSSQFVGVAALNEAGEIGNPQWLATGTFAYTNGPFRFSWQTRYRDATIREVLWRQG
                     IEIEDNSVSGRTYTNLNLSYDLEWGDASGQAYFYVGNLFDKDPPLVPGAVGATTGTAT
                     YTDNNRFDTLGRVYSVGVNFQF"
     gene            complement(3424..4038)
                     /locus_tag="VM692_14600"
     CDS             complement(3424..4038)
                     /locus_tag="VM692_14600"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HUQ53453.1"
                     /translation="MITIRPTELTGYGVRLEPLALRHERELAVAAADGSLWELWFTAV
                     PAPGETKTYIELALQRQAEGRMLPWAVRNLASGAVIGSTRFHDVVAEIDRVEIGYTWY
                     AKSWQRTHVNTTCKLLLMTHAFEQLGCKVVGLRTDKFNFASQRAIEALGARRDGLIRH
                     FSTRRDGSARDVVIYSLLASEWPEVKLHLQRRLARHGTPGGSPA"
BASE COUNT          762 a         1388 c         1371 g          674 t
ORIGIN      
        1 tcggtgctga acgacagcgc ggcacgcacg gcgtgcagat gcgagtcgat gagccccggg
       61 atcaccgtgc gcccgtcgag ctcgacgacg cgcgtctgtg ggcccacgag cccgcgaatc
      121 tcggccgtgg tgccgacggc cgcaatcttg ccgtcgcgaa tcgccaacgc ttcgcgcacc
      181 gtcgtttgtg agtccacagt cacgatcttg ccgtcgagca gcacgatgtc cggcggctgg
      241 gcttgcgccg caccgcaaag cgcgccggcg agcgccgtca gagctgcagc accgttcgcg
      301 agtcgtcgca tgtcgagccc cctttttgcg cctcgatcat agccgagcgg gcgcggcgca
      361 cgaaccgttc ggtctcggga gcttccgcaa ggtcggttac catcgcgctc gcaactatgt
      421 tggtctcgaa cccgggggaa tcatgccgaa aagaaactgt tcgacgttgg cttcggccgt
      481 cagctccatt tgctcgcggc ggcgccgagt aaccatcgac gatctcgggc tggcggccat
      541 cgccgccgtc tcgctgtggg ggaccgcggc caacgcgcaa aacccgacac cgagcgcgga
      601 cgcccccgaa gaaatcacga tcacgggctc acggctgcgc cgcgacatcg gcatgtcgac
      661 cccgactccg acgacggcgc tcgacatcgg cgaaatgcgc gcgatggcgc cgaccttgct
      721 gatggactcg ctcgatcagt tgccgcaatt ccgcgataac gcgctgtcgc agagcggcag
      781 catcttcacg agcggcggcg gctcgcaagc cgtcaacctg cgcggcatcg gcacgaaccg
      841 gaccctgacg ctgctgaacg gccggcggtt ggtgcccggc cagcagagcg gtacacccga
      901 cgtcgcgatc ctgcccacgg cgctgatcca gcgcgtggaa gtcgtcaccg gcggcgcgtc
      961 cgccgcctac ggctcggacg ccatcagcgg cgtcacgaac ttcatcctcg atacgaactt
     1021 cgagggcgca aaggccaacc tgaacaccgg catgacgagc cggcacgacc accaaaactg
     1081 gcaggccgaa ttcgcggcgg gctcggcgat cggcgaaaag ggacacatca tcggctccgt
     1141 cgactatttc gatgccgatg gcgtcatcgg ctacgaaggg cgcgactggg gcgccgacgg
     1201 ctgggcgatg ttcacgcggg cgacgaatcc gctgcgcttc tatgccccga acggccgctc
     1261 gcgcgtgatc acgtctggcg ggttgatccc gtcgggtccg ctcgccttca cgcagttcgt
     1321 cgatggcgat cccgtgcccc tcggcaacgg ccaggtcatc ggcacggcga tgatcggcgg
     1381 cggcggccct gacttgaacc aggaatgggc gaatctccgg ccggacgaca agcgacacag
     1441 cgcgttcctg cactacaagc acgacttcag cgataccaag accggctaca tccagctcat
     1501 gcagggcgtc cacgagaaca cgacgttgcc gtcgcccacg ggcatggcgc ccggctgggg
     1561 catgacgatc ttcgcggaga acccttacat cccggcgtcg ctgcggaccc gcatgggaac
     1621 gatggcgagc ttcccgttca accgggtgtt cgaggatctc tcgccgtcgc gctccatcga
     1681 agacacggcg acgacgatga ccatcggctt cgacggtgaa atcggcaacg acctcttctt
     1741 gagcgcctac gtgcaacaag ggaagaacga ggagcatgcc gactactccg aaaatggcct
     1801 gctggttcgc acggaccgct tgtatcgcgc actcgactcc gtgatcgatc ccgcgaccgg
     1861 ccgcatcgtg tgccgcgcga atctgttcat tacgccggcg caggaagcgc agatctcgaa
     1921 gatcaacgtg caaggtaagc gcgtgtgggc cgaccccgag agcaaccgcg gctgcattcc
     1981 gttcgatccg ttcgcgactc acctgccgcg cgaggccatc gactacgtca cgggcggcat
     2041 acaccacgat caggagctga cgcaggacgt cctcgaggcg acgctgcaga cggacatcgg
     2101 cgaggaccgg cgccaaggct cgatctcggt cggcggtggc gtcagctacc gcgaggaaaa
     2161 gctcttccag gaagcgttcg gcaacgccgg cgatccgcgc cggctgcaag acttcggcgt
     2221 gttcaacgag gtcgacggtg tgccgatccg cggcttgccc ggcttcgttc gcgatcgagg
     2281 cgtcttcgtc acaggcaacc cgaacagtca gggcccgatc gaaggcgcgt tcgacgtctg
     2341 ggaggtgttc ggcgagtcga tcatcccgat catcggcgac cgcggcggcg acagcggcgt
     2401 ggagttgcat gtagctgcac gctacgcgga ttacgccggc agcggcggcg tctgggcgag
     2461 caagctcggt gcggactggc gcatcaaccc ggcggtgcgg ttccgtgcga ccgtatcgcg
     2521 cgacacgcgc gccggcacgc tatcggagcg gttcgacacg cagggcgccg gcgccaacat
     2581 cgcgctcggt caggatcccc tgttgccaac gcaaacctac atcgcaggct tgacgacggg
     2641 cggcaatccc gagattcggc cggagctcgc cgataccacg acgatgggcg tcgtttggca
     2701 gcccgaatgg gcggataact tcagcttctc ggccgacctg tacaacatcg agattcaaga
     2761 cgcgatcgca cagctcggta cgcaggagat cctcgaccgc tgctacttac agggcgcaca
     2821 ggagatctgc gcgttgatct cgcgcaacga cacgggcacg ccattcatcc ggcagatcct
     2881 gaacgtcttc atcaacatcg cggaaacgaa aacgagcggc gtcgacctcg agacaagctg
     2941 gcgcaaggac atgcggatct ttggcggcgg cgacgaggag gtctcggttc gcttcttcgc
     3001 gaactacctg gacgaggtgt cgtcgcagtt cgtcggcgtc gcggcgctca acgaagcggg
     3061 cgagatcggc aatccgcagt ggctggcgac gggcacgttc gcgtacacga acggcccgtt
     3121 ccgtttcagc tggcaaaccc ggtatcgcga cgcaacgatc cgcgaggtgt tgtggcgcca
     3181 aggtatcgag atcgaggaca acagcgtctc gggccgcacg tacacgaacc tgaatttatc
     3241 gtatgacctc gaatggggcg atgcgtcggg ccaggcgtac ttctacgtcg gcaacctgtt
     3301 cgacaaggac ccgccgttgg tgccgggcgc cgtcggcgcg acgacgggca cggccacgta
     3361 tacggacaac aaccggttcg acacgctcgg cagggtgtac tcggtcggag tcaacttcca
     3421 gttctaagca ggcgagccgc ctggcgtgcc gtggcgcgcc aggcggcgct gcaagtgcag
     3481 cttcacctcg ggccactcgc tcgcgaggag gctgtagatc acgacgtcgc gcgccgaccc
     3541 gtcgcggcgc gtcgagaaat gccgaatcaa gccgtcgcgt cgcgcgccga gcgcctcgat
     3601 cgcgcgctgc gacgcgaaat tgaacttatc ggtgcgtagg cccacgacct tacagccgag
     3661 ctgctcgaac gcgtgcgtca tgagtagcag cttgcaagtc gtgttgacgt gcgtgcgctg
     3721 ccagctcttc gcgtaccacg tatagccgat ctcgacgcga tcgatctccg cgacgacgtc
     3781 gtggaagcgc gtgctgccga tgacggcgcc gctcgcgagg ttgcgcacgg cccacggcaa
     3841 catgcggcct tcggcctggc gctgcaacgc gagctcgatg taggtcttcg tctcgccggg
     3901 cgccggcacg gccgtgaacc acagctccca gagcgacccg tcggccgcgg cgaccgcaag
     3961 ctcgcgctcg tggcgcaacg cgagcggctc gagccgcacg ccgtaacccg taagctcggt
     4021 cggtctgatc gtgatcatga gctttcactc atagcggtgt cggggccgga aagatttcgt
     4081 tcgtataacc gcgcagccgg tagtacacga gaccgagcca ttcgtgcgcg gcgaggtcca
     4141 ggtcctcgag gttctccgcg agcccgtagt ggaatgcgtt cgggccgcgg cggaa
//
LOCUS       DATLEE010000666         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8699765, whole genome shotgun sequence.
ACCESSION   DATLEE010000666 DATLEE010000000
VERSION     DATLEE010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8699765"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VM692_14605"
     CDS             <1..878
                     /locus_tag="VM692_14605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HUQ53454.1"
                     /translation="DFDGVELARGMSALAPRYDGFILDQWGVLHDGTRPYDGAAECLE
                     RLHAAGKRIVVLSNSGKREAENLRVMARMGFDVSVLERCVCAGEEARAAIEARADAFH
                     AALGRRCYAFTRGGDRAVLEGIGLEFVDRIEDADFLAVLGIDSPQRSLPDYEAELTAG
                     ARKDLPMVCANPDIARLTPNGLVDAQGVLAVRYEALGGRVAFHGKPYAAIYRSCLAAL
                     ECAAERVLMVGDSVDHDVLGAQRAGIASALIPGAVHGPELGVAWGETPGAEAWRAFAA
                     RASAQPDYLLAAFNW"
     gene            880..>2279
                     /gene="glpD"
                     /locus_tag="VM692_14610"
     CDS             880..>2279
                     /gene="glpD"
                     /locus_tag="VM692_14610"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HUQ53455.1"
                     /translation="MTQDAFLAHVDILIVGGGINGAGIARDAAGRGLEVALVEQGDLA
                     SATSSASSKLVHGGLRYLEHGELRLVAEALREREVLLRAAPHIVRPMRFVLPEAPGQR
                     PAWVIRAGLLAYDWLARRQTLAASKAVSLREPPYGSGLRSDYSRGWVYSDCWVDDSRL
                     VLANARSAHDAGASIMPRTAFLGAQRRDGRWRVRLRADSGEIEMSARVIVNAAGPWAG
                     RVLDLAGVPTRIRLRLVQGSHIVVPRLYDGDHAFILQNDDGRVVFVYAYEGHTLVGTT
                     DVEIGGGPDSAGMRPDEAAYLCHAANRYFEREVHPGDVLWSYCGVRTLVDDGASAASR
                     VTRDYVLDLDGAANEAPLLSVLGGKITTHRALAERAMDKLAPWFPGMRPAWTANAPLP
                     GGDAGDGGMDEYLSRLKAQYAGLPDELLRTLLRRHGTATPDVIGDASRIEDFGEHFGA
                     QLYAREVAHFRTREWAR"
BASE COUNT          331 a          762 c          820 g          366 t
ORIGIN      
        1 aagatttcga cggtgtcgag ctcgcgcgcg gcatgtccgc gctcgcgccg cgctacgacg
       61 gcttcatact cgaccagtgg ggcgtgctgc acgacggcac gcgtccttac gacggcgcag
      121 ccgaatgcct cgagcgcctg cacgccgcgg gcaagcgcat cgtcgtgctg tcgaactcgg
      181 gaaagcgcga agccgagaac ctccgggtca tggcgcgcat gggtttcgac gtgagcgtgc
      241 tcgagcgttg cgtctgtgcc ggggaggaag cgcgcgcggc gatcgaagcc cgtgccgatg
      301 cgttccacgc ggcgctcggc cggcgctgct acgccttcac gcgcggcggt gaccgtgccg
      361 tgctcgaagg tatcgggctc gagttcgtcg atcgcatcga ggatgccgat ttccttgccg
      421 tgctgggaat cgattccccg cagcgttcgc tgccggatta cgaggccgag cttaccgccg
      481 gagcgcgcaa ggacctgccg atggtgtgcg cgaatccgga tatcgcgagg ctcacgccga
      541 acggactcgt ggatgctcaa ggcgtgctcg ccgtgcgtta cgaagcgctc ggcggacgcg
      601 tcgccttcca cggcaagccg tatgccgcga tctaccggtc gtgcctggcg gcgctcgaat
      661 gcgcggccga acgggtcctg atggtcggcg attcggtcga tcacgacgtc ctcggcgcgc
      721 agcgtgccgg aatcgccagc gcgctcatcc ccggcgccgt gcatggcccg gagctcggcg
      781 tcgcatgggg cgagacgccg ggcgccgaag cgtggcgcgc gttcgcggcc cgcgcttccg
      841 cacagcccga ctacctgctc gccgcgttca actggtgatg tgacgcagga cgccttcctc
      901 gcgcacgtcg acatcctcat cgtcggcgga ggcatcaacg gcgcaggcat tgcgcgcgac
      961 gccgccgggc gcggcctcga ggtagcgctc gtcgagcaag gcgatctcgc atccgcgacg
     1021 agctcggcga gcagcaagct cgtccacggc ggcttgcgtt atctagagca cggcgagttg
     1081 aggctcgtcg cagaggcgtt gcgcgagcgc gaagtgctgc ttcgcgcggc gccccacatc
     1141 gtgcggccga tgcgcttcgt gctgccggag gcgccggggc agcggcctgc gtgggtcatc
     1201 cgcgccggct tgctcgcgta cgattggctg gcgcggcgtc agacgctggc cgcgtcgaag
     1261 gccgtgtcgc tgcgcgagcc gccgtacggc agcggcctca ggagcgatta ctcgcgcggc
     1321 tgggtctatt cggattgctg ggtcgacgat tcaaggctcg tcctcgcgaa tgcgcgatcg
     1381 gcgcacgacg cgggcgcgag catcatgccg cgcaccgcgt tcctgggcgc ccagcgccgt
     1441 gacggccgct ggcgcgtgcg gctgcgcgcc gacagcggcg agatcgagat gagcgcgcgc
     1501 gtgatcgtaa acgccgccgg tccatgggca gggcgcgtgc tcgatctcgc cggcgtcccc
     1561 acccgcatcc gactgcggct cgtgcaaggc agccacatcg tcgtgccgcg tctatacgac
     1621 ggcgatcacg cgttcattct tcagaacgac gacggccgcg tggtcttcgt ctacgcgtat
     1681 gagggacata ccctcgtcgg gaccacggac gtcgagatcg ggggcgggcc cgacagcgcg
     1741 gggatgcgac ctgacgaagc ggcgtatctc tgccatgccg cgaaccgtta cttcgagcgc
     1801 gaggtgcatc ccggcgacgt gctgtggagc tactgcggcg tgcgcacgct cgtcgacgac
     1861 ggcgcgagcg cggcctcgcg cgtgacgcgc gattacgttc tcgacctcga cggcgccgcg
     1921 aatgaagcgc cgttgctgtc cgtgctcggc ggcaagatca ccacgcatcg ggcgctcgcc
     1981 gagcgcgcca tggacaagct ggcgccgtgg tttccgggga tgcgtccggc gtggacggcg
     2041 aatgcgccgc tgccgggagg tgacgccggc gacggaggaa tggacgaata tctcagccgt
     2101 ctcaaggcgc agtatgcggg cctgccggac gagctcctcc gaacgttgtt gcgcaggcac
     2161 ggcactgcga cgcccgatgt tatcggcgac gcttcacgca tcgaagactt cggcgagcat
     2221 ttcggcgcgc agctatacgc acgcgaggtt gcgcacttcc ggacgcgcga atgggcccg
//
LOCUS       DATLEE010000667         2930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3498752, whole genome shotgun sequence.
ACCESSION   DATLEE010000667 DATLEE010000000
VERSION     DATLEE010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3498752"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /gene="atpD"
                     /locus_tag="VM692_14615"
     CDS             complement(<1..1339)
                     /gene="atpD"
                     /locus_tag="VM692_14615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HUQ53456.1"
                     /translation="MSTGTVVQVIGAVVDVEFPRDAIPKVYDALKLQDRNLTLEVQQQ
                     LGDGVVRTIAMGTSEGLRRGLTVANTGKAISVPVGPKVLGRIMNVLGEPIDDAGEIGA
                     QESWPIHRDAPSYEEQSGSTELLETGIKVIDLIMPIAKGGKVGLFGGAGVGKTVTLLE
                     LINNIAKEHGGYSVFAGVGERTREGNDFYHEMKEAGVLNKVALVFGQMNEPPGNRLRV
                     ALTGLTMGEYFRDEGKDVLMFIDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGV
                     LQERITSTKTGSITSFQAIYVPADDLTDPSPATTFAHLDATLVLSRKIAELGIYPAVD
                     PLDSTSRLLDPNVIGQEHYDAARGTQAVLQRFKELQDIIAILGMDELSEDDKLIVQRA
                     RKIRNFLSQPFSVAQAFTGIEGKYVALKETIRSFKGIVAGEYDHLPEQAFYMVG"
     gene            complement(1357..2217)
                     /gene="atpG"
                     /locus_tag="VM692_14620"
     CDS             complement(1357..2217)
                     /gene="atpG"
                     /locus_tag="VM692_14620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HUQ53457.1"
                     /translation="MASGKEIRTKIASVKSTQKITKAMEMVAASKLRRTQQRMAASRP
                     YAEKIKTVIGHLSQANPDYRHPYLQERDIKRVGYIVISTDRGLAGGVNVNTFREMLKE
                     LREWQGKGVEADLCLVGAKGIAYFRRLKVNVVAATSHLGEQPKVQDLVGAIAAMTDAY
                     RQGKIDRLFLVHSVFVNTMSQVPTVSQLLPVEAVDKDALQQHWDYIYEPDAASLLDGV
                     LDRYIESQVYRGAVENVACEMASKMVAMKAATDNAGKFIEGLQLQYNKARQAAITREI
                     AEIVGGAAAV"
     gene            complement(2380..>2930)
                     /locus_tag="VM692_14625"
     CDS             complement(2380..>2930)
                     /locus_tag="VM692_14625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508576.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HUQ53458.1"
                     /translation="TQAGDVSAFVPTNVISITDGQIYLETDLFNAGIRPAINAGLSVS
                     RVGGSAQTKIIRKLGGGVRLALAQYRELAAFAQFASDLDAATRKQLERGRRVTELMKQ
                     KQYQPLSVAEMALTLYAVNEGFIDDVPVNKVVDFESGLHAFARGNHKPTLDAINAKPE
                     YSDANVAEMKKILESFKKTGTY"
BASE COUNT          441 a          932 c          949 g          608 t
ORIGIN      
        1 cgccgaccat gtagaacgcc tgctcgggca ggtggtcgta ctcgccggcc acgatgcctt
       61 tgaagctgcg gatcgtctcc ttcagcgcca cgtacttgcc ttcgataccc gtgaacgcct
      121 gtgcgaccga gaacggctgc gacaaaaagt tacggatctt gcgagcgcgc tgcacgatga
      181 gcttgtcgtc ttcgctgagc tcgtccatgc cgagaatcgc gatgatgtcc tgcagctcct
      241 tgaagcgctg tagcacggcc tgcgttccgc gcgccgcgtc gtagtgctct tggccgatga
      301 cgttcgggtc gagcaagcgg ctcgtcgagt cgagcgggtc gacggcgggg tagatgccga
      361 gctcggcgat cttgcgcgac agcacgagcg tcgcgtccaa gtgcgcgaac gtcgtggcgg
      421 gcgacgggtc cgtcaagtcg tcggccggga cgtagatggc ctggaacgac gtgatcgagc
      481 ccgtcttcgt cgacgtgatg cgctcttgca gcacgcccat ctcttcggcc agggtcggct
      541 ggtaaccgac ggcgctcggc atacggccca gcaatgccga tacctcggtg ccggccagcg
      601 tgtagcggta gatgttgtcg atgaacatca gcacgtcttt gccttcgtcg cggaagtact
      661 cgcccatcgt caggcccgtc agcgcgacgc gcaggcggtt gcccggcggc tcgttcatct
      721 ggccgaacac gagcgccacc ttgttgagca cgcccgcttc tttcatctcg tggtagaagt
      781 cgttgccttc gcgcgttcgc tcgccgacgc cggcgaacac ggagtagccg ccgtgctcct
      841 tcgcgatgtt gttgatgagc tcgagcagcg tgaccgtctt gccgacgccg gcgccgccga
      901 acaggccgac cttaccgccc ttcgcgatcg gcatgatcag gtcgatgact ttgataccgg
      961 tctcgagcag ctccgtcgac ccggattgtt cctcgtacga cggcgcgtcg cggtggatgg
     1021 gccacgactc ctgcgcgccg atttcacccg cgtcgtcgat cggctcgccg agcacgttca
     1081 tgatgcgccc gagcaccttg gggccgacgg gcaccgagat cgccttgccg gtgttcgcca
     1141 cggtcaaacc gcgacgcagg ccttcggacg tgcccatcgc gatcgtgcgg acgacaccgt
     1201 cgccgagttg ctgctgcact tcgagtgtca ggttgcggtc ttgcagcttc aatgcgtcgt
     1261 agaccttcgg gatcgcgtcg cgcggaaact cgacgtcgac gacggcgcca atgacttgaa
     1321 cgactgtgcc ggtgctcatg gttgcttctc tcttgattaa acggccgccg cgccgccgac
     1381 gatctccgcg atctcacgcg taatcgccgc ctggcgggct ttgttgtact gcagctgcag
     1441 gccttcgatg aacttgcctg cgttgtcggt cgcggctttc atcgcgacca tcttcgacgc
     1501 catctcgcac gccacgttct cgacggcgcc gcgataaacc tgcgactcga tgtagcgatc
     1561 gagcacgccg tcgagcagcg acgccgcatc cggctcgtag atgtaatccc aatgctgctg
     1621 caaggcatcc ttgtcgacgg cttcgacggg caggagctgc gagaccgtcg gcacctggct
     1681 catcgtgttg acgaacacgc tgtgcacgag aaacagccgg tcgatcttgc cttggcggta
     1741 cgcgtcggtc atggccgcga ttgcgccgac cagatcctgc accttcggct gctcgcccaa
     1801 gtggctcgtg gccgcgacga cgttcacctt caggcggcgg aagtaggcga tgcccttggc
     1861 gccgacgagg cacagatccg cctcgacgcc tttgccctgc cactcgcgca gctctttgag
     1921 catctcgcgg aacgtgttga cgttgacgcc gccggcgagg ccgcgatcgg tgctgatgac
     1981 gatgtagccg acgcgcttga tgtcgcgctc ttgcaggtac ggatggcggt agtcggggtt
     2041 cgcctgcgag aggtggccga tgacggtttt gatcttctcg gcgtaggggc gtgaggcggc
     2101 catgcgctgt tgcgttctgc gcagcttcga ggcggcgacc atttccatcg ctttggtgat
     2161 cttttgcgtg cttttgacgc tggcgatctt cgttcggatt tctttgccgc tggccatttc
     2221 gtttagctca tcgcgcgcgg cccgaagccg cacacaggta ttgccgacac acgccggaag
     2281 accgtccctg gggctcgtcg gcgcacatcc atgtgcgccg acggtctcgg caacacctgt
     2341 gtgcgtcttc ggaccgcgtg ggggctcatc ggtcgttcct tagtacgtgc cggtcttttt
     2401 gaaggactcg aggatctttt tcatttcggc gacgttcgcg tcgctgtact cgggtttggc
     2461 gttgatggcg tcgagcgtcg gcttgtggtt gccgcgcgcg aaggcgtgca gccccgactc
     2521 gaagtcgacg accttgttga cggggacgtc gtcgatgaaa ccttcgttga cggcgtacag
     2581 cgtcaacgcc atctcggcga ccgacagcgg ctggtactgc ttctgcttca tgagctcggt
     2641 gacgcggcgg ccgcgctcga gctgcttgcg cgtcgcggcg tccaagtccg aggcgaactg
     2701 cgcgaacgcg gcgagctcgc ggtactgagc gagcgccaag cgcacgccgc cgccgagctt
     2761 gcggatgatc ttcgtctgtg ccgagccgcc gacgcgcgac accgagagac ccgcgttgat
     2821 ggcgggccgg atgccggcgt taaacaagtc cgtctcgagg tagatctggc cgtccgtgat
     2881 cgagatgacg ttcgtcggca cgaacgccga cacgtcgccg gcttgcgtct
//
LOCUS       DATLEE010000668         4231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10125321, whole genome shotgun sequence.
ACCESSION   DATLEE010000668 DATLEE010000000
VERSION     DATLEE010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10125321"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..953)
                     /locus_tag="VM692_14630"
     CDS             complement(111..953)
                     /locus_tag="VM692_14630"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018874292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HUQ53459.1"
                     /translation="MSTPPSETDQALAGKVVAVPESRQLDVLANLLERRGAAVLRCPL
                     VGIVDSPDEPGVLGWIERLIATPTDLAVFYTGEGIQRLVGVAQRAQRAEPFIAALART
                     PKLTRGPKPRRALKTLGLEPEHPAAAPTTAGMIETARRLETPLRRVALQLYNEDQDRE
                     LVEHFERRGARVDCVAPYVYARAADDERVAALIDELASGNVDAIAFTSKAQLQRLIDL
                     AAKRQLEPALRSGLARTRVAAIGPVVAADLTDAGVRVDTMPAESYSMKPLVTSLCELL
                     AGPR"
     gene            complement(970..3246)
                     /locus_tag="VM692_14635"
     CDS             complement(970..3246)
                     /locus_tag="VM692_14635"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HUQ53460.1"
                     /translation="MRIASRCLTVAYAFVLFAFQADAQPELFPRPAALEPAVKFWTRI
                     YTEVDTKSGFIHDDLRLDIVYQTVRWSDDVGARERRRRVERATESIRTILTKLASGAR
                     QNLSDDEQRVLKLFPEGTSNAEFRAAAGRLRFQLGQSDRFREGLVRSGAWKPYIHEVL
                     AKNGLPLELAALPHVESSFDPTAYSKVGAAGLWQFMRSTGVRYMRIDHIVDERRDPFF
                     ATNAAARLLGDNFSVIQSWPLALTAYNHGLASMRRAVQQQKTTDIATIVAKYQSRSFG
                     FASRNFYTAFLAALEIDSNPDRYFPNLKIDPPSDTATITVPAFMTVDTLADALNLRES
                     TLRSLNPALTDTVWSGDKYVPQGFELRVPQNTAAVAEARLDAIEPGKLFAAQRADREH
                     REQRGDTLSKIAAEYRVSLAALMRINGMTNRSVLRVGQTIMLPTDGAIAPPDATLVRE
                     EAPAPAPAAAAPALASTSAEGLYVVRRGDSIERIATRLGVDPQALVAANDIRNRNVIQ
                     VGQQLIVPTAPGAVIAVAAVTTTLPTVEPPVSAALDSPAAVAAEPVAAADLTAALAPA
                     VLANGEPAPVSEADESADDEVNVLAVEQDVLAADPSDYTVSATNEISVQPLETLGHYA
                     DWLEIPTQRLRDLNSLSFREGVVLGQTLTLEFARIDAATFEQRRRAYHQQLQSDFFAA
                     YQIEDVESHVMRSGESLWILAARTYNVPVWLLRQYNPDLNLDRIQAGVVVKFPRLKAI
                     AADTAVASRPQVLADNER"
     gene            complement(3438..4061)
                     /locus_tag="VM692_14640"
     CDS             complement(3438..4061)
                     /locus_tag="VM692_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUQ53461.1"
                     /translation="MLTIYGRTNSVNVQKVLWCLAELEVSYTRIDAGLEHGKNNEPWY
                     LALNPNGRIPLLQDGTFSLWESNTIVRYLAAKHESRELYPASPESRALAERWMDWQLS
                     TLVLPVSIVFQNLYRTPAAKRDAAAIERNLREANKAMTLLDAHLAQHAYVAGAAFTMG
                     DIPVGATAHRWLEVEGIDRPAFAAVRAWRARLAERPGFRAHVQLPLS"
BASE COUNT          644 a         1376 c         1489 g          722 t
ORIGIN      
        1 agcgccgatc cgctgaagga ttaccgcacg gcggtcgcgg acttctcggt ttggtatcag
       61 ccgacggccg acggccgccc cacggccatg cagctacgcc gggcgaagca ctaacgcggc
      121 ccggccagga gctcgcatag gctcgttacg agcggtttca tcgagtagct ctccgccggc
      181 atcgtgtcga cgcggacgcc cgcgtccgtc aagtcggcgg ccacgacggg acctatcgcg
      241 gcgaccctcg tgcgcgcgag cccggagcgc aacgccggct cgagctgccg cttggcggcg
      301 agatcgatca ggcgctgcag ttgcgctttg ctcgtgaacg cgattgcgtc gacgttgccc
      361 gaggcgagct cgtcgatcaa cgccgcaacg cgctcgtcat cggcggccct tgcgtagacg
      421 tagggcgcga cgcaatcgac tcgagcgccg cgtcgctcga agtgctcgac gagctcgcgg
      481 tcctggtcct cgttgtagag ctgcagggcg acgcgccgca gcggcgtctc gagccttcgc
      541 gcggtttcaa tcatgcccgc cgtcgtgggc gcggcggccg ggtgctcggg ctccagcccc
      601 agtgtcttca gagcgcgtct cggcttcggg ccgcgcgtga gcttcggggt ccgcgcgagc
      661 gctgcgatga acggctccgc acgctgagcg cgttgcgcga ccccgacgag ccgttggatg
      721 ccctcgcccg tgtagaagac ggcgaggtcc gtcggcgtcg cgatcagccg ctcgatccaa
      781 ccgaggacgc cgggctcgtc cggcgagtcc acgatgccga cgagcggaca acgcagaacg
      841 gcggcgccgc gccgctcgag cagattcgcg aggacgtcga gctggcgtga ctcggggacg
      901 gcgacgactt tgcccgcgag cgcttgatcg gtttcgctgg gcggcgtgct catcgcggca
      961 tggcgcgcct cagcgctcgt tgtcggcgag cacctgcggt cgggaggcga cggccgtgtc
     1021 ggcggcgatg gctttcaagc gcggaaactt gaccacgacg ccggcctgga tccgatcgag
     1081 attcaggtcg gggttgtatt ggcgcagcag ccagaccggc acgttgtagg tccgagcggc
     1141 caggatccac agcgactcgc ccgatcgcat gacgtggctc tcgacgtcct cgatctgata
     1201 ggcggcgaag aaatcgctct gcagctgttg gtggtaggcg cgtcggcgtt gctcgaacgt
     1261 cgcggcgtcg atgcgcgcga actcgagcgt cagcgtctga cccagcacga cgccctcgcg
     1321 gaaggacaac gagttcagat cgcggagccg ctgcgtcggg atctcgagcc aatccgcgta
     1381 gtggccgagc gtctcgagcg gctgcacgga gatttcgttc gttgcgctga cggtgtagtc
     1441 gcttggatcc gcggcgagca cgtcctgctc gaccgcaagc acattgacct cgtcgtcggc
     1501 cgactcgtcg gcctcgctga ccggcgccgg ctcgccgttc gcgagcacgg ccggtgcaag
     1561 cgcggccgtc aggtcggcgg ccgcgacggg ctcggcggcc accgcggccg gcgagtccaa
     1621 cgccgcgctg acgggtggct cgaccgtcgg cagcgtcgtc gtcacggccg ccacggcgat
     1681 gacggcgccc ggcgccgtcg ggacgatcag ctgctggccg acctggatga cgttgcggtt
     1741 gcgaatgtcg ttcgctgcaa ccaacgcttg cgggtcgacg ccgaggcgcg tcgcgatccg
     1801 ctcgatgctg tcgccgcgcc gcacgacgta gaggccctcg gcgctcgtcg aggccagtgc
     1861 cggcgccgca gcggccggag cgggggccgg cgcttcttcg cgcacgagcg tggcgtcggg
     1921 cggagcgatg gcgccgtcgg tcggcaacat gatcgtttgg ccgacgcgca ggacactgcg
     1981 gttcgtcatg ccgttgatcc gcatcagggc cgcaaggctc acgcggtact ccgcggcgat
     2041 cttcgacaac gtatcgccgc gttgctcgcg gtgctcgcgg tcggcgcgtt gcgcggcgaa
     2101 cagcttgccc ggctcgatgg cgtcgagccg agcttcggcg acggcggccg tgttctgggg
     2161 tacgcgcagc tcgaagcctt gcggcacgta cttgtcgccc gaccagaccg tgtcggtcaa
     2221 cgccgggttc agcgagcgca gcgtgctctc gcgcaaattc agcgcgtcgg ccagcgtgtc
     2281 gaccgtcatg aacgccggca ctgtgatcgt ggccgtgtcc gacggcgggt cgatcttcaa
     2341 attcgggaag tagcggtcgg gattcgagtc gatctcgagc gctgcgagga aggccgtgta
     2401 gaagttgcgc gatgcgaaac cgaacgagcg gctctggtac ttcgcgacga tcgtcgcgat
     2461 gtcggtcgtc ttctgctgct gcacggcgcg gcgcatgctc gcgaggccgt gattgtaagc
     2521 cgtcaacgcg agcggccacg actggatgac gctgaagttg tcgccgagca agcgagcggc
     2581 ggcattcgtc gcgaagaacg ggtcgcgccg ctcgtcgacg atgtgatcga tgcgcatgta
     2641 gcgcacgccc gtcgagcgca tgaactgcca gaggcccgcg gcgcctacct tcgagtaggc
     2701 ggtcgggtcg aacgacgact cgacgtgcgg caacgcggcg agctcgagcg gcaagccgtt
     2761 cttcgcgagc acctcgtgga tatacggctt ccacgcgccg gagcgcacga gcccttcgcg
     2821 gaagcggtcg gactggccga gctggaagcg caaccggccg gcggcggcgc gaaactccgc
     2881 gttgctggtg ccctccggga acagcttcag gacgcgctgc tcgtcgtcgc tcaagttctg
     2941 gcgcgcgccg ctcgcgagct tcgtgagaat cgtgcgaatc gattccgtcg cgcgctcgac
     3001 gcggcgccgc cgctcgcgag cgccgacgtc gtcggaccag cgcacggttt gatagacgat
     3061 gtcgagacga aggtcgtcgt gaatgaagcc ggacttcgtg tcgacttcgg tgtagatgcg
     3121 cgtccaaaat ttgacggccg gctcgagcgc cgcgggccgc gggaagagct cgggttgagc
     3181 gtcagcttga aacgcgaaca agacgaaggc gtaggctacc gtgaggcagc gagatgcaat
     3241 gcgcatgtag tggcttccga ttccctgcga gctctgaggc cgttcttgga ttatgtccat
     3301 cgcgcccgcc gcccgtcccg gacgtagttc acgtccgaaa aaggctgctg ggcaattctt
     3361 cccctgcggt tttcttgttt gtttctatcg gcttacagcc ggaaattacc gcaccgcctg
     3421 ggcggcgtca aaggctttca gctcaagggt agctggacgt gggcgcggaa cccggggcgc
     3481 tcggcgagcc gcgcccgcca agcgcgcacg gcggcgaacg ccggccggtc gataccctcg
     3541 acttcgagcc agcggtgcgc cgtggcgccg acggggatat cgcccatcgt gaaggccgcg
     3601 ccggccacgt acgcgtgctg ggccagatgg gcgtccagca gcgtcatcgc cttgttcgct
     3661 tcgcgcagat tgcgctcgat cgcggccgcg tcgcgcttcg ccgccggcgt ccgataaaga
     3721 ttctggaaca cgatcgacac gggcagcact agggtcgaga gctgccagtc catccagcgc
     3781 tcggcgagag cgcgtgactc cggcgacgcc gggtacagct cacgcgactc gtgcttcgcg
     3841 gcgagatagc gcacgatcgt gttcgactcc cacaaagaga acgtgccgtc ctgcagcagc
     3901 gggatccggc cgttcggatt cagcgcgaga taccacggct cgttgttctt gccgtgctcg
     3961 agcccggcgt cgatgcgcgt gtacgaaact tcgagctccg caaggcacca cagcaccttc
     4021 tgcacgttga cggagttcgt tctgccgtag atcgtgagca cgtcagcctt cgctcgcgcg
     4081 ggatggtggt attctcgcac ggcaccgacg cgcgtgctga tgcggggcaa cgccgctgtt
     4141 acggagaacg ttcaaaattt cgttgtgggc cgtcgtgctg tttgcggctc tgtcgatcgt
     4201 gctgctgacg gcaacggtct acacctacga c
//
LOCUS       DATLEE010000669         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10126117, whole genome shotgun sequence.
ACCESSION   DATLEE010000669 DATLEE010000000
VERSION     DATLEE010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10126117"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VM692_14645"
     CDS             <1..883
                     /locus_tag="VM692_14645"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HUQ53462.1"
                     /translation="VQRVSTFSAGIAVGARSPAAARELIAFLASPALGPVVEKYGLEQ
                     IGAEGKDWRVLFNGRDLAGWTPKIRKHTLGDNYANTFRVVDGLLTVAYDGYETFDEQF
                     GHLFFEQPFSHYRLRIEYRFVGAQARDAPAWAARNSGVMVHAQPPATMLRDQDFPISI
                     EVQFLGGLSDGKPRPTGNLCSPGTRVVYAGQPDTTHCIQAAAPTIDGDGWITADVLVE
                     GSERIVHYIDGAPVIEYGGISYGGGNVNGHDPQAKPDGRPLASGYLALQSESHPIQFR
                     RVELLDLDLPVNGEARR"
     gene            880..1014
                     /locus_tag="VM692_14650"
     CDS             880..1014
                     /locus_tag="VM692_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53463.1"
                     /translation="MSVAPELEAAAIAAIASEIDDSAPARSEAACANCGTPVIPGKPS
                     "
     gene            1051..2103
                     /locus_tag="VM692_14655"
     CDS             1051..2103
                     /locus_tag="VM692_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HUQ53464.1"
                     /translation="MRFVIGLALVIALARVGSAQVEQPQTEDATEAAAFAAFLAEIRT
                     AASQRGIDDATLDAVLPSIRIHRRAIQADRSQAEFVETYETYLSRRVTPQRIDRGRAL
                     LAEQGEMIRRVTADYGVQPRFVAAIIGLESNYGTYPIEEPLFDVLATLAFDGRRGAQF
                     RTQLLAALEIVAKGYAKPEELKSSWAGALGVPQFTPTNVLEIAVDHDKDGHIDLWKIG
                     PDVIASVAHYLQRTGWRADQTWGRAVALPAGGEQSLAAAMRDGVSAPRVCRSYESHGA
                     FRGLKAWQQLGVRAADGGDLPARNLDAALIIGDEGDDRGWLVYGNFCSLMRYNPAFRY
                     ALSVGLLADALGAADR"
BASE COUNT          356 a          742 c          728 g          323 t
ORIGIN      
        1 ggtgcagcgc gtcagcacgt tctcggccgg catcgccgtc ggcgcgcgca gtcccgcagc
       61 cgcgcgcgag ctcatcgcgt tcctcgcgtc gccggcgctc ggccccgtcg tcgagaaata
      121 cgggctcgag caaatcggcg cggaaggcaa ggactggcgc gtgctgttca acggccgcga
      181 cctcgcgggt tggacgccga agattcggaa gcacacgctc ggcgacaact acgccaatac
      241 gtttcgcgtc gtcgacggcc tgttgaccgt cgcgtacgac ggctacgaga cgttcgacga
      301 gcagttcggc cacctgtttt tcgagcaacc gttttcgcac taccgcttgc gcatcgagta
      361 tcggttcgtc ggcgcgcagg cgcgcgatgc gccggcctgg gccgcacgca acagcggcgt
      421 catggttcac gcgcagccgc cggcgacgat gctgcgcgac caggactttc cgatctcgat
      481 cgaggtgcaa ttcttgggtg gcttgagcga cggcaagccg cgaccgacgg gcaacctctg
      541 ctcgccgggg acccgagtcg tctacgccgg gcagcccgat acgacgcatt gcatccaggc
      601 ggcggcgccg acgatcgacg gcgacggctg gatcacggcc gacgtgctcg tcgaaggcag
      661 cgagcggatc gtgcactaca tcgacggcgc gcccgtcatc gagtacggcg gcatcagcta
      721 cggcggcggc aacgtcaacg gccatgatcc gcaagcgaag cccgacggca ggccgctcgc
      781 gagcggctat ctcgcgctgc agagcgagag tcacccgata caatttcggc gcgtcgagct
      841 gctcgacctc gatctgcccg tgaacggcga ggcgcggcga tgagcgtcgc gccggagctc
      901 gaggccgcgg cgatcgcggc catcgcgagc gagatcgacg actccgcgcc ggcgcgaagc
      961 gaagccgcgt gcgcgaattg cggcactccg gtcattcccg ggaaaccgtc ctagccgacg
     1021 tcatcaaggc tgcgcagcga gggaacgctc atgagattcg tcattggact ggcattggtc
     1081 atcgcgctag ctcgcgtcgg cagcgcgcag gtcgagcagc cgcagaccga ggacgcgacg
     1141 gaagcggcgg cattcgcggc gttcttggcc gaaattcgca ccgcggcgtc gcagcgcggc
     1201 atcgacgacg caacgctcga cgccgtgttg ccctcgatcc gcattcatcg ccgcgccatc
     1261 caggccgatc gctcgcaagc cgagttcgtc gagacctacg agacgtatct ctcgcgacgc
     1321 gtgacgccgc agcgcatcga ccgcggccga gcgctgctcg ccgagcaggg cgagatgatc
     1381 cggcgcgtga cggccgacta cggcgtgcag ccgcggttcg tcgcggcgat catcgggctc
     1441 gagagcaact acggcacgta tccgatcgaa gagccgttgt tcgacgtgct cgcgacgctg
     1501 gccttcgacg gccggcgcgg cgcgcaattt cgcacgcaac tgctggccgc gctcgagatc
     1561 gtcgcgaagg gctacgcgaa gcccgaagag ctcaagagct cgtgggccgg agcactcggc
     1621 gtgccgcagt tcacgccgac gaacgtgctc gagatcgccg tcgaccacga caaggatggc
     1681 cacatcgacc tctggaagat cggtcccgac gtgatcgcct ccgtcgccca ctatttgcaa
     1741 cgcacgggtt ggcgcgccga ccaaacctgg ggccgcgccg tcgcgctgcc ggccggcggc
     1801 gagcagtcgc tcgccgcagc gatgcgtgac ggcgtcagcg cgccgcgcgt ctgtcgcagc
     1861 tacgagagcc acggcgcctt ccgcggcttg aaggcctggc aacaactcgg cgtccgtgca
     1921 gccgacggcg gcgacctgcc ggcgcgcaac ctcgatgccg cgctcatcat cggcgacgaa
     1981 ggcgacgatc gcggctggct cgtctacggc aacttctgct cgctgatgcg ctacaacccc
     2041 gccttccgct acgcgctgtc agtcggcttg cttgccgacg ccctcggtgc ggcggaccga
     2101 tagaggcgtg ctatcgtcgc cgcggtggaa ctcaaggccg acatcgttg
//
LOCUS       DATLEE010000670         2392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10935251, whole genome shotgun sequence.
ACCESSION   DATLEE010000670 DATLEE010000000
VERSION     DATLEE010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10935251"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VM692_14660"
     CDS             <1..520
                     /locus_tag="VM692_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUQ53465.1"
                     /translation="LSPEAALGQPVDARTDVFALGIITWEMLTGRRLFYADDPHATIK
                     LVREARVPSMSSYNPKVDSELEEIVRRALARDPNDRTPNAADYAEDLSNHLFNRELKV
                     TSRDVTNAVKAVRAAKKGPTANQQLINALIADEIAKMTSLVDKMPAAGDAPKPVDIVN
                     TADWASELLDDK"
     gene            complement(532..1113)
                     /locus_tag="VM692_14665"
     CDS             complement(532..1113)
                     /locus_tag="VM692_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53466.1"
                     /translation="MVMLVFVSNRLRDLSAIEARLLDLAHTTDAKLTAAALAYYAPCS
                     IEDAQRVLDDLTVRGTLSMDVEDDGSIVYSMLGRQKFAARGNHAPAPLSLVPFRPAPA
                     PVRYAVPRGASPVLAALLTLFVPGAGHLYAGRIVAGIFWFMVVGLGYALFFPGLILHL
                     FAVVSSVNAASRLEERRAHALMAAQPQAYRLAA"
     gene            1208..1852
                     /locus_tag="VM692_14670"
     CDS             1208..1852
                     /locus_tag="VM692_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53467.1"
                     /translation="MKLAAVLLSLTAASACAGPDDSDMLTPDAAVAQPDAPNEPKPAF
                     SASTMISGKLAIDDTGIRETYKRQGDVKPSVVSVGLTETATTKNCGVTLAPKLVQFGS
                     ASTSTRQFKTVVLDFANSKIVEDTCGWDDAWILSQLDQQFGHYIVGFAKARFAEDQPY
                     LDVYYDAVQPFPNSTANIVSTGSGSAYGMNADGSITLTMVEPTPGTLLPALYSF"
     gene            2008..>2392
                     /locus_tag="VM692_14675"
     CDS             2008..>2392
                     /locus_tag="VM692_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53468.1"
                     /translation="MKRAALLALVVAACDPIDFEFDLDLDFGGGPITGEHGAATFSLV
                     DGCPDGNFLFGCPKTMPAFAVGARARMVIGSVSGDADHEQQLATAQFVVSDPSVLAFG
                     RDADGFVVMQALGEGTAMIEIDDKAG"
BASE COUNT          441 a          799 c          774 g          378 t
ORIGIN      
        1 tctctcgccg gaagccgcgc tcggtcagcc ggtcgacgcg cggaccgacg tgttcgcgct
       61 tggcatcatc acgtgggaga tgctgaccgg ccgccgcctg ttctacgcag acgatccgca
      121 cgccacgatc aagctcgttc gagaggcgcg cgtgccgtcg atgtcctcgt acaacccgaa
      181 ggtcgattcc gagctcgagg agatcgtgcg tcgcgcgctt gcccgcgatc ccaacgaccg
      241 cacgccgaac gccgccgact acgccgaaga tctctcgaac cacctgttca atcgcgagct
      301 caaggtcact tcgcgcgacg tcacgaacgc ggtgaaggcc gtgcgtgccg ccaagaaggg
      361 gccgacggca aatcagcagc tcatcaacgc cctcatcgcc gacgagatcg cgaagatgac
      421 gtcgctcgtc gacaagatgc cggcggctgg cgatgcgcca aagccggtcg acatcgtcaa
      481 taccgccgac tgggcgtccg agctgctgga cgacaagtga gccgcaagta actaggcggc
      541 gaggcgatac gcctgcggct gcgcggccat cagcgcgtgc gcgcggcgct cctcgagccg
      601 gctcgctgcg ttcacggacg acacgaccgc gaacagatgc aggatcaggc ccgggaagaa
      661 cagcgcgtag ccgaggccga cgaccatgaa ccagaagatg ccggcgacga tccggccagc
      721 gtagagatgg ccggcacccg gaacgaacaa cgtcagcagc gcggcgagca ccgggctcgc
      781 gccgcgtgga accgcgtagc gaaccggcgc gggggcggga cgaaacggca cgagcgagag
      841 tggcgccggc gcgtggttgc cgcgcgcagc gaacttctgc cggccgagca tcgagtagac
      901 gatcgagccg tcatcctcga cgtccatcga tagcgtcccg cgcacggtca gatcgtcgag
      961 aacgcgctgt gcatcttcga tcgagcacgg cgcgtagtag gcgagagccg ctgcggtcag
     1021 cttcgcgtcg gtggtgtgag cgagatcgag gagccgcgct tcgatcgcgc tgagatccct
     1081 gaggcggttg gacacgaaga ccaacataac cacgcggaat ccgttgtcac tccccattcg
     1141 ggcagcctga cgcgcactgg gcgtactgaa tgttcccatt cgacgccgca actgcgtcta
     1201 gcgggtcatg aaactcgccg ccgtcttgtt gtctctcact gccgccagcg cctgcgccgg
     1261 tcccgacgat tccgacatgc tcacgcccga cgcggcggtc gcgcagcccg acgcgccgaa
     1321 cgagccgaag ccggcgtttt ccgcgagcac gatgatctcc gggaagctcg cgatcgatga
     1381 cacgggcatt cgcgagacgt acaagcggca aggcgacgtg aaaccgtcgg tcgtatctgt
     1441 cggtctgacc gagacggcga cgaccaagaa ctgcggcgtg acgctggcgc ccaagctcgt
     1501 gcagttcggt tccgcgagca cctcgacgcg ccagttcaag accgtggttc tcgacttcgc
     1561 caattcgaag atcgtcgagg acacgtgcgg ctgggacgac gcgtggatcc tgagccagct
     1621 cgatcagcag ttcgggcatt acatcgtcgg cttcgcgaag gcgcggttcg ccgaggatca
     1681 gccgtacctc gacgtctact acgacgcggt gcagccgttc ccgaacagca ccgcaaacat
     1741 cgtgagcacg ggcagcggca gcgcgtacgg catgaacgcc gacggcagca tcacgctgac
     1801 gatggtcgag cctacgccgg gcacgctact tcccgcgctc tacagcttct gatctgaccg
     1861 agcggctact tctgatcgaa cgccgcgacg agtgcgagct tgcggatctg cgcttcggtg
     1921 atcactcgcc ggcatcttcg caggctttcc gttcgctgcc acatgctgcg ctacgcaggt
     1981 cggaccactc gcgggtattc ccgtcatatg aaacgcgctg cccttctcgc gctcgtcgtt
     2041 gctgcatgcg acccgatcga tttcgaattc gatctcgacc tcgacttcgg cggtgggccg
     2101 atcaccggcg agcacggcgc cgccacgttc tcactcgtcg acggctgtcc cgacggcaac
     2161 ttcctgttcg ggtgcccgaa gaccatgccg gcatttgccg tcggggcgcg agcgcgaatg
     2221 gtcatcggct cggtcagcgg cgacgccgac cacgagcagc agctcgcaac cgcgcagttc
     2281 gtcgtcagcg acccgagcgt gctcgcgttc gggcgcgatg ccgacgggtt tgtcgtcatg
     2341 caggcactcg gcgagggcac ggcgatgatc gagatcgacg acaaggccgg ca
//
LOCUS       DATLEE010000671         3943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7213609, whole genome shotgun sequence.
ACCESSION   DATLEE010000671 DATLEE010000000
VERSION     DATLEE010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7213609"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VM692_14680"
     CDS             <1..363
                     /locus_tag="VM692_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUQ53469.1"
                     /translation="AGVEQMRASMRKLRSTRIDLMQVHNLLDARAHLDTLGEWKREGR
                     VRYVGVTHYTAGAHDDVARLIGERPVDFIQINYSAAERDAERRLRFNGGYRRSDGEHE
                     RSDAPHRYSKCNEARSTA"
     gene            complement(327..1316)
                     /locus_tag="VM692_14685"
     CDS             complement(327..1316)
                     /locus_tag="VM692_14685"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ53470.1"
                     /translation="MRNVADKSLVSLVAAAAVWALAVAGALAQAEADSEVRALDQRIA
                     DAVVRGDTDYVARVTADDFVMVHGDGWTHGGQPLRTDDKQALLDRVATKYYDVLAFDS
                     VQAELHGNVAITHGRYLAHTPGVTDANRAWFSVRYERVYAQRDGGWQYLSHRTVHGPI
                     YGPSREAVVDDADQTSPPTASTTSVTVPSAAGGEVLELERAIGMAIARGHTQYFDEAT
                     ADDFVMLHGDGWTKGGQAALVDDKASFMPRVASNAYAVHDYDRQAVELHDDVAITYGR
                     YIGNIPSSPPDRRWFYVWYQKVYAQRDGRWVYLSHRTVDGAHYAVDRASLHLE"
     gene            complement(1384..1809)
                     /locus_tag="VM692_14690"
     CDS             complement(1384..1809)
                     /locus_tag="VM692_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HUQ53471.1"
                     /translation="MDTDRPSKPMTLSHYRALADFRYELRRFLRYSEETTRRHGVTPL
                     QYQVLLQVKGYPEGKEATVGGLAERLQAKHHGVVALVSRCEDARLVTRRISDQDRRAV
                     VVDLTPKGERLVERLARLHRTELMSIQDRISFPGLKALE"
     gene            1916..2533
                     /locus_tag="VM692_14695"
     CDS             1916..2533
                     /locus_tag="VM692_14695"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HUQ53472.1"
                     /translation="MNEFADELLPPQSWPAVRAWRKAKRTELLSRRKALTFHDRQAYA
                     ESLTAHLLATLELRPAGVLGIYWPINGEVDLRDYARRYVETGGTAALPVVVEKGAPVE
                     FWQWQPGASLRRGFWNIPVPVERRVVTPDTLLIPLVGHDAAGYRLGYGGGYYDRTLAA
                     LAPRPLCVGVGYDEARLATIHPQPHDIPMDWIVTERRVQRIAHEA"
     gene            complement(2535..>3943)
                     /locus_tag="VM692_14700"
     CDS             complement(2535..>3943)
                     /locus_tag="VM692_14700"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ53473.1"
                     /translation="PTIVGNTVIVPAAHTPLAPPDQAKNVVGYIRAFDVVTGKLLWTF
                     HTVPRPGEPGYETWLNDSARTGGGNAGVWATIAADEDLGLAYLPVESPYGDMYGGLRP
                     GANLYGESVVAVDLETGERRWHFQTSHHPLWDYDLPTAPILVDAVKDGRTVKALAQAT
                     KQGLLFVLNRETGEPVWPIPEVAVPQGNVPGEWYSPTQPIPTLRYGHQGITADDLIDF
                     TPALRAEAERVIANHRIGPIYTPAVPFNPNGPVSTLMVMGGSNWPGGSYDPENKIVFV
                     TASVGVNSMTICRYAEGSVMPHGVCTGPDAGAFGGLRNVSVQGLPLLKPPYGTIAAFD
                     LTKGEKLWEIPNGDTPENVRNHPALQGVDVGRTGRAGQPPGALATKTLVIVAEPGYGP
                     TPDGKRGSMLRAYDKRTGRELAALQLPAPQSGSPMTYMLDGRQFLVIAVSGTDRPGEL
                     LAYALPQGRPAAARGAGE"
BASE COUNT          617 a         1338 c         1330 g          658 t
ORIGIN      
        1 gccggcgtcg agcagatgcg tgcgtcgatg cggaagctgc gctcgacgcg catcgacctg
       61 atgcaggtgc acaacctgct cgacgcgcgg gcgcacctgg ataccctggg cgagtggaag
      121 cgcgaggggc gcgtgcgcta cgtgggcgtg acccactaca cggccggcgc gcacgacgac
      181 gtcgcccgcc tgatcggcga gcgcccggtg gacttcatcc agatcaacta ctcggccgcc
      241 gagcgcgacg ccgagcggcg gctgcgcttc aacggcgggt accgtcgcag cgacggcgag
      301 catgaacgca gtgacgcgcc tcaccgttac tccaaatgca acgaggctcg atccacggcg
      361 tagtgagcac cgtcgaccgt gcggtgcgac aagtagaccc agcgtccgtc gcgttgcgcg
      421 tagaccttct ggtaccagac gtagaaccag cggcggtccg gcggcgagct cgggatgttg
      481 ccgatgtagc ggccatacgt gatcgcaacg tcgtcgtgca gctcgacggc ctgccggtcg
      541 tagtcgtgaa cggcataggc gttgctcgcg acgcgcggca tgaagctcgc cttgtcgtcg
      601 acgagcgccg cttgcccgcc cttcgtccaa ccgtcgccgt gcagcatcac gaaatcgtcc
      661 gcggtggcct cgtcgaagta ctgcgtgtgg ccgcgtgcga tggccattcc gatcgcgcgc
      721 tcgagctcga ggacttcgcc gccggcggcc gaaggcaccg taacgctcgt cgtggaagcc
      781 gtcggcggcg aggtctggtc cgcatcgtcg acgaccgcct cacggctcgg gccgtaaatc
      841 gggccgtgca cggtgcgatg tgaaaggtac tgccagccgc cgtcgcgctg cgcatagacg
      901 cgctcgtagc ggactgagaa ccaggcgcga tttgcgtcgg taacgcccgg cgtatgcgcg
      961 aggtagcggc cgtgagtgat ggccacgttg ccgtgcagct ccgcttgcac ggagtcgaag
     1021 gcgagcacgt cgtagtactt cgtcgcgacg cgatccagca aggcctgctt gtcgtcggtc
     1081 cgcagtggct ggccgccgtg cgtccagccg tcgccgtgga ccatgacgaa gtcgtcggcc
     1141 gtcacgcgcg cgacgtagtc ggtgtcgccg cgcacgacgg cgtcggcgat gcgctgatcg
     1201 agcgcgcgca cttcggaatc ggcttcggcc tgcgccaatg cccccgcgac ggccaacgcc
     1261 caaaccgcgg cggctgcgac gaggctgacc agactcttgt cggcaacatt ccgcacgtct
     1321 tccccctcgg cattgggtcc cggcccaaat ttcagcacgt agctcgggcg ccgtccacga
     1381 gcattactcc aaggctttca gacccggaaa cgagatccga tcctggatgc tcatgagctc
     1441 cgtgcgatgc aagcgcgcga ggcgctcgac caagcgttcg cccttcggcg tgagatcgac
     1501 gacgacggcg cgtcgatcct ggtcgctgat acggcgcgtc acgaggcgtg cgtcctcgca
     1561 gcgagagacg agcgcgacga caccgtgatg cttcgcttgc agccgctcgg cgagcccgcc
     1621 gacagtcgct tctttgcctt ccggataccc tttcacctgc agcagcacct gatactgcaa
     1681 cggcgtcacg ccgtgccggc gcgtggtctc ctcgctgtaa cgcaagaacc gccgcagctc
     1741 gtagcgaaaa tctgcgagcg cgcggtagtg actcaatgtc atcggcttcg acggtcggtc
     1801 cgtatccatg ggcgacgctc gttgcggtgc tgccttttta tatcacgttg tgatgtgtta
     1861 ccgtcgagtg gtgagcgagc cagaatccca cgaaccgagc tctcccacgt gcctcatgaa
     1921 tgagttcgcg gacgagctgc tgccgccgca aagctggccg gcggtgcgcg cgtggcgcaa
     1981 ggccaagcgc acggagctcc tgtctcgccg caaagcattg acattccacg atcgccaggc
     2041 ctatgccgaa agcctgacgg ctcacctgct cgcgacgctc gagctgcgcc cggccggtgt
     2101 gctcggcatc tattggccga tcaacggcga ggtcgacctg cgcgactacg cgcggcgcta
     2161 cgtcgaaacc ggaggcacgg ccgcattacc cgtcgtcgtc gagaaaggtg cgcccgtcga
     2221 attctggcag tggcagcccg gcgcgtcgct gagacgcggc ttctggaaca tcccggtgcc
     2281 cgtcgagcgc cgcgtcgtca cacccgacac gttgctgatt ccgctcgtgg gccacgacgc
     2341 ggccggctat cgcctcggtt acggcggcgg ttactacgat cgcacgctgg ccgccctggc
     2401 gccgcgcccg ttatgtgtag gcgtcggcta cgacgaagcg aggctcgcga cgattcaccc
     2461 gcagccgcac gacatcccga tggattggat cgtgacggag cgccgcgtgc agcgcatcgc
     2521 gcacgaagcc tgagttactc gccggccccg cgtgctgcgg ccggccgccc ttgcggcaac
     2581 gcgtacgcca gcaactcgcc ggggcggtcc gtgccgctga ccgcgatgac gagaaactgc
     2641 cggccatcca gcatgtacgt catcggcgag ccgctctgcg gcgccggcag ttgcagcgcc
     2701 gcgagctcgc ggcccgtgcg cttgtcgtac gctcgcagca tcgagccgcg cttgccgtcg
     2761 ggcgtcggcc cgtagccggg ctccgcgacg atcacgagtg tcttcgtcgc gagcgcgccc
     2821 ggcggctgac cggcgcggcc cgtgcggccg acgtccacgc cttgcaacgc cggatgattg
     2881 cgaacgttct ccggtgtgtc gccgttcgga atctcccaaa gcttctcgcc cttcgtgaga
     2941 tcgaacgcgg cgatcgtgcc gtacggcggt ttcagaagtg gcaagccttg gacgctgacg
     3001 ttgcgcaaac cgccgaacgc gcccgcgtcg ggccccgtgc aaacgccgtg cggcatgacg
     3061 gagccttcgg cgtagcggca gatcgtcatg ctgttgacgc cgacgctcgc cgtcacgaag
     3121 acgatcttgt tctcggggtc gtaggagccg cccggccagt tgctgccgcc catgaccatc
     3181 agcgtgctga cgggcccgtt cggattgaac ggcacggccg gcgtgtagat gggaccgatg
     3241 cgatggttcg cgatgacgcg ctcggcctcc gcgcgcagcg ccggcgtgaa gtcgatgagg
     3301 tcgtcggcgg tgatgccttg atggccgtaa cgcagcgtgg gaatcggctg cgtcggcgaa
     3361 taccattcgc cgggcacgtt gccttgcggc acggcgactt cgggaatcgg ccaaacgggc
     3421 tcgcccgtct cgcggttcag cacgaacaag agcccctgct tcgtcgcttg cgcgagcgct
     3481 ttgaccgtcc gcccatcctt gacggcgtcg acgaggatcg gcgccgtcgg cagatcgtag
     3541 tcccacagcg gatggtgcga cgtttggaag tgccagcggc gctcgccggt ctcgagatcg
     3601 acggcgacga cgctctcgcc atagaggttc gcgccgggac gcaaaccgcc gtacatgtcg
     3661 ccgtacggcg attcgacagg cagatacgcg agcccgaggt cttcgtcggc cgcgatcgtc
     3721 gcccagacgc cggcattgcc gccgcccgtg cgggccgaat cgttgagcca cgtctcgtag
     3781 cccggctcgc ccgggcgcgg caccgtatgg aacgtccaca gcagcttgcc cgtcacgacg
     3841 tcgaacgcgc ggatgtagcc gacgacgttc ttggcctgat ccggcggcgc gagcggcgtg
     3901 tgcgcggccg gcacgatgac ggtgttgccg acgatcgtcg gcg
//
LOCUS       DATLEE010000672         3642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3506964, whole genome shotgun sequence.
ACCESSION   DATLEE010000672 DATLEE010000000
VERSION     DATLEE010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3506964"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VM692_14705"
     CDS             <1..471
                     /locus_tag="VM692_14705"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HUQ53474.1"
                     /translation="LPQGERGELRVRGPQMGAGYRNQPEETARTMRDGWVHTGDIGYV
                     DEEGFVFLVDRKKDMVIVGGYNVYPREVDEVLFKHPKIREAATVGKRDQRLGEVLVAF
                     VVLDAGATLTEEEFFEYCKTEMVKYKRPVEVHFVEALPKTGTNKINRRALRELV"
     gene            477..1577
                     /locus_tag="VM692_14710"
     CDS             477..1577
                     /locus_tag="VM692_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009803035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HUQ53475.1"
                     /translation="MALKRPTWPAGRVVTLEHTSRVLLDNPLGDPHVRMLDVWLPPQY
                     DAGKGRGLGKRFPVLFDLVGFTNSGRAHTAGRNFDENVPERAARLVHERKLGPVIIVF
                     PDCFTAFGGNQYVNSSAVGRYADYLTRELVPFVDRELRTLAAREHRGVFGKSSGGYGA
                     LLHAMKYPRYWGAAASHSGDAGFDSVYAPEWPAILDELAKQRTPRREAGPFGVRTAAG
                     GDGADDGRIARFLQHVWAKRQPNGAEIMALSGLAMAATYDPDPKAPNGFRVPFDLETG
                     ERLPQRWRRWLRHDPVELVARFARNLRTLRGLYVDCGWRDQYRLHYGNRQLSLRLTEH
                     GVPHVYEEFDGTHSGVDHRMERSLPFLFRALR"
     gene            1849..2328
                     /locus_tag="VM692_14715"
     CDS             1849..2328
                     /locus_tag="VM692_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010674199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HUQ53476.1"
                     /translation="MSTLQPDRYEDGRPMLLAGIRRQHTFAGMGKDIPRQWDDFLELG
                     TLPEQVGATAYGAICGGDSQTQTMEYMCAVEVRSFDSVPKDLGRMRVPAVRYAVFRHA
                     GNVATIQSTWQEVLSKWLPSSGLRSANTPDFEVYGERFDGATGDGGVEIWLGVEPAK"
     gene            2340..3041
                     /locus_tag="VM692_14720"
     CDS             2340..3041
                     /locus_tag="VM692_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53477.1"
                     /translation="MRSIVIVLSVIGAQAALAADDGAADIGYATVGATLEALRSDPSA
                     QFHEQRGWTVVASREGDVAVEWFFTPEGHAAHPAVVKRTATERDGIGMIDLVALCQAE
                     QSACDQLLADFRQQHEAEQAAARPELVKLDVSIALDDHERLRVERLLAEEGMAAEIRF
                     TDVLKMVIVPTLDDVGRVLLWTAVYEFDGDGYVLLGEPQLTRPGSGTATLEMTSSSGS
                     RFGFSLASLLARTVE"
     gene            3120..>3642
                     /locus_tag="VM692_14725"
     CDS             3120..>3642
                     /locus_tag="VM692_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HUQ53478.1"
                     /translation="MISAREALDRLRDGNRRFVAERQRDGRTGATRRNELTERQEPFA
                     IVLGCSDSRVPAEIVFDQGLGDLFVIRVAGNIVAPSQIGSVEFAADRFHTRLVVVLGH
                     SGCGAIQATVEQLTRPVEMQSRNLRAIVDRVRPSVEPLLATPLRDDIQALQRDAVRAN
                     VRASASHLRHGSEL"
BASE COUNT          577 a         1200 c         1262 g          603 t
ORIGIN      
        1 ttgccccagg gtgagcgcgg cgagctgcgt gtgcgcggcc cgcagatggg ggccggttac
       61 cgcaatcagc ccgaggaaac ggcgcgaacg atgcgcgacg gctgggtcca cacgggcgac
      121 atcggctacg tcgacgagga aggcttcgtg ttcctcgtcg accgcaagaa ggacatggtc
      181 atcgtcggcg gttacaacgt ttatccgcgc gaggtcgacg aggtgctgtt caagcacccg
      241 aagattcgcg aggcggctac ggtcggcaaa cgcgaccagc ggctcggaga agtgctcgtg
      301 gcgttcgtcg tgctcgatgc cggcgcgacg ctgaccgaag aagagttctt cgagtactgc
      361 aagaccgaga tggtcaagta caagcggccg gtcgaggtgc acttcgtcga ggcgctgccg
      421 aagacgggca cgaacaagat caaccgccgc gcgctgcgcg agctcgtcta gccgccatgg
      481 cgttgaagcg cccgacgtgg ccggcggggc gcgtcgtgac gctcgagcac acatcgcggg
      541 tgctgttaga caatccgctc ggcgacccgc acgtgcgcat gctcgacgtt tggctgccgc
      601 cgcagtacga cgccggcaaa ggccgcggtc tcggcaagcg ctttcccgtg ctgttcgatc
      661 tcgtgggctt cacgaacagc ggccgcgcgc acacggctgg gcgcaatttc gacgagaacg
      721 ttcccgagcg cgccgcgcgg ctcgttcacg agcgcaagct cggtcccgtc attattgtct
      781 ttccggattg cttcacggcg ttcggcggca accaatacgt gaactcgagc gccgtcgggc
      841 gctacgccga ttacctgacg cgcgagctcg tgccgttcgt cgaccgcgag ctgcgaacac
      901 tggctgcacg cgagcaccgc ggcgtattcg gcaagtcgtc gggcggctac ggtgcgctgc
      961 tgcatgcgat gaagtacccg cgctattggg gtgcggccgc ctcgcactcg ggcgatgccg
     1021 gcttcgactc tgtgtacgcg cccgagtggc ccgcgattct cgacgagctc gcgaagcagc
     1081 gcacgccgcg gcgcgaggcc gggccgttcg gcgtgcgcac tgctgccggc ggcgacggcg
     1141 cggacgacgg gcgcatcgcc cgtttcctgc agcacgtctg ggccaagcgg cagccgaacg
     1201 gcgcggagat catggcgctg tcgggtcttg cgatggccgc aacgtacgat ccggatccga
     1261 aggcgccgaa cggcttccgt gtgccgttcg atctcgagac cggggaacgc ttgccgcaac
     1321 gctggcggcg ctggctgcgg cacgatcccg tcgagctcgt cgcgcgcttt gcccgcaact
     1381 tgcgcacgtt gcgcggcctc tacgtcgatt gcggctggcg cgaccagtac cggctgcact
     1441 acggcaaccg tcagctgtcg ttgcggttga cggagcacgg cgtgccgcac gtttacgagg
     1501 agttcgacgg cacacactcg ggcgtagacc accgcatgga gcgcagcttg ccgtttctct
     1561 tccgcgcatt gcgctgagca cgagggcacc gacgacgctg ttgcaagtca tcggcgatgt
     1621 ggccttggcc gccgccgtgg gccgtcccac cttcgcgtaa ccgaacccgc gagttcgcgc
     1681 gccgcacgcg ttcggccgac gtttgcagcg ctgtgcctcg gcggcggctg gtggcttgga
     1741 tcgctcgcgc gtaatgtccg cctactagtc gcgtcggctc gcgtcgtgca tcgatcgtgc
     1801 aacgatcgcg gatagcgata ggtgcacgag cgttctatcc ttggagtcat gagcacgctg
     1861 caacccgatc gctacgagga cggccggccg atgctgctcg ccggtattcg ccgccagcac
     1921 acgttcgcgg gcatgggcaa ggacattccg cgccaatggg acgatttcct cgagctcggc
     1981 acgctgccgg agcaggtggg cgcgacggcg tacggcgcga tctgcggcgg cgattcgcag
     2041 acgcaaacga tggaatacat gtgtgccgtc gaggtccgta gcttcgactc ggtgccgaag
     2101 gatctcggtc gcatgcgcgt gcccgcggtg cgctatgccg tgttccgcca cgcaggcaac
     2161 gtcgcgacga ttcaaagcac gtggcaagag gtcttgtcga agtggctgcc gagctctgga
     2221 ctgcgttcgg cgaacacgcc ggacttcgag gtttacggcg aacgcttcga cggtgcgacg
     2281 ggcgacggcg gcgtcgagat ttggcttggt gttgaacccg cgaaatgagg aagcagcgca
     2341 tgcgcagcat cgtgatcgtg ttgagcgtca tcggcgcgca ggcggcactc gcagccgacg
     2401 acggcgccgc agatatcggc tacgcgaccg tcggcgcgac gctcgaggcg ctgcgcagcg
     2461 atccgagcgc gcagttccac gagcaacgcg gttggaccgt cgtcgcgagc cgcgaaggcg
     2521 acgttgccgt cgagtggttc ttcacgcccg agggccatgc ggcgcacccg gccgtcgtca
     2581 agcgcacggc cacggagcgc gacggtatcg gcatgatcga cctcgtagcg ctgtgtcagg
     2641 ccgagcaaag cgcctgcgat cagctgctgg ccgattttcg ccagcagcac gaggccgagc
     2701 aggccgccgc acggcccgag ctcgtcaagc tcgacgtcag catcgcgctc gacgatcacg
     2761 agcgcttacg cgtcgagcgc ctgctggccg aagagggaat ggccgcggag attcgcttca
     2821 cggacgtctt gaagatggtc atcgtgccga cgctcgacga cgtcggccgc gtgctgttgt
     2881 ggacggcggt ctacgaattc gacggcgacg gctacgtgct gctcggcgag ccgcagttga
     2941 cgcgccccgg cagcggcacg gccacgctcg agatgacctc gtcgtcgggt agccgcttcg
     3001 gcttctcgct ggcttcgctg ctcgcgcgaa ccgtcgagta ggctccgccg cgctcgattt
     3061 cgcggcaagc aatgctaaag tgcgcgccca gcgcaacgtg tggcgctcgg gaggtggcga
     3121 tgatttcagc gcgtgaggcg ctcgaccggc tgcgcgacgg caatcgacgc ttcgtggccg
     3181 aacgccagcg cgacggacgc acgggcgcga cgcggcgcaa cgagctcacg gagcggcaag
     3241 agccgttcgc catcgtgctc ggctgctccg actcgcgagt gcccgccgaa atcgttttcg
     3301 accaaggcct cggcgacctg ttcgtcatcc gagtcgccgg caatatcgtc gcgccgtcgc
     3361 aaatcggcag cgtcgagttc gcggccgatc gcttccatac gcggctcgtc gtcgtgctcg
     3421 gccactcggg ctgcggcgcg atccaggcga ccgtcgagca gctaacccgc cccgtcgaga
     3481 tgcagtcgcg caacttgcgc gcgatcgtcg atcgcgtgcg gccgtccgtc gagccgctgc
     3541 tcgcaacacc gctgcgcgac gacatccagg cgctgcagcg cgacgccgtg cgcgccaacg
     3601 tgcgcgcgtc cgcaagccat ctgcgtcacg gctccgagct gc
//
LOCUS       DATLEE010000673         2527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10946044, whole genome shotgun sequence.
ACCESSION   DATLEE010000673 DATLEE010000000
VERSION     DATLEE010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10946044"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VM692_14730"
     CDS             <1..642
                     /locus_tag="VM692_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HUQ53479.1"
                     /translation="LAPQGAAWDAALKRWRALPTDAGAKFDKEVHIDVAKLRPMVTFG
                     TNPGMVIPIDEAVPHDDDPAFRKALAYMQIEAGKPLLGKPVDVVFIGSCTNGRLSDIR
                     QAASILRGRRVAERVRLLVVPGSHDVKRQAEAEGLDEVVRAAGGEWREPGCSMCIGMN
                     GDTVPSGKLSVSTSNRNFEGRQGSGARTVLASPLTAAAAAIKGAIADPRELVS"
     gene            644..1216
                     /gene="leuD"
                     /locus_tag="VM692_14735"
     CDS             644..1216
                     /gene="leuD"
                     /locus_tag="VM692_14735"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HUQ53480.1"
                     /translation="MQPIKEIRSKTVVLPNDDIDTDQIIPARFLTTTTKEGLGKNLFN
                     DWRYTKDGRENSEFALNKPENRGAAVLVGGNNFGCGSSREHAPWALLDFGIRAVVSVG
                     IADIFRSNSLKNGLIPIIVDKDSHRWLLEHPGADVHIDLKSATLAMNGKTVSFPIDRF
                     SRYCLMNGVDQLGFLLEQDAAISRYERAHA"
     gene            1213..2286
                     /gene="leuB"
                     /locus_tag="VM692_14740"
     CDS             1213..2286
                     /gene="leuB"
                     /locus_tag="VM692_14740"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HUQ53481.1"
                     /translation="MSKHIVVLAGDGVGPEVTAEAVRVLEQVSTTHKLGLEFENALIG
                     GAAYDAAGSPLPEKTVAACKASTGILLGAVGGPKWADIPVQHRPEQGLLAIRKALGLF
                     ANLRPVATMPELAAASPLKPEKLKNVDILVIRELTGGIYFGKRSRSADAAFDTCEYSA
                     AEIERVCRVAGELATKRRRKVTSVDKANVLDTSRLWRDVATRVFRSEFPELAVEHLLV
                     DAAAMHLLSRPADFDVMVTENMFGDILTDEASMLAGSMGLLPSASLGAGSGPGLYEPI
                     HGSAPDIAGQGVANPCGTILSAALLLRHSCGAEGAAQAIEAAVAGAIRAGARTRDIAA
                     PGARDWLGTTAFTDAVLKQLKAH"
     gene            complement(2342..>2527)
                     /locus_tag="VM692_14745"
     CDS             complement(2342..>2527)
                     /locus_tag="VM692_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53482.1"
                     /translation="RVPFFYYLLHIPAAHLAACIVSLVREGRVDPWLFGNHPMMSGPA
                     PEGYMWGLPLLYLKITR"
BASE COUNT          451 a          861 c          830 g          385 t
ORIGIN      
        1 ctcgcgccgc agggcgcagc gtgggatgcg gccctgaagc gttggcgcgc gttgccgacg
       61 gatgccggcg cgaagttcga caaagaagta cacatcgacg tggccaagct gcggccgatg
      121 gtcacgttcg gcacgaatcc cggcatggtc attccgatcg acgaggccgt gccgcacgac
      181 gacgatccgg cgtttcgcaa agccctcgcc tacatgcaga tcgaggccgg caagccgctg
      241 ctcggcaaac ccgtcgacgt cgtcttcatc ggctcgtgca cgaacggccg cttgagcgac
      301 attcggcagg ccgcgagcat cctgcgcggc cggcgcgtcg ccgagcgcgt ccgactgctc
      361 gtcgtgccgg gctcgcatga cgtcaagcgg caggccgagg ccgaaggcct cgacgaggtc
      421 gtgcgcgccg caggcggcga gtggcgcgag cccggctgct cgatgtgcat cggcatgaac
      481 ggcgacacgg tgccgagcgg caagctgtcc gtcagcacga gcaaccgcaa cttcgaaggc
      541 cggcaaggca gcggcgcacg caccgtgctc gcgagcccgc tgacggcggc agctgccgca
      601 atcaaaggcg cgatcgcgga tccgcgcgag ctcgtgagct gacatgcaac cgatcaaaga
      661 gattcgctcg aagaccgtcg tcttgccgaa cgacgacatc gacaccgatc agatcattcc
      721 ggcgcgcttc ctgacgacca cgacgaagga agggctcggc aagaacctgt tcaacgactg
      781 gcgttacacg aaggacggcc gcgagaactc agagttcgcg ctgaacaagc ccgagaaccg
      841 cggtgcggcg gtgctcgtcg gcggcaacaa tttcggctgc ggctcgtcgc gcgagcacgc
      901 accgtgggcc ttgctcgact tcggcattcg cgccgtcgtc agcgtaggaa tagccgacat
      961 cttccgcagt aactcgctga agaacgggct gatccccatc atcgtcgaca aggacagcca
     1021 ccgctggctg ctcgagcatc ccggtgccga cgtgcatatc gatttgaagt ccgcgacgct
     1081 cgcgatgaac ggcaagaccg tctcgttccc gatcgaccgc ttctcgcgct actgcctcat
     1141 gaacggcgtc gaccagctcg gtttcctgct cgagcaggac gcggcaatca gccggtacga
     1201 gcgagcacac gcatgagcaa gcacatcgtc gtattggccg gcgacggcgt cggccccgag
     1261 gtcacggccg aggccgtgcg cgtgctcgag caagtctcga cgacgcacaa gctcgggctc
     1321 gagttcgaaa atgcattgat cggcggcgcg gcttatgacg ccgcaggctc gccgctgccc
     1381 gaaaagaccg tcgcggcctg caaagcctcg acgggcattc tgctcggcgc cgtcggcggc
     1441 ccgaagtggg ccgacatccc cgtgcagcat cgccccgagc aaggcttgct cgcgatccgc
     1501 aaggcgctcg gcctgttcgc gaatttgcgg cccgttgcca cgatgccgga gctcgcggcc
     1561 gcttccccgc tgaagcccga gaagctgaaa aacgtcgaca tcctcgtgat ccgcgagctg
     1621 acgggcggca tctatttcgg caagcggtcg cgctcggccg acgccgcgtt cgatacctgc
     1681 gagtactcgg ccgccgagat cgagcgcgtc tgccgcgtcg cgggcgagct tgcaacgaag
     1741 cgccgccgca aggtcacgtc ggtcgacaaa gccaacgtgc tcgacacgtc gcggttgtgg
     1801 cgtgacgtcg cgacgcgcgt gttccgcagc gagtttcccg agctcgccgt cgagcacttg
     1861 ctcgtcgatg cggccgccat gcacctgttg agccggcccg ccgacttcga cgtcatggtc
     1921 accgagaaca tgttcggcga catcttgacc gacgaagcgt cgatgctcgc cggctcgatg
     1981 gggcttctgc cctcggcttc gctcggcgcc ggcagcggcc ccgggcttta tgagccgatc
     2041 cacggctcgg cgcccgacat cgccgggcaa ggcgtcgcaa acccgtgcgg cacgatcctg
     2101 agcgccgcgc tgctgctacg ccatagctgc ggcgccgagg gcgcggccca agccatcgag
     2161 gcagccgtag ccggcgccat tcgtgcgggc gcacgcacgc gcgacatcgc ggccccgggc
     2221 gcgcgcgatt ggctcggcac gacggctttc accgacgccg tcctgaagca actcaaggct
     2281 cattgacctc gagcttgacc ggccccgacg cgctgagcta atgtagtaac gcgctattac
     2341 attagcgcgt tatcttgaga tacagcagcg gcagccccca catgtaccct tcaggcgccg
     2401 ggccgctcat catcgggtgg ttgccgaaca gccacggatc cacgcgcccc tcgcgcacga
     2461 gcgacacgat gcacgcggcg aggtgggcgg cggggatgtg gagcaggtaa tagaagaagg
     2521 gcacccg
//
LOCUS       DATLEE010000674         4670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8714142, whole genome shotgun sequence.
ACCESSION   DATLEE010000674 DATLEE010000000
VERSION     DATLEE010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8714142"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VM692_14750"
     CDS             complement(<1..448)
                     /locus_tag="VM692_14750"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HUQ53483.1"
                     /translation="MPKKGVSPGDRLKLDNPAHILGILLKALQHTLRQRFDEALRKQG
                     VELSFAQFAALLNLHSDPGVTGAALARRAMVSAQTMNSALRALERDGYIERRPHPLSR
                     RADSWSLTGRGLDELQRARQVGSAIFARMLKDFDATEVAAFEDYLRR"
     gene            521..1672
                     /locus_tag="VM692_14755"
     CDS             521..1672
                     /locus_tag="VM692_14755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUQ53484.1"
                     /translation="MSLHASRPLVVPEGGHWGRRAAASFILIGALLSVSACGRQENAA
                     ADARGAAAREAVMTVTATPLERVDVTRTISMNGSVYAWQDVIISSEVGGYRVAEVLVD
                     VGARVRKGQRLVSLSTALLEAEVATKQAALKQREAELMNTESALERGRSLGERNLLSK
                     ADLDRLTSEQLASQSRLESARADLETSQLRLKFTDVVAPDDGIITSRTVTVGQIAQAG
                     AEMLRLLRNGRIEWRGQAPETRLNDLQPGQGVSVMTADGTPFHGKIRVVSPTISANDR
                     TALVYVDLPADERLRPGMFARGEIAIGTSPAAMVPLESVVSSDGYSYVFVLSEDNTVA
                     RRRIETGAIHDGSIEVVSGVDPGERIVNKGAGFLKDGDLVSVSTAVSGS"
     gene            1669..>4670
                     /locus_tag="VM692_14760"
     CDS             1669..>4670
                     /locus_tag="VM692_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUQ53485.1"
                     /translation="MNFVTWSIRNPVPVIVLFIALTVAGLISFPKLGVLDRPDIEFPA
                     VIVTVTYPGVAPTQMESEVTRKVEDAVATIAGIEQMTSTVDEGASTTSIEFRFGTDLS
                     AALDDVRDAMTRIRSDLPADANEPIVSRVTTAGGAIVTWSVASDNMTATELSWFVDLT
                     ITRELSAVPGIGRVSRVGGVAREVRVDLDPDRMAALGATASDVSRQLRRIQAEYPGGE
                     GRLGGLEQTVRTTGIITSVYDLRALPISLPDGRSVRLETIAEVRDQAGEQRSAALLDG
                     EPVIGFQIVRSWGASALDVALDARAVVERLQQEYPQVKFAEASSQVAYIQESFNASME
                     MLVEGAILAILVVWLFLRDWRATAVSAIALPLSIIPTFWAVWALGYTLNILTLLALSL
                     VVGILVDDAIVEVENIVRHLRMGKKPKEAAMDAAIEIGLAVVATTLTICAVFIPVAFM
                     SGIAGEFFGPFSFTVVVAVLCSLLVARTLTPMMAAYFLKPHDRPEKPARIMTWYLERV
                     RWCLAHRWMTLGVATLAIGSMLALAPFLSTAFSPAGSNGFTTLSVELAPGASLADTLA
                     AAETARRRLEGLPETRSVYTTIGAASGGNSPTGGGSAGNVRRGSLVIQLDNPDGTRGA
                     QQIYERKATELLRDIPGARFQFGSGGGGSRLQVTLAGDEANRLNLAAANVEREIRAMP
                     GLGMITSSAALQKPEIVIRPSPERAAELGVTTETLALVTRIATSGDVDISLAKFNLDN
                     RQIPIRVRLNDASRTDLERLQLLSVPGRNGPVPLMNIADVSLGAGPAQITRIDRSRNI
                     TLNVDLNEQALGDTLETIESLPSVTNLPDGVRLVPTGDARWIREIFGEFALAMAIGVL
                     SIYAVLVMLFHKFIQPVTILAALPPSAGGAVLALFAFDYDLAINSLIGLLMLMGIVSK
                     NSILIVEYAIMAQRDQGLSRFESLIDSCSKRARPIVMTTIAMIAGMTPIAMGWAGDPS
                     FRVPMGIAVIGGLIVSTIMSLFIVPAVFT"
BASE COUNT          735 a         1569 c         1557 g          809 t
ORIGIN      
        1 agcgccgcag gtagtcctcg aacgccgcga cctcggtcgc atcgaaatcc ttcaacatgc
       61 gcgcaaaaat cgccgagccg acttgccgcg cgcgctggag ctcgtcgagc ccacggcccg
      121 tcaacgacca gctgtcggcg cggcggctca gcggatgtgg acggcgctcg atgtagccgt
      181 cgcgctcgag ggcgcgcagc gccgagttca tggtttgagc cgataccatc gcgcgacgcg
      241 ccagtgcggc gcccgtgacg ccagggtcgc tgtgcagatt caagagggcg gcgaactgcg
      301 caaacgacag ctcaacccct tgtttccgca gagcctcgtc gaaccgctgg cgaagcgtgt
      361 gctgcaacgc cttcagcaag atccccagga tgtgtgcggg gttatcgagc ttcagccggt
      421 cgccggggga tacacctttc ttgggcatcg atgcagttgc cacgtatcag atagctgata
      481 tcatatcagc aagctgatac gtatcaagga cgacttccac atgtccctcc atgcatcgcg
      541 ccccttggta gtccccgaag gggggcactg gggccgtcgg gccgctgcgt cgtttatttt
      601 gatcggtgct ttgctctccg tgtcggcctg cggccggcaa gagaacgccg ccgccgatgc
      661 gcgtggagcg gccgcacgcg aggccgtgat gacggtcacg gccacgccgc tcgagcgcgt
      721 cgacgtcacg cgcacgatct cgatgaacgg ctccgtctat gcatggcaag acgtcatcat
      781 ttcctcggaa gtcggcggct atcgcgtcgc cgaggtgctc gtcgacgtgg gcgcgcgcgt
      841 gcgcaaaggt cagcgcctcg tctcgttgtc gacggccttg ctcgaggccg aggtcgcgac
      901 taagcaagcg gcgttgaagc agcgcgaggc cgagctcatg aacacggagt cggcgctcga
      961 gcgcggccgg tcgctcggcg agcgaaactt attgtcgaaa gccgacctcg accgcctgac
     1021 gtcggagcag ctcgcgtcgc aatcgcggct cgaatccgcg cgcgccgacc tcgagacgtc
     1081 gcagctgcgg ctcaaattca cggacgtcgt ggcgcccgac gacggcatca tcacgtcgcg
     1141 cacggtcacg gtcggtcaga tcgcgcaggc cggcgccgag atgctgcgcc tcttgcgcaa
     1201 cggccgcatc gagtggcgcg gccaggcgcc cgagacgcgc ctcaacgatt tgcagcccgg
     1261 ccaaggcgtc agcgtcatga cggccgacgg cacgccgttc cacggcaaga ttcgcgtcgt
     1321 ctcgccgacg atctcggcga acgaccgcac ggcgctcgtc tacgtcgatc tgcccgccga
     1381 cgagcgcttg cggcccggta tgttcgcgcg cggcgagatt gcgatcggca cgagcccggc
     1441 cgcgatggtg ccgctcgaga gcgtcgtgag ctcggacggc tacagctacg tgttcgtgct
     1501 cagcgaggac aacacggttg cgcggcggcg aatcgagacc ggcgccatcc atgacggctc
     1561 gatcgaggtc gtcagcggcg tcgaccccgg cgagcgcatc gtgaataaag gcgccggctt
     1621 cctgaaggac ggcgacctcg tcagcgtcag cacggcggtg agcggctcat gaacttcgtc
     1681 acctggtcga tccgcaaccc cgttcccgtc atcgttctgt tcattgcgct gacggtcgcg
     1741 gggctcatca gctttccgaa gctcggcgtg ctcgaccggc ccgacatcga gttccctgcc
     1801 gtcatcgtca cggtcacgta tcccggcgtt gcgccgacgc agatggaatc ggaggtgact
     1861 cgcaaggtcg aggacgccgt cgcgacgatc gccggcatcg agcaaatgac gtcgacggtc
     1921 gatgagggcg catcgacgac gagcatcgaa ttccgcttcg gcacggatct gtcggcggcc
     1981 ctggacgacg tgcgcgacgc gatgacgcgc attcgctccg atctgccggc cgacgccaac
     2041 gagcccatcg tctcgcgcgt cacgacggcc ggcggcgcga tcgtcacgtg gagcgtcgcc
     2101 tcagacaaca tgacggccac ggagctttcg tggttcgtcg atctcacgat cacgcgcgag
     2161 ctgtcggccg tgcccggcat cggccgcgtg tcgcgcgtcg gcggcgtcgc gcgcgaggtc
     2221 cgcgtcgacc tcgatcccga ccgcatggcg gcgctcggcg cgacggccag cgacgtctcg
     2281 cgccagctgc gacgcatcca agccgaatac cccggcggcg aaggccgcct cggcggtctc
     2341 gagcagaccg tgcgcacgac gggcatcatc acctccgtct acgacttgcg cgcgctgccg
     2401 atctcgttgc cggacggccg cagcgtccgg ctcgagacga tcgccgaggt ccgcgaccaa
     2461 gccggcgagc agcgctcggc ggccttgctc gacggcgagc ccgtcatcgg cttccagatc
     2521 gtacggtcgt ggggcgcgag cgcgctcgac gtcgctttgg atgcgagggc cgtggtcgag
     2581 cgcctgcagc aggaatatcc gcaggtaaag ttcgccgagg cgagctcgca ggtcgcctac
     2641 atccaggaat cgttcaacgc gtcgatggag atgctcgtcg agggcgcgat cctcgcgatc
     2701 ctcgtcgtct ggctattcct gcgcgattgg cgcgcgacgg ccgtgtccgc catcgccttg
     2761 ccgttgtcga tcatcccgac gttttgggcc gtgtgggcgc tcggctacac gctgaatatc
     2821 ttgacgttgc tcgcgctgtc gctcgtggtc ggcatcttgg tcgacgacgc aatcgtcgag
     2881 gtcgagaaca tcgtgcggca cttgcgcatg ggcaagaagc cgaaagaagc ggcgatggac
     2941 gccgcgatcg agatcggcct cgcggtcgtc gcgacgacgc tgacgatctg cgccgtgttc
     3001 atccccgtcg cgttcatgag cggcatcgcg ggcgagttct tcggcccttt cagcttcacg
     3061 gtcgtcgtgg ccgtgctgtg ctcgctgctc gtcgcgcgga cgctgacgcc gatgatggcg
     3121 gcatatttcc tgaagccgca tgaccggccg gaaaagccgg cacgcatcat gacgtggtat
     3181 ctcgagcgcg tccgctggtg cctcgcgcat cgttggatga cgctcggcgt cgcgaccctc
     3241 gcgatcggct cgatgctcgc cctcgctccg ttcctgtcga cggccttctc gccggccggc
     3301 agcaacggct tcacgacgtt gagcgtcgag ctcgcgccgg gcgcgtcgct agccgacacg
     3361 ctcgcggccg ccgaaaccgc gcgccggcgt ttggaaggcc tgcccgagac gcgcagcgtc
     3421 tacacgacga tcggcgctgc cagcggcggc aacagtccga cgggcggcgg ctcggccggc
     3481 aatgtccgcc gcgggtcgct cgtgattcag ctcgacaacc ccgacggcac gcgcggcgcg
     3541 cagcagatct acgagcgcaa ggccacggag ctcttgcgcg acattccggg ggcgcgcttt
     3601 cagttcggca gcggcggcgg cggcagccgg ctgcaagtca cgcttgccgg cgacgaggcc
     3661 aatcggctga atctcgcggc cgcgaacgtc gagcgcgaaa ttcgtgcgat gccggggctc
     3721 ggcatgatca cgagcagcgc cgcgctgcaa aagcccgaga tcgtgattcg gccgtcgccg
     3781 gagcgcgcgg ccgagctcgg tgtcacgacg gagacattgg ccctcgtcac gcgcatcgcg
     3841 acgagcggcg acgtcgacat cagcctcgcg aagttcaacc tcgacaatcg ccagattccg
     3901 atccgcgtgc gtttgaacga cgcttcgcgc accgatctcg agcggctgca gctgttgtcg
     3961 gtgccgggcc gcaacggccc cgtgccgctc atgaacatcg ccgatgtctc gctgggcgcg
     4021 ggcccggcgc agatcacgcg catcgaccgc agccgcaaca tcactttgaa cgtcgacttg
     4081 aacgagcagg cgctcggcga cacgctcgag acgatcgagt cgctgccttc cgtgacgaac
     4141 ttgcccgacg gcgtgcgctt ggttccgacc ggcgacgcgc gctggatccg cgagatcttc
     4201 ggcgagtttg cgctcgcgat ggcgatcggc gtgctgtcga tctacgccgt gctcgtcatg
     4261 ctgttccaca agttcataca gcccgtcacg atcttggcgg cgctgccgcc gtcggccggc
     4321 ggcgccgtcc tcgcgctgtt cgcgttcgac tacgacctcg cgatcaactc gctgatcggc
     4381 ttgctgatgc tgatgggcat cgtgtcgaag aactccatct tgatcgtcga gtacgcgatc
     4441 atggcgcagc gtgatcaagg cttgtcgcgg ttcgagtcct tgatcgattc gtgctcgaag
     4501 cgcgcacggc cgatcgtcat gacgacgatc gcgatgatcg cgggcatgac gccgatcgcg
     4561 atgggttggg ccggcgaccc gagcttccgt gtgccgatgg gtatcgccgt catcggcggc
     4621 ttgatcgtct cgacgatcat gagcttgttc atcgtgcccg ccgtgttcac
//
LOCUS       DATLEE010000675         4999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10948122, whole genome shotgun sequence.
ACCESSION   DATLEE010000675 DATLEE010000000
VERSION     DATLEE010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10948122"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..631)
                     /locus_tag="VM692_14765"
     CDS             complement(11..631)
                     /locus_tag="VM692_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HUQ53486.1"
                     /translation="MQITDMLAQMGGLRSIASELGVSETQVATGATALAPAILGGFKN
                     QARSQPSGLEGLGGLLGKLGGGALLDDVLKPQRTDVSRGNDVLGHIFGSKEVSRTVAQ
                     DAASKTGLSPDLLKKMLPMMAMLVAGYMAKQRNANAGAAAQDGPASAPAAPTGGGLGN
                     VLGGLRGGGATGAHRTGLGGIGGMLDMDGDGNALDDILRMAGKKLR"
     gene            complement(652..1044)
                     /locus_tag="VM692_14770"
     CDS             complement(652..1044)
                     /locus_tag="VM692_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HUQ53487.1"
                     /translation="MSVFGTILAKLGVGKGTGAPNPPPQGMPPTSSSPTPTASSSPAA
                     TVDVAAQLEQRAATNPQKLNWRTSIVDLLKLLDMDSSVSARKTLAKELGCPAELMGDS
                     AKMNKWLHKTVLAQVAANGGKVPQDLIH"
     gene            complement(1193..3250)
                     /locus_tag="VM692_14775"
     CDS             complement(1193..3250)
                     /locus_tag="VM692_14775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HUQ53488.1"
                     /translation="MKYLPLAAACTVLGLAACSDDGAVAPAATPAVATAKPAFGTFGI
                     DTTAMDTSVKPGDDFYRYVNGKWVATFQMPPDKARYGAFDALRDKSETDVRTLLDELA
                     AAPRPAGSVQQKVADLYGSWMDEAAIEARGIEPLKADLETIAAARTKRDIVALMGKTD
                     YASPVALYISPDPADTTRYVVNVTQAGLGMPVRDYYLNEGAKFDAYRSQYRVYVTKIF
                     ELIGDGNPAASAEAVVALETELAKVHWSPERRRDVQATNNPVDRAGLLAMIPAVDWDA
                     LLPVAGLGAVQHFVVNETTALHDGAALLDTQPVETWKKYLAFHLADTYASDLPKAFDD
                     ASFEFYRRTLSGVEVQRDRWKRGVQLLDGLIGEGVGELYVAKYFPPDYKAKMDELVAN
                     LRLAMGQRLSTLSWMDEATRAEAQKKLGTFDPRVGYPVKWRDYSAYTVERGKLFENVR
                     NGREFEWKRQVERLDEPVDRDEWGMNPQTVNAYYNPLVNQITFPAAILQPPFFDPNAD
                     LAVNYGAIGAVIGHEMGHGYDDQGREYDESGKIRNWWTPETNDKFMAAISMFAAQYNA
                     FCPLDGACVNGNFTMGENIGDLGGLEMAYTAYKIALKGAEAPVIDGFTGDQRFFMAHA
                     QVWRGIQREDALRSQMLTDPHSPAPARGSIPERNMDAWYAAFNVKEGDAQYLAPENRV
                     RIW"
     gene            complement(3344..3865)
                     /locus_tag="VM692_14780"
     CDS             complement(3344..3865)
                     /locus_tag="VM692_14780"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HUQ53489.1"
                     /translation="MSGTQQKATQLYDLIDGIEIAMFTTRRPDGRLVSRPMATQARAA
                     GADLWFVTDASAYKLDELEQEPHVNLAYYNQKSREYVSVSGTGRISNDRAKIAELYRP
                     DWKAWFDGDSKEAGTPDDPRIVLIAVDVESAVYLKVDKPRPVVLYEVVKGMLTGSTPD
                     LGETQRVSGRELH"
     gene            complement(3980..>4999)
                     /locus_tag="VM692_14785"
     CDS             complement(3980..>4999)
                     /locus_tag="VM692_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUQ53490.1"
                     /translation="TGWVYVLDRTNGTPLVGVDERAVPQEPRQGTAATQPYPRGDAFV
                     PQQIDIAPEGTVLVNEGKIFTPFWTTPVLAKPGPPGGVNWPPSSYDATSGLLFVCAGD
                     RIWSFLSQEVGPERPAEGAGFIAGGIGGFHMTSLGVFAAVDVRTNKLVWQQHWPSPCY
                     SGSLATAGGLVFVGRNDGRLTALNSSTGMKLWEFQTGAGMNSPATSFEHDGKQYVVAY
                     SAGNQFAGSARGDSLWLFGLDGALEPVPPAGSLMTFAPGGAGPGNADAGATVYTTACL
                     ACHGEQGEGGHGGGPSLQALKNRAVVLQVVSEGRNAMPAFGGGTLTAEQIRDVATYVS
                     ERLAK"
BASE COUNT          759 a         1650 c         1656 g          934 t
ORIGIN      
        1 tcagcgcagg ctaacggagt ttcttgccgg ccatgcgcag gatgtcatcc agcgcatttc
       61 cgtcgccgtc catgtcgagc atccctccga taccgcccaa gcctgttcgg tgtgcgcccg
      121 tcgcgccgcc tccgcgtaat cctccgagaa cgttgccgag tccgccgcca gtgggtgcgg
      181 caggcgccga agcgggtccg tcctgcgcgg cggcgccggc gttcgcgttc cgttgcttcg
      241 ccatgtagcc ggcgaccagc atcgccatca tcggcaacat ctttttgagc aggtccgggc
      301 tcaagcctgt cttggaggcc gcatcctgcg cgaccgtgcg actgacctcc ttcgagccga
      361 agatgtgtcc gagcacgtcg ttgccacggc tgacgtcggt ccgctgcggt tttagcacgt
      421 cgtcgagcaa tgcgccgccg ccgagcttgc cgagcaggcc gccgaggcct tcgagccccg
      481 acggttgcga ccgcgcttgg ttcttgaagc cgcccaagat tgccggtgca agtgctgttg
      541 cgccggtcgc gacttgcgtc tcgctgacac cgagctcgct ggcgatcgat cgaaggccgc
      601 ccatctgggc aagcatgtcg gtaatttgca tgtcgatgct ccctcgtgag atcagtggat
      661 gaggtcctgc ggcaccttgc cgccattcgc ggcgacctgg gccagcaccg tcttgtgcag
      721 ccacttgttc atcttcgccg agtcgcccat gagctccgcc gggcagccaa gctccttggc
      781 gagtgtcttg cgggcggaga cgctactgtc catgtcgagc aacttcagca gatcgacgat
      841 cgacgtccgc cagttgagct tctgtgggtt cgtggccgcg cgctgctcga gctgagcggc
      901 gacatcgacg gtcgctgccg gtgaactcga tgctgtcggc gtcgggctcg aggaagtcgg
      961 cggcatgcct tgcggcggtg gattcggcgc gccagttcct ttgccgacgc cgagcttcgc
     1021 gaggatcgtt ccgaatactg acatgtgcgc tctccttgcg tggtggagcg gcgcagagcg
     1081 ccgctaatct tcctttgccc agagggcaaa tcgatgggcg gagacccgcc tcgataagag
     1141 gatacgccga tcgccgccta cgatgatgcg gaactttttt tctagcgtgg ggtcaccaaa
     1201 ttcgcacgcg gttctcgggc gcgaggtact gcgcgtctcc ttctttgacg ttgaacgcgg
     1261 cataccacgc gtccatgttg cgctcgggga tcgagccgcg cgccggggcc ggtgaatgcg
     1321 gatcggtcag catctggctg cgtagcgcgt cctcgcgctg gatcccgcgc caaacctgcg
     1381 cgtgtgccat gaagaagcgt tgatcgcccg tgaagccgtc tatgacaggt gcttcggcgc
     1441 ctttcaaggc gatcttgtag gccgtgtacg ccatctcgag gccgcccaag tcgccgatgt
     1501 tctcacccat cgtgaagttg ccgttcacac aggcgccgtc gagcgggcag aacgcgttgt
     1561 actgcgccgc gaacatcgag atggccgcca tgaacttgtc gttcgtctcg ggcgtccacc
     1621 agttgcggat cttgccgctc tcgtcgtact cgcggccttg atcgtcgtag ccgtggccca
     1681 tctcgtggcc gatcacggcg ccgatggcgc catagttgac ggctagatcg gcgttgggat
     1741 cgaagaacgg cggttgcaga atcgcggccg gaaacgtgat ctgattgacg agcgggttgt
     1801 aatacgcgtt gacggtctgc gggttcatgc cccactcgtc gcggtcgacg ggctcgtcga
     1861 gccgctcgac ttggcgcttc cattcgaact cgcggccgtt gcgcacgttc tcgaacagct
     1921 tgccgcgctc cacggtgtac gcggagtagt cgcgccattt cacggggtag ccgacgcgcg
     1981 ggtcgaacgt gccgagcttc ttttgcgcct cggctcgagt cgcctcgtcc atccaggaga
     2041 gcgtcgacag gcgctggccc atcgcgagac gcaggttcgc aaccagctcg tccattttcg
     2101 ctttgtagtc cggcgggaaa tacttcgcga cgtagagctc gccgacgcct tcgccgatga
     2161 gcccgtcgag cagctgcacg ccgcgcttcc agcggtcgcg ctgcacctcc acgccggaaa
     2221 gcgtgcgccg atagaactcg aagctcgcgt cgtcgaacgc cttcggcaga tcgctggcgt
     2281 acgtgtccgc gagatggaac gcgaggtact tcttccaggt ctcgacgggt tgcgtatcga
     2341 gcagcgcggc gccgtcgtgc agcgccgtgg tctcgttgac gacgaagtgc tgcacggcgc
     2401 ccaagccggc aacgggcagc agcgcatccc aatcgacggc cgggatcatc gcgaggagac
     2461 cggcgcggtc gacgggattg ttcgtcgcct gcacgtcgcg gcgccgctcg ggcgaccagt
     2521 gcaccttcgc gagctcggtc tcgagcgcga cgaccgcctc ggcgctcgcc gccggattgc
     2581 cgtcgccgat cagctcgaag atcttcgtga cgtagactcg gtactgcgag cgataagcgt
     2641 cgaacttcgc gccttcattc aaatagtagt cgcgcacggg catgccgagg cccgcctgcg
     2701 tgacgttgac gacgtagcgc gtcgtgtcgg ccgggtccgg cgagatgtag agcgcgacgg
     2761 ggctcgcata gtccgttttg cccatgagcg cgacgatgtc gcgtttggtc cgcgccgcgg
     2821 cgatggtctc gaggtcggct ttgagcggct cgatgccgcg cgcttcgatt gcggcttcgt
     2881 ccatccatga gccataaagg tcggcgacct tttgctgcac ggagcccgct gggcgcggag
     2941 ccgcagccag ctcgtcgagc aacgtgcgga cgtcggtttc ggacttgtcg cgcaacgcat
     3001 cgaacgcccc gtaccgcgcc ttgtcgggcg gcatttgaaa cgtagcgacc catttgccgt
     3061 tgacgtagcg atagaagtcg tcgccgggct tgaccgacgt gtccatcgcc gtcgtgtcga
     3121 tgccgaacgt gccgaacgcg ggtttcgccg tagcgaccgc gggcgtagcg gcaggcgcga
     3181 cggcgccatc gtcgctgcag gcggctaaac ccaagaccgt gcaagcggcg gccaacggca
     3241 aatacttcat acgggcagct ccgtccgaaa cgggggctcc gagtatagcc ttgccgcagc
     3301 agggacagcg cgcggcgggc cgcggccgcc taggcgccgg ggatcagtgc aattcgcggc
     3361 cgctgacgcg ttgcgtttcg ccgagatcgg gcgtgctgcc ggtcagcatc cctttgacga
     3421 cctcatagag gacgacgggc cgcggcttgt cgaccttcaa gtacaccgcc gattcgacgt
     3481 cgaccgcgat cagcacgatg cgcggatcgt cgggcgtgcc ggcttccttc gaatcgccgt
     3541 cgaaccatgc tttccaatcc ggccgataga gctcggcaat cttcgcgcgg tcgttcgaaa
     3601 tgcgccccgt gccgctcacg gacacgtact cacgcgactt ctggttgtag taggcgagat
     3661 tgacgtgcgg ctcttgctcg agctcgtcga gcttgtaggc agacgcatcg gtcacgaacc
     3721 acaggtccgc gcccgccgct cgcgcctgcg tcgccatcgg ccgcgaaacc aggcgcccgt
     3781 cgggacgccg cgtcgtgaac attgcgatct cgatcccgtc gatcaggtcg tagagttggg
     3841 tcgccttttg ctgggtgcct gacatttgca gcctccgttg ttgtgagccc gtgcgctgcg
     3901 cgcgcacgcg tccagcagcc tgcaagatcg gtgccgagag ttcgtcccac gcctcgcggc
     3961 gtgcaggcgc ctgtgatgct tatttcgcga ggcgctcgct gacgtacgtc gcgacgtcgc
     4021 ggatctgctc ggccgtgagc gtcccgccgc cgaatgccgg catcgcgttg cggccctcgc
     4081 tgacgacttg cagcacgacg gcgcggttct tcagcgcctg caagctcggc ccgccgccgt
     4141 ggccgccttc gccttgctcg ccatggcacg cgaggcaagc tgtcgtatag acggttgcgc
     4201 ccgcgtcggc attgccgggt ccggcgccgc caggcgcgaa cgtcatcagc gagcccgccg
     4261 gaggcacggg ctcgagcgca ccgtcgaggc cgaacagcca gaggctgtcg ccgcgcgcgg
     4321 agccggcgaa ttgattccct gcggaatagg cgacgacgta ctgcttgccg tcgtgctcga
     4381 acgaggtcgc cggcgaattc atgccggcgc ccgtctggaa ctcccagagc ttcatgccgg
     4441 tgctcgagtt cagcgccgtc agccggccgt cgttgcggcc gacgaacacg agcccgcctg
     4501 ccgtcgcgag cgagccgctg tagcacgggc tcggccagtg ctgctgccag acgagcttgt
     4561 tcgtacgcac gtcgacggcg gcgaatacgc cgagggacgt catgtggaag ccgccgatgc
     4621 cgccggcgat gaagcccgcg ccctcggcgg gccgctcggg cccgacctct tgcgacaaga
     4681 acgaccagat gcgatcgccg gcgcaaacga acagcaaccc actcgtcgca tcgtacgagc
     4741 tcggcggcca gttcacgccg ccgggagggc ccggtttcgc gagcacgggc gtcgtccaga
     4801 acggcgtgaa gatcttgccc tcgttgacga ggacggtgcc ttcaggtgcg atgtcgatct
     4861 gctgcggcac gaacgcgtcg ccgcgcggat agggctgagt cgccgccgtg ccttggcgcg
     4921 gctcctgcgg cactgcgcgc tcgtcgacgc cgacgagcgg cgtgccgttc gtgcgatcga
     4981 gcacatagac ccaacctgt
//
LOCUS       DATLEE010000676         3574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10957058, whole genome shotgun sequence.
ACCESSION   DATLEE010000676 DATLEE010000000
VERSION     DATLEE010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10957058"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VM692_14790"
     CDS             complement(<1..740)
                     /locus_tag="VM692_14790"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-phosphate galactose phosphotransferase"
                     /protein_id="HUQ53491.1"
                     /translation="MAAAAVHIAAFVRFTGDLPPETEGALLWLRAVVFGAVVLIALTA
                     MGLYQIRQRLTIEGVLVRIGLGLGVAAMGLAVIYFAIPILRLWRGWWVLSFVVTLLLL
                     ICSRIVFAKAVDQSAFRRRVVVYGAGKRALTLLELRRRSDQRGFKLLAFLPVPNEPLL
                     VQDERVDLSGVGLLEFVRKHEAEEVVIAMDDRRHNFPIRELLDCKFAGIEVVDIFSFL
                     ERESGKVKIDLLAPSLMIFSEGFTQRTSR"
     gene            complement(882..2219)
                     /locus_tag="VM692_14795"
     CDS             complement(882..2219)
                     /locus_tag="VM692_14795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HUQ53492.1"
                     /translation="MRVTIFGTGYVGLVTGACLADSGNHVLCVDIDAAKIARLQAGEI
                     PIYEPGLEALVKASRAAGRLEFTTDTAAGVAHGVFQFICVGTPPDESGSADLQHVLAV
                     ARSIGENIKEYRVIVDKSTVPVGTADLVKAEVQRALDARKATAPFDVVSNPEFLKEGA
                     AVGDFMKPDRVIIGTSDPRTAELLRTLYEPFNRNRDRLIVMDVRSAELTKYAANAMLA
                     TKISFMNELANVAERVGADIEHVRVGIGSDPRIGYEFIYPGCGFGGSCFPKDVRALAS
                     SAADEGYEPALLRAVEAVNDRQKTVLFRKINDYFGGNLRGKTIALWGLAFKPNTNDMR
                     EASSRVLMEALWNAGARVQAYDPVASDEVQRIYGDREDLLLCNRAERALEGADVLAIV
                     TEWREFRSPDFDLIKKTLREPAVFDGRNLYDPDLVHQRGLRYFAIGRGDSLVA"
     gene            2481..2555
                     /locus_tag="VM692_14800"
     tRNA            2481..2555
                     /locus_tag="VM692_14800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2513..2515,aa:Val,seq:cac)
     gene            complement(2582..3184)
                     /locus_tag="VM692_14805"
     CDS             complement(2582..3184)
                     /locus_tag="VM692_14805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HUQ53493.1"
                     /translation="MTTSDGAVATVSDGGVGDLAAQAGTVVLLSPLGAAWSVNVTTGL
                     SKPTIGSADHPLLDLNSVNVSSVGGPSWIEIELTDTGFTEQTGAGFFSAIGGTTSGSV
                     SFKTYYDADNNAFGKGTELASLDSIGPAFAATAGGQFMSLTPFSLTLLVRVAHVGAQV
                     SSFDATVAVPEPGTLFLFGTGLVGLAGAFMRRGARKQTLA"
BASE COUNT          730 a         1157 c         1080 g          607 t
ORIGIN      
        1 cgcgacgtgc gctgggtaaa accttcggaa aaaatcatca acgatggggc gagtagatcg
       61 atcttgacct tgccggactc gcgctcgaga aacgagaaga tgtcgacgac ttcaatgccc
      121 gcaaacttgc aatccagcag ctcgcgaatg gggaagttgt ggcgccgatc atccatcgca
      181 atcactacct cctccgcctc atgcttgcgc acgaactcga gcagaccgac tcccgagagg
      241 tcgacgcgct cgtcctgcac gagcagcggc tcgttcggca cgggtaagaa tgcgagcagc
      301 ttgaaacccc gttgatcgct tcgccggcgc agctcgagca gcgtcagcgc acgcttgccc
      361 gccccataga caaccacacg ccgccggaaa gcactttggt ccacggcttt cgcgaagacg
      421 atgcggctac agatcaaaag tagcagcgtc acgacgaaac tgagaaccca ccaaccacgc
      481 cataggcgca ggataggaat ggcgaaatag atgaccgcga gacccatcgc ggctacgcca
      541 agcccgagcc cgatgcgaac gagcacgcct tcgatggtca atcgttggcg aatttgataa
      601 aggcccatcg ccgtgagggc gatcaggacg acggccccga ataccacggc gcgcaaccac
      661 agcagcgccc cctccgtttc aggcgggaga tcgccggtga agcggacgaa cgcggcgatg
      721 tgcactgcgg cagcggccaa cacgaactcg gcgacggcga gcagggctat ttttgagctt
      781 atgcgcaacc gagcgctccg ggcgctccgg gcgcggcggc ggcagctcga agcgcgtctt
      841 tcacgccggt gatggacgaa gtcgggccgt gctgcgaggg cctaggcgac aagcgaatcg
      901 ccgcggccga tcgcgaaata tcgcaagccg cgttggtgca ccaggtcggg atcgtagagg
      961 ttgcgtccgt cgaacacggc cggctcccgc agcgttttct tgatcaggtc gaagtcgggg
     1021 ctgcgaaact cccgccactc cgtcacgatc gcaaggacgt ccgcgccttc gagcgcccgc
     1081 tccgcacggt tgcacagcag caggtcctcg cggtcgccgt agattcgctg cacttcgtcg
     1141 ctcgcgacgg ggtcgtaggc ttggacgcgt gcgccggcgt tccaaagcgc ttccatcaac
     1201 acgcggctcg acgcctcgcg catgtcgtta gtgttcggct tgaatgccag cccccagagc
     1261 gcaatggtct tgccgcggag gttacccccg aagtagtcgt tgatcttcct gaacagcacg
     1321 gtcttctggc gatcgttgac ggcttcgacc gcgcgcagca gcgccggctc gtatccctca
     1381 tcggccgccg acgacgccag cgcacgcacg tccttcggaa aacacgaacc gccgaaccca
     1441 cagccgggat agatgaactc gtagccgata cgcggatcgg agccgattcc gacgcgcacg
     1501 tgctcgatgt ccgcgccgac gcgctcggcg acgttcgcga gctcgttcat gaagctgatc
     1561 ttcgtggcga gcattgcgtt cgccgcatac ttcgtgagct cagccgaacg cacgtccatg
     1621 acgatcaggc gatcgcggtt gcgattgaac ggctcgtaca acgttcgcag cagctcagct
     1681 gtacgcggat cgctcgtgcc gatgatcacg cggtcgggct tcatgaaatc cccgacggcg
     1741 gcgccttcct tcaagaactc gggattcgaa acgacgtcga acggcgctgt cgctttgcgc
     1801 gcatccagag cgcgctgaac ctctgctttc acgagatcgg ccgttccgac gggcaccgtc
     1861 gacttatcga cgatgactcg gtactccttg atgttctcac cgatggatcg tgccacggcg
     1921 agaacgtgct ggaggtccgc cgagccgctc tcgtccggcg gtgtacctac gcaaatgaat
     1981 tggaacactc cgtgcgcgac gccggccgcc gtgtccgtcg tgaactccag gcgacccgct
     2041 gctcggctcg ctttgacgag cgcctcgaga ccgggctcgt agatcggtat ctccccggct
     2101 tggagtctcg cgatcttggc cgcgtcgata tcgacgcaga ggacgtggtt accgctgtcc
     2161 gcgaggcacg cgccagtgac cagccccacg taccccgtgc cgaaaatcgt gactcgcatc
     2221 gctcaactca ccccgtcgaa aagatggcta ccgaactgcg gcgcggccgg catgcgactg
     2281 caaccacacg cgcgcctccc cgacggactt gccgggtatt gcggcgtctc ccaggaagag
     2341 aatgatacag ggactcgatg aagaagagct tgaagcacgg ctcgccgaat accccggcgg
     2401 cgcttcagtc gttgccccaa ccgacctcac agagtttgac cctgtcgcgg gtggcccact
     2461 acactcctac gcggttcgac gggcgattag ctcagcggga gagcgctccc ttcacacggg
     2521 agaggtcgca ggttcaatcc ctgcatcgcc caccaccctt acttcagccg cccgctcgcc
     2581 attaggcgag agtttgcttg cgagcaccgc gccgcatgaa ggcaccggct aaaccgacta
     2641 agccggttcc gaacaagaac agagtgcccg gctccggcac agcgacggtc gcgtcgaatg
     2701 agctgacttg cgcacccacg tgcgcgacac ggaccagaag tgtcagcgag aacggcgtca
     2761 gcgacatgaa ctgaccgccg gccgtcgccg cgaacgcggg gccgatcgag tcgaggctgg
     2821 cgagctcggt gcctttaccg aacgcgttgt tgtctgcgtc gtaatacgtt ttaaagctca
     2881 ccgacccact cgtggttccg ccaatcgcgg agaagaagcc agcgccggtt tgctcggtga
     2941 aaccagtatc ggtgagctcg atctcgatcc agctcgggcc gcccaccgag ctgacgttta
     3001 cggaattcag atccagcagc ggatggtccg cggaaccgat cgtcggcttc gatagccccg
     3061 tcgtcacgtt gacggaccac gccgcaccaa gcgggctcaa tagaactacg gtgcccgctt
     3121 gggcggccag gtcgccgacg cccccgtcgc tgacagttgc gacagcaccg tcgctagtcg
     3181 tcaaccgaag gatgggaatc gcgttggctt gcgtcgttgc gcaaacaccg agaacagcgg
     3241 cgaacaaaat ccttatcgat ccggtatgca tgagtaggtt ccttccaaag ctcgcaacag
     3301 gtaaagcagc ttttgtgcca tcagaacgct agaggaaaac ctgctttcac atgaacggct
     3361 tacgttggga cgctacgaag cgcgatacgc gagctgtaaa gaacgctgac gctcgcgctg
     3421 ctgtgcattc gacagaacgt cgcgcaaacc gtgacgcggt tcacactgct aacgagagcg
     3481 ggtgaggaca agacgagcgc cagatgcgag acgcacgcga gctcgatcgt cgacagtgca
     3541 acgacgagcc acgcaccgct cgtgaaaggt cgcg
//
LOCUS       DATLEE010000677         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8725684, whole genome shotgun sequence.
ACCESSION   DATLEE010000677 DATLEE010000000
VERSION     DATLEE010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8725684"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VM692_14810"
     CDS             complement(<1..674)
                     /locus_tag="VM692_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53494.1"
                     /translation="MLLVDTHVHMYDCFGVAEVFDSAAANFASAARSLRSGAEFDGVL
                     CLVESASERFVDGVRSQRLGRVWRGRRGYWELEDCCEPETLVFRCGHVRLAVVAGRQV
                     VTRERLEVLALGTTAPVPDGDSIDTTLEAVRAAGAAAVLPWGVGKWLGARGAIVDRIL
                     AEPKWNHVFLGDNGNRLRYGRDPARFASARRAGRSVLPGSDPLPLPGEERRVGGYGFA
                     IDVDLDP"
     gene            complement(691..1680)
                     /locus_tag="VM692_14815"
     CDS             complement(691..1680)
                     /locus_tag="VM692_14815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ53495.1"
                     /translation="MIDIDVIVPVGARTDDLVPLHHERREALRDAGYNATFTYVIDGE
                     LPQVRDTLASLARGGDDACVIQLSRRFGETAAVLAGFANTKTELVMILPAFEQVETAS
                     LGRVIEALADADLVTVRRFPRCDSGWRRAQTQVFEAFIKGVGSSRFRDPGCTVHALRR
                     TVLEETQLYGEQHGFLPLLAANVGFKVVEIDLPQARTDAARKVQRPRSYIHRLVDILS
                     VFFLTRFTRRPLRFFGPAGAACTALGALGLVLVVVQRLFLGMPLAERPALILSSLLVA
                     VGLQVLAIGLIGELIVFIHARSMRQYRVREIIEAGGDARRSKPARLAPVGAAE"
     gene            complement(1677..>2359)
                     /locus_tag="VM692_14820"
     CDS             complement(1677..>2359)
                     /locus_tag="VM692_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53496.1"
                     /translation="QNDPRDIPMLVDTLEEDYDLVAGWRMKRQDKLVTRKIPSRIANW
                     LIGKITGIPIRDNGCSLKAYRASVMRHVPLYSEMHRFIPAMSSIVGARIIQVPVRHHA
                     RQFGSSKYGLSRIYKVLLDLISIKLVVAYANQPLVWFLKMALAPAVLAAAFTTAGVVR
                     AADGEYSLPLFGSGLILALAVGFLIALGSLGELAKHLARRDVVPLVTASAKYSKPLTI
                     QEDPGAPP"
BASE COUNT          427 a          795 c          811 g          326 t
ORIGIN      
        1 ggatcgaggt cgacgtcgat cgcgaagcca tagccgccga cgcgccgttc ctcgccgggc
       61 aacggcagcg gatccgagcc cggcagcacc gaccggccgg cgcggcgcgc cgacgcgaac
      121 cgcgccgggt cgcgaccgta gcgcaaccga ttgccgttgt cgccgagaaa cacatggttc
      181 cacttcggct cggccaggat gcgatcgacg atggcgccgc gcgcgccgag ccacttcccc
      241 acgccccaag gcaacacggc cgccgcgccg gcggcgcgca cggcctccaa cgtcgtgtcg
      301 atcgagtcgc cgtcgggcac cggcgccgtc gtgccgagtg cgagcacctc gagccgttcg
      361 cgcgtcacga cttgacggcc cgcgacaacg gcgagtcgta cgtggccgca gcggaaaacg
      421 agcgtctcgg gctcacagca gtcctcgagc tcccagtagc cgcgcctgcc gcgccaaacc
      481 cggccgagac gttggctgcg tacgccgtcg acgaagcgct cgctggcgct ttcgacgagg
      541 cataggacgc cgtcgaactc cgccccgctg cgtaatgaac gagccgcgct cgcaaaattg
      601 gcggccgcag agtcgaaaac ctcggcgacg ccgaagcagt catacatgtg cacgtgggta
      661 tcgacgagca gcacagtggc tcgagagcga tcactcggcc gcgccgacgg gcgccaagcg
      721 cgcgggcttc gaacggcgcg catcgccgcc ggcttcgatg atctcgcgaa cgcggtactg
      781 gcgcatcgag cgcgcgtgga tgaacacgat gagctcgccg atgagcccga tcgcgagcac
      841 ctgtaacccg accgccacga gcagcgagct caaaatcagc gccggccgct ccgcgagcgg
      901 catgccgagg aacagccgtt gcacgacgac gagcacgagg ccgagcgcgc cgagcgccgt
      961 gcaggcggcg ccggcgggtc cgaagaagcg cagcggccgc cgcgtgaacc gcgtcaggaa
     1021 gaacaccgac aggatatcga cgagccggtg gatgtagctg cgcggccgct gaaccttgcg
     1081 cgcggcgtcc gtgcgcgcct gcggcagatc gatctcgacg accttgaaac cgacgttggc
     1141 agcgagcagc ggtaggaaac cgtgctgctc gccgtacagc tgcgtctcct cgagcacggt
     1201 gcgccgcagc gcgtggacgg tgcagcccgg atcacggaag cgggagctgc cgacgccttt
     1261 gatgaacgcc tcgaatactt gcgtctgcgc tcgccgccag ccggagtcgc agcgcggaaa
     1321 gcgccgcacc gtgaccaagt cggcgtcggc gagcgcctcg atgacgcggc cgagcgatgc
     1381 ggtctcgacc tgctcaaacg ccggcaagat catgacgagc tcggtcttcg tgttggcgaa
     1441 gccggccagc accgcggcag tctcgccgaa gcggcgcgag agctgaatca cgcaggcatc
     1501 gtcgccgccg cgtgcgagcg acgccaacgt gtcgcgcact tgcggcaact cgccgtcgat
     1561 gacgtaggtg aaggtagcgt tgtacccggc gtcgcgtagc gcctcgcggc gctcgtggtg
     1621 cagcggcacg agatcgtcgg tcctcgcgcc tacggggacg atcacgtcga tatcgatcat
     1681 ggcggagctc ctggatcttc ttgaatagtc agcggcttcg aatacttggc ggacgccgtc
     1741 acgagcggca cgacgtcgcg gcgtgcgaga tgtttcgcga gctcgccgag cgagccgagc
     1801 gcgatcagaa acccgaccgc gagcgcgagg atcaggcccg agccgaacag cggcagcgag
     1861 tactcgccgt cggcggcccg cacgacgccg gccgtcgtga acgccgcggc gagcaccgcg
     1921 ggcgccagcg ccatcttcag gaaccagacg agcggctggt tcgcatacgc gacgacgagc
     1981 ttgatcgaaa tcaggtcgag cagcactttg tagatgcgcg acaagccgta cttcgagctg
     2041 ccgaactggc gcgcgtgatg ccgcacggga acctggatga tgcgcgcgcc gacgatgctg
     2101 ctcatcgccg gaatgaagcg gtgcatctcc gagtacagcg gcacatggcg catgacggag
     2161 gcgcggtacg ccttcagcga gcagccattg tcgcggatcg gaatgcctgt gatcttgccg
     2221 atcaaccaat tcgcgatgcg cgaggggatc ttgcgggtca cgagcttgtc ctggcgcttc
     2281 atacgccagc cggcgacgag gtcgtaatct tcctcgagcg tatcgacgag catcgggatg
     2341 tcgcgcgggt cgttctgca
//
LOCUS       DATLEE010000678         3213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7239482, whole genome shotgun sequence.
ACCESSION   DATLEE010000678 DATLEE010000000
VERSION     DATLEE010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7239482"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /gene="pstC"
                     /locus_tag="VM692_14825"
     CDS             <1..1123
                     /gene="pstC"
                     /locus_tag="VM692_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HUQ53497.1"
                     /translation="DQLGLIYNDIRNLVEGNVVTAAPSPEIAEAAKQYTAQRELSRNL
                     ATAAVLVLGVAVFGWAYRRIQPALRARNRVERIIELLFIAASCVAILTTIGIFLSVFV
                     EALRFFRQVSLLDFLFGLTWSPQTAIRADQVGSAGAFGAVPLFVGTLLISGLAMLVAV
                     PVGLMSAIYLSEYATRRLRAYAKPLLEILAGIPTVVYGYFAALTVAPLLRGLGDSIGL
                     DVASESALGAGLVMGIMIIPFVSSLSDDVITAVPQALRDGSYALGATRSETIRNVVFP
                     AALPGIVGAVLLAVSRAIGETMIVVMAAGLAANLTANPLEAVTTVTVQIVMLLTGDQE
                     FDSAKTLAAFALGLVLFIVTLALNVVALRVVRRYREQYE"
     gene            1116..2405
                     /gene="pstA"
                     /locus_tag="VM692_14830"
     CDS             1116..2405
                     /gene="pstA"
                     /locus_tag="VM692_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HUQ53498.1"
                     /translation="MSSRSSLVKEAVARGIARRYRNELIFRSLGLMALLVGLGFLAFF
                     FYTLIGNGYTAFRQTRVQLDVTLDAAIIDPDATRDPDTLRGADYQRIARNALGALFPD
                     VTSRADLRALYALLSPGAGLELQRLVVDEPNVIGETRTMWLLADDDVDVLVKGHSPRD
                     VPEADRRVDDRQLGWLTALEQQGRVDLQFNRYFFTRGDSRDPELAGIWGATVGSFFML
                     LVTLALSFPLGVATALYLEEFAPRNRWTDLIEVNINNLAAVPSIVFGLLGLALFINVF
                     GLPRSAPVVGGLVLSLMTLPVIIIASRAALTAVPPSIREAALGLGASKMQMVLHHVLP
                     LAMPGMLTGTIIGMARALGESAPLLMIGMVAFIVDVPDSPLSPATALPVQVYLWADSP
                     ERAFVERTSAAIVVLLMFLLVMNAAAILLRKRFERRY"
     gene            2595..>3213
                     /locus_tag="VM692_14835"
     CDS             2595..>3213
                     /locus_tag="VM692_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HUQ53499.1"
                     /translation="MQARQVDVFYGDNHAIQKVNLDIGRNEVIALIGPSGCGKSTFIR
                     CLNRMNDTIELARITGEITLDGENVYARHMDVVMLRARVGMVFQKPNPFPKSVYDNVA
                     YGPRIHGLARDRGELDEIVQTSLVRAGLWTEVKDRLEEPGTGLSGGQQQRLCIARTIA
                     VNPDVILMDEPCSALDPIATARIEDLIDELKQNYAIAIVTHSMQQA"
BASE COUNT          505 a         1041 c         1046 g          621 t
ORIGIN      
        1 ggatcagctc gggctcatct ataacgacat ccgcaatctg gtggaaggta acgtagtcac
       61 ggcggcgccg agccccgaga ttgcggaggc cgcaaagcag tacacggcac agcgagagtt
      121 gagccgcaac ctcgccacgg cggcggttct cgtgctcggc gtcgcggtct ttggttgggc
      181 gtatcgacgc atccagccgg cgttgcgcgc acgtaatcgc gtcgagcgca tcatcgagct
      241 gctgttcatc gcggcctcgt gcgtcgcgat cctgacgacg atcggcatct tcttgtccgt
      301 gttcgtcgag gcactgcgct tctttcggca agtatcgttg ctcgatttcc tgttcggctt
      361 gacgtggagc ccgcagaccg cgatccgcgc cgatcaagta ggctcggccg gcgcattcgg
      421 tgccgtgccg ttgttcgtcg ggacgctgct gatctcaggt ctcgcgatgt tggtggccgt
      481 gcccgtcggg ctgatgtcgg cgatctatct gtccgagtac gcgacgcggc gcttgcgggc
      541 ttacgcgaag ccgctgctcg agatcctcgc gggtattccg acagtcgtat acggctactt
      601 cgccgcgctg accgtcgcgc cgctgctgcg cggcctcggc gacagtatcg ggctcgacgt
      661 cgcgtcggag agcgcgctcg gcgctggcct cgtcatgggc atcatgatca tcccattcgt
      721 ctcgtcgttg tccgacgacg tcatcacggc tgtgccgcaa gcgttgcgcg acggctcgta
      781 cgccctggga gccacgcgtt ccgagacgat ccgcaacgtc gtgttcccgg ccgcgttgcc
      841 gggcatcgtc ggcgccgtgt tgttggcggt ctcgcgcgcg atcggcgaga cgatgatcgt
      901 cgtcatggcg gcggggcttg ccgcgaatct cacggcgaac ccgctcgaag ccgtcacgac
      961 ggtgacggtg cagatcgtca tgctgctaac gggcgaccaa gagttcgaca gtgccaagac
     1021 gctcgcggcg ttcgcgctcg gcctcgtgtt gttcatcgtt acgctggcgc tgaacgtcgt
     1081 cgcgttgcgg gtcgtgcggc gctaccggga acaatatgag tagccgctcg agcttggtca
     1141 aagaagccgt cgcacgaggc attgcacgcc gttaccgcaa cgagctcatc tttcgctcgc
     1201 tgggcctcat ggcgctgcta gtcggcctcg gcttcctcgc attcttcttc tacacgctga
     1261 tcggcaacgg ctacacggcg ttccgccaaa cacgcgttca gctcgacgtg acgctcgacg
     1321 cggcgatcat cgatcccgac gcgacacgcg atcccgatac gttgcgcgga gccgactatc
     1381 agcgcatcgc ccgcaatgcg ctcggcgcac tgttccccga cgtcacgagc cgcgccgatt
     1441 tgcgcgcgct gtacgcgctg ctgagcccgg gtgcggggct cgagctgcaa cgtctcgtcg
     1501 tcgacgagcc gaacgtgatc ggcgaaacgc gcacgatgtg gctgctcgcc gacgacgacg
     1561 tcgacgtgct cgtcaaaggg cattcgccac gcgacgttcc cgaagcggat cgccgagtcg
     1621 acgaccgcca gctcggctgg ctcacggcgc tcgagcagca ggggcgcgtc gacctgcagt
     1681 tcaatcgcta cttcttcacg cgcggcgact cgcgcgatcc ggagctcgcc ggcatctggg
     1741 gcgcgacggt cggctccttc ttcatgttgc tcgtaacgct cgcgttgtcg ttcccgctcg
     1801 gcgtcgcgac ggccctctat ctcgaggagt tcgcgccgcg caatcgctgg accgacctca
     1861 tcgaggtcaa catcaacaat ctcgcggcgg tgccgtcgat cgtgttcggc ttgctcgggc
     1921 tcgcgttgtt catcaacgtg ttcgggctac cgcgctcggc gcccgtcgtc ggcggcctcg
     1981 tgttgagcct catgacgctg ccggtgatca tcatcgcgtc gcgcgcggcg ctgaccgcgg
     2041 tgccgccgtc gatacgcgaa gcggcgctcg ggctcggcgc ctcgaagatg cagatggtgc
     2101 tgcatcatgt gctgccgctc gcaatgcccg gcatgttgac gggcacgatc atcggcatgg
     2161 cgcgagcgct cggcgagtct gcgccgctgc tgatgatcgg catggtggcg ttcatcgtcg
     2221 acgtgcccga ctcgccgctg agcccggcga cggcattgcc cgtgcaggtt tatctctggg
     2281 ccgacagccc tgagcgcgcg ttcgtcgagc gaacgtccgc cgcgatcgtc gtgctgctga
     2341 tgttcctgct cgtgatgaat gcggccgcga tcttgttgcg gaagcgtttc gagcggcgct
     2401 actaggccta caaagggcgt cgccgcgcat agttggggta cgatgtgagc ctagccgcag
     2461 aaacaccgaa caactctgtg ccagaggtcg caacgaaagc ggcggaaacg ccgtcggttc
     2521 aagccgtgtc gccgaaagcc gcggccgacg agggctatcg cactgtcggt gacttcacgt
     2581 cggcgaatcc cgtcatgcag gcgcggcaag tcgacgtgtt ctacggcgac aatcatgcga
     2641 ttcagaaggt caatctcgac atcggccgca acgaggtcat cgcgctgatc ggcccgtcgg
     2701 gttgcggcaa gtcgacgttc atccgttgtt tgaatcgcat gaacgacacg atcgagttgg
     2761 cgcgcatcac gggcgaaata acgctcgacg gcgagaacgt ctatgcgcgc cacatggacg
     2821 tcgtcatgct gcgcgcgcgc gtcggcatgg tcttccagaa gccgaatccg tttccgaagt
     2881 ccgtctacga caacgttgcc tacggcccgc gcattcacgg gctcgcgcgc gatcgcggcg
     2941 agctcgacga gatcgtacag acgagcctcg tgcgcgccgg gctatggacc gaggtcaagg
     3001 atcggctcga ggagcccggc acgggtctat ccggcggtca gcagcagcgg ctttgcatcg
     3061 cgcgcacgat cgccgtgaac cccgacgtca tcctcatgga cgagccgtgc tcggcgctcg
     3121 acccgattgc gacggcgcgt atcgaggatc tcatcgacga gctgaagcaa aattacgcga
     3181 ttgcgatcgt cacgcactcg atgcagcagg ctg
//
LOCUS       DATLEE010000679         3217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7247808, whole genome shotgun sequence.
ACCESSION   DATLEE010000679 DATLEE010000000
VERSION     DATLEE010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7247808"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VM692_14840"
     CDS             complement(<1..190)
                     /locus_tag="VM692_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53500.1"
                     /translation="MNTDSLFKQLDAPPGGAERFARRLGDATAAPAVAPRWVQFALAG
                     ATAAAAALVVAVVLSRAPD"
     gene            complement(180..689)
                     /locus_tag="VM692_14845"
     CDS             complement(180..689)
                     /locus_tag="VM692_14845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUQ53501.1"
                     /translation="MTSVDDTQWIDAYRRLEKPLYNVVYRVLWDAAESQDIVQDAFLR
                     CWRRKHDVRADGFTALLYRTALNLASNRRRRMRLWRMVGVAAIAELVDESEGSETPIS
                     PVVRAAFEALPDALKHVVLLTEIAGMTYPEVAATLAIAEGTVGSRRSRALALLRERLE
                     VQGVVSHEY"
     gene            complement(717..1463)
                     /locus_tag="VM692_14850"
     CDS             complement(717..1463)
                     /locus_tag="VM692_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53502.1"
                     /translation="MRTRLTAVGLSLVLSSNAWADLRIFDVGVQHQQEIYVALGNVLN
                     PATVGGGNPYGTVQQLPNGQLLVNAPPQVLQQVEQVVTAIRERPVAAAPRVSLRYWAV
                     LGTQAQGAAANAIGSAPPPALNAVLTELKRLNGELTFRVIGTAAVTSDSGQKGEVNAS
                     TMSVSQQTQVQGDTLNAYMRINVQSIRAPESNAPRVDVQGAAIVPTFPIGELELRTSL
                     KRGEFVVLGESQAQGNGVDGPVFYIVHWAE"
     gene            complement(1585..2442)
                     /locus_tag="VM692_14855"
     CDS             complement(1585..2442)
                     /locus_tag="VM692_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HUQ53503.1"
                     /translation="MTAELRSFIERYPRLFVLTGAGCSTDSGIPDYRDDDGEWKRGKP
                     VMLEDFLHDERWRKRYWARSLVGWQRMQAAEPNDAHRALAALEADGRVAQLVTQNVDG
                     LHQAAGSRNVIDLHGRVDVVRCISCERQAPRALLQAELVRRNAAFVDLAATAAPDGDA
                     ELELVDFAKFDIPSCDACGGLLKPDVVFFGERVPPDRVHAAFAALERSDAMLVVGSSL
                     MVYSGYRFARAMADLGKPVAAVNLGRTRADELLTLKVSARCAEALQFLLPGRPRPPLP
                     PPEPLRARP"
     gene            complement(2439..3056)
                     /locus_tag="VM692_14860"
     CDS             complement(2439..3056)
                     /locus_tag="VM692_14860"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HUQ53504.1"
                     /translation="MAVQPKMLYPLFSTPVYVNNVGDFPRPDLKRLEYTTGDYRFATS
                     VDKRVLHRPEFKGMHDIVLREVESYTREVLAVNRSIEFYVTDSWINIYRPGEGAGPHT
                     HNNSLISGVLYLKVNEASGDLVFQRDIHSLVPFPPALDLDMDFFNIYNCKSWSHKPKV
                     NDICLFPSVVMHAADPNRSNEERWCLAFNVFVRGNIGALHKLEIR"
BASE COUNT          539 a         1048 c         1093 g          537 t
ORIGIN      
        1 tgtccggtgc gcgcgacagg acgacggcaa cgacgagcgc ggcggctgct gccgtagcac
       61 cggccagcgc aaactgaacc caccgcggcg caaccgcggg tgcggcagtg gcgtcgccaa
      121 gccgccgcgc gaagcgctcg gcgccgcccg gcggcgcgtc gagttgctta aatagcgaat
      181 cagtattcat gcgagaccac tccttggacc tcgagccgct cgcgcaacag cgcgagcgcc
      241 cgtgaccttc gcgagccgac ggtgccttcg gcgatcgcga gcgtagcggc gacttcagga
      301 tacgtcatgc ccgcgatctc ggtcagcagc acgacgtgct tcagcgcgtc gggcagggct
      361 tcgaacgctg cccggacgac gggtgagatc ggcgtctcgg agccctcgct ctcgtcgacg
      421 agctcggcga tcgctgcgac gccgaccatg cgccagagcc gcatacggcg ccggcggttg
      481 ctcgcgagat tcaatgccgt gcgatacagc agcgccgtga aaccgtcggc gcgcacgtcg
      541 tgcttgcgcc gccaacagcg caagaacgcg tcctgcacga tgtcctggct ctccgccgca
      601 tcccacagca cgcgatagac gacgttgtag agcggcttct cgagccgccg atacgcgtcg
      661 atccattgcg tgtcgtcgac gctcgtcatc tcgatgcgtt ccgagagcga gcgctgctat
      721 tccgcccagt gcacgatgta gaacacgggg ccgtcgacgc cgttgccttg cgcttggctt
      781 tcgccgagca cgacaaattc gccgcgtttc agcgaagtgc gaagctcgag ctcgccgatc
      841 ggaaaggtcg gcacgatggc tgcgccctgg acgtcgacgc ggggcgcgtt gctttcaggg
      901 gcgcgaatgc tctgaacgtt gatgcgcatg tacgcattca acgtatcccc ttgcacttgg
      961 gtctgttggc tcacgctcat cgtgctggcg ttgacttcgc ctttctgccc ggagtccgac
     1021 gtgaccgctg ccgtgccaat gacgcggaac gtcagctccc cgttgagccg cttgagctcg
     1081 gtaagaacag cgtttagcgc aggtggcggc gcactaccga tcgcgttggc tgccgctcct
     1141 tgagcctgcg tgccgagcac ggcccaatac cgcagcgaca ctcgcggcgc cgcggcgacg
     1201 ggccgctcgc ggatcgccgt gaccacctgc tcgacttgct gcagcacctg cggcggcgcg
     1261 ttgacgagga gctggccgtt cggcagttgc tgaacggtcc cgtaggggtt gccgccaccg
     1321 acggtggccg gattcaagac gttgccgagc gcgacgtaga tttcctgctg atgctggacg
     1381 ccgacgtcga agattcgcag gtcggcccag gcattgctcg aaagcacgag cgacaggcca
     1441 acggcagtaa gacgggtgcg catggcgggt ctccttggcg tgtgacacct atatatacac
     1501 gcgagccggc caatcccttc ccgtgacggg ctacgtcggc gtactgggtc tactgttcga
     1561 gcgatgccgc agaagacggc cggctcaggg ccgcgcgcgc agcggctccg gcggcggcag
     1621 cggaggccgc gggcgacccg ggagcagaaa ctgcagggct tccgcgcagc gcgcgctgac
     1681 cttcagcgtc agcagctcgt cggcccgcgt gcggccgaga ttgacggcgg cgacgggctt
     1741 gccgagatcc gccatcgctc tcgcgaaacg gtagcccgag tagaccatca gcgacgagcc
     1801 gacgacgagc atcgcgtcgg agcgctcgag cgccgcgaac gcggcgtgca cgcggtctgg
     1861 cggaacgcgc tcgccgaaga acacgacgtc gggcttcaac aggccgccgc aagcatcgca
     1921 actcgggatg tcgaacttcg cgaagtcgac aagctcgagc tccgcgtcgc cgtccggcgc
     1981 cgcggtggcg gcgaggtcga cgaacgcggc attacggcgc acgagctccg cttgcagcag
     2041 cgcgcgcggt gcttgccgct cgcagctgat gcagcgcacg acgtcgacgc ggccatgcag
     2101 gtcgatgacg ttgcggctgc ccgcagcctg gtgcaagccg tcgacgttct gcgtgacgag
     2161 ctgggcgacg cggccgtcgg cctcgagcgc cgcgagcgcc cgatgtgcat cgttgggctc
     2221 ggcggcctgc atgcgctgcc agccgacgag gctgcgggcc caatagcgct tccgccagcg
     2281 ctcgtcgtgt aagaagtcct cgagcatgac gggcttgccg cgcttccatt cgccgtcgtc
     2341 gtcgcggtag tcgggaatgc cggagtccgt gctacagccg gcgcccgtca gcacgaatag
     2401 ccggggatag cgctcaatga aactgcgcag ctccgccgtc atcgaatctc gagcttgtgc
     2461 agcgccccga tgttgccgcg cacgaagacg ttgaatgcca agcaccagcg ctcctcgttg
     2521 gaccggttcg gatcggcggc gtgcatgacc acggacggga acaagcagat gtcgttgacc
     2581 ttcggcttgt ggctccagct cttgcagttg tagatgttga agaagtccat gtcgagatcg
     2641 agcgcgggcg ggaacggcac gaggctatgg atgtcgcgct ggaacacgag atcgccgctc
     2701 gcttcgttga ccttgaggta gagcactccg gagatcagcg agttgttgtg cgtgtgcggg
     2761 ccggccccct cgccgggccg atagatgttg atccacgagt cggtgacata gaattcgatg
     2821 ctgcggttca cggccagcac ttcgcgcgtg tacgactcga cttcgcgcag cacgatgtcg
     2881 tgcatgccct tgaactcggg gcggtgcagg acgcgtttgt cgacggacgt cgcaaaacga
     2941 tagtcgcccg tcgtgtactc gagtcgcttg aggtcgggcc gcggaaaatc gccgacgttg
     3001 ttcacgtaga ccggagtcga gaacaacgga tacagcatct tgggctgcac ggccatgacg
     3061 tcagcccgcc aagaacacgc gcagctcggc gagcgtttcc tgtggatgcg attcctggaa
     3121 tgaatggccg cccggcatct ggcggcccga gacgttggtg ccgcgctcgc cccagaccgc
     3181 gagcgcgtcg tagcggccgg cgacggagcc acgctcg
//
LOCUS       DATLEE010000680         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6414146, whole genome shotgun sequence.
ACCESSION   DATLEE010000680 DATLEE010000000
VERSION     DATLEE010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6414146"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VM692_14865"
     CDS             <1..1028
                     /locus_tag="VM692_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HUQ53505.1"
                     /translation="QWVRANIAEFGGDAGNVTVFGQSGGGRKVATLLAMPSAKGLFHR
                     AIIESGATIQLVEPEQAARVARELLSELKLEPRQWRELQTVPLATLMSAYFSTVRRMS
                     VDQMTMGFSPTMDGTAVAQHPFHPNAAAVSADVPLIIGSTRTELTSSAAAAEFSLDDA
                     GMRARVGALLGDSANRVIDVYARLNPRATPSELWFLIASDERYGAPVMKIAERRAALG
                     RGPVYLYYFRWETPLEGGRYMAPHTIEIPFAFHNLEASPWTRGVAGAAALADQVSDAW
                     LAFARSGDPNTSGVPAWPKFDASRRATMVFDDPWKSPKGDTASAAADDPLREQRLAMF
                     AAKGLTT"
     gene            1025..>2408
                     /locus_tag="VM692_14870"
     CDS             1025..>2408
                     /locus_tag="VM692_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HUQ53506.1"
                     /translation="MSAARWRRRALLKAGALAVGASLLPPARSRAQEIGDVMDALSRY
                     MAAARERALPEQVVEHVKHHVLDTLAAMVSGTELKPGQAALRFAATQTHGGAATVIGA
                     DFTLDAGAAALVNGTCAHADETDDSHGPSQSHPGASVVAAALALGESRGIDGARFMRA
                     VALGYDVGPRVTMALGGVEFRNETRRSTHAFAGTFGASAAAACAAGLDARQMRWLLDY
                     ASQQAAGYAVWGRDTEHVEKAFVFGGMPARAGVTAAELVAAGWSGVDDVFSGEDNFFA
                     VNAPNGDPGVLVAELGARYEVGNTDIKKWSVGTPIQAPLDALEALRKQRPFRAADVTE
                     VVVRLAPTVGAVVDNRDIPDICLQHMVAVMLLDGTASFAAAHDAARMQDAAVLRERAK
                     VRYVPDAQLAALLPARVAVVEVALRDGTRINERVEAVRGTVRNPMSREEVVAKARDLV
                     EPVFGVATAGR"
BASE COUNT          348 a          843 c          881 g          336 t
ORIGIN      
        1 tgcagtgggt gcgggcgaac atcgcggaat tcggcggcga cgccggcaac gtcaccgtgt
       61 tcggccagtc gggcggcggc cgcaaggtcg cgacgttgct cgcgatgccg tcggccaaag
      121 ggctctttca ccgcgcgatc atcgagagcg gcgcgacgat ccaactcgtt gagcccgaac
      181 aagcggcgcg cgtcgcgcgc gagctcctga gcgagctgaa gctcgagccg cggcagtggc
      241 gcgaattgca gacggtgccg ctcgcgacgc tgatgagcgc gtacttctcg acggtgcgcc
      301 gcatgagcgt cgatcagatg acgatgggct tctcgccgac gatggacggc acggccgtcg
      361 cgcagcatcc gttccatccg aacgcggccg cggtctcggc cgacgtgccg ctgatcatcg
      421 gctcgacgcg cacggagctg acgtcgagtg ccgcggctgc cgagttctcg ctcgacgacg
      481 ccggcatgcg cgcgcgagtc ggcgcgctgc tcggcgactc cgcgaaccgc gtcatcgacg
      541 tgtacgcgcg cttgaacccg cgcgcgacgc cgtcggagct ctggtttctg atcgcgagcg
      601 acgagcgcta cggcgcgccc gtcatgaaga tcgcggagcg gcgggccgcg ctcggccggg
      661 gccccgtcta tctctattac ttccgctggg agacgccgct cgagggcggg cgctacatgg
      721 cgccgcacac gatcgagatt ccgttcgcgt tccacaacct cgaggcgtcg ccgtggacgc
      781 gcggcgtcgc gggcgccgcg gcgttggccg accaagtcag cgacgcatgg ctcgcgttcg
      841 cgcgcagtgg cgatccgaac acgagcggcg tccccgcgtg gccgaagttc gacgcgagcc
      901 gccgcgcgac catggtgttc gacgaccctt ggaagtcccc gaagggggac acagcgagcg
      961 ccgcggccga cgatccgctg cgcgagcagc gtctcgcgat gttcgcggcg aaaggcctca
     1021 cgacttgagc gccgcgcgct ggcggcgccg cgcgctgctg aaggccggag cgctcgcggt
     1081 cggcgcgagc ttgttgccgc cggcgcgtag ccgcgcgcaa gaaatcggcg acgtcatgga
     1141 cgcgctgagc cgttacatgg ccgcggcgcg cgagcgcgcg ttgcccgagc aggtcgtcga
     1201 gcacgtcaag catcacgtgc tcgacacgct ggccgcgatg gtctccggaa cggagctcaa
     1261 gccagggcag gcggcgctgc gcttcgccgc gacgcaaacg cacggcggtg cggcgaccgt
     1321 gatcggcgcc gacttcacgc tcgacgcggg cgctgccgcg ctcgtcaacg gcacctgcgc
     1381 acacgccgac gagaccgacg actcgcacgg gccatcgcaa tcgcacccgg gcgcgtccgt
     1441 cgtggccgcg gcgctggcgc tgggcgagag ccgcggcatc gacggtgcgc gcttcatgcg
     1501 cgccgtcgcg ctcggctacg acgtcggccc gcgcgtcacg atggcgctcg gcggcgtcga
     1561 gttccgcaac gaaacacgcc gcagcacgca cgccttcgcc ggcacgttcg gcgcgtccgc
     1621 ggcagccgcg tgcgcggcgg gtctcgacgc acgccagatg cgctggctgc tcgattacgc
     1681 gtcgcagcaa gcggccggct acgcggtctg gggccgcgac acggagcacg tcgaaaaggc
     1741 cttcgtcttc ggcggcatgc cggcgcgcgc cggcgtgacg gccgccgagc tcgtcgctgc
     1801 gggctggagc ggcgtcgacg acgtgttctc cggcgaggac aacttcttcg cggtcaacgc
     1861 accgaatggg gatccgggcg tgctcgtcgc ggagctcggc gcgcgctacg aggttggcaa
     1921 caccgacatc aagaagtgga gcgtcggcac gccgatccag gcgccgctcg acgcgctcga
     1981 agcgttgcgc aagcaacggc cgttccgcgc cgccgacgtg accgaggtcg tcgtgcgctt
     2041 ggcgccgacg gtcggcgccg tcgtcgacaa ccgcgacatc cccgacatct gcttgcagca
     2101 catggtcgcc gtcatgctgc tcgacggcac ggcgtcgttc gccgcggcgc acgacgccgc
     2161 gcgcatgcaa gacgcggccg tgctacgcga gcgcgcgaaa gttcgctacg tgccggatgc
     2221 gcagctcgcg gcgctgttgc ccgcgcgcgt ggccgtcgtc gaggtcgcgt tgcgcgacgg
     2281 cacgcgcatt aatgagcgag tcgaggccgt gcgcggcacc gttcgtaatc cgatgagcag
     2341 agaagaggtc gtggcgaaag cgcgcgatct cgtcgagccg gtgttcggcg tcgcgaccgc
     2401 gggccgcc
//
LOCUS       DATLEE010000681         2703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3549758, whole genome shotgun sequence.
ACCESSION   DATLEE010000681 DATLEE010000000
VERSION     DATLEE010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3549758"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VM692_14875"
     CDS             <1..333
                     /locus_tag="VM692_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53507.1"
                     /translation="QTQTMRAIAVAKSIDDISSSMAETLFGDAELDLVTAALASAAEW
                     PDDDAPPSTTADKPEAVKVAPKPAAAKAPPPVEDPFDLFGLGDDAPLELIDDSTLPPI
                     APKSAARR"
     gene            complement(340..1257)
                     /gene="prpB"
                     /locus_tag="VM692_14880"
     CDS             complement(340..1257)
                     /gene="prpB"
                     /locus_tag="VM692_14880"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017012981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HUQ53508.1"
                     /translation="MSQGKKLRKLVATQAPLQIPGAINAYCALLARDAGFKALYLSGA
                     GVANASFGLPDLGVTTLGDVLEDARRVTAATDLPLLVDVDTGFGGAFNIARTVREMER
                     VGVAAIHIEDQEQAKRCGHRPNKSTVTVDEMCDRLKAAVDARRDDGFIVMARTDALAR
                     EGRSAALERAAAYVAAGADMIFAEALYTLDDYAAFTGAVDVPVLANLTEFGLTPYFTV
                     AELKRAGVKLVLYPLSAFRAMSRAAERVYAAIRNEGTQRAVLETMQTRDELYTVLDYH
                     SYERKLDELFASETKRTTAKRKVARRPKA"
     gene            complement(1254..2693)
                     /locus_tag="VM692_14885"
     CDS             complement(1254..2693)
                     /locus_tag="VM692_14885"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006037088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HUQ53509.1"
                     /translation="MSSHDGRSAERPAPDLLLTEIADYVLRDRADSAEARRIARYCLM
                     DTLGCGILALGFPACTKLLGPIVPGATLTGGARVPGTRYELDPVAAAFNIGTLIRWLD
                     FNDTWLAAEWGHPSDNLGAILGVADYENRRGVRLTMADVLTAMIQAHEIQGVLALNHS
                     FNRVGLDHVLLVRIASTAVATRMLGGSREEIVNAVSNAWLDGGALRTYRHAPNTGSRK
                     SWAAGDATSRGVRHALLARAGEMGYPSALTAKGWGFQDVLFKGRPIALERPLSSYVME
                     NVLFKISFPAEFHAQTAVEAALKLHSIVAPRVPEIERIVIETQEPGVRIIDKTGPLAN
                     PADRDHCLQYMVAVPLLIGRLTAEDYEDKVAADPRIDALRAKMVVTENLGFSRDYLDP
                     DKRAIGNAVQVFFANGTHTERVAVDYPIGHRRRRAEGIPQLEAKFERNLATLFGARQG
                     TAIAAACADQARFEALPVADFMALWVPQS"
BASE COUNT          433 a          930 c          900 g          440 t
ORIGIN      
        1 cagacgcaaa cgatgcgcgc gattgccgtc gcgaaatcga tcgacgacat ctcgagctcg
       61 atggccgaga cgttgttcgg cgacgcggag ctcgatctcg tgacggcagc cttggcctcg
      121 gccgcggagt ggcccgacga cgacgcgcca ccgtcaacca ctgccgacaa gcctgaggcg
      181 gtgaaggttg cgcccaaacc ggcggccgcg aaggcgcctc cgcccgtcga ggacccgttc
      241 gacctctttg gtctcggcga cgatgcgccg ctcgagctca tcgacgattc gacgttgccg
      301 cccatcgccc cgaagagcgc agcgcgccgc taacggagcc taagccttcg gccgccgcgc
      361 gaccttgcgt ttcgcggtcg tccgcttcgt ctcgctcgcg aacagctcgt cgagcttgcg
      421 ctcgtaagag tgatagtcga gcacggtgta gagctcgtcg cgcgtctgca tcgtctcgag
      481 caccgcgcgt tgcgttccct cattgcgtat cgccgcgtag acgcgctcgg cggcgcggct
      541 catcgcgcga aaggccgaca acggatacag cacgagcttg acgccggcgc gtttcagctc
      601 ggcgaccgtg aaatatggcg tcagcccgaa ctccgtgaga ttcgcgagca cgggcacgtc
      661 gacggccccc gtgaaggccg cgtagtcgtc gagcgtgtac agcgcctcgg caaaaatcat
      721 gtcggcgccc gcggcgacat acgccgccgc gcgctcgagc gcggccgagc ggccctcgcg
      781 cgcgagcgca tccgtgcgtg ccatgacgat gaagccgtcg tcgcgccgcg cgtcgacggc
      841 cgccttcagc cggtcgcaca tctcgtcgac ggtcaccgtc gacttgttcg gccgatggcc
      901 gcaacgcttc gcttgctcct gatcttcgat gtggatcgcc gcgactccga cacgctccat
      961 ctcgcgcacc gtgcgcgcga tgttgaacgc gccgccgaac ccggtgtcga cgtcgacgag
     1021 cagcggcaag tccgtcgccg ccgtgacgcg gcgcgcgtcc tcgagcacgt cgcccagcgt
     1081 cgtgacgccg agatcgggca acccgaacga cgcattcgcg actccggcgc cggacagata
     1141 cagcgccttg aacccggcgt cgcgtgccag cagcgcgcaa tacgcattga tcgcgccggg
     1201 gatctgcagc ggcgcttgcg tcgcgacgag cttacgcagc tttttgccct ggctcacgat
     1261 tgcggcaccc agagcgccat gaagtcagcg accggcagcg cttcgaagcg cgcttggtcc
     1321 gcgcacgcgg ctgcaatcgc ggtgccctgc ctcgcgccga acaaggtcgc gagattgcgt
     1381 tcgaacttcg cctcgagctg cggaatacct tcggcgcgcc ggcgccgatg gccgatcgga
     1441 tagtcgacgg cgacgcgctc ggtgtgcgtg ccattcgcga aaaacacttg cacggcgttg
     1501 ccgatcgcgc gcttgtcggg gtccagatag tcgcggctga agcccaagtt ctcggtcacg
     1561 accatcttcg cgcgcagcgc atcgatacgc gggtcggcgg cgaccttgtc ttcgtagtcc
     1621 tcggctgtca aacgcccgat cagcagcggc acggcgacca tgtactgcaa gcaatggtcg
     1681 cgatcggcag ggttcgcaag cgggccggtc ttgtcgatga tgcggacgcc gggctcctgc
     1741 gtctcgatga cgatgcgctc gatctccggc acgcgcggcg cgacgatgga gtgcagcttc
     1801 agcgccgcct cgaccgccgt ttgcgcgtgg aactcggccg ggaacgagat cttgaacagc
     1861 acgttctcca tgacgtacga gctcaagggc cgctcgagag caatcggccg ccctttgaac
     1921 agcacgtcct ggaagcccca gcctttggcc gtcaacgccg acgggtaacc catctcgccc
     1981 gcgcgggcga gcagcgcgtg ccgcactccg cggctcgtcg catcgccggc ggcccagctc
     2041 ttgcgcgagc ccgtgttcgg cgcgtggcga tacgtccgca gcgcgccgcc gtcgagccag
     2101 gcgttcgaga cggcgttgac gatctcctcg cgactgccgc cgagcatacg cgtcgcgacg
     2161 gccgtgctcg cgatgcgcac gagcaggacg tgatcgagac ccacgcgatt gaagctgtgg
     2221 ttcaacgcga gcacgccttg aatctcgtgc gcttggatca ttgccgtgag gacgtcggcc
     2281 atcgtgagcc gcacgccgcg gcggttctcg taatcggcga cgccgagaat tgcgccgagg
     2341 ttatccgatg ggtggcccca ctctgcggcg agccacgtgt cgttgaagtc gagccagcga
     2401 atcagcgtgc cgatgttgaa tgcggcggcg acggggtcga gctcgtagcg cgtgccgggc
     2461 acgcgcgcgc cgccggtcaa cgtcgcgccc ggcacgatcg gccccagcaa cttcgtgcat
     2521 gccgggaacc cgagcgccaa gatgccgcag ccgagcgtgt ccatgagaca gtagcgcgca
     2581 atgcgacggg cttcggcgct gtcggcgcga tcgcgcaaca cgtagtcggc gatttccgtg
     2641 agtagcagat ccggtgcggg gcgctcggcg ctgcggccgt cgtgggagct catcagatgc
     2701 aac
//
LOCUS       DATLEE010000682         4282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10150517, whole genome shotgun sequence.
ACCESSION   DATLEE010000682 DATLEE010000000
VERSION     DATLEE010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10150517"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VM692_14890"
     CDS             <1..320
                     /locus_tag="VM692_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53510.1"
                     /translation="LGDARGHWEGNTLVVETTNFTDKVPYRGSSEHLKLVERFTPLGP
                     DTLEWSVTFEDPHTWERPWTFAMNLVHDESQPLFEYACHEGNYGLANILSTARAEEAA
                     AKK"
     gene            361..738
                     /locus_tag="VM692_14895"
     CDS             361..738
                     /locus_tag="VM692_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53511.1"
                     /translation="MHQLRSRAATPAILSGLATALLALGATASAQAPSAGQMKSLSHN
                     IHAVNDLDTTLAFYRDVFGLDGRALDFANPAVPLLTNAPGVTLRMSMLTLPGGTRYEL
                     THFKGLSGSPSKPTTGIPVRRAS"
     gene            738..1400
                     /locus_tag="VM692_14900"
     CDS             738..1400
                     /locus_tag="VM692_14900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ53512.1"
                     /translation="MYVRDLDSLVAAAKKVKAPIVTTGGAPVHVATAAGQARSIVIRD
                     PDGYFLQLVEEAPAAGAPEGPIHRTSLAFTMEDAAATQKFYTGMMAVPLTGPTEFKRD
                     AELAKLFGAPETVEFRKLSGTLPPGTAVEFTEFRGVPRTKFHLRVRDPGAPAMAINVD
                     NLQGMVAQMKAAGVNLISTNGELVDFGNGVRNIFVEDPNGMNLELIYRPATPPPAAQP
                     SR"
     gene            complement(1425..2606)
                     /locus_tag="VM692_14905"
     CDS             complement(1425..2606)
                     /locus_tag="VM692_14905"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ53513.1"
                     /translation="MNRLSKALAACCVSLAGIAPAVAQDLTITNAHIVVGNGTVIERG
                     SIVVRGGRIVSVAAGAPASTSGQVIDAKGMSAMAGFIDAHRHINTGPEEKAQMQRQLE
                     AGYTTILSGGGPGDGNITLRQHIESGQINGPRVIPSERLRLAGLTPEAGREAVRKIKA
                     MGIDYTGEISLTPVPGPSAEELATLRAVLDEGKKVGVKVQVHAVSSPAMNAAVEMGIP
                     LLVHLPNKDWVSKADAQKLAAAGTKILMTIGFGAPTIDVFAKDNTPRFRDGKPWPDGI
                     VDGVGGGVEAGYAMVNARTIFDAGGIIGYCTDTTYDALAGLEHELKSMNVVFSPTDMI
                     KIMGPNTASYLGMEKDLGTLEAGKLADILLLDGDPLEGYWNWLKTKVTIKEGKVVVDA
                     R"
     gene            2835..3662
                     /locus_tag="VM692_14910"
     CDS             2835..3662
                     /locus_tag="VM692_14910"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HUQ53514.1"
                     /translation="MHRARRHQALRTVWLSDIHLGSPECRVNLLLDFLRHTRCEQLYL
                     VGDIIDLEHLRQEFYWPASHTEALRLLLKKSQEGTRIVYLPGNHDHDLHKMAGVKLGN
                     IEIASQAIHTTRDGRRLLVTHGDQFDAVVRRPSLGVWLGGFACRRLLTLNRLVHWVHD
                     LLDLPYWSLAQHLKDRFPGAQRYIERYQNATLAAARAAGVDGVVCGHIHRADLRTVDG
                     LTYCNDGDWVESCTALVEDLRGELSLLKWRPTAAAIAASASIAIWADAITESEQEAA"
     gene            complement(4100..>4282)
                     /locus_tag="VM692_14915"
     CDS             complement(4100..>4282)
                     /locus_tag="VM692_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53515.1"
                     /translation="VAIGLAASAMFNRPFFTGSWEWSETIPFVMFVMLPFGATAWLLI
                     KSRPFLLAEPRTRAAS"
BASE COUNT          759 a         1453 c         1351 g          719 t
ORIGIN      
        1 acttgggcga cgcgcgcggt cattgggaag gcaacacgct cgtcgtcgag acgacgaact
       61 tcacggacaa ggtgccgtat cgcggctcga gcgagcactt gaagctcgtc gagcgcttta
      121 cgccgctcgg ccccgacacg ctcgagtggt cggtgacgtt cgaggacccg cacacgtggg
      181 agcggccgtg gacgttcgcg atgaacctgg tccacgacga gtcgcagccg ttgttcgagt
      241 acgcctgcca cgaaggcaac tacggccttg ccaacattct ttcgacggcg cgcgctgagg
      301 aagcggcggc caaaaaataa cgcggcgtcg agagccgcaa ccacgcaacg ggggacgtcc
      361 atgcatcagc ttcgatcacg cgccgcgacg ccggcgatcc tgtccggcct cgccactgcc
      421 ttgctcgcgc tcggcgcgac cgcgtccgcg caagctccgt ccgccgggca aatgaaatcg
      481 ctgtcgcaca acattcacgc cgtcaacgac ctcgacacga cgctcgcgtt ctaccgtgac
      541 gtcttcggcc tcgacggccg ggcgctcgat ttcgcgaatc ccgccgtgcc gctgctgacg
      601 aatgcgccgg gcgtcacgct gcgcatgtcg atgctgacgt tgccgggcgg cacgcgctac
      661 gagctcacgc atttcaaagg gctgagcggc agcccaagca agccaactac tgggatcccg
      721 gtgcggcgag cgtcgtaatg tacgtgcgcg atctcgactc gctcgtcgcg gccgccaaga
      781 aggtcaaggc gccgatcgtc acgacgggag gcgcgcccgt ccacgtcgcg acggccgccg
      841 gccaagcacg ctcgatcgtg atccgcgatc ccgacggcta cttcttgcag ctcgtcgagg
      901 aagcgccggc ggctggtgct ccggagggcc cgatacaccg cacgtcgctg gcgttcacga
      961 tggaggacgc cgccgccacg cagaagttct acaccggcat gatggccgtg cccctcacgg
     1021 gcccaaccga gttcaagcgc gacgcggaat tggcgaagct gttcggagcg cccgaaaccg
     1081 tcgagttccg caagttgagc ggcacgttgc ccccgggcac ggcggtcgag ttcacggagt
     1141 tccgcggcgt gccgcgcacg aagttccact tgcgcgtgcg cgatcccggc gcgccggcca
     1201 tggcgatcaa cgtcgacaac ctgcaaggca tggtcgcgca gatgaaggcc gccggcgtca
     1261 acttgatctc caccaatgga gagctcgtcg acttcggcaa cggcgtccgc aacatcttcg
     1321 tcgaggaccc gaacggcatg aacctcgagc tgatctaccg gccggcgacg ccgccaccgg
     1381 ctgcgcaacc gagccgttag cttcgagcgt cgtcgcgacg cgcgtcagcg cgcgtcgacg
     1441 acgaccttgc cttccttgat ggtgaccttg gtcttgagcc agttccagta gccctcgagc
     1501 gggtcaccgt cgagcagcag gatatcggcg agcttgccgg cttcgagcgt gcccaagtcc
     1561 ttttccatgc cgagatacga ggccgtgttc ggccccatga tcttgatcat gtccgtcggc
     1621 gagaacacga cgttcatcga cttcagctcg tgctcgagac cggcgagcgc gtcgtaggtc
     1681 gtgtccgtgc agtagccgat gatgccgccc gcgtcgaaga tcgtgcgtgc gttgaccatc
     1741 gcgtagccgg cctcgacgcc gccgccgacg ccgtcgacga tgccgtcggg ccatggcttg
     1801 ccgtcgcgga agcgcggcgt gttgtccttc gcgaacacgt cgatcgtcgg cgccccgaag
     1861 ccgatcgtca tgaggatctt cgtgccggcc gccgcgagct tctgcgcgtc ggccttgctg
     1921 acccagtcct tgttcggcaa gtggacgagc agcggaatgc ccatctctac ggcggcattc
     1981 atcgccggcg agctgacggc gtggacctgg accttgacgc cgactttctt gccttcgtcg
     2041 agcaccgcgc gcagcgtcgc gagctcttcc gcactgggac ccggaacggg cgtcagcgag
     2101 atctcgcccg tgtagtcgat acccatcgct ttgatcttgc gaaccgcctc gcgccccgcc
     2161 tccggcgtca gcccggcgag ccgcaaacgc tccgacggaa tgacccgcgg accgttgatc
     2221 tgccctgact cgatgtgctg ccgcagcgtg atgttgccgt ccccggggcc gccgcccgac
     2281 agaatcgtcg tatagcctgc ctcgagctgg cgctgcatct gcgccttctc ctcagggccc
     2341 gtgttgatgt gccggtgcgc gtcgatgaag cccgccatcg cgctcattcc tttggcgtcg
     2401 atgacctggc cgctcgtgct cgccggcgcg cccgccgcca cggagacgat gcgcccgccg
     2461 cgaacgacga tcgagccgcg ctcgatgacc gtgccgttgc cgacgacgat gtgggcgttc
     2521 gtgatcgtga ggtcctgcgc cacggccggc gcgataccgg ccaagctcac acagcaggcc
     2581 gctaaggctt tggaaagtct gttcattttg gtttcagctc cgttgggtag gctcatctta
     2641 atacggcgcc gtcgccgtcc gacgacagtc gcaaccgagg acgggaccga ttacccttcg
     2701 cgccgaagct cgagccaaag cgatcgagct cacagaaccc aggaagggtg gcagacgcgg
     2761 tgaagttttt gcgatccgcg tcgacgtaaa tcggccgcaa gaactagagt ctgcggccct
     2821 aatggaggga cccggtgcat agagctcggc gacatcaggc gctgcgtacc gtctggctgt
     2881 cggatattca tctcggctcg cccgagtgcc gcgtgaacct gctgctcgat ttcctgcgcc
     2941 acacgcgctg cgagcagctc tacctcgtcg gcgacatcat cgacctcgag cacttgcgcc
     3001 aggagttcta ctggcccgcg agccacacgg aggccctgcg cctcttgttg aagaagagcc
     3061 aagaaggcac gcgcatcgtc tatctgcccg gcaaccacga tcacgacctg cacaagatgg
     3121 ccggcgtgaa gctgggcaac attgagatcg cgtcgcaggc gattcacacc acgcgcgacg
     3181 gccgccgctt gctcgtgacg cacggcgatc agttcgatgc cgtcgtgcgc cgtccgtcgc
     3241 tcggcgtctg gctcgggggc tttgcgtgcc ggcgcttgct gacgctgaat cgcctcgtgc
     3301 attgggtgca cgatttgctc gaccttccct actggtcgct cgcgcagcac ttgaaggatc
     3361 gtttcccggg agcgcagcgc tacatcgagc gttatcaaaa tgcgacgctc gcggccgcgc
     3421 gcgcggctgg cgtcgatggc gtcgtctgcg gccacattca tcgcgccgat ctgcgcacgg
     3481 tcgacggctt gacctactgc aacgacggcg attgggtcga gagctgcacg gcgctcgtcg
     3541 aggacctgcg cggcgagttg tcgctgctca agtggcggcc aacggcggcc gcgatcgccg
     3601 cgtctgcgtc gatcgccatc tgggctgacg cgattacgga atccgagcaa gaagctgcct
     3661 gaccttgcgt caactcccgc caatatcgtc acgagcgcga acgtcgcgag ccgatgaaat
     3721 aggcgccgat gtgcgccagt gccacgacca tcggcgacga accagaacca atccgaccac
     3781 ggctgcgtcg cagcgaaact gagatcctcg gccaagacca tcccatagcc agtggctgcg
     3841 atgccgagca ctaaaccgag caccacacga ggcgtcctaa cgccacgtgc tgcactgatc
     3901 agcgcagcgt aacccctgac accggcggct ccggccagca tgaccgccga tgagcggagg
     3961 aaagcctccg cgtcgatcga gtcgaacttc gtcagtagcg caaacggcgc catgatcagc
     4021 agcagcccgg cccaaaacac caggtacgtg gcgggaacga cgcctagcac gaagctcgcg
     4081 gcggggatca gccaccggct catgaggcag cacgcgtgcg cggttcagcg agcaagaatg
     4141 gccgcgattt gatcagcagc cacgcggtgg cgccaaacgg cagcatcacg aacatcacga
     4201 agggtatggt ctccgaccac tcccaagacc cggtaaaaaa cggccgattg aacattgccg
     4261 acgccgccag gccgatggcg ac
//
LOCUS       DATLEE010000683         3962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8757995, whole genome shotgun sequence.
ACCESSION   DATLEE010000683 DATLEE010000000
VERSION     DATLEE010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8757995"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VM692_14920"
     CDS             <1..385
                     /locus_tag="VM692_14920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HUQ53516.1"
                     /translation="VLADATRVVAGVNPLCGDKMTLYVKLADGKIEDIAFQGTGCAIS
                     VASSSLMTERVKGAPVTAALELFDKIHGLLTAPHDHDPPADLEKLAALAGVREYPSRV
                     KCASLGWHALKSAVTDAPSSNVTTE"
     gene            400..951
                     /gene="sufT"
                     /locus_tag="VM692_14925"
     CDS             400..951
                     /gene="sufT"
                     /locus_tag="VM692_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HUQ53517.1"
                     /translation="MYHSTNEPVTFSRDCPAVLIPAGEHVTLPAGTTGFMTQALGGSF
                     TIYIEGNLFRVAGRDGDAIGKPALSGPDLPDDATEADIESAVWQQLRTCYDPEIPINI
                     VELGLVYECKIERAASGGRVAKVRMTLTAPGCGMGEILTQDVREKIEMIPTVERADVE
                     LVFDPPWNQSMMSEEARLEAGLL"
     gene            976..1494
                     /locus_tag="VM692_14930"
     CDS             976..1494
                     /locus_tag="VM692_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HUQ53518.1"
                     /translation="MKRLLLLRHAKAVPAEEPLADIARPLADRGERDARRVGERLRQH
                     QEPPGRILTSPAARTLQTAQIVAAAFAERADALVIERRLYLAEPAALLDVIANQEPHL
                     DSLLVVGHNPGLSELVHDLLPQFEVDDLPTCAIVALDYPAGAEWLRIETAAPRLAYYD
                     FPKNSREPVTAR"
     gene            complement(1481..1909)
                     /locus_tag="VM692_14935"
     CDS             complement(1481..1909)
                     /locus_tag="VM692_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HUQ53519.1"
                     /translation="MADERDDGDASDRLRIDRWLWCARFYKSRSAAAEAVRGGHVRLN
                     GQRVKPAHAVKLGDALNVELPRSPEREITIAAIPARRGPAPEAAATYVESVESVERRR
                     RAAEERAARTAYLPPTQGKPDKRTRRLLLRARQRGPLSEP"
     gene            complement(1913..3781)
                     /gene="sppA"
                     /locus_tag="VM692_14940"
     CDS             complement(1913..3781)
                     /gene="sppA"
                     /locus_tag="VM692_14940"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HUQ53520.1"
                     /translation="MDSDNRSLPLRIVAGIWRGLDRLRRFLHLIFMLGIFLLLLIAAI
                     GERVYIPGTAALVIAPQGALVDQLSGDALGRAIAKAQGAPLQETLVRDVVEALRAARD
                     DERIKAVVLDLDGMTGAGLSKLQEIAAEIVQFKQSGKPVIAVGDGFTRDQYYLAAQAD
                     NVYMHPMGLVLVDGYSRFLPYYKSLLDKAYVDYNVWTVGEYKSFVEPITRDDMSPQDE
                     EASRLFLNGLWAAYQADVTAARELAAPALQQYADDIVTLLEESGGDSAKLAVDYGLVD
                     ELLTRDAMRERIREAIGEDAAREQTSDDYTEVSLEAYVAALDTETPPEVLPSKIAVIV
                     ASGTILDGAQPPGAIGGDSTAQLVRQARADDAVKALVLRVDSGGGSAFASDVILRELE
                     RFQETGRPVVVSMGSVAASGGYWISMGADEIWASPTTLTGSIGVGATIPTIPRTLDWL
                     GVHVDGIGTTKLAGALEITRPLGDEVKGLIGLSIRKTYDDFVRKVAEHRERTVEEIDA
                     AAHGRVWTGADALDRGLVDKLGTLSDAIDSAAELAGLERGTYSLDYVEQQLGVAERLV
                     LSLTAKAAVGVDHLVKLPRLPTNVTQALESTLEPLAFLDRLNDPRGIYVYCFCDTK"
     gene            complement(3803..>3962)
                     /locus_tag="VM692_14945"
     CDS             complement(3803..>3962)
                     /locus_tag="VM692_14945"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HUQ53521.1"
                     /translation="PPVGLNLFIASHRFDKDITEIYWATQPFLLVSLLAVIIIAFWPD
                     LSLMLLRR"
BASE COUNT          672 a         1334 c         1298 g          658 t
ORIGIN      
        1 cgtgctcgcc gatgcgacac gcgtggtggc cggcgtcaat ccgctctgcg gcgacaagat
       61 gactctgtac gtgaagctcg ccgacggcaa gattgaagac atcgcgtttc agggcacggg
      121 ctgcgctatt tccgtcgctt cgtcgtcgtt gatgacggag cgtgtgaaag gcgcgccggt
      181 aacggcagcc ctcgagctgt tcgacaagat tcacgggctg ttgaccgcgc cgcacgatca
      241 cgacccgcca gccgatctag aaaagctcgc ggcactcgcc ggcgtgcgcg agtacccgtc
      301 gcgcgtcaaa tgcgcgagcc tcggttggca tgcgctgaag tcggcagtca ccgacgctcc
      361 ctcgagcaac gtgacgaccg agtagcgggc tcgaacgaca tgtaccactc gaccaacgag
      421 cccgtaacct tttcgcgcga ctgccccgcc gtgctgattc cggccggcga gcacgtcacg
      481 ttgccggcgg gcacgacggg gttcatgacg caagcgctcg gcggcagctt tacgatctat
      541 atcgaaggca atctgttccg cgtcgccggc cgcgacggcg acgcgatcgg caagccggcg
      601 ctctcgggcc ccgacttgcc cgacgacgcg acggaggccg acatcgagtc ggccgtctgg
      661 cagcagctgc gaacttgcta cgaccccgag ataccgatca acatcgtcga gctcggcctc
      721 gtctatgaat gcaagatcga acgcgcggct tccggcgggc gcgtcgcgaa agtcaggatg
      781 acgttgacgg cgcccggctg cggcatgggc gagatcctga ctcaagacgt ccgcgaaaag
      841 atcgagatga tcccgaccgt cgagcgcgcc gacgtcgagc tcgtgttcga cccgccctgg
      901 aatcaatcga tgatgtcgga agaggcgcgg ctggaagcgg gattgttgtg aagcgtcgcg
      961 cctgaagcct tactgatgaa gcgtttgctg ctgttgcgcc atgcgaaggc cgtgccggcg
     1021 gaggagcccc tggccgatat cgcgcggccg ctcgccgacc gcggcgaacg cgacgctcga
     1081 cgcgtcggtg agcgcctacg ccaacatcaa gagccgccgg ggcggatact gacgagcccc
     1141 gccgcgcgga cactgcagac ggcgcaaatc gtcgcggcgg cgttcgcgga gcgcgctgac
     1201 gcgctcgtga tcgagcgacg cctgtacctg gccgagcctg cggcactgtt ggacgtcatc
     1261 gcgaatcaag agccgcacct cgactcgctg ctcgtcgtcg gccataaccc gggtttgagt
     1321 gagctcgtcc acgacctgct gccgcaattc gaagtcgacg accttccgac gtgcgccatc
     1381 gtcgcgctcg actatccggc cggcgcggaa tggctgcgga tcgagacggc tgcgccccgc
     1441 ctcgcctatt acgacttccc gaagaactcg cgcgagccgg tcacggctcg ctgagcggcc
     1501 cgcgttgccg tgctcgcagc agcagccggc gcgtacgttt gtcgggcttg ccttgcgtcg
     1561 gagggagata ggccgtgcgc gcggcgcgct cctcggccgc tcgccgccgg cgctcgaccg
     1621 actccacgct ctcgacgtac gtcgcagcag cctcgggcgc gggcccgcgc cgagccggga
     1681 tcgccgctat cgtgatctcg cgctcgggtg agcgcggcag ctcgacattc agtgcatcgc
     1741 cgagcttgac ggcatgcgca ggcttcacgc gctggccatt taagcgcacg tggccgccgc
     1801 gaacggcttc tgccgcggcg ctgcggctct tgtagaagcg cgcgcaccag agccagcggt
     1861 cgatgcgaag ccggtcggac gcgtccccgt catcgcgctc gtcggccatg cgtcatttcg
     1921 tgtcgcaaaa acagtagacg tagataccac gcggatcgtt gaggcgatcg aggaatgcca
     1981 gcggctccag cgtcgattcg agcgcctgcg tgacgttcgt cggcaaccgc ggcagcttca
     2041 cgagatggtc gaccccgacc gcggccttcg ccgtcagcga caataccaag cgctccgcga
     2101 cgccgagctg ttgctccacg tagtcgagcg aatacgttcc gcgctcgagg cccgcgagct
     2161 cggcggcgga atcgatcgcg tcgctcaacg tgccgagctt gtcgaccaac ccgcggtcga
     2221 gcgcgtcggc gccggtccac acacggccgt gcgcggccgc gtcgatctcc tcgacggtgc
     2281 gctcgcggtg ctccgcgacc ttgcgaacga aatcgtcgta ggttttgcgg atcgatagcc
     2341 cgatgaggcc cttgacctcg tcgccgagcg gccgcgtgat ctcgagcgcg ccggcgagct
     2401 tcgtcgtgcc gatgccgtcg acgtgcacac cgagccagtc gagcgtgcgc gggatcgtgg
     2461 gaatcgtcgc gccgacgccg atcgaccccg tcaaagtcgt cgggctcgcc catatttcat
     2521 cggcgcccat cgagatccaa tagccgccgg acgcagcgac gctgcccatc gagacgacga
     2581 cgggccggcc cgtctcttgg aaccgctcga gctcgcgcag gatgacatcg gacgcgaacg
     2641 cgctgccgcc gcccgagtcg acacgcaaca cgagcgcctt gacggcgtcg tccgcgcgcg
     2701 cttgccgaac cagctgagcc gtcgagtcgc cgccgattgc gcccggaggc tgcgcgccgt
     2761 cgagaatcgt gcccgacgcc acgatgacgg cgatcttgct cggtaacacc tcgggcggcg
     2821 tctccgtgtc cagtgccgcc acgtaggctt cgagcgacac ctccgtgtag tcgtcgctcg
     2881 tctgctctcg cgccgcgtcc tcgccgatgg cctcacggat gcgctcgcgc atggcatcgc
     2941 gcgtcagcag ctcgtcgacg agcccgtagt cgacggcgag ctttgcggaa tcgccgccgg
     3001 actcctcgag cagcgtcacg atgtcgtcgg cgtattgctg cagcgccggc gccgccagct
     3061 cgcgcgcggc cgtcacgtcg gcctggtaag ccgcccataa cccgttgagg aacagcctgc
     3121 tcgcctcctc gtcctgcggc gacatatcgt cgcgcgtaat cggctccacg aaggacttgt
     3181 actcgccgac cgtccaaacg ttgtagtcga cgtacgcttt gtcgagcagt gacttgtagt
     3241 acggcaggaa gcggctgtag ccatcgacga gcacgaggcc cattgggtgc atgtagacgt
     3301 tgtcggcttg cgccgcgagg tagtactgat cgcgcgtaaa gccgtcgccg acggcaatga
     3361 cgggtttgcc gctttgcttg aactgcacga tttcggcagc gatctcctgc agcttgctga
     3421 gccctgcacc ggtcatgccg tcgagatcga gcacgacggc tttgatgcgc tcgtcgtcgc
     3481 gcgccgcgcg cagcgcctcg acgacgtcgc gcacgagcgt ctcttgcagc ggcgcgccct
     3541 gggccttcgc gatcgcgcgc ccgagcgcat cgccgctcag ctgatcgacc agcgcgcctt
     3601 gcggtgcaat gacgagcgct gcggtgcccg gaatgtagac gcgctcgccg atcgcggcga
     3661 tcagcaacag caagaagatg ccgagcatga aaatcaaatg cagaaatcgg cgcaggcgat
     3721 cgagcccgcg ccaaatgccc gcgacgattc gcaacggcaa cgagcgattg tcactgtcca
     3781 tggtggttac ctgagaagcc gcttaccggc ggagcagcat taatgataga tcgggccaga
     3841 aggcgatgat gatgacggct agcaacgaga cgagcaggaa cggttgcgtc gcccaataga
     3901 tctccgtgat gtccttgtcg aagcgatggc ttgcgatgaa caaattgaga ccgaccggcg
     3961 gc
//
LOCUS       DATLEE010000684         5853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6424312, whole genome shotgun sequence.
ACCESSION   DATLEE010000684 DATLEE010000000
VERSION     DATLEE010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6424312"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VM692_14950"
     CDS             complement(<1..545)
                     /locus_tag="VM692_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53522.1"
                     /translation="MSLSGNDVLALIERTLTDTRGEVGNIDMRLMRATAELERLRQAE
                     LGCLSVLAKMRLREIEGAEVSASLDATGSSVRELLAQREVAQAANEADIARADDRRKE
                     LESERAAQHAVVAAAEQALDEARAAAQKALAADEHYRAAMAAAEASDRVADLAEDKAK
                     AAHKDREEKGKPYEADPLFSY"
     gene            complement(550..1464)
                     /locus_tag="VM692_14955"
     CDS             complement(550..1464)
                     /locus_tag="VM692_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6384 family protein"
                     /protein_id="HUQ53523.1"
                     /translation="MNDVVVKEKAPLDELMLAMDVVDTLRHKELVLARELSAEDRDAQ
                     LLEQLREIYTSQGIVVTDEILQQGVDALREERFVYPQPEPSFSRTLAHAYVTRGRWGK
                     AAGVGVAAVAVAAVAMQLFVWGPQQRQTAEAPAELTAAFATLDANTDDPAALDPARAQ
                     LATGQAAVAQGDFATARAATAEIETLNARVQAQYELRVVSRQGEQSGVWREPQVNRGA
                     RNYYLIVEAVAPNGEALRLPITSEEDGRTRAVRAWGLRVDEATYNRVGADKQDDGIIQ
                     NNVVGRKRRGVLEREYTVPTTGAAITEW"
     gene            complement(1496..2668)
                     /locus_tag="VM692_14960"
     CDS             complement(1496..2668)
                     /locus_tag="VM692_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HUQ53524.1"
                     /translation="MATPIQYLDKAMTRLRDLGLVPEKPEEAPIVALLNRVSDLDNDK
                     VVAIARTLSQASLFNQVVREQVASMSLGERYAKITEGFDSIRDDAKTMVKQVEDGRID
                     TFERMANVWQKATRGDIAGRFDKVKKVYLDVVKDSKDQIEREQVILQAYLDFRGALKE
                     SEVLALQVLKVAEGELAAAQAQLKVAVEAVEKAGSDDLVARAKLELKRDEELRNVQGA
                     EDRYQICKDLSDNLTIGYNTSEVIMARLVQTTNAKDRVYKQAVSFFGTNETVLTALSA
                     SFTGLWGLHESTQTLDAMKEGVSKSLEDLADIGGKVQEAALKAGYGPTIRADAVKKLV
                     DSVVNYQLRSREIIAEMRDQSTKNAAEIRDAVEQGKQRLAKLVESGQALELVAQTR"
     gene            2941..3732
                     /locus_tag="VM692_14965"
     CDS             2941..3732
                     /locus_tag="VM692_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HUQ53525.1"
                     /translation="MPTAHEIDYEIRGNDMQYVEVELDPGESAVAEAGSLMYKDVAVE
                     MTTVFGDGGGQTSGLMGMLVGAGKRLLTGESLFMTVFTHNGSGKARVAFAAPYPGTIL
                     AMDLKKLGGKLICQKDAFLAAAKGVSIGIAFQKRIMTGLFGGEGFIMQKLEGDGMVFV
                     HAGGTLVERELRAGEELHVDTGCIAALAPTVDYDIVTVGGVKSMIFGGEGVFFARLRG
                     PGHVWLQSLPFSRLAGRIIANLGPTGRRTDEGSVLGPLGDILRGS"
     gene            complement(3768..5612)
                     /locus_tag="VM692_14970"
     CDS             complement(3768..5612)
                     /locus_tag="VM692_14970"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ53526.1"
                     /translation="MNRIGFAVLGAAGVLIGAASAQPAASQNARAPVPRASAAPPKFT
                     AQQLTALPTANWITNGGNLYNQRYSPLARINKDNVATLRPAWRTHLNGSGTESKYSGQ
                     AQPIVYDGVIYVPTGANDVFALDADDGKILWTYEAHLDANITVICCGWLSRGVAIGDG
                     KVFSGQLDGKLVALDHATGKVVWSIQAETNQDGFSITGAPLYYDGLVITGFAGGDRAA
                     RGRVKAYDAKDGKLVWTFYTIPGPGEVGHDTWPANNDTWKYGGAAVWQTPAVDPELGL
                     VYFSTGNPGPDLIGSVRPGDNLFSVSMVAIEAKTGKYRWHFQQVHHDLWDYDSPNPVV
                     LFDATVDGRLRKGIVEIGKTGYVYILDRETGKPLVGIDEVAVPQEPRQATAATQPIPR
                     GDEVIPHAIDIEPEGFKLTNNGRIFTPFWDKPVVVKPLATGGVNWPPSSYDPTTNLFY
                     VCATDGAAAYSTKEGGVEWAIPTPGGRYFGGDYTRSQVPRRGVIAAVDVRTNKLAWQQ
                     QWGEMCYAGSTVTGGGLLFLGRNDGRLTAHDKSNGALLWEYQTDAGIHAAVSTFERNG
                     QQYIVALSAGSFFPGTTRGDSVYLFALDGAGVEGGARAEPSGGAELRH"
BASE COUNT          910 a         1967 c         1879 g         1097 t
ORIGIN      
        1 aaatacgaga acagcggatc ggcctcgtac ggcttgccct tctcctcgcg atccttgtgt
       61 gcggctttcg ccttgtcctc ggcgagatcc gcgacgcggt ccgacgcttc cgccgcggcc
      121 atggcggccc gataatgctc gtcggccgca agcgcctttt gcgcggcagc tcgcgcttcg
      181 tcgagtgcct gctcggccgc ggcgacgacg gcatgctgcg cggcgcgctc gctctcgagc
      241 tctttgcggc gatcgtctgc gcgcgcaatg tccgcctcgt ttgcggcctg cgccacctcg
      301 cgttgcgcga gcagctcacg cacgctcgag cccgtggcat cgagcgacgc ggaaacctcg
      361 gctccttcga tttctcgcaa ccgcatcttt gcgagcacgc tcaagcagcc gagctcggcc
      421 tggcgcaggc gctcgagctc ggccgtcgcg cgcatgaggc gcatgtcgat gttgccaacc
      481 tcgccgcgcg tatccgtcag cgtgcgctcg atcagcgcca gaacgtcgtt gccgctcagg
      541 gacatcgctt taccactccg taatcgcagc gcccgtcgtc ggcaccgtgt actcgcgctc
      601 gagaacgccg cgacgcttcc ggccgacgac gttgttttga atgatcccgt cgtcttgctt
      661 gtcggcaccg acgcggttgt aagtggcttc gtcgacgcgc aagccccagg cgcgcacggc
      721 acgcgtgcgg ccgtcctcct cgctcgtgat cggcagccgc agcgcctcgc cgttcggcgc
      781 gacggcttcg acgatcaagt aatagttgcg cgcacctcga ttgacttgcg gctcgcgcca
      841 gacgccgctc tgctcgcctt ggcgcgagac gacacgcagc tcgtattgcg cctgcacgcg
      901 cgcgttcagt gtctcgatct cggcggtggc ggcgcgcgcg gtagcgaagt cgccttgcgc
      961 aacggcggcc tggcccgtcg cgagctgcgc ccgcgcggga tcgagcgcgg ccgggtcgtc
     1021 ggtgttcgca tcgagcgtcg cgaacgcggc tgtcagctcg gcgggggctt cggcggtttg
     1081 ccgctgctgc ggcccccaaa cgaacagctg catcgctacg gccgcgacgg cgacggccgc
     1141 cacgccgacg ccggcggctt tgccccagcg gccgcgcgtc acgtaggcgt gcgctagcgt
     1201 gcgcgagaag ctcggctccg gctgcggata gacgaagcgc tcctcgcgca gcgcatcgac
     1261 gccttgctgc aagatttcgt ccgtgacgac gatgccttgg ctcgtgtaga tctcgcgcag
     1321 ctgctcgagc aactgcgcgt cgcgatcctc ggccgaaagc tcgcgcgcga gcacgagctc
     1381 cttgtgccgc aaagtgtcga cgacgtccat ggcgagcatc agctcgtcga ggggcgcctt
     1441 ctctttgacg accacgtcgt tcatggcagc gctctacgcg cgacgcaagc cgcgctcaac
     1501 gcgtctgcgc gacgagctcg agagcctggc cgctctcaac gagcttcgcc aaacgctgct
     1561 tgccctgctc gaccgcgtcg cgaatctcgg cggcgttctt cgtcgactga tcgcgcatct
     1621 cggcgatgat ctcgcgcgag cgcagctgat agttgacgac ggagtcgacg agctttttta
     1681 cggcatcggc gcgaatcgtc ggtccgtagc cggctttcag agcagcttct tggactttgc
     1741 cgccgatgtc ggccaaatcc tcgaggctct tgctgacgcc ttctttcatc gcatcgagcg
     1801 tctgcgtgct ctcgtgcaag ccccagagcc ccgtgaacga cgcggacaac gccgtcagca
     1861 ccgtctcgtt cgtgccgaag aacgacacgg cctgcttgta gacgcgatcc ttcgcgttgg
     1921 tcgtctgcac gaggcgagcc atgatgacct cgcttgtgtt gtagccgatc gtcaggttgt
     1981 ccgagaggtc cttacagatc tgatagcggt cctcggcacc ctgcacgttg cgcagctctt
     2041 cgtcgcgctt gagctcgagc ttcgcgcgcg cgacgagatc gtcgctgccc gctttctcga
     2101 cggcctcgac ggcgactttg agctgcgctt gcgcggcagc gagctcgcct tcagcgacct
     2161 tcagcacctg caacgccagc acctcggact ctttcagcgc accgcggaaa tcgaggtatg
     2221 cctgcagaat gacttgctcg cgctcgatct ggtccttgga atccttgacg acgtcgaggt
     2281 aaaccttctt gaccttatcg aaccggcccg cgatgtcgcc gcgcgtggct ttctgccaaa
     2341 cgttggccat gcgctcgaac gtgtcgatgc ggccgtcctc gacttgcttg accatcgttt
     2401 tggcgtcgtc gcgaatgctg tcgaagccct cggtgatttt cgcgtaacgc tcgccgagcg
     2461 acatcgacgc gacctgctcg cgcacgactt gattgaacag cgacgcttgc gacagcgtgc
     2521 gcgcgatcgc gacgaccttg tcgttgtcga gatcggaaac tcggttcagc agcgcaacga
     2581 tcggcgcctc ctcgggcttc tcgggcacga ggccgagatc gcgcagccgt gtcattgctt
     2641 tgtcgagata ttgaattgga gttgccatgg cgtgcctcgg tagattggtc tcgattgtgg
     2701 cacggccagt cgtcgtcgtg ttcatgcgct gatccctcca tcaccgctga agcaaacgga
     2761 gagctgattg catccgtcgt gccaatgcgg tcgatccgtg tgtaactgcc tgatctacag
     2821 gctcagttcg gcaacgcaca gcgccgccag ttccaaagcc aacgtatcga aatcgcacaa
     2881 acgttagcgc gtgccgctac gctatcgctc cgccattcat agcagcagga gcgccctacg
     2941 atgccgacag ctcacgagat cgactacgaa attcgcggca acgacatgca gtacgtcgag
     3001 gtcgagctcg accccggcga gagcgccgtc gccgaagcgg gcagcctcat gtacaaggac
     3061 gtcgccgtcg agatgacgac cgtattcggc gacggcggcg gtcagacgag cgggctgatg
     3121 ggcatgctcg tcggcgccgg caaacgtctg ctgaccggcg agagcctgtt catgacggtc
     3181 ttcacgcaca acggcagcgg caaagcgcgc gtcgcttttg cggcgccgta ccccggcacg
     3241 atcctcgcca tggatctgaa aaagctcggc ggcaagctga tctgccagaa agatgcgttc
     3301 cttgcggcgg cgaagggcgt gtcgatcggc atcgcgtttc agaaacgcat catgacgggc
     3361 ttgttcggcg gcgagggctt catcatgcag aagctcgaag gcgacggcat ggtgttcgtg
     3421 cacgccggcg gcacgctcgt cgaacgcgag ctcagagccg gcgaggagct gcacgtcgac
     3481 acaggctgca tcgcggcgct cgcgccgacc gtcgactacg acatcgtcac ggtcggcggc
     3541 gtcaaaagca tgatcttcgg cggcgaaggc gtgttctttg cgcggctgcg cggccccggg
     3601 cacgtgtggc tacagagctt gccgttctcg cgactcgccg gacgaatcat cgcgaacttg
     3661 gggccgacgg gccggcgcac cgacgagggc tcggtgctcg ggccgcttgg cgatattttg
     3721 cgcggcagct gagccgcgca gtgtgcgcgc ccctcgaccg ccgctcgcta gtgccggagc
     3781 tccgcgccgc ccgacggctc ggcgcgcgcg ccgccttcga cgccagcgcc gtcgagagcg
     3841 aacagataga cgctgtcacc gcgcgtcgtg ccggggaaga acgagcccgc cgacagcgcg
     3901 acgatgtact gctggccgtt gcgctcgaac gtgctgacgg ccgcatggat gccggcgtcg
     3961 gtctgatatt cccagagcag cgcgccgttc gacttgtcgt gcgccgtcag acgaccgtcg
     4021 ttgcggccga ggaatagcaa gccgccgccc gtcaccgtgg agccggcgta gcacatctcg
     4081 ccccactgct gctgccacgc gagcttgttg gtgcgaacgt cgacggccgc gatcacgccg
     4141 cgtcgcggca cttgcgagcg cgtgtaatcg ccgccgaagt agcggccgcc cggcgtcgga
     4201 atcgcccatt cgacgccgcc ttccttcgtc gagtacgccg cggcgccgtc cgtcgcgcaa
     4261 acataaaaca aattcgtcgt cgggtcgtac gagctcggcg gccagttcac gccaccggtc
     4321 gcgagcggct taacgaccac gggtttgtcc cagaacggcg tgaagatgcg gccgttgttg
     4381 gtgagcttga agccttccgg ctcgatgtcg atagcgtgcg ggatgacttc gtcgccgcgc
     4441 gggatcggct gcgtcgcggc cgtcgcttgc cgcggctctt gcggcacggc cacctcgtcg
     4501 atgccgacga gcggcttgcc cgtctcgcga tcgaggatgt agacgtagcc ggtcttgccg
     4561 atctcgacga tgcccttgcg cagtcggccg tcgaccgtcg cgtcgaacag cacgaccgga
     4621 tttggcgagt cgtagtccca aagatcgtga tgcacctgct ggaagtgcca gcggtacttg
     4681 cccgtcttcg cctcgattgc gaccatcgag acgctgaaca gattgtcgcc gggccgcacg
     4741 gagccgatca aatcggggcc cgggttgccc gtcgagaagt agacgagccc cagctccgga
     4801 tcgacggccg gcgtctgcca caccgccgcg ccgccgtatt tccacgtgtc gttgttggca
     4861 ggccaggtgt cgtgcccgac ttcgccgggc cccgggatcg tatagaacgt ccagacgagc
     4921 ttgccgtctt ttgcgtcgta ggctttcact cggccgcgcg ccgcgcggtc gccgcccgca
     4981 aagccggtga tgacgaggcc gtcgtaatag agcggcgcgc ccgtgatgct gaagccgtct
     5041 tgattggtct ccgcttggat cgaccagacg accttgcccg tcgcgtgatc gagcgcgacg
     5101 agcttgccgt cgagctgccc cgagaacacc ttgccgtcgc cgatcgcgac gccccggctc
     5161 agccagccgc agcagatgac cgtgatgttc gcgtcgagat gcgcctcgta cgtccaaagg
     5221 atcttgccgt cgtcggcgtc gagcgcgaac acgtcgttgg cacccgtcgg aacgtagatg
     5281 acaccgtcgt agacgatcgg ctgcgcctga cccgagtact tgctctcggt gccagagccg
     5341 ttcaaatgcg tgcgccaagc gggcctcagc gtcgcgacgt tgtccttgtt gatgcgggcc
     5401 agcggcgaat agcgctggtt gtagaggttg ccgccgttcg tgatccagtt tgcggtcggc
     5461 agcgcggtca gctgctgcgc cgtgaatttc ggcggcgccg cagacgcgcg cggcaccggt
     5521 gcccgcgcat tttgcgaggc ggccggttgc gccgatgctg cgccaatcaa gacgccggcc
     5581 gcaccgagca cggcaaaccc aattctgttc atgacggtac ccccgattgc ttgccacgac
     5641 tctagccctt ggcgttccgc acagccagct cgcgggcatc ccgctggagc cggccgctat
     5701 ctcggcctag cccagcgagg tcagtagcgc ttgcagaatt ctctcgtctt gcgggttcgt
     5761 ctcaagatca atgagcgctg ccggcgcgca gctgcgtgcg cgagctcacg cgggactcgc
     5821 cagacaccgt ccttgcggaa cactacgttg cca
//
LOCUS       DATLEE010000685         2838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3561918, whole genome shotgun sequence.
ACCESSION   DATLEE010000685 DATLEE010000000
VERSION     DATLEE010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3561918"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VM692_14975"
     CDS             <1..632
                     /locus_tag="VM692_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HUQ53527.1"
                     /translation="PPVTLQSLLADPAFAIDTDTAIGELLALWGAHYDAARGEPCTQA
                     QEHELRCVFQRRGTLGELRRVNWPTILSLVTADGVEHPVVVSSLGYDHARLVAHGKVY
                     ELPLAELSYYWFGDHLLLWRPGDAPARDLAPGTDDAGVRWLRETLGKLDGQPASDAST
                     KYDEALEQRVRAYQRAHQLTVDGIVGARTQIAMLAELDLADTPSLVEDH"
     gene            634..1287
                     /locus_tag="VM692_14980"
     CDS             634..1287
                     /locus_tag="VM692_14980"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HUQ53528.1"
                     /translation="MSFILDALRKSEHERQRSTLPGLAQVPLAAPAAQLPRWALLLIG
                     LLAAAVLVLGGAWWQSTRSSIETAANVPTVERPVPLPPAPSTPSAAVTTAAAITAPIA
                     TPGPAPEPAAPARQSGLAVAAGPGGTESRPAETPLAADEPALPSAAALAAQGIVVPTM
                     RLELHRFRDEPRDRFVFINGRKYVEGDHLAEGPELVSILPTGAVLVQAGRRFLLVPE"
     gene            complement(1302..1982)
                     /locus_tag="VM692_14985"
     CDS             complement(1302..1982)
                     /locus_tag="VM692_14985"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HUQ53529.1"
                     /translation="MCGRFGLFVTPEVLEEYFSLTESAAALNLPARYNLTPGQAVAVV
                     REHEGRRRVDALQWGLIPFWAKDASIGRKLVNARLDSVAVKPAFREAWTRRRCLIPAS
                     GFYEWSEPIAGRKRPHFIRPGAEPLLAIAGLWERWRSPSGEKLETCVIVTTDANAQLA
                     QVHDRMPLLIPRDAHALWLDSHSSVDDVAKLVDRTPALDLHPVGFGVNDPRKDDETLV
                     APLDAATS"
     gene            complement(1984..2796)
                     /locus_tag="VM692_14990"
     CDS             complement(1984..2796)
                     /locus_tag="VM692_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ53530.1"
                     /translation="MNDATLGSRVSRPNLVKINLTGLATIVRKESRRIVRIWIQTIVP
                     PAITMTLYFIIFGSLVGSRIGSMDGVPYMTFIAPGLIMMAVITNSFANVVSSFFSSKL
                     MLHLEEMLVAPLAHTTIVLGFVVGGVVRGLLVAALVVVVALLFTDLHVVHWFITVTTI
                     VLTAVVFSIAGLFNAIFAKTFDDISIIPTFVLTPLTYLGGVFFSIALLPPFWQKVALV
                     NPILYMVNAFRYGMLGSSDIPIGVAYMIMITFAFALFGAVLLMLNRGSGVRA"
BASE COUNT          503 a          971 c          932 g          432 t
ORIGIN      
        1 ctccgcccgt gacgttgcag tcgctgctcg ccgatcccgc gtttgccatc gacaccgata
       61 ccgcgatcgg cgaactgctc gcgctctggg gcgcgcacta cgatgctgcg cgcggcgagc
      121 cgtgcacgca ggcgcaagag cacgagctgc gttgcgtgtt ccaacgccgc ggcacgctcg
      181 gcgagctgcg gcgcgtcaat tggccgacga tcctttcgct cgtgaccgcc gacggcgtcg
      241 aacatcccgt cgtcgtgtcg tcgctcggct acgatcacgc gcggctcgtc gcccatggca
      301 aggtctacga gctgccgcta gccgagctca gctactattg gttcggcgat catttgctgc
      361 tctggcggcc cggcgacgcg ccggcacgcg acctcgcgcc cggcaccgac gacgccggcg
      421 tgcgctggct gcgtgaaacg ctcggcaagc tcgacggcca gcccgcgagc gacgcgtcga
      481 ctaaatacga cgaggcgctc gagcagcgcg tgcgcgccta tcaacgtgcg caccagctga
      541 cggtcgacgg catcgtcggt gcgcgcacgc aaatcgccat gctcgcggag ctcgacctcg
      601 cggacacacc gtcgctcgtc gaggaccact gacgtgtcgt tcattctcga cgcattgcgc
      661 aaatccgagc acgagcgtca gcgctccacg ttgccggggc tcgcgcaagt gccgctcgcc
      721 gcaccggccg cgcagctgcc gcgctgggca ctgctactga tcggcctgct ggcggccgcc
      781 gtgctggtcc tcggcggcgc gtggtggcag agcacgcgct cgtccatcga gaccgcagca
      841 aacgtaccga ccgtcgaaag gcccgtgccg ctaccgcctg ctccgtcgac accgtcggct
      901 gccgtaacga cggcagcggc gattaccgcg ccgatcgcaa ccccgggccc ggcgccagag
      961 ccagcggcgc ccgcgcgcca aagcggcctc gcggtcgccg cggggcccgg cgggaccgaa
     1021 tcgcgccccg ccgaaacgcc gctcgcggcc gacgagccgg cgctgccgag cgcggccgcc
     1081 ctcgccgccc aaggcatcgt ggtgccgacg atgcggctcg agctgcaccg gttccgcgac
     1141 gagccgcgcg accgtttcgt cttcatcaac gggcgcaagt acgtcgaggg cgaccacctc
     1201 gcggaagggc ccgagctcgt gtcgatcctc ccgacgggcg ccgtgctggt ccaggcgggg
     1261 cgtcgcttcc tgctcgtgcc ggagtagcga gcggggggaa cctagctggt cgccgcgtcg
     1321 agcggtgcga ccagcgtctc gtcgtccttg cgcgggtcgt tgacgccgaa ccctacggga
     1381 tgcaagtcga gcgccggtgt gcggtcgacg agcttcgcga cgtcgtcgac gctgctgtgc
     1441 gagtcgagcc acagcgcgtg cgcgtcgcgt gggatcaaca gcggcatccg atcgtgaact
     1501 tgcgcgagct gcgcgttcgc atccgtcgtc acgatcacgc acgtctcgag cttctcgccg
     1561 cttgggctgc gccagcgctc ccagaggccc gcgatcgcga gcagcggctc ggcgccggga
     1621 cgaatgaaat gcggccgctt gcgaccggcg atcggctcac tccactcgta gaagccgctc
     1681 gcgggaatca agcagcgccg ccgcgtccac gcttcgcgaa acgccggctt cactgcgacg
     1741 ctgtcgagac gcgcattgac gagcttacgg cctatgctcg cgtccttggc ccagaacggt
     1801 atgagccccc attgcagcgc atcgactcgc cgccgaccct cgtgctcgcg cacgacggcg
     1861 acggcctggc cgggcgtcag gttgtagcgt gccggaaggt tcagcgccgc cgcagactca
     1921 gtgaggctga agtactcctc gagaacctcg ggagtgacga acaaaccgaa ccggccgcac
     1981 acgtcacgcc ctgacgccgc tgccgcgatt cagcatgagg agcaccgcgc cgaacagtgc
     2041 gaaagcgaag gtaatcatga tcatgtaagc gacgccgatc ggaatgtccg acgagccgag
     2101 catgccgtaa cgaaacgcgt tgaccatata gaggatcggg ttcacgagcg caaccttctg
     2161 ccagaacggc ggcaacagcg cgatcgagaa aaacacaccg ccgagatagg tcaacggcgt
     2221 cagcacgaac gtcggaatga tcgagatatc gtcgaaagtc ttcgcgaaga tcgcgttgaa
     2281 gagaccggcg attgaaaaca cgacggccgt caacacgatc gtcgtgaccg tgatgaacca
     2341 atgcacgacg tgcagatccg tgaacaacag cgccacgacg acgacgagcg ccgcaacgag
     2401 cagtccgcgc actacgccgc cgacgacgaa gccgagcacg atcgtcgtat gcgccagcgg
     2461 cgcgacgagc atttcctcga gatgcagcat cagcttgctc gagaagaacg acgacacgac
     2521 gttcgcgaac gagttcgtga tgacggccat catgatgagc cccggtgcga tgaacgtcat
     2581 gtacgggacg ccgtccatcg agccgatgcg gctgccgacg aggctgccga agatgatgaa
     2641 gtagagcgtc atcgtgatcg cgggcggcac gatcgtctgg atccagatgc gtacgatgcg
     2701 gcgcgattcc ttgcggacga tggtcgctag gcccgtgaga ttgatcttca cgaggttcgg
     2761 gcggctcacg cgcgaaccca aggtggcgtc gttcatttct tcgcgtcctc cggcttgtct
     2821 tcgacgagcc tgatgaag
//
LOCUS       DATLEE010000686         2352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10994741, whole genome shotgun sequence.
ACCESSION   DATLEE010000686 DATLEE010000000
VERSION     DATLEE010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10994741"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2019)
                     /gene="ftsH"
                     /locus_tag="VM692_14995"
     CDS             complement(<1..2019)
                     /gene="ftsH"
                     /locus_tag="VM692_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HUQ53531.1"
                     /translation="MGWFNDLQRRSHTAPPPDSTAPRRRAPRLPRYIWVWFLGALLVN
                     YFVTRYVVPPADAPLQIPYTVFKEQVAAHNVAEIYAQGATVEGTFSEAITYPAENQER
                     PEDAGRRTRADRSEPRSSTSFTTTVPAFVDPGLESLLIANGVEISAVPIRTGGSPLAT
                     LLLGFGPALLLIGFYVWIYRRASRQGGAGGMGGLMGMGRSQARRYDQESAARVTFDDV
                     AGIDEAENELVEIVDFLKEPERYTRLGGTAPKGVLLVGAPGTGKTLLAKAVAGEAGVP
                     FFSMSAAEFVEMIVGVGAARVRDLFKQARESAPAIIFIDELDAIGRARGQFALGGSNE
                     QEQTLNQILTEMDGFSSRAGVIVLAATNQPDVLDKALLRPGRFDRRVVVNLPDKTGRA
                     AILAVHTRSVPLAADVVLAELAAATPGLSGADLKNMVNEAALLAARREQDEVRSKDFF
                     DALEKIMLGPERPILLSDDDRERIAYHESGHAVLGLLVPGADPVHRVTIVPRGQALGV
                     TYQRPNADRYNYPEAYLRARIVGMLGGRAAEELVYGTKTTGAESDIEQATAIARNMVT
                     RWGMSDKIGLVQLAPRSNPYLGGATAFGADRPYSETTAFTIDAEVQRIIAESYAEACR
                     LLRAHRKVLDALVQALLQRETLDENEILSVTGLPPAAELPTRGVDPLVA"
     gene            2127..>2352
                     /locus_tag="VM692_15000"
     CDS             2127..>2352
                     /locus_tag="VM692_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53532.1"
                     /translation="MRTSRWLLLSLVAVAAAHGYRVGAQDRDDDDDDKGRDDRDVTCS
                     NGLGDVQIRGDLAIASRCTLNGTDVRGNVTL"
BASE COUNT          357 a          805 c          800 g          390 t
ORIGIN      
        1 ggcgacgagc ggatcgactc cgcgcgtggg cagctccgcg gcgggcggca agcccgtcac
       61 ggagaggatt tcgttctcgt cgagcgtctc gcgctgcagc aacgcttgga ccaacgcgtc
      121 gagcaccttg cggtgggcgc gcagcaagcg gcacgcctct gcgtagcttt cggcgatgat
      181 gcgctggacc tcggcgtcga tggtgaacgc cgtcgtctcg ctgtacggcc gatcggcgcc
      241 gaacgccgtg gcgccgccga gatacgggtt gctgcgcggc gcgagctgca cgagcccgat
      301 cttgtcgctc atgccccaac gagtgaccat gttgcgagcg atcgccgtcg cttgctcgat
      361 gtcactctcg gcgcccgtcg tcttcgtgcc gtagacgagc tcctcggctg cgcggccgcc
      421 gagcatgccg acgatgcggg cgcgtaagta cgcttccgga tagttgtagc ggtcggcgtt
      481 cggccgttga tacgtcacgc cgagcgcctg cccgcgcggg acgatcgtga cgcggtgcac
      541 ggggtcggcg cccggcacga ggaggccgag caccgcgtgg ccgctctcgt gatacgcgat
      601 gcgctcgcgg tcgtcgtcgc tgagcaatat tggccgctcc ggccccagca tgatcttctc
      661 gagcgcgtcg aagaaatctt tggagcgcac ttcgtcctgc tcgcggcgcg cggcgagcag
      721 ggccgcctcg ttgaccatgt tcttgagatc tgcgcccgaa agccccggtg tcgcggcggc
      781 gagctctgcc agcacgacgt cggctgcgag cggcacggag cgtgtgtgca cggcgagtat
      841 cgcggcccgc ccggttttgt ccggcagatt gacgacgacg cggcgatcga accgccccgg
      901 ccgcagcagc gccttgtcga gcacgtcggg ctggttcgtg gcggccagca cgatcacgcc
      961 ggcgcggctc gagaagccgt ccatctccgt caggatctga ttcagcgtct gctcttgctc
     1021 gttcgagccg ccgagcgcga actgcccgcg cgcgcggccg atggcgtcga gctcgtcgat
     1081 gaagatgatc gccggcgcgc tctcgcgtgc ctgcttgaag aggtcgcgca cgcgcgccgc
     1141 gccgaccccg acgatcatct cgacgaattc ggccgcgctc atcgagaaga acggcacgcc
     1201 ggcctcgccg gccacggcct tagcgagcag cgtcttgccg gtgcccggcg cgccgacgag
     1261 cagcacgccc ttaggggcgg tgccgccgag gcgcgtatag cgctccggct ccttgaggaa
     1321 gtcgacgatc tcgacgagct cgttctccgc ctcgtcgatg ccggcgacgt cgtcgaacgt
     1381 gacacgcgcg gcgctctcct gatcgtagcg ccgcgcctga ctgcggccca tacccatgag
     1441 gccgcccatg ccgccggcgc cgccctgccg cgaagcgcgg cggtagatcc agacatagaa
     1501 gccgatcaac agcagcgcgg gcccgaagcc gagcagcaac gtcgcaagcg ggctgccgcc
     1561 cgtgcgaatc ggcactgcgc tgatctcgac gccattcgcg atcagcagcg actcgaggcc
     1621 cggatcgacg aatgccggca ccgtcgtcgt gaacgacgtc gacgaccgcg gctcgctgcg
     1681 atcggcgcgg gtgcgtcggc ccgcgtcctc gggtcgctcc tgattctctg ccggataggt
     1741 aatggcctcg ctgaacgtgc cttcgaccgt agcgccctgc gcgtagatct cggcaacgtt
     1801 gtgcgcggca acttgctctt tgaacaccgt gtacggaatc tgcagcggtg catcggccgg
     1861 cggcacgacg tagcgcgtga cgaagtagtt gaccaacagc gcgcctagga accacaccca
     1921 gatgtagcgc ggcagacgcg gcgcgcggcg gcgcggtgcg gtcgaatccg gcggcggcgc
     1981 agtgtgactg cgcctctgta aatcgttgaa ccaacccaac ggctcgcctc ccgtcgctct
     2041 tgccgagaag ggagcaagtt ttatgccacg cgctcgcggg cgcgcgtttt gcatggctta
     2101 gggcctgatt tttcgagcga ttgaccatgc gcacctctcg ctggctgctc ttgagtctcg
     2161 ttgcggttgc cgctgcacac ggctaccgcg tcggcgcgca agaccgagac gatgacgacg
     2221 acgacaaggg tcgcgacgac cgcgacgtca cctgctcgaa cggtctcggc gacgtgcaaa
     2281 tccgcggcga cctcgccatc gcctcgcgtt gcacgctgaa cggcacggac gtcagaggca
     2341 acgtcacgct gt
//
LOCUS       DATLEE010000687         5948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10996091, whole genome shotgun sequence.
ACCESSION   DATLEE010000687 DATLEE010000000
VERSION     DATLEE010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10996091"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VM692_15005"
     CDS             <1..795
                     /locus_tag="VM692_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53533.1"
                     /translation="TLNLSVLDGWWAEAYDSRRGSRNGWGIPPARDEHGDEDRDRQDS
                     TTLYETLQDEVLPLYYARDAKLGYSPEWVRLCKRSMASVLPAFNSERVLRDYLHDFYA
                     PAARQGRAMAVEGFQAARELGAWKEKVRAAWPGVALKLLNSPATEISFAERVTLEVEV
                     TLNGLAPRDVSVECQAKPVLGSELAVPVQGYAENRRPTNGVTYIEGRAVFNKRFEAGS
                     ADAAGVCRYRLEFEPPWAGTLQYEVRAVPAHPHLSHPYELGLLRRL"
     gene            792..2891
                     /gene="glgX"
                     /locus_tag="VM692_15010"
     CDS             792..2891
                     /gene="glgX"
                     /locus_tag="VM692_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HUQ53534.1"
                     /translation="MSDRRRHPRASLAPQPGARLPLGATVQDGGVNFAIFSPRATRVW
                     LRLYRAPADLQPIKELELDAAVHRTYGFWHVFVDGARAGWLYTWRVDGPDEPDEGLRF
                     DEQRELLDPWAKLVSDAVWDREAALAGDHGSALRAEIVARDDYDWEGDRPLRRSLKDA
                     VIYELHVGGFTRHPSARVEHPGTFRGVVEKIPHLLSLGITDVELLPVFAFDTQDVPAA
                     TADIGLHNFWGYSPVSFFAPHRAYAAGPDARGEFRDMVKALHAAGIGVVLDVVLNHTA
                     EGGADGPTIGCKGLVNELFYLLDAEDRTKYLDFTGCGNTISSNQPLVAQWLVEALRYW
                     AVEMHVDGFRFDLAGVLSRDEAGKPLAHAPVISAIEFAPELADVHLIAEAWDAGGVYQ
                     VGSFPGFRWAEWNDRYRDAVRRFVRAEPGRLGEVATRIAGSSDLYAWAGKLPQNGINF
                     VTCHDGFTLCDLVSYDRKHNNANGEHNRDGSDENLSWNSGIEGATDDPEIQRLREQRA
                     RNFIAVLLLSQGVPMLTAGDEVLRTQRGNNNAYCQDSDLSWLDWSFSPAARAMLRFTR
                     ELIALRKRHPSLRRARFLNGHAAGVTAEVQWYGEALEPPEWHDADARVLCFTLAGATP
                     QEGALHVMMNMQSTARYLPLPDASTRNWRRLADTTLVAPDDVVPAGIPVRGDGYPLGP
                     HGIAIFEDSRIVVESPA"
     gene            2888..3469
                     /locus_tag="VM692_15015"
     CDS             2888..3469
                     /locus_tag="VM692_15015"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUQ53535.1"
                     /translation="MKNLLLALLAVAVAPTAFAQGLDDAAYYGLALGTFDYEEGDGQG
                     GAFLSDSVSSYRLMVGYRFMKHLGVEGGYGETGTIRDTASLPVFGGGTVPLTFTSEFK
                     ILTIRLLGVLPFDNGVSLVGGLSYADMRQDIDFQIGTGPQQSTDVDGNEPAYYLGAQY
                     DWDRFAVRLGYEKYDLQGDVDANETMITFFYKL"
     gene            complement(3485..5686)
                     /gene="glgB"
                     /locus_tag="VM692_15020"
     CDS             complement(3485..5686)
                     /gene="glgB"
                     /locus_tag="VM692_15020"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HUQ53536.1"
                     /translation="MTPPSERVAKADMTPRDPEIERLLHGRLHEPRHVLGAHDRGADE
                     VVVRILRPDATRIRLVDPAVELVRIPGTALFEWTGPKASITKPYRLRWESHDGRWHEG
                     YDPYSFPLEIDDDDLTRFASGSHIHAHRFLGAHVRTVAGVRGVRFAVWAPNAERVSVV
                     GPFNHWDGRYNPMSARGGSGVWELFLPELPARELYKYEISARDTRELKLKSDPYGAAF
                     EMRPATASVTTSPTTFAWNDAAWLEQRARRDWLKEPISIYEVHLGSWRRNAAGHFLNY
                     RELAEQLGHYVKDLGFTHVELLPITEHPFDDSWGYQCTGYFAPTSRHGSPDELRAFVA
                     ALHALGLGVLLDWVPGHFPKDDFALRRFDGSALYEYGDPQKGEHPDWDTLVFNYTRHE
                     VQSFLLSSAICWLEDFHFDGLRVDAVASMLYLDYSRKPHQWTPNQHGGNENLEAIEFL
                     KRLNQTTHVTCPGTITVAEESTAWPKVSRPVYDGGLGFTFKWNMGWMHDTLSYMAHDP
                     IHRRFHHNLLTFGPVYAFTENFVLPLSHDEVVHGKRSLLDKMPGDEWQRFANLRLALT
                     FQWTYPGKKLLFMGGEFGQPREWSHHGGLPWHLADLPRHSGVHALVRDLNRMYRSTPA
                     LHRLDHESDGFAWLSWQDDAQSVVSFLRKAGDEHVVVLLNFTPIPRDGYRVGVPRLGR
                     YRAVLNSDSQYYGGSNVGNDVAEAEPTPCMGQPYSIVMRLPPLGGIVLAPE"
     gene            5741..>5948
                     /locus_tag="VM692_15025"
     CDS             5741..>5948
                     /locus_tag="VM692_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019591638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HUQ53537.1"
                     /translation="MRQIPRRRLVSQLTRSTVAVVMAGGRGQRLMQLTDHRAKPATPF
                     GGKYRIIDFSLSNCVNSGIRQIFIL"
BASE COUNT          988 a         1959 c         1999 g         1002 t
ORIGIN      
        1 acactgaact tgagcgtgct ggacggctgg tgggccgagg cttacgacag ccgccgcggc
       61 tcgaggaacg gctggggcat tccgccggcg cgcgacgagc acggcgacga ggaccgcgac
      121 cggcaggact cgacgacgct ctatgaaacg ctgcaggacg aggtcctgcc gctctactac
      181 gcgcgtgacg cgaagctcgg ctactcgccg gagtgggtgc ggctctgcaa gcgctcgatg
      241 gcgagcgtgc tgccggcgtt caacagcgag cgcgtgctgc gcgattacct gcacgatttc
      301 tacgcgccgg cggcgcgtca aggccgcgcc atggccgttg aaggcttcca ggccgcgcgg
      361 gagctcggag cgtggaagga gaaggtccgg gcggcttggc ccggagtcgc gctgaagctg
      421 ctgaattcgc cggcgaccga aatctcgttc gccgagcgcg tgacgctcga ggtcgaggtc
      481 acgctgaacg gcctggcgcc gcgcgacgtg tccgtcgagt gccaggcgaa accggtgttg
      541 ggctcggagc tcgccgtgcc cgtccagggc tacgccgaga atcgtcggcc gacgaatggc
      601 gtcacgtaca tcgagggccg cgccgtgttc aacaaacgct tcgaagccgg ctccgccgac
      661 gccgcgggag tgtgccgcta caggctcgag ttcgagccgc cgtgggccgg gaccctccag
      721 tacgaagtcc gcgccgtgcc ggctcatccg cacttgtcgc atccgtacga gctcggcttg
      781 ctgcgccggc tttgagcgac cgtcgccgcc atccacgcgc gagccttgcg ccgcagcccg
      841 gtgcacggct gccgcttggc gccaccgtgc aagacggcgg tgtgaatttc gcgatcttca
      901 gtccacgcgc gacgcgcgtc tggctgcgtc tctatcgcgc acccgccgac ttgcagccga
      961 tcaaggagct cgagctcgac gcggccgtgc accgaaccta tgggttttgg cacgtcttcg
     1021 tcgacggcgc gcgagcgggc tggctttaca cgtggcgcgt cgacggcccc gatgagccgg
     1081 acgagggcct gcgtttcgat gagcagcgcg agctgctcga tccgtgggcc aagctcgtca
     1141 gcgacgcggt ttgggaccgc gaggcggcgc tcgccggcga ccacgggtcc gcgctgcgcg
     1201 ccgagatcgt cgcccgcgac gactacgact gggagggcga tcgcccgctg cgccgctcgc
     1261 tcaaggacgc cgtcatctac gagctgcacg tcggcggctt cactcggcac ccttcggcgc
     1321 gggtcgagca tccgggcacg tttcgcggcg tcgtggagaa aattccgcat ctgctgtcgc
     1381 tcggcatcac cgacgtcgag ctgctgcccg tgtttgcctt cgacacgcaa gacgtgccgg
     1441 ccgcgacggc cgacatcggg ctgcacaact tctggggtta cagccccgtg agctttttcg
     1501 cgccgcaccg cgcctatgcc gccggccccg acgcgcgcgg cgagttccgc gacatggtca
     1561 aggcgctgca tgccgcgggc atcggcgtag tcctcgacgt cgtgctgaac cacaccgccg
     1621 aaggcggcgc cgacggcccg acgatcggct gcaaggggct cgtcaacgag ctgttctatt
     1681 tgctcgacgc cgaagaccgc acgaagtacc tcgacttcac gggctgcggt aacacgatca
     1741 gctcgaatca gccgctcgtt gcacagtggc tcgtcgaggc actgcgttac tgggcggtcg
     1801 agatgcacgt cgacggcttc cgcttcgact tggccggcgt attgtcgcgc gacgaagccg
     1861 gcaagccgct cgcgcatgcg cccgtcatct ccgcgatcga gttcgcgccc gagctcgccg
     1921 acgtgcactt gatcgccgag gcatgggatg ccggcggcgt ctaccaagtg ggctcgtttc
     1981 caggcttccg ctgggccgag tggaacgatc gctatcgcga cgccgtgcgc cggttcgtgc
     2041 gcgccgagcc cggccggctc ggtgaggtcg cgacgcgcat cgcgggcagc agcgatctgt
     2101 atgcgtgggc aggcaagctg ccgcagaacg gcatcaactt cgtgacgtgc cacgacggct
     2161 tcacgctctg cgacctcgtc agctacgacc gcaagcacaa caacgcaaac ggcgagcaca
     2221 accgcgacgg cagcgacgag aacctgagct ggaattccgg catcgaaggt gcgacggacg
     2281 atccggagat tcagcggctg cgcgagcaac gcgcgcgcaa cttcatcgcc gtgctgctgc
     2341 tgagtcaggg cgtgccgatg ttgacggccg gcgacgaggt cctgcgcact cagcgcggca
     2401 acaacaacgc gtattgccag gacagcgatc tcagctggct cgattggtcg ttctcgccgg
     2461 cggcgcgcgc gatgctgcgc ttcacgcgcg agctgatcgc gctgcgcaag cggcatccga
     2521 gcttgcggcg cgcacggttc ctgaacggcc acgcggccgg cgtcacggcg gaggtccaat
     2581 ggtacggcga agcactcgag ccgccggagt ggcacgacgc cgacgcgcgc gtgctgtgct
     2641 tcacactggc cggtgcgacg ccgcaagagg gcgcgctgca cgtcatgatg aacatgcagt
     2701 cgacggcccg gtacttaccg ttaccggacg cgtcgacacg gaattggcgt aggctcgccg
     2761 atacgacgct cgtggctccg gacgacgtcg tgcccgcagg tatccccgtg cgcggcgacg
     2821 gttatccgct cgggccgcac ggcatcgcca tcttcgagga cagtcgaatc gtggtagagt
     2881 cgcccgcatg aaaaatctac tgctcgcgtt actcgccgtc gcagtggctc ccaccgcatt
     2941 cgctcaaggc ttggacgacg ccgcgtacta cggtctcgcg ctcggcacct tcgattacga
     3001 ggaaggagac ggacagggcg gcgcattctt gagcgacagc gtgagctcgt accggctcat
     3061 ggtcggctac cgcttcatga agcacctcgg agtcgaaggc ggctacggcg agacgggcac
     3121 catccgtgac acggcgagtc tgccggtctt cggcggcggc acggtgccgc tgacgttcac
     3181 ctcggagttc aagatcctga cgatccgttt gctgggcgtg ctgccgttcg ataacggcgt
     3241 gtcgctcgtc ggcggtttga gctatgccga catgcggcag gacatcgact tccagatcgg
     3301 cacgggtccg cagcagtcga cggacgtcga cggcaacgag ccggcgtact acctcggcgc
     3361 gcaatacgac tgggatcgat tcgcggtccg cctcggctat gaaaaatacg atctccaggg
     3421 cgacgtcgac gccaacgaga cgatgatcac gttcttctac aagctctagt cgctcgggct
     3481 tcagtcactc cggcgcgagc acgatgccgc cgagcggcgg cagtctcatc acgatcgagt
     3541 aaggctggcc catgcaaggc gtcggctccg cttcggcgac gtcgttgccg acgttcgagc
     3601 cgccgtagta ctgcgagtcg gagttcagga ccgcacggta acggccgagc cgcggcacgc
     3661 cgacgcgata gccgtcgcgc ggaatcggcg tgaaattcaa caggacgacg acgtgctcgt
     3721 cgccggcttt gcgcaagaag ctcacgacgg actgcgcgtc gtcttgccac gacagccaag
     3781 cgaagccgtc gctttcatga tcgagccggt gcagcgccgg cgtgctgcgg tacatgcgat
     3841 tcaggtcgcg cacgagcgcg tggactccgc tgtggcgcgg caggtccgcg aggtgccacg
     3901 gcaagccgcc gtgatggctc cactcgcgcg gctggccgaa ctcgccgccc atgaacagca
     3961 gcttcttgcc gggataggtc cactggaacg tcagcgcgag ccgcaagttc gcgaagcgct
     4021 gccactcgtc gcccggcatc ttgtcgagca gcgagcgctt gccgtgcacg acctcgtcgt
     4081 gcgacagcgg cagcacgaag ttttccgtga aggcgtacac agggccgaac gtcagcagat
     4141 tgtggtggaa gcgccggtgg atcgggtcgt gcgccatgta gctcaacgtg tcgtgcatcc
     4201 accccatgtt ccatttgaac gtgaagccga ggccgccgtc atagacgggg cgtgaaacct
     4261 tcggccacgc tgtcgactct tcggcgacgg tgatcgtgcc tgggcacgtg acatgcgtcg
     4321 tctggttcaa gcgcttcaag aactcgatcg cttcgaggtt ctcgttgccg ccgtgctggt
     4381 tcggcgtcca ctgatgcggc ttgcgcgaat agtcgaggta cagcatcgac gccacggcgt
     4441 cgacgcgcag gccgtcgaaa tgaaagtcct cgagccaaca gatcgcgctc gacagcagga
     4501 agctttggac ttcgtggcgc gtgtagttga acacgagagt gtcccagtcg gggtgctcgc
     4561 ccttctgcgg gtcaccgtac tcgtacaacg ccgacccgtc gaaccgccgc agcgcgaagt
     4621 cgtctttcgg gaagtggccc ggaacccagt cgagcagcac gcccagcccg agcgcgtgca
     4681 gcgccgcaac gaacgcgcgc agctcgtcgg gcgagccgtg cctgctcgtc ggcgcgaagt
     4741 aacccgtgca ctggtacccc cacgagtcgt cgaacggatg ctccgtgatc ggcagtagct
     4801 ccacgtgcgt gaagcccagg tccttgacgt agtggccgag ctgctcggcg agctcgcgat
     4861 agttcaagaa atggcccgcg gcgttgcggc gccacgagcc caggtgcacc tcgtagatcg
     4921 agatcggttc cttgagccaa tcgcgtcggg cgcgctgctc gagccacgct gcatcgttcc
     4981 acgcgaacgt cgtcggcgac gtcgtcacgc tcgccgtcgc gggccgcatc tcgaaagcgg
     5041 cgccgtacgg atcgcttttg agcttgagct cgcgcgtgtc gcgggccgag atctcgtact
     5101 tgtagagctc gcgggccggg agctccggca ggaacagctc ccaaacgccc gagccgccgc
     5161 gcgcgctcat cgggttgtac cgtccgtccc aatgattgaa cgggccgacg acgctgacgc
     5221 gttcggcgtt cggcgcccag acggcaaagc gaaccccgcg tacgccggcg acggtgcgga
     5281 catgcgcgcc gagaaagcga tgcgcgtgaa tgtggcttcc cgacgcgaag cgcgtaaggt
     5341 cgtcgtcgtc gatctcgagc ggaaacgagt aggggtcgta gccttcgtgc cagcggccgt
     5401 cgtgcgattc ccagcgcagt cgatacggct tcgtgatgct ggctttgggc ccggtccatt
     5461 cgaacagcgc cgtgccggga atgcgtacga gctcgacggc cgggtcgacc aaacggatcc
     5521 gcgttgcatc gggccgcaga atgcgcacga cgacttcgtc ggcgccgcgg tcgtgcgcgc
     5581 cgagcacatg gcgcggttcg tgcagcctgc cgtgcagaag gcgctcgatt tccggatcgc
     5641 gcggcgtcat atcggccttc gcgacccgct cgctgggcgg cgtcatacct ggtgctcttg
     5701 gttagacagg cgcagtcctg tagtagtagc gtgtagcaaa atgcgacaaa tcccccgccg
     5761 acgcctcgtc agccaattga ctcgcagtac cgtcgccgtc gttatggcgg ggggccgagg
     5821 tcaacggctc atgcaattga cggaccatcg cgccaagccg gccacgccgt tcgggggcaa
     5881 gtaccgcatc atcgacttct ctttgtcgaa ctgcgtcaac tccggcatcc gtcagatctt
     5941 catcctga
//
LOCUS       DATLEE010000688         3055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11005996, whole genome shotgun sequence.
ACCESSION   DATLEE010000688 DATLEE010000000
VERSION     DATLEE010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11005996"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VM692_15030"
     CDS             complement(<1..1075)
                     /locus_tag="VM692_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53538.1"
                     /translation="MTLANRRLATLAVFAGALAYFLLFRRYGFQIEDEGTLLFQLDRA
                     ARGELPYTDFHTGYTPGFFAAGAALLRLAHWDVASLRTVLAVLQAGVAASLYALAAPL
                     TGAALAVVAPLAFVAFLPVWPGDFASFNVPYPAWFANVAWMATALAMTSFARRGRLAP
                     LFVAGLAAGAAFAIKPNAGAFALAAASWTVAMTATSRTALDRAAAIGSCAVMLAGVWF
                     AFGLTVRSIDAAVHLPPCIAIAVLAAWRARGALASPSHPGAAAACAALVAGFALPTIA
                     WALPTLARLGTDGFLREVLLIGSNAAGIYWLAHPAPQLWAAVSIGGALAVAGAGHLVA
                     RGRVRPAPLLAAASLAVVAALLFL"
     gene            1406..2527
                     /locus_tag="VM692_15035"
     CDS             1406..2527
                     /locus_tag="VM692_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53539.1"
                     /translation="MLLLAGATALPMLAIPLTGGMHPDQTAILSLAGLLGFFGGPAHV
                     AMTSWFYTDPVAREHFRAHPWRYGFIPAALVVGTTAAYAIWQESQPTRWINFGFTVWL
                     LWHYQKQNWGIHSFVTRVMSGGSASRLEEWIVRLAVVGGIVGGIRSAGFGGGTPVEAY
                     AGAAFATGAAITLALPLLIAVALATVPALRAEPLRIASLCGIAAFFLPVFAFRNPNNA
                     VLPYALAHGLQYAVFMSYVGRSTRAQKSEVWRPGLATMAVCLLAVGWMLRSFGNVGQV
                     REWNLLPLFGLTLGVTMAHFVIDAGIWRLRDEFPRRYVGAAFPFLKPRTAGVAAVQVC
                     DERPVADTADERPVAEPADERPVAEPAAERPFAMARHGR"
     gene            2545..>3055
                     /locus_tag="VM692_15040"
     CDS             2545..>3055
                     /locus_tag="VM692_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53540.1"
                     /translation="MSPLRDASRRFAGRILNRGAAHATLLFDVTFWLGAALFAVELAR
                     RSITLGDEGYLMNQSLDLASGKVPYRDFDLFVTPGAWLLNALVFTVFGPSIVVTRYVA
                     ALSLVAAMAVAAGIVRETLRADLRRPSRGRWSRFAAALTGMLAVWAFPAWTISFYSPY
                     ATLAAMTGLL"
BASE COUNT          421 a         1112 c         1081 g          441 t
ORIGIN      
        1 gcaggaacag cagcgccgcg acgactgcga gcgatgccgc cgcaagcagc ggtgccggcc
       61 ggacgcgacc tctcgcgacg agatggcctg cacccgcaac ggccagcgcg ccgccgatcg
      121 acacggccgc ccacagctgc ggcgccggat gggcgagcca gtagatgccg gccgcgttcg
      181 acccgatcag caggacttcg cgaaggaagc cgtccgtgcc gagccgcgcg agcgtcggca
      241 gcgcccacgc gatcgtcgga agcgcgaagc cggcgacgag cgcggcgcaa gcggcggccg
      301 caccgggatg cgatggcgat gcgagcgcac cgcgcgcacg ccacgctgcg agcacggcga
      361 tcgcgatgca cggcggcagg tgcacggccg catcgatcga ccgcacggtc aggccgaacg
      421 cgaaccacac gccggcgagc atcaccgcgc acgagccgat cgcggccgca cgatcgagcg
      481 ccgtcctcga cgtcgccgtc atcgcgaccg tccagctcgc cgccgcgagc gcgaaagcgc
      541 cggcgttcgg cttgatcgca aacgccgcgc cggccgcgag cccggccacg aagagcggtg
      601 cgagccgtcc gcggcgcgcg aacgacgtca tcgcgagcgc cgtcgccatc caagcgacgt
      661 tggcgaacca cgcggggtag ggcacgttga acgacgcgaa atcgcccggc cacaccggca
      721 ggaacgcgac gaacgcgagc ggagccacga cggcgagcgc ggcgccggtg agcggcgctg
      781 ccagcgcgta gagcgatgcc gcgaccccgg cttgcagcac cgcgagcacc gtgcgcagcg
      841 acgcgacgtc ccagtgggcg aggcgcagca gcgcggcgcc cgccgcaaag aagcccggcg
      901 tgtagccggt atggaagtcg gtgtacggaa gctcgccgcg cgctgcgcgg tcgagctgga
      961 agagcagggt gccttcgtcc tcgatctgga agccgtaacg gcggaacagg aggaagtagg
     1021 cgagggcgcc cgcgaagacg gcgagcgtcg cgagtcggcg attcgcgaga gtcatgcgcc
     1081 ccgttggccg cgcgccgctg cgcgcttcag ccgggtggcc cacagttgcg cagccccggg
     1141 ccgtcctcaa gccgcgcccg cgacttcggc tgccgcgacg ggcgatttcc cgagacgaca
     1201 gggggcggct cgcgcgctat agtcggtgct tcgcttcgcc gcggcgcgag atcggtgcca
     1261 gccaccgctg ccgcgggcgg ttccggggac ggtgcggcgc ggcagtgggc accggtgcca
     1321 ggcaccacga agcgacggag cgaagcagct gatcaccacc ctgcgagacc gctgcctcgg
     1381 cagcgacgcg cgcttccggc gcttcgtgct gctgctcgcg ggcgcgacgg ctctgccgat
     1441 gctcgcgatc ccgctgacgg gcggcatgca ccccgaccag acggcgatcc tctccctcgc
     1501 gggccttctc ggcttcttcg gcggcccggc ccacgtcgcg atgacgagct ggttctacac
     1561 cgaccccgtc gcgcgcgagc acttccgagc ccacccgtgg cgctacggct tcatccccgc
     1621 ggcgctcgtc gtcggcacca cggcggccta cgcgatctgg caggagagcc agccgacccg
     1681 ctggatcaac ttcggcttca cggtctggct gctgtggcac taccagaagc agaactgggg
     1741 cattcattcg ttcgtgacgc gcgtgatgag cggcggatcg gcgtccaggc tcgaagagtg
     1801 gatcgtccgc ctcgccgtcg tcggcgggat cgtcggcggg atccgcagcg ccggcttcgg
     1861 cggcggcacg ccggtcgaag cgtacgccgg cgccgcgttc gcgaccggcg ccgcgatcac
     1921 gctcgcgctt ccgctgctca tcgccgtcgc gctcgcgacc gtgccggcgc tgcgcgcgga
     1981 gccgctgcgg atcgcgtcgc tctgcggcat cgcagcgttc ttcctgccgg tcttcgcgtt
     2041 caggaatccg aacaatgcgg tgctgcctta tgccctcgcg cacgggctgc agtacgccgt
     2101 gttcatgagc tacgtcggcc gctccacgcg cgcgcagaag tccgaggtgt ggcgccccgg
     2161 tctcgcgacg atggccgtgt gtcttctcgc cgtcggctgg atgctgcgct cgttcggcaa
     2221 cgtcgggcag gttcgcgagt ggaacctgct gccgctgttc gggctcacgc tcggcgtcac
     2281 gatggcccac ttcgtcatcg acgccggcat ctggcggctt cgcgacgagt ttccgcgccg
     2341 ctacgtcggt gccgcgtttc ccttcctgaa gccgcggacg gcgggcgtcg cggccgtgca
     2401 ggtctgtgat gagcggcctg ttgccgatac ggccgacgag cggccggttg ctgaaccggc
     2461 cgacgagcgg cctgttgccg aaccggccgc tgagcggcct tttgcgatgg cacggcacgg
     2521 gcgctgacgc ccgctttccg aagcatgtct ccccttcgcg acgcgtcacg acggttcgcc
     2581 ggccggatcc tcaaccgcgg cgccgcgcac gcgacgctgc tgttcgacgt gacgttctgg
     2641 ctcggtgccg ccctgttcgc cgtcgagctc gcgcggcgat cgatcacgct cggtgacgaa
     2701 ggctacctga tgaaccagtc gctcgacctc gcgtcgggca aggttccgta ccgcgacttc
     2761 gatctcttcg tcacgccggg tgcgtggctg ctcaacgcac tcgtcttcac ggtcttcgga
     2821 ccgtccatcg tcgtgacccg ctacgtcgcc gcactgtcgc tcgttgccgc gatggccgtc
     2881 gccgcaggca tcgtgcgcga gacgctgcgc gccgacctgc gccggccgtc gcgcggccgc
     2941 tggagccgct tcgctgccgc gctcaccggc atgctcgccg tctgggcctt tccggcgtgg
     3001 acgatttcgt tctactcgcc gtacgcgacg ctcgccgcga tgacggggct gctct
//
LOCUS       DATLEE010000689         2110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3575700, whole genome shotgun sequence.
ACCESSION   DATLEE010000689 DATLEE010000000
VERSION     DATLEE010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3575700"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..755)
                     /locus_tag="VM692_15045"
     CDS             complement(54..755)
                     /locus_tag="VM692_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HUQ53541.1"
                     /translation="MNNVAVRNIDRADAKVVQALGEHGTATVHEAQGRTGLLQPHMRP
                     IYSGARIGGSAITVLLHPGDNWMLHVAVELARPGDIMVVAVSAPNSDGMFGDLLATSF
                     KARGVLGLVIDAGVRDVKDLTEMRFPVWSRAISAKGTVKATVGSVNVPIVCAGMLVNP
                     GDAIVADDDGVVVVPRQKAPAVLEASKQRVANEEQKRAKLAAGELGLDIYKMREPLAK
                     AGLVYYDRLSDVDKG"
     gene            835..2007
                     /locus_tag="VM692_15050"
     CDS             835..2007
                     /locus_tag="VM692_15050"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HUQ53542.1"
                     /translation="MTEAAPLTGIKVVDMTEALAGPYCAMLLGDFGADVIKVERPGDG
                     DQARGWGPPFLEGESAYFLSVNRNKRSIELDTKQPDDLSVLQTLIGRADVFLTNNPKL
                     ESLARSSLDPQTLRARHPRLVYGAISGYGHTGPKANRGGYDIIAQGEAGLMALTGPVD
                     GGPSRFPTPMADISGGIYTCMGVLAALYARDNRANGSGTGQLIDVALVDAQLSWLANI
                     GASYFATGKRPARLGNAHATVTPYQPIRARDKTMIIAVGNERLWAKFCKIIGAEGTLL
                     NDPRFATNAERNVHRTELIALLEQILVRRTAAEWVEDFVAAGIPAGPINFPDETLTDE
                     HVLARRMIVELEHPLIGVVRSIAAPMRFESNGPTYRRYPPRLGEHNAEIRRNAATW"
BASE COUNT          353 a          705 c          704 g          348 t
ORIGIN      
        1 gaggcccgtt ccccgagccg cgtttcctgt cgtcggttcc cgactgacgc gaatcagcct
       61 ttgtcgacgt cgctgagccg gtcgtagtag acgagcccgg ctttcgcgag cggctcgcgc
      121 atcttgtaga tgtcgaggcc gagctcgccg gccgcgagct tggcgcgctt ctgctcctcg
      181 ttcgcgacac gttgcttgct cgcctccagg acggcggggg ccttctgccg cggcacgacg
      241 accacgccgt cgtcgtcggc gacgatggcg tcgcccggat tgacgagcat gccggcgcac
      301 acgatcggta cgttgacgga gccgacggtc gccttgacgg tgccttttgc cgatatcgcg
      361 cggctccaga cggggaaacg catctcggtc agatctttga cgtcgcggac gccggcgtct
      421 atgacgaggc cgagcacgcc gcgcgctttg aacgaagtcg ccaacaagtc gccgaacatg
      481 ccgtcggagt ttggcgcact gacggcgacg accatgatgt cgccgggccg tgccagctcg
      541 acggcgacgt gcaacatcca attgtcgccg gggtgcagca agaccgtgat cgcgctgccg
      601 ccgattcgcg cgccgctgta gatcgggcgc atgtgcggct gcagcagtcc cgtgcggcct
      661 tgcgcctcgt gcacggtcgc cgtgccgtgc tcgccgagtg cttgcacgac cttggcgtcc
      721 gcgcggtcga tgttgcggac tgcgacgttg ttgatcaccc ctgctctcct gtcaccgcgg
      781 aaaaagcggc gcttatacta gccgccgatc attagagcac aggccggaga gatcgtgacc
      841 gaagctgcgc cgctgacagg aatcaaagtc gtcgacatga cggaggcgct cgccggcccg
      901 tactgcgcga tgttgctcgg cgacttcggc gccgacgtca tcaaagtcga gcggccgggt
      961 gacggcgatc aagcgcgagg ttggggcccg ccgttcctcg aaggcgagag cgcgtatttt
     1021 ctgtccgtca atcgcaacaa gcgcagcatc gagctcgaca cgaagcaacc cgacgaccta
     1081 agcgtgctgc aaacgctgat cggccgggcc gacgtgttcc tgacgaacaa tccgaagctc
     1141 gagtcgctcg cgcgctcgag cctcgacccg cagacgttgc gagcgcggca cccgcggctc
     1201 gtgtacggcg cgatcagcgg ctatgggcac acggggccga aggccaatcg cggcggctac
     1261 gacatcatcg cgcaaggcga agccgggttg atggcgctga cgggccccgt cgacggcggg
     1321 ccgagccgct ttcctacgcc gatggccgac atctcgggcg gcatttatac gtgcatgggc
     1381 gtgctagccg cgctctacgc gcgcgacaac cgcgcgaacg gcagcggcac gggccagctc
     1441 atcgacgtcg cgctcgtcga cgcgcagctg agctggctcg ccaacatcgg ggcgagctat
     1501 ttcgcgacag gcaaacggcc cgcgcggctc ggtaacgcgc atgcgaccgt cacgccgtat
     1561 cagccgattc gtgcacgcga caagacgatg atcatcgcgg tcggcaacga acggctgtgg
     1621 gcgaagttct gcaagattat cggcgccgag ggcacgctgc tgaacgatcc gcggttcgcg
     1681 acgaatgccg aacgcaacgt ccatcgaacc gagctcatcg cgctgctcga gcagatcctc
     1741 gtgcggcgta ccgcggccga atgggtcgag gacttcgtcg ccgccggaat ccccgcgggg
     1801 cccatcaact ttcccgacga gacgttgacc gacgagcacg tgctcgcgcg gcgcatgatc
     1861 gtcgagctcg agcatccgct gatcggcgtc gtgcgatcga tcgcggcccc gatgcgcttc
     1921 gagtcgaacg gcccgacgta tcgccgctac ccgccgcgct tgggcgagca caacgcggag
     1981 atccgccgca acgcggcgac gtggtgagcc ctaggcgtta gcgctcccag acgttctgca
     2041 gatacgcgcg gaagctttcg ccgagctccg ggtgccgcaa gccgtattcg acggttgcgc
     2101 gcaggtagcc
//
LOCUS       DATLEE010000690         2884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7919817, whole genome shotgun sequence.
ACCESSION   DATLEE010000690 DATLEE010000000
VERSION     DATLEE010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7919817"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VM692_15055"
     CDS             complement(<1..588)
                     /locus_tag="VM692_15055"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HUQ53543.1"
                     /translation="MNKTTFTALTVLLAYAGCADAQTPSRARRAAAPKVVLIIGDGMD
                     QNQISIARNYLVGQDGRLTLDTLPVRSAVQMQTVLEEDPKVPEYVPDSANTASSMASG
                     IVTSTGRIGTTPGDDKDAPTIMELAAAAGFGTGIVTTSSVTDATPASFVAHVNQRLCE
                     SPRSMVARNAANPRFSSDCSADLKANGGKGSIAEQI"
     gene            complement(594..2633)
                     /locus_tag="VM692_15060"
     CDS             complement(594..2633)
                     /locus_tag="VM692_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HUQ53544.1"
                     /translation="MLSAIVAAVTAADQGAEPPGSVRPAGDGNGFRVEVERFADVRIL
                     RYRVPGFEQLDARTKELLYYLYEAALCGRDIIYDQKYRYNLAVRRTLELIVRHYSGDR
                     ETDAFRALALYLKRVWFANGLHHHYAHDKFEPGFSPAAFADFVRKTPAEFPVRRGQSL
                     DDFISELTPVLFDPTVDAKLVSKSADRDVLTSSAVNFYSGLTQREVEVFYAQQAVAGD
                     DAPVSLGLNSRLVKVDGKVVEDVQRIGGRYTQAIERMVAWLEKAAGCAENDAQRNALT
                     KLIAFYRSGSLRDWDDYNIAWLRDTESHVDLIHGFIEVYNDPLGMRGSFESVVSFRDP
                     EATRRIGAIASAAQWFEDHMPFLERHKKPNVTGITGKVITVVIEAGDAAPTTPIGINL
                     PNSDWIRRAHGSKSVSLANVVAAYNAVRGGADREFSYDAAEIARNERHGELAAALHID
                     MHEVIGHGSGQIEPGVGPLHETLKNYGSTLEEARADLVALYYAVDPKLVELGVMPSIE
                     VGYAAYDHFVRNALLQQLNRVAAGKDLEEDHMRNRQLIAAWAYEQGAADNVIEKRTRD
                     GKTYFVVNDYAKLRSLFGVLLRELQRIKSTGDYAAIRALVERYGVEVDQELRAEVHAR
                     YAELDIPAYTGFLGPRLVPVREGETIVDVRIEYPDDFAAQMLEYADRYSLLPAWN"
     gene            2667..>2884
                     /locus_tag="VM692_15065"
     CDS             2667..>2884
                     /locus_tag="VM692_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53545.1"
                     /translation="MRTVELRKPQDHAISLHVAAALVLLATLTLSPARFAAAQGAVPC
                     TAIANDAERLACYDRALRGAPPPAASPAP"
BASE COUNT          480 a          978 c          926 g          500 t
ORIGIN      
        1 gatctgttcg gcgatcgagc ccttgccgcc gttggccttg aggtcggccg agcagtccga
       61 cgagaaccgc ggattggcgg cgttgcgcgc gaccatcgag cgcgggctct cgcagagccg
      121 ctggttgacg tgcgcgacga acgaagccgg cgtcgcgtcc gtcacgctcg acgtcgtgac
      181 gatgccggtg ccaaagcccg cggccgccgc gagctccatg atcgtcggcg catccttgtc
      241 gtcgccggga gtcgtgccga tgcggcccgt gctcgtcacg atgccgctcg ccatcgagct
      301 cgccgtgttc gcagagtccg gcacgtactc gggcaccttg gggtcctcct cgagcaccgt
      361 ctgcatctgc accgcgctgc gcacgggcag cgtgtccagc gtcagacggc cgtcttgacc
      421 cacgagatag ttacgcgcga tcgagatctg gttctggtcc atgccgtcgc cgatgatcag
      481 gacgactttc ggcgccgccg cgcggcgagc acgcgacggc gtttgcgcat cggcacaacc
      541 ggcgtaagcg agcagcaccg tcagcgcggt aaaagtcgtt ttgttcatgt gcgtcagttc
      601 caagcaggca aaagcgagta gcgatccgcg tactcgagca tctgcgccgc aaaatcgtcg
      661 ggatactcga tgcgcacgtc cacgatcgtc tcgccctcac ggaccggcac gagacgcggc
      721 cccaagaagc ccgtgtaggc aggaatgtcg agctccgcgt agcgcgcgtg cacctcggca
      781 cgcaactcct ggtcgacctc gacgccgtag cgctcgacga gcgcgcggat cgccgcgtag
      841 tcgcccgtcg acttgatgcg ctgcagctcg cgcaacagca cgccgaacag cgaccgcagc
      901 ttcgcgtaat cgttgacgac gaaatacgtc ttaccgtcgc gcgtgcgttt ctcgatgacg
      961 ttgtccgccg cgccctgctc gtaggcccag gccgcaatca actgccgatt gcgcatgtga
     1021 tcctcctcga gatccttgcc ggccgcgacg cgattcagtt gctgcagcag ggcgttgcgg
     1081 acgaagtggt cgtacgccgc gtaaccgact tcgatggacg gcatgacgcc gagctcgacg
     1141 agcttcggat cgaccgcgta atagagcgcc acgaggtctg cacgcgcttc ttcgagcgtc
     1201 gagccgtagt tcttcaacgt ctcgtgcaag ggaccgacac cgggctcgat ctggcccgac
     1261 ccatggccga tgacctcgtg catgtcgatg tgcagcgccg ccgcgagctc gccatgtcgc
     1321 tcgttacgcg caatctcggc ggcgtcgtac gagaactcgc gatccgcgcc gccgcgcacg
     1381 gcgttgtacg ccgcgacgac gttcgcgagg ctaaccgact tcgagccgtg cgcgcgccga
     1441 atccagtcgg agttcggcag gttgatgccg atcggcgtcg tcggcgcggc gtcgccggcc
     1501 tcgatgacga ccgtgatgac cttgcccgtg atgccggtga cgttcggctt cttgtggcgc
     1561 tcgaggaaag gcatgtggtc ctcgaaccac tgcgcggcgc tcgcgatggc gccgatgcgg
     1621 cgcgtcgcct cgggatcgcg gaacgacacg acgctctcga agctgccgcg catgccgagc
     1681 gggtcgttgt agacctcgat gaagccgtga atcagatcga cgtgtgactc cgtgtcgcgc
     1741 agccacgcga tgttgtagtc gtcccagtcc cgcaagctgc cgctgcgata gaacgcgatc
     1801 agcttcgtca gcgcgtttct ctgcgcgtcg ttctcggcgc agccggccgc tttctcgagc
     1861 cacgcgacca tgcgctcgat ggcttgcgtg tagcggccgc cgatccgctg cacgtcctcg
     1921 acgaccttgc cgtcgacctt gacgagccgc gagttcaatc ccaatgacac cggcgcatcg
     1981 tcgccggcca ctgcctgctg cgcgtagaaa acctcgacct cgcgctgcgt gagcccgctg
     2041 tagaaattga cggccgagct cgtcagcacg tcgcgatcgg cgctcttgct gacgagcttc
     2101 gcgtcgacgg tcgggtcgaa cagcaccggc gtcagctcgg agatgaagtc gtccagcgat
     2161 tgcccgcgcc gcacgggaaa ctcggccggt gtctttcgca cgaagtccgc aaaagccgcc
     2221 ggcgagaaac ccggctcgaa tttgtcgtgc gcgtagtggt gatgcaggcc gttcgcgaac
     2281 cagacgcgct tcaaatacaa ggctagcgcg cgaaacgcgt cggtctcgcg gtcgccggaa
     2341 tagtgccgga cgatgagctc gagcgtgcgt ctgacagcga ggttatagcg gtacttctgg
     2401 tcgtagatga tgtcgcggcc gcaaagcgcc gcttcataca aataatagag aagctccttg
     2461 gtccgcgcgt cgagctgctc gaagcccggc acgcgatagc gcaggatccg cacgtcggcg
     2521 aagcgctcga cctcgacgcg aaatccgttg ccgtcgcccg cgggtcgcac gctgccggga
     2581 ggctctgccc cttgatcggc cgcggtaact gcagccacta tggcggacaa catctacaac
     2641 atcctgaaca cacagggggc caaaccatgc gcaccgtgga gctgcgaaag ccgcaggatc
     2701 atgccatatc gctgcatgtc gccgcagccc tggtattgct cgcgacgctg acgctgtcgc
     2761 ccgcgcgatt cgcagcggca caaggcgccg tcccctgcac ggcgatcgcc aacgacgccg
     2821 agcgcctcgc gtgctacgac cgcgcgctgc gcggcgcgcc gccgccggcc gcttcgcccg
     2881 cgcc
//
LOCUS       DATLEE010000691         4039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3578724, whole genome shotgun sequence.
ACCESSION   DATLEE010000691 DATLEE010000000
VERSION     DATLEE010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3578724"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1955)
                     /locus_tag="VM692_15070"
     CDS             complement(<1..1955)
                     /locus_tag="VM692_15070"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HUQ53546.1"
                     /translation="MKAYSQILLTILALAIGSAVLVVALFIGAYYYVAPGLPQAAELR
                     ETKIRVPLQVYSRDGRLIDEYGEEKRTPVDYEHIPPLLIKAVVAAEDEHFFEHPGIDW
                     RGIVRGAINELRSSGANIGGSTITQQVPRTLDVLKRAGLNSGFDRFVAKFKEWILAYR
                     MEQEFTKEEILELYLNTSFFGQRSYGVATAAQTYFGKTLDELTVSEIAILAGIVQRPT
                     AWNPIASVERAKARRSYVLRRMHETQAIDDEQYQVALEEPIVAQIFGAQRQLEAPYVG
                     EMVRAQMVALFGDTATRAGLKVTTTIDSRLQAASNRGIRDTLMAYDERHGYRGPLARV
                     ELPGAGGEDAAVSAPIDPEALRVLLDEHPSVLDYESAIVLEADELSARVFFSAEGEQS
                     IGFDAVEWAQPFINDDVTGPTPKSVDEVLHPGDVVRFRRTADGGWRLAQIPEAQGAFV
                     SVDPFDGAIVALNGGLDFFLNNFNRATQARRQPGSSFKPFVYSAAFENGYTPASVVLD
                     APPDTGGYQRELERVWRPENFGGKYYGLSRLREALRESMNAVSIRMLQTMGVPAAVAH
                     VKRFGFDDTAVPNDLSLALGSGGVAPVSLAAGYATFANGGYKVTPYFIDRVTTAEGEV
                     LYESKPLICPDCNTPPETPVQLEPPVP"
     regulatory      2010..2107
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2388..3449
                     /locus_tag="VM692_15075"
     CDS             2388..3449
                     /locus_tag="VM692_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HUQ53547.1"
                     /translation="MFATRRKGSLVGLDITTSSVKLIELVASGRGYRVEAYAAEPTPP
                     NAINEKAIVDADAVGEAIRRAHKRAGTKTREAAIAISGDAAITKVIQMPKNLSATDLE
                     GQVELQADQYIPFPMEEVSFDFEVIGDSERDPDMVDVLLVATRTENVEQRQVAVEAAG
                     LQARVVDVEAFAVENACQLLTHQMPDAGADKFIAIVDFGASTTTFSVLKDLKVIYTRD
                     FAFGGQQLTEEIMRTYGLSMEDAGRAKKEGGLPSNYQPEVLDAFIDDMSQQVSRSLQF
                     FLASGSGREQPDQILICGGCANIPGVADVIGSRVGVATQIGDPLGQMKISGRAKSQGV
                     DRDATALLTACGLALRSFD"
     gene            3449..4030
                     /locus_tag="VM692_15080"
     CDS             3449..4030
                     /locus_tag="VM692_15080"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HUQ53548.1"
                     /translation="MPRINLLPWRTELRQRRKKEFLVALAGTLVVAAGVVYLSKLTVQ
                     GWTSSQQGRNEVLRSEIADIDKQIKEIEGLQTQRERLLARMQVITQLQRSRPEIVHLF
                     DELVDAMPEGVNLTEVVQQTNRIELHGVAQSSTRVSALMRNIDNSDFLKEPGLGIVES
                     VASGVDRNSSFQVFAQQVSMVAPDGTAAEGAAQ"
BASE COUNT          778 a         1310 c         1259 g          692 t
ORIGIN      
        1 tccggaaccg gcggctcgag ctgcaccggc gtctccggcg gcgtgttgca gtcagggcag
       61 atcaacggct tgctctcgta cagcacctcg ccctcggccg tcgtcacgcg atcgatgaag
      121 tacggcgtga ccttgtagcc accgttcgca aacgtggcgt agccggccgc gaggctgacc
      181 ggcgccacgc cgccggagcc gagcgccaac gacaagtcgt tcggcacggc cgtgtcgtcg
      241 aagccgaaac gtttgacgtg cgcgacggca gccggcacgc ccatggtctg cagcatgcgg
      301 atcgacacgg cgttcatcga ctcccgcagc gcctcgcgca accgcgacag accgtaatac
      361 ttgccgccga agttctcggg ccgccagacg cgctcgagct cgcgctgata gcccccggtg
      421 tcgggcggcg catcgagcac cacgctcgcc ggcgtgtagc cgttctcgaa cgctgccgag
      481 tacacgaacg gcttgaagga cgagccgggt tgccgacgcg cctgcgtcgc gcggttgaaa
      541 ttgtttaaaa agaaatcgag cccgccgttc aatgcgacga tggcgccgtc gaacggatcg
      601 acggagacga acgcaccctg cgcctcggga atctgcgcga gccgccagcc gccgtcggcc
      661 gtgcggcgga agcgcacgac gtcgcccggg tgcagcacct cgtccaccga cttcggcgtg
      721 ggacccgtca cgtcgtcgtt gatgaacggc tgcgcccact cgacggcgtc gaagccgatc
      781 gactgttcgc cctccgcaga gaagaacacg cgtgcactga gctcgtcggc ctcgagcacg
      841 atcgcgcttt cgtagtcgag caccgaaggg tgctcgtcga gcaacacccg cagcgcctcg
      901 ggatcgatcg gcgccgacac ggccgcatcc tcgccgccgg cccccggcaa ctcgacgcga
      961 gccagcgggc cgcgataccc gtgtcgctcg tcgtaggcca tcaacgtgtc gcgaatccca
     1021 cggttcgaag cagcctgcaa gcggctgtcg atcgtggtcg tcactttgag cccggccctc
     1081 gtcgcggtgt cgccgaacaa cgcaaccatc tgcgcacgga ccatctcgcc aacgtacggc
     1141 gcctcgagct gtcgttgtgc gccgaaaatc tgggcgacga tcggttcctc gagcgcgacc
     1201 tggtactgct cgtcgtcgat cgcttgcgtc tcgtgcatgc ggcgcaatac atagctgcgg
     1261 cgcgccttcg cgcgctctac gctggcgatc ggattccacg ccgtgggccg ctgcacgatg
     1321 ccggcgagga tggcgatctc ggagaccgta agctcgtcca gcgtcttgcc gaagtaggtc
     1381 tgcgccgccg tggcgacgcc ataggaacgc tgcccgaaaa agctcgtatt cagatagagc
     1441 tcgaggatct cttccttcgt gaactcctgc tccattcggt aagccaggat ccattccttg
     1501 aacttcgcga cgaagcgatc gaagccggag ttcaatccgg cgcgcttcag gacgtcgagc
     1561 gtcctcggga cctgctgcgt gatcgtgctg ccgccaatgt tggcgccgct cgagcggagc
     1621 tcgttgatcg cgccgcgaac gatgcctcgc cagtcgatgc cgggatgctc gaagaagtgc
     1681 tcgtcctcgg cagccaccac ggctttgatc agcagcggcg gaatgtgctc atagtcgacc
     1741 ggcgttcgct tctcttcgcc gtactcgtcg atgagtcgcc cgtcgcggct atagacctga
     1801 agcggtaccc gtatcttggt ctcccgcagc tcggcggcct gcggcaagcc cggggccaca
     1861 tagtaataag cgccgatgaa gagggcgacg accagcacgg ccgagccgat cgccaaggcc
     1921 agaattgtca acagaatctg agagtaagct ttcatctgca gagttggatt ctacgccctt
     1981 gccagccctt gggcctttgt gcatagtccg ccgcgtcagg gcaaacccgt cgaaagatgg
     2041 ggacgcaaag cctccggtct acgggcgcga catgggacgt cgcaactagg atagcggggc
     2101 tgccgaagca gttcatcggg gccgtcacaa aattcacccg aagcttcgcc agccactccc
     2161 cgcgttacta ccccgtatgc cgagcgctcg tcccagccga gtagggaatt cagtcacagc
     2221 tggcagattc cgcaacggct accctgcacc ggtccgggta ggctcggccc cggcaatcga
     2281 gaggcccgtc acaacggtgt atatctcggg tgagatatag ttaaattggg cagttgaagg
     2341 cacgtgtagg attctaaccc aacctctcgt gttggaaagg aaaagctgtg tttgccacgc
     2401 gtcggaaagg atccctggtc ggcctcgata tcacgacctc ctcggtgaag ctcatcgagc
     2461 tcgtcgcgag cggtcggggt taccgggtcg aagcctacgc agccgagccg acgccgccga
     2521 acgccatcaa cgaaaaagcc atcgtcgacg ccgacgccgt cggcgaagcc attcgccgcg
     2581 cgcacaaacg cgccggcacg aagacgcgcg aagccgcgat cgcgattagc ggtgatgccg
     2641 cgatcacgaa agtcattcag atgcccaaga acttgagcgc gacggacctc gaagggcagg
     2701 tcgagctgca agcagatcag tacatcccgt tcccgatgga ggaagtcagc ttcgacttcg
     2761 aagtcatcgg cgacagcgag cgcgaccccg acatggtcga cgtgctgctc gtcgcgacgc
     2821 ggaccgaaaa cgtcgagcag cgccaagtcg ccgtcgaagc ggccgggttg caagcccgcg
     2881 tcgtcgacgt cgaagccttc gccgtcgaga acgcctgcca attgctgaca caccaaatgc
     2941 cggacgccgg cgccgacaag ttcatcgcga tcgtggactt cggcgcgagc accactacgt
     3001 tcagcgtgct caaggacctc aaggtcatct acacacgcga cttcgcgttc ggcgggcagc
     3061 agctcaccga ggaaattatg cggacctacg gcctcagcat ggaagacgcc ggccgcgcga
     3121 agaaagaagg agggttgccg agcaactatc agcccgaggt gctcgacgcc ttcatcgacg
     3181 atatgtcgca gcaagttagc cgttcgctgc aattcttcct cgcttccggc agcgggcgcg
     3241 agcagccgga tcaaatcctg atctgcggcg gttgcgccaa catcccgggc gtcgcagacg
     3301 tcatcggcag ccgcgtcggc gtcgcgacgc agatcggcga cccgctgggc cagatgaaga
     3361 tctcgggccg cgccaaatcg cagggtgtag atcgtgatgc gaccgcgctg ctaaccgcgt
     3421 gcgggctcgc actgaggagc ttcgactaat gccaagaatc aacttattgc cctggcgtac
     3481 cgagctgcgc cagcggcgca agaaagaatt cctcgtagcg ctcgccggca cgctggttgt
     3541 ggctgccggc gtcgtctatt tgtcgaagct gacggtccag gggtggacga gttcgcagca
     3601 gggtcgcaac gaggtgctgc gctccgagat cgccgacatc gacaagcaaa tcaaggaaat
     3661 cgagggtctg caaacgcagc gtgagcgctt gctcgcccga atgcaggtca tcacgcagct
     3721 ccagcgctcg cgtcccgaga tcgttcattt gttcgacgag ctcgtggatg cgatgccgga
     3781 aggcgtcaat ctcaccgagg tcgtgcagca aaccaatcga atcgagcttc acggcgtcgc
     3841 gcaatccagc acgcgcgtct cggcgttgat gcgcaatatc gacaactcgg attttctgaa
     3901 ggagccgggc ctcggcatcg tcgagtccgt tgcctctggc gtcgatcgta attcttcgtt
     3961 ccaagtattt gcccaacagg tctcgatggt cgcgcccgac ggcacggcgg ccgagggagc
     4021 agcgcaatga gcatcgtcg
//
LOCUS       DATLEE010000692         2584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11014683, whole genome shotgun sequence.
ACCESSION   DATLEE010000692 DATLEE010000000
VERSION     DATLEE010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11014683"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VM692_15085"
     CDS             complement(<1..696)
                     /locus_tag="VM692_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53549.1"
                     /translation="MTAQSQLEAYLGEFRQRLKALIVARGAAMLAVAALIVTLIAVYF
                     GIRRAFDPQIVISARVILLLLLGGIAVLLVALPLRALTRSRGIKDIERRAPDFNGRLE
                     TYDGIVHGPPERATPFLGLLAEDALTFARRIPVALKIPPLHVRAPAALAAVAFLALVG
                     TAVLGPPNWRYGVRNLWAGWLVSDTLPPQRLAVLPGDGTVRRGGDLHVTAEAEGFEPS
                     RMEVYAQFQEGGQW"
     gene            complement(693..>2584)
                     /locus_tag="VM692_15090"
     CDS             complement(693..>2584)
                     /locus_tag="VM692_15090"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUQ53550.1"
                     /translation="SSLMFLEPGEPRRVLAKKVQYLLLLALRIGVLVLLALAFTEPAL
                     WRTPDPAGDSDGARLHLIVLDGSASMSYDDRWQRARAAASDVLDSMSGDDRAQIVLSG
                     RQFEVLGPATGDVAALRQTLNTAEPGMFRVEYGQLMRSIDGLLRTAELPVVLDLVTDL
                     QTSGLPTRFGELAPRRPAEIVLHDVTDGPAENWTVDSFGASALTGELTASVRSFAPDA
                     VTRTLTLTQNGRSVGEQDVEIAAGGRGQVTFPALELASGANRVEVALSPGDELEGDDR
                     RYLAIKRPEPRKVLLVAPNAEGRDVLFTRSALETLTTLALTADVRATPIGDPPLTSYS
                     FVVVTDLGVLDASQAAAVQDYVQNGGRALLATGPRSAGLTTLPITGQTLRTNPQMGSQ
                     ATVAIGEVDASHPVLRGVDELRAANFSRSVNVEPATGDRVLMSLADGTPLLLEQTVGS
                     GRVLLFTSSLGREWNDLPVQPAFVPLMAGIANELLGGAGFTSEADLGSTLAVRALGLA
                     GGQIFDPRGEAALGLGAGSEDVLLDQVGFYEVVGGGTTELVAVNFDPRESDLTPIEAT
                     TLDRWRGLGVRPGEGATPVAATTADDAVPRSLGPLLMILLLMLVLVESAVGNWHLRIR
                     RGVAA"
BASE COUNT          425 a          906 c          891 g          362 t
ORIGIN      
        1 ccactggccg ccctcctgaa attgcgcgta gacctccata cgcgacggct cgaacccctc
       61 ggcttccgcc gtgacgtgga ggtcgccgcc gcgacggacc gtgccgtcgc cgggcagcac
      121 cgcgaggcgc tgcggcggca aggtgtcgga cacgagccag ccggcccata aattgcgaac
      181 gccatagcgc cagttcggcg gccccaacac ggccgtgccg acgagcgcga ggaaagcgac
      241 ggccgcgagc gcggccggag cgcgcacgtg cagcggcgga atcttcagcg cgacggggat
      301 gcggcgtgcg aaggtcaggg cgtcctcggc cagcaaaccg aggaacggag tcgctctctc
      361 cggcgggccg tggacgatgc cgtcataggt ctcgaggcgg ccgttgaagt cgggggctcg
      421 gcgctcgata tccttgatgc cgcggctgcg tgtgagcgcg cgcaacggca atgcgacgag
      481 aagcacggca atgccgccga gcagcaacag caggatgacg cgcgcgctga tgacgatctg
      541 ggggtcgaag gcgcgccgga tgccgaagta gaccgcgatc agagtgacga tcaacgcagc
      601 gaccgcgagc atagccgcgc cgcgcgcgac gatgagcgcc ttcaggcgct ggcggaattc
      661 gccgaggtag gcttctagct gcgactgggc tgtcatgcgg ccactcctct tcgaatccta
      721 agatgccagt ttcctaccgc ggattccacg agaactagca tcaataacag aatcatcagt
      781 aacggtccca gcgacctcgg aacggcgtcg tcggccgtgg tcgcggcgac cggcgtagcg
      841 ccttcgccgg gtcgcactcc gaggccgcgc cagcgatcca acgtcgtagc ttcgatcggg
      901 gtgagatccg actcacgcgg gtcgaagttc acggccacga gctccgtcgt gccgccgccg
      961 acgacttcgt agaagccgac ctggtcgagc aggacgtcct cgctaccggc gccgaggccc
     1021 agggccgcct cgccgcgcgg atcgaaaatc tgcccgccgg ccagtcccag cgcacggact
     1081 gcgagcgtgc tgccgaggtc cgcttcgctc gtgaagcccg ctccgccgag aagctcgttg
     1141 gcgatgccgg ccatcagcgg cacgaatgcc ggctgcaccg gcagatcatt ccactcgcgg
     1201 cccagcgacg atgtgaagag cagcacgcgg ccgcttccca cggtctgctc cagcaagagc
     1261 ggcgtcccgt cggcgagcga catcagcacg cggtcacccg tagccggttc gacgttcacg
     1321 gagcgcgaga agttcgcggc ccgcagctcg tcgacgccgc gcagcaccgg atgactcgcg
     1381 tcgacttcgc cgatcgccac ggtggcctgc gagcccatct gcgggttggt gcgcagggtc
     1441 tgaccggtga tcggcagcgt cgtcagcccc gctgagcgcg ggcccgtggc cagcagggcc
     1501 cggccgccgt tttgtacata atcttgaacc gcggccgcct gcgaggcgtc gagcacaccg
     1561 agatccgtga ccacgacgaa gctgtagctc gtcagcggcg gatcgccgat cggcgtcgcc
     1621 cgcacgtcgg ccgtaagcgc cagcgtcgtc agcgtctcga gcgcggaccg cgtgaacagc
     1681 acgtcgcggc cttccgcatt cggcgcaacg agcaggacct tgcgtggctc cggccgcttg
     1741 atcgcgaggt agcggcgatc gtcgccctcg agctcgtcgc ctggcgaaag ggccacctcg
     1801 acgcgattgg cgccgctcgc gagctcgagc gcgggaaacg tgacctgccc cctaccgccg
     1861 gccgcgatct cgacgtcctg ctcgccgacg ctgcggccgt tctgcgtcag cgtcagcgtg
     1921 cgcgtcacgg catcgggcgc gaagctgcgc acgctggccg tcagctcgcc cgtcagcgcc
     1981 gaggcgccga agctgtcgac cgtccagttc tcggccggcc cgtcggtcac gtcgtgtaga
     2041 acgatctcgg ccggtcggcg cggcgcgagc tcgccgaagc gcgtcggcaa gccgctcgtt
     2101 tgtaggtcgg tgacgaggtc gagcacgacg ggcagctcag cggtgcgcag cagcccgtcg
     2161 atcgagcgca tgagctgccc gtactcgacg cggaacatgc cgggctcggc ggtgttcagc
     2221 gtctggcgca acgccgcgac gtcgcccgtg gccggcccga gcacctcgaa ctgccgcccc
     2281 gacagcacga tttgcgcgcg gtcgtcaccg ctcatcgagt cgagcacgtc gctcgccgcg
     2341 gcacgcgcgc gctgccagcg atcgtcgtag ctcatcgagg ccgagccgtc gagcacgatc
     2401 aagtgcaggc gcgcgccgtc ggagtcgccg gccggatcgg gcgtgcgcca gagcgcgggc
     2461 tccgtaaacg cgagcgcgag cagcacgagc acgccgatac gcaacgcgag cagcagcaag
     2521 tactgcactt tcttcgcgag cacgcgcctc ggttcgcccg gctcgagaaa catcagcgag
     2581 ctga
//
LOCUS       DATLEE010000693         2247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3587446, whole genome shotgun sequence.
ACCESSION   DATLEE010000693 DATLEE010000000
VERSION     DATLEE010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3587446"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1108
                     /locus_tag="VM692_15095"
     CDS             29..1108
                     /locus_tag="VM692_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HUQ53551.1"
                     /translation="MQLPFVEPPRSLCIIRLSAIGDTCHTVPVVRAIQDAWPATQLTW
                     IIGKTEHSLMKGLEGVELLVIDKGQGLRGYAAVHRALGGRRFDALLHMHASTRANIVS
                     TLVRAPLKIGFDRARARDQQWLFTNRKLAARRERHVMDGLFEFAELIGVKRGAPRWDI
                     PVGADDVAAIAPLFASGKPTLVISPCTGQRFRNYRNWRADWYAEIVDYAAQRYGAQVV
                     LTGGGTAIEQTYGREITATARHKPENVIGKTSLKQLLAILARASVVLCPDSGPAHMAT
                     AVGTPVVGLYATSNRHRTGPYFSQHLVVDKYPQAVAKEFGRPIETLRWGQRVRDPAAM
                     SLITVADVAAKLDAAFAERGVTPVH"
     gene            1131..2039
                     /locus_tag="VM692_15100"
     CDS             1131..2039
                     /locus_tag="VM692_15100"
                     /inference="COORDINATES: protein motif:HMM:NF022457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HUQ53552.1"
                     /translation="MDRYQLQSFSDATPSEIEDAMEKAEVVWFDRCPVELPNEADLSF
                     MRDGLPRELQVKNISYHPESDSIPRFEAAPEVKTRIEQILRTHGQRVEAFLRRSCPDF
                     VPGWTLGTTSFRSIEEQGRKLKPRSSNELVHVDAGAYGATNGARILRFFVNIHPSRDR
                     VWGTKGSFRSIMSRCPELWDAAKGGKPRVRVDKGPLDKLYSGLVDAAGKLYPLMRVID
                     SSPYDRSMRRIHNFMKENPAFRDNPAGYQEIHFPPLSAWMVFTDGISHSVLTGQHALV
                     TTVLIPLSNCRIPELSPYQVLARSAA"
     gene            2050..>2247
                     /locus_tag="VM692_15105"
     CDS             2050..>2247
                     /locus_tag="VM692_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HUQ53553.1"
                     /translation="MAQLHFTQNLVRHTPCPSAELAATTVAELFERYFETWPGVRHYV
                     LDDQGEVRHHVKVLVDGANIKD"
BASE COUNT          394 a          781 c          709 g          363 t
ORIGIN      
        1 ttccgccccg gcgcacgaca ccatcggcat gcagctgcct ttcgttgaac cgccccgttc
       61 gctctgcatc atccgcctct cggccatcgg cgacacctgt cacacggtcc ccgtcgtgcg
      121 cgcgattcag gacgcgtggc ccgccacgca gctgacgtgg attattggta aaaccgagca
      181 ctcattgatg aaggggctcg agggcgtcga gctcctcgtg atcgacaaag gccagggcct
      241 gcgcggctat gccgcggttc accgcgccct tggcggccgt cgcttcgacg cgctgctgca
      301 catgcatgcg tcgacgcgcg ccaacatcgt cagcactctc gtgcgcgcgc cgctgaagat
      361 cggcttcgat cgcgcgcgag cgcgcgatca gcagtggctg ttcacgaacc gcaagctcgc
      421 ggcgcggcgc gaacggcacg tcatggacgg gctgttcgag ttcgccgagc tcattggcgt
      481 caagcgcggc gcgccacgct gggacattcc ggtcggcgcc gacgatgtcg ccgcgattgc
      541 acccttgttc gcgagcggca aaccgacgct cgtgatcagc ccttgcacgg ggcaacgctt
      601 ccgcaactat cgcaactggc gcgcggactg gtacgccgag atcgtcgact acgccgcgca
      661 gcgctacggc gcgcaagtgg tgctcacggg cggcggcacg gccatcgagc aaacgtatgg
      721 ccgcgagatc acggccacgg ctcgtcacaa gcccgaaaac gtcatcggca agacgagctt
      781 gaagcaactg ctcgcgattc tcgcgcgcgc cagcgtggtg ctgtgccccg attccgggcc
      841 ggcgcacatg gcgacggccg tcggcacacc tgtcgtcggc ctctacgcga cctcgaaccg
      901 ccaccggacc gggccctatt tcagccagca cctcgttgtc gacaagtacc cgcaagccgt
      961 ggccaaggag ttcggccggc cgatcgagac gctgcgctgg ggtcagcgcg tccgggaccc
     1021 ggccgcgatg agcctgatta cggtggccga cgtcgccgct aaactagacg ccgcgttcgc
     1081 cgagcgcggc gttacgcccg tgcattagat tcgaagccga gggccgggtc atggatcgct
     1141 accaactgca gagcttcagc gacgctacgc cgagcgagat cgaagacgcg atggaaaagg
     1201 ccgaggtcgt ctggttcgac cgctgccccg tcgagctgcc caacgaggcg gatctcagct
     1261 tcatgcgcga cggcctgccg cgtgagctgc aggtgaagaa catcagctat caccctgaat
     1321 ccgattcgat tccgcggttc gaggccgcgc ccgaggtcaa gacccggatc gagcagatcc
     1381 tgcgcacgca cggccagcgc gtcgaagcgt tcctgcgccg ctcgtgcccc gacttcgtgc
     1441 cgggctggac gctcggcacg acgagcttcc gctcgatcga ggagcagggc cgcaagctca
     1501 agccgcgctc gagcaacgag ctcgtccacg tcgacgccgg cgcctacggc gcgaccaatg
     1561 gcgcgcggat cctgcggttc ttcgtcaaca tccatccgag ccgcgatcgc gtttggggaa
     1621 cgaaaggcag tttccgctcg atcatgagcc ggtgcccgga gctctgggat gccgcgaaag
     1681 gcggcaagcc gcgcgtgcgc gtcgacaagg gaccgctcga caagctgtac agcggcctcg
     1741 tcgacgccgc gggcaagctc tacccgctga tgcgcgtcat cgactcgagc ccgtacgacc
     1801 ggtcgatgcg ccgtatccat aatttcatga aggaaaaccc ggccttccgc gacaatcctg
     1861 cgggttatca agagatccat ttcccgccgc tatcggcctg gatggtgttc accgacggca
     1921 tcagccactc ggtgctgaca ggccagcacg cgctcgtgac gacggtgctg atcccgctct
     1981 cgaactgccg catcccggag ctgtcgccgt accaggtact cgcgcgctcg gcggcttgaa
     2041 ccttcgacga tggcgcagct gcacttcacg caaaacctcg tccggcacac gccgtgcccg
     2101 tcggccgagc tcgcggcaac caccgtcgcc gagctattcg agcgttactt cgagacttgg
     2161 cccggcgtcc gccactacgt gttggacgat cagggcgagg tgcgccacca cgtcaaagtg
     2221 ctcgtggacg gcgccaacat caaagac
//
LOCUS       DATLEE010000694         4040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3592823, whole genome shotgun sequence.
ACCESSION   DATLEE010000694 DATLEE010000000
VERSION     DATLEE010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3592823"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VM692_15110"
     CDS             <1..579
                     /locus_tag="VM692_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HUQ53554.1"
                     /translation="AQPFGIPVMIHVGDTFTPLRELLAMLRSGDVVTHMYAPVNGILD
                     SAGRVLADVREARQRGVRFDIGHGLNEHWRWDVAERALAEGFTPDTISSDLNLPGRTQ
                     QVFDLPNVLSKFLLLGMSLNDVLACVTSRAAGTFRELTSFGTLREGSPADVTVLEMTS
                     GTFEFVDNYKGTRSGSQRLVTRAVVFGGRLVT"
     gene            709..1803
                     /locus_tag="VM692_15115"
     CDS             709..1803
                     /locus_tag="VM692_15115"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HUQ53555.1"
                     /translation="MEHYDQRRRQGGGEDSRRHPQNEERGRDRESWRPDDEHSRRRAG
                     GEERGWLDYDRNQDRPNQRYSDQRRDDQRYGRDGSERQSFSPDWSREGAEPRNSDEPY
                     YSRNRERGGSGDQSRFSEYSNSQSRGWRPEDRSQWEQRSRESIPHEQRELTERSGSGD
                     YYSPDWPYEQGDRGREQRHGEGRYMQRGSEDEQGSHYRGSYSRSSAPFAYPGGRGYMY
                     SESITLHGPYSGRGPKGYKRSDQQIIEEASQRLERDGHIDASEIEVTAEDGLITLRGT
                     VPDRRTKRRAEECVESVYGARDVMNELRCSQESSQQGSPQESQGSQSSQGSRRSQSST
                     RGSQSSSPTSRGTTTGSGSEQPEDKRSPKH"
     gene            complement(1806..2411)
                     /locus_tag="VM692_15120"
     CDS             complement(1806..2411)
                     /locus_tag="VM692_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53556.1"
                     /translation="MSSAVEVEFAASSPADQWDTRTYRAIWNEYGGRIFAAFEAVTCL
                     PFVEPHVAATVANDVSHSGGPEHPMQLRATYARAVKQATLVHELGHRHLWQLTQRLDG
                     VDGHMTLFLVLDRVWAEVWGEDFAEQQIRVESNWEASYDYGKAWQWARGLGKNERARL
                     WDQLLAKNGLAGCGRSSGAPYRAEAAGTAQRRAGPASTLAR"
     gene            2532..3941
                     /locus_tag="VM692_15125"
     CDS             2532..3941
                     /locus_tag="VM692_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53557.1"
                     /translation="MKLANVATALVTALVLSACGPSTREQPFVSVGRGAPVEPKLPYP
                     TTDVASLGDELANYPVVGPFRLAARGKPGEFLELGSAWNGAAPPDIEPLPVDLFTTKD
                     FYQDRALWNDKRYFRCNSGLALEQQHGASRFTVYAIGDDPPRTAAWGYCDRDYPRSAI
                     VSPYPFKTAEEHYEALRAEAKSRGGPTQHTYATVPGEWSGRYARVNVQMAFGTWYGML
                     MNQIPTILSLLTPEYQTRLVQQLFHEGNSNTAQWPGQYCWPEGFMRRYHFAGTQAHQI
                     MVTPSLVQVLSSSSGNFLTNIHVGREFNMEGAVPRLGADVPRWYGETIGFWDGDVLVT
                     WTSNIQGWTSHGLFEFSSKMQTIEIYAPNRDEGGQFVGLNHETVFYDPEALVEPVRIV
                     RDFVKTSGFEEGEPYVFPECIQTIFPVNGLATPVPPGTVLQFEVPDMYGRPWAQIWEK
                     YWEEGMQRPTHDEDIFSFD"
BASE COUNT          698 a         1333 c         1368 g          641 t
ORIGIN      
        1 gcgcagccgt tcggcattcc cgtcatgatc cacgtcggcg atacgttcac gccgctgcgc
       61 gagctgttgg cgatgttgcg gtcgggcgac gtcgtcacgc acatgtacgc gcccgtcaac
      121 ggcatactcg attcggccgg gcgcgtgctc gccgacgtgc gcgaggcgcg gcagcgcggc
      181 gtgcggttcg acatcggcca cggtttgaac gagcactggc gctgggacgt cgccgagcgc
      241 gcgctcgccg aaggcttcac gccggacacg atttcgtcgg atttgaacct cccgggccgc
      301 acgcagcagg tcttcgactt gccgaatgtg ctgtcgaagt tcctgttgct cggcatgagc
      361 ttgaacgacg tgcttgcctg cgtgacgagc cgcgcggccg gcaccttccg cgagctgacc
      421 agcttcggga cgctgcgcga aggctcgcct gccgacgtca cggtgctcga gatgacgagc
      481 ggcacattcg agttcgtcga caactacaaa ggcacgcgct cgggctcgca gcgtctcgtc
      541 acgcgcgctg tcgttttcgg cggtcgcctc gtcacgtagt tccgcgttat tgctcttggc
      601 tctgtgccga cttagtggca cgaaccttgc tgcctgctgg cgctgtcgcc ggtcggtgcc
      661 ggcgcacgcg tgcacgatca tcagagggca caacaaggag gctacgacat ggagcattac
      721 gatcaacgcc gacggcaagg cggcggcgaa gactcgcggc gtcatccgca aaacgaagag
      781 cgcggtcgcg accgcgaatc ctggcggccc gacgacgagc acagtcggcg ccgcgccgga
      841 ggcgaggagc gcgggtggct cgattacgac cgcaatcagg atcggcccaa ccagcgttac
      901 agtgaccaac gccgcgacga tcagcggtat ggtcgagatg gctcggagcg ccagagcttc
      961 tcgccggact ggtcgcgaga gggtgccgag ccgcgcaatt cggacgaacc gtactactcg
     1021 aggaatcgag agcgtggcgg ctcgggcgac caatcgcggt tcagcgagta ctcgaatagt
     1081 cagtcgcgcg gctggcgccc ggaggaccgc tcgcagtggg agcagcgctc gcgcgaatcc
     1141 atcccgcacg agcaacgcga gctcaccgaa cgcagcggct caggcgatta ctacagcccg
     1201 gactggccgt acgagcaggg cgatcgaggt cgcgagcagc gccacggcga gggccgctac
     1261 atgcagcggg gcagcgagga cgagcagggt agccactacc gcggctccta cagccgctcg
     1321 tcggcgccgt tcgcgtatcc gggcggccgc ggctacatgt actccgagtc gatcacgctg
     1381 cacggtccgt attccggccg cgggccgaag ggctacaagc gctcggatca acagatcatc
     1441 gaagaagcga gccagcgcct cgagcgcgac ggccacatcg acgcatcgga gatcgaggtt
     1501 acggccgagg acggcctcat cacgctgcgc ggcacggtcc ccgatcgccg cacgaagcgt
     1561 cgtgccgagg agtgcgtcga atcggtgtac ggcgcgcgcg acgtcatgaa cgagctgcgc
     1621 tgctcgcagg aaagcagtca gcaaggctcg ccgcaggagt cgcaaggatc gcagagctcg
     1681 caaggctcgc gacgatcgca gagcagcacg cgcggctcgc agtcgtcgag cccgacgtcg
     1741 cgcggaacca cgacgggatc cggctcggag caacccgagg acaagcggtc gccgaagcac
     1801 tagacctagc gcgccagggt cgaggcggga ccggcgcggc gttgcgccgt gccggccgct
     1861 tcggctcgat acggtgcgcc gctcgagcgg ccgcagccgg caaggccgtt cttcgcgagc
     1921 agctggtccc agagtcgcgc ccgctcgttt tttccgagcc cgcgcgccca ctgccacgcc
     1981 ttgccgtagt cgtagctcgc ttcccaattc gattcgacgc ggatctgctg ctcggcaaag
     2041 tcctcgcccc agacctcggc ccagacgcgg tcgagcacga gaaataacgt catgtggccg
     2101 tcgacgccgt cgaggcgctg cgtgagttgc cacaaatggc ggtggccgag ctcgtgcacg
     2161 agtgtcgctt gcttgacggc acgcgcgtaa gtcgcacgca attgcatcgg atgctcgggc
     2221 ccgcccgagt ggctgacgtc gttggcgacg gtggccgcga cgtgcggctc gacgaacggc
     2281 aaacaagtga cggcttcgaa tgcggcgaag atgcgcccgc catactcgtt ccagatcgcg
     2341 cgataggtcc gcgtgtccca ctggtcggcc ggcgacgacg cggcgaactc gacttcgacg
     2401 gcgctgctca cgcgcgctcc cagtagtgct gccaccagga caacgccgct cgcgttgcgc
     2461 aggattccgt ttcgtcgccg cattgcgggg catcatgcct cgcaccgcgg cgtcgacgga
     2521 agggggacgc tttgaagctg gccaacgtcg caacggcgct cgtcacggcg ctcgttctct
     2581 cggcttgcgg gccatcgacg cgcgagcagc cgttcgtcag cgtcgggcgc ggcgcgcccg
     2641 tcgagccgaa gctgccgtat ccgacgacgg atgtcgcgtc gctcggcgac gagctcgcga
     2701 actatcccgt cgtaggtccg ttccggctgg cggcgcgcgg caagcccggc gaattcctcg
     2761 agctcggctc ggcatggaac ggcgccgcgc cgcccgacat cgagccgctg cccgtcgacc
     2821 tctttacgac caaggacttc tatcaagatc gcgcgctctg gaacgacaag cgctatttcc
     2881 gctgcaacag cggccttgcg ctcgagcagc agcacggtgc gagccgcttc accgtctacg
     2941 cgatcggcga cgacccgccg cgcacagccg cgtggggtta ctgcgaccgc gactatccgc
     3001 gctcggcgat cgtgagcccg taccccttca agacggccga ggagcactac gaggcgctgc
     3061 gcgccgaagc gaaaagccgc ggcgggccga cgcagcacac gtacgcgacg gtgcccggcg
     3121 agtggtcggg ccgctacgcg cgcgtcaacg tgcagatggc gttcggcact tggtacggca
     3181 tgctgatgaa ccagatcccg acgatcctgt cgctgctgac gccggaatat cagacgcggc
     3241 tcgtgcagca gctctttcac gagggcaact cgaacaccgc gcagtggccg ggtcagtact
     3301 gctggcccga aggcttcatg cggcgctacc acttcgcggg tacgcaagcg caccagatca
     3361 tggtgacgcc gtcgctcgtg caggtgttga gctcgtcgtc gggcaatttc ctgacgaaca
     3421 ttcacgtcgg ccgcgagttc aacatggaag gcgccgtccc gcgcttgggc gccgacgtgc
     3481 cgcgctggta cggcgagacg atcggcttct gggacggcga tgtcctcgtc acatggacgt
     3541 cgaacattca gggctggacg tcgcacggct tgttcgagtt ttcgagcaag atgcagacca
     3601 tcgagatcta cgcgccgaac cgcgatgagg gcgggcaatt cgtcggcctg aatcacgaga
     3661 cggtgttcta cgaccccgaa gcgctcgtcg agcccgtgcg catcgtgcgc gacttcgtga
     3721 agacgagcgg cttcgaggaa ggcgagccgt acgtgttccc cgagtgcatc cagacgatct
     3781 tccccgtcaa cgggctggcg acaccggtcc cgccgggcac ggtgctgcag ttcgaagtac
     3841 cggacatgta cgggcggccg tgggcgcaga tctgggagaa atactgggaa gagggcatgc
     3901 agcgcccgac gcacgacgag gacattttca gcttcgacta gtcctatcga gtccaggccg
     3961 cgctcgcggc atattttgtg catctttccg cgccccgtcg gtgagccgcc taaagggcgg
     4021 cgcatcggac tgggcgttga
//
LOCUS       DATLEE010000695         6728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3597638, whole genome shotgun sequence.
ACCESSION   DATLEE010000695 DATLEE010000000
VERSION     DATLEE010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3597638"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VM692_15130"
     CDS             <1..1059
                     /locus_tag="VM692_15130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HUQ53558.1"
                     /translation="TRFYGERGETVGGDAADDDTDDAPIDEVEQYVRAIERSSGAFQR
                     ELNRRKVRQIRDFFRNETDQPVVPGAVLLFTSEELKFSPIGQYEQMGDLALPRAPFLI
                     IDGQHRLAGLHFYLREPDADRRVEVPCVIFDGKAAEFATEMFVIINSTHTRINKSHLV
                     DLYEKIEWGTDPHKKLAARLVRALYDESDSPLRYHVNMLGGRSQRDMWINQAQLFGEV
                     HRFVTRKDAEAWTTDGRGLSRDRAYGFLRDVLRAAKRVFGDAWGDNKRYMVTRDVTLK
                     ALIRVAGDVAAKSRGLGLSAAASAGATEASLVKRFEPWQSLVAEFRRDGFYERFPARG
                     QVERVEKIRDRLRRDAGL"
     gene            1064..2011
                     /locus_tag="VM692_15135"
     CDS             1064..2011
                     /locus_tag="VM692_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013954167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ53559.1"
                     /translation="MPVIKVTDLAYGRLQSPSLDEAEEFLTAFGLVRSERTPAALYMR
                     GTDSPHHLHVTHRGPSKFAGLGFFAASDEDLAKLARAPGASGVEHVDEPGGGKRVTLT
                     DPLGYRIEVVCGMQSLPPLPAVEHSMNTGGNKLRRAGELLRVERRPSRVKRIAHAVLM
                     TPNAKDKIRWYRDMLGFLPSDEVYEGSKDNIVASFNRCDRGEAFVDHHTFLCFESPTA
                     GLNHLSFEVQSFDDLMAGHEHLHRAGKYRHVWGVGRHLLGSQIYDYWEDPWRRVHEHW
                     TDSDVLNASAQPGLHPIDVGFSAQWGTPPPADFVSHAIP"
     gene            2025..3386
                     /locus_tag="VM692_15140"
     CDS             2025..3386
                     /locus_tag="VM692_15140"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ53560.1"
                     /translation="MIRNDFVSIATLLGCLAATGAQAQAGGPGTPWRGAGAQPCFGID
                     NAAVQCRAPGGVVAIRAGRLFDSRAGQLLADRVVVLRDERIVAVGTPADVAIPAGARV
                     IDLRGATLLPGLIDAHTHMFNNPRPGVTRELATLIAVQNTQADLRAGFTAVRDMSSHD
                     NGYADVDVRNAIDMGLIEGPRAQVSTRGIAWRAPGAAAEDNLLGRALVSNVDEARAAV
                     RDQIEHGADWIKLFPGGAYSFTATGEAQYVTTYPKPVLEALIDETHRLGHKTACHVFG
                     GEGQRNAIAAGCDTIEHAFGLTQEQANEMVRKGLYYDPTFTRYLEPYMDDNDAKSTGG
                     KYSMTKMFAGAVTMAGKTPGLAMMVGSGVDGSTFVHGTQALEFEALVKRGGLTPARAI
                     QAGTIVNARVMGWEDRIGSIEVGKLADLIAVAGDPLADITELQRVAFVMKGGKVIRDE
                     LSQ"
     gene            complement(3500..5248)
                     /locus_tag="VM692_15145"
     CDS             complement(3500..5248)
                     /locus_tag="VM692_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53561.1"
                     /translation="MRLKRNHVVAAALGFLLFGPVTSSALELGDATLTSRLGESLVVE
                     IPYRRAAGEQLTPSCSSLVPPRSSLDALPTYTRIGRVSINSTHIQIFGADRILEPLIG
                     VTVDIRCSTAPRFARSYELFVDLPSRIFTAPGTAARAPAVRPALTAAPAAAAREPVLS
                     PPSSSRDNASPRARGRDGGEIAQGETYRVVRGDTLSGIAARIAGRPGTIRDTADAVFA
                     ANAAAFTRGNRDLLEAGRSITIPILQANAPQPSVAANAVSAVAPRAIENSAAVDASPA
                     IELPAAVEIPRANETPVAVETPAAVETLPPTAAVIDDAAAAPVPATVDTATAPAAPTS
                     VPSSRTAPWLTALLAVGAIILLSAPFALVRRRRDRRPANQADTRARKPAREPVAADVG
                     IKVFEARLPHTPTANTAATRGAVRATASGLTAELPSPTVELDLPIDVTGAVDIDVGVP
                     VVMEERIDWFGQRAAPANDATAGDGSVELNAVTTRMPDFVAAKAPEKALAPQTDRTNE
                     AVNDEHMTLTIAELDLLREDYEAEHTLTQQGSRALQEAVADLEATKARRAVAAETAEL
                     PQATHADTITARVRAK"
     gene            complement(5571..6644)
                     /locus_tag="VM692_15150"
     CDS             complement(5571..6644)
                     /locus_tag="VM692_15150"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53562.1"
                     /translation="MATTTRREAFKWLGGAVAAAVLPSRASAQAPTFPKGAVIRTLFK
                     DYAPEELAGGATLFHEHLSLGPDFSERFRAAAQAVLTAQGAPPTQRPAAPPPPPANDP
                     MRDVDLMTEELRSAQRDGVACIVDAGLEGSGADLDFIRAAAMKSGLPVIKGAGFYVEP
                     FYPKEVATMSEADLVRALVRQADTYPAGVFGEIGSWDEMSAVERKVFRAVGKAHVATT
                     LSIFTHTGIPGKAAIEQLDILEDAGVDPRRVCIGHLGNLNDPNVYVHKTICRRGGFVG
                     FDRQGGNGDANVVPLVLALLDAGFADNVLIAGDASRGYARPVTSFLPKLKEAGADDAT
                     LKRIMVDNSRRFLAFVPKRARKA"
BASE COUNT         1093 a         2199 c         2350 g         1086 t
ORIGIN      
        1 acgcgcttct acggcgagcg cggcgagacg gtcggtggcg acgctgccga cgacgacacc
       61 gacgacgcgc cgatcgacga ggtcgagcaa tacgtgcgcg cgatcgagcg cagctccggc
      121 gcgttccagc gcgagctgaa ccgccgcaag gtgcgccaga tccgcgattt ttttcgcaac
      181 gagacggacc agcccgtggt gccgggcgcg gtgctgttgt tcacgtcaga agagctgaag
      241 ttcagcccga tcggtcagta cgagcagatg ggcgatctcg cgctgccgcg cgcgccgttt
      301 ctgatcatcg acggccagca tcgactcgcc ggcctgcact tctacttgcg cgagcccgac
      361 gccgatcggc gcgtcgaggt gccgtgcgtg atcttcgacg gcaaggccgc ggagttcgcg
      421 accgagatgt tcgtgatcat caattcgacg cacacgcgca tcaacaagag ccatctcgtg
      481 gatctctacg agaagatcga gtggggcacc gacccgcaca agaagctcgc ggcgcggctc
      541 gtgcgcgcgc tgtacgacga gagcgactcg ccgctgcgct atcacgtcaa catgctcggc
      601 ggacgctcgc agcgcgacat gtggatcaac caggcgcagc tgttcggcga ggtgcatcgg
      661 ttcgtcacac gcaaggacgc ggaagcctgg acgacggatg gccgcggcct gtcgcgcgat
      721 cgcgcctacg gcttcctgcg cgacgtgctg cgtgcggcaa agcgcgtgtt cggcgacgcc
      781 tggggcgaca acaagcgcta catggtcacg cgcgacgtga cgctgaaggc gctgatccgc
      841 gttgccggcg acgtcgcggc gaaatcgcgc gggctcggct tgtcggcggc cgcgagcgcc
      901 ggcgctacgg aggcgtcgct cgtcaagcgc ttcgagccgt ggcagtcact cgtcgccgag
      961 ttccgccgcg atggtttcta cgagcgcttt ccggcgcgcg gtcaagtcga gcgcgtcgag
     1021 aagattcgcg accgcttaag gcgcgacgcc ggtttgtgag ctcgtgcccg tcatcaaagt
     1081 cacggatctc gcgtacggcc ggttgcaatc gccgagtctc gacgaggccg aggagttcct
     1141 gacggctttt gggctcgtgc gctcggagcg cacgcccgcc gcgctctaca tgcgcggcac
     1201 cgattcgccg catcacttgc acgtcacgca tcgcgggccg tcgaagttcg ccgggctcgg
     1261 gttcttcgcc gcgagcgacg aagaccttgc aaagctcgcg cgtgcgccgg gtgccagcgg
     1321 cgtcgagcac gtcgacgagc cgggcggcgg caagcgcgtg actttgacgg atccgctcgg
     1381 ctaccgaatc gaagtcgtct gcggaatgca gtcgctgccg ccgctgccgg cggtcgagca
     1441 ctccatgaac acgggcggga acaagctgcg ccgagccggc gagctattgc gcgtcgaacg
     1501 gcgcccgtca cgcgtcaagc ggattgcgca tgcggtgctc atgacgccga acgcgaagga
     1561 caagattcgc tggtaccgcg acatgctggg gttcctgccg tccgacgaag tctacgaagg
     1621 ctcgaaggac aacatcgtcg cgtccttcaa ccgctgcgac cgcggcgagg cgttcgtgga
     1681 ccatcacacg tttctgtgct tcgaatcgcc gacggcgggg ttgaatcact tgtcgttcga
     1741 ggtccagagc ttcgacgatc tcatggcggg tcacgagcac ttgcatcgag ccggcaaata
     1801 ccgccacgtc tggggcgtgg gccgccatct gctcggcagc cagatctatg actactggga
     1861 agacccctgg cggcgcgtcc acgagcactg gaccgattcc gacgtgctga atgccagcgc
     1921 gcaacccggc ctgcatccga tcgacgtcgg gttcagcgcg caatggggca cgccgccgcc
     1981 ggcggacttt gtgagtcacg cgattccttg agggagaacg cccgatgata cgcaacgact
     2041 tcgtctcgat cgcaacgctg ctcggctgct tggcggcgac gggcgcgcag gcgcaagccg
     2101 gcggcccggg cactccgtgg cgcggggcgg gagcgcagcc gtgcttcggc atcgataacg
     2161 cggccgtgca gtgtcgcgcg ccgggaggcg tcgtcgcgat tcgcgccggc cgcctgttcg
     2221 acagccgcgc aggccagcta ctcgcggacc gcgtcgtcgt gctgcgcgac gagcgcatcg
     2281 ttgcggtcgg gacgccggcg gacgtcgcga ttcccgccgg tgcgcgcgtg atcgatttgc
     2341 gcggcgccac gctgttgccg gggctcatcg acgcgcacac gcacatgttc aacaacccgc
     2401 ggcccggtgt gacgcgcgag ctcgcgacgt tgatcgccgt gcagaacacg caagcggatc
     2461 tgcgcgccgg cttcacggcc gtgcgcgaca tgagcagcca cgacaacggt tacgccgacg
     2521 tcgacgtgcg caatgcgatc gacatgggct tgatcgaagg tccgcgcgcg caagtatcga
     2581 cgcgcggcat cgcctggcga gcgccgggcg cggcggccga ggacaacttg ctcgggcgcg
     2641 cgctggtaag caacgtcgac gaagcgcgcg cggccgttcg cgatcaaatc gagcacggtg
     2701 cggattggat caagctattt cccggcggcg cgtattcgtt caccgcgacg ggcgaggcgc
     2761 agtacgtcac gacttatccc aagcctgtgc tcgaagcgct gatcgacgag acgcaccgcc
     2821 tgggccacaa gaccgcgtgc cacgtgttcg gcggcgaagg gcaacgcaat gcgatcgcag
     2881 ccggctgcga cacgatcgag cacgcgttcg gtctcacgca agagcaagcc aacgagatgg
     2941 tgcgcaaggg tctgtactac gacccgacgt tcacgcgcta tctcgaaccg tacatggacg
     3001 acaacgacgc gaagagcacc ggcggcaagt acagcatgac gaagatgttc gcgggcgccg
     3061 tcacgatggc cggcaagacg cccggcctgg caatgatggt cggcagcggc gtcgacggtt
     3121 cgacgttcgt gcacggcaca caagcactcg agttcgaagc gctcgtgaag cgcggcgggc
     3181 tcacgcccgc gcgcgccatt caggccggca cgatcgtcaa cgcgcgagtc atgggatggg
     3241 aggaccgcat cgggtcgatc gaagtcggca agctcgccga cctgatcgcc gtggccggcg
     3301 acccgctcgc cgacatcacg gagctgcagc gcgtcgcgtt cgtcatgaag ggcggcaagg
     3361 tcatccgcga cgagctttcg cagtagccct gcggcgaggc gctcagctcc agagcgcgcc
     3421 gagctcgagc tcgattgcgt cgaagggcgc cgtgcggact aggacgtacg cgcagttcgc
     3481 acgggtctct atcggagcgc tatttcgcgc gaacccgtgc cgtgatcgtg tcggcgtgcg
     3541 ttgcctgcgg caactccgcg gtttcggctg ccacagcgcg tctcgctttc gtcgcttcga
     3601 gatcggcgac ggcctcctgt agcgcccggc ttccctgctg cgtcaacgtg tgctccgcct
     3661 cgtaatcctc gcgcagcagg tcgagctcgg cgatcgtcaa cgtcatgtgc tcgtcgttca
     3721 cggcttcgtt ggtgcggtcc gtttgcggcg caagcgcttt ctcgggcgct ttggccgcga
     3781 cgaagtccgg catgcgagtc gtgacggcgt tcagctcgac ggacccgtca ccggccgttg
     3841 cgtcgttcgc cggagcggcg cgctgaccga accaatcgat gcgctcttcc atcacgaccg
     3901 gtactccgac atcgatatcg acggcgccgg tcacatcgat cggcaggtcg agctcgacgg
     3961 tcggggacgg caactcggca gtcaagccgc ttgcagttgc gcgaaccgcg ccgcgcgtcg
     4021 cggccgtgtt cgctgtcggc gtgtgcggca atcgagcttc gaagactttg atgccgacgt
     4081 ccgctgcgac cggctcgcga gccggttttc gggcgcgggt gtcggcttga ttcgccggac
     4141 gccgatcgcg tcgccggcgt acgagcgcaa acggcgcgga caatagaatg atggcgccga
     4201 cggccagcag cgcggtcaac cacggagccg tacgcgagct cggcaccgaa gtgggcgctg
     4261 cgggcgcggt tgccgtgtcg acggttgcag ggaccggcgc ggcggccgcg tcgtcgatga
     4321 cggcggccgt cgggggcagc gtctcgactg cagcgggggt ctcgactgca acgggcgtct
     4381 cgtttgcacg cgggatctcg acggctgcag gtagctcgat cgccggcgaa gcatccaccg
     4441 cggcggagtt ctcgatcgca cgcggggcga cggcgctgac agcattcgcc gcgaccgacg
     4501 gttgcggcgc attcgcctgc aggatgggaa tcgtgatcga gcggccagct tcgagcagat
     4561 cacgattgcc gcgcgtgaac gcagcagcgt tggccgcgaa cactgcatcc gcggtgtcgc
     4621 gaatcgtgcc cggtcttccg gcaattcgcg ccgcgatgcc ggacagcgtg tcgccgcgga
     4681 cgacgcggta cgtctcgcct tgcgcgatct cgccgccgtc acggccgcgc gcgcgtggcg
     4741 aggcgttgtc gcgcgacgac gacggcgggc tcagcaccgg ttcgcgtgct gcagccgcgg
     4801 gcgctgcggt cagcgcgggc ctgacggcag gcgcacgggc agcggtaccc ggtgcggtga
     4861 agattcgaga cggcagatcg acgaacagct cgtaactgcg cgcgaaacgc ggcgccgtgg
     4921 agcagcgaat gtcgacggtg actccgatca gcggctcgag gattcgatcc gcgccgaaga
     4981 tctgaatgtg cgtggagttg atcgatacgc ggccgatgcg cgtgtacgtc ggcagcgcgt
     5041 ccaagctgct cctgggcggc acgagagagc tgcatgaagg cgtgagctgc tcgccggccg
     5101 cgcggcgata gggaatctcg acgacgagcg attcaccgag acggctggtc agcgtcgcgt
     5161 cgccgagctc gagcgcagag ctcgtcacgg gaccgaatag caagaaccca agtgctgccg
     5221 caacgacgtg gtttcgcttc agcctcaagg ctcgcctggg ctcgcgtgcg agccgcgggg
     5281 cgatcagacg gggggaggca ttggtcctag ggcgcgttgt cgtgtgtggc atcgcttgtt
     5341 tgccggcgga attctaactt ccgactattt gcgccagctt acgcgatggc cgaaacggtt
     5401 accgcgacgg ggttcaagtt tgcgaccaaa cgttggagcc gccgcgactt cgccgcagcc
     5461 gcgcgctgcc gcagttcaac ttaacgctga ggagctgcgc gaatcgcagt tgtaaagaat
     5521 atcggcgtgt ggctgtgggc cgttccagcc gatcccgagc gtgccggtgc tcaggctttg
     5581 cgcgcgcgct tcggcacgaa cgcgaggaat cgccgcgagt tgtcgaccat gatgcgcttc
     5641 agcgtcgcgt cgtcggcgcc ggcttccttg agcttcggca agaacgacgt gacgggccgt
     5701 gcatagccgc gcgacgcgtc gcccgcgatc aggacgttgt cggcgaaacc ggcgtcgagc
     5761 aatgcgagca cgagcggcac gacgttcgcg tcgccgttcc ccccttggcg gtcgaagccg
     5821 acgaacccgc cgcggcggca gatcgttttg tgcacataga cattggggtc gttcaagttg
     5881 ccgaggtgcc cgatgcaaac gcgccgggga tcgacgccgg cgtcctcgag aatgtcgagc
     5941 tgctcgatcg ccgccttacc ggggatgccc gtgtgcgtga agatcgagag agtggtcgcg
     6001 acgtgcgcct tgccgacggc gcggaacacc ttgcgctcga cggcgctcat ctcgtcccac
     6061 gagccgatct cgccgaacac gccggccggg taggtgtcgg cctggcgcac gagcgcgcgc
     6121 acgaggtcag cctcgctcat cgtcgcaacc tccttcggat agaacggctc gacgtagaag
     6181 cccgcgcctt tgatgaccgg caaaccggac ttcatcgccg ccgcgcggat gaagtcgaga
     6241 tccgcgcccg agccctcgag gcccgcgtcg acgatgcacg cgacgccgtc gcgctgcgcg
     6301 ctcctgagct cctccgtcat gaggtcgacg tctcgcatcg gatcgttggc cggtggcggc
     6361 ggcggagcgg cgggacgctg cgtcggcggt gcgccctgcg ccgtgagcac ggcttgcgct
     6421 gctgcgcgga agcgctcgct gaagtccggc cccaacgaca gatgctcgtg aaacagcgtg
     6481 gcaccgcccg ccagctcctc gggggcgtag tccttgaaca acgtccggat gacggcgcct
     6541 ttcgggaacg tcggcgcttg cgccgacgct cgtgacggca gcaccgccgc ggcgaccgcg
     6601 ccgcccagcc acttgaacgc ctcgcgccgt gtagtcgtcg ccatgcggat cccccgtgtg
     6661 agtcggcgaa gaggggccag catatcgctc gcgaacgaga cggggccaga ccggctggtg
     6721 cgatgacc
//
LOCUS       DATLEE010000696         2459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7307025, whole genome shotgun sequence.
ACCESSION   DATLEE010000696 DATLEE010000000
VERSION     DATLEE010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7307025"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1467)
                     /locus_tag="VM692_15155"
     CDS             complement(100..1467)
                     /locus_tag="VM692_15155"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HUQ53563.1"
                     /translation="MNTAVARKRSLSPSPASTGPAATVEVTTPSEIHQLLMDVKRYPS
                     PVRPVGSGSSTTRCVTANGGTQVDLSRMNRVLKIERDRVTVQPGITLTELADVLSDEG
                     LELVGGFDLANRSVGGAVSAAGLEAAMAGDVGQFASHVVQLKVLSPNGKKFVVSEKTK
                     SLLALMRLSYGLLGIVYEVTLRVRPVQGFAVQTAKVSFKDFGKLGQKLLGATAGVKLY
                     LLPFRDRIYLELRRPAAEGDPGRRFAWRLKDWAVYSALPEAARSLGIAVPIRQIRYPL
                     IDSLSEVAQRLVNNALVRSGSNSVEQSGRYRMLGSKGRFTYTTWAFPAAEFASTALAY
                     KLFCKEYYARTGFRCDMPTVGFRLNRDRSALLSPSFDSPMFTISPLSTQVDGWDDFVL
                     DFADFAAKNHGVPFFNQTRNASPEVVNQRFGTRLAFFNKVRRELDPHDRLVNQFFQSY
                     LPQST"
     regulatory      complement(1547..1637)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1728..>2459)
                     /locus_tag="VM692_15160"
     CDS             complement(1728..>2459)
                     /locus_tag="VM692_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53564.1"
                     /translation="SSYPPAKISTGYARRAGNDTNVIWIYGNAPVFPWDELSSVPTEE
                     GFRAMARDSVERYRSAVIDMRRLVDWATMQPDIDASRIAFVGFSMSALVTATLLANEP
                     RVSAGVLMMGAARFSDIFSMCRNRAGDVREHAVGAFGWTQDQYHDFFEELFSPADPVR
                     FEGRYDPDKILMIDAMFDDCMPESSRAALWEITGHPQRVTFLYQHRSAFYSLTPIGLN
                     VSRRKIYRFLDGVLGRDRRDTATAD"
BASE COUNT          436 a          772 c          823 g          428 t
ORIGIN      
        1 tccggcgcgg gcgcctctct gcacccgcac cgaacctgga cgtgcaatgc cgcgcggggt
       61 acgactgcgt gcaagacctc tttcttggtt cgcgttcggc tacgtcgact ggggcaggta
      121 gctctggaag aactggttga cgagacggtc gtgcgggtcg agctcgcggc gcactttgtt
      181 gaagaaggcg agccgcgtgc cgaagcgttg gttcacgacc tcgggcgaag cgttgcgcgt
      241 ttgattgaag aacggtaccc cgtgattctt ggccgcgaag tcggcgaagt cgagcacgaa
      301 atcgtcccag ccgtcgacct gcgtggacag cgggctgatc gtgaacatcg ggctgtcgaa
      361 cgacggcgag agtagcgcgc tgcgatcgcg attcaggcga aacccgaccg tcggcatgtc
      421 gcagcggaag cccgtgcgtg cgtagtactc cttgcagaac agtttgtacg ccagcgccgt
      481 gctcgcgaac tcggcggcgg ggaacgccca agtcgtgtac gtgaatcgcc ccttgctgcc
      541 gagcatgcgg tagcgaccgc tctgctcgac ggagttgctg ccgctacgca cgagcgcgtt
      601 gttgacgaga cgttgcgcga cctcgctcaa gctgtcgatc agcggatagc gaatctgacg
      661 aatcggcacg gctatgccga gcgagcgcgc ggcttcgggc agggccgagt agacggccca
      721 gtccttgagc cgccacgcga acctgcggcc cgggtcgccc tcggccgcgg ggcgccgcag
      781 ctcgaggtag atgcggtcgc gaaacggcag cagatagagc ttcactccgg ccgtggcgcc
      841 gagcagcttc tggccgagct tgccgaagtc cttgaacgat accttggccg tttgcacggc
      901 aaagccttgc acgggccgca cgcgcagagt gacctcgtag acgatgccaa gcaggccgta
      961 gctcaaccgc atgagcgcga gcaggctctt ggtcttctca ctgacgacga acttcttgcc
     1021 gttcgggctc aacaccttga gctgcacgac gtgcgaagcg aactggccga cgtcgccggc
     1081 catcgcggcc tccaggccgg ccgccgagac ggcgccgccg acgctgcgat tcgcgagatc
     1141 gaagccgccg acgagctcga gcccttcgtc gctgagcacg tccgcgagct ccgtcaacgt
     1201 tatgccgggc tgcacggtca cgcgatcacg ctcgattttc agcacgcgat tcattcgcga
     1261 cagatcgact tgcgtgccgc cgttcgccgt gacgcaacgc gtcgtggagc tgccggagcc
     1321 gacggggcgc acggggctcg gatagcgctt cacgtccatc aagagctgat ggatctcgct
     1381 cggagtcgtc acttcgacgg tggcggccgg gccggtagag gcaggcgatg ggcttaggct
     1441 gcgcttgcgc gccacggcgg tattcatgcg ctgacggact ccctaatttc cgtgtcgccg
     1501 gtcgagacga cgaatgggag tgccgcgcgc gcaaagcaag cgattggccg gcaaccccgc
     1561 tgtcgtagga cctagtaagg tccgttagac cggaagcttt gtgtccccgg ctttcgccgg
     1621 gtttactctt gatcagctga agccgactat cgccgagtgc ggtgtctcat gcaagcgact
     1681 cgcgcgcacg aagcgaagga tccgcgcgag tcggagcggg agttatgtca atccgctgtc
     1741 gccgtgtcgc gacggtcgcg tccgaggacg ccgtcgagga agcgataaat cttgcggcgc
     1801 gaaacgttga ggccgatcgg cgtcaacgaa tagaacgcgc tgcggtgctg atagagaaac
     1861 gtcactcgct gcggatgccc cgtgatttcc catagcgcgg cgcgcgacga ctccggcatg
     1921 cagtcgtcga acatcgcgtc gatcatcaag attttgtcgg gatcgtagcg gccctcgaag
     1981 cgcacgggat cggccggaga gaacagttcc tcgaaaaaat cgtggtactg gtcttgtgtc
     2041 cagccgaacg cgcccaccgc gtgctcgcgc acgtcgccgg cacgattgcg gcacatcgag
     2101 aagatgtccg agaagcgcgc cgcccccatc atcagcacgc cggccgatac acgcggctcg
     2161 ttcgcaagca gcgtggccgt cacgagcgcg ctcatgctga agccgacgaa cgcgatgcgc
     2221 gatgcatcga tgtcgggttg catcgtcgcc caatcgacga ggcgccgcat gtcgatgacg
     2281 gccgagcggt aacgctcgac gctgtcgcgc gccattgcgc ggaagccctc ctcggtcggc
     2341 acggagctca gctcgtccca cgggaacacg ggtgcgttgc cgtagatcca gatcacgttc
     2401 gtgtcgttac cggcacggcg cgcatagccc gtggagatct tcgcgggcgg gtagctgct
//
LOCUS       DATLEE010000697         5066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11031297, whole genome shotgun sequence.
ACCESSION   DATLEE010000697 DATLEE010000000
VERSION     DATLEE010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11031297"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..914)
                     /locus_tag="VM692_15165"
     CDS             complement(63..914)
                     /locus_tag="VM692_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53565.1"
                     /translation="MPRILGPCSLLTAPVAALLLCAPPFAVDAQWRNAPQDKGPRNAS
                     GEIDLEAPPPRHANGRVDLQGVWMPDDNRYIRDLALDLGDDKVPYQPWARRLFDERRD
                     GSHSGEDPDAHCLPQGVPKIDFVSYPWKLVETPDSVAILYETFTFWRQIFTDGRTVDS
                     TVKPAWMGYSAGRWEGDTLVVETTGFNGKIWLDQLGRPTTDRLRVTERFTRTHFGHMR
                     IDVTIDDAGAYTAPWSASQVVHLRPGWEPLEFICGENNKDVENLPGDGGPVLPNIDAA
                     RRKGYLD"
     gene            1001..1390
                     /locus_tag="VM692_15170"
     CDS             1001..1390
                     /locus_tag="VM692_15170"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53566.1"
                     /translation="MHRCVLLSSLAALLIGGGPAGAHHSFSAIFDANKTVTLTGTVTR
                     LEWMNPHTWIYLDVTKDDGSVEEWSFEMGSPNRLMRYGWNQDSLPKGQTVTIAGSQAR
                     DGSRKAAVDTVTLPDGKRLFGAQDTSN"
     gene            complement(1391..2374)
                     /locus_tag="VM692_15175"
     CDS             complement(1391..2374)
                     /locus_tag="VM692_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53567.1"
                     /translation="MTPLRSTVALRLRVAVWLAASLGATHFAAAHELELASIEQARAV
                     LGTSDDFVAHMSPFDRAARVESGDAVSVDEYLAFARAAGREWSNDERARLAAAFAAIE
                     PKLEELLPELDRPILLVKTSGQEEGGAGYTRGNAVMLPQAHEEELQLQHLLAHEIFHV
                     VSRNNPDLKRALYRGIGFEECGELVLPPALAARKMTNPDAPVNEHCIEIAVDGEKAWG
                     MPILLSREERFDPDAGRAFFEYLTLSMLLVDRGSGATRPLERNGAPVLVPFNRVQGLG
                     DKIGHNTNYVIHAEEILASNFELLVMGAPDAPSPEVLERIRTELTSAVARR"
     gene            complement(2464..2835)
                     /locus_tag="VM692_15180"
     CDS             complement(2464..2835)
                     /locus_tag="VM692_15180"
                     /inference="COORDINATES: protein motif:HMM:NF016211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HUQ53568.1"
                     /translation="MTNETPMFDSLSTKLIACAIIVACLLLGLAGLILPLIPGLLFIA
                     IAAFVAAKLSPRFAAFVRENDTLRGYLDQADRIAGVPLAQKIQVSGLLLLKMLIDGVA
                     LLVAAVMKLVKAAEGLSARQP"
     gene            complement(2846..3124)
                     /locus_tag="VM692_15185"
     CDS             complement(2846..3124)
                     /locus_tag="VM692_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53569.1"
                     /translation="MNVLGTLITVLAVVVGAVLLIPILGVAFGLVVALGGVLLWLLPI
                     VLIAASDKVGTAEKLLWILAIVCLSWFAWIFYFFFAPVFDRPQRRSYY"
     gene            complement(3308..4168)
                     /locus_tag="VM692_15190"
     CDS             complement(3308..4168)
                     /locus_tag="VM692_15190"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ53570.1"
                     /translation="MAPTRRDLIKTGAAVAAAAAASRTLAQTAGGSSGGTFYEKGAVR
                     IRYLDTGGSGFPLLIIPGGGLNSTISGLINSHPFNPIDAFKNEFRCITCDLRNANGGE
                     STGPLEVERPWDAFTDDHVGIMDHLGIDKFMVLGFCIGGPFIWNLLERAPNRVVAAAT
                     AQPSGYRPEQPTLFYDNNMMGWGPEIVKRRPEITMQTVDRFLTRMYKTNPDFVFTVTR
                     DFVKQCQTPVLVLPDDIPAHPHAVAMEVVMLAPQSEVSIYPWKEPPERIPIAVRQIRS
                     FLRAHRPVKT"
     gene            4315..>5066
                     /locus_tag="VM692_15195"
     CDS             4315..>5066
                     /locus_tag="VM692_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HUQ53571.1"
                     /translation="MRLSSSFAAGCALLLAATTGTAAQRVLFIGNSFTYGAGSAVRFY
                     RNDSVTDLNDEGVGGVPALFKSFADEAGLDYDVALETRGGTGLDFHLAEKRDLIVRRG
                     WDVVVAHGYSTLDADAPRDPQKLIATSRELAAALRSASPEVELFLMATWSRADQVYPA
                     EGAWAGRPIETMATDIRAAYDAAAAAAKAKVIPVGTAWNRAIRDGVADPNPYDGVEAG
                     KLDLWTYDHYHASTYGYYLEALVIFGSVTGRDP"
BASE COUNT          928 a         1673 c         1657 g          808 t
ORIGIN      
        1 ctcgacggtt gttgcagtcg aaattaatgc ggcgcacgac gcgggaacga cgccgtcgta
       61 cgttagtcga gatagccttt gcgccgcgca gcgtcgatgt tcggcagcac gggcccgccg
      121 tcgcccggca gattctcgac gtccttgttg ttctcgccgc aaatgaactc gagcggctcc
      181 cagccgggcc gcagatgcac gacttgcgac gccgaccaag gcgccgtgta ggcgccggcg
      241 tcgtcgatcg tcacgtcgat gcgcatgtgc ccgaaatgcg tgcgcgtgaa gcgctccgtg
      301 acccgcaatc gatccgtcgt cgggcggcca agctggtcga gccaaatctt gccgttgaag
      361 cccgtcgtct cgacgacgag cgtgtcgccc tcccatcgtc cagccgagta gcccatccac
      421 gccggcttca cggtcgaatc gacggtgcgc ccgtccgtaa aaatttgccg ccagaacgtg
      481 aacgtctcgt agaggatcgc cacggaatcc ggggtctcga cgagcttcca cgggtacgac
      541 acgaaatcga tcttcggcac accctgcggc aggcagtgcg catcgggatc ctcgccggag
      601 tgcgagccgt ccctgcgctc gtcgaacagc cgtcgagccc acggttgata cggcaccttg
      661 tcgtcgccga ggtcgagcgc gagatcgcgg atgtagcgat tgtcgtcggg catccagacg
      721 ccctggaggt cgacgcgtcc gttcgcgtgg cgcggcggcg gcgcttcgag gtcgatctcg
      781 ccgctcgcgt tgcgcggacc tttgtcttgc ggggcgtttc gccactgggc gtcgacggcg
      841 aacggcggcg cgcataggag cagcgccgcg acgggcgccg tcagcagaga acaagggccg
      901 agtatgcgcg gcatcgaggc aaaacctacg tcggttccac gaagcagtca acgcggacta
      961 gaattgcgac gagtacccga gtcatgcgcg ggagagaacc atgcaccgat gcgttctatt
     1021 gtcgagcctc gccgctctgc tcatcggcgg cggacccgcc ggggcgcacc actcgttctc
     1081 cgcaatcttc gatgcgaaca agaccgtgac cttgacgggc accgtcacgc gcctcgagtg
     1141 gatgaacccg cacacctgga tctatctcga cgtaacaaag gacgatggca gcgtcgagga
     1201 gtggtcgttc gagatgggca gcccgaaccg gctgatgcgc tatggctgga accaggactc
     1261 gctgccaaag ggccagaccg tgacgatcgc aggctcgcaa gcgcgcgacg gcagccgcaa
     1321 ggcggccgtc gatacggtca cgctgcccga cggcaagcgg ctattcggcg cgcaggacac
     1381 ttcgaactga tcagcggcgc gcgacggcgc tcgtgagctc cgtgcggatg cgctcgagca
     1441 cctcgggcga cggcgcgtcc ggcgcgccca tcaccaacag ctcgaagttc gacgcgagga
     1501 tctcctcggc gtggatcacg tagttcgtgt tatggccgat cttgtcacct aatccttgca
     1561 cgcgattgaa cggtacgagc acgggcgcgc cgttgcgctc gagcggacgc gtggcgccgg
     1621 agccgcgatc gacgagcagc atcgacaacg tcagatactc gaaaaacgcg cggcccgcgt
     1681 caggatcgaa gcgctcctcg cgcgacaaca gaatcggcat gccccaggcc ttctctccgt
     1741 cgacggcgat ctcgatgcaa tgctcgttca cgggcgcgtc gggattcgtc atcttgcggg
     1801 cggcgagagc cggcggcagc acgagctcgc cgcactcctc gaagccgatc ccacgatata
     1861 gcgcgcgctt caagtccgga ttgttgcgcg atacgacgtg gaagatctcg tgcgccagca
     1921 agtgctgcag ctgaagctcc tcttcatgcg cctgcggcag catgacggcg ttgccgcgcg
     1981 tgtagccggc gccgccctct tcttggccgc tcgtcttcac gagcaggatc gggcggtcga
     2041 gctccggcaa gagctcctcg agcttcggct cgatggccgc aaacgctgcc gcgaggcgcg
     2101 cacgctcgtc gttcgaccac tcgcgtcccg cggcgcgcgc gaacgcgagg tattcgtcga
     2161 cggagactgc gtcaccggat tcgacgcgcg ccgcgcgatc gaacgggctc atgtgcgcga
     2221 cgaaatcgtc gctcgtgccg agcacggcgc gtgcctgctc gatcgaagcg agctcgagct
     2281 cgtgcgcggc agcgaagtga gtcgcgccga gcgaggccgc gagccaaaca gcgacacgga
     2341 gacgcagcgc gacggtcgat cgaaggggag tcatgagaac gcaccgatca gcgcgacagc
     2401 gcgcgctgcc agtctagcgc ggatcgtcgg acccgcggac gatccgcacc aacttaggcg
     2461 ccctcagggc tggcgagcgc tcaagccctc cgcggccttc acgagcttca tgacggcggc
     2521 gacgagcaac gcaacgccgt cgatcagcat cttcagcagc agcaagccgc tgacttgaat
     2581 tttttgcgca agcggcacgc cggcgatgcg atccgcttga tcgagatagc cgcgcagcgt
     2641 gtcgttctcg cgcacgaaag ctgcgaagcg cggcgacagt ttcgcggcga cgaatgcggc
     2701 gatcgcgatg aacagcaagc ccggaatcaa cggcaggatc agtccggcca agcccagcag
     2761 caagcacgcc acgatgattg cgcaggcgat gagcttcgtc gaaaggctgt caaacatcgg
     2821 cgtctcgttg gtcattgctg caccctcagt agtagctacg gcgctgcggc cggtcgaaga
     2881 ccggcgcaaa gaagaagtag aaaatccagg cgaaccacga caggcagacg atcgccaaaa
     2941 tccacaggag cttttcggcg gtaccgacct tgtcggaggc ggcaatcagc acgatcggca
     3001 ggagccacag cagcacgccg cccaacgcca caacgaggcc gaacgccacg ccgagaatcg
     3061 gaatcagtag aaccgcgcca accacgacgg ccagaacggt gatcaaggtc ccgagcacgt
     3121 tcacgatgga ttcccctccg tagcgagctc acgcgcccgc gagcaggacc attgccaaac
     3181 tcgtgccaat agccggttgc agcgatttca agaagttagc gccgcgggca tggcgggcca
     3241 cgccgcctcg tgatcggcgc tcgccaacaa ttggcgaaat agaccaacgc gtcgtgcgcg
     3301 gcctccttca ggtcttcacg ggccggtgag cgcgcaagaa cgagcggatc tgccgcacgg
     3361 cgatcggaat tcgctcgggc ggctccttcc acgggtagat gctgacctcg gactgcggcg
     3421 ccagcatcac gacttccatc gcgacggcgt gcgggtgcgc cgggatgtcg tcgggcagca
     3481 cgagcaccgg cgtctgacac tgcttgacga agtcgcgcgt cacggtgaac acgaagtcgg
     3541 gattcgtctt gtacatgcgc gtgaggaacc ggtcgacggt ctgcatcgtg atctcgggcc
     3601 ggcgcttcac gatctccggc ccccaaccca tcatgttgtt gtcgtagaac agcgtcggct
     3661 gctcgggacg gtagccgctc ggctgcgcgg tcgcggccgc gacgacgcga ttcggcgcgc
     3721 gctcgagcag attccaaatg aacggcccgc cgatgcagaa gccgagcacc atgaatttgt
     3781 cgatacccaa gtgatccatg atgccgacgt gatcgtcggt gaacgcgtcc cacggccgct
     3841 cgacttcgag cggccccgtc gactcgccgc cgttggcgtt gcgcagatcg cacgtgatgc
     3901 aacggaactc gttcttgaac gcgtcgatcg ggttgaacgg gtgcgagttg atcaatcccg
     3961 agatcgtcga gttcaagccg ccgcccggaa tgatcagcag cgggaaaccg gagccgcccg
     4021 tgtcgagata acgtatgcgc acggcgccct tctcgtaaaa ggtcccgccg gagctgccgc
     4081 cggccgtttg tgcaagcgtg cgtgaggcgg ccgccgcagc cgccacggcg gcgcccgtct
     4141 tgatcagatc gcgtctcgtt ggagccatac attccccccg aagtaacggc ctggagcgtg
     4201 ccgcccgact aggctcgtcc cctcgccgag gcgagtcaag ccatgccgcg cttgcctcgg
     4261 ctacggactc gcgctatgac tcgccgggca ttggcttcag gggggggtcg tcgcatgcgc
     4321 ctgagttcgt cgttcgccgc cggctgcgcg ctactgctcg ccgcaactac gggcaccgcc
     4381 gcgcagcgag tgctcttcat cggcaacagc ttcacgtacg gcgcggggtc ggcagtgcgc
     4441 ttctaccgca acgactcggt caccgacctc aatgacgaag gcgtgggcgg cgtaccggcg
     4501 ttgttcaagt cgttcgccga cgaagccggc ctcgactacg acgtcgcgct cgagacgcgc
     4561 ggcggcaccg gcctcgattt ccacctggcc gagaagcgcg atctgatcgt gcggcgcggc
     4621 tgggacgtcg tcgtggcgca cggctacagc acgctcgacg ccgatgcgcc gcgcgacccg
     4681 caaaagctca tcgcgacgag ccgcgagctg gccgccgcgc tgcgcagtgc gagtcccgag
     4741 gtcgagctgt ttctgatggc gacttggtcg cgcgccgacc aggtgtatcc ggccgaaggc
     4801 gcctgggcgg ggcggcccat cgaaacgatg gcgaccgata ttcgcgctgc gtacgacgcg
     4861 gctgcggcag ccgcgaaagc caaggtcatt ccggtcggca cggcttggaa cagagcgatt
     4921 cgggacggcg tcgcagatcc gaacccgtac gacggcgtcg aggccggcaa gctcgacctc
     4981 tggacctacg accactatca cgcgagcacg tacggctact atctcgaggc gctcgtcatc
     5041 ttcggcagcg tcaccggccg cgatcc
//
LOCUS       DATLEE010000698         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11033163, whole genome shotgun sequence.
ACCESSION   DATLEE010000698 DATLEE010000000
VERSION     DATLEE010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11033163"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..829)
                     /locus_tag="VM692_15200"
     CDS             complement(74..829)
                     /locus_tag="VM692_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53572.1"
                     /translation="MSERADVVEAVGPSDAPSTAGSALIGARKAYFAITLLILAIVAW
                     GFWGTYYSRLGARGDLPGVVHLHAAAFSLWVLVLVAQASAVAGGRVELHRRLGTAGMF
                     YGGLVFAVGLLVSVGAPAFRVRAGQFPLETGGLVVIYNLTDILLFGAFLALAFAYRNR
                     RDLHTRWIIAATAALCGAALGRVVPGNTPQYLLLWLSPILAMVAVDLVTQRRVHYVPV
                     VSSALLVVAFFKVPLYAAPVWREIGAALLHPFV"
     gene            complement(826..1347)
                     /gene="moaB"
                     /locus_tag="VM692_15205"
     CDS             complement(826..1347)
                     /gene="moaB"
                     /locus_tag="VM692_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HUQ53573.1"
                     /translation="MSEPRELKPLRVAVLTVSDTRNEATDKSGALLVDRLRTAGHTLA
                     AKTIVRDDLYAIRAIVSAWIADAQVEVVITTGGTGLTGRDGTPEAISVLFDKEIDGFG
                     ELFRTISYDEIGASSLQSRAVAGVANATYIFCLPGSSGACATGWDKLIGPQLDFRTRP
                     CNLAELIPRLNER"
     gene            complement(1350..1817)
                     /gene="rimI"
                     /locus_tag="VM692_15210"
     CDS             complement(1350..1817)
                     /gene="rimI"
                     /locus_tag="VM692_15210"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HUQ53574.1"
                     /translation="MAHTDDVAAIAAMSCQLIEHGLAWSWNEERVERCLRNRDCVVLA
                     ARDRRRVIGFAIMEFYAIHAHLNLLAVQPGNRRQGTGRQLLEWLEASARTAGIFTVHL
                     EVRANNVGAQAFYEKLGFRATGRKVAYYDGREDAVRMTHDLTVSTTSPTEPQQ"
     gene            1859..2467
                     /locus_tag="VM692_15215"
     CDS             1859..2467
                     /locus_tag="VM692_15215"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HUQ53575.1"
                     /translation="MTRSAPRDEGRRPRSACILLAAGGSRRLGFPKQLVRRLAHSLLA
                     RALDAARGAVPFGPVIVVLGAEALRMRLVARRAAPSVVVVYNARWQSGLASSLNAGLA
                     ALPRRTTAVLVTLVDQPRVDERSLQRLLRAWRRRPGVAAAASYAGRPGVPAVLPRRYW
                     RAIRALRGDAGARALLRGREITAVDMPEAELDIDTRADVARL"
     gene            2478..>2948
                     /locus_tag="VM692_15220"
     CDS             2478..>2948
                     /locus_tag="VM692_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HUQ53576.1"
                     /translation="MSARFKSVAVVGAGAVGSFYGAMLARAGHRVTLIGRAAHVRAIE
                     QDGLKLDMAGRVEHVRLAASVELAPLRDADLVLFSVKSSATDDVARELAPMLAVNAVI
                     VSLQNGVENAATIAQHVRRIVVPAAVYVATALVEPGLVKHFGRGELVIGALDAAA"
BASE COUNT          491 a          996 c         1004 g          457 t
ORIGIN      
        1 gtcgatctct caggccgtcg ccggccaccc ctcggtccaa agtcgcgagg aatcatagag
       61 caaggcaggg aactcagaca aatgggtgca acagcgcggc gccgatctcg cgccagacgg
      121 gggccgcata taacggcact ttgaagaacg cgacgacgag caacgcgctg ctgacgacgg
      181 ggacgtagtg cacgcgacgc tgcgtcacga gatcgacggc gaccatcgcg agaatcggcg
      241 agagccacaa cagcagatac tgcggtgtgt tgcccggcac gacgcggccg agcgcagcgc
      301 cgcagagcgc cgccgtcgcg gcgatgatcc accgcgtgtg caagtcgcgc cggttgcgat
      361 acgcgaacgc aagcgcgagg aatgcaccga acagcaaaat gtcggtcagg ttgtagatca
      421 cgaccaggcc gcccgtctcg agcgggaact gccctgcgcg gacgcgaaac gccggagcgc
      481 cgacgctgac gagcagcccc acggcgaaga ctaagccgcc gtagaacatg ccggcggtgc
      541 cgaggcgccg gtgcaactcg acgcgtccgc cggcgactgc ggaggcctgc gcgaccagca
      601 cgagcaccca caacgagaac gcggccgcgt gtaggtgcac gacgcccggc agatcgccgc
      661 gtgcgccgag cctcgagtaa tacgtgcccc agaaacccca ggcgacgatc gctagaatca
      721 gcagcgtgat cgcgaaatac gccttgcgcg cgcctatcag cgcagagccg gccgtgcttg
      781 gtgcgtcgga gggaccgaca gcctctacga cgtcggcccg ctcgctcatc gctcgttgag
      841 gcgcggtatg agctccgcga gattgcacgg tcgcgtacgg aagtcgagct gcgggccgat
      901 cagcttgtcc caacccgtcg cgcaggcgcc cgacgatcca ggcaagcaga agatataggt
      961 ggcgttcgca acacccgcga cggcgcgcga ctgcaagctc gacgcaccga tctcgtcgta
     1021 cgagatcgtg cgaaacaatt cgccgaagcc gtcgatctcc ttgtcgaaca acaccgagat
     1081 cgcttcgggc gtgccgtcgc ggccagtgag ccccgtgccg cccgtcgtga tcactacttc
     1141 gacctgcgcg tccgcgatcc acgccgagac gatcgcgcgg atcgcataga ggtcgtcgcg
     1201 cacgatcgtt ttggccgcca gtgtgtggcc tgccgtacgc aggcgatcga cgagcaacgc
     1261 ccccgacttg tccgtcgcct cgttacgcgt gtcggagacc gtgagcacgg ccacgcgcag
     1321 cggcttgagc tctcgcggct cgctcatgct tactgctgcg gctcggtggg cgacgtcgtc
     1381 gagacggtca aatcgtgcgt catgcgcacg gcgtcctcgc ggccgtcgta ataggcgact
     1441 ttgcgccccg tcgcgcggaa ccccagcttc tcgtagaacg cttgcgcgcc gacgttgttg
     1501 gcgcggactt cgagatgcac ggtgaaaatt ccggccgtgc gagccgacgc ctcgagccat
     1561 tcgagcaatt gccggccggt gccctgtcgc cgattacccg gctgcacggc gagtaggttc
     1621 aagtgcgcat ggatcgcgta gaactccatg atcgcgaagc cgatgacgcg ccggcgatcg
     1681 cgagccgcga gcacgacgca atcgcgattg cgcaagcagc gctcgacgcg ctcctcgttc
     1741 cacgaccacg cgagcccgtg ctcgatgagc tgacacgaca ttgcggcgat cgccgcgacg
     1801 tcgtcagtgt gggccaaacc taaccgaacc tgcgtcacgg cttgagtata gtggcaacgt
     1861 gacgagatcg gcaccacgag atgagggccg taggccccgc agcgcttgca ttttgttggc
     1921 agcgggaggc tcgcgccgac tcgggtttcc gaagcagctc gtgcgacggc tcgcgcactc
     1981 gctgcttgcg cgcgctctgg acgccgctcg cggcgccgtg cccttcggcc ccgtgatcgt
     2041 ggtgcttggc gcggaagccc tgcgcatgcg cttggtcgcg cgtcgtgccg cgccaagcgt
     2101 agtcgtggtc tacaacgcgc gctggcagag cgggctggcg agctcgctga acgcgggtct
     2161 cgcggcgctg ccgcgacgta ccactgcggt actcgtgacg ctcgtcgacc agccccgcgt
     2221 cgacgaacgc tccttgcagc ggttgttgcg cgcgtggcgg cgacgtcccg gcgtggcggc
     2281 cgccgcgtcg tacgccggcc ggcccggcgt tccggccgtg ctgccgcgcc ggtattggcg
     2341 cgcgattcgg gcgttgcgcg gcgacgcggg cgcccgtgca ttgctgcgcg gccgcgagat
     2401 cacggcggtc gacatgccgg aagcagagct cgacatcgac acgcgcgccg acgtcgcgcg
     2461 cctataggag acgactcgtg tctgctcgat tcaagtccgt tgccgtggtc ggtgccggcg
     2521 ccgtcggcag cttttatggc gcgatgctcg cgcgcgctgg gcatcgcgtc acattgatcg
     2581 gccgcgccgc ccacgtgcgt gcgatcgagc aggacggact gaagctcgac atggcgggcc
     2641 gcgtggagca cgttcggctt gcggccagcg tcgagctcgc gccgctgcgc gatgcagacc
     2701 tcgtgctatt cagcgtcaag tcatccgcga ccgacgacgt cgcgcgcgag ctggcgccta
     2761 tgctcgctgt gaatgcggtc atcgtcagcc tgcagaacgg cgtcgagaac gcggcgacga
     2821 ttgcccagca tgtgcggcgg atcgtcgtcc cggccgccgt ctatgtcgca acggcgctcg
     2881 tcgagcccgg cttggtcaag catttcggcc gcggcgagct cgtgatcggc gcgctcgacg
     2941 cagcggcc
//
LOCUS       DATLEE010000699        18784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7310532, whole genome shotgun sequence.
ACCESSION   DATLEE010000699 DATLEE010000000
VERSION     DATLEE010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 18784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18784
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7310532"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1382)
                     /locus_tag="VM692_15225"
     CDS             complement(84..1382)
                     /locus_tag="VM692_15225"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ53577.1"
                     /translation="MEQQANGSLRLVLGTLALSVGLALAGTALGQGSQLGGASQDGTS
                     RAGNRNGGDEAPPPTNIDQVTGKVLNEAIELMGMENYSGAQAKLATLKLESLSPYERG
                     KVEQILFNIAYSQDKFDEARRHLQAAIDSGGLNTVEVDGARYQAAQLWMQEEKWKEGA
                     AALEEWFKSTPTMPNSSAYYLLAVAYYQQDAYDKALPPAKKAVELMDKPNENWLQLVS
                     ALHLQREEFKDAVPILQQLIAVAPEKKTYWMQLSSIYGQMEDYPNALAVMQIAHSAGL
                     VTEDSEIRRLADLLLFNDVPYRGAQVLEAAMEKKVVALDDKLYEKLANCWIAAGEFDK
                     SIAPLQRAAEMSSSGDTFIRLGEVQVQREDWPAAITAVQRGVDKGQLKDPGNAQLMLG
                     IAHYNQKEYAAARPFFERARQSERHRNIAESYLQAIRAQG"
     gene            complement(1413..2015)
                     /locus_tag="VM692_15230"
     CDS             complement(1413..2015)
                     /locus_tag="VM692_15230"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HUQ53578.1"
                     /translation="MHALRIPIAIVIGGLFTSSMFWLLWSLVGTAFDVGDRAEAQRIE
                     FSRMRRDTEVATKRDEKVERERPPPTPETPRMAFSAGGIENNVAQLTPIVDARGAMSR
                     MQMTAGSDRDVIPLVRINPDYPPRALSRGLEGWVQVQFTITPTGTVADAKVVNAEPKN
                     IFDDAALKAIARWRYNPKVEGGVAVERVGVQTIIRFQLEQ"
     gene            complement(2040..2447)
                     /locus_tag="VM692_15235"
     CDS             complement(2040..2447)
                     /locus_tag="VM692_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005892521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HUQ53579.1"
                     /translation="MQSRRHAPETEETGIDLAPMLDFTTNLLIFFIITTSFIKEAGVE
                     VFKPSAQTGINRDSGNLLIAIRENGDIWMDRRRIELRDVRPNMERLHIERPDDTVVII
                     ADKESDAGMVAKVMDEVRLAGITTMSIAADPAG"
     gene            complement(2469..2876)
                     /locus_tag="VM692_15240"
     CDS             complement(2469..2876)
                     /locus_tag="VM692_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HUQ53580.1"
                     /translation="MRKRRHSGADHEQTGIDLAPMLDFVTNLLIFFIITAVFVKESGL
                     EVNRPTSFEQPEESDNESIQIQILDTGEVWVDNRAVDVRAVRANVERMSAVNPDSGVL
                     ILAQDKAPTGTLVEVVDQVHLGGIYNITFSTTQ"
     gene            complement(2880..3368)
                     /locus_tag="VM692_15245"
     CDS             complement(2880..3368)
                     /locus_tag="VM692_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HUQ53581.1"
                     /translation="MTLSNDGGPWVIWIFASGVLMWSLIIERYWYFVMVLPKQAANLQ
                     AQWLARTDRTSWCARQIRTAMISRLNGSMTTGFPVMQVLVPMAPLLGLIGTVSGMLEV
                     FDSMALRGNADARSMASGVSAAMICTMTGLAVSITGLYPVHYFRSRAVRETELLADKL
                     SL"
     gene            complement(3394..4788)
                     /locus_tag="VM692_15250"
     CDS             complement(3394..4788)
                     /locus_tag="VM692_15250"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HUQ53582.1"
                     /translation="MIRIKTLLPLAAVLFAAPTLAQNQAATLDDLLKDVQNVRADENQ
                     AFQTRRAEYEAKANQAEKDALLRTAEQNRATLDATSKRLSDTYSANELRISNMNTQLR
                     DKATALGLAEVFGLARQSANDISSILQQSLITAQFVPANGELARDEWLRQFAASRATP
                     TAAELERLWIEIQREMTASGQVAKFRTRVVQPGGEPVESDVIRIGPFTATANGQFLSY
                     LPNLRTLNVLPRQLPGEFMEVAESFTSATSGYARAVVDANRGVLLGLYVERDTPMERV
                     EKGELIGVVIITVGALGALAFIFQLISLVIVRLAVSSQLKHLDRPTANNPLGRVLLAF
                     KGDKAKIEEDADVAELRITEAVLREVPKLERFQAFLRLCVAAGPLLGLIGTVVGMILT
                     FQSITESGSSDPKLMANGIGVAMIATVLGLFVAIALLFANALLNSLSRGVVQILDEQS
                     AGMLAESIEKQRRA"
     gene            complement(4785..5582)
                     /locus_tag="VM692_15255"
     CDS             complement(4785..5582)
                     /locus_tag="VM692_15255"
                     /inference="COORDINATES: protein motif:HMM:NF023358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HUQ53583.1"
                     /translation="MIPFKTKVATATGPRWIHLLVALVPAALAAPAGAQLQEALAQQV
                     QADREAAQSQEELNGIRERTQDAAGRYAQAMADADSLERFNAQLQEQVKSQETEIASI
                     EQQLADIETTNREVQPLMQQMVDTLARFVELDVPFLLDERTARVQRLKDIMNRADITI
                     SEKYRNILEAYQIELEYGRTLSAYEGRLGTGADARTVEFAQLGRVSLMYRTLDGSETG
                     YWDADKKMWVVDESYREAVEEAIRVARRDGAPDLLTVPVPAPQEVRS"
     gene            6303..6701
                     /locus_tag="VM692_15260"
     CDS             6303..6701
                     /locus_tag="VM692_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53584.1"
                     /translation="MTRTAFLALLLATALTIAAPAQVGHPAKGSWSGYWGTSDAAKRR
                     ILLVLDWRDQKITGVINPGPNQVPIEKADLDVNSWTLRLEASMPTDGGGKARFVTTGQ
                     LTNLGSWTTRTYAGTYVFGDERGRFTVTLN"
     gene            6726..7130
                     /locus_tag="VM692_15265"
     CDS             6726..7130
                     /locus_tag="VM692_15265"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53585.1"
                     /translation="MQRTLTALLAAAATALAAHSADAHHSFAAEFDAKSPIELKGTVT
                     KVAWANPHAFFYIDVTTPKGEVENWALELGSPNGLMRRGWTRDSMKIGDVVSVEGSRA
                     KDGSFKGNARSVTLSTGQRLFAGSSQDTAEAN"
     gene            7149..7958
                     /locus_tag="VM692_15270"
     CDS             7149..7958
                     /locus_tag="VM692_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53586.1"
                     /translation="MRNTTTLLYALPLTMALAAASSALAQRGADAAKPPPAPTPRLPD
                     GTVDLGGDGIWEQPWITDFGKQLKGGPDAQIPFLPWTKAMYDYNKSNQVAYDPQGFCL
                     PPGGPRMFGTPYPSEFIQDRNRIVVIFEGGAHVWREIHMDGRPLPKRETLNPTYFGYS
                     VGHWDHDTLVVETTGFNEKTWLNFNGTMHTDELSTVERISRPNRDTLHYEATITDPGA
                     YSEPWTVAWDIPWLAGAELAEYICQENNQFLLDLKDDFGNPFFEDIPTQKP"
     gene            complement(7975..9186)
                     /locus_tag="VM692_15275"
     CDS             complement(7975..9186)
                     /locus_tag="VM692_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ53587.1"
                     /translation="MSASSATASQNATTTAFSVLAAISFCHMLNDMMQSLLPALYPIL
                     KANYALTFAQIGILTFTFQVTASLLQPAIGAFTDKRPQPYSLAMGMGFTFVGLLLLSR
                     AVSYPMLLVAAGLVGMGSAVLHPESSRVARMAAGGRPGLAQSLFQMGGNVGSSLGPLA
                     AVVVLRFGQHSVAWFALAAVVAMVLLSGVGRWYVSHGMARMRAQAARERSGTLSRGRV
                     VGALAILVALMLSKFVYTASMSSYYTFYLIDRFGVEVPQLFLFLYLASVAIGTVVGGL
                     LGDRFGRKFVIWFSILGVLPFTLALPYANLFWTAVLTVPIGMILASAFPAIVVYGQEL
                     VPFRVGTVAGLFFGLAFGLGGVGAALLGQVADVHGIQYVFQLCSFLPVLGLLAVFLPH
                     MATTRRAPAKA"
     gene            9283..9816
                     /locus_tag="VM692_15280"
     CDS             9283..9816
                     /locus_tag="VM692_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53588.1"
                     /translation="MAHVIEPASSSRAKCRSCDQPIAKGELRFGERQPNAFGEGEMTL
                     WFHLPCAAYARPEPFLEVQSTAAAEGQLDSLAAAARFGIEHRRVPRLRGAERATSGRA
                     LCRSCRELIAKGDWRLPLVFFEEYRFNPGGYVHARCAREYFETTDILDRIRHYSPELR
                     EPELTEVAAALAATRAQ"
     gene            9821..11317
                     /gene="hpaB"
                     /locus_tag="VM692_15285"
     CDS             9821..11317
                     /gene="hpaB"
                     /locus_tag="VM692_15285"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HUQ53589.1"
                     /translation="MLSIPAQRNGARRGADLLQRLRERPPALWYRGERVADVTTHTAF
                     SGGVATLAELYDLQWQHAKDCLFDSPASGRKVARSFQLPTTTEELASISRAMGHWARH
                     THGMMGRVPDYLNRAISAYAAGAPFLAEADPRFGANAVRLHEELREADLCMTHSLIPP
                     QANRSVGPARQADPFLAARIKEETDAGFVVRGCRMLATLPISDAIMVLPSTLLKATPD
                     DAPYAFGFVIANDTPGLKFICRESVDYGRSHFDHPLGSRYEEMDAVVVFDDVRVPWEN
                     VLLYRDVERCNSAYVRTGAVVGMAHQVVIKNIAKAEFVLGLAALLVDTIAAEGFQHVQ
                     EKLAELWVTLETMRALKRAAEADAVQDEWGVVRPAWNPLDAARNLFPRLYPRMIEILQ
                     QLGASGLVAMPTEQDLQGPLADDIRRYYQAARADAFERVPLFRLAWDTTISAFAGRQV
                     LYERFFFGDPVRMAGSLLAAHDAEIRSYADRVRELVRGSRDEAFGRVK"
     gene            11403..12545
                     /locus_tag="VM692_15290"
     CDS             11403..12545
                     /locus_tag="VM692_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53590.1"
                     /translation="MFHLGTPVLGTLAASIALALTAPAGAQGFGGGPTATPSAARAVP
                     AETTAAKVRDRNWKAPRTSWGDPSFEGVWSTDDMRSVPTVRPQNFGTRATLTPEEFQQ
                     RASRDEGGADLAANRETFLRNEWGIRTFGYSSRVVDPPSGQIPAMTAAGKARAGNRDR
                     GTFGPGPFEKLEDFTLYDRCITRGVLGSTLPVIYGNGLRIVQNPGSVAISYEMIHDTR
                     IIPLGDRPHLDGAIRQWMGSARGHFEGDTLVVETANFTDQTSIGGNGNGTRHSEALML
                     LERFTRVDPDMIEYIATVTDPVTYTAPFTFRLMITTQPNYETFEYSCHEGNGAVGHSL
                     SGERAFEKEVAEAIAAGQKPPERAQGLGIYGRPREGAEVFDINAGE"
     gene            complement(12604..13014)
                     /locus_tag="VM692_15295"
     CDS             complement(12604..13014)
                     /locus_tag="VM692_15295"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53591.1"
                     /translation="MRVSGWLRVFGGGLLAAVAIPVAAHHSFAAEFDINRPIKLDGVV
                     TKMQFSNPHSWLHIEVTTATGEKQQWAVEGGAPNALIRHGWNRNSVPAGTKVHVEGFQ
                     AKDRTFRASGRDITLPDGSSLFMGQGGAAAPSAE"
     gene            complement(13064..13741)
                     /locus_tag="VM692_15300"
     CDS             complement(13064..13741)
                     /locus_tag="VM692_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53592.1"
                     /translation="MNSANWNLEGHAASQLKQFWRLGAIGAIPAGQSVVKGGKIPYLP
                     EALARRDENRAGWPKTDPEAACYLPGIPRATYLPHPFQIVQGDDDILFVYSFANANRA
                     VHMKDHRTIDEVPVDLWMGWSNGKWEGDTLVVEVIANDDRTWFDRSGNYHSAALKVTE
                     RYTPLDANRIQYEATIEDPETYSAPWTISMHLYKNTEPNAELLEFKCVEFSENLLYNE
                     FLKEPLP"
     gene            14043..15269
                     /locus_tag="VM692_15305"
     CDS             14043..15269
                     /locus_tag="VM692_15305"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ53593.1"
                     /translation="MSNARSAASLLAVAVATLSSPLAFAQREVPFRQGIPVAPSGLAD
                     KPLADGPFDYATGEGQNIRVTVLTKALSYPFTLTFLPDGTMLITQRDGKLRVMRNGRL
                     EPNAVAGGPTSFSTGTSGLPGAVHGYMDIALHPQFAQNQTLYLSYTKPLGENRTTFAV
                     SRAKWTGTALAEAKDIFVAEAGDGGPTPIAFGRDGMLYIATSGGDAQAHDNLGGKVLR
                     VRDDGSVPNDNPFVGKQGYRPEIYTLGHRNSLGLALHPVTGEIWQSENGPNGGDELNI
                     IRAGKNYGWPLVSYGRTYPGPWQAKLPTHEGYEPPAVYWTPAIAVSGFTFYTGDKLKA
                     WTGDVFVGGLRTGEIPGTGKLDRILFNQQMEELRRETLLADLRQRIRDVQQGPDGLLY
                     VITDEDEGAVLRIEPR"
     gene            15433..16479
                     /locus_tag="VM692_15310"
     CDS             15433..16479
                     /locus_tag="VM692_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53594.1"
                     /translation="MQVLPLSRAIFGTLLGTTALLAATSTLPFVGSAQQASSYTVPRT
                     PWGDPDLQGKWPGTEMVGVPMQRDEKLGLRNELTDEEFADRERRAVLQEEADNADFSL
                     ETSGSTPGGDVGGPVSPPPHWVERGEPQRQASLIVDPPNGRMPPFTQAAQDRQAELRA
                     LRQTRGPAESYTDRSNYDRCITRGLAGSILPVIYNNGTQIFQAPGFVAIVNEMIHETR
                     IVPLDGRPHASFAIKMWLGDSRGRFEGDTLVVETTNFTDRTGVGVNGGGQRHSEALKI
                     TERYTRIADDVIDYKMTVEDPETWTAPWTMEFPLKRDDGYGMFEYACHEGNLAMFNIL
                     SGARADDRKAAGSR"
     gene            16476..16856
                     /locus_tag="VM692_15315"
     CDS             16476..16856
                     /locus_tag="VM692_15315"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53595.1"
                     /translation="MKRLPRVIVLVAAVLGVLPAAAHHSWARYDGDHVVTITGSITSV
                     EWASPHVVVHFTVPEANAEPTAWTMEMDPPTLLNRYGLRHDTLKAGMQIKLTGVRARS
                     GAPMMRAVTIEAEDGTVYRVSSRV"
     gene            16864..17859
                     /locus_tag="VM692_15320"
     CDS             16864..17859
                     /locus_tag="VM692_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53596.1"
                     /translation="MHYSTALSALALTSASVALAQAPPPAGFPGTPPPSPPAIIRELT
                     AEPAAIQPGGSTTLRWEALNAYSLTLEPGVGSVATRGAHRVQPLATTTNTLTATGPKG
                     ATTRAVTVTVAGTTPVARAASDEEQRTSIPRLADGKPDLSGLYQADRNLRLVAGVTLV
                     PGAESFRVAAATERDAGTGDDCLPPGVPAATMLPFPLQILQTRDTLAIMYEAYHQFRI
                     VPIGREQAEYLAPAWMGHSVARWDGDTLVVDVRGFNDRTVVAGHRHTEDLRVTERYRR
                     TSYETIEYSAQVEDPNVFATPLRYEGTLTLHPEWEIGEYVCTENNQDYDELFENK"
     gene            complement(17863..18378)
                     /locus_tag="VM692_15325"
     CDS             complement(17863..18378)
                     /locus_tag="VM692_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53597.1"
                     /translation="MMVWKGKAALMSVVISSSALLAGRAAVAHHSFAMYDVGKTYVMT
                     GVVTRVDPNPNHLQIFFAPLNEARDQVLRDDKGEPIVWAVEMDGAGGAARDGISVNGF
                     PRGTIMSVGLHPLRNGFPGGGRGKSGLYKCPADTPPAPGKHCDSVPGSTSHGEGVLPQ
                     PTGAPPPTPAQ"
     gene            complement(18407..>18784)
                     /locus_tag="VM692_15330"
     CDS             complement(18407..>18784)
                     /locus_tag="VM692_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53598.1"
                     /translation="SHSEFEFSSQMQSIEIYTPRKDATGALVGLKQEVILYDDEAFAE
                     PLRIVHNLDKNRELNEGDPFVVMECVPQSFPIDGHTTPVAPGQAVEYRVPDIYGRPWA
                     QIWERYHEEGMERPKERAGRFGL"
BASE COUNT         3362 a         6265 c         5967 g         3190 t
ORIGIN      
        1 ggcgagcgcc aacaggccac cggctgcgac ggcgaacctt cgtttcgaaa tgcacgtcat
       61 gcaaagttcc tagaagaaat cgatcaaccc tgtgcgcgaa tggcctgcaa gtagctctcc
      121 gcgatattcc ggtgccgctc cgactgccgg gcacgctcga agaacggccg cgctgcggcg
      181 tattccttct ggttgtaatg cgcgatgccg agcatcagct gcgcgttacc cggatccttg
      241 agctggcctt tgtcgacgcc gcgttggacg gcggtaatcg cggcaggcca gtcctcgcgt
      301 tgcacttgaa cctcgccaag gcgaatgaac gtatcgcccg agctcgacat ctcggccgca
      361 cgctgcaacg gcgcaatcga cttgtcgaac tcgccggcag caatccagca gttcgcgagc
      421 ttttcataga gcttgtcgtc gagagctacg accttctttt ccatcgcagc ctcgagcact
      481 tgggcgccgc ggtacggaac gtcgttgaac agcaacaagt cggcgagacg gcggatctcg
      541 gaatcctcgg tcacgagccc cgccgagtgc gcgatctgca tgaccgccag cgcgttcgga
      601 taatcctcca tctggccgta gatcgaggag agctgcatcc aatacgtctt cttctcgggc
      661 gcgacagcaa tcagttgctg aaggatggga accgcatcct tgaactcctc gcgttgcagg
      721 tgcaacgccg agaccagctg cagccagttc tcgttcggct tgtccatgag ctcgacggct
      781 ttcttcgccg gcggcagcgc cttgtcgtag gcgtcctgct ggtagtaagc gacggcgagc
      841 aggtaatacg ccgacgagtt cggcatggtc ggcgtgctct tgaaccactc ctcgagcgct
      901 gccgcacctt ccttccattt ctcttcctgc atccagagct gcgcagcctg ataacgcgcg
      961 ccgtcgactt cgacggtgtt caagccgccc gagtcgatcg ctgcctgcag gtgccggcgt
     1021 gcttcgtcga acttgtcctg agagtacgca atgttgaaca ggatttgctc gaccttgccg
     1081 cgctcgtagg ggctcaagct ctccaacttc agcgtggcga gcttcgcctg ggcgccggag
     1141 tagttctcca tgcccatgag ctcgatcgct tcgttgagga ccttgcccgt gacctggtcg
     1201 atgttggtcg gcggcggcgc ttcgtcccca ccgttgcggt taccggctcg gctcgtcccg
     1261 tcttgtgacg cgcccccgag ttggcttcct tgaccaagcg cagtgccggc gagggcaagc
     1321 cccaccgaga gcgccaatgt gccgaggacc aaacgcaaag atccgttagc ctgctgttcc
     1381 ataaattact tctccagctc ctatcggatg atttactgct cgagttggaa gcgaatgatc
     1441 gtctgcacac ccacgcgctc gacggccacg ccgccctcga ccttcgggtt gtagcgccag
     1501 cgcgcgatcg ccttgagtgc cgcgtcgtcg aagatattct tcggctccgc attgacgacc
     1561 ttggcgtctg cgaccgtgcc tgtcggcgtg atcgtgaatt gcacttgcac ccacccttcg
     1621 agcccgcggc tcagcgcccg cggcgggtag tcggggttga tgcgcacgag cgggatgacg
     1681 tcgcggtccg acccggccgt catctgcatg cgggacattg cgccgcgagc gtcgacgatc
     1741 ggcgtcagct gggcaacgtt gttctcgatg ccgcccgcgg aaaatgccat tcgcggcgtt
     1801 tccggcgtcg gcggcgggcg ctcgcgctcg accttctcgt cgcgcttcgt cgcgacttcg
     1861 gtgtcgcggc gcatgcgcga gaactcgatg cgttgcgcct cggctcgatc gccgacgtcg
     1921 aacgccgtgc cgacgaggct ccagagcagc cagaacatcg acgacgtgaa caagccgccg
     1981 atcacgattg cgattgggat tctcaacgcg tgcatagcga tagcctcctg cgtgctcgac
     2041 tagccggccg gatcggccgc gatcgacatg gtcgtgatgc cggccagacg aacctcgtcc
     2101 atgaccttgg ccaccatgcc ggcatccgac tctttgtcgg caatgatcac gacggtgtcg
     2161 tcgggccgct cgatgtggag ccgctccatg ttcggacgaa cgtcgcgaag ctcgatgcgc
     2221 cggcggtcca tccagatatc gccgttctcg cggatcgcga tcagcaagtt gccggagtct
     2281 cgattgatac cggtttgggc cgacggcttg aagacctcga cgccggcttc cttgatgaac
     2341 gaggtcgtga tgatgaaaaa gatcagcaga ttggtcgtga aatcgagcat cggcgcgagg
     2401 tcgatgccgg tttcttccgt ctcgggtgcg tggcgtcgcg actgcatgtc gagctttcct
     2461 cttgccgctt actgcgtcgt gctgaaggtg atgttgtaga tgccgccgag gtgcacctgg
     2521 tcgacgactt cgaccaacgt gcccgtaggt gctttgtcct gcgcgaggat caaaacaccg
     2581 ctgtcgggat tgacggcgct catgcgctcg acgttggcgc gcacggcgcg cacgtcgacg
     2641 gcacggttgt cgacccaaac ctcgccggtg tcgaggatct gaatctggat gctctcgttg
     2701 tccgactctt cgggttgctc gaacgacgtc gggcgattga cttccagccc cgactccttg
     2761 acgaagacag cggtgatgat gaagaagatg agcaggttcg tgacgaagtc cagcatcggc
     2821 gcaaggtcga tgccggtttg ctcgtgatct gctccagagt gtcgtcgctt gcgcatggct
     2881 tagagactca gcttgtccgc gaggagctcc gtctcacgga cggctcggct gcggaagtag
     2941 tggacgggat acagccccgt gatgctgacc gcgagaccgg tcatcgtgca gatcatggcc
     3001 gccgatacgc cgctcgccat gctgcgggca tcggcgttac cgcgtagcgc catcgagtcg
     3061 aacacctcga gcatgccgct gaccgtgccg atcagcccga gcagcggcgc catgggaacc
     3121 aatacctgca tgaccgggaa gcccgtcgtc atcgacccat tgaggcgcga gatcatggcg
     3181 gtgcggatct gccgcgcgca ccacgaggtc cgatcggtcc gagccagcca ctgggcctgc
     3241 aagttggccg cctgtttcgg cagcaccatc acgaagtacc agtaccgctc gatgatcaga
     3301 gaccacatca gcacgccgga ggcgaagatc cagatgaccc acggcccgcc gtcgttcgac
     3361 agcgtcatta cgccccggat gatcggtatt tgctcaagca cggcgttgtt tctcgatgct
     3421 ttccgccagc atgcccgcgc tctgctcgtc gaggatttgg acgacgccac gcgacagcga
     3481 gttcaacagc gcgttggcga acagcagcgc gatagcgacg aacagaccga gcacggtcgc
     3541 gatcatggcc acgccgatac cgttggccat gagcttcgga tcgctcgagc ccgactcggt
     3601 aatgctctgg aacgtcaaga tcatgccgac gaccgtgccg atcaggccca gcaacggacc
     3661 ggcggcgacg cacaggcgca gaaacgcctg gaagcgctcg agcttcggta cttcgcgcag
     3721 cacggcctcc gtgattcgca gctcggcgac gtcggcgtct tcttcgatct tcgccttgtc
     3781 gcccttgaac gcgagcagca cacggccgag cgggttgttc gcggtcggtc ggtcgagatg
     3841 cttgagctgg ctgctgacgg cgaggcgcac gatcacgagg ctgatcagct ggaaaatgaa
     3901 cgcgagggcg ccgagggcgc ctacggtgat gatgaccact ccgatcagct cgcccttctc
     3961 gactcgctcc atcggcgtat cacgctcgac gtaaaggccg agcaacaccc cgcggttcgc
     4021 gtcgaccacg gcgcgtgcat agccggaggt cgcgctggtg aaactctcgg caacctccat
     4081 gaactcgccg ggtagctgcc gcggcagcac gttcagcgtg cgcaggttcg gcaggtacga
     4141 caagaactgg ccgttagccg tcgccgtgaa aggcccgata cgaattacgt cggactcgac
     4201 gggctcgccg cccggctgca cgacgcgcgt acggaatttc gcgacctggc cgctggccgt
     4261 catctcgcgc tgaatctcga tccacaaacg ttcgagctcc gcagccgtcg gcgtcgcgcg
     4321 cgacgccgcg aactggcgca gccactcgtc gcgcgcgagc tcgccattgg ccggcacgaa
     4381 ctgcgccgtg atcaacgatt gctgcaagat cgaggaaatg tcgttcgcgg actgccgcgc
     4441 caagccgaag acctcggcga ggccgagtgc cgtcgccttg tcgcgcagct gcgtgttcat
     4501 attgctgatg cgcagctcgt tcgcggagta ggtgtccgac aagcgcttcg aggttgcgtc
     4561 gagcgttgcg cgattttgct cggcagtgcg cagcaacgca tccttctcgg cttgattggc
     4621 cttcgcctcg tactcggccc ggcgtgtctg gaacgcctgg ttctcgtcgg cccgaacgtt
     4681 ctgcacgtct ttgagcaaat cgtcgagcgt ggcggcttgg ttctgcgcca gcgtcggggc
     4741 agcgaagagg actgccgcga gcggcaacag ggttttgatt ctaatcacga ccgcacctcc
     4801 tgcggcgccg gcacaggcac ggtcagcaaa tccggcgcgc cgtcgcgacg cgccacacga
     4861 atggcttcct cgacggcttc tcgatagctc tcgtcgacga cccacatctt cttgtccgca
     4921 tcccaatagc ccgtctcgga gccatcgagc gtgcggtaca tcagcgagac gcgcccgagc
     4981 tgcgcgaact cgacggtgcg cgcatcggcg ccagtgccga gacggccctc gtaggcgctc
     5041 agcgtgcggc catactcgag ctcgatctga tacgcctcga ggatgttgcg gtacttctcc
     5101 gagatcgtga tgtcggcgcg gttcatgatg tccttgagcc tctggacgcg agccgttcgc
     5161 tcatcgagca agaacggtac gtcgagctcg acgaagcgcg cgagcgtgtc gaccatctgc
     5221 tgcatcagcg gttggacttc gcggttggtc gtctcgatgt ccgccaattg ctgctcgatc
     5281 gaagcgatct ctgtctcttg cgacttcacc tgctcctgca gctgagcgtt gaaccgctcg
     5341 agactgtcgg cgtcggccat cgcctgcgcg taacgcccgg cagcatcctg cgtgcgttcg
     5401 cgaattccgt tcagctcttc ttgcgattgg gcggcttccc gatcggcttg aacttgctga
     5461 gcaagcgcct cttgcagctg tgcgccggca ggtgcagcga gtgcggccgg cacgagcgcc
     5521 accagaaggt ggatccaccg tgggccagta gctgtcgcta ctttggtttt gaaaggaatc
     5581 atgacaacaa gcgcctcctg aagattacct gcacacgact cgcgccagtc gactggtgga
     5641 cgagccattt agttcgaacc aacccaaaac gactaggcag cctccgcccg agcgaagtgc
     5701 cgccgacaaa ccaatgatcc acgaaggact gtgcgcctac aaccacgcgc gcgccagtaa
     5761 gcgtcggctc tgcatgccgc acgcgagatg taccgatgag ctaaatcacc gtacacaagc
     5821 tccccctgcc agacctgaag ggactgctga atcccttcgc ccgaataacg cgggtctcgg
     5881 ccatagcacc caaatctagt tcgacttatt cgtcgcctgc gtcctgcgga agttgccgcc
     5941 gaccagcggc tctcccccac cgccggcgtc gtgagccaag ctccacgcaa gcgacgatgt
     6001 cttagtgtag tcaaatgtgt ctgcgcatgc ctaggggtca gagggtatcg gctctttctg
     6061 cggcgcctcg gtccgtcgac tcgatcctgc cgcgccgacg cacgggcgcg ccggcgctcc
     6121 gtcgtagacg aactcgtcag gacggctcgc gacacggctc cacctgcacc atccgtaccg
     6181 tcagttccgc cgttgcagcg agttcgcgcc aactcgcgca ttcgtcagtt atgtcgcccc
     6241 gagaccgagt actcgcagtg ctatattcgc acttgggatt ttcgcacggt gcgccgctac
     6301 tcatgacgcg aacggctttt ctcgcattgc tattggccac ggcactgacc atcgccgcac
     6361 ccgcgcaggt cggccatccg gcgaagggtt cgtggagcgg ctactggggc accagcgatg
     6421 ccgcgaagcg acgcatcctc ctcgtgctcg attggcgcga tcaaaagatc accggcgtca
     6481 tcaatccggg cccaaaccag gtgcctatcg agaaagccga cctcgacgtc aactcgtgga
     6541 cgctgcggct cgaggcctcg atgccgaccg acggcggcgg caaagctcgt ttcgtcacaa
     6601 ccggccaact caccaatctc ggctcctgga cgacgcgtac ttacgcaggc acttatgtgt
     6661 tcggcgacga gcgcggccgg ttcaccgtca cgctgaattg aaacttccgc taagaggagg
     6721 gacgcatgca gaggacattg accgcgctcc tggccgccgc agcgacggcg ctggccgcgc
     6781 actcggccga cgcgcaccac tcgttcgccg ccgagttcga cgccaagagc ccgatcgagc
     6841 tcaagggcac ggtaaccaag gtcgcttggg ccaaccctca cgccttcttc tatatagatg
     6901 tgacgacgcc taagggcgaa gtcgagaact gggcgctcga attgggtagt cccaacgggt
     6961 taatgcgccg gggctggacg cgggactcga tgaagatcgg cgacgtcgtg tccgtcgaag
     7021 gctcgcgcgc gaaggacggt agcttcaagg gcaacgcgcg ctccgtgacc ctgtcgacgg
     7081 gccagcgcct gttcgccggc tcgagccagg acaccgccga agccaactga cacgcgagga
     7141 cttcaaccgt gcgcaacacc accaccctgc tctacgcgct tccgctgacg atggcgcttg
     7201 ccgccgcgag ctccgcgctt gcacaacgcg gagccgatgc tgccaaaccg ccgccggcgc
     7261 cgacgccgcg cctaccggac ggcacggtcg atctcggcgg cgacggcatc tgggagcagc
     7321 cttggattac ggacttcggc aagcagctga agggcggccc cgacgcgcag atcccgtttc
     7381 tgccgtggac gaaagccatg tacgactaca acaagtcgaa ccaggtggct tacgacccgc
     7441 agggtttctg cctgccgccc ggcgggccgc gcatgttcgg cacgccctat ccgtcggagt
     7501 tcattcagga ccgcaatcgc atcgtcgtga ttttcgaggg cggcgcgcac gtgtggcgcg
     7561 agatccacat ggacggtcgg ccgctgccga agcgcgagac tctgaatccg acgtacttcg
     7621 gctattcggt cggccactgg gatcacgaca cgctcgtcgt cgagacgacg ggtttcaacg
     7681 agaagacttg gttgaacttc aacggcacga tgcacaccga cgagctgtcg accgtcgagc
     7741 gcatcagccg gccgaaccgc gacacgttgc actacgaggc gacgattacc gatcccggcg
     7801 cttactcgga gccatggacg gtcgcttggg acattccatg gctcgccggt gcggagctcg
     7861 cggagtacat ctgccaggag aacaaccagt tcttgctcga cctcaaggac gacttcggca
     7921 atccgttctt cgaggacatc ccgacccaaa agccctgagc cgagccggcg cgcttcacgc
     7981 cttcgcgggc gcgcggcgcg tcgtcgccat gtgcggcaaa aacaccgcga gcaacccgag
     8041 cacgggcaag aacgagcaca gctggaagac gtactgaatt ccgtgcacgt cggcgacctg
     8101 accgagcaac gccgcgccga cgccgccgag cccgaacgcg agcccaaaga acaagcccgc
     8161 gaccgtgccc actcgaaacg gcacgagctc ttgcccgtag acgacgatcg cgggaaacgc
     8221 cgacgcgagg atcatgccga tcggcacggt cagaacggcc gtccaaaaca gattggcgta
     8281 cggcagcgcg agcgtgaacg gcaacacgcc gaggatcgag aaccaaatga cgaacttgcg
     8341 cccgaagcgg tcgccgagca gaccgccgac gaccgtgcct atcgctacgg acgcgagata
     8401 gaggaacaag aacaactgcg gcacttcgac gccgaatcgg tcgatcagat aaaacgtgta
     8461 gtagctcgac atgctggccg tgtagacgaa cttcgacagc atcaacgcga cgagaatcgc
     8521 cagcgcgccg acgacgcgac cgcgcgacag cgtgcccgag cgctcgcgcg cggcctgcgc
     8581 gcgcatccgc gccatgccgt gcgagacgta ccaacggccg acgcccgaca gcagcaccat
     8641 cgctacgacg gcagccaagg cgaaccaggc gacgctgtgt tggccgaacc gcagcacgac
     8701 gacggccgcg agcggcccca gcgacgaccc gacgttcccg cccatctgga acaacgactg
     8761 cgcgaggccg ggccgcccgc cggccgccat acgcgcgacg cgtgacgact cggggtgcag
     8821 cacggcggag cccatcccca cgaggccggc cgccacgagc agcatgggat agctgacggc
     8881 gcgcgacagc aacagcaggc cgacgaacgt aaagcccatg cccatggcga gcgagtacgg
     8941 ctgcggccgc ttgtccgtga acgcgccgat cgcgggttgc aacagcgagg ccgtcacctg
     9001 aaaggtgaac gtcaggatgc cgatctgcgc gaacgtcagc gcgtagttgg ctttcagaat
     9061 cgggtacagc gccggcagca acgactgcat catgtcgttc aacatgtggc agaagctgat
     9121 cgcggcgagc acggagaacg ccgtcgtcgt cgcgttctgc gaagcggttg cggaagacgc
     9181 gctcacgagt caaagcctcg gtacgggagt tcgggaagcc taccagacgc cccggcgcgc
     9241 tgtgagcaga cccgatcggt cggggccctc tatactctcg gcgtggctca cgtcatcgaa
     9301 cccgcctcga gcagccgcgc gaagtgccgc agttgcgatc aaccgatcgc caaaggcgag
     9361 cttcggttcg gcgagcggca accgaatgcg ttcggcgagg gcgagatgac gttgtggttc
     9421 cacctaccct gcgccgcgta tgcgcggccg gagccgttcc tcgaggtgca gtccacggca
     9481 gccgcggagg ggcagctcga ctcgcttgcc gcggccgcgc gcttcggcat cgagcaccgc
     9541 cgcgtgccgc gcctgcgcgg cgcggagcgc gcgacgtcgg ggcgcgctct ttgccgtagt
     9601 tgccgcgagc tcatcgccaa aggcgactgg cgattgccgc tggtcttctt cgaggagtat
     9661 cggttcaacc ccggcggcta cgttcacgcg cgttgcgcgc gcgagtactt cgagacgacg
     9721 gacatcctcg atcgcatccg ccactacagc cccgagctcc gcgagccgga gctgaccgaa
     9781 gtcgcggcgg cgttggctgc gacgcgcgcc caatgagctg atgctctcca taccggcaca
     9841 acgcaacggc gcgcgccgcg gcgcggacct gctgcaacgc ctgcgcgaac ggccgccggc
     9901 tctctggtat cgcggcgagc gtgtcgccga cgtgacgacg cataccgcct ttagcggcgg
     9961 agtcgcgacg cttgcagagc tctacgatct gcagtggcag cacgcgaagg actgcctgtt
    10021 cgactcgccg gcgagtggcc gcaaagtcgc gcgctcgttc cagttgccga cgacgacgga
    10081 agagctcgcg agcatcagtc gtgcgatggg ccattgggcg cgccacacgc acggcatgat
    10141 gggccgcgtg ccggactatc tgaatcgcgc gatcagcgcc tacgccgcag gcgcgccgtt
    10201 tctggccgag gctgacccgc gcttcggcgc gaacgcagtg cgcctgcacg aggagttgcg
    10261 cgaggcggat ctctgcatga cgcactcgct gattccgccg caagccaatc gcagcgtcgg
    10321 gcctgcgcgc caggccgacc cgttcctcgc ggcgcgaatc aaagaggaga ccgatgcagg
    10381 tttcgtcgtg cgcggctgcc gcatgctcgc gacgctgccg atctccgacg cgatcatggt
    10441 gctgccgtcg acgttgttga aagccacgcc cgacgacgcg ccgtacgcgt tcgggttcgt
    10501 gatcgccaac gacacaccgg ggctcaagtt catttgccgc gagagcgtcg actacgggcg
    10561 ctcgcacttc gaccatccgc tcggctcgcg ctacgaagag atggacgccg tcgtcgtctt
    10621 cgacgacgtg cgggtgccgt gggaaaacgt gctgctgtat cgcgatgtcg agcgctgcaa
    10681 ttccgcgtat gtccggacgg gcgccgtcgt cggcatggcg catcaggtgg tgatcaagaa
    10741 catcgcaaag gccgagttcg tcttgggatt ggctgccctg ctcgttgaca cgatcgcggc
    10801 cgaaggcttt caacacgttc aagagaagct tgcggagctc tgggtgacgc tcgagacgat
    10861 gcgcgcgctc aagcgcgcag ccgaggcgga cgcggtgcaa gacgagtggg gagtcgtacg
    10921 ccctgcgtgg aacccgctcg acgcggcgcg caatctgttt ccgcgcttgt acccgcgcat
    10981 gatcgaaata ttgcagcagc tcggcgccag cggcttggtc gctatgccga ccgagcagga
    11041 cttgcaaggc ccgctcgcgg acgacattcg ccgctattac caggcggcgc gcgcggacgc
    11101 cttcgagcgc gttccgctgt tccgcttggc atgggatacg acgatctccg cgtttgccgg
    11161 ccggcaagtc ctttacgaac gcttcttctt cggcgatccc gtgcgcatgg cgggctcgtt
    11221 gctcgcggcg cacgacgcgg aaatccgcag ctatgcagac cgtgttcgcg agctcgtccg
    11281 cggctcgcgc gatgaagcgt tcggccgagt aaaatagcgt cttggccaat ggtggataca
    11341 aacccttgac ctattcttcg gtcccttcgg cgtagcgccg cttggggtca gggagcttca
    11401 gcatgtttca tttaggaaca ccggttctcg gcacgctggc cgcgagtatc gcgctggcgc
    11461 tgacggcgcc ggcgggggct caaggcttcg gcggcggtcc gacggcgacg ccgtccgcgg
    11521 cacgcgccgt tccagccgag acaaccgccg ccaaagttcg cgaccgtaac tggaaagcgc
    11581 cgcgcacgtc gtggggcgac ccgagcttcg agggcgtctg gagcaccgac gacatgcgta
    11641 gtgtgccgac cgtgcggccg cagaacttcg gcacgcgcgc gacgctgacg cccgaagagt
    11701 tccagcagcg agcgagccgc gacgaaggcg gcgcggacct agccgccaac cgcgagacgt
    11761 tcctgcgcaa cgagtggggc atccgcacgt tcggctattc gtcgcgcgtc gtcgacccgc
    11821 cgagcggcca gattccggcg atgacggcgg ccggtaaagc acgcgccggc aatcgcgacc
    11881 gcggcacgtt cggcccgggc cccttcgaga agctcgagga cttcacgctt tacgatcgct
    11941 gcatcacgcg cggcgtgctc ggctcgacgt tgcccgtcat ctacggcaac ggcctgcgca
    12001 tcgtccaaaa cccgggctcg gtggcgatca gctacgagat gatccacgac acccgcatca
    12061 tcccgctcgg cgaccggccg cacctcgacg gcgccatccg acaatggatg ggcagcgcgc
    12121 gcggtcactt cgagggcgat acgctcgtcg tcgagacggc aaatttcacg gatcagacca
    12181 gcatcggcgg caacggtaac ggcacgcgcc acagcgaggc attgatgctc ctggagcggt
    12241 tcactcgcgt cgaccccgat atgatcgagt acatcgcgac ggtaacggat ccagtcactt
    12301 acaccgcacc gttcacgttc cgactgatga tcacgacgca gccgaactac gagacattcg
    12361 agtactcgtg ccacgaaggc aacggcgccg tcggtcactc gttgagcggc gagcgtgcgt
    12421 tcgagaagga agtggccgag gcgatcgccg ccggccagaa gccgccggag cgagcgcagg
    12481 gtttgggcat ctacggccgg ccgcgcgagg gcgcggaagt cttcgacatc aatgccggcg
    12541 aataaaaaaa cggcgctgcg gcttgcggcc gcagcgccgt tgcgtcttga agcgctaggc
    12601 gagctactcg gcgctcggcg ccgccgctcc accctgaccc atgaacagcg acgagccgtc
    12661 cggcaacgtg atgtcacggc cgctcgcgcg gaacgttcga tctttcgcct ggaagccttc
    12721 gacatgcacc ttcgtgccgg cggggaccga gttgcggttc caaccatgac gaatcagcgc
    12781 gttcggcgcg ccgccttcga ccgcccattg ctgcttctcg ccggttgccg tcgtcacttc
    12841 gatgtgcaac cacgaatgcg ggttgctgaa ctgcatcttc gtcacgacgc cgtcgagctt
    12901 gatgggccga ttgatgtcga actcggccgc gaacgaatgg tgcgcggcga cgggtatcgc
    12961 cactgcagcc agcaggccgc caccgaagac tcttaaccaa ccactgactc gcattgctcc
    13021 tctcctaagc tccccaatcg attcgtgcga agcgcgccca atctcagggc agcggctcct
    13081 tgagaaattc gttgtacagc agattctccg agaattcgac acacttgaac tcgagcagct
    13141 ctgcgttcgg ctccgtgttc ttgtaaagat gcatcgagat ggtccacggt gcggaatacg
    13201 tctccgggtc ctcgatcgtc gcttcgtact gaatgcgatt cgcatcgagc ggcgtgtagc
    13261 gctcggtcac cttcagcgcc gcgctgtggt agttgccgga gcgatcgaac caagtgcggt
    13321 cgtcgttcgc gatgacttcg acgaccagcg tgtcgccttc ccacttgccg ttcgaccagc
    13381 ccatccacaa gtcgacgggc acctcgtcga tcgtacggtg atccttcatg tgcacggcgc
    13441 gattcgcgtt cgcgaacgaa taaacgaaca gaatgtcgtc gtcgccctgc acgatctgga
    13501 acgggtgcgg caagtacgtg gcgcgcggaa tgccgggcaa atagcacgcc gcttcgggat
    13561 cggtcttcgg ccaaccggcg cggttctcgt cgcggcgcgc gagcgcctcg ggcaggtacg
    13621 ggatcttgcc gcccttgacg acgctctggc cggccgggat cgcgccgatc gcgcctagac
    13681 gccaaaactg cttcagctgg gaagccgcat ggccttcgag gttccagttg gccgagttca
    13741 tggcctgcca gataccgttc aggttcggat ggccgccaac gcggcgcgcc gcttgagcgt
    13801 ttgcagtcag gggaacggca cttccgaatg cgagcgccgc aaccaacgaa accaacaacc
    13861 cctgccgcat cttgcccccc aaaacgatag tggttcttcg aagccggcga gaatcatagc
    13921 gaactcgcta cggtgggtgc agcgccggaa tccaaattta gctgagcgcc ccagccgcgg
    13981 cggctttaag ggcccgctgc agtcgcccta tactgcattt tccaacctgg gggtcacttt
    14041 ccatgtccaa cgctcgctcc gctgcgtcgc tgctcgctgt cgccgttgcc acgctgtcct
    14101 cgccgctcgc atttgcgcag cgcgaggtgc cgtttcgcca gggaattccc gtcgcaccct
    14161 cgggcctcgc cgacaagccg ctcgcagacg gcccgttcga ctacgccacc ggcgaaggtc
    14221 agaacatccg cgtgacggtg ctgacgaagg cactgtcgta tccgttcacg ctgacgtttc
    14281 tgcccgacgg cacgatgttg atcacgcagc gcgacggcaa gttgcgcgtg atgcgcaacg
    14341 gcaggctcga gccgaacgct gtcgccggcg gaccgacgtc gttctcgacc ggcacgtcgg
    14401 ggttgcccgg cgccgtgcac ggctacatgg acatcgcgct gcatccgcag ttcgcgcaga
    14461 accagacgct gtacttgagc tacacgaagc cgctcggcga gaaccgcacg acgttcgcgg
    14521 tctcgcgcgc caagtggaca ggaacggcgc ttgccgaggc gaaggacatc ttcgtcgccg
    14581 aagcaggtga cggcgggccg acgccgatcg cattcggccg cgacggcatg ctgtacatcg
    14641 cgacgagcgg cggcgacgcg caagcgcacg acaacctcgg cggcaaagtg ctgcgcgtgc
    14701 gcgacgacgg cagcgtgccg aacgacaacc cgttcgtcgg caagcagggc tatcgcccgg
    14761 agatctacac gctcggccac cgcaactcgc tggggctcgc gttgcacccg gtcacgggcg
    14821 agatctggca gagcgagaac ggcccgaacg gcggcgacga gctcaacatc atccgcgccg
    14881 gcaagaacta cggttggccg ctcgtcagct acggccgcac gtatcccggc ccgtggcaag
    14941 cgaagttgcc gacgcacgaa gggtacgagc cccccgccgt ctactggacg ccggcgatcg
    15001 ccgtgtcggg cttcaccttt tatacgggcg acaagctcaa ggcgtggaca ggcgacgtgt
    15061 ttgtcggcgg cctgcgcacg ggtgagattc cgggcaccgg caagctcgac cgcatcctct
    15121 tcaaccagca gatggaggag ctacggcgtg agacgttgct ggccgatctg cgccaacgca
    15181 ttcgcgacgt gcagcaaggc cccgacggct tgctctacgt catcaccgac gaggatgaag
    15241 gcgcggtgct gcgcatcgag ccgcgctgag cgcgaggagc tcacccatga ggcgccgagc
    15301 gttggccact gccctagcgg ctctcgcgtg cggcgccggc gcggcggcgc aagatcgcct
    15361 gtcgtcgggg caccgcgccg aagagcgcgc tgccgccggc cgttgacgac ggataacgac
    15421 ggagaacagc cagtgcaagt gttgccactc tcgcgcgcaa tcttcggcac gttgctcggc
    15481 accactgccc tgctcgccgc caccagcacg ctgcccttcg tcggcagcgc gcagcaagcg
    15541 agcagctaca ccgttcctcg cacgccatgg ggcgacccgg acctgcaagg caaatggccc
    15601 ggcacagaga tggtcggcgt gccgatgcag cgcgacgaaa agcttgggct ccgcaacgag
    15661 ctgaccgatg aagagttcgc cgaccgcgag cgccgcgcag tgctgcaaga ggaagccgac
    15721 aacgccgact tcagcctcga gacctccgga agcacacccg gcggcgacgt cggcggcccg
    15781 gtctctcctc cgccgcactg ggtcgagcgc ggcgagccgc aacgccaagc gtcgctgatc
    15841 gtcgatccgc cgaacggccg catgccgccc ttcacgcagg cggcacaaga tcgtcaagcc
    15901 gagctacgcg cgttgcgcca gacccgcggg ccggcggaat cctacacgga tcgcagcaac
    15961 tacgatcgct gcatcacgcg cgggctcgcg ggctcgatcc taccggtcat ctacaacaac
    16021 ggcacgcaga tctttcaggc gccgggcttc gtcgcgatcg tcaacgagat gatccacgag
    16081 acgcgcatcg tgccgctcga cggccgaccg cacgcgagct tcgcgatcaa gatgtggctc
    16141 ggcgattcgc gcggccgctt cgaaggcgac acgctggtcg tcgagacgac gaacttcacg
    16201 gaccgcacgg gcgtcggcgt caacggcggc ggccagcgcc acagcgaagc gctgaagatc
    16261 acggaacgct acacgcgcat cgccgacgac gtcatcgact acaagatgac ggtcgaggat
    16321 cccgaaacgt ggacggcgcc gtggacgatg gagtttccgc tgaagcgcga cgacggctac
    16381 ggcatgttcg agtacgcgtg ccacgagggc aacctcgcga tgttcaacat cctgagcggc
    16441 gcgcgcgccg acgaccgcaa agcagcaggc tcgcgatgaa gcgactcccg cgcgtcatcg
    16501 tgctcgtcgc ggccgtgctc ggcgttctgc ccgccgccgc ccatcactcg tgggcgcgct
    16561 acgacggcga tcacgtcgtc acgatcacgg gcagcatcac gtcggtcgaa tgggcgagcc
    16621 cccacgtcgt cgtgcacttc accgtgccgg aggcgaacgc ggagcctacg gcctggacga
    16681 tggagatgga tccgccgacg ctgttgaacc gctacggctt gcggcacgac acgctgaagg
    16741 ccggcatgca gatcaagctg accggcgttc gcgctcgttc cggcgcgccg atgatgcgcg
    16801 ccgtgacgat cgaagccgag gacggcaccg tctaccgcgt ctcgagccga gtttaagctc
    16861 agcatgcatt actcgacggc cttgtcggcg ttggcgctca cgagcgcgag cgtcgcgctg
    16921 gcccaggcgc ctccaccggc cgggttcccc ggcacaccgc caccgagccc gccggccatc
    16981 attcgcgagc tgacggccga gcccgcggca attcagccgg gaggctcgac gacgttgcgc
    17041 tgggaagccc tgaacgccta ttcgctgacg ctcgagcccg gcgtcggcag cgtcgcgacg
    17101 cgcggtgcgc accgcgttca gccgctagcg acgaccacga acacgttgac ggcgacgggg
    17161 cccaaaggcg cgacgacccg ggcggtgacc gtcacggtcg ccggcacgac acccgtggcg
    17221 cgcgccgcat cggacgaaga gcaacgcacc tcgattccgc gcctcgccga cggcaagccg
    17281 gatctctcgg ggctctacca ggcagaccgc aacttgaggc tcgtcgccgg ggtgacgttg
    17341 gtgccgggcg ccgagtcgtt ccgcgtcgcc gcggcgaccg aacgcgacgc cggcaccggc
    17401 gacgactgcc taccgcccgg cgtgccggcg gcgacgatgc tgcccttccc gctgcagatc
    17461 ctgcaaacgc gcgacacgct cgcgatcatg tatgaggcct atcaccaatt ccgcatcgtg
    17521 ccgatcgggc gcgagcaggc ggagtacctg gcgccggcct ggatgggcca ctccgtcgcg
    17581 cgctgggacg gcgacacgct cgtcgtcgac gtgcgcggct tcaacgatcg aaccgtcgtc
    17641 gcgggccacc ggcacaccga ggacctgcgc gtcaccgagc gctatcggcg cacgtcgtac
    17701 gagacgatcg agtactcggc gcaggtcgag gacccaaacg tattcgcgac gccgctgcgc
    17761 tacgaaggca cgctgacgct gcaccccgag tgggagatcg gcgagtacgt ctgcaccgag
    17821 aacaaccagg actacgacga gctgttcgag aacaagtgaa agctattggg caggcgtagg
    17881 cggcggcgct cccgttggtt gcggcagcac gccttcgccg tgcgacgtcg agccgggcac
    17941 cgagtcgcag tgttttccgg gggccggcgg agtatcggcc ggacacttgt acaagcccga
    18001 cttgccgcgg ccgccgcccg gaaaaccgtt tcgcagcgga tgcaggccga cgctcatgat
    18061 cgttccgcgc gggaagccgt tgacgctgat gccgtcgcgc gcagcgccgc cggcgccgtc
    18121 catctcgacg gcccagacga tgggctcgcc tttgtcgtcg cgcagcactt ggtcgcgagc
    18181 ttcgttcagc ggtgcgaaga agatctgcag atgattcggg ttgggatcga cgcgcgtcac
    18241 gacgcccgtc atcacgtagg tcttacctac gtcgtacatc gcgaacgagt ggtgcgcgac
    18301 ggcggctcgc cccgccagca gcgccgagct cgagatcacg acgctcatca acgccgcctt
    18361 gcctttccac accatcattg catcggtcct cgcattgctt tgctcgtcac aaaccgaagc
    18421 ggcccgcgcg ttccttgggc cgctccattc cctcttcgtg gtagcgctcc cagatctgtg
    18481 cccagggccg gccgtagatg tccggcacac ggtattcgac cgcttgcccc ggcgcgaccg
    18541 gcgtcgtgtg gccgtcgatc ggaaagcttt gcggcacaca ctccatgacg acgaacggat
    18601 ccccttcgtt gagctcgcgg ttcttgtcga ggttgtgcac gatgcgcagc ggctccgcga
    18661 acgcctcgtc gtcgtagagg atcacttcct gcttgaggcc gacgagcgcg ccggtcgcat
    18721 ccttgcgcgg cgtgtagatc tcgatcgact gcatctggct cgaaaactcg aactccgagt
    18781 gcga
//
LOCUS       DATLEE010000700         6345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11034922, whole genome shotgun sequence.
ACCESSION   DATLEE010000700 DATLEE010000000
VERSION     DATLEE010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11034922"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..1290
                     /locus_tag="VM692_15335"
     CDS             349..1290
                     /locus_tag="VM692_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ53599.1"
                     /translation="MKRRRFISLLGGAGAAGLLGGRELSLRAQTAPGPLAMTELRSGL
                     QLVTGAGGNVVARAGTDGLTLVDSGSPESATALRKALTERLGSAPVTTLFNTHWHLDH
                     TGGNDALVEGHDATIIAHENTRLWMSTEFDVEWEEKHYERRGRASWPNKTFFSSDKQP
                     LTVDLGEKITYGHLSEAHTDGDIYVQFPDTNVIVAGGAVTAGRYPLIDYITGGWIGGM
                     ADATTKLLGMMDAETLVVPDLGPVQRRADLEAQVKMLQTVRERIEGIALQGRGIEDMV
                     AEAITKDFDARYAGDPAKFIEHAYESMWWSRLRGIVA"
     gene            1331..3736
                     /locus_tag="VM692_15340"
     CDS             1331..3736
                     /locus_tag="VM692_15340"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ53600.1"
                     /translation="MAALALTACAESPEEALASRWPMLQQYCTECHNDAEAAGNMSLE
                     GVTPAEVAANPQKWENVVRRLRGSVMPPPGGEHPKIEQVNEFVAALEANLDAAAAARG
                     AAPGRVMLHRLNRVEYATAVHDLLGVDVDPTRLLPPDASSDGFDNVAEVLRVTPTYLD
                     QYIAAARAISIEAVGSPAAKLARSEYVSDNRNHSVHVDGLPLGTRDGLAVVHHFPSDG
                     DYVFNLRVSSTPGEELSAYRRSWLAYEHTVIMTIDGVRVFEGKLGGEDDLRDLDRYQI
                     VTVNAIKDRFRNIRLPVKAGDREVVATFVARARSESDYILQGFVPGEGTPDVPLMLGF
                     DVTGPYEPTGISSGMASRDRIFVCTPASAAEELPCAERILSNLARLAFRQTATETDIE
                     RLLSFYREGAGEGGFEAGIQKGVLAILSSTKFLYRAEPGGPPEGIKAGESYAVSDLEL
                     ASRLSFFIWSQGPDEELLALAESKRLSDPRVYELEIERLLADPRSKSLVTNFAFQWLS
                     VRDVDVVEPDPKLYPNFDQDLRKAFEREMELFADSILRQPGHSVVELLTAPYTFVNER
                     LARHYGIDGVRGDRFRRVELDDPNRWGIFGKGSVLLATSYPDRTSPVLRGAWVMEHVL
                     GVPPTPPPPGVETNLTPSLETPRSVRERLALHRTQTSCNHCHGVIDPLGQALENFNAI
                     GEWRTFERDSGAAIDPTGSLANGRRANSPTELREAIAAEPEKFVQTVAQNLLTYALGR
                     RVEYYDMPAVRAIVREAAESDYSFAAIVKGVASSAPFRMRSAPEAVQETVAAAEQGGS
                     SGD"
     gene            3751..5097
                     /locus_tag="VM692_15345"
     CDS             3751..5097
                     /locus_tag="VM692_15345"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ53601.1"
                     /translation="MFITKKHLSRRTFLRAGSATIGLPLLSAMVPAATALAQTAAAPK
                     PRLGFFYLPHGAIMANTLHGAEMNRWTPDKVGRDFDLKKILEPFAPHQKYLTVFSGLG
                     NRTAESPSVHAIFPGTWLTCVAPPRSQTPNGGISIDQIAARHIGQETPLPSLELATEE
                     RGGSGGCDGTYGCSYSRTISFRTPTTPLPMEADPGKVFEKIFGRGTTDAERGAIAADF
                     SSVLDLVTTEIKDLERGLGAEDRVMVNDYLDSVRELERRIQLLSARDLSNVDLPEIPV
                     ASPAFDERIKLMFDLLVAAYQTNSTRIATFMMAAEVSNMSYAHIGVPDAFHPLSHHAD
                     NKNSLEKLAQVQRYHSQVFADFLGKLAAIPDGDAGSLLDSSIFLYGSNMSNSNKHDSF
                     PLPTLVVGGANGKIKGNQHLRVADRTPIANLHYTLMLRAGVPVDKVGDSTGEIAEV"
     gene            5100..>6345
                     /locus_tag="VM692_15350"
     CDS             5100..>6345
                     /locus_tag="VM692_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ53602.1"
                     /translation="MGCIRGAVILVGLTLAAWSATASAELVDAARRGDRGAVLAELAR
                     GGDAKAKAPDGTTALHWAVYNDDVELVQRLLGAGAEPNAANDYGATPLSEAAVTANTA
                     VIKALLEAGADAKATGKDGETALMVLARSSNVDAARLLIEHGADVNAVEAWRGQTAVI
                     WAAAQKQPDMIRLLVEHGADPNARSKVNDWDRQVSGEKRRQYRPFGGLTALMYAAREG
                     CADCARALVEGGADIDLAGSRNMTPLIMALENMNFDTAAYLLEAGAAVDVWDWWGRTP
                     LYMAVDLNTVPHGGRPDRLSTDSTPAIALVERILERGANPNSQLKMLPPYRERGNDRG
                     CDSMLVTGATPLLRAAKTFDTAAMKALIAHGARLDLPNASGITPLMAAAGYGSLECDI
                     RGYGPGIPHYLTADVEQKSIAAL"
BASE COUNT         1116 a         2100 c         2141 g          988 t
ORIGIN      
        1 ggccggcgtc agattcgccg gcggtgcctg ctggccttcg gcggcgcggt tcgcgagcgc
       61 gagcacgatg accgacgtcg caactgcaac gccgcctgcg atgcagcggc gaaacgacaa
      121 tgaacgcgtc atgacggatt cctccggttg atgcagtgag cttagctgct ggccgagacc
      181 atcgcagcct tgagcggctc cagctttcta aagcgacgtg ccgccgaaaa cggacgccac
      241 ggcgtagccg gcagcgcgcc ctggcagtat tatttctggt gctaaatgcc tgatgcgcaa
      301 cgcaatgagg catggcggag gggacagcgt tttgacgggc ccagagtctt gaagcgccgc
      361 cgattcataa gcctgctcgg cggggccggc gcggccggtc tgctgggcgg ccgcgagctg
      421 tcgctgcgcg cgcaaacggc gccggggccg ttggccatga cggagttgcg ctccggcttg
      481 caactcgtca cgggcgccgg cggcaacgtc gtcgcgcggg ccggcaccga cgggctaacg
      541 ctcgtcgaca gcggctcccc tgagagcgcg acggcgctgc gcaaggcgct gaccgagcgg
      601 ctcggctccg cgccggtcac gacgctcttc aatacgcact ggcacctcga tcacaccggc
      661 ggcaacgacg cgctcgtcga ggggcacgac gcgacgatca ttgctcacga gaacacccgg
      721 ctctggatga gcacggagtt cgacgtcgag tgggaagaga agcattacga gcgacgcggg
      781 cgagccagct ggccgaacaa gacgttcttc agctccgaca agcagccgct gacggtcgac
      841 ctcggcgaga agatcaccta cggtcacctc tctgaagctc atacggacgg cgacatctac
      901 gtgcagtttc cggataccaa cgtcatcgtc gcgggcggag ccgtcacggc gggccgctat
      961 ccgctgatcg actacatcac gggcggttgg atcgggggca tggccgatgc cacgacgaag
     1021 ctgctcggca tgatggatgc ggaaacgctc gtcgtgcccg acttgggtcc cgtgcagcgg
     1081 cgcgcggatc tcgaggcaca agtgaagatg ctgcagacgg tccgcgagcg catcgagggc
     1141 attgcgctgc aagggcgcgg catcgaggac atggtcgccg aggcgatcac gaaggacttc
     1201 gacgcgcgct atgccggcga ccccgcgaag ttcatcgagc acgcgtacga aagtatgtgg
     1261 tggagccggc tgcgtggaat cgtggcgtaa ggaaatgcta gagaaacgaa tactgactgg
     1321 ctcggcattc ttggcggcgc tcgctctcac ggcgtgcgcg gagtcgcccg aggaggcgct
     1381 cgcgagtcgc tggccgatgc ttcagcagta ctgcaccgag tgtcacaacg acgccgaggc
     1441 cgccggcaac atgtcgctcg aaggcgtgac gccggccgag gtggcagcca atccgcagaa
     1501 gtgggagaac gtcgtccggc gcttgcgcgg cagcgtcatg ccgccgcccg gcggcgagca
     1561 tcccaagatc gagcaggtca acgagttcgt cgccgcgctc gaggccaatc tcgacgccgc
     1621 cgccgcggct cgcggcgcgg cgccggggcg cgtcatgttg cataggctca atcgcgtcga
     1681 gtacgcgacg gccgtgcacg atctgctcgg cgtcgacgtc gacccgactc gcttgctgcc
     1741 gccggacgcg agcagcgacg gtttcgacaa cgtcgccgag gtgttgcgcg tcacgccgac
     1801 ctatctcgat cagtacatcg ccgccgcgcg tgcgatcagc atcgaggccg tcggcagccc
     1861 ggcggcgaag ctggcgcggt ccgagtacgt ctccgacaac cgcaatcact ccgtgcacgt
     1921 cgacggcctg ccgctcggca cgcgcgacgg cttggccgtc gtgcaccact tcccgtcgga
     1981 cggcgactac gtgttcaacc tgcgcgtgtc gtcgactccc ggcgaagagc tcagcgccta
     2041 tcggcgcagc tggctcgcgt acgagcacac cgtgatcatg acgatcgacg gcgtgagagt
     2101 gttcgagggc aagctcggcg gcgaagacga cctgcgcgac ctcgaccgct atcagatcgt
     2161 cactgtcaac gcgatcaagg accgtttccg gaacatccgc ctgcccgtga aagcaggcga
     2221 tcgtgaagtc gtggcgactt tcgtcgcgcg cgcgcgatcc gagtccgact atatcctcca
     2281 gggcttcgtg cccggcgagg gcacgcccga cgtaccactg atgcttggct tcgacgtgac
     2341 gggcccctac gagccgacgg gcatcagcag cggcatggcg agccgtgacc gtatcttcgt
     2401 ttgcacgccg gcgagcgcgg ccgaggaatt gccgtgcgcg gaacgaatct tgagcaacct
     2461 cgcgcggctc gcgttccgcc aaacggccac tgaaaccgac atcgagcggt tgctgtcgtt
     2521 ttaccgcgaa ggggccggcg aaggcggctt cgaggccggc atccaaaagg gcgtgctggc
     2581 catcctgtcg agcacgaaat tcctttatcg tgccgagccg ggcggcccgc cggaaggaat
     2641 caaggccggc gagtcctacg ccgtctcgga tctcgagctc gcgtcgcgcc tgtcgttctt
     2701 catctggagc caaggtcccg acgaggagtt gctcgcgctg gccgagagca agcgcttgag
     2761 cgacccgcgc gtctacgagc ttgagatcga gcgcttgctc gccgaccctc gctctaagtc
     2821 gctcgtcacg aacttcgcgt tccagtggct cagcgtgcgc gacgtcgacg tcgtcgagcc
     2881 ggacccgaag ctgtacccga acttcgatca agatctgcgc aaggcgttcg agcgcgaaat
     2941 ggagctgttc gccgacagca tcttgcgcca gccgggccac agcgtcgtcg agctgttgac
     3001 ggccccgtat acgttcgtca acgagcgcct cgcgcgccac tacggcatcg acggggtgcg
     3061 cggcgaccgc ttccgccgcg tcgagctcga cgacccgaac cggtggggca tattcggcaa
     3121 gggcagcgtg ctgctcgcga cgtcataccc cgatcgcacg tccccggtat tgcgtggcgc
     3181 ctgggtcatg gagcacgtgc ttggggtccc gccgactccg ccgccgccgg gcgtcgagac
     3241 gaatctcacg ccgtcattgg aaacgccgcg ctcggtgcgc gaacgcctcg cgctgcaccg
     3301 cacgcagacg tcgtgcaacc actgccacgg cgtcatcgac ccgctcggcc aagcgctcga
     3361 gaacttcaac gcgatcggcg agtggcgaac gttcgagcgc gacagcggcg cggccatcga
     3421 cccgactggt agcctcgcga acggccgcag ggccaacagt ccgacagaac tgcgcgaagc
     3481 catcgcggcc gagcccgaga agttcgtgca gaccgtggcg cagaacctct tgacgtacgc
     3541 gttgggacgg cgcgtcgagt actacgacat gccagccgtg cgtgccatcg tgcgcgaggc
     3601 ggccgagagc gactattctt ttgctgcaat cgtcaaaggc gtcgcgagca gcgcgccgtt
     3661 tcgaatgcgt agtgcgccgg aagccgtgca agagacggtt gcggcggcgg agcagggtgg
     3721 gagttccggc gactagccgg ggaggcatcg atgttcatca cgaagaagca tttgtcgcga
     3781 cgcacgtttc tgcgcgcggg cagcgccacg atcgggctgc cgctgctgag cgcgatggtc
     3841 ccggctgcca ctgcgctcgc gcagacggct gccgcgccga agcctcggct cggtttcttc
     3901 tatctaccgc acggcgcgat catggcgaac acgctgcacg gcgccgagat gaatcgctgg
     3961 acgcccgaca aagtggggcg cgacttcgac ctcaagaaga tcctcgagcc gtttgctccg
     4021 catcagaagt acctaacggt gttcagcggt ctcggcaacc gcacggccga gagcccatcg
     4081 gtgcacgcga ttttccccgg cacgtggctg acctgcgtag cgccgccgcg ctcgcagacg
     4141 ccgaacggcg gcatctcgat cgatcagatc gcggcgcggc acatcggcca agaaacgccg
     4201 ctgccgtcgc tcgagctcgc gacggaggaa cgaggcggca gcggtggttg cgacggcacg
     4261 tatgggtgca gctatagccg cacgatctcg ttccgcacgc cgacgacgcc gttgccgatg
     4321 gaggcggacc ccggcaaggt cttcgagaag atcttcggcc gcggcaccac ggacgccgag
     4381 cgcggcgcga tcgccgcgga tttttcgagc gtgctcgacc tcgtgacgac cgagatcaag
     4441 gatctcgagc gcggtctcgg cgccgaggac cgcgtcatgg tcaacgacta tctcgacagc
     4501 gtccgtgagc tcgagcggcg aatccagctg ctcagcgcgc gcgatctgtc gaacgtggac
     4561 ctgccggaga ttcccgtcgc gtcgccggca ttcgacgagc gcatcaagct catgttcgat
     4621 ctgttggtcg cggcctatca aaccaactcg acgcgcatcg cgacgttcat gatggccgcc
     4681 gaggtcagca acatgtcgta cgcgcacatc ggcgtccccg acgcgttcca cccgctctcg
     4741 catcacgccg acaacaagaa ctcgctcgag aagctcgcgc aggtccagcg ctatcacagt
     4801 caggtgttcg cggacttcct cggcaagctc gcggcgattc ccgacggcga tgccggctcg
     4861 ttgctcgaca gctcgatctt cctctacggc agcaacatga gtaacagcaa caagcacgac
     4921 tcgttcccgt tgccgacgct cgtggttggc ggtgcgaacg gcaagatcaa gggcaaccag
     4981 cacttgcggg tcgccgatcg cacgccgatc gcgaacctgc actacacatt gatgctgcgc
     5041 gccggggtgc ccgtcgacaa agtcggcgac agcaccggcg agatcgcgga ggtctgacgg
     5101 tgggctgcat acgcggagcg gtcatcctcg tcgggctaac gctcgcagct tggagcgcga
     5161 cagcgagcgc cgagctcgtc gatgcggcgc ggcgcggcga tcgcggcgcc gtgttggccg
     5221 agctcgcgcg cggcggggac gcgaaagcga aagcgccgga cggcacgact gcgttgcact
     5281 gggcggttta caacgacgac gtcgagctcg tgcagcgcct gctcggcgcc ggcgcagagc
     5341 cgaacgcggc caacgactac ggcgcgacgc cgctcagcga agctgccgtg accgcgaata
     5401 ccgccgtcat caaagcgctg ctcgaggccg gcgccgatgc gaaggccacg ggtaaggacg
     5461 gcgagaccgc gctgatggtg ctcgcgcgca gcagcaacgt cgacgcggcg cggctcctca
     5521 tcgagcacgg cgccgacgtc aacgccgtcg aagcgtggcg cggtcaaacc gcagtgattt
     5581 gggccgcggc gcagaaacag cccgacatga tcaggctgct cgtcgagcac ggcgccgatc
     5641 cgaacgcgcg ctcgaaggtc aacgattggg atcggcaggt ctccggcgag aagcgtcggc
     5701 agtatcggcc gttcggcggc ctgacggcgc tcatgtacgc ggctcgagag ggctgcgcgg
     5761 attgcgcgcg ggcgcttgta gaaggcgggg ccgacatcga tctcgccggt tcgcgcaaca
     5821 tgacgccgct gatcatggcg ctcgagaaca tgaatttcga caccgccgcg tatctgctcg
     5881 aggccggcgc cgccgtcgac gtctgggatt ggtggggtcg cacgccgctc tacatggccg
     5941 tcgacttgaa cacggtcccg cacggcggcc gtcccgatcg gctgtcgacc gactccacgc
     6001 cggcgatcgc actggtcgag cgcatcctcg agcgcggcgc caacccgaac tcgcagctga
     6061 aaatgctgcc gccgtaccgc gagcgcggca acgaccgcgg ttgcgactcg atgctcgtga
     6121 cgggcgcgac gccgctgttg cgcgcggcca agacgttcga cacggccgcg atgaaggcgt
     6181 tgattgcgca cggtgcgcgg ctcgacttgc cgaacgcgag cggcatcacg ccgctgatgg
     6241 ccgccgcagg ttacggctcg ctcgaatgcg acatccgtgg ttacggtccc ggcattccgc
     6301 attacttgac cgccgacgtc gagcagaaat cgatcgcggc gctgc
//
LOCUS       DATLEE010000701         2126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7313875, whole genome shotgun sequence.
ACCESSION   DATLEE010000701 DATLEE010000000
VERSION     DATLEE010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7313875"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1820
                     /locus_tag="VM692_15355"
     CDS             <1..1820
                     /locus_tag="VM692_15355"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUQ53603.1"
                     /translation="SGVTLAQANADLARLLPVWLDRFPFPGGAKGHEIYLDGWRITPT
                     VRTLKDDVVGAIGDVLWVVMAMMGIVLLIACANVTNLLLVRGERRSQEFGVRAALGAG
                     TWPIARALVAEALLLAAAGGAFGILLGYGALQFMLALAPPQLPRLDSIALDSRGVGFA
                     LALALAAGILVSFAPVLRAARVRVSTALRSARGASLGRVQQRAQNTLVVGQVALALVL
                     LVSSGLMVRTFQAMRAVEPGFTGAREIETFRINSPPTLVPQDRDVFVQQRAIIDALEA
                     IPGVSSAGFVNGLPLEGGSAYWDGISIEGAEYAVGGDLALRVFRTMSPGYLETMGTRV
                     IAGRDLEWADLDETRAVVLISESLAREKWTTPEAALGKRIRTAGGSGTPWREIVGVVE
                     DVRLIGADQPAPTIVYWPSLMKDFYALAPSFVERGVALAVRSPLAGTPALERQIEQAV
                     WSVNLSLPIARMRTMQDIYDRSLARTSFTLVMLVVAAAAALVLGLVGLYGVLSYTVSL
                     RRREIAIRLALGARQQDVRRRFVRQGVVLAGIGVAAGLLGAVVATRLMSALLYEVQAV
                     DPLTYGAVAAGLTLVAALASYLPARRAASVAPTESLAAE"
BASE COUNT          274 a          718 c          750 g          384 t
ORIGIN      
        1 ggtccggcgt cacgctcgcg caagcgaatg ccgatctcgc gcgcctcttg ccggtttggc
       61 tcgatcgttt tccgttcccc ggcggcgcca aaggtcacga aatctatctc gacggctggc
      121 ggatcacgcc gacggtgcgg acattgaagg acgacgtcgt cggcgccatc ggcgacgtgc
      181 tctgggtcgt catggcgatg atgggcattg tgttgctgat cgcgtgcgcc aacgtgacga
      241 acttgctgct cgtgcgcggc gagcgccgca gccaggagtt cggcgtgcgg gcggcgctcg
      301 gcgccggcac ttggccgatt gcgcgagcgc tggtcgccga agcgctgttg ctcgcggccg
      361 ccggcggcgc tttcggcatc cttctcggct acggggcgct gcagttcatg ctggcgctcg
      421 cgccgccgca gttgccgcgc ctcgactcga ttgcgctcga ctctcgcggt gtggggttcg
      481 ctctcgcgct ggcgctcgcg gccggcatcc tcgtgagctt cgcgcccgtc ctgcgggccg
      541 cgcgcgttcg agtttcgacg gcgttgcgca gcgcgcgcgg cgccagctta ggccgcgtgc
      601 agcagcgcgc gcaaaacacg ctagtcgtcg ggcaagtcgc cctcgcgctc gtgctgctcg
      661 tcagctcggg tttgatggtc cgcacgttcc aagcgatgcg cgcggtcgag ccgggcttca
      721 cgggcgcgcg tgagatcgag acgttccgca tcaactcgcc gccgacactc gtgccgcagg
      781 atcgcgacgt gttcgtgcag cagcgcgcga tcatcgacgc gctcgaggcg atccccggcg
      841 tgagctcggc cggcttcgtc aacggcttgc cgctcgaagg cggctccgct tactgggacg
      901 gcatcagcat cgagggcgcc gagtacgccg tgggcggcga cctcgcgcta cgcgtctttc
      961 gcacgatgtc gcccgggtac ctcgagacga tggggacgcg cgtcatcgcg ggccgcgacc
     1021 tcgagtgggc cgacctcgac gagacgcggg ccgtcgtgct gatctccgag agcctggcgc
     1081 gcgagaaatg gacgacgccg gaagccgcgc tcggcaagcg cattcgcacg gccggcggct
     1141 cgggaacgcc atggcgcgag atcgtcggcg tcgtcgagga cgtgcgcttg atcggcgccg
     1201 atcagcccgc gccgacgatc gtctattggc ccagcctcat gaaagatttc tacgcgctcg
     1261 cgccgtcctt cgtcgaacgc ggcgtcgcgc ttgccgtgcg cagcccgctt gcgggcacgc
     1321 ccgcgctcga gcgccagatc gagcaagccg tttggtccgt aaatctgagc ttgccgatcg
     1381 cgcggatgcg gacgatgcag gacatctacg atcgctcgct agcgcgtacg tcgttcacgc
     1441 tggtcatgct cgtcgtcgcg gcggcggccg cgctcgtgct tggcctcgtc ggcctctacg
     1501 gcgtgctgtc gtacacggtg tcgctgcggc ggcgcgagat cgcaatccgc ttggcgctcg
     1561 gcgcacggca gcaagacgtg aggcggcgct tcgttcgcca aggcgtcgtg ctggccggca
     1621 tcggcgtcgc cgcggggctc ctcggcgccg tggtcgcgac gcgtttgatg tcggcgctgc
     1681 tctatgaagt gcaggctgtc gatcccctga cttacggcgc cgtcgccgcc gggctcacgc
     1741 tcgttgcggc actcgcgagt tatctgccgg cgcgccgcgc cgcgagcgtg gccccgaccg
     1801 aatcgctggc ggccgaatga cggaagtggc gcgtagcacc gcaactcgcg ctttgccaca
     1861 agacccttcg cggccgcgtc tgtgcactat cgcggtgcaa aatctctccg gggagggatt
     1921 ttgtgagaca ggcgggcgcg tggcgagtgc gacccacgga gtgatccaat ggagaagctg
     1981 atcggcgacg ttcgctacgc agctcgagtg ctggcgcgaa cgcccggctt cacgctcgtc
     2041 gtcgtgctga cactcgcgct cggtatcggc gcgaacagcg ccgtgttctc ggcgatcgac
     2101 tcgatcctgt tgcagccgct cggttt
//
LOCUS       DATLEE010000702         3925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7314227, whole genome shotgun sequence.
ACCESSION   DATLEE010000702 DATLEE010000000
VERSION     DATLEE010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7314227"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VM692_15360"
     CDS             complement(<1..1241)
                     /locus_tag="VM692_15360"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HUQ53604.1"
                     /translation="MPNEDLDRREFLAGAAAVAGAASGAWQAGAQSATDLCFRSATEL
                     VELIRTRQVSAREVMAAHLERIARVNSQVNAIVAKLDDDQCLALADAADRRAGNGEPL
                     PPLHGLPTAFKDLQPAVGFPYTRGSPIYKDAMPTEDSVFVERLRRAGVIPIGKTNVPE
                     FGMGSHTYNKVYGTTLNPYDHTKSAGGSSGGAGAALATGMLPIADGRDLGGSLRNPGN
                     FNNIVGFRPSVGLTPTWPTNFPLLGFSVNGPLARTVADIGLLMSVMAGPDARDPSLLP
                     VDPATFRAPLERDFRGTRVAWSPDLGGLPLDRRVRAVLDSQRKTFEDLGLVVEEATPG
                     LTDADSIFLTLRAFRSAASYGPLLAQYRDLLKPEAITEIELGQSLTTAAVAQAMVRQG
                     QLMDRMRRFEERYAFTLCAVN"
     gene            1353..1904
                     /locus_tag="VM692_15365"
     CDS             1353..1904
                     /locus_tag="VM692_15365"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HUQ53605.1"
                     /translation="MSRHRRTRLLLAAVMASAAGAALAQAPASAPAQAPLPDGYMRAP
                     AVRAGLAPKMRATETTTPAHVFDVAFTTGDEILSGLTDLAIKNKITSGYITGLGGLSG
                     ALLGFGDPPINAFAKIPVDEKCELVSLTGHIQMRDGVPTVHLHAIVALKDGSTKAGHV
                     FEAHVAPIAEISVVATSVAGAAH"
     gene            1925..2386
                     /locus_tag="VM692_15370"
     CDS             1925..2386
                     /locus_tag="VM692_15370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HUQ53606.1"
                     /translation="MRTLVAWFAALIAAAAPAVVQAQSADRAADEAAVRAVIKAFIDT
                     REANDAAGLAALLTSDVDQRQTSGNMRNGRDAVVAGSMATQQSTGGRRITLDSLRFVG
                     ADVAIADGRYDSVGRSDGSDQRMLTSMVLRREGAAWKIAAIRNMLPSTPQP"
     gene            2410..3066
                     /locus_tag="VM692_15375"
     CDS             2410..3066
                     /locus_tag="VM692_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008483105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family NAAT transporter"
                     /protein_id="HUQ53607.1"
                     /translation="MSTIEEGLLYILSTVGALLPIVNPLSAVGLVVGITQELTEAERR
                     QQIARACIYMFGILTAFLVAGGVIMSFFGISIPGLRIAGGLIVGYIGFRMLFPDTSGI
                     SRQERDEAAHKPDVSFTPLAMPMLSGPGSIGVVVGIASTAQDGDHVVLNYALVAMGIA
                     VTALISYVVLRAATRLNQVLGATGMNAIARLMGFLLVCIGVQFVINGIGDVVQRNFQA
                     "
     gene            3063..3251
                     /locus_tag="VM692_15380"
     CDS             3063..3251
                     /locus_tag="VM692_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53608.1"
                     /translation="MIQFKPGLVQEDGTVTNDSTAQFLSGFMREFYTFIVRVYTVLPR
                     NVDAQAGRSRGAREELRH"
     gene            3300..3917
                     /locus_tag="VM692_15385"
     CDS             3300..3917
                     /locus_tag="VM692_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HUQ53609.1"
                     /translation="MRILVLFAHPRLRQSVVQRAMLAAITGLPDITVRDLYADYPDLM
                     IDARREQAELLAHDVLVLQHPLYWYSSPAIIKEWLDIVLEEGWAYGVGGKRLHGKFLL
                     NAISTGGPQEAYHGKGRNRFPVRSLLAPFDQTAYLCGMGWLEPFVVHAGRKLDAAALS
                     GRCERYRDLLVGLRDGRLEPQQHLAQGFTLPSNFERAAARHANAT"
BASE COUNT          621 a         1325 c         1320 g          659 t
ORIGIN      
        1 tgatttacgg cgcacagcgt gaacgcatag cgttcctcga agcgccgcat gcgatccatg
       61 agctggccct gacgcaccat ggcttgcgcg acggcggccg tcgtcagcga ctgaccaagc
      121 tcgatttcgg taatcgcttc cggcttcagt aggtcccggt actgcgcgag cagggggcca
      181 tagctagccg ccgagcggaa cgcgcgcagc gtcagaaaga tcgagtcggc atcggtcaga
      241 cccggagtgg cttcctcgac gacgagacct aagtcctcga acgtcttgcg ctgcgagtcg
      301 agcacggcgc gcacgcgccg gtcgagcggc agcccgccga gatcggggga ccaggcgacg
      361 cgcgtaccgc ggaagtcgcg ctcgagcggc gcgcgaaacg tcgcgggatc gacgggtagc
      421 aacgatggat cgcgcgcatc gggcccggcc atcacgctca tcagcaagcc gatgtcggcg
      481 accgtgcgcg cgagcggccc gttgaccgag aagccgagca gcggaaagtt cgtcggccac
      541 gtcggcgtca gaccgacgct cggccggaag ccgacgatgt tgttgaagtt gccggggttg
      601 cgcagcgagc cgccgagatc gcggccgtcg gcgatcggca gcatccccgt tgcgagcgcc
      661 gctcctgcgc cgccgctcga gccgccggcg ctcttcgtgt ggtcatacgg gttcagtgtc
      721 gtgccgtaga ccttgttgta cgtgtgcgag cccatgccga actcgggcac gttcgtcttg
      781 ccgatcggta tgacgccggc gcgccgcagg cgctcgacga acacggaatc ctcggttggc
      841 atcgcgtcct tgtagatcgg cgagccgcgc gtgtacggga agccgacggc cggctgcaaa
      901 tccttgaacg ccgtcggcag gccgtgcaaa ggcggcagcg gctcgccatt cccagcgcgc
      961 cggtctgcag catcggcgag cgcgaggcat tgatcgtcat cgagcttggc gacgatggcg
     1021 ttgacttggc tgttgacgcg agcgatgcgc tcgaggtgcg cggccatgac ctcgcgcgcc
     1081 gagacttgcc gcgtgcggat cagctcgacg agctccgtgg cactcctgaa gcataaatcc
     1141 gtggcgctct gggccccggc ctgccatgcg ccgctcgcgg cccccgcgac tgccgccgct
     1201 ccggccagga attcgcgccg atccagatct tcgttcggca tggttgcccg ctcccgtacg
     1261 cccctggcgc gcgtccacac tagtgccgag agagacatct gcgcaaccga ggggcagaat
     1321 accgaaagcc tatagcgctc cgggggaacg ccatgagccg ccatcgccgc acacgtttgt
     1381 tactcgccgc cgtaatggcc agcgccgctg gcgccgcgct ggcccaagcg cccgcgtccg
     1441 cgccggccca ggccccgctg cccgacggct atatgcgcgc gccggcggtg cgagccgggc
     1501 ttgcgccgaa gatgcgcgcg accgagacga cgacgccggc ccacgtcttc gacgtcgcgt
     1561 tcacgacggg tgacgagatc ttgtcggggc tcacggatct cgcgatcaag aacaagatca
     1621 cgtcgggcta catcacgggc ttgggcggat tgtcgggtgc gctgctcggc ttcggcgacc
     1681 cgccgatcaa cgcgttcgcg aagattcccg tcgacgaaaa gtgcgagctc gtctcgttga
     1741 ccggtcacat ccagatgcgc gacggcgtcc cgaccgtgca cttgcacgcg atcgtcgcgc
     1801 tgaaggacgg cagcacgaag gcggggcacg tgttcgaagc gcacgtggcg ccgatcgccg
     1861 agatctccgt cgtcgcgacg tctgtcgcgg gcgccgccca ttgaccgcga cacggaggca
     1921 cgccatgcgc acgctcgtcg cctggttcgc agcgctcatc gcggcagccg cgccggccgt
     1981 cgtccaggca caaagcgccg accgcgcggc ggacgaagcg gcggtgcgcg ccgtcatcaa
     2041 ggctttcatc gacacgcgcg aggccaacga cgcagccggc ctcgccgcgc tattgacgtc
     2101 ggacgtcgac cagcgccaga cgtcgggcaa catgcgcaac ggccgcgacg ccgtcgtcgc
     2161 gggctcgatg gcgacgcagc agagcacggg cggccggcgg atcacgctcg actcgctgcg
     2221 cttcgtcggc gccgacgtcg cgatcgccga cgggcgctac gacagcgtcg gccgcagcga
     2281 cggctccgat cagcgcatgc tgacctcgat ggtgctgcgc cgcgaaggcg ccgcgtggaa
     2341 gatcgcggcc atccgcaaca tgctcccgag cacaccgcag ccctgatgcc gttctaggcg
     2401 cgccccgcgt tgagcacgat cgaggaaggc ctgctgtaca tcttgagcac ggtcggggca
     2461 ttgctgccga tcgtgaaccc gctgtccgcc gtcgggctcg tggtcggcat cacgcaggag
     2521 ctgaccgaag ccgaacgacg ccagcagatc gcgcgggcct gcatctatat gttcggcatc
     2581 ctgacggcgt tcctcgtcgc gggcggcgtg atcatgagct tcttcggcat ttcgattccc
     2641 ggtttgcgta tcgcgggcgg tctgatcgtc ggctacatcg gcttccgcat gctgttcccc
     2701 gacacgagcg gcatctcgcg ccaggagcgc gatgaagccg cgcacaagcc cgacgtctcg
     2761 ttcacgccgc tcgcgatgcc gatgctgagc ggcccaggct cgatcggcgt cgtcgtcggc
     2821 attgcgtcga cggcgcagga cggtgaccac gtcgtgctga attacgcgct cgtggcgatg
     2881 ggcatcgccg tgacggcgtt gatttcgtat gtcgtgctgc gcgccgcgac gcgcctcaat
     2941 caggtgctcg gtgccacggg catgaacgcg atcgcgcggc tcatgggctt cttgctcgtg
     3001 tgcatcggcg tgcagttcgt catcaacggc attggcgacg tggtgcagcg caatttccaa
     3061 gcttgatcca gttcaagccg ggcctcgtcc aggaggatgg cacggtcacg aacgattcga
     3121 ctgcgcagtt tctgagcggc ttcatgcggg agttctacac gttcatcgtg cgtgtctaca
     3181 ccgtgctgcc ccgcaacgtc gacgcgcagg ccggccgctc gcgcggcgca cgcgaagagc
     3241 ttcggcactg acgacgctcg ccctttgctt tttcgcgcag gcgtcggcca tgatcgggca
     3301 tgcgcattct cgtcttgttc gctcacccgc ggttgcggca gtccgtcgtg caacgcgcga
     3361 tgttggcggc gatcacgggg ctgcccgaca tcacggtccg cgacctctac gccgactatc
     3421 ccgatctcat gatcgacgca cgccgcgagc aagccgaact gctcgcgcat gacgtgctcg
     3481 tgctgcagca cccgctgtac tggtactcgt cgccggcgat catcaaagag tggctcgaca
     3541 tcgtgctcga ggaaggttgg gcgtacggcg tcggcggtaa gcggttgcac ggcaaatttt
     3601 tgctgaacgc gatctcgacg ggcgggccgc aggaggccta ccacggcaaa ggccgcaatc
     3661 gtttcccggt tcgcagcttg ctcgcaccgt tcgatcagac ggcctatctc tgcggcatgg
     3721 gttggctcga gccgttcgtg gttcatgccg ggcgcaagct cgacgccgcc gctctcagcg
     3781 gccgctgcga gcgttatcgc gacttgctcg tggggctacg cgacgggcgg ctcgagccgc
     3841 agcaacatct cgcgcaaggc ttcacgctgc cgtcgaactt cgagcgcgcg gcggcgcggc
     3901 acgccaatgc aacttgatga aattc
//
LOCUS       DATLEE010000703         2596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3606155, whole genome shotgun sequence.
ACCESSION   DATLEE010000703 DATLEE010000000
VERSION     DATLEE010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3606155"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /gene="tcuB"
                     /locus_tag="VM692_15390"
     CDS             complement(<1..349)
                     /gene="tcuB"
                     /locus_tag="VM692_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735527.1"
                     /note="with CitA (TcuA) catalyzes the oxidation of
                     tricarballylate to cis-aconitate; originally thought to be
                     involved in citrate utilization; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization protein TcuB"
                     /protein_id="HUQ53610.1"
                     /translation="MQATELTREGARVLSVCNACRYCEQYCPAFQAMEDRLTFATADL
                     NYLANLCHGCGECLYACQYAPPHEFAINVPRTFAKLRVQSYEQYAWPAALGAAFRRQG
                     IMTALLLVGAMVAL"
     gene            complement(336..1730)
                     /gene="tcuA"
                     /locus_tag="VM692_15395"
     CDS             complement(336..1730)
                     /gene="tcuA"
                     /locus_tag="VM692_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HUQ53611.1"
                     /translation="MPDAPAYDVLVIGGGNAGLTAALTARRLGARVLVLECAPRPFRG
                     GNSRHTRNLRCAHDVPTEILTETYTQEELLEDLRRVNGGETDEALAALVAARSGECVP
                     WMTGLGARFQSSLRGTLQLARTNAFFLGGGTALMNSYYAAAERLGVDVRYDAEVVELA
                     LGDGSFHSATVRSGGATTALRAQAVVLAAGGFEANLEWLERAWGPAARNFLIRGTPYN
                     TGTVLKLMLDAGAAPIGDARECHAVAIDGRAPKFDGGIVTRLDCVPLGIVVNAQAERF
                     YDEGEDFWPKRYAIWGKLVARQPGENAYSIIDAKVRGRFMPSVYPPIEAASLSELAGK
                     VDLPPERLEATVAAFNRAVQPGAFDHTTLDDCRTAGLTPAKSHWAQRLDTPPYAAYPL
                     RPGITFTYLGVRVDSSARVLAASGAPFPNVYAAGEIMAGNVLRKGYIAGIGMTIGTVF
                     GRIAGEGAARHASH"
     gene            complement(1723..>2596)
                     /locus_tag="VM692_15400"
     CDS             complement(1723..>2596)
                     /locus_tag="VM692_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HUQ53612.1"
                     /translation="VAVGLFAVGEALYAASRHQLGAQPEIVVLRGSLWMTRDEWSRSW
                     KPWLRGTAIGFPLGVLPMGGSVIPTFLSYLAEKRLSAHPEEFGRGAIEGVAGPEAANN
                     ASTAGVLVPLLTLGVPSSATAAILLAAFQQYGLQPGPLLFSARPDLVWTLIASLFIGN
                     VMLLVLNLPLAGVWARVMLLPRSLLFGGVLVLASLGAYTLNRSLLDVALLYVVGLLGL
                     VLRLCKVPLVPAVLGLVLGPMAEQQFRRALAISDGDVTVFVTRPISAALLLAALLLLV
                     APWLLDLRRRRKHA"
BASE COUNT          454 a          907 c          887 g          348 t
ORIGIN      
        1 gcaacgcaac cattgcgccg acgagcagca aagccgtcat gattccttgg cggcggaacg
       61 ccgcgccgag tgcggcgggc caggcgtact gctcgtacga ttgcacgcgc aacttcgcga
      121 acgtccgcgg gacgttgatg gcgaactcgt gcggcggcgc gtattggcac gcgtacaagc
      181 attcaccgca gccgtgacag aggttcgcga ggtagttcaa gtccgccgtc gcaaacgtga
      241 ggcgatcctc catcgcttgg aacgcagggc agtactgctc gcaatagcgg cacgcgttgc
      301 agaccgacag cacccgcgcg ccttcgcgcg tcagctcagt ggcttgcatg acgcgccgcg
      361 ccctcgccgg cgatcctgcc gaataccgtg ccgatcgtca tgccgatgcc tgctatgtag
      421 cctttgcgta acacattccc cgccatgatc tcgccggccg cgtacacgtt cgggaacggc
      481 gcgccgctcg ccgcgagcac gcgcgcgctc gagtcgacgc gcacgccgag gtaagtgaac
      541 gtgatgccgg gccgcagcgg atacgcggca tacggcgggg tatcgaggcg ctgcgcccag
      601 tgcgatttcg cgggagtgag gccggccgtg cggcagtcgt cgagcgtcgt gtgatcgaac
      661 gcgccgggtt gcacggcacg attgaacgcc gcgaccgtcg cttcgaggcg ctcgggcggt
      721 agatcgacct tgcccgcgag ctcgctcaag ctcgcggcct cgatcggcgg atagaccgac
      781 ggcatgaagc ggccgcgcac tttggcgtcg atgatcgagt aggcgttctc gccgggctgg
      841 cgagcgacga gcttgcccca gatcgcgtag cgcttcggcc agaagtcttc gccttcgtcg
      901 tagaaccgtt cggcttgggc gttcacgacg atgccgagcg gcacgcagtc gaggcgcgtc
      961 acgatgccgc cgtcgaactt cggcgcgcgg ccgtcgatgg cgacggcgtg acactcgcgc
     1021 gcgtcgccga tcggcgccgc gccggcgtcg agcatgagct tcagcaccgt gcccgtgttg
     1081 tacggcgtgc cgcggatcag aaaattgcgg gccgcgggac cccaggctcg ctcgagccac
     1141 tcgaggttcg cctcgaagcc tccggccgcg agcacgacag cctgggcgcg cagtgcggtc
     1201 gtcgcgccgc ccgaccgaac cgtcgccgaa tggaacgaac cgtcgcccag cgcgagctcg
     1261 acgacttcgg cgtcgtagcg cacgtccacg ccaagccgct cggccgccgc gtagtagctg
     1321 ttcatgagcg cggtgccgcc gcccaagaag aacgcgttcg tgcgcgcgag ctgcagcgtg
     1381 ccgcgcagcg agctttgaaa gcgagcgccg aggcccgtca tccacggcac gcactcgccc
     1441 gagcgagccg cgacgagcgc cgcgagcgct tcgtcggtct cgccgccgtt gacgcgccgc
     1501 agatcctcga gcagctcctc ttgcgtgtac gtctctgtga ggatctccgt cggcacgtcg
     1561 tgcgcgcagc gcaggttgcg cgtgtggcgg ctgttgccgc cgcgaaacgg ccgcggcgcg
     1621 cactcgagga cgagcacgcg cgcgccgagc cgccgcgccg tcaatgcggc cgtgagaccc
     1681 gcgttgccgc cgccgatcac gagaacgtcg taggccggcg cgtcaggcat gcttgcgccg
     1741 ccggcgtaag tcgagcaacc aaggagcgac gagcagcagc agcgccgcga gcagcaacgc
     1801 cgccgagatc ggccgcgtga cgaacaccgt gacgtcgccg tcgctgatcg ccagcgcgcg
     1861 gcggaactgc tgctcggcca tcggtccgag cacgaggccg agcacggcgg gcacgagcgg
     1921 aaccttgcag aggcgcagca cgaggccgag caagccgacg acgtagagca gggcgacgtc
     1981 gagcagcgag cggttcagcg tgtacgcgcc gagcgacgcg agcacgagca cgccgccgaa
     2041 cagcagcgaa cgcggcagca gcatgactcg cgcccagacg ccggcgagcg gcagattcag
     2101 caccagcagc atcacgttgc cgatgaacag gctcgcgatc agcgtccaaa cgagatcggg
     2161 ccgcgccgag aacagcaatg gtccgggctg caatccatac tgctgaaacg ccgcaagcaa
     2221 gatcgcggcc gtggccgacg acggcacacc gagcgtcaac agcggcacga gcacgccggc
     2281 cgtcgacgcg ttgttcgcag cctccggccc cgcgacgccc tcgatcgcac cgcggccgaa
     2341 ctcttcgggg tgcgcgctca agcgcttttc ggcgaggtag gaaaggaacg tcggaatcac
     2401 ggagccgccc atcggcaaca cgccgagcgg aaagccgatc gcggtgccgc gcagccacgg
     2461 cttccacgat cgcgaccact catcgcgcgt catccacaac gatccccgca acacgacaat
     2521 ctcgggctgt gcaccgagct ggtgacgcga tgccgcatag agcgcctcgc ccacggcgaa
     2581 caggccgacc gccacg
//
LOCUS       DATLEE010000704        10463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8808399, whole genome shotgun sequence.
ACCESSION   DATLEE010000704 DATLEE010000000
VERSION     DATLEE010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8808399"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VM692_15405"
     CDS             complement(<1..185)
                     /locus_tag="VM692_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53613.1"
                     /translation="MKRIERWYLSISAAALFGAGVPAVAHHSDSMFDEKVVELTGTVK
                     ALQWTNPHIWLQVVVEQ"
     gene            complement(190..1026)
                     /locus_tag="VM692_15410"
     CDS             complement(190..1026)
                     /locus_tag="VM692_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53614.1"
                     /translation="MPSLAPAAAFAALTIVAATGVSMHGAAFAQTPVAELPDWSGVWA
                     MEGGTVFDRATVQPLNGRSGEPGVREFPPYTDEWEAIYRRNIELVKQGRFPDPITVCG
                     VPHGFPRLMNLPDVYEFAVTRDAVYVLAENGPNILRIYTDGRSHPGQDELWPTYSGAS
                     VGHWEGDTLVFTTISLKGSSGNDVIVDRTGLVLSDASRATTRLRKVDATTMELQMTIE
                     DAKALKSPWHVTKRYRKQPAGTWAWDYACAENNRNPIANDGRTLTLGPDGKPIDRGTE
                     QR"
     gene            1223..4003
                     /locus_tag="VM692_15415"
     CDS             1223..4003
                     /locus_tag="VM692_15415"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUQ53615.1"
                     /translation="MRLLQAVRVFCISVAALGALTAASQPQATRTRTAAPAAANITRV
                     TSVEGIDEYRLDNGLQVLLFRDPSKATITVNMTYLVGSVDESYGETGMAHLLEHMMFK
                     GSTKYPDILAAIRSRGAQFNGTTSWDRTNYFETFDASDANLEWALDLEADRMVNAFVA
                     QKDLDTEMTVVRNEFESGENNPLRVLLERVQSTSYIWHGYGNSPIGSRSDIENVPIPR
                     LQAFYKRHYQPDNAVLVVAGRFEEQQVLDLVKTKFGTIPRPERELTDDYTIEPTQDGE
                     REVIVRRVGDVQILMASYHVPSGAHPDFVPVQIAAEILADEPAGRLYKALVEPGLASQ
                     IGVQELQLRDPGSITFFALVRQDASLDMARKVMVETIDGLATKPVTAEEVERTRNQAM
                     SGFEQSMNNTQSIAIQLSNWASIGDWRLMFLDRDRVREATVADVQRTALEYFKASNRT
                     VGLFMPGEPDRSDIPARPDVTAMLQGYKGDEARAEGEAFDPTPANIDSRTTRVELANG
                     IKLVMLPKETRGDAVNALIRLNYGDENSLKGVGQFAPLTMQMLMRGSREHSRQEIQDE
                     LARLQSQLNIGGGPGLTSAGIQSTRQNLAAVIELAIEVLREPVFPEDELKTLKDQALA
                     GIESQRSEPQSIVSRAYSRHWARNYARDDVRYVRTVEEDIDFINRVSVADLRNLHSGF
                     LGATGAEVVVVGDFDPQQVRELIEKGLGDWRSPKPFGDVLNLYANLAKDPTAQVFNTP
                     DKENAFFLAGMPIEMRDSHPDYPALVMGNYILGSGPASRLFGRIRGREGLSYGVGSGF
                     GASPRSDAASFNVNAIAAPQNAEKVEASFRDELTTILREGYTEEELAQAKVSWAQQRQ
                     VGRTQDGGLAGTLGLWTHVGRTMAWDADFEAKVQALTVAQVREAMNKHLDLTKMVFMR
                     GGDFEGGGASAQ"
     gene            4503..5624
                     /locus_tag="VM692_15420"
     CDS             4503..5624
                     /locus_tag="VM692_15420"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ53616.1"
                     /translation="MLAKERAYPNLTFTAAVALLRAPNDTSRWFVAQQDGHVVSFADV
                     ANVATTTAVLDISDRVVFRNVHGLLGMAFHPSFPADPRAYVAYTHEPRAGVIVLRVSE
                     FATTDNGSTLSPASEQILFELAQPGGHNNGGHVLFGADGFLYLGIGDGGNDDAASGVA
                     GNGQTTTNLLGKILRLDVGGAAPGLRYRIPTDNPFAGNTPCARDGRGTQDCPEIFAWG
                     FRNPWRWTFDRQGGQLWLGDVGSRDREEVDLVVKGGNYGWRCMEGTRATSLNCGTPPT
                     PLLPPVAEYAHTLGIAVTGGYVYRGTAIPGLAGRYVFADYGSGRIWHIATDTQPTMTM
                     TGTGAFMSGTNPASFAEDADGELFIVDVKSGALYKLIAG"
     gene            5705..7612
                     /locus_tag="VM692_15425"
     CDS             5705..7612
                     /locus_tag="VM692_15425"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ53617.1"
                     /translation="MNRPRSSRQHYDVFRRDYAKGTLDDKIAAADSAATKTQTQIKTR
                     RDYLKDYLRWLWPERWAVGAVFLLAITAAGLDMIEPLFMRFITDNVLLNTAAATPDRL
                     RLLNLAGGAFLAVITLSAGMNIARDYRQRIVNTRVMLTLRRSLFERMLRLPLATLWDM
                     KTGGILSRLSGDVDATTGLLQMAIVSPSISVIRLLIAVTVLFALNWQLAFVALAVIPG
                     AVLISFTSAKRIRPIYRSIRKDVERIDGRVGETFSGIRVVRAFGRELREMLEYARGRN
                     QIVRKELFAHRRELWLWTSWGFALSAVNVAIVWYGGHLYMAGSASIGDIMAFQWYSFL
                     LLNPVWQIVNSFSELQRSLAATERVFEVLASPTDKPDRPDARDAPAVVNEIEFQDVEF
                     EYRAGRPVVRDFNVKVRGGSVVALVGRSGAGKTTVTDLVARFHDPTRGRILLNGVDIR
                     ELKLRTYRDLLALVQQDVFLFDGSVRDNIAYAGHDATDADVEDAARRANAHEFIEHLP
                     ERYDTFIGERGVKLSGGQQQRLAIARAILAAPQILILDEATSNLDTESEQLIQASMAT
                     LLAGRTTFVIAHRLSTIRRANLILLLEDGRIVERGTHTELMARGGIYHGMVVRQATSQ
                     AKDADALALRE"
     gene            7739..8335
                     /locus_tag="VM692_15430"
     CDS             7739..8335
                     /locus_tag="VM692_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53618.1"
                     /translation="MQTKYGLLALAAAGLAASSFAAADERSDQPEPRIVTVGFGAGLN
                     TAQPGNSANHHIIPDTIRVNVGDVVGFTVAGLHYIRVYGNGVKLADVKAQIPDECEVN
                     PLPPATFPPQCGGPPVQVVPEGGLNVYYAGVNPFVSPQPGPPFAQASAATNRVESVSF
                     NAPGRYLAICAVLPHFNDRMYAWIEVRPRGGNQGDNHH"
     gene            complement(8354..8560)
                     /locus_tag="VM692_15435"
     CDS             complement(8354..8560)
                     /locus_tag="VM692_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018947325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 family protein"
                     /protein_id="HUQ53619.1"
                     /translation="MSTRRILGIVLLVVGAALLYFGAQATDAPLEQARERLTGDYSEQ
                     TMLYLICGAAAFIGGLALVVTGKR"
     gene            complement(8550..10088)
                     /locus_tag="VM692_15440"
     CDS             complement(8550..10088)
                     /locus_tag="VM692_15440"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HUQ53620.1"
                     /translation="MKRSLPLLSTAACSAALAIPLLISAPAYAATACADLAKLSLPET
                     KITLAERVPAGDFKAPGVQQPLKVPALCRVTGTVAPAINFEVWLPEGSTWNGRFQAVG
                     GGGLAGIISYPAMAEAVRAGYASSSTDTGHVATDNAWLLDRQRIIDYGYRAIHEMTVK
                     AKAVIENHYGRAAEYSYFNGCSTGGRQGLMTAQRYPDDYDGIASGAPVFRFTNLHMGQ
                     LWTSHATLKKPGAVLTREDLTRVGEAAVAQCDAQDGVKDGIIADPRTCNFDPSKLQGF
                     KAEQIEALKMIYQGAVNPRTGVPIYPGLEPGGEAPQPNNPGWGMIMNGQTPFAIDNAV
                     IGAMGFNNPSWDWKTFDFDRDVSLVDAKLAGTLNAVNPDLSAFKNGGGKLIVWHGWND
                     PGVMPQQTLDYFDSVVEYAGKATGGNGAAYTDDYLRLFMLPGVGHCRGGVGPDQVDWM
                     AALAGWVEKGTKPATITASATRDGKVTMTRPLCPHPQVARYKGQGDTNAAASFECAAP
                     QHEH"
     gene            10242..>10463
                     /locus_tag="VM692_15445"
     CDS             10242..>10463
                     /locus_tag="VM692_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53621.1"
                     /translation="MSTVSKLAAAFVGATALMIATWPGATLAGSLPTGKPEDVGMSSE
                     RLARIGPAMQKSIDAGEIAGVVTLVARRGR"
BASE COUNT         1807 a         3494 c         3433 g         1729 t
ORIGIN      
        1 ttctgctcga cgacgacctg cagccagatg tgcggattcg tccactgcag ggctttgacg
       61 gtacccgtca gctcgacgac tttctcgtcg aacatcgaat cggaatgatg tgcgacggca
      121 ggcacgcctg cgccgaataa cgctgcggca gagatcgaca gataccagcg ttcgatgcgt
      181 ttcatctcgt tacctctgct ccgtcccgcg atcgatcggc ttaccgtccg ggccgagcgt
      241 caacgtgcgg ccatcattcg cgatgggatt gcgattgttt tccgcgcacg cgtagtccca
      301 ggcccacgtg ccagccggct gcttgcggta gcgcttcgtc acgtgccacg gcgacttcaa
      361 cgccttggcg tcctcgatcg tcatctgcag ctccatcgtc gtcgcgtcga ccttgcgcag
      421 ccgcgtcgtc gcgcggctgg catcgctcaa cacgagcccg gtgcggtcga cgatgacgtc
      481 gttgccgctc gagcctttga gactaatcgt cgtgaagacg agcgtgtcgc cctcccagtg
      541 accgacggag gcgccgctgt aggtcggcca gagctcgtcc tggcccggat gcgagcgccc
      601 gtccgtgtag atgcgaagga tgttcgggcc gttctcggcg aggacgtaca cggcgtcgcg
      661 cgtgacggcg aactcgtaga cgtcgggcag gttcatgagc cgcgggaaac cgtgcgggac
      721 gccgcaaacg gtgatggggt cggggaagcg tccttgcttg acgagctcga tgttgcggcg
      781 atagatcgct tcccattcgt cggtgtacgg cgggaactcg cgcacgcccg gctcgccgct
      841 gcgaccgttc agcggctgca cggtcgcacg gtcgaacacg gttccgcctt ccatcgccca
      901 aacgccgctc cagtcgggga gctcggcaac cggggtttgc gcaaatgccg caccgtgcat
      961 cgagacgccg gtggcggcga cgattgtcag cgccgcgaag gccgcggcgg gcgcgaggga
     1021 aggcatgcca ggtccccgaa aaaacagccg ataagcagga taccgcaggt cgccgcaacg
     1081 gccacgatcg gccccgctcc gcaagggcca agcgagcggc gcggctcagc cgagggtacg
     1141 gcggcgggca ccgcaaaaac tccggcgagc cgccgcagtc gaacggcgtg gtaagtttgc
     1201 acaatcacta agagggatgg tcatgagact gctccaggcg gttcgcgttt tctgtatctc
     1261 ggttgccgcg ctcggcgcgc tgacggccgc cagtcaacca caggcgacca ggacaagaac
     1321 ggctgcgccg gcggcggcca acatcacgcg agtcacgtcg gtcgaaggca tcgacgagta
     1381 ccgcctcgac aacggcctgc aagtgttgct gttccgcgat ccctcgaaag ccacgatcac
     1441 ggtcaacatg acgtatctcg tcggctcggt cgacgagagc tacggcgaga ccggcatggc
     1501 gcatctgctc gagcacatga tgttcaaggg ctcgacgaag taccccgaca ttctcgccgc
     1561 catccgcagc cgcggtgcgc aattcaacgg cacgacgtcg tgggatcgca cgaattactt
     1621 cgaaaccttt gacgccagcg acgcgaacct cgaatgggcg ctcgacctcg aagccgaccg
     1681 catggtcaac gcgttcgtcg cgcagaagga cctcgacacc gagatgacgg tcgtgcgcaa
     1741 cgagttcgag tccggcgaga ataaccctct gcgcgtgctg ctcgagcgcg tgcagagcac
     1801 ttcctacatc tggcacgggt acggcaactc gccgatcggc agccgcagcg acatcgagaa
     1861 cgtgccgatc ccgcgcctgc aagcgttcta caagcgccat taccagcccg acaacgccgt
     1921 gctcgtcgtc gcgggccgct tcgaggaaca acaggtcctc gatctcgtga agacgaagtt
     1981 cggcacgatt ccgcgtcccg agcgcgagct gaccgacgac tacacgatcg agccgacaca
     2041 ggatggcgag cgcgaggtca tcgtgcgccg cgtcggcgac gtgcagattc tcatggcgag
     2101 ctatcacgtt ccgtcaggag cgcacccgga cttcgtgccg gtgcaaatcg cggccgagat
     2161 cctcgccgac gagccggccg ggcgcctcta taaggcgctc gtcgagccgg gcctcgcgtc
     2221 gcagatcggc gtgcaagagc tgcagctgcg cgacccgggc agcatcacgt tcttcgcgct
     2281 cgtgcgtcag gacgcgtcgc tcgacatggc gcgcaaggtc atggtggaga ccatcgacgg
     2341 gctcgcgacg aagcccgtga ccgccgagga agtcgagcgc acgcgcaatc aagccatgtc
     2401 cggcttcgag cagtcgatga acaacacgca gtccatcgcg attcagctca gcaactgggc
     2461 gtccatcggc gactggcggc tcatgttctt ggatcgcgat cgcgtccgcg aagcgacggt
     2521 cgccgacgtg cagcgcacgg cgctcgaata cttcaaggcg tcgaaccgca ccgtcggatt
     2581 gttcatgccc ggcgagccgg atcgcagcga cattccggcg cggcccgacg tcacggcgat
     2641 gctgcagggc tacaagggcg acgaggcgcg cgccgaaggc gaagcgttcg acccgactcc
     2701 cgccaacatc gattcgcgca ccacgcgtgt cgagctcgcg aacggcatca agctcgtgat
     2761 gctgcccaag gagacgcgcg gcgatgccgt caatgcgctg atccgcctga attacggcga
     2821 cgagaacagt ttgaaaggcg tcggccagtt cgcgccgttg acgatgcaaa tgctgatgcg
     2881 cggctcgcgc gagcattcgc ggcaggaaat ccaggacgag ctcgcacggc tccagagcca
     2941 gctcaacatc ggcggcggcc cgggcctgac ctccgctggc atccagagca cgcggcagaa
     3001 tctcgcggcg gtgatcgagc tcgcgatcga ggtgctgcgc gagcccgtgt tccccgagga
     3061 cgagctgaag acgttgaagg atcaggcgct cgccggcatc gaatcgcaac gcagcgagcc
     3121 gcagtcgatc gtgtcgcgcg cctatagccg ccactgggcg cgcaactatg cgcgcgacga
     3181 cgtgcgttac gtgcggacgg tcgaggaaga catcgacttc atcaatcgcg tcagcgtggc
     3241 cgatctgcgc aatctgcact cgggcttctt gggcgcgacg ggcgctgagg tcgtcgtcgt
     3301 cggggatttc gatccgcagc aagtccgcga gctcatcgag aaagggctcg gcgattggcg
     3361 cagcccgaag ccgttcggcg acgtcttgaa cctctacgcg aacctcgcga aggatccgac
     3421 ggctcaagtc ttcaatacgc cggataagga gaacgcgttt ttcttggccg gcatgccgat
     3481 cgagatgcgc gactcgcatc ccgattaccc ggcgctcgtg atgggcaatt acatcttggg
     3541 atcggggccc gcgtcgcggc tgttcgggcg catccgcggc cgcgagggct tgagctacgg
     3601 agtcggctcc ggcttcggcg cctcgccgcg cagcgacgct gcgtcgttca acgtcaacgc
     3661 gatcgcggcg ccgcaaaacg ccgagaaagt cgaggcgtcg ttccgcgacg agctcacgac
     3721 catcttgcgc gagggctaca ccgaggaaga gctcgcgcaa gcgaaggtca gctgggccca
     3781 gcagcgccag gtcggccgaa cgcaggacgg cggcttggcc ggcacgctgg gcttgtggac
     3841 gcacgtcggc cgcacgatgg cctgggacgc cgacttcgag gccaaggtcc aagcgttgac
     3901 cgtcgcgcag gtccgcgagg caatgaacaa gcacctcgac ttgacgaaga tggtattcat
     3961 gcgcggtggc gacttcgagg gcggcggcgc ctcggctcaa tgaggcgccg attaggcgtc
     4021 cgacgaagcg ccacatgcgc gtgccgcatg cgcgtgccgt gcccctcggt acgtcgctaa
     4081 tcggtcgccg gcaaaggcct tccgaggcat ggggtagccc agaaccagag cccgaatgac
     4141 ctaaggagac cccgcaggcg ccaaacgacg attaggccaa cagaggcggc gcggtatttg
     4201 caaggctccg gagatcagcg tctcaggggg ccgagttcat gtacggaatc aagcttgtcg
     4261 cagcggcggt ggtgtcgttg ggactgctgg cggcgggatg cagtggggga agcgacgacc
     4321 cggctccaac ggccgccccg gacccgactc cgacgcctgc tcccgccccg acgccgactc
     4381 cgacgcctga tccgactccg acgcctgctc ccaccccggc tccgacgccg gctccgacgc
     4441 ctgatccgcc gccgccgact ccgaccccca ctccgactcc gaccccaacg cctgggccgg
     4501 tcgtgctcgc gaaggagcgt gcgtatccga accttacgtt cacggcagcc gttgcgttgc
     4561 tgcgcgcgcc gaacgacacg agccgctggt tcgttgctca gcaagacggc cacgtcgtgt
     4621 cgttcgccga tgtcgcgaac gtcgcgacga cgacggccgt gctcgacatt agcgatcgcg
     4681 tcgtgttccg caacgtgcat gggctgctcg gcatggcgtt tcatccgagc ttcccggccg
     4741 acccgcgcgc ctacgtggcc tacacgcacg agccgcgcgc cggagtcatc gttttgcgcg
     4801 tgtcggagtt cgcgaccacc gacaacggct cgacgctgag ccccgcgagc gagcaaatct
     4861 tgttcgagct cgcacagccg ggcggccaca acaacggcgg tcacgtgctg ttcggcgcgg
     4921 atggtttcct ttatctcggc atcggcgacg gcggcaacga cgacgccgcc agcggcgtcg
     4981 ccggcaacgg ccagacgacg acgaatctgc tcggcaagat tctgcggctc gatgtcggcg
     5041 gcgcggcgcc ggggcttcgc tatcgaatac cgacggacaa cccgttcgcc ggcaacacgc
     5101 cgtgcgcgcg cgacggcagg ggcacgcaag actgccccga gatcttcgcg tgggggttcc
     5161 gcaatccgtg gcgctggacg ttcgaccgcc aaggaggcca gctgtggctc ggcgacgtcg
     5221 gctcgcgcga ccgcgaagag gtggacctcg tggtcaaagg cggcaactac ggctggcgct
     5281 gcatggaagg cacgcgcgcg acgagcctca attgcggcac gccgccgact ccgctactgc
     5341 cgcctgttgc ggagtacgcc cacacgctcg gtattgccgt gaccggcggc tacgtgtatc
     5401 gcggcactgc gattcccggc ctggcaggcc gctacgtctt cgccgactac ggctccggtc
     5461 gaatctggca catcgcgacc gatacgcagc cgacgatgac gatgacgggc accggcgcat
     5521 tcatgtcggg aacgaacccc gcatcgttcg ccgaggacgc ggacggcgag ctgttcattg
     5581 tcgacgtcaa aagcggcgcg ctctacaagt tgatcgcagg ctgacgctct ggcgcgctgc
     5641 agcgcctgtc acgcacgcgt ttgcgcattg ccgcgccgaa ccgcgcccgc tatcgtcgcc
     5701 gtccatgaat cgtccccgtt cgtcccgcca acactacgac gtcttccgcc gcgactacgc
     5761 gaaggggact ctcgacgaca agatcgccgc tgccgatagc gcggcgacca aaacgcagac
     5821 gcagatcaag actcgccgcg actacctcaa ggactatctg cgctggctct ggcccgagcg
     5881 ctgggccgtc ggcgcagttt tcctgctcgc catcacggcg gcagggctcg acatgatcga
     5941 gccgctcttc atgcgcttca tcacggacaa cgtcttgctg aacacggctg cggcgacgcc
     6001 ggaccgcttg cggctgctga atctcgccgg cggcgctttt cttgccgtca tcacgctgtc
     6061 ggcgggcatg aacatcgcgc gcgactaccg ccaacgaatc gtcaacacgc gcgtgatgct
     6121 gacgctgcgc cgctcgttgt tcgagcggat gctgcgcttg ccgctagcga cgctgtggga
     6181 catgaagacc ggcggaatcc tgtcacgcct ctcgggcgac gtcgacgcga cgacgggcct
     6241 cctacaaatg gcgatcgtgt cgccctcgat ctccgtgatt cgcctcttga tcgccgtgac
     6301 cgtgctgttc gcgctgaatt ggcaactcgc gttcgtcgcg ctggccgtga ttccaggcgc
     6361 cgtgctgatc agcttcacgt cggcgaaacg catccggccg atctatcggt cgatccgcaa
     6421 ggacgtcgag cgcatcgacg gccgcgtcgg cgagacgttc tcgggcattc gtgtcgtgcg
     6481 tgcattcggg cgcgagctgc gcgaaatgct cgagtacgcg cgcgggcgca accagatcgt
     6541 gcgcaaggag ctgttcgcgc atcggcgcga gctctggctt tggacgtcgt ggggctttgc
     6601 gctgtcggcc gtcaacgtcg cgatcgtttg gtacggcggc cacttgtaca tggccggcag
     6661 cgcctcgatc ggcgacatca tggcgttcca gtggtactcg ttcctgctgc tgaatcccgt
     6721 ctggcagatc gtcaactcgt tctccgagct gcaacggtcg ctcgcggcga cggaacgcgt
     6781 gttcgaagtg ctcgctagcc ccaccgacaa gcccgaccgg cccgacgccc gtgacgcgcc
     6841 ggccgtcgtg aacgagatcg agtttcagga cgtcgagttc gagtaccgcg ccggccgccc
     6901 cgtcgtccgc gacttcaacg tcaaggtgcg aggcggatcg gtcgttgcgt tggtcggccg
     6961 cagcggcgcc ggcaaaacga cggtcacgga tctcgtcgcg cgctttcacg acccgacgcg
     7021 cggccgaatc ctgctgaacg gcgtcgacat ccgcgagctg aaattgcgca cgtatcgcga
     7081 tctgctggcg ctcgtgcagc aggacgtgtt cctgttcgac ggctccgtgc gcgacaacat
     7141 cgcctacgcg gggcacgacg cgacggacgc cgacgtcgag gacgcggcgc gccgcgccaa
     7201 cgcgcacgag ttcatcgagc acctgccgga acgctacgac acgttcatcg gcgagcgcgg
     7261 cgtcaagctt tcgggcggcc aacaacagcg gctcgcgatc gcgcgcgcga tcctcgcggc
     7321 gccgcagatc ctgatcctcg acgaggccac gagcaatctc gacacggaga gcgagcaatt
     7381 gattcaagcg tcgatggcga cgctgttggc cgggcgcacg acgttcgtga tcgcgcaccg
     7441 cttgtcgacg atccgccgcg cgaatctcat tttgctgctg gaagacggcc gcatcgtcga
     7501 gcgcggcacg cacaccgagc tcatggcgcg cggcggcatc taccacggca tggtcgtgcg
     7561 ccaagcgacg tcacaagcga aagacgccga cgcgctcgcg ctgcgtgagt aggacgcgcc
     7621 gagcgagtca ccgaagtccc acgaaaggtt ctaggccgcg ggaaaaatat tctcggacgg
     7681 tcgttacgat ccgctcgcta gactggcggt gcgcaccacc acagactggg gagaaaccat
     7741 gcaaaccaaa tacggcctac tcgcgttggc cgccgcgggc ctcgctgctt cgagcttcgc
     7801 ggccgcggac gagcgcagcg atcaaccgga accgcgaatc gtgaccgtcg gcttcggggc
     7861 cggcctcaat accgcgcagc ccggcaactc cgcgaaccat cacatcattc cggacaccat
     7921 ccgcgtcaac gtcggcgatg tcgtcggctt cacggtcgcg gggcttcact acattcgcgt
     7981 ctacggcaac ggcgtgaaac tggccgatgt caaagcgcaa attcccgacg agtgcgaggt
     8041 gaatccgttg ccgccggcta cgttcccacc gcagtgcggt ggaccgcccg tgcaagtcgt
     8101 gcccgagggc ggcctcaacg tctactatgc cggcgtcaat cccttcgtga gcccgcaacc
     8161 ggggccgccg ttcgcgcaag catcggcagc gacgaaccga gtcgagtccg tctcgttcaa
     8221 cgcgccgggg cgctacttag ccatctgcgc ggtgctgccg cacttcaacg accgcatgta
     8281 cgcatggatc gaggtccggc cgcgcggcgg caatcagggc gacaaccacc actgatcgac
     8341 gccggcgcgc aagctagcgc ttgcccgtaa cgaccaaggc gaggccgccg atgaaggcgg
     8401 ccgcgccgca gatcagatac agcatcgttt gctcgctgta gtcgcccgtc agccgctcgc
     8461 gcgcctgctc gagcggcgcg tcggttgctt gtgcgccgaa atacagcagc gcggcgccga
     8521 cgacgagcag cacgatgccg aggatcctcc tagtgctcat gctgcggcgc tgcgcactcg
     8581 aagctagcgg cggcgttcgt gtcgccctgg cccttgtagc gcgcaacttg cgggtgcggg
     8641 cacagcggcc gagtcatcgt gaccttgccg tcgcgcgtcg cggatgccgt aatcgtcgca
     8701 ggcttcgtgc ccttctcgac ccagccggcg agcgccgcca tccaatcgac ttgatcgggg
     8761 ccgacgccgc cgcggcagtg gccgacgccg ggcagcatga agagccgtag gtaatcgtcc
     8821 gtgtacgcgg cgccgttgcc gcccgtggcc ttgccggcgt actcgacgac gctgtcgaag
     8881 tagtcgagcg tctgttgcgg catgacgccg ggatcgttcc acccgtgcca aacgatcagc
     8941 ttgccgccgc cgttcttgaa agcgctgaga tccgggttca cggcgttcaa tgtgccggcg
     9001 agcttggcgt cgacgagcga cacgtcacgg tcgaagtcga acgtcttcca atcccagctc
     9061 gggttgttga agcccatcgc gccgatgacg gcgttgtcga tcgcgaacgg cgtctggccg
     9121 ttcatgatca tgccccaacc cggattgttc ggctgcggcg cctcgccgcc gggctcgagg
     9181 ccgggataga tcggtacgcc cgtgcgcgga ttcacggcac cctgatagat catcttcaac
     9241 gcttcgatct gctcggcctt gaaaccctgc agcttgctcg gatcgaagtt gcaggtgcgc
     9301 ggatcggcga tgatgccgtc cttgacgccg tcctgcgcgt cgcactgcgc gacggccgct
     9361 tcgccgacgc gcgtcagatc ctcgcgcgtc aggacggcgc ccggcttctt cagcgttgca
     9421 tgcgacgtcc aaagctgacc catgtggagg ttcgtgaagc gaaacaccgg cgcgccggag
     9481 gcgatgccgt cgtagtcgtc tggatagcgc tgggccgtca tgaggccttg ccggccgccc
     9541 gtcgagcaac cgttgaagta cgaatactcg gccgcgcgtc catagtggtt ctcgatgacg
     9601 gctttcgcct tgacagtcat ctcgtgaatg gcgcgatagc cgtagtcgat gatgcgttgc
     9661 cgatcgagca accacgcgtt atcggtcgca acgtgccccg tgtccgtcga cgacgaggcg
     9721 tagcccgcgc gcacggcctc ggccatcgcg ggatagctga tgatgcccgc gagaccaccg
     9781 ccgccgacgg cctggaagcg gccattccac gtgctgccct cgggcagcca cacttcgaaa
     9841 ttgatcgccg gcgcgacggt gcccgtcaca cggcacagtg cggggacctt cagcggctgc
     9901 tgcacgcccg gtgccttgaa gtcgccggcc ggcacgcgct cggcgagcgt gatcttcgtc
     9961 tccggaagcg acagcttcgc taggtccgcg cacgcggttg cggcgtaggc cggcgcgctg
    10021 atcagcagcg ggatcgccaa cgcggcggag caagcggcgg ttgaaaggag cggcaacgag
    10081 cgtttcatcg tggtttcccc cggggatgga cggcagagcg ctgtaacgcc aaaaagcata
    10141 ggtggtttgc agcgccgctg tcacgagttt tgccaagagg cagccggcgt cggcgctgcg
    10201 ctgcgctacg atggctcgcc acttatcggg agggggttca catgtcgacg gtgtcgaagt
    10261 tggcggccgc gttcgtcggc gccacagcgc tcatgattgc aacttggccc ggcgccacgc
    10321 tggccggctc gctgcccaca gggaagcccg aggacgtcgg gatgtcgtcc gagcggctcg
    10381 cgcgcatcgg ccctgcgatg caaaagagca tcgatgccgg cgagatcgcg ggcgtggtga
    10441 cgctcgtggc gcgccgcggc cgc
//
LOCUS       DATLEE010000705         2854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11038966, whole genome shotgun sequence.
ACCESSION   DATLEE010000705 DATLEE010000000
VERSION     DATLEE010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11038966"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..429)
                     /locus_tag="VM692_15450"
     CDS             complement(115..429)
                     /locus_tag="VM692_15450"
                     /inference="COORDINATES: protein motif:HMM:NF042751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6665 family protein"
                     /protein_id="HUQ53622.1"
                     /translation="MADRFVDTVAHEIAEEKAGSLGRAGVRLMHALERFRAHEQSTEA
                     ERSHARRERLLWDLVERVESFVVQREACGLRDSRHALAYYGVPKEAVARVGARRPARM
                     SV"
     gene            complement(481..1104)
                     /locus_tag="VM692_15455"
     CDS             complement(481..1104)
                     /locus_tag="VM692_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HUQ53623.1"
                     /translation="MTALDRRSILVLTLCGAIAACSAGSGYEPRNGDIVFQTSRSSQS
                     LAIQLATKSPYSHMGIVYLQDGAPFVFEAVQPVKLTPLQTWIERGETGKFVAKRLRDA
                     DSALTASTLQKMRAAGEALAGKDYDLYFEWSDERIYCSELVWKIFDRGAGIQIGQLAT
                     MADFDLSHPAVQAKVKERFGDRVPADEVVVSPAAMFAADNLVTVYEN"
     gene            complement(1115..1552)
                     /locus_tag="VM692_15460"
     CDS             complement(1115..1552)
                     /locus_tag="VM692_15460"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ53624.1"
                     /translation="MSDTTIRRARPADAAEMARLCGELGYPLSAEEMQRRLGVLLPND
                     RHLVVVAAERDSLYGWAHGEHRFSLEGGDRAELMGLVVDARSRRGGVGRALVAGIEAW
                     VAARGIRSLTVRSNAARAESHPFYAALGYERSKTQHVYTKAFP"
     gene            complement(1549..2520)
                     /locus_tag="VM692_15465"
     CDS             complement(1549..2520)
                     /locus_tag="VM692_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53625.1"
                     /translation="MSRRSEAPQASVIDWLLGSDPSIRWQALRDLTSAPAAEVAAERA
                     RVATEGAGARLLELQARDGPWAGAAWNRGCDSTMHVLTLLREMGLDPESRPAQRALTL
                     VRERVTWQDCGPREVHSNRFFAGEVEPCINGQVAAAGAYFGEDVGGILDRLLAEQLAD
                     GGWNCDAERGSTRSSFNTTICVLEALLEHERNAGATAEVTQARLRGQDYLLERGLLRR
                     KSTGEPIQRDRKGGASWQRFAFPTWWHYDVLRGLDYLRSAGVLPDPRVAEAVALVASK
                     RDEQGRWPLELRHAGKLPIEIDDGEGRPSRWITLRALRVLHWYSRLP"
     gene            complement(2653..>2854)
                     /gene="rimO"
                     /locus_tag="VM692_15470"
     CDS             complement(2653..>2854)
                     /gene="rimO"
                     /locus_tag="VM692_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818484.1"
                     /note="catalyzes the methylthiolation of an aspartic acid
                     residue of the S12 protein of the 30S ribosomal subunit;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HUQ53626.1"
                     /translation="EIDVIVDELDGEQALGRSMADAPEIDGRVYLSRPGSKGASKLKA
                     GTIVTARVTDADEYDLWAEPTA"
BASE COUNT          453 a          991 c          946 g          464 t
ORIGIN      
        1 agatcgcgac gacgagtacg ggatgttcga gtacgcgtgc cacgagggca acctcgcgat
       61 gttcaacatc ctgagcgggc accgcgccga cgagcgcgct gccgcgggac gtcgctagac
      121 cgacatccgc gcagggcggc gggcgccgac gcgggcgacg gcttccttcg gcacgccgta
      181 gtacgccagc gcgtggcgcg aatctctgag gccgcaggcc tcgcgctgca cgacgaacga
      241 ctccacgcgt tcgacgagat cccagagcag ccgctcgcgc ctcgcgtgcg aacgctcggc
      301 ctctgtgctc tgctcgtgcg cgcggaaccg ctccagagcg tgcatgagtc tcacgcctgc
      361 gcggcccagc gagccggcct tttcctcggc gatctcgtgc gcaacggtgt ctacgaagcg
      421 gtccgccatc ctccgagctc ccactcttga ctcgcgatgt ataagagtac ctgccgtcgc
      481 tcagttctcg tagaccgtca cgaggttgtc ggcagcaaac atggcggccg gagagacgac
      541 gacctcgtcg gcgggcacac gatcgccgaa gcgctccttg accttcgctt ggacggccgg
      601 gtgcgagaga tcgaagtcgg ccatcgttgc gagttgaccg atttgaatgc cggcgccgcg
      661 gtcgaaaatc ttccaaacga gctccgagca gtagatgcgc tcgtcggacc actcgaagta
      721 gagatcgtag tctttgccgg ccaacgcctc gcccgcggcg cgcattttct gcaacgtcga
      781 cgcggtcaac gcggagtcgg catcgcgcag gcgtttcgcg acgaatttgc ctgtttcgcc
      841 gcgctcgatc cacgtctgta gcggcgtcag cttgaccggc tgcacggcct cgaagacgaa
      901 cggcgcgccg tcttgcagat agacgatgcc catgtggctg tacggtgact tcgtcgcgag
      961 ctgaatcgcg aggctctgcg acgaccgcga cgtctggaac acgatgtcgc cgttgcgcgg
     1021 ctcgtagccg ctgccggcgg agcaggccgc gattgcgccg cagagggtca acacgagtat
     1081 cgaacgtcga tcgagcgcgg tcatcgcttc tccctcaggg gaaggccttg gtgtaaacgt
     1141 gctgcgtctt gctgcgctcg tagccgagcg ctgcatagaa cgggtgcgac tccgcgcgcg
     1201 cggcgttcga ccggaccgtc agcgagcgaa ttccgcgcgc agccacccaa gcctcgatac
     1261 cggccacgag cgcccggccc acgccgccgc gccgcgatct ggcgtcgacg acgagcccca
     1321 tgagctcggc gcggtctccg ccttcgagcg agaaacggtg ctcaccatgg gcccagccat
     1381 acaacgaatc gcgttcggcg gcgaccacga cgagatgcct gtcgttcggc agcagcacgc
     1441 cgaggcgccg ctgcatttcc tcggccgaca acggatagcc gagctcgccg cagagccgcg
     1501 ccatctcggc ggcgtcggct ggcctggcgc ggcgaatcgt cgtgtcgctc atggcaggcg
     1561 agagtaccag tgcaatacgc gcagcgcgcg caacgtgatc cagcggctcg ggcggccttc
     1621 gccgtcgtcg atctcgatcg gcagcttgcc ggcgtgccga agctcgagcg gccagcggcc
     1681 ctgttcgtcg cgtttcgacg ctacgagcgc gacggcctcg gcgacgcgag ggtcgggcaa
     1741 cacgccggcg ctacgcaggt agtcgaggcc gcgcagcacg tcgtagtgcc accacgtggg
     1801 aaacgcgaaa cgttgccacg atgcgccgcc tttgcgatcg cgctgaatgg gctcgcccgt
     1861 cgacttgcgc cgcaacaagc cgcgctcgag caggtagtct tggccgcgta ggcgcgcctg
     1921 cgtgacctcg gcggtcgcgc cggcgttgcg ctcgtgctcg agcaacgctt ccagcacaca
     1981 gatcgtggtg ttgaaggacg agcgtgtcga gcctcgctcc gcgtcgcagt tccagccacc
     2041 gtcggcgagc tgctcggcca gcaaacgatc gaggatgccg ccgacgtctt cgccaaagta
     2101 cgcgccggcc gcggcgacct ggccgttgat gcagggctcg acctcgcccg cgaagaagcg
     2161 gttcgagtgc acctcgcgcg ggccgcagtc ttgccaagtc acgcgctcgc ggacgagcgt
     2221 aagcgctcgc tgcgctggcc gactctcggg gtcgagaccc atctcacgca gcaacgtgag
     2281 cacgtgcatc gtggagtcgc aaccgcggtt ccacgcggcg ccggcccacg gcccgtcgcg
     2341 tgcttgcagc tccagtagcc gagcgccggc gccctcggtt gcgacgcgcg cgcgctcggc
     2401 ggcgacctcg gccgcgggcg ccgaggtcaa gtcgcgcagc gcctgccagc ggatcgacgg
     2461 atccgagccg agcagccaat cgatgacgct cgcctgcggc gcttcactgc ggcggctcat
     2521 cggacgttca gcaccagcgt ccgcggcgcg ctcagcctgc gcgttcgttc atgcatgaat
     2581 cgcctatcca tgagtttgcg ccgataggcc accatgcgcc ctcgtggtcc gctccagaag
     2641 aagactctcg ttctaggcgg tcggctcggc ccacagatcg tattcgtctg cgtccgtgac
     2701 gcgcgccgtg acgatggtgc cggccttgag cttgctcgcg cctttgctgc ccggcctcga
     2761 caagtaaacg cggccgtcga tctcgggcgc gtcggccatc gagcgcccca aagcttgctc
     2821 gccgtcaagc tcgtcgacga tgacgtcgat ctcg
//
LOCUS       DATLEE010000706         3607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8809280, whole genome shotgun sequence.
ACCESSION   DATLEE010000706 DATLEE010000000
VERSION     DATLEE010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8809280"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VM692_15475"
     CDS             <1..504
                     /locus_tag="VM692_15475"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUQ53627.1"
                     /translation="AAVATLPSQPLTQIYFAPRSRFWEQDGFAPSLFTDTRAGMLAAA
                     RNRDDPTEVTSLTAWITGDNAAALDRVSAADAGRAVLGVIETLRPAARGQLELIGMQS
                     WGADPHAGGAWAYFRPGEVTRFAAVLGHAHGRLKFCGEHLATKSRGMEGAMESAEEAA
                     AAILEQT"
     gene            676..1473
                     /locus_tag="VM692_15480"
     CDS             676..1473
                     /locus_tag="VM692_15480"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011073519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HUQ53628.1"
                     /translation="MNAALQDVTAALAEQSGVEPALNAKAYDEETSREHALEHCNRKF
                     AALKADQRIEWALENLPGNHVLTSSFGAQAAVSLHLVTQVSPNIPVLLVDTGYLFPET
                     YRFIDELTAHMRLNLKVFRASRSPAWQEARHGQRWNQGLEGLEAYNQENKVEPMRRAL
                     RELEVSTWFAGLRRGQAESRKEIPYLEWSGGRWKVHPIAEWTDRDVHRYLTKHGLPYH
                     PLWEKGYVSIGDHHSTKALHEVATLEETRFNGLKRECGIHEINLGDL"
     gene            complement(1504..2010)
                     /locus_tag="VM692_15485"
     CDS             complement(1504..2010)
                     /locus_tag="VM692_15485"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HUQ53629.1"
                     /translation="MSERNPYAPPQTEVRDPPLIREADNLAPRRLRLIGVLVDGLANA
                     VVFGPLLLFSGLWSSLLSDDVPFRVVVLVSLGGVAVFLLLNGYLLATAGQTIGKRVVG
                     TRIVNVADGRIPKFLTLVGGRYGVTWLVSLIPGAGNLYGLVDALFVFRDDRRCLHDLI
                     AGTKVITV"
     gene            complement(2007..3035)
                     /locus_tag="VM692_15490"
     CDS             complement(2007..3035)
                     /locus_tag="VM692_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005494735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HUQ53630.1"
                     /translation="MTLKQLKYLVGIADSGLNITSAAERLFTSQPGISKQLKQLEAEL
                     GVQLFTRKGKSLAAITPAGREVIARARKILREVGNITSLASDLTAELDGTLSIATTHT
                     QARYVLPEVIRAFRERYPRVDLELHQGTSEQIAELISTNKVDFAIATGSQDLFSHLTL
                     MPIYHWHRIVLVPKGHALAKQPKPLELKALADHPLVTYVFSGTGESSFKRAFREHGLE
                     PRVVFTARDADIIKTYVRMGMGVGVIASMAFECTDREDLVAIDASELFPRVTTWLGFP
                     RDMVLRGYMVDFVHLFAPHYSPRLIRDAARAQSQVEVDELLREIALPLKGGCEQEQQL
                     PARARAAD"
     gene            3230..>3607
                     /locus_tag="VM692_15495"
     CDS             3230..>3607
                     /locus_tag="VM692_15495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HUQ53631.1"
                     /translation="MQHLPLFADLKNRAVVVVGGGIVAERRVNLLLDAGAAVTVVAPT
                     LTERLTELETEGRFTHVARTYNGDSLEPFWLVIAATDDRAVNAAVAAAALAAKRFCNV
                     VDDLELCTFIMPAIVDRSPVTIAI"
BASE COUNT          661 a         1187 c         1156 g          603 t
ORIGIN      
        1 gcagccgtcg cgacattgcc ctcgcaaccg ctgacgcaaa tttatttcgc gccgcgcagc
       61 cgcttttggg agcaagacgg ttttgcgccg agcttgttca ccgacacgcg cgccggcatg
      121 ctggccgccg cgcgcaatcg cgacgacccg accgaggtga cgagcctcac ggcctggatc
      181 accggcgaca atgccgcagc gctcgatcga gtgtcggcgg ccgacgcggg gcgcgccgtg
      241 ctcggtgtca tcgaaacgct acggccggcg gcgcgcggcc agctcgagct gatcggcatg
      301 cagtcgtggg gcgccgaccc tcacgcaggc ggcgcctggg cttacttccg ccccggcgag
      361 gtcacgcggt ttgccgccgt gctcgggcat gcgcacgggc ggctcaagtt ctgcggcgag
      421 cacctcgcca ccaaaagccg cggtatggaa ggcgccatgg aatcggccga ggaggccgcc
      481 gcggcaattc tcgagcagac ctgaacccgt acccgcgttg ggtttccaag ccccctatgc
      541 cttattcgtt tggagcatag cggtagaaga acttaaccgt tcacggacat aagggcaggc
      601 ccttaccttt agccctaaat acctacgcca tagcggcagc ttccgcctcc gcagttggct
      661 tttggggccc gccctatgaa tgcagcactt caagacgtca ccgcagcgct cgccgagcag
      721 tcgggcgtcg aaccggcgct caacgccaag gcgtacgacg aggaaacgag ccgcgagcat
      781 gcgctcgagc attgcaaccg gaagttcgct gcgctgaagg ccgaccaacg catcgagtgg
      841 gcgctggaga atctacccgg caatcacgtc ctgacctcga gcttcggcgc gcaagccgcc
      901 gtctcgttgc acctcgtcac gcaagtcagc ccgaacattc ccgttctctt agtggacacg
      961 ggctacctgt tccccgaaac gtatcgcttc atcgacgagc tgacggcgca catgagactg
     1021 aacctcaaag tgttccgcgc ctcccgctcg ccggcatggc aggaagcgcg ccacggccaa
     1081 cgttggaacc aagggctcga aggcctcgaa gcgtacaacc aagagaacaa ggtcgagccg
     1141 atgcgccgcg cgttgcgcga gctcgaggtc agcacttggt tcgcgggatt gcgccgcggc
     1201 caagccgaga gccgcaagga aatcccgtat ctcgagtggt ccggcggccg ttggaaagtg
     1261 catccgatcg ccgagtggac cgaccgcgac gtgcaccgtt acctgacgaa gcacggcctg
     1321 ccctaccacc cgctctggga aaaaggctac gtgtcgatcg gcgaccacca cagcaccaag
     1381 gcgctgcacg aggtcgcgac gctcgaggag acgcgcttca acggcttgaa gcgcgagtgc
     1441 ggcatccacg agatcaacct cggcgacctc tagccccgcg catccgtcgc actttcctgc
     1501 atctcagacc gtgatgacct tcgtcccggc gatgaggtcg tgcagacacc gccggtcgtc
     1561 gcgaaagacg aataacgcat cgacgagccc gtacaagttg cccgcgccgg ggatcaagct
     1621 gacgagccaa gtgacgccgt agcgtccgcc gacgagcgtc agaaacttgg ggatgcggcc
     1681 gtcggcaaca ttcacgatac gcgtgccgac cacacgtttg ccgatcgtct gccccgcggt
     1741 cgccagcagg tagccgttga gcagcaggaa cacggcgacg ccgccgagag agacgaggac
     1801 gaccacgcga aaggggacgt cgtcggacag cagcgatgac cataatccgg agaaaagcag
     1861 caatggcccg aatacgaccg cgttggcaag cccgtcgaca agcacgccga tgagccgcaa
     1921 tctgcgcggc gcgagattgt ccgcttcgcg aatcagcggc ggatcgcgaa cctccgtctg
     1981 cggcggtgcg taaggattgc gctcgctcaa tccgcggctc gagctcgcgc ggggagctgt
     2041 tgctcctgct cgcagccgcc tttgagaggc agcgcgatct cgcgcagcaa ctcgtcgacc
     2101 tcgacctgcg actgcgcccg ggccgcgtcg cgaatcagcc gcggcgaata gtgcggcgcg
     2161 aacaggtgca cgaagtcgac catgtagccg cgcagcacca tgtcgcgcgg gaaaccgagc
     2221 cacgtcgtca cgcgcgggaa aagctcgctt gcgtcgatcg cgacgaggtc ctcgcgatcc
     2281 gtgcactcga acgccatcga ggcgatgact ccgacgccca tccccatgcg cacgtaggtt
     2341 ttgatgatgt cggcgtcgcg cgccgtgaag acgacgcgcg gctcgagccc gtgctcgcgg
     2401 aacgcccgct tgaacgacga ctcgccggtg ccgctgaaga cgtacgtgac gagcgggtga
     2461 tcggcgagcg ctttcaactc gagcggcttc ggctgcttcg cgagcgcgtg gcctttcggc
     2521 acgagcacga tgcgatgcca gtggtagatc ggcatcagcg tcaggtgcga gaacaggtct
     2581 tgtgagccgg ttgcgatcgc gaagtcgact ttgttggtgc tgatgagttc ggcgatctgc
     2641 tccgacgtgc cttggtgcag ctcgaggtcg acgcgcgggt aacgctcgcg aaacgcgcgg
     2701 atgacctcgg gcaggacgta gcgcgcctgc gtgtgcgtcg tcgcgatcga caaggtgccg
     2761 tcgagctcgg ccgtcagatc gctggcgagg ctcgtgatat tgccgacctc acgcaggatc
     2821 ttgcgtgccc gggcgatgac ttcgcggccg gccggcgtga tcgcggccaa actcttgccc
     2881 ttgcgcgtga acagctgcac gccgagctcg gcctcgagct gcttcaactg tttgctgatg
     2941 ccgggctggc tcgtgaagag ccgttcggcc gcggacgtga tgttcaagcc gctgtcggcg
     3001 atgccgacga ggtacttgag ctgcttcaac gtcacgcgcg acgaacccct tcagatgaaa
     3061 aggccgcgtc ctcattcgag tcggcggcca cctggggtca tataaccatt ggttagatcg
     3121 aggtgtcaac gacgcccccg ttagctcgcg aagctataaa cctaggcgaa cacgttgttg
     3181 tttggcggcc tacgggcgcg ctatcgtgca gaaactaggc cgttttccca tgcagcacct
     3241 acccctgttc gcggatctga agaatcgcgc cgtcgtcgtc gtcggcggcg ggatcgtggc
     3301 cgagcgccgg gtgaatctgt tgctggatgc cggcgcggcc gtgaccgtcg tcgcccctac
     3361 gcttacggag cgattgaccg agctcgagac ggagggccgc ttcacgcacg tcgcacgcac
     3421 ttacaacggc gactcgctcg agccgttttg gctcgtcatc gcggccaccg acgatcgcgc
     3481 cgtcaatgcg gccgtcgccg ccgcggcgct cgctgcgaaa cgcttctgca atgtcgtcga
     3541 cgatctcgag ctctgcacgt tcatcatgcc ggccatcgtc gaccgctcgc cggtcacgat
     3601 cgcgatc
//
LOCUS       DATLEE010000707         7316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6447264, whole genome shotgun sequence.
ACCESSION   DATLEE010000707 DATLEE010000000
VERSION     DATLEE010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6447264"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2742)
                     /gene="treS"
                     /locus_tag="VM692_15500"
     CDS             complement(<1..2742)
                     /gene="treS"
                     /locus_tag="VM692_15500"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HUQ53632.1"
                     /translation="MADHSADDPLWYKDAVIYQLHVRSFFDSNDDGVGDFAGLTSKLD
                     YIARLGVSAIWLLPFYPSPMRDDGYDIADYLGINPVYGTAREFQTFVREAHARGLKII
                     TELVVNHTSDQHPWFQAARTAPRGSAKRELYVWSDTDKRFAETRIIFTDTEQSNWTWD
                     PVAEQYYWHRFFSHQPDLNHNNPTVVNGLIQVMQYWLDLGVDGLRLDAIPYLCVKDGT
                     NNENLPETHAVVKRFRRAIDAKYKNRMLLAEANQWPEDVREYFGDGDECHMAYHFPLM
                     PRMYMAIAQEDRYPIVEILQQTPDIPPTCQWAIFLRNHDELTLEMVTDRERDYMYQMY
                     AADPRARVNVGIRRRLAPLMENDPAKIQLMNSLLFSMPGSPTVYYGDELGMGDNIYLG
                     DRNSVRTPMQWSPDRNGGFSRADPQSLYLPPIQDPIYGFASVNVEAQSREPSSLLNWT
                     RRILAVRKSYKAFGRGTFELLRPGNRTILAYLRRYENQAILCVANLKRSAQAVELNLS
                     AFRGRVPVELVGRSSFPPIGELPYMLTLPGYAFYWFELAETAPPPSWHEERLPAEERP
                     WLVLFNGLDSFHAGTGDERQSAAAKLHVQLEREVVPSYLATQRWFAGKGQGEPTVAIT
                     DLGRWQPNKELWLLTVVSAQFPADTAQEYFLPLALEWGWERAGPHAAHALARVRQRAA
                     TGLVYDAFADEHFVRSLIGALAERSSVRIGAATLRFTPTNALGALLPKRLAQTPVRQT
                     AESSNLTFVIGEQLFLKAYRRLRRGAHPEWELGRFLTERSPCPAVAATAGAIVLERPG
                     AEPTTVALVQAAVQNQGDGWRYTLRYLERSIDDAVARAADGAVALADHSSYLLLVQAL
                     AARTADLHRALAVPTGDPSFDPVPFAAEQLAEWARAIHAELDATLFTLRARRSELAVD
                     "
     gene            complement(2766..4754)
                     /locus_tag="VM692_15505"
     CDS             complement(2766..4754)
                     /locus_tag="VM692_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HUQ53633.1"
                     /translation="MEWPEDGRRRAFIEQLRPVVDCGRAAIKRVLGEAIEVEADVLVE
                     GHEQIACALLHAPPGSSAWTTVPMTPLGDDCWTATFTPATLGRHRYTVAAWLDAFATW
                     RSKLIRRIARDDIELALREGAVLVRAAAGRARGAERGALLAYAAMLESEAPIDERRRS
                     ALTEALRELMTAAPDRALESVYDPPLEAVVERPLARFSAWYEFFPRSAVDAGQRHGTL
                     ATAARRLGYVAEMGFDVVYLPPIHPIGTSHRKGPNNRLQAAEADPGSPWAIGGAGGGH
                     KSVHAELGTLDDFKAFRSEAERLGLEVALDLAFQCSPDHPYVAEHPLWFARRPDGSVQ
                     YAENPPKKYEDIYPLDFGSAEWRGLWQELNDVVEFWIAAGVRVFRVDNPHTKPFDFWR
                     WLIADVQSRHPDTLFLSEAFTRPRVMFRLAKLGFSQSYTYFTWRNSKQELTDYFTELK
                     FRLDYFRPNLWPNTPDILHEHLQTGGRPAFMARAALAATLGASYGIYGPAFELLEHEP
                     REAGSEEYLDSEKYEVRVWDLERAGSLRDFLARLNAIRRANPALHSDATLRFLTIDND
                     QLICYSKHGDDGANVILVVVNLDPQHAQSGFIDVPLEEWGLDAQNPYGAHELLTDSRH
                     EWQGARVFVTLAPAECPACVYRIEARRSTSEHDFDYFI"
     gene            5009..5620
                     /locus_tag="VM692_15510"
     CDS             5009..5620
                     /locus_tag="VM692_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HUQ53634.1"
                     /translation="MAKARTAKKGRRSKTTSRRRSRSASNGSNRDDAIGLLKSDHREV
                     EQLFKQFEDSRGEDRKQEIAAQICEALKAHTMIEEEIFYPAFLEATDETDIHHEAEVE
                     HQGAKHLIEEIESAGPSDDHFDARVTVLQEMIRHHVNEEEQRGGMFSKAQSSEMDLTE
                     LGRQMRERKLEIMGESAEMGGSRRPPQQGRSRRGGRELRARSS"
     gene            complement(5651..>7316)
                     /gene="treZ"
                     /locus_tag="VM692_15515"
     CDS             complement(5651..>7316)
                     /gene="treZ"
                     /locus_tag="VM692_15515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HUQ53635.1"
                     /translation="QRATDGFFYGFDPDAGAGTRYAFRFDTTDLVVPDPASRFNPDGV
                     HAASQVVDAEAFDWPDDGWRGRPWTEAVIYELHVGTFTPEGTYAAAIERLDYLADLGI
                     TAIELMPLAATPGRWNWGYDGVLPYAPHAGYGRPEDLKRFVAAAHARNLMVLLDVVYN
                     HFGPDGNYLGCYAPEFFTAKHKTPWGNALNYDDTASRVVRQFFIHNALYWLEEYRFDG
                     LRLDAVHTILDDSQPSFLRELAATVHALDLKRPLHLVLENDANDAALLERDAARRPRA
                     YTAQWNDDFHHALYVLLTGEARGHYADYAQPCAQLLRTLLEGFAYQGEQSAYRGAKRG
                     SSSAALPPEAFVNFLQNHDQIGNRPDAARMWRLLEGRRQLAAETLLALLPTPIMLFMG
                     DELHAPSGFPFFCDFTGELGRAVTEGRHAEFATLWRDIDAAAVPGPTTEAARLAAVLD
                     WSALEKEPHRGAFERARQQLAVRRRELHPRLPARAAAGKVLGPCSLLASWTLADGTTL
                     ELAANLAATPCLAPATRAGRRLLATSDSAAAREWPPWHVEWQLLEP"
BASE COUNT         1207 a         2340 c         2592 g         1177 t
ORIGIN      
        1 gtccacggcg agctcgctgc gccgcgcacg cagcgtaaac agcgtagcgt cgagctccgc
       61 atggatcgct cgcgcccatt cggcgagctg ctcggctgcg aacggcacgg ggtcgaagct
      121 cggatcaccg gtcggcaccg cgagcgcgcg gtgcagatcg gccgtgcgcg cggcgagcgc
      181 ctgcacgagc agcagatagc tcgagtggtc cgccaacgcg acggcgccgt cggccgctcg
      241 cgcgacggca tcgtcgatgc tgcgctcgag gtagcgcaac gtgtagcgcc agccgtcgcc
      301 ctggttttgc acggcggcct gcacgagcgc gacggtcgtc ggctcggccc cgggccgctc
      361 gagcactatg gctccggccg tggccgcgac ggccgggcag ggcgagcgct ccgtgaggaa
      421 tcggccgagc tcccactccg ggtgcgcgcc gcggcgcagc cggcgatagg ctttcagaaa
      481 cagctgctcg ccgatgacga aggtcaggtt gctgctctcg gcggtctgcc gcacgggcgt
      541 ttgcgcgagc cgcttcggta gcagcgcgcc gagcgcgttc gtcggcgtga agcgcagcgt
      601 cgcggcgccg atgcgcaccg acgagcgctc cgccagcgcg ccaatcaggg agcgcacgaa
      661 gtgctcgtcg gcgaacgcgt cgtacacgag ccccgtggcg gctcgctgcc gcactcgcgc
      721 aagcgcgtgc gcagcgtgcg ggcccgcgcg ctcccagccc cactcgagcg cgagcggcag
      781 gaaatattcc tgcgcagtgt ccgcggggaa ttgcgccgag acgaccgtca gcagccagag
      841 ctccttgttc ggctgccagc ggccgaggtc cgtgatggcg accgtcggct cgccttggcc
      901 tttgccggcg aaccagcgct gcgtcgcgag atagctcggc acgacttcac gctcgagctg
      961 tacgtgcaat ttggcggcgg cgctctgccg ctcgtcgccc gtgccggcgt ggaagctgtc
     1021 gaggccgttg aacagcacga gccagggccg ctcctcggca ggcaagcgtt cctcgtgcca
     1081 gctcggcggc ggtgcggtct cggcgagctc gaaccagtag aacgcgtagc ccggcaacgt
     1141 cagcatgtag ggcagctcgc cgatcggcgg gaacgacgac cggccgacga gctcgacggg
     1201 cacgcggccg cgaaaggccg agagattcag ctccacggcc tgcgcggagc gcttgaggtt
     1261 ggcgacgcag aggatcgctt ggttttcgta gcggcgcagg tacgcgagga tcgtgcgatt
     1321 gccgggccgc agcagctcga aggtcccgcg gccgaacgcc ttgtagctct tgcgcacggc
     1381 gaggatgcgc cgcgtccagt tcaacagcga cgacggctcc cgcgattgcg cctcgacgtt
     1441 gacggacgcg aagccgtaga tcgggtcctg gatcggcggc aagtacaggc tctgcggatc
     1501 cgcgcgcgag aagccgccgt tacggtcggg gctccactgc atcggcgtgc ggacgctgtt
     1561 gcgatcgccg agatagatgt tgtcgcccat gccgagctcg tcgccgtagt agaccgtcgg
     1621 cgagcccggc atcgagaaca gcaagctgtt catgagctgg atcttcgcgg ggtcgttctc
     1681 catcagcggc gcgagccggc ggcgaatgcc gacgttgacg cgagcgcgcg ggtccgccgc
     1741 gtacatctgg tacatgtaat cgcgctcacg gtccgtgacc atctcgagcg tcagctcgtc
     1801 gtggttacgc aggaagatcg cccattggca cgtcggcgga atgtccggcg tctgctgcag
     1861 gatttcgacg attgggtaac ggtcttcctg cgcgatcgcc atgtacatgc gcggcatcag
     1921 cgggaagtga taggccatgt ggcactcgtc gccgtcaccg aaatactcgc gcacgtcctc
     1981 gggccactga ttcgcttcgg cgagcagcat gcggtttttg tacttggcgt cgatcgcgcg
     2041 gcgaaaacgc ttcacgacgg cgtgtgtctc gggcaggttc tcgttgttcg tgccgtcctt
     2101 cacgcagagg tacgggatcg cgtcgagccg caggccgtcg acgcccaagt cgagccagta
     2161 ctgcatgacc tgaatcaggc cgttcacgac cgtcgggttg ttgtggttca ggtcgggctg
     2221 atgcgagaag aatcgatgcc agtagtactg ctcggcgacg gggtcccaag tccagttcga
     2281 ctgctcggtg tccgtgaaga tgatgcgcgt ctccgcgaaa cgcttgtcgg tgtcgctcca
     2341 aacgtacagc tcgcgcttcg ccgagccgcg cggcgccgtt cgtgccgcct gaaaccatgg
     2401 atgctggtcc gaggtgtggt tgacgacgag ctccgtgatg atcttgagcc cgcgcgcgtg
     2461 cgcctcgcgc acgaacgtct ggaactcgcg cgcggtcccg taaacggggt tgatgccgag
     2521 atagtcggca atgtcgtagc cgtcgtcgcg catcggcgac gggtagaacg gcagcagcca
     2581 aatggcgctg acgccgagcc gtgcgatgta gtcgagcttc gacgtgagcc cggcgaagtc
     2641 cccgacgccg tcgtcgttcg aatcgaagaa cgagcggacg tggagttggt agatgacggc
     2701 gtctttgtac cagagcggat cgtcggctga gtggtcggcc atggaggctc gggccctttc
     2761 tgtcgctaga tgaagtagtc gaagtcgtgc tcggacgtgc tgcgtcgcgc ttcgatgcga
     2821 tagacgcacg ccggacattc ggcgggcgcg agcgtcacga acacgcgggc gccttgccat
     2881 tcgtggcgcg agtcggtcag gagctcatgc gcgccatacg ggttttgcgc gtcgaggccc
     2941 cattcttcga gcggcacgtc gatgaacccg ctctgtgcgt gctgcggatc gagattgacg
     3001 acgacgagaa tgacgttcgc gccgtcgtcg ccgtgcttcg agtagcagat gagctgatcg
     3061 ttatcgatgg taaggaaccg cagcgtcgcg tcgctgtgca gcgccggatt ggcgcgccga
     3121 atcgcgttca agcgcgcgag aaagtcgcgc agcgagcccg cgcgttcgag atcccagacg
     3181 cgcacctcgt acttctcgga gtcgaggtac tcctcgctgc cggcctcgcg cggctcgtgc
     3241 tcgagcagct cgaacgcggg gccgtagatg ccatagctgg caccgagcgt ggcggcgagc
     3301 gcggcgcgcg ccatgaatgc cggcctgccc cctgtctgta ggtgctcgtg gaggatgtcc
     3361 ggggtgttcg gccagaggtt cgggcgaaaa tagtcgagcc taaacttcag ctccgtgaag
     3421 tagtccgtga gctcttgctt gctgttgcgc caggtgaagt acgtgtacga ctggctgaag
     3481 ccgagcttcg cgagccgaaa catcacgcgt ggccgcgtga acgcttcgga aagaaacagc
     3541 gtgtcgggat gccggctctg gacgtcggcg atcagccagc gccagaagtc gaacggcttc
     3601 gtgtgcggat tgtcgacgcg aaagacccgc acgccggccg cgatccagaa ctcgacgacg
     3661 tcattgagct cttgccagag cccgcgccac tcggcgctgc cgaagtcgag cgggtagatg
     3721 tcctcgtact ttttcggcgg gttttcggcg tactgcacgc tgccgtcggg gcggcgcgcg
     3781 aaccagagcg gatgctcggc gacgtacggg tgatccggcg agcactggaa cgcgagatcg
     3841 agcgccacct cgaggccgag ccgctcggct tcgctgcgga aagccttgaa gtcgtcgagc
     3901 gtcccgagct cggcgtgcac gctcttgtgg ccgccgccgg ccccgccgat ggcccacggc
     3961 gatccgggat cggcttcggc ggcttgcagg cgattgttcg ggcctttgcg gtggctcgtg
     4021 ccgatcggat gaatcggcgg cagatagacg acgtcgaagc ccatttcggc gacgtagccg
     4081 agacggcggg ccgccgtcgc caatgtgccg tgccgctggc ccgcgtcgac ggccgagcgc
     4141 gggaagaact cgtaccacgc ggagaaacgc gcgagcgggc gctcgacgac ggcttcgagc
     4201 ggcggatcgt agacgctctc gagcgcacgg tccggcgctg ccgtcatgag ctcgcgcagt
     4261 gcttcggtca gtgcgctgcg gcggcgctcg tcgatcggcg cctcgctctc gagcattgcg
     4321 gcataggcga gcagtgcgcc gcgctcggcg ccacgcgcgc ggcccgcggc ggcccgcacg
     4381 agcaccgcgc cttcgcgcag tgcgagctcg atgtcgtcgc gtgcaatccg gcgtatcagc
     4441 ttcgaccgcc acgtcgcgaa cgcgtcgagc caggccgcaa ccgtgtagcg atggcggccc
     4501 agggttgcgg gggtgaaggt ggcggtccag cagtcgtcgc cgagcggcgt catcggcacg
     4561 gtcgtccatg ccgagctgcc gggcggcgca tgcagcagcg cgcacgcgat ttgctcgtgc
     4621 ccttcgacga gcacgtcggc ctcgacttcg atcgcttcgc cgagcacgcg cttgatggct
     4681 gcacggccgc agtcgaccac gggacgcagc tgttcgatga atgcccggcg gcgcccgtcc
     4741 tctggccatt ccatatgagt tcgacgacaa cgttgtctag gcaagacgga gcaagttcta
     4801 tgccgcttcg cagcgcgtcg cccgcggagg cgaaagacgc gcgacgctcg cggcgcgcgc
     4861 ggacctcgca atagttgcaa cctactccca aaaggtgtgg tgcaaacgac acggtctagt
     4921 ggttcgttga gcagcggttc acgtgcggtt cgtggcacga gacgtgcagc caccaggcgc
     4981 ttcatccacc gacggaagag ggaaggccat ggccaaagca cgaactgcga agaagggacg
     5041 ccgctcgaag acgacgtcac gacggcgctc gcgatcggcg agcaacggca gcaatcgcga
     5101 cgacgcgatc ggcttgctga aatccgacca ccgcgaggtc gaacagctgt tcaagcagtt
     5161 cgaggactcg cgcggagagg atcgcaagca ggagatcgcg gcgcagatct gcgaggcgct
     5221 taaagcgcac acgatgatcg aggaagaaat cttttatccg gcgttcctcg aggcgacgga
     5281 cgagacggat atccatcacg aggccgaggt ggagcaccag ggtgcgaagc acctgatcga
     5341 ggaaatcgag tcggccgggc cctccgacga ccacttcgac gctcgcgtga cggtgctgca
     5401 ggagatgatc cggcaccacg tcaacgagga agagcagcgc ggcggaatgt tcagcaaggc
     5461 ccagagcagc gagatggatt tgaccgagct cggccggcag atgcgcgagc gcaagctcga
     5521 gatcatgggt gaatcggccg agatgggcgg aagccgccgc ccgccgcagc aaggccggtc
     5581 gcgccgtggc ggccgcgagc tccgcgcccg ctcgagctga ttgcgagcgt cggcagccgc
     5641 ggggcgtagg tcaaggctcg agcagctgcc actcgacatg ccacggcggc cactctcgcg
     5701 cggcggccga gtccgacgtc gcaagtaggc gtcggccggc gcgcgtggcc ggcgccaaac
     5761 acggcgtagc cgcgagattt gcggctagct cgagcgtggt gccgtcggcc aaggtccagc
     5821 tggccagcag cgagcacggc ccgagcactt ttccggccgc ggcgcgcgcg ggcaagcgcg
     5881 ggtgcagctc ccggcgacgg accgccaatt gctgtcgcgc tcgctcgaac gcgccgcggt
     5941 gcggttcttt ctcgagcgcc gaccaatcga ggacggccgc gagccgcgcg gcttcggtcg
     6001 tcgggcccgg gacggccgcc gcgtcgatat cgcgccacaa cgtcgcaaac tcggcgtgcc
     6061 ggccttcggt cacggcgcgg cctagctcgc ccgtgaaatc acagaagaac gggaagccgc
     6121 tcggcgcgtg cagctcgtcg cccatgaaca gcatgatcgg cgtcggcagt agcgcgagca
     6181 gcgtctcggc cgcaagctga cggcggcctt cgagcagccg ccacatacgc gccgcgtcgg
     6241 ggcgattgcc gatctgatcg tggttctgca gaaaattcac gaaagcctcg ggtggcagcg
     6301 cggcgctcga gctgccgcgc ttcgcgccgc gataggccga ctgctcgcct tggtaagcaa
     6361 agccctcgag cagcgtgcgc agcagctgcg cgcagggctg cgcgtagtcg gcgtagtgcc
     6421 cgcgcgcctc gcccgtcaac agaacgtata gcgcgtgatg aaagtcgtcg ttccattgcg
     6481 ccgtgtaggc gcggggccgg cgcgccgcgt cgcgctcgag caatgcggcg tcgttggcat
     6541 cgttctcgag cacgaggtgc aacggccgct tgagatcgag cgcgtgcacg gtggccgcga
     6601 gctcgcggag gaagctcggc tgcgagtcgt cgaggatcgt gtgcacggca tcgagccgca
     6661 gcccgtcgaa acgatactcc tcgagccagt acagagcgtt gtgaatgaag aactgacgca
     6721 ccacgcgcga cgccgtatcg tcgtagttca gggcgttgcc ccatggcgtc ttgtgcttcg
     6781 ccgtgaagaa ctcgggtgcg tagcagccga gatagttgcc gtcgggcccg aagtggttgt
     6841 agacgacgtc gagcaggacc atgaggttgc gcgcgtgcgc ggcagcgacg aaccgcttga
     6901 gatcctcggg ccgtccatag ccggcgtgcg gcgcgtacgg cagcacgccg tcgtagcccc
     6961 agttccacct tcccggcgtc gccgccagcg gcatcagctc gatagccgtg atcccgagat
     7021 cggcgaggta gtcgagccgc tcgatcgcgg cagcataggt tccttcgggc gtgaaagtgc
     7081 cgacgtgcag ctcgtagatg acggcttcgg tccacggccg gccgcgccag ccgtcgtcgg
     7141 gccaatcgaa tgcctcggcg tcgacgactt gggacgccgc gtgcacgccg tcggggttga
     7201 agcgcgaggc cgggtccggt acgacgaggt ccgtcgtgtc gaaccgaaac gcatagcgcg
     7261 taccggcgcc cgcgtcggga tcgaacccgt agaagaagcc gtcggttgcg cgctgc
//
LOCUS       DATLEE010000708         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3608363, whole genome shotgun sequence.
ACCESSION   DATLEE010000708 DATLEE010000000
VERSION     DATLEE010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3608363"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VM692_15520"
     CDS             complement(<1..530)
                     /locus_tag="VM692_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53636.1"
                     /translation="MKAVIALVLACFTPALGVAATLTGSIVNSSTAVDLPKVGTLDWA
                     RWPGYTHKGTAISDVTTTGTLKSMSNDPRLIGDNTAIKVAGSNASFQFTVAATTVERT
                     LTYYIGGWNSTGRVTVTMAGASTYTTTFSSTTTYSRVVTVKYRADAPTTLKVTYTQTA
                     GAGSINMQAAALSTLG"
     gene            694..1002
                     /locus_tag="VM692_15525"
     CDS             694..1002
                     /locus_tag="VM692_15525"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HUQ53637.1"
                     /translation="MLQSIADLKRYAVYNDRGNKLGVIDDVILDADEWTVCYAVLRYE
                     VAGEPTKLLGVGLDALTLDSENQCLVVAADEALLSRASGFDRNAPPAKPEPLFNSPDH
                     "
     gene            complement(999..1166)
                     /locus_tag="VM692_15530"
     CDS             complement(999..1166)
                     /locus_tag="VM692_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53638.1"
                     /translation="MGGIGDAVSYTVLALGFVVLIFVGALFFVQASRRGNRRFKWWRG
                     RRTEDETTVER"
     gene            complement(1171..1515)
                     /locus_tag="VM692_15535"
     CDS             complement(1171..1515)
                     /locus_tag="VM692_15535"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HUQ53639.1"
                     /translation="MRLISANTLSAADVRSAAGETLGKIVDFMLDTERGSVVYAVLSV
                     GGVLGVGAKMLAIPPESLGYDSGQRALSVGIDTAALDEATGIDRANPPDHADGDLSRS
                     QIAHSLSSRNQR"
     gene            1712..>2087
                     /locus_tag="VM692_15540"
     CDS             1712..>2087
                     /locus_tag="VM692_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HUQ53640.1"
                     /translation="MRNDIVAWLPSIAAACGVSVLAGCGHSASLTVAEGMGPRPELPA
                     PDRSLIPVIKIAPATGWQNGGAPVAAAGLSVQPFARDLDHPRWLYVLPNGDVLVAETN
                     APERPEDRKGIKGAIMALAMKKA"
BASE COUNT          366 a          676 c          684 g          361 t
ORIGIN      
        1 ctgcccaacg tcgacagcgc tgcggcctgc atattgatgc tgcccgcgcc ggccgtttgc
       61 gtgtaggtga ccttcagcgt ggtcggcgcg tcggcgcgat acttcacggt gacgacgcgg
      121 ctgtaggtgg tcgtgctcga gaacgtcgtg gtgtacgtcg acgcgccggc catcgtcacg
      181 gtgacgcggc ccgtcgagtt ccatccgccg atgtagtacg tcagcgtgcg ctcgacggtc
      241 gtcgccgcga cggtgaactg aaacgaggcg ttgctgccgg cgaccttgat cgcggtgttg
      301 tcgccgatca agcggggatc gttcgacatc gacttcagtg tgcccgtcgt cgtgacgtcc
      361 gagattgcgg tccctttgtg tgtgtaaccg ggccagcgtg cccagtcgag cgtaccgacc
      421 ttcggcaagt cgacggccgt cgacgaattg acgatcgagc ccgtgagtgt cgcggccact
      481 ccgagcgcag gcgtgaagca agcgagaacc agagcgatca ccgccttcat gcaccttccc
      541 cccgttatgc gttgttgtta ggcggtgcgg acagtaacgg cgaggtgtcg cagcgacgaa
      601 tcgtcggcaa ctggcgacag ctgcgcgccc acaaagccgg ctcggcacaa agcttgcttc
      661 ttcgaggagc actcgcggac aggggagccc acaatgttgc agtcgatcgc agacttgaag
      721 cgctacgccg tctacaacga ccgcggcaac aagctcggtg tcatcgacga cgtcatcctc
      781 gacgccgacg agtggaccgt gtgctacgcc gtcttgcggt acgaggttgc gggcgagccg
      841 accaagctgc ttggcgtggg tctcgacgcg ctaacgctcg acagcgagaa ccagtgtcta
      901 gtcgtcgcgg ccgacgaggc gctgctgagc cgcgcgagcg gcttcgatcg caacgcgccg
      961 ccagcgaagc ccgagccgtt gttcaatagt cccgaccatt agcgctccac cgttgtctcg
     1021 tcctcggtac gccggcctcg ccaccacttg aagcggcgat tgccgcggcg cgacgcctgg
     1081 acgaagaaca gcgcgccgac aaagatcaag acgacgaacc ccaaggcgag gaccgtgtaa
     1141 ctgactgcgt cgccgattcc acccatggca tcacctttgg ttgcgcgaag agaggctgtg
     1201 tgcgatctgc gaccgcgata gatcgccgtc cgcgtggtca ggaggattgg cacggtctat
     1261 cccggtcgct tcgtcgagcg ccgcggtgtc gatgcctacg ctcaaagctc gttggccgct
     1321 gtcgtaaccg agactctcgg gcggtatcgc cagcatcttc gcgccgaccc cgagcactcc
     1381 gccgacggag agcacggcat agacgacgga accgcgctcg gtatcgagca tgaagtcgac
     1441 gatcttgccg agggtctcgc cggccgcgct tctcacgtcg gctgccgaca gcgtattggc
     1501 cgatatcaac cgcacgggtg ctctccacga ccttcgacta cccgtgtgtt gcaagccaca
     1561 taccaaccaa tggcggcgcg gggctgccgg tgaaacggcc gccttcgcgc gcaatgtttg
     1621 gaggtacgtc gcagaaactg cagggtgggc gccgcgaatg gcatgcaact tgctcgagca
     1681 tctcgtctcg aacccgcgga gggcgacgct catgcgcaac gacatcgtgg cgtggttgcc
     1741 gagcatcgcg gccgcgtgcg gcgtcagcgt cctcgccggc tgtggtcatt cggcgagcct
     1801 gacggtggcc gaaggcatgg gcccgcggcc cgagctgccg gcgccggatc gctccttgat
     1861 tcccgtcatc aaaatcgcgc cggctacagg ttggcagaac ggcggcgcac ccgtcgcggc
     1921 agccgggctc agcgtccagc cgttcgcgcg cgatctcgac catccacgct ggctgtacgt
     1981 gctgccaaac ggtgacgtac tcgtcgccga gaccaacgcg ccggaacggc ccgaggatcg
     2041 caaaggcatc aaaggcgcga tcatggcgct cgcgatgaag aaggccg
//
LOCUS       DATLEE010000709         2074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6449240, whole genome shotgun sequence.
ACCESSION   DATLEE010000709 DATLEE010000000
VERSION     DATLEE010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6449240"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VM692_15545"
     CDS             <1..632
                     /locus_tag="VM692_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HUQ53641.1"
                     /translation="VAEDLVAYLDSHYRTLARRESRGIAGHSMGGYGALRIGMKRPDV
                     FSALYALSSCCLNEGTVRVRGEGPSPAESIHSLDEARGNRAAQGTLARAAAWAPNPSN
                     PPLFLDLPTRNGEVVPEVAVKWAANSPVAMLDQYVPNLKKYTAIQLDIGLQDSLLASN
                     EVFVERLNRFHVPHKYLTYEGDHGNRVPQRLEEHVLPFFSQHLSFAQRH"
     gene            635..1843
                     /locus_tag="VM692_15550"
     CDS             635..1843
                     /locus_tag="VM692_15550"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUQ53642.1"
                     /translation="MSRAKLLATLAASLLLHEASAAPRIAPVTEAQRTDAQRALAERF
                     APSGMTNAVATFLQHPALAEAILPYERYISADSKLPPRHRGLLHLRTAWLTRSEYMWA
                     HHAQRALRAGVTRAELARVAAGPTAEGWSAFEAALLRAADELHVDSFVSDATWAALAA
                     EYDTNQMIDLIYGVGEMTMLAGAINTLGVALEGDVAERFPAGIPYTVAAAWKNERLVG
                     KAPRIAPLQPNEWTPVQRARLDPQNTGRAANVFATFARDPPTDALRNAVSGHIRGGST
                     LTPRHRETVIMRIGVLCRSEYEWAAHMRAGKGVGMTDADVERIARGPDAPGDAFEAAL
                     LRATDELYRDDRVSEQTWAVLAAQLDTKQLLDVLIAVGGYRTASMTINSAGVQLDANM
                     ADFRFPAFLR"
BASE COUNT          336 a          735 c          678 g          325 t
ORIGIN      
        1 atgtcgccga ggatctcgtc gcgtacctgg actcgcacta ccgcacgctc gcgcgccgcg
       61 agagccgcgg gatcgccggt cactcgatgg gcggctacgg cgcgctgcgc atcggcatga
      121 agcgccccga cgtattctcg gcgctctacg ctttgtcgtc gtgctgcttg aacgaaggca
      181 cggtccgcgt gcgcggcgaa ggcccctcgc ccgcggaatc gatccactcg ctcgacgaag
      241 cacgcggcaa tcgcgccgcg cagggcacgt tggcgcgcgc cgcagcgtgg gcaccgaacc
      301 cgtcgaatcc gccgctgttc ctcgacctgc cgacgcgcaa cggcgaggtc gtgcccgagg
      361 tcgccgtcaa atgggccgcg aactcgccgg tcgcgatgct cgatcagtac gtgccgaact
      421 tgaagaagta cacggcgatt cagctcgaca tcggcttgca ggacagcttg ctcgccagca
      481 acgaggtctt cgtcgagcgc ttgaaccgct tccacgtgcc acacaagtat ctgacctacg
      541 agggcgacca cggcaatcgc gtcccgcagc gactcgagga gcacgtgctg ccgttcttct
      601 cgcagcactt gtcgttcgcg cagcggcatt gagcgtgtca cgagccaaac tgctggcgac
      661 gctcgcggcg tcgctgttgc tacacgaagc gagcgcggcg ccgcgcattg cgccggtgac
      721 ggaagcgcag cgcaccgacg cgcagcgcgc gcttgcggag cggttcgcgc cttccggcat
      781 gacgaacgcc gtcgcgacct ttcttcagca tcctgcgctc gccgaagcga tcctgccgta
      841 cgagcgctac atctccgccg actcgaagct accgccacgc caccgcggtt tgctgcacct
      901 gcgcacggcg tggctgacgc gctccgaata tatgtgggca caccacgctc agcgcgccct
      961 acgcgccggg gtcacacgcg cggagctcgc gcgagtcgcc gccggcccga cggccgaagg
     1021 ctggagcgcc ttcgaagcag cgctattgcg agcggccgac gagctgcacg tcgattcgtt
     1081 cgtcagcgac gcgacctggg ccgcgctcgc ggccgagtac gacacgaatc aaatgatcga
     1141 tctcatctat ggcgtcggcg agatgacgat gcttgcgggc gcgatcaaca cgctcggcgt
     1201 cgcgctcgaa ggcgacgtcg cggagcgctt ccccgccggc attccgtaca cggtggctgc
     1261 ggcgtggaag aacgagcggc tggtcggcaa agcgccgcgc atcgctcctc tgcagccgaa
     1321 cgaatggacg cccgtgcagc gcgcgcggct cgacccgcaa aacacgggac gcgccgcgaa
     1381 tgtcttcgcg acgttcgcgc gcgatccgcc gaccgacgcc ctgcgcaacg ccgtcagcgg
     1441 ccacattcgc ggcggctcca cattgacgcc gcgccaccgc gagaccgtga tcatgcggat
     1501 cggcgtgctg tgccgctcgg agtatgagtg ggccgcgcac atgcgcgccg gcaaaggtgt
     1561 cggcatgacg gacgccgatg tcgagcgcat cgcgcgcggg cccgacgcgc ccggcgacgc
     1621 cttcgaagcg gccctgctgc gcgcgacgga cgagctctat cgcgacgatc gagtctcgga
     1681 gcaaacgtgg gccgtgctcg ccgcgcaact cgacacgaag cagctgctcg acgtcctgat
     1741 cgccgtcggc ggctatcgca cggcgtcgat gacgatcaac agtgccggcg ttcagctcga
     1801 cgcgaacatg gcggacttcc gcttcccggc cttcctgcgc tgagccgcct tattcccgag
     1861 cgcggcggcg tttgcggctc gccgccgagc cgccgagcag gccgagatcc acgagggcgg
     1921 ccgatgacga cctactgggc gcgttcgtcg tcgaactcga gcagctggtt ctcgaactct
     1981 tcgtaggtcg cgttgtcctc gcagaccagc tcttcgatcg tccaatcggt cttgcgatac
     2041 cagcgcgaga actcccacgg cttcgcgagc gctt
//
LOCUS       DATLEE010000710         3471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7936995, whole genome shotgun sequence.
ACCESSION   DATLEE010000710 DATLEE010000000
VERSION     DATLEE010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7936995"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3471
                     /gene="rpoB"
                     /locus_tag="VM692_15555"
     CDS             <1..>3471
                     /gene="rpoB"
                     /locus_tag="VM692_15555"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HUQ53643.1"
                     /translation="KRIRKDFGKLPGILNVPYLLSIQIDSYQKFLQQEVAGTERTDMG
                     LHAAFRSVFPIVSDSGNAALEYVSYRLGDPVFDVKECQLRGLTYAAPLRVLVRLMIYD
                     KEAPGPKKKVKDIREQEVYLGEMPLMTEHGTFVINGTERVIVSQLHRSPGVFFDHDKG
                     KTHSSGKLLFSARVIPYRGSWLDFEFDPKDSVFVRIDRRRKLPVSIILRALDYTNEQI
                     LDLFFEKNELKLSAEGALLKLVPERLRGETASFDIKIGRKVIVEKDRRITMKHVRELE
                     QAKVKELEVPMEYLEGKILAHDVVDTSSGELLASANDELTKTLVEKLIAAGIKKIETL
                     FVNDLDRGPFISNTLRIDSSTNKLEALVEIYRMMRPGEPPTKEAAENLFQNLFFNTER
                     YDLSAVGRMKFNRRVGRNESEGPGVLSKDDIIAVLKTLIDIRNGNGTVDDIDHLGNRR
                     VRSVGEMAENAFRIGLVRVERAVKERLTLAESEGLMPQEMINAKPVAAAVKEFFGSSQ
                     LSQFMDQNNPLSEVTHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPN
                     IGLINSLSVYARTNEYGFLETPYRRVVDGKVTSEIDYLSAIEEGQYVIAQANANLSPK
                     GEFVDDLVSSRHQNEFALLPAAQIQYMDVSPKQIVSVAASLIPFLEHDDANRALMGSN
                     MQRQAVPTLRTQTPLVGTGMERAVAVDSGVTVVAKRGGIVDSVDASRIVVRVKDEETK
                     AGEAGVDIYNLTKYTRSNQNTCINQRPLVKAGNAIEKGDVLADGPSTSMGELALGQNL
                     LVAFMPWNGYNFEDSILISERIVAEDRYTSIHIEELVVVARDTKLGPEEVTADIPNVG
                     DAALTKLDESGIAFIGAEVGAGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDT
                     SLRVPPGMDGTVIDVRVFTRDGVEKDGRALSIERQELEAVRKDLDDQQRILEDDIYQR
                     IGTALIGKVAAGGPNGLAAGAKITKAYLDELPRDQWFQVRVKADDANAALEQAAERLR
                     SQKALFDKRFDEKKQKITAGDDLAPGVLKMVKVYLAVKRRVQPGDKMAGRHGNKGVIS
                     TIVPVEDMPYAADGTPVDIVLNPLGVPSRMNVGQVLETHLGWAAKGLGLKIGEMIRNG
                     SEVKELRTFI"
BASE COUNT          686 a         1112 c         1140 g          533 t
ORIGIN      
        1 gaagcggatc aggaaggact tcgggaagct gccggggata ctcaacgttc cctacctcct
       61 gtcgatccag atcgattctt atcagaagtt cctgcaacag gaagtcgcgg gcaccgagcg
      121 cacggacatg ggtctgcacg ccgcgtttcg cagcgtgttc ccgatcgtca gcgattcggg
      181 taacgccgcg ctcgagtacg tgagctatcg cctcggcgat ccggtcttcg acgtcaagga
      241 gtgccagctc cgcggcctca cgtatgccgc gccgttgcgc gtgctcgtgc gcctcatgat
      301 ctatgacaag gaagctccgg ggccgaagaa aaaggtcaag gacatccgcg agcaggaggt
      361 ctatctcggc gagatgccgc tgatgaccga gcacggcacg ttcgtcatca acggcaccga
      421 gcgcgtcatc gtctcgcagc tgcacagaag ccccggcgtg ttcttcgatc acgacaaggg
      481 caagacccat tcgtcgggca agctgctgtt ctcggcgcgc gtgattccgt atcgcggctc
      541 gtggctcgat ttcgagttcg atccgaagga ctcggtgttc gtgcgcatcg accggcgccg
      601 caagctgccg gtcagcatca tcctgcgtgc gctcgactac acgaacgagc agatcctcga
      661 tctgttcttc gagaagaacg agctcaagct ctcggccgag ggcgcgctgc tgaagctcgt
      721 gcccgagcga ctgcgcggcg aaacggcgag cttcgacatc aagatcggcc gcaaggtcat
      781 cgtcgagaaa gaccgccgca tcacgatgaa gcacgtccgt gagctcgagc aggccaaggt
      841 caaggagctc gaggtcccga tggagtacct cgagggcaag atccttgcgc acgacgtcgt
      901 cgacacgtcg agcggcgagc tgctcgcaag tgccaacgac gagctgacga aaactctggt
      961 cgagaagctg atcgcggccg gcatcaagaa gatcgagacg ctgttcgtca acgacctcga
     1021 ccgcggcccg ttcatctcga acacgctgcg catcgactcg agcacgaaca agctcgaggc
     1081 gctcgtcgag atctaccgaa tgatgcgccc cggcgagccg ccgacgaagg aggccgccga
     1141 gaacctgttt cagaacctgt tcttcaacac ggagcgctac gacctgtcgg ccgtcggccg
     1201 catgaagttc aatcgccgcg tcggccgcaa cgagtcggaa ggccccggcg tgctcagcaa
     1261 ggacgacatc atcgccgtgc tgaagacgct gatcgacatc cgcaacggca acggcacggt
     1321 cgacgacatc gaccacttgg gcaaccgccg cgtccgcagc gtcggcgaga tggccgagaa
     1381 cgcgttccgc atcggcttgg tgcgcgtcga gcgcgccgtc aaggagcgcc tgactctggc
     1441 cgaatccgaa ggcttgatgc cgcaggagat gatcaacgcg aagcccgtgg ccgcggccgt
     1501 caaggagttc ttcggctcga gccagctgtc gcagttcatg gatcagaaca atccgctgtc
     1561 cgaggtcacg cacaagcggc gcgtctcggc gcttggacca ggcggcctga cgcgcgagcg
     1621 cgccggtttc gaggtccgcg acgtccaccc gacgcactac ggccgcgttt gtccgatcga
     1681 gacgcctgaa ggtccgaaca tcggcctcat caactcgctg tcggtctatg cgcgcacgaa
     1741 cgagtacggc ttcctcgaga cgccgtaccg ccgcgtcgtc gacggcaaag tcacgagcga
     1801 gatcgactac ctgtccgcca tcgaggaagg ccagtacgtc atcgcacagg ccaacgcgaa
     1861 tctgtcgccg aagggcgagt tcgtcgacga cctcgtgtcg tcgcgccatc agaacgagtt
     1921 cgcgctgctg ccggcggccc agatccagta catggacgtc tcgccgaagc agatcgtctc
     1981 ggtcgccgcg tcgctgattc cgttcctcga gcacgacgac gcgaaccgcg cgttgatggg
     2041 ctcgaacatg cagcggcaag cggtcccgac gctgcgcacc cagacgccgc tcgtcggcac
     2101 gggcatggag cgcgccgtgg ccgtcgactc cggcgtcacg gtcgtcgcga agcgcggcgg
     2161 catcgtcgac tccgtcgacg cgtcgcgtat cgtcgtgcgc gtcaaggacg aagagacgaa
     2221 ggccggcgag gccggcgtcg acatctacaa cttgacgaag tacacgcgct cgaaccagaa
     2281 cacctgtatc aatcagcggc cgctcgtcaa agccggcaac gcgatcgaga agggtgacgt
     2341 tctcgccgac ggtccgtcga cgagcatggg cgagctcgcg ctcgggcaaa acctgctcgt
     2401 cgcgttcatg ccgtggaacg gctacaactt cgaggactcg atcctgatct ccgagcgcat
     2461 cgtggccgag gaccgctaca cctcgatcca catcgaggag ctggtggtgg tggcccgcga
     2521 caccaagctc ggccccgagg aagtcacggc cgacattccg aacgtcggcg acgcggcgct
     2581 gacgaagctc gacgagagcg gcatcgcgtt catcggcgcc gaggtcggcg ccggcgacat
     2641 cctcgtcggc aaggtcacgc cgaagggcga gacccagctg acgccggaag agaagctgct
     2701 gcgcgcgatc ttcggcgaga aggcgtcgga cgttaaggac acgagcctgc gagtgccgcc
     2761 cggcatggac ggcaccgtca tcgacgtgcg cgtgttcacg cgtgacggcg tcgagaagga
     2821 cgggcgcgcg ttgtcgatcg agcgccaaga gctcgaggcc gtgcgcaagg acttggacga
     2881 ccagcagcgc atcctcgagg acgacatcta ccagcgcatc ggcacggcgt tgatcggtaa
     2941 ggtcgcggcg ggcggcccga acggcctcgc cgccggcgcc aagatcacga aggcgtatct
     3001 cgacgagctg ccgcgcgatc agtggttcca ggtccgcgtg aaggccgacg acgccaacgc
     3061 ggcgctggag caagcggccg agcggctgcg ctcgcagaag gcgctgttcg acaaacgctt
     3121 cgacgagaag aagcagaaga tcacggcggg cgacgacttg gcgccgggcg tgctgaagat
     3181 ggtcaaggtc tatctcgcgg tgaagcgccg cgtgcagccc ggcgacaaga tggccggccg
     3241 gcacggcaac aagggcgtca tctcgacgat cgtgcccgtg gaggatatgc cgtacgcggc
     3301 cgacggtacg cccgtcgaca tcgtgctgaa ccccctcggc gtgccgtcgc gcatgaacgt
     3361 cggccaggtg ctcgagacgc acttgggctg ggccgcgaaa ggcctggggc tcaagatcgg
     3421 cgagatgatc aggaacggca gcgaggtcaa agagctgcgc acgttcatcg a
//
LOCUS       DATLEE010000711         2493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6450082, whole genome shotgun sequence.
ACCESSION   DATLEE010000711 DATLEE010000000
VERSION     DATLEE010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6450082"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..>2493)
                     /locus_tag="VM692_15560"
     CDS             complement(26..>2493)
                     /locus_tag="VM692_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53644.1"
                     /translation="STVPGDRQRLEDVGFLTCMTLVWLGNYAQTGHFGGPLAYTPFNV
                     VTHLAGRERGGLHFDYRRPKHPYCDKFMLAAGHCAPTCYALWMIMGEALDRKYKATGD
                     RRYYVAPELAMLSVDALGFRRGAGALETLLKDNGLADHPLFAQAKGRGIRALSGHIES
                     TDVTNDVNGGPSGVGLATAAGKAAFWDMLGAGVDSPKIVAFEGEFALCSGHAQELKTI
                     AMAVQAGKRLRIFFSNNNAGIDDALIGGVVPNKFADGYVLRDQWTSYGWNVLDLADGH
                     DYKQILGVLKTMEDWDPADRRPMIVIGKTTKGYWPAKVDKQIVGYQSHPYGFKMNADY
                     IIGLAKTFEARYGVKFEGMSAGPVTDMRERLIQFKTNMDVAMSVLDKNGLGDWLADRL
                     VEIGDRVVDKVKLRVDVTRDPFLDDRLKVANLPVEPQELSVKNAVSGASKTVKCALFR
                     KTGEAAGARRAISEIVKWLNYVTDNRVLTVAADLSESVNLEHGSIWGHYDPETNPLGT
                     RLKAAIQEAGNASTAIGLVSQNASVDPDKFAGVWAFSGTYGAFTPLMYLPARVWSQQN
                     QDSKFKTGVLHILAAHSGPETAADARTHFGIFSPQVWKLFPRGQIINLSFWDYNDVAP
                     GYFAAAEIAARDKKVGIITIEVARPDLPVADRNTFADKDLRAAAKGLYVIRDFAAGPR
                     HGYVVCQGAYATFNTVKNLSKLAEAGVNVKVIAAVSEELFDRQPEAYRRSVLPIGAKQ
                     DMMFVTGGTRRVWPLRNVGAQADDYSLTADWDNQWLTGGLEADVIAEAHLDPNSVFAG
                     VKRFADERGARLKAQRAALDSLE"
BASE COUNT          418 a          814 c          811 g          450 t
ORIGIN      
        1 ggcgaccgtt ggcgctaagg cgccctcact ccaacgagtc gagcgccgcg cgctgcgcct
       61 tcaagcgcgc gccgcgctcg tcggcgaaac gcttgacgcc cgcgaatacc gaattcggat
      121 cgagatgcgc ttcggcgatg acgtcggcct caagaccgcc ggtcaaccat tggttgtccc
      181 agtcagccgt cagcgagtaa tcgtcggctt gcgcgccgac gttacgcagc ggccagacac
      241 ggcgcgtgcc gcccgtaacg aacatcatgt cctgcttcgc accaatcggc agcacggagc
      301 ggcggtaggc ctcgggctga cgatcgaaca gctcttcgct gacagcagca atcactttga
      361 cgttgacgcc ggcctcggcg agcttcgaca gattcttgac ggtgttgaaa gtcgcgtacg
      421 cgccttggca gacgacgtag ccgtggcgcg ggcccgccgc aaagtcgcga atcacgtaga
      481 gacccttcgc cgctgcacgc agatccttgt cggcgaacgt gttgcgatcc gcgaccggca
      541 agtccggccg cgcgacctcg atcgtgatga tgccgacctt cttgtcgcgc gcggcgatct
      601 cggccgccgc gaagtagccg ggcgcgacgt cgttgtagtc ccagaagctc aagttgatga
      661 tctggccgcg cgggaacagc ttccagactt gcggcgagaa aatgccgaag tgggtgcgcg
      721 cgtcggccgc ggtttcggga cccgagtgcg ccgcgaggat gtgcagcacg ccggtcttga
      781 acttgctgtc ctggttctgc tggctccaga cgcgtgccgg cagatacatg agcggcgtga
      841 acgcgccgta cgtgccgctg aacgcccaaa caccggcgaa cttgtcgggg tcgaccgacg
      901 cgttctggct cacgagcccg atcgcggtgg acgcgttgcc ggcctcttgg atcgcggcct
      961 tcaaacgcgt gccgagcggg ttcgtctcgg gatcgtagtg gccccagata ctgccgtgct
     1021 cgaggttgac cgactccgac agatccgcgg ccacggtcag cacgcgattg tccgtgacgt
     1081 agttgagcca cttcacgatc tcggagatcg cgcggcgcgc gccggcggct tcgcccgtct
     1141 tgcggaacag cgcgcacttg acggtcttgc tcgcgcccga cacggcgttc ttgacgctga
     1201 gctcttgcgg ctcgacgggc aagttcgcga ccttgagccg gtcgtcgagg aacggatcac
     1261 gcgtgacgtc gacgcgcagc ttgaccttgt cgacgacgcg gtcgccgatc tcgacgagac
     1321 ggtcggcgag ccaatcgccg aggccgttct tgtcgagcac cgacatcgcc acgtccatat
     1381 tggtcttgaa ctggatcagc cgttcgcgca tgtcggtgac ggggcccgcc gacatgcctt
     1441 cgaacttgac cccgtagcgc gcctcgaacg tcttcgcgag gccgatgatg tagtcggcgt
     1501 tcatcttgaa gccgtacgga tggctctgat aaccgacgat ctgcttgtcg actttcgccg
     1561 gccagtagcc cttcgtcgtc ttgccgatga cgatcatcgg gcgacggtcg gccgggtccc
     1621 agtcctccat cgtcttcagc acgccgagga tctgcttgta gtcatgaccg tcggcgaggt
     1681 cgagcacgtt ccaaccgtac gacgtccatt gatcgcgcag cacgtagccg tcggcgaatt
     1741 tgttcggcac cacgccgccg atcagcgcgt cgtcgatgcc ggcattgttg ttggagaaaa
     1801 agatgcgcag ccgcttgccg gcctgcacgg ccattgcaat cgtcttgagc tcttgagcgt
     1861 gccccgagca cagcgcgaac tcgccctcga aggcgacgat cttcggcgag tcgacgccgg
     1921 ctcctaacat gtcccagaac gcggccttgc cggcggcggt cgcgaggccg acgcccgagg
     1981 gtccgccgtt gacgtcgttc gtgacgtccg tcgattcgat gtgccccgag agcgctcgga
     2041 taccgcgtcc cttggcctga gcgaacaacg ggtgatcggc gaggccgttg tccttcagca
     2101 gcgtctcgag cgcgccggcg ccgcggcgaa agcccagcgc gtcgacggac agcatcgcga
     2161 gctcgggcgc cacgtagtag cggcgatcgc cggtcgcctt gtacttccgg tccagcgctt
     2221 cgcccatgat catccagagc gcgtagcacg tcggagcaca gtggcccgcg gccagcatga
     2281 atttgtcgca ataaggatgc ttggggcggc ggtagtcgaa gtgcagcccg ccgcgctcgc
     2341 ggcccgcgag gtgcgtcacg acgttgaacg gcgtataggc caacggaccg ccgaaatgac
     2401 cggtctgcgc gtagttgccc agccacacga gagtcatgca ggtgagaaaa ccgacgtctt
     2461 ccaggcgttg ccgatcgccc ggcacagtgg aag
//
LOCUS       DATLEE010000712         3143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8818909, whole genome shotgun sequence.
ACCESSION   DATLEE010000712 DATLEE010000000
VERSION     DATLEE010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8818909"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VM692_15565"
     CDS             complement(<1..212)
                     /locus_tag="VM692_15565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ53645.1"
                     /translation="MIRACTAADAAAICSIYNPYVLETVITFEEAPVATGEMTQRIAD
                     VTALYPWLVAEANGAVVGYAYATPWKT"
     gene            complement(209..592)
                     /locus_tag="VM692_15570"
     CDS             complement(209..592)
                     /locus_tag="VM692_15570"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HUQ53646.1"
                     /translation="MSIDELLAGYPEPVRETAAAARELLQTALPNIKESVDESAKLIG
                     FSYGPGYKGLLCTLIMSKTGVKLGLFRGAELPDPKKLMAGEGKVHRHVQLRSPADVDK
                     PGLKGLLKAALNAWQARTAEAQAKR"
     gene            complement(592..942)
                     /locus_tag="VM692_15575"
     CDS             complement(592..942)
                     /locus_tag="VM692_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019508466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ53647.1"
                     /translation="MTRSEIAREERPARGIRFSIRNERGEVARAYLYVMTNDLHAEPF
                     GLLEDVFVAESERGSGLGTALVNEVIAAAREAGCYKLVATSRVSRPKVHELYERLGFA
                     NYGLEFRLNLAGAD"
     gene            complement(929..1627)
                     /locus_tag="VM692_15580"
     CDS             complement(929..1627)
                     /locus_tag="VM692_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53648.1"
                     /translation="MTAPAIGGAAILLAVALVWYLRPQPEISVETADAPQPRAQVTGS
                     PAPRTAEPSSAESARATPTESVKSPVSERAARSPLPGETPAAPMAELFANRQQNVVVQ
                     RGDANSGVPPQVAEGEREFAAEPVDATWASGAEAALLAKFAQMPGLELIDLQVECRST
                     MCRLQVTQPRAVTEVGASPFNLLYNDSGMKPRWMMVVPDGPLGGPGPMRSIAYLWRDG
                     FAPEREQGAAHDAK"
     gene            complement(1653..2015)
                     /locus_tag="VM692_15585"
     CDS             complement(1653..2015)
                     /locus_tag="VM692_15585"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HUQ53649.1"
                     /translation="MAERFGIETSSTMKLAEVRRMALALPEATEEPHFDLTSFRVKGK
                     IFATVAPDGSYMNVFVDDAQREIMTAVDSKAYESLLWGKTSYLHVHLAAAKANDVKTL
                     LRSAWERKAPKKLLAATG"
     gene            complement(2056..2403)
                     /locus_tag="VM692_15590"
     CDS             complement(2056..2403)
                     /locus_tag="VM692_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ53650.1"
                     /translation="MSTHHTIDYIEFTVRDLGVAKRFYAAAFGWQFNDYGPEYAGIKG
                     GTGEVGGLHQTSDARTTGPLVILYSKDLDRTVAAVKSAGGKILREPYAFPGGRRFHFT
                     DPSGNELGVWSEK"
     gene            complement(2489..2707)
                     /locus_tag="VM692_15595"
     CDS             complement(2489..2707)
                     /locus_tag="VM692_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53651.1"
                     /translation="MGQHRFNVFGQLVVIKGAPGNWSAFLLGPDGKRRAADFVVPSSL
                     AEDELCRYLSDLFHEHASSTQHSVTRIG"
     gene            complement(2786..>3143)
                     /locus_tag="VM692_15600"
     CDS             complement(2786..>3143)
                     /locus_tag="VM692_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53652.1"
                     /translation="IASFVHFAHNAEYLAEYPNLPASLTRVQVYAVWVGTAALGVLGY
                     GLLLKRRQLLGLGVLAVYAAIGFDGLLHYTRAPLAAHTGTMNLTIWSEVVAAALALAA
                     VLALWIEEGRGRAPAR"
BASE COUNT          511 a         1078 c         1003 g          551 t
ORIGIN      
        1 gtcttccacg gcgtcgcata ggcgtagccg acgaccgcgc cgttcgcctc ggcgacgagc
       61 cacgggtaga gcgccgtgac atcggcgatg cgttgcgtca tctcgcccgt cgccaccggc
      121 gcttcctcga acgtgatgac cgtctcgagc acgtacgggt tgtagatcga gcagatcgcc
      181 gcggcgtcgg cggccgtgca cgcgcgaatc atcgcttcgc ttgcgcctcc gcggtgcgcg
      241 cctgccacgc gttcagcgcg gccttcaaca accccttcaa gcccggcttg tcgacgtcgg
      301 cggggctgcg caattgcacg tggcgatgga ctttgccctc gccggccatg agcttcttcg
      361 ggtccggcag ctccgcaccg cgaaagaggc cgagcttgac gccggtcttg ctcatgatca
      421 gcgtgcagag cagccctttg tagccggggc cgtagctgaa gccgatcagc ttcgcggact
      481 cgtcgaccga ctcctttatg ttcggcagtg cggtctgcaa cagctcgcgc gccgcggccg
      541 ccgtctcgcg aaccggctcc ggataaccgg ccagcagctc gtcgatcgac atcaatcggc
      601 tcctgcaaga ttcaagcgaa actcgagccc gtaattggcg aagcccaagc gctcgtaaag
      661 ctcgtgcacc ttcggccgcg acacgcggct cgtcgcgacg agcttgtaac aacccgcctc
      721 gcgcgccgcc gcgatgactt cgttgacgag cgccgtgcca agcccgctac cgcgctccga
      781 ctcggcgacg aacacgtcct cgagcaagcc gaagggctcg gcatgcaagt cgttcgtcat
      841 cacgtacagg tacgcgcgcg cgacttcgcc gcgctcgttg cgaatcgaga aacgaatgcc
      901 gcgcgccggc cgctcttcgc gtgcgatctc acttcgcgtc atgcgccgcg ccttgttccc
      961 gctccggggc gaaaccatcg cgccacaagt acgcgatcga tctcatcggg ccggggccgc
     1021 cgaggggccc gtcgggaacg accatcatcc agcgcggctt catgccgcta tcgttgtaca
     1081 agagattgaa gggcgacgcc cctacctccg ttacggcacg cggctgagtc acttgcaatc
     1141 ggcacatcgt ggaccggcac tcgacctgca ggtcgatcag ctcgaggccc ggcatctgcg
     1201 cgaatttggc caacagagcg gcttcggcgc cgcttgccca cgtcgcgtcg accggctcgg
     1261 ccgcgaactc gcgctcgcct tctgcgacct gcggcggaac gccgctgttc gcgtcgccgc
     1321 gttgcacgac cacgttctgc tgtcgattcg caaacagctc ggccatcggc gcggccggcg
     1381 tctcgccggg cagcggcgat cgggccgcgc gctccgacac ggggcttttg accgactcgg
     1441 tcggcgttgc ccgcgcgctc tcggctgagc ttggctccgc agtgcgtgga gcagggctgc
     1501 cagttacttg cgcgcgcggc tgcggcgcgt cggccgtctc gacgctgatt tccggttgcg
     1561 gccgcagata ccaaacgagc gcgaccgcga gcaggatcgc tgcaccgccg atcgccggag
     1621 ccgtcatgcg cacggaggta tgtccacaca catcagccgg tggccgcgag caacttcttc
     1681 ggcgctttgc gctcccacgc cgagcgcagc agcgtcttca cgtcgttggc tttcgccgcc
     1741 gcgaggtgca cgtgcaggta agacgtcttg ccccacagca gcgactcgta cgctttggag
     1801 tcgaccgcgg tcatgatctc gcgctgcgcg tcgtccacga acacgttcat gtagctgccg
     1861 tcgggcgcga cggttgcgaa aatcttgccc tttacgcgga acgaggtgag gtcgaagtgc
     1921 ggctcctcgg tcgcttccgg gagcgccaac gccatacgac gcacttccgc gagtttcatc
     1981 gtgctgctcg tctcgatgcc gaaacgctcg gccacagtac gctcaacttc cgcgcgaggt
     2041 aaagcaccgc gcgctctact tctcggacca gacgccgagc tcgttgccgc ttggatccgt
     2101 gaagtgaaaa cgccgcccgc cgggaaaggc gtacggctcg cgcaggatct tgccgcccgc
     2161 cgatttgacg gccgcaacgg tcctgtcgag gtccttcgag tacaggatca cgagcggccc
     2221 ggtcgttcga gcgtcgctcg tctggtgcag gccgccgacc tcgcccgttc cgcctttgat
     2281 gcccgcgtac tcgggcccgt agtcgttgaa ctgccagccg aaagccgcgg cgtaaaagcg
     2341 cttggctacg cccaagtcgc gcacggtgaa ttcgatgtag tcgatcgtgt gatgggtgct
     2401 cacggctcgc tcctttgcgg cggatacggc cagagtacct tcaactgttg accgctcgtg
     2461 tcatgttcga cacgcgaagg cgggcgaatc aaccgatccg agtgacggaa tgctgcgtcg
     2521 agctcgcgtg ctcgtgaaag aggtcggata ggtagcggca gagctcgtcc tcggccaagg
     2581 agcttgggac cacgaaatcg gcggcgcggc gtttgccgtc gggccccagc aggaacgccg
     2641 accagttccc cggcgcgcct tttatgacca cgagctggcc gaagacgttg aagcggtgct
     2701 gccccatctt ggatatctgc gaaatgaaca cgagatggcc ccagcccctt tgatgcggct
     2761 tggggcagat tggttacacc ttcgactacc tcgccggtgc ccggccgcgt ccttcctcga
     2821 tccagagcgc gaggaccgcg gccagcgcca gcgcggcggc cacgacttcc gaccaaatcg
     2881 tcaggttcat ggttcccgta tgcgcggcga gcggggcgcg cgtgtagtgc agcaggccgt
     2941 cgaagccgat cgcggcgtag acggccagga cgccgagacc gaggagctgc cgccgcttca
     3001 gcaaaaggcc gtagccgagt accccgagcg ccgcggtccc gacccagacg gcgtagacct
     3061 gcactcgcgt caacgaggcc ggcagattcg gatattccgc caggtactcg gcgttgtgcg
     3121 cgaagtgcac gaagctcgcg atc
//
LOCUS       DATLEE010000713         2243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7942582, whole genome shotgun sequence.
ACCESSION   DATLEE010000713 DATLEE010000000
VERSION     DATLEE010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7942582"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VM692_15605"
     CDS             complement(<1..828)
                     /locus_tag="VM692_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUQ53653.1"
                     /translation="MAVLAALRATAWPFNDGETPTLAPLLQRVTPGVVNISVSGSAAE
                     ARNPLLEDPFFRRFFEGPDAPLAPPSVPRQSIGSGVIIDADEGLVVTNHHVVQGADSI
                     VVTLNDRREYNATLVGSDAGTDIALLRVVADAKLTEIPIGNSSQLAVGDYVVAIGNPF
                     GLGQTATAGIVSALGRSGVNIESFEDFIQTDASINPGNSGGALVDINGQLLGVNTAIL
                     SGTGGNIGIGFAIPSNMVREVVAQLLEHGNVERGRIGIAIQDVTPGLAQALELEVDSG
                     "
     gene            complement(870..1598)
                     /locus_tag="VM692_15610"
     CDS             complement(870..1598)
                     /locus_tag="VM692_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53654.1"
                     /translation="MKLLITGACVLLTACAASRTTIQSQWADDAYSGPPLDRLAVVAL
                     FDTRAESLSFEQSAADYLAAQGIETVPGHALLSPEDGRTLDEAEVRDRVAATDVDGLL
                     IFRLVAVDERREYQPATPYLNVPPDVATGDLSSWYYTPSPTSSFYWYWRSSADVTAAP
                     GYWIEQTFLVAETALFDNRNDRLLWTAKSATMDDARFQRTSESIVRAVARELFDADLI
                     APMAAAAARGAERQPPELTREGRT"
     gene            1782..>2243
                     /locus_tag="VM692_15615"
     CDS             1782..>2243
                     /locus_tag="VM692_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HUQ53655.1"
                     /translation="MNGKIFVHCAAVLSAAALVVGCAPDSPEGGGANRDRPGNGALSR
                     NDQAPNAADRDNGASIRPPTTTTSPGAPGAAPPAAGAAAPPAATPRLMARADVRPLGD
                     NTARGIVEFQTAAGGSGPMTIHVMLMGLDAGPHGMHVHMGTDCAMPGEHLNT"
BASE COUNT          366 a          737 c          774 g          366 t
ORIGIN      
        1 gccgctgtcg acctcgagct cgagcgcttg cgcgagtccc ggtgtcacgt cctggattgc
       61 gatgccgatg cgaccgcgtt ccacgttccc gtgctcgagc agttgcgcaa cgacctcgcg
      121 caccatgttg ctcgggatcg cgaagccgat gccgatgttg ccgcccgtgc cggacagaat
      181 cgccgtgttc acgccgagca gctgaccatt gatgtcgacg agggcgccgc ccgagttgcc
      241 cgggttgatc gaagcgtcgg tctgaatgaa atcctcgaaa ctttcgatgt tgacgccgct
      301 gcggccgagc gcgctgacga tgccggctgt cgcggtctgg ccgaggccga atgggttgcc
      361 gatggcgacg acgtagtcgc cgacggcgag ctggctcgag ttgccgatcg gaatctcggt
      421 gagcttcgcg tccgcgacga cgcgcagcaa ggcaatgtcg gtgccggcgt cgctgccgac
      481 gagcgtcgcg ttgtactcgc gccgatcgtt cagcgtgacg acgatgctgt ccgcgccttg
      541 tacgacatga tggttcgtga cgacgagccc ttcgtcggcg tcgatgatca cgccggagcc
      601 gatgctctgg cgcgggaccg acggtggcgc aagcggcgcg tcggggccct cgaagaagcg
      661 ccggaagaac gggtcctcga gcagcggatt gcgcgcttcg gcagcggagc cggagaccga
      721 aatgttcacg acgcccgggg tgacgcgctg caatagcggc gcgagggtcg gtgtctcgcc
      781 gtcgttgaat ggccatgccg tggcgcgcag cgccgcgagc accgcgatcg cgataatcgt
      841 cgccgtcgcg atcaacaata ggttccgttt cacgttctgc cctccctggt gagctccggc
      901 ggctgccgct ctgcaccccg agcggcagcg gcggccatag gtgcgatgag atccgcgtcg
      961 aacagctcgc gtgcgacggc tcggacgatc gattcggatg tacgctgaaa gcgcgcgtcg
     1021 tccatcgttg ccgacttcgc ggtccacagc agtcggtcgt tgcggttgtc gaacaacgcg
     1081 gtttcggcga cgaggaaggt ctgctcgatc caatagcccg gcgcggccgt gacatcggcc
     1141 gacgagcgcc aataccagta gaagctcgag gtcgggctcg gcgtgtagta ccaggacgag
     1201 aggtcgcccg tggcgacgtc cggcggaacg ttcaagtacg gcgtcgccgg ctgatactcg
     1261 cgccgttcgt cgacggcgac caaccgaaag atcagcaggc cgtcgacgtc ggttgctgcg
     1321 acgcgatccc ggacctcggc ctcgtcgagg gtgcgcccgt cctccggcga caacagcgcg
     1381 tgccccggaa ccgtctcgat gccttgcgcc gcgagatagt cggcggcgct ttgctcgaag
     1441 ctcaggcttt ccgcacgcgt gtcgaatagc gcgacaacag cgagccggtc gagcggcggg
     1501 cccgagtaag cgtcgtcggc ccattgcgac tggatcgtcg tgcgactcgc ggcgcaggcc
     1561 gtgagcagca cgcacgctcc ggttatcagt aatttcattg cggctccttc gggtggtcga
     1621 gcaagttcta tgccacgcag cgcgcaggcc gaatccgcat ttgccccggg cgctaacggt
     1681 cgcggctcgc gccacaatcg ccgacgcgcg agcacgcgcc gccgctctgg caccaatctt
     1741 gctggtgtgc ggcacatcca gaagataagt ggagacggca aatgaacggc aagatcttcg
     1801 tgcattgcgc cgctgtgctc agcgcggctg cgctggtcgt cggttgcgca ccggacagtc
     1861 cggagggcgg cggtgcgaac cgcgaccgcc ccggcaacgg tgcgttgtcg cgtaacgacc
     1921 aagctccgaa cgcggcagat cgcgataacg gcgcctcgat tcggccaccg actacgacca
     1981 cgagcccggg cgctcccggc gcggcgccac cggcggccgg cgcagcggca ccaccggcgg
     2041 ctacaccgcg actcatggcg cgcgccgacg tgcggccgct cggcgataac accgcacgcg
     2101 gcatcgtcga gtttcagacg gcggcgggcg gcagcggccc gatgacgatc cacgtaatgc
     2161 tcatggggct cgatgcgggc ccgcacggca tgcacgttca catgggcacg gactgcgcca
     2221 tgcccggcga gcacttgaat acg
//
LOCUS       DATLEE010000714         2786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3624564, whole genome shotgun sequence.
ACCESSION   DATLEE010000714 DATLEE010000000
VERSION     DATLEE010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3624564"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(676..1038)
                     /locus_tag="VM692_15620"
     CDS             complement(676..1038)
                     /locus_tag="VM692_15620"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53656.1"
                     /translation="MTISVGSGALLVAIASFSTAAWAHHSAAQFDFAQTVRVEGVVKE
                     MRVANPHIALFLEVTDAKGKRVIEYEGHSRNNVYRRGWRPEMVHEGDAISIDIAPMRT
                     GEDGGYVKTFITKDGTEF"
     gene            1424..1777
                     /locus_tag="VM692_15625"
     CDS             1424..1777
                     /locus_tag="VM692_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53657.1"
                     /translation="MAKTTKIDEVGFAGRSGQRYEFRVYVWETKFKAVPGVYVVASRT
                     LEPGKKAHYSPLFAGEAQDLSSALKKHPRDECFQMYYGNVIGVLKEDDDRRRASILAD
                     LVDGLAPPCNAADAP"
     gene            1778..2053
                     /locus_tag="VM692_15630"
     CDS             1778..2053
                     /locus_tag="VM692_15630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ53658.1"
                     /translation="MLAIRHDSTAQRFTADIDGATAYISYRATPGRVLDLHHTYVPRA
                     ARGGGIASQLTAHVLMFARDEGYKVVPSCPFVAAYIDRHAEYRDLLA"
     gene            2233..>2786
                     /locus_tag="VM692_15635"
     CDS             2233..>2786
                     /locus_tag="VM692_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015828310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HUQ53659.1"
                     /translation="MSARSSVTRPASLRQSAPLRQPLSLAIAAVLASSFAATAQAQPD
                     QPETILVTATRRELSVQDIPLNIAAFDDSLLEAREISDLAELGRNVPGLYVIDQGKRS
                     ANSIVVRGLNLNPFASAEFLGNSGGDTVATYVGEVPLYVDLSLNDIERVEVLLGPQGT
                     LYGAGTLGGAIRYIPRRPQFDDTTV"
BASE COUNT          513 a          937 c          831 g          505 t
ORIGIN      
        1 cggccttgcc ccctcgccgc tcatatgggc ttcggtcagc gccctgacgc agtcctggtg
       61 agacagacac ccggcggcgc ccacccaaag cctcgcgtgg acactcgcga accgcacgcc
      121 agccttgaga gttcttgact acctctaaca gtctcaccct ctatacttac ttccgctttg
      181 agaatttcct ccccgattgg agatcttgaa gccccctgta gagctcgacc cccaagtcgg
      241 gctctctttt tttttgcgct acactcgcgc cagcgcacca gcgtcgagac gttcgatgcc
      301 ttccggctcc gacagtggat ccgctccgtc aaagacaacc cggccggtag ggtcgttgcc
      361 gaccccgaag gcaatcgctg ggagtggcag tcggaagacg agacggcgca tctcctgaaa
      421 aagctgagca atgacgagtt gtcgatcgaa aagacggaca ttcgtccgtc actgaaagat
      481 cccgcccgta atgcggcagc cgcgcgatcg cctagggcgg cgggcaaacc gctgcgaccg
      541 agcgttcccg acaagggcgg cggcttcaat ccctatgaca actccggcaa accgaaacgc
      601 cgttgacgcc aaaatgacga accgcgcgcg gagccagagc tcggcgcgcc gttgctgtcc
      661 gctcgctaac ggggattaga actcggtgcc gtccttcgtg atgaaggtct tcacgtagcc
      721 gccatcctca ccggtgcgca tcggcgcgat gtcgatgctg atggcgtcgc cctcgtgaac
      781 catctcgggt cgccagccgc ggcggtagac gttgttgcgg ctgtgacctt cgtactcgat
      841 gactcgcttg cctttggcgt ccgtgacctc gaggaacaac gcgatgtgcg ggttcgccac
      901 gcgcatctcc ttgaccacgc cttcgacgcg caccgtctga gcgaagtcga attgggcggc
      961 agagtggtga gcccacgccg ccgtcgagaa cgaggcgatc gcaaccagca acgctcccga
     1021 gccaacgctg atcgtcatcc gagcactccg tgacacccag agtcgcaaag tataggcgct
     1081 ctcgtgcgca cctcatctaa agaatgccgg cgttggccgc taaaccaacg ccggcatcca
     1141 ccccccgcaa atcggttgtc gccgccggca cagtcgcgcg cggagctcga cgcggcgcga
     1201 ccgcaatcgg cggtggctcg gacaacgcgc tcgtcgagtc gattaactgc ggctgttgct
     1261 cgctgcaact atcagtacgc tggtggcgag cctaccactt gaccgcgcgc agcagcggac
     1321 cggatggttc ggctcagtgt ggcgaggtcg cgacgcaggc tggtatacag gagccaatgc
     1381 gaacgcttct ataattcgca ccgttgccga accggcgagg accatggcca agactacaaa
     1441 gatcgacgag gtggggttcg caggccgctc gggacagcgc tacgaatttc gcgtctacgt
     1501 gtgggaaacg aaattcaaag cagtcccggg cgtctacgtc gtggcgtcgc gcaccctcga
     1561 gcccggcaag aaagcgcact actccccgct gttcgccggc gaggcgcagg atctttcgtc
     1621 ggccttgaag aagcatcctc gagacgaatg ctttcagatg tactacggca acgtcatcgg
     1681 cgtgctcaag gaagacgacg accgaaggcg cgcgtcgatt cttgctgacc tcgtcgacgg
     1741 gctagcacca ccttgcaacg ccgcggacgc tccgtgaatg ctcgccatac gccatgattc
     1801 gaccgcgcag cggttcacgg ccgacattga cggcgcgacg gcgtacatca gttaccgtgc
     1861 aacgccgggg cgtgtgctcg atctgcacca tacctacgtg ccgcgtgccg cacgcggtgg
     1921 cggtattgcg tcacagttga cggctcacgt cctcatgttc gcgcgcgatg aaggctacaa
     1981 agtcgtgccg agttgcccgt tcgtcgccgc ctacatcgac cggcacgctg agtaccgcga
     2041 tctgctggcc tgaggtaccg tcagcgccga cacctgcccg cgctgcatga gttgttgtga
     2101 caacgcatcg ccggacgcct tggtttgaga cggctgtcgc gtcggtgccg cattgggctc
     2161 ggctacagtg accgcctggt tttgggtgcg tcgctaagcg cccgacctca tgtcgattca
     2221 ggaggactgc ccatgagcgc ccgctcgtcc gtcacgcgcc cagcttcgct gcgccaaagc
     2281 gccccgctac gccaacctct ctcgctcgcg atcgctgccg tgttggcctc gagcttcgca
     2341 gcgaccgcgc aagctcagcc cgaccagccc gagacgattc tcgtgacggc gacgcgccgc
     2401 gagctcagcg tgcaggatat tccgctgaac atcgccgcgt tcgacgactc gttgctcgaa
     2461 gcgcgcgaga tcagcgacct cgcggagctc ggccgcaacg ttcccggcct ctacgtcatc
     2521 gatcaaggca aacgctctgc caacagcatc gtcgttcgcg gcttgaatct gaacccgttc
     2581 gcgtcggccg agttcctcgg caactccggc ggtgacaccg tcgcgactta cgtcggcgaa
     2641 gtgcccttgt acgtcgacct tagtctcaac gacatcgagc gagtcgaagt actgctcggc
     2701 ccgcagggca cgctctacgg cgccggcacg ctcgggggcg cgatccgcta catcccgcgc
     2761 aggccgcagt ttgacgacac gacggt
//
LOCUS       DATLEE010000715         7776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7336456, whole genome shotgun sequence.
ACCESSION   DATLEE010000715 DATLEE010000000
VERSION     DATLEE010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7336456"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VM692_15640"
     CDS             <1..348
                     /locus_tag="VM692_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HUQ53660.1"
                     /translation="AMQRAVLRLKLVPELVRVDGRQIPWFAGHARLYRAEPIIDGDNL
                     VPEISAASILAKVCRDRLMRRLHRRYPQYGFDQNKGYATAEHLRALEQFGPCAIHRAT
                     FAPVRESGAEHAG"
     gene            345..3806
                     /gene="dnaE"
                     /locus_tag="VM692_15645"
     CDS             345..3806
                     /gene="dnaE"
                     /locus_tag="VM692_15645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HUQ53661.1"
                     /translation="MNSFVHLRVHTEYSLVDSLLRVDALVDAVAEQRMPACAITDQGN
                     VSALVKFYKPALSRGVKPIVGADVWVADSAEDREPARLTLLCQNRAGFKRLSSLLTKS
                     AVQGPISGRNVLLKSWLEPAALDGLIGLSGGQHGELGRALAAGRGTRGSEVLAYWQQL
                     LPGRYYVELQRLGRAGEREYLARAVEAAAAHAVPVVATNDVCFLERGDYEAHETRVCI
                     AQGVTLDDPSRARTYSEQQYLRTPAEMAALFADLPEAVANTVEIAKRCSLEVDVGRVF
                     LPDFVAEDRTPPRQLLERRSASGLEQRIAELKLPAEVLPRYRERLTREIDVICKMGFE
                     GYFLIVADFIAWARTNNIPVGPGRGSGVGSLVAYALGITNLDPLAHDLLFERFLNPER
                     VSLPDFDIDFCIDGRDRVIDYVGRRYGRERVAQIATYGTMAARAVVRDVGRALGMPYG
                     YVDRIAKLIPFEIGITLDKAIADDEELRKLYENDEEIRGLIDLAKRLEGLARNVGTHA
                     GGVVIAPQPITEYMPLYADADGALLTQLDKDDLEAIGLIKFDFLGLKTLTIIDKAVAT
                     INRARASRSEPPLNVDAIPVDDAKTYELLRQCRTTAVFQLESLGMRDLIKRLQPDRFD
                     DLTAIVALFRPGPMQMADEFIERKHGTGGARDFLHPRLETTLKPTYGVILYQEQVMQI
                     AQILAGYSLGGADLLRRAMGKKKPEEMAQQRAVFLKGATERGVEESRANHIFDQMETF
                     AGYGFNKSHAAAYALIAYQTAWLKTHYPEDYMAAVLTADSDNTDRLVVLKDELKRMGI
                     ALEPPNVNASVLPFTVAGPKRISYGLGALKGVGPSAVEAIVAERAHGPYKSLVDLCRR
                     VDLQKINRRVLEALAKSGGLDSLGANRATLMNGISNALQAAERSVHAQAAGQTALFGG
                     DERDDTLEHVLTPVREWSKRERLEGERESLGLYLTGHPFDDFAEHCKHFTNGAIAKVL
                     QGLPSSALPYHVRKEAILAGVVVDVRRRGNRVSIILDDDTERIEVTMFDEVFAQSKHV
                     IAKHAVLIAEGQLRYDDFINGWRLTAKRVRSADEAIEEYARRLTIRWPSDGVGTELVR
                     ELQRILKPFTRGRCEVSIEYRRNGAEATLTLGEAWSVRCTRELRDQLTRLLGDDRLLV
                     HYPKHFV"
     gene            4394..5353
                     /locus_tag="VM692_15650"
     CDS             4394..5353
                     /locus_tag="VM692_15650"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HUQ53662.1"
                     /translation="MDLKFLEFEQPIAELEARIDALKFVGEDSELNISEEISRLRNKS
                     ESLTRSIFSNLTAWQIAQLARHPQRPYTLDYVDKCFTDFQELHGDRMYADDHSLVGGL
                     ARLDNEPVVVIGHQKGRDTKERVWRNYGMARPEGYRKGLRLMHLAEKFGLPLITLIDT
                     PGAYPGIGAEERGQSEAIARNLFVMSSLRVPIVSVVIGEGGSGGALAIGVADRVLMLQ
                     YSIYSVISPEGCASILWKSADKAELAAEAMGITAERLHRLKLVDEVIAEPLGGAHRDP
                     QVTADALKHALAQTLATLQAIPLDSLLVQRRKRLASYGVFKES"
     gene            5386..6762
                     /gene="tilS"
                     /locus_tag="VM692_15655"
     CDS             5386..6762
                     /gene="tilS"
                     /locus_tag="VM692_15655"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HUQ53663.1"
                     /translation="MTTPPPPQADDVTAVVRATLERWASRELPPRITVAFSGGLDSTV
                     LLGALCRLALPVRIRAAHIDHGLQPQSAEWSEHCAATAAALGAQFVTARVAVDQTAGL
                     GLEAAARDVRYRTLGELMLAGEWLLTAHHADDQLETLLLRFMRGTGVRGLRGIIPFGP
                     FGVGALGRPLLSFTRAQLHARALEWNLRWLEDPSNREPRQDRNYLRLHVVPALLARWP
                     DAAQHAERLAEQAGEAERLLDAVAAEDARPLVAPWHVPRTVLAALEPARQRNLLRHVL
                     RAVGLGTPSARKLEELRAALLEAHADSHTQVSWPGAEGRVFRDALYLGVPPAAHSPAD
                     HAARIDSAAAWSGPEGRVELLPVQTSPGVPESWLAEGLTLRFRAGGERFRPRGRQHHH
                     SLKDLFQEHGVVPWMRDRVPLLYRGAALVAIGDLWLSADVDAAPATEPRYSVRWTEHP
                     PVRAPEKS"
     gene            6904..>7776
                     /locus_tag="VM692_15660"
     CDS             6904..>7776
                     /locus_tag="VM692_15660"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HUQ53664.1"
                     /translation="MTRFIFITGGVVSSLGKGITSACLGAILEARGLKVRLMKLDPYI
                     NVDPGTMSPFQHGEVYVTDDGTETDLDLGHYERFVHTRTNRMSNFTTGKIYSNVIAKE
                     RRGEYLGATVQVIPHITDEIKRNVIAGAGDADVCMVEIGGTVGDIESLPFLEAIRQMG
                     VELGRDRALYIHLTLVPFMAASSEIKTKPTQHSVKELRGIGIQPDVLVCRSEHALPRE
                     QRAKIALFTNVEERAVISAVDVDDIYKIPMLLAEQGLDSYVVDKLRLDARPTDLSEWQ
                     AVVDAKAHPEAEVTV"
BASE COUNT         1275 a         2603 c         2651 g         1247 t
ORIGIN      
        1 gccatgcagc gcgccgtgct gaggctcaag ctcgtgcccg agctcgtgcg cgtcgatggc
       61 cggcagatcc cgtggttcgc cgggcacgcg cgcctgtatc gcgccgagcc gatcatcgac
      121 ggcgacaatc tggtgccgga gatcagcgcc gcgtcgattc ttgcgaaggt gtgccgcgac
      181 cggctcatgc gccgcctgca tcgccgttat ccgcaatacg gtttcgatca aaacaagggt
      241 tatgccaccg cggagcactt gcgcgcgctc gagcagttcg ggccgtgcgc gattcaccgt
      301 gcgacgttcg cgcccgtgcg cgagagtggc gcagagcatg cgggatgaac agcttcgttc
      361 atttgcgcgt ccatacggag tactcgctcg tcgatagcct gttacgcgtc gatgcgctcg
      421 tcgacgccgt agccgagcag cgcatgccgg cttgcgcaat cacggatcaa ggcaacgtct
      481 cggcgctcgt caagttctac aagcccgcac tgtcgcgcgg cgtcaagccg atcgtcggcg
      541 ccgacgtgtg ggtcgcggat tctgccgagg atcgcgagcc ggcgcggctg acgctgctgt
      601 gtcagaaccg tgcgggcttc aaacgattga gctcgttgct gacgaagagc gccgtccaag
      661 ggccgatcag cggccgcaac gtcctgctca agagttggct cgagccggcc gcgctcgatg
      721 ggctgatcgg cctgtcgggc ggccagcacg gcgagctcgg cagggcgctt gccgcgggcc
      781 gcggcacgcg cggctccgag gtgctcgctt actggcaaca gcttttgccg ggccgctact
      841 acgtcgagct gcagcggctc ggccgcgccg gcgagcgcga atatctggcg cgcgccgtcg
      901 aggctgccgc ggcgcacgcc gtgcccgtcg tcgcgacgaa cgacgtctgc ttcctcgagc
      961 gcggcgacta cgaggcgcac gagacacgcg tctgcatcgc acaaggcgtc acgctcgacg
     1021 atccgagccg ggcgcggacc tacagcgagc aacagtacct gcgcacgccg gcggagatgg
     1081 cggcgttgtt cgccgacctt ccggaggccg tggcgaatac cgtcgagatc gcgaagcgct
     1141 gctcgctcga ggtcgacgtc ggccgcgttt ttttgcccga cttcgtcgcc gaggatcgca
     1201 cgccgccgcg ccagctgctc gagcggcgct ccgcgagcgg cctcgagcag cgcatcgcgg
     1261 agctcaagct gccggcggaa gtgctgccgc gctatcgcga gcgtttgacg cgcgagatcg
     1321 acgtcatctg caagatgggc ttcgagggct acttcctgat cgtcgccgac ttcatcgcct
     1381 gggcgcgcac caacaatatt cccgtcgggc cgggccgcgg ctcgggcgtc ggctcgctcg
     1441 tcgcctacgc tctcggcatc acgaacttgg atccgctcgc gcacgatctc ctgttcgagc
     1501 gattcttgaa tcccgagcgc gtttcgctgc cggacttcga catcgacttt tgcatcgacg
     1561 gccgcgatcg cgtcatcgac tacgtcggcc gccgctacgg ccgcgagcga gtcgcgcaaa
     1621 tcgcgacgta cggcacgatg gcggcgcgcg cggtggttcg cgacgtgggc cgcgcgctcg
     1681 gcatgccgta cggctacgtc gaccgcatcg cgaagctgat tccgttcgag atcggcatca
     1741 cgctcgacaa agcgatcgcc gacgacgagg agctacgcaa gctttacgag aacgacgagg
     1801 agatccgggg cctcatcgat cttgctaaac gcctcgaggg actggcgcgc aacgtcggca
     1861 cgcacgcagg cggcgtcgtg atcgcgccgc agccgatcac cgagtacatg ccgctctacg
     1921 ccgacgcgga cggcgcgctg ctgacgcagc tcgacaagga cgatctcgag gcgatcgggc
     1981 tcatcaagtt cgacttcctc ggcttgaaga cgttgacgat catcgacaag gccgtcgcga
     2041 cgatcaatcg cgcacgcgcg agccgcagcg agccgccgct gaacgtcgat gcgatccccg
     2101 tcgacgacgc gaagacctat gagctgttgc ggcaatgccg cacgaccgcg gtgttccagc
     2161 tcgagtcgct cggcatgcgc gatctcatca agcgcttgca gccggaccgg ttcgacgatt
     2221 tgacggccat cgtggcgctg tttcggccgg ggccgatgca gatggccgac gagttcatcg
     2281 agcgtaagca cggcacgggg ggggcgcgtg actttttgca tccgcggctc gagacgacgc
     2341 tgaagccgac ctacggcgtc atcctctacc aagagcaggt catgcagatc gcgcaaattt
     2401 tggccggcta ctcgctcggc ggcgccgatt tgctgcggcg cgccatgggc aagaaaaagc
     2461 ccgaagagat ggcgcagcag cgcgccgtat ttttgaaggg ggcgacggag cgcggtgtcg
     2521 aggagtcgcg cgcgaatcac atcttcgatc agatggagac gttcgcgggc tacggcttca
     2581 acaagtcgca tgccgccgcg tacgcgttga tcgcctatca aaccgcgtgg ctgaagacgc
     2641 attaccccga ggactacatg gccgccgtgc tgacggccga ttctgacaac accgatcggc
     2701 tcgtcgtcct caaggacgag ctgaagcgta tgggcatcgc gctcgagccg ccgaacgtca
     2761 acgcgtcggt gctgccgttc acggtcgccg gcccgaagcg aatcagttat ggcttgggcg
     2821 cgctcaaagg cgtcggcccg agcgccgtgg aggcgatcgt ggcggagcgc gcgcacggcc
     2881 cgtacaagag cctcgtggat ctctgtcgcc gcgtggattt gcaaaagatc aaccggcgcg
     2941 tgctcgaagc gctcgcgaaa tcgggcggcc tcgattcgct cggcgcgaac cgcgcgacgc
     3001 tgatgaacgg catctcgaac gccctgcaag cggccgagcg gtccgtgcac gcgcaagccg
     3061 ccggccagac ggcgttgttt ggcggcgacg agcgcgacga cacgctcgag cacgtgctga
     3121 cgcccgtccg cgaatggagc aagcgcgagc ggctcgaggg cgagcgcgag agcctcggcc
     3181 tctacttgac gggccatccg ttcgacgact tcgcggagca ctgcaagcac ttcacgaacg
     3241 gcgcgatcgc gaaagttctg caggggctgc caagctcggc gctgccgtac cacgtgcgca
     3301 aggaagcgat cctcgccggc gtcgtcgtcg acgtccgccg ccgcggcaat cgcgtctcga
     3361 tcatcctcga cgacgacacg gagcgcatcg aggtcacgat gttcgacgag gtgttcgcac
     3421 agtcgaagca cgtcatcgcg aagcacgccg tgttgatcgc cgaagggcag ctgcgctacg
     3481 acgacttcat caacggctgg cggctcacgg cgaagcgcgt gcgctcggcc gacgaggcga
     3541 tcgaggagta cgcgcggcgg ctcacgattc gctggccgag cgacggcgtc ggcacggagc
     3601 tcgtgcgcga gctgcagcgg attctgaagc cgttcacgcg cgggcgctgc gaggtgtcga
     3661 tcgagtaccg gcgcaacggc gccgaggcga cactgacgct cggcgaagcg tggtcggtcc
     3721 gctgcacgcg cgagctgcgc gaccaactca cacgcctcct cggcgacgac cgcctcctcg
     3781 tccactaccc gaagcacttc gtctaacgct gcgcaccgtg accgttgccg gggagcgcac
     3841 ggcgggctcg acgcagtgtt ccgcccttcg cccgttggcc cgatgctttc gagtcgcacg
     3901 tgagagcgcg cgcgggccgg gcgtgcctgc gaagctttgc accgcgccat ggagcgatcg
     3961 ccatcgcggg ggtcgtctcg ggttccttgg cccgctgacg tgcgggccac cgagctcaga
     4021 acgaaacact gcgccgaacc cgccctccag cgcggcgaag aacagcgcgt cgcaggtctc
     4081 gaccggcggg gtcgatggag tgtttcgtgc tgagcgcggc ggctccgcag gcaagcgggg
     4141 caatcgacct gcgatgaggc tcatggtggc agatgcgtcg ccctgcgttg caaagcttcg
     4201 aaggcactcg cggcccgcgc cttctctcac gtgcgctgcg acagcatcgg gccgccgcgg
     4261 gaagcgcgga acactccgtc gggcccgccg caccaccgag cgtaaatggt cacgcatgcg
     4321 gcgccgcgct ccgtgcttgt aagggtgaag gggggtcggg taaggtgcga gcgtcgcggt
     4381 cgcccgaggg gctatggacc tgaaattcct ggagtttgaa caaccgatcg ctgagctcga
     4441 ggcgcgcatc gacgccctca agttcgtcgg tgaggactcg gagctcaaca ttagcgaaga
     4501 aatttcacgc ctcaggaaca agagcgagtc gctgacccgc agcatcttct ccaatctcac
     4561 ggcctggcag atcgcgcagc ttgcgcgtca cccgcaacgg ccgtacacgc tcgactacgt
     4621 cgacaagtgc ttcacggatt ttcaggagct gcacggcgat cgcatgtacg ccgacgacca
     4681 ctccctcgtc ggcgggttgg cgaggctcga caacgagccc gtcgtcgtca tcggccacca
     4741 aaaagggcgc gacacgaaag agcgcgtgtg gcgcaactac ggcatggcgc ggcccgaggg
     4801 ctatcgcaag ggcttgcgcc tcatgcacct cgccgaaaaa ttcggcttgc cgctgatcac
     4861 gctgatcgat acgccgggcg cgtaccccgg catcggcgcc gaggagcgag ggcagagcga
     4921 agccattgcg cgcaatctgt tcgtcatgtc gagcctccgt gtgccgatcg tcagcgtcgt
     4981 gatcggcgaa ggcggctccg gcggcgcgct tgcgatcggc gttgcggacc gcgtgctgat
     5041 gttgcagtac agcatctact ccgtgatctc gcccgaaggc tgcgccagca tcttgtggaa
     5101 gagcgccgac aaggcggagc tggccgccga agcgatgggt atcacggccg aacggttgca
     5161 ccgcttgaag ctcgtcgatg aggtcatcgc ggagccgctc ggtggcgcgc atcgcgaccc
     5221 gcaggtgacg gccgacgcgc tgaagcacgc attggcgcag acgctcgcga cgctgcaagc
     5281 catcccgctc gacagcctgc tcgtgcagcg gcgcaagcgg ctggccagct acggagtctt
     5341 caaggaaagc tgacctccgg cgtcgccgga agtccgcggc gcagcatgac gacacctccg
     5401 ccaccgcagg ccgacgacgt gacggccgtc gtccgcgcga cgctcgagcg ttgggcttcg
     5461 cgcgagctcc cgccgcgtat caccgtcgcc ttcagcggcg ggctcgactc cacggtgttg
     5521 ctgggcgcgc tgtgtcggct tgcgttgccg gtgcgtatcc gcgctgcgca catcgatcat
     5581 ggcttgcagc cgcagtcggc cgagtggagc gagcattgcg cggcgacggc cgcggcgctc
     5641 ggcgcgcagt tcgtgacggc gcgcgtcgcc gtcgaccaga ccgcggggct cgggctcgag
     5701 gcggcggcgc gcgacgtgcg ctatcgcacg ctcggcgagc tcatgctggc cggcgaatgg
     5761 ctgttgacgg cgcatcacgc ggacgatcag ctcgagacgt tgctgctgcg cttcatgcgc
     5821 ggcaccggcg tgcgcgggct gcgcggcatc atcccgttcg ggccgttcgg cgtcggcgca
     5881 ctgggccggc cgctgctctc gttcacgcgc gcgcagctgc acgctcgagc cctagagtgg
     5941 aatctgcgct ggctcgagga cccttcgaac cgcgagccgc gtcaggaccg caattacctg
     6001 cgcttgcacg tcgtgccggc gctgctcgcg cgctggccgg acgcggcgca gcacgccgag
     6061 cgtctcgcgg agcaggccgg cgaggccgag cggctgctcg acgccgtggc ggccgaggac
     6121 gcccggccgc tcgtcgcgcc gtggcatgtc ccgcgcaccg tgctggccgc gctcgagccg
     6181 gcgcggcagc gcaatttgtt gcgccacgtg ctgcgcgccg taggcctcgg gacgccaagc
     6241 gcgcgcaagc tcgaggaact gcgcgccgcg ctgctcgaag cgcacgcgga ttcgcatacg
     6301 caagtaagtt ggccgggtgc cgaaggccgc gtctttcgcg acgcgcttta tctcggcgtg
     6361 ccgcccgccg cgcactcgcc ggcggaccat gccgcgcgca tcgatagcgc ggccgcgtgg
     6421 agcgggcccg agggccgcgt cgagctgctg cccgtgcaga cgagcccagg ggtgccggag
     6481 tcgtggctcg cagaaggctt gacgctgcgc tttcgtgccg gcggcgagcg ctttcggccg
     6541 cgcggccggc agcatcacca ctcgctgaaa gatttatttc aggaacacgg cgtcgtgccg
     6601 tggatgcggg accgcgtccc gttgctctac cgcggcgctg cgctcgtcgc aatcggcgac
     6661 ctctggctca gcgccgacgt cgacgccgcg ccggcgaccg agccgcgcta cagcgtgcgg
     6721 tggaccgagc atccgccggt gcgcgcgccg gaaaagagct gagcgcccgc gcgcgatgcg
     6781 gggctggtcg ggccgaggta cttttggtac tttgctctcc cgtctcgatg tatctaacac
     6841 tcaacttcga tggccggacg aatgcttccg ttggaagcgg cgttcggccc tcccggtttt
     6901 gacatgacac gattcatctt catcaccggc ggggtcgttt cctcgctcgg caaaggcatt
     6961 acctcggcct gcctcggcgc gatcctcgag gcgcgcgggc tcaaggtgcg cctcatgaag
     7021 ctggatccgt acatcaacgt cgacccgggg acgatgagcc cgttccagca cggcgaggtc
     7081 tacgtcacgg acgacggcac cgagacggac ctcgatctcg gccactacga gcggttcgtg
     7141 cacacgcgca cgaaccgcat gagcaacttc acgacgggca agatctactc gaacgtcatc
     7201 gcgaaggagc gccgcggcga gtatctcggc gccacggtgc aggtgattcc gcacatcacc
     7261 gacgagatca agcgcaacgt gatcgcgggg gcgggcgacg cggacgtctg catggtcgag
     7321 atcggcggca ccgtcggcga catcgagtcg ttgccgtttc tcgaggccat tcgccagatg
     7381 ggcgtcgagc taggccgcga tagagccctc tatatccacc tgacgctcgt gccgttcatg
     7441 gccgcgtcgt ccgagatcaa aacgaagccg acgcagcact cggtcaagga gctgcggggc
     7501 atcggcatcc agcccgacgt gctcgtgtgc cgttccgagc acgcgttgcc gcgcgagcaa
     7561 cgcgccaaga tcgcgctgtt cacgaacgtc gaggagcgcg ccgtcatctc ggccgtcgac
     7621 gtcgacgaca tctacaaaat tcccatgctg ctcgccgagc aggggcttga tagctacgtc
     7681 gtcgacaagc tgaggctcga cgcccgcccg acggatctat cggagtggca ggccgtcgtc
     7741 gacgccaaag cgcacccaga ggccgaggtc accgtc
//
LOCUS       DATLEE010000716         3485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8830856, whole genome shotgun sequence.
ACCESSION   DATLEE010000716 DATLEE010000000
VERSION     DATLEE010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8830856"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /gene="galE"
                     /locus_tag="VM692_15665"
     CDS             <1..762
                     /gene="galE"
                     /locus_tag="VM692_15665"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HUQ53665.1"
                     /translation="VVPESVANPLKYYGNNTCASRTLLEHALKAGVKHVVFSSTAAVY
                     GIPGDGEASEETPTAPINAYGTSKLMTEWMLRDVAAVNPLSYVALRYFNVAGSDPSGR
                     IGQSTPKATLLTKVACEVVVGKRPYVSIFGTDYPTPDGTGVRDYIHVEDLADAHLKAL
                     DYLRRGGESTTLNCGYGRGYSVRELLAMVERVAGHPIAKKEEPRRAGDPPTLVARAAR
                     IRATLGWQPRHDDLAKIVATSLEWERKLAAGHWRS"
     gene            782..2260
                     /locus_tag="VM692_15670"
     CDS             782..2260
                     /locus_tag="VM692_15670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUQ53666.1"
                     /translation="MKIRRPRTLLGMVLMGLAFVTVPLLVAVGNAMIQLGQLAAESEV
                     VLGDMRTVTLENQKLANSLERMERSVRQFFLLQNVTTSSDSQSLYDGDQAAFESSIAA
                     LRALPSDVSIRDQLERLSSISKEVHRAFRVGRTDGAGDAIIARFSVLTEATRAVATAM
                     RDAINQRLEELQESTRSAQQELVWLSAALIPGILVLVGLFLLLVGRPMRQIDRAIREL
                     GEGDFSRPITVSGPHDIETLGRQLEWLRHRLKESTDEKNKFLRHMSHELKTPLANIRE
                     GTELLLDGSVGQLDIQQQEVTSILRDNGVKLQKLIENLLTFSAWQTHTAALELSEFEL
                     KPMVFSVLSQHRLVLSNKKIKLQLAISAINVLADEGKLKLVLENLLSNAIKFTPNGGT
                     IHVAAGMHGHDLVMDVSDDGPGVHPDDGDRIFEAFYQGRRPQGGPVGGTGIGLSVVAE
                     CVQAHGGAVELVRGDEASGAHFRVRLPLRRAADKRLRAVANA"
     gene            2253..2873
                     /locus_tag="VM692_15675"
     CDS             2253..2873
                     /locus_tag="VM692_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53667.1"
                     /translation="MRKAVPVLLVCVVAGCTSLGDSPDDRPATNVTAPSPRPAADVGD
                     SLAVYLQTLRSLIEGDPVVQADVFRNVAAQADAAPTTTNRLMLALALATPTHPSSDAA
                     AAQRLLSDLLARGDALLPEEQVLALIHLKDVEQRLILDAEAERLQRAAAAATAQRNDR
                     SAQQLQAALEENRQLKAALDEARAKLDAITNIERSIRERENAPDSQ"
     gene            2854..>3485
                     /locus_tag="VM692_15680"
     CDS             2854..>3485
                     /locus_tag="VM692_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUQ53668.1"
                     /translation="MHQIHSKPPGQAPLRGRILLVDDDPGLLRLLSIRLRAEHYDVEA
                     VESAAEALGALSRFRPDLVVTDLRMDNMDGIQLLKEIQRQRPGLCVLLLTAHGTIPDA
                     VEATQSGAFGFLTKPVDKQQLLEYVERAMKVSGMPKSTDEWSAEIITRSPAMEACLQQ
                     ARMVANTDTRVLITGESGTGKELLARAIHRASTRKDQPFVAINCSAMAENL"
BASE COUNT          659 a         1137 c         1145 g          544 t
ORIGIN      
        1 gtggtcccgg aatccgttgc caacccgctg aaatactacg gcaacaacac gtgcgcgagc
       61 cgcacgttgc tcgagcacgc gctcaaggcc ggggtgaagc atgtcgtgtt ctcctcgacg
      121 gccgccgtct atggcatccc cggcgacggc gaggcctcgg aagagacgcc cacggccccg
      181 atcaacgcct acggcacgtc gaagctgatg acggaatgga tgctgcgcga cgtggccgcc
      241 gtcaatccgt tgagctacgt cgcgttgcgt tatttcaacg tcgcgggctc ggacccgagc
      301 ggcagaatcg gccagtccac gccgaaagcg acgctactga cgaaggtggc ctgcgaagtt
      361 gtggtgggca agcgaccgta tgtgtcgatc ttcggcacgg actatccgac tcccgacggt
      421 accggtgttc gggactacat ccatgtcgag gacttggcgg acgctcattt aaaggcgttg
      481 gactacttgc gccgcggcgg cgaatccacg acattgaact gcggctacgg ccgggggtat
      541 agcgttcgcg agttgctcgc catggtcgag cgtgtcgcgg ggcacccaat agctaaaaaa
      601 gaggaacccc ggcgcgccgg cgatccaccc acgttggtcg cccgcgccgc tcgaattcgt
      661 gccacgctcg gctggcagcc gcggcacgat gacctcgcca aaatcgtagc aacgtcgctg
      721 gaatgggaac gaaaactcgc tgcagggcat tggcgctcgt gatctttcgg ggcgcaaacg
      781 aatgaagatc cggcggccgc ggacgctgct cggcatggtg ttgatgggcc tagcgttcgt
      841 caccgtgccc ctgctcgtcg ccgtcggcaa cgccatgatc caactcggcc agcttgcggc
      901 ggaaagcgaa gtcgtgctcg gcgacatgcg cacggtgacg ctcgagaacc agaaactcgc
      961 gaattcgctc gagcgcatgg agcgcagcgt tcgacagttt ttcctgctgc agaacgtcac
     1021 gacgtcgtcg gactcccagt cgctgtacga cggcgatcaa gccgcattcg agagcagcat
     1081 cgcggcgttg cgggcgctgc cgagcgacgt gagcatccgc gatcagctcg agcgtttgtc
     1141 gtcgatctcg aaagaagtgc accgcgcgtt ccgcgtcggc aggaccgacg gcgcgggcga
     1201 cgcgatcatc gcgcgcttca gcgtgctgac cgaggcgacg cgtgccgttg cgacggcaat
     1261 gcgcgacgcg atcaatcagc ggctcgagga actgcaagag agcacgcgtt cggcgcagca
     1321 ggagctcgtc tggctgtcgg ccgcactgat acccggcatc ctggtgctcg tcggcctgtt
     1381 cttgttgctc gtcggacggc cgatgcggca gatcgaccga gcgatccgcg agctcggcga
     1441 aggagacttc agccggccga tcacggtcag cggaccgcac gacatcgaga cgctcgggcg
     1501 tcagctcgaa tggctgcgtc atcggctgaa agagtcgacg gatgagaaga ataagttcct
     1561 gcgccatatg tcgcacgagc tcaagacgcc gctcgccaac attcgcgagg gcaccgagct
     1621 gctgctcgac ggctccgtcg gccagctcga cattcagcag caggaagtca cgagcatcct
     1681 gcgcgacaac ggcgtcaagc tgcagaagct catcgagaac ctgctgacgt tcagcgcctg
     1741 gcaaacgcac acggccgccc tcgagctgtc ggagttcgag ctgaagccga tggtgttcag
     1801 cgtgctgagc cagcatcggc tggtcctctc gaacaagaag atcaagctgc aactggcgat
     1861 cagcgcgatc aacgtgttgg ccgacgaagg caagctcaag ctcgtgctcg agaacctgct
     1921 gtcgaacgcc atcaagttca cgccgaacgg cggcacgatt catgtcgccg cggggatgca
     1981 cggtcacgat ctcgtcatgg acgtcagcga cgacggcccg ggcgttcatc ccgacgacgg
     2041 cgatcgaatc ttcgaggcgt tctatcaggg ccggcggccg cagggcggcc ccgtcggcgg
     2101 caccggcatc ggcctatccg tcgtcgccga gtgcgtccag gcgcacggcg gcgcggtcga
     2161 gctcgtgcgc ggcgacgaag cctccggcgc acattttcgc gtgcggctgc cgctgcgccg
     2221 agccgccgac aaacgcttgc gggcggtggc caatgcgtaa agcagtgccg gtactgctcg
     2281 tctgcgtggt ggccggctgc acctcgctcg gcgactcgcc agacgatcgc ccggccacga
     2341 acgtgacggc gccttccccg cgtccggcgg ccgacgtcgg cgacagtctc gcggtgtatc
     2401 tgcagacgct gcgcagcctc atcgagggcg atccggtcgt gcaggccgac gtgttccgga
     2461 acgtcgcggc gcaagccgac gcggcgccga cgacgacgaa ccgcctcatg ctcgcattgg
     2521 cgctcgcgac gccgacgcat ccgtcgagcg atgccgcggc agcgcagcga ctgttgagcg
     2581 acctgctggc cagaggcgac gcgctgctgc ccgaggagca ggtcctcgcg ctgattcatc
     2641 tgaaggacgt cgagcaacgg ctgatcctcg acgccgaggc cgaacgcctg cagcgcgcag
     2701 ccgcggccgc gacggcgcaa cgcaacgatc gcagcgcgca acaactgcaa gccgcgctcg
     2761 aggagaaccg tcagctaaaa gccgcgctcg acgaggcgcg cgccaagctc gacgccatca
     2821 ctaatatcga acgatcgatt cgggaacgcg aaaatgcacc agattcacag taagccgccc
     2881 ggtcaggcac ctttgcgagg tcgcatcctg ctcgtcgacg acgacccggg tctgctgcgc
     2941 cttctgagca tccgcctgcg ggccgagcac tacgacgtcg aagccgtcga gagcgccgcc
     3001 gaagcgctcg gcgcgttatc gcgattccgg cccgacctcg tcgtaaccga tttgcgcatg
     3061 gataacatgg acggcatcca gctgctgaaa gaaatccagc ggcagcggcc cgggctctgc
     3121 gtgctgttgc tgacggcgca cggcacgatc cccgacgcgg tcgaagcgac gcagagcggc
     3181 gccttcggct tcctgacgaa acccgtcgac aagcagcagc tgctcgagta cgtcgagcgc
     3241 gccatgaaag tgtcgggcat gccgaagagc accgacgaat ggagcgccga gatcatcacg
     3301 cgcagccccg cgatggaagc gtgcttgcag caagcgcgca tggtcgcgaa cacggatacg
     3361 cgcgtgctga tcaccggcga gtccggcacg ggcaaggagc tgctcgcgcg cgcaatccat
     3421 cgcgcgagca ctcgtaagga ccagccgttc gtcgcgatca actgcagtgc gatggcggag
     3481 aacct
//
LOCUS       DATLEE010000717        14300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7341077, whole genome shotgun sequence.
ACCESSION   DATLEE010000717 DATLEE010000000
VERSION     DATLEE010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 14300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14300
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7341077"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VM692_15685"
     CDS             complement(<1..613)
                     /locus_tag="VM692_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUQ53669.1"
                     /translation="MNDEVLVWRGHRVRVSQAGAGDPLLLIGGLGSHLGMWAPFAAEF
                     RERRIIRFDMPGSGLSSTPLYPISIAMLAELAVAVLDHCSTPQADVVGISYGGAVAQQ
                     TAHDHPQRIRRLVLAATTCGVGAVSGSIAALTALATPLRFYSPSYFERVAAAVYGGAT
                     GRDASARKEQLRIRSHAPPPSSYGYAMQILGGYAWSSLPFLSLI"
     gene            803..1909
                     /locus_tag="VM692_15690"
     CDS             803..1909
                     /locus_tag="VM692_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUQ53670.1"
                     /translation="MHTNVDRTALIDDPPPTTPFEVVDECGLLRLRHYGPPRAASAAP
                     VLFVYSLFKRPYVLDLLPERSLVQTFLREGFAVYVTDWLPPLSQDGVRGLHDYVNCDL
                     AHAVECVRKREGAERISLVGCCLGGVLAVIYAALHPHTVERLVPFALPFDSRPPFPPG
                     AAAQLVSLYGNVPSWWIRMALNSRVSNPFRLPSFLAEELGEPELEECDGGQRTDVQVR
                     LEQWFGSDVPLAGRLFCEVMQGAFGDSQFAADRLQVGDRCVQLADIECPVLNISAERD
                     LLVPPQDSAAFVERVGSREASNLVFPNGHLGLMVSRAAHETLWPYVASWLRGGVAERQ
                     PGIPAPFGSAELLGSTKSARSRAERGASARRGQS"
     gene            1990..2310
                     /locus_tag="VM692_15695"
     CDS             1990..2310
                     /locus_tag="VM692_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53671.1"
                     /translation="MSTYGRLAAEATDQYLAALDTAQQNFLKLFATMPAPSLSYQPST
                     MPGLPTAQEIIEASFSFTEKLLVQQQSFMQKLVASPEPRTEVPAAPAVAMGKAVSPKS
                     KNAN"
     gene            complement(2368..3663)
                     /locus_tag="VM692_15700"
     CDS             complement(2368..3663)
                     /locus_tag="VM692_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ53672.1"
                     /translation="MNQLRLALAVVAALVTAPAAFAQHTSADTSASALHWAAHNNDVG
                     AVKRLLSEGADPNLANRFGLTPLHEAATARNAEMLELMLDAGGDANATFGEGETVLMT
                     AARAGDLASVRALLAHGGLPDATENWHGQTALMWAAIENHADVVAALIEAGAEVDRAS
                     TKHDWVKISYSEGNVPKTRDLGGLTALQFAARQGSAQAVETLLDAGADASKSEPQYQL
                     TPLQLAIVNGHYTLAKRLVERGVGVDDGSLYVVVDTRNLGFYAQRPNPPEKDGDVTSL
                     DLVNALLEHGANPDLPYTKGEIDGDAKPRLPERTVAGEILVPPGATPLDRAAAAGDFA
                     VVETLLAHGASATVVTEDGTTPLMLLAGYSRARGNAPLTDVPKRLALIRTLLEKGADV
                     HAVHKDSTNNALYFAKQRNAAEIVALLEQFGAHEATAAN"
     gene            complement(3683..5047)
                     /locus_tag="VM692_15705"
     CDS             complement(3683..5047)
                     /locus_tag="VM692_15705"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ53673.1"
                     /translation="MFISKKQLSRRTILRGVGASLALPLLDAMTPAFAQAPARTLRFG
                     TIYIPHGAIMDVWMPKKTGADFELPIILRPLGGLREHFNVVTGLYSEGANAHSACPGF
                     FSACAHAPRGNLVELDTSIDQVIAQKIGQGTTFPSMELAIEDSSNIFGTCAADFLCTY
                     MDTISWRTPTQPLPMELNPRVVFERMFGGDGTSAEARRARLEQTTSILDAIVEDVHGL
                     AGKLGARDRARLDEYLSNVREVERRIVQAEDQRSERRLEAPPTPSGVPESFEEHVNLM
                     FELQALAFQGDLTRVTSFMMGRELSGLSYPQIGISDGHHPISHNNYDAKQMEKKAKVD
                     TYNVSLFAKFLQRLKDTPDGDSNLLENSLFVYGSGMSDGNVHNHLNLPILLAGNAGGR
                     LKGGRHIQAGQLAAERAIPAIPKFDKMVPLANLMVSVLELYGIEAESYGKAMCASNGR
                     VSLA"
     gene            complement(5061..7463)
                     /locus_tag="VM692_15710"
     CDS             complement(5061..7463)
                     /locus_tag="VM692_15710"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ53674.1"
                     /translation="MRVPVVIALLALASAACQPSDEQRAAAAKDVIERYCTDCHNDAE
                     RTGELSLEGLALTDVGAHAEVWETVVKKLRGRMMPPAGEPLPEAATANELVAFLETRL
                     DAAAAENPNPGLKSLHRLNRTEYGNAIRDLLALEVNATALLPNDSEAYGFDNIADVLG
                     TDPSLMDRYLSAAWKVTSAAIGDTDIAPAVSTYRVPPDRSQTDYVEGLPLGTRGGMLV
                     SHHFPVDGEYVIRPRLWRNTVDVVRGTETPHDLEVSLDGARLSLTRFGGPDDEVPAQM
                     FPGKTADEIDRRFETRVTVAAGQHDVGVAFAKKSSATRQEVLQPFAREKHDARMDVGI
                     PELDQIVIEGPLSIAGPGHSPSRARIFSCYPAAAAEEAPCATDILSTLARRAYRRPIT
                     DAERERLVGLYQAERAKGRDFEAGVQTGLAYILVAPQFLFRVEPDPADAAPGTLYKIS
                     DLELATRLSFFLWSSLPDDELVDAAVAGNLSKPAELRKQVERMLADERATTLAKSFAS
                     QWLYLRNLRASSPDMYVFPDFDDNLRQSAIRETELLFETIVREDRPLTELLSADYTYL
                     NERLARHYGIPSIYGDQFRRVTLTDTRRFGLLGQASILTLSSYPNRTSPVSRGQYVLT
                     NILGTPPPEPPPDVPTLPENTTEKLSMRARMERHRADPVCAGCHKLMDPIGLVLERYD
                     GIGRWRSEDNGEPVVDYGSPIHVLRDFGPINGPEDLRAAILSQPERFVRTGTEKLMTY
                     ALGRALTAADMPTARAIVRQAADADYRFSSLVLGVVTSEPFQMRIAGTDADTKVALDS
                     SR"
     gene            complement(7523..8659)
                     /locus_tag="VM692_15715"
     CDS             complement(7523..8659)
                     /locus_tag="VM692_15715"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53675.1"
                     /translation="MPSRHRYAAAPALALAAAAPALAHHGFGLFQMQEFKDYSGTLTK
                     LELVNPHSYLYFETFGADGKPLEMRCEMRAATLIRRSGWKTDEFVPGRHVDIHAHPHR
                     DDPHSCYLESVAIGGTTTIDRNDQFTHAPVDTSKRPGRLSSGEPNISGDWAVEQAVLT
                     IPPSGGNGSLVPRSLREDFAAGKITLEQIRALNPQPSRPQYTAKGQEAATAFRMWSTE
                     DNPRLSCKPTSIIFDWTFDWPVNRITQTTMGGEKVIDIDYGLFSATRRIHVGMDAHPA
                     NLAPSNSGHSIGRWDGDTLVVDTVGFAEGVLVPPTRNSPQLHVVERFTLDPQTLALKR
                     EYTAEDPVYLTAPYKGQDVVLLSETPFEKQPCSELTFEFTQPGN"
     gene            complement(8829..9875)
                     /locus_tag="VM692_15720"
     CDS             complement(8829..9875)
                     /locus_tag="VM692_15720"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HUQ53676.1"
                     /translation="MNNNSCTWIALALVLGSPAVALAQALPNPYRLVDNWAKLPSGRE
                     IGAVGDVDIDVDGRHVWAVLRCDAGGDKFGYECLESDLDVVIKFAPDGNAVESFGGGL
                     FIWPHGLEVDPQGNVWVTDAADEQRTNGPNGRGRGHQVIKFSPRGEVLLRLGTAGVAG
                     NDRTHFNAPSDIVVAPNGDIFVADGHGDATNNRVVKFAADGRYLKEWGHTGYGPGEFR
                     TMHAIAIDAQGRVFVGDRGNNRIQLFDQEGTYLTSWTQFGRPSGIFFDHLGRIIVADS
                     ESDDIENPGWDMGIRIGDAASGAVREFILYPWGDPRVRAGTGAEFAVVDRDGNIYGGE
                     PRPRRLQKYVRVRP"
     gene            complement(9872..10594)
                     /locus_tag="VM692_15725"
     CDS             complement(9872..10594)
                     /locus_tag="VM692_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HUQ53677.1"
                     /translation="MRRLMALVLVSIVTLAGVGGERACTPQERLAGRPKLIDGDSFEL
                     GNVGVRLFGVDAPEGRQSCTRDGRDWSCGDSAAAELRRLVGSRDVACEQRDKDTYGRI
                     VAVCRVGTTDLGEAMVRAGMATAYRRYSNDYVDEENEARRARRGIWAGEFTPPETYRN
                     EQRDTPRERAAAPPPQSQRDGCYIKGNINGDGERIYHTPGSSSYADTAIDESKGERWF
                     CTEGEARTAGWRAPRQPKGGSR"
     gene            10677..11807
                     /locus_tag="VM692_15730"
     CDS             10677..11807
                     /locus_tag="VM692_15730"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009605534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ53678.1"
                     /translation="MAKRVTLLTCAIAAAFAACGDFGGSFAQSPFTTTPVAEFNEPWA
                     MAFLPDGRLLVTEKRGALKLHSLETKRTGDVAGVPAVAYAGQGGLGDIVLHPNFTTNG
                     LVYLSYAEAGERALAGAAVARARLVLDKSTGRLENLAVIWRQTPKVDGRGHYGHRIAF
                     GPDGFLYISSGERQKFTPAQDLQQNLGKIVRLSDDGSVPADNPFGERGGVTAQIWSLG
                     HRNPLGLAFDAQGQLWNVEMGPMGGDELNRVQRGANYGYPLVSNGSHYGGADIPDHDT
                     RPDLAAPAVFWNPVISPSSLLFYSGSEFPDWQGDALIGGLSSTSIVRIEFGAGGTARE
                     AARYRMPQRMRALEQGPDGSIWALEDGRGGRLLKLMAAAQSR"
     gene            11862..12899
                     /locus_tag="VM692_15735"
     CDS             11862..12899
                     /locus_tag="VM692_15735"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53679.1"
                     /translation="MRSSHFVTFVAGLAAAGLAGLAAAHHSQVGIFDSKENIEVTGVI
                     KSISWRNPHGQILLTQTNPDGTTTEWDAETASIAVMRNRGTEPSAVVVGDRVTIAGAP
                     SVRGRKEILARSMLLSSGYEFTFGGAQAYFPEGKSGRIVGRATIEADVKEATAKADGI
                     FRVWSTIMSDPAAFPMFKGGYPLTAAGRAGLAQWNPRDNSLLKCGTKGTPLIMITPLP
                     VEFVRDGDKITMRIEEYDTVRTIHMNANAAAPAEHTLLGFSRGRFEGNTLIVETDHIK
                     PGYFDHEGTPMSDQIKVVERFTPNADYTRLDYKLTTTDPVNFERPFDLTRYFIWKPEN
                     TVHPYECLDRF"
     gene            complement(12918..13334)
                     /locus_tag="VM692_15740"
     CDS             complement(12918..13334)
                     /locus_tag="VM692_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53680.1"
                     /translation="MRDAQFESLRLELLQRGVTPVYVERTILELREHYDDLESAARAS
                     GLSAEEATRTARATLGDERVIAAAVLAHSELLTFSTRWPRIAYCLHSAATVPGLPLLY
                     CIEHRPELARWGVAFGAATTLMGSVLAALNWLIVLV"
     gene            complement(13327..13677)
                     /locus_tag="VM692_15745"
     CDS             complement(13327..13677)
                     /locus_tag="VM692_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HUQ53681.1"
                     /translation="MSQTNPPFLTGVPELLVLRMLSGREMYGYEIVKAMRLVTAEAIG
                     FGEGVIYPVLHSLEEGGALKARRKSVNGRERVYYSVTTKGKRRLEHLSDEWKRVSSGV
                     NSALNGAHGHPGHA"
     gene            complement(13739..>14300)
                     /locus_tag="VM692_15750"
     CDS             complement(13739..>14300)
                     /locus_tag="VM692_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HUQ53682.1"
                     /translation="AGAVLSVGLFLGLWSLVSVPFDAPPIEKARIIKFTADRVMTPIV
                     PRRPTKLQPPPAQTLVINPRIRGLPTEPIRPIPQQLTEVARPPRTGMPMGADRDVTPR
                     VRVNPDYPPSAARNGTEGWVQVRFTITEAGTVRDAVVVRSEPSRVFDEAALKAIARWR
                     YNPRVDAGVAVERVGIETLIRFTLEN"
BASE COUNT         2307 a         4642 c         4820 g         2531 t
ORIGIN      
        1 ggatgaggct taagaaaggc aggctggacc atgcgtaacc gccaagaatt tgcattgcgt
       61 agccatacga agacggcggc ggcgcatggc tgcgaatgcg cagttgctcc ttgcgcgcac
      121 ttgcgtcccg accggtggca ccgccgtaca ctgccgccgc gacgcgttcg aagtacgagg
      181 gcgagtaaaa ccgcagcggg gtggcgagcg ccgtcagggc ggcgatcgag cctgaaaccg
      241 cgccgacccc acaggtcgta gctgcaagca cgaggcgccg gatccgctgc gggtggtcat
      301 gagcagtttg ctgcgccacg gcgccgccat aagagattcc tacgacatcc gcttgtggtg
      361 tagagcaatg atcgaggacg gcgacggcga gctccgcgag cattgcaatg gagatcgggt
      421 aaagcggtgt gctcgatagc cccgagccgg gcatgtcgaa gcggatgatt cgacgctcgc
      481 ggaattcagc ggcaaacggt gcccacatgc cgagatggct gccgagaccg ccgatcaaca
      541 acaacgggtc gccggcccca gcctgcgaga cgcggacgcg atgaccccgc caaaccaata
      601 cttcgtcgtt caatttgctg cacctcttgt ctcaggtgca atttgcatgc cgtgtctcgc
      661 aagcggccac ggtatcggtc gggtcgacat cgcgaattaa cgggcgctaa atgcgaggcg
      721 gcaatacgag aaatccaaat ccacaaaacg cgagtggtcg tagcggcacg ccgagcgcga
      781 taccgagacc gccggagcac ggatgcatac gaacgttgat cgaaccgccc tcatcgacga
      841 tccgccgcct acgacgccat tcgaagtcgt cgacgaatgc gggctattgc ggctgcgcca
      901 ctatggcccc ccgcgtgcgg cgagcgcggc gcccgtcctt ttcgtctact cgttgttcaa
      961 gcggccctac gtgctcgacc tccttcccga gcgcagcctt gtgcagacct ttctgcgtga
     1021 agggtttgcc gtttacgtga ccgactggtt gccgccgctt tcccaggacg gcgtgcgcgg
     1081 cttgcacgac tacgtcaact gcgatctcgc acacgcggtc gagtgcgtac gaaagcgcga
     1141 aggcgcggag cgaatctcgc tggttggatg ttgcctcggc ggcgttctcg cggtgattta
     1201 cgctgcgttg catccccaca ccgtcgagcg cttggtcccg ttcgctttgc catttgactc
     1261 gcgaccgccg tttccgcccg gtgccgctgc gcagctcgtg agtctttacg gcaacgttcc
     1321 ctcgtggtgg atacgcatgg cgctgaacag ccgcgtgtcg aatccgttcc ggctgccgtc
     1381 ctttctcgca gaagagctcg gcgaaccgga gctcgaagag tgcgacggag gccagcggac
     1441 cgacgttcaa gttcggctcg agcagtggtt cgggagcgac gtgccgctgg cgggacgcct
     1501 gttctgcgag gtgatgcagg gggcattcgg agattcgcaa ttcgcggcgg accgcctgca
     1561 ggtcggcgat cgatgcgtgc agctcgcgga catcgagtgt ccggtgctca acatcagcgc
     1621 cgagcgcgat ctgctcgtgc cgccgcaaga cagcgctgcg ttcgtcgagc gcgtcggcag
     1681 ccgcgaggcg agcaacctcg tttttccgaa cggccacctg ggattaatgg tcagccgcgc
     1741 ggcacacgaa actctctggc catacgtcgc gtcgtggctt cgcgggggcg tcgcagagcg
     1801 tcagccagga attccagcac cgttcggctc agccgagttg ctcggctcga cgaagtctgc
     1861 gcgtagtcgc gccgagcgcg gcgcgtcggc acggcgaggt caatcgtaag aagcctgcgc
     1921 ttggtactct gcgcaaatgc gttgtatact gcgcattccc ccaaggatga atacgcaagg
     1981 agcaactcaa tgtcgaccta tggaaggctc gccgccgaag ctacagatca gtatctggcg
     2041 gcgctcgata ccgcccaaca gaatttcttg aagctattcg ccacgatgcc cgcgccgtcg
     2101 ctgtcgtatc agccgtcaac aatgcccggg ctgccgacgg ctcaggaaat catcgaggca
     2161 agtttctcgt ttaccgagaa actgctagtc caacaacaga gcttcatgca gaagctggtt
     2221 gcctcgcccg agcctcggac cgaggtgccg gcggcgcccg cggtggcgat gggtaaggcc
     2281 gtgagcccga agagcaagaa cgctaactga cgctgtcgga acggattgtg gccgccctgc
     2341 ggccaccttc cgcttgctgc tcgtgtgcta attcgcggcc gtcgcctcgt gcgcgccgaa
     2401 ctgctcgagc agcgccacga tctccgcggc gttgcgctgc ttcgcgaagt acagggcgtt
     2461 gttcgtcgag tccttgtgca cggcgtgcac gtcggcgcct ttctcgagca gcgtgcggat
     2521 caatgcgagc cgcttcggca cgtcggtcaa cggcgcgttg ccgcgcgcgc gcgagtaccc
     2581 tgcgagcagc atcagcggcg tagtgccgtc ctccgtcacg accgtcgcgc tcgctccgtg
     2641 cgcgagcagc gtctctacca cggcgaagtc gccggctgcc gcggcgcggt cgagcggcgt
     2701 cgcaccgggc ggcacgagaa tctcgccggc gaccgtgcgc tcggggaggc gcggcttggc
     2761 atcgccgtcg atctcgccct tcgtgtaagg caggtccgga ttggcgccat gctcgagcaa
     2821 cgcgttgacg agatcgagcg aagtgacgtc gccgtccttc tcgggcggat tcggccgctg
     2881 cgcgtagaag cccagattgc gcgtgtcgac gacgacgtaa agcgagccgt cgtcgacgcc
     2941 gacgccgcgc tcgacgagcc gcttcgccag cgtgtagtgc ccgttgacga tcgcgagctg
     3001 taacggcgtg agctggtact gtggctcgct cttgctcgcg tcggcgcccg cgtcgagcag
     3061 cgtctccacg gcctgagcgg acccctgccg cgccgcgaat tgcagcgccg tgagcccgcc
     3121 caagtcgcgc gtcttcggca cgttaccttc cgagtagctg atcttgaccc agtcgtgctt
     3181 cgtcgaggcg cgatccacct cggcgccagc ctcgatgagc gccgccacga cgtcggcgtg
     3241 attctcgatc gccgcccaca tcagcgccgt ttgcccgtgc cagttttccg tggcgtcggg
     3301 caagccgccg tgcgcgagca gcgcacgcac gctcgcgaga tcgccggcgc gcgcggccgt
     3361 catgagcacg gtctcgcctt cgccgaaggt cgcgttggca tcgccgccgg cgtcgagcat
     3421 gagctcgagc atctcggcgt tgcgcgcggt cgctgcttcg tgcagcggcg tgagtccgaa
     3481 gcgattggcg agattcgggt cggcgccctc gctgagcagc cgtttcactg cgccgacgtc
     3541 gttattgtgc gcggcccagt gcagcgccga cgcggacgtg tctgccgacg tatgttgtgc
     3601 gaacgcagcg ggcgccgtca cgagcgccgc gacgacagcc agtgcgagac gaagctggtt
     3661 cacggcgccc ccgattgcga aattacgcga gcgacacgcg gccgttgctc gcgcacatgg
     3721 ccttgccgta gctctcggct tcgatgccgt agagctcgag cacgctgacc atgaggttcg
     3781 cgagcggcac catcttgtcg aacttcggga tcgccggaat tgcacgctcg gccgcgagct
     3841 ggccggcttg aatgtggcgg ccgcccttga gccggccgcc cgcattgccc gccagcagga
     3901 tcggcagatt caagtggttg tggacgttgc cgtcgctcat cccgctgccg tagacgaaca
     3961 gcgagttctc gagcaggttg ctgtcgccgt ccggcgtgtc cttcaagcgt tgcaggaatt
     4021 tggcgaacag gctcacgttg tacgtgtcga ccttcgcctt cttctccatc tgcttcgcgt
     4081 cgtaattgtt gtgcgagatc gggtggtggc cgtcggagat gccgatttgc ggataggaca
     4141 ggcccgacag ctcgcggccc atcatgaacg acgtgacgcg cgtgaggtcg ccttggaatg
     4201 cgagtgcctg gagctcgaac atgaggttca cgtgctcctc gaacgactcc gggacgccgc
     4261 tcggcgtcgg cggcgcctcg agccggcgtt ccgagcgctg gtcctcggct tgcacgatgc
     4321 ggcgctcgac ctcgcgcacg ttcgagaggt attcgtcgag ccgtgcgcgg tcgcgtgcgc
     4381 cgagcttgcc ggccaacccg tgcacgtcct cgacgatggc gtccaagatg ctcgtcgttt
     4441 gctcgagccg cgcgcggcgc gcttcggcgc tcgtgccgtc gccgccgaac atgcgctcga
     4501 acacgacgcg cgggttcagc tccatcggca gcggctgcgt cggcgtgcgc cacgagatcg
     4561 tgtccatgta cgtgcagagg aagtccgccg cacaggtgcc gaaaatgttc gagctgtcct
     4621 cgatcgcgag ctccatcgac gggaacgtcg tgccctggcc gatcttctgc gcgatgactt
     4681 ggtcgatcga cgtatcgagc tcgacgagat tgccgcgcgg cgcgtgcgcg caggcggaga
     4741 aaaatcccgg gcaagccgag tgcgcgttgg cgccttcgga gtagaggccc gtgacgacgt
     4801 tgaaatgctc gcgcaagccg ccgagcggcc gcaggatgat cggcagctcg aaatccgcgc
     4861 cggtcttctt cggcatccag acgtccatga tggcgccgtg cgggatgtag atcgtgccga
     4921 agcgcagcgt gcgtgccggc gcttgcgcga aagccggagt catcgcatcg agcagcggga
     4981 gggccagcga cgcgccgacg cctctgagga tcgttcgacg ggacagctgt ttcttgctga
     5041 tgaacataag tcgctccgct ctatcgagaa gaatctaacg caaccttggt atccgcgtct
     5101 gtgccggcaa tccgcatttg gaacggctcg ctcgtcacga ccccgagcac gaggctcgaa
     5161 aaacggtagt cggcgtcggc ggcctggcgc acgatcgcac gcgccgtcgg catgtcggcc
     5221 gccgtcaacg cgcgtcccag cgcatacgtc atgagctttt cggtccccgt gcgaacgaac
     5281 cgttcgggtt ggctcaggat ggcggcgcgc aggtcctcgg gcccattgat cggtccgaag
     5341 tcgcgcaaca cgtggatcgg gctgccgtaa tcgacgacgg gctcgccgtt gtcctccgag
     5401 cgccagcggc cgatgccgtc gtagcgctcg agcacgaggc cgatcgggtc catgagcttg
     5461 tgacagccgg cgcacacggg gtcggcgcgg tggcgctcca tgcgcgcgcg catcgacagc
     5521 ttctccgtcg tgttctcggg cagcgtcggc acgtcgggcg gcggctcggg cggcggcgtg
     5581 cccaagatgt tcgtcagtac gtattggcct cggctgacgg gagaggtgcg gttcgggtac
     5641 gacgacagcg tcaggatgct cgcctggccg agcaggccga aacggcgcgt gtcggtcagc
     5701 gtcacgcggc ggaactggtc gccgtagatg ctgggaatgc cgtaatggcg cgcgagccgc
     5761 tcatttaaat acgtgtagtc ggccgacaag agctccgtca gcggccggtc ctcgcgcacg
     5821 atcgtttcga acagcagctc ggtctcgcgg atcgcgctct ggcgcaagtt gtcgtcgaag
     5881 tccggaaaca cgtacatgtc cggcgacgat gcccgcaggt tgcgcaggta cagccactgg
     5941 cttgcgaagc ttttcgcgag cgtcgtcgcg cgctcgtcgg cgagcatgcg ttcgacttgt
     6001 ttgcgcagct cggcgggctt gctcaagttg ccggcgacgg ccgcgtcgac gagctcgtcg
     6061 tcgggcaagc tgctccacaa gaagaacgac aggcgcgtcg cgagctcgag gtcgctgatc
     6121 ttatataagg tccccggcgc ggcgtccgcc gggtccggct cgacgcggaa caggaattgc
     6181 ggcgcgacga gaatataggc gaggcccgtc tgcacgccgg cttcgaagtc gcggcctttg
     6241 gcgcgctcgg cttgatagag cccgacgagt cgctcgcgct ctgcatccgt gatcggccgg
     6301 cgatatgcgc ggcgcgcgag cgtgctcagg atgtcggtcg cgcacggcgc ttcttcggcg
     6361 gccgcggcgg gatagcagga gaagatccgc gcgcggctcg gcgaatggcc gggcccggcg
     6421 atcgagagcg gtccttcgat gacgatctgg tcgagctcgg gaatgccgac gtccatgcgc
     6481 gcgtcgtgct tctcgcgcgc gaacggctgc agcacctcct ggcgcgtcgc ggagctcttt
     6541 ttcgcgaacg cgacgccgac gtcgtgctgg ccggccgcaa cggtcacgcg cgtctcgaaa
     6601 cgccggtcga tctcgtcggc cgtcttgccg gggaacatct gcgccggcac ttcgtcgtcg
     6661 ggaccgccga agcgcgtcag cgacagccgc gcgccgtcga ggctgacctc gaggtcgtgt
     6721 ggcgtctcgg tgccgcgcac gacgtcgacc gtgttgcgcc acagccgcgg ccggatgacg
     6781 tactcgccgt cgacggggaa gtggtgactc acgagcatgc cgccccgcgt accgagcggc
     6841 aggccttcga cgtagtccgt ctgcgagcgg tccggcggca cgcggtaagt cgacacggcc
     6901 ggcgcaatgt ccgtgtcgcc gatcgcggcg ctcgtgacct tccacgccgc cgacagatag
     6961 cgatccatca acgacggatc cgtgccgagc acgtcggcga tgttgtcgaa gccgtacgct
     7021 tcgctgtcgt tcggcaggag cgcggtcgcg ttgacttcga gcgccagcag gtcgcggatc
     7081 gcattgccgt attcggtgcg gttcaaccga tgcagcgact tcaagcccgg gttcgggttc
     7141 tcggccgccg cggcatcgag ccgggtttcg aggaaggcga cgagctcgtt cgcggtggcc
     7201 gcctcgggca gcggctcgcc ggccggcggc atcatgcggc cgcgcagctt tttgacgacg
     7261 gtttcccaga cctcggcgtg agccccgacg tcggtcagtg cgagcccttc gagcgagagc
     7321 tcgcccgtcc gctcggcgtc gttatggcag tcggtgcagt accgctcgat gacgtccttc
     7381 gcggcagccg cacgctgctc atcgctcggc tggcaagcgg cagaggcgag ggcgagcagg
     7441 gcgatgacga cgggtacacg cataacccca aaaacctacg ggaaaaaagc tcccggcggt
     7501 agggtctagc gtcaagaact catcagttgc caggctgtgt gaactcgaaa gtcagctctg
     7561 agcagggctg cttctcgaac ggtgtttccg aaagcagaac gacatcctgc cccttgtacg
     7621 gcgctgtgag gtagacgggg tcctccgcgg tgtactcgcg cttcagcgca agcgtctgcg
     7681 gatccagcgt gaagcgctcg acgacgtgca gctgcgggct gttgcgcgtt ggcggcacga
     7741 gcacaccttc ggcaaacccg accgtgtcga cgacgagcgt gtccccgtcc cagcgaccga
     7801 tcgaatgacc agagttgctc ggcgccagat tcgcgggatg cgcgtccatg ccgacatgga
     7861 tgcggcgtgt ggcgctgaac aggccgtaat cgatgtcgat gaccttctcg ccgcccatcg
     7921 tcgtttgcgt gatgcgattg accggccagt cgaaggtcca gtcgaagatg atgctcgtcg
     7981 gtttgcacga caagcgcggg ttatcctccg tcgaccacat gcgaaacgcc gtcgcggctt
     8041 cctggccttt ggccgtgtac tgcgggcgcg acggttgagg attgagcgcg cggatctgct
     8101 cgagcgtgat cttgccggcc gcgaagtcct cgcgcaggct gcgcggcacc agcgagccgt
     8161 tgccgccgct cggcgggatc gtcaggacgg cttgctcgac ggcccagtcg cccgagatgt
     8221 tcggctcgcc ggacgacagc cgccccggcc gcttcgacgt gtcgacgggc gcgtgcgtga
     8281 actggtcgtt gcggtcgatc gtcgtcgtgc cgccgatcgc gacgctctcg agatagcacg
     8341 agtgcggatc gtcgcggtgc ggatgcgcgt ggatgtcgac gtgccggccg ggcacgaact
     8401 cgtcggtctt ccaccccgag cggcggatca gcgtcgccgc gcgcatctcg cagcgcatct
     8461 cgagcggttt gccgtcggcg ccgaaggtct cgaagtagag atacgaatgc gggttgacga
     8521 gctcgagctt cgtcagcgtg cccgagtagt ccttgaattc ctgcatctgg aacaacccga
     8581 acccatgatg cgcgagcgcg ggagctgcgg cggcgagcgc cagtgcgggc gcggcggcgt
     8641 agcgatggcg acttggcatg gtgagtcctc aattgcgcgg ctcgcaggcg ctcgatcgtt
     8701 cacggctgtc gttatcttac cgcgcgtgcg gagtgtcgcg tgcgcacctc gccgttggtg
     8761 caggctgccg cacctcgcgg ctcgaatctt cagtctctcg gcgcgctgca ccggcgccgt
     8821 ggagctcgtc acggcctgac gcgcacgtac ttctgcaggc gccgcggccg tggttcgccg
     8881 ccatagatgt tgccgtcgcg gtcgacgacg gcgaactcgg cgccggtgcc ggcgcgcacg
     8941 cgcgggtcgc cccacggata cagaatgaac tcgcgcacgg ccccgctcgc ggcgtcgccg
     9001 atgcgaatgc ccatgtccca gcccgggttc tcgatgtcgt cggattcgga gtccgcgacg
     9061 atgatgcggc ccaagtggtc gaagaagatg ccgctcggcc ggccgaactg cgtccagctc
     9121 gtgaggtacg tgccttcctg gtcgaagagc tgaatgcgat tgttgccgcg atcgccgacg
     9181 aacacgcggc cctgcgcgtc gatggcgatc gcatgcatcg tccggaactc accgggaccg
     9241 tagccggtgt ggccccactc cttcagatag cgcccgtcgg ctgcgaactt cacgacgcgg
     9301 ttgttcgtgg catcgccgtg accgtcggcg acgaagatgt cgccgttcgg cgcgacgacg
     9361 atgtcgctcg gtgcgttgaa gtgcgtgcga tcgttgccgg cgacgcccgc cgtgccgagc
     9421 ctgagcagca cctcgccgcg cgggctgaac ttgatgactt ggtggccgcg accacggccg
     9481 ttcggcccat tcgtgcgctg ttcgtcggcc gcgtcggtga cccagacgtt gccttgcggg
     9541 tcgacctcga ggccgtgcgg ccagatgaag agcccgccgc cgaagctttc gacggcgttg
     9601 ccgtcgggcg cgaacttgat gacgacgtcg agatccgact cgaggcattc gtaaccaaac
     9661 ttgtcgccgc cggcatcgca gcgcaggacg gcccaaacat ggcgcccgtc cacgtcgatg
     9721 tcgacgtcgc cgacggcgcc gatctcacga ccgctcggga gcttcgccca gttgtcgacg
     9781 aggcgatacg gattcggcag cgcttgcgcc agtgccaccg cgggcgagcc gagcaccaga
     9841 gcgagcgcga tccaagtgca gctgttgttg ttcatcgtga gcctcctttt ggctggcgcg
     9901 gcgcccgcca gccggcggtg cgcgcttcgc cctcggtgca aaaccatcgc tcgcccttgc
     9961 tttcgtctat cgccgtatct gcgtatgacg aagagccggg cgtgtgataa atgcgctccc
    10021 cgtcgccgtt aatgttgcct ttgatgtagc agccgtcgcg ctgcgattgc ggaggcgggg
    10081 cagcggcgcg ttctcgcggc gtgtctcgtt gctcgttgcg gtaggtctcg ggaggggtga
    10141 actcgccggc ccaaatgccg cgacgcgccc tgcgcgcttc gttctcctcg tcgacgtagt
    10201 cgttgctgta acgacgatac gcggtcgcca tgcccgcgcg gaccatcgct tcgcccaagt
    10261 ccgttgtgcc gactcggcag acggcgacga tgcggccgta agtgtccttg tcgcgctgct
    10321 cgcacgcgac gtcgcgcgag ccgacgaggc gacgcagctc ggcggccgcc gagtcgccgc
    10381 aactccagtc gcggccgtcg cgagtgcacg attgccggcc ttcaggcgcg tcgacgccga
    10441 acaagcgcac cccgacgtta ccgagctcga acgaatcacc gtcgatgagc ttcggccgcc
    10501 ccgcgaggcg ctcttgcggc gtgcatgcgc gttcgccgcc tacgcccgcg agcgtcacga
    10561 tcgagaccaa gaccaacgcc atcagcctgc gcatggtggc gtgacgatac gccgctgccc
    10621 ggcggctcga aaggccggtg taccatcgca agcatcgaca aatggaggtg gcgtcgatgg
    10681 cgaagcgagt gacactgcta acttgtgcga tcgcggcggc gttcgcggcc tgcggcgact
    10741 tcggtgggtc ctttgcgcaa agcccgttca cgaccacccc cgtcgccgag ttcaacgagc
    10801 cgtgggcgat ggcgtttctg cccgacggcc gcttgctcgt cacggagaag cgtggggcgc
    10861 tcaagctcca ctcgctcgaa accaaacgta ccggtgatgt cgccggcgtg cctgccgtcg
    10921 cgtacgcggg gcagggcggt ctcggcgata tcgtcttgca tccgaatttc actacgaacg
    10981 gtctcgtcta cttgagctac gcagaagcgg gcgagcgtgc tttggccggc gcggcggttg
    11041 cgcgcgcccg gctcgtgctc gacaagtcga ccggcaggct cgaaaacctt gcggtcatct
    11101 ggcggcagac gccgaaggtc gacggccgcg gccactacgg gcatcgcatc gcgttcgggc
    11161 ccgacgggtt cctctatatc agctccgggg agcggcagaa gttcacccct gcgcaggacc
    11221 tgcagcaaaa cctcggcaag atcgtgcgct tgagcgacga cggcagcgtg ccggccgaca
    11281 acccgttcgg cgaacgcggc ggcgtcaccg cgcaaatctg gtcgctcggc catcgcaacc
    11341 cgctcggtct cgcgttcgac gcgcaggggc agttgtggaa cgtcgagatg gggccgatgg
    11401 gcggcgacga gctgaatcgc gtgcaacgcg gcgcgaacta cggttacccg ctcgtcagca
    11461 acggctctca ctatggcggc gcggacattc cggatcacga cacgcggccc gatctcgcag
    11521 cgcccgctgt tttctggaac cccgtgatct cgccgtcgag tctccttttt tatagcggca
    11581 gcgagtttcc ggattggcag ggcgacgcct tgatcggcgg gctgtcctcg acctcgatcg
    11641 tccggatcga gttcggcgca ggcggcacgg cgcgcgaggc ggctcggtac cgcatgccgc
    11701 agcgaatgcg cgcgctcgag caggggcccg atgggtccat ttgggcgctc gaggatggcc
    11761 gcggcggccg gctcctcaag cttatggccg cggcgcagag ccgctaacgc tttggcgcga
    11821 ttgtgggttt agaatcggtc gcaccagatt gggagatcgt catgcgttcg tcgcacttcg
    11881 tcacgttcgt cgcgggcctc gcagcggccg gtctggcagg gctagctgcg gcacaccaca
    11941 gccaagtcgg gattttcgac tcgaaagaga acatcgaggt tacaggcgtc atcaagtcga
    12001 tctcgtggcg caacccgcac gggcagatcc tgctgacgca gacgaatccc gacggtacga
    12061 cgacggagtg ggatgccgag acggcgtcga ttgccgtgat gcgcaatcgc ggcaccgagc
    12121 cgagcgccgt cgtcgtcggc gatcgcgtga cgatcgcggg agcgccgagt gtgcgcgggc
    12181 gaaaggagat cctcgcgcgc agcatgctgc tgtcgagcgg ttacgaattc acgttcggcg
    12241 gcgcgcaggc gtactttccc gaaggcaaaa gcggccgcat cgtcggccgc gcgacgatcg
    12301 aagccgacgt caaggaggcg acggcgaagg ccgacggcat cttccgcgtt tggtcgacga
    12361 tcatgagcga ccccgcggcg tttccaatgt tcaaaggcgg atatccgctg acggccgcgg
    12421 gccgcgcggg cctcgcgcaa tggaacccgc gcgacaactc gctgctgaag tgcggtacca
    12481 agggcacgcc gctgatcatg atcacgccgc tgccggtgga gttcgtgcgt gacggcgaca
    12541 agatcacgat gcggatcgag gaatacgaca cggtgcgcac gatccacatg aacgcgaacg
    12601 cagcagcgcc ggccgagcac acgctgctcg gcttctcgcg cggccggttc gaaggcaaca
    12661 cgctcatcgt cgagaccgat cacatcaagc ccggctattt cgatcacgaa ggcacgccga
    12721 tgagcgatca gatcaaagtc gtcgagcgct tcacgccgaa tgccgactac actcggctgg
    12781 actacaagct caccacgacc gatccggtca acttcgagcg gccgttcgac ctgacgcgct
    12841 atttcatctg gaagcccgag aacaccgtgc acccgtacga gtgcctcgat cggttctgag
    12901 cggcgagggc gctcgtctca gacgagcacg atgagccagt tcaatgcggc caacacgctt
    12961 cccatcagcg tggtggccgc accgaatgcg accccccagc gcgcgagctc gggccggtgc
    13021 tcgatgcagt agagcagcgg taagccgggg accgtcgccg cggagtgcag gcagtaggcg
    13081 atgcgcggcc agcgcgtgct gaaagtcaga agctccgagt gcgcgaggac tgcggctgcg
    13141 atcacgcgct cgtcgcccag cgtcgcgcgc gccgtgcgcg tggcttcctc ggcgctgagc
    13201 ccggacgcgc gggccgcgct ctcgagatcg tcgtagtgct cgcgcagctc cagaatcgtg
    13261 cgctcgacgt agaccggtgt gacgccgcgc tgcagcagct cgagccgtaa cgactcgaac
    13321 tgcgcgtcac gcatgccctg ggtgtccgtg cgctccgttg agggcggagt tgacgcccga
    13381 gctgacgcgc ttccactcgt cgctcaagtg ctcgagccgg cgtttgcctt tcgtggtgac
    13441 ggaatagtag acgcgctccc ggccgttgac gctcttgcgg cgcgctttca gcgccccgcc
    13501 ttcctcgagc gaatgcagga cggggtagat gacgccctcg ccgaagccga tggcctcggc
    13561 ggtgacgagg cgcatcgcct tgacgatctc gtagccgtac atctcccggc cgctgagcat
    13621 cctgagcacg agcagctccg gcacaccggt cagaaatgga gggttcgttt gtgacatgca
    13681 atcaccatag acccaactcg gtgtactcgc cagtccgatt tgccatttga ctccggcgtt
    13741 agttttcgag cgtgaaccga atcaacgtct cgatgcccac gcgttccacc gcgacgccgg
    13801 cgtctacgcg cgggttgtag cgccaacgcg ctatggcttt gagtgccgcc tcatcgaaga
    13861 cgcgtgatgg ctccgatcgc acgacaaccg cgtcccgaac ggtgccggcc tcggtaatcg
    13921 tgaaccgaac ttgtacccac ccttccgttc cgttccgtgc cgcgctcggg gggtaatcgg
    13981 ggttgacccg aacgcgtggc gtgacatcgc gatccgcgcc catcggcatg cctgttcttg
    14041 gcggccgagc aacctccgta agttgctgcg gaatcggccg gatcggttcc gtcggcaggc
    14101 cgcggatacg cggattgatt acgagcgtct gcgccggtgg cggctgcaac ttcgtgggcc
    14161 gtcggggcac gatcggcgtc atcacgcggt ccgcggtgaa tttaatgatg cgggcctttt
    14221 cgatcggcgg tgcatcgaat ggcacgctta cgagactcca caagcccagg aatagcccga
    14281 cgctgagcac agcgcctgcc
//
LOCUS       DATLEE010000718         4247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11064760, whole genome shotgun sequence.
ACCESSION   DATLEE010000718 DATLEE010000000
VERSION     DATLEE010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11064760"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VM692_15755"
     CDS             complement(<1..787)
                     /locus_tag="VM692_15755"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HUQ53683.1"
                     /translation="MARSALHLKDHWQEQRLFLSRIIAAAVIVVLLTGLLVTRLVQLQ
                     ILDFERFVELSQGNRFRIEPMPPTRGLIYDRNGIVIAENRPNWELVAIGEQIDDLDRT
                     LQALEDLELVDPSEHANLRDLIRSHRGFERVKLSNLTEEQAATFAVRRHLFSGVDIQE
                     ALSRFYPFGEVTAHAIGYVGSISTADLERIDRGNYAGTSHIGKTGIERAYEERLHGKV
                     GYRQQVVNAQGRVIDPLGNSRIKALADTSGIEPKIPVPGQNVVL"
     gene            complement(791..1279)
                     /gene="mreD"
                     /locus_tag="VM692_15760"
     CDS             complement(791..1279)
                     /gene="mreD"
                     /locus_tag="VM692_15760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HUQ53684.1"
                     /translation="MAARRSNWFASLLSLAATLALASVPLPDSVAPLRPDWVAVVLLY
                     WSLMAPRQFSLLTAFWMGIVLDTLTGALLGQNALALLVVVYLAEKFHLRLRVFPVSQL
                     AVTVLILLGLYEFILFWVDGMAGRSVPLIERWLPPVTGTLAWLAMYMLFDRREREAPA
                     RF"
     gene            complement(1279..2178)
                     /gene="mreC"
                     /locus_tag="VM692_15765"
     CDS             complement(1279..2178)
                     /gene="mreC"
                     /locus_tag="VM692_15765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HUQ53685.1"
                     /translation="MSATGRATPGFGRSGFSMGVRFLLLAVVCIALMLLDRRDQHLVR
                     MRQALTVVVYPVRMLVDLPFSTWITMRDTFAARDALISENQQFRRERLETEGRLQRLE
                     ALEAENARLRELLDSTARIGSRALVAEILAVDLDPYRQRFELNRGLVDGVYVGQALID
                     AQGVVGQIVRVGPLTSEAVLITDADHAVPVTVNRNSLRTIALGTGDSSRLRLPYLTNN
                     AKIEVGDLLISSGLGGVFPAGYPVGRVLSVERRPDQAFAEIVAEPVSSLDRDREVLLV
                     WNSADEVPRAAEAAAEPSVAESQ"
     gene            complement(2215..3258)
                     /locus_tag="VM692_15770"
     CDS             complement(2215..3258)
                     /locus_tag="VM692_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HUQ53686.1"
                     /translation="MLKSFLGYFSNDLSIDLGTANTLIYVRGRGIVLNEPSVVAIREE
                     PGRGGKVLEAVGIEAKNMLGRTPGHITAIRPMKDGVIADFSVTEKMLQHFIKKAHGSR
                     YFRPSPRVLVCVPYGSTQVERRAIRESAEGAGARKVYLVEEPMAAAIGAGMPVHEARG
                     SMVLDIGGGTSEVAVISLNGIVYASSVRIGGDRFDDAITNYVRRNYGILIGEATAERI
                     KHEIGSAYPGQDVREISVKGRNLSEGVPRSFTLNSNEILEALQEPLQGIVGAVKVALE
                     QTPPELGADVAERGIVLTGGGALLTDIDKLLTEETGLPVVIADEPLTCVARGGGRVLE
                     LLDEYGPSAFSVE"
     gene            3359..3700
                     /gene="gatC"
                     /locus_tag="VM692_15775"
     CDS             3359..3700
                     /gene="gatC"
                     /locus_tag="VM692_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HUQ53687.1"
                     /translation="MRSPDFIARVVSKTSTENPVLRPEDIQRLCRLARLEITADEVAD
                     VSAKLSSIVAMVDQLQAIDTAGVAPMAHPLDRPQRLRADTVTELDQHELYQRNAPLVE
                     RDLYLVPKVIE"
     gene            3750..>4247
                     /locus_tag="VM692_15780"
     CDS             3750..>4247
                     /locus_tag="VM692_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HUQ53688.1"
                     /translation="MHTRTLAQLAGDLRARRVSATELTRLYLERIERFGPALNAFITV
                     TADQALAQAAAADQRLAKGDAGLLAGLPLAHKDIFCTSGVKTSCGSRMLDNFVAPYDA
                     TVIERLRGAGAVMLGKTNMDEFAMGSSNETSFYGPVRNPWDPKKVPGGSSGGSAAVVA
                     ARLAPA"
BASE COUNT          755 a         1424 c         1376 g          692 t
ORIGIN      
        1 acagcacgac gttttggccc ggcaccggta ttttcggctc gatgccgctc gtgtcggcga
       61 gcgctttgat gcggctattg ccgagcgggt cgatgacgcg cccctgcgcg ttcacgactt
      121 gctgccgata gcccacctta ccgtgcagcc gctcctcgta cgcgcgctcg atgccggtct
      181 taccgatgtg cgacgtgccg gcgtaattgc cgcggtcgat gcgctcgaga tcggccgtgc
      241 tgatactgcc gacgtagccg atcgcgtgcg ccgtgacttc gccgaacgga tagaagcgcg
      301 acagtgcctc ttgaatgtcg acgcccgaga acagatgacg ccgcacggca aacgtcgcgg
      361 cctgctcttc cgtgaggttg ctgagcttga cgcgctcgaa cccgcggtgc gagcggatca
      421 gatcgcgcag gttcgcatgc tcggacggat cgacgagctc gaggtcctcg agcgcctgca
      481 atgtgcggtc gagatcgtcg atctgctcgc cgatcgcgac gagctcccag ttcggccgat
      541 tctcggcgat cacgatgccg ttgcggtcgt agatgagccc gcgcgtcggc ggcatcggct
      601 cgatgcggaa gcgattgccc tgcgacagct cgacgaaccg ctcgaagtcg aggatctgca
      661 gctgcacgag ccgagtcacg agcagtccgg tcagcagcac cacgatgacg gcggccgcga
      721 tgatgcgcga caggaacagg cgctgctcct gccagtgatc cttgaggtgt agcgccgatc
      781 tcgccatgcc tcaaaacctc gcgggcgctt cgcgctcgcg ccgatcgaac agcatgtaca
      841 tcgcgagcca agccagcgtg cccgtcacgg gcggcagcca gcgctcgatc agcggtaccg
      901 agcggccggc catgccgtcg acccagaaca agatgaactc gtagaggccg agcaggatca
      961 ggaccgtgac cgcgagctgc gacacgggga agacgcgcag ccgcagatgg aacttctcgg
     1021 cgagatagac gacgacgagc aacgccaacg cgttttggcc gagcaacgcg cccgtcagcg
     1081 tgtcgagcac gatgcccatc cagaatgccg tcaacaagct gaactgccgc ggcgccatca
     1141 gcgaccaata cagcagcacg acggcgaccc aatcgggacg cagcggcgca acggaatcgg
     1201 gcagcggcac cgacgcgagc gcgagcgtcg ccgcgagact cagcagcgag gcgaaccaat
     1261 tgctgcggcg cgcggccact actgcgactc cgcgacgctg ggctcagcgg cggcctctgc
     1321 ggcgcgcggc acttcgtcgg ccgagttcca aacgagcagg acctcgcggt cgcggtcgag
     1381 cgacgacacg ggctcggcga cgatctcggc gaacgcttgg tcgggccgac gctcgaccga
     1441 cagcacgcgg ccgaccgggt aaccggccgg gaacacgccg ccgagccccg atgagatcag
     1501 caagtcgccg acctcgatct tggcgttgtt cgtcaggtac gggagccgca gccggctgct
     1561 gtcgcccgtg ccgagcgcga tcgtacgcag gctgttgcga ttgacggtga cgggcacggc
     1621 gtgatcggcg tccgtgatca agacggcttc ggaggtcagc gggccgacgc gcacgatctg
     1681 cccgacgacg ccctgcgcgt cgatcaatgc ttggccgacg tagacgccgt cgacgagacc
     1741 gcggttcaac tcgaagcgct gccggtaggg gtcgaggtcg acggccaaga tctcggcgac
     1801 gagcgctcgg ctgccgatgc gagccgtcga gtcgagcagc tcgcgcaagc gcgcgttctc
     1861 ggcttcgagc gcctcgagcc gttgtaggcg gccctcggtc tcgagacgct cgcggcggaa
     1921 ctgctggttc tcgctgatca gcgcgtcgcg agccgcgaac gtgtcgcgca tcgtgatcca
     1981 cgtgctgaaa ggcagatcga cgagcatgcg cacgggatag acgacgaccg tcagggcctg
     2041 gcgcatgcgc accaagtgct ggtcgcgccg gtcgagcaac ataagggcga tgcaaacgac
     2101 ggcgagcagc aagaagcgca cgcccatgga gaacccgctt cggccgaagc ccggcgtggc
     2161 tctaccggtc gcactcatcc gtgatcgctc ccctgcgcgt tgtgcgagtg gtggttactc
     2221 caccgagaaa gccgacggtc cgtactcgtc gagcaactcg agcacgcgcc cgccaccccg
     2281 ggcgacgcat gtcagcggct cgtcggcgat gacgaccggc agcccggtct cctctgtcag
     2341 cagcttgtcg atgtcggtca acaatgcgcc gccgcccgtc agaacgatcc ctcgttctgc
     2401 gacgtcggcg ccgagctcag gaggcgtctg ctcgagcgct actttcaccg cgcctacgat
     2461 tccctggagt ggctcctgca gtgcctcgag aatctcgttg ctgttcagcg tgaagctgcg
     2521 cggcacacct tccgacaagt tgcggccctt gaccgagatc tcacgcacgt cttggcccgg
     2581 atacgccgag ccgatctcgt gcttgatgcg ctcggccgtc gcttcgccga tcaagatgcc
     2641 gtagttgcgg cgcacgtagt tcgtgatcgc gtcgtcgaac cgatcgccgc cgatgcgcac
     2701 cgacgacgcg tagacgatgc cgttcaacga gatgaccgcg acctccgacg tgccgccgcc
     2761 gatgtcgagc accatcgagc cgcgcgcttc gtgcacgggc atgccggcgc cgatcgcagc
     2821 cgccatcggt tcttcgacga gataaacctt gcgagcgccg gcgccttcgg ccgactcgcg
     2881 aatcgcgcgg cgctcgactt gcgtcgagcc gtacggaacg cacacgagca cgcgcgggct
     2941 cggacggaag tagcgggagc cgtgcgcctt cttgatgaag tgctgcagca ttttctcggt
     3001 gacgctgaag tcggcgatga cgccgtcctt catcgggcgg atcgccgtga tgtggcccgg
     3061 cgtacggccc agcatgttct tcgcctcgat gccgacggcc tcgagaacct tacccccgcg
     3121 gccgggctcc tcccggatcg ccacgaccga cggctcgttc aacacgatac ccctgccccg
     3181 gacgtaaatc agggtgttgg ccgtgccgag atcgatggag aggtcgttgg agaagtatcc
     3241 gagaaaactc tttaacattg aggcacaagg ccgctggggg aatcgtggcg cgactgtatc
     3301 aacgggtagg ccaatccaca agcgacgtgt attatctaat gagaagtcgg tcacttcatt
     3361 gcgcagcccc gatttcatag cccgcgtcgt cagcaaaacc agcaccgaga accccgtgct
     3421 tcgccccgaa gacatccagc gactgtgtcg gctcgctcgc cttgaaatta ccgccgacga
     3481 ggtcgccgac gtctcggcca agctgtcgag catcgtggcc atggtcgacc agctgcaggc
     3541 tatcgacacg gccggcgtcg ctccgatggc gcatccgctc gatcggccgc agcggttgcg
     3601 cgccgacacc gtgaccgagc tcgaccagca cgagctctat cagcgcaacg cgccgctcgt
     3661 ggaacgtgat ctgtatctcg ttcctaaggt catcgagtaa ggctaaacgc gctcacgcga
     3721 accgccgacg cggttggcaa tcgattgcta tgcacacacg tactctcgca cagttagccg
     3781 gtgacctccg ggcgcggcgc gtctccgcga cggagctcac gcgtctctac ctcgaacgca
     3841 tcgagcgctt cggtcctgcg ttgaacgcat tcatcacggt gaccgccgac caagcgctcg
     3901 cgcaagccgc ggccgccgac caacgcctcg ccaaaggcga cgcgggtttg ttggccggct
     3961 tgccgctcgc acacaaggac atcttttgca cgtcgggcgt caagacatcg tgcggctcgc
     4021 gcatgctcga caacttcgtc gcgccgtacg acgcaacggt catcgagcgc ctgcgtggcg
     4081 ccggcgccgt catgctcggc aagacgaaca tggacgagtt cgcgatgggc tcgtcgaacg
     4141 agacgagctt ctacggaccc gtgcggaacc cgtgggaccc gaagaaggtg ccgggcggat
     4201 cgtccggtgg ttccgccgcg gtcgtcgccg cgcgtctcgc gccggcc
//
LOCUS       DATLEE010000719         3162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11065407, whole genome shotgun sequence.
ACCESSION   DATLEE010000719 DATLEE010000000
VERSION     DATLEE010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11065407"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VM692_15785"
     CDS             <1..485
                     /locus_tag="VM692_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUQ53689.1"
                     /translation="TVWGIPAYEAIAAAARDWPADLLVVGAHEPQSQHARLTDTDWQL
                     MRRASCPLLLVKSAAFDGYDQIVAAVEGADGGDEVVLAAGRCFARAFGSTLAVVEVGA
                     LGEAVGRRACLVVVRAPVAAVPGELEAAAETVVNSAPCDVLLVPPRRGSKKLASSVHR
                     "
     gene            complement(476..1861)
                     /locus_tag="VM692_15790"
     CDS             complement(476..1861)
                     /locus_tag="VM692_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUQ53690.1"
                     /translation="MATFVAAIGLVAAAFAALHALLHKRRPQSAFGWIAVSVAWPFVG
                     ALLYYLFGINRVEARARKLLTRHPAIDCPTEYLGTPPPQLLPLAKLGSAVSGWPLTAG
                     NKIEPLHEAAVIFDEMLAAIDSASAYVYFSTYIFDGGPVGRRFATALAAAAERGVDVR
                     VLLDGFGEWYSWRRASDLFRGTKVRFARFLPPRLWPPTINVNLRNHRKILVVDGVAAF
                     TGGINVRDRYLDGVAAERIVDSHFRLSGPVLVQIETVFLRDWQFATGDASSPLRRVPE
                     PSGGALCRVVADGPDVGLDRLTALLTGAIGSARQRIAIMTPYFVPPRELVAPLQAAAL
                     AGVDVAVLLPARNNLPYVHRATRHMLWELLERGVHVYYQPPPFVHSKLFYVDDYYAQI
                     GSANFDARSLRLNFEMNVEVYDRDTVSGLARHFEAIRARSVQVTLADVDGRSLVTRAI
                     DGVAWLFSPYL"
     gene            1991..2908
                     /locus_tag="VM692_15795"
     CDS             1991..2908
                     /locus_tag="VM692_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005768408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HUQ53691.1"
                     /translation="MEYKDYYQTMGVARDASADDIKRAYRRLARKYHPDVSKEKDAEA
                     RFKEIGEAYEVLRDPEKRAAYDALGARKPGEDFRPPPDWEFGHGVQGDAGAHSDFFEQ
                     MFGGLGARRGSFRSRGFDTAGQVEVTLEEAFSGTERRLSLERVAVDERGRAQPTTQQL
                     NVRIPAGVTDGQQIRVPAQGQPGVGGGPAGDLFLQVRLQPHRWFRAEGRDIFLDLPVT
                     PWETALGETVRVPPLGGRVDLKLPKGAQTDQQLRLKGRGLPGKPPGDQFVILKIKAPS
                     ATSPDGEELYRQLKAALPTNPRQAMEGGS"
     gene            2905..>3162
                     /locus_tag="VM692_15800"
     CDS             2905..>3162
                     /locus_tag="VM692_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HUQ53692.1"
                     /translation="MTTPISATPLDDSVELTLAELCSACSVEEELVIEIVAEGIVEPI
                     GPSRAQWRFSGLAVTRVQRVLSLQHDFGVNLPAAALALELLE"
BASE COUNT          531 a         1023 c         1140 g          468 t
ORIGIN      
        1 gcacggtttg gggtatcccg gcctacgagg cgatcgcggc cgcggcgcgt gactggccgg
       61 ccgacttact ggtcgtcggc gcacacgagc cgcaatcgca gcacgctcgc ctcacggaca
      121 cggactggca gctcatgcgg cgagcgtcgt gcccgctgct gctcgtcaag agcgcggcgt
      181 tcgacggcta cgaccagatc gttgccgccg tcgaaggggc ggacgggggc gacgaggtcg
      241 tgctcgctgc cggccgctgt ttcgcgcgcg ctttcggctc gacgctggcc gtcgtcgaag
      301 tcggcgcgct gggcgaggca gtcgggcggc gcgcttgcct cgtcgtcgtg cgtgcgccgg
      361 tcgccgccgt acccggagag ctcgaagccg ctgcggagac cgtcgtgaac tcggcgccgt
      421 gtgacgtatt gctcgtgccg ccgcggcgcg ggagcaagaa gttagcgtcg agcgttcaca
      481 ggtagggtga gaacaaccag gcgacgccgt cgatcgccct cgtgacgagg gagcggccgt
      541 cgacgtcggc caacgtcact tggacggagc gagcgcgaat cgcctcgaag tgccgagcga
      601 gcccggacac cgtgtcgcgg tcgtacacct cgacgttcat ctcgaagttc aagcgcaggc
      661 tgcgcgcgtc gaagttcgcc gaaccgatct gcgcgtaata gtcgtcgacg tagaacagct
      721 tgctgtgcac gaacggcggc ggctggtagt agacgtgcac gccgcgctcg agcagctccc
      781 acagcatgtg ccgcgtggcg cgatggacat agggcaagtt gttgcgtgcg ggtagcagca
      841 cggcaacgtc gacgccggcc agcgcggccg cttgcagcgg cgcaaccagc tcgcgcggcg
      901 gcacgaaata cggcgtcatg atggcgatgc gctggcgcgc gctgccgatt gcacccgtca
      961 gcagcgccgt aagccggtcg agcccgacgt cggggccgtc ggcgacgacg cggcacagcg
     1021 cgccgcccga cggctcgggc acgcgccgca gcgggctgct cgcgtcgccg gtcgcgaatt
     1081 gccagtcgcg taggaacacc gtctcgattt gcacgagcac ggggccgctc aggcgaaagt
     1141 gcgagtcgac gatgcgctcg gcggcgacgc cgtcgagata tcggtcgcga acgttgatac
     1201 cgcccgtgaa ggcggcgacg ccgtcgacga cgaggatctt gcggtgattg cgcaagttca
     1261 cgttgatcgt gggcggccac agccgcgggg gcaagaagcg tgcgaagcgg accttcgtgc
     1321 cgcggaacaa gtcgctcgcg cgtcgccacg agtaccactc gccgaaaccg tcgagcaata
     1381 cgcgcacgtc gacgccgcgc tcggcggcgg cggcgagcgc ggtcgcgaac cgccggccga
     1441 ccggcccgcc gtcgaagatg taggtgctga aatagacgta ggcgctcgcg ctgtcgatgg
     1501 cggcaagcat ctcgtcgaag atgaccgcgg cctcgtgcaa cggctcgatc ttgttgccgg
     1561 ccgtgagcgg ccagccgctg acggcggagc cgagcttcgc gagcggcagg agctgcggcg
     1621 gcggcgtgcc gaggtattcg gttgggcaat cgatggccgg atgacgcgtc aacagcttgc
     1681 gggcgcgcgc ttcgacgcgg ttgatgccga acaaataata gagaagcgcg ccgacgaacg
     1741 gccaggcgac ggagacggcg atccagccga acgccgattg cgggcggcgc ttgtgcagca
     1801 aggcgtgcag agcagcgaag gcggccgcaa cgaggccgat ggcggcaacg aaagtcgcca
     1861 atagctgacc tgcctgcatg gcgcgatgtg taacataggc ctagaggtac ggccagcggc
     1921 ggtggttgaa acttcgggct tgcgccgcca ctacgtggac ctctgcgaaa ccggatacgc
     1981 ctaggccgac gtggaataca aggattacta ccagaccatg ggtgtcgccc gcgatgcgtc
     2041 cgcggacgac atcaagcgcg cgtatcgccg cctcgcgcgc aaataccatc ccgacgtcag
     2101 caaagagaaa gatgccgagg cccgcttcaa ggagatcggt gaagcgtacg aggtgctgcg
     2161 cgatcccgag aagcgcgcgg cctacgatgc gctcggcgca cgcaagcccg gcgaggactt
     2221 tcgcccgccc cccgattggg agttcggtca cggcgttcaa ggcgatgccg gtgcgcacag
     2281 cgacttcttc gagcaaatgt tcggcggctt gggcgcgcgc cgcgggtcgt ttcgcagccg
     2341 cggcttcgac acggcggggc aggtcgaagt cacgctcgag gaagcgttca gcggcaccga
     2401 gcgccgcctg tccttggaac gcgtcgccgt cgacgagcgc ggccgggcgc aaccgacgac
     2461 gcagcagctc aacgtgcgga ttccggccgg cgtcaccgac ggccagcaga tcagggtgcc
     2521 ggcgcaaggt cagcccggcg tcggcggcgg cccggccggc gacctgttcc tgcaagtccg
     2581 ccttcagccg catcgctggt tccgcgctga aggccgcgac atctttctcg atctgcccgt
     2641 gactccgtgg gagacggcgc tcggtgagac cgtgcgcgtt ccgccgctcg gcggtcgcgt
     2701 cgatctcaag ctgccgaagg gggcgcaaac ggatcagcag ctgcggctca aggggcgcgg
     2761 cttgcccggc aagccgcccg gcgatcaatt cgtcattttg aagatcaagg cgccctcggc
     2821 tacgagcccg gacggcgagg agctctatcg gcagctcaaa gccgctctac ctacgaatcc
     2881 gcgccaggcg atggagggcg ggtcatgacg acaccgatca gcgcgacgcc gctcgacgac
     2941 tcggtcgagc tgacgctcgc cgagctgtgc tcggcgtgct ctgtcgaaga ggagctcgtc
     3001 atcgagatcg tggccgaggg tatcgtcgag ccgatcggcc cgagtcgcgc gcagtggcgc
     3061 ttctcgggtc tcgcagtgac gcgtgtgcag cgcgtgctga gcttgcagca cgacttcggc
     3121 gtgaacttac cggccgccgc gctcgcgctc gagttgctcg ag
//
LOCUS       DATLEE010000720         4449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8836765, whole genome shotgun sequence.
ACCESSION   DATLEE010000720 DATLEE010000000
VERSION     DATLEE010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8836765"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VM692_15805"
     CDS             complement(<1..575)
                     /locus_tag="VM692_15805"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ53693.1"
                     /translation="MCNNDRTAARATLAARALLVATIVLGAGAAGNAAVLEAVKDNDG
                     AGLRGLLADGADPNEADSDGTAALHWAVHQGQSETVAALLSAGAAVDAPNRYGVRPLY
                     LAAENGDASMVRALLEAGADPNGARVEGETALMIAARTGDVATLEALIAAGADVNAVE
                     ERKGQTALMWAAADNNGAAVKALLAAGAARDA"
     gene            complement(568..1914)
                     /locus_tag="VM692_15810"
     CDS             complement(568..1914)
                     /locus_tag="VM692_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HUQ53694.1"
                     /translation="MIVTKRALSRRTFLRGSGAAIGLPLLDAMVPAFAADAPKVTRLG
                     FIYLPNGVSMNFLGVNNWIPKGEGRNFELSQILTPLAGHREHLTVVTGLAQRPAEASE
                     ESATGDHTKATASWLSGVRPKRTEGADLENGVSTDQLAADVIGKDSVLRSLELSIDLS
                     VLAGQCENSYSCVYLNTLAWRSPTVPLPTENNPRVVFERLFGSGGTNEQRATLARQNR
                     SILDAVTDDFARLTSKLGANDRVQVTEYLDAVREVERRIQTVEKLGDEAGLPALERPR
                     GIPERFDEHVNLMFELQWLAFQADLTRVVTFMLGRDLNFRTYPEVGINRGHHTLSHHL
                     DRPDLLEEYAKLNTYQAQLFAAFLDKLAATPEGDGNLLEHSLFLYGAAMSNPNMHAHF
                     DLPIALVGGASGRHRGGRHLVYPRNTPMTNLLVSMLDKVDVPVEQLGDSSGPLSDV"
     gene            complement(1911..4367)
                     /locus_tag="VM692_15815"
     CDS             complement(1911..4367)
                     /locus_tag="VM692_15815"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HUQ53695.1"
                     /translation="MSRALAVLPLACLCAALVSAGCTPSPAELAANNRALVSRFCFDC
                     HNDLERTGDLSLEAQDFTAVAAHADVWEKVVRKLRAGMMPPQDGGQPLDHERRAALVG
                     WLEGELDRAAAESPNPGRTVPFHRLNRSEYRNAVRDLLALDVDMAELLPADESSYGFD
                     NIAGVLKLSPSLLERYIEAADKVSRLAVGTPSPFASIDYFRVPDDRSQERRLPNLPFG
                     TRGGIAIEHTFPRDGDYVISAELQRTLNEGMPVYTEAQDLEISVDGERLGLFTLPAAT
                     LVPLPPATDEVDISSEIPPPRRTTPDEQRERNRADQDWEVRVPIKAGPHRVVVTFLAK
                     AAALPETVRMPFERPYPLGLNIPEGRSGSYLRSVEISGPYDTTGPGDTASRARLFTCR
                     PSDEGTATDDAERCASEILAGVARRAYRRPVEDADVESLLSFYRLGYEEGGFDFGIQL
                     ALKRLLVNPEFLFRFEPDPTEVTPGETYRIRGTELASRLSFFLWSSIPDDELLAAAER
                     GALETPGELERQVERMLADPKADAFVENFAGQWLFLRNLNAVIPVQTVFPDFDDTLRE
                     GLRRETELFFASIVRENRSVLDLLRADYTYLNERVARHYGIPNIKGAHFRRVTLPADS
                     GRRGLLGHGSILAATSLPNRTSPVVRGKWILENLLGTPPPPPPANVPPLGETDGAGTP
                     LSMRERLAAHRSNPTCASCHTIMDPLGFALENFDAVGAWRTLDDSGAQIDASGALPDG
                     TPFAGLEEFRESLLSSELFVQTLTEKMLTYALGRGVEPYDMPAVRRIVRDAADEDYRF
                     TSFILGVVESTPFTMRRAGS"
BASE COUNT          721 a         1454 c         1518 g          756 t
ORIGIN      
        1 gcatcgcgcg cggcgccggc ggcgagcagg gccttgacgg ccgcgccgtt gttgtcggca
       61 gccgcccaca tgagcgccgt ctggcctttg cgttcctcga cggcattgac gtcggcgccg
      121 gccgcaatca gcgcctcgag cgtggccacg tcacccgtgc gagcggcgat catgagcgcc
      181 gtttcgccct cgactcgcgc accgttcgga tccgcgccgg cctcgagcaa cgcgcgaacc
      241 atcgacgcgt cgccgttctc cgccgcgagg tacaacggcc ggacgccgta gcgattcggt
      301 gcatcgacgg ctgctcccgc gctcagcagc gcggcgaccg tctccgattg gccttggtgc
      361 acggcccagt gcaacgccgc ggtcccgtcg ctgtcggctt cgttcggatc ggcgccgtcg
      421 gccaagaggc cgcgaagccc ggcaccgtcg ttgtccttca cggcctcgag cacggcggcg
      481 ttaccggccg cgcccgcgcc gagcacgatc gttgcgacga gcaacgcccg ggcggccagc
      541 gtcgcgcgtg ccgcggttcg gtcgttatta cacatcggag agtgggcccg agctgtcgcc
      601 gagctgctcg acgggcacgt cgaccttgtc gagcatgctc acgagcaaat ttgtcatcgg
      661 cgtattgcgt ggatagacga ggtgccgccc gccgcgatgc cggccgctcg cgccgccgac
      721 cagcgcaatc ggcaagtcga agtgcgcgtg catgttcggg ttgctcatgg ctgcgccgta
      781 gaggaacagc gagtgctcga gcagattgcc gtcaccctcg ggcgtggcgg cgagcttatc
      841 gaggaaagcc gcgaagagct gcgcctgata cgtgttgagc ttcgcgtatt cctcgagcag
      901 atcaggccgg tcgaggtggt gcgacagcgt gtggtgaccg cggttgatgc cgacctcggg
      961 gtacgtccgg aagttcaggt cccgcccgag catgaacgta acgacgcgcg tcagatcggc
     1021 ctggaacgcg agccattgga gctcgaacat caagttgacg tgctcgtcga accgctccgg
     1081 tatgccgcgt ggccgctcga gcgccggcaa tccggcctcg tcgccgagct tctcgacggt
     1141 ctgaatgcgc cgctcgacct cgcggaccgc gtcgagatac tcggtgacct gcacgcgatc
     1201 gttcgctccg agcttgctcg tcaaacgcgc gaagtcgtcg gtgacggcgt cgaggatgct
     1261 gcggttttgc cgcgccagag tggcgcgttg ctcgttcgtg ccgccgctgc cgaacaaccg
     1321 ttcgaacacg acgcgcggat tgttctcggt cggtagcggc acggtcggcg agcgccacgc
     1381 cagcgtattc aggtagacgc agctgtagct gttctcgcat tgaccggcca gcacgctcaa
     1441 gtcgatcgag agctcgagcg agcgcagcac gctgtcctta ccgatcacgt cggcggcgag
     1501 ctgatcggtg gagacgccgt tctcgaggtc ggcgccctcg gtgcgcttgg ggcgtacgcc
     1561 cgagagccac gacgccgttg ctttggtgtg atcgcccgtc gcgctttctt cgctggcttc
     1621 cgccgggcgc tgcgcgagcc ccgtgaccac cgtcagatgc tcgcgatggc cagcgagcgg
     1681 cgtgaggatt tgcgagagct cgaagttgcg cccttcgcct ttcgggatcc agttgttcac
     1741 gccgaggaag ttcatcgaca cgccattggg caggtagatg aagccaagtc gcgtcacctt
     1801 cggcgcgtcg gcggcgaatg cagggaccat tgcgtcgagc aacggcaggc cgatagcggc
     1861 gccggagccg cgcaagaacg tgcgcctcga cagagctcgt ttcgttacga tcacgatccc
     1921 gctctcctca tcgtgaatgg cgtgctctcg acgacgccga gaatgaaact cgtgaaccga
     1981 tagtcttcgt cagccgcgtc gcggacgatc ctgcgcacgg ccggcatgtc gtacggctcg
     2041 acgccgcggc cgagcgcata ggtcaacatt ttctcggtca gggtttgcac gaacagctcc
     2101 gacgacagca acgattcgcg gaactcttcg aggccggcaa acggcgtgcc gtccggcagc
     2161 gcgccggagg cgtcgatctg cgcaccggaa tcgtcgagcg tgcgccaagc gccgacggca
     2221 tcgaaatttt ccagagcgaa accgagcggg tccatgatcg tgtgacacga cgcgcaggtc
     2281 ggattgctgc gatgcgccgc gagccgctcg cgcatcgata gcggcgtgcc cgcgccgtcc
     2341 gtctcgccga gcggcggcac gttagcggga ggcggcggcg gcggcgtgcc gagcaggttc
     2401 tcgagaatcc acttgccgcg cacgacgggt gacgtgcgat tcggcagcga cgtcgccgcg
     2461 agaatgctgc cgtgaccaag taggccgcgg cggccgctgt cggccggcaa cgtcacgcgg
     2521 cggaagtgcg cgcccttgat gttcggtatg ccgtagtgcc tggccacgcg ctcgttgagg
     2581 tacgtgtaat cggcgcgcaa gagatcgagc acgctgcggt tctcgcgcac aatgctcgcg
     2641 aagaacagct ccgtctcgcg gcgcaagcct tcgcgcaacg tgtcgtcgaa gtccggaaag
     2701 acggtctgca ccggtattac ggcgttcaag ttgcgcagga acagccattg acccgcgaag
     2761 ttctcgacga acgcatcggc tttcggatcg gcgagcatgc gctcgacttg ccgctcgagc
     2821 tcgcccggcg tctccagcgc accgcgctcg gcggcggcga gcagctcgtc gtcggggatg
     2881 ctgctccaga ggaagaacga caggcgcgac gcgagctcgg tgccgcggat gcgataggtc
     2941 tcgccgggcg tcacctcagt cggatcgggc tcgaagcgga acaaaaattc cgggttgacg
     3001 agcaggcgct tcagcgccag ctggatgccg aagtcgaagc caccttcctc gtagccgaga
     3061 cgataaaaac tcagtagcga ctcgacatcg gcgtcctcta ccgggcggcg ataggcgcgt
     3121 cgcgctacgc ccgcgagaat ctcggacgcg cagcgctctg cgtcgtccgt cgccgtcccc
     3181 tcatcgctag gccgacacgt aaacaggcgc gctcggctcg ccgtgtcgcc tgggcccgtc
     3241 gtgtcgtacg ggccgctgat ctcgacgctg cgcagatacg agcccgagcg cccctctgga
     3301 atgttgaggc cgagcggata cgggcgctcg aacggcattc gcaccgtctc tggcagcgcc
     3361 gctgctttag cgaggaacgt tacgacgacg cggtgcggac ctgccttgat cggcacgcgc
     3421 acttcccagt cctggtccgc gcgattgcgc tcgcgttgct cgtcgggcgt cgtgcggcgc
     3481 ggcggcggaa tctcgctgct gatgtcgact tcgtccgtgg ccgggggcag cggcacgagc
     3541 gtcgccgccg gcaacgtgaa caggccgagc cgctcgccgt cgacgctgat ctcgaggtcc
     3601 tgcgcttcgg tgtagaccgg catgccctcg ttcaacgtgc gctgtagctc ggccgagatg
     3661 acgtagtcac cgtcgcgcgg gaacgtgtgc tcgatggcaa tgccgccgcg cgtaccgaac
     3721 ggcaagttcg gcaagcggcg ctcctgcgag cggtcgtcgg gcacgcggaa gtaatcgatg
     3781 ctcgcgaacg gcgacggagt gccgaccgcg agccggctca ccttgtcggc agcctcgatg
     3841 tagcgctcga gcaagctcgg cgaaagcttc agcacgccgg cgatgttgtc gaagccgtag
     3901 ctcgactcgt cggccggcaa cagctcggcc atgtcgacgt cgagcgccaa aaggtcgcgc
     3961 accgcattgc ggtattcgct gcggttcagc cggtggaacg gcacggtgcg gccgggattc
     4021 ggtgactcgg cggcggcgcg atcgagctcg ccttcgagcc aaccgacgag cgccgcacgt
     4081 cgctcgtggt cgagcggctg tccgccgtcc tgcggcggca tcatgccggc gcgcaacttg
     4141 cggacgactt tttcccagac gtcggcgtgc gcggcgacgg cggtgaaatc ttgcgcttcg
     4201 agcgaaagat caccggtgcg ttcgagatcg ttatggcagt cgaaacaaaa tcgggaaacg
     4261 agggcccggt tgttcgccgc aagctccgcg ggcgacggcg tgcagcctgc cgacacgagc
     4321 gcggcgcaga ggcacgcgag aggtaggacc gcaagtgctc gcgacaaacc gtaacctcga
     4381 ttgcaacgtc gcactatctt acaaatgtag cgatacggcg ggctccggtc gactttaatt
     4441 atcgctggt
//
LOCUS       DATLEE010000721         6151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11066608, whole genome shotgun sequence.
ACCESSION   DATLEE010000721 DATLEE010000000
VERSION     DATLEE010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11066608"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1822
                     /locus_tag="VM692_15820"
     CDS             <1..1822
                     /locus_tag="VM692_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HUQ53696.1"
                     /translation="GQVALTVIAADHVDLAVALSVAPTPVELETAVTWTVAIQNLAAG
                     VAAPGMSLEASFVGDVPFRFDAPTTPGCTLTPSGNDTRLSCTLGPLAGGASTTITLTG
                     RGSFAGDVFGRATVAPTGAAVDGVPGNVTTTASLSLAQRVSRAPEQSIAGLAAVAVAA
                     ADFNGDGFDDLAVATASPLGVVLLANVVDPANAARRIIATTPQSLGGEALGTDLAVAD
                     LDRDGDLDLVLAAAAGAPDRAFLAANGSFTSTGLGDPAVDSRAVAAADVNGDAFVDLV
                     FASPGGSRLLINSGSGGVFTSGPRIGAGDARDVLLVDLSGDGLPELVVANADGDAAIY
                     GNTAGAFTLARTLPTGPTTAVAAGDFNGDDRSDLVFTRATAEPPKVPSALVWLNGPEL
                     FLSDELGAAAASGLVVRDFNLDGTDDVLASSSTGQRLFTNAGAANGTFVLHPQQLATP
                     GARGVAAGRFSNDERVDLAVAGDGIAVFVNDGKGNLGSGDTTPPTLTLRGEPTVNIVI
                     DSPFTDPGAAASDAIDGDLSSRVVVSGSVNTAVLGTYTLNYAVSDLSGNAATPVTRTV
                     NVQAQPGASDGGGGGAVGMELVALLLAAALARLRRPGRAR"
     gene            1918..2304
                     /locus_tag="VM692_15825"
     CDS             1918..2304
                     /locus_tag="VM692_15825"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53697.1"
                     /translation="MTRNFPAVLALMALTAPALGHHSETAEFDQNVPVKVTGTIVKVE
                     WLNPHVWFYVDVPDDKGNVTTWGFSGAPPGSLMRRGITKDVLKIGAVVNVEGSRARDG
                     SNNASGRSVTFPDGRNVFTATQEDAR"
     gene            2346..3080
                     /locus_tag="VM692_15830"
     CDS             2346..3080
                     /locus_tag="VM692_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53698.1"
                     /translation="MLGAAASSSAQSVPRMADGKPDFSGIYSPPATVNATGPRGNLIF
                     NADKMAPVKPGAEGLLYEARNGDPRHDEPRAMCLPAGFPSGMLYILPIQIVQNPKNIV
                     IIPELQRAARIIPTDGRPHREGIEPSYYGDSVGKWEGDTLVIDTVNFKRWILDDYHYT
                     DPTKTRWHSDALHTTERLTRDGDKLRYQITIDDPKIFTRPFSQDFELTLRPDWEPLGL
                     LEYVCEENNRCAGGNCRPSEAQQASP"
     gene            complement(3280..4071)
                     /locus_tag="VM692_15835"
     CDS             complement(3280..4071)
                     /locus_tag="VM692_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53699.1"
                     /translation="MHNSVTPRTTLPFVNLLACLLAAPVCAQDDASTKAGPAPRLADG
                     HPDLSGVWWGGADIGGRGARLGGGPRDPNAVTYTSLYQPWAADKSKTLSDKDDPTLRC
                     VPTAFGTLNVRLWNVGAVGQIVATPKLVVLLSETYHDYQLVPTDGRPHRDDQPPTYRG
                     DAVGRWDGDTFVVDVTNFTDDTWISAEGRVSIHSDALHVVERYRRVDAETLEIEATIE
                     DPKALTGPWQVPKQTLKLAPFDTLLTLDCASVEADAMIRSASQPK"
     gene            complement(4087..4494)
                     /locus_tag="VM692_15840"
     CDS             complement(4087..4494)
                     /locus_tag="VM692_15840"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53700.1"
                     /translation="MNDKVWTSLAGAALSLAASTAAAHHSFAAVYNMQQPITVKGTIV
                     QVRLTNPHSWFYLDVKNDAGEVERWAFEAGTPSGMIRNGYKASEIKEGTEVTITGFQA
                     RDASQHMGMLRQLVMADGTVYGLFGPQEGPSAR"
     gene            complement(4581..4934)
                     /locus_tag="VM692_15845"
     CDS             complement(4581..4934)
                     /locus_tag="VM692_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53701.1"
                     /translation="MTEHDIDSDGVITRFEAQAQTQALNVDADELLTEIRRGDLLSAI
                     EKRETADSAYTATDYRRELDGLTDVEVIDLANLSGAAFEARRQANAQKFRSIHCWHQD
                     RPDRSKSRITLRRCS"
     gene            complement(5140..>6151)
                     /locus_tag="VM692_15850"
     CDS             complement(5140..>6151)
                     /locus_tag="VM692_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ53702.1"
                     /translation="VLKPSADGASVASSTVFATGLTRPFGIQFYPAGATPQWLYVAQE
                     NRVVRFRYNSGDGKATGEPEVVVSQLASTTGGHYTRDLVFSPDGRRMFVAVGSLSNVA
                     EDMPKKSADEIKTWEGAHGTGAAWGQEENRADVLVFDVGANQPGKPFATGIRNCVALA
                     IQPGTGDLWCTVNERDALGDNLVPDYSTRVREGGFYGWPWYYMGDKEDPRHAGERADL
                     KGKTITPDVPYEAHSAAVGFTFYTATSGRSAFPADYVGDAFAVFHGSWNRADRTGHKV
                     VRVPIENGVPTGEYVDFLVGFVTPDNKPWGRPSYITAMNDGSLLLGDDDGDVIYRISY
                     AR"
BASE COUNT          992 a         2081 c         2043 g         1035 t
ORIGIN      
        1 ggggcaggtt gcgctgacgg tcattgcagc ggaccacgtc gatctcgccg tggctttgag
       61 cgttgcgccg acccccgtgg agctggaaac ggccgtgacg tggaccgtcg cgatccaaaa
      121 tttggcggcc ggcgtcgccg cgcccggtat gtcgctcgag gcgtcgttcg tcggcgacgt
      181 cccgttccgc ttcgacgcac cgacaacgcc gggctgcact ctgacgcctt cgggcaacga
      241 cacgcgtttg agctgcacgc tggggccgct cgcgggcgga gccagcacga cgatcacatt
      301 gacgggccgc ggcagcttcg ccggcgacgt cttcggccgc gcgaccgtag cgccgacggg
      361 cgccgccgtc gacggcgtgc ccggcaacgt tacgaccacg gcgtccctga gtctggcgca
      421 gcgcgtcagc cgcgcgccgg agcagagcat tgccggcctc gcggctgtcg ccgtcgcggc
      481 cgccgacttc aacggcgacg gcttcgacga cctcgccgtg gcgacggcgt cgccgctcgg
      541 cgtcgtgttg ctcgcgaacg tcgtcgaccc cgccaacgcg gcgcgacgca taatcgcgac
      601 gacgccgcaa tcgctcggcg gcgaagcgct cgggaccgac ttggccgtcg ccgatctcga
      661 ccgcgacggc gacctcgatc tcgtcctcgc tgcggctgcc ggcgcgcccg atcgtgcctt
      721 cctcgccgcc aacggatcgt tcacgagcac ggggctcggc gaccccgctg tcgacagccg
      781 cgccgtcgcg gccgccgacg tgaacggcga tgcgttcgtc gacctggtgt tcgcgagccc
      841 cggcggcagc cggttgttga tcaacagcgg ctccggcggc gtcttcacga gcgggccgcg
      901 aatcggcgcc ggtgacgcgc gcgacgtgct gctcgtcgac ttgagcggcg acggattgcc
      961 ggagctcgtc gtcgcgaatg ccgacggcga cgccgccatc tacggcaaca cggccggcgc
     1021 cttcacgctc gcgcgcacgc tgccgactgg gcccacaaca gctgtcgctg ccggcgactt
     1081 caatggcgac gaccgctccg acctcgtgtt cacgcgagcg acggcggagc cgcccaaagt
     1141 gccgagcgcg ctcgtctggc tcaacggccc cgagctcttt ctctcggacg agctcggcgc
     1201 ggccgccgca agcggcctgg tcgtgcgcga tttcaacctc gacggcacgg acgacgtcct
     1261 cgcgtcgagc tcgacgggtc aacgcctctt cacgaacgcg ggcgccgcga acggcacgtt
     1321 cgtgctccac ccgcagcagc tcgcgacccc tggcgcgcgc ggcgtcgctg cgggtcggtt
     1381 cagcaatgac gagcgcgtcg atctcgcggt ggccggcgac ggcatcgccg tcttcgtcaa
     1441 cgacggtaag ggtaatctcg gctccggcga tacgacgccg ccgacgctga cgctgcgcgg
     1501 cgagccgacc gtcaacatcg tcatcgattc gccgttcacg gatcccgggg ctgccgcgtc
     1561 ggacgcaatc gacggcgact tgagctcgcg cgtcgtcgtg tcgggcagcg tcaatacggc
     1621 tgtgctcggc acgtatacgc tgaactacgc cgtcagcgat ctctcgggca acgctgcgac
     1681 acccgtgacg cgcacggtca acgtccaagc gcagcccggc gcgtccgatg gcggaggcgg
     1741 cggcgcggtg ggcatggagc tggtcgcgct gttgctggcc gcagcgctgg cgcgcctgcg
     1801 gcggcccggc cgcgcgcgct gaagacggcc gctatactgg ctcccattgc tacgagtctt
     1861 gcgttttgtg ccccagttgt ggggacatac gccttggtat cgttgcggag gcaggcgatg
     1921 acgcgaaatt tcccggccgt cttggcgcta atggctctta cggcaccggc cctcggccat
     1981 cactccgaaa ccgcggagtt cgaccagaac gttccggtca aagtgacggg caccatcgtc
     2041 aaggtcgagt ggctgaaccc gcacgtctgg ttctacgtcg acgtgccgga cgacaagggc
     2101 aacgtcacga cgtgggggtt ctcgggagcg ccgccgggct cgctgatgcg acgcggcatc
     2161 acgaaggacg tcttgaagat cggcgccgtc gtcaacgtcg agggttctcg cgcacgcgac
     2221 ggctccaaca acgcttccgg ccgcagcgtg acgttccccg acggcaggaa cgtgttcacg
     2281 gcgacacaag aggacgcgcg atgaaaacgg cgaggctggt atgaagcatc gttgcggcag
     2341 cgctcgtgct gggggcggct gcgagctcga gcgcgcagag cgtgccgcgc atggcggacg
     2401 gcaagccgga tttttccggc atctactcgc cgcctgcgac ggtgaacgcg acggggccgc
     2461 gcggcaatct gatcttcaac gcagacaaga tggcgcccgt gaagccgggc gcggaagggt
     2521 tgctgtacga ggcccgcaac ggcgatccgc ggcacgacga gccgcgtgcg atgtgcctgc
     2581 cggccggctt tccgtccggc atgctgtaca tcctgccgat ccaaatcgtg cagaacccga
     2641 agaacatcgt gatcattccc gagctacagc gcgcggcgcg catcattccg accgatggcc
     2701 gcccgcaccg cgagggcatc gagccctcgt actacggcga ctccgtaggg aagtgggagg
     2761 gcgacacgct cgtcatcgac acggtgaact tcaagcgctg gattctcgac gactatcact
     2821 acacggaccc gacgaaaacg cgttggcaca gcgacgcgct gcatacaacg gaacgtctga
     2881 cgcgtgacgg cgacaagctg cgctaccaga tcacgatcga cgaccccaag atcttcacgc
     2941 ggcccttctc gcaggacttc gagctgacct tgcgcccgga ctgggagccg ctcggcctgc
     3001 tcgagtacgt gtgcgaggag aacaatcgct gcgccggcgg caactgccgt ccgtctgaag
     3061 cgcagcaagc ttcgccttag cgggcctgtc taagcgccgc cgaacgcaac gcgaaggacg
     3121 gcggcgacgc tgaatagaac aatcagcatc actgctagac caagcaacgc cgccgccggt
     3181 cgcgggcatg agccttctgc ggtggtgagt gggcgcacgt tcatggccca acgatagggc
     3241 gctgttcctg tacgcgagct gaacgtacgg ggcgagcgac tacttgggct gactcgcgct
     3301 gcgaatcatc gcgtcggctt cgaccgacgc gcaatcgagc gtcagcagcg tgtcgaacgg
     3361 cgcgagcttc agcgtttgct ttggcacttg ccacggcccc gtgagcgctt tcgggtcctc
     3421 gatcgtcgcc tcgatctcga gcgtctcggc gtcgacgcgt cggtaacgct cgaccacgtg
     3481 cagcgcgtcc gaatggatcg agactcggcc ctcggccgag atccaggtgt cgtccgtgaa
     3541 attagtgacg tcgacgacga acgtgtcgcc gtcccaacgt ccgacggcat cgcctcgata
     3601 cgtcggcggc tggtcgtcgc gatgaggcct cccgtccgtc gggacgagct ggtagtcgtg
     3661 atacgtttcc gacagcagca cgacgagctt cggagtcgcg acgatctggc cgacggcgcc
     3721 gacgttccag agccgcacgt tcagcgtgcc gaatgccgtc ggcacgcagc gcagcgtggg
     3781 gtcgtccttg tcgctcaggg tcttcgactt gtcggcagcc cacggctggt acaggctcgt
     3841 gtaggtcacg gcattcgggt cgcgcggccc gccgcccaag cgtgcgccgc ggccgccgat
     3901 gtccgcgccg ccccaccaga cacccgacaa atcgggatga ccgtcggcga ggcgtggcgc
     3961 cggcccggcc ttcgtgctcg cgtcgtcctg tgcgcaaacg ggcgccgcca gcaggcatgc
     4021 gagcaggttg acgaagggca gagtggtccg tggcgtgact gagttgtgca tcgaaaatcc
     4081 tttgagctag cgcgcgctcg ggccttcctg cggcccgaac aagccataca cggtgccgtc
     4141 cgccatgacg agttgacgca gcatccccat gtgttgcgac gcgtcccgcg cttggaagcc
     4201 cgtgatggtg acctcggtgc cctccttgat ctcgctggct ttgtagccgt tgcggatcat
     4261 gccgctcggt gtcccggctt caaacgccca ccgctccact tcgccggcgt cgttcttcac
     4321 gtcgaggtaa aaccacgagt gcggattcgt gaggcgcacc tgaacgatcg tgcccttgac
     4381 cgtgatcggc tgttgcatgt tgtagaccgc ggcgaacgag tggtgcgccg cagctgtgga
     4441 cgcggcgagc gacagcgccg cccccgcaag cgacgtccat actttgtcat tcatcgagaa
     4501 gcctccacga atcagtcgtt caattctccg tgcagcggca gcgcgttgca aacacgccga
     4561 ccgtgcgcgc ctgcagcccg tcaggagcag cggcgtagag tgattcgcga cttcgacctg
     4621 tcggggcgat cctgatgcca acaatggatg ctgcgaaatt tttgcgcgtt tgcttgccga
     4681 cgagcttcga aagcggcgcc gctgaggttg gcaagatcga taacctcgac gtccgtaagc
     4741 ccgtccagtt cccgtcgata gtcggtcgct gtgtaagcgc tgtccgccgt ctcgcgcttt
     4801 tcgatcgcgg atagcaaatc gccgcgccga atctctgtca gcagctcatc ggcgtccacg
     4861 tttaacgctt gcgtttgggc ctgcgcttcg aaccgcgtga tgacgccatc cgagtcgatg
     4921 tcgtgctcgg tcaaaggcgt gttccgcagc cagagcacgc cgagggcgac ctcgagctgt
     4981 tcgcgccgtc gagccggcaa actgttttgc agcagcacga tcgatctctc gaagctcgct
     5041 gcgctgcttg catcgatgcg cgagaccggc gcttcgatcg acccagcgca tcccgccagt
     5101 ccgacacaca tgatcaacgt gagccgggcc atgtcgccgt taacgtgcgt acgagatgcg
     5161 gtagatgacg tcgccgtcgt cgtcgccgag cagcaacgag ccgtcgttca tggcggtgat
     5221 gtaggagggc cggccccacg gtttgttgtc cggcgtaacg aagccgacga ggaaatcgac
     5281 gtactcgccc gtcggcacgc cgttctcgat cggcacgcgc acgactttgt gacccgtgcg
     5341 gtcggcgcgg ttccacgagc cgtggaacac ggcgaacgcg tcgccgacgt agtcggccgg
     5401 aaacgccgag cgccccgaag tagccgtgta gaacgtgaag ccgacggcgg ccgaatgcgc
     5461 ttcgtacggc acgtcgggcg tgatcgtctt gcccttcaag tccgcgcgtt cgccggcgtg
     5521 ccgcggatcc tctttgtcgc ccatgtaata ccagggccag ccgtagaagc cgccttcgcg
     5581 cacgcgcgtc gagtaatcgg gaacgaggtt gtcaccgagc gcgtcgcgct cgttgacggt
     5641 gcaccaaaga tcgcccgtgc cgggctggat cgcgagcgcc acgcagttgc ggatgccggt
     5701 cgcgaaaggc ttgccgggct gattcgcgcc gacgtcgaac acgagcacgt cggcccggtt
     5761 ctcctcctgc ccccaagccg cgccggtgcc gtgcgcgcct tcccacgtct tgatctcgtc
     5821 ggccgatttc ttcggcatgt cctctgccac gttcgacagc gagccgacgg cgacgaacat
     5881 gcgccggccg tccggcgaga acacgagatc gcgcgtgtag tggcctccgg tcgtggacgc
     5941 gagctggctc acgacgacct cgggctcacc cgtggccttg ccgtcaccag agttgtagcg
     6001 gaaacggacg acgcgattct cttgcgccac gtacagccac tgcggagtcg cgccggcagg
     6061 gtagaactgt atgccgaacg gccgcgtcag gccggtcgcg aacacggtgg agctcgcgac
     6121 gctcgcgccg tcggcggaag gcttcagcac c
//
LOCUS       DATLEE010000722         3279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8840270, whole genome shotgun sequence.
ACCESSION   DATLEE010000722 DATLEE010000000
VERSION     DATLEE010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8840270"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VM692_15855"
     CDS             complement(<1..1295)
                     /locus_tag="VM692_15855"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HUQ53703.1"
                     /translation="MSRVVKRVVVMGGGTSGWMSACYIERLLARMQVPDASVTLVESE
                     DIGIIGVGEATLNDLKRTLRFLEIDEREFMIRTHATFKNGIKFVNWREDPAKVGRHHY
                     YHPFEAVPKIDGMWLVDVWSCLKADNDIHLDYGYMTGPSAFISDKLRSPKTADSKPYE
                     GLLDYAYHTDTVLFGRYLREVALSRGVKRIVDNVVGVQSNERGLITALNTKEHGAIEG
                     DLFIDCSGFGGYLINKHFGTEFVSFNDSLFNDRAVALRVPYRSADAPINPFTTATAMS
                     SGWIWDIPLSDQRVELGSHQRWSDDSEHGRRGTGYVYSSQFISEEAAERELRGYVGEQ
                     AEGLNARHLKMRIGHNRKFWVKNCVAIGLAGGFIEPLESTGIGLISQGLALLAFHFPT
                     RDFPEPLAESYNGAMTRLYEHIREFIVLHYCTTRREDTE"
     gene            complement(1297..2070)
                     /locus_tag="VM692_15860"
     CDS             complement(1297..2070)
                     /locus_tag="VM692_15860"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HUQ53704.1"
                     /translation="MHNPDSVADEPMRFNAGLDVGAAARSFAQCGRVRIDDVLDALSA
                     ARLHRCLVEDVPWQLTYNEGEKNVFLDAAALQELGERKQHELLQRVLVRAQSQFQYLF
                     RSYPMVKAYVENSEPGLLLHAVFEWLNDPRTLAVLRKITGIESLLKVNAQATLYRPGH
                     FLSRHDDSGYPEQHRRVAYVLYMTRGWSADWGGQLQFLDAQGEVEEVWMPRFNSLGLF
                     LVPMPHVVGYVAPFATQPRFAITGWLCDGPATPATAVRT"
     gene            complement(2091..3146)
                     /locus_tag="VM692_15865"
     CDS             complement(2091..3146)
                     /locus_tag="VM692_15865"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HUQ53705.1"
                     /translation="MSAQLSANVTEAARCDSSGNHARAVSLLAEASAQGDVEAMTRLG
                     KRLLVAANAPDRPKEGTELIRAAAELGGAEAAAQLAVLSAIGMFVNQSWEAALAALTF
                     AAERGWPAARRQLRILTADRDLAAQPQLDSAAWRRLAGTIDLAQWHAPAAGATLSESP
                     LVRHFPAFASAEVCEWLIEKSRGRLVRAPVYSQATRTEAISETRTNTWAMFNVLEADL
                     VSVLVQVRMCATTGLAFRYLEPLSVLHYAVGEEITEHFDFVDPARTPNYDQELRENGQ
                     RVVTFLIYLNDDYADGKTEMAAARLSHKGQRGEGLYFVNAHANGDPDRRTLHAGRPPT
                     QGEKWIVSQFMRDLPLF"
BASE COUNT          552 a         1088 c         1058 g          581 t
ORIGIN      
        1 aactccgtgt cctcgcggcg cgtcgtgcag tagtgcagca cgatgaactc gcgaatgtgc
       61 tcatagaggc gcgtcatcgc gccgttgtag ctctcggcca gcggctccgg aaaatcgcgc
      121 gtcgggaagt ggaacgccag cagcgcgagc ccttgtgaga tcagcccgat ccccgtcgac
      181 tcgagcggct cgatgaagcc gccggcgagc ccgatcgcga cgcagttctt gacccagaac
      241 ttgcggttgt ggccgatcct cattttcaga tgccgagcgt tgaggccttc ggcctgctcg
      301 ccgacgtagc cgcgcagctc gcgctcggcg gcctcctccg aaatgaattg actcgaatac
      361 acatagccgg tgccgcggcg cccgtgctca ctgtcgtcgc tccaacgctg gtggctgccg
      421 agctcgacgc gctgatcgct gagcgggatg tcccagatcc aaccgctgct catggccgtg
      481 gccgtcgtga acgggttgat cggcgcatcg gccgagcggt acggcacgcg caacgcgacg
      541 gcgcgatcgt tgaacaacga gtcgttgaag ctcacgaact cggtgccgaa gtgcttgttg
      601 atcaggtacc cgccgaagcc ggagcagtcg atgaacagat ctccctcgat ggccccgtgc
      661 tctttggtat tcagcgcggt gatcaaccca cgctcgttgc tctgcactcc gacgacgttg
      721 tcgacgatgc gcttgacgcc gcgactcagc gcgacttcgc gcagatagcg gccgaacagc
      781 acggtgtcgg tgtgatacgc gtaatcgagc aagccctcgt acggcttgct gtcggctgtc
      841 ttcggcgacc gcagcttgtc ggagatgaaa gccgatgggc ccgtcatgta gccgtaatcg
      901 aggtggatat cgttgtcggc tttgagacac gaccagacgt cgacgagcca catgccgtcg
      961 atcttcggca cggcctcgaa cgggtggtag tagtggtgtc ggccgacctt cgcggggtcc
     1021 tcgcgccagt tcacgaactt gatgccgttc ttgaacgtcg cgtgagtccg gatcatgaac
     1081 tcgcgctcgt cgatctcgag aaagcgcagc gtgcgtttga gatcgttgag cgtcgcctcg
     1141 ccgacgccga tgatcccgat gtcctcggac tctaccaagg tgacgctcgc gtccggcacc
     1201 tgcatgcgcg cgagcagccg ctcgatgtag caggcgctca tccagcccga cgtgccgccg
     1261 cccatgacga ccactcgttt gacgacgcgg ctcacgttag gtccgcacgg ccgtagcagg
     1321 cgtggcaggc ccgtcgcaga gccaccccgt gatcgcgaaa cgcggctgcg tcgcgaacgg
     1381 tgcgacgtag ccgacgacgt gcggcatcgg cactaggaac aggcccagcg aattgaagcg
     1441 cggcatccag acctcttcga cctcaccctg cgcatcgagg aactgcagct gcccgcccca
     1501 atccgccgac caaccgcgcg tcatgtacag cacgtaagcg acgcgccgat gctgctctgg
     1561 gtagccgcta tcgtcgtgcc gcgacaggaa atggccgggg cgatacagcg tggcctgggc
     1621 gttcactttg agcaacgatt cgatgccggt aattttccgg agcacggcga gcgtccgagg
     1681 atcgttgagc cactcgaaaa ccgcgtgcag cagcagcccc ggctcgctgt tctcgacgta
     1741 ggccttgacc atcgggtagc tgcggaacag atattggaac tggctttgcg cgcgcaccag
     1801 gacgcgctgc aagagctcgt gctgcttgcg ctcgccgagc tcttgcagtg ccgcggcgtc
     1861 gagaaagacg ttcttctctc cctcgttgta cgtgagctgc catggaacgt cctcgacgag
     1921 gcagcggtgc agccgggcgg cgctaagcgc gtcgaggacg tcgtcgatgc gcaccctgcc
     1981 gcactgcgcg aacgagcgcg cggcggcccc gacgtccagc ccggcgttga agcgcatcgg
     2041 ctcgtcggcg acggaatccg gattgtgcat ggcgagtcgt ccgccgactc tcagaacagc
     2101 ggcaggtcgc gcatgaattg cgacacgatc catttctcgc cttgcgtcgg cgggcggccc
     2161 gcgtgcagcg tgcgccgatc ggggtcgccg ttcgcgtgcg catttacgaa gtagaggccc
     2221 tcgcctctct gccctttgtg cgacaggcgg gcggcagcca tctccgtctt cccgtcggcg
     2281 taatcgtcgt tcaaatagat caagaacgtc acgacacgtt gaccgttctc gcgcagctcc
     2341 tggtcgtaat tgggggttct cgcaggatcg acgaagtcga agtgctccgt gatctcttcg
     2401 ccgaccgcgt agtgcaggac ggacaacggc tcgaggtagc gaaacgccaa gcccgtcgtc
     2461 gcgcacatgc gtacctgcac gagcacactg acgaggtcgg cttcgagcac gttgaacatc
     2521 gcccacgtgt tcgtgcgcgt ttcgctgatc gcctccgtcc gcgtcgcttg actgtacacg
     2581 ggagcgcgca cgagccgacc gcgcgacttt tcgatcagcc attcgcagac ttcggcgctc
     2641 gcgaaagcag ggaaatgccg aacgagcggc gattcgctca aggtggcgcc ggccgccggc
     2701 gcgtgccact gcgcgagatc gatcgtgccg gcgagccgcc gccatgctgc cgagtcgagt
     2761 tgcggctgcg cggccaggtc gcgatcggcg gtgagtattc gcagctgtcg ccgagccgcc
     2821 ggccaaccgc gctcggccgc gaacgtcagc gcagcgagcg ctgcctccca gctctggttc
     2881 acgaacatcc cgatcgccga caacaccgcg agctgcgccg cggcctcggc accgccgagc
     2941 tcggcggcgg ctctgatgag ctcggtgcct tccttgggac ggtcgggcgc attcgccgcg
     3001 acgagcaggc gtttgccgag gcgcgtcatc gcctcgacgt cgccctgggc actcgcctcc
     3061 gccagcagac tcaccgcgcg cgcgtggttg ccgctcgaat cgcagcgggc cgcctcggtg
     3121 acgttcgcgg acagttgagc actcatgcgc gcattggctc tagggccatt ccctgcccgc
     3181 taacctagcg cgaattggag cagcggtcca agccggacgg agacggcggc gggccgaccc
     3241 cgccgccgcg caagcaagcc cggttcagaa gctcatctt
//
LOCUS       DATLEE010000723         2118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7349842, whole genome shotgun sequence.
ACCESSION   DATLEE010000723 DATLEE010000000
VERSION     DATLEE010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7349842"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VM692_15870"
     CDS             <1..364
                     /locus_tag="VM692_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53706.1"
                     /translation="VLFPAAGLDAGQVHDFYEVMELVEKLSYAGNEAARRYLDGRIDG
                     AAARRWLETYTLSAPERAAQRVRFFDQYRAYVINYNLGKDLVAAHVERLAGNDAERRW
                     QEFARLISSPRLPSGLQK"
     gene            412..1512
                     /locus_tag="VM692_15875"
     CDS             412..1512
                     /locus_tag="VM692_15875"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUQ53707.1"
                     /translation="MRFTSAEWARSLAVTIVAVAAAWHCTAQPLPIEDIQLRGDRFRG
                     LHYREMTEEQRALLYNVVNSARAVTNTTGNGPFNTLLRTPQVGELSQQLGNAIRFSSG
                     LSGRLRELATLMAGRAWTSRYEWYAHARYALDEGIEPAVVAAIAAHARPELAAMPAED
                     AAVWRVADELLYTRRITDATYKEALAVLGERRLFSTVTIAAYYEYVSMLLNVDRYPLP
                     ADAPADAAAAARGLVPLTATELRRRQTHGAVTARPVIRNDDTGQALDALRAAVTRPEE
                     LPADVRLVAASAVVQHWRGDSEAFATRSVLADTAYRFAAELLDTRFVSDALFAEALKA
                     LGERGVVNLMVFMGQSNIRCAQQALAGAACAL"
     gene            1535..>2118
                     /locus_tag="VM692_15880"
     CDS             1535..>2118
                     /locus_tag="VM692_15880"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUQ53708.1"
                     /translation="MKLRRLPLASTALCLLAAAGVAGAQSLPSDLDAKSRARVPYLVR
                     KDAGEDAKRLWDIFVRNSNSPTDTLGGPLAFSAYNTPVAIALLDLHDGAVGKSSSLDA
                     HVRELAILVACRATNYSFEFNGHEAAAVKAGVDQKVIDIVRTNGALTGLPDADAAVIR
                     FGRELFIDRKMSSATFAKAKQLYGDKGALDLVAV"
BASE COUNT          342 a          716 c          708 g          352 t
ORIGIN      
        1 cgtgctgttt ccggccgcgg gactcgacgc gggccaagtc cacgacttct acgaggttat
       61 ggagctcgtc gagaagctct cgtacgccgg caacgaagcg gcgcgccgct acctcgatgg
      121 gcgcatcgac ggcgcggccg cgcggcgttg gctcgagact tatacgttga gcgcaccgga
      181 gcgcgccgcg cagcgcgtgc ggttcttcga ccaataccgc gcgtacgtca tcaattacaa
      241 cctcggcaag gatctcgtgg ctgcgcacgt cgagcggctt gcaggtaacg atgccgagcg
      301 gcgctggcag gagtttgcgc gcctgatctc gtcgccgcgc ctgccgtccg gtcttcaaaa
      361 gtaggcacgt cggcctatac tgagcccgcg tgatctgaaa gcgggagcag ggtgcgattc
      421 acaagcgctg aatgggctcg gagcctcgcg gtaacgatcg ttgccgtcgc ggccgcgtgg
      481 cactgcaccg cgcagccgtt gccgatcgag gacatacagc tgcgcggcga ccgcttccgc
      541 ggcttgcact accgcgagat gaccgaagag cagcgcgcgc tgctctacaa cgtcgtcaac
      601 agcgctcgcg ccgtcacgaa cacgacgggc aacggcccgt tcaacacgtt gctgcgcacg
      661 ccgcaagtcg gcgagctctc gcagcagctc ggcaatgcaa ttcgattttc ctcgggcttg
      721 tcgggacgct tgcgcgagct cgcgacgctg atggcggggc gagcgtggac gtcgcgctac
      781 gagtggtacg cgcatgcacg ctacgcgctt gacgagggca tcgagccggc cgtcgtggcc
      841 gccatcgcgg cgcacgcacg accggagctt gcagccatgc ccgccgagga cgcggccgtc
      901 tggcgcgtcg ccgacgagct tttgtatacg cgccgcatca cggatgcgac ctacaaggaa
      961 gcgctcgcgg tgctcgggga gcggcgcctg ttcagcacgg tgacgatcgc ggcgtactac
     1021 gagtacgtgt cgatgctgtt gaacgtcgat cgttatccgc tgccggccga cgcgccggcc
     1081 gatgctgcgg ccgccgcacg cggcctagtg ccgctcacgg cgaccgagct gcgccgtcgc
     1141 cagacgcacg gcgccgtgac ggcacggccc gtcattcgca acgacgacac ggggcaggcg
     1201 ctcgacgcgc tgcgcgccgc cgtgacgcgc cccgaggagc tgccggccga tgtgcgcctc
     1261 gtggccgcgt cggccgtcgt gcagcactgg cggggcgatt cggaggcttt cgctacgcgg
     1321 tccgtcctcg cggacacggc gtatcgcttt gcggccgagc tgctcgacac gcgcttcgtc
     1381 tccgacgctc tgttcgcgga ggcgctgaag gcgctcggcg agcgcggcgt cgtcaacctc
     1441 atggtgttca tggggcaatc gaacattcgc tgcgcgcagc aggcactcgc cggcgctgcg
     1501 tgcgcgctct agtcacaaca aggagaacct ctcgatgaaa cttcgccgcc tgccactagc
     1561 ttctaccgct ctctgcctgc ttgctgcggc cggcgtcgcc ggcgcccagt cgttgccgag
     1621 cgatctcgac gcgaaatcgc gcgcgcgtgt gccgtacttg gtgcgcaaag acgccggcga
     1681 agacgcgaag cgcctgtggg acatcttcgt ccgcaacagt aacagcccga ccgacacgct
     1741 cggcggcccg ctggccttct cggcctacaa cacgccggtc gcgattgcgc tgttggactt
     1801 gcatgacggc gccgtcggca agagcagctc cctcgacgcg cacgtgcgcg agctcgcgat
     1861 cctcgtcgct tgtcgcgcga cgaattacag cttcgaattc aacggccatg aggccgcagc
     1921 agtaaaagcc ggcgtcgacc agaaagtcat cgacatcgtg cgcacgaacg gcgcgctgac
     1981 gggcctgccc gatgccgacg ctgcagtgat ccgcttcggc cgcgagctct ttattgaccg
     2041 caagatgagc tcggctacgt tcgcgaaggc gaagcagctc tatggagaca agggcgcgtt
     2101 ggatctcgtc gccgtcat
//
LOCUS       DATLEE010000724         5800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7350488, whole genome shotgun sequence.
ACCESSION   DATLEE010000724 DATLEE010000000
VERSION     DATLEE010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7350488"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1904)
                     /locus_tag="VM692_15885"
     CDS             complement(192..1904)
                     /locus_tag="VM692_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HUQ53709.1"
                     /translation="MAGLKDPHGGALKNLYLDEAGAKQEKARARDLASWDLNMRQLCD
                     IEMLLNGAFSPLEGFLDEADYKRVLTEMRLTSGVLWPMPINLDVTEQFASKLKVGDSI
                     ALRDGEGVLTATMQVSSIFRPDFDVEAKAVFGTTDLKHPAVSYLKSRSNPVYLGGKLR
                     GVEAPGHYDFKHLRDTPKELRDRFQKLGWRRIVAFQTRNPMHRAHQELTFRAAKDVEA
                     NLLIQPVVGMTKPGDVDHFTRVRCYEHLLKAYPEQTTALSLLPLAMRMGGPREAVWHA
                     IIRKNYGCTHFIVGRDHAGPGSDSNGKPFYGPYDAQELMKKHEKELDITMVPFKNMVY
                     VEDKAQYFPEDEVEKGTRTLDISGTELRRRLQEGSDIPEWFSFPDVVAELRRTHPPRH
                     KQGFTVFFTGLSGSGKSTVANALMVKLMEMGGRPVTLLDGDLVRKHLSSELGFSKEHR
                     DLNIQRIGYVASEITKNGGIAICAPIAPYTATRKIVREMIEPLGGFIEIHVATPIEVC
                     EARDRKGLYAKARAGILKGFTGIDDPYEAPVNPEMRLDTSDATPDMSAHRIIVKLESL
                     GFIK"
     gene            complement(1995..2969)
                     /locus_tag="VM692_15890"
     CDS             complement(1995..2969)
                     /locus_tag="VM692_15890"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HUQ53710.1"
                     /translation="MADKKILYLFPGQGSQYRGMGSDLVNEFAAAKEIYTRASAVVGY
                     DMTELAFTDPRDELNKTRFTQPALLTHEIACLVSFRTLVGDRVRPLLGAGHSLGEYSA
                     LVLAGALTFENALKLVSRRGALMSELGRGGMVATTLDLQSASALADKYFCGIGAHNMP
                     DQTVIAGESADLDKLCAEMEAAKKRAVRLNTEGAFHTYLMVAAAQQFREVLDATEFAA
                     VTTDVVSNYTGKLHTSSPEAIRSRLFFQLFNPVQWVGCMNTAMDAGFDAAIEFGGGIG
                     KGEGPEGKRPNLETIVKKSLKTREHQAQYLPAINAATIHATAEQLLAA"
     gene            complement(3053..4402)
                     /locus_tag="VM692_15895"
     CDS             complement(3053..4402)
                     /locus_tag="VM692_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ53711.1"
                     /translation="MSINNVLQSGVRPREVWAWAMYDFANSGYTTVVITAVFNAYFVG
                     VVAAGQSWGTFAWTLALSVSYAIIIVTAPVIGAYADAHAVKKKLLAITTSGCVLGTAL
                     LSLVGPGDLALAFALIVLSNVFFGTGENIAAAFLPELARGEGMGKVSAWGWSLGYIGG
                     LITLAICLAYVIWARGQGQTEAQFVPVTMLITAGVFTLASSLTFLVLKERARPLVVPA
                     GGRPLVVPAGGRPPRAEAGAPLRAFARVGQTIRHARQFEDLLRFLGCIVLYQSGVQTV
                     IALAAIYAQEAMGFTTQDTIVLVLLVNITASLGAFVFGQWQDRLGHRKTLTLTLLGWC
                     VMVAIAYLTTERVGFWVAANIAGLCLGASQSAGRALVGYLTPAGRDAEFFGLWGFAVK
                     LASILGPLTYGAVTWLTDNDHRSAMLITGVFFVLGLVVLTSVDPERGRRAAATTAAE"
     gene            complement(4422..4778)
                     /locus_tag="VM692_15900"
     CDS             complement(4422..4778)
                     /locus_tag="VM692_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53712.1"
                     /translation="MEYFDLVRVVHLDGRPHAPATAPHSRAGHSIGHWEGDTLVVDTT
                     HLTPGTFMNNGFNHSDGLHMTERFRVSPDGNTLWLTQVYEDAAVFGGLAARYMAWTRR
                     PGEYVYPYDCDPSYGE"
     gene            complement(4853..>5800)
                     /locus_tag="VM692_15905"
     CDS             complement(4853..>5800)
                     /locus_tag="VM692_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUQ53713.1"
                     /translation="AGADRAQLAPLVSGADILLGATFRGCMDRAFLTLAPNLRLIAKY
                     TIGVDDVDVDAASALGVLVTHCPTEANWGGVAEGAMAMMLAVLKRVRERDRHVKAGGW
                     REPPIYGTYVGKRGDGYAGITIGIVGLGRAGSRVADLLRPWGARVLAADPYVDPSEFV
                     RHGVERTDLDTLLRDSDVVTLHCNLTTETRGLLDRRRLKLMKPTAVLVNTARGAVVDL
                     DALCDALERHALGAAALDVLPDEPASRDARILKLGDRVLLSPHMIAANQGGTLGAAIP
                     WAAEAVLAALRGGVPQRVYNTEAVAKWQARFGGKPLLPA"
BASE COUNT         1001 a         1925 c         1879 g          995 t
ORIGIN      
        1 gaagaccgtc cctggggctc gttggcgcac atccctgtgc gccgacggtc tcgcgagagc
       61 ctctttgcaa ccgcaccgga ctcaagggtt tcgcccgcgc gcgcgcgtgt ctcgcgagcg
      121 cctctccgca ccctcaccgg accaccacga gctaaaatgc gcgtgcgacc tactcgcacg
      181 cgcggcaaac gttacttgat gaagccgagc gactcgagct tcacgatgat gcggtgcgcc
      241 gacatgtccg gcgtagcgtc tgacgtgtcg aggcgcatct ccgggttgac cggagcttcg
      301 tacggatcgt cgatgcccgt gaagcccttg aggatgccgg cacgcgcttt cgcgtagagg
      361 cctttgcgat cgcgcgcctc gcaaacctcg atcggcgtcg caacgtgaat ctcgatgaag
      421 ccgccgagcg gctcgatcat ctcgcgcacg atcttgcgcg tcgccgtata cggcgcgatc
      481 ggcgcgcaaa tcgcgatgcc gccgttcttc gtgatctcgc tcgcgacgta accgatgcgc
      541 tggatgttga ggtcgcggtg ctccttcgag aagccgagct cgctcgagag gtgcttgcgc
      601 acgaggtcgc cgtcgagcag cgtcactggc cgcccgccca tttccatgag cttgaccatc
      661 agcgcatttg cgacggtcga cttaccggag ccggacaggc ccgtgaagaa caccgtgaag
      721 ccttgcttgt ggcgcggcgg gtgcgtgcgc cgcagctccg cgacgacgtc gggaaatgaa
      781 aaccattcgg ggatgtcgga gccttcctgc aaccggcgac gcagctccgt gcccgagatg
      841 tcgagcgtac gcgtgccctt ctcgacttcg tcctcgggga agtactgcgc cttgtcctcg
      901 acgtagacca tgttcttgaa cggcaccatg gtgatgtcga gctctttctc gtgcttcttc
      961 atcagctcct gggcgtcgta cgggccgtag aacggcttgc cgttactgtc actgccggga
     1021 ccggcgtgat cgcggccgac gatgaaatgc gtgcagccgt agttcttgcg gatgatcgcg
     1081 tgccacacgg cctcgcgcgg accgcccatg cgcattgcga gcggcagcag cgatagcgcc
     1141 gtggtctgct cgggatacgc cttcagcagg tgctcgtaac agcgcacgcg cgtgaagtga
     1201 tccacgtcgc ccggcttcgt catgccgacg acgggctgaa tcagcagatt cgcctcgacg
     1261 tccttggccg cgcggaacgt caactcctgg tgagcgcggt gcatggggtt gcgtgtctgg
     1321 aacgcgacga tgcggcgcca gccgagcttc tggaaacgat cgcgaagctc cttaggagta
     1381 tcgcgtaagt gcttgaaatc gtaatggcct ggggcctcga cgccacgcag cttcccgcct
     1441 agatagacgg ggttgctgcg cgacttcagg tagctgacgg ccgggtgctt caggtccgtc
     1501 gtgccaaaaa cggccttcgc ttccacgtcg aaatccgggc ggaagatgct cgatacctgc
     1561 atcgtcgccg ttagcacgcc ctcgccgtcc cgcagcgcga tcgagtcgcc gaccttgagc
     1621 ttgctcgcaa actgttccgt gacgtcgagg ttgatcggca tcggccagag cacgccgctc
     1681 gtcagccgca tctccgtgag cacgcgcttg tagtcggcct cgtccaaaaa accttcgagc
     1741 ggcgagaacg cgccgttcag cagcatctcg atgtcgcaca actggcgcat gttcagatcc
     1801 cacgacgcca agtcgcgcgc gcgtgccttc tcttgcttcg cgcccgcttc gtcgaggtac
     1861 aagttcttca acgccccgcc gtggggatct ttcaagccag ccatttgagt tttgctcccg
     1921 tgtgaaatct ggtgaatcgc ccgcgtgccg aggcgttcaa cctctggcgg cggtgcttgt
     1981 ctcgtgtcgt tgcgctacgc ggccagaagt tgctcggccg tggcgtggat cgtcgccgcg
     2041 ttgatcgcgg gcaggtattg tgcttgatgc tcgcgggttt tcaacgactt cttgacgatg
     2101 gtctcgaggt tcggccgctt gccttcgggc ccttcgcctt tgccgatgcc gccgccgaac
     2161 tcgatcgcgg cgtcgaagcc tgcatccatc gccgtgttca tacagccaac ccactgcacg
     2221 gggttgaaca actggaagaa caaacgcgag cgaatcgctt ccggcgagct ggtgtgcagc
     2281 ttgcccgtgt agttcgagac gacgtccgtc gtgaccgcag cgaactcggt cgcatcgagc
     2341 acctcgcgaa actgctgcgc ggccgcgacc atcagatacg tgtggaacgc gccttcggtg
     2401 ttaagacgca cggcgcgctt cttggccgct tccatctcgg cgcacagctt gtcgaggtcc
     2461 gcagactcgc cggcgatgac cgtttgatcc ggcatgttgt gcgcgccgat gccgcagaag
     2521 tatttgtcgg cgagcgccga agcggactgt agatcgagcg tcgtcgcgac cataccgccg
     2581 cgcccgagct cgctcatcaa cgcgccgcgc cggctcacga gcttcagcgc gttctcgaac
     2641 gtcagggcac cggccagcac gagcgccgaa tactcgccga ggctatgccc cgcgccgagc
     2701 aggggacgaa cccggtcgcc gacgagcgtg cgaaacgata cgaggcaggc gatctcgtgc
     2761 gtcagcagcg cgggctgggt gaaacgggtc ttgttcagct cgtcgcgggg atcggtgaac
     2821 gcgagctccg tcatgtcgta gccgacgaca gcgctcgcgc gcgtgtagat ttccttggca
     2881 gccgcaaact cgttgacgag gtccgagccc atgccccggt actgggaacc ctgaccgggg
     2941 aaaagataaa gaatcttctt gtcggccact ctgcggacta gccttcggag ctcggtgagg
     3001 cgcgtacgat gccccaacgc tgccgttaag ccaagacgaa cctggaatcg cgttattccg
     3061 cagcggtagt tgccgcggcg cgtcgcccgc gctcggggtc gacgctcgtg aggacgacca
     3121 agccgagcac gaagaacacg cccgtgatca gcatggcgct gcgatggtcg ttgtcggtca
     3181 gccaagtgac ggcgccgtag gtcagaggcc ccaagatcga ggccagcttg acggcaaagc
     3241 cccacaagcc gaagaactcc gcgtcacggc ccgcgggcgt caggtagccg acgagcgccc
     3301 ggcctgcgga ttggctcgcg ccgaggcaga gcccggcgat attcgcggcg acccagaagc
     3361 cgactcgctc ggtggtcaga tacgcgatgg cgaccatgac gcaccagccg agcagcgtca
     3421 gcgtcagagt cttgcggtga ccaagccgat cctgccactg cccgaacacg aacgcgccga
     3481 ggctcgcggt aatgttgacg agcagcacga gcacgatcgt gtcttgcgtc gtgaagccca
     3541 tggcttcttg agcgtagatc gcggcgagcg cgatgacggt ttgcacgccc gactgataca
     3601 gcacgatgca acccaagaag cgcagcagat cttcgaactg ccgcgcgtga cggatggtct
     3661 gccccacgcg cgcgaatgcg cgcaacggcg cccccgcttc ggcgcgcggt gggcgccccc
     3721 ctgcggggac taccaagggg cgcccccctg cggggactac caagggtcgc gctcgctcct
     3781 tgagcacgag gaacgtcagc gagctcgcga gcgtgaacac gccggccgtg atcaacatcg
     3841 tgacgggcac gaactgcgct tcggtctgcc cctggcctct ggcccaaatc acgtaagcca
     3901 agcagatggc gagcgtgatc agtccgccaa tgtagccgag cgaccagccc caagccgata
     3961 ccttgcccat gccctcgccg cgcgcgagct ccggcaagaa cgcggctgcg atgttttcgc
     4021 ccgtgccgaa gaacacgttc gacaaaacga tcaacgcgaa cgcgagtgcg agatccccgg
     4081 gccccacgag cgacaacagc gcagtcccga gcacgcagcc cgacgtcgtg atcgcgagca
     4141 gcttcttctt gacggcatgc gcgtcggcgt acgccccaat gaccggcgcc gtgacgatga
     4201 tgatcgcgta gctgaccgac aacgccaacg tccacgcgaa cgtgccccag ctctgtccgg
     4261 cggccacgac gccgacgaaa tacgcgttga acacggccgt gatgacgacg gtcgtgtaac
     4321 ccgagttggc gaagtcgtac atcgcccagg cccagacctc ccgtgggcga acgcccgatt
     4381 gcagaacgtt gttgatgctc atcgagcggg gactttaagc gctactcgcc gtagctggga
     4441 tcgcagtcat acgggtagac gtactcgccg ggacggcgcg tccacgccat gtagcgagcc
     4501 gcgaggccgc cgaacacggc ggcgtcctcg taaacctgcg tcagccacag cgtgttgccg
     4561 tcggggctca cgcggaaacg ctcggtcatg tgcaggccgt cgctgtgatt gaagccgttg
     4621 ttcatgaacg tgcccggcgt caggtgcgtc gtgtcgacga ccagcgtgtc gccctcccaa
     4681 tggccgatcg agtgacccgc gcgcgaatgc ggcgccgtcg ctggcgcatg cggtcggccg
     4741 tcgagatgca cgacgcgcac gagatcgaag tactccatct tgaagacgac gagctcatcg
     4801 ctctgataga cctcttatga gcacctcgat ggcaagcgtc cgcagtatat cgtcacgccg
     4861 gcagcaatgg cttgccgccg aagcgcgctt gccacttcgc gacggcctcg gtgttgtaga
     4921 cgcgctgcgg caccccgccg cgcagcgccg cgagcacggc ctcggcagcc cagggaatcg
     4981 cggcgccgag cgtgccgcct tgattcgcag cgatcatgtg cggtgacagc aacacgcggt
     5041 cgccgagctt gagaatccgc gcatcgcgcg acgccggctc gtcgggcaac acgtcgagcg
     5101 ccgcggcgcc gagcgcgtgc cgctcgagcg cgtcgcacaa ggcgtcgagg tcgacgacgg
     5161 ccccgcgcgc cgtgttgacg agcacggccg tcggcttcat cagcttgagg cggcgccggt
     5221 cgagcaagcc gcgcgtctcg gtcgtgaggt tgcagtgcag cgtcacgacg tcggagtcgc
     5281 gcagcagcgt gtcgagatcc gtccgctcga caccgtggcg cacgaactcg ctggggtcga
     5341 cgtacgggtc ggccgcaagc acgcgcgcgc cccacggtcg cagcagatcg gcgacgcggg
     5401 agccggcgcg gccgagcccg acgataccga tcgtgatgcc ggcgtagccg tcgccgcgtt
     5461 tgccgacgta cgtgccgtag atcggcggct cacgccaacc gccggccttg acgtgacggt
     5521 cgcgctcgcg cacgcgcttc aataccgcga gcatcatcgc catcgcaccc tcggcgacgc
     5581 cgccccagtt cgcttcggtc ggacagtgcg tgacgagcac gccgagcgcg ctcgcggcgt
     5641 cgacgtcgac gtcgtcgacg ccgatcgtat atttcgcgat gagccgcagg ttcggcgcca
     5701 gcgtgaggaa tgcgcggtcc atgcacccgc ggaacgtcgc gccgagcagt atgtcagcgc
     5761 cgctgacgag cggtgcgagc tgcgcgcggt cggcgccggc
//
LOCUS       DATLEE010000725         6628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11074555, whole genome shotgun sequence.
ACCESSION   DATLEE010000725 DATLEE010000000
VERSION     DATLEE010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11074555"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VM692_15910"
     CDS             <1..516
                     /locus_tag="VM692_15910"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HUQ53714.1"
                     /translation="GYALLMRPGSKQLGLHGGNTPWDPAQDYRYRDGEMMNGLMVMSF
                     ALTDAGPDDGGFAVVPGSHKANFPVPKRFISLEETGPWVARVPVQAGDCIIFSEACTH
                     GTWPWRAQHERRSLLYKFCPGHMAWASPYPTPADAPDIDYSEQLQRILEQPYAAPGQD
                     AFGAEYRKTVM"
     gene            complement(526..2679)
                     /locus_tag="VM692_15915"
     CDS             complement(526..2679)
                     /locus_tag="VM692_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HUQ53715.1"
                     /translation="MKIKIETLLGRMSLAEKLGQMTMTSASYAVTGPVIAGDSTESIR
                     NGTLGNLLNLVGAKHVHDMQRIAVEESRLGIPLLIGFDVIHGHRTQFPVPLAEAATFD
                     AGIWELTARESAREMAADGLAMTFAPMLDVSRDPRWGRTVEGPGEDPWLAVQIARAKT
                     RGFQGAELAHAESVAATAKHFCAYGPVAGGRDYAPVDISERTLREVHLPAFAAAVHAG
                     TVAIMPAFTDLAGIPMTAHKALLRDYLRGELGFDGVLVSDYNAIRELIQHGVAADIVD
                     AAVLALKAGVDIDMMAEAYRKGLPVALERGLVTIDEIDECVLRVLRLKERLGLFDDPY
                     RRGATAEAEEAVAARRRLAREVGAKSLVLAKNSADTLPLAKGARRICVVGPLADASRE
                     MRGPWAAAGYEAPNVTALAGLRTALPDAIVEHAEGVAISRTDRSGIAAAVDLVGSGAD
                     LVVLCLGEAAEMSGEAACRVCPELPGEQRALAEAVLARARAGNIPVVAVLFCGRPLMV
                     PWLAEQADALLVAWFPGTEAGNAIADVLTGRVSPSGRTPITWPRAVGQIPLFYAQRNS
                     GRPENSQDHYTSKYLDSPNSPLFAFGHGLTYGRCRHSNLSVSPASATEREQIEVAVDV
                     TNEGMHAAEETVFLFVRDRVASVARPTLELKGVTKVALEPGQTATARFGLPAAELRFL
                     GMDLEPTFEPGEVEIRVGPCADPAQLLSASLMLRA"
     gene            2899..4152
                     /locus_tag="VM692_15920"
     CDS             2899..4152
                     /locus_tag="VM692_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HUQ53716.1"
                     /translation="MLDAVQRQTFRYFWEEAHPTSGLAFDRRTAGTRAADDPWKSPKG
                     DSDPITIGGSGFGAMAIIVATQRGWITRDAALERLDRMLELLLRARCYHGALPHFMNG
                     STGETIAFGRKDDAADLVETSLLLMGLLCVREYFQRDTPQEAAVRSRITTLWNDVEWT
                     WFTRGADEVLYWHWSPYHGWAMDHQIRGWNECLITYVLAASSPRYAIDPGVYHRGFAS
                     GGGFSNRKSWYDIELPLGMSHGGPLFFAHYSFCALDPRGLSDAYADYWAQNVHHVQIN
                     RAHCVANPGKFVGYGESCWGLTASDNPWGYSAHAPDNDNGTISPTAALASMPYAPAEA
                     MTVLRHLLQRHGKRVWRELGFVDAFCESEGWYADTFLAIDQGPIIVMIENHRTGLLWQ
                     LFMAVPEIQAGLRRLGFSSPHLKGQ"
     gene            4149..5498
                     /locus_tag="VM692_15925"
     CDS             4149..5498
                     /locus_tag="VM692_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HUQ53717.1"
                     /translation="MTASLERWLGREYVHAATAMLRSVSARDLAHERPGFGHVVRPAA
                     GSIVASPVGAHWDPDPDYFFHWFRDSAIVIDTVRLLYEAEQLGAEALTHFADFVRFSL
                     SLKALDGRVLVESPHWRSKVEPEFVQYLRTDDELGLVAGDAVYADTRVNPDGTLDISR
                     WTRPQHDGPPLRALAVRRWLRTLRDQPGRASEATLADAERLLRADLALTARQWREPSL
                     DIWEEELGHHYYNLLVSAAALREGAEWLRAAGEEAEGAALHAEARAIRAALDDYWLPH
                     AAHYRSRIMPPGVTSTKLLDIAVILAAVHAGGGEDVHSARDPKMHATLGKLEQLFDGL
                     YPINRDRPRERAPALGRYEGDSYFSGGAYYFATLGGAELCFAAADAHTPGSAERAAWV
                     AHGDAFLETVRVFTPASGDLAEQFDQRTGEQTSAKHLAWSYAAFISCVTARKTALSP"
     gene            5495..>6628
                     /locus_tag="VM692_15930"
     CDS             5495..>6628
                     /locus_tag="VM692_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HUQ53718.1"
                     /translation="MTATKNSGTAADNAETARAGVTYQARMIYRAILASPVGRRVIVL
                     ATVLVVAILITSYGQVVLNDWNQPFYDAITRRDLNEFLYQLGVYFVIVASLLVLDVSQ
                     RWITETFKFRLREGLTRDLLKLWMAPRRAFWLATSGNQLGVNPDQRISEDADKLCNIS
                     ADLSIGLFRASVLLVSFAGVLWTISADFTFRVGGVDYAVPGFMLWAAISYAIVGSLLS
                     YFVGRSLIRRNADRYAREADLRFALVRISEHVDGIALASGEADERRRVEMHIGNVMLA
                     MRRLVRGLTNLTWVTAGFGWITGIAPILISAPLYFSGKTSFGGMMLAAAAFTQAQGSL
                     RWFVDNFGAIADWRATLLRVANFRAALVATETQEDAGSRIEYVD"
BASE COUNT         1060 a         2247 c         2222 g         1099 t
ORIGIN      
        1 ggatacgcgc tgctaatgcg tccgggcagt aagcagctcg gactgcacgg cgggaacacg
       61 ccgtgggatc cggcgcagga ctaccgctac cgcgacggcg agatgatgaa cggactgatg
      121 gtcatgtcgt tcgcgctgac cgatgcgggc cccgacgacg gcgggttcgc cgtcgtgccc
      181 gggagccaca aggccaactt tcccgtgccg aagcgcttca tctcgctcga agagacgggg
      241 ccgtgggtgg cgcgcgttcc agtgcaggcc ggcgactgca tcatcttcag cgaagcgtgc
      301 acgcacggaa cttggccgtg gcgcgcgcag cacgagcggc gcagcttgct gtacaagttt
      361 tgtccgggac acatggcgtg ggcgtcaccg tacccgacgc cggccgacgc acccgacatc
      421 gactactcgg agcagctgca gcggatactc gagcagccgt acgccgcgcc ggggcaggac
      481 gcgttcggcg cggagtaccg caagaccgtg atgtaagcgc agcgcttacg cgcgcagcat
      541 caagctcgcc gacaacagct gcgcgggatc tgcgcagggc ccgacacgaa tctcgacctc
      601 gcccggctcg aacgtaggct cgaggtccat acccaagaag cgcagctcgg ctgcgggcag
      661 accgaagcgc gccgtcgcgg tttggcccgg ctccagcgcg accttcgtca cgcccttcag
      721 ctcgagcgtc gggcgcgcga cgctggcaac gcgatcgcgc acgaacagga acaccgtctc
      781 ttcggccgcg tgcatgcctt cgttcgtcac gtcgacggcg acttcgattt gctcgcgttc
      841 cgtcgcgctc gccgggctga ccgacaggtt cgagtgacgg caacggccgt aggtgagccc
      901 gtggccgaac gcaaacagcg gtgaattcgg cgagtcgaga tacttgctcg tgtagtggtc
      961 ttggctgttt tccggccgcc cggaattgcg ttgcgcgtag aacagcggga tctggccgac
     1021 cgcgcgcggc caagtgatcg gcgttcgtcc gctcggcgac acgcggcccg tcagcacgtc
     1081 ggcgatcgcg ttgccggctt ccgtgcccgg aaaccacgcg acgagcagtg cgtcggcttg
     1141 ctccgcgagc cacggcacca tcagcgggcg gccgcagaac agcaccgcga cgacggggat
     1201 gttgccggcg cgggcgcgcg cgagcacggc ctcggcgagc gcgcgttgct cgcccggcaa
     1261 ttcggggcag acacggcacg cggcttcgcc gctcatctcg gcggcttcgc caaggcagag
     1321 cacgacgaga tccgcgccgc tgccgacgag gtcgacggcg gctgcaattc ccgatcgatc
     1381 ggtgcgcgaa atcgcgacgc cttcggcgtg ctcgactatt gcatccggca acgctgtgcg
     1441 caggcccgcg agcgcggtca cgttcggcgc ttcgtagccg gccgcggccc acgggccgcg
     1501 catttcgcgc gatgcgtccg cgagcggccc aactacgcag atacggcgcg cgcctttggc
     1561 cagcggcaac gtgtcggcgg agttcttcgc gagcacgagc gacttcgcgc cgacctcgcg
     1621 cgccaaacgg cggcgtgcgg caacggcctc ctccgcctcg gcggtcgcgc cgcgccgata
     1681 cggatcgtcg aacagtccga gacgttcctt gagccgcaac acgcgcagca cgcactcgtc
     1741 gatctcgtcg atcgtcacga ggccgcgctc gagcgcgacg ggtaagccct tgcgatacgc
     1801 ctccgccatc atgtcgatgt cgacgccggc cttcagcgca agcacggccg catcgacgat
     1861 atcggccgcg acgccgtgct ggatcagctc gcgaattgcg ttgtaatcgc tgacgagcac
     1921 gccgtcgaaa ccgagctcgc cgcgcaggta gtcgcgcagc agcgctttgt gcgccgtcat
     1981 cgggatgccg gcgagatccg tgaaggccgg catgatcgcc acagtgccgg cgtgcacggc
     2041 cgccgcgaac gccggcagat gcacctcgcg cagtgtgcgc tccgagatgt cgacgggggc
     2101 gtagtcgcgg ccgcccgcca cggggccgta cgcgcagaag tgcttggcgg tcgccgccac
     2161 ggactcggca tgcgcgagct cggcgccctg aaagccgcgc gttttcgcgc gcgcgatctg
     2221 cacggcgagc cagggatctt cgccggggcc ctccacggtt cggccccagc gcgggtcccg
     2281 cgagacatcg agcatcggcg caaaggtcat cgcgagaccg tccgcggcca tttcgcgcgc
     2341 agattcgcgc gccgtgagct cccagatgcc tgcgtcgaac gtcgcggctt ccgccagcgg
     2401 cacggggaac tgcgtgcgat gcccgtggat cacgtcgaag ccgatcaaga gcggaatgcc
     2461 gagccgcgac tcctcgacgg cgattcgctg catgtcgtgg acatgcttcg cgccgacgag
     2521 gttcagcagg ttgcccagcg tgccgttgcg gatcgattcc gtcgaatcgc cggcaatgac
     2581 gggccccgtg accgcgtagg acgcagacgt catggtcatc tggcccagct tctcggcgag
     2641 gctcatacga cccagcagcg tttcgatctt gatcttcatg gcgacaatca tccttgattg
     2701 gcgagagaat atgacgcgcg cctgccggcg agccggcatg agacaggagg tcacttgcgc
     2761 aaatcgaaat tgtcccgagg gagcacgtcc gccgcaacac gccgttcacg ccgcacggcg
     2821 gcgaccgcca aggttgcgcg caagggctcg gccgccgcgc gggatcccgc gaggcttgcg
     2881 gcgctcgagg acgaagcgct gctagacgcc gtccagcggc aaacgttccg ctatttctgg
     2941 gaagaagccc atccgacgag cggtctcgcg ttcgaccggc ggacggccgg cacgcgcgca
     3001 gcggacgacc cttggaagtc cccgaagggg gactccgacc ccatcacgat cggcggctcc
     3061 ggcttcggcg cgatggccat catcgtcgcg acgcagcgcg gctggatcac gcgcgacgcg
     3121 gctctcgagc gcctcgaccg catgctcgag ctgctgctgc gcgcgcgctg ctatcacggc
     3181 gcattgccgc atttcatgaa cggcagcacg ggcgagacca ttgcgttcgg gcgcaaggac
     3241 gacgcggcag acctcgtcga gacgtcgctg ttgctgatgg gcctcttgtg cgtgcgcgag
     3301 tatttccagc gcgacacgcc gcaagaggcc gcggtccgct cgcgaatcac gacgctgtgg
     3361 aacgacgtcg agtggacctg gttcacgcgc ggcgcagacg aggtgctcta ttggcattgg
     3421 agcccgtatc acggctgggc gatggatcac cagattcgcg gctggaacga gtgcctcatc
     3481 acctacgtgt tggcggcttc gtcaccgcgc tacgcgatcg atcccggcgt ttatcaccgc
     3541 gggttcgcaa gcggtggcgg atttagtaat cgcaaatctt ggtacgacat cgagctgccg
     3601 ctcggcatgt cgcacggggg gccgctgttt ttcgcgcatt actcgttctg cgccctcgac
     3661 cctcgcggcc tcagcgacgc ttacgccgac tactgggcac agaacgtgca tcacgtgcag
     3721 atcaatcgcg cccattgcgt tgcgaatccg gggaaattcg tgggctacgg cgaatcttgt
     3781 tggggactaa cggcgagcga caatccgtgg ggctactccg cgcatgcgcc cgacaacgac
     3841 aacggcacga tcagccccac ggccgccctc gcgagcatgc cctatgcccc tgccgaggcg
     3901 atgaccgtgc tgcgccactt gttgcaacgg cacggcaaac gcgtctggcg cgagcttggg
     3961 ttcgtcgacg cgttctgcga aagcgaaggc tggtacgcgg atacgttcct ggccatcgac
     4021 caagggccaa tcatcgtgat gatcgagaac catcggacgg gtctcctgtg gcagctcttc
     4081 atggcagtgc cggaaatcca ggctgggcta cgccggctcg ggttctcgag ccctcacctg
     4141 aagggacagt gacggcaagc ctcgagcgct ggctcggtcg cgagtacgtc cacgccgcga
     4201 ctgcgatgct cagaagcgtc tccgcgcgcg accttgcgca cgagcgcccg ggcttcggtc
     4261 acgtcgtgcg gccggccgcg ggctcgatcg tagcgtcgcc cgtgggcgcg cattgggacc
     4321 ccgaccccga ctatttcttt cactggttcc gcgactcggc gatcgtcatt gacacagtgc
     4381 gtctgctcta cgaagcagaa cagctcggcg cggaggcgct cacgcacttc gcggacttcg
     4441 tgcgctttag cttgtcgctg aaggctctcg acggccgcgt gctcgtcgag tcgccgcact
     4501 ggcggtcgaa ggtcgaaccc gagttcgtcc agtacctgcg taccgacgac gagctcggcc
     4561 tcgttgcggg cgacgccgtt tacgccgaca cgcgcgtgaa tcccgacggc acgctcgata
     4621 tctcgcgctg gacacgcccg cagcacgacg gcccgccgtt gcgagcactc gcggtgcgcc
     4681 gctggctgcg aacgttgcgc gaccaaccag gccgagcttc cgaggccacg ctcgcggacg
     4741 ccgagcggct gctgcgcgcc gacctcgcgt tgacggctcg gcagtggcgt gagccgtcgc
     4801 tggacatctg ggaagaggag ctcggccacc actactacaa cttgctcgtg tcggcagcgg
     4861 cgttgcgcga aggcgccgag tggttgcgcg ctgccggcga ggaggcagag ggcgccgcgc
     4921 tgcacgcgga agcacgcgcg attcgcgcag cgctcgacga ctattggttg ccgcacgcgg
     4981 cgcattaccg ctcgcgcatc atgccgcccg gtgtgacgtc gacgaagctg ctcgacatcg
     5041 cggtgattct cgcggccgtg cacgccggcg gcggcgagga cgtgcattcc gcgcgcgacc
     5101 caaagatgca cgcgacgctc ggcaagctcg agcaactctt cgacgggctg tacccaatca
     5161 atcgcgatcg gccgcgcgag cgcgcgcctg cgctcggccg ttacgaaggc gacagctatt
     5221 tttccggcgg cgcctattac ttcgcgacgc tcggcggcgc ggagctctgt ttcgcggccg
     5281 ccgacgctca cacgcccggc agcgccgagc gtgccgcgtg ggtggcccac ggcgatgcgt
     5341 ttctcgagac cgtgcgcgtc ttcaccccgg caagcggcga tctcgccgag cagttcgacc
     5401 aacgcacggg cgagcagacg tccgcgaagc acctcgcctg gagctacgcg gctttcattt
     5461 cgtgcgtgac ggcgcgcaag acggcgttgt cgccatgacg gcgaccaaga acagcggcac
     5521 cgccgcggac aacgccgaga ccgcgcgggc cggcgtcacg taccaggcgc gcatgatcta
     5581 ccgcgcgatc ctcgcgtcgc cggtcggccg gcgcgtcatc gtgctcgcga cagtgctcgt
     5641 cgtggcgatt ctcatcacga gctacgggca ggtcgttctc aacgactgga atcagccgtt
     5701 ctacgacgcc atcacgcgcc gcgacttgaa cgagttcctg taccagctcg gcgtctattt
     5761 cgtgatcgtc gcgagcttgc tggttctcga cgtctcgcag cgctggatca cggagacctt
     5821 caagtttcgg ttgcgcgaag gcttgacgcg cgacttgctg aagctttgga tggcgccgcg
     5881 ccgcgctttc tggctcgcca caagcggcaa tcagctgggc gtgaacccgg accagcgcat
     5941 cagcgaagat gcggacaagc tgtgcaacat ttcggccgac ctgtcgatcg ggctctttcg
     6001 cgcgagcgtg ctgttggtga gtttcgcggg cgtgctctgg acgatttccg ccgacttcac
     6061 gttccgtgtc ggcggcgtcg actacgctgt gccgggtttc atgttgtggg ccgctatttc
     6121 ctatgcgatc gtcggctcgc tgctatcgta ctttgtgggc cgcagcctga ttcgccgcaa
     6181 cgccgaccgc tacgcgcgcg aagccgacct gcgcttcgcg ctcgtgcgca tcagcgagca
     6241 cgtcgacggc atcgcgctcg cgtccggcga ggccgacgag cgccgccgcg tcgagatgca
     6301 catcggcaac gtcatgctcg cgatgcgccg gctcgtccgt ggcctgacga atctcacgtg
     6361 ggtcacggcc gggtttggct ggatcacggg catcgcgccg atcttgatct cggcgccgct
     6421 gtacttcagc ggcaagactt cgttcggcgg catgatgctc gccgccgccg cgttcactca
     6481 ggcgcaagga tcgctgcgct ggttcgtcga caatttcggc gcgatcgcgg actggcgcgc
     6541 cacgctactg cgcgtcgcga acttcagggc cgccctggtc gccaccgaaa cgcaggagga
     6601 cgcgggcagc cgaatcgagt acgtcgac
//
LOCUS       DATLEE010000726         5150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7352994, whole genome shotgun sequence.
ACCESSION   DATLEE010000726 DATLEE010000000
VERSION     DATLEE010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7352994"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2266
                     /locus_tag="VM692_15935"
     CDS             <1..2266
                     /locus_tag="VM692_15935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HUQ53719.1"
                     /translation="PAPAPAITPSTTTRPAEASRPAANAGAADPAVAAGVEESRRLLE
                     VRNSQLQNVQQAAAATQAAVPPPAANQSAQPGVEVESEQLFADETEPAPAPVAEQPAP
                     APAPAPVVQTPVSSEPSLVSQAIDWLKSPFLWIGAGVTALLLTALWFVRRRRQETEDV
                     TGRWEALESETDDEDTRVATERMRRQLAEETIVVEEQHAQSVRPEPEATRRAPAPRPQ
                     PARSGVQADETLSSQTVINLDQADAVAEADFHIAYGLYDQAAELVQKALEAAPDRRDL
                     KLKLLEVYFMWGNKDAFLKAAQALRADVGQADDADWNKVVIMGRQICPDERLFTEATT
                     GPGRVDVDLEAGDSPLDLAFDDAAATADVSAAASSGLDFDLETSDQRPAPKATAKSAP
                     RDFGGDALDIGARTAAGLEAALFEDLSKDDEGGRTMPDVAADSLAVTQESPTVERPGS
                     SDWLTIESPTAQYAADAPTVETPTIESPAAKSAQTIASKTVSIPRRSESPTVEQPALG
                     GGMGEFTAEIDLDDLGLDVKDIEGLPTDLGDLPMASSHETDTREQPALRDDDSLLSAT
                     GVTKVLHGEDDNDADSGQTSVLADQDATLMAPGFDEGTSTLTGTEVLEGRHEDDESGS
                     TSLVRSLRGKDKGGVDLDLADLTAALHGADTVEQPRSSSFSRDVFGGGDTPLDLDIGV
                     EVASGDDDPTGTEEVGLDPQTMTEVGTKLDLARAYIDMGDPEGARSILEEVLDEGDSN
                     QRREAQGLIDVLSA"
     gene            2318..3103
                     /gene="truA"
                     /locus_tag="VM692_15940"
     CDS             2318..3103
                     /gene="truA"
                     /locus_tag="VM692_15940"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005768766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HUQ53720.1"
                     /translation="MPRIALGLEYDGTDFVGWQIQRTGRSVEGTLAAAVSFVAGEPVN
                     VHGAGRTDAGVHALQQVAHFDASVSRSPRQWLLGINSNLPPDVAIRWVQEMPADFDAR
                     RSAVSRRYRYTVLQQQPRPALLRRRVWWLRQPLDYAAMTAAASAWLGEHDFSAFRGAG
                     CQAKSPMRRLMGVAIAKRSRGEGALVTFEFTANAFLQHMVRNLVGTLVAVGSGELAAA
                     DAGTILERRDRRQAGVTAPPEGLALVEVLYAPHFGLPRCTDEP"
     gene            3159..3917
                     /locus_tag="VM692_15945"
     CDS             3159..3917
                     /locus_tag="VM692_15945"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HUQ53721.1"
                     /translation="MSWFDKLMPSRIRTEKRKHSVPEGLWVKCSGCSAQLYRAELNRN
                     LHVCPKCGHHLRVGARERLEMFLDAESGYELAANLEPLDPLRFKDTKRYRERLNQAQR
                     ETDERDALVVMAGTLKSMPIVAAAFEFRFISGSMGSVVGERFVRAVAYCIQHQRPLIC
                     FAASGGARMQEALYSLLQMAKTSSALAQLAAAKLPYISVMTDPTTGETLPEGFQRAEF
                     LLEKGAIDMILDRRHARDQIASLLAKLSAKPVPS"
     gene            3937..>5150
                     /locus_tag="VM692_15950"
     CDS             3937..>5150
                     /locus_tag="VM692_15950"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HUQ53722.1"
                     /translation="MTTPPQSGLTLDRWLAWFETVHPKRIDLRLDRFVAVLDKLGLRA
                     PPYKLVTVAGTNGKGSCVAMLESIYWHAGYDVGTFTSPHLWRFNERIRVNGVDAADAE
                     LVAVFEEITEALGSTTLSYFEASFVAALALFQRHDLDVAILEVGMGGRLDATNAVDTD
                     CALIVSIDLDHREWLGETREAIGREKAGIVRAGKPVVIGDRAVPSSVLEHAAETGATP
                     YLIGRDFDFRRDGLRWRRARDAESAPSFPLPPFGGDEQFANAASCAAVVERLSSSLPV
                     PDAALAGGIESAVLRGRLERHEVDGVEWVFDVGHNPAAAEMLAASLRRLPLAHRTWVV
                     FAAMRDKDLAGVVEPLVPGAAGWFVGQASADRGATGEELGTLLDSLGAARVVVAADIA
                     AACAAAGKAATAG"
BASE COUNT          831 a         1736 c         1791 g          792 t
ORIGIN      
        1 cccggcgccc gcgcctgcga tcacaccgtc gacgacgacg cggcccgccg aagcgtcgcg
       61 cccggcagcg aacgccggtg cggccgaccc cgccgtcgcc gcgggtgtcg aggagagccg
      121 ccggctgctc gaggtcagga actcgcagct gcaaaacgtc cagcaagccg ccgctgccac
      181 gcaggccgcc gtgccgccgc ctgccgcgaa ccaatctgcg caacccggcg tcgaggtcga
      241 gtccgagcaa ttgttcgccg acgaaaccga accggcgccc gcacccgttg cagaacagcc
      301 tgcgccggcc ccggctcccg ctccggtggt gcagacgccg gtgtcgtcgg agccgtcgct
      361 cgtttcgcag gcgattgatt ggctcaaatc gccgttcctt tggatcggcg cgggcgtaac
      421 cgcattgcta ctgacggccc tgtggttcgt gcgccgtcgc cgccaggaga cggaagacgt
      481 cacaggtcgt tgggaagcgc tcgaatcgga gaccgacgac gaggacacgc gcgtcgcgac
      541 cgagcgcatg cgacggcaac tcgcggagga gacgatcgtc gtcgaggagc agcacgcgca
      601 gagcgtgcgg cccgagcccg aggcgacgcg tcgcgcgccc gctccgcggc cgcagccggc
      661 acgttccggc gtgcaggccg acgaaacgct gtcgagccaa acggtcatca atctcgacca
      721 ggccgatgcc gtggccgagg cggatttcca cattgcgtat ggcctctacg accaagcggc
      781 cgagctcgtg cagaaagcgc tcgaagccgc tcccgatcgc cgcgacctca agctgaagct
      841 gctcgaggtc tatttcatgt ggggcaacaa ggacgccttt ctcaaagccg cccaagcgct
      901 gcgcgccgac gtcggccaag cagacgatgc cgactggaac aaggtcgtca tcatgggccg
      961 gcagatctgc cccgacgagc gattgttcac ggaagcgacg accggcccgg gccgagtcga
     1021 cgtcgatctc gaagccggcg actcgccgct cgacctcgcg ttcgacgacg ctgcggcgac
     1081 ggcggacgtg tcggctgccg caagctcggg tctcgatttc gatctcgaaa cctccgacca
     1141 gcgtccggcg ccgaaggcga ccgcaaagtc cgcgccgcgc gacttcggcg gcgatgcgct
     1201 cgacatcgga gcgcgcacgg ccgcaggtct cgaggccgcg ctgttcgagg atctgtccaa
     1261 ggacgatgag ggcggacgca cgatgcccga cgtcgctgcc gacagcctcg ccgtcacgca
     1321 agagtcgccg acagtcgagc ggcccggcag cagcgattgg ctgacgatcg agagcccgac
     1381 ggcgcagtac gccgccgacg cgccgacggt cgagacaccg acgatcgaaa gccccgctgc
     1441 gaagtcggcg cagacgatcg cgtcgaagac cgtctcgatc ccgcggcgct ccgagtcgcc
     1501 gacggtcgag cagcccgcgc tgggcggcgg catgggcgag ttcaccgcgg agatcgatct
     1561 cgacgatctc gggctcgacg tcaaagacat cgaaggtctc ccgaccgacc tcggcgattt
     1621 gccgatggcg tcgtcgcacg agacggacac gcgcgagcag ccggcgctgc gcgacgacga
     1681 ctcgctgttg tcggcgacgg gggtgacgaa ggttctgcac ggcgaagacg acaacgatgc
     1741 ggacagcggc caaacgtcgg tgctcgcgga tcaagacgcg acgctgatgg cgccaggctt
     1801 cgacgaaggc acgagcacgc tgacgggcac cgaagtgctc gaaggccggc acgaggacga
     1861 tgagtccggc agtacgtcgc tcgtcagatc gctgcgcggc aaggacaagg gcggcgtcga
     1921 tctcgatctc gccgatctca ctgcggcttt gcatggcgcc gatacagtag agcagccgcg
     1981 ctcgtcgtcg ttctcgcgtg acgtcttcgg cggcggcgac acgccgctcg atctcgacat
     2041 cggcgtcgag gtcgcgagtg gcgacgacga cccgaccggc actgaggaag tgggtctcga
     2101 cccgcagacg atgaccgagg tcggcacgaa gctcgacctg gctcgcgcct acatcgacat
     2161 gggcgacccc gaaggtgctc gcagcatcct cgaggaagtt ctcgacgagg gcgactcgaa
     2221 ccagcgccgc gaggcgcagg gcttgatcga cgtcttgagc gcctaggcgt ttcggtactg
     2281 ttcgcgcacg gccgcgcttc ttgaggccgc cgcaggtatg ccgcgaatcg cgcttgggct
     2341 cgagtacgac ggcaccgact tcgtcggctg gcagattcag cggaccgggc gcagcgtcga
     2401 aggaacgctg gcggccgccg tgagcttcgt cgccggcgag cccgtaaacg tgcacggtgc
     2461 cggccgcacc gacgccggcg tgcacgcact gcaacaagtc gcgcacttcg acgcgagcgt
     2521 cagccgctcg ccgcgccagt ggctgctcgg tatcaactcg aatctgccgc cggacgtcgc
     2581 gatccgttgg gtgcaggaaa tgcccgcgga cttcgacgcg cggcgctcgg ccgtcagccg
     2641 gcgctaccgc tacacggtgc tgcagcaaca accgcggcct gcgttgctgc ggcggcgtgt
     2701 ttggtggctg cggcagccgc tcgattacgc ggcgatgacg gcggccgcgt cggcgtggct
     2761 cggcgagcac gatttctcgg cgtttcgcgg cgcgggctgc caggcgaagt cgccgatgag
     2821 gcgcctcatg ggcgtggcga tcgcgaagcg gtcgcgcggc gagggggcgc tggtaacgtt
     2881 cgagttcact gcgaacgcgt tcctgcagca catggttcgc aacctcgtcg gcacgttggt
     2941 tgccgtcggc agcggcgagc ttgcggcggc cgacgccggc acgattctcg agcgtcgcga
     3001 ccggcggcaa gcaggcgtca cggcgccgcc cgaggggcta gctctcgtcg aggtgctgta
     3061 cgcgccgcat tttggcttgc cgcgctgcac ggacgagccg tgatggcaac gcggtgcttt
     3121 cggctatcat gcggtcagtc ttccactcaa gcgcgcgtat gagctggttc gataaattga
     3181 tgccgtcacg gatccgcacc gaaaagcgca agcactcggt gcccgagggt ctgtgggtca
     3241 agtgctcggg ctgctccgcg cagctctatc gcgccgagct caatcgcaac ctgcatgtgt
     3301 gcccgaagtg cgggcaccac ctgcgagtcg gcgctcgcga gcgcctcgag atgttcttgg
     3361 atgccgagtc gggctacgag ctcgcggcga acctcgagcc gctcgacccg ctgcgcttca
     3421 aggacacgaa acgctatcgc gagcgtttga accaagcgca gcgtgaaacc gacgagcgcg
     3481 acgcgctcgt cgtgatggcc ggcacgctga agtccatgcc gatcgtcgcg gcggcgttcg
     3541 agttccgctt catctcgggc tcgatgggct cggtcgtcgg cgaacgcttc gtgcgtgccg
     3601 tcgcgtattg tatccagcat caacgcccgc tcatctgctt cgcggcgagc ggcggtgccc
     3661 gcatgcagga agcgctttac tcgttactgc agatggcgaa gacgagctcg gcgttggcgc
     3721 agctcgccgc cgccaagctg ccgtatatct ccgtgatgac ggacccgacg acgggcgaga
     3781 cgcttcccga agggttccag cgcgccgaat tcctgctcga gaagggcgcg atcgacatga
     3841 tcctcgatcg gcggcacgct cgcgatcaga tcgcgtcgct gctcgcgaag ctaagcgcca
     3901 agcccgtgcc gtcctgagct cggaagctcg ctgcgcgtga ccactccgcc gcagagcggc
     3961 ttgacgctcg atcgctggct cgcttggttc gagacggtac atccgaagcg gatcgacctg
     4021 cggctcgacc gtttcgtcgc cgtgctcgac aagctcgggc tgcgagctcc gccgtacaag
     4081 ctcgtcaccg tcgccggcac gaacggcaaa ggctcgtgcg tcgcgatgct cgaaagtatc
     4141 tattggcacg ccggttacga cgtcggcacg ttcacgtcgc cgcacctctg gaggttcaac
     4201 gagcgcatcc gcgtgaacgg cgtcgacgcc gcggacgcgg agctcgtggc ggtcttcgag
     4261 gaaatcaccg aggccttggg ctcgaccacg ctgtcgtact tcgaggcgtc tttcgtggcg
     4321 gccctggcgc tgttccaacg tcacgacctc gacgtcgcga ttctcgaggt cggcatgggc
     4381 ggccgtctcg acgcgacgaa tgcagtcgac acggactgcg cgctgatcgt cagcatcgac
     4441 ctcgaccatc gcgaatggct cggggagacg cgcgaggcca tcgggcgcga gaaggccggc
     4501 atcgtgcgcg ccggcaagcc cgtcgtcatc ggcgaccgag ccgtcccgag cagcgttctc
     4561 gagcacgcgg cggagacggg cgctacacct tatttgattg gccgagattt cgattttcgg
     4621 cgcgacggcc tgcgctggcg cagggcgcgc gacgcggaat cggcgccgtc attcccgttg
     4681 ccgcctttcg gcggcgacga gcagttcgca aacgccgctt cgtgcgcggc cgtcgtcgag
     4741 cggctgtcga gctcgttacc cgtgccggac gcggcgctcg cgggcggcat cgaatcggcg
     4801 gtactccgcg ggcggctcga gcggcatgaa gtcgacggcg tcgagtgggt tttcgacgtg
     4861 ggccacaacc cggccgcggc cgagatgctc gcggcgtcgt tgcgacgttt gccgctcgcg
     4921 caccgcacgt gggtcgtgtt cgccgcgatg cgcgacaagg atctcgcagg cgtcgtcgaa
     4981 ccgctcgtgc cgggtgcagc cgggtggttc gtcgggcagg caagcgccga ccgcggcgcg
     5041 acgggcgagg agctcggcac tctgctcgac agcctcggcg ccgcgcgcgt cgtcgtcgcc
     5101 gcggacatcg cggcggcgtg cgctgccgca ggtaaggcgg ccacggccgg
//
LOCUS       DATLEE010000727         4118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10199108, whole genome shotgun sequence.
ACCESSION   DATLEE010000727 DATLEE010000000
VERSION     DATLEE010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10199108"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1646
                     /locus_tag="VM692_15955"
     CDS             <1..1646
                     /locus_tag="VM692_15955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUQ53723.1"
                     /translation="QRAKSTQRPATSSVTTDFAWRVLILLNLFRLSVGALLLAVFYLV
                     DEPRIVGETDETLAWRALIGLLVAGCAFLVLLQRRAPSTRAQTYLQFATDLFAVALLI
                     HASGGISSGLGGVLIVSVGTLALLLPTERAFLLAAVSTLALLGQQTYAQLQGITTGAQ
                     FAPVGILGAVIFVITAVVQLLRARLVETEALAEQRGLDLRNLVELNEYIIQHLRESIV
                     VVDGENQIRLINESAIKHLGRGGSPGELNLGVISSELTSQLRRWRSGANVDRTQMAFT
                     SPDGSNIQPHFAPLSSGRTGGVVIFLEDTSLIAERVQQAKLAALGRLSASIAHEIRNP
                     LGAMSHAGQLLAESPSMGKDEHRLTDIIRVNARRVSQIVESVLALSRREAAHPESLQL
                     IPWVEDFTREFVQTLELYEGAVSLVGNSPDIEVRMDPTHLHQILWNLCDNAVKYASVA
                     AGAIAVEISSGVLEPSGRPYLEVADRGPGIKPDKVEQIFEPFYTSQPGGTGLGLYISR
                     ELAERNGAALRYQPRPGGGSAFRIVFADPRRWQSIEEHA"
     gene            1643..2980
                     /locus_tag="VM692_15960"
     CDS             1643..2980
                     /locus_tag="VM692_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUQ53724.1"
                     /translation="MKPIALVVDDEPHICELLSITLERMDIATRTCLDVTSAKQALGE
                     QAFDLCLTDMRLPDGDGLELIEWIQREVPGTPVAVITAHGSVEAAVRALKLGAFDFVS
                     KPLDLNDLRKLVTAALKLKGAAVPRNSATDGLVGQSPPLERVRAMVAKVARSQAPVHI
                     SGESGTGKELVARMIHETGPRREGPFVPINCGAIPTELMESEFFGHKKGSFTGAVSDK
                     IGLVQTAEGGTLFLDEVADLPLHMQVKLLRVIQEKTIRPVGQANEIAVDVRILSATHK
                     NLGELVAEGSFREDLFYRINVIGLQVPPLRDRGEDVLMLAHHALTRLAGEQGVRTPHL
                     TVAAQEALLDYRFPGNVRELENILERALTLCSGDEIDVADLQLREDNDDESAQRTGND
                     RGGAPLGTQLEEIERDAIVAALEQTRYNKTAAAKLLGLTFRALRYRIKKLGIE"
     gene            3264..3767
                     /locus_tag="VM692_15965"
     CDS             3264..3767
                     /locus_tag="VM692_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HUQ53725.1"
                     /translation="MTKLQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRAQVSEGM
                     NLAAAAKAAVAETFLNRGAAPANRTAAGMSANAGDTRGKYVTGIEVTNGVIQVTYGAE
                     ANTQIAAATLELVPYVSADNSVVWRCGAAPVPTGVAGLMPLAAAGGGTLATNTSNNKY
                     LPAACRP"
     gene            3852..>4118
                     /locus_tag="VM692_15970"
     CDS             3852..>4118
                     /locus_tag="VM692_15970"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUQ53726.1"
                     /translation="MPRRLPSHPRQAVAGFTLPELIGALAVTGIVAAVAVSAYRTYLV
                     RSQIAYSVELSSPIRGRVAAAFRSTGLPLSSVEQPGAGTDSAGGL"
BASE COUNT          721 a         1354 c         1337 g          706 t
ORIGIN      
        1 cgcaacgcgc caaatccacg cagcgccctg caaccagttc ggtgacgacc gacttcgcct
       61 ggcgagtcct gatcctgctg aatttgtttc gcctgtccgt cggcgccttg ttgctggccg
      121 tgttctacct cgtcgacgag ccgcgcatcg tgggcgagac cgacgagacg ctggcatggc
      181 gcgcgctgat cgggttgctc gtcgcgggct gtgcgttcct cgtgctcttg cagcgccgag
      241 caccgagcac gcgcgcgcag acgtatctgc agttcgcgac cgacctcttc gccgttgcgc
      301 tgctgatcca tgccagcggt ggtatctcga gcggtctcgg cggcgtcctc atagtatcgg
      361 tcggcacatt ggccttgctg ttgccgacgg agcgcgcatt tctgctggcg gccgtctcta
      421 cgctggcgtt gctcggccag caaacgtatg cgcagctgca gggcattacg acgggcgcgc
      481 agttcgcgcc cgtcggaatc ctcggcgccg tcatcttcgt gatcacggcc gtcgtgcagt
      541 tactgcgcgc gcggctcgtc gaaacggaag ccctcgccga gcagcgcggc ctcgatctac
      601 gcaatctcgt cgagctcaac gagtacatca tccagcactt gcgcgagagc atcgtcgtcg
      661 tcgacggcga gaaccaaatt cgcttgatca acgagtctgc gatcaagcac ctcggccgcg
      721 gcggtagccc gggcgagctg aaccttggcg tgatctcgtc cgagctcacg agccagttgc
      781 gccggtggcg cagcggcgcc aacgtcgatc gcacgcagat ggcttttacg tcgcccgacg
      841 gcagcaacat tcagccgcac ttcgctccgc tcagcagcgg ccgcaccggc ggcgtcgtga
      901 tcttcctcga agacacgtcg ctgatcgccg agcgggtgca gcaggccaag ctcgccgcgc
      961 tcggccgcct gagcgcgagc atcgcgcacg agatccgcaa tccgctcggc gctatgagcc
     1021 acgcgggaca gctgctggcg gagtcgccga gcatgggcaa ggacgagcat cggctgaccg
     1081 acattattcg cgtcaacgcg cgccgcgtca gtcagatcgt cgagagcgtg ctggcgctgt
     1141 cgcgacgcga agccgcgcat cccgagagct tgcagctgat cccgtgggtc gaggacttca
     1201 cgcgcgaatt cgtgcagacg ctcgagctct acgagggcgc cgtgagcctc gtggggaatt
     1261 cgccggatat cgaagtgcgc atggatccca cgcacctgca tcagatcttg tggaatctct
     1321 gcgacaacgc agtgaagtat gcgagcgtcg cggcgggcgc gatcgccgtc gagatatcga
     1381 gcggcgttct cgagccttcc ggccgccctt atctcgaggt cgccgatcgc gggccgggga
     1441 tcaagcctga caaagtggag cagatcttcg agccgtttta tacgagccaa ccgggcggca
     1501 ccgggctggg tctctatatt tctcgcgagc tcgcagagcg caacggggcc gcgctacgct
     1561 accaaccccg gcccggcggc ggcagcgcgt ttcgcatcgt gttcgccgat ccgcgacgtt
     1621 ggcaatcgat agaagaacac gcatgaaacc catcgccctc gtcgtcgacg acgaacccca
     1681 catctgcgag ttgttgtcga tcacgctcga gcgcatggac atcgcgacgc gcacgtgcct
     1741 cgacgtcacg agcgcgaagc aggcgctcgg cgagcaagcc ttcgatctct gcctgaccga
     1801 catgcggttg cccgacggcg acgggctcga gctgatcgag tggatacagc gcgaagttcc
     1861 gggcacgccc gtcgccgtga tcacggcgca tggcagcgtc gaggcggccg tacgcgcctt
     1921 gaagctcggt gcgttcgact tcgtctcaaa gccgctcgat ctcaacgatc tgcgcaagct
     1981 cgtcaccgca gccctgaagc tcaaaggcgc ggccgtgccg cgcaactcgg cgaccgacgg
     2041 cctcgtcggc caatcgccgc cgctggaacg cgtgcgcgcg atggtcgcca aggtcgcgcg
     2101 cagccaggcg cccgtgcaca tctccggcga gtccggcacc ggcaaggagc tggtcgcgcg
     2161 catgatccac gagaccggcc ccaggcgcga agggccattc gtaccgatca actgcggcgc
     2221 gattccgacc gagctcatgg aaagcgagtt cttcggccac aagaagggaa gcttcactgg
     2281 agcggtcagc gacaagatcg ggctcgtgca aacggcggag ggcggcacgt tgttcctcga
     2341 cgaagtcgcc gacctgccgc tgcacatgca ggtcaagttg ctgcgcgtca tccaggaaaa
     2401 gacgattcgt ccggtcggtc aggcgaacga gatcgccgtc gacgtgcgca tcttgagcgc
     2461 gacgcacaag aacctcggcg agctcgtcgc cgaaggtagc ttccgcgagg acctcttcta
     2521 ccgtatcaac gtcatcggcc tgcaagtgcc gccgctgcgc gatcgcggcg aggacgtgct
     2581 gatgctcgcg caccacgcgc tgacgcggct cgccggcgag cagggcgtgc gcacgccgca
     2641 tctgaccgtc gcggcgcagg aggcgctgct cgactatcgt tttcccggca acgtgcgcga
     2701 gctcgagaac atactcgagc gcgcgttgac gctgtgctcg ggcgacgaga tcgacgtagc
     2761 ggatctgcag ctgcgcgagg acaacgacga cgagtcggcg cagcgaacgg gcaacgatcg
     2821 cggcggcgcg ccgctcggca cgcagctcga ggagatcgag cgcgacgcga tcgtcgcggc
     2881 gctcgagcag acgcgttaca acaagacggc cgcggcgaag ttgctgggcc tgacgtttcg
     2941 tgcgctgcgc tatcgcatca agaagctcgg catcgagtag cgtcgcgctg tcataaagcg
     3001 tgtcgcgcgc tgccgaaaac cgctgacaat tttggtcagt ttgtgcccgt tattcgtctg
     3061 tcgccgacaa gcgtcagtgc gcaatatgtt gaaccttggt cggccgcggc ccgcgggcac
     3121 cgccacgagc cttgctccac ttcgaagttg acatagcgca gagcgcttgg cacaccgatt
     3181 gcaaagcccc cgtcagacgc gtggacgtca ttgcttcgaa taaccacaaa ctttgttttg
     3241 aaaccgctag aaggagcttg gaagtgacga aactgcaaaa gggcttcacc ctcatcgagt
     3301 tgatgatcgt ggtggccatc atcggcatcc tggccgcgat cgcgattcct gcctatcagg
     3361 actacacgat ccgcgctcag gtctcggaag gcatgaactt ggcggcggcc gcgaaagctg
     3421 cggtggccga gacgttcttg aaccgcggcg ctgctccggc taaccgcacg gcggccggta
     3481 tgtcggcgaa cgccggcgac acgcgcggca agtacgtcac gggcatcgag gtgacgaacg
     3541 gcgtgatcca agtcacgtac ggcgcggaag cgaacacgca gatcgctgcg gcgacgctcg
     3601 agctcgtccc ctacgtcagc gccgacaact cggtcgtgtg gcgttgcggc gcggcccctg
     3661 tgccgaccgg cgtcgccggc ctgatgccgc tcgctgcggc gggtggtggt acgttggcga
     3721 cgaacaccag caacaacaag tacctgccgg ccgcctgccg tccgtaagct agtagcgacc
     3781 acggccacgg cgtgtggctg atcgcaaacg aaacgcaagg cccctcgcgc aagcgagggg
     3841 cctttgcttt agtgccacgt agactcccct cacatccgcg ccaagccgtc gcgggcttca
     3901 cgctgcctga gctgattggc gccctcgcag ttacgggtat cgtcgcggcc gtcgcggttt
     3961 ctgcgtatcg cacctatctg gttcgctcgc agatcgccta ttcagtcgag ctgtcgagcc
     4021 cgatcagggg ccgcgtggcg gccgcgtttc gcagcaccgg cttgccgctc tcgagcgtgg
     4081 agcagccagg cgcgggaacg gactccgcgg gcggactt
//
LOCUS       DATLEE010000728         4356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6468551, whole genome shotgun sequence.
ACCESSION   DATLEE010000728 DATLEE010000000
VERSION     DATLEE010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6468551"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /gene="aroA"
                     /locus_tag="VM692_15975"
     CDS             <1..1097
                     /gene="aroA"
                     /locus_tag="VM692_15975"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HUQ53727.1"
                     /translation="QRGLRAPRGVLDLGNSGTAIRLLMGLLAGQPFDSELTGDASLRQ
                     RPMERVAAPLRLMGARITTSGGGRPPVAIGGGARLRGCDYELPMASAQVKSALLLAAL
                     NAEGTTTVRSPGPSRDHTERMLESMGVRLGLAAAGAGHVVTLTGPARLRGFEIRVPAD
                     FSSAAFFLVAGCLGARDGLLIENVGVNPTRTGLLTILREMGATIELRNQRSVGAEPVA
                     DLWVTQSELKGIAVPPELVPLAIDEFPILFIAAAAAAGETVVGGAEELRKKETDRIAV
                     MARGLEAVGVKVEERPDGARIIGRGALRGGSVDSRGDHRIAMSFAVASLKASSAIEIL
                     NTAEVATSFPNFLDVAAAVGLRVSAAEDSA"
     gene            1094..1801
                     /gene="cmk"
                     /locus_tag="VM692_15980"
     CDS             1094..1801
                     /gene="cmk"
                     /locus_tag="VM692_15980"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HUQ53728.1"
                     /translation="MKGTSDSAPVVAIDGPSGSGKGTIGRLLAQRLGWHYLDTGALYR
                     LVALVALKRHIDFGDAPALARAAATLDVRFAPAAAGERVHLDGVEVGGELRTERTGDA
                     ASKVAVVPEVRAALLQRQRDFALPPGLVADGRDVGTVVFPDALLKVVLTASAEARAMR
                     RHKQLKEKGIDVSLPDLSWDIAQRDARDANRTVAPFKPAPDAQVIDSTSLTPEEVVAH
                     ILSRLEGVGVVAQRAKK"
     gene            1876..3555
                     /gene="rpsA"
                     /locus_tag="VM692_15985"
     CDS             1876..3555
                     /gene="rpsA"
                     /locus_tag="VM692_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HUQ53729.1"
                     /translation="MSESFAELLEESFANQKIKTGAILTGIVVGVNADMVIVNAGLKS
                     EAVIPADQFFNERGELEVKVGDHVEVALDTVEDGFGETRLSREKAKRTRTWTRLEESF
                     QKGEIVTGTISGRVRGGFTVDIDLVRAFLPGSLVDVRPIRDPAALEGSTLEFKVIKLD
                     QKRNNVVVSRRAVVEAEYSAERESLLKNLQEGTVLKGVVKNLTDYGAFVDLGGIDGLL
                     HITDMAWKRVKHPSEVVSVGQELEVKVLKFDRERNRVSLGLKQLGADPWADLARRYPP
                     NTRLFGKVTNIADYGCFAEIEEGVEGLVHVSEMDWTNKNVNPHKVVQIGDEVEVMVLD
                     IDEERRRISLGLKQCKPNPWVEFGENYKKGDKVAGQIKSITDFGVFIGLPGGIDGLVH
                     LSDLSWDVAGEEAVRNYKKGEELETTVLAIDPERERISLGVKQLDKDPLSLFLGDHPK
                     GSIVSGVVQELDAKVATIALEGGMIGHLRASEIARDRVEDARTVLKVGETVEARITGV
                     DRKTRIISLSIKAKEVHEEQQALQNYKSETSRTSTGTTLGDLLKEQMNPTE"
     gene            3611..3913
                     /locus_tag="VM692_15990"
     CDS             3611..3913
                     /locus_tag="VM692_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HUQ53730.1"
                     /translation="MTKSELIESIARSQKHLPAKDVELAVKHILELMSEALANGERIE
                     IRGFGSFSLHYRPPRMGRNPKTGEAVALAGKNVPHFKPGKDLRERVNESRHLPVRD"
     gene            3994..4284
                     /locus_tag="VM692_15995"
     CDS             3994..4284
                     /locus_tag="VM692_15995"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HUQ53731.1"
                     /translation="MVTLRRLGFVAVLLVLMLMTGVFAYSNPESIDIDIGLKRFEQVP
                     MAAAFAIVLAVGWLLGLLSAAIALWRSAGEKRRLKQDLKYAEAELRTRQPSA"
BASE COUNT          786 a         1352 c         1471 g          747 t
ORIGIN      
        1 ggcagcgggg attgcgcgcg ccgcgcggcg tgctcgacct cggcaactcg ggaaccgcga
       61 tccgcctgtt gatgggactg ctcgcaggac agccgttcga ttccgagctc acgggcgacg
      121 cgtcgctgcg gcagcggccg atggagcgcg ttgccgcgcc gctgcggctc atgggcgcac
      181 gcatcacgac gtcgggcggc ggccggcctc cggtcgcgat cggcggcggc gcgcggttgc
      241 gcggctgcga ttacgagctg ccgatggcga gcgcgcaagt gaaatcggcg cttttgcttg
      301 cggcgctgaa cgccgaaggt acgacgacgg tccgctcgcc gggcccgagc cgcgaccaca
      361 ccgagcgcat gctcgaaagc atgggcgtgc ggctcgggtt agcggcggcg ggcgcgggcc
      421 atgtcgtgac cttgacgggg cctgcgcggc tgcgcggctt cgagatccgc gtcccggcgg
      481 atttcagctc cgcggccttc ttcctcgtgg cgggctgcct cggcgcgcgc gacggattgc
      541 tgatcgagaa cgtcggcgtg aacccgacgc gcacgggctt gttgacgatc ctgcgcgaga
      601 tgggcgcgac gatcgagctg cgcaatcaac gcagcgtcgg cgcggagccc gtcgcggatc
      661 tttgggtcac gcaaagcgag ctcaaaggta tcgccgtgcc gccggagctg gtgccgctcg
      721 ccatcgacga gttccccatc ctgttcattg ccgcagcggc cgcagccggt gaaacggtcg
      781 tcggcggtgc cgaggaactg cgcaagaagg agacggaccg tatcgccgtc atggcccgcg
      841 ggctcgaggc cgtcggcgtc aaagtcgagg agcggcccga cggtgcccgc atcatcggcc
      901 gcggcgcctt gcggggcggc agcgtcgaca gccgcggcga tcatcgcatc gccatgtcgt
      961 ttgccgtcgc gagcctgaag gcgagctcag cgatcgagat cctcaacacc gccgaggtag
     1021 ccacgtcgtt cccgaacttc ctcgacgtgg cagccgccgt cggcctgcgg gtctccgccg
     1081 ccgaggacag cgcgtgaagg gaaccagcga ctcggctccc gtcgtagcga tcgacggtcc
     1141 cagtggctcc ggcaaaggga ccatcggcag gctgctggcg cagaggctcg gctggcacta
     1201 tctcgatacg ggtgcgctgt accgactcgt agccctcgtg gccctgaaac gccatatcga
     1261 cttcggcgac gcaccggcgc tggcgcgagc ggcggcgacg ctcgacgtgc ggttcgctcc
     1321 ggcggcggcg ggcgagcgcg tccatctcga cggagtcgag gtcggcggcg agctgcgcac
     1381 ggaacgcacg ggcgatgcgg cctcgaaggt tgccgtcgtg ccagaggtgc gcgccgcgct
     1441 attacaaagg cagcgcgatt ttgccctccc tcccggcctc gtggccgatg gccgcgatgt
     1501 cgggaccgtt gttttcccgg acgccctctt gaaggtggtg ctgacggcca gcgccgaggc
     1561 gcgcgcgatg aggcgtcata agcagttgaa agaaaagggg attgatgtta gcctgcccga
     1621 cctttcttgg gacatagcgc agcgggacgc gcgcgacgcg aaccgcacgg tggcgccttt
     1681 caagccggca ccggatgcgc aggtcatcga ctccacgagt ctgactcccg aggaagtcgt
     1741 cgcccacatt ttgagtcggc tcgaaggtgt gggcgtcgtc gcgcaacgag ccaaaaagta
     1801 gcggcggctg gatgctgccg ttggtcgtca acaacccgct acatgcagga tgcagggcgg
     1861 tctgcggaag gaatcatgag cgaatcgttt gctgagctgc tagaagaaag ttttgccaac
     1921 cagaagatca agaccggtgc cattctcacc ggcatagtcg tcggcgttaa cgccgacatg
     1981 gtgatcgtca acgccgggct gaagtccgag gcggtcatcc cagccgatca attcttcaac
     2041 gagcgcggcg agctcgaagt caaagtcggc gaccacgtcg aagtggcgct cgacaccgtc
     2101 gaggacggct tcggcgagac gcgcttgtcg cgcgagaaag ccaagcgcac tcgcacttgg
     2161 acgcggctcg aggagtcgtt ccagaagggc gagatcgtca cgggcacgat cagcgggcga
     2221 gttcgcggcg gtttcaccgt cgacatcgat ctcgtgcgcg cgttcttgcc gggctcgctc
     2281 gtcgacgttc ggccgatccg cgatccggcc gccctcgaag gctcgacgct cgagttcaag
     2341 gtcatcaagc tcgaccagaa gcgcaataac gtcgtcgtgt cgcgccgtgc cgtcgtcgag
     2401 gccgagtaca gcgccgagcg cgagtcgctg ctgaagaacc tccaggaagg caccgtgctc
     2461 aagggtgtgg tcaagaacct cacggactac ggcgcgttcg tcgacttggg cggtatcgac
     2521 gggttgctgc acatcacgga catggcgtgg aagcgcgtca agcatccgtc cgaggtcgtc
     2581 agcgtcggac aggagctcga ggtcaaggtg ctgaagttcg accgcgagcg caatcgcgtc
     2641 tcgttgggtc tgaagcagct cggcgccgat ccttgggccg acctcgctcg ccgttatccg
     2701 ccgaacacac gcttgttcgg caaggtcacg aacatcgccg actacggctg cttcgccgag
     2761 atcgaagaag gcgtcgaagg cctggtccac gtgtccgaga tggactggac caacaagaac
     2821 gtcaatccgc acaaggtcgt gcaaatcggc gatgaggtcg aggtcatggt cctcgacatc
     2881 gacgaggagc gccgccggat ctcgctcggc ctcaagcagt gcaagccgaa cccgtgggtc
     2941 gagttcggcg agaactacaa gaagggcgac aaggtcgccg gccagatcaa gtcgatcacg
     3001 gactttggcg tgttcatcgg tttgccgggc ggcattgacg gcctcgtgca tctgtcggac
     3061 ctctcgtggg acgtggccgg cgaggaagcc gtgcgcaact acaagaaggg cgaggagctc
     3121 gagacgacgg tgcttgcgat cgatcccgag cgcgagcgca tctcgctcgg cgtcaagcag
     3181 ctcgacaagg atccgctgtc gttgttcttg ggcgaccacc cgaagggcag catcgtgtcc
     3241 ggcgtcgtgc aggagctcga cgcgaaggtt gcgaccatcg cgctcgaggg cggcatgatc
     3301 gggcacttgc gcgcctcgga gatcgctcgc gatcgtgtcg aggatgcacg caccgtgctc
     3361 aaggtcggcg aaaccgtcga ggcgagaatc accggcgtcg atcgcaagac gcggatcatt
     3421 tcgctgtcga tcaaggccaa ggaagtgcac gaagagcagc aggctctgca gaactacaag
     3481 agtgagacca gccgcacgtc gaccggcacc acgcttggcg atctgctcaa ggaacagatg
     3541 aacccgacgg aataagaggt cttcgcgcgt cggcgcgagg cgtgccgtgg tcgagtaaaa
     3601 ggtcctactg atgacaaaat ccgagctgat cgagtcaatc gcccgcagtc aaaaacactt
     3661 gcctgcgaag gacgtagagc tcgccgtcaa gcacatcctc gagctcatga gtgaggcgct
     3721 cgccaacggt gaacgaatcg agattcgtgg gttcggttca ttctcgctgc actatcgccc
     3781 gccgcgtatg ggacgcaatc ccaaaaccgg cgaggcggta gcgctggcgg gcaaaaacgt
     3841 tccgcacttc aaacccggca aagatcttcg cgagcgagtc aacgagtcca ggcatctccc
     3901 ggtgcgcgac taacgggtgg tgccggtgtt acggtttcgg tttcgagtac tgaaccgaga
     3961 cagtaacacc ggcacctctt agaggaccgc actttggtaa cgttgcgccg gctcggattc
     4021 gtcgctgttc ttctcgtttt gatgctcatg acgggcgtct tcgcttacag caatcccgag
     4081 tcgatcgaca tcgatatcgg cctcaagcgt ttcgagcagg tgccgatggc ggccgcgttt
     4141 gcaattgtat tggccgtcgg ttggctgctt ggtcttctgt cggccgcaat cgcgctctgg
     4201 cgctccgccg gcgagaagcg ccgcctcaag caggacctga agtacgccga ggccgagctg
     4261 cgcacgcgcc agccctccgc gtagatcgac tgcgatgttc cctatcgacg tacctaccaa
     4321 cgctacgttt ctcctcgccg cgttgttcct gctgtt
//
LOCUS       DATLEE010000729         3378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8856276, whole genome shotgun sequence.
ACCESSION   DATLEE010000729 DATLEE010000000
VERSION     DATLEE010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8856276"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VM692_16000"
     CDS             complement(<1..380)
                     /locus_tag="VM692_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53732.1"
                     /translation="MLARVDYVGGACLFALLAAATTRISDSSVGYALLAVVLPTALVA
                     IVASRKLIQPQGIAALGTWLVCGVLVPIVVVIGFMEANFLQTWIVNGHARARSLYWVS
                     VAVGALITVPVGLVGHVLLRWLISR"
     gene            complement(596..3202)
                     /locus_tag="VM692_16005"
     CDS             complement(596..3202)
                     /locus_tag="VM692_16005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ53733.1"
                     /translation="MSPSKPVARIATARRLAKRAPSSRLADERLQSVIELAADFYWEQ
                     DEQHRFTLYRPSGEPDADLEGLVGKTSWEFFAESAEAISWDAFAATLEQRAPFHDVIH
                     CLSTQANGQRYLSFSGQPVFDQRNKLKGYRGIARDVSAQMRADRLAKLDHTIAHALAE
                     AEDLASGLATVIRAMCEAQTWTAGNFWSVDPQRDTLWHEVGWNAHGGDRRSVLYPGDR
                     LPTWLSHGPVWIGDVIRDPRTSRLERAEATGWNTGLVIPVKTGGTTIGVLDFYAPHVA
                     APEPQFLKVLRSASSEVGHFFQRSLALEKLRESEERFSSTMKLAAIGIAHVEEGGRFK
                     YVNPQLCDMLGYTEQELLAMTVKEVSHPGDANMTDELRDQLRSGAIQSFKMEKRYLRK
                     DGAAIWVGLTIACKRDRAGRCQYDISIVEDISARKGAEDRIQYLATHDGLTGLPNRVM
                     FTQLLGLSIETARRYGRRLAVLFIDLDRFKVINDTLGHEAGDVLLREVAARLRECLRA
                     SDVVARLGGDEFVVLLQEINDPAQAAAVARNILNVVMKPVVILAQECRVTASIGVCLH
                     PDDGQDDQAIMKNADMAMYLAKEEGKNNFQFFTSRMKPHSIERLALEANLRRALELNE
                     FSLHYQAKVNFKSGAITGVEALLRWQNPELGSVSPARFIPLAEETGLIVPIGKWVLRT
                     ACAQTVAWQKQGLPPVRMSVNLSMRQMNDEGLVREIEAVLAETGMAPEMLELEVTEST
                     IMHNAERAVRMLTAIKDLGVRLAIDDFGTGYSSLAHLKRFPIDTLKVDRSFIREVPND
                     AEDKAIAEAIIAMGKTLSLTVVAEGVETPEQQAFLSERLCDEMQGYYFSTPVAAQDFA
                     ELLRGHSPKPLR"
BASE COUNT          609 a         1091 c         1056 g          622 t
ORIGIN      
        1 cggctgatca gccaccggag cagcacgtga ccgactaacc ctaccgggac agtgattagg
       61 gcgccgactg cgacgctaac ccagtagagg gagcgagccc gcgcatgtcc gttaacgatc
      121 caggtttgga gaaagttcgc ctccataaag ccgatcacca caacgatagg cacgagcaca
      181 ccgcacacca gccacgttcc taacgcggct atgccttggg gctggatcaa cttcctggag
      241 gcgacgatcg cgaccaatgc cgttggaagc acgaccgcga gcagggcata gccgaccgag
      301 ctgtcgctga tccgagttgt tgcggccgcc aacaacgcaa agagacacgc gccgccgacg
      361 tagtcgacgc gagccagcaa tacgcgattc ctaaggacag tcacgcgcca cctttggtgg
      421 atccgtataa cttagatgta tacgacagaa gtgtcccgtt agaaccggac agaagtgtcg
      481 tatatctcca gatgaaagca ctggcaaccc gcgccgcgcc ttggctcggt gttcatatat
      541 ttcagatatc agggaaaaag caaacgcgac gcgtagatgc ccgagtattc ggaagctagc
      601 gcagcggctt cggcgagtgc ccgcgcaaga gctcggcgaa atcctgcgca gcgactggcg
      661 tgctgaagta gtagccctgc atctcgtcgc agagccgctc gctcaagaac gcttgctgct
      721 ccggcgtctc gacgccttcg gcgacgaccg tgaggctgag agtttttccc atcgcgatga
      781 tggcttccgc gatggctttg tcctcggcgt cgtttggcac ttcgcgaatg aacgagcggt
      841 cgactttcag cgtgtcgatc gggaagcgtt tcaggtgcgc gagtgacgag tagcccgtac
      901 caaagtcatc gatcgcgagg cgcacaccca gatccttgat cgccgtcagc atgcgcacgg
      961 cgcgctcggc gttgtgcatg atcgtgcttt cggtgacctc gagctccaac atttcgggcg
     1021 ccatgcccgt ttcggcgaga accgcctcga tctcgcgcac gaggccctcg tcgttcatct
     1081 gccgcatcga caaattcacg gacatgcgta cgggcggtaa cccttgcttc tgccacgcga
     1141 ccgtctgtgc gcacgccgtg cgcagcaccc atttgccgat cggcacgatg aggcccgtct
     1201 cctccgcgag ggggatgaac cgggccggcg acaccgagcc gagctcgggg ttctgccagc
     1261 gcagcagcgc ctcgacgccc gtgatcgcgc ccgacttgaa gttgaccttg gcctggtagt
     1321 gcagcgaaaa ctcgttgagc tcgagcgcgc ggcgtaggtt cgcctcgagc gccaggcgct
     1381 cgatcgaatg cggcttcatg cggctcgtga agaactggaa gttgttcttg ccctcttcct
     1441 tcgcgaggta catcgccatg tcggcgttct tcatgatcgc ctggtcgtct tgcccgtcgt
     1501 cgggatgcag gcagacgccg atgctcgccg tgacgcggca ttcctgcgct aaaatcacga
     1561 cgggcttcat gacgacgttc aggatgttgc gtgcgacggc cgcagcctgc gccgggtcgt
     1621 tgatttcctg cagcagcacg acgaactcgt cgccgccgag gcgcgccacg acgtcgctgg
     1681 cgcgcaggca ctcgcgcaac cgcgccgcga cctcgcgcag cagcacgtcg ccggcctcgt
     1741 gccccaacgt gtcgttgatg actttgaagc ggtcgagatc gatgaacagc accgcgagcc
     1801 gtcggccgta gcgccgcgcc gtctcgatcg acaaccccag cagctgcgtg aacatcacgc
     1861 ggttcggcag gcccgtgagc ccgtcgtgcg tcgcgagata ctgaatacga tcctcggcac
     1921 ccttgcgagc cgagatgtcc tcgacgatcg agatgtcgta ctggcagcgg cccgcacgat
     1981 cgcgcttgca cgcgatcgtc aatccaaccc aaatcgccgc gccgtccttg cgcagatagc
     2041 gcttctccat cttgaacgat tggatcgcac ccgagcgcag ttggtcgcgc agctcgtcgg
     2101 tcatgttggc gtcgccaggg tgcgaaacct ccttgaccgt catcgccagc agctcttgct
     2161 cggtatagcc cagcatgtcg caaagctgcg ggttcacgta tttgaaacgc ccgccctcct
     2221 cgacgtgcgc gatgccgatc gccgcaagct tcatcgtcga cgagaaacgc tcctcgctct
     2281 cacgtagctt ctcgagcgcg agcgagcgtt gaaagaagtg accgacctcg gagctcgcac
     2341 tgcgcaacac cttcagaaat tgcggctcgg gcgccgcaac gtgcggcgcg tagaaatcga
     2401 ggacgccgat cgtcgtgccg ccggtcttga cgggaatgac gaggcctgtg ttccagcccg
     2461 tcgcctcggc gcgctcgagg cgcgaggtgc gcggatcgcg gatcacgtcg ccgatccaga
     2521 cgggtccgtg gctgagccaa gtcggcagtc gatcgccggg atagagcacc gagcgccggt
     2581 cgccgccgtg ggcgttccag ccgacttcgt gccacagcgt gtcgcgttgc gggtcgacgc
     2641 tccagaaatt gccggccgtc catgtctgcg cttcgcacat cgcgcgaatg accgtcgcga
     2701 gccccgatgc caagtcctcg gcctcggcga gtgcgtgtgc gatcgtgtga tcgagcttcg
     2761 ccaagcgatc ggcgcgcatc tgtgcgctga cgtcgcgcgc gatgccgcga tagcctttga
     2821 gcttgttgcg ttgatcgaaa acgggttggc cgctgaagct caagtaacgc tggccgttgg
     2881 cttgggtgct gaggcaatgg atgacgtcgt ggaacggcgc acgctgctcg agcgtcgcgg
     2941 cgaacgcgtc ccagctgatg gcttcggcgg attccgcgaa gaattcccag ctcgtcttgc
     3001 cgacgagccc ttcgagatcg gcatccggct cgccgctcgg ccggtacagc gtgaagcggt
     3061 gctgctcgtc ctgctcccag tagaagtcgg ccgcgagctc gatcacggac tgcagccgct
     3121 cgtcggccaa gcgcgagctt ggtgctcgct tcgcgaggcg tcgcgccgtg gcaatgcgcg
     3181 caacgggttt gctcggactc atatagatcc cgcccaaatg ctcccaaacg cgcgcggacg
     3241 tgccagcttc aggagtcatt tccggcgctc gagaggtcag ccctcgagca ggccgggacg
     3301 gtcgcgcagc ggtgagaata ccttgatggg ccatggccca attgtggcct cgctctcact
     3361 cgcagaccgc cgagtgcg
//
LOCUS       DATLEE010000730         3375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3658802, whole genome shotgun sequence.
ACCESSION   DATLEE010000730 DATLEE010000000
VERSION     DATLEE010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3658802"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..422
                     /locus_tag="VM692_16010"
     CDS             231..422
                     /locus_tag="VM692_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53734.1"
                     /translation="MSKYPQARDVYECQLYPGQTCRVVAVVNAQVTFQWLGDYARVAP
                     QVVPVNRFVVDFKLKSPQP"
     gene            468..1238
                     /locus_tag="VM692_16015"
     CDS             468..1238
                     /locus_tag="VM692_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53735.1"
                     /translation="MRSYSRGWPALAAALAVTSCGTEEGSSTALAPADEAVALALYAG
                     GPRTPAGFSTDPVPPGYAQVTTYQVKSSQLATPAATTFELCTDDWSTALAWSEEVAAQ
                     ANPYLDLVANDTTARYFEFGRVPRGTTDRYVRQRVFRCAYLDRAAVDLAAGAGNAGML
                     NARPLDAAALRDLAEYLWLFTSYNNAGNAVLATESVLPGPAHVLTLASLERGASCDRV
                     TVREWAHSADAATGALQLSNSVVREFGVRETGGALAAC"
     gene            complement(1245..1676)
                     /locus_tag="VM692_16020"
     CDS             complement(1245..1676)
                     /locus_tag="VM692_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53736.1"
                     /translation="MKWWMRIVGTFYLLEGVGLTAAAIADPAQFAAIWATSEAGALDA
                     IAVRGILVAGMPGVLTWVLLGSMLWLLSRAPAKARLLIITAVAWELVAWLPLDVIALF
                     NGFEAPRAAALIATHLLIAISGLVLLRRMPAPSPSGDFQGS"
     gene            complement(1710..>3375)
                     /locus_tag="VM692_16025"
     CDS             complement(1710..>3375)
                     /locus_tag="VM692_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53737.1"
                     /translation="KLELGATLIDEGATVGDTRLTGTDLRFRPRDALELRAEVARTTS
                     DDPLRAPAAQAYLAEIEHVTERLDVQAYLREQDAGFGVGQQLRTETGTRKVGVDARSS
                     LTQLWTARGEAFRQTNLATSADRALVSAEARRETDDATASVGVKRVVDDLPQSGVQSS
                     ELLSVGGSLDVLRDRVTLRALTEQSLGDPAASLDFPERTALGLDYHLSGATTLFAEVE
                     DAKGALLESTTTRAGVRSTPWSGSQLSSSVNREFGEYGPRVFANVGLTQTFKVGAAWA
                     MDFGVDRSDTLTGATAERFNANVPLVAGSVGGDFLATYVGAQYRADVWTITSRVERRD
                     SALAERWSFTSGFFREPIEGRALSVTTRWLDNAMAAGHGRTGDARVAYAYRPQDSKLI
                     VLERLDLEQDERVDAATSFDTKRFVNNVNLHWQFDNRFEFGTQLGARYTKSTIDGQPY
                     SGWSTLLGLDLRRDLTRVLDFGVHGTQLASQAGGTTQRSVGLDVGINSARNLWISVGY
                     NFEGFRDEHFDASRYTADGPYVRFRFKVDQDTFKDLDLSVLRPANR"
BASE COUNT          524 a         1157 c         1165 g          529 t
ORIGIN      
        1 gaccgcggcc cggggtccgg gacggcgaag cgctgcgcca aggcgggtga cgcggcgaac
       61 gagagagctg acagcgccaa cagagcggtg aggtgcatgc ggctcatggt ttcaggctcc
      121 gatgccgaat cttgggcgcg aaacgggccg aagtcacgat tttcgcgggc cgcgggtcaa
      181 gctgccgccc ggtacagtcg atgttatgtc gtcggcaccg tctgcaggcc atgtcgaaat
      241 acccacaagc acgcgacgtc tacgaatgtc agctctaccc ggggcagacc tgccgcgtcg
      301 tggcggtcgt caacgcgcag gtcacgttcc agtggctcgg cgactacgcg cgcgtcgctc
      361 cgcaggtcgt gcccgtcaac cgcttcgtag tcgacttcaa gctcaagtct ccgcagccgt
      421 agccgataac tcgccctgcg acacacgtac gacaaggacg agtacgcatg cgctcgtatt
      481 ctcgcggctg gccggctctg gccgccgcgc tagccgtcac ctcatgcggt accgaagagg
      541 gttcttcgac ggcgttggca ccggccgacg aggctgtcgc gctcgcgctg tacgcgggcg
      601 gcccgcgcac gccggcgggg ttttcaaccg atccggttcc gcccggttac gcgcaggtca
      661 cgacgtatca agtcaagagc tcgcagctcg cgacgccggc cgcgacgacg ttcgagctct
      721 gcaccgacga ttggagcacc gcgctcgcgt ggtcggaaga ggtcgcggcg caagccaacc
      781 cttatctcga tctcgtcgcc aacgacacga cggcgcgcta cttcgagttc gggcgcgtgc
      841 cgcgcggcac gaccgatcgc tacgtgcgtc agcgcgtgtt ccgctgcgcg tatctcgatc
      901 gcgccgccgt cgatcttgct gccggtgccg gcaacgcggg gatgttgaac gctcggccgc
      961 tcgacgccgc ggcgctgcgc gacctcgccg aatacctctg gctgttcacg agctacaaca
     1021 atgctggcaa tgccgtgctc gcgaccgaga gcgtgttgcc gggcccggcg cacgtgctaa
     1081 cgctcgcgag cctcgagcgc ggcgcaagtt gcgatcgagt caccgtgcgc gaatgggctc
     1141 actcggccga tgcagcgacc ggcgcgctgc agctctcgaa cagcgtagtg cgcgagttcg
     1201 gcgtgcgcga aacgggcggg gcgttggccg cgtgctgacg cgcgttacga cccttggaag
     1261 tccccggagg gggacggcgc cggcatgcgg cgcagcagca ccaagcccga gatggcgatc
     1321 agcaggtgcg tcgcgatcaa cgcggcggcg cgcggcgcct cgaagccgtt gaacagcgcg
     1381 atcacgtcga gcggcagcca cgcgacgagc tcccatgcga cggcggtgat gatcagcagc
     1441 cgcgccttcg cgggagcgcg cgagagcagc cacagcatgc tgccgagcaa gacccaggtc
     1501 agaacgcctg gcattcccgc gaccaagatg ccgcgcacgg cgatcgcgtc gagcgcgccg
     1561 gcctccgacg tcgcccaaat cgcagcgaac tgggcgggat ctgcgatcgc agcggccgtc
     1621 agtccgacgc cctcgagcag atagaacgtg ccgacgatcc gcatccacca tttcatggcg
     1681 cccccctcac gtgccgacgc ggcgtgattc tagcggttgg cgggccgcaa caccgagagg
     1741 tcgaggtcct tgaaggtgtc ctggtcgacc ttgaagcgga atcgcacgta cgggccgtcg
     1801 gccgtgtagc ggctcgcgtc gaagtgctcg tcgcggaagc cttcgaagtt gtagccgacc
     1861 gagatccaga ggttgcgcgc cgagttgatg ccgacgtcga gcccgacgga gcgctgcgtc
     1921 gtcccgccgg cttgcgatgc gagctgcgtg ccgtgcacgc cgaagtcgag cacgcgcgtg
     1981 agatcgcgcc gcaagtccaa gccgagcagc gtcgaccatc ccgagtacgg ctggccgtcg
     2041 atcgtgcttt tggtgtaacg cgcgccgagc tgcgtgccga attcgaagcg gttgtcgaac
     2101 tgccagtgga ggttgacgtt gttgacgaag cgcttcgtgt cgaagctcgt cgccgcgtcg
     2161 acgcgctcgt cctgctcgag gtcgaggcgc tcgagcacga tgagcttgct gtcctgcggc
     2221 cgataggcgt acgccacgcg tgcgtcgccg gtgcggccgt ggccggccgc catcgcgttg
     2281 tcgagccagc gtgtcgtgac ggacagcgcg cggccctcga tcggctcgcg gaagaagcca
     2341 ctcgtgaacg accagcgctc ggcgagcgcg gagtcgcgcc gctcgacgcg cgacgtgatc
     2401 gtccagacgt cggcgcggta ctgcgcgccg acgtaggtcg cgaggaagtc gccgcccacc
     2461 gagcccgcta cgagcggcac gttcgcgttg aagcgctcgg ccgtagcgcc cgtcagcgtg
     2521 tcgctgcggt cgacgccgaa gtccatggcc caggctgcgc cgaccttgaa ggtctgtgtg
     2581 agcccgacgt tggcaaacac gcgcggcccg tactcgccga actcgcggtt cacgctcgac
     2641 gacagctgcg agcccgacca cggcgtgctg cgcacgccgg cgcgcgtcgt cgttgactcg
     2701 agcagcgcgc ctttcgcgtc ctcgacctcg gcgaacagcg tagtcgcgcc cgagaggtga
     2761 tagtcgaggc cgagcgccgt gcgctccgga aaatcgaggc ttgccgcggg gtcgccgagg
     2821 gattgctccg tcagcgcgcg cagcgtcact cggtcgcgca gcacgtcgag gctgccgccc
     2881 acgctcaaga gctccgagct ctgaacgccg ctctgcggca gatcgtcgac gacgcgtttg
     2941 acgccgacgc tggccgtcgc gtcgtcggtc tcgcggcgcg cttcggccga gacgagcgcg
     3001 cgatcggcgc tcgtcgcgag attggtttgc cgaaacgctt cgccgcgcgc cgtccacagc
     3061 tgtgtcagcg agctgcgcgc atcgacgccg actttgcgcg tccccgtctc cgtgcgcagt
     3121 tgctggccga cgccgaagcc cgcgtcttgc tcacgcaagt acgcctgcac gtcgaggcgc
     3181 tcggtcacgt gctcgatctc ggcaagatag gcctgcgcag cgggcgcgcg cagcggatcg
     3241 tcggacgtcg ttcgcgcgac ctcggcgcgc aactcgagcg cgtccctcgg ccggaatcgc
     3301 aagtccgtgc ccgtgagccg cgtgtcaccg accgtcgcgc cttcgtcgat cagcgtcgcg
     3361 ccaagctcga gcttg
//
LOCUS       DATLEE010000731         7141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3660319, whole genome shotgun sequence.
ACCESSION   DATLEE010000731 DATLEE010000000
VERSION     DATLEE010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3660319"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VM692_16030"
     CDS             <1..1242
                     /locus_tag="VM692_16030"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ53738.1"
                     /translation="RQLLDAGANVDSPNGDGETALMLAAHIGALDVAKLLVERGADVN
                     AKEAWRDQTALIWAVDGNFPELTQFLIDSGADVNVRASANDWGSQITAEPRAQYRPVG
                     GLTALLYAARAGCTRCVRSLLAAGADIDRPTPEGMTPLMIAIDNQAYDTARLLLDEGA
                     NPHIWDWYGRTALYVAVDMSSFRGRLSHPTSSSETSANSIVEKLLDAGVNPNPQLNMH
                     RPSRGGNIGRFADELLNTGTTPLLRAAIGLDVETARNLLEHGALVDLPSIHGITPLMA
                     AAGHASGRGLGDQRFDPLQAQGRAIALIDLFLAAGADLNAKITDDSSRNANVGRQPNA
                     VTERKGQTALYGAVKNGWAEVVEHLLAKGADATISDALGRTPVDAALGRIAGRGTVVS
                     EEIAAMLRAAGAKEGSPVAAR"
     gene            1318..2034
                     /locus_tag="VM692_16035"
     CDS             1318..2034
                     /locus_tag="VM692_16035"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HUQ53739.1"
                     /translation="MRTVYAVLLSSIAGAALAQAPAAPPDPFVGGAALGYLSTSGNTE
                     ASSLNSLLKITWDVDGPWKHNWTALAIKASSNEVTTAEAYAAGYKATRDFSATSYLFF
                     SGDWREDRFSGYDRQVTEAVGYGRRLLDTPRHMLALEGGVGAKQSDLITGAELDEAIV
                     RGALDYLLTLSENASFNQRVLIEQGDDNRYTESTSALKTRIVGNLALVLSYVIKSNSD
                     VPVGIEKTDRFTALSLEYGF"
     gene            2260..3363
                     /locus_tag="VM692_16040"
     CDS             2260..3363
                     /locus_tag="VM692_16040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUQ53740.1"
                     /translation="MSCKECRDPLSRVESVQPAAVAAALLSLTLLLGACDKSAPEARG
                     AVAPATAGPIPVITQPARVEPMGIEIEAVGTTQANESVEVTSKASNTVTAIRFREGEI
                     VERGAVLVEMDASQARASLAEAQAALARSKSQYDRSRDLQSRQAMSMADLEQVEASLK
                     ADEARVAAAQARLDDTVIRASFHGRTGFRHVSVGSFVSPGTLITTLDDTSVIKLDFTV
                     PETYLFVLSRGLAVKAEATGLPDRVFEGTVTNMDSRIDAVTRSVTVRAELANPDGLLR
                     QGMFMTVSLHGELTPTLLVPEEALVPERGKAYLFVVNDNVVERREVHTGKRRPGFVEI
                     VNGVAEHERVVVDGTQNVRDGSKVQESSPSGAS"
     gene            3367..6519
                     /locus_tag="VM692_16045"
     CDS             3367..6519
                     /locus_tag="VM692_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUQ53741.1"
                     /translation="MRIAELSVRRPVFATVLSMLLVIFGLVSLQRLSVREYPDIDRPT
                     VSITTAYRGASAAIIETKITQVIEDSVAGIEGILKLESDSEDERSQIRIEFDINRDVD
                     AAANDVRDRIARVLRGLPPEADPPQVVKADANADAVVTLAFSSDTMSMLDLTDFAERN
                     IVDRMSTVPGVASVGIQGGRRYSMRVWIDRQALAARQMTVTDIEDALRRENVELPAGR
                     LESRTREFSLRTLVGLETEQDFRNLVIERGADGHLVRLGEVADVRLAAENERSYSRLN
                     GVPGLMMQVQAQSKGNTLDIARGVRAEVARIQESLPEGTTLNVSVDNALPIEAALREV
                     LIAVAFALTSVLAVIYIFLGNIRATLIPAVTIPVSIVASLTVMYALGYSINVLTLLGL
                     VLAIGLVVDDAIVVLENAHRRHELGEPTLVAAITGTREIGFAVIATTLTLISVFVPIS
                     FLPGDLGRLFREFGFTLAASVAFSALIALTLTPMLASRLPEQERRNRFAAMMDRFFRA
                     LARFYERCLRITVAHPWPVVGVLAVLIGVGVVTFRSVPAEFTPQADNGRAFIGIEGPE
                     GSSFEYIDRYARQLETIALKAIEGGEVDRVLIRVPGQGGNDLRTGDINTARAFLILAD
                     WDDRTRTAQEIIQPVLAEARKLPGVRVSMGMPGGLGRGGLQSPVVAVIGGPDYERLAV
                     WSDQMLQLAQANPGLANVQSNYKERKPQIRVAIDRNRAADLGISLESVGRTLETVLGS
                     RIVTTYIDRGREYNVILQGNDEIRETTTDLTNIRVRSTRTAELIPLSSIVRLEETSGT
                     MQLARFDRLRAIKISADLANGYTMGEAVKWFEDTIAKELPPEATLDWDGQTREFTRTG
                     QQLYYTFLLALAVVYLVLAAQFESFVHPIIIMVTVPLALLGAVFGLKLFGLTVNIFSQ
                     IAVIMLIGIAAKNGVLIVEFANQLRDRGLEFVDAVVQAAVTRLRPVLMTSLCTAFGSL
                     PLMLASGAGSEQRRPIGIVVFFGMTVAVFLTLFAVPTVYALLARKTKSPQHVSRLVDR
                     LMGAPGGAGGEPVLHDLRRD"
     gene            complement(6591..>7141)
                     /locus_tag="VM692_16050"
     CDS             complement(6591..>7141)
                     /locus_tag="VM692_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HUQ53742.1"
                     /translation="TYADLVAVPDNFVAEILAGELYASPRPIFTHMRVASALGVLLGG
                     PFQFRINGPGGWLLVDEPELHLGADVLVPDLAGWRTEKAAAASAAAYPTVAPDWICEV
                     LSRSTETIDRGHKLRIYEREAVGHIWLMDPVSQTLEVMQRARRRWSLLATHRGGQRVR
                     AAPFEAVELELAAVWVSEAERT"
BASE COUNT         1195 a         2483 c         2349 g         1114 t
ORIGIN      
        1 cgccagctgc tcgacgcagg cgcgaatgtc gactcgccga acggcgacgg cgagacggcg
       61 ttgatgctgg ccgcgcacat cggtgcgctc gacgtcgcga agctgctcgt ggagcgcggc
      121 gccgacgtca acgcgaaaga agcgtggcgc gatcaaacgg cgctgatctg ggccgtcgac
      181 ggcaacttcc ccgagctcac gcagttcctg atcgacagcg gcgccgacgt caacgtgcgg
      241 gcctcggcca acgactgggg ctcgcagatc accgccgagc cgcgcgcgca gtatcggccc
      301 gtcggcggtt tgacggccct gctctatgcg gcgcgcgccg gttgcacgcg ctgcgtgcgc
      361 tcgttgctcg cggccggcgc cgacatcgat cggccgacgc cggaaggcat gacgccgctg
      421 atgatcgcga tcgacaacca ggcctacgac acggcgcggt tgctgctcga cgaaggcgcg
      481 aatccgcaca tctgggattg gtacggccgc acggcgctct acgtcgccgt cgacatgagc
      541 tcgttccgcg gccgcttgtc gcacccgacg agctcgtccg agacctcggc aaacagcatc
      601 gtcgagaagc tgctcgacgc gggcgtcaat ccgaacccgc agctgaacat gcaccggccg
      661 agccgcggcg gcaacatcgg ccgcttcgcc gacgagctgc tgaacaccgg cacgacgccg
      721 ctgctgcgcg ccgcgatcgg cctcgacgtc gagacggccc gcaatttgct cgagcacggc
      781 gcgctcgtcg acctgccgag catccacggc atcacgccgt tgatggcggc ggccgggcac
      841 gcctcgggac gaggcctcgg cgatcagcgg ttcgatccgc tgcaagctca aggccgagcc
      901 atcgctttga tcgacctgtt cttggccgcc ggcgccgacc tcaacgccaa gatcacggac
      961 gacagcagcc gcaacgcgaa cgtcgggcga cagccgaacg cggtgacgga gcgcaaaggg
     1021 caaacggcgc tctacggtgc cgtgaagaac ggttgggccg aagtcgtgga acatctgctc
     1081 gcgaaaggcg cggacgcgac gatcagcgac gcgctcggca gaacgccggt cgacgccgcg
     1141 ctcggccgca tcgcgggccg cggcaccgtc gtctccgagg agatcgccgc gatgctgcgc
     1201 gccgccggcg ccaaggaagg cagccccgtc gcggcgcgct agaggtcgag tcttcgcccc
     1261 gtggcgccgg cgctgcgctg cgggtaccat gccgcccgca tctccgggag gctttccatg
     1321 cgcaccgtct acgccgtact tctcagctcg attgcgggag ccgcgctcgc gcaggcaccc
     1381 gccgctccgc cggacccgtt cgtcggcggc gccgcgctcg gctacttgtc gacgagcggc
     1441 aacaccgaag cgtcgagtct caactcgttg ctgaagatca cctgggacgt cgacggtccg
     1501 tggaagcaca attggactgc gctcgcgatc aaggcgagca gcaacgaggt gacgacggcc
     1561 gaggcgtacg cggccgggta caaagcgacg cgcgatttct cggcgaccag ctatttgttc
     1621 ttctccggcg attggcgcga agaccgcttc tcgggctacg accggcaagt tactgaagcc
     1681 gtcggctacg gccgccgcct gctcgacacg ccgcggcaca tgctggccct cgaaggcggc
     1741 gtcggtgcca agcagtctga cctgatcacg ggcgcagagc tcgacgaggc gatcgttcgc
     1801 ggtgcgctcg actatctgtt gacgctgtcg gagaacgcgt cgttcaacca gcgggtgctg
     1861 atcgagcaag gcgatgacaa ccgatatacg gagtcgacgt cggcgttgaa gacgcggatc
     1921 gtcggcaatt tggcgctcgt gctgtcgtac gtcatcaaga gcaactcgga tgtgccggtc
     1981 ggcatcgaga agacggatcg cttcactgcg ctgtcgctcg agtacgggtt ctaacgcgaa
     2041 gactgtcgtt ctggccgatg gcgacgaaga tctaactcca cccgatcgag ccgcaccgtt
     2101 cgagcgcacg cgacctggaa gttttgaggc cggtttagtc caaagttttc ttgcgtgcgg
     2161 ccggaaaccc gccatgcgcc agtcgcttac gaaggccgca tgcgaggctg tgtgctataa
     2221 atcggcttcg tctcccaggg ctcagagaac cgccgcgtca tgtcatgcaa ggaatgcagg
     2281 gacccgcttt cgcgcgtcga aagcgtacaa ccggccgcag tcgccgcggc actgctatct
     2341 ctgacgctgc tgcttggcgc ctgcgataaa tccgcacctg aagcccgggg cgccgtcgcc
     2401 cctgcgacgg cgggcccgat acccgtcatc acccagcccg cgcgcgtcga gcccatgggc
     2461 atcgagatcg aggccgtcgg cacgacgcag gccaacgaat ccgtcgaggt cacgtcgaag
     2521 gcgtcgaata ccgtcacggc gatccgcttc cgtgagggcg agatcgtcga gcgaggcgcc
     2581 gtactcgtcg agatggacgc gagccaagcg cgcgccagtc tcgcggaagc gcaagcagcg
     2641 ctcgcgcgca gcaagagcca gtacgaccgc agccgcgatc tgcaatcgcg ccaagcgatg
     2701 tcgatggccg atctcgagca ggtcgaggcc tcgctgaagg ccgacgaggc gcgcgtcgcc
     2761 gcggcgcaag cgcggctcga cgacaccgtc atccgcgcgt cgttccacgg ccgaacgggc
     2821 ttccgccacg tcagcgtcgg cagcttcgtg agcccaggca cgctcatcac gacgctcgac
     2881 gacacgtccg tcatcaagct cgacttcacg gtccccgaga cctatttatt cgtgctgagc
     2941 cgcggcctgg ccgtcaaggc cgaggcgacg gggctgccgg atcgcgtgtt cgaaggcacg
     3001 gtcacgaaca tggactcgcg catcgacgcc gtgacgcgct ccgtcaccgt gcgcgccgag
     3061 ctcgcgaacc ccgacggcct gctgcgccag ggcatgttca tgacggtatc gctgcacggc
     3121 gagctgacgc cgacgctgct cgtgcccgag gaagcgctcg tgcccgagcg cggcaaggcg
     3181 tacctcttcg tcgtcaacga caacgtcgtc gagcggcgcg aagtgcacac cggcaagcgc
     3241 cgccccggct tcgtcgagat cgtcaacggc gtcgcggagc acgagcgcgt cgtcgtcgac
     3301 ggcacgcaga acgtgcgcga cggcagcaag gttcaggaga gctcgccgag cggcgcgagc
     3361 tgacccatgc gcatcgccga gctgtcggtt cgccggccgg tcttcgcgac cgtgctcagc
     3421 atgctgctcg tgatcttcgg cctcgtgtcg ctgcagcggc tgtccgtacg cgagtatccc
     3481 gacatcgacc gcccgaccgt ctcgatcacg acggcgtatc gcggagcttc ggccgcgatc
     3541 atcgagacca agatcacgca ggtcatcgag gactccgtcg ccggcatcga aggcattctg
     3601 aagctcgagt ccgacagcga ggacgagcgc tcgcaaatcc gcatcgagtt cgacatcaac
     3661 cgcgacgtcg acgccgccgc caacgacgtc cgcgaccgca tcgcgcgcgt gctgcggggg
     3721 ttgccgcccg aggccgatcc gccgcaggtc gtcaaagccg acgcgaacgc cgacgccgtc
     3781 gtgacgctcg cgttctcgtc cgacacgatg tcgatgctcg acctcacgga tttcgccgaa
     3841 cgcaacatcg tcgaccgcat gtcgacggtc ccgggcgtcg ccagcgtcgg catccagggc
     3901 ggccgacgct actcgatgcg ggtttggatc gaccgccaag cgctggccgc gcgccagatg
     3961 acggtcacgg acatcgagga cgcgctgcgg cgcgagaacg tcgagctgcc cgcgggccgg
     4021 ctcgagtcgc gcacgcgcga attttcgctg cgcacgctcg tcggcctcga gacggagcag
     4081 gacttccgca acctcgtcat cgagcgcggc gccgacggcc acctcgtacg actcggcgag
     4141 gtcgccgacg tgcggctcgc cgccgagaac gagcgctcgt acagccgctt gaacggcgtg
     4201 cccggcctca tgatgcaggt gcaagcgcaa tcgaaaggca acacgctcga catcgctcgc
     4261 ggcgtgcgcg ccgaggtcgc gcgcattcag gagagcttgc ccgagggcac gacgctgaac
     4321 gtcagcgtcg acaacgcgct gccgatcgaa gccgcgctgc gcgaagtgct gatcgccgtc
     4381 gccttcgccc tgacgtcggt gctcgcggtc atctatatct tcctcggcaa catccgcgcg
     4441 acgctgatcc cggccgtcac gattcccgtc tcgatcgtcg cgtcgctgac cgtcatgtat
     4501 gcgctcggct actcgatcaa cgtgctgact ttgctcggcc tcgtgctcgc gatcggcctc
     4561 gtcgtcgacg acgcgatcgt cgtgctcgag aatgcgcacc gccggcacga gctcggcgag
     4621 ccgacgctcg tcgccgcgat cacgggcacg cgcgaaatcg gcttcgccgt catcgcgacg
     4681 acgctgactt tgatctccgt gttcgtgccg atctcgttcc tgccaggcga tctcggccgg
     4741 ctgttccgcg agttcggctt caccttggcg gcctcggtcg cattctcggc gctgatcgcg
     4801 ctgaccttga cgccgatgtt ggcgtcgcgc cttcccgagc aagagcgccg caaccgcttc
     4861 gcggccatga tggatcgctt cttccgggcg ctcgcgcgct tctacgagcg ctgcctgcgc
     4921 atcaccgtcg cgcacccgtg gcccgtcgtc ggcgtgctgg cggtgctgat cggcgtcggc
     4981 gtcgtgacgt tccgctccgt gcccgccgag ttcacgccac aggccgacaa cggccgcgcg
     5041 ttcatcggca tcgaagggcc cgaaggctcg agcttcgagt acatcgatcg ctacgcgcgc
     5101 cagctcgaga cgatcgcgct gaaggccatc gaaggcggcg aagtcgatcg cgtgctgatc
     5161 cgcgtgccgg gccagggcgg caacgacttg cgcacgggcg acatcaacac ggcgcgcgcg
     5221 ttcctgatcc tggccgattg ggacgaccgc acgcgcacgg cgcaagagat catccagccc
     5281 gtgctcgccg aggcgcgcaa gctgcccggc gtccgcgtct cgatgggcat gccggggggc
     5341 ttggggcgcg gcggcctgca atcccccgtc gtcgccgtca tcggcgggcc cgactacgag
     5401 cgcttggccg tctggtcgga ccagatgctg cagctcgcgc aggcgaatcc cggcctcgcg
     5461 aacgtccaga gcaactacaa ggagcgcaag ccgcagatcc gcgtcgcgat cgaccgcaat
     5521 cgcgcggccg atctcggcat ctcgctcgag agcgtcggcc gcaccctcga gaccgtgctc
     5581 ggctcgcgga tcgtcacgac gtacatcgac cgcggccgcg agtacaacgt catcttgcag
     5641 ggcaacgacg aaatccgcga gacgacgacc gacttgacga acatccgcgt gcgctcgacg
     5701 cgcaccgccg agctgatccc gctgtcgagc atcgtgcggc tcgaggagac gtcgggcacg
     5761 atgcagctgg cccgcttcga ccgcctgcgc gcgatcaaga tctcggccga cctcgcgaac
     5821 ggctacacga tgggcgaagc cgtgaagtgg ttcgaggaca cgatcgccaa ggagctgccg
     5881 ccggaggcga cgctcgactg ggacggccag acgcgcgagt tcacgcgcac gggccagcag
     5941 ctctactaca cgttcctgct cgcgctcgcc gtcgtctatc tcgtgttggc cgcgcaattc
     6001 gagagcttcg tgcacccgat catcatcatg gtcacggtgc cgctcgcgct cttgggcgct
     6061 gtgttcggcc tcaagctatt cggcctcacg gtcaacatct tcagtcagat cgccgtgatc
     6121 atgctgatag gcatcgcggc caaaaacggc gtgctgatcg tcgagttcgc gaatcagctg
     6181 cgcgaccgcg gcctcgagtt cgtcgacgcc gtcgtgcaag cggccgtcac gcgcctgcgg
     6241 cccgtgctga tgacgagcct ctgcacggca tttggctcgt tgccgctgat gctggcgtcg
     6301 ggcgccggct ccgagcagcg ccggccgatc ggcatcgtcg tgttcttcgg catgacggtc
     6361 gccgtgttcc tgacactgtt cgcggtgccg accgtctacg cgctgctcgc gcgcaagacg
     6421 aagtcgccgc agcacgtgag ccggctcgtc gatcggctca tgggcgcgcc cggcggcgcc
     6481 ggcggcgaac ccgttctcca cgacctccgc cgcgactagc gacgctcgtc gcgatgccct
     6541 ccgcggaggg cgacctcgaa ccctccgcgg agggatggtt gaaaatcacg tcaagtacgt
     6601 tcggcttccg aaacccaaac ggcagcgagc tcgagctcaa ccgcctcgaa cggcgcagcc
     6661 cgcacgcgct gcccgcctcg atgcgtcgcg agcaacgacc agcgacggcg cgcacgctgc
     6721 atgacctcga gcgtctgcga tacgggatcc atgagccaga tgtggccgac ggcctcgcgt
     6781 tcatagatgc ggagcttgtg gcctcgatcg atcgtttcgg tggagcgcga tagcacctca
     6841 cagatccaat cgggcgcgac ggttggatag gccgcggccg atgccgcggc cgccttctcg
     6901 gtgcgccaac ccgctaggtc cggcaccagg acgtcggcgc ccagatgcag ctcgggctcg
     6961 tcgacgagca accagccgcc gggaccgttg atgcgaaatt gaaaaggacc gccgagcaac
     7021 acgccgagag ccgacgccac gcgcatgtgc gtgaatatcg gccgcggcga cgcatagagc
     7081 tcgcccgcca gaatctcggc aacgaaattg tccggaaccg cgacgagatc ggcgtacgtg
     7141 g
//
LOCUS       DATLEE010000732         7255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6474095, whole genome shotgun sequence.
ACCESSION   DATLEE010000732 DATLEE010000000
VERSION     DATLEE010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6474095"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VM692_16055"
     CDS             complement(<1..232)
                     /locus_tag="VM692_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53743.1"
                     /translation="MADHTNGRDYPEPRFDSFVSKATSRRQFIKGVIFSGAAASGAGY
                     LLSMAGCSGQGGSAAGVERLLTLNVNGQMRPVD"
     gene            complement(335..2737)
                     /locus_tag="VM692_16060"
     CDS             complement(335..2737)
                     /locus_tag="VM692_16060"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HUQ53744.1"
                     /translation="MAYDLLGKNFTPPDVHAKVTGKAKYSEDFRVDGMVFLKVLASPM
                     PHARVTNIDASAALAMPGVHGVLTADEVTNAPDPGETVLTNEPLFVGHPILAVAADTE
                     ELASDALEKIKVSYEELPFTVDPLQSLFPGGPNARTNGNVANQGVDLQTVKWTAQDFA
                     AAGDTQLPAGKAVTEWSFGDVEAGLASAKLVLDETFVTAGLAHHSMEPRSALAYWQNG
                     KCILHGSTQSQSFPFPGIARRLGIDAADLSYISEFCGGGFGSKGGDYPLMVLPALMSK
                     KINRPVMMRVSRHEEYAMGSARAGFQGRIKMGFAADGRVTAVDLYIVQENGPNTGFND
                     FANGAEAVSLVYQPPAMRFRGVPVLTNTPPRGPQRGPGQNQIAAAVEPLIDKAARQLG
                     LDRVAIRRINAPDNAAKIGSMQGGVTSAYLKDALDRGAEQFDWAARQARSGQRNGSKV
                     RSVGVGTAFHTAGGNGFDGLVRITPDGKLHIHTGVGNLGTYSHTATSRVAAEVLKANW
                     DNCTVVRGDSRNHLPWNLGQFGSNTSFTMTRTNYAAAMDAVTKLKEIAAMDLGGAPDD
                     YDIGGERVFAKSDASKGLSYAQAAQRAIELGGKFDGHEVGNDLNPMTKASAAGVAGTG
                     LVGVAKDNLPRTGTVPALAAGFVEIELDVETGKFEILDYIGVADCGTVLHPQGLSHQI
                     KSGAVMGFGMAAHERHIYDPQNGLPGNVGLLQSKPVSYLDVPATMRTAWVDLPDPQNP
                     VGAKGIGEPIQGCAAAALLCAISDALGGHYFNRTPVMPDMIVNAAAKREPSHKPLQVN
                     TA"
     gene            2932..5520
                     /locus_tag="VM692_16065"
     CDS             2932..5520
                     /locus_tag="VM692_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ53745.1"
                     /translation="MAFMKRPRDARGGGLYTAQRTLLATAIASSLCLPASVLAQEGLE
                     EIVVTARFREENLQQTPLAITAITGDMLEARGATSTLDLDAFVPNAVIAPLGAGWGST
                     AAAFIRGIGLGDNSLSFEPGVPIYIDDVYHGRPQGAVLDLLDIDRVEVLRGPQGTLFG
                     KNTLGGAVRIISKKPEGSGSGFVDVQAGERDRLNFRGSYDFPIVEDKVLARISASSKK
                     QDGYFDILDYECVNGPGSLGLGGPGVAAGTNGVARPAGNPDPTNPAWIAFAANTHPAI
                     GGVRLGSVLGTTDARGCVVDHFGNENVQSGRVALRFLASDTVEVNVVADLTYSDQEGP
                     PDKYTVQLRTTDGVPPGVAAQINGWNNTIAIPVFGAGGQFDGRFQTPDKYTSYHRFGQ
                     DPLTGRITPNRNELKHTGLQVDLDWDLSDNVSFKSTTAYRDFDNSFGRDSDGTPLPGT
                     FTWDTSKHEQFTQEFQLSGITGADDGLEWTTGLFYYDAFDSNQGYNNGYVYTSSFSDH
                     KDEQDLTNYAVFAHFVWAITEKLNLSTGVRYTDDQKDATIYRRTGNTAPAGIGAPQFV
                     LIDNGLITVESQEWSPKLSFDYQFTATTMGYVQLSTGFRGGGFSPRPANALQLTAFQP
                     EFIDNFEVGVKTDWLDGRLRFNGDVYYMKNRDKQQAIADCAPCAPTRVNSFPTVNTGE
                     SRNWGLEGEILAEPVDNLRIDFSLGYQDYKVTDLGRSSGIFITVPDTTGDPSIRGDVL
                     YSPRTPEWNVGLGISYPFRVGSRGATLTPRFDYTWQDDIWFTTNPVAGIVTEEDGRQP
                     AYGVLNARVTWDAPNTMWSASAYALNLTDEYYFYGKLSLLGNSGREQGNPAPPREVGV
                     SIRFNF"
     gene            5592..6071
                     /locus_tag="VM692_16070"
     CDS             5592..6071
                     /locus_tag="VM692_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53746.1"
                     /translation="MDVGTWELLSYIVTTIGLPVAILVFLYEQKKERDNEDEEVYQLL
                     SDNYQDFLKIALGHPDLRLFTPEQTPQLNEEQQERMMIIFNMLISLFERAYLLLYSPK
                     MSSQHRRRWLSWEDYMREWCRREDFRALLPVLLDGEDPEFCAYIQALADQHPRLIVS"
     gene            complement(6076..>7255)
                     /locus_tag="VM692_16075"
     CDS             complement(6076..>7255)
                     /locus_tag="VM692_16075"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUQ53747.1"
                     /translation="ASDNGGRFGLYVVSGETGAIERPLYTHASLDVESVRVDPYTNRV
                     VGADVGPEEIVWFDVALRAQQAELDQAFRGESPRIVSWSQDRSRSIVSTESADRAAAY
                     YFLDSKAGTAKQIASTNMQLATVKLARREAYGYAARDGIKIPAYLTRPLGVEGPAPLV
                     LLPHGGPAARDFSGYDWLAHGLASRGYAVLQPNFRGSGGYGQEWEEAGHGEWGIGVMQ
                     HDLTDGVAALIAAGIADPERVCIVGASYGGYAALAGAAFTPELYRCAAAIAGVADLRD
                     MLAFERERAGGRSATVSYWRQAMGVDEEGSSSQDLDAASPARHSERVRAPVLLIHGRD
                     DTVVPIVQSRTMESALRDAGKSVQFVELEGEDHWLSGAKTRLATLQALDGFLAEHLKN
                     "
BASE COUNT         1240 a         2457 c         2339 g         1219 t
ORIGIN      
        1 cgtcgacggg ccgcatctgg ccgttgacgt tcaacgtcaa gaggcgctcg acgcccgcgg
       61 cggagccgcc ctggcccgag cagccggcca tcgacagcag atagcccgca cccgacgcag
      121 cggcgcccga gaagatgaca ccttttataa actggcgtcg cgacgtggct ttcgagacga
      181 atgagtcgaa acgcggttcc gggtagtccc ggccgttggt gtggtcagcc atggagatgt
      241 ctcccctccg aagcgccgaa gacgcggaaa atcgagttga tgctaggtga aaagccggcc
      301 ccgctcaact ggccggacgc ggtgtcgtgg ccgctcatgc cgtgttgacc tgcagcggtt
      361 tgtgggacgg ctcgcgcttg gccgccgcgt tgacgatcat gtcgggcatg accggcgtgc
      421 ggttgaagta gtggccgccc aaggcgtcgg agatcgcgca tagcagcgcc gccgcggcgc
      481 agccctggat cggctcgcca atgcccttcg cgccgacggg attctgcggg tccggcaggt
      541 cgacccaggc cgtgcgcatc gtggccggca cgtctagata agagaccggc ttcgactgca
      601 gcagcccgac gttgccgggc aagccgttct gcggatcgta gatgtgccgc tcgtgcgccg
      661 ccataccgaa gcccatgacc gcgccgctct tgatctgatg agacaagcct tgcgggtgca
      721 gcacggtgcc gcagtcggcc acgccgatgt agtccaagat ctcgaacttg ccggtctcga
      781 cgtcgagctc gatctcgacg aacccggccg cgagcgcggg cacggtgccc gtccgcggca
      841 agttgtcttt cgcgaccccg acgaggcccg tgccggccac gccggccgcc gacgccttcg
      901 tcatggggtt cagatcgttg ccgacctcgt ggccgtcgaa cttgccgccg agctcgatcg
      961 cgcgctgcgc cgcctgcgcg tagctcaagc ctttgctcgc gtcgctcttc gcgaacacgc
     1021 gctcgccgcc gatgtcgtaa tcgtccggcg ccccgccgag gtccatcgcg gcgatctcct
     1081 tgagcttcgt cacggcgtcc atcgcggcag cgtagttcgt gcgcgtcatc gtgaacgagg
     1141 tgttgctgcc gaactggccg agattccacg gcagatgatt gcggctgtcg ccgcgcacga
     1201 cggtgcagtt gtcccagttg gccttcagca cctcggccgc cacgcgcgac gtcgccgtgt
     1261 gcgagtacgt gccgagattg ccgacgcccg tgtggatgtg gagcttgccg tccggggtaa
     1321 tgcgcacgag gccgtcgaag ccgttgccgc cggccgtgtg aaacgccgtg cccacgccca
     1381 cggaacggac cttcgagccg ttacgctggc cgctgcgcgc ctggcgcgcg gcccaatcga
     1441 actgctcggc gccgcgatcg agcgcatcct tcaagtaggc gctcgtgacg ccgccttgca
     1501 tcgagccgat cttggccgcg ttgtcgggcg cgttgatacg ccggatcgcg acgcggtcga
     1561 gcccaagctg ccgcgcggct ttgtcgatca acggctcgac ggcggccgcg atctggttct
     1621 ggcccggccc gcgctgtggg ccgcgcggcg gcgtgttcgt cagcaccggc acgccgcgga
     1681 accgcatcgc cggcggctga tagacgagtg aaacggcctc ggcgccgttc gcgaagtcgt
     1741 tgaagcccgt gttcgggccg ttctcctgca ctatataaag gtctacggcg gtcacgcggc
     1801 cgtcggccgc aaagcccatc ttgatccgcc cttgaaaccc ggcgcgcgcc gagcccatgg
     1861 cgtattcctc gtggcgcgac acgcgcatca tcacgggccg attgatcttc ttcgacatca
     1921 gagccggcag caccatcagc gggtagtcgc cgcccttcga gccgaagccg ccgccgcaga
     1981 actccgagat gtaggacaga tccgcggcgt cgatgccaag ccgccgtgcg ataccgggaa
     2041 acgggaagct ctggctctgc gtggaaccgt gcaggatgca cttaccgttc tgccaatacg
     2101 cgagcgcgct gcgcggctcc atcgaatgat gcgcgagacc cgcggtgacg aacgtctcgt
     2161 cgagcacgag cttggcggac gcgagccccg cttcgacgtc gccgaacgac cattccgtaa
     2221 cggctttgcc ggccggcagc tgcgtgtcac cggcggccgc gaaatcctgc gccgtccact
     2281 tgacggtttg cagatcgacg ccctggttcg cgacgttgcc gttcgtgcgc gcattcggcc
     2341 cgccggggaa caggctctgc agcggatcca ccgtgaacgg cagctcctcg tacgagacct
     2401 tgatcttctc gagcgcgtcg ctcgcgagct cttccgtgtc cgcggcgacg gcaagaatcg
     2461 gatgaccgac gaacaacggc tcgttcgtca gcaccgtctc accgggatcg ggcgcgtttg
     2521 tgacctcgtc ggccgtcagc acgccgtgca cgcccggcat cgcgagcgcg gcgctcgcgt
     2581 cgatgttcgt gacacgcgca tgcggcatcg ggctcgccag cactttgagg aacaccatgc
     2641 cgtcgacacg gaaatcctcc gagtatttgg ctttgcccgt caccttcgcg tggacgtcgg
     2701 gcggcgtgaa atttttgcca agcaggtcgt aggccatgtg agctcccgga aaggtggatt
     2761 gcacgacgtc gttcgtcggc ccgggcatga tactccggga agcgcgcaag ccatgctcga
     2821 cgagcggccg tgaccgcgta actcgctctg cccccaacag tttccagccc ggcacggttt
     2881 gctggtagcc tcactacccg caactcactc aaacaccggg ggatcgggac catggcattc
     2941 atgaaacggc cgcgcgacgc gcgcggcggc ggactctaca cggcgcagcg cacgctgctc
     3001 gcgaccgcaa tcgcgtcgtc actctgtcta ccggcgagcg tgctggcgca agaaggtctc
     3061 gaggagatcg tcgtcacggc gcgcttccgc gaagaaaact tgcagcagac gccgctcgcg
     3121 atcacggcta tcacgggcga catgctcgag gcgcgcggcg caacgagcac gctcgacctc
     3181 gacgcgttcg tgccgaatgc cgtcatcgcg ccgctcggcg cgggctgggg ctcgacggcc
     3241 gcggcgttca ttcgcggcat cggcttaggc gacaactcgt tgtcgttcga gcccggcgta
     3301 ccgatctaca tcgacgacgt gtaccacggc cgcccgcaag gcgcggtgct cgacctgctc
     3361 gacatcgacc gcgtcgaggt gctgcgcgga ccgcaaggca cgctgttcgg caagaacacg
     3421 ctcggcggcg ccgtgcgcat catctcgaag aagccggaag gctcgggctc gggcttcgtc
     3481 gacgtgcaag ccggtgagcg cgatcggctg aatttccgcg gctcctatga tttcccgatc
     3541 gtcgaggaca aagtgctcgc gcgcatctcc gcgtcgtcga agaagcagga cggctacttc
     3601 gacatcctcg actacgaatg cgtcaacggg ccgggctcgc tcggcctggg cggtcccggc
     3661 gtggccgcgg ggacgaacgg cgtcgcgcgc cccgccggca acccagaccc gacgaatccc
     3721 gcgtggatcg cgttcgccgc gaatacccac cccgcgatcg gcggcgtgcg gcttggcagc
     3781 gtgcttggca cgaccgacgc gcgcggctgc gtcgtcgacc acttcggcaa cgagaacgtg
     3841 cagtcgggac gcgtggccct gcggttcctt gcgtccgaca cggtcgaggt caacgtcgtc
     3901 gccgatctca cgtacagcga ccaagagggc cctcccgaca aatacacggt gcagctgcgt
     3961 accacggacg gcgtgccgcc cggagtcgcg gcgcagataa acggctggaa caacaccatt
     4021 gcgattccgg tgttcggcgc cggcggccag ttcgacgggc gttttcaaac gccggacaag
     4081 tacacgagct accaccgctt cggccaagac ccgctgaccg ggcgcattac gccgaaccgc
     4141 aacgagctca agcacacggg tctgcaggtt gacctcgatt gggacctctc cgacaacgtg
     4201 agcttcaagt ccacgaccgc gtaccgcgac ttcgacaact cgttcggccg cgactccgac
     4261 ggcacgccgc tgcccggaac cttcacgtgg gatacgtcga agcacgagca gttcacgcag
     4321 gaattccagc tgagcggtat cacgggcgcc gacgacggcc tcgagtggac gacggggttg
     4381 ttctactacg acgccttcga ctcgaaccag ggttacaaca acggttacgt ttacacgtcg
     4441 agcttctccg accacaagga cgagcaggac ctcacgaact acgcggtgtt cgcacacttc
     4501 gtttgggcca tcaccgagaa gctgaatctg tccaccgggg ttcgctacac cgacgaccaa
     4561 aaggacgcga cgatctaccg cagaacgggc aatacggcac ccgccggcat cggggctccg
     4621 cagttcgtgc tgatcgacaa cggcttgatc accgtcgagt cgcaagagtg gtcgccgaag
     4681 ctttccttcg actatcaatt cactgcgacc accatggggt acgtgcagct atcgacgggc
     4741 ttccgtggcg gcggcttcag cccgcgtccg gccaacgcgc tgcagctgac ggcgttccag
     4801 cccgagttca tcgacaactt cgaggtcggc gtgaagaccg actggctcga tgggcggctg
     4861 cgcttcaacg gcgacgtcta ctacatgaag aacagggaca agcagcaagc gatcgccgac
     4921 tgcgcgccgt gcgcgccgac gcgcgtgaac tcgttcccga ccgtgaacac gggcgagtcg
     4981 cgcaactggg gcttggaggg cgagatcctg gcggagccgg tcgacaactt gcggatcgac
     5041 ttctcgctgg gctaccagga ctacaaggtc acggacttgg gccgctcgtc cggcatcttc
     5101 atcacggtgc ccgatacgac cggggacccc tcgatccgcg gcgacgtgct gtactcgccg
     5161 cgcacgccgg aatggaacgt gggactcggc atctcgtacc cgttccgcgt cggcagccga
     5221 ggcgcgacgc tgacgccgcg tttcgattac acctggcagg acgacatctg gttcacgacg
     5281 aaccccgtgg cgggaatcgt gaccgaggag gacggccggc agccggctta cggggtgctg
     5341 aacgctcgcg tcacttggga tgcgccgaac acgatgtggt cggcctcggc ctatgcgctg
     5401 aacctgaccg acgagtacta cttctacggc aagctgtccc tgctcggcaa ttcgggccgc
     5461 gagcagggca atccggcgcc gccgcgtgaa gtcggcgtca gcataaggtt caatttctaa
     5521 gcacgagacg tgggaacatg tgcgggcgcg ttcggcgacg aacgcgccct ttttttgcgc
     5581 ggagatcggg cgtggacgtc ggcacttggg aactgctgag ctacatcgtc acgacgatcg
     5641 gcttgcccgt agcgatcctc gtgttcctct acgagcagaa aaaggagcgc gacaacgagg
     5701 acgaggaggt ctatcagctg ctctcggaca actaccaaga tttcctgaag atcgcgctcg
     5761 gccatcccga cctgcgcttg ttcactccgg agcaaacgcc acagctcaac gaggagcagc
     5821 aggaacgcat gatgatcatc ttcaacatgc tgatctcgct gttcgagcgc gcgtacctgc
     5881 tgctctactc gccgaagatg tcgtcgcagc accggcggcg ctggctgagc tgggaggact
     5941 acatgcgcga gtggtgtcgg cgcgaagact tccgcgcgtt gctacccgtg ctgctcgacg
     6001 gcgaggaccc tgagttctgc gcttacattc aagcgctcgc cgaccagcat ccgcgcctga
     6061 tcgtcagttg agccgtcagt tcttgaggtg ctccgcaaga aacccgtcca aagcttgcag
     6121 cgtcgcgagc cgcgtcttcg cgccggacag ccagtgatcc tcgccttcga gctcgacgaa
     6181 ctgcacgctc ttgcccgcgt cgcgcaacgc gctttccatc gtgcggctct gcacgatcgg
     6241 cacgaccgtg tcgtcgcggc cgtggatcag caacacgggc gctcgaacgc gctcggaatg
     6301 tcgtgcgggc gaggcggcgt ccaagtcctg cgatgacgac ccttcctcgt cgacgcccat
     6361 cgcctgccgc caataggaga ccgtcgcgct gcggccgccc gcacgctcgc gctcgaacgc
     6421 aagcatgtcg cgcaggtccg caactcccgc aatcgccgct gcgcagcggt agagctcggg
     6481 cgtgaacgcg gcgcccgcga gcgcagcata gccgccgtag ctcgcgccca cgatgcaaac
     6541 gcgctcggga tcggcgatgc cggctgcgat gagcgccgcg acgccgtcgg tcaggtcgtg
     6601 ctgcatgacg ccgatccccc attcgccgtg gccggcttcc tcccactcct ggccgtaacc
     6661 gcccgacccg cgaaaattcg gttgcaggac cgcgtaaccc cgagacgcca gcccatgagc
     6721 caaccagtcg tagccgctga agtcgcgcgc cgccgggccg ccgtgcggca gcaagaccaa
     6781 cggcgccggg ccttcgacac ctagcgggcg agtaaggtag gcgggaatct tgataccgtc
     6841 gcgcgccgca tagccgtagg cctcacggcg cgcgagcttg acggtcgcga gctgcatgtt
     6901 cgtgctcgcg atctgcttcg ccgtccccgc cttcgaatcc aagaagtagt aggcggcggc
     6961 acggtccgca ctttcggtgc tgacgatgga gcgcgagcgg tcttgcgacc acgaaacgat
     7021 gcgcggcgat tcgccgcgaa acgcttgatc gagctccgcc tgctgcgcgc gcagtgcgac
     7081 atcgaaccag acgatctctt ccggcccgac gtcggcgcca acgacgcgat tcgtgtaggg
     7141 gtcgacgcgc acgctctcga cgtcgaggct tgcgtgcgtg tacagcgggc gctcgatcgc
     7201 gcccgtctcg cccgacacga cgtaaaggcc gaatcggccg ccgttatcgc ttgct
//
LOCUS       DATLEE010000733         8623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6474157, whole genome shotgun sequence.
ACCESSION   DATLEE010000733 DATLEE010000000
VERSION     DATLEE010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6474157"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VM692_16080"
     CDS             <1..372
                     /locus_tag="VM692_16080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUQ53748.1"
                     /translation="RGHLESLKRDFTVAENLKFHAALWGQRLELGAILTELKLEKAAH
                     MRARYLSAGQRRRVALAMLRLSGAKLWLLDEPTTNLDSEGRTLVAAWTRQHVESGGIA
                     VVATHQPDEFAMRGALMIELQ"
     gene            369..1034
                     /gene="ccmB"
                     /locus_tag="VM692_16085"
     CDS             369..1034
                     /gene="ccmB"
                     /locus_tag="VM692_16085"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HUQ53749.1"
                     /translation="MKAFNAVFVRDLRVAFRRWSELAYPLIFFLIVVALFPLALSSTT
                     QQLREVGTGVLWVAALLSSLLALDGLFRGDADDGTLEQLLLSPVPMTITVLAKIAAHA
                     LVTLLPLICLVPATAWSYNMPPVALPTLALALLLAAPTLSVLIALGAALTVSLRRGGS
                     IVGLLVLPLTGPLLIFGTRATDLSVNGEPPTAPLLFLAALAVLAVSLGPLAIAAAVKV
                     GLE"
     gene            1047..1799
                     /gene="ccmC"
                     /locus_tag="VM692_16090"
     CDS             1047..1799
                     /gene="ccmC"
                     /locus_tag="VM692_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HUQ53750.1"
                     /translation="MKLPRWFHQLGSPPYVYRLAERLSPWLGGGAALLLAVGAYWGLV
                     IAPPDYQQGDSYRIIYIHPQTAYVGMMAYTVMAVAAGSGYVWRLKLAHAVAVAVAPIG
                     AWFTFCALFTGAVWGRPTWGTYWQWGDPRIMFEALLLFMFLGYLALRGAFDDRDKADR
                     VTAILAVVGVVNIPIIHYSVEWWNSLHQTSTILREGGPGMTTDMLIPFLIMVGGFTLM
                     FYWLLLRRLQGEIVEREGDARWIRELVAGSAP"
     gene            1814..1987
                     /gene="ccmD"
                     /locus_tag="VM692_16095"
     CDS             1814..1987
                     /gene="ccmD"
                     /locus_tag="VM692_16095"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HUQ53751.1"
                     /translation="MGGHGAYVWSAYGITLAVLLINAWLARAKRRAALRRAAELATQA
                     EVPRKQPTVRRLQ"
     gene            1984..2418
                     /gene="ccmE"
                     /locus_tag="VM692_16100"
     CDS             1984..2418
                     /gene="ccmE"
                     /locus_tag="VM692_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HUQ53752.1"
                     /translation="MKSRTQRKLFMVVVLAGITLAGFLAYRALQENLLYFFSPSQVAA
                     GEAPDRTFRLGGMVVAGSLKREVGTLNIRFVVTDNVHSVPVRYNRVLPDLFREGAGAV
                     VVGRLDSNGEFIGDEVIAKHDENYMPPEVAEALEKTRAAQGQ"
     gene            2415..4376
                     /locus_tag="VM692_16105"
     CDS             2415..4376
                     /locus_tag="VM692_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HUQ53753.1"
                     /translation="MIPEFGQLALALALCLALVQCSLPLLGAALGRQDWMALARPAAA
                     GQFVFVAIAFGTLEYAFLHDDFSVLFVAAHSNSALPVFYKATALWGGHEGSLLLWIAV
                     LAVWTMAVAAGSRSQPQEFASRVLGVLGIVSAGMIAFALITSNPFTRLLPAAADGNDL
                     NPLLQDPGMIFHPPTLYLGYVGVSVPFAFAVAALLTGRLDKDWAKWTRPWTVVAWIFL
                     TCGITLGSWWAYYELGWGGWWFWDPVENSSFMPWLMTTALLHSLAVTERRGIFKNWTV
                     LLAIAAFSLSLLGTFLTRSPVMISVHAFASDPTRGLFILGLLALFSGGALLLYALRAR
                     NLEAEGGFKLISRESFLMANNVLLVVATICILWGTLQPLFLDALNLGKISVGTPWFNM
                     MFLLPALPLVALIGVGMHSGWRMMPGDALWRRLRLPALAALIVGVAAPFIVFGTASVL
                     ALLSVCIAVWVCVSSMLDPFRRMIFRTGAPLTRGQVGMCLAHFGVGIFMLGATVASAY
                     NFERDVSARPGDRIEAGGYEFVFKSMRKVEGPNFVADEGEFELRSDGDLVTTLRPQIR
                     VYQVQAAPMTEAAIDTNVLRDVFLALGEPLGDNAWSLRLQVKPLIGFLWLGSGLMALG
                     GLVAITDRRYRMAVRQAERAAAQPAPGAA"
     gene            4378..4938
                     /locus_tag="VM692_16110"
     CDS             4378..4938
                     /locus_tag="VM692_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HUQ53754.1"
                     /translation="MWRYLVPLAIFVVIGVFFAVGLRLNPNYVPSPLVGKPIPTFSLP
                     TVTDADASLSSEMLKGRVSLVNVWGTWCIECGHEHPFLVQLARAGMPIYGLNVKDERA
                     PAVDWLNRLGNPYVSSGFDPEGIAAIDWGVTGAPETFLIGSNGTILHKHISPLTLRVW
                     QSDFLPLLREHCGPNDCPFLAAVGGQ"
     gene            4947..5354
                     /locus_tag="VM692_16115"
     CDS             4947..5354
                     /locus_tag="VM692_16115"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HUQ53755.1"
                     /translation="MWLFALWLGLAGSAFAIDEEVAFDDPLLNARYHALIREIRCPKC
                     LNESIAESKAPVAADLRREVRRLIGEGASDDEVKTFLSDRYGEFVLYRPRLSPTTLAL
                     WAGPFVFLALGGFIFWRILAGRRGQPLDEDPSL"
     gene            5351..6352
                     /locus_tag="VM692_16120"
     CDS             5351..6352
                     /locus_tag="VM692_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53756.1"
                     /translation="MTQTFWIVAALLCALAVAVLVVPIWRYKARAGAWSPAGLVAAAL
                     VVPMALALYSRVSNWDPAYAARADRENELLGQLVAHLDSNPNDVQGWRLLAASFMQLG
                     RYEEGRAAYQRLWALTREPDDELKLAYAEAQILTDRASLTGEAGKLVEEVLAARPADP
                     KALWYGGLVALELGRGDDVRTRWTSLLAMNPPPEVERVVRDQLASLEGVNADEAAVVA
                     SGPEIKLSVTLGAGRSLDSLGPNAQLFIMAVAPGGGPPLAVIRRPPSAVPGEFSLSDA
                     NAMLQGRSLATFDQVQVVARLSMSGQPIAQPGDWQAEAVVRPGEGATVALVIDQVVQ"
     gene            6357..7253
                     /locus_tag="VM692_16125"
     CDS             6357..7253
                     /locus_tag="VM692_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HUQ53757.1"
                     /translation="MPELPDITLYIEALRERILGRRLERSRVLSLFLLRSVEPSIDAV
                     TGATVVAFERLGKRIAIGFDNDIWLVLHLMIAGRLQWRADRPASFTRQTALALLFENG
                     TLVLTEAGTKKRASLYVVRGRAGLAAHQPGGVELERLRAAEFEAVLKSHNHTLKRALT
                     DPRLLSGIGNAYSDEILHRARLSPVTLTSRLTSEEYARLFAASRAVLAEWLERLRVKA
                     RGEFPSKVTAFHAEMAVHGRYGLPCPVCGTKVQRIRYADNETNYCPRCQTNGRLLADR
                     SLSRLLKKDWPKSIDELEQLRS"
     gene            7250..8500
                     /gene="dinB"
                     /locus_tag="VM692_16130"
     CDS             7250..8500
                     /gene="dinB"
                     /locus_tag="VM692_16130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HUQ53758.1"
                     /translation="MSVRSDTWPRIIVHADMDAFYAAIEQLDDPRLRGRPLLVGSDSA
                     RGVVLTASYEARSSGAGSAMPMQRARRLCPDALVVPPRFERYQEISETVMATLGDFSP
                     LVEPLSLDEAFLDMTGSTRLFGDPASIGARIKAAVRERTGGLTVSVGISATRYVAKVA
                     SGYRKPDALTIVPAQEMREWLAPMSVANLWGAGPKTAERLRSLGFETIGQVASTEPAR
                     LERMLGTLGRRFFSLANGVDPREVVGSRRAGSVGSERTLNVDVAARADIEAHLRLAAD
                     TIAHRLRRSQRRAHGVRVKLKTTDFRLLTRQCLLGEATDVAAVLFRQAAILLGDFADS
                     GPFRLVGLAAYELVAADDAGSQLDLLPAAGNRARRLETTIDALVGRFGAGVVQRAADL
                     ERDRGVGIAANLDFVHEHQREGED"
BASE COUNT         1263 a         2694 c         2927 g         1739 t
ORIGIN      
        1 cgcggccatc tcgaatcgtt gaagcgcgac ttcactgtcg ccgagaacct caagtttcac
       61 gccgcacttt ggggccagcg actcgagctc ggcgcgattc tcacggaact gaagttggaa
      121 aaggccgctc atatgcgggc gcgttacttg tcggcgggtc agcgcaggcg cgtcgcgctg
      181 gcgatgctgc gattgtcggg tgcgaagctg tggctgttgg atgagccgac gacgaatctc
      241 gatagtgaag ggcgaacatt ggttgctgct tggacgcgcc agcatgtcga aagcgggggc
      301 atcgccgtcg tcgcgactca tcagcccgac gagtttgcga tgcgcggcgc gctgatgatc
      361 gaactgcaat gaaagctttc aatgccgtgt tcgttcgtga tctgcgcgtg gcctttcggc
      421 gctggagcga gctcgcgtat ccgctgatct tctttctgat cgtcgtggcg ctgttcccgt
      481 tggcactgtc gtcgacgacg cagcaactgc gcgaagtcgg gaccggcgtg ttgtgggtag
      541 cggcgctgct gtcctctttg ctcgcgctgg acggcctttt ccgaggcgac gcggacgatg
      601 gcactctcga gcagctgctg ttgagccccg tgcccatgac gatcacggtt ctcgcgaaga
      661 tcgccgcgca cgcgctcgtg acgctgctgc cgctgatctg tcttgtccct gcgacggctt
      721 ggtcgtacaa catgccgccc gtagcattgc cgacattggc tctggcgttg ctgcttgccg
      781 cgccgacgtt gagcgttctc attgcattgg gtgctgcgct gacggtcagc ttgcgccgcg
      841 gcggctcgat tgtcgggtta ctggtgctgc cgttgacggg cccgctgttg attttcggca
      901 cgcgcgcgac agacctcagc gtcaacggcg agccgccaac ggcaccgctg ttgtttctcg
      961 ccgcgctcgc cgtgctggcg gtgtcgctcg gaccgctcgc aattgccgca gccgtgaagg
     1021 tagggctcga atagggaccc gtgaaaatga agttaccgcg ttggttccat caacttggtt
     1081 cgccgccgta tgtctatcga ctcgcggagc gattgagccc gtggctcggc ggaggcgccg
     1141 cgctactgct ggcggtcggc gcctattggg ggctcgtaat cgcgccgccc gactatcagc
     1201 agggcgactc gtatcgaatc atttatatcc atccgcagac cgcgtacgtc ggaatgatgg
     1261 cgtacacggt gatggccgtc gcagcaggga gcggctatgt gtggcgcctc aagctcgcgc
     1321 acgccgtggc cgtcgcggtc gcgccgatcg gcgcttggtt caccttttgc gcactgttca
     1381 cgggtgcggt ttggggtcgg ccgacctggg gaacttactg gcagtggggc gatccacgca
     1441 tcatgttcga ggccctgctg ctgtttatgt tcctcggcta tctcgcgcta cgcggggctt
     1501 tcgacgatcg cgacaaggcg gatcgcgtga cggcgattct cgccgtcgtt ggcgtcgtca
     1561 acatccctat cattcactac tcggtcgagt ggtggaactc gctccaccag acgtcgacga
     1621 tcttgcgcga gggcgggccg ggcatgacga cggacatgct gattccattc ctgatcatgg
     1681 tcggcggttt cacgctcatg ttctattggc ttttgttgcg gcggctgcag ggggagatcg
     1741 tcgaacgcga gggcgatgcg cgctggatcc gcgagctcgt ggcgggcagc gcgccgtgag
     1801 tgagtttttc caaatgggcg gccatggcgc ttacgtttgg tcggcctacg gcattacact
     1861 cgctgtattg ctcatcaatg cgtggctcgc gcgcgccaag cgccgagcgg cattgcggcg
     1921 agccgcagag ctggccacgc aggcggaagt tccgcgcaag caacctacgg tgcggcggct
     1981 gcaatgaaat cgagaacgca acgcaagcta ttcatggtcg tggtcctggc gggcatcacg
     2041 ctcgcgggat tcctggcgta ccgtgcccta caggaaaatc tcctgtactt cttcagtccc
     2101 tcgcaggtcg ctgcgggtga agccccggat cgtacgttcc ggctcggcgg catggtggtc
     2161 gccggcagcc tgaagcgtga ggtcggcacg ctgaacatca ggttcgtcgt cacggacaac
     2221 gttcactcgg tgccggtacg gtacaaccgc gtgctgccgg atctattccg cgaaggcgcg
     2281 ggcgctgtcg tcgtcggtcg gctcgacagc aacggcgagt tcatcggcga cgaagtcatc
     2341 gcgaagcacg acgagaacta catgccgccc gaggtcgccg aagcgctcga aaaaacgcgc
     2401 gcggcgcagg gccaatgatt ccggagtttg gtcagctcgc tcttgcgctg gctttgtgct
     2461 tggcgctcgt gcagtgcagc ttgccgctgc tcggtgccgc gctcgggcgc caggattgga
     2521 tggcgttggc gcggccggcc gcagccggcc agttcgtgtt cgtcgcaatc gcgttcggca
     2581 cgctcgagta cgcgttcctg cacgacgact tcagcgtgct gttcgtcgcg gcgcattcga
     2641 attcggcgct accggtcttc tacaaggcga cggcgctttg gggcggacac gaaggctcgc
     2701 tgcttttgtg gattgccgtg ctcgcggtct ggacgatggc cgtcgcggcg ggcagccgct
     2761 cgcagccgca agagttcgcg agccgcgttc tcggtgttct aggcatcgtc agcgccggca
     2821 tgattgcttt tgcgttgatc acctcgaacc cgttcacgcg attgcttccc gccgccgccg
     2881 acggcaacga cttgaatccg ctgctgcaag acccagggat gatctttcat ccgccgacgc
     2941 tgtatctcgg ttacgttggc gtgtccgtgc cgttcgcgtt cgccgtcgcc gcgctgctga
     3001 ccggccggct cgacaaggat tgggcgaagt ggacccggcc gtggaccgtc gtggcttgga
     3061 ttttcttgac ctgcggcatc acgctcggca gctggtgggc gtactacgag ctcggctggg
     3121 gtgggtggtg gttctgggac cccgtcgaaa actcgtcgtt catgccgtgg ttgatgacga
     3181 cggcgctgct gcactcgttg gccgtgacgg aacggcgcgg catcttcaag aactggacgg
     3241 tcctgctcgc gatcgccgcg ttctcgctga gcttgctcgg cacgttcctg acgcgctcgc
     3301 cggtcatgat ctcggtccac gcgttcgcct ccgatccgac gcgcggcctg ttcattctcg
     3361 gcttgctcgc gctgttctcc ggcggcgcgc tgctgctgta cgcattgcgc gcccgcaatc
     3421 tcgaagccga gggcgggttc aagctcatct cgcgcgaaag ctttctcatg gcgaacaacg
     3481 tgctgctcgt cgtcgccacg atttgcattc tgtggggcac gctgcagccg ctgttcctcg
     3541 atgcgctgaa cctcggcaaa atctccgtcg gcacgccgtg gttcaacatg atgttcctgt
     3601 tgccggcgtt gccgctcgtc gcgctgatcg gcgttggaat gcactcgggc tggcgcatga
     3661 tgccgggcga cgcgttatgg cggcgcctga ggttacccgc gctcgccgcg ctgatcgtcg
     3721 gtgtcgcggc gccgttcatc gtgttcggga cggcgtccgt tctggcgctg ctgtccgtct
     3781 gtatcgcggt gtgggtctgt gtcagctcga tgttggatcc gttccgcaga atgatcttcc
     3841 gcaccggcgc gccattgacg cgcggccaag tcggcatgtg cctcgcgcac ttcggtgtcg
     3901 gcattttcat gttgggtgcg acggtcgcgt ccgcgtacaa cttcgaacgc gacgtcagcg
     3961 cgcgacccgg cgatcgaatc gaagccggcg gctatgagtt cgtcttcaag agcatgcgca
     4021 aggtcgaagg cccgaatttc gtggccgacg aaggcgagtt cgagctccgc tccgatggcg
     4081 acctcgtgac gactctgcgg ccgcagatac gcgtgtacca agtgcaggcg gcgccaatga
     4141 cggaagcggc gatcgacacc aacgtgctgc gcgacgtgtt cctggccttg ggcgagccgc
     4201 tcggcgacaa cgcgtggagt cttcgcttgc aggtgaagcc gctgatcggt ttcttgtggc
     4261 tcggctccgg gctcatggcg ctcggcgggc tcgttgcgat cacggatcgg cgttatcgca
     4321 tggcggtaag gcaagcggaa agggccgcgg ctcagccggc gccgggagcg gcttgagatg
     4381 tggcgctatc tcgtgccgct cgcaatcttc gtcgtcatcg gcgtgttctt cgccgtcggt
     4441 cttaggctca atcccaacta cgtgccgtca ccgctcgtcg gcaagcctat tccgacgttc
     4501 tcgctgccga cggtcaccga cgccgatgcc tcgttgagct cggagatgct caagggccgc
     4561 gtttcgctcg tcaacgtctg ggggacgtgg tgtatcgaat gcgggcatga gcatccgttt
     4621 ctcgtgcagc tcgcgcgcgc cggcatgccg atttacgggc tcaacgtcaa agacgagcgg
     4681 gctccggccg tcgactggct caaccggctc ggcaatccct atgtcagctc cgggttcgac
     4741 cccgaaggca tcgccgcgat cgattggggg gtcacgggcg cgcccgagac tttcttgatc
     4801 ggcagcaacg ggacgattct gcacaagcac atctcgccgc tgacgctgcg cgtctggcag
     4861 agcgattttc tgccgctgtt gcgcgagcac tgcggtccga acgactgccc gtttcttgcc
     4921 gcggtcggcg gacaatgagg cggctcatgt ggctcttcgc gctgtggctc gggctcgcgg
     4981 gcagcgcctt cgcgatcgat gaagaagtcg ctttcgacga tccgctattg aacgcccgtt
     5041 atcacgcgct gattcgcgag atccgctgtc cgaagtgcct gaacgaaagc atcgccgagt
     5101 cgaaggcacc tgtcgccgcc gacttgcggc gcgaagtgcg gcggctcatc ggcgaaggcg
     5161 cgtcggacga cgaggtcaag acgttcttgt ctgaccgtta cggcgagttc gtgctgtatc
     5221 gaccgcgctt gtcgccgacg acattggccc tttgggccgg cccgttcgtg ttcttggcgt
     5281 tgggcgggtt cattttctgg cgaattctcg ctgggcggcg cggccagccc ctcgatgagg
     5341 atccgtcgtt gtgacgcaaa cgttttggat cgtcgccgcg ttgctatgcg cgctcgcggt
     5401 ggctgtcctc gtcgtgccga tctggcgcta caaggcgaga gcaggggcgt ggtcacctgc
     5461 gggcctcgtc gccgccgcac tcgttgtacc catggccctt gccttgtatt cgcgcgttag
     5521 caattgggac cccgcgtacg ccgcgcgcgc ggaccgagag aacgagcttc ttggccagct
     5581 cgtcgcgcac ctcgacagca atccgaacga cgttcaaggt tggcgcttgc tggcggcgag
     5641 cttcatgcag cttggacggt acgaggaagg gcgtgcggcg tatcagcgcc tttgggcgtt
     5701 gacgcgcgag cccgatgacg agctgaagct cgcctatgcg gaggcgcaga ttctgaccga
     5761 ccgcgcgagc ctgacgggcg aggccggcaa gctcgtcgag gaagtgctcg cggcacggcc
     5821 cgccgatcca aaggcgctct ggtacggcgg cctcgttgcg ctcgagctcg gccgcggcga
     5881 tgacgttcgg acgcgttgga cgagtctgct cgcgatgaat ccgccgcccg aggtggagcg
     5941 cgtcgtgcgc gaccagcttg cgagcctaga gggcgtgaac gcagacgagg cggccgtcgt
     6001 cgcgagcggc cctgaaatca agctcagcgt cacgctgggt gccgggcgct cgctcgattc
     6061 gctcgggccc aatgcgcaac ttttcatcat ggccgtggca cccggtggcg ggccgccgct
     6121 cgccgtgatt cgccggccgc cgagcgcggt gccgggcgag ttttcgttga gcgacgcgaa
     6181 cgcgatgctt caaggccgtt cgctagcgac gttcgatcaa gttcaggtcg tcgcacgctt
     6241 gtcgatgtcc gggcaaccca tcgcgcaacc cggcgactgg caggccgagg ccgtcgttcg
     6301 ccccggcgag ggggcaaccg tagcgctcgt catcgatcag gtggtccaat gagcgcatgc
     6361 ccgagttacc cgacatcacg ctctatatcg aagcgttgcg ggagcgaatt ctcggccggc
     6421 ggctagagcg cagccgcgtt ctttccctgt ttctgttgcg cagcgtcgag ccgtcgatcg
     6481 acgctgtgac gggcgcgacg gtcgtcgcct tcgagcgtct cggtaagcgc atcgcgatcg
     6541 ggttcgacaa cgatatctgg ctcgtgctgc atctcatgat cgccgggcgc ttgcagtggc
     6601 gcgcggaccg gcccgcttcg ttcacgcggc aaacggcgtt ggcgctcctg ttcgagaacg
     6661 gcacgctggt gctgacagaa gccggcacga agaagcgcgc gtccctgtac gtcgtgcgcg
     6721 ggcgtgcggg tcttgccgct catcaacccg gcggtgtgga gctcgaacgt ctcagggcgg
     6781 cggagttcga agccgtgctc aagagccaca accacacgtt gaagcgtgcg ttgacggacc
     6841 cgcgtctgct cagtggcatc ggcaacgcat actcggacga gattctgcat cgcgcgcgct
     6901 tgtcgccggt cacgctgacg agtcgactga cgtcggagga gtacgctcgg ttgttcgcgg
     6961 cttcgcgcgc ggtgttggct gaatggttgg agcgcctgcg cgtgaaagcg cgcggcgagt
     7021 ttccgagcaa ggtcacggcg tttcatgccg agatggccgt gcacgggcgc tacgggttgc
     7081 cgtgccccgt ctgcggcacg aaagtccagc gcatccgcta tgccgacaac gagaccaatt
     7141 attgcccgcg ttgccagacc aacggccgct tgttggccga ccgatccttg tcgcgcctct
     7201 tgaagaagga ttggccgaag tcgatagacg agctcgagca gctgcgatca tgagcgtccg
     7261 aagcgacacc tggccgcgca tcatcgtgca cgccgacatg gacgcgttct atgcggccat
     7321 cgagcagctc gacgatccgc gactgcgtgg ccgcccgtta ctcgtgggct ccgacagcgc
     7381 gcgcggcgtc gtgttgacgg ccagctatga ggcgcgttcc tcgggcgcgg gaagcgcgat
     7441 gccgatgcag cgcgcgcggc gtctttgccc cgacgcgctc gtcgtgccgc cgcgcttcga
     7501 gcgctatcaa gaaatttcgg aaaccgtcat ggcgacgctc ggcgatttct caccgctcgt
     7561 ggagccgctg agcctcgacg aagcctttct cgacatgacg ggcagcacgc ggttgttcgg
     7621 cgaccctgcg tcgatagggg cacgcatcaa ggcggcggtg cgtgagcgca cgggcgggct
     7681 cacggtttcc gtcggcatct cggcgacgcg ctacgtcgcc aaggtcgcga gcggctatcg
     7741 caagccggac gcgctcacga tcgtgccggc gcaagagatg cgcgaatggc tggcaccgat
     7801 gtcggtggcg aacctctggg gcgccgggcc gaagacggcc gaacggctgc gctcgctggg
     7861 cttcgagacg atagggcagg tcgcaagcac cgagcctgcg cgcctggagc gaatgctcgg
     7921 aactctcggc cgtcggtttt tttcgctcgc caacggcgtc gatcctcgcg aggtcgtcgg
     7981 ctctcgccgt gccggtagcg tcggctccga gcgcacgctg aacgtcgacg tcgcggcgcg
     8041 ggccgatatc gaggcacatc tgcggcttgc ggcggacacc atcgcgcatc gcttgcggcg
     8101 ctcgcagcgg cgggcgcacg gcgtgcgcgt caagctcaag acgaccgatt ttcgcctgtt
     8161 gacacgccaa tgcttgctcg gcgaagcgac ggatgtggca gccgtgttgt tccggcaagc
     8221 ggcgattttg ctcggcgact tcgccgactc cgggccgttc cggctcgtcg gcctcgctgc
     8281 ttacgagctc gttgcggcgg acgacgcggg ttctcagctc gatctgctgc ccgcggcggg
     8341 caatcgcgcg cggcgcctcg agacgacgat cgacgcgctc gtcggccgct tcggtgccgg
     8401 ggttgtgcag cgcgcagccg acctcgagcg cgatcgaggc gtcggcatcg cggccaactt
     8461 ggatttcgtg cacgagcatc agcgagaagg cgaggactag ctcgcgtcgc cggccgcgac
     8521 ggcggcggct tgctcctcga gctcggccca ccgatcgacg gccgtttcga cttcctgctc
     8581 ggcgctgcgg agctcggcga gggcttgttg tacgtcttga tgc
//
LOCUS       DATLEE010000734         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3667409, whole genome shotgun sequence.
ACCESSION   DATLEE010000734 DATLEE010000000
VERSION     DATLEE010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3667409"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1390)
                     /locus_tag="VM692_16135"
     CDS             complement(128..1390)
                     /locus_tag="VM692_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HUQ53759.1"
                     /translation="MKKNVFYAQSGGVTAVINASACGVIETARKHKNKLGRVYAGRNG
                     ILGALTEDMIDTTAESARTIAALRHTPGGAFGSARYKLKGLDKNRAEYERLIEVFRAH
                     DIGYFFYNGGGDSMDTAHKVSQLGEELGYPIVCIGVPKTVDNDLPVTDTCPGFGSVAK
                     YVAVSTREAALDVASMARTSTRVFVLEVMGRHAGWIAAAGGLAGEGPADAPHVILFPE
                     IPFDEAAFLARVKDSVEKYEYCVVVVSEGTRYADGRFLAEAGGKDAFGHAQLGGVAAT
                     VAQLVKAKLGFKFHYAIADYLQRAARHIASKVDVEQAYAMGKAAVELALQGENAVMPT
                     IVRKSDRPYRWGVGKVALAEVANKEKMMPRNFITEDGFGITAACRRYLAPLIRGEDYP
                     PYKDGLPTYVSLKNQRVAKKLKGEFVIK"
     gene            1512..2159
                     /locus_tag="VM692_16140"
     CDS             1512..2159
                     /locus_tag="VM692_16140"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HUQ53760.1"
                     /translation="MRIVMLGAPGSGKGTQAQRIQKDHGLPQVSTGDLLRRAVADKTP
                     LGLKAKKAMDAGELVSDEIVLGMIKERIAQPDAKKGFILDGFPRNTSQANSLDVLLGE
                     LKLTLDRVVLMDVDFDILMKRLTGRRTCSKTGAVLNIYFSSPAELEACRKAGGELVQR
                     DDDNEATIRNRLQVYERQTAPLVDYYTKKGLLKTVAATGEVDAVYSRLKSALGLK"
BASE COUNT          389 a          747 c          742 g          392 t
ORIGIN      
        1 cctaaaagac ctttgaaatc aacccagtga agctctccgt ctaggaaggg gcgtcggcgt
       61 caagcgagac gggtttgttc cctgttgttc atccatgctg cgccggcccc ccccggcgaa
      121 gcgggcgcta ctttatcaca aactcgcctt tgagcttttt cgcaacccgc tgattcttga
      181 ggctgacgta agtgggcagg ccgtccttgt agggcgggta gtcctcgccg cggatcagcg
      241 gggccagata ccggcggcaa gcggccgtga taccgaaccc gtcctcggtg atgaaattcc
      301 gcggcatcat cttctctttg ttggctacct cggccagcgc gaccttgccg acgccccacc
      361 gatacggccg gtcggactta cggacgatcg tcggcatgac ggcgttctcg ccttgcaacg
      421 cgagctcgac ggcggccttg cccatggcgt aggcctgctc gacatcgacc ttcgaggcga
      481 tgtggcgcgc cgcgcgctgc agatagtccg cgatcgcgta gtggaacttg aacccgagct
      541 tggctttgac gagctgcgcg acggtagccg cgacgccgcc gagctgcgca tggccgaacg
      601 cgtccttgcc gccggcctcg gctaagaagc ggccgtcggc atagcgggtg ccctcggaaa
      661 cgacgacgac gcagtactcg tacttctcga cgctgtcctt gacgcgcgcg aggaacgcgg
      721 cctcgtcgaa cggaatctcg ggaaacagga tcacgtgcgg cgcgtcggcc gggccttcgc
      781 cggcgaggcc gccggccgcg gcgatccagc cggcgtggcg gcccatgacc tcgagcacga
      841 acacgcgggt cgacgtgcgg gccatcgatg cgacgtcgag cgcagcctcg cgcgtcgaga
      901 cggccacgta cttcgcgacc gagccgaacc cggggcacgt gtccgtgaca ggcaggtcgt
      961 tgtcgacggt cttcggcacg ccgatgcaga cgatcgggta gccgagctcc tcgccgagct
     1021 gcgacacctt gtgggcggta tccatcgaat cgccgccgcc gttgtaaaag aaatagccga
     1081 tgtcgtgggc cctgaagacc tcgatgagcc gctcgtactc ggcgcggttc ttgtcgagcc
     1141 ctttgagctt gtagcgcgcg gacccgaagg cgccgcccgg tgtgtgccgc agcgcggcaa
     1201 tggtgcgcgc ggactcggcc gtcgtgtcga tcatgtcttc ggtcagcgcg ccgagaatcc
     1261 cgttgcgccc ggcgtagacg cggccgagct tgttcttgtg cttgcgcgcc gtctcgatga
     1321 cgccgcaagc gctcgcattg atgaccgcgg tcacgccccc cgactgggcg taaaaaacat
     1381 tcttcttcac gggtcctccc ggactttgca cgacttgcct ccgagaatag caaagcggct
     1441 gcgcgtttga cgaaggtcgc gcgcgtcgct agcgtagcga ccgtttcgag tctgtccgtg
     1501 ggagatccgt catgcgaatc gtcatgctcg gtgcgccggg ttcgggcaag ggcactcagg
     1561 cgcagcgcat ccaaaaggac cacggactgc cgcaagtttc gacgggcgac ctgctgcgcc
     1621 gcgccgtcgc cgacaagacg ccgctcgggc tcaaagcgaa gaaggccatg gatgccggcg
     1681 agctcgtgtc tgacgagatc gtgctcggca tgatcaagga gcgcatcgcg cagcccgatg
     1741 cgaagaaggg cttcatcctc gacggcttcc cgcgcaacac gagtcaagcg aacagcctcg
     1801 acgtgttgct cggtgagctc aagctgacgc tcgatcgcgt cgtgctgatg gacgtcgatt
     1861 tcgacatcct catgaagcgg ctgacggggc gccgcacgtg ctcgaagacg ggcgccgtgc
     1921 tgaacattta tttctcgtcg cccgcggagc tcgaggcgtg ccgcaaagcc ggcggcgagc
     1981 tcgtgcagcg cgacgacgac aacgaggcga cgatccgcaa tcgcctgcag gtctacgagc
     2041 gccagaccgc accgctcgtc gactactaca ccaagaaagg cctgctgaag accgtggccg
     2101 ccacgggcga ggtcgacgcc gtctactcgc gcctgaaatc cgccctcggt ctcaaataga
     2161 cgctcatcgc agtcgccgca gcgagcgccg agcagggctt ccgagacacg ccggaagtgc
     2221 gtccctgcgg ctcgtcggcg cacgtccctg tgcgccgacg gtctcgggag
//
LOCUS       DATLEE010000735         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11097039, whole genome shotgun sequence.
ACCESSION   DATLEE010000735 DATLEE010000000
VERSION     DATLEE010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11097039"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VM692_16145"
     CDS             <1..316
                     /locus_tag="VM692_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53761.1"
                     /translation="DPAFVLVETALGADGEPVTTSEQAANRVVNGKTRWHVVTLGQSV
                     PLSHASALEGAVSFAASRNIPVVYERDESAALSQAAAPSSGLTGVAASAALAAASSSA
                     AK"
     gene            complement(232..1260)
                     /gene="dusB"
                     /locus_tag="VM692_16150"
     CDS             complement(232..1260)
                     /gene="dusB"
                     /locus_tag="VM692_16150"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HUQ53762.1"
                     /translation="MRIGPYSIDPPLILAPMAGVTDKPFRLLCKRLGAGYAVSEMTTA
                     DPRLWRTRKSAQRLDHEGEPAPIGVQIAGADPQALAAAAQHAVNHGAEIVDINMGCPA
                     KKVCRAWAGSALLQDEALVARIVTAVVNAVPVPVTLKIRTGWDRDHVNGVEVARIAEH
                     AGISALAVHGRTRDMLYTGEAEYETIAAIKQAVRIPVVANGDVRSPHKAREVLAATGA
                     DALMVGRAAQGRPWIFREIAHFLATGQLLPPPPIAEVSDTLLAHVRALHEFYGELAGV
                     RIARKHLAWYAKDRAENETFRAVVNRAETADEQLRLTRDYFAALEDAAASAALAATPV
                     RPELGAAA"
     gene            complement(1296..>2183)
                     /locus_tag="VM692_16155"
     CDS             complement(1296..>2183)
                     /locus_tag="VM692_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HUQ53763.1"
                     /translation="WVYEGLTQYYGYVLAARSSLWSVEQARDALALTAATYQARVGRE
                     WRSVADTTNDPILSARRPAPWRSWLRNEDYYSEGLLVWLDADTLIRERTDGRRSLDDF
                     SRAFFGSNDGSWVPVTYTFDDLVAALNAVYAHDWATFLHDRVDEAGTEPPLDGLTRGG
                     YRLVFREERSAFQRDAEAHNGNADFSYSVGLIFGDGGNVTGVQWDGPAFNQGVTVGTQ
                     VVAVNGVAFSNDALRRAITAAKTDAALELLLKTGDQYRTITLDYRGGLKYPQLERAAA
                     SSDRLSAILAPRTAAPRSR"
BASE COUNT          340 a          739 c          743 g          361 t
ORIGIN      
        1 cgatcccgcg ttcgtgctcg tcgagacggc gcttggcgcc gacggcgagc ccgtgacgac
       61 gagcgagcaa gccgccaaca gagtcgtcaa cggcaagacg cgttggcacg tcgtcacgct
      121 cgggcaatcg gtcccgctct cgcacgcgtc ggcgctcgaa ggggccgtgt cgttcgccgc
      181 aagccgcaac attcccgtcg tctacgagcg cgacgagtcg gctgcgctga gtcaggccgc
      241 ggcgccgagc tcgggcctga cgggcgtggc ggcgagcgcc gcgctcgcgg ccgcgtcttc
      301 gagtgccgcg aaatagtcgc gcgtcaaccg cagttgctcg tcggctgtct ccgcgcgatt
      361 gacgacggcg cgaaacgttt cgttctccgc acgatccttg gcgtaccagg ccaagtgctt
      421 gcgcgcgatg cgcacgccgg ccagctcgcc gtagaactcg tgcagggctc gtacgtgcgc
      481 gagcagagtg tcgcttactt cggcgattgg cggtggaggc aatagctggc ccgtcgcgag
      541 gaagtgcgcg atctcacgga agatccacgg ccggccctgc gccgcgcgac cgaccatcag
      601 cgcgtcggcg cccgtcgctg ccagcacctc gcgagctttg tgcggcgacc tcacgtcgcc
      661 gttggcgacg acagggatgc gcacggcttg cttgatcgcg gcgatcgtct cgtactcggc
      721 ctcgcccgtg tagagcatgt cgcgcgtgcg gccgtgcacg gcgagcgcgc tgatgccagc
      781 gtgctcggcg atgcgcgcga cttcgacgcc gttgacgtgg tcgcggtccc agcctgtgcg
      841 aatcttcaac gtcacgggca cgggcacggc attcacgacg gccgtcacga ttcgggcgac
      901 gagcgcttcg tcttgcagca gagccgagcc cgcccaagcg cggcagactt tcttcgcggg
      961 gcagcccatg ttgatgtcga cgatttcggc gccgtggttg acggcgtgct gcgcggcggc
     1021 tgcgagcgcc tgcggatcgg cgccggcgat ctgcacgccg atcggtgcag gctcaccttc
     1081 gtgatcgaga cgctgtgcgc tcttgcgtgt gcgccagagc cgcggatcgg ccgtcgtcat
     1141 ctccgagact gcatagccgg cgccaaggcg tttgcagagc aaacgaaacg gcttgtccgt
     1201 gacgccggcc atgggcgcca ggatcagcgg cggatcgatg ctgtaggggc cgatacgcat
     1261 agcgggacgc gagtctagca ctcgacgggc cggcgtcagc gtgaccgcgg cgccgccgtt
     1321 cggggcgcca gaatcgcgct caagcggtcg ctcgacgccg cagcgcgctc gagctgcgga
     1381 tacttgagcc caccgcgata gtcgagcgtt atcgttcgat attggtcgcc tgtcttcagc
     1441 agcagctcga gcgccgcatc ggtcttggcc gccgtgatcg ctctgcgcaa cgcgtcgttg
     1501 ctgaacgcga cgccgttcac ggccacgact tgcgtgccga cggtgacgcc ttgattgaac
     1561 gccgggccgt cccactggac gcccgtgacg ttgccgccgt cgccgaagat caagcctacc
     1621 gagtagctga aatcggcatt gccgttgtgt gcctcggcgt cgcgctggaa cgcgctgcgc
     1681 tcttccctga acacgagccg gtacccgccg cgcgtcaaac cgtcgagcgg gggctcggta
     1741 ccggcttcgt cgacgcggtc gtgcaggaac gtcgcccagt cgtgcgcata gacggcgttc
     1801 agcgccgcga cgagatcgtc gaacgtgtag gtgacgggca cccacgagcc gtcgttgctg
     1861 ccgaagaacg ccctcgagaa atcatccagc gagcgccggc cgtcggtgcg ctcgcgaatc
     1921 aacgtatcgg catcgagcca tacgagcagg ccctcggagt aatagtcttc gttgcgcaac
     1981 cagctgcgcc aaggcgccgg ccggcgcgcc gagagaatcg gatcgttcgt cgtgtcggcg
     2041 accgagcgcc actcgcgacc gacgcgcgct tgataggttg ccgccgtcag cgccaacgca
     2101 tcgcgcgcct gctcgacgga ccaaaggctc gaacgcgccg cgagcacata gccgtagtac
     2161 tgcgtcagcc cttcataaac cca
//
LOCUS       DATLEE010000736         2618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7377383, whole genome shotgun sequence.
ACCESSION   DATLEE010000736 DATLEE010000000
VERSION     DATLEE010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7377383"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /gene="purF"
                     /locus_tag="VM692_16160"
     CDS             complement(<1..1323)
                     /gene="purF"
                     /locus_tag="VM692_16160"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010794935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HUQ53764.1"
                     /translation="MCGLVGIVGHEPVNQSLYDALTVLQHRGQDAAGIMTDDGGRLRV
                     RKGNGLVRDVFLERHMLKLAGNVGLGHVRYPTAGSASLSEAQPFYVNSPYGICLAHNG
                     NLVNADELKRLVEQQDHRHLNTNSDSEVLLNVFAHELSKHTTNGLKPQQILDAVEALY
                     KRCSGGYAVVAMIIGYGVVAFRDPHGIRPLVLGIRETANGVEHMVASESVALDAVGFE
                     LERDILPGEAVFIDKRGYLHSRQTAARRGYTPCVFEFVYFARPDSIIDNLSVYKARLR
                     MGEKLAAKIMRVRPDHDIDVVIPIPDTSRTAAMQVAQGLGVKYREGFIKNRYIGRTFI
                     MPEQSARKASVRRKLNAIGLEFAGKNVLLVDDSIVRGTTSQQIIDMAREAGAKKVYFA
                     SAAPPVRYPNVYGIDMPAAGELVAAGRTPEQVQRKIGADWLIYQDLDDL"
     gene            complement(1354..1881)
                     /locus_tag="VM692_16165"
     CDS             complement(1354..1881)
                     /locus_tag="VM692_16165"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HUQ53765.1"
                     /translation="MTPVDYVILFLTLVSVLVGIWRGFTTEALSLVTLLAAIALAWTF
                     AGLLEPSLGNWASAVEVRLWTARVIIFVAVLVVGGVISWLARKLIRHTGLSGLDRTLG
                     AGFGLLRAAVLIGLAVIVLQFAELDQEAWWQEAQLRPYAERAAAAVKYYAELGTRYLQ
                     EQPAPQSASARVRRL"
     gene            complement(1882..2511)
                     /locus_tag="VM692_16170"
     CDS             complement(1882..2511)
                     /locus_tag="VM692_16170"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HUQ53766.1"
                     /translation="MDQGLKERLVGAAVLVAIAVWLIPWVLDGPEAPREPNGGSLQLP
                     AAEEPMPMRTQTLRLGDAAEPVVESPPPTAAAPPQDDPAPTAEPPAAPAAEAEAPVVA
                     AAQPEPQRAPVVATAPAPKPPPARSAAPPASGDWIVQLGSFGEEANARRVAQRSGTFG
                     YKVEVSTVKNGGRTLYRVRVGSARSRAEAEATVSALKAHGIDASVVAAR"
BASE COUNT          469 a          885 c          839 g          425 t
ORIGIN      
        1 gagatcgtcg aggtcttgat agatcagcca gtctgcgccg atcttgcgct gcacctgttc
       61 cggcgtgcgg cccgccgcga cgagctcgcc ggcggccggc atatcgatgc cgtagacgtt
      121 cggataacgc accggcggag ccgccgaggc gaaatagact ttcttggcgc ccgcctcgcg
      181 tgccatatcg atgatttgct gcgaggtcgt gccgcgaacg atcgagtcgt cgacgagcag
      241 cacgttcttg ccggcgaact cgagcccaat cgcgttcagc ttgcgccgca ccgacgcctt
      301 gcgcgccgat tgctccggca tgatgaacgt gcgcccgatg taacgatttt tgatgaagcc
      361 ctcgcgatac ttgaccccga gcccctgagc gacctgcatc gcggcggtgc gcgacgtgtc
      421 ggggatcggg atgacgacgt cgatgtcgtg atccggccgc acgcgcatga tctttgctgc
      481 gagtttctcg cccatgcgca agcgggcttt gtagacggac aagttgtcga tgatcgagtc
      541 gggccgcgca aagtagacga actcgaagac acacggcgtg tagccgcgcc gcgcggccgt
      601 ctggcggctg tgcaagtagc cgcgcttgtc gatgaacacg gcttcgccgg gcagtatgtc
      661 gcgctcgagc tcgaacccca cggcgtcgag cgccacgctc tccgacgcca ccatgtgctc
      721 gacgccgttc gccgtctcgc gaatcccgag cacgagcggc cgaatgccgt gcgggtcgcg
      781 gaacgccacg acgccgtaac cgatgatcat cgcgacgacg gcatagccgc cggagcagcg
      841 cttatagagc gcctcgacgg catcgaggat ctgctgcggt ttcagaccgt tcgtggtgtg
      901 cttgctgagc tcgtgcgcga acacgttcag cagcacttcc gagtccgagt tcgtgttcag
      961 gtgccggtgg tcttgctgct cgacgagcct cttcagctcg tcggcattga cgaggttgcc
     1021 gttgtgcgcc aggcagatcc cgtacggcga attgacgtag aacggctgag cctccgacag
     1081 actggcactg ccggcggttg ggtaccggac atggccgagg ccgacgttgc cggcgagctt
     1141 caacatgtgg cgctcgagaa agacgtcgcg gacgaggccg ttgcccttgc ggacccggag
     1201 ccggccgcca tcgtccgtca tgatgccggc cgcgtcctga ccgcggtgct gcagcaccgt
     1261 cagcgcgtcg tagagcgact gattgacggg ctcatgaccg acgattccga ccaatccaca
     1321 catgggtgca cctacagagc tatagcgaag acactaaaga cgccggacgc gagccgaagc
     1381 agattgcggc gcaggttgct cttgcaaata ccgcgtgccg agctcggcat agtacttgac
     1441 cgcggcggcg gcgcgttccg catagggccg tagctgtgct tcctgccacc acgcctcctg
     1501 atcgagctcc gcgaactgca acacgatgac ggcgagcccg atgagcaccg cggctcgcaa
     1561 caagccgaat cccgcaccga gcgtccgatc gaggccgctc aggcccgtgt gccggatcaa
     1621 ttttctcgca agccaggaga tgacaccacc gacaacgaga accgcgacaa aaataatgac
     1681 tcgcgccgtc cacaacctga cctcgacggc cgaggcccag ttcccgaggc ttggctcgag
     1741 cagccccgcg aacgtccagg ccaaggcgat cgcggccagc aacgtcacga gcgacaaagc
     1801 ctcggtcgtg aaccctcgcc aaatgccaac gagcacggaa accagagtca gaaacaagat
     1861 gacgtaatcg accggcgtca ttcaacgcgc agcgacgaca ctggcgtcga tgccgtgagc
     1921 cttcaacgcc gacaccgtcg cctcggcctc ggcgcgcgag cgcgccgagc ctacgcgcac
     1981 tcgatacagc gtgcggccgc cgtttttgac cgtcgaaact tcgaccttgt acccgaacgt
     2041 gccgctacgc tgcgcgacgc ggcgggcatt ggcttcctcg ccgaagctgc cgagctgaac
     2101 gatccaatcc ccgctcgccg gtggggccgc ggaccgcgca ggcggcggct tcggcgccgg
     2161 cgccgtcgcg actacaggtg cgcgctgcgg ctcgggctgc gcagccgcaa ccacgggcgc
     2221 ctcggcctcg gccgccggtg ccgcgggcgg ctcggccgtc ggcgcagggt cgtcctgcgg
     2281 cggcgctgcg gccgtcggcg gcggcgattc gacgaccggc tctgccgcat cgcccaaacg
     2341 cagcgtctgc gtacgcatcg gcatcggctc ttcggccgca ggcagctgca gtgagccgcc
     2401 attcggctcg cgcggcgctt cgggaccgtc gagcacccaa gggatcaacc acactgcgat
     2461 tgcgaccagc acggccgcac cgacgagtcg ttccttcaaa ccctgatcca tgtggcgggt
     2521 cggtcaacca atggggaggg tgcgcagtat agccgaagcg tttccatcgc tgctcccacc
     2581 gtgagaaacg atccgtacac gacgacacga tcgccggc
//
LOCUS       DATLEE010000737         2668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11105409, whole genome shotgun sequence.
ACCESSION   DATLEE010000737 DATLEE010000000
VERSION     DATLEE010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11105409"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VM692_16175"
     CDS             <1..1235
                     /locus_tag="VM692_16175"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ53767.1"
                     /translation="TSGAIVVEGKVISGRTCNSARSNCYIAAHDARTGKEAWRFYTSP
                     DAGEPGNDSWGGTPVDERRAAAWGLPGTYDPVRRLVYWGIANPMPNTRADRHGGNVDG
                     TARHAPADLYSNSTVALKPDTGELVWHYQHLPGDDWDMDINEERVLVRTVVDPDPRHV
                     KWINPNVRKGVERDVAITVGEGGGMWLNDRATGEFLWAMPFPYDTDHWILEDIDVATG
                     VAHINSKVLLDAPGVTKLVCYWNTRSFWPTAYSPKTNSLYVPYIDHCLTMTRAGVNGE
                     PERRTSGLRPGADPAKLAGIAKIDLKTGEIHRIYEARMAGNGAMLATAGDLLFWGDIG
                     QVLRAFDADTGKILWESEPLGATVQTSTITYAVDGKQYVAVINAESLLGAQRLAAVAE
                     ATLPPRGGNSINVFALPR"
     gene            1303..>2668
                     /locus_tag="VM692_16180"
     CDS             1303..>2668
                     /locus_tag="VM692_16180"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HUQ53768.1"
                     /translation="MLTKDASIAANRFGLGARPDDARSSGNDPRGWLEAQLAAPRGGR
                     PPGEPQSARVLREVRELRAARQVAAQARANLTQPPAQGAAPPATPGIDEQAIREFGAF
                     ARERYLEQTAERHRLALTTDEPFVERLVHFWSNHFAVSADKQPLAAIAGLYEQEAIRP
                     HVTGNFYDLLLAAVRHPAMILYLDNQTSMGPSSTAANFVRRKRGRELGLNENLAREIL
                     ELHTLGVAGGYAQADVMEFAKVLTGWSIGGGLGQGGRVLERLGGDAGEPGEFHFRAGM
                     HEPGDKTILGKRYRDGGADEGQEVLRMLTLHDSTARHLATKLARHFVADEPPPKLVER
                     LADVYRRNDGELKPVYRALLAADESWREPLAKFKTPHDFALSAYRSLGFVPDNLQPIT
                     AFLTQAGQRPYAPGSPAGWPDTAASWNGGDALLKRIELATAAGRRTGDRIDPLARAGE
                     VLGEL"
BASE COUNT          470 a          928 c          895 g          375 t
ORIGIN      
        1 acacgtccgg cgcgatcgtc gtcgaaggca aagtcatctc aggccgcacg tgcaacagcg
       61 cgcgctcgaa ttgctacatc gcagcccacg acgcgcgcac gggcaaggag gcgtggcgct
      121 tctatacgag ccccgacgcc ggcgagcccg gtaacgattc gtggggcggc acacccgtcg
      181 acgagcggcg cgccgccgcg tggggcttgc cgggcactta cgatcccgtg cgccggctcg
      241 tctattgggg catcgccaac ccgatgccga acacgcgcgc cgaccggcac ggcggcaacg
      301 tcgacggaac ggcgcggcac gcgccggccg atctctacag caactcgacc gtggcgctga
      361 agcccgacac cggcgagctc gtgtggcact accagcactt gcccggcgac gactgggaca
      421 tggacatcaa cgaagagcgc gttctcgttc gcaccgtcgt cgatcccgat ccgcggcacg
      481 tcaagtggat caatccgaac gtgcggaaag gcgtcgagcg cgacgttgcg atcacggtcg
      541 gcgaaggcgg cggcatgtgg ctcaacgatc gcgccacggg cgagttcctg tgggcaatgc
      601 cgtttccata cgacaccgat cactggatcc tcgaggacat cgacgtcgcg accggcgtcg
      661 ctcacatcaa ctcgaaggtg ctgctcgatg cgcccggcgt cacgaagctc gtctgctact
      721 ggaacacgcg cagcttctgg ccgacggcgt actcgccgaa gacgaactcg ctgtacgtgc
      781 cgtacatcga ccactgcttg acgatgacgc gcgcgggagt gaacggcgag cccgagcgtc
      841 gcacgagcgg cctgcggccc ggggcggacc cggccaagct cgccggcatc gcgaagatcg
      901 atctcaagac cggcgagatc caccgtatct acgaagcgcg catggccggc aacggcgcga
      961 tgctggcgac ggccggcgac ctcttgttct ggggcgacat cggccaagtg ctgcgcgcct
     1021 ttgacgccga tacgggtaaa atcttgtggg agtcggagcc gctcggcgcg accgtccaga
     1081 cgagcacgat cacttacgcc gtcgacggca agcagtacgt agccgtcatc aacgctgaat
     1141 cgctgctcgg cgcgcagcgc ctggccgccg tcgcggaagc gacgctgcca ccgcgcggcg
     1201 gcaactcgat caatgtattc gcactgccgc gctaggcgtg taaaccgacc ggcccctagg
     1261 gccgtttagc ctggagaccg accaccgacg cgaggaacgt cgatgttgac taaagatgcc
     1321 agcatagccg ccaatcggtt cgggctcggc gcccgacccg acgacgcgcg ttcgagcggc
     1381 aacgatccgc ggggctggct cgaagcgcag ctcgcggcgc cgcgcggcgg ccggccaccc
     1441 ggcgagccac agtctgcacg tgtgctgcgt gaagtgcgcg agctgcgcgc ggcgaggcaa
     1501 gtagccgcgc aagcgcgcgc gaatctcacg caaccgcccg cgcagggggc cgctccgccc
     1561 gcgacacccg gcatcgacga gcaggcgatc cgcgaattcg gtgccttcgc tcgcgagcgt
     1621 tatctcgagc agaccgctga gcgccatcgg ctcgcgctga cgactgacga gccgttcgtc
     1681 gagcggctcg tgcatttctg gagcaatcac ttcgccgtgt cggcggacaa gcaaccgctg
     1741 gccgcaatcg ccggtctcta cgagcaggag gccattcggc cgcacgtcac gggcaatttc
     1801 tacgacttgc tgctcgcggc cgtgcgccat cccgcgatga tcctctatct cgacaaccag
     1861 acctccatgg ggccgagctc gaccgcggca aatttcgtgc ggcgcaaacg cggccgcgag
     1921 ctcgggctca acgagaacct cgcgcgcgag atcctggagc tgcacaccct cggcgtcgcc
     1981 ggcggctatg cgcaagccga cgtcatggaa ttcgcgaaag tgctgacggg ctggtcgatc
     2041 ggcggcgggc tcgggcaagg cggccgcgtg ctcgagcgcc tcggcggcga tgcaggcgaa
     2101 cccggcgaat tccattttcg cgccggcatg cacgagcccg gcgacaagac gatactcggc
     2161 aaacgctacc gcgatggcgg cgccgatgaa ggccaggaag tgctgcgcat gctcacgctg
     2221 cacgacagca cggcgcgtca tctcgcgacg aagctcgcgc ggcatttcgt cgccgacgag
     2281 cctccgccga agctcgtcga gcggttggcc gacgtctacc ggcgcaacga cggcgagctg
     2341 aagcccgtct accgcgcgct gctcgcggcc gacgagagct ggcgcgagcc gctcgcgaaa
     2401 ttcaagacgc cgcacgactt cgcgctgtcg gcgtatcgct cgctcggctt cgtgccggac
     2461 aacctgcagc ccatcacggc gtttctgacg caagccggcc agcggccgta cgcgccgggc
     2521 tcgccggccg gttggcccga cacggccgcg agctggaacg gcggcgacgc gctgctgaaa
     2581 cgcatcgagc tcgcgacggc ggcgggccga cgcaccggcg acagaatcga tccgttggca
     2641 cgcgccggcg aagtgctcgg cgagctcg
//
LOCUS       DATLEE010000738         5266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3683851, whole genome shotgun sequence.
ACCESSION   DATLEE010000738 DATLEE010000000
VERSION     DATLEE010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3683851"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /gene="nuoG"
                     /locus_tag="VM692_16185"
     CDS             complement(<1..1714)
                     /gene="nuoG"
                     /locus_tag="VM692_16185"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HUQ53769.1"
                     /translation="MSTPAPTTGPPPSAPAPPVDPNVVNVEVNGVPLKARKGQMIIEV
                     TDQADIYVPRFCYHPKLSIAANCRMCLVEVEKAPKPLPACATPVAEGMKIFTRSKRAI
                     SAQKATMEFLLINHPLDCPICDQGGECELQDLAMGFGRGVSRYTERKRIVKDKNLGPL
                     VSTDMTRCIHCTRCVRFGAEVAGIQELGANFRSDRSEIGTYIEKSVDHELSGNIIDLC
                     PVGALNNKPYRYSARAWEMVAKPLVSPHDCAGSNLYAHVLRGRVRRVVPRDNEAINEA
                     WLADRDRFSCHGVYSADRLLKPRLKHGNDWVEVTWQDALGAAVGALKKATAGGGASGL
                     GTLVSPSATLEEAYLLQRITRHLGSDNIDYRLRQRDFRDQGNDPKVPLLGCSIAELET
                     RQGIFVVGSNLRMEVPIVAHRVRKAARNGASVAFLNTASYEYYFKLAAYVTAPADSFV
                     ANLAAVLAAAATAAGTSVPAQLAGAVADVTVSDAHRAAAQALTRKPGLVLLGHIAQRH
                     PQYADIRALAAGLASITGASLGYLSEGANAAGAALAGATPHRGPGGRGVVRGGFDARA
                     MLETP"
     gene            complement(1711..3000)
                     /gene="nuoF"
                     /locus_tag="VM692_16190"
     CDS             complement(1711..3000)
                     /gene="nuoF"
                     /locus_tag="VM692_16190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HUQ53770.1"
                     /translation="MVCFETLQFGAQSHTYESYRKIGGYDVWEKVLRGELTRETIIDE
                     LKKSGLRGRGGAGFPTGVKMSFMPRTAPMQKYLVCNSDESEPGTCHDRDILRYNPHAL
                     VEGLALESYAIGATVAYNYIRGEFMAEPIPRFEAALKEAYAAGLLGKNIRGSGIDQDI
                     YTFIGAGAYICGEETALLESLEGKQGKPRFKPPFPANFGLYGAPTTINNTQSVASLPT
                     ILRKGAQWFADLGVKGSGGTAIFSMSGHVEKPGNYELPLGIPFPELLAIAGGMRGGRK
                     LKAVIPGGSSCPVVPAEKMMTLTMDFDTVKAAGSGLGTCATIVMDETTCMVKTLRRLA
                     RFYHAESCGQCTPCREGTGWLHRVLTRIIDGKGTKVDIDMLVDVANKIEGHTICALGD
                     AAAWPVQSFIKHFRHEFEYMVENKGRSIVDDAPSVAA"
     gene            complement(3026..3532)
                     /locus_tag="VM692_16195"
     CDS             complement(3026..3532)
                     /locus_tag="VM692_16195"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HUQ53771.1"
                     /translation="MSEHTITPGTLSAHVREEIDHWVAKFPEGWQRSAVIGALHAAQH
                     ENGGYLTPELMIAVAEYLQLPPIQVFEVASFYSMFETRPVGRHSISVCTNISCMLRGG
                     YDILAHVEKRLGIKSGQSTPDGKFYLKIEEECLAACCGAPMMMVDHVYHENLTPKSVD
                     EVLAAVKD"
     gene            complement(3529..4782)
                     /locus_tag="VM692_16200"
     CDS             complement(3529..4782)
                     /locus_tag="VM692_16200"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HUQ53772.1"
                     /translation="MPEIHNFTLNFGPQHPAAHGVLRLVLELDGEVIQKADPHVGLLH
                     RATEKLAETKPFNQSIGYMDRLDYVSMMCNEHAYVLAIERLLGMTPPIRAQYIRVMFD
                     EITRLLNHLMWLGAHGLDIGAMAMFLYCFREREDLMDCYEAVSGTRMHATYYRPGGVR
                     LELPDSMPKYQPAPHRDAKELERINAARTGSLLDFIEDFTQRFPAHVDEYETLLTDNR
                     IWKQRTVNIGVVSPERAMQLGFSGPMLRGSGIAWDLRKQQPYEVYDKVDFDIPVGVNG
                     DCYDRYLVRVEEMRQSNRIIKQCIDWLRVNPGPVAVEDRKVVPPRRIEMKDDMESLIH
                     HFKLFTEGYCVPEGEAYAAIEGPKGELGVYLISDGANKPYRLKVRAPGFPHLAALNEM
                     VVGHMLADVVAVIGTQDIVFGEIDR"
     gene            complement(4775..>5266)
                     /locus_tag="VM692_16205"
     CDS             complement(4775..>5266)
                     /locus_tag="VM692_16205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HUQ53773.1"
                     /translation="KATGTGFSRGTHPGSPPAVEDVGQPTYRPPSRFAVVYHLLSVTH
                     NHRLRLKTFAANDERPIVDSVYDVWNAADWFEREAFDLYGILFRGHPDLRRLLTDYGF
                     IGHPFRKDFPMIGNVEMRYDPVKGRVVYEPVSIEPRTLVPKVIRDDARYEPDLKSRDP
                     GSA"
BASE COUNT          863 a         1713 c         1720 g          970 t
ORIGIN      
        1 gcggcgtctc gagcatcgcg cgcgcgtcga acccgccgcg cacgacgcct ctgccgccgg
       61 ggccgcggtg aggtgtcgca ccggccagtg ccgcgccggc ggcgttggcg ccttcgctca
      121 agtaaccgag cgacgcaccc gtgatgcttg ccaacccggc ggccagcgcg cggatgtcgg
      181 cgtactgcgg atggcgctgt gcgatatggc cgagcaacac gaggccgggc ttacgtgtga
      241 gggcttgtgc ggcggcgcga tgcgcgtccg agaccgtgac gtctgcgacg gcgcccgcaa
      301 gctgcgccgg cacactcgtg ccggccgctg tcgccgccgc ggcgagcacg gccgccaagt
      361 tggcgacgaa gctatcggcg ggcgcggtca cgtaggctgc gagcttgaag taatactcgt
      421 agctcgcggt gttcaggaac gcaacgctcg caccgttgcg cgcggccttg cgcacgcgat
      481 gcgcgacgat cggcacttcc atgcgcaggt tcgagccgac gacgaaaatt ccctggcgcg
      541 tctcgagctc ggcgatgctg caaccgagca gcggcacctt cggatcgtta ccttggtcgc
      601 ggaagtcgcg ttgccgcagc cggtagtcga tgttgtcgct gccgagatgg cgcgtgatgc
      661 gctgcagcag atacgcttct tcgagtgtcg cgctcgggct cacgagcgtg ccgagcccgc
      721 ttgcaccgcc gcctgcagtg gcctttttca gcgccccgac ggccgcgccc agtgcgtctt
      781 gccacgtgac ttcgacccag tcgttgccgt gcttgaggcg cggcttcagc aaacggtcgg
      841 cgctgtagac gccgtggcag ctgaagcgat cgcggtcggc aagccaggcc tcgttgatcg
      901 cctcgttgtc gcgcggcacg acgcggcgca cccggccgcg tagcacgtgc gcatagaggt
      961 tgctgccggc gcagtcgtgc gggctcacga gcggcttcgc gaccatttcc caagcgcgcg
     1021 cgctgtagcg gtacggcttg ttgttcaacg cgccgacggg gcacagatcg atgatgttgc
     1081 ccgaaagctc gtggtcgacg ctcttctcga tgtacgtgcc gatctcggag cgatcgctgc
     1141 ggaagttggc gccaagctct tgaatgccgg cgacttccgc gccgaaccgc acgcaacgcg
     1201 tgcagtggat gcagcgcgtc atgtccgtcg acacgagcgg ccccaagttc ttgtctttga
     1261 cgatgcgctt cctctcggtg tagcgcgaga cgccgcggcc gaagcccatc gcgagatcct
     1321 gcagctcgca ctcgccgccc tgatcgcaga tcgggcagtc gagcggatga ttgataagca
     1381 ggaactccat cgtcgctttc tgcgccgaga tcgcgcgctt cgagcgcgtg aagattttca
     1441 ttccctcggc gaccggcgtc gcacaggccg gcaaaggctt cggcgccttc tcgacctcga
     1501 ccaagcacat tcggcaattg gccgcgatcg acagcttcgg gtggtagcag aacctcggca
     1561 cgtagatgtc ggcctggtcc gtcacctcga tgatcatctg gcccttgcgc gccttcaggg
     1621 gcacgccgtt gacctcgacg ttgacgacgt tgggatcgac aggcggcgcc ggcgccgagg
     1681 gcggcggtcc cgtcgtgggg gccggcgtgc tcatgcggcc accgacggcg cgtcgtcgac
     1741 gatgcttctg cccttgttct cgaccatgta ctcgaactcg tggcggaagt gcttgatgaa
     1801 gctctgcacc ggccatgcgg ccgcgtcgcc gagcgcgcag atcgtgtggc cctcgatctt
     1861 gttggcgacg tcgacgagca tgtcgatgtc gaccttcgtg cctttgccgt cgatgattcg
     1921 cgtcaacacg cggtgcagcc agcccgtgcc ttcgcggcac ggcgtgcact gaccgcacga
     1981 ctcggcgtga taaaagcgcg cgagcctgcg cagcgtcttg accatgcacg tcgtctcgtc
     2041 catgacgatc gtcgcgcagg tgccgaggcc cgagcccgcc gccttgacgg tgtcgaagtc
     2101 catcgtcagc gtcatcatct tctcggcggg gaccacgggg cacgacgagc cgccggggat
     2161 cacggccttc agcttgcggc caccgcgcat gccgccggcg atcgcgagca actcgggaaa
     2221 cgggatgccg agcggcagct cgtagttacc gggcttctcg acgtggcccg acatcgagaa
     2281 gatggccgtg ccgcccgagc ctttgacgcc gaggtcggcg aaccattgcg cgcctttgcg
     2341 caagatcgtc ggcagcgacg caacgctctg cgtgttattg atcgtcgtcg gcgcgccgta
     2401 gagaccgaag ttcgcaggga acggcggctt gaagcgcggc ttgccttgct tgccctcgag
     2461 cgactcgagc agcgccgtct cctcgccgca aatgtaggcg ccggcgccga tgaacgtgta
     2521 aatgtcctga tcgatgccgc taccgcggat gttcttgccg agcagtcccg ccgcatacgc
     2581 ttctttgagc gccgcctcga accgcgggat cggttcggcc atgaactcgc cgcggatgta
     2641 gttgtaggca accgtcgcgc cgatcgcgta gctctcgagc gcgaggccct cgacgagcgc
     2701 gtgcgggttg tagcgcagga tgtcgcggtc gtggcacgtg ccgggctcgc tctcgtccga
     2761 gttgcagacg aggtatttct gcatcggcgc cgtgcgcggc atgaagctca tcttcacgcc
     2821 ggtcgggaat cccgcaccgc cgcggccgcg caagcccgac ttcttgagct cgtcgatgat
     2881 cgtctcgcgc gtcagctcgc cgcgcagcac tttctcccag acgtcgtagc cgccgatctt
     2941 gcggtagctc tcgtaggtgt gcgactgagc gccgaactgc agcgtctcga agcaaaccaa
     3001 gttgaactgt cgttccgcca cgccttcaat cctttacggc tgcgaggacc tcgtcgaccg
     3061 acttcggcgt gaggttctcg tgatacacgt gatcgaccat catcatcggc gcgccgcagc
     3121 aggccgcgag gcactcttcc tcgatcttca aatagaactt gccgtcgggc gtgctctgcc
     3181 ccgatttgat gccgagccgc ttctcgacgt gggcgaggat gtcgtagccg ccgcgcagca
     3241 tgcaggagat gttcgtgcag accgagatac tgtgccggcc gacgggcctc gtctcgaaca
     3301 tcgagtagaa ggacgccacc tcgaacacct ggatcggcgg caactgcagg tactcggcga
     3361 ccgcgatcat gagctccggc gtcagatacc cgccgttctc gtgctgagcg gcgtgcaacg
     3421 cgccgatgac ggccgagcgc tgccacccct cgggaaattt cgcgacccag tggtcgattt
     3481 cctcccggac gtgcgcagac aacgtgcccg gtgtgatcgt gtgctcgctc atcgatcgat
     3541 ctcgccgaag acgatgtctt gagtgccgat gacggccacg acgtcggcga gcatgtgacc
     3601 gacaaccatc tcgttgagcg ccgcgaggtg cggaaacccg ggcgccctca ccttcagtcg
     3661 gtacggctta ttggcgccgt ccgagattag atatacgccg agctcgcctt tcggcccttc
     3721 gatcgcggcg taggcctcgc cttcgggcac gcagtagcct tccgtgaaca atttgaagtg
     3781 atggatcaag gactccatgt cgtccttcat ctcgatgcgg cgcggcggca cgaccttgcg
     3841 atcctcgacg gcgaccggac cggggttcac gcgcagccaa tcgatgcact gcttgatgat
     3901 gcggttcgac tggcgcattt cctcgacacg gacgagatag cggtcgtagc aatcgccgtt
     3961 gacgccgacg gggatgtcga agtcgacctt gtcgtagacc tcgtagggct gctgcttccg
     4021 taggtcccat gcaatgcccg agccgcgcag catagggccc gagaagccga gctgcatcgc
     4081 acgctccggc gacacgacac cgatgttgac cgtgcgctgc ttccagatgc gattgtcggt
     4141 cagcagcgtt tcatactcgt cgacgtgcgc cgggaaacgt tgcgtgaagt cctcgatgaa
     4201 atcgagcagc gagcccgtgc gggccgcgtt gatgcgctcg agctccttgg cgtcccggtg
     4261 cggcgccggc tggtacttcg gcatgctgtc tgggagctcg aggcgcacgc cgccgggccg
     4321 gtagtacgtc gcgtgcatgc gcgtgccgga gacggcctca tagcaatcca tgaggtcctc
     4381 gcgctcgcgg aagcagtaca agaacatcgc catcgcgccg atgtcgagcc cgtgcgcgcc
     4441 gagccacatc aagtgattca gtaggcgcgt aatctcgtcg aacatcaccc ggatgtactg
     4501 cgcgcgaatc ggcggcgtca taccgagcag tcgctcgatg gcgagcacat acgcgtgctc
     4561 gttgcacatc atcgacacgt agtccagccg gtccatgtag ccgatcgact gattgaacgg
     4621 cttcgtctcg gccagcttct cggtcgcgcg gtgcagcaag ccaacgtgcg gatcggcctt
     4681 ttgaatgacc tcgccgtcga gttcgagcac gagcctcagc acgccatgcg ctgccgggtg
     4741 ctgaggaccg aagttcagcg tgaaattatg gatctcaggc actgccgggg tctctcgact
     4801 tcaaatccgg ctcgtagcgc gcgtcgtcgc ggatgacctt cggcacgagc gtgcgcggct
     4861 cgatgctgac gggctcgtag acgacgcggc ccttgacggg gtcgtaacgc atctcgacgt
     4921 tgccgatcat cgggaagtcc ttgcggaacg gatgaccgat gaagccgtag tccgtcagca
     4981 gccggcgcag atccgggtga ccgcggaaca agatcccgta cagatcgaac gcctcgcgct
     5041 cgaaccagtc ggccgcattc cagacgtcgt aaacggagtc gacgatcggc cgctcgtcat
     5101 tcgcggcaaa tgttttcagg cgcagacgat gattgtgcgt gaccgagagc aggtggtaga
     5161 cgacagcgaa ccggctcgga ggccggtacg tgggctgccc cacgtcctcg acagccggcg
     5221 gagaacccgg atgcgtgccg cggctaaagc ccgtacccgt ggcctt
//
LOCUS       DATLEE010000739         2718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6483999, whole genome shotgun sequence.
ACCESSION   DATLEE010000739 DATLEE010000000
VERSION     DATLEE010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6483999"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1026
                     /locus_tag="VM692_16210"
     CDS             133..1026
                     /locus_tag="VM692_16210"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014685701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HUQ53774.1"
                     /translation="MSERNRGFIYRATAVTADAGQSVLAFHVARFRHSGEAEWRAAID
                     MGRVLVNGRRAAPEQRLAAGDELEFHRPPWDEPDAPESFDVAFEDEHVLVVVKPAGLQ
                     VLPAGPFSERTLLTLVRESDASRAAAAPAHRLGRGTSGLIAFGKSAAGRSALTRQLRE
                     LTLGKTYLAWVAGARLPDSFAARQPIARVPHGPLTIHAAATHGRPSLTRVRVLRRAGE
                     RTLVAAQPITGRPDQIRIHLAAAGAPIVGDPLFGPGGVPISDAPPGRGGYLLHAAALS
                     FDHPTSGARVRLRSRPDWLAS"
     gene            1046..2287
                     /locus_tag="VM692_16215"
     CDS             1046..2287
                     /locus_tag="VM692_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53775.1"
                     /translation="MRASTVGRVAVIVAAGGVLAACDGDRAQSAAALRTQDVAFPGGA
                     GSAEPRLTSGPDGTPVLSWLEPAGDAAVLRFSTFASGAFGTPHEVTRGDDLMVNWADL
                     PSVQPIARDVWAAHWLKNTPDPSGAYNIVTAVSADGGRTWTSPAQLNDDDTMAEHGFV
                     ELFAWNDSIAAFWLDGRQLAEFSFDEPDKLLGTSLRIARLDESGAAVSREIVDELVCD
                     CCAPDHAMASAGPVVVYRDRTPEEIRDIAVRRFADGRWHDAVAAGTDNWHIEGCPVNG
                     PKVAAAGARVGVAWFAAPEGAARVRFAWSTDGAASFAPALALDGAGSFGQVGLLLAAD
                     GTAIVSWWRSAQGGGTDLVLRTVGADSALGKPQVVAHSAAVQPVDVPQLVRAGDDVLV
                     AWTNLDDETVHTLLVQSAPRG"
     gene            2385..>2718
                     /locus_tag="VM692_16220"
     CDS             2385..>2718
                     /locus_tag="VM692_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53776.1"
                     /translation="MRISTTSAATWVGVAALCVACTAAAQHEHQPLPPAATELHPGLG
                     DYHFPITTANADAQLYFDQGIRLLYGFNHDEAARYFRRAAELDAQAPMPYWGVALSIG
                     PNYNDTAVD"
BASE COUNT          353 a          972 c          987 g          406 t
ORIGIN      
        1 tagcctcgag ccccgttggg ccgcgctcgc gacgcacgtg ttaccgtcga cgagccccgc
       61 gtacgcgagc ctgcgcttcg agcagaagct cgagcggtgg tcggcggttc tgcgcccgtt
      121 gttggtcgag tcatgagcga aaggaacaga ggtttcatct accgcgcgac ggccgtaacg
      181 gccgacgcgg ggcagagcgt gctcgcgttt cacgtcgcgc ggttccgcca ctccggcgag
      241 gccgagtggc gcgccgcgat cgacatgggc cgcgtgctcg tcaacggccg ccgcgccgcg
      301 cccgagcagc ggctggccgc cggcgacgag ctcgagttcc accggccgcc gtgggacgag
      361 ccggatgcgc cggagtcgtt cgacgtcgcg ttcgaggacg agcacgtgct cgtcgtcgtg
      421 aagccggcgg gcctgcaggt gctgcccgcg gggccgttct cggagcgcac gctgctgacg
      481 ctcgtgcgcg agagcgacgc gtcgcgcgcc gcggccgcgc ccgcgcatcg ccttggccgc
      541 ggcacgtcgg ggctcatcgc gttcggcaag agcgccgccg ggcgctcggc gctgacgcgg
      601 cagctgcgcg agctgacgct cggcaagacg tatctcgcgt gggttgccgg cgcgcgcttg
      661 cccgattcgt tcgccgcgcg gcagccgatc gcgcgcgtgc cgcacggccc gttgactatt
      721 catgccgcgg cgacgcacgg ccggccgtcg ttgacgcgcg tccgcgtgct gcgccgcgcc
      781 ggtgagcgca cgctcgtcgc ggcgcagccg atcacgggcc ggcccgatca gatccgcatc
      841 catctcgccg cggccggcgc gccgatcgtc ggcgatccgt tgttcggccc gggcggcgtg
      901 ccgatcagcg acgcgccgcc gggccgcggc ggctatctgt tgcacgcggc tgcgttgtcg
      961 ttcgaccatc cgacgagcgg cgcgcgcgtc agactgcgtt cgcggccgga ttggttggcg
     1021 agctgacgcg gagtagattg gctctatgag agcatcgaca gtgggccggg ttgccgtcat
     1081 cgttgcggcc ggcggcgttc tcgccgcgtg cgacggcgac cgcgcgcaga gcgccgcggc
     1141 gttgcggacg caggacgtgg cgttccccgg cggcgccggc agcgccgagc cgcggcttac
     1201 gtcgggcccg gacggcacgc ccgtcttgag ttggctcgag cccgccggcg acgcggcggt
     1261 gttgcgtttt tcgacgttcg caagcggcgc gttcggcacg ccacacgaag tcacgcgcgg
     1321 cgacgacctc atggtcaact gggccgactt gccgtcggtg cagccgatcg cgcgcgacgt
     1381 ttgggccgcg cactggctca agaacacacc cgacccgagc ggcgcctaca acatcgtcac
     1441 ggccgtctcg gccgacggcg gccgcacgtg gacctcgccg gcacagctca acgacgacga
     1501 cacgatggcc gagcacggct tcgtcgagct gttcgcctgg aacgattcga ttgcggcgtt
     1561 ttggctcgac ggccgccagc tcgccgagtt ctcgttcgac gagcccgaca agctgctggg
     1621 tacgagcttg cgcatcgcgc ggctcgacga gtccggcgcg gccgtctcgc gcgagatcgt
     1681 cgacgagctc gtctgcgact gctgcgcgcc cgatcacgcg atggcgagcg cgggccccgt
     1741 cgtcgtttat cgcgatcgca cacccgaaga gattcgggac atcgccgtcc gccgcttcgc
     1801 ggacggccgc tggcacgacg ccgtcgctgc cggaacggac aactggcaca tcgaaggctg
     1861 ccccgtgaac gggccgaagg tcgcggcggc cggagcgcgc gtcggcgtcg catggttcgc
     1921 cgcgcccgag ggcgccgcgc gcgtgcgctt cgcatggtcg acggacggcg ccgcgagctt
     1981 cgcgccggcg ctcgcgctcg acggcgccgg ctcgttcggc caagtcgggc tgctgctcgc
     2041 tgccgacggc acggcgatcg tgagttggtg gcggtcggcg caaggcggcg gcacggatct
     2101 cgtgttgcgc accgtcggcg cggacagcgc gctcggcaag ccgcaggtcg tcgcgcacag
     2161 cgcggccgtg caacccgtcg acgtgccgca gctcgtgcgc gccggcgacg acgtgctcgt
     2221 tgcgtggacg aacctcgacg acgagacggt tcacacgctg ctcgtgcagt ccgcgccgcg
     2281 cggctagcga cgcggcgaaa cccgcttcgc ccggacgagc gcatcaatgc tccgcgctcg
     2341 acgggcgctg acagccgtgc cgtttaggcg caagatctcg gcccatgcgt atctcgacga
     2401 ccagtgcggc cacgtgggtg ggcgtcgccg cgctctgcgt tgcttgcacc gccgcggccc
     2461 aacacgagca tcaaccgttg ccgccggcgg cgacggagct gcatcccggg ctcggggatt
     2521 atcacttccc gatcacgacc gcgaacgcgg acgcgcagct ttatttcgac caaggcatcc
     2581 gcctcttgta cggcttcaac cacgacgaag ccgcgcgcta tttccgccgc gccgcggagc
     2641 tcgacgcgca agcgccgatg ccgtattggg gcgtcgcgtt gtcgatcggg ccgaactaca
     2701 acgacacggc cgtcgacg
//
LOCUS       DATLEE010000740         2593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6484495, whole genome shotgun sequence.
ACCESSION   DATLEE010000740 DATLEE010000000
VERSION     DATLEE010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6484495"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /gene="tmk"
                     /locus_tag="VM692_16225"
     CDS             complement(<1..355)
                     /gene="tmk"
                     /locus_tag="VM692_16225"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HUQ53777.1"
                     /translation="MRGAFISLEGIEGVGKSTNVAFTAEAVRKAGYEVITTREPGGTR
                     LGERVREWILNGDHGRLSAEIEALLMFAARARHLDEVIRPALDAGRWIVCDRFTDATF
                     AYQGGGRGASRTLLEM"
     gene            complement(359..1381)
                     /gene="mltG"
                     /locus_tag="VM692_16230"
     CDS             complement(359..1381)
                     /gene="mltG"
                     /locus_tag="VM692_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HUQ53778.1"
                     /translation="MRAKRIWAALAVLLVLGAAALASAAFAWRDLNTPLRLASDDERL
                     RIASGTPFHRVAAELAERGLFDKPWLLRTYARATGEATRVRAGEYELTAGTTPRTLLD
                     KLVAGNVYLHEITIVEGSRFAEVLAALRSHPAITATALDGAAIMTALGAPGVHPEGQF
                     FPDTYRFPLGTEDVEVLRIAHDALTVRLQAAWRGRSPDLLLKSDYEALILASIIEKET
                     ALPAERRLIAGVFHERLRRNMRLQTDPTVIYGMGEAFDGNLRRQDLERDTPYNSYTRG
                     GLPPTPIALPGAGSLEAAVTPEVSGAIYFVATGRPDGSHYFSKTLEEHDRAVRDYLQE
                     LRSREP"
     gene            complement(1371..2174)
                     /gene="pabC"
                     /locus_tag="VM692_16235"
     CDS             complement(1371..2174)
                     /gene="pabC"
                     /locus_tag="VM692_16235"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:NF004761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HUQ53779.1"
                     /translation="MNGHSTGVDPADRGLAYGDGLFETMAANDGVIRWFDLHLDRLEE
                     GCRRLEIPAPSRSLLAHEIAAHCPREGRAVVKLIVTRGPGARGYLPPEPATPTRVLTI
                     SPWPDYPDSHYRSGISARVCQLRLGENPALAGIKHLCRLEQVLAQLELRGYAVQQGLL
                     LDTSGHVVGGTGSNLFAVHGGELATPLLDRCGIKGVMRRAVLETARELGIRAAERDLA
                     LADVLAADELFVTNALFGIWPVTDIDGRPFAIGSTTRQLMARLEYSDAR"
     gene            complement(2269..>2593)
                     /locus_tag="VM692_16240"
     CDS             complement(2269..>2593)
                     /locus_tag="VM692_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902514.1"
                     /note="FabF; beta-ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HUQ53780.1"
                     /translation="STPLGDLAETIAIKRAFGDAAKKVAVSSTKSTTGHMVGAAGGVE
                     AIFTILALHEQVIPPTINLENPDAACDLDYTPNVARQAKLEAALSNSFGFGGTNGSVA
                     FKRLR"
BASE COUNT          428 a          874 c          879 g          412 t
ORIGIN      
        1 gcatctcgag cagcgtgcgg ctcgcgcctc tgccgccgcc ctggtaagca aacgtcgcgt
       61 cggtgaagcg gtcgcagacg atccagcggc cggcgtcgag cgcggggcga atcacctcgt
      121 cgagatgtcg cgcgcgcgcg gcgaacatca atagcgcttc gatctcggct gacaaccggc
      181 cgtggtcgcc gttcaagatc cactcgcgca cacgctcgcc gaggcgcgtg ccgccaggct
      241 cgcgcgtcgt gatgacttcg tagcctgcct tgcgcaccgc ctcggccgtg aacgcgacgt
      301 tcgtcgactt accgacgcct tcgatgcctt cgagggaaat gaatgcgccg cgcatcggct
      361 acggctcccg cgaacgcagc tcttgcagat agtcgcgcac cgcgcgatcg tgctcttcga
      421 gagtcttcga gaaatagtga ctgccgtcgg ggcggcccgt cgcgacgaaa tagatcgcgc
      481 cgctgacctc gggcgttact gccgcctcga gcgagccggc gcccggcaag gcgatcggtg
      541 tcggcggcaa gccgccgcgc gtgtacgagt tgtaaggcgt gtcgcgttcg agatcttgcc
      601 ggcgcagatt gccgtcgaac gcttcgccca ttccatagat gaccgtggga tcggtctgca
      661 agcgcatgtt tcgccgcaac cgctcatgga aaacgcccgc gatcaaccgc cgctccgcgg
      721 gcagcgccgt ctccttctcg atgatcgacg ccaggatcaa cgcctcgtaa tcgctcttca
      781 gcagcaggtc ggggctgcgg ccgcgccacg ccgcttgcag ccgcaccgtc agcgcgtcgt
      841 gcgcgatgcg tagcacctcg acgtcctcgg tgccgagcgg aaaacggtag gtgtccggga
      901 agaactgtcc ttcgggatgc acgccgggcg cgccgagcgc ggtcatgatt gccgcgccgt
      961 cgagcgccgt ggcggtgatg gcgggatgcg agcgcagagc cgcgagcacc tcggcgaagc
     1021 gcgagccttc gacgatcgtg atctcgtgca gatacacgtt gccggcgacg agcttgtcga
     1081 gcagcgttcg cggtgtcgtg ccggccgtca gctcgtactc gccggcgcga acgcgcgtcg
     1141 cctcgccggt agctcgcgcg taggtccgca gcaaccacgg cttgtcgaac aagccgcgct
     1201 cggcgagctc ggccgcgacg cggtggaacg gcgtgccgct cgcgattcgc agccgctcgt
     1261 cgtcggaagc caagcgcaac ggcgtgttga ggtcgcgcca agcgaatgcc gcagaagcaa
     1321 gcgccgctgc gccgagcacc agcaacacgg cgagcgccgc ccaaattcgc ttagcgcgca
     1381 tcggaatact cgagccgcgc catgagctgc cgggtcgtag aaccgattgc gaagggccgg
     1441 ccgtcgatgt cggtgacggg ccaaatgccg aacagcgcgt tggtgacgaa cagctcgtcc
     1501 gctgccaaca cgtcggcgag cgcaagatcg cgctccgcgg cgcggatacc gagctcgcgt
     1561 gcagtctcga gcacggctct gcgcatgacg cccttgatgc cgcagcgatc gagcagcggc
     1621 gtcgcgagct cgccgccgtg cacggcgaac aaattgctgc cggtgccgcc gacgacatgg
     1681 ccgctcgtgt cgagcaacag cccttgctgc acggcgtagc cgcgcagctc gagctgcgcc
     1741 agcacttgct cgaggcggca gagatgcttg atgcctgcga gggccgggtt ctcgccgagc
     1801 cgcaactggc acacgcgcgc actgatgccc gaacgatagt gcgagtcggg atagtcgggc
     1861 cacggcgaga tcgtcagcac gcgcgtcggt gtcgcgggct caggcggcaa gtagccgcgc
     1921 gcgccggggc cgcgcgtgac gatgagcttc acgactgcgc ggccctctct cgggcaatgc
     1981 gcggcgatct cgtgcgccag caggctgcgg gacggagcgg gtatttccaa gcgccgacaa
     2041 ccttcctcga gtcggtcgag atgcagatcg aaccaacgaa tgacgccgtc gttggcagcc
     2101 atcgtctcga ataggccgtc accgtacgcg agcccgcggt ccgcgggatc gaccccggtc
     2161 gagtgtccgt tcacgagcca gagcgtgttc acggtgcaac gcccgcgact aatcagtccg
     2221 tcttgtaaca gatggaggcg cacccgtcat gtcgacagcg cgcaaagctc agcgcaagcg
     2281 cttgaaggcc accgagccgt tcgtgccgcc gaagccgaac gagttcgaca acgcggcttc
     2341 gagcttggct tgccgcgcga cgttcggcgt gtagtcgagg tcgcacgcgg cgtccggatt
     2401 ctcgaggttg atcgtcggcg gaatgacctg ctcgtgcagc gccaggatcg tgaagatggc
     2461 ctcgacgccg ccggcggccc cgaccatgtg gcccgtcgtc gacttcgtcg agctgacggc
     2521 taccttcttg gccgcatccc cgaacgcacg cttgatcgct atcgtctccg cgaggtcgcc
     2581 gagcggcgtg gag
//
LOCUS       DATLEE010000741         3579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3695087, whole genome shotgun sequence.
ACCESSION   DATLEE010000741 DATLEE010000000
VERSION     DATLEE010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3695087"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VM692_16245"
     CDS             complement(<1..646)
                     /locus_tag="VM692_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUQ53781.1"
                     /translation="MAVGLSIVLSGCTTLDAYTREERPARAQRQAVIGAAAGAVAGLI
                     TGDSSMERKKRALVGAGLGALAGAGVGAYQDRQEARLRAELERTGVSVTRIGDNITLN
                     MPGNVTFATGSSDLNASFFEVLNSVATIVNEYNQTVIEVAGHTDSVDTEAYNQALSER
                     RANAVAAYLRTRSVREDRIITVGEGEKRPIATNDTDAGRQQNRRVELTLVPLTLE"
     gene            810..1508
                     /gene="pdsR"
                     /locus_tag="VM692_16250"
     CDS             810..1508
                     /gene="pdsR"
                     /locus_tag="VM692_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HUQ53782.1"
                     /translation="MSTAKLVAVVEDEVAIRDNYLAGLRRHGYRVVGYGSRKEALAGF
                     ATRFPDLVIIDVNLGNEVEGGFELCRELRARSAQLPIVFLTARESELDAVSGLRLGAD
                     DYLTKRTSIEHILARVAALFRRVEALRSGDAPSERLEQGSLALDRDRMTAQWKSTSVP
                     LTVTEFWVVHALALHPGHVRSREQLMAAANVVLDDGTVTSQIKRIRAKFVGVDPKFDA
                     IQTVYGMGYRWQSD"
     gene            1825..>3579
                     /locus_tag="VM692_16255"
     CDS             1825..>3579
                     /locus_tag="VM692_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HUQ53783.1"
                     /translation="MRKANTTGLPPAQGLYRPEFEHDSCGVGFVAHIKGKRSHKIIED
                     ADHLLCRMDHRGARGAEPNTGDGAGMVTALPHEFLTRVARECFSVELPAPGKVAAGVV
                     FLPPDAGERERCKSMVAKLCAEEGQVLVGWRVVPTDADAADLGHGARAAAPHIEQLFV
                     AAGEGFDGEAFERKLYLLRKRASHLLRGDKTLAQAKHFYVCSLSTKVLIYKGMLTPGQ
                     LLNFYPDLRAPDYTTHLAMVHSRFSTNTFPSWDRAQPNRFMSHNGEINTLRGNENWMR
                     ARQGVVATPLFGDDLKKLFPIAEPDCSDSGVFDNVLEFLLMTGRSLQESVMMMIPEAW
                     QKNELMPENKRAFYEYHSCLMEPWDGPASIAFTDGKCIGAVLDRNGLRPSRYYLTHDD
                     RVIMASEVGVVPIDPRNVKAKGRLEPGRMFLVDFEHGRLVPDDELKNEFANRRPYGEW
                     LEANRLALRPLAEAVPALPEIDRSTLMPRLQAFGYTIETLHFMLQPMVREHRDPVGSM
                     GNDSALAVLSDKPRMLYDYFRQLFAQVTNPPIDSIREEVIMSLECYVGPEGNLLETTP
                     KHAERLRMRQPILSNEELA"
BASE COUNT          619 a         1201 c         1145 g          614 t
ORIGIN      
        1 tttccagcgt cagcggtacg agcgtgagct cgactcgacg gttctgctgc cgcccggcat
       61 ccgtatcgtt ggtcgcgatg gggcgctttt ccccctcgcc gaccgtaatg atgcggtcct
      121 cgcggacgct gcgcgtcctc aagtacgcgg cgaccgcgtt ggctcggcgc tccgaaagcg
      181 cttggttgta ggcttcggta tcgacgctgt ccgtgtggcc tgccacctcg atgaccgtct
      241 ggttgtactc attgacgatc gttgcgacgg aattgagcac ttcgaagaag ctcgcgttca
      301 aatcggagct gcccgtggca aacgtaacgt tgcccggcat gttcagcgta atgttgtcgc
      361 cgatgcgcgt gacgctgacg ccggtacgct cgagctctgc tcgcagccgc gcttcctgac
      421 ggtcctggta cgcgccgacg ccggcgcctg ccagcgcccc gagacccgcg ccgaccagcg
      481 cgcgcttctt gcgctccatc gagctgtcgc ccgtgatgag gccggcgacc gcgcccgcgg
      541 cagcgcctat tacggcttgg cgctgcgctc gagcgggccg ttcctcacgg gtgtaagcgt
      601 cgagcgtcgt gcaaccggat aacacgatgc ttagcccgac cgccatggca cccactctcg
      661 accaattctg aatccccatg tcccgtacct cctcctgtta tgagcagcgg tatcatgcca
      721 agcccaacct gaacgaaacg tgacctggca cccccacact atgccaaagt catgcccgcc
      781 ttagcccggg ccgccctccg atccaacaga tgtcaaccgc caagctcgta gccgtcgtag
      841 aggacgaggt cgcgattcgc gacaactacc tggccgggct gcgccggcac ggctaccggg
      901 tcgtcggtta cgggagccgc aaggaggcgc tggccggctt tgcgacgcgc tttccggatc
      961 tcgtcatcat cgacgtcaac ttgggcaatg aggtcgaagg ggggttcgag ctgtgccgcg
     1021 agctgcgtgc ccgctcggcg cagctgccga tcgtctttct gacggcgcgc gagagcgagc
     1081 tcgacgcagt ctcgggcctg cgcctcggcg ccgatgacta cctcaccaaa cgcaccagca
     1141 tcgagcacat actcgcccgc gtggccgccc tgtttcggcg cgtcgaggcg ctgcgctccg
     1201 gcgatgcgcc gagcgagcgg ctcgagcagg gctcgctcgc cctcgatcgc gatcgcatga
     1261 ccgcgcagtg gaaatcgacg agcgtgccgc tgaccgtcac ggagttctgg gtcgtgcacg
     1321 cgctcgcact gcatccgggc cacgtccgca gccgcgagca actcatggcg gccgcgaacg
     1381 tggtgctcga cgacggcacc gtcacgtcgc aaatcaagcg catccgggcg aaattcgttg
     1441 gcgtcgatcc caagttcgac gcgatccaga cggtctacgg catgggctac cgctggcaat
     1501 cggattgacc cccggcgccc gacgccacga ttgacgcgta gatggcgcct tccctatagt
     1561 ctgacgttgg cacaaggcct gctttcggtc ctttattcag gacctctccg cggcagatcg
     1621 aatggccggc ggcttgacag gccagcatcg cagctgccag ccacctaaaa agaaatgagc
     1681 cgagcggagc cagacggctg gccgctgtat agaaccatga ctgcaagagc gacgaacacg
     1741 aaactgcatc gagccggcac gcccaaacgg gcgggcgccg accgttgggt cgtgctcgct
     1801 cttgccgcat gaggaacggc aagcatgcgt aaagcaaaca ccaccggcct cccgccggcg
     1861 caagggttgt atcggcccga gttcgaacac gacagctgcg gcgtgggctt cgtcgcgcac
     1921 atcaagggca aacgcagcca caagatcatc gaggacgccg atcacctgct gtgccgcatg
     1981 gatcaccgcg gtgcgcgcgg cgccgagccc aacacgggcg acggggccgg catggtcacg
     2041 gcgctgccgc acgagttcct gacgcgagtc gcgcgcgagt gcttctcggt cgagttgccc
     2101 gcgcccggca aggtcgcggc cggcgtcgtc ttcctgccgc ccgacgcggg cgagcgtgag
     2161 cgctgcaagt cgatggtcgc gaagttgtgc gccgaggaag gccaggtgct cgtcggctgg
     2221 cgcgtcgtgc ccacggacgc cgacgctgcc gatcttggcc acggcgcgcg tgcggccgcg
     2281 ccgcacatcg agcagttgtt cgtcgccgcg ggcgagggtt tcgacggcga agccttcgag
     2341 cgcaagctct acctgctgcg caagcgcgcg agccatctgt tgcgcggcga caagacgctg
     2401 gcccaggcca agcatttcta cgtctgcagc ctgtcgacga aggtgctcat ctacaaaggc
     2461 atgctgacgc cgggtcagct gctgaatttc tatcccgacc tccgcgcgcc ggactacacg
     2521 acgcacctcg cgatggtgca ctcgcgtttt tcgacgaaca cgttcccgag ctgggaccgt
     2581 gctcagccga atcgtttcat gtcgcacaac ggcgagatca acacgttgcg cggcaacgag
     2641 aactggatgc gcgcccggca gggcgtcgtc gctacgccac tcttcggcga cgatctcaag
     2701 aagctgtttc cgatcgcgga gccggactgc tcggactcgg gcgtgttcga caacgtgctc
     2761 gagttcttgc tgatgaccgg ccggtcgctg caggaatccg tgatgatgat gattcccgag
     2821 gcatggcaga agaacgagct catgcccgag aacaagcgcg ccttctacga gtatcactcg
     2881 tgcctcatgg agccgtggga cgggccggca tcgatcgcct tcacggacgg caagtgcatc
     2941 ggggctgtgc tcgaccgcaa cgggttgcga ccgagccgct attacctgac gcacgacgat
     3001 cgcgtcatca tggcgagcga ggtcggcgtc gtgccgatcg accccaggaa cgtcaaggcc
     3061 aagggccgct tggagccggg gcgcatgttc ctcgtcgact tcgagcacgg ccggctcgtg
     3121 cccgacgacg agctcaagaa cgagttcgcg aatcggcgcc cgtacggcga atggctcgag
     3181 gcgaaccgcc tggcgctgcg tccgcttgcg gaggccgtgc ccgcgctgcc cgagatcgat
     3241 cgttcgacgt tgatgccgcg cttgcaggcg tttggctaca cgatcgaaac gctgcacttc
     3301 atgctgcagc cgatggtgcg cgagcatcgc gaccccgtcg gctccatggg caacgacagc
     3361 gcgctcgccg tgctcagcga caagccgcgc atgctctacg attacttccg acagctgttc
     3421 gcgcaggtga ccaatccgcc gatcgactcg atccgcgaag aggtcatcat gtcgctcgag
     3481 tgttacgtcg gcccggaggg caacctcctc gagacgacgc cgaagcacgc cgagcggttg
     3541 cgcatgcgcc agccaatcct gtcgaacgag gagctggcg
//
LOCUS       DATLEE010000742         3514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10221573, whole genome shotgun sequence.
ACCESSION   DATLEE010000742 DATLEE010000000
VERSION     DATLEE010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10221573"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VM692_16260"
     CDS             complement(<1..385)
                     /locus_tag="VM692_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011771046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53784.1"
                     /translation="MAKNKGKAAGKPAKQAAKAAPIRKGGKSQLVEPKQILDDDIDPH
                     HQWKRPLQAPGHTQVDFEERVNFRRLHNYRLARVREALAGSGLGALLSFDQHNIRYTT
                     STVIGEWARDKLTRYSLLTGNGDPYI"
     gene            complement(482..1924)
                     /locus_tag="VM692_16265"
     CDS             complement(482..1924)
                     /locus_tag="VM692_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53785.1"
                     /translation="MAAFAIRLARKFGAAILVLGACTATSAGAALDTGAFDAEQRQLL
                     DRIDEIQSRDGAYSPALLDELKRLIVLYREREDHELALVVIERALQVVHANSGLYTLD
                     QVPLMWQRLESEEARGNDEAAWDVEQELLTLARRHPDDLDAVPVLRAVADRQSAVLDR
                     VVASNEIPPQVILGCYYEEWPNQYDRNCKAGSRTTVVQGMLADADRNYADAIAVLLRN
                     EAYSSDELRELEMELVRGAQLIHEEYKDEGNRGVTGTRTRNPVPVPLTPWSTNAKTLE
                     PWRSRLASIEELADWELPYASAGTPEEHFLQELEPRDARFRVPYYRGRQSLRRLYGYS
                     VASATSPLDQASALVQIADWDLLFSSNGLAVEGYGLAREMLETANVDAASIAELFAPE
                     LPVVLPSFQPNPLLRDETLPARGHIDVAFAITKYGRARAIEIRGAANATDDDEAELES
                     LLKNNRFRPRLKDGKLADTTPVVVRYYLHD"
     gene            complement(2039..3385)
                     /locus_tag="VM692_16270"
     CDS             complement(2039..3385)
                     /locus_tag="VM692_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53786.1"
                     /translation="MTLLALVVFLLPGRSTAAGLADELQDQVQEILSRDGPYSQRLLE
                     PLTTLGLHYQESNDLLLALATLERAVQIVRINSGLHTLEQVPLTRQLIRIEEARGNDT
                     GAWQREQKLLTLLRRHLDDLQTVPVLREIADKQMEVLNAVLAGARPVQVLLGCFYQEW
                     PHQDSADCHSGSRKTVIQGMLAEAQRNYADAIGVLLQHGLYASDELRDLELRVLRGVD
                     LLRTRNEDPSFGYPVPMVPPYLNTASIEPWRSRIAPVAALAEWQPPQHRALAEHIAQG
                     VSSRQVEMMSPYQRGRQSLERIYEYGAASSSPSLSQADAIVQLADWDLLHSHNGQALQ
                     SYTAAYAMLEQAGTAPASIAQLFSPEMPVVLPAFQPNPLLSNETRPPTGHIDVAFEIT
                     QYGRGREIEVRDAANATAEETERLVSLIKSNRFRPRLTDGHLAAAAPVVLRYYVYE"
BASE COUNT          571 a         1140 c         1142 g          661 t
ORIGIN      
        1 agatgtaggg atcgccgttg ccggtgagca gcgagtagcg cgtcagcttg tcgcgcgccc
       61 attcgccgat gacggtgctc gtcgtgtaac gaatgttgtg ttggtcgaac gacagcaaag
      121 caccgaggcc gctaccggcc agcgcctcgc gaacgcgcgc gagacggtag ttgtgcagcc
      181 gacggaaatt gacgcgctcc tcgaagtcga cctgcgtgtg gccgggcgct tgcagggggc
      241 gtttccactg gtggtgcggg tcgatgtcgt cgtccaggat ctgtttcggc tcgacgagct
      301 gcgacttgcc gcctttgcga atcggagccg ctttggcggc ttgcttcgcc ggcttgcccg
      361 cggcctttcc tttgttcttc gccatggttg tcctctattg aatgagcgct gccggaacac
      421 gcgcagttcc gagcgggatt gtacgcggca gcccctggat cgggacagcc tggcgaaccg
      481 gtcagtcgtg cagatagtag cgaacgacga cgggtgtcgt gtcggcgagc ttgccgtctt
      541 tgaggcgcgg ccggaaccgg ttgttcttca acaggctctc gagctcggcc tcgtcgtcgt
      601 ccgtggcatt ggccgcgccg cggatctcga tcgcacgcgc tcgaccgtat ttggtgatcg
      661 caaacgccac gtcgatgtgt cctctggccg gcaaggtctc gtcgcgcaac agcgggttcg
      721 gctggaacga cggcagcacg acgggcaact cgggcgcgaa cagctccgcg atcgatgccg
      781 cgtccacatt cgccgtctcg agcatttccc gcgcgagccc atagccctcg accgctagcc
      841 cgttgctcga gaacagcaga tcccaatcgg ctatttggac gagcgcgctc gcctggtcga
      901 gcggcgaagt cgcgctcgca acgctgtatc cgtagagacg gcgcaagctc tggcggccgc
      961 ggtagtacgg cactctgaaa cgcgcatccc gcggctcgag ctcctgcagg aagtgctcct
     1021 cgggcgtacc cgcgctggcg taaggaagct cccagtcggc cagctcctcg atggatgcca
     1081 ggcggctgcg ccacggctcg agagtcttgg cgttcgtaga ccacggggtc agcggaaccg
     1141 ggactggatt tctcgtgcgc gtgcccgtga cgccgcgatt gccttcgtct ttgtattcct
     1201 cgtggatgag ctgcgcaccg cggacgagct ccatctcgag ctcgcgcagc tcgtcgctcg
     1261 aataagcttc gttgcgcaac aggaccgcga tcgcgtcagc gtagttgcga tcggcgtccg
     1321 cgagcatgcc ttgcacgacc gtcgtccgac tgccggcctt gcagttgcgg tcatattggt
     1381 tcggccattc ctcgtagtag caacccaaga tgacctgcgg cgggatctcg ttgcttgcga
     1441 cgacccgatc gagcaccgcg ctttggcggt ccgccaccgc gcgcagcacg ggcaccgcgt
     1501 cgaggtcgtc gggatgtcgt ctcgccaacg tcagcagctc ctgctcgacg tcccacgccg
     1561 cttcgtcgtt gccgcgagct tcctcgctct cgagccgctg ccacatcagc ggcacttgat
     1621 cgagcgtata gagcccgcta ttggcatgca cgacttgcag agcccgctcg atcacgacga
     1681 gtgccagctc gtggtcctcg cgctcgcggt acagcacgat caagcgcttg agctcgtcca
     1741 acaacgccgg cgagtacgcg ccgtcgcgcg attggatctc gtcgatgcgg tcgagaagct
     1801 gccgctgttc ggcgtcgaac gcgcccgtgt cgagcgccgc gccggcactc gtcgccgtgc
     1861 aagcgccaag cacgaggatc gcggcaccga acttgcgagc caggcggatt gcgaacgcgg
     1921 ccatggcacc cccgcacgct ggagcgtacc gggattctac cgtggcgccc tacggctcgt
     1981 ccgacgcggc cgcaaggtcg gacgcaaagc cagcgcggct cctggcgagc tcgcgaactt
     2041 actcgtacac gtagtagcgc agaacgacgg gtgccgccgc tgccaggtgc ccgtccgtca
     2101 atcgcggccg gaagcgattg ctcttgatca aagagacgag ccgttccgtt tcctcggccg
     2161 tggcgttcgc ggcgtcgcgg acctcgatct cgcggcctcg tccgtactgc gtgatctcga
     2221 acgccacgtc gatgtgccct gtgggcggcc gagtctcgtt gctgagcaac gggttcggct
     2281 gaaacgccgg caacacgacg ggcatctcgg gcgagaacag ctgcgctatc gaggccggcg
     2341 cagtgccggc ctgttcgagc atcgcatagg ctgccgtgta gctctggagc gcttggccgt
     2401 tgtgcgaatg cagcaggtcc cagtcggcga gctgcacgat cgcgtccgct tggctcagcg
     2461 acgggctcga gctcgctgcg ccgtattcat agatgcgctc caagctctgc cgcccgcgct
     2521 gatatggcga catcatctcg acctgcctgg agctcacgcc ctgcgcgatg tgttcagcga
     2581 gcgctcggtg ctgtggcggt tgccactccg cgagcgcggc gaccggagcg atgcggctgc
     2641 gccatggctc gatgctggcc gtgttcagat acggcggcac catcggcacg ggataaccga
     2701 agctcgggtc ctcgttacgc gtgcgcagca agtcgacgcc gcgcaacact cgcaactcca
     2761 agtcgcgcag ctcgtcgctg gcatagaggc cgtgctgcaa caacactccg atcgcgtcgg
     2821 cgtaatttcg ttgcgcctcg gcgagcatgc cttggatgac ggtcttgcga ctgccggaat
     2881 ggcagtcggc cgagtcctga tggggccact cctgatagaa gcagccgagt agcacctgca
     2941 cgggccgtgc gccggccagc actgcattca gcacttccat ctgcttgtcg gcgatctcgc
     3001 gcagcaccgg caccgtttgc aggtcgtcga gatgacgccg caacagcgtc aacagctttt
     3061 gctcgcgctg ccacgcaccg gtgtcgttgc ctcgggcctc ctcgatgcga atgagttggc
     3121 gcgtcagcgg aacttgctcg agcgtgtgca agccgctgtt gatgcgcacg atttgcactg
     3181 cccgttccaa cgtcgcgagc gccaacagca gatcgttgct ctcttgataa tgcaggccca
     3241 gcgtcgtcag cggctcgagt aagcgctgcg agtacggccc atcgcgtgaa agaatttctt
     3301 gaacttgatc ttgcagctcg tcggcgagcc ctgctgcggt cgagcgaccg gggagtagga
     3361 agacgacgag tgcgaggagc gtcacggcag cagcgacaga catctgggcg tgacgtgtaa
     3421 ccagcgagac cataggcacc tccgatcacg aaccggaatg ccccccgttt gaacgagcag
     3481 atgcaagttc taggccgcgc gatctcgacg gttg
//
LOCUS       DATLEE010000743         2306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8904532, whole genome shotgun sequence.
ACCESSION   DATLEE010000743 DATLEE010000000
VERSION     DATLEE010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8904532"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VM692_16275"
     CDS             <1..424
                     /locus_tag="VM692_16275"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53787.1"
                     /translation="AERGCIELKTVAEIQEAYVDDRGNAATRLVPAAKVVPGDEIVWT
                     IVASNVCKTAAGNVAITNPVPAHMRYVGSSAFGPGADIEFSLDGSTFGGPAALVVAEA
                     DGSRRPARADEYSHIRWVLPRPMGPSEQLIVRYRATVL"
     gene            486..1628
                     /locus_tag="VM692_16280"
     CDS             486..1628
                     /locus_tag="VM692_16280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53788.1"
                     /translation="MTTLTRIGLRAALVAVVGFSWWTQASAQLTPAGTSIQNRATVNY
                     SVGGQPQTLIESSPTGNTAPGAGASASTSFVVDNRIDLTVAELSGNATIASPGAAAAV
                     LAFTVTNTGNAPQGYRLTVAEEVGTLLFGNADNANVGLANLQVRVDEDPSSGNGTGND
                     AYDGTETATAVDLLNPGASITVFVVVPTVPLTLVNGNFANVNLQAQTAVGGTNGLTLA
                     VQSPGANNPTTVEVVFADDPLSADATENAADQLAIRSAALTVTKTQAVLNDGFGSASP
                     RAIPGASVEYTITVANSSTTTAANGIAISDPIPANTAFQTGLYAGSSDVAITGGAAAT
                     CVAETPADTNADGCFRNGGNLVVGGAALGSIAALGNVTVRFQVRIN"
     gene            1643..>2306
                     /locus_tag="VM692_16285"
     CDS             1643..>2306
                     /locus_tag="VM692_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53789.1"
                     /translation="MGAAAAARPRETRGTAPHRTVIAPPRQGGAAQSCSARAVCSRPP
                     FLATLSALLLAATAAAAPPGTPITNRAEVQYVAGSGPTTIVSNSVDVVVVPRPSRAAL
                     TLLRSESSGAPGVAQPTQCTSSGTALALPPPIGSSGQPLVLGQALPLGIAATVHGGEA
                     VFVDLLDADRNRDAAAVDAVELVVTAAGGDRETILLAETAVDSGRFAGYVQTRAAATV
                     LGD"
BASE COUNT          375 a          835 c          776 g          320 t
ORIGIN      
        1 cgccgagcgc ggctgcatcg agctcaagac cgtcgccgag attcaagaag cgtacgtcga
       61 cgaccgcggc aatgcggcga cacgtctcgt cccggctgcg aaggtcgtgc ccggcgacga
      121 gatcgtttgg acgatcgtcg cgagcaacgt ctgcaagacg gcggccggca acgtcgcgat
      181 tacgaacccc gtgccggcgc acatgcgtta cgtcggcagc tcggccttcg ggcccggcgc
      241 cgacatcgag ttctcgctcg acggcagcac gttcggcggc cctgcggcgc tcgtcgtcgc
      301 cgaagccgac ggctcgcgcc gccccgcacg cgccgacgag tactcgcaca ttcgctgggt
      361 gctgccgcgg ccgatgggcc ccagcgagca gctgatcgtg cgttatcgcg caaccgttct
      421 ctagtaagtg caacggcctc gatggaccga ggcctcaagg tcaatcagta gcaaggagca
      481 gtgaaatgac taccctcact cgaattggct tgcgcgcagc gctcgtcgct gtcgtaggct
      541 tcagctggtg gacgcaagcc agcgcccagt tgacgccggc cggcacgtcg attcaaaacc
      601 gcgcgaccgt caactacagc gtcggcggtc aaccgcagac gctgatcgag agctcgccga
      661 cgggcaacac ggcgcccggc gccggcgcca gcgcgagcac gtcgttcgtc gtcgacaacc
      721 gcatcgacct cacggtcgcc gagctctcgg gcaacgcgac gatcgcgtcg cccggcgcgg
      781 cggcggccgt gctcgcgttc acggtcacga ataccggtaa cgcgccgcaa ggctatcggc
      841 tgacggtggc cgaggaagtc ggcacgctgc tgttcggcaa cgcggacaac gccaacgtcg
      901 ggctcgcgaa cctgcaagtg cgcgtcgacg aggatccgag ctccgggaac ggcacgggca
      961 acgatgccta cgacggcacc gagaccgcga cggccgtcga cctcctgaac cccggcgcct
     1021 cgatcacggt gttcgtcgtc gtgccgaccg tgccgctgac gctcgtcaac ggcaacttcg
     1081 cgaacgtgaa cttgcaagcg caaacggccg tgggcggcac gaacggcctc acgctcgcgg
     1141 tccagtcgcc gggcgccaac aacccgacga ccgtcgaagt cgtgttcgcc gacgacccgc
     1201 tgagcgccga cgccacggaa aatgcggccg accagctggc gattcgctcg gccgctttga
     1261 ctgtgacgaa gacacaggcc gtgctcaacg acggcttcgg cagcgcgagc ccacgtgcga
     1321 ttcccggcgc gtccgtcgag tacacgatca cggtcgcgaa ctcgagcacg acgacggccg
     1381 ccaatggaat agcgatcagc gatccgattc cggccaacac ggcattccag acgggcctgt
     1441 acgcgggctc gagcgacgtc gcgatcacgg gcggcgcggc ggcgacctgc gtcgcggaga
     1501 cgccggccga cacgaatgcc gatggctgtt tccgcaacgg cggcaacctc gtcgtcggcg
     1561 gggccgcgct cggcagtatc gccgcgctcg gcaacgtgac cgtgcgtttc caggttcgga
     1621 tcaactgatc gcgagctgac gtatgggtgc ggccgcagcg gcgcggccac gcgaaactcg
     1681 cggcaccgca ccgcaccgga cagtcatcgc tcctcctcgg cagggcggag cagcacaaag
     1741 ctgctccgcc cgcgccgttt gttcgcgccc gcctttcctc gcgacgctga gcgcgctgct
     1801 gctcgccgcc acggccgccg cggcaccgcc cggcacgccg atcaccaatc gcgccgaggt
     1861 gcagtacgtc gccggctcgg ggccgacgac gatcgtcagc aactccgtcg acgtcgtcgt
     1921 cgtgccgcgg ccgtcgcgcg ccgcattgac gttgctacgc tccgagtcga gcggcgcacc
     1981 gggcgttgcg cagccgacgc agtgcacgag cagcggcacc gcactcgcgc tgccgccgcc
     2041 gatcggctcg agcggccagc cgctcgtgct cggccaagcg ctgccgctcg gcatcgcggc
     2101 caccgtgcac ggcggcgaag ccgtgttcgt cgacttgctc gacgccgacc gcaatcgcga
     2161 cgcggctgcg gtcgacgcgg tcgagctcgt cgtcacggcc gccggcggcg accgcgagac
     2221 gatcctgctt gcggagacgg ccgtcgactc gggccggttc gcgggctacg tgcagacgcg
     2281 cgccgccgcg acggtgctcg gcgatt
//
LOCUS       DATLEE010000744         4978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7413240, whole genome shotgun sequence.
ACCESSION   DATLEE010000744 DATLEE010000000
VERSION     DATLEE010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7413240"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..472)
                     /locus_tag="VM692_16290"
     CDS             complement(14..472)
                     /locus_tag="VM692_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53790.1"
                     /translation="MTGAELEDLVRRFHACEVPRREWTHAAHLSVGLWHVSRYGAADA
                     LARLRDGIRRLNESNGVVNSAASGYHETITWAYVQLLAAFAERHADAPVAERVARLLA
                     SDLADRGALLKFYSRPHLESADARLGLVEPDLAPLELDSVFARQAGKWFE"
     gene            complement(479..826)
                     /locus_tag="VM692_16295"
     CDS             complement(479..826)
                     /locus_tag="VM692_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HUQ53791.1"
                     /translation="MKYQGSCHCGRVAFEVEGTIDSALACNCSICQRKGSLLWFVPRE
                     DLKLLTPEGNANTYTFNKRAIKHRFCPNCGMHPYGEGTDSKGNQKAAINLRCLEGIDL
                     ESIPVNHFDGRAA"
     gene            complement(852..1211)
                     /locus_tag="VM692_16300"
     CDS             complement(852..1211)
                     /locus_tag="VM692_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53792.1"
                     /translation="MRCRYRAVTLALIVVAGCSRPDASRTSPAITDGWLGRWTGPEGT
                     FLLLEGGNGRYDVTIQNLDGPRVFVGSSMGDQIRFERDGVIESIHATDGAATGMKWLA
                     EKSNCLTVRPGEGYCRD"
     gene            complement(1208..1750)
                     /locus_tag="VM692_16305"
     CDS             complement(1208..1750)
                     /locus_tag="VM692_16305"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUQ53793.1"
                     /translation="MVIFINGSFGIGKTTVARGLVARLPGSVLFDPELHGLVLQRLAR
                     PFKRIDDFQDLRAWRAGSVRLIRLARLLRPTVVVPMAFSNEAYLGEVVDGIRSFDVDT
                     LHFCLTAPLAVVQHRLQQREGRRGPTPWQLRRAAECCTEHQRPQYAVHVATQDRSSHD
                     VVEDMLTQLRSRAPVQAASA"
     gene            complement(1764..1991)
                     /locus_tag="VM692_16310"
     CDS             complement(1764..1991)
                     /locus_tag="VM692_16310"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HUQ53794.1"
                     /translation="MSADERAIRALVATWLAATKRGDTDAVLELMTDDALFLVAARPP
                     MNKAAFAAASHPPRGPRPSCCSRDANLLVNV"
     gene            complement(1981..2106)
                     /locus_tag="VM692_16315"
     CDS             complement(1981..2106)
                     /locus_tag="VM692_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53795.1"
                     /translation="MYGLIGKMKGVPGQRDALIAGFGERFETSPVGRHGLRSHER"
     gene            complement(2172..2783)
                     /locus_tag="VM692_16320"
     CDS             complement(2172..2783)
                     /locus_tag="VM692_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HUQ53796.1"
                     /translation="MRKVDLKKELKHLYQPSVKAVVAVDVPAFEFLMIDGEGDPNTST
                     AYAAAVEALFAVSYTAKFMVKKAQAIDFGVMPLEGLWWADDLSVFVAGDKSKWQWTMM
                     IMQPSFVTSAVIEAALAEVRKKKGLLAVDELRLETFGEGSCAQVLHLGPFADEGPTIE
                     RVHTFIDARGARRGKHHEIYLSDIRRADPAKWKTVIRQPMEPR"
     gene            complement(2793..3194)
                     /locus_tag="VM692_16325"
     CDS             complement(2793..3194)
                     /locus_tag="VM692_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HUQ53797.1"
                     /translation="MPALLTFTGAMEWDPAIAAYFDGRPAELVAIARPWFAEMRGCGP
                     DVRELFHDGCAVVCVEDAPFAYVNVLKAHVNVGFFHGDALRDPQQLLQGTGRFMRHVK
                     LEPKSPVAEQALSALIAAAYRDIKARLKGAR"
     gene            3388..4212
                     /locus_tag="VM692_16330"
     CDS             3388..4212
                     /locus_tag="VM692_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53798.1"
                     /translation="MRLQRRYLLALATVATALASGPLAAQWLDNKTPGVPRKADGTVD
                     MAAPAPRLPDGKPDFSGIWISAKQDQDLASDGHDLGSGRHMANLGIDMEGGLPYQPWL
                     VPIVKERTANLAIDDPHIRCLPDAFLRAYGLPHMLKFVHKPDLLVVLNEMNAGYRQVF
                     VDGRPLPDSPSPAWQGYSSAKWVGDTLVVDTIGFRDDNWIDWNGSVLTEAAKVREEIR
                     RPDFGHLDIKVTVDDPKAYTKPWTVQLRQRIVVDAELIDEVCLENEQFVQRMKLPN"
     gene            4222..4632
                     /locus_tag="VM692_16335"
     CDS             4222..4632
                     /locus_tag="VM692_16335"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53799.1"
                     /translation="MKTIIRAALLLSCASALTAHAHHSFPAQYDAAKTITLTGKVTKV
                     EWTNPHIFIYIDVPNETTGAVVNWALEMGGPNALLRLGWKRDSLKPDDLITVEGSLAR
                     DGTPLANAKSITMVATGKKMLAGSSADNGGLGSR"
     gene            complement(4636..4776)
                     /locus_tag="VM692_16340"
     CDS             complement(4636..4776)
                     /locus_tag="VM692_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53800.1"
                     /translation="MAEHQRLLSTLNLYGTGAWLVRAEGGALVQWHRFHRRCGPDWQC
                     LE"
BASE COUNT          900 a         1662 c         1578 g          838 t
ORIGIN      
        1 aagccgggca acgctactcg aaccatttcc ccgcctgtcg agcgaagacg ctgtcgagct
       61 ccaagggcgc caggtccggc tcgaccaagc cgaggcgcgc atcggccgac tccaagtgcg
      121 ggcgcgagta aaacttcagc agcgcaccgc gatcggcgag atcgctcgcg agcaaccgcg
      181 cgacacgctc ggcgacgggt gcgtcggcgt ggcgctcggc gaacgcggcg agcagctgca
      241 cgtacgccca cgttatcgtc tcgtgatagc cgctcgccgc cgaattgacg acgccgttgc
      301 tttcgttgag gcgccggatt ccgtcacgaa gtctcgcgag agcatcggcg gcgccgtagc
      361 ggctcacgtg ccaaagaccc accgacaagt gcgccgcgtg cgtccactcc cgcctcggca
      421 cctcgcaggc atggaaacgc cgcacgagat cttcgagctc agcgcctgtc atcgagacct
      481 acgccgctcg gccgtcgaag tggttgacgg ggatcgattc gaggtcgatg ccttcgaggc
      541 agcggaggtt gatggcggcc ttctgattgc ccttcgagtc cgtgccttcg ccgtacgggt
      601 gcatgccgca gttcggacag aaccgatgct tgatcgcgcg cttgttgaac gtataggtgt
      661 tcgcgttgcc ttccggtgtg agcagcttga ggtcctcgcg cggcacgaac caaagcaacg
      721 agcccttgcg ttggcagatc gagcaattgc atgcgagggc gctatcgatc gtgccctcga
      781 cctcgaaggc cactcgtccg cagtgacaac taccttggta tttcatcgcc gtgttcctcc
      841 tgtgttcgcg ctcaatcgcg acaataacct tcgccgggcc gcaccgtcag gcaattcgac
      901 ttctcggcga gccacttcat gcccgtggcg gcgccgtcgg tcgcatggat cgattcgatg
      961 acgccatcgc gctcgaaacg aatctggtcc cccatgctgc tgcccacgaa tacgcgcggc
     1021 ccgtcgagat tctgaatcgt cacgtcgtag cggccgttgc cgccttcgag caagaggaac
     1081 gtgccttcgg gccccgtcca ccggccgagc cagccgtcgg taatcgccgg cgaggtacga
     1141 ctggcatcgg ggcgcgagca gccggcgacc acgatcagcg cgagcgtgac ggctcggtag
     1201 cggcacctca tgcggacgcc gcctgtacgg gagcgcgact gcgcagctgt gtcagcatgt
     1261 cctcgacgac gtcatgggac gaccggtctt gcgtcgcgac gtggaccgcg tactgcggcc
     1321 gctgatgctc ggtgcaacat tcggccgctc tgcgaagctg ccacggcgtc ggtccgcgac
     1381 ggccctcgcg ttgctgcaat cggtgttgca cgaccgcgag cggcgccgtg aggcaaaagt
     1441 gcaacgtgtc gacatcgaag ctgcgaatgc cgtcgacgac ttcgcctaga tacgcttcgt
     1501 tcgagaacgc catcggaacg acgaccgtcg gccgcagcaa gcgagccaag cgaatcaggc
     1561 gaacgctgcc ggcacgccac gcacgtaggt cttggaagtc gtcgatgcgt ttgaacggtc
     1621 gtgcgagccg ctgcagcacc aggccgtgca gctccgggtc gaaaagcacg ctgcccggca
     1681 accgcgcgac gagccctcga gcgacggtcg tcttgccgat accgaaagac ccgttgatga
     1741 agatgaccat ggcgagacgc tcgctagacg ttcacgagca gattggcgtc gcgcgagcag
     1801 catgacggcc gcgggccgcg cggcggatgc gaagccgcgg cgaacgccgc tttgttcatc
     1861 ggcggccgcg cggcgaccag gaacaaggcg tcgtccgtca tgagctcgag cacggcgtcc
     1921 gtgtcgccgc gcttcgtggc cgcgagccac gtcgcgacga gcgcgcggat tgctcgctcg
     1981 tcagcgctca tggcttcgca gcccgtgacg gccgacggga ctggtctcga agcgctcgcc
     2041 gaatcccgcg atcagcgcgt cgcgctgccc gggcaccccc ttcatcttgc ctatcaggcc
     2101 gtacacggtt gccctcctct ccttgttaga ggcgccgaag catcatccgt agatgatacg
     2161 tgcgtcgccc gtcacctcgg ctccatcggc tggcgaatca ccgttttcca cttcgcggga
     2221 tccgcacggc gaatgtcgct caagtagatc tcgtggtgct tgccgcgccg cgcgccgcgc
     2281 gcgtcgatga acgtgtgcac gcgctcgatc gtcgggcctt cgtcggcaaa cggcccgagg
     2341 tgcagcactt gcgcgcacga cccctcacca aacgtctcga gccgtagctc gtcgacagcc
     2401 aggaggcctt tcttcttgcg aacctcggcg agggccgcct cgatgacggc gctcgtcacg
     2461 aacgacggct gcatgatcat catggtccac tgccatttgg acttgtcgcc ggctacgaat
     2521 accgagagat cgtcggccca ccaaagaccc tcgagcggca tcacgccgaa gtcgatggcc
     2581 tgcgctttct tgaccatgaa cttcgcggtg tacgacacgg cgaacagcgc ttcgacggcc
     2641 gccgcgtaag ccgtcgacgt gttcggatcg ccttcgccgt cgatcatcag aaactcgaac
     2701 gccggcacgt cgaccgccac gactgctttg acgctcggct gatagaggtg cttgagctcc
     2761 ttcttgaggt cgactttgcg catgtgcggc tctcagcgcg cgcccttgag ccgcgctttg
     2821 atgtcgcgat acgccgcggc gatcaacgcg ctcagcgcct gctccgccac gggcgacttc
     2881 ggctcgagct tcacgtgccg catgaagcgg cccgtccctt gcagcagctg ctgcggatcg
     2941 cgcagcgcgt cgccgtggaa gaagccgacg ttgacgtgcg ccttcaagac gttgacgtac
     3001 gcgaacggcg cgtcctcgac gcacacgacg gcgcaaccgt cgtggaacag ctcgcggacg
     3061 tcgggcccgc agccgcgcat ctccgcgaac cagggccggg cgatcgcgac gagctcggcg
     3121 ggccggccgt cgaagtaggc cgcgatcgcg ggatcccact ccattgcgcc ggtgaacgtc
     3181 agcaacgcgg gcacgatgac tctccgtggc tcttcagggg aggtgcagca tatgcgccgg
     3241 caccgtcagg aaccgtcgta tttgtgccag tgctatagct ccgcgcaata gcgagcgatc
     3301 cgaagatttc agggtggggg tcgacttcga ggcgccattt gcggtcgaat ctgctcctcg
     3361 agaggatggt cccgaaggga aaccacaatg cgattgcagc gccgctatct attggcactc
     3421 gccaccgtcg cgacggcgtt ggcttcgggc ccgttggcgg cccaatggct cgataacaag
     3481 acgccgggcg tgccgcgcaa ggccgacggc accgtggaca tggcggcgcc tgcgccgcgg
     3541 ctgccggacg gcaagccgga cttttcgggc atctggatca gcgcgaagca ggaccaggac
     3601 ctcgccagcg acggccacga cttgggctcg ggccgccaca tggcgaacct cggcatcgac
     3661 atggagggcg gcttgcccta tcaaccgtgg ctcgtgccga tcgtcaaaga gcgcacggcg
     3721 aacctcgcta tcgacgaccc ccacatccgc tgcctacccg acgccttcct gcgcgcgtac
     3781 gggttgccgc acatgctgaa gttcgtgcac aagccggacc tgctcgtcgt tctcaacgag
     3841 atgaacgccg gttatcgcca ggtgttcgtg gacggccggc cgctacccga ctcgccgagc
     3901 cccgcgtggc aaggctactc gtcggcgaag tgggtcggcg atacgctcgt cgtcgacacg
     3961 atcggcttcc gcgacgacaa ctggatcgat tggaacggca gcgtgctgac ggaagccgcc
     4021 aaagtgcgcg aggaaattcg ccgccccgac ttcggccatc tcgacatcaa ggtcaccgtc
     4081 gacgatccga aggcgtatac gaagccgtgg acggtgcagc tgcgacagcg cattgtcgtc
     4141 gacgccgagc tcatcgacga ggtctgcctg gagaacgagc aattcgtcca gcggatgaag
     4201 ctaccgaact gaggacacga catgaagacg atcatccgag cggcattgct tctttcgtgc
     4261 gcgagcgccc tcacggcaca cgcgcatcac tcgttccccg cgcagtacga cgccgcaaaa
     4321 acaatcacgt tgaccggcaa ggtcacgaag gtcgagtgga cgaacccgca catcttcatc
     4381 tacatcgacg tgccgaacga gacgacgggc gccgtcgtca attgggcgct cgagatgggc
     4441 ggcccgaacg cgttgctgcg cttgggctgg aagcgcgact cgctgaagcc cgacgacctc
     4501 atcaccgtcg aaggcagtct cgcgcgcgac ggcaccccgc tcgcgaacgc gaagtcgatc
     4561 acgatggtcg ccacaggcaa gaaaatgctc gccggctcga gcgctgacaa cggcggcctc
     4621 ggctcgcgct agccgtcact cgaggcactg ccagtccggt ccgcagcggc gatggaagcg
     4681 gtgccactgc acgagcgctc cgccctcggc ccggaccaac cacgctccgg ttccgtagag
     4741 gttcagcgtg ctcaaaaggc gttgatgctc ggccatcgag gcgctgcgct ccggttccct
     4801 gtactgcgaa tcggacggga acagcagcag gacgatgcat aaactacgtc gacactcgac
     4861 gtaggactcc gtgaagcccc agcttggact agtcgtccaa aggcttcgac aagaaccgtg
     4921 tcaatgtcgg cttcgaaatg acgaagtcct cgaacccgaa gctctgatat agagcgcg
//
LOCUS       DATLEE010000745         3688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6495933, whole genome shotgun sequence.
ACCESSION   DATLEE010000745 DATLEE010000000
VERSION     DATLEE010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6495933"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VM692_16345"
     CDS             <1..873
                     /locus_tag="VM692_16345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUQ53801.1"
                     /translation="QRVVKDVLRNPTSADGDWLTRDGRRIALAASSLPQESVKIILLT
                     DVTDTRALQELAARNQRLSAIGKMAASLAHQIRTPLAGALLYLSQCRSRRDERERGKL
                     LEKGIERLRHLDLLVQDMLVFARGKGPGERVRVADLFKAVHDAAMAVKPPNAYLVIHG
                     SDALVELDGNRTALTAALTNLVNNAFEAAPDVIVTLQAKLRGDRVEFTVRDNGPGIAP
                     SIQSRVFEPFFSTRPAGTGLGLAVVKTVAEAHGGDLALHSEIDRGTRIEINLPRVAPA
                     ATPAPAATAAREVA"
     gene            870..2237
                     /locus_tag="VM692_16350"
     CDS             870..2237
                     /locus_tag="VM692_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUQ53802.1"
                     /translation="MSSGTILVVEDDRPLQDALVTTLEAAGFNVLAAGDGGEALKVLG
                     ASSVDLIVSDVEMRPVDGRELLRRLQQRESAPPVLLMTAYGTIDQAVAAMRDGAVDYL
                     VKPFEAEELEQRVARYVRAGGAAVRDAAAAAPPVADDPVSRKLLELAARVAQSDVTVL
                     LNGESGTGKEVLARYIHARSKRSTGPFVAVNCAAIPEQMLEALLFGHEKGAFTGAQAR
                     SVGKFVQANGGTLLLDEVTEMELGLQAKLLRVLQEREVEPLGASKPLPLDVRVLATSN
                     RDLEQAVRDGAFREDLYYRLSVFPLRIAPLRERPGDIDALTMRCIAARSPRPLTISPA
                     AIVRLQSYTWPGNVRELENVVQRAILMADGAPRIEPEHLVLGDNRGTAPQAPAAAATG
                     LAGELWEEETRRIVAALETHRGVRRQAAEQLGISDRTLRYKLSKIRAAGIQVPGDRVA
                     VGESR"
     gene            2234..2548
                     /gene="fliE"
                     /locus_tag="VM692_16355"
     CDS             2234..2548
                     /gene="fliE"
                     /locus_tag="VM692_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HUQ53803.1"
                     /translation="MSGVEMTRLLGEMQRLAATADARPSEATHESSGFAELLKSSIDA
                     VNDAQASASDMAAALERGDKSVTLPEVMIALQKASLSFQAMTEVRNRLVNAYQEIMNM
                     PI"
     gene            2568..>3688
                     /gene="fliF"
                     /locus_tag="VM692_16360"
     CDS             2568..>3688
                     /gene="fliF"
                     /locus_tag="VM692_16360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HUQ53804.1"
                     /translation="MADTESTANAAAGGLSLPLYALLPPAVKQILLLVGLAAAVAAGI
                     AMVIWSQGENYTQLYSGLADRDVGEITAQLDSANVPYELDPGTGSLLVPADRKYEVRM
                     QLASSGLPRGAGFGIEEMPDRSSFGQTPFMENALYVRAVETELARTIGSMQPVEMARV
                     HLALPPQSVFLRQKREPSASVMLKLFNGRRLDEEQVQSVVHLVASSVPDLVPSRVTVV
                     DQTGALLTSQSDDSTTSLTGSQFDYRKQVEADSSQRIQSLLGSIVGADRVRASVSAEI
                     DFTQVEQTRESFDPNVQMVRSEQTSEDTRRGDGLQGVPGALSNQPPEVAAPPAGVNGA
                     TTAEATSTSRSQTRNFELDKTVSHTRAAVGSISRLSIGVL"
BASE COUNT          614 a         1260 c         1274 g          540 t
ORIGIN      
        1 cagcgcgtcg tcaaagacgt cctgcgcaac ccgacgtcgg ccgacggcga ttggctgacg
       61 cgcgacggca gacgcattgc gctcgctgcg agctcgttgc cgcaggaaag cgtcaagatc
      121 atcttgctga ccgacgttac cgacacgcga gcgctgcaag agttggcagc ccgcaaccag
      181 cgcttgagcg ccataggcaa gatggccgcg agcctcgcgc accagattcg cacgccgctc
      241 gcgggcgcat tgctgtacct gtcgcagtgc cgctcgcgcc gcgacgagcg cgagcgcggc
      301 aagttgctcg agaagggcat cgaacggctg cggcatctcg acctgctcgt gcaggacatg
      361 ctcgtgttcg cgcgcggcaa agggcccggc gagcgcgtac gcgtcgcgga cctgttcaag
      421 gccgtacatg acgcggcgat ggccgtgaag ccgccgaacg cgtatctcgt catccacggc
      481 agcgacgcgc tcgtcgagct cgacggcaat cgcacggcat tgacggctgc gctgacgaac
      541 ctcgtcaaca acgctttcga agctgcgccc gacgtcatcg tgacgctgca ggccaagctg
      601 cgcggcgacc gcgtcgagtt cacggtgcgg gacaacggcc ccggcatcgc gccgagcatt
      661 cagtcgcgcg tgttcgagcc gttcttctcg acgcggccgg ccggcacggg tctcggcctc
      721 gctgtcgtga agacggtagc agaagcgcac ggcggcgact tggcgctgca ttcggagatc
      781 gatcgcggca cgcgcatcga gatcaacctg ccgcgcgtcg cgcccgccgc aacgccggcc
      841 ccagctgcta ccgcggcacg ggaggtcgca tgagctccgg cacgattctc gtcgtcgagg
      901 acgaccggcc gttgcaagac gcgttggtca cgacgctcga ggcagccggc ttcaacgtgc
      961 tcgcggccgg cgacggcggc gaagcgctga aggtgctcgg cgcgagctcc gtcgatctga
     1021 tcgtcagcga cgtcgagatg cggcccgtcg atggccgcga gctgctgagg cgcttgcaac
     1081 agcgcgagtc cgctccgccc gtgctgctga tgacggccta cggcacgatc gatcaagccg
     1141 tcgcggcgat gcgcgacggc gccgtcgact atctcgtcaa accgttcgag gccgaggagc
     1201 tcgagcaacg cgtcgcacgc tacgtgcgcg ccggcggcgc agccgtgcgc gacgcggcgg
     1261 cggcggcccc gcccgtcgcc gacgatcccg tgagccgcaa gctgctcgag ctcgccgcgc
     1321 gcgtcgcgca gagcgacgtt acggtgctgt tgaacggcga aagcggcacg ggcaaggaag
     1381 tgctggctcg ctacattcac gctcggtcga agcgcagcac cgggccgttc gtcgccgtca
     1441 attgcgccgc gattccggag cagatgctcg aagcgctgct gttcggtcat gagaagggcg
     1501 ccttcacggg ggcgcaagcg cgcagcgtcg gcaagttcgt gcaagcgaac ggcggcacgc
     1561 tgctgctcga cgaggtcacg gagatggagc tcggcctgca ggccaagctc ttgcgcgtgt
     1621 tgcaagagcg cgaggtcgag ccgctcggcg ccagcaagcc gctgccctta gacgttcgcg
     1681 tgctcgcgac gagcaaccgc gatctcgagc aggccgtgcg cgacggcgcg tttcgcgagg
     1741 acctctacta tcgcctcagc gtttttccgc tgcgcatcgc gccgctacgc gagcgccccg
     1801 gcgacatcga cgcgttgacg atgcgctgca ttgcggctcg ctcgccgcgg ccgctgacga
     1861 tatcgccggc cgcgatcgtt cggctgcagt cctacacgtg gccgggcaac gtgcgcgagc
     1921 tcgagaacgt cgtgcagcgc gcgatcctaa tggccgacgg tgcgccgcgc atcgagccgg
     1981 agcatctcgt gctcggcgac aaccgcggca cggcgccgca ggctcctgcg gccgcagcga
     2041 cgggcctcgc gggcgagctc tgggaagagg agacgcggcg tatcgtcgcg gcgctcgaga
     2101 cgcatcgcgg cgtccgccgg caggccgccg agcagctcgg catcagcgac cgcacgctgc
     2161 gctacaagct gtcgaagatt cgtgccgcgg gcattcaagt gcccggcgac cgcgtcgcgg
     2221 tcggggaatc acgatgagcg gcgtcgagat gacgcgtctg ctcggcgaga tgcagcgcct
     2281 cgcggcgact gccgacgcgc gcccgagcga ggcgacgcac gaatcgagcg ggttcgcgga
     2341 gctgctgaag tcgtcgatcg acgccgtcaa cgacgcgcag gcctcggcgt ccgacatggc
     2401 ggccgcgctc gagcgcggcg acaagtccgt gacgttgccc gaggtgatga tcgcgctgca
     2461 gaaggcgagc ctgtcgttcc aggcgatgac cgaggtgcgt aaccgcctcg tcaacgccta
     2521 ccaagaaatc atgaacatgc cgatctaggt cgaggttcca gaaacacatg gccgataccg
     2581 aaagtaccgc caacgccgcc gccggcggcc tcagtttgcc gctgtatgcg ttgctgcccc
     2641 cggcagtgaa gcaaatccta ctgctcgtcg gcctcgcggc cgccgtcgca gcgggcatcg
     2701 cgatggtgat ctggtcgcag ggcgagaact atacgcagct ctactcgggc ctcgcggacc
     2761 gcgacgtcgg cgagatcacg gcgcagctcg acagcgcgaa cgtgccgtac gagctcgatc
     2821 ccggcacggg cagcttgctc gtcccggccg atcgcaagta cgaggtgcgc atgcagctcg
     2881 cgtcgtcggg tttgccgcgc ggcgccgggt tcggcatcga ggagatgccg gaccgcagct
     2941 cgttcggcca gacaccgttc atggagaacg cgctgtacgt gcgcgccgtc gaaacggagc
     3001 tcgcgcgcac gatcggcagc atgcagcccg tcgagatggc gcgcgtgcat ctcgcgctgc
     3061 cgccgcagtc ggtgttcttg cgccaaaaac gcgagccgag cgcgtcggtc atgctgaagc
     3121 tgttcaacgg tcggcgcctc gacgaagagc aagttcaatc cgtcgtgcat ctcgtcgcgt
     3181 cgagcgtccc ggacctcgtc ccgtcgcgcg taaccgtcgt cgatcagact ggcgccttgt
     3241 tgacgagcca gagcgacgac tcgacgacga gcctcacggg ctcgcagttc gattaccgca
     3301 agcaggtcga ggccgactcc tcgcagcgca tccagagcct gctcggctcg atcgtcggcg
     3361 ccgaccgcgt gcgggccagc gtctcggcgg agatcgattt cacgcaggtc gagcagacgc
     3421 gcgagagctt cgacccgaac gtgcagatgg tccgcagcga gcagaccagc gaggacacgc
     3481 ggcgcggcga cggcctgcag ggtgtacccg gcgcgctgag caatcagccg cccgaggtgg
     3541 cagcgccgcc cgcgggcgtc aacggcgcca cgaccgcaga agccacgagc acctcgcgct
     3601 cgcagacgcg caatttcgag ctcgacaaga cggtcagcca cacacgcgcg gccgtcggct
     3661 cgatcagccg cttgtcgatc ggcgtgct
//
LOCUS       DATLEE010000746        11827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3710755, whole genome shotgun sequence.
ACCESSION   DATLEE010000746 DATLEE010000000
VERSION     DATLEE010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11827
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3710755"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VM692_16365"
     CDS             complement(<1..537)
                     /locus_tag="VM692_16365"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUQ53805.1"
                     /translation="MQHRTPRKKREPRRPGRPRADETNQRERLLDAAVACFAADGVAA
                     TSLRSIALKAGVTPALVNYYFGSKELLLDAFIAERVTPAVSVLRESLLEAGDDPRALL
                     AAFVRGIHGVVERFPWWPALWVREVLNEKGALGDVLHKIIAPQVTQLLARTLVDAQKR
                     GALSRDLDPRLLVVSLVGL"
     gene            687..1313
                     /locus_tag="VM692_16370"
     CDS             687..1313
                     /locus_tag="VM692_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53806.1"
                     /translation="MASAVALGAGVLAQSAHAQRSPVRVEREEPVIVRAEYDPRRPPA
                     DMPELKPPESGVCKTTFELSASVTYSAERLSRTSARIYVDELDIVTRLRFDIFTIKNA
                     PAKLRAHEEGHRAIGEHYYESAERIATDIGKRLIGATFDGTGADQDAAQQDGFNKVVE
                     EIERAYMALVRAPSASANERFDEITNHGLDPIDENEAIRRAIAGTPPA"
     gene            1361..2698
                     /locus_tag="VM692_16375"
                     /pseudo
     CDS             1361..2698
                     /locus_tag="VM692_16375"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
     gene            2787..4046
                     /locus_tag="VM692_16380"
     CDS             2787..4046
                     /locus_tag="VM692_16380"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HUQ53807.1"
                     /translation="MSLAWISVAALVLAITLSCTTTINVGVLSLALALIVGVGLGNMS
                     PGDVLDGFPVDLFTTLVGVTLLFAIADVNGTLARLTERAVRLCRGHAGALPLMFFAIG
                     FVVATIGAGATPASALLAPPAMAVAARAGVPPLLRASMAGNGALAGTLSPFAPTGVVA
                     HQNMEQIGLGGYEWVTFTYNALAHTLVGFGGFFLLGGWKLFRRGAARADAADVETVEP
                     MNVQHWTTTAGIVALILAVVGFDAAGFDVNVGMVALIVVTVLVLLRTVDEAKAIARMP
                     WGVILMVTGVTVLIALLRDTQGLQLIASGIARVSTPETIEPVVAFGVGIVSVYSSTSG
                     VVLPAFLPMVPDLAAQLGGHELLPIAWSMNVSASLVDLSSLSTGGALFIAGAAGFADT
                     RKLFNQLLAWGMSMAVVGAVLCYFLFG"
     gene            4126..6648
                     /locus_tag="VM692_16385"
     CDS             4126..6648
                     /locus_tag="VM692_16385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HUQ53808.1"
                     /translation="MARSEQLNRARWFLGLAQDLRTALRLFASTPLVSAVAVLSLGLG
                     IGATTATFSLINSVYLRPLPVDSPEELVSITGPTRSGRAGRQVAELLRDAPMFERLAA
                     YTETQFDVSPDNEARWVDGLWVGGTFFETMGVAPFAGRLLSEADDIAGGGADGPVAVI
                     SHSLWQRRFASAPDALGRSIDVNHTPFTIVGVAPPDFFGPNVGRSFDVALPLSAAGLL
                     IGDGGAQPGDDVIARLRTGQTLAAANAALQTWLTNQVPADRAGFVSQVRAVPAATGLS
                     PVRDEYRQPLAMVLALGALVLLVAAANVANLMLAWATVRRHELSVRRALGASSWRLVR
                     QCSVESFLLAAVSAMAGVLVAVVAARLLVRGLAARDALTTGDIAFGSARLVLDAGLDW
                     RVWAFTTGLAVLTALLFGIAPALRSLAAAPVDALKESTALTAQGGRSWRFGAVRLGVS
                     GASGILVAQVALSLMLVVSAGLFVRSFSLLSNVDIGIDAARVLLVNIRRAGRTEEPMP
                     HERILDSVRGLPSVAAAGVSLGTPPISSGAYEAFTVVGADPPENAEGTANLVTPEWFA
                     ALGMRTVAGRLLTGGDVAGAPRVAVVNEAFATTFVGTSSAIGRTISSFERPIEIVGVV
                     GNVISGALGDRPQPAVYLPLAQADGRIRDLYYFFDADRGLLLTVRSATGSPTALTRAL
                     GDAVQRVEPGAVLTFRPLDEQLAALTARERVLAMLSTFFGVLALLLAALGLYGLVAYT
                     VARQRREMAIRLALGAPPAGVVAGVLGRVLALIGIGTTFGAALSAWSGKFLAALLYGV
                     QPTDVSVFLGAALILTIGGAAVAWLPARHAARIDPATALRYE"
     gene            complement(6639..7706)
                     /locus_tag="VM692_16390"
     CDS             complement(6639..7706)
                     /locus_tag="VM692_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017691556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional activator/DNA
                     repair protein Ada/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HUQ53809.1"
                     /translation="MDTTQLPLDQDRCYTALLGRDADYEGVFYVGVRTTGIFCRPTCP
                     ARKPKRENCEFFTDAQSALLAAYRPCARCRPLSHPNETSDVVRKLVAAVESDPTKRWR
                     DADFDALAVHASTARRQFQKRFGMTFVEYARARRLGSAFKSIRAGERVIDAQLDAGFE
                     SSSGFRDAFARIMGAPPARSTRALFAAWLDTPLGPMTAIADEEALYLLEFVDRRGLER
                     EIERLRLRHKAGIAPGRTAPIIQIEQELADYFAGRSTTFETPIARSGSPFQNAVWDAL
                     LTIPPGETWSYAQLARVVGRPKAVRAAGTANGCNQLAIVIPCHRVITSAGELGGYAGG
                     VPRKRWLLEHERRMRAATRHS"
     gene            complement(7757..8182)
                     /locus_tag="VM692_16395"
     CDS             complement(7757..8182)
                     /locus_tag="VM692_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HUQ53810.1"
                     /translation="MARPRTKCRPRRSLRVSPAFREFVLDQLARVPQLRPKAMFGGVG
                     LYSGERFFGILAADELFFKVDDRNRAAYEAAGSEPFRPVADRPVSMSYFRVPIEVLED
                     PTELAEWARGAIRAAATPKPKAQKPRRRPSKTPQEPGRN"
     gene            complement(8185..9366)
                     /locus_tag="VM692_16400"
     CDS             complement(8185..9366)
                     /locus_tag="VM692_16400"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUQ53811.1"
                     /translation="MNRRELIFAAASAALFSTDRVAAQPRRYDLIIKGGRVIDPSLRL
                     DATRDVGIAGGRIAAVEPTLAAEAAATLDARGKLVVPGLIDIHTHAGRDMAGPSMLLR
                     DGVTGWIDAGSAGADGIDAVVAVARASQQPGRVLVNIGRGGILPDGDTKDLKLADAAA
                     ARAAIEKHRDYVIGIKARLSETVVGKNDVEVLKRAQQVVEPLGLPVMIHMGQSPSPLR
                     ELLPILKTGDIVTHLFAPPPNAIVDNNGKLLPEVLAARRRGIVFDVGNGVRDHMRWDT
                     IEQIMGVGFWPDTISTDWNVMSGTTGVVDFPNCMSKLLGFGMSVADVVACATFNAART
                     FAVFRDRGTLNVGAPADVAVLELREGEFEFVDNYDNKLKGRQRLFPGETVLGGVRVPR
                     A"
     gene            9516..9908
                     /locus_tag="VM692_16405"
     CDS             9516..9908
                     /locus_tag="VM692_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53812.1"
                     /translation="MRTLALALVATAVAGSSYAADEPVQPTSSYFEGAKITVNARAKA
                     DGFMRVRVVPENGEPREATFGIAKRMNENDLAKGIAVSLNTVLLPEYVADKDNGEHVK
                     IQKKQKNAPNFSVEITFNAPGFSVILDD"
     gene            complement(9943..10989)
                     /locus_tag="VM692_16410"
     CDS             complement(9943..10989)
                     /locus_tag="VM692_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53813.1"
                     /translation="MPIEDELQDRRNDYDVTNTVQVSSSPQVRSAVADLFGALYPNSS
                     FDSVWLAFHDFERVFRGLDPSYHAVDTTYHDMQHTLDMTLALARLIAGHELSVEPGDR
                     LGPDRAELAIVSALFHDVGYLRHRDRDAAAVNGAVFTRVHVTRSGQYLESYLPRIGLE
                     QFAPVVSRIVHFTGYELNVDQIELEEPKDSVVGHLLGTADLVAQLADRCYLEKCRDRL
                     YPEFVLGGVAIDERPQGKVLYHSAQDLLGKTLSFYQTSARLRLENNFNRVYRYFEAFF
                     ERGQSPYIRFIRKNLTFLNTVIQNGDWERLRRHPPCIMPDPQGEAHLIELALQRVRDW
                     SSTRPKVPPLAVAG"
     gene            complement(11062..>11827)
                     /gene="xth"
                     /locus_tag="VM692_16415"
     CDS             complement(11062..>11827)
                     /gene="xth"
                     /locus_tag="VM692_16415"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HUQ53814.1"
                     /translation="ATWNVNSLRVRLEHVLKWLERERPDVLALQETKLKDEDFPADEF
                     RDLGYVATFSGQAAYNGVAILSREPPSDVAYGIGDHYADEQKRVLAATFGSLRLLSLY
                     VPNGQSVGSDKYGYKLEWLAALRVSLEAELARHASLLLVGDFNIAPDDRDVHNPAAWQ
                     GKIMCSPGERAALAAICSLGLSDVFRLFSQPEKVFSWWDYRAGAFRRNQGLRIDLMLA
                     SRALSQTCSACRIDKEPRGWERPSDHVPVVAEFTPS"
BASE COUNT         1819 a         3935 c         4102 g         1971 t
ORIGIN      
        1 gaggccaacc agcgacacga cgagcaggcg cgggtcgaga tcgcggctca gcgcgccgcg
       61 cttctgcgcg tcgacgagcg tacgcgccag cagctgcgtg acctgcggcg cgatgatctt
      121 gtgcagcacg tcgccaagcg cgcctttttc gttcaagacc tcgcgcaccc agagcgctgg
      181 ccaccacggg aagcgctcga cgacaccgtg aatgccgcgc acgaacgccg cgagcagggc
      241 gcgcggatcg tcgccggctt cgagcaggct ctcgcgcagt acggagacgg cgggtgtcac
      301 gcgctcggcg atgaatgcat cgagcaacag ctccttgctg ccgaagtagt agttgacgag
      361 cgccggcgtg acgccggcct tgagcgcaat gctgcgcagg ctcgtggccg caacgccgtc
      421 agccgcgaag caggcgacgg ccgcgtcgag cagccgctcg cgctgattcg tctcgtcggc
      481 gcgcggccgg cccgggcgac gcggctcgcg tttttttcgt ggcgtgcgat gctgcatcgg
      541 cctaaattaa attagcgttt aaataattac aagctgggca agcgatgtgc tagtctttcg
      601 gccgcttttc ctcacccgac ggccgcctaa gcgactgttc ccgcgcgaaa cgtatgactc
      661 agggctccat ggatcggcgc accttcatgg cttcagccgt cgcgctcggg gccggcgtgc
      721 ttgcccagag cgcgcatgcg caacgctcgc cggtgcgcgt agagcgcgag gagcccgtca
      781 tcgtgcgcgc ggagtacgac ccgcgccggc cgccggccga catgccggag ctgaagccgc
      841 cggagtccgg cgtctgcaag acgacgttcg agctgtctgc gagcgtgacg tattcagccg
      901 agcgactgtc gcgcacctcg gcgcgcatct acgtcgacga gctcgacatc gtcacgcgct
      961 tgcgcttcga catcttcacg atcaagaacg cgccggcgaa gctgcgcgcg cacgaagaag
     1021 ggcatcgcgc gatcggcgag cattactacg agagcgccga gcgaatcgcg acggacatcg
     1081 gcaagcggct gatcggcgcg acgttcgacg gtaccggcgc cgaccaggac gccgcgcagc
     1141 aagacggctt caacaaggtc gtcgaggaaa tcgagcgcgc ttacatggct ctcgtgcgcg
     1201 ccccgagcgc ctcggccaac gagcgcttcg acgagatcac caaccacggc ctcgatccga
     1261 tcgacgaaaa tgaagcaatc cggagggcca tcgcgggaac cccaccggct tgagcttaag
     1321 ttggcagtca cgctagtcgg ccggctatgc ttgccgtcct atgacaacaa caaagacgac
     1381 cacgggcgcg gtcgtcgact tcatccaaag cgccaagatc gccgatttcc ccgcagacgc
     1441 gctgactacg gctaaacgct gcatcatcga tagcctcgca gtcatgctcg cgggctcgac
     1501 gcaggacgcg gggcgcattc tgcatgagca tgtgaagggc tcggacccgc gcacggccgc
     1561 cacggtgttc gggccgaagc cgttcaagac gggctcggcc tcggccgcgc tcgtcaacgg
     1621 cacggccggc cacgcgctcg actgggacga cacgcagctc gcgacgagcg cggatcgaat
     1681 cttcggcctg ctgacgcacc ccagcatgcc gccgctcgtc gcaacgctcg ccgtcggcga
     1741 gccgaagcgc gtcagcggca agcagttcct cgaggcgttc ctgacgggct tcgaggtcga
     1801 gtgcaagatc gccgaggcga ttcatccgaa ccactacaag aagggctttc acacgtcggg
     1861 cacgatcggt acgttcggcg cggcctcggc cgcgacgaag ctggtcggcc tcggcgcgga
     1921 gcagacggcg cacatgctgg cgatcgccgc gagctcggcg tccggcatcc gcgtgaattt
     1981 cggcagcatg acgaagccgc tgcacgtcgg ccgcgcggct caaaacggcg tgacggcggc
     2041 cgagctcgcc gcgcgcggct tcacgggcgg caaggacgcg ctcgatccgc cgtgggggtt
     2101 cttcgcgacc ttcgggcacg gcgaagccta cgacccggcg cgcatcgttc ctctgatggg
     2161 caagccgcac acgatcgtgt cgccgggcgt ctcgatcaaa ccgtatccgt gcggggtgct
     2221 tggtcatccg acgatggacg caatgcgtcg gctcgtcatg aagcacgacg tcaagccggt
     2281 tgagatcaag gcgattcggg tccgcgcggg ctcgaatatt ctgaatcctt tgcgctatcc
     2341 gattgcgagc aacgagctcg aagcgaagtt ctgcccggcc ttcatggtca gcgcgatcgc
     2401 gctgcgccgc aaagccggca tccgcgagtt caacgacgag ttcgtgcaga gcgcgcccgt
     2461 gcaagcgctg atgcgcaagg tcgagcgcgt gctcgatccc gagatcgagg ccaaaggctt
     2521 cgagaagatc cgcagcacgg tcgaggtcga tctcgtcgac ggccgcaagc tcgtcgagca
     2581 cgccgacgag cgctatcgcg gcgggcccga cctaccgttc acgcgcgacg agctctacga
     2641 gaagtggagc gattgcgcgt cgctcgtgct gccgcaagcg ggcgttgaca aggtgttcga
     2701 gctcgtcgac aagctcgaac agttgcccga cgtcacggcg ctcggccgcg cggtgaccgc
     2761 gtccgcgaac gacgaggcgc agcccgatga gcctcgcgtg gatttccgtc gcggcgctcg
     2821 ttctggccat tacgctgtcg tgcaccacga cgatcaacgt cggcgtgctg tcgctcgcgc
     2881 tcgcgctgat cgtcggcgtc ggcttgggaa acatgtcgcc cggcgacgtg ctcgacggct
     2941 tccccgtcga cctcttcacg acgctcgtcg gcgtgacgct gctgttcgcg atcgccgacg
     3001 tcaacggcac gctcgcgcgg ctcacggaac gggccgtgcg gctctgccgc gggcacgcag
     3061 gcgcgctgcc gttgatgttc ttcgcgatcg gcttcgtcgt cgcgacgatc ggcgccggcg
     3121 cgactcccgc gagcgcgctg ctcgcgccgc cggcgatggc cgtggccgcg cgcgccggcg
     3181 tgccgccgct gttgagggca agcatggccg gcaacggcgc cctcgcgggc acgctgtcgc
     3241 cgtttgcgcc aacgggcgtc gtggcgcatc agaacatgga gcagatcggt ctcggcggct
     3301 acgagtgggt gacgttcacc tataacgcgc tcgcgcacac gctcgtcggg ttcggcggtt
     3361 tcttcctgct cggcggttgg aagctatttc gccgcggcgc ggcgcgcgcc gatgcggccg
     3421 acgtcgagac cgtcgagccg atgaacgtgc agcattggac cacgacggcc ggcatcgtcg
     3481 cgctgatcct cgccgtcgtg ggcttcgacg ccgcgggctt cgacgtcaac gtcggcatgg
     3541 tcgcgttgat cgtcgtgacg gttctcgtgc tgctgcgcac cgtcgacgaa gcgaaagcga
     3601 tcgcgcgcat gccgtggggc gtcatcctca tggtcacggg cgtgaccgtg ctgatcgccc
     3661 tgttgcgcga cacgcagggt ttgcagctga tcgcgtcggg gatcgcgcgc gtgtcgacgc
     3721 cggagacgat cgagcccgtc gtcgcgttcg gcgtcggcat cgtctcggtc tacagcagca
     3781 cctcgggcgt cgtgctgccc gcgttcctgc cgatggtgcc cgatctcgcc gcgcagctcg
     3841 gcggccacga gctgctgccg atcgcctggt cgatgaacgt gtcggcgagc ctcgtggatc
     3901 tgtcgtcgct gtcgacgggc ggcgcgctgt tcattgccgg cgccgccggc ttcgccgaca
     3961 cgcgcaagct gttcaaccag ctgctggcgt ggggtatgtc gatggccgtc gtcggcgccg
     4021 tgctctgcta ttttttgttc ggctgacggt cgagcgaccg cggcggacgc cgaaccggga
     4081 accttttgcg cctcaggtgc gaaagaatga tggggggacc aaccgatggc tcgtagtgag
     4141 caactgaatc gtgcgcgctg gttcttgggt ctagcgcagg atctgcgcac agcactgcgc
     4201 ttgtttgcct cgacgccact cgtgtccgcg gttgccgtgc tgtcactcgg gctgggcatc
     4261 ggcgcgacca cggcgacgtt ctcgttgatc aatagcgtgt atctgcggcc gttgcccgtc
     4321 gacagccccg aggagctcgt ctcgatcacg ggcccgacgc gaagcgggcg cgcggggcgg
     4381 caagtcgccg agctgctgcg cgatgcgccg atgttcgaac gcctcgcggc ctacacggag
     4441 acgcagttcg acgtctcgcc cgacaacgag gcgcgctggg tcgacgggtt gtgggttggc
     4501 ggcacgttct tcgagacgat gggggtcgcg ccgttcgcgg gccggctgct gtccgaagcc
     4561 gacgacattg cgggtggcgg agccgacgga cccgtcgcgg tcatcagcca ctcgctgtgg
     4621 cagcggcgct tcgcgagtgc gcccgacgcg ctcggccgat cgatcgacgt gaatcacacg
     4681 ccgttcacga tcgtcggtgt ggcgccgccc gacttcttcg ggccgaatgt cggccggtcg
     4741 ttcgacgtcg cgctaccgct gagtgccgcc ggattgttga tcggtgacgg cggagctcag
     4801 cccggcgacg acgtcatcgc gcggctgcgc acgggacaga cgctggccgc cgcgaacgcg
     4861 gcattgcaga cgtggctcac gaatcaggtc cccgccgacc gcgcggggtt cgtgagccaa
     4921 gtgcgcgcgg tgccggccgc gaccggccta tccccggtcc gcgacgaata tcggcagccg
     4981 ctcgccatgg ttctcgcgct cggcgcgctc gtgctgctgg tcgcggcagc gaacgtggcc
     5041 aacctcatgc ttgcctgggc aacggtgcgg cggcacgagc tcagcgtgcg acgcgcactc
     5101 ggcgcttcgt cgtggcggct cgtgcgccag tgctccgtcg aaagttttct gctcgccgca
     5161 gtcagcgcca tggcgggcgt gctcgtcgct gtggtcgccg ctcgtttgct cgtgcgcggt
     5221 ctcgcggccc gcgacgcgct gacgacgggc gatatcgcct tcggcagtgc ccgcctggtg
     5281 ctggatgccg ggctcgattg gcgcgtctgg gcgttcacga cggggctcgc cgtgctcacg
     5341 gcgctgctat tcggtatcgc ccccgcactg cgctcgttgg cggccgctcc cgtcgacgcg
     5401 ctgaaggaga gcacggcact tacggcacag ggcggccgct cgtggcggtt cggcgccgtg
     5461 cgcctcggcg tatccggcgc ctccggcatt ctggtcgcgc aggtcgcgtt gtcgctgatg
     5521 ctcgtcgtca gcgcggggct gttcgtccgc agcttcagtc tgctgtcgaa cgtggatatc
     5581 ggtatcgacg ctgcgcgcgt gttgctcgtc aacatccgcc gcgcgggcag gaccgaagag
     5641 ccgatgccgc acgagcgaat cctcgacagc gtccgcggct tgccaagcgt cgcggccgca
     5701 ggcgtctcgc ttgggacgcc gccgatcagc tcgggcgctt acgaagcgtt cacggtcgtg
     5761 ggcgcggatc cgcccgaaaa cgccgagggc acggcgaacc tcgtcacacc cgagtggttc
     5821 gcggcgctcg gcatgcgcac ggtcgccggc cggttgctga ccggcgggga cgtcgccggc
     5881 gcgccgcggg tcgccgtcgt caacgaggcg ttcgccacga cgttcgtggg gacctcgagc
     5941 gccatcggcc ggacgatctc gtcgttcgag cggccgatcg aaatcgtagg cgtcgtgggc
     6001 aacgtgatct cgggcgcgct cggcgaccgg ccacagccgg ccgtctacct gcctctcgcc
     6061 caagccgacg gcaggatccg cgacctttac tattttttcg acgcggaccg cggcctgctg
     6121 ctgacggtgc gcagcgcgac cgggtcgccg accgcattga cgcgcgcgct cggtgacgcc
     6181 gtgcaacgcg tcgagcccgg cgccgtgctg acgttccgtc ccctcgacga gcagctcgca
     6241 gcgctgaccg cgcgcgagcg cgtgctggcg atgctgtcga cgttcttcgg cgtattggcg
     6301 ctgctgctcg cagccctcgg gctctacggc ctggtcgcgt acacggtcgc gcgacagcgc
     6361 cgcgagatgg ccattcgact cgcgctcggc gcgccgccgg ccggcgtggt cgccggagtt
     6421 ctcggccgcg tgctcgcgtt gatcggcatc ggaacgacgt tcggcgcggc gctgagtgcg
     6481 tggagcggca agttcctcgc ggcgctcctc tacggagtcc agccgaccga cgtctcggtg
     6541 ttcttgggcg ctgcgttgat tctcacgatc ggcggcgcgg ccgtggcgtg gttgcccgcg
     6601 cgccatgcag cgcgcatcga ccctgccacg gcgctgcgtt acgagtgacg cgtcgccgcg
     6661 cgcatgcggc gctcgtgctc gagcagccag cgcttgcgcg gcacgccgcc cgcgtagccg
     6721 ccgagctcgc cggccgaggt gatgacgcgg tggcacggaa tcacgatcgc cagctggttg
     6781 cagccgttgg ccgtccccgc cgcgcgcacg gccttcggcc tcccgacgac gcgcgcgagc
     6841 tgcgcgtagc tccaagtctc gccgggcggg atcgtcagca gcgcgtccca gacggcgttt
     6901 tgaaaaggcg agccgctgcg cgcgatcggc gtctcgaacg tcgtcgagcg gcccgcgaag
     6961 tagtcggcga gctcttgctc gatctgtatg atcggcgcgg tccgccccgg cgcgataccg
     7021 gccttgtggc gtagccgcag ccgctcgatc tcgcgctcga ggccgcgtcg gtcgacgaac
     7081 tcgagcaagt agagtgcctc ttcgtcggcg atcgccgtca tcgggccgag cggtgtatcg
     7141 agccaggcgg cgaacaacgc gcgcgtcgac cgtgccggcg gcgcgcccat gatgcgtgcg
     7201 aacgcgtcgc gaaagccgct cgaagactcg aagccggcat cgagctgcgc gtcgatgacg
     7261 cgctcgcctg cacggatcga cttgaatgcg gagccgagcc gacgcgcgcg cgcgtactcg
     7321 acgaacgtca tgccgaagcg cttttggaac tggcgccgcg ccgtcgacgc gtgcacggcg
     7381 agcgcgtcga agtcggcgtc gcgccagcgt ttcgtcgggt cgctttcaac ggccgcgacg
     7441 agcttgcgca cgacgtcgga cgtctcgttc gggtgcgata gcggccggca ccgcgcgcag
     7501 ggccggtacg cggccagcag cgccgactgc gcgtccgtga agaactcgca gttctcgcgc
     7561 ttcggcttgc gggccgggca ggttggacgg cagaagattc cggtggtccg cacgccgacg
     7621 tagaagacgc cttcgtaatc ggcgtcgcgg cctagtaggg cggtgtagca ccggtcctga
     7681 tcgagcggca gttgtgtcgt atccatgggc ggcattctac ggccggcccg gcgccccgcc
     7741 gccgaaattc gcgcggctaa ttccggcccg gttcctgcgg cgtcttgctg ggtcgccgtc
     7801 gcggcttctg cgcctttggc ttgggcgtag cggccgcgcg aattgcgccg cgcgcccact
     7861 cggcgagctc cgtcggatct tcgagcacct cgatcggcac gcggaagtac gacatcgaga
     7921 cggggcggtc cgcgacagga cgaaacggct cgctgccagc ggcttcatac gcggcgcggt
     7981 tgcggtcgtc gactttgaag aacagctcgt ccgcggcgag gatgccgaag aagcgctcgc
     8041 cggaatagag accgacgccg ccgaacatcg ctttcggccg cagttgcggc acgcgcgcga
     8101 gctgatcgag cacgaactcg cgaaacgccg gcgaaacacg cagcgagcgc cgtggccggc
     8161 acttggtgcg cggccgcgcc atcctcaggc gcgcgggacg cgcacgccgc cgagcacggt
     8221 ttcgccgggg aacagccgct gccggccttt gagcttgttg tcgtagttgt cgacgaactc
     8281 gaattcgcct tcgcgcagct cgagcacggc gacatcggcc ggggcgccga cgttcagcgt
     8341 gccgcgatcg cggaacacgg cgaacgtccg cgcggcattg aaggttgcac aggcgacgac
     8401 gtcggctacc gacatgccga agccgagcag cttcgacatg cagttcggaa agtcgacgac
     8461 gcccgtcgtg ccgctcatga cgttccagtc cgtcgagatc gtgtcgggcc aaaagccgac
     8521 gcccatgatt tgctcgatgg tgtcccagcg catgtggtcc ctgacgccgt tgccgacgtc
     8581 gaacacgatg ccgcggcgcc gcgccgcgag cacctcgggg agcagcttgc cgttgttgtc
     8641 gacgatcgcg ttcggcggcg gcgcgaacag gtgcgtgacg atgtcgccgg tcttcagtat
     8701 cggcagcagc tcgcgcagcg gcgatgggct ctggcccatg tggatcatga cgggcaggcc
     8761 gagcggctcg acgacctgct gcgcgcgctt cagcacctcg acgtcgttct tgccgacgac
     8821 ggtctcggac aaacgcgctt tgatgccgat gacgtaatcg cggtgcttct cgatggcggc
     8881 gcgcgccgcg gcggcgtcgg cgagcttcaa atccttggtg tcgccgtcgg gcaggatgcc
     8941 gcctcgcccg atgttgacga gcactcggcc cggctgttgc gacgctcgcg cgacggccac
     9001 gacggcgtcg atgccatcgg cgccggccga gcccgcgtcg atccatcccg tcacgccgtc
     9061 gcgcagcagc atcgacgggc ccgccatgtc gcggcccgcg tgcgtgtgga tatcgatgag
     9121 gcccggcacg acgagcttgc cgcgggcgtc gagcgtcgcg gcggcttcgg ccgcgagcgt
     9181 cggctcgacg gcggcgatcc ggccccccgc gatgccgacg tcgcgcgtcg catcgagccg
     9241 caaggacgga tcgatgacgc gcccgccttt gatgatgagg tcgtagcgcc tgggctgggc
     9301 cgcaacccga tcggtggaga agagcgccgc gctcgcggcg gcgaaaatga gctcgcgcct
     9361 gttcatggcc gtcccccctt gtcggctatg cgccgagctt aagccagccc cgagcgctac
     9421 gtcacgattt gtcaaagcga gaagcacgcc gcagtcccac tgctaggcgc cggttaacgt
     9481 ggcggccatc cattcgacca acggcggtag ttgcgatgag aaccttagct ttggcgctag
     9541 tggcgacggc ggttgcgggc tcgagctatg cggcggacga gcccgtgcag ccgacgagct
     9601 cgtacttcga gggcgccaag atcacggtca atgcgcgcgc caaagccgac ggcttcatgc
     9661 gcgtacgggt cgtccccgag aacggcgagc cgcgcgaggc gacgttcggc atcgcgaaac
     9721 gcatgaacga gaacgacctc gccaaaggca tcgccgtgtc gctgaacacc gtgctgctcc
     9781 cggagtacgt cgccgacaag gacaacggcg agcacgtcaa gatccagaag aagcaaaaga
     9841 acgcgccgaa cttctcggtc gagatcacgt tcaacgcgcc gggcttctcc gtcatcctcg
     9901 acgactaaga cgcgcgcgac gcgcgcaagc gcggcgagtt gctcagccgg cgaccgcgag
     9961 cggcggcact ttcggccgcg tcgagctcca atcgcgcacg cgctgcaggg ccagctcgat
    10021 cagatgagct tcgccttgtg ggtccggcat gatgcacggc gggtggcggc gcagccgctc
    10081 ccaatcgccg ttctggatga cggtattcaa gaacgtcaga ttctttcgga tgaagcggat
    10141 gtacgggctc tggccgcgct cgaagaacgc ttcgaaatac cggtagacgc gattgaagtt
    10201 gttctcgagc cgcaaccgcg ccgaggtctg atagaacgat agcgtcttgc cgagcaggtc
    10261 ttgcgccgag tggtacagga ccttgccttg cggccgctcg tcgatcgcga caccgccgag
    10321 cacgaactcg gggtaaagcc ggtcgcggca cttctcgaga tagcaacggt cggcgagctg
    10381 cgcgaccaaa tccgccgtgc cgagcagatg gccgacgacg ctgtccttcg gctcttcgag
    10441 ctcgatctgg tcgacgttga gctcgtagcc cgtgaaatgc acgatgcgcg agacgactgg
    10501 cgcgaactgc tcgaggccga tgcgcggcaa gtagctctcg agatactggc cgctgcgcgt
    10561 cacgtgcacg cgcgtgaaca cggcgccgtt gacggccgcg gcgtcacgat cgcggtggcg
    10621 caggtagccg acgtcgtgga atagcgcgct gacgatcgcg agctcggcgc ggtcggggcc
    10681 gagtctgtcg ccgggctcga cgctgagctc gtggcccgcg atgagccgcg cgagcgcgag
    10741 agtcatgtcg agcgtgtgct gcatgtcgtg atacgtcgta tcgacggcgt gatagctcgg
    10801 gtcgagcccg cggaacacgc gctcgaaatc gtgaaaggcg agccagacgg aatcgaacga
    10861 gctgttcgga tagagcgcgc caaaaagatc cgcgacggcg ctacgcacct gcggggatga
    10921 gctcacctgc acggtgttgg taacgtcgta gtcgttacgt cggtcttgca gttcgtcttc
    10981 gatcggcatc ggagggtccg ttgaagccga caagtctaag agattcgacg cgttgctcgt
    11041 atcgtgcatg tcacataagc gctagctcgg cgtgaattct gcgactactg gcacgtggtc
    11101 ggaagggcgt tcccaaccgc gcggttcctt gtcgattcga cacgccgagc acgtttgact
    11161 taatgcacgc gaggccagca tgaggtcgat tctgagccct tggttacggc ggaaggcgcc
    11221 cgcgcggtag tcccaccagc tgaagacctt ctcgggctgc gaaaacagcc gaaacacgtc
    11281 gctcaagccc aaactacaga tcgccgccag cgccgcgcgc tcgcccggcg agcacatgat
    11341 tttgccttgc caggcggcgg gattgtgcac gtcgcgatcg tcgggcgcga tgttgaagtc
    11401 gccgacgagc aggagcgacg cgtgccgtgc gagctcggcc tcgagcgaga cgcgcagggc
    11461 ggcaagccac tcgagcttgt agccgtactt gtcggagccg acgctctggc cgttcgggac
    11521 gtataggctc aggagccgca acgagccgaa ggtcgcggcg agcacgcgct tttgctcgtc
    11581 ggcgtagtgg tcgccgatgc cgtaggcgac gtcgcttggc ggctcgcgcg acaggatggc
    11641 gacgccgttg taggcggctt gcccgctgaa cgtcgcgaca tagcccaagt cgcgaaactc
    11701 gtcggcgggg aagtcttcgt ccttgagctt ggtctcctgg agcgccagca cgtcgggacg
    11761 ctcgcgctcg agccacttca agacgtgctc cagcctcacc ctgaggctgt tgacgttcca
    11821 agtggcg
//
LOCUS       DATLEE010000747         5427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8916730, whole genome shotgun sequence.
ACCESSION   DATLEE010000747 DATLEE010000000
VERSION     DATLEE010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8916730"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1128
                     /locus_tag="VM692_16420"
     CDS             88..1128
                     /locus_tag="VM692_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HUQ53815.1"
                     /translation="MELYLDSVDFKEIEAALDYGFIKGLTTTPTFMHRHGITDIDGAI
                     VKLSNMVPELQVEALGDTPQQIVAEAERLVKLGLDTSKTVFKVPISNEGVKACSRLRA
                     KGYKVNVHLIYTLNQAYMAMEAGASFICPLAGRMQDQGHDAIQLFEQCVDVIDRYGYN
                     SKVMFSSVRHAEHVRQAMLTGVHVCTMPFSVMKNLCENTLTALGTAQFREHTALMTMR
                     VKDVVRKSNPVCDTNETLSSAMVKMTESRLGLVTVVDAKGNVAGVFTDGDLRRKLQSD
                     GRAILDKSLGAIGFSKTPHTLDRESLLNDAVKLFKKAEVDSIVVVDGGRPVGVLDVQD
                     LIKLGLLGQEHL"
     gene            1145..1837
                     /locus_tag="VM692_16425"
     CDS             1145..1837
                     /locus_tag="VM692_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HUQ53816.1"
                     /translation="MKPETELEVFSKLGGTFVTPIGTLVERARELRGFVSDWDGVFNQ
                     GTKGEGSFSTYSEPDSMGTNLLRYALWRRRSVLPIAALITGMENPSARNFALREHFHA
                     IYYGSRNKTAAIEALCRTHRCSSDQLLCMFDDVNDLGMAFACGIRVYVQRSASPLLRD
                     YVVRQGLCDYVTAHTAERHAVREVCELTLGLLGAFDAVVASRVGWDRDYATYFAARQA
                     QTTEFIDQRAQD"
     gene            1839..3170
                     /gene="waaA"
                     /locus_tag="VM692_16430"
     CDS             1839..3170
                     /gene="waaA"
                     /locus_tag="VM692_16430"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HUQ53817.1"
                     /translation="MSVWRVVVYLLLPYALGNLIWRALRYPAYWHRWSERFGFVAPLG
                     KLRTLWVHAVSVGEVRSAAPLVSELVERYPEHRVVVTTMTPTGSEQVKELFGDRVSHC
                     YVPYDFPDAVRRFFDRVRPEVAVIAETEFWPNIFAECGRRQIPLLLVNGRVSQASLRG
                     YLRVANTARRMLANADLLCAQTKVDAQRLRNLGAPPQLVHVTGNLKFDVELPAQLLDE
                     ARALRVQWGRERPVLIAASTHAGEERKVLDAFAELAPRFPRMLLVLVPRHPERFQAVA
                     RLCRKRGHSVALRSCTRGELPPGTDVLVGDTMGELQRLYAAADVAFIGGSLVPHGGQN
                     LLEACAVHVPVVFGPHMFHFEEISAMALERGAARQVHDVGDLSAAIALYFEQPDLRRA
                     AGNAAYTLVTDNRGALARTLELVEAALRVADLPPRSAGAKALEALAAPPRD"
     gene            3315..5243
                     /locus_tag="VM692_16435"
     CDS             3315..5243
                     /locus_tag="VM692_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ53818.1"
                     /translation="MKLIDFEIPVTASPESGAPPEGLVAFYWHFIRQTKGWYGAMFAA
                     SLLVALLDTVIPLFIGRLVSLMESPDRAAALAAALPMLLGMVALVVVVRPLAIFTDVS
                     IRHNALVPGATSLIRWQSHWHVVRQSWPFFQNDFAGRIANRVMQTASSLRESAMSSIR
                     AVWYIGVYGATAFALLTVADWRLGVPIFTWFVGYVLILKHFVPKLRELARVSSEGRSL
                     VMGRVVDSYTNILTVKLFGRMRDEDQYVREAMDGHYGAMRTHLRMITQFHTCLTVLNA
                     LLLVSTAAIGLTLWGRGVIDAGAVATALPLAWQTVTAAGWVSWEVTSIFENIGTVQEG
                     MQSIAVPHSSVDRAGARALEVPRGEIRFESLTFSYGRGDAAPVVHNLNLTIRPGERVG
                     LVGRSGAGKSTLVNLLLRLHDTESGRITIDGQDIAHVTQESLRSRVGMVTQDTSLLHR
                     SIAANIRYGKPSASDNEVAAAARKAQAHEFIGTLCDWHGRSGYEAHVGERGVKLSGGQ
                     RQRIALARVILKDAPILVLDEATSALDSEVELAIQEQLLDLMQGKTVIAIAHRLSTIA
                     RMDRLVVLEAGKVVEEGTHAELLRRGGHYEKLWRHQSGGFIANELDDAAAETAVDYQP
                     SAASAHLQSGPSRLPAVG"
     gene            5269..>5427
                     /locus_tag="VM692_16440"
     CDS             5269..>5427
                     /locus_tag="VM692_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ53819.1"
                     /translation="MNIVACDYETHAAPILAILNDAIVTSTALFDYRPREPASMLDWF
                     RAKDARRFP"
BASE COUNT          893 a         1793 c         1805 g          936 t
ORIGIN      
        1 gctctcgacg atctcgagag cttcatgcgg ccgctcatcg agatccacga cttggtacgc
       61 tcgcagtcgg tgcactaaag gactcgcatg gaactctatc tcgactccgt agacttcaag
      121 gaaatcgaag ccgcgctcga ctacggcttc atcaaggggc tcacgacgac gccgacgttc
      181 atgcaccggc acggcatcac ggacatcgac ggcgccatcg tcaagctgtc gaacatggtt
      241 ccggagctgc aggtcgaggc gctcggcgac acgccgcagc aaatcgtcgc cgaagcggag
      301 cggctcgtta agctcgggct cgacacgtcg aagaccgtgt tcaaggtgcc gatcagcaac
      361 gagggcgtga aggcgtgcag ccgcctgcgc gccaagggtt acaaggtcaa cgtgcacctc
      421 atctatacgc tgaaccaggc gtacatggcg atggaggccg gcgcttcgtt catctgcccg
      481 ctagccggcc gcatgcagga ccaggggcac gacgcgattc agctgttcga gcaatgtgtc
      541 gacgtcatcg accgttacgg ctacaacagc aaggtcatgt tctcctcggt gcgccacgcc
      601 gagcacgtac gccaggcgat gctgacgggc gtgcacgtct gcacgatgcc gttctcggtc
      661 atgaagaacc tctgcgagaa cacgctaacg gctctaggca cggcccaatt ccgcgagcac
      721 acggctttga tgacgatgcg cgttaaagac gtcgtccgta aatccaatcc cgtctgcgac
      781 acgaacgaaa cgttgtcgtc ggcgatggtc aagatgacgg agtcgcgcct cgggctcgtg
      841 acggtcgtcg acgcgaaggg caacgtcgcc ggcgtgttca cggacggcga cttgcgccgc
      901 aagctacagt ccgacggccg cgcgatactc gacaagtcgc tcggcgcgat cggcttctcg
      961 aagacgccgc acacgctcga ccgcgagagc ttgctgaacg acgccgtgaa gcttttcaaa
     1021 aaggccgagg tcgacagcat cgtcgtcgtc gacggcgggc gccccgtcgg cgtcctcgac
     1081 gtgcaagacc tcatcaagct cgggctgctc ggccaagagc atctctgacg cgcgaatcgc
     1141 agcgatgaaa cccgaaaccg agcttgaagt cttctccaag ctcggcggta cgttcgtgac
     1201 tccgatcggc acgctcgtcg agcgggcgcg cgagctccgc gggttcgtct ccgactggga
     1261 cggcgtgttc aaccagggca cgaagggcga gggctcgttc agcacctaca gcgagcccga
     1321 ctccatgggc acgaacctgc tgcgctatgc gctctggcgc cggcgcagcg tgttgccgat
     1381 cgccgctctg atcacgggca tggaaaaccc gtccgcgcgc aatttcgcgc tgcgcgagca
     1441 cttccacgcg atctactacg gctcgcgcaa caagacggcc gctatcgagg cgctgtgccg
     1501 cacccaccgc tgctcctcgg atcagctgct ctgcatgttc gacgacgtca acgatctcgg
     1561 catggcgttc gcgtgcggta ttcgcgtcta cgtgcagcgc agcgcgagcc cgctgctgcg
     1621 cgactacgtt gtccgtcagg ggctttgcga ttacgtgacg gcgcacacgg cagagcgcca
     1681 tgccgtgcgc gaggtctgcg agctcacgct cggcttgctc ggcgcgttcg atgcggtggt
     1741 cgcctcgcgc gtcggatggg atcgcgacta cgccacgtac ttcgccgctc gccaagcgca
     1801 aaccaccgag ttcatcgacc aacgcgcgca ggattgacgt gagcgtctgg cgcgtcgtcg
     1861 tctatctgct gttgccgtac gcgctcggca atttgatttg gcgcgcgctg cgctatcccg
     1921 cgtactggca tcgctggtcc gagcgcttcg ggttcgtggc gcccctgggc aagttgcgca
     1981 cgctgtgggt gcacgccgtg tccgtcggcg aagtgcgctc ggcggctccg ctcgtcagtg
     2041 agctcgtcga gcgctatccg gagcatcgcg tcgtcgtcac gacgatgacg ccgacgggct
     2101 ccgagcaggt caaggagctg ttcggcgacc gtgtgagcca ctgctacgtg ccgtacgatt
     2161 tcccggacgc cgtgcgccgc ttcttcgatc gcgtgcggcc cgaggtcgcc gtgatcgccg
     2221 aaacggagtt ctggccgaac atctttgccg agtgcgggcg ccggcagatc ccgctgctgc
     2281 tcgtgaacgg ccgagtttcg caagcttcgc tgcgcggtta cctgcgagtg gcgaacactg
     2341 cgcggcggat gcttgcgaat gccgacctgc tgtgcgcaca gacgaaagtc gatgcgcagc
     2401 gcttgcgcaa tctaggcgcg ccgccgcagc tcgtccacgt gaccggcaac cttaaatttg
     2461 acgtcgagct accggcgcag ttgctcgacg aggcgcgggc cttgcgcgtg cagtggggcc
     2521 gagaacggcc cgtcttgatt gcggccagca cccatgccgg cgaggaacgc aaggtgctcg
     2581 acgcgttcgc cgagctggcg ccgcgctttc cgcgcatgtt gctcgtgctc gtgccgcgtc
     2641 atcccgaacg atttcaagcc gtcgctcggc tatgtcgcaa gcgcggacat tccgtcgcgc
     2701 tgcgcagctg cacgcgaggc gagctgccgc ccggcacgga cgtgctcgtc ggcgacacga
     2761 tgggcgagct gcagcggctc tatgcggccg cggacgtcgc gttcatcggc ggcagcctcg
     2821 tgccgcacgg cggccagaac ttgctagaag cgtgtgccgt gcacgtgccc gtcgtgttcg
     2881 gcccgcacat gttccacttc gaagagatca gcgcaatggc gctcgagcgc ggcgctgcgc
     2941 gccaagtcca cgacgtcggg gacttgagcg cggccatcgc gctgtacttc gagcaaccgg
     3001 acttgcggcg cgccgcgggc aacgccgctt acacgctcgt gacggacaac cgcggcgctc
     3061 tggcgcgcac tctcgagctc gtcgaggccg cgctgcgcgt cgccgatttg ccgccgcgct
     3121 ccgccggcgc caaggctctc gaagcgctgg ccgcgccgcc gcgcgactga cgtcgccgtc
     3181 cggcgacggc ttgacaggtt ttcgggaacg cacggcgcgc acgcagagcg aatctgttat
     3241 cgtgcagcgc gccggccgat cgccggcgtt cccttcaatc gaggtacgcg gcgcacgctg
     3301 ctctgcacgc ttccgtgaaa ctcatcgact tcgagatccc agtgaccgcg agcccggagt
     3361 cgggagctcc acccgaaggg ttggtcgcgt tctattggca cttcatccgc cagacgaagg
     3421 gctggtatgg agcgatgttc gccgcgagct tgctcgtagc tctgctcgac accgtcatac
     3481 cgctgttcat cgggcggctg gtctcgctga tggagtcgcc ggatcgcgcc gccgcgctgg
     3541 cagccgcgct gccgatgctg ctcggtatgg tggcgctcgt ggtcgtcgtg cggccgctcg
     3601 cgatcttcac ggacgtctcg atccggcaca acgcgttggt accaggggcg acgagcctga
     3661 ttcgttggca gagccattgg cacgtcgtgc gccagagctg gccgttcttc cagaacgact
     3721 tcgccgggcg catcgcgaat cgcgtcatgc agacggcgag ctcgctgcgc gaaagcgcga
     3781 tgtcgagcat tcgtgcggtc tggtacatcg gcgtctatgg cgcgacggcg ttcgcgctgc
     3841 tgacggtcgc cgactggcgg ctcggtgtgc cgatcttcac gtggttcgtc ggctacgtgc
     3901 tgatcttgaa gcacttcgtg ccgaagctgc gcgagctcgc gcgcgtgagc tccgaaggcc
     3961 gttcgctcgt catgggccga gtcgtcgaca gctacacgaa catcttgacg gtcaagcttt
     4021 tcggccgcat gcgcgacgag gaccaatacg tacgcgaggc catggacggc cactacggcg
     4081 ccatgcggac gcacttgcgg atgatcacgc agttccacac gtgcctgacc gtgctgaacg
     4141 ccttgttgct cgtcagcacc gcggccatcg ggctcacgct ctgggggcgc ggcgtcatcg
     4201 atgcaggcgc cgtcgcgact gcgctgccgc tggcgtggca gaccgtgacg gccgcgggct
     4261 gggtcagctg ggaggtcacg tcgatcttcg agaacatcgg cacggtgcag gaaggcatgc
     4321 agtcgatcgc cgtgccgcac tcgagcgtcg accgcgcggg cgcgcgagcg ctcgaggtgc
     4381 cgcgcggaga gatccgcttc gaaagcttga cgttctcgta cggccgcggc gacgcagcgc
     4441 ccgtcgtgca caacttgaac ctcacgatcc gccccggcga gcgcgtcggg ctcgtcggcc
     4501 gctcgggggc cggcaaatcg acgctcgtga acctgctgct gcggctgcac gacaccgagt
     4561 cgggccgcat cacgatcgac ggccaagaca tcgcgcacgt cacgcaggag agcctgcgct
     4621 cacgcgtagg catggtcacg caggatacgt cgttgttgca tcggtcgatc gcggccaaca
     4681 tccgctacgg caagccgagt gcaagcgaca acgaggtcgc cgccgcggcc cgcaaggcac
     4741 aagcgcacga gttcatcggg accttgtgcg attggcacgg gcggagcggc tacgaggcgc
     4801 acgtcggcga gcgcggcgtg aaactgtcgg gcggccagcg gcagcgtatc gcgctcgctc
     4861 gcgtgatctt gaaggacgcg ccgatcttgg tgctcgacga ggcgacgtcg gcgctcgaca
     4921 gcgaggtcga gctcgcgatc caggagcaac tgctcgatct gatgcagggc aagaccgtga
     4981 tcgcgatcgc gcaccggctg tcgacgatcg cgcgcatgga ccggctcgtt gtgctcgagg
     5041 ccggcaaggt cgtcgaagag ggcacgcacg ccgagctgct gcgtcgcggc ggccactacg
     5101 agaagctctg gcgccatcaa tccggcggct tcatcgcgaa cgagctcgac gacgcggcgg
     5161 ccgaaacagc cgtggactac caaccgtcgg ctgcctccgc gcacttgcag tcggggccct
     5221 cgcggctgcc ggcggttggc tgaagcgcgc gagccgaggt cgcaaacgat gaacatcgtt
     5281 gcctgcgact acgagacgca cgccgcgccg atcctcgcga tcttgaacga cgcgatcgtg
     5341 acgtcgacgg cgctgttcga ttatcggccg cgcgagccgg cgagcatgct cgactggttt
     5401 cgcgccaaag acgcgcgccg ctttccc
//
LOCUS       DATLEE010000748         2574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7425800, whole genome shotgun sequence.
ACCESSION   DATLEE010000748 DATLEE010000000
VERSION     DATLEE010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7425800"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VM692_16445"
     CDS             complement(<1..524)
                     /locus_tag="VM692_16445"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HUQ53820.1"
                     /translation="MDSLQIVVLAVVQGITEFLPISSSGHLILVPYFTSWPDQGLDFD
                     LAVHIGTLTAIVTYFRRTLRVMARDWARSVAERREVGESRLAWAVLFGTIPAGIVGLL
                     FRHDIETTLRSPLVVATTTIGYAVLLLIADRVGAKQRDERSIGWLDVVVIGCAQALAL
                     VPGTSRSGVTITAG"
     gene            778..978
                     /gene="tatA"
                     /locus_tag="VM692_16450"
     CDS             778..978
                     /gene="tatA"
                     /locus_tag="VM692_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HUQ53821.1"
                     /translation="MSVPQLLIILVIVALIFGTKRLKEVGGDLGSAVKSFRKAMNTAN
                     EVSDESAEPPKQLISDEKEKTK"
     gene            1036..1995
                     /gene="secF"
                     /locus_tag="VM692_16455"
     CDS             1036..1995
                     /gene="secF"
                     /locus_tag="VM692_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HUQ53822.1"
                     /translation="MHWQWLTGSGTPNFNFVKYRRVAMMTSAILSFVAIVSFFYPGLN
                     YGIDFTGGTIVEAAYTNPPDLDAIRGDLTEAGFESVQAQNFGSANDVLIRLPPAEGED
                     ASDPAAVTALQNRVQDVLRAHDADVEIRSTATVGGQVGEDLFENAGLALLFALILVFA
                     YVMLRFRWKLAVGAIVATLHDVIVTSGIFALAGWQFDLTVLGSILAVLGYSLNDTIVV
                     YDRIRDNFRLMRRGSALEIVNASVNQTLARTLVTGVTSLLVLFALMLLGGDTLWGFSV
                     ALIIGIVVGTYSSIYIASSMALALDISPSDFTVTKQERVDELP"
     gene            2005..2541
                     /locus_tag="VM692_16460"
     CDS             2005..2541
                     /locus_tag="VM692_16460"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HUQ53823.1"
                     /translation="MSRRVQLERPTARRERDFLAAALRSRALHRGFVAPPASPSEYRD
                     YLRRSRRDEQSSYFVVEPESGALAGVINLNDIVRYSEQSARLGYYAFTPLARAGLMRA
                     GLVLVIGIAFRELGLHRLEANVQRSNVRSIALVEGLGFVREGTARGFLKIGNRWRDHE
                     RWALLKEDWRDAPDRLAD"
BASE COUNT          459 a          823 c          840 g          452 t
ORIGIN      
        1 aagccggcgg taatcgtgac gcccgagcgc gaggtgcccg gcacgagcgc gagcgcctga
       61 gcgcagccga tgacgacaac gtcgagccaa ccgatcgagc gctcgtcgcg ctgcttggcg
      121 ccgacgcggt ccgcgatcaa cagcaacacg gcatagccga tcgtcgtggt ggcgacgacg
      181 agcggcgagc gcagcgtcgt ctcgatgtcg tgccggaaca gaagcccgac gatgccggcg
      241 gggatggtgc cgaagaggac ggcccacgcg agccggcttt ccccgacttc gcgccgctcg
      301 gcgacggagc gcgcccagtc gcgcgccatg acgcgcaagg tgcgccggaa gtacgtgacg
      361 atggccgtca acgtgccgat gtgcacggcg aggtcgaagt cgagaccctg gtcaggccag
      421 ctcgtgaaat aggggaccaa gatcagatgg cccgagctcg atatcggcaa gaattcggtg
      481 atcccctgga cgacggcgag gacgacgatc tgcaaagaat ccatgagacc cgaccgggag
      541 ctcccttaac cgcgcgtaat gttagccgcg aatggcggcc ccgcaacgca accaggccac
      601 tttcccggcg cgaaaggcgt gaactgtcgc gcagctaccg cggtcaatag ggggcaagct
      661 cggcctttga gctttagacg gcgtcgggtt ggcttttcgg cctcggaccc tcaaaatgat
      721 gtctcggcgg cgccaaatcg gcagctcgtc gaatggagag agcgtatggg tctcgcgatg
      781 agcgttccgc agctgctgat cattctcgtg atcgtggctt tgatcttcgg caccaagcgg
      841 ctcaaagagg tcggtggcga cctcggcagc gccgtgaaga gctttcgcaa ggccatgaac
      901 accgcgaacg aggtctcgga cgagagcgcc gaaccgccga agcagctgat aagcgacgaa
      961 aaagaaaaga ccaagtaaac tgcgcccgtt tttcctaccg cgctaacgcg ctcgctgccg
     1021 tgcggcagct cgactatgca ctggcagtgg ctaacaggct ccggcacccc taacttcaat
     1081 ttcgtgaagt accgccgggt ggcgatgatg acatcggcca tcctgtcgtt cgtcgcgatc
     1141 gtctccttct tctatccagg cctcaattac ggcatcgact tcacaggcgg caccatcgtc
     1201 gaggccgcct acacgaatcc gcccgacctc gatgcgattc gcggggatct gaccgaggcg
     1261 ggcttcgagt ccgtgcaagc gcagaacttc ggctcggcga acgacgtgct gatccgcttg
     1321 ccgccggccg aaggagagga tgcgagcgat ccggcagcgg tcacggcatt gcagaaccgc
     1381 gtgcaggacg tgctgcgcgc acacgacgcg gacgtcgaga ttcgcagcac ggcgaccgtc
     1441 ggcggccaag tcggcgagga cctgttcgag aacgcgggcc tcgcgctctt gttcgctctg
     1501 atactcgtgt tcgcctacgt catgctgcgc ttccgctgga agctcgccgt cggcgccatc
     1561 gtcgccacct tgcacgacgt catcgtgacg agcggcatct tcgcgctcgc gggctggcag
     1621 ttcgacctca cggtcttggg ctcgatcctc gcggtgctcg gctactcgct gaacgacacg
     1681 atcgtcgtct acgaccgaat tcgcgacaac ttccgcctca tgcgccgcgg gtccgcgctc
     1741 gagatcgtca acgcctcggt caatcaaacg ttggcccgaa cgttggtgac cggcgtgacg
     1801 agcttgctcg tgctgttcgc gctgatgctg ctcggcggcg acacgctctg gggcttttcc
     1861 gtcgcgttga tcatcggcat cgtcgtcggc acttattcgt cgatctacat cgcgagctcg
     1921 atggcgctgg cgttggacat atcgccgtcc gacttcacgg tcacgaagca agaaagggtc
     1981 gacgagctgc cctagactcg gctcgtgagc aggcgagttc agctcgagcg acccacggca
     2041 cggcgcgaaa gggatttcct tgctgcggcg ctgcgcagtc gggctctgca ccgcggcttc
     2101 gtcgctcccc ctgcgagtcc cagcgagtat cgcgactatt tgcgccgttc gcggcgcgac
     2161 gagcaaagct cgtacttcgt cgtcgaaccc gagtcgggcg cgctggccgg cgtcatcaac
     2221 ttgaacgaca tcgtgcgcta ttccgagcaa tcggcgcggc tgggctacta cgcgttcacg
     2281 ccgttggcgc gcgcgggtct gatgcgcgca ggactcgtgc tcgtgatcgg catcgcgttt
     2341 cgcgagctcg gcctgcaccg cctcgaagcc aacgttcagc gcagcaacgt gcgctcgatc
     2401 gcccttgtcg agggcctagg cttcgtgcgc gaaggcacgg cgcgcggttt tttgaagatc
     2461 ggcaaccggt ggcgcgatca cgagcggtgg gcgctgctga aagaagactg gcgcgacgct
     2521 cccgaccgct tggccgatta ggccggcgcg cgggcgtagc cgagcggcag tgcc
//
LOCUS       DATLEE010000749         3736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11146270, whole genome shotgun sequence.
ACCESSION   DATLEE010000749 DATLEE010000000
VERSION     DATLEE010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11146270"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VM692_16465"
     CDS             <1..1117
                     /locus_tag="VM692_16465"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUQ53824.1"
                     /translation="ESLTGETAAVVVNVFGEDLDVLDRSGARIAASLGAVPGVVDLQY
                     ERQSSTPEILVRPRPEALAAYGVTRRDLLDVLQTAFAGLKVGQAFTGARAVDVVVILP
                     ATERNRAETIGRLIVQSPFGPVPVAQLAAVAPAVGRSQVRHLDGQRRVAVTFNVEGRD
                     LQGTVAAARERIAALGPLPAGVLVRFAGEAEAERQARFEIVLYSAFALVAVMLILFVC
                     FRRRAYPWLVVANLPFSLLGSIAVVGVTGIGLSLGTLVGLVTVFGVSSRNAILLLAHY
                     EHLLDVDGLPWNRATALRGASERLVPILMTALVTALGLTPLAMGVGRAGYEIEAPMAI
                     TVLGGLVTSTLLNLLVLLALAERAGSVTSNQSVLPLP"
     gene            1285..2949
                     /locus_tag="VM692_16470"
     CDS             1285..2949
                     /locus_tag="VM692_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53825.1"
                     /translation="MHHTIRPALASVCLALWLLPEQPVVSQETPPSTPDPATVRADLE
                     RAAATIRSVNVSVDDVFDPNDPKENKRLYRLANRLHISTREQVIESALLFDSGDVFEG
                     RLLDESARALRALGFVAAATVEPHDYDAATNSVEIDVRVRDSWTLATDMKFHHSGGVS
                     EFGAGLEDRNLLGMGKELKVSYRSLIERDETYVGYIDPHLFGSRVQLSTVLANASDGH
                     RRELAVDRPFYSLDTRWSLGGSLRDEQRVDPIYDLGEEIDEFSHDIRSSTLQGGWSRG
                     LIDGKTRRWLMGVTAEEHRFAPTLDTPQPLLLPVDRELVYPWVGWQLVEDDFREMSEL
                     NDLGRTEDISLGLNLFFQVGFAKQRFGSDRDATVYKASAQTGWEPGGPGRLLLLDAGG
                     STRDEDTGLQNSQMYVDLKFYQRNLTRHLFSVSLSALATNNLDAENQVLLGGDSGLRG
                     YPVRYQAGKHRSIVTVEERFFTDWYPGRLFRVGYALFADAGQVRGRDPRASPSQGTLY
                     DVGVGLRLSSPRATGRSIVHVDLAFPLNGDPSIDSVQLIVETKGSF"
     gene            2965..>3736
                     /locus_tag="VM692_16475"
     CDS             2965..>3736
                     /locus_tag="VM692_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53826.1"
                     /translation="MTYRIVVAALSAAHAAAALAAENIHFLAEHAVESGMDAVYTALP
                     WPSGRLAPDHWQPSVDLAAAHTTTEFMRLEGNSVAAAANRGVNPRWGYELMASYGASD
                     LSGGNGRVPLSVGFLDHVPLDLPQLADFSAVSGSQRQLSLGAAAVHERRTAASPYSSQ
                     LIAGLLLERVEVTGFRMQYLLLAGADAATSGTLEFSSRAGFVTPFVAWQQTRALAPRW
                     TWSLRAMLAAPLPPAELDARLAAPRLGVSVASMRSAIEL"
BASE COUNT          609 a         1224 c         1202 g          701 t
ORIGIN      
        1 cgagagcttg acgggcgaga ccgcggcggt cgtcgttaac gtgttcggtg aagacttgga
       61 tgtgctcgat cgcagcggcg cgcggatcgc ggcgagcctc ggcgccgtgc cgggcgtcgt
      121 cgacctgcaa tacgagcgcc aaagctcgac gcccgagatt ctcgtgcggc cgcgccccga
      181 ggcgctcgcc gcgtacggcg tgacgcggcg cgacttgctc gacgtgctgc aaacggcgtt
      241 cgctgggctc aaagtgggac aggcgttcac gggggcccgt gcggtcgacg tcgtcgtgat
      301 tctgccagca accgaacgca atcgcgccga gacgataggt cggctcatcg tacagagccc
      361 attcggcccc gtgccggtcg cgcagctcgc cgccgtagcg cctgctgtcg gtcgctcgca
      421 agtacggcac ctcgacgggc aacgtcgcgt cgccgtgacg ttcaacgtcg aaggtcgcga
      481 tctgcaggga acggtcgcgg ctgcccgcga gcgcattgcc gcgctcgggc cgttgcccgc
      541 aggtgtgctc gtgcgctttg caggcgaggc agaagcggag cgccaagcac gtttcgagat
      601 cgtgctgtat tccgcgttcg ctttggtcgc cgtgatgctg atcttattcg tgtgctttcg
      661 ccgacgcgct tatccctggc tcgtcgttgc gaacttgccg ttcagcttgc ttggtagcat
      721 cgcggtggtc ggcgtaaccg gtatcggctt gtcactcggc acgctcgtcg ggctcgtgac
      781 cgtgttcggc gtgagctcgc gcaatgcgat cctgctgctc gcccactacg agcatctgct
      841 ggacgtcgac ggacttcctt ggaatcgcgc gaccgcgctg cgaggcgcat cggaacggct
      901 cgtgccgatt ctgatgacgg cactcgtcac ggcgctcgga ctcacgccgc tggcaatggg
      961 cgtcggacgc gccggctacg agatcgaagc accgatggcc atcacggtgc tcggcgggct
     1021 cgtgacctcg acgctactca atctgctcgt cttgctggcg ctcgcggagc gcgcgggcag
     1081 cgtcacttcg aatcagtccg tgctcccctt gccatgatcc gcgagctctc gctacacgcg
     1141 ccgctcggcc gagcatacga gttcgcgcgc cgcaaccttg aatagacggt cgtccgggaa
     1201 aaaatcggtc gcggaccaca gagactgatt ttgcgcggtc tgctcgtcta acggagtacc
     1261 gtggcttggt cccagatatc tttcatgcat cacacaatac ggccggcgct cgcgagcgtg
     1321 tgcctcgctt tgtggctttt gccggagcaa ccggtagtaa gtcaggagac gccgccgagc
     1381 acgccggatc ccgcgacggt tcgtgccgat ctcgaacgcg ctgccgcgac gattcgtagc
     1441 gtcaacgtct cggtcgacga cgttttcgat ccgaacgatc cgaaagagaa caagcggctc
     1501 taccgcctgg cgaatcgcct gcacatcagc acgcgcgagc aggtcatcga gagcgcctta
     1561 ctgttcgatt cgggcgacgt tttcgaagga cgtctgctcg acgaatctgc gcgcgcgtta
     1621 cgcgcgctcg gcttcgttgc agctgccacg gtcgagccgc acgactatga cgctgcgacg
     1681 aatagcgtcg agatcgatgt ccgcgtacgt gattcatgga cgctggcgac ggacatgaaa
     1741 tttcatcaca gcggcggcgt cagcgagttc ggcgcgggtc tcgaagatcg caacctgctc
     1801 ggcatgggta aggagctcaa agtctcgtac cgcagcctca tcgagcgcga cgagacttat
     1861 gtcgggtaca tcgatccgca cctcttcggc agccgcgtac aactgagcac ggtgcttgcg
     1921 aacgcgagcg atggccatag gcgcgagctc gcagtggacc gcccgttcta ctcgctcgac
     1981 acgcgttggt ccctcggcgg ctcgctgcgg gacgagcaac gagtcgaccc aatctacgat
     2041 ctcggcgaag agatcgacga attcagccac gacatacgct cgtccacgct gcagggcggc
     2101 tggtcgcgcg gtttgataga cggtaaaaca cgtcgctggt tgatgggagt gaccgcggag
     2161 gagcaccggt ttgcaccgac gctcgatacg ccgcaaccgc tccttttacc cgtcgaccgt
     2221 gagctcgtgt acccctgggt cggttggcaa ctcgtcgagg acgacttccg cgagatgagc
     2281 gagctcaacg acctcggccg cacagaagac atttcgcttg ggctgaatct ctttttccag
     2341 gtcggcttcg ccaagcagcg ctttggctcg gaccgcgatg ccactgtcta caaagcttcc
     2401 gcgcagacgg ggtgggagcc cggcggcccg ggacggctgc tgctgctcga tgccggcggc
     2461 tcgactcgtg acgaggacac gggcttgcag aattcgcaga tgtacgtcga cctcaagttc
     2521 taccagcgta atctcacgag gcacttgttc tcagtgagct tgagcgcgct cgcgaccaac
     2581 aacttggacg cggagaacca agtgctgctc ggtggagaca gcgggttgcg cggctacccc
     2641 gtgcgctatc aggccgggaa acatcgctcg atcgtcacgg tcgaggagcg gttcttcacc
     2701 gattggtacc ccgggcggct gtttcgcgtc gggtacgcct tgttcgccga tgcgggccag
     2761 gtacgcggcc gcgatccgcg tgcttcaccg agccagggca ccttgtacga cgtcggcgtc
     2821 ggcctgcggt tgagctcgcc gcgcgccacc ggccgctcga tcgtgcacgt ggatctcgcg
     2881 ttccccttga acggcgatcc gagcatcgac agcgtccagc tcatcgtcga aaccaagggt
     2941 tcgttctagc gactcggcac gacggtgact taccgaatcg tcgtcgccgc gctgtcggct
     3001 gcgcacgcag cagcggcgtt ggccgccgag aacatccact ttctcgccga gcacgcagtc
     3061 gagagcggca tggatgcggt ctatacggcg ctaccgtggc cctccggacg gctcgcgccc
     3121 gatcattggc aaccgtcggt cgatcttgca gccgcgcaca cgacgacgga attcatgagg
     3181 ctcgagggca actcggttgc agcggccgcc aatcgcggcg tcaatccgcg ttggggctac
     3241 gagctcatgg cctcttacgg cgcctcagat ctgtccggcg gcaacggacg cgtgccgctt
     3301 tccgtcgggt tcctcgacca tgttccgcta gacctcccgc agctcgccga cttcagcgcc
     3361 gtcagcggct cgcaacgaca gctgagcctg ggggccgccg ccgtccacga gcggcgcaca
     3421 gccgcttcgc cctactcctc gcaactgatc gcgggactgc ttctcgagcg cgtcgaagtc
     3481 acgggctttc gaatgcagta cctgctcctc gcgggggccg acgcggcaac ctccggcacg
     3541 ctcgaattca gcagtcgcgc gggcttcgta acgccgttcg tggcatggca acagacgcgt
     3601 gcacttgcgc cccgctggac ctggtcgcta cgcgcgatgt tggccgcacc gttaccgccc
     3661 gccgagctcg acgctcgact cgcagcaccg cggctcggcg tgtccgtcgc ctccatgagg
     3721 tcggcgatcg agctcg
//
LOCUS       DATLEE010000750         5257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_3722868, whole genome shotgun sequence.
ACCESSION   DATLEE010000750 DATLEE010000000
VERSION     DATLEE010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3722868"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VM692_16480"
     CDS             <1..637
                     /locus_tag="VM692_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53827.1"
                     /translation="RAPSVDRAPRRGVLDSNATRTREGTATYRGSERAPGASGARTVE
                     RAPTTAGTANTTPQPRRGVPSSSYRNRSIESTDRASQAAQASRTPSGQPRAPGVDRQT
                     STPTRQAPPSNGGYRTGGGMSQARERMQSEASRSVPRATQPSTGMPREAPRSFESQSS
                     PPRAPSGGGMSQAQRGGGDGGGYRNGGGGGDGGGGREHPASRGSEGRSAGR"
     gene            complement(638..1492)
                     /locus_tag="VM692_16485"
     CDS             complement(638..1492)
                     /locus_tag="VM692_16485"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HUQ53828.1"
                     /translation="MDFVYFLGRFHVLALHLPIGIIVALCVLEVLSRKERYRYLEAAS
                     PYLWGATALTALVTVLLGFMHFAEGSFAGPSADQHRFFGTVVSIVATGVALLRASSFA
                     SSYKPLFMPASLVLLVLVSITGHYGGNLTHGSTFLVEYAPQPLRSLAGLAPRRTVTSV
                     SAADPFADVVGPMFVARCGGCHNEDKQESDLVLTTYAGIMRGGESGRVVVAGNTDLSE
                     LLRRISLPHDDDEFMPADGKTPLTEQQVEIIRWWIAAGAPNGGTVGALEVPAEMRDTL
                     SRELGVSF"
     gene            complement(1588..3141)
                     /locus_tag="VM692_16490"
     CDS             complement(1588..3141)
                     /locus_tag="VM692_16490"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HUQ53829.1"
                     /translation="MKKSHVPVLVVGAGAAGTMLTLELARRGVNVRTIDRLPKPGDTS
                     KAITVHARTLEMLERIDTRLVDRFLERGIHNLGYVLHFVDAAGKRSEVRPGIDFTTVD
                     SRYNFLLVHRQSESEQYLREYTSAHFGVAPDWNTACVDVQQDADGVTATLESNGVQEQ
                     VHCNYLVGCDGPNSRVRRAAGFEQDESDYKGTQLQNLDAFLNNFPDVDDYVHYCAGTD
                     HFIMIVKLPGGFYRMLLSDRGEAAGPNVTPEQGFMRLVNKHFDGVTLGDVVWHSKWQS
                     FVRLAHTYRKGRVFLAGDSAHIHSTTGGQGLNCCMQDSYNLGWKLAFVVKKLANASLL
                     DTYEPERRPIAEQVIWAASSLHEIFMGHGKDIAERAQKIRDPVFLEAVVGRCSGISYT
                     YRDQTQPPKASGAGPTVGDRAPDVELGRGRTLFDLTRHPSCTLIAVAAREAGQAALQA
                     SLKGIAHRFAAVLELRVIASSPAFVQRYGASDDDQLVLLRPDGYVGFRCKAADVAALE
                     SRLHEWFAL"
     gene            3212..3763
                     /locus_tag="VM692_16495"
     CDS             3212..3763
                     /locus_tag="VM692_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUQ53830.1"
                     /translation="MNLDTLTLETTRLLLRPTRRHDFEEFAKFQADAESMRYLGSAPQ
                     PRSIAWRSFLTMAGAWAMQGFAMFSVIEKTSGRWVGRVGPWQPEGWPGTEVGWGIASD
                     RCRLGYATEAATAAIDWAFAELGWSEVIHVIDPNNVASQGVARKLGSRNRGRAQLPAP
                     FAESVIDVWGQSKDEWLAARARH"
     gene            complement(3772..4173)
                     /locus_tag="VM692_16500"
     CDS             complement(3772..4173)
                     /locus_tag="VM692_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HUQ53831.1"
                     /translation="MHSRNWYSRFAKDAARFCGRPHVFAASIAIIVVWAISGALFHFS
                     DTWQLVINTATTIVTFLMVFLIQNTQNRDTEAIQIKLDELIRATKGAHNAILDLEDLD
                     DAAVDKFRRKYHVLAERARRGKPKEHDVPDE"
     gene            4325..>5257
                     /locus_tag="VM692_16505"
     CDS             4325..>5257
                     /locus_tag="VM692_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HUQ53832.1"
                     /translation="MRHAIVPAVVSGLLALSVARAQPSGAPAAESDARLAALKSAAIA
                     EVEQRRTLTQQIVDSLFSFSELGYQEFETQRYLTALLGRNGFTVERGVASIPSSWVAR
                     WGNGRPVIALGADVDGLPTTNQTPGVLTRRELVPGAPGHGEGHNSGPALVITAALAAK
                     AVMERERLPGTLVVWPGIAEELLAGKAYFVRAGVFADVDAVLYAHVNSVLATEWGDAD
                     TSSAVSVEYTFTGQSAHAARAPWLGRSALDAVELMNVGWNYRREHLRLAQRSHYVVSN
                     GGDQPNVVPPLASVWYYFRENDFAHVQDLWTIGNQ"
BASE COUNT          914 a         1787 c         1732 g          824 t
ORIGIN      
        1 ccgcgctccg tcggtcgatc gcgcgccgcg ccgcggcgtg ctcgattcga acgcgacgcg
       61 tacgcgcgag ggcacggcga cctatcgcgg ctcggaacgc gcgccgggag cgtcgggcgc
      121 tcgcaccgtc gagcgtgcac cgacgacggc cggcacggcg aacactacgc cgcaaccgcg
      181 tcgcggcgtg ccgagctcga gctatcgcaa ccggagcatc gagagcacgg atcgtgcgtc
      241 gcaagccgca caggcgagcc gcacaccgtc gggccagcca cgcgcgcccg gcgtcgaccg
      301 ccaaacgtcg acgccgacgc ggcaggcgcc gccgagcaac ggcggctatc gcaccggcgg
      361 cggcatgtcg caagcgcgcg agcgcatgca atccgaggcg agccgttccg taccgcgcgc
      421 gacgcaaccg tcgacgggca tgccgcgcga ggcaccacgc agcttcgagt cgcagtcgag
      481 cccgccgcgc gcaccgagcg gcggcggcat gagccaagcg caacgcggcg gcggtgacgg
      541 cggcggctac cgcaacggcg gtggtggcgg cgacggcggc ggcggccgcg agcacccagc
      601 gtcgcgcggc agcgaaggtc gctcggcggg tcgctagcta gaagctgacg ccgagctcgc
      661 gcgacagcgt gtcgcgcatt tcggcgggga cctcgagcgc gcctaccgtg ccgccgttgg
      721 gcgcgcctgc cgcaatccac caacggatga tctcgacctg ttgctcggtc agcggcgtct
      781 tgccgtcggc aggcatgaac tcatcgtcgt cgtgcggcaa cgagatgcgc cgcaacagct
      841 cgctcaagtc cgtgttgccc gcgacgacga cgcggccgct ctcgccgccg cgcatgatcc
      901 ccgcgtaggt cgtcaggacg agatcgctct cctgcttgtc ctcgttgtgg caaccgccgc
      961 aacgcgcgac gaacatcggg ccgacgacgt ccgcgaaggg atcggccgcc gacacgctcg
     1021 tgaccgttcg ccgcggcgcg agacccgcga gcgaacgcag cggctgcggc gcgtactcga
     1081 cgaggaacgt cgagccgtgc gtcaagttgc cgccgtaatg acccgtgatc gaaacgagca
     1141 cgagcaacac gagcgacgcc ggcatgaaca acggcttgta gctcgaagcg aagctgctcg
     1201 cgcgcagcag cgcaacaccc gtggcaacga tcgagacgac cgtgccgaag aaccgatgct
     1261 gatcggccga cggcccggca aagctgccct ctgcgaaatg catgaacccg agcagcacgg
     1321 tgacgagcgc cgtcagcgcc gtcgcgcccc aaagatacgg cgatgctgct tcgagataac
     1381 gataacgttc cttgcggctc aaaacttcga gcacgcagag cgcgacgatg atgccgatcg
     1441 gcaaatgcag agcgagaacg tggaaccggc ccaagaaata cacgaagtcc atgcagcaat
     1501 tcctatgcgc caaaatcggg acggcgcata cgataacagc tcgtcgtgcg caacgagaaa
     1561 cgtgcgggcg tggagcgcct taagacttca aagcgcgaac cactcgtgaa gacgagattc
     1621 gagagcagcg acgtcggcgg ctttgcagcg gaagccgacg taaccgtcgg ggcgcagcag
     1681 caccagctga tcgtcgtcgc tggcaccgta gcgctgcacg aacgcagggc tcgatgcgat
     1741 gacgcgcaac tcgagcaccg ccgcgaaccg atgcgcgata cctttgaggc tcgcctgtaa
     1801 agccgcttgc cctgcctcgc gcgccgcgac ggcaatcaac gtgcacgacg ggtggcgcgt
     1861 cagatcgaac aacgtgcggc cgcggccgag ctcgacgtcc ggcgcgcggt cgccgacggt
     1921 cgggcccgca cccgaggcct tcggcggctg cgtctgatcg cgatacgtgt acgagatacc
     1981 cgagcaccgg cccacgacgg cttcgaggaa cacgggatcg cggattttct gcgcgcgctc
     2041 ggcgatatcc ttgccgtggc ccatgaaaat ctcgtgcagc gacgacgcgg cccagatcac
     2101 ctgctccgcg atcggccgcc gctccggctc ataagtgtcg agcagcgacg cgttggcgag
     2161 cttcttgacg acgaacgcga gcttccagcc caagttgtag ctgtcttgca tgcagcaatt
     2221 cagcccctgc ccgcccgtcg tcgagtgaat gtgcgccgaa tcgccggcga gaaatacgcg
     2281 gcccttgcga tacgtgtgcg cgagccgcac gaagctctgc cacttcgagt gccaaacgac
     2341 gtcgccgagc gtcacgccgt cgaagtgctt gttcacgagc cgcatgaacc cctgctcggg
     2401 cgtcacgttc ggtcccgctg cttcgcctcg atcgctgagc agcatgcggt agaacccgcc
     2461 cggcagcttc acgatcatga tgaagtgatc ggtgcccgcg cagtagtgca cgtaatcgtc
     2521 gacgtcgggg aagttgttca agaatgcgtc gagattctgc agctgtgtgc ccttgtagtc
     2581 gctctcgtcc tgctcgaaac ccgcggcgcg ccgcacgcgc gaattcgggc cgtcgcagcc
     2641 gacgagataa ttgcagtgca cctgctcctg tacgccgttg ctctcgagcg ttgccgtcac
     2701 gccatcggca tcctgctgca cgtcgacgca agcagtgttc cagtccggcg cgacgccgaa
     2761 atgcgcgctc gtgtactcgc gcaagtactg ctcgctttcg ctctgccggt gcacgagcag
     2821 aaagttgtag cgcgaatcga cggtcgtgaa atcgatgccg ggacgcacct cgctgcgctt
     2881 gccggccgcg tcgacgaaat gcagcacgta gcccaggttg tgtatgccgc gctcgaggaa
     2941 tcgatcgacg agccgcgtgt cgatgcgctc gagcatctcg agcgtgcgcg cgtgcacggt
     3001 gatcgctttc gacgtgtcgc cgggcttcgg caagcggtcg atcgtccgca cgttgacgcc
     3061 gcggcgcgcg agctcgagcg tcagcatggt gccggccgca ccggcaccca cgacgaggac
     3121 tggaacgtgg ctcttcttca tcgcaagcct ccgccttcga gtatagccac ctgaatcgac
     3181 tcgcgccgac cgcgcgcgca agattgcccg catgaatcta gacactctca ctctcgaaac
     3241 cacgcgattg ctgctgcgcc cgacgcggcg gcatgacttc gaggaattcg ccaaattcca
     3301 ggccgacgcg gaatcgatgc gctacttggg ctcggcacct caaccgcggt cgatcgcctg
     3361 gcgcagcttt ctgacgatgg ccggcgcctg ggcaatgcaa gggttcgcca tgttctccgt
     3421 catcgagaaa acatccggcc gctgggttgg ccgcgtcggc ccgtggcaac ccgaaggctg
     3481 gcccggcacc gaggtcggct ggggcatcgc gagcgaccgc tgccggctcg gctatgcaac
     3541 cgaagccgcg accgccgcga tcgattgggc gttcgcggag ctcggctgga gcgaagtcat
     3601 ccacgtcatc gacccgaaca acgtcgcctc gcaaggcgtc gcgcgcaagc tcggctcgcg
     3661 taaccgcggt cgcgcccagc ttcctgcgcc attcgccgag agcgtcatcg acgtctgggg
     3721 ccaatcgaaa gacgagtggc tggccgcccg cgcgcggcac tgatcgctgg ctcactcgtc
     3781 ggggacgtcg tgctccttcg gcttgccgcg ccgcgcccgc tccgcgagca cgtgatactt
     3841 gcggcggaac ttgtcgaccg cagcgtcgtc gaggtcctcg agatccagta tcgcgttgtg
     3901 cgcgcctttc gtcgcgcgaa tcagctcgtc gagcttgatt tgaatcgctt ccgtgtcgcg
     3961 gttctgcgtg ttctgaatca aaaacaccat caggaacgtc acgatcgtcg tcgccgtgtt
     4021 gatcacgagc tgccacgtat cgctgaagtg gaacaacgct ccgctgatcg cccaaacgac
     4081 gatgatcgcg atcgaggcgg cgaacacatg cggccgcccg caaaagcgcg cggcgtcctt
     4141 ggcgaatctc gagtaccagt tgcgcgaatg catgcgttgc tcctcttcct ttggaagacg
     4201 caactaggat cgcgcagtgc tcgcggtctc gccagctgcg tccctagcag tgtcgcgtcg
     4261 agcgcttgaa ggcgttgagc ctggcgctca gactccgttg ggtacggcag tcgaggtacc
     4321 tcgtatgcgg cacgccatcg ttccggcagt cgtctcgggc ctgctcgccc tctccgtcgc
     4381 gcgcgcgcaa ccgagcggcg cgccggctgc ggagtcggac gcgcggctcg cggcgttgaa
     4441 gagcgccgcc atcgccgagg tcgagcagcg gcgcacgctg acgcagcaga tcgtcgacag
     4501 cctgttcagc ttcagcgagc tcggctacca ggagttcgag acgcagcggt atctgaccgc
     4561 gctgctcggg cgcaacggct tcacggtcga gcgcggggtt gccagcatcc cgtcgtcgtg
     4621 ggtcgcgcgc tggggcaatg gccgccccgt gattgcactc ggcgctgacg tcgacggctt
     4681 gccgacgacg aaccagacgc cgggcgtatt gacgcgacgc gagctcgttc cgggcgcgcc
     4741 gggacatggc gaaggccaca actcgggccc ggcgctcgtg atcacggcgg cgctcgccgc
     4801 gaaggccgtc atggagcgcg agcgtctgcc cggcacgctc gtcgtctggc ccggcatcgc
     4861 cgaggagctg ttggccggca aagcgtactt cgtccgcgcg ggagtgttcg ccgacgtcga
     4921 tgccgttctc tatgcccacg tcaactcggt gcttgccacg gaatggggag acgccgacac
     4981 gagcagcgcc gtatccgtcg agtacacgtt cacgggccag agcgcacatg cggcacgcgc
     5041 gccgtggctc ggccgtagcg cgctcgacgc ggtcgagctc atgaacgtcg gctggaacta
     5101 ccggcgcgag catctgcgac tcgcgcagcg ctcgcactat gtcgtcagca acggcggcga
     5161 ccagccgaac gtggtgccgc cgctggcctc cgtctggtac tacttccgcg agaacgattt
     5221 cgcgcacgtg caagatctct ggacgatcgg caaccag
//
LOCUS       DATLEE010000751         7752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11153633, whole genome shotgun sequence.
ACCESSION   DATLEE010000751 DATLEE010000000
VERSION     DATLEE010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11153633"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VM692_16510"
     CDS             complement(<1..290)
                     /locus_tag="VM692_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53833.1"
                     /translation="MSKRTVAILLAAFVVLLALVLFGQRQSASPAGAGAPLIAGLNDA
                     LADVERVTVTKANGETVATLEKRTDNWVVSDKHGYVANAAKLRQALTALAEAR"
     gene            complement(287..2200)
                     /locus_tag="VM692_16515"
     CDS             complement(287..2200)
                     /locus_tag="VM692_16515"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HUQ53834.1"
                     /translation="MSARSRKTYGYTTLALIALAFVVAVVATNAWVRGVRIDLTENNL
                     YTLGEGTQAVLAGIEEPINLYLFFSDEATATLPALRAYAVRVREMLEEFAANSDGKLV
                     LNVVDPVPFSEDEDRAEQFGLQAANVGPAGEAVYFGLAGSNSVGATDTIPFFQPDPRK
                     EAFLEYDLARLVYNLATTDKAVVGLLTAAPIGGGFNPQTQQPSQPWVVVDQAKQLLEV
                     RTLPPSVLSIDKDVDVLWLVHPQMLDDSTLYAVDQFIMRGGRALIFVDPVAEILAGED
                     PTGLGIGGAASSSTLERLFGAWGVNFNTMSVVTDNRYGLSIGGRFQPVRHIGLIGLDG
                     EAMRQDDPITSGLSSVNLGIAGYFELADGATAKLTPLLTSSVESEAMPADRFQFLPDP
                     ADLLKGFTPSGKQYTLAARLEGPLKSAFPDGAPARPDSPDNPNGQQPIDAALSAAHLA
                     ATDNANLVLVGDVDILSDRLWVQAQNFLGQRLVTAFANNGDFVINALDNLSGSAALIG
                     LRSRATYTRPFTTVEQLRRRADLQFRETEQRLQAELTQTEQRLGELQQARGDASNSLL
                     MSPEQQAEIQRFLDQQVRIRQELRAVRRNLDADIDRLGTELKVINIALVPVLLTILAL
                     TVVVVRRRRKSAS"
     gene            complement(2205..2939)
                     /locus_tag="VM692_16520"
     CDS             complement(2205..2939)
                     /locus_tag="VM692_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ53835.1"
                     /translation="MSGVMAVFKRELASYFATPIAYVFIVIFLVLAGVFTFFYGGFYE
                     TGQADLAGFFYWHPWLYLFLVPALSMRLWAEERKSGSIELLMTLPLETGQAVAGKFLA
                     AWAFTAISIALTFPIWITVNYLGDPDNGVIVAAYLGSILMAGGFLAVGACISATTKNQ
                     VIAFILSVVACFLLLAPGLGVVQQLLSGIAPQWLIDGLASLGFQAHFDYISKGVIDLR
                     DLVYFALLIGGFLFANTIVLELRKAD"
     gene            complement(2936..3871)
                     /locus_tag="VM692_16525"
     CDS             complement(2936..3871)
                     /locus_tag="VM692_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ53836.1"
                     /translation="MIEVQGLTKEYGRFRAVDDLSFRVAPGEVLGFLGPNGAGKTTTM
                     RIVAGFLPPTTGRVLVCGADIETQAVQAKRRIGYLPEGAPSYPEMTPRSFLEFIAEAR
                     GLAPAERRKRLDEVFALLHLDRVLEQSIDTLSKGYRRRVGLAQAILHDPEVLILDEPT
                     DGLDPNQKHEVRELIGRMARSKIIVISTHLLEEVEAVCNRAIIIARGKILADDTPRGL
                     EARSRFFNAVSLKLTNPDLIPAARNAIKLLPEVASTELDEKNGRITAFPAPGKLPLAP
                     INALAERQHWPLAELHLESGRLDEVFRSITGGAAA"
     gene            complement(3971..6703)
                     /locus_tag="VM692_16530"
     CDS             complement(3971..6703)
                     /locus_tag="VM692_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUQ53837.1"
                     /translation="MKRLLAVAAVAISLATAVVAQDPEIPFDEFYLDNGLRVVVHEDR
                     KAPIVAITIWYHVGSKNEVPGKTGFAHLFEHLMFNGSENHDDDYFRPLQEVGVTNING
                     TTDFDRTNYFETVPTSALDLALWLESDRMGHLVGAIDQDKLDEQRGVVQNEKRQGDNE
                     PYGKVFETILTNLFPPGHPYSWETIGSMEDLNAASLEDVKKWFETYYGPNNATLVLAG
                     DIDVATARDKVQRYFGDIPPGPPLTHKQRFIPPNVPSSRIVMEDRVPEARIYKVWLAP
                     EWISDDSTYLQLADYVLTSGKTSRLYRRLVYEDQIATDASAFAMINEIAGAYVVYATA
                     AEGQDLATVERALDEELERFLADGPTRAELDRVKTELRSGFIRGVEQVGGFRGKSNIL
                     AENAVYGGRPDFYKHSLELTNTATPQQVLQTARKWIRGEPLVIEVHPYATGLAPDSTS
                     VDRSTMPMPQTFPEAPFPALQQATLANGMRLIVAERHAVPVVQLSLQLDAGYAADQFA
                     SPGVATMTMEMLDEGTAEMDALQISDSLATLGAELSSGANLDFSVVGLSALKENLDES
                     LAIYSDVILNPAFPPAELERLKRLQLAGIQQEKNSPTDMALRVLPRLLYGDGHAYSLP
                     MTGSGTEEAVSALDRDDLVAYHDTWFKPNNATMIVVGDTTLAEIQPKLEALFARWRRG
                     DVPAKTLPDVAVPQRPSVYLIDRPGSEQSVIIAGHLIGKRDRADDIAVDAMNDILGGA
                     FTSRVNMNLREDKGWSYGADTTVVQTQAQRPFLAIAPVQADQTAASMREIKREIEELV
                     GARRATNDEVATSKRQSTLTLPGRWETARAVARDIAELVRFRLPDDYWNGYAELVNAL
                     DADEVNAAATRLLHPESMTWVVVGDRSVIEQDVRALGFGDLAIVDADGKTQASP"
     gene            6790..7746
                     /gene="pip"
                     /locus_tag="VM692_16535"
     CDS             6790..7746
                     /gene="pip"
                     /locus_tag="VM692_16535"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HUQ53838.1"
                     /translation="MSTRAQYPPIEPYDSGYLEVGDGHEIYYEQCGNPTGKPALFVHG
                     GPGGGGDVNARRFFDPAGYRIVVFDQRGSGRSRPHASLEANTTWHLVADMERLRGHLS
                     VDRWLVFGGSWGSTLALAYAETHPGAVSELVLRGIFLLRPLELTWFYQFGAGLLFPEQ
                     WQKFLAPIPPAERHDMLAAYHRRLLSSDAKARLEAAQAWSVWEGATSSLLPNPKREDQ
                     FGAPEFALALARIEAHYFVNRGFFTGDNQLLDGVEKIRKIPGVIVHGRYDVVCPIDTA
                     WELHRRWPEADLRIVRDAGHSAYEPGITAELIAATDRFLATA"
BASE COUNT         1279 a         2456 c         2640 g         1377 t
ORIGIN      
        1 cgagcctcgg cgagcgccgt cagcgcctgg cgcagcttgg ctgcgttcgc gacgtagccg
       61 tgcttgtcgc tgactaccca gttgtcggtg cgcttctcga gcgtcgcgac cgtctcgccg
      121 ttcgctttgg tcacggttac gcgctcgacg tcggcgagcg cgtcgttgag ccccgcgatc
      181 aacggagcgc ccgcgccggc cggtgacgcg ctctggcgtt gtccaaacag cacgagcgcc
      241 agcagcacga cgaaggccgc gagcagaatc gcgacggtac gcttgctcat gacgccgact
      301 tcctgcggcg gcgaaccacg acgacggtca gcgcgaggat cgtcagcaac accggcacca
      361 gcgcgatgtt gatgactttg agctccgtgc cgagacggtc gatgtcggca tcgaggttac
      421 ggcgcacggc acgcagctcc tgccgaattc gcacttgctg gtcgaggaag cgttggatct
      481 cggcctgctg ctcggggctc atcagcaacg agttgctggc gtcgccgcga gcctgttgca
      541 gctcgcccaa gcgctgctcc gtctgcgtga gctcggcctg aaggcgttgc tcggtctcgc
      601 ggaactgcaa atcggcgcgg cggcgcagct gctcgacggt cgtgaagggc cgcgtgtaag
      661 tggcgcggct tcgaaggccg atcagcgcgg cgctgccgga gaggttgtcg agcgcgttga
      721 tgacgaagtc gccgttgttc gcgaacgccg tcacgagccg ctgacccaag aagttctgtg
      781 cttgcaccca gaggcgatca ctcaagatgt cgacgtcccc gacgagcacg agattcgcgt
      841 tgtcggtcgc cgccaagtgc gcggccgaaa gtgcggcgtc gatcggttgt tggccattgg
      901 ggttgtcggg actgtccgga cgcgccggag cgccgtccgg gaatgccgac ttcagcggcc
      961 cttcgaggcg tgcggcgagc gtgtactgct tgccgctcgg cgtgaagcct ttcaggaggt
     1021 cggccgggtc cggtaagaat tggaagcggt cggccggcat ggcctcggac tcgacgctcg
     1081 acgtcagcag cggcgtcagc ttggccgtgg ccccgtcggc gagctcgaag taacccgcga
     1141 tgccgagatt caccgagctc aagcccgatg tgatcggatc gtcctggcgc atggcttcgc
     1201 cgtcgaggcc gatcaggccg atgtggcgaa cgggctggaa gcggccgccg atgctgaggc
     1261 cgtaccggtt gtcggtcacg acgctcatcg tgttgaagtt gacgccccac gcgccgaaca
     1321 gccgctcgag cgtcgagctc gacgcggcgc cgccgatccc gagccccgtg ggatcttcac
     1381 cggccaaaat ctcggcgacg ggatcgacga aaatcaacgc gcggccgccg cgcatgatga
     1441 attgatcgac ggcgtacagc gtgctgtcgt cgagcatctg cgggtgcacg agccagagca
     1501 cgtcgacgtc cttgtcgatc gagagcacgc tcggcggcag tgtccggacc tcgagcagct
     1561 gcttcgcttg gtcgacgacg acccagggtt gcgacggctg ctgcgtttgc gggttgaagc
     1621 cgccgccgat cggcgccgcc gtcaagaggc cgacgacggc cttgtcggtc gtcgcgaggt
     1681 tgtagacgag acgcgcgagg tcgtactcga ggaacgcctc cttgcgcggg tccggttgga
     1741 agaacggaat cgtgtcggtc gcgccgacgc tgttcgagcc cgccaagccg aagtagacgg
     1801 cctcgccggc ggggccgacg ttggccgcct ggagcccgaa ttgctcggcg cggtcctcgt
     1861 cctcggagaa cggcacgggg tcgacgacgt tgagcacgag cttgccgtcg gagttcgctg
     1921 caaattcctc gagcatctcg cggacgcgca cggcgtaggc gcgcagcgcc ggcagcgttg
     1981 ccgtggcttc gtcggagaag aacagataga gattgatcgg ctcctcgatg ccggcgagca
     2041 cggcctgcgt gccctcgccg agcgtgtaga gattgttctc cgtgaggtcg atccgcacgc
     2101 cgcgcaccca ggcgttcgtg gcgacgacgg cgacgacgaa cgcgagcgcg atgagcgcca
     2161 gcgtcgtgta gccgtaagtt tttctgctcc ttgcgctcat ggcttcagtc cgctttccta
     2221 agctcgagca cgatcgtgtt ggcaaacagg aaaccgccga tcaacagcgc gaaatagacg
     2281 agatcgcgca ggtcgatgac acccttgctg atgtagtcga agtgcgcttg gaaaccgagg
     2341 ctcgccagcc cgtcgatgag ccactgcggc gcgattcccg acaaaagctg ctgcacgacg
     2401 ccgaggcccg gcgccagcag caggaagcac gctacgaccg agagaatgaa ggcgatgact
     2461 tggttcttcg tcgtcgccga gatgcaagcg ccgacggcca ggaagccgcc cgccatcaga
     2521 atgctgccca agtaggccgc gacgatcacg ccgttgtcgg ggtcgcccag ataattgacc
     2581 gtgatccaga tcggaaaggt cagcgcgatg gaaatggccg tgaaggccca cgcggcgaga
     2641 aatttgccgg cgacggcttg cccggtctcg agcggcagcg tcatcaggag ctcgatgctg
     2701 ccggattttc gctcctcggc ccacagccgc atgctgagcg cgggcacgag gaacagatat
     2761 agccacgggt gccaatagaa gaaaccggcg agatccgcct ggcccgtctc gtagaagccg
     2821 ccgtagaaaa acgtgaacac gccggccagc accaagaaga tcacgatgaa cacgtacgcg
     2881 atcggcgtcg cgaagtagct cgcgagctcg cgcttgaaaa ccgccatcac gccgctcatg
     2941 ccgccgctcc gcccgtgatc gaccggaaca cctcgtcgag gcggccggac tcgaggtgca
     3001 gctcggccag cggccagtgc tgccgctcgg ccagagcgtt gatcggcgcg agcggcaact
     3061 taccgggcgc cggaaacgcc gtgatgcggc cgttcttctc gtcgagctcg gtgctcgcga
     3121 cttcgggcaa cagcttgatg gcgttgcgcg ccgcgggaat gaggtcggga ttcgtcagct
     3181 tcagcgacac cgcgttgaag aaccgcgagc gcgcctcgag gccgcgcggc gtgtcgtcgg
     3241 cgaggatctt gccgcgcgcg atgatgatgg cgcgattgca gacggcttcg acttcctcga
     3301 gcaggtgcgt cgagatcacg atgatcttcg atctggccat gcggccgatg agctcgcgta
     3361 cctcgtgctt ctggttcggg tcgagcccgt ccgtcggctc gtcgaggatc aagacctcgg
     3421 ggtcgtgcag gatcgcctgc gcgaggccga cgcggcggcg atagcctttc gacaacgtat
     3481 cgatgctctg ctcgagcacg cggtcgagat gcagcagcgc gaacacctcg tcgagccgct
     3541 tgcgccgctc ggcgggcgcg aggccgcgcg cctcggcgat gaactcgagg aacgagcgcg
     3601 gcgtcatctc gggatagctc ggcgcacctt ccggcaggta gccgatgcgc cgcttggctt
     3661 ggacggcctg cgtctcgatg tcggctccgc agacgagcac gcggcccgtc gtcggcggca
     3721 agaaccctgc gacgattcgc atcgtagtcg tcttaccggc gccattcggc cccaggaagc
     3781 cgagcacctc gcccggcgcg acccggaagc tcagatcgtc tacggcgcgg aaccgaccgt
     3841 attctttcgt taggccctga acttcgatca ttggaagagc tcggcggcag ttgttgttgc
     3901 aacgggttgg ccgccgagcg gcgggcacat tctgctatac agctaaaaac gctttcaagt
     3961 tacagaacgt tcatggactg gcttgcgttt tgccgtcggc gtcgactata gccaggtcgc
     4021 cgaagccgag cgcgcgcacg tcttgctcga tgacgctgcg atcgccgacg acgacccagg
     4081 tcatcgactc ggggtgcagc agccgcgtcg ccgcggcatt gacctcgtcc gcgtcgaggg
     4141 cgttcacgag ctcggcatag ccgttccagt agtcgtcggg caagcggaag cgcacgagct
     4201 cggcgatgtc gcgggcgacc gcgcgcgccg tctcccagcg tcccggcaga gtcagcgtgc
     4261 tctggcgttt gctcgtcgcg acctcgtcgt tcgtggcgcg acgcgcgccg acgagctctt
     4321 cgatctcgcg tttgatctcc cgcatcgacg ccgccgtctg atcggcctgc acgggcgcga
     4381 tcgcgaggaa cggccgttgc gcttgcgtct gcacgaccgt cgtgtcggcg ccgtacgacc
     4441 agcctttgtc ctcgcgcaag ttcatgttga cgcgcgacgt gaacgcgccg cccaaaatgt
     4501 cgttcatcgc gtcgaccgcg atgtcgtcgg cgcgatcgcg tttgccgatc aagtgacccg
     4561 cgatgatgac gctctgctcg gaccccgggc ggtcgatcag gtagacgctc gggcgctggg
     4621 gcactgcaac gtcgggcagc gtcttggcgg gcacgtcgcc gcggcgccag cgagcgaaca
     4681 acgcctcgag cttcggctgg atctcggcga gcgtcgtgtc gccgacgacg atcatcgtgg
     4741 cgttgttcgg cttgaaccaa gtgtcgtgat aggcgacgag atcgtcgcgg tcgagcgcgc
     4801 tgacggcctc ctcggtgccc gagccggtca tcggcagcga gtaggcatgc ccgtcgccgt
     4861 agagcagcct tggcagcacg cgcaaagcca tgtccgtcgg ggagttcttt tcctgctgaa
     4921 taccggcgag ctgcaagcgt ttgaggcgct cgagctcggc gggcgggaag gccgggttca
     4981 gaatgacgtc ggagtagatc gccagcgatt cgtcgaggtt ctccttcagc gccgacaggc
     5041 cgacgacgga gaagtcgaga ttcgcgcccg aggacagctc ggcgccgagc gtcgccagcg
     5101 agtcgctgat ctgaagtgcg tccatctcgg ccgtgccctc gtcgagcatc tccatcgtca
     5161 tcgtcgcgac gccgggtgag gcgaattggt cggccgcata gccggcgtcg agctgcaagc
     5221 tcagctgcac gacgggcacg gcgtggcgct cggcgacgat cagccgcatg ccgttcgcga
     5281 gcgtcgcttg ctgcaaggcc gggaacggcg cctcggggaa cgtctgcggc atcggcatcg
     5341 tcgagcgatc gacgctcgtg ctgtcgggcg ccaatcccgt cgcgtacggg tggacctcga
     5401 tgacgagggg ctcgccgcga atccacttgc gcgccgtctg cagcacttgc tgcggcgtcg
     5461 ccgtgttcgt gagctcgagc gaatgcttgt agaagtccgg ccgaccgccg tagacggcgt
     5521 tctcggcgag gatgttcgac ttgccgcgga agccgccgac ttgctcgacg ccgcgaatga
     5581 acccgctgcg cagctccgtc ttcacgcggt cgagctcggc gcgcgtcggg ccgtcggcga
     5641 ggaagcgctc gagctcctcg tcgagcgcgc gctcgacggt cgcgaggtct tggccttcgg
     5701 cggccgttgc atagacgacg taggcgcccg cgatctcgtt gatcatcgcg aacgcgctcg
     5761 cgtcggtcgc gatctggtct tcgtagacga gccgtcgata gagccgcgac gtcttgccgc
     5821 tcgtcagcac gtagtcggcg agctggaggt aggtcgagtc gtcgctgatc cactcgggtg
     5881 ctagccacac cttatagata cgcgcctcgg gcacgcggtc ctccatgacg atgcggctcg
     5941 acgggacgtt cggcggaatg aagcgctgct tgtgcgtcaa cggcgggccc ggcgggatgt
     6001 cgccgaagta gcgctgcacc ttgtcgcgcg cggttgcgac gtcgatgtcc ccggcgagta
     6061 cgagggtcgc gttgttcggc ccgtagtacg tctcgaacca cttcttaacg tcctcgaggc
     6121 tcgcggcgtt caggtcctcc atggagccga tggtttccca cgagtacggg tgtcccggcg
     6181 gaaagagatt cgtgaggatc gtctcgaaca ccttgccgta gggctcgttg tcgccttgcc
     6241 gcttctcgtt ctgcacgacg ccgcgttgct cgtcgagctt gtcttggtcg atggcgccga
     6301 cgaggtgccc catgcgatcc gattcgagcc acagcgccag atcgagggcc gaggtcggca
     6361 cggtctcgaa gtaattcgtg cggtcgaaat cggtcgtgcc gttgatgttc gtgacgccga
     6421 cctcctgcag cgggcgaaaa tagtcgtcgt cgtgattctc gctgccgttg aacatcagat
     6481 gctcgaacag gtgcgcgaag ccggtcttgc cgggcacttc gttcttcgag ccgacgtgat
     6541 accaaatcgt gatggcgacg atcggcgcct tgcggtcctc gtgcacgacg acgcgcaggc
     6601 cgttgtcgag atagaactcg tcgaacggta tctcggggtc ctgagcgacg acggccgtcg
     6661 ctagactgat ggcgacagcg gcgaccgcaa gtaagcgttt catcggagtt ccgtgcgcag
     6721 tggatcgagt agagtgagta taagcgcgga cccaaaacgc ctggcacacg aggacattac
     6781 gagaccgtca tgagcacgcg cgcgcagtat ccgccgatcg agccctacga cagcggctac
     6841 ctcgaagtcg gcgatgggca cgagatctac tacgagcagt gcgggaatcc gacgggcaag
     6901 ccagcgctat tcgtgcatgg cgggcccggt ggcggcggcg acgtcaacgc acgccgcttt
     6961 ttcgacccgg ccggttatcg catcgtcgtg ttcgatcaac gcggctcggg gcgcagccgt
     7021 ccgcacgcct cgctcgaggc caacacgacc tggcacctcg tcgccgacat ggaacgcttg
     7081 cgcggccact taagcgtaga tcggtggctc gtcttcggcg ggtcttgggg cagcacgctg
     7141 gcactcgctt atgccgagac gcaccccgga gcggtcagcg agctcgtgct gcgcggcatt
     7201 tttctgctgc gcccgctcga gctgacgtgg ttctatcagt tcggcgcggg gctcttgttt
     7261 ccggagcagt ggcaaaaatt cttggcgccg atcccacctg cggagcgtca cgatatgctc
     7321 gccgcctatc atcggcgttt gctgtcgagc gatgcgaagg cccgcctcga agcggcacag
     7381 gcgtggtcgg tctgggaagg agccacgagc tcgctgctgc cgaacccgaa gcgcgaagac
     7441 cagttcggcg cgccggagtt cgcgctggcg ctcgcgcgca tcgaggccca ctatttcgtc
     7501 aatcgcgggt tctttaccgg cgacaatcaa ttgctcgatg gagtagagaa aatccgcaag
     7561 attccgggcg tgatcgtgca cgggcgttac gacgtcgtct gcccaatcga cacggcgtgg
     7621 gagctacacc ggcgttggcc cgaggcggat ttgcggatcg tgcgcgacgc cggtcactcg
     7681 gcctacgagc ccggcatcac ggcggagctc atcgcggcga ccgatcgatt cttggcgaca
     7741 gcgtaggagc cg
//
LOCUS       DATLEE010000752         5520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10234959, whole genome shotgun sequence.
ACCESSION   DATLEE010000752 DATLEE010000000
VERSION     DATLEE010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10234959"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /gene="rseP"
                     /locus_tag="VM692_16540"
     CDS             complement(<1..564)
                     /gene="rseP"
                     /locus_tag="VM692_16540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HUQ53839.1"
                     /translation="MDFSILERALWFIVAISVLVAAHEFGHFIVARTLGFKVLRFSIG
                     FGRPLLAWRGGPPDHIQYWLSMIPLGGYVKMLDEHEGPVSSAERHRAFHQRPVWQRVA
                     VLLAGPAFNFLFAIIAYWLMFVSGVQALKPLVAGVEPSSVAASAGLNPGDEIEAIGGQ
                     TTRTWEDGILRIFEELLDDGRIELSVRD"
     gene            complement(711..1505)
                     /locus_tag="VM692_16545"
     CDS             complement(711..1505)
                     /locus_tag="VM692_16545"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HUQ53840.1"
                     /translation="MLAARIATALVFGAAITAAFLYAPPPVTAAVLALLWLAGVWEWA
                     AFAKLPGSLSVAYTTLFAAAMAVAWWLNGEGLAVLLVVALLWWLFALVLVVRYPRSFT
                     PTFVALAGIVVLLPSWALLVRLHREGALGAELAFTLLLIVWAADVGAYAFGRLLGRTK
                     LAPAVSPGKTWEGVTGGLLTAGLAAGLAAPWLDLPVSRLVVLGVATALISVLGDLTQS
                     MFKRNVGLKDSGKLLPGHGGVLDRIDSLTAAVPAFVVGLWALNLMG"
     gene            complement(1525..2082)
                     /gene="frr"
                     /locus_tag="VM692_16550"
     CDS             complement(1525..2082)
                     /gene="frr"
                     /locus_tag="VM692_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HUQ53841.1"
                     /translation="MINDIKKAGEQRMQRGVEAFKEDLRKLRTGRAHAGLLETVMVEY
                     YGTNVPIKQTANISVEDARTIVVTPWEKNMVAAVEKAIINSGLGLNPVTAGQVIRVPL
                     PPMTEERRRDLVKVVRAHAETARVGLRNVRREALGDLKELLKEKMIAEDDERRGQEEI
                     QKLTDRYVANIDGLLKEKEAEIMEF"
     gene            complement(2079..2828)
                     /gene="pyrH"
                     /locus_tag="VM692_16555"
     CDS             complement(2079..2828)
                     /gene="pyrH"
                     /locus_tag="VM692_16555"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HUQ53842.1"
                     /translation="MAQRSPVYKRILLKLSGEALLGSQHYGIDPAVIRRVAIEVSEVV
                     GLGVQVGLVIGGGNIFRGAGLAEAGMDRVTADQMGMLATVINALAMQDALERQGTGVR
                     VMSALQIHEVCEDYIRRRAVRHLEKGRVTVFAAGTGNPFFTTDTAASLRAIEIGADVL
                     IKATKVDGVYSADPVKDPKAVRYPALSFDQVLSDDLRVMDATAIVMCRENNLPLVVFN
                     LNNAGDLVRLITGEAVGTSVMNHLPRTGTGK"
     gene            complement(2997..3875)
                     /gene="tsf"
                     /locus_tag="VM692_16560"
     CDS             complement(2997..3875)
                     /gene="tsf"
                     /locus_tag="VM692_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HUQ53843.1"
                     /translation="MDIKPALVKELRERTGAGMMECKKALVEAQGDIDAAADLLRKAG
                     AAKADKKSSRVAAEGRVAIRSDEKAGRHVVLEVNSETDFVAKDENFRAFVEKLADVVL
                     SQRPANVEALLRSTVDGRTLEELRLELVTKVGENISVRRFDVVESSGTVGTYVHGTRI
                     GVLVEIEGGNVALARDLAMQVASLAPRYVAGEDVPKEIVDKEREIIVAQTAEEKKPPE
                     ILAKMVEGKVRKFLDEITLTGQVFVKDDKKRVRDVLAANKAKVTTFRRYEVGEGIEKK
                     TTDFRAEVMAQASQIR"
     gene            complement(3924..4733)
                     /gene="rpsB"
                     /locus_tag="VM692_16565"
     CDS             complement(3924..4733)
                     /gene="rpsB"
                     /locus_tag="VM692_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HUQ53844.1"
                     /translation="MADVTMRRMLEAGVHFGHQTRFWNPKMAEFIFGERNKIHIINLD
                     KTLPSYQAAAHFVRKMVADGGTLLFVGSKRAAREAMQAEATRCGMPYVTQRWLGGMLT
                     NFKTIRQSIKRLKTLEEITEPGAQTSFTKKELLRFGREKEKLEKTLGGIKNMNGLPNA
                     VFLIDVGHEEIAVSEARKLGIPIVAIVDTNCSPEGIADAVIDGKASIPEVPRGDDEFV
                     ELDESGKPKVEARAKKAPPKKRGRVTKVRAKGRPRTGDEPAPEGEEEAESE"
     gene            4963..>5520
                     /gene="map"
                     /locus_tag="VM692_16570"
     CDS             4963..>5520
                     /gene="map"
                     /locus_tag="VM692_16570"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HUQ53845.1"
                     /translation="MITIKTPEEQDKMRVAGRLAAQVLDMIAPHVQPGIATEELDRLC
                     HEYIVGELASIPAPLNYRGSGGAAPFPKSICTSVNHVVCHGIPSDRVLRNGDIVNIDV
                     TVIKDGYHGDTSRMYMVGTPSIMAERLTRVCFDAMWRGIRAIRPGNHLGDIGHAIQTF
                     VEDQRFSVVREYCGHGIDRVFHEDPQ"
BASE COUNT          930 a         1863 c         1708 g         1019 t
ORIGIN      
        1 atcgcgcacc gacagctcga ttcggccgtc atcgagcagc tcctcgaaga tccgcaggat
       61 gccgtcctcc cacgtccgag tcgtctggcc gccgatcgct tcgatttcgt cgccgggatt
      121 gaggcccgcg cttgccgcaa cggagctcgg ctcgacgccg gccacgagcg gcttcagcgc
      181 ctgcactccg ctcacgaaca tcaaccagta cgcgatgatc gcaaacagga agttgaacgc
      241 gggccctgcg agcaatacgg ccacccgctg ccaaacgggg cgctggtgaa acgcacggtg
      301 acgctccgcc gacgagacgg gtccctcgtg ctcgtcgagc atcttgacgt agccgccgag
      361 cgggatcatc gacagccaat attgaatgtg atcgggcgga ccgccgcgcc atgcgaggag
      421 cggcctgccg aagccgatcg agaaccgcag gaccttgaag ccgagcgtac gggccacgat
      481 gaaatggccg aactcgtgcg ccgcaaccag cacgctgatc gcaacgatga accacaacgc
      541 tcgttcgagt atcgagaagt ccattggggc ggataccgcc ggtcctttgg cgacctgggc
      601 ttgagggtcg gtattctagc cggccggtcg tccgcggggc acgaccattt ggccgataac
      661 tcagtcgcta tgccgctaga gaccgcggca tcgcgccgac agttcccggg tcagcccatc
      721 aggttcaagg cccataggcc gacgacgaac gccgggacgg ccgccgtgag gctgtcgatg
      781 cggtcgagca cgccgccgtg cccgggcaac agcttgccgc tgtccttgag gccgacgttg
      841 cgcttgaaca tgctttgggt caggtcgccg agcaccgaga tcagtgccgt cgcgaccccg
      901 agaacgacga ggcggctgac ggggaggtcg agccatggag ccgcgagccc ggcggcgagc
      961 ccggcggtca gcaggccgcc cgtgacacct tcccaggttt tgccggggct gacggccggc
     1021 gcgagcttag tgcgaccgag cagtcgtccg aacgcgtagg cgccgacgtc ggcggcccac
     1081 acgatcagca gcagcgtgaa cgcgagctcg gcgccgagcg cgccctcgcg atgcaggcgc
     1141 acgagcaacg cccaggacgg cagcagcacg acgatgcccg cgagcgctac aaacgtcggg
     1201 gtgaaactac gcggatagcg caccacgagg accaatgcga acaaccacca gagcagtgcg
     1261 acgacgagca gcaccgccaa gccttcgcca ttgagccacc aggcgacggc catcgcggcg
     1321 gcaaacagcg tcgtataggc aacgctcaac gaaccgggca gcttcgcgaa agcggcccac
     1381 tcccagactc ctgccaacca aagcaatgcc aacacggcgg ccgtgacggg cggcggcgca
     1441 tacaaaaacg ccgcggtgat ggcggcgccg aagacgagtg cggttgcgat cctcgcggct
     1501 agcatggcgg agcgcttcgt gcgctcaaaa ctccatgatc tcggcttcct tttccttcag
     1561 cagcccgtcg atgttcgcga cgtagcggtc cgtcagcttc tggatctctt cttggccgcg
     1621 ccgttcgtcg tcctccgcga tcatcttctc tttgagcagc tctttgaggt cacccagcgc
     1681 ttcacgacgc acgttgcgta gcccgactct tgcggtctcg gcgtgcgcgc gcacgacctt
     1741 gacgaggtcg cggcgccgtt cttcggtcat ggggggcagc ggcacgcgga tgacctgccc
     1801 ggccgtgacc ggattcagcc caagacccga gttgatgatg gccttctcga cggccgccac
     1861 catgtttttc tcccacggag tcacgacgat cgtgcgggcg tcttccacgg agatgttggc
     1921 cgtctgcttg atgggcacgt tcgtgccgta gtactcgacc atgacggtct cgagcaggcc
     1981 ggcgtgcgcg cggccggtac gcagcttgcg caagtcttcc ttgaacgcct cgacgccgcg
     2041 ctgcatgcgc tgctcgccgg ccttcttgat gtcgttgatc acttgccggt cccggttcga
     2101 ggcagatgat tcatgaccga tgtcccgacg gcttcgccgg tgatcaagcg cacgagatcg
     2161 ccggcattat tgagattgaa gacgacgagc ggcaggttgt tctcgcggca catgacgatc
     2221 gcggtggcgt ccatgacgcg caagtcgtcg gacagcacct gatcgaacga caacgcaggg
     2281 tagcgaacgg ccttgggatc cttgacgggg tccgccgaat acacaccgtc gaccttcgtg
     2341 gctttgatca gcacgtccgc gccgatctcg atcgcgcgca ggctcgccgc cgtgtcggtc
     2401 gtgaagaacg ggttacccgt gccggccgcg aaaaccgtga cgcggccttt ttcgaggtgg
     2461 cgcacggcgc gccgcctgat gtagtcctcg cagacctcgt ggatttgcag cgccgacatg
     2521 acgcgcacgc cggtgccctg gcgctcgagc gcatcctgca tcgcgagcgc gttgatgacg
     2581 gtcgcgagca tgcccatttg atcggccgtc acgcggtcca tgccggcctc ggccaagccc
     2641 gcgccgcgga agatgttgcc gccgccgatg acgaggccga cttgcacgcc gagcccaacg
     2701 acctcgctga cctcgatggc gacacggcgg atgacggcgg ggtcgatgcc gtaatgctgg
     2761 ctgccgagca aagcctcgcc gctcaacttc agcaagatcc gcttatagac cggcgagcgt
     2821 tgcgccataa aacttcaact caaattaagg tgcgacccgg tgggctgtgc tcgcacggtt
     2881 tctcccgaga ccgttcggcg cacatggatg tgcgccgacg agccgcaggg atgctcttcc
     2941 ggcgtgtctc gggagagacc gtgcgagcgc agcacaccgc agcgatgtcg cctaatttag
     3001 cggatctgag aggcctgcgc catgacctcc gcgcggaagt cggtcgtctt tttctcgata
     3061 ccctcgccga cctcgtaacg gcggaaggtc gtcaccttcg ccttattggc ggcaagcacg
     3121 tcgcggacgc gcttcttgtc gtccttgacg aacacctgcc ccgtcaacgt gatctcgtcg
     3181 aggaacttgc gcactttgcc ctcgaccatc ttggcgagga tctcgggcgg cttcttctcc
     3241 tcggccgtct gcgcgacgat gatctcgcgc tctttgtcga cgatctcttt cggcacgtcc
     3301 tcaccggcga cgtaacgcgg cgcgagcgag gccacttgca tcgcgaggtc gcgcgcgagc
     3361 gcgacgttgc cgccctcgat ctcgacgagc acgccgatgc gcgtgccgtg cacgtaggta
     3421 ccgacggtgc cgctgctttc gacgacatcg aaacggcgca ccgagatgtt ctcgccgact
     3481 ttcgtcacga gctcgagccg cagctcctct agggtgcggc cgtcgacggt gctgcgcagc
     3541 agcgcctcga cgttcgcggg acgctggctc aagacgacgt ccgcgagctt ctcgacgaat
     3601 gcgcggaagt tctcgtcctt ggcgacgaag tcggtctccg agttgacctc gaggacgacg
     3661 tgccgcccag ccttctcgtc actacggatt gcgacgcggc cctcggccgc cacgcgcgac
     3721 gacttcttgt cggccttcgc ggcgccggcc ttgcgcagca agtccgcggc agcgtcgata
     3781 tcgccctggg cttcgaccaa ggctttcttg cattccatca tgccggcgcc ggtgcgctcg
     3841 cgcagctctt tgaccaaggc tggtttgata tccatacccc aagccctctc ctttaaatac
     3901 gtttcgacag tgcgccgctg cgactattcg ctctcggctt cctcttcccc ttcaggggca
     3961 ggctcatcgc cggtgcgggg gcggcctttc gcccgaacct tcgtgacgcg cccacgtttc
     4021 ttcggcggcg ccttcttggc acgcgcctcg accttcggct tgccgctctc gtcgagctcg
     4081 acgaactcgt cgtcgcctcg cggcacttcg gggatcgatg ccttgccatc gatcacggcg
     4141 tcggcgatgc cttccggcga gcagttcgtg tccacgatcg cgacgatggg gatgccgagc
     4201 ttgcgcgcct cgcttacggc gatctcttca tgccccacgt cgatcaggaa gacggcattc
     4261 ggcagcccat tcatgttctt gatgccgccg agcgtcttct cgagcttttc cttctcgcgc
     4321 ccgaatcgca gcagctcttt cttggtgaaa gacgtctgcg cgcccggctc cgtgatctct
     4381 tcgagcgtct tcaagcgctt gatcgactga cggatcgtct tgaagttcgt gagcatgccg
     4441 ccgagccagc gctgcgtgac gtacggcatg ccgcaacgcg tcgcctccgc ctgcatcgct
     4501 tcgcgcgcgg cgcgcttcga cccgacgaac agcaaggtgc cgccgtcggc gaccatcttg
     4561 cgcacgaagt gcgccgccgc ctgatagcta ggcagcgtct tgtcgaggtt gatgatgtgg
     4621 atcttgttgc gctcgccgaa gatgaactcg gccatcttgg gattccagaa acgggtttgg
     4681 tgcccgaaat gcacgcccgc ttccagcatc cggcgcatgg tgacgtcggc catggaatgc
     4741 tcctttgggg ttgatcctcc acgcgtttct gcggacgacc ccgttgccgg ggcaccccgc
     4801 ccgcagtatt tggcgcgtgt gtgtaattac ctcggcgctg gtttgcctcc gaggcgcgcg
     4861 ctttatacca tagccccctt ccggcaaaca acgtaaatcc gcgtgcgcgg cgccgttttg
     4921 ccgctcctaa cgcccaaccg cagcctcgta gcgacctcga cgatgattac catcaaaacg
     4981 cctgaagagc aggacaagat gcgcgtggcc gggcgtttgg cagcgcaggt gctcgacatg
     5041 atcgcaccgc acgtccagcc gggcatcgcc accgaggagc tcgaccggct ctgccacgag
     5101 tacatcgtcg gcgagctcgc ctccattccg gccccgctga actaccgcgg ctcgggcggg
     5161 gccgcgccgt tcccgaagtc gatttgcacg tcggtcaacc acgtcgtgtg ccacggcatt
     5221 ccgagcgacc gcgttctacg caacggcgac atcgtcaata tcgacgtcac cgtcatcaag
     5281 gacggttacc atggcgacac gagccgcatg tacatggtcg gcactccgtc gatcatggcc
     5341 gagcgtctga cgcgcgtgtg cttcgatgcg atgtggcgcg gcattcgcgc catccgcccc
     5401 ggtaatcatc tcggcgacat cggccacgcg attcaaacct tcgtcgagga tcaacgcttt
     5461 tcggtcgttc gcgaatactg cggccacggc atcgaccgcg tattccacga ggacccgcaa
//
LOCUS       DATLEE010000753         5686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11155837, whole genome shotgun sequence.
ACCESSION   DATLEE010000753 DATLEE010000000
VERSION     DATLEE010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11155837"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VM692_16575"
     CDS             complement(<1..954)
                     /locus_tag="VM692_16575"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUQ53846.1"
                     /translation="MRIRRSAAAFLLALVPQWLLAQDAGLPPRPLPDAPFVIDTAEAG
                     QVRVTPLKGLELPWDLAFVPSGDMLVTERTAMRLRVVRNGKLEPQAIAELPQGMASSG
                     GGGGLMAVAAHPRFAETRWVYLTYTKPAERGRRGFGLARGRLDGMELTDVRELFKVDE
                     AGGGPPPGLPLVFGRDGYLYFFVGGANDEIAQRGDSYYGKVLRFNDDGSVPSNPFAGR
                     EGFKPEIFTIGHRNGIGLTIHPTTGAVWESENGPYGGDEVNILKAGANYGWPLVSYGR
                     DYSGAPVSDKRTLPDTEDPVLFWVPSIAISGITFYTGDRFPA"
     gene            complement(1008..1505)
                     /locus_tag="VM692_16580"
     CDS             complement(1008..1505)
                     /locus_tag="VM692_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53847.1"
                     /translation="MTDATRSAGIERPHESASVARAAAKGAFAGLAATAAIALAGRRE
                     TGSAIAPINATSHVLWGDEAGATNAVDVKHTVTGLLLNGGAGVFWALVHELLLTRLAR
                     RDRAAAIASGAAVAGLAYVVDYHLVPRRLSPGWELRLSPRSVWLGFVALGAALSVAAL
                     MRARR"
     gene            1674..3704
                     /locus_tag="VM692_16585"
     CDS             1674..3704
                     /locus_tag="VM692_16585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUQ53848.1"
                     /translation="MKLRRLYIGITTALSDLGAAAAQNAPNSGPGGSVEEVVVTGARL
                     EQSLPQDLASVGNRLTVITDDQIRNGGYNDVSQVLQTLVPGLYLSPKQGPFDYVDAAL
                     QGSRTNEILWLVDGVRVSNRLYNSVTPFDTLPAHMIERIEILEGGQGLFYGTQAVSGV
                     VNVITKGFTEENQGEIGLGTDSHDGEHINAFFRGAIDANDKFVLFASSDQSQGYQPFP
                     DEDYQPSITDRHRGYDVTTIGGKFSHDFTDGLRLALGYQRIDADLDQDAPDTYLDAFN
                     ERQEDIFTGTLDFAASDTVQFYVKSYFHQWDSYWTEYDVDLDNPGDVVLIDHEAFWGF
                     KDYGLNALAKLSPGDAVEYYVGYDYQKYSGRDDVLLIAPNKESVHAVFGQVRTTPELF
                     DKARIAFGVRRNMPSESAAETVWNASGQYDFSQRLFARATVGTSFRLPDAYELFAVDT
                     TCCLGNPNLKPESSENYNVSLGGLLGDNSMMNWEVVYFRRYVKDLIVDVDLDDGSGLT
                     QAQNVDNEVRVRGEQVVLGFRPSDTISASVSYTHNDSEALNSNTTSGGYTEIPGIPKD
                     QLAASLNYEPTGKPFGLTLTLNDVGSVFDIVGGVGPVARGDYTVVDVAARLYLDDSQR
                     HKVMFRIENAFDETYTTRYRRSTVDGSTPPVRYAARNLGMPRTLHVSYVHSF"
     gene            3720..5312
                     /locus_tag="VM692_16590"
     CDS             3720..5312
                     /locus_tag="VM692_16590"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HUQ53849.1"
                     /translation="MSAIAPVNVGVRLGERTMDVLVTAHRWLGTALCLVFATWFASGI
                     VMMYAGYPLLSEGDKLARTPPLDLAAAQLTPAEAYRRAGLDSPVDYVRLGTMLDRPTY
                     FFLTGGSWVAVGADDGTVRPALDADAALGVARAFEVGEVAPPRYAGLFESPDQWTIYT
                     SWAPYLPGHDFSPFHKLEFDDGTEVSVLAATGDVIQRTTPSERLWGYLGAVVHWIYPT
                     TFRTRHVDAWNDLIVWLSALGTVLCVSGVAIGIYFFRWRREPGVAGSPYRGWMKWHHW
                     IGLVFGVLAGPWVFSGLMSMDPWAWSTTDDSPDGAQVDRMSGALDLDAFVAAPATAAA
                     ACRSQVNTKEIRLVQLAGVPYYYCSQAPNRSALVAADGSGAPPFESFAQSALVDAARA
                     ILPGAEIASAELLDHYDAYYYPGWEDKLENGAAKRLPVLKIAFDDVERTLVYVDPYTA
                     VPVKVYDTSGRWYRWLFHGLHSLDFGALYRSRPLWDLLVLPLMLGGVAVSVTGAWIGY
                     KWLKRKVRKPKPQKRRTAPVAP"
     gene            5323..>5686
                     /locus_tag="VM692_16595"
     CDS             5323..>5686
                     /locus_tag="VM692_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUQ53850.1"
                     /translation="MLEAIGVSKRFGDKLALDDVTLRVEPGEIYCLLGANGAGKTTLI
                     NVFLNFLAPSEGEARIDGLDVGRHPLQTKQRVAYIPEHVMLYGVLSGLENLRYFAALG
                     TGKKASRGELLDLLATVGL"
BASE COUNT          963 a         1919 c         1882 g          922 t
ORIGIN      
        1 ggccgggaag cgatcgcccg tgtagaacgt gatgccggaa atcgcgatcg acggcaccca
       61 gaacagcacg gggtcttcgg tgtccggcag cgtccgcttg tcggacacgg gcgcgccgct
      121 gtagtcgcgg ccgtagctga cgagcggcca gccgtagttc gcgccggcct tcaggatgtt
      181 gacctcgtcg ccgccgtacg ggccgttctc gctctcccag accgcacccg tcgtcggatg
      241 aatcgtcagg ccgatgccgt tgcgatgccc gatcgtgaag atctccggct tgaagccctc
      301 gcggcccgcg aacggattgc taggcacgct cccatcgtcg ttgaagcgca ggaccttgcc
      361 gtagtacgaa tcgccgcgtt gcgcgatctc gtcgttcgca ccgccgacga agaagtagag
      421 atagccgtct cgcccgaaca cgagcggcag ccccggcggc gggccgccgc cggcctcgtc
      481 gaccttgaag agctcgcgga cgtccgtgag ctccatgccg tcgagacgcc cgcgcgcgag
      541 cccgaagccg cggcggccgc gctccgcagg tttcgtgtaa gtcaggtaca cccaacgcgt
      601 ctcggcgaag cgcgggtgcg ccgcgacggc catgagcccg ccgccgccac cgctgctcgc
      661 cattccctgt ggaagctcgg cgatcgcttg cggctcgagc ttgccgttac gcacgacgcg
      721 caaccgcatg gcggtccgct ccgtgacgag catgtcgccg ctcggcacga acgcgagatc
      781 ccacggcagc tcgaggccct tcagcggcgt cacgcgcacc tgacccgctt cggcggtgtc
      841 gatgacgaac ggtgcatcgg gcagcggccg aggcggtaag ccggcgtctt gagccaacag
      901 ccattgcgga acgagagcga ggaggaacgc ggcggcggaa cggcgaatgc gcacgaggcg
      961 aggctccgat cgaaaggacc ggccgagaag gatagtcgtt gcgcgcgtta gcggcgagcg
     1021 cgcatcaacg ccgcgacgct caaggccgcg ccgagcgcga cgaagcccag ccaaacggag
     1081 cgcggcgaga ggcgcagctc ccagcccggc gacagtcggc gcggtacgag atgatagtcg
     1141 acgacgtagg cgaggcccgc gacggccgca ccgctcgcga tggcggccgc gcggtcgcgg
     1201 cgcgccagcc gcgtcagcag cagctcgtgc accaacgccc agaacacacc ggcgccgccg
     1261 ttcaggagca ggcctgtaac cgtgtgtttc acgtcgacgg cgttcgttgc accggcttcg
     1321 tcgccccaga gcacgtggct cgtcgcgttg atgggcgcga tggcgctccc cgtctcgcgg
     1381 cggcccgcca gcgcaatggc tgctgtcgcg gccaagccgg cgaacgcgcc tttggccgcc
     1441 gcccgcgcca ccgaagccga ttcgtgtggc cgctcgattc ccgccgatcg ggtcgcatcc
     1501 gtcattgcat tgtccttgct aacgctggcg caaacggctg caagacccgt gcccctcgct
     1561 gggttaacgg ttaactaacg accggctaac ccgctaaaca cctgaataac ctaccgccgg
     1621 ccggcgggct ttgtgagact cctgcgcctc acgaccactt accgagaaca actatgaagc
     1681 tgcgacgcct atatattgga atcaccacgg ccctctcgga cctcggcgcc gctgccgcgc
     1741 agaacgcgcc gaatagcgga ccgggtggtt cggtcgagga agtcgtcgtg acgggcgcac
     1801 ggctcgagca gtcgctgccg caagacctcg cgagcgtcgg caatcgcctc accgtcatca
     1861 cggacgacca aattcgcaac ggcggctata acgacgtctc gcaagtcctg cagacgctcg
     1921 tgccggggct ctacttgtcc ccgaagcaag ggccgttcga ctatgtcgac gcggcgctgc
     1981 aagggtcgcg cacgaacgaa attctgtggc tcgtcgacgg cgtgcgcgtc agcaaccgcc
     2041 tctacaacag cgtgacgccg ttcgacacgc tgccggcgca catgatcgag cgcatcgaga
     2101 tcctcgaagg cgggcagggc ctgttctacg ggacgcaagc cgtctcgggc gtcgtcaacg
     2161 tcatcacgaa aggttttacg gaggagaacc aaggcgaaat cgggctcggt accgacagtc
     2221 acgacggcga gcacatcaac gccttcttcc gcggcgcgat cgacgccaac gacaagttcg
     2281 tgctgttcgc gtcgagcgat cagtcgcagg gctatcagcc gttcccggac gaggactatc
     2341 agccgagcat caccgaccgg catcgcggtt acgacgtcac gacgatcggc ggcaagtttt
     2401 cgcacgactt caccgacggg ttgcggctcg cgctcggcta tcaacgcatc gatgccgatc
     2461 tcgatcaaga tgctccggac acgtatctcg acgcgttcaa cgagcggcaa gaggacatct
     2521 tcacgggcac gctcgacttc gccgcgagcg acacggtgca gttctacgtg aagtcgtact
     2581 tccatcagtg ggactcctac tggacggagt acgacgtcga cctcgataac cccggcgacg
     2641 tcgtgctgat cgaccacgag gcgttttggg gcttcaagga ctacgggctc aatgcgctcg
     2701 cgaagctgtc gcccggcgat gccgtcgagt actacgtcgg ctacgactac cagaagtaca
     2761 gcggccgcga cgacgtgctg ttgatcgcgc cgaacaaaga gagcgtgcac gcggtgttcg
     2821 ggcaggtccg cacgacgccg gagctgttcg acaaagcccg catcgccttc ggtgtgcgcc
     2881 gcaatatgcc gagcgagagc gccgcggaga ccgtctggaa cgcgagcggc cagtacgact
     2941 tctcgcaacg cctgttcgcg cgcgccaccg tcggcacgtc gttccgcttg cccgatgcgt
     3001 acgagctgtt cgcggtcgac acgacgtgct gcttgggcaa tccgaacttg aagcccgaat
     3061 cgtccgagaa ctacaacgtc tcgctcggcg gcttgctcgg cgacaactcg atgatgaact
     3121 gggaagtcgt ctacttccgc cgctacgtga aggacctcat cgtcgacgtc gacttggacg
     3181 acggcagcgg gctcacgcaa gcgcagaacg tcgacaacga agtccgcgtg cgcggcgagc
     3241 aggtcgtgct gggcttccgg ccgagcgaca cgatctcggc gagcgtcagc tatacgcaca
     3301 acgactcgga ggcgctgaac agcaacacga cgtcgggcgg ctacacggag attcccggca
     3361 ttccgaagga ccagctcgcg gcctcgctga actacgagcc gacgggcaag ccgtttggcc
     3421 tgacgttgac gttgaacgac gtcggcagcg tgttcgacat cgtcggcggc gtcgggcccg
     3481 tggcgcgcgg cgactacacg gtcgtcgacg tcgcggcgcg cctgtacctc gacgacagcc
     3541 agcgccacaa ggtgatgttc cgcatcgaga acgcgttcga cgagacctac acgacgcgct
     3601 atcgacgctc gaccgtggac gggagcacgc cgcccgtgcg ctacgcggcc cgtaacctcg
     3661 gcatgccgcg cacgctgcac gtcagctacg tgcattcgtt ctagcgcgga acccgggacg
     3721 tgagcgccat cgcgccggtc aacgtgggag tgcggctcgg cgagcgcacg atggacgtgc
     3781 tcgtcaccgc acaccgttgg ctcggcactg cgctttgtct cgtgttcgcg acgtggttcg
     3841 cgtccggcat cgtcatgatg tatgcgggct acccgctgct cagcgagggc gacaagctcg
     3901 cgcgcacgcc gccgctcgac ctcgcggcgg cgcagctgac gccggccgag gcttaccggc
     3961 gcgcggggct cgactcgccg gtcgactacg tgcgcctcgg cacgatgctc gatcgcccga
     4021 cgtacttctt cctgaccggc ggcagctggg tcgcggtcgg cgccgatgac ggcaccgttc
     4081 ggccggccct cgacgccgac gccgcactcg gcgtcgcgcg cgcgttcgag gtcggcgagg
     4141 tcgcgccgcc gcgctatgcc ggcctgttcg agtcgccgga ccaatggacg atctatacga
     4201 gctgggcgcc gtacttgccg ggccacgatt tctcgccgtt tcacaagctc gagttcgacg
     4261 acggcacgga ggtgtccgtg ctcgccgcga ccggcgacgt catccagcgc acgacgccga
     4321 gcgagcgctt gtggggctac ctcggcgccg tcgtgcattg gatctatccg acgacgttcc
     4381 gtacgcgcca cgtcgacgcg tggaacgact tgatcgtctg gctgtcggcg ctcggcaccg
     4441 tgctctgcgt cagcggcgtc gccatcggca tttatttctt ccgctggcgg cgcgagcccg
     4501 gcgttgccgg ctcgccgtat cgcggctgga tgaagtggca tcactggata gggctcgtgt
     4561 tcggcgtact ggccgggccg tgggtgttca gcggcctgat gtcgatggac ccgtgggcgt
     4621 ggtcgacgac cgacgattcg ccggacggcg cgcaggtcga ccgcatgagc ggcgcgctcg
     4681 atctcgacgc gttcgttgcc gcgccggcca cggccgccgc ggcgtgccgc tcgcaggtca
     4741 acacgaagga gatacggctc gtgcagctcg ccggcgtgcc gtactactac tgctcacaag
     4801 cgccgaatcg ctccgcgctc gtcgccgcgg acggcagcgg cgctccgccg ttcgagagct
     4861 tcgcgcagag tgctctggtc gacgcggcgc gagccatcct gccgggtgcc gagatcgcat
     4921 cggccgagct gctcgatcat tacgacgcct attactaccc gggctgggaa gacaagctcg
     4981 agaacggcgc tgcaaagcgc ctgcccgtgc tgaagatcgc gttcgacgac gtcgagcgca
     5041 cgctcgtcta cgtcgatccg tacacggccg tgcccgtcaa ggtctacgac acgagcggcc
     5101 gttggtaccg ctggttgttc cacggcttgc acagcttgga tttcggcgcc ttgtatcggt
     5161 cgcgcccgct ctgggacttg ctcgtgctgc cgctgatgct cggcggcgtc gcagtctcgg
     5221 tgaccggagc ctggatcggc tacaagtggc tgaagcgcaa agttcgcaag ccaaaaccgc
     5281 agaagcgccg cacggcgcca gtggccccct gaggacgacg tgatgttgga agcgatcgga
     5341 gtcagcaaac ggttcggcga caagctcgcg ctcgacgacg tcacgctgcg tgtcgagccc
     5401 ggcgagattt actgcctgct cggcgcgaac ggcgccggca agacgacgct gatcaacgtg
     5461 ttcctgaact tcctcgcgcc gagcgagggc gaagcgcgca tcgacggcct cgacgtcggg
     5521 cggcacccgc ttcagacgaa gcagcgcgtc gcctacattc cggagcacgt gatgctgtac
     5581 ggagtgctct cggggctcga gaatctgcgt tatttcgctg cgctcggcac cggcaagaag
     5641 gcatcgcgcg gcgagttgct cgacctgctc gcgacggtcg ggctcg
//
LOCUS       DATLEE010000754         6388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7997118, whole genome shotgun sequence.
ACCESSION   DATLEE010000754 DATLEE010000000
VERSION     DATLEE010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7997118"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..256)
                     /locus_tag="VM692_16600"
     CDS             complement(80..256)
                     /locus_tag="VM692_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HUQ53851.1"
                     /translation="MTFLIALIFLGIVGSLGHALFSMASGPQASDRMVRALTIRIALS
                     VLLFGALAAGSYFG"
     gene            complement(253..444)
                     /locus_tag="VM692_16605"
     CDS             complement(253..444)
                     /locus_tag="VM692_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53852.1"
                     /translation="MKIAKPLLLLTTPIGVLGGLHEAWRLVGGLTFLIVVMLGILGFA
                     AASVVCIIRREQREQGGTS"
     gene            complement(573..2075)
                     /locus_tag="VM692_16610"
     CDS             complement(573..2075)
                     /locus_tag="VM692_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HUQ53853.1"
                     /translation="MGLARTLSRGQAGLDAYLVTVEVHIAGGLPGIAVTGLPAAAVRE
                     SKDRVRAALATSGHTLSASRITVHLGPADIPKTGGRFDLAIALGVVLAQDEALQWPVS
                     ATEFLGELALNGDLRPINGALPAALAAQHAGHALVLPEANAAEAALVPDAEVYAAAHF
                     KDVVRHLSGEQRLPRVAARSEPIPTGSAPDLADVRGQAFAKRALIVAAAGHHNLLMIG
                     PPGSGKSMLAERLSGLLPPLTRDEMLRVASIASVAGELRSAPPTSSPPFRAPHHTTSP
                     QALVGGGTRPRPGEISLAHRGVLFMDELPEFGRSALEALREPLESGVARISRVHDQLT
                     FPAEFMLVAAMNPCFCGYLGDGTERCKCTPYKLEQYRGRISGPLLDRFDLHVEVPRVP
                     FADIGEPAESSESAAVRETVVAARRVQLERAGKLNARLDARSLWRAVKLGAEQRALLK
                     RAADRWQLSARSSLRVLKVARTVADLAGSSAVTTEHVAEALQLRCRDRQV"
     gene            complement(2109..2375)
                     /locus_tag="VM692_16615"
     CDS             complement(2109..2375)
                     /locus_tag="VM692_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HUQ53854.1"
                     /translation="MDDKKSTRFSLDELARQLAESVPHNLKVLGQDLERNFKTLLQSG
                     IERMELVSREEFDVQRAVLERTRAKLEAMETRLADLEKALSSRP"
     gene            2572..3030
                     /locus_tag="VM692_16620"
     CDS             2572..3030
                     /locus_tag="VM692_16620"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HUQ53855.1"
                     /translation="MNYRLPSTTVLLALLGTTAFAADVYRSTAPDGTVSYSDRPQGSD
                     SQFVFSANTRPAAAPAAPAGNARAAANTTAQSAPQAPTDPTLPKGPSAATLREQRQKN
                     CDTARETAARYEVSRRLFRTNAAGEREYLDDAAVAAARAKAAADVQDWCG"
     gene            3008..4159
                     /locus_tag="VM692_16625"
     CDS             3008..4159
                     /locus_tag="VM692_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HUQ53856.1"
                     /translation="MSKTGAVESSVADRTPRTTLIPPPRPSAAADTAAAPKPGSELDS
                     FFIPDFCAPRMVLAVVLIAELVALTLALARPDAPFLTELARISMFLQWLGLTNAALLC
                     YSRSWLARLTVPQSSGAAFALILLNTVVISELALWLGTAFGAVGITERPTEHWPFLLR
                     NAGIAIIVTALLLRYFFVTHEWQRHVRAEAHSRIQALQARIRPHFLFNSMNTIAALTR
                     SDPKRAEEAVEDLADLFRATLRDSHSPLRLKEELELTRIYQRIEALRLGDRLAVIWDV
                     GALPMRAFVPGLTVQPLLENAIYHGIEPLEGGGKVTITGRVVDGEVELVVSNPVAPNP
                     EPGGQRNGNRLALDNIRQRLDLAYGGRGTLTVDQQPDRYRVTVRFPYSE"
     gene            4171..4911
                     /locus_tag="VM692_16630"
     CDS             4171..4911
                     /locus_tag="VM692_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HUQ53857.1"
                     /translation="MSVLIVDDELPARERLQRLVAELPGCAVAGVCSNGADALPLVHK
                     LKPAVVLLDIRMPGMTGIEVARHLGTLEHPPAIVFTTAYDEYALQAFESQAVGYLLKP
                     VRSERLAQALKQASRLSSPQLLGLATTQAEPLVARQHVAVRVRDELKLIPVKDIRYFR
                     ADQKYVTVRHSRGEDLIDEPLKQLEDEFARDFVRAHRSVLVAIAHVEALERAGGEESY
                     ALKLRGEAEPLAVSRRQLAELRKRLASH"
     gene            4926..5978
                     /locus_tag="VM692_16635"
     CDS             4926..5978
                     /locus_tag="VM692_16635"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HUQ53858.1"
                     /translation="MTDTLSKDSAAAPRGVQRAVLSSSVALLFAFVAIVIAGMLWWQY
                     RQFYVSLDETDTAAAAALERVRAEQRALQDGLADANDDVGTLRQLNTSLGERLDALPG
                     RFAALEERLDAVQGGSFDARGNLLRSEAEYYLTVANTELTLTADYESAVTALELADSR
                     LAEIANPELAPVREAIAGELLGLRSLRLPDIEGTVFGLGRLAARADELPLRADLPVNL
                     ASQSSDEREAEPGFGRLWIAIKGTLLDLVRIERRDDPVPQALSAAERALARRQLEVEL
                     ELARIAALRRDEQAFLSGLETAIAVLQRDFDSESADVEGALALLGELRSFDIDPEPPD
                     ISGSLNLLRAQRNEPR"
     gene            5978..>6388
                     /locus_tag="VM692_16640"
     CDS             5978..>6388
                     /locus_tag="VM692_16640"
                     /inference="COORDINATES: protein motif:HMM:NF018875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HUQ53859.1"
                     /translation="MRFSVAAVLAVVLGAFGAHFLLADRGYVLVNFRGYVIEMSVPGL
                     VIVLVTAYLAVRAVAAVVEAPRRWRAARSQKDFERRDSDLTAGLTQLIEGNWARSERL
                     LTQGLKHADAPLANYLLAAHAAQLQGAADRRDQWL"
BASE COUNT         1019 a         2210 c         2164 g          995 t
ORIGIN      
        1 aatcagtgcc gcgacgtgct ctgcgagctc cttgtagagc gtcatgccgg ggcgggcagg
       61 ccgccggccg cggcgccggt cagccgaagt agcttccggc cgcgagcgca ccgaacagca
      121 gcaccgacag cgcgatgcga atcgtcagcg cgcgcaccat acggtccgag gcctgcgggc
      181 ccgatgccat cgagaacaac gcatgcccga ggcttcctac gattccgagg aatatgagcg
      241 cgatgaggaa cgtcatgacg tgccaccttg ttctcgttgc tcacgacgaa tgatgcatac
      301 gacggacgcc gcggcgaagc cgagaatgcc gagcatcacg acgatcagga acgtgagccc
      361 gccgacgaga cgccaggctt cgtgcaggcc gccgagcacg ccgatcggag tcgtgagcag
      421 caatagcggt ttggcgatct tcatggacgc cactatcgag ccgatcgcgc atcgtgaata
      481 ggtgcagttg accagcaaaa aaagcgtgac agatggcctc gctatccggc tcgaacctaa
      541 cacgctgcgc gcgcaccacg ccagcgagag gctcaaacct gccggtcgcg gcagcgcagc
      601 tgcagtgcct cggcgacgtg ctctgtcgtc acggcggagc tgccggcaag atcggcaacg
      661 gtccgcgcga ctttcagcac gcgcaacgag ctgcgcgcgc tcaactgcca gcgatcggcc
      721 gcgcgtttca gcaacgctcg ctgctcggcg cccagcttca cggcgcgcca cagcgagcgc
      781 gcgtcgagcc gcgcgttgag cttgcctgcg cgctcgagct ggacgcgccg cgccgcgacg
      841 accgtctcgc gaacggccgc cgattcgctg ctctcggcgg gctcgccgat gtcggcgaac
      901 ggcacgcgcg gcacctcgac atgcaagtcg aagcgatcga gcagcgggcc cgagattcgc
      961 ccgcggtact gctcgagctt gtacggcgtg cacttgcagc gctccgtgcc gtcgccgaga
     1021 tagccgcaga agcaggggtt catcgcggcg acaagcatga actcggcggg aaaggtcagc
     1081 tggtcgtgca cgcgcgagat gcgcgctacg ccgctctcga gtggctcgcg cagtgcctcg
     1141 agcgcactgc gcccgaactc gggcagctcg tccatgaaca acacgccgcg atgcgcgagc
     1201 gagatctcgc cgggccgtgg tcgcgttccg ccgccgacga gcgcttgcgg cgaggtggtg
     1261 tgatgcggcg cgcggaacgg cggcgacgat gtcggcggcg cgctgcgcag ctcgccggcc
     1321 accgaggcga tgctcgcgac gcgcagcatt tcgtcgcgcg tcaacggcgg cagcaggccg
     1381 ctcaatcgct cggcgagcat gctcttgccg ctgccgggcg gcccgatcat caagaggttg
     1441 tggtgtccgg cggccgcgac gatgagcgcg cgtttcgcga acgcttggcc gcgcacgtcc
     1501 gcgagatccg gggccgagcc cgtagggatc ggctcgctgc gcgccgcgac gcgcggcaaa
     1561 cgttgctcgc cgctcaagtg ccgcacgaca tccttgaaat gggccgccgc gtagacttcg
     1621 gcgtcgggaa cgagggccgc ctcggccgcg ttcgcttcgg gcagcacgag cgcgtgaccg
     1681 gcgtgctgcg ccgcgagcgc ggcgggcaat gcgccgttga tcggccgcaa gtcgccattc
     1741 aacgcgagct cgccaagaaa ttccgtggcg ctaacgggcc actgcagcgc ctcgtcctgc
     1801 gcgaggacga caccgagcgc gatcgcgagg tcgaagcggc cgcccgtttt cgggatgtcg
     1861 gccggaccca agtgcacggt gatacggctc gcggagaggg tgtgccccga cgtcgcgagc
     1921 gcagcgcgta cgcggtcctt gctttcacgg accgccgcgg ccggcaaccc tgtgacggcg
     1981 atgccgggca gcccgccggc gatgtgcacc tcgaccgtga cgaggtaggc gtcgagcccg
     2041 gcctggccgc ggctcaacgt gcgggcgagc cccacacgcg gcctccaccc ggagcgcccg
     2101 cttgggcgct agggtcgtga cgacaacgct ttctcgagat ccgcgaggcg ggtctccatg
     2161 gcctcgagct tggcgcgcgt gcgctcgagg acggcacgtt gaacgtcgaa ctcctcacgg
     2221 ctaacgagct ccatgcgctc gatccccgac tgcagcagcg tcttgaagtt gcgctccagg
     2281 tcctgaccga gcaccttgag gttgtgcggt accgactcgg ctagctggcg cgcgagctcg
     2341 tccaggctga aacgcgtcga tttcttgtcg tccattgcac gatctccagg cgctgacggc
     2401 cagcgtcgaa actgaggttt tcggccagaa acgcgatgat agcccgcgct ctaggcgggg
     2461 ccgaagattt gacttaatgt tcggctcgaa agccgccgga atggcgtgaa accagtcaca
     2521 agaaccccag gcgagccttg ttagaatcgc gcgaatccgc cgaggccaga catgaattac
     2581 cgcctaccgt ccacgaccgt gttgctggcc ctgctcggca cgaccgcctt tgccgccgac
     2641 gtttaccgct cgaccgcgcc cgacgggacg gtcagctaca gcgatcgtcc gcaggggtcc
     2701 gactcgcagt tcgtcttttc ggccaatacg cgcccggccg ctgcgcccgc cgcgcccgcc
     2761 ggcaatgcgc gcgccgctgc aaacacgact gcgcagagcg cgccgcaagc gccgaccgat
     2821 ccgacgctgc caaagggtcc ttcggccgcc acgctgcgcg agcagcgcca gaagaattgc
     2881 gacaccgccc gcgaaacggc ggcgcgctat gaggtgtcgc gccggctgtt ccgcacgaac
     2941 gccgccggcg agcgcgagta cctcgacgac gcggcggttg ccgcggcccg cgcgaaggcg
     3001 gcggcggatg tccaagactg gtgcggttga gagctccgtc gccgaccgca cgccgcgcac
     3061 gacgttgata ccgccgccgc ggccgagcgc cgcggccgac acggcggcag ccccgaagcc
     3121 tggctcggag ctcgactcct tcttcattcc ggacttctgc gcgccgcgca tggtgctcgc
     3181 ggtcgtactg atcgcggagc tcgtcgcatt gacattggcg ctcgcgcgac ccgacgcgcc
     3241 gtttctgacc gagctcgcac gcatctcgat gttcctgcag tggctcggcc tcacgaatgc
     3301 ggcgctgctc tgctactcgc gcagctggct cgcgcggctc acggtgccgc aaagctcagg
     3361 cgccgccttc gcgctaattc tgctcaacac cgtcgtgatc tcggagctcg cgctgtggct
     3421 cggtacggcc ttcggcgccg tcggcatcac ggagcggccg accgagcatt ggccgtttct
     3481 gctgcgcaac gccggcatcg ccatcatcgt cacggcgctg ctgctgcggt acttcttcgt
     3541 gacgcacgaa tggcagcggc acgtgcgcgc cgaagcgcac tcgcgcattc aggcgctgca
     3601 ggcgcgcatc cgtccgcact ttttgttcaa cagcatgaac acgatcgcgg cgctgacgcg
     3661 cagcgatccc aagcgcgccg aagaggccgt cgaggatctc gccgatctgt tccgtgccac
     3721 gctgcgcgat tcccatagcc cgttgcgcct caaagaagag ctcgagctca cgcgcatcta
     3781 ccagcgcatc gaggcgctgc ggctaggcga tcgtctggcc gtgatctggg acgtcggcgc
     3841 gctgccgatg cgcgcgttcg tgccgggcct cacggtgcag ccgctgctcg aaaacgcgat
     3901 ctatcacggc atcgagccgc tcgaaggcgg cggtaaagtc acgatcacgg gccgcgtcgt
     3961 cgacggcgag gtcgagctcg tggtcagcaa cccggttgcc ccgaatcccg agcccggcgg
     4021 gcagcgcaac ggcaaccgcc tcgcgctcga caacatccgc caacgcctcg atctcgccta
     4081 cggcggccgc ggcacgctga cggtcgacca gcagccggat cgataccgcg ttaccgtgcg
     4141 ctttccgtat tccgaatgaa cgccgtcccg ctgagcgtct tgatcgtcga tgacgagctg
     4201 ccggcacgcg agcgcctgca gcggctcgtc gcggagctac cgggctgcgc cgtcgcgggc
     4261 gtctgctcga atggcgccga cgccttgccg ctcgtccata agctgaagcc cgccgtcgtc
     4321 ttgctcgata tccgtatgcc cggcatgacg ggcatcgagg ttgcgcgtca cttgggcacg
     4381 ctcgagcacc cgccggcgat cgtcttcacg acggcgtatg acgagtacgc gctgcaggca
     4441 ttcgaatcgc aggccgtcgg ctatctgttg aaacccgtcc gcagcgagcg tctcgcgcaa
     4501 gcgttgaagc aagcctcgcg gttgagctct ccgcaactgc tgggtctcgc gacgacacaa
     4561 gccgagccgc tggtcgcgcg gcagcacgtc gcggttcgcg tccgcgacga gctgaagctg
     4621 atacccgtga aagacatccg ctacttccgc gccgatcaga aatacgtcac ggtccggcat
     4681 agtcgcggcg aggatctcat cgacgagccg ttgaagcagc tcgaggacga gttcgcgcgc
     4741 gacttcgtgc gtgcgcaccg cagcgttctc gtcgcgatcg ctcacgtcga ggcgctcgag
     4801 cgcgccggcg gcgaagaaag ctacgcgctc aagttgcggg gcgaggcaga gccgctcgcc
     4861 gtgagccgcc ggcagctcgc ggagctgcgc aaacgcttgg ccagtcactg aggaacgcgc
     4921 gcgccatgac cgacacgctt tcgaaagact ccgcggcggc gccgcgcggt gtgcaacgcg
     4981 ccgtcttatc gagctcggtg gcgctgctgt tcgcattcgt cgcgatcgtg atcgcgggca
     5041 tgttgtggtg gcagtaccgg caattctatg tctcgctcga cgagaccgac accgcggcgg
     5101 ctgcggcgct cgaacgcgtg cgcgccgagc agcgtgcgct gcaagacggg ctcgccgacg
     5161 cgaacgacga tgtcggcacg ttgcgccagc tcaacacgag cctcggcgag cggctcgacg
     5221 ctttgccggg ccggttcgcg gcgctcgagg agcggctcga tgcggtgcag ggcggctcgt
     5281 tcgacgcgcg cggcaatttg ctgcgctcgg aggccgagta ctacctgacg gtcgccaaca
     5341 cggagctcac gttgacggcg gattacgaaa gcgccgtgac ggcgctcgag ctcgccgata
     5401 gccggctcgc cgagattgct aaccccgagc tcgcgccggt tcgcgaggcg atagcgggcg
     5461 agctgctcgg gctgcgcagc ctgcgtttac ccgacatcga ggggaccgtc ttcggcctcg
     5521 gccggctcgc cgcgcgcgcc gacgagttgc cgctgcgcgc cgacttgccg gtgaacctcg
     5581 cgagccaaag cagcgacgag cgcgaggccg agcccggctt cggccggctg tggatcgcga
     5641 tcaagggcac gctgctcgat ctcgttcgca tcgagcggcg cgacgacccc gtgccgcagg
     5701 cgctctcggc tgccgagcgc gcgctcgcac gccgccagct cgaagtcgag ctcgagctcg
     5761 cgcgcatcgc agcgctgcgc cgcgacgagc aagcgttctt gagcgggctc gagacggcga
     5821 tcgcggttct gcaacgtgac ttcgatagcg agtccgccga cgtcgaaggc gcgctcgcgc
     5881 tgctcggcga gctgcgtagc ttcgacatcg atcccgagcc gccggacatc agcggctcgc
     5941 tgaatctatt gcgcgcgcag cgcaacgagc cgcgctgatg cggttcagcg tcgcagccgt
     6001 gctcgccgta gtgctcggcg cattcggcgc gcactttttg ctggcggatc gcggctacgt
     6061 gctcgtgaac ttccgcggct acgtcatcga gatgtcggtg ccggggctcg tcatcgtgct
     6121 ggtgacggcg taccttgccg tccgcgctgt cgcggccgtc gtcgaagcgc cgcggcgctg
     6181 gcgtgccgcg cgctcgcaga aggacttcga gcgccgcgac agcgatctga cggcaggtct
     6241 cacgcagctc atcgaaggca actgggcgcg cagcgagcgg ctgctgactc aggggctcaa
     6301 gcacgcagac gcaccgctcg cgaactatct gctcgcggcg cacgccgcac agctgcaagg
     6361 tgctgcggat cggcgcgatc agtggctc
//
LOCUS       DATLEE010000755         4657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8938216, whole genome shotgun sequence.
ACCESSION   DATLEE010000755 DATLEE010000000
VERSION     DATLEE010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8938216"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="VM692_16645"
     CDS             <1..1495
                     /locus_tag="VM692_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HUQ53860.1"
                     /translation="IASAGDALHDPVTRYLVGGALTTDQTHYEYVVPAYRDRWQLALS
                     QFTGARVRLESASRGFAKVVVRVDGPQVGLAYALLDLEASEQNPLGPVYARVTERHAT
                     RAISYRAADGLEISGYLTVPSDKAARDLPLVVLVHGGPAARDTGDFDWWPQALASQGY
                     AVLQANFRGSLLDTKFMLAGFGEWGRKMQTDLSDGVRHLADQGTIDPARVCIMGASYG
                     GYAALAGVTLDAGVYRCAVSVAGVGDIEAMLEWQQANTEEREQRFWNKFMGIAGPGDP
                     AIHAISPAEHATAVTVPVLLVHGKDDTVVPYEQSEAMLRALRRAGKEVELVTLRREDH
                     WLSRRDSHADAPSVDRVLAPAQSARLASTRPALARSSTCKRTRRHVEVFSTHVVEFVA
                     SREGRGRTLRRVAQPFSRRPRCVNPLSRSVFSAPSRSSPARQSSLPRPTDGSSRNLSR
                     CAIPTSISAKRPEWQACTRGFAMPRSKFATPMQRHERCSSRRVNVPA"
     gene            1693..2571
                     /locus_tag="VM692_16650"
     CDS             1693..2571
                     /locus_tag="VM692_16650"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HUQ53861.1"
                     /translation="MRVFVCVAEHLNFTRAGEALGVTPGAASLQIRALEDYLSRQLFR
                     RNGRVVELTTEGAELLPRVQQALGDIERALAATRGDRSGGPLRVTMLASFLQSWLLPR
                     LPRLRADHPRIDLQVHTSEAMVDLVRDDQHAAIRLGTGDWPGVTAEKLLEEWLVPVCT
                     PELYSRYGPLQRRQDIARYPLVHSTSEPWTAWLLENKAMEDTTSPFTGTRVDDSAAIV
                     RLAQQGLGLALARWSLAGDDVAAGRLVAASDKPLSFFRTYWLVQSKHGRSHPALAQFR
                     EWLKSEIAKFPRPPGI"
     gene            complement(2585..3208)
                     /locus_tag="VM692_16655"
     CDS             complement(2585..3208)
                     /locus_tag="VM692_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUQ53862.1"
                     /translation="MIAVVTVHGLWMRGASMGVLRGRLAPHGFAFHAFDYPSVKGSLR
                     ANVAALAAFVDSVPGDTVHFVGHSLGGVLICALLEDRLPARLGRVVCLGAPLRGSRSA
                     ARLARWPGGHRLVGRCLADVQARGGFSTWPADVEVGSIAGRMPLGAGRLLGRFRGPND
                     GTVAVAETQIAGLTDHVVLPVTHFALLWSSAVAAQTEHFLLHARFRR"
     gene            complement(3205..3654)
                     /gene="nudB"
                     /locus_tag="VM692_16660"
     CDS             complement(3205..3654)
                     /gene="nudB"
                     /locus_tag="VM692_16660"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HUQ53863.1"
                     /translation="MAAVAEFKRPESVLIVVHTPALDCLLLERVEPRGFWQSVTGSLR
                     PAETPADCAARELQEETGLEPQGLRDAQIQRSFPILPAWRSRYAPGVDSNVEHQWYLE
                     VPEVRAVRIEPTEHVAYLWLPIDAAIERVASWTNREALQLLKDSRRA"
     gene            complement(3662..>4657)
                     /gene="aspS"
                     /locus_tag="VM692_16665"
     CDS             complement(3662..>4657)
                     /gene="aspS"
                     /locus_tag="VM692_16665"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HUQ53864.1"
                     /translation="VLQVELPKPFPRMTYADALRRYGSDKPDLRNPLELVEVADLFKG
                     VEFKVFAGPAADPKSRIAALKLPKGGELTRKEIDDYTTLVARHGAKGLAYIKVNERTK
                     GRDGLQSPILKFLPDAAIEGVLERTKAVDGDLVFFGADKSGVVNESLNALRGALGRDR
                     KLLQGEWAPLWVVDFPLLEALPGGGWTPLHHPFTSPRDASAAALRAAPGDAIARAYDI
                     VLNGYEVGGGSVRIHDPELQSTMFDLLGIGKEEAELKFGFLLTALDFGAPPHGGIAFG
                     VDRLVMLMAGTDNIREVIAFPKTATASDLLTGAPSPADEKQLKELGIRLKSAAPG"
BASE COUNT          791 a         1598 c         1552 g          716 t
ORIGIN      
        1 aatcgccagt gccggcgatg cgctgcacga tccggtgacg cgctacctcg ttggcggcgc
       61 tctcacgacc gatcagacgc attacgagta cgtggtcccc gcgtaccgcg atcgctggca
      121 gttggcactc agccaattca ccggcgcgcg agtgcggctc gagtcggcct cccgcggttt
      181 cgcgaaagtc gtcgtgcgcg tggatggacc gcaggtcggc ttggcttatg cattgctcga
      241 tttggaagcc agcgagcaaa accctctcgg cccggtgtat gcgcgcgtca cagaacggca
      301 cgcgacacga gcgatcagct accgcgccgc cgacggcctc gaaatttcag gctatctgac
      361 cgtcccgagc gacaaagcgg cgcgagacct cccgctcgtc gtgctggtgc acggcggccc
      421 cgcagcgcgc gacaccggcg acttcgattg gtggccgcag gcgctggcgt cgcagggcta
      481 cgccgtgctg caggcaaact tccgcggctc tctgctagac accaagttca tgctggcggg
      541 attcggggag tggggtcgca agatgcagac cgatctctcg gacggggtgc gccatctcgc
      601 ggaccaagga acgatcgatc ctgcccgtgt ctgcatcatg ggcgccagct atggcggtta
      661 tgcggcgctt gccggagtga cactcgacgc cggtgtgtac cgctgcgctg tgtccgtggc
      721 gggcgtcggc gacatcgagg ccatgctcga gtggcagcag gccaataccg aggaacgaga
      781 gcaacgcttc tggaacaaat tcatgggcat cgccggaccc ggcgatcccg caatccacgc
      841 gatctcgccg gctgagcacg ccacggccgt caccgtgccg gttctgctag tgcacggcaa
      901 ggacgacacc gtcgtgccgt acgagcagag cgaagcaatg cttcgagcgc taaggcgtgc
      961 cggcaaggaa gtcgagctcg tcacactgcg gcgggaagat cactggctct cgcggcgaga
     1021 ctcgcacgca gatgctccaa gcgtcgaccg agttcttgcg ccggcacaat ccgcccgact
     1081 agcgagcacg cgcccggcac tggctcgctc gagcacctgc aaaagaacgc ggcgtcacgt
     1141 tgaggttttc tcaacgcacg ttgtcgagtt tgtcgcatcg agagaaggcc gcggccgcac
     1201 actgcgccgc gtcgcccaac cgttttccag gagacctcga tgcgtaaatc ccctttctcg
     1261 ctcggtgttc tcggcaccct cgcgatcctc tccggctcga cagtcctccc tgccgcggcc
     1321 gacggacggt tcgtcgagga atctctcgag gtgcgctata ccgacctcga tctcggcaaa
     1381 gaggccggag tggcaagctt gtacgcgcgg cttcgcaatg ccgcggagca agtttgcgac
     1441 gccgatgcag cgccacgagc gctgttcctc tcgtcgagtc aacgtgcctg cgtgacggcc
     1501 gcactggaac aagccgtcgc caatgtagat cgccccgcgc tcacggctta ccatgccgcg
     1561 agagcgagcc aggcgggcgt acgcggcccg cggctcgccg ccgcgaggga ctgacgtatc
     1621 gcaacgagaa cgcgcaagcg gcgcacccaa gccgccgttc cgtccgcacc gcgcagactg
     1681 ccgctcggca gcctgcgcgt tttcgtttgc gtcgccgagc atctgaactt cactcgcgcc
     1741 ggcgaggcgc tcggcgtcac gccgggcgcc gcgagcttgc agatccgggc gctcgaagac
     1801 tatctgagcc ggcaactgtt tcggcgtaac ggccgcgtcg tcgagctgac gacggaaggc
     1861 gcggagttgc taccgcgcgt ccagcaagcc ctcggcgaca tcgagcgcgc gctcgcggcg
     1921 acacgcggcg atcgcagcgg cgggccgctg cgcgtgacga tgctggcgtc gtttctgcaa
     1981 agctggctgc tgccgcgctt gccgcggcta cgcgccgacc acccgcgtat cgacctgcag
     2041 gtgcatacct ccgaggccat ggtcgatctc gtgcgcgacg accagcacgc ggcaatccgg
     2101 ctcggcacag gcgactggcc gggcgtcact gccgagaagc tgctcgaaga atggctcgtg
     2161 cccgtctgca cgcccgagct ctactcgagg tatgggccgc tgcaacggcg ccaggacatc
     2221 gcccgctatc cgctcgtgca tagcacgagc gagccgtgga cggcgtggct gctcgagaac
     2281 aaagccatgg aggacacgac gtcgccgttc acgggcacgc gcgtcgacga ttcggccgcg
     2341 atcgtgcggc tcgcgcagca aggacttggg ctcgcgctcg cgcgttggag cctggccggc
     2401 gacgacgtcg ccgcgggccg gctcgtcgcg gcgagcgaca aaccgttgtc gttcttccgt
     2461 acctactggc tcgtgcaatc gaagcatggc agatcgcacc cggcgctcgc gcagttccgc
     2521 gagtggctca agagcgagat cgccaaattt ccgcggccgc ccgggatctg aaaccgaagc
     2581 aggctcagcg ccggaatcgc gcgtgcagca ggaagtgctc cgtctgtgcc gcgacggcgg
     2641 aggaccacag cagcgcgaag tgtgtgacgg gcagaacgac gtggtccgtc aaaccggcta
     2701 tttgcgtttc ggctacggct accgtgccgt cgttcggccc gcggaaacgc ccgagcagcc
     2761 ggccggcgcc gagcggcatg cgcccggcga tactgccgac ctcgacgtcg gccggccaag
     2821 tcgagaagcc gccgcgcgcc tgcacgtcgg cgagacacct accgaccaag cggtggccgc
     2881 cgggccagcg cgcgagccgc gccgcgctgc ggctccctct caacggcgcg ccgaggcaaa
     2941 caacacgacc gagtcgggcg ggcaaccggt cctcgagcaa cgcgcaaatc agcacaccgc
     3001 ccaagctgtg accgacgaag tgcacggtgt cgccgggaac cgagtcgacg aacgcggcga
     3061 gcgcggcgac gttcgcgcgc agtgagccct tgaccgacgg gtagtcgaac gcatggaacg
     3121 cgaacccgtg cggcgcgagc cgcccgcgca gcacgcccat cgacgcgccg cgcatccaca
     3181 agccgtgcac ggtgacgacc gcaatcacgc gcggcgcgaa tccttgagga gctgcaacgc
     3241 ctcacgattc gtccacgacg cgactcgctc gatggccgcg tcgatcggca gccacagata
     3301 cgcgacgtgc tcggtgggct cgatacgcac ggcgcgcact tcggggacct cgagatacca
     3361 ctggtgctcg acgttcgagt cgacgccggg agcgtagcgt gagcgccatg ccggcaggat
     3421 cggaaacgag cgctggatct gcgcgtcacg caaaccctgc ggctcgagac ccgtctcttc
     3481 ctggagctcg cgcgcggcac agtcggccgg cgtctcggcg gggcgcagcg agcctgtcac
     3541 ggactgccaa aagccgcgcg gctcgacgcg ctcgagcagc aggcaatcga gcgccggcgt
     3601 gtgcacgacg atcagcacgg actccggtcg cttgaactct gcgacggcgg ccatgcgctc
     3661 gtcagcccgg agccgcgctc ttcaatcgga tgccgagctc cttgagctgc ttctcgtcgg
     3721 cagggctcgg cgctcccgtc agcaaatcgc tggccgtagc ggtcttcggg aacgcgatga
     3781 cctcgcggat gttgtcggtg ccggccatca gcatcacgag gcggtcgacg ccgaatgcga
     3841 tgccgccgtg cggcggcgcg ccgaaatcga gcgccgtcaa caggaagccg aacttgagct
     3901 ccgcctcttc cttaccgatg ccgagcaagt cgaacatcgt cgattgcagc tcgggatcgt
     3961 gaatacgaac ggagccgccg ccgacctcgt agccgttcag cacgatgtcg tacgcgcgcg
     4021 cgatcgcgtc gccgggcgcc gctcgcaacg cggccgccga tgcgtcgcgg ggcgacgtga
     4081 acggatgatg caacggcgtc cagccgccgc cgggcagtgc ctcgagcagc gggaaatcga
     4141 cgacccagag cggcgcccac tcgccttgca ggagcttgcg atcgcgcccc aaggcgccgc
     4201 gcagcgcatt cagcgattcg ttgacgacgc cgctcttgtc ggcgccgaag aacacgaggt
     4261 cgccgtcgac ggcttttgtg cgctcgagca cgccttcgat cgccgcatcc ggcaggaact
     4321 tcaagatcgg cgactgcaag ccgtcgcggc ctttcgtgcg ctcgttgacc ttgatgtagg
     4381 cgagaccctt cgcgccgtgc cgagcgacga gcgtcgtgta gtcgtcgatc tccttgcgag
     4441 tgagctcgcc gcctttcggc agcttcagcg ccgcgatgcg gctcttcgga tccgccgcag
     4501 ggcccgcgaa gaccttgaac tcgacgcctt tgaaaagatc ggcgacctcg acgagctcga
     4561 gcgggttacg caagtcgggc ttgtcgctgc cgtagcggcg cagcgcgtcg gcgtacgtca
     4621 tgcgcgggaa cggcttcggc agctcgactt gcagcac
//
LOCUS       DATLEE010000756         3138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_11168068, whole genome shotgun sequence.
ACCESSION   DATLEE010000756 DATLEE010000000
VERSION     DATLEE010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11168068"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VM692_16670"
     CDS             <1..599
                     /locus_tag="VM692_16670"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HUQ53865.1"
                     /translation="PIVALTAHDAVRYREKCLAADIDDILSKPYTLDDCRRMLRQWLA
                     PVEGTAAVPTLTAPAPDSATPSAPELLAVVDLNAVAALRQLGVPLRGLPRGAGKQADL
                     YSKLVELFRTSSTQSLTDLTNALEQDDLKAVAAVCHKLAAAAANVGALAYAAKVRETE
                     RQALAGERTTTCNLSAALCEAHLPLLDALQQQRLRATA"
     gene            596..2356
                     /locus_tag="VM692_16675"
     CDS             596..2356
                     /locus_tag="VM692_16675"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ53866.1"
                     /translation="MSEENTKKRVAIIADDEDLGRVLLGEAVGAVGLEVLSFADGGTA
                     LDAALENDVAVVLLDVDMPVLDGNEVCRRLRAEPRFSSVPIVIVTGHQDSDAIRRAFE
                     AGATYFISKPVNWALLPHRLEYILRNAAAAEHIERLAYYDTLTGLPNRRRCVDLLGRM
                     VAAAKGAGEAVAFIYLDLNNFKRVNDTFGHAVGDQVLRTVAGRLTSTLASFADKHPLV
                     ARFGGDEFVVAVRDAQARDVGLQLAAACSDAFLKPIVYDSLEFYSAPSVGMAVFPDDG
                     ADVATVLKHADTAMYQAKSGAADPIAVYAPAMSNHLEHWLELEAKLRRAIESDVLTLM
                     FQPKFSLANNRLVGVEALVRWCDDEYGDIPPSRFVQIAEDSGLIIEMSAWVVRAACRQ
                     MCKWLDRGLWIPVAINCSGKELLHGDPARVIETEAAAARVPTSLIEVEITETLLVKDS
                     SAVKNVLQRIRQLGCHIALDDFGTGYSSLAYITRFPPDRIKIDRAFVRDVDRSESDAA
                     VANAILSLAKSLGVTVTAEGIERPGQLEWLRARGCNEAQGFLLSEPLTPAELERRFLS
                     KGAVPPPEESARDARRSQSA"
     gene            complement(2370..>3138)
                     /locus_tag="VM692_16680"
     CDS             complement(2370..>3138)
                     /locus_tag="VM692_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HUQ53867.1"
                     /translation="AGAHIAELGFAPDARAAIKQAADVNGERGSFDWLHVNSATYVGP
                     NRWFDAGDQRFAPNNVIISSRQASLLAIVARDGKIVWQLGPDFSTTEQLRKIRQIIGQ
                     HHAHFIPKGLPGAGNLLVFDNGGSSGYGFAGPIAPNGVGAFARATSRVLEINPVTLEL
                     VWSYAAPHFFSTNISSAQRLPNGDTLITEGAGGRVFEVTNDATIVWEYLNPLFAGPRA
                     SNDVYRAYRLPYDWIPQLTKPAEKAVTPPALGEFALP"
BASE COUNT          514 a         1055 c         1053 g          516 t
ORIGIN      
        1 tgccgatcgt cgcgctgacg gcgcacgatg ccgtgcgcta tcgcgaaaaa tgcctcgcgg
       61 cggacatcga cgacatcttg agcaagccgt acacgctcga cgattgccgc cggatgctgc
      121 gccagtggct cgctcccgtc gaaggcacgg cggctgtgcc gaccctaacc gcgcccgccc
      181 ccgacagcgc gacgccttcc gcgcccgagc tgctcgccgt cgttgacttg aatgccgtcg
      241 cggctctgcg ccagctcggc gttccccttc ggggactacc aaggggcgcc gggaagcaag
      301 ccgatctcta ttcaaagctc gtcgagctgt tccgcacgag ctcgacgcag tcgctcaccg
      361 acctcacgaa cgcgctcgag caagacgacc tcaaggcggt tgccgcggtt tgccacaagc
      421 tcgccgcggc ggccgccaac gtcggcgcgc tggcctacgc cgccaaggtc cgggagaccg
      481 agcgccaagc gctggccggt gaacgcacga cgacctgcaa tctcagcgcg gcactgtgcg
      541 aagcgcactt accgctactc gacgcccttc agcaacaacg tttacgagcc acggcatgag
      601 tgaagagaac accaagaaac gagtcgccat cattgccgac gacgaggacc tgggccgcgt
      661 cctgctcggc gaggcggtcg gggccgtcgg tctcgaagtg ctgtcgttcg ccgacggcgg
      721 caccgcgctc gacgcggcgc tcgagaacga cgtggccgtc gtgctgctcg acgtcgacat
      781 gcccgtgctc gacggcaacg aagtttgccg gcgcctgcgc gccgagccgc gcttcagcag
      841 cgtgccgatc gtcatcgtca cggggcatca ggattccgac gccatacgcc gggccttcga
      901 agctggcgcc acgtacttca tctcgaagcc cgtcaactgg gcgctgctgc cgcaccggct
      961 cgagtacatc ttgcgcaacg ccgcggcggc tgagcacatc gagcggctcg cgtactacga
     1021 cacgttgacg ggcctgccga atcgccgccg ctgtgtcgat ctcttggggc gcatggtcgc
     1081 ggctgccaaa ggagccggcg aggccgtcgc cttcatctat ctagacttga acaacttcaa
     1141 gcgcgtcaac gacaccttcg gccatgccgt cggcgatcag gtgctgcgca ccgtcgcggg
     1201 ccggttgacc agcacgttgg cgagctttgc ggacaagcat ccactcgtgg cccgcttcgg
     1261 cggggatgag ttcgtcgtcg ccgtgcgcga cgcgcaggcg cgcgacgtcg gcctgcagct
     1321 cgcagccgcg tgcagcgacg cgttcttgaa gccgatcgtt tacgactcgc tcgagttcta
     1381 ctcggcaccg agcgtgggca tggccgtgtt ccccgacgac ggcgcggacg tcgcgacggt
     1441 cctcaagcac gccgacacgg cgatgtacca ggccaagtcc ggcgcggcgg acccgatcgc
     1501 cgtctacgct cccgcaatga gcaatcacct cgagcactgg ctcgagctcg aggccaagct
     1561 gcggcgcgcg atcgaaagcg acgtgctgac actgatgttc caaccgaagt tcagcctcgc
     1621 gaacaatcgg ctcgtcgggg tcgaagcgct cgtgcgctgg tgcgacgacg agtacggcga
     1681 tattccgccc tcgcgcttcg tacaaatcgc cgaggacagc ggcctcatca tcgagatgag
     1741 cgcctgggtc gtgcgtgcgg cgtgccggca gatgtgcaag tggctcgacc gcggtttgtg
     1801 gatccccgtc gcaatcaact gctccggcaa ggagctcctg cacggcgacc ccgctcgcgt
     1861 gatcgaaacg gaagcggcgg ccgcgcgcgt gccgacttcg ttgatcgagg tcgagatcac
     1921 cgagacgttg ctcgtgaagg attcgagcgc ggtgaagaac gtgctgcagc gcattcgcca
     1981 gctcggctgc catatcgcgc tcgacgactt cggcaccggc tattcgtcgt tggcctacat
     2041 cacgcgtttt ccgccggacc gcatcaagat cgacagggcc ttcgtgcgcg acgtcgaccg
     2101 ctcggagagc gacgccgccg tcgcgaacgc gattttgtcg ttggccaaga gcctcggagt
     2161 caccgtcacg gccgagggca tcgaacggcc cggtcagctc gaatggctgc gggcgcgcgg
     2221 ctgcaacgag gcgcaggggt tcctgctctc cgagccgctg acaccggcgg agctcgagcg
     2281 gcgcttcctg tcgaaaggcg ccgttcctcc gcccgaagaa tccgcacgcg acgcgcgccg
     2341 ctcgcagtcc gcgtagcgcc gccgaagcgc tatggcaagg cgaactcgcc gagcgcgggc
     2401 ggcgtgacgg ctttctcggc cggcttggtg agctgcggga tccagtcgta cggcaggcga
     2461 taggcgcgat agacgtcgtt cgacgcacgc gggccggcga acagtgggtt caagtattcc
     2521 cagacgattg tggcgtcgtt cgtgacctcg aacacgcggc cgccggcgcc ttcggtgatc
     2581 agcgtgtcgc cgttcggtag gcgctgcgcg ctgctgatgt tggtgctgaa gaagtgcggg
     2641 gcggcgtacg accagacgag ctcgagcgtc acgggattga tctcgagcac gcgcgacgtg
     2701 gcgcgcgcga acgcgccgac gccgttggga gcgatcgggc ccgcgaagcc gtagccgctc
     2761 gagccgccgt tgtcgaagac gagtaagttg cccgcgcccg gcagaccctt cgggatgaag
     2821 tgcgcgtggt gctggccgat gatctgccgg atcttgcgca gctgctccgt cgtactgaaa
     2881 tcaggcccga gctgccagac gatcttgccg tcgcgggcga cgatcgcgag caggcttgct
     2941 tggcggctgc tgatgatgac gttgttcggc gcgaagcgct gatcgccggc gtcgaaccag
     3001 cgattcggcc cgacgtaggt cgcagagttc acgtgtagcc agtcgaacga cccgcgttcg
     3061 ccgttgacgt cggccgcttg cttgatcgct gcgcgcgcat cgggcgcgaa gccgagctcg
     3121 gcgatgtgcg cgccggcc
//
LOCUS       DATLEE010000757         3459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_7448520, whole genome shotgun sequence.
ACCESSION   DATLEE010000757 DATLEE010000000
VERSION     DATLEE010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7448520"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VM692_16685"
     CDS             complement(<1..507)
                     /locus_tag="VM692_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUQ53868.1"
                     /translation="MNLTQRIACAAAGVAAAVGAFAQPSAPSDRAAVDAEVLRHFQAL
                     VRMDTQSPPGNEKPAADYVRQVLEREGIPVQTFALEANRPNVVARLTGNGRKRPLLIM
                     GHTDVVNVDPKKWQHPPFSADREGGHIYGRGTVDDKDNLTAALMTMLLLKRNLVPLDR
                     DVILLAEAG"
     gene            complement(518..982)
                     /locus_tag="VM692_16690"
     CDS             complement(518..982)
                     /locus_tag="VM692_16690"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ53869.1"
                     /translation="MNSAIEDLVSGFERGTLSRRQLVQGLTAIAAAGAAAPAAAQTTP
                     FRSTRIDHISIQVTDMARSVEFYEKIFGLKILSEDKENEIVRMGVTKIIVSLHHKQPV
                     GIVDHFAIAIDDFNREAVTAELAKHGLKAEQNLDYGFYVRDPEGMPVQIVGT"
     gene            complement(1067..3091)
                     /locus_tag="VM692_16695"
     CDS             complement(1067..3091)
                     /locus_tag="VM692_16695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUQ53870.1"
                     /translation="MKRVLKRFDERALKLVLAAFFLALALPAGVLIVQAYGQLKWQAF
                     RSTQVAAEELAARVDATLRAAIGTEDARSFGAYSFLVVEGDAAANFVQRSPLSAFPVE
                     SAIPGVLGYFQVDAAGKLTTPLLPEAGVAASNYGIAPGEEAARAERVAQVRDLLVRNQ
                     LVRGPRDEPAPAPAEVERERSQRRLNASSPASASASFVQEGFDRLSVDAPPLRRAVGA
                     RDDDAGAAREVVAPAAPSVAPPAAQLVTVVATFAEQRQKRLEQSLVAEPAAEQDAAEL
                     GAVADAKAEVSVRMFASEVDPFELGVLDDEHLVLFRNVWRDGQRYVQGALVERAAFVM
                     GVIDSPYRASNVGSVARLNVDLQGRPWEVFGPLISSEENARSTELTGTVLHRARLSPP
                     FGDLELAFVVDRLPRAPGTALLAWVAVTLGAVLVGGFIFMYRFGVGQMRLARQQQDFV
                     SAVSHELKTPLTSIRMYGEMLKAGWADDAKRQTYYDYIHSESERLSRLIENVLQLARL
                     TRNTQRFDLRSVTVAELLDLVRSKVATQVERAGFTLTLRNETPEGTAVVADADCFAQI
                     FINLVDNALKFSAGATTKTVEIAARRGGDGKVLLTVRDFGPGVPKTQMKKIFELFYRA
                     ENELTRETVGTGIGLALVRQLAAAMDARVDVRNCEPGAEFRLQFPPAATG"
     gene            complement(3088..>3459)
                     /locus_tag="VM692_16700"
     CDS             complement(3088..>3459)
                     /locus_tag="VM692_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUQ53871.1"
                     /translation="LVLRVKAVLRRSRTGAASQAEIKLAGDVAIDTRNLSGRRGSEPL
                     TFTRREIEILAYLQRNTERPVSRDELLTRVWGYDRSAEIETRTVDIHIAKLRRKIEVD
                     AKEPRNLITVRGTGYRLVVSA"
BASE COUNT          565 a         1171 c         1172 g          551 t
ORIGIN      
        1 gccggcctcg gcgagcaaga tcacgtcgcg atcgagcggc acgagattgc gcttcagcag
       61 cagcatcgtc atgagcgccg ccgtcagatt gtccttgtcg tcgacggtgc cgcggccgta
      121 gatgtggccg ccctcgcggt cggcgctgaa cggcggatgc tgccacttct taggatcgac
      181 gttgaccacg tccgtgtgcc ccatgatcag tagcggccgt ttgcggccgt tgcccgtgag
      241 gcgtgcgacg acgttcggcc ggtttgcctc gagcgcgaac gtctgcacgg ggatgccttc
      301 gcgctcgagc acctgcctga cgtagtcggc ggcgggtttc tcgttgccag gcgggctctg
      361 cgtatccatg cgcacgagcg cctgaaaatg ccgcagcacc tcggcgtcga cggcggcacg
      421 atcggacggc gcactcggct gcgcaaaagc gccgaccgca gctgcgaccc cggccgcggc
      481 gcaggcgata cgctgagtga ggttcatcga gcgctcctca agtgccgacg atctgcacgg
      541 gcatgccttc gggatcgcgc acgtaaaaac cgtagtcgag attctgctcg gccttgaggc
      601 cgtgcttggc caactcggcc gtcacggcct cgcggttgaa atcgtcgatc gcgatcgcga
      661 agtggtcgac gatgccgacg ggctgcttgt gatgcagcga cacgatgatc ttcgtgacgc
      721 ccatgcgcac gatctcgttc tctttgtctt cgctcaggat cttgagcccg aagatcttct
      781 cgtagaactc caccgagcgg gccatgtcgg tcacttggat gctgatgtga tcgatgcgcg
      841 tcgagcggaa cggcgtcgtt tgcgccgcgg ccggcgccgc ggcgcccgcg gcagcaatgg
      901 ccgtcaagcc ttgcacgagc tgacggcgcg acaaagtgcc gcgctcaaag ccggaaacga
      961 gatcttcgat tgcggaattc atggcgcccc ctgctgccga aagcgagcgt ccagtctacg
     1021 ccggatcggc gtgggcgcga caagccgccg cgcgggtccg aacacgtcag ccggtcgcgg
     1081 ccggcgggaa ctgcaagcgg aattccgcgc ccggctcgca gttacgcacg tcgacgcgcg
     1141 cgtccatcgc agcggccagc tgccgcacga gcgcgagccc gatgcccgtc ccgacggttt
     1201 cgcgcgtcag ctcgttctcg gcgcggtaga agagctcgaa gatctttttc atttgcgtct
     1261 tcggcacgcc ggggccgaag tcgcgcacgg tcagcaacac tttgccgtca ccgccgcgcc
     1321 gcgccgcgat ctcgacggtc ttcgtcgtcg cgcccgcgga aaacttcagc gcgttgtcga
     1381 cgaggttgat gaagatctgc gcgaagcagt ccgcgtcggc gacgacggcc gtgccctcgg
     1441 gagtttcgtt gcgcagcgtc agcgtgaacc ccgcgcgctc gacttgcgtc gcgaccttcg
     1501 agcgcacgag atcgagcaac tctgccaccg tgacgctgcg tagatcgaaa cgctgcgtgt
     1561 tgcgtgtcag ccgcgcgagt tgcagcacgt tctcgatcaa tcgcgacagg cgctcgctct
     1621 cgctgtggat gtagtcgtaa tacgtctgcc gcttggcgtc gtcggcccaa ccggccttca
     1681 gcatctcgcc gtacatgcgg atcgaggtca gcggcgtctt cagctcgtgg ctgacggccg
     1741 agacaaagtc ttgctgctgg cgagcgagcc gcatctgacc gacgccgaag cggtacatga
     1801 aaatgaatcc gccgacgagc acggccccga gcgtcacggc gacccaagcg agcagtgcgg
     1861 tgccgggggc ccgcggcaag cggtcgacga cgaaggcgag ctcgaggtcg ccgaacggcg
     1921 gcgacaaccg tgcgcggtgc aacacggtcc cggtaagctc cgtcgagcgc gcattctcct
     1981 cgctcgagat cagcgggcca aagacctccc acggccgacc ctgcagatca acgttgagcc
     2041 gggcgacgct gccgacgttg ctggcgcggt acggagaatc gataacaccc atgacgaacg
     2101 ctgcgcgctc gacgagcgcg ccctgcacgt agcgttgccc gtcgcgccac acgttgcgaa
     2161 acagcacgag gtgctcgtca tcgagcacgc cgagctcgaa cgggtcgacc tcgctcgcga
     2221 acatgcgcac gctgacctcg gccttcgcgt ccgcgaccgc gcccaactcg gccgcatcct
     2281 gctcggcagc gggctcggcg acgagcgatt gctcgagtcg cttctggcgc tgctcggcga
     2341 aggtcgcgac cacggtcacg agctgcgcgg ccggtggagc aacactcggc gcggcgggcg
     2401 cgacgacttc gcgcgccgca ccggcgtcgt cgtcgcgggc gccgacggcg cggcgcagag
     2461 gcggcgcgtc gacggaaaga cgatcgaagc cttcttgcac aaacgaggcc gacgcgctcg
     2521 cgggactcga ggcgttgagc cgccgctggc tccgctcgcg ctcgacttcg gccggtgcag
     2581 gcgcgggctc gtcgcgcgga ccacgcacga gctgattgcg gacgagcagg tcgcgcactt
     2641 gcgccacgcg ctcggcacgc gcggcctcct cgcccggcgc aatgccgtaa ttcgacgccg
     2701 cgacgccggc ctcgggcagc agcggcgtcg tcagcttgcc cgcggcgtcg acctgaaagt
     2761 aacccagcac tccggggatc gcgctttcga cggggaacgc cgacaacggc gagcgctgca
     2821 cgaaattggc ggcggcgtcg ccttcgacga cgaggaacga gtacgcgccg aacgagcgcg
     2881 cgtcctcggt gccgatggcc gcacgcagtg ttgcgtcgac gcgcgccgcg agctcctcgg
     2941 ccgcaacctg cgtgctgcga aacgcctgcc acttgagctg gccgtacgct tgcacgatca
     3001 gcacgccggc cgggagggcc agcgccagga aaaaagcggc gagaacgagc ttcaaggcac
     3061 gttcgtcgaa acgcttcagc acgcgtttca tgcgctgacc acgagccggt aacccgtgcc
     3121 gcgcacggtg atcaaattgc gcggctcctt ggcgtcgacc tcgatcttgc ggcgcagctt
     3181 cgcgatgtgg atgtcgacgg tacgcgtctc gatctcggcc gagcggtcgt aaccccaaac
     3241 gcgcgtcagc agctcgtcgc gcgacacggg gcgctcggtg ttgcgctgca agtacgcgag
     3301 gatctcgatc tcgcggcgcg tgaacgtcaa cggctccgag ccgcggcggc ccgagagatt
     3361 acgcgtatcg atcgcgacgt cgccggcgag cttgatctcg gcctgcgagg cggcgcccgt
     3421 gcgcgagcgg cgcaggacgg ctttgacgcg cagcacgag
//
LOCUS       DATLEE010000758         4917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8942464, whole genome shotgun sequence.
ACCESSION   DATLEE010000758 DATLEE010000000
VERSION     DATLEE010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8942464"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VM692_16705"
     CDS             complement(<1..587)
                     /locus_tag="VM692_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53872.1"
                     /translation="MQHPPITLRYFDARGRAQFLRHYFRARKVSFTDDRVPLAPDFAP
                     WKAVRDDRTRTGPFHKLPVLHWGDRLIAETLMIASFVHEVSGDASSLNDDDNLRHGML
                     TSSLYHDVMTPIGTLLWAEFVSPGADVGAVAKRTLERLELHLRALDQSLGEWRWIDKA
                     RNRRIMLVDCLLWEELHVAQQVFGPRLSLGQMPMLA"
     gene            complement(604..1824)
                     /locus_tag="VM692_16710"
     CDS             complement(604..1824)
                     /locus_tag="VM692_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53873.1"
                     /translation="MSRITFATALLLGATAFAGHAQQHKRFMGGPVTLVDQGSFFVGG
                     VTKVSEYAAVPGAPPGQPTPPRTPQQITIGQMYVQFQIPQRLSTPGWPVIMVHGSTHT
                     GAALEPTPDGREGWYPYFVRKGVATYVVDQSGRGRSGFDQSVLNEAEALMLAGNREAA
                     TARLPTMQRITDNGAWQAWFGHLLPADATIVNGRLVSHGSADDPQGPTVDGQPHVAPQ
                     FPLAAVAEYYKQLVPNAEATLPGSICMTCMPQEVAPANTWTPQNLALLVERLGGAIVA
                     THSQSGIMGHHMIRILKERGHADLVKGLITVEGGCSLPQSGLTAADFDAIPYLAIKGD
                     YTPTSQQCQESVDAINARRAQGQGTAKAEYLKLDEHGMLGVTHMMMLDANNLQIADLM
                     LEWVGANVARAPRR"
     gene            1991..2188
                     /locus_tag="VM692_16715"
     CDS             1991..2188
                     /locus_tag="VM692_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53874.1"
                     /translation="MKAALPQAPTGWLIGGYEVVSAGRRGSASSAAAHSLVVTVEGDP
                     ARIDSLLGSIDFPALAATLAP"
     gene            complement(2204..>4917)
                     /locus_tag="VM692_16720"
     CDS             complement(2204..>4917)
                     /locus_tag="VM692_16720"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HUQ53875.1"
                     /translation="LAWPGVTTVDSPGGTLALIGTFDAYRYDGSGLLETLGRTADFTV
                     NGGGARFELAVQELVLTPTPASGGTLRGTGPVSLANRTADLAVVADDFDPAWLLADWT
                     GRLDGTAKLKAALVPQPNATLDAIELAGELRGYPVTLRGSAALPEPGRFELAALRLES
                     GDNFVVLTGTLDATRLDLAVEADLDKLDMLVPNVAGAVAGAVTVDGTWLAPHARGNLT
                     ATGLAYAGVTVERLDVDGEAGLAPDARLALTVDVVRAARDPVSVGNVRAVANGTTASH
                     TARVEGGTGDLRATVAVTGGAVGNDGWRGTIERLDVEEPRFGPWQLDAPAALAFGRGF
                     VTLANSCLLHTSQARWCTQLDVRGRREDSLVVSGQNFDLATLRPLLPPALELNGVYQV
                     SGALLDLMGEPRGALAVVSSDTRARIEFGESQAFVAELDRVQAGLTLTDGRLELTSAV
                     RSTSGGRAEAVATIADTRERDSAVDGRLHVEWPDLAVLTLLSPQLGEVGGALAADLTA
                     GGTVAEPTVDGRAVVSNGRVVVPQWGLVVERIEATASSSDGRALDIDATGYAGDGALT
                     LEGTTQLDPEAGWPTQLTLRGDAVRVVQRADAEIVATPDLRVEVALPAITVTGSVLVP
                     RASVRVDALPAQAVTPSPDAVVHGDERETQRRPLEVRSSVQLTLGDDVRYAGLNLATT
                     VGGELRLATEPNRSANATGTLRLAGTYDAYGQRLELERGQLLFSGPLDDPGLDVRAVR
                     KLEATAAATSTQATEVGVELTGTLRAPRTRIVSTPAMSEADALSYLLFGRPATGGNAG
                     LGSEETSALQAAALSLGLQQALPVVQRLGNSLGLDELTVQSTTTDAGELMAGKYLSPK
                     VYIRYSYGLFNRIGGLLLRFKVNERLSIETRSGDQKSMDLLYTVEKN"
BASE COUNT          730 a         1702 c         1707 g          778 t
ORIGIN      
        1 gcgagcatcg gcatctgccc taacgacagg cgcggtccga acacttgctg cgctacgtgg
       61 agctcctccc agagcaggca gtcgacgagc atgatgcgcc ggttgcgcgc cttgtcgatc
      121 cagcgccatt cgccgagcga ctggtcgagt gcacgcagat gcagctcgag ccgctcgagc
      181 gtccgcttcg cgacggcgcc gacgtcggcc cccggcgaca cgaactccgc ccagagcaac
      241 gtgccgatcg gcgtcatgac gtcgtggtac aagctcgacg tcagcatccc gtgacgcagg
      301 ttgtcgtcat cgttcaacga gctcgcgtcg cccgagacct cgtgcacgaa cgacgcaatc
      361 atcagcgtct cggcgatcag ccggtcgccc cagtgcagca ccggcagctt gtggaacggg
      421 cctgtgcgcg tgcgatcgtc gcgcacggct ttccacggcg cgaaatcggg cgccagcggc
      481 acgcgatcgt ccgtgaacga gaccttgcgc gcgcgaaaat aatggcgcag gaattgcgcg
      541 cggccgcgcg catcgaagta gcgaagcgta atcggcggat gttgcattct gcgtggtccc
      601 gtatcagcgg cgcggtgcgc gtgcgacgtt cgcgccgacc cattcgagca tgaggtcggc
      661 gatctgaagg ttgttggcgt cgagcatcat catgtgcgtg acaccgagca tgccgtgctc
      721 gtcgagcttc aggtactcgg ccttcgccgt tccctgtcct tgcgcgcggc gcgcgttgat
      781 tgcgtcgacc gattcctggc attgctggct cgtcggcgtg tagtcgcctt tgatcgcgag
      841 gtacggaatc gcgtcgaaat cggcggccgt cagaccgctc tgcggcagcg agcagccgcc
      901 ttcgaccgtg atcaagcctt tgacgagatc cgcatgaccg cgctccttga ggatgcgaat
      961 catgtgatgg cccatgatgc cggactgcga gtgcgtcgcg acgatcgctc cgccgaggcg
     1021 ctcgacgagc agggcgaggt tttgcggcgt ccaagtattg gccggcgcaa cttcctgcgg
     1081 catgcacgtc atgcagatcg agcccggcag cgtcgcttcg gcgttcggca cgagctgctt
     1141 gtagtactcg gcgacggccg cgagcgggaa ctgcggcgcc acgtgcggtt gcccatcgac
     1201 cgtcgggcct tgcgggtcgt cggcggagcc gtgcgacacg agccggccgt tgacgatcgt
     1261 cgcgtcggcg ggcagcagat gcccgaacca cgcttgccag gcgccgttgt ccgtgatgcg
     1321 ttgcatcgtc ggcaagcgcg ccgtcgcggc ctcgcgattg cccgcgagca tcagggcttc
     1381 ggcctcgttc agcacggact gatcgaagcc cgagcgcccg cggcccgatt ggtcgacgac
     1441 gtacgtcgcg acgcctttgc gcacgaaata cggataccag ccctcgcggc cgtcgggcgt
     1501 cggctcgagc gcggcgcccg tgtgtgtcga gccgtgcacc atgatcacgg gccaacccgg
     1561 cgtggatagc cgctggggaa tctggaactg cacgtacatc tggccgatcg tgatctgctg
     1621 cggtgtgcgc ggcggcgtcg gttggccggg cggggcgccg ggaacggcgg cgtactcgct
     1681 gaccttcgtg actcccccga cgaagaagct gccctggtcg acgagcgtga ccgggcctcc
     1741 catgaaccgc ttgtgctgtt gggcgtggcc tgcgaaggcc gtcgccccga gcagcagcgc
     1801 ggtggcgaag gtgatgcggg acatgggtcg aaacctctta tgaagggtcc ttggctgcgg
     1861 cgcagcatgc cacgccgcga gcgttcccga aatgcaccgt tcagccctcg caaccctcct
     1921 ggggggcgag taggctcttg ccgacccata acaaggagaa caatccaatg ggtcccgcgt
     1981 cttcggtgcc gtgaaagccg cgctgccgca ggccccgaca ggttggctga tcggcggcta
     2041 cgaagtcgtg agcgccggcc gccgcggtag cgcgtcatcg gctgccgctc acagcctcgt
     2101 cgtaaccgtg gagggcgatc ccgcccggat cgattcgctg ctcggctcga tcgacttccc
     2161 ggcgctcgcg gcgacgctcg cgccctgatt tgcaacccgt tcgtcagttc ttctcgacgg
     2221 tgtacaagag gtccatcgac ttctgatcgc ccgagcgtgt ctcgatgctc aagcgctcgt
     2281 tgaccttgaa gcgcagcagc aagccgccga tgcgattgaa caagccgtag ctgtagcgga
     2341 tgtagacctt cgggctcaaa tatttacccg ccatgagctc gccggcgtcc gtcgtcgtcg
     2401 actgcacggt cagctcgtcg agaccgagcg agttgccgag gcgctgcacg acgggcaacg
     2461 cctgttgcag cccgagcgac agcgcggccg cctgtagggc cgacgtttcc tcgctgccga
     2521 ggccggcgtt gccgccggtc gcgggccgcc cgaacaagag atacgacagc gcgtcggcct
     2581 cgctcatcgc gggcgtcgac acgatgcgcg tccgcggcgc gcgcagcgtg cccgtgagct
     2641 cgacgccgac ttccgtcgcc tgagtcgacg tggcggccgc ggttgcctcg agcttgcgca
     2701 cggcgcgcac gtcgagcccc gggtcgtcga gcgggccgct gaacaacagt tggccgcgct
     2761 cgagctcgag ccgctgcccg tacgcatcgt aagtgccggc gaggcgcagc gtcccggtcg
     2821 cgttcgcgga gcggttcggc tcggtcgcga ggcgcagctc gccgccgacc gtggtggcga
     2881 ggttgaggcc ggcgtaacgc acgtcgtcgc cgagcgtcaa ttgcactgac gaacggactt
     2941 cgagcggccg cctttgcgtc tcgcgctcgt cgccgtgcac gacggcatcc ggcgaaggcg
     3001 tcacggcttg ggccggcaac gcatcgaccc tcacggaggc gcgcggcacg agaacgctgc
     3061 cggtgaccgt gattgccggc aacgcgacct cgacgcgcaa gtccggcgtc gcgacgatct
     3121 ccgcgtcggc gcgttgcacg acgcgcacgg cgtcgccgcg taacgtgagt tgcgtcggcc
     3181 agcccgcttc agggtcgagc tgtgtcgtgc cctcgagcgt caacgcgccg tcgccggcgt
     3241 agccggtcgc gtcgatgtcg agcgcacggc cgtcgctgct cgaggccgtc gcctcgatgc
     3301 gctcgacgac gagcccccat tgcggaacga cgacacgccc gttcgagacg acggcgcggc
     3361 cgtcgaccgt cggctcggcg acggtgccgc cggccgtgag atccgcggcc agcgcgccgc
     3421 cgacttcgcc gagctgcggc gacagcagcg tcaacactgc gagatcgggc cattcgacgt
     3481 gtagccggcc gtcgaccgcc gaatcccgct cgcgcgtgtc ggcgatcgtc gcgactgcct
     3541 cggcgcggcc gccgctcgtg ctgcgcacgg ccgatgtgag ctcgagtcgc ccgtccgtca
     3601 gcgtcaggcc ggcttgcaca cggtcgagct cggcgacgaa cgcctgcgac tcgccgaact
     3661 cgatgcgtgc tcgcgtatcg ctgctgacga ccgcaagcgc gccgcgcggc tcgcccatga
     3721 ggtccagcag cgcgcccgac acctgataga cgccgttgag ctcgagcgca ggcggcaaca
     3781 gcgggcgcag cgtcgcaaga tcgaagttct ggccagagac gaccaagctg tcttcacgcc
     3841 ggccgcggac gtcgagctgc gtgcaccagc gcgcctgcga cgtgtgcagc agacagctgt
     3901 ttgcgagcgt cacgaatccg cggccgaacg cgagcgcggc cggcgcatcg agctgccagg
     3961 gcccgaaccg cggctcttcg acgtcgagcc gctcgatcgt tccgcgccaa ccgtcgttgc
     4021 cgacggcgcc gcctgtcacc gctaccgtcg cgcgcaagtc gcccgtgccg ccttcgacgc
     4081 gcgccgtgtg cgaggccgtc gtaccgttcg cgacggcgcg aacgttgccg acgctcacgg
     4141 ggtcgcgcgc ggcgcgcacg acgtcgaccg tgagcgcgag ccgcgcgtcg ggcgccaagc
     4201 cggcctcgcc gtcgacgtcg agccgctcga ccgtgacgcc tgcatacgcg agccccgtgg
     4261 ccgtgaggtt gccgcgtgcg tgcggcgcga gccacgtgcc gtcgacggtc acggctcccg
     4321 ctacggcacc ggcgacgttg ggcacgagca tgtcgagctt gtcgaggtcg gcctcgacgg
     4381 cgagatcgag cctcgtggcg tcgagcgttc ccgtcagcac gacgaagttg tcccccgact
     4441 cgagccgtag cgcggcgagc tcgaagcggc cgggctcggg cagcgccgcc gagccccgca
     4501 gcgtcacggg ataaccgcgc agctcgccgg ccagctcgat tgcatcgagc gtcgcgttcg
     4561 gctgcggcac gagcgccgct ttgagcttcg cggtcccgtc gagccgtccc gtccaatcgg
     4621 ccaggagcca ggcaggatcg aagtcatccg cgacgacggc gaggtctgcg gtgcggttcg
     4681 ccaagctgac ggggccggta ccgcgcaacg ttccgccgct cgcaggcgtc ggcgtgagca
     4741 cgagctcttg gacggcgagc tcgaaacgcg cgccgccgcc gttgacggtg aagtcggccg
     4801 ttcggcccaa cgtttcgagc aaacccgagc cgtcgtagcg gtaggcatcg aacgtcccga
     4861 tcagcgcaag cgtcccgccg gggctgtcga cggtcgtgac gcccggccac gcgagcg
//
LOCUS       DATLEE010000759         2475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8000183, whole genome shotgun sequence.
ACCESSION   DATLEE010000759 DATLEE010000000
VERSION     DATLEE010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8000183"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1477
                     /locus_tag="VM692_16725"
     CDS             104..1477
                     /locus_tag="VM692_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HUQ53876.1"
                     /translation="MSVTEALAEYVTHSTFEAIPRDVRDEAKRAIFNYLGCALGGSVE
                     SALDTAIRTFAPYSGNATACVLGRAERFDALHAALLNGIGSHVHEYDDTLPKNFIHPS
                     VPVGSALFAYASANPVSGRDFVHAFILGFEVESRIGNAVYPAHYSAGWHITSTTGVFG
                     AAAAIGKLLGLSTKQMVWAFGLAATQAAGLREMFGSMAKSFQPGRAAQNGYTAALLGK
                     ADFTAGEHALEGPRGFAAVTAAQYDLNKITAGLGKDFELRDNAYKPYACGLVVHPTID
                     GCSQLHREHHPSPDSIAGVRLRVAPLVLDLCNKSNLQRALESKYSIYHAAAIGLVRGK
                     GGLQEFTDEAIADPALQRVRAVTTAVGDDTISEDQVHIEVVLRNGETLSKFVEQSLGN
                     VHRPLSNEQLTEKFVDQAVLALPRHQVDAVVEQCWRIDALPSAGDLARATSPKSAGTL
                     RSGRTTH"
     gene            1482..>2475
                     /locus_tag="VM692_16730"
     CDS             1482..>2475
                     /locus_tag="VM692_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUQ53877.1"
                     /translation="MRATRRTVGLSLLAATVALAAGCGGQAGGSSTEVRIPLGAGGVG
                     FLPLLVMRDRRLIEKHGQAAGLADLTVRWIELGGPAVMNDALLSGSVDFIAAGPPAFI
                     TLWDRTRGSADVRGVAAMSSLPMYLNTTRSSLTTLDDLTATDKIAVTGVKVSIPSINM
                     QMYAAQRYGAAEAFRFDRYTVTMTHADALIALLSGSNQINAHFTSPPFHQREIKDPRV
                     RTVLNTDDIMGGATTFTMLSTTARYREANPLAYRAVLAALEEANELIRREPQAAAQIL
                     FAAESAAGFSVDEIVEVLRDPDIRFTTTPQNTEKYAEFMLEIGSIENRPESWRDL"
BASE COUNT          430 a          847 c          789 g          409 t
ORIGIN      
        1 gagccactac ctgccgccga tctatagcgt gcgtttcgcg aattgagcca aagctcgcgg
       61 caccgcgagc aacgcttggc ctcgtcaatc agttggagct ccgatgtccg tcaccgaagc
      121 gctcgccgag tacgtcactc acagcacgtt cgaggccatt ccgcgcgacg tccgtgacga
      181 ggcgaagcgc gccatcttca actatctcgg ctgcgcgctc ggcggctcgg tggaatccgc
      241 gctcgacacc gcgatccgga cgttcgcgcc ctactccggt aacgcaacgg cgtgcgtgct
      301 cggccgcgcg gagcgcttcg acgcgctgca cgcggcgctt ttgaacggta tcggctcgca
      361 cgtgcacgag tacgacgaca cgctgccgaa gaatttcatt cacccgagcg tgcccgtcgg
      421 ctccgcgcta tttgcctacg ccagcgcgaa ccccgtgtcc ggccgcgact tcgtgcacgc
      481 gtttatcctc ggcttcgagg tcgagtcgcg catcgggaat gccgtctacc cggcgcatta
      541 ctcggccggt tggcacatca cgtcgacgac aggggtgttc ggcgccgcgg cggcgatagg
      601 caagctgctg gggctcagca cgaaacaaat ggtctgggcg ttcggcctcg ccgcgacgca
      661 agccgccggc ctccgcgaaa tgttcggctc gatggcgaag agctttcagc ccggtcgcgc
      721 cgcgcagaac ggctacacgg ccgcgttgct cggcaaagcc gactttacgg ccggcgagca
      781 cgcgctcgaa ggtccgcgcg gcttcgctgc cgtgacggct gcgcagtacg acctcaacaa
      841 aattactgcg ggcctcggca aggacttcga gctgcgcgac aacgcgtaca agccctatgc
      901 ctgcggcctc gtcgtgcacc cgacgatcga cggctgcagc caattgcatc gcgagcatca
      961 tccgtcgccg gattcgattg ccggagtgcg cctgcgcgtc gcaccgctcg tgctcgacct
     1021 ctgcaacaag agcaacttgc agcgcgcgct cgagagcaag tattcgattt atcacgccgc
     1081 cgccatcggc ctcgttcgcg gcaaaggcgg cttgcaggaa ttcaccgacg aggcgatcgc
     1141 ggaccccgcg ctgcagcgcg tgcgcgccgt gacgacggcc gtcggcgacg acacgatcag
     1201 cgaggatcaa gtccacatcg aggtcgtgtt gcgcaacggc gaaacgttgt cgaagttcgt
     1261 cgagcagtca ctcggcaacg tgcatcggcc gctgtcgaac gagcaactca ccgagaagtt
     1321 cgtcgatcaa gcggtgctcg cgttgccgcg ccaccaggtc gacgccgtcg tcgagcagtg
     1381 ctggcgcatt gacgctttgc ccagcgccgg cgacctcgca cgcgcgacga gtccgaagtc
     1441 agccggcacg ctgcgctccg gccgcacgac gcactgacgc gatgcgcgca actcgccgca
     1501 cggtaggtct atcgctcctc gcggccactg tcgctctcgc agcgggttgc ggcggccaag
     1561 cgggcggcag ctcgacggaa gtccgcatcc ccttgggggc cggcggcgtc ggcttcctgc
     1621 cgctgctcgt catgcgcgac cgcagactca tcgagaagca cgggcaagcc gcgggcctgg
     1681 cggatctcac ggtgcgttgg atcgagctcg gcggcccggc cgtcatgaac gacgcgctgc
     1741 tgtcgggctc cgtcgacttc atcgccgcgg gcccaccggc gttcatcacg ctctgggatc
     1801 gcacgcgcgg ctcggccgac gtgcgcggtg tcgccgcgat gtcgtcgctg ccgatgtatc
     1861 tgaacacgac gagatcttcg ctgacgacgc tcgacgatct cacggcgacg gacaagatcg
     1921 ccgtcacggg cgtcaaggtg tcgatcccgt cgatcaacat gcagatgtac gcggcgcagc
     1981 gctatggcgc agcagaagcg ttccgcttcg atcgctacac cgtgacgatg acgcatgccg
     2041 atgcactgat cgcgctgctc tctggctcga accagatcaa cgcgcacttc acgtcgccgc
     2101 ctttccacca acgcgagatc aaggatccgc gcgtgcgcac ggtgctgaac accgacgaca
     2161 tcatgggcgg cgcgacgacg ttcacgatgc tgtcgacgac ggcgcgttac cgcgaggcga
     2221 acccgctagc gtacagagcc gtgctcgccg cgctcgaaga ggccaacgag ctgatccgcc
     2281 gggagccgca agcggcagcg cagatcttgt tcgcagcgga gtcggcggcc gggttctccg
     2341 tcgacgaaat cgtcgaggtc ttgcgcgacc cagacattcg cttcacgacg acgccccaga
     2401 acacggaaaa gtacgccgag ttcatgcttg agatcggctc gatcgagaac cggccggaat
     2461 cgtggcgcga tctgt
//
LOCUS       DATLEE010000760         5531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10246710, whole genome shotgun sequence.
ACCESSION   DATLEE010000760 DATLEE010000000
VERSION     DATLEE010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10246710"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1614)
                     /locus_tag="VM692_16735"
     CDS             complement(106..1614)
                     /locus_tag="VM692_16735"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ53878.1"
                     /translation="MKKLALTALCATYLSGYALAQDAKTVIADAQRALGNPQSITYSG
                     SAHDVAFQQCGSNAAALICYGTHDPMRPIMEYVRVIDLNGPLSRHTGKTMNPAGGGNT
                     VPAPGVFFQQVTAQQADVSQPWAQSLEFYITPWGFLKGAAANSTTAARRNVDGKSYTV
                     LTWSPTVKAPSGKAYTVNGYVNSANIVERVETWLGENIMGDMQIVADYKDWRDFGGVQ
                     IPTHIVQTRGGWPFFDVEVASARGNPPNLAQLAVDTRPPPPAGGPGGGGGPPGGAPPA
                     MTVTAEKLADGVFRYTTGAGSYDSLVVEFNNYIMMLEAGQSQARAAAYVAEIKKQFPD
                     KPIRYVMVTHPHADHTGGLPVLVAEGATLVVQENSREFYERALNTPRTLLDDALAKAP
                     KRAKIETVADKKVYTDGTHTVEMYHIYPAPHSNALLIAYLPKEKVLFQGDFSVNPAQG
                     GGTQPANDHVQALVPALAKLGITDYTRYINVHASTAPQTKADVTAAMNALPR"
     gene            complement(1736..2215)
                     /locus_tag="VM692_16740"
     CDS             complement(1736..2215)
                     /locus_tag="VM692_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ53879.1"
                     /translation="MRLLAGAVALLAVSTMANAAEPVVAISPEAQRALLASPDPQLAA
                     NKKLAWDMWRNFLQAGVIDAADTYLAPEYHQHNPNADTGRDGVKAYFRAQNRKPDPVK
                     DSIDRLVSIVAERDLVVFALVREGMDKDGKPYTTTWFDMFRIANGKIVEHWDTATKP"
     gene            2401..2988
                     /locus_tag="VM692_16745"
     CDS             2401..2988
                     /locus_tag="VM692_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HUQ53880.1"
                     /translation="MNGPSRTLLVMTVLLVPAVSMAQQQSQPFSYTYAEIGYDESDFD
                     LGGGGDIDGDGLTLSGSYAINDDWHAYASYGTADLDFGIDVDTLAIGAGYRYPLQDDV
                     DIYGRVLYISSDVNLPGPGDGDEDGLGLQVRIRYRVSDQFEVEGGIQHIDVFDSDTSL
                     QAMARYHFTENFSAGIGLTFAGDTDTFGINARYSF"
     gene            3084..4169
                     /locus_tag="VM692_16750"
     CDS             3084..4169
                     /locus_tag="VM692_16750"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53881.1"
                     /translation="MQHDTSRFAATAALLAALLVHAPQVAAHHSTAEYDAASIVEARG
                     QVVSVIWRNPHVRFQVSTKAIDGNDKLWEIESADLTRLDRAGLPRNLLNVGDTITFAG
                     NPSTRTERRMYVTNLLVADGREILLRGNVKPRFAIDRAVNTDAIPTRPGANAATPSGN
                     KFFGRVFVPSREGQAPAWLRDPPLTAAGQAGRAAYDEVVDDPVLGCVSPGMPRVMLRS
                     GPYAVRFIERGSDLILQNEWFEIDRVIHMDKGPAANEPYTPLGYSAGRWDGDALVITT
                     THIDWPYFELYGLVGVPQSRQMRIVERFTPRDGNATLQYDWSATDPVNLKETISYENY
                     VTFRFNPSLDFLPYDCVEDERRARTTR"
     gene            4252..5241
                     /locus_tag="VM692_16755"
     CDS             4252..5241
                     /locus_tag="VM692_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ53882.1"
                     /translation="MLKASIGSATALLALCGTAFAQPAGITAEMINTTLPLEGAPLAV
                     PGPYQTMSEPTFGKPGLILFRPTNLDAFPARDKLPVLVWGNGGCGIDTTRYGGFLSTI
                     VSHGFIAIGTVPLEGAAPPQGAAPQQGGAPQAGAPRRQATADDLRGAIDWAFAENGRA
                     GSPLNGKIDTTQVAVMGQSCGGFLSIGLGADPRVDTIGVFNSGVTPPNPNAPANPNAQ
                     ANASRPTMDSLKNLHGPVLLINGHERDFLMGASKATFDAINHVPAFYGARHGAGHTAT
                     VFHPGGGEYANVASNWLKWQFKKDAAASKMFVGDDCSLCTNSNWDTESKGFGK"
BASE COUNT          930 a         1817 c         1797 g          987 t
ORIGIN      
        1 cgccgtcccg tcgacgccgt cgctgtagcc cccgagccta agcgtcggcg aaaaaaaacg
       61 gcggccacga gggccgccgt tccaaggcgc aaagaatctg ctgcgttatc gcggcagcgc
      121 gttcatcgcc gccgtgacat ccgccttggt ctgcggagcc gtggaagcgt gcacgttgat
      181 gtagcgcgtg tagtcggtga tgccgagctt cgcgagcgcc ggcacgagcg cctgaacgtg
      241 atcgttcgcg ggctgagtgc cgccgccctg cgccgggttc accgagaagt cgccctggaa
      301 gagcaccttc tccttcggca ggtacgcgat cagcaacgcg ttcgagtgcg gcgccggata
      361 gatgtggtac atctcgacgg tgtgcgtgcc gtccgtgtag accttcttgt ccgcgaccgt
      421 ctcgatcttg gcccgcttcg gcgctttcgc gagcgcgtca tcgagcagcg tgcgcggcgt
      481 gttcaacgca cgctcgtaga actcgcgcga gttctcctgc acgaccagcg tggcgccctc
      541 ggcgacgagc accggtaagc cgcccgtgtg atccgcgtgc gggtgcgtga ccatgacgta
      601 gcggatcggc ttgtccggga actgcttctt gatctccgcg acgtaggccg cggcgcgggc
      661 ctgactctgg ccagcctcga gcatcatgat gtagttattg aactcgacga ccagcgagtc
      721 gtagctgccg gcacccgtcg tgtagcggaa cacgccgtcg gccagctttt cggccgtgac
      781 cgtcatcgcg ggcggtgcgc cgcccggagg accaccaccg ccgccggggc cgcccgccgg
      841 aggcggcggg cgcgtgtcga cggccaattg cgcgagattc ggcggattgc cgcgcgcact
      901 cgcaacctcg acgtcgaaga acggccagcc gccgcgcgtc tgcacgatgt gcgtcggaat
      961 ttggacgccg ccgaaatcgc gccagtcctt gtagtccgcg acgatctgca tgtcgcccat
     1021 gatgttctcg ccgagccacg tctcgacgcg ctcgacgatg ttggcggagt tgacgtagcc
     1081 gttcaccgtg tacgccttgc ctgaaggcgc tttgacggtc gggctccacg tcagcacggt
     1141 gtagctcttg ccgtcgacgt tacggcgagc tgccgttgtg ctgttggcgg cggcaccctt
     1201 caagaagccc cacggcgtga tgtagaactc gagcgactgc gcccacggct gcgagacgtc
     1261 ggcctgttgt gccgtgactt gctgaaagaa cacgcccgga gccggcacgg tgttgccgcc
     1321 gcccgcgggg ttcatcgtct tgcccgtgtg ccgcgacagc ggcccattga gatcgatgac
     1381 gcgcacgtac tccatgatcg gccgcatcgg atcgtgcgtg ccgtagcaga tcagagcggc
     1441 tgcgttggag ccgcactgct ggaacgcgac gtcgtgcgcc gagcccgagt acgtgatgga
     1501 ttgcggattg cccaacgcgc gctgcgcgtc ggcgatgacg gttttcgcgt cttgcgcgag
     1561 cgcgtacccg gacaggtacg tcgcgcagag cgcggtcagc gctaatttct tcacggctag
     1621 tcccccttcg ttcgttattg agtgagcgcg gaatcgcgtg ccagcgaatc ttaccgacga
     1681 gtcagtcggc ggcgccatgg tcgcgggcgt gccgaccgac tcgtcttgag atcacttaag
     1741 gtttcgttgc ggtatcccag tgctcgacga tcttgccgtt ggcgatgcgg aacatgtcga
     1801 accaggtggt cgtgtacggc ttgccgtcct tgtccatgcc ttcgcgcacg agcgcgaaca
     1861 cgacgagatc gcgctcggcg acgatcgaca ctaggcggtc gatcgagtcc ttgacgggat
     1921 cgggcttgcg attctgtgcg cgaaagtacg ccttgacgcc gtcgcgaccg gtgtccgcgt
     1981 tcgggttgtg ctggtggtac tcgggcgcga gatacgtgtc ggccgcgtcg atgacgccgg
     2041 cttgcaagaa gttgcgccac atgtcccacg cgagcttctt gttggccgcg agttgcgggt
     2101 cggggctcgc gagcaacgcg cgttgcgcct cgggcgagat cgcgacgacg ggctcggcgg
     2161 cattggccat cgtcgacacg gcgagaaggg caacagcccc ggcaagcaat ctcatggaat
     2221 ttttctcctg acgttatggg ttgcgattcg agccagatcg aatcgtgagc ccgattctac
     2281 ctcgtgcggg cgcgcgcgac gctacgcact gtcgccaaag aggtacacgc cgcctgcgca
     2341 cgagtgttct aaaatatcgt ctaacgggcg ttcgctcgcc aattcccaag gagatagagg
     2401 atgaatggac catcgagaac gttgctcgtg atgacggtgt tgctggtacc ggccgtgagc
     2461 atggcgcaac agcagtcaca accgttcagc tacacctacg ctgagatcgg ctacgacgag
     2521 tcagacttcg atctcggcgg cggcggcgac atcgatggcg acgggttgac gttatccggc
     2581 tcgtacgcga tcaacgacga ctggcacgcc tacgcgtcct acggcaccgc cgacctcgac
     2641 ttcggcatcg acgtcgacac gctggcgatc ggcgccggct atcgctatcc gctgcaggac
     2701 gacgtcgaca tctacggccg cgttctctac atcagcagcg acgtgaatct gccgggcccc
     2761 ggcgacggcg acgaggacgg tctcgggctg caagtgcgga tccgttatcg cgtcagcgac
     2821 cagttcgaag tcgaaggcgg catccagcac atcgacgtct tcgacagcga cacgtcgctg
     2881 caagcgatgg cccgctacca cttcaccgag aacttctcgg ccggcatcgg cctgacgttc
     2941 gcaggcgaca ccgacacgtt cggcatcaac gcccgctatt cgttctgatc gctccggccg
     3001 cgcgcgcagc gctcctgcgc gcgcggctcc tttatgaact gagctagaat cgactcgtcg
     3061 ccacgagtgc gaggcccacg ccgatgcagc acgacacgag tcgattcgca gcgacagcgg
     3121 cacttctcgc cgctttgctc gtccatgcac cgcaggttgc ggcgcatcac tcgacagccg
     3181 aatacgacgc cgcttcgatc gtcgaagcgc gcggccaggt cgtcagcgtc atttggcgca
     3241 atccgcacgt gcggtttcag gtcagcacga aagccatcga cggcaacgac aagctctggg
     3301 agatcgagag cgcggacttg acgcgcctcg atcgcgcagg cctgccgcga aacctgctca
     3361 acgtcggcga tacgatcacg ttcgccggca atccgtccac gcgcaccgag cggcgcatgt
     3421 acgtcacgaa tctgctcgtg gccgacggcc gcgaaatttt gctgcgcggc aacgtcaagc
     3481 cgcgctttgc gatcgatcgt gccgtgaaca ccgacgcgat tccgacgcgc ccgggcgcga
     3541 acgccgcgac accgagcggc aacaaattct tcggccgcgt attcgtgccg tcgcgcgaag
     3601 gccaagcccc cgcgtggcta cgcgatccgc cgctgaccgc cgcgggtcag gccggcagag
     3661 cggcgtacga cgaagtcgtc gacgatcccg tgctcggctg cgtgtcgccg ggtatgccgc
     3721 gcgtcatgct gcgcagcggc ccatatgccg tccgcttcat cgagcgcggc tcggacctta
     3781 tcctgcaaaa cgaatggttc gagatcgacc gcgtcattca catggacaag gggcccgccg
     3841 cgaacgagcc gtacacgccg ctcggctatt cggctggccg ttgggacggc gacgcgctcg
     3901 tgatcacgac gacgcacatc gactggccct acttcgagct ctatggcttg gtcggcgtgc
     3961 cgcagagccg gcagatgcgc atcgtcgagc gcttcacgcc gcgcgacggc aatgcgacgt
     4021 tgcagtacga ctggtcggcg acggaccccg ttaacctcaa agaaacaatt agttacgaaa
     4081 actacgtgac gtttaggttc aatccgtcgc tcgacttctt gccctacgac tgcgtcgagg
     4141 acgagcggcg cgcccgcacc acgcgttgag ccttcacgcg ccgccttgcg gcggccctgt
     4201 ggctgggtga cgatggtggc cattcatcat cgcgctaggg ggaaacgcgt catgctcaaa
     4261 gcttccatcg gttcggcaac ggcgctgctt gccctctgcg gtacggcttt cgcacagccg
     4321 gccggcatca ctgcggaaat gatcaacacg acgttgccgc tcgaaggagc gccgctcgcc
     4381 gttccgggcc cgtatcaaac gatgtccgag ccgacgttcg gcaagccggg cctcatcttg
     4441 tttcggccga cgaatctcga tgccttcccg gcgcgcgaca agctgcccgt cctcgtgtgg
     4501 ggcaacggcg gctgcggcat cgacaccacg cgttacggcg gcttcctctc gacgatcgtg
     4561 tcgcacggat tcattgcgat cggcacggtg ccgctcgagg gcgccgcacc gccgcaaggt
     4621 gctgcgccgc agcaaggtgg cgcgccgcaa gcgggtgcgc cgcgcagaca agcgacggcc
     4681 gatgacctcc gcggcgctat cgattgggcg ttcgccgaga acggccgcgc cggctcgccg
     4741 ctgaacggca agatcgacac gacgcaggtc gccgtgatgg gccaatcgtg cggcggcttc
     4801 ttgtcgatcg gactgggcgc cgacccacgc gtcgacacga tcggcgtgtt caactccggc
     4861 gtaacgccgc cgaatccgaa cgcgcctgcc aatccgaatg ctcaggcgaa cgcctcgcgc
     4921 ccgacgatgg actcgctgaa aaacctgcac ggccccgtgc tgttgatcaa cggccacgag
     4981 cgcgacttct tgatgggcgc ctcgaaggcg accttcgacg cgatcaatca cgtgccggcg
     5041 ttctacggcg cgcggcacgg cgccggccac acggcgaccg tgttccatcc cggcggcggc
     5101 gagtacgcca acgtcgcgtc gaactggctc aagtggcagt tcaagaagga cgcggcagcg
     5161 tcgaagatgt tcgtcggcga cgactgcagt ctctgcacga attcgaactg ggacaccgag
     5221 tcgaaaggct tcgggaagta acggcgctgg cgacggccgg ccgcttgcgg aagaccgtcg
     5281 ttttcgccgc ggcttgcgtc gcggcgtgcg cgcatccggc tttcggccgc gaggcccaac
     5341 aacccgactc gcgcacggtc gcactgacgc cccgccattc agggtgaatt cagcccgctg
     5401 gagtactctc gggctgttgg tcgcgacgag ggggtcgcca tgaaagcgct gctggttttg
     5461 gctgtcgtgg gaacgctcgc cgcgtgctcg agcggcacga gcattcgcgt ctcggatccc
     5521 gatgcccgca t
//
LOCUS       DATLEE010000761         4100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10249944, whole genome shotgun sequence.
ACCESSION   DATLEE010000761 DATLEE010000000
VERSION     DATLEE010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10249944"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VM692_16760"
     CDS             complement(<1..1225)
                     /locus_tag="VM692_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53883.1"
                     /translation="MQTASDVSPARLVRAPNRIEFSLSEALPKLGWVATLDTDQARLV
                     VAHGRAVECRPTFLVEGVWDGPFERGEFHRGAHLFGSGIRLDGDTVYFVPSAALVDRL
                     VYCREGGKLLVSNSVVALLAATGATLDPSHDYLVDAKAMLDGIDRYDPTLHVLHPTIE
                     RFHQVYHKVMTVRGGDIALERTIPVREFTSFHDYHTMLRERLAAIAANAGDAGRRTPV
                     TSFGTLSSGYDSTAVTALIKDLGVQSYFTYVGAWGGADTKGKPEYDTAAIAAALAVDT
                     IAIQDPPAPDAEDELLLRAASPLGQQMPLIAMASYVERHSQVAAVFTGFHGDVVWGLN
                     VPESALSESIVRHGVSGLDLSELRLKSGFCNVAVPFLYAASIKSIAALSRSPEMAPWT
                     LGTSYDRPISRRIVEG"
     gene            complement(1382..3019)
                     /locus_tag="VM692_16765"
     CDS             complement(1382..3019)
                     /locus_tag="VM692_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53884.1"
                     /translation="MRNISDVADTAGARSPSCIEAKVSAALPKLAWVAILDRAQQQLT
                     VLHGRAVECRPRFVVEGVWDGPFDEGEFHNGAHLFGSGVRLDGGAVHFVPSAATVDRL
                     VYCRYGDMILVSNSLLALLAKTGARLDPGHDYLIEARAPIAGVDCYDSAFHVLHPRIR
                     RFHQLYHKVLTVRGEEITCERRIPVRTFASFDDYYGMLRECLAAICANADDAARRTRL
                     ATLGTLSTGYDSTAVCALIKDLGVRRYFTYVGSWGGQSTKDAPEYETAPIAAALDVET
                     IRIEAPREPVSEDELLLRAASPLGQQLPLVAIASYVERNTEVAAVFTGFHGDIVWDLN
                     VPDAAVSEGIVRHDISGLDLSELRLKSGFINVAVPFLHAASIKSIAAVSRSAEMAPWT
                     VGTTYDRPISRRIVETAGVPRERFGFLKGGLFGGSNRPASAKLRARYFQYVNRNVMPL
                     PMFYARVGLDHYTMRLLARAALLVKGRARLDKIRAALLEPFRRFNEGYSWYGRSNLRS
                     TLYSWAAMELVTKLRERGIAEPTAGTAGRGLRSAAVR"
     gene            complement(3110..3742)
                     /locus_tag="VM692_16770"
     CDS             complement(3110..3742)
                     /locus_tag="VM692_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53885.1"
                     /translation="MNMRISALLLAALPTIAAPQTALPPDIHPVTLSRLPPVTPEDLD
                     EEARKLLAARPFTPGPGPTHVTVYSPRDRSLGIPSGENSPIGARQFQLAVLIAAREID
                     QQYEWSAHEPAGRRQGLEQNVIDVVKYDRPVAGLADKDATMITFGRTLLREHKVSSEL
                     WAKMVALLGRQGTVDFMSIMGDYTRVGIMLNAVDQHLPPTREALLPSLDR"
     gene            3858..>4100
                     /locus_tag="VM692_16775"
     CDS             3858..>4100
                     /locus_tag="VM692_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53886.1"
                     /translation="MLRLLRPTLLALVAAAALPAIAQDDVDVFGLEAGEHSVGFQLLE
                     DVDASRVVTGGVRGATHPRPIRTYLWYPAEAPRRAQP"
BASE COUNT          693 a         1368 c         1339 g          700 t
ORIGIN      
        1 ctccttcgac gatgcgccgg ctgatcggcc gatcgtagct cgtgccgagc gtccacggcg
       61 ccatctccgg cgaccgcgac agggctgcga tgctcttgat gctcgccgca tagagaaaag
      121 gcacggcgac gttgcaaaag ccgctcttca atcgcagctc gctcaggtcg agccctgaaa
      181 caccgtgccg gacgatgctc tcggacagag cgctttcggg cacgttgagg ccccatacga
      241 cgtcgccatg gaagcccgtg aagacggctg cgacttgcga gtggcgctcg acgtagctag
      301 ccatcgcgat caacggcatt tgttgaccga gggggctcgc tgcgcgcagc agcaactcgt
      361 cttcggcgtc cggcgccggc gggtcttgga tcgcgatcgt gtcgaccgcg agagccgccg
      421 cgatcgctgc ggtgtcgtac tccggcttgc ctttcgtatc cgcgccgccc caagcgccga
      481 catacgtgaa gtaactttgc acaccgaggt ccttgatcaa cgccgtgacc gccgtcgagt
      541 cgtagccgct cgaaagcgtt ccgaacgacg tgacgggcgt gcggcgcccc gcatcgccgg
      601 cattcgccgc aatggctgcg agccgctcgc gcagcatcgt gtggtagtcg tggaagctcg
      661 tgaactcgcg gacggggatc gtccgctcga gtgcgatgtc gccgccgcgc acggtcatca
      721 ctttgtgata gacctggtgg aagcgctcaa tggttggatg gagcacgtgt agcgtgggat
      781 cgtagcggtc gatgccgtcc aacatcgcct tcgcgtcgac gaggtagtcg tgcgacggat
      841 cgagcgtcgc gcccgtcgct gccaacaacg cgacgacgct gttcgacacc aacagcttgc
      901 cgccctcgcg gcaataaacc aggcggtcta ccaacgcggc cgaaggaacg aagtagaccg
      961 tgtcgccgtc gagccggatg ccggagccga acaggtgcgc gcccctgtga aactcgccgc
     1021 gctcgaacgg gccgtcccaa actccttcga cgagaaaggt cggtcggcat tcgactgcgc
     1081 gcccatgcgc cacgactagc cgagcctggt ccgtgtcgag cgtggctacc cagccgagct
     1141 tcggcaacgc ctcgctcaaa gaaaactcga ttctgttggg cgcgcgcacg agccgcgcgg
     1201 ggctcacgtc ggaagctgtc tgcatggtcc accccgggtc gttgtacgac aagcaaaggg
     1261 cggacgacac cctcgccgtt gccgccccgc gacgtaagct gcaaatagcg cgccagcgaa
     1321 cgcgcgctga agttgcgcta cttccgccgg ccgcgtccgg ccgcggcggc acgacgagcg
     1381 actagcggac ggcagcgctg cggagcccgc ggcctgccgt cccggccgtc ggctcggcga
     1441 tgccgcgctc ccgcagcttc gtgaccagct ccatggccgc ccatgaatag agcgtgctgc
     1501 gcagattgct cctgccgtac cacgaatagc cttcgttgaa ccggcgaaac ggctccagta
     1561 gcgcggctct gatcttgtcg agccgcgccc tgcccttcac gagcagggca gcgcgcgcca
     1621 gcagccgcat cgtgtagtgg tccaggccga cgcgcgcgta gaacatcggc aacggcatga
     1681 cgttgcgatt gacgtactgg aagtagcggg cacgtagctt ggcgctggca ggccgattgc
     1741 tgccgccgaa caaaccgcct ttcaggaagc cgaagcgttc tcgcggcacg ccggcggttt
     1801 cgacgatgcg ccggctgatc ggccggtcgt atgtcgtacc gactgtccac ggcgccattt
     1861 ccgccgaccg cgacacggcc gcgatgctct tgatgctcgc ggcgtgcaag aacggaacgg
     1921 cgacgttgat gaagccgctc ttgaggcgta gctcgctcaa gtcgagcccc gagatatcgt
     1981 gccggacgat gccttccgac actgccgcgt cgggcacgtt gagatcccac acgatgtcgc
     2041 cgtggaagcc cgtgaacacg gcagctacct ccgtgttgcg ctcgacatag cttgcgattg
     2101 cgacgagcgg cagctgttgt cccagcgggc tcgcggcgcg cagcagcaat tcgtcctccg
     2161 agaccggctc tcgcggagcc tcgatgcgga tggtctcgac gtcgagcgcg gccgcgattg
     2221 gcgccgtttc atactcgggg gcgtccttcg tggactgacc gccccacgac cctacgtacg
     2281 tgaagtagcg ccgcacaccg agatctttga tcaacgcgca gacggccgtc gagtcatagc
     2341 ccgtcgaaag cgtgcccagg gtcgcgagcc tcgtgcgccg agcggcgtcg tccgcattgg
     2401 cgcagatcgc cgcgaggcac tcgcgcagca tgccgtagta gtcgtcgaag ctcgcgaacg
     2461 tacggacggg aattcgccgc tcgcacgtga tctcttcgcc ccggaccgtc aacaccttgt
     2521 gatagagctg atggaagcgc ctgatcctcg ggtggagcac gtgaaacgca ctgtcatagc
     2581 aatccacgcc tgcgatcggc gctcgtgctt cgattagata gtcgtgaccc ggatcgagtc
     2641 gcgcaccggt cttcgccaat agcgccaaga ggctgttgga cacgaggatc atgtcgccat
     2701 agcggcagta aacgagtcgg tcgaccgtcg cagccgacgg gacgaagtgg acggctccgc
     2761 catccaggcg cacaccggag ccgaaaagat gcgcgccgtt gtgaaactcg ccttcgtcga
     2821 acggcccgtc ccatactccc tcgacgacga atcgcggccg gcactcgacg gctctgccgt
     2881 gtagcacggt cagctgctgc tgcgcccggt cgagaatggc cacccaggcg agcttcggta
     2941 gagccgcact gactttggct tcgatacagc tgggcgaccg tgcgcccgcc gtgtcggcga
     3001 cgtcggaaat gttgcgcatc ccttgctccc ggcgaacggc catcctggcc tcgcgcggtc
     3061 cgtcagattg caaataccgc gccaacggct cgcgcgcggt ctttgccggt cagcgatcga
     3121 gcgacggcag cagcgcttcc ctcgtgggtg gcaagtgctg gtcgacggcg ttcagcatga
     3181 tgccgacgcg cgtgtagtcg cccatgatcg acatgaagtc gacggtgcct tggcgcccca
     3241 gcagcgcgac catcttcgcc cacagctcgc tgctgacctt gtgctcgcgc agcaaggtgc
     3301 ggccgaaggt gatcatcgtc gcgtctttgt cggcgagccc cgcgacgggt cgatcgtatt
     3361 tcacgacgtc gatgacgttt tgctcgagcc cctgccggcg accggcgggc tcgtgcgccg
     3421 accactcgta ttgctggtcg atctcgcgcg cggcgatcag caccgcgagc tgaaattgcc
     3481 gcgcgccgat cgggctgttc tcgccgctcg gaatgccgag gctgcgatca cgcgggctgt
     3541 agacggtgac gtgcgtgggg ccggggcccg gcgtgaacgg tcgggcggcg agcagcttgc
     3601 gggcctcctc gtcgagatcc tcgggcgtca ccggcggcaa gcgcgacaac gtgacggggt
     3661 ggatgtcggg cggcaacgcg gtttgcggcg cagcgatcgt cggcaacgcg gcaagcaaca
     3721 aggcggatat tcgcatgttc attgatgacg cctcccccag gttgggaatt caccctagca
     3781 aggcgccagt cagaactcca ccgtacggtg gcagcagcta gaatcgggcg ctcgccaaac
     3841 cggccggggt gcggatcatg ctgcggttac tacgcccaac gctgctcgcg ctcgtcgccg
     3901 ctgccgcgct gcccgccatc gcgcaagacg acgtcgacgt cttcggcctc gaggccggtg
     3961 agcactcggt cggctttcaa ctgctcgagg acgtcgacgc gtcacgcgtc gtgacgggcg
     4021 gtgtccgcgg tgccacacat ccacgcccga ttcgtactta tctgtggtat ccagccgagg
     4081 ctccgcgtcg cgcgcaaccc
//
LOCUS       DATLEE010000762         3212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10261584, whole genome shotgun sequence.
ACCESSION   DATLEE010000762 DATLEE010000000
VERSION     DATLEE010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10261584"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /gene="dnaX"
                     /locus_tag="VM692_16780"
     CDS             complement(<1..1155)
                     /gene="dnaX"
                     /locus_tag="VM692_16780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HUQ53887.1"
                     /translation="MTYTVLARKWRPRRFEELVGQPHVVQALKNSLADNRLHHAYLFT
                     GTRGIGKTTVARVLAKALNCETGIVAEPCGVCAACTAIDAGRFVDLIEVDAASRTKVD
                     DTRELLDNVQYAPTRGRFKVYLIDEVHMLSNHSFNAILKTLEEPPPHVKFLLATTDPQ
                     KLPVTVLSRCLQFNLKRFTVAQIQAQLEKICTAEGIPFDAAGLKAIARAAAGSMRDGL
                     SLLDQSIAFGGGKIEGSMVAAMLGSIDRGHVLRLLAALAKQDGTALLAEVEKLDESAP
                     DYGAALDELLEALQRIAVLQLVGGRTDDEDFAAVAPFVEQFSAEDAQLFYQIALHGRR
                     DLPLCREPRMGFEMTLLRMLAFRPAAEEGAPQRAVRTTPSAPPPRADAAPA"
     gene            complement(1185..1281)
                     /gene="ffs"
                     /locus_tag="VM692_16785"
     ncRNA           complement(1185..1281)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VM692_16785"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1399..1491
                     /locus_tag="VM692_16790"
     tRNA            1399..1491
                     /locus_tag="VM692_16790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1433..1435,aa:Ser,seq:gga)
     gene            1565..2521
                     /locus_tag="VM692_16795"
     CDS             1565..2521
                     /locus_tag="VM692_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HUQ53888.1"
                     /translation="MADKKIIAVLGSTGSQGGGLVRAILADSAGGFAARAVTRDTSKD
                     KANALATAGAQVVKGDLDDVESLKKAFAGAHGVYAVTNFWEHFSGEKEKAQAKNVADA
                     AKAAGVKHVVWSTLEDIRQFMKPDDKRMPILQEKYRVPHFDAKAEANASFSGLPTTFL
                     VTSFYWDNLYMFGLAPKKGDDGKLAWTFPMGTSKMAGIAAEDIGKVAYGIFKAGDQYI
                     GKTVGVMGEALTIEQMGQTLSKVLGVGPVQYNAVEADVYRSFGFPGADEMGNMFQAYR
                     DFDKQMLANRSVDTARKLNPALQSFEQFVTKQKAAIKTAMGI"
     gene            complement(2585..>3212)
                     /locus_tag="VM692_16800"
     CDS             complement(2585..>3212)
                     /locus_tag="VM692_16800"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ53889.1"
                     /translation="NRSYIAGGVRAATGLRLVTFYFADEAALAARFVQHGLPAPQFTD
                     MRAEKRRTALVTDPDGQEVELEIVRGATAEQLARIEVGLTVADLERSRAFYRSFVGLE
                     ELPPVEDERFGTRKYSFRHGATTISLRGFGKDLPADTGSGGIQYVVSDVEFVDALAKA
                     RHITIDQPLSGLRGFDLRTVWLGDPDGITNYFAETGASRGARATAPAQ"
BASE COUNT          580 a         1052 c         1046 g          534 t
ORIGIN      
        1 cgccggcgcc gcgtccgcac gaggcggcgg ggccgacggc gtcgtgcgca cggcacgctg
       61 cggtgcgccc tcttcggccg ccggccgaaa agccaacatg cgcagcagcg tcatctcgaa
      121 gcccatgcgc ggctcccggc acaacggcag atccctgcgg ccgtgtaacg cgatctgata
      181 gaacagctgc gcatcctccg cggagaattg ctcgacgaac ggcgcaacgg ccgcgaaatc
      241 ttcgtcgtcg gttcgcccgc cgacgagctg cagcaccgcg atgcgttgca gcgcctcgag
      301 cagctcgtca agtgcggcgc catagtcggg ggcgctctcg tcgagcttct ccacctcggc
      361 cagcagcgcg gtgccgtcct gcttggccaa cgccgcgagc agccgcagca catggccgcg
      421 atcgatgctg cccagcatcg cggcgaccat cgaaccctcg atcttgccgc cgccgaacgc
      481 gatgctctga tcgagcaggc tcagcccgtc gcgcatgctg ccggcggccg cgcgtgcgat
      541 ggctttcaga ccggccgcgt cgaacgggat gccctcggcc gtgcagatct tctcgagctg
      601 cgcttggatc tgcgcgacgg tgaaacgctt caagttgaac tgcaggcaac gcgatagcac
      661 ggtcacgggc agcttctgcg gatccgtcgt cgcgagcaga aacttgacgt gcggcggcgg
      721 ctcttcgagc gtcttcaaga tcgcgttgaa cgaatggttc gacagcatgt ggacctcgtc
      781 aatgaggtac accttgaacc gcccgcgcgt cggtgcgtat tgcacgttgt cgagcagctc
      841 gcgcgtgtcg tcgactttcg tgcgcgacgc cgcgtcgacc tcgatgagat cgacgaaccg
      901 gcccgcatcg atggccgtgc acgcagcgca tactccgcac ggctcggcga cgatgcccgt
      961 ctcgcagttc agcgctttcg cgagcacccg cgcgacggtg gtcttgccga tcccgcgtgt
     1021 gcccgtgaac aggtacgcgt gatggagccg gttgtcggcg agcgaattct tgagcgcctg
     1081 cactacgtgc ggctgaccga cgagctcctc gaaccgccgc ggtcgccatt tacgcgccag
     1141 aaccgtgtac gtcacgccgt tccctgtcca agaaaaaagg aggtccgcgc cagcccgacc
     1201 ccggcaccca accgggctgc taccgctgct cccttccggg cctgacgggg tttacagctg
     1261 gttgtcgcgg gggaaccgac gcggaccaag cggcatttta ccagcatggc ggagcgcacg
     1321 gcgccggcgt ttgaatcggc tatgagttcg ggctacgctg ccgccccccg gcacctcgca
     1381 gccggaagct cgaactccgg agaggtggcc gagcggttga aggcgcacgc ctggaaagtg
     1441 tgtatacgcc caaaaggtgt atcgagggtt cgaatccctc cctctccgcc acgatctact
     1501 taaagcacgc aaagccgttt atgcttcgcg tcttccccat tgaacgtctc accaaggaat
     1561 tgacatggcc gacaagaaaa tcattgcagt actgggctcg acgggttcgc aaggcggcgg
     1621 cttggtccgc gccattttgg cggattccgc aggcggcttc gccgcgcgcg ccgttacgcg
     1681 cgacacgagc aaggacaaag cgaacgcgct ggctaccgcg ggtgcgcagg tggtcaaggg
     1741 cgacctcgac gacgtcgaga gcttgaagaa agccttcgcg ggcgcgcatg gcgtctatgc
     1801 ggtcacgaat ttctgggagc acttctcggg cgagaaggaa aaagcgcagg cgaagaatgt
     1861 cgccgacgcg gcgaaggcgg ccggtgtcaa gcacgtcgtc tggtcgacgc tcgaagacat
     1921 ccgccagttc atgaagcccg acgacaagcg catgccgatc ctgcaggaga agtatcgcgt
     1981 gccgcacttc gacgcgaaag ccgaggcgaa cgcttcgttc agcggcctgc cgacgacgtt
     2041 cctcgtgacg tcgttctatt gggacaatct gtacatgttc ggcctcgccc cgaagaaagg
     2101 cgacgacggc aagctcgcgt ggacgttccc gatgggcaca tcgaagatgg ccggcatcgc
     2161 ggccgaagac atcggcaagg tggcctacgg catcttcaag gccggcgatc aatacatcgg
     2221 caagacggtc ggcgtcatgg gcgaggcgct gacgatcgag cagatgggac agactctgag
     2281 caaggtgctc ggcgtcggtc ccgtccagta caacgccgtg gaagccgacg tctatcgtag
     2341 cttcggcttc ccgggcgccg acgagatggg caacatgttc caggcctatc gcgacttcga
     2401 caagcagatg ctggcgaatc gcagcgtcga cacggctcgt aagctgaacc ctgccctgca
     2461 gagcttcgag cagttcgtca cgaaacagaa ggcggcgatc aagacggcga tgggcatctg
     2521 acccagcgct aggttccgtt ccaaaaagcg gcgagctcgc gagagctcgc cgcttttctt
     2581 ttgactactg cgccggcgcc gtagcgcgtg cgccgcggct ggcgccggtt tccgcgaagt
     2641 aattcgtgat gccgtctgga tcgccgagcc agacggtccg cagatcgaag ccgcgcaagc
     2701 ccgagagcgg ctgatcgatc gtgatgtgac gcgccttcgc gagggcgtcg acgaactcga
     2761 cgtccgagac cacgtactga attccgccgc taccggtgtc ggccggcaaa tccttgccga
     2821 atccgcgcag gctaatcgtg gttgcgccgt ggcggaacga gtacttgcgc gtgccgaagc
     2881 gctcgtcttc gacgggcggc agctcctcga ggcccacgaa actacggtag aacgcgcggc
     2941 tgcgctcgag atctgcgacc gtgagcccga cctcgatgcg agcgagctgc tcggccgtcg
     3001 cgccacgaac gatttccagc tcgacctcct ggccgtcggg gtccgtgacg agcgccgtgc
     3061 ggcgcttctc cgcgcgcatg tccgtgaact gcggcgccgg cagcccgtgc tgcacgaagc
     3121 gggccgcgag cgccgcctcg tcggcgaaat agaaggtcac gagccgcagc cccgtcgccg
     3181 cgcgtacgcc gcccgcgatg taactgcggt tg
//
LOCUS       DATLEE010000763         4590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10262904, whole genome shotgun sequence.
ACCESSION   DATLEE010000763 DATLEE010000000
VERSION     DATLEE010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10262904"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VM692_16805"
     CDS             <1..559
                     /locus_tag="VM692_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53890.1"
                     /translation="NRTNDMLYLRSNFEREVLEVPFAIRPGTIIPVGDYSFDDHGLEW
                     GFSAHRRFAGRIAYTNGEFYNGTRERTFGSFTWAPSPRYRTSTGFNIQYIDLPVTNGK
                     FATRIITQTFDIVFSSKLSWTNLIQYDNVSEVMGLNLRLNWIPEAGRELYFVINHNLQ
                     DFDRDNRFHSVTSDVVAKASYTFRF"
     gene            complement(541..2409)
                     /locus_tag="VM692_16810"
     CDS             complement(541..2409)
                     /locus_tag="VM692_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HUQ53891.1"
                     /translation="MSTSQRSSSKPLEDYGLIGNMISAALVARDGSIDWLCLPHFDSA
                     ACFAALLGGPEHGRWLLAPADRHARSSRRYLPDTAILETRFENERGAVTVTDFMPLTE
                     DEDKVDVVRIVKGIRGEMPMRMELVLRFDYGRTVPWVRKRDYGLSAVAGPDALELHTD
                     IALEGRDFKTLAEFTVREGESVPFTLSYHRSHRTPHFVPDRTETMEATATWWREWSKH
                     CRFVCDNEKWRDAVKRSLITLKLLTYAPTGGIVAAPTTSLPESLGGPRNWDYRYCWIR
                     DSTLTLYAFINAGYREEAVAWRAWLLRAAAGLPEQLHVLYGIAGERCLPELIVDWLPG
                     YENSRPVRVGNAAIMQSQLDVYGEIMDAMHAARQAELAPSSDSWRLQKALLKHLERVW
                     QQPDHGIWEVRGPPQQFTHSRLMCWVAFDRAAKSCEELALDESPARWQALRDHIKADI
                     LANGFDERRNSFVQYYGGNSLDASLLLIPLVGFLPPDDSRVTGTIDAIARELAPNGFV
                     RRYATQHVDDGVGGPEGAFLACSFWLADAYAVTGRTREAEELFERLLAVRNDLGLLAE
                     EYEPASRRLLGNFPQAFSHIGLVNTAFNLVQAHGPAKQRAERTGPPSENRAQKRNV"
     gene            2692..>4590
                     /locus_tag="VM692_16815"
     CDS             2692..>4590
                     /locus_tag="VM692_16815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUQ53892.1"
                     /translation="MALNGKQSGTRVARRLFTSLALASLVPIGTSALFTVVETHSTLR
                     ARAESELTQASQDLGERLVERLRIADDMLAALSAHEPFDALMFDAVEVRTEQAGHAVE
                     GTLAAVADIDSDVSGSSRLLVDTRGSEPRILLARANATGLSLARLAPAYLWKTDRSLP
                     LGTSFCVFAPGVVEPVHCNERLSDNALRSLAALRAAVSSGIAGLHDGDLPLTAAYSQL
                     PLPAEFAAVPWSIVASRPAPTAVDSLRSLGNAYSLAFVISCLAVLLLAIVQTRRILYP
                     LGRLVEGTRKIAAREFTTRFGLHGDDEFRELAHAMNAMTEQLGRQFETLTALRDIDGL
                     ILSSEQTDGILDAVLARVATIVQGCDIAVLLIDPDNPEFARLYRRVVLAHTVQRSSIR
                     CPISQELRRWLAQRDGGSLDQGADVRKHLAAFGAIDDRSRVFVKPLVHGEQLRGAMFA
                     LFESGAVAEDADLDSLRELANRIAVATSAAEREQQLFRRAHFDALTGLPNRQLCHDRL
                     GQALAVARRQDHKLAVLFIDLDGFKHVNDSLGHAAGDQLLREASVRLRSAVRATDTVA
                     RLGGDEYVVILPSVHGPLEVEVIVGSIMEALKWPFLVNGEQCLIGASIGVTMFPDDGS
                     TAEELLRKA"
BASE COUNT          779 a         1514 c         1501 g          796 t
ORIGIN      
        1 caaccgcacg aacgacatgc tgtacctgcg ctcgaacttc gagagggaag tgctcgaggt
       61 gccgttcgcg atccgcccgg gcacgatcat ccccgtcggc gactattcgt tcgacgacca
      121 cggcttggag tggggattct cggcgcaccg acggtttgcg ggccgcatcg cctatacgaa
      181 cggcgagttc tacaacggca cgcgcgagcg cacgtttggc agcttcacgt gggcgccgtc
      241 gccacggtac cgcacgagca ccggcttcaa cattcaatac atcgatctgc ccgtgaccaa
      301 cggcaagttc gcgacgcgca tcatcacgca gacattcgac atcgtgttct cgtcgaagct
      361 gtcgtggacg aacttgatcc agtacgacaa cgtcagcgag gtcatgggcc tgaacctgcg
      421 cttgaactgg atccccgaag ccgggcgcga gctctacttc gtgatcaatc acaacctgca
      481 ggacttcgac cgcgacaacc gcttccactc ggtcacgagc gacgtcgtcg cgaaggcgag
      541 ctacacgttc cgcttctgag cgcggttctc gctcggcggc ccggtacgtt cggctcgctg
      601 cttcgccggc ccgtgggcct gcacgagatt gaacgccgta ttcacgaggc cgatgtgcga
      661 aaacgcttgc gggaagttgc cgagcaagcg gcgcgacgcg ggctcgtatt cctcggcgag
      721 caaacccaag tcgttgcgca cggcgagcaa tcgctcgaat agctcctcgg cctcgcgcgt
      781 gcggcccgtg acggcgtagg cgtctgcgag ccagaacgag cacgcgagaa acgcgccctc
      841 tggaccgccg acgccgtcgt cgacgtgctg ggtcgcgtag cggcgcacga agccgttggg
      901 cgcgagctcg cgcgcgatgg cgtcgatcgt gccggtcacg cgcgagtcgt cgggcggcag
      961 aaagccgacg agcggtatca gcagcaacga cgcgtcgagc gagttgccgc cgtaatattg
     1021 gacgaacgag ttgcgccgct cgtcgaagcc attcgcaagg atgtcggctt tgatgtgatc
     1081 gcgtagcgct tgccagcgcg cgggcgactc gtcgagcgcc aactcctcgc agcttttcgc
     1141 cgcgcggtcg aaggcgaccc agcacatgag gcgagagtgg gtgaactgct gcggcgggcc
     1201 gcgcacttcc caaatcccat ggtcgggctg ctgccagacg cgctcgaggt gcttcagcag
     1261 cgctttttgc agccgccacg aatcggacga cggcgccaac tcggcttggc gagccgcatg
     1321 catcgcatcc atgatctcgc cgtagacgtc gagttggctc tgcatgattg cggcgttgcc
     1381 gacgcgcacc ggccggctgt tctcgtagcc gggaagccag tcgacgatga gctcgggcag
     1441 gcagcgctcc ccggcgatgc cgtagagaac gtgcagctgc tcgggcagtc cggccgcggc
     1501 tcgcaatagc caagcgcgcc acgcgacggc ttcctcgcga tagcccgcgt tgatgaacgc
     1561 atagagtgtc aacgtggaat cgcggatcca gcagtagcgg tagtcccagt tgcgcggccc
     1621 gcccagcgac tccggcagcg acgtcgtcgg cgcggcgacg atgccgcccg tcggcgcgta
     1681 cgtcagcagc ttcagcgtga tcagcgagcg cttcacggcg tcgcgccact tctcgttgtc
     1741 gcatacgaag cggcaatgct tggaccattc gcgccaccag gtcgccgtcg cttccatcgt
     1801 ctcggttcgg tccggaacga agtgcggcgt gcggtgcgaa cggtgatacg acaacgtgaa
     1861 cggcacgctc tcgccttcgc gcaccgtgaa ctcggccagc gttttgaagt cgcggccctc
     1921 gagcgcgatg tcggtgtgca gctcgagcgc gtcggggccg gcgaccgccg atagcccgta
     1981 gtcgcgcttt cgcacccagg gcacggttcg gccatagtca aaccgcaaga cgagctccat
     2041 gcgcatcggc atctcgccac gaatgccctt cacgatgcgg acgacgtcga ctttgtcctc
     2101 gtcttccgtc aacggcatga agtccgtcac cgtgacggcg ccgcgctcgt tctcgaagcg
     2161 cgtctcgagg atagcggtgt ccggcaggta acgccgcgac gagcgggcgt gacggtccgc
     2221 cggtgcaagg agccaacggc cgtgctcggg cccgcccagc agtgccgcga aacaggcggc
     2281 cgaatcgaag tgcggcaggc acagccagtc gatcgagccg tcgcgcgcga cgagcgcggc
     2341 ggagatcatg ttgccgatga ggccgtagtc ctcgagcggc ttcgaggaag atcgctggct
     2401 cgttgacatc actgcaccac aagacggttg cagccagtcg atttgcaagg cgcatgccag
     2461 tggcagcggg gttcggccgc cgtgcacggc aagctcagcg tccgaggtcg acgtcgaggg
     2521 ccgcacagct tcgcgaagcc gcggagggac gtgctgctca gtgcggcggc tagccggttt
     2581 ccataatgcg ggctccgaag ggaagccgtg aaggcggcca cagcgcccgc gacgcccagc
     2641 gcgtagcctc cactcaccgt cgcctaacct cctgcgacgg aacggcgagc gatggcgctg
     2701 aatggcaaac agtccggtac ccgggtcgcg cgccggctct tcacgtcgct tgcgctcgcg
     2761 agcctcgttc cgatcggcac gtcagccctc ttcacggtcg tcgagacgca tagcaccctg
     2821 cgggcccgcg ccgaaagcga gctgacacaa gctagccagg acctcggcga gcggctcgtc
     2881 gagcggttgc gcatcgccga tgacatgctg gcagcgttat ccgcgcatga gccgttcgac
     2941 gcgctcatgt tcgatgccgt ggaggttcga accgagcagg ccgggcacgc ggtcgagggg
     3001 accctggccg ctgtggccga tatcgacagt gacgtgtcgg gcagcagtcg cctcctggtg
     3061 gacacgcgcg gaagcgagcc tcggatcctg ctcgcccgcg cgaacgcgac tgggctgtcc
     3121 cttgcaaggt tggctccggc ctacctctgg aaaacggatc gttcgttgcc gctcgggacg
     3181 agtttctgcg tgttcgcgcc cggcgtcgtc gagcccgttc actgcaacga gcgtttgtcg
     3241 gataacgcgc tgcgctcatt ggccgccttg cgcgccgcgg tatcgagtgg catcgcgggc
     3301 ttgcacgacg gcgacctgcc gttaacagcc gcctattcgc aattgccgtt gcctgcggaa
     3361 ttcgcggctg tcccttggtc catcgtcgct agccgaccgg cgccaacggc cgtcgactcc
     3421 ctgagatcgc tcggcaacgc gtattctctg gccttcgtga tcagctgtct cgcggtcttg
     3481 ctgctcgcga tcgtgcaaac gcgccggatc ttgtacccgc ttggccggct cgtagagggc
     3541 acgcgcaaaa tcgcagcacg tgagttcacg acgcgcttcg gcctacacgg cgatgacgaa
     3601 tttcgcgagc tcgcccacgc catgaatgcg atgaccgagc aactcgggcg gcagttcgag
     3661 acgctaaccg cattgcggga catcgacggg ctgatcctgt cttcggagca gaccgacggt
     3721 attctcgacg cggtgctcgc gcgcgtcgcg acgattgtcc aaggatgcga catcgccgtg
     3781 ttgctcatcg acccggacaa tcctgagttt gcgcgcttgt accgacgagt cgttcttgcc
     3841 cacacggtgc agcgctcgtc gatccgctgc ccaatttcgc aggagctccg ccgctggctc
     3901 gcccaacgcg acggcggcag cctcgaccaa ggtgccgacg tgcgtaaaca tctcgctgcc
     3961 tttggcgcaa tcgacgatcg ctcccgcgtg ttcgtgaagc cgctcgtcca tggcgagcag
     4021 ctgcgcggag cgatgttcgc gctgttcgaa tccggcgccg tcgccgaaga cgcggacctg
     4081 gattcgctgc gcgagctcgc gaatcgcatc gcggtcgcga cgtccgcggc cgagcgcgag
     4141 caacaactat tccgtcgcgc gcacttcgac gcactgaccg ggctaccgaa cagacagctg
     4201 tgccacgacc gtttgggtca ggcactggcc gtcgcacggc gtcaggacca caagctcgcc
     4261 gtgctgttca tcgacctcga cggcttcaag cacgtcaacg actcgctcgg tcatgcggca
     4321 ggcgaccagc tcttgcgcga ggcatcggta aggctgcgct ctgccgtgcg cgcgaccgat
     4381 acggtggcaa ggctgggcgg cgacgagtac gtcgtcattc tgccgagcgt gcacgggccg
     4441 ctcgaggtcg aagtcatcgt cggcagcatc atggaggctt tgaagtggcc gtttctcgtc
     4501 aacggtgaac agtgcctcat cggcgcgagc atcggcgtaa cgatgttccc cgacgatggc
     4561 agtaccgccg aggagctgtt gcgcaaggcc
//
LOCUS       DATLEE010000764         2110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8053553, whole genome shotgun sequence.
ACCESSION   DATLEE010000764 DATLEE010000000
VERSION     DATLEE010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8053553"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /gene="aroE"
                     /locus_tag="VM692_16820"
     CDS             <1..691
                     /gene="aroE"
                     /locus_tag="VM692_16820"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HUQ53893.1"
                     /translation="AAFETAVRSFGAAGGKGVNVTVPHKEAAFALCDDATAAARAARA
                     VNTISIAEGKLHGDNTDGVGFIRDVMVNQRQTLAGRRILLLGAGGAARGIVGPLLAAQ
                     PAELTIANRSKDRADQVVAQFGAAPLAARSFAELEGMPPFDVIVNATSAGLKGETPPF
                     PGSLVGPRSFCYDLVYGTSATPFVMWARSHGAARAVQGWGMLVEQAAEAFTIWRGVRP
                     DTKALLKQLAR"
     gene            complement(710..1984)
                     /locus_tag="VM692_16825"
     CDS             complement(710..1984)
                     /locus_tag="VM692_16825"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HUQ53894.1"
                     /translation="MDDQDARPYAAFRLSLEQQQKRAKDLLKAAKAADPGALRRLQSV
                     GVASAGGVKLAQAQHCIARELRFASWADLKRHVGAMARSRAVLGAGVLDAGCRTMHIR
                     CGHDIEHALRDAGFRGDFNAHTNPYLQGPVTDATDWLEQRARYIAEAAGPYLGVEYAA
                     VLEGGRDEERRLVAASRDYERVVLWLEHDRYDQLALLRSLASFAEHGAPPRLELIGPS
                     DFPGATRFVGLGQLPPEALCLLWQRRAPVSTEQIAFGARAWSAFRAADPSPLAELVRR
                     GTPLLPDLAAALRRHLQELPSVRNGLGLTHELLLQALADHGTQPAGRLVGHVMHSDDP
                     LPGLGDIGYDQALREMAVGSEPLVRRAGGHPPPAWSRDEVALTSSARSVLHGSRDWLQ
                     STVPERWVGGVRIVGGQRNWRWDDAARSVVLT"
BASE COUNT          337 a          745 c          716 g          312 t
ORIGIN      
        1 cgccgctttc gagacggccg tgcgaagctt cggtgccgcc ggcggcaagg gcgtcaacgt
       61 gacggtgccg cacaaggagg ccgcatttgc gttgtgcgac gacgcgaccg ccgccgcacg
      121 cgcggcgcgc gccgtcaaca cgatcagcat cgccgaaggc aagctgcacg gcgacaacac
      181 ggacggcgtc ggcttcattc gcgacgtcat ggtcaaccag cggcaaacgc tcgcgggccg
      241 caggatcctc ctgctcggcg caggcggcgc ggctcgcggt atcgtcgggc cgctgctcgc
      301 ggcgcaaccc gccgagctga cgatcgccaa tcgcagcaaa gatcgcgcgg accaagtcgt
      361 cgcgcagttc ggcgccgcgc cccttgccgc gcgctcattc gccgagctcg aaggcatgcc
      421 gccgttcgac gtcatcgtca atgcaacctc ggccggcctc aagggcgaga cgccgccgtt
      481 tccgggctcg ctggtcggcc cgcggagttt ctgctacgac ctcgtctacg gcacgagcgc
      541 gactccgttc gtcatgtggg cgagaagtca tggcgccgcg cgcgccgtgc aaggttgggg
      601 aatgctcgtc gagcaggccg ccgaggcgtt tacgatttgg cgcggcgtgc ggccagacac
      661 caaagcgctg ttgaagcagc tcgcccgcta gcgccggccg cacgggcgat cacgtcagga
      721 cgacgctgcg agccgcgtcg tcccagcgcc agttgcgctg tccgccgacg atgcgaacgc
      781 cgccgaccca acgttccggc acggtcgact gcaaccaatc gcgcgagccg tgcaataccg
      841 agcgggccga gctcgtgaga gcgacttcgt cgcggctcca cgccggcggc gggtgtccgc
      901 ccgcacgccg caccagcggc tcggaaccga cggccatctc gcgcagagcc tggtcgtagc
      961 cgatgtcgcc gagccccggc agcggatcgt cgctatgcat gacgtggccg acgagccgcc
     1021 ccgcgggttg cgtcccgtga tccgcgagcg cctgtaacag cagctcatgc gtcagaccga
     1081 gcccgttgcg caccgacggc aactcttgca gatgtcggcg caacgcagca gcgagatcgg
     1141 gcagcaatgg cgtgccgcga cggacgagct ccgcgagcgg gctcggatcg gctgcgcgga
     1201 atgcgctcca agccctcgcg ccgaaggcga tttgctccgt gctgacgggt gcgcgccgct
     1261 gccacagtaa acacagtgcc tccggcggta actgcccgag ccccacgaac cgcgttgcgc
     1321 cggggaaatc gctgggtccg atcagctcga gccgcggtgg agcaccgtgc tcggcgaacg
     1381 aggcgaggct tcgcagcaac gcgagctggt cgtagcgatc gtgctcgagc cacagcacga
     1441 cgcgctcgta gtcgcggctc gcggcaacga gccgccgctc ctcgtcacgg ccgccctcga
     1501 gcacggcggc atactcgacg ccgaggtatg gccctgcggc ttcggcgatg tagcgcgcgc
     1561 gctgctcgag ccaatcggtt gcgtccgtga cgggaccttg cagatacggg ttcgtgtgag
     1621 cgttgaagtc gccacggaac ccggcatccc gcagcgcgtg ctcgatgtcg tgcccgcagc
     1681 ggatgtgcat cgtgcgacag ccggcgtcga ggacgcctgc gccgagcacg gctcgcgacc
     1741 gcgccattgc accgacatga cgcttcaaat cggcccagct cgcgaagcgc agctcacgcg
     1801 cgatgcagtg ttgcgcctgc gcgagcttga cgccgccggc gctcgcgact cccacgcttt
     1861 gcagccgtcg cagcgcgccg ggatcggccg cctttgctgc cttcagcaag tccttcgcgc
     1921 gtttttgctg ctgctcgaga ctgaggcgga aggcggcgta aggccgcgcg tcttgatcgt
     1981 ccacgaacgt ctcctttcag aacgcctgcg cccgcagtca gacggaaaag agacgaactc
     2041 gaatcctgtc tcagcgaaac agtcgtgggc gcagcccttt ccgcggacgt ggatgctccg
     2101 accgagcgcg
//
LOCUS       DATLEE010000765         2304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8055870, whole genome shotgun sequence.
ACCESSION   DATLEE010000765 DATLEE010000000
VERSION     DATLEE010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8055870"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..824
                     /locus_tag="VM692_16830"
     CDS             222..824
                     /locus_tag="VM692_16830"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HUQ53895.1"
                     /translation="MRPETEARIRNVKKFAKNGWQFCAFAGFMLGIYLLGAWVTIVFR
                     FAGTRLNVGTFYSVNADLLTAPSSRIWSFVVVTVVAGLSAWMLLHLYRLFKHLEAGSI
                     YTQQNVHHLRQVGWLSMALAVIQLLLPLLAFVLAELNLVDATLVPRTETANGAPIWLG
                     QSLSGVITASLILLASWIMDVGRQISDDADAMRREADLVI"
     gene            838..1086
                     /locus_tag="VM692_16835"
     CDS             838..1086
                     /locus_tag="VM692_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUQ53896.1"
                     /translation="MPIVVNVDVMLARRKMRLNELAERVGITPQNLSVLKTGRARAIR
                     FSTLEKLCEVLECQPGDLFAFEGPASSATESGEPDAAA"
     gene            complement(1083..1772)
                     /locus_tag="VM692_16840"
     CDS             complement(1083..1772)
                     /locus_tag="VM692_16840"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUQ53897.1"
                     /translation="MSERKVKLLALDLDGTLVDSAPDIAHCLGSALEAIGYAAPGEAR
                     TRVWIGDGLETLIARAIAHGTGGQVESTIASEARHQAALTAFLSCYADNLFTRSRLYP
                     GVLPVLDELRERGLRLCCITNKRFAFSQELLVQAGIRDRFELLLGGDSLPEKKPSPLP
                     LTTAAESLGVEPRTAILVGDSHQDLRAARAAGYGFVLADYGYGKIDETELGTSQRIRT
                     FAELPAVLGGS"
     gene            complement(1769..>2304)
                     /locus_tag="VM692_16845"
     CDS             complement(1769..>2304)
                     /locus_tag="VM692_16845"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HUQ53898.1"
                     /translation="FFVIKACGAAAAEGRDLDSLLALADKVNNNVRTMGVALTSCIPP
                     AKGTTIFELGDDEMEVGVGIHGEPGRRRDKVKSGNEIVDELFEAVSQDIPFNSGDRVA
                     IMINGLGGTPISELYLLYARAHELAEKAGYTVVRNYVGNYCTSLEMAGASLTLLKLDK
                     EIEELLAAPADIAVRVF"
BASE COUNT          393 a          740 c          750 g          421 t
ORIGIN      
        1 aggacggcgc ggcgaagaga acctcaccag cagccgcgaa tcaccggcgc gccacgagtg
       61 tccgcggcga tgcgcgcggc tacttcttcg ccgagtgttg atacggtcgt agctcaatga
      121 acggcatata atgatcgttt atcgataaaa aagtgttgac actcgggatg ggtgtccgta
      181 gtctctgcgg ccatcgctgc gtagccacgg agagcccgac aatgagaccc gaaaccgaag
      241 cccggatccg caacgtcaag aagttcgcaa agaacgggtg gcagttctgc gcgttcgccg
      301 gcttcatgct cggcatctac ctgctcggtg cttgggtgac gatcgtcttc cgtttcgcgg
      361 gcacacggct caatgtcggc accttctaca gcgtcaatgc cgatctgttg acggctccgt
      421 ccagcaggat ctggtcgttc gtcgtggtga cggtcgtggc aggcctgtcc gcctggatgc
      481 tgctgcacct gtatcgcctc ttcaagcacc tcgaggcggg atcgatctac acgcaacaga
      541 acgtccacca cctgcggcag gtcggctggc tgtcgatggc gctcgccgtg attcaactgc
      601 tgttgccgtt gctggcgttc gtgctcgcgg agctgaatct cgtcgacgct acgctagtcc
      661 cgcgcacgga gaccgctaac ggagcgccga tatggttggg tcaatcgttg tccggtgtca
      721 tcacggcgtc gctgatcctg ctcgcctcgt ggatcatgga tgtcggccgg cagatcagcg
      781 acgacgcgga tgcgatgcgc cgagaagccg atctcgtcat ctagcgcgga gcccaccatg
      841 ccgatcgtcg tcaacgtcga cgtgatgctc gcgcggcgca agatgcgcct caacgagctg
      901 gccgagcgcg tcggaatcac gccgcagaac ttgtccgtgc tgaagacggg cagggcgcgc
      961 gcgatccgct tctcgacgct cgagaagctt tgcgaagtgc tcgagtgcca gcccggagat
     1021 ctgttcgcct tcgaagggcc cgcgtcgagc gccacagaga gcggcgagcc cgacgcggcc
     1081 gcttagctgc cgccgagcac ggccggcagc tcggcgaagg tgcggatgcg ctggcttgtg
     1141 ccgagctccg tctcgtcgat tttgccgtag ccgtagtcgg cgagcacgaa gccgtagccg
     1201 gcggcgcggg ccgcacgcag atcctgatgc gagtcgccga cgagaatcgc cgtgcgcggt
     1261 tcgacgccga gcgactcggc cgccgtcgtc agcggcagcg gactcggttt cttctcgggc
     1321 agactgtcac cgccgagcag cagctcgaac cggtcgcgaa tgccggcttg cacgagcagc
     1381 tcttgagaga acgcgaagcg cttgttcgtg atgcagcaga gcctgagccc gcgctcacgc
     1441 aattcgtcga gcaccggcag gacgcccggg tagaggcgac tgcgcgtgaa cagattgtct
     1501 gcgtagcagc tcaggaaagc ggtgagggcg gcctggtgcc gcgcctcgct cgcgatcgtc
     1561 gactcgacct gcccgccagt gccatgcgca atcgcgcgcg cgatcaacgt ctcgagaccg
     1621 tcgccaatcc acacgcgcgt gcgcgcctcg cccggcgctg catagccgat cgcctctagc
     1681 gcagaaccca agcagtgcgc gatatcgggc gcggaatcga ccagcgtccc gtcgaggtcg
     1741 agggcgagta gcttgacctt gcgctcgctc aaaacactcg aacggcgatg tccgccggtg
     1801 ccgccaacaa ttcctcgatc tctttgtcga gcttcagcag cgtcagcgac gcgccggcca
     1861 tctcgagcga cgtgcaatag ttgccgacgt agttgcgcac cacggtgtag cccgcctttt
     1921 cggccagttc atgggcgcgg gcatagagca ggtacagctc gctgatcggg gtgccgccaa
     1981 gtccgttgat catgatcgcc acccggtcac ccgaattgaa cgggatgtcc tggctcacgg
     2041 cctcgaacag ctcgtcgacg atctcgttgc cgctcttgac cttgtcgcgg cggcggcccg
     2101 gctcgccatg aatgccgacg ccgacttcca tctcgtcgtc gccgagctcg aagatggtgg
     2161 tgcccttggc cggcgggatg caactggtga gcgccacgcc catcgtgcgg acgttgttgt
     2221 tgaccttgtc ggccagcgcg aggaggctgt cgaggtcgcg gccttcggcg gccgcggcgc
     2281 cgcatgcctt gatcacgaag aagt
//
LOCUS       DATLEE010000766         3197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10293412, whole genome shotgun sequence.
ACCESSION   DATLEE010000766 DATLEE010000000
VERSION     DATLEE010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10293412"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VM692_16850"
     CDS             <1..406
                     /locus_tag="VM692_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004882198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53899.1"
                     /translation="HGEVVREVQGAAVQGRNVRFVSLLTMGECWHNNHHAYPGSARLG
                     LAPGEWDPGWWVLCALRRIGLVSGIRLPDDLPYRPELRAPRERVVASQSRAEGAGADQ
                     VKARIASHRPAVPTNATVEATIAAVASASSTP"
     gene            complement(304..1287)
                     /locus_tag="VM692_16855"
     CDS             complement(304..1287)
                     /locus_tag="VM692_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HUQ53900.1"
                     /translation="MTTAVAIKLLAVLLTAALGYGAGRFRLLTAGAAASDAARVLSNA
                     ALYVFVPALLFRTTVRIDYAHMPWRVIAAYFVPVLGVAFVVYAWHHVRSSVAVAAAIP
                     ATRAMAVAFGNSVQLGIPMATALFGEEGLAIHIPLVSLHALTLLTVLTVLVELDLARA
                     AHGRGTGWHSMRRALATTLRNTLLHPVLLPVFAGLAWHLLGLPIPAVFDEVLVLLATA
                     VVPLCLVLIGVSLAQYGIAGHWHGATLATIGKLFVLPAVVLLTARFGFGLTGLPLAVL
                     VMMAALPVGTNALLFAQRYGVLEAEATAAIVASTVAFVGTAGLWLAILALT"
     gene            1394..2527
                     /locus_tag="VM692_16860"
     CDS             1394..2527
                     /locus_tag="VM692_16860"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUQ53901.1"
                     /translation="MPRASLLGVVVLVGAAASAAAVPRQPAFDEAELVSGTALTLEEA
                     ATPFTIPSADDAFELDPDMRDFVAPLLAVHDPRQRLHGLIRSLEEHGMFSLDYAETTR
                     TASATFHGRQGNCLSFTMLFIGLARAAGLTATYQSVEVPPTWSNDGQVVIASHVNAVV
                     RTGRGQETIVDFNIRSARADTRSRRVNDQYALALFYVNLGAEALLREEHAASLAYLRE
                     AAAVYPDIAGLWVNLGVLYARYGRYEHAEAAYLRALQVDEEEESALANLVLVYEALGE
                     QALARQYRDRVQSYRERNPYYHYALASRAYEQQQFADVLLSLRKALRLKHDEHEFYTL
                     RGQALTALGRPRDAEHSFEQAREFEAIEYARRGASVQLEGLDP"
     gene            complement(2535..>3197)
                     /locus_tag="VM692_16865"
     CDS             complement(2535..>3197)
                     /locus_tag="VM692_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53902.1"
                     /translation="GKFLLQIGKSGQSRGSNDTANLGSPADINVDVAAHEVYVADGYR
                     NRRVIVFDSETGAYKRHWGAYGGRPSDDATPAYDPAAAPSRQFGNPVHCVRPTREGLV
                     YVCDRVNDRVQVFRKDGTFVSEAFFERNTRLSGSVSELAFSPDDAQRFVYMVDGVNNE
                     LRIVERSTNNVLARVGRPGRYAGQFHVVHNVAVDSQGNVYTTEVNTGQRVQKFRRLDP
                     QD"
BASE COUNT          556 a         1054 c         1094 g          493 t
ORIGIN      
        1 ccacggcgag gtcgtgcgcg aggtacaagg agccgccgtg caggggcgca acgtgcggtt
       61 cgtgtcgcta ctgacgatgg gtgagtgttg gcacaacaat caccacgcct atccgggctc
      121 tgcacgactc ggccttgcac ccggcgaatg ggatccgggc tggtgggtgc tctgtgcact
      181 gcggcgcatc gggcttgtct cggggatccg gctgccggac gatctgccgt accggcccga
      241 gctgcgcgcg ccacgcgagc gagtcgtcgc gtcgcaaagt cgcgccgagg gcgcgggagc
      301 cgatcaggtc aaagcgagga tcgcaagcca caggcccgcc gtgccgacga acgccaccgt
      361 cgaggcgacg atcgccgccg tcgcttcggc ctcgagcacg ccgtaacgct gcgcaaacaa
      421 cagagcgttg gtgccgaccg gcagcgccgc catcatgacc agcacggcga gcggcaagcc
      481 ggtcaggccg aagccgaatc gcgccgtcag caacacaacc gccggcagca cgaacagctt
      541 gccgatcgtc gcgagcgtag cgccgtgcca gtgacctgcg atgccgtact gcgcgagcga
      601 tacgccgatc agcactaggc agagcggcac gaccgcggtc gctagcaata cgaggacctc
      661 gtcgaagacg gccgggatcg gcagcccgag cagatgccat gcgaggcccg cgaatacggg
      721 cagaagcacc gggtgcagca gcgtgttgcg taacgtggtc gcgagcgctc gccgcatcga
      781 atgccagccc gtgccgcggc cgtgtgccgc gcgcgcgagg tcgagctcga cgagcacagt
      841 cagcacggtg agcagcgtca gcgcatgcag gctcacgagc gggatgtgga tcgccaggcc
      901 ttcctcgccg aacagcgcgg tcgccatcgg aatgccgagc tggaccgagt tgccgaacgc
      961 gacggccatc gcgcgtgtcg cggggatcgc cgccgcgacg gcgacgctgc tgcgcacgtg
     1021 atgccaggcg tagacgacga acgcgacgcc gagcaccggc acgaaatagg ccgcgatgac
     1081 gcgccacggc atgtgcgcgt agtcgatgcg gaccgtcgtg cggaacaaca gcgccgggac
     1141 gaacacgtag agcgcggcat tggacaagac gcgcgcggca tcgctggccg cggcgcccgc
     1201 cgtcagcagg cggaatcggc cggcgccata gccgagcgcc gcggtcagga ggaccgcgag
     1261 cagcttgatc gcaacagccg tcgtcacccg tcgacgctga cggccgctac gcccattttc
     1321 accccgcgat atggcgactt ggccgcgagt ataggcggag tacaatcggg atgtccctct
     1381 tcggatggcg tacatgccgc gggcttcgct gctcggtgtc gtcgttcttg taggggctgc
     1441 agcctcggct gctgcggtcc cgcgccaacc tgcgttcgac gaagccgagc tcgtgtcggg
     1501 cacggcgctc actctcgaag aagcggcaac gccgttcacg atcccgtcgg ccgacgatgc
     1561 gttcgagctc gatcccgata tgcgcgactt cgtcgctccg ttgctggctg ttcacgatcc
     1621 gcgccagcgg ctgcacgggc tcatccgcag cctcgaggaa cacggcatgt tctcgctcga
     1681 ctacgcggag accacgcgga ccgcgagcgc gacctttcac gggcggcagg gcaactgcct
     1741 ctcgttcacg atgctgttca tcggcttggc gcgggcagcc ggcttgactg cgacgtatca
     1801 aagcgtcgag gtgccgccga cgtggtcgaa cgacggccag gtcgtcatcg cgagccatgt
     1861 caacgccgtc gtgcgcacgg gtcgcggcca agagacgatc gtcgacttca atatccgctc
     1921 ggcgcgcgcc gatacgcgca gccgccgcgt caacgatcag tacgcgctcg cgctgttcta
     1981 cgtcaacctg ggcgccgaag cgctgctgcg cgaggagcac gccgcaagcc tggcctacct
     2041 acgcgaggct gccgccgtat accccgacat tgccggcctt tgggtgaatc tcggcgtgct
     2101 gtatgcgcgc tacggacgct acgagcatgc cgaagcggcc tatcttcggg cgcttcaggt
     2161 cgacgaggaa gaggagtcgg cgctcgcgaa cctcgtgctc gtttatgaag cgctcggcga
     2221 gcaagcgctc gcgcgacagt atcgcgaccg cgtgcagagc tatcgcgagc gcaatcctta
     2281 ctatcactac gcgttggcga gcagagccta tgaacagcag cagttcgcgg acgtcttgct
     2341 gtcgctacgc aaggcgctgc ggctcaagca tgacgagcac gagttctaca cgctgcgagg
     2401 tcaggcgttg acggcgctcg gccgccctcg cgacgcggag cacagcttcg agcaagcacg
     2461 cgaattcgag gcgatcgagt acgcgcgccg cggcgcgagc gtgcagttgg aagggctcga
     2521 tccctgaccg cggcctagtc ctggggatcc aggcgccgaa atttctggac gcgctgaccc
     2581 gtgttgacct cggtcgtata aacgttgccc tgcgagtcga cggcgacgtt gtgcacgacg
     2641 tggaattgtc cggcatagcg gcccgggcgg ccgacacgcg cgagcacgtt gttcgtcgac
     2701 cgttcgacga ttcgcagctc gttattgacg ccgtcgacca tatagacgaa ccgctgcgcg
     2761 tcgtcgggcg agaaggcgag ctcggagacc gagccgctga gccgcgtgtt cctctcgaag
     2821 aacgcctcgg agacgaacgt cccgtccttg cggaacactt gcacgcgatc gttgacgcga
     2881 tcgcagacgt agacgaggcc ctcgcgcgtc ggtcgcacgc aatgcacagg gttgccgaac
     2941 tggcgcgacg gagccgcggc cggatcgtag gccggcgtcg cgtcgtcgct ggggcggccg
     3001 ccgtacgcac cccagtgccg cttgtaggcg ccggtctccg agtcgaacac gatgacgcgg
     3061 cggttgcgat aaccgtcggc gacgtaaacc tcatgggccg cgacgtcgac gttgatgtcg
     3121 gccgggctgc cgagattggc ggtgtcgttg ctgccgcggg attgcccgct cttgccgatc
     3181 tgcagcaaaa atttgcc
//
LOCUS       DATLEE010000767         7061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10304170, whole genome shotgun sequence.
ACCESSION   DATLEE010000767 DATLEE010000000
VERSION     DATLEE010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10304170"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VM692_16870"
     CDS             complement(<1..770)
                     /locus_tag="VM692_16870"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HUQ53903.1"
                     /translation="MLDDALLVTYVNSAAENLLGVSSKRTVGRALTEIVHPAQELLVL
                     CQRALATGLSFSLHELAVRVGDREIVLDCRVALLDNEHSLVLEFLDTERDRRVRREAE
                     LVAQQRLSRRIIRQLAHEVKNPLGGLRGAAQLLERQLPDQEQRAYTRVIIEEADRLAA
                     LVDGILRAGGAPKQEEINVHRITEHVAQLIDAEKAAGIELVRDYDPSLPPITVDRNQL
                     IQALLNVGRNAMQALGERGRVIFRTRALTNFTVGGERHR"
     gene            complement(863..1417)
                     /locus_tag="VM692_16875"
     CDS             complement(863..1417)
                     /locus_tag="VM692_16875"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HUQ53904.1"
                     /translation="MRKWLILLSAFSATAVNGAPAWTWTDASGTVHFSDRPVEGARRV
                     ELSGAQGFGSAGAVRSAPRTTASDEQAGSLYQAIDIVSPADQETLWNVGGVLNVQVRF
                     QPALQAGHRFDLAFDGQRRNVNTTTTRVALPDIVRGSHTLQVVVIDAAGAEVTRSAPR
                     TFFVQQTSVLNPNSANGRARAGAN"
     gene            complement(1521..2930)
                     /gene="glnA"
                     /locus_tag="VM692_16880"
     CDS             complement(1521..2930)
                     /gene="glnA"
                     /locus_tag="VM692_16880"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HUQ53905.1"
                     /translation="MSPADVLKLIKEKGVKFVDLRFTDTRGKEQHVTVPSHTIDLGVF
                     EDGKMFDGSSIAGWKGINESDMILGLDPSTAILDPFMEETTLNIRCDIIEPSTMQGYE
                     RDPRSAAKRAEAYMRSTGIADEAFFGPENEFFIFDDVRWHSQMHSGGYTIESYEGAWQ
                     SGKEIEEGNLGHRPGIKGGYFPVPPVDSLQDIRSAMCLALEEMGLVVEVHHHEVATAG
                     QCEIGVKFGSLVKKADDVMILKYVIHNVAHSFGKTATFMPKPLVGDNGSGMHVHQSLA
                     KGGKNLFTGDLYGGLSQTALYYIGGIMKHAHAINALTNASTNSYKRLVKGFEAPTLLA
                     YSARNRSASIRVPYVQNPKARRIEVRFPDSTSNPYLGFTAMLMAGLDGVANKINPGPP
                     SDKDLYDLPPEEEKLIPTVSYSLTQSLEALDKDRAFLTAGGVLNDSTIDAYIGLKEQE
                     IARFSMTPHPVEFEMYYSL"
     gene            3285..4847
                     /locus_tag="VM692_16885"
     CDS             3285..4847
                     /locus_tag="VM692_16885"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HUQ53906.1"
                     /translation="MDAMEKIVSLCKRRGFIFPSSEIYGGLNGAWDYGPLGVELKRNL
                     KQAWWDDMVAHHDETATPAGAPSGFGMVGLDSSLLMNPKVWEASGHVGGFSDPMVDDR
                     ETRARYRADQLAVYALEFIGGDGKLERYDEVLFAALGEVGKTGPAELFEPHRKRIAGL
                     QKSKGGKEARPIPVPGALQDETLRAKVLSPGASAPGTLTAPRAFNLMFKTHVGALEDN
                     SSVTYLRPETAQGIFVNFKNVCDTARVRVPFGIGQVGKAFRNEINPRNFTFRSREFEQ
                     MEIEFFCRPNEAAQWYQYWRERRYRWYLDLGIEQANLHLREHEANELAHYAAGCADVE
                     YDFPFGRSELEGIANRTDFDLRQHMEKSGKDLRYFDDQETNEEARRYLPHVIEPSAGA
                     DRGTLAFLCDAYSEDQVGGEPRTVLKLHPRLAPIKAAVFPLVKKDGMPEKALEIYRTL
                     KKRFSAYYDEKGAIGRRYRRQDEAGTPFCITVDGDTLTAGTVTVRERDSCEQTRVAVG
                     ELADVLGERIGA"
     gene            4852..5403
                     /gene="gmhB"
                     /locus_tag="VM692_16890"
     CDS             4852..5403
                     /gene="gmhB"
                     /locus_tag="VM692_16890"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HUQ53907.1"
                     /translation="MARSAGLVMIDRDGVINEDSGEFIKSVSEWRPIEGSLEAIAALH
                     HAGWRVVVVTNQSGIGRGLYDEAALAAIHRHMRERVRNAGGELAGVYYCPHLPEDGCD
                     CRKPKPGMFRALERELGVSVRGSPYIGDRISDIEAAEGVGARPILVRTGTGAATEGLL
                     AGRNVPVFDDLAAAARDLLDKTR"
     gene            5409..6128
                     /locus_tag="VM692_16895"
     CDS             5409..6128
                     /locus_tag="VM692_16895"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUQ53908.1"
                     /translation="MRQWVGSVLFTLILFVSVPIYGTLVFVATAWLPRRCTYAASAAW
                     VDFVLALLRVLCRLDYRVEGREHLPARSSIVLLKHSSAWETIAQLRIFPPQTWVLKRE
                     LMWAPVLGWVLWLLRPIAIDRRGGGVAVQQVLEQGRARLAEGLWVMIFPEGTRMPVGL
                     TRRYGMSGALLAAAEGLPVVPVTHNAGFFWPRRGWLKKPGTIRVVIGPPIETKNVEPR
                     LVNERAQQWIEQTLARLAPQD"
     gene            complement(6153..>7061)
                     /gene="gyrB"
                     /locus_tag="VM692_16900"
     CDS             complement(6153..>7061)
                     /gene="gyrB"
                     /locus_tag="VM692_16900"
                     /inference="COORDINATES: protein motif:HMM:NF036694.4"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HUQ53909.1"
                     /translation="VSHIRTLLLTFFSRQMPELIERGHIYIAQPPLYKLKRGKQEHYV
                     KDDGELNSLLLTSATADASLYADAGAPGIQGATLEKLALHYMEANSIIQRWGRRYDAT
                     VLRTLMSMGPIAPTELDDAERRRQWAQRLEVELRKAADDGAQYRVRAAQGGEGADPSL
                     VVTREHHGSQSTKTLHAGFFRSPEYQRLSAIGTELKDVLRAGAYVERGKERRDVQTFA
                     EAIEWLMTQAKQGQTIQRYKGLGEMNPEQLWDTTVNPETRRLMQVRIDDAVAADEIFT
                     TLMGDQVEPRREFIERNALAVANLDV"
BASE COUNT         1182 a         2226 c         2351 g         1302 t
ORIGIN      
        1 cggtggcgct cgccgccgac cgtgaaattc gtcagcgcgc gcgtgcgaaa gatcactcgg
       61 ccgcgctcgc cgagcgcctg catggcgtta cgtccgacat tgaggagggc ctgaatcagc
      121 tgattgcgat cgacggtgat aggcggcagg ctcgggtcgt aatcccgcac gagctcgatg
      181 cccgccgcct tctcggcgtc gatgagctgc gcgacgtgct cggtgatgcg gtgcacattg
      241 atctcttcct gcttcggcgc accgcccgca cgcaggatgc cgtcgacgag cgccgcgagc
      301 cgatcggctt cttctatgat cacgcgcgtg taggcgcgtt gctcctgatc cggcagctgc
      361 cgctcgagaa gctgcgccgc gccgcgcaaa ccgcccaacg ggttcttcac ctcgtgcgcg
      421 agctgccgaa tgatgcgacg cgataggcgc tgctgcgcca cgagctccgc ttcgcgccgc
      481 acgcggcgat cgcgttcggt atcgaggaac tcgaggacca acgagtgctc gttgtcgagc
      541 agcgcgacgc ggcagtcgag cacgatctcg cggtcgccga cgcgcacggc gagctcgtgc
      601 aagctgaacg agagccccgt cgcgagcgcg cgttgacaca aaacgagcaa ctcttgcgcg
      661 gggtgcacga tctccgtgag cgcgcggccg accgtgcgtt tcgagctcac gcctagcagg
      721 ttctcggcgg ccgagttcac ataggtgacg agcagcgcgt cgtcgagcac gacgagcgcc
      781 gtcgaaagtc cctgcaggat gttgtcggca ccgggcgcgc gtcgcgcgcg ccggcctggg
      841 cccgcgagct ccccgtggcc gcttagttgg cgccggcccg cgcccggccg ttcgccgagt
      901 tcggattcag cacgctggtt tgctggacga aaaacgttcg cggcgccgag cgcgtcactt
      961 ccgcgcccgc ggcatcgatg acgacgactt ggagcgtgtg gctgccgcgg acgatatccg
     1021 gcaacgcgac acgggtggtc gtcgtgttga cgttgcgccg ttgtccatcg aaagcgaggt
     1081 cgaagcggtg gcccgcttgc agagcgggtt gaaaacgtac ttgcacgttc aggaccccgc
     1141 cgacattcca aagcgtctct tgatcggcgg ggctcacaat gtcgatggct tggtaaaggc
     1201 tgccggcctg ctcgtccgag gccgtagtgc ggggcgcgct ccgcacggcg ccggccgagc
     1261 caaaaccctg tgcaccggac aactccactc gacgagcgcc ttccaccggc cgatcggaaa
     1321 agtgcaccgt gccgcttgcg tcggtccacg tccaagccgg cgccccgttc acggcggtcg
     1381 ctgagaacgc ggacaatagg atcagccact tacgcatgct ccaagcttag cgccgagcgg
     1441 cgttttctgc caacggtagg cggggttcct gagaaccccg ccacgttttc cgtttgacaa
     1501 aaccgcgata cgagcccgac tcagaggctg tagtacatct cgaactcgac gggatgcggc
     1561 gtcatcgaga agcgagcaat ctcctgctcc ttcaagccga tgtaggcatc gatcgtcgag
     1621 tcgttgagca cgccgccggc cgtcaagaag gcgcgatcct tgtcgagcgc ttcgagcgat
     1681 tgcgtcagcg agtacgacac cgtcggaatc agcttctcct cctcgggcgg caagtcgtag
     1741 aggtccttgt cgctcggcgg gcccggattg atcttgttcg cgacgccgtc gagaccggcc
     1801 atcagcatcg ccgtgaaccc gaggtacggg ttcgacgtgc tgtccgggaa tcgcacttcg
     1861 atgcggcgcg ctttcgggtt ctgcacgtac ggcacgcgaa tcgacgcgga acgattacgc
     1921 gcggagtacg ccagcagcgt cggagcctcg aagcccttca cgagacgctt gtagctgttc
     1981 gtcgaagcgt tcgtcagcgc gttgatcgcg tgcgcatgct tcatgatgcc gccgatgtag
     2041 tagagcgccg tctgcgacag gccgccgtac agatcgcccg tgaacaggtt cttgccgccc
     2101 ttcgcgagcg attgatggac gtgcatgccg ctgccgttgt cgccgacgag cggcttcggc
     2161 atgaacgtcg ccgtcttacc gaagctgtgc gcgacgttat ggatgacgta cttcaggatc
     2221 atgacgtcgt cggccttttt caccagcgag ccgaacttga cgccgatctc gcactggccg
     2281 gccgtggcga cctcatggtg gtgcacttcg acgacgaggc ccatttcctc gagcgccagg
     2341 cacatcgcgg accgaatgtc ctgcagggag tcgaccggcg ggaccgggaa gtagccgccc
     2401 ttgatgccgg gacggtgccc gagattgcct tcctcgatct ccttgccgct ctgccacgcg
     2461 ccctcgtacg actcgatcgt gtagccgccg ctatgcatct gcgagtgcca acgcacgtcg
     2521 tcgaaaatga agaactcgtt ctcgggaccg aagaacgcct cgtcggcaat gccggtcgag
     2581 cgcatgtagg cttcggcgcg tttcgccgcg gagcgcggat cgcgctcata gccttgcatc
     2641 gtcgacggct cgatgatgtc gcagcggatg ttcagcgtcg tctcttccat gaatgggtcg
     2701 aggatcgccg tcgacggatc gaggccgagg atcatgtcgg actcgttgat gcccttccaa
     2761 ccggcgatcg atgagccgtc gaacatcttg ccgtcctcga atacgccgag gtcgatggtg
     2821 tgcgacggga cggtgacgtg ttgttctttg ccgcgcgtat ccgtgaaacg cagatcgacg
     2881 aacttgacgc ctttttcctt gatgagcttg agaacatcgg cgggcgacat gaattcctcc
     2941 gttagtgctg ctttaaagtt gttgtcgctg ccggtgctat ttcggtggtc tgtgcacggc
     3001 agttggccct acaaaaagag ccccaagatg tgcattcttt gtgccacgct cgggacgcgg
     3061 taacttactg ttttgtctga tgctatgcaa caaccggttg agttcctgct ccggaatggt
     3121 gcggcgcgtc gtttatttcg cccgattata gtgcacggta gcgcgccgca cggcggccca
     3181 attcgactgc tggcgcagac aagggcgcac gagcagcgcc cgctacggcg ctgacgcgtc
     3241 gttatacttg cgcgctttga tttggctagc cgctcggaga agtcatggac gcaatggaaa
     3301 aaatagtgtc gctgtgcaag cgacgaggct tcatcttccc ttcgagcgag atctacggcg
     3361 gtttgaacgg cgcgtgggac tacggcccgc tcggcgtcga gctcaagcgc aatctgaagc
     3421 aggcgtggtg ggacgacatg gtcgcgcatc acgacgagac ggcgacgccc gccggtgcgc
     3481 cgtcagggtt cggcatggtc ggcctcgaca gctcgttact gatgaatccg aaagtctggg
     3541 aagcgagcgg ccacgtcggc gggttcagcg atccgatggt cgacgaccgc gagactcggg
     3601 cgcgctaccg cgccgaccag ctcgccgtat acgcactcga attcatcggc ggcgacggca
     3661 agctcgagcg ttacgacgag gtgctgtttg cggcgttggg ggaggtcgga aagaccgggc
     3721 ctgcggagct gttcgagccg catcgtaagc gcatcgccgg cttacagaag agcaaaggcg
     3781 gaaaggaggc ccggcccatt cccgtgcccg gcgcgttgca ggacgagacg ttgcgcgcga
     3841 aggtgctctc gcccggtgca tcggcgccgg gcacgctgac ggcgccgcgc gccttcaacc
     3901 tcatgttcaa gacgcacgtc ggcgcgctcg aggacaactc gagcgtcacg tacttgcgcc
     3961 ccgagacggc gcagggaatc ttcgtcaact tcaagaacgt ctgcgacacg gcgcgcgtgc
     4021 gcgtgccgtt cggcataggc caagtcggta aagcgtttcg caacgagatc aacccgcgca
     4081 atttcacgtt tcgcagccgc gagttcgagc agatggagat cgagtttttc tgtcggccga
     4141 acgaagccgc gcagtggtat cagtactggc gcgagcgacg ttatcgctgg tatctcgacc
     4201 tcggcatcga gcaagcgaac ttgcatctgc gcgagcacga ggcgaacgag ctcgcgcact
     4261 acgccgcggg ctgtgccgac gtcgagtacg actttccgtt cggccgcagc gagctcgaag
     4321 gaatcgccaa ccgcacggat ttcgatctgc gtcagcacat ggagaagagc ggcaaagacc
     4381 ttcgctattt cgacgatcag gagacgaacg aggaggcgcg gcgttatctg ccgcacgtca
     4441 tcgagccgtc ggccggcgcc gatcgcggca cgctggcgtt cctgtgcgac gcctacagcg
     4501 aggaccaggt cggcggcgag ccgcgcacgg tgctcaagct tcatccgcgg ctcgcaccga
     4561 tcaaagctgc ggtgtttccg ctcgtgaaga aggacggcat gccggagaag gcgctcgaga
     4621 tttaccgcac gttgaagaag cgcttcagcg cctattacga cgagaagggc gcgattgggc
     4681 ggcgctatcg ccgacaagac gaggcgggca cgcccttctg catcaccgtc gacggcgaca
     4741 cgctgacggc cgggaccgtg accgtgcgcg agcgcgacag ctgcgagcaa acgcgcgtgg
     4801 cagtcggcga gcttgccgac gtgctcggcg agcggatcgg ggcctaacgc cgtggcgcgc
     4861 agcgccgggc tcgtgatgat cgaccgcgac ggcgtcatca acgaagactc gggcgagttc
     4921 atcaagtccg tgtcggagtg gcgcccgatc gaaggaagtc tcgaggcgat cgcggcattg
     4981 caccacgcgg gctggcgcgt cgtcgtcgtc acgaatcagt cgggcatcgg tcgcggactc
     5041 tatgacgagg ccgcgcttgc ggcgatccat cggcacatgc gcgagcgcgt gcgcaacgcc
     5101 gggggcgagc tcgccggcgt ctactattgc ccacacctgc ccgaggacgg ctgcgactgc
     5161 cgcaaaccga agcccggtat gttccgcgca ctcgagcgcg agctcggtgt gtccgttcgc
     5221 ggttcgccgt acatcggcga ccgcatcagc gacatcgaag cggcggaggg cgtcggcgcg
     5281 cgaccgattc tcgtgcgcac gggcacgggc gccgcgacgg agggcttact ggcggggcgg
     5341 aacgtgccgg tgttcgacga ccttgcggcc gccgctcgcg acttgctcga caagacgagg
     5401 tgacgcactt gcggcagtgg gtcggttccg tgttgttcac gctgatactg ttcgtgtccg
     5461 tgccgatcta cggcacgctg gtgttcgttg cgacggcgtg gctgccgcgt cgttgcactt
     5521 acgccgcctc ggccgcgtgg gtcgacttcg tgctcgcgct gctacgcgtg ttgtgccggc
     5581 tcgattaccg cgtcgaggga cgcgagcacc tgcccgcgcg cagcagcatc gtgctgctca
     5641 agcactcgtc ggcgtgggag acgatcgcgc agctccggat ttttccgccg cagacgtggg
     5701 tgctgaagcg cgagctgatg tgggcgcccg tcctcggctg ggtcctgtgg ctactgcgac
     5761 cgatcgcgat cgaccgccgt ggtggcggcg tggccgtgca gcaagtcctc gagcaagggc
     5821 gcgcgcgtct cgccgaagga ttgtgggtca tgatttttcc cgaggggacg cggatgcccg
     5881 tcggcctgac gcgtcgctac ggaatgagcg gcgctctcct cgccgccgcg gaagggttac
     5941 cggtcgtgcc cgtgacgcac aacgcgggat tcttttggcc gcgccgcggt tggttgaaga
     6001 agcccggcac gattcgcgtc gtaatcgggc cgccgatcga aacgaagaac gtcgagccgc
     6061 ggctcgtcaa cgagcgggcg cagcaatgga tcgaacagac gctggcgcgc ctagcgccac
     6121 aagactagac gacgacccac ggccggcgct cttcagacgt cgagattggc gaccgctagc
     6181 gcgttgcgct cgatgaactc gcggcgcggc tcgacctgat cgcccatcag cgtcgtgaag
     6241 atctcatcag ccgcgacggc gtcgtcgatg cgcacttgca tgagccgccg tgtctccgga
     6301 ttgacggtcg tatcccaaag ttgctcggga ttcatttcgc cgagaccttt gtagcgctga
     6361 atcgtttggc cctgtttcgc ttgcgtcatg agccattcga tcgcttcggc gaacgtttgc
     6421 acgtcgcgcc gttccttgcc gcgctcgacg tacgcgcccg cgcgcaacac gtctttgagc
     6481 tccgtcccga tcgccgacag gcgttggtac tccggcgaac gaaagaaacc tgcgtgcagc
     6541 gtcttcgtgc tctgcgagcc gtggtgctcg cgtgtcacga cgaggctcgg gtcggcgccc
     6601 tcgccgcctt gcgcggcccg cactcggtat tgcgcgccat cgtccgccgc cttgcgcagc
     6661 tcgacttcga gccgttgcgc ccactggcga cggcgctcgg cgtcgtcgag ctccgtaggc
     6721 gcgatcggcc ccatcgacat cagcgtgcgc aggacggtcg catcgtagcg gcgaccccag
     6781 cgctgaatga tggagttcgc ttccatgtaa tgcagcgcga gcttttcgag cgttgcgccc
     6841 tgaattcccg gcgcgccggc gtccgcgtat agcgacgcgt cggccgtggc gctcgtcaac
     6901 agcagcgagt tgagctcgcc gtcgtccttg acgtagtgct cttgcttgcc gcgtttgagc
     6961 ttgtacaacg gcggttgcgc gatatagata tggccccgct cgatgagctc gggcatctgg
     7021 cgggagaaga acgtcagcag cagggtgcgg atgtgggaga c
//
LOCUS       DATLEE010000768         3220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10305618, whole genome shotgun sequence.
ACCESSION   DATLEE010000768 DATLEE010000000
VERSION     DATLEE010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10305618"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="maeA"
                     /locus_tag="VM692_16905"
     CDS             <1..963
                     /gene="maeA"
                     /locus_tag="VM692_16905"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HUQ53910.1"
                     /translation="QWEDFRKDNALTILDRYSSVLPSFNDDIQGTGAVVMAGLNSALK
                     IKNERLSQQRIVIFGAGAAGLGVSRQIRAQLVDEGVIGDAATGVIAVLDRAGLLVDDG
                     RPMDEYKRALAWSAERATALNLAAPTERSLLDVVRKYRPTVLIGLSGVAGAFNEAVVR
                     EMAKHVERPAIFPSSNPSASSEAVPADLYRWTDCRCLVATGSPFPNVDCNGKVYRVGQ
                     GNNVFIFPGLGLAAIAANARKVTDAMTKVASEALAAQVTNEEREAALLFPSVTRLRAV
                     SFEIAVAVARQAIKDGVAGCKLADVERLVREARWSHDYADYVPA"
     gene            complement(973..1836)
                     /locus_tag="VM692_16910"
     CDS             complement(973..1836)
                     /locus_tag="VM692_16910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUQ53911.1"
                     /translation="MRAIVNGRWLLGFVACLAATLGSRPCAAEVAPHYAELPVGRVFY
                     LDSGGEGDVVVFLHARSGNSLLFERQIEPFTKAGYRFIAFDRVGQGRSTRASDAPSAD
                     ARPELEQLMDHLAIARAHLVGVAAGGGVVLEYVIAHPQRVASITIANSVGNVQDPDYL
                     ALGRRLRPPEFDRLPLELRELGPSYRAANVEGVRRWLELSTSGQQSPAPQTVGAVPVT
                     FAALAELDVPTLLLTGDADLYTPPAVLRLFNEHMPRAELVIVPDSGHAANWENPEAFN
                     RAVLRFLRKRR"
     gene            1917..2303
                     /locus_tag="VM692_16915"
     CDS             1917..2303
                     /locus_tag="VM692_16915"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ53912.1"
                     /translation="MTKEEFAVYLRALQAHDYDAIKSYYTHDYCAHFDGATFDRDGVI
                     EVERALASVADSGWDVLDIVADANAIAVHAFLEMRFKKDAPPGFPLGDFKAGQRIKTR
                     FCGFYKLRDGRISEFRVFPFLGEKLT"
     gene            complement(2368..>3220)
                     /locus_tag="VM692_16920"
     CDS             complement(2368..>3220)
                     /locus_tag="VM692_16920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HUQ53913.1"
                     /translation="VFVAIAVGANTAIAVLCAAMAWSVSSPEWAMLAPLAMWAPALAR
                     LVTRSTVDRGFTSTLTLNRWGDGGALVILRPLAIPLLVYGAAYAIAWSVGAAQWSPGE
                     GRWTSAPQILANLVINLSILVVYGTFTATGEELGWRGYLQPRLDAAGVRYSVLVVWVV
                     QLAYHAPLMIGAGYLGSDSLVQSLLLFAIGDLPVTFLIAWLAYRARSLWPAVFLHSFH
                     NTVSQWLFAKFFIGDENPLVLGEGGILPMAGYVIVGVVVYVAMRRRGLSWAQLASAAF
                     RADAART"
BASE COUNT          584 a         1118 c         1041 g          477 t
ORIGIN      
        1 cagtgggaag atttccgcaa ggacaacgcg ctgacgatcc tcgatcggta ttcgagcgtc
       61 cttccgtcgt tcaacgacga tatccaaggc acgggcgccg tcgtcatggc cggcctcaac
      121 agcgcgctga aaatcaaaaa cgagcgtctg tcgcagcaac gcatcgtgat cttcggcgcc
      181 ggcgcggcgg gcctcggcgt ctcgcgacag attcgcgcgc agctcgtcga cgaaggcgtc
      241 atcggcgacg ccgcgaccgg cgtcatcgcg gttctcgatc gcgcggggct gctcgtcgat
      301 gacgggcggc cgatggacga gtacaagcgc gctttggcgt ggtcagcgga gcgcgccacg
      361 gcgctcaatc tcgcagctcc gacggaacgc agcttgctcg acgtcgtccg taagtaccgg
      421 cccacagtct tgatcggcct ctccggcgtc gccggcgcgt tcaacgaagc cgtcgtccgc
      481 gagatggcga agcacgtcga acgccccgcg atcttcccgt cgtcgaaccc gagcgcgagc
      541 tcggaggccg tgccggccga tctttatcgc tggacggatt gccgctgcct cgtcgcgacg
      601 ggcagcccgt tcccgaacgt cgattgcaac ggcaaggttt accgcgtcgg tcagggcaac
      661 aacgtcttca tcttcccggg cctcggcttg gccgcaatcg cggccaacgc gcgcaaggtc
      721 acggacgcga tgactaaggt cgcctcggaa gcgctggcgg cgcaggtcac gaacgaagag
      781 cgcgaggctg cgctgctatt cccttccgtg acgcgcttgc gcgcggtctc gttcgagatc
      841 gccgtggccg tcgcgcgcca ggccatcaag gacggcgtcg ccggctgcaa gctggccgac
      901 gtggagcgcc tcgttcgcga ggctcgttgg tcgcacgact acgccgacta cgtgcccgcc
      961 tagccggccc gcctagcgcc gcttgcgcaa gaagcgcagc acggcacgat tgaacgcctc
     1021 ggggttttcc caattcgccg catgaccgct gtcgggcacg atgacgagct cggcgcgcgg
     1081 catgtgctcg ttgaataacc gcagcacggc cggcggcgtg tagagatccg cgtcgcccgt
     1141 cagcagcagc gtcggcacgt cgagctccgc gagcgccgcg aacgtgacgg gcacggcgcc
     1201 gacggtttgc ggcgccggcg attgttgacc cgacgtcgac agctcgagcc aacgtcgcac
     1261 gccttcgacg ttcgcagcgc gatacgacgg accgagctcg cgcagctcga gcggcaaccg
     1321 gtcgaactcg ggcggccgca ggcgcctgcc caacgcgaga tagtcgggat cctgaacgtt
     1381 gccgacgctg ttcgcgatcg tgatgctcgc gacgcgctgc ggatgcgcga tgacgtactc
     1441 gagcacgact ccgccgccgg ccgccacgcc gacgaggtga gcccgcgcta tcgccaaatg
     1501 atccatgagc tgctcgagct cgggccgcgc gtcagccgac ggcgcatcgc tcgcgcgcgt
     1561 cgagcggcct tggccgacgc gatcgaaggc gatgaaccgg taaccggctt tcgtgaacgg
     1621 ctcgatctgt cgctcgaaca gcaagctgtt gccgctgcga gcgtgcagga acacgacaac
     1681 gtcgccctca ccgccggaat ccagatagaa cacgcggccg accggcagct cggcatagtg
     1741 cggggccacc tccgcggcgc acggccgcga gcccagcgtc gcggcaagac acgcgacgaa
     1801 cccgagcaac caacggccgt tcactattgc gcgcatgctc cgcgagtcta gtcgctgtgc
     1861 agccggccgc aaccgcggcg ttcgctatac tcgcgcgacc ggccacgagg cgagccatga
     1921 cgaaagaaga attcgcggtc tatctgcgcg cactacaggc gcacgactac gacgccatca
     1981 agagttacta cacgcacgac tactgcgcgc acttcgacgg tgcgacgttc gaccgcgacg
     2041 gcgtcatcga ggtcgagcgc gcgctcgcat ccgtcgccga ctccggctgg gacgtgctcg
     2101 acatcgtcgc agacgcgaac gcgatcgccg tgcacgcgtt cctcgagatg cgcttcaaga
     2161 aggatgcgcc gccgggcttc ccgctaggcg acttcaaagc gggccaacgc atcaagacgc
     2221 ggttctgcgg cttctacaag ttgcgcgacg gcaggatcag cgagttccgc gtctttccgt
     2281 tcctcggcga gaagctgacc tagcgatcgc taaaactcga agtagcgacc gccggattcc
     2341 ctccgtgcag ggcgactccc aatcccttca ggtgcgtgca gcgtccgccc ggaacgccgc
     2401 gcttgcgagc tgcgcccacg acagcccgcg ccgccgcatc gcgacgtaca cgacgacgcc
     2461 gacgatcacg tagccggcca tcggcaagat tccgccctcg cccaggacga gtgggttctc
     2521 gtcgccgatg aaaaacttcg cgaacagcca ttgcgagacc gtgttgtgga agctgtgcag
     2581 gaacacggcg ggccagaggc tgcgagcgcg atacgcgagc caggctatca gaaacgtcac
     2641 cgggagatcg ccgatcgcga agagcagcag gctctgcacc aagctgtcgc tgcccaagta
     2701 gcccgcgccg atcatcagcg gcgcgtgata ggcgagctga accacccaga cgacgagcac
     2761 gctgtagcgc acgccggcag cgtccaagcg cggctgcagg tagccgcgcc aaccgagctc
     2821 ctcacccgtc gcggtgaacg tgccgtagac gactaggatc gataggttga tgactaggtt
     2881 ggccagaatc tgcggcgcgc tggtccatcg gccctcgccg gggctccatt gtgcggcgcc
     2941 gacgctccat gcgatcgcgt acgccgcccc gtacaccagc agcggaattg cgagcggccg
     3001 caggatcacg agcgcgccgc cgtcgcccca acgattcagc gttagcgtcg acgtgaagcc
     3061 gcgatcgacc gtgctccgtg taacgaggcg cgcgagcgcc ggcgcccaca tcgcgagcgg
     3121 cgccaacatc gcccactcag gcgagctcac cgaccacgcc atcgccgcgc aaagaacagc
     3181 gatagccgtg ttggcgccaa cggcaatcgc gacgaacacc
//
LOCUS       DATLEE010000769         3015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8078832, whole genome shotgun sequence.
ACCESSION   DATLEE010000769 DATLEE010000000
VERSION     DATLEE010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8078832"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="VM692_16925"
     CDS             <1..1412
                     /locus_tag="VM692_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HUQ53914.1"
                     /translation="GVTDYELMCRAAAAAVRSIEQRWPSARSLAIVCGAGNNAGDGLV
                     LARLAQAAGLTVSVLLVAGERFKGAAAQAAADCRSAAIPFTAFEARGLANADVIVDAL
                     LGTGLARPVSDLFRAAVEAINAAGAPVLALDIPSGLDADLGWPSPVAVRATATLTFLG
                     LKQGLFLGAAIDHCGELEFAGLELPVSLGAELPAPLRRLLFDDLRRALPRRPRSAHKG
                     SCGRLLLVGGGPGMPGAIRLAAEAALRCGAGLVYVATHRDSAASVLSGRPEIICSSVG
                     SGEDLDHLLRMVDGVVLGPGLGQSSWAKGLWRRVLETDLPLVVDADALNLLALENVER
                     GNWILTPHPGEAARLLPGGTVEGVQSDRVAAARALAARYRAIAVLKGPHTLVATHGVD
                     TLRVCDRGNPGMATGGTGDVLAGTLGSLLVQTGNLEQAAHAGVLLHALAGDEAAAEGE
                     RGTVAGDLLPHLRAWANPR"
     gene            1394..1900
                     /gene="tsaE"
                     /locus_tag="VM692_16930"
     CDS             1394..1900
                     /gene="tsaE"
                     /locus_tag="VM692_16930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HUQ53915.1"
                     /translation="MGEPALSVELGSEQAFAELAASCARAWRAARAWPLVLGLTGDLG
                     SGKTTWIRAMLRALGYSGRVPSPTYTLLEQYACGELTVVHLDLYRLRGGDELEHLGMR
                     DWLAEPVCWIAVEWPERAPQLAARCDLTLAFGVTGPTGRRIEASAATGLGIEALRSLR
                     HGAFNNKP"
     gene            2026..>3015
                     /locus_tag="VM692_16935"
     CDS             2026..>3015
                     /locus_tag="VM692_16935"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HUQ53916.1"
                     /translation="MLLLVVGSVASAATVQSVRAWSGPEGTRVVFELSGPVDHRVFAL
                     ANPDRVVIDLPSTSAASGLTLADPKGVVTALRTGARAGGELRLVLELNTPAKPKTFLL
                     APHEEYGYRLVIDLPPAEFAAVVRRVPQPSTDRGRDVVVVIDAGHGGEDPGASGRGGA
                     REKDVVLAIARKLAAEVNAQPGMRAVLVRDGDYFVSHRKRMEIAHGAHADFFMSIHAD
                     SYKSADAKGATVYVLSDKGATDEAALLLAQRENASDLIGGVSLADKDQLLARVLLDLS
                     QSAALNASTAAGQRLIRRMSTVTAMRRREVQQAPFLVLKSPDIPSVLVETAYIS"
BASE COUNT          423 a         1013 c         1089 g          490 t
ORIGIN      
        1 gcggcgtcac ggactacgag ctgatgtgcc gtgccgcggc cgccgcggtg cgcagcatcg
       61 agcagcgctg gcctagcgct cgctcgctcg cgatcgtctg cggcgccggc aacaacgccg
      121 gcgacggcct cgtgctcgcg cggctcgcgc aggcggccgg gctcaccgtg tcggtgttgc
      181 tcgtcgccgg cgagcggttc aagggcgcgg cagcgcaagc cgccgccgac tgccgctccg
      241 ccgcgatccc gttcaccgct ttcgaagcgc gcgggctcgc gaacgccgac gtcatcgtcg
      301 acgcactgct gggcacgggc ctcgcgcgcc ccgtcagcga ccttttccgc gccgctgtcg
      361 aggccatcaa tgccgcgggc gcccccgtgc tcgcgctcga cattccgagc gggctcgatg
      421 cggacttggg ctggccgagt cccgtagccg tgcgcgcgac ggccacgctg acgttcctcg
      481 ggctgaagca ggggctgttt ctcggcgcgg ccatcgacca ttgcggcgag ctcgagtttg
      541 cgggactcga gctgccggtt tcgctcgggg ccgagctccc ggcgccgcta cgccggctgc
      601 ttttcgacga cttgcggcgc gcgttgccgc gccggccgcg cagcgcgcac aaagggtcgt
      661 gcgggcggtt gctgctcgtc ggcggcgggc ccggcatgcc gggggcgatt cgcttggcgg
      721 ccgaggcggc gttgcgctgc ggcgccggtc tcgtctatgt cgcgacgcat cgcgatagcg
      781 ccgcgagcgt gctgtcgggc cgccctgaaa tcatttgcag ctcagtcggt tctggggaag
      841 acctcgacca tcttctgcgc atggtcgatg gcgtcgtcct cgggccgggc cttggtcaat
      901 cgtcatgggc gaaaggtctc tggcggcgcg tgctagagac cgacttgccg ctcgtcgtcg
      961 atgcagacgc gctgaacctg ctggcgctcg aaaacgtcga gcgagggaac tggatcctga
     1021 cgccgcatcc cggcgaggcg gcgcggctgt tgcccggcgg tacggtcgaa ggcgtgcaaa
     1081 gcgaccgtgt cgccgccgcc cgcgcgctcg ctgcgcggta ccgcgcgatt gcggttttga
     1141 agggccctca cacgctcgtc gcgacgcacg gcgtggacac gctgcgcgtc tgcgatcgcg
     1201 gcaaccccgg catggcgacc ggcggcacgg gcgacgtgtt ggccggcacg ttgggatcgc
     1261 tgctcgtgca gacgggcaat ctcgagcaag ccgcgcacgc gggggtgctg ttgcatgcgc
     1321 tcgccggaga cgaagcggcg gcggaagggg agcgcggcac cgttgccggc gatctgttgc
     1381 ctcacctgcg agcatgggcg aacccgcgtt gagcgtcgag ctcggctccg agcaggcctt
     1441 cgccgagctc gctgcgagtt gtgcgcgagc atggcgggcg gcccgcgcgt ggccgctcgt
     1501 gctggggctg accggcgacc tcggcagcgg caagacgacg tggatccgcg ccatgctgcg
     1561 cgcgctcggc tacagcggcc gcgtgccgtc gccgacctac acgctgctcg agcagtacgc
     1621 gtgcggcgag ctgaccgtcg tgcacctcga tctctatcgg ctgcgcggcg gcgacgagct
     1681 cgagcatctc gggatgcgcg actggctggc tgagccggtc tgctggatcg ccgtcgagtg
     1741 gccggaacgc gcgccgcagc tcgcggcgcg ctgcgatctg acgctggcct tcggcgtcac
     1801 gggaccgacg ggccggcgaa tcgaggcaag cgccgctacc ggcctgggaa tcgaggcgtt
     1861 aaggtccctt cgtcacggag cgtttaataa caagccgtaa ttgcctgctc tcaaacagga
     1921 aaccgttgaa aatcggacta aaactcggta aacataagta gtcgtggggc gccagggagt
     1981 aggagcgaag tggtacgggg ggtgaggctg cggtggtgcg ccggcgtgct gctcctcgtt
     2041 gtaggcagcg tcgcgagcgc ggcgaccgtg caaagcgttc gcgcctggtc cggccctgaa
     2101 ggcacgcgcg tcgtcttcga gctcagcggc cccgtcgacc atcgcgtctt cgcgttggcc
     2161 aaccccgacc gggtcgtcat cgacttgccg agcacgtcag ccgcttcggg cctgacgctc
     2221 gccgatccca aaggtgtcgt cacggcgctg cgcaccggcg cgcgggccgg cggcgagctg
     2281 cggctcgtgc tcgagctcaa tacgccggcg aagcccaaaa cctttctact cgcgccgcac
     2341 gaagaatacg ggtatcgact cgtgatcgac ttgccgccgg ccgaatttgc tgccgtcgtc
     2401 cgccgagtac cgcaaccttc gaccgatcgc ggtcgcgacg tcgtcgtcgt catcgatgcc
     2461 ggccacggcg gcgaggaccc gggtgccagc ggccgcggcg gcgcgcggga gaaggacgtc
     2521 gtgctcgcga tcgcgcgcaa gcttgctgcc gaggtcaacg ctcaaccggg catgcgcgca
     2581 gtgctcgtgc gcgacggcga ctacttcgtc tcgcatcgca agcgaatgga gattgcgcac
     2641 ggcgcgcacg ccgacttctt catgtcgatt cacgccgact cctataagag cgccgatgcg
     2701 aagggcgcga cggtctacgt gttgtcggac aaaggcgcga ccgacgaagc ggcgttgttg
     2761 ctcgcgcagc gcgagaacgc gtccgacttg atcggcggtg tttcgctagc ggacaaagac
     2821 caattgctcg cgcgcgtgct gctcgatctg tcacagagcg cggcgctgaa cgcgagcacg
     2881 gcggcggggc agcgcctgat ccggcgcatg tcgaccgtga ccgcgatgcg gcggcgcgag
     2941 gtgcagcagg cgccatttct cgtgctgaaa tcgccggaca ttccctccgt gctcgtggag
     3001 acggcgtaca tctcg
//
LOCUS       DATLEE010000770         2156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8086020, whole genome shotgun sequence.
ACCESSION   DATLEE010000770 DATLEE010000000
VERSION     DATLEE010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8086020"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VM692_16940"
     CDS             <1..1013
                     /locus_tag="VM692_16940"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HUQ53917.1"
                     /translation="IIAEIGAHKIQNSVPTRDYEREREVLKGAETQARELGLEPELAG
                     EIMALLIRASLTHQEQRRVAAGTSGAGKRVLIIGGAGKMGAWFAHFLSSQGFAIEIAD
                     PSPVAVGFARVADWRECPLEHDIIIVATPMKVAGDVLAELAARRPRGVVVDIGSLKSP
                     LRSGLERLQAAGCKVTSIHPMFGPDTRLLSGRHVVFCDVGVPEATRAARALFASTMAE
                     QTEMSVDDHDRLMAYVLGLSHALNLAFFTALAASGELVPRLQKMSSTTFDAQLRVASL
                     VARDNPHLYFDIQTLNDYGAASLQALRAATERIERLVQGGDEAGFVELMAAGRRYLES
                     RT"
     gene            1035..2135
                     /locus_tag="VM692_16945"
     CDS             1035..2135
                     /locus_tag="VM692_16945"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HUQ53918.1"
                     /translation="MDREDHDRELTLLRRRLAVLTDEARKNDDAWRRAQAREMELLEA
                     DTLDALLERLTAGLRSSYRLEAATLVVADLDHEIRRLLFASAPAAEHSSVLFVDSVHA
                     VAPAVAAGRRPWLGKFNRADHALLFPAKTDLESVALLPLTRQERLIGCLNFGSRQRER
                     FQAALGTEFLHHLAVIAAFALESAVNRARLVRSGFTDVLTGWHNRRYLQTRLVEELAR
                     CRRERTPLTCLMIDVDHFKSVNDRFGHLAGDEVLRQLAQCISGDVRGSDVSARYGGEE
                     FVVLLPGTGAAAGFLLAERIRAAVAADPFELPGGVPPLPVTVSIGVSEHSPTPDEVDL
                     LVVGERLLALADVALYEAKAGGRNAVARAANS"
BASE COUNT          329 a          700 c          770 g          357 t
ORIGIN      
        1 aaatcatcgc ggagatcggg gcccacaaga tccagaactc cgtgccgacg cgcgattatg
       61 agcgggagcg cgaagtgttg aaaggcgcgg agacgcaagc gcgtgagctg ggcctcgagc
      121 ccgagctcgc gggcgagatc atggcgctgc tgattcgcgc gtcgttgact caccaggagc
      181 agcggcgcgt cgcggccggc acgagcgggg ccggcaaacg cgtgctgatc atcggcggcg
      241 cgggcaagat gggcgcctgg ttcgcgcact tcttgagctc ccaaggcttc gccatcgaga
      301 ttgccgatcc gagccctgtg gccgtcggct ttgcgcgcgt ggccgactgg cgcgaatgtc
      361 cgctcgagca cgacatcatc atcgtcgcga cgccgatgaa ggtcgccggc gacgtgctgg
      421 ccgagctcgc cgcgcggcgg ccgcgcggtg tcgtcgtcga catcggctcg ttaaagagcc
      481 cgttgcgcag cgggctcgag cggctgcaag cggctggctg caaggtcacg tcgattcacc
      541 cgatgttcgg ccccgatacg cgcttgctgt cgggccggca cgtcgtgttc tgcgacgtcg
      601 gcgtgccgga ggcgacgcgc gcggcgcgcg cgctgttcgc gtcgacgatg gccgagcaaa
      661 ctgagatgag cgtcgatgat cacgatcggt tgatggcgta cgttctcggc ttgtcgcacg
      721 cattgaacct ggccttcttc acggcgctcg cggcgagcgg cgagctcgtg ccgcggctgc
      781 aaaagatgtc gagcacgacg ttcgacgcgc aactgcgagt ggcttcgctc gtggcgcgcg
      841 acaacccgca cctgtatttc gacattcaga ccttgaacga ttacggcgcg gcctcgttgc
      901 aggcgctgcg ggctgcgacg gagcgcatcg agcggctcgt ccagggcggc gacgaggcgg
      961 ggtttgtcga gttgatggcg gcggggcgcc gctacctcga gagccgcacc tgagctcgag
     1021 ctaggaggtc acgagtggac agggaagacc acgaccgcga gcttacgttg ctgcgccgcc
     1081 ggctggcagt gctgacggac gaagcacgca agaacgacga cgcgtggcgg cgtgctcaag
     1141 cgcgcgagat ggagctgctc gaggccgata cgctcgatgc gctgctcgaa cgtctgacgg
     1201 ccggcctgcg atcgagctat cggctcgagg cagcgacgct cgtcgtagcc gacctcgacc
     1261 acgagatccg tcgcctgttg ttcgccagtg cgccggcggc cgagcacagc agtgtgctgt
     1321 tcgtggactc ggtgcacgcc gtcgcgccgg cggtcgcggc aggccggcgg ccgtggctcg
     1381 gcaagttcaa tcgcgcggat cacgcgctgc tgtttcctgc gaaaacggac ttagagagcg
     1441 tggcgctgct gccgctgacg cgccaggaac gattgatcgg ctgcctgaac ttcggcagcc
     1501 gccagcgcga gcgcttccaa gcggcgctcg ggacggagtt cctgcaccac ctcgccgtga
     1561 tcgccgcctt cgcgctcgag agcgcggtca accgcgcccg gctcgtgcgc agcggcttta
     1621 ccgacgtgct gacgggttgg cacaaccggc gctatctaca gacgcggttg gtcgaggagc
     1681 tcgcccgctg ccgtcgtgaa cgcaccccgc tgacgtgcct catgatcgac gtcgatcatt
     1741 tcaagtccgt caacgaccgg ttcggccatc tggccggcga cgaggtgctg cgtcagctcg
     1801 cgcagtgcat cagcggggac gttcgcggca gcgacgtgtc ggctcgttat ggcggcgaag
     1861 agttcgtcgt cctgctgccg gggacgggcg ctgcggccgg attcttgctg gccgaacgta
     1921 tccgggccgc cgtcgcagcc gacccgttcg agctgcccgg cggggtcccg ccgctccccg
     1981 tcaccgtttc gatcggggtg tccgagcaca gcccgacgcc cgacgaggta gaccttctgg
     2041 tcgtcggcga gcgactgctc gcgctcgccg acgtcgctct gtacgaggcg aaggccggcg
     2101 gacgcaacgc cgtcgcgcgg gcggccaata gctaattgag tcacaccttg gccagg
//
LOCUS       DATLEE010000771         4369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6553784, whole genome shotgun sequence.
ACCESSION   DATLEE010000771 DATLEE010000000
VERSION     DATLEE010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6553784"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VM692_16950"
     CDS             complement(<1..335)
                     /locus_tag="VM692_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010647926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ53919.1"
                     /translation="MDRRCGTLVGLALSCTLLAACRQPDTSLVALPTTSWPTNGGNWY
                     NQRHSPLTQIDRGNVATLKGVWRTQLGGSGVGPQYSGEAQPLVIDGVVYVPTGASDLF
                     ALDVTTGAIL"
     gene            complement(347..2428)
                     /locus_tag="VM692_16955"
     CDS             complement(347..2428)
                     /locus_tag="VM692_16955"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ53920.1"
                     /translation="MTQTSVRIGRSTRVRRALVAVVAIVASSLTAPLASQGIRPAPAF
                     KRDALVAAPTAGWPTNGGNWYNQRYSPLKQIDRDNVANLKGVWRTRLGGSGVGTKYSG
                     EAQPIVYDGVIYIVTGADDVFAIGVESGAILWSYAANLDAANDVVCCGWTSRGVGLGQ
                     GMVFVGQLDGKLVALDQKTGTVAWQVQAERWQDGLTITSAPLYYDGLVITGFAGAEYG
                     IRGRVKAFDAKNGKLVWTFYTIPGPGELGHETWPQDNDLWMHGGASVWQTPAVDPELG
                     LIFFSTGNPGPDYNGGVRAGDNLFSASIVALDAKTGQHRWHFQQVHHDIWDYDGPSPV
                     VLFDLELNGAMRLGLAQPSKTGWVYILDRTNGTPLVGIDERPVPQEPRQATAATQPYP
                     RGDSFVPQQIAVAPEGFALVNGGKIFTPFWTDREVPVKPSFGGGTNWPPSSYDPASGY
                     LYVCAQDRVGVFKADALSTERPPAGERYVAGIFGASGLPNFGVFAALDMHTNTLVWQQ
                     QWADACYSGSTTTAGGVTFVGRNDGRLTALDSKNGVRLWEFQTGAGMNAPVSVFEHAG
                     KQYVVAYSAGNLFAGSPKGDSVWLFGLDGTLPPATSPGAAMLFTREAEGTADPAAGKI
                     VYDTACVFCHGERGEGGHGGGKVLKDAVNADVVVQTVSEGRKDMPPFGAALTSEQIRD
                     VAAYVATMLPH"
     gene            complement(2463..3299)
                     /locus_tag="VM692_16960"
     CDS             complement(2463..3299)
                     /locus_tag="VM692_16960"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010446612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HUQ53921.1"
                     /translation="MARKKDPWGKTVLVAFDDGIAWVTLNRPDKRNAMSPTLNAEMQA
                     TLEALSIDPRCGVLVLTGAGDSFAAGMDLKEYFRETDGKPDMILKVRRTAEEWQWRRM
                     RDFPKPTIAMVNGWCFGGAFTPMIACDLAIAADEATFGLSEVNWGIIPAGNVTKATSE
                     TIGLRNALFYAMTGEPFQGKKAAEMGLVNESVPRKKLKKRTTEVARMLLTKNQVVLRG
                     IKIAMKRMRYMDWDASADYLYAKMGEALFMGASDNRANAMKAFLDDKTFKPGVQHFKG
                     TK"
     gene            3435..>4369
                     /locus_tag="VM692_16965"
     CDS             3435..>4369
                     /locus_tag="VM692_16965"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HUQ53922.1"
                     /translation="MAYDLLGKNFVPPDVHGKVTGKAKYAEDFRAEGMVFCKLLLSPM
                     PHARVRSVDTKAALALPGVVGILTADDLPPRPGPAEPILTNEPMWVGQPVLAVAAESE
                     QIAADAIDLIKIDFEQLPFTVDPLQSLYPGGPDARSDGNVIDNRLIGPPKLKQVKWSA
                     GDFARANEGQLPLGEPLVEWSYGDVEAGFAKAALVLDETFVTAGMSHHSMEPRSAMAY
                     WENGKCFVHGSTQSQSYVVPGLAGLIGITPDELVYIAETCGGGFGSKGTEYPVMAIPA
                     HMSKKVGRPVMLRITRAEEYYFGYARTGFQGRIRLG"
BASE COUNT          696 a         1447 c         1439 g          787 t
ORIGIN      
        1 aggattgcgc cggtggtcac gtcgagcgcg aacaaatcgc tggcgccggt cggcacgtag
       61 acgacgccgt cgatcacgag cggctgcgct tcgccggagt actgcggtcc gacgcccgag
      121 ccgccgagct gcgtgcgcca cacgcccttc aaggtcgcga cgttgccgcg atcgatttgc
      181 gtcagcggcg agtgccgctg gttgtaccaa ttgccgccgt tggtcggcca gctcgtcgtc
      241 ggcagcgcga cgagcgacgt gtcgggttgc cggcacgcgg cgagcagtgt gcaagaaagg
      301 gcgaggccga cgagcgttcc gcaacggcga tccatcgtca gcgtcctcag tgcggcaaca
      361 tcgtcgcgac gtaggcggcg acgtcgcgaa tctgctcgga agtcagcgcc gcgccgaagg
      421 gcggcatgtc cttgcggcct tcgctgaccg tctgcacgac gacgtcggcg ttgacggcgt
      481 ccttcagcac cttgccgccg ccgtggcccc cttcgcctcg ttcgccgtga cagaacacgc
      541 acgcggtgtc gtagacgatc ttgccggcgg ccgggtccgc ggtgccttcg gcctcgcgcg
      601 tgaacagcat cgcggcaccc ggtgatgttg caggcggcag cgtgccgtcc aagccgaaca
      661 gccagacgct atcgcccttc ggcgagcccg cgaacaagtt acccgccgag tacgcgacga
      721 cgtactgctt gcccgcgtgc tcgaacacgc tgacgggcgc gttcatgccg gcgcccgttt
      781 gaaactccca aagtctgacg ccgttcttcg aatcgagcgc cgtcagccgg ccgtcgttgc
      841 ggccgacgaa cgtgacgccg ccggccgtcg tcgtggagcc gctgtaacac gcgtccgccc
      901 actgttgttg ccagacgagc gtgttcgtgt gcatgtcgag cgcggcgaag acgccgaaat
      961 tcggcaggcc cgacgcgccg aagatgccgg cgacgtagcg ttcgccggcc ggcggccgct
     1021 cggtcgagag cgcatcggct ttgaacacgc cgacgcggtc ttgcgcgcac acatacaaat
     1081 aaccgctcgc ggggtcgtac gagctcggcg gccagttcgt gccgccgccg aacgacggct
     1141 tgaccggcac ttcacggtcg gtccagaacg gcgtgaaaat cttgccgccg ttgacgagcg
     1201 cgaagccctc gggcgcgact gcaatttgct gcgggacgaa cgagtcgccg cgcgggtacg
     1261 gctgcgtcgc ggctgtcgct tgccgtggct cttgcggcac gggccgctcg tcgatgccga
     1321 cgagcggcgt gccgttcgtg cggtcgagga tgtagaccca accggttttg ctcggctgcg
     1381 ctagacccaa gcgcatggcg ccgttcaact cgagatcgaa cagcacgacg gggctcgggc
     1441 cgtcgtagtc ccagatgtcg tgatgaactt gctggaagtg ccagcggtgc tggcccgtct
     1501 tcgcgtcgag cgcgacgatc gacgccgaga acaagttgtc gccggcacgc accccgccgt
     1561 tgtagtcggg gcccggattg cccgtcgaga agaaaatgag cccgagctcg ggatcgacgg
     1621 ccggcgtttg ccagacggac gcgccgccgt gcatccacag atcgttgtcc tgcggccagg
     1681 tctcgtgacc gagctcgccc gggcccggga tcgtgtagaa cgtccagacg agcttgccgt
     1741 ttttggcgtc gaacgctttg actcggccgc gaatgccgta ctcggcgccg gcgaagcccg
     1801 tgatgacgag gccgtcgtag tagagcggtg cgctcgtgat cgtgagcccg tcctgccaac
     1861 gctcggcttg cacttgccac gcgacggtgc cggtcttctg atcgagcgcg acgagcttgc
     1921 cgtcgagttg gccgacgaac accatgcctt gacctaagcc gacgccgcgg ctcgtccagc
     1981 cgcagcagac gacgtcgttg gccgcgtcga ggttcgcggc gtacgaccag aggattgcgc
     2041 cgctctcgac gcctatcgcg aacacgtcgt cggcgcccgt gacgatgtag atgacgccgt
     2101 cgtagacgat cggctgcgct tcgccggagt acttggtgcc gacgccggag cctcccaagc
     2161 gcgtgcgcca gacgcctttg aggttggcga cgttgtcgcg gtcgatctgc ttcagcggcg
     2221 aatagcgttg gttgtaccaa ttgcccccgt tcgtcggcca acccgccgtc ggcgctgcga
     2281 caagcgcgtc gcgtttgaac gccggcgcgg ggcgtatgcc ctgcgacgcc agcggtgccg
     2341 tgaggctcga ggcaacgatc gcaacgaccg cgaccagcgc ccgacgtact ctcgtcgagc
     2401 ggccgattcg aaccgacgtc tgcgtcatcg tgagctcccc ccgaagcttg tttggcccgc
     2461 ggctatttcg tgcccttgaa gtgctggact ccgggcttga acgttttgtc gtcgaggaac
     2521 gctttcatgg cgttcgcgcg gttgtcgctc gcgcccatga acaaggcctc gcccatcttg
     2581 gcgtacaagt aatcggccga ggcgtcccag tccatgtacc gcatgcgctt catcgcgatc
     2641 ttgatgccgc gcaacacgac ctgattcttc gtcagcaaca tgcgcgcgac ctccgtcgtc
     2701 cgcttcttga gcttcttgcg cggcaccgat tcgttgacga ggcccatctc ggcggccttc
     2761 ttaccttgga acggctcgcc cgtcatcgca tagaacagcg cgttgcgcag cccgatcgtc
     2821 tccgacgtcg ccttcgtcac gttgccggcc gggatgatgc cccagttgac ctcggacaga
     2881 ccgaacgtcg cttcatcggc agcgatcgcg aggtcgcacg cgatcatcgg cgtgaacgcg
     2941 ccgccgaagc accaaccgtt gaccatcgcg atggtcggct tcgggaagtc gcgcatgcgc
     3001 cgccactgcc actcctcggc cgtgcggcgc actttcaaaa tcatgtcggg cttgccgtcg
     3061 gtctcgcgga agtactcttt caagtccatg cccgcagcga acgaatcgcc ggcgcccgtc
     3121 agcacgagca cgccgcagcg cgggtcgatc gagagcgcct cgagcgtggc ttgcatctcg
     3181 gcgttcagcg tcgggctcat cgcgttgcgc ttatcggggc ggttcaacgt cacccaggcg
     3241 atgccgtcgt cgaaagcgac gagcacggtc ttgccccagg gatccttctt gcgggccatg
     3301 cggaatctcc tcaaagagtc gtggcggcgg cttttttcac tgttgttgtc tggttgccgg
     3361 aggtgccgct tgggcttaaa gtcctcgaat taccggacta ccaggttcgg ccggcagttt
     3421 atcaaggagt cgccatggct tacgacttgt tgggaaagaa cttcgtgccg ccagacgtgc
     3481 acggcaaagt gaccggcaag gccaaatacg cggaagactt ccgtgccgag ggcatggtgt
     3541 tttgcaagct gctgctgtcg ccgatgccgc atgcgcgcgt tcgcagcgtc gacacgaagg
     3601 ccgcgctcgc gctgcccggt gtcgtcggca ttctcacggc cgacgacctg ccgccgcgcc
     3661 ccggccccgc tgagccgatc ctgacgaacg agccgatgtg ggtcggccag ccggttctcg
     3721 ccgtcgcggc ggagtccgag cagatcgccg ccgatgcgat cgacctcatc aagatcgatt
     3781 tcgagcagct gcctttcacc gtcgacccgc tccagagctt gtacccgggc ggccccgacg
     3841 cgcgcagcga cggcaacgtc atcgataacc gcttgatcgg cccgccaaag ctgaaacagg
     3901 tcaagtggtc ggccggcgac ttcgcgcgcg ccaacgaagg tcagctgccg ctcggcgagc
     3961 cgctcgtcga atggtcgtac ggcgacgtcg aagcgggttt tgcgaaagcg gcgctcgtgc
     4021 tcgacgagac gttcgtgacg gccggcatgt cgcatcactc gatggagccg cgctcggcga
     4081 tggcctattg ggagaacggc aagtgtttcg tgcacggctc gacgcagagc cagagctacg
     4141 tcgtgccagg gctggccggc ttgatcggca tcacgccgga cgagctcgtc tacatcgccg
     4201 agacttgcgg cggcggtttc ggttcgaagg gcactgaata tcccgtgatg gcgatcccgg
     4261 cgcacatgtc gaagaaggtc ggccggcccg tgatgctgcg catcacgcgc gccgaggagt
     4321 actacttcgg ctacgcgcgc acgggctttc aaggccgcat ccgcttggg
//
LOCUS       DATLEE010000772         2922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8093716, whole genome shotgun sequence.
ACCESSION   DATLEE010000772 DATLEE010000000
VERSION     DATLEE010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8093716"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="VM692_16970"
     CDS             complement(<1..1475)
                     /locus_tag="VM692_16970"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HUQ53923.1"
                     /translation="MTEDRSLPRATLGALGVVYGDIGTSPLYALKETVAITGEDTASV
                     LGVLSLIFWSLLSVVTLKYIIVTLRSDNAGEGGTLSLLALVQQKFGTTGVWAARFVAL
                     AALGTAMFYCEALITPAVTVLSAVEGLSLIDPVFTNTVLPITLGIIVALFALQSRGTA
                     RVGGLFGPIMVLWFGTLAVLGLRGIAAAPGVLVAVSPTYAVALLTTHPSVALTILGAV
                     FLVLTGAEALYADMGHFGKTPVRLAWIGCVWPALLLSYFGQGALLISSDASIEHPLFE
                     LASPGLLPLFVALATAASAIASQATISGAFSVTRQAVQLDLLPRVKIRQTSADERGQI
                     YVPAANAAMFLAVVGFVVAFRSSSALTSAYGASVIGAMIITTILGAVVARNRWNWPLW
                     RVAALFGLFLLVDLAFLAGNVTKIAHGGWVPLTLASLMFAGFITWRDGRAKLRHELRE
                     RAVPWTELPNLLASCTRVPGTAVFLVSQSGFVPTALLRNIE"
     gene            1650..>2922
                     /locus_tag="VM692_16975"
     CDS             1650..>2922
                     /locus_tag="VM692_16975"
                     /EC_number="1.13.11.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallate dioxygenase"
                     /protein_id="HUQ53924.1"
                     /translation="MATIVGGIASSHTPTIGFAFDGGKQNDPVWAPIFAGYKPVQDWL
                     AAKKPDVLFLIYNDHVTSFFFDHYSHFALGVGEEFPVADEGGGPRALPAVKGHPKLAQ
                     HIAGCLVADEFDLSYFQNKGLDHGVFSPLSLLWPHDPTWPGAIVPLQVGVLQFPTTTA
                     RRCYKLGLSLRKAIESYPEDLKVAVVGTGGLSHQVQGERAGFNNTPWDLEFLELLIND
                     PEKLTTMTVAEYAKLGGMEGSEVIMWLIMRGALTKKVKKLHQTYYLPSMTPIATLILE
                     NDSSESTGESAESVRRRAARELDGVERLEGTYPFTLDRSVKAYRLNQFLHSLIQPERR
                     KQFLKDPEPLFEAAKLTEQERDMVRRRDWRALIQYGVIFFLLEKLGAVVGTTNLHIYA
                     GMRGQSLEDFQKTRNAQVLYSVAGKDEGKKDW"
BASE COUNT          599 a          925 c          960 g          438 t
ORIGIN      
        1 tgctcgatgt tgcgcagcag cgcggtcggc acgaagccgc tctgactgac gagaaacacg
       61 gccgtaccgg gcacgcgcgt gcagctcgcc agcagattcg gcagctccgt ccaaggtact
      121 gcgcgctcgc gcagctcgtg ccgcagcttc gcgcggccgt cgcgccaggt gatgaagccc
      181 gcgaacatca gcgacgccaa cgtcagtggc acccagccgc cgtgcgcgat cttcgtgacg
      241 ttgccggcga gaaatgcgag gtccacgagc aagaacagcc cgaacaaggc tgcgacgcgc
      301 cacaacggcc agttccagcg gttgcgtgcg acgacggcgc cgagaatcgt cgtgatgatc
      361 atggcgccga tgacggaagc gccatacgcc gacgtcaggg cgctcgacga gcggaacgca
      421 acgacgaagc cgacgacggc caagaacatg gcggcattgg ccgccggcac gtagatctga
      481 ccgcgctcgt ccgccgatgt ctggcgaatc tttacgcgcg gcaacagatc gagctgcacg
      541 gcctgacgcg tgaccgaaaa tgctcccgag atcgtcgcct gcgaggcgat cgcgctcgcg
      601 gccgtggcca gcgcgacgaa cagcggcaac aggcccggcg acgcgagctc gaacaacgga
      661 tgctcgatcg aggcatccga gctgatcaaa agcgcgccct ggccgaaata gctcagcaaa
      721 agcgctggcc acacgcagcc gatccatgcg agccggacgg gtgtcttacc gaaatgcccc
      781 atgtccgcgt agagcgcttc ggcgccggtc agcacgagga acacggcgcc caagatcgtc
      841 agcgcgacgc tggggtgcgt cgtaagcaag gccaccgcgt aagtcggcga aacggcgacg
      901 agcaccccgg gcgccgccgc gatcccgcgc aagccgagca cggcgagcgt gccgaaccac
      961 aacaccatga tcggaccgaa caggccgccg actctcgcgg tgccgcgact ctgcaaagcg
     1021 aacagcgcga cgatgatgcc gagcgtgatt ggcagcacgg tgttcgtgaa gacgggatcg
     1081 atcagcgaca gaccctcgac ggcgctgagc acggtcacgg ccggcgtaat cagcgcttcg
     1141 caatagaaca tcgcggtgcc gagcgccgcg agcgcgacga agcgcgccgc ccagacgccc
     1201 gttgtgccga acttctgctg cacgagagcg agcagcgaca gagtgccgcc ttcgccggcg
     1261 ttatcggacc gcagcgtcac gatgatgtac ttcagcgtaa cgaccgacag caggctccaa
     1321 aagatcaacg acaaaacgcc gagcaccgac gccgtgtctt cgccggtgat cgcgacggtt
     1381 tccttcagcg cataaagcgg gctcgtgccg atgtcgccat agacgacacc gagcgcgccg
     1441 agcgttgcac ggggcagaga gcgatcttcg gtcatcgatt ggctaaacgg cgggtatggc
     1501 gcgcagcgag tggggcgagt gcgagggcgg ggtggtcggc gggtgaggta gtcctgatgt
     1561 caagattcta cgcctccgcg ccggcgcggt cattgcgaga cgtactagaa tgcgaaccaa
     1621 gtacgacgac gctctgaggg aacagcagaa tggccacgat agtgggtgga atcgcgagct
     1681 cgcacacgcc gacgatcggc ttcgcgttcg acggcggcaa gcaaaacgac cccgtgtggg
     1741 cgccgatttt cgcgggctac aagcccgtgc aggattggct cgcggccaag aagcccgacg
     1801 tgctgttcct catttataac gaccacgtca cgtcgttctt cttcgatcac tactcgcact
     1861 tcgcactcgg cgtcggcgaa gagttcccgg tcgccgacga gggcggcggg ccgcgcgcgc
     1921 tgccggccgt caaagggcac ccgaagctcg cgcagcacat cgcggggtgt ctcgtcgccg
     1981 acgagttcga cttgtcgtac ttccagaaca aggggctcga ccacggcgtg ttctcgccgc
     2041 tgtcgctgct gtggccgcac gaccccacgt ggcccggcgc aatcgtgccg ctgcaagtcg
     2101 gcgtgctgca gtttccgacg acgacggcgc gacgctgcta caagctcggg ttgtcgctgc
     2161 gcaaggcaat cgagagctat ccagaggatc tcaaggtcgc cgtcgtcggc acgggcggtt
     2221 tatcgcacca agtgcaaggc gagcgcgcgg gcttcaacaa cacgccgtgg gatctcgagt
     2281 tcctcgagtt gctgatcaac gatcccgaga agctcacgac gatgacggtc gccgagtacg
     2341 cgaagctcgg cggcatggaa ggctccgaag tcatcatgtg gctaatcatg cgcggcgcgc
     2401 tgacgaagaa ggtcaagaag ctgcaccaga cgtattacct gccgtccatg acgccgatcg
     2461 cgacgctgat cctcgaaaac gattcgagcg agtcgacggg cgagtcggct gaatccgtgc
     2521 gccgacgcgc ggcgcgcgag ctcgacggcg tcgaacggct cgaaggcacg tacccgttca
     2581 cgctcgatcg cagcgtaaag gcgtatcggc tgaaccagtt cttgcattcg ctgatccaac
     2641 ccgagcgccg caagcaattc ctcaaagatc ccgagccgct gttcgaggcg gcaaagctga
     2701 cggagcaaga gcgcgacatg gtgcggcggc gcgattggcg cgcgttgatc cagtacggcg
     2761 tgatcttttt cttgctcgag aagctcggtg ccgtcgtcgg cacgacgaac ctgcacatct
     2821 acgccggaat gcgcgggcaa tcgctcgagg atttccaaaa gacgcgcaac gcgcaggttt
     2881 tgtactcggt tgccggcaaa gacgaaggta agaaggactg gg
//
LOCUS       DATLEE010000773         6053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8107455, whole genome shotgun sequence.
ACCESSION   DATLEE010000773 DATLEE010000000
VERSION     DATLEE010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8107455"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1322
                     /locus_tag="VM692_16980"
     CDS             153..1322
                     /locus_tag="VM692_16980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUQ53925.1"
                     /translation="MAVTATAIEGEPRLRIMVLGLRGIIDVQGGIETHARRLYPLLAR
                     LGCDVEIVQRGAYYPRDRRPREWRRVALTYLWSPRTRIVETAVHTLLGVLYAAIKRPD
                     VLHLHAVGPGLLAPLARLFGLRVVVTHHGADYEREKWGPFAKAVLRTGERLGIGFAHR
                     PIVVSPVLKQTVETRYHIGATLIPNGAPLATPRATYGTLRRFGLERGRYVLCVARLDP
                     GKRQHDLIAAFQQARPEGWKLVLVGAIESGDAYSDQLVAAAARDPTVVLTGYQSGVAL
                     HELYSHAGLFVLPSAGEGHPIALLEAAVYEVPLLASAIPANLALPLPRDQYFPVGDTQ
                     ALARKLQTMTNGRAASSGERRALHATIRADYSWRKAAELTSSVYRCVARGGWQHG"
     gene            1315..3057
                     /locus_tag="VM692_16985"
     CDS             1315..3057
                     /locus_tag="VM692_16985"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HUQ53926.1"
                     /translation="MVRAVLVVPRYPGQPNRLDAYCEALNRKLAGDNIDPRPATLVRS
                     SGVSAALLNPSAAAQVQGASIAVGTLLAPRDDWHVPKAALPDGSFALLRADDARVELA
                     SDSVGSRTLWYAMTDRELIASTSQRAIVTLLGSFEPNRAALPWMLSSGTLGPTAAWDA
                     RVQRLQPGQRLLLDRASWRLESRTEPVVFEADESRPRAAHLERLLGAVSDACRRWSFD
                     ARKWVLTLSGGADSRSLLCLLRERGIATVTWGLPQAPEEEGNDAQIARTLAQTLCVPH
                     RFFAIDDRGDSVNALLDRFLSAGEGRVARISGYVDGFRVWKTLFDEGYHGVIRGDEAF
                     GSVPVRNAYSARWAASLTTLTDYFPDEEIETFELPEQSLPEALLRRDNETLATWRDRL
                     YQQSRVPTLLAGLTDLKTAYVDVGNPLLATSVLECVRALPDDLRTEKRLWRDVVDQQL
                     PNVAMATQVAIPSLTDFLMDPRVVDALLEELCSEAAASVLAPLLRARCCSALRAALRE
                     APPPGRNGERQRATGFARVVPAKLRAVMRHWSRFDLPPVVLAFRAYVATRMHRMLRLD
                     AATPPADQQPAVNA"
     gene            3070..4830
                     /locus_tag="VM692_16990"
     CDS             3070..4830
                     /locus_tag="VM692_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53927.1"
                     /translation="MVRAVLVAPRHQGQPNRLDLYCDVLNRKLAGSNIEPRPPLFARR
                     AGLSAAMLNPNGAARMHGASIAVGALLGAGADWHVPRAPLPDGSFALLRGDESYVELV
                     ADSTASRTLWYALTDDELIVSSSQRAIVTLLGSFEPNRAALPWMLSSGTLGPTGGWDA
                     RLRRVQPGERVLLDRARWRLRSTVDAPSFVADRSLSRAEHLARLQTSVAAACDAWSFD
                     AKKWVLTLSGGADSRSLLCMLRDRGLATVTWGLSHTGEQAGNDALIAREVARALAVPH
                     RFLAIDAPGDPPDVVLDRFLAVGEGRVDRISGYVDGFCVWKTLFDEGYEGVVRGDEAF
                     GWVPAHTSYAVRSATNLTTLADFFPANELETFELPTQRLPDSLLRGSDETLATWRDRL
                     YQQSRVPTFLAALTDLKTAYLEVGTPLLARSVLDCVRAMPDELRTRKRLWMELVSAQL
                     PQVPFARRVAIPSVTDFLTDSRVLTLLLDELKSERAAELFAAPLRTRLCTGLATPRNN
                     AQAAKRDRWRDSALARVLPAPLKAAVRNWRANRPSIEPLVLAFRALVAARMHGVLAAD
                     AATPAPETTVDTLRARYRAV"
     gene            complement(4844..5698)
                     /locus_tag="VM692_16995"
     CDS             complement(4844..5698)
                     /locus_tag="VM692_16995"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HUQ53928.1"
                     /translation="MSNLEKGLKRARAALHSIGPGAADLAPILDTGNVAVAPAPAPAL
                     GPTGEPAFDIERLEWVQPDVDLLEQNRIVIDERSSASAAYKVLRTRVLQRMRRNGWKT
                     LAVTGTSPNEGKTLTAINLSINLAWHLTTSVVLVDMDLRNPSVHRYLGIDTRYGVMDY
                     LNGDVPLVRAGVRPGIERLGVIMNDRPVANASELLSSPEAVQLIEEVKRGDDRIAIFD
                     MPPVFAGDDVLAFAPLVDAVLIVLAQGTTNRASLVALKELLQNVNVIGSVLNRSSERV
                     APYYYGRY"
     gene            complement(5709..>6053)
                     /locus_tag="VM692_17000"
     CDS             complement(5709..>6053)
                     /locus_tag="VM692_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53929.1"
                     /translation="LNLEEDPNAERFTVLEGPSVASSPSSPNRFAVLVLSLAIGVALG
                     AAAVTIAERSDQTVRNAQDVVAFLEVPPLVAIPYVENRADLKRNARRRLLAATAVSLW
                     LGAVFLLVVTPL"
BASE COUNT          887 a         2080 c         2096 g          990 t
ORIGIN      
        1 gggctcgccg cgacgttgcg ccgcgtcgcc gccctgcccg atgcggaagt taccgccgtc
       61 ggcatggctg cgcgccgccg catcgaagac gaattttcgc cgcagcgcta tctcgagcgg
      121 attcgaggcg tgtacggccg attgggggtc gcatggccgt gacggcgact gcaatcgagg
      181 gcgagccgcg tctgcgcatc atggtgctcg ggctgcgcgg catcatcgat gtccaaggcg
      241 gcatcgagac gcacgcacgc cggctctacc cgctgctcgc gcggctcggc tgcgacgtcg
      301 agatcgtgca gcgcggcgcg tactacccgc gcgaccggcg gccgcgagag tggcgccggg
      361 tcgcgctgac gtatctctgg agcccgcgga cgcgcatcgt cgagacggcc gtgcacacgc
      421 tgctcggcgt tctgtacgcc gcgatcaaac ggcccgacgt gctgcatctg cacgccgtcg
      481 gcccgggtct gttggcaccg ctcgcacgcc tgttcgggct gcgcgtcgtc gtgacgcacc
      541 acggcgccga ctatgagcgc gagaagtggg gcccatttgc gaaggcggtg ctgcggaccg
      601 gcgagcggct cggcatcggt ttcgcgcacc ggccgatcgt cgtgtcgccc gtgctcaagc
      661 aaaccgtcga gacgcgctat cacatcggcg cgaccttgat tccgaacggc gcgccgctcg
      721 cgacgccgcg agcaacgtac ggcacgctgc gccgcttcgg cttggagcgc ggccgctacg
      781 tgctctgcgt cgcgcgcttg gatcccggca aacgccagca cgacttgatt gcggcgtttc
      841 agcaagctcg gcctgagggc tggaagctcg tgctcgtcgg cgcgatcgag tcgggcgacg
      901 cctattccga ccagctcgtc gcggcggccg cgcgcgaccc gacagtcgtg ctgacgggct
      961 atcagagcgg cgtcgcgctg cacgagctgt attcacacgc cggcctgttc gtgctgccgt
     1021 cggcgggcga aggccacccg atcgcgctgc tcgaagcggc cgtctacgag gtgccgctgc
     1081 tcgcgagcgc gatcccggcg aacttggcgc tgccgttgcc tcgcgaccag tacttccccg
     1141 tcggggacac gcaagcgctg gcacggaaac ttcaaacaat gacgaacggc cgcgcggcat
     1201 cgtccggcga gcgacgcgcg ctgcacgcga ccattcgtgc ggactattca tggcgcaaag
     1261 ccgccgagct cacgagctcg gtttaccgct gtgtcgctcg gggagggtgg caacatggtt
     1321 agggcagttc tcgtcgtacc gcgatatccg ggccagccga atcggctcga tgcctactgc
     1381 gaggcgttga atcggaagtt ggccggcgac aacatcgatc cgcggccggc aacgctcgtg
     1441 cgtagctcgg gggtctcggc tgcactgttg aaccccagcg ccgcggcgca agtgcaaggc
     1501 gccagcattg cagtcggcac cctgctcgcg ccacgcgacg attggcacgt gccgaaggcg
     1561 gctctacctg acggcagctt cgcgctgctg cgcgcggacg acgcgcgcgt tgagctcgcc
     1621 tccgacagcg tcgggtcgcg gacgctctgg tatgcgatga cggaccgcga gctgatcgct
     1681 tcgacgtcgc agcgcgcgat cgtgacgctg ctgggatcgt tcgagccgaa ccgcgccgcc
     1741 ctgccgtgga tgctctcgtc ggggacgctg gggccgacgg ccgcctggga cgcgcgtgtc
     1801 cagcggctgc aaccgggcca gcgcttgcta ctcgatcgcg cgagctggcg cctcgagtcg
     1861 cgcacggaac ccgtcgtgtt cgaggccgac gagtcgaggc cgcgcgccgc acacctcgag
     1921 cgcttgctcg gcgccgtttc cgacgcctgc cgacgctggt cgttcgatgc gcgcaaatgg
     1981 gtgctgacgt tgtccggcgg cgcggacagc cgcagcctgc tgtgcctgtt acgcgagcgc
     2041 ggcatcgcga ccgtcacgtg gggactgccg caagcccccg aggaggaagg caacgacgcg
     2101 caaatcgccc gcacgctcgc gcagacgctg tgcgtgccgc atcgcttctt cgccatcgac
     2161 gaccgcggcg attccgtcaa cgcgctgctc gatcgttttt tgagcgcggg cgaaggccgc
     2221 gtcgcacgga tctccggcta cgtcgacggg tttcgcgtct ggaagacttt gttcgacgag
     2281 ggctatcacg gcgtgattcg cggcgacgag gcattcggct ccgtgcccgt gcgcaacgcg
     2341 tacagcgccc gctgggcggc gagcctgaca acgctgacgg actattttcc ggacgaggaa
     2401 atcgagacgt tcgagctgcc cgagcagtcg ctgcccgagg cgctcctgcg ccgcgacaac
     2461 gagacgctcg caacttggcg cgaccgcctc tatcagcaat cgcgcgtgcc gactctgctc
     2521 gcgggactca cggacttgaa gacggcctac gtcgacgtcg gcaacccgct gctcgcaacg
     2581 tcggtgctcg aatgcgtgcg tgcgctgccc gacgacctgc gcaccgagaa gcgcctctgg
     2641 cgcgacgtcg tcgaccagca gctgccgaac gtcgcgatgg caacccaggt tgcgatccca
     2701 tcgctgaccg atttcctcat ggatccacgc gtcgtggacg cgctgctcga ggagctgtgc
     2761 tcagaagccg cggcctccgt gctggcgccg ttgctgcgcg cgcgctgctg cagcgcgttg
     2821 cgcgcggcct tgcgcgaggc gccgcccccg ggccgcaacg gcgagcgaca gcgagcgacc
     2881 ggcttcgcgc gagtcgtgcc ggccaagctg cgcgccgtca tgcgccactg gtcgcggttc
     2941 gatctgccgc cggtcgtgct cgcgttccgc gcctacgtcg cgacgcgcat gcatcggatg
     3001 ctccggctcg acgcggcaac gccgcccgcc gatcaacaac ccgcggtcaa cgcctgatgg
     3061 gtgctcaaca tggttagggc cgtcctcgtc gcgccgcgcc atcaaggcca gccgaatcgg
     3121 ctcgacctgt actgcgacgt gctgaatcgc aagctcgcgg gaagcaacat cgagccgcgg
     3181 ccgccgctgt ttgctcggcg cgcgggcctg tcggccgcga tgttgaaccc gaacggcgcg
     3241 gcacgcatgc acggcgccag catcgccgtc ggtgcgctgc tcggcgccgg cgccgactgg
     3301 cacgtgccgc gtgccccgct gccggacggc agcttcgctc tgctgcgcgg cgacgagtcc
     3361 tacgtcgagc tcgtcgccga cagcactgcg tcgcgcacgc tgtggtacgc actgacggac
     3421 gacgagctca tcgtctcgag ctcgcaacgt gcgatcgtga cgttgctcgg ctcgttcgag
     3481 ccgaatcgtg cggcgctgcc ttggatgctg tcgtccggca cgttggggcc gaccggcggt
     3541 tgggacgcgc gcttgaggcg cgtgcaaccc ggcgaacgcg tgctgctcga tcgtgcgcgc
     3601 tggcgcttac gctcgacagt cgacgcgccc tccttcgtcg cggaccgcag cctgtcgcga
     3661 gccgagcatc tcgcgcgcct gcaaacttcc gtcgccgcag cgtgcgacgc gtggtcgttc
     3721 gacgcgaaaa agtgggtgct gacgttgtcg ggcggggcgg acagtcggag cctgttgtgc
     3781 atgctgcgcg accgcggcct cgcaaccgtg acgtggggct tgtcgcacac gggcgagcaa
     3841 gccggtaacg atgcgctgat cgcgcgcgag gtcgctcgcg cgctggccgt gccgcatcgg
     3901 ttcctcgcga tcgacgcgcc cggcgaccct cccgatgtcg tcctcgaccg gtttctcgcc
     3961 gtcggcgaag gccgcgtgga tcgcatctcg ggctacgtgg acggcttctg cgtctggaag
     4021 acgttgttcg acgaggggta cgagggcgtc gtgcgcggcg acgaggcgtt cggctgggtg
     4081 cctgctcata cgtcgtacgc cgtgcgctcg gcgacgaatc tcacgacgct cgcggacttc
     4141 ttcccggcaa acgagctcga gacgttcgag ctgccgacgc agcggctacc ggactcgttg
     4201 ctgcgcggca gcgacgagac gctcgcgacg tggcgcgata ggctctatca gcagtcgcgt
     4261 gtgccgacgt tccttgcggc gctgacggac ttgaagaccg cgtacctcga ggtcggcacg
     4321 ccgctgctgg cgcgctcggt gctcgactgc gtgcgcgcga tgcctgacga gctgcgcacg
     4381 cggaaacgcc tctggatgga gctcgtcagc gcacagctcc cgcaagtgcc gttcgctcgc
     4441 cgcgttgcga ttccgtccgt aacggacttc ctgaccgact ctcgtgtgct gacgctgctg
     4501 ctcgacgagc tcaagtccga gcgcgcggcc gagctgttcg cggcgccgct gcgcactcgc
     4561 ctgtgcacgg ggctcgcgac gccgcggaac aacgcgcagg ccgccaagcg cgaccggtgg
     4621 cgcgactccg ccctcgcgcg cgtgctgccg gcaccgctga aggcggccgt gcgcaactgg
     4681 cgcgccaacc gcccctcgat cgagccgctc gtgctggcgt tccgcgcatt ggtcgccgcg
     4741 cgcatgcacg gcgtcctcgc cgcggacgcg gcaacgcccg ccccggaaac cacggtcgat
     4801 accttaaggg cgcgttaccg cgcggtctga gagctggccg ccgtcagtag cggccgtagt
     4861 agtaaggcgc cacgcgctcc gaggagcgat ttagcacgct gccgatcacg ttgacgtttt
     4921 gcagcagctc tttgagcgcg acgaggcttg cgcgattcgt ggtgccctga gccagcacga
     4981 tcaacacggc gtcgacgagc ggcgcgaacg cgagcacgtc gtcgccggcg aacaccggcg
     5041 gcatgtcgaa gatcgcgatg cggtcgtcgc cgcgcttcac ctcttcgatg agctgaacgg
     5101 cttcgggcga ggacaagagc tccgaggcgt tggccaccgg ccgatcgttc atgatcacgc
     5161 cgagccgctc gatgcctgga cggacgccgg cgcgcacgag cggcacgtcg ccgttcaagt
     5221 agtccatgac gccgtagcgc gtgtcgatgc cgagatagcg gtggaccgac gggttgcgca
     5281 aatccatgtc gacgagcacg accgaagtcg tcaggtgcca cgcgagattg atgctcaggt
     5341 tgatggccgt cagcgtcttg ccttcgttgg gggacgttcc cgtcacggca agcgtcttcc
     5401 aaccgttgcg gcgcattctc tgcagcacgc gcgtgcgtag aaccttgtag gccgcgctgg
     5461 cggacgagcg ttcgtcgatg acgatgcggt tctgctcgag caagtcgacg tcgggctgta
     5521 cccattcgag gcgctcgatg tcgaacgccg gctcgcccgt cggtcccagt gcgggagccg
     5581 gtgcaggcgc gaccgccacg ttgcccgtgt ccaatatcgg cgcgaggtcc gccgcaccgg
     5641 ggccgatgga atgtaacgcg gctcgcgccc gcttgagacc cttctcgagg ttcgacattg
     5701 cgagactcct aaagcggtgt gacgaccaac aggaacaccg ccccgagcca caagctcacg
     5761 gcggtagcag ccaagaggcg ccggcgcgca ttgcgcttca aatcggcgcg gttctcgacg
     5821 tacgggatcg cgacgagcgg cggcacctcg aggaatgcga ccacgtcttg tgcattgcgg
     5881 accgtttgat cggaccgctc ggcgatcgta acggcggcag cgccgagcgc gacgccgatc
     5941 gccagggata gcacgagcac ggcgaagcgg ttcgggctcg acgggctcga cgccaccgac
     6001 ggcccttcga ggaccgtgaa gcgctcggcg ttcgggtctt cctcgagatt cag
//
LOCUS       DATLEE010000774         4640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8107941, whole genome shotgun sequence.
ACCESSION   DATLEE010000774 DATLEE010000000
VERSION     DATLEE010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8107941"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VM692_17005"
     CDS             <1..254
                     /locus_tag="VM692_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53930.1"
                     /translation="PSPWCYARVGLDHYTMRLLGQASRFATRKLKWRKLGAALLQPFR
                     RFNEGYSWYGSRNHRSTLYSWAAMELVGKLRERGVSVPM"
     gene            complement(270..1781)
                     /locus_tag="VM692_17010"
     CDS             complement(270..1781)
                     /locus_tag="VM692_17010"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HUQ53931.1"
                     /translation="MLSTNALVLGSVAIGFASSVLIAALFGLSRRLDAYYAALMVPNL
                     FVTLCLDYLGKNFLPVYARARAQSAQTASEVTSSVVTIVGLLAAALALALCLASHHVF
                     RALLPGFDAADLGLVSRYFWIMAPAMVLTAVTCFHEYVCQHDEQYTRIAAITASQPVV
                     NFIAIAGAGPIIGEYALPIGYVAGQAITFLLMTRYARYRYRPRFTVRSEWEKPIFTNT
                     AVVMGSGLLVRTRTLITNFLASFVGDGAIAALNFGYRLIEPLERTTFSGVRRLMFSRT
                     ARLVIEDNSRELARLYRLALTASFLLLTPLLWWVVLERELVIGLLFERGAFNATMTAL
                     VAVTLIGYAPSVTFAGVNSILSNAFYAMSRIAVPALVMPIGTLLYLIVALAAYRPLGI
                     LGLALAPTTMHATVFVLLLFMLSKHMPQLEVWSVLGRIAAYVLLGGAALGLPQLLLAP
                     LGLPKLVEAGLTLSVGVTLYFGVLAMARERTFLDVVSYFRRAHPLLDRRPAAT"
     gene            2045..3841
                     /locus_tag="VM692_17015"
     CDS             2045..3841
                     /locus_tag="VM692_17015"
                     /inference="COORDINATES: protein motif:HMM:NF024927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53932.1"
                     /translation="MNAALRALTHFETYRSRAAAIDARIACGQVWRDADRSERDWAVD
                     ATTGVALLVNGAVFSVGPIARRIDARRLLEGYLTAGALAPRELDGAFQILLADPRKGS
                     VFLYNDRLGTLPAYYGRHNGVVCVAPEAKAVFAALSLQPQLATAGIVSFLSCGYCLGS
                     TTLFAGVSFLEPGSSLEIAVASGEIAVQRYWKMQYRSARALRSRAAAESALHGAIARA
                     HEVLVGDSERGFDLMLSGGWDSRGILAYLHSLGRMPSTGVSWGLTKDIPQSDPFIASQ
                     LAQRFAVPFKFVSYDSDEFVANARNWCYLSELANDNIGWYAEGASVLAGQYHTAADFT
                     LVGDESWGWHGHPRTEQDARNACMPASPGAQTLACLSPGIRDESRARYEADVTRVLAA
                     CDNEHPADRRDFLYLHGRVARFIFALGYYKEFATEVRRPFLLGNVLDVLAEVPARFRA
                     DKNLYISMLGRFFPEVAAFPSRAADSLPDWTRDIRAKPELRNLFLDLLDERSMGGTLG
                     TVLDGASVEKLKNTFFAGNAASKPAAGKRSLVARLPLRMRQRLRASGLYPGSTNMAGA
                     YASRGAATLVRCIALLSLLQRSLPAFAARAAP"
     gene            complement(3838..>4640)
                     /locus_tag="VM692_17020"
     CDS             complement(3838..>4640)
                     /locus_tag="VM692_17020"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ53933.1"
                     /translation="RYVVYSHSHFDHAEGGAVFADTATFVAHENMLRNMDGRYPQMPG
                     DMIDRNDNGVIDRDEIDIPTNTRPGVCGMGPGFFDGIDRNSDGVATPQELQQYIVKPT
                     IVYNDRMRIELGGKVVELVHPGLNHSNDATVMVFPAERTAFATEFIADALVAGNPRSL
                     PSACGPFDGSPLAEWIKSYRTVEDLDDVDVIVTGHGPNLVDKALVRDTREFFERLRAD
                     VAAGMAAGQTLEQLKQSVLLEPYEDWANYERLRANNVEAAYRNLLMYR"
BASE COUNT          800 a         1472 c         1568 g          800 t
ORIGIN      
        1 tgccgtcgcc gtggtgttac gcgcgagtcg ggctcgatca ttacacgatg cggctgttgg
       61 gccaggcctc tcgcttcgcg acgcgcaaac tgaagtggcg caagctcggc gcagcgctgc
      121 ttcagccgtt tcggcgcttc aacgaaggct actcctggta cggcagccgc aatcaccgca
      181 gcacgctgta ttcatgggcg gcgatggagc tcgtcggcaa gctgcgcgag cgcggcgtca
      241 gcgtgccgat gtagcgataa cgcctcggct caagtggccg ccggccgcct gtcgagcaac
      301 ggatgcgcgc gccggaagta cgacacgacg tccaagaagg tgcgctcgcg cgccatcgcg
      361 agcacgccga aatacagtgt tacgccgacg gatagcgtca gccccgcttc gacgagcttg
      421 ggcaagccga gcggtgcgag cagcagctgc ggcaacccga gagccgcgcc gccaagcagc
      481 acgtacgcgg caatacgccc gagcacggac caaacttcga gctgcggcat gtgcttcgag
      541 agcatgaaca gcagcaagac gaacacggtc gcgtgcatcg tcgtcggcgc cagcgcgagg
      601 ccaaggatgc cgagcggccg ataagccgcc agcgcgacga tgagatagag cagcgtgccg
      661 atcggcatca cgagcgcagg gacggcgatg cggctcatcg cgtagaacgc gttcgacaag
      721 atcgagttga cgcccgcgaa cgtgaccgac ggtgcatagc cgatcagcgt gacggccacg
      781 agcgccgtca tcgtggcatt gaatgcgccg cgctcgaaca aaagcccgat cacgagctcc
      841 ctctcgagca cgacccacca caacaacggc gtcagcagca agaagctcgc ggtcaacgcg
      901 agccggtaga gccgcgcgag ctcgcgcgag ttgtcttcga tcactagacg cgcagtgcgg
      961 ctgaacatca atcgccgcac gccggagaac gtcgttcgct caagcggctc gatgagcctg
     1021 taaccgaagt tcaacgctgc gatcgcgccg tcgccgacga acgacgccaa gaaattcgtg
     1081 atcagcgtgc gcgtgcgcac gagcaagccg ctgcccatca cgacggccgt gttcgtgaag
     1141 atcggctttt cccattcgct gcgtaccgtg aagcgcggcc ggtagcgata gcgcgcgtag
     1201 cgagtcatca gcaggaacgt gatcgcctgg ccggccacat agccgatagg caacgcgtat
     1261 tcgccgatga tggggccggc tcccgctatc gcgatgaagt tgacgacggg ctgcgaggcc
     1321 gtgatcgccg ctatgcgcgt gtactgctcg tcgtgctggc atacgtactc gtgaaagcac
     1381 gtgacggcgg tcaagaccat cgccggtgcc atgatccaga agtagcggct cacgagccca
     1441 aggtcggcag catcgaagcc cggcaacagc gcgcggaaga cgtggtgact tgcgaggcac
     1501 aacgcgagcg cgagcgcggc cgcaagcagg ccgacgatcg tgacgacgga ggaggtgact
     1561 tcggaagcgg tctgcgcact ctgggctcgc gcacgggcgt acaccggcag aaaattcttg
     1621 ccgagatagt cgagacacag cgtcacgaac aagttgggca ccatcagcgc ggcgtaatac
     1681 gcatcgagcc gccgcgacag gccgaacagc gctgcgatga ggacgctgct ggcgaagccg
     1741 atggcaacgg agccgaggac cagtgcgttc gtcgacagca tgctgcgcgc gctcgagcgc
     1801 gcgggttcgc tcgccgctga ggatttagcg gagcgcattg gttgtctttt ccaaaatttg
     1861 gaacgcggtc gcggatgttg cgggtgtcgc gcacagtaga gcgtgcgctt ctcgattcgc
     1921 ttcacacggc aaggtggtgc tctcggtgtg taaacgatga gagactgtgc aaaaggcgag
     1981 ccgacgggtg gtgatggtgc cgggggtggt gggagttgtt ggcgaacatg ccaacgtcga
     2041 gcgcgtgaac gccgcgctgc gcgcgctgac tcacttcgaa acttatcgtt cgcgagccgc
     2101 tgcgatcgac gcgcgtatcg cgtgcggcca agtctggcgc gacgcggatc gctccgagcg
     2161 cgattgggcc gtcgatgcta cgacgggcgt cgcgttgctc gtgaacggcg ccgtgttcag
     2221 cgttgggccg atagcgcgcc gaatcgacgc gcgccggttg ctcgagggtt acttgacggc
     2281 gggcgcgctc gcgccgcgcg agctcgacgg cgcgttccag atcctgctcg cggatccccg
     2341 caaggggagc gtctttctct acaacgaccg cctcggcaca ttgcccgcct actatgggcg
     2401 ccataacggc gtcgtctgcg tcgcgccgga ggccaaagcc gtgttcgcgg cgctctcgtt
     2461 gcagccgcag ttggcgacgg ccggcatcgt gagcttcttg agctgcggct attgccttgg
     2521 ctcgacgacg ctgttcgcgg gcgtgtcgtt cctcgagccc ggctcttcgc tcgaaatcgc
     2581 agtcgcgtcc ggcgagatcg ccgtgcagcg ctattggaag atgcaatacc ggtccgcgcg
     2641 tgcgctgcgt tcgcgcgccg ctgccgagag cgcactgcac ggcgcgattg cgcgtgcgca
     2701 cgaagtgctc gttggcgaca gcgagcgcgg cttcgacttg atgctgtcgg gcggctggga
     2761 ttcgcgcgga atactcgcgt atctgcactc gctcggccgc atgccgagca cgggagtcag
     2821 ctggggtctt acgaaggaca taccgcagtc ggatccgttc atcgcgtcgc agttggcgca
     2881 gcgtttcgca gtgccgttca agttcgtcag ctacgacagc gatgagttcg tcgccaacgc
     2941 ccgcaactgg tgctatctgt ccgagctcgc caacgacaac atcggctggt acgccgaagg
     3001 cgcgagcgtg ctggcgggcc agtaccacac ggccgccgat ttcaccttgg tcggcgacga
     3061 gtcatggggc tggcacggac atccgcgcac ggagcaggac gctcgtaacg cgtgcatgcc
     3121 ggcatcgccg ggcgcgcaga ctttggcttg cttgtcgccc ggtatccgcg acgagtcgcg
     3181 cgcccgttat gaggccgacg tcactcgcgt gcttgccgct tgcgacaacg agcacccggc
     3241 cgatcggcgc gactttctgt acttgcatgg ccgcgtagcg cgcttcattt tcgcgctcgg
     3301 gtactacaag gaattcgcga ccgaggttcg gcggccgttc ctgctcggca acgtgctgga
     3361 cgtcttggcc gaggtgccgg cgcggttccg tgcagacaag aatctctaca tctcgatgct
     3421 cggccgcttc tttcccgagg tcgcggcgtt cccgtcgcga gccgcggaca gcttgcccga
     3481 ttggacgcgc gacatccgcg ccaaacccga gctgcgcaat ttgttcctcg atctgctcga
     3541 tgagcggagc atgggcggaa cgctcggcac ggtgctcgac ggcgcgtcgg tcgagaagtt
     3601 gaagaacacg ttcttcgccg gcaacgccgc aagcaagccg gccgccggca agagatcgtt
     3661 ggtcgcgcgc ctgccgctgc gcatgcggca acgactgcga gcgagcggcc tttatcccgg
     3721 cagcacgaac atggcagggg cctatgcgtc gcgcggcgcg gcaacgttgg tgcgctgcat
     3781 cgcgttgttg tcgctgctgc agcgctcgct gcccgccttc gcggcgcgcg cagcgcctta
     3841 gcgatacatc agcagattgc ggtaggcggc ttcgacgttg ttcgcgcgca gccgctcgta
     3901 gttcgcccag tcctcataag gctcgagcaa cacgctctgc ttgagctgct cgagcgtctg
     3961 gccggccgcc atgccggcgg cgacgtcggc tcgcagccgc tcgaagaact cgcgcgtgtc
     4021 gcgcacgagc gccttgtcga cgagattcgg gccgtggccc gtgactatca cgtcgacgtc
     4081 gtcgaggtcc tcgaccgtgc ggtacgactt gatccactcc gcgagcgggc tgccgtcgaa
     4141 cgggccgcac gcgctcggca acgagcgcgg attgccggcg acgagcgcgt cggcgatgaa
     4201 ctccgtcgcg aacgcggtgc gctcggcggg gaacaccatg accgtcgcgt cattcgagtg
     4261 gttgagcccc gggtgcacga gctcgacgac cttgccgccg agctcgatac gcatgcggtc
     4321 gttgtagacg atcgtcggct tgacgatgta ttgctgcagc tcttgcggcg tcgcgacacc
     4381 gtcgctgttg cggtcgatcc cgtcgaagaa gccgggcccc atgccgcaga ctccgggccg
     4441 cgtgttcgtc gggatgtcga tctcgtcgcg gtcgatgacg ccgttgtcgt tgcggtcgat
     4501 catgtcgccc ggcatctgcg gatagcggcc gtccatgttc cgcagcatgt tttcatgggc
     4561 aacgaacgtc gcggtgtcag cgaacaccgc cccgccctcg gcatgatcga agtggctgtg
     4621 gctgtagacc acatagcgga
//
LOCUS       DATLEE010000775         3481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8114390, whole genome shotgun sequence.
ACCESSION   DATLEE010000775 DATLEE010000000
VERSION     DATLEE010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8114390"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VM692_17025"
     CDS             complement(<1..783)
                     /locus_tag="VM692_17025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUQ53934.1"
                     /translation="MERSRLEPLGVPHSDYGLEAAEASRHEPSGLDRWLVKQITSKLG
                     ASPVRISLWDEPDVAPREGTVRMRVLDSKALWQLVINPDLHFGDLYSSGRLQVRGDLL
                     TLLLECYRYTAENTAIGHVLARGRKLAPDLHDSKRNIHHHYDLGNDFYKLWLDREALQ
                     YTCAYFADPAMTIEQAQQAKMHHVCRKLQLKAGESVVEAGGGWGGFALFMAKNYGVRV
                     RSFNISKEQIAHSRAWAKQQGLDGQVEFVEDDYRNLTGTFDAF"
     gene            1014..2072
                     /gene="moaA"
                     /locus_tag="VM692_17030"
     CDS             1014..2072
                     /gene="moaA"
                     /locus_tag="VM692_17030"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007396428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HUQ53935.1"
                     /translation="MTHTVTQWQTQAAVKDRLSRPLRDLRISVIDRCNYRCPYCMPAE
                     LFGENHTFLPRAHWLTPGEIKRVASVFLQLGVKKFRLTGGEPLLRRDIVEIVAGLAEL
                     HGAEDLAITTNGSRLGEHAAALKQAGLKRITVSLDSLDAEVFKEMNGGRGELEVVLRG
                     IEAARAEGFPIKLNTVVERGKNDHTLLELVDRFRGTGVIVRFIEYMDVGTVNGWQPRQ
                     VVPSKELLARLRERWTLSPLDSNYRGEVAKRYSFDDGQGEIGFISSVSEPFCGDCHRA
                     RLSADGTVYTCLFAASGTELRTPLRSGASDADLVGLLQNVWRNRTDRYSEIRGRLLEH
                     KPEGVEKPERVEMYRMGG"
     gene            2072..2554
                     /gene="moaC"
                     /locus_tag="VM692_17035"
     CDS             2072..2554
                     /gene="moaC"
                     /locus_tag="VM692_17035"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HUQ53936.1"
                     /translation="MLSHVDAKNRPTMVDVGDKAVTTRTATARAVVVFPADVVVRSGE
                     ALRTKKGPVFDTAIIAGVMAAKRTHELIPFCHPIPLEDCKIVIDWGEGNEVVVECSVR
                     ATHKTGVEMEALTGASVAALTIYDMCKALTHGIAIREVVLLGKSGGKRDYTAAGQRSA
                     "
     gene            2551..2796
                     /locus_tag="VM692_17040"
     CDS             2551..2796
                     /locus_tag="VM692_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HUQ53937.1"
                     /translation="MTIEVTARYFAAFRERAGRDTERIETQARTAADLYGELAKRHGF
                     ADAMTRCKVAINDELADWNVALTAGDEVLFFPPVAGG"
     gene            2804..3244
                     /locus_tag="VM692_17045"
     CDS             2804..3244
                     /locus_tag="VM692_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007968990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HUQ53938.1"
                     /translation="MSSNAAFKIVTAVIDPVARKRELERTTAGACVTFEGWVRDHNDG
                     EAVQALEYEAHQAIAEKEGARIVAEALARFAVRAVHCEHRVGKLRIGDCAVWVGVSAD
                     HRGAAFDACRYVIDETKARVPIWKKEHYASGATGWVNCATRTAS"
BASE COUNT          604 a         1096 c         1165 g          616 t
ORIGIN      
        1 gaacgcgtcg aacgtgcccg tcagattacg gtagtcgtcc tcgacgaatt ccacttggcc
       61 gtcgagccct tgttgtttcg cccacgcgcg cgagtgtgcg atctgttcct tcgagatgtt
      121 gaagctgcgc actcgcacgc cgtaattttt ggccatgaat agcgcgaaac cgccccaacc
      181 gccgccggct tcgacgacgc tctcgccggc cttgagctgc agcttgcggc acacgtggtg
      241 catcttcgct tgctgcgctt gctcgatcgt catcgcaggg tcggcgaagt aggcgcacgt
      301 gtactgcagg gcctcgcggt cgagccacag cttgtagaag tcgttgccta ggtcgtagtg
      361 gtgatgaata ttgcgcttcg agtcgtgcag gtccggcgca agcttgcggc cgcgagcgag
      421 aacgtggccg atggcggtgt tctctgccgt gtagcgatag cactcgagca gcagagtcag
      481 cagatcgccg cgtacttgga ggcggcccga gctgtagaga tcgccgaaat gtaaatcggg
      541 gttgatgacg agctgccaga gagccttgct gtctagcacg cgcatgcgca cagtgccttc
      601 gcgcggtgcg acgtcgggct cgtcccataa gctgatgcga acgggcgaag ctccgagctt
      661 gctcgtgatt tgcttgacga gccaacggtc gagaccgctc ggctcgtggc gcgatgcctc
      721 cgcggcctcc aacccataat cggagtgggg aacgcccaac ggctccaaac ggctacgttc
      781 catggtgata ctccgcctgc aactccaaca ctgcccgggc tgtccaagcg tctcttgccg
      841 ctcggtctga cgccgagttc cgccccgcgg tgtgaggcgc tggcagcgtt tagcccgatg
      901 ccgaacctgc gggtctggcc cgcggttcgg tcccttctga gcgcgtaggt tactatagcg
      961 gcacgaaatt cggcagccgt gtctaacgcg gcagtgctgc gcgacgcacc gatatgacgc
     1021 acaccgtaac ccagtggcag acgcaagccg ccgtcaaaga ccggctgagc cgccctttgc
     1081 gcgacctgcg catctcggtc attgatcgct gcaactaccg ctgcccgtac tgcatgccgg
     1141 cggagctgtt cggcgagaac cacacgttcc tgccgcgcgc tcattggctg acgcccggcg
     1201 aaatcaagcg cgtcgcgagc gtgttcttgc agctcggcgt caagaaattc cgtctgacgg
     1261 gcggcgagcc gctgctgcgc cgcgacatcg tcgagatcgt cgccggccta gcggagctgc
     1321 atggcgccga ggatttggcg atcacgacca acggctcgcg gttgggcgag catgccgcag
     1381 cgctgaagca agccgggttg aagcggataa ccgtgagcct cgacagcctc gacgccgaag
     1441 tctttaaaga gatgaacggc ggtcgcggcg agctcgaggt cgtgttgcgc ggtatcgaag
     1501 ccgcgcgcgc cgagggtttc ccgatcaagc tcaataccgt cgtcgagcgc ggcaagaacg
     1561 atcacacgct gctcgagctc gtcgaccgct tccgcggcac cggcgtcatc gtgcggttta
     1621 tcgagtacat ggacgtcggc accgtcaacg gctggcagcc gcgccaagtc gtgccgtcga
     1681 aggagctgct ggcgcggttg cgcgagcgtt ggaccttgtc gccgctcgac agcaattacc
     1741 gcggcgaagt cgcgaagcgc tattcgttcg acgacgggca aggtgagatc ggcttcatct
     1801 cgtccgtcag cgagccgttc tgcggcgact gccatcgcgc gcgcttgtcg gcggacggca
     1861 ccgtctatac gtgcttgttc gcagcgagcg gcacggagtt gcggacgccg ctgcgctccg
     1921 gtgccagcga cgccgacctt gtcggcctgc tgcaaaacgt ctggcgcaac cgcacggatc
     1981 gctacagcga aatccgcggc cggttgctcg agcacaagcc cgagggcgtc gagaagcccg
     2041 agcgcgtcga gatgtaccgg atgggcggct gatgctgtcg cacgtcgatg cgaagaaccg
     2101 cccgacgatg gtcgacgtcg gcgacaaagc cgtcacgacg cggaccgcga ccgcgcgcgc
     2161 cgtcgtcgtt tttccggccg acgtcgtcgt gcgttccggt gaagcgctgc gcacgaagaa
     2221 agggcccgtg ttcgacaccg cgatcatcgc gggcgtcatg gccgcgaagc gcacgcacga
     2281 gctgattccg ttctgtcatc cgattcctct cgaagactgc aagatcgtta tcgattgggg
     2341 cgagggcaac gaagtcgtcg tcgagtgctc ggtgcgagca acgcacaaga cgggcgtcga
     2401 gatggaagcg ctgacgggcg cgagcgtcgc cgccttgacg atctacgaca tgtgcaaagc
     2461 gttgacgcac ggtattgcca ttcgcgaagt cgtcctgctc gggaaatccg gcggcaagcg
     2521 cgattacacg gccgcgggtc agaggtcggc gtgacgatcg aggtcacggc gcgctacttc
     2581 gcggcgtttc gcgagcgcgc cgggcgcgac accgagcgca tcgagacgca ggcgcgcacg
     2641 gcggcggatc tgtacggcga gctcgcgaag cgtcacggct tcgccgatgc aatgacgcgc
     2701 tgcaaggtcg cgatcaatga cgagctcgcc gattggaacg tagctctgac ggcaggcgac
     2761 gaggttttgt tcttcccgcc ggtcgccggc ggttagccgc ggcatgtcgt cgaacgcggc
     2821 gttcaagatc gtgaccgcgg tcatcgaccc ggtcgcacgc aaacgcgagc tcgaacgcac
     2881 cacggccggc gcctgtgtga cgttcgaggg ctgggtccgc gaccacaacg acggcgaagc
     2941 cgtgcaagcg ctcgagtacg aggcgcatca agcgatcgcc gagaaagaag gcgcgcgcat
     3001 cgtcgccgag gcgctcgcgc ggttcgccgt gagggccgtg cattgcgagc atcgcgtcgg
     3061 caagttgcgc atcggcgatt gcgccgtttg ggtcggcgtg agcgccgatc accgcggcgc
     3121 cgccttcgac gcctgccgct atgtcatcga cgagacgaaa gcccgcgtgc cgatctggaa
     3181 gaaagagcac tacgcgagtg gcgcgacggg ctgggtcaac tgcgcgacta gaaccgccag
     3241 ctagccttgc gcggcggcca gtccggcgcc gcccgcaaat tcgccttgtg gtgcgccgct
     3301 gttcgcatta tgtttagcgc tggtcgcggg ctgtccaggg aggacgagca atgaagacat
     3361 ggagtcgtat tgcgctggcc gcgagcttgg ctctcgcagc caatgtcgcc ggcgccattc
     3421 ccctacaaat caacgtgcag accgtctcga tcggcggctc gcagggctca tggtcgctgc
     3481 t
//
LOCUS       DATLEE010000776         2635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6563389, whole genome shotgun sequence.
ACCESSION   DATLEE010000776 DATLEE010000000
VERSION     DATLEE010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6563389"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VM692_17050"
     CDS             <1..1030
                     /locus_tag="VM692_17050"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUQ53939.1"
                     /translation="PYEKRWHVGMWMVPSYDPELRRLYVGTSVTAPAPKFMLGGNDEQ
                     YLYHNSTLALDIDTGKIVWHFQHVVDHWDLDHPFERLLVETETAPDASAVKWINPRVR
                     PGERRKVITGIPGKTGIAYTLDRETGQFLWARETVEQNVISDINVESGAATVNPDKIF
                     TAAGQEKLICPSTSGGKNYPSGTYSPLTGLMYFPLQNTCMMATATQAEINTDVTYALR
                     NRVIMTPGAENVGTIEAINVSTGQTAWKIEQRAGALSLMSTAGGLLFGGDTNGRFRAY
                     DQRNGEVLWEINLGAPVNGYPAAFAVNGKQYVAVSTGGSGLALGLARLAPEQKPGNGN
                     QLFVFALP"
     gene            1061..2395
                     /locus_tag="VM692_17055"
     CDS             1061..2395
                     /locus_tag="VM692_17055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUQ53940.1"
                     /translation="MQTATPPSEATRNGILTTLGATIGLAFGPSVIANLTVTGYITPI
                     EREFGWSRVDVSFAFTIVAYMIVLMSPLQGFLVDRFGPRRVVLTSIPLFALSLAAIYF
                     TPPNLYVYYLLWAIVPVAGIGLWPLGYLQAVTPWFDRKLGLALGSANAGIGVGSTLLP
                     MFVIAPIIAASATDDGLLMQFVTVGGLFPFAGWRSAVLTLSLLIVIVSWPVVWYCLRE
                     PSAAEVAARKQTGAAKSFGLSFQEARREPTFWILNLGFFLLGITATSLVTQQAPLLQD
                     AGWTPAETARLQTTFGFGLLFARVAVGYVIDHIFAPRVLTAVSLGGVVACILYAVYPD
                     VGYVSALLIGFLLGAEFDVLAFLIKRYYGNVAYGRLYGVIFGVFYLGSGIGIWGLPKI
                     RELSGNNYDNGLYVAAVILFASAILMSFMPKYRYSVGHTAEPIAGNHATQTA"
     gene            2413..>2635
                     /locus_tag="VM692_17060"
     CDS             2413..>2635
                     /locus_tag="VM692_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrrolo-quinoline quinone"
                     /protein_id="HUQ53941.1"
                     /translation="MRLPATTTMTAVCAAVLGALAWAATAQQQQRTLRPVTDAMLQNP
                     DAADWLSWRRTLNHWGYSPLNEINKRNVAQ"
BASE COUNT          443 a          882 c          825 g          485 t
ORIGIN      
        1 tccgtacgag aagcgttggc acgtcggcat gtggatggtg ccgagctacg atcccgagct
       61 gcgccgcctg tatgtcggca cttccgtgac ggcgccggcg ccgaagttca tgctcggcgg
      121 gaacgacgag cagtacctct atcacaactc gacgctcgcg ctcgacatcg acaccggcaa
      181 gatcgtctgg cacttccagc acgtcgtcga tcactgggat ctcgatcatc cgttcgagcg
      241 cctgctcgtt gaaacggaga ccgcgcccga tgcgtctgcc gtgaaatgga tcaatccgcg
      301 cgtgcgtccc ggcgagcggc gcaaagtcat cacgggcatc ccgggcaaga ccggcatcgc
      361 ctacacgctc gatcgtgaga cgggtcagtt tctctgggcg cgcgagaccg tcgagcagaa
      421 cgtgatctcg gacatcaacg tcgagtccgg cgccgcgacc gtcaatcccg acaagatatt
      481 cacggcggcc ggccaagaga agctgatttg tccgagcacg tcgggcggca agaattatcc
      541 gagcggcacg tacagtccgc tgacggggct catgtacttc ccgctgcaga acacctgcat
      601 gatggcgacc gcgacgcagg ccgagatcaa caccgacgtc acgtacgcgc tgcgcaatcg
      661 cgtcatcatg acgccaggcg cggagaacgt cggcacgatc gaggcgatca acgtgtcgac
      721 ggggcaaacg gcgtggaaaa tcgagcagcg cgccggcgcc ctgagcttga tgtcgacggc
      781 cggcggcttg ctgttcggcg gcgacacgaa cggccgcttc cgcgcgtacg atcagcgcaa
      841 cggcgaagtg ctctgggaga tcaacttggg cgcgcccgtc aacggctacc cggcggcgtt
      901 cgccgtcaac ggcaaacaat acgtcgccgt cagcacgggc ggctctggcc tggcgctcgg
      961 tctcgctcgg ctcgcgccgg agcaaaaacc gggcaacggc aaccagctgt tcgtcttcgc
     1021 actgccataa cgaatatagg tcgcgctccg gggggaacgc atgcagacag ccactccgcc
     1081 gtccgaagcc acgcgtaacg gcatcttgac gacgctcggc gccacgatcg gtctggcgtt
     1141 cggcccgagc gtcatcgcga acctcacggt caccggctac atcacgccga tcgagcgcga
     1201 gttcggctgg tcgcgcgtcg acgtctcgtt cgcgttcacg atcgtcgctt acatgatcgt
     1261 gctgatgtcg ccgctgcagg gctttctcgt cgaccgtttc ggcccgaggc gcgtcgtgct
     1321 gacgtcgatt ccgctgttcg cgttgagctt ggcagcgatc tatttcacgc cgccgaacct
     1381 ctacgtctac tacttgctct gggcgatcgt gcctgtcgca ggcatcggcc tctggccgct
     1441 cggctacctg caagccgtga cgccatggtt cgaccgcaag ctcgggctcg cgctcggcag
     1501 cgcgaacgcg ggtatcggcg tgggcagcac gctgctaccg atgttcgtca tcgccccgat
     1561 catcgcggcc agcgcgacgg acgacggctt gctgatgcaa ttcgtgacgg tcggcggctt
     1621 gtttccgttc gccggttggc gctcggccgt gcttaccttg agcttgttga tcgtcatcgt
     1681 gagctggccc gtcgtttggt actgtctgcg cgagccgtcc gcggccgaag tcgcggcgcg
     1741 caagcaaacc ggcgcggcga agtcgttcgg cttgtcgttt caagaagcac ggcgtgagcc
     1801 gacgttctgg atcttgaacc tcgggttctt cctgctcggc atcacggcca cgagcctcgt
     1861 cacgcagcaa gcgcccctgc tgcaagacgc cggctggacg cccgccgaga cggcgcgcct
     1921 gcaaacgacg ttcggcttcg gcctgctgtt cgcgcgcgtc gcggtcggtt acgtcatcga
     1981 ccacatcttc gcgccgcgcg tgctgacggc ggtgtcgctc ggcggcgtcg tcgcctgcat
     2041 cctctacgcc gtctaccccg acgtcggcta cgttagcgcg ctgctgatcg ggttcttgct
     2101 cggcgccgag ttcgacgtgc tcgcgttcct gatcaaacgc tactacggca acgtggccta
     2161 cgggcgactc tacggcgtca tcttcggcgt gttctatctc ggctcgggta tcggcatctg
     2221 gggactgccg aagatccgcg agctctcggg caacaactac gacaacgggc tctacgtggc
     2281 cgcggtcatc ttgttcgctt cggcgatcct gatgtcgttc atgccgaagt accgctactc
     2341 cgtcgggcat accgccgagc cgatcgccgg caaccacgcg acccaaactg cttgaactga
     2401 gccggagctt taatgcgatt gcctgcaacc acgacgatga cggccgtgtg cgccgccgtg
     2461 ctcggcgctc tcgcctgggc cgccactgca caacaacaac aacgcacgct gcgtcctgtg
     2521 acggacgcga tgctgcagaa tccggacgcc gccgactggc tgtcgtggcg ccgcacgctg
     2581 aaccactggg gctatagccc gctcaacgag atcaacaagc gcaacgtcgc gcagc
//
LOCUS       DATLEE010000777         4116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8123237, whole genome shotgun sequence.
ACCESSION   DATLEE010000777 DATLEE010000000
VERSION     DATLEE010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8123237"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VM692_17065"
     CDS             complement(<1..963)
                     /locus_tag="VM692_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HUQ53942.1"
                     /translation="MTSRTRATRSAAHFGAGVLAVLALAAPSAGQAPHYLATRKIDHV
                     DDYHGTKVPDPYRWLEDETSAETAAWVEAQNAVTFPYLERIPYRGALLERVTELNDYE
                     RFSAPAQKGPYFFFSKNSGLQNQSVLYIQAGANGAPEVLLDPNSWSADGTVQLAAFEP
                     SRDAKYAVYGISQSGSDWQQYKVMELATKRTLSDTVDWVKVSNVAWHGDGFYYSRYPE
                     PPEGHEKASINENHLVYFHKLGTPQSADTQVYEDAANPQRFHLVYTTEDERFAILEVS
                     ERGKGKDGNALFVRDLAKPDDAFRPLVPEIGNDTFTVVDNVGGEI"
     gene            complement(1004..2833)
                     /locus_tag="VM692_17070"
     CDS             complement(1004..2833)
                     /locus_tag="VM692_17070"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HUQ53943.1"
                     /translation="MSLLSRVAERIYWTARYLERAETTSRLARVYGNLLLDLPQEAGL
                     SWDVLVQITGGEDRYRELHPSPGVDTAERFFVADRENPSSVRSSLQLARESVRTTRDI
                     VPSEVWRSINELTLWVTQELDAETSHRRRYGIHSRVVERCQQITSLLADTMSHDAAYQ
                     FFRLGRGIERADMTTRIIDVAATMLLGREGLARFDNSLWMAVLQSLSAYQMYRQKVRR
                     RIYGPDVIAYLLKDPQFPRAVAFSLRETGAALGMLPRSGEPIKKLGDLRRMLALRNLS
                     ELSIAELHQWMDDAQLRLNELHERVAATWFRPSAPSQEPASRVYDIRHKTTFSYQHVV
                     SSSRHVLHLLPRRHPSQTCLLAETIVDPRPAAESVGEDDFGNPVQRLAVEQPHKVLVV
                     DAHARIEVRGAAAPALEQSPPWESIRDRLTGDPSLAERDYLAASPHVAANDDVRAYAL
                     PSFPPGRPILAAVMDLTQRIYRDFEYRGGVSDVWTPVSDVMAMRAGVCQDFAHLEIAC
                     LRSVGLAARYVSGYLLTRPLRGEAKLVGADASHAWVSVFAGDAGWVDFDPTNNVIPKI
                     EHITFAWGRDYGDVSPISGTIVGGGAHQVSVAVDVNPTPAGLA"
     gene            complement(2830..>4116)
                     /locus_tag="VM692_17075"
     CDS             complement(2830..>4116)
                     /locus_tag="VM692_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HUQ53944.1"
                     /translation="VEMGITFTVYTEGQNIDRAWPFDVIPRTIDAGEWARIESGLIQR
                     LRALNHFIDDLYHDRRAIKDGVVPDDIIATAANFLPACIGSKPPYGVWANICGTDLVR
                     DRDGTVYVLEDNLRVPSGVSYMLENRMVTKQVFPEMFEHASILPVDDYASQLLDMLSS
                     LSPRKVEFPRVVVLTPGIYNSAYFEHSYLAQRMGVDLVEGQDLVVADDECVYVRTIRG
                     LERVDVIYRRVNDTFIDPECFDERSVLGVPGLMRAWRAGNVALANAPGAGVADDKVVY
                     AYVPQIIEYYLGEKAILPNVPTLLLRDADSRKRVLDDLSQWVVKPANESGGYGMLIGP
                     RATDEEKQAMAAAVEANPRNYIAQPVLALSTAPTLVHGEIEPRHLDLRPFILQGRNVW
                     VTPGGLTRVALRRGSLVVNSSQGGGSKDTWIVAVRR"
BASE COUNT          654 a         1363 c         1397 g          702 t
ORIGIN      
        1 gatctcgccg ccgacattgt cgacgaccgt gaacgtgtcg ttgccgatct ccggcacgag
       61 cggccggaac gcgtcgtcgg gcttggcgag gtcgcgcacg aacagcgcgt tgccgtcctt
      121 acccttgccg cgctccgaga cctcgaggat cgcgaagcgc tcgtcctccg tcgtgtagac
      181 gaggtggaag cgctgcgggt tcgcggcgtc ctcgtaaacc tgggtgtccg ccgactgcgg
      241 cgtgccgagc ttgtggaaat aaacgagatg gttctcgttg atcgacgcct tctcgtggcc
      301 ctcgggcggc tccggatagc ggctgtagta aaacccgtcg ccgtgccacg cgacgttcga
      361 caccttgacc cagtcgaccg tgtcgctgag tgtccgcttc gtcgcgagct ccatgacttt
      421 gtactgctgc cagtccgagc cgctctgcga gatgccgtag acggcgtact tcgcgtcgcg
      481 cgacggttcg aaggcggcga gctgcacggt gccgtcggcc gaccacgagt tggggtcgag
      541 caacacctcg ggtgcgccgt tcgcaccggc ttggatgtac agcacgctct gattttgtag
      601 gcccgagttc ttgctgaaga agaagtacgg gcccttctgc gcgggcgccg agaagcgctc
      661 gtagtcgttg agctccgtca cgcgctcgag cagcgcgccg cgatacggaa tgcgctcgag
      721 ataggggaac gtgactgcgt tctgcgcttc gacccacgcc gccgtctcgg ccgaggtctc
      781 gtcctcgagc cagcggtagg gatcgggcac tttggtgccg tgatagtcgt ccacgtgatc
      841 gatcttcctc gtcgcaagat agtgaggcgc ctggcccgcg ctcggcgccg ccagcgcgag
      901 caccgcgagc actccggccc cgaaatgcgc ggcacttcgt gtcgcgcgcg ttcgtgacgt
      961 catcgtccct ccccattggg ccggcacgcc ggcctcgaga caattatgcc aggccggcgg
     1021 gcgtgggatt cacatcgacg gcgacgctga cctggtgcgc gccgccgccg acgatcgtgc
     1081 cgctgatcgg gctgacgtcg ccgtagtcgc ggccccaggc aaacgtgatg tgctcgatct
     1141 tcgggatcac gttgttcgtc gggtcgaaat cgacccagcc cgcgtcgccg gcgaacacgg
     1201 agacccatgc gtgcgacgcg tcggcgccga cgagctttgc ctcgcccctc agcggcctcg
     1261 tcaacagata accgctgacg tagcgcgccg cgagcccgac gctgcgcaag cacgcgatct
     1321 cgaggtgcgc gaagtcctgg cagacgccgg cacgcatcgc catgacgtcg ctgacgggcg
     1381 tccagacatc cgacactccg cctcgatact cgaagtctcg atagatgcgc tgcgtgaggt
     1441 ccatgaccgc ggcgaggatc ggccggcccg gcgggaacga cggcagcgcg taggcacgaa
     1501 catcgtcgtt ggcggcgacg tgcggcgacg cggccagata gtcgcgctcg gcgagactag
     1561 ggtcgccggt gagccggtcg cgaatgcttt cccacggcgg gctctgctcg agcgccggcg
     1621 ccgccgcgcc gcgcacctcg atgcgtgcgt gagcgtccac gacgaggacc ttgtgcggct
     1681 gctcgacggc aaggcgctgc acgggattac cgaagtcgtc ctcgccgacg gactcggcgg
     1741 ccggtcgagg gtcgacgatc gtctcggcga gcaggcacgt ttgcgacgga tgccgtcgcg
     1801 gcagcaagtg caacacgtgc cgcgagctcg acacgacgtg ctgatagctg aacgtcgtct
     1861 tgtggcgaat gtcgtaaacg cggctcgccg gttcctggct cggtgcgctc ggtctgaacc
     1921 acgtcgctgc gacgcgctcg tgcagctcgt tcaaccggag ttgcgcgtcg tccatccatt
     1981 gatgcagctc ggcgatcgag agctcgctta aattgcgcag cgcgagcatg cggcgcaggt
     2041 cgccgagttt cttgatcggc tcgccgctgc gcggcagcat cccgagcgcc gcgccggtct
     2101 cgcgcaggct gaacgcgacc gcgcgcggaa actgcgggtc cttgagcaga tacgcgatga
     2161 cgtcgggccc gtagatgcgc cggcgcacct tctggcgata catctgatag gccgacagcg
     2221 actgcagcac ggccatccac aacgaattgt cgaagcgcgc gagcccttca cggccgagca
     2281 gcatcgtggc tgcgacgtcg atgatgcgcg tcgtcatgtc ggcgcgctcg atgccgcggc
     2341 ccaagcgaaa gaactgatac gcggcgtcgt ggctcatcgt gtccgcaagc aagctcgtga
     2401 tctgctgaca gcgctcgacg acacgcgagt ggatgccgta gcgtcgccgg tgcgacgtct
     2461 cggcgtcgag ctcttgcgtg acccagagcg tcagctcgtt gatcgaccgc cacacttctg
     2521 acggcacgat gtcgcgcgtc gtgcgcacgc tctcgcgagc gagctgtagc gacgagcgca
     2581 cggagctcgg gttctcgcga tcggcgacga agaagcgctc ggccgtgtcg acgccggggc
     2641 tcggatgcag ctcgcggtag cggtcttcgc cgcccgtgat ctgcacgagc acgtcccaag
     2701 acagcccggc ctcctgcggc aggtcgagca gcagattgcc gtagacgcgc gcgagccgcg
     2761 acgtcgtctc ggcgcgctcg agatagcgcg ccgtccagta gatgcgctcg gcgacgcgcg
     2821 acagcaggct catcgcctga ccgccacgat ccaggtgtcc ttgctgccgc cgccctgcga
     2881 cgagttcacg acgagcgagc cgcgccgcag cgcgacgcgc gtcaagccgc ccggcgtgac
     2941 ccagacgttg cggccttgca ggatgaacgg ccgcagatcg agatgccgcg gctcgatctc
     3001 gccgtgcacg agcgtcggcg ccgtcgacag cgccagcacg ggctgggcga tgtagttgcg
     3061 cgggttagcc tcgacggcgg ccgccatcgc ctgcttctct tcgtcggtcg cgcgcgggcc
     3121 gatcagcatg ccgtagccgc ccgactcgtt cgcgggcttg acgacccact ggctcaagtc
     3181 gtcgagcaca cgcttgcggc tgtcggcatc gcgcagcagc agcgtcggca cgttcggcag
     3241 gatcgccttt tcgcccaagt agtactcgat gatctgcggc acgtacgcgt agacgacctt
     3301 gtcgtcggcg acgccggcgc cgggcgcgtt cgcgagcgcg acgttgcccg cgcgccaggc
     3361 gcgcatgagg cccggcacgc cgagcacgga gcgctcgtcg aagcactcgg ggtcgatgaa
     3421 cgtgtcgttg acgcgccgat aaatgacgtc gacgcgctcg aggccgcgga tcgtgcgcac
     3481 gtagacgcac tcgtcgtcgg cgacgacgag gtcctgccct tcgacgagat cgacgcccat
     3541 gcgctgcgcg agatacgagt gctcgaagta cgcggagtta tagatgcccg gcgtcaacac
     3601 gacgacgcgc gggaattcca ctttgcgcgg gctcaggctg ctcaacatgt cgagcagctg
     3661 cgaggcgtag tcgtcgacgg gcaagatcga cgcgtgctcg aacatctcgg ggaacacttg
     3721 cttcgtcacc atgcggttct cgagcatgta cgagacgcca gacggcacgc gcaggttgtc
     3781 ctcgagcacg tacacggtgc cgtcgcggtc gcgcaccaag tcggtgccgc agatgttggc
     3841 ccagacgccg tacggcggct tgctgccgat gcatgcgggt aaaaaattgg ccgccgtcgc
     3901 gatgatgtcg tcgggcacga cgccgtcttt gatcgcgcgt cggtcgtgat acagatcgtc
     3961 gatgaagtga ttcagcgccc tgagccgctg gatgaggccg ctctcgatcc gggcccattc
     4021 accggcgtcg atcgtgcgcg ggatgacgtc gaacggccac gcgcggtcga tgttctggcc
     4081 ttccgtgtag accgtgaacg tgatgcccat ctcgac
//
LOCUS       DATLEE010000778         5568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8123377, whole genome shotgun sequence.
ACCESSION   DATLEE010000778 DATLEE010000000
VERSION     DATLEE010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8123377"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /gene="ftsA"
                     /locus_tag="VM692_17080"
     CDS             complement(<1..492)
                     /gene="ftsA"
                     /locus_tag="VM692_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HUQ53945.1"
                     /translation="MGRRDDRKLVVGLDIGTSKVVAIVGELSADDRLEVIGLGSNPSR
                     GLKRGVVVDIESTVQSIQRAIEEAELMAGCEINSVLTGIAGSHVRSLNSHGIVAIKDK
                     EVSAGDVERVIDAARAVAIPADQRVIHVLPQEFIIDSQEGIREPIGMSGVRLEAKVHL
                     VTGA"
     gene            complement(525..1337)
                     /locus_tag="VM692_17085"
     CDS             complement(525..1337)
                     /locus_tag="VM692_17085"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HUQ53946.1"
                     /translation="MFGRKANRRRAESRVARLKVPALGAAWVRRLRTALMVPIAAVAC
                     YGIFKGVQFVLDQPIRNLEVEGTFQRVTPIQVEAAVADGLKAGFLTVNLAALRERVQA
                     LDWVDRASVGRRWPDTLLVRVTEHQAAARWGESGLLNVRGELFTEHSQHAFAELPSLA
                     GPPGSERDVARRYLAVRGKLVEADLSLETLEMDERGSWRLVLRGGQEIRLGRRDIDER
                     LHRFFRVVAPVLAAELKSVEYVDLRYTNGFAVGWREGPPAGLAAVAAVPSGG"
     gene            complement(1337..2254)
                     /gene="murB"
                     /locus_tag="VM692_17090"
     CDS             complement(1337..2254)
                     /gene="murB"
                     /locus_tag="VM692_17090"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HUQ53947.1"
                     /translation="MNAAPNERAAIEVLRNEPLRKHTSWRVGGPADLFYTPTSTAELQ
                     RILADLPPATPVHWLGLGSNLLVRDGGIRGAVIATGSLPRELERLDSHKVRASAGLAC
                     MLLAKRCVRWQLGPAAFFAGIPGTVGGALAMNAGAFGGETWAHVAAVTTIDRRGELHE
                     RARAEFEIDYRSVRGPANEWFLAATFRFDHDASSSMEAIKELLARRNAAQPLGIPSCG
                     SVFRNPPGDFAGRLIEQAGLKGKQLGGARVSDKHANFILNAKNATAADIEALIHEVRA
                     AVERTSGVQLELEVRVIGEPGVATPKGEP"
     gene            complement(2251..3675)
                     /gene="murC"
                     /locus_tag="VM692_17095"
     CDS             complement(2251..3675)
                     /gene="murC"
                     /locus_tag="VM692_17095"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HUQ53948.1"
                     /translation="MTTRMGKTRSIHFIGIGGAGMAGIAEVLLNLGYTVSGTDVKLSS
                     VTERLRGLGARVAEGHAAAHLGDTDVVVVSSAVAADNPEIVAAHANRIPVVRRAEMLA
                     ELMRFRYGIAIAGTHGKTTTTSLIASVLAEAGVDPTFVIGGRLASANANAKLGLGQYL
                     VAEADESDASFLHLQPMLAVVTNIDADHLGSYENDLGRLKQGFLDFLHNLPFYGLAVM
                     CSDDANTRELIPSLGRRVVSYGFGERADVRASAVERFGLKTRFKVETKERREPLTVEL
                     NLPGMHNVRNSLAAIAVAAELQIADAPLLKALAEFSGVDRRLQVQGTVATAAGRVTFV
                     DDYGHHPTELAATIAAARDAWPGRRLVVCFQPHRYTRTLALIDDFAQVLSTVDALILT
                     NVYSAGETPLAGADGKSLARAVRVRGAVEPIFIEDLAELPATLSQILKADDVVLTLGA
                     GSIGAAAQTLPRALAVLAPVGVQK"
     gene            complement(3672..4763)
                     /gene="murG"
                     /locus_tag="VM692_17100"
     CDS             complement(3672..4763)
                     /gene="murG"
                     /locus_tag="VM692_17100"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HUQ53949.1"
                     /translation="MSSGPIMIMAGGTGGHVFPGLAVAQALRARDRAVVWLGTQRGIE
                     ARVVPANGIEIEWITIAGVRGRGLVAYVTAPFRMAAAVAQALAALRKRRPSAVLGMGG
                     FAAGPGGLAAWLTRRPLLIHEQNSVAGTTNRMLAPFAARVFEAFPDSFPRGARAELVG
                     NPVRASMVSSDVPRERLDARRRERRRLFVIGGSQGARVFNETLPLALAELEPDRRPEI
                     WHQAGKLGVDEARAAYAAAGVEARVDAFVDDVSSAFRWADLVVARAGASTLAELAIVG
                     VAAILVPLPTAIDDHQTTNARHYTAGGAGVAVAQRDLQPAAFAQLLASCLGDMPRLIA
                     MAEAARSEARLDAADRVARACIELAEVRA"
     gene            complement(4760..>5568)
                     /locus_tag="VM692_17105"
     CDS             complement(4760..>5568)
                     /locus_tag="VM692_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HUQ53950.1"
                     /translation="ASEPARLCLLLYLASYAVRRSTQLGASLNGLVRPLFVVAAAAVL
                     LLAEPDFGATVVLGTTALVVLFVGGARLRDLLLAVLVGGTLLTVLAFSADYRVVRLMS
                     FRDPWADPFATGFQLTQALMAIGRGEWGGVGLGESVQKLFYLPEAHTDFVFAVLVEEL
                     GFAGATLVIGLFGIVIYRSITLGREALANGMHFQGLVATGIGVMLGFEAFVNMGVNAG
                     LLPTKGLPLPLISYGRASTVITLVALGFLLRIHHELYGGQKRLVQRGYVR"
BASE COUNT          913 a         1988 c         1845 g          822 t
ORIGIN      
        1 ggcgcccgtc acgagatgca ccttggcttc gagccgcacg cccgacatgc cgatcggctc
       61 gcgaatgcct tcctgcgagt cgatgatgaa ttcctgcggc agcacgtgga tgacgcgctg
      121 atcggccgga atcgcgacgg cgcgcgccgc gtcgatgaca cgctcgacgt cgccggcgct
      181 gacttccttg tccttgatcg cgacgatgcc gtgcgagttc aagctgcgca cgtggctacc
      241 ggcgatgccc gtcagcacgc tgttgatctc gcagccggcc atcagctcgg cctcctcgat
      301 cgcgcgctgg atcgattgca ccgtcgactc gatgtcgacg acgacgccgc gcttgagccc
      361 gcgcgacggg ttcgacccca agccgatgac ctcgagccga tcgtcggccg atagctcgcc
      421 gacgatcgcc acgactttcg acgtgccgat gtcgagaccg accacgagct tgcggtcgtc
      481 gcgtctaccc actactcgaa tccccgttgc actgcactca tcctttaacc gccactcggt
      541 accgccgcga cggcggcgag accggccgga ggtccctcgc gccaaccgac ggcgaaaccg
      601 ttcgtgtagc gcagatccac gtactcgacg ctcttgagct ccgcggccag cacgggtgcc
      661 acgacgcgga agaatcggtg gagccgctcg tcgatgtcgc ggcggccgag cctgatctct
      721 tggccgcctc gcagcacgag gcgccaggag ccgcgctcgt ccatttccaa ggtttccagc
      781 gacaagtccg cctcgacgag cttgccgcgc acggcgagat agcggcgcgc gacgtcgcgc
      841 tcgctgcccg gcgggcccgc gaggctcggc aattcggcga acgcgtgctg cgaatgctcg
      901 gtgaacagct cgccgcgcac gttcaatagg ccgctctcgc cccagcgcgc ggcggcttga
      961 tgctccgtga cccgcacgag caacgtatcg ggccaacgtc ggccgacgct cgctcgatcg
     1021 acccagtcga gcgcctggac gcgctcccgc agcgccgcga gattgaccgt gagaaatccg
     1081 gccttgagcc catcggcgac ggccgcctcg acttgaatcg gcgtcacgcg ctggaacgtt
     1141 ccctcgacct cgagattgcg gatcggctga tcgaggacga actgcacgcc tttgaagatg
     1201 ccgtagcagg ccactgccgc gatcggcacc atgagcgccg tgcgcagccg ccgcacccac
     1261 gccgcgccga gcgccggcac cttcaggcgc gcgacgcggc tttcggctct gcggcgattg
     1321 gctttacggc cgaacatcat ggctctccct taggcgttgc aacgccgggc tcgccgatga
     1381 ctcgcacctc gagctcgagc tgcacgccgc tcgtgcgctc gaccgccgca cgcacctcgt
     1441 ggatcagcgc ctcgatatcg gccgccgttg cgttctttgc gttcaggatg aagttcgcgt
     1501 gcttgtccga caccctcgca ccgccgagct gcttgccctt gagcccggcc tgctcgatca
     1561 gacgacccgc gaaatcgccc ggcggattgc ggaacacgga gccgcagctt gggattccaa
     1621 ggggctgcgc agcgttgcgc cgcgcgagca actccttgat ggcctccatg gagctcgacg
     1681 cgtcgtggtc gaagcggaac gtcgcggcga ggaaccactc gttggccggg ccgcgcacgc
     1741 tgcgatagtc gatctcgaac tccgcgcggg cgcgctcgtg cagctcgccg cggcgatcga
     1801 tcgtcgtcac ggctgcgacg tgcgcccaag tctcgccgcc gaacgcgccg gcgttcatcg
     1861 cgagagcacc gccgaccgtg cccggtatgc cggcgaagaa cgccgccggg cccaactgcc
     1921 aacgcacgca gcgcttcgcg agcaacatgc acgcgagccc ggcgctcgcg cgcactttgt
     1981 gcgagtcgag gcgctctagc tcgcgcggca aactgcccgt cgcgatgacg gccccgcgaa
     2041 tgccgccgtc gcgcacgagc aaattcgagc cgaggccgag ccaatgaacg ggcgtcgccg
     2101 gcggcaaatc cgcgagaatg cgttgtagct cggccgtgct cgtcggcgtg taaaacagat
     2161 ccgcgggtcc gccgacgcgc cacgacgtgt gcttgcgcaa cggctcgttg cgcagcacct
     2221 cgatcgcggc tcgttcgttc ggggctgcgt tcacttttgc accccgaccg gcgcgagcac
     2281 ggcgagcgcg cgcggcaacg tctgcgcagc cgcgccgatg ctgccggcgc cgagcgtcag
     2341 cacgacgtcg tcggccttga gtatctggct caacgtcgcg ggcagctcgg ccaagtcctc
     2401 gatgaagatc ggctcgacgg caccgcgcac gcgcacggct cgcgccagcg acttgccatc
     2461 ggcgccggca agcggcgtct cgccggccga atagacgttc gtaagaatca acgcgtcgac
     2521 ggtcgacaac acctgcgcga agtcgtcgat cagcgccagc gttcgtgtgt agcggtgagg
     2581 ttggaagcag acgacgagcc ggcggcctgg ccacgcgtcg cgcgccgcgg cgatcgtcgc
     2641 ggccagctcg gtcggatggt gaccgtagtc gtcgacgaac gtcacgcggc ccgccgccgt
     2701 cgcgacggtg ccttgcacct gcaagcggcg atcgacgccg gagaactcgg cgagcgcttt
     2761 cagtagcggc gcatcggcga tctgcagctc cgccgccacg gcgatcgccg cgagcgagtt
     2821 ccgcacgttg tgcatgccgg gcaaattcag ctcgaccgtg agcggctcac gtcgctcttt
     2881 cgtctcgacc ttgaagcgcg tcttgagacc gaaccgctcg acggccgagg cgcggacgtc
     2941 ggctcgctcg ccgaacccgt agctgacgac gcgccgcccg agcgacggga tcagctcgcg
     3001 cgtgttcgcg tcgtcgctgc acatcacggc gagcccgtag aacggcaggt tgtgcaggaa
     3061 gtcgaggaag ccttgcttca aacggccgag gtcgttctcg tagctgccga gatgatcggc
     3121 gtcgatgttc gtgacgacgg ccagcatcgg ctgcagatgc aggaacgagg cgtcgctctc
     3181 gtcggcttcg gctacgaggt actggcccaa cccgagcttc gcgttggcgt tggcgcttgc
     3241 gagccggccg ccgatcacga acgtcggatc gacgcccgct tcggcgagca cgctcgcaat
     3301 caggctcgtc gtcgtggtct tgccgtgcgt gccggcgatt gcgatgccgt agcggaagcg
     3361 catgagctcg gccaacatct cggcgcggcg cacgacgggg atgcggttcg cgtgcgccgc
     3421 cacgatctcg ggattgtcgg cggccacggc gctcgagacg acgacgacgt ccgtgtcgcc
     3481 caagtgagcg gctgcgtgcc cttcggcgac gcgggcgccg agcccgcgca accgctccgt
     3541 cacgctgctc agcttcacgt ccgtgccgct gaccgtgtag cccaagttca gcagcacctc
     3601 ggcgatgccc gccatgccgg cgccgccaat gccgatgaaa tggatgctgc gagttttgcc
     3661 catgcgcgtc gtcatgcgcg cacctcggca agctctatgc aggcacgcgc gactcggtcg
     3721 gccgcatcca gccgcgcctc gctgcgcgcg gcctccgcca tcgcgatcaa gcgcggcatg
     3781 tcgccgaggc agctcgcgag caactgcgcg aacgcggccg gttgcagatc gcgctgcgcg
     3841 acggcgacgc cggccccgcc cgccgtgtaa tgacgcgcgt tcgtggtttg atggtcgtcg
     3901 atggccgtcg gcagcgggac caagatcgcc gcgacaccga cgatcgcaag ctcggctaac
     3961 gtgcttgcgc ctgcgcgcgc aacgacgagg tccgcccaac ggaacgcgct cgacacgtcg
     4021 tcgacgaacg cgtcgacgcg cgcctcgaca cccgcggccg cgtacgcggc acgcgcttcg
     4081 tcgacgccga gcttgccggc ttggtgccag atctccggcc gccggtccgg ctcgagctcg
     4141 gcgagagcca gcggcagcgt ctcattgaag acgcgcgcac cctggctgcc gccgatcacg
     4201 aacaagcgcc gccgttcacg gcggcgcgcg tcgagtcgct cgcgcggaac gtcgctcgaa
     4261 accatcgagg cacgcacggg gttgccgacg agctcggcgc gcgcgccgcg cgggaagctg
     4321 tcgggaaacg cctcgaagac tcgcgccgca aacggtgcca gcatgcgatt ggtcgtgccg
     4381 gccaccgagt tctgctcgtg gatcagcagc ggtctgcgcg tcaaccaagc cgcgagcccg
     4441 ccgggcccgg ccgcgaagcc ccccatgccg agcacggccg acggtctgcg tttgcgcagc
     4501 gcagcgagcg cttgtgcgac agccgcggcc atgcgaaacg gcgcggtcac gtacgccacg
     4561 agaccgcggc cgcgcacgcc cgcgatcgtg atccactcga tctcgatacc gttcgcgggc
     4621 acgacacgcg cttcgatgcc gcgctgcgtg ccgagccaga cgacggcgcg gtcgcgcgcg
     4681 cgcaaagcct gcgcgacggc gaggccgggg aacacgtgcc cgcccgttcc gcctgccatg
     4741 atcatgatcg ggcccgagct catcggacgt acccgcgctg cacgaggcgc ttctggccgc
     4801 cgtagagctc gtgatggatg cgcagcaaga agcccagcgc gacgagcgtg atgacggtgc
     4861 tcgcacggcc atagctgatc aacggcagcg gcagtccctt cgtcggcaac aaacccgcgt
     4921 tgacgcccat gttgacgaag gcctcgaagc ccagcatgac gccgataccc gtggcgacga
     4981 gcccttggaa gtgcatgccg ttggcgagcg cctcgcgacc cagcgtgatc gagcgataga
     5041 tcacgatgcc gaacaagccg atcacgagcg tggcgccagc gaagccgagc tcctcgacga
     5101 gcaccgcaaa cacgaagtcg gtgtgcgctt cgggcaaata gaaaagcttc tgcacgcttt
     5161 caccgaggcc gacgccgccc cactcgccgc ggccgatggc catcagcgct tgcgtcaact
     5221 ggaagcccgt cgcgaacgga tcggcccacg gatcgcggaa cgacatcagg cgcacgacgc
     5281 gatagtcggc cgagaatgcg agcaccgtca gcagcgtgcc gccgacgagc acggccagca
     5341 gcaaatcgcg cagccgcgct ccgccgacga acagtacgac gagagccgtc gtgccgagca
     5401 ccacggtcgc gccgaagtcg ggctcggcca gcagcagcac cgcagctgca gcgacgacga
     5461 acagcggccg caccagaccg ttcaagctcg cgccgagctg cgtcgagcgg cgcaccgcgt
     5521 agctcgcgag atacagcagt agacacagac gcgcgggctc ggatgctt
//
LOCUS       DATLEE010000779         2939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8128881, whole genome shotgun sequence.
ACCESSION   DATLEE010000779 DATLEE010000000
VERSION     DATLEE010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8128881"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /gene="sufB"
                     /locus_tag="VM692_17110"
     CDS             complement(<1..1323)
                     /gene="sufB"
                     /locus_tag="VM692_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HUQ53951.1"
                     /translation="MATESDTLSDLVKRKYRHGFVTDIEQETVPPGLSEDVIRLISAK
                     KGEPEFLTEWRLKAYRHWLTLTEPTWAHVRYTPIDYQAISYYAAPKNAGDGPKSLDEV
                     DPKLLETYDKLGIPLHERARLAGVAVDAVFDSVSVATTFKDKLAAAGVIFCSFSEAVK
                     NHPELVQRYLGSVVPYRDNYFATLNSAVFTDGSFVYVPKGVRCPMELSTYFRINAAKT
                     GQFERTLIIAEEGSHVSYLEGCTAPMRDENQLHAAVVELVALDNAQIKYSTVQNWYPG
                     DEHGRGGIYNFVTKRGDCRGANSKISWTQVETGSAITWKYPSCILRGENSVGEFYSVA
                     VANNYQQADTGTKMIHIGRNTRSTIVSKGIAAGHGQQSYRGLVQVSPNATGARNYSQC
                     DSLLIGDKCGAHTFPYIDVRNSSAIMEHEASTSKIGEDQIFYCRQRGLS"
     gene            complement(1339..1782)
                     /locus_tag="VM692_17115"
     CDS             complement(1339..1782)
                     /locus_tag="VM692_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011506533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HUQ53952.1"
                     /translation="MSKLTDYGTMVLAQLAASDAGWTTANQVADATHLGPPTVSKLLK
                     SLVHAGLVVSSRGVQGGYALARPAAAISAAEILDALEGPVAITECSSSNGGCDLESYC
                     RVGTAWQRINHSIRKALDGVSLADLQTRREPLPQLRTDLRAAAAR"
     gene            complement(1871..>2939)
                     /locus_tag="VM692_17120"
     CDS             complement(1871..>2939)
                     /locus_tag="VM692_17120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HUQ53953.1"
                     /translation="ISHVTQRHIARAAYDNQRNSIVSLAAMLAALVLGAASDMSNDAM
                     TGVVTASQALAAQRQINFTRGNEHEADRVGIELMSGAGFDPYGMASFFEKLGRRYGGQ
                     SQYVPALLQTHPVTSERTAEARDRARQLPATSHTSSFSYGLTKTRLIALNAATPEAAF
                     AAFRDKGDSDAPEDRYGLALASMRMGLNDNAEQLFRGLIQDYPNTMAFRVGQAEAMAA
                     SGATAAALAVYADAIRLFPRNVPLTISYAERLIAAGKPAEAHELLLDLLNNVPATAPQ
                     LRLIARAANAEGDVANAYFYMSYYYSSIGNLPLAIGQVRLALETPNANEVDRARFRSR
                     LDQLIEYLPDEQRELATSGSQ"
BASE COUNT          497 a          901 c          977 g          564 t
ORIGIN      
        1 ggaaaggccg cgctgccggc aatagaagat ctgatcctcg ccgatcttgg aagtcgaagc
       61 ctcgtgttcc atgatcgcag agctgttgcg cacatcgatg tacgggaagg tgtgcgcgcc
      121 gcacttgtcg ccgatgagca gcgagtcgca ctgcgagtag ttgcgcgcac cggtcgcgtt
      181 cggcgaaacc tgcacgaggc cgcggtagct ctgctggccg tgtccggcgg cgatgccctt
      241 cgagacgatc gtgctgcgcg tgttgcggcc gatgtggatc attttcgtgc cggtatcggc
      301 ttgctgatag ttgttcgcga cggcgacgga gtagaactcg ccgaccgagt tctcgccgcg
      361 caggatgcag ctcggatact tccacgtaat tgcggagccg gtttcgacct gcgtccacga
      421 gatcttcgaa ttcgcgccgc gacagtcgcc gcgcttcgtc acgaagttgt agatgccgcc
      481 gcggccgtgc tcgtcgccgg gataccagtt ctgcaccgtc gaatacttga tctgcgcgtt
      541 gtcgagcgcc acgagctcga cgacggcggc gtgcagctga ttttcgtcgc gcatcggtgc
      601 cgtgcagcct tcgaggtagc ttacgtggct gccttcctcg gcgatgatca aggtccgctc
      661 gaactgaccg gttttcgccg cgttgatgcg gaaataagtc gagagctcca tcgggcagcg
      721 cacgcctttc ggcacgtaga cgaacgaccc gtccgtgaac accgcggaat tcaatgtcgc
      781 gaagtaattg tcgcgatacg gcactacgga gccgagatag cgttggacga gctccgggtg
      841 attcttcacg gcttccgaaa acgagcagaa gatcacgccg gccgccgcaa gcttgtcctt
      901 gaaggtcgtg gcgacggaaa cgctgtcgaa gaccgcgtcg acggcgacgc cggcgagccg
      961 cgcgcgctcg tgcagcggaa taccgagctt gtcgtacgtc tcaagcaact tcgggtcgac
     1021 ttcgtcgagg ctcttcggac cgtcgccggc attcttcggc gccgcgtagt aggagatcgc
     1081 ttggtaatcg atcggcgtgt aacgcacgtg cgcccacgtc ggctccgtca acgtgagcca
     1141 atggcgatac gccttgaggc gccactccgt caggaactcg ggctcgcctt tcttcgccga
     1201 gatcaagcgg atgacgtcct cgctcaagcc cggcggaacg gtctcctgct cgatgtccgt
     1261 cacgaagccg tggcgatact tgcgcttgac gaggtcggat agggtgtcgg attcagtcgc
     1321 catgagtagt cgatttcctc accgtgccgc ggctgcgcgg aggtcggtgc gtagttgagg
     1381 caagggctcg cggcgcgtct gcaggtcggc gaggctgacg ccgtcgagcg ctttgcgaat
     1441 gctgtgattg atgcgctgcc acgccgtgcc gacccggcag tacgattcga ggtcgcagcc
     1501 gccattcgag gagctgcact ccgtgatcgc gaccggcccc tcgagcgcgt cgagaatctc
     1561 cgcggcgctg atggcggcgg cagggcgtgc aagggcataa ccgccctgca caccgcggct
     1621 cgagacgacc aagcccgcat gcacgagcga cttcagcaac ttgctgacgg tcggcggccc
     1681 gaggtgcgtg gcatcggcga cttgattggc cgtcgtccag cccgcgtcgc tggccgccag
     1741 ttgcgccagc accatggtgc cgtaatcggt caacttgctc atgcgcagca tggaggcgtt
     1801 tcctcgggtc ctttgatagt ggactatttt agtcctattt gagcccgcgg caacgggcga
     1861 gcgaaagaat ctactgcgat ccggaggttg cgagctcgcg ctgctcgtcg ggcaagtatt
     1921 cgatgagctg gtcgaggcgg gagcgaaacc gcgcgcggtc gacttcgttc gcgttcgggg
     1981 tctcgagcgc caagcgcacc tgaccgatcg cgagcggcag attgccgatc gacgagtagt
     2041 agtacgacat atagaaatac gcgttggcga cgtcgccttc ggcattggcc gcgcgcgcga
     2101 tcaaccgcag ctgcggcgcg gtcgccggca cgttgttcag cagatcgagc aggagctcgt
     2161 gcgcttcagc gggcttgccg gccgcgatca acctctcggc atagctgatc gtcagcggca
     2221 cgtttctcgg gaacaagcga attgcgtccg catagaccgc tagcgccgcg gcggtcgcgc
     2281 cgctcgctgc catcgcctcg gcctgtccga cacggaaggc catcgtgttg gggtagtcct
     2341 ggatcaggcc gcgaaacaac tgctcggcgt tgtcgttgag ccccatgcgc atcgaggcca
     2401 aggcgagacc gtagcgatcc tcgggcgcat cgctgtcgcc cttgtcacga aaggcggcga
     2461 aagcagcctc cggcgtcgcc gcgttcaggg ctatcaagcg cgttttcgtc agaccgtagg
     2521 agaagctgct cgtatgcgac gttgccggca gctgtcgcgc gcggtcgcgt gcctcggccg
     2581 tgcgctccga ggtcacgggg tgcgtttgca agagcgccgg cacgtattgt gactgcccgc
     2641 cgtagcggcg gccgagcttc tcgaagaagc tcgccatgcc atacggatcg aagccggcac
     2701 ccgacatcaa ctcgatgccg acgcgatctg cctcgtgctc gttcccgcga gtgaaattga
     2761 tctgccgctg tgcggcgagc gcttgcgacg cggtgacgac gccggtcatt gcgtcgttcg
     2821 acatgtcgga ggcggcaccg agcacaaggg cggcgagcat ggccgccagc gacacaatgc
     2881 tattgcgctg attgtcgtaa gcggcgcgcg cgatgtgtcg ttgcgtgaca tgcgagatc
//
LOCUS       DATLEE010000780         3337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10364397, whole genome shotgun sequence.
ACCESSION   DATLEE010000780 DATLEE010000000
VERSION     DATLEE010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10364397"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VM692_17125"
     CDS             complement(<1..922)
                     /locus_tag="VM692_17125"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HUQ53954.1"
                     /translation="MRAKRVSLLAAAAVALAAPLAAQSQDGPLVLTGTQATFSLTVAL
                     TAGTPIHVQNVPADGRQLNLLKDYIQRRTDALEPTFDSAAAVVTLTNALPGDPLYRFA
                     REANIRIVDIEAAIPWSLDAPGVALADTPESSVDWGSDPDVAETAVAPYFWLSVSNSI
                     RMADIIAQDLEALFPDSAAKVAANLQTLKRSLLDLRGEYQGRLIESGADVVFALTSDF
                     VYLTNDMGLFVDGYFVKQDVRWTDKDLAALTKHLRDNGIKVVLHKWQPSEAIQNAISA
                     AGAKLVVLDAGDPGVVVDRALAADGLQQILR"
     gene            complement(919..1815)
                     /locus_tag="VM692_17130"
     CDS             complement(919..1815)
                     /locus_tag="VM692_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HUQ53955.1"
                     /translation="MFEVPRQFIMDLANDNVIHYTFSYAFVVNGFLCALVIGPLLGGI
                     GTMVVAKRMAFFSQAVGNAALTGVAIGVIIGESYTAPYVSMFSFCLLFGLVLNFARNR
                     TKMSADTLIGVFLSISLAIGATALLWVSAKVNTHILDTVMFGSILTVDDTDMTVLLIT
                     TVLLAVTALPLFNHMLLASLNPSLAHVRGVRVHLLEYVFVMLLTILTVACVKIIGAVL
                     VEALLLIPAAAARNLNKSIRGFVLWSIAFSTISCIAGIYAPMRFDLPLPSGGAIILAS
                     AMIFVVTLLLRMALPRYREASL"
     gene            complement(1821..2546)
                     /locus_tag="VM692_17135"
     CDS             complement(1821..2546)
                     /locus_tag="VM692_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015850877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HUQ53956.1"
                     /translation="MQGPSIEFDRVNLTLGATSILQDVSFKVRAGTIHCIVGANGGGK
                     TSLVRSLLGQMPHSGRINIEWHGSQVVGYVPQSLDFDKSLPITVLDFLAMTTQRRPVF
                     LGVARARRLEIDKTLARLGLAGKLNAKLGSLSGGERQRVLLAQALIPEPALLVLDEPT
                     TGLDVAGREVLERTIIEFVKAGGTAVWINHDITQVNEIADAMTYIDRKVLLDGVPREV
                     LATGAAEHLFPTLALSLREAVSS"
     gene            complement(2551..>3337)
                     /locus_tag="VM692_17140"
     CDS             complement(2551..>3337)
                     /locus_tag="VM692_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HUQ53957.1"
                     /translation="NIVGDRAEIIPLIDADANPHGYAPQPADMIRITSMDVMIVNGIG
                     HDTWAFEILDAAGLTDKLPLIYANDGVSLIPIAGDPSGAKVVNPHTFISTTAAIQQVY
                     TIARKLGELDPENATYFRDNGRKYALQIRKLRAEFDAQIAGADLTKFRCATMHSGYDY
                     ILQELGLQVTAVIEPRHGVEPTARQLAETIDRIKGANVNVLFAEKYFASKLSDTIREA
                     TGVKMYSISHISSGEYTPTKFIDEMRENLTTLATAIRESAQGA"
BASE COUNT          596 a         1063 c         1113 g          565 t
ORIGIN      
        1 tgcgcaggat ttgctgcaag ccgtcggccg cgagcgcgcg atcgacgacg acgccggggt
       61 cgccggcatc gagcacgacg agcttcgcgc cagccgcgct aatggcgttc tgaatcgctt
      121 cgctcggctg ccacttgtgc agcacgacct tgatgccgtt gtcgcgcagg tgcttcgtca
      181 gcgcggcgag atccttgtcc gtccagcgca cgtcctgctt gacgaagtag ccgtcgacga
      241 agaggcccat gtcgttcgtc agatagacga agtcgctcgt caacgcgaag acgacgtccg
      301 cgccgctctc gatgagccgc ccttggtatt cgccgcgcag atcgagcagc gagcgcttca
      361 acgtttgcag gttcgccgcg accttggcgg ccgagtccgg gaacagcgcc tcgagatctt
      421 gcgcgatgat gtcggccatg cggatcgagt tcgagacgct gagccagaag tacggcgcga
      481 cggccgtctc ggcgacgtcc gggtcgctgc cccaatcgac gctcgactcc ggcgtgtcgg
      541 cgagcgcaac gcccggcgcg tcgagcgacc acggaatcgc ggcctcgatg tcgacgatgc
      601 gaatgttggc ctcgcgcgcg aagcgataca gcggatcgcc gggcagcgcg ttcgtcaacg
      661 tcacgacggc ggccgccgag tcgaaggtcg gctccagcgc gtcggtgcgc cgctggatgt
      721 aatctttgag caagttcagc tggcggccgt cggccggcac gttctgcacg tggatcggcg
      781 tgccggccgt cagcgcgacg gtcagcgaga acgtggcctg cgtgcccgtc agcacgagcg
      841 ggccgtcttg ggactgcgcg gccaatggcg cggccagcgc gacggcggcg gccgccaaca
      901 gcgaaactcg cttcgctctc atagactcgc ctcccgataa cgaggcagcg ccatgcgcag
      961 cagcagcgtg acgacgaaga tcatggccga cgcgaggatg atcgcgccgc ccgacggcag
     1021 cggcagatcg aagcgcatcg gcgcgtagat gccggcgatg cagctgatcg tcgagaacgc
     1081 gatgctccag agcacgaagc cgcggatcga cttgttgaga ttgcgcgccg cagccgccgg
     1141 aatcagcagc agggcctcga cgagcacggc gccgatgatc ttcacgcaag ctaccgtcag
     1201 gatcgtcagc agcatcacga acacgtactc gagcaggtgc acgcgcacgc cgcgtacgtg
     1261 cgcgaggctc gggttcaaac tcgcgaggag catgtggttg aacaacggca aagccgtgac
     1321 ggccaagaga acggtcgtga tcagcaacac ggtcatgtcg gtgtcgtcga cggtcaagat
     1381 cgagccgaac atcaccgtgt cgaggatgtg cgtgttgacc ttcgccgaca cccagagcag
     1441 cgccgtggcg ccgatcgcga gcgagatcga caggaacacg ccgatcaacg tatccgccga
     1501 cattttcgtg cggttgcgcg cgaagttcag caccaagccg aacagcaggc agaagctgaa
     1561 catcgacacg tacggcgccg tgtatgactc gccgatgatg accccgatgg cgacccccgt
     1621 cagcgccgcg ttgccgacgg cctgcgagaa gaacgccatg cgctttgcga cgaccatcgt
     1681 gccgatgccg ccgagcaacg gcccgatgac gagcgcgcac aggaagccgt tgacgacgaa
     1741 cgcgtacgag aacgtgtaat gaatgacgtt gtcgttcgcc agatccatga tgaactggcg
     1801 cggcacttcg aacagcgcgg tcatgacgac accgcctcgc gcagcgacaa agccagcgtc
     1861 gggaacaggt gttccgcggc gccggtcgcg agcacctcgc gcggcacgcc gtcgagcaac
     1921 actttccgat cgatgtaggt catggcgtcg gcgatctcgt tgacctgcgt gatgtcgtga
     1981 ttgatccaga cggccgtgcc gccggctttg acgaactcga tgatggtgcg ctcgaggacc
     2041 tcgcggcccg cgacgtcgag gcccgtcgtc ggctcgtcga gcacgagcaa cgccggctcg
     2101 gggatcaaag cctgcgccag cagcacgcgt tgccgttcgc cgcccgacag gctgccgagc
     2161 ttcgcgttga gcttgcctgc caatccgagc cgcgccagcg tcttgtcgat ctcgaggcgg
     2221 cgcgcacgcg cgacgccgag gaataccggc cggcgctgcg tcgtcatggc caggaaatcg
     2281 agcaccgtga tcggcaggct cttgtcgaag tcgagcgact gcggcacgta gcctacgacc
     2341 tggctgccgt gccactcgat gttgatgcgg cccgaatgag gcatctgccc gagcagcgac
     2401 cgcacgagcg acgtcttgcc gccgccgttg gcgccgacga tgcaatggat cgtgccggcg
     2461 cgcaccttga agctgacgtc ttgcagaatc gacgtggcgc cgagcgtcaa gttgacgcgg
     2521 tcgaattcga tagaaggacc ttgcatgatc tcaagcgcct tgcgcggact cgcggatggc
     2581 cgtcgcgagc gtcgtcaagt tctcgcgcat ctcgtcgatg aacttcgtcg gcgtgtactc
     2641 gccgctgctg atgtgcgaga tcgagtacat cttgacgccc gtggcttcgc gaatcgtgtc
     2701 cgacagcttg ctcgcgaaat acttctcggc gaacaacacg ttgacgttcg cgcccttgat
     2761 gcgatcgatc gtctccgcga gctgacgcgc cgtcggctcg acgccgtgcc gcggctcgat
     2821 gacggccgtt acctggagcc caagctcctg aaggatatag tcgtaaccgc tgtgcatcgt
     2881 cgcgcagcgg aacttcgtca gatccgcgcc tgcgatctgc gcgtcgaact cggcgcgcag
     2941 cttgcggatc tgcagcgcgt acttgcggcc gttgtcgcgg aagtacgtgg cgttctcggg
     3001 gtcgagctcg ccgagcttgc gagcgatcgt gtagacctgc tgaatcgccg ccgtcgtcga
     3061 gatgaacgtg tgcggattga cgaccttggc gccgctgggg tcgccggcga tcggaatcag
     3121 cgacacgccg tcgttggcgt agatcaacgg cagcttgtct gtcaggcccg cggcatcgag
     3181 gatctcgaac gcccaggtgt cgtggccgat gccgttgacg atcatgacgt ccatcgacgt
     3241 gatgcggatc atgtcggccg gctgcggcgc gtaaccgtgc gggttcgcgt cggcatcgat
     3301 cagcggaatg atctcggcgc ggtcgccgac gatgttg
//
LOCUS       DATLEE010000781         2980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8137722, whole genome shotgun sequence.
ACCESSION   DATLEE010000781 DATLEE010000000
VERSION     DATLEE010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8137722"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VM692_17145"
     CDS             complement(<1..231)
                     /locus_tag="VM692_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HUQ53958.1"
                     /translation="MRIDWNEYQPSGFYDELFAAPGTARPSAAQLADYLSSLGIRALA
                     ERQADAERAIVEMGITFTVYTEGQNIDRAWPFD"
     gene            complement(234..1736)
                     /locus_tag="VM692_17150"
     CDS             complement(234..1736)
                     /locus_tag="VM692_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HUQ53959.1"
                     /translation="MNAVLGTPMSSSAKRFVAFVGVGLVVGSATAFAQAQSLPSPVDT
                     TGWTTADDHRNMLEQLGIRQLRPGPTADASKPNAANYDEARANPFPVLPDALTLRSGA
                     KVTTADAWWGQRRAEILEDFERDVVGRVPPVVPGVTWSVAATREWEIGGRRVVGRQLV
                     GRVDNRAYPAIDVEICMTLVTPAEAQSVPVMIMLGGGNLPAEAIEPEALPGACRPPAR
                     PAGAPPLPAPPVGGDPPATQQLIAAGWGFASLRPTSVQADNGAGLTQGIIGLVNEGRP
                     RKPDDWGALRAWAWGASRGLDYLIAEGETQGVVDAKRVGVEGVSRYGKAALVAMAFDT
                     RFAVVLVGSSGEGGAKLHRRNFGEAVENLTASGEYHWMAGNFLKYGAADATFGSKNAG
                     DLPVDAHELIALCAPRPTFLSYGVPEKGDALWLDQQGSFMAAVAAGPVFRLLGAHDLG
                     TSGDYRHEKMPPVGVGLLNGELAWRQHDGGHTDGPNWRYFIPWANEQLAR"
     gene            1827..>2980
                     /locus_tag="VM692_17155"
     CDS             1827..>2980
                     /locus_tag="VM692_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HUQ53960.1"
                     /translation="MQPNILAQPALAAEGRQKIDWVRRNMPILRHLEERFAAERPFAG
                     KRIAVCIHLEAKTAYLALVLRAGGAAVAVTGSNPESTKDDVVAALATEGLHVYARHGA
                     PPDEMRRYMGVALEIAPHVVIDDGGDLVELLHESHRGHGKEMLGACEETTSGVLRARA
                     RAKAGQLRFPVVLVNDARCKYLFDNVHGTGQSVWDGVMRATNLVLAGKTVVIVGYGWC
                     GKGCAMRAQGLGANVVVCEVDPIKAADALMNGCRVLPLAEACRVADVVLTVTGVRHTL
                     RAEHFALLKSNVLLANAGHFWEEIDVAALAKAAVERKPLRANVEGFRLQNGSWLNLLG
                     GGNIVNISCADGHPAEIMDMSFALQALAARHVVEQAASLRPDCYPVPASI"
BASE COUNT          471 a         1049 c         1006 g          454 t
ORIGIN      
        1 gtcgaacggc cacgcgcgat cgatgttctg gccttccgtg tagaccgtga acgtgatgcc
       61 catctcgacg atcgcgcgct cggcatcggc ttgccgctcg gcgagcgcac ggatgccgag
      121 cgacgacaga tagtccgcga gctgcgcagc ggacggccta gccgtgccgg gcgcggcaaa
      181 cagctcgtcg taaaacccgc tgggttggta ttcgttccag tcgatgcgca tgatcaacgc
      241 gcgagctgct cgtttgccca cgggatgaag tagcgccaat tcggaccgtc cgtgtggccg
      301 ccatcgtgct gtcgccacgc gagctcgccg ttcaataggc cgacgccgac cggcggcatc
      361 ttctcgtggc gataatcgcc ggacgtgccc aagtcgtgcg cgccgagcaa acgaaacacg
      421 ggccccgccg cgacggccgc catgaagctg ccttgctgat cgagccagag cgcgtcgccc
      481 ttctcgggca cgccgtaact caagaacgtc ggccgcggcg cgcagagcgc gatgagctcg
      541 tgcgcgtcga cgggcagatc gccggcgttc ttgctgccga acgtcgcatc ggcagcaccg
      601 tacttcagga agttgccggc catccagtga tactcgccgc tcgccgtcag gttctcgacg
      661 gcctcgccga agttgcgccg gtgcagcttc gctccgccct cgcccgaaga gccgacgagc
      721 acgacggcga agcgcgtgtc gaacgccatc gcgacgagcg cggccttgcc gtagcgcgac
      781 acgccttcga cgccgacgcg cttcgcgtcg acgacgccct gcgtttcccc ctcggcaatc
      841 agataatcga gaccgcgcga tgcgccccac gcccacgcgc gtagcgcgcc ccagtcgtcg
      901 ggtttgcgcg ggcggccctc gttgacgagc ccgatgatgc cctgcgtcaa accggcgccg
      961 ttgtcggcct gcacgctcgt cggccgcaac gacgcgaagc cccagcccgc cgcgatcaat
     1021 tgctgcgtgg ccggcggatc gccgccaacg ggcggcgcgg gcaacggcgg cgctccggcc
     1081 ggccgcgccg gcgggcgaca cgcgccgggc agcgcctcgg gctcgatcgc ttcggcgggc
     1141 aagttgccgc cgccgagcat gatcatgacg ggcacgctct gcgcctccgc cggcgtcacg
     1201 agcgtcatgc agatctcgac gtcgatcgcg ggatacgccc ggttgtcgac gcggccgacg
     1261 agctgccgcc cgacgacgcg gcgaccgccg atctcccact cgcgcgtcgc cgcaaccgac
     1321 cacgtgacgc cggggacaac gggcggcacg cggccaacga cgtcgcgctc gaagtcctcg
     1381 aggatttcgg cgcggcgttg gccccaccag gcgtcggccg tcgtgacttt ggcgccgctg
     1441 cgcagcgtca acgcgtcggg caagaccgga aacggattcg ctcgcgcttc gtcgtagttc
     1501 gccgcgttcg gcttcgacgc gtcggccgtc gggccgggcc gcaattgccg aatgccgagc
     1561 tgctcgagca tgttgcggtg atcgtcggcc gtcgtccaac ctgtcgtgtc gacgggtgac
     1621 ggcaacgatt gcgcctgcgc gaacgcggtc gcgcttccga cgacgagccc tactccaacg
     1681 aaagcaacga agcgcttggc gctgctcgac atcggtgtcc ccaggacagc attcatgcga
     1741 cgcgccaaag gtaccgcggc ccgtgtgcgg cggatagagt ttcgagtagc ctcacgcctt
     1801 cgaacaacgc gttagagtcg taagcaatgc agccgaacat cctcgcccag cccgcgctcg
     1861 ccgccgaagg gcgccagaag atcgactggg tgcggcgcaa catgccgatt ctgcgccacc
     1921 tcgaggagcg cttcgccgcc gagcggccgt ttgccgggaa gcgaatcgcg gtttgcattc
     1981 acctcgaagc gaagaccgcc tatctcgcgc tcgtgctgcg cgccggcggc gccgcggtcg
     2041 cggtcacggg cagcaatccc gagtcgacga aggacgacgt cgtcgcggcg ctcgcgaccg
     2101 aagggctgca cgtgtatgcg cggcacggcg cgccgcccga cgagatgcgc cgctacatgg
     2161 gcgttgcgct cgagattgcg ccgcacgtcg tcatcgacga cggcggcgat ctcgtcgagc
     2221 tgctgcacga atcgcatcgc ggccacggca aggagatgct cggcgcttgc gaggagacga
     2281 cgtcgggcgt gctgcgcgcc cgcgcgcgag ccaaggccgg gcagctgcgc ttccccgtcg
     2341 tgctcgtcaa cgacgcgcgc tgcaagtatt tgttcgacaa cgtgcacggc acgggacaat
     2401 cggtctggga cggcgtcatg cgcgccacga atctcgtgct ggccggcaag accgtcgtca
     2461 tcgtcggcta cggctggtgc ggcaaaggct gcgccatgcg cgcgcaaggg ctcggcgcca
     2521 acgtcgtcgt ctgcgaagtc gaccccatca aggccgccga cgcgctgatg aacggctgcc
     2581 gcgtgttgcc gttggccgaa gcgtgccgcg tcgccgacgt cgtgctgacg gttaccggcg
     2641 tgcgccatac gctgcgcgcc gagcacttcg cgctgctcaa gagcaacgtg ctgctcgcga
     2701 acgccgggca cttctgggaa gagatcgacg tcgctgcgct cgcgaaggcg gctgtcgaac
     2761 gcaagccgct gcgcgcgaac gtcgaagggt ttcggctgca aaacggctcg tggctgaacc
     2821 tgctcggcgg cggcaacatc gtcaacatct cgtgcgccga cggccacccc gccgagatca
     2881 tggacatgag cttcgcgctg caagcgcttg cggcgcggca cgtcgtcgag caagccgcgt
     2941 cgttacggcc cgactgctat cccgtgcccg cgtcgatcga
//
LOCUS       DATLEE010000782         4882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6575048, whole genome shotgun sequence.
ACCESSION   DATLEE010000782 DATLEE010000000
VERSION     DATLEE010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6575048"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..2582)
                     /locus_tag="VM692_17160"
     CDS             complement(78..2582)
                     /locus_tag="VM692_17160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ53961.1"
                     /translation="MSGLFDALGRDLKFTRRILGKNLAVTVTAIVTLALGIAASTVIY
                     TVVDAVLLQPLGYQDSGDIYRVYTVDPVGLPRGTTGPPQIDPIADDESDQIQAAFFGF
                     SFEQSVVNDAGTAFAVNEFRASDEFFDVFTEPLLMGREFQPREDFRNTILSYRTWRDV
                     FGADPNIIGKPIRVGSGPLNVIGVAADGFEFPVGTAMWTKVFTGPPGQPLQLYNQSGY
                     VRAQPGLSPAQVQAQLDVFAARIAKWPDGRKLEFVARPLLEDVVGDLRPTLLIVSGAT
                     ALLLLIACINVANLLLARGVVRASEFALREAMGARRWRMFRQLMTESFVLCTLGGALG
                     LGVALGALQLVKAIGPADLPRFSTLSIDANMFLFAAACVLFVTFIVGLAPALRVSRGG
                     LIGLINTGGRSAGTARGRNRTFGSLVIAEIALAVVLVIGAGLLVRSYSQLASADPGFD
                     PRRMVTLVLNVTGRTQFQNMRFDQERQAVVYDGPGMAGVAQFYQELIRRIDELPGVAS
                     AGATFAAPLNHGLFPIVLWPNPVVGSSAAPTDQLAYVFPVSPEFFTTLGIRPLTGRLL
                     EPTDRRGAPGVTVVNEAYARAYLDGQNVVGRRVALLGGNWQPGSPGFGQVGEQTVNEA
                     EIVGVIPDIKQANLQDRVQPAVYLSQEHLTMRKMAIVVRSENDDPAALIPSIRRELEA
                     MDSTIPPTFAVYSDVVAASLARHKLGALVLLVFGVVSLTLAAVGTYGLISFSVNQRFN
                     EIAVRSAFGAERGNLLQLFVKRALQLAGIGIVIGVVGAIAMRQVVASQLYEISAFDPW
                     VLAIVPLTMLAVTVVASYVPARRASRIDVTSALRQN"
     gene            complement(3001..>4882)
                     /locus_tag="VM692_17165"
     CDS             complement(3001..>4882)
                     /locus_tag="VM692_17165"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUQ53962.1"
                     /translation="FIVGQSPDVWLPLSLDTPAVTSFTDFQPIEVLARLPAEATPATV
                     KPELDTLLARALAEGPRSFFGDEPVTTAIESPADRFGGEARDALLVLLGAVALVLLVA
                     CSNVANLLLARGASRARELSLRSALGASSWRLVRALVVECLLLAGMAGVAGAALGWLT
                     LSVLVRVRPATLSQLNEVQLDGPVLAFTFGVSVATALLFGLAPALQLTSRKLGDALRH
                     GASGVVRGGGGARVRKLLVAGQMALSVVLLVSAGLLVRSVINLQQIDVGFDTDKLFTA
                     RLSLPRARYQAPASRDALAEQMLERALPSVAGVTQAHVGPVGGLFGSGTLEIRGVTLS
                     DADSRGPQAFNYVRANFFDVLGIRMLEGRTFTADELQNGRAIIVNRAAEQRFWPDGRA
                     LGSELRLGQGQDWSAVVGVVDTVAAGGFMSSTATTLPQLYWPYSAMRAPTTIGATPTV
                     LLIARARSEPADAIAALRGATRELDPEIAIREVLMTEMELTRTIDGPRFNMTLLTAFA
                     FVGLVLAAVGLAAVIGYEVTERTHELGIRMALGARTANVRRLAMKHGLQPAFIGVVIG
                     VVGALGATQLAASLLYGVAPRDPLTFVGVVALLSAVAVGASWLPARRATRVDPIAALR
                     SE"
BASE COUNT          924 a         1640 c         1619 g          699 t
ORIGIN      
        1 cagatcgcgg ccgagcgcgt cgagccagcc ggaggtcagc gtcgtcattc aacctctcct
       61 gaattacgcg cgcgctgcta gttctgccgc agcgcggacg taacgtcgat gcgcgacgcc
      121 ctgcgcgccg gcacgtacga agcgaccacc gtgaccgcga gcatcgtcag cggcacgatc
      181 gccagcaccc acgggtcgaa cgcgctgatc tcatagagct ggcttgcgac gacttgccgc
      241 atcgcgatcg cgccgacgac accgatgacg atgccgatcc ccgcaagctg cagcgcacgc
      301 ttcacgaaca gctgcaacaa attgccgcgt tcggcgccga acgccgagcg caccgcaatc
      361 tcgttgaacc gctgattgac cgagaacgag atcaggccat aggtgccgac ggcggcgagc
      421 gtcagcgaga cgacgccgaa gaccagcagc acgagagcgc ccagcttgtg ccgtgccaac
      481 gacgcggcga cgacgtcgga gtaaacggca aacgtggggg gaatcgtcga gtccatcgcc
      541 tcgagctcgc ggcgaatcga gggaatcaat gcggccgggt cgtcgttctc cgagcggacg
      601 acgatcgcca tcttgcgcat ggtcaggtgt tcctgcgaca agtagaccgc cggttgcacg
      661 cggtcctgga gattggcctg cttgatgtct gggatcacgc cgacgatctc ggcctcgttg
      721 accgtctgct cgccgacctg accgaacccg ggcgacccgg gctgccaatt cccgcccagc
      781 aacgcgacgc ggcggccgac gacgttctgc ccatcgagat acgcgcgcgc gtacgcttcg
      841 ttgacgaccg tgacgccggg cgcgccgcgc cgatcggtcg gctcgagcaa ccggccggtg
      901 agcggccgga tgccaagcgt cgtgaagaac tccggcgaaa cgggaaagac gtaggcgagc
      961 tggtcggtgg gggctgcgct gctacccacg acggggttgg gccagagaac gatcgggaac
     1021 aacccgtggt tcagcggcgc ggcgaacgtt gcgccggccg aggcgactcc cggcagctcg
     1081 tcgatgcggc gtatgagctc ctggtagaac tgggcgacgc ccgccatgcc gggtccgtcg
     1141 taaacgacgg cctgccgttc ctgatcgaag cgcatgttct ggaactgggt gcgccccgtg
     1201 acgttcagca ccagcgtgac catacgccgc ggatcgaaac cggggtcggc cgaagcgagc
     1261 tgggaatagc tgcgcacgag cagccccgca ccgatcacga gcacgacggc cagggcgatc
     1321 tcggcgatca cgagcgagcc gaaagttcgg ttccgccctc gcgccgtgcc ggccgagcgt
     1381 ccgcccgtat tgatcaaacc gatcaagccg ccgcgcgaca cgcgcagcgc aggcgcgagc
     1441 ccgacgatga acgttacgaa caacacgcac gcggccgcga acaaaaacat gtttgcgtcg
     1501 atcgacagcg tggagaaccg cggcagatcc gcgggtccga tggccttcac gagctggagc
     1561 gcgccgagcg cgacaccgag accgagcgct ccgccgagcg tgcaaagaac gaagctctcg
     1621 gtcatgagct gtcggaacat ccgccaacgg cgcgccccca tcgcctctcg cagcgcgaac
     1681 tcgctcgcgc gcaccacgcc gcgcgcgagc agcaaattcg ccacgttgat gcaggcgatc
     1741 agcagcagga gcgccgtcgc cccgctgacg atcagcaacg tgggccgcaa atcgccgacg
     1801 acgtcctcga gcaacggccg cgccacgaac tcgagcttgc ggccgtccgg ccatttcgcg
     1861 atgcgtgccg cgaagacgtc gagctgcgcc tggacctgcg ccggcgagag gccgggctgt
     1921 gcgcgcacgt agcccgactg gttgtagagc tgtagtggct gaccgggcgg ccccgtgaag
     1981 accttggtcc acatggccgt gccgaccgga aactcgaagc catcggccgc tacgccgatg
     2041 acgttcaggg ggccgctgcc aacacgaatt ggcttgccga tgatgttggg atcggcgccg
     2101 aacacgtcgc gccacgttcg ataactcagg atcgtgttgc ggaagtcctc gcgaggctgg
     2161 aattcccgcc ccatgagcaa cggctcggtg aagacgtcga agaactcgtc cgaagcgcgg
     2221 aattcgttga ccgcgaacgc ggtgccggcg tcgttgacga ccgattgctc gaacgagaag
     2281 ccgaagaacg cggcctgaat ttggtcggac tcatcgtcgg cgatcgggtc gatctgcgga
     2341 gggcccgtcg tgccgcgcgg caggccgacg gggtcgacgg tataaacacg atagatgtcg
     2401 cccgagtcct ggtagcccag cggctggagc agcaccgcgt cgacgaccgt gtaaatcacg
     2461 gtgctcgctg cgataccgag cgccaacgtc acgatcgcgg tcaccgtgac cgccaggttc
     2521 ttgccgagaa tgcgccgcgt aaatttcaaa tcgcggccga gcgcgtcgaa taagccggac
     2581 atctgatgac agcctccccc gaagctggtt ggcgtgcgct ttgcgcttgt cgttcaacgg
     2641 gcgcgggccc gccgtaaaga taaaaccgtg tactctcgga cgataaacct tgccgtgttc
     2701 ggcacgccga aagatcacaa gttccggcgc ccccgacgga ccgatcggga cgctttgcgc
     2761 ccgccgcgga tgttccactt tagtggaatg ccgctgcgag gctcacgcgc gtacggttgg
     2821 cgctgaccca cctttggggc cgacaggacg acccgggcga gcccccgttc aaaggcgtga
     2881 ttgcgcagtg gggccagata ccgaagttca tccgtgacat catcacggcc atgggcggca
     2941 accccggcgt ctcttgcaac ccgaccaagg ccacgggagt agaaccgggc tagcgtgcgg
     3001 tcattcggaa cgcagcgcgg cgatcggatc gacgcgcgta gcgcgccgcg ccgggagcca
     3061 actcgcaccg acggcgacgg ccgagagcag cgcgacgaca ccgacgaacg tcaacggatc
     3121 gcgcggcgcg acgccgtaca gcaagctggc ggcgagctgc gttgcgccaa gggcgccgac
     3181 gacgccgatc acgacgccaa tgaacgccgg ttgcaggccg tgtttcatcg cgaggcggcg
     3241 tacgttcgcg gtgcgcgcgc cgagcgccat gcggatgccg agctcgtgcg tgcgctccgt
     3301 gacttcgtag ccgatgacgg ctgcaagacc gacggccgcg agcaccaggc cgacgaaggc
     3361 gaacgccgtc agcagcgtca tgttgaagcg cgggccgtcg atcgtgcgag tgagctccat
     3421 ttccgtcatc agcacttctc tgatcgcgat ttcggggtcg agctcgcgag tcgcgcctcg
     3481 tagcgctgcg atggcatcgg ccggctcgct gcgggcgcgc gcaatcagca acacagtcgg
     3541 cgtcgcccct atggtagtgg gcgctcgcat cgcagagtac ggccaataaa gctgcggcaa
     3601 ggtcgtggcc gtgctgctca tgaagccgcc cgccgcaacc gtgtcgacga cgccgacaac
     3661 agcggaccaa tcttgtccct gtcccaggcg cagctcgctg ccgagcgccc ttccgtcggg
     3721 ccagaagcgc tgctcggccg cgcgattgac gatgatcgct cggccgtttt gcagctcgtc
     3781 ggccgtgaac gtgcggcctt cgagcatgcg aattccgagc acgtcgaaga aattcgcccg
     3841 cacgtagttg aacgcttggg ggccgcgact atcggcgtcg ctgagcgtca ctccgcggat
     3901 ctcgagcgtg ccgctgccga acagcccgcc gacgggtccg acatgggctt gcgtgacacc
     3961 ggcgacactg ggcaaagccc gctcgagcat ctgctcggcc agcgcgtcgc ggctagccgg
     4021 cgcctggtaa cgggcacgcg gcagcgacag gcgcgcagta aatagcttgt cggtatcgaa
     4081 gccgacatca atctgctgca ggttgatgac gctgcgcacc agtaagccgg cactgacgag
     4141 cagcaccacg gatagagcca tctgccccgc gacgagcagc ttgcggacgc gcgcgccgcc
     4201 gccaccccgt acgacgcccg aggcgccatg gcgcagggcg tcgccaagtt tgcgcgacgt
     4261 cagctgcaac gcaggcgcga gaccgaacag caaagccgtt gcgaccgaga cgccgaaggt
     4321 gaaagcgagg accggaccgt cgagctgtac ctcgttcaat tgggacagcg tcgccgggcg
     4381 tacccgcacc aagacgctca acgtgagcca ccccaacgcg gcgcccgcga cgccggccat
     4441 accggccagc agcagacact cgacgacgag cgcgcgcacg aggcgccaac tgctggcacc
     4501 gagcgccgag cgcaacgaca gctcgcgtgc gcgcgaggca cctcgtgcca gcaacaagtt
     4561 cgccacgttc gagcaggcga cgagcagcac gagcgcgacg gcacccagca acacgagtag
     4621 ggcgtcgcgc gcctcgccgc cgaatcgatc cgccggactc tcgattgcgg tcgttacggg
     4681 ttcgtctccg aaaaatgacc gcggcccctc ggccagtgcg cgcgccagca gcgtgtcgag
     4741 ctccggcttg acggtggctg gagttgcctc cgccggcaac cgcgccaaca cttcgatcgg
     4801 ttggaaatcc gtgaaagatg tcaccgccgg cgtatcgagc gacagcggca accagacgtc
     4861 gggcgactgt ccgacgatga ac
//
LOCUS       DATLEE010000783         3732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10380148, whole genome shotgun sequence.
ACCESSION   DATLEE010000783 DATLEE010000000
VERSION     DATLEE010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10380148"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VM692_17170"
     CDS             <1..443
                     /locus_tag="VM692_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53963.1"
                     /translation="FAQSAALLAASVTAWPAWPARAALAAVAPLDDATIALVDRDLPG
                     GAAFARAARQRGMTTLEFAADVAELWMRELEPRLRNGPIRIAGHASAATLFCLDLLAR
                     DFGARTVRRVDAATAVSFVISQYSGPRAALAPAAVRAQWRHSYG"
     gene            436..2034
                     /locus_tag="VM692_17175"
     CDS             436..2034
                     /locus_tag="VM692_17175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUQ53964.1"
                     /translation="MASPPPGLSERDFAAALRDLESVVGREWLFSSDEDVLLYRDGYS
                     PLWGEAEERKASAAVAPSSVEQVQGVVKVANQYGLPLYPISTGRNLTYGGAAPAYSGS
                     VVVDLKRMNRILDVSARDKTCLVEPGVSYFDLYRHLRTNKIPLWIDTADPGWGGLIGN
                     ALDRGGGYTDIEFRDHFDAHCGMEIVLPTGELLRTGMNASPQAKTGQLFKYGLGPWVD
                     GIFSQSNFGIVTKMGFWLMQEPEAALQVSVTVPKRRDVVPLVDTLHSLMCAHVVPSQV
                     NVVSPVMAGNNAEMLRLRGSGGASDEQWDRYAATAGGAPFWAATFVYYGARNVIAAQW
                     QHTKDRLGGIAGVGFQETASYTFPLSDEQVEAVADKARYGIPSLNLFGSRTAPGTPPL
                     EGHMDFSPMIPPHGEELIALTDYTGKVYADQDFTPSFVGGLMFHPRTMICFHAVPTFR
                     NAADNARTRKLFETLVRGCAERGWPIYRIHPVFQKLGMEMFGFNSGALHRLHETLKDA
                     IDPNGIVSPGRYDIWPKRLRGSRA"
     gene            2031..2432
                     /locus_tag="VM692_17180"
     CDS             2031..2432
                     /locus_tag="VM692_17180"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUQ53965.1"
                     /translation="MKHTQLVAVALLGVAATAAAQDPQGPVPIAAWSRTTAAVETQGA
                     PVERGAAVFNNWCSACHSRGPQNAPGTSSLQMKYQGSVPAALEDRRDLTPEVVKTFVR
                     NGVAMMAPFRKVEVSDADLDALAAYLTRSRP"
     gene            2429..3046
                     /locus_tag="VM692_17185"
     CDS             2429..3046
                     /locus_tag="VM692_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUQ53966.1"
                     /translation="MTTKRLVALLLLLVPFVGQAQDRMPPIPAGALTEEQALAVAEFA
                     AARGQVTGPWIALLRSPELMKRTRGLSDYLRFESPVPGWLREFVILITARQWGQNYEW
                     NAHYAIAMDEGLSPDIARAIAEGRRPQGMAAEEQILYELVLELQRNHSVSDATYERAV
                     ERFGEQGVVEVVSLAGYYTMISMILNTSRAPLPDGATPALAPFPY"
     gene            complement(3051..>3732)
                     /locus_tag="VM692_17190"
     CDS             complement(3051..>3732)
                     /locus_tag="VM692_17190"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUQ53967.1"
                     /translation="TGSMADPLEHVAEDFTRATGHTLRFESATTGGLLAKIRAGERAD
                     VVVIAAEAAATLEQDGTLAAGTRTPVASSVFGVIVRAGSARPDVTTRDAFRTTVLRAR
                     TISYPDPVVATVSGGYIETVLTELGIKDAARAKASLTPMGYLVGEAVASGEAELGLSF
                     MSEFVGNPALQAIPFPDALQKPQLYSAGVFASSASAAAARELIAYITSAAARAKLAAA
                     GVVPAAAN"
BASE COUNT          571 a         1261 c         1307 g          593 t
ORIGIN      
        1 acttcgcgca gagcgcagcg ctgctcgcgg cgagcgtgac cgcgtggccg gcatggcctg
       61 cgcgcgctgc gttggccgcg gtcgcgccgc tcgacgacgc cacgatcgcg ctcgtcgacc
      121 gcgacctgcc cggcggcgcc gcgttcgcac gtgccgcacg ccagcgcggc atgaccaccc
      181 tcgagttcgc ggccgacgtc gcggagctct ggatgcgcga gctcgagccg cgcttgcgca
      241 acgggcccat caggatcgcg ggccatgcga gcgcggcgac gttgttttgc ctcgacttgc
      301 tcgcgcgcga cttcggtgcg cgtacggtcc ggcgcgtcga cgccgcaacg gccgtgagct
      361 tcgtcatctc gcaatactcg ggaccccgcg ccgcgctcgc gccggctgcc gtgcgcgccc
      421 aatggagaca ttcgtatggc tagtccgccg ccgggcctgt ccgaacgaga tttcgcagcc
      481 gcgctgcgcg acctcgagag cgtcgtcggg cgcgaatggt tgttctcgag cgatgaggac
      541 gtgctgttgt accgcgacgg ttattcgccg ctgtggggcg aggccgagga gcgcaaggcg
      601 tcggcggccg tcgcgccgag ctccgtcgag caagttcagg gcgtcgtaaa ggtcgcgaac
      661 caatacggct tgccgctcta tccgatctcg accgggcgca atctaaccta tggcggcgcg
      721 gcgcccgcat actcgggcag cgtggtcgtc gacttgaagc gcatgaatcg catcctcgac
      781 gtcagcgcgc gcgacaagac gtgcctcgtc gagcccggcg tcagttattt cgatctttac
      841 cggcatctgc gcacgaacaa gatcccgctg tggatcgaca cggccgaccc cggctggggc
      901 gggctcatcg gcaacgcgct cgatcgcggc ggcggctaca ccgacatcga gttccgcgac
      961 cacttcgacg cgcactgcgg catggagatc gtgctgccga cgggcgagct cttgcgcacg
     1021 ggcatgaatg cgagcccgca agccaagacg ggacagctgt tcaaatacgg cttgggcccg
     1081 tgggtcgacg gcatcttctc gcagtcgaac ttcggcatcg tcacgaagat ggggttctgg
     1141 ctcatgcagg agcccgaggc cgcgctgcaa gtctcggtca cggtgccgaa gcgccgcgac
     1201 gtcgtgccgc tcgtcgacac gctgcacagc ctgatgtgtg cgcacgtcgt cccgtcgcag
     1261 gtcaacgtcg tgagtcccgt gatggcgggc aacaacgcgg agatgctgcg gttgcgcggc
     1321 tcgggcggcg cgtcggacga gcagtgggac cgctacgctg caaccgccgg cggcgcgccg
     1381 ttctgggccg cgacgttcgt ctactacggc gcgcgtaacg tgatcgcggc gcagtggcag
     1441 cacacgaagg accgtctcgg cggcattgcg ggtgtcgggt tccaggagac ggcgtcatac
     1501 acgttcccgt tgagcgacga gcaagtcgaa gccgtcgccg acaaggcgcg ctacgggatc
     1561 ccgtcgctga acttgttcgg ctcgcgcacg gcccccggca cgccgccgct cgaaggccac
     1621 atggacttct cgccgatgat cccgccgcac ggcgaggagc tgatcgcgct gacggactac
     1681 acgggcaagg tctacgccga tcaagatttc acgccgtcgt tcgtcggcgg gctcatgttc
     1741 catccgcgca cgatgatctg cttccacgcc gtgcccacgt tccgcaacgc cgcggacaac
     1801 gcgcggacgc gcaagctgtt cgagacgctc gtgcgcggct gcgccgagcg cggttggccg
     1861 atctaccgca tccatcccgt ctttcaaaag ctcggcatgg agatgtttgg gttcaatagc
     1921 ggcgcgctgc atcggttgca cgagacgctg aaggacgcga tcgacccgaa cggcatcgtg
     1981 tcgccggggc gttacgacat ctggccgaaa cggctgcgag gctcgcgcgc atgaaacaca
     2041 cgcagctcgt cgcggtcgcg ctgctcggcg tcgcggcaac cgcggccgca caggacccgc
     2101 aggggccggt cccgatcgcg gcttggagcc gcacgacggc ggccgtcgag acgcaaggcg
     2161 cgcccgtcga gcgcggcgcc gccgtgttca acaactggtg cagtgcctgc cactcgcgcg
     2221 gcccgcagaa cgcgcccggc acgtcctcgc tgcaaatgaa gtatcagggc agcgtgccgg
     2281 ccgcgctcga ggatcgccgc gacctgacac ccgaggtcgt gaagacgttc gtgcgtaatg
     2341 gcgtcgccat gatggcgccg tttcgcaaag tcgaggtcag cgacgccgat ctcgacgcgc
     2401 tcgcggcgta tctgacgcgg tcgcgtccat gacgacgaag cggctcgtgg cgctactgct
     2461 gctcttggtc ccgttcgttg gccaggctca ggatcgaatg ccgccgatcc cggcgggcgc
     2521 gctgaccgag gagcaggcgt tggcggtcgc ggagttcgcg gccgcgcgcg gtcaagtgac
     2581 gggaccgtgg attgcgctgc tgcgcagccc cgagctcatg aagcgcacgc gcgggctcag
     2641 cgattacttg cggttcgaga gcccggtgcc ggggtggttg cgcgagttcg tgatcctcat
     2701 cacggcgcgg cagtggggcc agaactacga atggaacgct cactatgcga tcgcgatgga
     2761 cgagggcctc tcgcccgaca tcgcgcgcgc catcgccgaa ggccggcgac cgcaaggcat
     2821 ggcggccgag gagcagatcc tctacgagct cgtactcgag ctgcagcgca accacagcgt
     2881 cagcgacgca acgtacgagc gcgccgtcga gcggttcggc gagcagggcg tcgtcgaggt
     2941 cgtgagcctc gcgggctact acacgatgat ctcgatgatc ttgaacacgt cgcgcgcgcc
     3001 gctgccggac ggtgccactc cggcgctcgc gccgttcccg tattgatcga tcaattcgcc
     3061 gcggccggca cgacgccggc cgccgcgagc ttcgcgcgtg cagccgcgct cgtgatgtag
     3121 gcgatcagct cgcgcgcggc cgcggcgctc gcactgctcg cgaacacgcc ggcagagtag
     3181 agctgcggtt tctgcagcgc gtcgggaaac ggtatcgctt gcagtgccgg gttgccgacg
     3241 aactcgctca tgaacgacaa gccgagctcg gcttcgccgc tggcgacggc ttcgccgacg
     3301 agatagccca tcggcgtcag cgacgccttg gcgcgcgccg cgtccttgat gccgagctcc
     3361 gtcagcaccg tctcgatgta gccgccggag acggtcgcca cgacggggtc gggatacgag
     3421 atcgtgcgcg cgcgcaggac ggtggtgcgg aacgcgtcgc gtgtcgtgac gtcgggcctg
     3481 gcgctgccgg cgcgcacgat gacgccgaac accgagctcg caaccggtgt gcgtgtgccg
     3541 gcggcgagcg taccgtcttg ctcgagagtc gcggcggctt cagcggcgat caccacgacg
     3601 tcggcgcgct cgccggcgcg aattttcgcg agcaggccgc cggtggtggc gctttcgaag
     3661 cgcagcgtgt ggcccgtggc ccgcgtgaag tcctcggcga cgtgctcgag tggatccgcc
     3721 atcgagcccg tc
//
LOCUS       DATLEE010000784         4309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8153661, whole genome shotgun sequence.
ACCESSION   DATLEE010000784 DATLEE010000000
VERSION     DATLEE010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8153661"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1991)
                     /locus_tag="VM692_17195"
     CDS             complement(<1..1991)
                     /locus_tag="VM692_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HUQ53968.1"
                     /translation="MENPRKLWIALGVLLTLSFGVLLFMGSEIYRQAPPVPERVVAES
                     GELLYRGEDVRQGRRVWQSIGGMQLGSIWGHGSYVAPDWSADWLHREAMFVLDGWATR
                     ELGATSYATLDAEQQAALRGRLETRLRGNTYDAATGTLTLSDDRAAAVEAVTAHYDAL
                     FGSDAALAGLRENYAMRDNTVPDAANRRALAAFVAWTAWATATERPNSKITYTNNWPS
                     EPLIGNRPPATTFLWSAFSVLFLIAGIALLGWHHAVTHSEDEPPALPATDPLAMLRVT
                     PSMHATAKYFWLVLVLFLVQILLGSITAHYQIEGQQLYGYELANVLPYSITRTWHTQL
                     AVLWIATAWLGTGLYIGPAVAGREPRYQRLGVNLLFVCLLVIVAGAFTGQWLAVMQKL
                     GLERNFWFGHQGFEYADIGRFWQVFLFIGLMLWLVLVGRALWPALREGAAENRSIVGL
                     LFLSTVAIGLFYGAGLMWNEATHLSLVEYWRWWLVHLWVEGFFEVFATAVIAFLFTRL
                     GLLRPRVATVAVLFATIVFMAGGVLGTLHHLYFAGTPTAVLALGASFSALEVVPLAYL
                     GFEAYRTYRLGHATPWMARYRWPIRFFVAVAFWNLVGAGLFGFLINPPLSLYYMQGLN
                     LTPLHGHTALFGVYGMLGIGLVLFCLRGLKPHIVWNERLL"
     gene            2110..2760
                     /locus_tag="VM692_17200"
     CDS             2110..2760
                     /locus_tag="VM692_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HUQ53969.1"
                     /translation="MRIDLVSDVACPWCAIGMSALERALTRIGSDIGPVEVHLQPFEL
                     NPTMPVEGADAAEYLSSKYGLSPEQLAVNRARIAERGKAEGFEFGTRAHVWNTFDAHR
                     LLFFAGVEGPPESQRALKRALLAAYHGEGRNISAPDVLLELAVGAGLPQQRTREVLES
                     GAFADEVRQAERFWQEVGIHSVPAVIVDRKHLISGGQPSAVFEQALRQIAAAPSAG"
     gene            2850..3353
                     /locus_tag="VM692_17205"
     CDS             2850..3353
                     /locus_tag="VM692_17205"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUQ53970.1"
                     /translation="MKILRSALLAFGVLAVAATASAAEPEYATIKMQIDIAKPAKEVW
                     SKVGGYCDISKWLNNVDCKITSGDGGIGTVRVLAGGRVTEILVAQTELSYGYTQPVRE
                     GQFYNLYHGFMEARPASGTTTKMLYTLMYDVSNLADKAAKDADIARRRTMFETALMNM
                     KALAEGK"
     gene            3357..>4309
                     /locus_tag="VM692_17210"
     CDS             3357..>4309
                     /locus_tag="VM692_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53971.1"
                     /translation="MTPLHRQSGAAALLATAIFAVAVHGPVLAVDDLAERYVKLVLAV
                     GVHDADYVDSYYGPPDWREAVTAARRGIPELEADAKALLAAIESQPPPAGAEELVQLR
                     HQYLRRQVESLQSRLAMLRGERLAFDVESKLLYDAVAPTHAEQEFDAVLAELARHLPG
                     EGALLDRYQAFRARFEIPKERLSAVFDAAIEGCRARTLKHIDLPAGESFTIEYVTDKS
                     WSGYNWYQGNYRSLIQINTDLPIYIDRAIDLACHEGYPGHHVYNVLLEQQLMRARGWQ
                     EYSVYALFSPQSLIAEGTANYGIEVAFPDDERRAFERDVLFP"
BASE COUNT          790 a         1414 c         1451 g          654 t
ORIGIN      
        1 agcagccgtt cgttccagac gatgtgcggc ttcagcccgc gtaagcaaaa cagcacgagg
       61 ccgatgccga gcatgccgta gacgccgaac agcgccgtat ggccgtgcag cggcgtcaag
      121 ttcaggcctt gcatgtagta cagcgacagc ggcgggttga tcagaaaacc gaagagcccg
      181 gcgccgacga gattccagaa ggcgaccgcg acgaagaacc ggatcggcca gcgatagcgc
      241 gccatccacg gcgtcgcgtg gccgagccgg taggtgcgat acgcctcgaa gccgagatag
      301 gcgagcggca cgacctcgag cgccgagaag ctcgcgccga gggccagcac ggccgtcggc
      361 gtgcccgcga agtagagatg atgcagcgtg ccgagaacgc cgccggccat gaacacgatc
      421 gtcgcgaaca gcacggcaac ggtcgcgact cgcggccgca gcaaaccgag ccgcgtgaac
      481 aggaacgcga tgacggccgt cgcgaagact tcgaagaacc cctcgaccca caggtgcacg
      541 agccaccagc gccagtactc gacgagcgac aagtgcgtgg cttcgttcca catgagcccc
      601 gccccgtaga acaacccgat cgcgaccgtc gacaggaaca agaggccgac gatcgagcga
      661 ttctcggcgg cgccttcgcg cagcgcgggc cagagcgcgc ggccgacgag cacgagccac
      721 agcatcaggc cgataaacag gaacacttgc cagaagcggc cgatgtcggc gtactcgaag
      781 ccttggtggc cgaaccagaa attgcgctcg agcccgagct tctgcatgac ggcgagccat
      841 tggcccgtga acgcgccggc gacgatgacg agcaagcaga cgaacagcag gttgacgccg
      901 agacgttgat agcgcggctc tcggcccgcg acggccgggc cgatgtagag gccggtaccg
      961 agccatgccg tcgcgatcca aagcacggcg agctgcgtgt gccacgtgcg cgtgatcgag
     1021 tacggcagaa cgttcgcgag ctcgtagccg tacagctgtt gcccttcgat ctgatagtgc
     1081 gccgtgatcg agccgagcaa gatctggacg aggaacagca cgagcacgag ccagaagtac
     1141 ttggctgtcg cgtgcatcga cggcgttacg cgcagcatcg ccagcgggtc cgtggccggc
     1201 aacgccggtg gctcgtcctc actgtgcgtg acggcatggt gccagccgag cagagcaatg
     1261 cccgcgatca agaacaacac gctgaacgcc gaccacagga acgtcgtcgc aggagggcgg
     1321 ttgccgatca acggctcgga cggccagttg ttcgtatacg tgatcttcga gttggggcgt
     1381 tccgtcgcgg tggcccatgc cgtccacgcg acgaacgccg cgagcgcgcg ccggttcgcg
     1441 gcgtcgggaa cggtgttgtc ccgcatcgcg tagttttcgc ggagccccgc gagcgcggcg
     1501 tcgctgccga acagcgcgtc gtagtgcgcg gtcacggcct ccacggccgc ggcccgatcg
     1561 tcgctcagcg tcaacgtgcc cgtcgccgcg tcgtacgtgt tgccgcgcaa gcgcgtctcg
     1621 agccgcccgc gcaatgccgc ttgttgctcg gcgtcgagag tcgcgtagct cgtcgcgccg
     1681 agctcccgcg tggcccagcc gtcgagcacg aacatggcct cgcggtgcag ccagtcggcg
     1741 ctccagtccg gcgcgacgta gctgccgtgg ccccagatcg aaccgagctg catgccgccg
     1801 atcgattgcc agacgcggcg gccctggcgc acgtcttcgc cgcgatacag cagctcgcca
     1861 ctctccgcaa cgacgcgctc ggggacgggc ggcgcctgcc gatagatctc gctgcccatg
     1921 aacagcagca cgccgaacga cagcgtcagc agcacgccga gcgcaatcca gagcttgcga
     1981 ggattttcca tgcctcacct cccgacgccg acgttagcgc cgagtgtgca agccgcgtat
     2041 tggggattgc cgcaatctgc ttggcgggtc tcgactatca tggaggcctg accccgacga
     2101 gccgccagca tgagaatcga tcttgtttcc gacgtcgcgt gcccctggtg cgcgatcggc
     2161 atgagcgcct tggagcgcgc gttgacgcgc atcgggtccg acatcggccc ggtcgaggtg
     2221 catctgcaac cgttcgagct aaatcccacg atgccggtcg aaggcgccga cgccgcggag
     2281 tacttgagct cgaagtacgg cttgagcccc gagcagctcg cggtcaatcg cgcgcgtatc
     2341 gcggagcggg gcaaggccga aggcttcgag ttcggcacgc gcgcgcacgt ttggaacacg
     2401 ttcgacgcgc accggctgct gttcttcgcc ggcgtcgaag gcccgcctga gtcccaacgc
     2461 gcgctgaagc gcgcgctgct cgcggcatat cacggcgagg gccgcaacat cagcgcgccc
     2521 gacgtgctgc tcgagctcgc ggtcggcgca ggcctgccgc agcaacgcac gcgcgaagtg
     2581 ctcgagagcg gggcgttcgc cgacgaggtt cgccaggcgg agcgcttctg gcaagaagtc
     2641 ggcatccatt ccgtgccggc cgtcatcgtc gaccgcaagc acctgatctc gggcggccag
     2701 ccgagcgccg tgttcgagca ggcgctgcgg cagatcgcgg ccgcccccag cgcgggctaa
     2761 gcaagagccg ttgcaaacgc gcgggtttat gaacaccatc ttgcgacgcg gacgcgcttc
     2821 ggcgtcgcgc ctcgaataag gagaactaca tgaagatcct gcgttccgct ttgcttgcat
     2881 tcggcgtgct cgccgtggcc gccaccgcat cggccgccga gcccgagtac gcgacgatca
     2941 agatgcagat cgacatcgcg aagccggcga aagaagtctg gtccaaggtc ggcggctatt
     3001 gcgacatctc gaagtggctc aacaacgtcg actgcaagat cacctcgggt gacggcggca
     3061 tcggcaccgt ccgcgtgctg gccggcggcc gcgtcactga aattctcgtc gcgcagacgg
     3121 agctgtcgta cggctacacg cagccggtgc gcgagggcca gttctacaat ctgtatcacg
     3181 gcttcatgga agcgcggcct gcttcgggta cgacgacgaa gatgctgtac acgttgatgt
     3241 acgacgtttc gaatctggcc gacaaagcgg cgaaggacgc cgacatcgcg cgccggcgca
     3301 cgatgttcga gacggcgctc atgaacatga aggcgcttgc agaagggaag tagctcatga
     3361 cgccgctgca ccgccaatcc ggggcggcgg cgttgctcgc cacggcgatt ttcgccgtgg
     3421 cggtgcacgg accggtgctg gcagtggatg acttggccga gcgctacgtg aagctcgtgc
     3481 tcgccgtcgg cgtgcacgat gccgactacg tcgactcgta ctacgggccg cccgactggc
     3541 gcgaggccgt gacggcggcg cgccgcggca tcccggagct cgaagccgac gcgaaggcgc
     3601 tgctcgcggc gatcgagtcg cagccgccgc cggcgggcgc cgaagagctc gtgcagctgc
     3661 ggcatcagta tttgcggcgg caggtcgagt cgctgcagtc gcggctcgcg atgctgcgcg
     3721 gcgagcggct cgcgttcgac gtcgagtcga agctgctcta cgacgcggtt gcgccgacgc
     3781 acgccgagca agagttcgac gcggtgctcg ccgagctcgc gcgacacctg cccggtgaag
     3841 gggcgctgct cgatcgctat caggcgtttc gcgcgcgctt cgagattccg aaggagcggc
     3901 tctcggccgt gttcgacgcg gcgatcgagg gttgccgcgc gcgcacgctg aagcacatcg
     3961 acttaccggc gggcgagagc ttcacgatcg agtacgtcac ggacaagagc tggagcggct
     4021 acaactggta tcagggcaat taccgcagct tgattcagat caacaccgat ctgccgatct
     4081 acatcgaccg cgcgatcgat ctcgcatgcc acgaaggcta ccccggccat catgtctaca
     4141 acgtgttgct cgagcagcag ttgatgcgcg cgcgcggctg gcaggagtac tcggtctacg
     4201 cgctgttctc gccgcaatcg ttgatcgccg aaggcacggc caactacgga atcgaagtcg
     4261 cgtttcccga cgacgagcgg cgtgccttcg agcgcgacgt gctgtttcc
//
LOCUS       DATLEE010000785         3995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6581160, whole genome shotgun sequence.
ACCESSION   DATLEE010000785 DATLEE010000000
VERSION     DATLEE010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6581160"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VM692_17215"
     CDS             <1..1078
                     /locus_tag="VM692_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUQ53972.1"
                     /translation="FIGKNLVARLRETGRDVAALTRQTTAEQLSDELGNADFVFHLAG
                     VNRPQSDAEFNVGNVDYTRALCAALASKQNTAPLVYASSIQATAENAYGRSKRAAEDV
                     VFEHAKSVESRAYVMRLPNVFGKWCRPNYNSAVATFCHQLARGLPIKINDPNAALRLV
                     YIDDLVERLLALLTASLPSGFVEVAPIYETTVGEVVAILQSFEDGRKTLVTPRVGTGL
                     VRALYSTYVSYLPPERFTYDVPRHSDPRGVFVEMLKTPDCGQFSYFTAGPGITRGEHY
                     HHTKTERFLVIHGTARFQFRHILTGEAHEVTLRGGEGRIVESIPGWTHNVTNVGDDEL
                     IVMLWASEIFDRARPDTVPMKVAL"
     gene            1075..2208
                     /gene="wecB"
                     /locus_tag="VM692_17220"
     CDS             1075..2208
                     /gene="wecB"
                     /locus_tag="VM692_17220"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HUQ53973.1"
                     /translation="MKRLKVATVVGTRPEIIRLSRVIARLDEHCEHVLIHTGQNHDYE
                     LNEVFFRDLEVRKPDAFLNTAGTGAAQTIGNVIVAVDDVLSSVRPDALLVLGDTNSCL
                     AVIPAKRRKIPIFHMEAGNRCFDQRVPEEINRRIVDHTADVNMPYSSIAREYLLREGL
                     PPDLIIKTGSPMFEVLTHYRPRIDASDVLTRLNLVTSGYFVVSCHREENIEEKRAFDK
                     LVRVLNMLAEEHDVPVIVSTHPRTRKKIESTGSSFHAHVRLMKPLGFHDYVKLQLMSR
                     AVLSDSGTISEESSILDFPALNIREAHERPEGMEEAAVMMVGLDVARVQQGLAILKKQ
                     RQASARSTRLVADYATPDVSDKVVRVIHSYTDYVNRVVWRKYD"
     gene            2190..3473
                     /locus_tag="VM692_17225"
     CDS             2190..3473
                     /locus_tag="VM692_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUQ53974.1"
                     /translation="MAEVRLSSPAVRFLFVTQVFWPENFRVNDLAAELVARGHEVTVL
                     TGVPNYPEGAVFPAFRNSPETFSRYGGAEIVRIPIIVRGTSRVQLMLNYLSYAVSAFV
                     VGAWKLKGRSFDIIFIYEPSPVTVGLPAVLLRKAKRAAVILWVLDLWPDTLRAIGVVR
                     SPWLLAAVGKLVSFIYDRCDLILGQSPGFISKIRAYCSGPKRIEYFPSWAEALFAEAA
                     ASALPIDIPEAPDKLSLMFAGNIGEAQDFPTILDAAEILRAEGKVRWLIVGDGRMSRW
                     VATEVARRRLEDSVLLLGRHPVEAMPAFFRRADAMLVSLKDEPIFAMTIPGKLQSYLA
                     AGMPIIAMLNGEGADVVERARAGLTCRAGDAQGLAAAALRMASMSRDERRAFGQNALA
                     ANAAEFDRGRLITQFEQWCSELHARNGAVPARGRP"
     gene            3473..>3995
                     /locus_tag="VM692_17230"
     CDS             3473..>3995
                     /locus_tag="VM692_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HUQ53975.1"
                     /translation="MHHVLVVGAGGHGRAVAEVLRQSANCKVVGFLDDAEQGNVWEWP
                     ILGATTTRLESYLASADSAIVAIGNNRRREELQASLQNAGFNMITAVHPRAVVSATAR
                     IGSGCALMAGAIVGTEAVLEEGVIVNSGAVVDHHCRVEAFGHLGTNASMAGGSVLGRG
                     AWMQAGASLGYQVC"
BASE COUNT          749 a         1193 c         1277 g          776 t
ORIGIN      
        1 cttcatcggc aaaaacctgg ttgcgcgtct cagggagacc ggtcgcgacg ttgcggcgct
       61 gacgcggcag actaccgccg agcagctctc agacgagctc ggcaacgccg acttcgtgtt
      121 tcatctcgcc ggcgtcaacc ggccgcagtc ggatgccgaa ttcaacgtcg gtaacgtcga
      181 ctacacaagg gctctatgcg ctgcgttagc tagcaagcag aacacggccc cgctcgtgta
      241 cgcgtcgtcg atacaagcca cggccgagaa tgcctacggc cgcagtaagc gcgcagccga
      301 ggacgtcgtt ttcgagcacg caaagtccgt ggagtcgcgc gcctacgtca tgcggctgcc
      361 gaacgtcttc ggcaagtggt gcagacccaa ttacaactcg gccgtcgcga cgttctgtca
      421 tcaactggcg cgcggtttgc cgatcaagat caacgatccc aatgctgcgc tgcgcctggt
      481 ttacatcgat gacttggtcg agcgattgct cgccctattg accgcctctt tgccgtccgg
      541 ttttgtcgag gtcgcgccga tctacgagac tacggtcggc gaagttgtcg cgatcctcca
      601 aagcttcgag gacggacgaa agaccttggt cacgccgcgt gtcggcacgg gtcttgtccg
      661 tgctttgtac agtacctacg tcagctacct gcctccggag cgcttcacct acgacgtgcc
      721 ccggcacagc gacccgcggg gcgtcttcgt cgagatgttg aaaacgcccg actgcggcca
      781 gttctcatac ttcaccgccg ggccgggtat tacgcgaggc gagcactacc atcacacgaa
      841 gaccgaacgg tttttggtga ttcacggcac ggcgcgcttc cagtttcggc acatattgac
      901 gggcgaggcg cacgaagtga cgctgcgcgg cggcgagggc cgaatcgtcg aatccatccc
      961 ggggtggact cacaacgtaa cgaacgtggg cgacgatgag ctgatcgtca tgctctgggc
     1021 cagcgagatc ttcgaccgcg cacgaccgga taccgtccca atgaaggtcg cattgtgaag
     1081 agactgaaag tagccaccgt cgtcggcact cgtccggaga tcatccggct atcgcgcgtc
     1141 attgcgcgat tggacgagca ctgtgaacac gtgctgatcc ataccgggca aaaccacgat
     1201 tacgagctca acgaagtctt tttccgggac ttagaagtac gtaagcccga cgcatttttg
     1261 aatactgccg gtacaggtgc cgcgcagacg atcggcaacg tcatcgtggc ggtcgacgac
     1321 gtgctgtcat cggttcgccc cgacgcgttg ctcgtgctcg gcgacacgaa tagttgcctg
     1381 gcggtcattc cggctaagcg gcgcaagatc cccatctttc acatggaagc cgggaatcgc
     1441 tgcttcgacc agcgagtgcc cgaagagatc aatcgccgca tcgtcgatca cacggcagac
     1501 gtcaacatgc cgtatagctc gatcgccagg gagtatttgc ttcgggaagg gcttccgccc
     1561 gacctgatca tcaagaccgg cagcccgatg ttcgaggtgc tgacgcacta tcggccccgc
     1621 atcgacgctt ccgacgtcct cacgcgcttg aacctggtca cgagcggcta tttcgtcgtc
     1681 agctgccatc gcgaggaaaa tatcgaggag aaacgcgcgt tcgacaagct cgtgcgcgtg
     1741 ctgaacatgc ttgcagaaga gcatgacgtg cccgtcatcg tgtcgactca cccgcgcacg
     1801 aggaaaaaaa tcgaaagcac gggttcgtca ttccacgcgc acgtgcggct catgaagccg
     1861 ctgggatttc acgactacgt gaagttgcag ctgatgtctc gcgccgtgct ttccgacagt
     1921 ggaaccatca gcgaggagtc gtcgattctc gatttccccg ccctgaatat tcgggaagcg
     1981 catgagcggc ctgaggggat ggaggaggcg gccgtgatga tggtcggcct cgacgtagct
     2041 cgagtacagc aagggttggc gatacttaag aagcagcggc aggcgagcgc gcgttcgacg
     2101 cgcttggttg ccgactacgc gacgccggac gtctccgaca aagtggttcg cgtcattcac
     2161 agctacacgg actacgtgaa tcgggtcgtt tggcggaagt acgactgagc tcgccggccg
     2221 tgcgtttcct tttcgtcact caggtcttct ggccggaaaa ttttcgcgtc aacgacctcg
     2281 cagccgagct cgtggcgcgc ggccacgagg ttacggtttt gacaggcgtt ccaaactatc
     2341 cggaaggggc ggtgttcccg gcgttcagga acagtccgga aactttctcg cgctacggcg
     2401 gcgccgaaat cgtccgaata ccgatcatcg tgcgcggcac gagtcgagtg cagctcatgc
     2461 tgaattactt gagctatgcc gtcagcgcgt tcgtggtcgg cgcatggaag ctcaagggtc
     2521 gatcgttcga catcatcttc atctacgagc cgtctcccgt gacggtggga ttgccggcgg
     2581 tgctgcttcg gaaagccaag cgtgctgccg taatcctttg ggttctcgat ctttggcccg
     2641 acaccttgcg cgctatcgga gtcgtgcgct cgccgtggct gctggcggca gtcgggaagc
     2701 tggtgtcgtt catctacgat cgctgcgacc tcatcttggg ccagtcaccg gggttcatca
     2761 gcaagattcg cgcgtactgt tccggcccga aacgcatcga gtacttcccg agctgggccg
     2821 aggcgctctt cgcggaggcg gccgcgagcg ccctgccgat cgacattccg gaagctcccg
     2881 acaagctgag cctcatgttc gccggcaaca tcggcgaagc gcaggatttc ccgacgatac
     2941 tcgacgccgc cgagatcctc agagcggaag gtaaggtgcg ctggctgatc gtaggcgacg
     3001 ggcgcatgtc gcgctgggtg gcgaccgagg tcgcgcgccg ccgcctcgaa gacagcgtcc
     3061 tgttgctcgg gcgtcacccg gtcgaagcga tgccggcgtt ctttcgccgc gcggacgcga
     3121 tgctcgtttc gctgaaagac gagccgatct tcgcgatgac gattccgggg aagctgcaat
     3181 cgtatctggc agcgggaatg ccgatcatcg ccatgctcaa cggagagggc gctgacgtcg
     3241 tcgagcgcgc tcgggccggc ctgacgtgcc gcgccggtga cgcgcaaggc ctggccgctg
     3301 ccgcgctgcg catggcgtcg atgtcgcgag acgagaggcg ggcgttcggc cagaacgctc
     3361 tcgcagccaa tgcagcggag ttcgaccgcg gtcgtctgat cacgcagttc gagcagtggt
     3421 gcagtgagtt gcacgctcga aacggcgcgg tgccggcgcg cggacggcca tgatgcatca
     3481 cgtgctggtg gtcggcgccg gagggcatgg gcgggccgtc gccgaggtct tgcgacagtc
     3541 ggcaaactgc aaggtcgtag gctttctcga tgacgccgag caagggaatg tgtgggagtg
     3601 gccaatcctc ggcgccacga ccacaaggct cgagagctat ttagcttcgg ccgacagcgc
     3661 gatcgttgcg attggcaaca atcgccgtcg cgaagagctg caagcgagtc tgcagaacgc
     3721 gggcttcaac atgatcacgg ccgttcatcc gcgagcggtc gtatccgcta cggctcgaat
     3781 tggtagcggc tgtgcgctta tggcgggcgc cattgtggga acggaagcag tgctggaaga
     3841 aggcgtgata gtcaattccg gcgccgtcgt agaccatcat tgccgtgtgg aagccttcgg
     3901 tcacctcggg acgaacgcgt cgatggccgg tgggtccgtg cttggtcgag gggcttggat
     3961 gcaggcagga gcttcgctcg gctatcaggt gtgcg
//
LOCUS       DATLEE010000786         5070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10388048, whole genome shotgun sequence.
ACCESSION   DATLEE010000786 DATLEE010000000
VERSION     DATLEE010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10388048"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VM692_17235"
     CDS             complement(<1..615)
                     /locus_tag="VM692_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HUQ53976.1"
                     /translation="MNATSIGTEERAGTGVPGLDDILGGGLPRNRLYLVDGNPGVGKT
                     TLALRFLLHGAQNGERCLYITLSETQTELDAVARSHGWTLDGIHIIELSAVEKSLAGK
                     AQNTMFQSAEVELTHLGKLVLDEVDRQRPQRVVFDSLSELRLLAQNSLRYRRQILAFK
                     TRLAALNATVLLLDDRSAVGADVQVHSIVHGVISMEIAPLKYGVF"
     gene            939..3098
                     /locus_tag="VM692_17240"
     CDS             939..3098
                     /locus_tag="VM692_17240"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HUQ53977.1"
                     /translation="MVRTATPPVIDGKLDEAVWQTADVITDFHQIRPGDGSTPSEPTE
                     VYLLYDDDAFYIGARMHDSEPQLIVAPTNRHNQGLGPDDRLVVIFDPFNTGRSGYRFE
                     TNPNGVRHDALYTTTNSFQGDWTVIWDTAATIDETGWVAEMAIPFKSLPFDPNTQDWG
                     FNFGRGIRRRGEEMAWVSRNRTYNPSILGVATGLQGMNQGVGLDVVPSLAVGDQKTFV
                     SGSGGDTTDSHTSPSLDVFYRFTPSLNGALTINTDFSATEVDARQVNLTRFNLFFPEK
                     RDFFLADADLFQFGRISGNGSNDSNEATSQPSRENARPFFSRRIGLSASGTPVDIEYG
                     GKLSGRVGRFSLGSLAISQDAFGSAGQPGYVEAQDLFVGRVSANVLEESNVGIVVTDG
                     NPQSNVDNSVVGADFHFLNSRIAGGRALEADAWYQQSETPGLVGDDAAFGIGVNMPNT
                     AGLRGGFAVKEIERNFNPALGFISRADVRDMAFDVGYTKFFTGGKVQRLNAGVDWQRF
                     DLLNGGGRQSDQVVFKLLELETPARDAMRLRYSTNEEIVAVPFNVYVDSSDPARNVTI
                     GVGRYTFDEASIRLATGNQRVLSGNVTYLAGDFYNGERTNVSGQFSWKPSRHFILNLD
                     YDWNDVTLPQGDFVTRLVGLSTQVMFSTNLAWITLMQYDNVSEVLGINTRLHWIPKAG
                     QEGFIVLNHNLQDYDKDASFRSAAADVSVKFKYTFRF"
     gene            complement(3129..4703)
                     /locus_tag="VM692_17245"
     CDS             complement(3129..4703)
                     /locus_tag="VM692_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUQ53978.1"
                     /translation="MRGLNRFIAALLVVSLWPAGAVRGQPANDARQVVSRAADALGGA
                     ERIRALRTLRFRAYGHDTYQDGGSRITTEPTAPEKMTILTAYERAIDLANGRTRVRAK
                     QSRAFVFAAQAMMEGRPIDQSLDGSIAFDLAPGGAARRLSSEVAMRRRMELLANPIVA
                     VRAALDSRSRIDNRRADGPATLVDVATATGERFTLAVATSSGLPLWVRWVGPHENLGD
                     LTYRAEFSGYQPVDGLLVPMSFNTVSDFKDNVQLRLHVDRYVLDGDVGDLAAPPAVRS
                     AAEPSSTYTVDANPIAPGVWLLSGSGGANSVLLEFQDHLSLFEVPTSRAWTAALIEKA
                     RSTVPGKPLTEAIISHHHFDHTGGLRTAIAEGLTIVTQAGNVAWFEELAARHVEAFPD
                     ALSRNPRPLETRAVDDHLRLADSKLTVDVYRVVSNNHMAHGLLAYVPEHRLLIQGDLF
                     DLNWEVYFWGNTYEDNLAYRKLDVERDLPIHGGALPLSDVRAKLAEQTRNAEKLCARV
                     AAANLSMPGCPLAWAD"
     gene            complement(4865..5017)
                     /locus_tag="VM692_17250"
     CDS             complement(4865..5017)
                     /locus_tag="VM692_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53979.1"
                     /translation="MPTKQSGGDNKRADREPKLERREERGSLGSGEEEKPADIQPDDV
                     ENGTPR"
BASE COUNT          913 a         1698 c         1591 g          868 t
ORIGIN      
        1 gaacacgccg tacttcagcg gtgcgatttc catcgagatg acaccgtgca cgatgctgtg
       61 cacttgcaca tccgcaccca cggcgctgcg gtcgtcgagc aacagaaccg ttgcgttgag
      121 cgcggccagg cgcgtcttga acgccagaat ctgccgccga tagcgcagcg aattttgcgc
      181 gagcagacgc aactccgaca gcgaatcgaa gacgacgcgt tggggtcgct gacgatctac
      241 ctcgtccaac accaacttgc cgaggtgcgt gagctcgacc tcggcggatt gaaacatcgt
      301 gttctgcgcc ttgccggcga gcgacttctc caccgcagaa agctcgatga tgtggatgcc
      361 gtccagcgtc caaccgtgtg aacgagccac ggcgtcgagc tccgtttggg tctcggagag
      421 cgtgatgtag aggcagcgct caccgttttg cgcgccgtgc agcaaaaacc gcagcgcgag
      481 cgtcgtcttg ccgacgccgg ggttgccgtc gacgagatag aggcggttgc gcggcaagcc
      541 tccgccgaga atgtcgtcta acccgggcac gcccgtgccg gcgcgctctt cggtccctat
      601 ggacgtcgcg ttcacttgtg cggtcccccg tttccttgcc cggcgcgcaa agcatcgcag
      661 cggtctaacc cgaccgcaag atcacgcccg gaaggcgctc cgagcagatg tcatcgttgc
      721 ctacaggaaa ctttgcgccg cgcccgcagc cgtgccggct agactgcggc tcgcgcgagc
      781 gggcccaggg tcgggcagaa cgggatcact gtttggcaaa gaacaaccgg ggactttcag
      841 ccgccgtcgc actggtctgc tccgcgctcg cgctgctcgc cgcccatgcg gacgctcaga
      901 acggctcctc agccgccgac gcgcgcaaaa ccgttcgcat ggtccgtacc gcgacgccgc
      961 ccgtcatcga cggcaagctc gacgaggcgg tctggcaaac ggccgacgtc atcaccgact
     1021 tccatcagat aagacccggc gacggctcca cgccgtccga gccgaccgag gtctacctcc
     1081 tctacgacga cgacgccttc tatataggcg cgcgcatgca cgacagcgag ccgcaactca
     1141 tcgtcgcgcc gacgaaccgg cacaaccagg gcttgggccc cgacgatcgg ctcgtcgtga
     1201 tcttcgaccc gttcaacacg ggccgaagcg gctatcgctt cgagacgaac cccaacggcg
     1261 tgcgccacga cgcgctctat acgaccacga actcctttca gggcgactgg accgtgattt
     1321 gggacacggc cgcgacgatc gacgagacgg gctgggtcgc ggagatggca atcccgttca
     1381 agtcgctgcc gttcgacccc aatacgcaag actggggctt caacttcggg cgcggcattc
     1441 gccggcgcgg cgaggagatg gcctgggtgt cgcgcaatcg cacctacaat ccgagcatcc
     1501 tgggcgtcgc gacgggcctg cagggcatga atcaaggcgt agggttggac gtcgtgccgt
     1561 cgctcgccgt cggcgaccaa aagacgttcg tgagcggcag cggcggcgac acaaccgact
     1621 cgcacacgag cccgtcgctc gatgtgtttt accgtttcac tccgtcgctg aacggcgcgc
     1681 tgacgatcaa cacggatttt tcggcgaccg aagtcgacgc gcggcaagtc aacctcacgc
     1741 gcttcaattt gttcttcccc gagaagcgcg acttcttctt ggccgacgcc gacctattcc
     1801 agttcggccg catcagcggc aacggctcga atgactcgaa cgaagcgacg tcgcagccga
     1861 gccgcgagaa cgcgcggccg ttcttctcgc gccgcatcgg cctcagcgcg agcggcacgc
     1921 ccgtcgacat cgagtacggc ggcaagctga gcgggcgcgt cgggcgcttc agcttgggct
     1981 cgctcgcgat cagccaggat gcattcggga gcgcaggcca accgggctac gtcgaagcgc
     2041 aggacctgtt cgtcgggcgc gtctcggcca acgtgctcga agaatccaat gtcggcatcg
     2101 tcgtgaccga cggcaatccg cagtcgaacg tcgacaacag cgtcgtcggt gccgacttcc
     2161 atttcctgaa ctcgcgcatc gccggcggcc gggcgctcga ggccgacgcg tggtaccagc
     2221 aatcggagac tccaggcctc gtcggcgacg atgccgcctt cggcatcggc gtgaacatgc
     2281 cgaacacggc cggcttgcgc ggcggcttcg cggtcaagga gatcgagcgc aacttcaacc
     2341 cggctctggg cttcatcagc cgcgccgatg tgcgcgacat ggcgttcgac gtcggctaca
     2401 cgaagttttt cacgggcggc aaggtgcagc gcctgaatgc cggtgtcgat tggcagcggt
     2461 tcgacttgct gaacggcggc ggtcgccaaa gcgaccaagt cgtcttcaag ctcctcgagc
     2521 tcgagacgcc cgcgcgcgac gccatgcgcc tgcgctacag cacgaacgag gagatcgtcg
     2581 ccgtcccgtt caacgtctac gtcgattcct ccgaccccgc acgcaacgtg acgatcggtg
     2641 tcggccgcta tacgttcgac gaggccagca tccggctcgc gacgggtaat cagcgcgtgc
     2701 tgtccggcaa cgtcacgtac ctcgcgggcg acttctacaa cggcgagcga accaacgtca
     2761 gcggccaatt ctcctggaag ccgtcgcggc atttcattct gaatctcgac tacgactgga
     2821 acgacgtcac cttgccgcaa ggcgacttcg tgacgcgact cgtcggcttg agcacgcaag
     2881 tgatgttctc gacgaaccta gcctggatca cgctgatgca gtacgacaac gtgagcgagg
     2941 tgctcggcat caacacgcgc ctgcactgga tcccgaaagc cggccaagaa gggttcatcg
     3001 tcttgaacca caacctccag gactacgaca aggacgcgtc gtttcgctcg gccgcggccg
     3061 acgtcagcgt gaagttcaag tacaccttcc ggttctgagc gaagccgaga gcggaaacgt
     3121 caaccgcatc aatccgccca cgcgagcgga cagccgggca tcgacaagtt ggccgccgcc
     3181 actcgcgcgc aaagtttctc ggcgttgcgc gtctgctccg cgagcttcgc gcgcacgtcg
     3241 gacaacggca acgcgccgcc gtgaatcggc aggtcgcgct ccacgtcgag cttgcgatac
     3301 gcgaggttgt cctcgtacgt gttgccccag aagtagacct cccaattgag atcgaatagg
     3361 tcgccctgga tcagcaaacg gtgctcgggc acgtaagcga gcaagccgtg cgccatgtga
     3421 ttgttcgaga cgacacgata gacgtcgacg gtcagcttcg agtccgcgag gcgcaagtgg
     3481 tcgtcgacgg cgcgtgtctc gagcggccgc ggattgcggc tcaaagcgtc ggggaacgcc
     3541 tcgacgtgcc gcgccgcaag ctcctcgaac cacgcgacgt tgccagcctg cgtcacgatc
     3601 gtgagccctt cggcaatggc cgtgcgcaac ccgcccgtgt gatcgaagtg atgatgcgaa
     3661 atgatcgctt cggtcaacgg cttgccgggc accgtgctgc gcgccttctc gatgagcgcg
     3721 gccgtccacg cgcggctcgt cggcacctcg aacaggctca agtggtcctg gaactcgagc
     3781 aacaccgagt ttgcgccgcc ggatccggat agaagccaca cgcccggcgc gatcgggttc
     3841 gcgtcgaccg tgtacgtcga cgacggctcc gcggccgacc ggacggccgg cggcgcagcg
     3901 aggtcgccga cgtcgccgtc gagcacgtag cgatcgacgt ggagccgcaa ttgcacgttg
     3961 tctttgaagt cggacaccgt gttgaagctc atcggcacca agagaccatc gacgggctga
     4021 tagccgctga actcggcgcg ataggtaaga tcgccgaggt tttcgtgcgg gccgacccag
     4081 cggacccaga gcggcaggcc gctcgacgtg gcgacggcga gcgtgaacct ctcgcccgtt
     4141 gccgtcgcga cgtcgacgag cgtcgccggg ccgtccgcac gacgattgtc gatacgacta
     4201 cgtgagtcga gcgccgcgcg caccgctacg atcggattcg cgagcaactc catgcggcgg
     4261 cgcattgcga cctcactcga gagacgcctg gccgccccgc ctggggcgag atcgaaagca
     4321 atgctgccgt ccaagctctg atcgatgggc cggccttcca tcatcgcctg tgccgcgaag
     4381 acgaacgcgc gcgattgctt cgcgcggacg cgcgtgcggc cgttcgcgag gtcgatggcg
     4441 cgctcgtacg cagtcaggat cgtcatcttt tccggggccg tcggctccgt cgtgattcgg
     4501 ctgccgccgt cctgatacgt atcgtggccg taggcgcgaa atcgcagtgt tcgcaatgct
     4561 cgtatccgct cggcgccgcc gagcgcatcc gcggctcgcg atacgacttg acgcgcgtcg
     4621 ttggcgggtt gcccccgcac ggcgcccgcc ggccataggc tcacaacaag cagcgccgca
     4681 atgaaacggt tcaagcctcg catcgaatct cctattcgct ctacgccgtc gcagcggagt
     4741 gagtcgctac tcgttgatcg tgaaacgacc cgctctcgcg ggatcgcacc gcaggttcga
     4801 agaatactcc cactcgagga acggccgaaa cggatgccga cacgtagctc ttactcgcgc
     4861 gcggttagcg cggcgtgccg ttctcaacgt cgtcgggctg aatgtcggcg ggcttttcct
     4921 cttcgccgct gccgagcgac ccgcgttctt cccggcgctc gagcttgggc tcacggtcgg
     4981 cgcgcttatt gtcgcctccg ctctgtttcg tgggcatcgt tcgtcccctt ccttgcgaac
     5041 caaaacgcgg ctgttccgat gcaagacgtg
//
LOCUS       DATLEE010000787         3485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10402977, whole genome shotgun sequence.
ACCESSION   DATLEE010000787 DATLEE010000000
VERSION     DATLEE010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10402977"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /gene="rimO"
                     /locus_tag="VM692_17255"
     CDS             complement(<1..1106)
                     /gene="rimO"
                     /locus_tag="VM692_17255"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HUQ53980.1"
                     /translation="MPKAATKTHAPKVGFVSLGCPKALVDSERILSQLRAEGYETAAD
                     YAGADVVVVNTCGFLDSARTESLDAIGEALAENGRVIVTGCLGAEANVIRDAHPNVLA
                     ITGPHQYEAVVTAVHEAIPPPHDPFVDLVPAQGVKLTPRHYAYLKISEGCNHHCSFCI
                     IPRLRGRLVSRPAADVLGEAERLVKAGVRELLVISQDTSAYGVDVKYQESQWRGRSVA
                     ARITDLSAALGELGAWVRLHYVYPYPHVDDLIPLMADGKVLPYLDVPFQHASPKILKA
                     MKRPAAEAKTLQRIREWRQAVPELTIRSTFIVGFPGETEQDFQTLLDWLEEAELDRVG
                     CFRYEPVVHAAANALPNAVPEELKEERWHRFMAA"
     gene            1253..1714
                     /locus_tag="VM692_17260"
     CDS             1253..1714
                     /locus_tag="VM692_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HUQ53981.1"
                     /translation="MAQTVTRQELTAKVGTELGVSDWFLIDQARVDEFADVTLDHQFV
                     HVDVEKAKRTPFGGTIAHGFLTLSLLVHLCLPFIPELRDRKLLVNYGFDKIRFAAPVR
                     VGKRIRTRAKLAEVTERKPGNVVMRIDVTVEIEGEDKPALVAEWLSLHVVD"
     gene            1701..3416
                     /locus_tag="VM692_17265"
     CDS             1701..3416
                     /locus_tag="VM692_17265"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HUQ53982.1"
                     /translation="MSLIEALARAIAALVCVAAINGCGRGDDGAGTAAAPAAAARIAP
                     AAVTAAEAINEDDLRRVIAEISSDEYGGRGPGSEGEMKTRAFLANELRTLGFAPAGEN
                     GGYEQAVELVGVTTNAPPTWRFESQSGAVELARGTDFIAVSGVQAPSAALDNAELVFV
                     GYGIVAPEYGWNDFKDVDVRGKVIVMLNNDPDWDPQLFAGFERLYYGRWTYKYESAAA
                     HGAAGAIIVHTTPSAGYGWNVVQSSWSGPQFELPAGDEPRTQLRGWVTEDAARRIVGT
                     HDLDALVEQAKSRDFKPVPLGVSTSIALTSSLTRTPSANVLGVMRGSDPELAAQHVIY
                     SAHYDHLGIGDPNAAGDGIYNGARDNASGVAMLVAIGRAFAALPQKPRRSIMLLFVAA
                     EEQGLIGSQYYAEHPTVAPGLIAANVNFDSANIWGETRDITYLGFGKSSLDAVAQDVA
                     AYQGRTVLGDQFPDRGYYYRSDQFNFAKIGVPAFYLRGGTDFAGRPAGWGAEQLERYE
                     ARDYHQPSDELTPEWSFAGIVQDARFGFHAGLLVANDDALPQWNAGNEFEAARRAALE
                     AAGTR"
BASE COUNT          564 a         1146 c         1196 g          579 t
ORIGIN      
        1 tgggccgcca tgaagcggtg ccaacgttct tctttgagct cctcgggcac ggcgttcggc
       61 agtgcgttgg cggctgcgtg cacgaccggc tcatagcgga agcagccgac gcgatcgagc
      121 tcggcttcct cgagccagtc gagcagcgtt tgaaagtcct gctccgtctc gccggggaag
      181 ccgacgatga acgtggaacg gatcgtcagc tcgggcaccg cttggcgcca ctcgcgaatg
      241 cgttgcagcg tcttggcttc ggccgccggt cgcttcatgg ctttcagtat cttcgggctc
      301 gcgtgctgga acggcacgtc gaggtacggc agcaccttgc cgtcggccat cagcgggatc
      361 aggtcgtcga cgtgcgggta cggataaacg tagtgcaagc gcacccatgc gccgagctcg
      421 ccgagcgccg cagaaagatc cgtgatgcgc gccgcgacgg agcggccgcg ccactgcgac
      481 tcctggtatt tgacgtcgac gccgtacgcg ctcgtgtcct gcgagatcac gagcagctcg
      541 cgcacgccgg cctttacgag ccgctcggct tcgccgagca cgtcggccgc ggggcggctg
      601 acgagacgcc ctcgcaagcg cgggatgatg cagaagctgc aatggtggtt acagccctcg
      661 gagattttca ggtacgcgta gtggcgcggc gtcagcttca cgccttgcgc cggcacgaga
      721 tcgacgaacg gatcgtgcgg cggcggaatc gcttcgtgca ccgccgtgac gacggcctcg
      781 tattgatgcg ggcccgtgat cgcgaggacg ttcgggtgcg cgtcgcggat cacgttcgct
      841 tcggcgccga ggcagcccgt cacgatgacg cggccgttct cggcgagcgc ttcgccgatc
      901 gcgtcgagcg actccgtgcg cgcgctatcg aggaacccgc aggtgttcac gacgacgacg
      961 tcggcgccgg catagtcggc cgccgtctcg taaccctcgg cgcgcagctg gctcaggatg
     1021 cgctcggaat cgacgagcgc cttcgggcag ccgaggctca cgaagccgac tttcggggca
     1081 tgcgttttgg tggcagcttt cggcacttgc gggggtctaa acgctcgcga ccggcgtttt
     1141 gggggcgcta ttgcgccacg cacggcggca aaaggcaaac ggctttaccg cacaaggact
     1201 tccggcccac aatcggttgg aacgggcgcc gtcagcgtcc gcgaggaccc gcatggcgca
     1261 gaccgtgacg cgacaggagc ttacggccaa ggtcggcacc gagctcggag tctccgactg
     1321 gttcttgatc gaccaggcgc gcgtcgacga gttcgccgac gtcacgctcg atcatcagtt
     1381 cgtccatgtc gacgtcgaga aagccaagag gacgccgttc ggagggacca tcgcgcacgg
     1441 ctttctgacg ttgtcgttgc tcgtgcacct ctgtctgccg ttcatccccg agcttcgcga
     1501 tagaaagctg ctcgtgaatt acggcttcga caagattcgc ttcgcggcgc ccgtcagggt
     1561 cggtaagcgc attcgcacgc gagcgaagct cgccgaggtc actgagcgca aacccggcaa
     1621 cgtcgtcatg cgcatcgacg tgacggtcga gatcgagggt gaggacaagc cggcgctcgt
     1681 cgccgaatgg ttgagcctgc atgtcgttga ttgaagccct ggcgcgcgcg attgcggcgc
     1741 tcgtctgcgt ggctgcgatc aatggctgcg gacgcggcga cgacggcgca ggcacggcag
     1801 ccgcgccggc ggcagccgcg cgcatcgcgc cggccgcggt gacggctgcc gaagcgatca
     1861 acgaagacga cctgcggcgc gtcatcgccg agatctcgtc ggacgagtac ggcggccgcg
     1921 ggcccggctc cgagggcgag atgaaaaccc gcgcgttcct cgcgaacgag cttcgcacgc
     1981 tcggcttcgc gccggccggc gagaacggcg gctacgagca agccgtcgag ctcgtcggcg
     2041 tgacgacgaa cgcgccgccg acgtggcggt tcgaaagtca gagcggcgcc gtcgagcttg
     2101 cgcgcggcac ggacttcatc gccgtcagcg gcgtgcaggc gccttcggcg gcgctcgaca
     2161 atgccgagct cgtcttcgtc ggctacggca tcgtcgcgcc ggagtacggc tggaacgact
     2221 tcaaggacgt cgacgttcgc ggcaaggtca ttgtcatgtt gaacaacgat cccgattggg
     2281 atccgcagct gttcgccggc ttcgagcgtc tctactacgg acgttggacg tataagtatg
     2341 agagcgcggc ggcgcacggc gccgcgggcg caatcatcgt gcatacgacg ccgtcggccg
     2401 gttacggctg gaacgtcgtg cagtcgtcgt ggagcgggcc gcagttcgag ctgccggccg
     2461 gcgacgagcc gcggacgcag ctgcgcggtt gggtcaccga ggacgcggcg cggcgcatcg
     2521 tcggcacgca cgatctcgac gcgctcgtcg agcaagccaa gagccgcgac ttcaagcccg
     2581 tgccgctcgg cgtctcgacg tcgatcgcgt tgacgagctc gctgacgcga acgccgtcgg
     2641 ccaatgtgct cggcgtcatg cgcggcagcg atcctgagct cgccgcgcag cacgtcatct
     2701 actcggcgca ctacgaccat ctcgggatcg gagacccgaa cgccgcgggc gacggcatct
     2761 acaacggcgc gcgcgacaac gcgagcgggg tcgcgatgct cgtggcaatc ggccgcgcgt
     2821 tcgccgcgct gccgcagaaa ccgcgccgct cgatcatgct gctgttcgtc gcggccgagg
     2881 agcaaggctt gatcggctcg cagtactacg ccgagcaccc gaccgtggcg ccggggctca
     2941 tcgccgccaa cgtgaacttc gacagcgcca acatctgggg cgagacgcgc gacatcacct
     3001 acctcggctt cggcaagtcg agcctcgacg ccgtcgcgca ggacgtggcc gcgtaccaag
     3061 gccgcacggt gctcggcgat cagttccccg atcgcggcta ctactatcgc tccgaccaat
     3121 tcaacttcgc caagatcggc gtaccggcgt tctacctgcg cggcggcacc gatttcgcgg
     3181 gccgtccggc cggctggggc gccgagcagc tcgagcgcta cgaggcccgc gactatcacc
     3241 agccgagcga cgagctgaca cccgagtgga gttttgccgg catcgtgcaa gacgcgcgct
     3301 tcggctttca tgcgggcttg ctcgtcgcga acgacgacgc actcccgcag tggaacgcgg
     3361 gcaacgagtt cgaagccgcg cgccgtgcag cgctcgaagc agcgggtacg cgctgattca
     3421 tgattcgggt tctgctcttc attatcgtcg tgggcgccct cgcgctgatc gtgctgtcgc
     3481 tgttg
//
LOCUS       DATLEE010000788         3177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6592090, whole genome shotgun sequence.
ACCESSION   DATLEE010000788 DATLEE010000000
VERSION     DATLEE010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6592090"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VM692_17270"
     CDS             <1..645
                     /locus_tag="VM692_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HUQ53983.1"
                     /translation="GGSAKQARDREYQAVMPLRGKILNTWEVDPAELLASQEVHNISI
                     ALGVDPGSQNLDGLRYHKICILADADSDGQHIATLLCALFLKHFEPLVAAGHVCVAMP
                     PLFRVDVGKEVHYCLDEAERDLLLDRIEAEKKSGKITVTRFKGLGEMNPSQLRETTMA
                     KETRRLVQLTVEGQTMVDAREVLDKLLAKKRAADRRDWLQDKGNLADLGVDAAG"
     gene            649..2880
                     /gene="parC"
                     /locus_tag="VM692_17275"
     CDS             649..2880
                     /gene="parC"
                     /locus_tag="VM692_17275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HUQ53984.1"
                     /translation="MKDQELNFDGIERVPLSVFTEKAYLDYSMYVILDRALPHLGDGL
                     KPVQRRIIYAMSDLGLSATSKPKKAARTVGDVIGKFHPHGDVACYEAMVHMAQSFSYR
                     YTIVDGQGNWGSQDDPKSFAAMRYTEARLTRYAQVLLNELEQGTVDWQQNFDGTLSEP
                     TLMPAQLPNLLLNGTSGIAVGMSTDVPPHNLKEVASALIKLLDEPDTTLGELMKSIKG
                     PDFPTGGELVSSRADVVQIYKTGTGTLRLRGRYEIEDGEIVITELPFQTSGAKVLEQI
                     AAQMSAKKLPMVEDLRDESDHENPTRLVIAAKSKVDLDGLMNHLFATTELERTVRVNI
                     NVIGLDGRPAVYGLKALLEEWLKFRTTTVKRRLEHRLERVAQRLHILDGLLVAYLNID
                     EVIKIIRREDEPKPVLMKRFKISDAQAEAILELKLRHLARLEEVQIRGEQKALSEERA
                     DLEATLKSKAKLTKLIKTELTELAEKHGDKRRTMIVEREAAQAIDESELVTSEPVTVV
                     LSERGFARAAKGHEIDVIGLSYKAGDRFLAAARGKSNQLAVFIDSTGRSYSVGAHTLP
                     SARGQGEPLAGFFNPPDGASFRAVLIGQPEDKWVVASSAGYGFVVKLEELYSNKKAGK
                     TALRVPEGATVVPATAVGEAGTLLAAASTDGRLLVFKVAELPELPRGKGNKILAVPSK
                     DGVTLAAICVLGKEQALRIDSGERHMTIKPADLAHYAGERGRRGMALPRGWRTVDRLS
                     AEA"
     gene            complement(2864..>3177)
                     /locus_tag="VM692_17280"
     CDS             complement(2864..>3177)
                     /locus_tag="VM692_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUQ53985.1"
                     /translation="RFAIENFGVGRDSGRILAQTSMDFLKIDGSLMQSIATDQALQER
                     VRVYVHAASNRAISTIAERVEDANTMAVLFQLGVAYMQGHYVHEPEVVLADAPAATPR
                     R"
BASE COUNT          602 a         1014 c         1069 g          492 t
ORIGIN      
        1 ggaggctcgg cgaagcaggc gcgcgatcgc gagtaccaag ccgtcatgcc gctgcgcggc
       61 aagatcctga acacttggga ggtcgatccc gccgagctgc tcgcctcgca agaggtccac
      121 aacatcagca tcgcgctcgg cgtcgacccc ggctcgcaga atctcgacgg gctgcgctat
      181 cacaagatct gcatcctcgc agacgctgac tccgacgggc agcacatagc gaccctgctc
      241 tgcgcgctgt tcttgaagca tttcgagccg ctcgtcgcgg ccgggcatgt ttgcgtggcg
      301 atgccgccgc tgttccgggt cgacgtcggc aaagaggtcc actactgcct cgacgaggcc
      361 gagcgcgatc tgctgctcga tcgcatcgag gccgagaaga agtccggcaa gatcacggtc
      421 acgcgcttca aggggctcgg cgagatgaac ccgtcgcagc tgcgcgaaac gacgatggcg
      481 aaggaaacgc gccggctcgt gcagctgacc gtcgaaggcc aaaccatggt cgacgcgcgc
      541 gaggtgctcg acaagctgct cgcgaagaag cgcgccgcgg atcggcgcga ttggctacaa
      601 gacaagggca acctcgccga tctcggcgtc gatgccgcag gctagggtat gaaggatcaa
      661 gagctgaact tcgacggcat cgagcgcgta ccgctctccg tcttcaccga gaaagcgtac
      721 ctcgactact cgatgtacgt gatcctcgat cgtgcgctgc cgcacctcgg cgacgggttg
      781 aagcccgtgc agcgccggat catctacgcg atgagcgact tgggcctgtc ggccacgtcg
      841 aagccgaaga aagccgcgcg caccgtcggc gacgtcatcg gcaagttcca tccgcacggc
      901 gacgtcgcgt gctacgaagc catggtccac atggcgcaaa gcttcagcta ccgctacacg
      961 atcgtcgacg gtcagggtaa ctggggctca caggacgatc cgaagtcgtt cgcggctatg
     1021 cgctataccg aggcgcggct gacgcgttac gcgcaggtgc tgctgaacga gctcgagcag
     1081 ggcaccgtcg attggcagca gaacttcgac ggcacgctca gcgagccgac gctcatgccc
     1141 gcgcagctgc cgaatctgct gctaaacggc acgagcggca tcgctgtcgg catgtcgacc
     1201 gacgtgccgc cgcacaacct caaggaggtt gcgagcgcgc tgatcaagtt gctcgacgag
     1261 cccgacacga cgctcggcga gctcatgaag agcatcaagg ggccggactt ccctacgggc
     1321 ggcgagctcg tctcgagccg cgccgacgtc gtccagatct acaagaccgg caccgggacg
     1381 ctgcgcttgc gcgggcgtta cgagatcgag gacggcgaga tcgtcatcac ggagctgccg
     1441 tttcagacgt ccggcgccaa ggtgctcgag cagatcgcgg cgcagatgtc ggcgaaaaag
     1501 ctgccgatgg tcgaagacct tcgcgacgag tccgaccatg aaaacccgac gcggctcgtc
     1561 atcgccgcga agtcgaaggt cgacctcgac ggcttgatga accacctgtt cgcgaccacg
     1621 gagctcgagc gcacggttcg cgtcaacatc aacgtgatcg gcctcgacgg ccggcccgcc
     1681 gtctacggct tgaaggcact gctcgaggag tggctgaagt tccgcacgac gacggtcaag
     1741 cgccggctcg agcaccggct cgaacgtgtc gcgcagcggc tgcatatctt ggacgggctg
     1801 ctcgtcgcgt acctcaacat cgacgaggtc atcaagatca tccgccgcga ggacgagccg
     1861 aagcccgttc tcatgaagcg cttcaagatc tccgacgcgc aggccgaggc gatcctcgag
     1921 ctgaagttgc gccacctcgc gaggctcgaa gaggtgcaga tccgcggcga gcagaaggcg
     1981 ctcagtgaag agcgcgccga cctcgaagcg acgctgaagt cgaaggcgaa gctgacgaag
     2041 ctgatcaaga cggagctcac ggagcttgcc gagaagcacg gcgacaagcg ccgcacgatg
     2101 atcgtcgagc gcgaggccgc gcaggccatc gacgagtcgg agctcgtgac gagcgagccc
     2161 gtgaccgtcg tgctttccga gcgcggcttc gcacgtgcgg ccaaaggcca tgagatcgac
     2221 gtgatcggcc tgtcgtacaa ggccggcgat cgattcttgg cggccgcgcg cggcaagagc
     2281 aatcagctcg cggtgttcat cgacagcacg ggccgttcgt acagcgtcgg cgcccacacg
     2341 ttgccgtcgg cgcgcggcca gggcgagccg ctcgcgggct tcttcaatcc gccggacggc
     2401 gcgagcttcc gcgcggtgct catcggccag cccgaggaca agtgggtcgt cgcgtcatcg
     2461 gccggctacg gcttcgtcgt caagctcgag gagctctact cgaacaagaa ggccggcaag
     2521 acggcgctgc gcgtgcccga aggggcgacg gtcgtcccgg caactgccgt cggcgaggct
     2581 gggacgttgc tcgcggccgc gtcgacggac ggccggctgc tcgtgttcaa agttgctgag
     2641 ctgcctgagc tgccgcgggg caaggggaac aagatcctcg cggtgccgag taaggacggt
     2701 gtcacgctcg cggcgatttg cgtgctcggc aaggagcaag cgctgcgcat cgattccggc
     2761 gagcggcaca tgacgatcaa gccggccgac ctcgcgcact atgcgggcga gcgcggccgg
     2821 cgcggcatgg cgttgccgcg tggctggcga accgtggatc ggctcagcgc cgaggcgtag
     2881 cggccggagc gtccgcgagc acgacctcgg gctcgtggac gtagtggcct tgcatgtagg
     2941 cgacaccgag ctgaaacagg acggccatcg tattggcgtc ctcgacgcgc tcggcgatcg
     3001 tcgaaatggc tcgattcgaa gcggcgtgca cgtagacgcg taccctttcc tgcagcgcct
     3061 ggtccgtcgc gatgctttgc atcagcgagc cgtcgatttt caggaagtcc atcgacgttt
     3121 gggcaaggat gcgacccgag tcgcggccga cgccgaagtt ttcgatcgca aaccggt
//
LOCUS       DATLEE010000789         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10411859, whole genome shotgun sequence.
ACCESSION   DATLEE010000789 DATLEE010000000
VERSION     DATLEE010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10411859"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VM692_17285"
     CDS             <1..1177
                     /locus_tag="VM692_17285"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HUQ53986.1"
                     /translation="LVAVAAAALASVARAHEVPSDVTVRMLVRPEGERLKLLVRAPLE
                     AMQDITFPLVGPGYLDVPRAESQLRDAARLWLADNVDLYEGDRRLPLAIVAVRASIPS
                     DRSFAEYDAALAHLHAAPLPAGLDLPWQQALLDVELEAPIESPTSLFSISARTGRLGM
                     RVVNAVRFFGASGVERVFQIEGNSGLVALDPRWHQSMLRFLVQGFEHILSGADHLLFL
                     LALVVPFRRELRKLVWIVTAFTGGHSVTLIASAYGVAPSALWFPPLVETLIALSIFYM
                     CVENVVCPNPRTRWPLAFAFGLVHGFGFSFALQNTLQFAGDHVLTSLLSFNVGIELGQ
                     LLVLAAVVPALNLLFRFVDGRIGGIVIAVIVGHTAWHWLGQRFDALSAFFGGWHWPG"
     gene            1259..1660
                     /locus_tag="VM692_17290"
     CDS             1259..1660
                     /locus_tag="VM692_17290"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HUQ53987.1"
                     /translation="MSSRTLVCSIASLTLAAATTALAHHSFSAEFDANKRVTMTGTVT
                     KVEWRNPHTWFYMDVKGDNGDVANWGLELASPNLLLRNGWTRSSMKMGDVVTVEAYLA
                     KNGMNLANAHVVTLASTGASLFTGSSKGSTP"
     gene            1657..2466
                     /locus_tag="VM692_17295"
     CDS             1657..2466
                     /locus_tag="VM692_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ53988.1"
                     /translation="MSRVVAIVALVLAAPCAYAQWEKVPQASVPRTRDGAANLTAPAP
                     KGRARKPDLSGVWLADSEPLPQEAGLTIEGDMPFPRYMVNVLADVPFEQAPLQPWAAE
                     LFQKRLQSGGTDDPVAHCKPTGLPLLNTAPVPFKLVQMPDLVVLLYEENSVFRQIFLD
                     GRKPVDDPVPQALGYSTGRWEGRELVVDTVGFSDRHWLDAMGHPLTEQLHLVERFRRP
                     DAGHLEIETTIDDKGAYTKPFTYTVKLTLIPDDDLLEYFCTDNEKDSQHYQ"
BASE COUNT          387 a          846 c          856 g          459 t
ORIGIN      
        1 gctggtggcg gttgcggccg cagcgctggc atccgtcgcg cgggcgcacg aggtgccgag
       61 cgacgtcacg gtgcgcatgc tcgtgcgtcc ggaaggcgag cgcttgaagc tcctcgtgcg
      121 cgcgccgctc gaggcgatgc aggacatcac gttcccgctc gtcggtcccg gctatctcga
      181 cgtgccgcgc gccgagagcc agctgcgcga tgcggcgcgg ctctggctcg ccgacaacgt
      241 cgatctctat gaaggcgacc ggcgtctgcc gctggcgatc gtcgccgtgc gtgcctcgat
      301 tccgagcgac cggtcgtttg ccgagtacga cgcggcgctc gcgcacctgc atgcggcgcc
      361 gctgccggcg ggtctcgacc tgccgtggca gcaggcgttg ctcgacgtcg agctcgaggc
      421 gccgatcgag tcgccgacgt cgctgttctc gatcagtgcg cgcacgggac ggctcggcat
      481 gcgcgtcgtc aacgccgtgc gcttcttcgg cgcgagcggc gtcgagcgtg tgttccagat
      541 cgagggcaac tcggggttgg tagcgctcga tccgcgttgg caccaatcga tgctgcggtt
      601 cctcgtgcag ggcttcgagc acatcttgag cggcgcggat catctactct tcttgctggc
      661 gctcgtcgtg ccgttccggc gggagctgcg caagctcgtc tggatcgtca cggcctttac
      721 gggcgggcac tctgtgacgc tgatcgcctc ggcatatggc gtcgcgccga gcgcgttgtg
      781 gtttccgccg ctcgtcgaga cgctgatcgc gctgtcgatc ttctacatgt gcgtcgagaa
      841 cgtcgtctgc ccgaatccac gcacgcgctg gccgctcgca ttcgccttcg ggctcgtgca
      901 cggcttcggc ttctcgttcg cgctgcagaa cacgttgcag ttcgcgggcg atcacgtgtt
      961 gacgtcgctg ctgtcgttca acgtcggcat cgagctcggc caattgctcg tcctggccgc
     1021 ggtcgtgccg gcgctgaacc tgctgttccg tttcgtcgat gggcgcatcg gcggcatcgt
     1081 gatcgccgtc atcgtcggcc ataccgcgtg gcattggctc gggcagcgct tcgacgcgct
     1141 gagcgcgttc ttcggcggct ggcactggcc gggatgacgg cgagcggcgc ggccctttcc
     1201 aaggccggag cagtgcgcca caatccactc actcttccgc gcacaccggg agcttcgcat
     1261 gagcagcagg accctcgtct gttctatcgc gagcttgacc ctcgccgccg ccacgaccgc
     1321 gctagcgcac cactcgttct ccgccgagtt cgacgccaac aagcgagtca cgatgacggg
     1381 caccgtcacg aaggtcgagt ggcgcaaccc gcacacgtgg ttctacatgg acgtcaaggg
     1441 cgacaacggc gacgttgcga actggggctt ggagctcgcg agcccgaatc tcctgctgcg
     1501 caacggctgg acgcgctcgt cgatgaagat gggcgacgtc gtcacggtcg aggcgtacct
     1561 cgccaagaac ggcatgaacc tggccaacgc gcacgtcgtg acgttggcga gcacgggcgc
     1621 gagcttgttc acgggctcga gcaaaggcag cacgccgtga gccgcgttgt tgcgatcgtg
     1681 gcgctcgtgc tcgcagcgcc gtgcgcgtac gcgcaatggg agaaggtgcc gcaggcgtcc
     1741 gtgccgcgca cgcgcgacgg tgccgcgaac ctgacggcgc cggcgccgaa ggggcgggca
     1801 cgcaaaccgg atttgagcgg tgtttggctc gccgacagcg agccgttgcc gcaagaggcg
     1861 ggcttgacca tcgaaggcga catgccgttt ccgcgctaca tggtcaacgt gctcgccgac
     1921 gtcccgttcg agcaggcgcc gctgcagccg tgggccgcgg agctgtttca gaagcgtttg
     1981 cagagcggcg gcaccgacga tcccgtcgcg cactgcaagc cgaccggcct accgctgctg
     2041 aacacggcgc ccgtcccgtt caagctcgta cagatgccgg accttgtcgt gctgctgtac
     2101 gaggagaaca gcgtgttccg ccagatcttt ctcgacggtc gcaagcccgt cgacgaccca
     2161 gtgccgcaag cgctgggcta ttcgacgggc cgatgggaag ggcgcgagct cgtcgtcgac
     2221 accgtcggct tcagcgatcg acattggctc gacgcgatgg gtcatccgct gacggagcaa
     2281 ctgcacctcg tcgagcgctt tcgtcggccc gatgccggcc acctcgagat cgagaccacg
     2341 atcgacgaca agggtgcgta cacgaagccg ttcacataca ccgtgaagct gacgttgatt
     2401 cccgacgacg atctgctcga gtacttctgc accgacaacg agaaggactc gcagcactac
     2461 cagtgacggc gaatcacggg tggctgttgt acctccctcg cgcggagccg ccaacccctt
     2521 cgcaaaactg aagtcgctgg tgctagcg
//
LOCUS       DATLEE010000790         3170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_8184527, whole genome shotgun sequence.
ACCESSION   DATLEE010000790 DATLEE010000000
VERSION     DATLEE010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8184527"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VM692_17300"
     CDS             <1..330
                     /locus_tag="VM692_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HUQ53989.1"
                     /translation="VSPMSTVPPRKLTSPREPASNGRAPIGWSLIGQQGFPVITVPAG
                     FTNNVWDRVVDGDGTRLVGPVAAALPVGVDFIARPFDEPLLLGIASAYEAATQHRKPP
                     AEFGAVP"
     gene            567..2837
                     /locus_tag="VM692_17305"
     CDS             567..2837
                     /locus_tag="VM692_17305"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUQ53990.1"
                     /translation="MKIGRSLASLLVCVLTACGGGGGGSPGSPPATTPPPATTPPANN
                     AAPSAANDSYSASRDNALAVAAPGVLANDSDAEKDALTVRLDRNAAHGTLALQANGSF
                     SYTPTSGFTGTDEFTYIANDGKADSAAATVKIGVGDTWLFSDSFSRATSPSVGNGWVE
                     LEAAGAAVVLDGARLSFADTSDAVMRPLVRHGFNPVTSGLLEWEFEADWTKTGNDDGY
                     EVHMQLGSGAQMTASSPTAGVGIDLVWGRVGGVDQTLGYRRAGATTALTPVSGKATIR
                     VRVDVAALAYDVFVNGQSVGSKIPVETSVTFDTLRFFTDNLNETAFSGRTFDSIVVKA
                     WHADGVTRNSAPVVPDQVVWADDALAKTITLSYTDGDGPGPYTFDIVDGPSHGTLGSD
                     DGDATVVYTPTAGYLGRDSFTFRVSDGTANSGLASMSVVVQHYPGATWDTKTPAEVGL
                     RQAGLDALAASIGGVGSVVRNGYMVYTWGDQTSKADWASATKPVLHTLLFFAVQEKKL
                     ASLDERIGNWVLPATGGTLRPEDEPMTFTHLMNMTSGYALVEAPGAAFAYNDIASQLK
                     NKLIGAVLGEPLEGQLQSRFAPLQFQDGAMLTVRGGYGLSTTTRDFARIAWFWMNHGS
                     WKNQQLLPSAFFDQYMRAQVPGTTPRSAGPDADYLNVGSVGGGADQSEFGPGLYGTAW
                     WFNETVGTTGLRGWPDAPIDMFQANGHWTQEIVVMVPSLNLVVAARGAWGSFVPGDAN
                     SGMNLRMRQLTQAVVQ"
BASE COUNT          561 a          987 c         1060 g          562 t
ORIGIN      
        1 gtgtcgccga tgtcgacggt gccgccgcgc aagctgacgt cgccgcgcga gcccgcgtcc
       61 aacggacgcg cgccgatcgg ctggtcgctg atcggccagc aaggatttcc cgtgatcaca
      121 gtgccggccg gcttcacgaa caacgtgtgg gatcgcgtcg tcgacggcga cggcacgcgc
      181 ctcgtcggcc ccgtggcagc ggctcttccg gtcggcgttg atttcatcgc gcgcccgttc
      241 gacgaaccgc tgttgctggg catcgcgtcc gcctatgaag cggcgacgca gcaccgcaag
      301 ccgccggccg agttcggagc cgtcccctaa cccgccgtcc gatggctctc gcgactctga
      361 accgtgacac cgttgcagaa ttcgctgcgt aattcgcaag acgagaatgc ttgccactaa
      421 gcggcgcgaa cgttttgatc gcgctcgccc gtatttccgc cggatcagcg ctggttctcg
      481 cccgcgtcga gcagtggcac atcgcttgca tcacacctcc tcgagccgca tgactgcggc
      541 tgacgtcctt ctgggaggag gggtgcatga aaatcgggcg aagtctcgcg tcgttgctcg
      601 tctgtgtact tacggcatgt ggcggaggag ggggcggctc tccgggttcc ccaccagcaa
      661 ccaccccgcc gccggcaacc acgcccccag ccaacaacgc tgcgccgtcc gcggcgaacg
      721 acagctacag cgcaagtcgc gacaacgcgc tggccgttgc ggcgccgggc gtgctggcga
      781 acgacagcga cgccgaaaaa gatgcgctga cggtgcggct cgatcgcaac gcagcacacg
      841 gcacgttggc gctgcaggcc aacggctcct ttagctatac gccgacgtca ggattcaccg
      901 gaacggatga gttcacctat atcgcgaacg atggcaaggc cgacagcgcc gcggccacgg
      961 tgaaaatcgg cgttggcgac acgtggctgt tctcggactc cttcagccgc gcgacgagtc
     1021 cttcggtcgg caatggctgg gtagagctcg aagccgctgg ggccgcggtc gtgctcgatg
     1081 gcgcccgtct cagcttcgcg gatacttcgg acgccgtgat gcggccgctc gtgaggcacg
     1141 gtttcaaccc agtgacttca gggttgctcg agtgggagtt cgaggccgac tggaccaaga
     1201 ccggcaacga cgacggttac gaagtgcaca tgcaactcgg cagcggggcg caaatgaccg
     1261 cgagctcgcc cacggccgga gttggcatcg atttagtgtg gggacgcgtc ggcggggtcg
     1321 atcagacact cggctatcgg cgagccgggg cgacgacggc gctgacgccg gtgtccggga
     1381 aggcgacgat ccgcgtgcgc gtcgatgtcg ccgcgcttgc gtacgatgtc ttcgtcaatg
     1441 gtcagagtgt cggctcgaag atcccggtcg aaacgtccgt cactttcgac acgctgcggt
     1501 tctttaccga taacctcaac gagacggcgt tctctggccg gacgttcgac tcgattgtcg
     1561 tcaaagcgtg gcatgccgac ggagttacgc ggaactcggc tcccgtggtt cccgatcaag
     1621 tcgtctgggc cgatgatgcg ctcgcgaaga cgatcacgct gtcgtacacg gacggcgacg
     1681 gtccgggacc gtacacgttc gacatcgtcg acgggccaag tcacggcacg ctcggcagcg
     1741 acgacggtga tgcgaccgtc gtatatacgc cgactgcggg ctatctcggg cgtgacagct
     1801 tcacgttccg tgtcagcgac ggcaccgcca actccggcct cgcgtcgatg tctgtcgtcg
     1861 tccaacacta tccgggcgcc acgtgggaca cgaaaacgcc cgcggaggtc ggcctgaggc
     1921 aggcggggct cgatgccctc gcggcgagca tcggcggtgt cggcagcgtc gtcagaaacg
     1981 gttacatggt ttacacgtgg ggcgaccaaa cttcgaaagc ggattgggcg tcggcgacta
     2041 agcccgtgtt gcatacgttg ctgttcttcg ccgttcagga gaagaaactt gcgtctctcg
     2101 atgagcgcat cgggaactgg gtgctccccg cgacgggcgg cacgctgcgc cccgaggacg
     2161 agccgatgac gtttacgcat ctgatgaaca tgacgagcgg ctacgcgctc gtcgaagctc
     2221 ccggcgccgc gtttgcctac aacgacattg cgtcgcaact gaagaacaag ctgattggcg
     2281 ccgtactcgg tgagccgctg gaagggcagc tgcagtcgcg cttcgccccg ctacagttcc
     2341 aagacggcgc gatgctcacg gtgcgcggcg ggtacggtct ttcgacgacg acgcgcgatt
     2401 ttgcccgcat cgcatggttc tggatgaacc acggcagctg gaaaaaccag caactgctgc
     2461 cgagcgcgtt cttcgatcag tacatgcgag cccaggtgcc cggtacgacg ccgcggtctg
     2521 cggggccgga cgctgactac ttgaacgtcg gcagcgtcgg cggtggcgca gaccagtcgg
     2581 aattcggtcc ggggttgtac ggcacggcct ggtggttcaa cgagacggtc ggcacgaccg
     2641 gccttcgcgg ttggcccgac gcaccgatcg atatgttcca ggcgaacggt cattggaccc
     2701 aagagatcgt cgtcatggtc ccgagcttga atctcgtcgt agcggctcgc ggcgcttggg
     2761 gaagctttgt gcccggcgac gcgaactccg gtatgaacct tcggatgcga cagttgacgc
     2821 aagccgttgt gcaataggac aaccggttcc ggcgcgatcg tcgtgctgac gcggcgcttg
     2881 ttgacgaagc gattcagccg gcggcggagg aacgaagtgc ttcatacgca gcggacgttg
     2941 taaagcgggc gggcaatcga tgtgcggaat tgccggcata gttccgagtc gcgacttagc
     3001 ccctgcgcag atggagcgtt gggtgcgacg gatgtgccaa tcaatggtcc atcgcggccc
     3061 cgacgatgaa ggggtgtaca tcgcaccgga tatcgggatc ggcgtgcgcc gcctcgcgat
     3121 catcgatatc gagaacgggc gccagccgat gtacagctcg gatgggcgcc
//
LOCUS       DATLEE010000791         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_10420157, whole genome shotgun sequence.
ACCESSION   DATLEE010000791 DATLEE010000000
VERSION     DATLEE010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10420157"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VM692_17310"
     CDS             <1..765
                     /locus_tag="VM692_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUQ53991.1"
                     /translation="GYVVARRQATVSAKVTGKVAAVLIEEGQRVEAGEVIARLDDANA
                     RAQVAQAAAQVEQQRASLNAVHVAFMNAGPTFDRNRRQFAAKFISAQSYDEAKTGYEA
                     AEASLAVAARALAVAEATLAVSQRNLDDTVVRAPFGGIVTVKAAQEGEMVSPVSAGGG
                     FTRTGLGTIVDMDSLEVEVDVSENFISRVETGQGAAITLNAYPDRRIAGRVIAIIPTA
                     DRAKATVKVRVGFEQLHAGVLPEMGARVAFLGPEAQ"
     gene            762..1457
                     /locus_tag="VM692_17315"
     CDS             762..1457
                     /locus_tag="VM692_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUQ53992.1"
                     /translation="MTSIVSIKGVAKAYVRGKQRVDVLRGIDLEVEQGEFLALMGPSG
                     SGKTTLLNLIGGLDRPDRGTITVAGERLEALSQAALTKWRSRHVGFIFQFYNLLPVLT
                     AERNVEVPLLLTKLNAKERHRNVTAALTLVGLADRAKHKPAELSGGQQQRVAIARALV
                     SDPSVLVCDEPTGDLDRDTSDAILALLQLLNRTHGKTIVMVTHDPRAAGHASRRVYVD
                     KGVLGRAPIDDAA"
     gene            1454..>2037
                     /locus_tag="VM692_17320"
     CDS             1454..>2037
                     /locus_tag="VM692_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ53993.1"
                     /translation="MKYLPLVWAGLWRKRGRTVLTMLSVAIAFGLHAMLDGVTAAFDD
                     ALMAMTDAARLRTASRVNVRAGLPLSYRARIEAVPGVRDVGMVTFVGGNYRDATEFIE
                     VSAIDVDRLDVGGSFDVDPEYVEQMHRVRTGAIIGPELVRRYGWKIGERVTLRSPVFS
                     QARSTGRSISSASTRYPMGRSRPTATSGSTTRIST"
BASE COUNT          339 a          684 c          697 g          317 t
ORIGIN      
        1 ggctacgtcg tcgctcgacg ccaagccacg gtgtccgcga aggtgacggg taaggtggcg
       61 gccgtcctga tcgaggaagg tcagcgagtc gaggccggcg aggtcatcgc gcgcctcgac
      121 gacgcgaatg cgcgcgcgca ggtcgcacaa gcggcggcgc aagtcgagca acaacgcgcg
      181 agcttgaacg ccgtgcacgt cgccttcatg aacgcgggcc cgacgttcga tcgcaaccgg
      241 cggcagttcg ccgcgaagtt catcagcgca cagagctacg acgaagcgaa aaccggttac
      301 gaggctgcgg aagccagcct tgcggtcgct gcgcgcgcgc ttgccgtggc cgaggcgact
      361 ctcgccgttt cgcagcgcaa tctcgacgac accgtcgtgc gcgcgccatt cggtgggatc
      421 gtgacggtca aggccgcgca agagggcgag atggtttcac cggtctcggc cggcggcggt
      481 ttcacgcgca caggtctagg cacgatcgtc gacatggact ccctcgaggt cgaggtcgac
      541 gtcagcgaga acttcatcag ccgcgtcgaa acgggccagg gtgccgcgat cacgctgaac
      601 gcgtatccgg accggcgcat cgcgggccgc gtgatcgcga tcatcccgac agccgaccgc
      661 gccaaagcca ccgtcaaggt tcgcgtcggc ttcgagcagc tccacgccgg cgtgctaccc
      721 gagatggggg cccgcgtcgc gtttttgggc ccggaagcgc aatgaccagc atcgtgtcga
      781 tcaaaggcgt cgcgaaggcc tacgtccgcg gcaagcagcg agtcgacgta ttgcgcggca
      841 tcgatctcga ggtcgagcag ggcgagttcc tcgccttgat gggcccatcg ggctcgggca
      901 agacgacgct gctgaatctg atcggcggcc tcgaccgccc cgaccgcggc acgatcacgg
      961 tcgccggcga acggctcgaa gccctgtcgc aggcggcgct cacgaagtgg cgttcgcggc
     1021 acgtcggttt catatttcag ttctacaatt tgttgccagt gctgacggcc gagcgcaacg
     1081 tcgaagtgcc gttgctattg acgaagctca acgccaagga gaggcaccgc aacgtcacgg
     1141 cggcgctgac gctcgtcggg ctcgccgacc gcgccaagca caagccggcc gagctctcgg
     1201 gcggccagca gcagcgcgtc gcgatcgcgc gcgcgctcgt cagcgatcca tcggtgctcg
     1261 tctgcgacga gccgaccggc gacctcgatc gcgacacttc cgacgcgatt ctcgcgctgc
     1321 tgcagctgct gaaccgcacg cacggcaaaa ccatcgtcat ggtgacgcac gacccgcgcg
     1381 ccgcgggcca cgcgtcgcgc cgcgtctacg tcgacaaggg cgtcctcggc cgcgcgccga
     1441 tcgacgacgc tgcgtgaagt acctgccgct ggtctgggcg ggactgtggc gcaagcgcgg
     1501 gcgcacggtc ctgacgatgc tatcggtcgc catcgcgttc ggtctccacg cgatgctcga
     1561 cggcgtaacg gccgccttcg acgacgcgct gatggcgatg acggacgcgg cccgcctgcg
     1621 tacggcgagc cgcgtcaacg tccgcgcggg cttgccgctc tcttaccgcg cgcgcattga
     1681 ggccgtgccc ggcgtgcgcg acgtcggcat ggtgacgttc gtcggcggca actaccgcga
     1741 cgccacggag ttcatcgagg tgtcggcgat cgacgtcgac cggctagacg tgggcgggag
     1801 cttcgacgtc gaccccgagt acgtcgagca gatgcaccgc gtgcgcacgg gcgcgatcat
     1861 cggccccgag ctcgtacgcc gctacggttg gaagatcggc gaacgcgtga cgttgcgctc
     1921 gccggtgttc agccaggctc gctcgactgg acgttcgata tcgtcggcgt ctacgcgata
     1981 cccgatgggg cgttcccggc cgacggcaac ttctggatca actacgcgta tttcgac
//
LOCUS       DATLEE010000792         2329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6620752, whole genome shotgun sequence.
ACCESSION   DATLEE010000792 DATLEE010000000
VERSION     DATLEE010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6620752"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VM692_17325"
     CDS             <1..486
                     /locus_tag="VM692_17325"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106411 - XMP 5'-nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HUQ53994.1"
                     /translation="GINNGANLGDDVLYSGTVAAAMEGRFLGIPSIAVSLVLNGFEHY
                     ATAAKLIRKLVERNITDPLPTDVILNINVPDVPYDQLKGVRATRLGFRHKSEPAVKAL
                     DPHNRPIYWIGPAGAGQDTGPGTDFHAIAQGFVSVSPIKVDLTAHASIEKVATWFERF
                     R"
     gene            483..1151
                     /locus_tag="VM692_17330"
     CDS             483..1151
                     /locus_tag="VM692_17330"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HUQ53995.1"
                     /translation="MNGATRNGIGMTSARTRDRLIERLKAEGITNHGVLERVRAVPRH
                     LFVDEALASRAYEDTALPIGFGQTISQPYIVAAMTEALIESGKKLTRVLEIGTGCGYQ
                     TAVLAPFVQHIYSVERIQPLVQRARGMLAELRITNVSLRHADGWHGWRSQAPFQGMIV
                     AAAPEMVPPELYEQLDEGGRLVIPIGARGRQTLLRVTRNGDEFKRENLLPVSFVPFVE
                     GTET"
     gene            1286..2074
                     /locus_tag="VM692_17335"
     CDS             1286..2074
                     /locus_tag="VM692_17335"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HUQ53996.1"
                     /translation="MACTTASNPPLSRRDARAAVHVVQRGETLSKIAWQHRVDPQELA
                     NWNGVTDPDALRIGQRLRLVPPRGYVALPTPPPVARTAPNAAPPPAPTRATPPRSSSA
                     PRSSTTAPQQRPSAAPPARPSSTPARQSAPPPAQASPPRWSWPTDGRIVAGFGSADGI
                     ESGIGIAGREGQPVRAAAAGRVVYAGGGLMGYGQLVIIKHDETFLSAYGYNSELLVTQ
                     GQDVVRGATIALMGQGPGRQARLHFEIRRNGVPVDPLLFVTAPR"
BASE COUNT          369 a          811 c          800 g          349 t
ORIGIN      
        1 ggcatcaata acggcgcgaa cctaggcgac gacgtgctgt actcgggcac cgtggcggcg
       61 gcgatggagg gacgtttcct cggcattccg tcgatcgccg tgtcgctggt cctgaacggc
      121 ttcgagcact acgcgacggc cgcgaagctc atacgcaagc tcgtcgagcg caacatcacg
      181 gatcccctgc cgacggacgt cattctgaac atcaacgttc ccgacgtgcc gtacgatcag
      241 ctcaaaggcg tgcgtgcgac gcgtctcggc ttccgccaca agtccgagcc ggccgtgaag
      301 gcgctcgacc cgcacaaccg cccgatctac tggatcggcc cggccggcgc gggccaggat
      361 acgggccccg gcacggactt ccacgcgatc gcgcaagggt tcgtctcggt gtcgccgatc
      421 aaggtcgatc tcacggcgca cgcgtcgatc gaaaaggtcg cgacgtggtt cgagcggttc
      481 cgatgaacgg agcgacgcgc aacggcatcg gcatgacgtc ggcgcgcacg cgcgaccggc
      541 tcatcgagcg gctcaaggcc gaaggcatca cgaaccacgg ggttctcgag cgtgtgcggg
      601 ccgtgccacg ccatctgttc gtcgacgagg cgctcgcgag ccgcgcgtac gaggacaccg
      661 cgttgccgat cggcttcggc cagacgatct cgcagccgta catcgttgcc gcgatgacgg
      721 aagcgctcat cgaaagcggc aagaagctca cgcgcgtgct cgagatcggc acgggctgtg
      781 gttatcaaac cgccgtgctg gcgccgttcg tgcagcacat ctacagcgtc gagcgcattc
      841 agccgctcgt gcagcgcgcg cgcggcatgc tcgcggaatt gcgcatcacg aacgtcagcc
      901 tgcggcacgc cgatggttgg cacggctggc gcagccaagc tccgttccaa ggaatgatcg
      961 tcgcggccgc gccggagatg gttccgcccg agctttacga gcagctcgac gaaggcgggc
     1021 ggctcgtgat tccgatcggc gcgcgcggcc ggcagacgct gctgcgcgtg acgcgcaacg
     1081 gcgacgagtt caagcgcgag aacctgctgc cggtcagctt cgtgccgttc gtcgaaggca
     1141 cggagacatg aatcccggcc gcaagagacg ccgcaacggc ggcgcctccg cggccgcaaa
     1201 ttgctcctgc attttgcggc acttccgcca tccatggcgg tcggcggtcc ggctctgcgc
     1261 gtcgtggttg tgcgtggcgc tggtcgtggc gtgcacgacc gcgagcaacc cgccgctgag
     1321 ccggcgcgat gcgcgcgcgg ccgtgcacgt cgtgcagcgc ggcgaaaccc tgtcgaagat
     1381 cgcctggcag caccgcgtcg acccgcaaga gctcgccaat tggaacggag tcaccgatcc
     1441 cgatgcgctg cgcattggtc aacggctgcg gctcgtgccg ccgcgcggct acgtcgcgct
     1501 gccgactccg ccgcctgtcg cgcgaaccgc gcccaacgcg gctccgcctc cggcgccgac
     1561 tcgcgcaacg ccgccgaggt catcgtcggc accgcgctct tcgacgacgg cgccgcagca
     1621 gcgtccgtcc gcggcgcctc cggcgcggcc ttcgtcgacg cccgcgcgac agagcgcgcc
     1681 gccgcctgcg caagcatcgc cgccgcgctg gtcgtggccg accgacggcc gcatcgtcgc
     1741 gggtttcggc tcggccgacg gcatcgagtc cggcatcggc atcgcgggac gcgaaggcca
     1801 acccgttcgg gctgcagccg cggggcgagt cgtctacgcc ggcggcggtc tcatgggtta
     1861 cgggcagctc gtcatcatca agcacgacga gacgttcttg agcgcgtacg gctacaactc
     1921 ggagttgctc gtcacgcaag gccaagacgt cgtgcgcggc gcgacgatcg cgctgatggg
     1981 ccaagggccg ggccggcaag cgcgcttgca cttcgagatc cgccgcaacg gcgtgcccgt
     2041 cgatccactg cttttcgtca ccgcacctcg ctagctcatc gtagcgttgg ggaattttcg
     2101 cggcgcgcgc gaggttccgc tgttgcggat gaaaggcgaa gtcccggtaa cgagtcgaac
     2161 gcttggagtt ctcgccgtgc gtgcgagagc ccgtgcggcg gcaatagcca caagccgagc
     2221 tgccgcagct gtcacgtgga taggccccgc gcgcagcgcg gggtgcgcgg cagggatgcc
     2281 gcgcacgcgc agcgcgggca ggatgcccgc cctgcgcgcc ccacgtgac
//
LOCUS       DATLEE010000793         2931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6629918, whole genome shotgun sequence.
ACCESSION   DATLEE010000793 DATLEE010000000
VERSION     DATLEE010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6629918"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1094)
                     /locus_tag="VM692_17340"
     CDS             complement(108..1094)
                     /locus_tag="VM692_17340"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HUQ53997.1"
                     /translation="MPLRAWQYGAALAVIAIGLPLHAQSSDPHSVSAEQYARWKTELS
                     NWGRWGAADEIGALNLITTDKRRQAAALVRDGVSVSLAADADTVKAVDNPNPYEVQML
                     GIQADRISVEYHGIAHTHLDSLAHINEQGVFYNNYRPEADTVLRERHARNSIHNVKNG
                     VFTRGLLIDIPRLKGVPWLEPGTPIYVEDLEAWEERSGVKVSAGDALFVRTGVWARRK
                     AVGPWLRGRAEGGQSAGLHPSVIPWLKARDIALLGSDHPQYVSPGTPGAPRGAVHDFA
                     LLYLGVHLFDNCDLEALAEAAAARGRWEFLLTAAPLPIPGGTGSPLNPIATF"
     gene            1255..1785
                     /locus_tag="VM692_17345"
     CDS             1255..1785
                     /locus_tag="VM692_17345"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUQ53998.1"
                     /translation="MTLVSRARYAAFAMLMSAMAPLCAQAPAVAASADPAAAEVLELE
                     REIGAAVVRGDTKFFDEVTSPDFVMLHGDAWTHGGEPALVDDKASFSRRVASKSYAVL
                     DYEVQAAEMHGGVAITYGRYVGHIPSSPPDRRWFYVWYQKVYAKRAGRWLYLSHRTVD
                     GAHYARDRESLHSARQ"
     gene            complement(1795..>2931)
                     /locus_tag="VM692_17350"
     CDS             complement(1795..>2931)
                     /locus_tag="VM692_17350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUQ53999.1"
                     /translation="ILLLVGSALALLAWLAVLSAPHQPHRARERLEADPAAADDLSSV
                     NVLIPARDEAAVIGRTVAALHAQGRRLEVVVIDDQSSDDTRGAATRVARPDLPLRVLA
                     GRPLPEGWAGKLWALEQGLAVVERPYVLLLDADIELAPHTVPALLAAALERDATLVSL
                     LAELHCTTLWEKLLSPAFVLFFKLLYPFAWSNDARKATAAAAGGCMLVRTDVLREIGG
                     FAAIRGALIDDCTLAAALKHSRPPIWLGMTHSVRSLRVYATLDDFWRMVSRSAFTQLR
                     YSTAWLLAATLLMGVTLLVPVAGVAAGIVAGDRWLLASAAAAWLATGAVYWPVVAFYR
                     LSPAWAATLPVAAVLFLAMTWSSAIGYWRGTRASWKARDYARPS"
BASE COUNT          487 a         1010 c          999 g          435 t
ORIGIN      
        1 gggatgtgcg ccgacgagcc ccagggacgg tcttccggcg tgtctcgcga gaggcctcga
       61 tcgctcggac aggctcccag aaggagtgat gttccgaccg gcgagcttta gaacgtcgcg
      121 atcggattca gaggcgaacc ggtgccgccc ggtatcggca gcggcgcggc cgtcagcagg
      181 aactcccagc ggccgcgcgc ggcggcggct tcggccagcg cttcgagatc gcagttgtcg
      241 aacaggtgca cgccgagata gaggagcgcg aagtcgtgca cggcgccgcg cggcgctccc
      301 ggcgtgccgg gcgaaacgta ctgcgggtga tcgctgccca agagcgcgat gtcgcgagcc
      361 ttgagccacg gaatgaccga cggatgcagg ccggccgact gcccgccctc ggcgcggccg
      421 cgcagccacg gcccgacggc cttgcgccgc gcccacacgc ccgtgcgcac gaacaatgcg
      481 tcgccggccg agaccttgac gcccgagcgc tcttcccacg cttcgagatc ctccacgtag
      541 atcggcgtgc cgggctcgag ccatggaacg cctttgaggc gcgggatgtc gatcaatagg
      601 ccgcgcgtga acacgccgtt cttcacgttg tggatcgagt tgcgcgcgtg acgctcgcgc
      661 aagaccgtgt ccgcttcggg gcggtagttg ttgtagaaca cgccctgctc gttgatgtgc
      721 gcgagcgaat cgagatgcgt gtgagcgatg ccgtggtatt cgacggaaat gcgatccgcc
      781 tggatgccga gcatctgcac ctcgtaggga ttcggattgt cgacggcctt cacggtatcc
      841 gcgtcggcgg ccaacgacac cgacacgccg tcccgcacga gcgccgcggc ctggcgacgc
      901 ttgtccgtcg tgatgaggtt cagcgcgccg atctcgtcgg cggcgcccca gcggccccag
      961 ttcgacagct ccgttttcca gcgcgcgtac tgttccgcgc tgacgctatg tgggtcgctg
     1021 ctctgagcgt gcagcggcag gccgatcgcg atcaccgcca gcgccgcacc atattgccaa
     1081 gctcgtaacg gcatcgaagt actctcccct tgtaatggcc cgtgatcacc aagcgcggct
     1141 cgtcgagggc tagcctcgca tcgccggccc tcggttagca atcgaccgcg gtggaccctg
     1201 tcccggccgc gtgctccaat cgccgtggtg cggcgcgcag tcgggagatc tgccatgacg
     1261 cttgtctcga gggctcgcta cgcagcgttc gcgatgctga tgagcgcgat ggcgccgctg
     1321 tgtgcacagg caccagccgt cgctgcgtcc gcggatccgg ccgccgcaga agtgctcgag
     1381 ctcgagcggg agatcggcgc tgccgtcgtg cgaggcgata cgaaattctt cgacgaagtg
     1441 acttcgcccg acttcgtcat gctgcacggc gatgcctgga cgcacggtgg cgaacctgcg
     1501 ctcgtcgacg ataaggcgag cttctcgcgc cgcgtggcga gcaaatcgta cgcggtgctc
     1561 gattacgagg tgcaagcggc ggagatgcac ggcggcgtcg ccatcaccta cggccgctac
     1621 gtcggacaca tcccaagctc gccgccggat cggcgctggt tctacgtgtg gtatcagaag
     1681 gtttacgcca agcgcgccgg gcgctggctg tacctgtcac atcgaacagt cgacggcgcg
     1741 cactatgcgc gcgaccgtga gtccctgcac agcgcacggc agtagccgct gcggttagct
     1801 cggccgcgcg tagtcgcgcg ctttccagct cgcgcgcgtg ccgcgccaat agccgatagc
     1861 cgagctccac gtcatcgcga gaaacaacac cgcggcaacc ggcaaggtcg ctgcccatgc
     1921 agggctcaag cggtagaacg cgacgacggg ccagtagact gcgcccgtcg cgagccatgc
     1981 ggccgccgcc gacgcgagca gccagcgatc acccgccacg ataccggccg cgacgcccgc
     2041 gaccggcacg agtagcgtca cgcccatcag cagcgtcgcg gcgagcagcc aggccgtcga
     2101 gtaacgcagc tgcgtgaatg cgctgcggct gaccatgcgc cagaagtcgt cgagcgtcgc
     2161 gtagacacgc aaactacgta ccgagtgcgt catgccgagc cagatcggtg gccggctgtg
     2221 cttcagcgcg gccgccaacg tgcaatcgtc gatcagcgcg ccgcggatcg ccgcaaagcc
     2281 gccgatctcg cgcagcacgt cggtgcgcac caacatgcag ccgcccgccg cggcagccgt
     2341 cgccttgcgc gcgtcgttgc tccaggcgaa cggatacagc agcttgaaga acagcacgaa
     2401 cgcggggctc agcaatttct cccaaagcgt cgtgcagtgc agctcggcca gcagcgacac
     2461 gagcgtagcg tcacgctcga gcgcggccgc cagcagcgcc ggcaccgtat gcggtgcgag
     2521 ctcgatatcg gcgtcgagca gcaagacgta gggccgctcg acgacggcga ggccctgctc
     2581 gagcgcccaa agtttgccgg cccaaccctc gggcagcggc cggccggcca gcacgcgcaa
     2641 cggcaggtcg ggtcgtgcga cgcgcgtcgc ggcgccgcgc gtgtcgtcgc tcgactgatc
     2701 gtcgatgacg acgacctcga gccggcgacc ctgcgcgtgg agcgccgcaa ccgtgcggcc
     2761 gatcacggcg gcttcgtccc gagccgggat cagcacgttc acggagctca agtcgtcggc
     2821 cgcggccgga tccgcttcga gccgctcgcg ggcgcgatgc ggctgatgcg gcgccgatag
     2881 cacggcgagc cacgccagca gcgccagcgc gctgccgacg aggagcaaga t
//
LOCUS       DATLEE010000794         5783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6665669, whole genome shotgun sequence.
ACCESSION   DATLEE010000794 DATLEE010000000
VERSION     DATLEE010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6665669"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VM692_17355"
     CDS             <1..1615
                     /locus_tag="VM692_17355"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HUQ54000.1"
                     /translation="VDNLRAARAADGGALLLLDAGDTFQGGIESNLSEGLMVVDAYNA
                     LGYTALAVGNHDFDFGSVDPRSHVAEWLPHALAGADLGDMQGALKAAAARARFPFLAA
                     NILDAATQRPVQWPNVAPSTIVTAAGVPLRGLPRGVRIGLLGMMTETGLRQTLAAHAV
                     GLTTSPLAATAAREAASLRERGAELVVLVTHAGGWCGETANPRDLSSCDDESEIFRLV
                     RELPRGTLQAVVAGHTHGTVAHFVDDVPIISVPNFGVQFGRMDLTFDTALRGVSGVHI
                     YEPQRVCSRVEPASGECAVAPAGVAAVYEGSPVVPSANVAAAIEPELTRVRALRAEPL
                     GVIADSAVARGTGNESALGNLFADAVRAAVPEADAALGYGAGRGGIRTDLPAGPLTFG
                     QAYDTFPFDNRITRVELTGAQLERVIALQVPFWIDGRRGLPGLAGIRVALRCDGQEPR
                     VRLTRESGKSVARDEALVVAMASNTVGRFAATAADGEPTIVAVELPVLVRDALAAWLH
                     AHGGHISADDFVAAPRWELPATGCNTESN"
     gene            complement(2018..2263)
                     /locus_tag="VM692_17360"
     CDS             complement(2018..2263)
                     /locus_tag="VM692_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ54001.1"
                     /translation="MEYLRVLQERERVLSFRHTESPTTRQFQTGPLCRDQLLTLLGMS
                     LGLGEMLQKNFAMHDFLADYVLMRGLPDILPIRGRPS"
     gene            complement(2405..2797)
                     /locus_tag="VM692_17365"
     CDS             complement(2405..2797)
                     /locus_tag="VM692_17365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HUQ54002.1"
                     /translation="MKLTFVNPRDVHAPVGPYTHTAVVPAGTELVFVAGQVGMRVDGT
                     VPATFAEQAELTFANLRACLAAHGLGVEAVVKLTVFVVPPQDFAVLRAVREKHFGSHR
                     PTSTTVYVPALAAPALMLEVEAVAAKGA"
     gene            complement(2799..3173)
                     /locus_tag="VM692_17370"
     CDS             complement(2799..3173)
                     /locus_tag="VM692_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015165858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HUQ54003.1"
                     /translation="MSDKTYRGGCHCGRVRFEVDAAERLEVLDCNCSLCGRTGYLHLI
                     VTKPQFRLVAGRDALTSYRFNTGAAQHLFCSVCGIKSFYVPRSHPDGYSVNARCLEGV
                     ELADLTLTPFDGRNWERAAGAL"
     gene            3371..4243
                     /locus_tag="VM692_17375"
     CDS             3371..4243
                     /locus_tag="VM692_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ54004.1"
                     /translation="MATIRPLYRCGILYLCLQAFALSAQMQRCSGSLQIGNDLAIAGR
                     CELSNTEIRGDVTLFAGGSLTARNVRIRGHLDGTRGDFVDLLDSRVDGHVRLQEFVGD
                     LTSIETTEIHGDVRLTNNRSRLEVLNNDIRNDMQANSNTGGVEISGNVFDKDLECNGN
                     TPAPTGVGNKIEGDSEGQCERLEAHAPDPTPPPPPPAPTPTPTPTPTPTPTPTPTPTP
                     TPTPTPTPTPTPTPTPTPPPTSSPPPATPTPVAPVDPLADEGGGAGAVNWPAVVFLLP
                     LLVWRRFARRPQPR"
     gene            4341..>5783
                     /locus_tag="VM692_17380"
     CDS             4341..>5783
                     /locus_tag="VM692_17380"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUQ54005.1"
                     /translation="MDTLFQDLKQALRLFRDLPAFAATAVLALTLGIGVNTAIFSVLN
                     AVLLKRLPYPEPERLVALLQADNGVPAAIVVTPAQFTHWQSLDDVFADVVAWRTVSFD
                     YSVDETPASVTAGTVSDGYFRTLGATFSAGRALAAEEHVAGAANAVVLSHSFWTRRLG
                     ARPDAIGSTLTLNGVPYTIVGITSAEFDVRGVDVRGFGVPDIWVPLHVDPDLTDFSVT
                     LDVFARLRDGVSLTAAQQRLAASIAAYRERYPADAEDWQFTALGLEETIVRGARPMLL
                     TLSGAVALVLLVACANVANLLLVRGASRSREVAIRATLGAGRARIVRQLVTESLVLTL
                     TGGALGLAAGIVCVRWVLASGLVDLPRVDSTATLLALDWRVAMFTLLLSIATGLVFGL
                     VPALVGARADLSSVMKDSTSRAAGGRSQTKTQTTLVVVEVALAVVLVIGAGLLIRTSL
                     AIGAVDLGLNLHDVLTLRSPADPAQPTADVA"
BASE COUNT          913 a         2040 c         1904 g          926 t
ORIGIN      
        1 cgtcgacaat ttgcgcgccg cgcgcgcggc cgacggcggc gcgctgctgc tgctcgacgc
       61 cggcgacacg ttccaaggcg gcatcgagtc gaatctctcc gaaggcctca tggtcgtcga
      121 cgcgtacaac gcgctcggct atacggcgct cgcggtcggc aatcacgact tcgacttcgg
      181 ctccgtcgat ccgcgcagcc acgtcgccga atggttgccg catgcgcttg cgggcgcgga
      241 cttaggcgac atgcaaggcg cgctgaaagc cgcggccgcc cgcgcacgct tcccgttcct
      301 cgcggccaac atcctcgacg cggccacgca gcggcccgtg cagtggccga acgtcgcgcc
      361 gtcgaccatc gtcaccgcgg ccggcgtccc ccttcgggga ctaccaaggg gcgtgcgcat
      421 cggcctgctc ggcatgatga ccgagacggg cttgcggcaa acgctcgctg cgcacgccgt
      481 cggcctcacg acgagcccgc tcgccgcgac ggcggcgcgc gaagccgcgt cattgcgcga
      541 acgcggcgcc gagctcgtcg tgctcgtgac gcatgccggg ggctggtgcg gcgaaactgc
      601 gaacccacgc gacttgagct cgtgcgacga cgagagcgag atcttccgcc tcgtccgcga
      661 gttgccgcgc ggcacgttgc aggcagtcgt ggccggccat acgcacggca cggtcgcgca
      721 tttcgtcgac gacgtgccga tcatctcggt gccgaacttc ggcgtgcaat ttggccggat
      781 ggatctgacg ttcgacaccg ctctgcgcgg cgtcagcggc gtgcatatct acgagccgca
      841 gcgcgtctgc tcgcgcgtcg agcccgcgtc cggcgaatgc gccgttgcgc cggccggcgt
      901 cgccgcggtt tacgaaggca gccccgttgt gccgagcgcc aacgtcgccg ctgcgatcga
      961 gcccgagctc acgcgcgtgc gcgcgctgcg cgccgagccg ctcggcgtca tcgcggactc
     1021 tgctgtagcg cgcggcacgg gcaacgagtc cgcgctcggc aacttgttcg ccgacgccgt
     1081 gcgcgccgcg gtgcccgaag ccgacgccgc gttgggttac ggcgccggcc gcggcggcat
     1141 ccgcaccgat ttgccggcgg gcccgctgac gttcggccag gcgtacgaca cgttcccgtt
     1201 cgacaaccgc attacgcgcg tcgagctcac gggcgcacaa ctcgagcgag tgatcgcgct
     1261 gcaggtgccg ttctggatcg acggccgccg cggcctgccc ggtctcgccg gcattcgcgt
     1321 cgcgctgcgc tgcgacggcc aggagccgcg cgtgcggctg acgcgcgaat ccgggaagtc
     1381 ggtcgcgcgc gacgaagctc tcgtcgtcgc gatggcttcg aacaccgtcg gccggttcgc
     1441 cgcgacggcg gccgacggcg agccgacgat cgtggccgtc gagctgcccg tgctcgtgcg
     1501 cgacgccttg gccgcgtggc tgcacgccca cggcggccac atcagcgccg acgacttcgt
     1561 cgcagcgccg cgctgggagc taccggcgac gggctgcaat actgaatcga actgaactct
     1621 aggtcttgct cagcccgcga atcagtcgcc atggcccaag aagtgatcgc tcgtcgccgc
     1681 ccttcgtcaa tgacctcagt gagcggacgc ttgcagtaac agacgagggc ccgcggacgt
     1741 gcgttgttgg taagacgccg attcttcgat gatctcgcga atgctgccga cgtacgctcg
     1801 tcgcgccccg ctcagcgtaa ctctggcatc gtcttccgtc tgccattcag cggcggttgc
     1861 gccacctcgt cgccgtatcc ctcggtcgag ggctcggccc gcaacatgat aagcgcgtat
     1921 cagggtctcg atttcttcta gcattgcgtc acctctagtg cacggtgcgc ctcttagccc
     1981 ggaatagata tggaagcggc tgacggccgc cagctagcta gcttggacgg ccgcggatag
     2041 gcaagatatc cggtaatccc cgcatcagaa cgtagtcggc aaggaagtca tgcatcgcga
     2101 aattcttctg gagcatctcg cccaagccga gagacatgcc gagcagggtc agaagctgat
     2161 cacggcacag cgggcccgtc tggaactggc gcgtggtcgg ggactcagtg tgacggaaac
     2221 tgagcacacg ctcgcgctct tggaggacac gcagatactc catgtcgctg atcgcgaccg
     2281 gctccgtgcc gagctacagg cgcttgcata ggggcgctgc ctaagcgggt aacgtgacga
     2341 acttcggaac aggtcgcgcg aagtcgcgta atttccgggg ggagccgagc gttgccaaca
     2401 agagtcaggc gcccttggcc gcgacggcct cgacctcgag catgagcgca ggcgccgcca
     2461 acgcaggcac atacaccgtc gtcgacgttg gccgatgact gccgaagtgt ttctcgcgca
     2521 ccgcgcgcaa caccgcgaag tcctgcggcg gcacgacgaa caccgtcagc ttcacgacgg
     2581 cttcgacgcc gagaccgtgc gcggcgaggc acgcgcgtag attcgcgaac gtcagctcgg
     2641 cctgctcggc gaaggtcgcg ggaaccgtgc cgtcgacgcg catccccact tggcccgcga
     2701 cgaacacgag ctcggtgccg gccggcacga cggccgtgtg tgtatacggg ccgacgggtg
     2761 catgcacgtc gcgcggattc acgaatgtca gcttcatctc acaacgcgcc ggcggcacgc
     2821 tcccaattgc gaccgtcaaa cggcgtgagc gtgagatcgg cgagctcgac gccctcgaga
     2881 caacgcgcgt tgacgctgta accgtcgggg tgcgaccgcg gcacgtaaaa cgacttgatg
     2941 ccgcaaaccg agcaaaacaa atgctgcgcc gcgccggtgt tgaaacggta gctcgtcagc
     3001 gcgtcgcggc cggccacgag acgaaactgc ggcttcgtga cgatcaagtg aagatagccg
     3061 gttctgccgc acaacgagca gttgcagtcg agcacctcga gacgctccgc agcgtcgacc
     3121 tcgaaacgaa cgcggccgca gtggcaaccg ccacggtaag tcttgtcgct cattgcgcgg
     3181 cactagacca tgcgcctcgc ggctcggtca attgagagcc gttcccatct cagcgccgca
     3241 gacgtacaga atctgcactc gattttcgct ccctgcaccg cggagaagcg ctgctaccgc
     3301 ccttcggatc gcaacgcgtt ggcggcccag gttttgcatc ttgttgagcg gctgtccacg
     3361 ggaggcgcga atggccacta tccgtccgct ctacaggtgc gggattctct atctttgtct
     3421 gcaggctttt gccctcagcg cgcaaatgca gcgctgctcg gggtcccttc agatcggtaa
     3481 cgatctggcc atcgcgggac gctgcgagct ctcgaacacc gagattcgag gcgacgtcac
     3541 gctgttcgcc ggcggctcgc tgaccgcgcg caacgtgcgc attcgcggcc acctcgacgg
     3601 gactcgcggc gatttcgtcg acctgttgga cagcagagtc gacggccacg tcaggttgca
     3661 agagttcgtc ggcgacctga cgagcatcga gaccaccgaa attcacggcg acgtccgcct
     3721 cacgaacaat cggtcgcgac tcgaggtcct caacaacgac atccgcaacg acatgcaagc
     3781 caacagcaat acgggcggag tcgagatctc gggcaacgtg ttcgacaagg acttggagtg
     3841 caacggcaac acgccggcgc ccacgggcgt cggcaacaag atcgaaggcg acagcgaagg
     3901 ccaatgcgag aggttggaag cccacgcgcc ggacccgacg ccgccgcctc ctccgcctgc
     3961 gccgacgccg actcccacgc cgacgccgac gccgacgccg acccccacgc cgaccccgac
     4021 gccgaccccg acgcccacgc cgacccccac cccgacgccc acgccgaccc cgacgccgcc
     4081 tccgacatcg tcgccgccgc ctgcgacgcc gacgccggtg gcccccgtcg acccgctggc
     4141 ggatgagggc ggcggcgcag gtgcggtcaa ctggccggcc gtagtgttcc tgttgccgct
     4201 gctcgtttgg cgccgcttcg cgcgccgccc gcagcctcgc tagcgctaaa gcgctcgacg
     4261 caccggaacc cgcacagccg gccgcctgtc tacgtggacg tgccggcgcc agccggcgcg
     4321 tcgcacaccg ggggttggcg gtggacaccc tgtttcaaga tctcaagcag gcgctccgcc
     4381 tgtttcgcga cctgccggcg ttcgcggcca cggcggtcct cgccctgacg ctcggcatcg
     4441 gcgtcaatac cgcgatcttc agcgtactca atgccgtgtt gttgaaacga ttgccctatc
     4501 ccgagcccga acgcctcgtt gcactactac aagcggacaa cggcgtgccg gccgcaatcg
     4561 tcgtcacacc ggcgcagttc acgcactggc agagcctcga cgacgtcttc gcggacgtcg
     4621 tcgcgtggcg cacggtgtcg ttcgattact ccgtcgacga gacgcccgcg agcgtcacgg
     4681 caggcaccgt gtcggacggc tacttccgca cgctcggcgc gacgttcagc gccggccgtg
     4741 cgctcgcggc cgaggagcat gtcgccggcg ccgcgaatgc cgtcgtcttg agccacagct
     4801 tctggacgcg ccggctcggc gcgcggcccg acgcgatcgg cagtaccctg acgttgaacg
     4861 gcgtgccgta cacgatcgtc ggcattacga gcgccgagtt cgacgtccgc ggcgtcgacg
     4921 tgcgcggctt cggcgtgccg gacatctggg tgccgctgca cgtcgatccc gacctgaccg
     4981 atttcagcgt cacgctcgac gtcttcgctc gtttgcgcga cggcgtgtcg ctgaccgcgg
     5041 cgcagcagcg gctcgccgca tcgatcgccg cgtatcgcga gcgctatccc gcggatgcgg
     5101 aagactggca gttcacggcg ctgggcctcg aggaaacgat cgtgcgcggc gcgcgcccga
     5161 tgctgctgac gttgtcgggc gccgtcgcgc tcgtgctgct cgtggcctgc gcgaacgtcg
     5221 cgaatctctt actcgtgcgc ggcgcgagcc gcagccgcga agtcgcgatc cgcgcgacgc
     5281 tcggcgcggg ccgcgcgcgc atcgttcggc agctcgtgac cgagagcctg gtgctgacgc
     5341 tgaccggcgg cgcgctgggg ctcgcggccg gcatcgtctg cgtacgctgg gtgctcgcga
     5401 gcggcttggt cgacctgccg cgcgtcgact caacggcgac gctgctcgcg ctcgattggc
     5461 gcgtcgcaat gttcacgttg ctgttgtcga tcgccacggg cctcgtgttc gggctcgtgc
     5521 ctgcgctcgt cggcgcacgc gccgacttga gctccgtcat gaaggactcg acgagccgcg
     5581 cggccggcgg ccggagccaa accaagacgc agacgacgct cgtcgtcgtc gaggtcgcgc
     5641 tggccgtcgt gctcgtcatc ggcgccggct tgttgattcg cacctcgctg gcgatcggtg
     5701 ccgtcgacct cggcttgaac ctgcacgacg tgctgacgct gcgcagcccg gccgatcccg
     5761 cgcaaccgac ggccgatgtc gcc
//
LOCUS       DATLEE010000795         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6667135, whole genome shotgun sequence.
ACCESSION   DATLEE010000795 DATLEE010000000
VERSION     DATLEE010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6667135"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..374)
                     /gene="ftsL"
                     /locus_tag="VM692_17385"
     CDS             complement(87..374)
                     /gene="ftsL"
                     /locus_tag="VM692_17385"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HUQ54006.1"
                     /translation="MSRQVVELVGVLVLAACVVASGIWIVDVEHRSRQLFIETEGLSR
                     ELDRLQIEWGQLQIEQSTLGTHSRIEALARQRMHLTEPSDNQLVVVTESAR"
     gene            complement(399..1349)
                     /gene="rsmH"
                     /locus_tag="VM692_17390"
     CDS             complement(399..1349)
                     /gene="rsmH"
                     /locus_tag="VM692_17390"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HUQ54007.1"
                     /translation="MTSAPGHQPVLLQEALEGLSLRNGGWYVDATYGRGGHSAEILAR
                     LGPQGRLLALDKDADAVADAREQLGRDPRFTIEHAGFEELAAIARPWLGQRRLAGILF
                     DLGVSSPQLDVPQRGFSFAHDGPLDMRMNQTHGATAAEWLAAVAQHELADVLFRYGEE
                     PRARQIAAAIVRTRVTEPLATTRQLANLVAAHAGHQQKRIHPATRTFQAIRIAVNDEL
                     GALERGLAQSLALLDEGGRLVVISFHSLEDRIVKRFMAREARGDEAYAGLPNIPPHAR
                     PRLTLVGKLVRPGAAELERNPRGRSARLRVAERTPLEQAA"
     gene            complement(1363..1815)
                     /gene="mraZ"
                     /locus_tag="VM692_17395"
     CDS             complement(1363..1815)
                     /gene="mraZ"
                     /locus_tag="VM692_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HUQ54008.1"
                     /translation="MFRGATKVTLDAKGRMAIPARYRERLLTVGGKVVATVDRDHCLL
                     IYPLPDWEEIERKLMRLPTLNKQVRRLQRLMLGYATELEMDGHGRILLTRELREFAGL
                     ERQAMLIGQGNKFELWSDERWGKRRDEWLADDEGEQAGLPQELESLSL"
BASE COUNT          344 a          697 c          623 g          361 t
ORIGIN      
        1 cacacgcggc gaagcccgcc agcagcaggg cgctgcgcca gcgcgcgcct cgggcgactg
       61 tcgttttcgc ggttcgcttc atctcttcaa cgcgccgact cggtcacgac gacgagctgg
      121 ttgtcgctcg gctccgtgag gtgcattcgc tggcgcgcga gcgcctcgat gcgcgaatgc
      181 gtgcccaacg tgctctgctc gatctgcaat tgcccccact cgatctgcag gcggtcgagc
      241 tcgcggctca agccttcggt ctcgatgaac agctgccgcg accgatgctc gacgtcgacg
      301 atccagatgc ccgaggccac gacacaagcg gccaacacga gcactccgac gagctccacg
      361 acttgtctac tcattcgggt ctactcattc gcggctgctc atgcggcctg ttcgagcggc
      421 gtgcgctcgg cgacgcgcaa ccgtgcgcta cgcccacgcg gattgcgctc aagctccgcg
      481 gcgccgggcc gcacgagctt gccgacgagc gtgagtcgcg gccgagcgtg cggcggaatg
      541 ttcggcagcc ccgcgtacgc ctcgtcgccg cgcgcttcgc gcgccatgaa acgcttgacg
      601 atgcgatcct cgagtgaatg gaagctgatc acgacgagcc gcccgccctc gtcgagcagc
      661 gcgaggctct gcgcgagtcc gcgctcgagc gcgccgagct cgtcgttgac ggcgatgcga
      721 atcgcctgga acgtccgcgt cgccgggtga atgcgcttct gttggtggcc ggcgtgcgcg
      781 gcgacgagat tggcaagttg gcgcgtcgtc gccagcggct cggtcacgcg ggtgcgaacg
      841 atcgccgcgg cgatctgccg cgcacgcggc tcttcgccgt aacgaaacag cacgtcggcg
      901 agctcgtgct gcgcaacggc agcgagccac tccgccgccg tcgcgccatg cgtctgattc
      961 atgcgcatgt ccaggggtcc gtcgtgcgca aaactgaagc cgcgctgcgg cacatcaagc
     1021 tgcggtgaag agacgccgag atcgaacaga atgccggcca atcgccgttg cccgagccaa
     1081 ggccgcgcga tggcggcaag ctcttcgaag ccggcgtgct cgatggtgaa tcgcgggtca
     1141 cggccgagct gctcgcgagc gtcagccacc gcgtcggcgt ctttgtccaa agccagtaat
     1201 cgtccctgtg gtccaagccg cgccaaaatt tcagcgctgt gcccgccgcg tccgtaggtc
     1261 gcatcgacgt accaaccgcc attccgcaac gacaaacctt cgagcgcttc ctggagcagc
     1321 acgggctgat gtccaggtgc gctggtcacc ggcggtaatg ctctacagag acagcgattc
     1381 gagttcttgt ggtaggcccg cttgttcgcc ttcgtcgtcc gccagccatt cgtcgcggcg
     1441 cttgccccaa cgctcgtcgc tccacagctc gaacttgttt ccctggccga tcagcatcgc
     1501 ctgccgttcg aggcccgcga actcgcgcag ctctcgcgtc aacaggatcc gcccgtgacc
     1561 gtccatctcg agctcggtcg cgtaaccgag catcaaacgc tgcaaacgcc gcacctgttt
     1621 attcagggtc ggcaatcgca tgagcttgcg ttcgatctcc tcccaatcag gcagtgggta
     1681 aatgagtagg cagtggtcac gatcgacggt cgccaccact ttgccgccca cggtcagcag
     1741 gcgttcccgg taccgcgccg ggatggccat ccgacccttc gcatcgaggg tgactttggt
     1801 agcgccgcga aacataacca ggctgccggc cccattccat cccacttttc tccacttcgt
     1861 gacactatat tgacagccac tgcgcaggtc aacgaaaacc cgccaaaaaa atgagaactg
     1921 gccagcattg agcgaagtag aacgtctgcc ttcagcgaca cgcgtttcag tgcagcgtgc
     1981 gcagcgtcgg acttctcggc cgcgagtcgg tcgagggccg ttccc
//
LOCUS       DATLEE010000796         2745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6686608, whole genome shotgun sequence.
ACCESSION   DATLEE010000796 DATLEE010000000
VERSION     DATLEE010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6686608"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VM692_17400"
     CDS             complement(<1..447)
                     /locus_tag="VM692_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUQ54009.1"
                     /translation="MAARKTGASRSGARKGATTAPQANAPAWLDVAADYYWEQDAEHR
                     FTVWRSTDRKRAGVVEAATLLGKTSAELCAAPGDDTDHWVRHRAVLEARGPFRDVAHA
                     LPLRSGAVGNLSLSGAAAYDAEGAFVGYRGVARDTGGYARLTRLLAL"
     gene            complement(550..1413)
                     /locus_tag="VM692_17405"
     CDS             complement(550..1413)
                     /locus_tag="VM692_17405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUQ54010.1"
                     /translation="MATRIGFALAAVASLAAVWAAPAVAELSNKAAPVIVGHYHLNVT
                     SIADHKRFWVDTLGGKATKIGNVDAIEFPDVYLLLHEGKPTGPTRGTTFDHIGFAVPD
                     VPAMTTKVVANGYGLTVGREPAPGETASPPTAGNYGRFSYLVGPDGVKVELVTNMASD
                     APPIIHHHVHFVNKQFVEMQQWYMKAFNATLRDGQTDFFIGADLPGVGYMLNFFSWLP
                     NENLIGTAGRAVDHVGFEVRGLRAFCAELERKGLKLTAPYRRDESLGLATATIVDPWG
                     TVLELTEGLRK"
     gene            complement(1500..>2745)
                     /locus_tag="VM692_17410"
     CDS             complement(1500..>2745)
                     /locus_tag="VM692_17410"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUQ54011.1"
                     /translation="NGNVQAATLLLDAGADVNAIEAWGQQSPLMWAAAQKQPAMIKLL
                     VARGADVNAHGAVRNWERKVIREPRPKDMNQGGFTPLLYAAREGCIECAKELVAGGAD
                     LDLPDPHRVTPLIMAMLNLHFDFAAYLIEAGADVDKWDLYGRSPLYMAADTSTIPVMG
                     NGAMVVLPSMDKLKAIDIAKLLLDKGANVNAQLKRRPPYRNVPQDRGGDSILSQGATP
                     LLRAARAGDTPMVQLLLERGALVDLASNQGVTPLMAAAGVEFGLRVTRGRNRTEEGVL
                     STMRALLDAGANIDARMLVEPRGESAAHLLVIEQRLSDYGYDYRGRQVPSLRAVPHRT
                     ALHGAAMKGFTSIVKFLAENGADLYAKDANGSTALDLAEGNYNEPFLRQAAEPHADTA
                     AVLKELMAANPPPAAQSTAAAR"
BASE COUNT          426 a          921 c          924 g          474 t
ORIGIN      
        1 gagcgcgagc agacgcgtca gccgcgcgta gcccccggta tcgcgcgcaa cgccgcggta
       61 gccgacgaac gcaccctcgg cgtcatacgc ggctgcgccg ctaagactca agtttccgac
      121 ggcgccggag cgcagcggca acgcgtgcgc gacgtcgcga aacggccccc gcgcttcgag
      181 cacggcgcgg tgtcgaaccc agtggtcggt gtcgtcgccg ggagcggcgc agagctcggc
      241 gctcgtcttg ccgagcaacg tggccgcttc gacgacgccg gcgcgcttgc gatcggtgct
      301 acgccaaacc gtgaagcgat gctcggcgtc ctgctcccag tagtaatcgg ccgcgacgtc
      361 gagccaagca ggcgcgttgg cctgaggcgc ggtggttgca cctttacgtg caccgctgcg
      421 cgacgcgcct gtcttcctcg ctgccatcgc cgtcccacga caccagaacc ccaagcagga
      481 taacgggcgc gacttcgctg cggcccttgc gcgcgtctca attttcgacg cgcttcgcgc
      541 gtcgcgagcc tacttgcgca gcccttccgt gagctcgagc accgtgcccc acggatcgac
      601 gatcgtcgcg gttgcgaggc cgagcgattc gtcgcgcctg taaggcgcag tgagctttag
      661 gcccttgcgc tcgagctcgg cgcagaaagc cctcaagcct cgaacttcga agccgacatg
      721 gtcgactgcg cggcctgcgg ttccgatgag attctcgttc ggcagccagc tgaagaaatt
      781 cagcatgtaa ccgacgccgg gcagatcggc gccgatgaag aagtcggttt gtccgtcgcg
      841 cagtgtcgcg ttgaaggcct tcatgtacca ctgctgcatc tcgacgaact gcttgttcac
      901 gaagtgcacg tggtgatgaa tgatcggcgg cgcgtcggag gccatgttcg tcacgagctc
      961 gactttgacg ccgtcggggc cgacgagata cgagaaccgg ccgtagttgc ccgcggtcgg
     1021 cgggctcgcc gtctcgccgg gcgccggctc gcggccgacc gtcaggccat agccgttggc
     1081 gacgaccttc gtcgtcatgg ccggcacatc ggggacggcg aagccgatgt gatcgaacgt
     1141 cgtgccgcgc gtcgggcccg tcggcttgcc ttcgtgcaac agcagataga cgtcgggaaa
     1201 ctcgatcgcg tcgacgttgc cgatcttggt cgctttaccg ccgagcgtgt cgacccagaa
     1261 gcgcttgtgg tcggcgatgc tcgtcacgtt caagtggtaa tggcccacga tgacgggcgc
     1321 cgctttgttg gagagctcgg cgactgcggg agctgcccat accgccgcaa gcgacgctac
     1381 cgcggccaac gcgaacccga ttcttgtcgc catcttcgtt cccccaagag ccgctatgaa
     1441 gcagcgatct taagcgaacg cccggcggcc gacctagcgc gcagcggcca aagctgacgc
     1501 tagcgcgcgg cggccgtgct ctgcgcggcc ggaggcgggt tcgccgccat aagctctttg
     1561 aggacggcgg cggtgtccgc gtgcggctcg gcggcctggc ggaggaacgg ctcgttgtag
     1621 ttgccttcgg cgagatcgag cgccgtgctg ccgttcgcgt ccttggcgta gaggtccgcg
     1681 ccgttctccg cgagaaactt cacgatcgag gtgaagcctt tcatcgcggc gccgtgcaac
     1741 gccgtgcgat gcggcacggc acgcaggctc ggcacttgcc ggccgcgata gtcgtagccg
     1801 tagtcgctga ggcgctgctc gatcacgagc aagtgcgcgg ccgactcgcc gcgcggctcg
     1861 accagcatgc gcgcgtcgat gttggcgccc gcgtcgagca gcgcgcgcat cgtcgatagc
     1921 acgccttcct cggtgcgatt gcggccgcgc gtgacgcgta acccgaactc gacgccggcg
     1981 gccgccatca acggcgtaac gccttggttg ctcgcgagat cgacgagcgc gccgcgctcg
     2041 agcagcagct gcaccatcgg cgtgtcgccg gcgcgcgcgg cacgcagcaa cggcgtggcg
     2101 ccttgcgaaa gaatcgagtc gccgccacga tcctgcggca cgttgcggta cggcggccgg
     2161 cgcttcagct gcgcgttgac gttggcgccc ttgtcgagca gcagcttcgc aatgtcgatg
     2221 gccttgagct tgtccatgct cggcagcacg accatcgcgc cgttgcccat gacgggaatc
     2281 gtgctcgtgt cggcggccat gtagagcggc gagcgaccgt agaggtccca cttgtcgacg
     2341 tcggcaccgg cttcgatcaa gtacgccgcg aaatcgaagt gcagattcaa catcgccatg
     2401 atcagcggcg tcacgcgatg cggatcgggc aagtcgaggt cggcgccgcc ggcgacgagc
     2461 tccttcgcac actcgataca gccttcacgc gcggcgtaca acagcggcgt gaagccgccc
     2521 tggttcatgt ccttggggcg cggctcgcgg atgacttttc gttcccagtt gcggaccgcg
     2581 ccgtgcgcat tgacgtcggc gccgcgggcg acgagcagct tgatcatcgc cggctgcttt
     2641 tgcgccgcgg cccacatcag cggactttgc tgcccccaag cttcgatcgc gttgacgtcg
     2701 gcgcccgcgt cgagcaacag ggttgccgcc tgcacgttgc cgttg
//
LOCUS       DATLEE010000797         2674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U14368
            k141_6702544, whole genome shotgun sequence.
ACCESSION   DATLEE010000797 DATLEE010000000
VERSION     DATLEE010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938847
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:09:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,485
            CDSs (total)                      :: 3,461
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6702544"
                     /isolate="SMAG_U14368"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="VM692_17415"
     CDS             complement(<1..1587)
                     /locus_tag="VM692_17415"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HUQ54012.1"
                     /translation="MRRVSLALGIGFVGGLLATTSPALAQQPAPAPTREQLEQSGATI
                     RAIYVTVDNIFDPNNPEEDKALYRWANKAHVRTRDSVVRDILLFAPGDKFEARLLDES
                     ARTLRARGFIADAAVEPGAYDAASNSVDVNVRVRDSWSLSLDLKLNRKGGQTEWGVGL
                     SDNNLLGTGKTLEVVHESEIDRDHTALGYVDNNVFGSRIQLRTSFRNASDGDRRQLAV
                     ERPFYALDTRWSLGGNLHSEERIDTMYDLGEEIDEFGHEINISTLQGGWSRGLVGKRA
                     ARWLAGYTSEEHTFSPTTDVPQPLLLPPNRKLVYPWVGWQLVEDDFREMTELNTVGRT
                     EDISLGLNVLASIGFADTSYDSDRNAELLRFDLAKGWEPRGSGLLLMQINGSTRREDV
                     GYRNSQASFSARYYQRNLERHLFLATLSVLETRHLDPENQVLLGGDNGLRGYPIRYQA
                     GESRTILSVEQRFFTDFYPWRLFRVGWAVFADAGRVGGRDPRATPSQGTLYDVGAGLR
                     LASPRASGRNVVHIDLAFPLN"
     gene            complement(1641..2255)
                     /locus_tag="VM692_17420"
     CDS             complement(1641..2255)
                     /locus_tag="VM692_17420"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUQ54013.1"
                     /translation="MSAAERDKWDARYREGAYEQRTHPTALLAEWLPRLRRPLEVPEG
                     GRGRALDVACGTGRNALYLAADGFDVTALDISSVALERGRGAATERGLDVEWLCADLD
                     DDVERALPSREFDLIVWVRYIHRTLMASLIARLAVGGTLLCEQHLATTEPAAGPTSAA
                     FRLAAGDLARTARPLHILHTYEGLIVDPDGRRVALAQLIGRRPA"
     gene            complement(2260..>2674)
                     /locus_tag="VM692_17425"
     CDS             complement(2260..>2674)
                     /locus_tag="VM692_17425"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUQ54014.1"
                     /translation="AKELGAFGTVNYRSNPDWQVEVRKLTGGTGVNQVLEVGGQDTLP
                     RALQALAFGGHLAIIGGLSGFASDVPVGTLMGRNATASGIYVGSRADFEALNAFLSEH
                     KIKPIVDKVFELEDAPAAFAAMDSGDFFGKVVIRL"
BASE COUNT          439 a          892 c          885 g          458 t
ORIGIN      
        1 gttcagcggg aacgcgaggt cgatgtgcac gacgttgcgg ccgctcgcgc gcggcgacgc
       61 caggcgcagg cccgcgccga cgtcgtagag cgtgccttgg ctaggcgtcg cgcgcggatc
      121 gcgtccgccg acgcggcccg cgtctgcgaa cacggcccag ccgacgcgaa acagtcgcca
      181 cggatagaaa tccgtgaaaa accgttgctc gacactcaag atggttcggc tctcgccggc
      241 ttgatagcgg atcggatagc cgcgcaggcc gttgtcgccg ccgagcagca cctgattctc
      301 ggggtcgagg tggcgtgtct cgagaacgct gagcgttgcc aggaaaaggt gtcgctcgag
      361 attgcgctgg taatagcgcg ccgagaacga agcttgcgag ttgcgatagc ctacgtcctc
      421 gcggcgcgtc gagccgttga tctgcatcag caacaggccg gagccgcggg gctcccaacc
      481 ttttgcgagg tcgaagcgca gcagctccgc attgcgatcg gaatcgtagc tcgtgtcggc
      541 gaagccgatg ctcgcgagca cgttgagacc gagcgagatg tcctccgtgc ggccgacggt
      601 gttgagctcc gtcatctccc gaaagtcgtc ttcgacgagc tgccagccga cccacggata
      661 cacgagcttg cggttcggcg gcagcagcag cggttgcggc acgtccgtcg tcggactgaa
      721 cgtgtgctcc tcggatgtgt agccggcgag ccaacgcgcc gcgcgtttgc cgacgagccc
      781 gcgcgaccac ccgccttgca gcgtcgagat attgatctca tgtccgaact cgtcgatctc
      841 ttcgccgagg tcgtacatcg tatcgatgcg ttcttcgcta tgaagattgc cgccgagcga
      901 ccagcgagtg tcgagcgcat aaaacggccg ctcgacggcc agctggcggc ggtcgccgtc
      961 gctggcgttg cggaacgacg ttcgcagctg tattcggctg ccgaacacgt tgttgtccac
     1021 gtagccgagc gcggtgtgat cgcgatcgat ctcgctctcg tggacgacct cgagcgtctt
     1081 gcccgtgccg agcaggttat tgtccgagag gccgacgccc cattcggtct ggccgccttt
     1141 gcgattcagc ttgaggtcga gcgacagcga ccatgaatcg cggacgcgca cgttgacgtc
     1201 gacactgttg ctggcggcat cgtaagcgcc cggttcgacg gccgcatccg cgatgaagcc
     1261 gcgcgcgcgt agcgtgcgtg ccgactcgtc gagcaatctc gcctcgaact tgtcgccggg
     1321 cgcgaacaac aagatgtcgc gaacgacgct gtcgcgtgtc cgcacgtgcg ccttgttcgc
     1381 ccagcggtag agagccttgt cttcctcggg attgttcgga tcgaagatgt tgtcgaccgt
     1441 gacgtagatc gcgcggatcg tggcgccgga ttgctcgagt tgttcccgag tcggcgcggg
     1501 agccggttgc tgcgccaatg cgggcgatgt cgtcgcgagc agaccgccga cgaaaccgat
     1561 gccaagcgct agcgagactc tgcgcatcgc tttggagctt accccgcgcg gtccgcgatt
     1621 agtgtgccgc gccacccaca tcaagccggc ctacgcccga tcagctgtgc cagcgcgacg
     1681 cgccggccat cgggatcgac gatcaggcct tcgtacgtat gcagtatgtg caaaggccgt
     1741 gccgtccgcg cgaggtcgcc ggcggcgagg cggaatgccg cgctcgtcgg tcccgcggcg
     1801 ggttcggtcg tggccaggtg ctgctcgcaa agcaacgtgc cgccgacggc caggcgcgcg
     1861 atcagcgacg ccatcaacgt gcgatggatg taacgcaccc agacgatcaa gtcgaactcg
     1921 cggctcggca gtgctcgctc cacgtcgtcg tcgaggtccg cacacagcca ttcgacgtcg
     1981 aggccgcgct cggtcgcggc gccgcgcccg cgctccagtg caaccgagga gatgtcgaga
     2041 gcggtgacgt cgaaaccgtc ggccgcgaga tacagcgcgt tccgcccggt gccgcacgcc
     2101 acgtcgagcg cgcggccacg ccccccttcg gggacttcca aggggcgacg caaccgcggc
     2161 agccactccg cgagcagcgc cgtcggatgc gtgcgctgct cgtaagcacc ttcacgatag
     2221 cgcgcgtccc acttgtcgcg ttccgcggcg ctcacgggct cacaagcgaa tgacgacctt
     2281 gccgaagaaa tcgccgctgt ccatggccgc gaatgcggcc ggcgcgtcct cgagctcgaa
     2341 caccttgtcg acgatcggct tgatcttgtg ctcgctcaag aacgcgttca acgcttcgaa
     2401 gtccgcgcgc gagccgacgt agatgccgct cgccgtcgcg ttccgaccca tcaacgtgcc
     2461 gaccggcacg tcgctcgcga agcccgaaag gccgccgatg atcgcgagat gcccgccgaa
     2521 cgcgagcgcc tgcagcgcgc gcggcagcgt gtcctggccg ccgacctcga gcacttggtt
     2581 tacgccggtg ccgcccgtga gcttgcgcac ttcgacctgc cagtccgggt tcgagcgata
     2641 gttcaccgtg ccgaacgcgc cgagctcttt cgcg
//
